LOCUS       DAOSDD010000001        55413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_360_length_55413_cov_5.198183, whole genome shotgun sequence.
ACCESSION   DAOSDD010000001 DAOSDD010000000
VERSION     DAOSDD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 55413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55413
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_55413_cov_5.198183"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK733_00005"
     CDS             <1..301
                     /locus_tag="PK733_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOJ08962.1"
                     /translation="FHIHQFGDCQVGNPSEPFKASGEHWNPTNQPHGNHAGDFPVLFS
                     NNGYARMCFFTNKFKASDVVGKSVIIHENPDDYRTQPAGASGKRIGCGVIRRPFE"
     gene            646..1908
                     /locus_tag="PK733_00010"
     CDS             646..1908
                     /locus_tag="PK733_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOJ08963.1"
                     /translation="MAVSKIIIRGIPEKAPRISDTGDVVLIFKIPVDKLSISNSSTNT
                     SSTDNSSTDNSSTDNSYYRVFVEKALWKKVSREINESTYYIIEGLPKASVSSKGLPFL
                     SVLCIDVKVINGFLEDESSPGSFKFPGELPENTEAVIPISSITIPGNINEPNTAKTRA
                     FNYFKRHGTFQNAIVVKKDTMTLVSGYANYLLAKDLNIEIVPVSYNLMTGTPSKDEYA
                     IKRILWYIPEEVTELNVKDILLTEDVHLNVQNFVFSINLKEIFKSKLIDTPISVRPTS
                     DGKYSLVTGAARYFAAKILDIEKIPAVITDMTHDEFLENRIMKHRNDNDSIDKENKAA
                     DNKKAGAAAKNEGETLLSLITIPEAFARTRPRLEKIKESMDYYKKHGKFDKPVVIKGD
                     SNLLIDGYKRYVAAKELGLKSIWTIKVR"
     gene            complement(1971..6392)
                     /locus_tag="PK733_00015"
     CDS             complement(1971..6392)
                     /locus_tag="PK733_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ08964.1"
                     /translation="MAEARWTNEQLEAITEKDCNLLVAAAAGAGKTAVLVERIIRKIT
                     CEEHPVDIDRLLVVTFTNAAATEMRERIADAISKALDKNPRSILLQRQLALLNKANIT
                     TIHSFCMEVIRNNFHYIGLNPGFRISDETEAILMKIETLDDMFDEIYDQDKLKEEFFD
                     LLESYGGNRDDQALKNMILTIYEFIQSYPWPEDWLNEKTGYFNLTDEEDFAKTLWGRV
                     LLKNTAIELMSLKEIMEKAITIIEASEGLNPYLLNFQEEKNEIESLYKTCKLSMDLIE
                     NIDTGNIADHAADDADDTGNIADHAVNDADDTGNIADHANDDADYTGNIVSADTGANA
                     STNASTAADTYTIYGNIWDVLYNCLLSFSFKRLPPCKKDVDKEQQERVKSIRDDVKKR
                     IKKLQEDIFTTCSKDIIADLQHVYPLLKYLSELVIKFGCMYAEKKREKSLLDFNDLEH
                     FCLDILTEKKSDGSFLPSDVAVEYKHKFEEILIDEYQDSNLVQEIILNVISRNDLDTP
                     NIFMVGDVKQSIYRFRQAKPELFLEKYNTYSLEKGNKYRKIQLYKNFRSRREVIDSIN
                     FIFTRIMSEDAGELNYDENEALNPGAFYEEPGETQKAGGTAELHLIELESEEGENTKS
                     PHESDGIYYSDTHENDMPGANASDNYTTDNYASYTDAYNTETPDNYQSDTDMGDSAHF
                     LYGEEPLDNIQCEARVVAERIKRLFNSEDRESGRELDRELDREFSVYDKNSRSYRPIE
                     YRDIVILLRSTKTWSDVFVEELTRQGIPVYADTGTGFFKTVEVQIILSLLQVIDNPLQ
                     DIPLLAVLRSPFVAFTPDELADIRLTDREAILYEALRKFAGVSNWEEGSPISINGNAE
                     TENAETGNIETGNIETGDVETGNIKTGDVETVDVETDSEEKTVSKLKEKTVVFLETLE
                     KWRDKALYMSTDELIWYLYTETGFFSYVGAMPGGEQRQANLRMLFEQAAKFEETSYKG
                     LFNFVNFINRLRSGRGDMGSAKILGENENVVRIMSIHKSKGLEFPVVILSGCGKGFNL
                     QDMNKSIILHQELGFGPDYVDIEKRIAYPTLAKEALRYQIKVESLSEEMRILYVGFTR
                     AREKLILTGTVKNLSKSISRWREFGSADEDTIPGHVILRGRNYLDWICSAIQKQGDGS
                     PITAKQGDSSLAPETNYRDGGHPDLKLWDISVWRKEDIIDEDKKEKQSRQASQEILEE
                     ITKMSDEILTVEKGRSNYYDEIQRRLGWEYKYYEASRIPIKVSVTELKRRFSFDTSDE
                     YKQFSIFTPTLIKKPAFLEESKGLSAAEIGTVLHFVMQHLNLNKVGDVIQIKSQVDVM
                     VEKGLLTKQQAKSINLEKVYGFFSSPIGRRMLNSTKIYREVPFNIQVSYSELFNNVKD
                     NIQGHDHDHDLDLDHETVLLQGVIDCFFEEHDELVLLDYKTDYILPGAGRDAIMQVRE
                     RYRTQIQYYTMVLEKLLHKRVKEKYVYLFWNGEIVEY"
     gene            complement(6612..10343)
                     /gene="addB"
                     /locus_tag="PK733_00020"
     CDS             complement(6612..10343)
                     /gene="addB"
                     /locus_tag="PK733_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HOJ08965.1"
                     /translation="MSLRFIYGRAGIGKSYYCLNEIKSRIESGAANSLILLVPEQFSL
                     QAERNLVKTMGSGGIIQTEVLSFRRMAYRVFNEVGGITFVRINSAGKCMIIHKILENM
                     KGQLKIFTKSAEQQGFVNTMCEMIAEFKRYNITPDALEKACERLDTKSVENTKDIDGT
                     KNIKNIDNIENTENIDDIEKIEANRTNSLLGQKLKELALIYKEYENIIGDKYKDADDD
                     LTMLYEKLDETTMFDGAEIWIDEFSGFTSQEYKVIAKLMTKVSRINICLCTDCLVDEF
                     AELCLSTGNALGSVTGPDGISGSGNGYGNSNGNIGINCVDVFYPAKNVVKKLTKIAEE
                     YNITVESPVILKRQNHRFKNSQELEHLERYFYAFPYKKYQENTGKTTDISIFTASNIY
                     TEIEHTARDIICLCRDEGLRYKDVTVVCGNLDTYDKFIRVIFTQYGIPYFIDKKRNIT
                     HHPLIQMIMSVFEIFIYNWSYEAVFGYLKTGLAGIPREEIDLIENYVLACGIRGNKWT
                     REDDWEYIPDMIWEESEKQAYDDLLIKINDIRRRITAPLVEFHRHASGRKSTSDICEA
                     LFDLLCNLEVPDRIEMYLDKFENQGELALAGEYGQVWNITMEVLDQTVELLGDERIGL
                     ERFLNIIGIGFGEYKIGLIPPALDQVLVGNIERSKSHDVKALYILGVNDGVFPAPGDG
                     EGILSDMDRGVLQSLGMELASDTRTKAFEDQYMIYSTLTTAEKYLKLSVPIADHEGKT
                     LRPSIIISRLRKIFPSILEENNIVYSGMDNEDMKLISGRNPTFNELLSIIRKTVDGEN
                     MNPLWKDVYLWFANHDKWRHRCSTAMSALAYTNLVEPVSRQKAVQLYGNPAYSSVSRL
                     EKYASCPFSYYIQYGLKVKERKIFQLTPPDIGTFLHAVLEEFSRYMAENGISWRNISK
                     EECSGIVHKISDELLEKMQGGPIKNGTKRYRILVKRLDRVLLRAVWLIVEHVKRGGFE
                     PVGYEINFGGRDGLPPINIELPSGEVIKLTGRIDRVDALETGDGTYIRIIDYKSGSKD
                     FSLSNVFYGLQIQLITYLDALWREDGEENENSRRNDRMNKEFKLPMIPGGILYFKIDD
                     PIIRNRKEFDEEEVEKAIMKQLKMKGLLLADVKVIKEMDRTIDGSSLIIPARINKGDI
                     LGKNSSAATIEQFNTLRKYVRKLLENMGTEIMRGDISISPYKNKNITSCQYCSYKSIC
                     QFDPVLKDNKYRIITDRNDDEVWSSMDMYCAENEKDCKI"
     gene            complement(10379..11653)
                     /locus_tag="PK733_00025"
     CDS             complement(10379..11653)
                     /locus_tag="PK733_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ08966.1"
                     /translation="MDSTKLSMADFMNVPDVDIMGRADLFSDFMGDIRAKHHMQYKRV
                     AFDGSEPIRRVLDPYTGEVREMIYLASNDYLNLTRHPKVVAAGREALDKYGAGAGSVP
                     LLGGTLDIHIELEEKIAKFKGCEAAIIYTSGFGSNAGTLLSLLQSEDIAILDMLAHAS
                     IIDGCKNTNLKVFKHNDLASLEHILKRVKDKYRTKLVAVDGVYSMDGDIAPLDKIIEI
                     AKYYGAYVMVDEAHATGVIGRNGKGTPEYCNVEGKVDIVAGTFSKGLGGVGGFIASNS
                     NLVQLLNFYSRTYMFSTAMTPQVTASLIAAIDVIENEPELREKLWENINYFRKNLLDL
                     GFNLGNSQTAIFPVIIGDDFIVKEICRRLHEANIYVNPVLYPAVPKKLARIRMSLMAN
                     HTTEQLDKVLNVLEDLGREFGIIGREAAGTGA"
     gene            complement(11794..14298)
                     /locus_tag="PK733_00030"
     CDS             complement(11794..14298)
                     /locus_tag="PK733_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ08967.1"
                     /translation="MLHKVFRKAISTIHSNNYNNNNYYYAIPDISEFKKEINPLKTAI
                     KFALIYGFTDILLEFLLEYVLDLLIPDINTYRYFELIKNGLHIIITTVLIFIVIHRNV
                     KLCKNAMQKTYETYDELSSTHEELVAIEEELRQQFNELEKHRNALIISEQRYELAVEG
                     SNDGIWDWDVQNDVYYFSTKWKKALGYDPQELDNTHNSLLAIMHPDDRDRALKTLNNY
                     KISKKGIYKNTYRLYCKDGSYLWILSRGVGIWDEQGSAVRIAGSHTDITEQIQLQEHL
                     RMEKEFSESILNNAQVIIVVLDNDGIMMRLNQYTTKITGYKENEFSGKKWIDIFIPHE
                     KRKHMLTVLNDIQEGKFLYNYESQIVCKDGKLLDVLWNISLLYNYDGSISGILCAGTD
                     ITYRKQLENIMYNMAYYDALTGLPNRLMLISSFNSIINEKSSTGGIMAVLFIEIDNLK
                     HINDLLGHTSGDKLMKHVSSILQKYIKSPDIVSRLGGDEFVAVLTSVKNKTEIISEVE
                     KIRLCLKEPWVIDKHEFYISASMGIAIYPEHGRDLETLLKNANTAMYNVKETVRGNYC
                     IYTQEMKEKESQYTNIANQLGKAIQNNEFTIHYQPQIDLNTGKIAGVEALLRWMNPEQ
                     GYIPPAYFIPIAEQTGYILDISYWVVKESFEQKKRWSKKGFPPVLMSINLSRKCIFHT
                     DLLRNMIELADITGIQHEQIQFEITETTVMTDINAAINVLKHIKNLGFRIALDDFGTG
                     YSSLYYIKQLPIDTVKLDQTLIQNISNINDDEVILKSLIKLAHELNLEVVAEGIETIE
                     QLEVLKKYKCDKGQGFLFYAPLLEEEVEKIFENLHI"
     gene            complement(14725..16437)
                     /locus_tag="PK733_00035"
     CDS             complement(14725..16437)
                     /locus_tag="PK733_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ08968.1"
                     /translation="MKKFKALKNLLPIVVILAMVAGSMAGCVVIEKATSSKEITTTTA
                     EEATKEQTQKNQEATAKKDDIPAYINATGFPITKEKVALKLMGSKSPLHGEWKDMEVF
                     KRMEKLTNISFDFDTPLATGYPEKKSLTFASGDYPDVFYQGGITIQDEETYGPQGVFL
                     QLDKYIDKYGPYIKKILTENPDIRKAITATDGHIYSLPYIVRTKTVAANILYINMEWL
                     ANVGMSKPTTVDQFYDVLKAFKEKDPNGNSKNDEIPLSYWKQNPAGAGSIGGILHPIF
                     YAAFSGQAGGAGFDLVGNKVVFNPVQPAFKDYLAYMRRLYSENLLDHDMFTQTMQQYI
                     AKYKEGTMGISAISLSSSLNPGQSAPYELISPLTSASNNKKVTAEYPGVFTGSFALTN
                     KCKYPEAMIRWADVFFRKVDENVEGICGLSNFLGILDYNWYFDDASRKTYTRESKVEG
                     LNPVEYISKHVMPGGFGWVVTDAVPNTDPLLLLKATESEKQYFPYTIPVYPNTVRFTK
                     EQAERMNFLKNDINTYVEQMIAKFIIGEEPLDKWDSYVNTVKSIGLDELTKIIQDGYD
                     KWNR"
     gene            complement(16502..17401)
                     /locus_tag="PK733_00040"
     CDS             complement(16502..17401)
                     /locus_tag="PK733_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ08969.1"
                     /translation="MSVIKESKSDKAFNIINVIIMTLVLIVEIYPLIFVLSASISDPD
                     LVNQGKVLLLPKGISFDGYLRVFKDKEIWTGYRNTIFYTFFGTIVNIIVTLPAAYSLS
                     RKDFYGRNLFMGLFTFTMFFSGGIIPTYLIIKGLGMRDTIWALLLPGAASMYNIIITR
                     TYFQSNIPNEMKEAAEIDGCSNTRLFLTIVLPLSTPIIAVIALFYGVSHWNSYFLALM
                     YISDREKFPLQLILREILILNEMSTEMMKTGEEIEAIAKQAKIADMIKYAVIIVSTAP
                     VLIAYPFIQKYFVQGIMVGAIKG"
     gene            complement(17510..18490)
                     /locus_tag="PK733_00045"
     CDS             complement(17510..18490)
                     /locus_tag="PK733_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ08970.1"
                     /translation="MQNTNTVASLDTKLDANAIIKPIKKGNNVWKKVIKNYELYIFIL
                     PALLYFLIFHYGPMYGIQIAFKDFIATKGIWGSPWTGMKHFNYFFKSYYFNLLIKNTV
                     GIALYSMAAGFPIPIILALLLNELKNEKFKKLVQNVTYAPHFISTVVMVGMILAFLAP
                     SNGIINGIIKALGGEPIPFMSKPSMFRSIYVWTGVWQSAGWSSIIYLAALSGIDTQLH
                     EAAIVDGATRLQRIRHINIPGIMPTMVIILILNSGSIMNVGFEKVFLMQNSLNMETSD
                     VISTYVYRMGLLNAQYSFSSAVGFFNSVINFTLLIIVNNIAKKLNETSLW"
     gene            complement(18752..21253)
                     /locus_tag="PK733_00050"
     CDS             complement(18752..21253)
                     /locus_tag="PK733_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ08971.1"
                     /translation="MLGKIVKTDTLYKYKLLISYFLIVATPLIIMTGLLYNISVVNIR
                     KEIEASNKYKLAQLKNNLDLRIKELEDLALRINVDNKLTPNNMSSNEYKRLEGLYQLG
                     MYKAQNAFIDDYYMYLKCTGNVYSASGQSTLSTLARNVYKYDKKAEQNFINEIEALKF
                     SKFIGEEKLSLANSQNVELITYIYPVSYGNYDPYGAIIFLIKKSTIGALFKDFIGNIK
                     GSMIILDKNNSVLLSENKSFKLASEDIDFIRTTEKNGIMDAKFGNKNVSLIIQTSENT
                     GLKFVAAIPGAQFFQQLINVKTLILEAFLIILAFGAILAVAFSTKLYKPIGKLMNFIT
                     LQWPEPNESYNVSLDETSDLLYDSNNYSSNDSNNYSNNNYNNCLDIIRGFKWNRSMWN
                     KSNRNKSNKNKNMSNRNKRRDEIERIGHTVETAIMQNKSLQAQINMQRPFIREQILTK
                     LLKGEVLNEGTMKSLIVFSNIKLTGFSFAVAIVDKRIKTEERHTRVFKDKVFEVIEEK
                     FNADGYVYPVELFQDDSIALILNLNISQDQDEDYLQFTYKIKQLLEDATEENVVIGGG
                     KLYNNMNKINRSFIEAYAALEENKVRSRQGILMFNDIVKSQQQQFWYPLEDQLRFVQS
                     LKQGDKAVAIEALEIMLNNVAKNQSSVLMIKYIYYDIVNMIIKTINEMKITNVDYDIE
                     ELMKFTTIEELEKETKIIVTKLCDYIKERKNTKSEELNNQILEYINKNFDDVNLSLES
                     VADEFGFSIYYLSRFFKEQTNYTFTDYVINLKMEKARELLSTTDMPIKSIVQEIGYTD
                     LTYFMKKFKKTEGITPGQYRELYSARKTNNCKENQ"
     gene            complement(21420..23456)
                     /gene="abc-f"
                     /locus_tag="PK733_00055"
     CDS             complement(21420..23456)
                     /gene="abc-f"
                     /locus_tag="PK733_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HOJ08972.1"
                     /translation="MVALDIAVREVHKYYGSEHILKGMTFEVYKGEKVGLLGKNGAGK
                     TTLFKILSGVEAYDDGTVNLSGKAGILDQIPEYPHDYTVLDVLKTSFERLSNIKNEMK
                     ELEKLMAVSSNATSNAASNTSLAVSSALSSQPSIVKRYGVLQTEFEAMGGYTIDSDIA
                     KICNGLGIDKDMQDRLFIHLSGGEKTRINLGRIILQNPVILLLDEPTNHLDINMTEWL
                     EDYLSQYKGTVLAISHDRYFLDRVVQRIVEIVDGKAEFYEGNYSYYVKEKESRYLQKL
                     QQYEQEQRKIRQLEEAARKMHQWASVADNPSMHRRAFAIEKRIEKMERTDRPTREKGI
                     KSVFKGIRFAGNDVIVIKGLRKSFNARCIIDDINLTVEKGDRIGLIGNNGCGKTTLLR
                     LITGEETPDSGFSRIGTSIKYAFLPQIVVFDNPELTVLDTVRNALIVSEEAARNRLAA
                     YHFKGEEVFKQVSTLSGGEKSRLKLCILMNSDINLLILDEPTNHLDIVSREWIEEAID
                     GYDGTLLFVSHDRYLVNRFATRIWDMEDGSISDFRGTYESYRKWKTVNNNKTTETVII
                     KAKKNINAEKNNNAKKNNNAEKNNNTEKNKKNKDNKAIENKGWKSKRERQVAGRLREL
                     ERDILILEERLKDIEIEMEQRASDYESLQTLLEEKDELNLNLDSLYMEWGELSE"
     gene            24188..25387
                     /locus_tag="PK733_00060"
     CDS             24188..25387
                     /locus_tag="PK733_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ08973.1"
                     /translation="MWEQNININEVREIRCKTTAYLGAGAMEKMNDIAKELKKMGADK
                     IIAVTGKSSYKKTGAWDYVEKACLENAIDWIIFNKISPNPSSIDVDEATKMAIDFGAK
                     AVIGIGGGSPIDAAKSVAIMVNYPQYTTADLYEYKFTPEKALPLIAVNTTHGTGTEVD
                     RFAVVSILDKQYKPAIAYDISYPLYAIDDPKLMLGLPKSQTIYTSVDAVNHIVEACTS
                     VVASPYTVLLAKETVRLIEKYLPKALENGQDLEARYYLTYASMIAGICFDNGMLHLTH
                     ALEHPLSAVKPDLAHGLGLAILLPAVVKNIYPACGEILCDVLAPIVPGLSGQASEAEK
                     ASKGVKKWLANVGITETLSTLGFTKADVDKLTHLAFTTPSLGLLLSLSPVKATEELVA
                     GIYSESL"
     gene            25735..26982
                     /locus_tag="PK733_00065"
     CDS             25735..26982
                     /locus_tag="PK733_00065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ08974.1"
                     /translation="MTGFYNEKRWVKLSLALIIALFTIINVYSVLRYNDYFLLGSIEK
                     ADNDDVKYIGSAWTLLEKGMLTYHDVNVPTVYIMPGHTIIIAAITGIFGKYGGITAFR
                     LFQVLLQALSILLVFLIGRTVFNSSVGIIAAFINATYVSEIFVTGTILTEVEFKFLLL
                     LLVYISINAIKTKKTRYYIEGGIVWGIACLFRPTIALYPVAILAAWLIYKYSFKEIFK
                     FSIIVLVIFAAIMSPWWIRNYIVFNRFIPLTLSSGNPFLQGTYINYDQSKDYTPYTPS
                     KDVIETNEIEMKTGIYRLKTYFVKYPLKYIYWYTFGKSWYLWNYPFYWKEIFGISVTM
                     ATVHHHLILIAGIPGIISIFGNKSLEGIFLITVILYFSLSYLPFYTFSRYSYPMMPLV
                     MLFSSYAVFKLYKGISAMCRTVP"
     gene            complement(27050..27595)
                     /locus_tag="PK733_00070"
     CDS             complement(27050..27595)
                     /locus_tag="PK733_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ08975.1"
                     /translation="MPKIYIMLAHTDSLVMKTIRFYTHAKYNHVSICVDERMEYFYSF
                     GRRYIRFPLIGGFIREELNKGVYLYFKNASCCIYSLDVSEEKYNVIVELIKCYGNNRY
                     KYHYNFAALLGIMINKPLKMKNRYTCSEFAATLLSTASIYTFNKDASLVVPDNFKSIP
                     GLSKLYEGPMVNFQTGTVLQF"
     gene            complement(27679..28950)
                     /locus_tag="PK733_00075"
     CDS             complement(27679..28950)
                     /locus_tag="PK733_00075"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOJ08976.1"
                     /translation="MPLIYILSAVARELLSAFIINYRFIPIYVIVVLLIKMQHEKQMG
                     IVTNWLGVPSRGIWNTIKEMMFYGSIIGFFGGVIVVSTGITLAPEVFEYLLIIMAVLI
                     LFNIRFICISYSAALLALIAVAFKISNIDVASLLALVAVMHFFESILVFAGAGRDCEP
                     VYIKHGQSIAGAFITRRIWPVPVIFLTFLAQRFGDVTLNSINTIKVDWWTLFNSSAVH
                     AGALAIGLDCAVSALCYTDIAIARKPENKSRETAIWLLIYSILLFILAVISRNISLFK
                     VMGALFALGGHEFIFLYGRYREMNNSPLFIPVRRGVRVLDVLPDSHAWRIGIRRGDII
                     LSINSRDVQTEEGITEALRTFPTFIWVKLLDVDGIERTYEYKCYPDGINHLEILTVPR
                     ENEVTYNMSYFENYNVLKNLVKRFRGNSKSI"
     gene            29263..31050
                     /locus_tag="PK733_00080"
     CDS             29263..31050
                     /locus_tag="PK733_00080"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOJ08977.1"
                     /translation="MSDFIINFIKESPYILADGAMGTYFAETSGISAKRCEFANIEAP
                     EIIKRIHEEYIAAGSQILRTNTFAANTISLKTDFETIKEILIKGFEIASSAAKNKGVH
                     AIADIGPIPEAEGHDTLQEYKNIVDIFFECGAKSFIFETFSSYEKLIEVCKYIKRRDS
                     TTFILTQFAVSPDGYTRKGISGKRILAAVCNSDVIDSYGFNCRSGPMHLLEYVRNLNI
                     PAKHLEMLSIMPNAGYPTTENERTLFLNNPEYFAHIMADIKNLGVKILGGCCGTTPRH
                     ILETAKALGNRRETSIRVDAIAKQESTRVKVKNSFREKLDLGELTIAVELDPPINANI
                     TNMIEGAKALGECGADIITIADCPLSRARVDSSILASRIKRETNMETLPHLTCRDRNL
                     NAIKALLLGLHIEDIRNVLAVTGDPIPEAERGDVKGVFNLNSYMLTEFIRELNHTEFA
                     NDEIFIGGALNVNSVNFDVELKRAETKVNKGTEFFLTQPVFGQEAVSALIKAKNTLNA
                     KILGGILPIVSHRNAIFLNNEIPGIKVPKDIIRQFEGKERDEAESTGINIAIETCKKI
                     LPYVDGFYIITPFNRWRMVCAIIKKIRNA"
     gene            complement(31232..32047)
                     /locus_tag="PK733_00085"
     CDS             complement(31232..32047)
                     /locus_tag="PK733_00085"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOJ08978.1"
                     /translation="MHDIQDIKDIKNINSINVNRPEIGIILGSGLGLLAEDIENQTTI
                     PYKSIPHFPVSTVEGHKGQLILGALSGKHVVCMQGRFHFYEGYSIHEVVYPIRVLQKL
                     GITKLIVTNSAGGINTSFSPGDLMLIEDHINLMGVNPLISMNSESFGPRFPDMTKAYD
                     PEHMEKAEKAAKRLSIRLRKGVYAAMTGPSYETPAEIRYLRAIGADAVGMSTVPEVIA
                     ANHGGISVLGISCITNMAAGVLNKPLSHRDVVQVANRATSDFVKLIKEIIIKL"
     gene            complement(32364..33551)
                     /locus_tag="PK733_00090"
     CDS             complement(32364..33551)
                     /locus_tag="PK733_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOJ08979.1"
                     /translation="MARTVGTVARGIRAPIIKSGDDLVNIVVDSILKSAQEENYHLND
                     RDVIGVTESLVARAQGNYASINDIAFDINRKFNGDIAVVFPILSRNRFSIILKGIAMT
                     GKKIYLFLNYPSDEVGNRLIDIDKMDELGINPYTDTLSEKKFRELFGEKVVHPFTGID
                     YIKAYKEIAQNGNIDIYLTNDPKAALNYSSEILVANIHERSRTKRILLNSGAKKVYGL
                     DEILNESVNDSGFNPDFGLLGSNMSTEDKVKLFPRDCNRTVIEIQNKLIEKTGKHIEV
                     MVYGDGAFKDPVGKIWELADPVVSPGYTDGLCGTTNEIKLKYVADNEFNSLNKSEIDE
                     AVKRKIKEKGNNMVGKAESLGTTPRNLTDLLGSLCDLMSGSGDKGTPIVLIQGYFDNY
                     ATE"
     gene            complement(33739..34650)
                     /locus_tag="PK733_00095"
     CDS             complement(33739..34650)
                     /locus_tag="PK733_00095"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOJ08980.1"
                     /translation="MENGFYINGYASKDIYVYSWNDCCKPRAVIQVFHGMAEHAGRYG
                     RFASYMNSKGFAVYANDHRGHGKTAGKLEEVGYIGEDGFNRIVEDEYIITKKIKEIHP
                     DIPIIILGHSFGSFVAQEYIIKYGESISGTILSGSSMTKGSEVAVGKIIAAISRNVFG
                     DRKKAYLLNKLSFGSYNKMIKNPVSPFSWLSRDEAEVKKYDEDPYCGKVFTAGFYYYF
                     FEGLAKLYCDERLGSIPYELPLLIISGDQDPVGKYGSGVKKLYENYKSIGMRNIEIKL
                     YQGARHEILNEINRDEVMEDISAWINR"
     gene            complement(34717..36213)
                     /gene="glpK"
                     /locus_tag="PK733_00100"
     CDS             complement(34717..36213)
                     /gene="glpK"
                     /locus_tag="PK733_00100"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOJ08981.1"
                     /translation="MKKKYILTIDQGTTSSRAVLFDMETVTIKNIKNKPFKQIYPHPG
                     WVEHDPVEIWTGQLEVLKSVVEESGISPDEIAGIGITNQRETVVVWDKITGKPVYNAI
                     VWQCRRTADLCNRLKFDGLQDVIKEKTGLVIDAYFSGTKIQWILENVPGAMERAGKGE
                     LLAGTMDSWLIWNLTGGKLHVTDYSNASRTMIYNIRKLQWDKELLKQMGIPECMLPTV
                     VPSSGIIGDTSQEILGIPIPISGIAGDQQAALFGQACFDVGTAKNTYGTGCFILMNIG
                     KQPVKSEHNLLNTIAWVIGNEVEYALEGSVFNAGSAIQWLRDEIGLIKTAHESDIEAE
                     KVADTGGVYMVPAFTGLGAPYWDMYARGTIVGITRGTKRQHLIRAVLESIVYQSMDVF
                     EAMKIDSGIELKELKVDGGASVSDFLMQFQANILSINVKRPQITETTALGAAYLAGLG
                     AGLWHSKEEIADKWALDKVFTPAMSDEIRMNKYLRWKNAVERSVKWDV"
     gene            36490..37818
                     /locus_tag="PK733_00105"
     CDS             36490..37818
                     /locus_tag="PK733_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOJ08982.1"
                     /translation="MKKRNINILLISLSILILITAGALNLGQYIKPKNPDNKNLDSKN
                     PDFKKPDSKSPDIKNPDFKSPDSKNPYSKLEYQGESSPVTYRFPGEFENQQAIWMQWP
                     SQIYNESSHPVNPVMINIIKAFDPYIRVNVASQDTDEIVSIENMLKQSGYTGSNVHFY
                     VVKHLSIWARDVGPIFVKDSNNKFHVVDFVFNNYSRGGSQYYIDIENQVHKQIANLYG
                     YPLISTNLISEGGAIESNGKGTLMLTESVALKRNSKLTKQEIENEYKRVLGVSKIIWL
                     KKGLAEDDSITTGHINEIARFANPDTILLAQVLPADRYTNSDSNESYLRLEENYDILQ
                     NATDQDGKSFKIIRVPMPPTIYQKPVETGKIPVRSYLNYAVTNNAVLMQTYWRPGRSD
                     TLKAAEDDVKAILEGVFPGRDIVGIDSEALNLWGGGIHCVTQHMPECISQ"
     gene            complement(37866..38633)
                     /locus_tag="PK733_00110"
     CDS             complement(37866..38633)
                     /locus_tag="PK733_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ08983.1"
                     /translation="MILCAKNLSLGYGRKLVINNINMEIEKGKISAIIGPNGSGKSTI
                     LKAFSRIIKPCDGKVLLNGKDIHKMDTKEVAKQISILSQVREAPDDFTVEDVVKFGRF
                     PHLSFGQKFTVKDKEIVSWAIEKTGLQDFRGSLLKKLSGGEKQRVYIAMALAQKTNIL
                     LLDEPTTFLDVSYQTGILDLIKDLNESLNLTVVMVLHDINQAAKYSNKIYALKDGKIY
                     DSGKPEKVLTKDLFRYVFDVEAHIIYDELNNRTVYLF"
     gene            complement(38630..39664)
                     /locus_tag="PK733_00115"
     CDS             complement(38630..39664)
                     /locus_tag="PK733_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ08984.1"
                     /translation="MKSNFFNILKDNRNIKNIVILVLMVFLCLAGLIISLENGAVKLS
                     PQEIFRAIFVEKDTTNHQVIWNVRLPRALTGALVGICLSLSGVILQGVMGNPLASPGI
                     LGISSGAGLAAFIIFILFPNLYFIAPIAAFLGALGTAAVVYLLAWDGGLQPLRLILSG
                     VAVSSILSGGINALMIFYPERAIGVYGFMVGGLSAKTWHHFKMILPYAFLGSIFSLLL
                     AKKLNILLLGDETAISLGLRVEKVKFIFIILSALLAASAVSVAGLLSFVGLIVPHIAR
                     LLTGGADHKYLLPASILLGAFTVMICDTIGRVIISPVEIPVGIIMSTLGAPFFLYLLA
                     MGRSRRGGKK"
     gene            complement(39850..41034)
                     /locus_tag="PK733_00120"
     CDS             complement(39850..41034)
                     /locus_tag="PK733_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ08985.1"
                     /translation="MEKTNMQRKISSSAFVLVLTIFLNGCGHKTIDKAKTTYVQSGTS
                     DILNDGHISKKNDTDINDGKDIFDSVDILDDIDISDDTVSFIDAIDDRVTIRKRPERV
                     VCLYDSYLELWDLAGGKVIGRVESKRTVPEPSKDAEVIGKTGAASIEKIIALEPDLVI
                     LSPTMNGQNNLIDMLKQNKISYIAIKYQNVMEYLKVLKIFTELTGRNDLYIKNGVEVV
                     NRVKEIVFKVAESNRIINGDGAEVNGTVNNELENNSNERKTIKILLLFATSRGITVKL
                     PNSSVGSMLEDLGTMNIASGLAGGFGVLDAETQIFSLEKIIEENPDFIFVESMGGTVQ
                     ETKNMIDTELGKNNAWNSLDAVRNCRLIYLEKELYTYKPNTRYPEAYEKLARTLYPEV
                     FR"
     gene            complement(41198..42046)
                     /locus_tag="PK733_00125"
     CDS             complement(41198..42046)
                     /locus_tag="PK733_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ08986.1"
                     /translation="MDKQQIAKLKSEGFLAQQQQGYFSVRVVTNVGNMDSRNMEKLSE
                     IAEKYGRGYMGFTTRLCVEIPWIKEEDIDNVKQELKDAGMRHGGTGKRVRPIVPCKGT
                     VCTHGIIDTQGICMKLHEKYFAMELPAKLKIGAVGCPNNCAKASLNDIGFMGQAYVKI
                     DREKCKNCKLCVGACKTKAIEYVENKTEINRDKCLNCLVCSKTCPFGAITVETAGVAL
                     FIGGRFGRQWQIGRRVDRIFKVDEIEEVTGKIIDYFKENAKDGERLALMIERIGWDKV
                     QKDLFG"
     gene            42319..43311
                     /gene="mgrA"
                     /locus_tag="PK733_00130"
     CDS             42319..43311
                     /gene="mgrA"
                     /locus_tag="PK733_00130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HOJ08987.1"
                     /translation="MAYVPDANRYTEMKYNRCGRSGLMLPAISLGLWHNFGGVDSYEN
                     SRALIHKAFDLGITHFDLANNYGPPPGSAEENFGRILKSDFTGYRDEMIISTKAGYRM
                     WPGPYGDWGSRKYILSSLDQSLKRMGLDYVDIFYSHRPDPETLLEETMSALDQAVRQG
                     KALYVGISSYSAEQTKNAADILKNLGTPCLIHQPSYSMLNRWIEDGLQDVLDSEGIGS
                     IAFCPLAQGLLTTRYLSGIPQDSRAAKSWGFLKKERITEDILTKIKHLNDIAALRGQT
                     LAQLALAWVLRGGRVTSALIGASKVSQIEENIMALNNLNFSKEELDRIDQILQG"
     gene            complement(43537..43830)
                     /locus_tag="PK733_00135"
     CDS             complement(43537..43830)
                     /locus_tag="PK733_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ08988.1"
                     /translation="MKRKTVIVIAVTVFVIFALTGCIPGDGKYTSQKPAGFFSGIWHG
                     WVAPISLIIGLLDRNIRVYEPVNTGWLYDFGFYIAIISGFGGLSLFRNKKKKD"
     gene            complement(43972..44256)
                     /locus_tag="PK733_00140"
     CDS             complement(43972..44256)
                     /locus_tag="PK733_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ08989.1"
                     /translation="MIFKKRSRPDINKNKCNYSNVINSKSMDETKANMSGNSGDGCSE
                     DSEDALKLMTFKDYVALFMALLSILLPWVLIFGAIFALVVFLLTRFWLKA"
     gene            complement(44275..45243)
                     /locus_tag="PK733_00145"
     CDS             complement(44275..45243)
                     /locus_tag="PK733_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ08990.1"
                     /translation="MSELLLEVENLKKYFPIKGGVLQKTVGYVRAVENVSFTLKKGET
                     FGLVGESGCGKSTAGRTILRLYEKDGGKVLFKGKDIHALNKKELTELRPKMQIVFQDP
                     YSSLNPRMTVGDIVGESLIEHGRYTKRNVKEHVLEILERCGMLPYHVDRYPHEFSGGQ
                     RQRIGIARALALEPEFIVCDEPVSALDVSIQSQIINLLSDLQSEFGFSYLFISHDLSV
                     VKHVSHRIGVMYLGSMVETAETNELFQNPVHPYTKALLSAVPIPDPTLRKNRIILTGD
                     IPSPANPPSGCKFHTRCPYAFEKCRKENPQLKDVGGGHMAACHLTK"
     gene            complement(45246..46217)
                     /locus_tag="PK733_00150"
     CDS             complement(45246..46217)
                     /locus_tag="PK733_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ08991.1"
                     /translation="MSNNLVEFKNLHTYFFTDAGIVKAVNGVSFNIKKGETVCVVGES
                     GCGKSVTALSLTRLIQSPGKIVEGEILYNGTDLLKLTTAQMHQINGSKISMIFQEPMT
                     SLNPVFTVGDQISEKIMLHEKLSKSEARQKSIEMIKVVGIPRAEGIYKSYPHELSGGM
                     RQRIMIAMALACNPELLIADEPTTALDVTIQAQILDLLLKVKKEFGMSIMLITHDLGV
                     VAEVADYVIVMYAGKVVEEGPVLEIFKNPKHPYTIGLLKSKPQIDQTQGRLYSIPGNV
                     PNPIGLPEMCYFAERCENCMEICKTTFPPLIKSENGHKVACRLYKEV"
     gene            complement(46240..47133)
                     /locus_tag="PK733_00155"
     CDS             complement(46240..47133)
                     /locus_tag="PK733_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ08992.1"
                     /translation="MERNRQDLIQTPWSIALRQFRRNKLALVGMGILAIFIILCFYGP
                     VFSNFALLETKVTIAKQKPSFKYIFGTDAIGRDIFTRLMYGGRISLTVGFVAVLIEIV
                     IGTAIGGIAGYYGGKIDSALMRIVDVFLSLPYLPMVIILGTVMSDLDVPPQKRIFFVM
                     LILGFLGWPSLARLVRGQILSLREQEFMLAAEALGLSDRRKIARHLIPNVIPQIIVSA
                     TLGIGGAILSESALSFLGLGVAMPYPSWGNMIQTVKDSYDFAYRWWLWIPSGVCIFVT
                     VLAINFVGDGLRDAFDPKMKR"
     gene            complement(47665..48627)
                     /locus_tag="PK733_00160"
     CDS             complement(47665..48627)
                     /locus_tag="PK733_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ08993.1"
                     /translation="MWQYLIRRILQMIPIIIGISLVIFLIINLVPGNFIEAKANPKMS
                     AEQIEHLKDLYGINDPIPVKYWKWLKGAAVGDFGDSFIYIKPVSEVINTFVWNSFILA
                     LFAFILEILIAIPIGIISATKQYSKTDMIFTVFALMGISIPSFFMGLLLKKIFAVDLK
                     ILPLANMTTPGANYTGIRYLWDVISHLIMPCLVLAFSGVGSLMRFTRTAVLEVIKQDY
                     IRTARSKGLSEKTVIYKHALRNALIPIVTIIGLSLPGLFSGAIITESIFGIPGIGKIA
                     LDAVNKRDYPLLMGFSMFVAVLTLIGNLLSDILYAVVDPRVKLK"
     gene            complement(48775..50553)
                     /locus_tag="PK733_00165"
     CDS             complement(48775..50553)
                     /locus_tag="PK733_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ08994.1"
                     /translation="MKKYLPILAIALVVIIAITAVWLGKGKNNDETSDKEVSSETQVG
                     SEKKEEGPKEESIDLTKLGVDFIKASNAAKSPAVALDRKDTMVIGVTEFNGIFNPFYA
                     ETAYDIYGNILMFDSLIEADFDGSPIPGAADYKVSDDGLKYTFTLKEDLKFWDGTPVK
                     AEDVEFAYYVLSDPSYDGPIDISSAFIKGFETYKDGIAEKIEGVKVINDTTIEVTCDK
                     PSGPAIWSLNMPIVSKKVYGVDFKKGQLEKVKANIGNPMGTGQYKYVEYKEGASLELV
                     ANENYFKAAPKIKNVVFSVTPSGEELQRVMLGETDIDMGDVSAENIQEAKNTEFINVY
                     RFPTNGYGYVGINNTVKKYQDKKVKQALYYAMNRKDVVAQIYGDYGSVINIPQSKVSW
                     AYNDEGINAYDFDLDKAGKLLDEAGWKLNANGKREKDGEVFTVKFTAMPGHPVTDILL
                     PVMKDDYAKLGIDIVIETLDWPTLVDKVNKKQVEMWFMAWGLTPDPDAKNIYHSQGAQ
                     NRYQYKNPQADKLIDDGINETDVAKRKAIYKDLYKVLNDDLPCSWVYQRSDMWVANAR
                     VKGPELSSYREYLYNLYKCELVSPAQ"
     gene            51072..52451
                     /locus_tag="PK733_00170"
     CDS             51072..52451
                     /locus_tag="PK733_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ08995.1"
                     /translation="MTSFKKLIISGTLSLGILFCSAGITHAQEISYKVQQGDTFWIVS
                     QKYGVPVNRLMQVNKATQNTILYIGQNILIPTDYEIIHTVVAGDTYWIISQKYGVDLY
                     SLMAANKATEKTILYIGDKVVIPPKPQAPQYTLYTIQAGDTYWIISRKYKLDVKYLMA
                     YNGANENTILYIGQTIKIPQPGVSGSTASGAGTASGAGTTSGASTASGASTASGASTA
                     SGTISGTTPVQVPVQTLTGTEPYITYTQYTVQKGDILWDIAIKFGIPQTELLKANNLT
                     ELSIAYIGDILKIPVHHVPVKATPGDKYGEYLDWWTEAQYVVPVGSEFEVIDFYTGRS
                     FKAKRTTGANHADCEALTISDVSKMKEIWGGNLSWVSRPVIIKYNGRKIAASAASFPH
                     AGNDNAPGGIYTTWRSGDYGAGYNLDWVKDNGMDGVFDIHFLNSTRHLDGKIDLSHQG
                     NIKPAAGIK"
     gene            complement(52795..53385)
                     /locus_tag="PK733_00175"
     CDS             complement(52795..53385)
                     /locus_tag="PK733_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ08996.1"
                     /translation="MSKKHKLIRNTGILLTLLFIHFMFSSASLTALSAHKKSEKTANY
                     GPSKVIQTQNFKYGKLFLCKYDKWFSCNTVKKGCLGMWYPGNQVHGKENDVSRSIDYY
                     WHGSTYRDDYMLWMLYGVVNDNRIISVKMEAELHGEKKILEYPQLYDNMFLFTWNSKE
                     TDYKIKRLIGFDKNLNAIYEVEMGAYFKSEEIFTRS"
     gene            complement(53378..53590)
                     /locus_tag="PK733_00180"
     CDS             complement(53378..53590)
                     /locus_tag="PK733_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ08997.1"
                     /translation="MLHFNVYDDLGNEYYGGSSSIGNIFGTRHIIKIEKINETAAVLI
                     LDYDYYNRKMRFEIPLYGGYGGDSFE"
     gene            complement(53718..54506)
                     /locus_tag="PK733_00185"
     CDS             complement(53718..54506)
                     /locus_tag="PK733_00185"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HOJ08998.1"
                     /translation="MIDKKNILGKFNKIEDKMLVTRIIDKAAQAEKSWIAACSDFLDP
                     YQRKTIESALHGVKDVEYKFDGGYKEAERTIVIFSPEQMSNHPSLSDYLTLLEIKLKT
                     ERYLSHRDYLGALMGLGIKREKIGDIIVDERSCSIIVLKDVADYILYNLNKIGHINVY
                     TRLGKLENLEISVPRTKEIKCTVPSLRLDCIVSNGFTISRSKVAPLFSSGRVYLNWEP
                     EDSLSAQVKEGDTVSVKGKGRVVIDEIGGTTRKGRIAVILKKLI"
     gene            complement(54852..>55413)
                     /locus_tag="PK733_00190"
     CDS             complement(54852..>55413)
                     /locus_tag="PK733_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOJ08999.1"
                     /translation="SNIVSGISPENKEQGILQISDIILDYISDREKQGKVVDMVEGKI
                     KASEPDIKGNLLKFVSNKLDSILNSTALYDNIYLIINRLIDMVMNKPVSNILDNIDEN
                     MFENIANFIKSFLGNFVKVKLPNIVELLNIPKVVEDQINSYDVAFAEEIIIEIANKEL
                     KAITWLGALLGGIMGILSPLLQLLYK"
BASE COUNT        16806 a        11150 c         8669 g        18788 t
ORIGIN      
        1 tttccatatt catcagtttg gagattgcca ggtaggtaac ccatcagaac ctttcaaagc
       61 ttccggagag cattggaatc ctacaaacca gccacatggg aaccatgcag gcgactttcc
      121 cgtcctattc tcaaataatg gttatgcaag gatgtgcttc tttactaata aatttaaggc
      181 atctgatgtc gtaggaaagt cagtaattat acatgaaaat ccagatgatt acaggacaca
      241 acctgcaggc gcatcaggaa aacgtattgg atgtggagta ataagaaggc ctttcgaata
      301 agagaggctt tttatattga tatgttttat tttattacag gaaatatatt ttcaaaatta
      361 aaaaatacac tttttttgtt gtattgtatt agttgtattg tattatatgt atatattata
      421 ctttacagct tttttcataa acaactgttt aaacttgcat taaagtacta ataattatac
      481 aatggtgttg taatattaag gtttaatatg aaaacatatt attgatgtgg attattataa
      541 ataataatta cgttttatat attaacaatt atgttttata tgtaaaatgt agtatttgct
      601 tatttacatt aatttattaa tattataagg agtgatgatt taacaatggc agtttctaaa
      661 ataataatta gaggaattcc cgaaaaagca cccaggataa gtgataccgg agatgtagta
      721 ttgattttta aaatacctgt ggacaagcta tcaataagca attcatcaac aaatacttca
      781 tcaacagata attcatcaac agataattca tcaacagata attcgtatta cagggtattt
      841 gttgaaaagg ccttgtggaa aaaggtttca agagagataa atgaatcaac ttattatatt
      901 attgaaggtt tacccaaagc aagtgtaagt tcaaaagggc tgcctttcct atcagttcta
      961 tgtattgatg taaaggttat taatggtttt ttggaagatg aatcttctcc aggttccttt
     1021 aaattcccgg gagagcttcc tgaaaatacc gaagctgtta ttcctatttc atctattaca
     1081 atacccggta atattaatga acccaataca gccaagacaa gagcctttaa ttattttaaa
     1141 agacatggaa cattccaaaa tgctatagtt gtgaaaaaag acactatgac tctggtttca
     1201 ggttatgcaa actaccttct ggccaaagac ttgaatattg aaattgttcc agtttcttat
     1261 aacctcatga caggaactcc gtcaaaagat gaatatgcaa ttaaaagaat tttgtggtat
     1321 atacccgaag aagttactga attaaatgta aaagatattc tcctaacaga agatgttcat
     1381 ctgaatgtgc agaactttgt tttcagcatt aatctcaagg aaattttcaa aagcaaactt
     1441 atagacactc ctatttcagt aaggccgaca agcgatggaa aatatagtct ggttacaggt
     1501 gctgccagat attttgctgc gaaaatcctt gatattgaga aaatacctgc agtaataaca
     1561 gatatgaccc atgatgaatt tttagaaaat aggattatga aacatcgtaa tgataatgat
     1621 agcatcgata aagaaaacaa agctgccgac aataaaaaag ccggagcagc agcaaaaaat
     1681 gaaggagaaa ctctcctttc attaattaca attccggaag catttgcaag aacaagacct
     1741 aggctggaaa agataaagga aagtatggat tattacaaaa aacatggaaa attcgataag
     1801 cctgttgtta ttaaagggga ttctaacctt ttaattgatg gttataagag atatgtcgca
     1861 gcaaaagagc tgggtcttaa atctatctgg actataaagg tcagataatt cgtctcaaca
     1921 atgtgtgtat tcaatattca atgtgtgcat tcagtattca atgtgtgcct tcagtattcc
     1981 actatttctc cattccagaa taagtaaaca tacttttcct taaccctctt atgaagcagt
     2041 ttttccagaa ccattgtgta atactgaatc tgtgttctat acctctccct tacctgcatg
     2101 attgcgtccc gtcctgctcc tggtaatatg taatcagttt tgtaatccag gagcaccaac
     2161 tcatcatgct cttcaaaaaa gcagtcaata acgccctgaa gaagtactgt ttcatggtca
     2221 aggtcaagat catggtcatg gtcatgccct tgaatattat ccttcacatt gttgaataat
     2281 tcgctataac tcacttgaat attgaatggc acttccctat atattttcgt ggaattcaac
     2341 attcttctgc caatgggcga agaaaaaaat ccatatactt tttcaagatt aattgacttt
     2401 gcctgctgct ttgttaataa gcccttttca accattacat cgacctggct ttttatctgt
     2461 ataacatcgc ctaccttgtt taaattaaga tgctgcatta caaaatgcag tactgtccca
     2521 atctcggctg cactaagccc cttgctttct tccaggaaag caggcttttt tataagggtc
     2581 ggagtaaata tcgaaaactg tttgtactca tctgaagtat caaaagaaaa tctcctttta
     2641 agttcggtaa cagatacttt aattggaata cgtgatgcct catagtattt atattcccag
     2701 cccaatcttc tctgtatttc atcgtaataa ttactgcgcc ctttttcaac tgtcaggatt
     2761 tcatctgaca ttttggtaat ttcctcaagg atttcctgac tagcttgacg agattgtttt
     2821 tcttttttat cttcgtcaat gatatcttcc ttgcgccaaa ctgaaatatc ccataatttt
     2881 aagtcgggat gaccaccatc tctatagttt gtttcaggag caagagagct gtcaccttgc
     2941 tttgcagtga ttggagaacc gtccccctgc ttctgaatgg cagaacaaat ccaatccagg
     3001 taattcctgc ctcgtaaaat aacatgtccc gggattgtat cttcatcagc agaaccaaat
     3061 tctctccacc gtgaaatgct tttgcttaag ttttttacag taccagttag tatcagtttt
     3121 tctcttgccc tggtaaagcc tacatataaa attcgcattt cctctgataa gctttctact
     3181 ttaatttgat accgtaacgc ttcctttgca agtgttggat atgctattct tttttcgata
     3241 tccacatagt cgggtccaaa tccaagttcc tgatgcagga ttatgctttt attcatatcc
     3301 tgaaggttaa atccttttcc gcagcctgat agtattacta cagggaattc gagacctttg
     3361 cttttgtgta tgctcatgat tcttactaca ttttcatttt ctccaaggat tttagcactt
     3421 cccatatcac ccctgccgct tctgagtctg ttaataaaat tcacaaaatt aaaaagtccc
     3481 ttatagcttg tttcctcaaa ttttgctgcc tgttcaaaga gcattctgag attggcctga
     3541 cgctgctcac ctccgggcat agctcctaca taactgaaaa agcctgtttc agtgtacaga
     3601 taccaaataa gctcatcagt agacatatat aatgccttgt cccgccattt ttcaagagtt
     3661 tcaagaaata ctacggtttt ttcctttaac ttgctgacag ttttttcttc gctatcggtt
     3721 tcaacatcga cggtttcaac atcaccggtt ttaatatttc cagtttcaac atcaccagtt
     3781 tcaatatttc cagtttcaat atttccagtt tcagcatttt cagtttcagc atttccatta
     3841 attgatatgg gactgccttc ttcccagttg ctaacaccgg caaactttct gagagcctca
     3901 taaagaattg cctccctatc tgtaagcctt atatctgcaa gttcatcagg agtaaaagct
     3961 acaaacggtg aacgaagtac agctagcaga ggtatatcct gtaaaggatt atcaataact
     4021 tgcagcaatg aaaggataat ttgtacttca acggttttaa aaaaccctgt ccctgtatct
     4081 gcataaacag gtataccctg gcgtgtaagc tcttccacaa atacatcaga ccaggtttta
     4141 gttgagcgaa gaagtattac tatatcccta tattctattg gcctataact cctgctgttt
     4201 ttatcataaa ctgaaaattc cctatctaat tctctatcta attctctacc tgattcccta
     4261 tcttcggaat taaagagtct cttgattctt tctgctacaa ctcttgcttc acactgtata
     4321 ttgtcaagtg gttcctcacc ataaagaaaa tgtgcagaat cacccatatc agtatcagat
     4381 tgataattat caggcgtttc agtattatat gcatcagtat aggatgcata attgtcagtt
     4441 gtatagttgt cagatgcatt agcaccgggc atatcatttt catgtgtatc agagtaatat
     4501 atgccgtcag attcatgggg tgattttgta ttttcccctt cctcactctc cagttcaata
     4561 agatgtaatt ctgcagttcc acctgctttt tgtgtttctc cgggttcttc gtagaaagca
     4621 ccagggttta aagcttcatt ttcatcataa ttcagttctc ctgcatcctc agacattatc
     4681 cgggtgaaaa taaagtttat actgtcaatt acttccctac ggcttctaaa attcttgtat
     4741 aactgaattt ttctatattt attacccttt tcaagtgaat atgtgttgta tttttccagg
     4801 aacagttcgg gtttagcctg ccggaagcgg tagatactct gttttacatc cccaaccata
     4861 aaaatgttgg gggtatccag atcatttctt gatatcacat taagtattat ctcctgaacc
     4921 aaattactgt cctggtactc atcaataagt atttcttcaa atttatgttt atattccaca
     4981 gcaacatcag atggtagaaa actcccatca gatttttttt cagtcagaat atccaggcag
     5041 aaatgttcca ggtcgttaaa gtccaaaagg gacttttctc ttttcttttc agcatacata
     5101 cagccaaatt taattaccag ttcagaaagg tatttcaata gtggatagac atgctgcaag
     5161 tctgctataa tatcttttga acaagtagtg aaaatatcct cctgcagctt ttttattctt
     5221 ttctttacat cgtctcttat ggactttaca cgttcctgct gctccttatc cacatctttt
     5281 ttgcagggtg gaagtctttt aaaactaaat gataggagac agttgtaaag gacgtcccag
     5341 atgttcccat aaatggtata agtgtcagcg gcagtactgg cattggtact ggcattagca
     5401 ccagtgtcag cgctgacaat attaccggta taatcagcat cgtcgttagc atgatcagca
     5461 atattaccgg tatcatcagc atcgttgaca gcatgatcag caatattacc ggtatcatca
     5521 gcatcgtcgg cagcatgatc agcaatatta ccggtatcta tgttttcaat taaatccata
     5581 ctcaatttgc aggttttata taaagattca atctcatttt tttcttcctg aaagtttaaa
     5641 agataaggat ttaggccttc tgaagcctca attatagtga tggccttttc cattatttct
     5701 ttcaggctca tcaattcaat tgctgtattt tttaaaagta ctcttcccca taatgttttg
     5761 gcaaaatcct cttcatcggt caagttaaaa tacccggtct tttcattcag ccaatcttca
     5821 ggccagggat aactttgaat aaactcatat atggttaata tcatgttttt tagggcttga
     5881 tcatccctat ttccgccata ggactcaaga aggtcaaaga attcctcctt aagtttatct
     5941 tggtcatata tttcgtcaaa catgtcatcg agtgtttcta ttttcattaa tatggcttca
     6001 gtctcatctg atattctgaa cccggggtta agtccaatat aatggaaatt atttctaatt
     6061 acctccatac agaaagagtg tattgtcgtt atgttggcct tattcaggag ggccagctgt
     6121 ctttgaagca atattgaccg gggattttta tcaagggctt tagaaatagc atccgcaata
     6181 cgctccctca tctcagtggc ggcagcgttg gtgaaagtta ctacaagaag cctgtcgata
     6241 tcaaccgggt gttcttcgca ggttattttc ctgattattc tttctaccaa aaccgccgtt
     6301 ttgcctgctc ctgctgctgc tgccactaga agattacagt ctttttctgt tattgcttca
     6361 agttgctcat tagtccatct ggcttctgcc aattctttca gtcctttcca ttctagtttc
     6421 gcatatcaat ttatacttta cattagtaca tttatgatta tatttaatgc ttcatttggc
     6481 atttcgtatt ctacgttttg tactatattt tacatttcat atcacacata acattttata
     6541 attatatatg attctactgc attaactcaa tttcactaca aaaagtccaa tgctgttaca
     6601 ttatgcttta atcaaatttt acagtctttt tcattttcgg cacagtacat gtccatcgaa
     6661 ctccaaacct catcatcatt cctatcagtt ataatcctgt acttattatc ttttagaaca
     6721 ggatcaaact ggcatattga tttatagctg caatattggc atgaagttat atttttgttc
     6781 ttatatgggc taatggaaat atcacctctc attatttccg ttcccatatt ttcaagaagc
     6841 tttctcacat atttacggag tgtattgaac tgctcaatag tagctgctga agaattcttt
     6901 cccaaaatgt ctcccttatt tatccttgca ggtattatga gagaagaacc atcaattgtc
     6961 ctgtccatct cttttattac tttcacatct gcaagtaaaa gacctttcat tttcagttgt
     7021 ttcattatag ctttttcaac ttcctcctca tcaaactcct ttctattcct tattatagga
     7081 tcgtcaattt taaagtataa aattcctcct ggtatcatag gaagtttaaa ttctttattc
     7141 atcctatcat ttcttctact attttcgttt tcttctccat cctctctcca gagagcatca
     7201 agataagtta taagctgaat ctgaagtcca taaaatacat tagaaaggct aaagtcctta
     7261 ctgccggatt tataatctat tatcctgata taagtaccat ccccggtttc aagtgcatca
     7321 actctgtcaa ttcgtcctgt gagttttatt acctctccgg atggaagttc aatatttata
     7381 ggaggcagtc catccctgcc gccaaaattt atttcataac caacaggctc aaagcctcct
     7441 ctttttacat gctccactat aagccataca gctcttaaaa gtactctatc cagccttttt
     7501 acaagaattc tgtaacgctt tgttccgttt ttaattggtc ctccttgcat tttctccagc
     7561 aattcatcgg atattttatg tacaattcca gaacactctt ctttgctgat attccgccag
     7621 gatataccgt tttcagccat gtatcttgag aattcctcca atactgcatg caggaacgtg
     7681 ccgatatctg gtggagtcag ctgaaaaatt ttgcgttctt ttaccttcaa accatattga
     7741 atataatacg aaaagggaca tgaggcatat ttttccagcc tggacacact ggaatatgca
     7801 gggtttccat agagttgaac agccttttgc ctgcttacag gctcaaccag attggtatag
     7861 gccagtgccg acattgcagt agaacatcta tgccgccatt tatcatgatt tgcaaaccat
     7921 aggtaaacat ccttccataa tgggttcata ttttccccat caacggtctt tcttataata
     7981 gaaagcagct cattaaatgt tgggttccgc cctgatatga gcttcatatc ttcattatcc
     8041 attccggaat atacaatatt gttttcctct aaaatgctag ggaaaatctt ccttagcctt
     8101 gatattatta ttgatggacg cagtgttttg ccttcgtggt cagctatggg tacactcaat
     8161 ttcagatatt tttccgctgt agttaatgtt gaatatatca tatattgatc ctcaaaggct
     8221 ttggttctgg tatcactggc cagctccatg cctagagatt gcagtactcc cctatccata
     8281 tcagataaaa tcccttctcc atcacccggg gcaggaaaca cgccatcatt tacgcccagt
     8341 atataaagag cttttacatc atggctcttt gagcgctcta tattgccaac caacacctgg
     8401 tcaagagcag gtggaataag acctatttta tactccccaa agcctattcc aattatattt
     8461 aagaatcttt caagtccaat tctttcatca cccagcaatt caaccgtctg gtccagcact
     8521 tccatagtaa tattccagac ctgtccatat tccccggcaa gagccagctc accctgattc
     8581 tcaaatttat ctaaatacat ttcaatcctg tcaggcactt ccagattgca tagcaaatca
     8641 aacaatgctt cacatatatc actggttgat tttcttcctg aagcatggcg gtgaaattcc
     8701 accagcggtg ctgttattct tcggcgtata tcatttattt ttatcagcaa gtcatcatat
     8761 gcctgttttt cggactcttc ccatatcata tcaggtatgt attcccagtc atcttctctc
     8821 gtccatttgt ttcctcttat tccacaggcc agaacatagt tttcaattaa gtcaatttct
     8881 tctctgggaa taccggcaag tccggtcttt aaatatccga aaactgcttc atatgaccaa
     8941 ttgtaaatga atatttcaaa tacggacatt atcatctgta tgaggggatg atgtgttata
     9001 tttctttttt tatcaatgaa atatggtata ccatactgtg taaatataac tctgataaat
     9061 ttatcatatg tatccagatt cccgcataca acagttacat ccttatatct taaaccttca
     9121 tccctgcata aacatataat atctcttgca gtatgctcaa tttccgtgta tatatttgaa
     9181 gccgtaaaaa tactaatgtc cgttgtttta ccagtgttct cctgatactt tttatatgga
     9241 aaggcataga aatatctttc caggtgctca agttcctgac tgttcttaaa cctgtggttt
     9301 tgccttttaa gaattacagg agactcaaca gttatattgt attcctcagc aattttggtc
     9361 agtttcttaa ctacattttt tgcaggataa aacacatcaa cgcaattgat cccgatattg
     9421 ccattactat tgccataacc attgcctgaa cccgatatcc catccggtcc cgtaactgaa
     9481 ccaagagcat tacctgtact tagacataat tctgcaaatt catctaccag acagtcggta
     9541 caaagacata tattaattct ggaaactttc gtcataagct tagcaattac tttatactcc
     9601 tgagatgtaa aaccggaaaa ttcatcaatc cagatttccg ctccatcaaa catagtggtt
     9661 tcatcaagtt tttcgtaaag cattgttaag tcatcatctg catctttata cttgtcaccg
     9721 atgatgtttt catactcctt ataaatcaat gccagctctt taagcttttg acctaataaa
     9781 gaatttgttc tgtttgcttc aattttctca atgtcatcta tattctcagt gttctcaata
     9841 ttgtcaatgt tcttaatatt cttagtacca tctatatcct tagtattctc tacactctta
     9901 gtgtccaatc tttcgcaggc cttttcaaga gcatccggcg ttatattata tctcttaaac
     9961 tctgcaatca tttcacacat agtattaaca aacccctgtt gttctgcaga tttagtgaat
    10021 attttcaact ggcctttcat gttttcaagt attttatgta ttatcataca cttaccggct
    10081 gagtttatcc gcacaaatgt gattccgccc acctcattga aaactctata ggccattcgt
    10141 ctgaaactta aaacttctgt ttgaattata ccgccggatc ccattgtttt tacaaggttc
    10201 ctttcagcct gcagtgaaaa ctgttcaggc accagcaata taagagaatt tgccgctccg
    10261 ctttcaatcc ttgactttat ttcattaagg caataatagc ttttgccaat gccggcccta
    10321 ccatatataa accgcaagct catatgcaaa ccctccgcaa actccgcagc gtattatttc
    10381 aagctccagt tcctgccgcc tctctgccta ttattccaaa ttcccttccc agatcctcca
    10441 gaacattaag tactttgtcc agttgttcag tagtgtggtt tgccatcagg ctcatcctta
    10501 ttcttgccag ctttttagga actgccggat acaataccgg attgacatag atatttgctt
    10561 cgtgcaaacg acggcatatc tccttaacta taaaatcatc acctattatc acagggaaaa
    10621 tagctgtctg cgaattgcca aggttaaagc caagatccaa gagattcttt cggaaataat
    10681 ttatattttc ccataatttt tctctgagtt caggttcatt ctctattaca tcaattgcag
    10741 caataagtga tgcggttacc tgtggtgtca tagcagtaga gaacatatat gtccttgaat
    10801 aaaagttcaa aagttgaacc agattactgt ttgaggctat aaatcctcca acccctccca
    10861 gtcccttgga aaatgtccct gctacaatat caacttttcc ttcaacattg caatattcgg
    10921 gtgttccttt tccatttctg ccaattactc cagtggcatg cgcctcatct accataacat
    10981 aggcaccgta atacttggca atttcaatta ttttatccag gggcgcaata tctccatcca
    11041 tggaatatac cccatcaact gctacaagct ttgtcctgta cttatcctta acccttttta
    11101 aaatatgttc aagtgaagcc aggtcattat gcttgaaaac ttttaaattc gtatttttgc
    11161 acccatctat tatacttgca tgagccagca tatccaatat ggcaatatct tcactctgaa
    11221 gaagagagag caatgtcccg gcgttggagc caaaacctga tgtgtatatg attgctgctt
    11281 cacagccctt aaattttgct attttttcct caagctcaat gtgtatatcc agcgtccctc
    11341 ccagaagcgg tacacttcct gcacctgcgc catatttatc aagtgcctct ctccctgcgg
    11401 caactacctt cggatgcctg gtcaggttga ggtaatcatt tgaagcaaga taaatcattt
    11461 ccctcacttc accagtatag ggatccagca ctctcctgat aggctcagac ccatcaaaag
    11521 caaccctttt atactgcatg tgatgttttg ctctaatatc tcccataaaa tcactaaaca
    11581 aatcagctct acccataata tcaacatcag gtacattcat aaagtccgcc atacttagct
    11641 tagttgaatc catattattc ctccatttaa aacttgctct tagttatatc ttaattattt
    11701 ctaatttata cctaacatta taccataaga aatcaatgtg taaaatagta atactgctta
    11761 actgcttaaa ctttccaatt caactaaaat ttgttaaata tgcagattct caaatatctt
    11821 ctccacttct tcctccagta gcggagcata aaataaaaag ccctggccct tatcacattt
    11881 gtattttttc aatacttcca gttgttcaat agtttcaatc ccttcagcca ctacttccag
    11941 gttaagttca tgagccagtt taattaaaga ttttagaatg acctcgtcat catttatatt
    12001 agatatattc tgtataagcg tctgatcaag ttttacagta tcaatgggca attgtttaat
    12061 ataatataga gatgagtaac cggttccaaa atcatccaat gcaatcctaa accccagatt
    12121 tttaatatgc tttagcacat taattgcagc attaatatct gtcattacag ttgtttcagt
    12181 aatttcaaat tgaatttgct catgttgaat tcctgtgata tccgccaatt ctatcatatt
    12241 ccggagcaaa tcagtatgga aaatacactt tcttgaaagg tttatcgaca ttaacacagg
    12301 gggaaatccc tttttgctcc accgcttttt ttgttcaaat gactccttta ccacccaata
    12361 actgatatct aaaatataac ctgtttgttc cgcaatgggt ataaaatacg caggaggtat
    12421 atatccctgt tctgggttca tccaccttaa taaagcttcc acacccgcta tttttccagt
    12481 attcaggtct atctgaggct ggtaatgaat agtgaactca ttattttgta tggctttacc
    12541 cagttgatta gcaatgtttg tatactgaga ttctttctcc ttcatttcct gagtataaat
    12601 acagtaattg cctcttactg tttctttaac attatacatt gcagtattag cattcttgag
    12661 taatgtttca agatcacggc catgttcagg ataaatagct atgcccatac ttgctgaaat
    12721 atagaattca tgtttatcaa taacccaggg ttcctttaaa caaagtctta ttttttctac
    12781 ttccgatatt atttcagttt tattttttac actggtcaag acggcaacaa attcatctcc
    12841 ccctaacctt gacactatat caggagattt tatgtatttc tgcagtatgc ttgaaacatg
    12901 tttcataagc ttgtctcctg aagtgtgtcc cagcaagtca ttaatatgtt ttaaattgtc
    12961 tatttctatg aacaatactg ccattattcc acctgttgaa gacttttcat taataataga
    13021 attaaaactt gatataagca taaggcggtt tggcaaccct gttaaagcat cataatatgc
    13081 catattatac attatatttt ccagctgttt tctataggta atatcagtac ctgcacataa
    13141 tattcccgat atgcttccat catagttata aagaagactg atattccata aaacatccag
    13201 taactttcca tccttgcata ctatttggct ctcataatta tataaaaatt taccttcttg
    13261 tatatcgttt aaaacagtta acatatgttt tcttttctca tgtggaataa aaatatctat
    13321 ccactttttg cctgaaaatt cattttcctt gtagcctgta atttttgtag tatattgatt
    13381 caaacgcatc attatgccat cattatcaag tacaactata atgacttgtg cattattcag
    13441 tatactttct gaaaactctt tttccattct caagtgctcc tgaagttgaa tttgttcagt
    13501 tatatccgta tgtgatcctg ctattctgac agcgcttccc tgttcgtccc atattcctac
    13561 acctctgctt aaaatccaca ggtatgatcc atctttgcag tacaatctat aagtattttt
    13621 atatattcct tttttcgaaa ttttgtaatt atttaaagtt tttaatgctc tgtccctatc
    13681 gtcagggtgc ataatagcga gtaatgaatt atgggtatta tccagttctt gtggatcata
    13741 acccaatgct tttttccact tggttgaaaa ataataaacg tcattctgaa catcccaatc
    13801 ccagatacca tcatttgaac cttctactgc cagttcatat ctttgctcgc ttattattaa
    13861 agcattcctg tgcttttcca actcattgaa ctgctgcctc aactcctctt caatggctac
    13921 caattcttca tgtgttgaac ttaattcatc atatgtctca tacgtttttt gcatggcatt
    13981 tttacataat ttgacatttc tatgaataac aataaatatt aggacagtag ttataattat
    14041 atgtaaacca tttttaatta gctcaaaata cctatatgta tttatatccg ggattagcaa
    14101 gtctaaaaca tattccagca gaaactccaa taagatgtca gtaaagccat atataagagc
    14161 aaatttaata gccgttttca aaggattaat ttccttttta aattctgaaa tatcagggat
    14221 agcataataa taattattat tattataatt attactatgt atagtactaa ttgctttacg
    14281 aaacacctta tgtaacatat accgccccta ttcctatatt cctatattcc tatactctaa
    14341 taatcatgtt ccagtttacc atatatgtca ttatatttca acgacaattt cccaatttac
    14401 ctattatttc cccagtttat ctgttatatc gacaaaattt gcagatggaa ttatcaaatg
    14461 tgaaagatat ttagatgaaa aaaggatggg cttaacccat cctttttaat tttttatcaa
    14521 ttttgtggag gactgtcccc attgattttt aattgtggag gactgtccct attgattgtc
    14581 atttcctatt gtccaggaat taatttttag ctattttcta tctgagactt tatttttaat
    14641 cttttttagt ataaactatt catgtaaatt atttattttt tttcagtcat tggagaatca
    14701 tccccaatga ctttattttt cttattacct gttccatttg tcataaccat cctgtataat
    14761 ctttgtaagc tcatcaagtc ctattgattt tacagtgttt acataactgt cccatttgtc
    14821 aagaggttct tcaccaatta taaacttggc aatcatctgc tcaacatagg tatttatgtc
    14881 gtttttcagg aagttcattc tttcagcttg ttccttagtg aaacgtacag tgtttggata
    14941 tactggtatt gtgtatggga agtattgttt ttcactttca gtagctttaa gtaacaataa
    15001 tgggtcggta tttggaactg catcagttac aacccatcca aatccaccag gcattacatg
    15061 cttacttata tattctacag gatttaaacc ttctacttta gattctctag tataagtttt
    15121 tcttgatgca tcatcgaaat accagttata gtcaagtatt ccaaggaagt tactcaatcc
    15181 gcaaattcct tctacgtttt catcaacttt tctgaagaat acatctgccc acctaatcat
    15241 agcttccgga tatttacatt tatttgttaa tgcaaatgaa ccagtgaaaa ctccaggata
    15301 ctctgctgtt actttcttgt tgttacttgc ggaagttaat ggtgaaatta actcatatgg
    15361 agcagattga ccggggttta atgatgaaga tagtgagatt gcacttatgc ccatagtccc
    15421 ttccttatac ttggctatat attgctgcat agtttgagta aacatatcat ggtcgagtaa
    15481 attctctgaa taaagccttc tcatatatgc aaggtagtct ttgaaggcag gttgaacagg
    15541 gttaaataca actttattgc caacaagatc aaagcctgct ccaccggcct gaccactgaa
    15601 ggcagcatag aatattggat gcaatattcc accgatactt ccagcaccgg cagggttctg
    15661 tttccaatag gataatggta tttcatcatt cttgctattt ccatttggat ccttttcttt
    15721 gaaagctttg agcacatcat agaactggtc tacagttgta ggtttgctca tcccaacatt
    15781 tgctagccat tccatattaa tgtacaaaat atttgctgcc actgtcttag ttctcactat
    15841 ataaggtaaa gaataaatat gtccatcagt tgctgttatt gctttcctta tatcgggatt
    15901 ttcagtaagt atctttttaa tataaggtcc atatttatca atgtacttat ccagctgtaa
    15961 aaataccccc tgaggtccat aagtttcttc atcctgtatg gttattccgc cttgatagaa
    16021 tacatcagga taatcaccac ttgcaaatgt caggctcttt ttttccggat aacctgttgc
    16081 taatggagta tcaaagtcaa agcttatatt tgtaagcttt tccattctct taaatacttc
    16141 catgtctttc cattcgccat ggagcggact ctttgatccc ataagcttta atgcaacttt
    16201 ctcttttgta atagggaatc cagtagcgtt aatgtaagca ggaatatcat ctttctttgc
    16261 agttgcttct tgattcttct gcgtttgttc ttttgtagct tcctctgccg tagtagttgt
    16321 gatctctttt gagctagttg ctttttctat aacaacacac ccagccatgc ttcctgcaac
    16381 catcgcaagg attacaacga ttggcagcag atttttcaat gctttgaact ttttcattaa
    16441 ctaataacct ccttaaaaat tttttactaa tattatcttt tttgtaaaaa cctgttgtcg
    16501 tttatccttt aatagcacct accataatac cctgtacaaa atatttttgt atgaatggat
    16561 atgctataag tacaggggcg gtagatacta ttatgaccgc atatttaatc atatctgcaa
    16621 ttttagcctg cttggcaatt gcttcgattt cttcaccggt tttcatcatc tcggtgctca
    16681 tttcatttag aataagaatt tctctcaata taagctgcaa aggaaacttt tctctgtctg
    16741 atatatacat caatgctaga aaatagctgt tccagtgact taccccgtaa aacaaggcaa
    16801 taactgcaat aattggagta gaaagaggca aaacgattgt aaggaataac cttgtatttg
    16861 aacagccatc tatttccgct gcttctttca tttcatttgg tatatttgat tggaaatatg
    16921 tccgtgttat tataatatta tacatacttg cagctcctgg tagtaataat gcccagattg
    16981 tatccctcat gccaaggcct ttaataataa ggtatgtggg aataataccg ccactgaaaa
    17041 acattgtaaa ggtaaaaagg cccatgaaaa gatttcttcc atagaaatcc tttcttgata
    17101 aagaataagc tgcagggaga gtcacaataa tatttactat agtaccaaaa aaagtataaa
    17161 aaatggtatt tctataacct gtccatattt ccttatcttt gaatactctc agatacccgt
    17221 caaaacttat gccttttggc agaagcaaaa ctttgccttg atttaccagg tctgggtcgc
    17281 ttatagaggc actaagtaca aatatcaatg gatatatttc tactatcagg acaagggtca
    17341 taataataac atttattata ttaaaagctt tatcagattt tgattcttta attacactca
    17401 tatcatgttc ttcctccttg tataatagtt cctgtttctg tacacagccg caaagctggt
    17461 atcagggact cattacttta ttccgtacca attgtatata ttgtacctat taccacagac
    17521 ttgtttcatt cagctttttc gcaatattgt taactattat aagtaaagta aaattaatta
    17581 ccgagttaaa aaaccctact gccgaagaaa agctgtattg agcattcaat aagcccatcc
    17641 tatatacata tgttgatatt acatcagaag tttccatatt taacgaattc tgcatgagga
    17701 ataccttttc aaatcctaca ttcataatgc ttccactgtt aagaatcagt ataataacca
    17761 tcgttggcat tattccaggt atattaatat gccttatacg ttgaagcctc gttgctccat
    17821 ctactattgc agcttcatgc agttgagtat cgattccact taaagctgca aggtatataa
    17881 ttgagctcca tccggcactt tgccaaactc cagtccaaac atatatactt ctaaacatgc
    17941 tgggcttaga cataaaagga atcggttctc ctccaagagc ttttattatg ccattaataa
    18001 taccattgga aggtgcaaga aatgcaagga tcatacctac cattacaact gttgagataa
    18061 agtgtggggc ataagttaca ttttgtacaa gtttcttaaa tttctcattc ttcaattcat
    18121 taagtagtaa tgccagtatt ataggtatgg gaaaacctgc agccatggaa tataaagcaa
    18181 ttcctacagt attttttatc aaaagattaa aataataaga tttgaaaaaa tagttaaagt
    18241 gtttcatacc tgtccaggga cttccccata ttcctttggt agcaataaaa tccttaaatg
    18301 ctatctgtat accatacata gggccataat gaaatattag gaagtataat aatgccggta
    18361 atataaatat atataattca tagtttttaa ttactttttt ccatacatta ttcccttttt
    18421 ttataggttt tattattgca tttgcatcaa gttttgtatc aagacttgca actgtatttg
    18481 tgttttgcat catatcttaa gctcagctcc ctttttatac agctttctgg tttatgttgt
    18541 ttgtttttct gaagaggtaa tcacattgac tcacaagtaa agaatatcat tgaaatataa
    18601 aagtgtaaat tagtttttgt ggaagatatt tgtttttgtt gcatatagat tataattatt
    18661 aaatagaaaa tagagcgtta aggagaataa aagagatttt aagggattaa tcaatatttt
    18721 ttatacaaaa gcacttcacc tcttcaacta cctattggtt ttctttgcag ttgtttgttt
    18781 ttcttgcaga atacagttcc ctgtactgac caggagtaat tccttcagtt ttcttaaatt
    18841 tcttcatgaa atatgtcaaa tcagtatatc caatctcctg aacaatgctt tttattggca
    18901 tatcagtagt acttaaaagt tctcttgctt tttccatttt caaatttata acataatcgg
    18961 taaatgtata atttgtttgt tctttgaaaa atctgcttaa gtaataaatt gaaaagccga
    19021 actcatcagc tacactttcc aggcttagat taacatcatc aaagtttttg ttaatatatt
    19081 ccagtatctg gttgtttaat tcttcgcttt tagtattctt tctttctttt atataatcgc
    19141 acaactttgt aacaattatt ttagtttcct tttccagttc ttctattgtt gtgaacttca
    19201 ttaattcttc tatatcataa tctacattgg ttattttcat ttcattaata gtctttatta
    19261 tcatatttac tatatcatag taaatgtact ttatcattaa gacagaagac tggtttttag
    19321 ccacattgtt aagcattatc tccagtgctt caatagccac agctttatcg ccttgtttca
    19381 agctctgcac aaatcgcagt tggtcttcta atggatacca aaattgctgc tgctgagatt
    19441 ttacaatatc attaaacatt aatatgccct gtctgcttct gactttattc tcttccagag
    19501 cagcataagc ttcaataaag gaacggttta ttttattcat attattatat agtttgcctc
    19561 cgccaataac aacattttcc tcggttgcat cttccaacaa ctgctttatc ttataggtaa
    19621 actgcaggta atcttcatcc tgatcctgac tgatatttag gttaaggatt aaagctatag
    19681 aatcatcttg aaataattct acaggatata cataaccatc tgcgttgaac ttttcttcta
    19741 taacctcaaa caccttatct ttaaacaccc tggtatgcct ttcttcagtt tttattcttt
    19801 tatccactat tgccacagca aaagaaaatc cagttaattt aatattggaa aacacaataa
    19861 gactcttcat ggttccttca ttaagtactt caccttttaa aagctttgtc aggatctgct
    19921 ctcttataaa tggtctctgc atatttatct gtgcttgcaa gctcttgttc tgcattattg
    19981 cagtttcaac agtgtgacca attctttcta tctcatccct tctcttattt ctattactca
    20041 tgttcttatt cttattactc ttattcctat tacttttatt ccacatgctc ctattccact
    20101 tgaaacctct tataatatca agacaattat tatagttatt attagaataa ttatttgaat
    20161 cattactgga ataattatta gaatcatata ataaatcaga tgtttcatca agtgatacat
    20221 tatatgattc atttggttcc ggccattgaa gtgtaataaa attcataagt tttccgatag
    20281 gtttgtaaag tttggttgaa aaggcaactg ctaatatagc tccgaatgca agtattatta
    20341 aaaacgcctc aagtattagt gtcttaacat ttattaactg ctgaaaaaac tgtgctccag
    20401 gtattgcagc tacaaattta aggcctgtat tttctgaagt ttgaattatt aaagaaacat
    20461 ttttatttcc aaactttgca tccattatgc cgtttttctc agtagtcctt ataaagtcta
    20521 tatcttccga tgccaattta aatgatttat tttcagataa taatactgaa ttgtttttat
    20581 ccagaattat catacttcct tttatattac caataaaatc cttaaacaaa gctcctatag
    20641 ttgacttctt tataaggaaa ataattgctc cataagggtc atagttgcca taggaaacag
    20701 ggtaaatata agttattagt tctacatttt gggaattagc caggctaagt ttttcttctc
    20761 ctataaattt ggagaatttt aatgcttcta tttcattaat aaagttttgt tctgcttttt
    20821 tatcatattt atagacattt cttgcaagag tagataaagt actttgacca cttgcagaat
    20881 atacattacc tgtacatttc aagtacatat aataatcgtc aataaaggca ttttgcgctt
    20941 tatacattcc caactgataa aggccttcta atcttttata ttcgttgctg ctcatattat
    21001 taggggttag tttattgtca acatttattc tcaaagcaag gtcttccagt tccttaatcc
    21061 ttaaatccaa attattcttt aactgggcta acttatattt attagaagct tcaatctctt
    21121 tccgtatatt tacaactgaa atgttatata acaaacctgt cattataatt agaggggtag
    21181 ctacaataag gaaatatgaa attaagagct tatatttata taatgtgtct gtcttcacaa
    21241 ttttcccaag cataataaca acctccgatt taagagagat gcatcattcc ttaatacaat
    21301 tggacattcc tcaacattaa aagaatacct cctacttgtg agaggtgtgt cccttctcta
    21361 attatataag tatgcatctt aaaaggcaag ccgttttttt gtgggagcat acaactcatc
    21421 tattcactca gttctcccca ctccatataa agagaatcaa ggttaagatt aagttcatcc
    21481 ttttcttcca gaagagtttg aaggctttcg tagtcacttg ccctctgctc catttctatt
    21541 tcaatatcct taagccgttc ctcaagaatc aatatatctc tttcaagctc acgcaatctt
    21601 ccggcaacct gtctttctct cttggacttc caccccttgt tttcaatggc tttgttatct
    21661 ttattttttt tattcttttc tgtattgtta ttcttttctg cattgttatt ctttttagca
    21721 ttgttattct tttcggcatt gatattcttt ttagccttga taattactgt ctctgtagtt
    21781 ttattattat ttaccgtttt ccactttctg tatgattcat atgtgcctct aaagtcagat
    21841 atgctcccat cttccatgtc ccatatcctt gtggcaaagc ggtttacaag atatctatca
    21901 tgggaaacaa acaacaatgt tccatcatat ccatcaatag cttcctctat ccattccctt
    21961 gagacaatat caagatggtt tgtaggttca tcaagaatca gaaggtttat atcgctattc
    22021 attagaatac atagttttaa tctgcttttt tcacctccgg acaaagtgct tacctgcttg
    22081 aacacttcct cacctttaaa atgatatgct gccagacggt ttcttgcagc ttcttcactc
    22141 acaataaggg cattcctcac agtatcaaga acagttagtt caggattatc aaatacaaca
    22201 atttgaggaa ggaatgcata ctttatgctt gtcccaatcc ttgaaaaacc tgaatcagga
    22261 gtctcttccc ctgttattag gcgaagtaat gttgttttac cgcatccatt attgccaata
    22321 agacctatcc tgtctccttt ttctacagta aggtttatat catctataat acatctggca
    22381 ttaaatgatt tgcgcagtcc ctttataaca ataacatcat tacctgcaaa ccttatcccc
    22441 ttgaatacgg atttgatccc tttttctctt gttggcctat ctgtcctttc cattttttca
    22501 attctttttt caatagcaaa agctctcctg tgcatggagg ggttatccgc tacacttgcc
    22561 cattgatgca tttttcttgc agcttcttca agctgcctga tttttctctg ttcctgctca
    22621 tattgctgga gtttctgcaa gtatctggat tccttttctt ttacataata actgtagttt
    22681 ccttcataaa actccgcttt tccatctaca atttcaacaa ttctctgtac tactctgtca
    22741 aggaaatatc tgtcgtggga tattgccaaa accgtacctt tatattgaga aagataatcc
    22801 tccagccatt ccgtcatatt aatatccaga tgatttgtgg gctcatcaag aagaagtatt
    22861 acagggttct gcaatataat tcttccaaga ttaatccttg tcttttcacc gccgctcaaa
    22921 tggataaata atctatcctg catatccttg tcaataccta acccgttgca gatttttgca
    22981 atatcactat caatggtgta accacccata gcttcaaatt cagtctgcag aacaccgtat
    23041 ctttttacaa ttgatggttg tgaagataaa gctgaagaaa cagctaaaga agtattcgaa
    23101 gcagcattcg aagtagcatt cgaagaaaca gccatcaatt tctcaagttc cttcatctca
    23161 ttcttaatat ttgatagtct ttcaaaggat gtcttcaata catccaacac tgtataatca
    23221 tgtggatatt caggtatctg gtcaagtata ccggctttac ccgataaatt tacagtacca
    23281 tcatcatatg cttccacccc cgacaatatt ttgaataaag tcgtcttccc tgctccgttt
    23341 ttcccaagca agccaacttt ttcaccctta tacacctcaa aagtcatgcc ttttaaaatg
    23401 tgctccgatc cataatattt atgcacttcc cgcacagcaa tatccaaagc aaccactcca
    23461 atcaaaatcg cgtccaatat gtttgaaacg catttgaaac acactaataa aaggatatca
    23521 atgaatcagt agatatgcaa ctataagtta ttatatgtgt gcatttactg aatttgatga
    23581 agtatttacc aggggctgtc gttacagtgc ttagagcctt taaattcagc tacaggcaaa
    23641 atcaaactca caaaataatc tgaataaaag aattgtaatt attttgttca aacactaatt
    23701 ttgctcttcc ttcgctcaga tactgagaaa ctcttcagca taaaaggaat acctgttact
    23761 tgagagaaga gttacccctg cctagctaca gaactttcac ttatgccact taccaatatt
    23821 ttcttcaaat aaattaaatg cacccttaat ataccgtatt taataatatg cagaaaattc
    23881 atacttgaat tttttattca ttttcagttt gacaaagaat taacaatgtt ctattcttaa
    23941 aatataacat aatgataact aaaaaagcaa agactattcc atagtttata actattttgt
    24001 agttcataac tattttatag tttataactg ttttgtagtt cataactatt ttgtagttta
    24061 taactatttt gtagtttata actattttgt agtttataac tgccttgtag tttattacta
    24121 tcctatagcc tgaaatatat taatctggaa catattaatt ataatattta atattggagg
    24181 taaaattatg tgggaacaaa atattaacat taatgaagta agagaaatta gatgtaagac
    24241 tactgcttac cttggagcag gagccatgga aaaaatgaat gatatcgcaa aagagctaaa
    24301 aaaaatgggc gcagacaaga ttattgcagt taccggcaag tcttcatata agaaaaccgg
    24361 tgcatgggat tatgtggaga aagcatgtct tgaaaatgcg atcgactgga ttatattcaa
    24421 caaaatttca cccaacccat catcaattga tgttgatgaa gcaacaaaaa tggctattga
    24481 ctttggagca aaagctgtta ttggtatagg gggaggaagc cctatagatg ctgccaagag
    24541 tgttgctata atggtaaact atcctcaata tacaacagcc gacctttatg aatataagtt
    24601 tactcctgag aaagctctgc ccctgattgc agtaaataca acacatggta ccgggacaga
    24661 ggttgacaga tttgcagttg taagtattct tgataagcag tataaacccg ctattgcata
    24721 tgatataagt taccctctat atgccataga tgatcctaaa cttatgttag gtctgcctaa
    24781 aagccagact atttatacat ctgtagatgc tgtaaatcat attgttgaag catgtaccag
    24841 tgtagtagca agcccatata cagtactttt agctaaagaa acagtacgtc tgatagagaa
    24901 gtatctgcct aaagcccttg aaaatggtca ggatcttgaa gcaagatatt atctgacata
    24961 tgcatcaatg attgcaggta tctgctttga taacggtatg cttcacctaa ctcatgctct
    25021 tgagcatccc ttaagcgcag tcaagcctga tctggctcat ggtcttggat tggctatcct
    25081 gcttcccgca gtagtaaaga acatatatcc tgcatgtggg gaaattcttt gcgatgttct
    25141 ggctcctata gtaccaggtc tcagtgggca agcttcagaa gctgaaaagg ccagcaaggg
    25201 agttaaaaaa tggctggcaa acgttggaat tacagagact ctatcaactc ttggttttac
    25261 aaaagctgat gtagataagc ttactcatct tgcgtttaca acaccaagcc ttggacttct
    25321 tttgagctta tcacctgtaa aagcaacaga agaactggta gcaggaattt attcagaatc
    25381 actataagta ttatcgcgag tattattgtg agctaatcaa gagtagcgtt atgtgtataa
    25441 tataggttaa tctcacaatt tgatataaat tgagcatata ttcattggat ggctataatt
    25501 ttaaaacctt gtgccctttt tggatgcaag gttttttcta atcctaattt aatatttctt
    25561 catatttgat agatgcaccc aataatcaat cagtcatttg acatgttttg gcaacactat
    25621 tcagctactt tgtatgaata aactacttac cattagtaaa taattactgc tgaatagctt
    25681 tttgttatat taatataata ttataaagta tataaggcaa aatggagggg ttctttgaca
    25741 ggtttctata atgaaaaaag atgggtaaag ctatctttag cacttatcat tgcacttttt
    25801 actataataa atgtatattc agtactcaga tataatgatt attttctgct gggaagtatt
    25861 gagaaggctg ataatgacga tgtgaagtat ataggaagtg cttggacact tttagaaaag
    25921 ggtatgctta catatcacga tgttaacgtc cctactgttt acataatgcc aggccatacc
    25981 attataatcg cagccattac gggaatattc ggaaaatatg gcgggattac agctttcagg
    26041 ctgtttcaag ttctgcttca agcattgtcc atacttttag tatttttaat cggcagaaca
    26101 gtttttaaca gttcagtagg tattatagca gcatttataa atgcaacata cgtttctgaa
    26161 atatttgtta ccgggacaat acttactgag gttgaattca aattcctgct tctattgctt
    26221 gtatatatat caattaatgc aataaagaca aaaaaaacaa ggtattatat agaaggtggt
    26281 atagtgtggg gtattgcatg ccttttcaga cctacaattg cattataccc agttgcaata
    26341 ctggcagcat ggcttatata caaatactct ttcaaagaaa tatttaaatt cagcatcata
    26401 gtcttggtga tttttgctgc tattatgtct ccatggtgga tcagaaacta cattgtcttc
    26461 aatagattta ttcctcttac cctctcatcc ggtaatccat ttttgcaggg gacatacata
    26521 aattatgacc aatccaagga ttatacacca tacaccccta gcaaggatgt aatagaaact
    26581 aatgaaattg aaatgaaaac aggaatatat cgattgaaaa catattttgt aaaatatccc
    26641 ctaaaatata tatattggta cacttttggg aaatcatggt acttatggaa ctaccctttt
    26701 tactggaagg aaatcttcgg tatatctgta acaatggcaa cagtgcacca tcatttaatt
    26761 ctaattgcag gtataccagg aattatttca atttttggta acaagagcct tgaagggata
    26821 tttttaataa cggtaatatt gtacttttct ctttcatact taccctttta cacattttca
    26881 aggtactctt atcctatgat gccgctagta atgctttttt catcatatgc agtattcaaa
    26941 ctgtataaag gaatatccgc aatgtgcagg actgtcccct gaatgtacag gactgtcccc
    27001 gaatgtacag gactgtcccc gactaagaat tagtcctatt cagaattaat cagaattgaa
    27061 gaactgtccc cgtctggaaa ttaaccatag ggccttcata taattttgaa agacctggta
    27121 tgcttttaaa attatcgggt actacaagag atgcatcttt gttgaaagta tatatgctcg
    27181 ctgttgataa caatgtagct gcaaattccg aacatgtata tctgtttttc atctttaaag
    27241 gtttattaat catgatccct aagagagctg caaaattata atgatattta tagcggttat
    27301 taccatagca cttaataagt tcaacaatca cattatattt ttcctcgcta acatccagag
    27361 aatatatgca acaagatgca tttttaaaat ataggtaaac ccctttatta agctcctctc
    27421 ttatgaatcc tccaattaaa gggaatcgta tataccttct tccaaagcta tagaaatatt
    27481 ccattctttc atcgacacat atagaaacat ggttgtattt cgcatgcgta taaaatctta
    27541 tagttttcat aactaaagaa tctgtatgag caagcattat atatatttta ggcaaatatt
    27601 caacctccgt gaattcagaa tttataaatg ttacaaaaag ccatgtgagt acagttgtga
    27661 atacagttgt gggagagttc aaattgattt actattgcct ctaaagcgtt taacaagatt
    27721 tttcagcaca ttgtaatttt caaaatagct catattatag gttacctcgt tctctctggg
    27781 cactgtaaga atttcaagat ggttgattcc atcaggatag catttgtatt catacgtcct
    27841 ctcaattcca tcaacatcaa gtagctttac ccatataaaa gttgggaatg ttctcaatgc
    27901 ttcagttata ccttcttcag tctgtacatc ccgactgttt atactcaata tgatatcacc
    27961 tctcctgatc ccaattctcc aggcatgact gtcaggaaga acatctagta ctctgactcc
    28021 tctgcgtaca ggtataaaca aaggtgaatt attcatttca cgataacggc catataagaa
    28081 aataaactcg tgtcctccca gagcaaacag agcacccata accttaaata atgaaatatt
    28141 tctggaaata acagccagga taaaaagcaa tatactatat ataaggagcc atatagctgt
    28201 ttcacggctt ttattttccg gcttccttgc tatggctata tcagtataac acaaagctga
    28261 aactgcacag tccaatccta ttgctaaagc acctgcatgt acagcactag agttaaataa
    28321 tgtccaccag tcaactttta tggtgtttat gctatttaac gttacatctc caaacctttg
    28381 agccagaaag gtaagaaata taacaggtac aggccagatt cttctggtta taaatgcgcc
    28441 ggcaatactc tgtccatgct taatgtaaac aggctcacaa tccctgcctg ctccggcaaa
    28501 taccaatata ctttcaaaaa agtgcattac tgcaaccaat gccagcaatg atgctacatc
    28561 aatatttgaa attttaaagg ccactgctat taaagcaagt agagcagctg aataagatat
    28621 acaaataaat ctaatattaa acaatattaa tactgccata ataattaata ggtactcaaa
    28681 tacttcaggt gcaagtgtta tgcctgtaga aactactatt acaccgccaa agaaacctat
    28741 tattgaccca taaaacatca tttcttttat agtattccat atccctctgg atggcactcc
    28801 cagccaatta gtgactatgc ccatttgttt ttcatgttgc attttaataa gcaatacaac
    28861 aattacataa ataggtataa atctataatt aataataaat gctgacagta attctcttgc
    28921 tactgccgat agaatatata ttaaaggcaa atttattcca ctcctactcc tgctgcaata
    28981 tacatattca aattatttca ctcaaataag taataaatct ttatttgtat actattgttc
    29041 tcaaaaagtt aacaaaaaaa caaactttta tttgcaaagc cctgtaacgc aagcgttgaa
    29101 gagcattttt cgagaacata taaaacctaa attaatcgca acacacgctg ggtacataca
    29161 ttatttaaaa atgcaccccc acacgcttgt atgtttgcag ctatacacta cgatatgcta
    29221 taattatata acatattttt atttaaacaa ggggagatat ctttgtcaga ctttattata
    29281 aattttatta aagaaagtcc atatatactt gctgatgggg caatgggaac atattttgcc
    29341 gaaaccagcg gaataagtgc caaaagatgc gaattcgcaa atattgaagc accagaaata
    29401 ataaaaagga tacatgagga atatattgct gccggttctc agattttaag gacaaacact
    29461 tttgcagcaa acacaatttc tttaaaaaca gattttgaaa ctataaaaga aattttaata
    29521 aagggatttg aaatagccag ctctgctgcg aagaataaag gtgtgcatgc aattgcggat
    29581 attggtccaa tacctgaagc tgaaggccat gatactcttc aggagtataa gaatattgtt
    29641 gatattttct ttgagtgtgg agcaaaaagt tttatttttg aaactttcag ttcttatgaa
    29701 aagcttattg aagtttgcaa atatattaaa agaagggata gcacaacctt tattcttacc
    29761 caatttgcag taagtcctga tggttatacc cgtaagggga tttcagggaa gagaatcctg
    29821 gcagctgtat gcaattccga tgtaatagat tcatatggat tcaactgccg ttcaggcccc
    29881 atgcatcttt tagaatatgt gcgtaatctt aatattcctg cgaaacatct tgaaatgttg
    29941 tcaataatgc ccaatgcagg gtacccgact actgaaaatg aacgtacact tttcttaaac
    30001 aatcctgagt actttgcaca cataatggct gatataaaga atcttggtgt aaagattttg
    30061 gggggctgct gcggaactac tccacgccat atattggaaa cagcaaaagc cctcggcaac
    30121 cgcagagaaa cctctatccg tgttgatgca atagcaaaac aagagtcaac ccgggtaaaa
    30181 gtcaaaaaca gctttcgtga gaagcttgac ttgggtgaat taactatagc tgtagagctt
    30241 gatcctccta taaatgcaaa tataactaat atgatagagg gagcaaaagc cttgggggaa
    30301 tgtggagcag atataataac aattgccgat tgtcctcttt ccagagcaag agttgacagt
    30361 tctattcttg catccaggat aaaacgtgaa acaaacatgg aaactctccc gcatttaacc
    30421 tgcagagaca gaaacttaaa tgcaataaaa gcattactgt taggtctgca tatagaagat
    30481 atacgaaatg tgctggcagt taccggtgat ccaattcctg aagctgaaag aggcgatgtt
    30541 aaaggagtct ttaacttaaa ctcttatatg ctcactgaat ttataagaga actgaatcac
    30601 actgagtttg ccaacgatga aatttttatt ggtggagctc ttaatgtaaa ttctgtaaat
    30661 tttgatgttg aattgaaaag ggctgaaacc aaggtcaaca agggaactga attttttcta
    30721 actcagccgg ttttcggtca agaagcggtt agtgctttga ttaaagcaaa aaacaccctt
    30781 aatgcaaaaa ttctcggtgg tattcttcct attgtaagtc accgtaatgc aattttcctg
    30841 aacaacgaaa tacctggcat taaagttcct aaagatatta taaggcaatt tgagggaaag
    30901 gagcgagatg aagctgaatc tacaggtatt aatatagcta ttgaaacatg caaaaaaatc
    30961 ttaccctatg ttgatgggtt ctatattata acacctttta acaggtggag gatggtatgt
    31021 gccattatta aaaaaatccg caatgcttag tactactgtg ccaatttgga aagcgttgtc
    31081 ttccgggata attttgatag tatcgggaag atatcgcata gggaaatgtc tctatagaag
    31141 gcttgcgacc cttccctcag atatattcct tacgagccag caaaaattac cctccagaaa
    31201 tgcgctttcc attggcaaca taacactgaa ttcataactt tataattatt tccttaataa
    31261 gtttcacaaa atcactggtt gcccggtttg ctacctgtac tacatccctg tgactcaggg
    31321 gtttattaag tacaccggca gccatgttgg ttatgcagga tatgccaaga acactgattc
    31381 ctccatggtt ggctgcaata acttcaggaa ctgttgacat gcccaccgca tctgccccta
    31441 ttgcccttaa ataacgaatc tctgcaggag tttcatagct gggtccggtc atagcagcat
    31501 atacaccctt acgaagcctt atagagagcc ttttggcagc tttctccgct ttttccatat
    31561 gttccgggtc ataagcttta gtcatgtccg ggaaccttgg cccaaagctt tcactattca
    31621 tactaattaa gggatttaca cccatcaggt ttatatggtc ttctataagc attaagtctc
    31681 ctggactaaa cgacgtatta attcccccgg cggaattggt cacaattagt tttgttatcc
    31741 ccagtttttg taataccctt ataggataaa caacttcatg aatactatag ccctcataga
    31801 agtgaaacct tccctgcatg caaacaacgt gctttccgga caatgctcca agaataagct
    31861 gtcctttatg accttccact gtagataccg gaaaatgagg aatactttta tatggtatag
    31921 tagtctgatt ttctatatct tcagccagca atccaaggcc tgaacctaaa attataccaa
    31981 tttccggcct gtttacattt atactattta tgttttttat atcttttata tcttgtatgt
    32041 catgcatgcc ttcgatatct tgcatgcttt gtgtgtcttg cgtgttttgt atgttttgta
    32101 tgtcttgtgt gttttgtgtg tcttgcgtgt tttgtgtgtt ttgtatatta tttgcattct
    32161 gtatatcttg tatatacttc aaggaatcac taattttctt agataatagt ctcatttatt
    32221 gcctccagta tatttaacta tcttggtaaa agtgcattta ttccaaaagc actgaaagca
    32281 cactcgtact gattgctgaa agctagtagt cagttgtcgt ccaagggtgc acataattgt
    32341 agtatatccg gtatatacct tatctattcc gttgcatagt tatcaaaata gccttgaatc
    32401 aatactattg gagtaccttt atcaccactt ccgctcataa ggtcacataa gcttcctaaa
    32461 agatcggtaa gatttcttgg ggtcgtcccc aaagattccg ccttaccaac catattattt
    32521 cccttttctt ttatttttct ctttacggcc tcatctattt cgcttttgtt cagactatta
    32581 aattcattat ctgctacata ttttaactta atttcatttg ttgtcccaca aagtccatct
    32641 gtgtatcctg gtgatacaac cggatctgcc agttcccata ttttccctac cggatcttta
    32701 aacgccccat ccccatatac cattacttca atatgctttc cggtcttttc aattagttta
    32761 ttttgtattt ctatcactgt tctattgcag tctctaggaa ataactttac cttatcttct
    32821 gtggacatat ttgaaccaag aagtccaaaa tcaggattaa acccactgtc attaacggat
    32881 tcatttaata tttcatccaa accatatact ttcttagcac ctgaatttaa caatattctt
    32941 ttagttctgc tgcgttcatg aatgttagct actaatattt cgctgctgta attcagtgct
    33001 gcctttggat cattcgtaag atatatatct atattaccat tctgggcaat ttctttgtaa
    33061 gccttaatat aatctattcc cgtaaaagga tgtactacct tttctccaaa aagctctcgg
    33121 aattttttct cactcaatgt atcagtatag ggatttatcc caagctcatc cattttatct
    33181 atatcaataa gtctgttccc tacttcatca gatggatagt ttaaaaacaa gtatattttt
    33241 ttacctgtca ttgcaatacc cttaagaatt attgaaaatc tatttctgct taatattgga
    33301 aacaccacag caatatcgcc attaaatttt ctatttatat caaaagcaat atcgtttatg
    33361 ctggcataat taccctgtgc tcttgcaacc agagattctg taacccctat gacatctctg
    33421 tcattaagat ggtaattttc ttcctgtgca gattttaaaa ttgaatcaac tacaatattt
    33481 acgaggtcat cccctgattt tattattgga gctcttattc cccttgctac cgtacctacc
    33541 gttcttgcca taaaattcct ccttgttact ccttacatct gaaacttttc aagctttaaa
    33601 gtattaaata caaaataatt tatcatatac tattatgcac ttaactccca gaataagtat
    33661 acaacaagga caacaatacg tcaaaagttt ttttcagctt ttagcagctt cggattgtcg
    33721 gtttataggt ctgtctgttt atctgtttat ccaggcagaa atatcctcca ttacctcgtc
    33781 cctgttaatt tcatttaaaa tttcatgtct tgcaccctga taaagcttta tttcaatatt
    33841 tctcatacct atacttttat agttttcata aagctttttt actccagaac catatttacc
    33901 tacaggatcc tgatcacccg aaataatcaa taaaggtagt tcataaggaa tgctgcctaa
    33961 acgctcatcg caataaagct ttgccaggcc ttcgaaaaag taataataga atcctgctgt
    34021 aaaaaccttg ccacagtaag gatcttcatc atactttttt acttcagctt catcacggct
    34081 aagccagcta aaaggtgaaa ccggattttt tatcattttg ttataactgc caaagcttaa
    34141 tttgttaagt aagtaagcct tctttctatc tccgaaaaca tttctggata ttgctgctat
    34201 tattttgcct accgcaactt cagaaccttt agtcatggaa gaacctgaca aaattgttcc
    34261 ggaaatactt tccccatatt ttattatata ctcttgagct acaaaagaac caaaactatg
    34321 tccgagaatt attatcggaa tatccgggtg aatttcttta attttttttg taattatata
    34381 ttcatcctct acaatcctgt taaacccatc ttcacctata tagcctacct cttctaattt
    34441 gcctgcagtt ttgccatgac ctctgtgatc attggcatat actgcaaaac ccttgctatt
    34501 catataagaa gcaaacctcc catatctgcc ggcatgttcc gccatgccat ggaacacctg
    34561 tattacagcc cggggtttac aacaatcgtt ccaggaataa acataaatat ctttgctggc
    34621 ataaccattt atatagaatc cattttccat acaaaattcc ctccatttag tactatttga
    34681 acacacagca tttggcataa cctatgcata tattgcctaa acatcccatt ttaccgacct
    34741 ctctacagca ttcttccatc ttaaatattt gttcatcctg atttcatcac tcattgccgg
    34801 agtaaatacc ttatcaaggg cccacttatc ggcaatctct tctttactgt gccacaggcc
    34861 tgctccaagg ccagccagat atgcagctcc cagagccgta gtttctgtta tttgaggacg
    34921 cttaacatta atgctaagaa tgtttgcctg gaactgcatc aagaaatcac ttacgcttgc
    34981 ccctccatca actttcaatt cctttaattc tatccccgaa tctattttca ttgcttcaaa
    35041 aacatccatg ctctgatata ctattgattc aagaacagct ctaattaaat gctgtcgctt
    35101 tgtacctctt gttattccta caatagttcc tctggcatac atatcccaat agggtgcacc
    35161 cagacctgta aaagctggca ccatatatac tccacccgta tcagctactt tttccgcttc
    35221 gatatccgat tcatgagcag tttttatcag cccaatttca tccctaagcc attgaattgc
    35281 cgaaccagca ttaaacacac ttccttcaag ggcatattca acttcattac ctataaccca
    35341 ggctatagta tttagaagat tatgctctga ttttacaggc tgtttcccta tattcataag
    35401 aataaaacat cctgtcccgt aagtgttttt tgcagtcccc acatcaaaac atgcctgccc
    35461 gaataacgca gcctgctggt ctccggctat gccgcttata ggtatgggga ttcctaatat
    35521 ttcttgagaa gtgtccccta ttataccgga tgatggtact acagttggaa gcatgcattc
    35581 aggtattccc atctgtttta aaagctcctt atcccattga agtttcctta tattgtaaat
    35641 cattgttctg gaagcatttg aatagtccgt tacatgtagc tttccacctg taagattcca
    35701 tataagccag ctgtccatgg tgcctgctaa tagttcacct ttaccagccc tttccatggc
    35761 tcccggaaca ttttcaagaa tccattgtat ttttgttccg gagaaatagg catctattac
    35821 aagacctgtt ttttctttta ttacatcctg caggccatca aactttaatc tattgcaaag
    35881 gtcggcagtt cttctgcact gccatacaat agcattatat acaggtttcc ctgtaatttt
    35941 atcccataca actacagtct ccctttgatt tgtaatacct attcctgcaa tttcatcagg
    36001 ggatatcccc gattcttcaa caacactttt taaaacttca agctgccctg tccatatttc
    36061 caccggatcg tgttcaaccc atccgggatg aggataaatc tgcttaaagg gtttgttttt
    36121 aatattcttt atggtcacag tttccatatc aaaaagcaca gcccttgagc ttgtggtacc
    36181 ctgatcaatt gttaagatat acttcttttt cattgaaata ccccctggag atctatagaa
    36241 aatttattat ttgccggaca gattacttag cattaattgt acaataattg ctgtttaata
    36301 attactgttc gataattgct gttcaataat tactgtttaa taattactgt ttaatatatg
    36361 ctactaatat ttattatttt atatcagttt agtgcaaata acaacaatat cctgaatata
    36421 caagaattat atatggaatt ttgggaaaac tacactagaa atatttaata cacaaggcag
    36481 gaataaccca tgaaaaaaag aaatataaat attttgctca tttccttaag tatcctcata
    36541 ttaataacag caggtgcttt gaatctgggg cagtatatta aacctaaaaa tccagataat
    36601 aaaaatcttg attctaaaaa ccctgacttc aaaaaacctg attctaaaag tcctgacatt
    36661 aaaaatcctg actttaaaag tcctgattct aaaaacccat attctaaatt ggaataccaa
    36721 ggtgaaagca gccctgtaac ctacagattc ccaggtgagt ttgaaaatca gcaggcaata
    36781 tggatgcaat ggccatcaca gatatataac gaaagcagtc atcctgtaaa tcctgttatg
    36841 attaatatta ttaaagcatt tgatccatat atacgggtaa atgttgcatc acaggatacc
    36901 gatgaaattg ttagtattga aaatatgctt aagcaaagcg gttatacagg cagcaatgtt
    36961 catttttatg ttgtaaagca cctttctata tgggcaagag atgtgggacc aatttttgta
    37021 aaagacagca ataataaatt ccatgttgta gattttgtct tcaacaatta tagcagaggt
    37081 ggaagccagt attatataga catagaaaat caggtgcata aacaaatagc aaatttatat
    37141 ggttatccac tcatcagtac aaacttgatc tctgaaggag gagctattga atcaaatggc
    37201 aaaggcacat taatgctaac agagtctgta gcccttaagc gcaactcaaa actcaccaag
    37261 caggagattg aaaacgaata taaaagggtt ctgggggtga gtaaaataat ttggctgaaa
    37321 aagggacttg cagaagatga tagtataaca acaggacata ttaacgaaat agcccgattt
    37381 gcaaaccccg atacaattct ccttgctcag gttttgccag cagacagata tactaactct
    37441 gattcaaacg aatcttattt gagacttgaa gaaaattatg acatactgca aaatgctact
    37501 gatcaagatg gaaagtcatt caagattata agagtcccca tgcctcctac aatttatcag
    37561 aagcctgttg aaacaggtaa aatacccgtg cgtagttatc taaattatgc cgtaacaaat
    37621 aatgctgtac ttatgcagac ttattggaga ccaggccgtt ctgataccct taaagctgct
    37681 gaagatgatg ttaaggctat acttgaggga gtatttcctg gacgtgatat agttggaatt
    37741 gattcagaag ccctaaacct gtggggtgga ggtattcatt gtgtaaccca gcacatgcct
    37801 gaatgcatat cccagtgatg aactggtgac cagaccagtt cattatcatt tttcaccggc
    37861 gaaagctaaa acagatatac tgtcctgttg tttaattcat cataaataat atgtgcctca
    37921 acatcaaaaa catacctgaa caaatctttt gtaagcacct tttctggttt tcctgaatca
    37981 tagattttcc catctttaag tgcatatatt ttgtttgagt atttagcagc ctgatttata
    38041 tcatggagca ccataacaac tgtcaggttc aagctttcat ttaagtcttt tattaggtct
    38101 aaaattcccg tctgatatga cacatcaaga aatgttgtgg gctcatccag cagcaatata
    38161 tttgttttct gtgcaagagc catagcaata tatactcttt gtttttcgcc cccggacagt
    38221 ttttttaaca aacttcctct aaaatcttga agccccgttt tctctatagc ccatgatact
    38281 atttccttgt cttttactgt aaatttctgt ccaaaactaa ggtgcgggaa tcttccaaac
    38341 tttactacat cttcaactgt gaagtcatca ggtgcctctc ttacttggga tagtatggaa
    38401 atttgtttag caacttcttt cgtatccatt ttgtgtatgt ctttaccatt caaaaggact
    38461 ttgccgtcgc aaggcttaat tattcttgaa aaagccttta atatcgttga tttgcccgaa
    38521 ccatttggtc caattatagc agatattttg cctttttcaa tctccatatt aatattattt
    38581 ataaccagtt ttctgccata accaagtgat aaatttttcg cacataatat cattttttac
    38641 caccccttct gcttctcccc attgctaaaa gatacagaaa aaatggggca ccaagggtag
    38701 acataataat tccgacgggt atttcaacag gactaatgat aactcttcct attgtatcac
    38761 aaatcataac agtaaaggcc ccaagaagaa ttgaagcagg aagtaaatat ttatgatcgg
    38821 ctcctcctgt taaaagcctt gctatatgcg gtacaataag ccctacaaaa cttaatagcc
    38881 ctgcaacgct tactgcactt gctgcaagaa gagctgataa aataatgaat ataaatttca
    38941 ctttttcaac acgaagtcca aggcttatgg ctgtttcatc acctaataag agtatgttta
    39001 gttttttggc taacagtaat gaaaagattg agcctaaaaa tgcataaggg agtatcatct
    39061 taaaatgatg ccatgttttt gcagataatc ctccaaccat aaatccataa acacctatag
    39121 ctctctcagg gtaaaatatc atcaatgcat ttattcctcc gcttaaaata gaggaaactg
    39181 caacgcctga tagtataagt cgtaaaggct gcaatccccc atcccatgcc aatagatata
    39241 caactgctgc agtgcccaga gcacctaaaa atgcagcaat tggtgctatg aagtaaaggt
    39301 ttggaaataa tataaaaatt ataaatgctg ccagcccggc tcctgatgat atgccgagta
    39361 ttccgggaga agcaagagga ttgcccataa ccccctgcaa tataacacct gaaagtgaaa
    39421 ggcatattcc aacaagagct cctgttagag ctctcgggag ccttacattc catattacct
    39481 ggtgatttgt cgtatctttc tctacgaaaa tagctctgaa tatttcctgg ggggataact
    39541 tgacagcccc gttttccaaa ctaattatca aacctgctaa acatagaaac accattagaa
    39601 caagaataac tatgttttta atattcctgt tatctttcaa tatattaaag aaattagact
    39661 tcatgattta tccaaccttg tttattgttt aaaataaaat actctagcgg aatactatat
    39721 taatactatt atgccagttt ggaaagcgat gtcttccgag ataattttta atagcgcttt
    39781 ccattggcaa cataacaaag taatattaag taatctaaca aataatctaa caaaaaatct
    39841 atcagcaatc tatctaaaaa cttcagggta cagcgttctt gccagttttt cataggcttc
    39901 aggatatctt gtattaggtt tataggtata aagctccttc tcaaggtata tcaacctgca
    39961 atttctgaca gcatcaagag aattccatgc gttatttttt cccaattcag tatctatcat
    40021 gttttttgtt tcttgtacgg ttccgcccat gctttcaaca aatataaagt ccgggttttc
    40081 ttctattatc ttctcaaggc tgaatatttg ggtttctgca tctagtactc caaatcctcc
    40141 ggctagtcca ctggctatat tcattgtccc taaatcttca agcattgagc ctacagagga
    40201 attcggcagt tttactgtaa ttcctcttga tgtagcaaat agcaaaagga tcttaatcgt
    40261 ctttctctca ttactgttat tctctagttc attatttact gtaccgttaa cctctgcccc
    40321 atcaccatta ataatcctat tactttcagc tactttaaaa acaatttcct ttaccctgtt
    40381 tacaacctca accccgtttt taatataaag atcgtttctt cctgttaact cagtaaaaat
    40441 cttcaatact ttcagatact ccataacatt ctgatacttt attgcaatat atgatatttt
    40501 gttttgcttc agcatatcaa taagattatt ttgtccgttc attgtgggac ttaatataac
    40561 aaggtcaggc tctaaagcaa ttatcttttc tatgcttgct gctcctgttt ttccaatgac
    40621 ttctgcatcc tttgaaggtt ctggaactgt tcttttactt tccactctgc ctataacctt
    40681 cccccctgcc aaatcccaca attccaggta tgaatcgtaa agacagacaa cacgctcagg
    40741 tctttttcta atggtcactc tatcatcaat agcatcaata aaagatacag tatcatcact
    40801 tatatctatg tcatcaagta tatctacact gtcaaatata tctttgccat catttatatc
    40861 tgtgtcattc ttttttgaaa tatggccatc atttagtata tcactagtac ctgattgaac
    40921 ataagtggtt tttgctttat ctatagtctt atgtccgcac ccattaagga atatggtaag
    40981 aactaaaaca aaagctgatg atgaaatttt tctctgcata tttgtttttt ccattgttgc
    41041 ttcattcctc ttgcatctgc ttttctcata ttgggatgca tttaatttaa gtgatgaaaa
    41101 tattggtaag ggctatatgt gaagctgctg gtaaatattt ctttactatt aaaaatgcac
    41161 ccctcatatt gttacacttt attgcatttt attgtcttca gccaaacaaa tccttttgta
    41221 ctttgtccca gcctatcctc tctatcataa gagcaagtct ctctccatcc ttggcatttt
    41281 ctttgaagta gtcaattatt tttcctgtta cttcttcaat ctcatctact ttgaatattc
    41341 tgtcaacacg tcttcctatt tgccactgtc ttccaaacct tcctccaata aaaagtgcaa
    41401 cacctgcagt ttcaactgtt atagcaccaa aggggcatgt tttggaacaa acaaggcaat
    41461 tcaagcattt gtccctgttt atttctgttt tattttccac atattcaatt gcttttgttt
    41521 tgcaggctcc tacacagagc ttgcaatttt tgcatttttc tctgtctatt ttaacataag
    41581 cctgccccat aaaacctata tcatttaaag aagcctttgc acaattattt gggcacccta
    41641 ccgcaccaat tttaagtttt gcaggcagtt ccatggcaaa atacttttca tgcaatttca
    41701 tgcaaatccc ctgtgtatca ataatgccat gagtacatac agtacctttg cagggaacaa
    41761 ttggtctgac tctttttccc gtacctccat gcctcatacc tgcatctttc agttcctgct
    41821 ttacattatc tatgtcctct tcctttatcc acggaatttc aacgcaaagt cttgttgtaa
    41881 aacccatata acctctgccg tatttttcag ctatctctga aagcttctcc atattccggg
    41941 aatccatatt ccctacgttt gttacaactc ttactgagaa ataaccctgt tgctgctgcg
    42001 caaggaagcc ctctgatttg agtttggcta tttgctgctt atccattttc atccccctga
    42061 aaacctttaa ttattcgtaa taatatacat atatgaagat aatcaaacta tttctcgatt
    42121 ataacataga atgctaataa tgtgtaatgt aatttttaat cgaattctaa atccatgtat
    42181 gaaatattct tgtaaaatct agaaacttca cttaatttag ttttttaccc ctttatcgaa
    42241 tacaaataat tgaataaaaa aatatgatgt gataaaattt tagcaacaat tgtacattat
    42301 gattaagggg gtttttttat ggcatatgtt ccagatgcaa acagatatac cgaaatgaaa
    42361 tataatcgat gcggtagaag cggccttatg cttcctgcta tttctttggg actctggcat
    42421 aattttggcg gcgtggattc ctatgaaaat tcaagagctc tgatacataa ggcatttgat
    42481 cttgggataa ctcattttga tctggcaaat aactatgggc cgcctccagg ttctgcagaa
    42541 gagaactttg gaagaatact aaaatcagac ttcacaggtt accgtgatga aatgatcata
    42601 tcgaccaaag caggatatag aatgtggccc gggccctatg gtgattgggg ttccaggaag
    42661 tatattctat caagccttga ccaaagcctt aaacgtatgg gcttggatta tgtagatatt
    42721 ttctattctc acagaccgga tcctgaaaca cttctcgaag aaaccatgtc agcacttgat
    42781 caagctgtac gtcagggcaa agccttgtat gtaggaatat cttcatatag tgctgagcag
    42841 acaaaaaatg ctgcagatat tttgaagaac cttggaacac cttgtcttat tcaccagcca
    42901 tcttattcta tgctaaatcg ttggattgaa gacggattgc aggatgtact tgactctgag
    42961 ggaataggca gtatagcttt ttgcccattg gctcaggggc ttcttacaac cagatacctt
    43021 tcaggcatac ctcaagattc acgtgctgcc aagtcttggg gatttcttaa gaaagaacgt
    43081 attactgaag atatattgac aaaaattaag catcttaatg atattgctgc tttaagaggt
    43141 caaacacttg cacaactggc attagcatgg gtacttagag gaggcagagt tacttcagcc
    43201 cttattggtg ccagtaaggt cagccaaatt gaagaaaata taatggcttt aaataacctt
    43261 aatttcagta aagaagaatt agatagaatt gaccaaatat tacaaggtta acatattgta
    43321 ggggacattt ctgtactatg catctgatat tccttcagca taaagaaatc ttcttatata
    43381 tttttatatt ttgagaagtg tccctttata ttttcacatt atactaatat tactttgtta
    43441 tgttgccaat ggaaagcaca tttccccgcg caacatcttc ccgatgctat caaaaattat
    43501 cctggaagac atcgcatcca aattggcata gtagtactaa tccttcttct ttttgtttcg
    43561 gaaaagggac agtcctccaa aaccgctgat tatagctatg taaaagccaa aatcatataa
    43621 ccatcctgta tttacaggtt catatactct tatattcctg tcgagtaatc caattattaa
    43681 tgatatggga gcaacccagc catgccatat ccctgagaaa aagcccgccg gtttctgtga
    43741 agtatatttg ccatcccctg gaatgcagcc ggttaatgca aaaataacaa atacagtaac
    43801 tgcaattact ataacagttt ttctcttcat aaaatcaccc caaaacaatt aattaataat
    43861 tataaccgat atatattata tcacaaaaac catttatagg tatgtaggta tggagaactg
    43921 tccccatata tatttgtata tttggagaac tgtcccccac atatttatag ttcaagcctt
    43981 taaccaaaac ctggtaagca ggaaaacaac caaagcaaat attgcgccaa atattaacac
    44041 ccacggcagc aatattgaaa gcaatgccat gaataatgct acataatctt taaatgtcat
    44101 taattttaaa gcatcttcac tatcttcact acagccatcg ccgctatttc ccgacatgtt
    44161 agctttagtt tcatccatac tttttgaatt tattacatta ctataattac atttattctt
    44221 attaatatca ggtctacttc tttttttaaa tatcaaaaag tcaacacctc atcactattt
    44281 tgtcaaatga catgcagcca tgtgtccgcc tccaacatcc ttaagctgtg gattttcttt
    44341 tctgcatttt tcaaatgcat atgggcaccg agtatgaaac ttacagcctg aaggtggatt
    44401 cgcagggctt ggaatatccc ctgttaatat aatcctgttt ttcctcaatg tggggtcggg
    44461 tatgggcact gcagagagaa gagcctttgt gtaaggatgc acagggtttt gaaaaagctc
    44521 atttgtctca gctgtttcta ccatagaacc aagatacatg actccaattc tgtgggacac
    44581 atgcttaacc acacttaaat cgtgagatat aaatagataa gaaaagccaa attctgactg
    44641 aaggtcagat aaaaggttta ttatctgtga ctgaatggaa acatcaagtg cagatactgg
    44701 ctcgtcacat actataaatt ctggttccag ggcaagagca cgtgcaatac ctattctttg
    44761 tctctgacct ccagaaaatt catgagggta cctgtccaca tgatagggca gcattccaca
    44821 acgttccaat atttcaagga catgttcctt aacattcctt ttagtgtacc tcccatgttc
    44881 tataagggat tcaccaacaa tatcaccaac agtcatacga ggatttaaag aactgtacgg
    44941 gtcctgaaat acaatctgca ttttaggcct taattcagtt aattcctttt tatttaatgc
    45001 atgtatatct tttcctttga acaaaacctt tccgccatct ttttcatata agcggagtat
    45061 ggtacgcccg gcagtgcttt tcccacagcc agattcccca accagcccaa aggtttcacc
    45121 ttttttcagt gtaaagctta cattctcaac tgcgcggaca tatcccacag ttttctgcaa
    45181 tacgccgcct tttattggaa agtatttctt taagttttcc acctctaata gtaattcact
    45241 catatttaca cctccttgta cagacggcat gctactttat ggccgttctc agacttgatc
    45301 aatggaggaa aggttgtttt gcagatttcc atacagttct cacacctttc ggcaaaatag
    45361 cacatctccg gcagccctat aggatttgga acattacctg gaatagagta aagacgtccc
    45421 tgagtctgat ctatttgagg ttttgacttt aaaagaccta tagtgtaagg atgtttcggg
    45481 tttttaaata tctccaatac cggcccttcc tcaactacct ttccggcata cattacaatt
    45541 acatagtcgg ctacttcagc cactacaccc aggtcatggg tgataagcat tattgacata
    45601 ccaaattctt tcttgacttt caacagcaga tcaagtatct gagcctgtat ggttacatct
    45661 aatgcagttg taggctcgtc agcaatcaac agttcaggat tgcatgccag agccattgct
    45721 atcataattc tctgcctcat accacctgaa agctcatggg gataagattt gtaaatacct
    45781 tcggcacgcg gaatacctac aacttttatc atctcaatag acttttgtct agcttcactc
    45841 tttgagagct tctcgtgcag catgattttt tctgaaatct gatctcctac agtaaaaacc
    45901 gggttcaaag aggtcatagg ttcctgaaaa atcatagata ttttactccc atttatttga
    45961 tgcatttgtg cagtagtgag tttcagtaaa tcagtaccgt tataaagaat ttctccttca
    46021 acaattttac ctggtgactg aataagcctc gttagtgata aagccgtaac acttttacca
    46081 cagcctgatt caccaacaac gcaaactgtt tctccttttt ttatattgaa tgaaacacca
    46141 tttacagctt taacaatacc tgcatcagta aagaaatagg tatgaagatt tttaaattca
    46201 acaagattat tagacatact tacctcctcc ttaccgttct tatcgtttca tcttggggtc
    46261 aaaagcatca cgcaagccat cacccacaaa gttaattgcc agaacagtta caaatataca
    46321 tacacctgat ggaatccata accaccagcg ataagcaaaa tcgtaggaat cttttactgt
    46381 ctgtatcata tttccccatg atgggtatgg catggccacg ccaaggccca ggaatgacaa
    46441 ggcagattcg cttaagattg ctccacctat tccaagggtt gcagacacaa ttatctgagg
    46501 aataacatta ggaatcaggt gacgtgctat cttccttctg tcgcttaagc caagtgcttc
    46561 agcagccagc ataaattcct gttcgcgcaa tgaaagtatc tgtcccctta caagacgtgc
    46621 tagggatggc cacccaagga aacctagaat aagcataaca aagaatatcc ttttctgagg
    46681 tggtacatca aggtctgaca taactgtacc aagaataatg accatgggaa gatatggaag
    46741 acttaaaaac acatcaacaa ttctcataag ggcactatct atcttgcctc cataataccc
    46801 tgcaatacca ccaattgcag ttcctataac aatctctatc aatacagcta caaaacctac
    46861 tgtaagtgat atcctgcccc cgtacatcag tcttgtaaaa atatctcttc ctattgcatc
    46921 tgttccaaaa atatatttaa aactaggctt ctgtttggct attgtaactt tagtctcaag
    46981 tagagcaaag tttgaaaata ccgggccata gaagcataat attataaata tagctaatat
    47041 gcccatacca acaagtgcca gcttattcct tctaaattgt cttaaggcaa tagaccaggg
    47101 tgtctgaatc aaatcctgtc tgtttctttc aatattctca agaaccttta cagcaatagc
    47161 aacaattaga ggcaaaagtc ctgcgataac aaaaacatgg gctcctattc ctgtggtaac
    47221 ttcttcctgt gcaattttat aaatatatga tccatcactg gcctgctgtt tcaccagtaa
    47281 atttgcaaag aattctatta cactggttcc tgtatctgat agttgcacac cgtataaaac
    47341 atatcccagt gaagcagtaa atatggaaaa acttgaaata tttttgcttt ttttaaatat
    47401 gcccactgtg aacagccccg ctgttaatgt gtacattatt agagagagta aaagcggcaa
    47461 tgaattgctt attatgacaa gaacaggccc gctgcctgaa acaggtgtaa gctcaactct
    47521 ctctaaaata tgaaaaacca gatcaaaacc actaatagat ccatatttct ttatattggc
    47581 aaatggtaat gtgaatataa taacaattat tattgaaaaa atgattcccg aaaacaataa
    47641 ggtttttctt ttcattattg cacctcactt aagctttaca cgtgggtcta ccaccgcata
    47701 aagaatatct gataaaaggt taccaattaa tgttaggaca gcaacaaaca tggagaagcc
    47761 catcaacagg ggataatccc ttttgtttac tgcatccaga gcaatcttcc ctattcctgg
    47821 tatgccaaaa atgctttcag taattattgc gcctgaaaac agtccgggca atgacagtcc
    47881 tattattgtt acaattggta ttagagcatt tcttagagca tgtttataaa taacagtctt
    47941 ctcacttaat cccttagatc gagcagtcct gatatagtct tgtttaataa cttccaaaac
    48001 cgcagttctt gtaaatctca ttaaagaacc aacaccacta aatgcaagaa ccaggcacgg
    48061 cattataaga tgggatataa catcccatag gtacctgata ccagtgtagt tagcaccagg
    48121 tgtagtcata tttgccagtg gaagtatctt taaatctaca gcaaatattt tctttaaaag
    48181 aagacccatg aaaaaagatg gtattgaaat tcccatcaat gcaaaaacag taaaaatcat
    48241 atccgttttt gaatactgct ttgtagcaga aataattcca atgggtatag ctattaaaat
    48301 ctccaatata aaagcaaata atgccagaat gaatgaattc cacacaaatg tattaattac
    48361 ctcagataca ggttttatat aaataaatga gtctccaaaa tcgcccactg ctgcaccttt
    48421 taaccatttc cagtacttca caggtatggg gtcgtttatg ccataaagat ccttaaggtg
    48481 ctcaatttgt tctgcagaca tttttggatt tgcctttgcc tcaataaaat ttcccggcac
    48541 taaatttatt attaaaaata ttaccaggga aatacctata ataataggaa tcatttgtag
    48601 tattcttcta attaaatact gccacatatt tcctcctccc cattatatgt caactgcccg
    48661 aacaaagttc gggcagtaac atacaaacaa catctttatg cgtttcatgc tgtgtatttt
    48721 tctatcttaa tgtcaatatt taatataaat gatttgcaat ttgcaaagta ttgcttactg
    48781 agccgggctt accagttcac atttataaag gttgtacaaa tattctctgt aagaagagag
    48841 ctcaggccct ttgactcttg catttgcaac ccacatgtca cttctctggt atacccagga
    48901 gcatggcaaa tcatcattta agactttata aagatccttg tagatagctt tacgttttgc
    48961 aacatcagtt tcattgatac catcgtctat gagtttatct gcctgaggat tcttgtactg
    49021 atatctgttt tgagctccct gtgaatggta aatattcttt gcatccgggt ctggagtcaa
    49081 gccccatgcc ataaaccaca tttcaacctg tttcttgtta accttatcaa caagtgttgg
    49141 ccaatcaagt gtttcaataa ctatatctat accaagtttt gcatagtcgt ctttcataac
    49201 cgggaggaga atatcggtta cagggtgccc tggcattgct gtaaatttaa ctgtgaacac
    49261 ttcgccatcc ttttctctct taccattggc attcaacttc caacctgctt cgtcaagaag
    49321 cttccctgct ttatcaaggt cgaaatcgta agcgttaatt ccttcatcgt tataagccca
    49381 tgatactttg gactgaggta tattgattac actgccataa tcaccgtaaa tctgcgcaac
    49441 aacatctttt ctgttcatag catagtagag ggcttgcttt actttcttat cctgatattt
    49501 cttaacagtg ttgtttatac ctacataccc ataaccattg gttgggaatc tgtatacatt
    49561 aataaattct gtgtttttag cttcttgtat attttcagca gatacatcac ccatatcaat
    49621 gtcggtttct ccaagcatta ctctttgtaa ttcttctcct gaaggtgtta cagagaagac
    49681 tacattttta atctttggag cagctttgaa gtaattttcg ttggcaacga gttccaaaga
    49741 cgcaccttct ttgtactcaa cgtatttata ctgtcctgtt cccattggat tgcctatatt
    49801 ggccttaact ttttcaagtt gtcctttttt aaagtcgact ccatatactt tctttgaaac
    49861 aattggcatg ttcaatgacc agattgcagg accacttggc ttatcacatg taacttcaat
    49921 tgttgtatca ttaataactt ttactccctc aattttttca gcaataccat ctttatatgt
    49981 ttcaaaacct ttgataaaag ctgatgatat atcaattggg ccatcataac ttgggtctga
    50041 gagtacatag tatgcaaatt ctacatcctc tgcctttaca ggagtgccat cccagaattt
    50101 taaatcttcc tttaatgtga aagtatactt gagaccatca tcagatactt tatagtcagc
    50161 agcacctgga attggtgaac catcgaaatc agcttctatc aaactgtcaa acattaatat
    50221 atttccatat atatcatatg cagtttcagc gtagaaagga ttaaatatac cattgaactc
    50281 agttacacct ataaccatgg tatcctttct atcaagtgcc acagctggtg acttggctgc
    50341 atttgatgct tttataaaat cgacgccaag cttagttaaa tcaattgatt cttcttttgg
    50401 accttcttct tttttttctg atcctacttg tgtttcagag ctaacttctt tatcagaagt
    50461 ctcatcatta tttttccctt ttcctaacca cactgctgta attgcaatta ttacaaccag
    50521 cgcaattgcc aatattggca ggtacttttt cataatacat tcctctcctt tacaatatta
    50581 aatagttata taattttata actttatgtt aataaagttg atttaattca ttttataaga
    50641 tttgatataa atttgcaata tcataattga aaatacttgc aaatacctgc aataataatg
    50701 aaatttttcc tgcatcaagt taatacttta atgtgttagc agtttatcac ttgcttgatt
    50761 taacagctca tctggctaaa atactgaagt ttctataaat ccatatactt ctcactacta
    50821 aaatcatgaa caaataaaat acatattaaa gcataataat gcaggattgg ctgcatttct
    50881 gcacatgagt gcatgggaaa tataccacaa aaatatataa tttatacgcc ttgttaccaa
    50941 tatttacatt ggaatatttc caaattgcat gttataatgc taccgataag ttattatgta
    51001 aacttattaa ctgcgtttgt ttgcccaaaa cactaaatag attcttaaat caacaacaaa
    51061 agggggtata tatgactagt tttaagaaac ttattatatc aggtacatta agcttaggta
    51121 tccttttttg cagtgcagga ataacacatg cccaggagat aagctataaa gtccagcagg
    51181 gtgatacctt ttggattgta agccagaaat atggagtacc tgttaatcgc ttaatgcagg
    51241 taaataaagc tactcagaat actatacttt atataggtca gaacatattg ataccaacag
    51301 attatgaaat aattcatact gttgtggcag gagacaccta ttggattata agtcaaaaat
    51361 atggggttga cctgtattca ttaatggcgg ctaacaaagc tacagaaaaa acaattcttt
    51421 atatcggaga caaggttgta attccgccta aacctcaagc accccaatat actctataca
    51481 caatacaagc tggagatacc tattggatta taagccggaa atataaattg gatgtaaaat
    51541 atttaatggc ctataatggt gcaaatgaaa atactatact atacattggg caaacaatta
    51601 aaatacctca accaggtgta tcaggaagta ctgcatcagg cgcaggtact gcatcaggcg
    51661 caggtactac atcaggtgca agtactgcat caggtgcaag tactgcatca ggtgcaagta
    51721 ctgcatcagg tactatatca ggcactacgc ctgtacaagt acctgtacag acacttaccg
    51781 gtacagaacc ttatattacc tatacacaat atacagtgca aaaaggagat attctgtggg
    51841 atattgctat taaattcgga attccacaaa ctgagttact gaaggcaaac aatctaactg
    51901 aattatctat tgcatatatt ggtgatatat tgaaaattcc tgtccatcat gtgccggtta
    51961 aggcgactcc aggagacaaa tacggtgaat accttgattg gtggactgaa gcacagtatg
    52021 ttgtgcctgt tggaagtgaa tttgaggtaa ttgattttta taccggaagg tcttttaaag
    52081 caaagcgtac tacaggagca aaccatgctg attgtgaagc tttaaccata agtgatgtaa
    52141 gtaaaatgaa ggaaatatgg ggaggtaacc ttagttgggt ttcacgtcct gttattatta
    52201 agtacaatgg tagaaaaatt gctgctagtg cagccagctt cccacatgca gggaatgata
    52261 atgctccagg cggaatatat actacatggc gaagcgggga ttatggagca ggttataacc
    52321 ttgattgggt taaagataat ggtatggatg gagtttttga tatacacttt ttaaacagta
    52381 caaggcattt ggatggaaaa atcgatttat ctcaccaggg taacataaag ccggcagcag
    52441 ggataaaata agttggtatg aaaagcaatc atgttgatga atactagaca ctttaattta
    52501 atggaatggg cacatttttt atctcactaa tctcgattaa tctcgactgg gtgcatttaa
    52561 tttaagtgaa gaaaatattg gtaaggagca taagtgaagc tgctgtagct gggcagggga
    52621 aactcttctc tcaagtatca ggcattcctt ttacgctaga gagtttcccc atatctgagc
    52681 gaaggaagag caaaattgtt gtttgagtaa aattgttact taatattctt tcagccaatt
    52741 ttatgagttc aattttgccc agagctgaat ttaaaggctc taagcagagt aacgtcagct
    52801 cctggtaaat atttcttcac ttttaaaata tgcacccatc tcgacttcat agattgcatt
    52861 taagttttta tcaaatccta ttagcctttt aattttataa tctgtttcct tactattcca
    52921 ggtaaacaaa aacatattat cgtaaagctg cggatattca aggatttttt tctctccatg
    52981 cagttccgct tccatcttta cagatataat tctattgtca tttacaacgc catataacat
    53041 ccaaagcata tagtcatccc tgtaggtgct accatgccag taataatcta ttgaacgcga
    53101 cacatcattt tccttgccat gtacctgatt tccaggatac cacataccta aacagccttt
    53161 cttaacagta ttacaagaaa accatttgtc atatttacat aaaaatagct tcccatattt
    53221 aaaattttgg gtttgtatta cttttgaagg cccgtagtta gctgttttct cagatttctt
    53281 atgcgcagaa agtgctgtta atgaagcact ggaaaacatg aagtgtataa aaagcaatgt
    53341 taacagtatt cctgtatttc ttataagttt atgtttttta ctcaaagctg tcacccccat
    53401 agcccccata aagaggtatc tcaaatcgca ttttgcggtt ataataatca taatcgagta
    53461 ttagtacagc agccgtttca ttaatcttct caattttaat aatatgcctt gttccgaaaa
    53521 tatttccgat acttgaagag cctccatagt actcatttcc taaatcatca tatacattaa
    53581 agtgaagcat ggaccaacca tgagaaaaag gctttgaata atctttagat aattataaag
    53641 tctttaaata atccttaaaa tgtctgaatc ttttgaataa acttattaaa tggtaactcg
    53701 taatattaac tgtacattta aataagtttc ttcaaaatta cagctatcct tccttttcgc
    53761 gttgtaccgc caatttcatc gataacaacc ctgccttttc cttttaccga tacggtatct
    53821 ccttctttaa cctgtgcaga aagactgtct tcaggttccc aattcaaata tactctgccg
    53881 gaactaaata atggtgcaac tttgctcctg gatattgtaa atccattact tacaatacag
    53941 tcaagtctta aagaaggtac agtacatttg atttcttttg ttctgggtac cgaaatttcc
    54001 aaattttcaa gttttcccag ccttgtatag acatttatat gacctatttt attaaggttg
    54061 tacaatatat agtctgcaac atctttcaat acaataatac tgcatgacct ttcgtccaca
    54121 atgatgtcac ctatcttctc ccttttaatt cccaatccca ttaaagcgcc cagataatcc
    54181 ctgtgcgaaa ggtatctttc tgttttaagt tttatttcta atagggttag atagtcagat
    54241 agggatggat ggttagacat ttgttcaggg ctgaatatta caattgttct ctccgcctcc
    54301 ttatatccgc catcaaattt atattctaca tcttttacac catgcagcgc actttcaatt
    54361 gtctttcgct gatatgggtc aagaaaatca gaacaggcag ctatccatga cttctcagcc
    54421 tgggctgcct tatcaattat cctggttaca agcattttgt cttcgatttt attaaatttc
    54481 ccgagtatgt ttttcttgtc tatcatgtta ttcacccata aattgaatag tatatcctga
    54541 tttatgggtg catataattt aagtggagaa aatattggta aggagcataa gtgaagctac
    54601 cgtagctagg caggagaaac tcttctctct gataattgat gttcctttta ttttgaagag
    54661 tttccccgta tctgagcata ggagaagcaa aattgttgtt tgagtaaaat tgtttcttaa
    54721 ttttttatca gacaattttg cgggatcaat tttgcccgga gctgaattta aaggctctag
    54781 gcagtgtaac gtcagcttct ggtaaatatt tcttcacttt taaaatatgc acccctgatt
    54841 tatctatcta tttatttata aagcaattgc agcagtggtg ataaaatgcc cattatgcct
    54901 ccaagaagtg caccaagcca ggttattgcc ttcaactcct tatttgctat ttcaattatt
    54961 atttcttcag caaaagctac atcatagctg tttatctgat cttcaacaac ctttggtata
    55021 tttaatagct caacaatatt tggcaatttc acctttacaa agttacctag gaaacttttt
    55081 ataaaattgg caatattctc aaacatattt tcatcaatat tatcaagtat attcgaaact
    55141 ggtttattca taaccatatc aattagccta ttaataatca aatatatatt atcatataat
    55201 gctgttgaat ttaaaatgct atcaagctta tttgatacaa attttaataa atttccctta
    55261 atatccggct ccgaggcttt tatttttccc tctaccatat caactacctt gccctgcttc
    55321 tctctatcag aaatataatc caatataata tccgaaatct gtaatattcc ttgttcctta
    55381 ttttcaggcg aaatacctga aactatattt gat
//
LOCUS       DAOSDD010000002        50919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_404_length_50919_cov_5.218878, whole genome shotgun sequence.
ACCESSION   DAOSDD010000002 DAOSDD010000000
VERSION     DAOSDD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 50919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50919
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_50919_cov_5.218878"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PK733_00195"
     CDS             complement(<1..1236)
                     /locus_tag="PK733_00195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOJ09000.1"
                     /translation="MNNITCRMLTSHFLFPACDLYNYVSSSQITAIISSKILNAPIFA
                     KLVEYCKELFYTILIVANCCFLMEGLLIMRFVYIDHLKEGIINARPIWGKNGELLLNA
                     GTKIQESYIVKLKELGYHGIYVDDALSHDIEVPVLIDDNLRLECVKTIKNAFEEIESI
                     KAIRSKTVDKLEKLIDDIIDIIQANKELLVNMFDLKVYDDYTFFHSVNVSVLSLAVGL
                     SLYSNRNELFKLGLAALLHDVGKVFIPRKILIKNSSLTNEEFKIIKSHPLKGYQLVKD
                     NNSFSHYFGIGILQHHERFDGSGYPFGLPGKKISLPSRIIAVADVYDALTSDRPYRKA
                     LFPSEAVEYIMGNGGILFEPHILKQFLKIITPYPVGTGVQLSNSKSGIVAKNYADCCM
                     RPIVKIIKHGDIMVDPYLID"
     gene            complement(1279..1620)
                     /locus_tag="PK733_00200"
     CDS             complement(1279..1620)
                     /locus_tag="PK733_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOJ09001.1"
                     /translation="MPRVNKTTATFTNSTEYLKNGLSVSPAIPSLGDKLTVQYSGLLA
                     QSGASHIYAHVGYGSNWDNLYDYPMNRTDKGFEVNIPVVKSGTINVCFKDCANNWDNN
                     SGSNYAFDVVQ"
     gene            1912..4371
                     /locus_tag="PK733_00205"
     CDS             1912..4371
                     /locus_tag="PK733_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HOJ09002.1"
                     /translation="MKAVIMAGGEGTRLRPLTCSRPKPMVPVANKPVMQYIIELLRKY
                     ELVDIAVTLQYMPETIKDYFRDGSEYGVNLSYYVENTPLGTAGSVKNAEEFLDDTFIV
                     ISGDALTDADLKSAVDFHNNKGSIATLLLKKVDVPLEYGVVVTDSESRVTRFLEKPSW
                     GEVFSDTVNTGIYILSPVVLTYFNRNEMFDFSRDLFPILLNEGMPMYGYIIDDYWCDI
                     GDPNAYIQSHIDIFEGKVKINIPGKEIMKNVWVGEGTAIDENVKIHSPCIIGKHTRVK
                     KNSLIGEYSIIGDSNIIGEGASIKRSIIWNNSLIDENVELRGNVVCNKVHFYNNSSAF
                     ENSVIGDDTVIKENAIIKPNIKIWPDKLIDEGTIVNSNLVWGAKYTRNIFGNRGIAGE
                     INNTITPEYASKLGAAYGAIFKEKGKIGISCDNNPSTKMIKSAFIAGLLTTGQEVYDL
                     GEMLLPITRSAIRFYRMDGGIHISSLRQNPARLFIDILDEKGCNINRGTERKIESIFI
                     REDFTRCEGDCIKEVTKIYDYSNYYLGNIINSVKAAKFNYKIVLNTSSDFTAEIMKNL
                     LNALGCEVELLNLKIVNSKTKRESMTSADVRFFTSHIKMGNYDLGVSVEDSSEKMMLI
                     DDKGRILTEDMYMALIAIMLFKSGGGGTVVVPLSTSHVIEKIAEEYNGKVIRTKTSTQ
                     DIMYKLLGNGEEEMFDQFTLHFDAIAGLVKILKFMSLNNYKLSTLVDMIPSFYMNRQE
                     VECPWNAKGKVIREIIQEQEGNTIETTEGVKIYKDGGWVLVLPDAELPICRVISESYS
                     AEFAKELSTAYADKIREISQK"
     gene            4642..5364
                     /locus_tag="PK733_00210"
     CDS             4642..5364
                     /locus_tag="PK733_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ09003.1"
                     /translation="MKKGSWFVLFLIYSILGFIISLQFRSNMSTKKEKSASVLNTERL
                     LAQVEQEKKTQTELKAEIEQNRKKIEEYLKSYIELNDDRKLKDLWTALYDAKLKAGFT
                     DVEGPGLVIKLDDAPSKKSDDPLLSIIHDADIQIILNELKKAGAQAISINGERIVATS
                     EQLCAGPTIRINKEVYSVPYRINVIGPPDDLYYSLMMSERVILMRKQGIRITVGRSNK
                     VLVEQYKKDPTKLISRLEVIGL"
     gene            5361..6053
                     /locus_tag="PK733_00215"
     CDS             5361..6053
                     /locus_tag="PK733_00215"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ09004.1"
                     /translation="MKIARNIAITVVCIILGVMVAWQYKSINYNQNAMSYQNKRAEGL
                     MEEVIRLQKNNADLRTQLQKLQNDVRLYESAKAGSDEASKTLLEQLKTARIFAGFYDV
                     KGKGIIITVESNIYTVLEDDILALVNELRAAGAQAISVNDERITAMTEIRDAPPYTMI
                     NKVPVTAPFTIKAIGDPEQLENSLKMISGVVENLQEFLKITIKKSDDISIPKVREDGS
                     VIKIDLLNPVSQ"
     gene            6181..7125
                     /gene="pdaA"
                     /locus_tag="PK733_00220"
     CDS             6181..7125
                     /gene="pdaA"
                     /locus_tag="PK733_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HOJ09005.1"
                     /translation="MTINLYEKTRKNHKPFRALGEVYNSDFKDLDLSIELSGRLSSDN
                     QYSDKKAYPENNKLNSSSILENNLKYSDYSNSVETSQNIVYSNKLLSWGILRKPNNET
                     PAADPGAPELLAKYNGIYLGDTSKKVIYMTFDEGYENGYTPKILDVLKENKVQAVFFI
                     TGPYLNKHQDLVRRMVEDGHIVGNHTVHHPSLPTLTDEEIEEEILGLERAFNEKFNRK
                     MQFLRPPKGEYSERTLAITNKLGYQNMFWSFAYDDWYRDKIRGPEYARNIVMNNLHNG
                     AILLLHAVSKDNADALDMIIKGAREKGYEFGDFDELKE"
     gene            7145..7483
                     /gene="yqfC"
                     /locus_tag="PK733_00225"
     CDS             7145..7483
                     /gene="yqfC"
                     /locus_tag="PK733_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HOJ09006.1"
                     /translation="MALSRKNIKDAGKNSKVKGEKRHADKNVKEKLTEVLELPKEVIL
                     NIPKMTIIGNKELVIENYKGIIEYGSARIRINTGTGLIKITGNGLNIKEITSEDVIIG
                     GDISSLEFQM"
     gene            7508..8707
                     /gene="yqfD"
                     /locus_tag="PK733_00230"
     CDS             7508..8707
                     /gene="yqfD"
                     /locus_tag="PK733_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOJ09007.1"
                     /translation="MLFLRLWNYIRGYVIILIEGYYLERFINICTHRQIFLWDVNRSS
                     DIAMTLKVSIQGFKLLRPVAQKTYCAIKIVDKKGLPFILSRYKKRKAFALGALLFFFL
                     INVLASFIWDIEITGNMEIGRMEIAEALFSMGIKPGTLKYKVDVDIVANELMLKMEKL
                     AWVGFEIRGTRALLYLKERQKPPELVAKDIPCNIIAGKDGIIKSVIVKAGQELVTAGD
                     TVTKGQILVTGVIKGKNENEPQKLVHAIAEIEARTWYEANGTVKFKTVETVRTGKTTE
                     IYRLKLLGKETNIPFLKKMDFVNYDKIEFRKVLSLGKDLVLPFELLTEKYYENELVEK
                     SLSVDEAKEAAENKAIKKLMDIIPEKAEILNRIIDFKQNNEDAITANIIIECIEDIGI
                     TEEIGGE"
     gene            8708..9676
                     /locus_tag="PK733_00235"
     CDS             8708..9676
                     /locus_tag="PK733_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOJ09008.1"
                     /translation="MEGQIEKAVELDRLDSAINLFGKCDENIKIIEEAFGIRVVSRDE
                     GIKIIGTCENVEKADKIVKKLINIAEKGETITRQNVNYLVQLAEEGLLEINSDYLDDC
                     VCFTVRGKQIKAKTYGQKNYVNAIKNNVICFGIGPAGTGKTYLAVAMAVTAFRNKEVN
                     RIILTRPAVEAGEKLGFLPGDLQNKVDPYLRPLYDALYDILGSETHHKYMEKGMIEIA
                     PLAYMRGRTLDDSFIILDEAQNTTPEQMKMFLTRIGFGSKVIVTGDITQIDLPGEKKS
                     GLKEVIKILKNIKGIEFIFLTEKDVVRHELVQRIIKAYERYDTIKS"
     gene            9750..12014
                     /locus_tag="PK733_00240"
     CDS             9750..12014
                     /locus_tag="PK733_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ09009.1"
                     /translation="MKGLRIFRLLKTYIVSTTFQKILIGILTLIISFIIVENGAVPKR
                     YKLYAGMVSPYDIPAPRDIENVLLTEQKAFEAANNVPAAIIRKVNIPIEVLNCVDDFF
                     MDLESARKKANGIIQEKGLTKKSKNYEEEWEKVQRQSVEELKEAVKELNLVVPLSDDQ
                     LSYLVGKISDDELVVFKKTIRELISEVMAQEVTNENLAFRINASQNSIQSKELNQEIK
                     NLGGHLIKAFIKPNSEIDIELTETKRKEAYNNFRENNKIIVKKNSRIVSVEEVITEDK
                     MQMLKELNLIETGKIDYVFAIGIMVILLLLALLLLVYMNFNCKHVLSNRNYIIVLCIV
                     IVMILFLARVLYVYSPLLIPFSIAAMLIAILIDFKLAIIANFILTLAISIMTKGDIAF
                     LYMSLVSGTLATFLVSRVNQRSSLTAAGLITSCINAIIIACFGILSKNQLKTIALDSA
                     IVFANGFVSVIITIGMLPLWETAFNLVTPLKLLELANPNQALLKKLLIEAPGTYHHSL
                     MVGNLAEVAIEALGGNPLLARVGAYYHDVGKLKRPDFFGENQISGNPHDKMNPSLSTL
                     VIISHTEDGVKIAEKYKLPQAIKEIIVQHHGTTMVAYFFHKAKKEDKTGTVKEEAFRY
                     PGPKPTSKEAAVVMLADSVEAAVRSMTEKTEGKIEGLVRKIIKDKLDDGQLDLCDLTL
                     KDLDTIANSFMRVFSGFFHARQKYPEIIKAGKEFLEEKVFVLPDTIVVNNKINDKTNI
                     DHEINMDDEISGRN"
     gene            12019..12510
                     /gene="ybeY"
                     /locus_tag="PK733_00245"
     CDS             12019..12510
                     /gene="ybeY"
                     /locus_tag="PK733_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ09010.1"
                     /translation="MIIFIENLQSKVGVSQEIEDIIRKAIELGLQKEGFDVPAEVSVI
                     LLDDEGIREINREQRNIDSPTDVLSFPLVDMENGKINSDLGDFDLDEEKILLGDIAVS
                     LEMAKKQAEEYGHSFERELAFLITHGVFHLLGYDHEDEESQKRMFSKQEAVLQDMGLV
                     RKG"
     gene            12534..12920
                     /locus_tag="PK733_00250"
     CDS             12534..12920
                     /locus_tag="PK733_00250"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOJ09011.1"
                     /translation="MEYEKLVKLAAEKRGKAYAPYSRFRVGAALITNTGNIYTGINIE
                     NVSYGASNCAERTAIFQAVSNGEDRIKIIAISSDSEDFIFPCGICRQVLVEFGCKNTE
                     IVCSKKNGEFVVYTLDQLMPHAFDKF"
     gene            13010..13903
                     /gene="era"
                     /locus_tag="PK733_00255"
     CDS             13010..13903
                     /gene="era"
                     /locus_tag="PK733_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOJ09012.1"
                     /translation="MDFKSGFIAVVGRPNVGKSTLINKITGEKISIISDKPQTTRNVI
                     KAIVTHEDYQMIFLDTPGMIKPKNKLGDYMIRVALESLEEVDAILFLIEATGERPGRG
                     DLQVMEQLKELKTPVFLVINKIDLISKEKLLSLIRVYKEYMDFEGVIPISALSGDGVD
                     ILLDEIKKVLPSGPQYFPEDMITDQPEKVIAAEIIREKLLLLLKEEVPHGIGVEILSF
                     TEKKDKNMVEIQANIYCEKDSHKGIVIGKNGAMLKQIGTMAREEIEKFLGLKIYLQLW
                     VKVKKDWRNSEQVLNILGYKR"
     gene            14081..14230
                     /locus_tag="PK733_00260"
     CDS             14081..14230
                     /locus_tag="PK733_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HOJ09013.1"
                     /translation="MLKEFVWNLFQKTGNVRSYVFYREIVERESALFEETKTRDEVAI
                     TGVHQ"
     gene            14292..15047
                     /gene="recO"
                     /locus_tag="PK733_00265"
     CDS             14292..15047
                     /gene="recO"
                     /locus_tag="PK733_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ09014.1"
                     /translation="MGYIKTQGLVIKEVNTGEADKIITLLSRDHGKIQVFAKGARRPR
                     SQFTACTRFLCFSEFTLFKGRDMHTINSCEVIEAFSEAGKDLIKLTYASHIIDIIFDT
                     VQEEQSSSGTLKLLLYTLYYLFKTDKSPELITRIFELRYLCILGYSPHVTGCVSCVKR
                     DLTGYFFSFNKCGFLCGNSECICKDASAIKILPGTARALHHIVYSKMNKLFGFDLAPE
                     VLKELKYITARYLRERLDKEYKGLNFLKSIDIT"
     gene            15157..15239
                     /locus_tag="PK733_00270"
     tRNA            15157..15239
                     /locus_tag="PK733_00270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15191..15193,aa:Leu,seq:gag)
     gene            15468..16391
                     /locus_tag="PK733_00275"
     CDS             15468..16391
                     /locus_tag="PK733_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09015.1"
                     /translation="MAGFFGFFDYTKPGPGIPEDAPPKAPIIVFFEIYFRKFWNLVKI
                     NLMFNLFNLPALLAVLFGTMYLIQGQITDDPAMDLMTRFIIGSVVICIPLITVGPAQA
                     GFTYILRNYSREEHAFLWWDFKDTAISNFKQSLIISVIDFFAFILFGIVVNFYFKYTA
                     NNNMIMTLMVFARTIIILAFLIYLMMHMYIYPMLVTFKLNLRQLYRNALIFSLAKFFP
                     NLGILILCTALVLLSFYFNTLIGIILFIFLTVSTIGLIINFYIYPKLKRYMIDKVADE
                     DTEVDEDEDYEDDVDDVEKSDNTEEDNNRIE"
     gene            complement(16649..17437)
                     /gene="dapB"
                     /locus_tag="PK733_00280"
     CDS             complement(16649..17437)
                     /gene="dapB"
                     /locus_tag="PK733_00280"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOJ09016.1"
                     /translation="MIKILLSGCNGKMGQVITRLAQQNTDLEIVAGYDINDSIKNQYP
                     VFTNIGNCNIHVDAIIDFSNPAALDNLLEYAVSKKIPLVMATTGLSDLHMKKLKEASG
                     FIPVFQSANMSLGVNLLIDLVKKASKLLEGYFDIEIIEKHHNQKLDAPSGTALAIADA
                     INTVIGQKQEYVYDRHARRKKRSKTEIGIHAVRGGTIVGEHSVIFAGNDEIIELKHTA
                     MSKEIFGTGAIKAALFIYNKQPGLYSMHDMIEQDNSSLTIKTSK"
     gene            complement(17465..18343)
                     /gene="dapA"
                     /locus_tag="PK733_00285"
     CDS             complement(17465..18343)
                     /gene="dapA"
                     /locus_tag="PK733_00285"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOJ09017.1"
                     /translation="MRKPIFTGAGVAIITPFTKDGINFEKLEELIEFQINQGIDSIVS
                     CGTTGEASTMPDEEHLSAIKFTVEKVNGRIPVIAGTGSNDTRHAIELSKAAEDAGADG
                     ILSVTPYYNKTTQKGLYEHFKAIASSIKIPIILYNVPSRTNLNINPDTIKALSEIHNI
                     YGIKECNLSQVGDIINLCGDDFTIYSGEDGQVVPLLALGGKGVISVMANIIPKDTHDM
                     VYKYLEGDIESSRKIQLKTLNLIKALFIEVSPIPIKAAMNLMGMNVGKCRMPLVDISE
                     KNLEILKSELTKYGLM"
     gene            18582..20285
                     /locus_tag="PK733_00290"
     CDS             18582..20285
                     /locus_tag="PK733_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOJ09018.1"
                     /translation="MQKTKKKIISIISKLMVTCLVVILILIQVQVKAESSNNNVFDEV
                     RVGMFFKNSSSPNVSNEISYFNISSPKGVEAGYLKDGEFFKIFDTGDGSVLTVRKDSY
                     YIIDGASIQECKPGANNLPEESKIGPFHIQVGGSYDNYETVKTEVDKYIQKGINVYPA
                     YTDSWYIWTGFYVDQNSAEQNIKNSLIPKIGEEKYSIVQPSKNNIVVTMKSNETRLIF
                     GNSEGYFQVRPRMENNPYIFNINGKNYRGIIETRRLEESDLTVINILPIEHYLYGVVP
                     SEIEWSSHPEALKAQAVAARTYIYNNMGKYSKLGFDVCSSVNSQVYGGFSVEKETTNK
                     AVDETQGKKLYYNSVLAQVFYFSSSGGRTEAAKNVWSEDLPYLQSVEDKYEQEGSKNY
                     TWEKTISAAKAGDIMVSLGNDIGEVLRITIDNTSEAGRAIKMTVAGTKGEKVFLNSRC
                     RDLLSLPSQWFTISTDSDAAVWNSSANEKISVQVGGKKVVSVNGSGTIAVNGNISVLG
                     DNGLKRIIPAVPSTYTFSGRGYGHAVGMSQQGAKGMANAGFTYEDILLHYFYGTNIKY
                     E"
     gene            20336..21361
                     /gene="queA"
                     /locus_tag="PK733_00295"
     CDS             20336..21361
                     /gene="queA"
                     /locus_tag="PK733_00295"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ09019.1"
                     /translation="MKVKDFYYELPKELIAQEPLKERSSSRLMVLEKSTGGITHEIFN
                     NIAGYLKQGDCLVINDTRVIPARLLGEKEDTGGKIEFVLLKKERDDIWEVILKPGRRA
                     RPGSRFVFGDGILKAEIIDILDEGNRLVKFEFEGIFEQVLDKVGIVPLPPYITKKLSD
                     SERYQTVYSKYRGSAAAPTAGLHFTNELIDSIKLMGVEVANITLHIGLGTFRPVKVDN
                     VEEHHMHSEYYKIDQETCNKINAAKKSGGRIIAVGTTSCRVLETASGQDGLLKSGEGY
                     TDIFIYPGYKFKMIGGLITNFHLPESTLIMLVSAFAGYDNVMEAYKTAVNERYRFFSF
                     GDAMFII"
     gene            21420..22556
                     /gene="tgt"
                     /locus_tag="PK733_00300"
     CDS             21420..22556
                     /gene="tgt"
                     /locus_tag="PK733_00300"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ09020.1"
                     /translation="MSAVKYELIKKCKQTGARLGVVHTPHGSFETPVFMPVGTQATVK
                     TMSPHELKEAGAKIILSNTYHLYMRPGHDIVKEAGGLHKFMSWDSSILTDSGGFQVFS
                     LSNLRDIHEDGVYFKSHIDGSRHFISPEKSIEIQNALGSDIIMAFDECIPYPSEYKYV
                     YDSVERTSRWAKRCKIAHQNTEKQSLFGIIQGGIFSDLRQKSAWEIMDIGFPGYALGG
                     LSVGEPAEEMYEMLECTLPLLPEDKPRYLMGVGSPDYLIEGAIRGIDMFDCVLPTRIG
                     RNGTVLTSRGRVIVRDAAYARDFSPLDPDCDCYTCRNFTRAYIRHLLKTNEVLGMRLT
                     TYHNIKFLIDLMKKIRQAIIEDNLGDFRKNFFNSFGYTKSTTNI"
     gene            22591..22896
                     /gene="yajC"
                     /locus_tag="PK733_00305"
     CDS             22591..22896
                     /gene="yajC"
                     /locus_tag="PK733_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOJ09021.1"
                     /translation="MPEEGAAGLAGLGGVGFILYIVFIIGIMYLLVFLPQKKRDKKAK
                     EMISSLKVGDKVVTIGGLAGKIINIKDDEVTVETGVEKTKMTLKNWAIKEVDKPIEA"
     gene            23191..23649
                     /locus_tag="PK733_00310"
     CDS             23191..23649
                     /locus_tag="PK733_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOJ09022.1"
                     /translation="MLKKSLQKKSSSSDEQLKLTILLKGVILAYIITIPFFILFSLIL
                     SYTNFPENYISTAVTSVTIISVLISSTSVSRNIKNKGLFSGAFIGFFYMFILYIINGI
                     VFKNYSITRHLITMTFICILTGSVGGIMGVNIGTGKRSRIKSRPKRGRVR"
     gene            23756..23896
                     /gene="scfA"
                     /locus_tag="PK733_00315"
     CDS             23756..23896
                     /gene="scfA"
                     /locus_tag="PK733_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOJ09023.1"
                     /translation="MKHIKTISASVLKRSAKNSGCGECQTSCQSACKTSCTVGNQKCE
                     KN"
     gene            23984..25348
                     /gene="scfB"
                     /locus_tag="PK733_00320"
     CDS             23984..25348
                     /gene="scfB"
                     /locus_tag="PK733_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOJ09024.1"
                     /translation="MKEGFIHKFSLNGTNTVVDVNSGSVHIFDPVAYEVLDYYAEDFK
                     EEEIIAKLGDKYGKEQVHEAFKEITQLKQEGMLFSEDEYHFSVAKNNDIQFKAMCLHV
                     SHDCNMRCRYCFASKGDFGSGRKLMDVETGKKAIDFLIKNSGKRRNLEVDFFGGEPLM
                     NFDVMKDIVSYAREKEQEYNKNFRFTVTTNALLLDKDKMDYINENFVNVVLSLDGRKE
                     INDKMRVRVDNSGTYSHIIQKIKDMAESRNQENYYVRGTYTRENIDFSNDVLHLADEG
                     FKQVSVEPVVAAAGSGYDIREEDLPEIKAEYEKLALEYVNRNNSGSGFNFFHFMIDLE
                     HGPCAEKRYKGCGSGFEYIAVTPEGDIYPCHQFVGIDEFKMGNVYDGNIDDKIVCTFK
                     NCNITSKDECSRCWAKFYCGGGCAANAFNFNKDINKPYKVGCELEKKRVECALWIKTQ
                     IHSL"
     gene            25529..26815
                     /gene="secD"
                     /locus_tag="PK733_00325"
     CDS             25529..26815
                     /gene="secD"
                     /locus_tag="PK733_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOJ09025.1"
                     /translation="MRRSSWVGFVLVVVIIALLAFLALDGGNAIGINGISKTRTGIDI
                     KGGIYTTLYPDLPEGQKPTEEDLNSARMIIEKRLESKGIYDKNVTVEKEHGRIIVEIP
                     FAPGEKDFNPQKAVDEIGKTALLTFQEVDETKVDEKGDFLPTGRIVIEGKDVKTAGVA
                     TMPDTGEIVVTLELNKEGAEKFAEATKRLLGKRIAIFMDDSYITAPVVNTVIPDGSAQ
                     ITGQRTAKEAGELADTIRAGALPFRLVAKELNSVSPILGEGALKVTIKAGIVAFILVA
                     AFMIAYYRLAGLIADIALSGLVVTTLLLISVTGNTLTLPGIAGIILSVGMGVDANVVI
                     FERIKEELKNRKTIGAAVDDGFRRAFTAILDSNVTTIITAAVLYWLGSGAIRGFALTL
                     GIGVAISFLTGVTATRVMLKSICSAKFAKNPKLYGA"
     gene            26829..27791
                     /gene="secF"
                     /locus_tag="PK733_00330"
     CDS             26829..27791
                     /gene="secF"
                     /locus_tag="PK733_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOJ09026.1"
                     /translation="MIDLMGKKKIFFAISIFVILAGVVGLIVNGGLNYDIQFQGGTII
                     QVEMSDDNFDANNIGSVVSDMLNKKVSSQKLKTYNPKNEAESINILMLKVPSDHTLSD
                     TEINNIVDLLRKDYNVNPEAEIQMQSVQPSIAAEIKQKGITAILVSSALIIVYIWWRF
                     SNISGLPAAITAVIALMHDALVMLSAYAIFKIPLNESFIAAVLTILGYSLNDTIIIYD
                     RIRENSGKLRKLPVNELVNTSITQTMGRTINTSVTTLIAIVIVYIFASANNIQSIKEF
                     SFPLIIGIISGTYSSIFIASPLWMMWKERQSKAKIVAKQAKART"
     gene            complement(27935..28213)
                     /locus_tag="PK733_00335"
     CDS             complement(27935..28213)
                     /locus_tag="PK733_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09027.1"
                     /translation="MNKTLGIDKTIKKIYNELCKLNKHTISFIKLGANISIFLFASGT
                     LLSVLNRFVFNHDPYKEFIATSVINSSFTILAEVVIGCLLVDYAFSNK"
     gene            28666..30471
                     /gene="dnaG"
                     /locus_tag="PK733_00340"
     CDS             28666..30471
                     /gene="dnaG"
                     /locus_tag="PK733_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOJ09028.1"
                     /translation="MYNYFNDEFIEEIRVNNDIVDVVSEYVHLEKKGRYYFGLCPFHN
                     EKTPSFSVTPALQIFNCFGCNKGGNVIHFIMNIENLDFAEAVKFLADRAGIRLPDEKN
                     VNDEEYKLKKDILDINRTVAHYFYDNLMSSRGVKGQEYLRNRGIHPATLRKFGLGYSL
                     PEGNNIFGLFVDKGFSYKAIISSGLVIKSKSGEYFDRFKNRLMFPIFDVRGNVIGFGG
                     RTLDGSLPKYINSPETLVYNKGKHLYALNFAKSIRKKNLIVVEGYMDVITLHQNGIIN
                     SVAPLGTALTENQGRLLKKYAEEVIISFDADTAGQAAAIRGLDLLAGIGCTVKVLSIP
                     DGKDPDDFVRKNGADEFRKLIDGSRNLVEYKIGCLKNRIDTSTTEGKLKFVKKAAEII
                     SRLDSRVDVEVYVKKFSEQYGISVEAFYSEVLRSVQPGKNIKEVKFYNEKKHNDVNAA
                     SGKKVGNIDLKANYSELLLFAILCIDNNAYKLIKDKIGVADFSAENKPIAEIVFNRLQ
                     SNYGIVSAELLNIMEAEKAGEFARILEQECNFDDSRKAAIDVVNKIFITKLENRKREI
                     LEMLKNKENMQNLQTSELLKELNELVVVIKKHSEY"
     gene            30489..31568
                     /gene="rpoD"
                     /locus_tag="PK733_00345"
     CDS             30489..31568
                     /gene="rpoD"
                     /locus_tag="PK733_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOJ09029.1"
                     /translation="MKVNNETRKTILKELIEKGKSKGMLTYKEIMDAFEEIELDPDQI
                     DKIYETVENMGIDIVGDLEEDVEDIQINEEDLDLSVPEGISIDDPVRMYLKEIGKVPL
                     LTADEEIELARRMEEGDNEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLI
                     KAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLL
                     QELGREPLPEEIAKEMNMPVEKVREVMKISQEPVSLETPIGEEEDSHLGDFIPDDDAP
                     APAESAAFTLLKEQLMDVLDTLTPREEKVLRLRFGLDDGRARTLEEVGKEFNVTRERI
                     RQIEAKALRKLRHPSRSKKLKDYLD"
     gene            31963..32038
                     /locus_tag="PK733_00350"
     tRNA            31963..32038
                     /locus_tag="PK733_00350"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31996..31998,aa:Asn,seq:gtt)
     gene            32042..32118
                     /locus_tag="PK733_00355"
     tRNA            32042..32118
                     /locus_tag="PK733_00355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32076..32078,aa:Ile,seq:cat)
     gene            32310..33011
                     /locus_tag="PK733_00360"
     CDS             32310..33011
                     /locus_tag="PK733_00360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ09030.1"
                     /translation="MKLKGRLRAIAEMVPKCNLICDIGTDHAYIPIYLVKSSICKRAI
                     ITDIKQGPINIAENNIRENHLEDCIETRLGDGLHPLDNNEIDVLIIAGMGGKLMAKIL
                     WEGISKAMNANLLILQAMNGLELVTQWLFEEGFDICDEELVEDDNRIYNIKAARWTGV
                     KKVYNNVDLHISKKLIEKNNPLLIEYIDRKLAVLDKIISGLQKTNKNSAEAETELYKM
                     EILKKDMTEIINNMR"
     gene            33017..33829
                     /locus_tag="PK733_00365"
     CDS             33017..33829
                     /locus_tag="PK733_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ09031.1"
                     /translation="MSATKCKGIVDFMENIAPVYLAEDWDNVGLIIGDYERDINHILV
                     SLDVTTQILDYAIEKSVDMIISHHPLIFKPIKRIVYDDWKSVLISRIIKNDICLYCAH
                     TNLDYAKNGVNWNLALALDLNDIKNYGETKSEGEGYSLAKVGLLKPPMKLSMFVSNIK
                     HILGIDSVKLISNNADKAFERDVNKVVVFSGSFDDSVLRYIGDDIDVIITGDVKYHTA
                     VEIAEKGVCVIDAGHFATENVIVPQLCSQLKDRFPDLSVTSKMVVNQPIKFY"
     gene            33896..34243
                     /gene="rnpB"
                     /locus_tag="PK733_00370"
     ncRNA           33896..34243
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK733_00370"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(34284..35348)
                     /locus_tag="PK733_00375"
     CDS             complement(34284..35348)
                     /locus_tag="PK733_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09032.1"
                     /translation="MEKIRMGVIGCGGMAKSHLLGLLELSDKMVVTAVCDIVPERVQE
                     AAKVIGAEIAVTDYREMVNNVDAVLISLPHDLHFETGMFFLKAGKHVLMEKPMCNTEE
                     ECIKLIETAEKYKKVLMTAYPVRYWPIILKMKELVDAKTYGEAFQMSIWTEQFTKYPE
                     GHWALSAKRLGGGQFFSHGCHYIDLLLWFLGKPVKGFHMGTNFGTPWMENEGTSNAVI
                     EFENGAMGYHFGTWGARGTRLGYSFHIHCTEGMLEYSKADGQGKLYLHSHIKGEKANL
                     DTTSRTQVLMVETDQSKKTQFETLHFLNCIQNDTRPFTDGPASLQGLRIIWRMYEAET
                     KNIIADLNGLGLDEDWRNVK"
     gene            35501..36382
                     /locus_tag="PK733_00380"
     CDS             35501..36382
                     /locus_tag="PK733_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09033.1"
                     /translation="MVRARYILQNSLTIDKLITFYYMELTSKFMTRGERHDFWEFVYV
                     DKGEIEILTDSKQYELNQGDIIFYKPNEFHAGRAKNNTNPNLIIISFECSAPCMEFFE
                     GKTFRLDEEERKILSRLIKEGVGAFDPPVDSPNMRLPRRRQNAPFGSEQLIKNHLEIL
                     LILMIRKGDKRQENERLQTITSENREEDLTAQIKEYMKSCICENLTPEQLCTAFAISR
                     TQLMTMFKAKTGLGVKEYFNKLKIEKAKEMIRSGQYNFSQIAGYLSYNSVHYFSKQFK
                     FITQMTPTEYARSVKSR"
     gene            36561..37370
                     /locus_tag="PK733_00385"
     CDS             36561..37370
                     /locus_tag="PK733_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOJ09034.1"
                     /translation="MAEENKVTFTKHYSENENTCEKRYKGTYGDVCDNDETAENETST
                     DIDRTIGAAKVSNPRGNIHCLTIIGQIEGHIILPPQNKTTKYEHIIPQLVAIEEDDEI
                     NGLLLILNTVGGDVEAGLAIAEMIASMSKPTVSLVLGGGHSIGVPMAVATNYSFIAPS
                     ATMTIHPLRLNGLIIGVPQTYEYFDRMQERVVQFVSKNSKISRDEFRSLMLKTGELAN
                     DVGTVLFGEEAVQNGLINELGGLSRALEKLYELIGMRRKQVVLPNQSTNIN"
     gene            37399..37674
                     /locus_tag="PK733_00390"
     CDS             37399..37674
                     /locus_tag="PK733_00390"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HOJ09035.1"
                     /translation="MILYSIIPLDVVFGNNNEKFGELFEIEYMGEKVQVYSSTEDEYV
                     INRVISTSPKAFLRAELQPGSFINANIADIRVKSKATNARGFSIYHK"
     gene            complement(37785..39977)
                     /locus_tag="PK733_00395"
     CDS             complement(37785..39977)
                     /locus_tag="PK733_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ09036.1"
                     /translation="MKPLKKIFKVPLNVLKKIPFLDSKSLYSIRTKLIAAFMALVIPM
                     VLLGAISFSTSSKSIKKLATSSTENTMIQAQNYLNLLFSNVESFSMQIYLDQNIQDLL
                     TNKDGRFSKTDFDMLQIRQDAEKSFSRTTFSNKFIKNVLLIGDENKILASGINNTYGY
                     NIDSIKDTELYKKAAAAEGKLLWAGNHSEIDNITGSVPLDFSLSAVRIVKSMTLSKPV
                     GFLMLDLNINIVDSLFDDMRFSEDDEFFIVSPDGYQYSSVNKEVSNEANVNKTDKSED
                     NQSKKDKTLVQINITEQPFYKQIIKSEEAKGSSIVSVSKKKYLMSYQKIGESGYILFN
                     LIPESTLLSSSRKIAQTTVLLVILSVLCALAIGVFMAMGIGNIINKINIVANQASTGN
                     LTVDTESDRKDELGFLAGNISSMLGNMRGLIKQVMGLTRTVSTAADTVSTTAQEVSSV
                     SNEISIAVQEIAQGASSQASDAEQSTIKMSELANEINNVFENAKAIEDFSLKTLDFTR
                     EGLSSIKDLTEKSSETISISNSILQDIDVLGKNSESIGEIVDVISKIASQTNLLALNA
                     AIEAARAGEMGRGFSVVATEVRKLAEQSMSASREISVLIKSIQKQTIQTVERAERAGK
                     VINSQNEAVVGTTAVFEKISSSMELLVEKIRQIIEYITKMEHNKEDTMNSIQSISAVS
                     EETAAYSQEVTASIEEQVSSIEELANYAKDLNDAASKLLEEINKFKIE"
     gene            40229..42706
                     /locus_tag="PK733_00400"
     CDS             40229..42706
                     /locus_tag="PK733_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOJ09037.1"
                     /translation="MQSQKKQKKKYIVKKKQAKSSIKQPKHSTNKSNNSVYNEITEIL
                     ILSLGLLIFLSYYIDDSMGIFGSLVKKILTGMAGIPSFLIPLMLIVFPILMIFKKDKA
                     TMRKKGIYAFILLSLVCAIFHSGTYKYFDYVNRSPVDCIVKFYNDGKGFIGGGVIGGV
                     ISLPLLLLFQTLGTIVILTTAAIIMLILITDISLHEITRNVISSILTGSRNLVRGIRE
                     GREMDVIEDSNLYEFGSHMNSANTNEIYTEGKDEGSGFTLNIKGGEEVYVTGDSNDEQ
                     QTKVVDIKQHRKAKFLTLNNEKALEVEVKQQQCGSEFKTEVQMIDKESEKSIPENDKK
                     VAGKNVREMFYKYPPISILNSNQENGKNLKNYKNTAIEGAKKLEETLKSFGVEAKVVN
                     VSIGPVITRYEIQPSSGVKVSRIVSLADDIALNLASSGVRIEAPIPGKAAIGIEVPNK
                     EVTPVLLREVIESQEFNKHPSKVAFSVGKDISGECVVADIAKMPHMLIAGATGSGKSV
                     CINSLIMSILYKSSPDEVKMLLIDPKVVELGVYNGIPHLLIPVVTDPKKAAGALNWAV
                     QEMINRYKLFADKGVRDIKGYNELVSNSDEQDRLPLIVIIIDELSDLMMAAPGDVEDA
                     ICRLAQMARAAGMHIVIATQRPSVDVITGLIKANIPSRISFAVSSQVDSRTILDIGGA
                     EKLLGKGDMLFYPAGRQKPLRVQGAFVSDKEVENTVEFIKSQCNHEYDNAIIDEIDNS
                     SAKEENLDSEYDELLPQAIEMVVEAGQASVSLVQRRFKVGYARAARIIDQMEARGIVG
                     GFEGSKPRQILITKQQWQEMKMSVEQG"
     gene            42832..44391
                     /gene="rny"
                     /locus_tag="PK733_00405"
     CDS             42832..44391
                     /gene="rny"
                     /locus_tag="PK733_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOJ09038.1"
                     /translation="MSAIFVYGLLLGLLIAIIASFIMFRLGINYRKKVAEAEIGSAEQ
                     EAKRILSEAQKAAESKKREILLEAKEEIHKSRMELDKEIKERRNEIQRFERRLVQKEE
                     TLDRKLESLEQKDELLNKKVKDVQAQEEKVTGVLENHLKELERISGLTVEDAKEYLLK
                     SVESEVKHEMAILVKELESKAKEEADRKAKEIISCAIQKCAADHVSEITVSVVPLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREVARISLEKLILDG
                     RIHPARIEEMVEKAKKEVENTIRQEGDNATFETGVHGLHPELIRLLGKLKFRTSYGQN
                     VLYHSIEVSRLAGIMASEIGVDVNLAKRAGLLHDIGKAIDHEVEGSHVMIGADLVKRY
                     KEGNEIINAILAHHGDVEPTSIISVLVQAADSISAARPGARRETLESYIKRIEKLEEI
                     ANSFTGVEKSYAIQAGREVRIIVKPDDVSDEEVYLKARDIVKKIESELEYPGQIKVNV
                     IRETRAIEYAK"
     gene            44551..45336
                     /locus_tag="PK733_00410"
     CDS             44551..45336
                     /locus_tag="PK733_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOJ09039.1"
                     /translation="MKILFVGDVVGNPGRKTVKMILPEMKRNLSIDYCIANCENAAGG
                     SGITYAVAQELFNSGVDVITLGNHTWSKKEIVNFIDTETRVVRPANYPQELPGKGATV
                     IGNDNKGSIGVLNLLGRVYLDSVDCPFRTAEKEIQYLKNFTKVIVVDMHAEATSEKTA
                     LAWYLDGKVSCVLGTHTHVQTADERILPCGTGYITDAGMTGPYEGILGVDREIIVNKF
                     LTYMPAKFELAKGSTQFNAVLLDIDEKNGRTYRIKRVLQVMNV"
     gene            45452..45715
                     /locus_tag="PK733_00415"
     CDS             45452..45715
                     /locus_tag="PK733_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOJ09040.1"
                     /translation="MDVLKVSAKSNPNSVAGALAGVIREKGSAEMQAIGAGAINQAIK
                     AIAIARGFVAPSGIELICIPAFADIVIDGEERTAIKLIIEPRK"
     gene            45850..46653
                     /gene="pgeF"
                     /locus_tag="PK733_00420"
     CDS             45850..46653
                     /gene="pgeF"
                     /locus_tag="PK733_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOJ09041.1"
                     /translation="MKKDELEYMQFSPLTKYNQILTHCFTTRRGGVSIGGCSTLNLGF
                     GRNDSRTNVIENFKRVCDTLNIELDDLVLSDQIHGTEIKVVDEKDRGKGIVLDSDIKG
                     YDGLVTNIEKVALVTFYADCVPIYFFDPVKKVIGLAHSGWRGTLYQIAANMISIMDKE
                     YNCNPEEVISVVGPSIGQCCFEIGIEVITEFVKKIIWSKDFCKNIGQGKAYIDLQGII
                     KQTLIHSGVRSDNIFISGICTKCNKDVFFSHRGDKGKTGSLAAIMQLRR"
     gene            46658..48388
                     /locus_tag="PK733_00425"
     CDS             46658..48388
                     /locus_tag="PK733_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ09042.1"
                     /translation="MSKARGIFLSMVIIVILLISMSSCQKEDYILKNEKQNSVLPSLK
                     EDGAVVDGGPVKGGTLKLFSTFPDTLDPITSKNPWLQNYFSLVFESMIKLNKNQKPIG
                     NLADKWYTSEDGNTWTFHIRDGVVWQDGYPLTAQDVEFTIKTILERNQNTIYRFNLDN
                     ILSFTAIDRNTIEIVLKKPNAFTAELMTFPIISKRFYSKIDNNDKEVFATKFPPGTGP
                     YSIQSYDENKCIKLTASRSWWKKKINENNEMPYIPDLEIKLYKNTREAFSAFQMGSVD
                     VINVDNNSIGIYSGKSDVITKKYANGEFDFLAFNLSNNTLSDKYVRQAIASSLNKSEI
                     VGAVLPGCAVEAGMPLIPNTWLYEMDEWVKSFSNNSQKTKQSDNVRELLIENGWEEYD
                     GKLYKRIYGALRPLKFNIIVNEDNNCRVKVAEKIAEILNNTGMDVSVHKEKWNQVLKL
                     VNSKMYDAAILGCTVPPYPDVSYLYSAPYLTYQGYAASSAATAATNVSGFENEEVSKI
                     IEKVFSSNNPDTIKGLYSNLKNVINDEVPYIGLYFYYDAVFYNKNVRGNLDPYLWDEY
                     NNITEWYISE"
     gene            48529..48990
                     /gene="lspA"
                     /locus_tag="PK733_00430"
     CDS             48529..48990
                     /gene="lspA"
                     /locus_tag="PK733_00430"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOJ09043.1"
                     /translation="MLWIAIIILITAADQVSKYIVIKSVETGQLIPVINNFFYITYHE
                     NAGAAWGILQNKRYIFIFLTIVISIVMIYVLFKTKDKMLKLALSFVLGGAMGNLIDRI
                     IKGSVADFLDFYFGKYNFPTFNVADSFVVIGTIILAVYLLFYYEETEIIKL"
     gene            49065..49997
                     /locus_tag="PK733_00435"
     CDS             49065..49997
                     /locus_tag="PK733_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ09044.1"
                     /translation="MKILKLVSDTNNERLDAWLATKLKEYSRSYLQKLISEGFIFIGN
                     NNVKPNYKLKIGEEILIKIPEAEKLAVEPENIALDIIYEDDCILVINKPKGMVVHPAA
                     GNYSGTLVNAVLNYCGDKLSDINGIIRPGIVHRLDKDTSGILVIAKNNEAHKLLSQKL
                     KARDVKRVYAALVKGIIKENSGRIQLPIGRHPVDRKKMAVNPKNGREAITNFKVLERF
                     GDSTYVEISLETGRTHQIRVHMAYIGHPVIGDEVYGGKQKEYGQNNMKGQVLHAKLIG
                     FNHPATGEYVEYEASLPEYFKSLLAEKSSNNVKI"
     gene            50231..50776
                     /gene="pyrR"
                     /locus_tag="PK733_00440"
     CDS             50231..50776
                     /gene="pyrR"
                     /locus_tag="PK733_00440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOJ09045.1"
                     /translation="MLEKAQIMDEVEITRAISRIAHEIIERNKGVDNIVLIGIQRRGV
                     PLAQRIARKITEVEGKSVPVGILDITFYRDDLSMLAEHPVINATELNFIVTGKKIILV
                     DDVLYTGRTVRAAIEAIMDLGRPEMIQLAALIDRGHRELPIRADYIGKNVPTSRNEVV
                     HVKLVEIDNVNKVVITQRGND"
BASE COUNT        17972 a         7350 c        10288 g        15309 t
ORIGIN      
        1 atcgattaaa tatggatcaa ccataatatc accatgtttt ataattttaa caattggtct
       61 catgcaacaa tcagcatagt ttttagcaac tattcctgac ttgctattac ttaattgaac
      121 accggtgccc accggatagg gtgtaattat tttcaaaaac tgctttaaaa tatggggttc
      181 gaagagtata ccgccattcc ccataatata ttctacggct tcggaaggaa agagtgcttt
      241 cctatatggc ctatcggatg tgagggcatc ataaacatca gccactgcaa taattcggct
      301 gggcagtgat atttttttcc ccggcaatcc gaaagggtaa cctgatccgt caaatcgctc
      361 atggtgctga agtattccaa taccgaaata atgagaaaat gaattattat cctttacaag
      421 ttggtatcct ttaagggggt gtgacttaat aattttaaac tcttcgtttg tcaagctgct
      481 attctttatt agaattttcc tggggatgaa taccttaccc acatcatgca aaagagctgc
      541 taatcctaac ttaaaaagtt catttctatt actgtataac gataaaccaa cggcaagtga
      601 taaaactgaa acatttactg aatgaaaaaa agtataatca tcataaacct taagatcaaa
      661 catatttaca agaagttcct tgtttgcttg aataatatct ataatatcat caatgagttt
      721 ctcaagttta tcaacagttt tgcttcttat ggcctttata ctttcgatct cctcgaatgc
      781 atttttaata gttttaacgc attctaaacg caaattatca tctatcaata caggaacttc
      841 tatatcatgt gaaagtgcat catcaacata tataccgtga taccccaatt cctttaactt
      901 aactatgtaa gattcctgga ttttagtacc ggcatttaag agaagctcgc catttttccc
      961 ccaaattggt cttgcgttaa ttattccttc ttttagatga tctatataaa caaacctcat
     1021 aatcaataac ccttccataa gaaaacaaca attagcaact attaatatag tataaaacaa
     1081 ttccttacaa tattcaacaa gttttgcaaa aatgggtgca tttaaaattt ttgaagaaat
     1141 gattgccgta atttgactgc tggatacata attatataga tcgcatgcag gaaataagaa
     1201 gtgggaagtg agcattctac aggttatatt attcatagat caaacctctt aaccctagct
     1261 tcccacttta tccaattatt attgtactac atcgaaagca tagttgctgc ctgagttatt
     1321 atcccaattg tttgcacaat ctttaaaaca tacatttatt gttcctgatt ttactacagg
     1381 gatatttacc tcaaaacctt tgtcagttct attcatggga taatcataaa ggttatccca
     1441 attgctgccg taacctacat gggcataaat gtgtgatgca ccactttgtg caagtaagcc
     1501 gctatattga acagtaagct tatcgcccaa agaaggtatt gcggggctaa cgcttaatcc
     1561 atttttcaaa tactctgtac tattagtgaa agttgctgta gttttgttta ctcttggcat
     1621 taaaaattac ctcctttcta ttattcttgt atttcctgat ttattattta cctatagaat
     1681 tttaaaaata cagcacaacc tttaaggatt attaggaata aatgccataa tgaacctgca
     1741 taacaattta ttactattag cagtaatatt atacaaataa attactttta tctgtgcttt
     1801 tttaataatt ctgttggttt atgaattatc tatttgtgaa taggttttgc tttttttgca
     1861 gataatatta caaccttata acttttttaa taattggaaa ggatgataaa tatgaaggca
     1921 gttattatgg ctggcggaga aggtacaaga ttaagacctt taacctgcag cagaccaaaa
     1981 cctatggtac ctgtagcaaa taaacctgta atgcagtata ttattgagtt attaagaaag
     2041 tatgaacttg tagatatcgc tgtaacattg caatatatgc cggaaacaat taaggattac
     2101 tttagagatg gtagtgaata tggcgttaat ttgtcatatt acgtcgaaaa tactcccctg
     2161 ggtactgcag gaagtgtaaa gaatgcagaa gaattccttg atgatacgtt tattgtaatt
     2221 agtggagacg cacttactga tgcagattta aagagtgctg tagattttca taataataaa
     2281 ggttcaatag ccacactact gcttaaaaaa gtagatgtac cgcttgaata tggggtagtt
     2341 gtaacggata gtgaaagcag ggtaacaaga tttctggaaa aaccaagctg gggtgaagtg
     2401 ttcagcgaca ctgtcaatac tggaatatat atcctttctc ctgttgtgct aacctatttt
     2461 aatagaaacg aaatgtttga ttttagcaga gatttatttc ctatattgtt aaatgaagga
     2521 atgccaatgt atggctacat aattgatgac tattggtgtg atattggcga tcctaatgct
     2581 tacatccaat cccatattga tatttttgaa ggaaaagtaa aaataaatat tcccgggaaa
     2641 gaaataatga aaaatgtgtg ggtaggtgaa ggtacagcta tcgatgaaaa tgtaaaaatt
     2701 cattcacctt gtattatagg taaacacaca agagtaaaga aaaatagctt aataggtgaa
     2761 tattcaataa tcggtgattc taatattata ggagaagggg caagcataaa aagaagcatt
     2821 atatggaata actcactaat agatgaaaat gttgaactaa gaggaaatgt agtatgcaat
     2881 aaagttcact tctataataa ttcatcagca tttgaaaatt ctgtaatagg tgatgacacc
     2941 gtaataaaag aaaatgctat cattaaacca aacataaaaa tctggccgga caagcttatc
     3001 gatgaaggga ctattgtaaa ttccaatctt gtatggggtg caaaatacac aaggaatatt
     3061 ttcgggaaca gaggtatagc aggtgaaata aataatacta taacacctga atatgcatca
     3121 aaacttggcg cagcatacgg ggctattttt aaggaaaagg gaaaaatagg cataagttgt
     3181 gacaataatc cttctactaa aatgattaaa tcagcattta tcgcaggttt gcttactact
     3241 ggtcaggaag tatatgatct tggagaaatg cttttgccta taacaaggtc tgcaattaga
     3301 ttttacagaa tggatggtgg aattcacata agttctctca gacaaaatcc tgcaaggttg
     3361 tttattgata ttttagatga aaaaggatgc aatattaaca gaggtactga aagaaaaatt
     3421 gaaagtattt ttattagaga agattttacc agatgcgaag gagactgcat taaggaagtt
     3481 acaaaaatat acgattacag taattattat cttggaaaca ttattaatag tgtaaaggct
     3541 gcaaaattca actataaaat agttctgaat acaagttctg attttactgc agaaatcatg
     3601 aaaaatcttt tgaatgcact cggctgtgaa gtagagctgc ttaacctgaa aattgtaaac
     3661 agcaaaacaa aaagagaaag tatgacatca gcagatgtta gattttttac gagtcatata
     3721 aaaatgggta attatgatct gggtgtatca gttgaggatt cctcggaaaa aatgatgctt
     3781 atagacgata aagggcggat attaactgag gacatgtaca tggctcttat agcaataatg
     3841 cttttcaaat caggcggagg aggtacggta gttgtacctt tatctacaag ccatgttatt
     3901 gaaaaaattg ccgaggaata taatgggaag gttataagaa ctaaaacatc cacacaggat
     3961 ataatgtata aattattagg aaatggtgaa gaggaaatgt ttgatcaatt tactcttcat
     4021 tttgatgcaa tagccggact tgttaaaatt ttgaagttta tgtcattaaa taattataag
     4081 ctttcaacgc tggtggatat gattccatct ttttatatga atagacagga agttgaatgt
     4141 ccctggaatg caaaaggaaa agttatacgg gaaataatac aggaacaaga aggaaataca
     4201 attgaaacta ccgaaggagt aaaaatatat aaagatggag ggtgggttct tgttttacca
     4261 gatgctgagc ttcctatatg cagggttata agcgaaagct attcggcaga atttgccaaa
     4321 gagctaagta cagcatatgc tgataaaata agggaaataa gccaaaagta atctttttaa
     4381 taactagaac ccttttcaga aggtgcattt aatttatctg aagaaaatat tgtcaaggga
     4441 cataagtgga ggctctaaac accgtaacta tagcccctgg ttaatatttc ttcagatttt
     4501 ataaatgcac cccttttcag attttcatct tgcaaaataa gtatattaaa agtaaaataa
     4561 aagtatggtt gctgcaaaac aggagcaatt agttttacaa tgttgtgtac gatgttgtat
     4621 ataatgacag gagttttagt gatgaaaaaa ggcagctggt ttgtgttatt tttaatatat
     4681 tctattttag gctttattat atcgcttcaa tttagaagta atatgagtac aaaaaaagaa
     4741 aaatcagcct ctgttttaaa taccgaaaga cttctggcac aggttgagca ggagaaaaaa
     4801 actcaaactg agcttaaggc tgaaatagaa caaaatagaa agaaaattga agaatattta
     4861 aaaagttata ttgagttaaa tgatgataga aagttaaaag acttgtggac ggctctatat
     4921 gatgcgaaat tgaaagcagg ttttacagat gtagagggac ccgggcttgt aataaaactt
     4981 gatgatgctc cttcaaaaaa atctgatgat ccattgcttt cgattataca tgatgctgat
     5041 atacaaataa ttcttaatga gcttaaaaaa gcaggagcac aggcaatttc cattaatgga
     5101 gaaaggatag ttgcaacaag cgagcagctt tgtgcgggcc cgacaattag aataaataag
     5161 gaggtttact cggtccctta tagaattaac gtgattggtc ccccggatga cctatactat
     5221 tcattaatga tgtctgagag ggtaatattg atgcgtaaac agggaataag aattacagtt
     5281 ggcagatcaa acaaggtttt agtagagcag tacaagaaag accctactaa gcttatttcc
     5341 aggcttgagg tgattggatt atgaaaatag caaggaatat tgcaattaca gttgtgtgta
     5401 ttattttggg agtaatggtt gcatggcagt acaaaagtat aaattacaat caaaacgcca
     5461 tgagttacca gaataaaaga gctgaaggat taatggaaga ggttataagg ctgcagaaaa
     5521 acaatgcaga tttaagaacc caattacaaa aacttcaaaa tgatgttagg ttgtatgaaa
     5581 gtgcaaaagc aggaagtgat gaagcatcga aaacgttgct cgaacaatta aaaaccgcaa
     5641 gaatctttgc aggcttttat gatgtaaaag gcaaaggaat aataataaca gttgaaagta
     5701 atatctatac tgtattggaa gatgatattc ttgctttggt aaatgaactg cgggcagcag
     5761 gagctcaggc aatatctgtt aacgatgaac ggattacagc tatgacagaa ataagagatg
     5821 cacctccgta tacaatgatt aacaaggtac ctgtaacagc accgtttacg ataaaagcca
     5881 ttggagatcc tgagcaattg gagaactcgc tgaaaatgat tagtggtgtt gtagaaaacc
     5941 tgcaggaatt tttaaaaatt acgattaaaa aatcagatga tatttccata ccaaaagtga
     6001 gggaggacgg ctcagtaata aaaatcgact tattaaaccc ggtttcacaa tgataaatag
     6061 tattatttct ttactccctg aaatataaat attatagata tttatttttc agggagttga
     6121 tatactttgt ataaaagaat atttgttcta ttcttaatat ctatagtttt agctggattt
     6181 attacaatta atctatacga aaaaacaaga aaaaatcata agccttttag agctcttgga
     6241 gaagtttata attctgattt taaagatctt gatttaagca tagagttaag tggaaggctt
     6301 tcaagcgata atcaatattc tgacaaaaaa gcatatccag aaaataataa gcttaatagc
     6361 agttcaatat tagagaataa tctaaaatat tcagattatt caaattctgt ggaaacctct
     6421 caaaatattg tctacagtaa taaactttta tcatggggta ttttaagaaa accaaataat
     6481 gaaactccag ctgcagatcc gggtgcacca gaactgcttg caaaatacaa tggaatttat
     6541 ttgggagata cgtcaaaaaa ggtaatctat atgactttcg atgaaggata tgagaatgga
     6601 tatacaccta aaatccttga tgttctaaag gaaaataagg tacaggccgt atttttcata
     6661 actgggccct atttgaataa acaccaggat ctggtgagac gtatggtgga agatggtcat
     6721 atagttggaa atcacactgt acatcatcca agtcttccta ctttgactga tgaggaaata
     6781 gaagaggaga tattgggact ggaaagggca tttaatgaaa aattcaatag aaaaatgcaa
     6841 ttcttgcgcc ccccaaaagg ggaatacagt gaaagaactc ttgcaataac aaataaactg
     6901 ggttatcaga atatgttttg gagctttgca tatgatgatt ggtatagaga taaaatcaga
     6961 ggccctgaat acgcacgtaa catagtgatg aataacctgc ataatggtgc catactattg
     7021 ctgcatgctg tttcaaaaga caatgcagat gcccttgata tgataatcaa aggtgcaaga
     7081 gaaaagggtt atgagtttgg agactttgat gagctaaaag agtagttaaa ggagagttaa
     7141 caaaatggct ttgtccagaa agaatatcaa ggatgcaggt aaaaacagta aagtaaaagg
     7201 agaaaaaagg catgccgata aaaatgtaaa ggaaaaactt actgaagttc ttgaactacc
     7261 aaaggaagtt atacttaaca tacctaaaat gactataata ggtaataagg aattggtaat
     7321 tgaaaactat aaaggaataa ttgagtatgg aagtgctaga atacggataa atacaggcac
     7381 aggattgata aaaatcaccg gtaatggttt gaatataaag gagatcactt cagaggatgt
     7441 aattataggg ggagatataa gttcattgga attccagatg taattgttgc agctatggag
     7501 gttaaaaatg ttatttttaa ggttatggaa ttatattaga ggatatgtta tcatattaat
     7561 tgaagggtat taccttgaaa ggtttattaa tatctgtaca cacaggcaga tatttttatg
     7621 ggatgttaac cgctccagtg atatagcaat gacattaaaa gtaagcatac aggggtttaa
     7681 gttgctaaga ccggtagcac aaaaaactta ttgcgcaata aaaattgtag acaaaaaagg
     7741 tctgcccttt attctaagca gatataagaa aagaaaggct tttgcgctgg gtgcattgtt
     7801 attttttttc cttataaatg tactggcctc atttatatgg gatatagaaa taacaggcaa
     7861 catggaaata gggagaatgg aaattgctga agcactattt agtatgggga taaagcccgg
     7921 aactttaaaa tataaggttg atgtagatat agttgccaac gaactgatgc ttaaaatgga
     7981 aaagctggct tgggttggct ttgaaataag gggtaccaga gctttgctat atttgaagga
     8041 aaggcagaag ccgcctgaac tggtagctaa agatatacca tgtaatatta ttgctggaaa
     8101 agatggtata atcaagtcag taatagtaaa agcaggccag gaactggtaa cagcaggtga
     8161 tactgtaaca aaaggacaga ttcttgtgac tggagtaatc aaaggtaaaa acgaaaatga
     8221 accacaaaaa cttgtccatg caattgcaga aattgaagca agaacttggt atgaggccaa
     8281 cggtacggta aaatttaaaa ctgtagaaac cgtacgcaca ggtaaaacta cagaaattta
     8341 caggctgaag ttacttggca aagaaacgaa cataccattt ttaaagaaaa tggattttgt
     8401 caattatgat aagatagaat ttagaaaggt attatcgttg ggaaaggatc ttgtactgcc
     8461 ttttgagtta ttaacagaga aatactatga gaatgaattg gtagaaaaga gtttgagcgt
     8521 tgatgaagct aaagaagcag ctgaaaataa ggcaattaaa aaacttatgg acattattcc
     8581 ggagaaagcg gaaattttaa acaggataat agattttaaa cagaacaatg aagacgcaat
     8641 aactgcaaat ataataatcg aatgtattga agatatagga attacagaag agattggggg
     8701 agaatgattg gaaggacaaa ttgaaaaagc tgttgagctt gacagactgg actcagcaat
     8761 aaatttattt ggcaaatgtg atgaaaacat taagattatc gaggaagctt ttggaataag
     8821 ggtagtttcc agggatgaag gaattaaaat tatcgggact tgtgaaaatg tcgagaaggc
     8881 tgataaaata gtaaaaaagt taattaatat agctgaaaag ggagaaacaa ttacacggca
     8941 gaatgttaat taccttgtac agttggcaga agaaggtttg cttgaaatta acagtgatta
     9001 cctggatgac tgtgtatgct ttactgtcag gggaaaacaa ataaaggcta aaacttacgg
     9061 acaaaaaaat tatgtaaatg cgataaagaa caatgtaata tgttttggga taggacctgc
     9121 aggtacagga aaaacgtatc ttgctgttgc tatggctgta acggctttta gaaacaaaga
     9181 agtcaacagg ataattctta caagacctgc tgttgaagcc ggcgaaaaac tgggcttttt
     9241 accaggtgat ctgcagaata aagttgaccc ttatttgaga cctttatatg atgccctata
     9301 tgatatacta ggctccgaaa cccaccacaa atatatggaa aaaggaatga ttgaaattgc
     9361 acctcttgct tacatgaggg ggcggactct tgacgattct ttcataattc ttgacgaagc
     9421 tcagaatacc accccggaac agatgaaaat gtttttgaca agaataggat ttggatcaaa
     9481 agtaatagta acaggagata taacacagat tgatcttcca ggagaaaaga agtcaggtct
     9541 taaggaagta attaaaatat taaaaaatat taaaggtata gaatttatat tcttaactga
     9601 aaaagatgtt gtaaggcatg agctggtaca gagaattata aaagcttacg aaagatatga
     9661 tacaataaag agctgaaaag atgaaattta gttaggccaa aggcggtgaa tactaattgt
     9721 cgctatacaa aaatagagag aagatcgata tgaaagggtt aaggatattt agattattaa
     9781 aaacatatat tgtcagtaca acattccaga aaatattgat tggtattcta acccttatta
     9841 tttccttcat aattgtggaa aatggtgctg ttccaaaacg gtacaagctt tatgcaggaa
     9901 tggtatcccc atatgatata cccgctccgc gagatattga aaatgtttta ttgacagagc
     9961 aaaaagcctt tgaagctgcg aataatgttc cggctgcaat tataagaaag gtaaatattc
    10021 ctatagaagt gctgaattgt gtagatgatt tcttcatgga ccttgaaagt gcaaggaaaa
    10081 aagcaaatgg aataattcag gaaaaagggt tgacaaaaaa gtccaaaaac tatgaagagg
    10141 aatgggaaaa ggtgcagagg cagagtgtag aagaactgaa agaagctgtt aaggagctta
    10201 atcttgttgt acctctttct gatgaccagt taagttatct tgtgggcaaa attagtgatg
    10261 atgaattagt agtttttaag aaaaccatta gagaactgat tagcgaagta atggcccagg
    10321 aagtcaccaa tgaaaatctg gcgtttagaa ttaatgcttc acagaacagt attcagagca
    10381 aagaattaaa tcaggaaatt aaaaatcttg gtggacatct tataaaggca tttattaaac
    10441 ctaatagcga aattgatatt gaacttaccg agacaaaaag gaaagaggcc tataataatt
    10501 tcagggaaaa taataagata atagttaaga agaattccag aattgtaagc gttgaggaag
    10561 ttattaccga agataaaatg caaatgttaa aggaacttaa cctgatagaa acaggtaaaa
    10621 ttgactatgt ctttgctata ggcataatgg tcatattgct attgcttgca ttacttctcc
    10681 tggtatatat gaatttcaat tgtaaacatg ttctgtcgaa taggaattat attattgttc
    10741 tttgtatagt tattgttatg attttatttt tggcaagggt tttatatgtc tactcaccac
    10801 ttctcattcc tttttcgata gcagcaatgt taatagctat tctcatagat ttcaagctgg
    10861 ctataatagc aaatttcatt ttaaccctag ctatttcaat tatgacaaaa ggtgatattg
    10921 ccttccttta tatgtcattg gtaagcggaa cccttgcaac gtttttggta tcccgcgtaa
    10981 atcaaaggag cagtctcaca gcggcaggct taataacttc ctgcataaat gcaataatta
    11041 ttgcatgctt tggaatatta agcaaaaatc agcttaaaac gattgcacta gatagcgcta
    11101 tagtttttgc caatggattt gtttcagtaa taataactat tggcatgctg cctttatggg
    11161 aaactgcatt taacctggta actcctttaa agcttttgga gcttgccaat ccaaatcagg
    11221 ctcttttaaa aaaattatta atagaggcac cgggtacata tcatcatagt ttaatggtag
    11281 gcaacctggc tgaggttgct attgaggcat tgggagggaa tccacttctt gcaagggtgg
    11341 gtgcatatta tcatgatgta ggcaaattga aaagacctga tttttttgga gaaaatcaaa
    11401 tatcaggtaa tccacatgat aaaatgaatc caagtctgag tacgctggta ataatttcgc
    11461 atactgagga cggagtaaaa atagcagaaa aatataaact gccacaggca atcaaggaaa
    11521 tcattgttca gcatcatggt actacaatgg ttgcctactt ttttcacaaa gccaaaaaag
    11581 aggacaagac aggcacagta aaagaagagg ctttcagata tcccggaccc aaacctacga
    11641 gtaaggaagc agcagtagta atgcttgctg attcagtgga agcagctgta aggtctatga
    11701 cagaaaaaac agaaggaaaa atcgaggggc ttgttaggaa gatcattaaa gacaagcttg
    11761 atgatggtca attggattta tgcgacctta ctctcaagga tttggataca attgcaaata
    11821 gctttatgag agtttttagc ggcttttttc acgcaagaca aaaatatcct gaaataatta
    11881 aagcggggaa agaattttta gaagaaaaag tttttgtatt gcctgatacc attgttgtaa
    11941 ataataaaat aaatgataaa acaaacatag atcacgaaat aaacatggat gatgaaataa
    12001 gcggaaggaa ttaatagtat gattatattt attgaaaatt tacagagtaa agttggagtg
    12061 tcacaggaaa ttgaagatat aatacgaaaa gctatagaac ttggcttgca gaaagaaggt
    12121 tttgatgtac cagcagaagt gagcgtaata ttgcttgatg atgaaggcat aagggaaata
    12181 aacagagagc aaaggaatat tgattcgcct accgatgtgc tttcttttcc ccttgttgat
    12241 atggaaaatg gaaaaataaa ttcagattta ggagattttg atctggatga agaaaaaatc
    12301 ttgcttggag atattgcagt atctcttgaa atggctaaaa agcaagctga agaatatggg
    12361 cattcttttg aaagagaatt ggctttttta attactcatg gagtatttca ccttcttggc
    12421 tatgatcatg aagatgaaga aagtcagaag cgtatgttct ccaaacaaga agcagtcctg
    12481 caggatatgg gacttgtaag aaaaggttga tatatagtta agaggtgaag aggatggaat
    12541 atgaaaagct ggttaagctt gccgcagaaa aaaggggaaa agcatatgca ccttattcca
    12601 ggtttagggt aggagccgcc ttgataacta atacgggtaa tatttacaca ggtataaata
    12661 tagaaaatgt atcatatgga gcaagcaatt gtgcagaaag gactgcgatt tttcaagctg
    12721 tatctaatgg ggaagacaga attaaaataa tagcaatttc cagtgacagt gaggatttta
    12781 tatttccatg tggaatatgc agacaggtac ttgttgaatt tggttgtaaa aatacagaaa
    12841 ttgtgtgcag caaaaagaac ggagaatttg ttgtgtatac tttagaccag ctaatgcccc
    12901 atgcatttga taaattttaa gttatatata acgcaaaaat gatttaacta tattttataa
    12961 agtatcatct tttgcgcgtt atctttaggc tacaggaggt aataaattaa tggactttaa
    13021 atccggtttt atagcagtag taggaagacc gaatgttggt aaatcaactt taattaataa
    13081 aataacaggg gaaaaaatat ctataatatc cgataaaccc caaaccacaa gaaatgtaat
    13141 taaggcaata gttacacatg aagactacca gatgattttt ttagatacac caggtatgat
    13201 caagcccaaa aataagctgg gggattatat gataagagta gctttggagt ctcttgaaga
    13261 agtggatgct attctttttt taattgaagc aacaggggaa agaccaggca gaggtgattt
    13321 gcaggtaatg gaacaattaa aagaattaaa aactcctgtt tttctagtaa taaacaaaat
    13381 tgatttaata tcaaaagaaa aactattatc tttaataaga gtttacaaag aatatatgga
    13441 ttttgaaggt gttatcccaa tatcagcttt aagtggcgac ggtgtggata ttttgcttga
    13501 tgaaattaag aaagtattac cttccggccc tcaatacttt cctgaagata tgataactga
    13561 tcagcccgag aaggtaattg ctgcagaaat tatcagggaa aaacttcttc tgctcctaaa
    13621 agaggaagtg ccccatggta ttggagttga aattctttca ttcactgaaa aaaaagataa
    13681 aaatatggtt gaaatacagg caaatattta ttgtgaaaaa gactctcata aaggcattgt
    13741 tataggtaaa aacggagcaa tgttaaaaca gattggtaca atggcccgtg aagaaattga
    13801 aaaatttctt ggactcaaaa tatatctgca attatgggta aaggtaaaaa aagattggcg
    13861 taatagtgaa caggttttga atattcttgg ctataaaagg taatctaagg aagagctttg
    13921 gaatagttcc agtatacaaa atgtaatttt gagaaatata ataatgtaga tgttttattt
    13981 attaatttac accttgaata actttgaggc ttgtctcgaa ggattgaact aagggttttg
    14041 gtatattcaa caattgaaaa agaacagggg gaataatatt atgttgaaag aattcgtatg
    14101 gaatttgttt caaaagaccg gtaatgtaag aagctatgtt ttttatagag agattgttga
    14161 aagagaaagt gcattatttg aggaaacaaa aacaagggat gaagttgcaa ttaccggagt
    14221 acatcaataa taaaaattct aattatatta tttgaatgag gaaatgctaa taatcaggtt
    14281 aaaggttaaa aatgggatat ataaaaacac aggggttagt aattaaagag gtcaacaccg
    14341 gggaagcaga taaaataata actcttttat caagggacca tggtaaaatt caggtatttg
    14401 ctaaaggagc acgaaggcca agaagtcagt ttactgcgtg tacccggttt ctatgcttta
    14461 gtgagttcac actttttaaa ggacgtgata tgcacactat aaatagttgc gaggttattg
    14521 aagcattttc agaagctggg aaggatctga ttaagcttac ttatgcttct catataattg
    14581 atataatatt tgatactgtt caggaagagc aaagctcttc aggtacttta aaactacttt
    14641 tatatacact ttattacctg tttaaaactg ataagtctcc tgaactaatt acaaggatat
    14701 ttgaattgcg ctatttgtgt attcttggtt actctccaca tgttacaggt tgtgttagtt
    14761 gtgtgaaaag agatttaacg ggttattttt tcagctttaa caaatgcggt ttcctgtgtg
    14821 gtaatagtga atgtatctgt aaggatgcat cagcaatcaa aattttgcca ggcactgcca
    14881 gggcgctgca tcatatagtt tattcgaaaa tgaacaagct gtttgggttt gatttggcac
    14941 cggaggtact taaggaatta aaatacatta cagcaaggta cttaagagaa agattggata
    15001 aggaatataa aggtcttaat tttttgaaaa gcatagatat aacttgataa tgtttaagtc
    15061 tactaaatat aaataaaata taataaaaat aaaaaatcaa aaaagtattg acagagcata
    15121 tagatgctag tataatttac tttgcaggtt aaaaatgcgg tcgtggtgga attggcagac
    15181 acgtacgttt gaggggcgta tgcgcgagca tatgggttca agtcccatcg accgcaccaa
    15241 caaaaacagc tattaatatc aaagttaata gctgtttttt tgtgaatact aaagtaaaaa
    15301 ttttatttgt atacactttt tattaaatga gatataaaaa aactctgctt tatttattaa
    15361 aaacatattg aacatagtac aaactactgg taaaatggtt ataaatatat atgagaatgt
    15421 catgtagaat cagcgtgtac attctccagt taaaggggat atgcttaatg gcaggatttt
    15481 ttggcttctt tgattataca aaacccggac cgggaatacc tgaggatgct ccaccgaaag
    15541 cgccaatcat tgtttttttt gagatctatt ttaggaaatt ctggaacttg gttaaaatta
    15601 accttatgtt caacctgttt aatttaccgg cattattagc tgttttattt ggaacaatgt
    15661 atttaataca ggggcagatt acagacgatc cggctatgga tcttatgaca cgtttcataa
    15721 ttggcagcgt agttatatgc ataccgttga ttacagtagg accggctcag gcaggattca
    15781 cttatatact cagaaattac tcaagagaag aacatgcttt cctatggtgg gattttaagg
    15841 atacggccat tagtaatttt aaacaaagcc tgattatttc tgtaattgat tttttcgcct
    15901 ttatattatt tggcatagta gttaacttct attttaagta tactgcaaat aataatatga
    15961 taatgactct tatggtattt gccagaacta ttattatact tgcttttcta atatatttaa
    16021 tgatgcatat gtatatctac ccaatgcttg ttacatttaa gttaaattta aggcagcttt
    16081 acagaaatgc tttaatcttt tctcttgcaa agtttttccc caatttggga atattgatat
    16141 tatgtacagc actagtactt ttatctttct attttaacac tcttatagga attattttgt
    16201 ttattttcct tacggttagt actataggtc ttattattaa cttttacatt taccctaaac
    16261 ttaaaaggta tatgatagat aaggttgctg atgaagacac agaagttgat gaagatgaag
    16321 attacgaaga tgatgttgac gatgttgaga aatctgataa tacagaggaa gataataata
    16381 gaatagaata gaatatcaat catatgaaaa aatgaagatg accggatatt aaccgatgtt
    16441 aacaggatga taatttaaat ggcaattatt aagtaagaat tatgttgtaa aaaaagtcag
    16501 cataaaaaac agcacaatat cagattgtgc tgttttttta taattgtatt actactttgt
    16561 tatgttgcca atgggaaacg cattccaaga gtgtaacttt ttgatgaccc gtaaagaata
    16621 cattgctttc caaattgtca tagcagtatt attttgatgt tttaattgtt agagagctat
    16681 tatcctgttc tatcatatca tgcattgaat aaagaccggg ttgcttatta taaataaata
    16741 acgctgcttt tatggctcca gtaccgaata tttcctttga catagcagtg tgttttaatt
    16801 cgattatttc atcattacct gcaaaaataa cagagtgctc acctacaatc gtacctcctc
    16861 ggacagcatg aatcccaatt tcggttttgc ttcttttttt cctgcgggca tgcctgtcat
    16921 atacatactc ctgtttttgt cctatgacag tatttatggc atctgcaatt gccaaagcag
    16981 taccactggg tgcatccagt ttttgattat gatgtttttc aattatttct atatcaaaat
    17041 agccttcaag aagtttagag gcctttttta caaggtctat aagcaagttt acaccaagag
    17101 acatatttgc tgattggaaa actggtataa agcctgatgc ttctttaagc ttcttcatat
    17161 gaagatctga taaacctgtg gttgccataa ccagaggtat tttttttgaa actgcatatt
    17221 caagtaaatt atctagtgct gcaggattgg aaaagtcaat aatagcatca acatgaatat
    17281 tgcagtttcc aatattcgta aaaacagggt attgattttt aattgaatca tttatatcgt
    17341 aaccagcaac aatttccaag tcagtgtttt gctgggccag tctggtaata acctgaccca
    17401 ttttcccatt acagccgctt aacagaattt ttatcatttg cattcattcc tctctttaac
    17461 tcagttacat caaaccatat ttcgttagtt cggattttag tatttccaga tttttttcgc
    17521 ttatatccac caaaggcatt cggcattttc cgacattcat tcccataagg ttcattgcag
    17581 ctttaattgg aataggactt acttcgataa acaaagcttt gataagattc agtgttttca
    17641 attgtatttt acgactgctt tcaatatcac cttccagata tttataaacc atgtcatggg
    17701 tgtcttttgg tataatattt gccataacag aaataacccc tttacctcct aaagctagta
    17761 gaggcactac ctgtccatct tctcctgaat aaattgtaaa atcatctcca cagagattaa
    17821 ttatatctcc tacctgggaa agattacatt cttttattcc gtaaatatta tgaatctcag
    17881 ataatgcttt aatggtatca ggattgatat tcaagtttgt tcttgatgga acattgtaaa
    17941 gaataatagg tattttaata cttgaagcga ttgctttaaa gtgttcgtac aatccttttt
    18001 gagttgtttt attatagtac ggtgtaacac taagtatacc gtctgctcct gcatcttcag
    18061 cagctttgct aagttctatg gcatgacggg tatcattgct tcctgtaccg gcaataacag
    18121 gtattctgcc atttactttt tctacagtaa atttaatagc tgaaagatgt tcttcatcag
    18181 gcattgtaga agcttctccg gttgtaccgc atgatactat ggaatctata ccttggttaa
    18241 tctggaactc aattagctcc tcaagttttt caaagtttat gccatctttt gtaaaaggtg
    18301 taataatagc aactccagcg ccggtaaata ttggtttcct cataaatata catcctttct
    18361 atattatttt gcaatgtatt tatatctgtg gttaatctta ttttagattg attttatatt
    18421 acaactccat tatattatat gtcaaataga aatttccagc cattaataat tttattacaa
    18481 aaaaattaaa ttttatatct ttaaagggaa aaaagcagtt attgtcgaat gataaaataa
    18541 atataataat tttgcaaatt gagtgtgata aggtggtttt tatgcaaaag acaaagaaaa
    18601 agataatatc aataataagt aaattaatgg tgacttgcct ggttgtcata ttgatcttga
    18661 ttcaagtaca ggtaaaagcg gaatcatcga ataataatgt atttgatgaa gtaagagtag
    18721 gaatgttttt taagaattct tcgtcaccta atgtttcaaa tgaaatatca tattttaaca
    18781 tttcatctcc taaaggcgtt gaagcagggt atttaaagga tggtgaattc ttcaaaatat
    18841 ttgatactgg tgatggttct gtattaacag ttcgcaaaga ttcatactac attattgatg
    18901 gcgcttcaat acaagaatgt aaacctggtg caaataattt accagaagag agtaaaattg
    18961 gtcccttcca tattcaggtt ggaggttctt atgataacta tgaaacagtt aaaactgaag
    19021 tagataaata catacaaaag ggtataaatg tatatccggc ttatactgat tcatggtata
    19081 tctggacagg tttttatgta gaccaaaatt ctgctgagca aaacattaaa aattctctca
    19141 ttcctaaaat cggtgaggag aaatacagta ttgtacaacc ttcaaaaaat aatattgttg
    19201 ttaccatgaa atctaatgaa accagactga tatttggaaa tagtgaaggg tattttcaag
    19261 ttaggccgcg gatggaaaac aatccgtata tatttaatat taacggaaaa aattatagag
    19321 gaataattga aacaagaaga ttggaagaaa gtgatttaac agtaataaat atactcccta
    19381 tagagcatta tttatatgga gtggtaccat ctgagatcga atggtcgtct caccctgaag
    19441 cattaaaggc tcaggctgta gcagcaagaa cttatattta taataatatg ggaaaatact
    19501 caaaactggg atttgatgta tgtagcagcg taaattctca ggtttacgga ggatttagcg
    19561 tggaaaaaga aacaactaat aaagctgttg acgaaacgca gggtaagaaa ttatactata
    19621 atagtgttct tgcccaggtg ttttacttca gctcaagcgg gggaaggacg gaggctgcga
    19681 aaaatgtatg gtctgaggat cttccgtatt tgcaaagtgt agaagataaa tatgaacagg
    19741 aaggatcaaa aaactatact tgggaaaaaa caatatcagc agctaaagct ggagatatta
    19801 tggtttcctt gggtaatgat attggtgagg tgttaagaat aacaatcgac aatacttcag
    19861 aagcaggaag agcaataaag atgacggttg ctggaactaa aggggaaaaa gttttcctaa
    19921 attcaagatg cagagatttg ttgagcttgc ccagccaatg gtttacaata tcaacagact
    19981 ctgatgcagc ggtatggaat agttcggcta atgaaaagat tagtgtgcag gttggaggga
    20041 aaaaggtagt ttcagtaaac ggttcaggta ctatagctgt aaatggaaac atttcagtac
    20101 tgggagataa tggattaaaa aggataatac cagcagttcc ttctacatat acctttagcg
    20161 gcagaggtta tggacatgca gttggtatga gtcaacaggg cgcaaagggg atggcaaatg
    20221 caggtttcac atatgaagat attttactgc attatttcta cggtacaaat attaaatatg
    20281 agtagtttga agagtgcagt taaattaaaa tagcaggtta ggaatgatat tatcaatgaa
    20341 ggttaaagat ttttactatg agcttcctaa ggagttaatt gctcaggaac ctctgaaaga
    20401 aagaagttca tctagattaa tggtactgga aaaaagtacc ggaggaataa cacatgaaat
    20461 atttaataat attgcaggat acttaaagca aggggattgc cttgtaatta atgatacaag
    20521 agtaataccg gcaaggcttt taggtgaaaa agaggataca ggcggaaaga ttgagtttgt
    20581 tctgttaaag aaagaaagag atgatatatg ggaagttatt ctaaagcccg gcaggagagc
    20641 aagaccgggt tcaagatttg tttttgggga tggcattctt aaagctgaga ttatcgatat
    20701 acttgatgaa ggaaatagac tggtaaaatt cgaatttgaa ggaatatttg aacaggtact
    20761 ggacaaagtg ggtatagtac ctcttcctcc atatattaca aaaaaacttt cagattccga
    20821 aagatatcag actgtatatt caaaatacag aggctcagca gcggccccta cggcaggatt
    20881 gcattttaca aatgaattaa ttgattcaat taaactaatg ggagtggaag tagccaacat
    20941 tactttacat ataggattag gtactttccg cccggtaaaa gttgataatg tggaagagca
    21001 tcatatgcat tcagagtatt acaaaattga ccaggaaacc tgtaataaaa taaatgctgc
    21061 aaaaaaatca ggaggaagaa taattgcagt tggaactaca agctgcaggg tattggaaac
    21121 agcttccgga caggatgggc tgctaaagag cggggaaggg tatacggata tttttatata
    21181 tccaggttat aaatttaaaa tgattggtgg ccttataacc aattttcatc tcccggaatc
    21241 cactttaata atgcttgtta gtgcctttgc aggttatgat aatgtgatgg aagcatataa
    21301 gactgctgta aatgaaagat ataggttttt cagctttggg gatgcgatgt tcataatatg
    21361 atttatactt ttttaaaact tattatacct taagtatcag aggttagaga ggtacaaata
    21421 tgtcagctgt aaaatatgaa cttataaaaa aatgtaagca aacaggtgca aggctggggg
    21481 tggtgcatac acctcacgga agttttgaaa cacctgtatt tatgcctgta ggtactcagg
    21541 caacagtaaa aacaatgtca ccacacgagc ttaaggaagc aggtgccaaa atcattttaa
    21601 gcaatacgta tcatttatat atgaggcctg gacatgatat tgtaaaagag gccggaggac
    21661 ttcacaagtt tatgagctgg gacagttcca tattgacaga ttccgggggc tttcaagtgt
    21721 tcagcctcag caacctcaga gatattcatg aggatggtgt atactttaaa tctcatattg
    21781 acggttcaag gcattttata tctccggaaa agtccatcga gattcagaat gcacttggct
    21841 ctgatattat tatggctttt gatgagtgta taccatatcc aagtgaatac aaatatgtat
    21901 atgattcagt tgagagaact agccgctggg ccaaacgctg taaaattgca catcagaata
    21961 cagaaaaaca gtctttgttt gggattatac aagggggcat atttagtgat ttacggcaga
    22021 aaagtgcgtg ggaaataatg gatatcggtt tcccgggtta tgctttgggt ggcttaagtg
    22081 ttggtgaacc tgcagaagaa atgtatgaaa tgcttgaatg tacactgcca ttattgcctg
    22141 aagataagcc cagatatctt atgggtgttg gaagtccgga ctacctaatt gaaggagcta
    22201 taaggggtat tgatatgttc gattgtgtgc tgccaacccg tattggaaga aacggtactg
    22261 tccttactag caggggaagg gttattgtga gagatgcagc atatgcaaga gacttttcac
    22321 cattggatcc ggattgtgac tgttatacat gcagaaattt tacgagagca tatataagac
    22381 atcttttaaa aactaatgag gtccttggta tgcgacttac tacataccat aatattaaat
    22441 ttttaataga cttgatgaaa aagataagac aggctataat agaagataat ctcggagatt
    22501 ttagaaaaaa tttctttaat tccttcgggt atacaaaatc aacaaccaat atttagattt
    22561 tatcaaaatt gtaagaagga ggatggtaaa atgccggaag agggtgctgc aggtttggca
    22621 ggtttgggag gcgttggatt tatactgtat atagtattta ttattggtat tatgtactta
    22681 cttgtttttc taccacaaaa gaaaagagat aaaaaagcaa aagaaatgat tagttcttta
    22741 aaagtaggag ataaggttgt aaccattggt ggattagcag gaaaaataat aaatattaaa
    22801 gatgacgaag ttacagtaga aacaggggtt gaaaaaacca aaatgacctt gaaaaactgg
    22861 gccataaaag aagttgataa acctattgaa gcatagtttg aaaaaattgc ataaatttct
    22921 ttcaaatatg catgtgcata ttttttaagc aggaatattg aaaacaacag attgcacatg
    22981 cggaaaaaat gagaataagg tggagcattt ccaggaaaat ggatggcttc acctttattt
    23041 gttggaggtc agaggtcgga gattagagat tagaggttga aggttagagg ttggaggttg
    23101 gaaatgtcat gtgataagtg aaaagttgtt ctaatattcc tgccattcaa atagattata
    23161 ttaggaatat tttttgggcg ggggtatgtt atgttaaaaa agtctttgca aaaaaaaagc
    23221 agtagttcag acgaacaatt aaaactgact attttattaa aaggcgttat attagcctac
    23281 ataataacaa tacctttttt tattttattt tcccttatac tttcttacac taatttcccg
    23341 gaaaattaca tatctactgc agttacaagt gttactataa ttagtgtatt aatatcaagt
    23401 acatctgttt caagaaacat aaaaaacaaa ggcttgttta gcggtgcatt tattggcttt
    23461 ttttatatgt ttatactata cattattaat ggtatcgtat ttaaaaacta ctccataacc
    23521 agacatttaa taactatgac atttatttgc atactaacag gttctgtagg gggaattatg
    23581 ggggttaata ttgggacagg taaaaggtca aggataaagt caagacccaa gcggggaagg
    23641 gtaagatagg tagaagggaa atcgcaccct tttttcaaaa aatgcttttc aaaactgaat
    23701 ctctatgcta caataaatgc gattaagcaa ataaatttta aagggggcaa atataatgaa
    23761 gcatataaaa actataagtg cttccgttct taaaagatca gctaagaaca gcggatgtgg
    23821 tgaatgtcag acatcatgcc agtcagcatg taagacatca tgtactgtag gaaatcaaaa
    23881 atgtgaaaaa aattaacttc gaaagattaa tgagtaaaat tttttagaag acaggaggtt
    23941 tgtggacctc ctctaatttt gtgtaaattt aaggagaaaa aacatgaaag aaggttttat
    24001 acacaagttt tcgcttaatg ggacgaatac tgttgttgat gtaaatagtg gttctgtgca
    24061 tatttttgac cccgttgcct atgaagttct tgactattat gctgaagatt ttaaggaaga
    24121 agaaataata gcaaaattgg gtgataagta cgggaaagaa caggtacacg aagcttttaa
    24181 agaaataacc cagctaaagc aagaaggaat gttattttca gaagatgaat accacttttc
    24241 tgttgcaaaa aacaatgata tacagtttaa agcaatgtgt ctgcacgtgt ctcatgattg
    24301 taacatgcgt tgcaggtact gttttgcatc taaaggagat tttggatccg gaagaaaact
    24361 tatggatgtc gaaactggca agaaagctat tgatttcttg ataaagaatt ccggtaaacg
    24421 tagaaatctc gaagtggatt tctttggtgg ggagccctta atgaactttg atgtaatgaa
    24481 agatatcgta tcctatgcca gagagaagga acaagaatat aataagaatt tccggtttac
    24541 agtaaccaca aatgcattgc ttttagataa agacaaaatg gattatataa atgagaattt
    24601 tgttaatgtg gtattaagtc ttgacggtcg aaaagagata aatgataaaa tgcgggttag
    24661 ggttgataat tcaggaacat actcacatat tatacaaaaa ataaaggata tggctgaatc
    24721 ccgaaatcag gaaaactact atgtaagagg cacttacacc agggagaata ttgatttctc
    24781 caatgatgtt ctgcatcttg ccgatgaagg gttcaagcaa gtttcagttg agcctgttgt
    24841 tgcagcagcc ggttcaggtt acgatatcag agaagaggat ctgcctgaaa ttaaagctga
    24901 atatgaaaaa ttggcccttg agtatgtgaa tagaaacaac tcaggcagtg gatttaattt
    24961 tttccacttt atgattgacc ttgaacatgg accttgtgca gaaaaaaggt acaaaggttg
    25021 tggctccggt tttgagtata tagcggtaac acctgagggg gatatctatc cttgtcatca
    25081 gtttgtagga attgatgaat ttaaaatggg aaatgtttat gatggaaata tagatgataa
    25141 aatagtctgt actttcaaaa attgcaatat tacttcaaag gatgaatgca gcagatgttg
    25201 ggcaaaattc tattgtggtg ggggctgtgc tgctaatgca ttcaatttta ataaagacat
    25261 aaacaaacct tataaagttg gatgtgaatt ggaaaaaaag agagtagagt gtgctttgtg
    25321 gataaaaaca cagatacatt cactttagaa tgagttacca taagtaaaag gaaatacctg
    25381 tgaatattac tatgtgtgtt agattgcttt atattacaaa ttaaccttaa tatttcaaag
    25441 ggctgaagaa tgataagtat tgactgcatg tcctcatagt tatataatat cgacatatgg
    25501 acatgaaaca aagggggaat taattaacat gagaagaagc agctgggtcg gttttgtgct
    25561 tgtagtagta ataatagcgc tattggcttt tcttgccctg gatgggggaa atgcaatagg
    25621 aataaatggt attagtaaaa ccaggactgg aattgatatt aaaggtggta tatatacaac
    25681 tctttatccg gatcttcctg aaggtcaaaa acctactgaa gaagatttga actcagccag
    25741 gatgataata gaaaaaagac ttgaaagcaa aggtatttat gataagaatg taactgttga
    25801 gaaggaacat ggcaggatta ttgtagaaat tccatttgcc ccgggtgaaa aagactttaa
    25861 ccctcaaaag gctgttgatg aaatcgggaa aacagctttg cttactttcc aggaagtaga
    25921 tgaaactaaa gtagatgaga aaggcgattt ccttcctaca ggtagaattg ttatagaggg
    25981 aaaggatgta aaaactgcgg gtgtagcaac aatgcctgat actggtgaaa ttgttgttac
    26041 tcttgaactt aacaaggagg gtgcagaaaa atttgctgaa gcaactaaaa gattgcttgg
    26101 taaaagaatt gcaatcttta tggacgatag ctatattact gcacctgtgg ttaatactgt
    26161 aattcccgat ggaagtgctc aaattaccgg acaaagaact gcaaaagaag ctggagagct
    26221 tgcagataca attagagccg gtgctttgcc tttcagactt gtagcaaagg aattaaactc
    26281 agtaagtccg atacttggag aaggtgccct gaaagtaaca atcaaagcag gaatagtagc
    26341 atttatactt gtagctgcat ttatgattgc atattatagg cttgccgggc ttatagcaga
    26401 tattgcttta tcaggacttg ttgtaacaac attgttatta atttctgtaa caggtaatac
    26461 attaacactg cctggtatag caggtataat tctttccgta ggtatgggag tagatgcaaa
    26521 tgtcgtaatt tttgaaagaa taaaagaaga acttaaaaat agaaagacca taggagctgc
    26581 ggtagatgat ggatttagaa gagcatttac agcaattctc gattctaacg taacaactat
    26641 aattactgct gcagtactat actggcttgg ttcaggtgct atcaggggat ttgcacttac
    26701 tcttgggatt ggtgttgcaa taagctttct taccggggtt actgctacca gagttatgtt
    26761 aaaatctata tgtagcgcaa aatttgccaa gaatcccaaa ttatacggag cttaaaggag
    26821 gtgccttaat gatagattta atgggaaaaa agaaaatatt ttttgctata tcaatctttg
    26881 ttattctagc aggtgtagta ggattaatag taaatggcgg cttgaattat gatattcaat
    26941 ttcagggcgg caccattata caggtagaaa tgagtgatga taattttgat gccaataata
    27001 ttggttctgt tgtaagtgat atgcttaata aaaaggtatc atcacagaaa ctaaaaacat
    27061 ataaccctaa gaatgaggca gagagcatta atatattaat gcttaaggtt ccaagcgacc
    27121 atacattgag tgatacggaa attaataata ttgtggactt attgagaaaa gattataatg
    27181 taaatcctga agctgagata cagatgcaga gcgtacagcc atctatagca gcagaaatta
    27241 aacaaaaagg cattacagcc atactggtat catcagcatt gataattgta tatatatggt
    27301 ggaggttcag taacatatca ggactgccgg ctgctataac cgcagttatt gccttgatgc
    27361 atgatgcact tgtaatgttg tctgcatatg ctatttttaa gataccgtta aatgagtctt
    27421 tcatagcagc agtattaaca atactgggat attcactaaa tgatacaatt attatttatg
    27481 accgtataag ggagaacagc ggcaagttga gaaaacttcc tgtgaacgag cttgttaaca
    27541 caagcataac tcagactatg ggccgtacaa taaatacttc tgttacaaca cttatagcaa
    27601 tagttattgt atatatattt gcgtcagcaa ataatataca gtcaattaaa gaatttagtt
    27661 ttccgttaat tataggtatt ataagcggaa cttattcttc aattttcatt gccagcccat
    27721 tatggatgat gtggaaggaa cgacagagca aagctaaaat agtcgcaaaa caagctaaag
    27781 caagaacctg atattatata ttatataatg ttatatatat ataatatatt gatattaaaa
    27841 aaatatacta aaggcggtcg ccataataat tatttatatg atgaccgcct taaatgtttc
    27901 atatatgctt tttgctggat tttgatattt actgctattt gttactaaaa gcataatcaa
    27961 ctaacagaca tcctattaca acttctgcca gaatagtaaa actagaatta ataacagaag
    28021 tagcaataaa ctccttataa ggatcatggt taaagacaaa gcggtttaga acagaaagta
    28081 aagtgccgga tgcaaataaa aaaatagaaa tgttagcacc cagttttatg aaactaattg
    28141 tatgtttgtt aagtttacat aattcattat aaattttttt gattgttttg tcgataccta
    28201 gtgttttatt caatttaagc acccttccag atattatacg taataaattt tctcttatat
    28261 gttaacataa aacaatacat acttcaatac ctaatattta ccagagaagc attttctaca
    28321 tttgaagaga aagcacctgt ttgccagaag acaaaataat ttaaataaat aaaggatatt
    28381 ttgctgaata tgtagaatat attttacatt ggttattata attaataact tattattact
    28441 atgccagttt ggaaagagat atctcttccg actagaaatg tactttccat tggcaacata
    28501 gcaaagtagt attaatataa tttcaaatac tatatgatat ttgaatataa ataacaaatt
    28561 aaaatatttg agaaaaaaga aggattattg atatttttgt cgaagaataa tattctatag
    28621 gataatatta attgtgaagg agatgagcgt ttttagtaat tgctaatgta taactatttt
    28681 aatgacgaat ttattgaaga aatcagagtt aataatgaca tagttgatgt agtatctgaa
    28741 tatgtacacc tggagaaaaa agggaggtac tattttggtc tttgcccttt tcataatgaa
    28801 aagactccct ctttcagtgt aactccagcc ttacagatat ttaactgctt tggatgcaat
    28861 aaaggcggca atgttattca tttcattatg aatattgaaa accttgattt tgcggaagca
    28921 gttaaatttc ttgcggatag agcagggata aggctgccgg atgaaaaaaa tgtaaacgac
    28981 gaagaatata agcttaaaaa ggatatattg gatattaata gaactgttgc acattatttt
    29041 tatgacaatc tgatgtcttc aagaggtgtg aaaggacagg aataccttag aaacaggggc
    29101 atccatcctg caacattgag aaagttcggt ttgggatatt ctcttcccga aggaaataat
    29161 atatttggat tatttgtgga taaaggcttt agttataagg caataattag tagtggttta
    29221 gtaataaaaa gcaaaagtgg cgaatatttt gacaggttta aaaacaggct gatgtttcca
    29281 atatttgatg taagaggtaa tgtaattggg tttggcggaa gaacgctgga tggctcgtta
    29341 cctaaatata taaactcacc tgaaacatta gtatacaaca aaggaaagca tctatatgct
    29401 ttaaattttg caaaaagcat aaggaaaaaa aatcttattg ttgttgaagg atatatggat
    29461 gttataaccc ttcatcagaa tggaataata aatagtgtag cacctttagg tacggcactt
    29521 actgaaaatc aaggacgatt attaaagaag tatgctgaag aggttattat ttcttttgat
    29581 gcagatactg ccggtcaagc agctgcaata agaggcctgg atttattagc gggtattggc
    29641 tgcactgtaa aagtactatc gatacccgat ggtaaggatc cagatgattt tgtgagaaaa
    29701 aatggtgctg atgaatttag gaaacttata gatggatcaa ggaatcttgt agaatataaa
    29761 ataggttgcc tgaaaaatcg tattgataca agcactacag aaggtaagtt aaaatttgta
    29821 aaaaaggctg cagaaataat atctagactt gatagccggg ttgatgtgga agtatatgta
    29881 aagaagtttt cggaacaata tggaataagt gtagaggcat tttattcaga agtgttaagg
    29941 agtgtacagc ctggtaaaaa tattaaagag gtgaaattct ataatgaaaa aaaacataat
    30001 gatgttaatg cagcatcagg taagaaggtt ggaaatattg atttaaaggc caattatagt
    30061 gaattgttgt tatttgctat tctttgtatt gataataatg cctataaatt gattaaggat
    30121 aaaattggag tggctgattt tagtgcagaa aataagccaa ttgcagagat agtgtttaac
    30181 agactgcaat ccaattacgg aattgtatct gcagaactat taaacataat ggaggctgag
    30241 aaagcagggg agtttgcaag aattcttgag caggagtgta atttcgatga tagcagaaaa
    30301 gctgctatag atgtagttaa taaaatattt ataacaaaac tggagaacag aaagcgggaa
    30361 atactggaaa tgttgaaaaa taaagaaaat atgcaaaatt tacaaacaag tgaattgcta
    30421 aaagagttaa atgaattggt agttgtaatc aaaaagcatt ctgagtattg atgaaaggag
    30481 ggtaaatttt gaaagttaat aatgaaacaa gaaaaaccat attaaaagaa ttgattgaaa
    30541 aaggtaaatc aaaaggcatg cttacttaca aagaaataat ggatgccttt gaagaaattg
    30601 agttagatcc ggaccagatt gataaaatat atgaaacagt agagaatatg ggcattgata
    30661 ttgttggcga tctggaagag gatgttgaag atatccagat aaacgaagaa gatttggatt
    30721 taagtgttcc tgaaggaatc agcatagacg atcctgttag aatgtatctc aaagaaatag
    30781 gcaaagttcc tcttttaact gcagatgaag aaatagagct ggccaggaga atggaggaag
    30841 gtgataatga ggcgaaacga aggttagctg aagcaaattt aagattagta gtgagtatag
    30901 caaaaagata tgttggcaga ggtatgcttt tccttgacct tatacaggag ggaaatcttg
    30961 gtcttataaa agcagtagaa aagtttgatt atagaaaagg ttacaaattc agcacatatg
    31021 ctacatggtg gatcagacag gcaataaccc gtgctatagc tgaccaggca cgtacaataa
    31081 gaattccagt tcatatggtc gaaactatta ataaactcat aagagtttca agacagttat
    31141 tacaagaact ggggagagaa cctttaccag aggaaatagc aaaggaaatg aacatgcctg
    31201 tagaaaaggt gagggaagtc atgaagatat cccaagaacc ggtatctctt gaaacaccta
    31261 tcggggagga agaagacagt caccttggag attttattcc tgatgacgat gcacctgcac
    31321 ctgcagagtc agcagctttt actttgttga aggaacagct tatggatgta cttgatactc
    31381 ttacacctcg tgaggagaag gtattaagat taagattcgg actggacgat ggcagagcaa
    31441 gaacactgga ggaagtagga aaagagttca atgtaaccag agagagaata aggcagattg
    31501 aagcaaaggc attaagaaag ctcagacatc caagtagaag taaaaaactc aaggattatc
    31561 ttgattaaga ctcgattatt aaatcgagtc ttctcacatt catcatccat gattcaggtg
    31621 acggctcacg acatccgtgt cgtgaattac gaattttccc ttgccttaaa cataacaatc
    31681 cgtaaacttt gttcaactta tatactcaga aaatcatttg ctgccatatt ttgtttcatg
    31741 ggaagaaaat gcgccctgca aattgcacag tgttgactgc cgaatgattt attgcaccag
    31801 aatcaaattt ctataatttt aaaaaatcaa gctataatta acaatcaaac gaaatattaa
    31861 gtaatgcggt cataacttat atacaaaggc ttagattgga aaaataaatc ttgaccacat
    31921 atagcggcgc aagtataata ttatttgtca cagttgagac attcctcaat agctcagtcg
    31981 gtagagcatg cggctgttaa ccgcagggtc gtaggttcga gtcctacttg aggagccaaa
    32041 agggccttta gctcagttgg ttagagcaac cggctcataa ccggttggtc cggggttcga
    32101 gtccctgaag gcccaccaaa tattgaaaaa agtaaggaac ctattcctta ctttttttgt
    32161 tataatgaat aggaatgtat aaattttccg aatgtgcact tttcatgtag actgttttaa
    32221 aaatcattgt aaaagttaaa tcaaaggact agtttgaaga aattaaaatt ctggcaatct
    32281 aatatgatag caggaatgga gctttaaata tgaaacttaa gggaagatta agagcgatag
    32341 ctgaaatggt tcctaaatgc aatttgatat gtgatattgg caccgatcat gcttatatac
    32401 ctatttatct tgtaaaaagc agtatatgta aaagagcaat tataaccgat attaaacagg
    32461 gacctattaa tatagcagaa aataatatta gggaaaatca tttggaggat tgtatagaaa
    32521 caagactcgg agatggactg caccctttag ataataacga aatagatgtt ttaataatag
    32581 ccggtatggg tggaaaactg atggcaaaaa tactatggga aggtatatcc aaagcaatga
    32641 atgcaaacct tttaatattg caggctatga acggattgga gcttgttaca caatggcttt
    32701 tcgaagaagg ttttgatata tgtgatgaag agttagttga agatgataac aggatatata
    32761 atataaaggc tgctagatgg acaggtgtta aaaaagtata taataatgtg gatttacata
    32821 taagcaaaaa acttattgaa aaaaataatc cattgcttat tgagtatata gatagaaaat
    32881 tggcagtatt ggacaaaatt atttcgggat tgcagaaaac aaataaaaat tcagcagaag
    32941 cggaaaccga attgtataaa atggaaatac ttaaaaagga tatgacggaa ataataaata
    33001 atatgaggtg aatatcatga gtgccactaa atgcaaggga atagttgatt ttatggaaaa
    33061 cattgctccc gtatatttag cagaggattg ggataatgta ggcttaataa tcggagacta
    33121 tgaacgtgat attaatcata ttctggtcag ccttgacgtt acaactcaaa tattggacta
    33181 tgctatagaa aaaagtgttg atatgataat atcccatcat cccctaatct ttaaacccat
    33241 taaacgaata gtatacgatg actggaaaag tgttttaata tcacggataa taaaaaatga
    33301 tatatgttta tactgcgcac atacaaacct tgactatgca aaaaatggag ttaattggaa
    33361 tttggctcta gctttggacc tgaatgatat taaaaattat ggagaaacta agtcagaagg
    33421 tgagggatat agccttgcaa aagttggtct gttaaaaccg cccatgaaat tatcaatgtt
    33481 tgtcagcaat ataaagcaca tactaggtat tgattccgta aaacttataa gtaataatgc
    33541 tgacaaagca tttgaaagag atgtcaataa agttgtggtg ttttccggaa gttttgatga
    33601 tagtgttcta cgctatatag gtgatgatat tgatgtaatt ataactggtg atgtaaaata
    33661 ccatactgct gttgaaatag cggaaaaggg tgtttgtgta attgatgcag gccattttgc
    33721 tacagaaaat gtcatagttc ctcagctttg ctctcaatta aaagatagat ttcctgattt
    33781 aagtgttaca agtaaaatgg tggtaaatca gccaattaag ttttattgac aatagatata
    33841 attgagctta taattaaaaa gtcagctttt atgaattaat gtgaatacat attagtgagt
    33901 aagccagacg gccgcgtatg taatgctgaa aagctgcata tgaggaaagt ccgggctcca
    33961 tagggcaggg tgccgggtaa cccccggtga aggcgacttc aaggaaagtg catagaaaat
    34021 aaccgcctga ttttgtgtca ggcaagggtg gaaaggtgag gtaagagctc accagcggtc
    34081 aggcgactgg ccggcttaga aaaccccacc tggagcaaca ccgcaaagtg ggacgttaag
    34141 gtggcccgcc tgtcccgggt aggtggcttg agccatacag taatgtatgg cctagataga
    34201 tggctgttta atacagaacc cggcttatag acttactcat aattaattat caaaataccg
    34261 gcttaaaaag ccggcatttt gacctatttt acatttctcc agtcttcatc caatccaagt
    34321 ccattaagat ctgcaataat gtttttcgtc tcagcttcgt acatacgcca gattattctc
    34381 aaaccctgca aacttgccgg gccatcagta aatggtcttg tatcgttttg tatgcaattg
    34441 aggaaatgta gagtttcaaa ttgtgttttc tttgattgat cagtttccac cattagtacc
    34501 tgagttcttg aagttgtgtc caggtttgcc ttttccccct tgatgtggga atgaaggtat
    34561 aatttacctt ggccgtcagc tttgctgtac tccagcatac cctcggtaca gtggatatga
    34621 aagctgtagc caagccgtgt accccgtgca ccccatgtac caaaatggta gcccattgca
    34681 ccgttctcaa actctattac ggcattactt gtgccttcat tttccatcca tggtgtacca
    34741 aagtttgtac ccatatgaaa tcctttcact ggttttccaa gaaaccataa caaaagatct
    34801 atatagtgac acccatgact gaaaaactga cctccaccaa gcctttttgc agataaagcc
    34861 caatgccctt ccgggtattt agtgaactgt tcagtccaaa tagacatttg aaatgcttca
    34921 ccataagtct tagcatctac cagttccttc atctttaaaa tgattggcca gtaacgtaca
    34981 ggataagccg tcattagcac ttttttatac ttctctgcag tttcaattaa cttaatgcat
    35041 tcctcttctg tgttgcacat aggtttttcc attaatacat gtttacctgc cttaaggaaa
    35101 aacattccag tttcaaagtg caagtcatgc ggcaatgaga ttagtacagc atctacatta
    35161 tttaccattt ccctgtaatc agtgacagct atttcagctc caataacctt tgcagcttcc
    35221 tgaacccttt caggtacaat atcacaaact gcagtgacaa ccatcttgtc actcaattcc
    35281 aaaagtccaa gcagatgaga ttttgccatt ccaccgcaac caataactcc catacggatt
    35341 ttttccattt attattccac ccccaagata ttggatatat tttttatgct agtactactt
    35401 tgtcatgttg ctaaatgata taatatactt aaattattta aaaatctttg ctagtatgtt
    35461 ctaataattt gcaatattgt acaaaaggtg ggtgaagctt atggttcggg caagatacat
    35521 tcttcaaaat agcctcacta ttgataaact aattactttt tattatatgg aattaaccag
    35581 caaatttatg acaagaggag aaagacatga tttctgggag tttgtttatg ttgataaagg
    35641 tgaaatagag atacttaccg attctaaaca atatgagtta aaccagggtg atattatatt
    35701 ctataaacct aatgagtttc atgcaggcag ggcaaaaaac aatacaaatc ccaacctaat
    35761 tattatttcg tttgagtgtt ctgcaccatg tatggaattt ttcgagggaa aaacatttcg
    35821 actggatgaa gaagaaagga aaatcttatc aaggctgatc aaggaaggtg ttggggcttt
    35881 tgatccacct gtagattctc cgaatatgag attgcccagg agaagacaaa atgcaccatt
    35941 tggaagtgag caattaatta aaaaccacct tgaaatcttg cttattctaa tgattcgcaa
    36001 gggtgataaa agacaagaaa atgaaagact tcaaacaatt acctcggaga atagagaaga
    36061 agatttgaca gcgcaaatta aagagtatat gaaaagctgt atttgtgaaa acttaacccc
    36121 tgaacagctt tgtactgcat tcgctataag taggacgcag cttatgacca tgtttaaagc
    36181 aaaaacagga ttgggagtaa aagagtattt taacaagctt aaaatagaaa aagcgaagga
    36241 aatgatccgt tcaggacaat acaacttttc tcaaattgcc gggtaccttt cttataatag
    36301 tgttcactat ttttcgaaac agtttaagtt tataacacaa atgacaccta cagaatatgc
    36361 acgttctgta aagtcacggt agaaaattaa tattaagatt aaaatttcaa actattgaaa
    36421 taatattaat agaatactac tatgccaatt tggaaagcaa tgtcttccga gaatatgctt
    36481 tatattagca ttggcaacat aataaagtag taagtagtat aaaaatagaa aatttatggc
    36541 tggccgggga gggtggcata ttggcagaag aaaacaaggt aacctttact aaacattaca
    36601 gtgaaaatga aaatacatgt gaaaagagat ataaaggaac gtatggagat gtctgcgata
    36661 atgatgaaac agcagaaaat gagacatcga ctgatattga taggacaata ggggcagcaa
    36721 aagtatccaa tcccagggga aatattcatt gccttactat aattggacaa atagaaggcc
    36781 atattatatt acctccacaa aataaaacaa caaaatatga gcatataata cctcagcttg
    36841 ttgcaattga agaagatgac gaaataaatg gactattatt aatattaaat acggtaggtg
    36901 gtgatgttga agcaggactt gcaatagcag aaatgatagc aagcatgtca aagcctactg
    36961 tttcgttagt gctgggtgga ggacatagca ttggtgtacc tatggctgta gcaacaaatt
    37021 actcatttat tgcaccatct gcaacaatga ccatacaccc tctaagattg aatggtttaa
    37081 taattggggt accacagact tacgaatatt ttgatagaat gcaggagaga gttgtacaat
    37141 ttgtatcaaa aaattccaaa atcagtcgcg atgagttcag gagtttgatg ctaaaaacag
    37201 gtgaattggc aaatgatgta ggaacagtat tatttggaga agaagcagta caaaatggtc
    37261 taataaatga attaggcgga ctttccaggg cgttggaaaa gctatatgag ttaattggca
    37321 tgagacgtaa acaagttgtt ttaccaaacc aatccacaaa tattaattag taatgcaaat
    37381 atggaggtat aacagttaat gatactttac tcaataatac cattagatgt agtatttgga
    37441 aacaataatg aaaagtttgg cgaattattt gaaattgaat atatgggtga gaaagtacag
    37501 gtatattctt caacagaaga tgaatatgta ataaaccggg taatcagtac atcgccaaag
    37561 gcttttctta gagcagaact tcagccggga tcttttatta atgctaatat tgctgatatt
    37621 agggttaaaa gtaaagccac taatgcaaga ggattcagta tctatcataa atagacatta
    37681 gcataaaaag aatcatccca actgtaaaaa tatgggatga ttctgagcat ataagcattg
    37741 aaatttatat aaatattgat tgtaaatgtg aatgatgtat ttagttattc aatcttaaat
    37801 ttattgattt cctctaatag cttagaggct gcatcgttaa gatcttttgc ataattagcc
    37861 agttcttcta tactagatac ctgttcttca attgatgcag tcacttcctg tgaataggcg
    37921 gctgtttctt cggatacagc agaaatactc tgtatggaat tcattgtgtc ttccttgttg
    37981 tgttccattt tagttatata ttctattatt tgtctgatct tttcaactaa taattccatt
    38041 gatgaagata ttttctcaaa aacagcagtg gttccaacaa cagcctcatt ctgtgagttt
    38101 attactttac cggctctttc agctctttct acagtttgaa tagtttgctt ctgtatggat
    38161 tttattaaaa cggaaatctc acgtgatgca gacatggatt gctcagcaag ttttctcacc
    38221 tcagttgcta caactgagaa acctctgccc atttcacctg ctcttgctgc ttcaatagcg
    38281 gcatttaatg caagtaaatt agtctggctt gcaattttac ttattacatc aacaatttca
    38341 ccgattgatt cggaattttt cccaagaaca tcaatatcct gtaatataga atttgaaatt
    38401 gatatagtct cacttgactt ttcagtaaga tcttttatag aagacaaacc ttcccttgta
    38461 aaatccaatg tttttaatga gaaatcttca attgctttgg cattttcaaa tacattattt
    38521 atttcattag ccaattcgct catcttaatt gtactctgtt cagcatcact agcctgggac
    38581 gaggctccct gagcaatttc ctgaacagcg atagatattt cattggatac ggaagaaact
    38641 tcctgagctg ttgtagaaac agtgtctgca gcagtagaaa ctgtccgggt aagtcccatc
    38701 acttgtttaa ttagtcccct catattgccc agcattgacg atatattacc agccagaaaa
    38761 cccagctcat ctttgcgatc cgactctgtg tctactgtca gattgcctgt agatgcctga
    38821 ttagctacta tgttaatttt atttattata ttcccaattc ccatagccat aaaaactcct
    38881 attgcaagag cacataacac tgataatata actaataaca ctgtagtttg cgcaattttt
    38941 cttgaagaag aaagcaatgt agattccggt ataaggttga aaagtatata tccggattca
    39001 ccgatctttt gatatgacat aagatatttc tttttactga cagaaactat tgatgagcct
    39061 ttagcttctt cactttttat tatttgcttg taaaatggct gctctgttat attaatttgg
    39121 acaagagttt tatccttttt actctggtta tcttcacttt tatctgtttt gttgacattg
    39181 gcttcattgg aaacttcttt attaacagaa gaatattgat aaccatcggg gcttactata
    39241 aaaaattcat catcttcact gaacctcata tcatcgaaca atgaatcaac aatatttata
    39301 tttaaatcca acattaagaa acctacaggt tttgaaaggg tcatgctttt aactattctt
    39361 actgcactca gagagaaatc taaaggtacg ctgcctgtaa tattatcgat ttcagaatga
    39421 ttgcctgccc ataacagctt tccttcagca gcagctgctt ttttatataa ttcagtatcc
    39481 ttaatggaat caatgttata tccatatgta ttatttatac ctgatgctag tattttattc
    39541 tcatctccaa taagaaggac gttttttata aatttattac taaaagttgt tctgctaaaa
    39601 gacttttctg catcctgtct tatttggagc atatcgaaat cagtctttga aaatctacca
    39661 tctttatttg ttaataggtc ctggatattt tgatctaaat atatttgcat ggaaaaagat
    39721 tctacattag aaaaaagcag atttaaatag ttttgtgctt gtatcattgt attttctgtt
    39781 gatgaggtag caagcttctt aatagatttt gaagatgtag agaatgatat tgctcctaat
    39841 agaaccatag gaataactaa cgccatgaat gctgcaatta gttttgtacg aatgctgtaa
    39901 agtgattttg aatctaaaaa tggtatcttt tttaatacat ttaaaggtac tttaaaaatt
    39961 ttttttaatg gtttcataag taaagataca cctctttctt taaaattctc attatgctaa
    40021 tattgctttg ctgaaaaagc ataattaaag tgttaatatt tgagcactat attatctttc
    40081 ggcatgcaaa aataaaaatt aatacgataa cataaatttc ataccattat ttatggaaat
    40141 tgaaattatt aggcaggttg gtttgtctat ggaaaatata cctgaagtgt gatattattg
    40201 gtaaagaacc attaatggag gtgtaatagt gcagtctcaa aaaaaacaaa agaaaaagta
    40261 catagttaag aagaagcaag caaaaagctc aattaaacag ccaaaacact cgactaataa
    40321 aagcaataat agtgtttata atgaaattac cgagattttg atactttcac ttggactgct
    40381 gatttttctc agctactata tagatgattc tatgggcata ttcggaagcc tggtcaaaaa
    40441 aatcctaacc ggtatggcgg gaataccatc tttcctcata cctctgatgc ttattgtttt
    40501 tccgatactt atgatcttca aaaaagacaa agcaactatg aggaaaaaag gcatatatgc
    40561 ttttatcctt ttatcattag tttgtgcaat atttcattca ggtacatata aatattttga
    40621 ttatgttaat cgttcacctg tagattgcat agttaaattt tacaacgatg gtaaaggctt
    40681 tataggagga ggtgttattg gaggagtcat ttcacttccg cttttattat tattccaaac
    40741 attaggtact attgtaatat taaccacggc tgctataata atgttaatat tgattacgga
    40801 catttccctg cacgaaatta ccagaaatgt aattagttct attttaacag gaagcagaaa
    40861 tctggttcgg ggaattcggg aaggaagaga aatggatgta attgaggaca gtaatttata
    40921 tgaatttggc tcgcatatga attctgctaa tacgaatgaa atctacacag aaggtaaaga
    40981 tgaagggtca ggctttacac ttaatattaa aggcggagag gaagtttatg ttacaggcga
    41041 ttctaacgat gaacagcaaa ctaaagtagt agatattaaa caacatagga aagctaagtt
    41101 cctcacctta aataatgaaa aggcattgga agtggaagta aagcagcaac agtgtggttc
    41161 tgaatttaaa acagaagttc aaatgataga taaggaatca gaaaaatcta ttcctgaaaa
    41221 tgataagaaa gtagctggga aaaatgtccg ggaaatgttt tataaatatc caccaataag
    41281 cattttaaat agcaatcagg agaatgggaa aaatcttaaa aattataaaa atactgcgat
    41341 tgaaggagca aaaaaacttg aagaaactct gaagagtttc ggagtagagg ctaaagttgt
    41401 taatgtaagc atagggcctg taattaccag atatgaaata cagcccagtt ctggagttaa
    41461 agtcagtaga attgttagtc tggcagatga tattgcatta aaccttgcat cttccggagt
    41521 cagaatagaa gctcccattc cggggaaagc ggcaattggc attgaagtac ccaataagga
    41581 agtgacgcct gtgctattga gagaggtaat tgaatcacag gagtttaaca agcacccttc
    41641 aaaagttgct ttttctgttg gaaaagatat ttcaggtgag tgtgttgtag cagacattgc
    41701 caaaatgcct cacatgctta ttgcaggagc tacaggctca gggaaaagtg tatgtataaa
    41761 cagcttgatt atgagtatcc tttataagtc ttctccagat gaggttaaaa tgctcttaat
    41821 tgaccctaaa gtagttgaac ttggagttta taatggcatt ccccatttgc ttattcctgt
    41881 ggtcacagat cctaaaaaag ctgcaggagc cttaaactgg gcggtacagg aaatgattaa
    41941 caggtacaaa ctatttgcag ataagggtgt aagagatata aaaggatata atgaattagt
    42001 ctccaattcc gatgaacagg atcgtcttcc cctaatagtt ataattatcg atgaattgtc
    42061 agacctcatg atggcagcac cgggtgatgt tgaggatgct atctgccgtc tggcccagat
    42121 ggcaagggct gccggtatgc atattgtaat agctactcaa cgcccctctg ttgatgttat
    42181 aacaggtttg attaaagcca atataccttc aagaatatct tttgctgtat catcacaggt
    42241 tgattccagg acaatacttg atattggtgg tgctgaaaaa ctccttggca aaggcgatat
    42301 gctgttttac cctgcaggta ggcaaaagcc tctcagagtt cagggtgcat ttgtaagcga
    42361 taaggaagtt gaaaatactg ttgaatttat taaatcccag tgcaatcacg agtacgataa
    42421 tgctattatt gatgaaattg ataacagctc tgcaaaagaa gaaaatttgg atagcgaata
    42481 tgatgagctg cttccccagg caattgaaat ggttgtggaa gcaggccagg cctctgtatc
    42541 tcttgtacag agaaggttta aagtaggata tgcaagagct gccagaataa tagatcagat
    42601 ggaagccagg ggtatagtgg gaggattcga aggcagtaaa ccaagacaga ttcttattac
    42661 aaaacagcag tggcaggaga tgaaaatgtc agtggaacag ggataacaat cattgccccc
    42721 ttaccttata tcttgacaca tactttaaaa aagtttacaa ttaatctatg ctaagaatcc
    42781 cattaataaa aaaatttggg gaatgaccat tgaaaactga atacagagga ggtgtcggcc
    42841 atttttgttt atggattatt attaggatta ttaatagcaa ttattgcttc atttataatg
    42901 ttccggcttg ggattaatta tagaaaaaag gttgctgagg ctgaaatagg cagtgccgag
    42961 caagaagcaa aacggatatt aagtgaagcc caaaaagcag ctgaaagtaa gaaaagagaa
    43021 atacttcttg aagctaaaga agagatccat aagagtagaa tggaacttga taaggaaatt
    43081 aaagaaagaa ggaatgaaat ccaacgtttt gagagaagac ttgtacaaaa agaggaaaca
    43141 ctcgatagaa aactagaatc tcttgaacaa aaggatgaac tgttaaacaa gaaagttaaa
    43201 gatgttcagg cacaggaaga aaaggtaaca ggagttctgg aaaatcatct taaggaactt
    43261 gaaagaattt ccggcctgac agtggaagac gctaaagaat accttctgaa aagtgttgaa
    43321 agtgaagtta aacatgaaat ggcaattctt gttaaggagc ttgaatcaaa agctaaagaa
    43381 gaagccgaca gaaaagccaa ggaaattatt tcctgcgcaa tacaaaaatg tgctgctgac
    43441 catgtttctg aaattaccgt atcggtagtt cctcttccga atgatgaaat gaagggcaga
    43501 atcatcggtc gtgaaggacg taacataaga actttggaga ctttaactgg tattgatttg
    43561 attattgatg atacgcctga ggcagtgata ctttcaggat ttgacccgat tcgcagagaa
    43621 gtggcccgga tttcactgga aaaactcata cttgatggaa gaatacatcc tgcaagaatt
    43681 gaggaaatgg tagagaaggc caaaaaggaa gtcgaaaata ctataagaca ggaaggcgac
    43741 aatgcaacat ttgaaacagg tgttcatgga ttgcaccctg aactaataag acttcttggt
    43801 aagttgaaat tcaggacaag ttatggccag aatgttttat atcactcaat tgaagtatcc
    43861 aggcttgccg gaattatggc gtcagaaatt ggtgtggatg ttaacctggc taaaagagca
    43921 gggttgctac atgatattgg taaagccatc gatcatgaag tggaaggttc tcacgtaatg
    43981 attggtgctg acctggttaa gagatataaa gaaggaaatg aaattataaa tgcaattttg
    44041 gctcatcatg gtgatgttga accaacatcg ataatttccg tacttgtaca ggcagctgat
    44101 tccatatcgg cagcaaggcc tggtgcaaga agggagacct tggaatcata tataaagagg
    44161 atagaaaaat tggaggaaat agcaaattcc tttactggag tagagaaatc atatgcaata
    44221 caggcaggta gagaagtaag gataattgtt aaacctgatg atgtttcgga tgaagaagta
    44281 tatttaaagg caagggatat agttaaaaaa attgaaagtg aactagaata tccgggtcag
    44341 attaaagtga acgttatcag agaaacccgg gcaattgaat atgcaaaata gtattaaaaa
    44401 aagcgtggaa acacgctttt ttaatattta acactatttt cctcgcacct tttgatgaga
    44461 tgttgcgtct ttatattgca gtgcaggctt gcttaattac cagctgcttg ctgcaatatg
    44521 cttataggga aatggaatgg aggatatata ttgaaaatat tgtttgttgg agatgtggtg
    44581 ggaaatccgg gaagaaaaac agtaaagatg attttgccgg agatgaaaag aaatttatca
    44641 atagattact gcatagcgaa ttgtgaaaat gcagcaggag gaagcggtat tacttatgca
    44701 gtagcgcagg agctgtttaa ttcaggcgta gatgtaatta ctttaggcaa tcatacctgg
    44761 tcaaagaaag aaattgtaaa ttttatagat acagaaacaa gggtagtcag acctgccaat
    44821 tacccacagg aactgcctgg aaaaggagct actgtaatag gcaatgataa taaaggatca
    44881 ataggagtat tgaatttatt agggagagtg tatcttgaca gtgtagattg tcctttcaga
    44941 acagcagaaa aggaaattca gtacttgaaa aattttacga aagtaatagt ggtagatatg
    45001 catgctgaag ctacatcgga aaaaactgca cttgcatggt atttggatgg taaagtaagt
    45061 tgtgttctgg gaactcatac ccatgtgcaa accgcagatg aaagaatcct tccatgcggt
    45121 acggggtata ttaccgatgc aggtatgacg gggccgtatg aagggatatt gggagttgac
    45181 agggaaataa tagtaaataa gtttttaaca tatatgcctg ctaaatttga actggcaaaa
    45241 ggaagcacac aatttaacgc cgtcctgctt gacattgatg aaaaaaatgg ccgcacatat
    45301 agaattaaaa gagtactgca agttatgaat gtataaaatt atattatttt cctaataaaa
    45361 aaaggattat tatgttttat gtagaattaa taagtgtaaa tatctacttt taaccgaact
    45421 accactattt gctttaaggg ggtaatatta aatggatgtt ttaaaagttt cagcaaaatc
    45481 caatccaaat tctgtagcag gagctttagc aggtgtaata cgggaaaaag gctcggcgga
    45541 gatgcaagct ataggtgcag gagcaattaa tcaggcaatt aaggccatag ctattgcccg
    45601 gggttttgta gcgccatccg ggatagaatt gatttgcatt cctgcatttg ccgatatagt
    45661 aatagacggc gaggaaagaa cagctattaa acttataatt gaacccagga aatagatgct
    45721 tcaattgatg catctaaaaa gactttaatg aagcgtcctt aaattaaaga gtgagtgata
    45781 ttagaaggag tgagtatttt tagcaggaca gtgagtaacc caaataacag tatatatgaa
    45841 aatatataca tgaaaaaaga tgaacttgag tatatgcagt tttcgcctct tactaaatac
    45901 aaccagatat tgacacactg ctttacaacc agaagaggtg gtgtaagtat tggaggatgt
    45961 tcaaccttaa atcttggatt cggacgtaac gacagtagaa caaatgttat agaaaatttt
    46021 aagagagtat gcgatacctt gaatattgaa ttggatgatc tggttctgtc ggaccagata
    46081 catggcacag aaattaaggt tgtagatgag aaagatagag gaaagggcat tgtactggat
    46141 agcgatatta aaggttatga tgggttggta actaatatag aaaaagttgc attggttact
    46201 ttctatgcag attgtgttcc catatacttt tttgatcctg tgaaaaaagt tatcggactt
    46261 gcacattccg gttggagggg tactctttat caaattgctg caaatatgat aagcataatg
    46321 gacaaagaat acaactgcaa tcctgaggag gtaatatcag ttgtagggcc atccataggt
    46381 cagtgctgtt ttgaaatagg aattgaagta ataacagagt tcgtgaagaa aatcatctgg
    46441 agtaaagatt tctgtaaaaa tataggacaa ggtaaagctt atattgattt gcagggaata
    46501 atcaaacaga cattaataca ttccggagta agaagtgaca acatatttat tagcggtatt
    46561 tgtacaaaat gcaataaaga tgtgtttttt tcccatagag gggataaagg taaaacagga
    46621 agtcttgcag caataatgca attaaggaga taaaacagtg agtaaagcca gaggcatatt
    46681 tttatcaatg gttattattg ttattttgct tattagtatg tcttcatgcc aaaaggaaga
    46741 ttacatttta aaaaatgaaa agcaaaattc agtattgcca tctttaaagg aagatggtgc
    46801 tgttgttgat gggggacctg tcaaaggagg caccctgaaa ttattcagta catttcccga
    46861 tactcttgat cccataactt caaagaaccc ctggcttcaa aattatttta gcctggtttt
    46921 tgaaagtatg atcaaactca ataagaacca gaagcctata ggaaatttag ctgataaatg
    46981 gtatacatcg gaagatggaa atacctggac tttccacata cgggatggtg ttgtatggca
    47041 ggatggttat cccttaacag cgcaggatgt tgagtttacc ataaagacta ttttagagcg
    47101 gaatcagaat acaatttaca ggtttaatct tgataatatt ttatctttta ccgctataga
    47161 ccgtaataca attgaaattg ttttaaaaaa gccaaatgca tttactgcag agttaatgac
    47221 tttccccata atatcaaagc gtttttacag caaaatagat aataatgata aggaagtatt
    47281 tgcaactaaa tttcctcctg gaacaggccc ttatagtata caatcctatg atgaaaataa
    47341 atgcattaag ttgactgcaa gcagatcatg gtggaagaaa aaaattaatg aaaataatga
    47401 aatgccttat atacccgatt tggaaatcaa gctttacaag aataccagag aggcattttc
    47461 tgcttttcaa atgggaagtg ttgatgtaat taatgttgac aataatagca ttggtattta
    47521 ttctggaaaa tcggatgtga ttacaaaaaa atatgcaaac ggggaattcg attttctggc
    47581 tttcaacctt tcaaataata ctttaagtga taagtatgta cggcaggcca tagcgtcatc
    47641 ccttaacaag agcgaaattg taggagcagt attaccggga tgtgcggtag aggccggcat
    47701 gcctttaata ccaaatacat ggttatatga aatggatgaa tgggttaagt cattttcaaa
    47761 taattctcag aaaacgaaac aatctgataa tgtgagagaa ttattaattg aaaatggctg
    47821 ggaggaatat gatggcaaat tatataaacg tatatatggg gcattgaggc ctttaaaatt
    47881 caacattatt gttaatgaag ataataattg cagagtaaaa gttgcggaga aaattgcaga
    47941 gatactaaac aacacaggta tggatgtcag tgttcataaa gaaaaatgga atcaagtgtt
    48001 gaaattggta aattcaaaaa tgtatgatgc agcaatattg ggttgcacag tacccccata
    48061 tcctgatgtt tcatatttat actctgctcc ttacttaaca taccagggtt acgcagcatc
    48121 ttcagctgcc actgcagcta caaatgtatc cggatttgaa aatgaagaag tatcgaaaat
    48181 tattgaaaag gttttttcct caaacaatcc agatactata aaaggattgt attcaaattt
    48241 gaaaaatgtt atcaatgatg aagttccata tataggtttg tatttttact atgatgctgt
    48301 tttttataat aaaaatgtga ggggcaatct tgacccttat ttatgggatg agtataataa
    48361 tataactgaa tggtatattt ctgagtaaaa taatactact atgtcaattc ggaaagcgat
    48421 gtattcctta cgggtcagca aaaattaccc tccagaaatg cgctttccat tggcaacata
    48481 acaaagtaga attaaatatg atcacgggag agaataaaca tgggaaacat actttggatt
    48541 gctataataa tattaataac tgctgccgat caagtttcca agtatattgt tattaaaagc
    48601 gttgaaacag gacagcttat acctgttata aataatttct tttatataac atatcatgaa
    48661 aatgcaggtg ctgcatgggg catattgcag aacaagaggt atatttttat atttttgact
    48721 atcgtaatat caattgtaat gatatatgta ttattcaaga caaaggacaa gatgctcaaa
    48781 ctagccttat cttttgttct tggaggtgct atgggtaact taatcgacag gattataaaa
    48841 ggtagtgttg cagattttct agatttttat tttgggaaat acaatttccc cacttttaat
    48901 gtagctgatt cctttgtagt aataggtact attattttag ctgtttattt attgttttac
    48961 tatgaggaaa cggaaataat aaagttatag ctaggaatcc gcttttatgg ttcagacttt
    49021 ccgagcaggc ttcctgggaa tagttaaaaa ggaaatagct gaaaatgaaa attttaaaat
    49081 tagtttctga tacaaacaat gagaggctgg atgcatggct ggccaccaaa cttaaggaat
    49141 attcgcgctc atacctgcaa aagcttatta gtgagggttt tatttttatt ggcaataata
    49201 atgtaaaacc aaactacaag cttaaaatag gcgaggaaat cctcataaaa atacctgagg
    49261 ctgagaagct tgccgtagag cctgagaata tagctcttga tattatttat gaagatgatt
    49321 gcatcctggt tataaataaa cctaagggga tggttgtaca ccctgctgca ggcaattatt
    49381 cgggaactct ggttaatgca gttttaaact actgtggaga caagctttcg gatataaacg
    49441 gtattatcag accaggtatt gtccacagac tggacaaaga cacttccggc atattagtta
    49501 ttgctaaaaa taatgaggct cataaattgc tttctcaaaa gcttaaagcc cgtgatgtga
    49561 aaagggtata tgccgcattg gttaaaggta taataaaaga aaacagcggt agaatccagc
    49621 tgccaatagg aagacatcca gttgatagaa agaaaatggc tgttaatccc aaaaacggaa
    49681 gagaggcaat aacaaatttt aaagtacttg agaggtttgg agattcaaca tatgttgaaa
    49741 taagtcttga aaccgggagg actcatcaaa ttcgggttca tatggcctat atcggtcatc
    49801 cggttattgg agatgaagtt tacggaggaa agcagaaaga atatggtcaa aataacatga
    49861 aaggccaggt tctacatgca aagctgatag gatttaatca tccggctaca ggtgagtatg
    49921 tggagtatga agcatcatta cctgaatatt tcaaaagtct cctggcagaa aaaagctcga
    49981 ataatgtcaa aatataaaat agctatagac aagaatttct aaatgtgata ttataaatat
    50041 aattaatttt aatttaatat agacctttta aataagtcct gtgaggctgc aaaggtatac
    50101 aaataacaag gtatgacaaa taccggacaa aactactaaa acgtgcttct tgcattcttg
    50161 cagaagcttt tttaatgtaa aataataggg tttcatcgcg agttatcccg atatcaggag
    50221 tgtgaaaaag atgttagaaa aagctcaaat aatggatgag gttgaaatta caagggcaat
    50281 ttccagaata gcacatgaga taattgaaag aaataaaggt gtagataata ttgtactaat
    50341 aggtatacaa agacgaggtg taccgcttgc gcaaagaatc gcccgtaaaa ttactgaggt
    50401 tgaaggcaag tcggttcctg tgggcatatt ggatataact ttttacaggg atgatctgag
    50461 catgttggct gaacatcctg ttattaatgc tactgaactc aattttattg ttacaggtaa
    50521 aaaaatcatt cttgtagatg atgtccttta tacaggaaga actgtaagag cagctataga
    50581 agcaattatg gatcttggaa gacctgaaat gatacaattg gctgctttga ttgacagggg
    50641 acatagagaa ttacctataa gagctgacta tattggaaaa aatgtgccta cttccagaaa
    50701 tgaagtagtt catgtaaaat tggttgaaat agacaatgtt aacaaagttg ttataacaca
    50761 aagaggaaat gactaaagat tttaaatagt aaggagtata tccttactat ttttttgaga
    50821 tatattgata ccattaaaaa actcagaaaa aaggatggga gataatgaat cttaaatcta
    50881 aagatttgct gggactgaaa gacctcacag cagaggaaa
//
LOCUS       DAOSDD010000003        50311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_408_length_50311_cov_4.875756, whole genome shotgun sequence.
ACCESSION   DAOSDD010000003 DAOSDD010000000
VERSION     DAOSDD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 50311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50311
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_50311_cov_4.875756"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..7452
                     /locus_tag="PK733_00445"
     CDS             46..7452
                     /locus_tag="PK733_00445"
                     /inference="COORDINATES: protein motif:HMM:NF040203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ09046.1"
                     /translation="MQNADLINFGAEFNDYWRDQIGLSYDGGQSFIPQNSISWNSATK
                     SIEATFGAPENNNGNAFHIAVEVYRSYGEMASWRGVFGAFKFIEISAETADFVPIESI
                     TVTGIPASGIVCDSPYPLNVDISPKNVTFPGYTWESSNPDIARVTGGNLVFYAEGKVT
                     ITLRSGEGEYRAEQGLPANDDVLVKTFTFFAGTPRLITGSISVGKSDTTTAVTARFND
                     NFDSYEDEWKTPVLTYEIRKADGTIVKSGNATRENDVTEVSLAFDDVTPKTVSPYESG
                     EFKPAYTITLKATAGTSSVSATANVYITPPPVTMECLTETSNALVGEPATFNFRIKNL
                     LPGYSSRYEITGGGGQEEGGLTNRSEESDPATELITLTGSVVFTPAVQGDVSEFRTIT
                     VYANNKDEKQQSISKRINIVNPSAALMSVKFYDGFDGTVINPEPGDKPDAFYGIRESR
                     IAALTEGSTYNSEEVFKYLDRMMHTKTLRVAVPEEWGRMKAEGASLPAEDRRDISLYL
                     SDVDKGKEITLSWENVNTQKKFSFNEDDLSGKVYAFRLLNALDEKHVSISYTNWSDLS
                     VTKNVIPYNGYVIIYEPSGIKGEVWMSQEGAGYRFATFTQPRGISRGYRTSWYDLSPM
                     VVSPLATDHGYRSQHPALAFTANTATMSDNPIAKFNSVGNTVPSVVRYCAIDKDRKII
                     AGTNGVTNPSAGSGNFELPFKALYENPNSQLMVEYEYTTGKEIRTQLEYYYLDKLEEN
                     LRLKKANSYAIVVSSYIQRLSVIGKDGVIQNFPVAADRFITINPGDIIELQLATASRA
                     VKRAELKLLNHRRTVDANGNIGDWIFDYTKASPAVSIEEIDLSAFTPNKYTTLRFLPT
                     REQMTLGCISHIRLVTVFGDGTSDAHNVCWGQMADAETAANIKKITDILNGQEFFHEK
                     QMEFRLGDLNVKENTRNVKYEGSLFTFNEEVAAEIDKIKINLASPSKNPFTYEVKRKG
                     DEYTIRGYANTDNMNKAGVVHLADRWKDATFDELFGETKRYVLQSSSKYRPNFPGIKG
                     YVEGKAIITPSGDIRFTFHEGRTYVESDFFHKPKKLFDLSYYCSWTAVFEGMVTSIFE
                     IKAPDNGGDPTVPFNFNLEESSWVFMNTRTAHNGISIDIGVYAVKVSLEGEIEDADYS
                     RKSIHRPYATDSQKIQWNARFSTGGNLYERNYNRIITSPTDVDIESSNMKKWYSWFRK
                     PGEEPFYIMHNIEATSSGWGSSKVSSFGMPSDFTGTDAYSQIMMVASADAFRLAARDG
                     DSHRLDVDELSAVRYYNGGKGIFYSDSSSNIVTSGIDGNATETVDAKAFGIDIVSAEN
                     GSAVASWGSYKGNLDTDALDNVGKDGILKYASGKTEIKAGIFNGTNWSITTLTDNEMA
                     DITPKTAANGTEGVVVWTQGILNDYHLEGKLPVITFSESRLMYARYNGSAWGKPAVLY
                     APSGESIADYSVAMTDDGNALVTVVMGSGKIAIVRISKSGEVTVINNSLPTTTKASLV
                     YNGKSYMLACSSDVKDAEGEDNGHIALTLYEISTDGFVTNTTFSGIPTDISGDFNLFR
                     NWSESGAAVLVWRGSGKDNSEARITIYASKVSEANEDNTVLVSAPIAAANINLPTSTG
                     PDGEGGAFTASYDAYIKGNDLKILTIFGESSSEESKSGESTPGESDRGVYLAETQAQF
                     KNTIEIASCVNDISYLMPGLQSNFNIKVKNKGFVPITSINVKIGSGESGTNTVCVLPN
                     DETAVTAAFIPTRDLPDSIDYTVTAVFSDGSESTVTVSVSLLQADIAAEILLFTQENG
                     DYNIKALISNNTPFSLSGKTVIAGIYQDPFGTVPVTEEKIDGSKFENETLGTSLLVDF
                     SFADAKDLPQSLYLIAKVYDSSGNEIPDNDSMNNILTVTNIVLVSSSSPGDDDDGSSS
                     NPGDDDDSSSSKPGDNDDGSSSSGGSSSGSTTTTITKPAKTPDQPVTAVAPVTATAGA
                     NGAASAAIPDKAITDAIAKAQADAKVQGKTASGISVGLNVNMPKGATALTASLTRSSL
                     DSLVNAGVSQLELNGAPVSLSLDLKALQEIQKQSSGSISITIAPATGLSKEAKALLGN
                     RPVYNITISYVKDGKTANVTSLGSGTATLSIPYTPGKNEAVGYLFGVYVDAKGKAQRI
                     PGSAYDVSSGSLLIPTGHFSVYGVGYTAPSAKFTDIGNHWGKEAIDYVVGRGLLYGTS
                     ETTFAPNIAMTRGMLVTTLGRLAGVDAKTYTTNSFIDVKADSAFRPYIEWAYKKGVVQ
                     GIGNQRFAPDRAITREEIAVIFANYAKATGYKLPVTRKTTAYADDSSIGSVYKTAVKA
                     MQQAGIMMGGSGNRFNPKSNATRAEVSSMLNRYIKLTIDSATAQGWALNYAGQWLYYK
                     DGKPLTGTQTIDGVKYFFETTGVLKTGWVKDGDNWLYYSRNKAAIGWLDISDKCYYFT
                     KDGLMVSGKWLQIDGKWYYFYADGALAKSTKVDGYEVDENGVRKSK"
     gene            8463..10766
                     /locus_tag="PK733_00450"
     CDS             8463..10766
                     /locus_tag="PK733_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HOJ09047.1"
                     /translation="MDIIMKILIPKCCEEVYCIAAEEFKSFYEKVTGTVLPIIREPQD
                     DDMIVIGSDAVNHYTAELMLSNKINEFKIRYCTDDYHIKTETVNGKKLLFLAGGRGRA
                     VMYAVYRFFEVCAGCRYFWDGDIIANVREININDIDIAESPHFEYRGLRYFAHRSLHR
                     FQAEHWSFEDWKKEIDWMLKKRLNMFMLRIGMDDLFQIAFPDIVDYPSNDEKLPEAGE
                     GHHDRTTFWSLEYRGKLRKQILEYAFKRDLMHPEDCGTMTHWYSMTPLQFLEKKKPSL
                     LSRDNKYEGVVTGKIWDVSCDENLNNYFKLTETHIQYYGKPEIFHTIGLAERIFSKDA
                     ENNLRLKLYTYRRITRLLQERYPNAKLFLASWDLWGQYRDVDEVQRLINNLNPEQSII
                     LDYTSDTLRSNNFTNWNVVGKYPYIFGMFHAYEPNSDIRGNYDITNKRLAIASKDIFC
                     KGMVFWPELSHSDTFALEYFADNAWNPCTLSVNERINKYCNDRYEMFSSKMIEIWSNF
                     MPIASIMHWCINLRSYMPREWFFDIFERGKYMFDSKSNVFEEFIKIRKKTEKFKMNAI
                     YALELAAEIMKDDTNNEFLNRDVYDIARTICGRYLQCQLINTAEVFLKCHSTSGTEGN
                     YRDLLESKYRSCIKLMEILIDILSGHEDYSLFESFKKVKSVESVNPVFETTLKNNATG
                     NYCRSYIYENAKYLYMPEMKLFFEWLDKIREENSIKELPYFELYYEKAKENIQIFFKT
                     PLADMIPKYKKNISDILINLSQELKVI"
     gene            10815..11825
                     /locus_tag="PK733_00455"
     CDS             10815..11825
                     /locus_tag="PK733_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09048.1"
                     /translation="MIKMQTSRKTGQKVSQKIIQKTDNQIDQSMISRRNHSLLIKGIF
                     KNYELYLLSLSGLIYLIVFKYVPMYGITIAFKDFNIYKGIFQSPWVGFDHFIRLFTSA
                     KFLQVFTNTLLISIYKLIFTFPAPIILALLLNEIRQLVFKKAIQTISYIPHFISWVVV
                     SGMFLDILSPNTGILNKLIKSMDFEPIFFMGDKAYFRSVLVVTQIWKEVGWGAIVYLA
                     AIAGINPELYEAAIVDGASKLRQVWHITLNGIKPTIAILLLLRIGNLLNAGHEQILTM
                     YNPAVYEVADVLSTYVFRVGLGQMEYSFTTAVDLFNNIIGCVLLFSTNHLVRRLGEQG
                     IW"
     gene            11879..12745
                     /locus_tag="PK733_00460"
     CDS             11879..12745
                     /locus_tag="PK733_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09049.1"
                     /translation="MLIRSRGEKTFEVFNIFIMILLGMVTLFPLIHIASKSLSDEAYV
                     LARDIVLFPKGLQFEAYRNIIGNARFARSFQNTIILTFVGTSINVMMTVITAYPLSRK
                     RFPGQSAIMVFYVFTMFFSGGIIPSYLLMRDLKLLDSMWSLILPGTINVYNMILIKNF
                     FMSVPDSLEESAKIDGCKQIRILFSIFFPLSLPAISTITLFYAVGHWNSYFAALMYLN
                     SRNKYPLQLFLREMLIEETVDVVDISNLLDVAPDSVKAATIIVATVPILVIYPFLQKY
                     FVKGVMIGAIKE"
     gene            12911..14398
                     /locus_tag="PK733_00465"
     CDS             12911..14398
                     /locus_tag="PK733_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09050.1"
                     /translation="MKRILSIIVALMIVILTFSGCVTKDAGKAEQKTGNTEATQKTEE
                     RPKIKMLVDYNIGVANFKADENENKNIIYDWIIENSGFDITFEVKPKDNPDQKVSIIM
                     ASGNVPDILEISNKTMYSNFASQGALTDVTELVKTAPNIQSLIPKDSFKAIEIASKIY
                     AVPRPPENEGYGQGIYARLDILKNNGLQNPKTTDEYYNVLKSVTDNEKGVWGITSDGP
                     FIGGFMGAFGIAAKMKEKDGKVVYTYTQPEFKNYLTYIKRLYDEKLLDREFSVNKGSA
                     KEKLISEEAVMAFSDFSVSKLVFENLKSKNSSVELGYLDLPIGPDGSQGVPKSRPVIR
                     WFAIPIQSKNAEAAMKYMDWVCREDVQKVLALGFEGVHYNMVNDIPVPTEKMPDIVWR
                     VIYQRLENGTFPMRIQTKGFWPFYEPTLKNPLVENIAEFVPAIEAVDKKSNELQDYVV
                     ENSIKFITGERPLSEFDAFVQEYNKLGAEESMKLINEWYAEFSKK"
     gene            14803..16425
                     /locus_tag="PK733_00470"
     CDS             14803..16425
                     /locus_tag="PK733_00470"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09051.1"
                     /translation="MLNVLIVDDEKATRDGLAQCIDWEMYGMTVIGEAAEGKEALKIM
                     DRIPVDIVVSDVVMPVMDGIQLVAAINKNKPDTKVILVSGYSDIPYLKSAISLGAVDY
                     IFKPVKLKELKAVLSRISSHFEKQMEQKKIFNEMKNKLEQSIPYLRERFITKLISGKI
                     QDDTNILEQISFLGMNLNLNGYFSVMTVSMESEHEAGRKLAGDNELYKMVGLSALQEY
                     FDASTRIWSMSIDEVNFVLLLSTDYTIDYNMSLKIASEIQQIINQYTGYSISIGIGSE
                     VARLTKAYISYQQAIRALEQKFFLGEGQIIHFNDIQNVTFSKGFYPEGIVDEIIDMVR
                     LGNKERVEILVDRMFEDIFQNSCLNLKLVQNICFEAVTLANRQLLQARAIDESHEAGL
                     LDMSTLKDIFKPKSLKETHIWLKNLLYDAAERICTVQSNISKNIIIEFIKKAINDKYH
                     EDISLLTMALEFCLTPNYLCLIFKKETGETFNDYLTKVRVQNARELLKDPTYKIYEIA
                     EMVGYRDSDYFARVFKKITGFTPSEFRKGLIL"
     gene            16422..18233
                     /locus_tag="PK733_00475"
     CDS             16422..18233
                     /locus_tag="PK733_00475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ09052.1"
                     /translation="MKKLSKLTRSTRLRDRFTLIYIFFLAIPMLFVCLYLYGQLSIIS
                     EYQGQQVVERAIRQSGASIASLVEEIENVAGQVFINTTLVKYITDEDSSDANQYSELK
                     YIENIIDQILYYPQVGYVRMYIPSYKLYARNYAIMFPIEDIMDTENYKQLIGRERQIG
                     WTDSYSYSIKDQGSRDVITYMVALKDFTQIARIVGCVCIDIKQSTFIEKLKVTIIGDN
                     AVSLLLSDRGDVMASASGDYCKLDTNLIDEIILSYRGKDNVDFHELNGKYLTVKVDGK
                     KYVAISHTIKSNGWTIVYTMPHSDILARSKKLMNTIIITVMFIITISVMSVVIVTDRI
                     TKGISRVISKMNDIDIVYHNVSPHMQIDNVSYNDEIGSLQKTFNGMISRIYQLNNEIY
                     QTKILKREAELRALQAQINPHFLYNMLDSINWMALKAGNDAISDVVSDLGRFYRISLS
                     GGKDIITIAEELEHVKLYIALQSKRFEDAITVNYNVDSTVMGLKMAKLTLQPLVENAI
                     IHGIMEKPEKKGQIFINAYIESDIVKIVVTDDGVGFGLKNQNDVSINNHHGSGYGLKN
                     VDQRIKLYFGDNYGVRLYRSSDMLTIAEISLPAIEMI"
     gene            complement(18286..18657)
                     /locus_tag="PK733_00480"
     CDS             complement(18286..18657)
                     /locus_tag="PK733_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09053.1"
                     /translation="MNGEIYNACSIVAAAKRALRKSKNIDYTLGIYEKHIEFYFLPIG
                     LFMRKRQIAFSVNSWYKECLSRGLDVIKFMTSDRVKDRHLLGFAGATVNSTETLFKHL
                     DSVKGLPAKYLLFYSRHQRIQ"
     gene            19079..19267
                     /locus_tag="PK733_00485"
     CDS             19079..19267
                     /locus_tag="PK733_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09054.1"
                     /translation="MLGLIYLRFADSKYGKYEEEINKEYYELKGNRWKKIEGIYDNIC
                     RVRKERGIGLTVCTAIAT"
     gene            19420..20715
                     /locus_tag="PK733_00490"
     CDS             19420..20715
                     /locus_tag="PK733_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ09055.1"
                     /translation="MYRDAMNDLHKWKSKKNKKPLIIRGARQVGKTWLMREFGSSAYD
                     EMVYINFDNNTQMKELFSIDMRIERIITGLELYAGHKIDPTNTLFIFDEIQEVPQALA
                     ALKYFNETAPEYQIICAGSLLGVALHSGTSFPVGKVEFLELYPLSFNEYIRAMGKQQF
                     VELLEKQDFEMATTFKQEYIDLLKHYYFVGGMPEVVKTFSDNRDFNEAREIQQRILDA
                     YEQDFSKHAPNEIVPCIRMLWNSIPAQLTKENKKFIYGIIKEGARAKDYEMALMWLAD
                     CGLVHKVHRVKAPNIPLKAYEDLKAFKLFLVDVGLLSCMVRLNQRVLLDRNGLFKEFK
                     GALTEQYVLQQLKTIKGIETYYWTNDRGSAEIDFLIDNGTDVIPIEVKAETNLKAKSL
                     KTFCEKFEPGMAIRTSMSDYKQEDWLLNLPLWAVEMVNN"
     gene            20972..22306
                     /locus_tag="PK733_00495"
     CDS             20972..22306
                     /locus_tag="PK733_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOJ09056.1"
                     /translation="MEKLFKLRENGTNVKTEIIAGITTFMTMAYILIVNPNILGDAGM
                     ERGAVFTATAVSAAIATILMAFLANYPVALASGMGLNAFFAYYVVGVLGYSWEIALTA
                     IFIEGIIFVILTLFRFREAIINSIPANLKYGITVGIGLFITFIGFQNAKIIVNNDATL
                     VGLGDLKSLTVILSIIGILVTTLMLHKKVKGALLWGILVTYVLGIICQLTGLYVVDPA
                     SGMFSLIPEKIISLPPSVAPIFLKFDFKGALALGTNFFVVMFAFLFVDLFDTVGTLIG
                     VASKANLLDKEGKLPKAKEALMADAIGTVAGACLGTSTVTSYVESAAGVAEGGRTGLT
                     ALTTAALLIVSLIFSPILTIIPSFATAPALIIVGLFMVSAISKIDFTEDFTEALPAFL
                     AMIVMPLSYSIADGIMAGIISWFVLKLISGNIKKIHPVIYVLVALFILKIVA"
     gene            22629..23144
                     /gene="purE"
                     /locus_tag="PK733_00500"
     CDS             22629..23144
                     /gene="purE"
                     /locus_tag="PK733_00500"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOJ09057.1"
                     /translation="MNNGRLKVAVIMGSDSDFPTLKGCIKILKDFGVEYKVMVCSAHR
                     TPEKAAEIAKNAESEGFGVIIAAAGKAAHLPGVLAAYTPIPVIGVPIKASTLDGLDAL
                     LSIVQMPTGIPVATVAIDGADNAALLAIQILSVADNELRGKMKNYKEKLASKVDEKNL
                     ALQEILSMEGL"
     gene            23290..24672
                     /gene="purF"
                     /locus_tag="PK733_00505"
     CDS             23290..24672
                     /gene="purF"
                     /locus_tag="PK733_00505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ09058.1"
                     /translation="MIYDINDKIREECGVFGILDNDGHNVAQLTYYGLFALQHRGQES
                     TGIAVNDSGTIIYYKDIGLVPEVFNSTILDHLKGQAAIGHVLYSPDSSNRENVQPIVL
                     KYKSGQMAISYNGNLLNASKLRQDMEERGAIFQTNSDAELIASLISRYRIISDNIEEA
                     LIKVMKDIKGSYAFVIMTPKKLIAIRDPLGMKPLCIGKLDNSYVVASESSAVDAVGGK
                     LIRNVNPGEIVVIQEDGIKSIQTEVTGKTKLCIFEFVYFARQDSYIDGASVYRARLEA
                     GRRLAIEHPVDADLVIGVPDSGLIAALGYSRESSIPYGSGLIKNRYVGRTFIKPGQSQ
                     REEAVRIKLNVIRNEIEGKRIVMVDDSIVRGTTTKAIVHMLKEGGAREVHIRISSAPY
                     KFPCHFGIDTPSAKHLVASNYSLEEIRKMVGADSLKYLSLDGLLKTPIGSDCDFCCGC
                     FTGDYPLKVE"
     gene            24886..25908
                     /gene="purM"
                     /locus_tag="PK733_00510"
     CDS             24886..25908
                     /gene="purM"
                     /locus_tag="PK733_00510"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOJ09059.1"
                     /translation="MTTYKDAGVDVEAGYEAVRLMRDHVKRTFRPEVLTDIGGFGGLF
                     SLNKEKYNQPVLVSGTDGVGTKLKIAFLMDKHDTVGIDCVAMCVNDIVCSGAEPLFFL
                     DYIAVGKNRPERVAEIVKGVSEGCIMSRCALVGGETAEMPGFYSEEEYDIAGFAVGIV
                     DRDKIIDGKDIQEGDELIGLASSGIHSNGYSLVRKLLNPTPQKLHEYVELLGGTLGDE
                     LLKPTKIYVKTVLDLIGRFKIKGISHITGGGFIENIPRMIPEGLRAEINIGSWLVLPI
                     FNLLKNSGNIDEKDIYNTFNMGIGMVMTVEKGTAREIIDYLNRDGEQAYIIGKIVRGE
                     AGVELC"
     gene            25902..26537
                     /gene="purN"
                     /locus_tag="PK733_00515"
     CDS             25902..26537
                     /gene="purN"
                     /locus_tag="PK733_00515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOJ09060.1"
                     /translation="MLKIGVLVSGGGTNLQAIIDNIESGDLPGCSIALVVSTRADAYA
                     VRRAEKHNIPAVCISRKNFESIDQYDEALINQFEKYNVELIVMAGFLSIVGEKFVNKY
                     SGRIINVHPSLIPAFCGKGYYGLKPHEKALEYGVKVTGATVHFVELEPDAGPIILQKA
                     VYINDDDTPEALQKRVMQEAEWEILPDAIKLFAEGRLVIEGRRVKVKKGGF"
     gene            26540..28177
                     /gene="purH"
                     /locus_tag="PK733_00520"
     CDS             26540..28177
                     /gene="purH"
                     /locus_tag="PK733_00520"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOJ09061.1"
                     /translation="MIKRALISVSDKTGIIEFAKGLEKLGVEIISTGGTAKALRDAEV
                     KVTNVSDVTGFPECLDGRVKTLHPKIHGGLLAIRDNQEHMKQLKDLGIEPIDMVVINL
                     YPFKKTIEKEGVSLEEAIENIDIGGPTMLRAAAKNYRDVAVITDPADYDKVLAEISKS
                     GEVSPETKFLLAYKVFEHTCHYDTLIAHYFRGQMLKRNQAIAATHDISNSNLKAGSND
                     SQEKGIYNIEEFSQTLTLTFEKIQDMRYGENPHQKAAFYKEPGTNAGCLTNLHQLHGK
                     ELSFNNINDTNGALDLLKEFEQPTVVAVKHANPCGVASADSIYEAYLKTYEADPVSIF
                     GGIIAANREIDAKTAEEISKIFVEIVIAPSYSEEAFNILSRKKNIRILTLDDISKKLS
                     KEAYELKKVSGGLLVQERDTELINIDEISYVTNKRPGAKEMEDLIFAMKIVKHTKSNA
                     IVLVKDKQTVGIGPGQANRVTSVRIALNYAQEKAKGAVLASDAFFPFSDWVDESVSAG
                     ITAVIQPGGSLRDQESIDACNEKGIAMIFTGMRHFKH"
     gene            28394..29653
                     /gene="purD"
                     /locus_tag="PK733_00525"
     CDS             28394..29653
                     /gene="purD"
                     /locus_tag="PK733_00525"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOJ09062.1"
                     /translation="MKVLVVGGGGREHALVWKAAQNPSVEKIYCAPGNGGIAKIAQCV
                     PIKATDIKGMVEFSKSNNIDLVIVSPDDPLAAGMVDALQEAGIRAFGPLKNAAVIEAS
                     KSFSKNLMKKYNIPTAGYDVFDNCDKAIAYLKEYKYPVVVKADGLAAGKGVIITETYE
                     IAVEAVKNIMDDKIFGEAGNKVIVEEYLTGHEVSVLAFTDGKTVIPMVSSQDHKKVFD
                     NDLGLNTGGMGAVSPSRIYTPEMADYCMKKIIRPTVEAMNKEGRTFKGVLYYGLMITE
                     SGPKVLEYNARFGDPETQVVLPRLKTDIIDIFNAIIDEKLDEINIEWDERAAACVIMA
                     SGGYPGKYETGYEIKGLCDAQKDKDTIVFHAGTKYENDRYYTSGGRVLGVTALGETLE
                     IAVKKAYEGVSKIEFENMHYRKDIGRK"
     gene            29788..30405
                     /gene="nadD"
                     /locus_tag="PK733_00530"
     CDS             29788..30405
                     /gene="nadD"
                     /locus_tag="PK733_00530"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOJ09063.1"
                     /translation="MKGKFKRIGISGGTFDPIHYGHLIIAEEIRETMELEKMVFIPSA
                     NPPHKVGIGVTNATHRYNMVKMATESNPWFAVSSIEMERGGYSYTVDTLVQLKQIYGE
                     DTVLFFMTGADVIPELVTWKNFEKLFSLCEFVAVLRPGFNRESLIKEIEFLKSHFMAK
                     IHIVDAPLIGISSTIIREKVRNGKSIKYLVPENVEEYITENGLYK"
     gene            30519..31097
                     /gene="yqeK"
                     /locus_tag="PK733_00535"
     CDS             30519..31097
                     /gene="yqeK"
                     /locus_tag="PK733_00535"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOJ09064.1"
                     /translation="MVLDQIKDKIKLNIKPSRYIHSENVMEAAKNLAGIYGANVDKAA
                     IAGILHDCAKGRTAKELLSLCEKFDMHVDNIEKRQPELLHGPVGAFIAEMDYGIKDEE
                     ILNAIRVHTTGKEDMNILEKIVFLADYIEPGRNFPGVCEIREWACKDIDRAIIIALDT
                     TIKYVIAMGGLIHPDSIGARNYLLLQRANIQN"
     gene            31332..31670
                     /gene="rsfS"
                     /locus_tag="PK733_00540"
     CDS             31332..31670
                     /gene="rsfS"
                     /locus_tag="PK733_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOJ09065.1"
                     /translation="MDSSVLAEKITEILLDKKAKDVDVIKVENITILADYFVICTGTS
                     TTHIKTLADEVIFKLKEEGIEFHHMEGYESSRWILLDYGSVVIHIFHEEERKYYSLER
                     LWSDGIITRK"
     gene            31880..34396
                     /gene="leuS"
                     /locus_tag="PK733_00545"
     CDS             31880..34396
                     /gene="leuS"
                     /locus_tag="PK733_00545"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOJ09066.1"
                     /translation="MKSYNAKDIEKKWQKKWEEENTFKVEENGKPKYYVLEMFPYPSG
                     NLHMGHVRNYSIGDVVARFIRMNGYNVLHPMGWDAFGRPAENAAIMHGTHPFDWTRSN
                     IEIMRGQLKELGLSYDWDREVTTCHPNYYKWTQWMFLQMYKNGLAYKKKSNVNWCPSC
                     ATVLANEQVVNGKCERCKADVGKKDLEQWFFRITKYAQRLLDDINKLKGWPDKVKLMQ
                     QNWIGRSEGVEVDFKVDGMDKGVRIYTTRPDTIFGVTYMVIAPEHRMTTEIIKGTPQE
                     QTCLEFITRMQHMNEITRTSEETEKEGVFTGRHVINPLNGDKVPLYIGNYVLAEYGTG
                     VVMAVPAHDDRDFQFAKKYNLPVKVVIQPEGEELSADTMKNAFTEVGYLVNSGIFNST
                     RSDEAIGKIIDYIEEKGYGERKVNFKLRDWLISRQRYWGAPIPVIYCDDCGIVPVPEE
                     DLPVILPTDVKFRGTGESPLISSQSFLKAKCPKCGKMGRREMDTMDTFVCSSWYYMRY
                     CDPCNERLPFSKESTDYWMPVDQYIGGVEHAILHLLYSRFFMKALHDMGFVSYDEPFT
                     NLLTQGMVLKDGAKMSKSIGNIVSPEEIIEKYGADTARLFILFAAPPEKDLEWSEQGV
                     EGCYRFINRVWRIVDEYSGSLKDDQACACSSSCGCGCGSSSGEKITGADRELRYIVNY
                     AIKKVTEDIRERFNFNTAISAVMELVNALYAYKEKVAENKRNPFVIKEAIDSLIVMLA
                     PFIPYVTEELWQSIGGEGSVHEQIWPSYDPEALVKDEVEIVVQINGRIKEKIMIPTGL
                     DKDNTEKEALNTEKVKQLIEDKAVVKVIVVPEKLVNIVVK"
     gene            complement(34538..35752)
                     /locus_tag="PK733_00550"
     CDS             complement(34538..35752)
                     /locus_tag="PK733_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ09067.1"
                     /translation="MYIDRHLEKVILKASINFPVVLVTGPRQVGKTTLLSKMAEENRK
                     YITLDNPIVRELAVSDPALFMQRYQPPVIIDEIQYAPQLLPYIKMYVDQHKNKGDFWL
                     TGSQVFHMMRNVSESLAGRVGVINMLGLSTSEIQGYQGNPYKTNIKDLLEKVNYVRPQ
                     NLREIFSRIFKGSMPALYETEQDTEMFYSSYVSTYLQRDIRDLTQVGDELAFLRFMTC
                     CAARTSQMVNYADMAKDVGISPPTAKQWLSILVSSGVITLVEPYFNNSLKRLIKSPNM
                     YFLDTGLCAYLTRWTSSETLEVSAMSGAFFETYVVSEIIKSYYNAGKRAPIFYYRDSD
                     NKEIDLIIEQDNTLYPIEIKKSGSPKKDAIKHFEVLQKTGKQIGKGNVICLCSDIIPI
                     DKDNYFVPAWLI"
     gene            complement(35842..37461)
                     /locus_tag="PK733_00555"
     CDS             complement(35842..37461)
                     /locus_tag="PK733_00555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ09068.1"
                     /translation="MGNIQSTKYKKSGYGTCWVFRKYLISNVSLSFLYIILVLSLIFT
                     AFSGIVYADNSSSLNSPIVPAADSVSYAANLELPELNIEAEGYILIDSRTGLVLYSEN
                     ADKTEIYPASTTKIMTAIIALENEDINKVMTASQAAIDDIGPGGMNIGIMPGEELTLW
                     DLLNAMLVCSANEAANIIAENTASSRNEFIDLMNKRAKELGAVNTHFINTNGMHHMDH
                     YTTARDMALIARHAMSLPAFREIVKKDSYTMPSTNKHSTWNTLYTTNKLLRYGTKTNC
                     NFNIIGIKTGFTTPAGHNLVSAAVNEEGMELISVILGVKNERAPENIYKYTETLLKYG
                     FENYSLRTLIKQNSPAGNVSVKDAKDKAVLNLIAETDVSSVFPANLSYGTPAYAYAYV
                     GKDKDTDASNDVLAGEDKSANAMSNNWSVIKKVHVTPTISAPINEGEVLGYMEFWKGD
                     ILLGKTNLITSTSVEKSNKPKFIRAIQKLAGNIARAFSGKSGSIKNSALYKIAKSIFI
                     ILFSFGALRFSLRRISRIINIKRRMIKYRSK"
     gene            37609..38607
                     /locus_tag="PK733_00560"
     CDS             37609..38607
                     /locus_tag="PK733_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ09069.1"
                     /translation="MELRMFNKRLYIKKEYVSISIIFVIMVIILIIYISVKNNNEVVI
                     DSCKSPVRDSAKDSDNDPVNNNPVKDYPEANPKANPKADPKADPKADPKADPKADTKA
                     DPEADPKADPRAAPEADPKADPEADPKADPEAGETTLEIKVYIIGCVNNPGVITLKKG
                     QMIYEAIEIAGGATAEADLENINMVYILEENIMINILARDKDRKLITSDSEAGNNGIP
                     DINIAGSGIQIIRDTGDAVIKTGGKSTDNNMGQTDGTGKSSDSGNINNSKGKININKA
                     SVNELDTLPGVGKATAEDIIGYREKYGRFAKIEDIMKVPGIKEKKFEKIKDFITAD"
     gene            39414..42371
                     /locus_tag="PK733_00565"
     CDS             39414..42371
                     /locus_tag="PK733_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09070.1"
                     /translation="MKFPVKKAGNILMAFMLLIQLIMSPIKVLAEEPLRSKFNYGNEN
                     TSVEYYRKVSEPYYAALLKEYGEKSYKGANIPEILVEPADIVLPEGKELKLESKLGVS
                     DYPAFKWLCDIPWAEWNFEISSEGLYEIELEYYMLPGTGNPATRSLKMDGIIPFFEAS
                     NIVFYRGWKDESEPIVNSIGDEVRPSQVEIPGWRVMGLCDAQGYYSQPFRFYLKPGKH
                     TLRMEYVDQDMAIGRMRVRPVKELPAYEQVKKEYKNKGYANIDETVETVELQAESTAT
                     EKSDPTIRRERNGDPVCKPESSLYRKLNVLGGYRWRKGNQSITWEFDAPQDGLYKIAV
                     RALQSWNDGLPSYRQIDIDGEIPFKELEEYKFVSNRKWRTEVLDDEKGEPFLFYLTKG
                     THTISMTVKMGALTPIILSLNDDVILLSNLIRDITKVTGSNPDPNYDYDFFRTIPHLK
                     GNMEILMESLQSKYDMLEDISEKLPAMANNLMTIKSQLAGMVKDPFSIARRLNDLNNA
                     LSNLGNWYLSLQNQPLMIDYFLVGAPGVDWGNEQSNVFQKIKATLANFIVSFKKDYNN
                     IGSVVKDDVDIKNTINLWVSRGTEWAELIKEMVDEKFTPETGILVNINVFPAGQLHAG
                     SVNALMLAIASGRAPDGALGIDSGSPVEFAIRDAVYDLSKFKDFQEVSKRFLPSVFTP
                     FKYNDGIFALPETMNFIAMIYRKDIISEYGIRLPNTRDELYNNVLPMLYQNGLQFNYP
                     ADFSQFIFQHGAEYYLDNGARSGLETPESYRAFKEVTELYTHYGIPVSANFFNRMRTG
                     EMPIGIGDYNLYIQLSVAAPELAGRWGIAPIPGTLRSDGTIDRSNGSITGQAGVILNQ
                     SKKKEETWKFLKWWTSTSVQTKFARELEAIIGTEARWNTANLEAFTNLAWKKEDLEVI
                     KEQWQWAKGVPVVLGGYYTGRYLSNAWNSVVISGVPVRDALESAVEEINRELRMKREE
                     HGIFDNQTQGVGK"
     gene            42368..43390
                     /locus_tag="PK733_00570"
     CDS             42368..43390
                     /locus_tag="PK733_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ09071.1"
                     /translation="MRANTKVETKIKTEAEIGSAKIGVIEVNARAKMADGTGTKPKIK
                     PKIKPSWRKSLIGYVFMLPFLILFFIFIIIPVFVALGTSFTNFNMLQPAKWIGLTNYK
                     LLFLDDVIFLTAIKNTLIFACITGPIGYFMSFIAAWVINQLRMRNAFALAFYAPSITS
                     GIAMSVIWLYFFSNDRYGLFNNIFINLGFIKEPILWTLDAKYILPVIILISIWMSMGT
                     GFLVFLAGLQNIPREYYEAASIDGIKNKFQELWLISLPLLKPQLLFGAINSIVGSFGV
                     FDIAVSVAGMPSPNYAAHTIVAHLYDYAFIRFQMGYASSVAMVLFLMTFILGRVVMKM
                     LSSKGE"
     gene            43540..44472
                     /locus_tag="PK733_00575"
     CDS             43540..44472
                     /locus_tag="PK733_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09072.1"
                     /translation="MEVNVVKRFVINTYRKLQKRRAAKTAYTRRYEKTTAWDVLLHIF
                     MVALVAFTALPLVYMISTAFKPLDELFIFPPRYFVRKPTLQNFFDLAISLESSTVPFI
                     RYIYNSLVVTTITVTCTVIVSALGAYGLVKHKPIGHKVVFNFIIAALMFSPHVTQIPR
                     YMVVNKLGLLNSYWALIIPSIAVAYNFFLMKQFTEQFPDELLDSARVDGANEWTTFWK
                     IVMPSLTPAWSTLIVFSFVSNWNDYFSPLIYITDNAKKTLPPALQTISGGGSIGRAGA
                     VAAATMLMTLPTIIIFTTMQARVMETMTHSGIKG"
     gene            44487..46631
                     /locus_tag="PK733_00580"
     CDS             44487..46631
                     /locus_tag="PK733_00580"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ09073.1"
                     /translation="MNKFNRAISFFIVILIVFSLWTNIAQAASRDYIIDDGRRIPIPE
                     TYILKDIIYNVKNHGDEIKYYEAPTDLFINEQDYLFIADTGNNRIVKTNKSGEMIAVF
                     KEANGKAFNSPKGVFADAQGNLYIADTGNQRIVHLSDTGEFVEEFVRPKSDLLSETFT
                     FDPTKLCVSPTGYIYVLKGENILILDAYNNFRGYMGQTDIGFRLLDVLLRIFASEEQK
                     RIIRKRTAASYLNISMNDKGMIYAVTMDYATGEIKKLNSVGSNIYRKYKTLQKGANLL
                     NLDFLTTMKLESRSFVFGERNDGEGRVIMPLFKDIAVDKNGIVTVVEDVTGKIYQYDQ
                     EGNLLAAFGGKSDQQGRFSAPEAIEVDDNGCIYVLDRVLGNIQVFEPTNFIKMVHNAV
                     NLYAEGEYNGAYNQWQKVLGIHSNYPLAHLGLAKSLFKQKMWKESMEEYKQAEDRDGY
                     SKAFIQYRYEVVRNKFPIVLLVLIIIITIFVLLLRLIGKLAAEGIECFYMNGGKRFTI
                     IEQLKLSLGVILHPRETFELIKDKRDSMSIAPGVIILGVLFLVRIFYIFTVHFPLADI
                     DIRDSNIVLEAVKLLLPVVTWVIASFAITAILEGESKLKDIFIASSFSMVPYILVTFP
                     IAILSNVLARSELTLYAVIINGSWIWIFILFLIQAKTLNDYKFGKTISVSMISVLNMA
                     LIWAVVLMAYVLTGRLFQFVAGIFREIRMTFN"
     gene            46652..49060
                     /locus_tag="PK733_00585"
     CDS             46652..49060
                     /locus_tag="PK733_00585"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ09074.1"
                     /translation="MIKKTKSLLLIKKFKSAIKENKVNRNTYFKSKIYSIAALITAMI
                     VVSVFWGINVTAEPANSENTRHDNTGIEVVSQSKKDENSIHQTEINKSKLTYTTVPDG
                     YKIIEQNSKFALYCNTKTGAIIVENSETGYLWRTIVDYPEDYALENLSAHWKSYISSL
                     FTISYTDLQKNEGLMEKAFSSTDATITNSSIIKDGISLDYDFNEIGINITIDIILQED
                     GLIISIPSEKIKENKNFGLVNIELAPYLGTENDNLDGYIFYPDGCGAIMKYEKSKERP
                     KFIKPYKGFIFSPEKVDISEYITMERNQKYNVMLPVYGIRNGDDAFLAIASKGAEETA
                     ILVYPYGYSINLNRAGFEFYYRHFYNTILSNITLRGRDTAKNISGLKADKELIMQDHE
                     VTIVFLDKSDANYSGMANAYRDHLMKNNELKRVIKNTDSIPLEISLFMGIKEERMLFD
                     KYISMTSFQQAKMIADKFKDNGIDALQISLIGWSKGGYGIYPDIWPPERKLGGALEMK
                     KLLDYTSENSIQVFLRTNAIDAIADNKTFSKRNDVVIQGNGLPATGFNKDRFILSPFA
                     YFERIKKLINIIPVEDGTGLALEKTGKMLYHDYNKNNPSSRTQTMNTWGATLLLANNQ
                     NKPVGVEGGNVYALKYADYLFNIPVKSSGYHITDETIPFFQMVVHGMIPYTSEPGNLA
                     YDLTIQKLKWIEYGCMPFFELSYEDTTKLKYSRYNRLFNSQYEYWLNTAVDIYKEFND
                     RLQETWNTEMIKHEKLNANLVKISYSNGAVLYINYSDEDIIQDGYRIGAKDYIVIDRK
                     GAVR"
     gene            49057..49968
                     /locus_tag="PK733_00590"
     CDS             49057..49968
                     /locus_tag="PK733_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ09075.1"
                     /translation="MKKSFKITGEKRHRLEGLIFVSPFIIGTLIFFIFPLFVSIKLSF
                     GKLKKMEGFVIEWLGLDNYIRAFILDTSFIPMFLQVIRKTIVQVPLIIVFSILLAMLI
                     NKNIKFKGLFRTIFFIPFLLGMGDVMNQLLGLGIDKHVFSFVESNLLPREFLVYLGPK
                     VVEAVESFFGIIVVVLWSSGVQILLFLSGLQSIPVSLYESAKVDGATEWEMFWKITLP
                     MISPIMLLTIVYTIIASFTDISNPILAYIQSNAFRSLRFEYAAAIGWVYFLFIILLVS
                     LVFILLGNHIYTTSELKGGAKNERKDY"
     gene            49949..>50311
                     /locus_tag="PK733_00595"
     CDS             49949..>50311
                     /locus_tag="PK733_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09076.1"
                     /translation="MSEKIINNIKSKLVIKRTGNIKHNVFRGATYLLMLDMAFVFLFP
                     FLYMVVTSIKSNKDLYDFTVNWVPRTIEFKNYILAYKALEYPRFFKNSFIMTTVTTIG
                     HLLSCSFIGYGFARYNFSG"
BASE COUNT        16804 a         8351 c        10635 g        14521 t
ORIGIN      
        1 aaggagagct ataaaggact tgaaaacata caggtgtccg tgcctgtgca aaacgcggat
       61 cttataaact ttggcgccga atttaacgat tattggaggg atcaaatcgg cctgtcatat
      121 gacgggggac agagtttcat accgcaaaac tcgattagtt ggaattcggc tacaaagagc
      181 atagaggcca cattcggggc ccctgagaac aataacggca acgcttttca catcgccgtg
      241 gaggtctacc ggagttacgg tgaaatggca agttggaggg gagttttcgg cgcttttaaa
      301 ttcatcgaaa tatccgccga aacggcagac ttcgtcccaa tagagagtat tactgttacc
      361 ggcatacccg cttccggcat cgtatgtgac agtccctatc cgctcaatgt ggatattagc
      421 ccgaaaaacg tcacatttcc cggatatacc tgggagtcca gcaaccctga tatagctcga
      481 gtaactggcg gcaatcttgt attctacgca gagggcaaag tgaccatcac cctgagatct
      541 ggagaagggg aatatcgcgc ggagcaaggt ctgccagcca acgacgatgt tttggtcaag
      601 acattcacgt tctttgcggg gactccgcgc ctgattaccg gcagcatatc ggtaggcaaa
      661 agcgatacca ccacagccgt gaccgcacgg ttcaacgaca atttcgatag ttatgaagac
      721 gaatggaaaa ctccggtgtt gacctatgaa atacgcaaag ccgatggcac tatagtaaaa
      781 tcaggaaatg ctacaagaga aaacgacgtc acagaggtga gcctggcatt cgatgatgtc
      841 acgccgaaaa ccgtgtcgcc ttatgaaagc ggtgaattca aaccggcata taccattacc
      901 ttgaaggcga cggcgggtac atcatccgtt tccgccacgg ccaatgtgta catcactcct
      961 ccgccggtga cgatggaatg cctgacggaa acctccaacg ccctggtggg ggaaccggcg
     1021 acttttaatt tccggatcaa gaaccttttg cccggatact cttcaaggta tgaaataacc
     1081 ggcggaggcg gccaagagga aggcggactg acaaaccgga gcgaagaatc cgacccggca
     1141 actgagctca ttaccctgac cggcagcgtc gtcttcacac cggcggtgca gggagacgtg
     1201 tctgagttca gaacgattac cgtctatgcc aataataaag atgagaaaca gcaatccatc
     1261 tcaaagagaa tcaacatcgt caatccgtcc gcagctttga tgagcgtgaa attctatgac
     1321 ggatttgacg gcacagtgat aaatccggaa ccgggagata agccggatgc gttttacgga
     1381 atcagggaga gtcgaatcgc agcacttaca gagggctcca cttataacag cgaagaggtc
     1441 ttcaaatatt tggatagaat gatgcatacc aagacattga gggtggcagt ccccgaggaa
     1501 tggggccgaa tgaaagcaga aggtgcgtca ctcccagcag aggacagaag ggatatctcc
     1561 ctttatctgt cggatgtaga caaaggcaag gagataacac tttcgtggga aaatgttaat
     1621 acccagaaga aattcagttt caacgaggat gacctatccg gaaaggtcta tgccttccgg
     1681 ctactgaacg cactagatga gaagcacgtc agcataagct atacgaactg gagcgaccta
     1741 tcggttacga aaaatgtaat tccttataac gggtatgtga tcatctatga accgagcggc
     1801 ataaagggcg aggtatggat gtcacaggaa ggagctggct acagatttgc gaccttcacc
     1861 cagccaagag gcataagtag aggctacagg actagctggt acgatctgtc acctatggtg
     1921 gtttcaccac tcgccacaga tcacggctat agaagccagc atccggctct agcttttact
     1981 gcaaacacgg cgactatgtc cgataatcct atagcaaagt tcaatagcgt gggcaatact
     2041 gtgccaagtg tcgtaagata ctgcgctata gacaaggaca gaaaaatcat cgctggcacc
     2101 aatggtgtga caaatccatc agcaggcagc ggaaattttg aattgccttt caaggcacta
     2161 tatgaaaatc caaattcgca gctcatggtc gagtatgaat acactacggg caaggagatt
     2221 aggactcagc ttgaatacta ctatttagat aagcttgaag aaaatctaag gcttaaaaaa
     2281 gcgaacagct acgctatcgt cgtatcgagc tatatccaga ggctaagcgt gataggtaaa
     2341 gatggagtaa tacagaattt cccagttgcc gcagatagat ttatcacaat caacccaggt
     2401 gatatcatag aattgcagct cgcaacagcc tcacgcgcag tcaaaagagc agagctgaag
     2461 ctcttgaacc accgccgtac agttgatgca aatggcaata taggcgactg gatatttgat
     2521 tataccaagg catctcctgc ggtcagtata gaagaaatag acctttcagc cttcacgcca
     2581 aacaaataca cgacccttcg cttcttacct acgagggagc agatgacact agggtgcata
     2641 tcacacatca gattagttac tgtgttcgga gacggaacct cagatgcgca taatgtatgt
     2701 tggggacaga tggcagatgc ggagaccgca gcgaatataa agaagataac cgatatacta
     2761 aacgggcagg aattctttca cgaaaagcag atggagttta ggttggggga tttgaatgta
     2821 aaggagaata cccgtaatgt aaagtatgaa gggtcactgt ttacgttcaa tgaggaggta
     2881 gctgcggaaa ttgataaaat aaaaatcaat ttagccagcc cttcaaagaa tcctttcacc
     2941 tatgaagtga agcgcaaggg tgatgaatac actatacgtg gatatgcgaa taccgataac
     3001 atgaacaagg caggcgtggt acaccttgcg gatagatgga aggatgcaac cttcgatgag
     3061 ctgttcggtg aaactaagag gtacgttctg cagtccagtt ctaaatatcg tcctaatttc
     3121 cctggtataa agggttatgt ggaaggcaag gcgatcatta caccaagcgg cgatatacgg
     3181 ttcaccttcc atgaaggtcg cacctatgtc gaatccgact ttttccacaa gccaaagaaa
     3241 ttgtttgact tgagctacta ttgtagttgg actgccgttt ttgaaggtat ggtcacatca
     3301 atatttgaaa taaaagctcc agataatggt ggagacccta cagttccgtt caatttcaat
     3361 ctggaggaat ccagctgggt gtttatgaac acgcgtactg ctcacaatgg gatttcaatt
     3421 gacatagggg tatatgctgt gaaggtttcg ttagaagggg aaattgaaga tgcagactat
     3481 agtcgaaaat ccatccaccg accttacgct acagattcac aaaaaattca atggaatgca
     3541 aggtttagta caggaggcaa tctgtatgaa cgtaactaca atcgtattat tactagtcct
     3601 acagatgtag atatagaaag cagtaatatg aagaaatggt acagctggtt caggaaacca
     3661 ggggaggagc cattttatat aatgcataac atcgaggcta cttccagtgg ttgggggtct
     3721 tctaaggttt cttcctttgg tatgccgtca gatttcactg gtactgatgc ctatagtcag
     3781 ataatgatgg ttgcctcagc ggatgccttt cgcttggcag caagagatgg cgattctcac
     3841 agattggatg tagatgagct atcggcagtg cggtattata atggcggcaa aggcattttt
     3901 tacagcgatt ccagcagcaa tatagtaacc tccggtatag acggcaacgc taccgaaacg
     3961 gtcgatgcga aagcatttgg gatcgacatc gtttcagccg aaaacgggag cgcagtcgcg
     4021 tcctggggga gctacaaagg taatttagac accgatgctc tggacaatgt aggtaaggat
     4081 gggatactca agtacgcatc gggaaaaacc gagatcaaag caggcatttt taacggtacg
     4141 aattggagca ttacgacgct gactgacaat gaaatggcag atataacgcc gaaaacggcc
     4201 gcgaacggca cggagggcgt tgtggtttgg acacagggta ttttgaatga ctaccacctt
     4261 gaaggcaaac tgccggtcat cacgttttca gaaagcaggc ttatgtacgc acgatataac
     4321 ggctctgctt ggggtaagcc cgcagtgtta tacgcacctt ccggcgaaag tatcgcagat
     4381 tactccgttg caatgacaga cgatggcaat gccctcgtaa cggtggtcat gggcagcggt
     4441 aaaatcgcaa ttgtcagaat ctcaaaaagc ggagaggtaa ccgtgattaa caacagcctc
     4501 ccgacaacca cgaaagcaag cttggtatat aatggtaaaa gctatatgct ggcatgctcc
     4561 tccgatgtga aggatgcgga gggtgaggat aatggacaca ttgcattgac tctgtatgag
     4621 ataagtaccg acggtttcgt aacgaatact accttctctg gtataccaac ggatataagc
     4681 ggagatttca acctgttccg caactggtcg gaatcaggtg ctgccgtatt agtatggcgt
     4741 ggttctggca aagataattc ggaagcaagg atcacgattt atgcatccaa agtgtctgag
     4801 gcgaatgaag ataacaccgt attggtctcg gcaccgatcg cagctgccaa catcaatctg
     4861 ccaaccagta caggacctga tggcgaagga ggagcgttta ctgcttccta tgatgcatat
     4921 atcaaaggaa acgacctgaa aatcctgact atattcggtg agtcatcatc tgaggagtca
     4981 aaatccggag agtcaacccc cggggagtcg gaccgaggag tgtatttagc cgaaacacag
     5041 gcacagttta aaaataccat cgaaatcgct tcctgcgtga atgatatttc ctaccttatg
     5101 ccggggctcc agagcaattt taacatcaaa gtcaaaaaca aaggctttgt acccattaca
     5161 agcatcaatg tcaaaatcgg cagcggagag agtgggacga ataccgtgtg tgtcctgcca
     5221 aacgacgaaa ccgcagtcac agccgccttc atacccacgc gggatttacc ggatagcatt
     5281 gattacactg tgactgccgt gtttagcgac ggaagcgaat ccacggtcac agtctccgtg
     5341 agtctccttc aggccgatat cgctgcggaa atcctattgt tcacccagga aaacggcgat
     5401 tataatatca aagcgctcat atcaaataac acaccattct ccctttcagg caaaactgtc
     5461 atcgccggaa tctatcagga tccctttggc acagttccag ttaccgagga gaagattgac
     5521 ggaagtaagt ttgaaaacga gacattgggc acatcgcttc tagttgattt ttcattcgct
     5581 gatgcgaagg atctgccgca gtcgctgtac ctcatagcca aagtatacga ttcttccggc
     5641 aatgaaatac ccgacaacga cagtatgaac aacattctga ccgtaacgaa catcgtgctt
     5701 gtgtcgtcct ccagtcccgg cgacgacgat gacggttcgt cctccaaccc cggcgacgac
     5761 gatgacagtt cgtcctccaa acctggcgac aacgatgacg gttcgtcctc cagcggtggc
     5821 tccagttccg gcagcacaac cactaccata acaaagccag caaagacacc tgaccagcct
     5881 gttacagcgg tggctcctgt gacagccacc gccggagcga atggtgcagc cagcgcagcc
     5941 attccggata aggctatcac cgatgccatt gccaaagcac aggctgacgc caaggttcag
     6001 ggcaaaaccg caagtggtat ttcagtgggg ctgaatgtca acatgccaaa gggtgcaacg
     6061 gccttgacag caagtctgac tcgcagttct ctggacagcc ttgtgaacgc aggggtatcc
     6121 cagcttgaac tcaatggagc gccggtttcc ctcagccttg acctaaaagc cttacaggaa
     6181 atccagaagc agagcagcgg cagcatcagc atcaccatcg ccccggcgac aggactctcc
     6241 aaggaagcaa aggccctcct cggaaacaga ccggtatata acattactat cagctatgtc
     6301 aaggacggca aaaccgccaa tgtaaccagc cttggcagcg gaacagccac cctttccatc
     6361 ccttacacac caggcaagaa tgaggctgtc ggatatctgt tcggcgtgta tgtggacgca
     6421 aaaggcaaag cacaacgcat ccccggctcg gcctatgacg taagcagcgg aagcttgctg
     6481 atccccaccg gccacttctc agtgtacggc gtgggctaca cggctccaag tgccaagttt
     6541 accgacatcg gcaaccattg gggcaaggaa gccattgact atgtagtcgg cagagggctt
     6601 ctctacggca cctcagaaac cacctttgca cccaacattg ccatgacaag gggaatgctg
     6661 gtaacgactc tcggcagact ggcgggtgtg gatgcgaaaa cctataccac aaatagcttt
     6721 atcgacgtaa aagccgacag cgcattccgt ccctacatcg aatgggcgta caagaaaggc
     6781 gttgtgcagg gcatcggcaa ccagcggttt gcacctgacc gggcaatcac ccgtgaggaa
     6841 attgcggtca tcttcgcaaa ctacgccaag gcaaccggct acaagctgcc cgtgacccgt
     6901 aagacaaccg cctatgcgga tgattccagc atcggcagcg tttacaaaac ggcagtcaaa
     6961 gccatgcagc aggcgggcat aatgatgggc ggcagcggca acaggttcaa tccgaaatcc
     7021 aatgccaccc gtgcggaggt ttcctccatg ctcaaccgtt acatcaaact gaccattgat
     7081 tctgccaccg cacagggctg ggcgctgaat tatgccggac agtggctcta ctacaaggac
     7141 ggcaagcccc tcaccggcac gcagaccatc gacggcgtga agtatttctt cgaaaccacc
     7201 ggcgttctga aaaccggctg ggtgaaggat ggcgacaact ggctctacta ttcccgcaac
     7261 aaggccgcta taggctggct ggacatcagc gacaaatgct actactttac caaagacggc
     7321 ctgatggtgt ccggcaagtg gctccagatc gatggcaagt ggtattactt ctacgctgat
     7381 ggcgctcttg ccaagagcac caaagtggac ggctacgagg tagacgaaaa cggcgtgagg
     7441 aaaagtaaat aacgcacacg cataaaaaac aagggtggga accgatgacg gttcccaccc
     7501 ttgtttccag tattcagttt gctttctgca aattgtcccc atgatacagt ggactttatt
     7561 aagacccttt ttttaatatt tctatagttt tacattcccg ataataggct ttgatttcat
     7621 tcagcatatg accctcgaaa ggacgaactt tatttatggc agcctgccac ctcagcaatt
     7681 ttctttaatt catcaacggt gaaactcccc cgcttcattt tttggctgaa agcctgaggg
     7741 ctttccccga aaagccgagc aagctcggca acgcttatat ccagtttcac acataggatt
     7801 tttatctatt cagatactga catatcacca ctttttttga tattctaatt ataaagaaat
     7861 ttgtttagta aatcaacatt aacatttcta ttatattgag aaacgtttaa aaataatatt
     7921 gactacataa acataaacat ttaaaatgta aacagataca aaaaagacga ctcaaccaca
     7981 caaatcaaat atagaggtag cgattacgcg aatagaatac atatttatca ggaaaaatag
     8041 cgagccagta caggtatcaa aaaaaatata caccttgctt aagttaataa acaaacgatt
     8101 tgataagcgg cttaaacaat acaatgatgg cgaaaaacta tgtgactttt caatttagat
     8161 atacagtact aatatagtat caaattttaa aaatagtttt aaattttctt tgttaattat
     8221 tatttagctt atttttattt aagattaata ttgtgatgaa tattatagtt ttgtatggtg
     8281 gttttccaat tataaatctg atatgatttg tattacaata gaaaacatat atctgataag
     8341 ctaggtagcc cactcacctc cgtgatttgt agtatactga aatgcctatg actaataaaa
     8401 taatattcgc tcgtagccaa ccttttttta ttatattttg tgtcttgagc gaagcgaaag
     8461 gaatggatat aattatgaaa atattgattc caaaatgctg tgaagaggtg tattgcattg
     8521 cagcagagga atttaaaagt ttttatgaaa aggtaacggg tactgtatta ccaattatta
     8581 gagagcctca ggatgatgat atgatagtga taggaagtga tgctgtaaat cattatacag
     8641 cggaactgat gctctcaaac aaaatcaacg aatttaaaat cagatattgt accgatgatt
     8701 atcatattaa aactgaaaca gtaaacggca aaaagctttt gtttcttgca gggggcagag
     8761 gtcgcgcggt aatgtatgct gtataccgct tctttgaagt gtgtgctggt tgccgttatt
     8821 tttgggacgg cgatataatt gccaatgtac gtgaaataaa cataaatgat attgatattg
     8881 cggaaagtcc tcactttgag tacaggggtt tgcgttattt tgcacatcgt agccttcatc
     8941 gctttcaggc tgagcattgg agttttgagg attggaaaaa ggaaatagat tggatgctta
     9001 aaaaacggct taatatgttc atgcttcgaa tcggtatgga tgacctattt caaatagctt
     9061 ttccggatat tgttgattat ccttcaaatg atgaaaaact gcctgaggca ggcgaagggc
     9121 atcatgatag aacgactttc tggtctcttg aatacagagg aaaacttaga aaacaaatcc
     9181 ttgagtatgc attcaagcgt gatctaatgc atcctgagga ttgtggaact atgacgcact
     9241 ggtattctat gacaccgtta cagttcctgg agaaaaagaa gccaagtctt cttagtagag
     9301 ataataaata tgaaggcgta gtaacaggca aaatatggga tgtttcatgt gatgaaaact
     9361 taaacaatta ttttaagctt actgaaacac atatacagta ttacgggaaa ccggaaattt
     9421 ttcacaccat aggcttggcg gaacgcatat tttcaaaaga tgcagaaaat aatttaaggc
     9481 ttaagcttta tacataccgc cgtatcacaa ggcttcttca agaaagatat cctaatgcta
     9541 agctgtttct ggcaagttgg gatttatggg gtcagtatcg tgatgttgac gaggtgcaaa
     9601 gacttataaa taatcttaat cctgagcaat caattatact tgattatact tccgatacat
     9661 tacgtagtaa taatttcacg aactggaatg tggttggtaa atacccatat atatttggaa
     9721 tgtttcatgc atatgaaccc aacagtgata tacgtggtaa ttatgatatt acaaacaaac
     9781 gacttgctat tgcttcaaaa gatatttttt gcaaagggat ggtattttgg ccagagcttt
     9841 ctcacagtga cacttttgca cttgagtatt ttgctgataa tgcgtggaat ccgtgtacat
     9901 tatctgtgaa tgaacgaatt aataaatatt gtaatgatag atacgagatg ttttctagta
     9961 aaatgattga aatttggagc aattttatgc caatagcaag cataatgcac tggtgcataa
    10021 atttacgttc ttatatgccg agggaatggt tctttgacat ttttgagcgt ggcaaatata
    10081 tgtttgacag caaaagcaac gttttcgaag aatttataaa aataagaaaa aaaacagaaa
    10141 aatttaagat gaatgccatt tatgcattag aacttgctgc agaaattatg aaagatgata
    10201 caaataatga gtttttaaat agagatgtat atgatattgc acgtactatt tgtggaagat
    10261 acttacaatg tcaactcata aataccgccg aagtattttt aaaatgccat agtacaagtg
    10321 gcacagaagg taattacaga gatttattgg agagtaaata tagaagttgt ataaagctaa
    10381 tggaaatttt aattgatatt cttagtggac atgaggatta ctctcttttt gagtctttta
    10441 aaaaagtgaa atcagtagaa agtgttaatc ctgtttttga aacaacactc aaaaacaatg
    10501 caaccggaaa ttactgccgt tcttacattt atgaaaatgc aaaatattta tatatgcctg
    10561 aaatgaagct atttttcgag tggcttgata aaatacggga ggaaaacagc ataaaagaat
    10621 tgccttattt tgaattatat tatgaaaaag ctaaagaaaa tatacaaata ttttttaaaa
    10681 ctcctcttgc tgacatgatc cctaagtata aaaagaatat tagtgatatc cttattaatt
    10741 tatcacagga attaaaagtg atatagtgaa agagatgaag gaatttgaaa attgcgggcg
    10801 gggggataga taacatgatt aaaatgcaaa ccagccggaa aactggtcaa aaagtctcac
    10861 agaaaatcat tcaaaaaact gataatcaaa ttgatcaatc tatgatatca aggagaaacc
    10921 attctcttct aataaaagga atttttaaaa attatgagct ttaccttctt tccctttcag
    10981 gattgatata cttgattgtt tttaagtatg ttcctatgta tggaataact atagccttta
    11041 aggatttcaa tatatataag ggaatattcc agagtccatg ggttgggttt gaccatttta
    11101 taaggttgtt tacttcagcg aaatttctgc aggtttttac aaatacgctg ctaattagta
    11161 tatataaact tatttttaca tttcctgctc ctataattct ggctcttttg ttaaatgaaa
    11221 tcagacaact tgtatttaaa aaggctatac aaacaatatc ttacatacca cattttattt
    11281 cttgggtcgt tgtaagcggc atgtttttag atattctctc tcctaataca ggaatcttaa
    11341 ataaattaat taaaagtatg gactttgagc caattttttt catgggtgat aaagcttatt
    11401 tccgttcagt gcttgtggtt acccagatat ggaaggaagt agggtgggga gcaattgtat
    11461 accttgccgc aatagcgggg ataaaccctg aactatacga ggcggctatt gttgacggtg
    11521 ccagcaaact tcgtcaggta tggcatatta ctcttaatgg aataaagccc acaatagcta
    11581 tacttctttt attgaggata ggaaatctgc tgaatgccgg acacgaacaa atattgacga
    11641 tgtataatcc agctgtatat gaagtagctg atgttcttag tacatatgtg tttagagttg
    11701 gcttgggtca gatggaatat agttttacaa ctgctgtaga tttatttaat aatattatcg
    11761 ggtgtgtatt acttttttca acaaatcatc tagtacgtcg tcttggggaa cagggaattt
    11821 ggtaaacctt gtgaagtatt ctaaaaatga aaattagctt ggaagggagt agtaaatcat
    11881 gcttatcagg tcaagaggag aaaaaacatt tgaggtgttc aatatattta taatgatatt
    11941 gctggggatg gtaactttat ttcctttaat acatattgcc agtaaatcgc ttagtgatga
    12001 ggcttatgta cttgcaagag atattgtact atttcctaaa gggcttcaat ttgaagccta
    12061 tagaaatatt attggcaatg ccagatttgc gaggtcattt caaaacacaa taatacttac
    12121 atttgttggt acctcaatca atgtcatgat gactgttata actgcatatc ctctttcgag
    12181 aaagcgtttt cccggacaat ctgcaataat ggtattttat gtttttacaa tgtttttttc
    12241 cggcggaatt attccatctt acttgcttat gagagatctt aaactgcttg attctatgtg
    12301 gtctcttatt ctaccaggaa ctattaatgt atataatatg atacttataa aaaatttttt
    12361 tatgtcagta cctgatagtc ttgaagaatc agctaaaata gatgggtgca aacaaatccg
    12421 tatcctattt tcaatatttt ttccgctttc tttaccagct atttctacca taacattatt
    12481 ttatgctgta ggtcattgga attcctactt tgctgcattg atgtacttaa attcacgaaa
    12541 taaatatcct ttgcagttat ttttgagaga gatgctgatt gaagaaactg tggatgttgt
    12601 tgatatatca aatctacttg atgtagcacc tgactcagtt aaggctgcta caataattgt
    12661 tgcaacggta cctatacttg taatatatcc attcctacag aagtattttg taaaaggtgt
    12721 tatgataggg gcaataaaag aatgattaga aataagtact atatataact taccaaagat
    12781 ttagagatat agcaggatgt tatatcttga aataaaagct tctataataa tagctaacca
    12841 aaactaaaaa gggggttttt gtatggaaaa aatattttct accgcagtaa ctaaaaaagg
    12901 gggtttttgt atgaaaagaa tattatctat catagttgca cttatgattg taatattgac
    12961 ttttagtggg tgtgttacaa aagatgctgg aaaagctgaa caaaaaacgg gtaatactga
    13021 agcaacacag aaaaccgagg aaagaccaaa gataaaaatg cttgttgatt acaatattgg
    13081 tgtagctaat tttaaagctg atgaaaatga aaacaaaaac ataatttacg actggataat
    13141 agaaaattca ggttttgata ttactttcga agtaaaaccg aaagataatc cagaccaaaa
    13201 ggtgtcaata ataatggcct caggaaatgt tccagatata ttggagattt ccaataaaac
    13261 aatgtactcg aattttgcaa gccaaggtgc gttgactgat gtaacagaac ttgttaaaac
    13321 agctccgaat atacaatctt taattcctaa agatagcttt aaggctatag aaattgccag
    13381 taaaatttat gcggtgccaa gacctccaga gaatgaaggt tatggtcaag gtatctatgc
    13441 aagattagat attttgaaaa acaatggtct ccaaaaccca aaaaccaccg atgaatatta
    13501 taatgtactt aagtctgtca cagataatga aaaaggagta tggggaatta caagtgatgg
    13561 tccttttata ggtgggttca tgggagcttt tgggatagca gcaaaaatga aggaaaagga
    13621 cggtaaagta gtttatacat atacgcagcc tgaatttaaa aactatttga cttatataaa
    13681 aagactttat gatgagaagc ttcttgatag ggagttttca gtaaataaag gttctgcaaa
    13741 agaaaagcta atatccgaag aagctgtaat ggctttttca gatttctctg tttcaaaact
    13801 tgtttttgag aatctaaaat ctaaaaattc atctgttgaa ttaggttact tagatcttcc
    13861 tataggacct gatggttctc aaggtgttcc aaagtctcgt cctgttataa ggtggtttgc
    13921 aataccaatt caatctaaaa atgctgaagc agcaatgaaa tacatggatt gggtttgtcg
    13981 ggaggatgtt caaaaagttc ttgctctagg ttttgaaggt gtacattata atatggttaa
    14041 tgatatacca gtgccaacag aaaaaatgcc ggatattgta tggagagtaa tatatcaacg
    14101 actggagaac gggacatttc ctatgaggat tcagacaaag ggtttctggc ctttctacga
    14161 acctacgtta aagaatccgt tagttgaaaa tattgccgaa tttgtgcccg ctattgaagc
    14221 agttgacaaa aagtctaatg aacttcaaga ctatgttgtg gagaatagca taaagtttat
    14281 taccggagag aggcctctat cggagtttga tgctttcgtg caagaatata acaaacttgg
    14341 tgctgaagaa agtatgaagc tgataaatga atggtatgct gaatttagca aaaaataact
    14401 actgaaataa ctacaaacag atatcaagcg ctgcactttt gtgtggcgct tttcgaaata
    14461 tggtgcacct gagcgtttta gctgataata cataatgcag taagcacagc ccttgaggtg
    14521 taggttccgt aaaaaagaac cgatagatca ggtacctatg aggtacaacc ccacatagga
    14581 gggtggagtg gcactactta aggtttcatt gatgaaggtg ggtcggaaac ggaacaactg
    14641 gagttcattt ctcctccact tgagatagga accaccgaac tttcacatag aagcacctca
    14701 atttattgag gtgaggacat cactgttgac caaaaacctc ataaatatta caataaaaga
    14761 aaataacacg atattacata caaattgggg gaaatctctg atatgttaaa tgtacttata
    14821 gtagatgatg aaaaggctac acgggatgga ctggcacagt gcattgattg ggaaatgtat
    14881 ggtatgactg tgatcggaga agccgcggag ggtaaagaag ctctgaagat tatggataga
    14941 atcccagtgg atattgtagt atccgatgtg gttatgcctg ttatggatgg tatacagctt
    15001 gtagctgcca taaataagaa caagcctgat acaaaggtta tattggtaag tggttattct
    15061 gatatacctt acctgaaatc ggccataagt ctgggggctg tagattatat ttttaaaccg
    15121 gttaaattaa aagaattaaa ggctgtttta tcacggattt caagtcattt cgaaaaacaa
    15181 atggagcaaa agaaaatttt taatgaaatg aaaaacaaac tggaacagag cataccctat
    15241 ctaagagaac gtttcattac taagctaatt tccgggaaaa ttcaggatga cactaatata
    15301 cttgagcaaa tctctttttt aggtatgaat cttaacctga atggttattt ctcagtgatg
    15361 acggtttcaa tggaatcgga acatgaggct ggcaggaaat tagctggtga taatgaactg
    15421 tataaaatgg ttggcctatc ggcacttcag gaatactttg atgcaagtac caggatctgg
    15481 tctatgtcaa ttgatgaagt taactttgtg ctgcttttat ccactgacta tactatcgat
    15541 tataatatgt cactaaaaat tgcttctgaa atccagcaga ttatcaacca atatacagga
    15601 tatagtatat ctataggaat aggcagtgag gttgcaagac taacaaaagc atatatatct
    15661 taccagcaag ctataagagc actggaacaa aaattttttc ttggagaagg tcagattatt
    15721 cattttaatg atatacagaa tgtaactttt agcaaaggct tttaccctga aggtattgta
    15781 gatgaaataa tagatatggt aaggcttgga aacaaagaaa gagtagaaat tctagtagat
    15841 agaatgtttg aagacatatt tcagaattcc tgccttaact tgaagctggt gcagaatatt
    15901 tgctttgagg cggttacact ggcaaacagg cagcttctgc aagctcgtgc aattgatgaa
    15961 tctcacgaag ctggactttt ggacatgtct actctgaaag atatatttaa gccaaaaagt
    16021 ttgaaggaaa cacatatttg gctaaaaaat ctattatatg atgctgctga aagaatttgc
    16081 acagtgcaga gcaatatttc aaagaatata ataatagaat ttattaaaaa ggctataaac
    16141 gataaatacc atgaggatat atcattgtta actatggcgt tagaattttg cttaactccc
    16201 aactatctat gtcttatttt taaaaaagaa acaggtgaga cgttcaatga ttatcttaca
    16261 aaagtgcgtg tgcaaaatgc aagagaattg ttgaaagatc cgacatataa aatatatgaa
    16321 atagccgaaa tggtaggata tcgtgactct gactattttg ccagggtatt taagaaaata
    16381 acaggattta ctcctagtga atttcggaaa gggttgatcc tatgaaaaag ctttcaaaac
    16441 ttacacggag cacgcggctg agagacagat ttacattgat ttatattttt tttctagcaa
    16501 ttccaatgct ttttgtatgt ctttatttat atggccagtt aagtataata tctgagtatc
    16561 aagggcagca agtagtagaa cgtgctatca ggcaaagcgg ggccagtatt gcatcacttg
    16621 ttgaagaaat agaaaatgtt gcaggacagg tatttatcaa taccactttg gtaaaatata
    16681 ttactgatga ggattcctcc gatgctaacc agtattctga gctgaagtat atagaaaaca
    16741 taatcgacca gattctttac tatccgcaag taggttatgt gcgtatgtat atcccttcgt
    16801 acaagctcta cgcaagaaat tatgcaataa tgtttcctat agaggatatt atggatactg
    16861 aaaattataa acagcttatt ggaagggaaa gacaaattgg atggacggac agttattcat
    16921 atagtatcaa agatcagggc agcagagatg ttataactta tatggtagca ttgaaggatt
    16981 ttacacaaat agctcgtata gtaggatgcg tgtgtattga tattaagcag tctacattta
    17041 ttgaaaaact taaagtaaca attattggtg ataatgctgt gtcattgttg ctctcagata
    17101 gaggagatgt tatggcatct gcttcaggag attactgtaa acttgatacg aatcttattg
    17161 atgaaattat tttatcttat cgtggtaagg ataatgttga ttttcatgaa ctaaacggga
    17221 aataccttac ggtgaaagtt gatggcaaaa aatatgttgc catatcacat acaataaaaa
    17281 gcaatggctg gaccattgtg tacaccatgc ctcattctga tatattagcg cgcagtaaga
    17341 agctaatgaa tactataatt ataacagtca tgtttattat aacaataagt gtaatgtctg
    17401 ttgtaattgt tacagacaga ataacaaagg gaattagcag ggtaatatcc aaaatgaatg
    17461 atattgatat agtttaccat aatgtttctc ctcatatgca gattgataat gtgagttata
    17521 acgacgaaat aggcagcctc cagaaaactt ttaacgggat gatatcacga atataccaat
    17581 taaacaacga gatatatcag acgaaaatat taaaacggga ggctgagctt cgtgctctac
    17641 aggctcagat aaacccccat tttttgtata atatgctgga ctctattaac tggatggctt
    17701 taaaagcagg taatgatgct atcagtgatg tggtcagcga cttgggaaga ttttaccgta
    17761 taagcttaag cgggggaaaa gacataataa caatagctga agaattggaa catgtaaaac
    17821 tgtatatcgc tcttcagtca aaaagatttg aggatgctat aactgtcaat tataatgtag
    17881 acagtactgt aatggggtta aagatggcaa agttaacatt acaacctctg gttgaaaacg
    17941 ctataataca cggtattatg gaaaagcctg aaaagaaggg gcaaatattt atcaatgctt
    18001 atatagagag tgatatagtt aagatagtgg tcactgacga tggagttggg tttggattaa
    18061 aaaatcaaaa tgatgtaagc ataaataatc atcatggaag cggatatggg ttgaaaaatg
    18121 tagatcagcg tataaagctg tactttggtg acaattatgg tgtcaggctt tatagaagca
    18181 gtgacatgtt gactatagcg gaaatatctc tcccagcaat tgaaatgatt tagtctaatt
    18241 taatttagca actcaacttt cccagccaat acaagctccg ggttcttatt ggattctctg
    18301 atgcctacta taaaaaagca gatactttgc tggtagacct tttactgaat ccaagtgctt
    18361 aaagagtgtt tcggtagaat tcactgttgc ccctgcaaat ccaagcaaat gccgatcttt
    18421 cactctgtca gatgtcataa actttataac atccaagccc cttgaaagac attctttata
    18481 ccaactattc acactaaatg ctatttgtct ttttctcatg aacagcccta ttggtagaaa
    18541 gtaaaattca atgtgttttt cataaatgcc aagtgtatag tcgatattct tcgatttacg
    18601 caaagccctt ttcgccgcag ccactatgct acaagcatta tatatttcac cgttcatgtt
    18661 gtttcctttc gatgcgtatc aaacgtagtc tctgcccgct cggcttcaat taacagatag
    18721 ttataacacc agttatcata ttcatccttg gtggtttcac cgcgtttgaa cttatcatac
    18781 tctttccgcc ttgcgctgaa catatcgaac atggaactta gagagcggtt tatcgctata
    18841 aggactggat aggtgagcga aaacaaacac aacgccgcag atttctatta aataaagatg
    18901 taatcaaatt tgtctttatt acacaattga acttgcagat gaaacacaga aggttgctac
    18961 cagtataact gttgcactaa taagggctta aatggctgct gagagtgtga tatagctcct
    19021 tgtgacattg gtatgatgaa gaatatagtt tcaaaatcac agaaatgaat aaaattactt
    19081 gttaggactt atatacttaa ggtttgcaga tagcaaatat ggtaaatatg aagaagaaat
    19141 aaataaagaa tattatgagt taaaaggcaa cagatggaaa aagatagaag gtatatacga
    19201 taatatctgc agagttagaa aagaacgagg tataggtcta acagtttgta cggctattgc
    19261 aacatgatga cgtcgttatg actagagatg cgctaaatac aacatatgat gagttgttga
    19321 gacagtaaaa caatattcgc tgtaaaaagt gttttatttt cattattatt cattgcaatt
    19381 aatgaaactt tgtgctatcc tatagtagag gtgatgcaca tgtatagaga cgcaatgaat
    19441 gatttgcata agtggaaaag caaaaaaaat aaaaagccac tgattattcg tggcgctcgt
    19501 caggtaggaa aaacatggct catgcgcgag tttggcagtt ctgcttatga tgagatggta
    19561 tatattaatt tcgataacaa cactcaaatg aaagagctgt tttctattga tatgcgtatt
    19621 gagcgtatta ttacaggttt ggagctttat gcagggcata aaattgaccc gacaaataca
    19681 ctgtttatat ttgatgagat acaagaagtg ccgcaagctt tggcagcgtt gaaatacttt
    19741 aatgaaaccg caccggagta tcaaataatc tgtgcaggtt ctttgcttgg tgtggcactt
    19801 catagtggca catcttttcc cgttggaaag gttgagttct tggaattata tcccttgtcc
    19861 ttcaatgaat atatacgagc aatgggcaaa caacagtttg tcgagctgct tgaaaaacag
    19921 gattttgaaa tggcgaccac ctttaagcag gagtatatag acttattaaa gcattactac
    19981 tttgtgggcg gcatgcctga ggtagtgaag actttttcag acaataggga ttttaacgaa
    20041 gcacgtgaaa ttcagcaaag gatactggat gcttatgagc aggatttttc caaacatgcg
    20101 ccaaacgaga ttgttccctg tattcgtatg ctatggaaca gtattcctgc acagcttaca
    20161 aaagaaaaca agaagtttat ctatggtatt attaaagagg gcgctagggc taaagattat
    20221 gaaatggctc tcatgtggct agctgattgc gggcttgttc ataaagttca tcgggtaaaa
    20281 gcgccaaata ttccgttaaa ggcttatgag gacttgaaag catttaagct gtttttggtt
    20341 gatgttggac ttctttcctg catggtacgc cttaatcaaa gggttttgct tgataggaat
    20401 gggctattca aagaatttaa gggagctctt accgagcagt atgttctaca gcagcttaaa
    20461 acaattaagg gtatagaaac ctattattgg acaaacgacc gtggcagcgc agaaatagac
    20521 ttccttatcg acaacggaac agatgttatt ccgattgaag taaaagcgga aacaaatttg
    20581 aaggcgaaaa gccttaaaac tttttgtgag aaatttgagc ctggaatggc gattcggaca
    20641 agcatgtcag attataagca ggaagattgg ttattaaatt taccgttgtg ggcggtggag
    20701 atggtgaata attaaataag caccgtaacg atagcccctg gtaaatattt cttcagattt
    20761 gataaatgca ccctattgca atatttcaat tgacaaacag cggacaagtt tggtagtatt
    20821 attttgggga ttcaagcgtg gcttcctagg ctttaccggg ttggttctac cagcccgttt
    20881 ttgcattgaa ggtgtaaaac tgtataatta tatcaataat cgtcataatt agttttatga
    20941 tgactttatt aatttaagga ggttatatta tatggaaaag ctttttaaac taagagagaa
    21001 tggcacaaat gtaaagactg aaataattgc cggcatcaca acttttatga ctatggcata
    21061 tatccttatt gtaaatccca atatacttgg agacgcaggt atggaaaggg gcgcggtttt
    21121 tacagctaca gctgtttcag cagcaatagc taccatacta atggcgtttc ttgccaatta
    21181 tcctgttgct ctggcatccg gtatgggact aaatgccttc ttcgcttatt atgttgtagg
    21241 cgtcttggga tattcatggg aaatcgcact tacagctata tttattgagg gtattatatt
    21301 tgtcatttta actctgttca gattcagaga agctattata aactctatac ctgcaaacct
    21361 gaagtatgga attacagtgg gtataggact atttattact ttcatagggt tccagaatgc
    21421 aaagattatt gtcaataatg atgcaacact ggttggactt ggagacctta agagtcttac
    21481 agtaattcta agcattattg gtattttagt tactacttta atgttacata agaaagttaa
    21541 gggagctctt ctctggggta ttctggtaac ttacgtactg ggtatcatat gtcagctgac
    21601 gggcttatat gttgttgatc cagctagtgg aatgttcagc ctgatacctg aaaagatcat
    21661 ttctttacca ccgtcagttg caccaatatt cttaaaattt gattttaaag gtgctttggc
    21721 tcttggaaca aatttcttcg tagtaatgtt tgctttcctt tttgttgatc tttttgatac
    21781 agtaggcacc ttaattggtg ttgccagcaa ggcaaacctt ttggacaaag aaggcaaact
    21841 gccaaaagcg aaagaagctc ttatggcaga tgcaattgga actgtagccg gtgcatgcct
    21901 tggaacttca actgttacaa gttatgtaga aagtgctgca ggtgttgccg aaggtggtag
    21961 aacaggatta acagccctta caacagcggc tttgttgatt gtttcattaa tattttctcc
    22021 tattcttaca attattcctt cttttgctac agcaccggca ttaattatag taggattatt
    22081 catggtttca gcaatttcta aaattgattt tacggaagat tttactgaag ctctgccagc
    22141 tttcctggca atgatagtta tgcctttgtc ttatagcatt gctgatggta taatggctgg
    22201 tataatttca tggtttgtat tgaaacttat atcagggaat attaagaaaa tacatccagt
    22261 tatttatgta ttggttgctc tttttatttt gaaaatagtt gcgtaaattt gagcaagatt
    22321 tattgtatgg cagtgtagtg tagtcacagc ctgcttgagc cggtgaagtc cgatagctga
    22381 actatcagac ttcaccgata tcccgatatc ccgataccct gattagtata ggcatattag
    22441 caaagaatat tcagcaagga gtatcctgtg acatattttg aaatgcatgg gaaaatgtgt
    22501 atgctgcaga gcagatggtt taatttgtct cattaaacaa cctgctgtga ggcatataat
    22561 ataaaagttc taattaaaaa gaaattggta ggtatggagg attctataaa ttatggaggg
    22621 ttctatatat gaataatgga agattaaaag ttgctgtaat tatgggaagt gattcagatt
    22681 ttcctaccct taaaggttgt attaagatac tgaaggattt tggtgttgaa tataaggtaa
    22741 tggtttgctc agctcacagg actccggaaa aagcagcgga gattgcgaag aatgccgaat
    22801 cagaaggatt tggtgtaatt atcgctgctg caggcaaagc tgctcatttg ccgggagttt
    22861 tagcagctta taccccaata ccggttatag gagttccaat aaaagcttct acccttgacg
    22921 gattggatgc actattatcc attgtacaaa tgccgacagg tattcctgta gcaactgttg
    22981 ccatagacgg tgcagacaat gcagcactat tggctataca aatactatct gtagccgata
    23041 atgaattaag gggcaagatg aagaactata aagaaaagct ggcgtcaaaa gttgatgaaa
    23101 aaaacctggc tcttcaggag atacttagta tggaaggtct atgacaatta gttttgttat
    23161 gttgccaatg gaaaatgcta tcaaaaatta tcccgaaaga cattgctttc caaattggca
    23221 ttgtagtatt aaaagaaatt taaaaactta tgccgaagta cagcggcgga atggtggttg
    23281 taagagtata tgatatacga cattaatgat aaaatcaggg aagaatgcgg agtattcgga
    23341 atacttgata acgatggaca caatgtggct cagcttactt attacggatt atttgccctg
    23401 cagcatagag gacaggaaag caccggtatt gcggttaatg actcaggtac aattatttat
    23461 tataaagata ttggacttgt acctgaagta tttaatagca caatactgga tcatttaaaa
    23521 ggacaggcgg caattggtca tgtgctgtat tctcctgata gcagtaatag agagaatgta
    23581 cagcctattg tattaaaata caaatcggga caaatggcca tatcatataa cggaaatctt
    23641 ttaaatgcat ccaaactgag gcaggatatg gaggaaagag gagcaatatt tcagacaaac
    23701 agtgatgcag agctaatagc aagtttgata tcaaggtata ggataataag tgataatatt
    23761 gaggaagcat taattaaagt aatgaaagat attaaaggct cctatgcatt tgttattatg
    23821 acacccaaaa aacttattgc aataagggac ccactcggca tgaaaccact ttgtataggg
    23881 aagcttgaca actcctatgt tgttgcatca gaatcttcag ctgttgatgc agtaggaggc
    23941 aaactaataa ggaatgtaaa cccgggagaa atcgttgtaa ttcaggaaga tggcataaaa
    24001 tctattcaga ctgaagttac aggaaaaaca aaactatgca tatttgaatt tgtatatttt
    24061 gcaagacagg acagttatat tgacggcgca agtgtttaca gagcaaggct ggaagcagga
    24121 cgaaggcttg ctattgagca tcctgtagac gccgatctag ttataggtgt tccggacagt
    24181 ggattgattg cagctcttgg ttattcaaga gaatcaagca taccatacgg ctcaggcttg
    24241 ataaaaaaca ggtatgtagg aaggacgttt ataaaacccg ggcaatcaca acgtgaagaa
    24301 gcagtaagaa ttaaattgaa tgttataaga aacgaaatag aaggaaaaag gattgtaatg
    24361 gtggatgact ccatagtcag aggcactaca acaaaagcta tagtgcatat gctgaaagaa
    24421 ggaggggcaa gggaagtgca tataagaata agttcggcac cttataaatt cccatgccac
    24481 tttggaatag ataccccttc cgcaaagcat cttgttgcat caaactattc gctggaagag
    24541 ataagaaaaa tggttggtgc agacagcttg aaatatttaa gccttgacgg attgcttaaa
    24601 acacctatag gatctgattg tgacttttgc tgcggctgtt ttaccgggga ttacccactg
    24661 aaggtagaat aagtgctaga ggagaagaaa gtgtaaaaat cttagaacat tcagaaagtg
    24721 taaaagtata gaaaacacag gaatcgtggg aagtgtataa gcacagaaag aataggaagc
    24781 ataggaaatg tgggaagtgc aggaaatata agaagtatag ggaatataag agatatatta
    24841 agtgtgggag atatgtcgga agatatataa ggaggagaaa aataaatgac aacatataaa
    24901 gatgctggtg tggatgtaga agctggatat gaagcagtta gacttatgag ggatcatgta
    24961 aagaggactt tcagacctga agttttaaca gatataggag ggtttggagg attattttct
    25021 ttaaataagg aaaaatataa tcaacctgta cttgtttcag gtactgatgg agttggtaca
    25081 aaacttaaaa ttgctttcct tatggacaag catgatactg taggaataga ctgtgtagct
    25141 atgtgtgtta atgatatagt ttgcagtgga gcagaacccc tgtttttcct tgactacatt
    25201 gcagtaggga aaaatcgtcc tgaaagggta gcagaaattg ttaaaggggt atctgaaggg
    25261 tgcattatgt ccagatgcgc actggttggc ggagaaaccg ctgaaatgcc gggtttctat
    25321 tccgaagaag aatatgatat tgccggtttt gcagtaggta ttgttgacag agacaagatt
    25381 attgatggta aagacattca agaaggagat gaacttatag gtcttgcttc ttctggaatt
    25441 cacagcaatg gatattctct tgtgagaaaa ttgctaaacc caactccaca gaaactgcat
    25501 gagtatgtgg aacttttagg tggaacactg ggtgatgaac tgttaaaacc cactaagata
    25561 tatgttaaaa cagtacttga tttaattggt aggtttaaaa taaaaggaat ttctcatata
    25621 acaggcggtg gattcattga gaatattccg aggatgattc ctgaaggcct gagagcagaa
    25681 ataaatatag gatcatggct ggttcttcca atatttaatt tgcttaaaaa ttccggaaat
    25741 attgacgaaa aagatattta taataccttt aatatgggca ttggaatggt tatgactgtt
    25801 gaaaagggta cagccaggga aataatagat tatctcaata gagatggtga gcaggcatat
    25861 ataattggta aaattgtcag aggggaagcc ggggtggagc tgtgctgaaa attggtgttt
    25921 tggtatcggg agggggtacc aaccttcaag ctattattga taatattgag tcaggagatt
    25981 tgccgggatg ctcaatagct ttagtggttt ccacccgtgc agatgcctat gctgttagaa
    26041 gggctgaaaa gcataatatc cctgctgttt gtatatcaag aaagaatttc gaatccatag
    26101 accagtatga tgaagcctta ataaaccagt tcgaaaaata taatgtagaa ttgattgtaa
    26161 tggctggatt cctttctata gttggagaaa agtttgtaaa taaatattcc ggaaggataa
    26221 taaatgtgca tccttcatta ataccggctt tttgcgggaa aggatattat gggctgaaac
    26281 ctcatgagaa ggctctggaa tatggtgtta aggttacggg tgcaacagtt cattttgtag
    26341 aacttgagcc tgatgcagga cctattatat tgcaaaaggc agtttacata aacgatgatg
    26401 atactcctga agccttacag aaaagggtta tgcaggaggc agaatgggag atcctgcctg
    26461 atgccataaa gctttttgct gaaggtagac tggttatcga aggaagacga gtaaaggtga
    26521 aaaaaggagg tttttaatct tgattaaacg tgcattaata agcgtatctg ataaaacagg
    26581 tattatagag tttgcaaaag gattggaaaa gttaggagtt gagataatat ctacgggtgg
    26641 aactgcaaaa gctttgaggg atgcagaagt taaggtaact aatgtgtctg atgttacagg
    26701 tttccctgaa tgtcttgatg gaagggtcaa aacattgcac cccaaaatac atggcgggct
    26761 tcttgcaata agagataatc aggaacatat gaagcaactt aaagatcttg ggatagagcc
    26821 tattgatatg gttgtaataa atttatatcc ttttaaaaaa accattgaaa aagaaggagt
    26881 atctcttgaa gaggcaattg aaaatattga tattggcggg ccaaccatgc taagagcagc
    26941 agctaaaaac tacagggatg tagctgttat tacagacccg gcagattatg acaaggtttt
    27001 ggcggaaatc agtaaatcag gagaagttag ccctgaaaca aaatttttac ttgcttacaa
    27061 ggtttttgaa catacatgcc attatgatac attaatagct cactatttca ggggacagat
    27121 gcttaaaaga aatcaggcta ttgctgcaac ccatgatatt agtaatagca acctgaaagc
    27181 aggcagcaat gatagccagg aaaaaggtat atataatatc gaagaattct ctcaaacatt
    27241 aactcttacc ttcgagaaaa ttcaggatat gaggtatggt gaaaatcctc accaaaaggc
    27301 cgctttttat aaagaaccgg gtaccaatgc aggttgcctt acaaatttgc atcagttaca
    27361 tggtaaggaa ctctctttta ataatataaa cgatacaaat ggagcactgg acttgctgaa
    27421 agaatttgaa cagccaacag ttgttgctgt taaacatgca aacccctgtg gtgtggcaag
    27481 tgcagatagc atctatgaag cttatcttaa aacttatgaa gctgatcctg tttccatatt
    27541 tggaggcatt atagctgcaa acagagagat agatgcaaaa accgcagagg aaataagcaa
    27601 gatatttgta gaaatagtaa tagcaccatc atacagtgaa gaagcattta atatcttatc
    27661 cagaaaaaag aatatcagaa ttcttaccct tgatgatatt tcaaagaaac tatctaaaga
    27721 ggcatatgag ctgaaaaaag tttcaggtgg tttattagta caggaacgtg atactgagct
    27781 tataaacata gatgaaataa gctatgttac caataagaga ccgggagcta aggagatgga
    27841 ggacttgatt ttcgctatga agattgtcaa gcacactaaa tccaatgcaa tagtgctggt
    27901 aaaagacaag cagacagtgg gcatcggtcc cggccaggca aacagagtta catcagtaag
    27961 aattgctctt aattacgcac aagaaaaggc aaaaggagca gtattggcat ccgatgcatt
    28021 tttcccattt tcagattggg ttgatgaatc tgtctctgca ggaataactg cggttataca
    28081 gccgggaggc tcattaagag accaggaatc aatagatgcc tgcaatgaaa agggtatagc
    28141 aatgatattt accggcatga ggcattttaa gcattaatgt caacaggagc ttagttttaa
    28201 agaaattgtt aaatttctgg aaaactaatg cgcatgtgca tatttttttg cacaggaatt
    28261 atgaaaaaac aaattgcacg tgcagttgaa atgaatttaa gcccatagaa acttttttgt
    28321 aaaaaagttt tgttcctgca aggacgaatt catttcgcgc gctgcaggaa tggagccagg
    28381 aatggagttt aaaatgaaag ttcttgtagt tggcggagga gggcgtgaac atgcgcttgt
    28441 atggaaagcg gcccagaatc cgtctgttga aaaaatatat tgtgcaccag gtaatggcgg
    28501 catagcaaaa attgcccagt gtgttcctat taaagcaacg gatattaaag gcatggtaga
    28561 gttttcaaaa tctaacaata tagaccttgt gattgtgtcg cctgacgacc ctctggccgc
    28621 aggaatggta gatgctttac aggaggcagg tataagggct ttcggacctt taaaaaatgc
    28681 agctgttatt gaagcaagca aatccttttc caaaaatctt atgaaaaaat acaatattcc
    28741 gacagctggg tatgatgtgt ttgataattg tgataaagcc atagcatatc ttaaagaata
    28801 caaataccct gttgttgtaa aagctgatgg attggcagct ggaaaaggtg taattattac
    28861 agaaacatat gaaattgctg tggaagcagt taaaaatata atggatgata aaattttcgg
    28921 cgaagcggga aacaaggtta ttgtggaaga gtacctaaca gggcatgaag tatccgtatt
    28981 ggcttttacc gatgggaaaa cagttatccc catggttagc tctcaggatc ataaaaaggt
    29041 ttttgataat gatttgggcc ttaatacagg tggaatggga gcagtttctc ccagtaggat
    29101 ttatacacct gaaatggcag attactgcat gaaaaaaatt atcaggccca cagttgaagc
    29161 tatgaataaa gaagggcgca cttttaaggg agtgttgtat tatggtttga tgattaccga
    29221 aagcgggccg aaagtgctgg agtacaacgc aagatttgga gacccggaaa cccaggttgt
    29281 tctgcccaga cttaaaactg atattattga tatttttaat gcaataatag atgaaaaatt
    29341 agatgaaata aacattgaat gggatgaaag agcagctgcc tgcgtgatta tggcttcagg
    29401 gggataccct gggaaatatg aaacaggata tgaaattaag ggcctttgtg atgcacaaaa
    29461 agataaagat acaatagtgt tccatgccgg tacaaaatat gaaaatgaca ggtattatac
    29521 atccggtggg cgggtgcttg gggtaacagc tttaggagaa actcttgaaa tagctgtaaa
    29581 aaaagcatat gaaggagtat caaaaataga gtttgaaaat atgcattacc gaaaagatat
    29641 aggacgaaaa tagactgccg gctaccctta attaagtttt aagggactag aaggggctcc
    29701 aatgagactc taaagagacc caaaaagatt ctataaagtt gcagcctgta aaagaaaaat
    29761 aaaagtcaat aaaggaaagt ggtaattttg aagggcaaat tcaaaaggat aggtatttcg
    29821 ggggggactt tcgatcctat ccattacggg catttaataa tagctgaaga aataagggaa
    29881 acaatggagc tggaaaaaat ggtattcata ccatcagcca atcctcccca taaggtcgga
    29941 attggtgtaa caaatgccac acatcgctat aatatggtaa agatggcaac agagtcaaac
    30001 ccttggtttg cagtgtcttc tatagaaatg gaaaggggag gttattcgta tacagttgat
    30061 actctcgtac agttgaagca gatatatggc gaagatactg tccttttttt tatgacggga
    30121 gcagatgtaa tacctgaact tgtaacatgg aaaaactttg aaaagctatt ttccctatgt
    30181 gaatttgtag cggtattaag acctggattt aacagagaat cattgattaa agaaattgag
    30241 tttttaaaaa gtcattttat ggctaaaata catatagtgg atgcgcctct aattggtata
    30301 tcatctacga taatacggga aaaagttaga aatggcaaat ccataaaata tcttgtacct
    30361 gaaaatgtag aggagtatat aacagaaaat ggactataca aataagtgaa cattttgcct
    30421 gcattttcga attgttcaat tcgactgcag gaagaagaat ttaaatttat ttcatgtaga
    30481 atcatttttg cgttatatat agatgaaagg gaataaatgt ggttttagac caaattaagg
    30541 ataaaataaa actaaatatc aaaccaagca ggtacataca ctcggaaaat gtaatggaag
    30601 ctgctaaaaa cctggcagga atatatggag cgaatgttga taaggcagct atagcaggca
    30661 ttttacatga ctgcgcaaaa ggaagaacgg caaaggagct attaagtctt tgtgaaaaat
    30721 ttgacatgca tgttgataat attgaaaaaa gacagccgga acttttacat ggacctgttg
    30781 gagcttttat tgcggaaatg gattacggta taaaggatga ggaaatcctt aatgctataa
    30841 gagttcacac tacaggaaag gaagatatga acatactgga gaaaattgtt tttcttgctg
    30901 attacattga accaggaaga aattttccgg gggtttgcga aataagggaa tgggcatgta
    30961 aagatataga tagagcaata ataatagctt tagatactac tattaaatat gtaatagcaa
    31021 tgggaggcct gatacatcct gattctatag gggcccgaaa ctatctgtta ttacagagag
    31081 ctaatataca gaactgattt ccggcagttc tataaaaata cataaatcag aattgtatag
    31141 aattgttaac aaaacaactt aaataatgta taatagagaa taatgtataa taacgaataa
    31201 tgcaattata ttgagtaaag tattatgcaa agcttgcaga aaatacagac taatagcata
    31261 tatataacgc aaaaatgatt ttacaaagaa caaatatgaa caacaatact tggctggagg
    31321 gagagaatat tttggatagt tcggtattag ctgaaaaaat aaccgagata cttctggata
    31381 agaaggcaaa agacgtggat gtaataaaag ttgaaaatat tacaattctt gcagattatt
    31441 ttgtaatatg tacaggtaca tccaccaccc atataaaaac acttgcagat gaagtgattt
    31501 ttaaattgaa ggaagaggga attgagtttc accatatgga gggctatgag agcagccgct
    31561 ggattcttct ggattatgga agcgtagtaa ttcatatttt ccatgaagaa gagagaaaat
    31621 attacagcct tgaaagatta tggtctgatg gcataattac cagaaaataa gtgatatagg
    31681 tatgtaagca aaataattaa tatgagtaac ttaagtaaaa taaataatat aagtatatta
    31741 ttttgtaagt aataaatata tataagagat atgagagata taagcaatat aagctgaagt
    31801 tagctgatag aagggagtga attgcaggtt ctccttgcat gattttcatt aaatcatgag
    31861 cctctgaacc tgcacgatta tgaaaagcta taatgcaaaa gatatcgaaa agaagtggca
    31921 aaaaaaatgg gaagaagaaa atactttcaa ggtagaagag aatggcaagc ccaaatatta
    31981 tgtacttgaa atgtttcctt atccctcagg taatcttcac atgggccatg taagaaatta
    32041 ttctattggg gatgtggttg ccaggtttat aaggatgaat ggctataatg tacttcaccc
    32101 gatgggatgg gatgcttttg gtcgtcctgc ggaaaatgcg gcaataatgc atggaactca
    32161 tccttttgat tggacccggt ccaacattga aattatgcga gggcagttaa aagaactggg
    32221 attaagctat gattgggaca gagaagttac aacctgccac cccaattatt ataaatggac
    32281 tcagtggatg tttctgcaaa tgtataaaaa tgggctggca tataagaaaa aatccaatgt
    32341 taattggtgt ccttcttgtg caacagtgct tgcaaatgaa caggttgtaa atggtaaatg
    32401 tgaacgctgt aaagctgatg tgggaaagaa ggatctggaa cagtggttct ttagaataac
    32461 aaaatatgct caaagactcc tggatgatat aaataaatta aaaggatggc ccgataaagt
    32521 aaaacttatg cagcaaaatt ggataggcag gagcgaaggt gtagaagtag attttaaagt
    32581 agatggcatg gataaagggg taagaattta tactaccaga ccggatacta tttttggcgt
    32641 aacctatatg gtaattgccc cggaacacag aatgacaact gaaataatta aaggtacacc
    32701 acaggaacag acatgccttg agtttataac ccgtatgcag catatgaatg agattaccag
    32761 aacatctgag gagactgaaa aggaaggtgt atttaccggc aggcatgtaa ttaatccttt
    32821 aaatggcgat aaagtacctt tgtatattgg aaactatgta ttggctgaat atggaacagg
    32881 cgttgtaatg gcagtacctg cacatgacga tagagatttc cagttcgcta aaaaatataa
    32941 tctgcctgta aaagttgtaa ttcaaccgga gggtgaagag ttaagtgccg atactatgaa
    33001 aaatgctttt acagaggttg gttatcttgt aaattccggt atttttaaca gcactagaag
    33061 cgatgaagct attggaaaaa ttattgacta tattgaagaa aaagggtatg gagaaagaaa
    33121 ggttaatttc aaattaagag attggctgat ttccaggcaa agatactggg gagctccaat
    33181 accggtaata tactgtgatg attgtggtat tgtaccggta cctgaagaag atctgccagt
    33241 tattttgcca acggatgtta agtttagagg aacgggagaa tctcccctaa tcagttctca
    33301 atccttcttg aaggcaaaat gtcctaagtg tggtaaaatg ggaagaagag aaatggatac
    33361 aatggatacc tttgtatgtt cttcctggta ctatatgcga tactgcgatc cttgtaatga
    33421 aaggctgcct ttcagtaagg aaagcaccga ttactggatg cctgtagacc aatatattgg
    33481 tggtgtagag catgcaatac ttcacctgct ttactcaaga ttttttatga aagcattgca
    33541 tgatatggga tttgtatcat atgatgagcc atttacgaac cttttgaccc agggtatggt
    33601 attgaaagat ggggcaaaga tgtcaaaatc cataggtaat attgtaagtc ctgaagaaat
    33661 tattgaaaaa tatggggctg atacggcaag gttattcatc ctttttgcgg ctcctccgga
    33721 aaaggatctt gaatggagtg aacagggagt agaaggctgc tatcggttta ttaacagagt
    33781 atggagaata gttgatgagt acagtggaag tttgaaggac gaccaagcat gtgcttgcag
    33841 ttcctcatgc ggatgtggct gcggctcttc atcaggagaa aaaataaccg gagctgacag
    33901 agagttaaga tatattgtaa actatgctat taagaaggtt acagaagaca tccgtgaaag
    33961 attcaatttc aacactgcca taagtgctgt tatggagctt gtaaatgctt tatatgcata
    34021 taaagagaaa gtagctgaaa ataagcggaa tccattcgta ataaaggaag ccattgatag
    34081 tcttattgta atgctggcac catttatacc ctatgtaact gaagagttat ggcagtcaat
    34141 cggcggagaa ggaagtgtcc acgaacagat atggccgtca tacgatcctg aagctcttgt
    34201 aaaagatgaa gttgaaattg ttgttcagat aaatggaaga atcaaagaga agattatgat
    34261 acctacaggg cttgataaag ataatacaga aaaggaagcc cttaatactg aaaaagtaaa
    34321 gcagttgata gaagacaaag cagtagtaaa agtaattgtt gttcctgaaa agttggtaaa
    34381 tattgtagtg aagtaaagta ccaaccaacc tgaaggttaa gagcttgcaa aggtacaaaa
    34441 ccgttgatta gtttaagtgt taagttaagg tattcattcg ccagccttaa tgaatggtga
    34501 tgaatacctt aattttgtaa atataaatat attcatttca tattaaccaa gcaggaacaa
    34561 aatagttgtc tttatctatt ggaataatat cactacaaag acatataaca tttcctttac
    34621 ctatttgctt tcctgttttt tgcagtactt caaaatgctt tatcgcatct tttttaggac
    34681 ttccgctttt tttaatttca ataggataaa gagtgttgtc ctgttcaata attaagtcaa
    34741 tttccttatt gtcagaatct ctgtaataaa aaataggtgc tcttttacct gcattgtagt
    34801 aactttttat aatttcgctt acaacatatg tttcaaaaaa tgcaccagac attgctgata
    34861 cttcaagcgt ttcgctactg gtccaacgag tcagatatgc gcaaagccca gtgtccagaa
    34921 agtacatatt tggagacttg attaaacgtt ttaatgaatt attgaaatag ggctctacta
    34981 gtgtaattac acccgatgat actaaaatag acaaccactg cttagcagta ggaggactaa
    35041 tacccacatc ctttgccata tccgcataat taaccatctg actggttctt gcagcgcaac
    35101 aggtcataaa tctcaaaaat gccagttcat cacctacctg cgtcaagtcc cttatatccc
    35161 gttgtaagta agtactaaca taagatgaat aaaacatttc tgtatcttgt tctgtctcat
    35221 acaaagctgg catagaaccc ttgaatattc gcgaaaatat ctcccttaaa ttctgcggcc
    35281 ttacataatt aactttctct aataaatcct taatattagt tttatatggg ttgccctggt
    35341 atccttgtat ttcactggta gacaaaccta gcatattaat cactccaaca cgcccggcaa
    35401 ggctttcact aacgtttctc atcatgtgaa acacctgtga acctgtcaac cagaagtcac
    35461 ctttattctt atgctgatca acatacattt taatatacgg cagtaactgg ggagcatatt
    35521 gaatttcatc aataataaca ggcggttggt acctttgcat aaaaagagcg gggtcactta
    35581 cagccagttc ccttacaata ggattatcca gagttatgta tttacggttt tcctcagcca
    35641 ttttgctcaa gagtgtagtt ttacccacct gacgaggccc tgttaccagg acaacaggga
    35701 agttaatgct tgcttttaat atcacttttt ccaaatgtct gtctatatac atatagtacc
    35761 tccggtaaat ataaaataca attttattat actggaactt agtttattta gcaagatatt
    35821 atgtttattt ccttatttcc actattttga tctatattta atcatgcgtc tcttaatatt
    35881 aataattctg gatattcttc taaggctgaa tctcaaagct ccaaaagaaa ataatataat
    35941 aaaaatgctc tttgctattt tatataaagc actattttta atgcttcctg attttccact
    36001 gaaagctctg gctatattac cggcgagctt ttgaatagcc cttataaatt taggtttatt
    36061 agatttttct actgaagttg atgtaataag gttggttttg cctaataaaa tatcgccctt
    36121 ccaaaattcc atgtatccta gaacttcacc ctcatttata ggagctgaaa tagttggagt
    36181 cacatgaact tttttaataa cagaccaatt attggacatt gcatttgcac ttttgtcttc
    36241 acctgcaagc acatcattgc tggcatctgt atctttatct ttacctacat atgcatatgc
    36301 atatgcaggt gtcccataac taagatttgc aggaaatacg ctggaaacat cggtttcagc
    36361 tatgaggttc aaaacagctt tatcctttgc atctttcaca ctcacgttgc ccgccggtga
    36421 gttttgtttt ataagtgtac gaagggagta attttcaaac ccatatttta gaagcgtttc
    36481 agtatattta tatatgtttt ccggtgcccg ttcattttta acacctaaaa tgacagatat
    36541 aagctccatt ccttcctcat taactgctgc tgatacaaga ttgtgacccg caggtgtagt
    36601 aaaccctgtc tttatgccaa taatattaaa gttgcagtta gttttagtac cgtatcgcaa
    36661 taatttattc gtagtatata aagtgttcca ggttgaatgt ttattggttg aaggcatggt
    36721 ataactatcc tttttcacaa tttctctgaa cgctggtaga gacatggcat gccttgcaat
    36781 taaagccata tctcgtgcag tagtataatg atccatgtga tgcattccat ttgtattgat
    36841 aaaatgggtg tttactgctc caagttcctt tgctcttttg ttcataaggt caataaattc
    36901 atttctggat gatgctgtgt tttccgcaat aatatttgca gcttcgttgg ctgaacaaac
    36961 aagcatagca ttcaaaagat cccacagggt taattcctcc cctggcatta tacctatatt
    37021 cataccccca ggaccaatat catcaattgc agcctggctt gctgtcatta ctttatttat
    37081 gtcttcattt tcaagagcta ttattgcagt cataattttt gtggtgcttg cagggtatat
    37141 ttcagttttg tctgcatttt cactgtaaag tacaagaccg gtccttgaat ctatcaagat
    37201 atatccctca gcttcaatat ttagctctgg caactctaaa tttgctgcat aagaaacgga
    37261 atccgccgca ggtacaatcg gagaatttag actagaggaa ttatcagcat aaactatgcc
    37321 tgaaaaagca gtaaaaatta aagataatac taatataatg tataaaaaag acaaagatac
    37381 attgctaatt aaatatttcc taaaaaccca acatgtacca taacctgact ttttatactt
    37441 agtggactgt atatttccca tttttttctt ttgctacctc caaaaaggat aatcaaatac
    37501 ctgattttta gtataccaaa aattacagtt taaataaata aaaaaatttg atagaaaaaa
    37561 aggaaaaaac aaacacgtgt agaataatat atacaaagga ggtaaattat ggaactaagg
    37621 atgtttaata aaaggttata tattaaaaaa gaatatgtat cgatatcaat tatttttgtt
    37681 attatggtaa ttatattaat aatatatata tccgtaaaaa acaataatga agttgttatt
    37741 gatagttgta aatctccagt cagagattct gccaaagatt cagataatga ccctgttaat
    37801 aataatccag ttaaagatta ccctgaagcc aaccctaaag ctaaccctaa agccgatcct
    37861 aaagccgatc ctaaagccga tcctaaagcc gatcctaaag ccgatactaa agctgatcct
    37921 gaagctgatc ctaaggctga tcctagagct gctcctgaag ccgatccaaa agctgatcct
    37981 gaagctgatc caaaagctga tcctgaagct ggtgagacaa ccctggaaat taaggtatat
    38041 ataattggtt gtgtgaataa tcctggtgtt attactttaa agaaaggtca gatgatatat
    38101 gaagctatag aaatagctgg cggggcgaca gccgaagctg acctggaaaa cataaacatg
    38161 gtttatattt tggaggaaaa tataatgatt aatatactgg cacgagataa agatagaaaa
    38221 cttattacat ctgattctga agcaggaaat aatggaatac ctgatattaa tatagccgga
    38281 agcggaatac aaataatcag agataccggg gacgcagtta ttaaaacagg tggtaaaagt
    38341 actgataata atatgggaca aactgatggt acaggaaaaa gcagtgattc tggtaatatt
    38401 aataactcaa aaggcaaaat aaatattaat aaagcaagtg taaatgaact tgatacatta
    38461 cctggtgtag gaaaagcaac agctgaagat attataggat acagagaaaa gtatggcagg
    38521 tttgccaaaa tagaagacat aatgaaagta ccaggtataa aagaaaaaaa gtttgagaaa
    38581 atcaaagatt ttataactgc tgactagatg attgaaaaat gtagcattgc tttggggttc
    38641 acttaaaatc aaaaaaggac tggtaatata accagtccct tttaatatat ataaaaaaag
    38701 gaggaaactt catgtttatt gcatgtttat tgcatgtttc tttatctgat tatattatag
    38761 atgtttattg aaaaaaaaga aatgctattt attaacgaca ttttccaata tatattatga
    38821 atttttatgc aatattaaca acagaaatta ctgggatatc aatggcaaaa taaaaagatt
    38881 ggtcagtata taacctaaaa atacagatgt agaagggata acaattgtat atagcagaaa
    38941 cctcataatt cttctgcgcc tgtattttct gaacctttgt actcgatttg ttctcatgaa
    39001 attcacctcc caatatataa caggtacttt gtcataagtt acgtctatat tgtaccataa
    39061 acaaaagtaa tgttacataa tgtggaaata tttcctttca tcttcccgat gctattaaaa
    39121 attatcacat aagacatcgc tattcaaatc ggcatagtag tactgaaacg aaaaattcat
    39181 aataatatgg atattattga aaatgcatac taaagtcata attgcatatt gctttatagc
    39241 tggcccaaat gctaatattc agtttggatg aactcattta tataaactat tttttatgca
    39301 aaatgaatct gcataatata taagatgaat cgatgaatct aagtaaaaaa gttaagttat
    39361 atacttacat aaatttatat ttagagttat ttattgtttt aaggaggtca cctatgaaat
    39421 ttcctgttaa aaaagccggt aatatactta tggcatttat gttattaatt caattgatca
    39481 tgtcacctat aaaagttttg gctgaggaac ctcttagatc aaaattcaat tatggaaatg
    39541 aaaatacttc agttgaatat taccgtaaag taagtgaacc atattatgca gcactgctta
    39601 aagaatatgg ggaaaagagt tataagggcg ctaatattcc ggaaatctta gttgagcctg
    39661 cagatattgt cttgccggag ggcaaagaat taaagcttga atcaaaactg ggcgtaagtg
    39721 attatcctgc atttaaatgg ttgtgtgata taccctgggc tgaatggaac tttgaaataa
    39781 gcagtgaggg actgtatgaa attgaactgg aatattatat gctgccgggg acaggtaatc
    39841 cggctacacg ctcacttaag atggatggta ttataccatt tttcgaagca tcaaatatag
    39901 ttttttacag gggctggaaa gacgaaagtg aaccaattgt taatagtatt ggtgatgaag
    39961 tgaggccaag ccaggttgaa attcccggct ggagggtaat gggactttgt gatgcgcaag
    40021 gctactactc tcaacccttt cgtttttatt taaagcctgg gaagcacaca ttgcgtatgg
    40081 aatatgtaga ccaggacatg gcaattggca ggatgcgggt aaggcctgtg aaagaattgc
    40141 ccgcttatga acaagtgaag aaagaatata agaataaagg atacgcaaac atagatgaaa
    40201 cagtagaaac agtagaatta caggctgaga gtacagcaac cgaaaagagt gaccctacaa
    40261 tcagaagaga aagaaacggt gatcctgttt gcaaaccaga atcttctcta tacagaaaat
    40321 taaatgtttt gggaggttat cgctggcgta agggaaatca gagtataaca tgggaatttg
    40381 atgctcctca ggatggttta tataaaatag cagtaagagc attacaatca tggaatgatg
    40441 gtttaccttc ttacagacaa atagacatag atggagaaat accttttaaa gagctggaag
    40501 aatacaaatt tgtttccaat agaaaatggc gtaccgaagt tctggacgat gagaaagggg
    40561 aaccattcct tttctactta accaaaggaa cccatactat atcaatgaca gttaaaatgg
    40621 gtgcacttac tcccattatt ttatcgctaa atgatgatgt tatattgctt tcaaatttaa
    40681 taagagatat taccaaagta acaggtagta atcccgatcc aaactatgat tatgactttt
    40741 tcagaaccat acctcactta aaaggtaata tggagatttt aatggaaagt ctgcagtcaa
    40801 aatacgatat gcttgaggat atatcagaga agctgcctgc aatggcaaac aacctgatga
    40861 ctataaagag ccagcttgca ggtatggtaa aagacccctt tagtattgca agaagattaa
    40921 atgacttaaa caatgcttta tcaaaccttg ggaactggta tttgagtttg caaaatcaac
    40981 ctttaatgat tgattatttt ttagttggag ctcccggtgt agattgggga aatgaacagt
    41041 ctaacgtttt ccaaaaaata aaggccactt tagccaactt tattgtttcc ttcaaaaaag
    41101 actacaataa tataggcagt gttgtcaaag acgatgtaga tataaaaaat acaattaacc
    41161 tttgggtttc caggggtaca gaatgggctg aactaataaa ggaaatggta gatgaaaagt
    41221 ttacacctga gacaggcatc ctggtaaata tcaatgtttt tcctgcagga caattacatg
    41281 caggttctgt taatgctcta atgcttgcaa ttgcttcagg cagggcaccg gacggtgcat
    41341 taggaattga ttcaggttct cctgttgagt ttgctataag agatgcagta tatgatctaa
    41401 gcaaatttaa ggatttccag gaagtatcaa aacgcttttt gccaagtgtt tttacaccat
    41461 ttaaatataa cgacgggata tttgcacttc ctgaaactat gaatttcatt gcgatgatct
    41521 atcgtaaaga tataatcagt gaatatggaa taagacttcc aaacacaagg gatgagctat
    41581 ataacaatgt attgcccatg ctttatcaaa acggactgca gtttaattat cctgctgatt
    41641 tcagccagtt catttttcag catggtgcgg aatattatct tgataatgga gcaagatcag
    41701 gtcttgaaac acctgaatca tacagagctt tcaaagaagt aacagaacta tatactcatt
    41761 acggaatacc ggtatcagcc aatttcttca atcgtatgcg cacaggtgag atgcccatag
    41821 gaattggaga ttacaatctt tacatacagc tttcagtagc agcacctgaa cttgcaggaa
    41881 ggtggggcat tgctcccata cccggtacct tgcgctcgga tggtacaata gaccgctcca
    41941 atggaagtat aacaggtcag gcaggtgtca tacttaatca gtcgaagaag aaggaagaga
    42001 cttggaaatt cttaaaatgg tggaccagca catcagttca gaccaaattt gcaagagagc
    42061 tcgaagcaat aattggtact gaagcccgct ggaatactgc caaccttgaa gccttcacaa
    42121 atttggcctg gaagaaggaa gacttggaag taattaaaga acagtggcaa tgggctaaag
    42181 gagttccggt agtcttgggg ggctactata ccggacggta tttaagcaat gcatggaata
    42241 gtgtagtaat aagcggtgtt cctgtaagag atgcacttga atctgcagta gaggaaataa
    42301 atcgtgagct aagaatgaaa agggaagaac atggcatatt tgataatcag acacaggggg
    42361 tgggaaaatg agagcaaata ctaaagtcga gactaagatt aagacagaag ctgagattgg
    42421 tagtgcaaaa ataggagtaa tagaagtcaa tgccagggca aaaatggcag atggaaccgg
    42481 aacaaagcct aaaataaagc ctaaaataaa gccatcttgg aggaaaagcc ttataggtta
    42541 tgtatttatg ctgccttttc tcatcctatt tttcatattt attataatac ctgtatttgt
    42601 tgctctagga acaagcttta caaactttaa catgctccag cctgccaaat ggataggttt
    42661 gacaaattac aagctgcttt tcttagatga cgtcatattc cttacagcaa taaaaaacac
    42721 tttgatattt gcctgtatta caggtcccat aggatacttt atgtccttca ttgcagcctg
    42781 ggtaataaac cagctgcgta tgcgcaatgc ctttgccctt gctttttatg caccgtctat
    42841 aacaagcggt attgcaatgt cggttatctg gctgtatttc ttttcaaatg accgatatgg
    42901 cttgtttaac aacatattta ttaatcttgg atttataaaa gaaccaatat tatggaccct
    42961 tgatgcaaag tatattcttc ccgtaattat attgatttcc atatggatga gcatgggtac
    43021 agggttttta gtatttcttg cgggcttaca aaacattccc agagaatact atgaagcagc
    43081 ctctatcgat ggtataaaaa acaagttcca ggagctttgg cttataagct tgcccctcct
    43141 aaaaccccag ctattatttg gtgcaataaa ctcaatagta gggtcttttg gagtatttga
    43201 catagcagta tcagtagcag gtatgccaag cccaaactat gcagctcaca ccattgtggc
    43261 acatctttac gactatgcat ttataaggtt ccaaatgggt tatgcatctt cggtagccat
    43321 ggtactattc ctaatgacat tcatattggg acgtgtggtt atgaagatgc tttcatccaa
    43381 aggagaatag aagtgaaaat tgagaattaa gaaactggtt atgggagtac aaagctacaa
    43441 gtcgaacagt tgctaccagg gatcaagaac cgtatagact cacttcccaa ctcttacctc
    43501 ttaattctaa atcaatacat aatgacatgg aggaataata tggaagtaaa tgtagtaaaa
    43561 cgatttgtta taaatacata tagaaaacta caaaaaagac gggcagcaaa aactgcatat
    43621 acaagaagat atgaaaaaac tactgcatgg gatgtgcttc ttcatatttt tatggtggct
    43681 cttgtagctt ttacagcctt gccgctggtt tatatgatta gtactgcgtt taaaccactg
    43741 gatgaactat tcatattccc accccggtat tttgttagaa agcctacgct tcaaaacttt
    43801 tttgatcttg caatatctct tgaaagttca acagttcctt ttatacgcta catatataac
    43861 agtttggttg taacaactat taccgtaaca tgtactgtta tagtatcagc cttgggagca
    43921 tatgggcttg tcaaacacaa accaatagga cataaagtag tatttaattt tattatagct
    43981 gcccttatgt tttcacccca tgtaactcag atacctcgtt acatggttgt taacaagctg
    44041 ggacttttaa atagttattg ggctttaatt ataccaagta ttgcagttgc atacaacttc
    44101 ttcctgatga agcagtttac cgagcaattc cccgacgagc tcctggactc tgcaagagtt
    44161 gatggcgcca atgaatggac aacattctgg aaaattgtta tgccttctct cacaccggca
    44221 tggtctactt taatagtatt tagctttgta agcaactgga acgattattt ttcaccgctt
    44281 atatatatta ccgataatgc aaaaaagact cttcctccgg cgttgcaaac catttccgga
    44341 ggaggaagca ttggcagagc aggtgctgta gcagcagcta caatgcttat gacgttgcct
    44401 acaataatta tatttacaac aatgcaggcc agggttatgg aaacaatgac tcactcagga
    44461 ataaaaggat aaaggaggga agcagaatga ataaatttaa tagagctata tccttcttta
    44521 tagtaatact aattgtattt tcactatgga ctaatatagc acaagctgca agccgcgatt
    44581 atataatcga tgatggcaga aggataccaa tacctgaaac atacatatta aaagatataa
    44641 tatataatgt aaaaaatcat ggggatgaaa ttaaatacta tgaagcacca accgaccttt
    44701 ttataaatga acaggattac ttatttatag cagatacagg aaataaccga attgtcaaaa
    44761 caaataaaag cggtgaaatg atagcagttt ttaaagaagc caatggaaag gctttcaact
    44821 caccaaaagg tgtgtttgct gatgcacagg gaaaccttta tatagcagat acaggaaatc
    44881 agcgaattgt gcatctctct gatacaggtg aattcgtaga agaatttgta aggccgaaat
    44941 cagatttgct aagtgaaacg ttcacatttg acccaacaaa attatgcgtt tctcctacag
    45001 gttatatata tgttttaaaa ggagaaaaca tattaattct ggatgcatat aacaacttca
    45061 ggggatatat gggacaaaca gatatcggat tcaggctcct tgatgttctg cttcgtatat
    45121 ttgcttctga agagcaaaaa cgtattattc gtaaacgaac agcagcttca tacctaaata
    45181 tatctatgaa tgataaaggc atgatatatg cagtaactat ggattatgca acaggagaaa
    45241 taaagaagct taattcagtg ggcagtaata tttataggaa atataaaaca ctgcaaaaag
    45301 gtgcaaattt acttaatctt gatttcctga ctacaatgaa gctggaaagc aggagctttg
    45361 tctttggaga gagaaatgat ggagaaggaa gggttattat gcctttgttt aaagacattg
    45421 ctgtagataa gaatggaata gttactgttg tagaagatgt aaccgggaaa atatatcagt
    45481 atgatcaaga aggaaatctt ctggcagcat ttggaggcaa aagtgatcaa cagggaagat
    45541 tttctgcgcc ggaggctata gaggtagatg ataatggctg catatatgtt cttgacagag
    45601 ttttaggaaa cattcaagtg tttgagccta ctaattttat aaagatggtt cataatgcag
    45661 taaatttata tgcagaagga gagtataatg gagcatataa ccaatggcaa aaagttttag
    45721 gaatccattc aaattatcct ttggctcatc taggtttggc aaaatcgcta tttaaacaaa
    45781 aaatgtggaa agagtcaatg gaggaatata aacaagcaga agatcgtgac gggtattcaa
    45841 aagcgttcat acagtataga tatgaagtag tacgcaataa atttccaata gtcctcctag
    45901 ttctaataat tattataact atatttgttt tattattaag acttattggt aaactggcag
    45961 cagaaggaat agaatgcttc tacatgaatg gtggaaaaag gtttaccatt attgaacaat
    46021 taaaattatc tcttggagtt attcttcacc ctcgagaaac atttgagctt ataaaagata
    46081 aacgtgatag catgagcata gctccgggag ttataattct aggagtgcta tttttggtaa
    46141 gaatatttta tatttttacc gttcattttc cccttgcgga tattgatata agagattcaa
    46201 atatcgtact tgaggcagtt aaactgcttc taccagtagt tacgtgggta attgcaagtt
    46261 ttgctataac ggcaatacta gaaggtgaat caaaattgaa ggatatattt attgcatctt
    46321 cattttccat ggtaccgtat attttagtta cttttccaat tgcaatactg tccaatgtac
    46381 tggcaagaag cgaattaact ttatatgctg ttataataaa tggttcgtgg atatggatat
    46441 tcatactttt ccttatacaa gccaagacat tgaatgacta taaattcggg aaaactattt
    46501 cagtcagtat gataagtgta ctaaatatgg ctttaatatg ggcagtcgtg cttatggcat
    46561 atgtattgac cggccgctta ttccaattcg ttgcgggaat attcagagaa attagaatga
    46621 cttttaactg aaagggggca aagaagatta tatgataaag aaaacaaaaa gtttattact
    46681 tataaaaaag tttaaaagtg ctataaaaga aaataaagtt aatagaaata catattttaa
    46741 aagtaaaata tatagtattg ctgctttaat tactgcgatg atagtcgttt cagttttttg
    46801 ggggataaat gttacagctg aaccggctaa ttctgaaaat accaggcatg ataatactgg
    46861 gattgaagta gtcagccagt caaaaaagga tgaaaatagt attcatcaaa ctgaaataaa
    46921 taaatctaaa ctaacgtata ctactgtacc tgatggttat aaaataattg agcagaattc
    46981 caaatttgcg ctttattgca acactaaaac aggagcaatt attgtagaaa acagtgaaac
    47041 aggataccta tggagaacaa tcgtagacta tccggaagat tatgcgctcg aaaatctaag
    47101 tgcccactgg aaaagctata tttcatcact gtttactatt agttacacag atttgcagaa
    47161 aaatgaaggc cttatggaaa aggcattttc atctactgat gcaactataa ccaattccag
    47221 tattattaaa gatggtataa gtttagatta tgatttcaat gaaataggta ttaatataac
    47281 tattgatatt attttgcagg aagatggact tattatttca attccaagtg agaaaattaa
    47341 ggaaaacaaa aattttggat tagtaaacat agaactagcc ccataccttg gtactgagaa
    47401 cgataattta gatggttaca tattttatcc tgatggatgt ggagccataa tgaaatacga
    47461 gaaatcgaaa gaacgcccga aatttattaa gccttacaaa ggattcatat tttcaccgga
    47521 aaaagtggat atttccgaat atataaccat ggaaagaaac caaaaataca atgttatgct
    47581 gcctgtttac ggaataagaa atggtgatga tgcttttctg gctattgcat caaagggtgc
    47641 tgaagaaaca gctatacttg tatatcctta cggttattcc ataaacttaa acagagctgg
    47701 ttttgaattt tattataggc atttttacaa caccattctt tctaatatta cactccgagg
    47761 tagagatacc gcaaagaata tttcaggatt aaaggctgac aaagaactta ttatgcagga
    47821 tcatgaggtt acaattgttt ttctagacaa atccgatgcc aactatagtg gaatggctaa
    47881 tgcttatagg gatcatctga tgaaaaacaa tgaactgaaa agggtcataa aaaatacaga
    47941 cagtatacct ttagaaatta gtttatttat gggaataaaa gaagaaagaa tgctatttga
    48001 taagtatatt tccatgacaa gctttcagca ggccaaaatg attgcagata agtttaagga
    48061 taatggtatt gacgctttac agataagtct tattggctgg tcgaaaggtg gatatgggat
    48121 ctatcctgat atttggcctc cggaacgcaa actcggcgga gctttagaaa tgaagaaatt
    48181 gttagattat acatctgaaa atagtataca agtgtttcta cgaactaatg ctattgatgc
    48241 tattgctgat aataagactt tttccaaacg aaatgatgtt gtcattcaag gaaatggcct
    48301 tccggcaaca ggtttcaata aggatagatt tattttaagc ccttttgctt attttgaaag
    48361 gattaagaag cttataaaca taatacctgt ggaagatggg acaggtctgg ctttggaaaa
    48421 aacaggtaaa atgctttacc acgactataa caaaaataat ccttcaagca gaacacagac
    48481 aatgaatacc tggggagcaa ctcttctttt agctaataat caaaataaac ctgtgggtgt
    48541 tgaaggaggt aatgtttacg cattaaaata tgcggattat ttattcaata ttcctgtaaa
    48601 atcttctggt taccatataa cagacgaaac aatacctttc tttcaaatgg tggtacatgg
    48661 aatgatacct tatacttcag aaccgggaaa tttggcatat gatcttacaa tacagaaact
    48721 taaatggata gaatatggct gtatgccttt ctttgaactt tcttatgaag acactacaaa
    48781 actaaaatat tctagataca acaggttatt taactcccaa tatgaatact ggcttaatac
    48841 tgctgtagat atctataaag aattcaatga tagattacag gaaacctgga atacagaaat
    48901 gataaaacac gaaaaattga acgccaattt agtaaaaatt agttacagta atggcgcagt
    48961 tttatatatt aattattctg acgaagatat tattcaggat ggctatagga ttggagctaa
    49021 agattatata gtaatagaca gaaagggggc tgttcgatga aaaagagttt taaaattacc
    49081 ggagaaaaaa ggcatcggtt ggaaggccta atatttgtgt caccttttat tataggaact
    49141 cttatttttt tcatatttcc cttatttgta tcaataaaac ttagttttgg taaacttaag
    49201 aaaatggaag gatttgttat tgaatggctt ggacttgaca attatataag ggcattcata
    49261 ttagatacaa gttttatacc aatgtttttg caggtaataa gaaagacaat cgttcaagta
    49321 ccccttatta tcgttttctc aatattgctt gccatgctaa ttaataagaa tattaagttt
    49381 aaagggcttt ttagaacaat attctttata ccatttcttt taggaatggg agatgttatg
    49441 aatcagcttc taggccttgg aatagataag catgtgtttt catttgttga gagcaatttg
    49501 ttaccaagag aattccttgt atatttaggt ccaaaagtgg tagaagcagt agaaagcttt
    49561 tttgggataa ttgttgtagt tctttggagc tccggagtac aaatactgtt gtttttatca
    49621 ggacttcaga gtattccagt ttcactatat gagtctgcca aggtggacgg agcaactgaa
    49681 tgggaaatgt tctggaagat tacgcttcct atgatttcac caataatgct gttaactata
    49741 gtatatacaa taatagcatc atttacagat atatcaaacc ccatattagc atatattcaa
    49801 tcaaatgctt ttcgttccct gagatttgaa tatgcggcag caataggctg ggtttatttc
    49861 ttgtttatta ttttgctggt atcacttgtt tttatactgc ttggaaacca tatttatact
    49921 acgtctgaat tgaagggggg agcgaagaat gagcgaaaag attattaata atataaagtc
    49981 aaaattagta ataaaacgaa caggtaatat aaagcataat gtttttagag gagctactta
    50041 tcttttaatg ctggacatgg catttgtatt cttgttccca tttttgtata tggtggtaac
    50101 atcaattaaa agcaataaag atttgtatga ttttacagta aactgggttc caagaactat
    50161 tgaatttaaa aattatatat tagcttataa agcattagaa tatcctagat tttttaagaa
    50221 ctcttttatc atgactactg taacaacaat agggcatctt ttatcttgct cttttattgg
    50281 atatggtttt gcgagatata attttagcgg t
//
LOCUS       DAOSDD010000004        47271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_440_length_47271_cov_4.981616, whole genome shotgun sequence.
ACCESSION   DAOSDD010000004 DAOSDD010000000
VERSION     DAOSDD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 47271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47271
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_440_length_47271_cov_4.981616"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PK733_00600"
     CDS             complement(<1..358)
                     /locus_tag="PK733_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ09077.1"
                     /translation="MQNVKPPNILLILADQHRYDCIGYSNEYPIHTPNIDKLACEGMW
                     FTNAYTPIPVCCPARQSLLNGRRPETFGALWNYNNGLKVSTLQPTDYTWVRGLKEHGY
                     LTGHIGKWSISPEYNPN"
     gene            complement(511..1323)
                     /locus_tag="PK733_00605"
     CDS             complement(511..1323)
                     /locus_tag="PK733_00605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ09078.1"
                     /translation="MAYKLLAVDIDGTLLDSKGNLTHETIKAINLGIEKGMIFTISTG
                     RPIQGLKSLIEKLELTHDLPFITYNGAMVIMWKSKDILFECSLNNEDAMNIIELGKKL
                     GTTVMVWTDNKLYASELSDKAYKYSSISTVTPLLLGNDRDSIEKTIAKGVTKILWYDE
                     VETINRYLNEVSGYLSENVNFHTSQPYFLEFVDKRASKAKAMEKLGEHFNINIREMIA
                     VGDGLNDLSMIEYAGLGVAMENANDAVKEKADYITLSNDEDGVAHVIYKYIL"
     gene            complement(1478..3538)
                     /gene="htpG"
                     /locus_tag="PK733_00610"
     CDS             complement(1478..3538)
                     /gene="htpG"
                     /locus_tag="PK733_00610"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOJ09079.1"
                     /translation="MAREVGNLSIHTENILPIIKRWLYSDKDIFAREMVSNGTDAINK
                     FKRLVSIGEAKADDDEQFRITVIVDKDKKTIQFIDNGIGMTDEEVKKYINQIAFSGAT
                     DFIEKYKDKMDQDNQIIGHFGLGFYSAFMVSEKVQIDTLSCQEGAQAVRWTSTGNDQF
                     EMDESDRRERGTTITLFVSEDGQEYLDEYKMREVLTKYCAFMPYEIYLENAEDKKKKD
                     TKDKIIESEKQDGKLKDSDTNVEETSLDAEETGKDAKDADTADIKDDIKDDIKDDMKD
                     EDDDDSPKPINDTQPLWLKKPKDCTDEEYKEFYKKVFHDFNDPLFWIHLNMDYPFNLK
                     GILYFPKLKHEFETIEGQIKLYYNQVFVADNIKEVIPEFLMLLKGTIDCPDLPLNVSR
                     SFLQNDGYVSKISNHITRKVADKLVSLSENERDNYNKYWDDINPFVKYGCIREQKFYD
                     RVKDIVVYKTTKGDYITLKDYLSRNESKHKNKVFYVTDEKQQAQYIKIFEENELEAVI
                     LNTLIDSHFIQFMEMHESGVTFSRIDSDISESLKAGDGKENDSPAEKENREALEKTFK
                     EAVSDEKLKVQVESLKTASVPGMILLSEQSRRMQDFSRYFGGQDMSHMFPKEETLVLN
                     DKNRLIQLILEMKDKEDKKDDVNLLCRQVYDLAMMSHKQLEPEAMTKFIERSNIILEK
                     MAEK"
     gene            complement(3770..4621)
                     /locus_tag="PK733_00615"
     CDS             complement(3770..4621)
                     /locus_tag="PK733_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ09080.1"
                     /translation="MAEIFQYEFMRNAFIVGLLISIIIPCIGVVVVLKRLSMMGDTLS
                     HISLAGVAAGLVAGVNPVAGAVTFSILASFGIEKIRKSFSQYAEISLAIIMAAGIGLA
                     GILSGFVKNSSAFTSFLFGSIVAVSRFELLTVIILSVVVLFAFIFMYRDLFCITFDEE
                     SAKLAGIAVARVNFIFMLLTAIVISISARTIGTLVVSSLMIVPVASSMLISKSYKETV
                     IYSIVFAVISTISGLIISFYADVKPGATIVLTGVIILLLVMLIKKVFYTLHIRRDISK
                     QHNSQLE"
     gene            complement(4659..5471)
                     /locus_tag="PK733_00620"
     CDS             complement(4659..5471)
                     /locus_tag="PK733_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOJ09081.1"
                     /translation="MDKALDVRNLSFSYNGKTVFDNVSFDICRGDYFGIIGPNGSGKS
                     TLVKLFLNFLSPNGGNINIFGYNVKNFPDWSKVGYISQRAISFNMSFPATVEEIVGSN
                     IYRKAKISGLFTANLKKQRKEKVYNALKIVEMEDNAKKLIGNLSGGQQQRVFIARMLV
                     NNPEIVFLDEPTTGIDAKSEEAVYCLLAKLNKELGITIVMVTHDIGAITVHANKIGFA
                     GNKDVKIISPKDEITEDLIDELYGYKINIHLNRHECTTCYRNAKLKNKTGGI"
     gene            complement(5721..6728)
                     /locus_tag="PK733_00625"
     CDS             complement(5721..6728)
                     /locus_tag="PK733_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOJ09082.1"
                     /translation="MKSNVKTYKVTIIILLPIFLMTLICATIFTGCTGRKSVEGQAGT
                     ENKISVTASFYVVYDFAGKIGGDKVTVKNLLPAGSDPHGWEPSPKDIISIEKADVFVY
                     NGAGMEGWVDKVLESISNKNLIIVEAAKDIELRKANHEHESEHGHDEKDGEKQADQEK
                     KHIYDPHVWLDPAMAKIQMRAIADALVKVDPDNKDYYEKNFEKYSRELEKLDSEFREA
                     ASGFKKKDIVVSHEAFGYLAAAYGLNQVAISGLDAEAEPTAARMVDVANFTKDNNVSV
                     IFFDKLVNPKVANAIAQSAGAKVEVLNPIASLSEKELKEGKDYFSAMRENLEALKRAL
                     K"
     gene            complement(7078..7821)
                     /gene="pflA"
                     /locus_tag="PK733_00630"
     CDS             complement(7078..7821)
                     /gene="pflA"
                     /locus_tag="PK733_00630"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOJ09083.1"
                     /translation="MEIKGRIHSFETFGTLDGPGIRFIVFMQGCPLRCIYCHNRDTWD
                     VSGGREYSVDDVVAQTEKYIDFMQSSGGGITVTGGEPLIQVDFVTALFKRCRELNIHT
                     ALDTSGFAEVEKVMELLEYTDLILLDIKHASEAKHREITGVGNDKIKKFALYTMKKGI
                     PLWIRYVLVPGYTDNEEDLRLAGEFIESLKNVEKVEVLPYHCMGAFKWDKLGLEYPAA
                     NVREPSKEEVARAAETLTPKKLKLLVHSI"
     gene            complement(7847..10075)
                     /gene="pflB"
                     /locus_tag="PK733_00635"
     CDS             complement(7847..10075)
                     /gene="pflB"
                     /locus_tag="PK733_00635"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOJ09084.1"
                     /translation="MEQWKDFKKGNWCSEIDLRDFIQLNYTPYEGDRSFLKGASERTR
                     KLWNKVSELLKKEREKDGVLDIDTHTVSTIVSHKPGYIDKDLEVIAGLQTDEPLKRAI
                     MPFGGIRMVAKGSEAYGKKVDSDIIKVFTEYRKTHNQGVYDVYTAEMRRARKAGIITG
                     LPDAYGRGRIIGDYRRVPLYGVDRLIEEKRNELVHLEFDYIDADTTRDREEIQEQIKA
                     LGFLKEMASSYGFDISKPAGNSREAVQWLYFAYLGAVKEQNGAAMSLGRVSTFLDIYF
                     ERDLREGTITEDEVQELMDQFVIKLRIVRFLRTPDYEALFSGDPTWVTESIGGMGLDG
                     RTLVTKSSFRILNTLYNLGPAPEPNMTVLWSVHLPCEFKKFCAEVSVHTSSIQYENDD
                     LMRTYWGDDYGIACCVSAMKLGKQMQFFGARCNLAKALLYAINGGRDEMTGAQIAPRF
                     EAVGEGYLDYEDVMNRFDMVLDWVARLYINTLNLIHYMHDKYSYESLQMALHDKDIYR
                     TMACGIAGLSVVVDSLSAIKYAKVKPVRDENNTVVGYLVEGNYPKFGNNDERVDSIAI
                     MVVKLFMDKLRKQRTYRNSVPALSILTITSNVVYGEKTGNTPDGRKKGEPFAPGANPM
                     HGRDTNGALAVISSVAKLPYRHAQDGISFTFSMAPKALGRDEEARLNNLMALLDGYFK
                     KGGHHMNVNVFNRETLLDAMEHPEKYPQLTIRVSGYAVNFIKLTKAQQLDVINRTIHE
                     RV"
     gene            10639..11241
                     /locus_tag="PK733_00640"
     CDS             10639..11241
                     /locus_tag="PK733_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ09085.1"
                     /translation="MKELLELFVIFCRIGGFTFGGGYAMLPIIQKEIVEKRKWATDEE
                     VLDYYAIGQCTPGIIAVNTATFIGYKRKGMIGAIAATMGMVFPSLVIITLIAAYFRHF
                     QDYAIVKHAFVGIRAAVAALILSAVVKMWDKSIKDSAGLIIFILSFAVIAFTNVSPIL
                     VIVVSAIAGILLSSNSKSKSDSNSNSANNSSSKCKRAGSK"
     gene            11238..11801
                     /locus_tag="PK733_00645"
     CDS             11238..11801
                     /locus_tag="PK733_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005212625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ09086.1"
                     /translation="MIYLTIFLEFFKVGLFAIGGGLATLPFLQELICKYGWITSAELI
                     DMIAISESTPGPIGINAATFVGYKTAGVMGGLTATIGLVTPSIIIVVVIAHFFMKFSE
                     QPIVRSGFYGLRPAVTGLIASAGFEVAKVSLININRYIKTYKLSELFNYKEIILFLVV
                     FYLISKYKKHPVFYLIGAAIIGILFKY"
     gene            11874..12527
                     /locus_tag="PK733_00650"
     CDS             11874..12527
                     /locus_tag="PK733_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ09087.1"
                     /translation="MDTKNNNKQHRLLEIFLTFFKIGAFTFGGGYAMIPLIEREVVHN
                     KGWVSTTDIIDYFAVCESIPGAIAINSATLIGYKTEGRKGAFAAAAGVILPSFLVITI
                     IAAFFTRFQNNAVVKAAFSGIRAAVTGLILIAAFKIGKASIKDYISLIITVVTVILTA
                     FLGVHAILSIIGGALTGFLIYNINPERISNISARTDSVDSSNSADSIDSSNSDKGGS"
     gene            12528..13127
                     /locus_tag="PK733_00655"
     CDS             12528..13127
                     /locus_tag="PK733_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ09088.1"
                     /translation="MTYLILLLTFFKIGLFSFGGGYAMIPLIEKEIESHGWLTASEFV
                     DIIAIAEMTPGPIAVNSATFVGYKTAGVFGGFFATLGVSLPSFIIILVVSQFFFRYRN
                     HPLNNAVFYGVRPVISGLIFSASIFVSKTALFKEIAVTNFFHNLALNILKVINPGGIL
                     ILAITLFCLIKLKMHPVLTIVISGIAGIVLYYVLPVFVF"
     gene            complement(13293..14492)
                     /locus_tag="PK733_00660"
     CDS             complement(13293..14492)
                     /locus_tag="PK733_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ09089.1"
                     /translation="MLGKLNLELIYDAASIQRWNDHIRPNKGFTELDKQSHKMMFAYV
                     LAKIEESDRKAVLNWRQLIEGGIFEFFHRIVLTDIKPPIFHMLMEKKGPKLNNWVLEQ
                     LEKQGFDQIKGSFFEKFRKYLQDPEYSFYEKRILKASHYLATNWEFEIIYNLNKNLYG
                     LEITKEQIANEIEEHYDLAGVQKLTLGKKTRNFLSFVGQLRFQQRWAQSPRVPETSVL
                     GHMLVVAILSYFCTLELDGCDKRVVNNYYAGLLHDLPEVLTRDIVSPVKRSVEGLEEL
                     IKEIESSQLEERVFPLIPAAWRNEIKYLIENEFSSRIMKDGKMEIITSDEINKNYNDD
                     MYSPVDGEIIRGCDHLAAFIEASLSISHGITSKYLKEGNESLYKRYRNKDIAGIDFGM
                     LFEYFKV"
     gene            14651..15556
                     /locus_tag="PK733_00665"
     CDS             14651..15556
                     /locus_tag="PK733_00665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ09090.1"
                     /translation="MELKYIAKEKDENRTVKSILKSELGLSERLIKKLKNTNGIFLNS
                     LPVYVVSRVQPGDLVHVSIELEEKCEDIIPENIHIDIIFEDEHMIILNKQPGIVVHPT
                     VSHPSGTIANAVMYHLSEMGKNLKVRPVSRLDRDTSGVIIFAKNPYVQTSLVRQMASN
                     TFIKEYLGIVWGVPDKMEGTIDMPIERKPDTIMLRHVSETGKPSVTHYEVLKTFDNAA
                     LLKFVLETGRTHQIRVHCQATGHPIIGDTLYFQEHISEIIARQALHSYKVSFIHPVTA
                     KPMEVFASLPDDMRRALEILENKHV"
     gene            15688..15933
                     /locus_tag="PK733_00670"
     CDS             15688..15933
                     /locus_tag="PK733_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ09091.1"
                     /translation="MSITKDNLANSKKTVDPNLENLAKESFTITELSALLNVTDHTLR
                     YYEKEFNLRIPRDSRGRRYYTPELSDIFLEIRKMKLK"
     gene            15945..16301
                     /locus_tag="PK733_00675"
     CDS             15945..16301
                     /locus_tag="PK733_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09092.1"
                     /translation="MTNNYCLQPVEANPSDSMQSNLDLDNIKKLLSDLEKQLHMNILS
                     GINSSIDSSSIHIINEISKSNEHLSKFIETNNTILDAKLERHFQSIDNSLSEWRNKNK
                     CGAIKQLFQRIGILPY"
     gene            complement(16499..17302)
                     /gene="spo0A"
                     /locus_tag="PK733_00680"
     CDS             complement(16499..17302)
                     /gene="spo0A"
                     /locus_tag="PK733_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOJ09093.1"
                     /translation="MLEKKIRVLIVDDNREFCDILSEYLDSQEDIEVVGIALDGMEGY
                     EQIIEKKPDVVVLDIIMPHLDGLGVLEKVNASLARNRPTFIILSAVGQDRITQKAISL
                     GAEYYVVKPFDMNVLVSRIKQLNGTASLIPQKEGYKANVNNFNIVSEKKDIEVEVTNI
                     MHDIGVPAHIKGYQYLRDAIIMVVEDMDIINSITKQLYPAIAKEYETTPSRVERAIRH
                     AIEVAWGRGRIDSINSIFGYTISVGKGKPTNSEFIAMVADKLRLQMKAG"
     gene            complement(17704..19029)
                     /gene="spoIVB"
                     /locus_tag="PK733_00685"
     CDS             complement(17704..19029)
                     /gene="spoIVB"
                     /locus_tag="PK733_00685"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOJ09094.1"
                     /translation="MRLYKKLLAKIGLYFIISAIILFLTCIYTAWSLPRDLVLVEGEE
                     YIYNVQSVFPVKLNFDKEETVQLGRNQVKPKRKLQFSNSVLLKTLNKGSANLEIKLFG
                     VIPLKLVKVNVVPNNKVVACGNTVGVKLKLDGILVIGMGEVEAAGGYRVSPIKDTGIK
                     PGDFILQVNRKIVNDISSLIEEINASEGKEMHLRYRSGDSFHDVQINPVKSIDDNNYH
                     IGLWVRDNTAGIGTLTFYDPETTYFGALGHGITDMDTGILIPVKWGEIIESNILAIKK
                     GENGVPGELKGIFAEDGDKLGSVTVNSSYGIYGQLYGSAISEVPGKTYPIAVRSQIRE
                     GPATIIANIMGREVEEYSIEIQKVSMKSVKGSKGMVIKITDERLLEATGGIVQGMSGS
                     PIVQDGRIVGAVTHVLVNDPSRGYGIFIESMIKEMYENSIVQNIKANAS"
     gene            complement(19180..20907)
                     /gene="recN"
                     /locus_tag="PK733_00690"
     CDS             complement(19180..20907)
                     /gene="recN"
                     /locus_tag="PK733_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOJ09095.1"
                     /translation="MLLRLEIRNIALVDEVGIELGDGLNILTGETGAGKSIIIDSINM
                     LLGERFSRDLIRTGQDKATLEAAFSVDNQRLADIYEKFGIEPESDGTLIISREYTTGG
                     RSTCRVNGRIVTVSMLKEIGSRLIDVHGQYDNQSLLRNENHIDLLDAFAGEEIEEAKN
                     EYSQLYNRRKEIKNNLQKLSGDEKDRERKIDLLKYQIDEIKNSKLKIGEDEELIKQKT
                     ILSNAGKIAETMEWLYNMLFSGSRADNAVYDEIVESVSKLESISEYDEVYSSIRSKIE
                     EIAFQMEDIQDEIRNAKNNIEFNPLQLEQIEERLDLIYRLKKKYGNNISEILQYCKKS
                     ERELDTIIKSEENINKLKKQLTGTEDALYGVAVIINQGRVKAAAILENKICGELEDLE
                     MKNVRFKVNIEMPEKQGEGTGIKFLDNGLDKVEFLISTNAGEPLKPLSKIASGGEMAR
                     IMLAIKAILADVDRIPTLIFDEIDIGISGKASQRVGEKLSYISKLHQVICVTHQAQIA
                     CMADNHYLIEKITEKGSTRTKVEKLTDKKTVDEVARILGGDSITVMTKNLAIEMLENA
                     KKLRKIKKN"
     gene            complement(21036..21485)
                     /locus_tag="PK733_00695"
     CDS             complement(21036..21485)
                     /locus_tag="PK733_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOJ09096.1"
                     /translation="MKYNRHAKILEIIEQNAIETQEDLVNKLKQEGMDVTQATISRDI
                     KELRLAKVMSDDGKSRYASVTQSESVISNKLLRVFNEAYVSSDYANNIVAVRTLPGMA
                     QAAASVIDSLRWPDIIGTIAGDDTIMIICRAEKIAEKLVEKFIKMVK"
     gene            complement(21475..22368)
                     /locus_tag="PK733_00700"
     CDS             complement(21475..22368)
                     /locus_tag="PK733_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOJ09097.1"
                     /translation="MKNIGVIVNRDRDVELIYTRILAENLCNAGIYALFLSEVVDMMG
                     LENKYIIKKDNEKEILKEAEMVVSLGGDGTFLKAARKVYRKSLPILGINLGNLGFLTE
                     VERNEIKDAVDCLTSGKYTIEERMMLEASIIRNDKVIAKDTALNDIVISRGALSRILH
                     VKTYINDVFVDMFPGDGLIVSSPTGSTAYSLSAGGPIVEPDIDLVIVSPICPHILYSR
                     SIITAGDRKVRALIDEDYHHKAMVTVDGQKGYEVRGGDIIDVQKSTYTIKLIRINPKN
                     FYNILRSKIYFRGERLKKNEV"
     gene            complement(22457..23272)
                     /locus_tag="PK733_00705"
     CDS             complement(22457..23272)
                     /locus_tag="PK733_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOJ09098.1"
                     /translation="MAKERLDVLLVEKGFFQTREKARNSIMAGVIFVNGIREDKPGSK
                     FDADVEILVKENLNPYVSRGGLKLEKALDCFGIDLQGRTAIDVGASTGGFTHCMLKRG
                     AIKVVAIDVGYGQLAWELRKDERVICMERTNIRYVKPEDVGVMADFASIDVSFISLTK
                     VIPPVINLIRSEGELVCLIKPQFEAGREKVGKGGVVREKDTHIEVLENITEFVLKTGL
                     CIKGLTYSPIKGPEGNIEYLLHVSKKVQPGFEDDITDIKDLIAKVVDESHKRL"
     gene            complement(23316..25262)
                     /gene="dxs"
                     /locus_tag="PK733_00710"
     CDS             complement(23316..25262)
                     /gene="dxs"
                     /locus_tag="PK733_00710"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOJ09099.1"
                     /translation="MALLLDRINSPDDVKGLNYNELEQLAVELREFLIEHISQTGGHI
                     ASNLGIVELTLALHKVFKTPIDKIIWDVGHQSYVHKIITGRKDKFESLRKLDGLAGFP
                     KTSESVHDCFNTGHSSTSISAALGIAEARDIKKENYFVISVIGDGALTGGIAFEALND
                     AGRSTTNLIVVLNDNEMSIARNVGGLSSYLSKIRTEPVYFKAKEDLDIMLNKTAIGKS
                     AAKVLHKAKDSIKYMITPGIIFEELGFKYLGPIDGHSIPKLVDVLNRAKMLKGPVLIH
                     VCTQKGRGYSFAEKNPDEYHGISPFEIETGEVKVHNGPNYSEIFGQELVKLAEDNSSI
                     VAITAAMPHGTGLNTFCEKFPRRFFDVGIAEQHAVTFAAGMASNGLRPVVAVYSSFLQ
                     RAYDQILHDVALQKLHVVLAIDRAGITGEDGETHQGLYDICYLRHIPDITILAPADYS
                     ELAAMLKYAVLEHNGPIAIRYPRGTGIKNIWDVVPDSWKPLKNDKYQLSGFGSIETGK
                     GVLVKEGKDITIIAVGNMFDSSVKAADILEKKGFSVELINPRFIKPLDENLILNSAIK
                     TRKIVTIEDGAIAGGFGSSIMELIDRAAVEYSNDKISGRPLIKVKAVGFPDEPIPQGS
                     RNSLYKKYGLDQESLVEQIEAFIV"
     gene            complement(25264..25971)
                     /locus_tag="PK733_00715"
     CDS             complement(25264..25971)
                     /locus_tag="PK733_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mg2+ and Co2+ transporter CorB"
                     /protein_id="HOJ09100.1"
                     /translation="MDDSYKRLPGDKKAKFRKNLLSTKKGNKRWVILITVSSFILSVL
                     FLSISNLILQDVSNTIAFILVLSIILTGIIFDIIGVAVTAADEAPFHAMASRKYYGAK
                     RSIKLIRNANKVSSFCNDVIGDICGIISGTASALIVVSITAGKSPAEKFIYGLLVSGI
                     VAAFTVGGKAAGKTIAISNSNYIVYKVGVILQFICGNINSKTNFSKDNKDNKDNKVNK
                     ANKVNKENKENKRKRGN"
     gene            complement(26873..27820)
                     /locus_tag="PK733_00720"
     CDS             complement(26873..27820)
                     /locus_tag="PK733_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ09101.1"
                     /translation="MSFNKTQFTIKYNSLLDQINSSLSEIIVKKETPEKSIYKAMNYS
                     LMAGGKRLRPVLSLAVCEMLGGNLNEVMPYACAIEMIHTYSLIHDDLPAMDNDDYRRG
                     RLTNHKVFGEARAILAGDALLNYAFENMLTNASKSNLDKNSTLCCKTSSCYRLEAVVM
                     AMSYIAEAAGVSGMIGGQVIDIESEGIEVSSELLEYMHKCKTGALIRAAVMVPAILVE
                     LDDEGKGDLEKYSEKIGLAFQIKDDILDREGSFESMGKKTGKDSGIGKSTFVTLYGLD
                     NSKAIMHDLISDAEEALKRFGPKADFLKSLAYYIIEREN"
     gene            complement(27810..28103)
                     /gene="xseB"
                     /locus_tag="PK733_00725"
     CDS             complement(27810..28103)
                     /gene="xseB"
                     /locus_tag="PK733_00725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOJ09102.1"
                     /translation="MKEENKTKEENKTFEESMNELEEIVQKLEKGELPLDDSIEYFQK
                     GIELSRYCSKKLDEIEKRITILIDNENGDVREENFNIDGGVQLSKDPEDADVF"
     gene            complement(28087..29325)
                     /gene="xseA"
                     /locus_tag="PK733_00730"
     CDS             complement(28087..29325)
                     /gene="xseA"
                     /locus_tag="PK733_00730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOJ09103.1"
                     /translation="MQYILSVTEVNRYIKEVIAGDMILSNIWIKGEISNYKNHYSGHM
                     YFTLKDEKSLIKCVMFKGQTYGLKFNPDNGMKVIARGYISVFERDGQYQLYVEEMQPD
                     GIGNLYLAFEQLKKKLEFEGLFDNRFKKELPYLPQSIGVVTSSTGSVIKDIINVASRR
                     FPNVNIKLYPVAVQGIQAASAISRAIRRLNELNCVDVIIVARGGGSLEEIWPFNEEIV
                     ARSIFNSRIPVVSAVGHETDFTISDFVSDLRAPTPSAAAEIIVPEKRLLKQKIADLRM
                     RMQNALLKNINFNKVHLLRITGSMVFKQPYNRLNQEKIRLDNLERKLVSTVLNQKDKS
                     KMRMLFLIDKLNSLSPLNVLARGYSMVKIKNNNVLLKSVKDVDSGDRLEISVSDGRVE
                     AIVDNISRGDNKGGNNERRE"
     gene            complement(29443..29862)
                     /gene="nusB"
                     /locus_tag="PK733_00735"
     CDS             complement(29443..29862)
                     /gene="nusB"
                     /locus_tag="PK733_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ09104.1"
                     /translation="MGRKLSREAAMKLVYQVEIQKDDREEQIRLSLEQPSFTKKDKIY
                     IEDVVKGVLDNTEKIDELIEKYAIGWKINRISKVDLSILRVSIYEILFREDIPYNVTI
                     NEAVEMAKKYSTSEAGAFINGILSKIPKTEIISTPET"
     gene            complement(29902..30132)
                     /locus_tag="PK733_00740"
     CDS             complement(29902..30132)
                     /locus_tag="PK733_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HOJ09105.1"
                     /translation="MDWRSLIEYYYAHQGAINGAAIGLVAAITILIFGFIKVFFIALC
                     VGIGYYIGKSISDDRHFLKNLKDKIFPPGMYK"
     gene            complement(30221..30853)
                     /gene="amaP"
                     /locus_tag="PK733_00745"
     CDS             complement(30221..30853)
                     /gene="amaP"
                     /locus_tag="PK733_00745"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOJ09106.1"
                     /translation="MNIFTRILLVLCAVIFIFVSSLSMIIALRHDVLFDVYRYLSTLI
                     ETNSYSTLVIILFSLAFLFIGIVTLISGVKLNRDKKSVSKYTNMGEVKISLATIDDIV
                     LNTAGKFGEIRDISTLIRKVADNVSITVRASVLPDVCIPALSGKIQEEVKFAVEESTG
                     ILVEDVRMFVQNIYSGHLPAAAGVKLEARGGKPGVSGEKPEVRDEKSNKS"
     gene            complement(31042..31464)
                     /locus_tag="PK733_00750"
     CDS             complement(31042..31464)
                     /locus_tag="PK733_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ09107.1"
                     /translation="MEEINTNIQGEIGSVKITDEVVSVIAGLAAIEVQGVASMSGGFA
                     GGIAEALGMKNLSKGVKVEVTEKEAIINLFIIVEYGARIPEVAWNIQENVKKTVESMT
                     GLVVTEVNIHVQGINIIKENKKEMSIKPDGEQIEQDDL"
     gene            complement(31665..32309)
                     /locus_tag="PK733_00755"
     CDS             complement(31665..32309)
                     /locus_tag="PK733_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOJ09108.1"
                     /translation="MVTILKKRQIVALSLVLMIVVAGYVQYSYKKSGFSKADDDTGKL
                     GEAVYVDNPDLLQDTGDKAASTKVGNEDELAQKETSEKHIVEKTENTVAASKEAENYF
                     AQTKLDKEISRSKETDKLGSIAADEGISQEIKDQAFTKMMAIIDNSDKEMRIEALIKK
                     QGFSDVIAFFADDGSVDIIVKAPNLSSTQVVQIADIVTRHANIEIDMISVKSKY"
     gene            complement(32437..33093)
                     /locus_tag="PK733_00760"
     CDS             complement(32437..33093)
                     /locus_tag="PK733_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HOJ09109.1"
                     /translation="MELVRKICDGVKNIIAGQDKKKIIENSIIIIIIGIIFLIAGSTL
                     FKSKNTNIIGNTGQGNFQKSSMESVNNSEEGILTSKSGGVYSIEKEVEEILSQIEGAG
                     KVSVAITYESGSEKVPAYDKRESHNSTQEEDDRGGKRNISQNESDSRVAYEEQPGGVK
                     RPIILKEMEPKVRGVVVVADGAGEPVVKENLVRAVQTLVDIAPHKVQVFARAKSAKNN
                     "
     gene            complement(33154..33813)
                     /locus_tag="PK733_00765"
     CDS             complement(33154..33813)
                     /locus_tag="PK733_00765"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HOJ09110.1"
                     /translation="MIEFLKEWVLSVTVVSILLILVEILLPSGKTKKFVNLVSGFILI
                     LTIVQPFFSKVFSNIDLDELFNRNNSYISAIAQVSADKDSKKLKEKQSAQILSIYMQR
                     VNERIVELAEEIDGVKEAASEVIVNEDYNSQYYGEIKKIYVYVYLKLKNKDKPQNRIA
                     PDVNIEKIRDIKIREGDETVIEDEKSVINDLNVELVKKLQGKIAGEFSIDIENIIISV
                     Q"
     gene            complement(33928..35184)
                     /gene="spoIIIAE"
                     /locus_tag="PK733_00770"
     CDS             complement(33928..35184)
                     /gene="spoIIIAE"
                     /locus_tag="PK733_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HOJ09111.1"
                     /translation="MKIKNKRNKTSILIKITEKAFLIIAILMVFQISCSKKTFAQESK
                     YDSTEAIEKARDELIRNQLQSSEIKALKEYLSEYATGAGGKLLDELNPDEIIKDAVKG
                     DLKLNISKLTKKILSYFIQEIYLNLNILLKLITIIIICAVLNNLQNSFIKEGVGELAF
                     FVCYIVIVSMLVIGFKEAASFGINVIEQMAGFMYATIPVMVTLLISSGSITSGGVFQP
                     ILIMAVETAATIIKNVFIPIVFLSTALSIINNVSEKLHLERLAGLIKKIGMAFLGIIL
                     TIFIGVVTIQGPVSAVADGVVNKTIKFAIGTFIPVVGGYLSDAADMVIGCSLLIKNAA
                     GVLVMLGVISICLIPVLKMSVIISLYKITCALTEPISEKRITNCINDVANSITLILGA
                     VAVVAFMFVISITVIIAAGNMTAMVR"
     gene            complement(35207..35593)
                     /gene="spoIIIAD"
                     /locus_tag="PK733_00775"
     CDS             complement(35207..35593)
                     /gene="spoIIIAD"
                     /locus_tag="PK733_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HOJ09112.1"
                     /translation="MEIIQVVIFSLLSTIIIVLLRAHRPEIAVQASLMAGIIIFALIV
                     SKIAPIFDFIRIYSGKANLNIQYLPVLIKIIGIAYITEFGAEVCRDAGENGIASKVEL
                     AGKIIIAMLAVPIVTTLLELIIRIMP"
     gene            complement(35616..35810)
                     /gene="spoIIIAC"
                     /locus_tag="PK733_00780"
     CDS             complement(35616..35810)
                     /gene="spoIIIAC"
                     /locus_tag="PK733_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOJ09113.1"
                     /translation="MDIDVIFKIAAVGILVSILNAVLKQSGRDELAMLTTLSGLILVL
                     LMVAKEIVNLFNLIKTMFQF"
     gene            complement(35861..36382)
                     /gene="spoIIIAB"
                     /locus_tag="PK733_00785"
     CDS             complement(35861..36382)
                     /gene="spoIIIAB"
                     /locus_tag="PK733_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein SpoIIIAB"
                     /protein_id="HOJ09114.1"
                     /translation="MLLKIIGSIMIMSGSSFIGFAMSSCCSKRPGQLKNLQNLMQMLE
                     NEIRYLSSIISDAFEKIYKSIDNPVNLFFKSTLEYLENREELNLAQAWERAIKDNIYK
                     TSLNKEDEEILVSFGKMLGNSDIEGQIKNIRFTIYQLSLQEQKAEELKKRNETMYRTL
                     GILGGATAVILLI"
     gene            complement(36447..37445)
                     /gene="spoIIIAA"
                     /locus_tag="PK733_00790"
     CDS             complement(36447..37445)
                     /gene="spoIIIAA"
                     /locus_tag="PK733_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOJ09115.1"
                     /translation="MSAKDKNINIIQYLSRGVREILEKVKPDKLFDLEEIRLRANKPL
                     IIQNYSEEWFVDSLGNLTKNIRDAFIVKREEILSTLEIMSKNSIYAFQEDIKNGFISL
                     SGGHRVGISGKVITDSGKVVNIKEFSSLNIRISKEVRGCSFKILKYIFNENKQVLNTL
                     IVSPPQCGKTTILRDLSRVLSDGAIDKGIKGMKVGLIDERSEIAACYKGIPQFDLGIR
                     TDVLDACPKSEGMLLMVRSMSPQVIITDEIGNKGDKESIIAILNAGIKLIATAHGYNI
                     SELKSRQEVLSMINEKVFERYIVLSNTQGPGTMEEVIDGSNMKVLYKREYAGITRV"
     gene            complement(37689..38234)
                     /locus_tag="PK733_00795"
     CDS             complement(37689..38234)
                     /locus_tag="PK733_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09116.1"
                     /translation="MNYLRERVAYVKGLAEGMQISETSNEGKLLRAIIDVLDDIALAV
                     DDIEETQDQQGEQIEDLDQDLAEIESMIFDYEDDENDDDDESYDYNYNTCSCGCDHEH
                     EQDHSFESDEGNEHDLCMVECPYCYNVVVLKKEMFDDEGKTIECPNCHKKIDIEWVCE
                     CEDCEEECEDCSDYEENDEDK"
     gene            complement(38322..38879)
                     /gene="efp"
                     /locus_tag="PK733_00800"
     CDS             complement(38322..38879)
                     /gene="efp"
                     /locus_tag="PK733_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ09117.1"
                     /translation="MISAGEFRNGVTFEMDGQVMQVIEFQHVKPGKGAAFVRTKLRNV
                     ITGGTVEKTFNPTDKMPKAHIERKDMQYLYNDGELYYFMDVETYEQVPLTKDALGDAL
                     KFLKESMTVKFLSHNSNVFGVEPPTFVELEIIHTEPGARGDTVTGATKPATVETGAVV
                     KVPLFVNQGDVIRIDTRTGEYMERA"
     gene            complement(39229..40347)
                     /locus_tag="PK733_00805"
     CDS             complement(39229..40347)
                     /locus_tag="PK733_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOJ09118.1"
                     /translation="MKEQNNVNRQNSDGDRYTEKRLARIREKLDENKIDAILITKREN
                     YIYLSGFTGTSAHLVITKKDAVLVTDFRYVEQASSQAPLFQIVRYKGDIALEINNLLK
                     SFGVSKLGFEAADVKYNKYVEFKEKFQTIELVPLNDIVESIRIIKDNRELGYIKKAVE
                     IADEAFNHILSYIKPGVGETEIAAEMEYFMKRQGGEGPSFQTIVASGVRSAMPHGVAS
                     EKELEYGDVIVMDYGAIYNGYCSDITRTVFLGDADSKIKKVYEIVLQAQIEALNGAFK
                     GKTGREIDEIAREVITRSGYGDNFGHGLGHGVGLEIHEEPRFAPAGDKIMENGMVVTV
                     EPGIYIEGFGGVRIEDMIVINDDEPLILTKASKKLTIV"
     gene            complement(40406..40837)
                     /gene="aroQ"
                     /locus_tag="PK733_00810"
     CDS             complement(40406..40837)
                     /gene="aroQ"
                     /locus_tag="PK733_00810"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOJ09119.1"
                     /translation="MKKILFINGPNLNMLGIREKSVYGNSTLEDIALYVSEKAKNLGV
                     EIEFTQSNHEGDIIDMIHQARGVYDIIVINPGAYTHYSIAIRDAIKSVDIPAIEIHLS
                     NIHAREEFRSRSVIAPVCAGQICGFGKGSYILALYAAVELL"
     gene            complement(40943..41797)
                     /locus_tag="PK733_00815"
     CDS             complement(40943..41797)
                     /locus_tag="PK733_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ09120.1"
                     /translation="MIRFGPSGNSQSFYDEGFKSSVQMPGWLKDMGLNAYEYSCSKGI
                     NIGRKTAEDLGDRARENNIFLSIHAPYYINLASDEPEKREKSIQYIIKTLEAASWMGA
                     GRIVVHTGSCSKVDRRWALDTSIEAMREAIARSDDMGYGNINVCPEVLGKMNQLGSLD
                     EILEICAIDERLIPTLDFGHLHARHGGNLNSTEDFDRVLNRVEDALGNERLKHIHIHF
                     SRIEFTAGGEKRHRTLSETEFGPEFEYLAIAIKKKNMEPVIICESRGNMAEDALKLKE
                     IYSKICQM"
     gene            complement(41837..42574)
                     /locus_tag="PK733_00820"
     CDS             complement(41837..42574)
                     /locus_tag="PK733_00820"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOJ09121.1"
                     /translation="MINFIKYIIVFFVGIFFGWFNHVIIKNLSEEKPFGKNWFHEKSS
                     TIEFKCLTYSPKSLLLEIVTGVVFVILYSKYSITADFFFFAYLMIILIIMFFIDLKTK
                     TIPNELVVAGLIGGLAVFTYNIFLPLSIYGSKKWWEPLLGLLPGSGVLFLIAILGMII
                     YKSDEVMGMGDVKIFAPIGIFLGWKMCLLTLVVSIFLGGFSSILLILFRIKKRKDVIP
                     FGPFIAIAVFITILFGNNIWEWYFSWL"
     gene            complement(42643..43521)
                     /locus_tag="PK733_00825"
     CDS             complement(42643..43521)
                     /locus_tag="PK733_00825"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOJ09122.1"
                     /translation="MNIVHSVTGKTKLLGVIGNPLSHSISPQLHNTLSCCLRIDAVYV
                     PFRVGEGYLEYAVKGLKALDVLGFNVTIPYKNDVINYIDSVSREASLIGAVNTVKNID
                     GRLYGYNTDAAGFVNSFKEEAGTGFKDKNVVIIGAGGAARAIATGIAFEGAGRISIIN
                     RTISRAAEIADIINKNILPVAGYYDLQDNNISEAIKQADIIINTTSLGMYPDINKLPL
                     TIPLNFSTNQIIYDVIYNPKKTRLLQEAEKQGCKAVNGLGMLIYQGIKAYEIWMEMDI
                     PGEIVKSLFEAFREYL"
     gene            complement(43672..44196)
                     /locus_tag="PK733_00830"
     CDS             complement(43672..44196)
                     /locus_tag="PK733_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOJ09123.1"
                     /translation="MTEKFFPCLIVDKFQDINLDMLQKKRIKGLILDIDNTLVPSFVK
                     EADNNVLEWIERLKNRGFKVCIVSNATKKRVDRFNEKLCLEAIYRASKPGRRSFKKAL
                     KVLGLKPAETAVVGDQIFTDVYGGNRLGMYTILVKPINRKETLFVMLKRYPERYILNR
                     YYKTVGQAGSGSKK"
     gene            complement(44319..44546)
                     /locus_tag="PK733_00835"
     CDS             complement(44319..44546)
                     /locus_tag="PK733_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09124.1"
                     /translation="MELYFHVYELTREKLCCFKFYYYKNDCTSSIYGDVRVEYDEGRR
                     KTASFDPKSDLLLSGKEKDSIVAGVLKNVYI"
     gene            44875..45945
                     /locus_tag="PK733_00840"
     CDS             44875..45945
                     /locus_tag="PK733_00840"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOJ09125.1"
                     /translation="MVNQIRIEKTKSPKNKPNQSKLGFGKYFTDHMFVMEYSTEKGWY
                     DPKIVPYGPLALDPSTMVFHYGQAVFEGLKAYRAKDGRILLFRPFKNMERINISNERL
                     CIPPIDVDFIVDTIKTLVKLEEDWIPTEEGTSLYIRPFIISTDQYLGVKPSSTYKFIV
                     ILSPVGAYYPEGINPVKIYIEDMYVRAVKGGIGYAKTPGNYAASLKAQVDASKKGYTQ
                     VLWLDGVDKKYIEEVGTMNVMFKINGEVVTPSLEGSILAGVTRDSAIQLLKSWNIKVA
                     ERKISIQEIYDAHAAGTLEEAFGTGTAAVISPIGELNWDGKIITINNGVTGETAQTLY
                     DGITGIQSGKIEDAFGWTVEVK"
     gene            46105..>47271
                     /gene="ylbJ"
                     /locus_tag="PK733_00845"
     CDS             46105..>47271
                     /gene="ylbJ"
                     /locus_tag="PK733_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HOJ09126.1"
                     /translation="MAVNMGNRMVSIRLKQKAAKSKCKIESIKAFILPIISIAFILLL
                     IIFSKTAVSSALKGIDLWLNVVFPSLFPFLTASELLNGTSFVKAAGILLEPVMRPLFN
                     VPGCASFPFATGMTSGYPVGAKVVASMKENKLLNKWDAEKLLAFCNNSSPLFIIGAVS
                     TGMLKMPQIGPLLLICHLAAGITVGFIYRFIPVNPKNVTLSGGRKPVSGKSGSTSNID
                     FGSLLGNAVRNSVTTLLVIGGFIILFSVIIGILMESGILNSFAGLLYTLLKFTGLPKE
                     LYISLISGFIEITTGLGMLCSSPNITLNMKLITVSAIIGWGGISVHMQVMSVVRNAGL
                     SIKPYLMGKCVQSILSAIYTMGGLKLTGLYPETAALVFKNFYTPNLRTWNQYLLE"
BASE COUNT        13936 a         9873 c         6877 g        16585 t
ORIGIN      
        1 tgtttggatt atattcgggg ctgatactcc acttgccaat gtgaccggtg aggtacccat
       61 gttcttttaa acctcttacc catgtataat ctgttggctg cagggttgaa acttttaagc
      121 cattgttgta gttccatagt gccccaaatg tttcaggtcg tctgccattt aacaatgact
      181 gtcttgcagg gcagcataca ggtataggag tataagcatt tgtgaaccac ataccttcac
      241 atgctagttt atcaatattg ggcgtatgta tgggatattc attactatac ccaatgcaat
      301 cataacggtg ttgatcagct aaaataagta aaatattggg cggcttaaca ttttgcaaaa
      361 tcagataccc tccatctaga aaattcgctt cgctcatgac ttaagcattg atgcgaattt
      421 tcttgttcgc ttacttaaat aaaattatgt taagaagcta tgtaaccctt gcgctacata
      481 gcttctaaca atctacagca cctatagtta ttatagtata tacttataaa ttacatgagc
      541 taccccatct tcatcatttg acagcgttat ataatcagct ttttccttta cagcatcatt
      601 tgcattttcc atggcaacac caagaccggc atactcaatc atggaaaggt cgttcagtcc
      661 atcacctaca gctatcattt ctcttatgtt tatattgaag tgttccccca gcttttccat
      721 agcctttgcc tttgatgctc ttttatctac aaattccagg aaatatggtt gtgaggtatg
      781 aaaattaaca ttttcgctta aatagccgct tacttcatta aggtatctgt taatcgtctc
      841 aacctcgtca taccagagta ttttcgtaac tccttttgca attgtctttt caatactatc
      901 tctatcattt cccaataata acggtgtaac agttgatatg ctgctatatt tataagcttt
      961 gtcacttagc tcacttgcgt ataatttatt atcagtccat accatgacag ttgttcccag
     1021 ttttttgcca agttcaatta tgttcatggc atcctcatta tttaagctgc attcaaacag
     1081 gatatctttt gacttccaca ttattaccat tgcgccatta tacgttataa aaggcaaatc
     1141 atgggttagt tctaatttct ctattaatga ttttaatccc tgtatgggtc tgcctgtaga
     1201 aatagtaaaa atcatccctt tttctatccc taggtttata gctttgatag tctcatgagt
     1261 taagtttcct ttgctatcaa gcaatgtacc atcaatatca actgccagta acttataagc
     1321 catttaatta ttttctcctt taatatttca catttcacat tgcaataacg ccacacaata
     1381 gtatcattag gcaagtatca aacaagaact ctattagcaa atgataaaga actatctctt
     1441 tgttcctaat tgttctagcc cctctgttct tgtgttccta tttctctgcc attttctcaa
     1501 gaattatatt gcttctttca ataaatttgg tcattgcttc aggctcaagc tgcttatggc
     1561 tcatcatagc cagatcataa acctgtcggc aaagcaaatt cacatcatct tttttgtcct
     1621 ctttatcttt catctccagt attaactgta ttaatctatt cttgtcattc agcaccaggg
     1681 tttcttcctt tgggaacatg tggctcatat cctgaccacc gaagtagcgg ctgaagtcct
     1741 gcattcttct tgactgttcg gaaagaagaa tcattcctgg gacagaagca gttttcaagc
     1801 tttcaacttg tacctttagc ttttcatcgc ttacagcttc cttaaatgtt ttttccaaag
     1861 cttcccggtt ttctttttca gccgggctgt cgttttcttt tccatctccg gctttcaggc
     1921 tttcagaaat atctgaatct atccggctga aggttacgcc gctttcatgc atttccatga
     1981 actgaataaa atggctatca attaaagtat tcaagatgac ggcttcaagc tcattttcct
     2041 caaagatttt tatatattgg gcctgctgtt tttcatcggt tacatagaat accttgtttt
     2101 tatgctttga ttcattgcgt gacaggtaat ctttcagtgt aatataatca ccttttgtag
     2161 ttttataaac aactatatct ttaactctat catagaattt ttgttcacgg atgcagccat
     2221 attttacaaa tggattaatg tcatcccaat acttgttata attatctctt tcattttcag
     2281 aaagggatac caatttgtct gctacttttc ttgtaatgtg attggatatt ttacttacat
     2341 aaccatcatt ctgtagaaag cttctggata cattcagggg taaatccgga caatctatgg
     2401 ttccctttag aagcataagg aattcaggta taacctcttt tatgttgtca gctacaaaaa
     2461 cctgattgta gtaaagctta atttgtcctt ctatggtttc aaactcatgc ttcaatttgg
     2521 gaaaatagag gattcctttt aaattgaaag gataatccat attcaggtga atccaaaaca
     2581 gaggatcatt aaaatcatgg aatacctttt tataaaattc cttatactct tcatcagtac
     2641 agtctttagg ctttttcaac cacaaaggct gagtatcatt tattggtttt ggactgtcat
     2701 catcatcctc atctttcatg tcatctttaa tatcatcctt tatatcatcc tttatgtcgg
     2761 ccgtatcagc atcttttgca tctttaccag tttcttctgc atctaagctt gtttcttcta
     2821 catttgtatc agagtctttt aatttaccat cctgtttttc gctctcaatt attttatctt
     2881 tagtatcctt tttcttttta tcttcggcat tttccagata aatttcatat ggcataaatg
     2941 cacaatactt ggtgagaacc tctctcattt tatattcatc cagatattcc tgcccatctt
     3001 cagaaacaaa tagagttatt gttgtcccac gttccctgcg gtctgactca tccatctcga
     3061 actgatcatt cccggtgctt gtccatctaa cagcctgagc accttcctgg caggataaag
     3121 tgtctatttg caccttttca gataccataa aagccgaata gaaaccaaga ccaaaatgtc
     3181 ctattatttg attatcctgg tccattttat ctttatattt ttctataaaa tcagtcgcac
     3241 ctgaaaaggc aatctggttt atatattttt ttacctcttc atcggtcata ccaattccat
     3301 tatcaatgaa ttgaatggtt ttcttatctt tgtcaacaat aacagtaatg cggaattgct
     3361 catcatcatc agctttagct tctcctatgg aaaccagcct tttgaacttg ttgattgcgt
     3421 cagtgccatt tgataccatt tcacgtgcaa agatgtcttt atctgagtaa agccaccttt
     3481 ttattatagg taaaatattt tctgtatgta tggataaatt gccaacttca cgggccatac
     3541 aaatacctcc tcctaatcaa tatattgaca aatgcgttta tttatgctta tggcattcat
     3601 ttgcctttcg cacaataatc taattataat ctagctatat tctaaattat aatataaatt
     3661 gtttgtgttt actcaattag agtataaaaa tcctgcctat ttattttaca atttgttgaa
     3721 ggcttttgtc aaggattttt tagcactctc ttgtatagag tgctaacaat tattcaagct
     3781 ggctattatg ctgcttactt atatctcttc taatatgaag ggtgtaaaac acttttttta
     3841 tcaacattac taataacaag ataattacac cagtaagcac tatggttgca ccgggtttta
     3901 catctgcata gaaagaaatt atcaagccag aaatagttga tataactgca aaaacaattg
     3961 agtatataac tgtctctttg tagctttttg agataagcat agatgaagca acaggaacaa
     4021 tcataagtga agatacgaca agagttccga tagtacgggc tgaaatagat attactattg
     4081 ccgtcagaag cataaatata aagtttactc ttgctacagc tatacctgcc aatttcgctg
     4141 attcctcatc gaaagttatg caaaataagt ctctgtacat aaaaataaaa gcaaataata
     4201 caacaacact taatattatt actgttagca gttcgaaacg actgacggca acaatacttc
     4261 caaacaggaa gcttgtaaaa gccgaactat tcttaacaaa tcccgataaa atacctgcca
     4321 gtccaatacc tgctgccata ataattgcca gtgatatttc cgcatattga gaaaaggact
     4381 tgcggatttt ctcaattccg aaagaagcca gtatggaaaa tgttacagct cctgctacag
     4441 gatttacccc cgccacaagt ccggcagcta cccctgcaag tgaaatatgg gacagggtat
     4501 ctcccatcat ggacaaacgc tttaaaacaa ctactacacc aatacatgga attataattg
     4561 atattaacaa gcctactata aaggcatttc tcataaactc atattgaaat atttctgcca
     4621 tatttttatt tcgtctccct ccacagaaac tcaaatttct atataccacc agttttattt
     4681 tttagtttag cattgcggta gcatgtagtg cattcatgtc tattcaaatg aatgttaatt
     4741 ttatacccat acaattcatc tattaaatct tctgttattt catccttagg actaattatc
     4801 tttacatctt tattacctgc aaatcctatt ttgtttgcat ggactgttat tgcacctatg
     4861 tcatgggtta ccattacgat agttatgccc agttccttgt taagtttagc aagaagacag
     4921 tataccgcct cctcagattt tgcatcaatg cccgttgttg gttcatcaag aaagactatt
     4981 tcaggattat taacaagcat gcgtgcaata aaaacccttt gctgctgacc tcccgacaga
     5041 ttaccaataa gttttttggc attgtcttcc atttccacaa ttttaagagc attataaact
     5101 ttttccttac gctgcttctt aagatttgca gtaaataaac cggatatttt ggcttttctg
     5161 taaatattag aaccaacgat ttcctcaaca gtagcaggaa aactcatatt gaaagagatt
     5221 gctctctggg aaatgtatcc tactttgctc cagtcaggaa aattctttac attatatccg
     5281 aatatattta tatttccccc attaggcgaa agaaagttca aaaataattt tacaagggtg
     5341 cttttacccg agccattggg tccgattatc ccaaaatagt cacccctgca aatatcaaaa
     5401 cttacattat caaatactgt tttgccatta tagctaaaac ttagattcct tacatccaat
     5461 gccttatcca tctacaccat ccatattctt atttatgttg agacaagcaa gagggaaaac
     5521 gaataagaga gcaaagagag acggttcttg acttgtacat aggaggagca agcaagggca
     5581 gagctcattt atccttaatt gcccttagtt gtcctcactt atcctcactt gtttcctgct
     5641 tacttgacta gaaccgcccc tgcttgtctc cccttgtctg tcctttcagg caagttaaag
     5701 aactgtccac acttgtccct ttactttaac gcccttttca atgcttccag attctccctc
     5761 attgccgaaa aataatcctt accttccttt agctcttttt cgctaagact tgctatggga
     5821 tttaacactt caacttttgc ccctgcggac tgggcgattg catttgcaac ctttggattt
     5881 acaagtttat cgaaaaatat tacactaaca ttgttatctt tagtgaagtt tgctacatct
     5941 accattcttg cagcagtagg ctcagcctcc gcatccaaac cggaaatagc cacctgattt
     6001 aagccgtaag cagcagccag ataaccaaaa gcttcatgag aaacaacaat atctttcttt
     6061 ttaaatccgg atgctgcttc cctaaattca ctatcaagct tctccaactc tctggagtat
     6121 ttttcaaaat tcttttcata ataatcctta ttgtctggat caactttaac tagagcatct
     6181 gcgatggctc tcatctgtat ttttgccatg gctggatcca gccacacatg agggtcatat
     6241 atatgctttt tttcttgatc agcctgtttc tcgccatcct tttcatcatg cccatgctca
     6301 ctctcgtgct catggtttgc tttacgaagt tcaatatctt ttgctgcttc aacaataata
     6361 aggttcttgt tgcttatact ctccaatact ttgtcaaccc agccttccat gcctgctcca
     6421 ttataaacga atacatccgc tttctctata ctaataatgt cctttggtga cggttcccag
     6481 ccatggggat ctgacccggc aggcagcagg ttttttacag taactttatc gcctcctatt
     6541 ttacctgcaa aatcatatac tacataaaaa ctagcagtca cagatatttt attttccgtc
     6601 ccagcctgtc cttcaacact ttttctgcca gtacagcctg taaaaatggt tgcacatatt
     6661 aatgtcatta ggaatattgg caataaaatg attattgtta ctttgtaagt tttcacatta
     6721 cttttcatcc tgttttcacc tcttcatttt tacaactaca ttaatgcttc cttttcaata
     6781 tagccgtact agatagattt taaactaata aactgctttt ttcaacagga tagtttatat
     6841 ggggtgcatt tactgaattt gaagaaatat ttaccaggga ctgtcgttac agtgcttaga
     6901 gcctttaaat tcagctacgg gcaaaatcaa actcacaaaa taatttgaat aatagtattg
     6961 taattatttt gctcaaacac tgattttgct tttcctccgc tcagattaaa aggctctaca
     7021 gcaccttcac ttatgcccct taccaataat ttcttcaaat aaattaaatg tacccgttca
     7081 tattgagtgt actaataatt ttagcttttt gggtgtgaga gtctccgcag ctcttgccac
     7141 ctcttctttt gatggctctc ttacattagc tgccgggtac tcaagcccca gtttatccca
     7201 cttaaatgct cccatacaat gataagggag tacttctacc ttttctacat ttttcaggct
     7261 ctcaataaat tccccggcaa gtcttaaatc ctcttcgtta tccgtgtagc caggtaccag
     7321 tacatatctt atccataagg gtatcccctt tttcattgta tataaagcaa actttttaat
     7381 tttatcattt ccaactcctg ttatctccct gtgttttgct tcggaagcat gtttaatatc
     7441 aagaagtatc aggtctgtgt attcaagcag ttccatcacc ttttcaacct ctgcaaaccc
     7501 acttgtatca agtgcagtgt gaatgttcaa ttctctgcat cttttgaaaa gagcagttac
     7561 aaaatccacc tgtattaatg gttctcctcc cgtaactgta attccgcctc ctgaggattg
     7621 catgaagtca atatattttt cagtctgggc aacaacatca tctacagaat attcccgtcc
     7681 gccgctcaca tcccatgtat ccctgttatg gcaatatatg cacctaaggg gacatccctg
     7741 cataaatact ataaatctta ttcctggacc atcaagagtc ccaaaggttt caaaagaatg
     7801 tattctcccc tttatctcca tatgtttacc ttacaccttg cctttctcat actctctcat
     7861 gtattgtcct gttaataaca tccagctgct gtgcttttgt taatttaata aagtttaccg
     7921 cataccccga aactcttata gtaagctgag gatacttttc aggatgttcc atggcatcta
     7981 gcaatgtttc cctgttaaaa acattaacat tcatatgatg gcctcccttt ttaaagtagc
     8041 cgtcaagaag agccataaga ttattaagtc tggcttcttc atctctgcca agagctttag
     8101 gtgccattga aaaagtaaag gatattccat cctgagcatg tctgtatgga agtttggcaa
     8161 cagaactgat tacagccagt gccccatttg tatctcttcc atgcatgggg tttgcgcctg
     8221 gagcaaaagg ctctcccttt tttcttccat caggagtatt tcctgttttc tcaccataaa
     8281 ccacatttga tgttatggta agtattgaaa gtgcagggac ggagttccta taagttctct
     8341 gttttctgag cttatccata aatagtttta caaccattat agcaatagaa tcaactcgtt
     8401 catcattgtt accgaacttt ggataatttc cctctaccag ataacccact actgtgttat
     8461 tttcatctct cacaggtttt acttttgcat actttatagc acttaaagaa tccactacta
     8521 cagacaaacc tgcaatccca catgccattg ttctatatat gtccttatcg tgcagtgcca
     8581 tttgaagact ttcataggaa tacttgtcgt gcatataatg aattaaattc aatgtgttta
     8641 tatataggcg ggcaacccag tccaacacca tatcaaacct attcattaca tcttcataat
     8701 ccagatatcc ctctcctaca gcttcaaacc gtggagcaat ctgcgccccg gtcatttcat
     8761 cccttccacc atttatagca tataacaaag cttttgccaa gttgcatctt gcaccaaaaa
     8821 actgcatttg ttttcccaat ttcatagcag atacacagca ggctatgcca taatcatctc
     8881 cccaataagt cctcatcaaa tcatcatttt catactgtat tgaactggta tgaacagata
     8941 cctcagcaca gaacttttta aattcgcaag gaaggtgtac cgaccatagt actgtcatat
     9001 taggttctgg tgcaggtccc agattgtaca gagtatttaa aattctgaaa gaggattttg
     9061 ttacaagagt ccttccatca aggcccattc caccgatact ttcagttacc caggtaggat
     9121 caccactgaa aagagcttcg taatcaggtg tcctaaggaa tctcactatt cgcagtttta
     9181 taacaaactg atccattaat tcctgtactt catcctctgt gatagtgcct tctctcaggt
     9241 cacgctcaaa atatatgtca aggaaggtgc ttactctacc aagactcatg gcagcgccat
     9301 tttgctcctt tacagctccc aaatatgcaa aatagagcca ttgtaccgct tctctggagt
     9361 tccctgcagg cttggatata tcaaaaccat agcttgacgc catttcctta agaaatccga
     9421 gagcttttat ctgttcctgt atttcctctc tgtcccttgt agtatctgca tctatataat
     9481 caaactccag atgcacaagt tcatttctct tttcctctat aagcctgtcc acgccatata
     9541 ggggaaccct tcgataatca ccaattatcc tgcctcttcc ataggcatca ggaagccctg
     9601 ttattatacc tgcttttctg gcccgcctca tttcagctgt ataaacatca tatacccctt
     9661 gattgtgtgt ttttctgtat tcagtaaaca ctttaataat atcagagtct actttcttac
     9721 cataagcttc gctgcccttt gcaaccattc ttataccgcc aaaaggcata atggctcttt
     9781 tcaaaggttc gtcagtctga agccctgcaa tcacttccaa atccttatca atatatccgg
     9841 gtttatgaga aacaattgta gaaacagtgt gggtatcaat atcaagtaca ccgtcttttt
     9901 ccctttcctt ctttagaagc tctgaaacct tattccaaag ctttctcgtc ctttctgaag
     9961 cacctttcaa aaaagatcta tctccctcat aaggagtata attaagctgt ataaaatccc
    10021 ttaaatctat ttcagaacac cagttacctt ttttaaaatc tttccattgt tccatatttc
    10081 tgttcatctg ttccatattt ctgttcatcc tttctattga ttaataaggg catcacttcc
    10141 taaatctgca aaactaaaat aattccttat taaataatat tacccttaat aatcctttta
    10201 aaaccttaag gcaaagggac aggcacattt tcttcctaag ctgaatttaa aggccctggg
    10261 cagcataacg gcaacttttg gaaggcgtat aacatgatga cgaaatatta gagcaaacat
    10321 agagattgtt ggtatagaag cacagagaaa agctgtaatt ctgcacatgg acgtgcagag
    10381 caggcatctg agccatggat ggcgaatatg ccgggcaggc ttttctctgg gaaactatgc
    10441 ctacagtctc tgctctcagt tcttgatgtg cagcgactgt tttggcatca tgtcatatgt
    10501 ctttcatata atggcttaaa ttgacgccta tgataaatac accccatcta taattaacat
    10561 gtttacaatc ttaatttatc tctgctataa taaaagaaaa tcttgatttt taattatgta
    10621 taaataaagg tgtagtatat gaaagaactt ttggaattgt ttgtaatttt ctgccggata
    10681 ggcggtttta cctttggggg cggatatgct atgcttccaa taatacaaaa agaaatagta
    10741 gaaaagagaa aatgggctac agatgaggaa gtactggatt attatgcaat tgggcagtgc
    10801 actcctggca taattgcagt caatacggca acctttatag gatataaacg taaaggcatg
    10861 atcggcgcaa tagccgcaac aatgggtatg gtttttccat ctttagtaat aattacttta
    10921 atagctgcct attttagaca ttttcaagat tatgcgattg taaaacatgc atttgtcggc
    10981 attagagcag ctgtagcagc attaatactt agtgcggtag taaaaatgtg ggataaatct
    11041 attaaggatt ccgcaggcct aataatattc attctctcct ttgctgtaat tgcttttaca
    11101 aatgtttcac ctatattagt cattgtagtt tcagctattg ccggtatttt gttaagctct
    11161 aatagtaaaa gcaaaagtga cagtaacagt aacagtgcta ataacagtag tagtaaatgt
    11221 aaaagggctg gtagcaaatg atatatttaa ctatttttct agaattcttt aaagttggct
    11281 tgtttgcaat tggcggagga cttgcaactc ttccatttct tcaggaattg atctgcaaat
    11341 atggctggat tacttctgcc gaacttattg atatgattgc tatttcggaa tctacgccgg
    11401 gtcctattgg cattaatgca gcaacttttg ttggttataa aactgcagga gttatgggtg
    11461 gattaactgc aactattggg ctggttacac catcaattat tatagtcgtt gtaattgctc
    11521 atttttttat gaaattcagc gaacagccca tagtaaggtc gggtttttac gggttaagac
    11581 ctgctgttac aggtcttatt gcttcagcag ggtttgaggt cgcaaaagtt tcattaatta
    11641 atattaatag atatattaaa acatataaat tatcagagtt atttaattat aaagaaatta
    11701 ttctgttttt ggttgtattt tacctgataa gtaaatataa aaagcatcct gtattttatt
    11761 tgataggtgc tgcaattatc gggatattat ttaaatattg atcaattctt tttgcatgag
    11821 agttatgtct tgtaaattaa gtaatattgt gtattttgta aaggaggtat atattggata
    11881 ctaaaaacaa taacaaacaa caccgactgc tggagatttt tttaacgttt tttaaaattg
    11941 gagcattcac ctttggcggt ggttatgcca tgatacccct tattgagagg gaggtagttc
    12001 ataataaagg atgggtaagt actactgata taattgacta ttttgccgta tgcgaatcaa
    12061 tccccggggc aattgcaatc aactcagcta ctttaatagg ctataaaact gagggtcgga
    12121 aaggagcatt tgcagcggct gcaggagtaa ttcttccatc atttttggtc attactatta
    12181 ttgcagcttt ttttaccaga tttcagaata acgctgttgt aaaagcagca ttttcaggta
    12241 ttcgagctgc agttaccggg cttatattaa tagcagcctt taaaatagga aaagcatcaa
    12301 taaaagatta catatctctt attattacag ttgtgacagt aattctgaca gcctttttgg
    12361 gtgttcatgc aatactttca ataattggag gagccttaac aggttttctt atttacaata
    12421 taaacccgga aagaatttcg aatatatcag ccaggactga ttctgttgat tctagtaatt
    12481 cagcagattc tattgattct agtaattcgg acaaaggagg tagctaaatg acttatttaa
    12541 tactactttt aacattcttt aaaattggtt tatttagttt tggcggcggt tatgcgatga
    12601 ttccccttat agaaaaggaa atagagtccc atggctggct tactgcttca gagtttgttg
    12661 atattatcgc cattgctgaa atgacaccgg gacctatagc agttaattct gcaacattcg
    12721 taggttataa aactgcaggt gtttttggag gtttctttgc aacacttgga gtatctctgc
    12781 catcatttat aattattctt gtagtttcac agtttttctt tagatacaga aaccatccat
    12841 taaataacgc agtattttat ggagttcggc cagttatctc aggattgatt ttctctgctt
    12901 ccatctttgt ctcaaaaacc gcattgttta aagaaattgc tgtaacaaat ttttttcata
    12961 accttgcttt aaatatatta aaggtaatta atccgggagg aatactaatc ctggccatta
    13021 cgctgttttg tctgataaaa ttgaaaatgc accctgtatt aactattgta atatcaggca
    13081 tagcagggat tgtgctgtac tatgttttac cagttttcgt attctgatat tattccgtaa
    13141 aatatagagc ttccagcatg gtacaaaatg tggtacatag aaaaaagaaa gcacttcatt
    13201 aactctcctg caatatgctc cgaatcagta gcacttcttc cattaccaca tatcaatact
    13261 ttattgtcta aatcatagca ccctcacaat tcctatactt taaaatattc aaacaacatt
    13321 ccaaaatcaa tacctgcaat atctttattt ctatatcttt tatataatga ttcattgccc
    13381 tctttaagat attttgatgt aataccatga gatatggaaa gggaggcttc aataaatgca
    13441 gcaaggtggt cacaacctct tattatttcc ccgtctacgg gagaatacat gtcatcatta
    13501 taattcttgt taatttcatc tgaagtaata atttccattt ttccgtcttt cattatcctg
    13561 ctgctgaatt cattttcgat aagatacttt atctcatttc tccaggctgc aggtattaaa
    13621 ggaaatactc tttcttccag ctggctgctt tcaatctctt ttataagttc ctcaagtccc
    13681 tccactgaac gctttacagg agagacaata tcccttgtta gaacttccgg caggtcgtgt
    13741 aaaagtcccg cataataatt atttacaact cttttatcac atccatccag ttccaatgta
    13801 cagaaatagg aaagtatggc gactacaagc atatgtccta aaaccgatgt ttccggcact
    13861 ctgggtgatt gagcccatct ttgctggaac cttagctgcc ccacaaagct taaaaaattt
    13921 cgtgttttct ttccgagggt aagtttttgc actccagcta aatcataatg ttcttctatt
    13981 tcgtttgcta tttgttcctt tgtaatttcc agaccgtaaa ggttcttatt caagttatat
    14041 attatttcga actcccaatt tgttgctaga taatgggatg ctttcaatat cctcttttca
    14101 taaaaactgt attcaggatc ttgaagatat ttgcggaatt tctcaaaaaa gctgccttta
    14161 atttggtcaa aaccttgttt ttcaagttgt tccagcaccc aattgtttag cttaggacct
    14221 tttttttcca tgagcatatg aaaaattgga ggcttaatat ctgtaagaac aattctgtgg
    14281 aaaaactcaa agatgcctcc ctctatcaat tgtcgccagt ttaatacagc ctttctgtca
    14341 ctttcttcaa ttttcgccaa tacataagca aacatcattt tatgtgactg tttatccagc
    14401 tcggtaaaac ccttattggg ccttatatgg tcattccatc tctgtatgct tgcagcatca
    14461 tatattagct ccaagttaag ttttcccagc atatatttca cctcatttat tatttgggcc
    14521 gttttgctat atatttacaa tatatcagat taagttacat agttcaaggg gatagctgca
    14581 aatttcaact tgttgacgct gaatgagttt tttgtcataa taaatatatt aattactaat
    14641 ggtggtagac atggaactta aatatattgc aaaagaaaaa gatgaaaata gaacagtaaa
    14701 atcaatatta aaaagtgagc ttggactttc agaaaggctg ataaagaagc ttaaaaatac
    14761 caatggaata tttttaaact ctttacctgt atatgttgta agccgtgttc aaccgggtga
    14821 tttggttcat gtttctattg agctggaaga aaaatgtgaa gatataatcc ccgaaaatat
    14881 tcatattgac attatttttg aagatgaaca tatgataatt ttgaataaac aacccggtat
    14941 agtagtacac cctacggtaa gccatccttc aggtactatc gctaatgcag taatgtatca
    15001 tctttcagaa atgggtaaaa atttgaaagt cagacctgta agcaggcttg acagagatac
    15061 gtcgggagtt attatatttg caaaaaatcc ctatgtacag acttctcttg ttcgtcaaat
    15121 ggcgagcaac acatttatca aagaatattt gggaattgtt tggggagttc cagataagat
    15181 ggaaggcaca atagatatgc ccatagagcg caaacctgat actattatgt taaggcatgt
    15241 ttcagagact ggcaaaccat cagtaactca ttacgaagtc ttaaaaactt ttgataatgc
    15301 agctttactt aaatttgttc ttgaaacggg aagaacccac caaatcagag ttcactgtca
    15361 agccacaggc caccctatta taggtgatac actctatttt caggagcata tatcagaaat
    15421 tattgcaaga caagctcttc attcatataa ggtttccttc attcatccag tcaccgcaaa
    15481 acctatggaa gtttttgcat ctttacctga tgatatgaga agagctctgg aaatactgga
    15541 aaataaacat gtttagttta catataaccc caactatgtt attatacaga taaatccaat
    15601 actaataatt gtagatacac attggatata cattgaatat atcttggtca tacattaata
    15661 catagtgttt aaatgggagg taatcacatg tctattacaa aagataattt ggctaattca
    15721 aagaaaactg tggacccaaa tttggaaaac cttgctaaag agagttttac aatcactgaa
    15781 ctaagtgctc ttctaaatgt aacagatcac acactcagat actatgagaa agaattcaac
    15841 cttagaattc caagagactc aagagggaga aggtattata ctcctgaatt atctgatatc
    15901 tttttggaaa taagaaaaat gaaattaaag taattaaaga gttaatgacc aataactatt
    15961 gtttgcagcc agtggaagct aatccttccg atagcatgca aagcaatctt gatcttgata
    16021 atataaaaaa gttattgtca gatttggaga aacagcttca tatgaatatt ttgtctggaa
    16081 taaattcatc aattgattca tcttctatac atattattaa tgaaattagt aaaagcaatg
    16141 aacatttatc taaattcata gaaacaaata atacaatact tgatgcaaaa ctcgaaagac
    16201 attttcagtc tatagataat tctttatcag aatggagaaa taaaaataaa tgcggagcca
    16261 tcaagcaact gtttcaaaga attggtatct taccatatta atctaagtaa tagaatttgc
    16321 aaaataaatg atgtgaatat atagctgaaa gtatgtggtt aagggtgaaa gtatttagat
    16381 gagggtgata atgcataaag tgaactatta gaagtgggag atgagattct aaattcccgt
    16441 ctccactctc tgcttttcac ttcgtattta ttacttttaa tatcttgtac gatatgtatc
    16501 atccagcttt catttgaagt cttagtttat ctgctaccat tgcaataaac tcggaatttg
    16561 taggttttcc tttgcctaca ctgattgtat aaccaaaaat ggaattaatt gaatctattc
    16621 tccctcttcc ccatgcaact tcaattgcat gtcggattgc tctttcaacc ctgcttggag
    16681 ttgtctcgta ttcctttgca attgccggat aaagctgttt ggtgattgaa ttaataatat
    16741 ccatgtcttc tactaccatt atgattgcat ctcttaagta ttgatatcct ttaatatgtg
    16801 caggaactcc aatatcatgc ataatatttg ttacttctac ttctatatct tttttttccg
    16861 acacaatatt aaaattgtta acatttgctt tatatccttc tttttgcggt attaaagatg
    16921 ctgttccgtt taactgcttt attctggaaa caagaacatt catatcaaaa ggtttaacaa
    16981 catagtattc tgccccgaga gaaatagctt tttgagttat ccggtcctgt ccaactgcag
    17041 aaagaattat gaaagtaggt ctgtttcggg ctaacgatgc atttactttt tcgagaacac
    17101 caagtccatc gagatgaggc ataataatat caagaacaac tacatcaggt tttttctcaa
    17161 tgatttgttc atacccttcc ataccatcaa gggctatccc tactacttca atatcttcct
    17221 gagaatccaa atactcactt aaaatatcgc agaattctct gttatcatct actatcaata
    17281 ctctaatttt tttctctaac aacccaattc ccccttaaaa atggtactct tttgtaatta
    17341 ttatatttat gtttcgacaa aaatacaata ctcatttaat aattctatat tatattatat
    17401 aaaatcacat aaattaaatc tactgtaaaa tatgatggaa taatgtattt cagtggtttg
    17461 tactatataa tagatacttg caattatata taataattta caataaaaaa caggaattat
    17521 aatgggaaat acaatttccc attataattc ctgttccgta aatgtttgaa taaaattgtt
    17581 tctcgatttt ttttagacaa ttatatgagt tcgattttgt ctggagctga atttataggc
    17641 tctaagcagt ataaagtcag ttccagataa atatttcctc agtttttaaa aaatgctgcc
    17701 cacttatgaa gcattagctt ttatattctg aacaatgcta ttctcataca tttctttaat
    17761 catgctttct ataaaaattc catatcctct tgatggatca tttactaaaa cgtgggttac
    17821 agctcctact attcgtccat cctgaacaat aggacttcct gacataccct ggacaatacc
    17881 tccggttgct tctaaaagcc tttcatcggt aattttaata accataccct ttgatccctt
    17941 aacacttttc atagacacct tctgtatttc gatagaatac tcctctacct ccctgcccat
    18001 gatgttagct attatagttg caggtccttc ccttatttgt gatcttactg caataggata
    18061 agtctttcct ggtacttcac ttatggcact gccatacagt tgtccatata ttccataact
    18121 actgtttaca gtcactgaac ccagcttatc cccatcttct gcaaaaatcc ctttaagttc
    18181 tccaggaacc ccattttccc ctttttttat agctaatata ttggattcaa ttatctctcc
    18241 ccactttaca ggtattaata tcccagtatc catatcagta ataccgtgac ccaacgcacc
    18301 aaaatatgtt gtttcagggt cataaaatgt aagtgttcct atacctgccg tattatctct
    18361 gacccataat cctatatgat aattgttatc atcaatggat ttgacagggt taatctgtac
    18421 atcatgaaaa ctatcaccgc ttctataccg cagatgcatt tcctttcctt cacttgcatt
    18481 aatttcttct atcaggctgc taatatcatt tacaattttt ctattaactt gcaaaataaa
    18541 atcacccggt tttatacctg tgtcttttat aggagaaact ctatatccac ctgcagcttc
    18601 aacctctccc atcccaatta caagaatccc atcaagctta agcttaaccc ctactgtatt
    18661 gccacatgcc acaactttat tatttggtac tacattaact tttaccaatt ttaaaggaat
    18721 taccccaaat aattttatct caaggttggc acttccttta tttaatgttt taagcaatac
    18781 agaattggaa aattgtaatt ttctttttgg ttttacttgg ttccttccca gctgcactgt
    18841 ttcttcttta tcgaagttca gcttaacagg gaaaacactt tgaacattat atatatattc
    18901 ctcaccttca acgagaacta aatcgcgtgg caaagaccag gcagtataaa tacaagtcag
    18961 gaaaaggata atagcactta ttataaaata tagtccgatt tttgcaagta attttttata
    19021 tagcctcatt ctgttcctcc attgtatttt gaaaacaaat gttatacctg tatctgaatt
    19081 atagcattgc aggcaaaaca ctattcataa attaaccttc tgagaaatta attattcaag
    19141 taaaaaaatg catatgttga gtattttgag atgtgaatgc taatttttct taattttcct
    19201 taattttttt gcgttttcaa gcatttctat tgcgaggttt ttagtcatga cagttataga
    19261 atctccgcca agaattcttg ctacttcgtc tacagttttt ttatcggtaa gtttttcaac
    19321 tttagttctt gtactgcctt tttcagttat tttttcaatt aagtaatgat tatcagccat
    19381 acaggctatc tgtgcttgat gagtgacaca tatgacctgg tgtaattttg atatatagga
    19441 aagtttttct cctaccctct gggaagcctt accgcttatg cctatatcta tttcatcaaa
    19501 aatcaaagta ggtattcggt ctacatcagc aagaatggct ttaattgcca gcataattct
    19561 tgccatttct cctcctgatg caattttgga gagaggtttg agaggctctc cggcatttgt
    19621 agaaataagg aattcaacct tatccagacc attatccaaa aatttaatcc cggtaccttc
    19681 tccttgtttt tccggcattt ctatatttac cttgaaacgg acatttttca tttcaagatc
    19741 ctcaagttca ccacaaattt tattttccag aatagctgca gcctttactc ttccctgatt
    19801 aattataact gcaacaccat agagggcatc ttcagttcct gttagctgtt ttttgagttt
    19861 atttatattt tcctcacttt taataattgt atccaattct ctctcgcttt ttttacagta
    19921 ctgcaaaatc tcagatatat tatttccata tttcttcttc aacctataaa ttaaatcaag
    19981 cctttcctct atttgttcca attgtaaagg attgaattca atattatttt ttgcatttct
    20041 aatctcatcc tgtatatctt ccatttgaaa agctatttcc tcaatcttgc tgcggatact
    20101 actatacact tcatcatatt ccgaaataga ctcgagtttt gaaacggatt ctacaatctc
    20161 atcatataca gcattgtctg cccggcttcc tgaaaaaagc atattatata accattccat
    20221 tgtctctgct attttacctg catttgataa tattgtcttt tgctttatta actcttcatc
    20281 ttcaccaatt tttagttttg aattttttat ttcatcgatt tggtatttta gtaaatctat
    20341 ttttctttcc ctatcttttt catcgccaga aagtttctgc aggttgtttt ttatttcttt
    20401 tctcctatta tacaattggg aatactcatt tttagcctct tcaatttctt cccctgcaaa
    20461 tgcatctaaa aggtcaatat gattttcatt tcttaagagt gattgattat catattgccc
    20521 gtgtacatca attaatctgc tgccaatctc tttaagcatg gatactgtta ctatacgtcc
    20581 gttaaccctg caggtacttc tccccccggt agtatactct ctggatataa tgagagtacc
    20641 atcactttca ggttcaattc caaatttttc gtatatatct gctaatcttt gattatcaac
    20701 gctaaaagcg gcttcaaggg ttgccttgtc ctgtccggtt cttataaggt ctcttgaaaa
    20761 tcgttctcct aagagcatat tgatagaatc aataataatt gatttgccag cacctgtttc
    20821 ccctgttaat atattcaaac catcacccag ttctattcca acttcatcta ccagggcaat
    20881 attccgtatt tcaaggcgta atagcatttt ttcgacctcg cttaaaattc tagataaatt
    20941 ttacattaat taattctatt tactgccatt attaccatta taattcatta atcggcagtt
    21001 atgcaatagt tctatactat tatcctcaat ataatttatt taaccatctt aataaatttt
    21061 tcaactaatt tctctgctat tttttctgct ctgcaaataa tcattatagt atcatcacct
    21121 gcgatagtcc ctataatatc gggccatcta agggaatcta taaccgatgc tgcagcctgt
    21181 gccataccgg gaagtgttct gacagcaaca atattatttg cataatcact tgatacatat
    21241 gcttcattaa atacccttaa aagtttattt gagattacgc tttcgctctg tgtaactgat
    21301 gcatatctgg atttaccatc gtctgacata acttttgcca atcttagttc tttaatatct
    21361 ctcgaaatag tagcctgggt aacatccatt ccctcttgtt tcaatttgtt tacaaggtct
    21421 tcctgagttt ctatggcatt ttgctcaata atttctagaa ttttcgcgtg tctattatac
    21481 ttcatttttc tttaatctct ctcccctgaa atatatttta cttctaagta tattgtaaaa
    21541 attttttggg ttaatcctta ttagttttat tgtataagtg gatttttgta catctatgat
    21601 atctccgcct ctaacttcgt aacctttctg accatcaacc gtcaccattg ctttatgatg
    21661 gtaatcttca tcaattaacg cccttacttt cctgtcgcct gctgtgataa ttgatcttga
    21721 atacagaata tgtggacata ttgggcttac tataaccaag tcaatatccg gttctacaat
    21781 tggtcctcca gctgacagtg agtatgctgt tgaacctgta gggctggaca ctatgagtcc
    21841 atcgccggga aacatgtcca cgaaaacatc attgatgtat gtcttaacat gaagtatcct
    21901 tgacagcgca ccccttgaaa taactatatc atttaatgca gtatctttag ctataacttt
    21961 gtcatttctt attattgagg cctcaagcat cattctttct tctatagtat attttccgga
    22021 tgtcagacag tcaactgcat cttttatttc atttctttct acctctgtaa gaaagcctaa
    22081 attcccaaga tttattccga ggataggtaa acttttccta tatacttttc ttgcagcttt
    22141 aaggaaagta ccatcccctc ctagggatac aaccatttca gcctctttta atatttcctt
    22201 ttcattgtct ttctttatta tatatttgtt ttccaatccc atcatatcta caacttcgga
    22261 taggaatagg gcatatatgc ccgcattaca caagttttcc gcaagaattc tggtatatat
    22321 aagttctaca tccctgtccc tgttaactat tactccaata tttttcattg cctgcctctt
    22381 tcatatgaac cttttcagtg cttatatata ttatacataa ttattggtaa aatacaaaca
    22441 atgtttaggc ttaggcttaa agccttttat gtgactcatc tacaacctta gctatcagat
    22501 cctttatatc tgttatgtca tcttcaaaac ccggttgaac ctttttagaa acgtgtaaaa
    22561 gatattcaat attcccttca ggacctttaa tgggagaata agttagtcct ttaatacaaa
    22621 ggccggtttt taaaacaaat tctgtaatgt tttcaagtac ttcaatatgt gtatcctttt
    22681 cccgcactac accgcctttg ccaacctttt cccttcctgc ttcgaattgg ggcttaataa
    22741 ggcataccag ttctccttca gaccttatta aattaataac aggcggtatt acttttgtta
    22801 aagatatgaa tgatacatcg atagatgcaa agtccgccat tacccccaca tcttcaggtt
    22861 tcacatacct aatattggtc ctttccatgc atataacccg ttcatccttt cgtaactccc
    22921 aggcaagctg accatatcca acatcaatag caacaacctt tattgcacct cttttaagca
    22981 tacagtgggt gaatcctccg gtagatgcac ctacatcaat tgctgtcctt ccctgcaaat
    23041 ctattccaaa acaatccaaa gccttctcaa gttttaaccc tcctctgctt acataagggt
    23101 tcaggttctc ttttaccaga atttccacat cagcatcgaa ctttgatcct ggcttatctt
    23161 ctctaatacc gttgacaaag atgacccctg ccattataga atttctcgcc ttttccctgg
    23221 tttgaaaaaa acccttttca accaatagta catccagcct ttcttttgcc aattataatt
    23281 caatcctcca actttttatt ttctactttt tgcgtctata caataaaagc ttctatctgc
    23341 tcaacaagcg attcctgatc cagcccgtac tttttataca aactgtttct tgaaccctgt
    23401 gggatgggtt catctggaaa accaactgct tttactttaa tcaatggtct acctgatatt
    23461 ttatcgttac tatactcaac agctgcccta tctataagtt ccataatact gcttccaaat
    23521 cctcccgcaa tagccccgtc ctctatagtt actattttcc gggtttttat agctgaattt
    23581 aatataaggt tctcatcaag aggttttata aatctgggat tgattaattc aactgagaac
    23641 cctttcttct caagtatatc agcagcctta acagaactat caaacatatt tccaacagca
    23701 attatagtta tgtctttacc ttcctttaca agtacacctt tacctgtctc aattgaacca
    23761 aatccagata attggtactt atcattcttc aaaggtttcc agctgtcagg aacaacatcc
    23821 catatgtttt taattcctgt tccgcggggg taccttatag caataggtcc attatgctca
    23881 agaactgcat attttagcat agccgccagc tcagaataat cagcaggtgc aagtatggta
    23941 atatcaggga tatgtcttaa gtagcaaata tcatataaac cctgatgtgt ctcaccatct
    24001 tctcctgtaa tacctgccct gtcaatagcc agaaccacgt gaagtttttg caaggctaca
    24061 tcatgaagta tctgatcata tgctctctga agaaacgatg aatacacagc aaccaccggt
    24121 ctcaacccat tggaggccat acctgctgca aaggtaactg catgttgttc agcaatccct
    24181 acatcaaaaa aacgtcgtgg aaatttttca caaaatgtat ttaaacccgt accgtgaggc
    24241 atagccgctg taattgcaac aatactactg ttatcttcag ctaatttgac aagttcctgt
    24301 ccaaaaattt ctgagtaatt agggccatta tgtactttta cctcaccggt ttcaatttca
    24361 aaaggtgata ttccatgata ctcatcagga tttttttcag caaaagaata ccccctgcct
    24421 ttttgagtac atacatgaat aagtacaggt cccttcagca ttttagccct gtttagaaca
    24481 tctacaagtt ttggtatact atgtccatca atggggccaa gatatttgaa gcctaattcc
    24541 tcgaaaataa ttcccggtgt tatcatatat tttattgagt cctttgcttt gtgcaacact
    24601 ttggctgcac ttttaccaat agcagttttg ttaagcataa tatccaagtc ttcttttgct
    24661 ttaaaatata caggttcagt tcttatcttg ctcaaatagc tggaaagccc tcccacattt
    24721 ctggcaatag acatctcatt atcgtttaaa acaactatca ggtttgttgt agatctgcct
    24781 gcatcattga gtgcttcaaa agcaatacca cctgttaaag caccatctcc tataaccgaa
    24841 ataacaaagt aattttcttt ttttatatcc cttgcctctg caatgccgag ggctgctgaa
    24901 attgatgtac tgctgtgacc tgtattaaaa caatcatgca cactctcaga tgtttttggg
    24961 aatcctgcta agccgtcaag cttcctcaaa ctctcaaatt tatcttttct gcctgtaatt
    25021 atcttatgta catatgattg gtggcctaca tcccatatta tcttatctat tggtgtttta
    25081 aataccttgt gtaaagcaag tgttaattcc actataccaa gattggaagc aatatgtcct
    25141 cctgtctgcg atatgtgctc aatcaggaac tccctcaatt caactgccaa ttgttcaagt
    25201 tcgttgtaat taagaccttt tacatcatca ggagaattta ttctgtctaa caataaagcc
    25261 agtttaatta cccctcttcc tcttattctc tttattctct ttattcactt tattagcttt
    25321 attaacttta ttatctttat tatctttatt atcttttgaa aaattagttt tgctatttat
    25381 attaccacat ataaactgta atattacgcc tactttatat actatataat tactattaga
    25441 aattgcaata gtttttcctg ctgcttttcc tccaacagta aaagcagcta ctattccact
    25501 cacaagaagc ccataaatga atttttcagc agggctcttt cctgcagtta tacttacaac
    25561 aatcaaagca cttgcagtac cacttataat accgcatata tcccctatta catcgttgca
    25621 aaaacttgat actttatttg catttctgat taatttaatt gaacgttttg cgccataata
    25681 ttttcttgaa gccatagcat gaaaaggtgc ttcatctgct gctgtaactg caacaccgat
    25741 aatatcaaaa attattccag taagtatgat ggaaagaaca agaataaagg caattgtatt
    25801 gcttacatcc tggagtataa gatttgaaat agaaagaaat aatactgaca atatgaacga
    25861 tgaaactgtt attaaaatta cccaacgctt atttcctttt tttgtactta aaagattttt
    25921 cctaaacttt gcctttttat cacctggcaa tcgtttataa ctatcatcca ctaaagcctt
    25981 tcctccattt taagctgcat tcctgcggtg tgtgaaatga attcacccaa aatatgcaca
    26041 agcgcagtag ttttctagaa atttaccaat ttctttcaaa caaattcaca ggtggtagtg
    26101 tattgagtta attttgcggc ttaggtcagg caggatttcc cccagataca ctgaaatgtc
    26161 gccatttcag cggtttccca ttatatatcc ggtcgcttcg ttaccggaag caaggcctga
    26221 ttaccactta gtccacactg caatcctcaa tacacctcat atggaacagt ctaggagttt
    26281 tcctctacag agtcctccac tttctagcct cttttgcaaa ttaggtttca aacagcaata
    26341 attcaaacac atcggccaat atgtttgtat ctgatccgtt atccaccaaa cccactcaag
    26401 gcaggctacg ctgtacacag caccttgcgg taccgcataa caggtttaat cccagctcgt
    26461 agttatggtt gccgcataaa acaaaggcag ctttattccc acaagtatgg gtctccacgg
    26521 tagaggggtc aacgcccgca tgccattgca gatcgcccct gaaccttaac tcccagcatc
    26581 agcccaccac tgggcgtagc aagccgacac aaggaacttc atcgatatgc ccttaaacgg
    26641 atttttaggc ccgtcttcaa aaacagcaac tctgactaga atactgcacc acctatagca
    26701 aattcctact gttataggta atcggacatg aaggtaaaac caccaggttt tattaccttt
    26761 atggcattta ttataacata tagcaagcat ccacaaaagg atgtttaata ttcaaaatag
    26821 tttgtaataa cctgcttttt agttttatgc taatttatcg tgctgatact tattaattct
    26881 ctctttctat aatataatat gctaaacttt ttaaaaaatc agccttaggt ccaaatcttt
    26941 tcagagcctc ctcagcatca gaaattaaat catgcattat tgctttggaa ttatcgagcc
    27001 catacaaagt gacaaaagtt gattttccta tgcctgaatc tttacctgtc ttctttccca
    27061 ttgattcaaa actgccttca cggtcaagta tatcatcctt aatctggaaa gcaaggccga
    27121 tcttttcaga gtatttttcc aaatctcctt tgccttcatc atccagctcc actaaaattg
    27181 caggtaccat aacagcagcc cggatcaaag ctcctgtttt acatttatgc atatattcca
    27241 ggagttcgga agaaacctca atcccttcag attcaatatc aataacctgg cctccaatca
    27301 ttccggaaac tcctgccgct tcggctatat aactcatagc cataactacg gcttctaatc
    27361 tataacaact actcgtttta caacacaatg tactgttttt atctaagtta cttttcgatg
    27421 cattagtcag catattttcg aatgcatagt ttagaagagc atcacctgcc agaatagccc
    27481 tggcttcacc aaaaacctta tgatttgtca aacgtcccct tctgtaatca tcattgtcca
    27541 ttgcaggcaa atcgtcatga attaaagaat atgtatgtat catttctata gcacatgcat
    27601 acggcataac ctcatttaaa ttccctccca gcatttcgca cactgccaga gataaaaccg
    27661 gtctcaacct tttccctcct gccataaggc tgtaattcat ggccttataa atactctttt
    27721 ccggcgtttc ctttttaact ataatttctg acaagctgct atttatctga tctaaaagag
    27781 aattatactt tatcgtaaac tgcgttttgt taaaagacat cagcatcctc cgggtcttta
    27841 gaaagttgta ccccaccgtc aatattaaaa ttttcctctc ttacatcccc attttcatta
    27901 tctataagta tggttatcct tttctcaatt tcatcaagtt ttttactgca atatcttgaa
    27961 agctcaatac ctttctggaa atattcgata gagtcgtcaa gaggcaattc acctttttcc
    28021 agtttctgca ctatttcttc caactcattc atagattctt caaaggtttt attctcttct
    28081 ttagttttat tctcttcttt cattgtttcc tcctttattg tcccctcttg atatattatc
    28141 tacaattgcc tcaactctgc catcacttac acttatctca agtctgtctc cactatcaac
    28201 atctttcact gattttaaca gcacattatt attcttaatc ttaaccatac tataacctct
    28261 agctaaaacg ttcaatgggc ttaatgaatt aagtttgtca attaaaaaca acatccgcat
    28321 cttggattta tctttttggt ttaatacggt acttactaat tttctttcca gattgtcaag
    28381 tctgattttc tcctggttca gtctattata cggctgttta aataccatgc ttcctgtaat
    28441 tcttaacaaa tgaaccttgt taaaattaat gttttttaga agggcatttt gcattcgcat
    28501 tcttaaatca gcaattttct gttttaaaag ccttttttcc ggaacaatta tttctgcagc
    28561 agctgaaggt gttggtgctc gtaaatctga aacaaaatca gatatggtaa aatcagtttc
    28621 atgacctacc gcagatacta caggtatcct ggaattaaaa atgcttcttg ccacaatttc
    28681 ctcattaaaa ggccatattt cttcaaggga tcctccccct cttgcaacaa ttataacatc
    28741 aacacaatta agttcgttta gccgtcttat tgcccttgaa attgcagaag cagcctggat
    28801 accctgtaca gccacaggat atagtttaat attaacattt ggaaatcttc tgctggcaac
    28861 atttataata tcttttatga ccgaacctgt tgaagatgtt actacgccga ttgactgggg
    28921 caggtaagga agttccttct taaacctgtt atcaaacaat ccttcaaact ccagtttttt
    28981 cttgagctgt tcaaaggcaa gataaagatt acctatgcca tcaggctgca tttcctcaac
    29041 atatagttga tattgcccat cccgttcaaa tactgagata tagcctcttg ctattacttt
    29101 cattccgtta tctggattaa acttcaaacc ataagtctgg cccttaaaca ttacgcattt
    29161 tattaaactt ttctcatcct taagggtaaa atacatgtga ccggaataat gattcttgta
    29221 atttgatatc tcccctttaa tccatatatt ggacaaaatc atatctccgg ctataacctc
    29281 ttttatatat ctattcactt ccgttacaga caaaatatac tgcatagttc tccatctttc
    29341 ttgataccat ttatttctga atttctatat ttcatttcta tatttcattt ctatatttca
    29401 tttctatatt tcatttctat gtttcatttc tatgtttcat ttctatgttt caggtgtaga
    29461 aatgatttcc gttttcggta ttttgctcaa tatcccatta ataaatgctc ctgcctcact
    29521 ggtactatac ttctttgcca tttctacagc ttcatttatt gttacattat atggaatatc
    29581 ctctctaaaa agtatttcat atatacttac tcttaatatt gaaaggtcta cctttgaaat
    29641 cctgtttatt ttccaaccta tagcatattt ttcaataagc tcatcaatct tttcagtatt
    29701 atcaagcaca cctttaacaa catcttcaat ataaatttta tccttcttag tgaatgaagg
    29761 ttgctccaat gataatctta tctgttcttc tctgtcatcc ttctgtattt caacctgata
    29821 taccagcttc atagcagctt ctctggataa tttacgtccc atattttaca gtatatacct
    29881 cctgctttag tttattaaaa tttatttgta cattcctgga gggaatatct tatcttttaa
    29941 gttctttaaa aaatgtctgt cgtcacttat gctcttccct atataataac ctatgccaac
    30001 acataaggca ataaaaaaca cttttataaa accaaaaata aggatagtta tagctgctac
    30061 aagtcctata gctgcaccgt taattgctcc ctggtgggca taatagtatt ctatcaaact
    30121 tctccaatcc atgccaattc ctccaatcag attttttaag tattatgacc ccttttatca
    30181 gtagaacgaa ttgctctctc tcctacgcaa ataaatatta ttacgattta ttcgattttt
    30241 catccctaac ctctggcttc tcaccactta cccctggttt cccacctcta gcctctaatt
    30301 ttacacctgc cgcagccggt agatgtcctg aatatatgtt ctgcacgaac atccttacat
    30361 cttctaccaa tattcctgta ctttcttcaa ctgcaaattt tacttcttcc tggattttcc
    30421 cggaaagagc aggtatgcaa acatcaggta ataccgacgc cctaacagtt atggaaacat
    30481 tatcagctac ctttcgtatt aaggtgctga tatcccttat ttctccaaat ttacccgctg
    30541 tattcagtac tatatcatct attgtagcaa gggatatttt tacttcaccc atgttagtat
    30601 atttgcttac tgatttttta tccctgttta attttacacc cgatattaaa gtaactatac
    30661 ctataaataa aaaagcaaga gaaaacagaa taataaccag tgttgagtag ctgtttgttt
    30721 caataagcgt acttaaataa cggtatacat caaaaagaac atcatgtctt agcgcaatta
    30781 tcattgaaag tgatgataca aaaataaaaa taacagcaca caggactagt aaaattcttg
    30841 taaatatatt catatgtata catgcctcct tttgggtttt gtattttggt tttgtatttt
    30901 ggttttgtat taaattctct tttacttaaa ttatagttta ttcactcaaa aaaataaacc
    30961 ccaaaggggg ttattttaat ttttttagtc agggtagcag gatacacctg tagtatttat
    31021 tttgcctggc tttctgcaat attacaaatc gtcttgctca atctgttctc catcaggttt
    31081 aatactcatt tcttttttat tttcttttat tatattaatg ccctgtacat gaatgttcac
    31141 ttcggtaacg acaaggcctg tcatagattc tacagttttc ttcacgtttt cttggatatt
    31201 ccaggctacc tccggaattc ttgctccata ctcaacaata ataaaaagat taataatcgc
    31261 ttctttttca gttacttcaa ctttaacgcc cttggacaag tttttcatac caagagcctc
    31321 agcaatgcct ccggcaaaac ctccgctcat actggcaaca ccctgaactt caattgcagc
    31381 aagtcctgca ataacggaaa caacctcatc ggtaattttt accgaaccta tttcaccctg
    31441 tatattggta tttatctcct ccataacagt accccctttt ctgtattatg tataacataa
    31501 aaattatcca acttgttacc accaattata gtacttatgt ttacacaaat caatctgaaa
    31561 ttgcttattg ggtgcattta gtttaagtga agaaagtatt tggtaacgac agctcctggt
    31621 aaatatttct tcacttttag taaatgcacc cttgcttttt tattctaata tttgcttttt
    31681 acgcttatca tatctatttc aatgtttgca tgtctggtaa caatatctgc aatctggacc
    31741 acctgggttg aggaaagatt tggagcttta acaataatat caacacttcc gtcatcggca
    31801 aaaaaagcaa taacatcact aaacccctgc tttttaataa gagcctcgat cctcatttct
    31861 ttatctgagt tatcaattat agccatcatc tttgtaaagg cttgatcttt aatttcctga
    31921 cttataccct catcagcagc tatacttccc agtttatccg tttccttgct tctgcttatt
    31981 tccttatcaa gttttgtttg agcaaagtaa ttttcggctt cttttgaagc agcaaccgta
    32041 ttttcggttt tttctacaat atgtttttct gatgtttcct tttgtgctaa ttcatcttca
    32101 tttccaactt ttgtactagc tgccttatcc cctgtatctt gaagcaaatc cgggttatca
    32161 acataaactg cttctcctaa ttttccggta tcatcatctg cttttgaaaa cccgcttttc
    32221 ttataactat actgtacata acccgctacg actatcataa gaacaagcga taaagctaca
    32281 atttgcctct tttttaaaat tgtcaccata aatacctccc ctttgcacct gtcaccactt
    32341 gattttcaac ttatactttt ctttattctt atgtatattc aaataaaaca aatttatatc
    32401 tattctaaaa aaattttgtc caacttatta ccagtactaa ttatttttag cactcttggc
    32461 tctggcaaaa acctgaactt tatggggggc tatatccacc agcgtttgta cagctcttac
    32521 caggttttcc ttgactacag gctcccctgc gccatcggca acaaccacca cacccctcac
    32581 ttttggttcc atctctttta aaattatagg tcgtttaact cctccaggct gttcctcata
    32641 tgcaaccctg ctatccgatt cattttgtga tatatttctt ttcccacctc tgtcatcttc
    32701 ttcctgagta ctattatgac tctcccgttt atcatatgcc ggcacctttt cgctgcctga
    32761 ttcatatgta attgcaacac ttactttgcc agcaccttca atttgtgaga gaatttcttc
    32821 tacctctttt tcaatactgt agactcctcc tgattttgat gtcagtattc cttcctcaga
    32881 attgttcaca ctttccatac tggatttctg aaaattccct tgtcctgtat ttccaataat
    32941 attagtattc ttgcttttaa atagagtact accagcgatt agaaaaataa ttccaattat
    33001 aattatgata atactgttct cgattatttt tttcttatcc tgacccgcta taatattctt
    33061 cactccatca catatttttc taactaactc catacgccta ccccatttct tttttattat
    33121 gtatttaagt gtaattttag tatcttaatt ctattattgt actgaaatga taatgttttc
    33181 tatatctata ctaaattctc cggctatttt tccttgaagc tttttaacaa gctccacatt
    33241 taaatcatta atgacactct tttcatcctc tataacagtt tcatctcctt cccggatttt
    33301 tatatccctg attttttcaa tgttaacatc aggagctatc ctgttttgag gtttatcttt
    33361 attttttagc tttaaataaa cataaacata gatttttttt atctctccat aatattggga
    33421 attgtagtct tcatttacaa taacttctga tgctgcttcc tttactccat caatctcttc
    33481 cgccaattcc actatccttt cattaactct ttgcatgtat atgctcagaa tttgcgctga
    33541 ttgtttttct ttcagcttct tactgtcctt atctgcggat acctgtgcta tggcagagat
    33601 ataactattg ttgcggttga acagttcatc caaatcaata ttactgaaaa ccttactaaa
    33661 aaaaggctga actattgtta atatcagaat aaaccctgat acaaggttta caaatttttt
    33721 tgttttaccc gaaggaagca gtatctccac aagtattaaa agaatgctta cgactgttac
    33781 acttaagacc cattctttta gaaattcaat catttcaatt tacccactcc attcaagatc
    33841 aaatttaact ctgcaaatca gctgctaatt ctgctaatta ttcaactgta gataacgcac
    33901 aaaagaaaat gcataaccaa aattatttta cctgaccatg gctgtcatat ttccagctgc
    33961 aattattaca gttatggaaa taacaaacat aaaagcaaca actgctactg ccccaagaat
    34021 aagtgtaata gaatttgcca catcatttat acaattggta atacgttttt cagatattgg
    34081 ttcagtcaat gcacaggtta ttttatatag tgatattatt acggacattt ttaaaaccgg
    34141 gataagacat atcgaaatta ctcctagcat taccaaaact ccagctgcat tttttattaa
    34201 aagagaacag ccaatcacca tatccgctgc atcagataga tacccaccta caactggtat
    34261 aaaagtccct atggcaaact ttatagtttt attaacaaca ccatctgcca cagcgctaac
    34321 aggtccctga atggtaacca cacctataaa aatcgttagg ataattccca aaaaggccat
    34381 cccgattttt ttaataagac cggctaatct ttccagatga agtttttcgg atacattatt
    34441 tataattgat agtgcagtcg aaagaaatac aataggtata aatacatttt ttataatagt
    34501 ggctgcagtc tcaacagcca ttataagtat tggctggaat actcctcctg atgttatgct
    34561 tccactggat ataaggagag ttaccattac aggaatggta gcatacatga accctgccat
    34621 ttgttctata acattaattc caaaagaggc tgcttcttta aagcctataa caagcatgga
    34681 aactataact atatagcata caaaaaacgc cagttctcct acaccttctt taataaacga
    34741 attctgaagg ttattcaaaa ctgcacatat aattataata gttattaatt tcagcagtat
    34801 attaagattc aagtatattt cctgtataaa ataggataat atttttttag tcaatttgct
    34861 tatattaagt ttcaaatcac ctttaacggc atctttaatt atttcatcgg ggtttaattc
    34921 gtccagcagt ttaccccctg cacctgttgc atactcactt aagtattctt ttaaagcctt
    34981 tatttcggaa gactgtaatt gattccggat cagctcgtcc cgagcttttt caattgcttc
    35041 cgtactgtca tatttacttt cctgtgcaaa tgtttttttt gagcaagaaa tctggaatac
    35101 cattaaaata gcaattatga gaaatgcttt ttctgttatt ttaattaata ttgatgtttt
    35161 gtttcttttg ttttttatct tcatttttta ccacccaagc atattattaa ggcataattc
    35221 ttattattaa ctcaagtaaa gttgtaacaa tgggtactgc cagcatggca attattatct
    35281 tgcctgccag ttcaactttc gaagctattc cattttctcc tgcatccctg cacacttctg
    35341 cgccaaattc ggttatatag gcaataccta taatctttat tagcacaggc aggtattgta
    35401 tatttagatt agctttgcct gaatatattc ttataaagtc aaatataggg gcaattttac
    35461 ttactattag agcgaatatg attataccag ccattaaact tgcctgaact gctatttcag
    35521 gtctatgggc cctcaacaat acaataataa tagtagaaag caaactgaat ataaccacct
    35581 gtattatttc catactgcaa tcatccgcct tagccttaaa attgaaacat ggtttttata
    35641 agattgaata aatttacaat ttctttggct accatcaaca ataccaaaat caaccctgaa
    35701 agagtggtaa gcatggcaag ctcatccctg cctgattgtt ttaataccgc atttaaaatc
    35761 gaaactaaaa tacccactgc tgctatttta aaaattacat caatatccat aattcatatt
    35821 acaccctttc cctcaagtat ttaagtcaat ggtatttaca ctaaatcaac agtataactg
    35881 ctgtagcacc acctaatatt ccaagagttc tatacattgt ttcatttctt ttcttcaatt
    35941 cctctgcttt ttgctcctgc agacttaact gatatatagt aaatcttata ttttttattt
    36001 gtccctcaat gtccgaattc ccaagcattt tcccaaaaga cactagtatt tcttcgtcct
    36061 ctttgtttag cgaagttttg tatatattat cttttatggc tctttcccag gcctgagcca
    36121 gatttaactc ttctctgttt tcaaggtatt caagtgttga cttgaaaaac aggtttaccg
    36181 gattatcaat tgacttgtaa attttctcaa aagcatcaga aattatactg gatagatatc
    36241 tgatctcgtt ttctagcatc tgcattaaat tctgtaaatt tttcagctga ccaggtcttt
    36301 ttgagcaaca acttgacatg gcaaatccaa taaaacttga cccgctcatt atcattatgc
    36361 ttccaattat tttcagcagc atatacagtc tctcctttat ttcttttgtg cagcgcctta
    36421 tcaatacctg cataatgacc gcatattcag acccgggtaa tacctgcata ttcacgtttg
    36481 tataatactt tcatattcga gccgtctatt acttcttcca tagtccccgg tccttgtgta
    36541 ttactcaaaa cgatatatct ttcaaagact ttttcattaa tcatggacaa aacctcctgc
    36601 ctgcttttaa gctctgatat attatatcca tgagctgttg ctataagctt gatccctgcg
    36661 tttaatattg caataattga ttccttatct cccttatttc ctatctcatc tgtaataatt
    36721 acctgtgggg acattgaacg caccattaac agcatacctt ccgactttgg gcatgcgtca
    36781 agtacatctg tccttatgcc taaatcaaat tgtggtatcc ctttgtaaca ggctgcaatt
    36841 tctgaacgtt catctattaa ccctaccttc atacccttaa tacctttatc tattgctcca
    36901 tcacttaaaa ctctggacag atcacggagt atagttgttt ttccacattg aggaggtgaa
    36961 acaatcaatg tatttaaaac ctgtttattt tcattaaata tgtatttcag tattttaaat
    37021 gagcaccctc gtacttcttt tgatatcctt atattaagac ttgaaaactc ctttatattt
    37081 accactttgc cactgtcagt tattactttg cctgatatac ctaccctatg cccgcctgac
    37141 aagctaataa agccattttt tatatcttcc tggaaggcat atatagagtt tttgctcatt
    37201 atttccaagg tgcttagaat ttcttcccgt tttacgataa aagcatctct aatatttttt
    37261 gtaagatttc ctaagctatc aacaaaccat tcttcagaat aattctgtat aattaaaggc
    37321 ttatttgctc tgagccttat ctcttccaga tcaaacaact tatccggttt cactttttcc
    37381 aatatttctc ttacacctct ggaaaggtat tggattatat taatattttt atcttttgca
    37441 gacaactctt ctcctccttg tccttttatc cgttttttgt tttctgtctt ctactctcag
    37501 ttctctgttt ttactttttg ttttctgttc cctatataga tatttatctt tgtccaaatt
    37561 tatgacataa aaaaaacagg tcgtccaaaa tatatggata acccgttttt aatgaagtga
    37621 gagaactcat ttctctcgag caaaagtatt ccttttacgt tgagcaatgt cccttatgtt
    37681 aaatttaatt acttatcttc atcattttct tcataatcgc tgcaatcttc acattcttct
    37741 tcacaatctt cacattcaca tacccattca atatctattt ttttatgaca gttagggcat
    37801 tcgatggtct taccttcatc atcgaacatt tcctttttaa gtacaactac attatagcag
    37861 taagggcact ctaccataca taggtcatgt tcattgcctt catcagattc aaaagaatgg
    37921 tcttgttcgt gttcatgatc gcagccacaa ctgcaggtat tataattata atcataggac
    37981 tcgtcatcgt catcattttc atcgtcttca taatcaaaaa tcatactttc aatttcggcc
    38041 agatcctgat caagatcttc tatctgttcg ccttgctgat cctgagtttc ctcaatatca
    38101 tcaactgcca gggcaatgtc atccagaaca tcaattattg cccttaaaag cttaccctca
    38161 tttgaagttt cacttatctg cataccttca gcaaggcctt ttacgtaggc tacccgttct
    38221 ctcaaataat tcattacata cctccatacc aatattacac tttaggttca tatgttttca
    38281 ctatattttg ccactttttt caaactttat acccttattt cctatgctct ttccatatat
    38341 tcccctgttc tagtatctat tcttataaca tcaccctggt taacaaataa aggtacctta
    38401 acgacagcac ctgtttcgac agtagcaggt tttgtagctc ctgttactgt atctcctcta
    38461 gccccgggtt ctgtgtgaat aatttcaagc tctacaaaag tcggaggttc tactccaaac
    38521 acattgctat tatgagacag gaattttaca gtcatgcttt ccttaaggaa tttgagcgca
    38581 tctccaagag catcctttgt taaaggaacc tgttcataag tttctacatc cataaagtaa
    38641 taaagctcac catcattata aaggtattgc atgtctttcc gctcaatatg ggccttgggc
    38701 atcttgtctg ttgggttaaa agtcttttca accgttccgc ctgtaataac atttctcagt
    38761 tttgtcctta caaaagcagc ccccttaccc ggttttacat gctgaaattc tataacctgc
    38821 ataacttgtc catccatttc aaaggttaca ccatttctaa attcacctgc tgaaatcatt
    38881 aattatccct ccaattcata tataaaacta ccaaactaat aaaagttaaa attgaactct
    38941 gcacactatt tttcaaactt acagcatcaa tttttactcc tgttcatttg caatatggcc
    39001 gctatatata atctatgtat aatgtataaa agctttttca ttatacatag attatattaa
    39061 aataaaatct gtaacattgc aagaaatatt tttgttttgc ttcatataca acgcaaaaat
    39121 gattctatag ggaacaaatt ggatttttgc gtattcgcta aggtaatata agcgcacaat
    39181 aattatatac gtattttctt ttgtgcgtta tctatagttg tcttatattc atactatagt
    39241 caatttcttt gaagcctttg tcaaaattaa cggctcatcg tcatttataa ctatcatatc
    39301 ctcaattctt acgccaccaa atccttctat ataaataccc ggttctactg taacaaccat
    39361 gccattttcc ataattttat cacccgcagg tgcaaatctc ggttcttcat gaatttctaa
    39421 tcctacacca tgtccaagtc catggccgaa attatccccg taaccagaac gggtgattac
    39481 ttctctcgca atttcatcta tctcccggcc ggttttccct ttaaaagctc catttaatgc
    39541 ctcaatttgt gcctgcaata caatctcata caccttctta attttgctat ctgcatcacc
    39601 caggaagaca gtccttgtaa tgtctgaaca atatccatta tatattgcac cataatccat
    39661 tactataacg tcgccatact caagctcctt ttccgatgca acaccatggg gcattgcaga
    39721 tctgacaccc gaagcaacga ttgtttgaaa tgaaggtccc tctccaccct gcctcttcat
    39781 aaaatattcc atttcagcag cgatctcagt ttctccaact ccgggtttta tatagctcaa
    39841 aatgtgatta aaagcctcat ctgcaatctc tacagccttt tttatatatc caagttctct
    39901 attgtccttt attatacgaa tggactcaac aatatcgttt aacggtacaa gttcaattgt
    39961 ctgaaatttt tccttaaact ccacatattt attgtatttt acatcagcag cttcaaaacc
    40021 cagcttgctt actccaaaac tttttagcag attatttatt tctaaagcta tgtccccctt
    40081 atatcttaca atttgaaaaa gcggagcctg gctggaagcc tgttctacat atctaaaatc
    40141 cgttaccagt actgcatcct ttttagttat taccagatgt gccgaagttc cggtaaagcc
    40201 tgaaagataa atatagtttt ccctttttgt gattaaaatt gcatcaattt tattttcgtc
    40261 caatttttct cttattctgg caagacgttt ttcggtgtac ctgtcgccat ccgaattttg
    40321 tctatttacg ttattttgct ctttcataat atataatacc ctccaaagat tcatgttttt
    40381 gtcttacaac aatcttacaa cagtcctaaa gcaattctac agctgcataa agcgctagaa
    40441 tgtaacttcc cttgccgaac ccgcatattt gacctgcaca tacaggagca ataacagacc
    40501 tggatctgaa ttcctctcta gcatgtatat ttgaaagatg aatttcaata gcaggtatat
    40561 ctacagattt tatggcatct cttatagcta tgctataatg tgtgtatgca ccagggttta
    40621 ttactattat atcatataca ccccttgctt gatgtatcat atcaataata tcgccctcat
    40681 ggttggattg agtaaactct atttccacac ccagattttt tgccttttct gatacataca
    40741 aagctatatc ttcaagggta gaatttccat aaacactctt ttcccggata ccaagcatat
    40801 taagatttgg tccgttaata aacaatattt ttttcatata tcaacctccc ataaactata
    40861 ctactatgcc aatttggaaa gcgatgtcca ttaacaacat aatgaagtag tactaaattc
    40921 ctataagtaa atatctacaa tatcacattt ggcatatttt agagtaaatt tcctttagtt
    40981 ttagagcatc ctctgccata tttccccttg attcacatat gattacaggc tccatgtttt
    41041 tctttttgat tgctattgcc aaatactcaa actcaggccc aaattccgtt tctgatagtg
    41101 tacggtgcct cttttccccg ccggctgtga attcaattct gctgaaatgg atgtgaatat
    41161 gcttgagcct ctcatttcca agtgcatcct ctacacgatt taataccctg tcaaaatctt
    41221 cagttgaatt aagatttcct ccatgtcttg catgcagatg accaaaatcc agtgttggga
    41281 taagtctttc atctatagca cagatttcaa gtatttcatc aagagatcca agctggttca
    41341 ttttacctaa aacttcaggg catacattta tatttccata ccccatgtca tctgagcgag
    41401 caatagcttc cctcatagcc tcaatagaag tatccagcgc ccatcttcta tcaactttgc
    41461 tgcaggaacc tgtatgtact actattcttc ctgcgcccat ccaacttgct gcttccaggg
    41521 ttttaataat atactgtatg gatttttcac gcttttccgg ttcatcactt gccaggttta
    41581 tgtaataggg agcatgaatg ctaagaaaaa tattattctc cctcgcccta tctcccaaat
    41641 cttctgcggt ttttctgcca atattgatgc ctttgctgca tgaatactca tatgcattca
    41701 agcccatatc tttcagccac ccgggcattt gtaccgatga tttgaagcct tcgtcataaa
    41761 agctttgtga attacctgat ggtccaaatc taatcatttc ttttatctta ataatctccc
    41821 ttcaataata ctaatcctaa agccatgaaa agtaccattc ccatatatta ttaccaaata
    41881 gtatcgtaat aaatactgct attgcaataa atgggccgaa cgggattaca tcctttcttt
    41941 tttttatcct aaataatatg agcaaaatac ttgaaaatcc accaagaaaa attgacacaa
    42001 ccagtgtcag aagacacatc ttccacccaa gaaaaattcc tattggtgca aatattttaa
    42061 catcgcccat acccattact tcatcagact tatatataat catccccaaa atcgctatta
    42121 gaaataaaac tcctgaaccc ggcagcaatc caagtaacgg ctcccaccat tttttactgc
    42181 cgtaaatact taagggcaga aatatattgt atgtaaatac cgccaaccct cctataagtc
    42241 cagcaactac tagttcattt ggaatagttt tagttttcag gtcaataaaa aacataataa
    42301 taagaataat catcaggtat gcaaaaaaga aaaaatctgc tgttatactg tatttggaat
    42361 ataatatgac aaagactact cctgttacaa tctccagcaa caaacttttt ggagagtaag
    42421 ttaaacattt aaattcaata gtactgcttt tttcatgaaa ccaatttttt ccaaaaggtt
    42481 tctcttctga aaggttttta attatcacat gattaaacca accgaagaaa atgcccacaa
    42541 aaaatactat tatatacttt ataaaattaa tcacgccacc catacctcat ctattctttt
    42601 aatacaatct ataattttaa tccaaaaact gagatgcatt tatcacagat attccctgaa
    42661 agcttcaaac aagcttttaa ctatttcccc aggtatatcc atttccatcc atatctcata
    42721 agcttttatt ccttgataaa taagcattcc cagtccatta acagctttac agccctgctt
    42781 ttcagcttcc tgtaaaagcc ttgtcttttt ggggttatat ataacatcgt atattatttg
    42841 gttagtggaa aagtttaatg gtatagttaa aggaagtttg tttatatccg ggtacattcc
    42901 taaagaagta gtattaataa ttatatccgc ttgttttatt gcttcagata tattattatc
    42961 ctgtaaatca taatagcctg cgactggcaa gatattctta ttgataatat ctgctatttc
    43021 agctgccctg gaaattgtac gatttataat agaaattctt cctgcacctt caaaggctat
    43081 tcctgtggca attgctcttg cagctccacc tgccccaatt atcacaacat tcttatcttt
    43141 aaatcctgtt cctgcttctt ctttaaatga attgacgaat cctgctgcat cagtgttata
    43201 cccatataac cttccgtcaa tgtttttcac agtgttaact gctcctatta gagaagcttc
    43261 tctagataca ctatctatat aatttataac atcattctta tagggtatgg ttacattaaa
    43321 gcccaaaaca tccaatgctt taaggccttt gactgcatac tccagataac cttcaccaac
    43381 tctgaacggc acataaacag catcaatcct cagacagcag cttaatgtgt tatggagctg
    43441 cggagaaatc gaatgggata acgggttccc aattacccct aataatttgg ttttaccggt
    43501 tacactatgt acaatattca taatcttagg gcgtggagac tcgcagcttt agtcatgtaa
    43561 ggaaacgccc ctcctctttt tatcttttaa tggtagttta tacaatagat atagttagtt
    43621 ctattactgc ttactgctac ttactgctgc ttactgtcta ctatctactg tttacttttt
    43681 actgcctgaa ccagcctgtc caacggtttt ataataccta ttcagtatat acctttcggg
    43741 atatcttttc aacattacaa ataatgtttc ctttctatta ataggcttaa caagtattgt
    43801 atacataccc agcctgttcc caccataaac atcagtaaaa atctggtctc caacaactgc
    43861 agtttcagcc ggcttcaatc ccaaaacctt cagggctttt ttaaaagagc gccgcccggg
    43921 tttcgaagcc ctatatattg cttccagaca caatttctcg ttaaacctgt ccactctttt
    43981 ttttgtagca tttgatacga tacatacttt aaagcccctg ttttttagac gttctatcca
    44041 ttcaaggaca ttattatcag cttctttaac aaatgaaggt acaagggtat tatcaatatc
    44101 aagtatcaaa cccttgatcc tttttttttg aagcatgtcc aaatttatat cttgaaactt
    44161 gtccactatt aaacagggaa agaatttttc tgtcatcagt aacctccagt tttctttatt
    44221 ataagctaaa cgctgcgata tgaatataat agcaaatcta atttttaata tcaattacca
    44281 atatagccaa ttttattaca ccagaaaaac agtatgcttt aaatgtaaac attcttcaaa
    44341 acaccagcaa ctatactatc cttttccttc cctgataaca gcaaatcact tttaggatca
    44401 aaagaagctg tttttctacg tccctcatca tattcaaccc taacatcacc atatatactt
    44461 gatgtacaat catttttata ataataaaat ttaaagcaac atagcttttc tcttgttaat
    44521 tcgtaaacat gaaaatacaa ttccataaat attcccccaa taaataagca tactactatg
    44581 ccaacttgaa aagcgatgtc ttctggccag aaatacgctt tccataggca acataacaaa
    44641 gtagtaatat attaatatgt ttatgctgtt tgtactaata agagaacaaa atcttagctg
    44701 cataatatat atgtttttgt ctcatacctg taaatttttt atgttttctg tatatttata
    44761 catttacatt ttaattcctt tgatgtatta tttgtagtat atttttatta ttttgatatt
    44821 gagcggatag caataacaac taaattttaa tatttgcatt gaaaggagca caatatggta
    44881 aatcaaatta gaatcgaaaa aacaaaatca ccgaaaaata aacccaatca aagcaagctg
    44941 ggttttggaa aatactttac agaccatatg tttgttatgg agtattcaac tgaaaaaggc
    45001 tggtatgacc ccaaaatcgt accttacggc ccattggcac ttgacccttc cacaatggtt
    45061 tttcattatg gtcaggctgt gtttgaaggt ttaaaagcat atagggccaa agacggaagg
    45121 atcttgcttt tcaggccttt taaaaatatg gagagaatca atatttcgaa tgaaagatta
    45181 tgcatacctc ccattgatgt tgattttatc gtagacacaa tcaaaacact tgttaagctg
    45241 gaagaggact ggattcctac tgaagaaggt acatcccttt atataaggcc atttattatt
    45301 tccacagacc aatacctcgg tgtaaaacca tcaagtacct ataaattcat tgtaatacta
    45361 tctcctgtag gagcttatta tccagaagga ataaatcctg ttaaaatata tattgaagat
    45421 atgtatgtcc gtgcagtaaa aggtggtatc ggttatgcta aaactcccgg aaattatgca
    45481 gccagtttaa aagctcaggt tgatgcatct aaaaaaggat atacccaggt gctatggctt
    45541 gacggagttg ataaaaaata tattgaagaa gttggcacaa tgaatgtaat gtttaaaata
    45601 aatggggagg ttgttacacc ctctttagaa ggaagtatac ttgcaggtgt tacaagggat
    45661 tcagcaatac aactgcttaa gagctggaat ataaaggttg ctgaaagaaa aatcagtatt
    45721 caggaaatat atgatgccca tgcagcagga accctcgaag aagcttttgg aacaggtact
    45781 gcagcagtaa tttctccaat tggagaactt aactgggatg gtaaaataat tactataaat
    45841 aacggtgtta ctggtgaaac cgctcaaaca ttatatgatg gcataacagg aatacaaagc
    45901 ggtaaaatag aagatgcttt tggttggact gttgaggtta aataataatt ataaaatatg
    45961 gcaaaataca attatccaag tgcataaggt tgctaaagtg aatattatac atggaaatcg
    46021 tttcttgcca tattttttct tcaaatattt ccataaaata ataaagttag taatatgttc
    46081 atataataat acggaataaa ctttatggcg gttaatatgg gtaatcgtat ggtaagtatt
    46141 aggctaaaac agaaggctgc taaatctaag tgcaagatag aatctattaa agcatttata
    46201 ttgccaataa taagtatagc ctttattctt ttactgataa tattctcaaa aacagctgtg
    46261 agttcagcac taaaaggtat tgatttatgg ttgaatgtag tatttccttc tttattcccc
    46321 tttttgacag cttctgaatt gctgaatggt acaagttttg taaaagcggc aggtatattg
    46381 cttgaacctg taatgagacc attgtttaat gttccgggat gtgcttcttt tcctttcgca
    46441 acaggtatga ccagcgggta ccctgttggt gctaaagtag ttgcatctat gaaagaaaat
    46501 aagctgctaa ataaatggga tgcagaaaag ttacttgctt tttgcaataa ctcaagcccc
    46561 ctatttataa taggtgctgt ttcaacaggc atgctaaaaa tgccgcaaat aggtccgttg
    46621 ttgcttattt gccatttagc tgcaggaata accgtaggtt ttatatacag gtttattcca
    46681 gtaaacccca aaaatgtaac tctttcaggt ggtagaaagc ctgtatctgg taaatctggt
    46741 agtacaagca atattgattt tgggagctta ttgggaaatg ctgttagaaa ttctgttacg
    46801 acactccttg taattggggg atttataatt ctattttcgg ttattattgg tattttaatg
    46861 gaatcaggta ttctaaacag ctttgctggt ttgttataca ctcttttgaa atttaccggt
    46921 cttccaaaag aattgtacat atctctgatc agtggattta tagaaataac aacaggtttg
    46981 ggaatgcttt gcagctcgcc aaatataacc cttaatatga aattaataac agtaagtgca
    47041 ataataggct gggggggtat ttcagtacat atgcaggtca tgagtgttgt tcgcaatgca
    47101 ggtttaagta taaaaccata cctgatgggc aaatgtgttc aaagcatact gtcagcaatt
    47161 tacactatgg gaggtttaaa attaacaggg ttatatccag aaactgctgc ccttgtattt
    47221 aagaactttt acactccaaa tctcagaacc tggaaccagt atctcttaga a
//
LOCUS       DAOSDD010000005        41848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_525_length_41848_cov_5.316799, whole genome shotgun sequence.
ACCESSION   DAOSDD010000005 DAOSDD010000000
VERSION     DAOSDD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 41848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41848
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_41848_cov_5.316799"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..2276
                     /locus_tag="PK733_00850"
     CDS             144..2276
                     /locus_tag="PK733_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOJ09127.1"
                     /translation="MEYLSGIVERITFSNEENGFSVIKIKSKGFADLVTVVGSLAAVN
                     VGATVKLKGEWKYDSKYGKQFSAIEYQETVPATIAGIEKYLGSGLIKGVGPVYARKIV
                     RKFKEDTIRIIDEESDRLTEVDGIGTKRLAMIKKAWQEQKEIKNVMLFLQSNGVSTAY
                     AVKIYKTYGNESIEIVKNNPYRLADDIWGIGFKTADKIARQLGFDKNSYERCRSGIIY
                     VLNELSNDGHCYAKREQLIDEAEKMLELEKELIDSTIDKMISEKTIIMDAEDCIYLPP
                     FYFSETGTAKRIKEILSVESVYKNFDTDKIIKQVQEECDIEYDKVQVEAIKTAAKSKF
                     MVLTGGPGTGKTTTTLAIIKVFQKMNAKVLLAAPTGRAAKRLSETTSMEAKTIHRLLE
                     YKPPEGYKKNEESPLDCDVLIIDETSMVDIILMYNLLKAVPVQAVLILVGDVDQLPSV
                     GAGNALKDIINSGVVDVVCLTRIFRQALGSAIITNAHRINKGEFPVLRGGRDNDFFFI
                     EEEDPQKIVEIIKELCAKRLPGYYKADPINDIQVLSPMQRGETGAFNLNQVLQETLNP
                     SEICIKYGGTTYRLNDKVMQIKNNYDKNVFNGDIGLITSIDMEDKTLVITFDGNDVEY
                     DVTELDEVLLAYATTVHKSQGSEYKIVVAPLTTQHYMMLQRNLVYTCVTRAKKVLVLV
                     GTKKAIGIAVSNDKTQKRNTMLEVRLKN"
     gene            2813..4534
                     /locus_tag="PK733_00855"
     CDS             2813..4534
                     /locus_tag="PK733_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09128.1"
                     /translation="MLKRFISYYRPHIKLFTADLGCAFSMASIDLVFPAITRFLLQKV
                     IPDGNIKLLLIIIGVLVGLYAIRMMLNYFVNYYGHIVGIRMEYDMRKDLFSHLQTLSF
                     RFYDNVRTGKLMSRMMNDLNEITELAHHGPEDIFLSVIMLSGSIIILSTIEWRLALVV
                     FVIVSFMAWFAITKRTKMSRAFREVRSKVANVNAQLENSISGIRVSQAFTNEEYEKHK
                     FDDGNEQFKGSKDEAYNYMAQYSAGIDFITDMLNVSVIGFGGYLVYTKLIGITDLLTF
                     LLYINLAVQPVRRLAQFVQQFESGMTGFERFLEIMEEKPDIIESENAIELKDVKGRIE
                     FTDVTFSYNKGEDVLKGINLEIPEGKVVALVGPSGGGKTTLCNLIPRFYDVEEGSVKI
                     DDHDVRDISLKSLRGNIGVVQQDVFLFAGTIKDNILYGRVDATDEELIEAAKRANIHD
                     FVMSLPNGYDTYVGERGIQLSGGQKQRVSIARVFLKNPPILILDEATSSLDNETEMII
                     QESLEELSKGRTTLVIAHRLSTIRGADEIIVIDKDGVRESGTHSALIRLDGVYARLYN
                     TQFEGVV"
     gene            4697..6103
                     /gene="trkA"
                     /locus_tag="PK733_00860"
     CDS             4697..6103
                     /gene="trkA"
                     /locus_tag="PK733_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOJ09129.1"
                     /translation="MKVMIVGAGKLGYKLANAISGKDILITVMDSDPEVLGKISDHLD
                     ALMIKASGLQVDALKEAGIDAYDLIIAVTDSDETNIVVCSLAKKLGCKKAIARVRDPE
                     YASHREFIKSAMDIDYIINPELATAKEIIRYLLKSYSFYSGDFAEGRILMADFNALTL
                     PGFPGKQIKELENMSGLLIAAISRNGEIIIPHGSTRILDDDVIYIIGKRDNIDLIAKR
                     SKALLPVRNIKKVMILGGGKIGYYLGEKLADQGVAIKIIEQDKERCQYLSEHLNNVLV
                     ICGDGTDADLLMDEDISSMDAFIGVTGYDEENLLMTLMAKQAGVKKVIAKVSKSSYVH
                     LIEKLGVDIALNPIDITVGDILKFIRGGRAISVSLLLGGQAEVTEILADISMPVIGKP
                     IAELGLPKGIIIGAVSHKGKVFIPNGSSIINPGDRFVVFCLASQIHVLETFFKPSKGG
                     LFNELWNRGKSFGKSINH"
     gene            6054..7571
                     /locus_tag="PK733_00865"
     CDS             6054..7571
                     /locus_tag="PK733_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOJ09130.1"
                     /translation="MNFGIVVKVLGSLLIIEAAGMSLSLLVSIYFRQHDWLPFLISIL
                     LTALTGLAMLFFTTTTKRAIRKRTRDTIEGSVRNSTGKKETIKTKEGLTIVTLGWILV
                     SLFGSLPFIISGSIPSFVDAFFETVSGFTTTGATLIKDIEVLPYGILFWRSFTHWIGG
                     MGILVFTVAVLPALGVGSFQIFKAESPGPIADRIVPRIRDTAKILYITYIFMTFVEVI
                     LLVIGGMSVYESFVHTFGTVGTGGFSTRNLSIGAFNSTYINIVIAVFMVLSSTNFSLY
                     YMLYKGRWKEALKDQELRLFLGVIGVSVILIAINITGKFYTNIWKSLEHSFFQVSSII
                     STTGYATADFDQWPSFSKAILFMLMFIGGCAGSTGGGMTSARVLVMLKLIKREMQKVF
                     HPRAVIPLKLVEKTLSTDIIHSISSFFVLYIVIFVVGTIALSLEGISLTSASSAVAAT
                     LGNVGPGFEVVGPVRNYSQFSIPGKLLMSALMLVGRLELFTVIALFNKRLWRDEI"
     gene            7689..9842
                     /locus_tag="PK733_00870"
     CDS             7689..9842
                     /locus_tag="PK733_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOJ09131.1"
                     /translation="MADIITRLIQEFKLKPFQVQNTVKLIDDGCTIPFIARYRKEMTG
                     ELNDQILRELHERLVYLRNLEARKDGVKRLIEEAGKLTDEIADALQKAETLQEVEDIY
                     LPYKPKRRTRAAIAREKGLEPLAGIILLQAKFKGTLEDVVKPFINPEKGVENIDDALN
                     GAMDIIAEGISDNAEYRKAIREIFFKQGMIITKSKKDEDSVYRMYYDFREPVGKIAGH
                     RVLAINRGEKEGYLQVKIEVPDEQIIAGIKQKIVKNPPTATSVYVEKAAEDAYKRLIY
                     PSMENEIRNTITETAEEQAMKVFSENLKNLLMQPPIKGKVVLGLDPAYRTGCKLAVVD
                     ETGKVLDTTVIYPTPPQNKVEEAKKVVKYLIDKHKVDIISIGNGTASRESELFVAELL
                     KEIDRKVHYIIVNEAGASVYSASKIGAEEFPDFDVAQRSAASIARRLQDPLAELVKID
                     PKSIGVGQYQHDMNQKRLDESLKGVVEDSVNRVGVDLNTASPSLLSYVSGISAGVAKN
                     IVEYREEKGKFKGRQELTKVKRMGDKTFEQCAGFLRIPEGENVLDNTAVHPESYEAAL
                     KLLKAMGYGFEDVKNRKLGDMKKKIEQKGIENIAKTLDIGVPTLRDIVEELLKPGRDP
                     RDEMPKPVFHTDVMNIEDLKAGMLLTGTVRNVSDFGAFVDIGVHQDGLVHISQLSDRF
                     IRNPMEVVSVGDIVKVRVLDVDVERKRISLSMKGI"
     gene            9982..10902
                     /locus_tag="PK733_00875"
     CDS             9982..10902
                     /locus_tag="PK733_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09132.1"
                     /translation="MYKLLIVDDEYEIRNGLVKYFPWNKIGYQVVGQAENGKQAIDFI
                     KNNHVDIMLCDIKLPFMSGIDVARELYNMKSKVKIVFLSAHREFEYAKKALAYGVKDY
                     VVKSATYDELIDAFTFVKKELDNDRALKNATSGDATSGDAAREYRDTASGDGSCLAFQ
                     QASKNRPHLPSDANDEGIDEANINQEVFYAGINKKIINTVKDYVINNYKDANLEDAAA
                     LVYLSPHYLSKYFKQYTGVNFSDYLLMIRMNKAAELLKDIQYKTYEVSEMVGYSNSKN
                     FTRAFKNYFGKTPREFRQGENLEQGNMDLD"
     gene            10927..12849
                     /locus_tag="PK733_00880"
     CDS             10927..12849
                     /locus_tag="PK733_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ09133.1"
                     /translation="MRERIFLKNLIKYLIPMFVPVIVFGSLSIAITGKNIKEQINKNN
                     SNLLKQAKENIEIILNDADSINLNLMAYPDMISQVGEILNKPNMEADDYIAIGNTRKI
                     IDASAFSRPYVRSIYIYFDNMQKRLLTTSEGLVKIDNYYDKLWFDSYSKMDTSREFWV
                     EAREIRQYEFEKDRTDVITLYRKLYYTSVKRVGAIVLNFYTDYIDQLLSNLDMYPGQI
                     ILIMDENGNEIFSYCNEDETVGTGKTDIMGRTGIMSEAGKISKDQENEKYVENEEKQV
                     ETEVNLKELKENQSPFFVIEIDKDSYIINQLYSPKYNLKYISITPGKYLYSIPSQLTL
                     ITILLLVASLIIGTVLTLAFSRKNFNFINNIITVFEKAEKGEPLPSQPAKIKDEYSFI
                     IYNIIKVFLEHNYLKIQLSERKYKLKVMEMIALHSQINPHFLFNTLKTVYWKAIDLTG
                     GHNDVSEMVESLSTIIDYSLNSYEEFVTMSKEINNTKKYIDIQQIRYRDKFNVVWEYS
                     NEIENYKTIKLILQPLIENSIHHGINEKEGRCRIRIRITQVNNCLKITVIDNGNGINR
                     ENLIKIRKRLSQYESDKNSVRESGKNIGLYNTNRRLQLTYGEKYGLQINSKYGMGTVV
                     YVTIPLAVTTETQGRS"
     gene            13240..14601
                     /locus_tag="PK733_00885"
     CDS             13240..14601
                     /locus_tag="PK733_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ09134.1"
                     /translation="MKKVLRTTCVIIIVLSLLLSITACSKSGDGKKQTDNNVTQNQGT
                     EKSVEPPKPVTIRFAWWGGETRHKATVDALEKFHDKYPHITVQPEYSGWSGYFEKLLT
                     QFASKSAPDLIQISYANANEYVMRDQLYPLNDFINDGVLKVKDLPEVSLDMFKIDDKY
                     YAVPSGIMANAYLVYNKDIFDKYEVPYPREGFTWDDYFETARKLTKDTNGDGKTDIWG
                     TGNIFTDERVFFKMVYEQGGRLFSDDLKKVKFTGPEGVEVWTLAKNLLKEGIVVPPEI
                     EASNPPGVDSFRSEQCALEVIYAPAMYADIKFNWGIAKLPKTADTDIHWTVPSMIYVM
                     SNFSKAPKETAMLLDYILNDEEAGNILKMERGAPPNKNVRNNIASSLSETDKNVISII
                     DNVAQTSTFSIEPYPPGFIEVLNLLIRESQQVMYDKKTPEQAMKDLETEANKVIEKFF
                     NEK"
     gene            14744..15646
                     /locus_tag="PK733_00890"
     CDS             14744..15646
                     /locus_tag="PK733_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ09135.1"
                     /translation="MKKKFSGKNINSLERPFEGYLFIAPWLIGFLAFSLWPMINSAIL
                     AFADYNVFSGMRFIGLDNFRVMFTEDKMFGHSLLTTTIYVVVSVPVRLAAALLVALLL
                     NQKIRGMSIYRTIYYLPSVLATSVAMLVTWKMVLSREGIANKVLGFIGLGPYDFLSNP
                     DIALWTVSFLAIWQLGSAMVIFLAGLKAIPTELYEAAVVDGSSSWQRFFRITIPLLTP
                     TIFFNLVMGIIGAFQVFSVGFVMTRGGPANATYFYVLHLYRTAFESFRMGYASALAWV
                     LAIIVMALTLLVFRSSDKWVHYEN"
     gene            15762..16655
                     /locus_tag="PK733_00895"
     CDS             15762..16655
                     /locus_tag="PK733_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09136.1"
                     /translation="MSVNTVALKHNGKQRTKNGRERIAKLIIHLFLIIGSVIMTFPLI
                     WLISSAFKPSADIFDRNFRLIPETFTFKNFVNGWNINPQFSFGHFFMNTVVLTILVVI
                     GTAISSSLTAFGFARLKFKHKKLLFSIMLSTMMIPGQVTLIPQYLIFNRIGWLNSYLP
                     MVVPAFFGGGAFNIFLLIQFIRGIPKELDESARIEGCSSFRVYWNIVMPLCKPALFTV
                     VIFTFLGTWNDFMGQLIYLNSTEKFTVSLILNSLIDITSAVSWGAVIALTLLSVMPCI
                     LLFFVLQPYFVEGIATTGLKG"
     gene            16779..17138
                     /locus_tag="PK733_00900"
     CDS             16779..17138
                     /locus_tag="PK733_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09137.1"
                     /translation="MQLLVLVLNKVELLDELLKSFVSAGIKGATILDSMGMARALTSG
                     GNEDIPIFGSLRLILNEGHPYNKTLFVVLKDDEVNICVRCIREVVGDLNKPDVGILFT
                     VPVNFIEGLTKKNQEGK"
     gene            17151..18350
                     /locus_tag="PK733_00905"
     CDS             17151..18350
                     /locus_tag="PK733_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ09138.1"
                     /translation="MNTLFYVSIVLLAGLLTAKLTSLAKLPNVTGYLVGGLIIGPSIL
                     RVVPKDAVTHMGIISEAALGMIAFSIGSEFSFKHLKQLGSGIITLTVIQALSAFAAIV
                     TAIVLIFGKSIEVGLVLGAIGVATAPAATLLVVRQYKASGPVVDTLLPVVAIDDAVGI
                     MVFGLCMAVAKAVHMPEQNVSLAMSIIKPIWEIGLAVIIGFLLGLLLSFIATKVRGDE
                     ELLGISLAMVFMGVALAGKFGVSSLLLCMTMGGVLTNLASNSQKVFSMVDRFTPPIYV
                     AFFTLSGADLDLSILKSVGLLGIGYIISRSIGKIVGAYVGAKIIKAPPTVQKYLGITL
                     LPQAGVAIGLSLVAQSVLPEFGASIRTVVLFATLIYELVGPVLTKIALTGAGEIQPVK
                     SKAQLTR"
     gene            complement(18499..19209)
                     /locus_tag="PK733_00910"
     CDS             complement(18499..19209)
                     /locus_tag="PK733_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09139.1"
                     /translation="MLQLNLEKVIGPIRNNVALIKKILIIAFSVLLIFTSIFGVYNYV
                     AMNKAISGLKGEIETLTADLNKYKSKDNNNTSEIPELKDYNEFKIKTMTDLALTKLML
                     DQFNKAVFNVSESGKGFARIDTSTGMFFLILDKAELQGEGYKLYFRLGNPQNCVYNGC
                     KLKVRWGVKYDREDKSINHDDWDKSLSSADFSVSNVLRPGEWTNFDIVISPAKAESAQ
                     YLEIKLQTDQIIMQKSTK"
     gene            19581..20339
                     /locus_tag="PK733_00915"
     CDS             19581..20339
                     /locus_tag="PK733_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HOJ09140.1"
                     /translation="MKGNTILINILMMAVIFVLAFSFVAVLRHTLADNNIFAEVASET
                     SLKITEKQIKETKGNLDMEFSIPVIEGLADKEIQSSINSMLENDIESFRKYMIELSAT
                     DTDSSINMKYIAKTSYKINKKSRNLLSISVIYYQDTGGAHGIYDMVAYNFDIRNGSLL
                     ELDDLFENKDGYKDIINEEIRRQICENKGKIEYFSGDSGFKSISSHQPFYINDGKLVI
                     YFSLYEIAPYSSGIPGFEIPFTLFEENLNEIIAQ"
     gene            20469..20726
                     /locus_tag="PK733_00920"
     CDS             20469..20726
                     /locus_tag="PK733_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09141.1"
                     /translation="MSMFEMLMLLCFGAAWPVSIYKSYTSRKNSGKSLGFLIIILIGY
                     ISGVIHKILYSRDPVIVLYILNSIMVSIDIALYFRNKKASN"
     gene            complement(20797..21285)
                     /locus_tag="PK733_00925"
     CDS             complement(20797..21285)
                     /locus_tag="PK733_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOJ09142.1"
                     /translation="MYKFVNKKNKSLRFITEAAVIGAIYAVLTIVFSPISYGLFQVRI
                     AEALTVLPAFTPAAIPGLFIGCIISNIIGGNGPLDIIFGSLATLIAAILSYKMPKRYL
                     VPLPPVLVNAVVVGIMLSYILKVSVLVAMGWVALGQAVACYGVGYILMMQLEKYRSKL
                     FK"
     gene            21643..22731
                     /locus_tag="PK733_00930"
     CDS             21643..22731
                     /locus_tag="PK733_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ09143.1"
                     /translation="MIENSKVAVLETTPETVIEDYKKLMRMSDYDKYLKKDRPTILKN
                     NISWHFPYPGANTTPWQMEAVIQCLREDGYKDIVCVENKTVVTNAYKGERLNKYVDIF
                     KKYNIPVLYNFKEEDMKWVKYEPKGEILVLDKIFPDGIYIPDYFFDKNIIHLPTMKCH
                     IYATTTGAMKNAFGGLLNTKRHYCHSEIHKTLVDLLTIQKEIHSGIFAVVDGTVSGNG
                     PGPRTMKPAVTNYILAGGDQVAVDAVSSKIMGFDPMSLEYIREGNNAGLGNGRMENIE
                     VVGEDISNVNWGYTVGDNTASKVGDLLWFGPLKKLQRLMFHTPIVNIFIWGSAFYHDV
                     IWYNLQGKKVVKDYQNNTQWGRLFKSYK"
     gene            complement(22925..24112)
                     /locus_tag="PK733_00935"
     CDS             complement(22925..24112)
                     /locus_tag="PK733_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOJ09144.1"
                     /translation="MDKNRKNELYKFYKSSLLDDCIPFWLNHSLDHKYGGYLTSVDRT
                     GKVFNTDKSVWFQGRGLWIYSRLCNTLGKKDEWLEAAHLGYNFIMKHCFDDDGRMFFQ
                     VTQDGKPLRKRRYLFSETFAIIALAEYYKTTGNTAALDKAKSIYKMVLSIYENSANDP
                     YKITPKVYAETRASKAMAVPMILLNTNYILRETDMGNAGNAYDHIAKRLVDEILKDFL
                     KPEVKALFETVGLNGERLDTPAGRCINPGHSIEASWFLMKEAKYQKDDALMQKALDIL
                     NWSLDLGWDKEYGGILYFVDIDGRPEEQLEWDMKLWWPHTEALYALLLAHKYTGKQEY
                     MDWYEKVHSWAFSRFPDKEYGEWYGYLHRDGTVSHTLKGSMWKGPFHLPRALLFCMQL
                     LEE"
     gene            complement(24267..25730)
                     /locus_tag="PK733_00940"
     CDS             complement(24267..25730)
                     /locus_tag="PK733_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ09145.1"
                     /translation="MIYSRKKYEPIQKIIRAMECTNNDQNYNVNKKFKGNWQRDIPLI
                     FDRLEKGREKLVSIFEDIFKAAFLTSSFDLKMKFFGEKVNNIGSKINNMSFDVHKSSE
                     SSLASATEITNANTEIAYSIENITLRANQLKENTTDNRNVVNQISIDTKNALVYSQSM
                     KDDFKILQESLDSMKEIVDGIYEISDQTNLLAFNASIEAARAGEAGRGFSVVAEEIRK
                     LSDSTKNLLTSIGNIVNDIYNNSAKSSESIEHTADSLTNINTSIAKMMSSIESSNQSI
                     TGIASSLENISAVSEEITSSLEELTNFMNSLTLMSGELRIHSSSLESTGKDMENLSEA
                     MLILENNLDDATKKCGEIILDRLYSIPNEKFSEFIEEAIKNHKRWVSELESMVEAMEI
                     RPLQTNDHNCSFGHFYYSVKPSHPEILNSWNKIEDIHHGFHNTGNVVINSINAGNREA
                     TIKATEEAKKLSESMIDILSELIEKSKELTEKNEFVF"
     gene            25976..26428
                     /gene="mscL"
                     /locus_tag="PK733_00945"
     CDS             25976..26428
                     /gene="mscL"
                     /locus_tag="PK733_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOJ09146.1"
                     /translation="MWKEFKQFAVKGNIIDMAMGIIIGSAFSKIVSSLVNDMIMPIFS
                     LIIGRIDFTNLFIALDGKHYEKLGDAKEAAVSTINYGMFISAIIDFLIIAFSLFLIIR
                     NVNKLKKKEEAPPAEPTAKVCPYCQSTISIKATRCPQCTSELVETMKV"
     gene            26897..27976
                     /locus_tag="PK733_00950"
     CDS             26897..27976
                     /locus_tag="PK733_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ09147.1"
                     /translation="MDFNSNTKIIDSHVHIRSLKSMEFLYNYLKNYNLKKINIVSLAC
                     KDQSEIAQNLLCALFKLLHPKSVYAYGSLMYPYYPLDEKIPYNYNLVEQVKTLMNMGF
                     DGIKMLEGKPTLRKKIGIPLDSPIFDDYYSYLEEQEIPIVFHTADPETFWDRETAPEF
                     SFEQGWFYGDGTFPGKETIYNEIDGILSKFPKLRVTFAHFYFLSNYIERAEAFLNKWK
                     NVSLDITPGREMYDNFSRKPSEWKAFFTKYQDRIVFGTDMEDSMFQGGPSDIINTIRR
                     FLETGDKFHNWGFEINGLALDKNVIDKIYYKNFESYAGSNPKMVNTKRLLDECRRIKG
                     IALESPALSTVVPEINKIIENITSR"
     gene            28134..28826
                     /locus_tag="PK733_00955"
     CDS             28134..28826
                     /locus_tag="PK733_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09148.1"
                     /translation="MDKNDKKSNTLKFKKYLNSLSIKFIVCTAAVTLISVLSIGLFSF
                     LYISNSINEEITRYNTQRLNYVKETVDNRVFGKTLNILSGLLVKNEFNELLYYYLQHP
                     KTNNLTKVNSVMTILKDKALTNSDILSSVSIYYKNNDIVISSSSGIKYLNDTNKNLPF
                     DDEWIDIFDDMRKSNKGRNQNVIWIPTRETPCSYGYTANEYNISLIGKFPQNYVQEGY
                     DSLVCLNISEKV"
     gene            28799..30529
                     /locus_tag="PK733_00960"
     CDS             28799..30529
                     /locus_tag="PK733_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ09149.1"
                     /translation="MFEYQRKSIRNTINDIVKDDEINVLIVDSKGNVMSDNNGKSICS
                     DISNSGIFNVVNQDSGEHDIETKSLIEIDGIETVISSVKSDYCGWYYILMAPSSVYFQ
                     KTVIARKIIFTLCVIIFFVLLFVVMLISVKLTYPFNRILTNAKNVLGEKRSEVYRNEL
                     LCINDALNMLSQKTNVQEQILNGNRDLVRQTFFLNLMSGQKFNDNDIEKTLEFLDQDM
                     SLPYFTVAIIQNNMFAKADSLFNKIENDIYHYIDNREYDYINRDLNIIYIKYNEHHIS
                     LIINHLIPSPKIAADFVNNLKEYIEKRFDAILHYSIGTVCNKINQIDRSFYDAYDALA
                     YSSIYPTIRIFEHDITKKWDKNNNLEYGYIDSFVKSLNEYRLDNCKQYLYIIMNNVIE
                     EHLSYKTYKSIINEIILSIEKLVINTKIDLSEIFSVSIAEQVESQYYIIDVFKTIECI
                     LGKILDHIVSQHSSLNSVYVAEAKKFIDEYYYKDISIQDIADKLNISRHHLCRVFKET
                     TANTLLNYITEVRMNKARELLAEGKLPINDIAKEIGFNNTTYFNKKFKQNFGVTPTQY
                     QSNVSCEKTV"
     gene            30714..31712
                     /locus_tag="PK733_00965"
     CDS             30714..31712
                     /locus_tag="PK733_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09150.1"
                     /translation="MNPSKVTASASDIKYGKKLSGKNSKSCKNSKVSKYFSECADNYQ
                     LVLMFLPVIICLALFSYAPMYGLVIAFKDFQLLKGISGSEWVGLENFRKLLITPSFHE
                     VFRNTVVISLLRILFGFPAPIILSILLNEIYHNTYKKIVQTISYLPHFLSWVVLGGLF
                     MQVLSPSGGLVNQVITAFGGKPIYFLADPNYFIGMLIATGIWQSAGWGTIIYLAAITS
                     INTELYEAAVVDGATRFKQIIHITLPSIAPTITILFILQMGNIVNAGFDQIYNLYNPA
                     VYKVADIIDTYMYRKGLISMQYSFASAAGLFKNIISFILVFLTNCVVKKFNEYSIW"
     gene            31783..32670
                     /locus_tag="PK733_00970"
     CDS             31783..32670
                     /locus_tag="PK733_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09151.1"
                     /translation="MSRILKKTLGDKIFDVVNYIFMFIFCLTILLPFIHLLAQSFDKV
                     GNISAEGLYIWPEEFTFDNYKMIAKNRYIWNGYYNTFFRTIVGTGMSLFFTSMGAYAL
                     SKKRFPNRTFWTMVVVFTMFFSGGLIPTFLWIKKLGLLDKRMVLILPGLIGAYNLVLI
                     RNFFQQIPGELEESARIDGARDFTVFLRIILPVSKPILATVALWLAVGHWNAWFDCLV
                     YIKDTKKYVVQVILQRIILQGTQQMMMDDTMVQKEYESRPDVIKAACIYFTTIPILCV
                     YPFLQKYFVKGVFIGSLKG"
     gene            32867..36916
                     /locus_tag="PK733_00975"
     CDS             32867..36916
                     /locus_tag="PK733_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09152.1"
                     /translation="MKTAKKITALILSVIFTVALLAGCGGKKNGNAAEPTKKGTTGTT
                     EKAGSNTDNKQEETYTYTIIQWLPAPLDDNAPMVKKWEDEFGVKFKFDYIETTKITEL
                     LPLRIASGDIPDIITSLDYNGFYDYVKQGIAGTWDEEFFRKNASYISKLMDEMGEDSW
                     KISKYNEKMFTVPGINCEYMYGAAMIWRNDWLKNLGITEIPYKLDQVEAALYKFRNGD
                     PDGNKQKDTYGLSQDGMKMILGAFGAPEKNWIDDGNGGVIYSSIAPQTKDALVLLRKW
                     YKDEVLDPEFITGENKGGYWALSHAFCNGRIGFTARAVNAHWKDPKLWELGEAPYLKE
                     VKATRLPMPEGKTMQISEDKTDLNDPTIYLTALGTDIKGYIAYLTGDTQMLEDYFTVW
                     QKLLEMEVNHLVLYKKAVVWRILEMTGMLPEELRQPLLNFFYKWANSEEGIGSIDHSL
                     YQSPYYPRQNHGLIPALGLVYLSDYFTKFHADLKEPYIWKEKADKVYAPYFSGSWKPV
                     CDGLCHGWWLSQPAMLEYGLMDDKHRYFEGGGARKAAECAMTVVNSEGWMPCSGDSDI
                     LRQFPGFSLRIAAAYYNDGRYRFVNEMAPFAQRGYCGPITYPPRAFDTGVQKEEPCDK
                     IGIDVIPIDPLVYYAWEKEPKLASGASDAPPKAPIDKCFDKLAIRTGWGRDDDYLLID
                     GLGGGSHSYADAMSILDYQRFGVSFIVAEDSLHWPEPENHSMVTIYKDGKKEKIPSFA
                     ELMETVTDGKGNMYAKMKLKDYNGADWIREIYMIPEKCVVFHDTIIANDFGNYTIENH
                     FRTPGIAEFENTAGNIIRSKRKTRNGETVEFKLLSKCSRNSTYSMEKIPIDLKYRVLP
                     GSELPFSEETNDAAAGRKRYHLRDICLSVFTARTAVKLNKGEQVKFTHVACADKNSVN
                     IEILENSIKMLENNSKILENEHSYKESRKPVNINLKIGDEIYTLPFSLESINAGNVDN
                     VGDVGDVGNVGSTTNISISEIKRGYEIKTVCEESSAITAAQLVQDKYLLCGLKDGTVT
                     MYDVEGNTIWSSEVDGKIHDVSLLEDKGTKTVFAAHGKAGITAVNEKGEVLWSKKITR
                     IPTLYPWWEFDDPTPVKIKTGILNGEGLALIGCGDNYVRFYDKDGNMKDAYYYFASVP
                     AYIHIFDVDSDGEQEVIVGGGIISADSGIDILDSKGKALTRFGSEGWVSKTTAFEFAQ
                     MNGYNVIACGVNHRTNLHLYRVESSNPDQNKISYRRIVYNELAGAVTGIALIPYYERI
                     FAGTSQGFVSAYDFHGKELWMEMMEGAVTDIAAWKNSIIVTEKTGKVNIMTLDGKLEA
                     STKLDTAAANILKGSKNLYFISGNCILKI"
     gene            complement(37103..37303)
                     /locus_tag="PK733_00980"
     CDS             complement(37103..37303)
                     /locus_tag="PK733_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09153.1"
                     /translation="MEDSINLKEICNWAELFDMTFEYLTFLLEVEDIKPDLVMKTTLD
                     ILNNAGYSFSFDEVEEEYYKCL"
     gene            37449..37757
                     /locus_tag="PK733_00985"
     CDS             37449..37757
                     /locus_tag="PK733_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOJ09154.1"
                     /translation="MKVIKKDGRVQPFDLSKIIFALERISDECSQPFTQSDLTFVAKN
                     IEKSIRAAMENKDEDIIHTSKIRTIVIKELEQAGFKHIADCYKEYVKGFGKPDSPNNI
                     "
     gene            complement(37877..38035)
                     /locus_tag="PK733_00990"
     CDS             complement(37877..38035)
                     /locus_tag="PK733_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOJ09155.1"
                     /translation="MDKYVCTACGYVYDPAEGDPDGGIAPGTAFEDIPEDWVCPICGV
                     GKDMFEKE"
     gene            complement(38199..38783)
                     /gene="yyaC"
                     /locus_tag="PK733_00995"
     CDS             complement(38199..38783)
                     /gene="yyaC"
                     /locus_tag="PK733_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOJ09156.1"
                     /translation="MFIKTSVEKHFIDINSPGAFHYFADVLYKLICKDISDGYKSIVF
                     VCIGTDRSTGDSLGPLIGHKIMNMNNRKNISVYGTLENPVHAKNLSKVMEDININCIN
                     PFIIAIDACLGKMEHVGFVSIGEGPINPGSALKKELPPIGNIHIVGIVNLGGFMDFLI
                     LQNTRLFLVMKMADLIAGGIKHVLWRIQKDPFLF"
     gene            39091..40269
                     /locus_tag="PK733_01000"
     CDS             39091..40269
                     /locus_tag="PK733_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ09157.1"
                     /translation="MYPELAYDTNDTREKNSETSIYNTDGYLVEGEMLPIEDVINGML
                     DWVRVLDLNGNIIYINKAMEEALKRPVVGKRCYEAFGRAEPCKDCVSHKCAFDGKTHE
                     KEEVFDDRYFSVMSSPIKNKHGDVVAIVEVLRDVTKVKLLQKQIMEQNRKHRDDIEMA
                     KKIQLSLLPVKLPKDKAEFSYIYTPCETLGGDFLDIFMIDDDHFGLYIADVAGHGITS
                     SLLTVFLKSTFNKETLSPSLALMELYREYNKMGFDTDLYITVFYSIIDLKNNTITYSN
                     AGHNICPILFSSTRFEILRNAGLPICDWSEKPDYIDKSELLFKKDRVFFCTDGIIEVR
                     NNLNEQFGEERLLYNLAACKFCPDVVLKSIIEQACKFAGVDEISTIGDDITMALLEIV
                     "
     gene            40283..41617
                     /gene="glnA"
                     /locus_tag="PK733_01005"
     CDS             40283..41617
                     /gene="glnA"
                     /locus_tag="PK733_01005"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOJ09158.1"
                     /translation="MQKYTEEDILRMVKEQDVKFIRLQFTDIFGILKNVAIPASQLEK
                     ALNNKCMFDGSSIEGFVRIEESDMYLWPDLNTFVIFPWKPQTGKVARLICDVHNPDGM
                     PFEGDPRYVLKKVLKKAEDMGFDTLNVGPECEFFLFLTGSEGNPTVKTQDNAGYFDLG
                     PVDLGESARRDIVVALEEMGFDVEASHHEGAPGQHEIDFKYADALTAADSILTFKLVV
                     KTIAQKHGLHASFMPKPIYGINGSGMHTNISLFTSGVNAFYDEKDQVKLSKEAYWFIG
                     GLMKHARAIAVLTNPIVNSYKRLVPGYEAPVYIAWSARNRSPLIRIPAARGANTRVEL
                     RCPDPSCNPYLALAGILSAGLDGIENRICPSNPTDKNIFCMSDEQMQKEGIFSLPGNL
                     KEAVDEMAKSSLLKKVLGNHIFEKYIEAKNEEWEDYRKQVTPWELKHYLTKY"
     gene            41670..>41848
                     /locus_tag="PK733_01010"
     CDS             41670..>41848
                     /locus_tag="PK733_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOJ09159.1"
                     /translation="MPAKRPLIGITPGYSYEEQKIYINNGYIDAVNQAGGLAILLPLT
                     DDEELVSVLVDTCDA"
BASE COUNT        14236 a         6392 c         8310 g        12910 t
ORIGIN      
        1 actttgttca aattttatac tcggaaaatc gtttgctgcc atattttgta tcataagaag
       61 aaaatgcacc cgtatgagaa caggatactt tttgccttta ttcctaattt atggtataat
      121 gcttgtgagg tggtgctgat attatggagt atctgagtgg gatagtggag aggatcactt
      181 tttccaatga agaaaacggt tttagtgtta taaagataaa atctaaaggt tttgccgacc
      241 ttgttactgt tgtgggaagc ctggccgctg tcaatgttgg agcgactgta aagctgaaag
      301 gcgagtggaa atatgatagt aaatatggga aacagtttag tgcgatagaa tatcaagaga
      361 ctgtaccggc aactatagcc ggaattgaaa agtatctggg aagcggcctt attaaaggag
      421 taggaccggt ttatgctaga aaaatagtga ggaagtttaa agaagatact ataagaatta
      481 ttgatgaaga atctgacagg ctaaccgagg tagatgggat tggaactaaa aggctggcaa
      541 tgattaaaaa ggcatggcag gagcaaaaag aaataaaaaa tgtcatgctg tttttgcaga
      601 gcaacggcgt ttctactgca tatgctgtaa agatttataa aacttatgga aatgaaagta
      661 tagaaattgt aaaaaacaat ccttataggc tggctgatga tatatggggc ataggtttta
      721 aaactgctga taaaattgcc cggcagcttg gttttgataa aaattcctat gaacgctgcc
      781 gttcaggtat aatatatgta cttaatgagt tatcaaatga cggacattgt tatgctaaaa
      841 gagagcagct tattgatgaa gcagaaaaaa tgcttgaatt ggaaaaagag cttatagatt
      901 caaccataga taaaatgatt tcagagaaaa caataataat ggatgctgaa gattgtatat
      961 atttaccgcc tttctatttt agtgagacag gaactgcaaa gcgaattaaa gaaatattat
     1021 ctgttgagag cgtctacaag aattttgata cggataaaat tataaaacag gttcaggaag
     1081 aatgtgatat agagtatgac aaagttcagg ttgaagccat aaagaccgct gcgaaatcaa
     1141 aattcatggt gcttaccgga ggacccggaa ccggcaaaac caccacaact ttagctataa
     1201 tcaaggtttt ccaaaaaatg aatgcaaagg ttctgcttgc agcacctaca ggaagagctg
     1261 caaaaagact atccgaaacc acaagcatgg aggcaaagac tatacatagg ttattagagt
     1321 ataaacctcc cgaaggatat aaaaaaaatg aagaaagtcc gctggattgt gatgtactta
     1381 taatcgatga aacttccatg gtggatatta ttctaatgta caatttacta aaagcagtac
     1441 ctgtacaagc tgttttgata cttgtaggtg atgttgacca actgccatcg gtaggtgccg
     1501 gaaatgcttt aaaggatata ataaattccg gtgtcgtaga tgtggtttgt cttaccagaa
     1561 ttttcagaca agcccttgga agtgccataa taacaaatgc acatagaata aataagggcg
     1621 aatttccagt attaagaggg gggcgggata atgacttttt ctttatagag gaagaagatc
     1681 cccaaaaaat agtagaaata ataaaagagc tttgtgcaaa aagattgccc gggtattaca
     1741 aagcagatcc tataaatgac attcaggtgc ttagccccat gcaaaggggt gaaacgggag
     1801 ctttcaatct taaccaggtg ctccaggaaa ccctgaatcc atcagaaatt tgcataaaat
     1861 atggggggac aacatatcgt cttaatgata aagtcatgca aataaaaaac aattatgata
     1921 aaaatgtgtt taatggagat attggcttaa tcacttcaat tgatatggag gataaaactc
     1981 ttgtaatcac atttgatggc aatgatgtgg aatatgatgt gacggagctt gatgaagttt
     2041 tattagctta tgctacaact gtacataaga gtcagggcag cgaatataaa attgttgttg
     2101 cacctttaac tacacagcat tacatgatgc tgcaaaggaa tttggtttat acctgtgtaa
     2161 ccagggctaa aaaggtcctt gtgctggtag gtacaaaaaa agcgattggt atagcagtgt
     2221 caaatgataa aacccaaaaa agaaacacta tgctggaagt aagattaaaa aactaggaaa
     2281 aacaagagca tagaaaagtt atagaaggat gaaataagga tgataggagt aggtaaacat
     2341 tgttacaaat tgatcaaaca aatactggat gatataatat cctcataatt cttgtaaacg
     2401 aggatattat atgcctccgg tggaatttat gtgcgtgcga atatttttgc tttgcaaaaa
     2461 acgcaccatg cacaattata tcatgcaatt tcttcgaaat tcatgatata atatcctcac
     2521 aaatgaggat attatatgcc tccggcggaa tttatgtgcg tgcgaatatt tttgcttcgc
     2581 aaaaaacgca catgcacaat tatatcatgc aatttcttcg aaattcatga tataatattg
     2641 ctagtcaaag tatgaggaga caattatacg aggaggtcta atatgccaga aaaaaatatt
     2701 ggaaagatcc tagaggaagt tactaaaaat gcaaaaaaat gagggagttt ttatatatga
     2761 acaataaaat aataacgcta gggagattat tttaatgtta tctaaaaaaa atatattgaa
     2821 gagatttatt tcatattata ggccccatat aaaattgttt actgccgacc tgggctgtgc
     2881 cttttcaatg gcatcaatag atttggtgtt tccggcaatt acccgctttt tgcttcaaaa
     2941 ggtaattcct gatggtaata ttaaattatt gttaataatt attggggttt tggttggatt
     3001 gtatgcaata cgtatgatgt tgaattattt cgtcaattat tatggacata ttgtaggaat
     3061 aagaatggag tatgacatga gaaaagacct tttctcccac ctccaaacct tatccttcag
     3121 gttctatgat aatgtcagga ctgggaaact tatgtccagg atgatgaatg atttaaatga
     3181 aataacagag cttgcccatc atggaccaga ggatattttt ctatcagtta taatgctttc
     3241 cggttccatt attattctgt caactattga atggagactt gcccttgtgg tgtttgttat
     3301 agtttctttt atggcctggt ttgcaataac aaagaggaca aaaatgtcca gagctttccg
     3361 cgaggtcagg tcaaaggttg ctaatgttaa tgcccagctt gagaatagta tttcgggtat
     3421 ccgtgtttct caggctttta caaatgaaga gtatgagaaa cataaatttg atgatgggaa
     3481 tgagcaattc aagggctcaa aagatgaagc atataattat atggcccaat attcagctgg
     3541 gatagacttt attacagata tgctaaatgt atcagttatt gggtttggtg ggtatcttgt
     3601 ttatacaaag ttgataggta ttaccgatct attgactttc ctgctttata taaatctggc
     3661 tgtgcagcct gtcagaaggc ttgcacaatt tgtgcagcag tttgaatcag ggatgacagg
     3721 ttttgagcgt ttcttagaga ttatggaaga aaaacctgat ataattgaaa gtgaaaatgc
     3781 tatagaacta aaagatgtaa aagggcgtat agagtttact gatgttacat tttcatataa
     3841 taaaggcgaa gatgtgctta aaggcattaa cctggaaatt cctgaaggca aggttgttgc
     3901 cctggtcgga ccttcggggg gagggaaaac gacactatgc aacctaatac ccaggtttta
     3961 tgacgtggaa gaaggttctg ttaagataga cgatcatgat gtaagggata tatctctgaa
     4021 gtcattaaga ggaaatatag gtgttgtaca gcaggatgta tttctttttg cagggaccat
     4081 taaagataac atactttacg gcagagtaga tgcaacagat gaagaactta tagaagctgc
     4141 aaagcgtgca aatatacatg actttgttat gagcctgcct aatggctatg atacttatgt
     4201 aggtgagaga ggaatacagc tttccggagg acaaaaacag agagtatcca ttgcacgtgt
     4261 atttttaaag aatcctccca tattgatact tgatgaagca acttcatctc ttgataatga
     4321 aacggaaatg ataattcagg aatcattgga agaattatcc aaggggagaa ctactcttgt
     4381 tatagctcac aggttatcta caataagagg ggctgatgaa attatagtta ttgataaaga
     4441 cggggttagg gaatcaggta ctcactcggc tcttatacgg ctggatggag tatatgccag
     4501 gttatataat actcaatttg aaggtgttgt ataaggcaaa gagtcatagt ataagaaatc
     4561 aaaggatgta gaatagcaaa gacaataggg aaaattagag aaaagtagag aatggattga
     4621 atatgaaaaa aatgctcatg tacagtggaa aaatagtttg ctgatgcacc cgagcggaat
     4681 ggtggttaat attcttatga aagttatgat agttggagct gggaagcttg gttataagct
     4741 tgccaatgca atatccggga aagatatact tattactgta atggattccg atcctgaagt
     4801 tctggggaaa ataagtgacc atcttgatgc cctgatgatc aaggcaagtg gtcttcaggt
     4861 ggatgccctc aaggaagcag gaattgatgc ctatgatctt attattgccg ttactgacag
     4921 cgacgagacc aatattgttg tatgcagtct tgctaaaaag cttggctgca agaaagcaat
     4981 agcacgtgtg agagatcctg aatatgcaag ccacagggaa tttataaagt ctgctatgga
     5041 tattgattac ataataaatc cggagcttgc tacagcaaag gagataataa gatatcttct
     5101 gaaaagctat tcgttttatt ctggtgattt tgctgaagga agaattctaa tggctgattt
     5161 taatgcattg actcttccag gtttccccgg gaaacagatt aaagaacttg agaatatgag
     5221 tggccttcta attgctgcaa tatcaaggaa tggagagata attatacccc atggttcaac
     5281 caggatactg gatgatgatg taatatacat aattggaaaa agggataata ttgatcttat
     5341 agctaaaaga agcaaggctc tcctccctgt taggaatata aagaaagtaa tgattttggg
     5401 tggagggaaa ataggctatt atcttggaga aaaacttgca gaccagggtg tagctataaa
     5461 gattattgag caagataaag agcgatgtca atatctttcc gagcatttaa ataatgtact
     5521 tgttatttgt ggtgatggta ccgatgctga cctgctgatg gatgaggata tttcctcaat
     5581 ggatgctttt ataggagtta cgggatatga tgaagaaaat cttcttatga ctctgatggc
     5641 caagcaggcc ggagtaaaga aggttattgc aaaggtaagc aaatcaagct atgtgcatct
     5701 gattgaaaaa ttaggagttg atattgcttt aaatcctatt gatattacgg taggagatat
     5761 tttaaagttt atcagaggcg gcagagctat atctgtatcc ctcctccttg gaggtcaggc
     5821 tgaagttacc gaaatactcg cagacatcag tatgcccgtt ataggaaaac caatagcaga
     5881 gctgggactg ccaaagggta ttattatagg agctgtctct cataagggga aagtgtttat
     5941 tcccaatgga agctctataa ttaatccggg agacagattc gttgttttct gtctggcttc
     6001 acaaatacat gtgcttgaaa ctttctttaa gccttctaag gggggactgt ttaatgaact
     6061 ttggaatcgt ggtaaaagtt ttgggaagtc tattaatcat tgaagcggca ggaatgtccc
     6121 tttcattatt agtatcaata tatttcagac agcatgattg gttgcctttt cttataagta
     6181 ttttattaac ggcgttaaca ggattagcaa tgttattttt taccacaacg acaaagaggg
     6241 caataagaaa aagaacaaga gatacaatag aaggatcagt tagaaactca acagggaaga
     6301 aggaaactat taaaacaaaa gaaggtctga ctattgttac cctgggctgg atacttgtgt
     6361 ctctttttgg gtctttgcct tttattattt ccggcagtat tccatctttt gtagatgcgt
     6421 ttttcgaaac cgtttcaggc tttacaacta caggagctac acttataaaa gatattgaag
     6481 tgctgcccta tggaatactt ttttggcggt catttactca ctggataggt ggtatgggca
     6541 tacttgtttt tacagttgca gttctgcctg ctttgggtgt gggaagtttt cagattttta
     6601 aagctgaaag cccgggacct attgcagata gaatcgtacc tagaattaga gatactgcta
     6661 aaatattata tattacatat atttttatga catttgttga agttatactt ttagtaattg
     6721 gcggaatgtc agtatacgaa tcctttgttc acacatttgg tacagttggt acaggaggat
     6781 tttctaccag gaacttaagt atcggagctt ttaacagcac atatattaat attgttattg
     6841 cagtattcat ggtattatct tcaactaatt tctcccttta ttatatgttg tataaaggca
     6901 gatggaaaga agctttaaaa gatcaggaat tgcggttatt tcttggcgtt ataggggtta
     6961 gtgtgattct aatagccatc aacattaccg gcaaattcta tacaaatata tggaaatctt
     7021 tagaacattc tttttttcag gtgagttcaa ttatatctac aacgggatat gcaactgctg
     7081 attttgacca atggccgtca ttcagcaagg cgattctttt catgcttatg tttataggag
     7141 gatgtgcggg ttctaccgga gggggtatga caagtgccag ggttcttgta atgcttaagc
     7201 tgatcaagcg ggaaatgcaa aaagtatttc atcccagagc ggtaattccc cttaaactgg
     7261 ttgaaaaaac actttcaaca gatattatcc acagtatatc cagttttttt gttctttaca
     7321 tagtcatttt tgttgttgga acaatagctc tttcactaga agggataagt cttaccagtg
     7381 catctagtgc tgtagctgca actcttggca atgtaggacc cgggtttgaa gtagtaggac
     7441 ctgtacgaaa ctacagccag ttcagcatac ccggcaagtt actaatgtca gcccttatgc
     7501 tggtagggag gctggagttg tttacggtaa ttgctttgtt taacaaaagg ctctggcgtg
     7561 atgagatttg ataaatgcac cctgccttca ttgactcaat gacaaacgta aatttttatg
     7621 ataaaatctt tgtacgaaac aattgaagaa catcaatcgt acagacaatg actacaggga
     7681 ggattatcat ggcggatatt attaccagac ttattcagga gtttaaacta aagccttttc
     7741 aggtacagaa tacagtaaaa ctaatagatg atggctgtac catacctttt attgcccggt
     7801 acaggaaaga aatgacagga gagcttaatg atcagattct cagggagtta catgaaagat
     7861 tagtttattt gagaaatctt gaggccagga aggatggtgt aaaaaggctt atagaagagg
     7921 cagggaagct tactgatgaa attgctgatg ctctgcaaaa agctgaaaca cttcaggaag
     7981 tagaagatat atatcttcca tataaaccca aacgtcgtac aagagcggct attgcaagag
     8041 aaaaggggct cgagcctctg gcaggtatta tattattgca ggcgaaattt aaaggaacac
     8101 tggaagacgt agttaaaccc tttataaatc cggaaaaagg tgtggaaaac atagatgatg
     8161 ctctaaatgg ggcaatggat attattgctg aaggaatatc cgacaatgcg gaatacagga
     8221 aagctataag ggaaatattc ttcaaacagg gaatgattat aaccaaatcc aaaaaggatg
     8281 aggattcagt atacaggatg tactatgatt tcagagaacc tgttggcaaa attgccggac
     8341 acagggttct ggcaattaac aggggagaaa aggaaggata tttgcaggtt aaaattgaag
     8401 tgcctgatga gcagataatt gcaggtataa aacaaaaaat agtcaagaat cctcccacag
     8461 caacatccgt atatgttgaa aaagcagcag aggatgcata taagcgatta atatatcctt
     8521 caatggaaaa cgaaatacgt aacacaataa ctgaaacagc agaagaacag gcaatgaaag
     8581 tattcagtga gaatctgaaa aatctgctga tgcagcctcc tataaaaggc aaagtagtat
     8641 tgggattgga tcctgcatat aggacaggat gtaaacttgc tgtagttgat gaaacaggga
     8701 aagtacttga tactacagta atttatccta ctccgccgca gaataaggtg gaagaggcta
     8761 aaaaggttgt taagtatctg atagacaaac ataaggttga tataatatct attggaaatg
     8821 gaacggcttc ccgtgaaagt gaattatttg ttgcagaact tttaaaagaa atagatagaa
     8881 aagtacatta tattatagtt aatgaagctg gcgcatcggt ttattcagca tcaaaaattg
     8941 gtgctgaaga gttcccggat tttgatgtag cacagcgaag tgcggcctct attgccagaa
     9001 ggttacagga tccactggct gaactggtaa aaattgaccc aaaatcaatt ggtgtagggc
     9061 aatatcagca tgatatgaac cagaagaggc tcgatgaatc gttgaagggt gtagtagaag
     9121 acagtgttaa tagagttggc gttgatctga atacagcttc tccatctctg ctttcatatg
     9181 tatccgggat tagtgcaggt gtggcaaaaa atattgtaga atacagggag gaaaagggga
     9241 agtttaaagg cagacaggaa cttacaaagg taaaaagaat gggagacaaa acttttgaac
     9301 agtgtgcagg gtttttacgt atacctgaag gggagaatgt tctggataat actgcagtgc
     9361 atcctgaatc atacgaagcg gcattaaagc ttctcaaagc tatgggatat ggttttgagg
     9421 atgtgaaaaa cagaaaattg ggtgatatga aaaagaaaat agagcaaaaa ggtattgaga
     9481 atatcgctaa aaccttggat atcggagtgc ctaccctcag agatatagta gaagagcttt
     9541 tgaaaccagg gcgtgacccc agggatgaaa tgcctaagcc tgtattccat accgatgtta
     9601 tgaacataga agatttaaag gctggaatgt tgttgacggg tacagtaaga aatgtatccg
     9661 atttcggagc ttttgtagat attggtgtcc accaggatgg actggttcac atatcacaat
     9721 tgagtgaccg ctttattcgg aaccctatgg aggttgtatc tgtaggtgat atagtaaagg
     9781 ttagagtctt ggatgtggat gttgaaagaa aaaggatcag tttgagcatg aagggtattt
     9841 gaaacagaaa catgagcaga taagtgtgca gggcaagcaa aaaccatgct tagaaacatg
     9901 ggtagaaaca tgggtagaaa catggggacg ggttttttgt gccgttagta tttgcataaa
     9961 tacgctgtgg aggaatacat tatgtataaa ttactgattg ttgatgatga atatgaaata
    10021 agaaatggat tggttaaata tttcccatgg aacaaaatag gataccaagt ggtaggtcag
    10081 gctgaaaatg ggaaacaggc aattgatttt attaaaaaca atcatgtaga cataatgttg
    10141 tgtgatatta aattaccctt catgtccgga attgatgttg caagagagtt atataatatg
    10201 aaatccaaag tgaagattgt attccttagt gcccacaggg agttcgaata tgcaaaaaaa
    10261 gctcttgcct acggtgttaa ggattatgtt gtaaaatctg caacttatga tgagttgata
    10321 gatgcattta cttttgtaaa aaaggaactt gataatgata gagctttgaa aaatgcaaca
    10381 agcggggatg caacaagcgg ggatgcagca agagagtata gggacacagc aagtggggac
    10441 ggctcttgcc ttgcctttca gcaggcaagc aagaatcgtc cccacttgcc ttcagatgct
    10501 aatgatgaag gcattgatga ggcaaacatc aatcaggaag tcttttatgc aggtataaac
    10561 aagaaaataa tcaatacagt gaaagactat gttattaata actataaaga tgccaatctt
    10621 gaggatgcag ctgcacttgt ttatttgagc ccgcattatc tcagcaaata tttcaagcag
    10681 tatacaggtg tgaacttttc tgattatctt ttaatgataa gaatgaataa ggctgccgaa
    10741 ctattaaaag acattcagta caaaacatat gaagtaagtg aaatggtagg atattcaaat
    10801 tccaagaact ttacaagggc tttcaaaaac tattttggga aaactcccag agaatttagg
    10861 caaggggaaa acctggaaca agggaatatg gatttagatt agggtaaaaa tggagggata
    10921 ggaaaaatga gggaacgaat ttttttaaaa aatcttataa aatatcttat accaatgttc
    10981 gtacctgtca ttgttttcgg atccttgtcc atagccataa caggtaaaaa tataaaggaa
    11041 cagataaata agaacaattc aaatcttcta aaacaggcaa aagagaatat tgaaattatt
    11101 cttaatgatg ccgattcaat taatctaaat ctaatggctt atccagatat gattagtcaa
    11161 gttggtgaaa tactaaataa accaaatatg gaggctgatg attatattgc tataggcaac
    11221 acgagaaaga ttatagacgc ttccgctttt tcccggcctt atgtgaggtc tatctatata
    11281 tattttgaca atatgcaaaa aaggcttctt accacttctg aaggactcgt aaaaattgac
    11341 aactattatg ataagctctg gtttgatagt tatagcaaaa tggatacgag cagggaattc
    11401 tgggttgaag caagagaaat aaggcaatat gagtttgaaa aggacagaac tgatgttata
    11461 acgttatata gaaaactcta ctacacaagt gtaaaaaggg taggagcaat agttttgaac
    11521 ttttacaccg attacataga tcagttgtta agcaatcttg atatgtaccc cggtcaaatc
    11581 atcctgataa tggatgaaaa tgggaatgaa attttctctt attgcaatga agatgagaca
    11641 gtaggaacag gtaaaactga tataatggga agaacaggca taatgagcga agctggtaaa
    11701 atcagcaaag accaagagaa tgagaaatat gtggaaaatg aagaaaaaca agttgaaaca
    11761 gaggttaacc ttaaggaatt aaaagaaaac caatctccat tttttgttat tgaaattgat
    11821 aaagattcat atataattaa tcaactatat tctcccaaat ataatcttaa atacatttct
    11881 ataacgcctg gcaaatattt atatagtatt ccttctcaat taactctgat aactattttg
    11941 ctgctggtag catctttaat tataggaaca gttttaacac tggcttttag caggaaaaat
    12001 tttaatttta taaataacat aataactgtt tttgaaaagg ctgaaaaagg tgaaccactt
    12061 ccttcacaac ctgccaaaat taaagatgag tatagcttta ttatttacaa cattataaaa
    12121 gtgtttttag agcataatta tttgaaaatc cagctatcgg agagaaagta caagcttaag
    12181 gttatggaaa tgattgcttt gcattctcag ataaatcctc actttctatt taatacactg
    12241 aaaactgtat attggaaagc tattgatctt acaggggggc ataatgatgt aagtgaaatg
    12301 gtggagagcc tttcaacaat tattgattat tcattaaaca gttatgaaga atttgtaact
    12361 atgagtaaag aaattaataa taccaaaaaa tatattgata ttcagcaaat cagatataga
    12421 gataaattta acgttgtatg ggaatacagt aatgagattg aaaattataa gacaataaaa
    12481 cttatattgc agccattaat agaaaacagc atacatcatg gaataaacga aaaagaaggc
    12541 agatgccgga taagaattag gataactcag gttaataact gtttgaaaat aacagtaata
    12601 gacaatggta atggaattaa tcgggaaaat ttgatcaaaa taaggaaaag actatcacaa
    12661 tatgaatcgg ataaaaattc agtccgagaa tccggtaaaa atataggtct atataatact
    12721 aacagacgcc tccagttaac ttacggggag aaatatggct tacaaataaa cagtaaatat
    12781 ggcatgggga ctgttgtata tgttactata cctttagcag taacaacaga aacacaggga
    12841 cgatcctaaa ctatggatgg ttatgttgtt tcctctcttc gtatcttgat agaaacaaaa
    12901 gaaccgtcat catattacat gaccatattg catacccata ttacctaaga ccttttatca
    12961 tacaaaatga taccttttct taataaattg gtattttttg ataccttata gcaattcaaa
    13021 cgccttttat tatagtagct tcatttatat aatttatata tagaaaccaa cttcaatgtt
    13081 atatagatat tttgtgaaat cagatcactc aatattagct ataaggaacc actaatctat
    13141 ataataatgt atatccaaat tcatattata ataagaaaaa acatggtaat gtgttggata
    13201 tacttttatt aaaagataaa attaaaggga ggttttagtg tgaaaaaagt actaagaact
    13261 acatgtgtta ttattattgt tttaagtctt cttttaagca taactgcatg ctcaaagagt
    13321 ggagacggta aaaaacaaac tgacaacaat gtaactcaaa atcagggtac cgaaaaaagt
    13381 gttgaaccac ctaaaccggt tactataaga tttgcatggt ggggtggaga aacaaggcat
    13441 aaagctactg ttgatgcatt agagaagttc catgataaat atccgcatat tactgtacaa
    13501 cctgaatata gtggatggtc cggttacttt gaaaagcttt taacccagtt tgcctcgaaa
    13561 agtgcaccag atttaatcca aatttcgtac gccaatgcaa atgaatatgt tatgagagat
    13621 caactttatc cactaaatga ttttattaat gatggcgtgc taaaggttaa ggatcttcct
    13681 gaggtatctt tggatatgtt taaaattgat gataagtatt atgcagtacc ttcaggtata
    13741 atggctaatg catatttggt atataataaa gatatatttg acaaatatga agtaccatat
    13801 ccaagagaag gttttacctg ggacgattat tttgaaacag caagaaaact tactaaggat
    13861 actaatggag atggtaaaac agatatatgg ggaactggca atatctttac ggatgaacgc
    13921 gtatttttta aaatggttta tgagcagggt ggaaggttat tctcggatga cttgaaaaaa
    13981 gttaagttta ctggaccgga aggtgtagaa gtgtggactc ttgctaaaaa ccttcttaaa
    14041 gaaggaatag tagtaccacc tgaaatagaa gcctcaaatc ctccgggtgt agatagcttc
    14101 agaagtgaac aatgtgcttt agaagtaatt tatgcccccg caatgtatgc agacataaaa
    14161 ttcaactggg gtattgctaa gttacctaaa acagctgata ctgatattca ctggactgtt
    14221 ccatcaatga tatatgttat gagtaacttt agtaaggctc caaaggaaac ggccatgctt
    14281 ctggattaca tacttaatga tgaagaggca ggaaatattc taaagatgga aagaggagct
    14341 cctcctaata aaaatgttag gaataacatt gcaagttcat tgtctgaaac agataaaaat
    14401 gttatcagca taattgataa tgtagctcaa acaagtacat ttagcattga gccttaccct
    14461 cctggattta ttgaagtttt aaatttattg attagggaat ctcaacaagt aatgtatgat
    14521 aagaaaacac ctgaacaagc tatgaaggat ttagaaactg aagcaaacaa agtaatagaa
    14581 aagttcttta atgagaaata aacaataaag catttaataa tatatgtaat aaaaatactg
    14641 gcaaaataat aataaaatat taaaaataaa aattttttgt atagatgggg ttaagcgaaa
    14701 tataccccat ttatacatac ttgtaccgga ttggagaaag acaatgaaaa agaaattttc
    14761 aggtaaaaac ataaattcat tggaaagacc atttgaaggg tatttgttca ttgccccttg
    14821 gttgataggt tttttagcat tttcattatg gcctatgatt aattcagcga tacttgcttt
    14881 tgcagattat aatgtttttt caggaatgag gtttatagga cttgataatt tcagagtgat
    14941 gtttactgaa gataaaatgt ttggtcactc tttattaact acaactatat atgttgtagt
    15001 ttcagttcca gtacggctgg cagctgcttt gcttgtagct ttgttattaa atcagaaaat
    15061 cagaggaatg tctatttaca ggacaatata ttatttgcct tccgttcttg ctacaagtgt
    15121 agctatgctt gttacctgga aaatggtttt atcaagggaa ggaattgcaa acaaggtatt
    15181 aggttttata ggacttggtc catatgattt tctttctaat cctgatatag cactatggac
    15241 agtttcattt cttgctatat ggcagcttgg ctcagcgatg gttatattcc tggcaggttt
    15301 aaaagcaatt cctactgagc tatacgaagc agctgtagtt gatggaagct caagttggca
    15361 aagatttttt agaataacaa ttcccctatt aacaccaaca atatttttca atctggttat
    15421 ggggattatt ggtgccttcc aggtatttag cgtaggtttc gtaatgacca gaggaggtcc
    15481 tgcaaatgct acttatttct atgttcttca cctttacaga actgcatttg aaagcttcag
    15541 aatgggctat gcctctgctt tagcctgggt attagctata attgttatgg cacttacctt
    15601 attagttttc aggtcgtccg ataaatgggt tcactatgaa aattaataca tggaaaaata
    15661 atatatgaaa atcaacatat gaaatatacc ttatgagttc tttatgaact cctatgttaa
    15721 acaataattt ccatgttgaa tcttagaaag gatattacgc tatgagtgta aacactgttg
    15781 ctttaaaaca taatggaaaa caaagaacta aaaacggaag ggaacgtata gcaaaattaa
    15841 taatacattt gttcttaata atagggagtg tcattatgac atttcctctt atatggctta
    15901 taagtagtgc atttaaaccc agtgcagata tatttgatag aaacttcaga cttatacctg
    15961 aaacatttac ttttaaaaat tttgtaaatg gatggaacat caatcctcag ttttcatttg
    16021 gacacttttt tatgaatact gttgtattga caattttagt tgtcataggg actgctattt
    16081 catcatccct tacagctttt ggttttgcaa gacttaagtt caaacataaa aaactattat
    16141 tctcaattat gttatcaaca atgatgatac cgggacaggt tacgcttatt cctcaatacc
    16201 ttatatttaa tagaatcgga tggctgaata gctatctgcc aatggtggtg cctgcgtttt
    16261 ttggaggcgg ggcgtttaat atatttctgt taattcagtt tataaggggg attccaaaag
    16321 aacttgatga atcagcgagg atagaagggt gcagcagctt tagagtatat tggaatatag
    16381 ttatgcctct gtgcaaacca gcattattta cagtagtaat attcactttc cttggtactt
    16441 ggaatgattt tatgggacag ttgatatacc tgaattctac agaaaaattt acagtttcat
    16501 taatacttaa ctcactgatt gatattactt cagcggtaag ctggggagca gtaatcgctc
    16561 ttactttatt atcagtaatg ccttgcatac ttttattctt tgtattacag ccatattttg
    16621 ttgaaggtat tgccactacg gggttaaaag gataaattat gtagtagcaa gaaccatcgc
    16681 ctctcctctg gctctacttg tccttctaat atgagagtga tatactaaaa aagggtaaac
    16741 tatgtgtaaa attttatttt tggaaaggac gtgctattat gcagcttctg gtattagtgc
    16801 ttaacaaagt agagttgctt gacgagttac taaaaagttt tgttagtgcc ggcattaagg
    16861 gtgcaaccat ccttgacagc atgggtatgg caagagcact gacttccggt gggaatgaag
    16921 atatcccgat ttttggatca ttaagactga ttctgaatga aggacatccc tataataaaa
    16981 ccttatttgt tgtacttaaa gatgatgagg ttaatatttg tgtcaggtgt atacgggaag
    17041 ttgtcgggga tctgaacaag ccagatgttg ggattttatt cacagtacct gtgaatttta
    17101 ttgaaggtct tactaaaaag aaccaggagg gcaaatagga aaaaacatca atgaacacat
    17161 tattttatgt atcaatagtt ctgttagcgg ggcttttaac tgctaagctt acatcccttg
    17221 ccaaattgcc taatgtaaca ggttatttgg tgggggggtt aattataggc cctagtattc
    17281 taagagttgt tccaaaagat gcagtaacac atatgggaat tatatctgaa gcggctttag
    17341 gaatgatagc atttagtatt ggcagtgagt tcagttttaa gcatttaaaa caacttggtt
    17401 ccggaattat tactctcact gtaatacaag ctttatcagc ttttgctgct attgtgactg
    17461 caatagtatt aattttcggc aagagtatag aggtaggtct ggtgcttgga gctatcggag
    17521 tagcaaccgc acctgcagcg actttacttg ttgtaagaca gtataaggcc agtggcccgg
    17581 ttgtagacac actattaccg gttgtggcta ttgatgatgc tgttggtatt atggtatttg
    17641 gactttgcat ggctgtagcg aaggctgtac atatgcccga acagaatgtt tcactggcaa
    17701 tgtcaattat taagccaata tgggaaatag ggctggcagt tattattggc tttctccttg
    17761 ggctgctatt atctttcatt gcgaccaaag taaggggaga tgaagaatta cttggtatat
    17821 cacttgctat ggtttttatg ggagtagccc tggcaggtaa atttggcgtg tcatcattgc
    17881 tattatgtat gactatgggt ggagtgctaa ccaaccttgc atctaattca cagaaggtat
    17941 ttagtatggt tgacagattt acacctccga tatatgtagc tttttttacg ctgtcaggag
    18001 ctgatctgga cttaagcata ctgaaaagcg taggtctatt aggaataggc tacataattt
    18061 caaggtcaat aggaaaaatt gtaggagcat atgttggagc taaaattatc aaggcccctc
    18121 ctacagtgca gaaatatctg ggaataaccc ttctgcccca ggcaggagtt gctataggat
    18181 tatctctggt agctcagagt gttttacctg aatttggagc atctattaga actgttgtat
    18241 tatttgcaac actaatatat gagcttgtag ggcctgtact tactaagata gccttaacag
    18301 gagccggtga aatacagcct gtgaaaagca aggcacagct tacacggtaa atttcagaca
    18361 aaggctgtgc ataatgtgga atcacaggta tatgtgcaga tgaatacgcg cgaatgtaca
    18421 ggtaaggcag gaataattcc tgcctttttc cgtttctctt tatccatagt gtcaagaagt
    18481 ctctcactta tgccaatgtt actttgtgct cttctgcata ataatttggt ctgtctgcag
    18541 ttttatttcc aaatattgag cgctttcagc ttttgcggga gagataacaa tgtcaaaatt
    18601 tgtccattca ccaggacgga ggacatttga aacagaaaag tcagcactgc ttaatgactt
    18661 atcccaatca tcatgattta tagatttatc ttcacgatca tattttacac cccacctgac
    18721 ttttagtttg cagccattgt aaacgcagtt ctgaggattg cctaatctga aatacaattt
    18781 ataaccttct ccttgtagtt ctgctttatc taatattaaa aagaacatgc ctgtacttgt
    18841 atctattctt gcgaagccct tacctgattc agaaacatta aaaactgctt tattgaattg
    18901 atcaagcatt agtttggtaa gagctaaatc tgtcatcgtt tttattttaa actcattgta
    18961 atctttcagt tctggaattt cgctcgtgtt gttgttatcc ttactcttat atttgtttaa
    19021 atccgctgtt aatgtttcta tttcaccttt caatccagaa attgccttat tcattgcaac
    19081 ataattatac actccaaata tggatgtaaa aattaataat acactaaagg caataatcag
    19141 gatttttttt attaaagcca cattatttcg tataggtcct attacttttt ctaaatttaa
    19201 ttgtaacact ttttactcca cctcacaaaa tgcttatttt tttgatgata aaaattggtt
    19261 tcaaatatta aatagtactt taatactata aataacgact aaataacgtc taaataatat
    19321 cagaccttat ttatcggtaa attataaatc gctctactaa tattataaat tagcctacta
    19381 ctattctaca attaaacagt gttattatgc aaactatttc ttaaaatctt ctaaaaatat
    19441 taactcattg ataatgtaca gaatagacaa aaataatatg ttattttaat aaatttttaa
    19501 caaaaacatt gaatcttttt gctaaaatag catgtataat ataaaatgga ttttattttt
    19561 aacataaggg gaaatgattc atgaagggaa atacaatttt aattaatata ttaatgatgg
    19621 ctgtaatttt tgtgctggct ttttcttttg ttgctgtatt aaggcataca ttggctgata
    19681 acaatatatt cgctgaagta gcatcggaga cctccttgaa aattactgag aagcaaataa
    19741 aggaaacgaa aggcaacctt gatatggaat ttagcattcc tgttattgaa gggcttgcag
    19801 ataaggaaat acagtcaagc attaattcta tgctggaaaa tgatatagaa tcttttagaa
    19861 agtatatgat tgaactttct gctacagata cagatagcag tattaatatg aaatatatag
    19921 ctaagacaag ctataaaatt aataaaaaaa gcagaaacct gttaagtatt tctgtaatct
    19981 actatcagga caccggtgga gctcatggca tttatgacat ggttgcgtat aactttgaca
    20041 ttcgaaatgg ttctttattg gaacttgatg atctatttga aaataaggat gggtataaag
    20101 atattattaa tgaagaaata agaaggcaga tatgtgaaaa taagggaaag atcgagtact
    20161 ttagtggtga tagtggcttc aaatcaattt ccagccatca gcctttctat attaatgatg
    20221 gtaaactggt aatttatttt agtttatatg aaatagcacc ttattcatct ggaatacccg
    20281 ggtttgagat accatttaca ttatttgaag aaaatctaaa cgagattata gcacagtgaa
    20341 gtcaaattaa aaattgatta tttgtgcaat atgttaattg aagtctttgt taatatatgt
    20401 tatatataat agggtaaatt acgaaaatgt tgaatagcaa aataaaatcc taggggggtg
    20461 caggttacat gagtatgttt gaaatgctta tgcttttgtg ttttggagca gcttggccgg
    20521 tatctatata taaatcatat acatcaagaa agaatagtgg aaaaagtctg ggtttcctta
    20581 ttattatcct tataggatat atatcgggag ttattcataa gattttgtat agccgtgatc
    20641 ccgttatcgt gctttatatt ctaaatagta taatggtttc aatagatatt gcattatact
    20701 tcaggaataa aaaagcttca aattaaaagg acctgtactc tttgcagtaa atttgcagta
    20761 tattaaatgc aaaaccaggc gaaaaagaag caagcattat ttaaacagct tgcttctata
    20821 tttttcaagt tgcatcatta agatataacc tacaccatag caggcaacag cttgtccaag
    20881 agcaacccaa cccattgcaa caagtacaga taccttcaga atataactta acatgattcc
    20941 tactaccact gcattaacca gcaccggagg taatggtaca agataccgtt taggcatttt
    21001 ataggacaat atagctgcaa ttaatgtggc aagactgcca aaaattatat ctaaaggtcc
    21061 attccctcca ataatattgg aaattatgca tccgataaaa aggcccggaa ttgcagcggg
    21121 tgtaaatgca ggcaatactg taagagcttc ggcaattcta acttggaaca atccataact
    21181 tataggtgaa aacacgattg tcaggactgc ataaattgct cctataacag ctgcttcagt
    21241 aataaatctg aggctcttgt tttttttatt cacaaattta tacataaaac atccctttca
    21301 gcaagatcca tcttataata attattataa gatgatcaac tttaaacttc ataattatta
    21361 tttgcagttg tcagcagtaa taactgtatt tatacaaata attatgcaag taattacgca
    21421 aattaacatg gaaataatca tatcatcgtt tattgcattt ttcaataagc tcagttaata
    21481 tatctatata tgcttaatac tactatgtca atttagagag cgatgtcttc tgtgatattt
    21541 ttttgttgtt cttcggcaaa acacataata ataaagtagt attaaataaa gcaatattaa
    21601 atgatattaa tagaaattcc taaaggaagg ggtttatata aaatgattga aaattcaaaa
    21661 gtagcagtat tggagacaac tcctgagacc gtaatcgaag attacaagaa gcttatgagg
    21721 atgtcggatt atgataagta tttaaaaaag gacaggccaa ccatacttaa aaataacata
    21781 tcatggcatt tcccttatcc gggagcaaac actactccct ggcagatgga ggcagttata
    21841 cagtgcctgc gtgaagatgg ctataaagat atagtttgtg ttgaaaataa aactgttgta
    21901 acaaatgcgt ataaaggtga aagacttaac aaatatgttg atatatttaa aaagtataat
    21961 atacctgttt tatataactt caaggaagag gatatgaaat gggttaaata tgaaccaaaa
    22021 ggggaaatac tggtacttga caagattttc ccggatggca tatatatacc tgattatttt
    22081 tttgataaaa atataataca tctgcctacc atgaagtgtc acatttatgc cacaacaaca
    22141 ggagcaatga aaaatgcttt cggaggtctg ctgaacacaa aaagacacta ctgtcacagt
    22201 gaaatccata aaacacttgt agaccttctt accatacaaa aggaaattca ctctgggata
    22261 tttgcagtag ttgatggaac tgtatcggga aatgggcctg gcccaagaac tatgaagccg
    22321 gctgttacaa attatatatt ggcaggggga gatcaggtcg ctgttgatgc ggtatcctca
    22381 aaaattatgg gatttgaccc aatgagcctt gaatacatta gagaaggaaa caatgcaggt
    22441 ctgggcaatg gaaggatgga gaatattgaa gtagttggtg aagatatttc aaatgtaaat
    22501 tggggatata cagtaggaga taatacagca agcaaagtag gggatctgtt atggtttgga
    22561 ccgttaaaaa agctccagag acttatgttc catactccaa tagttaatat ctttatttgg
    22621 ggttcggcat tctatcatga tgttatatgg tataatttgc aaggtaaaaa agtggtaaaa
    22681 gattatcaga ataataccca atggggcagg ctttttaagt cgtataaata gggaaaccaa
    22741 actgcttttc aagtacagct tgttacagat ggtcatttgt ggatcgatgc attatcaaat
    22801 gtggatcgat gcattatcaa atctagctca tgaagagcta aagaaacatg ttgaaagggt
    22861 aggaagagtt atttatactt ccaacctctt attcctggaa ccgttccttt gtttcgttgt
    22921 tttattattc ctccaaaagc tgcatacaga ataacagggc tcttggcaga tgaaatggtc
    22981 ctttccacat ggagcccttt aaagtatgcg atacagttcc atctctatgt agatatccgt
    23041 accattcgcc atattcctta tccgggaacc tgctgaaagc ccaactgtga accttttcat
    23101 accaatccat atattcctgt ttccccgtat atttatgtgc aagaagaagg gcatagagag
    23161 cttctgtatg aggccaccat agtttcatat cccattcaag ctgttcctcc ggtcttccgt
    23221 ctatatccac aaaatataaa attccgccgt attccttatc ccagccaagg tccagtgacc
    23281 agttcaatat atccagggct ttctgcataa gggcatcatc cttttggtac tttgcttctt
    23341 tcatcaggaa ccatgatgct tctattgagt gcccggggtt tatgcacctt cctgcaggag
    23401 tatccaatct ttcaccattt aagcctactg tttcaaagag agcttttact tccggtttta
    23461 ggaaatcttt tagtatttca tctacaagtc tctttgcgat atgatcatat gcatttcctg
    23521 catttcccat gtctgtttct cttaagatat agttggtatt cagtaatatc ataggaacag
    23581 ccatggcttt tgatgctctt gtttctgcat agaccttggg tgttatctta tatgggtcat
    23641 ttgcagaatt ttcataaatt gacaatacca ttttatatat gcttttagcc ttatcaagag
    23701 cagctgtatt acctgtagtt ttgtaatatt ctgccagagc aattatagca aaggtttcac
    23761 taaacaagta acgccttttc ctaagaggtt tcccgtcctg tgttacctgg aagaacatac
    23821 ggccatcatc atcaaaacaa tgcttcataa taaaattata tcccaggtgg gctgcttcaa
    23881 gccattcatc ttttttaccc aaagtattgc agagccttga gtaaatccac aatcccctgc
    23941 cttgaaacca aacagattta tctgtattga agactttccc tgtccggtca acactagtaa
    24001 gataaccacc gtatttgtgg tcaagagaat gatttagcca aaatggtata caatcatcca
    24061 ataaagagct tttgtagaat ttgtaaagct cattttttcg gtttttatcc atattatttt
    24121 acctccaaag acgttttgat gtagtaagta gtatttgaca ccataaatat cattaattat
    24181 attttacaca ttaaatataa ttcctgcaat tatatttatg gtgtcaccgc agatcctggc
    24241 ataattaaat ccggcatcta ttttacctaa aaaacaaatt catttttctc agtaagttcc
    24301 tttgactttt ctattaactc actcagaatg tcaatcatac tttctgaaag ctttttagcc
    24361 tcttctgtcg cttttatggt agcttcacgg ttaccggcat ttatactgtt tattactaca
    24421 ttgcctgtgt tatgaaaccc gtgatgtata tcctctatct tattccaact atttaatatt
    24481 tcaggatgag aaggctttac agagtagtag aaatgaccga aactgcagtt atggtcattt
    24541 gtctgaagag gtcttatttc catggcttcg accatagatt caagctctga aacccacctt
    24601 ttatggttct taattgcttc ttcaataaac tctgaaaatt tctcgttggg aatgctgtat
    24661 aatctgtcaa gtataatttc cccacacttc tttgttgcat catccaaatt attttccagt
    24721 atcaacatag cttcagataa gttttccata tccttgccag tagattccag tgaagatgaa
    24781 tggatgcgaa gttctccgga cattagggta agactgttca tgaaatttgt aagttcctca
    24841 agagatgatg taatttcttc gcttacagca cttatatttt ccaggcttga tgcaatacct
    24901 gtaatggatt ggttgctgga ttctattgac gacatcattt ttgcaatgga agtattaata
    24961 tttgtcaggg aatcggcagt atgttcaatg ctttcggaac ttttagccga attattatat
    25021 atatcattta caatattgcc tatagatgta agcagatttt ttgttgagtc agatagtttg
    25081 cggatctctt cagctactac ggagaatccc ctgccggctt ctccagccct tgcagcttca
    25141 atagatgcat taaaagcaag aaggtttgtc tgatctgata tttcataaat tccatcaaca
    25201 atctctttca tactatcaag ggattcctgc aggattttaa aatcatcttt catagattga
    25261 gagtatacga gagcattttt agtatctata gaaatctgat taactacatt ccgattatcg
    25321 gtcgtgttct ccttgagctg atttgctctt aatgtgatgt tttcaatgga gtatgcaatt
    25381 tcagtattgg catttgttat ttcggttgct gaagcaaggg aactctctga tgacttatgc
    25441 acatcaaatg acatattatt tatttttgaa cctatattat taactttttc accgaagaat
    25501 ttcattttca aatcgaaact gcttgttaaa aaagcagcct taaaaatatc ttcaaagata
    25561 gatacaagtt tttccctgcc tttttcgaga cggtcaaaaa ttaatggaat atccctttgc
    25621 caattacctt taaacttttt gtttacattg taattttgat cattatttgt acattccatc
    25681 gcgcggatta ttttctggat cggttcatat ttttttctac tataaatcat tttctacacc
    25741 ccttattatt tatcggatta acagttaaca actttatata aaaatgtaaa aaaaacgaga
    25801 taaatagttc tgtatgttta taataactct atcgactttt ttcgctattt ttttcaaaaa
    25861 atattttaaa taatataagg ctgtggtaaa atgaaataag atacaataaa acaatataag
    25921 tgatattgaa tcatttatat gcatgccaaa atatagaaaa gggggatata aggccatgtg
    25981 gaaagaattt aaacaattcg cagtaaaagg gaacataatt gatatggcaa tgggtataat
    26041 tatcggttct gcatttagta aaattgtaag ctctctggta aatgatatga ttatgccgat
    26101 tttcagtttg attattggaa gaattgattt caccaaccta tttatagcac tggatggcaa
    26161 acactatgag aagcttgggg atgcaaaaga agctgcagtt tcaacaatta attatggaat
    26221 gtttatatca gctatcattg attttttaat tattgcattt tccttattcc tgataataag
    26281 aaatgttaat aaattaaaaa agaaagaaga ggcaccgcct gcagagccta cagcaaaggt
    26341 ttgtccatat tgccaaagca ctatatcaat aaaagctaca aggtgccctc aatgtacctc
    26401 tgagcttgta gaaacaatga aggtttgatt tttaagtata tatgaggtta caggatatac
    26461 ttgactattt tatgggtgta tcctgttgct ttatgcaata tcaattcaat gtaaaatcaa
    26521 catcattagc caaaacctgg tatttcttaa tgtatttaga tacaggctcc attatttacc
    26581 tccgttttgt ctctccgttc tgactctccg ttttggctct ccattttggc tctctattct
    26641 ggcttccatt ttgcagctat acacctgcat acaatttcaa aaagccaaca aattatagct
    26701 gaattaaatt atttagtaac aggcactggt taattataac actaattttg caaaaagtta
    26761 acagtttacg gaaaatatca acatatttaa actatatcaa tacttttgaa tagacaaata
    26821 aattctatta tgatagctta tatttgcagg gaaaagttgc agcaagcttt aatatgatta
    26881 cagggggaat atataaatgg atttcaacag taacactaaa atcatagata gtcatgtgca
    26941 tatcagaagt ttaaagagta tggaatttct ctataattat ttaaaaaact ataacctgaa
    27001 aaaaataaat attgtaagtt tggcatgtaa agatcaaagt gaaatagcgc aaaatctttt
    27061 atgtgcgtta tttaaactgc tgcatcctaa aagtgtatat gcttacggct cattaatgta
    27121 cccgtactac ccgctggatg aaaaaatacc gtataattat aaccttgttg agcaggttaa
    27181 aacattaatg aatatggggt tcgacggcat taagatgctg gaaggaaagc ctaccttgag
    27241 gaagaagata ggtataccac tggattctcc catttttgat gattactatt catatcttga
    27301 agaacaagag ataccaatag tattccatac agcagatccc gaaactttct gggacaggga
    27361 aactgcacct gaattctctt ttgagcaggg ttggttttat ggtgatggca cattcccggg
    27421 aaaagagact atatacaatg aaattgacgg gatattgagt aaatttccaa agttaagggt
    27481 aacctttgct catttttatt ttctatcaaa ttacatagag agagcggaag catttcttaa
    27541 taagtggaaa aatgtaagcc ttgatattac gccgggtcgt gaaatgtatg ataatttctc
    27601 aaggaaacca tcagaatgga aagctttctt tactaaatat caggacagga ttgtctttgg
    27661 aactgatatg gaagacagta tgttccaggg aggtccttca gatataataa atacaattag
    27721 gagatttctt gagacgggtg ataaatttca taattggggt tttgaaataa acggactggc
    27781 ccttgataaa aatgttattg acaaaattta ttataaaaac tttgaatcct atgctggaag
    27841 caatccaaaa atggtaaata ctaaaagact gcttgatgag tgcaggagaa taaagggtat
    27901 agcactagaa agtccggctt taagcacagt agttcctgaa ataaataaaa taattgaaaa
    27961 tataacgagt cgatagatgt cccccacctc tactgatgcg ttataaaata aaccgattat
    28021 aggctattat attaataccg gataatattg aaatatattt cgtatatagg atcatattat
    28081 tgcattttga tgtaaaacac ataattaact aggattctaa ggagacgaca gaaatggata
    28141 aaaatgataa aaaaagcaat acacttaaat ttaaaaaata tttaaactct ctttcaataa
    28201 agtttattgt ttgtactgct gcagttacac ttatttcagt tttgtccata ggattatttt
    28261 cttttttgta tatcagcaat agcataaatg aggaaattac acgttataat actcagcgtt
    28321 tgaactatgt taaggaaaca gtagacaata gggtgtttgg caaaacgctt aatattctat
    28381 ccgggctttt ggtaaagaac gagtttaatg aattgttata ttattacctg cagcatccaa
    28441 aaaccaataa tttaactaag gttaactcag ttatgacaat tctaaaagat aaagcactta
    28501 caaacagcga tatattatct tcagtatcaa tttattataa aaacaacgat attgtaatat
    28561 cctcatcaag tggaattaaa tatctgaatg atacaaataa gaatctgcct ttcgatgatg
    28621 agtggatcga tatttttgat gatatgagaa aaagcaataa aggaaggaat caaaatgtta
    28681 tatggattcc cacaagagaa actccatgtt catatggcta cacagcaaat gaatataata
    28741 tatcacttat tggaaagttt ccgcaaaatt atgtccagga gggttatgat tccttagtgt
    28801 gtttgaatat cagcgaaaaa gtataaggaa tacaataaat gatattgtaa aagatgatga
    28861 aataaatgtt ttaatagttg acagcaaagg caatgtaatg tctgataata atggaaaaag
    28921 tatttgctca gatataagta atagtggtat ttttaatgtt gtgaaccagg attccggaga
    28981 gcatgatata gagactaaat cattgataga aattgatgga atagaaactg taatttcatc
    29041 agtaaaatca gattactgcg gctggtatta cattcttatg gctccctcgt ctgtatactt
    29101 tcaaaaaacg gttattgcaa gaaagataat attcacatta tgtgtaataa tattttttgt
    29161 tttacttttt gtagtaatgt tgatttctgt aaaactaaca taccccttta acaggatatt
    29221 aacaaatgca aaaaatgtgt tgggtgaaaa gcgcagtgaa gtttatagaa atgaattatt
    29281 atgcattaat gatgcattaa acatgctgtc gcaaaaaaca aatgttcagg aacagatttt
    29341 aaatggcaat agagatttgg taaggcagac tttcttttta aatttaatga gtgggcagaa
    29401 atttaatgat aatgatatag aaaagacact ggaattttta gaccaggaca tgagtcttcc
    29461 ttacttcaca gttgcaatca tacagaataa tatgtttgct aaagctgatt ccctgtttaa
    29521 taaaattgaa aatgatatat accattatat agataataga gaatatgatt atataaatag
    29581 ggatttgaat attatttata taaaatacaa tgagcatcat atatcactga ttattaacca
    29641 cttaatacct tcacctaaaa ttgctgctga ttttgttaat aacttaaaag aatacattga
    29701 aaaaagattt gatgcgatcc tgcattacag tattggaact gtttgcaata aaattaatca
    29761 gattgacaga tcattttacg acgcttatga tgcattagcc tactcatcca tttatcccac
    29821 aataaggatt tttgagcatg atataaccaa gaaatgggat aagaacaata acttagagta
    29881 cggatatatt gatagctttg ttaaaagtct gaacgaatat agactggaca actgcaaaca
    29941 atatttgtat attattatga acaatgtgat tgaggaacat ttatcatata aaacatataa
    30001 aagtattatt aatgaaataa tactatcaat agaaaaactt gtaataaaca ctaaaattga
    30061 cctgagcgaa atatttagtg tttcaatagc tgaacaggta gaatctcaat actatattat
    30121 tgatgtattc aaaactattg aatgtatatt gggtaaaatt cttgatcata ttgtatctca
    30181 gcattccagt cttaattctg tatatgtggc agaggcaaaa aagttcatag atgagtatta
    30241 ttataaggac atttccattc aggatatagc tgataaactg aatatcagca gacaccatct
    30301 gtgccgtgtt ttcaaagaaa ccaccgctaa cactctttta aattacatta ctgaagtaag
    30361 gatgaataaa gccagggagt tattagctga aggcaaactc ccaataaatg atattgcaaa
    30421 ggaaattggc tttaataaca ctacttattt taataaaaaa ttcaagcaaa atttcggtgt
    30481 tacaccaaca cagtatcaga gtaatgttag ctgtgaaaag acggtatagt accttaatag
    30541 atatcaatat cattcaagca tatcaataaa gtttaatatg tattaatcaa tacatttgaa
    30601 ttatatcaat tattttgaat agacaactgc gtaaatcgta tgttagcatt gagtcataaa
    30661 atataccggg caaaaaatgg tataaagtta ttattaaaga aagaggcgag attgtgaacc
    30721 cttcaaaagt tactgcttca gcttcagata ttaaatatgg aaagaagctt tcaggtaaaa
    30781 actcaaaatc ttgtaaaaat tcaaaagtct ctaaatattt cagcgaatgt gccgataact
    30841 atcagcttgt tctaatgttt ttacctgtta ttatatgcct ggcattgttt agttatgcac
    30901 caatgtacgg ccttgtcatt gcatttaaag attttcaact gctaaagggt atttcaggaa
    30961 gtgaatgggt aggtcttgag aacttcagaa agctgcttat cacgcccagt tttcatgaag
    31021 tgtttagaaa tacagtagta ataagtttac taagaatact atttgggttt cctgctccaa
    31081 ttattttgtc tatattactt aatgaaatat atcataatac ctataaaaaa atagtgcaga
    31141 cgataagcta tctgccccac tttctttcat gggttgttct gggtggatta ttcatgcagg
    31201 tgctatcacc ttccggtggg ttagtaaatc aggtaataac tgcatttgga gggaagccca
    31261 tatattttct ggcagatccg aattatttta taggtatgct gatagctaca ggaatatggc
    31321 aaagtgcagg ctggggaaca attatatacc tagctgcaat aacatcaatt aatactgagc
    31381 tgtacgaagc agctgttgtt gatggtgcta ccagatttaa gcaaataata catattacct
    31441 tgccatcaat agcacctact attaccatat tgttcatact gcagatggga aatattgtaa
    31501 acgctggttt cgatcagatt tataatcttt acaaccctgc tgtatataaa gtagcagata
    31561 ttattgatac atatatgtac agaaaaggtc tcattagcat gcaatatagt tttgccagtg
    31621 ctgcaggttt atttaaaaac attatctcat tcattcttgt attccttaca aactgcgttg
    31681 taaagaagtt taatgaatac tcgatttggt aaataaaaat ctgcaaataa aaaatatggt
    31741 gaaattacaa acacttttat atactgaaag gatgagctta gcatgtcacg catattaaaa
    31801 aaaacattgg gggacaagat ttttgacgta gtaaattata tatttatgtt tatattttgc
    31861 cttacaatat tactcccttt tattcatctt cttgcacagt cctttgacaa ggtgggtaac
    31921 atttctgctg aaggtttata catatggccg gaagagttta cctttgataa ttataaaatg
    31981 attgcaaaaa acaggtatat atggaatggt tattataata cattttttag aacaattgtt
    32041 ggtaccggta tgtcattatt ctttacatca atgggagcct atgcattatc gaaaaaaaga
    32101 tttccaaaca ggacattctg gacaatggta gttgtgttta caatgttttt cagcggcgga
    32161 cttatcccaa cttttctctg gataaagaaa ctgggacttc ttgataagag aatggttctt
    32221 atactacctg gtcttattgg agcatataac ctggtactaa ttaggaactt ttttcagcag
    32281 attccgggag aactcgaaga atcagcaaga attgatggtg ccagggattt tacagtattc
    32341 ttaagaataa tacttcctgt ttcaaagccg attctggcaa ctgttgcttt atggctggca
    32401 gtagggcact ggaatgcgtg gtttgattgt cttgtttata taaaggatac taaaaaatac
    32461 gtagtgcagg taatacttca gagaataatt ctacagggta ctcaacagat gatgatggat
    32521 gatacaatgg ttcaaaaaga gtatgaaagc agacctgatg taataaaagc tgcgtgtatt
    32581 tactttacta caataccgat actctgtgtt tacccattcc ttcagaaata ttttgttaaa
    32641 ggagttttca ttggttcgtt aaaaggctga agcaggcaaa caaggttaaa aagccttgtc
    32701 aatatatcaa acttaatgat atcaaactta aatgctcagg attatttaat gtaagtatgt
    32761 ctttaagtac ctgactaagc agaaaaaaat tagtgcaaat aattaataca aggttgcttt
    32821 tacaggtgct ttagtatata aaaaatatag aaaggggaat attttaatga aaactgcaaa
    32881 aaaaatcact gctttaatac tttccgttat ctttacagta gctcttctgg caggctgtgg
    32941 aggaaagaag aatggcaatg cagcagaacc aacaaaaaag gggacaactg gtactacaga
    33001 aaaagcaggt agtaatactg acaataaaca ggaagagaca tatacataca caataataca
    33061 atggcttcct gcaccactag acgacaatgc tccaatggta aaaaaatggg aagatgagtt
    33121 tggggtaaag ttcaaatttg actatattga gacaactaaa attactgaac ttttaccctt
    33181 gagaattgca tccggagata ttcctgatat aattacaagc cttgactata atggctttta
    33241 tgattatgta aaacagggaa tagccggtac ttgggatgag gagtttttca gaaaaaacgc
    33301 ttcttatatt tcaaaattaa tggatgaaat gggggaagat tcctggaaaa taagcaagta
    33361 taacgagaaa atgtttactg tacccggtat taattgtgaa tatatgtatg gagcggcaat
    33421 gatatggcga aatgactggc ttaaaaatct gggaataaca gaaattccat ataagcttga
    33481 tcaagtagag gctgcattgt acaagttcag aaatggtgat cctgatggaa acaagcaaaa
    33541 agacacatat ggactctctc aggatggcat gaaaatgata ttgggcgctt ttggcgctcc
    33601 ggaaaaaaat tggattgatg atggaaatgg aggagtcatt tattcaagta tagcacccca
    33661 gacaaaagat gccctggtat tgttaagaaa atggtataaa gacgaagttc ttgatcctga
    33721 atttataact ggtgagaaca agggcgggta ttgggcctta agtcatgctt tctgcaatgg
    33781 aagaataggt ttcacggcaa gagctgtaaa tgctcactgg aaagacccta aactttggga
    33841 attaggagag gcgccatatt taaaagaggt aaaagcaaca agattaccta tgcccgaagg
    33901 aaaaacaatg cagataagcg aggataaaac cgaccttaat gatcctacta tttaccttac
    33961 agcacttgga acggatataa agggctacat tgcatatctt acaggagata cgcaaatgct
    34021 tgaagactat tttactgtgt ggcaaaagtt acttgaaatg gaagttaacc atcttgtgct
    34081 ttataaaaaa gctgttgtat ggcgaatact ggaaatgact ggtatgcttc ctgaagaact
    34141 caggcagcca ttactaaatt ttttttataa atgggcaaac agcgaggaag gtataggttc
    34201 tatagatcac agtttatatc aatcacctta ttatccgaga cagaaccatg ggttaatacc
    34261 tgctctggga cttgtatatc tttctgatta tttcacaaag ttccatgcgg atttaaagga
    34321 accctatata tggaaggaaa aagctgataa agtatatgct ccatatttta gtgggagctg
    34381 gaaacccgta tgtgatggcc tttgccatgg ttggtggctt tcacaaccag caatgcttga
    34441 atatgggctc atggatgaca aacataggta ttttgagggc ggcggagcac gtaaggctgc
    34501 agaatgtgct atgaccgtag ttaatagtga aggctggatg ccatgctcag gtgatagtga
    34561 tattttgagg caattccctg gatttagttt aaggattgca gctgcttatt ataatgatgg
    34621 aaggtatagg tttgttaatg aaatggcccc tttcgcacag aggggatatt gcggtccaat
    34681 tacatatcct ccccgcgcat tcgatacagg tgtgcaaaaa gaagaacctt gcgataaaat
    34741 cggcatcgat gttattccta ttgatccact tgtatattac gcctgggaga aagaacctaa
    34801 attagcttca ggtgcatctg atgctcctcc taaagctccc atagataaat gctttgataa
    34861 gctggctata cgtacaggct ggggaaggga tgatgattat ctgttgatag atggccttgg
    34921 aggaggatcc cactcgtatg cagatgctat gagtatactg gattaccaga gatttggggt
    34981 gagttttatt gtggcagaag actccttgca ctggccggag cctgaaaacc attcaatggt
    35041 taccatttac aaagatggca aaaaggaaaa aatcccaagc tttgctgagt tgatggagac
    35101 cgttactgat ggtaaaggga atatgtatgc taagatgaag ttgaaggatt ataatggcgc
    35161 agactggata agagaaattt acatgatacc tgaaaaatgt gtagttttcc atgatactat
    35221 aatagcaaat gattttggaa attacacaat agagaaccat tttaggacac caggcattgc
    35281 agaatttgaa aatactgcag gaaatattat tagaagcaaa agaaaaacac ggaatggaga
    35341 aactgtggaa tttaaacttc ttagtaagtg cagcaggaat tcaacatata gcatggaaaa
    35401 gattcctata gacttgaaat atagggtttt acccggaagt gaacttcctt tctctgaaga
    35461 aaccaatgat gctgcagcag gaaggaaacg ttaccattta agagatatct gtcttagtgt
    35521 atttactgca agaaccgcag taaaacttaa taagggtgaa caggttaaat ttactcatgt
    35581 tgcatgcgct gataagaatt ctgtaaatat agaaatactg gaaaatagca taaaaatgtt
    35641 ggaaaataac agcaaaatat tggagaatga acactcatat aaggaatctc gaaaaccggt
    35701 aaatataaat ttaaaaatcg gcgatgaaat atatactttg ccatttagcc ttgaatcaat
    35761 aaatgcagga aatgttgata atgttggaga tgttggagat gttggaaatg taggaagcac
    35821 aactaatatt tccatatccg aaataaaaag aggatatgaa ataaaaactg tatgtgagga
    35881 aagctcagcc ataactgcag cacaattagt gcaggataag tatttgctgt gtggtttgaa
    35941 agatggaacg gtaactatgt atgatgtaga aggtaatact atatggtctt ctgaagttga
    36001 tggtaaaata catgatgttt ccttgcttga ggataaaggc accaaaactg ttttcgcagc
    36061 acatgggaaa gcaggtataa ctgctgttaa tgaaaaagga gaagtactat ggagtaaaaa
    36121 aataacaaga attcctacat tatatccctg gtgggagttc gatgatccaa cacctgttaa
    36181 aatcaagact ggcatactaa acggggaagg attggctctt atagggtgtg gtgataacta
    36241 tgtaagattc tatgataaag atggaaatat gaaagatgct tattactact ttgcatcagt
    36301 acctgcatat attcatatat ttgatgttga tagtgatggt gagcaggaag ttattgtggg
    36361 agggggcatt ataagtgctg actcaggaat tgatatactg gatagcaaag gcaaggcttt
    36421 gacgagattt gggtcagagg gctgggtaag caagacaact gcttttgaat ttgcacaaat
    36481 gaatggctat aatgtaattg cctgcggagt taaccataga accaaccttc atttgtacag
    36541 agtagaatct tccaacccgg accaaaataa aatatcctat agaagaattg tttacaatga
    36601 acttgcaggt gctgttacag gtatagcatt gattccatat tatgaaagaa tatttgccgg
    36661 gacttcacag ggatttgtat cagcatatga tttccatgga aaagagttat ggatggagat
    36721 gatggaaggt gccgtaacag atattgccgc ttggaagaac agtataattg taactgagaa
    36781 aacagggaaa gttaatataa tgacactgga tggcaagctc gaagcttcaa caaaactgga
    36841 tactgctgct gcaaatattt tgaaaggttc caagaatcta tatttcattt ccggaaattg
    36901 catattaaaa atctagcagt acaagtaaaa agagaaaagt atatagttca ccttcgcaaa
    36961 tagtgtaaaa atattgaatg gcggtatatc tcataaataa cctggagact tgactccaaa
    37021 agcaaaatcg aaaatttgca tatgtatacc gccttctcaa tatctgcaga cattattgct
    37081 aatgcaggaa ttatatacat tcttacaaac acttgtaata ttcttcctca acctcatcaa
    37141 aggagaagct ataccctgca ttattaagta tatcaagggt tgtcttcata acaaggtcag
    37201 gtttaatatc ttcaacttcc agaaggaagg tcaaatattc aaatgtcata tcaaacagtt
    37261 ctgcccaatt gcaaatttct ttaaggttta ttgaatcttc cacagtttcc tccctcttat
    37321 ctttgtcatt ctataatagt attttagcat tttatttttt attatacaat tacgtaaaaa
    37381 tgttatataa taaaaatgta ccgcagtata tagctgcaaa taagtaagca tataaagggg
    37441 gggttttttt gaaagtaatc aaaaaagatg gaagagtgca gcccttcgat ttatcaaaaa
    37501 taatatttgc tcttgaacgc atatcagatg aatgcagtca accgtttacg caatcggatt
    37561 taacctttgt ggcgaagaat atagaaaaga gcattagagc ggctatggaa aacaaggatg
    37621 aagatataat ccacacatcc aagataagaa ctattgttat aaaagaattg gagcaggcag
    37681 ggtttaaaca tattgcagat tgttataaag aatatgtcaa aggttttgga aaacccgatt
    37741 ccccaaataa tatataacta atatatataa ctaatatata actgtaagca ataaactaag
    37801 acaacagccg gaagcaatag agttgagaaa tttgctgagt catcaggagc agtttccaat
    37861 gactcagcaa atacaactat tccttctcaa acatatcttt tccaaccccg caaattgggc
    37921 atacccaatc ctccggaata tcctcaaatg cagttccagg agcaatgccg ccgtccgggt
    37981 ctccttctgc agggtcatat acataaccgc aagctgtaca tacgtattta tccatatttc
    38041 cactccttta gataagttga attctttttg gcaagttata taatttaatt taactgtacc
    38101 tgctaaataa tctacccgtt ttcctatcat agtaacagtt atttcactga taagaggtat
    38161 aaattttaaa ttgtatgcag aaaatgtcct caaagaaatt aaaacaaaaa cgggtccttt
    38221 tgaattctcc ataatacatg ctttattccg ccggctatca ggtcagccat cttcataacc
    38281 aaaaataaac gggtattttg aaggatcagg aaatccataa aacctcccag gttgacaata
    38341 cctactatgt gaatattacc tataggggga agttcttttt ttaaagccga tccagggtta
    38401 atagggcctt ctcctataga tacaaaacct acatgctcca ttttgcctaa gcaggcatct
    38461 atggcaatta taaagggatt tatacaattt atgtttatgt cttccattac tttacttaga
    38521 tttttggcat gaacagggtt ttcaagagta ccgtaaacag atatattttt tctattattc
    38581 atattcataa ttttatgtcc aataagcggg ccaagactgt ccccggttga cctgtcggta
    38641 ccgatgcata caaaaactat agatttatac ccatcggata tatctttaca tatcagctta
    38701 tatagtacat ctgcaaaata gtggaaagct cccggactgt ttatatctat gaaatgtttc
    38761 tctactgagg ttttaataaa catttattac cctccatact caaattatgt aattctatga
    38821 acaatgatag cttaccggta taaacaggtg gttttacccc agcaccttaa aataaagctg
    38881 taccatagta tataattggc gcatagttta tattattacc gttaccaata caaatattac
    38941 agaagtttca atattttcct tcaaatatag aaaatgctcc tcagtatatc ttttttgttg
    39001 atataaacga agtattttgt tattataaaa acataaataa taagtgggta ttttttaaga
    39061 ttaaggtaat gtagagggga agaatgataa gtgtatcctg aattagcata tgatacaaat
    39121 gatacaagag aaaaaaacag tgaaacgagc atttacaata ctgatggata tctagtcgaa
    39181 ggggagatgc ttccaataga agatgtaatt aacggaatgt tggactgggt aagagtgctt
    39241 gaccttaacg ggaatattat ctatataaat aaagctatgg aagaagctct taaacgtcct
    39301 gtagtaggta aaagatgcta tgaagctttt ggcagggctg aaccttgtaa agattgtgta
    39361 tctcataaat gtgcatttga tggtaaaact catgaaaaag aagaagtttt tgatgacagg
    39421 tacttctcag ttatgagttc accaataaaa aataaacatg gcgatgttgt tgctattgta
    39481 gaggttctga gggatgttac aaaggtaaaa cttctccaaa aacagataat ggaacagaac
    39541 aggaagcata gggatgatat tgaaatggca aaaaaaattc aattgagttt attgcctgtg
    39601 aaactaccta aagacaaagc cgaattttcc tatatctata ctccctgtga gaccttggga
    39661 ggagattttt tagatatttt tatgatagat gatgatcatt ttggactata cattgctgat
    39721 gtagccgggc atggaattac atcatctctt ttaactgtgt ttttaaaatc tacatttaac
    39781 aaggaaacct tgtctccttc acttgcttta atggaattgt acagggaata taataaaatg
    39841 ggatttgaca ctgatcttta tattactgtt ttttactcaa taattgatct caaaaacaat
    39901 actatcactt attcaaatgc agggcataat atttgtccta ttttatttag cagtaccaga
    39961 ttcgaaattt tgaggaacgc gggattacca atatgtgatt ggtcagagaa acctgattat
    40021 attgataaat ctgaactctt atttaaaaaa gacagggtat ttttctgtac agatggaata
    40081 attgaggtaa ggaacaattt aaatgagcag tttggtgagg aacgacttct ttataatctg
    40141 gcagcatgca aattttgtcc ggatgttgtc ctaaaaagca ttatagaaca agcttgcaaa
    40201 tttgcgggtg tagatgaaat atccactata ggtgatgata ttactatggc actgcttgaa
    40261 atagtttaat ggaggtgtaa atatgcagaa atatactgaa gaagacatat tgagaatggt
    40321 taaagagcag gatgttaagt ttataaggtt gcagtttact gatatattcg gaatattgaa
    40381 aaatgtggct atacctgcaa gccaacttga aaaagctcta aataataaat gtatgtttga
    40441 tggatcatct attgaagggt ttgtaagaat tgaagaatcg gacatgtatt tatggcctga
    40501 tttgaataca tttgttattt tcccgtggaa gcctcagact ggtaaagtag caagattgat
    40561 atgcgatgta cataatcctg atggaatgcc ttttgagggt gatccaaggt atgtattgaa
    40621 aaaagtcttg aagaaggcag aagatatggg ctttgatact ctcaatgtag gacccgagtg
    40681 tgaatttttc cttttcctaa ctggcagtga gggcaatccg actgtcaaaa ctcaggataa
    40741 tgcaggatac tttgatttgg gacctgttga tctgggagaa agcgccagaa gggatattgt
    40801 tgttgcattg gaggaaatgg ggtttgatgt agaagcctcc catcatgaag gcgctcccgg
    40861 acagcatgaa atagatttca aatatgctga tgctttaaca gcagctgatt caatactgac
    40921 ttttaagctg gtagttaaaa caattgctca aaagcatgga ctgcatgcat cttttatgcc
    40981 aaaacccata tatggaatta atggttctgg tatgcatacc aacatttccc ttttcacaag
    41041 cggggtaaac gcgttttatg atgaaaaaga tcaggttaaa ttaagtaagg aagcttattg
    41101 gttcattgga gggttaatga agcatgccag agctattgct gtacttacaa acccgattgt
    41161 taattcatac aagagacttg tacctggcta tgaagctccg gtgtatattg cctggtctgc
    41221 aagaaacaga agtccattga ttcgaatacc ggcagcaaga ggagccaata ccagggtaga
    41281 attaaggtgt cctgatcctt cttgcaatcc ctaccttgcc cttgctggta tattgtcagc
    41341 cggactggat ggaatagaaa acaggatatg cccgtccaac cctacagata agaatatttt
    41401 ctgcatgagc gatgaacaga tgcagaagga agggatattt tctcttcctg gaaatcttaa
    41461 ggaagcagta gacgaaatgg caaaaagcag tttactgaag aaagttttag gtaaccatat
    41521 atttgaaaag tatattgaag caaaaaatga agaatgggaa gattaccgca agcaggttac
    41581 accatgggaa ttaaaacact atttaaccaa atactaagta gaaagcagaa agtagaaagt
    41641 ggaaagtgga aagtgagggg agctatatta tgcctgcaaa acgcccttta attgggataa
    41701 ctccaggata tagttatgag gaacaaaaga tctatataaa caatggctat atagatgctg
    41761 tgaatcaggc aggaggcttg gccatacttt tacccctaac agatgatgag gaattagttt
    41821 cagtgttggt tgatacctgt gatgcatt
//
LOCUS       DAOSDD010000006        40473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_542_length_40473_cov_5.288510, whole genome shotgun sequence.
ACCESSION   DAOSDD010000006 DAOSDD010000000
VERSION     DAOSDD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 40473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40473
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_542_length_40473_cov_5.288510"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK733_01015"
     CDS             <1..211
                     /locus_tag="PK733_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ09160.1"
                     /translation="KKCPTGTCSKLRTYFINKEMCKGCSKCTRVCPANAITGKIKEPF
                     AINSSRCIKCGACVETCPFGAIATN"
     gene            379..2160
                     /locus_tag="PK733_01020"
     CDS             379..2160
                     /locus_tag="PK733_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOJ09161.1"
                     /translation="MKGTMTIDGQKIEFDNEKNILEVIRKAGIELPTFCYHSELSIYG
                     ACRMCMVQDKKGKTFASCSELPRDGLVIYTNTPSLQKHRRMILELILSNHDRDCTTCE
                     KNGKCKLQELAARFGIKKVRFGSRQNSLPIDESSPSIIRNPNKCILCGDCVRMCEEVQ
                     GVGVLGFAYRGAKIEVTPAFNRPLSHVDCTGCGQCALVCPTGALLVKNDTEKAWGALH
                     DKTKKVFAIIAPAVRVALGEEFGLSSGQIVTGKIVAALKKIGFDFVYDLGLGADLTVM
                     EESREFLNRLSKGENLPLFTSCCPAWVKYAEQQHPELLENISSCRSPQQMFGALIKEH
                     FNYENISCSTNCTSNFTSDFTSDCACDCACDDNNTVVISIVPCTAKKAEAIRPEFESI
                     GIKDIDAVITTQELAHMIKESGILFNELEPEAFDMPFGLSSGAGILFGATGGVTEAVL
                     RRCCSVKTPVNLKDIEFSGVRGMEGVKEASVRINGKTVGVAIVNGLKNAEFLIEEIQR
                     GNKQYDFVEVMACPGGCIGGAGQPMPSHFLTNNDRAKGIYSVDRTSQIKRPEENPMIM
                     ALYNGVLKNKSSILHVPKREADVDICS"
     gene            2199..2447
                     /locus_tag="PK733_01025"
     CDS             2199..2447
                     /locus_tag="PK733_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ09162.1"
                     /translation="MLTISICVGSACHLKGAYNVINGIQKLIESNNLGDQVTVKAAFC
                     LGECTKAVSVKINDDAVVSVNENNLDDFFHRYVQAKLK"
     gene            complement(2536..3078)
                     /locus_tag="PK733_01030"
     CDS             complement(2536..3078)
                     /locus_tag="PK733_01030"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ09163.1"
                     /translation="MRTLLEGENIKLTAIKEYDIPIIEDWFNNVRFLRYYDMLPAVPK
                     SQKQVVDMLKSYEDSQIRYIYAIRTNDNSRIIGVAGFDEIIWSNGVATIFIGIGNNKF
                     TGKGFGKEAMRLLLDFGFNELNFYRIQLNVISYNEHAIKLYESLGFIREGIYRDFIYR
                     DCKRYDMYLYGLLRQEWGSK"
     gene            complement(3195..4094)
                     /locus_tag="PK733_01035"
     CDS             complement(3195..4094)
                     /locus_tag="PK733_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOJ09164.1"
                     /translation="MEFKKMDIEHRNIFQKYIGKYDFCTYEYSFLTLYIWRKMLNTEF
                     AVIDDALIIKKYCKKVGTYFMQPIGYKKEDLVGIVHKLNKIRENSADFKSLFRDVETS
                     FLYELLDMFEGNICLCEDINNFDYIYSSKDLISLSGKKFHRKKNQYNQFISSYDYKIK
                     DLLEDGVIKDCIDFAHSWYENRKGEDEQLMYELDSIRDILPKYQILGIKGMAVYVSGK
                     MAGFTIGEKVNSNMAVIHIEKGDLEYDGIYAFINKTFAENYFSDVKYINRQEDLGIEG
                     LRKAKRGYNPVKLEKKFAVDLYS"
     gene            4482..4739
                     /locus_tag="PK733_01040"
     CDS             4482..4739
                     /locus_tag="PK733_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ09165.1"
                     /translation="MLRIYICVGSSCHLKGSYCVINTFEELIKRYGLEDLVELKASFC
                     LGRCTKGVSAKVGDTFIEDLLPENAEERFNEFILQRLMVRV"
     gene            4736..6571
                     /locus_tag="PK733_01045"
     CDS             4736..6571
                     /locus_tag="PK733_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOJ09166.1"
                     /translation="MNVIQFKEANCKNCYKCIRNCPVKAITFINDQAKIIDDECVLCG
                     NCLIVCPQNAKTLKCDIDRVKNYISRGDKVYASIAPSFIAAFEDISREKMYTALKNLG
                     FTYMEETAIGASAVSREYQALIGQGNMKNIITSSCPTINFLIEKHYPELLDQLAPIVS
                     PMIAHGKMLKAVYGSRIKVVFIGPCISKKEECNDLQNDGVIDAVLTFEELEKWMQQEG
                     LTFEDSIIASGDIIRNTVSRFYPTPGGILKTLNSRGLRNYAFVSIDGIDSCIEILDTL
                     KKGELSNYFMEMSSCKGSCLAGPYIKNTRGGILEARNRLSNYISGNLNIRNSVPANAG
                     LSKNSSTPVLDNKNKKTDMAENNTSNLKIALTEDVKVNFHKKYIDRSKKYLIPDEATI
                     RNILSKIRKFNKDMELNCGACGYPTCRDKAIAVYNNKANLEMCLPYMREKAESISNIV
                     INATPNAIIALDEDLTIKEINSGAQKLLELKNGNYIGRNVYEILDCPDFEYVRESGQN
                     LLDEKYYYEQYKITVEQSILSIPDHHLLIVILKDITADEKRKQQIYKVRAETIDMAQK
                     VIEKQMRVAQEIASLLGETTAETKVTLTKMKKTIQTDMGDTDNIS"
     gene            6668..7840
                     /locus_tag="PK733_01050"
     CDS             6668..7840
                     /locus_tag="PK733_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ09167.1"
                     /translation="MRLYVDVSYESLNKYNEELCGDTVEVIRNGNSVTVVLADGLGSG
                     VKANILSTLTSKIIGTMLVNGASIDEAVETIANTLPVCKEREIAYSTFSILQVFNSGE
                     CYLVEFDNPSVIYLEGGKVKYLESISREINGKHVKESRLQLSEDDTFVLISDGVVHAG
                     IGSTLNLGWQWENVAEYVERTWKKDLSAKKIAKLLVSVCDNLYAQKPGDDATVAAVKI
                     RSPLKVNIMVGPPVDRNQDNYVVSRFLSQEGMKVVCGGTTSKIVSRETGREIVTSFNY
                     FNPAVPPIAEIDGIDLTTEGVLTLGKTLDYVRRCLSTESTIHDLLHLKDRDGASKLAN
                     ILLEDGTSVNFFVGRAMNPAHQNPDMPLNLGLKLKLVEELAAYLKSIGKEVSIEYF"
     gene            7973..9610
                     /locus_tag="PK733_01055"
     CDS             7973..9610
                     /locus_tag="PK733_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ09168.1"
                     /translation="MVIRTITLKLYKPSRRKREIIDDALLRYSMAYQYMLDKAFEQIE
                     EIREKYKEPYGIYSSFKLSKWMDSTLSEELNEFGVQPFKDSLKIDLGMSLAGHLNLKR
                     IRENTSFPLVYLPDDRYDSSYDALMDEIISEGLNDNLKRHKCEKELLRLERKSASLSP
                     IFFCRCATNRDYCLLYDVINKKYYAKLYLMNRSDDRKKALNPSENMLLRYIHKDGGML
                     KPSDRKERYIIVPLSFGKWQEQYLKTALVDPSIIKTARLIKKKDEYYLAVNIDIEEEK
                     AVETETYLGLSRDLKALACYTIVDSGGQVLETNKFTNLEVNKFAKMKCISSGAFPADV
                     LHSIANEIVALSANNKSQVVVQSMIDKSDRLVWRDEQGRVFKPLLDCRRYNDLVKILE
                     YKLPSRGLPPPIKVSPVGIFYKCPACELQAKKNRISKDMFICTACGQVMEIGHLGSLN
                     LAKKPIQYSKDVLKVNVESTPIGVRLINKELGLDFKPSEDINWFDDFKKEIVDIIENF
                     NANMPGVKDDSKSFKMKYSIIKRLESGKDLSEMIRLS"
     gene            complement(9893..10405)
                     /gene="dut"
                     /locus_tag="PK733_01060"
     CDS             complement(9893..10405)
                     /gene="dut"
                     /locus_tag="PK733_01060"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOJ09169.1"
                     /translation="MDIEVFIELCREGAVLPEYAKPGDAGMDVFAAEDVMIKPGETAI
                     IPTGIKLAIPEGFEIQVRPRSGISYKTPLRIANSPGTIDSGYRDELGIIMSNSSVAEF
                     NTACCVNFSNEILTLESKGNKMGTYKIRKGDRIAQIVLQELPRIKFTVVESVKDIGTN
                     RGGGFGSTGV"
     gene            complement(10465..13377)
                     /locus_tag="PK733_01065"
     CDS             complement(10465..13377)
                     /locus_tag="PK733_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOJ09170.1"
                     /translation="MKDKIITKKRLPELLAPAGSMEALKAAVENGADAVYLGGKGFNA
                     RQYAGNFDNIELEKAINYSHLRDVKVYLTMNTLVSDAEMKDALNLVGQACEMGIDAVI
                     IQDIGLAGILKEKFPNLDLHASTQMTVYNPEGVVFLSKMGFKRVVLARELSIEEISYI
                     VERCNTEIEIFIHGALCISYSGQCLMSSMLGGRSGNRGKCAQPCRLPYQLVMADKKRC
                     AEDKKRYDLEEYVPSNKNRLYLLSPKDICTVDNLEEIISTGVKSLKIEGRMKSPEYVA
                     TVVRIYRKYLDAAVAESKEHVSKEDMRDLHQVFNRGGFSKGYLFGKCGADMMCMEKPK
                     NWGIYIGKVISYDSKSKLARIGLDEPLSIGDGIEVWNNEDGYGKFENRRSGNRKEADK
                     SGYNRIDLESPGTVVSEIKKNDINVKEANAGDVIDVGRLTGIISKGDKVYRTSEKKLM
                     EAARETWLHSSGSSGRKVHITGELNIKAGNRPVFSVKDNHENRVELVGECIAEKAMNL
                     PLTEDRVRDQISKTGGTPFEFDNLDIRIDEGVSLPVKELNKLRRQALEKLEISRIMQY
                     RGNQYRKGKSLSSAINLSIEILSSEVVSADDVELCKPKISVLFYEFDLQKDRYYSELN
                     ADRIYLPLSFFLHFKESSKVKAETKAEDKAKDETSLVKTVLKSIKNRFSEVFVWLPSI
                     TRGNYDSLIKSNLPWIVESGIDGVLAGNWGTLECVLKIAKLNPGPCTSANLKVACDYS
                     FNAFNSISVRNLYNMGADGVTFSYELTLEQIKNICNKGNNEGLNLQKEVVIYGRIPLM
                     TSEYCPVGATIGGVLTESKCKGSCTQGTYSLLDRMNEKFPMFCDRIDCRSTIFNSKIL
                     FLADNIDKIANSGADIFRINIWDESPDEVVNLVNMHRELINSKIQLKNDFVADDEHFD
                     IRCEDKLGDKPDNRLDNRLDNKFDNRLEVKLEDKYEREIARIKDKGFTRGHYFRGV"
     gene            complement(13432..13866)
                     /gene="zapA"
                     /locus_tag="PK733_01070"
     CDS             complement(13432..13866)
                     /gene="zapA"
                     /locus_tag="PK733_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOJ09171.1"
                     /translation="MTAKNRVLLRIAGMEYMVRGTESEEYMHKLGLFVDKKMGEIIKS
                     NNKLSTCMAAVLTALNIADDYTKTYEKSEKLENELKKLREELSVLKAENENLAKEYSN
                     VNGLYKQLQLDFVKSDTELKEVRKSMEKLEGQNNKRLILTEE"
     gene            complement(14112..15512)
                     /locus_tag="PK733_01075"
     CDS             complement(14112..15512)
                     /locus_tag="PK733_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09172.1"
                     /translation="MESMRFKRFVWMLNEEDKGFALKDVPGGYVRIEVRESKGKFYAM
                     VQNLKENRGKDKYSIYIMRHRNSEIYPVKVGNIQVNDGRGLLLWDFDPNYVGGSIYPI
                     TDFNMAAVILDGDNYNAGTIQCPLAAYSAGKVKWKEAFENYVKRKKEAEVAADHSKKE
                     KSIIIEKQKTDESGSTSEREEHPLKEEYEINRNYAEEHTIDEILSIVDDANTDNKHEV
                     YSVDDIYSKYTGKVESKYSFTENIPGIDELTCGKIYPGCISEFNSNSNNREETAQPDT
                     EQPVPDETERTAQPDAEQPVPDETERTAQPDMEQPVQPDIEQTVQDNNIYERKKNKPD
                     INKLVQCFDKEFKRYDPFNVRRRDYKWWKVTSPVNLINLLEQCDINVPRIFNPSIIMS
                     YFKYRYLIAGIFSSRKRGRDYFVCGIPGTHNIDDSPFGNLCRWVQVERNRSKYGAFGY
                     WVAYIDPHTGELLRFS"
     gene            complement(15595..16455)
                     /gene="rsmA"
                     /locus_tag="PK733_01080"
     CDS             complement(15595..16455)
                     /gene="rsmA"
                     /locus_tag="PK733_01080"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ09173.1"
                     /translation="MGAGTKETINKYYIKPTKSLGQNFLVDDFACRGIIEALNIKNDD
                     LIIEIGAGLGALSKDIAEKAGRFIAVEIDKHLIPALTDLLKDYSNTQIINTDFLDLDI
                     EKEIIMNGFKPNSIKVVGNLPYYITTPIIMKLLTIENAIDIIVIMIQEEVVDRILAKP
                     GTKDYGALSVAAQYFSTPEVLFNVPPHCFFPKPKVSSSVVRLIRHKVAPVYIKDKKLF
                     FNVVKASFGQRRKTLLNSLFNSNMFSLNKEEIKAVLKSQGIDENARGETLSIVQFAQI
                     SNMLSQKTLS"
     gene            complement(16495..17619)
                     /locus_tag="PK733_01085"
     CDS             complement(16495..17619)
                     /locus_tag="PK733_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOJ09174.1"
                     /translation="MHFIKKSFFDKVIFGKNLIGRGIREIIDEMGIKLLFKVVSIIIA
                     VSVVTAFAGAEVLKAPVKTVSINDDGSIVILKTKGETIEEALQQNGINVKKEDYVYPS
                     GEQKLISWKNNEILIKRATPVSIYVDGKEEQILTQMNTVKGVLEANQIAFSESDLLEG
                     ANIEDTVFPGMKFRIIRIKEDLITEKEAISFKVEKRPSQRMEQGVEKTVRDGKEGVKE
                     YLYRVVYEDGEIKTKELVKESIAMVPIDKVVEYGTIATGTTSRGEKFRYKKVIDARAT
                     AYTASFKDTGKNPDHPYFGITYTGVKVKKGIIAVDPKVIPLGSRVYIEGIGKTPDYGY
                     ALAADIGGGVKGNMVDLYMDTQKEADNWGIRKVKVYILAD"
     gene            complement(17859..18611)
                     /locus_tag="PK733_01090"
     CDS             complement(17859..18611)
                     /locus_tag="PK733_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09175.1"
                     /translation="MKKFIVIMSFVLIIIIFVGLNYLLWERENREKDIRGLQEASTSG
                     NLTINALNRQLENLENTLKLRNDNIDKITNENNALRKDIEELKQENIRLNNMIRHKAE
                     VINSVYKDIQQGGPIETSIKKWAEYISSANYKEAYSIWYGNGKEAEESLDQFTNKYKN
                     IVNSIKIKSITPYNIDTNAGKNMDKDNIDRKIIDEYDKGDVFLLVELDVKLAEWAVKY
                     DLLFNQGENKKIIVLSYNAKDKKWYISDIRNP"
     gene            complement(18655..19422)
                     /locus_tag="PK733_01095"
     CDS             complement(18655..19422)
                     /locus_tag="PK733_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ09176.1"
                     /translation="MLFDTHAHYDDERFDEDRYELLEKIHREGVSYILNAASDIDSAK
                     MSIMLAEKYDFVYAAAGVHPHNAGKTDDSTMASLKSLISCNKVVAIGEIGLDYYYDFT
                     PADTQKKWFAEQIRLAKRVKLPIIIHNRDSHEDVMRIIKEENARDAGGVFHCYSGSVE
                     MARDVLNNNFYISFGGPVTFKNSKKVLDVLRFVPEDRILVETDCPYLTPEPFRGKRNH
                     SGYLEYIVKKIAEIKGSTFEKMAELTMENGKRLFNIK"
     gene            complement(19541..21547)
                     /gene="metG"
                     /locus_tag="PK733_01100"
     CDS             complement(19541..21547)
                     /gene="metG"
                     /locus_tag="PK733_01100"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ09177.1"
                     /translation="MQKKKFYITTPIYYPSDKLHIGHSYTTVAADTIARYKRLKGYDV
                     MFLTGTDEHGQKIQRKAQEKGVTPKEYVDYIVSGIKELWSAMKITNDRFIRTTDKYHE
                     EAVQKIFKKLYDQGDIYKSEYEGWYCTPCESFWTETQLVDGKCPDCGRTVELTKEESY
                     FFKLSKYQDRLIKHIEENPEFIQPVSRQNEMINNFLKPGLEDLCVSRTTFNWGVPVTF
                     DEKHVVYVWIDALSNYITALGYLSDNDSDYRKYWPADVHLVGKEIVRFHTIIWPAMLM
                     ALGEPLPKQVFGHGWLLLEGGKMSKSKGNVVDPVVLIKKYGLDAIRYFLLREVPFGSD
                     GVFSNEALINRINSDLANDLGNLVSRTIAMIEKYFDGVIPAERTAGDFDNDLKNVIAQ
                     TPGSVEELLDKLQFSTALTEIWKAISRTNKYIDETMPWVLAKDTSNKGRLAAVLYNLA
                     ESIRIISVLVQPFMPETPEKIWHQLGIAGKEELVSWESAQNWGMYPDGIHVNKGDVIF
                     PRIDLKKELENLEIMENANSGTQLKNKDCGKNKSDGKDENTKNNDKIKEPESNSITIE
                     DFTKVELKVAKVAEAEKVEGSDKLLKLKLEVGNETRQVVSGIAKYYSPEDMKGKYVIL
                     AANLKPVRIRGIESQGMILAASNDKKDLVLVTVDGEIETGSRIS"
     gene            22074..23294
                     /locus_tag="PK733_01105"
     CDS             22074..23294
                     /locus_tag="PK733_01105"
                     /inference="COORDINATES: protein motif:HMM:NF022233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2600 family protein"
                     /protein_id="HOJ09178.1"
                     /translation="MDEIMYSAGNLLINLVFKSIPEIRKQMKAQRKIIEELFNTFTFI
                     DNITGYNNSTDSKPYSVSLAGSHLDPYTESCTELYTKQYIEPHVELYTVPEKHFFVNK
                     EYVQALYSLFPNVNSKKVINFMISFNMLTMYLDQLCHKEQIKDESTVRQFYLSMLDAV
                     DPDREISNYCKYFPSKSENNYINQFVECCRNELEGIPSYKLVKENIKKYVYFYSDFHT
                     YKHISYGENRDDYLFTWSGYYLGQYADIYPWEFQAAACSCLGIFALLSSAFDPSLTDE
                     MISSIDKAYFPWINGLQVLLANYLCLYHDIINSNLNFTNYYKNLKLCEERIIFFFKQS
                     LKACSQGNLSDNHLSVVNLILGIYLSDPRADLGLKKITSKNLIKSGDILYHRLLKLIM
                     FLSLPPKYQCAYPY"
     gene            complement(23259..23792)
                     /locus_tag="PK733_01110"
     CDS             complement(23259..23792)
                     /locus_tag="PK733_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOJ09179.1"
                     /translation="MRIIKVIADFAIPVTFLIILIIGLYRDIKVFDVFVEGAKDGIYT
                     VLRIIPSLVGLFAAIGVFRASGAMDLLVFSVKPLADLFNIPSEVLPLVLLRPISGSAS
                     LALVSDIIKVHGPDSFIGRLISTMMGSTETIFYTLTVYCGAAGIKNIKHTLLAALIAD
                     AAGILASVWICTLIFGR"
     gene            complement(23789..24409)
                     /locus_tag="PK733_01115"
     CDS             complement(23789..24409)
                     /locus_tag="PK733_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOJ09180.1"
                     /translation="MLNYIWFAMLVFGFIIGIMNGRIAEVTNAVIESSGDAIKLSINL
                     LGIICLWSGLMKIAEKSGLVRNLASLVRPLIHFLFPDVPKNHPAAWTIIMNMSANILG
                     LGNAATPLGLKAMEELQKLNPAKHSVSNDISMFLVLNTASLQLIPTSVIAIRAQAGSA
                     NPAEIISSVWIASVCAATCGVISVKLLSRIWGKGRKVKKARKGAGR"
     gene            24785..25024
                     /locus_tag="PK733_01120"
     CDS             24785..25024
                     /locus_tag="PK733_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ09181.1"
                     /translation="MKSTGIVRKVDELGRVVLPIELRRTLDIAEKDALEIYVDGSTII
                     LKKYEPACIFCNDAKDVVSYKGKNICQNCMKELKK"
     gene            complement(25099..25989)
                     /gene="rsmI"
                     /locus_tag="PK733_01125"
     CDS             complement(25099..25989)
                     /gene="rsmI"
                     /locus_tag="PK733_01125"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ09182.1"
                     /translation="MDEKAKLDEKAKPDEKGTLYLVATPIGNLEDITLRALRILKEVD
                     LIAAEDTRQTIKLLNHYDIKKSLTSYYEHNKATKGNYLLNLLLEGKNIALVSDAGSPG
                     ISDPGEDIVRLAIENGIRVTMVPGPVAAVTGLVLSGLPAGRFVFEGFLPMNKRSRKER
                     IMFLKNEPRTIILYEAPHKLIYTLKDLYNILGERQIVLARELTKRFEELIRCTLSEAI
                     SKYEEESPKGEFVIILEGVDDSSAVNEERRLWEDTDIKKHMEIYLHQGLSRKDAMKKV
                     AEDRGVSRREVYQSLIEEKT"
     gene            complement(26072..26827)
                     /locus_tag="PK733_01130"
     CDS             complement(26072..26827)
                     /locus_tag="PK733_01130"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOJ09183.1"
                     /translation="MRDNPEVFLKENETIDDLQYKGLMLIQKEHGFRFGVDAVLLANF
                     AEIRKGDLVLDIGTGTGIIPILIAGKTEAGKITGLEIQEEMAEMAERSVKLNNLQDRI
                     TIVQGDIKNGLEYFGPSKFNLAVCNPPYMNKGGGIINNLDLKAVARHEITCTLEDIIK
                     ACSRLIVPGGRLAMVHRPERIVDVLYLMRLYSIEPKFIRFVHPSPNKKPNLFLVQGTR
                     GGNAGLKVMDPLYVYNAEGNYSDEINRIYHRIE"
     gene            complement(26964..27134)
                     /locus_tag="PK733_01135"
     CDS             complement(26964..27134)
                     /locus_tag="PK733_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ09184.1"
                     /translation="MSYYITDECISCGACESECPVSCISEGDGKYVIDEDACIECGSC
                     AGVCPVDAPQQK"
     gene            complement(27241..28113)
                     /locus_tag="PK733_01140"
     CDS             complement(27241..28113)
                     /locus_tag="PK733_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOJ09185.1"
                     /translation="MVKVVGVRFRRAGKIYYFDPEDLDIPENVGVIVETARGVELGHV
                     VLANREVPENEIVAPLKKVIRIATEEDIKHHNENIAREKEALEICLKKIKEHNLDMKL
                     INVEYTFDNNKILFYFTADGRIDFRELVKDLAAIFKTRIELRQIGVRDETKMMGGVGI
                     CGRVLCCKGFLCEFQPVSIKMAKEQGLSLNPAKISGCCGRLMCCLKYEHEAYEEILKR
                     APKAGAIVETPDGQGVVMEINLIKENVKVKLDKGNETDLQIFDLSEIRIIKDAAMPQD
                     DQDIDLEALKELED"
     gene            complement(28119..29159)
                     /locus_tag="PK733_01145"
     CDS             complement(28119..29159)
                     /locus_tag="PK733_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HOJ09186.1"
                     /translation="MDYNEIVGQKEIIGKLKKAVIDNKVGHAYIFSGPKGIGKRTVAR
                     AFAHMLLCLMPGENGPCGVCRPCILWNENSNPDFYEIETSDANISIDDIRSMQAKIVV
                     RPLYSDRKVYLITDADKMTIQAQNCLLKTLEEPPGYTVIILTTANYEALLGTVRSRGT
                     KYTFGKYSHNEVRAVLKSKFYQDSLKETIDDFGNSKDVVKDAEKMEFIVHYSDGVIGT
                     ALELAVSSEFASLREKAFEILLKLSSLDGMEYFDICDFFDDNKSNIDNLLDIMSIYYR
                     DLLMARVNGKENMLINSDKKSKIIEAARKYSVQTLVRNIEAIEFTRRSIKQNANYQLA
                     VETMIMKLREEI"
     gene            complement(29214..29666)
                     /locus_tag="PK733_01150"
     CDS             complement(29214..29666)
                     /locus_tag="PK733_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOJ09187.1"
                     /translation="MKIEETLNNRPNLTGMVISDVKKEVEADNKTFQGSLKQAEYKSY
                     EEKLNQLADRIFEQSKKLEKRMDIKEYIVYKRSISEFMNEFLNNSYKFSRQNMMDRRG
                     NYRTYSVVKKINSELDVLAKDLLENQQDKLKVLQRLDYIRGLILDMLM"
     gene            complement(29713..30042)
                     /locus_tag="PK733_01155"
     CDS             complement(29713..30042)
                     /locus_tag="PK733_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOJ09188.1"
                     /translation="MKLVFAIVHDEDGPKVMDELNRNGFSVTKLCSTGGFLRAGNTTL
                     LVGVTEEKLDTVINIIKQKSKSRKQAINSTMTPNNMGGMFMPYPVEVIVGGATIFVIN
                     VDRFEKA"
     gene            complement(30121..30753)
                     /gene="tmk"
                     /locus_tag="PK733_01160"
     CDS             complement(30121..30753)
                     /gene="tmk"
                     /locus_tag="PK733_01160"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ09189.1"
                     /translation="MERGLFISVEGIDGSGKTTQIGLMKSYLEEKGYEVIVTREPGGT
                     SIGEKIRSILLDPQNSEMDQVTEMLLYASARAQIIHELIKPAIDKGKIVICDRFLDSS
                     YAYQGYGRGIDIELITRVNSVAVCGIMPDITFLFDIVPETALKRRFASSTPDRIEREE
                     LDFYTRVYNGYKELAALYPERIKIISTDKSPEDVFSIVKRNLHHVLNSTL"
     gene            complement(30885..32369)
                     /locus_tag="PK733_01165"
     CDS             complement(30885..32369)
                     /locus_tag="PK733_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ09190.1"
                     /translation="MNHFKETRINAPIYEALKNYIGENPIPFHMPGHKMGKGIFPEYA
                     AIISQLDVTEIPGTDNLHFPIGAIKESQELAAYAFGAVKTYFLVNGSSCGIHAAIMSV
                     CRPGDKIIVSRDCHKSVIGGMMLAGANPVYVPVGLDDSFGIPLVVGTGELERTLEENP
                     NAAAVLITRPNYYGVCSDIERIAQITHSYRKILIVDEAHGAHFNFHSSLPASALSAGA
                     DICIQSAHKTLPAFTQGAYLHLGSHLIDVDRLEFNLRLLQTTSPSYIIMTSLDIARSI
                     METHGGELLERLIDNIEWFIDASNLISGIKVLQEKDLISWDGMKIEKDITRVVINVRE
                     LGMTGYETAKTLREKFHIQVEMADLHNIVCIATVADGRKEIEALYLALKQIAHEKDNK
                     VERSVGILLGTTTSATKNLPIPKQGMKLQDVSRSKGKYIALKNSMGEISRDFIVPYPP
                     GIPVVCPGEIISGDIVEYIYGVIEAGGSVNGIDTNDEVDVVDIV"
     gene            complement(32366..32953)
                     /locus_tag="PK733_01170"
     CDS             complement(32366..32953)
                     /locus_tag="PK733_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09191.1"
                     /translation="MAKNQEYTRERTGEYEDRQKNNKQQKYPGNQERRKDFIQQNRYH
                     GKESLNEITKGTQTENAREKTRENTKEILKERGKTFLKEKTMETPGNRNRIKEVSKER
                     NKEVHRNRMKEIPKERTKEGPGEKAKGMLGDISKEGFREKEKSREKPQAYASRNYNKR
                     IRTDETLDDIKEDIVRIEKEIRLEIKEIRSLKLGL"
     gene            complement(33028..34035)
                     /locus_tag="PK733_01175"
     CDS             complement(33028..34035)
                     /locus_tag="PK733_01175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOJ09192.1"
                     /translation="MKYKILEILKNNKGNFISGELLSSELGVTRTTIWKHIKDLKVEG
                     YNIESSSKKGYVLVDIPDILNAYEIGYNLHTECLGKEIYCLQSVDSTNIYSRKIAHEG
                     CKDGTVVVADLQTAGKGRLGRAWDSKDRKGIWMSVVLKPAILPSKVQVITLAASIAVV
                     SGIMQAAGISTGIKWPNDIILDGKKVCGILTEMSSELDRVNHIILGIGLNVNHDEEDF
                     PPELRGKATSLKIHKLSEGISGKWFRRSDIIKGILENLEESYNYIKDGRTDVLLEKWR
                     EFSITLGKEIRVVSENGEYRGIALGITEDGKLVVKGEDGATHQFISGEVSVRGMDCYV
                     E"
     gene            complement(34073..34729)
                     /locus_tag="PK733_01180"
     CDS             complement(34073..34729)
                     /locus_tag="PK733_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09193.1"
                     /translation="MAEVYYYVPAEESENIVECGLKLSVWHSKEIMIEGIIKKCISAL
                     LNPKDDINKFNDEAYKCIKLDLPPDYCYVADRYLYEAGVDMPEVMELYLKSIIPLEKY
                     IFGSYRLPECLVTTTVIPGQADLLNKAQDTPVLFNSSEELYLNNLIEFNKEKYYDFDD
                     ITMYFFYCKLSELKQIVKIEDKNRKIAVFIDNTDNGNERIYTIKIPETICNIENNSGK
                     "
     gene            complement(34770..35258)
                     /gene="folK"
                     /locus_tag="PK733_01185"
     CDS             complement(34770..35258)
                     /gene="folK"
                     /locus_tag="PK733_01185"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOJ09194.1"
                     /translation="MESEAYIALGSNMGNRVENLQRAINAISKLDKTSVVKMSRIYET
                     EPVGYKEQDKFLNMVIMTKTGLKPLELLDKLQGIENMLKRKRIIHWGPRTIDLDILIY
                     DNINIGLPRLTIPHPEMLGRAFVLIPLREIAPDMEFNHIGIDYFIEKCDDKEEVRIYN
                     NV"
     gene            complement(35371..35736)
                     /gene="folB"
                     /locus_tag="PK733_01190"
                     /pseudo
     CDS             complement(35371..35736)
                     /gene="folB"
                     /locus_tag="PK733_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
     gene            complement(35784..36605)
                     /gene="folP"
                     /locus_tag="PK733_01195"
                     /pseudo
     CDS             complement(35784..36605)
                     /gene="folP"
                     /locus_tag="PK733_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
     gene            36873..38252
                     /locus_tag="PK733_01200"
     CDS             36873..38252
                     /locus_tag="PK733_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09195.1"
                     /translation="MVDLFKLNTDELKALVIYKGVLEEGERFPKEFWTGEKEQVKGIK
                     IKCRVLTRYCFENLSRIEVADLSKYNLKQLKSLLVKYKLSGMVQRVFNHDVVAILKNA
                     YPEEFRTRKLKEWMWSKHGIWHDDNAIIEAIHDMVRKEGIRRIEDIPALNWKDRLLKH
                     GIYNVLAYFNWSIYALFNFVYPNKFHPTDFKYKTKWAAADSLDNAFHYMHKTFKKKKY
                     TLDEILLLNTSNFRGLGLAGMLASVFDSSTLRAKEYYLYKTIGDKAHIKELKEDIRKL
                     KKENYDKNIRKKLNEVSSVGGFIYNLHDNTTLYNYIKRHAKKNNMSINDFISSYGFTY
                     KSARKDIKNISKEDIWNLRKQGLTYVQIAQELESNPTTITEMCSRYFGGDPLIPRPIE
                     DYVTVQELMNKYRVDHKTVMRIVYENNFENHTTIRFRYLKKSEIEPALQEYVETSKHH
                     RFMVKRYAN"
     gene            complement(38344..38904)
                     /gene="folE"
                     /locus_tag="PK733_01205"
     CDS             complement(38344..38904)
                     /gene="folE"
                     /locus_tag="PK733_01205"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOJ09196.1"
                     /translation="MIDKERVKGAVREFLAAIGEDPDREGLKETPDRVARMCEEIFAG
                     VGQDPKNVIKIFQEDGHEEMVMVKDIPLYSVCEHHLLPFIGVAHVVYIPNKGKIVGLS
                     KLARIVDTIAKRPQLQERLTSEIADVIMESIKPFGVAVVVEAEHLCMTMRGVKKPGSK
                     TVTSALRGIVRTEAKTRAEVMALITR"
     gene            complement(39174..39449)
                     /locus_tag="PK733_01210"
     CDS             complement(39174..39449)
                     /locus_tag="PK733_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09197.1"
                     /translation="MDEFNNTENREEVAEARDSTLFLVIGWISAILSLLAYPFIFGVV
                     GAIMGILAVKNQSRAGLPLIVGSIVFMGIGMIFSGVIMNYVRHYLGM"
     gene            complement(39566..40069)
                     /gene="leuD"
                     /locus_tag="PK733_01215"
     CDS             complement(39566..40069)
                     /gene="leuD"
                     /locus_tag="PK733_01215"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOJ09198.1"
                     /translation="MIQGKAIKYGNNIDTDVIIPARYLNTHEPSELAKHCMEDLDKTF
                     ISHVEKGDVMVGGKNFGCGSSREHAPIAIKASGISCVIAETFARIFYRNAINIGLPIL
                     ECPEAAKAINKGDIVAVELSSGKIVNLTSGDEYKAAPFPEFMQEIINADGLIEYTKRK
                     ITLASNA"
     gene            complement(40110..>40473)
                     /locus_tag="PK733_01220"
     CDS             complement(40110..>40473)
                     /locus_tag="PK733_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ09199.1"
                     /translation="CTNGRLEDLRVAAEVMEGKKVHPDVRCIIIPATQKIWKQAMNEG
                     LFDIFVDAGAAVSTPTCGPCLGGYMGILAEGERAVSTTNRNFVGRMGHTGSEVYLASP
                     AIAAASAIAGRIASPDEL"
BASE COUNT        12105 a         8239 c         6276 g        13853 t
ORIGIN      
        1 taaaaaatgt cctacaggca catgcagcaa actaagaact tattttataa acaaagaaat
       61 gtgcaaaggt tgcagcaagt gcaccagagt ttgtcctgca aatgcaataa caggaaaaat
      121 caaagaacca tttgccataa acagctcacg atgtataaaa tgcggtgctt gtgtagagac
      181 ctgtcccttt ggggcaattg caaccaatta agaaacatgg ggagaaacat ggagacagtt
      241 ctcctgtttc ggggggtgtt tcagggggga cagttctcct gttttttcag gggggacagt
      301 tctcctgttt taacagaaag ggacagtcgt tgactagaga actgtcccca aatcaaacgg
      361 aaggagatta atcatattat gaaaggtaca atgactatag atggacaaaa aatagagttt
      421 gataatgaaa aaaatatact cgaggttatc cgtaaagcag gcatagagct tcctaccttc
      481 tgctatcatt cagaactaag catttatgga gcatgcagaa tgtgcatggt tcaggataaa
      541 aagggaaaaa cttttgcttc atgctcagag cttccgcggg acggtttggt aatatatact
      601 aacaccccat ccctccaaaa gcacaggcgt atgatccttg aacttatatt atccaatcac
      661 gacagggact gtacaacctg tgagaaaaac gggaagtgca agcttcagga gcttgcagcc
      721 aggtttggta taaagaaagt acgttttggc tccagacaaa attcccttcc tattgatgaa
      781 agcagcccat caataataag gaatcctaac aagtgtattc tttgcggcga ctgtgtaagg
      841 atgtgtgagg aagtacaggg agtgggtgta ttgggttttg catatagagg agcaaagatt
      901 gaagtaactc ctgcatttaa cagaccctta tctcatgtag attgcaccgg ttgcggccaa
      961 tgtgctttgg tttgccctac aggagctctt cttgtaaaaa atgatacgga aaaggcctgg
     1021 ggtgcccttc atgataaaac taaaaaggtc tttgcgatta tagcacctgc ggtaagagta
     1081 gctctgggag aggaatttgg tctgtcttca ggacagattg ttacaggtaa gattgttgca
     1141 gccttgaaaa agataggatt tgatttcgta tatgacctgg gactgggtgc tgaccttact
     1201 gttatggaag aatccagaga attcctaaat agattgagta aaggtgaaaa tctgccacta
     1261 ttcacatctt gttgcccggc ctgggttaaa tatgcagaac aacagcatcc tgaattgctg
     1321 gaaaatatat catcctgccg ctctccccaa cagatgtttg gagctttaat aaaagagcat
     1381 tttaattatg aaaatattag ttgttccacc aattgcacca gcaattttac cagtgatttt
     1441 accagtgatt gtgcctgtga ctgtgcctgt gatgataata atacagtggt catttctatc
     1501 gttccctgta ctgcaaaaaa agcagaggct ataagaccgg aatttgaatc aattggcatt
     1561 aaggatatag atgcagtaat aacaacccaa gagttggctc atatgataaa agaatcaggg
     1621 atactgttta atgagcttga gcctgaggct tttgatatgc cttttggcct ttccagtgga
     1681 gcaggtatat tatttggcgc gacaggagga gttacagaag cagtattaag aagatgctgc
     1741 agcgtaaaaa cacctgtcaa tcttaaagat atagagttct caggagtaag aggtatggaa
     1801 ggagtaaagg aggcttccgt tagaatcaac ggtaaaacag ttggtgttgc aatagtaaat
     1861 ggtttaaaaa atgcggaatt tttaattgag gaaattcaaa ggggtaataa gcaatatgat
     1921 tttgttgaag taatggcctg tcccggaggt tgtattggag gagcgggtca gccgatgcct
     1981 tcccattttc tgaccaataa tgacagagca aagggtatct atagcgtgga cagaacatct
     2041 cagattaaaa gacctgagga aaatccaatg ataatggctc tgtacaatgg cgtgcttaag
     2101 aataagtcca gtattcttca tgttccaaaa cgagaagctg atgtagatat ctgctcatga
     2161 aaaagcaact tgcccaaaaa aacagtgagg tgatttgaat gttaactatt agcatatgcg
     2221 taggcagtgc atgtcacctt aaaggtgcat ataatgtaat aaacgggatt caaaaactca
     2281 ttgaaagcaa taacctggga gatcaggtta cagttaaagc agctttttgt ctcggcgaat
     2341 gtacaaaggc tgtttctgtg aaaatcaatg atgatgctgt agtttcagta aatgagaata
     2401 atttagatga tttctttcac aggtatgttc aagcgaagtt aaagtaaatc tatactaatg
     2461 ggtgcatttt cttaatttaa agaaaatgca ccggctactg gttgtatttc acatatatga
     2521 ttcacgaatt gcagtttatt tgctgcccca ctcctgtctt aatagcccat ataagtacat
     2581 atcatacctt ttgcaatctc tatatatgaa atctctgtaa atgccttccc ttatgaatcc
     2641 aagactttca tacaacttta tggcatgttc attgtaggaa attacattca gctgtatcct
     2701 gtaaaagttc agctcattaa aaccaaaatc cagcaacagt ctcatagctt cttttccaaa
     2761 gcctttcccc gtgaatttat tattgccgat accaataaaa atcgtagcta ccccgttact
     2821 ccaaattatc tcgtcaaagc ctgcaacacc tattattctg ctgttatcat tggtccttat
     2881 agcatagata tatctaattt gtgaatcttc ataagatttt agcatatcaa ccacctgttt
     2941 ctggctttta ggcacagccg gcagcatgtc ataatatctg agaaatctca cattgttaaa
     3001 ccaatcctca attatgggta tatcatattc ttttattgcc gtaagtttaa tattttctcc
     3061 ttcaagcaat gttctcataa ataccctcca acctctaaaa agttaatcgt ataattacga
     3121 ctattggcac tttcccataa taactcagct atccaacaat agcagttaat caatatacaa
     3181 atcaactata taaatcaact gtacaaatca actgcaaatt tcttttcaag cttaaccgga
     3241 ttataacccc ttttcgcttt tcttagcccc tctatcccca aatcttcctg tctatttata
     3301 tacttaacat cactaaaata attctctgca aaggttttat taataaatgc gtaaatgcca
     3361 tcatattcta agtcaccttt ttcaatatga ataacagcca tattgctatt aactttttct
     3421 cctatagtaa aacctgccat cttgccgcta acatatacag ccatcccttt aatgcccaat
     3481 atctgatact taggtagaat atcccgtatg ctatctaatt catacataag ctgttcatct
     3541 tcgcctttcc tgttttcata ccaggaatga gcaaagtcta tacaatcctt tatgacacca
     3601 tcctcaagca aatctttgat tttataatca tagctgctta taaattgatt atattgattt
     3661 tttttacggt gaaatttttt accgcttaat gagatcaaat ctttactact ataaatataa
     3721 tcaaagttgt tgatatcctc acataagcaa atattccctt caaacatgtc caggagctca
     3781 tataaaaaag aggtttcaac atctctgaaa aggcttttaa aatctgcact attttctctg
     3841 atcttattaa gtttatgtac tattcctacc aagtcttcct ttttataacc tataggctgc
     3901 ataaaataag tgccaacctt tttacagtat ttttttatta ttaaagcatc gtctataaca
     3961 gcaaattctg tgtttaacat ctttctccat atgtataatg ttaagaaaga gtactcatac
     4021 gtacaaaaat catattttcc gatatatttt tgaaaaatat ttctatgctc aatatccatt
     4081 tttttaaatt ccattgcaag taccacctaa agtctgataa ttatttatgt caatattaat
     4141 gccaattcaa tattaatgtc aaccaaatat taaattacta tatcaatatt atctataatt
     4201 ttcatcttcc ttacaaatta taaagtaaca tataataaga ttatacatgc tttttatatt
     4261 tttttaaatg tagtgtacaa tattttttat tttctatatt tcgtatcatt tgtaccatag
     4321 gaagaaaatt cgccccctag ctaccgaaac ctattgacaa attattaaca aagtcgtaca
     4381 atataattgt taaatagtta acaatgcttg cagtcttatg ctgataactg caggtaatat
     4441 tatactaact ttgattaatc tttggtattg ggggaatggg tatgcttcgg atttacatat
     4501 gtgtgggaag ctcatgccat ttgaaaggat catactgcgt aatcaacacc tttgaagaac
     4561 ttattaagag gtacggcctt gaggatctgg ttgagttaaa ggcatccttc tgtcttggac
     4621 gctgtacaaa aggtgtatca gcaaaagtag gagatacttt catagaagat cttttacctg
     4681 aaaatgcaga agagcgtttt aatgaattta tactacaaag actgatggtg agggtatgaa
     4741 tgtcatacaa tttaaagaag caaactgtaa aaactgttat aaatgcataa ggaattgccc
     4801 tgtcaaagct ataaccttta ttaacgatca ggccaaaatc attgatgatg aatgtgtcct
     4861 ttgcggcaat tgcctgatag tatgcccaca aaatgcaaaa acattaaaat gtgatattga
     4921 cagagtaaaa aactatatat caagagggga taaagtatat gcatctattg ctccctcttt
     4981 tattgctgct tttgaggata ttagccgtga aaagatgtat actgccctta aaaatctagg
     5041 cttcacatat atggaagaaa cagcaatagg tgcgtcagca gtttcacgag aataccaggc
     5101 tctgatcggg caaggtaata tgaagaatat aatcacatca tcctgcccta caataaactt
     5161 tttaatagag aaacactacc ctgaactgct ggatcaactt gccccgatag tatcaccaat
     5221 gattgcccac gggaaaatgt tgaaagccgt gtacggttcc aggattaagg tagtttttat
     5281 aggtccatgc atatctaaaa aagaagaatg caatgacctt caaaatgatg gcgttataga
     5341 cgctgttctt acatttgaag aacttgaaaa atggatgcag caggaagggc tgacttttga
     5401 agacagtata atagcttccg gggatattat acgaaatacc gtatcccgtt tttatcctac
     5461 tccgggaggt attttaaaga cattgaactc aaggggatta agaaactacg cgtttgtaag
     5521 tatagatggt attgatagct gcatagaaat acttgatacc ttaaaaaaag gtgaacttag
     5581 caattatttt atggaaatga gcagttgtaa agggagctgt cttgcaggtc catatataaa
     5641 aaatactaga ggtggaatac tcgaagcacg taataggctt tccaactata tttccggcaa
     5701 tctcaacata aggaatagtg tgcctgcaaa tgcaggattg tcaaaaaata gcagtacgcc
     5761 tgtattagat aataagaata agaaaactga tatggcagaa aacaatacaa gcaatttgaa
     5821 gattgctctt actgaagatg taaaagtaaa ttttcataaa aaatacattg atcgctccaa
     5881 aaagtattta atacctgatg aagctaccat aagaaacatt ttatctaaaa taaggaaatt
     5941 caataaagat atggagctta attgtggagc ctgcggatat cctacatgca gagacaaagc
     6001 catagcagtg tataataata aagcaaatct tgagatgtgt cttccttaca tgcgggaaaa
     6061 ggcagaatca atttcaaata ttgttattaa tgccacacct aatgcaataa ttgcacttga
     6121 tgaggatctt acgataaagg aaataaacag tggagctcaa aagctgcttg aactgaaaaa
     6181 tgggaattat attggaagaa atgtatatga aattctggat tgtcctgatt ttgaatatgt
     6241 aagggaaagc ggccagaatt tactggatga aaagtactat tacgagcaat ataaaataac
     6301 tgttgaacag tcaatactaa gtatccccga ccatcatcta ttaattgtaa tattgaaaga
     6361 tataacagct gatgaaaagc ggaagcagca aatatacaaa gtacgtgctg agacaataga
     6421 tatggcgcaa aaagttattg aaaagcagat gagggttgct caggaaatcg caagcctgtt
     6481 gggtgaaaca actgcagaaa caaaagtgac tctcacaaaa atgaaaaaaa ccattcaaac
     6541 ggatatgggc gatacagata atataagctg aaatgtacga tatgagaaat acaagaaata
     6601 tgtaaaatat gtatggaaca tatgcagata atgcaaatat gcgaaatacc ggtattggtg
     6661 atgtataatg agactttatg ttgatgtaag ctatgaaagt ctaaataaat ataatgaaga
     6721 actatgcgga gatacagttg aagttatcag gaacggaaat tcagttactg tcgtccttgc
     6781 tgatggtctt ggcagtgggg ttaaggctaa tattctttca actcttacct caaaaattat
     6841 tggcactatg ctggtaaatg gtgcaagtat cgatgaggct gttgaaacaa ttgcaaatac
     6901 actgcctgta tgtaaggaaa gggaaattgc ttacagcacc ttttcaattt tgcaggtttt
     6961 taactcaggg gaatgttatc ttgttgaatt tgacaaccct tcagttattt accttgaggg
     7021 agggaaagta aagtacctgg aaagcatatc gagggaaatc aacggcaaac atgtcaagga
     7081 aagcagattg cagttaagtg aagatgatac ctttgtgctc ataagtgatg gagttgtaca
     7141 tgcaggtatt ggcagcacac ttaacctggg ttggcaatgg gaaaatgtag cggaatatgt
     7201 agaacgcaca tggaagaagg acctgtcagc aaaaaaaatt gcaaaactcc ttgtgtcggt
     7261 atgtgataac ctatatgcgc aaaaaccagg agatgatgca acagtagcag ctgttaaaat
     7321 acgcagcccc ttaaaggtta acataatggt tggtcctcct gtagacagaa atcaggataa
     7381 ttatgtggta agtagattct taagccagga aggcatgaag gtagtttgcg gcggcactac
     7441 ctctaaaata gtcagccgcg aaacaggcag agaaattgtt accagcttta attattttaa
     7501 tcctgctgta cctcctatag cagaaataga tggtatagac cttacaacag agggtgtcct
     7561 tacacttggc aagactctgg actatgtaag aagatgcctt tcaactgaaa gtacaataca
     7621 tgatctgctg catcttaaag acagagatgg tgcttccaaa ttggctaaca tactattgga
     7681 agatggcaca tcggtaaact tttttgtggg cagagccatg aatccagcac atcagaaccc
     7741 ggatatgcct ttaaatctcg gactgaaatt aaaactggta gaagaactgg cagcctacct
     7801 gaaaagcata ggaaaggaag taagtataga gtatttttaa gttttaagtg catggtatct
     7861 atttagtata tatttggcat ctatatagca tctgtatagc atatatatag catctttggc
     7921 aatccatatg atacctatta aaacttattt tggcacttgt ttaggtgatt ttatggtgat
     7981 tcgtacaata acattaaaac tttataaacc aagcagaaga aaaagagaaa ttatagatga
     8041 tgccctttta agatattcca tggcctatca atatatgctt gataaggctt ttgaacagat
     8101 tgaagaaatc agagaaaagt ataaagaacc ttatggaata tactcatcat ttaagctctc
     8161 aaaatggatg gacagcactc tgtcagaaga actaaatgaa tttggagtac aacctttcaa
     8221 agattcttta aaaatagatc tgggaatgtc ccttgccgga catctaaacc ttaaaaggat
     8281 cagggagaat acaagtttcc cattagtata cttgcctgat gatagatatg atagtagtta
     8341 tgatgcttta atggatgaaa tcatcagtga agggttgaat gataacttaa aaaggcataa
     8401 atgcgaaaaa gaattgttgc ggctagaaag gaaatctgcc tccttaagcc ccatattttt
     8461 ctgtcgttgt gccactaaca gagattattg tcttctttat gatgtaatta ataaaaaata
     8521 ctatgcaaaa ttatatctga tgaatcgcag tgatgacagg aaaaaagcat taaatccatc
     8581 agaaaatatg ttattaaggt atattcataa agatgggggt atgctgaaac caagtgacag
     8641 aaaagagcgg tacattattg tgcctttatc ctttggcaaa tggcaggaac agtatttaaa
     8701 aactgcattg gttgatcctt ctattataaa gaccgcaagg cttataaaaa agaaagacga
     8761 atattatcta gctgttaata tagatattga agaagagaaa gcggtagaaa cagagaccta
     8821 tctaggactg tcaagggatc ttaaggctct tgcctgctat actatagttg acagcggcgg
     8881 acaggttttg gaaactaata aatttacaaa tttggaagta aataaatttg caaaaatgaa
     8941 atgtatttcc tccggggctt ttcctgcaga tgttttgcac agcattgcca atgaaatagt
     9001 tgctttatcg gctaataata aatcacaggt agttgtccag tccatgatag ataaaagcga
     9061 caggctggtc tggcgcgatg agcagggcag ggttttcaag cctctgctgg attgcaggag
     9121 atataatgat ttagttaaaa ttctagagta taaactgcca tcaaggggcc ttccacctcc
     9181 tataaaggta agccctgtag gtatttttta taaatgtccc gcctgtgagc ttcaggcaaa
     9241 gaaaaacaga atttccaaag atatgttcat atgcacagct tgtggtcagg ttatggagat
     9301 agggcacctt ggcagtctta acctggcgaa aaaaccaatt caatatagca aggatgtgtt
     9361 gaaagtaaat gtggaaagca ctcccatagg tgtaagactt ataaataaag aattagggct
     9421 ggatttcaag ccttccgaag atataaattg gtttgacgat ttcaaaaagg agattgtaga
     9481 tattattgaa aatttcaacg caaacatgcc gggagtaaaa gacgatagta agagctttaa
     9541 aatgaaatac agtattatta agcggcttga aagtggaaag gatttatcag aaatgataag
     9601 gttaagctaa tgattaatgc tattatgcca gtttggaaat ataacaaagt agtattaagc
     9661 taatgattaa actaatactg attctgctgt aaaatccgaa ttgttgcagt aggatcagtt
     9721 aatatatttt ggtgcctttg ctgccgcacc aaggcctgaa agcaaggaat acacctgcct
     9781 ttggactgaa acctgcagag cgggaatgca tgttttactg cattaaagag cagctatcta
     9841 attttcgcaa taccccggga ggttaacctc ttcggggtat tgtgctaata tgtcaaactc
     9901 ctgtagaccc aaaccctcca cctctattgg tgcctatatc cttcaccgac tcaacaacag
     9961 taaacttaat tctgggtaat tcctgaagca cgatctgcgc aattcgatct ccttttctaa
    10021 ttttataagt acccatttta ttgcccttac tttcaagggt taatatttcg ttactgaaat
    10081 taacacagca agccgtatta aactcggcaa ctgacgaatt gctcataata attccaagtt
    10141 catctctata tccgctatca atagttccag gagaatttgc aatcctcaat ggtgttttat
    10201 aggaaattcc gctccttggc cttacctgta tttcaaagcc ctcgggtatt gcaagtttta
    10261 tccccgtagg aattattgct gtttctccag gcttaatcat tacatcttcg gcagcaaaaa
    10321 catccattcc ggcatcacca ggctttgcat actcgggcag aacagctccc tccctgcaaa
    10381 gttcaataaa tacctcaata tccataaata accccctgat ttttcaaaat atgcttctct
    10441 tgatacagca aatccgtatc cctcttatac acctctgaaa tagtgacctc tcgtaaaacc
    10501 cttgtccttt atcctggcaa tctctcgttc atatttatct tcaagtttga cttcaagcct
    10561 attatcaaat ttattatcaa gtctattgtc aagtctatta tcaggcttat ctccaagttt
    10621 atcttcacac cttatatcaa aatgctcatc atctgctaca aaatcattct ttagctgtat
    10681 tttgctattt attaattccc tgtgcatatt tacaagattt acaacttcat cagggctttc
    10741 atcccatata ttaattctga atatgtctgc acctgaattt gcaattttat ctatattatc
    10801 tgcaagaaac aatatctttg aattaaaaat ggtactcctg cagtcaattc tatcacagaa
    10861 catgggaaac ttttcattca ttctgtctaa taatgaatat gtaccctgtg tacatgatcc
    10921 tttacatttt gattcagtta agaccccgcc aattgttgca ccaacagggc agtattcgct
    10981 tgtcataaga ggtattcttc cataaatcac cacttctttt tgcaaattca atccctcatt
    11041 atttccctta ttgcaaatat tttttatttg ttccaaagtt agttcatatg agaaagtaac
    11101 tccatcagct cccatgttat acaagtttcg taccgaaata ctgttgaatg cattaaagga
    11161 ataatcacat gcaactttta gatttgcact tgtgcaaggg cctggattta attttgcaat
    11221 tttcaacaca cattccaaag tcccccagtt tcctgcaaga acaccatcta tgcctgattc
    11281 aacaatccat ggcaaattgc tttttatcaa gctgtcataa tttcctcttg taattgaagg
    11341 caaccataca aacacctctg aaaacctatt ctttatagat tttagaacag tcttcaccaa
    11401 ggaagtttca tctttagctt tatcttcagc tttagtttca gctttaactt tgctactttc
    11461 tttaaagtgc agaaaaaaac ttaagggcaa atatattcta tcagcattta actcagaata
    11521 ataacgatcc ttctgtaaat cgaactcata gaataagact gatattttag gtttacataa
    11581 ttcaacatca tcagctgata ccacttcact agataaaatt tcaatagata gatttatagc
    11641 agaagataaa ctcttacctt tcctgtattg atttcctcta tattgcatta ttctactaat
    11701 ttccagtttt tcaagtgcct gccttcgcag tttattcaat tcctttaccg gaagtgaaac
    11761 accctcatca atgcgaatat caaggttgtc gaattcaaag ggagtccccc ctgtcttact
    11821 tatctgatct cttacccggt cttctgtaag gggtagattc atagctttct ctgcaataca
    11881 ttcaccaaca agttcaaccc tgttttcatg attatctttt acagagaata ccggccggtt
    11941 acccgcttta atatttaact cacctgtaat atgaaccttt ctgccagagc tgccagagct
    12001 atgcaaccag gtttctcttg ctgcttccat aagttttttt tcggaagttc tatatacttt
    12061 atcccctttg cttatgatgc cggttaatcg tccaacatca ataacatctc ccgcatttgc
    12121 ttcttttaca tttatgtcat ttttctttat ttcgcttact actgttcctg gactttctaa
    12181 atcaattcta ttatagcccg atttatcagc ttcttttcta ttcccggatc tcctgttttc
    12241 aaattttcca tatccatcct cattattcca tacttcaatc ccatcaccaa ttgataatgg
    12301 ttcatcaaga cctatcctgg caagttttga tttactatca tatgaaatca ccttcccaat
    12361 ataaatgccc cagtttttag gtttttccat acacatcata tctgccccac acttaccaaa
    12421 caaatagccc tttgaaaaac ctcccctgtt aaatacttgg tgaagatccc gcatatcttc
    12481 cttggataca tgctctttac tttctgcaac agctgcatcc aggtattttc gatagattct
    12541 tactactgta gctacatatt cagggctttt cattctccct tcaatcttca gagatttcac
    12601 tcctgtgcta attatttctt caaggttatc tacagtgcaa atatctttag ggctaagcag
    12661 atataacctg tttttgttgc ttggtacata ttcttctaaa tcatatctct ttttatcttc
    12721 agcacatctc tttttatctg ccattacaag ctgatatgga agtctgcagg gttgggcgca
    12781 tttgcctctg ttgccgcttc tcccgcctaa catgctactc ataagacatt gtccggaata
    12841 tgatatacaa agagctccat gaataaatat ttctatttca gtattacatc tttccacaat
    12901 ataactaatc tcttcaattg ataactctct tgcaagtaca accctcttaa atcccatttt
    12961 actaagaaat actacccctt ccgggttata tacagtcatt tgtgtactgg catggagatc
    13021 cagattggga aatttctctt tcaatatacc tgccagtcca atatcctgaa taattaccgc
    13081 atctataccc atttcacaag cttgtcccac aaggttcaaa gcatccttca tttcagcatc
    13141 cgatacaaga gtattcattg taaggtaaac cttcacatcc cttaggtgtg aataattaat
    13201 agctttttca agctctatat tatcaaaatt acctgcatat tgccttgcat taaatccctt
    13261 tccgcctaga tatacagcat cagcgccatt ttctacagca gcttttaatg cctccatgct
    13321 tcctgcaggt gcaagcagtt caggaagtct ttttttagta attattttat ctttcattat
    13381 ttataatcat ccgttgttat ttaatattct attactttaa tcttctattg tttactcttc
    13441 cgttaatatt aatctcttgt tgttttggcc ttctaatttc tccatggatt ttcttacttc
    13501 ctttagttca gtatcacttt ttacaaaatc aagctgaagc tgtttgtata agccattcac
    13561 atttgaatat tctttagcaa gattctcatt ttcagctttt agcacactta attcttcgcg
    13621 aagtttttta agctcatttt caagcttctc acttttttca taagtttttg tataatcgtc
    13681 tgcaatattt agggctgtga ggacggctgc catacaggta ctaagcttat tattgctttt
    13741 tattatctca cccatttttt tatctacaaa cagccctaat ttatgcatgt attcttcaga
    13801 ttcagtacct ctgaccatat actccatacc agcaattctt aataatactc tattttttgc
    13861 cgtcacataa accaaccccc cagctttatc attatgtgta taaatgttct ttatatataa
    13921 ttatttttta tattagtttc tgcttttgta cgattcaaaa tacatggaat tacattgtat
    13981 ttatacaata tatttatact atgtatttat actatgtatt ttcccttacc atttaagcta
    14041 tatcaatatt atactatatt tcttctaaaa aagcattttt atgatatttt tttatctttt
    14101 atgcctggtt cctagctaaa cctcagtaat tcacctgtat ggggatctat gtacgctacc
    14161 cagtaaccga atgctccata ttttgatctg ttcctttcaa cctggaccca gcggcacaga
    14221 ttcccaaaag ggctgtcatc tatattgtgt gtacccggta taccacatac aaaatagtct
    14281 ctgcctcttt ttctactact gaatattcct gcaattaaat atctatattt gaaataagac
    14341 ataattattg atgggttgaa aattctggga acatttatat cacattgttc tagcaaatta
    14401 ataagattga cggggctggt aaccttccac catttataat cccttcttct tacattaaat
    14461 gggtcatatc ttttgaattc tttatcaaag cattgtacaa gtttattaat atccggcttg
    14521 tttttctttc gctcatagat gttattatcc tgaacagttt gctctatatc aggctgcacg
    14581 ggttgctcca tatcaggttg tgcagttcgc tctgtttcat ccggtacagg ttgttccgca
    14641 tcaggctgtg cagttcgctc tgtttcatct ggtacaggtt gttccgtatc aggttgtgca
    14701 gtttcctctc tattatttga attgctattg aactcactga tacaaccagg atatatttta
    14761 ccgcaagtta attcgtctat tccaggtata ttttctgtaa aagaatactt actctctact
    14821 ttccctgtat atttgctgta aatatcatct actgaatata cttcatgctt attatcagta
    14881 tttgcatcat ctactatact caaaatttca tcaattgtgt gttcttcagc ataattccta
    14941 ttgatttcat attcttcttt gagtggatgt tcttcacgtt cacttgtaga tccgctttca
    15001 tctgtttttt gtttttcgat aattatactt ttctcttttt tagaatggtc tgctgcaact
    15061 tctgcttctt tttttcgctt gacataattt tcaaaggctt ccttccattt cactttacca
    15121 gcactgtatg cagctaaggg gcattgtatc gttccagcat tatagttatc tccatctaaa
    15181 atgacagctg ccatattaaa gtcggtaatc gggtatattg accctccaac atagtttgga
    15241 tcaaaatccc acagtaatag tcctcttccg tcattaacct ggatattgcc tactttcaca
    15301 ggataaattt ccgaatttct atgtctcata atataaatgc tatatttatc cttacccctg
    15361 ttctctttca aattctgtac cattgcataa aatttgcctt tgctttcccg tacttctatc
    15421 cgaacatagc caccaggcac atctttaagt gcgaaacctt tatcttcttc attaagcatc
    15481 catacaaatc ttttaaatct catactttcc atactttatt ctacccccaa aatatatcct
    15541 aataaatcct actatatatt tatatgagca gattcatatg aatagaaata tggtttagga
    15601 taatgttttt tgggaaagca tatttgaaat ctgtgcaaat tgaactattg ataatgtttc
    15661 ccctcttgca ttttcatcta ttccctgact ttttaaaaca gctttaattt cttctttatt
    15721 tagggaaaac atgtttgaat taaataatga atttaacaag gtttttcgtc tttgcccgaa
    15781 agatgccttt acaacattaa aaaacaattt tttatcctta atatatacag gagcaacctt
    15841 atgtcttata agccttacaa ccgacgagga tactttcggt tttggaaaaa agcagtgagg
    15901 cgggacatta aataaaactt ccggggttga aaaatattgt gcagccacag ataatgcgcc
    15961 ataatccttt gtaccaggtt ttgccagtat tctgtcaact acttcttcct gtatcataat
    16021 aacaataata tctatagcgt tctctatagt caggagcttc attatgattg gagtagtaat
    16081 gtaataaggg agattaccta ccacttttat actattaggt ttaaatccat tcataataat
    16141 ttctttttct atatcaagat ctaaaaagtc ggtatttatt atttgtgtat tagaataatc
    16201 ttttaataaa tccgtaagtg ccggaattaa atgcttatcg atttctacag ctatgaatct
    16261 tcctgctttt tcagctatat cctttgataa tgcacctaaa ccggcaccta tttctattat
    16321 taaatcatca ttttttatat ttaaggcttc tattatacct ctacaggcaa aatcatcaac
    16381 taaaaagttc tgtcccagag attttgtcgg ctttatgtag tatttattta tagtctcttt
    16441 agtgcctgct cccatactaa tcccctgtca attgccaatt ttccaaatat acttttaatc
    16501 tgccagaata tatactttaa cttttcttat accccaatta tcggcttcct tctgggtatc
    16561 catataaagg tctaccatat ttccttttac tccgcctcct atatcagcag ccagagcata
    16621 tccgtagtcg ggagtcttcc ctataccttc aatatatacc cttgaaccca gcggtattac
    16681 tttagggtca acagcaataa ttcctttttt aacttttact cctgtgtaag ttattccgaa
    16741 gtagggatga tccggatttt tgccggtatc tttgaatgaa gcagtgtatg cggttgcacg
    16801 cgcatcaata acttttttgt agcgaaactt ttctcctctt gaagtagtac cagtggcaat
    16861 agtgccatat tcgacaactt tatctatagg taccatggct atagattctt taactaattc
    16921 ctttgttttg atttctccat cctcataaac aactctgtat aaatattcct ttacaccttc
    16981 tttaccatcc cttactgtct tttcaacacc ctgttccata cgctggctag gccttttttc
    17041 cactttgaaa gaaattgctt ccttctcagt tatgaggtct tcttttatcc tgataattct
    17101 aaatttcatt cctgggaaaa cagtatcttc aatatttgct ccttcaagaa ggtcactttc
    17161 gctaaacgct atctgatttg cttccaaaac tcctttgact gtattcattt gagtaagtat
    17221 ctgttcttct ttaccgtcta catatatgct tacgggggtg gcacgcttaa taagtatttc
    17281 attgtttttc catgatatca gtttttgttc ccctgaaggg taaacataat cttccttttt
    17341 aacatttatg ccattttgct gtaaggcttc ttcaattgtt tcccctttag tttttaaaat
    17401 aacaatactg ccatcatcat tgatagaaac tgtcttcaca ggtgctttca atacttccgc
    17461 tccggcaaat gccgttacaa cggaaacagc aataataatt gaaactacct taaataatag
    17521 ctttatcccc atttcatcaa taatttccct aattcccctg ccaattaagt ttttgccaaa
    17581 gataactttg tcaaaaaaac tttttttaat aaaatgcatt tttgttctcc tcccaattta
    17641 tatatatttc ttttgggttg tagttattct attcaaaaat ttcttccttg tcaaattttt
    17701 ttacataata atccatatta cggtttatat aatagtatta tcaaatattt cttttttata
    17761 cacttaacat aaaactggaa acatgcatta atgtaaatta tactttccta tttatgaaaa
    17821 aggcagcata caataaatgt ttgccgcctt tccttctatc atggattcct aatatccgat
    17881 atataccact ttttatcttt agcattgtaa ctaagaacaa ttattttttt attttccccc
    17941 tgattaaata gcaaatcata cttaacagcc cattcggcca gtttcacatc caactccaca
    18001 agaagaaata catccccctt atcatattca tctataatct ttctgtctat gttatcctta
    18061 tccatgttct tacctgcatt tgtatctata ttatatggag ttatggattt tatcttaata
    18121 ctgttaacta tatttttata cttatttgtg aattgatcaa gactctcttc agcttccttt
    18181 ccatttccat accatatact gtaagcctct ttataatttg ctgagcttat atactccgcc
    18241 cattttttaa ttgaagtctc aataggtccc ccttgctgta tatccttata aacactgtta
    18301 atcacttccg ccttatgcct tatcatgtta ttcaaacgga tattttcctg ctttaattct
    18361 tcaatatcct ttcgcaaagc gttattttca ttggttattt tatctatatt atcattgcgg
    18421 agcttaaggg tgttttcaag attttccaat tgcctgttaa gggcatttat agttaaattg
    18481 ccgcttgtgc ttgcttcttg aaggcctctt atatcttttt ccctgttttc tctttcccat
    18541 agtaaataat tcagccctac aaatataatt ataattaaaa cgaaagacat aataactata
    18601 aactttttca tatctttaca cccccaagct aatggcaaat agcccacctg ttttttattt
    18661 tatattaaac aatcttttgc cattttccat tgtaagctcc gccatttttt caaaagtcga
    18721 tcctttaatc tctgcaatct ttttaactat atattcaaga taaccagaat gattcctttt
    18781 tcccctgaaa ggttcgggag tcaggtaagg acagtctgtt tctacaagaa tcctgtcttc
    18841 tggtacaaat cttaatacat ccaatacttt ttttgaattt ttaaaggtaa ccggtccccc
    18901 aaatgatata taaaagttat tgtttaaaac atcccgcgcc atttccacac tgcctgaata
    18961 acaatgaaac acaccgcctg cgtcccttgc gttttcttcc ttgattattc tcataacatc
    19021 ctcatgtgaa tctctgttat gaattatgat tggaagtttt actctttttg ccagacgtat
    19081 ttgttctgca aaccattttt tttgggtatc cgccggtgta aaatcataat aataatccaa
    19141 tccgatttct cctattgcca caactttatt gcatgatata agactcttca gagaagccat
    19201 tgtactatca tcggtttttc ctgcgttgtg aggatgaacc cctgcagctg catacacaaa
    19261 atcatatttc tcagccagca taatgctcat tttggcagaa tctatatctg aagcagcatt
    19321 taaaatataa gatactcctt cccggtggat tttctcaagt aattcatatc tatcttcatc
    19381 aaatctttcg tcatcatagt gtgcatgggt atcaaacagc atttatattt tactcctttt
    19441 tattgattat acatcagatt gaatgcttgg gcaatctgca gttttacctg gtaattcctc
    19501 ctttagagtc ttcaaacctc aaagcgaagc gagtttgtat tcagcttatt ctggagcctg
    19561 tttcaatttc tccatccact gtcacaagca caagatcctt tttatcgtta gacgccgcaa
    19621 gtatcatgcc ctgggattca attcccctta tacgtacagg tttcaggttg gctgccaaaa
    19681 taacatattt acctttcatg tcttccgggc tgtagtattt tgcgattccc gaaacaacct
    19741 gccttgtttc atttcccact tccagcttta gcttgagtaa tttatcagag ccctctactt
    19801 tttcggcttc agctactttt gcaactttta gttcgacttt ggtaaagtct tctatagtta
    19861 tggaattgga ttcaggctcc ttgattttat cattattttt cgtattttcg tcttttccat
    19921 cgcttttatt tttcccgcaa tctttatttt taagctgagt tccactgttt gcattttcca
    19981 taatttcaag attctcaagt tcctttttaa ggtctattct cggaaatatt acatcaccct
    20041 tgtttacatg aatgccatca ggatacatgc cccaattttg tgcactttcc catgaaacca
    20101 attcctcttt gccggctata cccaactgat gccatatctt ttcaggtgtt tctggcataa
    20161 atggttgaac aagcactgaa ataatcctta tgctttccgc caaattatac agaaccgctg
    20221 caagtcgtcc cttgttggac gtatctttcg ccaaaaccca gggcattgtt tcatcaatat
    20281 acttattggt tctggaaata gctttccaaa tctctgtaag agcagtactg aactgcagtt
    20341 tatcgagaag ctcttccaca ctcccggggg tttgtgcaat cacatttttc aaatcattat
    20401 caaaatctcc cgccgttctc tctgcaggaa ttactccatc aaaatatttt tctatcattg
    20461 ctatagttct gcttaccagg ttgcccagat cattagccaa gtccgaattt attctgttaa
    20521 taagtgcctc attggaaaaa acgccatcag acccaaaagg cacttctctt aaaaggaaat
    20581 atcttattgc atcaagtccg tatttcttaa ttaatacaac gggatccacc acatttcctt
    20641 ttgactttga cattttcccg ccttccagta gaagccatcc atgaccgaaa acctgttttg
    20701 gtagaggctc tccgagggcc attaacattg caggccatat aatagtatga aaacggacta
    20761 tttccttacc tacaagatga acatcagcag gccagtattt cctgtaatcc gaatcattat
    20821 ccgaaagata acccaatgca gttatgtagt ttgaaagagc atctatccat acatacacta
    20881 catgtttttc gtcaaatgtc acgggaacgc cccaattgaa agttgttcta gaaacacata
    20941 aatcctcaag cccgggtttc aaaaaattgt taatcatttc gttttgtctt gaaacaggct
    21001 gtataaattc aggattttcc tctatatgtt taattaacct atcctgatat tttgatagtt
    21061 taaaaaaata gctttcctct tttgtcagtt caaccgttct tccacaatca ggacattttc
    21121 catccaccag ttgtgtttca gtccagaaag attcacatgg tgtacaatac cagccttcat
    21181 actcactttt ataaatatct ccctgatcat aaagcttctt aaatattttt tgtaccgctt
    21241 cctcatgata tttatcagta gtgcgaataa accggtcatt ggtaattttc atggcactcc
    21301 aaagttcctt tattccgctc acaatataat ccacatattc ctttggtgta actccctttt
    21361 cctgagcttt cctttgtatt ttctgaccat gttcatcggt tcctgtaagg aacattacat
    21421 catagccttt taatctttta tatcttgcta tagtatccgc tgcaacagtt gtataggaat
    21481 gtcctatatg aagcttatca ctcggataat atattggagt ggtaatataa aatttctttt
    21541 tttgcattat tgttacctcc taaaacaatc aaatatttaa caatttattg tcagtatttt
    21601 attaattatt ttattagtta ttttattagt tatcttatta gttatcttat tagttatctt
    21661 atcagttatc cggccagtta tatatatatc ttgattctgc tgtaacaact ctgttacaca
    21721 tctttccgat actatcgaaa ttttgttgaa acatggcttt ctgcagtgga atcatatctt
    21781 tatttatata aaattaccca aattccctta tcttataact ataccatttg gtactgcact
    21841 ttttcaagga atttatacat gattctactg caataacttt ccgatgctat aggaattttg
    21901 ttggaacatg actttttaca gtaaaatcat acattaatag tctgtatttt acatatattg
    21961 attataaccg atttcattgg acatttgttt attttttggg gacatttctc tttctggaaa
    22021 tatattgatt gaaattttgc taaatgatat gattttttag tactgagagg gggatggatg
    22081 aaataatgta tagtgcaggg aatttactta ttaatctggt tttcaaatct attccggaaa
    22141 tacgaaagca gatgaaagct caacggaaaa taattgaaga gttatttaat acatttacat
    22201 tcattgataa cataaccggt tacaataaca gtaccgacag taaaccatat agtgtttcac
    22261 ttgctggatc acatttggat ccatacaccg agtcatgtac cgaattatac acgaaacaat
    22321 atattgaacc tcatgttgag ctgtatactg taccggagaa gcattttttt gtaaataaag
    22381 agtatgtgca ggctttatat tctctctttc ctaatgtaaa ctcaaaaaag gtaataaact
    22441 ttatgatatc atttaatatg cttaccatgt atcttgatca gttatgccat aaagaacaaa
    22501 taaaagatga gtctaccgta cgacagttct acctttccat gcttgatgcc gttgatcctg
    22561 atagagaaat cagcaactac tgcaaatatt tcccgagtaa gtcagaaaat aattacataa
    22621 atcaatttgt cgaatgctgt agaaatgaac tggaaggaat accatcctac aaacttgtca
    22681 aagaaaatat aaagaaatat gtctattttt actctgattt ccatacatat aaacatatat
    22741 catatggtga aaatcgtgac gactacctat ttacatggtc tggctattat ttaggccaat
    22801 acgccgatat ttacccatgg gaatttcaag cagcggcttg ttcatgcctt ggaatatttg
    22861 ctttattatc ttctgcattt gatccctcac tgactgatga aatgatttcc agtattgaca
    22921 aggcctactt tccctggata aatggtttac aggtgcttct agcaaattat ttgtgcttgt
    22981 atcatgatat tattaatagt aatttaaact ttacaaatta ctataaaaat ctcaaactgt
    23041 gcgaagaaag gattattttt ttcttcaaac aatctcttaa agcatgttcc cagggaaatt
    23101 tatctgataa tcatctttca gtagtaaatt taattttagg tatatattta tcagacccaa
    23161 gagccgacct tggtttaaag aaaataacca gtaaaaatct gattaaaagc ggagatatat
    23221 tatatcacag gttattgaag cttattatgt tcttgtccct acctcccaaa tatcaatgtg
    23281 catatccata ctgaggctaa tattcctgct gcatcagcaa ttaatgcagc taacagggtg
    23341 tgctttatat tttttattcc tgcagctcca cagtatacag ttaaagtata aaatatcgtt
    23401 tccgtagaac ccatcatggt agatattaat cttcctataa acgaatccgg cccatgcacc
    23461 tttataatat ccgataccaa agctagagat gcacttcctg atataggcct taacaatacc
    23521 aatggtaaaa cctcagatgg tatgttaaat aagtctgcca gaggttttac tgaaaaaact
    23581 aaaaggtcca ttgcaccaga agctctaaat actccaattg ctgcaaacag tccaaccaga
    23641 gaagggataa ttctcaatac agtataaata ccatcttttg cgccttctac aaaaacatca
    23701 aaaactttta tatctctata tagtccaatg attaaaatta ttagaaatgt tacaggtata
    23761 gcaaaatcag ctatcacttt aatgatcctc atctccccgc cccttttctc gcctttttta
    23821 cttttctccc ttttccccat atacgtgaaa gcagtttaac tgaaattaca ccacaagttg
    23881 ccgcacagac agatgcaatc cacacagaac ttattatttc cgcaggattt gctgaacctg
    23941 cctgggctct aatagctatg accgaagtag gtataagttg taatgatgct gtatttaaaa
    24001 caagaaacat actgatgtca tttgaaacgg aatgttttgc aggatttaac ttctgaagct
    24061 cttccatagc ctttaaaccc agcggagtag cagcatttcc aagccccaaa atatttgcgg
    24121 acatgttcat aataattgtc catgcagcag gatggttttt aggcacatca gggaaaagaa
    24181 aatgtattaa cggtcttacc aatgaagcaa gatttcttac gagaccactt ttctcagcta
    24241 ttttcattaa tccggaccaa aggcatataa tacccagcag gttaatactg agtttaattg
    24301 catcccccga cgattcaata accgcatttg tcacttcagc aattctacca ttcattattc
    24361 ctattataaa tccaaataca agcatggcaa accatatata attcagcata cttcatcaat
    24421 ccttatcata taaattaggt aaattgggga catttatcct ttgttcacct gccctttgca
    24481 tctgttctat actttagggc atctctgtcc ccttgcatta tatatattta tgtgactata
    24541 tacttccata ttactgcctc tgatacaaaa tgcacccttc aataaacttg tactttatta
    24601 tttatgctaa atatggtata atttagaata gtattcaaca gttgtttgac cataaacaat
    24661 aatctaaaat ataaaataaa ttttttttta ttttttaatt ttcatattga cgattattgg
    24721 tatatatggt actattaaga tatgaagttt gtcgaaattt gtaatattgg ggaggaagag
    24781 tactatgaaa tctacaggaa tagtaagaaa agttgatgaa ctgggtaggg ttgttcttcc
    24841 aattgaatta agaagaacac ttgatatcgc tgaaaaggat gcattggaga tttacgttga
    24901 tggttcaaca atcatattga aaaaatatga accagcatgt attttctgta acgatgcaaa
    24961 agacgtagtt tcatataagg gcaaaaatat ttgccaaaac tgtatgaaag agttgaagaa
    25021 ataagttttt cttatctaca atttaattat ttaagatcac ttgataaagg gccggaatat
    25081 attcggccct ttatactttt atgttttttc ttcgattaag ctctgataaa cctcacgtct
    25141 tgatacacct ctatcctcag caactttttt catggcatcc tttctgctta ggccttgatg
    25201 cagatatatt tccatgtgct tttttatgtc cgtatcctcc cacagccttc tttcttcatt
    25261 aacagcggaa ctatcatcca caccttccaa aattattaca aattcacctt taggactttc
    25321 ttcttcatat ttactgatag cttctgaaag ggtgcacctg attaactctt caaatctctt
    25381 ggtcaattcc ctggccagga caatttgtct ttcccctaaa atattataaa ggtcttttaa
    25441 agtgtatata agcttatgtg gggcttcata taatattatt gttcttggtt cgttttttag
    25501 aaacattatt ctttctttcc gtgacctttt attcatagga agaaatccct caaaaacaaa
    25561 cctgcctgca ggaaggcctg ataatacaag tcccgttact gctgccacag gacctggaac
    25621 catagtaacc cttataccat tttcaattgc aagtcttaca atgtcttccc ccgggtctga
    25681 aatacccggt gaacctgcat ctgataccag ggcaatgttc ttcccttcaa gaagaaggtt
    25741 tagcaggtaa ttccctttag ttgccttatt atgctcataa tagcttgtta aggatttttt
    25801 tatatcataa tgattaagaa gttttattgt ctgccgtgta tcttctgcag ctattaaatc
    25861 aacttcctta agtattctca atgctctcag tgttatatct tccaaattgc ctattggcgt
    25921 tgccacaaga tataaagttc ctttttcatc cggtttagct ttttcgtcaa gtttagcttt
    25981 ttcgtccaat ccaaattaac ctcttattcg tcaaatttat ttaacaagga gggtgcgtaa
    26041 tctaatctat tctaatctaa tctattctat tctattctat tctgtgataa attctattaa
    26101 tttcatccga ataattaccc tcagcattat atacatataa gggatccatc acctttaacc
    26161 ctgcatttcc acctctagta ccctgaacaa ggaagagatt cggcttttta ttaggagatg
    26221 ggtggacaaa tcttataaat ttaggttcaa ttgagtatag cctcataaga taaagtacat
    26281 ctacaattct ttcaggtcta tgtaccattg ccagacgccc tccgggtaca ataagcctgc
    26341 tgcatgcctt aatgatatct tccagagtgc aggttatttc atgccttgct acagctttta
    26401 aatcaaggtt gttgataatt cctccgcctt tgttcatata aggtgggttg caaacagcta
    26461 gattaaactt tgaaggtcca aaatactcaa ggccattttt aatatctccc tgcacaattg
    26521 ttatacgatc ctgaagatta tttaatttaa cacttcgctc tgccatttcc gccatttctt
    26581 cctgaatctc aagacctgta attttgccgg cttctgtttt tcctgcaatt agaataggta
    26641 ttattcccgt ccctgtgcca atatcaagga caaggtcgcc ctttctgatt tctgcaaaat
    26701 ttgccagcaa cactgcatct acaccaaaac gaaatccatg ctctttttgt ataagcataa
    26761 gtcctttata ctgcaaatcg tctatagttt cattttcctt aaggaatact tccggattgt
    26821 ccctcatata cctacccttt gaaaatgaga aatcaagcaa ggattaagac ctctcaccgc
    26881 cccccgcttg attctctatt ttacgacctg tatttatatg ttacataaac caaaccgcca
    26941 tcctgcttcc tataagcagt ctcttatttc tgctgaggag catctacagg gcaaacacct
    27001 gcgcatgagc cgcattctat acaagcatcc tcatcaataa catatttccc atcgccttct
    27061 gaaatgcatg ataccgggca ctctgattca caagctccac agctgatgca ttcgtctgtg
    27121 atatagtatg acactgaaaa cacctcccca tacttataaa tcatatataa agcaaaacca
    27181 ttgaaacaaa ccttcgataa acagtatatt acgtaaggtg tttacagtca atagatattg
    27241 ttaatcttcc agctctttaa gggcttcaag gtctatatcc tggtcatcct gcggcattgc
    27301 tgcgtcttta attattctta tctcgcttaa atcaaatatt tgcaggtcag tttcatttcc
    27361 tttgtccagc ttgactttta cattttcctt gatcagattt atctccatta caactccctg
    27421 cccatctgga gtttcaacaa tagctccagc cttaggtgct cttttcagga tttcttcata
    27481 ggcttcatgt tcatacttca ggcagcacat aagtcttcca cagcaaccgg atatcttggc
    27541 cggatttaaa gataacccct gttcctttgc catttttatc gataccggct gaaactcaca
    27601 taagaatcct ttacagcaaa gaactctccc acatatgcca actccaccca tcatcttagt
    27661 ttcatcccgt acacctatct gtctcagttc tatcctggtt ttaaatatag cagccagatc
    27721 ttttacaagt tctctgaaat ctatccttcc atctgcagta aagtaaaata atatcttatt
    27781 attgtcaaag gtatactcta cattaatgag cttcatatcc aggttatgct cttttatttt
    27841 tttaaggcaa atttcaagag cttctttttc ccttgctata ttctcattat gatgtttaat
    27901 atcttcttcc gttgctatcc ttataacttt ttttaaagga gccacaatct cattttcagg
    27961 aacctctcta ttggccagaa ctacatgtcc aagttcaact cctctggcag tttcaactat
    28021 tacaccaaca ttttccggta tatccagatc ttccgggtca aaataataaa tttttccagc
    28081 tcgtctaaat cttactccta caacttttac cacttatatc aaatctcctc ccgaagtttc
    28141 attatcattg tttcaacagc taactggtaa ttggcattct gttttatact gcgtcgtgta
    28201 aactcaattg cttcaatgtt tcttaccagc gtttgaacag aatactttcg cgcagcttct
    28261 attattttac tttttttatc tgaattaatc aacatgtttt cttttccatt tacccgggcc
    28321 attaataaat ctctataata tatagacata atatctaata aattatctat attactttta
    28381 ttatcatcaa aaaagtcaca aatatcgaaa tattccatcc catctaaact actcaacttc
    28441 agaagaattt caaatgcttt ttccctgaga gatgcaaatt cgctggatac tgccaattct
    28501 aaagcagttc ctataacccc atcagagtaa tgaacaataa attccatttt ttctgcatct
    28561 ttaacaacat ctttagagtt gccaaagtca tctatagtct cttttaaaga atcttgataa
    28621 aactttgatt taagtacagc tctaacctca ttatgggagt acttgccaaa ggtatatttt
    28681 gttcctcttg aacgcacggt tcccaataaa gcttcgtaat tagccgtagt aagaattata
    28741 actgtgtatc cgggaggctc ctccaaagtt tttaacagac agttttgagc ctgaatggtc
    28801 attttatcag catcagttat taaatatacc ttcctatccg aatacaaagg cctcacaaca
    28861 attttagcct gcatactacg tatatcatca atagaaatat tggcatcaga tgtttctatt
    28921 tcatagaaat ctggattgga attttcattc cacagtatgc acggccgaca cacgccacag
    28981 ggtccatttt ctccgggcat caagcataga agcatatgtg caaaagctct ggcaacagtc
    29041 ctttttccta tgccttttgg tccactgaat atataagcat gtccaacctt attatcaata
    29101 acagcttttt ttagcttacc aataatttcc ttttgtccaa caatttcgtt ataatccatc
    29161 ttatgctcca cgttttactg ctttaattcc atgtattttt acatcatcta ttttcacatc
    29221 agcatgtcta atataagccc ccggatataa tccaggcgct gtaatacttt taatttatcc
    29281 tgctggttct ccaataagtc ctttgcgaga acgtccagtt ccgaatttat ttttttaaca
    29341 acagagtatg tcctataatt gcctcttctg tccatcatat tttgccttga gaatttatag
    29401 gaattattca ggaactcatt cataaactct gaaatagacc ttttataaac aatatattct
    29461 ttaatatcca ttctcttttc aagcttttta ctctgttcga atattctatc agccagctgg
    29521 ttaagctttt cttcataact tttatactct gcttgcttca ggcttccctg aaaggtttta
    29581 ttgtcggctt caacttcttt ttttacatca cttattacca taccggtcaa gttgggtctg
    29641 ttattcagag tttcttctat tttcattcct accaccccat ccaggcagaa acatgctgct
    29701 gaaaatacat ggttatgcct tctcgaacct gtcaacatta atcacaaata ttgttgctcc
    29761 tcctacaata acctccacag ggtatggcat aaacatacct cccatattat tgggcgtcat
    29821 tgtagagttt attgcctgct ttctgctctt ggatttctgt ttaataatat taataacagt
    29881 atccagtttt tcctcagtta caccaactaa taacgtagta tttccggccc gtaaaaatcc
    29941 acctgtggaa caaagcttag taacactaaa accgtttctg ttcaattcat ccataacttt
    30001 cgggccatct tcgtcatgca caattgcaaa aaccaatttc ataaaacatc ccccttatat
    30061 gtcatttatt actatttaat aatattattt aatagtatta tttggtacta ctatttaata
    30121 ctataatgta ctgttaagca catgatgaag gttcctcttc actatgctaa aaacatcctc
    30181 cggactttta tctgtgctaa ttattttaat cctttctgga tataaagcag ccaattcttt
    30241 atatccatta tataccctgg tgtaaaaatc caattcttcc ctttctattc tatccggagt
    30301 tgacgatgca aatctccttt ttagtgctgt ttcaggtaca atatcaaata aaaaagtaat
    30361 atccggcatt attccacaaa cagcaacact attaactctg gttatcaatt caatgtctat
    30421 gcctcgtcca taaccctgat aagcatagct ggaatcaaga aatctgtcac atatgacaat
    30481 tttgccctta tctattgcag gttttattaa ttcatgaata atctgggctc tagcagaagc
    30541 atagagcagc atttcagtta cctgatccat ttcactgttt tgggggtcca ataatatgga
    30601 acgaatcttt tcaccaatac ttgtgcctcc cggttcacgt gtaactataa cttcatatcc
    30661 tttttcctca agataacttt tcataagacc aatctgtgtt gtcttacctg aaccatctat
    30721 tccctctaca ctaataaata agccccgctc cattattata attcctccag atatattata
    30781 tcagtagttt tatttccttg attgtattta ttataccata ttattctaat ataaacattc
    30841 ttttttatgc aaatcatacc tgtttcaaag tttacatcat actatcatac aatatccacc
    30901 acatcaactt catcattagt atctattcca ttaacactgc cacccgcttc aataacgccg
    30961 tatatatatt ctacaatatc gccgctgata atttcacccg gacaaactac cggtatacct
    31021 ggaggatatg gtacaataaa atctctgctt atttctccca tggaattttt taatgcaatg
    31081 tattttcctt tacttcggga tacatcctga agcttcatcc cctgtttggg aatgggaagg
    31141 ttttttgtag cactagtggt ggttcccagc aatattccaa ccgatctttc aactttatta
    31201 tccttttcat gggcaatctg ctttaaagcc aggtataaag cctcaatttc ctttcttccg
    31261 tcagctacgg ttgctatgca aacaatatta tgcaagtctg ccatctccac ctgtatatgg
    31321 aacttttccc taagggtttt cgctgtctcg tagcctgtca ttcccaattc tctgacattt
    31381 ataacaactc ttgttatgtc tttttcaatt ttcataccat cccaactaat taaatccttc
    31441 tcctgaagaa cttttatgcc gcttattaaa ttagaagcat ctataaacca ttcaatatta
    31501 tcaataagtc tttccagaag ctcgccacca tgtgtttcca ttatgctccg ggctatgtct
    31561 aatgaagtca taataatata tgacgggctt gttgtctgca gcagccttaa attgaattca
    31621 agtctgtcaa catcaatcaa atgggatcct aaatgcaaat atgccccctg ggtaaatgcc
    31681 ggcagcgtct tatgtgcact ttgtatacat atatcagccc cagctgataa agcacttgca
    31741 ggaagagaac tgtgaaaatt gaagtgtgct ccatgcgcct catcaacgat taaaattttt
    31801 ctataggaat gtgttatttg tgcaattctt tcaatatcgg aacacacacc ataatagttt
    31861 ggcctggtta taagtactgc agcagcattg gggttctcct caagtgttct ctccaattct
    31921 ccggtgccaa caactaatgg tattccaaaa gaatcatcca ggcctacagg cacatacacc
    31981 gggttggcac ctgccagcat catcccgcca ataacggatt tatgacaatc gcgactaaca
    32041 attattttat ctccaggcct gcagaccgac attatggcag catgtattcc gcaagaagac
    32101 ccgttaacta aaaagtaagt ttttactgca ccaaaagcat atgccgccaa ttcctgtgat
    32161 tcctttatag caccgatggg gaagtgcaga ttatcagtac ctggtatctc cgtaacatcc
    32221 aattgggaaa ttatagctgc atactcaggg aaaataccct ttcccatttt atgcccgggc
    32281 atatgaaaag gtattggatt ttctcctata taatttttta acgcttcata aattggtgca
    32341 ttaattcgag tttccttaaa gtgattcata gtcccaactt caagcttctt atttctttaa
    32401 tctcaagtct tatttccttt tctatcctaa caatatcttc ctttatatca tctaaagttt
    32461 catcagttct gattctttta ttataattcc gggatgcata ggcctgtggt ttttcccgtg
    32521 atttctcctt ttctctaaat ccttcttttg atatgtctcc tagcattccc ttcgctttct
    32581 ctcccggtcc ttctttagtc ctttcttttg gtatttcttt catcctattt ctgtgcacct
    32641 ctttgtttct ctcttttgaa acctctttta tcctattcct atttccagga gtttccatag
    32701 ttttctcttt cagaaatgtc ttccctcttt ccttaagaat ctcttttgtg ttctctctcg
    32761 ttttctctct tgcattttcg gtttgcgttc cctttgttat ttcattcagt gattcttttc
    32821 catgatatct gttttgctga ataaaatctt ttcttcgttc ttgattccca gggtatttct
    32881 gctgtttatt atttttctgc cggtcctcat attctcccgt tctctctcga gtatactcct
    32941 gattctttgc cacgtaaacc tcaccctttc atgctaaagt tgttttcacg ggaattaatt
    33001 actaattcta ctgcactaag attattacta ttctacatag caatccatac ctcttactga
    33061 aacttctcca gatataaatt gatgtgttgc tccatcctcc cctttaacca ctaatttgcc
    33121 atcttcagta atacccaggg ctattcctct gtattcaccg ttttctgaaa ctactcgtat
    33181 ttcctttccc agagtaatag agaattctct ccacttttcc aataatacat ctgttcttcc
    33241 atctttgata tagttataag attcctcaag gttttccagt attcctttta ttatatcact
    33301 cctcctgaac catttgccag atataccttc agaaagttta tgaattttaa gtgatgttgc
    33361 ttttccccgc aattcagggg ggaagtcttc ctcatcatga ttaacattta atccgatgcc
    33421 caatattata tggtttactc tatcaagttc agaactcatt tctgttaata tgccgcagac
    33481 tttctttcca tccagtataa tatcgttagg ccattttatt cccgtactaa tacctgctgc
    33541 ctgcataatg cctgatacaa ctgcaattga tgcggccagc gtaataactt gaacctttga
    33601 aggcagaata gcaggtttga gaaccaccga catccatatg ccttttctat cttttgagtc
    33661 ccaagccctt cccagtctac cttttccagc tgtctgtaaa tcggcaacaa caacagttcc
    33721 atctttgcag ccttcatgtg caattttcct ggaataaata tttgtagaat caacactctg
    33781 taaacaataa atttctttcc ccaagcactc tgtatgtaaa ttatatccta tctcataagc
    33841 atttagtata tcaggtatgt caaccagcac atatcctttt tttgaagaag attcaatatt
    33901 atatccctca accttcaaat cctttatatg tttccaaata gtagttctgg ttacccccaa
    33961 ttctgagctc aacaactctc ccgatataaa atttccctta ttattcttta atatttccaa
    34021 tattttatat ttcattttat taagtctcca ttactatttt gtatatccac aattattttc
    34081 cactattatt ttctatattg catatggttt caggtatttt aatggtatat attctttcat
    34141 ttccattatc ggtattatcg ataaatacag caatttttct atttttatct tcaattttga
    34201 caatttgttt taattcagat aacttgcagt aaaaaaaata catggttatg tcatcgaaat
    34261 cataatattt ttctttatta aattctataa ggttgtttaa ataaagttcc tctgaactat
    34321 taaataaaac cggagtatcc tgtgccttat ttaaaagatc ggcctgacct ggaataacag
    34381 tagtggtaac aaggcattca ggtaaacgat atgagccgaa tatatacttt tcaaggggta
    34441 ttatggattt aaggtagagt tccattactt cgggcatatc cacccctgcc tcataaaggt
    34501 atctgtctgc aacatagcag taatctggcg gtaaatccag tttaatacac ttataggctt
    34561 catcattgaa cttgtttata tcatccttcg ggtttaataa tgctgaaata cactttttga
    34621 tgatgccctc aatcattatc tctttgctat gccatactga aagctttagt ccgcattcaa
    34681 ctatattttc ggattcttca gccggcacat agtaatacac ttctgccaca aaatcccctc
    34741 ctttcaatat acagccctaa atgacttatc tatacattat tataaatgcg tacttcttct
    34801 ttatcatcgc atttttcaat aaaataatca attcctatat ggttaaattc catatcggga
    34861 gcaatttccc ttaaaggtat cagtacaaat gcccgcccca gcatttcggg gtgtggtata
    34921 gttaatcttg gaaggcctat atttatatta tcataaataa gtatatccag gtcaatagtt
    34981 ctcggtcccc aatgaattat ccttttcctt ttcagcatat tttcgattcc ctgcagctta
    35041 tcaagaagtt caagtggttt aagaccggtt tttgtcataa taaccatatt taaaaattta
    35101 tcctgttctt tgtagccaac tggttctgtt tcataaatac gggacatttt cactacagaa
    35161 gtcttatcca gtttggaaat tgcattaatt gcccgttgaa gattttcaac ccgattgccc
    35221 atatttgatc ctagtgcaat ataagcttca gattccattt ttatcctccc taaacaagtg
    35281 cgcacactgt tccatttcac tttctcttcc tcttgaactt tctcttgctc tttgtctttc
    35341 tctttgtttt tctctttgtt tttccctttg tctttccctt tctatttctg ctcccatcca
    35401 gtcaaactct ccctctatgg gtgcataagg cttttttacc agaactttta cttttttaat
    35461 attgttatta taattcaata tggtttgagc aataacctct gccagttttt ctattaggtt
    35521 gaacttgtta ttttcagtaa tatacttaat tctgctataa acttcggcat agttaactgt
    35581 atcatccaga ttgtcagtat tacctgctct acttaaatca gtatatattt ccacattaat
    35641 aaaaaagacc tgacctttta tcctttcctc gggtaacact ccatggtatc cataaaattt
    35701 catatttttc ataataatct tatcaaattt atggatttta tctgttttat atgctttttt
    35761 ggcgtatact tttctgcatc tactcatttt gaaaaccctc tgacaattgc atctgttacc
    35821 ttagcaacac gatacatttc agccacatca tgaatcctta taatatcagc tcctcctgca
    35881 ataccaaggg caacagttgc agcagttcct tcaattcttt ctgtaaccgg taaatttaaa
    35941 atattaccaa taagagactt ccttgatgta cccaataaaa ttggaagttc caaatctttt
    36001 agttcatata atcttctcat aatttccagg ttgtgctcaa aagttttgcc gaatccaact
    36061 ccggggtcaa taatcattct ttccctgttt attcctgctt tagttgcaat ctcaatactt
    36121 tttataagga aagacttcac atccttcatt aagtcatcat atactttatc atccttgtta
    36181 tgcatcatta ctacacccgc attatatgcc gccgcagctt cagcaatcag aggatctttc
    36241 tgaagtcccc aaatatcatt aacaatgtgg gcccccgcct gtagggcttt ttctgctaca
    36301 acacccttat tggtatctac agatatgggt actcttaacc ttttagccag aatttctata
    36361 acaggtacta ctcttgatat ttcctcttcc gcatctacag gaatataccc tggtctcgta
    36421 gactcccctc caatatcaat aatgtcagca ccattctcaa ccatttccga agctttttct
    36481 actgctgctt caactccgga gtatttgcca ccatcagaga aagaatcagg tgtcacattc
    36541 aatatgccca ttatatacgt ttttttcccc aggggaagcc tatagcccct gcattcaaag
    36601 tatttcaact taaaagccac ctctgtcgta aattattttt ataattataa ttattatatt
    36661 ataattactt aacttgcgaa aggttttccc gataaatttg gtgcggaaat aatttaaaaa
    36721 tgaattcttt gtctattttt gctaaaaatt ttatataaaa tttattttca ttttacctag
    36781 aaataaaaca ttcacacata taaaattaag ttattggtaa tatcataagt gtatcaggtc
    36841 ttattgatta ttaaatattt ggagttgtcg aaatggtaga tctatttaaa ctaaatacag
    36901 atgaactaaa ggcattagtg atctataagg gagtccttga agaaggggaa agattcccaa
    36961 aagaattttg gaccggcgaa aaagagcagg ttaagggcat aaaaataaag tgccgcgttc
    37021 ttacccgcta ctgctttgaa aacctttccc gaatcgaagt agcggattta tcgaaatata
    37081 acctgaagca attaaagtcc cttctggtta aatataagct ttcaggtatg gtacagcgtg
    37141 tgttcaacca cgatgtggta gctattctta aaaatgcata tcctgaagaa ttcaggacaa
    37201 gaaagctaaa agagtggatg tggagcaaac atggcatatg gcatgatgat aatgcaatta
    37261 ttgaagccat acatgatatg gtcaggaaag aaggcatacg tagaattgaa gatattcccg
    37321 cacttaactg gaaggacagg ctgttgaaac atggtattta taatgtactt gcttatttca
    37381 actggtcaat ttatgccctt tttaattttg tgtatccaaa caaatttcac cctacagact
    37441 ttaaatataa aacaaagtgg gcagctgctg actcattgga taacgcattc cactatatgc
    37501 acaaaacctt taaaaagaaa aaatatacgc tggatgaaat tttgctattg aacacatcga
    37561 attttagagg tctgggactt gcaggaatgc tggcatctgt ttttgactca tcaacactaa
    37621 gagctaaaga gtactacctt tataaaacta tcggtgacaa agcacacata aaagagttaa
    37681 aagaagatat cagaaaattg aaaaaggaaa actacgataa aaatatcagg aaaaaactta
    37741 atgaagtttc ttcagtaggt ggttttatat ataatctgca tgataatacc actttgtata
    37801 attatataaa acgccatgct aaaaaaaata atatgagtat aaatgatttt atatccagtt
    37861 acggctttac atataagagt gccagaaagg atattaaaaa catcagtaag gaagatatat
    37921 ggaatcttcg caaacaaggt cttacttatg ttcagatagc ccaagagctt gaaagcaatc
    37981 ctacaacaat aacggagatg tgctccaggt attttggggg agatcccctt attccaagac
    38041 caatagagga ttatgttacc gttcaggaat taatgaataa atatcgtgta gatcacaaga
    38101 cagtaatgag aatcgtttat gaaaacaatt ttgaaaatca tacaactatt cgtttcaggt
    38161 acttaaaaaa gtcagagata gaacctgcac tgcaggaata tgtagaaaca agcaagcatc
    38221 atagattcat ggtaaagaga tatgctaatt gaactgccgg ataaacgcag gtaaacgggt
    38281 cagcccccgt ttacctggta taaggtgaac acattgattt tgaatgcacc ctctggcatg
    38341 tctctacctc gttattaaag ccattacttc agctctggtt tttgcttctg ttcttactat
    38401 accccttagt gcagatgtaa ctgtttttga gcctggcttt tttacacccc tcatagtcat
    38461 gcataaatgc tctgcttcaa ctactacagc tacaccgaaa ggttttatgg attccataat
    38521 tacatctgca atttcgctgg taagacgttc ctgaagctgc ggacgttttg caattgtgtc
    38581 aactattctg gcaagtttgc ttaatccaac aattttcccc ttatttggta tatatactac
    38641 atgggctacg ccaataaatg ggagtaaatg gtgctcacaa acggaataca gcggaatatc
    38701 cttaaccatt accatttcct catgtccatc ttcctggaat attttaatta cattcttagg
    38761 atcttgacct acccctgcaa atatttcctc acacattcgc gcaacacgat cgggtgtttc
    38821 tttcagccct tctctgtcag gatcttctcc aatagcagca aggaactccc ttaccgcacc
    38881 tttaacccgt tctttatcta tcatattatt aattctgcct ttcttgtaca tggatctcag
    38941 gcttttatat tattaatcca ttgcataata atttatattt atgattataa tcttttttgg
    39001 ctgaaagtca atttgttggc tttaaaaagg tgcattttct atatttgaag aaaaatatgg
    39061 caagaaacga ttttcatgta taatattcac tgttcaaatt ttatactcgg aagatcgtat
    39121 actgccatat tttgtatcat aggaagaaaa tgcgcccgca gtttagctgc tttttacatt
    39181 cccaggtaat gtcggacata attcataatc actccgctga atatcatacc gattcccata
    39241 aaaacaatac ttccaacaat taacggaagc cctgcccggc tttgattttt tactgccagt
    39301 attcccatta ttgctcctac aacgccgaat atgaaggggt aggctaaaag tgataatatt
    39361 gcggaaatcc agccaatgac cagaaacaaa gtactatcac gtgcttcagc tacttcttcc
    39421 ctgttttcag tattattaaa ttcatccata aatatcaccc cagtttatta tgctactgaa
    39481 gaatatacag tatgcataaa agccaatttt tttatagtga acctataatt aattttgctg
    39541 caaaaactgg agtattgaag cagaattaag catttgaagc taatgtaatt ttgcgtttag
    39601 tatattcaat aagaccgtca gcatttataa tttcctgcat aaactccgga aaaggagcag
    39661 ccttgtattc atctccgctt gttaagttaa caattttccc tgacgacaat tccacagcta
    39721 caatgtctcc tttgttaatt gcttttgctg cttcagggca ttccaaaatc ggcagaccta
    39781 tatttattgc attcctatag aatattcttg caaaggtttc ggctataaca catgaaatac
    39841 ctgaagcttt aatagctatg ggagcatgtt ctctggatga accgcatcca aagtttttac
    39901 ctccaaccat tacatccccc ttttctacat gggatataaa tgttttgtcc aaatcttcca
    39961 tacaatgttt tgcaagttca gaaggttcgt gggtatttaa gtaccttgcc ggtattatta
    40021 catctgtatc aatgttattt ccatacttta tggctttacc ttgtatcatt aatttttcta
    40081 cctcccagca ccatatactt ttagttaatt tataattcat ccggtgaggc tatccttcct
    40141 gcaatggcag atgcagctgc tattgcaggg cttgcaagat aaacttcact tcctgtatgt
    40201 cccattcttc caacaaagtt tctattggtt gtggataccg cccgttcacc ttctgccaga
    40261 atacccatgt atcccccaag gcagggacca catgttggag tacttacagc ggcgcctgca
    40321 tcaacaaata tatcaaagag cccttcattc atcgcctgct tccagatttt ctgagttgca
    40381 ggaataatta tgcaccttac atcagggtgc accttttttc cttccataac ttcagctgca
    40441 actctcaagt cttctaaacg cccatttgtg cag
//
LOCUS       DAOSDD010000007        38669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_562_length_38669_cov_5.333143, whole genome shotgun sequence.
ACCESSION   DAOSDD010000007 DAOSDD010000000
VERSION     DAOSDD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 38669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38669
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_38669_cov_5.333143"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            804..1097
                     /locus_tag="PK733_01225"
     CDS             804..1097
                     /locus_tag="PK733_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09200.1"
                     /translation="MEKRNFERNNRRRKIVQLHEKDPLLKSLNIEQLINIISPEKIKK
                     TQVILKEYLEGFKYSHIEKKIVLAYLIGELQGLDDFYSENAGKSIAEKLKVIY"
     gene            complement(1243..1581)
                     /locus_tag="PK733_01230"
     CDS             complement(1243..1581)
                     /locus_tag="PK733_01230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOJ09201.1"
                     /translation="MAYIPQQGDIIYLDFDPQSGHEQKGRRPAIVVSNNAFNALSRGA
                     LVCPITNTNRGIPLHVQLDSRTKTTGVIMCDQVKALDMQSRSAAFIEKAPEDLIAEAV
                     DILIAMVEIL"
     gene            complement(1581..1823)
                     /locus_tag="PK733_01235"
     CDS             complement(1581..1823)
                     /locus_tag="PK733_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ09202.1"
                     /translation="MYTTIQKWGNSQGIRLPKQLLEALDLRESDRVEIGRQQDAIIIR
                     KAGMRHRTLEERLVGFKGPYEFSEWDTGAPTGNEVL"
     gene            complement(2624..2809)
                     /locus_tag="PK733_01240"
     CDS             complement(2624..2809)
                     /locus_tag="PK733_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09203.1"
                     /translation="MQGKFEVIGHKEILSFVQERRPHLVSFDGQRAYTSRPEALAEYV
                     YKIKGSFNKYRLLFIPA"
     gene            complement(3409..3483)
                     /locus_tag="PK733_01245"
     tRNA            complement(3409..3483)
                     /locus_tag="PK733_01245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3448..3450),aa:Glu,seq:ctc)
     gene            3981..4346
                     /locus_tag="PK733_01250"
     CDS             3981..4346
                     /locus_tag="PK733_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOJ09204.1"
                     /translation="MARPRKWRKVCCLPKSNLFGPLNAQIVSADTVLMTVEEYETIRL
                     IDLEGLMQEECAERMGVARTTVQRIYYDARKKLAQSLVSGSVLKIEGGDYKLCDRSEQ
                     AYSCGRCRRHRHGNNVVYS"
     gene            4387..5250
                     /locus_tag="PK733_01255"
     CDS             4387..5250
                     /locus_tag="PK733_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOJ09205.1"
                     /translation="MSEKCNQNCSACGEDCSERKEKPVDFSAKPHELSNIKKVIGVVS
                     GKGGVGKSLVTSMLGVLMNRKGYKTAILDADITGPSIPKAFGVKEKVTGNELGIYPVK
                     SKTGINIMSVNLLLENDTDPVVWRGPIIANTVKQFWTDVIWGDVDFMFIDMPPGTGDV
                     PLTVFQSIAVDGIIIVASPQELVSMIVSKAAKMAQMMNIPIIALVENMAYLNCPDCGK
                     EIKVFGDSHIEEIAKKYNIDKLARLPIEPKVAAACDKGLIELFSGNWLDGIVEILENM
                     EENKNEDCSRK"
     gene            5228..5584
                     /locus_tag="PK733_01260"
     CDS             5228..5584
                     /locus_tag="PK733_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ09206.1"
                     /translation="MKIAVASENEMVAEHFGHCSNFNVFEVENNCVIKNQSIPNPGHK
                     PGFLPNFLNDIGVNVIISGGMGGGAIEIFNEKGIEVITGIRGKAEAAVNSYLQGTLKS
                     SGSVCHEHQHHDECGN"
     gene            5672..5935
                     /locus_tag="PK733_01265"
     CDS             5672..5935
                     /locus_tag="PK733_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09207.1"
                     /translation="MKVYVRLNGNSSGNMMDPRFGRAAFFIFVDIDSLMKSQDNEQKQ
                     SLRILKRNGPIMHKRFLSEWIPLENRYFKEMKIQEQSDLVFSG"
     gene            complement(5980..7056)
                     /gene="amrB"
                     /locus_tag="PK733_01270"
     CDS             complement(5980..7056)
                     /gene="amrB"
                     /locus_tag="PK733_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOJ09208.1"
                     /translation="MKHLKKHYKIFIIMIIGLCMVLLSGSYYSIAISSATVYKIFGQW
                     KDTLLLRLPSMSPNIQSSNIQSGGEGMPGASFNQQRQVVNNLQVNEKRLQCRYYNEKD
                     FKQSVNRVQLDDYGHLAETEIIESSIKGGIVPHHLLASNMIASFFKTISVEQPEVVII
                     IAPNHKGTGVKAVHTGSWSWQTPFGILEADVDIVNSLVDSKTADMNFDLLEEDHSIAG
                     LVPYIKYYMPDTKIVPIMLHGSFGLQNAQKLGQNLQEKLENENKKSTIIASVDFSHYL
                     PLEEARQMDEISIKAIENRDINIIQHFNNDYMDSPPSIIALLSAMNASGAEHMKMLDH
                     SNSDEIAGARSSETTSYFTVVFFR"
     gene            complement(7053..12371)
                     /locus_tag="PK733_01275"
     CDS             complement(7053..12371)
                     /locus_tag="PK733_01275"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOJ09209.1"
                     /translation="MNNKELLKNKKLIISVSVITAISILIISLLVIPNLLSDRAPASF
                     NPGDSYTSGSSSSLKIDGAIDIVPMTADSTGVDINSCFKIVCENKQSRSFFQKTLSIS
                     PQQNYEIKEISDKEFQINFSDALKPNSIYKMSLTGVSNNKDKSNDNTSNTFDYNYSWA
                     FQTKKSFALVRTLPGDRSTYVPVDSGIELNFSHEGVENIEQYFEINPKVNGRFEHHNK
                     TAVFIPEKLDYGTIYTITVKKGLGIKGSDEVLSEDCVFAFETRKSPEQENTEYLSFTD
                     ILYNFTPQAEPVLSVYASQDFKDQEIAVEVYKYSNTDDLLEHLKLYNTPAYQAFGRSD
                     SLKFDTTNLQRVTEFNTKVMNNNDIYWYGSFIIFPSNLPEGHYLINVKSKTTTYQTHI
                     QVNDMAVYAMAGTQEGLVWINSSSTGQPIEGAAVSTVSLPSSSSPSVSSSSAQSNSLS
                     SDVIQTVKTDKDGVAIIPGNILEICNKLKDLKRQKDQRLFFSISKNNSSDSPVFLAPL
                     TYYSWQYSNYDYPGRKSSQKYWAYLYTDRDLYLPSDAIQIWGMVRPRIKGNLPSKGIL
                     RLHRADYFYGTGYAYGENSSLSEIISKEIDISSLGTFKSSMEISNLNPGSYFIELKLG
                     DESVIQKYFSIRQYTKPAYKVDITPDKKVMFEWEKLNVDIQASFFEGSPVSGLDLKYS
                     HYTTWDTRKEGNLICDENGKAELEILPISINNSWHPIALSLNLNNAKAEEEEIHSYSN
                     VIVFPRDIMIEVNASSKNQAGTTGKTEDKSGMAGGQTGLIEIKTNRINLEKLRSQSDS
                     WYMNTDEFRGDLVNIPLNIKIYEQHWGREETGEYYDFINKKVRKTYRYFNVKNLLQEF
                     KANTLNSEYSYEFPIKENENYVIEVEGADSRQKPIVETAHLYRYAHPQDGNIDRYTIS
                     TGYHNDMEFGRFKSGDTVALNIKKNNEYLSSTPGSRFLYMILKDGIVSHIVENDPDYS
                     FSFKKDFIPNIYVKAVYFDGKNIFDVENTLIGYDYKEKELIIDVKTDKHEYRPGDTVQ
                     LEVDVKDVQGTPCISEVNLSVVDEAFFAVYGQQVNTLASLYQYCFNSGILTEYLSYKP
                     ININFGAPEMGGEGGDESTRFIFKDNAFFDTITTDNNGKGKASFKLPDNLTSWRITYQ
                     GIAYQKNSDDLKAGSGKINIPVKLPFFVDVIFNNVFMDGDTVAISARSFGTQLESDKK
                     VEYTVVLEKEGIENNNVSSGTISDANKTLEKKTYQSEGKGNDLTNIDLGKLEEGSYSV
                     TVKAKHGNYSDAIKRDFKVVKSLLEGSKLEHYKLTDSLQPEGGKSLTTLLFNNEATST
                     YYNTLLSLLYSWGERVDQKLSRKIARELMSKYYKDDIWWAEGTEDYQLNSYQQYDGGI
                     ALLTYDSSNPELTAKISSLSKDLLLKNSASGNSASISSLNSLSNSLFDNTSMKWYFYK
                     TLENRNVTPEDAAASLWGLAALNEPVLIDIENLLKSDDIGIKEKLYLSIGLAEFGNVK
                     EARNVYDDIIKQYGVTGSPYVYIDSKAGRDDTIELTSLCSVLGFKINAPEKAGLLKYV
                     QDNSTKYILTNIENLICVAHMTPDTAQNGSFTVDVNGRKENITLDKNRVFRLVLTPEA
                     LQNIKFSNVVGDIAVTSSYTAPVKDFAKDANSQVKIKRSYSLDGIVQSSFNQSELVKI
                     TLEPEFLETAPDGYYEITDILPAGLRYVSATGHFDQKWYPVERNGQKIVFGFYYNKNS
                     KYRERSITYYARAVTPGRFTADHAFIKHSMSDISGFADKVQVEIKK"
     gene            12789..13553
                     /locus_tag="PK733_01280"
     CDS             12789..13553
                     /locus_tag="PK733_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOJ09210.1"
                     /translation="MSENINSYKVITATELKNNLGKYLDYVMEDKEVVVTKNGTKAVR
                     LTPYINDYDRYFVLKEKALDYLYGGLKVSYEEFMEIYEKSDLRMEFINGEIVLLSSPS
                     TRHQEISGDLHVIFKEYLKDKKCKVFYAPFDVHFFKVGFKEPDVMQPDLLIACDTDEK
                     VNEKGRYMGTPALVVEILSSSTRSKDMVDKLNTYMISGVKEFWVVDPQNESILIYSFK
                     DYEIDDFVAYANKKEDVARSFNFEGLEVKCSEIFGL"
     gene            13851..14471
                     /locus_tag="PK733_01285"
     CDS             13851..14471
                     /locus_tag="PK733_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOJ09211.1"
                     /translation="MPYFPSNIENVPMHICIFFAYDYAPKEISYEEFIERYEKTEERL
                     EYIDSRVYAMGSPNHTHQSIVGYLFNSFYTFFNGKECKPFVAPYDIHFENSHNKACVQ
                     PDIFVMCDKENLRDGKYYGVPALVVEVLSPASCTKDTMTKLNLYWREGVSEYLLVEPR
                     EQIVYYWYFREKELIIQKTLGVEHVLKSNMFSGLEIKISEIFQSIY"
     gene            14500..16137
                     /locus_tag="PK733_01290"
     CDS             14500..16137
                     /locus_tag="PK733_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HOJ09212.1"
                     /translation="MEQKNESGQKDFIILDKKDNVAVALKDMDIGDIISVAGDAAMDI
                     SDTIEDINDTTVCDGKAERIKAGHKVAIRDIPKGENIIKYGYPIGHATCHISAGQWVH
                     THNARTNLHGILEYTYKCNTDCNIDCKTLVNSGDTGDKAFSSPDDNKANPFTFKGYLR
                     EDGNVGTRNEIWIINTVGCVNKTAEILAREANRIFSNKIGQNFDGIFAFPHPYGCSQL
                     GEDHFTTQKLLSALAKHPNAAGILVLGLGCENNNIDEFKKILDEKNKDRIRFLSTQDC
                     EDEVSSGIKLIAELIDYTAPFQRQNCPISKLTIGFKCGGSDAFSGITANPLCGEFSDI
                     ILEQGGTTILTEVPEMFGAETILMDRCVNKDVFNKTVEMINGFKEYFIRHNQTIYENP
                     SPGNKEGGISTLEEKSLGCILKGGSGAVADVLKYADKVKKTGLNLLEGPGNDAVSITA
                     LTAAGANLIIFTTGRGTPLGAPVPTIKVSTTTDLYNRKGAWIDYNAGQLLECKSIKSV
                     ANDFFSFIISVASGEIKTKNEINCYREIAIFKDGITL"
     gene            16317..16640
                     /locus_tag="PK733_01295"
                     /pseudo
     CDS             16317..16640
                     /locus_tag="PK733_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            16792..17448
                     /locus_tag="PK733_01300"
     CDS             16792..17448
                     /locus_tag="PK733_01300"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HOJ09213.1"
                     /translation="MINKIRAYIDHAFEGIPQTKKVLELKEELVGNLIEKYNDYLKNG
                     KAEDEAYTAAISGIGDISELVESMRDVDPFAQPSTKERSRHALFISIAVAIYIISVFM
                     VPIYTINFGKPVTGIFIMFLFIAIATGLIIYSNMTYKNYIKMDDSLVEDFKAYRASSN
                     KQRAAFNSFKSAFWSITVAVYFLISFIYGIWGYSWVIFIIAGAIEKIVAGIIQLRGEE
                     "
     gene            17445..18377
                     /locus_tag="PK733_01305"
     CDS             17445..18377
                     /locus_tag="PK733_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOJ09214.1"
                     /translation="MMDKRLIKGNIILWSVVLVIAIAVFVAFARGNYCNPFGFPFNMK
                     TANRSQFVVVKEQSIKNDTHNINDVTVDWISGNVDIFKSENNEIKIVQLAGEKFSDND
                     LFSYKIRGGKLSVIDERKGKTSIGFNFNNFNNGSDLEIYLPEKEFNSIRINTVSSDIT
                     SESLNTEILTFSTTSGDVILSGKFSEVDVKTVSGDIRAENLDVQRIALDTTSGDISLT
                     GSLTELDINTISGDAKVYSSDMIKNVKSNAVSGNVTIAIPENDGFTIDFSKVSGNLKS
                     DFALVTNGNRHSYKDGTARFSANSVSGNFTIFKR"
     gene            18605..18820
                     /locus_tag="PK733_01310"
     CDS             18605..18820
                     /locus_tag="PK733_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOJ09215.1"
                     /translation="MTNKTYQLQTLTCPSCMAKIQGALRKTAGVKEAEVLFNTSRAKV
                     SFNEDQVSSDTIKKTIESLGFKILGEK"
     gene            19013..20878
                     /locus_tag="PK733_01315"
     CDS             19013..20878
                     /locus_tag="PK733_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOJ09216.1"
                     /translation="MIKLTKGKRVLISGILVAVSFILKNTIGHQLITSIVMIAAAIVA
                     GTPIIRNAISALRYRILGIDALVSIAVIGAMFIGEYWEAAAVTFLFMFGEYLESKTIE
                     KTRSSIKSLLDLAPDTARVIRDGKEIEVSPEKLVKGDMAVIKPGEKIPVDGIVTEGTA
                     YVNQAAITGESIPVAKNIEDSVFSGTIIESGYLIVRADKVGGDTTFARILQMVEEAQD
                     KKARTQKFLEKFSRYYTPSIIVLSALLFIFTRDLVLSLTLLVIACPGALVISTPVSIV
                     AGIGNGAKHGVLVKGGEIMEKLGRVKVLAFDKTGTLTIGKPVVNHIKAYGINKHELLR
                     IAAIGEAYSEHPLGKAIINKAEKDLGTISQVPEESEIIAGQGLKVSIDKSIILIGNRK
                     LLSENGVQLDDNHEKYISSEEEHGQTAVIVANLERVLEIISIADKVREDAKKLIANLK
                     RLGVKKIVMLTGDNKRAAKAISNELGINDYYAELLPDGKVKTLKELKEKYGLAAMVGD
                     GVNDAPALASADLGIAIGGAGKDIAMETADIVMMSDEIRKLSFAVGLSRATVRNMKQN
                     IIFALVVAGTLLTGVLIKTVDLAFGMLIHELSVLLVIINAVRLLGYGKKQKSGYA"
     gene            20895..21575
                     /locus_tag="PK733_01320"
     CDS             20895..21575
                     /locus_tag="PK733_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ09217.1"
                     /translation="MNCNCHKREGRNCIEIVPIFSSLNHEEMMEVAGITSDRTYEKGE
                     MIYIAGDKGEKLYVIHKGKVKISRVSESGKEQVIRVLGPGEFMGELSLFSSSPLTDNG
                     EALEKTTVCIISKDKIKELMAKYPVISFKVMEELSRRLEKVENLVENISLHGVEKRLA
                     DTLISMVNDKNEVILKMSKKDFASHIGMSQETLSRKLSAFQEMGLIKLIGHRRIIILD
                     KEALSEIE"
     gene            21751..22470
                     /locus_tag="PK733_01325"
     CDS             21751..22470
                     /locus_tag="PK733_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ09218.1"
                     /translation="MCKNDETERPSVLIVDDDKDIVKAIAINLENDGYNVFKAYDGLQ
                     ALDFINSQSIQLILLDIMMPKLDGLSATMKIRQEKNIPIIILSAKSEDTDKILGLSMG
                     ADDYVTKPFNPLELMARVKSQLRRYMTLGDIEANGKTNILKSGGLCFDPDTRIVTVDG
                     EPVRLTATEMKIVELLLRSPGRIFPAEEIYERVWEEPAYSVENTVMVHIRRIREKIEI
                     NPKDPKYLKVVWGIGYKIEKI"
     gene            22445..24523
                     /locus_tag="PK733_01330"
     CDS             22445..24523
                     /locus_tag="PK733_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ09219.1"
                     /translation="MDIKSKKSKGMIIWLCFFIGVNIVGFIASGSIVEIINSGYTDTI
                     DSIRDVYKTDIKDTRQFKHTITLMFNMLAKSLTDDNYSSMIEAMGEDLNKEEENLIFY
                     AENFKTGKRIANSKTDLGISVKGFPSLLQGYDYYFYYNGEEFFAGNSDKPQNIDKKDI
                     GYSNLYHWGCFSENINSKFPGIRDCRILLVVKKDITKVISETSRLYNLKKRFQVMKWT
                     IIGIIVVFLFGVALLIISILNRKLKHEFDFRLAKLSGKLWFEPKALVSLFVIAVIMEA
                     ERNTSKLMYSTQLRFAFFLIIAGWWFYLMFIDLVTNKRRFFSNNSINMIIRKYRSFEG
                     KKPFQKGMILRLFMFIGAEAILVFLAGAFCIIIIQRMDSFFFYCIFIVAAIGVYLIYR
                     YQRRYSNTITDIGRLVDQIEAIKAGGVTDELVLDPDTDMYNAAKNLNSIKEGMNKVIE
                     EKVKSERTKVELITNVSHDLKTPLTSIISYVDLLSKEQGLPEHVNDYVKILIQKSNRL
                     KTLIEDLFDLTRASSGEIKVDYERLDLGKLIKQTMADLNEEISRSELAFRVNIPEQPV
                     FILSDGKKLYRVFLNLISNALKYSLPGSRVYIDLIVQNMNNKTNGETKEIDYEVVVII
                     KNTANYEMSFSQEEILERFVRGDKARSTEGSGLGLAIAKNFTQVCGGKFDIVLDGDLF
                     KVILSFAAAK"
     gene            24883..26544
                     /locus_tag="PK733_01335"
     CDS             24883..26544
                     /locus_tag="PK733_01335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOJ09220.1"
                     /translation="MKLPDEFLTKMQEILNKDEYKAFLMSYNMPRYYGLRVNTLKISV
                     ERFMEISPFKLERIPWTKDGFYYNEGENPGKHPFYYAGLYYIQEPSAMLPATVLDAKP
                     GEYILDLCAAPGGKTVQIAAGMKGEGLLMANDTSAERLKALVKNIELCGVRNAIVTNE
                     QPQKLAAKFPGYFDKILVDAPCSGEGMFRKDENAIKSWGKYKCDNCAVIQKDILEYAD
                     VMLKPGGRLVYSTCTFSPEEDEMIIGNFLKKHNDYDVLKIPLTAGIERGKPEWGGGDV
                     RLAGTVRLWPHKVKGEGHFTAVLEKTAAFDGHINDKQTQNQEDNFSDNSFSKNIYIKD
                     INYSRNINHSKDINSGIDTLPKEYAGYFKEFMKDNLNIEIDGNFMLIGNNLYYFPVPL
                     PDLSGIKVVKFGWYLGEFINVQALGRPVSNEQTTIKKQSPHKQNANKQKSKEQYLRGQ
                     YSKEQNLMNQISKNLVLKRRELEGQFEPSHPLLISLKMDDVKNTSNFTADSSEILKYL
                     KGETLITDGQKGLTAVCVDGFTIGWAKQTGGMLKNLYPKGWRKHR"
     gene            26988..27905
                     /locus_tag="PK733_01340"
     CDS             26988..27905
                     /locus_tag="PK733_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ09221.1"
                     /translation="MGVYLIKRISLAVVTLFLVATLTFFLMFLVPGGPFLAEKAPTAQ
                     TLAALEKKYGLDQPVSAQYINYMKRLIRGDFGPSLKLRGREVNEVISTGFPISASIGG
                     IAVILAVLVGVPLGTFSAVNRGKWLDNIVLFISTLGIAIPGFVLATILMYYFGVTLRI
                     LPTYGLSSPKHFILPVISLAFYPTAYISRLMRSSMLDVLGQDYMRTARAKGLSQFKSL
                     FKHALRNAILPVVTYLGPLLAYILTGSFVVEKIFTIPGLGGQFISSITNRDYPMIMGT
                     TIFLAFLIILMNMVVDIVYKIVDPRIKLK"
     gene            28047..29108
                     /locus_tag="PK733_01345"
     CDS             28047..29108
                     /locus_tag="PK733_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ09222.1"
                     /translation="MTQKIGDKMNPLSLHIDVSDFLPAEESEKQSMVVMRESVNFWKD
                     GFRRLRKNKLAMISLTVIIVIMIFAFIVPVFYPYSYEQQIRGSENLNLMEYSKEELAR
                     KEEGEFVFPHILGTDSLGRDYAVRIMMGSRISLLVGIIASAIILLIGSIYGSISGYFG
                     GKVDLIMMRIVDIIYTVPDILIIILLSVTLKYPLKDLAENVPGFGWINTVGVGLISIF
                     IVFSLLYWVGMARIVRGQILTLKEQDFITAAKALGASNSRIIRKHLLTNCIGTLIVTT
                     TLQIPSSIFTESFLSFLGLGVNAPMPSLGSLASQALGGIKSYPHRLLLPAITISLIIL
                     SFNLLGDGLRDAFDPKLKN"
     gene            29121..30125
                     /locus_tag="PK733_01350"
     CDS             29121..30125
                     /locus_tag="PK733_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09223.1"
                     /translation="MSNILLDIKNLKVSFFTPAGEVKAINDVSIQMEEGDVLGIVGES
                     GSGKSVTSYSIMGLVPFPGRIIDGTIDFNGHRINDMTEAELRSIRGNEVSIIFQDPMT
                     SLNPVYTIGNQIREAILLHTNLNKQQAYERSVELLKLVGINEPEKRLKQYPHELSGGM
                     RQRVMIAIALACEPKLLIADEPTTALDVTIQAQIMELMTELKNKINMSIILITHDLGI
                     VSSVCRKIMVMYAGKIVESGTIDDIFYNPKHEYTKGLLRSIPKLNTKEHEKLVPIEGT
                     PVDMLNPPAGCPFAPRCEKCMKICLREMPEFTKISDIHYCACWLLQKEAFEASAGGNK
                     "
     gene            30125..31138
                     /locus_tag="PK733_01355"
     CDS             30125..31138
                     /locus_tag="PK733_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ09224.1"
                     /translation="MGKSINQKHKFATLREGALVEIQNLKQYFPIQDGLFGKKYVKAV
                     DDVSFYIDKGETLGLVGESGCGKTTAGRTILRLYEPTGGRIIYNGTDITYVDMMPYRR
                     KMQIVFQDPYASLNPRMTVGDIVGEPIDIHKLAASNKERSEKIIQMLDRVGLNSEHAN
                     RYPHEFSGGQRQRIGIARALAVDPEFIVCDEPISALDVSIQAQIVNMFEKLQEEMGLT
                     YLFIAHDLSVVKHISNRIGVMYLGKLVELADSYELVFHSVHPYTRSLISAIPVPDPNT
                     SRTQKRIILEGDVPSPLNPPSGCRFRTRCPYADKICAEKEPEFREIGNRHFAACHHLD
                     KVH"
     gene            31280..32872
                     /locus_tag="PK733_01360"
     CDS             31280..32872
                     /locus_tag="PK733_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ09225.1"
                     /translation="MKKIALLLVMVMVFGTFLSACGNQKIASTPELVVCVGPEPDTID
                     PALNSAVDGATLLIHGFEGLMTLDKNGKPIAGQAKSCTPSDDKLTYTFKLRDNLKWSD
                     GEPVVANDFVYAWNRAINPETASDYEYMFESIEGYDKGALNVTAPDDKTLIVKLVSAT
                     PYFLELCAFPTFFPVRKDIVEANPDKWALDPATYVGNGPYMMTEWVHDSHMIYKKNPN
                     YWNVSKLGPDSIKFILMDDDNAIIAAFENEEILLADSMPNDEIDAWRDKKEFNIEGQL
                     GTYYISFNVKKPPLDNEKVRKALILAVDREYITKNIGKAGQEPAGAYVSIGLSDADLK
                     KEFREVGGDYYDPSGAAYEANLAEAKKLLADAGYPDGKGLPTIEYLYNTGTGHQLIGE
                     ALQDMWKKIGVNIELVSQEWKTFLNTRKNGEYFIARNGWLGDYNDPISFLDMWITGGG
                     NNDAQWSNAEYDALISKVKSSSDRAERMKLMHQAEDIIFKDSMLCPIYYYVDIYLKNE
                     KLDGFYSSPLGYKYFMYSSVKQ"
     gene            33236..34045
                     /locus_tag="PK733_01365"
     CDS             33236..34045
                     /locus_tag="PK733_01365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ09226.1"
                     /translation="MAGTQRLDKILSNFGYGTRREVKQLVKQGMVKVDDIPAVDSAMH
                     VNPETSAIEVNGKKLNYREFIYIMMNKPAGVISATFDNKLKTVVDLLPDEYKVFNPFP
                     VGRLDIDTEGLLILTNDGPLAHMLLSPKKSVPKTYYALVDGRVTEEDIKAFNNGVVLD
                     DGYKTLPAQLKILKQGEQGAHSEIEVIIYEGKFHQVKRMFETVGKKVKYLRRMAMGSL
                     VLDENLETGQFRELTEEENKSLSMVLQATTAIVSGAAIDIVPEAAADREDD"
     gene            34038..34184
                     /locus_tag="PK733_01370"
     CDS             34038..34184
                     /locus_tag="PK733_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09227.1"
                     /translation="MIKNCHREFMIKAEKVSRLDKNSMKQYRELKDELNIEPALERLI
                     RLDL"
     gene            complement(34224..34931)
                     /locus_tag="PK733_01375"
     CDS             complement(34224..34931)
                     /locus_tag="PK733_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09228.1"
                     /translation="MESYVTSHPIPNREGILILKVPESDIGLPHGFMYLRLIDLRGFE
                     EAMIDFAEECDELQILIDKVCDYNVRQMKLICEKSDSPIIHVGDDLGMQNGIAIGAAK
                     WRNYLKPAFKRIYDVSHQFKKYVYMHTDGDIIEIMPDLVEAGVNIINPQYSANGIDRL
                     VRTCKGKIPIMLDLDRQFFPFGTPQDMRDHIRETIEKMYLPKGGLGINIEIGFDVPIE
                     NIEAIVDEINRMRFYKG"
     gene            complement(35028..35186)
                     /locus_tag="PK733_01380"
     CDS             complement(35028..35186)
                     /locus_tag="PK733_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09229.1"
                     /translation="MQNTTDLIIKAITYQYPEEIPVYFNFLPATMKKSGNEIKKITAK
                     YEDFFGDH"
     gene            35300..36136
                     /locus_tag="PK733_01385"
     CDS             35300..36136
                     /locus_tag="PK733_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09230.1"
                     /translation="MLLSKYRQVLCEGTLNLPGLTLFGHNVTTKAVLPMGTHIHDGCI
                     EIVVVVKGNESYYVENRKFELSGGDVFVAYTDQPHRSGNTSQGVCEIIWFQIKPAVQE
                     NFLGLSAPFGDLIRSKLMAIDTHILKIDREGLFYLRKSFENFLRLREENRIYGQSLLI
                     SFLSRLLFTQTQNRKDDELIENIHKYIEENIYDVIKTEDICSKFCISLSGFKHKFKEY
                     TGVTPRDYINDRKIQKAKELLQSRKSVTETAMQLSFNSSDYFSVVFKKYTAFSPTQFR
                     KL"
     gene            complement(36190..37119)
                     /locus_tag="PK733_01390"
     CDS             complement(36190..37119)
                     /locus_tag="PK733_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ09231.1"
                     /translation="MAKKILTVEEKLKKIKKRFAILLSFFIAFIAGTGFYIYLNYDYL
                     VFKHFISHHYIYTDALETLYKNELKRDVSGRYFQYFDDLVISAVTEKIRKTNKDRYTY
                     LYIPEQYKKYKEEEKEEASLSELKVLNENTVYIHITNFSTYTQKFLQNNVSEINKYPR
                     LIIDLRSNYGGDTLAMNKMSDLFIPKGSVISVDKMRLFNKTYKAKKEKVIKNETIIIL
                     QDKNTASASENFIAALKDNLDNVTLIGDKTFGKGIGQFTIPLKKGFAVKATTMLWYTP
                     KGINIQGKGIEPDIYYMDDDIIDFALDKLPDLN"
     gene            complement(37327..37560)
                     /locus_tag="PK733_01395"
     CDS             complement(37327..37560)
                     /locus_tag="PK733_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOJ09232.1"
                     /translation="MTVKVYSTPACPWCSVAKKYLSSKNVAFEDYDVSADRQAAIEMV
                     QKSGQRGVPVIEIDGNIIVGFNQDAIDKLITKP"
     gene            complement(37861..>38669)
                     /locus_tag="PK733_01400"
     CDS             complement(37861..>38669)
                     /locus_tag="PK733_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ09233.1"
                     /translation="AIKISNLLNKFGINITRKLFAPVYNNFGGNIRLFISGAAGIDPH
                     IAKGFRDFGLIFVQGYGLTECSPIAALNRDVYCKDDAAGLPLPGLEVKIDLPNEEGVG
                     EIKVKGENVMLGYYGDSEATEKVLKSGWFYTGDLGFIDSDGFVHITGRKKHVIVTKNG
                     KNIYPEEIETLLNKSPYIMESMVYGKDDSEFGDVVVSAAVVPDMEKIQEDFAGKTLSQ
                     EEIHELIRKEVKQVNKKLVTYKYVKDFHLRENEFAKTTTKKIKRYMEKVE"
BASE COUNT        12430 a         6554 c         7668 g        12017 t
ORIGIN      
        1 aaataaaggc atttatggcc aggtataagt aagtttgtta aggttatgag cggattatat
       61 cggttatttg taattatatc gtccagatat agttatattg tttaagttat aattatattg
      121 ttcacatgtg atcacatcta ttatatgtga tctaaacaga aaacccggac ttgtatatag
      181 acaaatccgg gttttctatt attattctgg tgagccactg tcagtaaaat ccgaacaatc
      241 ttactgtagc tcgtctgcgt tttccagcat ctcaatatat gaaatctgct gaccatcttt
      301 gacattataa tatatgatta gcttgttata aaacagctaa aagatactat ttattagaac
      361 cagtagacgt ccgaaacgaa atcgggaata gaacattcat ccgagagtta gcttgatttt
      421 gaagaggatc cctcagctca agtagcattt aagcgcattg aggattttga tttcgtgcgt
      481 tcggtgagag aacaagttga gaagcttccg gatccggagc gaaaaacaat aaaaaagttt
      541 cactttaaag agataataga tatgtcgcag ggaatagcga ccagtacgtc cctggcaaga
      601 atgaccgaat ctgatatgtt ggggctctca gcagctgtta ccgccctcag catcgaatcg
      661 caagctggct ctacgtcagg atagaatatc ttgccatagg tattttatcc tcccctcctt
      721 ctatagggtg taggcatgcc tatttagctg gcatttcgga ctggtatgcc tgcatctact
      781 tttaaaattg gagatgatat cacatggaaa agagaaactt tgaacgtaat aacaggagaa
      841 gaaagattgt tcagctgcat gagaaagatc cgctccttaa atccctgaac attgagcagc
      901 tgattaacat aatatcaccg gaaaaaataa aaaagaccca ggtaattcta aaagaatacc
      961 ttgagggctt caagtattca catatcgaaa aaaagattgt gcttgcatac ctcataggtg
     1021 agttgcaggg acttgatgat ttttacagtg aaaatgcagg gaaaagcata gcagagaagc
     1081 tcaaagtgat atattagtca ggtttcaact ggggctatat gctggcatcg ttgtattacc
     1141 ctcaaggggg gaagaaagat gaacagtgag gatccagttt cggatataat aaaaaagccc
     1201 gctgatgatt gttatgtcac caatgggctc tgtttttatg ctttataata tttcaaccat
     1261 ggcgatgagt atgtccacgg cctcagctat cagatcctca ggtgcctttt cgataaaggc
     1321 tgcacttctg ctctgcatat cgagggcttt cacctgatcg cacatgataa cgccggttgt
     1381 ctttgtccgg gaatccaact ggacatgaag cggtatgcct cgattggtat ttgtaatcgg
     1441 gcagaccagc gcgccgcggc ttagtgcatt gaatgcattg ttgcttacca ctatggccgg
     1501 tcggcggcct ttctgctcat gaccggattg cgggtcaaag tccaaataga taatatctcc
     1561 ttgctgcgga atataagcca ttacaacacc tcatttccgg taggagcccc ggtatcccat
     1621 tcagagaact cataaggtcc tttgaatccg accagacgtt cctccaatgt cctgtgtctc
     1681 atgccggctt tacggatgat gatggcatcc tgctgccgac ctatctcaac tctgtcactc
     1741 tcgcggaggt ctaaagcctc cagaagctgc tttggtaatc tgatcccttg gctattgccc
     1801 catttctgaa tagtggtgta catactgatc actcctttca tttatagtat atcctaagta
     1861 tatacaagaa gtcaattaga tatacgcgac cctgtccgta gtctataatt gtcctctaaa
     1921 gatgtgcaac aggctgtaaa gggtttggtt ctgtcttagc gggtaaacac atgccaaaat
     1981 aaaaatgaag ctctagaacg aaattagagc ttcatatcaa ttacgaattg atttcacctt
     2041 ttacttgttt tatgtggcct tattattatc taccatttcc cgcataacgc tgagaagctc
     2101 atcctggatc tctatggaga ggcagctgaa aagctgtaac ataaagatct cttttaatcg
     2161 ctgtgctgcc tcaaacctct gaataatcaa taccgacaat agtttctttc acctttacat
     2221 tttgcaacat gtttttctct tccttcaatt ttacagttat ttgttgttta tgaaaagttt
     2281 tgacacataa aaatctcttt tgcttccggt gatgatagct ggagaactga gcacattttg
     2341 cgtatttctc caatcaagaa ttctccactc ctgagcctac gactaaaaac atttatgcta
     2401 atactaatac caatttcagc agcaatagct tccttagttg tcgtatgctc agctattttc
     2461 ctgttcaatt tatcgacatc aacaaagtaa atatcttaac cccctctcac tattatttat
     2521 gttttacagc tgttgcaaca ccgattaacg gaacgatatt acacttcgaa ggtaatcaca
     2581 tgctaaagta ataccggttc tctaacgtca aataatagcc ttgctatgca ggtataaaca
     2641 aaagcctata tttgttgaaa ctgcctttta tcttataaac atattctgca agtgcttcgg
     2701 gtctgctggt atatgctcgc tgaccatcaa agcttactaa atgtggtctg cgttcctgaa
     2761 caaatgacag gatttccttg tgaccaataa cctcaaactt gccttgcaat ctactaactt
     2821 ctttttacaa ctccgaagtt ttcataagta gtagtctggt atatcatgaa ttatcactcc
     2881 tcatgcaaga tatatatgtt tataatccat ttctctcata ttttctctcc atctttccct
     2941 ttcttcttcc agcgctggat cgtcgagtca tctgctgggc gcttttcacc cctgccggag
     3001 cggacctgcc gtatcacttt ggccgaatag tgtttcccag atgcaatcac gttcgggatc
     3061 tccaggtgaa gagttccgca tgacagacac tttatccgac gcagcaggaa gtaataaggc
     3121 taaccgtcac ttcctattac gcgctagcgc cgggggtcat atccggagag cagctgccct
     3181 cagtccggac acatgggtgc ttgcttgttc tttactatgt agagtttcct ctcatcgtca
     3241 tagtcaagct ggtagtggtg tgtaataatc ataaaaaatt cctcctttcg atatgccttg
     3301 agcataaaac aaaaggagga acaattttca caggttattt ccgcgtaaag atacatccga
     3361 aaagtccgtt tacaaatata aaacgtacat gttcggataa gtctactttg gtgacccata
     3421 ggggattcga acccctgtat ccgccgtgag agggcggtgt cttaaccgct tgaccaatgg
     3481 gccatatatt taaaacttat atgagatttc ctaagctata atgaatagta caaaactcat
     3541 ataggaaact cataacataa aacattataa cactgcctta agataaattc aacataaatt
     3601 tttcacaaat ttttcgcaca atttagcgta tatatataac gaaaaataat tatacatgga
     3661 ataaattgaa tttttgcgta cctccattta ggtgcaagcg tgcaccttta ttcactaagg
     3721 taacataagc gtacaaactt ttgtaatata gtttcttttg tacgttatct atggtaattt
     3781 attccttaca attcattata aaagattatt gacatatgct cagaacggga tataataaaa
     3841 tagtaacgaa catatgtcga taacagtttg aatgcatacc tgtactcaaa atagtgcaac
     3901 ctgatatagt acggagtatt gtttttcatg ctgaaagtac atgactttag tcattaggtt
     3961 cactgaaggg gtgaaatgtt atggcgagac cgagaaaatg gcgaaaagta tgctgtttgc
     4021 caaagagcaa cttgtttggg ccgttaaacg cacaaattgt aagtgctgat actgtgttaa
     4081 tgacagtcga agaatatgaa actatacgtt taatagatct agaaggccta atgcaggaag
     4141 aatgtgctga aagaatgggt gttgcacgaa caaccgtgca aagaatttac tatgatgcgc
     4201 gaaaaaagct tgcacaatct ttggtaagcg ggagtgttct gaaaatagaa ggcggcgatt
     4261 ataagctttg cgacagaagt gaacaagcat atagttgtgg tcgatgccgc aggcataggc
     4321 acggtaacaa tgtagtgtat tcttaacagc acagcaaaat cttaaatatg taaaggagat
     4381 ataacggtga gcgaaaaatg taatcaaaat tgcagcgcat gcggtgaaga ctgcagtgaa
     4441 agaaaagaaa aaccggtgga tttttctgca aaaccacatg aattaagtaa tataaaaaaa
     4501 gttattggtg tcgtaagtgg aaaaggcggt gtagggaaat cccttgtgac ctcaatgctt
     4561 ggagtattaa tgaatcgaaa aggttataaa actgccattc ttgatgcaga tataacagga
     4621 ccatccatac caaaagcatt tggcgtcaag gaaaaagtta cgggaaatga attaggcatt
     4681 tacccggtaa aaagcaagac aggtattaat attatgtctg ttaatttact attggaaaat
     4741 gatactgacc ctgttgtctg gagaggcccc attattgcca atacagtaaa acagttctgg
     4801 acagatgtaa tttggggtga tgtagacttt atgtttattg atatgcctcc agggactggt
     4861 gatgttccgc ttactgtttt tcaatctatt gctgttgatg gaattatcat tgttgcatca
     4921 cctcaggaac tggtttccat gattgtatct aaagctgcta aaatggcaca gatgatgaat
     4981 ataccaataa tagcactggt cgaaaatatg gcgtacttaa attgccctga ttgtggcaag
     5041 gagattaaag tattcggaga tagccatatt gaagaaattg caaaaaaata taatatagat
     5101 aaactagcaa ggcttccaat tgaaccaaaa gttgcagcag cctgtgataa aggcttgatt
     5161 gaacttttta gcggtaattg gctggatgga attgtagaaa ttttagaaaa tatggaggag
     5221 aataaaaatg aagattgcag tcgcaagtga aaatgaaatg gttgcagagc atttcggaca
     5281 ttgctcgaat tttaatgttt ttgaagtaga aaacaattgt gttatcaaaa atcaatctat
     5341 acctaacccg ggacacaaac ctggattttt gcctaatttt ctcaatgaca ttggagtaaa
     5401 cgttattata tcaggcggaa tgggtggagg tgcaattgag atctttaatg aaaaaggcat
     5461 tgaggttata acgggtataa gaggaaaagc agaagcagca gtaaatagct atttacaagg
     5521 gacacttaaa tcttccggtt ctgtttgtca tgagcatcag catcatgatg aatgtggaaa
     5581 ttagtgttat tctgttacga gataagcttt agtatcacca gaaatagtgg gcataaacca
     5641 ataaattctt gataagggaa ataataaatt tatgaaggtc tatgttcgat taaatggaaa
     5701 ttcatcaggt aacatgatgg atcccagatt tggaagagct gcatttttta tttttgtaga
     5761 tattgactct ttgatgaaat cgcaggataa tgaacagaaa caaagtcttc gaatcttaaa
     5821 aagaaatggg cctattatgc ataaaaggtt tcttagtgaa tggatacctt tagaaaatcg
     5881 atattttaag gaaatgaaaa ttcaagagca gagtgattta gtcttttcag gctaaccctg
     5941 gaaggtgatc acgataaaaa ggcagcatat gctgcctttt tatcggaaaa aaacaacagt
     6001 aaaataactt gttgtttcgc tgcttcttgc acctgctatt tcatccgaat tgctgtggtc
     6061 aagcattttc atatgttctg ctcctgatgc attcatagct gagagcaaag cgataataga
     6121 aggcggagag tccatatagt cattattaaa atgttgaatt atatttatat ctctattttc
     6181 tattgcttta attgatattt catccatttg ccgggcttcc tcaaggggta ggtaatgaga
     6241 gaaatcaaca gaggcaatta tcgtactttt tttattttca ttttcaagtt tttcctgtag
     6301 gttttgacca agtttttggg cattctgtaa tccaaaactt ccatgcagca ttataggtac
     6361 aatttttgtg tcaggcatat aatacttaat ataggggacc agccctgcta tagagtgatc
     6421 ctcttcaagt aaatcaaaat tcatgtcagc cgttttagaa tctaccagtg agttcactat
     6481 atctacatcc gcttctaata taccaaaggg cgtctgccag ctccaacttc cggtgtgtac
     6541 tgcttttacc cctgtacctt tatgattagg tgctattatt ataactactt caggctgctc
     6601 tactgatatt gtcttaaaaa atgaggcaat catattactg gccagaagat ggtgagggac
     6661 tatgccgcct tttatgctgc tttctattat ttctgtttct gccagatgtc cataatcatc
     6721 taactgcacc ctatttacag attgcttgaa atccttttca ttatagtatc tgcactgaag
     6781 tctcttttca tttacctgta aattattaac aacctggcgc tgctggttaa atgaagcgcc
     6841 aggcattccc tccccgccgc tttgaatatt gctgctttga atgttgggtg acatagatgg
     6901 cagacgaagc aggagtgtat ctttccattg cccgaatatt ttgtagacag ttgcactgga
     6961 aattgcaata ctataatatg accccgaaag gagtaccata cataagccta ttatcataat
     7021 aataaatatt ttgtaatgct tttttaaatg tttcattttt taatctctac ctgtacctta
     7081 tcggcaaatc ccgaaatatc actcatacta tgcttaataa acgcatgatc tgctgtaaac
     7141 cggcccggtg ttactgcccg ggcataataa gtaatgcttc tctcgcgata cttgctgttt
     7201 ttattataat agaagccaaa tactatcttc tgaccatttc tttccacggg gtaccatttt
     7261 tggtcgaagt gcccagttgc tgatacatat cgcaatcctg cagggagaat atctgtaatt
     7321 tcataataac catcaggagc agtctctaaa aattccggct ccaaggttat ttttaccaat
     7381 tcagattggt taaagctcga ttgtactatg ccatccaagc tatagctcct ttttattttt
     7441 acctggctat ttgcatcttt tgcaaaatcc ttcactggtg ctgtataact tgaggttacg
     7501 gcaatatctc cgactacatt ggaaaatttt atattttgca aggcttcagg cgtaagaacc
     7561 agccggaaca ccctgttttt atccaaggtt atattttctt tccgcccgtt tacatctacc
     7621 gtaaagcttc cgttttgagc agtgtcaggg gtcatatgag caacgcatat caagttttct
     7681 atattagtca gaatatattt tgtagaatta tcctgtacat attttaacaa tccggctttt
     7741 tcaggtgcat tgattttgaa cccaagaaca gaacacagtg aggtaagctc aatagtatca
     7801 tccctacctg ctttagagtc aatatataca tatgggctgc ctgttacccc gtattgcttg
     7861 attatatcat cataaacgtt acgggcttct tttacattgc caaattcggc aagaccaatg
     7921 ctcaggtaca atttttcttt gattcctata tcatctgatt ttagcagatt ttcaatatcg
     7981 attaagacag gctcgttaag tgctgccaat ccccagagag aagctgcagc atcttcggga
     8041 gtgacatttc tattttccag ggttttgtaa aagtaccact tcattgatgt attatcaaat
     8101 aaactattcg ataaactatt taatgaacta atcgatgctg agttgccaga tgctgagttc
     8161 tttaataaca aatcctttga taaggagctg atttttgcag ttaattccgg gttactgctg
     8221 tcataggtaa gcagagctat accaccatca tactgctggt atgaattcaa ttgatagtct
     8281 tctgtgcctt cagcccacca tatatcatct ttatagtact tgctcattaa ttcccgggca
     8341 atttttctcg aaagcttctg atcaacccgc tccccccagc tatatagcaa tgaaagcagg
     8401 gtgttataat aggttgatgt tgcttcgtta ttgaataaga gggtcgtgag agactttccg
     8461 ccttccggct gcaaactgtc cgtaagcttg taatgctcta atttacttcc ttccagcaaa
     8521 ctttttacta ccttgaaatc tctttttatt gcatcactgt aattaccatg ctttgctttc
     8581 actgtaactg aataactgcc ttcttcaagc ttacccaaat ctatattggt caagtcatta
     8641 cctttacctt cagattgata tgttttcttt tcaagagtct tatttgcatc acttattgta
     8701 ccgctgctta cattattatt ttcaataccc tccttttcaa gcacaacagt atattctact
     8761 tttttatcac tttcaagctg tgtgccgaag cttctggcgg atatggcaac tgtatcccca
     8821 tccataaaaa cattattaaa tattacatca acaaaaaatg gcaattttac aggaatgtta
     8881 attttgccgc ttccggcttt taaatcatcg ctattcttct gataagcgat accctgatag
     8941 gtaattcgcc aagatgtcag gttatcagga agtttaaagc ttgctttacc tttaccgtta
     9001 ttatctgtag taatagtatc aaaaaaggcg ttatctttga atatgaacct ggtggattca
     9061 tctccgcctt ctcctcccat ttccggtgca ccaaaattga tattaatagg tttgtaagat
     9121 aagtattccg taagtattcc ggaattaaaa caatattgat atagagaagc cagggtattc
     9181 acttgttgac cataaacagc aaagaaagcc tcatctacaa cacttaagtt tacttcgcta
     9241 atgcatggag ttccttgtac atcttttaca tcaacttcca gctgaacagt gtcgccgggc
     9301 cggtactcat gcttatcagt ttttacatca attattaatt ctttctcttt gtaatcataa
     9361 ccaattaatg tattttccac atcaaatata ttcttaccat caaaatatac tgcctttaca
     9421 taaatatttg gtataaaatc ttttttaaaa ctaaaagaat aatcaggatc gttttcaact
     9481 atatgcgaga ctataccatc ttttaaaatc atataaagaa accggctgcc aggagttgat
     9541 gacagatatt cgttattctt ttttatattt aacgccacag tatcacctga cttgaacctt
     9601 ccaaactcca tatcattatg gtatcctgta ctaatagtat acctgtcaat atttccatcc
     9661 tgggggtgag catatctata taaatgtgct gtttctacta taggcttttg cctggaatct
     9721 gcaccttcta cttcaataac atagttttca ttttccttaa ttgggaactc atatgaatat
     9781 tcactattta gtgtgttggc cttgaattcc tgcaggagat ttttgacatt gaaataacgg
     9841 tatgttttac gaaccttttt gttaataaaa tcataatatt cgcctgtttc ttcacggccc
     9901 cagtgctgct cgtaaatctt aatatttaaa ggaatattta caaggtctcc ccggaattcg
     9961 tctgtattca tataccacga gtctgattgg cttctaagtt tttccaagtt tattcgattg
    10021 gtttttatct ctataagccc tgtttggcct cctgccattc cggacttgtc ttctgttttg
    10081 cctgttgttc ctgcttgatt tttggacgaa gcgtttacct ctatcattat atctcttggg
    10141 aaaactataa cattactata ggaatgaatt tcctcttctt ctgcttttgc attgtttaaa
    10201 ttcagactca aagcaatggg atgccacgag ttatttatcg atataggtaa tatctccagc
    10261 tctgctttac cattttcgtc gcaaataaga ttaccttctt tacgtgtatc ccatgtagtg
    10321 tagtgggaat attttaagtc aagacctgat acaggtgagc cttcaaaaaa actagcctgg
    10381 atatctacat ttagcttttc ccactcaaac attactttct tatccggagt aatatcaact
    10441 ttgtaggcag gctttgtata ttgtcttata gagaaatact tctgtataac tgactcgtca
    10501 ccaagcttga gctcaataaa atagctgcca ggattaaggt tggatatttc catactgctt
    10561 ttgaaagtac caagactaga tatatctatt tccttggata taatttctga caagcttgaa
    10621 ttttctccgt aagcataacc tgttccataa aaataatccg cacggtgcag acgaagtata
    10681 cctttagaag gtaaattgcc cttaattctt ggtctcacca tgccccagat ttgtatagcg
    10741 tcgctaggaa gatacaaatc tcggtcagtg tatagatatg cccaatattt ttgggagctt
    10801 ttgcggcccg gataatcgta atttgaatat tgccaggaat aatatgtaag aggggccagg
    10861 aaaaccgggc tatcgctgct attgtttttc gaaatactga agaacaacct ctgatctttt
    10921 tgccttttca gatctttaag cttattgcaa atctcaagta tatttcccgg tattattgca
    10981 acaccatctt tatcggtttt tactgtttga attacatcag atgacaacga attcgattgt
    11041 gcagaagatg atgataccga tggcgatgat gaagacggta aagatactgt ggataccgct
    11101 gcaccctcaa tcggttgacc agtgcttgaa ctatttatcc agacaagacc ttcctgtgta
    11161 cctgccatag catataccgc catatcgttt acctgaatgt gagtctgata tgtggtagtt
    11221 ttagatttta catttatcag gtagtgccct tccggtaaat ttgaaggaaa tattataaag
    11281 ctgccatacc agtagatatc gttattgttc ataaccttag tgttgaattc agttaccctc
    11341 tgaaggttag ttgtatcaaa tttgaggcta tcgcttctgc caaatgcctg ataggcaggc
    11401 gtattgtata gctttagatg ctcaagcaaa tcatctgtat tcgaatattt atatacctct
    11461 actgcaatct cctgatcttt aaagtcctgg cttgcatata ccgataatac aggctcagct
    11521 tgtggggtaa agttataaag tatgtctgtg aagcttaagt attctgtatt ttcctgttca
    11581 ggtgatttgc gtgtctcaaa tgcaaaaaca caatcttcgg ataggacttc atcacttccc
    11641 ttaataccta atcctttttt tacagttatt gtgtatatgg taccataatc caatttttcc
    11701 ggtatgaaga ccgctgtctt attatgatgt tcaaatcttc cattgacctt tggatttatt
    11761 tcaaaatact gttctatatt ttccacacct tcatggctaa agtttaattc tatacctgaa
    11821 tccacaggga cataggtgct tcggtcacca ggcagtgttc tgaccaatgc aaatgatttt
    11881 tttgtctgaa atgcccatga ataattataa tcgaatgtgt tggatgtatt gtcattgctc
    11941 ttgtctttat tattactcac tcctgttaag ctcatcttat aaatgctgtt gggctttaaa
    12001 gcatcgctaa aattaatctg aaattcctta tccgaaattt ctttaatctc ataattctgc
    12061 tgtggtgaga tggacagagt cttttgaaaa aatgacctgg attgcttatt ctcacataca
    12121 attttgaaac aggaatttat atctactcct gtgctgtctg ctgtcattgg gacaatatca
    12181 attgcaccgt cgattttcag gcttgaactg ctgccgctgg tataagaatc tccgggattg
    12241 aatgaagcag gagccctatc ggacagcaaa tttggaatta ccaataagga tattataagt
    12301 atagagatag cagtgatgac agaaacactg atgattaatt ttttgttttt aagcagttct
    12361 ttattattca aatttaaaac ccccgttttc gatattggtc ttatatatat tcacatattt
    12421 ttatcatact attatctaga ctattattca atgagaaatg ttccaatatt taggcgagct
    12481 tttatggagg acactttatg ggggagacac tttatggggg acactccttc atttttatct
    12541 ccaggacact tcttttttaa taaagaaaaa acttttaaat ttcttaaaag aaatggaaag
    12601 aaaagaagag aagagaagcg tccccggatt tccccggact tcaggctttt ttaatcgtac
    12661 ggtaaaactt ttaatattga caaaatacgt acgaaatatt ataataatag tacgataaag
    12721 agcaataata ctataccaag ccgtattata tttgtactga gaaattacta tccaacggag
    12781 ggatctttat gagtgaaaat attaattcat acaaagtaat aacagctacg gagttaaaaa
    12841 ataacctggg gaaatatttg gattatgtaa tggaagataa ggaagttgtt gtaacaaaga
    12901 atggcacaaa agcagtcagg cttacaccat atataaatga ttatgaccgt tatttcgtgc
    12961 tgaaggaaaa ggctcttgat tatttatacg gtggtttgaa agtttcatat gaagaattca
    13021 tggaaattta tgaaaaaagt gatttaagaa tggagtttat taacggtgag attgtgctgc
    13081 tatcttcacc cagcacaagg caccaggaaa tctcaggcga tttacatgta atatttaaag
    13141 aatatcttaa agacaagaaa tgcaaggtat tttatgctcc ttttgatgta cattttttca
    13201 aagtcggatt caaagaaccg gatgttatgc agccggatct gcttattgca tgcgatactg
    13261 atgaaaaggt taatgaaaaa ggacgttata tgggaacccc tgctttggtt gtagaaattc
    13321 tatcttctag tacccgctca aaagacatgg tagataaatt aaatacttat atgatttcag
    13381 gagtaaagga gttctgggtg gttgatcccc aaaatgaaag tattcttatt tatagtttta
    13441 aggattatga aatagatgat tttgttgcat acgcgaataa gaaagaagat gttgcacggt
    13501 cttttaattt tgaaggtttg gaagtcaagt gttctgagat ttttggctta taagagtaaa
    13561 ggtggtgtta gttttaaaga aattgataaa tttctggaaa actaatgcac atggggtgca
    13621 ttttcttcct atgatacaaa atatggcagc aaacgatttt ctgagtataa aatttgaaca
    13681 aagtttacgg attgttatgt ttaaggcaag ggaaaattcg taattcacga cacggatgtc
    13741 gtgagccgtc acctgaatca tggatgatga atgtgacggg tagaattttt ctgtagactt
    13801 aaacactaca atccgttgct gaagtgaata ttatacatgg aaatcgcttc ttgccatatt
    13861 ttccctcaaa tatagaaaat gtacccatgc acatatgcat attttttgca tatgattatg
    13921 ccccgaaaga aatcagttat gaggaattta ttgaaagata cgaaaagact gaggagaggc
    13981 ttgaatacat tgatagcaga gtttacgcca tgggttcacc gaatcatact catcaatcga
    14041 ttgtaggata tctgttcaat agtttttata ctttctttaa tggaaaggaa tgcaagccct
    14101 ttgtagcccc atatgatatt catttcgaaa attcccataa taaagcatgt gttcagccgg
    14161 atatatttgt tatgtgcgat aaagaaaatc ttagggatgg gaagtattat ggtgtacctg
    14221 ctctggttgt agaagtttta tccccggcat catgtaccaa agacactatg accaagttga
    14281 atttatattg gcgtgaaggg gtcagtgagt accttcttgt ggaaccgaga gaacaaatag
    14341 tttattattg gtatttcaga gaaaaggaat taataattca aaagacatta ggcgttgaac
    14401 atgtccttaa atcaaatatg ttcagcggtc tggaaataaa gatcagcgaa atattccaaa
    14461 gtatatattg aaaagtattc gaaaggatgt agcgaggaaa tggaacagaa aaatgaatca
    14521 ggacagaagg attttattat attggataag aaagacaatg ttgctgtagc acttaaagat
    14581 atggatattg gcgatataat ttcggtcgct ggtgatgcag ccatggatat tagtgacaca
    14641 attgaggata ttaatgatac gactgtatgt gacggaaaag cagaaagaat aaaagcaggg
    14701 cacaaagttg caattagaga tatcccaaaa ggagagaata taataaagta tggttatcct
    14761 attggacatg caacatgcca tataagtgcc ggtcaatggg ttcatactca taacgcaaga
    14821 acaaacctgc acgggatttt ggaatatact tataagtgca acacagattg caacatagat
    14881 tgcaaaacat tagtcaactc aggtgataca ggtgataaag catttagcag cccggacgac
    14941 aacaaggcaa acccattcac tttcaagggc tatttaagag aagatgggaa tgtgggtacc
    15001 agaaatgaaa tttggataat aaatactgtg ggctgtgtaa ataaaacagc cgaaatatta
    15061 gccagagaag caaatagaat cttctcaaat aagattgggc aaaattttga tggaattttt
    15121 gcatttcccc atccttacgg gtgttcacag ctaggggagg atcatttcac tactcaaaaa
    15181 cttttgtcag ctttggctaa acatcctaac gcagcaggca tattagtact gggacttgga
    15241 tgcgaaaata ataacataga tgaattcaaa aaaattcttg acgaaaaaaa taaagataga
    15301 atcaggttcc tgtcaaccca ggattgtgag gacgaagtca gctcaggaat caagcttatc
    15361 gcagaattaa tagattatac cgcccctttt cagaggcaga attgcccgat ttcgaaattg
    15421 actattggct tcaagtgcgg aggctctgac gctttttcag gaataactgc aaatccttta
    15481 tgtggagaat tttctgatat tattttagag caggggggta caacaatact tacggaagtg
    15541 cctgaaatgt tcggtgctga aaccatatta atggataggt gcgtaaataa ggacgttttt
    15601 aataaaactg tagaaatgat taatggattt aaagagtatt ttataagaca taaccagacg
    15661 atatatgaaa acccttctcc tggaaataaa gaaggtggaa tatcaactct tgaagaaaaa
    15721 tctctaggat gcattttaaa aggcgggtca ggtgctgttg cggatgtatt aaaatatgct
    15781 gacaaagtga aaaagacagg gcttaatctt cttgaaggcc ccgggaatga tgccgtatca
    15841 attacagctt taactgcagc aggtgctaat ctgattattt tcactaccgg cagggggaca
    15901 ccccttggag cacctgttcc tacgataaaa gtatctacaa caactgacct ctataataga
    15961 aaaggtgcat ggattgacta taacgcaggt caattactgg aatgcaagag cattaaatca
    16021 gtggcaaatg attttttctc atttattatt tctgttgctt caggggagat aaaaacaaaa
    16081 aatgagataa attgctatag agaaattgca atttttaaag atggaattac attatagact
    16141 aatatcggga caagcttcat ttgccttttg taccgaaatc tgagctaatg cagtagaatc
    16201 attttttatg ttttcaacct tgctaatgcc tgtcaaggta tattttttta aaatatacct
    16261 tgacaggcga agtatatatt attataatct atattagaaa gtgtggtgat aataatatgt
    16321 caattgcatc ggatatgata cgagggcata ctgaaacgat aattttaagg catttgctcc
    16381 caaatgatag ttacggctat gaaataaata aatgcgtaca agaaaaaaca gacaatcaat
    16441 atgaatgaaa agaagctact ctttattctg catttcgcag actggagcaa tcagggtata
    16501 tcatatccta ttggggcgat gaaacaacag gagcaaggag gagatattac tccattacgg
    16561 ctgaaggcag agtatactat gaaagatgtg ttgaagaatg gaaaaatgta aaaacattaa
    16621 ttgacaaact aatacaataa ttacaataag cataattaat gtaataagtg taataagaat
    16681 cctttgaaaa attatatgca taagaataca gcattaacca gcgtataagg acataaaaca
    16741 taatatgcaa gtgtttataa attaaaaaaa tgataaaatg ggggaattat aatgatcaat
    16801 aaaattcgtg catacattga ccatgcattt gaaggtatac ctcaaacaaa aaaagtactg
    16861 gaacttaaag aagagcttgt aggaaatctt atagaaaaat acaatgatta cctaaaaaat
    16921 ggaaaagctg aagatgaagc atataccgct gccatttccg gaataggcga tatctcggaa
    16981 ctagttgaaa gcatgaggga tgtagatcct tttgctcagc cttcaactaa ggaacgcagc
    17041 aggcatgcgc ttttcatatc aatagctgtt gctatttata ttatcagtgt atttatggta
    17101 cctatttata ccataaactt tggaaaacct gtaaccggaa tctttataat gttcctattt
    17161 attgccatag ccaccgggtt aattatttac agcaatatga catataagaa ttatattaag
    17221 atggacgata gtctggttga ggattttaaa gcttatcgtg caagcagcaa taagcaaaga
    17281 gcagcattta attcttttaa aagtgcattt tggagtatca ctgtagcagt atattttctt
    17341 ataagtttta tatatgggat atggggatat tcctgggtta tattcattat agcaggagca
    17401 atagaaaaaa tagtagcagg tattatacag ttaaggggtg aagaatgatg gataaaagat
    17461 taataaaagg caatattatt ctttggtctg tagttttggt tatagcaatc gccgtttttg
    17521 ttgcatttgc acggggaaac tactgcaatc catttggatt tccgttcaat atgaaaacgg
    17581 ccaatagatc acagtttgta gttgtaaagg aacaatccat taaaaatgat acacataata
    17641 taaatgatgt tacagtggat tggatttccg gtaatgtaga tatttttaaa agcgaaaata
    17701 atgaaataaa gattgttcaa ttagctggag aaaaattttc tgataatgat cttttctctt
    17761 ataaaataag gggaggaaaa ttatctgtta ttgatgaaag aaagggaaaa accagcattg
    17821 gttttaactt caataatttt aataatggtt ctgatttgga aatttatctt ccagaaaaag
    17881 agtttaattc tattagaatt aatactgtaa gcagtgatat aacttctgaa agtttaaata
    17941 ctgagatttt aacttttagt acaacatcag gggatgttat actttctggg aaattctcag
    18001 aggttgatgt taagacagtc agcggagata taagagctga aaatctggat gtacagagaa
    18061 ttgctctgga cacaacttcg ggtgacattt ctctgacggg cagcttaaca gaacttgata
    18121 tcaatacaat tagtggtgat gctaaggtat attcatcaga catgataaaa aatgttaagt
    18181 ctaatgcagt atcaggcaat gtaactatag ccattccgga aaatgatggt tttacaattg
    18241 acttcagcaa ggtttctgga aatttgaaaa gcgatttcgc ccttgtgact aacgggaaca
    18301 gacatagcta taaagatgga acagctaggt tcagtgctaa ttcggtaagt gggaatttta
    18361 caattttcaa aagataaaga ttgattttgg tgcaaaaaca attgtgtaag gaataaattt
    18421 agtgaaaaaa taaaattaaa aactatgata atatcttcac tttttaaaaa ttttgaccca
    18481 ggtcaaagtt tttttcttta tatacacgta taataagctc atagtaatta atactacatt
    18541 gctaatatgg tcaaaaacat attaagtctc gtaaaaaaga tattaaaaat ggaggtatat
    18601 tatcatgaca aacaaaacat atcaattaca aacattgacc tgccccagtt gcatggctaa
    18661 aattcagggt gctttaagaa aaactgcagg agtaaaagaa gcggaggtat tgtttaatac
    18721 cagtagggca aaagtaagct ttaacgaaga ccaggtatca tcagatacaa taaagaagac
    18781 aatagaaagc ctgggcttta agatacttgg cgaaaagtga ccggcagata tgcataaggc
    18841 acaaatacaa ccggatgcat ttaatttatg ctgtattagg gtaggtgtta gggtaggtgg
    18901 acactcctct ctcgagcaga gggtattcct tttacgttga ggagtgtccc catcttgcag
    18961 aatcattttt gggttataga cagcattaag caaaggaagg gagcggatag ctgtgattaa
    19021 gttaacaaag ggaaaaagag tcctaatttc aggcatcctt gttgcagtat cttttatttt
    19081 gaaaaacact attggacatc aactaattac cagcatagta atgatagctg ctgcaatagt
    19141 tgcaggaact cccataataa ggaatgcaat atcagcgtta agatacagga tacttggtat
    19201 agacgctctg gtatctattg cagttattgg agctatgttt ataggcgaat actgggaagc
    19261 tgccgctgta accttccttt ttatgtttgg tgaatatcta gagtccaaaa caattgaaaa
    19321 gaccagatct tccataaaat ctttgctgga tttggctcct gatactgcca gagtgatcag
    19381 agatggaaaa gaaattgaag tttctcctga aaaactggtt aaaggcgata tggcagttat
    19441 aaaaccggga gagaaaattc cggttgacgg catagtaacc gagggaacag cttatgttaa
    19501 tcaagcggct attacaggag aatctatacc tgttgctaaa aatatagaag atagcgtttt
    19561 ctcgggaacc ataattgaat caggatattt aatagtaaga gctgataagg taggtgggga
    19621 tactaccttt gcaagaatac ttcagatggt tgaggaagct caggacaaga aagccagaac
    19681 acaaaagttt cttgaaaaat tctcaaggta ctatactcct tctattatag ttttatctgc
    19741 attgctattt atatttacca gagatttggt attatctctc acgctacttg ttattgcctg
    19801 ccccggagct ctggttattt caactcctgt ttccattgtt gcaggtattg gcaacggagc
    19861 taaacatgga gttcttgtaa aaggcgggga gataatggag aaacttggaa gagttaaggt
    19921 gctggctttt gataaaactg gtactttaac aattggaaag ccagtggtaa accatataaa
    19981 agcttatgga ataaataaac atgagctgct taggatagct gctattggag aagcttattc
    20041 cgagcatccg ttggggaagg cgataattaa taaagctgaa aaggatttag gaactatatc
    20101 tcaagtgccg gaggaatcgg aaataattgc aggacagggg ttgaaggtat caatagataa
    20161 aagtataata ctaataggca atagaaaatt actgtcagaa aatggagtac aacttgacga
    20221 taatcatgaa aaatatataa gctctgaaga agaacatgga caaacagcag ttatagtggc
    20281 gaatttggaa agagtactcg aaataatatc aattgcagat aaagtgaggg aagatgcaaa
    20341 aaagttaata gctaatctta agcgtcttgg cgtaaaaaaa attgtgatgt taaccggaga
    20401 taataaaaga gcagcaaagg ccatttccaa tgaattgggt ataaatgatt actatgcgga
    20461 attgcttcct gatggaaagg taaagacctt aaaagaacta aaggaaaagt atggtctggc
    20521 ggcgatggtg ggtgatggcg tcaatgatgc gccggctctt gcttcagcgg atttaggcat
    20581 tgcaattggt ggcgcaggga aggatatagc catggaaacg gctgatatag ttatgatgtc
    20641 tgatgaaata aggaagctat cctttgcagt gggtttaagt cgtgccaccg taaggaatat
    20701 gaagcaaaat attatctttg ctttagttgt agccgggaca cttctgaccg gagtactaat
    20761 taaaacagta gatttagctt ttggaatgtt gattcacgag ctaagtgtgt tgttggttat
    20821 aattaatgca gtaagacttc ttggttatgg gaaaaaacaa aaatcagggt atgcttagtg
    20881 aggtaaatta ttatatgaac tgcaattgtc ataaaaggga agggagaaac tgcatagaga
    20941 tagtgcctat attcagcagc cttaatcatg aggaaatgat ggaggttgcc gggataactt
    21001 cagacaggac atatgaaaaa ggtgaaatga tttacattgc tggagataag ggggaaaagc
    21061 tttatgtcat tcataaagga aaagtcaaga tttccagagt gtctgagtct ggaaaagagc
    21121 aggttataag agttttgggt ccaggtgaat tcatgggaga gctttccctt tttagttcca
    21181 gccctctaac tgacaatgga gaagccttgg aaaagactac agtttgtatt atatcaaagg
    21241 ataaaattaa agaattgatg gcaaaatacc cggtaatttc cttcaaagtg atggaggaac
    21301 tcagcagaag gttggagaaa gtagaaaatc tggtagagaa tataagtctt catggtgtag
    21361 aaaaaagatt ggcagatact ctaatatcta tggttaatga taaaaatgaa gttattttga
    21421 agatgagtaa gaaggatttc gcctctcata ttggtatgag ccaggaaacc ttaagcagaa
    21481 agttgtcagc ttttcaggaa atgggattaa tcaagcttat cgggcatagg cggattatta
    21541 tattggataa agaagcttta agtgaaattg aataacctgt gtttctgttt aattttttga
    21601 agagcagaat tgtaagaaat tcttaagaaa aacctctaca tctttttaag aaatagcatt
    21661 tattatttaa acagggcgta ggggtttatt tatgctataa ttcaagctag cggattatgg
    21721 aagcagaaca aatggggggt tgatacatct gtgtgtaaga atgacgaaac cgaaagaccg
    21781 tcagtattga ttgtggatga tgataaggat attgtaaaag caatagccat aaaccttgaa
    21841 aatgacggct ataatgtctt taaagcatat gatggcctgc aggctctgga ttttatcaac
    21901 agtcagagta ttcagcttat tcttctcgat ataatgatgc ctaagcttga tggcctttcg
    21961 gctactatga aaatccgtca ggagaaaaat atccctataa taatactatc tgcgaagtcg
    22021 gaggatactg acaaaatact agggctttcc atgggtgctg atgattatgt aacaaaaccg
    22081 tttaatcctc tggaacttat ggcaagggtc aaatcccagc ttcgcagata catgacgctt
    22141 ggagacatag aagccaatgg gaaaactaat atactaaaat caggcggtct ttgttttgat
    22201 cctgacactc gaattgtaac agttgatgga gaaccagtga gacttacagc cacagaaatg
    22261 aaaattgttg agcttttact gaggagtccg ggccggatat tccccgctga agagatttat
    22321 gagcgggtct gggaagaacc ggcttacagc gttgaaaaca ctgttatggt acatattcgc
    22381 cgaattcgtg aaaagataga aataaacccc aaagatccca aatacttaaa ggtggtgtgg
    22441 ggtattggat ataaaatcga aaaaatctaa gggaatgata atttggttgt gtttttttat
    22501 aggggttaac attgttggct tcattgcatc aggtagtatt gttgaaatta taaatagcgg
    22561 ctatactgac actattgact ccataagaga tgtttataaa actgatataa aggatacaag
    22621 acagtttaag catacaataa ctctaatgtt taatatgctg gcaaaatctt tgaccgacga
    22681 caactattca tcaatgattg aagctatggg agaggattta aataaggaag aggaaaacct
    22741 tatattctat gctgaaaact ttaaaaccgg aaagagaata gccaattcaa aaaccgatct
    22801 tggtatttcc gttaaaggtt ttccttcact acttcagggg tatgattact atttttatta
    22861 taacggagaa gagttttttg cagggaattc tgataagcca cagaatattg ataaaaagga
    22921 tataggatat tcaaacttat atcattgggg ttgtttctcg gagaatataa atagtaagtt
    22981 tcccggaatt agagattgcc gcatattact tgtagtaaag aaagatataa caaaggttat
    23041 tagtgaaaca agccgtcttt acaacttgaa aaaaagattt caagttatga aatggaccat
    23101 tattggaatt atagttgttt tcctgtttgg agttgcgcta ttaataattt caatattaaa
    23161 tagaaaattg aaacatgagt ttgattttag gcttgcaaag ctttcaggga agttatggtt
    23221 tgagcccaaa gcattagtat cgttatttgt tattgcagtt attatggaag ctgaaagaaa
    23281 tacttccaag cttatgtata gcacccaact aagatttgct tttttcctga taatagccgg
    23341 ctggtggttt tatctaatgt ttattgattt agtgacaaac aaaagaaggt ttttctcaaa
    23401 caattcaata aatatgatta ttagaaaata tcgttccttt gaagggaaaa agccttttca
    23461 aaaaggaatg attttgcgcc tgtttatgtt tattggagca gaagctatac ttgttttcct
    23521 tgcaggagca ttttgcatta ttattattca aagaatggat tcctttttct tttattgtat
    23581 ttttatagtt gcagcaattg gtgtatatct tatttaccgt tatcagcgcc gatattccaa
    23641 tactataact gatataggca ggctggttga tcaaattgaa gcaattaaag ccggaggagt
    23701 aacagatgaa cttgttcttg accctgatac tgatatgtac aatgcagcta aaaacttaaa
    23761 tagtataaaa gagggcatga acaaagttat agaagaaaaa gtcaagagtg aacgtacaaa
    23821 agtggagctt ataacaaatg tttctcacga tctaaagaca cctcttacgt caattatcag
    23881 ttatgttgac ttattgtcaa aggaacaagg attacctgag catgtaaatg attatgttaa
    23941 aatattaata caaaaatcta atagattaaa aacactcatt gaggatttat ttgatttaac
    24001 cagagcttcc agtggtgaga taaaagttga ttatgaaagg cttgacctgg gcaagcttat
    24061 caaacagacc atggcagatt taaatgaaga gatatcccgt tcggaactgg cttttagggt
    24121 aaatatccct gagcagccgg tatttatact tagtgatggg aaaaagcttt accgggtatt
    24181 cctgaatctt atttcaaatg cgctcaaata ttctctccca ggctccagag tatatattga
    24241 cctgattgtt caaaatatga ataataagac taatggagag acaaaagaaa tagattatga
    24301 ggttgttgtc ataattaaaa acaccgcaaa ttatgaaatg agtttcagtc aggaagaaat
    24361 acttgaacgc tttgtcagag gagataaggc acgttctacc gaaggttccg gcctgggatt
    24421 agctatagca aagaatttca cgcaagtgtg cggcggaaaa tttgatatag tactggatgg
    24481 ggatttgttt aaggtgatat tatccttcgc tgcagcaaag tgaacactat ttcggagggg
    24541 tgcatttagt ttatctgaag aaaatattgg taaggggcat aagtgaaggt gctgtagagc
    24601 cttttaatct gagtgaagga taagcaaaat cagtgtttga acaaaataat tacaaccctt
    24661 ttgttcagat tattttgtga gtttgatttt gcccgtagct gaatttaaag gctctaagca
    24721 ctgtaacgat agcccctggt aaatatttct tcagatttga taaatgcacc tatttcggag
    24781 tataatattc attcataaac taataagtgg tataataaaa tcagtcatat taaaccatta
    24841 ttacagaaag gaattataag taaaatatat aatatttcac atatgaaact accagatgaa
    24901 tttttgacaa aaatgcagga aattttaaat aaagatgaat ataaagcctt tttaatgtct
    24961 tataatatgc cgaggtatta tgggttgcga gtgaatactt taaagataag tgtagagcgt
    25021 tttatggaga tatcgccctt caaacttgaa aggatacctt ggacaaaaga tggattctat
    25081 tataatgaag gtgaaaatcc agggaaacat cccttttatt atgcagggct ttattatatt
    25141 caggagccca gtgccatgct tcctgcaact gtgctggatg caaaacccgg agagtacatt
    25201 cttgacctat gcgccgcacc gggggggaag actgttcaga ttgctgcagg tatgaagggg
    25261 gaaggccttc ttatggccaa tgatacaagc gctgaaagat taaaagctct tgtaaaaaat
    25321 atagagcttt gcggtgtaag aaatgctatt gtgactaatg agcagcctca aaagcttgct
    25381 gccaagtttc cgggctattt tgataaaatt cttgtagatg caccctgctc tggagagggc
    25441 atgttcagaa aagacgaaaa tgctataaaa agctggggaa aatataagtg cgataattgt
    25501 gcagttattc aaaaagatat actggaatat gcagatgtaa tgcttaagcc cggtggcagg
    25561 cttgtatatt ccacatgtac tttttcacct gaggaagatg aaatgataat aggtaacttc
    25621 cttaagaaac ataatgacta tgatgttttg aaaatacctt taacagcggg aatagagaga
    25681 ggcaagccgg aatggggcgg gggagatgta cgactagcag gcactgtacg gttatggcct
    25741 cataaagtta agggagaagg gcattttact gctgttcttg agaagacagc tgcatttgat
    25801 ggccatatta atgacaaaca gactcaaaat caagaggaca attttagtga taattcattt
    25861 agcaaaaata tctatattaa agatatcaac tatagtagaa atatcaacca tagtaaagac
    25921 attaactccg gcatagatac tttgccaaaa gaatatgcag gatatttcaa agaatttatg
    25981 aaggacaatc ttaacattga aatagacggc aactttatgt taattggaaa taacctttat
    26041 tatttcccgg tacccttacc cgatctatca ggtataaagg ttgttaaatt cggatggtat
    26101 ctgggggagt ttataaatgt acaagccttg ggaagaccag tttctaatga gcaaacaact
    26161 attaagaagc aaagtccaca caagcaaaat gcaaataagc aaaaatcaaa ggagcaatat
    26221 ttaagggggc aatattcaaa agagcaaaat ttaatgaatc aaatttcaaa gaatttggtt
    26281 ctaaagagac gagaattgga aggacagttt gaaccatctc atcccttact gatatctcta
    26341 aaaatggatg atgttaaaaa cactagcaat tttacagctg attccagtga aatactaaaa
    26401 tatcttaaag gggagactct gattactgat ggtcaaaagg gcttaactgc agtatgtgtt
    26461 gatggtttta caatcgggtg ggcaaagcag acagggggta tgctaaaaaa tttatatcct
    26521 aaaggatgga gaaaacacag ataaaaacag acgcagatct ttatttattc acatcaataa
    26581 attacactca agctagaaac cgaaaactag aaaccgataa aaccagaaag ccagatgctt
    26641 ccaaaaccag aaaaccagaa gcttctattt gacaaatcga tagtaatgtc atataatttt
    26701 aaaaacagat tatattatgt aacccggaat ataaatatgg aattaatatc atataacagc
    26761 agttggctgc aattactgta attaaacaag tatgtgccgg caagtatatg ctgggatgaa
    26821 ttaaggaaat atattttctc aattaatgca atgtaatgct tatgctacat ggctttgcat
    26881 attgaatttg aaattttgca actatttgag aaaatcagta taggacgggt ttttgtttgt
    26941 tttatattta tctatgttat atttaactat tttaaggagt gacataaatg ggggtttact
    27001 taataaaaag aatatcatta gcagttgtta cactgttttt ggttgctacc ctgacctttt
    27061 tccttatgtt tcttgtaccg ggcggccctt ttcttgctga aaaagctcct acggctcaaa
    27121 cacttgctgc attggaaaag aagtacggtc ttgatcaacc tgtgagtgca cagtatatta
    27181 attatatgaa acgtcttatt cgcggtgact ttggtccgtc attgaagctt cgtgggcgtg
    27241 aagtaaatga agttataagt acaggattcc ctatatcagc gagtattggt ggtattgcag
    27301 taattctggc ggtgctagta ggtgttccat tgggcacttt ttctgctgtt aatcgaggaa
    27361 agtggctgga taacatagtt ttattcatat ctacacttgg tattgccata cccggttttg
    27421 tgttagctac aattcttatg tactactttg gtgtgacgct gcgtatcctt cctacttacg
    27481 ggcttagctc accgaagcat tttattttac cagtaatttc cctggccttt tatccaaccg
    27541 catatatttc caggttaatg cgttcaagta tgttggatgt gctaggtcag gattacatgc
    27601 gtacagccag ggccaagggt ctttcccagt ttaaatcctt attcaagcat gcattgcgta
    27661 atgctatact gcctgttgta acttatctgg ggcctttgct tgcttatata ttaaccggaa
    27721 gctttgtggt agagaaaata tttaccatac cggggctggg tggtcagttt atcagttcca
    27781 ttacaaaccg tgactatcct atgattatgg gtacaactat atttttggcg tttttaatca
    27841 ttttgatgaa tatggttgtg gatattgtat acaagattgt agatccgcga ataaagttaa
    27901 aataagaatt tgaccagtaa ttaaaccgga aattcatagg agaattgtat tgaaattcaa
    27961 ataagaatta aaacaaatat gaatacaatc atgtatgaac agatatgaaa taaattggaa
    28021 ttgaagtgag gagcgaaaaa cagaaaatga cacaaaaaat aggtgataaa atgaaccctt
    28081 taagtcttca tatagatgtt tctgattttt tacctgctga agagagtgaa aagcagagta
    28141 tggtagtaat gcgcgaaagt gtgaattttt ggaaggacgg atttcgcaga ctacgaaaaa
    28201 acaagcttgc tatgatttca ctgaccgtaa ttatagttat tatgatattt gcgtttattg
    28261 taccagtgtt ctatccttat tcttatgaac agcaaatcag aggcagcgag aatttaaatt
    28321 tgatggaata ctctaaagaa gagcttgcac gtaaagaaga aggcgagttt gttttccctc
    28381 atattttggg aacagacagt cttggtcgtg attacgctgt aaggataatg atgggcagca
    28441 gaatttcatt acttgtagga attatagcta gtgcaattat acttttaatt ggctcaatct
    28501 atggttcaat atcaggttac tttggcggga aagtcgattt aataatgatg cgtattgttg
    28561 atatcatata taccgttccg gatatcctga ttattatatt gctttctgtt acgctaaaat
    28621 atcctttaaa agatctagct gaaaatgtac cgggctttgg ctggataaac actgttggag
    28681 taggtcttat aagtatattt attgtatttt ctctattata ttgggttggt atggcacgta
    28741 tagtacgcgg tcagatactc acgctaaagg agcaggattt tattactgct gccaaggcct
    28801 tgggagcatc aaacagcagg attataagaa agcatctttt aactaactgt atcgggacat
    28861 tgattgtaac aactacatta cagataccat cttccatatt taccgaaagc tttttgagct
    28921 tccttggatt aggtgtaaat gctccgatgc caagtcttgg ctcattggcc tcgcaggctc
    28981 tcggaggcat taaatcatac ccgcatcggt tgcttttgcc ggctataacc attagcctga
    29041 taatattatc tttcaaccta ttgggtgatg gattacgaga tgcctttgat ccaaagctaa
    29101 agaattaagg agacgaaatg atgagtaata tattattaga tataaaaaac cttaaagtat
    29161 ctttttttac accggctggt gaggtgaaag ccattaatga tgtatcaatc caaatggaag
    29221 aaggcgatgt cctgggcatt gtaggtgaga gcggcagcgg caaatcggtc acatcctatt
    29281 caataatggg tttagtccct tttccaggtc gaataattga cggtactatt gatttcaatg
    29341 gtcatcgcat taatgatatg actgaagcag agttgcgttc tattcgtgga aacgaggtca
    29401 gcataatatt ccaggatccc atgacatctc ttaatcctgt atatactatt ggtaatcaga
    29461 taagagaagc aatacttctg catacaaatt tgaacaaaca acaggcatat gaaagatctg
    29521 ttgaactttt aaaacttgta ggaatcaatg agcctgaaaa acggcttaag cagtatccgc
    29581 atgagctttc cggcggtatg agacagcgtg ttatgattgc tattgcactg gcctgtgaac
    29641 ctaagctgct gattgcggat gaacctacta cagcattgga tgttactata caggcacaga
    29701 taatggagtt aatgacagaa cttaagaata agataaatat gtccattatt ctcataaccc
    29761 atgatcttgg tatagtatct agcgtatgcc ggaaaataat ggtaatgtat gcggggaaaa
    29821 tagtagaatc aggtacaatt gatgatattt tctacaatcc taagcatgag tacacgaaag
    29881 gtcttctgcg tagtattccc aagcttaata ccaaggaaca tgaaaaactg gttccaatcg
    29941 aaggaacacc tgtagatatg cttaatccgc ctgcaggttg cccgtttgct ccacgctgtg
    30001 agaagtgcat gaagatatgc ttgcgggaaa tgcctgaatt cacgaaaata agtgatattc
    30061 attattgtgc ttgctggtta ttacagaagg aagcatttga agcatcggca ggaggtaata
    30121 aatgatgggt aaatcaataa atcaaaagca taaatttgca acactcagag aaggagcatt
    30181 agttgaaata caaaacctta agcaatattt tcccatacag gacggtttat tcgggaaaaa
    30241 gtatgttaaa gctgtagatg atgtttcttt ctatattgat aaaggcgaaa ccttaggtct
    30301 ggttggcgaa tccggctgtg gcaaaactac tgcaggaaga actatactaa gactttatga
    30361 gcctaccggc ggcagaataa tttacaacgg cacagatata acttatgttg atatgatgcc
    30421 ctaccggagg aagatgcaaa ttgtttttca ggatccttat gcttcactaa atccacgcat
    30481 gaccgtggga gatattgttg gagagcctat tgatattcat aaattagccg cctcaaataa
    30541 ggagcgttca gaaaaaatca ttcaaatgct tgaccgggtt gggcttaaca gcgaacatgc
    30601 caatcgctat ccacatgaat tttcgggagg acagcgtcaa cgtataggaa ttgcccgtgc
    30661 tttggcagta gatcctgaat ttatagtttg tgatgaacct atatccgcac tggatgtttc
    30721 tatacaagca caaattgtaa atatgtttga aaagctgcag gaggaaatgg gactgacata
    30781 tttgtttatt gcccatgatc tgtcagttgt caaacatata agtaaccgta taggtgttat
    30841 gtatcttgga aagcttgtag agcttgcaga tagctatgag ctggtattcc atagtgttca
    30901 tccgtataca cgctctttaa tttccgcgat tcctgtaccg gacccaaaca caagccggac
    30961 acaaaagcgt atcatattgg agggagatgt accaagtcca ttaaatccgc ctagtggatg
    31021 ccgtttcaga acacgctgcc cttatgcgga taagatttgt gccgagaagg aacccgaatt
    31081 ccgggaaata gggaacaggc attttgctgc atgccatcat ctggataaag tgcattaaaa
    31141 gtaaagtaaa agtgcagttg atgaaaaaga tgaacccaaa atgatataat agtttacaat
    31201 aaatttcaaa tatgttaatc taattataat acatatttga aaataataaa atataaaact
    31261 aaaaaggaga ggaagaaaca tgaaaaaaat agctttactc ttggttatgg tcatggtatt
    31321 tggtacattc ctaagtgcct gtgggaacca aaagattgca tctacacctg aacttgtagt
    31381 ttgtgtaggt cctgaacctg atacaattga cccggctctg aactctgcag tcgatggtgc
    31441 aacactttta attcatggct ttgaggggct tatgaccctt gacaagaatg gaaaacccat
    31501 tgccggacag gcaaaatcct gcactcctag tgatgacaag ctaacctaca ctttcaagct
    31561 gcgggataat ttaaagtgga gtgatggtga accggttgta gcaaatgatt ttgtgtatgc
    31621 ctggaacagg gcaattaatc ctgaaactgc atcggactat gaatatatgt tcgagtctat
    31681 tgaaggctac gacaaaggtg ctcttaatgt tacagcgcct gatgacaaaa ctcttattgt
    31741 taagctggtt tcagcaacac cttatttcct cgaactatgt gcattcccaa catttttccc
    31801 tgtacgcaag gatattgtgg aagcaaatcc tgataaatgg gcacttgatc cagccacata
    31861 tgttggcaat ggtccataca tgatgactga gtgggtacat gattctcata tgatttacaa
    31921 gaaaaacccc aattactgga atgtatccaa acttggccct gatagcataa aattcatact
    31981 tatggatgat gataacgcta taattgctgc attcgaaaat gaggaaatcc ttttagcaga
    32041 ctccatgcca aatgatgaaa ttgatgcatg gagagataag aaagagttta atattgaagg
    32101 tcagcttggc acttactata ttagctttaa tgtaaagaaa cctcctcttg ataacgaaaa
    32161 ggttcgtaag gccttaatac ttgcagttga tcgtgaatac atcactaaaa acattggtaa
    32221 agccggtcaa gagcctgccg gtgcatatgt atcaataggt ctttcagatg ccgatcttaa
    32281 aaaagagttc cgtgaagtag gcggagacta ctatgatcct tcaggagcag cttatgaagc
    32341 aaaccttgct gaagctaaaa agcttcttgc agatgccggc tatccggatg gcaaaggttt
    32401 gccgaccatt gaatatctat acaacactgg tactggacat cagttgattg gtgaagctct
    32461 ccaggatatg tggaaaaaga ttggtgttaa tattgaactg gtttcccaag agtggaaaac
    32521 attccttaat acccgtaaaa acggtgaata ctttattgct cgtaatggct ggctgggaga
    32581 ttataatgac ccaatctcct tcctggatat gtggatcaca ggcggcggaa ataacgatgc
    32641 acaatggtcc aatgccgagt atgatgcatt aatttcaaag gttaagtctt catccgacag
    32701 agctgaacgt atgaaactta tgcatcaggc tgaagatata atattcaaag attctatgct
    32761 gtgcccaatc tactactatg ttgatattta cttgaagaac gagaaactgg atggtttcta
    32821 ttcttcacct cttgggtata agtactttat gtactccagc gttaaacaat aacttatgcg
    32881 atttggattg actaaaatat tgattgactt aaatatttat tgatttcaat attattagaa
    32941 taatacaaat ttcttgaaaa gctgatggtt tctacgaaat taaagtagag gccttcagct
    33001 tttttatatg ctttttttag ggaaggaaat aagttatact attatatata aatgaatggt
    33061 ataatgatat acaccaaggc tgatatattg ggaaatgcag cttgaattgc ttatgagatt
    33121 aaaaatatca ggtttacggt atgtatgtaa ttcaacataa ataacaaaat aataaaataa
    33181 taaaagaata aaagaacaaa agaataaaac cgggaaatag tgaaagaggt agagcatggc
    33241 aggtactcaa cgcttagata aaattttatc aaattttgga tatgggacac ggagggaagt
    33301 aaagcagctt gtaaagcagg gtatggtaaa agtagatgat attcctgcag ttgacagtgc
    33361 aatgcatgtt aaccctgaaa caagcgcaat agaagtaaat ggtaaaaaac ttaactacag
    33421 agagtttatt tatataatga tgaacaaacc ggcaggggtt atatccgcaa cttttgacaa
    33481 caagcttaaa accgttgttg atttgctgcc tgatgaatat aaagtgttca atccctttcc
    33541 tgtaggaagg cttgatatag atactgaagg acttttaata cttactaacg acggccctct
    33601 ggcgcatatg cttctttctc caaagaagag tgtgcctaag acatactatg ctcttgtaga
    33661 tggcagagta accgaggagg acattaaagc ttttaataat ggagttgtcc ttgatgatgg
    33721 atataaaaca cttcctgcac agcttaaaat tctaaagcag ggagagcagg gagcacattc
    33781 agaaatagag gttattattt atgagggcaa gtttcatcag gtaaaaagga tgtttgaaac
    33841 tgttggtaaa aaggtaaagt atctaagaag aatggccatg ggcagtcttg tgcttgatga
    33901 aaatcttgaa acaggtcaat tcagggagtt aacagaagaa gaaaataaga gtctatccat
    33961 ggtactacaa gccactactg ctatagtatc tggagctgcc atcgatatag tgcctgaagc
    34021 tgctgctgat agagaggatg attaaaaact gccacagaga attcatgata aaagcagaga
    34081 aagtctcacg gcttgataag aattctatga aacaataccg ggaattgaaa gatgaactaa
    34141 atatagaacc tgctcttgaa aggttaataa ggcttgattt atagtcaatg cctatccgaa
    34201 tgcactaata tagctatatt agttcagcct ttgtaaaacc tcatacgatt tatttcgtca
    34261 acaatggcct caatattttc aatggggaca tcaaatccta tttctatatt tatacctagt
    34321 ccgcctttag gcaggtacat tttctctata gtctcccgaa tatgatccct catatcttgt
    34381 ggtgtgccga aggggaaaaa ctgtctatca agatctagca taattggtat cttgccttta
    34441 caggttctta caagtctatc tattccattt gcactgtatt ggggatttat aatgttgacg
    34501 ccagcttcaa ctagatcagg cataatttct attatatcac cgtcagtgtg catataaaca
    34561 tattttttaa actggtggct tacatcatat atgcgcttaa aggcaggttt caagtagtta
    34621 cgccattttg ccgccccaat ggcaataccg ttctgcatac caaggtcatc gccaacatgg
    34681 atgatgggcg aatcgctttt ttcacagatt agcttcatct ggcgaacatt gtaatcacag
    34741 accttgtcaa taagtatttg aagttcatcg cattcctctg caaaatcaat catggcttct
    34801 tcaaaccctc ttaaatcaat aagtctcagg tacatgaagc catgtggcag accgatgtct
    34861 gattcaggaa cttttaaaat taaaatgcct tcacggtttg ggataggatg gcttgttaca
    34921 taggactcca ttccctcctg ttcattactc cacacacatc cccaggcatc agttagactt
    34981 ccataatgat agcttgcagg tagatttttt tcataatcat atttaagcta gtgatctcca
    35041 aagaaatctt catactttgc agttattttt tttatttcat tcccactttt tttcatggta
    35101 gcaggcaaaa aattaaaata aacaggaatt tcctcagggt attgataagt aatcgcttta
    35161 ataatgaggt cagtggtatt ttgcataata aacccctcct agcttttatc tcttttatat
    35221 ctatgataat atgatattta atattagtca tttgataaaa tactttgcac gattttaaag
    35281 catcagtgag gtgttgaaga tgttgttaag caaatacagg caagtattgt gtgaaggcac
    35341 actgaatttg ccgggtctta ccttatttgg acataatgtt acaacaaagg ccgttcttcc
    35401 tatgggaacc catattcatg acggctgcat tgaaatagta gtggttgtaa aaggcaacga
    35461 gtcgtattat gtagagaaca ggaagtttga actttcaggt ggagatgtct ttgtagccta
    35521 tacggaccag ccccatcgaa gcggaaatac ttcacaggga gtatgtgaaa taatatggtt
    35581 tcaaataaag cctgctgtac aagaaaattt tctcggacta tctgcgcctt ttggagatct
    35641 tattagatca aaactcatgg ctatagatac acatatatta aaaattgaca gagaagggtt
    35701 gttttatctc aggaagagtt ttgaaaactt tttaaggctc agggaggaga acaggatata
    35761 tgggcaaagt cttcttatta gttttctttc ccgcttgctt tttacacaga ctcaaaacag
    35821 aaaagatgat gagctaattg agaatataca taaatatatt gaagagaata tatatgatgt
    35881 gattaaaact gaagacattt gttcaaaatt ttgtatttct ctttcaggat ttaagcacaa
    35941 attcaaagag tatacagggg tgaccccccg ggactacatt aatgacagaa aaatacagaa
    36001 agcgaaagaa cttttacaaa gtagaaagag tgttacagaa acagctatgc agctttcttt
    36061 taattcaagt gattattttt ccgttgtgtt taagaaatat acggcatttt ccccaacgca
    36121 gtttagaaaa ttatagtcat tttagtgaag cggtatcctg ttacgagcca gataatttat
    36181 tctgaactcc tagtttaggt caggcagttt atccaaggcg aaatctatta tatcatcatc
    36241 catataataa atatcaggtt caataccttt accttgtatg tttatgcctt tcggagtgta
    36301 ccataacata gtggtggctt taacggcaaa cccttttttt agaggtatgg tgaattgacc
    36361 gattcctttt ccaaaggttt tatctcctat aagagtaaca ttgtcaaggt tgtcctttag
    36421 cgccgcaata aagttttctg aagcacttgc agtgttttta tcctgaagta taattattgt
    36481 ttcattttta atcacttttt ctttttttgc tttataggtt ttattaaaca acctcatttt
    36541 atcaactgaa attacactgc ccttgggaat aaagagatct gacattttgt tcattgccaa
    36601 tgtatctccg ccatagttgc ttcttaagtc tattataagc ctcggatact tattgatttc
    36661 agatacattg ttctgcaaaa atttttgcgt ataggtagag aaattggtaa tatgtatata
    36721 aactgtattc tcattcagaa cttttagctc agataaggat gcttcttctt tttcctcttc
    36781 tttgtacttt ttatactgct cgggtatata aagataagta tacctgtcct tattggtttt
    36841 ccggattttt tcagtaactg ctgatattac caaatcatcg aaatattgaa agtacctgcc
    36901 gcttacatcc cttttcaatt catttttata cagagtttcc agtgcatctg tgtatatata
    36961 gtgatgagaa ataaaatgct tgaaaaccaa ataatcgtaa ttcagataaa tataaaaccc
    37021 tgtacctgca ataaatgcga taaaaaagga tagaagaata gcaaatctct ttttaatttt
    37081 ttttagtttt tcttcaacag ttaaaatttt ttttgccatt tatttatctc ctgaatttaa
    37141 aatattcccg gcagtgacac tggcacataa agttaaatac aagtatggtc aagcatagac
    37201 aagtatggaa aagtatagaa aacgcagcaa attcaggtcg atgccaggta tataaaatta
    37261 gggtgagtta aggacccacc ctcctaagaa catagcataa tataattttc tgtagagtat
    37321 atgccattaa ggtttcgtaa tcagtttatc gatggcatcc tgattaaagc ctactattat
    37381 attgccatca atttctatta caggaacacc tctttgaccg gatttctgaa ccatttcaat
    37441 agctgcttgc ctgtcggcgg atacatcgta atcttcaaaa gctacgtttt ttgaagacag
    37501 gtacttttta gccaccgaac accagggaca tgcgggtgtt gaatatactt taacagtcat
    37561 gttaagctcc attcctgccg cgtgcgaaat gaattcgccc ttgcaggaac aaaacttttt
    37621 tattaaaaaa gtttttatgg gcttaaattc atttcagtcg cgcgtgcaat ttattttttt
    37681 atattaatgt gcaaaaaata tgcacatgcg cattagtttt tcagaaattt aacaatttct
    37741 ttcaaactat ttcctcctta aaaacctaat gtttagtatt atctataata ttaccattaa
    37801 ctcagaactt tattgctctt attacatata taccccatag gggtatatat aaacaagaaa
    37861 tcattcaact ttttccatat atctcttaat ttttttcgta gttgttttag caaattcatt
    37921 ttctcgtaaa tgaaaatcct ttacatactt atatgttacc agctttttat ttacctgttt
    37981 tacttccttt cggatcagct catgaatttc ttcctgtgaa agggtcttac cggcaaaatc
    38041 ctcctgtatt ttttccatat caggtacaac agctgctgat actactacat caccaaattc
    38101 agaatcgtcc ttgccataaa ccatggattc cataatataa ggacttttgt taaggagagt
    38161 ttcgatttcc tcaggataga tatttttgcc attcttagta acaataacat gtttctttct
    38221 gccagtaata tgaacaaatc catcgctgtc aataaacccc aaatcaccgg tataaaacca
    38281 accgcttttt aatacctttt ctgtagcttc agaatcacca tagtaaccaa gcataacatt
    38341 ttcgccttta actttgatct cacctacacc ttcctcattt ggcaagtcta tctttacttc
    38401 aaggcctgga agaggcaatc cagcagcatc atccttgcaa tatacgtcac gattaagagc
    38461 agctataggg ctgcattctg taagaccata gccttgaaca aaaatcaatc caaaatccct
    38521 gaatcctttt gctatatgtg gatctatacc tgctgccccg cttataaata accttatatt
    38581 tccgccaaaa ttattatata cgggagcaaa tagtttcctt gttatattta taccaaattt
    38641 attcaataaa ttgcttattt taatagctg
//
LOCUS       DAOSDD010000008        36580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_599_length_36580_cov_5.039179, whole genome shotgun sequence.
ACCESSION   DAOSDD010000008 DAOSDD010000000
VERSION     DAOSDD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 36580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36580
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_599_length_36580_cov_5.039179"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PK733_01405"
     CDS             <1..1186
                     /locus_tag="PK733_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ09234.1"
                     /translation="KYFKVSILTVIIFSFFTSLTGCYFFPQEEEVLAPPLKEPQKLEY
                     KTIKVKKGNIEKRINGTAYFVSDLQTDLCFKYRGGRIKSINVKDGDMVYEGDIIIELE
                     TENLENQIMQQEMALEKLKLSTGQTLSNLHRSIKLAELQLDDLKKKLEKIKKTMENLP
                     DGVSLQDIMPGANMEDIQDQIERQEFILNGEIEKYNNAETSAELDIKSIELQIQNLSI
                     ELEKTKLVSPVTGKVVWITSTKPGESIQAYTNLIRIADVNRLKLKYTGDNISDFKLGS
                     KVEVKIDDAEYEGEVVMTPSTAPFDADESIKRSIFINVKGLPKGVSLGYSARISYLLE
                     YKEDVIVIPRDMLHGFMGQKTVYILEDGLKKDRSVQTGIETPTEVEILKGLEEGEEII
                     EG"
     gene            1200..4061
                     /locus_tag="PK733_01410"
     CDS             1200..4061
                     /locus_tag="PK733_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09235.1"
                     /translation="MLIILIRKILSNKWMVLCLLIGSIITVAMVSSVPIYTNGVLQRM
                     LTRDLEGYQKNYGGFPGRYSIKVKLHYYTDNVDDRVKAYKWFAERIVDLPRQLNVPVI
                     IDNNSLIADNLTALPEIQREEKPKTRFFKFEGMDAFVEHAQIINGRMFSDKKEGDIIE
                     VVVTEKAFDSLDLRLNEVYHVSNFLNEDELLCKVKVVGTFTIKDAADLYWFDGIWNFD
                     ESFLMDNKLFSQLFIESQNPILTEVRWNYALDYHKIKIENISHILSAYDTQFRWFKKY
                     TAILDARLPIIPILEEYQKREKQLSLTLWVLQVPILLMLAFYMFMVAQLIVKSEENEI
                     AVVKSRGSSSLQIFTSYLLESILLGGIAFAAGPSLGLFICRILGLSNGFLEFVQRTSL
                     PVELSKTAYLYSLIALGLFVISMLLPAYLSSRTSIVLYKQSKVRKSKNPIWKRYFLDI
                     ILLAISGYGLYSYKMRTLTQVLTGIKGTDIAIDPLLFLISTMFILGVGLVLLRLFPYL
                     VSFIFWLGRKIWSPALYASFLHVGRSGGQEQFLMLFIILAISLGIFNANAARTLNRNI
                     EERIRYDIGADITIKAKWESNEVSFGGMMGAEPEMVGSVGFGSDNKPTEYIEPPFKQF
                     TELDGVELATKVFKKSNVEMRSIAGSTYNVDLLGIIPDEFGKVSWLRPELMPYHWYHY
                     LNLMAEAPTAFLVSSSTRDKYKLKEGDSIYLTWKGQTYLEGYIYAFIDYWPSYNPNFK
                     NQKGEPKDFIVANYSYIAARMSLEPYEVWIKREPGVTDTQVYNDIKEKKIAITDIKFT
                     KNEIIKKKNDPMLQGTNGALTMGFVITMLISSIGFLIYWVLSIRSRALQFGIFRAMGL
                     SMRNIITMLICEQILISGVAILLGIIIGGATCDLFIPLIQIAYSAEEMALPFKVFAYG
                     GDYIKLYTIVGTMLLSGIAILAILVSRININQAIKLGED"
     gene            4147..4878
                     /locus_tag="PK733_01415"
     CDS             4147..4878
                     /locus_tag="PK733_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09236.1"
                     /translation="MGEEMLAVKEKSIIRQAPIIVTDNLDRDFHSGREIVHALKKVNI
                     KIEPEKLTILRGRSGSGKTTLINLLGGLDTPTKGRIFFKDKEITSMTVSKRDELRKKN
                     FGFIFQSIAIISVMSAYENVEFGLRIAGFDSKHRRERAEECLTLVGLGKRMHHRAHEL
                     SGGEQQRVAIARAIAHRPGVIFADEPTAELDTYMGLQIVKLFKSLVEKEGLTVVMTTH
                     DPSMMEIADCVYTLEDGEIVDEQWA"
     gene            4862..5893
                     /locus_tag="PK733_01420"
     CDS             4862..5893
                     /locus_tag="PK733_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09237.1"
                     /translation="MNNGHDSINKQDKIQVDVHEGTQDDQSENIIICENLVKIYKTKE
                     TEVVALQGLDLTIEKGELMAIIGNSGSGKSTLLNMLGGLDRPSAGKLIIDGKDLLKFS
                     DRQLMKYRRETVGFVWQNNARNLIPYLTARENVELPLILTGKKKKAAKALELLDMVGL
                     TNRAYSKLSQLSGGEQQRVAIAIALANNPKILLADEPTGSVDTRTADQILDVFRVMSQ
                     TLGITVIIVTHDRRLARKVDRVVAIRDGRTSSEIIRKRSYAEELEGMKVGISSGDDDT
                     HVEYAVLDRAGRLQIPAEYIEAMSLKGRNKVKVSLEEGKIVLEPPEKSSDEKADEKAV
                     GKAVGKAVG"
     gene            6039..7340
                     /gene="eno"
                     /locus_tag="PK733_01425"
     CDS             6039..7340
                     /gene="eno"
                     /locus_tag="PK733_01425"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ09238.1"
                     /translation="MSFYLEIDSVYAREIIDSRGNPTIEVDVITEDGNLGRAAVPSGA
                     STGAFEAVELRDGDKERYLGKGVQKAVENVNTIIANEVEGMNVYDQVAIDNLMIALDG
                     THNKGRLGANAILGVSLAVAKAAAESLGLSLYQYIGGVNSKVLPVPMMNIINGGKHAD
                     NSVNIQEFMIMPVGACCFKTALRMCAEVFHNLKKVLSAKGYSTAVGDEGGFAPNLKTD
                     EEAIQVILEAVEKAGYRPGDDFRIAIDAAATEMYQDDGTYLFWKSNIRKTREEMVDFW
                     ADLASKYPIISLEDGVAEEDWEGWKLLTDKIGSKIQLVGDDLFVTNTERLKKGIDMGV
                     ANSILIKVNQIGTLTETLDAIQMANRAGYTAVVSHRSGETEDATIADIAVATNAGQIK
                     TGAPSRTDRVAKYNQLLRIEEELGASAQYLGLDAWFNLKNK"
     gene            7531..7773
                     /gene="secG"
                     /locus_tag="PK733_01430"
     CDS             7531..7773
                     /gene="secG"
                     /locus_tag="PK733_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ09239.1"
                     /translation="MKIGTIILNIIYIIISLSMVVIVLLQSAKSAGLSGSIAGGAETF
                     FGKNKGRTIDAMLSKYTTFASIAFIILSVVLYLLLK"
     gene            7964..10156
                     /gene="rnr"
                     /locus_tag="PK733_01435"
     CDS             7964..10156
                     /gene="rnr"
                     /locus_tag="PK733_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOJ09240.1"
                     /translation="MEEDRKKRILEYMESDTYKPFLFEELAGFLAVPDTELGIFRSVL
                     DELEAEGKVFKSKKNRYGLPKHFNLIAGRLQMNERGFGFVIPDNRNNGSNDSNGDIYI
                     SPEFMHGAMNNDRVYARIMYKGYYDKRAEGEIFRIIQRANEMVVGTFQSSRYFAFVIP
                     DDKRITGDVLIPKDEAGGAVDGQKVVAQIIRWPEESKHAEGCIVEILGDKNSPQADVL
                     SIIRTFNLPEKFPENVMQQVDKIGDTVPDSMLEGRRDLRQLRIVTIDGEDAKDLDDAV
                     SIEKTAEGYYRLGVHIADVSNYVVEGSPLDREALIRGTSVYLADRVIPMLPPKLSNGI
                     CSLNPGVDRLTFSVIMTINSSGNVIGHEICESVIKTTERMTYNNVYKMLVENDPQVCH
                     KYSHLIDDFKTMEELALVLRKKRIERGSMDFDFGEAKIILDEEGRPVDIIKFKSTIAE
                     KIIEEFMIVCNETVAEHFFWLEAPFVYRIHEEPDKEKLSNFSSFVRILGYRIKGKGEV
                     HPKALQDLLDEVRGKKEERLISTMLLRSLQKARYSEQNAGHFGLASKYYCHFTAPIRR
                     YPDLLIHKIMKQILKGEMSKEKTEYYDKHLQELARMCSERERAADDAEQAVDDLKKAE
                     YMEQFIGETFEGIITGITNFGMFIELDNTVEGVVRLSSMYDDYYYLDDMRYLLLGEKT
                     GKQYRIGDAIKVKVVKTDKIARQIEFELVNEPNKKHSSKKRSRKNESE"
     gene            complement(10299..10862)
                     /locus_tag="PK733_01440"
     CDS             complement(10299..10862)
                     /locus_tag="PK733_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ09241.1"
                     /translation="MIAIEPPPIGKNITILRKQKNMSLDELSKRSGVSKSMLSQIEQE
                     KANPTVVTVWKIARSLDVSISELMESNNNSPIEVIRRDDAPVIYSDDRSVKFRINSPV
                     HMTDNLEVYHTTFKALGKNKSNPHFPNAEEFFTVISGKFRVTSGDYSVILCEGDTARY
                     KGDREHTIENITESEAEAYLVVHFPKS"
     gene            11253..12296
                     /gene="tdh"
                     /locus_tag="PK733_01445"
     CDS             11253..12296
                     /gene="tdh"
                     /locus_tag="PK733_01445"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOJ09242.1"
                     /translation="MTMMKVVKKEEPGKGVSITTASVPEPGPEEVLIKVKLAGICGTD
                     LHIYKWDEWSKSRLKLPIIIGHEFVGEIVEVGSNVKHLKKGQRVSAEGHITCGYCRYC
                     QNGKGHICQDVKIIGVDTNGCFAEFITMPAGNVWPVHDAIPDRYAALFDPIGNAMHTV
                     MSQPVSMKTVLIMGAGSIGLFAIPIAKVNGAEKVIVVEPNPYKRDLAVKSGADLVIDS
                     NKTDIDSEIMSFTGNTGPDVFLEMSGNPGALRKGLDLLSKGGTASLLGIPEREVPINI
                     ADEIIFKGITIHGITGRRMFETWYQCQSFLLKDGKTIEPIITHTLSIDNIEDGFHLME
                     NNLAAKVLVDIEL"
     gene            12320..13498
                     /locus_tag="PK733_01450"
     CDS             12320..13498
                     /locus_tag="PK733_01450"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOJ09243.1"
                     /translation="MAKKFYNKLDEELDQLKNAGTYKSLKYLKSSLSGRAEIEGFGDV
                     IILCSNNYLGLADKPEIIHAGVETLKKYGAGAASVRFICGTYDIHRELEGKIAQFLGT
                     ESALTYTSCWAANTAVIPALLKSGDCVISDELNHASIIDGCRMVGREVKRFVYKHSDM
                     KSLEEKLKESEGTGTKLIVTDGVFSMEGDIANLPDIVEIAEKYGAIVMVDDSHATGVI
                     GKTGRGTEEYYNMIGKVDLITGTLGKALGGAGGGFVAAGKAITDICIQRSRPHLFSNS
                     LPPVMASIGIAAINYLDVNPSIVDSLHKKTAYLRNSLKQAGLKPLEGDSAIVPIILGD
                     TAKAIRVAENMLKKGVYAVGFGYPVVPEGTARIRIQVSDALTYEDIDIAVRVIREALL
                     "
     gene            13645..14736
                     /gene="gcvT"
                     /locus_tag="PK733_01455"
     CDS             13645..14736
                     /gene="gcvT"
                     /locus_tag="PK733_01455"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ09244.1"
                     /translation="MKKTPLYEQHVNLRGKIVEFAGWALPVQYSSILEEHEYVRNSAG
                     LFDVSHMGEIIIEGPDAEKLIQKLITNNIANMEQGKIIYSPMCYPDGGTVDDILVYKM
                     SNEEYFLVVNASNTDKDYQWMLDNAEGDAEITNVSSNYAQLALQGPKAMEILGRLANF
                     NLEKIRPFRFEAKVDIAGFEALVSRTGYTGEDGFEIYIAPENAEKLWNKILDEGKEDR
                     LAPAGLGARDTLRFEAGLPLYGQEISKEITPVEAGLSRFVKFDKGDFIGRSALLAQKE
                     NEPGRKLVGFEMIERGIPRSHYEVQSEGKSIGFVTSGNFSPTLKKNLGLALISQEYSD
                     LGEVIEIVIRNKTLKATIIETPFYSRKRG"
     gene            14741..15124
                     /gene="gcvH"
                     /locus_tag="PK733_01460"
     CDS             14741..15124
                     /gene="gcvH"
                     /locus_tag="PK733_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOJ09245.1"
                     /translation="MKLESGLKYSKEHEWVRVAGNKAYIGITDFAQNSLGDIVFVEMP
                     EVGAELASGDTLGVVESVKAASDIYTPVSGTVTKINENLNNSPESINEDPYESWIIAL
                     ELSDPAELDDLMDEKEYEQFCSKEG"
     gene            15176..16534
                     /gene="gcvPA"
                     /locus_tag="PK733_01465"
     CDS             15176..16534
                     /gene="gcvPA"
                     /locus_tag="PK733_01465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOJ09246.1"
                     /translation="MRNYTSRYIPNTEEQQKSMLETMGMKSLDEIFKDIPEELKLNRA
                     LNIPDDMAEYELIKHMQNMASKNANMDDYVCFLGAGAYDHYIPSIIRHMLMRGEFYTA
                     YTPYQPEISQGTLQAIFEYQTMICELTGMDVANASMYDGASAATEAIILACNGKRKNE
                     VVISAALNPEYKKVINTYARFNGFKVIEAAYCNGITDISDLEKKITGDTAAVFIQSPN
                     FFGAIEDLTNAGQIAHKNDIYFIVSVDPISLGILKPPAEYGAHIVVGEGQALGNPMGF
                     GGPYLGFFAVKKELMRKMPGRIVGQTTDVNGKRGFVLTMQTREQHIRREKATSNICTN
                     QALNALTATIYLTVLGKKGLKEVGELCVQKSHYAYDRLLETGKFEAAFTSPFFKEFAL
                     KAKNGISIEELNMKLLNDKIIGGYDLGKDFEEMKDGWLVAVTEKRSKEDIDRLVEKVN
                     CL"
     gene            16681..18138
                     /gene="gcvPB"
                     /locus_tag="PK733_01470"
     CDS             16681..18138
                     /gene="gcvPB"
                     /locus_tag="PK733_01470"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOJ09247.1"
                     /translation="MNDRALIFEISKPGRMAYSFADCDVPEKNIEQLIPREYMRENAP
                     ELPEVSEVEAVRHFVGLSRLNHAVDTGFYPLGSCTMKYNPKVNEDVAALNGFSSVHPL
                     QPENTVQGCLELVYRTGEMLAEITGMEKVSLQPAAGAHGELTGLMIMKAWHIHNGQEQ
                     RRKIIIPDSAHGTNPASAAFAGFEVIQVKSNERGGVDLDSLKSIMGADIAGLMLTNPN
                     TLGLFDENIKQIAHIVHEAGGLLYYDGANANAILGITRPGDMGFDIVHLNLHKTFSTP
                     HGGGGPGSGPVGVTARLVPFLPSPIVEFRDGKYRFDYNCPLSIGKVKSFYGNFAVVVK
                     TYAYILSMGPEGLKMASETAVLNANYVMNKLKEYYELPYERSCMHEFVISAQKQKNLG
                     VSALDVAKRLMDYGIHPPTIYFPLIVKEALMVEPTETESRETLDEFAEVMIKIAEEAE
                     HDPETVKNAPYSTPVGRLDEVRAAREPVLRWDKEK"
     gene            complement(18270..18497)
                     /locus_tag="PK733_01475"
     CDS             complement(18270..18497)
                     /locus_tag="PK733_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ09248.1"
                     /translation="MKSFNIMLKSINDVKDFVNIVNKYDFDVDLSSGRYVVDAKSIMG
                     IFSLDLSKPIKVEAHVDDCQGFCDEIKKFAV"
     gene            18738..20039
                     /gene="mtaB"
                     /locus_tag="PK733_01480"
     CDS             18738..20039
                     /gene="mtaB"
                     /locus_tag="PK733_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOJ09249.1"
                     /translation="MKKVAFCTLGCKVNQYESEAVSNLFENAGYTIVDFDEIADIYVI
                     NTCTVTGISARKSRQMVRRAKSLNREAIIVVMGCYSQTSPEEVSNIPGVDLIIGTSDR
                     NKIIEYVEEITQCGKQLNTVEGIMKKRDFEELHVDKYKERTRAYLKIQEGCTQFCSYC
                     IIPYARGPVRSRNPEEVLKEVKRLSDNGFKEVILTGIHVASYGRDLRNTSLLDIICRI
                     HEVDGIERIRLSSIEPTTITNDFVQIVKNLNKLCPHYHISLQSGCDETLKRMNRKYDT
                     SEYRSVVERLRDNINDVAITTDVMVGFPGESDNEFEQTYEFLQEICFSKMHVFKFSPR
                     KGTPAYSFTNQVPGDVKEERSRKLIELSDECALKFQRQFEGRTMPVLFEQEAGENSIE
                     GLTPNYIRVFCRGSNSLKGEIRDVVLKNTYNDFMDGEIINP"
     gene            20206..20466
                     /locus_tag="PK733_01485"
     CDS             20206..20466
                     /locus_tag="PK733_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOJ09250.1"
                     /translation="MVNNETMMFNVDKDNADETHEILMTIYQALKERGYNPINQIVGY
                     ILSGDPTYITNHKNARSLVRKLERDELLEEVLKFYLESNKNR"
     gene            20591..21538
                     /locus_tag="PK733_01490"
     CDS             20591..21538
                     /locus_tag="PK733_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ09251.1"
                     /translation="MEYIKLGNTGIEVSRMCFGALIIGPLQVNLPVDRGAEIIKRGLE
                     LGINFIDTAELYGTYPHIREAVKRSGKRPVIATKCYAYTKEGAAESLEKARRELDMDV
                     IDMFLLHEQESRLTLKGHKEALDYFISAKEKGIIRAVGVSTHNIEVVEACSEMPEIDV
                     IHPLVNIKGIGIGDGTIDEMLKAVKKAYGAGKGIYSMKPLGGGNLLGSFEDCIKFVLN
                     IPYIHSIAIGMQSEEEVEINVGIFESHEIPEELKARIRKINKKLHIDYWCEGCGKCVE
                     RCKQGALEIVDSKAKVNYEKCVLCGYCGSVCPQFAIKIC"
     gene            21602..22057
                     /gene="ruvX"
                     /locus_tag="PK733_01495"
     CDS             21602..22057
                     /gene="ruvX"
                     /locus_tag="PK733_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOJ09252.1"
                     /translation="MRIMGIDYGDARIGIAISDPFGWTAQGLDTIDWKNDINYPIGKI
                     INIIKEYGIEKVIVGYPVNMNGTLGERALKTEEFIKQLVIRTGNIDIIKWDERLTTVA
                     AHRILSEAGVKASKRKKCVDRIAAIYILQGYLDSLRNNYSKNTGDTEMQ"
     gene            22149..22502
                     /locus_tag="PK733_01500"
     CDS             22149..22502
                     /locus_tag="PK733_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOJ09253.1"
                     /translation="MPEERDDIVVLVDENGEEEEFEFVDTVEMDGNEYVILSPVSEDE
                     NDNDDFDEEVVILKVDKKEDGEESFVTVENEDELDAVFEEFKMRIEDDYYFDFDEEDE
                     DDEDEEEDDDDDDEL"
     gene            22749..23987
                     /locus_tag="PK733_01505"
     CDS             22749..23987
                     /locus_tag="PK733_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOJ09254.1"
                     /translation="MKEFFTRTTSILQKRKIAVASIAVVILLIFSLIVVIVSHNNRQS
                     DENTAKQPEESESNNFQDNEQDMAENNNSKDESFAFPISGVRPYAVMIDNEGTKCLPQ
                     GGLHKAQVIYEIIVEGGETRFMPVFWASLPSEDSKESKVTQQETQGTKETNGTQEAKD
                     IQNIQNARFPELELIGPVRSSRHYFLDYVLELDAIYVHFGWSPRAMSDIPKLKINNIN
                     GVANGGEIFWDLTKDRNNWQDSYTSMQKINEYVKKVKYRTETDKKSVFSYNKEDVEIP
                     GGVDAKSVLIKYNQANTSQYLYNAETREYSRLRKGKPHMERATEEQLKTKNIIIQFTK
                     NYTIKGDTEDRQEVETTGNGEGWYITCGKAVKVKWTKKSRNEATEYKYESGEPVLLNP
                     GQTWVQIVPIYGNVEIKGEL"
     gene            23991..25178
                     /locus_tag="PK733_01510"
     CDS             23991..25178
                     /locus_tag="PK733_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOJ09255.1"
                     /translation="MAIHDEVKKIADDIYAKLIDIRRGIHKFPELGLKTYRTAELITG
                     HLTGLGVEVTKAADGSGVVGLIRGKAGSKTVALRADIDALPVNEENSFEYRSCISGAM
                     HACGHDFHTACLLGASEILYRLKDKINGNIKLLFQPGEEGLDGALAMIEEDVLENPHV
                     NACIAAHVWPYVPVGKIAVIYGPSFSAINNFIITITGKGGHGAMPHDTVDPIIAGCNI
                     INALQTIKSRRIDSFEPATVSICSFNAGTSVNIIPDTAVIGGTLRTFNLDLRKKIETM
                     ICEISKGIAEPMGAKVSVQFMNEAPPVMNDREITELFINSASKIAGKENIITDIRPAM
                     VSEDFAFFSEKVPSTYFWLGCRNEEKGMIHPLHSSNFQGDEECIKIGAAAFAQFAIDY
                     LAI"
     gene            25288..27228
                     /locus_tag="PK733_01515"
     CDS             25288..27228
                     /locus_tag="PK733_01515"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ09256.1"
                     /translation="MKEKRKHGLKICWKQEEQTLEFAARELGRYAEMMMENNLDVTYS
                     KSCSCCEPEKEAISLGLFENFGITSKEVENTAFDDEIHIDIENGKGIIAGINPRSVLL
                     GVYRFLTEAGCMWVRPGKDGEFIPAKNIFNISVKLHERPSYRHRGICIEGANSYENIE
                     QIIDWAPKAGFNAYFFQFREAYTFFERWYSHTNNPLKKPEEFTVEMAREFLKRAEKEI
                     EKRGIIYHAVGHGWTCEPFGIPGLSWDKADYIVKPEVEKYLAEVNGKRELWGGIPLNT
                     NLCYSNTEVRNTITGAIADYLEENTTIDILHFWLADGSNNQCECENCRKKLPSDYYVM
                     MLNELDEILAARGIDTKIVFLIYVDLLWAPETEKIKNPDRFILMFAPITRTYSMPFET
                     DEELPEPPAYKRNQLKFASSVKENLAFLKSWEKAFKGDSFDFDYHFMWDHYADPGYYK
                     IAEILYKDINNLKGIGLNGYVSCQTQRAFFPTGLGMYIMGRTLWDSSLDFNVIRDSYF
                     QRAFGIDWEKCRDYMVDLSKLFDPPYLRKEKEIVSSEAAERFSGICTFVDEFRKVIDS
                     NVSSENPCHAASWNYLKHHSDLCCILALVLEARALGNNAKASAIWKLAEKYVQEHEDN
                     FQTVLDVYLFVSTIKGRLGFSK"
     gene            27326..28603
                     /locus_tag="PK733_01520"
     CDS             27326..28603
                     /locus_tag="PK733_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ09257.1"
                     /translation="MKDLRNMSVDELNNMKEKLQKRYDDFKKQNMKLDMSRGKPGADQ
                     LELSMGLFEYADHEHYRASDGTDCRNYGGLDGLPEMKKIFADVLDVTPAEVIVGGNAS
                     LNLMYDLISQAMTHGVAGSEVPWGKLPAVKFLCPSPGYDRHFAICEYFNIEMIIVPMK
                     SDGPDMDMVEKLATEDEYIKGIWCVPKYSNPDGITYSDEVVERFARMKTKSSDFRIFW
                     DNAYVIHHLYDQHDELKNILKACKEAGNPDRVFMFTSTSKITFPGAGISAIASSENNI
                     AEIKKRMTRQTIGPDKLNQLRHVAVLKDLNNINEHMKKHATILRPKFDVVLDSLEKEL
                     GEKNIAQWNKPRGGYFVSLNVMNGCAKKVVAKAAEAGVKLTSAGATFPYGKDPNDSNI
                     RIAPSFPSLSELEKAIELLCICVQLVSIEKILE"
     gene            complement(28691..29338)
                     /locus_tag="PK733_01525"
     CDS             complement(28691..29338)
                     /locus_tag="PK733_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOJ09258.1"
                     /translation="MKSYLVLGLGRFGESLAKTLSKHGHDILAVDKNEDIVQQFSSEV
                     THAIVAESSNEEFLKSVGIKNFDAVIVAIGDIQSSILTTVLLKELGAKYVLAKASNEL
                     HAKVLYKVGVDKVVFPEKDMGIRAANSILYRNIIDVMELFPEYSIMEITVPSTWIGKT
                     IRDLSVRSKFGVNIIALRKIQNLNVLPNADAKFDEGDILVVMGKNSDLKALQNLK"
     gene            complement(29555..30940)
                     /locus_tag="PK733_01530"
     CDS             complement(29555..30940)
                     /locus_tag="PK733_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOJ09259.1"
                     /translation="MNIFKFNTVKDGEEKLTHFSPTKALVFGFAFLILAGAILLSLPV
                     STKPGYSVGFLDAVFTATSAVCVTGLVVVDTNTTWSAFGQTVIIILIQIGGLGIMSLA
                     TMFSLVLGRRIGLKERLAIQESLNEFTLTGIVRVFKKILYATFAIEFIGAAVLSTRLI
                     PLYGLKSGIAKSVFHSISAFCNAGFDIFGSVGNEFISLTQLNREPVIIITIGLLIIIG
                     GLGFIVWKDVVQNRKFSSFMLHTKIVLIVTTILIVSGSVLFFVFESNNPETLKGLPWA
                     DKILNAWFHAVTPRTAGYNSLPIAQMTEPSKLMTIFLMFVGGASGSTAGGIKVTTFSV
                     IIFATLSFIKGEHDVNLLSRKIPDTVLRKSLAIVTLSSALVFATTIILLINKEGTLME
                     ALFEAVSGFGTVGLTTGITPGLKVISKLQLILTMFLGRVGPLSAAMIISFGQGSKILP
                     YKYPEGKISVG"
     gene            31369..32454
                     /gene="asd"
                     /locus_tag="PK733_01535"
     CDS             31369..32454
                     /gene="asd"
                     /locus_tag="PK733_01535"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ09260.1"
                     /translation="MDKKFRVGVIGGTGFVGQRFVTLLQNHPWFEVACIAASERSAGK
                     TYAEAVEGRWKLNIPMPAELGNITVKNINQVEEVTGEVDFVFCAVDMKKEEIKAFEEL
                     YAKTETPVVSNNSANRWTFDVPMVIPEINADHLSIIESQKKRLGTKRGFIAVKPNCSI
                     QSYVPAIHPLMDYGPEKIIVSTYQAISGAGKVFKDWPEIMDNVIPYIGGEEDKSENEP
                     LKIWGSISGNEIVKAQKPVISAQCYRVPVSDGHMASVSVSFSKKPSESDILEAWKSYK
                     GKPQILQLPSAPKQFITYFEGNDRPQTKLDRDCENGMGITVGRLRNDNILDYKFACLS
                     HNTIRGAAGGAVLIAELLVAEGYIARK"
     gene            32807..33427
                     /locus_tag="PK733_01540"
     CDS             32807..33427
                     /locus_tag="PK733_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOJ09261.1"
                     /translation="MQEIATINIESRENTGGGSSRRLRNNGYIPAVLYGKGMQPLPVK
                     VKKSELRGTLLKYGKNAVFSIKLPGKDDTSAIIKEIQNDFATGEIMHADFHQISLTEE
                     IKVEVPVKILGREIVEAARMVVLQQMDEISVKCLPQNAPQHIEIDVSKMSPGESIAAG
                     DVKLLEGVALESQPDQIVVTLNEVKENKAEEENTEAEETETAAANE"
     gene            33686..35368
                     /locus_tag="PK733_01545"
     CDS             33686..35368
                     /locus_tag="PK733_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOJ09262.1"
                     /translation="MPAELDLKEIIFGILGGLALFIYGMNLMSDSLQKAAGERMRRIL
                     EVLTGNPFIGVLVGALVTAVVQSSGATTVMVIGFVSARLMTLTQAIGVILGANIGTTI
                     TAQIVAFKLGSYAYVIAALGFVIYFLFKKRTIKYIGQSIFAFGLLFIGLNLMGDVMKP
                     LAQSSEFASIMLRLNKNRFLGVLVGLVSTVLVQSSSAVIAVVQNIAAHPAVDGVHAMI
                     NLETAIPILFGSNIGSTVTALLASIGGKINAKRAAVAHFIFNIFGTLVFIWFIPAFAK
                     LVKVISPKGDEVLIVSRQIANAHTSFNIVNTIIWLPFIWLMTKIVTFIVRGEDDEIEK
                     RVLYLDNRMLNNPAIAMNLVTRELARMAKMAQQMMTSARQAFVMSNMEESKKVFEIEE
                     TIDTLQTEIVKYLSTMLSRASLTERQSIRLAGLMHITNDIERIGDHCENVAEFAEWKV
                     NENIPFSQEALSEVADAFTMVNNMVDDSIYALHDGNIELAKKVLSEENEVDNLEERLR
                     DRHLKRLNSGLCDPQAAVIFIELIHNLERIADHCNNVAEAVLSEYNIKPETI"
     gene            35570..36445
                     /locus_tag="PK733_01550"
     CDS             35570..36445
                     /locus_tag="PK733_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ09263.1"
                     /translation="MGDNRYKKINQVLWIVLLLNIFVALIKIITGYIIKSGSLTADGF
                     HSMTDSSSNVIGLFGIKLASKPVDEDHPYGHKKFETMAGLFIAVFLLILAGKVIPDAA
                     RNLTHPAAPQVTVESIIALVITLAINILVSKYEFKQGLILESDILISDSKHTKSDIFI
                     SIGVIMTLIGIKLGLPPIIDPIISLVVAGFILYASYEIINSTGKILLDKVAIDEEKIR
                     DIVMEFKEVKGCHKIRSRGRNDDIYIDLHILIDPGMSVEESHTLTHGIEMKMREEFNK
                     NVQVIVHIEPYYEFH"
BASE COUNT        12653 a         5544 c         7730 g        10653 t
ORIGIN      
        1 aaagtatttc aaagtatcca tattaaccgt aataatcttt tcatttttta cttcacttac
       61 aggttgttac tttttcccac aggaagagga agtgctggct ccaccattaa aggaacctca
      121 aaaattagag tataaaacaa taaaagtaaa aaaaggtaat atagaaaagc gtataaatgg
      181 aacagcatat tttgtatctg atctgcaaac tgatctttgt tttaagtaca gaggtggaag
      241 aataaagtca ataaatgtaa aagatggtga tatggtttat gaaggcgaca ttataattga
      301 gcttgaaaca gaaaaccttg aaaatcaaat catgcagcag gaaatggctc tcgaaaaact
      361 taaactaagc acaggtcaga ccttgtcaaa tttacataga tcaattaaac tggctgaact
      421 tcaacttgat gacttgaaga aaaaattgga gaaaatcaaa aaaaccatgg aaaatctacc
      481 tgatggagtt tctcttcagg atataatgcc tggtgctaat atggaagata tacaggatca
      541 aatcgaaaga caggaattta tactcaatgg tgagattgaa aaatataata atgcagaaac
      601 ttctgctgaa ttagatatta aatctataga acttcaaata caaaatctga gtattgaact
      661 tgaaaaaaca aagcttgttt caccggtaac aggaaaggtg gtctggataa catctaccaa
      721 accgggtgaa tctatacagg catatactaa tcttataagg atagctgatg taaataggtt
      781 gaagcttaaa tataccgggg ataacatttc ggattttaag cttggcagca aggtagaagt
      841 aaaaattgac gatgcggaat atgagggaga ggttgttatg actccttcaa cagcaccttt
      901 tgatgccgat gaaagtataa aaaggtctat ttttataaat gtaaaaggac ttcctaaagg
      961 tgtttcattg ggttattctg caagaataag ttatcttctt gaatataagg aagacgtaat
     1021 tgtaattcca agagatatgc ttcatggatt tatgggccaa aagacagttt acatactgga
     1081 agatggactt aagaaagata ggtctgttca aacgggaatt gaaactccaa cagaagtgga
     1141 gatcttaaaa ggtcttgaag aaggcgaaga gattatagaa ggttgagggg ggaagattct
     1201 tgctgataat tctaattaga aaaatactta gcaataaatg gatggtccta tgtctcctta
     1261 ttggatcaat tatcactgta gctatggtaa gcagtgtacc tatatataca aatggagtat
     1321 tacagcgaat gctaacccgt gatttagaag gttatcaaaa aaattatggc ggcttccccg
     1381 gaaggtattc cattaaagtc aaattgcatt attatactga taatgtagac gacagagtta
     1441 aggcatataa gtggtttgct gaaagaattg ttgatttgcc tcgacagctt aatgtgcctg
     1501 taattattga taataattct ctaattgctg ataatttaac agcacttcct gaaattcaaa
     1561 gagaggaaaa gccgaaaacc aggttcttta aatttgaggg tatggacgct tttgttgaac
     1621 atgcccaaat cattaatgga cgaatgtttt cagataaaaa agaaggagat attattgaag
     1681 tagttgtaac agaaaaagct tttgattcgc tggaccttcg tcttaatgaa gtttatcatg
     1741 taagtaattt tctaaatgaa gatgaacttt tatgcaaagt caaggtggtt ggaacattta
     1801 caattaaaga tgctgcagat ctttactggt tcgatggaat atggaatttt gatgagagct
     1861 ttttaatgga taataaacta tttagtcaat tgtttataga atctcaaaat cccatattga
     1921 ccgaagttcg ctggaattat gctcttgatt accataaaat taaaatagaa aacataagtc
     1981 atattttatc tgcatatgat acccagttta gatggtttaa aaaatatact gcaatacttg
     2041 atgcaaggtt gccaatcatt ccaatccttg aagaatatca gaagagggaa aaacagctta
     2101 gtttaacatt atgggtgctg caggttccaa tattgctgat gctggcattc tatatgttca
     2161 tggttgcaca gttaatagtc aaaagtgaag aaaatgaaat tgcagttgtg aaaagccggg
     2221 gttccagcag cctccaaata tttaccagct acttgcttga aagtatattg cttggtggaa
     2281 ttgcttttgc tgcgggtcca tccctaggct tatttatatg cagaatattg ggattgtcaa
     2341 acggcttcct ggaatttgta caaagaactt ctctgccggt tgaattgagt aaaactgctt
     2401 acttatactc tctaatagct ttaggattgt ttgttatatc tatgctttta cctgcttatc
     2461 tatcttcgag aaccagcatt gtcctttata aacaaagtaa ggtaagaaaa agtaaaaacc
     2521 caatatggaa gcgttatttt cttgatataa tactgctggc tatatccgga tatggtctat
     2581 atagctataa aatgagaaca cttactcaag tattaacggg tataaagggc acagatattg
     2641 ctatagaccc acttttattc cttatatcta caatgtttat tcttggggta ggacttgtac
     2701 tattaagact attcccttat ctggtaagtt tcattttctg gttgggaaga aaaatatggt
     2761 cacctgcact ttatgcttct ttccttcatg taggaagatc aggaggacaa gaacaatttc
     2821 tcatgttatt tataatcctt gctatttcac taggaatttt taacgccaat gcagccagga
     2881 ctcttaacag aaacattgaa gagaggatca gatatgatat tggagcagac ataaccatca
     2941 aagccaaatg ggaaagtaat gaggtgtctt ttggcggcat gatgggtgca gagcctgaaa
     3001 tggttggaag tgtcggattc ggaagtgata acaaacctac tgaatatatt gaaccgccat
     3061 ttaaacaatt cacggaatta gatggtgttg aacttgccac aaaggttttc aaaaaaagta
     3121 atgttgagat gagaagtata gcgggcagta catataatgt agatctgttg ggaattattc
     3181 ctgatgaatt cggtaaagta tcatggcttc gtccagaatt gatgccatat cattggtatc
     3241 attatttaaa ccttatggct gaggcaccta ctgctttctt ggtatcaagc tctacaagag
     3301 ataaatataa attaaaagag ggagattcca tttacttaac ctggaagggg caaacttatt
     3361 tggaaggcta tatatatgcc tttatagact attggccttc atataatcca aattttaaaa
     3421 accaaaaggg tgaaccaaaa gattttatag tagcaaatta ttcttatatt gcggcaagaa
     3481 tgtccctgga accttatgag gtttggataa aaagagaacc gggtgttact gatactcaag
     3541 tttataatga tattaaagaa aagaagattg ctattacaga cattaaattt acaaaaaatg
     3601 aaattataaa gaaaaagaat gatcctatgc ttcaaggtac aaacggggca ttaacaatgg
     3661 gctttgtaat tactatgctt ataagttcaa ttgggttttt aatatactgg gtactatcta
     3721 tacgcagcag agctcttcag tttggcattt tcagagctat gggtctatca atgaggaata
     3781 ttattactat gcttatttgc gagcaaatac ttatttcagg ggttgccata ttactgggga
     3841 tcataattgg aggtgccaca tgcgacttgt ttattcctct tattcaaata gcttacagtg
     3901 ctgaagaaat ggctcttccg ttcaaggtat ttgcttatgg aggggattat atcaagttat
     3961 atacgattgt agggactatg ctgttgtcag gaattgcaat tttagcaatc cttgtatcca
     4021 ggattaatat taatcaggca attaaacttg gagaggacta gctattaatg ggtagtggga
     4081 gcaggaagca gttaatggca gggagaaagg gaagtaagaa gctaggaaaa gggggtaaaa
     4141 agttaaatgg gtgaggaaat gctggctgtc aaggaaaaaa gcattattcg gcaagctcca
     4201 ataatagtta ctgataatct ggatagagat tttcattcag gaagagaaat agtgcatgcg
     4261 cttaaaaaag ttaacataaa aattgaacca gagaagctta ctattttaag aggaaggtca
     4321 ggttccggca aaacaacttt aataaacctt cttggtggac ttgacacccc tacaaaagga
     4381 aggatattct ttaaagataa agaaataaca agtatgacag tttctaaaag ggatgagctt
     4441 agaaagaaaa atttcggctt tattttccaa tctattgcaa taatatctgt aatgtcggct
     4501 tacgaaaatg ttgagtttgg cctgagaatt gcagggtttg attcgaaaca cagaagagaa
     4561 cgggcggaag aatgccttac cctggtggga ctgggtaaaa ggatgcatca tagagctcat
     4621 gagctttcgg gtggagaaca acagagagtc gccatagcca gggcaattgc ccatcgcccc
     4681 ggtgttattt tcgccgatga gcccactgca gagctagata catatatggg acttcagata
     4741 gtcaaacttt tcaaatccct tgttgagaaa gaaggactaa cagttgtaat gactacacat
     4801 gatccgagca tgatggaaat agcagattgt gtatatactc ttgaggatgg tgaaattgtt
     4861 gatgaacaat gggcatgata gtattaacaa acaggataaa attcaggttg atgttcatga
     4921 aggcactcaa gatgatcaaa gcgaaaatat aataatatgc gaaaacctgg taaaaatcta
     4981 taaaaccaag gaaactgagg ttgttgcttt gcagggcctt gatcttacaa tagaaaaagg
     5041 tgaattaatg gccataatag ggaacagcgg aagtggcaag tccacccttc ttaatatgct
     5101 gggaggcctt gatagacctt ctgcaggaaa acttattatt gatggaaagg atctgcttaa
     5161 atttagtgat aggcaactga tgaaatatag gagagagacc gttggatttg tctggcagaa
     5221 taatgctaga aatctgattc cttatttgac agcaagagaa aatgtagaac tccctctaat
     5281 attaacgggg aaaaagaaaa aggctgcaaa ggcgctggaa ttactggata tggttgggct
     5341 taccaataga gcttacagta aattaagcca gctttcaggt ggagagcagc agcgtgttgc
     5401 aatagctatt gctcttgcca ataaccctaa aattcttctg gctgatgaac ctaccggttc
     5461 tgtagatacc aggacagcag accagatatt ggatgtattc cgtgtaatga gccagacttt
     5521 gggtattact gttattattg ttactcatga caggagattg gcacgtaaag tggacagggt
     5581 agtggcaata agagatggta gaacaagcag tgagataatc agaaaaaggt cttatgcaga
     5641 ggaactggaa ggcatgaagg ttggaatatc aagcggggat gatgatactc acgtggaata
     5701 tgctgtgctg gatagagcgg ggcgtcttca aatccctgcc gaatacattg aagctatgag
     5761 ccttaaaggc agaaataagg tcaaggtatc acttgaagag ggaaaaattg ttcttgaacc
     5821 tccggaaaaa tcctccgatg aaaaagctga tgaaaaagct gttggaaaag ctgttggaaa
     5881 agccgttggc tgaaaaatga aagaactgta aaaggctgtt gaccgaaaaa aatgtttaca
     5941 ttttgcgata aatagggtat tctatataaa gatttcgtaa taatttgcta aaaatatcac
     6001 aaactataat ataaaccaag gaaggagata taattaatat gagtttttat ttggaaattg
     6061 atagcgtata tgcaagagaa attattgatt caagagggaa tcctaccatt gaggtggatg
     6121 ttataactga agacggtaat ttaggtagag cagcagtacc atcaggtgct tcaacaggtg
     6181 cttttgaagc tgttgagtta agagatggag ataaggaaag atatcttgga aagggtgttc
     6241 agaaagcagt tgagaatgtt aataccataa ttgccaatga agttgagggc atgaatgtat
     6301 atgaccaggt tgcaattgat aatttaatga tagcccttga cggaacacac aataaaggta
     6361 gacttggggc aaacgccata ttgggagtat ctctggccgt agcaaaagct gctgcagagt
     6421 ctctgggatt aagcctttat caatacatag gcggtgtaaa ttctaaggta cttcctgtgc
     6481 ctatgatgaa tattattaat ggcggaaaac atgctgataa tagtgttaac attcaggaat
     6541 tcatgataat gcctgtaggt gcatgctgct ttaaaactgc acttcgtatg tgcgccgaag
     6601 tatttcataa cctgaagaag gttttaagtg caaaaggcta tagtacagct gtaggtgatg
     6661 agggaggatt tgctccaaac cttaaaactg atgaagaagc tatacaggtt atacttgaag
     6721 ctgtagaaaa agcaggatac agacctggtg atgatttcag aattgctata gatgctgcag
     6781 ctacagaaat gtatcaggat gatggaacat atctgttctg gaaatccaac ataagaaaga
     6841 caagagaaga aatggtagat ttctgggctg acctggcttc caaatatcca atcatatctc
     6901 ttgaagatgg agttgcagaa gaagactggg agggctggaa actgcttaca gataagatag
     6961 gaagcaagat tcagcttgtt ggagatgacc tgtttgttac caatactgag agattaaaga
     7021 aaggtattga catgggagta gccaactcaa tactaataaa ggtaaatcag attggcacac
     7081 ttactgaaac tcttgatgcg attcaaatgg ccaatagagc cggttataca gcggttgtat
     7141 cccacagatc cggtgagact gaagatgcta caattgcaga tattgcagtt gcaaccaatg
     7201 ccggtcaaat aaagacaggc gcgccatcaa gaaccgaccg tgttgcaaaa tacaatcagc
     7261 tgttaaggat tgaggaagag ctgggcgctt ctgctcagta tcttggattg gatgcatggt
     7321 ttaacctgaa gaataaataa aaacaatcaa atggaaatta aaaaagcgta gagaagggcg
     7381 catattattt ttttgaagaa attattgtat tatatttctt caaaaattca aaatgcgctc
     7441 ttcaaccact tgtattttag gttatattat gttacaattt tataccgtgg gaatgcatat
     7501 ttctccattt tattatttgg aggtgttttt ttgaaaatag gtaccattat actaaacata
     7561 atttatataa ttatttcatt gtccatggtt gtaattgttt tgctgcaatc ggcaaaatca
     7621 gcagggctgt ccggttctat tgcaggtggt gctgaaacat ttttcggtaa gaacaaggga
     7681 cgtacaattg atgcaatgct aagcaaatat actacatttg cttctatagc ttttataata
     7741 ttgtcagtag tactttactt gctattgaaa taatttgtgg agctatttaa tggtttgtaa
     7801 attttacttt aactaaaatg ttatataaaa ataaagctat gtggattaca tagctttatt
     7861 tttgtgttta tgcgtgaatg atatatgaga taattaaaag ggtttgtggt ataataaata
     7921 aaagataatc catgattaga ttatattatg aatgaggtgc cggatggaag aagatagaaa
     7981 aaaacgaata ctggaatata tggaaagtga cacatacaaa cctttccttt ttgaagagct
     8041 tgcaggattc ctggcagttc ctgatacaga actgggtata ttcagaagtg tacttgatga
     8101 gcttgaagca gaagggaagg tatttaagtc aaagaaaaac aggtatggct tgccaaagca
     8161 ttttaactta atagcaggcc gtttgcaaat gaatgagcgg ggctttggtt ttgtaatacc
     8221 tgacaaccgc aataatggta gtaatgacag taatggagat atatacatat ctccggagtt
     8281 tatgcatgga gcaatgaaca atgacagagt gtatgcccgc ataatgtaca aaggttatta
     8341 tgataagaga gctgaaggtg aaatattcag aattattcaa agagcgaatg aaatggtagt
     8401 aggcactttt caaagcagca ggtattttgc ctttgttata cctgacgata agaggattac
     8461 aggtgatgtt ttaataccaa aggatgaagc aggcggtgct gttgacggac agaaggttgt
     8521 cgcacaaata atcagatggc cggaagaatc aaagcacgct gaaggatgca tagtagaaat
     8581 tcttggagat aaaaacagtc cccaggctga tgtattatct ataattagga cgtttaacct
     8641 tccggaaaaa tttcctgaaa acgtaatgca gcaagttgat aaaattggtg ataccgtacc
     8701 tgattccatg ctggaaggaa gaagggattt aagacaatta agaatagtta ctatagatgg
     8761 cgaagatgca aaagatcttg atgatgcagt ttctatagaa aagactgctg aaggctatta
     8821 caggcttgga gtgcatatag ctgatgtcag caattatgtt gttgagggtt ctcctcttga
     8881 tagagaggct ttgatccggg gaaccagtgt ttatcttgct gatagggtta taccaatgct
     8941 cccgcctaaa ttatcaaacg gtatatgcag cttaaaccct ggtgtggaca ggcttacttt
     9001 ttcggtaatt atgacaatca attcttcagg taatgtaata ggccatgaaa tatgtgaaag
     9061 tgttattaaa actactgaga gaatgactta caacaatgta tataagatgc ttgtagaaaa
     9121 tgatcctcaa gtgtgccata agtattccca ccttattgat gattttaaaa ccatggaaga
     9181 gcttgccctt gttttaagaa agaaaagaat agaaaggggt tccatggatt ttgattttgg
     9241 ggaagcaaaa attattcttg atgaggaagg cagaccagtc gatattataa aatttaaatc
     9301 gacaattgct gaaaaaatca ttgaagagtt tatgatagta tgcaacgaaa ctgtagcaga
     9361 acactttttt tggttagaag ctccttttgt atacagaata cacgaggaac cagataaaga
     9421 aaaattgtcc aatttcagca gctttgtccg cattttggga taccgtataa aagggaaagg
     9481 tgaagttcat cctaaggctt tacaggatct tcttgatgaa gtaagaggta aaaaggaaga
     9541 aagacttata agtacaatgc tgcttcgttc acttcagaaa gcaagataca gtgaacaaaa
     9601 tgcgggccat tttgggcttg catcaaagta ttattgtcat tttactgctc ctatacgaag
     9661 atacccagac cttttaatcc ataaaattat gaaacagatt ctaaaaggcg aaatgagcaa
     9721 ggaaaaaaca gaatactatg ataagcatct tcaggaactg gcaaggatgt gttctgaaag
     9781 agaaagggct gcagatgatg cagagcaggc ggtagatgat cttaaaaagg ctgaatacat
     9841 ggagcagttt attggtgaga cctttgaagg cattattaca ggaattacta acttcggcat
     9901 gtttatagaa ctggacaata ctgttgaagg ggttgtaaga ctgtcaagca tgtatgatga
     9961 ttattattat cttgatgaca tgagatatct tctcctgggt gagaagacag gaaaacagta
    10021 tagaataggg gatgctataa aggtaaaggt tgtaaaaact gataaaattg cccgccaaat
    10081 agaatttgag ctggtgaatg agcctaataa aaaacattcc agtaagaaaa gatcacgaaa
    10141 aaacgagtca gagtgaacaa ttgcttatcg ggtgcaatta aaattttgaa gaaatgtatt
    10201 acagaagcta tgattacggt tattagagcc ttcactgatg tttcttccaa taatcttttc
    10261 taatttcttc aaaaataaaa tatgcaccct ggcttatctc atgatttcgg gaaatgaaca
    10321 accagatatg cctctgcttc agattcagta atattttcta tagtatgttc acgatctccc
    10381 ttatatcgtg ctgtatcacc ttcacataga ataaccgaat aatctcctga tgtaaccctg
    10441 aactttccag aaataactgt aaaaaattct tcagcattag gaaaatgcgg atttgatttg
    10501 tttttcccaa gagctttaaa agtagtgtgg tatacttcaa ggttatctgt catatgcaca
    10561 ggtgaattaa ttctgaattt aactgaacgg tcatctgaat aaataacagg tgcatcgtcc
    10621 cttctgataa cttcaatagg cgaattattg ttactctcca ttagttctga aattgaaaca
    10681 tctaatgaac gggctatttt ccaaaccgtt acaacagtag gatttgcttt ttcctgttct
    10741 atttgcgaga gcatggattt tgaaactccg gacctttttg aaagctcgtc caaactcata
    10801 tttttttgtt tcctcaaaat cgttatattc ttgccgattg gcggcggttc aattgcaatc
    10861 atttttataa tactccttat attttcctta tattttctga tagcatatag gaaagtataa
    10921 atttcctggt atcctagtta aaagtctatt gacatacttt cacttgttca atataatgaa
    10981 catagagtat aatataataa acattaaata taatcaatta tatgttacac aaaatttagc
    11041 tacatatata ttatgtatat atataacgca aaaataatta tacaaggaac aagttggttt
    11101 ttttgcgtat taattaaggt aatataagcg taaaaacttt tatagcgtag ttccttttgt
    11161 gtgttatcta tagtatataa ttatgcgtta attcttgcaa tacctaaaaa tggcagtgag
    11221 agctatatac aaagtagaaa gaaggaatga ttatgactat gatgaaagtc gtaaaaaaag
    11281 aagaacctgg gaagggggta agtataacta cagcatcagt acctgaacca ggacctgagg
    11341 aagtactgat aaaggtgaaa ctggcaggta tatgcgggac ggatttacat atatataaat
    11401 gggatgaatg gtcaaaaagc cgcttgaagc ttccaattat aataggtcat gagtttgttg
    11461 gtgaaattgt tgaggtcggg tcaaatgtaa aacatctaaa aaagggacaa agggtttctg
    11521 cagaaggaca tataacctgt gggtattgtc ggtattgcca gaatggaaaa ggtcatatat
    11581 gtcaggacgt aaaaattatt ggtgtggata cgaacggttg ctttgctgaa tttataacta
    11641 tgcccgcagg aaatgtatgg cctgtacatg atgcaatacc tgacaggtat gcagctttat
    11701 ttgatcccat aggcaatgct atgcatacgg taatgtccca acccgtatca atgaagactg
    11761 ttcttataat gggagcagga tcaataggcc tttttgcaat tcctattgca aaagttaacg
    11821 gtgcggaaaa agtaatagtg gttgaaccca acccatataa aagagacctg gctgtcaaat
    11881 caggtgctga tcttgttatt gattcaaata aaactgatat tgactcagaa ataatgagtt
    11941 ttacgggcaa taccgggcct gatgtttttc ttgaaatgag tggtaatccc ggagcgttgc
    12001 gcaagggtct ggatttatta agtaaagggg gaacagccag tttgttaggc atacctgaaa
    12061 gagaagtacc tataaatatt gctgatgaaa taatatttaa gggaattacc atacatggta
    12121 taaccggcag gagaatgttc gaaacatggt atcagtgtca gagcttttta ctaaaggatg
    12181 ggaagacaat agaacctata attacacata ccttaagcat agataatatt gaggatggct
    12241 ttcatcttat ggagaacaac ctagctgcaa aagtgcttgt agatattgag ttataataaa
    12301 tataggagga tgaattacta tggctaaaaa gttttataac aagcttgatg aagagcttga
    12361 ccaattaaag aatgcaggta catataagag tctcaagtat ctaaaatcat ctctttcagg
    12421 cagagcagaa attgaaggct ttggcgatgt aataatttta tgctcaaata actatctagg
    12481 tctcgctgat aaaccggaaa taatacatgc gggtgttgaa accttgaaaa aatatggcgc
    12541 aggggctgcc agtgtaaggt ttatctgtgg tacatatgat atccaccggg agcttgaagg
    12601 aaaaatagca caatttctcg gtactgagtc agcattaaca tatacatcat gctgggccgc
    12661 aaataccgca gttattcctg ctctgctaaa gtccggtgat tgtgtaatat ctgatgaact
    12721 gaatcatgcc agtataattg atggctgcag gatggtgggc agagaagtaa aaagatttgt
    12781 ttacaaacat tcggacatga aatctctgga agaaaagcta aaagaatcag agggaacagg
    12841 cacaaaactg attgttacag atggagtatt ttccatggag ggagatatag ctaatttacc
    12901 tgatattgtt gaaatagcag aaaaatatgg agcaattgta atggttgatg attcacatgc
    12961 aacaggtgta attggtaaaa cgggaagagg aactgaagaa tattataata tgattggtaa
    13021 ggtagaccta ataacaggaa cactgggaaa agcactggga ggtgcagggg gcggatttgt
    13081 ggctgcagga aaagctatta ccgatatatg tatacagcgg tcaagacccc atttattttc
    13141 aaattctctt cctcctgtaa tggcttccat aggtattgca gctataaact atcttgatgt
    13201 aaatccatca attgtagatt cactgcataa aaagacggcc tatttgcgta atagcttgaa
    13261 acaggcagga cttaaacctc ttgaaggcga tagtgcaatt gttcccataa tattaggaga
    13321 tacagcaaag gccataagag ttgcagaaaa tatgttaaag aagggtgttt atgctgtagg
    13381 atttggatat cctgtagtac ctgaaggaac agcaaggata aggatacagg tgtcagacgc
    13441 ccttacctat gaagacattg atattgctgt gagggtaata agagaggctt tattatgaat
    13501 tatggatcag gaagaaaatg cgcccataca ataatgaacc tctggctttt tttctgaaat
    13561 tggtataaaa tctaaatgta aaatctaata taaaagctta taaaaaatat aaggaaatat
    13621 agggaaatag gcaggtgaaa taaattgaag aagacaccac tatatgaaca gcatgtaaat
    13681 ctccgtggca agattgttga gtttgccggg tgggctttac ctgttcagta ttcgagtatt
    13741 cttgaagaac acgagtatgt aaggaattcc gcagggctgt ttgatgtatc tcatatgggg
    13801 gaaatcatca tcgaaggtcc ggacgcagaa aaattaattc agaaattaat taccaataat
    13861 atagccaaca tggagcaggg gaaaataata tattctccaa tgtgttatcc agatggtggt
    13921 acagttgacg atatattggt ttacaaaatg tcaaatgaag aatattttct ggtagttaat
    13981 gcttcaaaca ctgataaaga ctatcaatgg atgctggata atgctgaagg tgacgctgaa
    14041 ataactaatg tttccagtaa ttatgcacag cttgctcttc aggggccaaa agccatggag
    14101 atattaggaa gacttgcaaa tttcaatctt gaaaagataa gacctttccg gtttgaagca
    14161 aaagtagata ttgccggttt tgaagcacta gtgtcaagga caggctatac aggtgaagat
    14221 gggtttgaaa tatacatagc acctgaaaat gcagagaagc tctggaataa aatactagat
    14281 gagggtaaag aagacaggct tgctcctgca ggtcttgggg caagagatac tcttaggttt
    14341 gaagcagggt tgccccttta tgggcaggaa atatcaaagg agattactcc tgttgaggca
    14401 ggtcttagca ggtttgtaaa gtttgataaa ggtgatttta taggtcgaag tgcattgctt
    14461 gcccagaagg aaaacgagcc cgggaggaaa ctggttggct ttgaaatgat tgagagaggt
    14521 attccaagga gtcattatga agtacagtct gaagggaaaa gtataggatt tgttacttct
    14581 ggaaatttct cgccaacatt gaagaaaaac cttggcctgg ctcttatatc acaggaatat
    14641 tcagatttag gggaagttat agaaatagtt attagaaaca agactttaaa ggctacaatt
    14701 atagaaacac cattttattc aagaaagcga ggatgaactt atgaaactgg aaagcggatt
    14761 aaagtattca aaggaacatg aatgggtaag agtagcaggt aataaggctt atataggaat
    14821 tacggatttt gcacaaaact ctctggggga tattgttttt gtagaaatgc ctgaagttgg
    14881 agcagaattg gcttcagggg acactctagg tgttgtggaa tctgttaaag cagcttctga
    14941 catatatact cccgtatccg ggactgtaac caagataaat gaaaatctta acaacagccc
    15001 ggaaagtata aatgaggatc cttatgaatc ctggataatt gctcttgaat tgtcagaccc
    15061 ggcagaactt gatgatctca tggatgaaaa ggagtatgaa caattctgtt caaaagaggg
    15121 ttaagtttaa agattgttag ccataaggaa taattacaaa aaggaatgat tatttatgag
    15181 aaattataca tcaaggtata ttcccaatac agaagagcag caaaagagta tgcttgaaac
    15241 aatgggaatg aaatccttgg atgagatatt caaggatata cctgaagagc ttaaattgaa
    15301 tagagcattg aatattcctg atgatatggc agaatatgaa ctcataaagc atatgcaaaa
    15361 tatggcatcc aaaaatgcaa atatggatga ttatgtatgc tttcttggag ccggagctta
    15421 cgatcattat attccaagca taataagaca tatgcttatg agaggagaat tttatactgc
    15481 atatactcct tatcaacctg aaataagtca gggaactctt caagccatat ttgagtatca
    15541 aacaatgatt tgcgagctta cagggatgga tgttgcaaac gcttctatgt atgatggagc
    15601 ttctgcagct accgaggcaa ttattcttgc ctgcaatggg aaaaggaaaa atgaagttgt
    15661 aatttccgct gcactaaatc ctgaatacaa aaaagttatt aatacgtatg cccggtttaa
    15721 tgggtttaaa gtgatagaag cagcatattg caatggaata accgatatat ccgacctgga
    15781 gaaaaaaata acaggggata cagctgctgt atttatccag agccccaatt tttttggtgc
    15841 aattgaagat ctgaccaatg caggccaaat tgctcataag aatgacattt attttattgt
    15901 gtctgtcgac cccatttcac ttggaatcct aaaaccacca gcagaatatg gggcccatat
    15961 tgttgtgggt gagggtcagg cactgggtaa ccctatggga tttggagggc cttaccttgg
    16021 gttctttgct gtaaaaaagg aactaatgag aaaaatgccg ggaaggattg tagggcagac
    16081 aaccgatgta aatggtaaaa gaggttttgt ccttactatg cagacaaggg aacagcatat
    16141 aagaagggaa aaggctacat ccaatatttg tacaaatcag gcattgaatg ctttaacagc
    16201 taccatatat ttaactgtac ttggcaagaa aggtttaaaa gaagtaggag aactttgtgt
    16261 tcaaaaatcc cattatgcat atgacagact acttgaaaca ggtaagtttg aagctgcttt
    16321 tacatctcct ttctttaaag agtttgcttt aaaagctaaa aatggaattt caatagaaga
    16381 actaaacatg aaacttttaa atgacaagat aatcggtgga tatgacctgg gcaaagactt
    16441 tgaggagatg aaagacggat ggcttgtggc cgtaaccgaa aagagatcaa aggaagatat
    16501 tgataggttg gtagagaaag tcaactgctt gtaagcaaat ttaaatttgt ataataaaga
    16561 aaatagaggt tagtttgaag gaaattggta aatttctgaa aaattaatgc acatgtgcat
    16621 attttaagca cagtaatatt tacaaaaaat agattgcaca tggcaggaaa ggagcttgaa
    16681 atgaatgaca gagctttaat ttttgaaatc agcaagccgg ggcgtatggc atattctttt
    16741 gctgactgcg atgttcctga aaaaaacatt gaacagttaa ttccacggga gtatatgcgt
    16801 gaaaatgctc ctgagcttcc tgaagttagt gaagtagaag ctgtacgtca ttttgtgggt
    16861 ttgtcaagac taaaccatgc agtagataca ggattttacc ctctgggatc atgtactatg
    16921 aagtacaatc ctaaagtaaa tgaggatgtt gcagctttaa atggtttttc ttcagtacac
    16981 cctttgcagc ctgaaaatac agtacagggg tgtcttgaac tagtatacag aacaggggaa
    17041 atgttggccg aaataacagg aatggaaaaa gtatcacttc aacctgccgc cggagcacat
    17101 ggtgaattga caggacttat gataatgaag gcatggcata ttcacaacgg tcaggagcaa
    17161 aggagaaaaa ttattattcc tgattcagcc catggaacaa accctgcatc tgcagctttc
    17221 gcagggtttg aggttataca ggtcaaatct aatgaaagag gaggtgttga cctggattcc
    17281 ttaaaatcaa taatgggtgc tgatattgca ggccttatgc taaccaatcc caatactctg
    17341 gggctttttg atgaaaatat aaaacagatt gcccatatag tgcatgaggc aggaggtctc
    17401 ctatactatg atggagctaa tgcaaatgct attttaggaa taacaaggcc tggagatatg
    17461 ggatttgata tagtgcacct gaatcttcac aaaactttca gcacacctca cggaggagga
    17521 ggacctggtt caggacctgt tggagtaaca gccaggcttg tacctttcct tccctcgccg
    17581 atagtggagt ttagagacgg aaaatatcgc ttcgattata actgtcctct atctataggt
    17641 aaggtaaagt ctttctacgg aaattttgca gttgtagtta agacatatgc atatatatta
    17701 tcaatgggtc ctgaaggcct taagatggcg tcggagacag cagtcttaaa tgcaaattat
    17761 gtaatgaaca agcttaagga atattatgaa ctcccatatg agcgcagctg tatgcacgaa
    17821 tttgttattt cagcacaaaa gcaaaaaaat ctaggggtat cagcccttga tgttgcaaaa
    17881 aggcttatgg attatggaat acatccaccg actatatact tcccgctaat agttaaagaa
    17941 gcattaatgg ttgagcctac agagactgag agcagagaaa cccttgatga atttgctgaa
    18001 gttatgatta aaatcgcgga agaagccgag cacgatcctg aaacagtaaa aaatgctccc
    18061 tacagcacgc cggtaggacg tctggatgag gtcagggctg caagagaacc tgtattgcgt
    18121 tgggacaaag aaaaatagag cagggcgggt gcatttttct atatttgaag aaaagtatag
    18181 caagagttca tttccacgaa taatattcac tttagaaacg aaaattctcc ttctgcttta
    18241 acttacagaa ggagaatttt tatacatggt taaactgcga actttttgat ttcatcacag
    18301 aaaccttgac aatcatcgac gtgtgcctct actttaatcg gtttgctaag atccagacta
    18361 aagataccca taattgattt tgcatcaacc acataacgtc ctgatgaaag atcaacatca
    18421 aaatcgtact tgtttacaat gttgacaaaa tcttttacat cattaataga ttttaacatg
    18481 atattgaatg atttcactag acatccccct ttttattggt acttaaattt aatacttgag
    18541 tgtccataaa taaacatctg caattaaaca tctgtaatta taataccctt tctgatagta
    18601 taagtcaatt aaattattgt taaactttcc agtttaaatg tcatttaatt gactctataa
    18661 aatatgtaca ttataattat tagggtaata tgtggttaaa atagttaaag atatattata
    18721 gataattggg agatgttatg aaaaaagttg cattttgcac tcttggctgt aaagtcaatc
    18781 aatatgaatc ggaagctgtt tcaaacctgt tcgaaaatgc tggttatact attgtagatt
    18841 tcgatgaaat agcagatata tatgtaataa atacctgtac agttacaggt attagtgcga
    18901 gaaagtcaag gcagatggtc aggagagcca aaagccttaa ccgggaagca attattgttg
    18961 ttatgggatg ttattcacaa acttcccctg aggaagttag caatattccg ggagtagatt
    19021 taataattgg caccagtgat agaaacaaaa taattgaata tgtggaggaa attacgcagt
    19081 gtggtaagca attgaatact gttgagggca taatgaagaa aagagacttt gaagagctgc
    19141 atgttgacaa atacaaggaa cgcaccagag cttatttgaa gatacaggaa ggctgtaccc
    19201 agttttgctc ctactgtata attccgtatg caagaggacc tgtaaggagc cggaatcctg
    19261 aagaagtact caaagaggtt aaaaggcttt cagataacgg ttttaaagaa gttattttaa
    19321 caggtataca tgtagcatcc tatggaagag atttgaggaa tacctcatta ttggacatta
    19381 tttgcagaat acatgaagtt gatgggattg agagaataag actgagttct attgaaccta
    19441 caaccattac taatgatttt gtgcaaattg taaaaaacct taataaactt tgccctcatt
    19501 accatatttc tctacaaagt ggatgtgatg aaaccctaaa aagaatgaac agaaaatatg
    19561 acacatctga atatagaagt gtagttgaaa gactaaggga caatatcaat gatgttgcta
    19621 taacaacaga tgttatggta ggatttccag gggaatctga taacgaattt gaacaaactt
    19681 atgaattttt acaggaaata tgtttttcga aaatgcatgt tttcaaattt tcgcctcgta
    19741 aaggaacacc tgcttattct tttacgaatc aagtacctgg agatgtgaag gaagaaagaa
    19801 gcagaaagct aatcgagctt tctgatgaat gtgccctgaa attccaaagg cagtttgaag
    19861 gaagaacaat gcccgtgctt tttgagcagg aagcaggtga aaatagtatt gaaggactga
    19921 ctccaaatta tatacgtgtc ttctgcagag gaagcaacag cttaaaggga gagattaggg
    19981 atgttgtact taaaaatacc tataatgatt ttatggacgg ggaaataatt aacccgtaac
    20041 agtagccctt cgtaaatatt tctacaaaaa tttcaaatgc acctccctgt attccttttc
    20101 tccttgcata aatcgctttg atatattaat attatatata tactgataat attggtatcg
    20161 ggtggtaaaa taacaataca gcttttatat tggagcgtga taattatggt taacaatgaa
    20221 acaatgatgt tcaatgtaga taaagacaat gctgatgaaa ctcatgagat actcatgaca
    20281 atatatcagg cattaaagga aagaggttat aatcctataa accagattgt gggatatatt
    20341 ctttcaggag atcctactta tataacaaac cataagaatg caagaagtct tgtcagaaaa
    20401 cttgaaagag atgagctgct ggaagaagtt ttgaaatttt atcttgaatc caataagaac
    20461 agataatttt ttcaagggtt tttattataa tttgcaccaa ttaagatcct gcttaagagg
    20521 caggagatct tttggtgctt tggctaaacc gggaatattg tgttacccgg tttttgtgtt
    20581 ggaggagtat atggaatata taaaattagg gaatacagga atagaagtgt caagaatgtg
    20641 ttttggtgct cttattattg gccctttgca ggttaacctg cctgtagaca ggggtgcgga
    20701 aataattaag agaggtcttg agctcggtat taatttcata gatactgcag agctttacgg
    20761 tacttaccct catataaggg aggctgtaaa aaggtcggga aaaaggcctg ttattgctac
    20821 aaaatgctat gcttatacaa aagaaggggc tgctgaaagt cttgaaaaag caagaagaga
    20881 attggacatg gatgtaatag atatgtttct tcttcatgag caggaaagca gactgacact
    20941 taaaggtcat aaagaagctc tcgattactt tatatctgca aaggaaaaag gaatcattag
    21001 ggctgttggt gtatctacac ataatataga agtagttgaa gcatgttctg aaatgcctga
    21061 aatagatgtt atacatcccc ttgtaaatat taaaggtatt ggaattggtg acggtacaat
    21121 tgatgaaatg ctgaaagctg ttaaaaaagc ctatggtgcc ggcaaaggca tatatagcat
    21181 gaaacccctt ggtgggggaa atcttcttgg atcttttgag gattgtatta aatttgtgtt
    21241 gaatattcct tatatacatt caatagctat tggaatgcaa tcggaggaag aagtagaaat
    21301 taatgttggt atttttgaaa gtcatgaaat acctgaagaa ctaaaagcaa gaataaggaa
    21361 aattaacaaa aaacttcata ttgattattg gtgtgaaggg tgcggcaaat gtgtagagcg
    21421 atgtaaacaa ggtgcattgg aaattgttga ctccaaggca aaagtcaatt atgaaaaatg
    21481 tgtattgtgt ggttattgtg gaagtgtctg cccgcaattt gcgattaaaa tatgttagca
    21541 ctttatttcc tgagcaaagt gagtttgtta ataacatgaa gaaaacattg gaggtgaatc
    21601 cgtgagaata atgggaatag attatggaga tgccagaata ggcatcgcaa tcagtgatcc
    21661 ttttggctgg actgcgcagg gtctggatac aatagattgg aaaaatgata ttaattatcc
    21721 cataggaaag attattaata tcattaaaga atatggtata gaaaaagtaa tagtgggata
    21781 ccctgtaaat atgaatggca cgttaggtga aagggcactt aaaactgaag aatttattaa
    21841 gcaactagtc ataaggactg gaaatattga tataataaaa tgggatgaaa ggctgacaac
    21901 tgttgcagca catagaatac tcagtgaagc tggtgtaaaa gcgtcaaaga gaaaaaaatg
    21961 tgttgaccgt atagctgcta tttatatatt acagggatat ctggacagtc tccgaaataa
    22021 ttattcgaaa aataccggtg ataccgagat gcagtaaagt tataaaaaaa tttacggaaa
    22081 atataggaaa gcttttgata ttatgctatg ctataataaa tacctattaa aattaaggag
    22141 ggaatctaat gcctgaagaa agagatgata ttgtagtact ggttgatgaa aatggggaag
    22201 aggaagaatt cgaattcgtt gatactgtcg aaatggatgg caatgaatat gtaattcttt
    22261 cccctgtaag tgaagatgaa aacgataatg atgattttga tgaagaagta gttatactaa
    22321 aagtagacaa aaaagaagat ggagaagaat cttttgttac tgtagaaaat gaagacgagc
    22381 ttgatgccgt gttcgaagag tttaaaatga gaatagaaga tgattattac ttcgattttg
    22441 atgaagaaga cgaagatgat gaagatgaag aagaagatga tgatgatgat gatgaattat
    22501 aactttcata cttgtattga tgaatgaaac aagggataaa caatagaaac tgcttaagtt
    22561 caataacagc cgggatataa aatgcccagg ctgtttttta atttttttta ctaatttctt
    22621 tgctatgtag agggatttcg tgtattatag tagaataata tatatttatt cacgtttttt
    22681 atactgaaat cagagtttat gtagtagaaa atttacgata gaaaataatt acgggggttt
    22741 ttacacaaat gaaagaattt tttactagaa caacatctat acttcagaaa aggaaaatag
    22801 ctgttgcttc aattgcagtt gttattttac ttattttttc tcttattgtt gtaatagttt
    22861 ctcataacaa caggcaatca gacgaaaaca ctgctaaaca gcccgaagaa agtgaatcta
    22921 ataattttca agacaatgaa caagatatgg ccgaaaataa taactcaaaa gatgaatcct
    22981 ttgcattccc catcagtggg gtaaggcctt atgctgtaat gatagataat gagggaacaa
    23041 aatgtctgcc gcaaggtggg cttcacaagg ctcaggttat ttatgaaata atagttgagg
    23101 gtggtgaaac cagatttatg ccagtctttt gggcatcact cccttctgag gacagcaagg
    23161 agagcaaggt cacacagcag gaaacccagg gtacaaagga aacaaatggc acccaggagg
    23221 ctaaggacat tcaaaacatt caaaatgcca gattcccgga acttgaatta ataggaccgg
    23281 taaggagctc aaggcattat ttccttgact atgtacttga acttgatgct atatatgtac
    23341 attttggatg gagcccaaga gctatgagtg atattccaaa actcaaaata aataatataa
    23401 atggtgttgc caatggagga gaaatatttt gggatttaac aaaagataga aataattggc
    23461 aggattcata tacctctatg caaaaaatta atgagtatgt aaagaaggtg aaatacagaa
    23521 ctgagactga taaaaagtcc gtattcagct ataataaaga agatgtggaa attcctggcg
    23581 gcgttgatgc aaaaagtgtt ttaattaaat ataaccaggc taatacaagc caatacttat
    23641 ataatgcaga aacacgtgaa tatagccgcc tgagaaaagg caaacctcat atggaaagag
    23701 caactgagga gcaactaaag acaaaaaata taatcattca attcacaaaa aattacacca
    23761 taaaaggtga tactgaggat cgtcaggaag ttgaaaccac aggaaacggc gaaggttggt
    23821 atattacctg tggaaaagca gtaaaggtta aatggactaa aaaatcaaga aatgaagcaa
    23881 ctgaatataa atatgaaagt ggagaaccgg ttcttttaaa tcctggacag acatgggttc
    23941 aaatagtacc tatatatgga aatgtagaaa taaaagggga gttgtaaaag atggccattc
    24001 atgatgaagt aaaaaaaata gcagatgata tttatgctaa gcttattgac attcgaaggg
    24061 gaattcataa atttcctgaa ttgggtttga aaacatatag aacagctgaa cttattacag
    24121 gtcacttaac cgggctggga gtggaagtta caaaagcggc agatggctca ggggtggttg
    24181 gattaataag gggtaaggca ggttcaaaaa ccgtagctct aagagctgat attgatgcct
    24241 tgcctgttaa tgaagagaat tcttttgagt atagatcctg tatatcaggt gctatgcatg
    24301 cctgcggtca tgattttcat acagcatgcc tattgggggc ttctgagatt ttataccggt
    24361 taaaagataa aataaatgga aacataaagc tcttatttca gcctggtgaa gaaggacttg
    24421 atggagcact ggcaatgatt gaggaagatg tccttgaaaa tccacatgtt aatgcttgta
    24481 ttgcagcaca tgtttggcca tatgtaccgg tagggaaaat tgctgttata tatggacctt
    24541 cattttcggc tattaataat tttataataa cgattaccgg caaaggcggg catggtgcaa
    24601 tgcctcatga taccgtagat cctataatag caggatgtaa tataataaat gcgcttcaaa
    24661 caataaaaag ccgaagaatt gattccttcg aaccggcaac agtttccata tgcagcttta
    24721 atgctggtac aagtgtaaat ataattcctg ataccgctgt tataggtggg acattaagaa
    24781 cttttaatct ggacttaaga aaaaagattg aaacaatgat ttgtgaaata tcaaagggaa
    24841 ttgcagaacc tatgggagca aaagtaagtg tacagtttat gaatgaagca cctccggtca
    24901 tgaatgacag ggaaataact gaattattca taaattcagc atccaaaatt gcagggaaag
    24961 agaatattat tacagatata agacctgcaa tggtaagtga agattttgcc tttttctctg
    25021 agaaagtacc ttctacatat ttctggcttg ggtgcagaaa cgaagaaaag ggtatgatac
    25081 acccacttca tagttctaat ttccagggag atgaagagtg tattaaaatt ggtgccgcag
    25141 catttgccca attcgcaatc gattatctgg ctatttgact atcttataca ccattgattt
    25201 ccaccgtcaa ttataataca attgcatagt ataaatatgc cataattata ttatatatcc
    25261 atataaagct taaagggggc gttgatattg aaggaaaagc gtaaacatgg actcaaaata
    25321 tgctggaagc aagaggaaca gactcttgaa tttgcagccc gagagttggg cagatatgca
    25381 gaaatgatga tggaaaataa tctggatgtt acatattcta aaagttgctc ctgttgtgaa
    25441 cctgagaaag aggcaattag tctgggcctt tttgaaaatt ttggcataac ttcaaaagaa
    25501 gttgaaaata ctgcgtttga tgacgaaata catattgata ttgaaaatgg caagggaatc
    25561 attgcaggaa ttaaccccag aagtgtactt cttggagtct accgttttct tactgaagca
    25621 ggttgtatgt gggtaaggcc cggaaaagat ggtgagttca tacctgcaaa aaatattttc
    25681 aatatttcgg ttaagcttca tgaaaggcct tcatacaggc atagaggaat atgtattgag
    25741 ggtgcaaaca gctatgaaaa catagagcag ataattgatt gggctccaaa agccggcttt
    25801 aacgcctact ttttccaatt cagagaagcc tacaccttct ttgaacgctg gtattctcat
    25861 acaaacaatc ccttaaagaa gcctgaggaa tttacagtgg aaatggcaag agagtttttg
    25921 aaaagagcag agaaagagat tgaaaagagg ggaataatat accatgcagt agggcatggg
    25981 tggacctgtg aaccttttgg gataccaggc ttaagctggg ataaggctga ttatattgta
    26041 aaacccgaag ttgagaaata tctggctgaa gtaaatggga aacgtgaatt gtggggtggt
    26101 atacctctta acacaaactt atgttattca aacactgaag taagaaatac gattacaggt
    26161 gcaatcgctg attatcttga agaaaataca actattgata ttctccattt ctggctggca
    26221 gatggcagca ataatcaatg cgaatgcgaa aactgcagaa aaaaactgcc atcagattat
    26281 tatgtgatga tgctgaatga acttgatgaa atacttgctg caagaggcat agatacaaaa
    26341 attgtatttt taatttatgt tgacctgcta tgggcaccgg aaacggaaaa aatcaagaat
    26401 cccgacagat ttattcttat gtttgcccct attacaagga cttacagcat gccttttgaa
    26461 accgacgaag aattacctga gcctccagcc tataaacgaa atcagctgaa atttgcatcc
    26521 agtgtcaaag aaaatctcgc tttccttaaa tcttgggaaa aggcttttaa aggagacagc
    26581 tttgactttg attatcattt tatgtgggat cattatgctg atccggggta ttataaaata
    26641 gctgaaatat tgtataaaga tattaataac ctaaaaggaa ttggtctgaa tggttatgta
    26701 agctgccaaa cccagagagc atttttccct acgggtttgg gaatgtacat aatggggaga
    26761 acattatggg atagcagcct ggattttaac gtaattaggg atagctattt ccaacgtgct
    26821 tttggaatag actgggaaaa atgcagagat tatatggtcg atctttctaa gctttttgat
    26881 cccccctatt tgaggaagga aaaggagata gtcagtagtg aagcagctga aagattttca
    26941 ggcatatgta catttgtaga tgaattcagg aaagtaattg actcaaatgt atccagtgaa
    27001 aatccatgtc atgcagcatc ctggaattac ttgaaacatc attccgattt atgttgtatt
    27061 ctggcattag ttttagaagc aagagcactg ggtaacaatg caaaagcgtc tgctatttgg
    27121 aaactggctg aaaaatatgt ccaggaacat gaagataact ttcagactgt tttggatgta
    27181 tatttatttg tttcaaccat taagggtaga cttggattca gcaaataaat catagaaacc
    27241 ttcccaaatt gacaaaaaag tattaatata tatagtatag tataagttaa tataacatag
    27301 actaaaggtt tttggaggta cagcaatgaa agatttaagg aatatgagtg tagatgaact
    27361 aaataatatg aaggaaaaac tgcaaaaaag atacgatgat tttaaaaaac agaacatgaa
    27421 actagatatg tcaaggggta agccgggtgc agatcaattg gagctttcca tgggtttgtt
    27481 tgaatatgca gatcatgagc attatagagc ttctgatggt acagactgca gaaattacgg
    27541 cggacttgac ggtttacctg aaatgaagaa gattttcgca gatgtacttg atgtaactcc
    27601 cgctgaagtt attgtaggag gcaatgcaag tcttaattta atgtatgatc taatttctca
    27661 agctatgact catggtgttg caggcagtga agttccatgg ggtaagttgc cggctgttaa
    27721 gtttttatgt ccaagtcctg gctatgaccg tcatttcgca atatgcgaat attttaatat
    27781 cgaaatgatt attgtcccaa tgaaaagtga cggacctgat atggatatgg tggaaaaact
    27841 tgcaactgaa gatgaatata taaaaggtat ctggtgtgta cccaaatata gtaacccgga
    27901 tggcatcaca tactctgatg aagtagttga acgttttgca agaatgaaaa caaaaagcag
    27961 tgattttcgt atattctggg ataatgccta tgttatacac catctatatg atcagcatga
    28021 tgaattgaag aatattctta aagcatgcaa ggaagctggc aatcctgaca gagtattcat
    28081 gtttacctca acctcaaaaa ttaccttccc gggtgcaggt atttctgcaa tagcatcaag
    28141 tgaaaacaat atagcagaaa ttaaaaaacg catgaccaga caaactatag ggcctgataa
    28201 gcttaaccaa ctgaggcatg tagctgttct gaaggattta aataacataa atgaacatat
    28261 gaaaaaacat gctactattt taagaccgaa atttgatgtg gtacttgata gtctggagaa
    28321 ggaactgggt gaaaagaata ttgcacaatg gaataaacca aggggcggct attttgtgag
    28381 ccttaacgtt atgaacggat gcgctaaaaa agtggtggca aaagctgctg aggctggtgt
    28441 aaagcttact tcggcaggag cgactttccc atatggcaaa gatccaaatg acagcaatat
    28501 tagaattgca ccttcatttc catcattatc tgaattggaa aaggctatag aattattatg
    28561 catttgcgtt caactggtaa gcattgaaaa aatactggaa tagtctggaa tagtttggaa
    28621 catatgaaat cgaaaggcaa atgtaagcgg cagaaaccgt tttcatttgc cttcccattt
    28681 gctgtattcc ttattttaga ttctgaagcg ctttcaaatc agaatttttc cccataacaa
    28741 caagaatatc tccttcatca aactttgcat ctgcgttagg taatacattc agattttgaa
    28801 ttttccttaa agcgataata tttaccccaa attttgatct tacagaaaga tctcttatag
    28861 tttttcctat ccaggtagag gggacagtta tttccattat actatattcc gggaaaagct
    28921 ccatcacatc tataatattt ctgtataata tgctgtttgc cgcccttatt cccatatctt
    28981 tttccgggaa aacaactttg tccacaccta ctttatataa tacttttgca tgaagttcat
    29041 ttgatgcttt tgcaagtaca tattttgcac ctaattcttt taataaaaca gttgttaata
    29101 tacttgattg aatatctcca attgctacaa taacagcatc aaagttttta atccctacag
    29161 atttgagaaa ttcttcgttt gatgattctg caacaatagc atgggttact tcgctggaaa
    29221 actgctgtac aatatcttca tttttatcta cagccaggat atcatgacca tgtttggaaa
    29281 gagtttttgc taaactttcg ccaaacctgc caagtcctaa aactagatat gatttcatat
    29341 taactttctt cctcctctat ctattcatac cttcatttat ttatttactg attggctaat
    29401 caactaacca acttaccaac ccaatacaca ccacacacca cacaccacac accactcact
    29461 atccactacc cgctacccaa tccccactat ccaccatcca ctacacacta cccaccactc
    29521 actatctacc atccaccacc cactaccttc ccctttatcc tacacttatt ttgccttcgg
    29581 gatatttata tggaagtatc tttgaaccct gaccaaagga gataatcatg gcagccgata
    29641 aaggacctac tctaccaagg aacatagtga gtattaactg cagcttgctt atgaccttaa
    29701 gtccaggtgt tatacctgta gtaagtccta ctgttccgaa gccggaaaca gcttcaaata
    29761 atgcctccat tagagtacct tctttattta tcaaaagtat tatggtagtt gcaaatacta
    29821 aagcggaact taatgtaacg atggctaagg acttcctaag aacagtatcc ggaatttttc
    29881 tacttaatag attaacatca tgttccccct taataaatga caatgtagca aaaattataa
    29941 cactgaaggt tgtaactttt atacctcctg ctgtagagcc tgaagcacca cccacaaaca
    30001 tcagaaaaat agtcatcagc tttgagggtt ctgtcatttg agcgataggg agagaattat
    30061 aacctgctgt tctgggggta actgcatgaa accatgcatt tagtatctta tctgcccaag
    30121 gcaggccttt tagagtctcg gggttatttg attcaaaaac aaaaaaaagg acggaaccag
    30181 aaactatcaa gatggttgtt acaattagta ctattttagt gtgaagcatg aaagatgaaa
    30241 actttctatt ttgtacaaca tctttccaga caataaatcc aaggccgcca attattatta
    30301 ataacccgat agttattata ataacaggtt cccgattgag ttgtgtcagg cttatgaatt
    30361 catttcccac acttccgaat atatcaaacc ctgcattaca gaatgccgaa atagagtgga
    30421 atacactctt tgctatacca gattttaacc cgtatagcgg tataagcctt gtagaaagta
    30481 ctgcagctcc tataaattct attgcaaaag tagcataaag gattttttta aacaccctga
    30541 ctatgcctgt aagtgtaaat tcgttcagag attcctgtat ggccagcctt tcctttaacc
    30601 ctatacgtct tcccaaaaca agagaaaaca tggttgccaa tgacataatg cccaggcctc
    30661 cgatttgaat taatataatt ataacagttt gtccgaatgc agaccatgtt gtattggtat
    30721 ctacaacaac caatcctgta acacataccg cagaggttgc tgtaaataca gcatctagaa
    30781 aaccgacact ataaccaggc ttggtagata ctggaaggga cagtaggata gcacctgcta
    30841 atattaaaaa agcaaaacca aaaaccaaag ctttggtagg agaaaagtga gtcaattttt
    30901 cctctccatc cttcactgtg ttaaatttaa aaatgttcat aaataaatat tactacctcg
    30961 tttcccggat tgtaatgtat tattaatagt atatgctatt aataatattt aaaaagaaaa
    31021 tccgagtatc tatactttca atttaatttt acataccatt ttataatata tatttggaaa
    31081 aataaataca tttaataaaa tattttatgg tataattatt cttaaactgc attatatata
    31141 tgaaatgaaa cgaaaggctc aaatgaaata aaattgaatt taaagctttt attgtatttt
    31201 attttaagtt atgttacaat aactcaaaat atatgtctgc atacagagat gagaaatatt
    31261 tgactaaaga ttttttaaga aagcaataaa gaaaagaata ttaatagagg tagtaaataa
    31321 agattattaa gaactattaa ccaaaatctt tagaagaggt ggataataat ggataaaaaa
    31381 tttagagttg gggttattgg aggtactggt tttgtaggtc agcgctttgt gactcttctg
    31441 caaaaccatc catggtttga agttgcctgc attgccgcaa gtgaaaggtc tgcagggaaa
    31501 acatatgcag aagctgttga gggacgctgg aaacttaata tacctatgcc ggctgaactg
    31561 ggaaatatta ctgtaaaaaa tattaatcag gtagaagaag taaccggaga agttgatttt
    31621 gtattttgtg ctgttgatat gaaaaaagaa gagataaagg cctttgaaga gctatatgca
    31681 aaaacggaaa caccagtagt ttcaaataat tctgcaaaca gatggacttt tgatgtccca
    31741 atggttatac ctgaaataaa tgctgatcat ttgtctataa tagaaagtca aaaaaagaga
    31801 ctcggtacta aaagaggctt tatagcggta aagcctaatt gttctataca aagttatgtg
    31861 ccggcaatac accctttaat ggattatggg ccggagaaaa taatagtaag cacatatcag
    31921 gcaatttcag gtgcaggaaa agtattcaag gactggcctg aaataatgga taatgtaata
    31981 ccatatattg gcggagagga agacaaaagt gaaaatgaac ccttgaagat ttggggttcc
    32041 ataagcggaa acgaaattgt taaggctcaa aaacctgtaa tatccgctca gtgttatcgt
    32101 gttcccgtat ctgatggaca tatggcatca gtatctgttt ccttcagtaa aaagccttcg
    32161 gaatctgata ttttagaagc atggaaaagc tataagggga aaccacagat tcttcaactg
    32221 ccaagtgctc caaaacaatt tatcacatat tttgagggaa atgatagacc acagaccaaa
    32281 ctggacagag attgtgaaaa tggtatggga attactgtgg gtaggctgag aaatgataat
    32341 attctagatt ataaatttgc atgtctgtcc cataatacta taagaggtgc agcaggggga
    32401 gcggttttaa ttgcggaatt gcttgtagcg gaaggataca ttgcaaggaa gtgaaaaata
    32461 gctctaatct taatatatag gggttttctt atatttttgc ggtttagaag ggcaactggt
    32521 gtaaactgaa aatttgattt caatgttaat gactggtata atatttataa attatgaata
    32581 cagctgctta tattatatat aagcagctat accagcacat agtaaagatt atctcttgca
    32641 gaggtagggg cacctatgat acgcctggtt caggaatgta tgaaacttgc tctggtgtga
    32701 tcataattaa ttggaagtat atattgattt tattaatgtg aataaaatat gcacacaata
    32761 ttataggtat atatgaacca aataataacc aaggaggctg aatattatgc aggaaatagc
    32821 aacaataaat attgaaagca gggaaaatac cggtggtgga agtagtagga gattaagaaa
    32881 caatggatat ataccggcag tattatatgg gaaaggaatg caacctttgc ctgtaaaagt
    32941 aaaaaagtcc gaattaaggg ggacattact aaagtacgga aagaatgcgg tatttagtat
    33001 taaactaccc ggcaaagatg atacttcagc aattataaaa gaaatacaaa acgattttgc
    33061 tacaggtgaa attatgcatg cagattttca ccaaatatct ctgacagaag aaataaaggt
    33121 tgaagtacct gtaaaaatat tgggacgtga aattgttgaa gcagctcgaa tggtggtatt
    33181 gcaacagatg gatgaaatat ctgtaaaatg tttgccgcag aatgcgccgc aacatattga
    33241 aatcgatgta tcaaaaatgt ctccgggaga aagcattgct gcaggagatg taaagcttct
    33301 ggaaggagtt gctttggaga gccaacctga ccaaatagta gttacattga atgaagtaaa
    33361 agaaaacaaa gctgaggaag aaaatactga agctgaggaa actgaaacag ctgcagcaaa
    33421 cgaatgatct gtttggcgat aacattatca acttgctagt aaaaataata aagttaaaga
    33481 aattcttcaa aaaccgacaa agtctttatt gtataccttg tcggtttttc tatctttatt
    33541 tattgtagca aaatacataa aaaaaagtct atttatcaag aaaaaacagt caaatggtaa
    33601 atttattttg tacatattgt cctttatcag caatatatgt tacaattgac cttattaaaa
    33661 tataaatttg gaggaataaa aaaatatgcc agctgagtta gacttgaaag aaattatatt
    33721 cggaatttta ggtggacttg cattatttat atatggaatg aacctcatga gtgacagctt
    33781 gcaaaaagct gccggtgaac ggatgaggcg tatattggaa gttctaacag gaaacccttt
    33841 tataggagta ttggtaggag cactagttac agcggttgta caaagcagtg gtgctactac
    33901 tgttatggtt ataggatttg tcagcgcaag gcttatgact cttacgcagg caataggagt
    33961 aatattggga gctaatatag gcacaactat tacagctcaa atagtagctt ttaaactagg
    34021 cagctatgca tatgtaatag ctgcattagg ttttgttatt tactttttat ttaaaaaaag
    34081 gactataaaa tatataggac aatcaatatt tgcatttgga cttctgttta taggtctgaa
    34141 tttaatggga gatgtaatga agccccttgc acaaagctcg gaatttgcca gcataatgtt
    34201 aaggttaaat aaaaataggt ttcttggagt cctggttggc ctggtaagta cagtattggt
    34261 tcaaagcagc agtgctgtta ttgctgttgt tcagaatatt gctgctcacc ctgcagttga
    34321 tggggtacat gctatgatta atttggaaac agccatacct attctttttg gcagtaatat
    34381 aggatctact gttacagctc ttttagcttc aattggaggt aaaattaatg caaaacgtgc
    34441 agccgtagcc cattttatat ttaatatatt tggaactctg gtatttatat ggtttatacc
    34501 ggcttttgcg aaacttgtga aagttatttc acctaaaggt gatgaagttt tgatagtatc
    34561 aagacaaata gcaaatgctc atacttcctt taatatagta aacactatta tatggcttcc
    34621 ttttatatgg cttatgacaa aaatagtaac ttttattgta agaggtgagg atgacgaaat
    34681 agaaaaaaga gtactatatc tggataatag aatgcttaac aaccctgcta ttgcaatgaa
    34741 tcttgtcaca agagaactgg ccagaatggc gaagatggca caacagatga tgacaagtgc
    34801 acgccaggct tttgtaatgt caaatatgga agaatctaaa aaggtttttg aaattgaaga
    34861 aactattgac acgttacaga cagaaattgt aaaatatttg tcaaccatgc tgtcaagggc
    34921 atcacttaca gaaagacaat cgatcaggct tgcaggacta atgcatatta ccaatgatat
    34981 tgagcgtata ggtgaccact gtgaaaatgt tgctgaattt gccgaatgga aagttaatga
    35041 aaacattcca ttttctcagg aagcactgtc agaagttgca gatgcattta caatggttaa
    35101 caatatggta gatgatagca tatatgctct acatgatggt aatatcgaac tggcaaaaaa
    35161 agttttgtca gaggaaaatg aggtggacaa ccttgaggaa cgcttacgtg acaggcattt
    35221 gaagcggctt aattcaggac tttgtgatcc tcaggcagca gtaatcttta ttgaactaat
    35281 tcataatctt gaaagaattg ccgaccactg taataacgta gctgaggcag ttttaagtga
    35341 atacaatata aagcctgaaa ccatatagcg atataaatag taaacaatgt aaaccaatca
    35401 gtatggcttt aagtcatgct gattttcatc ataatattct taaatagata gtataatata
    35461 tataatatat atatattaac tcctaaactc attttattac ataatgtatt ttcagagagg
    35521 tataaaaaag gttctaattt acctggccat ataaaggagc gcataatata tgggagacaa
    35581 cagatacaaa aagataaatc aagtcctatg gattgttttg ctattaaata tatttgtagc
    35641 tttgattaaa attattacag ggtacattat aaaaagcggc agtttaaccg ctgatggatt
    35701 tcattcgatg acagatagta gttctaatgt aattggactt tttggcataa aacttgcctc
    35761 gaaacctgta gatgaagatc acccgtacgg acataaaaag tttgaaacca tggcaggttt
    35821 atttattgct gtttttttac ttatacttgc aggcaaggtt atcccggatg cagcaagaaa
    35881 ccttacacac cctgcagcac ctcaagttac cgttgagagt ataatagccc ttgtaattac
    35941 tttagcaatt aatatactgg tttcaaaata tgaattcaag caaggattga tattggagag
    36001 cgatattttg atttctgatt caaagcatac aaaaagtgac atttttatat caataggagt
    36061 cataatgact ttaataggta taaaactggg gctgcctccg attattgatc ctatcatttc
    36121 attagtggtt gcaggattta ttctttatgc atcttacgaa attattaatt ctaccggaaa
    36181 aattctcctt gataaagtag ctatcgatga agaaaagata agagacattg ttatggagtt
    36241 taaggaagtt aagggctgtc ataaaataag aagcagaggt agaaatgacg acatttatat
    36301 agatctgcat attcttattg accctggaat gagcgtagag gaatcccata ctctaactca
    36361 tggtattgag atgaaaatga gggaagaatt taataaaaat gtgcaggtta tagttcatat
    36421 tgaaccttat tatgagtttc attaatttgt tctgataatt tgggagcatt ttttatattt
    36481 tgcatcaaaa gaagaaaata tgccccgttg actttgccaa ttaattatat cctgaattct
    36541 gcacacaaaa aatggaaaaa cgcctttgca aagcactaat
//
LOCUS       DAOSDD010000009        36115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_607_length_36115_cov_5.971298, whole genome shotgun sequence.
ACCESSION   DAOSDD010000009 DAOSDD010000000
VERSION     DAOSDD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 36115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36115
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_607_length_36115_cov_5.971298"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..483)
                     /locus_tag="PK733_01555"
     CDS             complement(118..483)
                     /locus_tag="PK733_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOJ09264.1"
                     /translation="MYEVIFYQDSKGDSELLEFIEKLNEKAATSKNERIILKQIRFYI
                     NVLENLGTRAGEPFVKHIQDVLWELRPGENRILFFMWYENKIVLLHHFTKKTNKTPKR
                     EIEKALYEITEWRRRNSNG"
     gene            complement(706..1878)
                     /locus_tag="PK733_01560"
     CDS             complement(706..1878)
                     /locus_tag="PK733_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ09265.1"
                     /translation="MDIFEKYNLKRIINASGTMTALGASRVSKRISDAMAQILPEFID
                     MKDLQKVACEIISRLTGAEYGCLTASAAAAITIATAACMTGTDFYRISKLPDTQGLKN
                     EVVIQKGHSINFGSSIAQMISISGAKCIEIGEATSCGSYHLIGAINENTAAALFVVSH
                     HTVQSRLIKLKEFIRISKKKGIPVIVDAASEYDLRGFSEAGADIVIYSGHKFLGGPTS
                     GIIAGKKEMVRACYAQERGIGRGMKIGKESIIGIIEALEQWNERDTEAIRASEDKRIQ
                     FAYNFLNNIKRLKCEYSPDQTNNPITRLKVVVDPKISPLTAFELSVLLAEGEPSIRVR
                     SHHVDMGYFYLDPCNLIDGEMEIICDRIKTLMENPINKDYKTENLVDIAVNHLKLW"
     gene            complement(2009..3445)
                     /locus_tag="PK733_01565"
     CDS             complement(2009..3445)
                     /locus_tag="PK733_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09266.1"
                     /translation="MKFFISKEKITPDIPVLQSGFAARKHKSEGIYDDPYATVLIIKE
                     NKTVVIIALDLLYGDRSFAYGIKKSINEKYGLKQDEIIINYSHTHSVVRVTGEDEVSR
                     SVRPYSISMDKFLWDINGKDIDYAEDIRYFNIIKTKIMNMLDKGFQNLIECGAYICKS
                     KFKFGINRRYPSPEGILMKPNYDDNCIDTDLYLIKFVDINDNIIGLIYNYACHPVILG
                     SGNYLISADYPGVVRKYLEKCNTGMTPIFLQGCGADINPYIAADKDRFKSCNFDELEK
                     AGISLAKEIQRLIDNHSEKRTIENNIKLHENFEEKNEDCSSWKKVGSYIQTGYSEVKL
                     YTEIWDIPKWESITNDPKEPIYIRESAKKVLLDIKKGKIRNYLPYYISILILGGRTYI
                     IALEGEVVSNIGKSIKRILSNEDAIVLGYSNSSECYIPTRKILYEGGYESTSFISAKL
                     AGPFAHETEDIIVGRAIAMAKVEMSNNA"
     gene            complement(3566..4447)
                     /locus_tag="PK733_01570"
     CDS             complement(3566..4447)
                     /locus_tag="PK733_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09267.1"
                     /translation="MMKRSLGEKIFCVINIFVMIFVICVVIFPLLHIFSVSVSDRIAV
                     ASLQVGIWPKGFNFAAYQKILSNPVFKRSFINTVCLTIVGTFLSIIVITMIAYALSKE
                     FFFGKKLVTYFFVITMYFSGGLIPTYLVISNYLNLNNSYLAYILPSLVNVFYVIVVRS
                     QLETVPQDLIDSGYIDGANEFRVLFNIILPVITPTIAAVSMFIALGRWNMWFPVLLYS
                     TKENMWTLQYYLRNVVFGKFLENTKNAPQMVDADIIPPKNYQMASIILVALPIVSIYP
                     FVQKYFVKGILAGSVKG"
     gene            complement(4467..5411)
                     /locus_tag="PK733_01575"
     CDS             complement(4467..5411)
                     /locus_tag="PK733_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09268.1"
                     /translation="MVQNTSKLTTNINKSRGFKMSRDLPIYGLLLPGVILLIIFHYLP
                     LFGIVIAFQDFVPFKGVIASKWVGFRNFAYFLTDENFWLVMKNTLEINIMQIIFGFPV
                     PILFALFLNELWSNRFKKTVQTISYLPHFISWVVAASIVTSILSPSTGIVNQFLKNVF
                     GIQPIHFLAMPKYFRPIIIISSIWKEFGMNAVYYMAALSSIDLQLYEASRIDGAGKLR
                     QTWHITLPGIKNITIVLLVLQVGSLVTIGFERIFLLYNPLVYEVGDVISTYTYRIGIE
                     QARYSLTAAIGFTQSFVNFILVYLTNRLAKAFAGWALW"
     gene            complement(5558..7186)
                     /locus_tag="PK733_01580"
     CDS             complement(5558..7186)
                     /locus_tag="PK733_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09269.1"
                     /translation="MKRKIAALLVLISVVLMLFSACSKADISSNKSSEQDGASSSSST
                     TDTSGNAVKQPDKIRVFYVTAGIATPDNFNFQSNVILDEIAKIANVEITEVIVPPSSD
                     TITKYNLMMSSGNICDVVHLASKNTMINDGKNGAYVELSDIIKKSAIITERYLPYIDQ
                     LKADDGNIYFLISLPVDGDVNDGIAIRYDLLKDIGYSEIPVTMDEWLEAMRKLKSKYP
                     DSIPYASKDTLFWCEFVFKSYGCAGDGYSWQYYKDKIIHTFENPLYKEAIKTYRLMLE
                     EGLMDKEFVTTKEQDYQDKRLNNKVLVNPQNLASIGGWVNRYTENNITEAIFVPAQWA
                     KIDDPRIDPRSVYEGPLPLGTHCMGIASTSKQKDASIRFIEALLSDKGFELSVWGREG
                     IEFNIVDGKKVLNFQKSNETNWRFIYNMLFGYNTKEKVEVQLKTSIANTKFDDEGKRE
                     YERIYWEQFDKVYADANSVLMNPMKFIILEPDTTTRRTEARNEARTIAVKVILGELTF
                     EEFDVQASNFLKKYQFITDEYNQKLPAAKKRAGQ"
     gene            7636..9996
                     /locus_tag="PK733_01585"
     CDS             7636..9996
                     /locus_tag="PK733_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ09270.1"
                     /translation="MNYIKRIPIKRNFSTFLPSLLVSFISIIILCALIMGIPLSQTMT
                     SFITNQTISSNNKLLTQYKTSIDLVLVETVEDVSLKLMHDLNNNFYLNCYFNNPLAGN
                     LANIYDISKYLDNMSILNPLIFSLSIYYSNNNLLVSNDCIRHTLYKTLESQKYLNHYY
                     NIVQQAAEISLNNEEYVSLIFDYGKNLNFKSSNVQEKKIPETVIHAVRITNGYNNIRG
                     AVIVTVSGDIFKNFISKYAPEDLGSIYIFSKDGIIISHTDSTYIGHNISEFQYYNTLL
                     QVNNESGYFISNVEGIPVVISYQTSSYNNWTYVSVAPMDNISSLTNYILRTIILIALL
                     AMSVGIIISVAKAKKLAKPILSIADYCLKSPYSFSQEKNNNEYSLISGTLNNMENIMK
                     EKEEEFKQVLPMLKMNFLSALFSDNPPEMLEIKSRMKMLGISFQYKFFCAAVITFDKL
                     RNSEKVITYEYEKLLIQSQLENIFTTDSSLCLFYEKDNTMAVLFNFDFEDKVLYELGE
                     KFLKQTLKQAYDNMSITKCISFGKIDTNIRNMCTSFKLALNGLNYNYVFPEKKIFTFA
                     RIMFWEEKHSFSARLLLNNFDNSLKCLDKGKCKSDLQKLVETLRNGNYSYQQIYNTLI
                     NCISIVEDFICAQIGEAIDLDQGFRNTANILEFESWMKGIIDATIDDLANIECSSTNL
                     VKKAQAFIEQNIQNTQLSLEYIANELGVSAKHLSKIFKDKTGINYIDYLTNLKLSHCR
                     NLLINTNLKVEEISDIMGYSTSQYFISRFKMMFGCTPKKYREKYSR"
     gene            10439..11047
                     /locus_tag="PK733_01590"
     CDS             10439..11047
                     /locus_tag="PK733_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ09271.1"
                     /translation="MSIYGYIRISTNKQKTDRQWEALKSYEKENKIQFDAIFEDKQSG
                     KDFNRSQYKALKNSIKKGDVLVVKELDRFGRNYEEIKQELAYFQSMGIKVIILDLPVL
                     NVEDETLSSLLNNLIIELLSYIAQKEREKIQDRVKEGLQVARNKGVKLGRPERKLPDN
                     FEKYYKRWKADEITAVEFAKLIGVSRATIYNYINEYECKSKE"
     gene            12029..13009
                     /locus_tag="PK733_01595"
     CDS             12029..13009
                     /locus_tag="PK733_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09272.1"
                     /translation="MQGNCKNIELSDRDWNYIILRPGRLLCLKCLSGGGCLPFMEKEK
                     LKGKLDAIKDDPQIHIRLETSFDEIGARTTRFFQQSVAERKRDLDVLQKLGLSPGDVR
                     IARDLYVLIGERIKDVYDICGYGGSNSEKWPACPLAHEEYFSKGTEGMNKLKDKDKMS
                     SWKKISCDEIEQANRIVIRAHHLLCLFCYIARDDFGGEYVPLAEDNLYEVWIKMRENL
                     DIPVTVIEGPGDCMICPPCHGFDNERKICFVACHLRDRKKDTDTLQKLDLLPGETIPA
                     NEVIKRVYEKIPDNSWICAYEYESAPQWRSCGGHERYKIGLERGFFVSKE"
     gene            complement(13749..14657)
                     /locus_tag="PK733_01600"
     CDS             complement(13749..14657)
                     /locus_tag="PK733_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09273.1"
                     /translation="MSHNEVKIITFENINLDEIAKVGRTVIQESEKSIEYHTHNYCFE
                     IIYHYTGEQKYKINGEEYKISGGQILIVRPGELHGSGKYISERKVYYNIVFHISPKDL
                     SFLGMTECDTQKLKMILQGKQRIFSIRNGLKTLFDDIINFNMQDYGLHSAFYKASILI
                     LFNRIYASCIAEHNKIPNYINELATYIDKNPNEIIDFEKFAYSKSISPRKLKYLFTLH
                     KGLSPSDYVMRARINRAEELLSNTNLSITDIAFEMGFSSSQHFATTYKKYKHRTPSQY
                     RNACLSKNSEIQSQNLDWHILNSNMR"
     gene            15065..15871
                     /locus_tag="PK733_01605"
     CDS             15065..15871
                     /locus_tag="PK733_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09274.1"
                     /translation="MEKMIPTWYYQQFDADYSSEFPGEGYGGWKNTSLPINPDKTAIV
                     LMHAWDTGTIEQYPGWFRAVEYIPRATHICDNLLPHFLKKVRSSNIRLIHIASSNQIV
                     NDYPGYAKTLEICGEDTFGYERVEEDETIKKLNYFRGENVFVGSHNKEDVARGMKHLD
                     FYPTARPLDNEEIVCTSEQLLNLCKKYGINHLIYTGFAINMCLTVSPGGFVDMSRYGI
                     MCSAIKELVTAVENKESCRNEANKNYGLWYIAILSGFVYEMGDIEKYLLD"
     gene            15960..17738
                     /locus_tag="PK733_01610"
     CDS             15960..17738
                     /locus_tag="PK733_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09275.1"
                     /translation="MKNTKLIRILVLTLVSMMLLSACSSKTGTNSSSSSTNSKENTSA
                     TSTPKVGDSQKFDPNNVTRKNPFEKFNPVIELTTVRFEMPFLIFENGDNIDVNDWTRA
                     YENELGIKVKNLWVVDSSQLTQKTDLMIASGDLADVFQVSDIQFQQLIENNLIEDISQ
                     AYLEYISDDAKMILTEGGPYALDAAIRNGKLMGIPFTGNQLESISLTWIRTDWLKALN
                     LSVPTTQKELEDVLIAFATKDPDGNSKADTHSLVLTKDAFKTSSHQDALFYSYNAYPN
                     TWYDDGSGNLIWGGIQPQIKDGLAAFKRLYDAGAINREFTTIDLAKAKEAVVADKIGM
                     IYEGYYGSGGILQPHVEATKKTDIWQAYPIPSSDGKAVKLPHDTATYGFHVVRKGFKY
                     PEAAIKMLNMWIQEFYINTTDNDRYLQLIQNETGTNAKWNLSLCQAYRPLNNINNFRN
                     LEKWKETGNKEYYDKVTYLGKSFYNGGIRFKENGEISQWFWWVMHCPEYENPAMRITS
                     KNIQNKNLRPNLFYGVPTPTMTEKWKLLCDKQAQAYQSIIAGGSLDDFDKFVNEWKSL
                     GGDQIVKEVNVWYQDHKNAADNVYVK"
     gene            17902..18759
                     /locus_tag="PK733_01615"
     CDS             17902..18759
                     /locus_tag="PK733_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09276.1"
                     /translation="MMVPAIVLVIIFSYIPMAGIIIAFQKFVPAKGLFGPQQWVGLDN
                     FKYILSMPDTGRVFRNTIYIAVMKMIAGQLVPIAIAILLNEIRNRSYQRWIQTFIYLP
                     NFLSWVILAGIFINILSVKGMVNQLIAFLGGKPISFIGNKIIFPYTLVATDVWKNFGF
                     NSIVYLAAITGIDPSLYEAATVDGANRMRRMFHITLFGMLPIIVLLATINLGSILNAG
                     FEQVFNLYSPTVYETGDIIDTMVYRLGFDNAQYSIATAMGLLKSVVSTILISVSYYLA
                     YRFANYRVF"
     gene            18773..19702
                     /locus_tag="PK733_01620"
     CDS             18773..19702
                     /locus_tag="PK733_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09277.1"
                     /translation="MSKASKKGKRTKHNIIPTGSLLFDVLNHIFLGIVALSCILPLIH
                     VLAISFSVSWAAQGGIVYFWPVGFNLESYKFILKNGKFITALIVSLKRVTLGVAINLF
                     LIILTAYPLSKEEHDFKGRTLYAWFLFITILFSGGLIPSYMNIRSLGLLDNILALILP
                     GAVPVFSVVLLLNFFRQLPKGVEESAFVDGASFWTTLWKICVPMSLPSIATIALFSAV
                     AHWNSWFDGIIYMNRTDKYPLQSYLQTIIMMASSSNEFIRATPEMAELLKKINDSTLK
                     AAQIFLGALPIILVYPFLQRFFMKGIVIGSIKG"
     gene            complement(20367..21749)
                     /locus_tag="PK733_01625"
     CDS             complement(20367..21749)
                     /locus_tag="PK733_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09278.1"
                     /translation="MKKIMFFDDFIVNRTDNTRRVFKEPVWDFSNAFNEPGSLRGILG
                     ESVIPAPDGGYFLYYLTVPKDKKFIDENLMVCTAYSEDGISFLNYNHLHPRYPDMPYA
                     LGHTYEYIGIYGYFDKYEKTPDYRYKAVCATYGESPKGLIEEAPVMLVSSDALHWKKP
                     NDVKVIPSYVDCYTSLLYNPVTGNYQTTSRRRWGERRICLVESRDLKSWSMPRAIIHP
                     LPSDEPTTHLYGMPHFYYEPGDIFIGMLWKQIMPYNRIMDGPVLTEYAYSYDGLTWNR
                     THAPVMKQRNRGELGGGSSYVFSMMELGDELIFHANACKTEHGGPSNFVSTDGEAAKQ
                     VVTGRMKKDRFVCIDSGKGAAEMTTQNLKIRSSELTLNLSAPFGRVKAQVLNNNGVVE
                     GFAYDDCDPIQGDHLAIQLRWRGKNFEEICKRDEWIKLQIAFEQAEIYSITGDFVFTI
                     NTRAPIYDRL"
     gene            complement(21775..23982)
                     /locus_tag="PK733_01630"
     CDS             complement(21775..23982)
                     /locus_tag="PK733_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09279.1"
                     /translation="MKNGLKSRISKRFFYKILAIMLLLSLLPYIVFNLYFSSKIKYEL
                     INYIRDISYIELVSSTNNISSSITQMQDILYYIASTPGIFENLNNGLENIGILTKKRY
                     LDTLGQLASITGNKVRVCLYDPSNGIIMESNYGITDITNPRYSWLPLEVQKANENKQI
                     YISPARLIQYSEQIEYFSVIIHPVGANNKSDVVSFIFLNTGVLMKELLNNTSNAGDAY
                     TCYIINAEGSIIYHSNNDLIATRLPPNINKSLIEDGKYQKESVVMSAFPISGTTLYLV
                     ADINTEKLYAWVDKLIHLIFYITIVIMAILSIAAIKLSNVLYGPVHKVMRDVSIKLGY
                     KSFQKDEFEFISVAFNNIENKYQQANSDLKTIHYILHRATLLNHLKMGQEQEVIEVVS
                     DQITSMSKQQTYAVLLLKNDEIFESDQYKEILNMSLPNSEYFNNNDKLICVFPYETSM
                     NDLKKLLQEDFYDKLAYKGCTVSICQNTRMLKELHRAYSEAMFTCILANFLNIDGIVG
                     YNEILNMALGYIPLESEQVDELKYCLVKFDEKKFSKIINDYLPSGLPAIEFNKRICAM
                     IAYMINHFKEDASVYINEFIDYIESNDKLDSSKMRSFFEKCFRSSSNRNSEEKSGIHI
                     YLKKMNQFIEKNFDKLISLDDVSDYLGISKQYFCYIIKHEYNTTFINYLNKYRVEKAK
                     LLLNNNETRINSISGQVGFSSDSYFIKVFKSITGLTPGEYQNYVLSNIDTNNN"
     gene            complement(24142..25029)
                     /locus_tag="PK733_01635"
     CDS             complement(24142..25029)
                     /locus_tag="PK733_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09280.1"
                     /translation="MKYKNSKSDIVLIGIAYTTVIILTFSIIIPFLLIFLQSITPRSA
                     MTGEWYSIIPKQVTFDAYGYLLVSSKLIIKAFKNSFFLVVIGGSLSLIATTMAAYTLS
                     KQYLPYRKILTYFVYIPMIIGGGLIPTFLVVKFIGLYGSLWACVIPGMVGSWNVFLMR
                     NFFREIPESLEEAALLDGANDVQILVKVMLPVSLPAIATIGLFYAVGQWNSWFTPSIY
                     LNSREQWPVQMVVRQMLAQFDWTSVESIQDMEELLINIPTDNVKKASVFIATLPVLVF
                     YPFAQKYFVKGLVVGSIKG"
     gene            complement(25098..26045)
                     /locus_tag="PK733_01640"
     CDS             complement(25098..26045)
                     /locus_tag="PK733_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09281.1"
                     /translation="MNTLAQSSRKPKAFIHNIIRYKFLYLLILPTIVLFLIFNYFPMY
                     GVVIAFQDYKPHLGVEGMFSKATWVGLKQFKNFISSFYFKRLMINTVRISLLKILFGF
                     PAPIILALFLNEIRNLKFKKIVQTVSYLPHFLSWVILSGIITSLLSPANGTINQFIIA
                     LGGQPVDFLTNNDYFIWVLVFSSVWQGVGWSSIIYLATLSGIDPQLYESSVIDGATRF
                     QQILYISLPSLYNLMSILLIMSMGGILNAGFEQVLMLYSPLVFETGDIIDTFVYRSGI
                     QELKYSYSSAVGLFKTAIGFTLLIITNFFAGKMGRETLF"
     gene            complement(26193..27761)
                     /locus_tag="PK733_01645"
     CDS             complement(26193..27761)
                     /locus_tag="PK733_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09282.1"
                     /translation="MRVKSKVIILVVMISVMFSLFSACQVQKTEQQKTTTSEVHSKKE
                     TPVVLVWPNVDEGYSAQLKTDASKQVHNKILEVLNVDIRPVFYPIDQYQNRVNMLIAS
                     GDQVDIIPRINRQKSIEYFNDGVIIDLTELIKNKAPDYLTACEKYEFIAKAREESKYQ
                     GKDLVMPWPTLVPRGQTLQIRTDWLSKLNIDIPTTLEGFEAYLEAVKAKDPNNTGKDD
                     TYGLSAGVWGANIVTIMSMFYCPTGWERWLDESGKLQIAELHPNYKLLLEDLARWYKA
                     GYLPPEVFTSADDQRNDWVTSNKVGALATWYSAPIAGIVTLRKSVPEANYLPICIKGK
                     PEAANAYRNENATPNSPVITTSSKNPEAAMKFLNYLYTNEGQILCTWGIEDISYKLID
                     GQPDSILGEDGNKLYYGVYLPQLSIPQLQFGLFAGPAPNLKLYRELADVLKTLPGYDP
                     PDKLVAYDTKAFKSEAKLADMDTFLQEQRLKVLVGEIPVSEWDNIMSRWKTMGGEQYL
                     EDINLQYKAWLGSK"
     gene            complement(28271..28753)
                     /locus_tag="PK733_01650"
     CDS             complement(28271..28753)
                     /locus_tag="PK733_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ09283.1"
                     /translation="MALRISDLLELTWRDVLKENKDFKVIEIKEGKYQKQRKIKLNRS
                     AQKAQGELLESLDTYDYIFQSREGDNKSITRQQALNILKDAAAAIGVEERFGTHALRK
                     AWGHHDWKKGFNPALIMETLNHNNLNVTKRCLGNTARILFILMDLMEQIKAVVPKSNY
                     "
     gene            complement(29653..29790)
                     /locus_tag="PK733_01655"
     CDS             complement(29653..29790)
                     /locus_tag="PK733_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09284.1"
                     /translation="MKMTIPYNITGKQRKQLADDISVALHTIPKYMGYPTCSYQIGGC
                     T"
     gene            complement(29768..30016)
                     /locus_tag="PK733_01660"
                     /pseudo
     CDS             complement(29768..30016)
                     /locus_tag="PK733_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
     gene            30217..30432
                     /locus_tag="PK733_01665"
     CDS             30217..30432
                     /locus_tag="PK733_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ09285.1"
                     /translation="MATSYNKLWKLLIDKKMSKADLRKASGVSPNTMTKLRRDEPVML
                     SVLDKICKVLDVNYGDIVDYIPDIENE"
     gene            30457..32322
                     /locus_tag="PK733_01670"
     CDS             30457..32322
                     /locus_tag="PK733_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ09286.1"
                     /translation="MNRIDIINRIGDKYHVGNTENGEFSYVQALKSVGKDINDYQKAT
                     TGTKHQFLDIRFENDRLVVLVECKNKFSKWPKDRIQAQLQDYVRFEKAYSDKKIVAIL
                     AETDGDDIWVWYGQSVIIDDEHRNDEETTLKTFEEYENLCFGKVNDKIKVVDSIKTLN
                     EMLYSDGINEKLRSQFVGTCLLALKNGLTYKDVKETINPSTGKKLSPEQVVLRGIKDI
                     LEGLLTKSGSINKASKLAILNNKVLDDQDIASLTYAELKAILSYIDENVVPYINDKNT
                     AGQDLLNLFFTTFNKYVGKSDKNQAFTPDHICDFMSKAVGVNKNSRVLDPCCGSGAFL
                     VRAMTDAMDDCDTEKERENVKRNQIFGIEYEEGAFGLSSTNMLIHGDGNSNVIQDSMF
                     NRAKWIEDNNINIVLMNPPYNATKKNCDPEYTKKWDSKKKEDPSKGLHFVEWVARHCP
                     STCKMAVLLPMQAAIGNTGEVKTFKKKMLDNYTLDAVFSLPTEIFYPGAAAVACCMIF
                     DLSQKHEKANKETFFGYFKDDKFIKRKGLGRIEKTDSEGNTLWNRTEEQWLDLYKNKK
                     VVTGLSVMHKVSWKDEWLAEAYIETDYSSLTKSDFQQTINDYLSYLIKEGYVYES"
     gene            32312..33391
                     /locus_tag="PK733_01675"
     CDS             32312..33391
                     /locus_tag="PK733_01675"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ09287.1"
                     /translation="MNLDFKNWKTFDVNDIFYLFNGKGITKEEIEENPGDFFAVQSGA
                     ENNGCIGRIDKDYCINKGYTYTDEPCLTVARTGTAGFVSYQHYGCVVGDSAKILLLKN
                     KEKRSTPIYLFLRTILIELQYKYTYGRKVTENKYLSEKILLPAIDKETPDWMFMENYI
                     CSLDYKMPKTTVRLSKDMDLKTKEWKEFYLHKLFNAGMGNGIDAILTSSDNPMYNYVS
                     RDSNGNGVVGFVDEIDGQEPFPKGALSLALGGSFLGSCFIQKENFYTAQNVAVLQEKF
                     PMSNHVKLFIATLIRNESKIKYQAFGRELNSHFRKDFTIKLPIKHDKNGIVYDSSLEF
                     SDLGYIPNWEWMEQYIKSLPYSDKF"
     gene            complement(33820..35031)
                     /locus_tag="PK733_01680"
     CDS             complement(33820..35031)
                     /locus_tag="PK733_01680"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOJ09288.1"
                     /translation="MITSEEIKRRLWDGANELRGSMDASRYKDYMLGLMFYKFLSDKT
                     LDAFKVSAGLGKVTEEELVKEYKKAREAYGETLDKTIQGVLGYYVLPEYLYQTWIMDI
                     NTGDFEVHKVTDSLHNFERSVAIESGNDDFKGLFSSSTIDLTDTALGSNLNERSKNIQ
                     ALIMLFADLNMVALQKGDILGDAYEYLIGQFAMESGKKAGEFYTPHQVSEVMAQIVAK
                     SSKIESIYDPTVGSGSLLLTVGKHLDEDGQKNLNYYGQEKNTATYNLTRMNLLLHGVR
                     PEKMTIRNGDTLSYDWPEDPERPNEGVQFDAVVMNPPYSVKNWNRSGLKVSDPRFEIA
                     GVLPPDSKGDFAFLLHGLFHLRQSGTMAIVLPHGVLFRGGAEGEIRKRLIEKKDLDTA
                     ESQILRDFSAL"
     gene            complement(35054..35278)
                     /locus_tag="PK733_01685"
     CDS             complement(35054..35278)
                     /locus_tag="PK733_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09289.1"
                     /translation="MSEELIKIVTDKVQFVSKGQLNEFAFLKKILALKMSIIQRLKMN
                     GLIVGKVRRLSKLMNRLQLDKKKWDSADKL"
     gene            complement(35308..>36115)
                     /locus_tag="PK733_01690"
     CDS             complement(35308..>36115)
                     /locus_tag="PK733_01690"
                     /inference="COORDINATES: protein motif:HMM:NF037213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOJ09290.1"
                     /translation="KRQALDSDIIKNATNVIDGKMIVSNSDGGQFVIAEEKEPEMIAK
                     RMLLCVQYYLNKYSMDNIQVLCPQKKGMIGTAAMNERIQELVNPPSPNKRELRIGYFT
                     FRTGDKVFHNKNNYEINKYNYINNEYEVIVDDNSEPVQGVFNGETGIIADIIDLFDEK
                     EEDTFTKVVVKYDDCYVLYGAEEWEQLEPAYSLTVHKYQGSQNKVIIIPISFHNYKLL
                     SRNLLYTAITRAEDIVVIIGDPKAMYYSIKNVMLVERNTMLKGLLQGNTI"
BASE COUNT        11789 a         6227 c         5889 g        12210 t
ORIGIN      
        1 agttcttgcc gtctttcaat aatcttagtt attatatcag acataatgtc aatttccctt
       61 ttctcttctt cacttagaga tgtcattgaa gctttaacat cctgccactt aacaaactta
      121 tccattacta ttcctccttc tccactctgt gatctcatat aacgcctttt ctatctctct
      181 ttttggagtt ttatttgttt tcttcgtgaa atggtgtaac aatactattt tattttcata
      241 ccacataaaa aataatattc tattttcacc cggtctaagt tcccatagta catcttgtat
      301 atgttttaca aaaggttctc ctgctctagt acctaaattt tcaagaacgt ttatataaaa
      361 ccgtatttgc tttaatatta tacgttcatt cttacttgta gctgcttttt cattaagttt
      421 ttcaataaat tctaaaagct cactgtcccc tttactatcc tgataaaaaa ttacttcata
      481 cacctattaa tttctccttc atttgatgat ttatttatca tcaatgttat attaatgata
      541 acatatttat catatttctt caagagtcca aatgtgaact atacactacc tacactctca
      601 ctttgcgagc agggaaatta agaggcgggc gaattctttc cgaaaaggtt aaaattatct
      661 atgggtgttt tatccttaaa atatatagtt ttaatatact aactatcacc acagttttaa
      721 atgatttaca gctatatcta caagattttc tgttttgtaa tctttattaa ttgggttttc
      781 cattaacgtt ttaattctgt cacaaattat ttccatttca ccatctatta aattgcaagg
      841 atcaagataa aaatatccca tatcaacatg atgagaccta acccttatag atggttctcc
      901 ctcagctaga agaacactca gttcaaatgc agttaatgga cttattttcg ggtcaacaac
      961 cacctttaat cttgtaattg gattattagt ttgatcaggg gaatattcac atttaagtcg
     1021 ttttatatta tttaaaaaat tataagcaaa ttgtattctc ttatcctctg atgcccttat
     1081 agcttctgta tcacgctcat tccactgttc caatgcctct attatgccta tgatactttc
     1141 ttttccaatc ttcatgcctc ttccaatacc tctttcttga gcatagcaag ctcttaccat
     1201 ctcctttttg cccgctatta tacctgaggt aggtccgcct aaaaatttat gcccgctgta
     1261 aatcacaata tctgctcctg cctcggaaaa cccgcgaagg tcatattcgg atgcagcatc
     1321 aactattact ggtatgcctt ttttctttga aattctaatg aactccttca atttaattaa
     1381 cctgctctga acagtatgat gagatacaac aaataatgct gctgctgtat tttcattaat
     1441 tgctccgata agatggtaac tgccacatga tgttgcctct cctatttcaa tacactttgc
     1501 tcctgatata ctaatcatct gagctatact tgacccaaaa ttaattgagt gacccttctg
     1561 tataactact tcattcttta aaccttgtgt atcaggtaat ttactgattc tatagaagtc
     1621 cgttcctgtc atgcaagctg ccgttgcaat agtaatagct gcagctgcac tagcagtaag
     1681 acatccgtat tcagctcctg tcaaacggga aatgatttca catgcaacct tttgcagatc
     1741 tttcatatca ataaattctg gtaaaatttg cgccatcgca tcagatattc ttttactcac
     1801 tctagatgca cccagagcag tcattgtacc tgaggcattt ataattcttt taagattata
     1861 tttctcaaat atatccattt acttacacct cgaaaactta ttaaaagacg attatcaaat
     1921 agctaccata tcattgccta aggacatttt ctacactttt tattttttac atatttacct
     1981 ttcactttat ttcttactta tttttcaatt aggcattatt actcatttca acttttgcca
     2041 tagctattgc tcttcccact ataatatctt ctgtctcatg agcaaatggt cctgcaagtt
     2101 ttgctgaaat aaatgatgtg ctttcataac caccctcata taaaattttt cttgttggta
     2161 tgtagcattc actactattt gaatatccaa gcacaatagc atcttcatta cttagtattc
     2221 tttttatgct ttttccaata ttactgacaa cttcaccctc aagcgcaata atatatgttc
     2281 ttccgccaag tattagtatt gaaatataat aaggaaggta gtttctgatt ttaccttttt
     2341 ttatatccaa aagaactttt ttggctgatt ccctaatata tatcggttct ttagggtcgt
     2401 ttgtaataga ttcccatttt gggatatccc atatttccgt atataactta acctcactat
     2461 atcctgtttg aatataactg ccaacttttt tccagctgga gcaatcttcg tttttttctt
     2521 cgaaattttc gtgaagtttt atattatttt ctatagttcg tttttcacta tgattatcaa
     2581 ttaacctttg aatttccttt gccagggata tgcccgcctt ttccaactca tcaaaattac
     2641 aacttttaaa tcggtcttta tccgctgcta tataaggatt tatatcagca ccgcaacctt
     2701 gaagaaatat cggagtcatt ccagtattgc acttttcaag gtatttacga acaaccccgg
     2761 gataatctgc cgatatcaag taattgccag atccaagaat aaccggatgg caagcataat
     2821 tgtatataag ccctattatg ttatcgttta tatctacgaa ctttataagg tataaatcag
     2881 tatctataca attatcatca tagtttggct tcattaatat accttctgga gacggatacc
     2941 ttctatttat accaaattta aacttgcttt tacatatgta ggcaccacat tcaataagat
     3001 tttgaaaccc tttatctagc atattcataa tcttggtctt aattatattg aaatatctta
     3061 tatcttcagc ataatctata tctttcccat ttatatccca taaaaactta tccatgctta
     3121 tactatatgg tcgcaccgat ctgctcactt catcttctcc tgttactctc accactgaat
     3181 gagtatggga atagtttatt ataatctcgt cctgttttag tccatacttt tcatttatag
     3241 acttttttat accataagca aagcttctat ctccatagag caaatctaat gctattataa
     3301 ccactgtttt gttttcttta ataattaaaa ctgtggcata tggatcatca tatatacctt
     3361 cactcttatg tttccttgct gcaaatccac tttgaagcac aggaatatct ggtgttattt
     3421 tttcttttga tatgaaaaat ttcattattc tatacctcca atattaggtt aattgtcact
     3481 attaccttca gcactaactt gattgtctca atttaacata tcattaatac ttattggcct
     3541 tttccattta tttcataagt tcatattatc ctttaacact acctgccaga atacctttaa
     3601 caaagtattt ctgaacaaaa gggtatattg agactatagg gagagcaaca aggataattg
     3661 atgccatctg ataattttta ggcggaatta tatcagcatc taccatttgg ggtgcattct
     3721 tagtattctc taaaaattta ccaaaaacaa catttcttag ataatattgc agtgtccaca
     3781 tgttttcttt tgtgctgtat aataggacgg gaaaccacat attccatcgt ccaagggcaa
     3841 tgaacatgct aactgcagcg attgttggtg ttattacagg caatattata ttaaacaata
     3901 ctctaaattc attagctcca tctatatatc ctgaatcaat gagatcttga ggtactgttt
     3961 ccaattggct tctgactact ataacataaa atacgtttac aagtgatggt aatatgtacg
     4021 caagatatga attattaaga ttcaaataat ttgatattac cagatatgtt ggaataagcc
     4081 ctcctgaaaa atacattgta attacaaaaa aataagttac aagttttttc ccaaagaaaa
     4141 actccttgga taatgcatat gctatcatag tgataacaat aatcgataaa aatgttccaa
     4201 caatagttaa acatactgta ttaataaatg aacgcttaaa aaccggattg gataatattt
     4261 tctggtatgc agcaaaattg aaacccttcg gccatatccc gacctgtaaa gatgcaactg
     4321 caatcctatc acttactgat acactgaata tatgcaacaa tggaaaaatt acaacacata
     4381 tgacaaaaat cataacaaat atattaatta cacaaaatat cttttcaccc aagcttcttt
     4441 tcatcatttt catgtctctc cttcactcac cataacgccc acccggcaaa agcttttgcc
     4501 aaacggtttg ttaagtatac aagtataaag tttacaaagg actgagtaaa tccaatagct
     4561 gcagtaagag aataacgtgc ttgttcaata cctatccgat atgtgtacgt acttattaca
     4621 tcaccaactt cgtaaacaag aggattatat aacaaaaata tcctttcaaa tcctattgtt
     4681 actaaagaac ctacttgtag taccaatagc actatagtta tattttttat tcctggtaaa
     4741 gttatgtgcc atgtttgtct tagttttcca gccccatcta tcctggatgc ttcatacaac
     4801 tgtagatcaa tagaagaaag agctgccatg tagtatactg cattcatccc aaactctttc
     4861 catatggatg aaataataat tataggtcta aaatatttgg gcatggcaag aaagtgaatt
     4921 ggctgtatcc caaaaacatt ttttaaaaac tgattaacaa tacctgtact cggtgaaagt
     4981 attgaagtaa caatgcttgc tgccacaacc caggatatga agtgaggcaa ataacttatt
     5041 gtctgaactg tttttttaaa tctattagac cagagttcgt ttaaaaatag agcaaataaa
     5101 ataggtactg ggaaaccaaa aataatctgc attatattta tttcaagtgt atttttcata
     5161 acaagccaaa agttttcatc cgtaagaaaa tatgcgaaat ttctaaatcc tacccattta
     5221 cttgctataa cccctttaaa cggtacaaaa tcctgaaaag ctattacaat tccaaagagg
     5281 ggtaaatagt gaaatataat gagtaaaatt actcctggaa gtaaaagacc gtaaattggc
     5341 aaatctctac tcatcttaaa acctcggctt ttatttatat ttgtcgtcaa tttgcttgtg
     5401 ttttgtacca atattgtcac ctcctgccct tctgctatta gtcaaaccat taaaaagcta
     5461 taaacagttc ttatataacc gaaggcgtta cttctaaggc cccggttata taagaacaaa
     5521 ttatatttta gttacattat tataattgcg taatttatta ttgccctgct cgtttctttg
     5581 ctgcaggtag tttttgatta tattcatcag taataaattg atatttttta agaaagtttg
     5641 atgcctgtac atcaaattct tcaaaggtta gctcacctaa gattaccttt actgcgattg
     5701 ttcttgcttc attacgagct tcagttcttc ttgttgtagt atcaggttca agtattataa
     5761 atttcatggg attcattagc actgaattgg catctgcata taccttatca aactgctccc
     5821 agtaaattct ttcatactct cttttacctt catcatcaaa tttagtattt gcaatacttg
     5881 tttttagttg tacttcaact ttttcctttg tattatagcc aaataacata ttgtaaatga
     5941 aacgccaatt tgtttcatta cttttttgaa agttaagaac tttcttgccg tctacgatat
     6001 taaactcaat accttcacgt ccccataccg agagttcgaa acctttatcc gacaataatg
     6061 cttctataaa ccttattgaa gcatcttttt gcttgcttgt cgaagctatt cccatgcagt
     6121 gagttcccaa aggaagtgga ccttcatata cacttctagg atcaatcctt gggtcatcta
     6181 ttttagccca ctgagctgga acaaatattg cttctgttat gttgttttct gtataacggt
     6241 ttacccaacc acctattgaa gcaaggtttt gggggtttac taatacttta ttgttaagtc
     6301 ttttatcctg ataatcctgt tctttagtcg ttacaaattc tttatccatc aatccttctt
     6361 ctagcattag tctatacgtt tttattgcct ctttgtataa aggattttca aaggtgtgaa
     6421 tgatcttatc tttatagtat tgccaactat atccatcccc agcacaaccg tatgacttaa
     6481 atacaaactc gcaccaaaaa agtgtatcct tgctggcata tggtatggaa tctgggtatt
     6541 ttgatttaag ttttctcatc gcttccagcc attcatccat agttacagga atttctgaat
     6601 atcctatatc tttaaggaga tcataccgta tggcgatgcc atcatttaca tcaccgtcaa
     6661 caggtaatga aatcaggaaa taaatattac catcatccgc ttttaattga tcaatatatg
     6721 gcaaatagcg ttctgttata atggcagatt tttttattat atcagacaat tctacatatg
     6781 caccattctt tccatcatta atcattgtat tttttgatgc aagatgtacg acatcacaaa
     6841 tattgcctga tgacatcatt aagttatact tagtaatagt atctgacgat ggtggaacaa
     6901 taacctcagt tatttctaca ttagctattt tagctatctc atcaagaata acattactct
     6961 gaaagttaaa attatctgga gtagctatac cggcagtaac ataaaatact cttattttat
     7021 ctggttgttt tactgcattt cctgatgtat ccgtagtaga tgacgaacta gaagctccat
     7081 cttgttctga tgatttatta cttgatatgt ctgcttttga acaggctgag aacaacatta
     7141 aaactacaga tattagcact agaagtgctg caatttttct tttcatgata gataatacct
     7201 cctattattc tctaatttgt caagttatta agcaacacat ttaattaagt tattcaagca
     7261 caagcattaa aaagttaagt tacagtagtt gaatagagtt taagtagcaa ttttacagta
     7321 ataaaaactt gatatgagat atgcgcacat cttcttatgc aactaattat attctgatat
     7381 agaacttaaa acaatgttaa tttattctga agtaaatatt gttatttatt agtgtaatac
     7441 ctatagtttc aagctataca ttgcatattt tatggataac aatttttccg catacaatga
     7501 ttttagataa tttcatacat aatccatata cattatgata taattttatt gattcttatc
     7561 atgacatcag tataaaattt ttgatattat cttattctat atcacgtcat aacattaaat
     7621 aagggggttg tagtattgaa ctatataaaa agaataccaa ttaaaagaaa tttttcaact
     7681 tttttgcctt cgcttcttgt ttcttttata tcaataataa tactttgcgc tcttattatg
     7741 ggaattccat tatctcagac aatgacaagt tttataacta accaaacaat atcttcaaat
     7801 aataaattgt tgactcaata taaaaccagt atagatttag tcctagttga aacagttgag
     7861 gatgtttcat taaaattaat gcatgatctt aataataact tttatcttaa ctgctatttt
     7921 aataaccctt tagcaggaaa tctcgccaat atctatgata taagtaagta tttggataac
     7981 atgagtattt taaacccttt aattttttct ttatcaatat attattctaa taataatctt
     8041 ttggtatcta atgattgtat ccgtcacacc ctatataaaa cgttagaatc tcaaaaatac
     8101 cttaaccatt attataatat tgttcagcaa gcagctgaaa ttagtctaaa taatgaagaa
     8161 tatgtatcac ttatttttga ttatgggaaa aacttaaact tcaagtcttc taatgttcaa
     8221 gaaaaaaaga ttccagaaac agtaatacat gctgtcagaa taactaatgg atataataat
     8281 attagaggag ctgttattgt tacggtaagt ggagatatat ttaaaaactt tataagtaaa
     8341 tatgctcctg aagacttagg tagtatttat atatttagca aagatggtat tattatttca
     8401 catacagata gtacatacat aggtcataat atatcagaat ttcaatatta taacacctta
     8461 ttacaagtaa ataatgaatc tgggtatttt atatcaaatg ttgaaggaat ccctgtagta
     8521 atatcatatc aaacttcatc atataataac tggacatatg tctcagtagc gcctatggat
     8581 aatatttctt ctttaacaaa ttatatacta aggacaataa tattaatagc attacttgca
     8641 atgtcagtag gaataataat atctgttgct aaagccaaaa aacttgctaa acctatatta
     8701 tctattgccg attactgctt aaaaagtcca tattccttta gtcaagaaaa gaataataat
     8761 gagtactctc taataagcgg aaccttaaat aatatggaga atataatgaa ggaaaaggaa
     8821 gaggaattca aacaagtatt acctatgtta aaaatgaatt tcctcagtgc cttgttctct
     8881 gacaacccac ctgagatgct agaaataaaa tcaagaatga aaatgcttgg tataagtttc
     8941 caatataagt tcttttgtgc tgctgtgata acatttgata agctcaggaa ttctgaaaag
     9001 gtaattacat acgagtatga aaaactcctt atacaatcac aacttgaaaa catattcact
     9061 actgattctt cactatgtct attttatgaa aaagataata ctatggcagt tttatttaat
     9121 tttgattttg aagataaagt attatatgaa cttggtgaaa aatttctgaa gcaaactctt
     9181 aaacaagcat atgataatat gtcaataact aaatgcatat cttttggtaa aattgataca
     9241 aatataagga atatgtgtac ttcttttaag ctcgcactca atggacttaa ttataattat
     9301 gttttccctg agaaaaagat cttcactttt gcacgtatta tgttttggga agaaaaacat
     9361 tctttttcag caagattatt attaaataat ttcgataact ctctcaagtg ccttgataaa
     9421 ggaaaatgta aatctgatct gcaaaaactt gtagaaacct tgagaaatgg gaattacagc
     9481 tatcagcaaa tatacaacac attaataaat tgtatttcaa tagttgaaga ttttatatgc
     9541 gcacaaattg gcgaagcaat agatcttgat caaggtttta ggaatacagc aaatattctt
     9601 gagtttgaaa gctggatgaa aggaataatt gatgcgacaa ttgatgattt agctaatatt
     9661 gaatgtagta gcactaattt agtaaaaaaa gctcaagctt ttatagaaca aaatatccag
     9721 aatacacaat tatctttgga gtatattgct aatgaattag gtgtaagcgc taaacaccta
     9781 agtaaaattt tcaaagataa aaccggaatt aattatatag attatcttac aaatcttaag
     9841 ctaagccatt gtcgcaactt attaattaac acaaatttaa aagtggagga aatatcagat
     9901 attatgggat attccacgtc ccagtatttt attagtagat ttaaaatgat gtttgggtgc
     9961 actccaaaaa aatataggga aaagtattct agatagtggc attgaatagt acatacgtta
    10021 tttatcaatt tttcgaatat gtatcccata ctacacaatt acttccattg ccatgtttag
    10081 tactaagaaa ctatagtatt actacttcac aaaacatgta taaagaaatt tatgtgtgtt
    10141 ttcactgaat tcatatgaaa gccataatga agttgtatgt ctacatccaa gctttaccaa
    10201 tttcctcatt ggctcacaag aggaaattgg ttttttattg gtatattctg gaagttacta
    10261 ttttatcaat gtccaatcaa actaaagttt atttggacac ttcgaaaatc cggggaaatg
    10321 tctaaataaa taagataaat aagttttttt agacacaaaa gtgtctaacc ccatttgata
    10381 tgctttttta gacactttat aatgaaaata taaattaaaa aataaagggg aatttaatat
    10441 gtcaatatat ggttacataa gaattagcac taataaacaa aaaacagata gacaatggga
    10501 agcattaaaa agttatgaga aggaaaataa aatacaattt gatgcaatat ttgaagataa
    10561 acaatctgga aaagacttta atagatctca atataaagct ttaaaaaata gcataaaaaa
    10621 aggtgacgtt ttagttgtaa aagaattgga taggtttggt agaaattatg aggagataaa
    10681 acaagaatta gcttacttcc aaagtatggg tataaaggta ataatattag accttcctgt
    10741 tttaaacgtt gaagatgaaa ctctttcttc cttattaaat aatcttatta ttgaattact
    10801 ctcatacata gctcagaagg aacgcgaaaa gatacaagat agagtcaagg aagggttaca
    10861 ggttgccagg aacaaaggtg taaaacttgg aagacctgaa agaaagcttc ctgataattt
    10921 tgaaaagtat tataaaagat ggaaggctga tgaaatcaca gcagttgagt ttgctaaact
    10981 tataggtgtt agtagagcta ctatatataa ttatattaat gaatatgaat gcaagtcaaa
    11041 ggagtaaagg tttgcgatat cttactctta attctctgta actattatta atactgcact
    11101 cattgaaaaa gaagaagcct ttgatgggtt ttatagggtt tgcacaaacc tctaggatga
    11161 tgtttccgaa attatcaaga attatcaatg taaataataa aagatgggaa atagaagaat
    11221 gtcttataat catgaaaagt gagttcaaat caagacctgt atatttgagt aattatgata
    11281 gaatagaagc acattttatt acatgcttta tatcactaat tatatataga ctgttggaaa
    11341 gaaacttgat gagaaattta catgctctga agttatagat gaattgagag aaatgaactt
    11401 caaaaaatta acggtgaagg ttacgaacca ctatatacaa gaacagattt tacagatgct
    11461 ttgcttgaag attttggctt tcatactgat taccagatcg ccacttcaaa acaaatgaaa
    11521 aaaattttaa agccacaaaa aggcaatgac attgtgtact tttttataaa tgttaagagc
    11581 tttaattatc tgttataata gaattcataa aagttcctct tttcattggt attattggat
    11641 tttacctatt tctgttatat caggggaact gggacttttc aaaattttag acagagggga
    11701 tgcatctaaa gtttttgaag aaatatatac caggggctat cgttacggta cttagagctt
    11761 ttaaattcag ctacgggcag aatcaaactc gcttaataat atgaatgaaa gatttatagt
    11821 tatttgctca aatactaatt ttgctcttcc gactctcaga ttaaaaggct ctacagtacc
    11881 ttcactaatg cttcttccaa taatttcttc aaaaataaaa tattcaccct gatatgtata
    11941 ttaatgttta gccccacatt ataaatggga aagttgagta attttaataa aaaattaatg
    12001 taaaaataat aaggacggag gtgctagtat gcaaggcaat tgcaagaata tagagttaag
    12061 cgatagggat tggaactaca taatacttag accaggaagg ttattatgcc tgaaatgttt
    12121 gagtgggggt ggatgtcttc cttttatgga aaaggagaag cttaagggaa aacttgatgc
    12181 gataaaggat gatccacaga tccatattag gctggagact tcctttgatg aaattggtgc
    12241 tagaactaca agattttttc agcaaagtgt tgccgagcgc aagagggatc ttgatgtatt
    12301 gcaaaagctt ggactttcgc ctggagatgt aaggattgcc cgtgatcttt atgttctgat
    12361 cggggaaagg attaaagatg tctatgacat ttgtgggtat gggggaagta atagcgaaaa
    12421 gtggccagcc tgccctttag cccatgagga atatttttca aaaggtactg aggggatgaa
    12481 caaactaaaa gataaggata aaatgagctc ctggaagaag atatcttgtg atgaaattga
    12541 acaggcaaat agaattgtta ttagagctca tcatttgcta tgcctttttt gctatattgc
    12601 aagggacgat ttcggaggtg aatatgtgcc acttgctgaa gacaatcttt atgaggtttg
    12661 gattaagatg agggagaatc tagatatacc tgtaacagta attgaaggtc ctggagattg
    12721 catgatttgc ccaccatgtc atgggtttga taatgagaga aaaatttgtt ttgttgcatg
    12781 ccatttaaga gatcgcaaaa aagatactga tacactgcaa aaacttgatt tattgcctgg
    12841 cgagaccata cctgctaatg aagttataaa gcgtgtttat gagaaaattc ccgataattc
    12901 atggatatgt gcgtatgaat atgagtccgc tccacaatgg agaagttgcg gtggtcatga
    12961 aaggtataaa ataggcctgg agagggggtt ttttgtttct aaagaataac ttaaacttta
    13021 ggaaacgcag cgggaatcct cctcatttga atgaagcaga tggaagctgt ggtttcgata
    13081 cgaaacttta catcacttct ataaaatgca ttgcctgaaa acaaatgttc tgatacaata
    13141 taatcagagg tcaaaagtcg taccttgata actgaatata tggggttgca gcaagtaatc
    13201 ctttgctgcg taaaatattc aaggtaatga gcccaaagta cctgtgtaga gtaggataac
    13261 acctacccag cagtagtggt gttggacacg ccgtaggtgt acctgttttc agggaaccag
    13321 aagctcgtca ttttaatgac catgtagttc acaacaagtt tacatgattt gtttattgac
    13381 tcatgtaata ttttgtcgag atatatgaaa aatattaatg gagattcatc ttggagagaa
    13441 tgattgggtg aggatagaaa acgaacagga gatttcgctt gttacattgc ggctatccta
    13501 gctataaagt caaggtgaaa ttacatggat aaacgtggtt taagtaccca ggcctcaacc
    13561 tggacatacc ttgttgacga ccaattagaa caattcggaa taaacatagc ctgtcttaac
    13621 tctatttatg ccactgccaa tctaccctta tggctattat gagctttgta taacctgttc
    13681 ataggcaaaa ggcgagaaag caagctgtga tctgtcttct catttgatat aaaatgagaa
    13741 tctatgattt agcgcatatt tgaatttagt atatgccaat ccaggttttg tgattgtatt
    13801 tcagaatttt tgcttaaaca tgcattgcga tattgggaag gtgtcctatg tttatacttt
    13861 ttatatgtag tagcaaaatg ctggctggag gaaaacccca tctcaaatgc aatatcagtt
    13921 atcgatagat tagtgttgct taaaagctct tcagcccgat ttatcctggc tctcataacg
    13981 taatcagaag gagacagacc tttgtgcaag gtaaataaat attttaattt tctgggtgat
    14041 atcgattttg agtatgcaaa tttttcaaag tcaattattt cgtttggatt tttatcaata
    14101 tacgtagcaa gttcattaat atagtttggt attttgttat gttctgcaat acaacttgcg
    14161 tagatcctgt taaataagat taatattgat gccttgtaga aagctgaatg taagccataa
    14221 tcttgcatat taaaattaat aatatcatcg aaaagtgttt tcaagccatt cctaatagaa
    14281 aaaatacgct gcttaccttg caaaatcatt tttaactttt gagtatcaca ttcagtcatg
    14341 ccaagaaagg ataaatcttt aggtgaaatg tgaaaaacaa tattgtagta gacctttctc
    14401 tcggaaatgt atttcccgct gccatgaagc tctccaggtc gcacaatcag gatttgtcca
    14461 cctgaaattt tatattcttc gccattaatt ttatattttt gttcacctgt ataatgatat
    14521 attatttcaa aacaataatt gtgagtatgg tattctatgg atttttcgga ttcttgaatt
    14581 acggttcttc ctactttagc aatttcgtcc aagtttatat tctcaaaagt tattatttta
    14641 acctcgttat gagacaaata gcttcaccta ctttttagga taattagatg acactgtaat
    14701 attagtataa aactaaaata atgtcaataa caatgacttt gctttattct actaatgctt
    14761 ctttccaata ttttcttcac tctatttaaa tgtactcttt tagtagcagt taaaaacaat
    14821 gtcaataaaa ttgcattact gatttttgaa cgtataattt aataatcaaa gatataatga
    14881 aaatatgcta ctatatataa cgcaaaaaat gaactatatt attaagattt tgtgcactta
    14941 tgttatctta acgactcaat gaataatata aaaatacaat ttacttcttg ttgaatcatt
    15001 tttgcgttat atttaataat tatataaaga aattaacatt ttttaacaaa gggggtaaat
    15061 aactatggaa aagatgattc ccacttggta ttatcaacaa tttgatgcag attatagttc
    15121 ggagtttccc ggtgaagggt atggaggctg gaaaaatact agtttgccaa taaatcctga
    15181 caaaacagct atcgtactaa tgcatgcgtg ggatactggc acaattgaac aatatccagg
    15241 ttggtttcgt gctgtagaat atataccacg tgccacacat atttgtgata atttgctgcc
    15301 tcactttctt aaaaaagtac gctccagcaa tataagactt attcatatag catcttcaaa
    15361 tcaaattgta aatgattatc ctggctatgc taaaacttta gaaatatgtg gagaagatac
    15421 atttggatat gagcgggtag aagaagatga aactattaaa aaacttaatt attttcgtgg
    15481 agaaaatgta tttgttggtt cacataataa agaagatgta gcaagaggta tgaaacatct
    15541 tgacttttat cctacggcac gtcctttaga taatgaagaa attgtatgca catcagaaca
    15601 acttctaaat ctttgtaaaa agtatggtat taatcatctg atttatacag gttttgccat
    15661 taatatgtgt cttactgtat caccgggtgg atttgtagat atgtcaagat atggcattat
    15721 gtgttcggca atcaaagaac ttgttaccgc tgtagaaaat aaagagtcct gtcgtaatga
    15781 agcaaacaaa aattatgggt tatggtatat agctatttta tccgggtttg tttatgaaat
    15841 gggtgatata gaaaaatatc ttttagactg atttattaag ctgttttatt tgtataacaa
    15901 tactgtatat tcagaatatg cagtattatt ataaataaaa tttattagga ggatttaaaa
    15961 tgaaaaacac gaaactgatt cgcatccttg tattaaccct ggtatctatg atgctgcttt
    16021 cagcatgttc cagcaaaact ggaactaatt catcatcttc cagtacaaac agcaaagaga
    16081 atacatcagc tacttcaact ccaaaggttg gagattctca aaaatttgac cctaacaatg
    16141 taacgcggaa aaatccattt gaaaagttca acccagttat cgaattgact actgtccgtt
    16201 tcgaaatgcc ttttctcatt tttgagaatg gtgacaatat cgatgtaaat gattggacaa
    16261 gggcttacga aaatgaactt ggtattaagg taaaaaacct ttgggtcgtt gattcatctc
    16321 aacttactca aaagactgat cttatgattg cttctggtga tcttgccgat gtttttcaag
    16381 ttagtgatat tcaattccaa cagcttatag agaataattt aattgaagac ataagtcagg
    16441 cttatcttga atacatatct gatgatgcaa aaatgatttt aactgaaggt ggcccatatg
    16501 ccctggatgc tgcaatccgt aatggtaagc ttatgggaat accttttact ggcaaccaat
    16561 tggagagtat atcccttacc tggattagaa ctgattggtt aaaagcattg aatttgtctg
    16621 ttcctacgac tcaaaaagaa cttgaagatg tgttaatcgc ttttgctact aaagatcctg
    16681 atggtaatag taaggctgat actcatagtt tggttcttac aaaggatgct tttaaaactt
    16741 catctcacca ggacgcatta ttctattcat ataatgctta tcccaacacc tggtatgatg
    16801 atggttccgg caatcttata tggggcggta ttcaacctca aattaaagat ggccttgcag
    16861 cattcaaacg gctctatgat gctggcgcta taaatcggga atttactaca atagatttag
    16921 cgaaggctaa agaagctgtt gtggctgata aaattggtat gatttatgaa ggttattatg
    16981 gttccggagg aatcctccaa ccacatgtgg aagctacaaa gaagactgat atatggcagg
    17041 cttatccaat accttctagt gacggtaaag ctgttaaatt gcctcatgat actgcaacct
    17101 atgggttcca tgtagtcaga aaaggcttca aatacccgga agctgctata aaaatgctaa
    17161 atatgtggat tcaggagttt tatattaata caactgataa tgacagatac ttgcagctta
    17221 ttcaaaatga aacaggaact aatgcaaaat ggaatttatc tctttgtcag gcttatcgtc
    17281 cactaaacaa cataaacaac ttcagaaatt tagaaaaatg gaaagaaact ggtaacaaag
    17341 aatattatga taaagttact tatttaggta agagcttcta taatggtggt attagattca
    17401 aggagaacgg tgaaatatcg cagtggttct ggtgggttat gcattgtcct gaatatgaaa
    17461 atccagcaat gaggattaca agtaaaaata ttcagaataa gaaccttcgg cctaatttgt
    17521 tctatggtgt acctactcct actatgactg agaaatggaa actgctttgt gataaacagg
    17581 ctcaagctta tcaaagcatt atcgcaggcg gatctcttga tgactttgat aagtttgtaa
    17641 atgagtggaa atcattgggc ggggatcaaa ttgtaaaaga agttaatgtt tggtatcaag
    17701 atcacaaaaa tgccgccgat aatgtatatg taaaataggt taacacttga tttgatgaat
    17761 gtgttaggct ataagaggac gttaagcaat aagatgttta cgtcctctta aaacaaataa
    17821 ataatattaa atggtaaagg agagtaatca gtggcaacaa aagacttaat aaaatttaaa
    17881 cggcaaatac ctcttcattt gatgatggtg ccagcaatag tactggttat aatattttct
    17941 tatataccaa tggcaggcat tatcattgct tttcagaagt ttgttcctgc aaaaggctta
    18001 tttggacccc aacagtgggt tggacttgat aattttaagt atatcctatc tatgcctgat
    18061 acagggagag ttttcagaaa cactatatat atagctgtca tgaaaatgat tgcaggtcag
    18121 ctggttccta tagctatagc aatacttttg aatgaaatcc gaaatcgtag ttatcaacga
    18181 tggatacaga cttttattta tttgccgaat tttttatcat gggttatatt ggctggaatt
    18241 tttattaata ttttatctgt aaaagggatg gtaaatcaat taattgcttt tttgggtgga
    18301 aagcctatat cctttattgg taacaaaata atattccctt atacacttgt tgctacagat
    18361 gtatggaaaa attttggttt taatagcata gtatacctcg cagctataac tggaatcgat
    18421 ccaagtttat atgaagctgc tacagttgat ggtgctaacc gtatgagacg tatgtttcat
    18481 ataacacttt ttggaatgtt gccaatcatt gtcctgttgg caacaattaa tctagggagt
    18541 attttgaatg cgggtttcga acaagttttt aacttatata gtccaaccgt atatgaaaca
    18601 ggtgacataa tagatactat ggtttaccgc ttaggttttg acaatgcaca atatagcata
    18661 gcaactgcta tgggattatt gaaatcagtt gtatcaacta ttttgatttc tgtatcatat
    18721 tatttagcgt atagatttgc caactataga gtattttagg aaggtgtgtt aaatgtctaa
    18781 agcttctaaa aaggggaaaa gaactaagca taatataata cccacaggtt cacttttatt
    18841 tgatgtgcta aaccatatat ttcttggtat agttgcatta tcttgtattt taccattaat
    18901 tcatgtactt gcaatatcct ttagtgtaag ctgggcagct caagggggaa tagtatattt
    18961 ttggccggta ggatttaatt tagaatctta taaatttata ttgaaaaatg gcaaattcat
    19021 aactgcatta atagtttccc ttaaaagagt aactttggga gtagcaataa atttgttttt
    19081 aataatttta actgcttacc ccttgtctaa agaagaacat gattttaaag gccgaacttt
    19141 gtatgcctgg tttctattta taactatttt atttagtggg gggttaattc ccagctacat
    19201 gaatatacga tcattaggac tattagataa tatattggca cttatactac ctggggccgt
    19261 accggtgttt agcgtagttt tgcttttaaa cttttttcgt caacttccaa agggagtcga
    19321 agaatctgca tttgtcgatg gtgccagttt ttggactaca ttgtggaaaa tatgcgttcc
    19381 aatgtcacta ccttcaatcg ctacaattgc tctgttttca gctgtagctc attggaactc
    19441 atggtttgat ggaattattt atatgaatag aacggataaa tatccattgc agtcatattt
    19501 gcaaaccata ataatgatgg caagttcatc taatgaattc attcgagcta ctcccgaaat
    19561 ggcagaattg ctaaagaaaa ttaacgactc tacactaaaa gcagcacaaa tatttctcgg
    19621 agctttgcca ataatattag tatatccatt tttacagcgt ttttttatga aaggaattgt
    19681 tattggatct attaaaggtt agcactgact atagtaagcg atggaacacc taccgtctaa
    19741 ttacttgttt aaagtatatc aacggaaggc tgtccggaca attccggtaa acgtgtccgg
    19801 attcatccga aatacgcaca catatatttg catttttaat ttttatagtg agaaaatatc
    19861 attaatgaac ttatcttctc agaaattata gctcatagaa acataggaac ggttcgaaac
    19921 cactgaaaaa catattatgg gtagtgttaa ctgcatacca cctatgctcc gcctagaggt
    19981 aggagctttc tgctcaattt aactagtgca aaaagcatta acaggctacc tctgtagttc
    20041 ctacggtttt ttatatgata ttctcaatcc ttcatatctt atgttcttag cagcattgta
    20101 acctctatca ataacactcc cacatgattt acatatccat tttccatcta acagaaggca
    20161 aatatataag agaagaccta tcgttagaac tttagcaaca cctgctttta gcagtatgta
    20221 tgtggtataa gtactatact gatgtttaac ttttgcctaa ttcagatttt ccttagtaat
    20281 aactgccaac ctaggcttgg cagttattac cttgtgcagc ctactctcta cttacaatgg
    20341 caattatgta attataaata ttcttattat aatcgatcat aaataggagc tcttgtattt
    20401 attgtaaaaa caaaatctcc tgttatgctg tagatctctg cttgttcaaa ggcaatttgt
    20461 agttttatcc attcatccct tttgcaaatt tcttcaaaat ttttcccacg ccacctaagt
    20521 tgaattgcta gatgatcacc ttgtatcgga tcacaatcat cgtatgcaaa accttcaaca
    20581 acaccattat tatttaaaac ttgtgctttg acacgaccaa atggagctga aagattaagt
    20641 gtcaattctg atgatctaat ctttagattc tgtgttgtca tctcagctgc gccttttcct
    20701 gaatctatac aaacaaagcg atccttcttc atgcgacctg tgacaacttg tttagctgcc
    20761 tcaccatcag ttgaaacaaa atttgatggg ccaccatgct ctgttttaca tgcattagca
    20821 tggaaaatta gttcgtcgcc taactccatc atagaaaaca cataactact acctcctcca
    20881 agttcaccac ggttacgttg tttcataact ggtgcatggg tacgattcca ggtaagacca
    20941 tcatacgagt acgcatattc tgttaatact ggaccatcca tgatacgatt gtacggcatt
    21001 atctgtttcc aaagcattcc tatgaaaata tcacctggtt cataatagaa atgtggcata
    21061 ccataaaggt gggtagtggg ttcatcggaa ggtaaaggat gaataatggc cctgggcata
    21121 gaccagcttt ttagatcacg actttctaca agacatatgc ggcgttctcc ccaacgtcgc
    21181 cgagatgttg tttggtagtt tccagtcaca ggattgtata gcaatgatgt atagcaatct
    21241 acatagcttg gtattacttt tacatcattt ggtttcttcc aatgaagagc gtcagatgat
    21301 actagcatca ctggagcttc ctctataaga cctttcggtg attcaccata tgtagcacat
    21361 acagctttat acctataatc cggggttttt tcatatttat caaaatatcc atatattccg
    21421 atgtattcat atgtatgtcc gagtgcgtat ggcatatctg gataccttgg atgtagatga
    21481 ttataattca aaaaagaaat tccatcctca ctatatgcag tacaaaccat tagattttca
    21541 tctatgaatt tcttgtcttt tggaacagta agataataaa gaaaatatcc accatctgga
    21601 gctggaataa cactttctcc aagtataccc cgcagagaac caggttcatt aaaagcattg
    21661 ctaaaatccc ataccggttc tttaaatact cgtcgtgtat tatcagtgcg atttactata
    21721 aaatcatcaa agaacataat ctttttcatt tccatacctc tctttcgttt gtttttagtt
    21781 attgttggta tctatattac tcaggacata attctggtat tctccaggtg taagtcctgt
    21841 tatactctta aatactttta taaaataact atcacttgag aaccctactt gaccagaaat
    21901 agaatttatt cttgtttcat tattattaag caataatttt gctttttcaa ctctatattt
    21961 attaagataa ttaataaagg ttgtattata ttcatgtttt ataatataac aaaaatattg
    22021 tttagatata cctaaataat ctgatacatc atcaagagaa attagcttat caaaattttt
    22081 ttcaataaat tgattcattt tcttaaggta gatatgtata cctgactttt cttcactatt
    22141 gcgattagag gaggacctaa aacatttctc aaaaaaactt ctcatttttg aagaatcaag
    22201 cttgtcatta ctttcaatat agtcaatgaa ttcattaata tatacggaag catcttcttt
    22261 gaagtgattg atcatatatg caatcattgc acaaatcctc ttattgaatt ctatagcagg
    22321 taaccctgat ggtaaataat cgttaataat ttttgaaaat tttttttcat cgaatttgac
    22381 tagacaatat ttcaattcat ctacctgctc cgattctaat ggtatgtatc ctaaagccat
    22441 attcaatatt tcattatatc ctacaatccc atcaatattt agaaaatttg cgagtatgca
    22501 tgtaaacatt gcttcactat atgcacggtg caattctttg agcatacggg tgttttggca
    22561 aattgaaacg gtacatcctt tataagccaa tttgtcataa aaatcttcct gcaaaagctt
    22621 tttaagatca ttcatgcttg tttcatatgg aaatacacaa attaatttat cattattatt
    22681 aaagtattca ctattaggta acgacatgtt taatatttct ttgtactggt cactctcgaa
    22741 aatttcatca ttttttaaca ataatacagc ataagtttgt tgctttgaca ttgaagttat
    22801 ttgatcactt actacttcta tcacttcctg ctcttgcccc atcttaagat gatttagcag
    22861 agttgcgcga tgtaaaatgt aatgaatagt ttttaaatct gagttagctt gttgatattt
    22921 gttttctata ttattaaatg ctacacttat gaattcaaat tcatcttttt gaaaagattt
    22981 gtagccaagt tttatactta catccctcat aactttatgc accggtccat ataagacatt
    23041 tgataacttt attgctgcta tagagagtat tgccataatt acaatagtga tatagaaaat
    23101 aagatggatt aatttatcaa cccaagcata taatttttcg gtatttatat cagcgacaag
    23161 atacaatgtt gttccactga tggggaatgc gctcataact acgctctctt tttggtattt
    23221 accgtcttcg ataaggcttt tatttatatt agggggaagt cgcgtggcaa tcaaatcatt
    23281 atttgaatga taaataatac tcccttccgc atttattatg taacatgtat aagcatctcc
    23341 tgcgttgcta gtattgttta ataattcttt catcaaaact cccgtattaa ggaaaataaa
    23401 agacactaca tcagatttat tattcgcacc tacaggatgt atgataacag aaaaatactc
    23461 aatctgttca ctgtactgaa tcaaacgtgc cggagatata tatatctgtt tattctcgtt
    23521 agctttttgc acttctaatg gtagccaact gtagcgtgga tttgtaatat ctgtaatacc
    23581 atagtttgat tccataatta taccattaga tgggtcatat aaacatactc ttaccttatt
    23641 tcctgttata gaagctaatt gcccaagtgt atcaagataa cgttttttag taagtatacc
    23701 tatgttttcc aggccattat ttaaattttc aaatattcct ggtgtactgg ctatataata
    23761 taaaatatcc tgcatttgtg ttattgatga cgatatatta ttagtacttg aaaccaattc
    23821 aatataacta atatcacgta tgtaatttat aagttcatat tttatctttg aactaaaata
    23881 taaattgaac actatatatg gtaaaaggct taataacagc attattgcaa gtattttgta
    23941 aaaaaaccgt ttagaaatcc tactttttaa accatttttc atgtagcatt ttcctctcta
    24001 taaaatctaa aataattttc aagtttatta ataaacttaa aaatcacttt agataaatca
    24061 tacacttaat taagcataaa ccaatttcaa tagcagttca atttattcca caatttttta
    24121 tatgtattaa gtagatcctg ttcaaccttt tattgaccct acaactagtc cttttacaaa
    24181 atacttttgt gcaaatggat agaacactaa tacaggcaat gttgcaataa aaacagaagc
    24241 tttcttaaca ttatcagtag gaatgttgat aagtaattct tccatatcct gaatactttc
    24301 aacggaagtc cagtcaaatt gtgccaacat ctgtcttacg accatttgta ctggccattg
    24361 ttcacggctg ttaagataaa ttgagggagt aaaccaagaa ttccattgcc caactgcata
    24421 aaatagccca atggtagcaa ttgcaggaag tgatacaggt agcattacct tgacaagtat
    24481 ttgcacatca tttgctccat ctagtaatgc tgcctcctca agactctcag gtatctcgcg
    24541 aaagaaattt ctcattaaga atacattcca agaacctacc attccaggga taacacatgc
    24601 ccatagtgaa ccatatagac caataaactt tacgacaaga aacgttggaa taagtcctcc
    24661 acctattatc atagggatat aaacaaagta agttagaatt ttcctatacg gtagatattg
    24721 tttggaaagc gtgtaagctg ccatagttgt cgctataaga gaaaggcttc ctccaattac
    24781 taccaaaaaa aatgaatttt taaaagcttt aataataagt ttggagctta ctaataagta
    24841 cccatacgca tcaaaagtca cttgttttgg tataattgaa taccattctc ccgtcatagc
    24901 tgatctggga gtaattgact gcaaaaatat aagaagaaat gggataataa ttgaaaatgt
    24961 taaaattata actgtagtat aagcaattcc tattaatact atatctgatt ttgaattttt
    25021 atatttcaag gatatcaacc tcgtttccat actaattagc tgtaaaaata tcaaatgttc
    25081 ttaaatttag gatgtctcta gaataatgtt tctcttccca ttttcccggc aaaaaagtta
    25141 gttattataa gtaatgtaaa acctattgct gtcttaaaaa ggcctactgc agacgaatag
    25201 gaatatttta attcttgtat tcctgacctg tatacaaatg tatctattat atcacctgtt
    25261 tcaaatacca atggtgaata taacatcaat acctgttcga atcctgcatt aagtatacca
    25321 cccatactca tgattagtag tattgacatt aagttatata gtgaaggtag actgatataa
    25381 agtatttgtt ggaatcgtgt agctccatct atcacagaac tttcatataa ttgtggatca
    25441 attccagaaa gtgtagccaa gtatataata ctgctccaac ctactccctg ccatactgaa
    25501 ctaaacacta gtacccatat aaagtaatca ttattagtta agaaatcaac aggttgacct
    25561 ccaagcgcaa ttataaactg attaatagtt ccgttcgcag gagaaagtaa gcttgttata
    25621 attcctgata atattaccca ggataggaag tgtggaagat aggaaactgt ttgcactatc
    25681 ttcttaaact taaggttacg tatctcatta agaaataaag ctaatataat aggtgctgga
    25741 aatccgaata atatttttaa taaacttatt ctgacagtgt ttatcattaa ccttttaaag
    25801 taaaaagacg agataaagtt tttaaattgc tttaaaccaa cccatgtagc ttttgagaac
    25861 ataccttcta cgccaagatg aggtttatag tcttgaaagg ctattacaac accatacatt
    25921 gggaaatagt taaatataag aaataaaact atagttggca gtataaggag gtaaaggaac
    25981 ttgtacctta taatattgtg gatgaaggct ttaggctttc tactagattg tgctagagta
    26041 ttcatatttt tcacaaatta aactccttta cggtgataat atcgatattt aatttattat
    26101 tatatagttt ataaatttat gtgtaatgtg cgttaagggc ttacttgccc ttaacgccat
    26161 ttattaataa acatattatc aatatattag tattacttgg aacctaacca tgctttatat
    26221 tgtaaattta tatcttcaag atactgttca ccacccatgg ttttccatcg agacataata
    26281 ttatcccact cggagacagg aatttctccg accaaaacct ttagacgctg ttcttgtaga
    26341 aaagtatcca tatcagctaa tttagcttca gatttaaatg cttttgtatc atatgcaaca
    26401 agcttatcag gtgggtcata tccaggcaat gtcttgagaa catcagctaa ttcgcgatac
    26461 agtttcaaat ttggggcagg accagcaaat aaaccaaact gaagttgagg tattgacaat
    26521 tggggtaaat agactccata atataatttg tttccatcct ctccaagtat gctgtcaggt
    26581 tgaccatcga ttagtttata tgatatatcc tcaatacccc aagtacaaag aatttgtcct
    26641 tcatttgtat atagataatt caaaaatttc attgctgctt ccggattctt tgatgatgta
    26701 gttataactg gtgaattagg ggtggcattt tcgttacgat aagcattagc tgcttccggc
    26761 ttgcctttaa tacaaattgg caaataatta gcttctggta ctgatttccg taatgtaaca
    26821 ataccagcaa taggtgctga ataccatgta gctaatgcac caactttatt gcttgttacc
    26881 caatcattac gttgatcatc agctgacgta aacacttctg gtggtaaata tccagcttta
    26941 taccatcgag ctaaatcttc taatagtaat ttataattag ggtgtaattc agcaatctgc
    27001 agctttccac tttcatcaag ccatctctcc cagcctgtag ggcaataaaa cattgacatt
    27061 attgttacaa tatttgctcc ccatacacct gcagataagc catatgtgtc atcctttccg
    27121 gtattattag gatcttttgc ctttacagct tccaggtagg cttcaaaacc ttcaagggta
    27181 gtaggaatat ctatatttag ctttgaaagc caatctgtac gaatctgtaa tgtctgacca
    27241 cgagggacaa gtgtaggcca tggcattacg agatcctttc cttgatattt tgactcttca
    27301 cgcgctttag ctataaattc atatttttca caagccgtta aataatctgg agctttgttt
    27361 tttataagct ctgttaagtc aataattaca ccatcattaa aatactcaat tgatttttgt
    27421 ctattaatac ggggaataat atctacctga tctcctgatg cgataagcat atttacacga
    27481 ttttgatatt gatctatagg gtaaaaaaca ggtcgtatat ctacattaag gacttctaat
    27541 attttattat gtacttgctt tgatgcatcc gttttcaact gtgcactata gccttcatca
    27601 acgttaggcc atactagcac tacaggtgtt tcttttttgg aatgaacttc tgatgtggtt
    27661 gttttctgtt gttctgtttt ttgtacctga catgccgaga ataaactaaa catcacagaa
    27721 atcataacta ctaaaataat taccttactt ttaactctca tgactactcc tcctaataat
    27781 ttattctata cataacgcaa aagcgcttat gccaaataaa attacgaaat taaaatgagt
    27841 ttgtatataa aatgtgcatc agtatagtat attgaaattt aacagactgg tttataacta
    27901 ttataggata taataaaaag gatttctata aaaaaattgt ccgtattgtc tttatgaatt
    27961 ctgtagtatc attaagtacg gccaggataa gcttggaata agctttacat aggatactat
    28021 atgcatatac aatgttttag taataatatc ttcatgtata aaaatatgaa tatattacta
    28081 ctaaatagaa tgctagagag ctagaaaaaa taataattag tgttttttat agtccacttg
    28141 tcaactaata ttaaaagggc ctgactattt aaatagagga atcaaccatg agttctttat
    28201 actttattta aactaaaagt ctaacttgat agccactata cgctctaaag gattatttga
    28261 atcttttcat ctaataattg gatttaggga ccacagcttt aatctgctcc attaaatcca
    28321 ttaaaatgaa taggattctc gctgtatttc ctaaacacct ttttgtaaca ttcaaattat
    28381 tgtgatttaa agtttccata attaaggctg ggttaaatcc ctttttccag tcgtggtgcc
    28441 cccaggcctt tcgcaaagca tgcgttccga aacgttcctc tactccaatg gctgctgcag
    28501 catcttttag tatatttagt gcttgttgcc tggtaatgga tttgttatcg ccttccctag
    28561 actgaaaaat ataatcataa gtatctaagc tttctaaaag ttctccttgg gctttttgtg
    28621 cacttctatt tagtttaatc tttctttgct tctggtattt gccctctttg atttcgataa
    28681 ctttaaaatc tttattttct tttaaaacat ctctccaagt taattctaac aaatctgata
    28741 tccttaatgc cacattaatt cctactacga ataaaatata atcctgctat ttattccctt
    28801 tagataaatt ttcacagcat ttatactttt tggatcacga ataggttcaa ctgctttcat
    28861 aaaatatatt ttgtgtatca gctttaggta ctactactta tttttaataa accttatcac
    28921 aatcataggt atggtggatg ccgaaagcga aatcacaaaa cacagcagtc catcggactt
    28981 tggcgatgac aactatgatg gtgtttcgct gtattgccgt aaggatgcga aaccgatagt
    29041 gcttttcatg agaaagaacg cagaccctgc ccggtggaag gtcttggatg gagcatccga
    29101 gtcctacttc cgctcatacg cagaggctat gaacttctgc cggatgcgcg gataaatctt
    29161 tatgaaggga ggaaaacagt atgaagctga ttaacctgct ctatgcagaa atcacaacag
    29221 caccaaaggc aaaatgcatc tgcgcaaccg ttccggcgaa cctgtgactg taacaaaaga
    29281 aatccgttat accgtcagcg gcgggcagtc caacatccat accaacggtt ataatgatct
    29341 gtccgaatac tgccgtactg tctgatatac ctatcgttcc ggcaaagtgg tatgaacaag
    29401 aataccccaa atcaaaattt aaggaggaaa atattatgac ttgttaccca cttacaaaaa
    29461 cagaacgcac cggtaaaatt atgaagccgt tgcaaaccat gaacagcgag gagttcattc
    29521 gtgaccgcta cgatttatat ctcacggatg aactaatcta caacgatgat ggcaaattca
    29581 aacgtcgcta ccgtctccat tccaagcaag cgcaccgcat ggaagattat ttggcatatg
    29641 acatcctatg tcctaagtgc agccaccgat ttggtaggag caagtgggat aacccatgta
    29701 cttgggaatg gtgtgtaagg ccacgctgat atcatcggcc agctgctttc tctgtttgcc
    29761 ggtgatgtta tatggtattg tcattttcat ttgtcttcgc ctcctttcta cataaatcct
    29821 cttgatagat aacactaagg ctggaaccgt tatcccgatt caccataatg ctgccaatgt
    29881 catcgactca caccactgtg ccttttgtgc caataggcga agcttgtggg tcgttcattc
    29941 gcaaaagttc gacgcggcat cctatgggat attgtttacg cacacgctct gaagtgttct
    30001 tataatctct gtccattgca ctacctcctt gtttttggta ggtacatatt cgctctaaag
    30061 gcgcaggata gcaagtcatt tagggaagat gtatcagaaa gttcaaaatt tatcgtgtga
    30121 cttcctctca taatatgcga aactgtattg tattttgcga gagtatatgc tattctatat
    30181 ggtagaatat tgtatacaca aggctggagg cgtattatgg ctacctcata taacaaacta
    30241 tggaaactgc tcatagataa aaaaatgagc aaagccgact tgcgaaaagc gtccggcgtg
    30301 tctcctaata ccatgacaaa actacgccgg gatgagccgg tgatgctcag tgtgttagac
    30361 aagatatgta aagtgctgga tgtcaactat ggggacatcg ttgattacat tccagacata
    30421 gagaacgagt aaaatacatg acaggaaggg tatgcaatga acagaataga cattattaac
    30481 cgaattggtg acaagtatca tgtagggaat acagagaacg gagaatttag ctacgtacaa
    30541 gcactgaaat ctgtgggcaa agacattaac gactatcaga aagccacaac gggtacgaag
    30601 caccagtttc ttgatataag attcgaaaac gataggcttg ttgttcttgt tgaatgcaaa
    30661 aacaaattta gcaagtggcc gaaagataga atccaagcac agttgcagga ctatgtgcgc
    30721 ttcgaaaagg cttattctga taagaaaatt gttgctattt tagcagaaac cgatggggat
    30781 gacatttggg tttggtacgg acaatccgtt attatcgatg atgaacatcg caatgatgaa
    30841 gaaaccacac ttaagacatt tgaagaatat gagaatcttt gctttggcaa agttaacgat
    30901 aaaatcaaag tggtagattc gataaaaaca ttaaacgaaa tgctttattc tgatggaatt
    30961 aatgagaaat taagaagtca gtttgtaggc acctgcctcc ttgctctaaa aaatggactt
    31021 acatataagg acgtcaagga gacaataaat ccttctacag ggaaaaagct ttctccggag
    31081 caggtcgttt tacgtggcat aaaagacatt ttagaagggc tgcttactaa gagtggaagt
    31141 ataaacaaag ccagcaaact tgcgattctt aacaacaaag tattggatga tcaagatatt
    31201 gcaagtctga catatgcaga attgaaagct atccttagct atatcgatga aaatgttgtt
    31261 ccttatatca acgataaaaa cacagcaggt caagatttgc ttaacctgtt ctttacgacc
    31321 ttcaataagt atgtaggaaa atcagacaag aaccaggcct ttactccaga tcacatttgc
    31381 gactttatga gcaaagcagt tggtgtaaat aaaaattctc gagttttaga tccctgctgt
    31441 ggaagtggcg ctttcttagt tcgtgctatg actgacgcaa tggatgactg cgatactgaa
    31501 aaggagcgcg aaaatgttaa gcgtaatcag attttcggaa tcgaatatga ggaaggggct
    31561 tttggtcttt cttctacgaa tatgcttatt catggagatg gaaactcaaa tgtcatacaa
    31621 gattctatgt ttaaccgggc gaaatggatt gaagataaca atattaatat tgtgttgatg
    31681 aatcctcctt ataatgcaac taaaaaaaac tgcgatccgg aatacacaaa gaagtgggat
    31741 tcaaagaaaa aagaagatcc atccaaggga ttacatttcg ttgaatgggt tgcacggcac
    31801 tgcccttcta catgcaaaat ggctgtttta ttaccaatgc aggcggctat cggtaataca
    31861 ggggaggtca aaacatttaa aaagaaaatg ttggataact atacacttga tgcagttttt
    31921 tctttaccga cagaaatttt ctatccgggt gctgcagctg tagcatgttg catgatattt
    31981 gacttatctc aaaaacacga aaaagcaaat aaagaaacct tctttggata ttttaaggat
    32041 gataaattta tcaagagaaa aggtcttgga cgcattgaga agacagactc tgaaggaaat
    32101 accctttgga atagaacaga ggaacagtgg ttggatttgt acaaaaacaa aaaggttgtt
    32161 actgggttat cagtaatgca taaagtctcc tggaaagatg agtggcttgc cgaagcatat
    32221 attgaaacag actattcttc gttaacaaaa tctgattttc agcaaactat caatgattat
    32281 ctttcctatc tgataaagga aggttatgtt tatgaatctt gattttaaaa attggaagac
    32341 atttgatgtg aatgatatat tttacttgtt taatggaaag ggaataacta aagaagagat
    32401 tgaagaaaat ccaggagatt tctttgccgt acaaagtggt gctgaaaaca acggttgcat
    32461 tggacgtata gataaagatt attgtataaa taagggatac acttatacag acgagccgtg
    32521 tttaactgtt gcaagaactg ggactgcagg atttgtctca tatcagcatt atggatgcgt
    32581 ggttggagat tctgcaaaga ttttattatt aaaaaataag gagaaaagaa gcactcccat
    32641 atatttgttc cttagaacta tactaattga gctacaatat aagtatactt atggtcgaaa
    32701 ggtaactgaa aataaatatc tttccgaaaa aatacttttg ccagctatag ataaagagac
    32761 gccagattgg atgtttatgg aaaattatat ttgctctctc gattataaaa tgccaaaaac
    32821 tacagttcgg ctttcaaaag atatggattt aaagaccaaa gaatggaaag agttttatct
    32881 acataaatta tttaatgctg ggatggggaa tggcatagat gcgattttaa ccagtagtga
    32941 taaccccatg tataattacg tatcgcgtga cagtaatggg aacggagtcg taggttttgt
    33001 agatgaaatt gacggacaag aaccattccc aaaaggtgcc ctgtctcttg ctttgggagg
    33061 tagcttttta ggatcttgtt ttatacagaa agagaatttt tacacggcac aaaacgtagc
    33121 tgttttacaa gagaaatttc caatgagtaa tcatgtaaaa ctatttatcg caacactcat
    33181 aagaaacgaa agtaaaatta aatatcaagc gttcgggaga gaacttaact cacattttag
    33241 gaaggatttt acaattaaat tacctattaa acatgacaaa aatggaatcg tgtacgattc
    33301 ttcacttgaa ttttcggact tgggatacat tccaaactgg gaatggatgg aacaatatat
    33361 aaaatctttg ccttatagtg ataaatttta gctttgatat aaaggtgcta catatttgaa
    33421 agttatgaat acccgccttt tcaatgagcg gattgcttca tttatggagc gtgaattcga
    33481 ataacattat cggatagctt caggacgagc aaccgcattg gtgcagaata agattccgtt
    33541 tgaactttca ttatggggag cgggtatatc ataaacaatt tcgaggtata ttctttatcc
    33601 ttggtattaa gataacagaa aaagccccac cagagcaatc cgctcccatg gggcttcacc
    33661 ttttctggtc gctcgagttt tatcaccgcc ccgtccttga acgtgaagcg cacatgctcc
    33721 gcaccgtagg ccgtggcgaa gcccacaagg agactttcat gtattatcgt ttttcatcgt
    33781 cacataaatt taaacaccaa ctaaacacca aaggcttcgt tacagtgccg aaaagtcccg
    33841 tagtatctgg ctttctgctg tatctaagtc ttttttttca attaatcttt tacgaatttc
    33901 tccctctgct ccgccgcgga acaatactcc atgtggtaat acaatagcca tggttccact
    33961 ttgtctaaga tggaatagtc catgtagaag aaaagcaaaa tcaccttttg aatctggagg
    34021 caacacacct gcaatctcaa atcgtggatc acttaccttt aatcctgagc ggttccaatt
    34081 ctttacagaa taaggtggat tcataactac tgcatcaaat tgtacccctt cattaggtct
    34141 ttctggatct tcaggccaat cataagagag agtatcacca tttcttatag tcattttttc
    34201 agggcgaaca ccatgtagta gtagattcat acgagttaag ttataggttg cagtattctt
    34261 ttcttgtcca taataattaa gatttttctg tccatcttcg tctagatgtt tacctactgt
    34321 caataataaa gatccagaac ctactgttgg gtcatagatt gattcaattt tagatgattt
    34381 agcaacaatc tgtgccataa cttcactcac ttgatgtggc gtatagaatt ccccagcctt
    34441 ctttccagat tccattgcaa attgaccaat taaatattca tatgcgtcac ctagaatatc
    34501 tcctttttga agagcaacca tgttcaaatc agcaaaaagc attatcaatg cttgtatatt
    34561 tttactacgt tcatttaaat tgcttcccaa ggctgtatcc gttaagtcaa ttgtagaact
    34621 tgaaaataaa cctttaaaat catcattacc agattcaata gcgacagaac gttcaaaatt
    34681 atgtaaacta tctgttacct tatgtacttc aaaatcacct gtgttaatat ccattatcca
    34741 tgtttgatac aaatactctg gaagaacata atatcctaat acaccttgta tagttttatc
    34801 taaagtttca ccataagctt ctctagcctt tttatattct ttcactaact cttcttctgt
    34861 tactttgcct aaacctgcag aaaccttaaa tgcatctaag gttttatcac ttaaaaattt
    34921 ataaaacatt aaacctagca tatagtcttt atatctactg gcatccatcg agcctcgcaa
    34981 ctcattagcc ccatcccaca aacgtctttt aatttcttct gatgtaatca tagcaaccac
    35041 ctcccgttat atattaaagt ttatcagctg agtcccattt tttcttatcc agttgtaacc
    35101 tgttcattaa tttactcaat ctccgcactt ttcccactat caacccattc atctttagtc
    35161 gttggattat tgacattttt aatgccaata tttttttcaa aaaagcaaat tcattaagct
    35221 gcccttttga aacaaactgt accttgtcgg ttacaatttt tataagctcc tctgacatac
    35281 gttctaatac atgctaaaat aataatacta tatcgtgttc ccttgtagca atccctttaa
    35341 catagtattc ctctcaacca gcatcacatt ttttatcgaa taatacattg cttttggatc
    35401 tcctattata actactatat cctcagccct tgttatggct gtatatagta agttccttga
    35461 taacaacttg taattgtgaa atgatatagg aattattata actttgtttt gagagccttg
    35521 gtatttatgt acagttaagc tatatgcagg ttctaattgt tcccattctt ctgccccgta
    35581 taatacatag caatcatcat atttaactac cactttggtg aaggtgtctt cttccttttc
    35641 atcgaataaa tctataatgt cagctattat tcctgtttct ccattaaaca caccttgtac
    35701 cggttcagaa ttatcatcta ctataacttc gtactcatta tttatataat tatacttatt
    35761 tatttcgtag ttgttcttat tatgaaacac tttatcgcct gtacggaaag taaaatagcc
    35821 tatccgtaat tctcttttgt taggtgatgg tggattaacg agttcttgga ttctttcatt
    35881 catagccgca gtccctatca tacctttttt ctgcggacat aatacttgaa tgttatccat
    35941 actatactta ttaaggtagt actggacaca taataacatc cttttagcaa tcatctctgg
    36001 ttctttttct tctgcaatca caaattgtcc tccatctgaa tttgatacta tcattttgcc
    36061 atcaataaca ttagtggcat tttttattat atcgctatct agtgcttgcc gtttt
//
LOCUS       DAOSDD010000010        35789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_609_length_35789_cov_5.517854, whole genome shotgun sequence.
ACCESSION   DAOSDD010000010 DAOSDD010000000
VERSION     DAOSDD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 35789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35789
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_35789_cov_5.517854"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PK733_01695"
     CDS             <1..173
                     /locus_tag="PK733_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HOJ09291.1"
                     /translation="PDKYKYLEIVEGIVEKRGLKVDREYLHREALKWELWYNGRSPRT
                     ARQFVDWLEGTL"
     gene            405..1058
                     /locus_tag="PK733_01700"
     CDS             405..1058
                     /locus_tag="PK733_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ09292.1"
                     /translation="MGDDLNLVKKVLKGETQYFSNLVEKYENRVYKTCYRFVRNEEDA
                     LDLAQEVFIKVYNNLAGFKHSSSLSTWIYRISVNTCLNYLRKNNRLLLNFEDFNNSLN
                     YRDYKGISEDSGGCDYVSSDGNISPETHIGSDTLGSTSGNPENYVQMKEMLELLDLNL
                     ERAGNKSRKIFMYRLFHGMPFKEIGKKIGVTPEAARMNYSRTTRSLRKSFREYQKGE"
     gene            1064..2812
                     /locus_tag="PK733_01705"
     CDS             1064..2812
                     /locus_tag="PK733_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09293.1"
                     /translation="MFCNSIEEYSLLYLENEVSTKEKKLFIKHINKCDRCKRMYNLIR
                     SNYHPETKVAVKSPVVSDKINILIDRGRYPDTKTVNKVFSKKRIVLVSVIVILSVFAT
                     VNAKTIYKTYKELVNSMFVDRSLLNMSGIIHTGMYSDKDYVKSVYVKYQDRIDEYITG
                     MKTAEISKLEYDEDGENFKVQIAKGIKGNFISYGFEGYTIFHNEFFDKYTMGLAQGMQ
                     SIFNNREEYYSIDELLNAAGICPILGYVPDGYNFKSSYLVSRNQGGNIPDELGLRYQK
                     AEDESLYIALTRRRKAIDNVDVANEEKHAATNEELDINGYQAVYIEEDVESSKKDEVY
                     KTFRSVSVFLGDQAKLPILLVESASLGREELINVAGNITVNNISKNQIKAEEYFRGIK
                     DENILAYADKYLDNIKAGKTEFKFKIDKNTEFYKSGNNDTYYIMYRNADINKISSFIQ
                     LPIKLNDDFLNRFQRSDVQVSGQPYNEQKSVYLNLKNENNNTFSLFSDKMRRIGEELG
                     LTDQLIAKMSGIQYEAEPLFSSSGHLYYLIESTKKTWILQTLYEENDLVGSYNFSIDK
                     KILSDREAIIKFINSL"
     gene            2843..3043
                     /locus_tag="PK733_01710"
     CDS             2843..3043
                     /locus_tag="PK733_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09294.1"
                     /translation="MEILEILGMVLLVPSPGSFNTGELCEVRAAAASSHFTHLFIYLP
                     FASAIYMGAFNLSEENIGKGHK"
     gene            complement(3163..3984)
                     /locus_tag="PK733_01715"
     CDS             complement(3163..3984)
                     /locus_tag="PK733_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ09295.1"
                     /translation="MISKIIKLWDKDAYRSSEVTTFRPTLTTYILNGSRKRGAVLVVP
                     GGGYSFTSPREAEPIALQFNAAGYHAFVLDYSVAPAKHPQPLLDASRAMCIIRKNAEE
                     WNVDSEKIAVCGFSAGGHLAASLGVYWDKEYLSNIPGVEIELNRPNALILAYPVITSG
                     QYAHRGSFINLLGENATSELLYEMSLEHHVSEKTPPAFIWHTFADQGVPVENSLLFTQ
                     SLRNKSIPFELHIYPEGGHGLSLATEETATRPDQVVPHAATWIKLCLEWLNLQFS"
     gene            complement(4090..5250)
                     /locus_tag="PK733_01720"
     CDS             complement(4090..5250)
                     /locus_tag="PK733_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09296.1"
                     /translation="MRKIRFGIISCGMIADFHAKSILMDDRAELVAVYGHSNVEKTKA
                     FAKKYGVEKVYMDYKELAADKDIDAVCICSPSGLHGLHTIECLKNGKHVLCEKPLDIN
                     SEVMTDMIKTAEGKNLKLGCVFPNRTRSGLRKAKEIVDSGKLGRMTIVECQYRGYRSP
                     EYFTSSNWKGTKELDGGGCLMNQGIHAIDTMCWLTGDVKSVYGTAGAILRDIEVEDTA
                     MAILEFLNGAKGVLMGTTISNVPENAPEGDRIRIEFEKGSIVYAEGKAALYMRHNGVN
                     AGNVTNAGNAINADNAANTAGIANTINTAGESVDGEIIKIMLDDVNAEVVSSSSDPSN
                     VDMESHAFIVSDFVSAILENREPYIPGSSARKSVDLVLAIYKSSDIGEKVKL"
     gene            5598..6434
                     /locus_tag="PK733_01725"
     CDS             5598..6434
                     /locus_tag="PK733_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ09297.1"
                     /translation="MPDFKISVIVDSFRLGVREGIEKAKDVGASGIQLHATRGLMTPE
                     NLTVKQRKELLDIITSNGLFVSALCGDLGSGFAVKEENDWKIEKSKRIMDLAKDLNTN
                     VITTHIGVVPENSDHPRWGILQEACEALGEYGDSVGAAFAIETGPETSAVLRKFLDSL
                     SSKGVKVNLDPANLVMVTGDDPVKAVVTLKDYIVHTHAKDGIMLNKGNPEAIYGMIEN
                     KMTEPAFKEVPLGEGSVNFPEYLKALKSIGYSGFLTIEREVGDNPEKDIRAAVAFLNT
                     LI"
     gene            complement(6464..8797)
                     /locus_tag="PK733_01730"
     CDS             complement(6464..8797)
                     /locus_tag="PK733_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09298.1"
                     /translation="MSRNISGYIRKTMLLRLIVYFLLIIIITLFASSYFIYNYFSSSF
                     KSEINSYFDKVLNQVSIISDEFVLKKINELAIKQLIDTQKDSEIISIFNNSGIDDWKQ
                     VSDVQQKLDNIAFHYRDVLDTVFIHSRSNDILISSSRNALKYVNEESVTELDEYYWVS
                     SFYKSNKSIQWLKTRNAQVHLSSSSTRGDVITFICAYPISSTGDAVKGFIGVNIKEEA
                     LSNYLVKFNSSNTGMLMIIDNDGVIVSHSNKASLYEDISKEAFVKRILNSSHPVSFET
                     TFNNNKYVVSYVKSGFNNWYYVSLVTTELFYQKDQSIKKRIFLVSAVILFIVLILSNV
                     LSLKIYTPLRKILNKYSTSVAASNIPRKGIEEYNLLDNMFSNMHSKIDSLQESLSKNA
                     MLIKHNLMLDLLNNRLSRADSLENMLNLLNIKFLHKYYGAVIFSFNKEDIGLQQNNTQ
                     LYKYKIIDFIDILNNNNYTCIPVDIRNESICVILNTDSTRKDDIVQFINKVEDFCIDE
                     IGTRLITGIGRSVEDLYSINASYIDAKMCMQYNFIYPDKNMFYYDEFNVTENTIPEDI
                     SIKLDKALRLGSYTEVKKATDRFLAIATCDNIAYREVRREISRFISIFRQFTEDINLN
                     LNEVMNEYCKNRLYHPRNINVFINTFLQMVNSTNDIISRKKQNENLNIVNKIKQYVLD
                     NLHMDISLNSTAEVFSISPYHLSRIFKKETQVNYIDYVMTCKIDKAKNLLVTTDYSIE
                     KITELIGYNHSTYMSKKFKELTGKTPNEYRKDARMPI"
     gene            complement(9106..10773)
                     /locus_tag="PK733_01735"
     CDS             complement(9106..10773)
                     /locus_tag="PK733_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09299.1"
                     /translation="MRRKTLKMLALVITTVMTLTILSACSGTRKESTDKSASTTANTQ
                     TTIQENDPYAPKEDKVYEISYVTTGTSAVEAEKSKILKSYNDTFNVKLKPIFFEPAQF
                     EELLNLTLASNDIPDVWIAQNISSLWRYQEQGLLSEVKLDIVEKYAPDLFKCIDRDAK
                     DAWQTSKYKGKNYGIPLINASYMFRDAIAWRTDWLKNVGIDKIPETIEEWENALLKFR
                     NDDPDKNDKKDTYGCSTSIFAPVSGAFGTQYSMWWSGSWVEEDEQAVYTSVSPKMKDT
                     LALLNKWYKMEIIDPEFVTGENKGGYWAVSTDFVNGRIGVSAMGYYYHWYKEKKASNG
                     EVIVNPGSTAAEFTKNYPNGTFEFGNPPIGSDGKSRGMMSLGIVPGNTYVFSKNLEEE
                     PDKFGKILQMFNWPQESFENHLFAFMGIEGEQYTVRKIDNYTTYVQKLANEYTEEEKK
                     LYSGTGQFFLQNDIENYRKQYEILYDTAVKNGFNDYKLSHITNRLFIPSQGKLWADLN
                     KMEVDAYVNIITGKKPISYFDEFVANWKAAGGDQILKEANESFNNSK"
     gene            complement(10895..11773)
                     /locus_tag="PK733_01740"
     CDS             complement(10895..11773)
                     /locus_tag="PK733_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09300.1"
                     /translation="MVVRKNTLGENIFDVLNVTFLLIFCATCIYPFLYIFNLSVSEGL
                     SSSQYGLHLIPKGLTFEYYKKTLGSQIIYISFYNSVFRTVVATVLTVIFTVMPAYAIS
                     KKYFPDRGFWTMFILIPMFFGGGLIPTYFLIKSLNLINSRWVLILPGLISTYNIILIR
                     TFMQTIPDSLEESAKLDGANEPTILFKIILPICKPILATIALWTAVGHWNAWFDCILY
                     ITDEKKVVLQVVLQRIVTLGNAGLATNAASVEEQPPIFAVIACTIVIATLPIILVYPF
                     LQKYFVKGIMIGSLKG"
     gene            complement(11813..12817)
                     /locus_tag="PK733_01745"
     CDS             complement(11813..12817)
                     /locus_tag="PK733_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ09301.1"
                     /translation="MLANVIRSNNNSNNNSTGIVTHICTAISKKVHCYIKYKFLVLLF
                     LPAIVSFLIFNYAPMYGIIIAFKDFKFLKGILNSPWTSHNGFGHFYTMFSGRTFPKVF
                     LNTIYISSLKLIFGFPAPIILALLLNELRSIRLKKFIQTITYMNHFIGWVILTGILKQ
                     FLSPSIGPIGYIMQELGMQPINFLSDSHWFKAVLVSSSIWKEVGWGSVVYLATIAGIN
                     PELYEAAITDGATRLQCAIHITLPSLSFVIAFLFTMGLSSIINDDFDQIFNLMQNNPA
                     VLNSADVISTYTYRQGLKMMEYGYAAAVGLFKNAIAIIMLLVTDFVLKKSTQGEHGIF
                     "
     gene            complement(13133..14194)
                     /locus_tag="PK733_01750"
     CDS             complement(13133..14194)
                     /locus_tag="PK733_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09302.1"
                     /translation="MKKLRVGIIGTGKLAGLHVKAYCSNPDVEVVAVCDINGERAKQF
                     AAELGINTVYSDYRDMVASDQLDAVSIVTWNNTHAPIAIAALEAGKHVLCEKPPALNA
                     EEALKMLEASKRSNKLLMFGFIKRFARISQFMKEYIDSGELGEIYYAKAGYLRRCGNP
                     GGWFANKSLSGGGPIIDIGIHVMDLCMYLMGKPKPVAVFGNTYDRIGNRSNIKGYSWY
                     KATDYSRNLVGVEDSANALIKFDNGASLYIETSWTLNIAKDTVYSQIYGDKGGADIEP
                     DFNIYTEKNDYLVNCKPILDSYTFEFDECFAAQINHFVDCIINGSVCICPAEDGVTIM
                     KVIDAIYESARTGDIVKLN"
     gene            complement(14181..15497)
                     /locus_tag="PK733_01755"
     CDS             complement(14181..15497)
                     /locus_tag="PK733_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ09303.1"
                     /translation="MRYIKIGVAESDIELITGMDMTGYLARMFPSEGIHDALKIKCIV
                     FDDDKNQFVLIICDLLGLDSEYIKECNTEISKKLSISSDNIVIACIHTHSGPASIFLQ
                     DSGAVDYKWLESLKLRIVECACKAAYRMRSSKIIYKTSNCNIGINRVMWDVSNPELFV
                     DDQLGVLEIRDAGSGCIDTVLVNYACHPVVLGDANLFYSKDYPHFLIHALNRRDDYKN
                     ASIIFANGCCGDINPSEKGNFEIADKLGKRLADSVADTAIIQLDENVFSNAKIKVKTL
                     RVSIPLKNEAGDVLKEPKSENSDEVETQDNEIIIKVKKAMKNWGVRMRSKNSSGTLEY
                     AVAADIKIICIGMLRIVTLPFEVFHEIGIKIKEFFGTDRTIVLGYANGDYGYLPSKAL
                     YEKAGYEAGTAFKYYGFPGPVCKDAEDIVLKAIFDAAIEGGLIEKA"
     gene            complement(15567..16784)
                     /locus_tag="PK733_01760"
     CDS             complement(15567..16784)
                     /locus_tag="PK733_01760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ09304.1"
                     /translation="MSKLALKGGNKLRTEGFPMWPRYDGKELAALERVLNSGKWGTLG
                     GEVDRFAKSFAAYQHAKYGVAVTNGTVTLEVIMRALGIGYGDEVIIPPYTFNATSSAV
                     IFQGATPVFADIEEDTFNIDPEKIEKAITSRTKAIIPVHLGGRACNMDRIMEIAKKHN
                     LYVIEDSAHAQGSEWNGRRVGSIGHAGSFSFQASKNLTAGEGGFITTNDTDIYEKCWS
                     IHHCGRDIRGKVWYDHPIVGTNARMTEWQAAILNTQLERLDEQIEKRMSNAAYLNSRL
                     KEVPCVEILKEDKRITRNSHHIFIFRYKSEKCKGLDREVFLNALNAEGIPSSPGYVCL
                     YKQSLFNTPEFRRITGSNINYGELYLEKAEKICGKEGVWLTQNMLLAEKKDIDQIAEA
                     IIKIYENVDELVK"
     gene            complement(16818..17366)
                     /locus_tag="PK733_01765"
     CDS             complement(16818..17366)
                     /locus_tag="PK733_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ09305.1"
                     /translation="MKKVLLLGDSIRLNYQPQVGEKLNGIAEVVGPADNCRFTKYTLW
                     HIKIWVDELGKPDIIHWNNGLWDVYRHNTDVGIFTPLEEYLCELKRILTELRKTEARI
                     IWASTTPVNPNNEFTRNSDIDLYNKAAEEFMKKEQIEINDLNSQIKTNVQLFICQDYT
                     HLSEEGKEVCSDAVAAAIKKYI"
     gene            17810..18673
                     /locus_tag="PK733_01770"
     CDS             17810..18673
                     /locus_tag="PK733_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09306.1"
                     /translation="MKDASLYEKAGMYDNGFPIQLLKVEKNCQMMAFTSHWHEQLEIL
                     YFTEGNAVIQCNSISYEASPGDLIIINSNELHTGYNVGNYLAYYCFNIDSSFIHSRFM
                     DTCEMKYITPIERNMILFKNKVSGDNSIIECINAVIREYESKETAYELVIKSCAYKLL
                     AMLIRNHVTKVITHAEYAKSLCYLERLAKVFKYIEENYNQKITIEDLSSMSNLSSYYF
                     CRMFKMVTGKTMIEYINSFRINKADYLLKSTDMNVTEVAMATGFNDINYFSRTFKKYK
                     KISPSHVTKVN"
     gene            complement(18677..18838)
                     /locus_tag="PK733_01775"
     CDS             complement(18677..18838)
                     /locus_tag="PK733_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ09307.1"
                     /translation="MSDIIIKIIERRQELGMSQRDLENLTGIRQEAICRMETMKNVPQ
                     LDTLIKLLV"
     gene            19028..19885
                     /locus_tag="PK733_01780"
     CDS             19028..19885
                     /locus_tag="PK733_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOJ09308.1"
                     /translation="MIVTTTEVQNNFGKYLKLSEIEDVIISRNGKVVAKLTSCTSNKD
                     DFTIREAAREYIYSGKKVSYEEFLEISEKSELRYEYIDGEIYLLASPFYAHQKAIRQL
                     FVEFANWFKDKKCEPLSAPFDVTLFNVIGDIYTDMDTSAETATNIHTNNDTDAYADTE
                     TDTDTDTNTDTVDNKNKNINVVQPDILVICDKENINEKGRYTGTPSLVVEILSESTRS
                     KDLIKKLNLYMRSGVKEYWIVNTFSKEIYLYVFKDYNIDKMMAFKNGERAESDIFKGL
                     GVNLEHVFD"
     gene            complement(20072..20920)
                     /locus_tag="PK733_01785"
     CDS             complement(20072..20920)
                     /locus_tag="PK733_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09309.1"
                     /translation="MNGDTSSLGNGSEFDKNLDRNGVDIIMKKIDTNCLLGHWPFRKI
                     RMNKFDDLKTVHMQNGIEYGYLSSLDSIFYNDPFEGDIELHEIIKDTPYKHILTVNPE
                     VPGFIDDIKRGIKLFDIKGVRIYPCYHGYKLDNKYLRQLCHTLSENKLPLFLTMRMED
                     ERLNYLMQPKNIDILNLSDFLADHPENTVVLQSIRVGEVLALKDIINSRKNIFLDTSG
                     FKDWLFVIEKLIKEINHHRIVYGSNHPLYCLKSSLLLVEEAEVGNYVKEDIFFNNAGA
                     IPEFHS"
     gene            complement(20917..21669)
                     /locus_tag="PK733_01790"
     CDS             complement(20917..21669)
                     /locus_tag="PK733_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ09310.1"
                     /translation="MIIDIHAHIWGGNYENNKREIMKACELYNISKVYISGLRSYYSD
                     KDEINELNYEVHKFSKEQPDYIGGFCYVNPVNDNCIDVLKKGIEEYGMKGMKLWVATY
                     CDDPLVFPLVEKCIEYNIPVLIHSFKKAVGQLEFETTGPHTAELARRYPDARFIMAHL
                     GGNAYHGIKSIKNYKNVWVDISGSIFRRDDVDYTVEQIGAERILFGTDMTGSFLVNYG
                     QIEEANLTAEQRDLIYYKNAFKVLGCTEDGSL"
     gene            complement(21843..22007)
                     /locus_tag="PK733_01795"
     CDS             complement(21843..22007)
                     /locus_tag="PK733_01795"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HOJ09311.1"
                     /translation="MSETKELIVKIRKIKEELDRMIELKGNLQDYDIIKLSKELDELL
                     NEYDKIKRQV"
     gene            22310..22498
                     /locus_tag="PK733_01800"
     CDS             22310..22498
                     /locus_tag="PK733_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOJ09312.1"
                     /translation="MKAFVDKDACIGCGLCESLCPDIFKLEDEGTAVAIDEELTDENL
                     DCAKDAESQCPTEAIVVE"
     gene            complement(22588..23358)
                     /locus_tag="PK733_01805"
     CDS             complement(22588..23358)
                     /locus_tag="PK733_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOJ09313.1"
                     /translation="MIYTGTAGYSYKDWIGPFYPKGIKDGDMLEYYSRYFDFTEVNST
                     YYHMPSLRLFESLNRKTPNSFRFAVKLFGGFTHEKTAGTVEAEQFKYAIKPLLESKKL
                     ICLLVQFPYSFHFKPENMDYLKTLGEWFEGIGICIEFRNNNWIRKEVIDLMKNENLGF
                     VCVDEPRIKGLIGNTLAVTSKISYIRMHGRNAEKWYNSEGSERYNYLYSRDELLEWVT
                     RIQEVEGKSDITIVSFNNHPFGKAVENAKALREILFKN"
     gene            complement(23476..24612)
                     /locus_tag="PK733_01810"
     CDS             complement(23476..24612)
                     /locus_tag="PK733_01810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ09314.1"
                     /translation="MKINANANKTNVLSKFTAKIIIVLFIALSFAFIEYASNENHKIG
                     FGAFKKYGNMVDSNLKGNSAEVFYMINNYRKYYHLLPLIDNRYIKLQEESKAGEKLLN
                     EKDSKTDNNTDNIVDSITGNEADNKVGNVVGYETGDETACKTSAESSFKKDGQKVQSP
                     SKDEKGNQAQDKLSIDLKPVYNGNKSKNQVAITFDDGPDEHFTLRVLDILKGNNIKAT
                     FFVIGVNVKKYPKVLKRIREEGHDIGNHSWGHKNFAKLSEDKIKDEVLDTEELISSIL
                     EEHQPILRVPFGSSGDKADEVVNSLGYYNIRWSVDTQDWSGISSSEIMSRVKKQLYPG
                     GIILMHCSGKKEAMENSIKALPEVIKYIKDKGYEFVTISQMLEI"
     gene            complement(24915..25055)
                     /locus_tag="PK733_01815"
     CDS             complement(24915..25055)
                     /locus_tag="PK733_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09315.1"
                     /translation="MGALEIAIIVISAAALVGALVFNSYISKKAEESSKKQDNKGKIP
                     GK"
     gene            complement(25078..25461)
                     /locus_tag="PK733_01820"
     CDS             complement(25078..25461)
                     /locus_tag="PK733_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09316.1"
                     /translation="MRRYGWTYKLLAVFILVVILLQTSLLQRACIAGNRKYYYKSDIA
                     IINTEANHAVLSYADQEAWRKASRLLINRMYTTNFVKSCFLFYKNQPFKDKIILTDVR
                     KEISDFTTVYFFGSKYKSTFACCLL"
     gene            complement(25612..26382)
                     /locus_tag="PK733_01825"
     CDS             complement(25612..26382)
                     /locus_tag="PK733_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOJ09317.1"
                     /translation="MSIGSNYKLVITIIKKGMGTKVALSAKKAGADSETIILGRGTAD
                     KNVYLEILGIDYEPEKEIVFSVAKDENTDDVLKTITKESLLDKPGRGIAFVVEMGGLV
                     YMNEKVNERVNENASKNLKESPSGSVSENIIGASYAGKEGNIEFNLIVTIVNKGKAWS
                     VVEASKKAGAEGGTIMGGRGTGIHENLKILGIPIEPEKEIVLTLVPDNITEKVLDSIT
                     KDAELNKTGKGIAFILNVEKAVGICHALNKNLSGNKDR"
     gene            complement(26390..27121)
                     /locus_tag="PK733_01830"
     CDS             complement(26390..27121)
                     /locus_tag="PK733_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOJ09318.1"
                     /translation="MISSLFKGFSGIMLEVFYALLPILIIFIIFQIFVLRLSKHQLKR
                     IAAGVVLSFFGLSFFLQGVNIGFLPVGESMGIKLGEMKNNWILIPIGFLAGFSIAYAE
                     PTVRVLNDEVEKVSGGHINKTLMLYSLSLGVAISIALAMLRVLKGISLWYFLVPGYLL
                     AFFLLRHVDKTFVAIAFDSGGAATGPMTVTFILALTIGAAKGLGRGNPMLDGFGMVSL
                     VALASILSVLLLGYVYSKKEKNDKE"
     gene            complement(27118..27807)
                     /locus_tag="PK733_01835"
     CDS             complement(27118..27807)
                     /locus_tag="PK733_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOJ09319.1"
                     /translation="MNIKETVLEVVYAILPVTILVTIMQLTIVKLPKEDFVMFIGGTV
                     MVMLGLILFMIGVKAGFIPMGEMLGSALVSKGKLWLITIFGFILGFSVTVADPDVQVL
                     ASQVTRVSEGEIGRLLLIISVAIGVGIFISLALTRIFINLPMTYILIGGYTLAFIISL
                     FAPSRFFALSFDAGGVATGPMIVSFIVSLGVGVMSVTAKKDSSSDSFGLLGLACIGPI
                     LAVLILGVLVR"
     gene            28197..28640
                     /locus_tag="PK733_01840"
     CDS             28197..28640
                     /locus_tag="PK733_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOJ09320.1"
                     /translation="MPLSARELFARLLKCEADGEGIDGMKAVATVVMNRVHVAYGEYL
                     RECEGDLRRVIEQPYQFTCMLTQVYGQFNPQNIWATAPEQIHYDIADWALSGNKHQGS
                     NDCLWYMNPFSPTCPTIFPYNGTGSIFNRIGQHCFFIPTELYAQT"
     gene            28856..29302
                     /locus_tag="PK733_01845"
     CDS             28856..29302
                     /locus_tag="PK733_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09321.1"
                     /translation="MNSRNSCGNASPGMPVPYMQSTAISPGYEPKPFQPSGAVPIPMP
                     SLPTGIPTGPAYGGTVPTTPMPIPQPPAQAPQTVSSPYYLAGFLRNYVGRMVRVEFLM
                     GTTAPLVDRIGNLMEVGASYIVLRPVGTDDLIVADLYSIKFVTIYR"
     gene            29697..30866
                     /locus_tag="PK733_01850"
     CDS             29697..30866
                     /locus_tag="PK733_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ09322.1"
                     /translation="MSSLRKTLSLFLVLGLTTVFFAGCGGEKTADSSNEIKIGINYEL
                     SGKVASYGQSSVDGILMAFEEINAAGGVLNKTIKPVQADNKSDPAEATSIATRLATQD
                     KVVAILGPATSGAFKATIPVAIKNKVPVLSASTTDDAVTVDEKGVKEYAFKICFNDSF
                     QGTLMANFAIKNLNAKSAVILADNASDYAKGLAKNFRETFTNGGGKIVGEEGYVAGEQ
                     DFNAVLTKIKGLDFDVIFIPGYYEEVGLIIKQARDLEIDKPVLGADGFDSPDLVDLAG
                     KKALNKVYFSNHYSSLDKDPDVISFIENYKKKNGKEPNAFNALGYDLGYFIADAIKRA
                     DSTDPVKIKEALAATSNFKGVTGSFSIDDNHNAVKTAVVIELKDGEQASAVKVSP"
     gene            31025..31906
                     /locus_tag="PK733_01855"
     CDS             31025..31906
                     /locus_tag="PK733_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ09323.1"
                     /translation="MTQFLQQLINGLSLGSIYALIALGYTMVYGIIKLINFAHGDVYM
                     LGAYIGYACIKYFNLSFFPSLLIAMTACALMGFAIEKIAYKPLRNATRIAALITAIGV
                     SLFLEYSTMFVVKANVRTYPSVLNEISFKPLQGVIVNMKQVYIFAVTIILMIILQYIV
                     RKTRVGKSMRAVSTDRDAALLMGINVDRTISFTFAIGSALAGAAGVLVGIYYNSINPL
                     MGILPGLKAFIAAVFGGIGIIPGALVGGITIGVIETMVSGYGNSLYKDAAAFAILIII
                     LLIKPSGLLGKNTREKV"
     gene            31910..32884
                     /locus_tag="PK733_01860"
     CDS             31910..32884
                     /locus_tag="PK733_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ09324.1"
                     /translation="MAVNSIDMKSNPKMKWLKNIIVLVAFYVIMQVLISLNIINDYYQ
                     VTIAMICINIILAVSLNLITGFTGQFSLGHAGFMSIGAYTCALFTLNMSQQYSYFIGI
                     FAGAVMAAIIGALIGIPTLRLKGDYLAIATLGLSEIIRIIILNFEFTGGASGLSDIPQ
                     YTNWTWLFVFTAGTVIIINNFIRSSHGRACISIREDEIAAEASGINTTKYKVLAFVIG
                     AFFAGIAGALYSSYFYFVKPDIFGFLKSIDILVIVVLGGMGSISGSILAAVLLAIISA
                     YLQAFPDLRMVIYSILLVIIMIFRPQGLMGTKELSLNSFLKKRPAKVE"
     gene            32901..33686
                     /locus_tag="PK733_01865"
     CDS             32901..33686
                     /locus_tag="PK733_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09325.1"
                     /translation="MSILSVKNLTMTFGGLAALTNVALKLEKGELVGLIGPNGAGKTT
                     VFNLLTGVYVPTSGSIEYCAKDSMKKINGLSPHTISCMGIARTFQNIRLFKDLTVLDN
                     IKIAIHQNIKYGLVPAFFRTKSFHREEQEITSKAIDILEIFNLGVKKNELARNLPYGE
                     QRRLEIARALATRPSILLLDEPAAGMNPNETAELTKLIEWIRKEYELTILLIEHDMSL
                     VMKICERIYVLDYGMVIAEGKPEEIRSNKRVIDAYLGGEVLDA"
     gene            33730..34434
                     /locus_tag="PK733_01870"
     CDS             33730..34434
                     /locus_tag="PK733_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09326.1"
                     /translation="MLEINNIDVYYGVIHALKNVSLKVRKGEIVTLIGANGAGKTTTL
                     RTISGLIKPAKGSVFFNSEKISGISAQEIVKKGLSHVPEGRRIFPDMTVMENLELGAF
                     LRNDKIGIKSDLENIYSRFPILKKRKRQNAGTLSGGEQQMLAIGRALMSKPKLLLMDE
                     PSMGLAPLLVNEIFSIIKEINGLGTTILLVEQNANLALSIAHRAYVIETGSVVLEGSG
                     KELAKSEEIKKAYLGG"
     gene            34442..35083
                     /locus_tag="PK733_01875"
     CDS             34442..35083
                     /locus_tag="PK733_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOJ09327.1"
                     /translation="MNVKSRMTSNPYTISPEATIAEALELMRSNNVRRLPVVRNGKLV
                     GIVTEREIQEVSPSKATTLSIFELNYLLSKTKVSAVMTKEVITISPDALLEEAAVLMR
                     DNRVGALPVIQDGKVVGIITETNIFDAFIDLLGFRESGTRICVEVEDSPGILADVARV
                     IKGFGANITHLVVYRSNTGKSDIIIRVNTTNTDEMIQSLEKQGYKVTSVSKNY"
     gene            complement(35271..>35789)
                     /locus_tag="PK733_01880"
     CDS             complement(35271..>35789)
                     /locus_tag="PK733_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09328.1"
                     /translation="CCYDDTSSCAGRCCSDDTENSCFQKLAMHLKKIIKLGESYGIGV
                     LMENNSSVSFTHDIEFTGISKEFGLEGIQLVFNPLEYVRLKKHPFFHVFYNSKLKSSI
                     LFLRINDGLYVDGSPTFPGEGNAEVKEMASAMLARGFKGYFSFSPYFGSNMDIEQQAE
                     IIDKFKALLMKM"
BASE COUNT        11393 a         6849 c         5917 g        11630 t
ORIGIN      
        1 ccccggacaa atataaatac cttgaaattg tcgaaggtat agttgaaaaa agaggcctga
       61 aagttgacag agaatatttg caccgagagg ccctaaagtg ggaactatgg tataatggcc
      121 gctcacccag gactgcacgg cagtttgtgg actggctgga agggacattg taataataat
      181 tcgtgggtta catggagaca tgggttacat ggagacatgg agacatagag acagttcttc
      241 ataggtgggg acggttctgc ttagataaag tcagtacagc ttcaatgccg tactgatttc
      301 tattattaat aaaaaaatat cgcttttttg ttacaactac tataacaaac gtgtctaaat
      361 atatgaaacg gaaaggatcc ttatccgcgg gaggtgattt ggacatggga gatgatttaa
      421 acctggttaa aaaagtatta aaaggtgaaa cacaatattt ttcaaatctt gtagagaaat
      481 acgaaaatag ggtgtacaag acctgttaca ggtttgtccg gaatgaggaa gacgctcttg
      541 accttgcaca agaggtattt ataaaggtat ataacaatct tgcaggtttt aaacattcca
      601 gttctctatc aacatggata tatagaataa gtgtaaatac ctgtctcaac tatttaagaa
      661 aaaacaaccg gcttttattg aatttcgagg atttcaacaa ttcattgaac tacagggatt
      721 acaagggtat ctctgaagat tccggcggtt gtgattatgt gagttcagat ggcaatatca
      781 gtccagagac ccatatcggc tcagatactt tgggaagcac atcaggcaac ccggaaaact
      841 acgttcaaat gaaagagatg cttgaattgc tggaccttaa cctggaaagg gcaggaaaca
      901 aaagccggaa aatcttcatg tacagactat ttcatggaat gccttttaag gaaattggga
      961 aaaagatcgg agtgactccg gaagcagcca gaatgaacta ttcaagaact acaagatctt
     1021 taagaaagag ttttagggag tatcaaaaag gagagtgatt ataatgtttt gcaattcaat
     1081 tgaggagtat tcattactat acctggaaaa tgaagtcagt acaaaagaaa agaagctttt
     1141 tataaaacat ataaataagt gtgacagatg taaaaggatg tataacctta tcaggagcaa
     1201 ctatcatcct gaaacgaaag tagcagttaa gagccctgtg gtctcagaca aaattaacat
     1261 tcttatagac aggggtcgtt atcctgatac gaaaacagtt aataaagtat tttcaaagaa
     1321 gcggattgta ctggtatctg taattgttat actgtctgtg tttgctacag ttaacgcaaa
     1381 aactatatat aaaacatata aagaacttgt aaattcaatg tttgttgaca gatctctcct
     1441 gaatatgtca ggaataatac acacaggaat gtatagcgat aaagattatg ttaaaagtgt
     1501 atatgtgaaa taccaggata gaattgatga atatataact ggaatgaaaa cagcagaaat
     1561 atccaagctt gaatatgatg aagatggtga aaacttcaaa gtgcagattg ctaaaggtat
     1621 taaaggaaac tttatttcat atggttttga agggtataca atatttcata atgaattttt
     1681 tgataaatat acaatggggc ttgcacaggg catgcagagt atatttaata atagagagga
     1741 atattattct atcgatgagc tgctcaatgc tgcaggaatt tgccctatac taggatatgt
     1801 ccctgatgga tataatttta aaagttcgta tcttgtatcc aggaatcaag gaggaaatat
     1861 acctgatgaa ttgggattac gttaccaaaa agcagaagat gaatccttat atattgctct
     1921 tacacgcaga agaaaagcaa tagataatgt tgatgtcgca aacgaagaaa aacatgctgc
     1981 gacaaatgag gaattagata ttaatggata tcaggctgta tatattgaag aagatgttga
     2041 aagctcaaaa aaggacgaag tatataagac tttcaggagt gtcagcgtgt ttttaggaga
     2101 tcaggctaaa ttaccaatac tactagtaga atcagcatcc ttagggagag aggaattgat
     2161 aaatgttgcg ggaaatatta cggttaacaa tatttctaaa aaccagataa aagctgaaga
     2221 gtactttaga ggtataaaag acgaaaatat tcttgcttat gctgataaat atctggataa
     2281 catcaaagcc ggaaaaactg aattcaaatt caagatagat aaaaatactg aattctacaa
     2341 aagtggaaac aatgatacct attatataat gtatcgcaat gctgacatta acaagattag
     2401 ctctttcatt cagcttccaa ttaaattaaa cgatgatttt ctaaacagat ttcagcgctc
     2461 tgatgttcaa gtcagcgggc aaccttacaa tgaacaaaag tcagtttatt tgaatttaaa
     2521 aaatgaaaac aataatacat tcagcttgtt ttcagataaa atgagaagga ttggggagga
     2581 attaggctta actgaccaac tgatagcgaa aatgagcggc atacaatatg aagcagagcc
     2641 cctgttcagc agttcaggac atttatatta cctgatagag tctactaaaa aaacctggat
     2701 tcttcaaact ttatacgaag agaatgatct ggtaggaagt tacaacttta gtatagataa
     2761 aaaaatacta tctgataggg aagctataat aaagtttatc aatagccttt aacaaaatat
     2821 accattggat gcattggata tattggagat attggagata ttgggaatgg ttctcctggt
     2881 tccttctccc ggatccttta atacagggga attatgtgaa gtgagggctg ctgctgcaag
     2941 ctctcacttc acacatctat ttatatatct accatttgca tctgccattt acatgggtgc
     3001 atttaattta tctgaagaaa atattggtaa ggggcataag tgaaggctct aagcaccgta
     3061 acgatagccc ctggtaaata tttcttcaga tttgataaat gcgccccatt tacatctgtt
     3121 atttgcatct catctgccat tcgcatccgg catttgcacc cgttaactaa actgcaaatt
     3181 cagccattcc aagcaaagtt taatccatgt agcagcatgg ggaactacct gatccggtct
     3241 ggtcgcagtt tcttctgttg caagtgataa gccgtgtcca ccttccggat aaatatgaag
     3301 ctcaaaaggt atacttttat tccttaaact ctgagtaaaa agtaatgaat tttcaacagg
     3361 aactccctga tctgcaaagg tatgccatat aaaggcaggc ggagtttttt cactgacatg
     3421 atgttcaaga gacatctcgt aaagaagctc tgatgtagca ttttcaccca gtaaattaat
     3481 aaaagatcct ctatgcgcat actgccctga agtgattaca ggataagcca atatcagtgc
     3541 attcggacga ttcaattcaa tttctacacc aggtatattg cttaggtact ctttgtccca
     3601 gtacactcca aggctcgcag caagatgccc gcccgctgaa aacccgcata ctgcaatttt
     3661 ttcagagtct acattccatt cctcggcatt tttacgaata atgcacatag cacgcgaagc
     3721 atccaataaa ggttgaggat gctttgccgg agctacgctg taatcaagca caaatgcatg
     3781 ataccctgct gcattaaatt gcaatgcaat aggttccgcc tctcttggag aggtaaacga
     3841 atagcctcca cccggcacaa ccaggacagc tccccttttt cttgaaccat taagtatgta
     3901 agttgttaaa gtaggcctga atgtggttac ctcgctgctt ctataagcat ccttatccca
     3961 taatttaata atttttgata tcataatttt tgccccctgt tattgataaa taactgcttt
     4021 agttgctgat tgtctgccat ataaatctat ataaaactat actgtttacc aaaatcatct
     4081 acaacattgt catagcttaa ccttttcccc tatatcagac gatttatata tagccagaac
     4141 cagatcaacc gattttcttg ctgaactgcc cggtatataa ggttctctgt tttcaagaat
     4201 agcagaaaca aaatccgata caataaaagc atggctttcc atgtcaacat ttgaaggatc
     4261 tgatgaggaa gatacaactt ctgcatttac atcgtccagc attattttta taatctcacc
     4321 atctacagac tctcctgcag tattaatggt attagcaata ccagctgtat tcgcggcatt
     4381 atctgcattg atggcattgc ctgcattggt tacattgcct gcattgactc cattatgcct
     4441 catatacaaa gctgcttttc cttctgcata tactattgag cctttctcga attcaattct
     4501 tatcctgtcg ccttcaggag cattttcagg aacattggaa atagttgttc ccatgaggac
     4561 tccctttgca ccatttaaaa actccagtat tgccatggca gtgtcctcaa cctcaatatc
     4621 tcttaatata gcccctgcag ttccatatac gctctttacg tcacctgtca accagcacat
     4681 agtatcaatg gcatgtatcc cttgattcat aaggcagccg cctccatcaa gctcttttgt
     4741 ccctttccag ttggaactgg taaaatactc aggagaccta taccctctgt actgacactc
     4801 aacaatagtc atcctgccaa gcttgccact gtcaacaatc tccttcgcct tcctgagacc
     4861 gctccttgtt ctattaggga acacacatcc cagcttaaga ttttttccct cagcagtttt
     4921 aatcatatct gtcataactt cactattaat gtcaaggggc ttttcacata atacatgctt
     4981 tccattcttc aagcactcaa ttgtatgtag tccatgcaga ccgcttggcg agcaaataca
     5041 tactgcatca atatccttat cagcagccag ttccttgtag tccatataaa ccttttctac
     5101 tccgtacttt tttgcgaaag cctttgtctt ttcaacattg ctatgaccgt atacagccac
     5161 aagttcagct ctgtcatcca taagaattga tttagcatga aaatcagcta tcatcccaca
     5221 tgatattatt ccaaagcgga tttttctcaa tttaaacact cccatctaat cggtttatct
     5281 gcatcatgta cagtatagca aaacttatgt tgtatgacaa tcactttgtg aagggcacat
     5341 tttattctgg aactattata attgagattt cagtataaga aacatatcca ttcacatcct
     5401 tttgagaagt gccttgtaga atggatgaag aaggaatata taaattatgg aaaataatta
     5461 tatatataca gcaaaataat gcttgttttc cacaaatata tgcatgaaaa aaaaactgta
     5521 ttcatgtaca attataaaca gcacaccgct ttctttactg ttgtgtgcta aatactatgc
     5581 attggaaggt gaattaaatg cctgatttta aaattagtgt tattgttgat tccttcaggc
     5641 ttggtgtcag ggaaggaatt gaaaaagcaa aggatgtcgg tgcttcagga atacagcttc
     5701 atgctaccag aggtctaatg actcctgaaa atcttaccgt gaaacagaga aaggaactcc
     5761 tcgatattat cacatcaaac ggactttttg tttccgctct ttgtggcgat ctgggaagtg
     5821 gatttgcagt caaggaggaa aatgactgga aaatagaaaa atccaagaga attatggatc
     5881 tggcaaagga cttgaatacg aatgtcatta caacacatat aggagttgtt cctgaaaatt
     5941 cggaccatcc gaggtggggc atactgcagg aggcttgtga ggcacttgga gagtatggcg
     6001 acagcgtagg tgcagctttt gcaatagaga cgggacctga aacctcggct gtattaagaa
     6061 agtttcttga cagcttgagt tcaaaaggtg tcaaggtcaa cctggatcct gcgaatctag
     6121 ttatggtaac cggtgatgac cctgttaagg ccgtggtgac acttaaggat tatatagtgc
     6181 atacacatgc aaaagatgga ataatgctta ataagggaaa tcccgaagct atctacggta
     6241 tgatagaaaa taagatgaca gagcctgctt ttaaggaagt tccgcttggc gaaggcagtg
     6301 tgaacttccc ggaatacctc aaagcattaa aatcaatagg atatagcgga tttttaacaa
     6361 ttgaaagaga agtaggagat aatccggaga aagatatcag agcagctgta gcattcttga
     6421 atacactcat ttgataaggt gttatttatt ttgctctata gcactatatt ggcatacgtg
     6481 catcttttct atattcatta ggtgttttac cggtaagctc cttaaatttt tttgacatgt
     6541 atgtggaatg gttgtaacca ataagctctg taattttttc tatactgtaa tcagttgtta
     6601 caagcaggtt ctttgcttta tcgattttgc aggtcatcac ataatcaata tagttgactt
     6661 gtgtttcctt cttgaaaatt ctgcttagat gatacgggct gatactaaaa acttctgcag
     6721 tagagtttag cgaaatatcc atatgcagat tatcaagaac atactgcttg attttattaa
     6781 caatatttaa attttcattt tgctttttcc ttgaaataat gtcattggtt gaatttacca
     6841 tttgcaaaaa agtattaata aacacattaa tattgcgagg atggtacaat ctatttttac
     6901 aatattcatt catgacctca ttaagattta agttaatatc ttctgtaaac tgcctgaata
     6961 tagatataaa acgagaaatt tcacgcctga cctcacggta ggcaatatta tcacaggttg
     7021 ctatagctag aaatctgtca gttgcttttt ttacttctgt atagctgcca agcctcagag
     7081 ctttatcaag ctttattgag atatcctcgg gtatagtgtt ttcagttaca ttaaactcat
     7141 cataataaaa catgttttta tcaggataaa taaaattgta ttgcatgcac atttttgcat
     7201 caatatatga agcgtttatt gaatataaat cttctacgga cctccctatg ccggtaatca
     7261 gacgggtccc gatttcgtca atacaaaagt cctctacctt atttataaat tgtacaatgt
     7321 catctttccg agttgaatca gtattcagta tgacgcatat tgattcattt ctaatatcta
     7381 ccggtatgca tgtataatta ttattattaa gaatatctat aaaatcgatt atcttatatt
     7441 tatagagttg agtattattc tgctgcaatc ctatgtcctc tttattgaat gaaaaaatta
     7501 cagcaccgta gtatttgtgc aaaaatttta tatttaaaag gttcaacata ttttcaaggc
     7561 tgtctgcacg agaaagacgg ttattgagca aatcaagcat aagattgtgt tttatcagca
     7621 tggcattctt actgagagat tcctgtaaac tatctatttt tgaatgcata ttgctaaaca
     7681 tattatctag cagattatac tcctctattc ccttcctggg tatgttggat gctgcaacgg
     7741 aagtagaata cttgttaagt attttcctga ggggagtata tatttttaaa gataagacat
     7801 ttgacaatat aagcacaata aagagaataa cagctgaaac aagaaatatt ctttttttaa
     7861 tagattgatc cttttggtaa aatagctcag tcgtaacaag cgaaacataa taccaattgt
     7921 taaagccaga ttttacatag gaaacaacat acttgttatt attaaatgtt gtttcaaagc
     7981 taacaggatg tgacgaattt aaaatccttt ttacgaatgc ctcctttgaa atatcttcat
     8041 acaaactagc cttattacta tgcgatacaa ttacgccgtc attatcaata atcataagca
     8101 ttcccgtatt acttgagttg aatttcacca gataattact taacgcctct tctttaatat
     8161 tgactccaat aaagcctttt actgcatctc ctgtagaaga tatggggtac gcacagataa
     8221 aagttattac atctcccctg gtagaggaac tacttaagtg cacttgagca tttcgcgttt
     8281 ttagccattg tatgcttttg tttgatttat aaaagctact tacccaatag tattcatcta
     8341 attcagtaac tgactcttca tttacatatt ttaacgcatt cctgcttgaa gaaatcagaa
     8401 tatcattact ccttgaatgt atgaaaactg tgtctaatac atccctgtaa tggaatgcta
     8461 tattgtccag tttttgttgt acgtcagata cctgtttcca atcatcaata ccgctattgt
     8521 taaaaataga tattatttcc gaatctttct gggtatcaat tagctgtttt attgctagtt
     8581 catttatctt tttcagcaca aattcatcag atattatgct cacctgattt aataccttat
     8641 caaaatagct attgatttcg cttttaaacg aagaggaaaa atagttatat ataaaatagc
     8701 ttgaggcaaa aagagttatt attataatca aaagaaagta tactatcaac cttaaaagca
     8761 ttgtttttcg tatatatccg gatatatttc tgctcatgct aacagccacc ttatcaagta
     8821 ttaatatgct ttacaaaatt cttatattta atctttctta tgtctcagtt tcttatgtcc
     8881 cagtttcttg aacacaaata attataacat ttttagctct ctttttgtat atatttcttt
     8941 tgccttttat tttctgttcg cctccagccc taaggcattc aaatgataaa aagtcgtcaa
     9001 atacttcaaa ctacagatac ccaaaatctg cgttcaaagt ccttgcgcag attttgggta
     9061 ctaccggttt aaatatactt tttaaactaa ctcaggttat aaatgctatt ttgaattatt
     9121 aaaagattcg ttagcttcct ttaaaatttg atcaccaccg gctgccttcc aatttgctac
     9181 aaactcgtca aaataggaga tcggcttctt tccagttatt atattgacat aagcatctac
     9241 ttccatcttg ttaagatcag cccataattt cccttgggac ggtataaaca gtctgtttgt
     9301 tatatgtgat aacttataat cgttaaagcc atttttaact gcagtatcat ataatatctc
     9361 atattgcttt ctgtagtttt ctatatcatt ctgtaaaaag aattgaccag taccgctata
     9421 tagcttcttt tcctcttcag tatactcatt tgccaatttt tgtacatatg ttgtatagtt
     9481 atcaatcttc cttactgtat attgttcacc ttctataccc ataaaagcaa ataaatgatt
     9541 ttcaaaactt tcctgaggcc aattaaacat ttgcagtatc ttgccaaact tatccggttc
     9601 ttcttcaaga ttttttgaaa atacatatgt gttaccagga actataccca atgacatcat
     9661 tccccttgat tttccatccg aaccgattgg cggatttcca aattcgaaag tgccgttggg
     9721 ataattcttt gtaaactctg ctgctgttga accaggattt actattactt ctccattgga
     9781 ggcttttttc tctttatacc aatgataata ataacccatg gcagagacac ctattcttcc
     9841 atttacgaag tctgtcgaca cagcccagta accgcccttg ttttcaccag ttacaaattc
     9901 cgggtcgatt atctccattt tgtaccattt gttaaggaga gccagagtat ccttcatttt
     9961 tgggctgact gaagtataaa ccgcctgttc gtcttcctct acccaagatc cactccacca
    10021 cattgaatac tgagttccga aagcaccgct aactggtgca aatatagatg tagagcatcc
    10081 gtatgtatcc ttcttatcgt tcttatcagg atcatcgttt ctgaatttca acagtgcatt
    10141 ttcccattcc tctattgttt ccggaatttt atctattcca acatttttta gccagtccgt
    10201 tctccatgca attgcatctc tgaacatata gcttgcattg ataagaggaa ttccataatt
    10261 tttgcctttg tattttgaag tttgccaggc atcctttgca tctctgtcta tacatttaaa
    10321 cagatcaggt gcatactttt caacaatatc cagttttacc tctgaaagca aaccctgttc
    10381 ctgatatctc caaagtgaac tgatattctg agctatccac acatcaggta tatcatttga
    10441 agccagtgtt agatttaaga gttcttcaaa ttgggcaggt tcaaaaaata ttggttttag
    10501 ctttacatta aaagtatcat tataggactt taaaatttta cttttttctg cttctactgc
    10561 ggatgtacct gtagtaacat agctgatttc ataaaccttg tcttccttcg gggcatacgg
    10621 atcattttct tgtatagtcg tctgggtgtt tgctgttgta cttgcactct tatccgtact
    10681 ttcctttctc gtaccgctac atgcgcttag aattgtcagt gtcataactg tggtaatcac
    10741 caacgcaagc atttttaatg ttttccttct cattttgttt acctccattt ttatttaata
    10801 tactctatag ataacacatt aatcaaatca catcgtgatt cagattatgc atcattctat
    10861 ccctgatttt aatatccctg attttgatat gcactcatcc ttttaaagac ccaatcataa
    10921 tacctttaac aaagtatttt tgcaggaaag ggtataccag aattatcggc aatgtggcta
    10981 ttacaatggt gcacgcaata acagcaaata tcggcggctg ttcttctaca gacgcagcgt
    11041 tagttgccag acccgcattt cctaatgtga caatacgctg aagcaccacc tgcagcacga
    11101 cctttttttc atcagtaatg taaagaatgc aatcaaacca tgcattccaa tgtcctactg
    11161 cagtccagag agctatcgta gccagtatcg gcttgcatat aggcaatatt attttgaaga
    11221 gtatagttgg ttcatttgct ccgtccaact ttgcagattc ctcaagacta tcaggaattg
    11281 tctgcataaa tgttctgata agaataatat tgtaagtgct tataagaccc ggcaaaatca
    11341 atacccatct gctgttaatc aggttaaggg attttataag gaaatatgta ggtatcagcc
    11401 cgcctccaaa aaacattggt atcaatataa acatagtcca gaagcctcta tcaggaaagt
    11461 atttcttgga aatagcataa gcaggcataa cagtaaatat aactgttaag accgtagcca
    11521 caactgttct aaaaaccgaa ttataaaaac tgatatatat tatttgtgaa ccaagtgtct
    11581 tcttatagta ttcaaaggtt aatcctttag gtataagatg aagaccatat tgtgaacttg
    11641 aaagtccttc acttacagag agattaaata tatagaggaa tggataaatg catgttgcac
    11701 aaaatattaa aagaaaagta acatttaata catcaaaaat attttcacct aacgtattct
    11761 ttctaacaac caagcttctc actcctttga tcaaaagcta tacttgcttt tactaaaata
    11821 taccatgttc accttgagtt gattttttca atacaaaatc cgtcaccaag agcattatta
    11881 ttgcgatagc atttttgaac agccctactg ctgctgcgta gccgtattcc atcatcttta
    11941 gcccctgcct gtatgtgtat gtactgataa catcggcact attcaacact gccggattat
    12001 tttgcattaa attaaatatc tggtcaaaat catcatttat aatactactt aatcccatcg
    12061 taaaaaggaa tgcaatgaca aagctaagtg acgggagcgt aatatgaatt gcacactgca
    12121 aacgtgttgc gccatccgtt atggctgctt catacaattc tggatttata ccagctattg
    12181 ttgccagata aactacgctt ccccatccta cttctttcca aatggatgat gaaaccagga
    12241 cagctttaaa ccaatgagaa tcagaaagaa aattaatggg ctgcataccg agctcctgca
    12301 taatgtatcc tataggacct atagaaggtg aaagaaattg ctttaatatg ccggtaagta
    12361 ttacccagcc aataaaatgg ttcatatagg taattgtctg gataaacttt tttaaccgaa
    12421 tactccgtag ttcattcaaa agtaaagcaa gtataatcgg tgcaggaaac ccaaatatca
    12481 gttttaatga acttatatat attgtattta agaatacttt tggaaaggta cggccactga
    12541 acattgtgta gaaatgaccg aaaccattat gagaagtcca gggactattt aaaattcctt
    12601 ttaaaaattt aaaatcctta aaagcaataa ttataccata catcggagca taattaaaaa
    12661 taagaaaaga gactatagcc ggtaaaaaca acagaacaag aaacttatat ttaatataac
    12721 aatgcacttt tttagatata gcagtacaaa tatgtgttac tataccagtg ctattgttat
    12781 tgctattgtt attgcttcta ataacgtttg ctaacattaa gaatcctcct tcattgctgt
    12841 aatattatta taatcgaaat tttcctaaat ttagcaaata cctattttac acttaagcat
    12901 ttaaaatcta gaaacagtaa tcaagcttga aaatttttta gtacatatta gtgccggtca
    12961 atataattat attaagtctc tcttaataag caagtactca ttcttgtgta ttctatgttt
    13021 tcttgctttt tttcaatact ttcttctact ctttcttgct atttgtatgt tttattgctt
    13081 tttcaatttt tttacattct aaatttctct tcagatacat aacactcata ttttaattaa
    13141 gctttactat atcacccgtt cttgctgatt catatatagc atcaatcact ttcattatag
    13201 taacaccatc ttccgccggg cagatacaga cactaccatt tataatacaa tctacgaaat
    13261 gatttatctg tgcggcaaag cactcatcaa attcaaaagt ataactatcc aatatcggtt
    13321 tacaatttac caggtagtca ttcttttcag tatagatatt aaagtcaggt tcaatatcag
    13381 ctccaccttt atccccataa atctgcgaat aaacagtatc cttggctata ttcaatgtcc
    13441 agctggtttc tatatacaaa gatgcaccat tatcaaactt tattaaagca ttggcagagt
    13501 cctctactcc aactaaattt ctactatagt cagtcgcttt ataccaggaa tatcccttaa
    13561 tatttgacct gttgccaatc ctgtcatatg tgtttccaaa aacagccaca ggcttgggct
    13621 tacccattaa atacatacac aagtccatga catgtatgcc aatgtctata ataggcccgc
    13681 cgcccgacaa ggacttatta gcaaaccagc ctcccggatt tccgcacctc ctcagatagc
    13741 ctgctttagc atagtaaatc tctccaagct ccccactatc tatatattcc ttcataaact
    13801 gcgaaatcct ggcaaatctc tttatgaacc cgaacataag cagtttattg cttctttttg
    13861 aggcctcgag catcttcaga gcttcttcgg cattaagagc cgggggcttc tcacaaagta
    13921 catgcttgcc tgcttccagt gctgcaatag caatcggggc atgagtatta ttccaggtga
    13981 caatgcttac agcatcaagc tgatcagatg caaccatatc cctgtaatca ctataaacag
    14041 tatttattcc caactcagct gcaaactgct ttgcccgttc tccattaata tcgcatacag
    14101 caacgacttc tacatccgga ttgctgcaat aagcttttac atgcaagcct gcaagcttac
    14161 ctgttcctat aatacctact ctaagctttt tcaattaaac caccctctat tgcagcatca
    14221 aatattgctt ttaatactat gtcttccgca tctttgcaga ccggccccgg aaagccgtaa
    14281 tatttaaacg ctgtacctgc ttcatatccg gccttttcat aaagagcctt tgaaggaaga
    14341 tagccatagt ctccatttgc gtatcccaat acaattgttc tgtcagtacc aaaaaattct
    14401 tttattttaa tgcctatttc atgaaacacc tcaaaaggca aggtaactat tctaagcatg
    14461 ccgatacata ttatttttat atctgctgca actgcgtatt ccaaagttcc gctgctattc
    14521 ttgctacgca ttctgacacc ccaatttttc atggcttttt tcactttaat aataatttca
    14581 ttatcctgag tttctacttc gtctgagttc tcacttttcg gttcctttaa tacatcaccg
    14641 gcttcatttt ttaaaggaat actgactctt aatgttttta cttttatttt agcattggaa
    14701 aatacatttt catcaagctg tataatcgca gtatcagcta cactgtcagc caatcttttg
    14761 ccaagcttat cagcaatctc gaaattacct ttttcagatg ggtttatatc accgcagcat
    14821 ccatttgcaa atattataga agcattttta taatcgtccc gtctgttcaa cgcatgtatc
    14881 agaaaatgcg gatagtcctt gctgtaaaac agatttgcat ctcctaatac tacaggatgg
    14941 caggcataat taacaagaac tgtgtcaatg catccacttc ctgcatccct tatctcaagt
    15001 actccgagct gatcatctac aaaaagctcc gggttgctaa catcccacat aacacggttg
    15061 atacctatat tacaattgga ggttttatat atgatttttg aagacctcat tctataagca
    15121 gctttacatg cacattccac aatacggagc ttcaaggatt ccagccactt ataatctacc
    15181 gctccgctat cttgcaaaaa aatcgacgcc ggaccggaat gagtgtggat acatgctata
    15241 acaatattat cagaagagat ggacagcttt ttactgattt cagtattgca ctcctttata
    15301 tattcactgt ccaatccaag gaggtcacat attattagca cgaattggtt tttatcatca
    15361 tcaaatacaa tgcatttaat ttttaaggca tcatgaatac cctctgaagg gaacattcgt
    15421 gcaagatatc ctgtcatatc cattcctgtt attaattcaa tatctgactc agcaacacct
    15481 atttttatat atctcatggt aatcttcctt tcctatactc cagtatgccc atatcaaatc
    15541 cgatttgcaa ttatcttata tctgtgttat tttaccagtt catctacatt ttcatatatc
    15601 tttataatag cttcggcaat ttgatcaata tcttttttct cagcaagcag catattctgt
    15661 gtaagccata cgccttcttt tccacatatt ttttcagctt tctctagata caattctccg
    15721 tagtttatat ttgaacctgt aattctcctg aattccggcg tattgaaaag agactgctta
    15781 tataagcata cataccctgg tgaacttgga ataccttcag cattcagcgc attcaggaaa
    15841 acttccctgt ccaggccctt gcacttttca cttttatatc tgaagataaa tatatgatgt
    15901 gaatttcttg taattctttt atcttctttc aatatttcga cacagggaac ctccttcaat
    15961 cgtgaattca aataggccgc gttactcatt cttttttcaa tctgttcatc aagccgctcc
    16021 aattgtgtat taagaatcgc cgcctgccat tctgtcattc tggcattggt ccctacaatt
    16081 ggatggtcat accatacttt accacgaata tctcttccgc agtgatgaat agaccagcat
    16141 ttctcataaa tatctgtatc atttgtcgta ataaagccgc cttcacctgc tgtaagattt
    16201 ttacttgcct gaaaactgaa agaccctgca tgtccgatag agccgactct tctgccattc
    16261 cattcggaac cttgtgcatg agctgaatct tctattacat ataaattatg tttttttgca
    16321 atttccataa ttctatccat gttgcaggct cttcctccca gatggacagg tattatggct
    16381 tttgttcttg aagttattgc tttctcaatc ttttccgggt caatattgaa agtatcctct
    16441 tctatgtccg caaaaacagg tgtagccccc tgaaatatta ctgctgatga agtagcattg
    16501 aaagtatagg gaggaataat aacttcgtct ccatatccaa tccccaaagc ccgcataata
    16561 acttccagtg tgactgttcc atttgttact gccacaccat attttgcatg ctgatatgct
    16621 gcaaaagatt ttgcaaatct atccacttca ccgcccaatg ttccccattt gccgctattt
    16681 aagactcttt caagagcagc taattctttt ccatcatacc tgggccacat gggaaacccc
    16741 tcagttctta acttattacc accttttaaa gctaacttac tcataacaat atcctcctaa
    16801 aaatttaatt gtactgtcta aatatatttt tttattgctg ctgctactgc atctgagcaa
    16861 acttccttgc cttcttcgct taaatgagta taatcctggc aaataaacag ctgcacattc
    16921 gttttaatct gactgtttaa atcattaatc tcaatttgct ccttcttcat gaattcttca
    16981 gcagctttat tatacagatc aatatctgaa ttccgggtga attcattatt gggattcacc
    17041 ggggtagtag aggcccatat aatccgagct tctgtttttc ttaattcggt tagaatcctt
    17101 ttaagctcac acaaatactc ctccagcgga gtaaatattc ccacatcggt attatgccga
    17161 tagacatccc acagaccatt gttccagtgt attatatccg gctttcccaa ttcgtccacc
    17221 catattttta tatgccatag tgtatatttc gtaaacctgc aattatccgc aggtcctaca
    17281 acctcagcaa taccattaag tttttctcca acctgcggct gataatttaa tctgattgaa
    17341 tctcccaaca gtaatacctt tttcatatgc tctttctcct gtaaattctt taaactgcca
    17401 agaaaattcg cttcgctcat gactcaaaac aatgatgcga atttacttgt tcgctaggga
    17461 accctagttt cccttcgacg gattctgttc tcttgcttgc tgcagaatcc tgagcaccct
    17521 ccttaaagcg gaaaggggat tccccttttc aaaccccagt ataggaattt tttaagagtt
    17581 ttataaattc ctatactcta aaaaatgcct acgctttggc attagaaact tgctgcccga
    17641 cagcgtatga tttaattata taaatacttg caaagtattt catgaataaa gatgctatct
    17701 tttagcataa ttttgtcttt ccccaatatt tttttaattt ttatatataa aaaaaagtta
    17761 taatgtttta taatagtact ggacttttag atgcaagggg gattaataca tgaaggatgc
    17821 ttcattatat gaaaaagcag gtatgtatga caacgggttt cctatccaat tattaaaagt
    17881 agaaaaaaat tgccagatga tggctttcac aagccattgg catgagcagc ttgaaatact
    17941 gtattttact gaaggaaacg cagttataca gtgtaattct atctcgtatg aagcaagtcc
    18001 cggtgacctt attattatta acagcaatga acttcataca ggctacaacg tcggtaatta
    18061 cttagcatat tattgtttta acatagattc ctcattcata cacagccgct ttatggacac
    18121 ctgtgaaatg aaatatatta caccaattga acgcaatatg atcttgttta aaaataaagt
    18181 aagcggcgac aactcaatta ttgagtgtat taatgctgta atccgtgaat atgagagcaa
    18241 ggaaacagcc tacgaattgg taataaaatc ttgtgcatat aagctgttag caatgcttat
    18301 aagaaatcat gtaacaaaag tcattactca tgcggaatat gcaaaaagtt tatgttatct
    18361 ggaaagactg gcaaaagtct tcaaatatat tgaagagaat tataatcaaa aaattaccat
    18421 agaggattta tcttctatgt caaacttaag cagctattac ttctgtcgta tgtttaaaat
    18481 ggttactggt aaaaccatga ttgaatatat taattccttt cgtatcaaca aagctgatta
    18541 cctattgaaa agcactgata tgaatgtaac agaggtagca atggcaacag gctttaatga
    18601 tataaattat ttcagcagaa cttttaaaaa gtataaaaaa atatctcctt cacatgttac
    18661 aaaagtaaat tgacaatcac actaataact ttattaatgt atccagttga ggaacatttt
    18721 tcatagtttc cattcggcat attgcttcct gtcttattcc tgtaagattt tccaaatctc
    18781 tctgcgacat cccgagttct tgccgtcttt caataatctt aattattata tcagacataa
    18841 tgtcaatttc ctttttctct tcttcaatta aatttaaact ttttagctat agcacttagc
    18901 taacattaaa aattttaact gatattgtag ctaaactatt gacgccactt tatatattag
    18961 ctataatgta tttgtattag ctaaatacgc ttatatcagc taaatctaat tgaaatggag
    19021 tgatcttatg attgttacaa caacagaagt acagaataac ttcggcaagt atcttaaatt
    19081 atctgagatt gaagatgtta taatcagcag gaatggtaaa gtagtcgcaa aacttacttc
    19141 ctgcacaagc aacaaagatg actttacaat acgcgaagca gctcgggaat atatatattc
    19201 cggtaaaaag gtctcatatg aagaatttct tgaaatatca gagaaaagtg agctgcggta
    19261 tgaatatatt gatggggaaa tatacctgct ggcatcacca ttttatgcac accaaaaagc
    19321 tataaggcag ctttttgtcg aatttgcaaa ttggttcaag gataaaaaat gcgaacctct
    19381 tagtgctcct tttgatgtta ctctttttaa cgtgattggc gatatttata cagatatgga
    19441 tacatctgct gaaacagcca ccaatataca cacaaataat gatactgatg cctatgccga
    19501 tactgaaact gatactgata ctgatactaa taccgataca gttgataaca aaaataaaaa
    19561 tattaatgtt gtccaacctg atatcctggt tatttgtgat aaggagaata tcaacgaaaa
    19621 aggcaggtac accggtactc cttcacttgt tgttgagata ctttctgaat caaccagaag
    19681 caaggattta ataaaaaaac ttaaccttta tatgcggagc ggagtaaagg agtattggat
    19741 tgtgaacact ttttcaaaag aaatatatct atatgttttt aaggattata atattgataa
    19801 aatgatggcc tttaaaaatg gcgagagggc tgaatcagat atatttaaag gtcttggggt
    19861 taacctggag catgtctttg attaaatgtg cctaaggagc atattttgaa tttggggaag
    19921 tgtttaccaa ttatgctaat tacaggtatc gccgtgggga atattggaac aagcactagt
    19981 aaaggctcta tacaattata agaccggtgg agacccttta caatttccca ccggtcctgc
    20041 atagtaacac tctgattttg tatagctctg ttcaactgtg aaattcagga attgcacctg
    20101 cattattaaa aaatatgtcc tctttaacat aattaccaac ttctgcctct tcaacaagta
    20161 gaagggaact cttcaaacaa taaagagggt ggttggaacc atatactatt ctatgatgat
    20221 taatttcctt tataagtttt tctataacaa acaaccagtc tttaaaacca gaagtatcaa
    20281 gaaaaatatt ttttctggaa ttaatgatat cttttaaagc taatacttca ccaactcgta
    20341 tgctttgcaa tacaactgta ttttccgggt ggtcagccaa aaagtcggat aaattcagta
    20401 tatcaatatt cttaggctgc atcaggtaat taagcctctc atcttccatt ctcattgtaa
    20461 gaaaaagggg taatttgttt tccgaaagcg tatgacagag ctgtcttagg tacttattat
    20521 caagcttata accatggtaa cagggatata tccttacgcc tttaatatca aataatttta
    20581 ttcccctttt tatatcatca ataaacccgg gaacttcagg gtttacagtc aatatatgtt
    20641 tataaggtgt atctttaatt atctcatgta attcaatgtc accttcaaaa ggatcattat
    20701 aaaatatact gtccagagaa gataaatagc catactcaat cccattttgc atatgtaccg
    20761 tttttaaatc atcaaatttg ttcatacgga tctttctaaa gggccagtgc cccaaaagac
    20821 aatttgtatc tatttttttc ataattatat caactccatt cctgtcaagg tttttatcaa
    20881 actcagaacc atttccgagg ctggatgtgt ctccgttcaa agagaaccat cctcagtaca
    20941 ccctaaaact ttaaaggcat tcttataata tattaaatct ctttgctcag cagttaaatt
    21001 tgcttcttct atctgcccat aattaactaa aaatgatcct gtcatatccg taccaaaaag
    21061 tattctttcc gctccaattt gttctactgt gtagtccaca tcatctctcc tgaaaattga
    21121 tccggatatg tccacccaaa catttttata attctttata gacttgatgc catggtaggc
    21181 attcccccca agatgtgcca taataaacct tgcatcagga tatctgcggg ccagttctgc
    21241 agtatgtggc ccagttgttt caaattccag ctgtccgaca gcctttttaa atgaatgtat
    21301 aagtacagga atgttgtatt ctatacactt ttctacaaga gggaaaacca gcggatcatc
    21361 acaataagta gcgacccata atttcatacc tttcatacca tattcttcaa tacccttctt
    21421 taaaacatca atgcagttat cattaacagg attgacatag caaaatccgc caatgtagtc
    21481 aggttgttcc tttgaaaact tatggacttc gtagttgagt tcgttaattt catccttgtc
    21541 ggagtaataa ctcctcaagc ccgaaatata tactttcgat atgttataca attcacatgc
    21601 cttcataatt tcccttttgt tattctcata gttgccgccc caaatatgtg catgtatatc
    21661 aattatcaca gcaaacccac ccccagtttt ttgttaataa aaatctcata ataatataca
    21721 ccaattatgt atacttatta tatccctttt taattaactt ttacatcctt ttgatttata
    21781 taatttttaa tatatcaata accaaattat gtcaatgcac tttgtttttc ttatttcaca
    21841 ttctatactt gtcttttaat tttatcatat tcattaagca gctcatcaag ttccttacta
    21901 agcttaataa tatcataatc ctgcaaattc cctttgagct ctatcattct atctaattct
    21961 tcctttattt tccgtatttt aacaattaat tctttggttt cagacatcag attctccctt
    22021 cctgttttaa tagttgcttt ttaagcttct attgccaaca ataatgcaaa aacaatatta
    22081 taatatttcg gttaatgtat ttagatttag gtaatgaaca acaatttatg tatggtatat
    22141 tgtgggaata aaatgtttga tttctaaaaa taatgggaaa ttatataaac aggcattact
    22201 atcgtatgcg gtgcgtataa cacttggtaa atatcatata ataatgttat taaccgatat
    22261 acatatgcat aaaaggtagt gttgactaac ttatgaaaga ggtgctttta tgaaggcttt
    22321 tgttgataaa gatgcatgta ttggctgcgg attatgcgaa tcattatgcc ctgatatttt
    22381 taagcttgaa gatgaaggga ctgctgttgc aattgatgag gagcttactg acgaaaactt
    22441 ggattgtgca aaagacgctg agtcccaatg ccccacagaa gctattgttg tggaatgaaa
    22501 accagcagaa cctaaaacca attcgggtgg ttctagaacc acccgaatta tgtgaaatta
    22561 tatatcaatg tcggatttta tatcaaatta attcttaaat aggatctccc ttaacgcttt
    22621 ggcattctct actgcttttc caaaagggtg attattaaaa gatacaattg taatatctga
    22681 tttaccttca acttcctgaa ttcttgtcac ccattccaga agttcgtctc tgctataaag
    22741 atagttatac ctttcggaac cttcgctgtt ataccatttt tcagcatttc tcccgtgcat
    22801 cctaatatat gatattttag atgtaacagc tagtgtattt cctattaaac ccttaatacg
    22861 gggttcatcc acacaaacaa atcccaggtt ttcatttttc attagatcaa ttacttcttt
    22921 ccttatccaa ttattgttac ggaactctat acaaatacca ataccttcaa accattctcc
    22981 aagagtttta aggtaatcca tattctctgg tttaaaatga aaggagtaag gaaactgaac
    23041 caggagacat ataagttttt tactttccag aagaggttta attgcatatt taaattgctc
    23101 agcttcaaca gtacctgcag ttttctcatg ggtaaaacct ccgaataatt taactgcaaa
    23161 cctgaagcta ttcggggtct ttctatttaa gctttcaaaa agtcttaaag aaggcatatg
    23221 ataataggta gaattaacct ccgtaaagtc aaaatacctt gaataatatt ccaacatgtc
    23281 accatctttt attcctttag ggtaaaatgg ccctatccaa tccttatatg aatatcccgc
    23341 agtacctgta tagatcataa ctcgtacgta cctccgctta ttttttggct ttttgtccct
    23401 tttatttttt gatttttggc ccttttccag ggaaatctct gcatcctgca aattacttca
    23461 atttataact ttaacttata tttcaagcat ctggcttata gtaacaaatt catagccttt
    23521 atcctttata tattttatta cttccggcag tgcctttata ctattttcca tagcttcctt
    23581 ttttccactg cagtgcataa ggataattcc accagggtat agctgctttt taaccctgct
    23641 cattatttcg gaagatgata ttcccgacca atcctgtgtg tctacagacc accttatatt
    23701 ataataacct aatgaattga ctacctcatc agctttatcc ccggacgaac caaaaggaac
    23761 tcttaaaata ggctgatgtt cttccaatat ggaggaaatt agttcttccg tgtctaatac
    23821 ctcatccttt attttgtctt ccgaaagctt ggcaaagttt ttatgacccc aactatgatt
    23881 gccaatatca tgtccttctt cccttatacg ttttaaaact ttagggtact tttttacatt
    23941 aaccccaatt acaaaaaatg tagcttttat attatttccc tttaaaatat caagaactct
    24001 taaagtaaaa tgctcatccg gtccatcatc aaaggtaata gcaacctggt ttttgctttt
    24061 attaccgtta tatacaggtt ttaagtctat gctaagcttg tcttgagctt ggtttccttt
    24121 ttcatccttg ctcgggctct gtaccttttg cccatctttt ttaaaactac tttcagcact
    24181 agttttacaa gctgtctcat cacctgtttc ataaccaact acattaccaa ccttattatc
    24241 agcttcatta ccagttatac tatcaactat attatcagta ttgttatcag ttttactatc
    24301 tttttcatta agtaactttt cacccgcttt gctttcttct tgtaatttta tatatctatt
    24361 gtcaatcagc ggtaaaaggt ggtaatattt tctataatta tttatcatat aaaaaacttc
    24421 tgccgagttt ccttttaggt tgctatcaac catatttcca tactttttaa atgctccaaa
    24481 gccaatctta tgattttcgt ttgacgcata ttcaataaaa gcaaatgata atgctataaa
    24541 taaaactatg ataatttttg ctgtaaactt tgataaaaca ttagttttgt ttgcgttggc
    24601 attaattttc atattcagcc tctaatttat ataataattt ctactaaaat atatgcattt
    24661 attaccactt ttgaactata tttaaaacta ttttatatta atactacaat gcaatgattg
    24721 aaagtaattt ctatttgcag tatacaaagt agtactaact tattatatgt taacttgctt
    24781 taattactaa ttatatatta atacattaat accacgaaat agttaaaaat aattaaaaag
    24841 aagtagtatc cactaaaata ctacttcttt tataaacaat cacattaatt attatacaat
    24901 agtcaaaagt gaaatcattt tcccggtatt ttacctttat tgtcttgctt cttactgctt
    24961 tcctccgctt tcttcgatat ataactattg aataccagtg caccaactag tgcagctgca
    25021 cttataacta taatagctat ttcaagagca cccacgaaat cacctcctat ccgaaaatta
    25081 taataggcaa caggcgaaag tgctcttgta cttactccca aagaaatata cagtagtaaa
    25141 gtcagatatt tcctttctaa catcagtcaa tataatttta tccttaaaag gttggttttt
    25201 atagaacaaa aaacagcttt ttacaaaatt tgtagtgtac atgcggttta taaggagcct
    25261 ggaagctttc ctccatgctt cctgatcagc ataggaaagt acagcatggt ttgcttcagt
    25321 attgattatt gctatatcac ttttatagta gtacttcctg tttccggcaa tacaagctct
    25381 ttgcaacaag gaagtttgaa gcaatataac tacaagtata aagactgcta aaagtttgta
    25441 agtccaacca tatctcctca tagttttacg gtctttaaaa ccccactttc attaaacaat
    25501 tacataataa ggatttacta atatcattat ataatgtctt tcataataag tccatataat
    25561 gctatagtac aaggtgcagg atacacatca gatttttaaa aatactttta ttcaccgatc
    25621 tttattcccg ctgagattct tattcaacgc atggcatata ccaactgctt tctcaacatt
    25681 caagataaat gcaatccctt tacccgtttt gttaagttca gcatcctttg ttattgaatc
    25741 taatactttt tctgttatgt tatctggaac gagggttagg actatttctt tttccggctc
    25801 aataggtatt ccaagtattt ttaaattctc atggattcct gtgccccgtc ctcccataat
    25861 agttccacct tcggcccccg ctttttttga agcttctact acactccatg ctttgccttt
    25921 atttacaata gttacaatta gattaaactc tatattccct tcttttcctg catatgaagc
    25981 tccgataata ttctcactta cacttccgct tgggctttcc tttaagtttt tacttgcatt
    26041 ttcatttact ctttcattta ctttttcatt catatataca agtcccccca tttcaaccac
    26101 aaatgcaatt cctcttcccg gtttatccag taaagattcc tttgttattg tctttaggac
    26161 atcatcagtg ttttcatcct tagctacaga aaatactatt tccttttccg gctcataatc
    26221 tattccaagt atctccaaat agacattctt atccgccgtc cctctcccca atattatggt
    26281 ttcgctgtca gcacctgcct tttttgctga taatgctact tttgtaccca ttcctttttt
    26341 aataattgta attaccaatt tataattact tccaattgac aatttgttct cactccttat
    26401 cattcttctc ctttttagaa taaacatatc ctaacaataa taccgataat attgaagcaa
    26461 gagcaacaag agataccata ccaaacccat ccagcattgg attccctcgt cctaatccct
    26521 tggctgctcc aattgttaat gccaatataa aagttacagt cataggtcct gtagctgccc
    26581 ctccagaatc gaaagctata gcaacaaaag tcttatcaac atgacgtaaa aggaaaaatg
    26641 ccaataaata ccctggaact aagaaatacc acaaagatat tcctttaaga actctcaaca
    26701 tggccagagc tatggatata gctactccta aagataaaga ataaagcatt aatgttttat
    26761 taatatgccc ccctgacacc ttctcaacct catcatttaa aacacgcact gtaggctcgg
    26821 cataagcaat tgaaaaccca gcaagaaatc ctataggtat aagaatccaa ttatttttca
    26881 tttctccaag tttaataccc atgctttcac ctacaggcag aaagcctata ttcacgccct
    26941 gcagaaaaaa ggaaagccca aaaaaagaca aaacaactcc tgctgctatc cgttttaatt
    27001 ggtgcttact taatctcagt acaaagatct gaaaaataat aaatattata agtatgggaa
    27061 gcaatgcata aaagacttcc agcattatac cagaaaagcc tttaaataat gagcttatca
    27121 acgaaccaac acccccaata tcaaaactgc aaggataggc cctatacacg ctaatccaag
    27181 caagccgaaa ctatcacttg aggaatcttt cttagcagtg accgacatga cacctacccc
    27241 caatgaaaca ataaaagaaa caatcatagg ccctgttgct acacctcctg catcaaaaga
    27301 aagagcaaaa aatctggatg gagcaaataa cgaaataata aaggcaagag tgtaccctcc
    27361 aattaaaata tatgtcatag gtaaattaat aaatatcctt gttaatgcaa gagaaataaa
    27421 tataccaact ccaatagcca cagaaataat taaaagcagc ctccctattt caccttctga
    27481 aacacgggta acctgcgaag ccagaacctg tacatcaggg tccgcaacag taacggaaaa
    27541 acccagtata aacccaaata tagttattaa ccatagtttc cctttactta ccagggccga
    27601 acccagcatt tctcccatag gtataaaccc agctttgaca cctatcatga acaatattaa
    27661 tcccaacatt accataacag ttccgccaat aaacatcaca aaatcttctt tgggcaactt
    27721 tacaattgtc aattgcatta ttgtaacaag tattgtaaca ggaagaatag cataaactac
    27781 ttcaagtacc gtttctttta tgttcaaaaa aagcaccccc aatactacag tgtgtacgaa
    27841 atagtattta atttaattag tattcaattt agttatttca ttttttagtt ttgcgaactg
    27901 tgtttataaa tttttacaga actttgatac ctatgataat tgtaatattt attccattct
    27961 attagcaaaa gcaatgtttg tcaaatacct ttaatgggtt ggggtgcatt ttttattatt
    28021 aggcagaaaa tttatacttg cctgtttgct taaataaggt acaaatcatc aactaaatta
    28081 atatactgta agcaggatac attatgtata ccaaaagtgt aactaaactt tctgatcgtt
    28141 tatatgaaca actttttgta caggagtgat aatttttagc ataggagtga tatataatgc
    28201 cattatctgc aagagagctg tttgcaagat tactaaaatg tgaagctgat ggtgaaggta
    28261 tcgatggcat gaaggcagtt gcaaccgttg taatgaatag ggttcatgtt gcttatggtg
    28321 aatatctcag agaatgtgag ggtgatttgc gccgggttat tgagcagcct tatcagttta
    28381 catgcatgct tacccaggta tatggccagt tcaaccccca aaatatctgg gctaccgctc
    28441 ccgaacagat acactatgat attgcagatt gggccttgtc aggcaacaaa caccagggtt
    28501 caaatgactg tttatggtat atgaacccct ttagcccaac atgtccaaca atattcccat
    28561 ataatgggac aggaagcatt tttaatagaa tcggacagca ctgctttttt atacccacag
    28621 agctatatgc acaaacctag tgacattttt atttcataca ttaatactac tactatccaa
    28681 atttggaaag tgatgtattg cgagatgatt tttgatagca ttgggaagat gcattcctta
    28741 cgggtcagca aaaattaccc tctagccctc tagaaatgcg ctttccattg gcaacataac
    28801 aaagtagtac taaattgtag ttaaaacata aatttttata ggagggattc atgtgatgaa
    28861 ttcaagaaat tcttgtggta atgcaagtcc aggaatgcct gttccatata tgcagagtac
    28921 tgcaatatct cccgggtatg agcccaagcc ttttcagcct tcaggcgctg ttcccatacc
    28981 catgccgtca ttacccacag gtataccgac aggtcctgca tatgggggaa cagttcctac
    29041 tactcctatg ccgatacctc aaccacctgc tcaggctccc caaacagtat caagtcctta
    29101 ctatttagca ggattcttgc gcaattatgt cgggagaatg gtaagggtag agtttctaat
    29161 gggaactaca gcacccctgg tcgatagaat aggcaacctg atggaagttg gtgccagtta
    29221 tattgtacta aggcctgtag gaacagatga tctaatagtt gctgatttat attccataaa
    29281 atttgtaaca atttacagat aaacatacta taaagattat gcaagggaat atataatgca
    29341 aagggacaca ccttttaaga caacagataa ttgttatcga aggagtgtcc ccattaatca
    29401 tttgcattac ataactacac caggttttca ttcaaataaa aataactatg aataattatt
    29461 ctctttattc aatatttttt gaaaaaacga ttgccatctc aaaaaattcg tatataataa
    29521 atatatattt ctttagttag aaatacggga aataattaat tgagtaaaat taacatgaat
    29581 gccaaatacc agatatgcat gcatattgga ttatagttta atggtatgag tatctggaaa
    29641 taaaataaaa aaataaatag ataaacaaat ttaaaggagt gggaggaatg aataaaatga
    29701 gttcattaag aaaaacacta agtctttttc ttgtattagg tttaacaact gtcttttttg
    29761 caggatgcgg aggtgaaaag acagcagatt catcaaatga gataaaaatc ggtataaact
    29821 acgagctttc aggtaaagtt gcctcttatg gtcaaagtag tgtagatggt attttaatgg
    29881 cctttgagga aattaatgcc gcaggcggcg tgcttaataa aacaatcaag ccggtacaag
    29941 ccgataataa atcggaccct gctgaagcta ccagcattgc aaccaggctt gcaacacagg
    30001 acaaggtagt tgctattcta ggacctgcta catcaggtgc atttaaagct acaatccctg
    30061 ttgcaataaa aaataaggtt ccggttttgt ctgcatcgac tactgatgat gctgttactg
    30121 tagatgaaaa aggagttaag gaatatgctt tcaaaatttg cttcaacgac tctttccaag
    30181 gaaccttgat ggctaatttt gcaataaaaa atttaaatgc taaaagtgct gttatacttg
    30241 ctgataatgc aagtgattac gctaaaggtc ttgctaagaa ctttagagaa accttcacaa
    30301 atggcggtgg caaaattgta ggagaagaag gttatgtggc aggtgaacag gactttaatg
    30361 cagttctgac taagattaaa gggttggatt ttgatgtaat ttttattcct ggatattatg
    30421 aggaagtagg cttaataatt aaacaggcaa gagatttgga aatagacaag cctgttcttg
    30481 gagcagatgg ttttgattcg ccagatcttg ttgaccttgc aggtaaaaaa gcactcaata
    30541 aggtttattt ctctaatcac tattcatcat tagataaaga tcctgatgtt attagtttta
    30601 tagaaaatta taagaagaaa aatggcaaag aacctaatgc atttaatgca ttgggatatg
    30661 accttggata ctttattgcg gatgcaatta agagagctga ttctacagat cccgtaaaga
    30721 taaaagaagc ccttgccgca accagcaact ttaaaggtgt taccggaagt tttagtatcg
    30781 atgataatca taatgctgtt aaaactgctg tagttatcga gttaaaagat ggtgaacagg
    30841 caagtgcagt taaagtgtca ccataaaaaa atatatagag agagatatat attttttaaa
    30901 atgagaaata aaatagcata taaaactaag agagataaaa tagacatata aaaaaaataa
    30961 gtatatacta taggtgagag gatgttttgt cctctcactt gtttaatttg ggggtgtaca
    31021 agatttgaca caatttctgc aacagttaat aaacgggctt tctctcggaa gcatatatgc
    31081 attaattgct ttaggatata ccatggttta tggcataatt aagctgatta actttgctca
    31141 cggcgatgta tatatgcttg gagcatatat tggatatgcc tgtataaaat attttaacct
    31201 tagctttttc ccatctctgt taattgcgat gacagcctgt gctcttatgg ggtttgctat
    31261 cgaaaaaatt gcatataagc ctttacgcaa tgccaccaga atagccgctc ttataacagc
    31321 cataggtgta tctctttttc tggagtactc taccatgttt gtagtcaaag ccaatgtgag
    31381 gacatatccc agtgttctaa atgaaattag tttcaaacct ttacaagggg ttatagtaaa
    31441 tatgaagcag gtgtacattt tcgcagtgac tattatactc atgataattc tacaatatat
    31501 tgtacgaaaa acccgcgttg ggaagtctat gagagctgtt tctacagata gggatgctgc
    31561 cctcctcatg ggaatcaatg ttgatcgtac aatatccttt acctttgcaa ttggttcagc
    31621 tcttgccggt gcagccggtg ttttggtagg aatatattat aattcaatta atcccttaat
    31681 gggcatactt cccggactta aagcatttat agctgcagtc tttggaggta tcggcattat
    31741 acctggtgcc ttagttggag gaattacaat aggagttatt gaaactatgg taagcggtta
    31801 tggcaattct ctttataaag atgccgctgc ctttgcaata cttattatta tccttttgat
    31861 aaagccctcc ggccttttgg gtaaaaatac gagggagaag gtgtaggtta tggcagtaaa
    31921 ttctatagat atgaaatcaa atccaaaaat gaaatggtta aaaaatatta tagtactcgt
    31981 tgcgttttat gtaattatgc aagtactaat atctttaaat ataattaacg attactatca
    32041 ggtaacaatc gctatgatat gtataaatat aatacttgca gtgagcctta accttataac
    32101 aggttttaca ggacagttct ccctgggaca tgcaggcttt atgtcaatag gggcgtatac
    32161 ttgcgctctt tttacactga acatgagtca gcaatattcc tattttatag gaatattcgc
    32221 aggtgctgta atggctgcaa ttatcggggc acttatagga ataccaactc taagacttaa
    32281 gggtgactac ctggccattg ctacattggg attatcagaa ataatcagga ttattatctt
    32341 aaactttgaa tttaccggag gtgccagtgg tttaagcgat ataccacaat atacaaactg
    32401 gacatggtta ttcgtattta ctgcaggcac agttataata ataaataatt ttatcagatc
    32461 atctcatgga agggcatgta tttcaataag agaagatgaa attgcagcag aagcatctgg
    32521 gataaataca acaaagtaca aagtgcttgc tttcgtaata ggtgcatttt ttgccggtat
    32581 tgcaggagca ctttactctt catatttcta ctttgttaaa cctgatatat tcggatttct
    32641 aaagtccatt gatatcctgg taattgtagt ccttggcggt atgggaagca tttcaggttc
    32701 tatacttgca gctgtcctgc tggctattat atccgcatac cttcaggcct tcccggatct
    32761 caggatggtt atatattcaa tattactggt aataattatg attttcaggc cacaaggatt
    32821 aatgggcaca aaagagttat ctctaaactc ttttttaaaa aaacggcctg caaaagttga
    32881 gtgaacgagg tgattatagg atgtctattc tttcagttaa aaatcttaca atgacatttg
    32941 gcggcctggc agctttgaca aatgtagccc ttaaacttga gaaaggtgaa ctggtaggac
    33001 tcataggacc aaacggtgca ggtaaaacca ctgtctttaa tttgcttaca ggagtttatg
    33061 tacctacttc aggctctatt gagtattgtg caaaggattc aatgaagaaa attaacgggt
    33121 taagccctca taccatatct tgtatgggaa ttgcaaggac ttttcagaat ataaggctct
    33181 ttaaagacct tactgttctg gataatataa agattgccat tcatcaaaat ataaaatatg
    33241 gacttgtacc agcttttttc aggacaaaat catttcatag ggaagaacag gagattactt
    33301 ccaaggcaat tgatatactg gaaattttca accttggagt aaaaaagaat gaacttgcca
    33361 gaaacctgcc ttatggtgaa caaagaagac ttgaaattgc aagagcactt gcaacaaggc
    33421 cctctatcct tcttcttgac gaaccggctg ctggaatgaa tcctaatgaa accgctgagc
    33481 ttaccaagct tatagagtgg attcggaagg aatatgaact tacaatactt cttattgagc
    33541 atgatatgtc tctggttatg aaaatttgtg agagaatata tgtgctggat tatggaatgg
    33601 taatagcaga aggcaagcct gaagaaataa gaagcaacaa acgtgttatt gatgcttacc
    33661 tgggaggtga agtactggat gcctgaaaca aataatgttc atcaagctga taatattcaa
    33721 gctgataata ttctggaaat aaataatatt gatgtatatt atggagtaat acatgccctg
    33781 aaaaatgtgt ctttaaaggt acggaaaggt gaaattgtaa ctctgatcgg tgccaacgga
    33841 gcaggtaaaa cgactacact aaggactatt tcaggcctga taaagcctgc aaaaggctcc
    33901 gtatttttta acagtgaaaa gattagcggt ataagcgccc aggaaatagt aaaaaaaggc
    33961 ctttctcatg taccagaggg tagacgcatt ttccccgata tgacagtaat ggaaaatctc
    34021 gaactgggag cctttttacg taatgataaa atcgggataa aaagtgacct tgaaaatatc
    34081 tattcaaggt tcccaatatt aaaaaagaga aaaagacaaa atgcaggaac attaagcgga
    34141 ggagaacaac aaatgctggc tataggcaga gccctcatgt ccaagcccaa actcctttta
    34201 atggatgaac cttccatggg gctggctcct cttttggtaa atgagatatt ctcaataatt
    34261 aaagaaataa atggcctggg aactactatt ctattggtgg agcaaaacgc taacctggcc
    34321 ctttccattg ctcacagggc ttatgttata gaaacaggct cagtagttct tgaaggttcc
    34381 ggcaaggaac tggcaaaaag cgaagaaatt aaaaaagcat acctgggagg ttgaggtttt
    34441 tatgaatgtt aaaagcagaa tgacaagtaa tccatacaca atttcacctg aggccacaat
    34501 agctgaagca ctggaactta tgcgaagcaa taatgtaaga agacttcctg tagtaaggaa
    34561 cggaaagctg gttgggatag taaccgagag ggaaatccag gaagtttcac cctcaaaagc
    34621 tactactcta agtatttttg agttaaacta cctattatca aagaccaaag ttagtgcagt
    34681 aatgaccaag gaggtaataa caatttctcc cgatgcactc ctggaagaag ccgcagttct
    34741 tatgagagat aacagagttg gtgctcttcc cgtcattcag gatggcaagg ttgtaggtat
    34801 tataactgaa actaatattt ttgatgcctt catagacctg ttgggtttca gagaaagcgg
    34861 tacaagaatt tgcgtggaag tagaagactc gcccggaatt ctcgcagatg tagcacgggt
    34921 aattaaaggt tttggagcaa atattactca tctcgtagta tacaggagta ataccggtaa
    34981 aagtgatatt ataataaggg taaacactac aaatactgat gaaatgatcc aatcccttga
    35041 aaaacagggg tacaaagtga cttctgtatc gaaaaactat taaatctttg tgatggttct
    35101 tttgaagcat ggaaacatag ggacagttct cttgtttcgc ttttgtttcg attaaattga
    35161 ggagatctca gagggaaatt ccatagaaag attaagaaat ttcccttaac tctcttctga
    35221 gtgcatttaa cttaagtgaa gaaaatgtac tcctctttta tttacccttg ctacatcttc
    35281 atcaacaaag ccttgaattt atcaataatt tcagcttgct gttcaatatc catattgctg
    35341 ccaaagtagg gtgaaaagga aaagtatccc ttgaatcccc tggccagcat agctgatgcc
    35401 atttctttaa cctctgcatt tccttcccct gggaaagtgg ggcttccatc aacataaagt
    35461 ccgtcattaa tcctgaggaa tagaatactg ctttttaatt tgctgttata aaacacatga
    35521 aaaaatggat gtttctttaa tctcacatat tccaaaggat taaatacaag ctggatgcct
    35581 tctaatccaa attctttgct aattcctgta aattcgatat catgagtaaa gcttacagaa
    35641 ctgttgtttt ccattaatac acctatacca taactctccc ccaacttaat tatctttttt
    35701 aaatgcattg caagcttttg aaagcaacta ttttctgtat catctgaaca acaccgacct
    35761 gcgcaactgc ttgtatcatc gtaacagca
//
LOCUS       DAOSDD010000011        35503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_613_length_35503_cov_5.456866, whole genome shotgun sequence.
ACCESSION   DAOSDD010000011 DAOSDD010000000
VERSION     DAOSDD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 35503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35503
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_35503_cov_5.456866"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..510)
                     /locus_tag="PK733_01885"
     CDS             complement(67..510)
                     /locus_tag="PK733_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09329.1"
                     /translation="MDSYTKKLEYLGSLANGIYAAAEIYNIMKLNESDSNKKKPYSKD
                     ENNQYRRSVNPSTTAMYNDVLIAIAKYLPEASYRKNNLRKSLDKSILYGNTYKQLKDN
                     MTSLRVDGMDKNKLINTLSIVKPLLNGERADIVDKFLKIYDIINS"
     gene            889..2061
                     /locus_tag="PK733_01890"
     CDS             889..2061
                     /locus_tag="PK733_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOJ09330.1"
                     /translation="MVSYKSFDEINEKIKTGKAVVVTAEQMIDIVEEKGIRQAAKEVD
                     VVTTGTFGPMCSSGAFINFGHSDPPIRMSKVWLNDVPAYAGVAAVDAYIGATELSESR
                     GMEYGGAHVIEDLIAGKDVHLYAESYGTHCYPRKEISTYINKNNINQAFMFNPRNAYQ
                     NYNAASNSSEKIMYTYMGTLLPKCANVTYSTSGQLSPLLNDPLYRTIGIGTRIFMGGT
                     QGYVAWEGTQHNPEQKRGENGVPLASAGTLSLIGDLKKMDTRFIRAAVFQQYGVTMFV
                     GVGIPIPILDEDIAEKTAVRNRDIYTNIVDYSVSSGPKPVLKKVSYEELMSGSVDLNG
                     KIIPSAPLSSMKKAREIAVTLRELIMAGEFFITQPISSLPLTNTVNKLEIREDGNR"
     gene            2058..2477
                     /locus_tag="PK733_01895"
     CDS             2058..2477
                     /locus_tag="PK733_01895"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ09331.1"
                     /translation="MKKIKVTMYFPSNKITAPVTYHLIKDYNLMLNILNADVSLNKIG
                     TLVVDIEGEEKDIEAGLEFVKSQGADYKVFSKSIIWQEESCVHCGACTAVCPSGALTM
                     DKTDWNLTFDKEKCLVCELCVKACPLKVMCVRNGDKA"
     gene            2483..3352
                     /locus_tag="PK733_01900"
     CDS             2483..3352
                     /locus_tag="PK733_01900"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOJ09332.1"
                     /translation="MKKSFYEKRSYRSLFKGENLSFFNICVYETDLTIGISEARIREN
                     NQAEDNQVKDSKVKDSQTNDSQTKGSQAKNSAGKELVQEDLLKTTQNLVRKYRQYIED
                     YIRIHPEFLTSLEPIIPKSGAAIIVNRMCEAAMKAGVGPMAAVAGAIGQMVGQELLKY
                     YEEVIIENGGDIFIKTNSVRKVGVYAGKSPLSEKIAIKIQPHHTPAGVCTSSGTVGHS
                     LSFGKADAAVIISRDAFLADAAATAVCNRVKCPADIEKALKFASEIEGVDGALIIIDD
                     KMGAWGDIEITGF"
     gene            3467..4300
                     /gene="proB"
                     /locus_tag="PK733_01905"
     CDS             3467..4300
                     /gene="proB"
                     /locus_tag="PK733_01905"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOJ09333.1"
                     /translation="MEDINIFRKRLYDAKRIIIKVGTSTLTYDTGKVNLSRIDMLSMV
                     LSDLVNQGRDVVLVTSGAIGVGMGKLKLAEKPGTIREKQAVAAVGQCELMHIYSKFFS
                     EYGHIVGQILLTRDVVEDEYRRNNVINTFETLLEKGIVPIVNENDSVCVKELEGENGD
                     SMVFGDNDTLSAIVAKLIKADLLIILSDIDGFYNCDPRKNACSRKIPVVAEITPEIEK
                     CAGGEGTQRGTGGMVTKVAAAKGVTSEGIDMVLANGEDPKIIMDILDGKDVGTLFVGR
                     N"
     gene            complement(4297..5382)
                     /gene="alr"
                     /locus_tag="PK733_01910"
     CDS             complement(4297..5382)
                     /gene="alr"
                     /locus_tag="PK733_01910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ09334.1"
                     /translation="MNKLIIEKDKLDKNIEIIKGLTQAKIIAVVKGNGYGLGVLEYTR
                     FLSEKGIDIFALSDVRDAVYISESGFSGRVLLLTPTNDDEEAEMIIKNRITTTIGSMT
                     SALTINNAAARLNETAQVHIKIDTGFGRFGFLPDEVIDACTFISTLSNIKVTGAYSHL
                     SFSFSKKPEHIYSQYRKFINSVEIMRQNGIDPGMLHICNSSAFLRFPQMHLDGVRIGS
                     AFLGRIPIENVYGLQRIGFLKSNIIETKELPGKHNIGYANTFKTRNPVRTGIVPVGYR
                     HGFGIEKSRDTFRLIDLLRYVYNDIKSFNKKSYVRIGDKSAQILGRVSMYNIVVDLTD
                     IDAKVGDEVVLDANPLLVPIDIEREFI"
     gene            complement(5426..6568)
                     /locus_tag="PK733_01915"
     CDS             complement(5426..6568)
                     /locus_tag="PK733_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOJ09335.1"
                     /translation="MIDNINTKNSSSELHGEIKSYTEYTEFLQHHPKGHFMQSPQWAG
                     VKSNWVNEIVAVRNSKGNIKGSMSLLIRKVPFINRTIMYSPRGPVCDIHDRQTFKELI
                     EQSKKLARKYKSYVLKLDPDIEKEDTEFENIVRDLGFKIKNASENFEGIQPRFVFRLD
                     IKGKTEAEVLMAFNQKVRYNIRLSAKKGVQVKIGSKADIPEFHKIMVETGIRDEFVVR
                     DPSYFETMLDCLGDNIRLYLAYYNEEIIAGTVAVVYGNKCWYLYGASSNEYRNVMPNY
                     LLQWEMIKWAIECGCDIYDFRGVSGNLDESNPLYGLYRFKKGFGGKFTEFIGELDYVF
                     NPFIYNLVEKGEKVFRELRRRLFILKNKGNNQSQNAQEKDNQSENT"
     gene            complement(6570..8054)
                     /locus_tag="PK733_01920"
     CDS             complement(6570..8054)
                     /locus_tag="PK733_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOJ09336.1"
                     /translation="MKKKLMTLITVAIISELAAITVVFYLFSYFLKDETIVNAVSADT
                     KNVLPGYSSIYSNRKSAAENSQSEKNHIDGRNIIEAYIDFVGDRVIIKEDLYFKVPDR
                     TEGNDINHVSNENDINCTSAVDKVYLYIPSLNAADTTIKNIFSNNDIINSELNGMVLE
                     IQLNRSRQDNPAGQVQNTDREISNTYISIDFEILLNNSTGTISRSCNGKCVYLANFLA
                     TPAVFAGETPVFMDKTSFGDPYIYDMNNYYITFKTDKDMEIYAPGIKEGIQNPTGNTR
                     EVSFTAKNLRDFSAVILNGEHDIHIEKVKNTNIYYINSSKANDYVREAFLFAVEKIGP
                     YPYDKLFVVKADIHPLKGMEFSNMVFLSDKCFTNKEDLQRVAYHEVFHQWFYGIIGTN
                     QINEPFLDEGMVNYLSMKLMNDNFGNTFDSRFLDMTLRDYGSRNDYYKLAYTDAAVFM
                     ANIHQKMGDDFYKLLQKIYNDKKFTILYYNEFEEYLRQFSRGGY"
     gene            8362..10494
                     /gene="mrdA"
                     /locus_tag="PK733_01925"
     CDS             8362..10494
                     /gene="mrdA"
                     /locus_tag="PK733_01925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ09337.1"
                     /translation="MEENFFKRVFKDRYNIIIMLLLLAGAAIIYKLVDLQIINGNQYF
                     EDSQYKLLMNKKIMAARGNIFDRNGVPIAINRVGFNVQIVNARLKEAELNSMLLDLCK
                     ILEKNGDNYYNSFSKYVTYDPLDFGSEAKKSQERLEKWIKDNYIKIEITGSIDIKVLF
                     KALRERYGIDKKYTDEEAYKIMVMRYEVRNYTSLNSVLLAKDVSTESVAEIEERHHIF
                     RGVTTDIEYMREYINANLASHVIGYVRGIDADTYNKLKNEGYSINDIIGKTGIEYSAE
                     KELRGTPGTKKVEVDTRGRLTRVIEETPAIPGNNVVLTLDMNLQKIATGILEKRIKEI
                     RLLNGLYHFHDAFAGAVVAIDVNNGEVLAMASYPGYDPSIFLAGPENKEAQQAIMDLN
                     DPSQKEITSEYNRAISGRYAPGSTFKPLIGIAALEEGVITPSTVIYDKGYVTYDGMRF
                     TCMDWRNGKGAHGAITLSHALATSCNIFFHEAGVMTGIDNIDKWAAYFGLGVKTGIEL
                     PGEIEGIRSNKEYKKQVSPYIWGKADTASSSIGQLYHEFTPLQLANYVATIANGGIKY
                     KPHLIKCITKCDGKILKETEIEYEQIPVKEETMEAVKKGMVAVANSVDGTAVNVFKDL
                     PFKVGAKTGTAEAYREGQSNNGVFICYAPAELDKTPQIAVAVVIEHGVYGMYAAPIAR
                     EIISEYLELNSQSVDGIESAPAVPVFVP"
     gene            10501..10773
                     /locus_tag="PK733_01930"
     CDS             10501..10773
                     /locus_tag="PK733_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09338.1"
                     /translation="MKHTKEKDSSFVKKGWFSVLLLSKSKQKNRPLFSFLVLLCCCCY
                     RAAFLVGTAISDAITAAAFLVGTALPDAITAAAFLVDTALPAAASV"
     gene            complement(10718..13336)
                     /locus_tag="PK733_01935"
     CDS             complement(10718..13336)
                     /locus_tag="PK733_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOJ09339.1"
                     /translation="MNQNRAEHSRVPAKRRKKFRRIKKIPNSSRINRSASANIDNGAR
                     KHAGKAKLLSAGRYALTTCKVILLIFTAICCAGAGVLGGAILGYIRTAEPLTKEQLNI
                     KIETSYIYDSKDNEIAMLTGSLNQNREPVFYKDTPEYLRKAFIAIEDERFEKHPGIDF
                     RRIASAILGIVTGSSSHGGSTITQQVVRNITNKTKRSVERKVQEWYLAIKLERVLEKW
                     QILELYMNVIYMGNSCHGVQSASRMYFGKDVKDLSLAECALLAGITNSPTNYDPYNTY
                     IDSGRENAVKRQRIILKKMLELGFINQNEYDQALQEELKFVEKDESDKEIKVQSYFVD
                     YVINEVVNDLMEQKGMSKQMALTMVYSYGVLIHTTMDPDIQKVMDDVFMDEKYFHQNK
                     EVTQHPQAGMVVIDPNNGNILAIRGGYGEKKASNILNRATQIERPAGSSFKPLVDFGP
                     AIDRRIITAATIFDDAPVYFLGLDKKRYPENYTRYFEGLMTTRYAVKRSVNVPAAKTY
                     LMFKDLNIPLEYLRKAGIDRNQKNLSISLGGLEKGVSPLQMAAAYVPFTNRGIYYEPV
                     SYTKVTDKDGNIILEKKPAYNAVYDEATAFIMTDMLKDVCRSTRRPGELGGTAAKLGT
                     IKNAKNEIIPTAGKTGTTDDDKDRWFVGYSKYYTGAVWYGYDHPTPIVGVPNNDNPAA
                     LIWNAVMQKIHRNKAPLDFPIPSNVVKKKICVYSGKIATDACTHDPRGDASFEEYFIK
                     GTEPADNDFCNAHIIAKVCKDAKDIWGHNLLAGPDCPQDSIMERVYVARKIPFRPMKP
                     GDPYPLDWKYEYPAGEYCNVHGYSSSSTTSTTNSQSQYYEYMDVPQQFNHQGENEQLT
                     QMQQQEELYQQEKQQQ"
     gene            13933..14607
                     /gene="yunB"
                     /locus_tag="PK733_01940"
     CDS             13933..14607
                     /gene="yunB"
                     /locus_tag="PK733_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HOJ09340.1"
                     /translation="MWIRNTMLYRRFRYVKKRHTARIHIYLRFVATIILFVIIVKYAE
                     KTILPYMSNISEYRVKSAINILVNEAVQKTFSEKQRYDELVCINKNEKGEIEAITIDT
                     PKINMLSAKIAQEIQQKINSRDTVLIKIPIGSLLGETILYNSGPSIYATVRQNGNIET
                     EFISEFLEAGINQTKHRIVLSVKINVIVHTTFIKKNHIIINSIPIAETIIVGKVPAAY
                     YAGGGK"
     gene            complement(14840..15235)
                     /locus_tag="PK733_01945"
     CDS             complement(14840..15235)
                     /locus_tag="PK733_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ09341.1"
                     /translation="MFKKKNTGTSSAGSFNSLIGDNTIFEGNINCQGAIRIDGKVTGD
                     IKSDDDIFIGETAIVKGNLYGNAIYVSGMVKGNIYSKSNIRFFSSGSIHGDIEAFSII
                     TDEGAIFNGKCNTLVSEDSREAVPQEKGN"
     gene            complement(15317..16438)
                     /locus_tag="PK733_01950"
     CDS             complement(15317..16438)
                     /locus_tag="PK733_01950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ09342.1"
                     /translation="MVNETEPSIKNIINMKRNRVNKIKRKNVNKVPLKFSMVGPINLI
                     KLIKAGFELRRQKRLSKSSRFVKGAAEDNKKYFSITIVPHSSNDVKTYKVSNLYTKSA
                     VSLTIFAVLSVSASLYFTGLVNENRLLKAGLNEMYNLNAQQKAILSKRSDEINTLLTE
                     ENNANLSISELTEKYREIVDKYVSGRINGGLASRSGDRSDSTFVNDIEELRGILTELE
                     KANNLKEEKLGDLTETENTLRKYLDCLPTQWPVNGDISSKFGQRKDPISYRSSFHKGI
                     DIAASYGTNIKAAGDGKVILTGSYNEYGLAVIIDHGNGLATVYGHTSKILVKKGQKVE
                     KGEIIAKVGSSGRSTGSHLHFEIRVNDNPIDPYKYLDKR"
     gene            16974..18413
                     /gene="rlmD"
                     /locus_tag="PK733_01955"
     CDS             16974..18413
                     /gene="rlmD"
                     /locus_tag="PK733_01955"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOJ09343.1"
                     /translation="MGRINGIDNIRNKKTDVVNKDIAPANAVEKNKNYVVEITDMNHE
                     GQGVGHIGSLAVFVDGGVIGENIELKVIKVAKNYAVGKLLKILRVSPGRTEPFCSIFK
                     SCGGCSLQHINYNDQLQYKTNLVKETLKRIGKIDEAIVHDTIGMDHPFNYRNKAQFPV
                     GLCDGKPAIGFYAKRSHEIVGSCDCGIQDPVSDKIREIFRRFIIDNEISIYDETTHLG
                     LVRHLMVRKGFRTGEVMVVVVINGKDLPYRRKLVDDLISRVPNIKSIVLNINTQNTNV
                     ILGERNMKIYGDDTIVDYIGDFKFHISPLSFFQVNPIQTEVLYNKAMEYAQLTGRETV
                     FDLFCGTGTIALFMAQKAGKVYGVEIVEDAILDARKNAKLNRVGNAKFIAGDVEKVIP
                     ELYGQGVRADVVVVDPPRKGCETSLLDTIADMGPERIVYVSCNPATLARDLMYLEGKG
                     YSTAEVQPVDMFPWTVHVETVVLMSRVKE"
     gene            18705..19073
                     /locus_tag="PK733_01960"
     CDS             18705..19073
                     /locus_tag="PK733_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOJ09344.1"
                     /translation="MNLKERARQLKTDIPAVFLSLKSKDTPLMAKILAVITVGYALSP
                     IDLIPDFIPILGYVDDVIILPVLIALTIKLIPQDTFERFRKEAEGMWQSGKPKKWYYA
                     IPIAIIWLLVIWLIVKAIFF"
     gene            19112..19837
                     /locus_tag="PK733_01965"
     CDS             19112..19837
                     /locus_tag="PK733_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ09345.1"
                     /translation="MEIWMFVILILSSFVAALISGAAGFGGSLLLLPVVTAYVGSEVA
                     VPVLTIAQLIGNLSRMAFGLKQIEWKSVGWFSLASLPLAALGAFGFSLLPKDIVTRCI
                     GAALILLVLIKLMMKRDLPKGKGTLLIGGAVTGGLSGLCGSGGPIGAAVFLSLGLTPV
                     AYIASEAATAMAMHILKTVIYSQLTNMTIKTFMVGIAMGAAMLVGTFSANRFIKKMDK
                     NKFQKYVAVLLSVVGIYMVITGA"
     regulatory      19891..19969
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            20022..21212
                     /locus_tag="PK733_01970"
     CDS             20022..21212
                     /locus_tag="PK733_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ09346.1"
                     /translation="MLFSLALMVLCGLVLSGTMQKLKIPGLVGMLLTGIVLGPYALNL
                     IAPELLNISADLREIALIVILTRAGLALDINDLKKVGRPAILMCFIPATFEIAATTIF
                     APMFFPISHLEAAIMGTVLGAVSPAVIVPKMLKLMESGYGKSKSIPQLILAGASVDDI
                     YVIVLFTSFMGMYSGSSFDIGSLVKIPVAIVVGLLIGILVGLALVKLFKIIHMRDTVK
                     ILILLSTSFLLVTLESVIKAYVPMSGLLAVMALGGTILKQYGILAERLSGKFSKIWVA
                     AELMLFVLVGATVDISYAARAGIAAVALIFIAVIIRVCGVFVCLIRTKLNTKERLFCA
                     IAYLPKATVQAAIGGLPLAAGVAAGNTILAVAVLAILITAPLGAIGVDASYKKLLTRG
                     DSSV"
     gene            21205..21576
                     /locus_tag="PK733_01975"
     CDS             21205..21576
                     /locus_tag="PK733_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09347.1"
                     /translation="MFRPLRLLNDSKTVSLNGQLKRGFHYAILPSQTGLCRYIITVNH
                     GELRLNHMAYNPDGGTWSCPPQNRQQAHYDLKQGEKYEIQLVIGESFNKPDIVVIVNK
                     SMLNGAEFSYIFKYGGDESKI"
     gene            complement(21573..21932)
                     /locus_tag="PK733_01980"
     CDS             complement(21573..21932)
                     /locus_tag="PK733_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ09348.1"
                     /translation="MITYKTAESVCMGHPDKLCDLIADNILDACMRKDKASRVACEVM
                     ATKGKIIVAGEITCSGKVDIRLVVKNVLREVGYNSWKFTVFVFVHQQSADIAAGVDNA
                     LEARNGINDQHRVPFLF"
     gene            complement(21946..22497)
                     /locus_tag="PK733_01985"
                     /pseudo
     CDS             complement(21946..22497)
                     /locus_tag="PK733_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
     gene            complement(22593..22961)
                     /locus_tag="PK733_01990"
     CDS             complement(22593..22961)
                     /locus_tag="PK733_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOJ09349.1"
                     /translation="MPYKPKRPCAYPGCGRLAVREQYCAEHQKAMDNHYNQYERDPAS
                     NKRYGRAWKRIRDRYIKSHPLCEECEKQGKLTPAEEVHHILPLSKGGGNEKSNLMALC
                     KSCHSRITVESGDRWGRSDL"
     gene            complement(23078..23497)
                     /locus_tag="PK733_01995"
     CDS             complement(23078..23497)
                     /locus_tag="PK733_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09350.1"
                     /translation="MKAREYLSQAYRLDQRINSKLEQVQSLNELATKATSTLTGMPRN
                     PNRATSTMADAVAKIIDLQAEINCDIDRLVDLKRDIVALVKSVDSTEFQTLLEKRYLC
                     YMSWEQIAVDMNYSIHHLYKMHNLALDICDGILHCDT"
     gene            complement(23499..23714)
                     /locus_tag="PK733_02000"
     CDS             complement(23499..23714)
                     /locus_tag="PK733_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09351.1"
                     /translation="MNPYEELANAIVLKAVKDYREALGILRYNPNDKDASISKEEIEQ
                     FFRSGWFGLLTKLDPEMLISRLHKEVA"
     gene            complement(23773..24555)
                     /locus_tag="PK733_02005"
     CDS             complement(23773..24555)
                     /locus_tag="PK733_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004055208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ09352.1"
                     /translation="MPDKRNGQVIFSYKPMPGAEQRIYDKISDITISMRSTDLLKMPE
                     LVSSEYTVRLSDEERQRYDELKSDLVLQIPDGEITAANAAALTGKLCQMANGAIYSED
                     GDTITIHDRKLDALEDIIEAAGGKPLLVAYWFKHDLARITERLQKLHVPFSKLDTADS
                     IQRWNAGELPVALIHPASAGHGLNLQSGGSCIIWFGLTWSLELYQQTNARLWRQGQNA
                     ETVVVQHIVVKGTVDERILEVLSKKDSTQAALIDAVKADLQI"
     gene            24693..24809
                     /locus_tag="PK733_02010"
                     /pseudo
     CDS             24693..24809
                     /locus_tag="PK733_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            24976..25069
                     /locus_tag="PK733_02015"
                     /pseudo
     CDS             24976..25069
                     /locus_tag="PK733_02015"
                     /inference="COORDINATES: protein motif:HMM:NF039645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25161..25290
                     /locus_tag="PK733_02020"
                     /pseudo
     CDS             25161..25290
                     /locus_tag="PK733_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587129.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3852 family protein"
     gene            complement(25394..26797)
                     /locus_tag="PK733_02025"
     CDS             complement(25394..26797)
                     /locus_tag="PK733_02025"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOJ09353.1"
                     /translation="MKTTKRLMLMNYNYYKEIEQKLEHYAKKGLVLEKMGPYLWTFKK
                     TEPQNLKYTVTYFAEGSVFNPHPTDNQQTYFDYAKAAGWDFVCEYNQMQIFSSSLENP
                     PEFETDEKEKLENIHKCMKKSFVVSQLLMLVVFALNLFLRLNSLERNPTDFLSSNIDL
                     AILLMFFSIILYLSYTLINYYMWYKKSQKSVAMGGSIIDNFNKTRRYFDIVYLIFLFS
                     LVGYMLIHLLTNTGFGIIALSVAQLPLFVLVFWGSITLLKRRKISAKANRIISISLLI
                     LTGVLYLGFIFYSISQFSFPERSNKPYTTVDWEMYEGMTREYRLYSDKIPITCEDLYG
                     NIDFEYYSYETEEEDTPLLHKDHYSQTRPPMKNAPPELEYSIYKPKFEFVKNIVVSDL
                     TKLDDWSDRKLETLDNAIFSTEEAYAFYYEEPNGKQFSGEYILVFTDKIIHIDAESPF
                     TDEQIEIVKSKLLIGQQ"
     gene            complement(26760..27113)
                     /locus_tag="PK733_02030"
     CDS             complement(26760..27113)
                     /locus_tag="PK733_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ09354.1"
                     /translation="MPREKYQTLTEQMFYILLCLQNECCGVDIMKIVSEMTQQRVTIG
                     PGTLYTLLDNFVSQGFITQTNIENRKKSYVITETGKEMLEKEYNRLHKLTDDYETFTR
                     GVVDENHKKTYVNEL"
     gene            complement(27385..28890)
                     /locus_tag="PK733_02035"
     CDS             complement(27385..28890)
                     /locus_tag="PK733_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09355.1"
                     /translation="MTIYNKFNRLNTDTSALGFDRSNKKSVYFCTPKGAKIIGWAGVD
                     GIHFCFIRDFGEMVFAISPMNIPGNYVHPLANSFSDFLRLLLACGNTSPLEQAYSWNQ
                     TEFESFLLENPITEEQLSVLNIISEKLYLQPMDNPFEYIKQLQADFDYSKIKFTEDYE
                     HFLPQEPTLPEWNVYFDGNFWGYSARQKPGKDISTQTHFNLNNKAWYIPFIYTCSKGL
                     VIDFCVQIPAERIYSFIDKWGLSPENDGGSFTEEQQMQMETENPMVVNISPEIVLNGK
                     TISYSHGSGLSWNPCFPEQNGIESDGVCKYYGLDQTSGWIIWRSAFPWQTKRKPKITS
                     LSVIIKHDPINICGSHFSISAPGEYIDFTNPTTQTLHTLTVQEYEQQEIPTDRFSDDD
                     YDFPSHCTAMSYTISPDLADEYFSVRDCSSGDRPRKKHRNSMEPQSTADCFSVGIIGG
                     AHRPTAIPFGDSEQGKLRAVCSALHFEPINTVEWKIVFHKKPCADMTVKII"
     gene            complement(29108..31153)
                     /locus_tag="PK733_02040"
     CDS             complement(29108..31153)
                     /locus_tag="PK733_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09356.1"
                     /translation="MKAYNEYMNKISVSGTLHQRLVSCVDETRPVHRPFMIKRYTAAF
                     ACLAVMLFGVFVMPQLMQNTLTPTPGENPSVSQSGKDMPTPDASSNYTLYFNKADAQS
                     AADIAIPGHFWQELTDEELKAVFPALTKTHSITATANFQSDKSGASLINIDAHAVSDT
                     GLKTYILLVPGEVVLDYGFDAETNASDVLGTPVTAGYFETNPNSKGVRNIIYFATFKL
                     SDIAYYVELDGAEVEKEALKEELTSLIGTLIKGEAADISVFHPIIPELRNDRISLDEA
                     RADADFGSYIPETLPEGFAFESATRFINQERNTLFINWTKGMGSISWRVSLLDDNDKT
                     RITSVTNTKNYDLALYPIPRADSVPEELREIVDNPIFPIDELTLDTVRARTYEVSDAG
                     DESGPRMRFSVLCRDILVELSVKGASPEAMFEILQQIAVLTDGKREVGFINTREWANY
                     TVGGDVRNYAESPEKLQELIAFPITEAASAQYSFAKLGKNGEVISGYSLQNQQLAEAI
                     IMDSLIKFSVWYGVDITTLEESYLIRQTFQGSGETHDYYAYLLDDGTAVLQRGAKGMY
                     SVLSQELYSELAESFENLIMDTWPENQYTEGVPKPDFGTMLWMRSDEEQGYFAVAYQQ
                     VSKEQIELYIQALMDDSWHTIHDFYENTTIGGLYEKGTHAISLQFAGNQFVMYLSLK"
     gene            complement(31140..31631)
                     /locus_tag="PK733_02045"
     CDS             complement(31140..31631)
                     /locus_tag="PK733_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ09357.1"
                     /translation="MAYKSYQPEDFEAFVTKHENRLYRSALAITGNISDAEDIVQEAF
                     LRAYEKAPKFESEEHEKAWLIRVTVNLCNSRLRSPWHKRRSPLLDSYPASEPEQHELL
                     EQIMALPSKYRTVIHLFYYEGYSIKDISELAGQKEATVRSHLTRARQKLKSVLKEDDY
                     ESI"
     gene            complement(31834..33906)
                     /locus_tag="PK733_02050"
     CDS             complement(31834..33906)
                     /locus_tag="PK733_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ09358.1"
                     /translation="MLDNLFLQILNMSYTASIVILFVLGARLLLKKAPKIFSYALWSV
                     VLFRLVCPFSFESLVSLLPGNPAPISDKILYAQTPQITTGITGIDNAVNATLPVPAFG
                     ASVNPIQIWTFIGEIVWLAGIAALLLYSVVSLIQLQNRLKNAVHEKENVYLPLVWVAF
                     FLSGKDMEMSCDEAVIKRLGNDVKKDYSSSLLALATGRRIVNGIPLAFGEGDTKGRIK
                     NVLNYRKPGFWVIAITVLIVVALCLGLMANPKSSTTFNGSSYRVGEILYQAPMYSFGY
                     TLDTAPQFSISSDYVLYSKQTTDQDWIMHKRLNPYKISKQELYALFNPLNNKAHEAIG
                     QTKLVYRADMNDSNQTFYLVIQLKNGDVLLALGYDSTDNRLIRWLFRLEEIGDRNGFS
                     LTDQEVGRMLGYSSAYCFSTYEMDNGLYIIGYLADGKTKHSDIGAGLFQFKDGEYKLI
                     GQTIHKGQALEQDRIVQGMLIAPSNKYYDVVLSNNENLAEIRRTSGGKVISEKVDGVN
                     PSMTVIKLPETLSDATYTFYDANGQQIDRYIPATTIKAEPFAEYPENYSPEQGEKSGM
                     FVVVHGKVFGNSLSIMEKFLLHTKQNEPSDIKILQYTEEGDPIFIDVSYDGTSYQVLT
                     DSSRDKFGGEEAKQMRIAIADYLTVFRDASMTYIYLTDSQNVTLEYAQSGSTEYGLAQ
                     PIVTYENK"
     gene            complement(33909..34271)
                     /locus_tag="PK733_02055"
     CDS             complement(33909..34271)
                     /locus_tag="PK733_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ09359.1"
                     /translation="MEQYKLGEMEQRFADLIWANEPISSRALTELCADAFSWKRTTTY
                     TMLKRLCERNIFENQSGTVVALMSKNEFQAAQGEQFINETFGGSLPQFFAAFTRRNKL
                     SEKEINELQRLIDEHKEG"
     gene            34654..34839
                     /locus_tag="PK733_02060"
     CDS             34654..34839
                     /locus_tag="PK733_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09360.1"
                     /translation="MKNRNVQKLEAITPSTLIVGIDIAKEIQWARFVDYRGLEYRALL
                     EATCKLLDNKWSNSSNG"
     gene            34760..35431
                     /locus_tag="PK733_02065"
     CDS             34760..35431
                     /locus_tag="PK733_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ09361.1"
                     /translation="MGLNTGHYWKPLANYLIINGVTVVMVNPYHTKRTKELDDNSQTK
                     NDKKDALTIAKLVRDGRYYGVYMPKDIFTELRVLTNSRISLMKRYNALKNTITAVMDE
                     YFPEITTVFKEPLKGKASIQILKSCPFPSLILELGVEGVLIEIKKAVKKTVGRKKAQQ
                     LVEAAKNSIGVDYGIVSAKLKLGLMIEELELLIRQLEQLEKAMDQALDETGFRNIILS
                     IPGIG"
BASE COUNT        10674 a         6620 c         7131 g        11078 t
ORIGIN      
        1 tatgagataa ggccttttga tttataacga ggctataaaa aatgtcctca actttctttt
       61 ttctcatcaa gagttgataa tgtcataaat ttttaaaaat ttatcgacta tatcagctct
      121 ttctccattt aatagaggct ttactataga gagagtattt attaatttat ttttatccat
      181 cccgtcaact cttaatgagg tcatattatc ttttagttgt ttatatgtat taccatataa
      241 tatacttttg tccaatgatt ttcttaaatt attcttccta taacttgcct ctgggaggta
      301 ttttgcaatt gctatcaata catcattgta catggcagtt gtagaagggt ttaccgagcg
      361 tctatattga ttattttcat ccttggaata gggctttttt ttattcgaat ctgattcatt
      421 taatttcatt atattatata tttcagctgc tgcataaata ccgttggcta agcttcccaa
      481 atattccagt ttttttgtat aactatccat aaaaacctcc atgtcctcaa tgcgcgaatt
      541 aattattgta attatatact atcaaatatt atcccgaaag atatggcttt ccaaattaac
      601 atggtagtat atatctataa ttatatttat aactatatca gcttatttta tagtatgtat
      661 gaagagtata tagtgttaca attcacgcaa tacacgcttt ttaattaaat tattgcaatt
      721 acttcaatat aaccttataa tataatctta agagagcaat atacgtaatc tgtacttgga
      781 taacttgtac tggatacacg atttatatat ggcatgatat attataatta ataaattact
      841 gagatattaa cgagatgtga ctaataatat attgtaaagg gaagatgtat tgtgagctat
      901 aaaagttttg atgaaattaa tgaaaaaatt aaaacaggta aagcagtagt agttacagca
      961 gaacaaatga ttgacatagt ggaagagaaa gggattagac aggcagctaa agaagttgat
     1021 gttgttacaa caggtacttt cggacccatg tgttcaagtg gagcatttat taatttcggc
     1081 cattcagacc cacccataag aatgagtaaa gtgtggttga atgatgtgcc ggcatatgct
     1141 ggtgttgctg ctgtagatgc atatataggt gcaactgaat tatctgaatc aaggggtatg
     1201 gaatatggtg gcgcccatgt tattgaagat ctaattgcag gcaaagatgt gcatttatat
     1261 gcagaatctt atggaaccca ttgttatccc agaaaggaaa tatcaaccta tattaataaa
     1321 aataatataa atcaggcatt catgttcaat ccaagaaatg cctatcagaa ttataatgct
     1381 gcatcaaatt cttcggaaaa aataatgtat acctatatgg gtacccttct tccaaaatgt
     1441 gcaaatgtaa catattccac atcaggacaa ttaagtcctc ttttaaatga tcctttgtac
     1501 agaactattg gtataggtac gcgaatattt atgggaggaa cccagggtta tgtggcatgg
     1561 gaaggcactc agcacaatcc ggaacagaaa agaggagaaa atggagtacc tctggcctct
     1621 gctggaacac tatctcttat tggggatttg aagaagatgg atacaaggtt cataagggct
     1681 gctgtgtttc aacaatatgg agtaacaatg tttgtaggcg ttggaattcc aatacctatc
     1741 ctggatgaag atattgcaga aaaaactgct gtaagaaaca gggatattta tactaatata
     1801 gtagattaca gtgtgtcgtc aggaccaaaa cccgtactta agaaggtttc ctacgaagag
     1861 ctaatgagtg gaagtgttga tttaaatggt aaaattatac cttcagcacc tctatcaagt
     1921 atgaagaagg ctagggaaat tgcggtaaca ttaagagaac tgataatggc aggagaattt
     1981 ttcataaccc agcctatatc atctttacca ctgactaata ccgttaataa attagagatc
     2041 agggaggatg gaaatcgatg aaaaagatta aagtaacaat gtattttcca tcaaataaaa
     2101 ttactgctcc ggtaacatat caccttataa aagattataa ccttatgctt aatattctta
     2161 atgcagatgt aagccttaat aagataggca ccctggttgt tgatattgaa ggagaggaaa
     2221 aggatattga agccggtctg gagtttgtta aaagtcaggg agccgactat aaggtatttt
     2281 ccaaatccat tatatggcag gaagaaagct gtgttcattg tggagcgtgt acggctgtat
     2341 gtccatcagg tgcattgacc atggacaaaa cagattggaa tcttaccttt gataaggaaa
     2401 agtgcctcgt atgtgaattg tgtgtcaaag catgcccatt aaaggttatg tgtgtaagaa
     2461 acggggataa agcatagaat ttatgaaaaa aagtttttat gaaaaaaggt cttacagaag
     2521 cttatttaaa ggagaaaacc tcagtttttt caatatttgt gtttatgaga ccgatttgac
     2581 cataggtatt tcagaagcca ggattaggga aaataaccag gcagaagaca atcaagtaaa
     2641 ggatagcaag gtgaaagata gccagactaa tgacagccag actaaaggca gccaggcaaa
     2701 gaatagtgca ggcaaggaat tagtgcagga agacctgctc aaaacaactc agaatctggt
     2761 taggaagtac aggcagtata ttgaagatta tataaggata catcctgagt tcttaacaag
     2821 ccttgagcct attattccaa agtccggtgc tgctatcatt gtgaatagaa tgtgtgaagc
     2881 tgcaatgaaa gcaggagtag gacctatggc tgctgtagca ggtgctatag gtcaaatggt
     2941 tggacaggaa ctgttgaaat actatgaaga agtaattatt gaaaatggcg gagatatatt
     3001 tataaaaaca aattctgtaa gaaaagtcgg ggtttatgca ggtaaatctc cgttaagtga
     3061 gaaaatagca attaaaatcc agcctcacca tactcctgct ggagtatgta catcatcagg
     3121 aactgtcggt cattccctga gttttggaaa ggcagatgca gcggttatta tttcaagaga
     3181 tgcctttttg gcagatgcag cagcaacagc agtatgcaat agagttaaat gccctgctga
     3241 tattgaaaaa gcacttaaat ttgcatctga gatagaaggt gttgatggag cgttgataat
     3301 tatagatgat aagatgggtg cgtgggggga cattgaaatt acaggatttt agtaaagact
     3361 gaaaaattac aaataaaatg taattaacaa ttaacaacta acaactaata attaacaact
     3421 aacaattaac agctaacaat taataatgaa ttaggagcaa ttatccatgg aagatataaa
     3481 tattttcagg aaaagattat atgatgctaa acgaataatt ataaaagtag gtacatctac
     3541 tctgacttat gatacaggga aagtgaatct atccagaatt gatatgctgt ctatggtgct
     3601 atctgatctt gtaaaccagg gaagggatgt tgtgctggtt acatctggag caataggggt
     3661 tggaatggga aaattaaagc ttgcggaaaa gcccggaaca atacgtgaga aacaagctgt
     3721 agcagcggtt ggacaatgtg agctgatgca tatatatagt aaattctttt ccgaatatgg
     3781 gcatattgta ggacagattt tattaacaag ggatgttgtt gaagatgagt acagaaggaa
     3841 taacgttatt aatacctttg aaactctatt ggaaaaggga attgttccta tagtaaatga
     3901 aaatgactca gtatgtgtca aggagctgga aggcgaaaat ggtgacagca tggtttttgg
     3961 cgataatgat acattatctg ctattgtggc aaaactcata aaagccgatt tgcttataat
     4021 attatcagac attgacggat tttataattg tgatccaaga aaaaatgcct gttccaggaa
     4081 gataccggta gttgccgaaa taactcccga aattgagaaa tgtgccggtg gtgaagggac
     4141 acagcgggga accggaggaa tggttactaa agttgcggca gctaaaggtg ttacttctga
     4201 aggtatagat atggttctgg ctaatggaga ggaccctaaa attataatgg atatactgga
     4261 tggtaaagat gtaggaactc tttttgtggg caggaattaa atgaattccc tttcaatatc
     4321 aatgggaaca agtaatgggt ttgcatccag aacaacttca tctccaactt ttgcatcaat
     4381 atctgttaaa tctacaacaa tattatacat acttactctg cccagaatct gtgcactttt
     4441 atcgccgatt cttacgtatg actttttatt gaaagattta atatcattat aaacgtatct
     4501 taataaatca ataagtctaa aagtgtccct tgacttttca atgccaaagc catgcctgta
     4561 gccaacagga actatacccg ttcttacggg attgcgggtt ttaaaagtat ttgcatagcc
     4621 tatattgtgc tttccaggaa gttctttggt ttctataatg ttagatttaa ggaaacctat
     4681 tctttgaagt ccatatacat tttcaattgg aatgcgcccg agaaacgcag acccgattct
     4741 taccccatcc aggtgcatct gcggaaacct taaaaaggca gaggaattgc atatatgaag
     4801 cataccggga tcaattccgt tttgacgcat tatttcaacc gaattgataa atttcctata
     4861 ttgagagtaa atatgctccg gcttttttga aaaagagaat gacaaatggg aatatgcacc
     4921 tgtaacttta atattagaca atgtactaat aaaagtacag gcatctataa cttcatccgg
     4981 aaggaacccg aacctcccaa aacctgtatc tattttaata tgcacctggg ctgtttcatt
     5041 gagccttgcg gcagcattat ttatcgtgag tgcggatgtc atggagccta tggtggtggt
     5101 aatcctattt tttataatca tttcagcttc ttcatcatca ttggtagggg tcaggagaag
     5161 caccctgccc gaaaaaccac tttctgatat ataaacagca tctctaacat ccgacagggc
     5221 aaaaatatca ataccctttt ctgataaaaa gcgggtatat tcaagcaccc ccaagccata
     5281 accgtttcct ttaacaacag ctattatttt tgcctgtgtt aatcctttta taatctctat
     5341 gtttttatca agcttgtcct tttcaattat taacttattc attcctatta tccccctgct
     5401 gtttttcctc ttggctatct ctttcctatg tattttcgct ttggttatct ttctcctgtg
     5461 cattttgact ttggttatta cctttattct ttaatataaa tagccgtctt cttaactcac
     5521 gaaatacctt ttcacctttt tctaccaagt tgtaaatgaa cgggttaaat acgtagtcca
     5581 gttcccctat gaactctgta aatttaccgc caaatccttt tttaaagcga tatagtccat
     5641 ataatgggtt gctttcatca agattgcccg atacacctct aaaatcgtag atatcgcagc
     5701 cacactcaat agcccatttt atcatttccc actgcaaaag ataattaggc atcacatttc
     5761 tgtattcatt gctgctggca ccgtataaat accaacattt atttccgtac acaactgcaa
     5821 cggttcctgc aattatttct tcgttatagt aagcaaggta taatctaata ttgtctccca
     5881 ggcagtcaag catagtttca aaatatgatg gatctcttac aacaaattca tctcttatac
     5941 cagtttcaac cattatttta tgaaattcag gtatatcagc ttttgaacct attttcacct
     6001 gtacaccttt tttagctgat aagcgaatat tataccttac tttctgatta aatgccatta
     6061 aaacctcagc ttcggtttta cctttaatat caagcctgaa aacaaagcgg ggctggatac
     6121 cttcgaaatt ttcagatgca ttttttattt taaaacccag atctcgaact atattttcaa
     6181 attccgtatc ttctttttct atatccggat ccaatttaag cacataactt ttatatttac
     6241 gtgccagttt tttggattgt tcaatcaatt ccttaaaggt ttgacggtcg tgaatatcac
     6301 atacaggacc tctgggtgag tacattattg tacggttaat aaatggcacc tttcgtataa
     6361 gaagactcat agagccttta atgttacctt tagaattcct tactgcaaca atttcattaa
     6421 cccagtttga cttaacgccg gcccattgtg gggactgcat aaaatggcct tttggatggt
     6481 gctgcaaaaa ttctgtatat tccgtatagc tttttatctc gccatgtaat tcagaactgc
     6541 tgtttttcgt atttatatta tcaatcaaat taataacctc cccgtgaaaa ttgtctcaaa
     6601 tattcttcaa actcattata atacaaaatt gtgaacttct tatcattata tattttttgc
     6661 agtaatttgt aaaaatcatc acccattttt tgatgaatat tagccatgaa tactgcagca
     6721 tctgtatatg caagcttgta atagtcatta cgcgaaccat agtcccgcaa agtcatatcc
     6781 aaaaatctac tatcaaaagt attgccaaag ttgtcgttca ttaacttcat agaaagataa
     6841 tttaccatgc cttcatcaag gaagggttca tttatctgat tcgttccgat aatgccatag
     6901 aaccattggt gaaagacctc atggtatgca actcgctgaa ggtcttcttt gttagtaaag
     6961 catttatctg aaagaaaaac catgtttgaa aactccatac cctttaaagg atgaatatcc
     7021 gctttgacaa caaaaagttt atcatagggg taaggtccta ttttttcaac agcaaacaaa
     7081 aaggcttctc taacataatc attcgcttta ctagaattaa tataataaat atttgtattt
     7141 ttaacttttt caatatgtat atcatgttca ccatttaata taactgcaga aaaatctcta
     7201 agattttttg ctgtaaaaga aacctccctg gtatttcctg tcggattttg tataccctct
     7261 tttatcccag gagcatatat ttccatgtcc ttatcagttt taaaagtgat gtaataatta
     7321 ttcatatcat atatgtaggg gtcgccaaag ctcgttttat ccataaatac aggcgtttcc
     7381 ccagcgaata ccgctggagt tgcaagaaaa tttgcaaggt aaacgcattt gccattacat
     7441 gagcgggaaa ttgtacctgt actattatta agcaaaattt caaagtctat acttatatat
     7501 gtatttgaaa tctccctatc tgtattttgt acttgtcctg ccggattgtc ctgtctggac
     7561 ctgttaagtt gtatttcaag caccatacca tttaattctg aatttattat atcattattt
     7621 gaaaatatgt ttttaatagt agtatctgca gcattaaggg aggggatata caggtatact
     7681 ttatcaacag cactagtaca atttatgtca ttttcattac taacatggtt tatatcattt
     7741 ccctctgttc tgtccggcac tttaaaatac aaatcttcct taattataac tctgtctccc
     7801 acaaagtcta tgtaagcctc aataatattc ctgccatcaa tatgattttt ctctgactga
     7861 ctattttctg ctgcactttt tctattgcta tatattgaac tataccccgg cagtacattt
     7921 tttgtgtctg cgcttacagc atttactata gtttcatcct tcaggaaata tgaaaaaagg
     7981 taaaatacca ctgttattgc agctaattca cttattattg ctactgtaat taaagtcatt
     8041 agtttttttt tcatgctaca aacaaccttc cgaagatact ttacattatg ctttaagttt
     8101 cttatttcca tatgttagat tatagcacgc ttgttcaata agtgtcaaaa tataattaga
     8161 aaacatcgat tattgataaa tagttgtgaa ttctgtataa tgattgtatg aaattatata
     8221 taacgtaaaa aatggtttta caaggtaaca taagtgcaca aaatttttat aatatagttt
     8281 cttttgtgcg ttatttatag aacagaaggc aaaatattta tatgccggta atttcccggt
     8341 gttatggtgg tattaggtat catggaagaa aattttttta aaagagtatt taaagataga
     8401 tataatatta taataatgct tctcctgctg gcaggggctg cgataatata taaactggtt
     8461 gatctgcaga ttataaacgg aaatcagtat ttcgaagatt cgcaatacaa gcttttgatg
     8521 aataaaaaga tcatggccgc caggggaaat atatttgata gaaatggagt tcctattgca
     8581 ataaaccggg ttggttttaa tgtgcagata gtcaatgctc gtcttaaaga agctgagctt
     8641 aacagcatgc ttttggatct gtgtaaaata ctggagaaaa acggtgataa ttactataat
     8701 agttttagta agtatgtaac ttatgatcct ctggatttcg gctctgaagc caaaaaatcc
     8761 caggagcggc ttgaaaaatg gattaaagat aattatataa aaattgaaat taccggttca
     8821 attgatataa aagttttatt taaagcgtta agagaacgat atggcatcga caagaaatat
     8881 accgatgaag aagcctataa aataatggta atgagatatg aagtaagaaa ttatacatct
     8941 ctgaactcag tgcttttggc taaggatgtt tctactgaat ctgtagcgga aattgaggaa
     9001 aggcaccata tattcagagg tgtcactaca gacattgaat atatgagaga gtatataaat
     9061 gccaatttgg catctcatgt cataggatat gtcagaggta ttgatgcaga cacatataac
     9121 aagcttaaaa atgaaggata cagtataaat gatattattg gcaaaacagg gatagagtat
     9181 tccgccgaaa aggaactcag ggggacaccg ggaacaaaaa aagttgaggt agataccaga
     9241 ggaaggctta cacgcgttat tgaagaaacc ccggcgatac ctggaaataa tgttgtcctt
     9301 accctcgata tgaatcttca gaaaattgct actgggattc tagaaaaaag gattaaggaa
     9361 ataagacttc ttaatggcct gtatcacttc catgatgcat ttgcgggggc agttgtggct
     9421 attgatgtaa ataatggtga agtattggct atggcaagtt atcccgggta tgatccatct
     9481 atttttcttg ctgggcctga aaacaaggaa gctcagcagg caattatgga tctaaatgat
     9541 ccttcccaga aagagataac atcagagtat aatagggcaa tttcgggcag atatgcccct
     9601 ggttcaactt ttaagcccct tattgggatt gctgctcttg aagaaggtgt aattactcct
     9661 tcaacggtta tttatgataa gggttatgta acttatgatg gcatgagatt tacctgcatg
     9721 gactggagaa atggcaaagg tgcacatggt gctattacat tgagtcatgc tcttgctaca
     9781 tcgtgcaata tattttttca tgaagccgga gttatgaccg gcatagacaa tatagacaag
     9841 tgggccgctt attttggact tggcgtaaaa acagggattg aacttcccgg ggaaattgag
     9901 ggtatccgtt caaacaagga atataagaag caagtttcac catatatttg gggaaaggct
     9961 gatactgctt cttcctctat tggccagctt tatcatgagt ttacaccttt acagctggca
    10021 aattatgtag caacaatagc aaatgggggt ataaaataca aaccccattt aataaagtgt
    10081 attacgaaat gtgatgggaa aatattgaaa gaaactgaaa tagaatatga gcaaattcct
    10141 gtaaaggaag aaacaatgga agcggtaaaa aaagggatgg tagctgttgc taactctgta
    10201 gatggcactg ctgtgaatgt ttttaaggat ctgcccttca aagttggagc aaaaacaggt
    10261 acagcagagg catatagaga aggacagtcc aataatggag tcttcatttg ctatgcgcct
    10321 gccgagcttg ataaaactcc gcaaattgct gtagccgtag ttatagagca tggtgtatat
    10381 ggcatgtatg cggcacctat agccagggaa ataattagtg agtatttaga attgaatagt
    10441 caaagtgtgg atggcataga atctgctccg gcagttcctg tttttgtacc ataagaggat
    10501 gtgaaacata cgaaagaaaa ggacagttct ttcgtgaaaa agggatggtt ttctgttttg
    10561 ctcttaagta agtctaaaca gaaaaaccgt cccctttttt cattcttggt tttattgtgt
    10621 tgttgctgtt atcgtgctgc ttttcttgtt ggtacagcta tttctgatgc tattactgct
    10681 gctgcttttc ttgttggtac agctcttcct gatgctatta ctgctgctgc ttttcttgtt
    10741 gatacagctc ttcctgctgc tgcatctgtg taagctgctc attttctccc tgatggttga
    10801 actgttgtgg cacatccatg tattcataat attgactctg tgagtttgtt gtacttgttg
    10861 tagaacttga ggaataacca tgtacattgc agtattcccc agccggatat tcgtatttcc
    10921 aatccagagg gtagggatca ccgggtttca taggcctgaa gggtattttc ctggcaacat
    10981 aaaccctctc cataatggaa tcctgaggac aatcaggtcc tgcaagaagg ttgtggcccc
    11041 aaatatcttt tgcatcttta catactttag caattatatg tgcattacaa aagtcgttgt
    11101 ctgctggttc tgtacctttt atgaagtatt cttcaaaaga agcatcacct ctaggatcat
    11161 gtgtgcatgc atcagttgca attttcccgg aataaacaca tatcttcttt ttaacaacat
    11221 tagatggtat gggaaaatcc aaaggtgctt tattgcggtg gattttctgc attaccgcat
    11281 tccatatgag ggcagcaggg ttatcattat taggtacacc tacaataggg gtgggatggt
    11341 cataaccata ccatactgcg ccagtataat atttggaata gcccacaaac catctatcct
    11401 tatcatcatc cgttgttcct gttttacctg cggtaggtat aatttcattt ttggcattct
    11461 ttattgttcc cagctttgca gctgtaccgc ctaattctcc aggccttcta gtagagcggc
    11521 atacgtcttt cagcatatct gtcattataa aggctgttgc ttcatcatat actgcattat
    11581 atgcaggctt tttttcaagg ataatgttgc catccttatc agttactttt gtataactta
    11641 ccggttcata gtatattccc ctattggtaa aaggcacata tgctgccgcc atttgcaatg
    11701 gacttacacc cttttccagg cctcccaggg atattgaaag gttcttttga ttcctgtcaa
    11761 tacctgcttt tcttaaatat tcaagtggta tattaaggtc tttaaacatg aggtatgttt
    11821 ttgctgccgg cacgttaact gaacgcttta cagcgtagcg ggtagtcata agaccttcaa
    11881 aatatctggt gtagttttca ggataccttt ttttatcaag tccaagaaaa tacacaggcg
    11941 catcatcaaa tattgtagca gcagttatta tacgtctgtc tatagcggga ccaaaatcta
    12001 caagaggttt gaagcttgat cctgcaggtc tttcaatctg tgttgctcta tttaatatgt
    12061 tgcttgcttt tttttcaccg taacctcctc ttatagcaag tatattcccg ttgtttggat
    12121 caattacaac catacctgcc tgagggtgct gagtaacttc tttattctgg tggaaatatt
    12181 tctcatccat aaaaacatcg tccattactt tctgtatatc ggggtccatt gtggtatgga
    12241 ttaaaactcc atagctgtaa accatagtca gcgccatttg cttggacata cccttctgct
    12301 ccattaagtc gttaactact tcatttatga catagtcaac aaaataactc tgaactttta
    12361 tttctttatc tgactcatct ttttcaacga atttaagctc ttcctgaaga gcctggtcat
    12421 attcgttttg gttaataaaa cccagttcaa gcattttctt taatattatt ctttgacgtt
    12481 ttacagcatt ttcccttccg ctgtcaatgt aagtattata agggtcataa tttgtcgggc
    12541 tattggtaat tccagctaaa agtgcacatt ctgccagaga caggtcctta acatcttttc
    12601 caaaatacat tcttgatgct gattgtaccc catggcagct atttcccata tatattacat
    12661 tcatatacaa ttccaggatt tgccattttt ccagcactct ttctagtttt attgccagat
    12721 accattcctg tacttttctc tcaacagatc tttttgtctt atttgttata ttacgtacta
    12781 cctgctgggt tatggtgctc ccgccatggc ttgaagagcc tgtaacaata cctaatattg
    12841 cacttgcaat tcttctaaaa tcaataccgg gatgtttttc aaacctttca tcttcaatgg
    12901 caataaaggc ttttctcagg tattctggtg tatccttgta aaaaaccggc tccctatttt
    12961 gatttaaact tcctgtaagc atggctattt cattatcttt tgagtcataa atatatgaag
    13021 tttcaatttt tatattcaat tgctcctttg ttaaaggttc agcagttcta atgtagccca
    13081 gtatagcacc ccctagaact ccggcaccgg cacaacatat agctgtaaat attaataata
    13141 ttactttaca ggtagtaaga gcatacctgc ctgcagatag cagttttgct ttgcctgcat
    13201 gtttcctggc gccattatct atatttgcag atgcagatct attaattctt gaactattag
    13261 gaatcttttt tatccgcctg aattttttcc ttcttttcgc aggaaccctt gaatgttccg
    13321 ctctattttg gttcatacaa tcctcctcac ttcctaaaaa gctttgattt actttacttt
    13381 actcctttat gtttaatgct tttaaatcat tatttcaggg ctttgatttt tagcatagtt
    13441 cttaaaatat atttacatcc ttgctcttaa aaaatatttg catctttgct cttaaaatat
    13501 attttcgtaa tcaagctctt aaaaattatt gtttaatatt tttttatttt attgctgtga
    13561 taagtcccac atgctataaa ataatcattt caattatagc ataaaattta tcactgtaac
    13621 tattaaaatt tacacaatac tattacaaat tattgacaaa aacaagatta ataaaacaag
    13681 ctgttattgt caactccaaa aaattacaag ggtgcattta taattttcgg agaaatatat
    13741 tccagaagct gtcgttatgg tgcttagagc cttcaatatt gcttcttcta aataatttct
    13801 tcaaaataaa acatgcatcc aaactgataa cttaaactgg caaaataaaa tacatagtaa
    13861 caaaaaaact actgtattca tataatgctt ttggaacaac tattttttcg gaaaggtggc
    13921 ggtgtagttt cgatgtggat aagaaataca atgctatata gacgtttccg gtatgtgaag
    13981 aaacggcata cagcgagaat acatatatat ttacgttttg ttgctacaat aatattattc
    14041 gtgattattg taaaatatgc agaaaaaact attcttcctt atatgagcaa tatatctgaa
    14101 tacagagtaa aatctgctat aaatatatta gtaaatgaag ctgtacagaa aaccttttcc
    14161 gaaaaacaga gatatgatga actagtatgt ataaataaaa atgaaaaagg tgagatagag
    14221 gcaattacta tagatacacc caaaattaat atgctgtcgg ctaaaattgc acaggaaata
    14281 cagcagaaga ttaattcacg agatacagta ttaatcaaaa tacccattgg atcattgctt
    14341 ggagaaacta tattatataa ctcaggaccg agtatatatg ccaccgtaag gcaaaatgga
    14401 aatattgaga ctgagtttat atctgagttc ctggaagcag gcataaacca aaccaaacat
    14461 aggattgtgc tatctgtaaa aataaatgtc atagtacata caacatttat caaaaagaac
    14521 cacatcatta tcaattcaat acctattgca gaaacaatta ttgtaggtaa agttccggcc
    14581 gcttattatg ctggaggtgg gaagtgagag gtgagaagtg agaagtgaaa attggtgcat
    14641 gagaatcgag aaatgaaaaa caggaatctg aggaatcaga aatcaggaat ctgaaatcaa
    14701 aaatttggaa tctgaaattt aaaaccagga atcaggaatc tgaaatctga aatctaaaac
    14761 caggaatcag gaatctgaaa tcaggaatct gaaatctgaa atcgggagag gagaattagg
    14821 aagcagagga aacaggaact cagtttcctt tttcctgtgg aactgcttca cgactgtcct
    14881 cagaaaccag agtattgcat ttgccgttaa atatggcacc ttcgtccgta ataatactga
    14941 atgcttctat atccccgtga atactccctg aagaaaaaaa tctaatattt gacttggagt
    15001 atatatttcc tttaaccata cctgatacgt aaatagcatt tccatataaa ttacctttaa
    15061 caatagcagt ctcacctata aaaatatcat catcagattt tatatcgccg gtaactttgc
    15121 catctatcct tattgcaccc tgacaattaa tattaccttc aaatattgta ttgtctccta
    15181 tcagagaatt aaaacttcct gcagatgatg ttccagtatt ttttttctta aacatagtaa
    15241 tcctcctcac tatactattt tttgaaccca tttaatttac tggtaaatat ttattcatac
    15301 ttaataaatg caccatctat cttttatcca gatatttata tgggtctata ggattatcgt
    15361 taacccttat ttcaaaatgt agatgcgatc cggtactccg tccgctgctt cctacttttg
    15421 caataatttc acctttttca actttctgac ctttcttaac taatatcttg gatgtatgcc
    15481 cgtaaacagt tgctaatcca ttaccatggt caattataac tgccaggccg tattcattat
    15541 aagagcctgt caggattact ttaccatcac ctgcggcttt tatatttgtt ccgtaagaag
    15601 ctgcaatatc aattcccttg tgaaaggaac tgcggtagga aataggatct tttcgctgac
    15661 cgaattttga tgatatatca ccattaaccg gccattgtgt tggaaggcaa tccaaatact
    15721 tccttagcgt attctcagtt tcagttaaat ctccaagttt ctcctctttc aagttgtttg
    15781 ctttttcaag ctcggtcaat atacctctta attcttcaat atcatttaca aatgtactat
    15841 ctgagcggtc tcctgatctg ctggcaagtc cgccattaat acgtcctgac acatatttat
    15901 caacgatttc tctgtatttt tctgtaagct cagaaatact cagatttgca ttgttttctt
    15961 cggttagcaa tgtatttatt tcatcagatc ttttgcttaa aatagccttt tgctgtgcat
    16021 ttaggttata catttcattt aagccggctt tcaacagcct gttctcattt accaaacctg
    16081 taaaatacaa agatgcagat actgacaata ctgcaaatat tgtgagtgac acagcagatt
    16141 tagtatacaa attagaaact ttatatgttt ttacatcgtt tgatgagtgg gggacaattg
    16201 ttatagagaa atatttttta ttatcttcag cagccccttt tacaaatctg gaacttttac
    16261 tgagcctttt ttgtcttctt aactcaaaac cggctttgat cagcttaatt aaattgattg
    16321 gaccgaccat gctgaatttt aagggtactt tattaacatt ctttcttttt attttattta
    16381 cgcggtttct cttcatgttt atgatatttt taatgctcgg ttctgtttca ttaaccattt
    16441 tgttccatcc ttaattattt taataggttt aactacatta ataactataa ttaattttat
    16501 aactatatta actttgttaa aacgcagctt ttataatctt taataagtat taacaaatat
    16561 gtcttatttt attaacaata aaattatcaa taaactagta aaaaactatt ctgtaaaaac
    16621 tattccgtaa aaactattct gcaaataaaa attaatctat acaagaaatt attctacata
    16681 aaaaatgaaa actcctcttt tttttgataa tatattaaaa atatattaaa ataatgttac
    16741 aggtaagtta atatatattt tatcttaaaa tttaaatgta cctgtatctg ggaagatgca
    16801 ataattttga taatcaatat tggattatta aatacaaaat tgtataataa aaaatacaga
    16861 tagaaataaa gttagagata tacacaatga aatatagata ggtagaaata caagcagaga
    16921 tataggtaga gatattgatg gagatactga aatagaaagg tagatatttt attatgggaa
    16981 gaataaatgg tattgataat ataagaaata agaaaacgga tgtggtaaat aaagacatag
    17041 ctccggcaaa tgctgtagag aaaaacaaaa actatgtggt tgaaattaca gatatgaatc
    17101 atgaaggtca gggtgtagga cacattggaa gtttagcggt tttcgttgat ggaggagtta
    17161 taggagagaa tattgaatta aaagttatta aagttgcaaa gaattatgcg gtcgggaaac
    17221 tgcttaaaat acttagagta tctcctggga ggactgagcc tttttgttct atatttaaga
    17281 gttgcggagg ctgcagtctg cagcatatta attataatga tcaactgcaa tacaaaacaa
    17341 atttggtgaa ggaaacgctt aagcgtatag ggaaaattga tgaggcaata gtccatgaca
    17401 ccataggtat ggaccacccc tttaactatc gaaataaagc tcagtttccg gtgggattat
    17461 gtgacggaaa accagcaata gggttttatg ctaaaagaag ccatgagata gtgggcagtt
    17521 gtgattgcgg aatacaagat cctgtcagtg acaaaatccg ggagatattt cgaaggttta
    17581 taatcgataa tgaaatctcc atttatgacg aaactactca tcttggactt gtgaggcatt
    17641 taatggttag aaagggcttc aggactggtg aggtaatggt cgttgtggta ataaacggga
    17701 aagacctgcc ttacagaaga aagcttgtag atgatttgat aagccgggtt ccaaatataa
    17761 aaagcattgt attgaatata aatacacaga atacaaatgt gatactgggc gagcgaaata
    17821 tgaagatata cggagatgat acaattgtgg attatattgg ggattttaag ttccatatat
    17881 ctccgctgtc tttttttcag gtaaatccta ttcaaactga ggtgttatat aacaaggcta
    17941 tggaatatgc ccaacttacc ggaagggaaa cagttttcga cctattttgc ggcacaggta
    18001 caattgcatt gtttatggcg caaaaggcag gaaaggtgta tggcgttgag atagtagaag
    18061 atgccatcct ggatgccagg aaaaatgcaa aactgaatag agttgggaat gccaagttta
    18121 ttgcagggga tgttgagaaa gtgataccgg agctatatgg gcagggggta agagcagatg
    18181 ttgtagtagt tgatccgcca aggaaaggtt gtgagacatc attgcttgat actattgcag
    18241 atatgggacc agaaaggatt gtgtatgtat catgtaatcc tgctactctg gccagggatt
    18301 tgatgtatct ggaggggaaa gggtatagta cggctgaagt gcagccagtg gatatgtttc
    18361 catggacggt gcacgttgag acggtagtat tgatgtcaag ggtaaaagag taagggctcg
    18421 gaaagcctgt aaataaaggg atttggatat ttcaggctca aaactgtggt attaaaaatc
    18481 atgattttac cggttcgtgg aaacaaatca aagcggctga ttttaggtga ggcagaacgg
    18541 attagatgtc ggtgcttggt gacagagtag gttagatgtc aggggtttgc gacagagcag
    18601 attagatgtt ttttgaaaaa gcggaggttg tgagagcagg ttggatgcgg tcatgaaata
    18661 gcctactttt gcagtggtaa atcgtaattt ggcaggtggg cactatgaat ttaaaagaaa
    18721 gagcaagaca attgaaaaca gatattccag ccgtgttttt gagcttgaaa agtaaggaca
    18781 cacctctgat ggctaaaatc ttagcagtga taacggttgg ctacgccctg tcacccatag
    18841 atttaatacc ggattttatt cctatactcg gatatgttga tgacgttatt attttacctg
    18901 tattaatagc cctgacaatt aagcttattc cccaagacac ttttgaaaga ttccggaaag
    18961 aagcagaggg aatgtggcag agcggtaaac caaagaagtg gtattatgcg atacctattg
    19021 ctataatttg gctattagtg atctggctaa ttgttaaagc tatttttttc tgaatgattt
    19081 gcagatgttt cgctttgagg ggtgttttcc tatggaaata tggatgttcg ttattttaat
    19141 attgtccagc tttgttgctg ctctcatatc aggggcggcc ggatttggtg gatctttgtt
    19201 acttctgcca gttgtgactg cctatgttgg ctctgaagta gccgtacctg tactaaccat
    19261 tgcgcaactg atagggaatc tgtcacgaat ggcatttggt ctaaagcaaa tagaatggaa
    19321 gtccgtcgga tggttctcgc tggcttcact acccttggcg gcgcttggcg catttgggtt
    19381 ctctctgctt ccaaaggata ttgtaacccg gtgtatcggt gcagcactaa ttttgcttgt
    19441 attaattaag ctgatgatga agagggactt acccaagggt aaaggaactc ttttgatagg
    19501 cggtgctgtg accggcggcc tttccgggct ttgcgggagt ggaggtccga ttggtgcggc
    19561 tgtgtttttg tcgcttggac tgacacctgt tgcttacata gcaagcgaag cagcgaccgc
    19621 aatggcgatg cacattttga aaacagtaat ctacagtcag cttaccaata tgactataaa
    19681 aacgtttatg gtgggtattg cgatgggggc tgccatgttg gtcggtacct tttctgcaaa
    19741 ccgttttatt aaaaagatgg ataaaaataa atttcaaaaa tatgtggcgg tgcttctgag
    19801 tgttgttgga atctatatgg tcataactgg tgcgtgaaca aaactaatat aaaaagcaac
    19861 ttgattaatt aagagcagag tgatataatc aaatattatg ggaatgaagt tctccctgtg
    19921 ccgatttaaa agccgaaccg cttattaagc tgatgacttc tgcgattatt ttattgcgga
    19981 agtgtcagct tttttgcatt tctgcacgaa ggagaatgtg tatgcttttc agtcttgcat
    20041 tgatggttct ttgtggcctt gtattaagcg gaaccatgca aaaactaaaa ataccaggcc
    20101 ttgttggtat gcttctaaca ggtatagtat taggtccata cgctttgaac ctaatagcgc
    20161 cagaattatt gaatatttcc gctgatttaa gagaaatagc tttgattgtt attcttacaa
    20221 gagcgggact cgcacttgat ataaatgatc ttaaaaaggt gggtcgtccg gctatcctca
    20281 tgtgctttat tccggcaact tttgaaatag ccgccacaac aatatttgca ccgatgttct
    20341 ttcccatttc gcacttggaa gctgctatta tgggaactgt gctcggagcc gtatccccag
    20401 cggttatcgt ccccaaaatg ctaaagttaa tggagagtgg ttatggtaaa tcaaagagta
    20461 ttccacagct aattttagcc ggagcttcgg tagacgatat ttatgtaatt gtccttttta
    20521 cgtcatttat gggaatgtat agcgggagca gttttgatat tggaagcctt gttaaaattc
    20581 cggttgctat tgtagtgggt ttattgatcg gtattttggt tggacttgcg cttgtaaaac
    20641 tatttaagat aatacacatg cgagatactg taaagatttt aattttacta agcacttctt
    20701 tcctgctcgt tactcttgaa tctgttatca aggcttatgt gcctatgtcc ggacttctgg
    20761 ccgtgatggc attaggtggt actatcctaa agcagtatgg tattttagca gagcgtctgt
    20821 caggtaagtt ttcgaaaatc tgggtggcgg ccgaattgat gttgttcgtg ctggtaggag
    20881 caacggtaga tattagttat gcggcaaggg cgggaattgc tgcagttgca cttatattca
    20941 tcgccgtcat aattcgtgtt tgcggcgtgt ttgtttgcct tatcagaact aaattaaaca
    21001 ctaaagaaag attgttttgc gctattgcct atttgcctaa agctaccgta caagctgcaa
    21061 ttggtggctt gcctttggca gccggtgttg cagcaggcaa cactattttg gctgttgcag
    21121 tattagcgat tctgattact gctccgcttg gagcaatcgg agtagatgct tcctataaaa
    21181 aattgctcac aaggggtgac agcagtgttt agacctttac gattattaaa tgatagcaaa
    21241 actgtgtcgt taaatggaca attaaagaga ggatttcatt atgcgatcct gccgtcgcaa
    21301 accggtttat gcagatatat aataacagtt aatcatggag aattgagact gaatcacatg
    21361 gcatacaacc ctgatggcgg tacatggagc tgtcctccac aaaacaggca acaggcccac
    21421 tatgatctga aacaaggtga aaaatatgaa attcaacttg taattggtga gagttttaat
    21481 aaacctgata tagttgttat agttaataag agtatgttaa atggtgcaga gttctcttac
    21541 atctttaagt atggtggcga tgaaagtaaa atttaaaata agaaaggcac tctatgctgg
    21601 tcattgatac catttcgtgc ttcaagcgca ttatctacac cagctgcaat atctgcgctt
    21661 tgctggtgta caaaaacaaa tactgtgaat ttccacgaat tgtatccaac ctcacgaagt
    21721 acatttttca cgacgaggcg gatgtctact ttaccgctgc aggtgatttc gcccgccacg
    21781 ataatttttc ctttggtcgc catgacctcg caggcaacgc gggaagcttt atctttacgc
    21841 atgcaggcat ccaaaatatt gtcagcaatg aggtcgcaaa gtttatccgg gtgtcccatg
    21901 cagacacttt cagcagtttt ataagttatc atattttcct ccaatctatt ttcctcgcct
    21961 tgccgtgagc agacgttcca ttacatcgtc ctgcgggtta acgccgctgt attcaccggt
    22021 acagttctcc ttgacgatct ggaaaatctc catccacagc cggttggttt gattcatgta
    22081 attctggccc atagccacat aggggctttg aatggcgttg cctgttgtag gatgctttgc
    22141 caaaaagccg tattcggtga ccgcttcctc tcactgaatc caacgagcca cactcatggc
    22201 atagcgttct aataactgcg gtgatacgag aactgcacat ccgcgttcat tcagccatgt
    22261 ccatgtattt ctgtagattt ctcctgcaac cagtgccttg ccatcttttt gtatagcttc
    22321 gagcatttta tttggctcgg gcatttcaag tcctttgaga tctgctgtat ccttaaatac
    22381 catcacggtc agtttcctgc caccgggatt gccttcagcg attttgtcag tcaggggctt
    22441 ctttttcgca cctgcaccga cacgagcgcc acctcgatta gtaccgtcct ttgccatatg
    22501 ttcacctcac ttttctgggc tggggctatt ccctcgtttg aaaccgcgtt ttttaacaca
    22561 aagccccacg ccgctgtccg ctttaaaaag ttttagagat ctgaccgccc ccacctgtcg
    22621 ccgctctcga cagtgattcg agagtgacag gatttacaga gagccatgag gttgctcttc
    22681 tcgttgccgc cgcctttgga gagcgggagg atgtggtgta cctcttcggc aggagtgagc
    22741 ttaccttgct tctcgcactc ctcacacaga ggatgcgatt tgatgtagcg atcacggata
    22801 cgcttccaag cacgaccata tcgtttgttg gaagcagggt cacgttcgta ctggttgtaa
    22861 tgattgtcca ttgctttctg atgctcggca cagtattgct cgcgaacagc aagccgaccg
    22921 cagccggggt aagcacaagg acgtttaggt ttgtagggca ttggttcacc tcgctttcag
    22981 ggcataagaa aagccaccgc agatctcagc gatggctctc tacattctgt ttcctcatta
    23041 taatactatc ataagaaaca ggtatcatgt ggtatcatca ggtatcacaa tggaggattc
    23101 cgtcgcaaat atccaatgcg aggttatgca ttttatatag atggtggatg ctgtagttca
    23161 tatcaacagc aatctgctcc caactcatgt aacaaaggta gcgtttttcc agaagggtct
    23221 gaaactccgt gctgtcaacg gatttcacaa gtgcaacaat gtctcgctta aggtcaacga
    23281 gacgatctat gtcgcagttg atttccgctt gcaagtcaat aatctttgct acggcatctg
    23341 ccatcgttga agtggcacgg tttggattac gaggcatacc tgtgagcgtt gaggttgctt
    23401 ttgtagccag ctcgttcagc gattgcacct gctccagttt agagtttata cgctgatcaa
    23461 ggcggtaggc ttgggagaga tattctcttg ccttcatgtc atgccacctc cttgtgaagc
    23521 ctgctaataa gcatttcagg gtcaagctta gtcagaagtc caaaccagcc tgaacgaaag
    23581 aactgctcaa tttcttcctt actgatactg gcatccttgt cattgggatt gtatcgaaga
    23641 atgccaagag cttcccggta gtccttgacc gccttaagta cgatggcatt tgctaattct
    23701 tcataagggt tcataatctg tacctccgga tttttatttt tctcggattg gcacggattg
    23761 tcgttgattg tctcagattt gcagatcggc ctttacagca tcgatcaggg cggcttgggt
    23821 gctgtccttc ttggataata cctctagaat ccgctcgtcg acggtgcctt tcacaacaat
    23881 gtgctgcacc acaaccgttt cggcattttg cccttgtcgc cagaggcggg cattggtctg
    23941 ctgatataat tccagtgacc aggtcaaccc aaaccagatg atgcaggagc cgccgctttg
    24001 aaggtttaac ccgtgaccgg cagaagctgg gtggataagc gccacgggta gctcacccgc
    24061 gttccatctt tggatactgt cagcagtgtc cagcttagag aacggaacat ggagcttttg
    24121 tagccgctca gtgatgcggg caaggtcatg cttaaaccag taggccacaa gaagcggttt
    24181 gccaccggca gcttcgatga tatcctccag cgcgtccagc tttcggtcat ggatagtgat
    24241 ggtatcgccg tcttcggagt atatagcgcc atttgccatt tggcacagct tgccggtgag
    24301 cgctgcagcg tttgcagctg ttatttcgcc atcagggatt tgcaatacga gatcactttt
    24361 caactcatcg taacgctggc gttcctcgtc ggacagacgg acggtgtatt cgctgctgac
    24421 cagctctggc attttcagca ggtcggttga ccgcatagag atggtgatgt cggagatttt
    24481 gtcatatatc cgctgctcag ctccgggcat tggtttgtag ctgaagatga cctgcccgtt
    24541 gcgtttgtcg ggcataaagt agtcaagtcg gtagtggctg ataaaccgtt gtttgtgtta
    24601 atctaaaagt accccacaat ttcagttgaa aaatccccca ccttacaagt ataatctctc
    24661 atgtatatca aacatgaggg aggcaaaatg aggtgataaa attgaatgaa aagcaggaaa
    24721 taattcttat gcatattcgt gatggcaaat cacaaagaga aattgcaaga attacaggta
    24781 aagatcgaaa aacaataagg aaatacatta aagaatatga aggaaacaga aggacattga
    24841 tcgaacatgg aggagctgat gataaagaga taataaatgc aatagttgat aaaccaaaat
    24901 atgatgtatc tggtagaaag aagaaggcaa agaacgatat tcatctctct atgacctatt
    24961 tgactgtagc tttgcaccat acttttttga agccattcgg ctgcgctatc ctgaatattg
    25021 ccggagcatg gaacgcccag catcctggct ttccgaaaaa gtgcgctaac gatcatcaat
    25081 atttctgaca aggtggtact tctatgaaac tgaaactaat tctgcctgtt ttcctcgtgg
    25141 tattgttgct gggctgtatg ctgggtacaa catgctttga ttaccgcaag aacgggtggt
    25201 ttgagtgggc aacgctggca attctttttg cctgcctggt atttaaagct cacagctcct
    25261 ctattcatgt ggacgatttt agagatgtaa taaaagtaag agccactaaa caaatgctga
    25321 agtgtagtag cccttattta tagaaataca tcagaaatct caaatgttct tctaacaggt
    25381 aattaattaa actttattgt tgcccaatta gcaacttgct tttaactatt tcaatttgct
    25441 catctgtgaa aggtgattct gcatcaatgt gaatgatttt atcagtaaaa actaatatat
    25501 attcaccgct aaactgtttc ccattcggtt cttcgtaata gaaagcatag gcttcttcag
    25561 tggaaaaaat agcgttgtca agggtttcaa gttttctgtc tgaccagtca tcaagctttg
    25621 ttaaatcgga tacaacaata ttcttaacaa attcaaattt aggtttatat atactatatt
    25681 caagctccgg tggagcattc ttcattgggg gacgggtctg agaataatgg tctttatgta
    25741 gcagaggggt gtcttcttcc tccgtttcgt aagaatagta ttcaaaatca atatttccat
    25801 ataaatcctc acaagtaatt gggattttgt cgctataaag gcgatactct cttgtcattc
    25861 cctcatacat ctcccaatca acagtggtgt atggtttatt tgatctttct gggaaactaa
    25921 actgagagat agaataaaat ataaagccaa gatacaaaac gccggttaaa ataagaagtg
    25981 aaatggagat tattctattt gcttttgcag agattttgcg tcttttcagc aatgtaattg
    26041 agccccaaaa tacaagaaca aacagtggta actgcgccac agaaagggca attatgccaa
    26101 aaccggtgtt agttaacaga tggataagca tatacccaac aagtgaaaac aggaatataa
    26161 gatagactat atcaaaatat cttcgggttt tattaaaatt gtcaatgata gagccaccca
    26221 ttgcgacaga tttttgggat ttcttgtacc acatatagta attaattagg gtataggata
    26281 aatagagaat aatgctaaaa aacataagca agatggctaa atctatatta cttgatagaa
    26341 aatccgtagg attacgctcc aagctgttaa gtcgtaaaaa aagatttaat gcaaaaacta
    26401 caagcattaa aagctgtgat accacaaagc ttttcttcat acatttgtga atgttttcaa
    26461 gcttttcttt ctcgtccgtt tcaaattccg gagggttctc aagggaagaa ctaaatattt
    26521 gcatttgatt atactcgcaa acaaaatccc atcctgccgc ctttgcgtag tcaaaatagg
    26581 tttgctgatt gtcagttggg tgaggattaa aaaccgagcc ctcagcaaaa tatgtaacgg
    26641 tgtatttaag gttttgaggt tcagttttct taaatgtcca aagataaggt cccatttttt
    26701 ctaaaacaag tccttttttc gcatagtgct ccagcttttg ctcaatctct ttgtagtagt
    26761 tataattcat taacataagt ctttttgtgg ttttcatcta caacacccct cgtaaatgtt
    26821 tcatagtcat ccgtcagctt gtgcaggcgg ttgtattctt tttcgagcat ttcttttccg
    26881 gtctctgtga taacatagct cttttttcga ttttctatgt tagtttgagt gatgaaacct
    26941 tgagatacaa agttgtcaag cagtgtatat agcgttccgg ggcctatcgt cactcgttgc
    27001 tgcgtcatct ccgatactat cttcataata tccacaccgc agcactcatt ttgcaggcat
    27061 agcaatatat agaacatctg ctctgtcaat gtttgatatt tctctcttgg catttagatc
    27121 acctccgcaa atatcgaata tcaatatttg tatttttagc gtaacatcga atatcgatat
    27181 tgtcaatagt gcagaaataa gaatcagagg ttatgatgta tcataacctc taataagctt
    27241 atatttacgg tctatttgca aggatactcc ggactatatt gggtatgcag taaaagtaag
    27301 agccactaca caaattaaaa atgtagtggc ttcacttaaa ttgaacgaac tatattttac
    27361 acattgcaaa atagttatag caattcatat tatttttaca gtcatatccg cacaaggctt
    27421 tttgtggaat acgattttcc actcaactgt atttataggc tcaaagtgca aggcagaaca
    27481 cacagcacga agctttcctt gctcactatc accaaaagga atggcagtcg gtctatgcgc
    27541 accaccaata attccgacgg aaaagcagtc ggctgtgctt tgcggctcca ttgaattacg
    27601 atgctttttg cgaggtctat cgccactgct gcaatcacga acagagaaat actcatcggc
    27661 aagatccgga gaaatggtgt agctcattgc agtacagtgg gatggaaaat cgtaatcatc
    27721 atcgctaaat ctatcggttg gaatttcctg ttgttcgtat tcctgcactg tcagcgtatg
    27781 taaagtttgg gttgtgggat tggtaaaatc aatgtattca cccggggcag atatactgaa
    27841 atgtgaaccg caaatgttga tagggtcgtg tttaattatc acactcaaag aggtgatttt
    27901 gggtttgcgt ttcgtttgcc aaggaaaagc tgaacgccag atgatccagc ccgaggtttg
    27961 gtcaagtccg tagtatttac acaccccatc gctttctatg ccattttgtt cggggaaaca
    28021 agggttccat gataaaccgc taccatgtga ataggaaatt gtttttccgt tcagtacaat
    28081 ttcaggacta atattgacaa ccatgggatt ttcggtttcc atctgcattt gctgttcttc
    28141 ggtaaagcta ccaccatcat tttctggaga aagaccccat ttgtctatga agctataaat
    28201 acgctccgca gggatttgca cacagaagtc tatgaccaac cccttactac aggtataaat
    28261 aaatgggata taccatgctt tattatttaa attaaaatgc gtttgggtag aaatatcttt
    28321 tcccggtttt tgccgagcag aatatcccca aaagttgccg tcaaaataca cgttccattc
    28381 tggaagtgtc ggctcttgtg ggagaaagtg ctcatagtct tccgtaaatt taatcttgct
    28441 gtaatcaaag tcagcttgta gttgtttgat atattcaaaa ggattgtcca ttggttgcag
    28501 atacagtttt tcagaaataa tgttcagcac tgataattgc tcttctgtaa ttggattttc
    28561 caataaaaat gattcgaatt ctgtttgatt ccagctatat gcctgttcta atggtgaggt
    28621 gttgccgcaa gcaagtaaaa gacgcagaaa atcagaaaaa ctatttgcca gcggatgaac
    28681 gtagttaccc ggtatattca tagggcttat agcaaatacc atttctccaa aatcacgtat
    28741 aaagcaaaaa tgaataccat ctacaccagc ccagccaatg attttagccc ctttgggtgt
    28801 acaaaaatac acgctttttt tattactgcg gtcaaaacca agtgccgaag tatcagtatt
    28861 taatcggttg aatttattat aaattgtcat attagtgacc ctctcaatta tgcaataact
    28921 attatgaata attttaccac aacaaactaa ttgcttcaat cggctcaatg taaacattag
    28981 aagtaaggag ttaccataaa aatataaatc cgttcaccgt ctatttgtaa ggatactctg
    29041 ggatattttg gggatataac gaagcaagag ccacaacgtc atacaaggat gatgttgcgg
    29101 ctcaacctta tttcagggaa agatacatga caaattgatt accggcaaat tgaagactga
    29161 ttgcatgggt acctttttca taaaggccgc cgatggtggt attttcgtaa aaatcatgga
    29221 tagtatgcca actatcgtcc attagtgctt ggatatatag ctctatctgt tctttgctca
    29281 cctgctgata tgcaacggcg aagtaacctt gttcctcgtc agacctcatc catagcatgg
    29341 tgccgaaatc aggctttgga acaccctcag tatattggtt ctcaggccat gtatccatta
    29401 tcagattttc aaaggattcg gcaagttcag aatagagttc ttgcgaaagt acactataca
    29461 tgccttttgc accgcgttgt aagacggctg tgccatcatc cagcaaatag gcataataat
    29521 catgggtctc acctgagcct tggaatgtct gacgaatcag gtaactttct tccaaggtgg
    29581 taatatccac accataccac actgaaaatt ttatcaaaga gtccatgatg atggcttccg
    29641 caagctgttg gttttgaaga gaatacccag aaatcacctc gccatttttc ccgagctttg
    29701 caaagctata ttgggcagag gctgcttccg taatgggaaa ggcaatcagt tcctgaagct
    29761 tttcagggct ttcggcataa tttcgcacat cccccccaac cgtataattt gcccactcac
    29821 gagtattgat aaaacctact tcacgcttac cgtctgttaa taccgcaatc tgctgcagaa
    29881 tttcaaacat tgcctctggg gaagcacctt taacgctgag ttctaccagg atatctctac
    29941 acagtacact aaagcgcatt cgcggccccg actcgtcacc cgcatccgaa acctcataag
    30001 tccgtgctcg tactgtatcg agtgtcagct catctatagg gaagattgga ttatccacga
    30061 tctcccgcaa ctcctcaggt actgaatctg cgcgaggaat ggggtaaagc gcgaggtcat
    30121 aattttttgt attggtaaca gatgtgatac gtgttttatc attatcatct aacagtgaaa
    30181 cacgccagct aatggagccc ataccctttg tccagttgat aaacagtgtg ttcctctcct
    30241 gattgataaa tcgggttgcg ctctcgaaag caaatccttc gggaagcgtt tcaggtatat
    30301 acgaaccgaa atcggcatcc gcccgggctt catcaagact tatgcgatca ttacgcaact
    30361 cgggtatgat gggatgaaag acgctgatgt cagccgcttc gcctttaatg agagtgccga
    30421 tgagggaggt taattcttcc ttgagtgctt ccttttccac ttctgcgccg tcaagctcca
    30481 cataataggc tatgtccgaa agcttgaaag tcgcaaaata gatgatattc ctaactccct
    30541 tgctattcgg attggtctca aaatacccag ctgtgactgg tgttccgagg acgtctgagg
    30601 catttgtttc agcatcaaac ccatagtcga gcacaacttc accgggcaca agcaggatat
    30661 aggtcttaag tccggtgtca gatacagcat gggcatcaat attgattaag gatgctccgc
    30721 tcttatcgct ttgaaaatta gcggtggccg taatactgtg ggtctttgtc agagcgggaa
    30781 aaactgcctt tagctcttca tcggttaatt cctgccagaa atgacctgga atagcgatat
    30841 cggctgcgga ctgagcatcg gctttattga aatacagtgt gtaattgctt gatgcatcgg
    30901 gcgtaggcat gtctttaccg gattgcgaaa cggatggatt ttcacccggt gtcggtgtta
    30961 aagtattctg cattaattga ggcattacaa agacgccaaa caacatcacg gcaagacagg
    31021 caaaggctgc tgtatagcgc ttgatcataa aaggacggtg cacgggtctt gtttcatcga
    31081 cgcaggacac aagcctttgg tgcaaagtgc ccgaaacaga tattttgttc atgtattcat
    31141 tatatgcttt cataatcatc ctcctttaaa acggacttga gcttctgacg ggcgcgagtc
    31201 aaatgacttc ttacggtagc ttccttttgc ccagccagct cagaaatatc cttaatggaa
    31261 taaccctcat aataaaacaa atggatgact gtgcgatatt tggatggtaa ggccatgatt
    31321 tgctccagta attcatgctg ctccggctct gaagccggat aagaatcaag aagcggtgac
    31381 cttcgcttgt gccaaggaga tcgcagtcgg ctgttacaga ggtttactgt tacccttatg
    31441 agccaagcct tttcatgttc ttcagattca aatttgggag ccttctcata agcacgcaga
    31501 aatgcctctt gcactatgtc ctcggcatcg gatatgtttc ctgtaatagc gagtgcagag
    31561 cgatatagcc tgttttcatg ttttgttaca aacgcctcaa aatcctccgg ctgataggac
    31621 ttatatgcca tttgcttcac ctccttcacc ctatatacgc tttagacgac aaaaaggctg
    31681 caaagtatcc cgaaaaagta tataccttat caaattaatt tagcatcgct atacaagtat
    31741 tgactgacga ttttgggtct ataacgaaac gagagccgct gcatcgatgt tgatgtgcag
    31801 cagctttatc atgcttcaat ttggattgcc attttatttg ttttcgtatg tcacgatagg
    31861 ttgcgctaag ccatattctg tactgccact ttgtgcatac tcaagggtaa cattttgact
    31921 gtctgtaaga tagatatatg tcatgctcgc atccctaaat acagtaagat aatcggcaat
    31981 agctatccgc atttgctttg cttcctcacc gccgaattta tcccgggaag aatcggttaa
    32041 gacctgatag cttgttccgt catagcttac atcaatgaaa attgggtcac cttcttcggt
    32101 atactgcagg atttttatat ctgatggttc gttttgtttt gtatgcaaaa ggaatttctc
    32161 catgatgctc agcgagttac cgaatacttt gccatgaacc acaacgaaca tcccggactt
    32221 ttctccctgc tcaggagaat agttttctgg gtattctgca aagggttctg cttttatagt
    32281 cgtggcaggg atatacctgt caatttgctg tccgtttgca tcatagaagg tatatgttgc
    32341 atcgctcaaa gtttcaggca gctttatcac agtcatactc ggattaacac catcgacttt
    32401 ttcgctgatg actttgccgc cagaggtgcg cctgatttcc gccagatttt cgttgttgct
    32461 caatacaacg tcgtaatatt tgttggaggg ggcgatgagc atcccttgaa cgatacgatc
    32521 ctgctccagt gcctgccctt tatggatggt ttgaccgatg agcttatatt ctccatcttt
    32581 aaattggaac aggccggcac cgatgtccga atgctttgtt tttccatctg caagatagcc
    32641 gatgatatac agtccgttat ccatctcata agtggagaag caatatgcgg aagaataccc
    32701 taacatacgg ccgacctctt ggtctgtcag gctaaagcca ttacgatcgc ctatttcctc
    32761 caatctaaag agccagcgaa taaggcggtt atccgtgcta tcatatccta atgctaatag
    32821 gacgtcgcca ttcttgagct ggattaccaa atagaaggtc tgattagaat cattcatatc
    32881 ggcacgataa actaattttg tttggccgat tgcttcatga gccttgttat taagtggatt
    32941 gaataatgcg tataactcct gtttgctgat tttatagggg tttaatcttt tgtgcatgat
    33001 ccaatcttgg tcggtagtct gcttactgta aagcacatag tcggatgaaa tagagaactg
    33061 aggtgcagta tccaatgtat agccaaagga gtacataggt gcttggtata gaatttctcc
    33121 aacgcgatag gatgaaccgt taaatgtcgt tgaagacttt gggtttgcca tcaatcccag
    33181 acaaagagca accactatga gcacggtgat agctataacc caaaacccag gcttcctgta
    33241 attcagcaca ttttttattc tgcccttggt gtcaccttcg ccgaaggcaa ggggaatacc
    33301 attgacgatg cgtctgccgg tagcaagtgc caacagtgaa gaagaatagt cctttttcac
    33361 atcattgcca aggcgtttga tgaccgcttc atcgcatgac atctccatgt cctttccaga
    33421 taggaaaaag gcaacccaaa ccaaaggaag gtagacattt tccttttcat ggactgcatt
    33481 tttcagccgg ttttgaagct gtatcagtga aacgacactg taaagcaaaa gtgcggcaat
    33541 accggcaagc cagacgattt ctccaataaa cgtccagatt tgtatcgggt tcacactggc
    33601 accgaaggca ggaacaggca gcgttgcgtt tacggcatta tcaatgcctg tgattcccgt
    33661 agtgatttgc ggggtctgtg catagagaat cttatcggaa atgggggcag gattaccagg
    33721 cagcaggctc accaaacttt caaaggagaa ggggcaaacc agacgaaaca gcacgacgct
    33781 ccacaaggca taagaaaaaa tcttaggcgc ttttttaagc agaagccttg cacccagcac
    33841 aaatagaatc acaatgctgg ccgtatagct catattaagt atttgcaaaa ataaattatc
    33901 cagcatgatt atccctcctt gtgctcatct attaatcgct gaagctcatt aatttccttc
    33961 tcgcttagct tattacgacg ggtaaaggca gcgaaaaact gaggcagcga accgccaaag
    34021 gtttcattga taaattgctc tccttgtgca gcctggaatt cattcttact catcagtgcg
    34081 acaacagtgc cgctctgatt ttcaaagata ttccgctcac ataaacgttt gagcattgta
    34141 tatgttgtag tgcgtttcca cgaaaatgca tcagcacaca gctctgtaag ggcacgagaa
    34201 ctgattggct cgtttgccca aatcaagtct gcgaaccgct gttccatttc cccaagcttg
    34261 tattgttcca taggaaaacc tcctgtcgaa tactattaga caaatacagt ctaacgcatt
    34321 tagacaacat tgtcaatgaa tagacaggca cactacagta ttacaggaag aaaattatag
    34381 taaggagaat ccaccatgaa cctgaataga tcgccaccca tacacaagta ttgattagca
    34441 gattattaat agtaataact tatatagaac aggttttaaa acatttaaaa cctgcttctg
    34501 ttgccttgat atagcttacg atttgtaatt cttcattagc tttttcaaat cgtaaattat
    34561 atagctttta gcttatctga gggcctataa aataacaggc tctcaatttt tttatcgaag
    34621 cattcctgta aggaatgaaa tgaactttga aaattgaaaa accgtaatgt tcaaaagcta
    34681 gaagcaataa caccaagcac actgattgtt ggaatagata ttgcaaaaga aatacagtgg
    34741 gcaaggttcg ttgattatcg tgggcttgaa tacagggcac tattggaagc cacttgcaaa
    34801 ctacttgata ataaatggag taacagtagt aatggttaat ccatatcaca caaaacggac
    34861 taaggagctt gacgataaca gccagactaa gaacgataaa aaagatgctc taaccatagc
    34921 gaagcttgtg cgtgatggaa gatattacgg agtatatatg cctaaggata tatttaccga
    34981 actaagggtg ctgacgaatt caaggataag tctgatgaaa cgttataacg cacttaaaaa
    35041 tacaataaca gcagtgatgg atgagtattt tcctgaaata acgacagtgt ttaaggaacc
    35101 tttaaaaggc aaagcatcaa tacagatatt gaaatcctgt ccatttccct cattaatact
    35161 tgaattagga gttgagggag tgcttataga gataaaaaag gcagttaaaa agacagtagg
    35221 taggaaaaaa gcgcaacagc ttgtagaagc agctaagaac tccataggag tagattatgg
    35281 aatagtatcg gcaaagttga agttagggtt aatgattgaa gaattggaat tgttgataag
    35341 acaacttgaa cagttggaaa aggcaatgga tcaagcatta gatgaaacag gattcaggaa
    35401 tataatcctc agtattccag gtataggtta ggggtagcta tcagggtaat ttccggaaag
    35461 ctgcttcaac cacagcctga aaagcaaaga aaaaaacggt ctt
//
LOCUS       DAOSDD010000012        35034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_621_length_35034_cov_5.096458, whole genome shotgun sequence.
ACCESSION   DAOSDD010000012 DAOSDD010000000
VERSION     DAOSDD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 35034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35034
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_35034_cov_5.096458"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1703
                     /gene="gltX"
                     /locus_tag="PK733_02070"
     CDS             228..1703
                     /gene="gltX"
                     /locus_tag="PK733_02070"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ09362.1"
                     /translation="MQNEVRTRFAPSPTGYMHIGNLRTALYGYLLSKKYGGKFILRIE
                     DTDQERYVEGAVDVIYKTLKLAGLKHDEGPDIGGDYGPYVQSQRKNIYMEYAKKLIES
                     GAAYYCFCDKERLEQIKGAQDGSDAVHKYDRHCLNLTKQEIEKNLAENKPFVIRQKMP
                     VQGTTSFDDVVYGTITVDNSELDDQILIKSDGLPTYNFANVVDDHLMNITHVVRGNEY
                     LSSTPKYNLLYKAFGWEIPTYVHLPLILRPTGQKLSKRAGDPSFEDLISMGYLSEAIV
                     NYVALLGWSPGSTQEIFTMDELIQVFSIDGINKAPAVFDIVKLSWMNGEYIRKLSPDK
                     FHELAVPYYKNSLKNNKIVPEKVSALLQARTDVLNSIPENIDFLNQLPDYDVSIYTHK
                     KSKTNETNSLESLKAALPVLQQLEKWDNDSIYQALMSLSEKLEIKNSQMLWPVRIAIS
                     GKSVTPGGATEIAEILGKDETIVRIQAGIEKLYRFYVLKNT"
     gene            1730..2626
                     /locus_tag="PK733_02075"
     CDS             1730..2626
                     /locus_tag="PK733_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOJ09363.1"
                     /translation="MFWTNFHNHCYFDDGTGTPEEHVEEAIQNNVEVLGFSCHASLPF
                     ETSWTMNKDTQKEYPAAIRSLQKKYEDKIQILLGLEIDYIHQSMGPYMSEYKDMGLDY
                     SIGSVHHIGSMHNIESVNHKQHNISEEYIPVDGSEKMLEDGINRVYQGNVRKAVEEYY
                     TLVQEMAILGGFDILGHLDLIKKNNKNNRFFSEDEKWYKDIVMDTITRISKSGFILEV
                     NTGGMTRKYIDTTYPSPWILKECLKLGIPITLNSDSHSPDNITAWFKNTAASLLDIGY
                     KEIHVYGENGWKPRKLTVEGLK"
     gene            complement(2685..4742)
                     /gene="ligA"
                     /locus_tag="PK733_02080"
     CDS             complement(2685..4742)
                     /gene="ligA"
                     /locus_tag="PK733_02080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOJ09364.1"
                     /translation="MDFIEKRIHELRNMINYHNHKYHVEDNPEISDYEYDMLLRELEE
                     LEKERPDLITPDSPTQRVGGKPLEGFEKVIHIIPMLSLNDVFSEGELFAFDLRVRQAI
                     GAGVSLNIGTVGADSDADIGVGTGIEYVVEKKIDGLSVSLEYENGRFIRGSTRGDGVV
                     GEDVTQNLKTVKSIPLVLKDPVPYLEVRGEVFISKKNFLKLNEEQEEAEQPLFANPRN
                     AAAGSLRQLDPRITTKRKLDIFVFNIQNIQGKSFETHSETLEYLKYQGFKISPNYKIC
                     KNINEVWDEIQKIGEARGEYSFDIDGAVVKVDSLSQRDILGSTAKTPRWAVAYKYPAE
                     QKQTVIKDIYVNVGRTGVLTPNAVLEPVRLAGSTVSRATLHNMDYIEEKDIRIGDTVW
                     IRKAGDIIPEVVEVIRERRTGDEIKFIMPDKCPVCTADVVREKGEAAFRCIDVDCPAR
                     LFRSIVHFASRDAMNIEGLGPALIESLMNKGFLKGIADLYYLYEIKDELIKMERMGKK
                     SVENLLKSIEKSKANNIDRLIFGFGIRHIGLRAAQLLSENFSSIYDLMNASEQDIMKI
                     YEFGEAMAQSLVLFFKQEKTHEIIAKLEAAGVNLLSKGKKEIIDNRFKGLTFVLTGTL
                     SSFSRNEASEIIENFGGKASGSVSKKTDYVLAGEEAGSKLDKAQQLGVKVISEDEFKK
                     MIE"
     gene            complement(4886..6136)
                     /gene="lysA"
                     /locus_tag="PK733_02085"
     CDS             complement(4886..6136)
                     /gene="lysA"
                     /locus_tag="PK733_02085"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOJ09365.1"
                     /translation="MNKYYTEQTNFYGNTAPDKLLKEFGSPLYVYNEGILRERCREMA
                     NLIPYGNFKSNFSIKANSNLELLKIARDEGLNADAMSPGEIYVLLKAGFKPDEILYIS
                     NNVSEEEMKYAIDRGILVSIDSLSQLEMLGRINYGGKVAIRFNPGVGAGHCEKVITGG
                     KKTKFGVNLDLVPEVKNILKKYNLKLAGINQHIGSLFMEGDSYAEGAKTVLETAKQFE
                     DIEFVDFGGGFGIPYRKQEGQPRLDIKELGEKLAQLVKEWTKEYGKEVRVKIEPGRYI
                     VAECGILLGTVYAVKKNYGHTYAGTDLGFNTLMRPVMYDSYHEIEVYKNGRPADGNKM
                     VVVDIVGNICESGDIMAKERNLPAISEGDIIGVMDAGAYGYSMASNYNNRLRPAEVLI
                     KQDGTAKLIRRRDTLEDLVRNFDI"
     gene            complement(7028..7579)
                     /locus_tag="PK733_02090"
     CDS             complement(7028..7579)
                     /locus_tag="PK733_02090"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOJ09366.1"
                     /translation="MECKTISLKSIEVFILIFIALISDIKTYKIKNNLILVFIIIGII
                     TNCILAGKIGLMNSLLGIIIPVAILFSLFAASMLGAGDIKVFSAIGSIMGIRFAIHTI
                     IYSFLWGGVISLAIIVIRKNRKQRLKYLYDYLKSCILTLSIVPYTNFDEKNEGDKFQF
                     AYAIVLGTVLQVIFNYSGFDLFL"
     gene            complement(7683..8075)
                     /locus_tag="PK733_02095"
     CDS             complement(7683..8075)
                     /locus_tag="PK733_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ09367.1"
                     /translation="MMMGLILFSIGFISGIIGGMGIGGGTILIPALIIFAKTEQHIAQ
                     SVNLIYFIPTAIIALVVHIKNKRIDFKTALPIIISGIVGALAGSSLAASFSSNLLRKM
                     FAFFLLIMGIYEMFRKPGDEAADVDEST"
     gene            complement(8072..8446)
                     /locus_tag="PK733_02100"
     CDS             complement(8072..8446)
                     /locus_tag="PK733_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ09368.1"
                     /translation="MENKNKNSQYMTNIAIGTVTGLVNGLFGSGGGTIAVPAMVLLLG
                     LDDHKAHATAISIILPLTIVSAFLYIRNSYVDWNITWKITLGGIAGGYLGARLLSICP
                     AHILRRIFAGFMIFAAIRMLWV"
     gene            complement(8816..10111)
                     /locus_tag="PK733_02105"
     CDS             complement(8816..10111)
                     /locus_tag="PK733_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ09369.1"
                     /translation="MNILKPEATPGNTEWFVHDRFGLFIHWGLYALPARHEWVKKYEK
                     IDNETYQKYFKHFDPDLYDPELWAKAACNAGMKYFVVTTKHHEGFCLWDTKLTDYKAP
                     NTPAGRDLLKPMVEAFRRQNMKVGFYHSLLDWHHPHYTTDICHPMSENAEYIAEDKDR
                     DLRKYTEYLHGQVKELLTDFGEVNIMWFDFSVRSTDKFPGKGREEWQSEKLIEMIRKL
                     QPGILINDRLQIDQDIKTPEQFVPREWVKINGKPVIWEACHTFSGSWGYHRDEESWKS
                     VDVLVRMLIDSVSKGGNLLLNVGPTGRGEFDERALERLNGIGRWMKRHDRSINGCTQA
                     PEEFICPQDCRYTYNPQKNRLYLHVFNWPFKHIHLDGMANRVEYAQFLNDASEIKMIT
                     SNSFSMHGGDSTSDSSTLTLELPVKKPDVTVPVIELFLK"
     gene            10461..11399
                     /locus_tag="PK733_02110"
     CDS             10461..11399
                     /locus_tag="PK733_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09370.1"
                     /translation="MSNQRDITNSNSNSNNSSNSNNNGNSDYDSNDNRIVQIKNKSGR
                     ILNNYLSNIKVGLIVAAFTHCPENWRDIDYVPDYNKLYFICDGEGWLKVGVKEYYPQP
                     GQLFFMPAGIKQSYSTISTNTFTKYWCHFTAKIGDINFFDIIKTPLFIDVENCNTLET
                     LFKKLIGHYEGRGTASPLYAQAALMEIVAYFLESIPEDKIYLSSSSSIEKLSKILEYI
                     ESNITEDITIDQLAKMASFHPNYFIRFFKKNTGTSPAHYINKVKMDKAKNLLGITDLS
                     VTSIGEAIGFSDVYHFSKIFKNYTGFSPTEYRKVFR"
     gene            complement(11592..14213)
                     /gene="clpB"
                     /locus_tag="PK733_02115"
     CDS             complement(11592..14213)
                     /gene="clpB"
                     /locus_tag="PK733_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOJ09371.1"
                     /translation="MNIDKYTEKAQQVIVASQDIALRMGHSQIDGEHIHFAMLKQEDG
                     LIPRLIGIMGENIDLVANDMQKELDIIPKIYGSSTANLYATRRFNEIFLHAEDEAAKF
                     KDEFTGVEHIYLALLKERNTPSEKIFKRYNINRERFLSALGKVRSNQRITSKNPEETY
                     EALNRFGRDLVGLAKQGKLDPVIGRDAEIRRIIRILSRRTKNNPVLIGEPGVGKTAVV
                     EGLAQRILKGDVPEGLKDKTIFALDMGSLIAGAKYRGEFEERLKAVLNEVQSSQGQII
                     MFIDELHNIVGAGKAEGAMDAGNILKPMLARGELRCIGATTLDEYRKYIEKDAALERR
                     FQPVIVEQPTVEDTISILRGLKERFEIHHGVRITDNAIIVAAVLSNRYISDRFLPDKA
                     IDLMDEAAAMIRTEIDSMPAELDEISRKIMQLEIEEQALKKENDKQSMDRLESLRKEL
                     SELKDRAATMKAQWQLEKENIEKGKVIKQQIEDVKRQIEEAERRYDLNELAILKHGKL
                     PELQKMLEEEKKRQEYPGEKQLLKEEVTEEEISEIVSKWTGIPVTKLMESEKKRLLDL
                     DNILHKRVIGQDEAVTAVADAVLRARSGLKDTRKPVGSFIFLGPTGVGKTELARALSE
                     ALFDSEENIVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDE
                     IEKAHPEVFNVLLQLLDDGRLTDSQGRTVDFKNTVIIMTSNVGSSFLLDGINEIGEIR
                     EETKNLVMNELRRDFKPEFLNRVDEIVMFKPLTKDEIIKIIDLSLTDIQKRLDDRNIK
                     LEVTAKTKQFIADEAYSPVYGARPVKRYLQKHIETPVAKLIIEGTLGEDSTAMVDSDG
                     VNIIVKHGDSSAISTVQ"
     gene            complement(14236..15213)
                     /locus_tag="PK733_02120"
     CDS             complement(14236..15213)
                     /locus_tag="PK733_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOJ09372.1"
                     /translation="MEYKDYYKILGVGKSAGQEEIKKAYRKLAKKYHPDSHPGDKKSE
                     EKFKEINEAYEVLGNEEKRKKYDTLGSGFNFQNGYDFDPSQFGYGKNARYEYRTGTGG
                     GPGAGSGFSDFFNMFFGGDSFNINDILHDFAGAGTGNKGFSYAQYPVNGNDVEAEIEI
                     TPEEGFAGTEKRVRFNISGNDKTISFKIPPGIKHGEKIKISGQGNTGINGGRNGDLYL
                     QVKFVDNTRFQVDGANLVTTVDLFPWDAALGSEIPVDTIDGRIMVKIPSGIQSDSKIR
                     VAGKGYRDTKGKRGDLFLKIRIVNPQWFANEEKELLKQLKDTYMKRSGK"
     gene            complement(15452..16834)
                     /locus_tag="PK733_02125"
     CDS             complement(15452..16834)
                     /locus_tag="PK733_02125"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ09373.1"
                     /translation="MGNLIRIKASATSHVGMIRAVNKDNFNMNGRFIYEYETDNIQVS
                     TEGSSDFFSFAVSDSMDIVDDEKNISISVTQELKKYQDKVKTDEKDLKCRTEQLFDIV
                     QENFNLIQSIYSDNKEYNSPSFACVLIKDDKASVISLGSCKVFLLREGSLKQLTVDWG
                     KTERLLKLGIITNEQASVLANRYGIPTEDSVNEIRKTDEFGIREGDTFLLCTNGLTDL
                     IELEDINEIFYSDKEPDTVVNKLIKLALKKGGDDNITALAVRIERFSGTLKNSSSPKR
                     SNMGRNLQYNANRNAPRTKKSILKLPLIGNISAGMLKIITGAIICLIIFMIIFAAVKL
                     LGVKKKGITSADNSASITNNNETLTNEKGTSNNGDTVNSNKSTEKAGNEDTENKSSQG
                     SNDDSSNQNGNNILPATYVVKKGDTLYKISKQFYNDPNKYKIIMEANNINDPSKIQIG
                     STLTIPDINN"
     gene            17233..18048
                     /locus_tag="PK733_02130"
     CDS             17233..18048
                     /locus_tag="PK733_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09374.1"
                     /translation="MKYLENAIKFIAKHFIIAIPLFISTAITALIGGTVSGGFTNKLF
                     EILPQMKGGVNYLSDPASITRFFASAAAVTGAGFLGFILTLIVTPATYGMVNKALNED
                     KSDLNEFVPQLKDNFVKYILYWVGNIVVWVILGIAAFLIVLLLALISKIAPWLGIFFG
                     VLIALAAIVVVVLIAINLVLWFPAMVIDNMDVIAALKRSYTLVKKNFWMILGINLLVG
                     IVASLASLILGILAGIPLLGPIILSVIPTASTFIIIVFHMMYYKCENNQPSAT"
     gene            complement(18169..18681)
                     /locus_tag="PK733_02135"
     CDS             complement(18169..18681)
                     /locus_tag="PK733_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOJ09375.1"
                     /translation="MSSSFKEIKPVDIPDNVFQLIGDDWMLITAGKIEDYNTMTASWG
                     GLGVLWGDPVSFCFIRPQRYTYNFIEKYENYTLSFFDKNYKKALQYCGAHSGRDVDKA
                     SAAGLTPIKDEEGIVYFNEARLVIVCKKIYYQDLVPENFIDPDIDKNYVKKDYHRMYT
                     GRITRCLIKY"
     gene            complement(18836..19690)
                     /locus_tag="PK733_02140"
     CDS             complement(18836..19690)
                     /locus_tag="PK733_02140"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ09376.1"
                     /translation="MIYGNVVLITGASSGIGKSIAQALMEKGYRVYGTSRKPQTGNNR
                     ILSKSSGNNAYVEIIQLDVRSEDSVKKAIDYVMKKEGHIDILINNAGYGIAGSVEDTT
                     AEEAFEQFDTNFFGVIRVCRNVIPIMRRNNRENGKNKNGLIINISSVAGIIPIPFQSM
                     YSASKFALESMSEALRIELEPFGIKVVIAEPGDTSTGFTDNRQFVKGANESSIYKEKF
                     FKSVNTMIKDERNGPGPETVARTILKIIDKKNPPVRITVGAGYKLVVFIKRLIPSKLL
                     HYILSKLY"
     gene            19915..21102
                     /locus_tag="PK733_02145"
     CDS             19915..21102
                     /locus_tag="PK733_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ09377.1"
                     /translation="MDIFQKCYNYTDAREVMKAGIYPYFHMLESGQDTEVIINGKRTI
                     MIGSNNYLGLTSDPRVINAAREALDKYGTGCSGSRFLNGTLKLHVELENRLAQFVNKE
                     AALTFSTGFQTNLGIISAIAGRNDYIVCDSENHASIVDGCRLSFAKVIKYEHNNMEDL
                     ERILKNIPDTNGKLIVTDGVFSMGGDICNLPEIVNLAEKYEARIMVDDAHSLGVLGEH
                     GRGTAEYFDLEDKVDIIMGTFSKSLASLGGFIAAKEEVIHYVKHISRPFIFSASIPPS
                     NAAAALEAINIIEAEPQRVKALSDIANYMREGFKKPGITIGESQTPIIPVMTWDNERT
                     FLITKLLLDEGVYVNPVVSPAVKPGQCLLRTSYTATHTTEQLDFALNAFENVFSKVKK
                     QSI"
     gene            complement(21294..22064)
                     /locus_tag="PK733_02150"
     CDS             complement(21294..22064)
                     /locus_tag="PK733_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOJ09378.1"
                     /translation="MGDLLKGKNIVIMGVRNKWSIAWGIVKSAADEGANIIFTYQGER
                     EEKEASSLASQIDNSFIYKCDISSDKDIDALFKTLKNEHGVIHGLVHAIAHANSEDIH
                     NNFINTSREGFAHALDISAYSLVAVSKRAAELMTEGGSIVTLTYMGSERVFSGYNVMG
                     VAKAALEACVRYLANDLGPLGIRINAISAGAIKTISSKAIKDFGNILGVVEQRAPLRR
                     GIDQNELGDSALFLLSDLSRAITGEIIHVDCGFNIMGI"
     gene            complement(22316..>23488)
                     /locus_tag="PK733_02155"
     CDS             complement(22316..>23488)
                     /locus_tag="PK733_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HOJ09379.1"
                     /translation="EIKGLFDKTIDTGIKHGTVTVVEEGKCFEVTTFRIDGRYLDHRR
                     PEQVEYTSSLEKDLGRRDLTINAVAYHPAQGFIDPFNGIADLQSCIIRTVGDADKRFE
                     EDALRMLRAIRFSAQLDFRIHNDVLKSIENKKALIKNISVERIRDELTKILISPAPSK
                     FILLYDTGLFELIFSELDICFNCRHEIMDKIFHKESSQIFDIIEKLENSSIVRWAVFL
                     YEVVGQVIDYEKRTAIVNKTENSDIYSDYLGKVTSSSYAVLKHLKFDKRSMDKICNIM
                     KYYNIDIRPEPKSVRKAVSIMGGELFEELIKIREAYNFTDEIRQIRDIYDNSREKGEC
                     MNLKDLAVNGEDLLEIGFEEGKKIGDVLNILLDAAIQNPEINSKSQLLSMAQKLML"
     assembly_gap    23489..23498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23499..25112)
                     /locus_tag="PK733_02160"
     CDS             complement(<23499..25112)
                     /locus_tag="PK733_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ09380.1"
                     /translation="MNKKFQRITDRIALKISSFIAYNPNDDKAVNRKNIALASYAAET
                     TNNLIGGIFFTGFLLLMSADDSYMGLVTMAGLIGNLLQVLSPLFLERFQERKKILIAG
                     RGLIYFFNIIVIGFIPYLGFPDTAKLTATIYIILLLNTISAILAPGFGVWHIKSIPEK
                     VRASFFSFYRVTSGIIVYTVILIASGLVDHFKLMGNELAGFTILRIIAIITAIIDLYF
                     QGKIKEYPNERLNSRINLVSIFVDPIKEKKYLITVAIACLWSFSASIPGPYFSIYLLK
                     DLEVKYSFLNFLNMLNIPVLIFFTPVWSRIIQRTSWFKALYISMGLYTIYYICLCFIT
                     KRTIYIYPISIIYAFIIAAGINLTFSNIPYVNIPSGNQTNFIGFYSAMNNLAALLGVL
                     LGKEFIKRTEGCIIKILGTEMQNKQYILLLTAFIMLLCTYIICILEKRAGKADKADKA
                     DNADNADNTDNADNTDNAYNTVNTDMIINTGRIINTGVIKMPEGAIKIITLLNNNGLE
                     AYMVGGCIRDSLLNSVPEDWDIATNATPCE"
     gene            complement(25171..>25400)
                     /locus_tag="PK733_02165"
     CDS             complement(25171..>25400)
                     /locus_tag="PK733_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOJ09381.1"
                     /translation="INSSEGPAFGAALLAGTGAGIYSSIQEACDTVIQVKSRQSPDMN
                     NFSRYSELHKIYKSLYSSLKDNFKDLHRWVK"
     assembly_gap    25401..25410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25411..26695)
                     /gene="xylB"
                     /locus_tag="PK733_02170"
     CDS             complement(<25411..26695)
                     /gene="xylB"
                     /locus_tag="PK733_02170"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOJ09382.1"
                     /translation="MAYIIGIDVGTSGTKTVLFDEYGGIIAGALREYQLYQPNIGWAE
                     QDPEDWWEATYLTVSEVISKSGISPDEVKAVGLSGQMHGAVLLDENNNVLRKAIIWCD
                     QRTSLECEQITDLVGSKRLVEITANPALTGFTASKIMWVKNNEPHIFEKIKKILLPKD
                     YIRYKLTGEYATEVSDASGMQLMNVPARTWSCEVLERLGIEKEWLGKLYESQEISGEI
                     TKNAARLTGLREGTPVAGGGGDQAAGAIGNGIVKKGVISSTIGTSGVVFAYTDRVTID
                     PMGRVHTFCHAIPDTWHVMGVTQGAGLSLKWFRDNFCIEEMRTAELMKIDPYVLIDQE
                     AENVLPGSGGLLYLPYLMGERTPHLDPDARGVFFGLSAKHEKPYMIRAIMEGVVFSLK
                     DCLDIMVNMGVEAKEIRASGGGGKSRLWRQLQADIF"
     gene            complement(26834..27943)
                     /locus_tag="PK733_02175"
     CDS             complement(26834..27943)
                     /locus_tag="PK733_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOJ09383.1"
                     /translation="MQKFKIRHIFAGGNTSHGFFSYFNYIINLSDANRLYILKGGPGT
                     GKSTFMKYIGSEAMNHDYDVEYFHCSSDSNSLDGVSIPSLKIAMVDGTAPHTIDPEYP
                     GVVDEIINLGEYWDKNKLKNYRDRVLRERKEIKLNFAGAYRYLGAAAVIYQDTEIINK
                     KYMDNRKLNKITFELIEDLFFSSENPKTGNEEMLKKEGTVRRLFASAITPDGVINYLD
                     SILNTSRIYGLVGDPGMGINILLEKIKEESTARGYDVECFYCGLIPNKLEHIVIPELD
                     ISLTTINKYHSTKLTIDKYFNFEDFSKKVTMEVCGNDVIENEKQIDLLIKRAVKSIIK
                     AKSIHFEMEKYYSPAMDFDRLMICKERVKNEIGLF"
     gene            complement(28034..29185)
                     /locus_tag="PK733_02180"
     CDS             complement(28034..29185)
                     /locus_tag="PK733_02180"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOJ09384.1"
                     /translation="MYFVEETKGVNYIKQFDYFLFTCVIVLTITGLVVLSSATRTMPG
                     GTGIMRTQIVSIILGVIAALVIASLDYKILKSMGLVLYFASVAMLLYVLKWGVGYEEW
                     GSRSWVLIPIIGSLRISFQPSEIAKISTVIMASIFLERIKEGQDINRDILKFIVYSAI
                     PVGLILIQPDYGMTMIFLVALVLMVYICGIKYKYIVISFAALITSSPLIWFFALNDKR
                     KKRILEFISPGSDPLGSSLQLDKAQLAIGSGRLYGSGLYKGIQTQIGSVPVKESDMIF
                     SVIGEELGFIGSVLIICLVFIIIFRCIYVARNSRDHFGTFLVIGITGMIAFQFIQNIG
                     MCLRLFPVTGLPLPFVSQGGSAMVTNYMSIGIILSVSMRRKRAMFQTNQ"
     gene            complement(29195..30256)
                     /gene="lgt"
                     /locus_tag="PK733_02185"
     CDS             complement(29195..30256)
                     /gene="lgt"
                     /locus_tag="PK733_02185"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ09385.1"
                     /translation="MGGTIEFPKLGLRFLINRIAFWIFNIPIYWYGIIIAFAFLLAVL
                     LALKHCRKYNIDPDNFIDAVLYLTPASIIGARLYYVAFSWDDFRGNLLDIINTRKGGL
                     AIYGGILGALLAVFIYTRVKKINFLELLDFGIPYLPMAQAIGRWGNFVNQEAYGVNTD
                     LPWGMTGDAIKYELALNMEKLKQMGININPEKPVHPTFLYESLWDLGVFLILIWFRKR
                     KKANGEVLFMYAILYGIGRAWIEELRTDSLMAGNIRISRLLSIIFVIAFSVLFYIKRK
                     RHVLSTAGKTGQLGTSSYGGILKELQEDESTETGEIDESTETGEIDESTETGEIDESN
                     KTNEIDKSNETNEINENKE"
     gene            complement(30276..31562)
                     /locus_tag="PK733_02190"
     CDS             complement(30276..31562)
                     /locus_tag="PK733_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ09386.1"
                     /translation="MNYETIEFKNIGETMHKYSHKSGLNAFVIPKKGYSKKYAVFAAY
                     YGSINTDFIQHNSDDVIKVPQGIAHFLEHKLFDQKEGSIMDKFSALGSEPNAYTSFNQ
                     TVYLFSCTDRFTENFNLLLSFVQNPFFTEQSVEKEKSIIKQEIDMYADNPRWRSFFNF
                     LKVLYKNNYVRNEILGTQESIDRITKDNLYMCYNNFYNMSNMVVSVVGDVETDSVFSQ
                     IENSILNSENKLIAKRIFPEEPQNINEPYIEEKMQVSIPLFQMGFKDNFNFSAGGKEL
                     LKRENAMKILLEIIIGRSSNLFISLYEDGLINNDFEFDYVAEENYAYSVINGESQNPL
                     KVKERILECIDSFIYNGISQNDYERVKKAMYGRTVRGFNSVENISGNFISAYFKGIYI
                     FDYFDIYDKINFEYVNSVMKEHFTRDRFAMSVITPF"
     gene            complement(31559..32878)
                     /locus_tag="PK733_02195"
     CDS             complement(31559..32878)
                     /locus_tag="PK733_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ09387.1"
                     /translation="MFHSFLNTEIYYIPTEKFKTNTVTFFFIDSLKKETVSKNAMIPA
                     ILRRGCKKFPEMDKISRHLEELYGASFDCGVIKKGEYQIIFFYSECISDKYITKKTSE
                     KNSKDLFEESFELLLDIVADPVIDNGSFIAESVNQEKDNLKRLIESRVNDKVKYAIEK
                     CFEEMCRDEPFGIYEYGDVTGLDEINEINLYEHYKGVMDSYPLKVYVTGDISTQKMEA
                     VKNKIISIRTGSNKNNCLVNISTDSNVPDYSNSISNTGKIREVNEVLNITQAKLSIGF
                     LTNTCSSDPDYYPLLFCDNILGGGINSKLFKYVREGAGLAYYIFSQFDKFKGMFVVSC
                     GIDAQNNDKAVELILQQVDSIKSGNISPSEYESALTSIETGINSLKDNQLQIVDYYLS
                     QVLGNTSDDFDSLLAKIRKVTTDDIKRVAGKIQPELIYLLHAGGMVK"
     gene            complement(33027..33965)
                     /locus_tag="PK733_02200"
     CDS             complement(33027..33965)
                     /locus_tag="PK733_02200"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ09388.1"
                     /translation="MKVLSTINDMSIGEDMGVGLGNFDGLHIGHMALTNILKNECKIS
                     SLQSMIYTFSKHPDNILKKDHFTPLLMTPEKKMQILEYTSVDYLFYQEFDETFSKMEP
                     VEFVKCVLVDKLGIKLAVAGFDYTFGYKGQGDARLLRQLGEKYNFKVIIVPPIKVNNE
                     VVSSTLIREYLSRGDMEKALFMLGRHYSIMGRVETGYRIGTKLGFPTANITPEEYLLL
                     PSFGVYISKTLLDGKIYNSITNLGVKPTVKTSTSKVSIETHILDFEGDIYQKPIEVFF
                     IRKIRDEKKFKSHEELVKQIKADVSTARNLFEKHIV"
     gene            complement(33977..34888)
                     /gene="truB"
                     /locus_tag="PK733_02205"
     CDS             complement(33977..34888)
                     /gene="truB"
                     /locus_tag="PK733_02205"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ09389.1"
                     /translation="MQKINGLLNVLKPPGMTSFDVIAYLRGILKVKKIGHAGTLDPGA
                     AGVLIICAGNATKAIEFVMNMDKIYRAELTLGISTDTQDSLGKVERSQQVNVSVDEIA
                     EVIKSFIGEIEQVPPMYSAVRVDGRRLYDIAREGGVIEREPRKVVIRSINIVSINQNK
                     ILFDVCCSKGTYIRTLCSDIGDCLGCGGHMSFLIRKRTGIFDIDCSNTLEDILEAVEN
                     DRIHHMLIPVDEIFKECTCICLDKKNENNFKNGSFINIEDFDFNHVDYVAGSLLRVYD
                     SNMKFVALGEIVKKDGKHLIKPKKMFQ"
BASE COUNT        10609 a         7002 c         5187 g        12216 t
ORIGIN      
        1 cttaaacata acaatccgta agctttgttc aaattttata ctcggaaaat cgtatgctgc
       61 catattttgt atcataggaa gaaaatgcac ccagggttaa gacttggcct tgacatacac
      121 aatcctgcag aatataataa aattaaattt cgatgataaa acctttcgtc cttctgcatg
      181 tacaggatgt caggtttttt tcataaatgg aaggagatat tagaaaaatg cagaatgaag
      241 ttagaacaag atttgcccca agccctacag gctatatgca tataggaaat ctaagaactg
      301 ctctttatgg atacttatta tcaaaaaaat atggtggaaa atttatactc agaattgaag
      361 ataccgacca ggaacgttat gttgaaggtg cagtcgatgt tatatataag acacttaaac
      421 ttgcgggact taagcatgat gaaggaccgg atataggtgg agattatggt ccctatgttc
      481 agagtcagcg aaaaaatata tacatggagt atgccaaaaa gcttatagaa tcgggtgcag
      541 cttactattg tttttgtgat aaggaaaggc ttgagcagat taagggagca caggatggct
      601 cagatgcagt acataaatat gaccgtcact gtttaaacct gacaaagcag gaaattgaaa
      661 aaaacctggc tgagaataaa ccttttgtaa taaggcaaaa aatgccggtt caaggaacta
      721 caagctttga tgatgttgtg tacggtacta ttacagttga caactcggag cttgatgatc
      781 aaattttgat taaatccgat ggactgccca cctataactt tgccaatgta gtagacgatc
      841 accttatgaa tattacacat gttgtaaggg gtaatgaata tctttcatct actccgaaat
      901 ataacctcct ttataaagct tttggatggg aaatccccac atatgttcat ctgcctctga
      961 tattaagacc aacagggcaa aagcttagta aaagagcagg tgatccgtca tttgaagatc
     1021 tgatttcaat gggctacctt tcagaagcaa tagtaaacta tgtggcttta ttaggctgga
     1081 gcccgggttc gacacaggaa atttttacaa tggatgaatt aatccaggta tttagtatag
     1141 atggaattaa caaagcaccg gctgtttttg atatagttaa attaagctgg atgaatggag
     1201 aatatataag aaagctttcc cctgataagt ttcatgagct tgcagttcca tattacaaga
     1261 attccctgaa aaataataag atcgtacctg aaaaagtcag tgctttactg caggcaagaa
     1321 ctgacgtttt aaactcaata ccggagaaca tagacttttt aaaccagctt ccggattatg
     1381 atgtatcaat ttatacccac aagaaatcca agactaatga aactaactct cttgagagtc
     1441 ttaaagctgc attacccgtt ttgcagcagc tggaaaagtg ggataatgat tcaatatatc
     1501 aagcattaat gagtctatca gaaaaattag aaataaagaa cagtcagatg ctttggccgg
     1561 tccgtattgc aatatcaggc aaatctgtta cacccggagg agcaacagaa attgctgaga
     1621 ttttaggtaa ggacgagacc attgttagaa tacaggcagg tattgaaaaa ttgtataggt
     1681 tttatgtatt gaaaaacaca taagagtacg aaggagaatg tagtgcatta tgttttggac
     1741 gaactttcac aaccactgtt attttgatga tggaactgga acacctgaag agcatgtaga
     1801 agaggctata cagaataatg tagaagtact gggtttttca tgccatgctt ccctgccttt
     1861 tgaaaccagc tggacaatga acaaagatac ccagaaagag tatcctgctg ccataaggtc
     1921 tcttcagaaa aaatatgagg ataaaattca aatcctgttg ggacttgaga tagattatat
     1981 acatcaatcc atggggccat atatgtctga atataaagat atgggattgg attactctat
     2041 tgggtcagtg catcatattg gctctatgca taatattgag tcggtcaatc acaaacaaca
     2101 caatatttct gaagaatata tacctgttga tggaagtgaa aagatgcttg aagatggcat
     2161 aaatagagtt tatcagggaa atgtgagaaa agctgtggag gaatattata ctcttgtcca
     2221 ggaaatggcc atcctcgggg ggtttgatat tttaggccat ctggatctta taaaaaagaa
     2281 caacaaaaac aacaggttct tttcagaaga cgaaaaatgg tataaagata ttgttatgga
     2341 taccattacc agaatttcaa aatcgggatt cattttggag gttaatacag gaggtatgac
     2401 acggaaatat attgatacaa cataccccag tccgtggatt cttaaagaat gccttaaact
     2461 tggcattcct ataacattga actcagattc acattcaccc gacaatataa cggcatggtt
     2521 taaaaacacc gcagcttctt tactcgatat aggatacaag gaaatacatg tttatggtga
     2581 aaatggctgg aaaccacgta aattaacagt tgaagggctt aagtaagaaa caaatgtacc
     2641 cttttgatca ggggacacat agtcccctaa tgaccaatca tattttactc tatcatcttt
     2701 ttgaattcat cttcacttat tactttaaca cctagttgct gagccttatc cagtttactt
     2761 cccgcctctt ccccggccag aacatagtcg gtttttttgg atacactgcc tgatgctttt
     2821 cctccaaaat tttcaataat ttctgaggct tcgtttcttg aaaaagaaga aagtgtccca
     2881 gtcaaaacaa atgtaagacc tttgaagcga ttatctataa tctctttttt acccttactc
     2941 aaaagattca ctcccgcagc ttctagttta gcgattattt catgggtctt ttcctgttta
     3001 aagaataata ccagactttg agccattgct tctccaaatt catatatctt cattatgtcc
     3061 tgttccgaag cattcattaa atcatatata gaactgaaat tttcactaag cagctgtgct
     3121 gcacgcaatc caatatgccg aatgccaaag ccgaatataa ggcggtcaat attgtttgct
     3181 ttcgactttt caatggattt tagcaaattc tcaactgatt ttttacccat tctttccatt
     3241 ttaattaatt catcttttat ttcatacagg taatacaggt cggctatacc ctttaaaaaa
     3301 cctttattca ttaaggattc aattaaagcc ggcccaagcc cttcaatatt catggcatcc
     3361 cttgaagcaa agtgaactat acttctaaaa agccttgcag gacaatctac atcaatgcat
     3421 ctgaatgccg cttctccttt ttcccggaca acatcagccg tacacaccgg gcacttatca
     3481 ggcattatga acttgatttc atcgcctgta cgcctttccc gaatcacttc aaccacttca
     3541 ggaataatat caccggcctt acgaatccag actgtatcac ctatccggat atccttctct
     3601 tcaatataat ccatattatg cagggtagca cgactcactg ttgagcctgc aagcctgaca
     3661 ggctcaagaa cagcattggg agtcaataca cctgttctac ctacattgac gtatatatcc
     3721 ttaattactg tctgtttttg ctcggcagga tatttatatg ctacagccca tcttggcgtt
     3781 ttcgctgtac tgccgagaat atccctctga gaaagagaat ctaccttaac tactgctcca
     3841 tctatatcaa atgaatattc tcccctggct tccccaatct tctgtatttc atcccatact
     3901 tcattgatat ttttgcatat tttgtagttc gggcttattt taaatccctg gtactttaaa
     3961 tattccaatg tttccgaatg agtctcaaag gatttgccct gaatattttg aatattaaat
     4021 acaaatatat caagtttcct ctttgtagta atacggggat ccaattgtct caaggaacca
     4081 gctgcagcat ttctaggatt tgcaaataaa ggctgttctg cttcttcctg ttcttcattc
     4141 agctttaaaa agttcttctt tgaaataaat acttctcccc taacttcaag gtaaggcact
     4201 ggatctttca aaactagtgg tatggacttt actgttttta gattctgggt tacatcttcc
     4261 ccaacaactc catcaccccg ggtagaaccc cttataaacc ttccattttc atattcaagg
     4321 gaaacagata gtccatcaat tttcttttca acgacatact ctattccggt tcctacgcca
     4381 atatcagcat cagaatctgc accaacagta cctatattta aagaaacacc ggcacctata
     4441 gcctgcctta ctcttaaatc aaaagcaaat agctctcctt cgctaaatac gtcgttaaga
     4501 cttaacatag gtattatatg tattaccttt tcaaatccct ctaaaggctt tccacccacc
     4561 cgctgagtag gggaatcagg agtgatcagg tcaggtcttt ccttttcaag ttcctccaat
     4621 tcccttaaca gcatatcgta ctcatagtca cttatctcag ggttgtcttc cacatgatat
     4681 ttatgattat gataattaat catgtttctt aactcatgta ttcttttttc aataaagtcc
     4741 atactacttg tattattact taacggcaca ttaatctcct tatgctctta aatatttata
     4801 tccatactta actgatacta atactaatgc tgatattaac actgatattt gctcaaatat
     4861 tactgctatt atatgctaca gtaaattata tatcaaaatt tctgaccaaa tcctccagtg
     4921 tatcccttct tcttattaat tttgctgtcc cgtcttgttt tatgagtact tctgcaggtc
     4981 ttaatctatt attataattt gatgccatag agtatccgta agctcctgca tccataactc
     5041 ctataatatc accttcacta attgcaggca gatttctctc tttagccatg atatcccctg
     5101 attcacatat gttcccaaca atatctacaa ctaccatttt gtttccatct gcaggtctgc
     5161 catttttata aacttcaatc tcatgatatg aatcatacat tacaggacgc attaatgtat
     5221 taaatccaag gtctgttcct gcataagtat gaccataatt cttcttaaca gcatatacag
     5281 tgcctaggag aattccacat tcagccacaa tgtaacgtcc tggttcaatt ttaactctta
     5341 cttcttttcc atattcttta gtccattctt taaccaattg agcaagtttt tctccaagtt
     5401 cttttatatc aagtcttggc tgaccttcct gtttgcggta aggtatacca aaaccgcctc
     5461 cgaagtcaac aaattcaata tcttcaaact gtttagcagt ttcaagtact gtttttgcac
     5521 cttcggcata tgaatctcct tccataaaaa gagagccaat gtgttggtta atgcctgcca
     5581 gctttagatt atatttcttg agtatgttct taacttcagg gacaagatca aggttaacac
     5641 caaatttggt ctttttacca cctgtaatta ccttttcaca atgcccggcc cctacgccag
     5701 ggttaaatct aatagcaact ttaccgccgt aatttattct tcccagcatc tctagttgtg
     5761 ataaggaatc tatgctcacc aaaatgcctc tgtcaatagc gtatttcatt tcttcttctg
     5821 atacattgtt tgatatatat aatatttcat caggcttgaa tcccgctttt aacaaaacat
     5881 atatttctcc gggcgacatg gcatcggcat taagcccttc atcacgagca attttcagca
     5941 gttcaaggtt tgaatttgcc ttgatggaga aatttgattt gaaattacca tatggtatca
     6001 ggttagccat ttctctgcat ctttcccgca atattccctc attatatacg taaagaggac
     6061 tgccaaattc cttaagtaat ttatccggcg cagtgtttcc ataaaaattt gtctgttcag
     6121 tatagtattt gttcatagtt tatcccccat tactaatgtt atttatgtta tttgtgttat
     6181 ttatgttatt tgtgttatat atgttatttg tgttatttat gttatttgtg ttatatatgt
     6241 tatttgtgtt atttatatta tttatattat ttacatgttt aatcacatat catgtttatt
     6301 ttgtcaacac ttagtgattt attactttta atttattatg tttcagcata atattatttt
     6361 ctctacccta taataacaca tatttctgtt ttatatcaat attctttata atttacgggt
     6421 gcatttttaa tatttcctca gttgacaaag taaatgcaac catatctacc gaaggtagat
     6481 gcattaaatc tgacaaaccc attggtttca tttagtatta caatggcaag acttatacaa
     6541 gtaaaacaaa aatgtatttt gtaggtctag ccgcgcggca atgagcgcca gggtttggga
     6601 cagagtccca aggtcttcaa ggcgctacat ttccgcatta agcaagagaa atcttagggg
     6661 gcgcacttac tctgaccgaa aaatcaggtt tttgtgaacc cctgcgcaag cttgcccttt
     6721 ttatcagcca tcataccgat tattgacaaa atactggtca tattataact aaaatcaatt
     6781 tattaccaca tattgttaat tgtttgacca actaattgct actccgttct gtttactttg
     6841 tcaaaggttg catttacttt gaccgcccac ctttttaata tttgaggtaa aataaggttc
     6901 gtaaggcctt ctatagacac actttccagc acgggataca ttttccagca cgacatattc
     6961 tcgatactat caaaaattat cctggatgac atcgctatcc aaattggcat agtagtatta
     7021 ttctatatta taaaaataaa tcaaatccac tgtaattaaa aattacttgt aatactgttc
     7081 ccaagactat tgcatatgca aattgaaact tatctccttc attcttttca tcaaaatttg
     7141 tataaggaac aatagaaagt gttaatatgc agctttttaa ataatcatat aaatacttta
     7201 acctttgttt cctatttttt ctaattacaa ttattgctaa tgaaataact cctccccata
     7261 aaaaggaata aattatcgta tgtatagcaa acctaattcc cattatggaa cctattgctg
     7321 agaatacctt aatatcacct gcgcctaaca tacttgctgc aaaaagtgaa aataatattg
     7381 ctactgggat tattattcct aagagagagt tcattaatcc tattttaccg gctaaaatac
     7441 aattagttat tatcccgatt ataataaaca ccaatataag gttattcttt attttataag
     7501 tttttatatc gctaattaat gctataaata ttagaataaa aacttctatg ctctttaagc
     7561 taatagtttt acactccatt cattattaag ttttagtttt aattagattt tactttcaca
     7621 ttgatcttta tatttattaa tataccattt aatatccata ctaccatttt atgtaattat
     7681 tgtcaagtag attcatccac atctgcagct tcatcccccg gttttctgaa catctcatat
     7741 atccccataa ttagcagaaa aaatgcgaac atttttctaa gaaggttact ggaaaaggaa
     7801 gcagcaaggc tggatcctgc cagtgctccc acaatacctg atatgattat tggcagagct
     7861 gttttaaagt caattctctt attctttata tgtacaacaa gtgctattat agcagttggg
     7921 atgaaatata ttaagttaac gctttgtgca atgtgctgtt ctgttttagc aaatataata
     7981 agtgctggta tgagtatggt accgcctcct atacccattc caccaatgat tcctgatata
     8041 aagcctatag aaaaaagaat gagtcccatc atcataccca taacatcctg attgcggcaa
     8101 atatcataaa cccggcaaat attcgtctta aaatgtgcgc gggacatata cttaagagcc
     8161 tcgctcccag gtagcctcct gctattcctc caagagtaat tttccaggtt atattccaat
     8221 ccacgtagct gtttctgata tataggaaag cgcttacaat tgttaatggc aaaatgattg
     8281 atatggcagt tgcatgagct ttatgatcgt caagccccag aagtaaaacc atggccggta
     8341 cagcaattgt tccgccaccc gacccgaaaa gcccgttcac aaggcctgta acagttccta
     8401 ttgctatatt agtcatgtat tggctattct tatttttatt ctcaatttta ttcttatttt
     8461 tattttcgtt tctatttttg ttcttcttaa tatctttttt gtctttgcta tcaatgttta
     8521 ttttactagt aatgttattt tcattcataa tgctcctcta attatcatta tcacagcctg
     8581 atatattttc tgcatcagag tgtgttttct acagcagaat atttttctac aacaagttaa
     8641 ttttctgcaa tatgattctt ccttagtttt gtcaaaaata atataaaaat acatgaaaaa
     8701 atgcccggta taatatattt ccgggcatta aggttatctt aagttatgtt aagttgtttt
     8761 aaagcattat aagtatttaa acatttgatt acttgttatt atttgcttta agtattcact
     8821 tcaggaacaa ttcaattaca ggtacagtta catcaggttt ttttaccggt aattcaagtg
     8881 tcagtgttga agaatcagat gttgagtctc caccatgcat tgagaaggaa ttcgaggtta
     8941 tcatttttat ttctgaagca tcattaagaa actgagcgta ttccaccctg tttgccatgc
     9001 cgtcaagatg gatatgctta aatggccagt tgaatacatg tagatataac ctgttctttt
     9061 gagggttata tgtataccgg caatcctgtg ggcagataaa ttcttcagga gcctgggtac
     9121 acccattaat ggaacgatca tgtcttttca tccacctgcc aataccattt aagcgttcca
     9181 acgctctttc gtcaaattct ccacgtccgg taggtcctac attaagcaga agattacctc
     9241 ccttacttac tgaatcaata agcattctaa ccagcacatc aacacttttc cagctttctt
     9301 catccctgtg gtatccccag gaaccgctaa aggtatggca tgcttcccat ataacaggtt
     9361 tcccgttaat tttaacccat tctcttggta caaactgctc tggagttttt atatcctgat
     9421 caatttgaag cctgtcattt attagaatac ccggttgaag ttttcttatc atttctataa
     9481 gtttttcgct ttgccattct tcccttcctt ttcccgggaa tttgtctgtt gagcgtactg
     9541 agaaatcaaa ccacataatg ttaacttctc caaagtccgt aagcaattct ttcacctggc
     9601 catgcaaata ttcggtatat tttcttaaat cacgatcttt gtcctctgca atatattcag
     9661 cattttcaga catgggatgg catatatctg ttgtatagtg aggatgatgc caatcaagga
     9721 gagaatgata aaatccgact ttcatatttt gtctcctaaa agcttcaacc ataggtttta
     9781 gcaggtctct gcccgccggg gtattaggtg ccttgtagtc tgtgagtttt gtatcccata
     9841 agcaaaatcc ttcatggtgc tttgttgtaa ctacgaaata tttcatacct gcattacacg
     9901 ctgcttttgc ccacaattcc gggtcataaa ggtcaggatc aaaatgcttg aaatattttt
     9961 gataggtttc attatcaatt ttttcatact tttttaccca ttcatggcga gcaggtaaag
    10021 catataagcc ccagtggata aatagtccga aacggtcatg aacgaaccat tcagtgttac
    10081 cgggtgtcgc ctctggtttt agaatgttca tacatggtta cctcctttat actttagatt
    10141 aatatatttt agtttaatac attttacacc atgtttttag actaacatgc atactgctat
    10201 tgcttcatca actggcgcta cagggattca tcacttactt tctataatgc tgagcaaaat
    10261 atcatatttg cataatagcc cttaagatga ccttgaatga tggttaatct ttctccaccc
    10321 tctaattgca ttttatattc tatatagtaa aatatgaata gatcatatta acaatatttt
    10381 gtataaaact aacataaaaa acaagttatt agatgggtaa tcaaatgaat aatcaaatga
    10441 gtgatcaaat aggtgattaa atgagtaatc aaagagatat tactaacagc aatagtaata
    10501 gtaataacag tagcaatagc aataataatg gcaatagtga ttacgatagt aatgacaata
    10561 gaatcgtaca aataaaaaat aaatccggca ggatacttaa taattactta tccaatataa
    10621 aagttgggct tattgttgct gcctttaccc actgccctga aaattggcgt gatattgatt
    10681 atgtccctga ttataacaag ttatatttca tttgtgatgg tgaaggttgg ctcaaggttg
    10741 gagttaagga gtattatccg caacccggtc agttgttttt catgcctgcg ggtattaaac
    10801 aatcttactc aaccattagc accaacactt ttacaaagta ttggtgccat tttactgcaa
    10861 aaattggaga tatcaatttt tttgatatta taaaaacccc tttattcatc gatgttgaaa
    10921 actgcaatac tctggaaacc cttttcaaaa agttaattgg acattacgaa ggacgaggta
    10981 ctgcttctcc attatatgca caagctgctt taatggaaat tgttgcttac tttttagaaa
    11041 gtatccctga agataagatt tatttgtctt cttcttcatc tattgaaaag cttagtaaaa
    11101 tacttgaata tatagaaagc aatatcactg aagatattac tatagaccaa ctggctaaaa
    11161 tggcgagctt ccatccaaac tatttcatac gtttttttaa gaaaaacaca ggaacttctc
    11221 ctgctcatta tattaacaaa gtaaaaatgg ataaagctaa aaacctttta ggaattactg
    11281 atttaagtgt tacaagtatt ggtgaagcta taggttttag tgatgtttat catttttcaa
    11341 aaattttcaa aaactataca gggttttcac ctacagaata cagaaaagtt tttaggtaaa
    11401 tttttctatt ccaagcaaga ggtactcatt tgggctattt ggcagctcac atatttgtgg
    11461 tgtttttagg gacgattctt tgttagctcg atgttgcttg agaaccgtct ttagaaccgt
    11521 cccaccttcg gaaccatccc gtccttgaaa ccattctttc tttggaacaa ccccggtgtt
    11581 tcttgtctag cttattgaac tgttgaaata gcagaactat ctccatgttt cactataata
    11641 tttaccccat ctgaatctac cattgcagtt gagtcttctc caagagttcc ttctattatt
    11701 agttttgcaa caggggtttc aatatgtttc tgcaaatatc ttttaacagg tcttgccccg
    11761 taaaccggtg aatatgcttc atcagcgata aactgttttg tcttggcagt aacttccaac
    11821 ttaatgtttc tatcatcaag tctcttttgt atatccgtta atgaaaggtc aattatctta
    11881 atgatttcat cttttgttaa aggtttgaac ataacaattt catcaactct gttgaggaac
    11941 tccggtttga agtcccttct caactcattc attactaaat tttttgtttc ttctcttatt
    12001 tcccctattt cattaattcc gtcaagcaaa aaactgctcc cgacatttga ggtcataatt
    12061 attacagtat ttttaaaatc cactgttctt ccctggctgt cagtgagtcg tccatcatcc
    12121 aaaagctgca gcagcacgtt gaaaacttca ggatgggctt tttctatttc atcaaataat
    12181 ataacactat agggttttct tcttactgct tcagtaagct gtcctccttc ttcatatccc
    12241 acatatcccg gaggtgctcc tatgagcctg gctacagaat gtttttccat atattcactc
    12301 atatcaattc taactatatt ttcctcactg tcaaataaag cttccgataa tgctcttgct
    12361 aattcagtct ttcctactcc tgtaggtcct aaaaatataa atgaccctac aggctttcta
    12421 gtatctttaa ggcccgacct tgctcttaac acagcatcag ctaccgcagt tactgcttca
    12481 tcctgcccta ttactctttt atgaagtata ttatcaagat ccagcagcct tttcttttca
    12541 ctttccataa gttttgttac aggtatgcca gtccactttg atacaatctc ggatatttct
    12601 tcttcagtta cttcttcctt caatagttgt ttttctccgg gatattcctg tctttttttc
    12661 tcttcttcca gcattttctg caattcgggt agtttaccat gtttaagtat ggcaagttca
    12721 ttaaggtcgt aacgtctttc tgcttcttct atctgcctct tcacatcctc aatctgctgc
    12781 tttatcacct tgcccttttc aatattttcc ttttcaagct gccactgcgc cttcatagtt
    12841 gctgccctat cctttaattc tgaaagctct tttctaaggg actccagcct atccatagac
    12901 tgcttgtcat tttctttttt caaagcctgt tcttctattt caagctgcat tattttcctg
    12961 cttatttcat ctagttctgc aggcatactg tctatttcag ttctaatcat tgctgctgct
    13021 tcgtccatta aatctatagc tttgtcaggt aaaaatctgt cacttatata cctgtttgac
    13081 agtactgctg ccacaataat tgcattatcg gtaattctta ctccatgatg tatttcaaac
    13141 ctttctttaa gacctcttag aattgatata gtatcttcca ctgtaggctg ttctactatt
    13201 acaggttgaa accttctttc cagagctgca tccttttcaa tgtacttgcg gtattcatca
    13261 agagttgtag cacctataca acgcaattca cctcttgcca gcatgggttt gaggatattc
    13321 ccggcatcca ttgctccttc tgctttgcct gccccaacta tattatgcaa ttcatctata
    13381 aacattatta tttggccctg tgagctctgc acctcgttaa ggacggcctt aagtctttct
    13441 tcaaattcgc cccggtattt tgcccctgca attaaggatc ccatatcaag agcaaaaata
    13501 gtcttatcct ttagcccctc aggaacatcg ccttttaata ttctttgagc cagcccttca
    13561 acaacagcag ttttacctac tccaggttca ccgattaata caggattgtt tttggttctc
    13621 ctagaaagta ttcttataat acggcgaatt tctgcatctc tcccaataac cggatcaagc
    13681 tttccctgct ttgcaagccc aactagatcc ctgccaaatc tattaagagc ttcatatgtt
    13741 tcttcaggat tttttgatgt aattcgctga ttgctcctta ccttgcctaa agctgaaagg
    13801 aatctttctc tgtttatatt ataccttttg aaaatttttt cagaaggagt atttctttct
    13861 tttaataaag ccaggtaaat atgttcaact ccggtaaatt catctttaaa ctttgcagct
    13921 tcatcttcag catgtaaaaa tatttcattg aatcttctag ttgcataaag gtttgctgtg
    13981 gagcttccat agatttttgg tattatgtca agctcttttt gcatatcatt tgcaaccaag
    14041 tctatatttt cacccatgat gccaatcagc cttggaatga gtccatcctc ctgtttcagc
    14101 atagcaaaat ggatatgttc tccatcaatc tgcgaatgac ccattctcag agcaatatct
    14161 tgtgatgcaa ctatcacctg ctgtgctttt tcagtatatt tatctatatt catatattac
    14221 acccccaaat ttgcattact ttcctgacct tttcatataa gtatctttta attgtttaag
    14281 cagttccttt tcttcatttg caaaccattg tggatttact attcttattt ttaggaacaa
    14341 gtcccctctt ttaccttttg tgtcacggta cccctttcct gctactctta ttttgctgtc
    14401 actttgtatg cccgatggaa ttttaaccat tattctgcca tcgattgtat caacaggaat
    14461 ttcactgcct aatgccgcat cccagggaaa tagatcaaca gtggtaacca gattcgcgcc
    14521 atctacttga aaacgggtat tatcaacaaa tttaacctgc aaataaaggt ctccatttct
    14581 acccccatta atacctgtat tgccctgacc ggaaattttt attttttcgc catgtttaat
    14641 accgggagga atcttaaagg aaatcgtttt atcattaccg ctaatattaa accttaccct
    14701 cttttctgtt cctgcaaaac cttcttccgg agttatctct atctcagctt ctacgtcatt
    14761 tccattaaca ggatactgtg cataggaaaa ccctttgtta cctgtgcctg cgcctgcaaa
    14821 atcatgtaaa atatcattta tattaaatga atctcctcca aaaaacatgt taaaaaaatc
    14881 actgaatccg cttcctgctc caggcccgcc accggttcct gtcctgtatt catatcttgc
    14941 atttttgcca tagccaaact gggaaggatc aaaatcataa ccattttgaa aattgaagcc
    15001 acttccaaga gtgtcatact ttttcctttt ttcctcgttt cccaggactt catatgcttc
    15061 atttatttct ttaaattttt cttcagactt tttatcacct ggatgagaat cagggtgata
    15121 ctttttagcc aattttctgt atgccttttt tatttcttct tgtcctgcac ttttacctac
    15181 tcccaatatc ttgtaataat ctttatattc cactttaagc tccattcttg caagggcgaa
    15241 ttcattttag ccacgcgtgc aatctatttt tttataatcc ctgtgcaaaa aatatgcacc
    15301 tgcgcattag tttttcataa atttattaat ttccttcaaa ctaagtcact tctaatatca
    15361 atgtatcgtt tcctgacctt gaccttcttt gaccttataa ttatataata atagaaaatt
    15421 aaaaaaaaat caatagcaaa ttaccatttt tttaattatt aatgtccggt attgttaatg
    15481 tacttccaat ctgtattttg gaaggatcat ttatattatt tgcctccata atgattttat
    15541 acttatttgg atcattatag aactgcttgc ttattttata cagtgtatca cctttcttaa
    15601 ctacatatgt agcaggaaga atattgtttc cattctgatt actactgtca tcattgctgc
    15661 cctgggatga tttattttct gtatcttcat tccctgcttt ctcagtactt ttattactat
    15721 ttacagtatc accattattt gaagtgcctt tttcgtttgt caatgtttca ttattgtttg
    15781 ttatgcttgc tgaattatca gctgaagtga tacctttttt cttaactcca agaagcttga
    15841 cagctgcaaa aataatcatg aatattatta agcatatgat agcaccggtg attattttaa
    15901 gcataccagc actgatattt ccgattaaag gaagtttcaa aatgcttttt ttagtacgag
    15961 gtgcattacg gtttgcatta tattgtaaat tacgacccat attacttcgc tttggtgacg
    16021 acgagttctt taatgttcct gaaaaccttt ctatccttac agccaatgct gtaatattat
    16081 catccccacc tttttttaac gccagtttta tcagtttgtt aactaccgta tcaggttctt
    16141 tgtccgagta aaatatttcg ttaatatctt ccaactctat caaatcagta agtccattag
    16201 tgcacagtag aaaagtatca ccttctctta tgccaaactc atccgttttt cttatttcgt
    16261 ttacactatc ttccgtagga attccatatc ggtttgctag aacactcgcc tgttcatttg
    16321 ttattattcc aagtttaagg agtctttccg ttttccccca gtcaactgtt aactgtttta
    16381 aggaaccctc tcttaataaa aatactttac agcttccaag gcttataact gaagctttgt
    16441 catcttttat gagcacacag gcgaaagatg gtgaattata ttctttatta tccgagtaaa
    16501 tactttgtat caaattaaag ttttcctgta caatatcaaa taattgttca gttctacatt
    16561 ttaaatcttt ttcatcagtc ttgactttat cctggtattt tttcagttcc tgggtaacag
    16621 aaatagaaat attcttttca tcatcaacaa tatccattga gtcgctgact gcaaaagaga
    16681 aaaaatccga actgccttct gttgatactt gaatattatc ggtttcatat tcataaatga
    16741 atcttccgtt catattgaag ttatctttat tcactgcacg aatcatgcct acatgagatg
    16801 ttgcagaagc ttttatcctt attaaatttc ccatattata actcctttct tagaaacagc
    16861 aagtactatt tatatcaatt atatcgctta atattattca atatagctca atatcactta
    16921 atgctataaa atgctactta tacccgttca gtttattttc aagcagttga taaaactatg
    16981 tctatatttg gagttaaata gtaattaaaa gtataaattc gctatgtgat ctgaattata
    17041 ttaccataat atcacaatat tctttcatat tcaattacaa ttggattact gatactgtca
    17101 ttatttgttc tttttgttca gttaacagca ttttttctga aaatattttt ttatagattt
    17161 aactcataag aaggttttct tgtaatatta atagaatatc tatactaaaa attatatttt
    17221 tggaggtgtg ttatgaagta tcttgaaaat gcaattaaat ttattgcaaa acactttatt
    17281 attgcaatac ccctctttat ttcaacagct ataactgcat taattggagg aacagtatca
    17341 ggtggattta caaataagct ttttgaaatc ctgccgcaaa tgaaaggggg tgtgaattat
    17401 ttaagcgacc ctgcaagcat tactagattt ttcgcttcag cagcagccgt cacaggagca
    17461 ggttttctgg gctttatatt aactttgatt gttactcccg ctacctatgg catggttaat
    17521 aaagcactaa acgaagataa gtctgatctt aatgaatttg tccctcagtt gaaggataac
    17581 tttgtcaagt acatacttta ttgggtaggc aacatagtag tatgggttat tttagggatt
    17641 gcagcatttt taattgtcct tttattagct ctgatatcaa agattgcacc atggttggga
    17701 attttctttg gggtcttgat tgccttggct gctattgttg ttgtagtact aattgcaatt
    17761 aatttagtgt tatggttccc tgcgatggta attgacaata tggatgttat tgcagctcta
    17821 aagagatcct acacattagt aaaaaagaat ttctggatga tacttgggat taacctttta
    17881 gtaggaatag ttgccagcct ggccagcctc atcctgggta tattagccgg gataccttta
    17941 ctcggaccga taatactaag cgttatacca acagcatcaa cattcataat aatagtattc
    18001 catatgatgt attataagtg tgaaaacaat caaccctccg caacttaatt gagatatggg
    18061 atatagattt tttaatatgc taaaatctat tctatatccc tcttctcact tctatactta
    18121 ggagtgtccc cagtaaatct atgctttaat ataatatata ctatttaatc agtatttaat
    18181 caggcacctg gtaatcctgc cggtatacat cctatgatag tcttttttta cataattttt
    18241 atctatatca ggatctataa aattctccgg taccaaatcc tgataatata tttttttaca
    18301 tacaataaca agccgtgctt cattaaaata tacaatacct tcttcatcct taattggagt
    18361 taatcctgct gctgaagctt tatccacatc tcttcctgaa tgggcaccac aatactgaag
    18421 ggctttttta taattcttat caaaaaatga aagtgtataa ttctcatatt tttcaatgaa
    18481 attgtaagta tatctttgcg gccttataaa gcaaaatgat acaggatcgc cccaaagtac
    18541 accaagccct ccccaacttg cagtcatagt attataatct tcaattttac ctgcggtaat
    18601 aagcatccaa tcatcgccaa ttaattggaa aacattatca ggtatatcca caggcttaat
    18661 ttccttaaaa ctactactca tacaattaat cctcctaata cattaattat tctcatatat
    18721 cattctctta tattttacac ataattgctt aatatttcca ctattcaggc aatttttacg
    18781 ggtatttcta tttactggtg aagctccggg aaaatgagct ctggccggtg gattttcagt
    18841 aaagctttga aagtatataa tgcagcaatt tagaaggtat taaccgctta ataaaaacta
    18901 caagtttgta ccccgccccc actgttatcc tgacaggtgg gttttttttg tctataatct
    18961 ttaaaattgt tcttgcaact gtctctgggc cgggcccgtt tctttcatcc ttaatcattg
    19021 tatttaccga cttaaaaaac ttctctttat atatggagct ttcatttgcg ccttttacaa
    19081 actgtctgtt atcggtaaat cctgtacttg tgtctcctgg ttctgctata accactttta
    19141 ttccaaatgg ctccaattca atacgtaaag cttcactcat agattccaga gcaaatttgc
    19201 tggcactgta catagattga aagggtatgg gtattatacc tgcaacagaa ctgatattga
    19261 taattaatcc atttttattc ttcccatttt ccctattgtt tcttctcatt atcgggatta
    19321 cgtttctaca tactctgatt acgccgaaga aatttgtatc aaattgttca aaagcttcct
    19381 ctgccgtagt atcttcaaca gaaccggcaa taccataccc cgcattatta atcaatatat
    19441 ctatatgtcc ctcttttttc ataacataat ctattgcttt ttttactgaa tcctctgacc
    19501 gaacatccaa ttgtatgatt tcaacatatg cattatttcc cgatgatttt gagagaatcc
    19561 tattatttcc cgtctgaggt ttcctggaag tcccatacac cctataaccc ttctccatta
    19621 acgcttgggc tattgattta ccaatacccg aggatgcccc tgttattaag actacatttc
    19681 cataaatcat ttacattccc ccttcttatt gcagctatag ctatagccat agccgtacaa
    19741 tgcaatatgt atattgtaaa gctctttttt attatatatc aataattcaa aaatacaaat
    19801 acatactttc agatggggtg catttactga atttgaagaa aaatatatac caaatactgt
    19861 ttttgtgata aaataaagtt gtttatgcgt tatttgaaag gagagaactt tacaatggac
    19921 attttccaaa aatgttataa ttatactgat gcaagagaag ttatgaaagc aggtatttat
    19981 ccatattttc atatgcttga atcaggacag gatacagaag ttataattaa cggcaaaagg
    20041 acaataatga taggttcaaa caattatctt gggctcactt cagatccaag agttataaat
    20101 gccgcacggg aggcattaga taaatatggt actggttgtt caggatcccg ttttttgaat
    20161 ggcacactca aactgcatgt agaacttgaa aaccgcttgg cgcagtttgt caataaagag
    20221 gcagcactaa cattcagtac tggttttcaa accaatctgg gaataatatc tgctattgca
    20281 ggaagaaatg attatattgt atgtgatagc gaaaatcatg caagcatagt tgatggctgc
    20341 aggttaagtt tcgctaaggt tataaaatac gaacacaaca atatggaaga tcttgagaga
    20401 atccttaaaa acatacctga cactaatggt aaattgatag taactgatgg agtattcagc
    20461 atgggaggtg atatatgcaa tcttcctgaa attgtgaatc tggcggaaaa atatgaagcc
    20521 cgtattatgg ttgatgatgc ccatagtctt ggtgttcttg gtgagcatgg aagaggcact
    20581 gctgaatact ttgatttgga agataaagta gatatcataa tgggtacttt cagcaagtct
    20641 cttgcaagtc ttggaggttt tattgcagca aaagaagaag ttatacacta tgtaaagcat
    20701 atttcaaggc cctttatatt tagtgcgtct attcctcctt caaatgccgc tgctgctctt
    20761 gaagctatta atataatcga agctgaacca caaagagtta aagcactctc agatattgca
    20821 aattacatga gagaaggttt taaaaaacca ggcataacta ttggtgaatc ccaaactccc
    20881 atcattcctg taatgacctg ggataatgaa agaacgtttt tgataacaaa actattatta
    20941 gatgaaggag tatatgtgaa ccctgttgtt tctccggcag taaaaccagg tcagtgcctg
    21001 ctgaggacaa gttatactgc aacccatact acagaacagc ttgattttgc tcttaatgca
    21061 tttgagaacg ttttcagcaa ggttaagaaa caaagtatat gaaaatcgaa aataaaaaat
    21121 aagtgataag taataagtaa tatataataa gtaatacgat aatttagaag tcccgtgtca
    21181 gaaaaaagca gaggtcagat ttaaggagta cttcaaaact cctattatta caaacctgac
    21241 ctctgttatt gttctctgaa ttaacaggat ataataaaat gagttgttta gctttaaatt
    21301 cccattatat tgaaaccaca atcaacatgg ataatctctc ctgtaatagc ccttgacaga
    21361 tcactcagca gaaaaagagc tgaatctcca agctcattct gatcaattcc ccttcttagg
    21421 ggtgcccgtt gttcaactac accaagaata tttccgaaat ctttaatcgc ctttgatgaa
    21481 attgttttga tagctccggc agaaattgca ttaattctta ttccaagagg tcccaggtcg
    21541 tttgccaggt atcttacaca tgcttccagt gcagctttgg caacccccat aacattatat
    21601 ccagaaaaca ccctctcaga acccatgtat gttaatgtta caatgcttcc gccttctgtc
    21661 ataagctctg ctgccctctt acttacagca acaagagaat aggcacttat atccagagca
    21721 tgagcaaatc cttcccttga agtatttata aaattattgt gaatatcctc actattagcg
    21781 tgcgcaatag cgtgcacaag accatgaata actccgtgtt cattttttag agttttaaac
    21841 aaagcatcaa tatctttatc tgatgaaata tcgcatttgt aaataaagga attatctatt
    21901 tgcgaagcta aggagctggc ttccttctct tctctttctc cctgatatgt aaaaattata
    21961 tttgcgcctt catcagcagc tgactttaca ataccccatg caatactcca tttatttctt
    22021 acacccataa taacaatatt tttgcctttt aaaagatcac ccatttttaa ctatcctttc
    22081 acagatgcat gtgtcctcga caatgtatat gtctccgaca caacgtaata ttatttttat
    22141 agcactaatc gtcggatgca tattatattt ttatagaaat tattagagga atcattagtg
    22201 caggctataa aaactgcaat gatatcttct ggaatatttt cttcaagaac taaaaatatg
    22261 ctcatgatca tcaggtacta tacattcata aataataaca tagaaagcct gtaatttaca
    22321 acattaattt ttgggccatg gaaagcaatt ggcttttact attaatttcc ggattttgaa
    22381 tggcagcatc aagaagaata tttaatacat caccaatctt tttaccttct tcaaacccga
    22441 tttccaaaag atcttcacca tttactgcca ggtccttgag attcatgcac tcgccttttt
    22501 ctctggagtt atcgtaaata tcccttattt gcctgatttc atcagtgaaa ttataagctt
    22561 ctctaatttt tataagctct tcaaacaatt ccccgcccat tatagatacc gctttcctga
    22621 cagatttagg ttcaggcctt atgtctatat tatagtattt cataatgttg catattttat
    22681 ccatggatct tttatcaaat ttaagatgct taaggactgc ataactgctt gatgtaactt
    22741 ttcccaaata atccgaataa atgtctgagt tttctgtttt attaacaatt gctgttcttt
    22801 tttcataatc tatgacctgt cccaccactt cgtataagaa cacagcccac ctcactattg
    22861 agctattttc caatttctct ataatatcaa aaatctgact actctcttta tgaaaaattt
    22921 tatccattat ttcatgcctg caattaaagc atatatcaag ttcagaaaag attagttcaa
    22981 aaagacctgt atcatatagt aaaataaact ttgacggtgc tggagatatt aatattttcg
    23041 ttaattcgtc cctgattctt tcaacactta tattctttat aagggctttc ttattttcaa
    23101 tactcttcaa tacatcatta tgaatccgaa aatctaattg ggcagaaaaa cgtattgctc
    23161 tcaacattct cagagcatct tcttcaaacc ttttatcagc atctcctaca gtacgaataa
    23221 tacacgactg taaatcagca ataccattaa aaggatcaat gaatccctgt gcaggatgat
    23281 aagcaacagc attaatagtt aaatccctcc tgcccagatc cttctcaaga gacgaagtgt
    23341 attctacctg ctcaggtctg cgatgatcca gatatcttcc atcaattcta aatgtggtaa
    23401 cttcaaagca cttgccttct tcaactactg taactgtacc atgctttatt cctgtgtcta
    23461 ttgttttatc aaacaaccct ttaatttcnn nnnnnnnntt cacaaggcgt tgcattagta
    23521 gctatatccc aatcttcagg aactgaattt aacaagctgt ccctgataca tcctccaacc
    23581 atgtatgcct caagcccatt attatttaat aatgtaataa tttttattgc accttcaggc
    23641 attttaatca cgcctgtatt aataattctg cctgtattaa taatcatatc agtattaacc
    23701 gtattataag cattatcagt attatcagca ttatcagtat tatcagcatt atcagcatta
    23761 tcagctttat cagctttatc agctttacct gctcttttct ccagtatgca gataatataa
    23821 gtacatagca gcattatgaa ggctgttagc aaaagaatat attgcttgtt ttgcatctct
    23881 gttcctaaga ttttaataat acacccttct gtgcgtttta taaattcttt cccaagcaaa
    23941 acacctaaaa gagcggccaa gttgttcata gctgaataaa acccaataaa atttgtctga
    24001 tttcctgaag gtatattcac atatggaata ttggaaaatg tcaggttaat tcctgctgct
    24061 attataaaag cataaattat ggaaattgga tatatatata tagtcctttt agtaataaag
    24121 caaagacaaa tatagtatat ggtatataac cccattgata tatataaggc tttaaaccat
    24181 gaagttcttt gaattatcct ggaccatacc ggtgtaaaaa atattagtac tggaatattc
    24241 aacatgttca ggaaatttaa aaatgagtat tttacttcca gatctttcag caggtatata
    24301 ctgaaatatg gaccgggtat acttgccgaa aaactccata aacaagctat agcaactgta
    24361 ataaggtatt tcttttcctt aatgggatct acaaatatac ttactaaatt aatccttgaa
    24421 tttagtcttt catttggata ttctttaatc ttgccctgga aatataaatc tattattgct
    24481 gtaattattg ctattattct tagtattgta aatcctgcaa gttcattacc cattaattta
    24541 aaatgatcta caagtccact ggcaattaaa attacagtat aaactattat tccgcttgta
    24601 actctgtaaa aagagaagaa actggcacgt actttctcag gaatgctttt tatatgccat
    24661 acaccaaaac caggagcaag tattgcactt atagtattca aaagcaatat aatatatatt
    24721 gttgcagtaa gctttgcagt atctggaaag ccaaggtaag gtataaagcc aatgacaata
    24781 atgttgaaaa aatatataag gcctctacct gcaataagga ttttctttct ttcttgaaat
    24841 ctttcaagaa agagcggaga taaaacctga agaagattac caatcaagcc agccatggta
    24901 accaatccca tatatgaatc atctgcactc attaataaaa gaaaacctgt aaaaaaaata
    24961 cctcctatga gattattggt agtttctgca gcatatgagg caagagcaat atttttacgg
    25021 ttaactgctt tatcatcgtt aggattgtat gctataaatg aagatatttt taatgcaatt
    25081 ctatcagtta ttctttgaaa tttcttattc atatagaccc catagtaact aattatttac
    25141 ctgtttttca ttatattata tcagtattaa ttacttaacc catctgtgta agtctttaaa
    25201 attatctttt aaggaactat acaaagattt gtaaatctta tgcagttcag aatacctgct
    25261 aaaattattc atatcaggac tctgtctgct ttttacctgg ataactgtat cgcatgcctc
    25321 ctgtatactg gaatagattc ccgcacctgt acctgcaagg agtgcggcac cgaacgcagg
    25381 accttcactg gaattaatag nnnnnnnnnn caaaaatgtc agcttgcaat tgtctccata
    25441 acctgctttt cccaccaccg cctgaagccc ttatttcttt agcttctact cccatattaa
    25501 ccataatatc cagacagtct ttaaggctga aaactacgcc ttccataatc gctcgtatca
    25561 tatatggctt ttcatgcttt gctgacaatc caaaaaatac tcctcttgca tctggatcaa
    25621 ggtgcggagt tctttctccc attaagtaag gaagatacaa aagacctcca cttccaggta
    25681 aaacattttc agcttcctga tcaatgagaa cgtaagggtc aattttcatc aattcagcag
    25741 tacgcatttc ctctatacag aaattatccc tgaaccattt aagtgaaagt cctgctccct
    25801 gagttacacc catcacatgc catgtatcag gtatagcatg gcagaaagta tgtactctgc
    25861 ccataggatc tatagtaacc ctgtcggtgt atgcaaatac aacccctgaa gtacctattg
    25921 ttgaagatat aacacctttt tttacgatac catttcctat ggcacctgcc gcctgatctc
    25981 caccaccacc agctacaggg gttccttctc ttagtcctgt taatcgtgca gcatttttgg
    26041 taatttcccc agatatttct tgtgattcat ataacttacc cagccactct ttttcaatac
    26101 ccagcctctc cagaacctcg caactccatg ttcttgcagg aacattcatc agctgcatac
    26161 cgctggcatc tgacacttcg gttgcatact ctcctgttaa cttatatcta atataatcct
    26221 ttggaaggag aattttttta attttttcaa aaatatgagg ctcgttgttt tttacccaca
    26281 ttatcttgga cgcggtaaag ccagtaagag ctggatttgc agtaatttct acaagccttt
    26341 tgcttccaac cagatccgtt atctgttcac attctaaaga agtcctttga tcacaccaaa
    26401 taatagcttt tctcaaaaca ttattgtttt catccaatag tactgctcca tgcatttgtc
    26461 ctgaaaggcc tacagctttt acctcatccg ggcttattcc gctttttgaa attacttcac
    26521 ttactgttaa atatgtagct tcccaccagt cttcagggtc ctgttcagcc catcctatat
    26581 taggctgata aagctgatat tcccttaatg caccggctat tattccacca tattcatcaa
    26641 acaatacggt ttttgtacct gaggtaccta catcaatacc tattatgtat gccacagaat
    26701 ccctccctaa cctcactgat attaaataac ttatatattg tcataaaaaa cagccagaat
    26761 gactaaaaaa acagtataaa cctattattg ataagtatat actgtttctg aagttatttc
    26821 aattattttt gccttaaaac agccctattt catttttaac cctttctttg caaatcatta
    26881 atctgtcaaa atccatggct ggagaataat acttttccat ctcgaaatgg atactttttg
    26941 ctttaattat tgatttaaca gcccttttta ttaaaagatc aatctgcttt tcattttcaa
    27001 taacatcatt accacatact tccattgtaa cttttttaga aaagtcttca aagttaaagt
    27061 acttatcaat agttaacttt gtgctatgat atttattaat tgttgtcaat gaaatatcaa
    27121 gttctggtat tacaatatgc tcgagcttat ttggtataag gccgcagtaa aagcattcaa
    27181 catcataacc tcttgcagtt gattcttctt ttattttttc aagcaatata tttataccca
    27241 tacctggatc tccaaccaaa ccatatattc tacttgtgtt tagtatggaa tctaaataat
    27301 ttattacacc atccggtgtt attgcactag caaataaacg tcttacagta ccctcttttt
    27361 ttagcatctc ttcatttccg gttttgggat tttcactgct gaaaaacaaa tcttctatca
    27421 attcaaaggt tattttattt agttttctat tatccatata ttttttgtta ataatctctg
    27481 tatcctggta tataacagca gctgctccta gatatctgta agctcctgca aaattgagtt
    27541 taatctcttt tctctctctt agcactctat ctctatagtt ttttaattta tttttgtccc
    27601 aatattctcc caggttaatt atttcatcta caactccagg atattccgga tcaattgtat
    27661 gaggtgcagt tccatctacc attgcaatct tcaatgaagg aattgaaact ccatcaaggc
    27721 tgttgctgtc gcttgaacag tgaaaatatt ccacatcata atcatgattc atagcttcag
    27781 aaccaatata tttcataaaa gtagacttac cggtgccggg accacccttt aatatatata
    27841 acctattagc atcacttaag tttattatgt aattaaaata gctgaaaaat ccatgtgatg
    27901 tattcccgcc ggcaaatatg tgccttattt taaatttttg catgaatcca cctccatcaa
    27961 gtaattaata cagaatagta tatgcaaaaa taaaaaataa gtgcacgggc aaacttatta
    28021 aggttatcct ggtttattga ttagtttgga acattgcccg ttttcgcctc atagatacac
    28081 taagaattat tcctatggac atatagtttg tgaccattgc acttccacct tggcttacaa
    28141 aaggcagagg gagtcctgta accggaaaaa gcctgagaca cattccaata ttttgaataa
    28201 attgaaatgc aatcattcct gttatcccga taactaaaaa tgttccaaaa tgatccctcg
    28261 aattacgtgc tacataaata cacctgaata tgataatgaa tactaaacat attattaaca
    28321 ctgacccaat aaatcccagt tcttcaccaa taacgctaaa tatcatatca gattctttta
    28381 ccggaactga tcctatctgt gtctgtatac ctttatataa accacttcca tataatcggc
    28441 ctgaacctat tgccagttga gctttgtcaa gctgcaagct tgatcctaaa ggatctgagc
    28501 cgggagatat aaattcaaga attcttttct tacgtttatc attcaaagca aaaaaccaga
    28561 ttaatggtga gctggtaatt aaagctgcga atgaaattac aatatattta tatttaattc
    28621 cgcaaatata taccattaaa accaaagcta ccaaaaatat cattgtcatc ccataatcag
    28681 gctgtattaa tataagccct acgggaatgg ctgaataaac tataaacttc aatatatctc
    28741 tgtttatatc ctgcccttct tttatacgtt caagaaaaat agatgccatt attacagtag
    28801 aaattttcgc tatttctgaa ggctgaaaag agatcctcaa acttcctatt atgggaataa
    28861 ggacccagct tcggctgccc cattcctcat atcctactcc ccatttcaga acatacaaaa
    28921 gcatggcaac acttgcaaag tataatacaa gccccattga ttttaagatt ttatagtcca
    28981 gacttgcaat taccagtgcg gctattaccc caagtataat actcacaatc tgagttctca
    29041 ttattcctgt accgccgggc atagtccttg tagcactact taaaactacc agtcccgtta
    29101 ttgtaagaac tattacacat gtaaacaaaa aataatcaaa ttgtttaatg tagtttactc
    29161 ccttggtctc ttcaacaaaa tacataaata ccctttattc tttgttttca tttatttcat
    29221 tagtttcatt gctcttatct atttcattag ttttattgct ctcatctatt tcgccagttt
    29281 cggtgctttc atctatttcg ccagtttcag tgctttcatc tatttcgcca gtttcagtgc
    29341 tttcgtcttc ctgaagctcc tttagtattc caccatagct gcttgttcca agttgccctg
    29401 tttttccagc tgtagataga acatgtcttt tgcgttttat ataaaataaa acagaaaaag
    29461 ctattacaaa aattatcgat aaaagcctcg atattcttat atttccagcc ataaggctgt
    29521 ctgttctgag ttcctctatc catgctcttc caataccata caatatagca tacatgaata
    29581 aaacttcccc attcgctttc ttacgctttc tgaaccagat aagtataaga aaaactccca
    29641 aatcccataa tgattcataa agaaatgttg ggtgaacagg tttttcagga ttaatattaa
    29701 ttcccatctg ttttagcttt tccatattaa gagcaagttc gtatttgata gcatcacccg
    29761 tcattcccca tggaagatcg gtatttactc catatgcctc ctgattgacg aaattgcccc
    29821 accttcctat agcttgtgcc atgggcagat atggtatacc aaaatcaagc aattccagga
    29881 aattgatttt ttttactctt gtatatataa aaactgccag cagtgctcca agtatgcctc
    29941 cataaatggc tagtccgcct ttcctggtgt ttattatatc cagcagattt cctctaaaat
    30001 cgtcccagct gaatgcaaca taataaagtc ttgcaccaat tattgaagcc ggtgtaaggt
    30061 aaagaaccgc atcaataaaa ttatccgggt cgatattata cttcctgcaa tgcttaagtg
    30121 ccagcagtac agcaagaaga aaagcaaaag caataataat cccataccaa tatataggta
    30181 tattaaatat ccaaaaagca atcctgttta ttaaaaacct aagccctaat ttagggaact
    30241 ctattgtacc acccattaca tttcccccta tctcattaaa atggtgttat gacagacatt
    30301 gcaaatctat ccctggtaaa atgttccttc ataacactgt taacatattc aaagtttatt
    30361 ttatcataaa tatcaaaata atcaaatata taaattcctt tgaaataagc ggaaataaaa
    30421 ttccctgaaa tattttccac agagttgaat cctctaacgg tcctgccgta cattgccttt
    30481 ttaactcttt cataatcatt ttggctaatg ccattataaa taaaactgtc aatgcactct
    30541 aaaattcttt cttttacttt caaaggattc tgagactcac catttattac tgaataggca
    30601 taattttctt cggcaacata atcaaattcg aaatcattat ttataagtcc atcttcataa
    30661 agactgataa ataaatttga acttctgcct ataattattt ccagaagaat tttcatggca
    30721 ttttctcttt tcaaaagctc tttaccacca gcactgaaat taaaattatc tttaaaaccc
    30781 atctggaata atgggattga aacctgcatc ttttcctcaa tataaggctc atttatattt
    30841 tgaggttctt caggaaatat tctttttgct atcaatttat tttcactgtt taatatgctg
    30901 ttttcaattt gtgagaatac actatccgtc tcaacatcac caacaacaga aacaaccata
    30961 tttgacatgt tgtaaaagtt attgtaacac atatacaagt tgtcttttgt tattctgtca
    31021 atgctttctt gtgtacctaa aatttcattt ctcacataat tattcttgta taataccttt
    31081 aagaaattaa aaaagcttct ccatcggggg ttatctgcat acatatcaat ttcctgtttt
    31141 ataatacttt tttctttttc tacgctttgt tcagtaaaaa atgggttctg gacaaagctc
    31201 agtaacaagt taaaattttc agtgaaccta tcggtacatg aaaacaaata gacagtttgg
    31261 ttaaaacttg tgtaggcatt aggttcggaa cctaatgcgg aaaacttgtc cattatgctt
    31321 ccttcttttt ggtcaaatag tttatgttcg aggaaatggg caataccttg aggcactttg
    31381 ataacatcat cactattatg ttgaataaaa tcagtattta ttgaaccata atatgctgca
    31441 aaaactgcat acttttttga gtatcctttt ttaggtataa caaaagcatt taaaccgctt
    31501 ttatgtgaat acttgtgcat agtctcacct atatttttaa actcaattgt ttcataattc
    31561 atttaaccat acctcctgca tgtaataagt agataagttc cggctgaatt ttacctgcaa
    31621 ctcttttaat atcatcagtt gtaacctttc taatttttgc cagtaaagag tcgaaatcat
    31681 cacttgtgtt tcccagaacc tgacttaaat aataatctac tatctgcaat tgattatctt
    31741 ttaaagaatt aattcctgtt tcaattgatg ttaaagcaga ttcatactcg cttggggaaa
    31801 tatttcccga cttgatgcta tctacctgct gaaggattaa ttctacagct ttatcattat
    31861 tctgagcatc aataccgcag gaaaccacaa acattccttt aaacttatca aattgtgaaa
    31921 aaatataata tgccagacct gcaccttccc ttacatattt aaatagtttt gaattaatac
    31981 ctccacccaa gatattgtca cagaaaagca gaggataata atccggatct gaagagcaag
    32041 tgttggttaa gaaaccaata gataattttg cctgggttat atttagaact tcattaacct
    32101 ccctgatttt tccggtattg ctgatgctgt tactataatc aggaacgtta ctgtcagtac
    32161 tgatattgac aaggcaatta ttcttattac tcccggttct tatactaatt attttgtttt
    32221 ttacagcttc catcttttgt gttgaaatat caccagttac atatactttc agagggtaac
    32281 tgtccataac ccctttataa tgctcataaa gattaatttc atttatttca tcgagacctg
    32341 taacatcacc atattcgtag attccgaaag gttcatccct gcacatttcc tcaaagcatt
    32401 tttctatggc atattttact ttatcattaa ctctgctttc aattaacctt ttcaaattat
    32461 ccttttcctg attgacagat tctgctataa atgaaccatt atcaattaca ggatcagcaa
    32521 ctatatcaag gagcaattca aaagattcct caaacaaatc ttttgaattt ttttcgcttg
    32581 tttttttagt tatatatttg tcagatatac attcactgta aaagaaaata atttgatatt
    32641 cgcctttttt tattactccg cagtcaaagg aagccccata cagctcctcc agatgccgag
    32701 atattttgtc catttccggg aactttttgc atccccttct taaaatagct ggtatcattg
    32761 cattttttga tactgtttct ttttttaagc tatctatgaa aaagaaagtt acagtattgg
    32821 ttttgaattt ttcggtggga atataatata tttctgtgtt taaaaacgaa tggaacatat
    32881 ttaccgtcct ttcctaataa ttttttactt atcagtatgc agcacacttt tcagcataag
    32941 cctaagtatt tttgctgcta gaagtctgct tggaaagcct gagctatcaa agcggactac
    33001 cagacggatt tattatttcc attttttcat acaatatgtt tttcaaacag atttctagct
    33061 gttgatacat cagcttttat ctgttttacc agttcctcat ggctcttaaa tttcttttca
    33121 tctcttattt tcctgataaa aaatacctca ataggctttt ggtaaatatc tccttcaaaa
    33181 tcaagtatat gagtttcaat gctgactttt gaagtagagg ttttaactgt aggttttact
    33241 ccaaggtttg ttatactatt ataaatttta ccgtctaaaa gtgtttttga tatatatact
    33301 ccaaaagatg gcaaaagaag gtattcttca ggagtgatat tggcagtagg gaaaccaagc
    33361 tttgttccaa tacggtagcc tgtttcaacc ctccccatta ttgagtaatg tctcccaagc
    33421 ataaaaagtg ctttttccat atcccctctg gaaagatatt ctcgaattaa tgtactgctt
    33481 acgacttcgt tatttacttt tatgggtgga actattataa ctttaaaatt atacttctct
    33541 cctaattgcc ttaatagacg agcgtctccc tgacctttat atccaaaagt ataatcaaag
    33601 ccggctacag ccagcttaat cccaagttta tctaccaaaa cacatttgac aaattccaca
    33661 ggttccattt tagaaaaagt ttcatcaaat tcctggtaaa ataaataatc tacagaagta
    33721 tattcaagta tttgcatttt tttttccgga gtcattaaca ggggagtaaa atggtctttc
    33781 tttaaaatat tatcaggatg ttttgaaaac gtatatatca ttgactgcaa gctgcttatt
    33841 ttgcattcat tttttaatat atttgtcaac gccatatgcc ctatatgaag tccgtcaaaa
    33901 ttaccaagac ctacacccat atcttcacca atagacatat cattaatagt acttaatact
    33961 ttcataattt tctcctctat tgaaacatct ttttaggctt aataagatgc tttccatctt
    34021 tttttactat ttctccaagt gctacaaact tcatattgct gtcatatacc cttaataagc
    34081 ttccagcaac atagtccaca tgattaaaat caaaatcttc tatatttata aacgaaccat
    34141 ttttaaaatt attttcattt ttcttatcaa gacatatgca tgtgcattct ttaaatattt
    34201 catctacagg tataagcata tggtgtattc tgtcattttc cactgcctca agaatatctt
    34261 ccagagtatt tgaacaatct atatcaaata ttcctgtcct ttttcttatc aaaaaagaca
    34321 tatgtccgcc acaacccagg caatctccaa tatccgaaca taatgtcctt atatatgtac
    34381 cctttgaaca acaaacatca aacagtattt tattttggtt aatacttact atattaatag
    34441 aacgaataac tacttttctt ggttcccttt ctatgacgcc gccttccctg gcaatatcat
    34501 acagtcgtct gccatcaact cttactgcag aatacatagg aggtacttgt tcaatttctc
    34561 ctataaaact tttaatgact tcggctattt catctacaga tacatttacc tgttggcttc
    34621 tctctacttt ccccaatgag tcctgcgtat ctgtggaaat accaagagta agttcagccc
    34681 tatatatttt atccatattc ataacaaact caattgcttt tgttgcatta cctgcgcaaa
    34741 tgattagaac acctgcagca cccgggtcaa gggtgccggc atgtccaatt tttttcactt
    34801 ttaatattcc tcttaaatat gcaataacat caaatgatgt catacctgga ggtttcaata
    34861 cattcaacaa tccatttatt ttttgcactt ccaacctatc acctttgtta ttatgtattt
    34921 atgttcatct gatattttgt atttatgatc atcacttttt agatcctttg ttggctttct
    34981 cttcaagagc ctctataagt tcttttaata ttaaattcct gacatcattt aaat
//
LOCUS       DAOSDD010000013        34927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_623_length_34927_cov_5.228722, whole genome shotgun sequence.
ACCESSION   DAOSDD010000013 DAOSDD010000000
VERSION     DAOSDD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 34927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34927
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_34927_cov_5.228722"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1105
                     /locus_tag="PK733_02210"
     CDS             284..1105
                     /locus_tag="PK733_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ09390.1"
                     /translation="MFRICCFADEISSDLNVQINVMKKNKIKYVELRSVWNKNVLDLS
                     DNELDMVKEQFSDNGIKISSIGSPIGKVDINDDFEKHLDRFKRAVDIALKMETCYIRI
                     FSFYMKKEELDIYQQTVINRLKQMIEIARENSLVLLHENEANIYGETSARCLNLFNSL
                     PYTNFRAVFDSSNFVVAGEDVFNESFSKLKDYVEYIHVKDSKKGSGEMVVAGQGDGRI
                     REIFDELRDKEGMFISLEPHLTHAGKYRGFTGPELSEKALEALQGILMELKIEFE"
     gene            1237..2409
                     /locus_tag="PK733_02215"
     CDS             1237..2409
                     /locus_tag="PK733_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09391.1"
                     /translation="MDRKTMVSVERVRAAINHQEPDRIPLDLGSTKMTGISIKAYKNF
                     IKYKGWQELDENPEILDQVQQLALVREGVLEKLGVDTRGLIPSAPSNCKTEYREVGDY
                     IEFTDEWGVGWKKPITGGYYFDMTSHSMSGEMNLSQLDDFTWPDPLDETRTAQFPTRI
                     EEIKKHGDYAFLMHGVSAGILEMALRLRGFEQFFMDLILDPGLVCGILDRLVEIKMAY
                     WGKALEKVEKDVLVAIEADDLGTQNSLLISPDMYRKFVKPRHKELFSFIKDKVPGIKV
                     FLHSCGAIKPLIPDLIEAGVDILNPVQVSASNMNTKDLKKEFGDVLTFWGGGIDTQKV
                     LPFGSPDQVKEEVKRRIDDLAPGGGFVFSTIHNIQADVPPQNIEAMFEVLEQCGIY"
     gene            2448..3497
                     /locus_tag="PK733_02220"
     CDS             2448..3497
                     /locus_tag="PK733_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09392.1"
                     /translation="MSIKTAILGYGRSGSTLHADPIERLPEFDLTAVCDIDPEARSKA
                     YNRFKCKIYEDYKQMLKDEELDLVVIVTRSDQHCQMTCDCLEAGKNVLVTKPWALNIN
                     EAQRMAATAEKSGKLLMPWLPARWGCDLLRLRELIESGVIGKVFQIRRSEFSFGIRHD
                     WQTERKYGGGYLLNWGPHLVDQPLQLAGKPVKSVYGELKQIINPGDVEDVFFAVIKTE
                     DDITVVSEYNIGSDRLPNWVIQGDRGTIYVKETEIEIHKVNYPESFDPKSYRNPVTVE
                     VIRDDAKGTNMVTMGNRYGDSMVIYPHIAKAMRGEEPYMASLESALTLTKLLDAIRKS
                     SETGQVVSVFSTAVR"
     gene            3827..5764
                     /locus_tag="PK733_02225"
     CDS             3827..5764
                     /locus_tag="PK733_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ09393.1"
                     /translation="MNIKDIASLANVSVSTVSKIINKKDDAISDETRKKVLEIIKKYQ
                     YEPYSEIKKKQNPHSGLIGILIPPNANEQLFELVSCIEQDASRNGYSIVLKISDFQDS
                     MSKRSIQVIIGKKADGIIVYTDNKKDETIYSLFQDQDIPVVLLNKTNIHMQQNIVSVF
                     CEKKQIGLMAANYLISKGHHNIGCILSSDLQLSDNEIYEGYIQALYNVDILSDEAYVI
                     IDSDPQKTIDSVKSLLNMNVTAFLCEDSKQALALYGMLQQFGLSVPRDVSIISAGDSA
                     ISEIMTPKLTAIRYPILEVSNTAINHLINLIENKKKPKELITDVEPILIERDSVAAPN
                     EKKGQRKKIIVIGSMNMDINIYTDDFPEYGKVLVSSRIALIPGGKGANQAVGVGKLGG
                     QAYAIGRLGNDNDGKEIYTSLANNYVKTDGIIFDTLMPTGKAYVSVAKDGESGIVLYQ
                     GANSRFYADQIEKFKHLFEDAEYCLISTEIPYKTVKYAIKTCNKRNVKVILKPSMIQN
                     IDIELLRHIDYFIPNENELNQLIPGENTVYEKADYCFKQGVKNIIVTLGAKGCYLKNK
                     DYEMHFPAADFTPVDTTGAADAFISALAVYLSEGNSITQSIRFATYAAGISITREGVQ
                     PALPDRTALDIYFDKIEPSEK"
     gene            5833..6753
                     /gene="rbsK"
                     /locus_tag="PK733_02230"
     CDS             5833..6753
                     /gene="rbsK"
                     /locus_tag="PK733_02230"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOJ09394.1"
                     /translation="MKKILVVGSLNIDFVINVDEMPKTGETVLGNNLEFIPGGKGANQ
                     AYAAGKLGASIAMIGAVGRDIYGTILVDNLTSVGVDTTAIARLDNQKTGSAFIVVNKQ
                     GHNRIIVISGANKCISIDIINRHINMIQESDIIIMQLEIPMDVVIYVAKIAKQMGKLV
                     ILDPAPAPEFLPDELIQNVDILKPNETELARLTGCSIYTIEDIVSGANKLVNRGVPTV
                     IVTLGERGAVMVNRDGWVHKTVSSVKAVDTTAAGDGFIAALALGLTREMSIDEAIDFG
                     NSVSTLVVTRKGAQTSIPTMDEVKLLLKHI"
     gene            6852..8432
                     /locus_tag="PK733_02235"
     CDS             6852..8432
                     /locus_tag="PK733_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09395.1"
                     /translation="MKKVLSLILVALLITGILSGCVVKSNETKIPDAASNETKASAEA
                     KKLLPKPTTFTIMTFEHPSTTVNPYGIRWQEVFDKTNVKLEFDITPQGDFNAKLATVL
                     ASGVISDITWVKQNNTTEYGKKLFMDLTDKLETELPNYWRKVKDDINFLKTKVEGRVY
                     STYFLQNDNYPGGLVPVIRYDLLEKHNLPMPETFEQWFEVMKQLKNLYPDSTPYSGRG
                     GRNTPLNLEYAMGAQRNLNYDLVKQRYVYGVLEPEYRKILEFLNKCYAEGILDNGYMN
                     TNANTWAEGAANNKIFFWYDNGGFATSQTLALRQNNPNALMQIMPLMENAQGEKRGQI
                     YAKHWYNEQLVFSKNTSKPEELLKFINWMYSDEALLINNLGKEGITYNVLPNGEVELP
                     KELVDKYKVSTSPDDAWQSDLGVGYLNVTPFVGYTPLLGKALGTFNEGFYDVLLPDLK
                     AGYYKEQWVAVVLDPEVKKSINELEKQINSLINNQIPKFIDGTRPLSEFDQFVAEVKK
                     LGAEDVLKAYNDAVAALK"
     gene            8576..9682
                     /locus_tag="PK733_02240"
     CDS             8576..9682
                     /locus_tag="PK733_02240"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOJ09396.1"
                     /translation="MINNYTDKPLVKLPSECKLIFNAMKGWSYSHHPFITFFKGKFHA
                     IWSNGRVNEDDCGQRVMHATSENFIDWSVPEPLVNSIMGIGSELVLTACGFYQHNGVL
                     NAYFGHYEYDPSSLQAKNLRPKGDIAHINVGLSVVSTTDGVNWSAPRELGIPMGANFG
                     PQPTKSGRLIISGSIVYPYSDDISGTGKFTLTGIYESIDNRVRDDSEAIEVTIKRKQW
                     RDARLICEGSFFQTDDGVIHMMLRSNTPRLWCTESTDDGTKWSEPFPTEFSDDHAKFH
                     FGRLPDGRFYYVGNPIPRSGRNPLVLCLSEDGINFNMHYILRDEPYKQQFEGMYKGGL
                     YGYPCTLIHDGYMYVIYSKRKESVEATRFQLSKI"
     gene            9705..12605
                     /locus_tag="PK733_02245"
     CDS             9705..12605
                     /locus_tag="PK733_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09397.1"
                     /translation="MWVKRRVLLIPVIICVSILITSIAIIDAASLQQNINSLKSLNAD
                     NLSNVSIQNEFFSKVFSEYPETLAGENIEIDLLNPTDGTAKIENNIGGNPKAALIWEH
                     LTNEYKWKFKVNEAGQYVITINYYILGGSGNNAIRSLKIDGKTPFSEADNIVFYHDWE
                     DKGEIIKNSIGDEVRPSVKAKKSWQHVIIKDGSGFYSIPLVFYFEEGEHELSMNYVSQ
                     DMAISDLVFVPYKEIKSYEKVSKSHNSETKAKEIMLTFEAEDTVITKNDATLRIESNG
                     DPATTPKAFGYRVFNTIGGWRWRRGNQSITFEFEVPEDGLYKIGFRFLQTWNDGLPSY
                     RTIAIDDEVPFKELLAYRFSYNSSWETEVLGGKNPYLFELKKGRHSITLTVTMGELTP
                     IIQSLYGDILLISDMLQEIVKLTGNAPDPNYDYQFFKYIPTLENDLYTLIDSLDYKYQ
                     MINNITTKSSTMSGNFKSIKEQLISMVKNPFSIARKLNQITQAQSSLGSWYLELQNQP
                     LLLDKFSVATPDIEIKYRKSNIFERMLTEFQNLRLSFVKDYNNMASIVKDDVEVKSVI
                     DVWIARGTEWAEIVKEMADENFTPETGILINMNVVPSSQLNTGSANAIMLSIISGSAP
                     DVAMGVDSGSPVEFAIRDAVVDVSQFSDYEEIEKRFLKNIMIPYKYKEGVYAIPETMD
                     FTCLYYRKDILEKYNILIPDTRYELYHYLLPELYQNGMSYYQPHDFTSFLLQNGGSYY
                     TEDGLYSALDTPKAFQAFREYTEMFTHYSVPVNANFFNRMRTGEMPIGIGGYGLYMQL
                     STAAPELVGRWGIAPIPGVLEGDGSVNRSNAGIAAQCDIILQQSGKKKEAWEFIKWWT
                     SKEVQIQYARELEALVGIEARWNTANAEAFESLEWRQEDLDVFNEQWKWANETPVVLG
                     GYFTGRYINNAFNSVVVAGNKTPRDALEDAVREINKELKMKQEEYGVFRDEN"
     gene            12595..13548
                     /locus_tag="PK733_02250"
     CDS             12595..13548
                     /locus_tag="PK733_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ09398.1"
                     /translation="MKTEEVIINTHIDKKISQTKFGEIKMFIHKHWVGYIMLVPFFIL
                     FIIFVVLPVIIALLLSFTDYNMLQPPKFVGLKNYELLFLDDKIFLTALGNTFLFAFIV
                     GPIGYLMSFIAAWVINQLKLRNAFALAFYAPSITSGVAMSVVWLYFFSPDRYGLLNNL
                     FINAGLIDNPILWTQDPNYIMPVIIVISIWMSLGNGFLVFLAGLQNINKEHYEAAAID
                     GVENRYQQLKYITLPMMKPQLLFGAISSITSSFGVFDIAVSVAGLPSPNYAGHTIVAH
                     LYDYAFIRFQMGYSSAIATVLFIITYSFGHIIMRLLSTKDE"
     gene            13562..14449
                     /locus_tag="PK733_02255"
     CDS             13562..14449
                     /locus_tag="PK733_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09399.1"
                     /translation="MVKFKMTKSKMNVLKVKKEGKWTFVYIFMIALVAFTSLPLVYLI
                     STAFKPLNELFLFPPRFFVKEPTLKNFTDLMLSLSSSTVPFTRYILNSLYVTVITVIL
                     TVFVSTLGAYSLVKHNPPGTRKLFKFIIATMMFSPYVTQIPRYLVVNSIGLVNKIAAL
                     IIPNIAVSYNFFLIKQFVEQFPNELLEAARIDGAGEFYTYWKIVMPVLTPAWSTLIVF
                     SYVSSWNDYFSPLIYINNQALKTLPLALRTISGGGASIGRAGAVAAATMLMTIPTVVV
                     FTSMQNKVMETMVHSGIKG"
     gene            14464..16599
                     /locus_tag="PK733_02260"
     CDS             14464..16599
                     /locus_tag="PK733_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09400.1"
                     /translation="MKIRVLVLTIIFIMMFSSAAMAYEVDYIINDGEHVPIPQPYIYS
                     KTINSIKYNDEAAIKYFNQPADIFIDKNDMIYVVDTKNNRIVKLTYDGEAKMIFTEAN
                     GKKFNQPQGIYVDDNFDVYIADTGNSRIIQLSADGSFIKESLKPESGMLSDIAIYSPT
                     KIAKSITGGLYVLLSENIMVIDENNRFRGFIGQTKIGFDFTEWLLRIVASDVQKKSIA
                     KRLAASYDNFCVDDKGLIYAVSRDTVEGQVKILNSIGNNIYRKYTSVDDNIDTVADYI
                     KNFFSGNIISKSFQYGESVYTTKWERPVFIDIAVSSDGIITAIQKQNGKIYQYDQTGN
                     LLAVFGGLGSTQGKFAIPSSIAIDSQGRLYALDLSYGNIQIFEPTEFIKLVQQATIMY
                     NNGNYDEASAIWEEIMRYDETYPLAHYGLASTNYKLGNWKAAMDGYFNSNNRVEYSKA
                     FTEYRYKLLKNNFFIVVLLSLAIISLLYFIIVRMWRNTLVVLDSFESYKLQKLNFLNG
                     LVMGLGILYRPGRALDNIKSGRGRLNVKCASFIWAVVVAVRFFFIYNVHYPLQDIEIQ
                     DVNFLLEIVKITFPVLSCIIAAFLISSQFNGESTLEENFVAMTYCMIPYIIVTIFATL
                     FSQILCTQEKMLFALFVNGVTIWQWWLIIRAVKRLNDYNIRHTIFVCVITFFAVILIW
                     FTGIFAYTLVARMIQFIEEILLEAQLVFF"
     gene            16611..18836
                     /locus_tag="PK733_02265"
     CDS             16611..18836
                     /locus_tag="PK733_02265"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ09401.1"
                     /translation="MKTLKHIILFVLIIAVCFSSVNSSGADNSITNSTTISNSSVFES
                     DLPDQYLFICENDRFSLSYREDITSIALTDKLSGRMWYSAVNEELYDIKNSSKTWQTY
                     MQSILSIKYVSKKDTRGNFINDYSAAKDNAVFAEKLENGLKLRISFRDSAIDLTLKIE
                     LEEDHVKLSVPIDEIKENGEYSIVSVELLPFFGAAAKDADGYIVYPDGSGAISYFNET
                     DNKHQFTKQYTIDVYSLLDHEKIFDETNEENCAMLPIYGIKNEKNAFLAVITKGDSSA
                     KINVNTSVNSSAVKLNRASFEFVYRNQYRIYLSNIIINGRSRTTNPYGVKVDKDMIQL
                     DCEVKLFFLNDDVANYSGMANVYRDYLIKEGLLKKSKLQDEVGLALNVFMGTREEKTF
                     LKKFVNMTTFDEVNRIASDYLNNDIANLQIMLRGWSKNGYYNQPNIATPDNHLGGSAG
                     LKRLAALDKENDKLFLYLETNVNSASKSSGGFSTGRDVVLQGNKIPVSHNKSKFLINA
                     KKMESLYENALIKMSANLPIGVALAKAGSFITHDYNKSFGLQRSDNIEVWKNMISQTY
                     SNVAAQGGNLYTLALVDRLYDIPIESSMNQITDESIPWYQMIVYGSIPYTSEPGNLSY
                     DLELLKLKWIEYGCMPYFEVTVRNPLNLKNTSYNTLFTSENSKWREVILDIYNEWKSK
                     LEPIQNTFMIKHEKLTEDITQITYENGYQVIINFSFSKINYNNMEIPARDYILVGGAY
                     N"
     gene            18833..19723
                     /locus_tag="PK733_02270"
     CDS             18833..19723
                     /locus_tag="PK733_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ09402.1"
                     /translation="MKRIRIKSETKQALIGLFFITPFMIGTIVCFLYPVIASFLLSFG
                     DTNKEMAGFHINITGFQNYYRAFMEDTSFVPMLLNVLKQTLTQTPLIVIFSLLIAIML
                     NKVKKLKGFFRVVMLLPFLLGTGEVLNHLTGQGVDKQIISISNSNIIPREFILYLGSD
                     IVKLLDTLFGTIVLVLWSSGVQILLFLSGIQSITPSLYTSARIDGANEYDMFWKITLP
                     MISPILLLNTIYTIVNSFTSKLNPILDYVKQYAFVNSQHGYAAAMGWIYFAFILLIIL
                     IVILVLGNYARSSGVKGRKE"
     gene            19724..20707
                     /locus_tag="PK733_02275"
     CDS             19724..20707
                     /locus_tag="PK733_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09403.1"
                     /translation="MNTGETIINMNYFVKKHDLKHDLTSHRWILKIKKYLPRVILYTF
                     LLAISFIFIYPFLYMIVTSLKSNEDLNNFIVVWIPRTLHFNNYLLAAKLMNFSVTFKN
                     SAITTALATIGQLISCSMAGYGMARFKFPGKRLAFFIVILALIVPAQAIIVPQYLLYS
                     KLGWLNTYFPLTVPAFLGFGFKGALYIFIFRQFYLGLPKELEEAAGVDGCGFLRTYIK
                     IVFPIARSAYMVVLVLALVWHWSNYFEPAIFVSRTNLRMLSSGINNIADTLRLPPETL
                     VSMYNISDENTLNTAVLMAGTFIVVLPILIMFGILQKQFIQGIERTGLTGE"
     gene            20850..21740
                     /locus_tag="PK733_02280"
     CDS             20850..21740
                     /locus_tag="PK733_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ09404.1"
                     /translation="MDTKKKKVLFDTDIGSDIDDAVALAYLLRQPMCELVGITTVTGD
                     TVKRAQLASVLCLAAGKGDVPIYPGCRMPLLDNELQPHVPQADVLPLYNHQKEFPMHR
                     HIQFMIDTIRANPGEISLLAVGPMTNIGLLFATDPEIPGLLKELVLMCGVFTNTLPRV
                     GPREWNAYGDSHSTAIIYNTRPAVHRSIGLDVTCQVTMGSNEVRERFNVDLLKIVLTM
                     AEIWFNRRSIITFHDPLAASTLFDESICGFERGKVDVELLSDRVRGMTYFKADKENGP
                     HEVALRVNKEKFFEHYFSLF"
     gene            21793..23361
                     /locus_tag="PK733_02285"
     CDS             21793..23361
                     /locus_tag="PK733_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOJ09405.1"
                     /translation="MQLTINKKYLLLPVSSGAVKKVLLFYHNKKLVYELSTRIDYLAP
                     EYFFPVDMHPFQGLTLDILCNPDMKLDLHMTDIIPELPESTKRYRPFLHFTVNQGWHN
                     DPNGLIQINGEYHMFYQYNPADSEWGNMHWGHAVSKDLFHWQEQDVALYPDEMGTMFS
                     GSAIIDSKNSSGLSKNGEPAILLFYTAAGNTSEHSKYKLFTQCIAYSTDRGKSFVKYE
                     HNPVVQHIQANNRDPKVIFSTELNKYVMALFLESHQYCLLVSDDLLKWEKLQDITLND
                     DSECPDLFPLAVNGNKENIKWVFCGASDFYLVGSFMKGKFVAEQEPKRLFHGRVSYAA
                     QTFSDVLDGRRIRIAWDRLKIPNSPFNSQMGVPCQLNLIEEDNQYYLCAQPVSEIASL
                     YKNVHIEENLLVETNEPFKLDLQNKAYDISLTISERLENNFSLTIFGQIISYNFDSNQ
                     IIFKDNKIPAKIGKGPFTMRMIVDKLSIEIFNDLGRVAICNSCICDYNLNNMEVHSDS
                     KLLIDKLLISEIEI"
     gene            23549..24907
                     /locus_tag="PK733_02290"
     CDS             23549..24907
                     /locus_tag="PK733_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09406.1"
                     /translation="MKGYNAWNYAPYIPAGERTGTSVPYICRLAPSYNKVEVEWFHNN
                     YEGVHRIFYSKVNSGSWQEQLISERTFTIEGLEQDMEYMLYIKADNGPQSIVRVIRTG
                     FVPGAVVNYLHPKDNQYSFSGQYLCSPSLVKLPSGVLLASMDVYGRAAPQNLTLIFRS
                     KDGGSSWQYVTDIFPCFWGKMFLHKDALYMLGVSNEYGDLLIGRSDDEGNTWTAPTVI
                     MRGSSNIKENGNHKAPVAVLQSHGRLWTGSEFGCWHKKQFKSSLYSIDENDDLMQANN
                     WTCTGFLAHNDTWPGAEQECAGGIEGNVVESPKGEIFNILRYSKSKALMLKANPDNPE
                     EGLTFYKFIEFPLAHTKFEIQRHDNGIYYAVGNKFPGRTVLSVYTSEDLEHWKFKCDV
                     MNYYQMDIKEVGFQYPSFYFDEDDLLVLSRTAFNNAANFHDSNYITFHRVKLCVHQQY
                     TR"
     gene            24993..25507
                     /gene="rhuM"
                     /locus_tag="PK733_02295"
                     /pseudo
     CDS             24993..25507
                     /gene="rhuM"
                     /locus_tag="PK733_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(25560..25721)
                     /locus_tag="PK733_02300"
     CDS             complement(25560..25721)
                     /locus_tag="PK733_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09407.1"
                     /translation="MEHNEMGYIKKFIRENYNQNISLQTLAELSGYSYHHFRHKFKEN
                     LGLSPMSYI"
     gene            26583..27482
                     /locus_tag="PK733_02305"
     CDS             26583..27482
                     /locus_tag="PK733_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09408.1"
                     /translation="MYINPYSSAAEGKGAWMKSNFHTHAGTGPDTCGAYDLEEVVKHY
                     KAAGYMILCISNHDLFTDTSHLKGKYGIGLINGFEYSKNLHMLCIGCKSLIEGEHQAA
                     VNECVNQEGFVILCHPNWQHKEYWPWGKIDALKGYAGIEVFNGVIGRLTGTGLAEDTW
                     DYLLSGGKLVWGFGNDDFHRWHDLNKSWTMIYSESNEENDVKSAIRSGSFYLSTGLFL
                     NEITFDNNIIKLRASENACYPKNYQYTFIGKSGEMLHRQEGEFGEYKLSGNELYVRVK
                     VMGEQGYVLWTQPIYNKNMFERP"
     gene            27653..28504
                     /locus_tag="PK733_02310"
     CDS             27653..28504
                     /locus_tag="PK733_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOJ09409.1"
                     /translation="MDNNRNVLLIIKSLYPSLHRVEKKIAGFILENPETIIYMPIAQM
                     AKDLEVAESSIVRFCKIIGFDGFKQLKISLVKSLENPEKIIYEDINKNDSQHEIINKV
                     FSSSIRTLQESLQTIDENQFTMAVEAMLKAKRIEFYGVGTSSTLAQDAYYRFMRIGLP
                     VYAVVDPHVCRVSASTMDNTCLAIAISYKGRTRDTVETLEIAKSKGARTMCITCFPQS
                     PITKVSDIQIIMPTSEAKIMREAISSRIAQIAIIDSLYTAVALEKFDTVVDHIEDLSD
                     ILSSVRL"
     gene            28620..29876
                     /locus_tag="PK733_02315"
     CDS             28620..29876
                     /locus_tag="PK733_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ09410.1"
                     /translation="MENRTMKYWQKRIFILCWAAYTIAYLGRINLSIAIPLLEDSLTL
                     DKTNLGIIGSAFFWVYALGQLINGRLGDKFASRDFVFFGLLISAILNLLFGFFTNLIV
                     MTALWAFNGYFQSMLWGPLMKTINQWFPQKQKSKMALYMFSSVVSGFILTWGGLGQIS
                     SYMGWKAVFWIPGLILLLYSFIWYLYARNSPEEAGLCLKDTKTYTEDTELNADKLVFH
                     TRLYLIALAGIPLGFVREGISLWAPAMLLDTFQLNLKSTMSAALFIPLFNFMGVIAAR
                     LLIIKLQNNETKLVAIFFAGGIITCTLLYFSRNSSIVAYLVLLAVCSLSLYGASSIIT
                     SVIPLKYRMTSSVAGFLDFSIYLGAGLSGIITGALSNKFGWSIVVLVWVIAGAIGSIC
                     MYMAGRRCSNRSTVAKVANADLSLIE"
     gene            30039..31955
                     /locus_tag="PK733_02320"
     CDS             30039..31955
                     /locus_tag="PK733_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ09411.1"
                     /translation="MAKLINDAINAINIKKTSSRIYSKIKKVISNCVNNVSSIAKEST
                     GIFKKTDTRQQFIILFAFSTIVLVATVCIISSAYSNTILGSNEKYINNVVTQYKNEFT
                     HNLSEIDQLVNGLAYNPAVKNYLTIKDNPYQRHVYASELITYFYYLKSVKVAIKDMTV
                     FRLDAGKVTYYDSIRPESLIVDLFDQKPHSYCYGVVEYGGEHGGRHYRNLLSGCSVYN
                     KNYLTDEVEKIGTIVVSIDPYGLSIKLEELEGVESVKYMVYDEKNNIVIGNIDTNNAE
                     IADIINKAENNHNANHFETKRYKLNIVEVEGLRGRILTIVDKNLLLGPVKQAVFFSGL
                     ILFIAIILLVLMYYLAAGNIVMPINDIIVFLQTMRKEDISKLDKRIPIVGNKDIRLFS
                     LEFNNALDEINALTYKLIDKNSRLYEMEITKKQSEIALLRSQINPHFLYNTLEAIRSI
                     AVIRDVPEIKDISRSLARIMKYSIKGYENVTLAEELGIIKDYIQIQNTRFDNKFSTEF
                     IIEPEALACTIPKMTLQPIVENAISHGLELTPDAGHLLLHAYIENENRLVVRIVDDGV
                     GIDEADLASITEKLESEDYSFYLNHRAGIGLVNVNSRIKLTQGKNYGVKVKSQKDKGT
                     EVTISFLAEVSNNV"
     gene            31948..33369
                     /locus_tag="PK733_02325"
     CDS             31948..33369
                     /locus_tag="PK733_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09412.1"
                     /translation="MYKVILIDDEQWIIKSLKKSIPWNQYGFEVCATAADGKIALEKI
                     NEFHPDVVFTDIKMPYMNGLDLIAKAKQSHPDLLSVIISGYAEFEFAKTAIQHKVDGY
                     CIKPIEEEEIIDHLKNFRKELDNLKRSRNNLDLLNCIERNDKKMFKEIYPCAGSICII
                     VSIGDKITYLEGKPKALYCNVSMNKWLYFLPEPINMQQIEGNLSKNIKGVGIFQINLQ
                     TDDLVKGIDEANTRAQQYFFNEDNKVFAGDYDKNKIKTYNQLLNGIIAQVKNKDITAL
                     SRSLEEIKIILNSINIVELKKSYSILNTLKYLNITGDDLEEDETWMDQDIIFLNNWSN
                     VEEYFEYFRHEIKILVNIKHESIQIYSGNKLISDVINYIKDNYMKDITLYNLSTEFNI
                     NQSYLSQLFKNETGVNFVNYLMNYRISKACSLLLQTDMSINNIAQEVGYDDYLYFRKV
                     FKKVNNMTPTEYREQKMRLINNE"
     gene            33554..34636
                     /locus_tag="PK733_02330"
     CDS             33554..34636
                     /locus_tag="PK733_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ09413.1"
                     /translation="MKSKFKLILLLMLVITFFTGCIKDITVSPAEQTTSSVSGTTPPA
                     KESENEIPIEYRTEEIYDRTNDNGKLVFYFMQIDEIDNTTKWGDSTLICFPNGETMIV
                     DAGKSFAGEHILNSLERLGIDRIDYAIASHMHADHYKGFYTIMKNIDIGVMYTPDFPC
                     FPDEQYTNNWENALKSYGVEHRHLEMGDTLDIGDVHIEVFGPELTEANMNTVLSLGKD
                     SPRIVNNLTSIVMKLTYGKTSALFTGDIYMIREIELTELFSDKLEADLLKVPHHGHDT
                     SGSKILIDTVKPKVAVAMGNILMDPYTTYAQYVRADVKIYITVIDGMVKVTMDGSDME
                     VITEHDRQPNFRKTYDDKRIKGLTNY"
BASE COUNT        12139 a         5095 c         6848 g        10845 t
ORIGIN      
        1 gctaaaatga atttaagccc ataaaaactt tttcatcaaa aaagttttga tcctgcaagg
       61 gcgaattcat ttcgcacgta gcaggaatgg agcttagttt gaaagaaatt gataaatttc
      121 tgaaaaacta atgcgcatgt gcatattttt tgcacaagaa ttatgaaaaa ataaattgca
      181 cgcgcggcta aaatgaattt aagcccataa aaactttttc atcaaaaaag ttttgatcct
      241 gcaagggcga attcatttcg cacgtagcag gaatggagct taaatgttta gaatatgctg
      301 tttcgctgat gagatatcat cagatctaaa tgtacaaatt aatgttatga aaaagaataa
      361 aattaagtat gttgaattaa gaagtgtctg gaataagaat gtcctagact taagtgataa
      421 tgagttggat atggtgaaag aacagttttc tgataatggc atcaagatat catccatagg
      481 ctcacctata ggtaaggttg atataaatga tgattttgag aaacatctgg acaggtttaa
      541 aagggctgta gacatagctt taaaaatgga gacctgttat attcgcatat tttccttcta
      601 catgaaaaaa gaggaactgg acatttacca acaaacggta ataaacagac taaagcagat
      661 gattgagata gctagggaaa atagtcttgt tcttctccat gaaaatgaag caaatattta
      721 tggagagacc agtgcaagat gccttaatct ttttaactcc ttgccataca ctaattttag
      781 agcggttttt gactcctcta actttgttgt agcaggagag gatgtcttta atgaaagttt
      841 cagtaaactt aaagattatg ttgaatatat acatgttaaa gattccaaaa agggttcagg
      901 agaaatggtt gtagcaggac agggtgatgg gcggatacgg gagatttttg atgaattgag
      961 ggataaagag ggaatgttca tttccctaga accacacctg acacatgcag gaaagtatag
     1021 aggattcacc ggtcctgagc tgtctgaaaa ggctttggaa gcattacaag ggattttgat
     1081 ggagcttaaa atagagttcg agtagatttt aacaggttat gataagaaga ctatactaag
     1141 aagactataa taagaagact atgataagaa gactgcgata agaagattat gatagcgggc
     1201 tatgataagt agattatggt aaaggaagtg aaagatttgg accgcaaaac aatggtatcg
     1261 gtagaacgcg taagggctgc cataaatcac caggaaccag ataggattcc acttgattta
     1321 ggcagcacaa aaatgacagg tatcagtatt aaagcatata aaaacttcat caaatataaa
     1381 ggatggcagg aactggatga aaaccctgaa atactagacc aggtacagca gctagctctt
     1441 gtacgtgaag gcgtattgga aaaacttgga gtggatacca gaggtcttat accatctgca
     1501 ccatcaaact gtaaaacgga atatagggaa gttggtgact atatagagtt tactgatgag
     1561 tggggtgttg gttggaaaaa acccatcacc ggaggctatt acttcgatat gacatcccat
     1621 tctatgtcag gagaaatgaa tctatcacaa cttgatgatt ttacatggcc cgacccactg
     1681 gacgaaacac gaacagctca gtttcccact cgcatagaag aaatcaaaaa gcatggagat
     1741 tatgctttcc ttatgcatgg agtcagtgcg ggtatattgg aaatggcctt gaggctaaga
     1801 ggctttgaac agttttttat ggatttgatt cttgaccccg ggcttgtttg tggaatactc
     1861 gacaggcttg tggagataaa gatggcttat tggggaaaag ccttggaaaa ggtagagaaa
     1921 gatgtccttg tagctatcga ggctgatgat cttggaaccc agaatagcct gttaatttca
     1981 cctgatatgt accggaaatt tgtaaagcca agacacaaag agcttttttc tttcataaag
     2041 gataaagttc cgggcataaa agtttttcta cactcatgcg gagcaataaa acctctcata
     2101 ccggatttaa tagaagccgg ggttgatatt ttgaaccctg ttcaggtgtc tgcaagtaac
     2161 atgaatacaa aagatttgaa gaaagaattt ggagatgttc taactttctg gggtggaggc
     2221 atagatacac aaaaagtttt gccttttggt tcgcctgatc aggtaaaaga ggaagtaaaa
     2281 cgcaggattg atgatctggc tcctggcgga gggtttgttt tcagcacaat acataatatt
     2341 caagcggatg ttccgccaca aaatattgaa gctatgtttg aagttctaga acagtgtgga
     2401 atttattaaa aataagtgtt gtatggcact gagaagggag agatattatg tcaataaaaa
     2461 cagcgatttt aggttatggg aggagtggta gcacccttca tgcagatcct attgaaagac
     2521 tacctgagtt tgacttaacg gcagtatgcg acatagaccc tgaagctcgc agcaaagctt
     2581 ataataggtt taagtgcaaa atatatgaag attataaaca gatgcttaaa gatgaagagc
     2641 ttgatttggt agttattgta accagaagtg accagcattg ccaaatgaca tgcgactgtc
     2701 ttgaagccgg taagaatgta cttgttacaa agccgtgggc ccttaatata aatgaagctc
     2761 aaagaatggc agcaacagca gaaaaaagcg ggaaactgct tatgccatgg ttgcctgcaa
     2821 gatggggatg tgatttgctt agacttaggg aacttattga atcaggagtt atcgggaaag
     2881 tattccaaat aaggaggagc gaattttctt ttggaataag gcatgattgg cagacagaga
     2941 gaaaatatgg gggaggatat cttcttaact ggggtcctca tttagttgat caaccactgc
     3001 agcttgcagg aaaacctgta aaatctgttt atggtgagtt gaaacagata attaatcccg
     3061 gtgatgtgga ggatgtattc tttgcagtta taaagactga ggatgacatt actgtagttt
     3121 cagaatacaa tattggttca gacaggcttc ctaattgggt tatacaggga gatagaggaa
     3181 caatatatgt aaaagagaca gaaatagaga tacataaggt taactacccg gaatcttttg
     3241 atcccaaatc ttatagaaat cctgtaacgg tagaagtaat tagagatgat gcaaaaggaa
     3301 caaacatggt aactatgggt aatagatatg gtgattctat ggttatctat cctcacatag
     3361 ctaaagctat gcgaggagaa gagccttata tggcttctct tgaaagcgca ttgactctta
     3421 ccaaactgct tgatgctata cgaaagtcaa gtgaaacagg acaggtagtt tctgttttca
     3481 gcactgcagt caggtgaaag atatttttac ggttacaatg gagttagttc tcatcgatta
     3541 tattcatgag gttctattgt accatgtttt tttgtacttt taggacgtaa tcatttgtgg
     3601 tatactagga cattatcata aattgatcag aaatgttaaa aaatttactc taaatatatg
     3661 ataaaaaatt taccctaaat atattgacag ttcaaaaaat atatgctaca cttctatcat
     3721 agttaggaaa tagttttacg aaaatggttt ccttaaaata ttatatcaca tctatgtaaa
     3781 aaactataaa tgataaaata aatgtatgaa ggggacaaaa ttgactatga atattaagga
     3841 tattgcatca ctcgcaaatg tttctgtctc cactgtttct aaaataatta ataaaaagga
     3901 tgatgcaata agtgatgaaa caagaaaaaa ggttcttgaa ataattaaaa agtatcagta
     3961 tgagccttac tcggaaatca aaaaaaaaca aaatccacat tcaggattaa taggaatact
     4021 cattcctcct aatgcgaatg aacaactgtt tgaacttgta agctgcatag agcaagacgc
     4081 atctagaaat ggatatagca tagttttaaa aattagtgat tttcaagata gcatgagcaa
     4141 aaggagcata caggttatca tcggcaaaaa agcagatgga ataattgttt atactgataa
     4201 taaaaaagac gaaaccatat attcattatt tcaggaccag gatattccag tagttttgtt
     4261 aaataaaact aatatacata tgcagcaaaa tattgtttct gtcttttgtg agaaaaaaca
     4321 gataggctta atggctgcta actaccttat aagcaaaggg catcataata taggttgtat
     4381 attatcttca gatttgcagc taagcgataa tgaaatctat gaagggtata tacaggcact
     4441 ctataatgta gatattctat ctgatgaagc atatgttatc attgattcag acccacaaaa
     4501 aactatagat tcagtaaaaa gtttgttaaa tatgaatgtt acagcttttt tatgtgagga
     4561 cagcaaacag gcattggcac tatatggaat gcttcagcag tttggtcttt ccgttccaag
     4621 ggatgtatcc attatcagtg cgggtgattc agcaatttcc gaaattatga caccaaagct
     4681 cactgcaata agatatccga tattagaagt tagtaatacc gctattaatc acttaataaa
     4741 tttaattgag aataaaaaga agccaaaaga gttaattact gatgtggaac caattttaat
     4801 tgaacgtgat agtgttgctg caccaaatga aaaaaaaggt caaaggaaaa aaatcattgt
     4861 tattggtagt atgaacatgg atattaacat ttatactgac gattttcctg aatacggaaa
     4921 agtattggta tcttccagaa ttgcattaat tccaggaggg aaaggtgcaa accaggcggt
     4981 tggagtagga aagctgggag gacaggctta tgcaataggc aggcttggaa atgataacga
     5041 tgggaaagaa atatatacaa gtcttgctaa taattatgtg aaaacagatg gaatcatatt
     5101 tgatacattg atgccaacag gtaaggcata tgtaagtgta gcaaaagatg gagaaagtgg
     5161 tattgtactc tatcagggcg caaatagccg gttttatgcc gaccaaattg aaaaattcaa
     5221 gcatttattt gaagatgcag agtattgttt aatatccaca gaaataccat ataaaactgt
     5281 gaaatatgca ataaagacat gcaataagag gaatgtaaaa gtaattttaa aaccatctat
     5341 gatacagaat atagatattg aattattaag gcatattgat tactttattc ctaatgaaaa
     5401 tgaacttaat caactaattc ctggagagaa tacagtttat gaaaaggcag attattgctt
     5461 taagcaagga gttaaaaata ttattgttac ccttggcgca aagggatgct acttaaaaaa
     5521 taaggattat gaaatgcact ttccagctgc tgattttact ccagtagata caactggtgc
     5581 tgctgatgca tttatcagtg ctcttgcagt atatttaagt gaaggtaaca gcattactca
     5641 atctatacgt ttcgcaactt atgcagcagg tattagtatt actagagaag gcgttcaacc
     5701 agcattaccg gatagaacag cattggatat ttattttgat aaaatagaac catccgaaaa
     5761 ataaagcaat tagataaaat aattaaaaat taattagtta taattattaa tcacctcaag
     5821 aggagcaagc ctatgaaaaa aattttagtt gtaggaagtt taaatatcga ttttgtcatt
     5881 aatgtagatg aaatgccaaa gactggagaa actgtacttg gtaataatct tgaattcatt
     5941 cctggtggta aaggagctaa tcaagcctat gcagcaggta aacttggagc tagtattgca
     6001 atgataggtg ctgttggtcg ggatatttat ggaacaattt tagttgataa tcttacttca
     6061 gttggtgtag atactacagc aattgccaga cttgataacc agaaaacagg ttcagctttt
     6121 atagtggtaa acaaacaggg acacaatagg atcattgtta tatcaggtgc taataaatgc
     6181 atatcaatag atattattaa tcgacatata aatatgattc aggaatcgga cattataatt
     6241 atgcagcttg aaataccaat ggatgttgta atttatgttg ccaaaattgc aaagcaaatg
     6301 ggcaagctgg ttattttgga tccagctccc gcacctgaat ttttacccga tgagctaatt
     6361 caaaatgttg atatattaaa accaaatgag actgaacttg ccagacttac aggttgcagt
     6421 atatatacaa ttgaagatat tgtgtctgga gcaaataaat tggttaatcg tggtgttcca
     6481 accgtaattg taactttagg agaaagaggc gcagtcatgg tgaatagaga tggctgggta
     6541 cataaaacag tcagttcagt aaaagcagtt gacacaactg ctgcaggaga tggttttata
     6601 gctgctctag cactgggatt aacccgagaa atgagtatag atgaggcgat tgatttcgga
     6661 aactctgtgt ccacattagt ggtaacacga aagggagccc agacttccat tcctactatg
     6721 gatgaagtaa aacttttgct taaacatata taatttaatg acgtttaagg catgcggaag
     6781 ttaataactt ttgcgatgca ttaatataaa cgtgtaaaaa taaattttaa tttaaaaaag
     6841 gagtgaggta aatgaaaaag gtattatcat taattcttgt agcattatta attactggca
     6901 ttctaagtgg atgtgttgtt aaatcaaacg aaacaaaaat accagatgct gcatctaatg
     6961 aaacaaaagc atctgcggag gctaaaaagc tcttaccaaa accaacaact tttacaataa
     7021 tgactttcga gcaccctagt acaacagtga acccttatgg tatcagatgg caggaggtat
     7081 ttgacaagac aaatgttaaa ctggagtttg atataacccc tcaaggtgat ttcaatgcaa
     7141 agttagcaac tgtactggct tcaggtgtta tctcagatat tacctgggtt aagcagaaca
     7201 acacaacaga atatggaaag aaacttttta tggatttaac tgacaaactg gaaacagaac
     7261 tacctaatta ctggaggaaa gttaaagatg atattaattt tctaaagacc aaggttgaag
     7321 gaagagtata tagtacatat tttcttcaaa atgataatta tccaggcggg cttgttccag
     7381 ttatacgcta tgaccttctg gaaaaacaca atctccctat gcctgaaact tttgagcaat
     7441 ggtttgaggt catgaaacag cttaagaacc tttatcccga cagtactcct tattcaggga
     7501 gaggtgggcg taatactcct ttaaatcttg agtatgcaat gggagcacag agaaatctaa
     7561 actatgatct tgttaagcaa cgttatgtat atggggtgtt ggagcctgag tacagaaaaa
     7621 ttcttgaatt cctaaataag tgttatgctg aaggaatttt agacaatgga tatatgaata
     7681 ccaatgctaa tacttgggca gaaggcgctg caaataacaa gatctttttc tggtatgata
     7741 atggcggatt tgcaaccagc cagactttag cactgaggca gaacaaccca aatgccctaa
     7801 tgcagattat gccattgatg gaaaatgctc aaggtgaaaa aagaggtcag atatacgcca
     7861 aacactggta caatgaacaa ctggtattta gcaaaaacac atcaaaacct gaagaactcc
     7921 ttaagtttat taactggatg tacagcgatg aagcattgct aattaacaat cttggtaaag
     7981 aaggtatcac ttacaatgta cttccaaacg gagaagttga acttccaaag gaacttgttg
     8041 ataagtacaa agtatccaca agtccggatg atgcctggca atcagacctt ggagttggtt
     8101 atcttaatgt tacgcctttt gttggatata caccattatt aggtaaagca cttggaacat
     8161 ttaatgaagg tttttacgat gttctattac cagatcttaa agccggatac tataaagaac
     8221 agtgggttgc tgtggtatta gatcctgaag taaagaaatc aattaatgaa cttgaaaagc
     8281 agattaattc acttattaat aatcaaattc caaaattcat tgatggaaca agacccctaa
     8341 gtgagtttga tcagtttgtt gcagaagtta aaaaactcgg tgcggaagat gttttaaaag
     8401 catacaacga tgcagtggct gctctgaaat aagcatgcat taatgtaaca ttaatataaa
     8461 ataaattatt tactaaatca gtaaagcact ataaagtatt gagggcatac cacaagagtg
     8521 tgtatccaat tatcagtggt ttgtcctctc accatataag ataggagtgg gctatgtgat
     8581 aaataattat actgataaac cattagttaa attaccttca gaatgtaaac ttattttcaa
     8641 tgctatgaaa ggctggagct attctcatca tcccttcatt acatttttta aaggtaaatt
     8701 ccacgcaata tggtctaatg gacgtgttaa tgaagatgat tgtggtcaaa gggttatgca
     8761 tgctacatca gaaaacttta tagattggtc tgtacctgaa cctcttgtga actctattat
     8821 gggtattgga tcagaacttg ttttgactgc atgtggtttt tatcagcata atggtgtttt
     8881 aaatgcctac tttgggcatt atgaatatga tccttcatca cttcaggcaa agaatttaag
     8941 acctaaagga gatattgctc acataaatgt agggttgagc gttgtaagca caaccgatgg
     9001 tgttaattgg agtgcaccaa gagaattagg aatacctatg ggagcgaatt ttggacctca
     9061 accaacaaaa tcaggcagac ttattatttc aggaagtata gtatatccat acagtgatga
     9121 tataagcggg acaggaaaat tcaccttaac cggaatatat gagagtatag ataatagagt
     9181 gagagatgat tctgaagcaa tagaagttac gataaaaaga aaacaatggc gggatgccag
     9241 gttaatttgt gaagggtctt tttttcagac tgatgatgga gtaatccata tgatgttgag
     9301 aagtaataca cccaggcttt ggtgtactga aagtactgat gacggaacaa aatggtcaga
     9361 gccctttcct actgagtttt cagatgatca tgcaaaattc cactttggca gattacctga
     9421 tggacgtttc tattatgtag gtaatcctat accaagaagc ggaagaaacc cacttgtact
     9481 ttgtttgtca gaagatggaa taaactttaa tatgcattat attctaaggg atgaaccata
     9541 caagcaacag tttgaaggca tgtacaaggg gggactatat ggctatcctt gcacattgat
     9601 acatgatgga tatatgtatg tcatttactc aaaacgtaag gaaagcgtag aagcaacacg
     9661 ttttcaatta agtaaaatat aggtaatatc gggaggaaac ctcaatgtgg gtgaaaagaa
     9721 gagtattatt gatacctgtt attatatgtg taagcatatt aattacatct atagcaataa
     9781 ttgatgcagc aagtttacaa cagaatatca acagcctgaa atcattgaat gctgacaact
     9841 taagcaatgt atctattcaa aatgaattct tttctaaggt atttagtgaa tatccagaga
     9901 cgcttgctgg cgaaaatatt gaaatagatt tactaaaccc cactgatggt actgccaaga
     9961 tagaaaataa tattggagga aaccctaaag cagctttaat ttgggagcat ttaactaatg
    10021 aatataaatg gaaatttaaa gtgaacgaag caggacagta tgtaataaca ataaattact
    10081 atattttagg aggatctggt aataatgcga ttcgtagtct taaaatagat ggtaaaactc
    10141 cattttcgga agcagataat attgtatttt atcatgattg ggaagataaa ggggaaatta
    10201 taaaaaacag tattggagat gaagttcgtc caagtgtaaa agcaaaaaaa agctggcagc
    10261 atgtaatcat taaagatgga agcggatttt attcaatacc tttagtgttt tattttgaag
    10321 aaggtgaaca tgagctttca atgaattatg tttcacagga tatggcaata tctgatttag
    10381 tctttgtacc atataaagaa ataaaaagct atgaaaaagt tagcaaaagt cataacagtg
    10441 agacaaaagc gaaagaaatt atgctaacat ttgaagcgga agatacagta attactaaaa
    10501 atgatgcaac cttaagaatt gagagtaatg gagatccggc aactacgcca aaggcatttg
    10561 gttatagggt ttttaatacc ataggaggat ggcgctggag aagaggcaac caatctataa
    10621 cctttgaatt cgaagtaccg gaagatggtc tttacaagat cggatttcgt tttcttcaaa
    10681 catggaatga tgggcttcct tcatatagaa caattgcaat tgatgatgaa gttcccttta
    10741 aagaacttct ggcttaccgc ttcagttata acagctcatg ggaaacagaa gttttaggag
    10801 ggaaaaatcc atatcttttt gaactgaaaa aaggcagaca cagtattaca ttaacagtta
    10861 caatgggtga attaacgcct attatacaat ccttatatgg tgatatattg ttaatatcag
    10921 atatgcttca ggaaattgtc aaactgacag gaaatgctcc agacccaaac tatgattatc
    10981 aattttttaa gtatattcct acattagaga atgatttata tacattaatt gatagtcttg
    11041 attataaata tcagatgatc aataatatta ctacaaaatc ttctaccatg agcggtaact
    11101 ttaaatccat aaaggaacag ttgataagca tggttaaaaa tccgttttcc atagcacgca
    11161 aattaaatca gataactcaa gcccaatcaa gtctcggatc ctggtattta gagctgcaaa
    11221 atcagcctct attgttagat aaatttagtg ttgcaactcc tgatatagag ataaaataca
    11281 ggaaatccaa tatttttgaa agaatgttga ctgaatttca aaaccttcgt ctatcatttg
    11341 taaaagatta taataacatg gcaagtattg ttaaggatga tgtagaagtc aaatctgtta
    11401 tagatgtgtg gattgcaaga ggaactgaat gggcagaaat agtaaaggaa atggctgatg
    11461 aaaactttac ccctgaaacc ggaattttaa ttaatatgaa tgttgtacca tcatcacagc
    11521 tcaataccgg aagtgctaat gctattatgc tgtcaattat atcaggtagt gctcctgatg
    11581 tggcaatggg tgttgactca ggctcaccgg ttgaatttgc aattcgtgat gctgttgtag
    11641 atgtatctca attttctgac tatgaggaga ttgaaaagag attcttaaag aatataatga
    11701 ttccttacaa gtacaaagag ggagtatatg caattcctga aacaatggat tttacctgcc
    11761 tttattacag aaaagatatt cttgagaagt acaatatctt aatacctgat acacgctacg
    11821 aactatatca ttatttactt cccgaacttt accaaaatgg aatgtcttat tatcagcctc
    11881 atgactttac atcattttta cttcaaaatg gaggttctta ttatacagaa gatggattat
    11941 attcggcact tgatactccg aaggctttcc aggctttcag ggaatatact gaaatgttta
    12001 cccactatag tgtaccggtt aatgcaaact tttttaaccg catgcgtaca ggtgaaatgc
    12061 caataggaat tggcggatat ggcctttaca tgcaactctc cactgcagcc ccggagcttg
    12121 ttgggcgttg gggtattgct cctatacctg gtgttttaga aggagatggc tctgttaacc
    12181 gttctaatgc aggtattgca gctcagtgtg atattattct gcagcaaagc ggcaaaaaga
    12241 aagaagcatg ggaatttata aaatggtgga ccagcaaaga agtacagata caatatgctc
    12301 gtgaattgga agcacttgta ggaatagaag ctcgctggaa taccgctaat gcagaagcat
    12361 ttgaatcact tgaatggcgt caggaggatc tggatgtctt caatgaacag tggaagtggg
    12421 cgaatgaaac gccagttgtg cttggaggtt attttacagg aagatatata aataatgcct
    12481 ttaatagtgt agttgtcgca ggaaataaaa cacctcgaga tgctcttgag gatgcagtaa
    12541 gagaaataaa caaggaatta aaaatgaagc aggaagaata tggggtgttc agagatgaaa
    12601 actgaggaag taattataaa tacacatatt gacaaaaaaa tatctcaaac aaaatttgga
    12661 gaaataaaaa tgtttatcca taagcattgg gttggatata ttatgcttgt gccatttttt
    12721 attctcttta ttatatttgt agttttacct gttattatag cacttctgtt aagttttact
    12781 gattacaaca tgcttcaacc tccaaaattt gtcgggctaa aaaactatga actattattt
    12841 cttgatgaca aaattttctt aactgctcta gggaatacat tcctttttgc cttcattgta
    12901 ggtcctatcg gctatttaat gagctttatt gctgcctggg taattaacca attaaaactg
    12961 agaaatgcat ttgcacttgc tttctatgca ccttccataa cttccggagt tgcaatgagc
    13021 gttgtctggc tttacttttt ctccccagac agatatggat tactgaataa cctttttatt
    13081 aatgcaggtt tgatcgataa tcccatattg tggacacaag atcccaacta tataatgcct
    13141 gttataattg ttatatcaat atggatgagt ttggggaatg gatttttggt ttttctggcg
    13201 ggacttcaaa acataaataa ggaacactac gaggcagctg caatagatgg tgtagaaaac
    13261 agatatcagc aattaaaata tattacacta ccaatgatga aaccacagct attatttggt
    13321 gcaattagtt caattacaag ttcatttggt gtttttgata ttgcagtatc agttgcaggc
    13381 ttgccaagtc caaactatgc gggacatact attgtagcac acttatatga ctatgcattt
    13441 atacgtttcc aaatgggata ttcttcagct attgcaactg ttctttttat tataacttat
    13501 tcatttgggc atataattat gcgattactg tcaacaaaag atgaatagag gggataatag
    13561 tatggttaaa tttaaaatga ctaaatcaaa aatgaatgtt ttaaaagtaa aaaaggaagg
    13621 aaagtggaca tttgtatata tttttatgat agcacttgta gcttttactt ctctaccatt
    13681 ggtatactta atatctacag cattcaaacc gctaaatgag ttgtttttat ttcctccaag
    13741 attttttgta aaagaaccga cattgaaaaa tttcactgat cttatgcttt cattgtcaag
    13801 ttctacagta ccttttacca gatatattct taatagtctg tatgtaactg ttattacggt
    13861 aatattaaca gtgtttgtca gcactcttgg tgcatacagc cttgttaaac ataaccctcc
    13921 gggaacaagg aaacttttta aatttataat tgctacaatg atgttctcac cttatgtaac
    13981 gcagatcccc agatatcttg tagttaattc gattggattg gtaaataaaa ttgctgcttt
    14041 aataatacct aatattgcgg tatcatacaa tttttttcta ataaagcagt tcgtggaaca
    14101 gtttccaaat gaattgctgg aagcagctcg tatagatggt gcaggtgaat tttatacata
    14161 ttggaaaatt gttatgccgg tactaactcc agcttggtcg actttaattg tgtttagtta
    14221 tgtaagttcc tggaatgatt atttttctcc attaatttat ataaacaatc aggcgctaaa
    14281 aacacttcca ctggcactta gaacaatttc cggcggtgga gcaagtattg gtcgtgcagg
    14341 tgctgtagca gctgctacaa tgcttatgac aattccaaca gtagttgtat ttacttcaat
    14401 gcagaacaaa gtaatggaaa ctatggttca ttccggaatc aaaggataag ggggaaatga
    14461 tacttgaaaa ttagagtatt agttctaaca attatattta taatgatgtt tagtagtgca
    14521 gctatggcat atgaagtaga ttatattatt aatgatggcg agcatgtgcc tattccgcag
    14581 ccatatatat atagtaaaac tattaattcc ataaaatata atgatgaagc tgcaattaaa
    14641 tattttaacc agccggcgga tatttttatt gataaaaatg acatgatata tgttgtagat
    14701 acaaaaaata accgtattgt gaagcttaca tacgatggtg aagcaaaaat gatattcacc
    14761 gaagcaaatg gcaaaaaatt taatcaacct caaggcatat atgtagatga taactttgat
    14821 gtttatattg cagatacagg aaattcacgt attatacagc ttagtgcaga tggaagtttt
    14881 ataaaagagt ctttaaaacc tgaaagcggt atgctttccg atattgccat atatagccct
    14941 acaaaaattg caaagtctat aacaggtgga ttatatgttc tattgagtga aaatataatg
    15001 gttattgatg aaaataatag attccgggga tttattgggc aaacaaaaat tggatttgat
    15061 tttacagaat ggctgctaag aatagttgct tcagatgtac agaaaaaaag tattgcaaag
    15121 cgtttggctg caagttatga taatttttgt gtagatgaca agggattgat atatgcagta
    15181 agcagagata cagttgaagg tcaggttaaa attttaaatt ctattggtaa taacatatac
    15241 cgcaagtata catcagttga tgataatatt gacaccgtag cagattatat caaaaacttt
    15301 ttttcaggta atataatttc aaaatctttt cagtatggtg aaagcgtata tactaccaaa
    15361 tgggaaagac ctgtatttat cgatattgcc gttagttcgg atggaattat aacagctatt
    15421 caaaaacaga atggtaagat ataccagtac gatcaaacag gaaacttatt ggcagttttt
    15481 ggaggacttg gttctactca gggtaagttt gcaataccgt catctatagc tattgatagc
    15541 caaggaagat tgtacgcact ggatttaagt tatgggaaca tacaaatttt tgagcccact
    15601 gagtttataa aattggtgca gcaagcaacc attatgtata ataatggtaa ttatgacgag
    15661 gcaagtgcta tttgggaaga aataatgcgc tacgatgaaa catatccatt agcacattat
    15721 gggctggcaa gcactaatta caagctggga aattggaaag ctgcaatgga tggatatttc
    15781 aattcaaata acagagtaga atattcaaaa gcatttacag aatacagata taagttactc
    15841 aaaaataatt tctttattgt tgtattatta tcattggcga ttatttcact tctttacttt
    15901 attatagtaa gaatgtggag aaatactctt gtggtactag atagttttga aagctataag
    15961 ttgcaaaaac ttaatttttt gaatggactt gttatgggac ttggaatttt atatcgacca
    16021 ggtcgtgcac tggataacat taaaagtggc agaggaagat taaatgttaa atgtgcatcc
    16081 tttatttggg cggttgttgt tgctgtaaga ttcttcttca tatacaatgt ccactatcca
    16141 ctgcaggata ttgaaattca ggatgttaat tttttattgg aaattgtaaa aattacattt
    16201 cccgtacttt catgtataat agcagctttt ttgatttcat cacagtttaa tggggaatct
    16261 acattggagg aaaattttgt tgctatgaca tattgtatga ttccttatat aattgttaca
    16321 atatttgcaa ctttattttc acaaatacta tgtacacaag aaaagatgct gtttgccctt
    16381 tttgtaaatg gagtaacaat atggcagtgg tggctgataa taagggcagt taagagactt
    16441 aatgattata acataagaca cacaattttt gtctgtgtaa taactttctt tgctgtaata
    16501 ttaatttggt ttaccgggat atttgcatat acattggtcg cacgtatgat acaatttatt
    16561 gaagaaatat tgctggaagc acaattagta ttcttttaag ggggcattat atgaaaacat
    16621 tgaaacatat tatattgttt gttcttatta tagcagtatg ttttagttct gtgaattcct
    16681 ctggagcaga taatagtatt actaatagta ctactattag taatagttca gtatttgaat
    16741 ccgaccttcc ggatcaatat ttatttatct gtgaaaatga tagattttca ttgagctata
    16801 gagaggatat cacttcaatt gcacttactg ataagttaag tggaagaatg tggtattcgg
    16861 cagtaaatga agaattatat gatattaaaa attcatcaaa aacttggcag acttatatgc
    16921 agtcaatcct ttctattaaa tatgtgagca aaaaagatac aagaggcaac tttattaatg
    16981 attattctgc agcaaaagat aatgcagtat ttgcagagaa gcttgaaaat ggattgaaat
    17041 tacgcatttc ttttagagat tcggcaattg atttaactct taaaatcgaa cttgaggaag
    17101 atcatgtaaa attatctgtt cctatagacg agattaagga aaatggtgag tattctattg
    17161 tttctgtaga gctgcttccg ttttttggtg ctgctgcaaa ggatgctgat gggtatattg
    17221 tgtatccgga tggcagtggc gcgattagct attttaatga aacagataac aaacatcagt
    17281 ttacgaaaca atatactatt gatgtttatt ccttgcttga tcatgagaag atatttgatg
    17341 aaacaaatga agaaaattgt gcgatgctcc ctatttatgg aataaaaaat gagaagaatg
    17401 ctttccttgc tgtaattaca aaaggagatt caagtgctaa aattaatgtt aacaccagtg
    17461 ttaattcatc agcggtaaaa cttaatagag caagttttga gtttgtgtat cggaatcaat
    17521 accggattta tctttccaac atcataataa atggcaggag cagaacaaca aacccctacg
    17581 gagttaaagt tgataaagat atgatacaac ttgattgcga agttaagctt tttttcttaa
    17641 atgatgatgt agcaaattat agcggaatgg caaatgttta tagggattat ttgataaaag
    17701 agggattgtt aaagaaaagc aagcttcaag atgaggtagg acttgcacta aatgttttta
    17761 tgggaacaag agaagaaaaa acttttttaa agaaatttgt aaatatgaca acctttgacg
    17821 aagtaaatag aattgcatct gattatctta ataatgatat tgctaatttg caaattatgt
    17881 tgcggggatg gagtaaaaat ggatattaca accagcctaa tattgccaca ccggacaatc
    17941 acctgggagg ttctgcaggg ttaaagagac tggcagctct tgataaagaa aatgataaat
    18001 tatttttata tctggaaacg aatgtgaata gtgcatcaaa aagctcagga gggttctcaa
    18061 caggacgtga tgtagtatta caaggcaata agataccagt atcacataat aaaagtaaat
    18121 tcttaataaa tgcaaagaag atggaaagtt tatatgaaaa tgcacttata aaaatgtcag
    18181 ctaatttgcc tataggtgta gcgcttgcca aggcaggttc atttattact catgactaca
    18241 ataaaagctt tggactgcaa agaagcgata atattgaggt atggaaaaat atgatttcac
    18301 agacatatag taatgtagca gctcagggtg gaaatcttta tacattagct cttgttgaca
    18361 ggttatacga tatacccata gaatcctcca tgaaccaaat tacggatgag agtataccat
    18421 ggtatcaaat gatagtctat ggttcaatac cttatacttc agaacccgga aatctatcat
    18481 atgatttaga acttttaaaa ctgaaatgga ttgaatatgg atgcatgcca tattttgaag
    18541 taactgtaag aaatcccctt aatttaaaga ataccagtta caatacactg ttcacttctg
    18601 aaaacagtaa gtggagggaa gttattttag atatatataa tgaatggaaa tcaaagctag
    18661 aaccaataca aaatactttt atgatcaaac atgaaaaatt aactgaggat attacacaaa
    18721 ttacctacga aaatggatat caagtaataa taaatttttc cttttctaaa ataaattaca
    18781 ataatatgga aatacctgca agagattaca ttctggtagg aggtgcatat aattgaaacg
    18841 tataagaata aaatcagaaa caaaacaagc acttattgga ttattcttta taacaccttt
    18901 catgattgga acaatagttt gcttcctata tcctgttata gcttcttttt tgttaagctt
    18961 cggtgataca aacaaggaaa tggcaggttt tcatattaat attactggtt tccaaaatta
    19021 ttaccgcgca ttcatggaag atacatcttt tgtgccaatg ttgcttaatg tgcttaaaca
    19081 aacactcaca caaacaccat tgattgttat attttcgcta ttgattgcaa ttatgctcaa
    19141 caaagtaaag aaattaaagg gattcttcag ggttgttatg cttcttccat ttttgctagg
    19201 gacaggtgaa gtacttaatc atttgacagg acagggagta gataaacaga taatatctat
    19261 ttcaaattca aatatcatac cacgtgagtt tattctatac ctgggttcag atattgtgaa
    19321 attgcttgat actctttttg gaactatagt attggtcttg tggtccagcg gggtgcaaat
    19381 acttcttttc ctgtcgggaa tccagagcat aacaccttcg ctttatacat cggctcgtat
    19441 tgatggtgca aatgaatatg atatgttctg gaaaattaca cttccaatga tatcgccaat
    19501 tttgctatta aataccattt atactattgt aaattcattt acaagcaaat taaatccaat
    19561 cctggattat gtaaaacaat atgcatttgt taattcgcaa catggatatg ctgctgctat
    19621 ggggtggatt tatttcgcgt ttattctttt gataatatta attgttatac tggtgctggg
    19681 aaattatgcc aggtcaagtg gcgttaaggg gaggaaagaa taaatgaata ctggagaaac
    19741 aataataaat atgaactatt ttgtaaaaaa acatgacctg aaacatgacc tgacaagtca
    19801 tcgttggata cttaaaataa agaagtatct tccaagagta atactttata cttttttact
    19861 agctatttca tttatattta tttatccttt tttatacatg attgtaacta gtctaaaaag
    19921 taatgaggat ctaaacaatt ttatagttgt atggattcca cgtactttgc atttcaataa
    19981 ctatttactg gctgcaaagc taatgaactt ttcagtcact tttaagaata gtgcaattac
    20041 tacagcttta gcaactattg ggcagttgat ttcatgttca atggcaggtt atggaatggc
    20101 cagatttaag ttcccgggga aaagactagc tttttttata gtaatattag ctcttattgt
    20161 acctgcccag gcaattatag taccgcaata tttgctttat tccaagttag gttggcttaa
    20221 tacctatttc ccacttacgg tacctgcttt tttgggattt ggatttaagg gtgcacttta
    20281 catttttatt ttcagacagt tttacctggg gttgccaaaa gaattggaag aagcagcagg
    20341 tgtagacgga tgcggatttt tacgtacata tatcaaaatt gttttcccga tagctcgatc
    20401 agcttatatg gttgttttgg tattagcatt agtttggcac tggagtaatt attttgaacc
    20461 agcgattttt gttagccgta caaacctaag aatgttatct tccggaatca ataatattgc
    20521 tgatacactt agactgccac ccgaaactct ggtctcaatg tacaatatat cagatgaaaa
    20581 tacactaaac acagcagttt taatggcggg tacattcatt gttgttttac caatacttat
    20641 tatgtttggt atattgcaaa agcagtttat tcagggtatt gaacgtacag ggttaacggg
    20701 tgaataaatt taaaaaacgt tctattacaa aaatatgcat tccttgcggg tcagcaaaaa
    20761 ttaccctcca gaaatgtaat ttccattggc aacataacaa agtaatatat tttattttaa
    20821 aaaaaataaa tgaaaggaag aataataata tggatactaa aaagaaaaaa gttttattcg
    20881 atactgatat tggatccgat attgatgatg cagtagcact tgcatacctt cttagacaac
    20941 ctatgtgtga gcttgttggg attactacag taacagggga tactgtaaaa cgtgcacagt
    21001 tggcaagtgt gttgtgcctt gcagcaggga aaggggatgt ccctatatat ccgggatgca
    21061 gaatgccatt acttgataat gaactgcaac ctcatgtacc acaagcagat gttctgcctt
    21121 tatacaatca tcagaaagaa tttccaatgc acaggcatat acaatttatg attgatacca
    21181 taagagctaa cccaggagag atatcactat tggcagtagg acctatgacc aatataggac
    21241 tattatttgc tacagatcct gaaattcccg gtttactaaa agagcttgtt ctgatgtgtg
    21301 gagtttttac aaatacacta ccacgggtag gtccaagaga atggaatgcg tatggtgatt
    21361 ctcattcgac tgctattatt tataatacaa gacctgctgt tcatcgctcc ataggactgg
    21421 acgtaacctg ccaggttacc atgggctcaa atgaagtcag agagagattc aatgttgatt
    21481 tactaaaaat tgtcttaaca atggccgaga tttggtttaa ccgccgctca attataacat
    21541 tccatgatcc gttagctgca tcaactttgt ttgatgaaag tatatgtggt tttgaacgtg
    21601 gaaaagttga tgttgaattg ctaagcgatc gtgtacgtgg tatgacctat tttaaagctg
    21661 ataaggaaaa cggtcctcat gaagtagctt taagagtaaa taaggaaaaa ttttttgaac
    21721 attattttag cttattttaa gcaaaatttt taaatcacat ttataagcca acataatttt
    21781 ggaggtgtaa atatgcaatt aactattaac aaaaaatatc tgcttcttcc tgtaagttcg
    21841 ggagctgtaa aaaaagtact tttattttat cataataaaa agcttgttta tgaactatca
    21901 acaaggattg actatttagc tcctgaatat tttttcccgg ttgatatgca tccattccag
    21961 ggtttaactc ttgatatctt atgcaatcct gatatgaagc ttgatttgca tatgactgat
    22021 atcataccgg aactgccgga atcaacgaaa cgctatagac cttttttaca ttttacagtt
    22081 aatcaaggat ggcataatga tccaaatggg cttatacaga taaatggaga atatcatatg
    22141 ttttatcagt ataaccctgc agatagcgaa tgggggaata tgcactgggg tcatgctgtt
    22201 agtaaggatt tgtttcactg gcaggaacaa gatgttgcac tctatccaga tgaaatgggt
    22261 actatgtttt cagggagtgc aattattgat agtaaaaaca gtagtggtct ctcaaaaaat
    22321 ggtgagcccg ccatactgct tttctataca gctgcaggta atacatcaga gcattcaaaa
    22381 tacaaactat tcactcaatg tattgcttat tctacggata gaggaaaaag ttttgtaaaa
    22441 tatgagcata atccagttgt acaacatatt caagcaaata atcgtgatcc aaaggtaatt
    22501 ttttcaactg aattaaataa atatgttatg gctcttttcc ttgaaagtca tcaatattgc
    22561 ctgcttgttt cagatgacct gcttaaatgg gaaaaactcc aggatataac tttaaatgat
    22621 gatagtgaat gtccagattt atttcctctg gctgtaaatg gtaataaaga aaatattaaa
    22681 tgggttttct gtggcgcatc tgatttttat ttggttggaa gtttcatgaa gggtaagttt
    22741 gtcgctgaac aggagccgaa acgattattc catggaagag tttcttatgc tgcccagaca
    22801 ttttcggatg tattagatgg tagacgcata agaatagcat gggatagatt aaaaattcca
    22861 aattctccat ttaacagtca gatgggagtt ccatgtcagt tgaatcttat agaggaagat
    22921 aaccaatatt atctttgcgc acaaccagta tctgaaattg cttcattgta taagaatgta
    22981 catatagaag aaaatcttct tgttgaaaca aatgaaccgt ttaaactaga ccttcaaaat
    23041 aaagcatatg atataagcct taccatttca gaaagacttg agaataattt ctctttgaca
    23101 attttcggac agataatatc atataacttc gattcaaacc agattatctt caaagataat
    23161 aaaattcctg cgaaaatagg gaaaggtcct tttacaatga gaatgattgt agataaattg
    23221 agtatagaga tattcaacga tttaggacgt gtagctatat gcaatagttg catatgcgat
    23281 tataacttaa ataatatgga ggtgcattct gatagcaaat tattgataga taagcttttg
    23341 atttcagaaa ttgaaatata attaaaatac tggtcataaa acttaatata agaacttaat
    23401 accatagaaa taagggcgct atttggatta attgcagcaa ttatccaaaa tagttcacta
    23461 ttgattgatt ataccaaatg tttttcacat tctcctatat ataagtaaaa tttttaaaag
    23521 tagtattttt tagataagga gagcttatat gaaaggatat aatgcttgga attatgcacc
    23581 ttacattccg gctggagaaa gaacaggtac atcagttcca tatatatgcc gtttggcgcc
    23641 ttcctataac aaagtagagg tagaatggtt ccacaataat tatgaaggag tgcatcgaat
    23701 cttttattcc aaagtcaact ccgggtcatg gcaggaacag ctaatttcag aaagaacctt
    23761 taccattgaa ggtttggaac aagatatgga atacatgtta tatattaaag cggataacgg
    23821 acctcaaagc atcgtccgtg ttatacgaac aggttttgta cctggagcag tggttaacta
    23881 tcttcatcct aaagataatc aatatagctt ttcagggcaa tatctatgca gtccatcgct
    23941 agttaaatta ccaagtggtg tattacttgc atcaatggat gtttatggaa gggctgcccc
    24001 tcagaacctg acacttattt tccgttctaa agacggtggt agttcatggc agtatgtgac
    24061 agacattttc ccttgctttt ggggtaaaat gtttttacat aaagatgctt tatatatgct
    24121 tggtgtttct aatgagtatg gagatttatt aatcggacgg tctgatgatg aaggcaatac
    24181 atggacagca cctactgtta ttatgagagg ttccagcaat ataaaggaaa atggcaatca
    24241 taaggcacct gtagcggttc ttcaaagtca tggaaggctt tggacaggct ctgagtttgg
    24301 atgttggcat aagaagcaat tcaaaagcag tttgtattca attgatgaaa atgatgattt
    24361 aatgcaggct aataactgga cttgtaccgg gtttttagct cataatgata catggccggg
    24421 agcggaacag gagtgtgccg gaggaattga aggaaatgta gtagagtctc ccaaaggtga
    24481 aatatttaat atactaagat attcgaaaag taaagcttta atgttaaaag ccaatcctga
    24541 taatccagaa gaaggtctga ctttttataa atttattgaa tttccattgg cacatactaa
    24601 atttgaaatc caaaggcatg ataatggtat ttattatgct gtgggaaata agtttcctgg
    24661 acgaacagta ttgtcagttt atacctcaga agacttagaa cattggaaat ttaaatgtga
    24721 tgtaatgaat tactatcaaa tggatataaa agaagttggc tttcaatacc cttcattcta
    24781 ttttgatgaa gatgatttat tagtgcttag tcgcactgct tttaataatg ctgcaaattt
    24841 tcatgatagt aactatatta ctttccatag ggtaaagtta tgtgttcatc agcagtacac
    24901 cagatgatag ttattcgctg ctaaagtgaa taaacgaata agatgagtgg acaaagtaaa
    24961 tgcaaccttt tataagtcaa aagaaaatat ttatgaggta tgaggaagag aatatctggt
    25021 taacacagaa aatgatggct atgttgtacg atgtgtcagt ttccgctgtc aatcagcata
    25081 ttaaaaaggt ttttgatgac ggtgaattag agctggattc agttattaaa aaatacttaa
    25141 taactgctgc tgacggcaag caatacgaca caaatcacta taaccttcaa atgattattg
    25201 ctgtgggctt taaagtaaat aatcagagag ccgtccggtt taatgtttgg gcaaatcaaa
    25261 ttgcagagca atatacgatt aaaggttggg ttatggattc cgaacgtctt aaaaatggtg
    25321 gtagtatcct tacggattgg aactaattgt atcagcgtat ctcgaccttg cggaaaacag
    25381 agtaagacgg catatcccga tgacaatgga agattgggca aagcggcttg atattttctt
    25441 gcaggcggac gaacatgcaa tcctaaagga tgtagggcta tttacggcag agattgctaa
    25501 atcttatatc ggaagatttg tatctgtaag gagctgcttt gcgtgctcaa tccttgtgtt
    25561 taaatatagc tcataggtga tagaccaaga ttttctttaa acttatgtct gaaatgatga
    25621 tagctgtatc ctgataattc ggcaagagtt tgaaggctta tattctgatt ataattttca
    25681 cggataaatt tcttaatgta tcccatctca ttgtgctcca taattttgct ttcttccaac
    25741 ctataaagct ctaccaacat ttctgtaaca agtaaatcca gctttaactc ataaaaattt
    25801 tgtttagatg caaattcatt ccgaatttct tcaagaatat taagaattga tcttgatgct
    25861 ccatcaaagt atataccctc cctaatgcta aaggaatagc agcattacat attgaaaacc
    25921 cgacaaacaa tacgggggta tcattataat gcctttcatc atgtatcgta tttggtgaaa
    25981 taactgaaaa ggaatttttt gaatattcgc attcctgttg acctattgtt gtctgtcctg
    26041 aaccagataa atagtaaaca agttcaaaac aattatgttt gtgaaaattt atatattctg
    26101 catttttacg gatgatagag taaataaatt cgaatttgca gttcaagcaa attacacccc
    26161 caaattgcat aggaaatatt ttcttcctat aagcccttac cctttaggat agttggcagt
    26221 tgatatagag caaagcaagt ttgcttttag tatattttag cataatttat atttgtatac
    26281 tatagattga atcagcagta cactaggagt ccgctttgat agctcagact ttccgagcaa
    26341 actcctagat gataattatt tgctattata acgaataaaa ttcattatat cttattaata
    26401 cattgaattt tattcgatga catagtataa ttgtcttata tcggatatat aacacaaata
    26461 taattcaaca aggaacaaat tggcatgcta gtatcgatat aatcgcacta aaatttaatg
    26521 atgtagtttt tttatgtgtt atatgtaata tatagaaaaa tattcttact gggggagtta
    26581 aaatgtatat taatccttac agcagtgctg cagaaggcaa aggcgcatgg atgaaatcca
    26641 attttcatac ccatgcagga acaggaccgg atacctgtgg cgcatatgat ttggaagaag
    26701 ttgtaaaaca ctataaagca gctggttata tgatcctttg tatatcaaat catgaccttt
    26761 ttactgatac ctctcacctg aaaggaaaat atgggattgg attgattaat ggttttgaat
    26821 atagtaaaaa tctacatatg ctttgtattg gctgcaaaag tctaatagaa ggggagcatc
    26881 aagcagcagt taatgaatgt gttaatcagg aagggtttgt aatactttgc catcctaatt
    26941 ggcagcataa ggagtattgg ccgtggggaa agatagatgc attaaaagga tatgcaggta
    27001 ttgaggtttt taatggcgta atcggccgtt tgactggtac aggtcttgca gaagatacat
    27061 gggattatct gctttccggc ggcaaacttg tttggggatt tgggaatgat gattttcata
    27121 ggtggcatga tttaaataaa agctggacca tgatttacag tgaatctaat gaagaaaatg
    27181 atgttaaatc agctattcgt agcgggtctt tctatttatc aacaggcctt tttcttaatg
    27241 agattacttt tgacaacaac ataataaagc taagagcaag tgagaatgct tgctatccaa
    27301 agaattatca atacacgttt attggtaaaa gtggagaaat gctccacagg caggaaggag
    27361 aatttggtga gtataaactt tcaggcaatg aattatatgt acgggtaaaa gttatgggag
    27421 agcaaggtta tgttctgtgg acccagccaa tatataataa aaatatgttt gaaagaccat
    27481 aaaattagtg tacatacata taaggagact ttgataacag gagacttctt cgaaaatggg
    27541 gagggttctt ccataaaggg acggttctct ggaaataaga gtgtggaata tttccagaga
    27601 ctgttctatt aaaaataaaa taacgggtaa aaataaataa cggggagtaa aaatggataa
    27661 caaccgtaat gttcttctaa ttattaaaag tttgtatcct tcattacaca gggtagaaaa
    27721 aaagattgca ggatttattc tggagaatcc tgaaacaatt atatacatgc ctatagccca
    27781 gatggcaaag gatctggaag ttgctgaatc cagcatagtg agattttgta aaataatagg
    27841 ctttgatgga tttaaacagc tgaaaatcag cttggtcaaa agcttggaga acccagaaaa
    27901 aataatatat gaagatataa acaaaaatga cagtcaacat gagatcatta ataaagtatt
    27961 ttcaagtagt ataagaaccc ttcaggaaag cctccaaaca attgatgaga atcaatttac
    28021 aatggctgtg gaagcaatgc ttaaagcaaa aaggatagag ttttatgggg taggaacttc
    28081 atcaacactg gcacaggatg cgtattaccg ttttatgcga atagggctgc cggtatatgc
    28141 tgttgtagat ccgcatgttt gcagggtatc tgcatctaca atggataata cctgtctggc
    28201 aatagcaata tcctacaaag gcagaaccag agatactgtt gaaactcttg aaattgccaa
    28261 aagcaaaggt gcaagaacaa tgtgtattac atgctttcca caaagtccaa taactaaagt
    28321 atcagatatt cagatcatca tgcctacaag tgaagccaaa ataatgaggg aggcaatatc
    28381 atccagaatt gcccaaattg ctataattga cagtttatat acggctgttg ccctggaaaa
    28441 atttgataca gtagttgacc atattgaaga tttatcagat attctaagca gcgtccggct
    28501 ataagataag gtcgttatag atacgaattg gtatagataa aggctgttat agacaaggat
    28561 tggtatagat aataactgga ggctggatat gagaaatgag aagtaggggg acactataca
    28621 tggagaacag aacaatgaaa tactggcaga agagaatatt tatattatgc tgggcagcat
    28681 ataccattgc atacctggga agaatcaacc tgtctattgc cattccatta ttggaagaca
    28741 gcttgacttt agataaaacc aaccttggga ttattggcag tgcatttttt tgggtatatg
    28801 ctttgggcca attgataaat ggtagacttg gtgacaaatt tgcaagtagg gattttgttt
    28861 tttttgggtt gttaatttct gcaatattaa atcttttgtt tggctttttt actaacttaa
    28921 tagttatgac tgctttgtgg gcatttaatg gatattttca atctatgctt tggggaccat
    28981 tgatgaaaac tattaaccaa tggtttccac aaaagcaaaa gtccaagatg gctctttata
    29041 tgttttcatc ggttgtatcc gggtttattc taacctgggg cggtcttggt cagatatcat
    29101 cctatatggg atggaaagct gttttctgga ttccaggctt aatccttctt ctatattcat
    29161 ttatctggta tttatatgca agaaacagtc cggaggaagc agggttatgt cttaaggata
    29221 caaaaacata tactgaggat acagaattaa atgccgataa attggtgttt cacacaaggc
    29281 tttatttgat tgcacttgct ggtatacctc ttgggtttgt tagggaaggt attagtttat
    29341 gggcacctgc aatgctgctt gacaccttcc aattaaacct gaagtcaaca atgagtgcag
    29401 ctttatttat tccgcttttt aattttatgg gtgtcattgc tgcgagattg cttattatta
    29461 agttgcaaaa taatgaaaca aagctggtgg caatattttt tgcaggtggt ataattacct
    29521 gtaccttatt atatttttcc cggaattcaa gcattgtcgc ttatctggtt ttgctggctg
    29581 tgtgttcttt aagtctgtat ggcgcttctt caatcattac ttcagtaata ccgctaaaat
    29641 atcgtatgac ttcttcggta gccgggtttc ttgatttttc aatatatttg ggtgctggac
    29701 tgtcgggaat tataaccgga gctctttcca ataaatttgg atggagtatt gttgtgctgg
    29761 tttgggtgat agctggagca ataggaagta tatgcatgta catggcaggc aggagatgtt
    29821 ctaaccgtag cacagttgct aaagttgcta atgcggacct ttctcttatt gaatagataa
    29881 aaaaggaaga aataattata atccatatct cctacaattg ttgttatagt tgtttatgta
    29941 ttgtataata atcgtagaaa aagcaggaaa tgagtttttg tatcaaaata aactgttacg
    30001 ttaattatat tttaaataac agaattgtag gtaatgcaat ggctaagctt attaatgacg
    30061 ctattaacgc tattaatatt aaaaaaacat ccagcaggat atacagtaaa attaaaaaag
    30121 tcataagcaa ttgtgtcaac aatgttagca gtatagctaa agagtctaca ggtattttta
    30181 aaaagacaga taccagacag cagtttataa ttttatttgc tttcagtact atagttttag
    30241 ttgcaactgt atgtatcatt agctcagctt attcaaacac tatacttggt agtaacgaaa
    30301 aatatataaa taatgttgtt acgcagtata aaaatgagtt cacgcataac ctcagtgaaa
    30361 ttgatcaact tgtaaatggg cttgcctata atccagctgt aaagaattat ctaactataa
    30421 aagataaccc atatcaaagg catgtatacg cttctgaact aataacttat ttctattatc
    30481 ttaaatctgt taaagttgca ataaaggata tgacagtatt tcgtcttgat gcaggaaaag
    30541 tcacttatta tgattcaatt agacctgaat cgttgattgt tgatcttttc gaccaaaaac
    30601 cccattctta ttgttatggt gtagtagaat atggaggaga acatgggggg cgtcactata
    30661 gaaatttgct tagcgggtgt tcggtgtata ataagaatta tttaactgac gaagtagaaa
    30721 aaataggaac aattgtagta tcaatagatc cctatggact tagtataaag ctggaagaat
    30781 tagagggtgt tgaaagtgta aagtatatgg tttatgatga aaagaataat attgttatag
    30841 ggaacattga tactaataat gctgaaattg cagatatcat taacaaagct gaaaataatc
    30901 ataatgcaaa tcattttgaa acaaaacgtt acaaattgaa tattgttgaa gttgagggac
    30961 tccgaggcag aattcttaca attgtagata aaaatctttt actcggacct gtaaaacaag
    31021 ctgttttttt cagtggactt attctgttta tagcaatcat attgcttgta ttgatgtatt
    31081 acttagctgc tggaaatatt gttatgccaa ttaacgatat cattgtattt ttacaaacca
    31141 tgcgaaaaga ggatattagt aagcttgata aaagaattcc tattgtagga aataaagata
    31201 taagattatt ttcattagaa tttaataatg cgttggatga gattaatgca cttacatata
    31261 aactaattga taaaaactca cgcttatacg aaatggaaat tactaaaaaa caatcagaga
    31321 tagctttatt gagaagccaa ataaatcctc attttttata taataccttg gaagcaatac
    31381 gttcaattgc agtaatacgt gatgtacctg aaataaagga catatccaga tctctggcaa
    31441 gaataatgaa atacagcatc aaaggatatg aaaacgtgac attagccgaa gaactaggta
    31501 ttataaaaga ttatattcag attcaaaata caagatttga caataagttt tcaactgaat
    31561 ttattattga accggaagca cttgcctgta ctatacctaa aatgaccctg cagccaatag
    31621 ttgaaaatgc tatttctcat ggacttgaac taactccaga tgctgggcat ttattattac
    31681 atgcttatat agaaaatgaa aataggcttg ttgttcgaat tgttgacgat ggagtaggga
    31741 tagatgaagc tgatcttgct tccattactg aaaaattgga atctgaagat tatagtttct
    31801 atcttaatca ccgtgcaggc ataggcttgg taaatgtaaa ttccaggata aaacttacac
    31861 aaggtaaaaa ctatggagtt aaggtaaaaa gccaaaaaga caaagggaca gaagtaacaa
    31921 tatcattttt agcggaggta tccaacaatg tataaggtaa ttttaataga tgatgagcag
    31981 tggattatta agagccttaa aaagagtata ccttggaatc agtatggatt tgaggtttgt
    32041 gcaactgcag ctgatggaaa gattgccttg gaaaagatta atgagtttca ccctgatgtt
    32101 gtatttactg acattaaaat gccatatatg aatggattag atttaattgc caaggccaaa
    32161 cagagccatc ccgatttatt atcagtaatc attagcggat atgcagagtt tgaatttgct
    32221 aagactgcaa ttcagcacaa ggtagatgga tattgtatta agccaattga agaggaagag
    32281 attatcgatc atctcaagaa ttttagaaaa gagttggata atctaaagcg cagccggaat
    32341 aatttagacc tgctaaattg tattgaaaga aatgataaga agatgtttaa ggaaatatat
    32401 ccctgtgcag gcagtatttg tattatagtc tctattggtg ataaaattac ttatcttgaa
    32461 ggcaaaccga aagcattata ttgcaatgtt tccatgaata agtggcttta tttcttgccg
    32521 gaacctataa atatgcagca gattgaaggg aatctgagta aaaatatcaa aggtgttgga
    32581 atatttcaaa ttaatcttca aactgatgat ctggtaaaag gtattgacga agccaatact
    32641 agagcgcagc aatatttttt taatgaagat aataaagttt ttgcaggtga ttatgataaa
    32701 aataaaataa aaacctataa tcagctttta aatggaataa tagctcaagt taaaaataaa
    32761 gatattactg cattgagcag atcacttgag gagattaaga taattttgaa cagtataaat
    32821 attgtagaac ttaagaaatc atatagcatt ctaaacacat taaaatacct gaatataaca
    32881 ggagatgatt tagaagaaga tgaaacatgg atggatcagg atattatatt tttaaacaac
    32941 tggagtaatg tggaagagta ctttgaatat ttccgtcatg aaataaaaat attagttaac
    33001 attaagcatg aaagcattca gatatattca gggaataaac ttatttctga tgttatcaat
    33061 tatataaaag ataattatat gaaagatatt actttatata acttatctac agaatttaat
    33121 attaaccaat cttatttgtc acaactattt aaaaatgaga caggagttaa ttttgtaaat
    33181 tatttaatga actatagaat atcaaaagca tgcagcttgc ttttgcagac tgatatgtct
    33241 ataaataaca ttgcacaaga agtaggttat gatgattact tatattttag gaaagtattt
    33301 aaaaaagtta ataatatgac ccctacagaa tacagggaac aaaagatgag gttaataaat
    33361 aatgagtaac agaagataat acgaaaatat tacataaata ctataaataa tttctataca
    33421 accttctttc tttcttccat atactaataa gtaagtaaat taatactact atgccaattt
    33481 gggaagtgaa gtcttcaaat gcgctttcca ttggcaacat aacaaagtag tatttgagag
    33541 gaaaagaaat gatatgaaaa gtaaatttaa acttatatta ctattgatgc ttgtaataac
    33601 tttttttact ggttgcatca aggatatcac tgtatctcct gcggaacaaa caacatcctc
    33661 tgtaagcggc acaacacctc ctgcaaaaga atctgaaaat gaaataccaa tagagtaccg
    33721 aactgaagaa atttacgata gaacaaatga taatggcaaa ttggtatttt acttcatgca
    33781 aattgacgag attgataata ctacaaagtg gggagactct acgttaatat gttttccaaa
    33841 tggtgaaaca atgatagttg atgccggaaa aagctttgcg ggcgaacata ttttaaacag
    33901 tctagagcgt ttgggaattg acagaataga ctatgcaata gcatcccata tgcatgcaga
    33961 ccattataaa ggtttttata ccataatgaa gaatattgat ataggcgtca tgtacacacc
    34021 ggattttcca tgtttcccgg atgaacagta tactaataat tgggaaaatg cacttaaaag
    34081 ttatggagtt gaacatcgtc atcttgagat gggagacaca ttagatattg gagatgtcca
    34141 tattgaagta tttggtcctg aacttacaga agcaaatatg aatacagtat tatcacttgg
    34201 aaaagattct cccagaatag tgaacaacct taccagtatt gtaatgaaat taacttatgg
    34261 aaaaacctct gcgttgttta caggtgatat ttacatgata agagagatag aattgacaga
    34321 attatttagt gataaattgg aggcagattt attaaaggta ccccatcatg gacatgatac
    34381 ttcagggtca aaaatattaa tcgatacagt aaaacctaaa gtggcagtag caatgggaaa
    34441 tattctcatg gatccctata caacatatgc tcaatatgtt agagctgatg ttaaaatata
    34501 tattactgtt atagatggta tggtaaaggt tacaatggat ggaagtgata tggaagtaat
    34561 aactgaacat gacagacagc ccaatttccg caaaacatat gacgataaac gtataaaagg
    34621 attgactaat tattaattta acatgaatca ttgcactgtt atgcaactaa ataatactaa
    34681 ttacaaaaat aatacatata tgatacaaat aatttccata cgatttcact ttcacctgct
    34741 atatagtatt aattagataa aatcattaca aagtattcaa ttaatcgaac cttcaatcaa
    34801 ttaaagttat ttatcgaggt gagtaatgta tattaatatg actaaaaaag acagaactat
    34861 tgcagtaatt gatacattaa atgttgaggg aaatataaat gccaataatg ttaagaatat
    34921 gaagaca
//
LOCUS       DAOSDD010000014        34753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_628_length_34753_cov_5.320018, whole genome shotgun sequence.
ACCESSION   DAOSDD010000014 DAOSDD010000000
VERSION     DAOSDD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 34753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34753
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_34753_cov_5.320018"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1055)
                     /locus_tag="PK733_02335"
                     /pseudo
     CDS             complement(72..1055)
                     /locus_tag="PK733_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1064..2584)
                     /locus_tag="PK733_02340"
     CDS             complement(1064..2584)
                     /locus_tag="PK733_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ09414.1"
                     /translation="MAWSGAFDFFDFASDDIYNTTYDVVVIGGGVIGCHIAKQLSRYK
                     INICLVEKENDVACRTSGANSGIVHAGYDAFPGSLKAKLNIRGNEIIEHIAEELDVPF
                     KRTGSLVLAFDEYDMTMLKELYERGIKNGVTGMEILDEKQVREKEPAVSAKVVGALFA
                     PTAGIICPYELTISAAEVAVENGTRLLLSHEVISIEKVYRDKERTLYQITIKHKEKGP
                     KKIFAKYIINAAGVYANKISSMAGDTSFTITPRKGEYLLLDKNQGRIINSVVFQTPSK
                     MGKGILITPTVDGNLLLGPTASDIEDKEDLSTDNEGLTKVRLGALKSIPGIGMENIIT
                     SFAGLRAVPSTGDFIIEESNYAPGFVNVAGIESPGLTAAPAIAEYVEDLMREAGLELL
                     PKENYKPNRTPFKKFRNMSDSEINEAIKENSLFGKIVCRCEKVTAAEVVEAIRRPAGA
                     RTLDGVKRRTRAGMGRCQGGFCTPRVMEILSQELGIPMEEITKSGGKSWMLAGRSK"
     gene            complement(2983..3564)
                     /locus_tag="PK733_02345"
     CDS             complement(2983..3564)
                     /locus_tag="PK733_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HOJ09415.1"
                     /translation="MNKVNLRNNVINNIEKNPIIAAARKDEDIEYAVNSGVAAVFLLR
                     TDIFSAESHVGKIKASGKNVFIHFEFFEGLGKDNKAIDYIANVIKPDGIITTRTSHVK
                     YAEEKGIFTIQRFFIVDSQSYETAIKSVQTVKPSMIEIMPAVMPSIVQRFCRDVNLPV
                     IAGGLINSKEDIIDILKAGALGVSTGKKELWVL"
     gene            4010..4270
                     /gene="spoIIID"
                     /locus_tag="PK733_02350"
     CDS             4010..4270
                     /gene="spoIIID"
                     /locus_tag="PK733_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOJ09416.1"
                     /translation="MKEYIEERAIELAYYIIEKKTTVRAAAKKFGISKSTVHKDVTER
                     LERISPELADQVRTVLDENKAERHIRGGQATKAKYNSEIKAG"
     gene            4653..5681
                     /locus_tag="PK733_02355"
     CDS             4653..5681
                     /locus_tag="PK733_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ09417.1"
                     /translation="MAFGQDIGIDLGTATILVYIKGKGIVLREPSVVAIDKNTNKMLA
                     VGEEARRMLGRTPGNIVAIRPLRDGVISDYDITEKMLKYFLNKVCGGRVIKLFKPRII
                     VCVPSGVTEVERRAVTDAAIQAGARKIYLIEEPIAAAIGAGIDISKACGSMIVDVGGG
                     TTDIAVISLGGIVVSTSIKIAGDKFDESIVRYMRKKHNIMIGERTAEELKINIGTVYP
                     RVQEVTMDVRGRNLISGLPKTITVTSTEIMEALEEPISTVVEAVHSVLEKTPPELAAD
                     IGDRGIVMTGGGSLIYGLDKLIQKETGINVLIAEDAVSSVALGTGKALESIEEIEYSY
                     ASESKIKR"
     gene            complement(5936..6982)
                     /locus_tag="PK733_02360"
     CDS             complement(5936..6982)
                     /locus_tag="PK733_02360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ09418.1"
                     /translation="MKKFYSEKKGSEKSIFQVFNKKGFYIILALSVVIVAGAAVLITR
                     QNLDFLNLKSEKENFIPDDFENYLTDFENDLNSNEVDRHEDMQTASNDLQTEKTTGIS
                     EVEKDVKQLGNNEEVSGSGNSRESSITSENNTTADNTIAAKTDDSEALKIPGIKPKDK
                     QEDKQEDKQEEKQDKKKDREQDNKKPGEEKDAAPENKDDKKSNDSKTAEGIEKDFIMP
                     VYGTIINDFSMDKLVYSKTLEDWRTHSGIDISAVRGTAVKAVADGVVSEVREDPKLGY
                     TVIIDHENGIKTVYANLASADMVVTNQIVSQGESIGSIGDTAAYEIALEPHLHFEVIK
                     DGVLEDPKLYLPNY"
     gene            complement(7071..8174)
                     /gene="spoIID"
                     /locus_tag="PK733_02365"
     CDS             complement(7071..8174)
                     /gene="spoIID"
                     /locus_tag="PK733_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOJ09419.1"
                     /translation="MKKVIYFIVSMIIIVVVLPMLIVKSCSFNVIGNGQGQIQNRKQD
                     KDEENDDNKNDINNQISDEENKKWQDNIKIKVYIESEDKVKEIGFEEYITGVVAAEMP
                     AEFEVEALKAQAVAARTYAYGRLNGLYKNSNDIHSNADVCTDFAHCQAWISKEDALKK
                     WPQDKANQLWDKVRNAVLDTAGIIIIYKGNIANPVFHSTSGGKTENAEDVWDVGPVDY
                     LKSVISEGEEGSPSFKTISNISVESFCKTLKNCYPNIKLNDKNLFGDIEILEYTGGER
                     VKTIKIGNITLKGTEFRKVFSLKSANFELEKGENNMVKIMTYGYGHGVGMSQWGANSM
                     AKKGSAWEEIIKHYYTGVDLDIIDDNRYIFSTK"
     gene            complement(8289..9548)
                     /gene="murA"
                     /locus_tag="PK733_02370"
     CDS             complement(8289..9548)
                     /gene="murA"
                     /locus_tag="PK733_02370"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ09420.1"
                     /translation="MAKFIINESSELKGIVRISGAKNSALPIIAASLLADGESIIEEV
                     PYLNDVVIMCNLMEFLGSKTVLSEKEKTLVISSPNINSTAAPYELVHKMRASFLIAGP
                     LLARTGYAKVPLPGGCAIGTRPVDLHLKGFAALGAQINQGHGYVEVKAEKKLKGAKIY
                     LDFPSVGATENILMAAVLAEGQTVIENAATEPEIVDLATYLISMGADIKGAGTDTIKI
                     NGMDSIKGTRHSIIPDRIEAGTFMAASAITGGDVVLENVVPDHLKPVSAKLTEAGVEI
                     SEELTSIRVVSDGKLKAVDIKTHPHPGFPTDMQAQMTSLMSRASGTSMVVETIFENRF
                     MHISELKRMGANIRIEGRSAVIEGKSKLMGTQVKATDLRAGAALIIAGISAEGVTEVE
                     DIEHIDRGYVKIDEKLKNLGVDIYRQD"
     gene            complement(9565..10413)
                     /locus_tag="PK733_02375"
     CDS             complement(9565..10413)
                     /locus_tag="PK733_02375"
                     /inference="COORDINATES: protein motif:HMM:NF020264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HOJ09421.1"
                     /translation="MMKKGFLNSAILAGSIFTLIIVLVFSFSYYFNKRKLYKVPEEAL
                     IESFESSGAVYVSSEIYFTAALDKKYEKDDYLNKLLNDIAQSLNLDLEGNSSSEDNSV
                     SMDVYESDLVRKLVISGEKSSGESISISVELLKMTDSPKLPEMSGLPKSLEQPGRKIT
                     VGVTGSFPYSGLPLISKSVEAAFKMNKVKPVTNSCIIGNYQGKIDYINMNEVCKNIFS
                     ILGAKKVGNVAQNNLVSVSAYSPAIGNSIKVDRNKSNIDIAVRYNGYEQKTYIWIATP
                     AISMTY"
     gene            complement(10764..10973)
                     /locus_tag="PK733_02380"
     CDS             complement(10764..10973)
                     /locus_tag="PK733_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09422.1"
                     /translation="MGDVLSSYTYRQGIENAIGGKVGAVMWLSAAFQIIRAAMLVYLF
                     ILFVKLVTRGIKALDLYIDEKTNIK"
     gene            complement(11327..12604)
                     /locus_tag="PK733_02385"
     CDS             complement(11327..12604)
                     /locus_tag="PK733_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ09423.1"
                     /translation="MMDIIMRSSEKSGINDIELKKALECSIEAKKAGLKKVLLLPPDI
                     TRAHSGAGKITAMYYEMLKDTCQVDIMPALGTHEPMSEEERREMFGDGIPMDRFIPHN
                     WRTDVVKVGTVPGEYIEEISEGLLNEPMDIEVNKRLLDKSYDLIVSMGQVVPHEVIGM
                     ANYSKNIFVGCGGSSTINNSHMLGAVYGMEKLMGKDHSPVRKVFDYAEENFIKDIPLM
                     YVLTVTTAVKGNVSIHGVFIGRNRKVFEESIKLSQEKNLTFIDKPFNKVVVYLDEKEF
                     KSTWLGNKAVYRTRMAIADDGELIILAPGVRKFGEDDGNDALIRKYGYVGRKKVLELT
                     KQNADLQNNLSVAAHLIHGSSDDRFSITYAVEKLTKEEVEGANFKYMPLSEAYKKYNP
                     AKLKDGYNTLEDGEEIFYISNPALGLWADKSKF"
     gene            complement(12734..14185)
                     /gene="gnd"
                     /locus_tag="PK733_02390"
     CDS             complement(12734..14185)
                     /gene="gnd"
                     /locus_tag="PK733_02390"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOJ09424.1"
                     /translation="MSKSDIGLIGLAVMGENLVMNMESKGFTVAVYNRTVEKVKNFVE
                     GRAKGKNIIGTYSLEELVGSLKKPRKIMLMVKAGKPVDDFIDMLIPLLEKGDIIIDGG
                     NSHFPDTIRRTEYVESKGLLYIGTGVSGGEEGALLGPSLMPGGSPDAWLHAKPILQAI
                     AAKVEDGSPCCDWVGENGAGHFVKMVHNGIEYGDMQLICEAYHLMKDLLGMSADEMHE
                     VFKEWNEGELNSYLVEITRDILAYKDDDGQPIVDKILDTAGQKGTGKWTAIAALDEGI
                     PLTLIGEAVFARCLSALKEERVEASKVLSGPAVKFEGDKKAFIEDIKNALYASKIVSY
                     AQGFTLMRAAAKTYNWHLNYGGIALMWRGGCIIRSIFLGKIKEAFDKNYELTNLLLDP
                     FFKEKIEAAQAGWRRVTATALSNGIPVPAFTTALCYFDGYRSERLPANLLQAQRDYFG
                     AHTYERVDKPRGEFYHTNWTGRGGSTSASTYIA"
     gene            complement(14326..15225)
                     /locus_tag="PK733_02395"
     CDS             complement(14326..15225)
                     /locus_tag="PK733_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOJ09425.1"
                     /translation="MINPAQQLIDMKKENDFLICIDSDGCAFDTMEVKHKECFIPNII
                     NEWDLQAISKYVREAAEFVNLYSKWRGINRFPAIINVMDLLADREEVKKRGFKVPNLG
                     PLRKWIEGETKLGNPALETEVRRTGDPLLAKTLKWSKAVNDTVAKIVRGVPPFPFIRE
                     SLEKMLEYADIIVVSATPGEALIREWEEHDISKYVKVLAGQEMGTKAECIHFAIGAEY
                     ENEKVLMIGDAPGDLKAARTNNALFYPINPGNEDDSWERFYSEALDKFINGQYAGAYE
                     EKLIAEFDKYLPSTPPWKKSSVK"
     gene            15517..16374
                     /locus_tag="PK733_02400"
     CDS             15517..16374
                     /locus_tag="PK733_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09426.1"
                     /translation="MQTLIHDFTSRQYMITPDFEYFHYIDRSANEIEYHNHDFYEIFY
                     FMSGNVTYMIEGKSYRLKPGDILLIHNNELHKPLVESGGVYERIVVWVNPDFLKKQSL
                     YNTNLSLCFESSFPEKNNLIRPEPEMLDVLKDILAKFENACNSVSYGSNILKDIYLLE
                     FIVYINKSFLQSFGDKLDEDIEYDEKVNNIIKYVNKNLNDDLSLDSLAQKFYLSKYHL
                     LREFKKYTGYTIHQYIHQKRLIMAKSLLKEGMSVTEVCNRCGFGDYSNFIRAFKNAFG
                     ESPGKYFKD"
     gene            complement(16371..16619)
                     /locus_tag="PK733_02405"
     CDS             complement(16371..16619)
                     /locus_tag="PK733_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09427.1"
                     /translation="MYHKNIDDNDINQVNQVNHDINLTLEEISSGINEGETLILDVSD
                     NHFSQMDKIFDTLIMRGYDVRKSFFKGRNQIVVSRKRS"
     gene            17121..17999
                     /locus_tag="PK733_02410"
     CDS             17121..17999
                     /locus_tag="PK733_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09428.1"
                     /translation="MVGIIGNIVNTGNIMNTRNVGNFRNTGNVGNTRDDGNMSYRSNN
                     NNKSSIVLISINNLTKSYAKNLAVDNISLDIFKDEIFGFAGPNGAGKSTLISLLTTLI
                     KPDKGDIIINGYSVFTEAFQIRKMIGFVPQDIALYPTLTGYDNLKFWGGLYGLRGREL
                     SARIDEALAVVAMKDRAHDKVEEYSGGMKRRINIAAALLHHPDILLMDEPTVGVDIIS
                     RHYIIDAIKNLKREGRTIIYTSHDIEEMEMVCDRVAIMNYGRILKCDTVEKLKRDGKN
                     HTLKDTVLNVVLSEEH"
     gene            18111..19847
                     /locus_tag="PK733_02415"
     CDS             18111..19847
                     /locus_tag="PK733_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09429.1"
                     /translation="MKTALKKNTNMPNMSKKNLYIIIAAALVIVAAAVLVTILLLNSG
                     KNVKDLYFQTEIKNFIKLMSDAEKRQNDKALEMKPFLEKPSRTRLELSAKLPHGSSSN
                     VSVSSGGNSGYGYGDLPGQAIDIINSSKLVLNSRYNLKEGLNASTLSFLLEGQSFLDI
                     NTFSENDTIGLQVPVIYDKYFVLDRNNLPSALKKFGVDMPVKKIFNVSEIKNAATFSL
                     EASKNIFTDYLKFLEENIQEGNISMSKNVKMKEMSYQTGTNASTDINASNNNSSTSGT
                     ISQEPGKSASKANKESRYNIFNVKLDEEEFKTISKKTVEVFLSDSRFVNMTMGNLTSV
                     FSMLEEAGYLNIFPSLKPAAAEILNYRDTEKLKTELINIIDTTAFPEGFNMTLVVDAV
                     GNFTDRKINLSSKADDEPARKYSIHAGRFFTKAIIEQESSVSGNAGSTDDKAVIEFEH
                     VKGSNPGDTYMHLNCVNNIWPDFDLTVNSSTESSKDDKRKAINTNCRVNMRLTASQLG
                     FDNANILLDLKREDRYDIDLNIPELNNESAINLTTADENQVNALVMELQFSAAKFLLS
                     NQSILNAFSSDN"
     gene            19899..21146
                     /locus_tag="PK733_02420"
     CDS             19899..21146
                     /locus_tag="PK733_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ09430.1"
                     /translation="MRILVIIKNDMKLFFSDWKAVMLFFGLPFAFVSIFILSLSPLLN
                     QNKFIESFNIALIDKDNTLESRMLINQFINTGKAVEDPNDPDYSGTSIKFIKTGEAAA
                     MDMLKNGEAAGILIIPEGFVYSMSIGENKPLKVITDPGKPLIADLIKGYMDSYANIIS
                     SAQNGIMTAYVFYEKAASGEEFYERKYNDVVTKFSLKALSKSDIFSARKVSYVPNVTK
                     YEYFTAALLVVFIMFSGIMGIKFTAGEKLHGISNRLNISPLKDIEFVFARFVTVFILS
                     MLQFLALIIPGGLVFKIYLNASPFYMLIMFIMTVFTISSCAIFIAVVSPTPATADLAG
                     SLGTLLMAAAGGSIYPLTALPDVVKNLSNFTINRWAVHGFLEIFSGQISSSFYTDIAV
                     LALMGITYLGMSVCLIRLSGNSR"
     gene            21195..22475
                     /locus_tag="PK733_02425"
     CDS             21195..22475
                     /locus_tag="PK733_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ09431.1"
                     /translation="MYKIFIVLKYKLKILLSDKSFLIAMTFIPLFLTLITGYALKYEE
                     ENKIPVAICDLDNTDYSKSIIERISAKEGFKFILTDEKTALKLVKDYKAEAAYIIKKG
                     FKENLLSGNTGGLIEQVYSPSTISSDIISEVIGSEVARIMLNTTAADWVVNEYNKMGI
                     LKDPKIPKDPKDSKDSKTQNNALWQEAWEYTDSLWDQGPVMGIEYGEIDSTSNGTAGK
                     NKNLPGNITSSAFGMLVAFVMFLIMFNSSWLIDEKENGTLRRIISGSGALNALFAGNI
                     LSLLFVGTLQIIFFSLICTIVFGVNIFSQGINVLIIILYLLSVIGISLFISSLLKTRM
                     QLQAGAPLFSIITAFLGGCFWSFKDMGGLIKIISLLTPQGLALDLFTKYSASVSGISF
                     MTAAGILMTSWQAIALLITAIALTSFSYFKIKLL"
     gene            complement(22467..24458)
                     /locus_tag="PK733_02430"
     CDS             complement(22467..24458)
                     /locus_tag="PK733_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09432.1"
                     /translation="MSKKHFIMNKRRVNSNKKYFKLLISVLVIAGLVITTLFATGVIP
                     PGGFHTTSSDSGRNSKAKAGDGIDTEEAFDQQEETVDGSMTDDSTSDNNIIDDSTIDG
                     SITENGYKIAIENNSPEYDVEIYLYFDNNKKPFIEADYYLNGISRSRLYKEDDIPGLK
                     DIMDKETSAGSGNLGGSVTSGVSGTSGDNTGTSDTEKSNPFSISGAILNTKYAKVYFC
                     IKKDLKPEESAYNLYALSLKDGSIRPLYQWKGNSLNSPVFSPDKEYIVISYLKDINGN
                     NSFVHIFNSSDDEGLVVDNRTKDGQLIGRNRSDEKGKNYSYYIVRWKSEEDLKLREYS
                     YIPDKDMNPVKDEQSYEITYNVLKNQLTYPEEKYSQSTASKPAETSQTKPAENSQNGT
                     VESVSRDGTGGSSSNGSGTSSSTGNGTNGTNGTNGTDRTGGTGTGTDNSTNGSTSTGT
                     NGSTNTGENGSTGTGTGSSTGSGTGSTSGNVSDTGTGNGNGNGSDSGSGTGTGTGNGT
                     GTSGSTDSDVNNSNGTSNGNNTGNDSQSQINSEAVKVIKSFYAYVSEGKYEKAYDLFD
                     DNFQSESQMFMGFKISKAEITLEVFSAFAEATEIFKNVKVEKVLKEELQGSNSKIHYT
                     QSMALDPNKEPLVYSLVATLKKAGGVWKIITVEDGKLSK"
     gene            complement(24530..25645)
                     /locus_tag="PK733_02435"
     CDS             complement(24530..25645)
                     /locus_tag="PK733_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ09433.1"
                     /translation="MKDPRMEKLAKMLINYSVDIKPGENILIETTGEGDELARELIKA
                     TYEAGGVPFITLKNTETNRVLLENCTIEQIDRTASYEIARMKDMKAYIGIRAPENVSE
                     MGAVPADKMKIYMEHYSKPLHSELRVKHTRWCVLRYPNNAMAQLAGMATEYFEDFYFD
                     VCTLDYPKMSKAMDSLVKFMEKADKVRITGKGTDLSFSIKGLPAIKCDGKMNIPDGEV
                     FTAPVRDSVNGEITYNTPALYQGFTFENIHFQFKDGKIIKAEANDSERLNKVLDTDEG
                     ARYIGEFSIGLNPYIMKPMKDTLFDEKISGSIHFTPGNAYDECNNNNKSAIHWDLVFI
                     QRPEYGGGEIWFDDILIRKDGLFVPEELQCLNPENLK"
     gene            complement(25677..27461)
                     /gene="pepF"
                     /locus_tag="PK733_02440"
     CDS             complement(25677..27461)
                     /gene="pepF"
                     /locus_tag="PK733_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOJ09434.1"
                     /translation="MLVRSEIDNKYKWKLEDIYSSDEVWESDFDKVKKMIPDIAKLNG
                     TLGESSGKLLECLKLRDNILSISEKLYAYARMRRDEDNSNSKYQALNDRAFALLTELS
                     GAISYIEPEITGLGKDILKGFIDENNELKIYSHFIRDILREKDHILTQKEEEILALVS
                     EIAQAPADIFTMLNNADIRFPFIKDENEKEVELTKGNYIKCLESTDRRVRKDAYEGLY
                     GVYKSFRNTFASSLSNNIRKDRFFASARKYNSSLEASLSEDNVPSQVYDNLIKTVNDN
                     LHLLHGYMGLRKKTLGLDKLCMYDVYVPLVKEPMSNMPYEEGYALVEKGLEPLGQEYS
                     SHIKEAFNSGWIDVYENEGKTSGAYSWGSFLTHPYVLLNYQGTIKDAFTIAHEMGHAM
                     HTFYTNKTQPYIYSEYKIFVAEVASIMNEFLLMDYMISHSKNKEEKTYLINSYLEDFR
                     TTLFRQTMFAEFEKVAHNKVAEGEALTAQSLCDIYRSLNEKYFGSNVEIDDEIAIEWA
                     RIPHFYTSFYVYKYATGFSAAASLSKQILEEGKSAVDRYIKFLSSGGSDYPLELLKKA
                     GVDLTVPAPIEDAMKKFESLLCEFEKML"
     gene            27939..28015
                     /locus_tag="PK733_02445"
     tRNA            27939..28015
                     /locus_tag="PK733_02445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27973..27975,aa:Arg,seq:cct)
     gene            28341..30980
                     /locus_tag="PK733_02450"
     CDS             28341..30980
                     /locus_tag="PK733_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ09435.1"
                     /translation="MAINLIDLVNLIFNSRDFIIALFIFLLYTYLFFIDRKKYIIVWL
                     TGWGAILLLSILCALKSQDIGLIITAILTDPLLLNLLLLFGVFILLNGTYLFFGYKLP
                     IIWILVTSLTSAYLVIVRFSYPGSYSVLNLPYRIIADLLTLWIGILFLRNSEIEKVSK
                     YITGSSYILLGIIKLCLPYIGQYPIYKTLSSIFYNLLYIFIALGTFLIFFEKVKGDSN
                     ITERKFLGAFETSAIGIAIVSKSGKFIEVNKALCNITGYAEEELMSKTFQDITYHEDI
                     NIGLEYLSQVMSGEICSFQIEKRYIHKDRHTIWSLLNVSLIKNNSGQPLYLVSQILDI
                     SDRKSTEELLKKSEERYKLLLESIPDIVAVLDHNSRYTMVNNAVLRLVNKKSREDLLG
                     HKITDILPGFEETPYYKAYKRVMETQNPVTIIDKYRYPQQEGRSTWYEVHSFPVPEGV
                     MFISSDITGRMRAIEMLRDSQDFIYAVLNTVDALVLAADPYGRIITFNHACEKISGYK
                     LNEVKNKYIWDILITPEEIEKVKSLFFNIKPGETQVKLENFWIAKDGNRRLISWSNTS
                     LLDKNGSIKHVIATGIDITEQKKAERLEAEIKEKTTLLIQTLELDRLRTEFFANLSHE
                     FRTPLNIILGSTQLLFMYLENESAGINANKFSSKLKYIKQNCYRLLRLVNNLIDATKI
                     DAGYLELNPLNCNIVNIVEEIALSVAEYIKSKNLNLTFNTNIEKKIIACDLDAIERIL
                     LNLLANAVKFTTSGGSIYMYLFVEENYVSITVQDTGVGIPEDKLGQIFERFRQVDKTL
                     TRSHEGSGIGLSLVKSLVEMHGGTISVESEYLKGAKFTIQLPARLIESNDGTPYGYNN
                     HNNNNIEGSFIERMNIEFSDIYF"
     gene            complement(31070..31318)
                     /locus_tag="PK733_02455"
     CDS             complement(31070..31318)
                     /locus_tag="PK733_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOJ09436.1"
                     /translation="MLIINLLGFVAAAADKHRARKKMWRIPEKYFFLISIFGGCPGVY
                     LGFIIFRHKTRHLKFMLGIPLIFLVQAVLIFYVLKTIY"
     gene            complement(31520..32068)
                     /locus_tag="PK733_02460"
     CDS             complement(31520..32068)
                     /locus_tag="PK733_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HOJ09437.1"
                     /translation="MKKFVCTVCGYVHIGNEPPEKCPQCGAPASKFVEKKGDNMQWAD
                     EHRIGVAKDVDPEVFEGLRANFMGECTEIGMYLAMSRQADREGYPEVAEAYKRIAFEE
                     AEHAAKFAELLGEVVYADTKKNLELRVEAEFGACKGKKELATRAKQLNYDAIHDTVHE
                     MCKDEARHGQAFKGLLDRYFNK"
     gene            32250..32408
                     /locus_tag="PK733_02465"
     CDS             32250..32408
                     /locus_tag="PK733_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09438.1"
                     /translation="MNYRELLELTKKSPNPFSINGRKTRLYDYEVRNKIPENSKKKNE
                     VYKRDRRS"
     gene            33063..33557
                     /gene="tadA"
                     /locus_tag="PK733_02470"
     CDS             33063..33557
                     /gene="tadA"
                     /locus_tag="PK733_02470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOJ09439.1"
                     /translation="MFNLVSGITHDNYWYMNEAYKEALKAYKKDEVPIGAVIVKDGKI
                     IGRGHNERELKNDATLHAEITAIKKACRKLGSWRLNDCDMYVTLEPCAMCAGALIQAR
                     IRTVYIGTPDPKAGAVGSVVDILSIEKFNHKVDVVYGIMEDECSAILKSFFKELRTKK
                     KYDP"
     gene            34002..34089
                     /locus_tag="PK733_02475"
     tRNA            34002..34089
                     /locus_tag="PK733_02475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34036..34038,aa:Ser,seq:cga)
     gene            complement(34107..>34753)
                     /locus_tag="PK733_02480"
     CDS             complement(34107..>34753)
                     /locus_tag="PK733_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ09440.1"
                     /translation="AEQMQYYIEFIVGDIIEIPILFGGSYGMRRSECLSVRESQFDFK
                     HKFFRANHTVTTATIDNKKIYIPSDKLKTDLSNRTYPLIPYVEERIKAKIAENKELKE
                     LCGNSYSKEWLGYICVHPLGEIIDPDYITNRHRDLLKKAGLPSIRFHDLRHSCVGLMM
                     ANEVPMERIRDWVGHSDIRTTVNTYGHLEYQSKKKTAKIIQKSLPLKKAGAANL"
BASE COUNT        10586 a         6786 c         5743 g        11638 t
ORIGIN      
        1 cataaattcc gccggaggca tataatatcc tcatttatga ggatattata tcatagagaa
       61 ctgtccccat gtttcacaaa ttgtgctgct cctctgcccg cgagcctgct ttcttctgtt
      121 acatgatcta ccaggtcatg aacatggact acatttccac atgcaaagat tcccggtatg
      181 gaagtctcca tggattcatt tacaataggt ccccgggtta acgggtcaat gcggactccc
      241 gcttcacggg acaattcatt ttccggaatc aatcccacag acagaagcaa ggtatcacat
      301 ccaatgtatt cccgggtttc aggcaagggt tttctgtctt catcaaccct ggctactgta
      361 actccttcca gtctttctct cccatgtatt gctaccactg tatggcaaac tctcagtgga
      421 atattatagt cctcaagaca ttgcactatg tttcttttta agcctccggg gtatggcatc
      481 aactcacata ccattttaac ccgggctcct tcaagtgtca ttcgtctcgc cataataagt
      541 ccaatatctc cggagccgag aatcacaaca tctttccccg gaagatagcc ttccatattt
      601 attagccgct gggcagttcc tgcagtgtat ataccggcgg gccttgtacc agggatgttc
      661 agagcacccc tggttctttc tctgcagccc atggccaata ttactgcttt ggcctttaat
      721 tttaataaac cctcttttga attaactgcc gttacaattc tttctccgga tatatccatg
      781 tcgctgctga tgtctataac catagtgtcc agcatatacc gaattccaag ggttactatc
      841 tgttgtataa acctgtgggc atattcagga cctgtaagct cctccttgaa aatttcaagt
      901 ccaaaccctg tatgaataca ttgttgcagg attccaccca actcttgatc ccgttcaagt
      961 atcaggatgt cttctacgcc atatttcctg gcttctatag ctgctgcaag tcccgcaggt
     1021 cccccgccta ttacaaccaa atcaacattt tccatatttc ttctcacttg ctccttcctg
     1081 ccagcatcca tgatttccca ccggattttg ttatctcttc cataggtatt cccagttcct
     1141 gtgacagaat ttccataact cttggtgtac aaaacccgcc ctgacatctt cccattcccg
     1201 ctctggtcct tctcttaact ccatccagcg tccttgctcc tgcaggtctt cttatagcct
     1261 ctacaacctc tgcagcagta actttctcgc acctgcagac aatcttacca aatagggagt
     1321 tttcttttat tgcttcatta atttcactat cactcatatt tctgaacttt ttaaaaggag
     1381 ttctattagg cttgtaattc tcttttggca gcagttccag gcccgcttcc ctcatgagat
     1441 cttctacata ctctgcaatg gccggtgcag cagttaatcc tggagactca attccagcaa
     1501 catttacaaa acccggggca taatttgatt cttcaattat aaaatctcct gttgaaggta
     1561 cagcccgtaa ccctgcaaaa gaagttatga tattctccat gcctatacca ggtatagatt
     1621 taagcgcacc caacctcact ttagttaaac cctcattatc tgtagatagg tcttccttat
     1681 cttcaatatc tgatgctgta gggccgagaa gcagattgcc atccactgtt ggagtaataa
     1741 gtattccttt gcccattttg gaaggtgtct gaaacactac cgagtttata atcctgcctt
     1801 ggtttttatc caggaggagg tattctccct tccttggggt tatggtaaaa gaagtatcgc
     1861 ctgccatgga agatatttta ttcgcataaa ctccagctgc gtttataatg tatttggcaa
     1921 aaattttctt tggccctttt tctttatgtt ttattgttat ttgatataaa gttcgttctt
     1981 tatctctgta tactttttct atactgatta cttcatgtga cagcaacaac cgggtgccat
     2041 tttctacagc aacctcagca gcactgatgg tgagctcata aggacaaata atgccggcag
     2101 ttggtgcaaa taatgcacct actactttag cggaaacagc cggttctttt tctcttacct
     2161 gtttttcatc caatatttcc atgccggtaa ccccattctt tatacctctt tcgtacaatt
     2221 cttttagcat agtcatatca tattcatcaa aggcaagtac aagtgaacct gtgcgcttaa
     2281 atggaacatc cagttcctct gcaatatgtt caataatttc gttaccgcga atattcagct
     2341 ttgctttcaa actacccgga aaagcatcat accctgcatg cactatccca ctgttggcac
     2401 cgctagttct gcaggccaca tcattttcct tctctaccag acaaatgttg attttataac
     2461 gtgaaagttg ttttgcaata tgacagccta taacaccacc acctataacg acaacatcat
     2521 aagtagtgtt ataaatatca tcagaagcaa aatcaaaaaa atcaaaagct ccactccaag
     2581 ccatatattt acgcccccct cttacacctc taagtgcacc tgctttaccg ccaagtgcca
     2641 cccaccatac ttctaaatgc agtatgccat actacccaag tgctacctgt cagatttttt
     2701 actgtaatga atttaagccc ataaaaactt tttcatataa agttttgttc ctgcaagggc
     2761 gaattcattt cacacacagc aggaatggag cttaaaataa aaaagccaca ccaagttaga
     2821 ccctatttaa ggtctaagct taatgtgact ccaattctcc acacattata cattgctatt
     2881 tgctattata ttttacaaaa catcagtaaa ttataaatat actactacta taacactatc
     2941 atatcacatg aatatcctag gtgcaagact ataatatttg tttcaaagca cccaaagctc
     3001 tttttttccg gttgatacac ccagtgctcc tgcctttaaa atatcaatta tatcttcctt
     3061 actatttatt aatccccctg caataaccgg aaggtttaca tccctgcaaa atctctgtac
     3121 tatacttggc ataaccgcag gcataatttc aatcatgctg ggttttacag tttgtaccga
     3181 cttaattgcc gtttcatatg actggctgtc tacaataaaa aaacgttgaa tggtgaagat
     3241 acccttctct tcagcatact ttacatggct ggtccttgtt gtaataatac catcaggttt
     3301 tattacatta gcaatatagt caatagcttt attatccttt cccaagcctt caaagaactc
     3361 aaagtgtata aaaacatttt ttccactggc tttgattttt ccaacatgac tttcagcact
     3421 aaaaatatca gttctaagaa ggaataccgc agctacccct gagttaacag catattctat
     3481 atcctcatct ttacgggctg ctgcaataat ggggttcttt tcaatattat ttattacgtt
     3541 gtttctaaga ttcactttat tcacattaat cttctcctga tcatgtacaa aaaattgtct
     3601 ttctgaatac taaatgtagt aaagcttata tatagtacaa cacaaataaa aatgtttttc
     3661 aatagtatta atgtatcgca ctccaatcac tgtatttcta ttttataata tccattcctc
     3721 tttactttac gaagcagtac accatcttat aaatatatta tacaacgagc atatattctt
     3781 ttttagaaga aaacatatat acatcctcag gaaattgaca ttttacgtac attctcttta
     3841 attgccactc ataagctata attggcaggc atatatatac tgtagtaata caaaatctgt
     3901 taatacatta ctatactaaa ctgcatattt ttaataagcc cggataaaga cgcatttatc
     3961 tgcgtttagg aacatagcat ctcaatgttg ttcaaagggg ggttgggcat tgaaagaata
     4021 tatagaagag agagcaatag aactggcgta ctatataata gagaaaaaaa ctactgttag
     4081 agctgcagcg aaaaaatttg ggattagcaa aagcactgtt cataaggatg tgacagaaag
     4141 acttgaacgt ataagtcctg aactggcaga ccaggtaaga acagtgcttg atgaaaataa
     4201 agctgaaagg cacatccgag gaggtcaggc aacaaaagct aaatataatt cagaaataaa
     4261 agccggttag ccggctttta ctataacgaa acagcttaaa tttgtaaaac atttcaccag
     4321 aaacattttt aatgtatata gaagcctatt attgatgata atttataatg agaataattt
     4381 gcaatatagt aaaagtaaat aataaaatat tttaaatatt tgcgttaaga gtagtaattt
     4441 tttttcgtgc ataagcactg ataaagttat tgcaatattg taattatatg ataagattat
     4501 ttatatataa tatcacaaaa agacgttatt tccttaaaaa gtacgctata acgcttccac
     4561 ttcattgttt tgcatacaat aataggttgt tttgaaactt tttgaggaaa atagtatata
     4621 tttaatactc gggaataaag gggatgttgt tattggcttt cggacaggat attggaatag
     4681 accttggtac tgcgactatt ctcgtctata taaaaggtaa gggaatcgtt ttgagggaac
     4741 catctgttgt ggcgatagat aaaaatacca ataaaatgct tgcagtaggt gaagaagctc
     4801 gaagaatgct tggccgcaca cccggaaaca tagttgctat aaggccttta agagacggtg
     4861 taatttctga ttacgatatt actgaaaaga tgctcaaata ttttttaaat aaagtttgtg
     4921 gtggacgagt tataaagtta ttcaagccaa gaataatcgt atgtgtcccc agtggagtaa
     4981 cagaagttga gagaagagca gtaacagatg ctgcaattca agccggtgca agaaaaattt
     5041 atctcataga agaacctata gcagctgcaa tcggtgcagg aatagatatt tcaaaagcat
     5101 gtggaagtat gattgtagac gtagggggag gtacaaccga tattgctgtt atatcccttg
     5161 gcggaatcgt tgtaagtacg tcaataaaaa ttgcaggtga caagtttgat gaatcgattg
     5221 taagatatat gcggaaaaag cataatataa tgattggcga acgtacagca gaagaattga
     5281 aaataaatat tggtactgta tatccaagag tacaggaagt tacaatggat gtaagaggga
     5341 gaaatcttat ctctggtttg cctaaaacca taaccgtcac ttccactgaa ataatggaag
     5401 ctcttgagga accaatttct acagttgttg aagcagtaca ttcagtcctt gaaaaaactc
     5461 ctcctgaact ggcggcggat ataggtgaca gaggtattgt tatgacaggc gggggcagcc
     5521 ttatctatgg tttggacaag ctgatacaaa aagaaacagg tattaatgta ttaatcgcag
     5581 aagatgcagt ttccagtgta gccctgggta ctggcaaggc tcttgaaagc attgaagaaa
     5641 ttgaatatag ctatgcttca gaatccaaaa taaaacgcta agaaacatta agaaatacta
     5701 agaaacatta tgaaacgtca tgaaacgtca tgaaacgtta agatcgggaa taaaacatta
     5761 agcctgtaaa tgttaagctt agaaaagaca aaagagtaga tggcaatttt gcagctactc
     5821 tttttattta gttaatgtca ggtaactaca ctattaatta cactatctaa attattcgaa
     5881 tatttctaca attcaatata gctttctaca tagtttcagc agcgatgtta atacatcaat
     5941 aattgggtaa gtatagtttt ggatcttcaa gtacgccatc ttttattact tcaaaatgta
     6001 aatgaggctc aagggcaatt tcgtatgcag cagtatcacc tatagatcct atgctttcac
     6061 cttgagagac aatctgattt gttactacca tatctgcaga agccagattt gcatatactg
     6121 ttttaatccc attttcatga tcaattataa ccgtatatcc cagttttgga tcttccctta
     6181 cttcagacac tactccatct gcaactgcct taactgcagt tcctcttaca gcagatatat
     6241 cgataccact atgggtcctc cagtcttcaa gcgttttgga atataccagc ttatccatgg
     6301 aaaaatcgtt aataattgtc ccatataccg gcattataaa atccttttca attccttctg
     6361 ctgtttttga gtcattactt tttttatcgt ctttattctc tggtgctgca tctttttctt
     6421 cccctggttt tttattatcc tgctccctat ccttcttttt atcctgtttt tcttcctgtt
     6481 tgtcttcctg tttgtcttcc tgtttgtctt taggcttaat tcccggtatt tttaaagctt
     6541 cactgtcatc ggttttagca gctatagtat tatcagctgt agtattattc tcacttgtta
     6601 tgctgctctc cctggaatta ccactcccac ttacctcttc attatttccc agttgtttta
     6661 catctttctc aacttcacta attccagtgg tcttttcggt ctgcaaatca ttactcgctg
     6721 tctgcatgtc ttcatgtcgg tcaacttcat tactgttcaa atcattttca aaatcggtca
     6781 aatagttttc gaaatcatct ggtatgaaat tctccttttc ggatttgagg tttagaaaat
     6841 ccaaattctg tcttgtgata agtactgcag ctccggcgac tatgactact gacaatgcca
     6901 aaataatata gaagcctttt ttattaaaaa cctggaaaat acttttttca gagccttttt
     6961 tttctgaata aaactttttc atactcacag tttccacctc catataatat tgttaccgta
     7021 tatggcaaca atatacatat ggatagaaat tttctgctag tcttctttat ttattttgtg
     7081 gaaaaaatgt acctgttatc atcaattata tcaaggtcaa ctcctgtata gtaatgtttg
     7141 ataatttcct cccaggcaga cccttttttt gccatactgt tagctcccca ttggctcata
     7201 ccgacaccgt gtccatatcc ataggtcata attttaacca tattgttttc gcctttttcc
     7261 agttcaaaat tggctgattt aagtgagaaa acctttctaa actccgttcc ttttaatgtg
     7321 atatttccga tttttatggt ctttaccctt tctcctccag tgtattcaag gatttcaata
     7381 tcaccaaata aatttttgtc attcaatttt atattaggat agcagttttt cagggttttg
     7441 caaaatgatt caactgaaat attggatata gttttaaacg aagggcttcc ttcttcgccc
     7501 tcacttatta cgctttttaa ataatctacc ggacctacat cccaaacatc ttcagcgttt
     7561 tcagtcttgc ctccgctggt tgaatgaaac acagggttgg caatattgcc tttataaatt
     7621 attattatcc ccgctgtgtc aagaactgca ttccttactt tatcccagag ctgatttgcc
     7681 ttatcctgag gccacttttt caaagcatct tctttactga tccaggcctg gcaatgggca
     7741 aaatctgtac atacatctgc attactatgt atatcattac tgtttttata taatccgttt
     7801 agtctgccat atgcataagt acgtgcagca acagcctggg cttttaacgc ttcaacttca
     7861 aactctgcag gcatttctgc agcaacaaca ccggttatgt attcttcaaa acctatttct
     7921 tttactttgt cttctgattc gatatagact tttattttta tattatcttg ccatttttta
     7981 ttttcttcat ccgaaatctg gttatttata tcatttttat tatcatcatt ttcctcatct
     8041 ttatcctgtt tcctgttctg aatctgcccc tgcccgttgc caataacgtt aaagctgcag
     8101 ctttttacaa tgagcattgg aaggacaacg acgattatta tcatcgaaac tatgaaatat
     8161 ataacttttt tcatattaac cccactcagc tattatatta atatatattc tgccggggct
     8221 tatttatgca taatcagtaa cctgtatgtt attccattat gctagtttgg aaacataaca
     8281 aagtagtatt aatcctgtct gtaaatatca acacctaaat ttttaagttt ttcatcaatc
     8341 tttacataac ctctgtctat atgttcaata tcctctactt ctgtcactcc ttctgcggaa
     8401 attcctgcga ttatcagagc agctcctgct cttaaatcag ttgcctttac ctgggtaccc
     8461 ataagtttac tcttaccctc tattaccgca cttcgtcctt caattcttat gtttgcaccc
     8521 attcttttta gttcgcttat atgcatgaat ctattttcaa atatagtttc aactaccata
     8581 cttgttccac ttgccctgga catgagagaa gtcatttgag cctgcatatc agtagggaag
     8641 cccggatgtg gatgagtttt aatatctaca gcctttaatt tcccatccga cactacacgt
     8701 atgctcgtaa gttcttcaga aatttctact cccgcttccg taagttttgc actaacaggt
     8761 ttcaaatgat caggaactac attttccaac actacatccc cccctgtaat agctgaagcc
     8821 gccataaatg tacctgcctc aattctgtca ggtataatgg aatgacgtgt tccttttata
     8881 ctatccatac catttatttt tattgtgtca gtgcctgcgc ctttaatatc agctcccatt
     8941 gatatgaggt atgttgcaag atctacaatc tcaggttcag tagcagcatt ttctataact
     9001 gtctgacctt ctgccagtac cgctgccatt agaatattct ccgtagcacc tacgctggga
     9061 aagtcaaggt atattttagc acctttaagc tttttttcag cctttacttc tacatatcca
     9121 tgtccctgat ttatttgagc acctaaagca gcgaatcctt taagatgaag atcaacaggc
     9181 cttgttccta tggcacaccc cccaggcagc ggcacttttg cataccctgt tctggcaagc
     9241 agagggcctg caataaggaa ggacgctctc attttatgta caagttcata aggagctgcg
     9301 gtgctgttaa tatttgggga agatataaca agagtttttt ctttctcaga taaaacagtt
     9361 tttgaaccga gaaattccat taaattgcac attattacca catcatttag atatggtact
     9421 tcttcaatta tactttctcc atctgctaaa agagatgctg ctataatagg caatgccgag
     9481 tttttggcac cacttattct aactatgcct tttaattctg aagattcatt tataataaac
     9541 ttagccatcg gtttctctcc tcgttcagta tgtcattgaa atagcaggtg tagcaatcca
     9601 tatataagtt ttctgttcat atccattata tcttacagca atgtcaatgt tgcttttgtt
     9661 tctgtcaact ttaattgaat tgccaatagc cggagagtaa gccgacacac tcaccaggtt
     9721 attttgtgca acatttccta ctttttttgc gcctaatata ctaaatatat ttttacatac
     9781 ttcattcata tttatataat caattttacc ctggtaattt cctatgatgc aggaatttgt
     9841 aacaggcttt accttattca ttttaaatgc ggcttcaaca gatttactaa ttaaaggcaa
     9901 tcctgaatag ggaaaacttc cagtgacccc aactgttatt tttctccccg gctgttccag
     9961 tgacttcggc aatccagaca tttcaggtag ctttggtgaa tcagtcatct tcaataattc
    10021 cacagaaatg gatatacttt ctccgctgct tttttcgccg cttattacca gttttcgtac
    10081 taaatcactt tcgtaaacat ccatggatac actattatct tcactactac tatttccttc
    10141 aaggtccaaa ttcaagcttt gggcaatatc attcagcaat ttatttaaat aatcatcctt
    10201 ttcatatttt ttatcaagag cagcagtaaa atatatttcg ctggaaacat atactgcccc
    10261 tgaactttca aaggattcaa tcagtgcttc ttcaggtacc ttatataatt ttcgtttatt
    10321 aaaataatag gaaaatgaaa atactaatac tatgattaat gtaaatatag aacctgctaa
    10381 aatagcagaa tttaaaaacc cttttttcat catgtgagtg catccccctt tctgcggcta
    10441 taatactatt tttgctaaaa ttcaacgcaa aagattctat ataatttacg atttgaaaga
    10501 actaatgaag aatttgcaaa tcgtaagtta catcaaggta acagaagcag gttttaaatg
    10561 ttttaaaacc tgttctatat agtatgatta ttgcccctca gtaaatcgga tatacattga
    10621 aaatttttaa aatggcgtaa aaataagtat atgctgcagc atatacttgt aatatagacg
    10681 tgagctgaat tgtgcatgcg gatatagtta tagctttatt cttattttcc catttttaga
    10741 aaatataaca tcgccatatt ttattatttg atattggttt tctcatctat gtataagtca
    10801 agggctttta ttcctctcgt gacaagtttc acaaataaaa taaataaata tactagcatt
    10861 gctgcacgta ttatctggaa tgctgcagat aaccacatga cagcaccaac tttaccgcca
    10921 atagcatttt ctattccttg gcgataggta tagcttgaaa gcacatcacc catgtgaatc
    10981 cctcctggat ataatatgta aaccagttat aatatctctt ataccatata ataatatctc
    11041 ttataatata atactatata ttggaagttg cggcaagtta attttttgcc gtgttatttt
    11101 ttgctgacag tacatttaga gcatattttc ctcctaaagt acaaaatatg gcagcattcc
    11161 gctttatatg aagaataatt aattaatgag gcttattaaa gtgaattcat aaaaaaatta
    11221 tgacacaaac tcatttatca tcaatacagt gaaattcgta gtttgtgtca taatctgatt
    11281 attgtgttat tatcacacta taattttata atttcaaata aataacctaa aacttactct
    11341 tgtctgccca taagcctaaa gctggattgc ttatataaaa tatttcctct ccatcttcca
    11401 atgtattgta gccatctttc aacttagctg ggttatactt tttatatgct tcacttaaag
    11461 gcatgtattt aaaattagca ccttcaactt cttcctttgt cagcttttca actgcatagg
    11521 ttattgagaa tctgtcatct gatgacccat ggataaggtg tgcggcaact gaaagattgt
    11581 tctgcagatc ggcattttgt tttgtaagct ccagaacctt ctttctccct acatatccgt
    11641 attttctgat taaggcatca tttccgtcat cttctccgaa ttttctgact cctggtgcca
    11701 gtattatcag ttctccatca tctgcaattg ccattcttgt tctgtaaaca gccttattcc
    11761 caagccaggt gctcttaaat tccttctcgt ccaggtaaac aacaacctta ttaaaaggct
    11821 tgtcaataaa ggtgagattc ttttcctggc ttaatttaat tgattcctcg aacacctttc
    11881 tattccttcc aatgaaaacc ccatgtattg atacattacc ctttactgca gttgtaacag
    11941 taaggacata cattaaagga atgtctttaa tgaagttctc ttcagcatag tcaaaaactt
    12001 ttcttacagg agaatggtcc ttccccataa gcttttccat accataaacc gcaccaagca
    12061 tatgagagtt attgatagtt gaacttccgc cgcatccaac aaaaatattc ttgctgtagt
    12121 tggccatacc aataacttca tggggcacta cctgacccat ggatacaata aggtcatagg
    12181 atttatccag caacctcttg tttacctcaa tatccatcgg ctcatttaat agtccctcgg
    12241 aaatttcttc aatatattct ccagggacag tccccacttt aactacatcg gtcctccaat
    12301 tatgaggtat aaagcggtcc attggaatgc catcgccaaa catttctctg cgttcttcct
    12361 cagacattgg ttcgtgggtg cccagagcag gcattatgtc gacctggcag gtatctttca
    12421 gcatctcata atacatagct gttattttac cggcgcctga gtgtgctctt gtaatatcag
    12481 gaggtaaaag gagaactttt ttcaagccag cctttttagc ttcaatagaa cactcaagag
    12541 ccttcttgag ttctatatca tttattccac ttttttcact tgatctcata ataatatcca
    12601 tcataaatat tcattccttt ctaaggtaag acaacactcc tctcctgagc aagaggtatt
    12661 tagtctaaag gttagcggga cacaccttca aagtccgctt caaggaatgt ccccttttgc
    12721 tttatgctat gctttatgct atatatgttg aagcagaagt agagcctccg cgtcctgtcc
    12781 agttggtatg gtagaattct cccctgggtt tgtcaacacg ttcataagta tgtgctccaa
    12841 agtaatctct ttgtgcttgc agcaagtttg caggcagcct ttcgcttctg tatccgtcaa
    12901 aatagcaaag agctgttgta aatgcaggta caggaattcc attggacaat gccgttgcag
    12961 ttactcttct ccagccggcc tgagctgctt ctattttttc tttgaagaat ggatccaata
    13021 atagattggt caattcataa ttcttatcaa atgcttcttt tattttaccg aggaatatag
    13081 accttattat acatccgcct ctccacataa gggcaatacc gccatagttg agatgccagt
    13141 tataggtttt tgctgctgca cgcataaggg tgaagccctg tgcgtatgaa actattttag
    13201 aagcatataa cgcattttta atatcttcaa taaatgcctt tttgtctcct tcaaatttta
    13261 cagccggtcc tgaaagaacc tttgaagctt caactctttc ttcttttaaa gctgagaggc
    13321 atcttgcaaa aactgcttca cctattaatg taagagggat accttcgtca agggctgcaa
    13381 tagcagtcca tttgccggta cctttctgtc ctgcggtatc aaggatttta tcaactatag
    13441 gttgaccatc atcatcttta tatgcaagaa tatcacgagt aatctcaact aaataactgt
    13501 tcagctcacc ttcattccat tccttgaaaa cctcatgcat ttcatcagcc gacataccaa
    13561 gcagatcctt catcaggtgg taagcttcac atataagctg catgtcacca tactcaattc
    13621 cattgtgaac catttttaca aagtgtcctg ccccattttc cccaacccag tcacagcatg
    13681 gagaaccatc ttcaactttt gctgcaatag cctgcaatat aggttttgca tgaagccatg
    13741 catcaggaga cccaccaggc attaggcttg gtcctaaaag agcaccctct tctccgccag
    13801 aaactcctgt tccgatataa aggagtcctt ttgattctac atattctgta cgacgaattg
    13861 tgtccggaaa gtgtgagtta ccgccatcaa taataatatc tcccttttcc agcaaaggaa
    13921 taagcatgtc tataaaatcg tccaccggct tccctgcttt taccataagc ataatcttac
    13981 gtggcttttt cagggatcct accaactctt caagagagta agttccaatt atgtttttac
    14041 ctttagcgcg gccttcaaca aaattcttaa ccttttctac tgtacgattg tataccgcta
    14101 cagtaaatcc tttactttcc atattcatta ccaggttttc acccattact gccaggccta
    14161 taagacctat atctgattta ctcataaaaa aacctccttc tgatttccat aatttttcta
    14221 ttagtttgtg taaattttta tgccttaatc atacattatt ttatattatt tcgcatcagt
    14281 tcatgctatt tcacgctatc ttacactatt ttacattagt tcacgctatt taacgctact
    14341 tttcttccag ggtggtgttg aaggcagata cttgtcaaat tccgcaatca acttttcttc
    14401 ataagctcct gcatactgcc cgttgatgaa tttatccaat gcttcgctgt aaaagcgttc
    14461 ccaggaatca tcttcgttgc cagggtttat aggatagaag agagcattgt ttgttcttgc
    14521 tgcctttaaa tctccaggcg catcgccaat cataagcact ttttcatttt catattctgc
    14581 gcctatggcg aaatgtatgc attcagcttt tgttcccatt tcctggcctg ctaaaacttt
    14641 aacatatttt gaaatatcat gctcttccca ctctcttatc aatgcttctc cgggtgttgc
    14701 agaaactact attatatctg catactcaag cattttttca agactctctc ttataaatgg
    14761 aaatggtggt actccacgaa ctatcttagc tacagtatca ttcactgctt tagaccattt
    14821 caatgttttt gcaagaagtg gatcgccggt tcttctaact tcagtttcca gtgccggatt
    14881 tcccagtttt gtttctccct caatccattt gcgtaaagga ccaaggttag gtactttaaa
    14941 cccgcgtttt ttcacttctt ctctatcagc caaaagatcc ataacattaa ttattgcagg
    15001 gaaacggtta attcctctcc attttgaata aagattaaca aactctgctg cctctctgac
    15061 atacttggaa attgcctgta aatcccattc attaattata ttaggtataa aacattcctt
    15121 atgtttcacc tccattgtat cgaaagcaca tccatcagaa tcaatacata ttaaaaagtc
    15181 attttccttt ttcatatcaa taagctgttg tgcaggatta atcatggtat gtcctcctac
    15241 ttacctattt acatatttac atatttacat atttacatat ttgcatatgc acctgaaaac
    15301 ctgctgatct attaacctaa taactttaat gtttaccctt aacctttaac tttttaactt
    15361 tttaaccttt taacctataa aatactatcc ggttacaatt atacatttgt ttttttgcaa
    15421 ttttctttgc atttattgct tgtattattg caaaaattgc tatataattg tctggtgtgg
    15481 caattcattt ggcatattgg agacatggag gttaatatgc agactcttat ccatgatttt
    15541 acatcaaggc aatacatgat tactccggat tttgaatatt tccattatat tgacagatcg
    15601 gctaatgaaa tcgagtatca caaccacgac ttctatgaga tattctattt tatgtcaggc
    15661 aatgttactt acatgattga aggaaaatcc tatagattaa agcccgggga catactcctt
    15721 atacacaata acgagctcca caagccatta gtggaatcgg gaggtgtgta cgaaagaatc
    15781 gtagtgtggg ttaatcctga ctttttaaaa aagcaaagtc tttataacac taatctttcc
    15841 ttgtgttttg aatcttcatt cccggaaaaa aataatctta taagacctga gccagagatg
    15901 ctggatgttc ttaaagatat tcttgccaaa tttgaaaatg catgtaacag tgtaagctat
    15961 ggcagcaaca tactcaaaga tatttatctt ttagagttta ttgtatatat taataagtct
    16021 tttcttcaat cttttggtga taaacttgat gaagatatag aatatgatga aaaagtgaat
    16081 aatattatca aatatgttaa taaaaatctt aatgatgatt tatccctgga ttccttagcc
    16141 caaaagtttt acctgagcaa gtaccaccta ctaagagaat ttaagaaata tacaggatat
    16201 actattcacc agtatattca ccaaaagcgt cttattatgg caaaatcact tctgaaggaa
    16261 ggcatgtcag ttactgaggt atgcaacagg tgtgggtttg gagattactc aaattttata
    16321 cgagcattta agaatgcatt tggagaatct ccaggtaagt acttcaagga ttaagacctt
    16381 ttcctgctta caactatctg attccttcct ttaaaaaaag actttctgac atcatagcct
    16441 ctcataataa gagtgtcaaa tattttatcc atctgactaa aatggttatc tgacacatcc
    16501 agaataagag tttctccctc gtttatccca cttgatattt cttcaagagt taaattgata
    16561 tcatgattta cctgatttac ctgatttata tcattatcgt ctatattctt atgatacaca
    16621 ctatctcact ccttgatttc tgctacttta tcctgtaaat ttgcaactat gcaaatatta
    16681 tgtgcaatat gttttaaaat atatcgtcaa taacttgtat ataggtatat ataatacaaa
    16741 aacggtccta catggaataa attgaatttt ttgtgtattc gctaaggtag cataagcgta
    16801 caaaactttg taatgtagtt tcttttgtat gttatctata gttataaaaa tattaatgat
    16861 tttataattt tttagaaata ggtgtataaa ctgagaatat cggtcaataa taattaatga
    16921 cctcacaaaa tacattttga ccccctttac aacggttagc cgcaaggcta accctttttt
    16981 tgctttttta ttttttaaca agacaaacat ataaaaatct gctaaaatgt aattatatat
    17041 tttttaacta ttcaaagttc acctaatagg ctgaatacgg aagagttgta tattatttta
    17101 taaccttggg ggttcgtaaa atggttggta tcatcggcaa tattgtgaat actgggaata
    17161 ttatgaatac ccggaatgtc ggaaattttc ggaataccgg gaatgtcgga aataccaggg
    17221 atgacgggaa tatgtcatat aggagtaata acaataataa aagcagcata gttttaatca
    17281 gtataaataa tttaactaaa agctatgcta aaaatttagc agtggataat atttccctgg
    17341 acatatttaa agacgaaata ttcggttttg cggggcccaa cggtgcagga aaatctaccc
    17401 ttatatcatt attaactact ctcattaaac ctgataaggg agatattatc ataaatggat
    17461 acagtgtatt taccgaagcc tttcagatac gaaaaatgat tggatttgta ccccaggata
    17521 ttgctcttta ccccacactt accggttatg ataacctgaa gttctgggga ggcctttacg
    17581 gtctgcgggg ccgggaattg tcagcccgta ttgatgaggc tttagctgta gtagcaatga
    17641 aagaccgtgc ccacgacaag gtagaagaat attcaggagg catgaaaaga cgtatcaata
    17701 tagctgcagc tcttcttcat catccggaca tcctgcttat ggatgaacct actgtagggg
    17761 tggatattat ctccaggcat tacattattg atgctataaa aaatctgaaa agggaaggac
    17821 ggactattat ctacacaagt catgacattg aagaaatgga gatggtctgc gacagggttg
    17881 caatcatgaa ttatggaaga attctaaaat gcgatactgt agaaaaacta aaaagggatg
    17941 gaaaaaatca cacactaaaa gatactgtgc ttaatgtggt cctctctgaa gagcattagg
    18001 caaaaaacat taaaacggga aattaacgtt aaccatttat agtgtatatt atacaaacgt
    18061 taaatacctg cagcacaaaa attcattggg tcattggggt gaaattattt atgaaaacag
    18121 cattgaaaaa aaacacaaat atgcctaata tgtctaaaaa aaatttatat attataatag
    18181 cagctgcttt agttattgtg gcagcagctg tcctggttac tattctttta ttaaattccg
    18241 ggaaaaatgt aaaagacctt tattttcaaa ctgaaataaa aaactttata aaattgatga
    18301 gcgatgctga aaaaagacaa aacgacaaag ctcttgaaat gaagcctttc cttgagaagc
    18361 cctcaagaac aaggcttgaa ttatcggcaa agctgcctca tgggagcagc agcaacgtta
    18421 gtgtaagcag cggcggcaat tccggttatg gttatggaga ccttccgggg caggctatag
    18481 atataataaa cagcagcaag ctggtcctga actcaagata taacttaaaa gaaggactaa
    18541 atgcaagtac cctttcattt cttcttgaag gacaaagttt tctggatata aatacttttt
    18601 cagaaaatga tactattgga cttcaggttc cggtaattta cgataaatat tttgtgcttg
    18661 atagaaacaa tttaccttct gccttaaaaa aattcggcgt cgatatgcct gtcaaaaaaa
    18721 tatttaatgt ttcagaaatt aagaatgcag caaccttctc tctggaggct tccaaaaaca
    18781 tatttacaga ttatctaaag tttcttgaag aaaatattca agaaggtaat atcagtatgt
    18841 ctaagaacgt taaaatgaaa gagatgtcct atcaaaccgg cactaatgct tctactgata
    18901 taaatgcttc taataataat agtagtactt ctggtactat ttcacaagag cccgggaaat
    18961 cagcttccaa ggctaataag gaaagcaggt ataacatctt taatgttaag cttgacgaag
    19021 aggaatttaa aactatatca aagaaaactg ttgaagtttt tttaagtgac agcaggtttg
    19081 taaacatgac tatgggaaat ttgacctcag tatttagtat gcttgaggaa gcagggtatt
    19141 tgaatatatt cccctcgctg aaacctgcag cagcagaaat cctgaactat agagatactg
    19201 aaaagttaaa gactgaactt attaatatta ttgatactac agctttcccg gaaggtttca
    19261 atatgactct tgttgttgat gcggttggta atttcacaga cagaaaaatc aacttatcat
    19321 caaaagccga tgatgaacct gcaagaaaat atagtatcca tgcaggcagg ttctttacaa
    19381 aagcaatcat tgagcaggaa tcatctgtca gtggcaatgc aggcagtact gatgataaag
    19441 ctgttattga atttgagcat gttaagggaa gcaacccggg ggacacatat atgcacctta
    19501 attgtgtaaa caatatatgg cctgacttcg atttaacagt aaattcatca actgaaagca
    19561 gcaaagatga caaaagaaaa gctataaata caaattgcag ggtgaatatg agattgacag
    19621 caagccagct tggttttgac aatgctaata tacttttgga tttgaaacga gaagatagat
    19681 atgatattga tcttaatata cctgaactta ataatgaaag tgcaataaat ttgactactg
    19741 ctgatgaaaa ccaggttaat gcattggtaa tggaacttca gttttcagca gcaaaatttc
    19801 tgctgagtaa ccaatctatt ttaaatgcat tttcatcgga taattaagtc ttggttcagc
    19861 taaaggcatg tgttataaca aaagtggagg gaaatcagtt gaggatactt gttataataa
    19921 aaaatgatat gaaattgttt ttttcagatt ggaaggcagt gatgcttttc tttggccttc
    19981 cttttgcttt tgtcagcatt tttatactgt ccctgtctcc tcttttaaat cagaataagt
    20041 ttattgaatc tttcaatata gcactgatag ataaggataa tacccttgaa tcaagaatgc
    20101 ttattaacca gtttattaac actggaaagg ccgtagaaga cccgaatgat ccggattatt
    20161 caggtacttc tattaaattc ataaaaactg gtgaagcagc agccatggac atgcttaaaa
    20221 atggcgaggc ggcaggaatc ctaataatcc ctgaaggctt tgtatacagc atgtctatag
    20281 gtgagaataa accacttaag gttataaccg acccgggtaa gcctttaata gcagacctta
    20341 ttaaaggtta catggatagc tatgcaaata taatatcgtc agctcagaac gggattatga
    20401 cagcttatgt gttttatgaa aaggctgcct ctggagagga gttttatgaa agaaaatata
    20461 atgatgtagt tacaaagttt tctttaaaag ccctctctaa aagtgatatc ttttcagcaa
    20521 ggaaggtatc ttatgttcca aatgtaacaa aatatgagta ttttaccgct gcactgctgg
    20581 tagtctttat aatgttcagt ggaataatgg gaattaaatt taccgctgga gaaaaacttc
    20641 acggtataag caacagattg aatatttccc ctcttaaaga tatagaattt gtttttgcca
    20701 gatttgtaac tgtgtttata ctatccatgc ttcaattttt agcgcttatt attccagggg
    20761 gattggtttt taaaatatat ctcaacgcat cgccttttta tatgttgatt atgttcatca
    20821 tgacagtttt taccatatct tcatgtgcga ttttcatagc cgttgtatca cccacacccg
    20881 ctacagcaga ccttgcaggc agccttggta ccttgttaat ggcagcagcg ggcggaagta
    20941 tttacccact gacagcattg ccggatgttg taaaaaactt aagcaatttt acaattaatc
    21001 gttgggcggt tcatggcttc ctggaaatat tttcaggaca aatatcctca tctttttaca
    21061 cagatattgc tgtactagca ttgatgggaa taacctacct gggaatgtcc gtatgtctta
    21121 tacgtttatc aggaaattcc aggtgaaacc gctaaataag gcatgcatgc aatattattt
    21181 tctggaggaa agttatgtac aaaatattca tcgttttaaa atacaaatta aaaatactac
    21241 tttcagataa aagttttctt atagcaatga cttttatccc tctctttctg acccttataa
    21301 ccggttatgc attgaaatat gaagaagaga ataagattcc ggttgccata tgtgatcttg
    21361 ataatactga ctattcaaag tctattattg agaggatatc cgcaaaagaa ggatttaaat
    21421 tcattttaac tgatgaaaaa acagctttaa agttggttaa agattataaa gcagaagctg
    21481 catatattat aaagaaaggt tttaaggaaa atcttctgtc aggaaacacc ggtggtttaa
    21541 tagaacaggt atattcgcct tcaacaatat cttcggatat aataagtgag gttataggca
    21601 gtgaagtagc aaggatcatg ctcaatacta ctgctgcaga ttgggtagtg aatgaatata
    21661 ataaaatggg tatcttaaaa gacccaaaaa ttccaaaaga cccaaaagat tcaaaagatt
    21721 caaaaactca gaataatgct ttatggcagg aagcctggga atatacagat tccttatggg
    21781 atcagggacc ggttatggga atagagtatg gtgaaataga tagtacttct aacgggactg
    21841 caggtaaaaa taaaaacctt ccaggcaaca taacatcatc agcctttgga atgctggtgg
    21901 catttgttat gtttctgata atgttcaaca gcagctggct tattgatgaa aaagaaaacg
    21961 gtacattaag aaggataata tcaggttcgg gtgctcttaa tgctttgttt gcaggtaata
    22021 tcctttcctt gctatttgta ggaactttgc agattatttt tttctcattg atatgtacta
    22081 ttgtttttgg agtaaatatc ttttctcaag gaataaatgt attaataatt attctttacc
    22141 ttttatctgt aataggcata agcctcttta tatcatccct tttaaaaaca agaatgcagt
    22201 tgcaggccgg ggcacctctc ttttcaataa ttactgcttt tttaggcgga tgcttctgga
    22261 gctttaaaga tatgggagga ctcataaaga ttatttcttt attgaccccc cagggattgg
    22321 ctcttgacct atttaccaag tacagtgctt cggtctcagg tatctcattt atgactgcag
    22381 caggaatact tatgacaagc tggcaggcta ttgccctgct tattaccgca atagcactta
    22441 catcctttag ttactttaaa ataaaactac tttgataatt tcccatcttc tacagttatt
    22501 attttccata cccctcctgc ttttttaaga gtggcaacaa gagaataaac tagtggttct
    22561 ttatttgggt ctaatgccat actctgagtg tagtgtattt tggaattact tccttgtaac
    22621 tcctctttta acactttttc cacctttaca tttttaaata tttcagtcgc ttcggcaaaa
    22681 gcagaaaata cttcaagggt tatttcagcc ttgcttattt tgaaccccat gaacatttga
    22741 gattcgcttt gaaaattatc atcaaacaaa tcataggctt tttcgtattt accctcacta
    22801 acataagcat aaaaactctt tatcactttg acagcctcac tatttatctg actttgactg
    22861 tcattgccgg tattgttgcc attgctggta ccattactgt tattaacgtc ggaatcggta
    22921 ctgccgctgg taccagtacc attgccagta ccagtaccag tgccggaacc actatcacta
    22981 ccattaccat taccattacc agtgcctgta tcactgacat tgccgctggt gcttccagtt
    23041 ccactgccag tgctgctccc agtaccagtt ccggtactgc cattctcacc agtgttggtg
    23101 ctgccattcg taccagtact ggtactaccg tttgtactat tgtcggtacc agtgccggtg
    23161 ccgccggttc tgtcggttcc gttagttccg ttggttccgt tggttccgtt gccagtgctg
    23221 ctactggtgc cggaaccatt actgctgctc ccaccggttc catctctact taccgattct
    23281 actgtgccat tctgtgagtt ttcagctggc ttggtctgag atgtttcagc aggcttggaa
    23341 gcagtgcttt gggaatattt ctcttcagga taagttaact ggtttttcaa aacattataa
    23401 gttatttcat aactctgctc atcttttacg ggattcatat ccttatcagg aatataagaa
    23461 tattcccgga gttttaaatc ctcctcagat ttccacctga ctatatagta agagtaattt
    23521 ttaccctttt cgtcagaacg attacgtcca atcaactgtc catcttttgt cctgttatca
    23581 acaacaaggc cttcatcatc tgagctgtta aaaatatgaa cgaaagagtt atttccatta
    23641 atatccttta aataacttat aactatatat tccttatcag gtgaaaagac aggagaatta
    23701 agactatttc ccttccattg ataaagaggc ctgatactcc cgtccttcaa tgatagtgca
    23761 taaagattat aagctgattc ttccggcttt aaatctttct ttatacaaaa gtacacttta
    23821 gcatatttgg tgttcaatat agccccggaa atgctgaaag gatttgattt ttcagtgtcg
    23881 gatgttcctg tattatcccc tgaagttccg ctgactcctg aagttacact gcctcctaag
    23941 tttccgctgc cagctgaagt ttctttatcc ataatatcct tcagtccagg gatatcatcc
    24001 tctttataca acctggaacg gcttattcca ttaaggtaat aatctgcctc tataaaaggc
    24061 ttcttattat tatcaaaata caaatatatt tctacatcat attccggaga gttgttttcg
    24121 atagctattt tgtatccatt ttcagttata ctgccatcaa ttgtgctatc atcaattata
    24181 ttattatctg atgtactatc atcagtcata cttccatcaa cagtttcttc ctgttggtcg
    24241 aaagcttctt cagtatcaat tccatctcct gcctttgcct tactattcct tcctgaatca
    24301 gaagatgtag tatgaaaacc gccgggaggg attacccccg tagcaaacaa tgtagtaata
    24361 actaaaccgg ctattactaa aacagatatt aatagtttga aatatttttt attgctgtta
    24421 acacgccttt tattcataat aaaatgcttt ttactcatgc aattattaaa cctccccggg
    24481 gattttaata aattaaagtt tttccatgaa gtcgaaaaat attcgtattt tacttcaaat
    24541 tctcaggatt taggcactga agttcttcag gcacaaaaag tccatccttc cttatgagga
    24601 tatcatcaaa ccagatttct cctccgccat attcaggcct ttgaataaat accagatccc
    24661 agtgtatggc tgatttatta ttattattgc attcatcgta agcatttcca ggagtaaaat
    24721 gtatgctccc gctgattttt tcatcaaaaa gagtatcttt cattggtttc ataatatatg
    24781 gatttaatcc tattgaaaat tccccgatat atcttgcacc ttcatcagta tccaatactt
    24841 tatttaatct ttctgagtca ttagcttcag cttttattat ttttccatct ttaaactgga
    24901 aatgtatgtt ttcaaaggta aacccctgat acaatgcagg tgtattgtat gttatttcac
    24961 cattaacaga atctcttacc ggagctgtaa atacttctcc atccggtatg ttcattttcc
    25021 catcacactt tatggcaggc agtcccttaa ttgaaaaact taaatcagtg cctttccctg
    25081 tgattctaac tttatctgcc ttttccatga acttaaccag actatccatt gctttggaca
    25141 tcttaggata atcgagggta catacatcaa aataaaaatc ctcaaaatac tcggtagcca
    25201 tacctgccaa ttgtgccatg gcgttattgg ggtatctcag cacacaccat cttgtatgct
    25261 taactctaag ttcagagtgc aatggctttg aataatgctc catatatatt ttcattttat
    25321 ctgcaggcac cgcacccatt tcacttacat tttcaggagc ccgtatacct atataagctt
    25381 tcatatcctt cattcttgct atttcataag aagcagttct atctatctgt tcaatagtac
    25441 agttctccaa aagcacacgg tttgtctcag tgtttttaag ggtaataaaa ggtactcctc
    25501 ctgcctcata agtggctttt atcaattctc tggcaagttc atccccttct cccgtggttt
    25561 ctataagaat attttcccca ggcttaatat caactgaata atttatcagc atctttgcta
    25621 atttttccat acgtgggtct ttcatgtaaa tcacatcctc cttaaatttt ataaaattaa
    25681 agcatctttt caaattcaca aagcaatgat tcaaatttct tcatagcatc ttcaataggt
    25741 gccggcacgg ttaaatcaac ccctgccttt ttaagcaatt cgagggggta atctgaaccg
    25801 ccgctgctca agaacttaat atagcggtct acagcacttt tgccttcctc aaggatctgt
    25861 tttgataagg atgcagcagc ggaaaatcct gtagcatatt tgtacacata gaagctggta
    25921 tagaaatggg gaatccttgc ccactcaata gctatttcat catcaatttc tacattgctt
    25981 ccaaaatatt tctcattgag gctgcggtaa atatcacaca agctttgagc tgtcaaagct
    26041 tcaccctctg ccactttatt atgtgccacc ttttcaaatt cagcaaacat ggtctgtctg
    26101 aataaagttg ttcggaaatc ctccagataa ctattaatga gataggtctt ctcttcttta
    26161 ttttttgaat gtgaaatcat gtagtccata agcaggaatt cgttcataat ggaggctact
    26221 tcagcaacaa aaattttata ttcagagtat atataaggtt gtgttttatt ggtataaaat
    26281 gtatgcattg catgtcccat ctcatgagca atggtaaacg cgtcttttat tgtcccctgg
    26341 taattaagca aaacataagg atgtgtaagg aaagatcccc atgaatatgc tccgcttgtt
    26401 ttcccttcat tttcgtatac atcaatccat cctgaattaa aagcctcctt aatatggctt
    26461 gaatactcct gacctaaagg ttcaagtccc ttttcaacaa gagcataacc ctcttcatat
    26521 ggcatattgc tcataggttc tttaaccaaa ggtacataaa catcatacat acagagctta
    26581 tcgaggccaa gagttttctt tcttaatccc atatatccat gtaaaagatg taggttatca
    26641 ttaactgtct ttataagatt atcataaacc tgggatggta cattgtcttc actaagagac
    26701 gcttccagac ttgaattata ttttcttgcg cttgcaaaaa aacggtcttt tcgaatattg
    26761 tttgaaagag atgaagcaaa ggtgttccga aagcttttat acactccata aagtccttca
    26821 taagcatctt tacgcactct tctatcagta ctttccagac attttatata attgcccttg
    26881 gttaattcca cctctttctc attttcatct ttaataaaag ggaatctgat atccgcatta
    26941 ttgagcatgg taaaaatatc agcgggtgct tgtgctattt ccgataccag tgcaagaatc
    27001 tcttcttcct tttgagttaa tatatgatct ttttccctta aaatgtcgcg gatgaaatgt
    27061 gagtaaatct taagctcatt attttcatca ataaaacctt tcagtatgtc tttgccaaga
    27121 ccagtgattt ccggttcgat atatgaaatt gctccggata attcagtcag caaagcaaat
    27181 gctctatcat ttaaagcctg atatttggaa tttgaattat cctcatccct tctcatcctt
    27241 gcatatgcat aaagcttttc tgatatagac aaaatattat cccttaattt aaggcactct
    27301 aacagcttcc ctgatgattc tccaagagtc ccgtttaact ttgctatatc aggaatcatt
    27361 ttcttaacct tatcaaagtc cgattcccat acctcatcgc tggaatatat gtcttccagt
    27421 ttccatttat atttgttatc tatttcactt cttacaagca tattactcct ttccgttgta
    27481 cctatcacca ccctaattct atcgaattgc accgaatatg caccgaatgc gacaagacaa
    27541 cttgtgctca gtttttccat atttgcatat ttgtatgtac ctatatgcac cattattata
    27601 tattattata gtgtatattg ttatattgtt atattgttat acaataatgt gcctgatata
    27661 atacagtaca gcatttatat tttgatttta acataaattt tgccacaatg ctaaatcatt
    27721 aatttattta taacacaaaa tggccctaca tacatagtaa aaggagaaag ctaataatga
    27781 tagcttcttt gcgtcttttt ttggtaactt ttttgagttg acaaatttga actttaattt
    27841 tataatattg gtgttaaggt ttctccctct tttgccgggt gcactgaaaa gagccttatt
    27901 gactaagtta tgcaacggtt ggtcaacaca aaaaatatgt ttccgtagct cagaaggata
    27961 gagcgttcgc ctcctaagcg aaaggccgtg ggttcgactc ccgccgggaa caccatatga
    28021 aaagtaaaat cctagtgttt tttaaaccct gggatttttt aatgtgcagc aaatattcca
    28081 ccgttattat atcaaatata cagattaact taaatttaat tttaattttt tggcataaac
    28141 ttgcaagtat atagcctttg tcaaacttat actatcgttg aaataactcc cagtagaaca
    28201 tgttgtgatt attaataatt tttgtagata acagcttctg attgttatat aatattgtta
    28261 tacattggtg ttacataatt aggttgacat atgagttatg aaatttaatg tttatctttt
    28321 aattagggag gagctccact gtggcaataa acttaataga tttagtaaat ctgatcttca
    28381 acagcagaga ttttataatt gccttattta tattccttct gtatacatat ttatttttca
    28441 tagatagaaa aaagtatata attgtatggt taacaggctg gggtgcaatt ttactgctat
    28501 caattctttg tgctttaaaa tcacaggata tcggcctaat tatcactgct atcttaactg
    28561 atccactgct gcttaattta ttgcttctct ttggagtctt tattcttctt aacggaacct
    28621 atttattttt tgggtataag ttgccgatta tatggatatt agtaacaagt ttaacttccg
    28681 catatttggt tattgtccgt ttttcgtatc ctggttcgta ttctgtttta aatttaccat
    28741 accgcatcat agctgattta ttaacacttt ggattggcat tttgttttta aggaacagcg
    28801 aaattgaaaa agtaagtaaa tacataaccg gcagctcata tatcttactt ggcatcatca
    28861 aactatgttt gccttatata ggtcaatacc ccatatataa aacattgagt tctatttttt
    28921 ataatctgtt gtatattttt attgcactgg gaactttcct gatttttttt gaaaaggtaa
    28981 aaggagattc taatataaca gaaaggaaat tcctaggtgc ctttgaaact tcagctattg
    29041 gaatagccat tgtttctaaa agtggaaagt ttatcgaagt aaataaggct ttgtgcaata
    29101 taacgggata tgcagaagag gaattaatgt ccaaaacctt ccaggatata acctatcacg
    29161 aagacattaa tataggtctt gaatacctca gtcaagtaat gtcaggtgaa atttgctcat
    29221 ttcagataga aaaaagatat attcataagg atagacatac aatatggtct cttttgaatg
    29281 tatcgttaat taagaacaat agtggacagc ccttgtattt agtttcacaa atacttgata
    29341 tatctgatcg gaaaagcact gaagagctgc tgaaaaaaag cgaagaacga tataagcttt
    29401 tattggaatc aattcctgat attgttgctg tcctggacca taattcaaga tataccatgg
    29461 taaacaatgc cgtattaaga cttgttaaca aaaaatccag agaggattta cttgggcata
    29521 aaataacgga tattttaccg ggattcgaag aaacaccata ctataaagca tataaaaggg
    29581 ttatggaaac tcaaaatcct gttactatta ttgataaata cagatatcca cagcaagagg
    29641 gccgcagtac ctggtacgaa gttcacagct tccctgtgcc tgaaggagtt atgtttatat
    29701 catcggatat tacagggaga atgagggcaa tagaaatgct tcgtgattca caagatttta
    29761 tttatgcagt tttaaataca gtagatgctc ttgttcttgc tgcagaccca tatggacgta
    29821 ttatcacttt taaccatgct tgtgaaaaga tatccggcta taagcttaat gaagtgaaaa
    29881 ataaatatat atgggacata cttataactc ctgaggaaat tgaaaaggtc aaatcacttt
    29941 tttttaacat aaaaccaggg gaaactcaag tgaaattaga aaatttctgg atagctaaag
    30001 atggaaaccg ccgcttaata tcatggtcaa acacttctct tttagacaaa aacggatcta
    30061 ttaagcatgt tattgctaca gggatagata ttacagagca aaaaaaggca gaaaggctgg
    30121 aagcggaaat taaggaaaag acaacattat taatccaaac tcttgagttg gacaggttaa
    30181 gaacagagtt ctttgctaac ttgtcccatg agttcagaac accattaaat attatacttg
    30241 gttcaacaca attactattt atgtaccttg aaaatgaatc agcaggaata aatgcaaata
    30301 aattctcgag caaattgaaa tacatcaagc aaaattgcta taggctgtta aggctcgtga
    30361 ataatttaat tgacgctacc aaaatagatg ccggatatct tgaactaaac cctttaaact
    30421 gcaatattgt aaatatcgtt gaagaaattg ccttatccgt ggcagagtat ataaaaagta
    30481 aaaatttgaa cttaacattt aacacaaata tagagaaaaa aataatagcc tgtgaccttg
    30541 atgctattga aaggatactg cttaacctcc ttgccaatgc tgtaaaattt actacttcag
    30601 gtggaagtat ttacatgtac ttattcgttg aggaaaatta tgtgtctatt acggtgcagg
    30661 ataccggtgt aggaataccg gaagacaaac tcggacagat atttgaacgc ttcaggcaag
    30721 tagataaaac acttactaga tcccacgagg gaagtggaat aggattatcg cttgtcaaat
    30781 ctcttgttga aatgcatgga ggtactatat ctgtagaaag cgaatatctc aagggagcta
    30841 aatttacaat tcagctccct gcaaggctta tagaatctaa tgacggtaca ccatatggct
    30901 ataataatca taacaataat aatattgaag gaagttttat cgaaagaatg aatattgagt
    30961 tttcagatat ttacttttga ttctaataaa tttatgattg ttattaattt gtgaatctta
    31021 ttaatttaat attttggtta gcttattatt ccgatttgcg gaaccagtat taatatatag
    31081 tttttaaaac gtagaatatt agcactgcct gcaccagaaa tattaaaggt ataccaagca
    31141 tgaatttaag gtgcctggtt ttatgtctaa atattataaa accaaggtac actcccggac
    31201 accctccgaa aattgaaatc aggaaaaagt acttttcggg aattctccac atcttttttc
    31261 gtgctctatg cttatctgct gctgcggcta caaaaccaag caaattgatt attaacataa
    31321 taataattat tattatactt tctctcactc acatactcct tatcagtagc ttcacgtttc
    31381 tttatacagc ttttattata ccctatacaa ttaataatca caatatgttt tagaacattt
    31441 gtttgcattg ttaaaaatat aaaaaagccc tcacacagct gtattaccaa ctgtcaagag
    31501 ctgtatttta ttgatactat tatttattga aatatctatc taacaagccc ttaaatgcct
    31561 gtccgtgtct tgcttcatcc ttgcacattt catgaactgt atcatggatt gcatcgtaat
    31621 tcaattgttt tgccctggta gcaagttcct tctttccctt gcatgcgcca aattcagctt
    31681 caactcttaa ctcaagattc tttttagtat ctgcatatac aacctctcca aggagttcag
    31741 caaattttgc agcatgttca gcttcttcaa aagctattct tttgtatgct tccgctactt
    31801 caggataacc ttctctatca gcttgacggc tcattgcaag atacattcct atctcagtac
    31861 actcacccat aaaattagct ctcaatccct caaaaacttc aggatcaaca tcctttgcaa
    31921 caccaattct atgttcatca gcccactgca tgttatcacc ctttttctcc acaaacttac
    31981 ttgcaggagc tccacactga ggacattttt caggcggttc atttcctata tgcacataac
    32041 cacatactgt acaaacaaat ttcttcataa aacaattacc tccaaaaccg ttgttttcaa
    32101 atgtgtatat cacgttttat atatttagta tttacccaaa tagataatga attaacattt
    32161 ttttttgcat tattacgttg tttacataaa ttcaacaatt ggcaacctgt ttaatatact
    32221 ggtattagat tgtataaagg aggtatctca tgaattacag agaattactt gaattaacaa
    32281 aaaagagtcc aaatcccttt tccattaatg gcaggaaaac ccgcctttac gattatgaag
    32341 taaggaataa gattccagaa aactcgaaaa agaagaatga agtatataaa agagatagga
    32401 gaagttaatg aacaaaagca gagggtgcta atactaaata caaattaaca gccctggatt
    32461 tagaacacca gggctgttaa tttgtattct tctacttgtc aaagcagtat tgtattagaa
    32521 gtaataggtt gtactataaa attattcttc tgtattaata ttaatcatta attgtcttga
    32581 agtgcattga gcatataatc ccggggtttt attccttcat accttaaaaa tgcccgctta
    32641 aatgaatact cattttcata acctactgca ctggcatcat tacatataat atatacacag
    32701 tcacaatagt tacaacaaat tatcaagtaa tttattctga tagagaatta actgtccagg
    32761 cagtccaaat acaattatag aaaatccaac cttcctttct gtagttttga ttataaatta
    32821 aaccttttct aagtgcaata aacaaaattc tatgcctgag caatattttc taagtactct
    32881 taaaactatg aatttacttg gatttcttgc taattgctat aactcctttg cttgtgttat
    32941 aatttcctta gaaatcagta ttgggctaga aaaactgttt ggcgttgaag gattagaata
    33001 gcattggaaa gcccaaaaca actaataata accaattatt agtccattaa tgaaggaggg
    33061 tagtgtttaa cttggtttcc ggaataacac atgataacta ctggtatatg aatgaagcat
    33121 ataaagaagc cttaaaagcg tataaaaagg acgaggttcc tattggtgct gtaattgtta
    33181 aggatggaaa aattataggc aggggtcata atgaaagaga gctgaaaaat gatgcaaccc
    33241 tccatgctga aataacggct atcaaaaaag cgtgccgcaa gcttggctca tggcggctca
    33301 atgactgcga tatgtatgta actttggaac cttgtgcaat gtgtgcgggt gctcttattc
    33361 aagccaggat aagaaccgta tatatcggca caccggatcc caaagcaggt gctgtaggat
    33421 ctgtagttga tattcttagc atagagaaat ttaaccacaa agtggatgta gtttatggaa
    33481 ttatggaaga tgagtgttct gcaatactta aaagtttttt caaggaatta agaacaaaaa
    33541 agaagtatga cccttgatat atgttttttt gaaataattg tttgaagaac cattagttaa
    33601 aaccctgggt atcataaaga caacgtctgg aatatatttc ttcaaaaata aaatatgcac
    33661 cctgttttca ttttcttgtt gatttgtttc actttaatat gttacaatat ttcagaagat
    33721 attgtttagt ttattgcaaa atcatgccga agtaacaaat tgtactttgt gctggtaaac
    33781 tgaatacagt taggcatgaa tataaccatg aatataatta tggagaggtg tccgagttgg
    33841 cttaaggtgg tagtcccgaa atgtgttggg caatgtggaa atcacgtagt tgaaaccttg
    33901 tatttatgcg gttttcagta ccgtcgaagc tcgaatgaat ttggattttg gtgttgttct
    33961 atccgagttc tatccggggc acttaggtaa ttaaataatt tggagagttg tccgagcggc
    34021 tgaaggagct ggtctcgaaa accagtgtac cgaaaggtat ctagggttca aatccctaac
    34081 tctccgccat catggagctg ttaattctaa agattagcag ctcctgcctt tttaagcggc
    34141 aaagatttct gaataatctt tgctgttttt ttcttggatt ggtattctag atgaccatag
    34201 gtattaaccg ttgtccgaat atcactatga ccaacccaat ctctgatacg ctccataggc
    34261 acttcatttg ccatcatcaa acctacacat gaatgtcgaa ggtcgtggaa tctaatagag
    34321 ggtaatcctg ctttctttaa taaatcacga tgcctgttgg ttatatagtc cgggtcgata
    34381 atttcaccta atggatgaac gcaaatatac ccaagccatt ctttactgta agagttgccg
    34441 caaagctcct ttagttcctt attttcagct attttagctt ttattctttc ttccacataa
    34501 gggatcaagg gataggtgcg gttgctcaaa tctgttttaa gcttatcgga agggatatat
    34561 atcttcttat tatcaatggt tgccgtcgtt acagtgtgat tggccctaaa gaatttgtgc
    34621 ttaaaatcaa attgagattc tctaacgcta agacattcac tgcgccgcat tccataagat
    34681 ccaccgaata atatagggat ttcaataata tcacctacaa taaattcaat ataatattgc
    34741 atctgttcgg cat
//
LOCUS       DAOSDD010000015        34217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_638_length_34217_cov_4.755430, whole genome shotgun sequence.
ACCESSION   DAOSDD010000015 DAOSDD010000000
VERSION     DAOSDD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 34217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34217
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_34217_cov_4.755430"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PK733_02485"
     CDS             complement(<1..845)
                     /locus_tag="PK733_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ09441.1"
                     /translation="MEMMLSVDGGGSKLRMVLFDDEFRLLGWGISGGVNTNHTSLEDS
                     RANVVDCLGQVFGRKPLVHMGRLYVVFVGPVNILYEELAKYATVGDIVALSEARAGLL
                     AGALRDEGILALAGTGSDVFYVREEQALNSVVGAWGPILGDQGSGTWIGQKALRAVVA
                     SIEGWGEPTVIRELIQRKWKLEKYFDMVNIIHRSVAPFRIVASVTPLVGEAAYAGDLV
                     ALQILRKAGLMMAVQTDCLIRRQCIPEQYQRVVCCGGVWKAHPSMFAAFRERLQELYP
                     GIPVD"
     gene            complement(950..3019)
                     /locus_tag="PK733_02490"
     CDS             complement(950..3019)
                     /locus_tag="PK733_02490"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOJ09442.1"
                     /translation="MSTLTFTSYEMPSASLGKLNPLPDLRRTADQHVSIPIDEATITK
                     EEARYMGWGRVNGILPYLLQDGYNRKKKPHAWKAAVLENNFICATFLPELGGRLWSLI
                     DKTTGRELLHRNPVFQPCNLALRNAWVSGGVEWNIGIIGHSPFTVDRMFTEQLALDDG
                     TPVLRMFQYERVRRLIYRVEAMLPDDSRYLFVRVRIDNSSDMGTAFYWWSNIAVDERD
                     DTRVLVPSHKSYRWGYGGKLTKVSIPYMTVSIPYMTVQADQVYGGAGKCGTLHWDISR
                     TTQLPQAMDFFFDIPQGQRRWISAVDGDGYGFVQTSTDVLQGRKLFVWGMGSGGRNWQ
                     TFLAQPGCAYMELQAGLARTQLEHLPMKGGAVISWMEAYGPIAAEANIVHGEDWAAAI
                     GAIEKALECACPRAWVEQMHQRAKLELDDRTGRTKTVGAGWALVQKELLGDKFRDGGL
                     YFPSYSIGHRERPWLQLIREGALPCPGVLEEPGSYQIGSEWEQLLADSIRQGGSRHWY
                     GYYQYGVTSAYAGKLEEAEDAFDQSIQCAVNPWALRCKAVLQDMEGNTEEAAELLVQA
                     VTMLPEHNIAKEALRTLHKAGKNQEVINLYARLPKSVRALGRLKVLLIEALLDTKDID
                     RAERMLNSNIVLTDVREGEVKLTDLWFRMVAIKRSQERGVELDDTLLEQFKKECIPPA
                     HLDFRMH"
     gene            complement(3173..4765)
                     /locus_tag="PK733_02495"
     CDS             complement(3173..4765)
                     /locus_tag="PK733_02495"
                     /inference="COORDINATES: protein motif:HMM:NF023435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ09443.1"
                     /translation="MKKLWITRTFAYLIVMVMIFSLTACSGSTKPSASQGDNSGGNEP
                     TMKELNFVTLDWYLGLDPMPDNQMVNDAVNKYLKEKINANVNMYYWSAKDWETKMTTM
                     ISAGQDVGIIGFGSQSKLDYVVHSNRGSFRPLDDLLNNYGTETKALFPNEIWECMKIN
                     KQIYGIPSLKDNCYIMSFIYNADMADALGLDMGSVKFTNWRQIEPFLTEVLKKRSEKF
                     PEYDQYPLCGGAGREIPYNFALETFLNDSYLAVCNIAPFFDVKGYDDDTVFNLYATNE
                     YREFVKSRQRMVEKNIFAYDYTGKSEWNYKGGMFAWPGWGYTYMQKNLYGDAFTTKMI
                     VSDHIWTETNNYFSAGTAISFNCAEPERAMMILNLVNTDPEFATMMRFGVQGKHYITN
                     NEGKMQFEGSERNGGARADYGYYYWYAAPVGNLTIVKAPESLVGPNGIMLTEIKRYNK
                     EAVMPNHMGFVFDIKPVANEIAACTNVVMEYRDILRNGQLESEKEVDKAVDEFVAKLE
                     ANGVKKVLAETQKQINDWKASK"
     gene            complement(4830..5753)
                     /locus_tag="PK733_02500"
     CDS             complement(4830..5753)
                     /locus_tag="PK733_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09444.1"
                     /translation="MAKEVKRVNKLTLLSKGWNAVFVIILFFMALLVVIPMLLILIVS
                     LSSEISIADYGYTFFPSEWTLDGYRYLFKTGDQLADSYLVTIFYSITGTLMSLTVMTM
                     FAYVIAQKKFKHSRLFTWLLFFTMLFSGGLVPIYILNVRYLHINNSIWIFLLPSLVNA
                     YYTVILRTFIRTTIPDTLFEAARIDGAGHFRIFVSIVLPLFKTGIATVGLFNMVSRWN
                     DWFTGMLYIQNPKLVPLQTLLTKLQNTIDFLKQNAAIAGTPDGIALLRTLPDENMRMA
                     CTIIVILPILFAYPFFQRYFVQGLTMGSIKG"
     gene            complement(5781..6728)
                     /locus_tag="PK733_02505"
     CDS             complement(5781..6728)
                     /locus_tag="PK733_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09445.1"
                     /translation="MLQTRANVRLKKRKKIFQKEDAALYLMALPTLIYLLIFCYVPMG
                     GLVMAFQDLNITKGIFRSPYVGLKNFEFLFRTTDAWVITRNTVCYNLIFIVVNLIISI
                     SLALMLSSLRSGGYAKTMQTIYMMPYFLSYAVVAIVANAFLDRDNGLVNSILRLMGEP
                     GKTNWYHQLRIWPPLSVFVNAWKNVGYQTVLYLAVISGISVDYYEAAVLDGATKFQQA
                     CYITLPHLRYIVGISLILSMSSIFRGDFGLFYNVPMNSGKIYPVTDVIDTYIYRSLTY
                     LNNVGMSTAAGLYQSVVGFVLVLVVNKIVNRIDPDSAMF"
     gene            7241..8989
                     /locus_tag="PK733_02510"
     CDS             7241..8989
                     /locus_tag="PK733_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOJ09446.1"
                     /translation="MRLQVSRSKNAASFYVTKSVYDKGKRSNKIVEKLGTYAELKAKL
                     NGRDPYEWAEEYVKELNRLEKEGREPDVIARYSPYKRIEKDVRCSFNGGYLFLQQIYH
                     ELELHKLCKDISQKYKFKFDLDSILSRLVYGRILFPLSKLATFEESKKFIEQPSFKLH
                     QIYRALEYLAKETDFIQSTLYENSLKISRRNTGILYYDCTNFYFEIEEAKGKKQYGAG
                     KEHKPNPITQMGLFMDGDGIPLALSINRGNMNEQLTLKPLEKKILSDFELSRFVVCTD
                     AGLSSEENRKFNDKDGRAFITTQSIKKLKEHLKKWSLASTGWKLPGDEKTYDISKLDE
                     MIEKASAKDKEKIRAKVFYKERWIKENSLEQKLIVTYSVVYRDYLCNIRNSQIERAQN
                     TIDDSPEAIKKCRQNDYKRFISKTNITIYGEVADKEIYGINTALIQKEEAFDGFYGVC
                     TNLDDDVSEIIKINQRRWEIEECFRIIKSEFRARPVYLSDDDRIEAHFITCFISLVIY
                     RLLEKRLHEEFTCHEIISELREMNFKEIKGEGYEPIYIRTDFTDALHEAFGFYTDYQI
                     VTSKQMKKIFKATKSK"
     gene            9339..11678
                     /locus_tag="PK733_02515"
     CDS             9339..11678
                     /locus_tag="PK733_02515"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ09447.1"
                     /translation="MYSLKNHIVYLSSKRSVLITLFTSFIILTLIPLIIWGFFYKKTE
                     SIMKENVNRYNIAMLEQVKHIMDGNLQETERTAYQISFSSKLHNIISIDYPPDVSNTY
                     QYIDFKNELAVYKRTNTFISDFYVYFPKDDVILMPDMKTNSSTFYDNFYKYMEIDYNS
                     WIEILNSYHYKYYLPVKEILAYSKPQKVITYIQSLPFGNKKDIKACLVIFIDESKVKK
                     LLDNVKWATNGYICIINKDNQVIMSTGNILDNTDIPYSEFNNDVSVYKDKSGSEIMVY
                     CSISQESDWKYISILPKNEFMEKVNNMKRWIQNNIFFYLVLGLMLAFFLTYKNYNPVK
                     NLISAIFSEKKIKDKNIQNEYDFIKQSILDGMSENRKLSDIIYRQMPAVKSNFLMRVM
                     YGIIDYSTITKESLKFIDIDFVSDYFMLLIIEIENFGSIFSQDSENEKALARFALTNM
                     GNELVNEYGKGYLIELESSRLALLFNVFEERLDKAESDLLNISKVLRNAAQEHFDVFI
                     TIGISSIHKGTFGIGESYKEASRAIDYKFVKGIGEIICFNEIESVKQNYYYPLESELI
                     IINTVKNGDYNSVEKILDKIYNDNFKPYNVAPEHGKSLFFNIMSTLVRIMNTTNIKFE
                     NILTGETDIVNYLTGCSTIEEMHEKIKKAFKLICLYIDSNWNNRKNHLLEQIKNYIAK
                     NYTDNTLCLVSIADNLNITPQYLSTYFKNANGENLSDYIAKIRVGKAKQLLAEGQLTV
                     FEIAKSIGYTNDVGFIRVFKKIEGVTPGKYKSMISSEHK"
     gene            11979..13649
                     /locus_tag="PK733_02520"
     CDS             11979..13649
                     /locus_tag="PK733_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09448.1"
                     /translation="MRNNIIRLIALFVSVFLVMLLFSGCGQTSKNGNTSNQTTASSAA
                     DSITGQEKNNTLIVDKPLTLTYYVANSARGQVQDYNEILAYKEMEKKTGIHIEFQHPV
                     ANTLEERVEQFNLMLASGEYPDIISVAWSEMKQTPEQLIDDNVIIGLNELIDQYAPNY
                     KKILDSNSDIRRGAVTDKGRHFAFKNVYNHESTAVWFGPIIRKDWLDNLSMDVPVTID
                     EWYTVLQAFKSKDPNGNNKADEIPLLIGAFGDPLGSFRLGRAFIGAWGIAMEYYQDNG
                     TVKYGPLQPEFKEFLKVMNKWYIDGLIDRDYAAMKTELLVANVTNNLLGSLTAGASGA
                     IGRYTNMMKDKDSKFKLVGTPYPVLKAGDKPQLGQRDSMVLNIGAAITTKCKNKIEAV
                     KWLDYAYSEDGNMLMNFGVEGTTYTMVDGYPKYTDLILKPRDISVDENQVKYCPTNMG
                     GPYTLDYRSYEQLISLPEQKDAIKIWAATDTSKLIPDNVVISPEDLAKNATRETDIKT
                     YMDEMINKFIMGVEPIENYDKFVEKIKTLEINSLIAAKQKALDNYNKR"
     gene            13877..14869
                     /locus_tag="PK733_02525"
     CDS             13877..14869
                     /locus_tag="PK733_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09449.1"
                     /translation="MNTTNIKQTRAKQTRTEFESGLLKTKLKTKLKILYDDLKRNKLI
                     YLMLIPALVYIIVFRYVPMYGVQIAFKDYSFSRGIWGSEWIGLRNFRDFFTSIYCWRV
                     IRNTILINLISLFFAFPIPIILALLLNEVKCMAFKRTVQTITYMPYFVSLVVTVGIIY
                     DFFSNNGIVNSMLSYLGIEPIQFLQKPQWFRPLYIGSGIWQEAGWNSILYLAALTNID
                     PTLYEASDLDGANRWKQTLHVTLPGIMPVIVIMLILRMGNMMNVGIEKIILMYNPLTL
                     ETGEVISTYVYKRGIQEMSYGFSAAVGLINSTINLILLTASNWMSSKLSENKLW"
     gene            14902..15780
                     /locus_tag="PK733_02530"
     CDS             14902..15780
                     /locus_tag="PK733_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09450.1"
                     /translation="MKRRTSGSIIFNVFNYLFLALISMIALYPLVFILFGSISEPVLI
                     AQHRGLLLKPLGFSMQAYKIVFDNPMVALGYRNTLIYLVLGTTINVALTSLAAYALSR
                     RNILLGNFIMFIIVFTMFFDGGLIPTYLLIKRLNIYNTIWAVIIPNAMRVWCFIIMRT
                     GFQQVPAEMEESAKLDGANDLQILYKIIIPISMPVVAVMILFYGVDHWNSYFNAMIFL
                     RDRKLFNLQLVLREILIVNDTTKTTARFASEDRYAVQISIKYALIIVATVPILLVYPF
                     LQKYFTKGIMVGALKQ"
     gene            15930..16127
                     /locus_tag="PK733_02535"
                     /pseudo
     CDS             15930..16127
                     /locus_tag="PK733_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            16271..16696
                     /locus_tag="PK733_02540"
     CDS             16271..16696
                     /locus_tag="PK733_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09451.1"
                     /translation="MALNWCYNEPWPCVANNSLLSYPAAPKKAYHAAAQSCRPVLLSA
                     RLNRFSYKADDLFEAVIWLLNDSPVDIEPGKAIVTLTLGKNSYNLTEWEYGKAKAGQN
                     IEGPRVSMQLPLAAADCLILTINAGEYSSEYRLLYRLGA"
     gene            17088..18488
                     /locus_tag="PK733_02545"
     CDS             17088..18488
                     /locus_tag="PK733_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ09452.1"
                     /translation="MFNKTDKLHDTNKLPMTDIIDTTDKSHRTSSITQNLYMKAKKII
                     PGGTQLLSKRPEMMAPGQWPAYFSKAKGCEVWDLDGKHYYDMATNGIGACILGFADPD
                     VNEAVRCRIDMGSMCTLNPPEEVELAELLCDIHPWAENVRFTRTGGETGAVAVRIARA
                     TTDRSVVAICGYSGWQDWYLAANLGESDALRGHLLPGLDPVGVPRELRNTTVTFRYNN
                     KEEFKSVIDKYGNRLAAVVMEPVRYNDPEPGFLEYVRDGTHKCGALLVFDEITIGWRL
                     IYGGSHLKLGINPDIAFFAKSLGNGYPMGAVIGTREAMKGAHLSFISSTYWTESIGPV
                     AALAALKKMKEVKAYEHVSKIGTLVMNLWEINAQKHGVEIDVGKGYPCLAHFTFKNDF
                     DNKLKTLYTQLMLERGFLAPTSIYPTVAHTEEIVKKYGDAIDEVFYLIAEAKRNEKVD
                     NLLKGPVAHVGFARLI"
     gene            18864..20540
                     /locus_tag="PK733_02550"
     CDS             18864..20540
                     /locus_tag="PK733_02550"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09453.1"
                     /translation="MYRLMLVEDEEIICHGLKNKIPWKEVGFEVVGEANDGETALRMI
                     PELWPDAILTDIRMPGLDGIGFIEKLRELGSKAEVVVLSGFDDFEYARKCIAFGVTEY
                     LLKPIKNDVILSTFYQLKCKLDSRYSKDSEFNNIKKKIELTIPVAKGKIIQDILENRL
                     NKEDTDRIHAILDMPFDKTASFGVAVFHISRLMNTKITFDDGSRDCFQRVLENETARL
                     PQYQLLSVLFQKDTYTIVAVWSVHRRQKEDTGRDLCNAIREKLENLPAYTGKILVSVG
                     VGSIFDNIFGLSRSYIQAKNALMQKFYIGLGKVISYTEIQNEPGRTKVQLLDKTIDSN
                     QLVRKIIEYIVSGDMISSEKSVKCYCDTYLGMRRYNPEILFMRVIELVLKLAGALEEQ
                     GISFTDVYGEGVDQEIRFLVEEKTIDELREWLIMLANRLIKQSERTEDTYSIDHMIEV
                     VKQYVQKNYNRKITSKELCDIAMVSPSYFSSMFKQKTGYTLMEYVIMVRIQKAKELLA
                     KSDYKIYEVATVVGYDDFRHFSKQFKKLEHISPSKFRKDNEEKLLNRNRL"
     gene            20651..23488
                     /locus_tag="PK733_02555"
     CDS             20651..23488
                     /locus_tag="PK733_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ09454.1"
                     /translation="MKKPKRKNTMSVRLFLNLTAALLCFLFLVIEGIISFGVYKNIVD
                     LKVGEIYQNQLQQVTDRISFELRLIENLINGIVQNSEQNRFCCRYQEEKNMYTKNQLQ
                     KEIKNDLNERTIGLKVSGFEIFLEDNSTMMQNKQIMLANFAPEYKSLHSKLSQSERNV
                     VLIPTLDDESPWIDRNNHIMFAAALTEGGEEAGILVLQMDSTWIDSMLGVGSNAMLFE
                     RENQVILGGFMYENTFQAKPIFEKIDGENGSFTAHTEQGDWKIQYQNIEGSSLTAVIP
                     KSISELFSAIKNVLKLVIVFTLLSSVAGFALGELLVGKTVRPIHTLLHEVKSLGAESA
                     GPGKEEQRNINSSMRNTVFLYYNVVILIPMLVYIMTFYFGASNNIEHIVKDSLTQVVE
                     KIVHDIAVYMEVNESASIKIVMDSTVQHFLTEHEEKMSEITLSDIVKLDKVVKKSMTF
                     AGTTTNVEIFDSKGIRVYAGNDYETGSKISANYKDMAVKSRGDAVWNYTDIDRYGKRM
                     ICLIRRIYDLCMELPPFRTIGYIKLTIPETEIKNLYYNIKYDNSDFFVLDKKGLYVSG
                     RNTARIGEPSGYMGYIGDGIFAGSRNRKVVRNNKLYDIITAEDSRMPLAFVGEISRDD
                     IIKNNNLIISSSLYLILTICIVIFILSYSISQFFANAFNNMTHKLIRFFKGDLQVDFS
                     SHFYFSEIGQLADTFNHMSGRINDLTETLYNVHLKAKELEKEKKESELIALQAQINPH
                     FLYNTIESIKWMMKYNNESAETMLTKLGELFRLGISSKVQLVTVAEEIRYANTYIDIQ
                     RLRYKDQVKIIWEIDENVYENTMPKFTLQPIIENVFKHAFRFKKENCIIHICGYLEEN
                     SVVFSIMDNGDGIPQDRLYEINELLQSDLPGESMGIYNVQKRIRIYYGDQGGINFISQ
                     EGVSTIVKVCIPVEKNNDNNIQNMQNSTQLQ"
     gene            23613..25358
                     /locus_tag="PK733_02560"
     CDS             23613..25358
                     /locus_tag="PK733_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09455.1"
                     /translation="MKSSYKLVSILLTLMLCATMFAGCTFTTTQKDDKTATEGENKAT
                     VTVKPKVDVSLELVFLPTTLAEDQSSNVIAKYIEDKFNVSLKFKPSDAEKQRLHLSTG
                     QAADIISLAPWLGTTDLITKGVEEEIFVDIGKLVNAEPGRYPTLAKMFKEPLYQYFNG
                     YYYGKPKEFYSMYALDYTRNVAGAPIYNKKIMDELGLKIPKTVDEFVEYLRTVKAKKP
                     NIIPFFFRNDKGSWLTGSSGEINQIFFQTYGLNASSVQKDKDGKWRDTSIDEANKVVW
                     KRLAGMYAEGLIDQEVFTKEADTFMANDFVTGKIAVAVSHLPNGNPNSALYPYDEFKK
                     GNPDIDPATFLDYVVYQPEPLTGPAGHGKTRAVAKSVPFCSFITSTCKNPDRALDILN
                     WIISDEGQTVLNYGIEGVHYKSLDAKGRVVGYNLEKFKEDNMGWGFGGLTFGFLLLSG
                     SGTRYLPDRYGWSESVMHNENLAEGGFSTELPKNLADYGTEIYNKWSTQCYEELPTYY
                     NYISFTDEFKQIGKKVNEIKVKYFVGFIFGTYDVDKDWNKFVQEFEDAGGSKYAAEYG
                     RLLDIEKEKFEALGK"
     gene            25615..26559
                     /locus_tag="PK733_02565"
     CDS             25615..26559
                     /locus_tag="PK733_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09456.1"
                     /translation="MEVTLKKQDRESFIERLKYNIKMYWFIYLLVLPGIIHLIIFRIS
                     PIYGISVAFKDYKISKGILKSPWVGFDNFKILFTDKYFYKVIWNTVLLNIYSIIFGTA
                     FTIFLALMLNEIKNQFLKKTLQTSVYFPNFVSWVIFAGIVNAMLSPADGAVNLILKLL
                     GLKSIYFLGSNKWFRFIIVASGIIKTAGFGTIIYLASIAGINPEIYESASIDGAHRGQ
                     LLWHITLPRIKPTIAVMLIMSVSNIFGSNFEQIYNLYSPLVYETGDVLSTYMYRTGLL
                     EGKFETSTALGLAFSVIGLGIVIMTNKVVNKMDVMGFF"
     gene            26698..27594
                     /locus_tag="PK733_02570"
     CDS             26698..27594
                     /locus_tag="PK733_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09457.1"
                     /translation="MNMKHKLGFNFYDVFVYAFTIAFAIFCIYPMWYVFIGSITPSAS
                     SAQQPLRILPPHNPTLEYYNIVLHAKEFKHSMFISFLKTIVGAIGSLIFTAMMAYGVS
                     KRQVMGMKTLNFIIVFFMFFSGGLIPTYLLYGRMHLLKTFWVMVIPGFISIGHFVIMR
                     NYFCYNVPEDLESAAVIDGANNIIIFFKIIIPISMPMFAAIFLFEAVGHWNDWTSYLY
                     FVGEVKYMPFIVVLQEILRNPNGQIMRDATATVMNIQQRTLPPASLINTTIIVAMLPI
                     LVIYPFLQKHFAKGILIGAVKG"
     gene            27667..29886
                     /locus_tag="PK733_02575"
     CDS             27667..29886
                     /locus_tag="PK733_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09458.1"
                     /translation="MGDKLIDMNKKQPKKASVVIRSNSENVIRFAAEELCGYLEKLFD
                     VDASISEESPENAEILFYVGTQQENPYFLENGISDKLEASSKQVVMSKQVVMSEQVVM
                     SQQVVMIRRVSENSMVLSGGSPVAVLWAVYGLAAQWGVEFLLHNDVLPEKCTLYLPDV
                     DICEEPLEPVRTWAFPHGLAFGPESWGISDYENILIQLAKLRFNRIYMQFYPEFPLID
                     LEIGSIRRSTTGLFFNYHYPITDDMIGRDLFDDRSEFWNRDLPVEANCAEMLAAGMKL
                     VHGVISIAHKFGMEFIMGVLPLEYTREFAPILKDPLYVKLLNTETVVPGASIKVEDDQ
                     LFEMALAVVRSVVDNYPDIDIIAPVMPEFRDWVEYSETAWQLLNEKYDIEEICTYNEV
                     MEKAARRTEYPGGGARAVKEVKGDIVALYFYDLFFREKRAMSYTCKPDMKLMYMYLAE
                     ELFPLLTRILPEGTEITCFVDYTPTRILKRCEALGTIQPETIYTLCYTLHDDNIGFLP
                     MVAAESLGKLLTTARKAGWKGFMSRYWLTSDHDICINYLANAMWNSSQSLQAAYWRLI
                     SAMCGNDCVYELSNMYSELENATVILEREGLGFAFPVPDMVSYHWGKKEPSKNMDQVR
                     LDYEQALDWAQKARPKAAEKGMWFVDYWINRIMFGIKYLQMTEAVFEGGQVAAKGYIT
                     EAKAYVKHALDLAEEACISYAKAARDRSDVASIAIFNEYGIRYLKKKLEDLNSQTKL"
     gene            29942..31195
                     /locus_tag="PK733_02580"
     CDS             29942..31195
                     /locus_tag="PK733_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ09459.1"
                     /translation="MHIHGGIIMNNIILSAPLTHSDWYLKTNGPEWGTEGIRTMLTRC
                     KEFGFSRIYWRVYDSGKATYASKLVEPFRADEHEEIWQYSPGYIDLPPEETMKRFMQL
                     DYTNFDSLSAAIEIGHEIGLEIFAWMSINEDDHGIGFTSKFTRENPQYRWICRDGRSY
                     HSQLSYAFSEVREYKLALIREMLAYDIDGLFIDWIRTGDIRDNPQSDVDGVADYGYEK
                     PNIEAFIQEFGIDPRCVPNNDERWIHCRAKPITEFMRQARALTKSHSKKLRLITMVHH
                     PWSYRGVMPEMINEDTPQWVRNMSGNRVDGALNGLLCDIKTWSKENLMDDVIAAGYYI
                     KGGNALLACEYLKKETEGKTPIMVYGWVPTTPEAFYKDVEIAQQVGTNEILYWEADYI
                     DNSGADFRASHFSRCVSSQAPNTTQ"
     gene            31228..31485
                     /locus_tag="PK733_02585"
     CDS             31228..31485
                     /locus_tag="PK733_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09460.1"
                     /translation="MASSRNGAKVILIEKQCALGGLAILGLITIYLPLCDGMGKQVIY
                     GIGEELLRLSIKYGYEDNYPNAWLDNNNIEERKKTAFSCAI"
     gene            31724..31969
                     /locus_tag="PK733_02590"
     CDS             31724..31969
                     /locus_tag="PK733_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ09461.1"
                     /translation="MKGTGIVREVDELGRIVIPKELRKVMDIKVKDPIEIFTEGSAII
                     LKKYSPGCIFCERVNDVIRFKENNICTDCLKQLKNDD"
     gene            complement(32605..33063)
                     /locus_tag="PK733_02595"
     CDS             complement(32605..33063)
                     /locus_tag="PK733_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09462.1"
                     /translation="MFESLEIPDKDILPTYLHNCLYVYPQRICDVYVTNALVLLASLL
                     YFSPNLVAKLADNHVDNNADNDADNDAGNSANKPADNPSGNPTGNSSDNLAGDLPDNP
                     PDSFSPSVYSIFPVSHQVLHSLKYMYCQYQMSICGNIPFSMYNIHFSNGL"
     gene            33425..34216
                     /locus_tag="PK733_02600"
     CDS             33425..34216
                     /locus_tag="PK733_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOJ09463.1"
                     /translation="MKVLLMTDLEGISGVDTMDKVSDVGTPGYRFALERLMLDVNAAV
                     EGAFEGGATAVYVKDGHGGGNNFLKEMLDPRAIQVSDSISWQRLLKDGEIDAYMEVGA
                     HAMAGTINGFLDHTQSSVNWHNYIVNGRKSGEIAQGAIFAGAFDVPFVMVSGDEAACV
                     EARAFLGNIECAVVKYGIGRNNARLVDLDEALEKIKVAARDSLKLVGKIKPYKPLLPL
                     EIILELNRSDMCDALMERCKDVERLDARTVRKLVYKVESYIDILF"
BASE COUNT        11114 a         6022 c         7127 g         9954 t
ORIGIN      
        1 ttatctaccg ggatgccggg atataactcc tgcagtcgct cacgaaaggc agcgaacatg
       61 ctcggatggg ccttccaaac acctccgcag cacactacac gctggtattg ctccggaata
      121 cactggcggc ggatcaggca atcagtctgt accgccatca tcagaccggc cttgcgaaga
      181 atctgcagtg caaccagatc tcctgcatac gctgcttccc caaccaaagg cgtaacagaa
      241 gccactatcc tgaagggagc cacggatcta tgaatgatat tgaccatatc aaaatatttc
      301 tccagcttcc acttccgttg aatcaactca cggatcacag ttggttcgcc ccaaccttcg
      361 atggaggcaa ctaccgcccg aagggccttt tgtccaatcc atgtcccgct tccctggtca
      421 cccagtatcg gtccccaagc acctactacc gaattgaggg cctgctcttc tcgcacataa
      481 aagacatccg aaccggtacc agccagtgcc aggatccctt catctcgcag cgcacccgcc
      541 agcagcccag ccctggcttc actcagcgca acgatatcgc ccactgtcgc atattttgcc
      601 agctcttcat atagaatgtt cactggccca acaaacacca catacaacct gcccatgtgt
      661 accagcggct tcctgccgaa tacctgaccc agacaatcaa caacatttgc acggctgtct
      721 tccagagaag tatggttagt gtttaccccg ccgcttattc cccaaccaag cagacggaat
      781 tcatcatcga acagcaccat tctgagcttc gagccgcccc catcaacgga caacatcatc
      841 tccatataaa catatcacca atcctcagaa acatatcatc aatcttcaga aacacatcac
      901 caatcctcag aaacacacat caccaatcct tctagtgcta tacgatagct taatgcatgc
      961 gaaaatccag atgagcaggt ggaatgcatt ccttcttgaa ttgttcaagc aaggtgtcat
     1021 ccagctccac tcctctttct tgtgagcgct taattgcaac catgcgaaac cacagatctg
     1081 tcagcttaac ctcgccttcc cgcacatcag tcagcacgat attactattg agcatgcgct
     1141 ccgctcggtc aatatctttt gtatccagca gtgcttcgat cagcagaact tttagtctgc
     1201 caagcgcgcg tacactttta ggcagcctgg cgtacagatt aataacctcc tggtttttac
     1261 ctgctttgtg cagagttcgg agtgcttctt tggcaatatt gtgctcgggc agcatggtca
     1321 ctgcctgaac caacaactct gctgcttcct ccgtgttacc ttccatatcc tgaagaactg
     1381 ctttgcagcg caatgcccaa ggattgactg cacattgtat ggactggtca aatgcatctt
     1441 ctgcttcttc aagtttaccc gcataggccg atgtgacacc gtattggtag tagccatacc
     1501 agtgcctgct ccctccctga cgtatggaat cggctagcaa ttgttcccat tcactgccga
     1561 tttgataact acccggctcc tctaatactc caggacatgg cagagctccc tcgcggatta
     1621 actgcaacca tggcctctca cggtgtccaa tgctgtacga tggaaagtac aaaccgccat
     1681 cccggaattt atctcccaaa agttccttct gaacgagagc ccaacctgca cccactgttt
     1741 ttgtcctacc agtcctgtca tccaattcca gcttagccct ttggtgcatt tgctccaccc
     1801 aagcacgggg acaggcacat tccagtgctt tctcgatggc cccaatagca gcagcccaat
     1861 cttctccatg cactatgttg gcttcagctg caatcggccc atacgcttcc atccagctga
     1921 tcacagcccc acccttcatg ggaagatgct ctaattgagt gcgtgccagg ccggcctgaa
     1981 gctccatata ggcacagccc ggctgtgcta aaaaagtctg ccaatttcgg cctcctgatc
     2041 ccattcccca aacaaataat tttcgtccct gtaaaacatc agtcgatgtc tgcacgaagc
     2101 catacccgtc tccgtctacc gcagaaatcc accgtctctg cccctggggg atatcgaaga
     2161 agaaatccat cgcttgcggc aactgtgttg tgcggctgat atcccagtgc aatgtgccgc
     2221 atttacctgc cccaccatac acttgatcag cctgtaccgt catatacgga attgacaccg
     2281 tcatatacgg aattgacact ttagtcagct ttccgccata gccccatcgg taggatttat
     2341 gggatggaac cagtacacgc gtatcatccc gctcatcaac tgcaatgttg ctccaccaat
     2401 agaatgctgt gcccatatca gatgaattat cgatgcggac ccgcacaaac agataccttg
     2461 aatcatcagg cagcatggct tcaaccctat agattaggcg gcgcacccgc tcgtactgga
     2521 acattctcag taccggcgta ccgtcatcca acgccagttg ctcagtaaac attcgatcca
     2581 cagtaaaagg actatgtccg atgataccga tattccactc tacaccgccc gaaacccagg
     2641 cattgcgtaa agctaaattg catggctgaa agactgggtt gcggtgcagc aattccctgc
     2701 cggtcgtctt gtctataagg gaccaaagcc ttccacccag ctccggcaaa aatgttgcac
     2761 agataaaatt attctccagc acagcggctt tccaggcatg gggcttcttt ttacgattat
     2821 agccgtcctg cagcaaatag ggcaatatgc cattgacccg cccccagccc atatatctgg
     2881 cttcttcctt cgtgatggtt gcctcgtcaa tgggaattga aacatgctgg tcagctgtcc
     2941 tgcgcagatc aggcaatgga ttgagcttac ccaatgaagc cgagggcatt tcatagctgg
     3001 taaatgtaag tgtggacact gttttgcact cctccttcat aatatttcat aaaatggccg
     3061 ctcactcgaa tcagaccaga gcgaacgacc attcaatgtt ggagaaccta tttttcttct
     3121 agtcgaggtg ggggacatct accgcttcat taaaattata attactttat cattacttac
     3181 tggctttcca atcattgatc tgcttctgcg tttccgcaag tacctttttg acgccattgg
     3241 cctcaagctt tgcgacaaac tcatcaaccg ctttatctac ttccttttca gattcaagtt
     3301 gcccattacg caaaatatca cggtattcca tgaccacatt ggtgcaggca gcaatctcat
     3361 tggcaactgg tttaatgtca aaaacaaaac ccatgtggtt aggcatgacc gcttctttgt
     3421 tatatctctt tatctcggtc agcatgatgc cattgggacc aaccaggctt tccggtgcct
     3481 taacgatggt aagattgccc actggtgcag cgtaccagta gtagtaacca taatcggcac
     3541 gggcaccgcc gttgcgctcg ctgccttcaa actgcatctt gccttcgtta ttggtaatat
     3601 aatgcttgcc ctgtacgcca aagcgcatca tagtcgcaaa ctcaggatcg gtattgacca
     3661 aattgaggat catcatagca cgttcgggtt cggcacagtt gaatgaaata gcagtgccgg
     3721 cactaaagta gttatttgtt tcggtccaga tatggtcgga aacaatcatt ttagttgtaa
     3781 atgcatcacc atacaagttc ttctgcatgt aggtataacc ccagccaggc catgcaaaca
     3841 taccaccctt atagttccat tcgctcttac ctgtataatc ataggcaaat atattctttt
     3901 ccaccatgcg ctggcggctc ttgacaaact cacgatattc attcgttgca tagagattga
     3961 atacggtatc gtcatcataa ccttttacat cgaagaatgg agcaatatta catacggcca
     4021 aataagaatc gttaaggaag gtttcaagtg caaaattgta tggtatttca cggccggcac
     4081 ctccgcacag aggatactgg tcatattcag ggaatttttc agaacgtttt tttaatacct
     4141 ccgtcaggaa tggctctatt tgccgccagt tagtaaattt gactgatccc atatcgagtc
     4201 caagtgcatc tgccatatca gcattgtata taaacgacat gatatagcaa ttgtccttga
     4261 gtgacggaat tccatatatt tgcttgttta ttttcataca ttcccagatc tcattgggaa
     4321 acagagcctt ggtttctgtt ccgtaattat taagcaaatc atcgagcggc cggaaggacc
     4381 cgcggttgga atgcactaca taatccagct tggactggct gccaaaacca atgatgccaa
     4441 catcctggcc agcgctgatc attgtggtca ttttggtttc ccaatccttg gctgaccaat
     4501 agtacatgtt gacattggca ttgatttttt ccttcaaata tttattgacc gcgtcattga
     4561 ccatctgatt gtccggcatc gggtctaaac ccaaatacca atccagcgta acaaaattca
     4621 actctttcat tgttggttca ttaccgccgc tattgtcgcc ttgcgatgcc gaaggcttgg
     4681 ttgaaccaga gcaggcagtg agcgagaaaa tcataaccat aactattaag tacgcaaatg
     4741 tccgggtgat ccataacttt ttcataaata atcctccttt atttggaaat tgtattgcaa
     4801 ccccgatacc gacaagaaaa cagtgaacat caacctttga tactgcccat tgtcaagccc
     4861 tgtacaaaat aacgttgaaa gaagggataa gcaaacagaa taggaagaat tacgatgata
     4921 gtgcaagcca tgcgcatatt ctcgtccgga agtgtacgca gtagtgcgat gccgtctggc
     4981 gtgcctgcga tcgctgcatt ctgtttgagg aaatctatag tgttttgcag cttggtcaga
     5041 agggtctgca gaggaaccaa tttgggattt tgtatgtata gcatgccggt aaaccaatcg
     5101 ttccagcgcg acaccatgtt gaacaggccg actgttgcga tgcctgtctt aaacagcgga
     5161 agcacaatgc tcacgaagat gcggaaatgc ccggcaccat cgatccgcgc ggcttcaaaa
     5221 agtgtatcgg gaattgtggt tcggatgaag gttcgaagaa taaccgtata ataggcgtta
     5281 accagcgatg ggagcaaaaa aatccaaata ctgttattga tgtgcaaata gcgcacattg
     5341 agaatgtaaa tgggcaccaa gccgccgcta aaaagcatcg tgaagaacag cagccaagtg
     5401 aacagacggc tgtgcttaaa ctttttctga gctattacat aagcaaacat ggtcatgaca
     5461 gtcaggctca tcagcgtgcc ggtgatggaa taaaaaatcg taaccaaata agaatctgcc
     5521 agctgatcgc ctgttttgaa caaatacctg taaccatcca gtgtccactc gctggggaag
     5581 aaagtatatc catagtctgc tatgctgatc tcgctggaca acgatactat caggatcaac
     5641 agcatcggaa tcacgaccag caacgccata aaaaataata tgatgacaaa cacggcattc
     5701 cagcctttgg atagcaaggt tagtttgttc actcgtttta cttcttttgc cataaacaga
     5761 catcctttct tggtttacaa tcagaacatg gcactgtccg ggtcgatcct gtttacaatt
     5821 ttgttgacaa ccagtaccaa aacaaatccg accaccgatt gatacagccc ggctgccgtc
     5881 gacataccaa cattattcag gtatgtgaga gaacgataaa tataggtatc aataacatcc
     5941 gtcaccggat agatcttgcc tgaattcatc ggtacattgt agaacagacc aaagtctccg
     6001 cggaagatcg acgacatgga caggattaac gaaatgccta cgatataccg cagatgcggt
     6061 aatgtgatat aacatgcttg ctggaattta gtcgctccat caagaactgc tgcctcataa
     6121 taatcaacag aaattccaga aataacagct agatatagta ctgtttggta ccccacgttc
     6181 ttccatgcat taacaaatac cgacaaagga ggccatatcc taagctgatg ataccaattg
     6241 gtcttaccag gctcccccat taatctcagg atggagttca ccaatccgtt atccctatct
     6301 agaaaggcgt tggccacgat ggcaactacc gcataggaca gaaaataagg catcatgtag
     6361 attgtctgca tggtttttgc gtatccgcct gaacgaagcg agctgagcat cagcgctaaa
     6421 gagatcgaga tgatcagatt aaccacgata aagatcaagt tataacatac agtattgcgc
     6481 gtgatgaccc atgcatcggt tgtgcggaaa agaaattcaa aatttttcaa gcccacataa
     6541 gggcttctaa agataccttt tgtgatattg aggtcttgaa atgccatcac aagcccaccc
     6601 atcggaacat aacaaaaaat aagcaaataa ataagcgtag gcagcgccat caaatacaaa
     6661 gccgcatctt ctttttgaaa tatctttttt cttttcttga gtctgacatt cgctctagtc
     6721 tgaagcatgc ctattcccct ttcatttctc aaataatttg aatatttatc tgtagtgatg
     6781 cgaatccgca gttatataca aactacttat actaggatac tatgcaaact tcaaagaaaa
     6841 tataataatg tcaacttaat gtatgaaaat taatttgtgg caagataata cttaaaatag
     6901 tgatattatc agtgatttat tataagtgat ttaatatcag tgatttaatt gcaaaaatct
     6961 taagactttg actttcccgt attattataa aaaccatcta gtcttcttta ttgtttcgag
     7021 ataccgtttc aactgacatt ataatatatt aaataaaagt aaaagtcaat acgaaaatta
     7081 gcaaaatctg caaacacgag tgtttgacaa ttgaataata aaaaagtcgc tgtaagtctt
     7141 gaaaaaggct tgtttttttg tcaaatttaa aaaattcaca ttgcacaatg cgtcgcaatg
     7201 tgttacaata cctatattat aacaatatga ggtgtatgtc atgagactac aggtttctag
     7261 atccaaaaat gctgcttcct tctatgtaac aaaatctgtc tatgataagg gtaaacgtag
     7321 caacaagatt gtagaaaagc ttggaaccta tgcggagttg aaagccaagc taaacggcag
     7381 agacccctac gaatgggcag aggaatatgt caaagagctg aaccgccttg aaaaagaagg
     7441 acgcgaacct gatgttattg ctaggtattc gccatacaag aggattgaga aggatgtgcg
     7501 ctgttcattc aatggtgggt atcttttttt gcagcagata tatcatgaac ttgaactaca
     7561 taagctttgc aaagatattt cccaaaaata taaattcaaa tttgacttgg attccattct
     7621 ttccagacta gtttatggca ggatactatt ccctttatcg aagcttgcaa cattcgaaga
     7681 atccaaaaag tttattgaac aacccagttt taaattgcat cagatatata gagcacttga
     7741 atacctcgct aaagaaactg attttattca aagtactcta tatgaaaaca gtcttaagat
     7801 atcaagaaga aataccggta tactttacta tgattgcacc aatttctatt tcgaaattga
     7861 agaagcgaaa ggtaaaaagc aatatggagc aggaaaagag cataagccaa accctattac
     7921 tcaaatgggc ctgttcatgg acggagatgg cattcctctc gcattaagta ttaacagggg
     7981 aaatatgaat gaacaactca cattaaagcc cttggaaaag aaaatcctat ctgattttga
     8041 gctttccagg ttcgttgtct gtaccgatgc aggtctttct tccgaagaga atcgaaagtt
     8101 caatgacaag gacggacgtg cctttattac cacacagtcc atcaaaaaat tgaaagaaca
     8161 ccttaaaaaa tggtctcttg cttcaaccgg ctggaaactt ccgggtgacg aaaaaaccta
     8221 tgatatttct aaacttgatg agatgataga aaaagctagt gctaaagata aagaaaaaat
     8281 acgagccaag gttttctaca aagaacgctg gattaaagaa aatagtcttg aacagaaact
     8341 gattgttaca tattctgtcg tatacaggga ttatctgtgt aatatccgga attctcaaat
     8401 agaacgtgct caaaacacaa tagatgacag tcctgaggca ataaagaaat gcaggcaaaa
     8461 cgattacaaa agatttatca gtaagacaaa cataactatc tacggcgaag ttgcagataa
     8521 ggaaatatac ggcattaaca ctgctctaat ccagaaagag gaagcctttg atggttttta
     8581 tggggtttgt acaaatttgg atgatgatgt ttccgaaatc atcaagataa atcaaagaag
     8641 atgggaaatc gaagaatgct ttagaattat taaaagtgaa ttcagggcaa ggcctgtata
     8701 tctgagtgat gacgacagga ttgaagcaca ttttatcact tgctttattt cattagttat
     8761 ttatagactg cttgaaaaga ggctccatga agaattcacc tgtcatgaga ttatcagtga
     8821 acttagagaa atgaatttta aggagattaa gggcgaaggc tatgaaccga tatacataag
     8881 gacagatttt accgatgctt tgcatgaagc ttttggcttc tataccgatt accagattgt
     8941 tacttcaaaa caaatgaaaa aaatttttaa agcaactaaa agcaaatgac attgtgcaca
     9001 ttttggttaa gtctgaaaag cttgaaaccc aacatcatca acgggcttca agcttaattt
     9061 gttctttaag tgtcaaacgg gagagtttga tgcacctcca atcaatcgaa aaatcttgac
     9121 acttacttgg atgggttata taattatttt atcttaataa taactcgaat aaagacgcaa
     9181 tttaccaggg ttactcatct atgatgattt taggttataa taaccataat gaccatcatg
     9241 atgctatatc tgtaagaagc tctcttttca aaaggcgaaa tcctgtgaat aagttgatga
     9301 agcaagagag tggaaggtaa cagacggagg gtttatatat gtacagcctg aaaaatcata
     9361 tagtatacct gtcaagcaaa aggagcgtcc ttattacact gttcacttca ttcattattt
     9421 tgactttaat tcctttaata atttggggct tcttttacaa aaagactgaa tctattatga
     9481 aggaaaatgt aaaccgttat aatatagcta tgcttgagca ggtaaagcat ataatggatg
     9541 ggaacctgca agaaactgag cgtacagcct atcaaatctc attcagttca aagcttcaca
     9601 atattattag cattgattat ccgccagatg tatcaaatac ataccaatat atcgatttca
     9661 aaaatgagct tgctgtttat aaaaggacaa acacttttat ctctgacttt tatgtatatt
     9721 ttccaaaaga cgatgtaata cttatgcctg atatgaagac taattccagt accttttatg
     9781 ataattttta caaatatatg gagattgatt ataattcatg gattgaaatc ttgaatagct
     9841 atcattataa atattatctt cctgtaaagg aaatcctggc ttattcaaaa ccacagaagg
     9901 taataacata tatccaatca ctcccattcg gtaataaaaa ggatattaaa gcatgtctgg
     9961 taatatttat tgatgaaagc aaagtaaaaa aattgcttga taatgtaaag tgggccacca
    10021 atggatatat atgcattatt aacaaagata accaggttat aatgagtact ggcaatattc
    10081 tggataatac agatatacct tactccgagt ttaataatga tgtgagtgta tataaagaca
    10141 aaagcggttc tgaaattatg gtgtattgtt ccatttcaca ggaatcagac tggaaatata
    10201 ttagtatttt gcctaaaaat gaatttatgg aaaaggttaa taatatgaag cgttggattc
    10261 aaaataacat ctttttttat ttagtattgg gacttatgct ggcattcttc cttacatata
    10321 agaattataa tcctgtaaaa aacctgataa gcgcaatctt tagcgaaaag aaaattaagg
    10381 ataaaaatat tcagaatgag tatgacttta ttaagcaatc tattctggat ggtatgagcg
    10441 agaataggaa attaagcgat ataatatacc gtcagatgcc tgctgttaaa agcaattttc
    10501 ttatgagagt catgtatggg ataattgatt attctacaat cacaaaggaa tcgcttaaat
    10561 tcattgatat tgattttgta tcagattatt ttatgctttt aataattgaa atagagaatt
    10621 tcggaagcat tttttcgcaa gattctgaaa atgaaaaggc actggcaaga tttgcattaa
    10681 caaacatggg aaatgagctt gtcaatgaat atggtaaggg ttatctcatc gagcttgaga
    10741 gcagcagact tgctctttta tttaatgttt ttgaggaaag actggataaa gccgaatctg
    10801 atttgttaaa catatccaaa gtgctcagga acgcagcaca ggaacatttt gatgttttta
    10861 ttacaatagg cataagcagt attcacaaag gtacttttgg aattggtgaa tcatataaag
    10921 aagcttcaag agctattgat tacaaatttg tgaaaggtat tggtgaaatt atttgtttta
    10981 atgaaatcga gagtgtgaaa caaaattatt attatccgct tgagtcagag ttgattatta
    11041 ttaatactgt taagaacggg gactacaaca gtgtggaaaa gatactggat aaaatatata
    11101 acgataactt caaaccgtat aatgttgcgc ctgaacatgg aaagagcctt tttttcaata
    11161 taatgagtac attggttagg ataatgaata ctactaatat caagttcgaa aatattttaa
    11221 ctggcgaaac cgatatagtc aattacttga caggatgctc tacaattgaa gaaatgcatg
    11281 aaaaaattaa aaaggctttt aaattgattt gcctttatat tgattccaac tggaataaca
    11341 gaaaaaacca tcttctagag caaatcaaaa actatattgc gaagaattat actgacaata
    11401 ccttatgttt ggtatcaatt gcagataacc ttaatataac acctcagtat ttatctacat
    11461 attttaagaa tgccaatggt gagaatttaa gtgattatat agctaaaata agggtaggaa
    11521 aagcaaagca gcttctggca gaagggcagc tcacagtttt tgaaatagct aaatcaattg
    11581 gatatacaaa tgatgtggga tttataagag tattcaagaa aattgaagga gtaacaccag
    11641 gtaaatataa gtccatgatt tcatctgagc ataagtgata tgctatataa agccatttac
    11701 cttggacagg aattttatct ttatatttga taatcaatgt ttaaaattgt aaacccaatt
    11761 aattaacttt atatagtctt tattaagtat gtatagttta cttttgaagc atttccggta
    11821 gaatgaatat gcagatacag aaatgccgcg gatgtttctg attgatccct taatattaat
    11881 tattaaaagc agaagttaat aattgaaagg taaagttaac aattaaaagc agaagttaat
    11941 aattgaaagg tgaagttaat aattgaaagg agaaagatat gagaaataat attatacggc
    12001 taatagcact ttttgtttct gttttcttag tgatgctgct atttagcgga tgcggacaaa
    12061 cgtcgaaaaa tgggaatact tccaatcaga caactgcaag cagtgctgca gactcaataa
    12121 caggacagga aaagaacaac acacttatag tagacaaacc tctaaccctt acttattatg
    12181 tagctaattc agcgagagga caggtacagg attataatga aatactggcc tataaggaaa
    12241 tggagaaaaa gaccggcatc catatcgaat ttcaacaccc tgttgctaat acacttgagg
    12301 aaagagtaga acagttcaat cttatgctgg catcaggcga atatcctgat ataataagtg
    12361 ttgcgtggtc tgagatgaag caaactccag agcaattgat agatgacaat gttataatag
    12421 gtctaaatga attaattgat caatatgctc ctaattataa aaaaattctt gatagtaatt
    12481 ctgatataag aagaggagca gttactgata aaggcaggca ttttgcattt aagaatgtat
    12541 acaatcatga atctacagct gtatggttcg gtcctataat caggaaggat tggcttgaca
    12601 atctcagtat ggatgtacct gttacaatag atgaatggta tacagtactg caagctttta
    12661 agagtaagga tcctaatgga aacaacaagg ctgatgagat acctcttttg attggggctt
    12721 tcggtgatcc acttggttca tttagattag gtcgcgcctt tataggagct tgggggattg
    12781 caatggaata ttatcaggat aatggaacag ttaagtatgg tcctttacag cctgaattta
    12841 aggaattcct gaaagtgatg aacaaatggt atattgatgg ccttattgac cgtgattatg
    12901 ctgcaatgaa aacagagctt cttgtagcca atgtgacaaa caatcttctg ggttctttga
    12961 ctgcaggtgc ttctggagct ataggcagat acacaaatat gatgaaggat aaggattcga
    13021 aattcaaatt ggtaggtact ccatatccgg tactgaaagc tggagataag cctcagcttg
    13081 ggcaaagaga tagtatggtt ttaaatatcg gagctgcaat aacaaccaaa tgcaaaaata
    13141 agattgaagc tgtcaaatgg ctggactatg cctatagcga agacggcaat atgcttatga
    13201 atttcggagt agaaggtact acatacacta tggtagacgg gtaccctaaa tatacggatt
    13261 tgattctaaa gcccagggat atatcagtag atgagaatca ggttaaatac tgtcctacca
    13321 atatgggggg accatataca ctggattacc gttcatatga acaacttatc agtctgcctg
    13381 agcagaagga cgcaattaag atatgggccg ctactgatac atctaaactg atccctgaca
    13441 atgtagttat ctctccggaa gatttagcaa aaaatgctac gagggaaaca gatattaaaa
    13501 cttatatgga tgaaatgatt aataaattta tcatgggtgt ggaacctatt gaaaactatg
    13561 ataagtttgt agaaaagata aaaacattag agattaattc gcttattgca gcaaagcaga
    13621 aagctcttga taattacaat aaacgttaga atcttaataa ttacaataaa tgttggaatc
    13681 ttaataatta taataaatgt taaaatctca ataattataa taacataaaa ctactaaaaa
    13741 tgatctgtgc agaaagatca ggagtttgcc cggaaagtct gagctataaa gcggactgca
    13801 tgagccagct tcttcgagca gactcctaat gaatatcttc cctgtacaga tacttctgtc
    13861 aaaggatttg aggttaatga acacaacaaa tattaagcag acccgggcga agcagacccg
    13921 gacggagttt gaatccggac tacttaaaac aaaacttaaa acaaaactta aaatattata
    13981 tgatgattta aaaagaaata agcttatcta tttaatgctt attcctgctc ttgtgtatat
    14041 aattgttttc cgttatgtgc ctatgtatgg cgttcagata gcatttaagg attatagttt
    14101 ctccaggggc atatggggaa gcgaatggat tggacttagg aattttagag atttttttac
    14161 cagcatttat tgctggcggg taattcgcaa tacaatttta attaatttga tatcactgtt
    14221 ttttgccttt cctattccta tcattcttgc actgctttta aatgaagtta aatgtatggc
    14281 ctttaaaagg actgtccaaa caattacata tatgccgtat tttgtatcac tcgtagttac
    14341 agttggcata atatatgatt ttttctccaa caatggtatt gtgaacagca tgctttcata
    14401 tctgggaatt gaacctatac aattcctgca gaaaccccaa tggttcagac ctctctatat
    14461 aggctccggc atatggcagg aagcaggctg gaattcaata ttgtatcttg cagcattaac
    14521 caatattgac ccaaccctat atgaggctag tgaccttgat ggtgctaaca gatggaaaca
    14581 gacactgcat gtaacactcc cgggtatcat gcccgtgatt gttattatgt tgattttaag
    14641 aatgggtaat atgatgaatg taggcattga aaaaataata ctaatgtaca atcctttaac
    14701 actggaaaca ggggaagtca tatctacata tgtttacaag aggggtatcc aggaaatgtc
    14761 atacggtttt agtgcagctg tgggattaat taactctaca atcaacttga ttctgctgac
    14821 ggcatcaaac tggatgagca gtaaactgag tgaaaacaaa ttatggtaag gagtattatt
    14881 tatagtaagg agtaattagt aatgaagaga agaacatcag gcagtataat ctttaatgta
    14941 tttaactatc tgtttcttgc attaatcagt atgatagcac tttatccgct ggtttttata
    15001 ttattcgggt ctatcagtga acctgtcctt attgcgcagc acagagggct gttgttgaaa
    15061 ccacttggtt tcagcatgca ggcatataaa attgtttttg acaaccctat ggtagctctt
    15121 ggctacagaa atacacttat ttatcttgta ttaggtacaa caataaatgt tgctctcact
    15181 tctcttgcag catatgcact ttcaaggaga aatatcctgc ttggcaactt cataatgttt
    15241 ataattgtgt ttacaatgtt ttttgatggt ggtcttatac caacttatct gcttataaaa
    15301 aggctaaata tatataacac aatatgggca gttattattc caaatgcaat gagagtatgg
    15361 tgcttcatta tcatgagaac cggctttcaa caggtgcctg ctgaaatgga agaatcagca
    15421 aagctggacg gtgctaatga tctgcagata ttatataaaa taattattcc tatttcaatg
    15481 ccggtggtcg ctgtaatgat cctgttttat ggggttgacc attggaatag ttactttaat
    15541 gcaatgattt ttcttcggga tagaaaactg ttcaaccttc agcttgttct aagagagata
    15601 cttattgtaa acgatacaac aaagacaact gcccggtttg caagcgaaga tagatatgca
    15661 gtacaaattt caataaagta tgctcttatt attgtagcaa ctgtacctat attactggta
    15721 tatccctttt tacaaaagta tttcactaaa ggtattatgg ttggggcact taaacagtag
    15781 taggaaacag aagtaaaggg aaggaaacat agggacggta tttagtaaaa ttttgttgaa
    15841 aagttaacaa aaaactatta ccaattatac tactatgatg caaaatgaat gtataggtag
    15901 tattaattta catagggggt tggatatata tgaagggaac aggcattgta aggaaagtag
    15961 atgaattagg aagaatagtt attccaaagt aattaagaaa agtattagat attaaggtta
    16021 aggatccaat gaaaattttt actgaaggtt catccattat attaaagaaa tatagtccaa
    16081 ggtgtatctt ttgtgaaagg gtaaataatg atattaagtt taaataaaat aatatatgtc
    16141 ctgattgcct gggaaaaatg aaaaatgacg attagtatgc aagataccga agaattttgg
    16201 taatcaagtt ctgctgtatc cctgctgttc agcttttgaa gaagccagaa gacagaaacc
    16261 gtattgttcc atggcactta attggtgcta taacgagcca tggccatgtg tagcaaacaa
    16321 tagtctgctt tcttatcctg cagcacctaa aaaagcttac catgcagcag ctcaatcttg
    16381 ccggcctgtg ctgctgagtg cccgcttaaa ccgtttttcg tataaggcag atgatttgtt
    16441 tgaggctgtt atatggctat tgaatgactc acctgttgat attgagcctg gaaaagcaat
    16501 tgttacccta acactaggta aaaattcata taatttaact gaatgggaat atggaaaggc
    16561 gaaagcaggt caaaatatag aggggccaag agtaagcatg cagctgcctc ttgcggcagc
    16621 agactgccta atattaacca taaatgccgg tgaatattca agtgagtata gattattata
    16681 taggctcggt gcctgagact tgttttttat atttaggttt tgtaggaaca tggggacagt
    16741 tcggtcctca tgttccgttt tagtgaaata atacacagtt acagtgaaat actgcaccaa
    16801 ataatgcaca gtatttggta caatcacagt gatactatta cggtaatata atcacagcgt
    16861 tataatagta agactacaga tgctgacgtt ctttcgtcat aagcttgact ttgattttta
    16921 taatgtattt gtatatgttt ttaccattaa tctttcttta tttatatata gcaatgacct
    16981 agatgtaata tgcaacatat aatatgcaac atgtaatatg caatatataa tatgtaatat
    17041 gtaatatgta gtatgcatta tagatagtat ctactggagg attttatatg tttaataaaa
    17101 ctgataaatt acatgatact aataaattac ctatgactga tataatagat acaactgata
    17161 aaagccacag aacatccagt attacccaga atttatatat gaaagctaaa aaaataatac
    17221 cagggggaac acagctatta agcaaaaggc cggaaatgat ggctcctggg caatggcctg
    17281 cttatttcag taaagctaaa ggttgtgaag tatgggattt agatggaaag cactactatg
    17341 atatggcgac aaatggtata ggtgcttgta ttctaggctt tgccgaccct gatgtgaatg
    17401 aggcagtaag gtgcagaatt gacatgggga gtatgtgtac tttgaatcca ccggaagagg
    17461 tggaacttgc agaattgctt tgtgatatac acccatgggc ggaaaatgta agatttacaa
    17521 gaacaggagg agagaccgga gctgtagcgg ttaggatagc gagagcgaca actgatcgat
    17581 cagtggttgc aatttgcggc tatagtggat ggcaggattg gtatctggca gcaaatcttg
    17641 gagaaagtga tgctttgaga ggacatttgc tccctggact ggatcctgta ggtgtaccca
    17701 gggaattaag aaatacaact gttacattca gatacaacaa caaggaagaa ttcaagtcgg
    17761 ttattgataa atatggcaac aggttggcag ctgtggtaat ggagcctgtg aggtacaatg
    17821 atcctgaacc gggctttctt gaatatgtaa gagatggaac ccataagtgc ggggcattat
    17881 tggtatttga tgagattaca ataggatggc gcttaatcta tggaggctca cacctgaagt
    17941 taggtattaa tccggatatt gccttctttg caaaatctct tggaaatgga tatccaatgg
    18001 gagcggtaat aggcaccagg gaagcaatga aaggggcaca tctctctttt atcagcagta
    18061 cttactggac cgaaagtata ggacctgtgg ctgcactagc tgctttaaaa aagatgaaag
    18121 aagttaaagc ttatgagcat gtctccaaaa ttggtacact tgttatgaat ctatgggaaa
    18181 ttaacgctca gaaacatggt gttgaaattg atgtggggaa aggttatcct tgccttgcac
    18241 attttacttt taaaaatgat ttcgacaata aattaaagac actttatacg caattaatgc
    18301 ttgaaagagg attcctggct cctacttcca tttatccaac tgttgctcat actgaagaga
    18361 ttgtaaaaaa atatggagat gcaattgatg aagtttttta cttgattgct gaagcaaaaa
    18421 gaaatgaaaa agtagataat ttattaaaag gccccgttgc tcatgtggga tttgccagat
    18481 taatttgagg cttattagtt cataaacaag ttgaaatata gtttcaaata gagactttaa
    18541 ctgtgataat ataacaggtg catatttctt cacttttaga aaatgcatcc taatataacc
    18601 ttatagttga ttgaaatata aatataggct attattgaat tatattttcg ttttaggtat
    18661 taagtttcag atattaagtt taggtattaa gtttcagata ttaagtttag gtattaagtt
    18721 tcagatatta agtttaggta ttaagtgtag atattaagtt ttaggtttta agtcttaagt
    18781 tttaaagtaa atttttgcag taagaagaat cccgcgataa tatattttta gaaaagttat
    18841 cgatgaatag ggggggtgtc ggcttgtaca gacttatgct cgtggaagat gaggaaatta
    18901 tctgtcatgg tttaaaaaat aagataccat ggaaagaggt tggtttcgaa gttgtgggcg
    18961 aggcaaatga tggagaaact gccttgagga tgatacctga gctatggccg gacgctattc
    19021 ttactgatat acgtatgccg ggactggatg gtatcggctt tatagaaaag ctgcgggaat
    19081 tgggttcgaa agccgaggtc gttgtattaa gcggatttga tgactttgaa tatgccagga
    19141 aatgcattgc atttggcgta acggaatatt tactcaagcc tattaaaaat gatgtgattc
    19201 taagtacttt ttatcaattg aaatgtaaac tcgatagtcg atacagcaag gattctgaat
    19261 ttaataatat taagaaaaaa atcgagctca caatcccggt tgcaaaggga aaaattattc
    19321 aggatatttt agaaaaccgc ttgaacaaag aggatacgga cagaatacat gctattttgg
    19381 atatgccatt tgataaaact gcatcttttg gcgttgctgt gttccatatc agtcgactga
    19441 tgaacactaa aataacattt gatgatggca gcagagattg ctttcaacga gtgttggaga
    19501 atgagacagc acgtttacct caataccaac tgctgagcgt gttattccaa aaggacacat
    19561 acacaatagt tgctgtctgg agcgtacata gaaggcagaa agaggataca ggacgtgatt
    19621 tgtgtaatgc aataagagaa aaattagaaa atttacctgc ctatactggt aaaatccttg
    19681 tcagcgtggg agttggatca atttttgata atatttttgg tttgagccgt tcctatatac
    19741 aagccaagaa tgcgcttatg caaaaatttt acattggtct cggaaaagta atcagttaca
    19801 ccgaaattca gaacgaacct ggacgcacta aagtgcaact gttggacaaa accattgata
    19861 gcaaccaatt agtgagaaaa atcatagaat atattgtttc aggagatatg atatcctccg
    19921 aaaaatcagt taaatgctat tgtgatactt atttgggtat gcgcagatat aaccctgaaa
    19981 ttttattcat gcgtgtaatt gaactcgtgc taaagctggc gggtgccttg gaagaacagg
    20041 gtatttcctt tacggatgta tatggtgaag gtgttgatca ggaaatcaga tttttagtgg
    20101 aagaaaaaac cattgatgaa ctgcgggaat ggttgattat gctggctaat cgattaatca
    20161 agcagtcaga gagaacagaa gatacatatt ccattgatca catgattgag gttgtcaaac
    20221 agtatgtcca aaaaaattac aatcgtaaga ttaccagcaa agagttatgt gatatagcta
    20281 tggtgagtcc ttcctatttt tcaagcatgt tcaagcaaaa aaccggttat acgctgatgg
    20341 aatatgttat catggtacgg atccaaaaag cgaaggaact gctggccaag tctgactaca
    20401 agatctatga ggtcgctaca gtagtaggtt atgatgattt tcgccatttt agcaaacagt
    20461 tcaaaaagct cgaacacatc agtccgagca aatttaggaa ggataacgag gaaaaactat
    20521 taaatcgcaa taggctatag gcaataggca ataggcaata ggctataggc aataggcaat
    20581 aggcaatagg taataggcaa taggtaatag gaactaagta atataataag taataaggga
    20641 agatacattt atgaaaaaac ccaaaaggaa aaatactatg tctgtgcggc tgtttcttaa
    20701 tctaacagct gccttgctgt gttttctctt tctcgtaatt gagggtataa tttcctttgg
    20761 tgtatataaa aatattgtcg atttgaaagt tggggaaatt taccagaacc aactacagca
    20821 agtgactgat aggatttcat ttgaattacg tctaatcgaa aatcttatta atggtatagt
    20881 ccaaaatagt gaacaaaatc gattttgctg ccgatatcag gaagaaaaaa atatgtatac
    20941 taaaaatcag ctgcaaaaag aaattaaaaa tgacctgaat gagcgaacta ttggcttaaa
    21001 agtcagtgga tttgagattt ttttagaaga taacagtact atgatgcaaa ataagcaaat
    21061 aatgcttgca aatttcgcac cagaatataa aagtttacat tcgaagctgt cacagtctga
    21121 gcgtaatgta gttctgattc caaccctgga tgatgagagt ccatggattg accggaataa
    21181 tcatattatg tttgcagctg cactaacaga aggtggagaa gaagcaggta tattggtttt
    21241 gcaaatggac agcacatgga tagatagcat gctgggtgtg ggaagtaatg ccatgctgtt
    21301 tgaacgggag aatcaagtta tcctgggtgg ttttatgtat gaaaatactt ttcaggcaaa
    21361 gccaattttt gagaaaatag atggagagaa cggaagtttt acggcacata cagaacaggg
    21421 ggattggaag atccagtatc aaaatataga aggaagcagc ctgaccgctg taattcctaa
    21481 aagtatatca gagctattta gtgcaattaa aaatgtacta aagctagtta tagtgttcac
    21541 actccttagc agtgttgctg gttttgccct tggtgaacta ctggttggaa agactgtcag
    21601 gcctattcat acccttctgc atgaggtcaa aagtcttgga gctgagagcg cggggccagg
    21661 aaaagaggag cagcgtaata tcaacagttc aatgagaaat acggtgtttc tttattataa
    21721 tgtggttatt ctaattccta tgctggttta tattatgaca ttctattttg gcgcatctaa
    21781 taatattgag catattgtta aggattcgct gactcaggta gtggaaaaaa tagtacatga
    21841 tatagcagta tatatggaag tcaatgagag tgcatctatt aagattgtca tggatagcac
    21901 cgtacaacat tttttaactg aacatgaaga gaaaatgagt gaaattactc tttcagatat
    21961 tgtgaagctg gacaaagtag taaaaaaaag tatgactttt gcaggtacaa ccacaaatgt
    22021 tgaaattttt gatagtaaag gcattcgagt atatgccggc aatgattatg agactggtag
    22081 taaaattagt gctaattata aggatatggc agtcaagtcc cggggtgacg cagtatggaa
    22141 ttataccgac atagatcggt atggaaaacg gatgatctgc ctgatacgca gaatttatga
    22201 tttgtgtatg gaattgcccc catttcgcac aataggttac atcaagctga ctatacctga
    22261 gactgaaatt aaaaatcttt attataatat taaatatgat aacagtgact tttttgtatt
    22321 ggataaaaaa ggattgtatg tatccggtcg caataccgct cgtattggag aaccatccgg
    22381 gtatatgggc tatataggtg atggcatttt cgcggggagc cgaaatagaa aggttgtcag
    22441 aaacaataaa ctctatgata tcataactgc ggaagacagc cgcatgccac tggcgtttgt
    22501 tggcgaaatt tcgcgtgatg atattataaa aaataataac ctgattattt catcaagcct
    22561 gtatcttatc ctgaccatat gtatagtgat atttattctt tcctactcta tttcgcagtt
    22621 ttttgcgaat gcattcaata atatgactca taaactgata cgctttttta aaggtgactt
    22681 gcaagtggat ttttcatctc atttctattt tagcgagatt ggtcaattag ctgatacttt
    22741 taaccatatg tccgggcgaa tcaacgacct gacagaaacc ttgtataacg tacatttgaa
    22801 agcaaaggaa ctggaaaaag agaaaaaaga atctgaactc atcgcacttc aagcacagat
    22861 aaatccacat tttttatata acacaattga atcaataaaa tggatgatga aatataacaa
    22921 tgaaagtgct gagaccatgc tgaccaagct aggagagctt ttccgcctgg gaataagcag
    22981 caaggtacag cttgtaaccg ttgcagaaga aattcgttac gctaacacat atattgacat
    23041 acaacgactg cgttataagg atcaggtaaa aattatctgg gaaattgatg aaaatgttta
    23101 tgaaaacact atgcccaagt ttacccttca accaatcatt gaaaacgtgt tcaagcatgc
    23161 attccgtttt aaaaaagaga actgtatcat acacatatgc ggttacttag aggagaatag
    23221 tgttgttttc agtatcatgg ataatggaga tggtatccct caggatcgcc tttatgagat
    23281 taatgagctc ttgcaaagtg accttccggg agagagtatg ggtatataca atgtgcaaaa
    23341 gcgaatccga atctattatg gagatcaggg cggaattaac tttattagtc aggaaggggt
    23401 tagcactata gtcaaggtgt gcatacctgt agaaaaaaac aatgataata atatacaaaa
    23461 tatgcaaaat agtacacagt tacagtgaaa tattgcacca aataatgtac ggaatttggt
    23521 acaatgatag tgttataaca atgaaactca ggcatgccga tgttctttca ccgaaacttt
    23581 taaagctaaa aaattaaaag ggaggatata ttatgaagtc atcttataaa cttgtctcta
    23641 tcctattaac tctgatgctg tgtgctacaa tgttcgcagg ctgtacattc accacaaccc
    23701 aaaaggatga taaaactgcc acagaagggg aaaacaaagc aactgttacc gttaaaccta
    23761 aagtggatgt cagccttgaa ttggttttcc tgcctactac cctggcagaa gaccaaagca
    23821 gtaatgttat agctaaatac attgaggaca aattcaatgt tagtttgaag tttaaaccat
    23881 ccgatgcaga aaaacaaagg ctccacctca gtaccggtca ggctgcagat attatttcgc
    23941 tggcaccatg gctagggaca accgacctga ttaccaaggg cgtcgaagag gaaatttttg
    24001 tagacatagg caaattggtc aatgcagagc ccggaagata tcctacattg gcaaaaatgt
    24061 ttaaagagcc gttgtatcag tattttaatg gttactatta tggtaagccg aaagaatttt
    24121 attccatgta tgctcttgat tataccagaa atgtagctgg cgcaccgatt tacaacaaaa
    24181 aaattatgga tgagcttggc ttgaaaatcc caaaaacagt agatgagttt gttgaatatt
    24241 taagaacagt gaaggcgaaa aagccaaaca taataccttt tttcttccgg aatgacaagg
    24301 gttcctggtt gaccggaagc tccggagaaa taaatcagat ttttttccag acttatggtc
    24361 tgaatgcatc cagcgttcaa aaagataagg acggaaaatg gcgtgatact tccattgatg
    24421 aggcaaacaa agtagtttgg aagcgcctgg ccggcatgta tgctgagggc cttattgacc
    24481 aggaagtttt tacaaaagaa gcagatactt ttatggccaa cgattttgtt accggtaaga
    24541 tcgctgtagc agtgtctcat ttgcctaatg gcaatcctaa ctcagcatta tacccttatg
    24601 atgaatttaa aaagggcaat ccggatattg atccggctac attccttgat tatgtagtct
    24661 atcagcctga accgctcaca ggaccggcag gtcatggaaa gaccagagct gttgccaaga
    24721 gtgttccatt ttgtagtttt atcaccagta cctgcaaaaa tcccgataga gctcttgata
    24781 tactcaactg gattatttct gatgaaggcc agactgttct gaattatgga attgaaggcg
    24841 ttcactataa aagccttgat gctaaaggaa gagtggttgg ctataatctt gagaagttca
    24901 aggaagacaa catggggtgg ggatttggtg gcttaacctt cggattcctt cttctttcag
    24961 gcagcggtac ccggtatctt cccgaccgtt atggctggag cgaatcggtt atgcataatg
    25021 aaaatctcgc tgaaggcggt ttttcaactg agcttccgaa aaatcttgcc gattatggta
    25081 cagaaatcta caataagtgg tcgactcaat gttatgagga acttccaacg tattacaact
    25141 atataagttt tacagatgag ttcaaacaga taggaaaaaa agtcaatgaa atcaaggtta
    25201 aatattttgt cggctttata ttcggaacat atgatgttga taaagattgg aacaaatttg
    25261 tgcaggaatt tgaggatgcg ggaggaagca aatatgccgc agagtatgga cgtttgctgg
    25321 atattgaaaa agaaaaattt gaggctttgg gcaagtagtt atatagttat atagttatac
    25381 agttttgcaa ttatacagtt atgcattagg ggagctttga gccccctaat gcataactac
    25441 ctaccaattt aactaaagca tcaggaagtt tttcgcttct acgcttctat gcttctatat
    25501 gcgggagctg aattgatgca aattataact acaaacttgt tcagtggggg cttacaagac
    25561 tcctactgaa ttcccggact aagcagtttg ctattaggaa agtgagggcg gataatggag
    25621 gttacgctaa aaaaacagga tcgtgagagc tttattgaac gattgaaata caacataaaa
    25681 atgtattggt ttatttatct gttggtgctg ccaggtataa ttcatttgat aatttttcgc
    25741 atttccccca tttatgggat ttctgttgct ttcaaggatt acaaaatcag taaaggcatt
    25801 ttgaagagtc cctgggtggg attcgacaac ttcaaaatac tatttacaga caaatatttt
    25861 tacaaagtaa tctggaatac agttttgctg aacatttatt caatcatttt tggaacggct
    25921 tttacaatat ttcttgcact gatgcttaac gagattaaga accaattttt gaaaaaaacc
    25981 ctgcagacaa gtgtatattt tccaaatttt gtttcatggg taatttttgc aggaattgta
    26041 aatgctatgt tatcaccagc agatggagca gtcaatttga ttttaaagct tctagggtta
    26101 aaatcaattt attttcttgg aagcaataaa tggttcaggt ttattattgt agcttcaggt
    26161 attattaaaa cagcaggatt tggtacaatt atttatcttg cctccatcgc aggtataaat
    26221 cctgaaatat atgagagcgc ttcaattgat ggagctcacc ggggacagct tttatggcat
    26281 attactctgc cgcgcatcaa gcctactatt gcagttatgc tcataatgtc agtatcaaat
    26341 atcttcggtt caaactttga acaaatctat aatttgtaca gtccgttagt ttatgaaacc
    26401 ggcgatgtgc tttcaacata catgtacaga accggtctgc tggaaggtaa atttgagaca
    26461 tcaactgcgc tgggattggc ttttagcgta atcggtctcg gtattgtcat tatgactaat
    26521 aaagttgtaa ataaaatgga tgttatggga ttcttttaag acatagcgag gtttacataa
    26581 gtttagccaa aaattgtcag gccaagggtt gactagacac tatgcagctt acagttcaca
    26641 acttgctata tatatatatt acaattttta tttgctgaaa ggagtcaatg ttatgccatg
    26701 aatatgaaac ataagctggg atttaatttt tatgatgtgt ttgtatatgc ttttaccatt
    26761 gcctttgcta tattttgcat ttacccgatg tggtatgtat ttatcggttc cattacgcca
    26821 tctgcatcaa gtgcacagca gccgctgcgg attttgccgc cacacaatcc tacattagaa
    26881 tactataata ttgtgctgca tgccaaggag ttcaagcact ccatgtttat ttcattttta
    26941 aagacaatag ttggcgcgat aggttcattg atttttactg ctatgatggc ttatggtgta
    27001 tccaagcggc aggtcatggg tatgaaaact cttaacttta tcattgtgtt ttttatgttt
    27061 ttcagtggcg gactgattcc gacttatctg ctatacggac ggatgcacct gcttaaaact
    27121 ttttgggtaa tggtaatacc gggatttatc agtattggcc actttgttat catgcgcaat
    27181 tatttctgct ataacgtgcc ggaagatctt gaatctgcgg ctgttatcga cggtgcgaat
    27241 aatattataa ttttctttaa aatcattata cctatttcca tgccaatgtt tgcagctatt
    27301 tttctattcg aggctgtagg tcattggaat gactggacga gttacttgta ttttgtagga
    27361 gaggttaaat atatgccgtt catcgtagtt ttgcaggaaa ttctacgtaa tcccaatggt
    27421 cagattatga gggatgctac tgctactgta atgaatatac agcagcgtac ccttcctcct
    27481 gcttcgctga tcaacactac aattattgtt gcgatgctgc cgattttggt aatctacccg
    27541 tttttgcaga agcactttgc aaagggtatt ttaattggtg cagtgaaggg ctgatagttc
    27601 taccagtata gtgaaaagct gatagtttta ccgtaaatat tttgaataaa gtgagaggta
    27661 agctaagtgg gagataagct aatagatatg aacaagaagc aacctaaaaa ggcatcagtt
    27721 gtaatcagat caaatagtga gaatgtgata cgttttgcag cagaagagct gtgcggctat
    27781 ctagaaaaac tgttcgacgt agatgcttca atttcggaag agtcgccgga aaatgcagaa
    27841 atattgtttt atgttggtac acaacaagaa aacccttatt ttttggaaaa tggcatttca
    27901 gataaacttg aagcttcaag caagcaagta gtaatgagca agcaagtagt aatgagcgaa
    27961 caagtagtaa tgagccaaca agtagtaatg attcggcgtg tctcagaaaa tagcatggta
    28021 ctttccggtg gaagtcctgt agcggtactt tgggctgtgt atgggttagc cgcacagtgg
    28081 ggtgtagaat ttttgctcca caatgatgta ctgccagaaa agtgcactct ctatttgccg
    28141 gatgtagata tatgcgagga accgcttgag ccggttcgga cctgggcatt tccccatgga
    28201 cttgcatttg gaccggaaag ctggggaatt tctgattatg aaaatatact catccagctt
    28261 gcaaagctga gatttaatcg aatatacatg caattttacc cggagtttcc acttatagac
    28321 ttggaaattg gcagtatccg ccgctcaaca accggactat tttttaacta tcattacccg
    28381 attacagatg acatgattgg tcgggatttg tttgatgatc gcagtgagtt ttggaaccgt
    28441 gacctgcctg ttgaagcaaa ctgtgcagaa atgcttgctg cagggatgaa actggtacac
    28501 ggtgtaattt ccattgctca caagtttggt atggaattca taatgggtgt actgcctctg
    28561 gaatatacaa gggagtttgc tccaatatta aaggatccgt tatatgttaa actgttaaat
    28621 actgagacag tcgtacccgg agcaagtatc aaagtagagg atgaccaact ttttgagatg
    28681 gcgcttgcag tcgttcgatc tgtggttgac aactatccgg acattgatat aattgcacct
    28741 gttatgccgg aattccgcga ctgggtggaa tattcggaaa cagcatggca gctgctcaat
    28801 gaaaaatatg atattgagga aatatgtacc tataatgagg ttatggaaaa ggcggcaaga
    28861 cgtacggaat accctggtgg aggtgcaagg gcagttaaag aggtcaaagg tgatatagtt
    28921 gcattgtatt tctacgattt gtttttccgt gaaaaaaggg caatgagtta tacctgcaaa
    28981 ccggatatga aattgatgta tatgtatctt gctgaggaat tatttccact gctaacccgt
    29041 attctgccgg aaggtacaga aattacgtgt ttcgtcgact acacaccaac gaggatactt
    29101 aaaagatgcg aagcactcgg tacaattcaa cctgaaacta tctatacttt gtgctataca
    29161 ctacacgatg ataacattgg ttttctgcca atggtcgcag cagaaagttt gggtaaattg
    29221 cttacaacgg cgcgaaaggc tggttggaaa ggttttatga gtcggtattg gttgaccagt
    29281 gaccacgata tttgcattaa ttatctggca aacgcaatgt ggaatagcag ccagagttta
    29341 caggcagcct attggcgttt aatatctgcc atgtgcggca atgactgtgt atatgagttg
    29401 agtaatatgt atagtgagct ggaaaatgca acagtaattc tagaaagaga aggattgggc
    29461 tttgcattcc ctgtgccgga tatggtgagc tatcattggg ggaaaaagga accatccaag
    29521 aatatggacc aggtgcgcct ggattatgaa caggcacttg actgggcaca aaaggctcgt
    29581 cctaaagctg ccgaaaaggg gatgtggttt gtcgattact ggattaatcg gattatgttt
    29641 ggaatcaaat acctgcaaat gacggaggca gtattcgagg gaggacaggt tgcagcaaaa
    29701 gggtatataa cagaagcaaa agcatatgta aaacatgcgt tggatcttgc tgaggaagcc
    29761 tgcattagtt atgccaaagc agcaagagac cgttctgatg ttgcttcaat tgcaattttt
    29821 aacgaatatg gcatcagata tctcaagaag aagctcgaag atttgaattc acaaacgaag
    29881 ctataattaa tataggaata tactgtaaaa taatataaat atttgtaagt agtatatata
    29941 attgcatatt catggaggaa ttattatgaa caatattatt ctttctgcac cactcaccca
    30001 cagtgactgg tacttgaaaa ccaacggacc tgaatggggt actgaaggta tacgcactat
    30061 gcttacccgc tgtaaggaat ttggattttc aagaatatat tggcgcgttt atgattctgg
    30121 taaggcgacc tatgcaagca aactggttga gccatttcgt gctgacgagc atgaagaaat
    30181 ttggcaatat tcaccggggt atattgatct tccaccggaa gaaacaatga agcgttttat
    30241 gcagcttgac tatacaaatt ttgattcgct tagtgcagca attgaaatcg gacatgaaat
    30301 cggactcgaa attttcgcat ggatgtccat caacgaagat gaccatggca ttggttttac
    30361 cagtaaattt accagagaga atccccaata tcgctggata tgccgggatg gaagaagcta
    30421 tcattctcag ctgagctatg cgttttcaga ggtgagggaa tacaaattag ctcttattcg
    30481 cgagatgctg gcatatgata ttgacggctt gtttattgat tggattcgta ctggtgacat
    30541 tcgggataac cctcagtcgg atgtggacgg agtagcagat tatggttatg agaaacctaa
    30601 cattgaagcc tttatacaag agtttggtat cgatccacgc tgtgttccga ataatgatga
    30661 aagatggatt cactgtcgtg caaaaccaat tactgaattt atgcgtcaag ctcgtgcatt
    30721 gaccaaaagc cattcaaaaa agctgcgtct aattacgatg gtacatcacc catggtctta
    30781 ccgtggtgta atgcctgaaa tgataaacga agacacacct caatgggtca gaaacatgag
    30841 cggtaacagg gttgatggcg ctcttaatgg cctattatgt gatattaaaa cctggtcaaa
    30901 agaaaatcta atggatgatg tgattgcagc tggttactat ataaaaggag gaaatgcgtt
    30961 acttgcatgt gagtacctca agaaagaaac agaaggaaaa acaccaatta tggtttacgg
    31021 atgggtgccc acaacaccgg aagcctttta caaagatgtg gaaattgccc aacaggtggg
    31081 caccaatgag attctttatt gggaagctga ttacatagat aactccggag ctgacttcag
    31141 agccagtcac ttcagccgtt gtgtttccag ccaggcacca aatacaacac agtagcaggg
    31201 ggcggtatcg ccggaattgc cgctgcattg gcttcttcaa gaaacggagc aaaagtaatt
    31261 cttattgaaa agcaatgtgc tcttggcgga ctggctattt taggacttat tactatttat
    31321 cttccattgt gtgatggtat gggtaaacag gtaatttatg gaataggcga ggaattattg
    31381 cgtttatcaa taaaatacgg atatgaagat aattatccga atgcctggct ggataataat
    31441 aatattgaag aacgaaaaaa aacagcgttt tcttgtgcaa tataaccctc atcaaatagc
    31501 atgaaattga cgatgcagaa aatatagaag acagaggaca ttagggaaag tcataatttg
    31561 gataaggcaa gcagaggttt tatttttttt tggtaaattc tgctttataa aattttgtaa
    31621 aagtttgttg aaaaattagt aaaggctatt tacaaattat attactatga tgtaaaatga
    31681 atgtataggt tgtattaatt tacataggag gtagaatata tatatgaaag ggacggggat
    31741 cgtaagggaa gtagatgaat taggaagaat agttatcccg aaagaattga ggaaagttat
    31801 ggatattaag gtaaaagatc caatagagat ttttactgaa ggttcggcta ttatattgaa
    31861 gaaatatagc ccagggtgca tcttctgtga aagagtaaat gatgttataa gatttaaaga
    31921 gaataatata tgtaccgatt gtctaaaaca gttaaaaaac gatgactaat ttgcgaggat
    31981 taatgtacaa agattaaatt acgagaatta atgtataaag attaatgtac aagacactgc
    32041 ggagcgttgg caactaatgt tctgcggtac cagcaactct ctggaaaaca tttaataaat
    32101 aatttataaa taaataataa acaattaata aataaacaat aaataattaa taaatttatt
    32161 taatatttat taatgtcaaa tataaattac aacagtacag gagaaaaagc atcagaatat
    32221 taaaaagtaa cagaactgta tataaactca aaatatatta attacttcga aaagaataga
    32281 tactataaag aatcaggcga aaaaagcata taattaaact aaatgttcaa caatacagaa
    32341 atgcaaatat tttaaaaata atggtaaaat atatgtgata ttattaaata ttgtgttcaa
    32401 gcatagttat gcaggtgcat tttcttccta tgatacaaaa tatagcagta tacgattttc
    32461 cgagtataaa atttgaacaa agcttacgga ttgttatgtt taaggcaagg aaaaattcgt
    32521 aattcacgac acggatgtcg tgagccgtca cctgaatcat ggatgatgaa tgtgacgggt
    32581 agaatttttc tgtagactta aacactacaa tccgttgctg aagtgaatat tatacatgga
    32641 aaagggaata tttccacata tcgacatttg atattgacaa tacatgtatt ttaaggaatg
    32701 aagcacctga tgagatactg ggaaaattga ataaacactt ggtgaaaagc tatcgggagg
    32761 attatcggga agatcgccag caagattatc ggaagaatta ccagtaggat taccagaagg
    32821 attatcagca ggcttattag cagaattacc ggcatcatta tcagcatcat tatcagcatt
    32881 attatcaaca tgattatcag caagcttagc aacaagattg gggctaaagt acagcagaga
    32941 agccagcaaa acaagggcat ttgtaacata tacatcgcaa attctttgtg gatatacata
    33001 caagcagtta tgtaaatatg taggcaatat gtctttgtca gggatctcaa ggctctcaaa
    33061 caaagggttc taattattta aggaacatat aatatatcag gacgttatta ataccttgat
    33121 tggggtaaca taacatgaaa cataatagtt attgattatt gattatcgat ttgagtaaaa
    33181 tcacataaat tcaggcatca taataaacga atattcagcg aataatcaac gaatataaac
    33241 aaaatataat ataataatat atcaggaaag tgaagctcaa atataagctc attatttaca
    33301 ggtcttacat aaagtgtaaa ggtcttttcc ctattaaaaa agcaaaaaca aataaatatc
    33361 aaaaaatata tgttattatt aaatatatac aatatatatt tttgttatag agaggagata
    33421 taaaatgaag gtactattaa tgactgatct tgagggtatt agtggagtag ataccatgga
    33481 taaagtttcg gacgtgggaa ctcctggtta taggtttgcg cttgagcgcc ttatgcttga
    33541 tgtaaatgca gctgtcgagg gtgcttttga aggaggagca actgctgttt atgttaagga
    33601 tggtcatgga ggaggtaaca actttttgaa agaaatgttg gatccccgtg caattcaggt
    33661 atctgatagc ataagttggc agagactttt gaaagatggt gaaattgacg catatatgga
    33721 agttggtgca catgccatgg ctggtacaat aaatggtttc ctggatcata cacaaagctc
    33781 agtaaattgg cataactaca tagttaacgg aagaaagagc ggagagatag cacagggcgc
    33841 gatttttgcc ggagcatttg atgttccgtt tgttatggta agcggggatg aggcagcttg
    33901 cgttgaggcc agagctttcc tcgggaatat agagtgtgct gtagtcaaat atggtattgg
    33961 aagaaacaac gcaaggttag ttgatttaga tgaggcactt gaaaaaatta aagtcgctgc
    34021 tcgcgacagc ttaaaacttg ttggtaagat taaaccttat aaacctctat taccgttaga
    34081 aataatactt gaattgaacc gatcggatat gtgtgatgcc ttaatggaaa gatgtaagga
    34141 tgttgaacgg cttgatgccc gaactgtacg gaaacttgtg tacaaagtag agagttatat
    34201 tgatattttg ttttaag
//
LOCUS       DAOSDD010000016        33987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_647_length_33987_cov_5.297359, whole genome shotgun sequence.
ACCESSION   DAOSDD010000016 DAOSDD010000000
VERSION     DAOSDD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 33987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33987
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_33987_cov_5.297359"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1088)
                     /locus_tag="PK733_02605"
     CDS             complement(183..1088)
                     /locus_tag="PK733_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ09464.1"
                     /translation="MDDKAMKSRIMKKKWLLVIYIILFASILAGTVSCREKFSGKNKD
                     IEQIDHSEPRVKENVYLQGENIGGLTRSEALRIINEKALKVEKSPENAAIDNATWNIV
                     PEMSGLKVNIEETLQDALNAHENEEVKLKVYESIPETTKNSIEKNIVKIAEYTTPIVD
                     NTDSRMKNIWLAADKINNLVLAQGEEFSFNRVVGNRTEEKGYEYAPIIIKTEEGPKKE
                     DGIGGGVCQLSTTIYNAVEECGLEITERHTHSSEVTYVTPGEDAAVSYGSIDFKFKNN
                     RSYPIMLKINISPKALTVKVLENRN"
     gene            complement(1212..1670)
                     /gene="rimI"
                     /locus_tag="PK733_02610"
     CDS             complement(1212..1670)
                     /gene="rimI"
                     /locus_tag="PK733_02610"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOJ09465.1"
                     /translation="MKNIENVSIVRTDIKHIDDIILIEKLCFTIPWSRNAFIQELSSN
                     KFSIYISAMIDGKVVGYAGMWKIFDEGHITNIAVHPEYRQAGIGSMLLEGLINVAISE
                     GITRLTLEVRKSNAAAQTLYFKYGFEIEGCRKGYYADNGEDAIIMWRNKL"
     gene            complement(1677..2417)
                     /gene="tsaB"
                     /locus_tag="PK733_02615"
     CDS             complement(1677..2417)
                     /gene="tsaB"
                     /locus_tag="PK733_02615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOJ09466.1"
                     /translation="MKILAADTSSAVASVAVMEDSTLLGEYTLNHKKTHSQKLVPMIN
                     EILKNLELTPEEIDAFAVSIGPGSFTGLRIGVTTIKAMAYALQKQAIGVPTLDTLAFN
                     IPFCDSLICPIIDARNNQVYTSLYEWKGKKQERLSEYVGITIGELIDTVKAKNREAFF
                     VGDGVNVYKDTLLAELGNRCIFPPNNLLLQRASAVAEIAIHMQVDGKGFENEINTDKT
                     NENNLESWLTMVPFYLRKSQAERLFEQR"
     gene            complement(2414..2947)
                     /gene="tsaE"
                     /locus_tag="PK733_02620"
     CDS             complement(2414..2947)
                     /gene="tsaE"
                     /locus_tag="PK733_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOJ09467.1"
                     /translation="MLRISTVSDKETEEAGFYLGKVLVKGDIICLTGDLGAGKTVLVK
                     GIASGLGVDDYVTSPTFTLINEYEGEIPVYHFDVYRIADTEELYDIGINEYLYGDGII
                     LIEWADLIREILPEEVIWVNIDKCFSNTPQNQSGQNYSMYNNRIIYMEFRGEKYKKAE
                     EKYIGIMKIKGKEVVMG"
     gene            complement(3119..4411)
                     /locus_tag="PK733_02625"
     CDS             complement(3119..4411)
                     /locus_tag="PK733_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ09468.1"
                     /translation="MLLIKNGKILTMSGINYENGYIIIENGKIVEVGKDAGYLESKYF
                     NNDNRGNNGSNCGKSGSTDDGSDSVIDASGKYVLPGFIDAHCHVGMWEDSVGFEGSDG
                     NEATDPVTPQLRAIDGVYYMDRSFVEAREGGVTTVVTGPGSANVFGGQFAALKTYGKR
                     VEDMIVKEPIAVKIAFGENPKSVYKDRNQMPTTRMATAAILRENLKKAVEYKEALEEE
                     KKNEKTKAADKDTDTDKDVDKNKGKDKIKFDMKMDILVKVLNKEIPLKAHAHRADDIL
                     TAIRIAKEFDVNITIDHCTEGHLIKDILREEGISAIVGPFLTDRSKIELRNLSVKTPG
                     ILSRTGVKVAIMTDHPVIPIQNLWLCAAMAVREGMDEEEALKAITINAAQITGIDSRV
                     GSIEAGKDADIVIFDGHPFDIKSRVDFTIINGEKVYVR"
     gene            complement(4681..5958)
                     /locus_tag="PK733_02630"
     CDS             complement(4681..5958)
                     /locus_tag="PK733_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09469.1"
                     /translation="MKKTVKSIITFIMMLALSLTILMPANASNLDEAETKAAALKQLG
                     LFKGVSETNFDLNRAPTRTEALVMLIRLLGKESEALNGNWPHPFIDAESWADKYIGYG
                     YAKGLTKGVSNNKFGTGNADSDMYLTFVLRALNYNDTAGDFTWNAPDKLAAAVGILPD
                     GVDTGNFLRADVVLVSWAALEADLKGGTQRLAKKLLSEGIFTGDGYTRAIKLVNEQKP
                     EHVSISSAEALKAALSDKTVKVISIDSIGKPVVVTDELTIPARVTVTVNRGNDLYIEG
                     TLTNNGTINVMGADSITKDFINYSVMSVQNGGKIINNGVVNLHASLLEDTDDHGPVGG
                     QLRLFDGTFTNKGSVFLKAGLVNTHGGMVAVIDGTFNNDAIVIVDGFFLHVENGVFNN
                     NIGAVVINNSHIFAEETGKFTNNGTLSGNEINE"
     gene            complement(6102..7484)
                     /locus_tag="PK733_02635"
     CDS             complement(6102..7484)
                     /locus_tag="PK733_02635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ09470.1"
                     /translation="MNDMKIVVNDILNNFDNSAYPAGFLAKYHQLECLANSHGTETFL
                     VKPKDSEHLFVAKCYDKKLYSFVHEGSILKSLHHIGLPAFTDEFQNDTMVCIVREYIE
                     GKPLNQYIMENNPDNKNIINICLQLCNILIYLHGQEKPVIHRDIKPQNIIIKDDGQIV
                     LIDFDISRVYNSEAETDTQFFGTKEYASPEQYGFSQTDCRTDIYSFGVLLRFMLTENE
                     KENPDVHLYKPLEGIVKKCTAFAPKERFNNAAAVKKALLAADPKSQRIRKTLIAVCSV
                     AAIALCVLGSIKWYQYATHNPFAEGTIPAVLTDRERVADAVSYMSDKYDTDLFADTEA
                     YADIGFVKTILTDIYGYDSDYVHAMPEEGAPHESEKNFLPWGIGDEQYVARDVMVYVA
                     VKIYWVDKILDYSSLKDDNGYYPGVRVAVAFAEETGILTGVGRPEDITKGEVAILLAN
                     ADRVYEATKE"
     gene            complement(7481..7996)
                     /locus_tag="PK733_02640"
     CDS             complement(7481..7996)
                     /locus_tag="PK733_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09471.1"
                     /translation="MTFMLVKDGAEGSVSKKEVSNMDERTDQIRTSTLFRRLFKTSSL
                     EQFMKKNAGEMQLQSFSEYITELCEKQGEVPEHIIKRANIERSFGHQLFRGTRKPSRD
                     TVLQLAFGFDAGVETAQELLKHARMSALYPRVKRDVAIIYCLHNHFTIVETQRVLHEM
                     ELPLIGGGEVK"
     gene            complement(8348..8515)
                     /locus_tag="PK733_02645"
     CDS             complement(8348..8515)
                     /locus_tag="PK733_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09472.1"
                     /translation="MVQKSILFFVRFLAGLPDDVKISEEYLDFQQEGTVILRKEFSHT
                     PKKPSGLAFGK"
     gene            complement(8522..10945)
                     /locus_tag="PK733_02650"
     CDS             complement(8522..10945)
                     /locus_tag="PK733_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOJ09473.1"
                     /translation="MKKRFISVLLILCMMLTLLPTTAIADSTITATPSKTNFVINGQP
                     VSVTAAYTISGTNYLQLRAIAAMLNGTTAQFDVGWDGQYAVIEPGKPYSGTVTEVKLQ
                     NTTNLRQSDTKFKMNGEIFTFADARLIGGDTNYLQLREFAQKMGSTASKFNVYWDSKA
                     GQAVIEPGAVYTGNAPGIKNYNEEDVTWIGTTSEYKKTLAAFFDSGLRRVPVGDLAAD
                     GSLTNIKYGLVDANGAWAAQPIYDNIEAEYIYDKRSGEILRTFPNDHKPAESILVDGY
                     VQVTRGGKMGLLDSKGKEVIPCKYDAVGLPVEGISRLIAGSADKDTLGYPKRYYLGYW
                     SLEQGKEIVAPNKYVLPDIISATASGSPMIFSNSSFFGGDGGGYKYVSDIRIAAKFDF
                     NGGYALVPTAKTETLSFTFHDENGLPWQKDVSYHLTYFQMVDKNGKEALSQSYPYVTN
                     LLLGNYPQSGAYMVYEQVLTKKVWMVSDWKQNIILDKHIADGIVGAKGVIIPAQYHGG
                     IAGSEGAWVLNLASMQISSEKNVAVTTKCGHEKIKEGDFPLVVVDFNNKDVSSSFTVN
                     PYGLRNIDVSKVRFGEPWKDVDSDRNRWKIGVGTGIIGGTVAKNIANSQNALFDVVTG
                     KQLTPAFLVMRSMGRGLFVDTYGNNYGPDGKIVFPRAEKVTINDRGIYGNESGVGEDL
                     TLIVRDGNVGYINASRLARQGKGLPTTPRVKPAPPAIPVKAYFIQYPDKRTYKVGEGF
                     NTMGLIVHWQDDTGARTVINNSKLKFFTSGTVELTPGRAFTTKGVKAVEVLYNGIKVD
                     TFNVKVEGK"
     gene            complement(11182..11574)
                     /gene="rpsI"
                     /locus_tag="PK733_02655"
     CDS             complement(11182..11574)
                     /gene="rpsI"
                     /locus_tag="PK733_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ09474.1"
                     /translation="MDNVQYCGTGRRKKSIARVRLVPGDGRILINERSLDEFFGLETL
                     KVIVKQPLVLTDTIGKFDILCKVSGGGFTGQAGAIRHGISRALLEADEEFRTVLKKAG
                     FLTRDPRMKERKKYGLKKARKAPQFSKR"
     gene            complement(11599..12033)
                     /gene="rplM"
                     /locus_tag="PK733_02660"
     CDS             complement(11599..12033)
                     /gene="rplM"
                     /locus_tag="PK733_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOJ09475.1"
                     /translation="MKTYMAKPAEVKRSWYVVDAEGKPLGRLASEVAKILRGKNKPEY
                     TPHVDVGDYVIIINADKVVLTGKKLDQKYYRRHSGFPGGMKETLYRNLIESNPEKAIE
                     VAVKGMLPKNSLGRAMSRKLKVYRGSEHNHQAQKPENLVLSI"
     gene            complement(12346..13197)
                     /gene="truA"
                     /locus_tag="PK733_02665"
     CDS             complement(12346..13197)
                     /gene="truA"
                     /locus_tag="PK733_02665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOJ09476.1"
                     /translation="MRNIKLTIEYDGTNYNGWQSQKNGNTVEDMVREAIYLLTGEKVD
                     VIGSGRTDSGVHAYGQVANFYTQSSIPADKFSFALSSKLPCDIVIRESVEVGSDFHAR
                     FSAKGKKYRYLLYNSPHPSALLRNRTYHVFYNLNIEAMKEASCFFLGTHDFVGFMAKG
                     SNVKTTVRTIKEINVRRQSPILTVDEQSPIFIEKQSTITAGGQSPITTSGLSHMQKEN
                     GLIQIEITGDGFLYNMVRIIVGTLIEVGIGKLKSGDIKNIIESCNRVNAGRTVPACGL
                     YLVEVYY"
     gene            complement(13219..14163)
                     /locus_tag="PK733_02670"
     CDS             complement(13219..14163)
                     /locus_tag="PK733_02670"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HOJ09477.1"
                     /translation="MYYIGIDLGGTNIAAGLVNEEGIIVLKDSVPTLREREYPEIIRD
                     MAMLSLKLIKDAGIDIKEVKSIGIGAPGTPDNKNGILVYANNLKFRNVPMRAEMQKHV
                     NLPVFIDNDANCAALAESVAGAAKGVENSITVTLGTGIGGGLVISGKVYPGFNGAAAE
                     VGHIVIISEGKECTCGRKGCWEAYGSATALIERTQDAARENPDSLINKLVDGDLSKVN
                     AKTAFDAARQGDKTGVEVVDRYIRYVAEGIIDLINIFTPEILVIGGGVCKEGEFLLKP
                     LREHIAEGVYARDVPQTQLKVAVMGNDAGIIGAAMLGK"
     gene            complement(14183..14983)
                     /locus_tag="PK733_02675"
     CDS             complement(14183..14983)
                     /locus_tag="PK733_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOJ09478.1"
                     /translation="MIRDITLGQYIPGNSPIHRADPRVKMVLSIIYMILIFIVNNYMG
                     FLILYLFTAFVVTVSDIPIKYTLKGLKPIALIVIFTMAVNIFTVGGRTVFEYGFIKIT
                     YEGIDLSIKLALRLSLLVITTSLLTLTTTPILLTDAIESLMSPFKFIGIPVHEIAMMM
                     TISLRFIPTLMEETDKIMKAQSSRGADFDTGNIFQRAKSFVPILVPLFISAFRRADEM
                     AMAMEARCYRGSEGRTRMKQLKITSIDYKIMAVMMVVALGLLYLQYMK"
     gene            complement(14976..15848)
                     /locus_tag="PK733_02680"
     CDS             complement(14976..15848)
                     /locus_tag="PK733_02680"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOJ09479.1"
                     /translation="MSIIIEGLSYIYMKGSPFSREALEDINLKIDQGEFIGIIGHTGS
                     GKSTLIQHLNGILKPTAGRVIVNGIDTSGKALRELRKQVGVVFQYPEHQLFEETVYKD
                     IAFGLKQSGVDPARIDRKVREVLEVVGLQEDILEKSPFELSGGQKRRIAIAGVLVMEP
                     SILVLDEPAAGLDPKGRNEIFKFIRELYDDSGITVILVSHNMEEIAKIVQRVIVMNNG
                     RIEMDGSIKEIYNNVERLESIGLSAPQITYLMKKLRKVYPDIEDNIFTVEEARKEILR
                     KIRANKVDLGDNHD"
     gene            complement(15865..16728)
                     /locus_tag="PK733_02685"
     CDS             complement(15865..16728)
                     /locus_tag="PK733_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOJ09480.1"
                     /translation="MNNNFIEIKNISHIYKTDATDNGVEALSNISINIEKGSFVVILG
                     RNGSGKSTLAKLMNVLIMPSEGAILVKGIDTREQNNTWEIRRIVGMVFQNPDNQIIGT
                     IVEEDVAFGPENLGVPPGEIVKRVEKALNVVGLTDYNNNAPHMLSGGQKQRVAIAGIL
                     AMKPECIVLDEATSMLDPVGRKEVMNVLRKLNKEEDITIVHITHNMDEACMADRVVVI
                     NEGTVRVDGTPGEVFSNVDMVKNLGLDVPQVTELFYELEKEGCKLPKGVLTVDEAFDI
                     LVRKINKNESL"
     gene            complement(16871..17395)
                     /gene="rplQ"
                     /locus_tag="PK733_02690"
     CDS             complement(16871..17395)
                     /gene="rplQ"
                     /locus_tag="PK733_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOJ09481.1"
                     /translation="MPQQRKLGRPTDQRVAILRGLVTALLTNGRIETTEARAREVKNI
                     TESLIALAIREADKFSSKQIKISAAKVDSTGKKITKSVTSKNNRKYEVVERAEKTDMI
                     TVDNPSRLHARRQIIKWVNRVKDENGKQINVVDKLFDEIAPKYKDRNGGYTRIYKLGP
                     RRGDAAERVILELV"
     gene            complement(17416..18363)
                     /locus_tag="PK733_02695"
     CDS             complement(17416..18363)
                     /locus_tag="PK733_02695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ09482.1"
                     /translation="MLEIEKPRIECAELSEDNLYGRFIIEPLERGYGITLGNSLRRVL
                     LSSMPGVAVTSIKIDGVLHEFSYIPGVVEDITEIILNIKELAVKLHSEGPKVVYIDAE
                     GEGPITAADIKADADVEVTNPDLHIATLNGESRFYMEMVLNRGRGYVSADKNKLSGQP
                     IGIIPVDSIYTPVKKVNYLVENTRVGQDTDYDKLVFEVWTNGTIEPDEAISFGAKILS
                     EHLNLFIDLSEQAKHTEIMVEKEETKKEKVLEMTIEELDLSVRSYNCLKRAGINTVED
                     LISKTEDDMMKVRNLGRKSLEEVLQKLSGLGLSLAKSED"
     gene            complement(18513..19139)
                     /gene="rpsD"
                     /locus_tag="PK733_02700"
     CDS             complement(18513..19139)
                     /gene="rpsD"
                     /locus_tag="PK733_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOJ09483.1"
                     /translation="MARYTGPSCKLCRREGEKLFLKGERCYTDKCSVGRRGYAPGQHG
                     QQRRKLSEYGMQLREKQKARRFYGVLEKQFRKYFDMATNMKGVTGENLLKLLESRMDN
                     VVYRLGFANSRPEARQLVKHNHFTVNGKKVNIPSYLLNPGDVVAVAEKSKNSEKIKSI
                     VENTSGKIIPGWLELDLDNLSGKVVTLPSREEIDLPVKEYLIVELYSK"
     gene            complement(19209..19610)
                     /gene="rpsK"
                     /locus_tag="PK733_02705"
     CDS             complement(19209..19610)
                     /gene="rpsK"
                     /locus_tag="PK733_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOJ09484.1"
                     /translation="MATKTAGKRTTRKRRERKNIERGAAHIRSTFNNTIVTITDTAGN
                     AVSWASSGGLGFRGSKKSTPFAAQMAAETAAKAAMEHGMKTVEVYVKGPGSGREAAIR
                     ALQAAGLEVNLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(19633..20004)
                     /gene="rpsM"
                     /locus_tag="PK733_02710"
     CDS             complement(19633..20004)
                     /gene="rpsM"
                     /locus_tag="PK733_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOJ09485.1"
                     /translation="MARIAGVDLPREKRVEIGLTYIFGIGRSSSKNILTKTGVNPDTR
                     VKDLTDDEISRLRDVIEKELKVEGDLRREVSLNIKRLIEIGCYRGRRHRQGLPVRGQR
                     TKTNARTRKGPSKTVGVQRKK"
     gene            complement(20125..20238)
                     /gene="rpmJ"
                     /locus_tag="PK733_02715"
     CDS             complement(20125..20238)
                     /gene="rpmJ"
                     /locus_tag="PK733_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOJ09486.1"
                     /translation="MKVRPSIKTMCEKCKIIRRKGSVRVICENPKHKQKQG"
     gene            complement(20258..20476)
                     /gene="infA"
                     /locus_tag="PK733_02720"
     CDS             complement(20258..20476)
                     /gene="infA"
                     /locus_tag="PK733_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOJ09487.1"
                     /translation="MSKEGVIEVEGKVVEALPNAMFSVELENGHKVLAHISGKLRMNF
                     IRILPGDKVNLELSPYDLSRGRIVWRAK"
     gene            complement(20480..20776)
                     /locus_tag="PK733_02725"
     CDS             complement(20480..20776)
                     /locus_tag="PK733_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOJ09488.1"
                     /translation="MDYSLGELVYSKAGRDADRKFIVVKIVDEKYILISDGCLRKIEK
                     PKKKQNKHVVSSGVVIDSLNKKLINNARVTNAEIRKEIELHVQYLETLEKTRRV"
     gene            complement(20850..21617)
                     /gene="map"
                     /locus_tag="PK733_02730"
     CDS             complement(20850..21617)
                     /gene="map"
                     /locus_tag="PK733_02730"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOJ09489.1"
                     /translation="MITIKSQYEIDIMKKAGEITANALEKIRQAITPGITTLEIDRIA
                     EDYIKSKGAQPSFKGYKGLPGAMDFPASICASVNNEVVHGIPGLRMLKDGDIISIDIG
                     VYFNGYHGDAARTYPVGTVSDEAKKLIEVTKESFYEGIKYAVERNRIVDISAAIQKYV
                     ERCGYSVVREYVGHGIGREMHEAPQVPNYRTRERGPRLQQGMTLAIEPMVNQGGYQVK
                     ILDNRWTVVTADGSLSAHYENSILITNDQPYILTINK"
     gene            complement(21618..22265)
                     /locus_tag="PK733_02735"
     CDS             complement(21618..22265)
                     /locus_tag="PK733_02735"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOJ09490.1"
                     /translation="MRIVLLGAPGAGKGTQADVLSEKFNIPHISTGDIFRCNIKNGTD
                     LGKKAKEYIDKGLLVPDEVTASIVKSRLMENDCVNGFILDGFPRTIPQAEFLDKVLNE
                     LGVKLNYVLNIYVPEQKIVERLSGRRVCEKCGKSYHIIYNPTSREGICDDCNSKVVQR
                     IDDNEETVLNRLKTYHQQTEPLIQYYEKQGKLVTATGQDRIEDTIKEVLKALGAE"
     gene            complement(22485..23777)
                     /gene="secY"
                     /locus_tag="PK733_02740"
     CDS             complement(22485..23777)
                     /gene="secY"
                     /locus_tag="PK733_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOJ09491.1"
                     /translation="MGGMLQTLKNAWNIKELRKKLMFTIIMLVVFRVGSHIPVPWLNA
                     EKFRSLIGQGGQLFGFLDIIAGGAFSNATIFAMSIQPYINASIIINLLTVAIPKLEKL
                     AKEGEEGRKIIAQYTRYGTVILGFLQATALYLGMRSAVTPGSPVLSYITITLSFTAGT
                     AFLMWLGEQITEYGIGNGISILIFAGIVSRAPQAVISFVRLYIGKAFGTGIFSIITII
                     LILFVFVVVIAAVIWVQEAERRIPIQYAKRVVGRKVYGGQNTHLPIKVNLAGVIPIIF
                     AMSIMAFPSTIIGLIAPNSTNKFALWLKNAQQSVLYAVLYALLIIFFTFFYTIIQFNP
                     VEIANNMKKNGGFIPGIRPGKPTADYISKVLNRITWFGGVFLAAITLLPNLLGIIFKV
                     QGIWFSGTAMLILVGVAMDTIKQIESYMLMRNYKGFLE"
     gene            complement(23779..24222)
                     /gene="rplO"
                     /locus_tag="PK733_02745"
     CDS             complement(23779..24222)
                     /gene="rplO"
                     /locus_tag="PK733_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOJ09492.1"
                     /translation="MKLHELKPAPGSKKLPNRRGRGPGSGNGKTGGRGHKGQKARSGG
                     GVRIGFEGGQMPLYRRVPKRGFNNKIFAKQYSEVKLSDLNIFEDGTVVNWESLLENGI
                     VKNVYDGIVILGNGEITKKLTVQAARFTKTAAEKIEAAGGKVEVV"
     gene            complement(24237..24416)
                     /gene="rpmD"
                     /locus_tag="PK733_02750"
     CDS             complement(24237..24416)
                     /gene="rpmD"
                     /locus_tag="PK733_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOJ09493.1"
                     /translation="MAKLKITLKRSMCNIKPNQVATIKALGLRKPGSCVEHEDTPQIR
                     GMIKSVEHLVSVEEV"
     gene            complement(24429..24932)
                     /gene="rpsE"
                     /locus_tag="PK733_02755"
     CDS             complement(24429..24932)
                     /gene="rpsE"
                     /locus_tag="PK733_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOJ09494.1"
                     /translation="MQRIDPNIFEELKEKVVNINRVAKVVKGGRNFRFTALVVVGDEN
                     GHVGAGLGKAAEIPDAIKKGIEDAKKNLINVSLVETTIPHEVMGEYGAGKVLLKPAGT
                     GTGVIAGGPVRAVLELAGIRDIRTKSLGSNNPTNMVRATINGLSQLKTVEEVAKLRGK
                     TMEEILG"
     gene            complement(24948..25310)
                     /gene="rplR"
                     /locus_tag="PK733_02760"
     CDS             complement(24948..25310)
                     /gene="rplR"
                     /locus_tag="PK733_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOJ09495.1"
                     /translation="MIQKSNSNKDRQKKHLRIRNKVFGTTERPRLNVFRSLNNIYAQI
                     IDDTTGMTLVAASTLDIKGKINYGGNKEAAKEVGRCIGERALEKGIKRVVFDRGGYIY
                     HGRVKELADAAREAGLEF"
     gene            complement(25348..25899)
                     /gene="rplF"
                     /locus_tag="PK733_02765"
     CDS             complement(25348..25899)
                     /gene="rplF"
                     /locus_tag="PK733_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOJ09496.1"
                     /translation="MSRIGRMPVAVPKGVNVTVNGNTVTIKGPKGTLTKEFHKDMKIE
                     LDNDVITIKRPSDDKLHKSLHGLTRSLVNNMVIGVTKGFEKALDVSGVGYRAQKQGKK
                     LVINLGYSHPVEMEEPEGITIEVPAPNKIIVKGCDKQAVGECAAKIRSKRAPSAYLSN
                     NAVKGIKYEKEVVRTKVGKAGAK"
     gene            complement(25970..26368)
                     /gene="rpsH"
                     /locus_tag="PK733_02770"
     CDS             complement(25970..26368)
                     /gene="rpsH"
                     /locus_tag="PK733_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOJ09497.1"
                     /translation="MQTTDAIADMLTRVRNASTAKHEIVDIPASNIKKEIAKILRDEG
                     YIKNIEYIEDNKQGIIRITLKYSANKKSIITGIKRISKPGLRVYAEKDEVPKVLGGLG
                     IAIMSTSKGVVTDKVARENGVGGEVLAFIW"
     gene            complement(26398..26583)
                     /locus_tag="PK733_02775"
     CDS             complement(26398..26583)
                     /locus_tag="PK733_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOJ09498.1"
                     /translation="MAKKSMILKQSRKSKFSTRAYNRCKLCGRPHAYIRKYGVCRLCF
                     RELAYKGQIPGVKKASW"
     gene            complement(26601..27143)
                     /gene="rplE"
                     /locus_tag="PK733_02780"
     CDS             complement(26601..27143)
                     /gene="rplE"
                     /locus_tag="PK733_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOJ09499.1"
                     /translation="MNRLKEKYISEVIPGLQARFKYSNPMQLPKLDKIVINMGVGDAR
                     ENPKAIDAAAGDLAAIIGQKPVITKAKKSVAAFKVREGMNIGCKVTLRGKRMYEFADK
                     LFNIALPRVRDFRGVPANSFDGRGNYSLGIKEQLIFPEIVYDQVDKIRGMDITFVTTA
                     RTDEEAKELLKLLGMPFSHS"
     gene            complement(27170..27520)
                     /gene="rplX"
                     /locus_tag="PK733_02785"
     CDS             complement(27170..27520)
                     /gene="rplX"
                     /locus_tag="PK733_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOJ09500.1"
                     /translation="MANKVHVKTGDMVYVLSGKGRGKKGKVLKVLPKEKRVVVQGVNI
                     VKKHQKPRSMYQQGGIVEKEGTISSSNVMLICPRCNKPTKIGKDVMENGQKARVCKKC
                     SEVIDVIKEAKDDK"
     gene            complement(27539..27907)
                     /gene="rplN"
                     /locus_tag="PK733_02790"
     CDS             complement(27539..27907)
                     /gene="rplN"
                     /locus_tag="PK733_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOJ09501.1"
                     /translation="MIQTQSMLKSADNTGAKDMMCIKVLGGSKRKYANIGDIVVASVK
                     NATPGGVVKKGEVVKCVIVRSKKGLKRSDGSYIKFDENAAVIIRDDKNPKGTRIFGPV
                     ARELRDKDFMKILSLAPEVL"
     gene            complement(27996..28256)
                     /gene="rpsQ"
                     /locus_tag="PK733_02795"
     CDS             complement(27996..28256)
                     /gene="rpsQ"
                     /locus_tag="PK733_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOJ09502.1"
                     /translation="MQVDRGRRKTRVGKIVSDKMDKTVVVAIETSVKHPLYKKVVKRT
                     YKLKAHDENNECKEGDKVRVMETRPLSKDKRWRVVEIIEKAK"
     gene            complement(28340..28549)
                     /gene="rpmC"
                     /locus_tag="PK733_02800"
     CDS             complement(28340..28549)
                     /gene="rpmC"
                     /locus_tag="PK733_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOJ09503.1"
                     /translation="MKANEIREKTQQELNQELTELKSELFKLRFQHATNQLDNPMRLK
                     EVKKSIARVKTIMRERELKEVKTAK"
     gene            complement(28539..28976)
                     /gene="rplP"
                     /locus_tag="PK733_02805"
     CDS             complement(28539..28976)
                     /gene="rplP"
                     /locus_tag="PK733_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOJ09504.1"
                     /translation="MLMPKRVKFRRVQRGRMKGVATRGNQVSNGEYGLKALEPAWITS
                     NQIEAARIALTRFIKRGGQVWIKVFPDKPVTKKPAETRMGSGKGSPEYWVAVVKPGRI
                     LFEIAGVSEEDAREALRLAAHKLPLKCKFVVRGQEMGGEANES"
     gene            complement(28976..29641)
                     /gene="rpsC"
                     /locus_tag="PK733_02810"
     CDS             complement(28976..29641)
                     /gene="rpsC"
                     /locus_tag="PK733_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOJ09505.1"
                     /translation="MGQKVNPHGLRIGIIKDWETKWYAGDKNFSEFLIEDFKIREYLK
                     KKLYISGISRIEIERFANKIRININTAKPGLVIGKGGSGIEELRKEIEKMTNKGILIN
                     ISEVKVPELDAQLVAENIASQLEKRISFRRAMKQAMSRTMKLGAKGIKTSVGGRLGGA
                     EIARTEHYHEGTIPLQTLRADIDYGFAEANTTYGKIGVKVWIYKGEVLPAKKVKKVEG
                     GER"
     gene            complement(29661..30137)
                     /gene="rplV"
                     /locus_tag="PK733_02815"
     CDS             complement(29661..30137)
                     /gene="rplV"
                     /locus_tag="PK733_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOJ09506.1"
                     /translation="MEKKVMSKDELLKQKDKLLEIYNSTHSKSRKPVLLTKKEKRILG
                     IGKDEGRASLSFARISSRKVKIVLDLIRGKDLDEAYGMLKNTPKAASDILLKLLKSAE
                     ANAVNNNDLDRDKLYVAEVFANQGPSLKRVRPRARGSAFKIRKRTSHITLVLKERK"
     gene            complement(30172..30456)
                     /gene="rpsS"
                     /locus_tag="PK733_02820"
     CDS             complement(30172..30456)
                     /gene="rpsS"
                     /locus_tag="PK733_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOJ09507.1"
                     /translation="MSRSLKKGPFVDSKLLKKIEEMNANNDKKVLKTWSRASTIFPQM
                     VGHTIAVYDGRKHVPIYITEEMVGHKLGEFAPTRTFRGHGHHTEKSTSLK"
     gene            complement(30507..31337)
                     /gene="rplB"
                     /locus_tag="PK733_02825"
     CDS             complement(30507..31337)
                     /gene="rplB"
                     /locus_tag="PK733_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ09508.1"
                     /translation="MALKKYSPTSPGRRNMTVSTFEEITDNKPQKSLLGTKNKNGGRN
                     ANGRITVRYRGGGSRQKYRVIDFKREKDGVAAKVASIEYDPNRSANIALLHYVDGEKR
                     YILAPLGLKVGDTVESGVNADIKPGNALPLANIPLGSLIHNIELKPGKGAQIVRSAGN
                     YAQLMAKEGNYAQVRLPSGEVRMVSMVCRATIGQLGNIDHENISLGKAGRTRWLGRRP
                     KVRGSAMNPVDHPHGGGEGKAPIGRPSPVTPWGKPALGYKTRKKNKKSDKYIIKSRNG
                     "
     gene            complement(31379..31732)
                     /gene="rplW"
                     /locus_tag="PK733_02830"
     CDS             complement(31379..31732)
                     /gene="rplW"
                     /locus_tag="PK733_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOJ09509.1"
                     /translation="MMHFEDIIVRPHITEKSNLEAAGGKYTFIVNAKAKKTEIKQAVE
                     KLFQVKVIWVNTVNYKGKTKRLGVHEGKRPNWKKAIVQIDINPEAETYLAKGGKLVGL
                     NKKYKESIEEFGAIQ"
     gene            complement(31732..32355)
                     /gene="rplD"
                     /locus_tag="PK733_02835"
     CDS             complement(31732..32355)
                     /gene="rplD"
                     /locus_tag="PK733_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOJ09510.1"
                     /translation="MPKIDLLNISGKKVGEIELNNDIFGVDVDPLVLHMAVVNQLANK
                     RQGTQSTKTISEVSGGGRKPWRQKGTGRARQGSIRSVQWIKGGIALGPKPRSYSYKLP
                     RKVRRIALKSALSSKVNTDNIIVLDELKFDQIKTKQMVDVLKNLDVNSSALIVLSDKN
                     EKVERSAGNIPNIKTALVNTINVYDILKYDKFIITKDAVEKVEEVYA"
     gene            complement(32377..33018)
                     /gene="rplC"
                     /locus_tag="PK733_02840"
     CDS             complement(32377..33018)
                     /gene="rplC"
                     /locus_tag="PK733_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOJ09511.1"
                     /translation="MKKFIIGKKLGMTQLFDEEGLCIPVTVVKAGPCTVVQKKNKEND
                     GYNSLKLGFEDIKEKRVIKPQRGEFSKYKISPKKYLKEFRVDDSGKFENGQEIKVDDM
                     FVQGDRVDVTGISKGKGFQGVIKRFGLARGRETHGSMYHRRVGSMGAGTDPGRVFKRK
                     KLPGHMGAIKVTVQNLDVVRVDTERNLLLIRGAVPGPKGGLLIIKETVKAGSK"
     gene            complement(33172..33483)
                     /gene="rpsJ"
                     /locus_tag="PK733_02845"
     CDS             complement(33172..33483)
                     /gene="rpsJ"
                     /locus_tag="PK733_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOJ09512.1"
                     /translation="MANNQKIRIRLKAFDHQLLDQSAEKIVETAKRTGASVSGPVPLP
                     TEKEITTILRAPHKYKDAREQFEMRTHKRLIDILRPTPKTVDALMRLDLPAGVDIEIK
                     L"
BASE COUNT         8981 a         7827 c         4903 g        12276 t
ORIGIN      
        1 cattttagat tgcaagcctg atttattatt tattattact tctgccatcg tcatatttgt
       61 ttaccctcct aatataccaa cttattataa atatatatat tcatattata tcaaagaaaa
      121 taataaaagt ggtatattta tttattcagc gtgggaaatc attacaattc ctcataaata
      181 cttcagtttc tattttccaa aactttcact gtcagtgctt tcgggcttat attaatcttt
      241 aacattatag ggtaacttct gttgttttta aacttaaaat ctattgaccc ataggatact
      301 gcagcatctt ctcccggggt aacatatgtt acttcagaag aatgagtatg tctttccgtt
      361 atttcaaggc cgcattcttc taccgcatta taaatagttg tagaaagctg gcaaacccct
      421 ccacctatac catcttcctt tttaggtcct tcttctgtct taattattat tggagcatat
      481 tcatatcctt tttcctccgt tctgtttcct actactctgt tgaaagaaaa ctcttctcct
      541 tgagccagaa ctaaattgtt tattttatct gccgccaacc aaatattctt cattcttgaa
      601 tcggtgttat caacaattgg agtggtgtat tccgctattt taactatatt tttctcaatg
      661 gaattttttg tggtttctgg aattgactcg taaaccttca atttaacttc ctcattttca
      721 tgggcattca atgcatcttg aagtgtttct tctatgttaa ctttcaatcc ggacatttca
      781 ggaacaatat tccatgtagc attatctatg gctgcattct ctggactttt ttccaccttt
      841 aacgcctttt catttataat tctcagagct tctgatcttg tcagccctcc tatattttcg
      901 ccctgcaaat aaacattctc tttaactctg ggttcactat ggtcaatctg ctctatgtcc
      961 ttattcttac ctgaaaactt ttccctacat gacacagtac ctgctaagat tgatgcaaac
     1021 aaaattatat atattaccaa taaccatttt ttcttcataa ttctggattt cattgcttta
     1081 tcgtccatac aaaacacacc tccgtgtgct tagtatggat gataaagcat caattattcc
     1141 tgtggatact tgttgtaacg catcattata aaaattttcg gttgtagaaa aatttcccgg
     1201 cattcgcctc gttatagttt atttctccac attataatag catcctcacc attatcagca
     1261 taatatcctt ttctacatcc ttctatttca aatccgtact taaagtacaa tgtttgtgct
     1321 gccgcgttgc tttttctcac ctcaagggta agtcttgtga tcccttcact tatggcaaca
     1381 ttaattaagc cttctaaaag catgcttcct attccagctt gtctatactc aggatgaaca
     1441 gcaatattgg taatatgtcc ctcatcaaat attttccaca taccagcata tccaactact
     1501 tttccatcaa tcatagctga aatatatatg gaaaacttgt tgctgctcag ttcctgtatg
     1561 aaagcatttc ttgaccaggg aattgtaaaa cacagctttt ctatcaatat tatatcatca
     1621 atatgcttaa tgtctgtcct gacaatgctt acattttcta tgttcttcaa gttctatcac
     1681 ctttgttcaa acaatctttc agcttgagac tttctaagat aaaaaggaac catggtcaac
     1741 caactctcta aattgttttc attagtttta tctgtattta tctcattttc aaatcctttt
     1801 ccatcaactt gcatatgtat ggcaatttct gctactgcag atgctctttg caataaaagg
     1861 ttatttggag ggaaaataca tctattaccc aattctgcta aaagtgtatc cttgtataca
     1921 ttaactccat ctccaacaaa aaatgcttct ctattttttg ccttaactgt atctattaat
     1981 tcacctatag ttattcccac atactcactt agtctttcct gctttttacc cttccactca
     2041 tataaggaag tatatacttg attatttctt gcatctatta tgggacatat aagggaatca
     2101 caaaaaggta tgttaaaagc aagggtatca agggttggaa ctcctattgc ctgtttttgc
     2161 aaagcgtaag ccatagcttt gatagtagta acaccaattc ttaaacctgt gaaagaacct
     2221 ggacctattg aaactgcaaa ggcatctatt tcttcaggag tcaattctaa attttttaat
     2281 atctcattaa tcattggtac aagtttttga gaatgtgttt ttttatgatt taatgtatat
     2341 tcacctaata gtgtagaatc ctccataaca gccacagaag ctacagcaga agaagtgtct
     2401 gccgccaata ttttcatccc attactacct cttttccctt aattttcatt atgcctatat
     2461 acttttcttc ggcctttttg tatttttccc ctctaaactc catatatatt attctattat
     2521 tatacataga ataattttgt ccactttgat tctgtggagt attagaaaaa catttatcaa
     2581 tattcaccca tattacttcc tcaggaagta tttctcttat taaatcagcc cactctatta
     2641 aaattatacc atcaccatat aagtactcat taattcctat atcatacagc tcttctgtat
     2701 ctgcaatcct gtagacatcg aagtggtaca caggaatttc accttcatac tcgttaatca
     2761 atgtaaaggt aggacttgta acataatcat ctactccaag tccagatgct attcccttta
     2821 caagaactgt ctttcctgcg ccaagatcac cggtaagaca tattatatcg ccttttacca
     2881 atacttttcc aagataaaaa cctgcttctt cggtctcttt atccgacact gtgcttatcc
     2941 ttaacaaatt ttcactcctt atcaattctc ccaacttaaa atctaccttt accctctaac
     3001 ctcttccccc cccaactacc cactacccgc tacccactac ccaactctaa cctctaacct
     3061 ctaacctttt aggcgcctgc tcggaaagtc ctggctataa aagcggacgc ctaacttctc
     3121 accttacata taccttttca ccattaataa tagtaaaatc aacccgtgat tttatatcaa
     3181 aaggatgtcc atcaaaaata acaatatctg catctttacc cgcttcaata cttccaaccc
     3241 tgctgtcaat acctgttatc tgagcggcat ttattgttat tgcttttaaa gcttcttctt
     3301 catccatgcc ttcccttaca gccatagctg cacaaagcca gagattctgt atcggaataa
     3361 caggatggtc tgtcataata gctaccttta cgcctgttct agaaagtatt cccggtgttt
     3421 ttacgcttaa gtttctaagt tcaattttcg accggtctgt aaggaacggt cccactatag
     3481 ccgatatacc ctcttctctc aaaatatcct taataagatg cccttcagta cagtgatcta
     3541 ttgttatatt tacatcaaat tcttttgcta tcctgattgc ggtaagaata tcatccgctc
     3601 tgtgtgcatg agctttcaaa ggtatttcct tgtttaggac ttttacaaga atgtccattt
     3661 tcatatcgaa tttaatttta tcttttcctt tatttttatc tacatcttta tctgtatctg
     3721 tatctttatc tgcagcttta gttttttcgt tctttttctc ctcttccagg gcttctttgt
     3781 attccacagc ttttttcaaa ttctctctaa gtattgcagc agtagccata cgagtagtag
     3841 gcatttgatt tctgtcttta tatacacttt taggattttc accaaaagca attttcaccg
     3901 ctataggttc ttttacaatc atatcctcta ctcttttgcc gtatgtttta agggctgcga
     3961 actgccctcc gaacacgttg gcactgccag gacctgtaac aacagttgta acaccgcctt
     4021 ctcttgcttc cacaaacgat ctgtccatat agtaaacacc atctatagca cgtaattgcg
     4081 gtgttacagg atctgtagct tcatttccat cagacccctc aaatcctacg gagtcttccc
     4141 acatacctac atggcagtga gcgtcaataa atcccggcag gacatacttg ccggaagcat
     4201 ctataacact atctgaacca tcatcagtgc tacccgattt gccacaatta ctaccattat
     4261 tacctctatt atcattatta aaatacttgc tttccaaata tccagcgtct ttacctactt
     4321 ctactatctt tccattttca ataataatat atccattttc gtaattaatg cctgacatag
     4381 tcaggatttt cccatttttt atcagcaaca taatgccacc tcacattttt gtacttatta
     4441 tataatattc attttaatag acaaagcaac gactttcaag agtaatcacg aaaaccgggt
     4501 gcattttcta tatttgaggg aaaatatggc aagaaacgat ttccatgtat aatattcatt
     4561 tcagcaacgg attgtagcgt ttaagtatac agaaaaattc cggtatgcaa taattagtta
     4621 ccggaatttt tgatttgata tgtcgcctat ttcaaattgt gctgtagcat tgcggctaaa
     4681 tcactcgttg atctcattcc ccgaaagagt gccgttattt gtaaatttcc cggtttcttc
     4741 cgcaaaaata tggctgttgt taattacaac tgcaccaatg ttattgttaa aaactccatt
     4801 ttcaacatga agaaagaacc catctacaat aactatagcg tcattgttaa aagtaccatc
     4861 gatcacagca accataccac cgtgagtgtt gacaagtcca gcctttagaa aaacggaacc
     4921 tttattggtg aaagtgccgt cgaatagacg gagttgtcca cctacgggcc cgtggtcgtc
     4981 tgtatcttca agcagggatg catgcagatt taccacacca ttgtttataa tctttccgcc
     5041 gttctgaacg gacattacgg aatagtttat aaaatccttg gtaatagaat cggcacccat
     5101 cacattgata gtaccattgt ttgtaagggt accctcgata tacaaatcgt ttccgcgatt
     5161 gactgttacc gtgactcgtg cgggtatggt aagttcatca gtaacaacta caggtttccc
     5221 tatggaatca atggatataa ccttcacagt tttgtccgat agagctgcct taagcgcctc
     5281 agcagaggaa atggaaacat gttcgggctt ttgctcgttt accagcttga ttgcacgtgt
     5341 ataaccgtcc cctgtgaata taccttccga tagtaatttc ttcgcaagcc tctgggtacc
     5401 gcccttcaga tctgcttcca atgcagccca tgaaacaagt accacatctg cacgtaaaaa
     5461 atttcctgta tccacaccat caggaagaat tccaactgct gctgcaagct tatccggtgc
     5521 attccaggta aaatcaccag ctgtatcatt ataattcagt gcacggagca caaaggtcag
     5581 atacatatcc gaatctgcat tacctgtgcc gaatttatta ttggaaacac ccttggtaag
     5641 tccctttgcg tagccataac caatatattt atcagcccat gattctgcat ctataaacgg
     5701 gtgcggccag ttgccattca gagcttctga ttcttttcca agtaaccgaa tcagcattac
     5761 cagtgcttcc gtgcgggtag gtgccctatt cagatcaaag ttggtttcag acacgccttt
     5821 aaataatcca agctgtttaa gcgctgctgc tttcgtctct gcctcatcta aatttgacgc
     5881 atttgccggc ataagtattg taaggctcaa ggccagcatc ataataaaag taataatgct
     5941 ttttactgtt ttcttcatat ttcggtctcc tttttttaaa tttactttat actgcttata
     6001 aattgtcaat ctactattga catcacgcca tcatcatttt acaaaataga aaaagaaaaa
     6061 aggtgagctg ccagcccacc tttcgagaaa attagatttt tttattcttt tgtcgcctcg
     6121 tacactctgt ctgcgtttgc aagaaggatt gcaacttcac ctttggtaat atcttcagga
     6181 cgtcctactc ctgtgagtat tcctgtttct tcagcaaaag caaccgccac gcgaacacct
     6241 ggataataac cattgtcatc tttcagactt gaataatcca aaatcttgtc tacccaatat
     6301 atttttacag caacatagac catgacatca cgggcaacat attgttcatc ccctattccc
     6361 caaggaagaa agtttttttc gctctcatga ggtgcacctt cctccggcat ggcgtgaaca
     6421 taatcgctgt cgtatccata tatatctgtt aatatagttt ttacaaagcc aatatctgca
     6481 tacgcctcag tatcagcaaa cagatctgta tcatatttat cactcatata agaaacggca
     6541 tcggcaactc tttccctgtc tgtcaaaaca gcagggatcg ttccctcagc aaatgggtta
     6601 tgggtcgcat actgatacca cttgatgctg cccaacacac acagcgcaat tgccgcaaca
     6661 gagcaaacgg ctatgagtgt tttccgtatt ctctgtgatt ttggatctgc cgcaagcaaa
     6721 gcctttttca ctgctgcagc attgttaaag cgctcttttg gagcaaatgc ggtacacttt
     6781 tttacaatac cttccagcgg cttatacaaa tggacatctg gattttcttt ttcgttttct
     6841 gtgagcataa accgaagcaa aacgccaaag gaataaatgt cggtgcggca atccgtttgt
     6901 gaaaagccat attgctccgg cgacgcatac tccttcgtgc cgaaaaactg agtatcggtt
     6961 tccgcttcac tgttataaac tctggaaatg tcaaagtcaa tgagaacaat ctgtccatca
     7021 tccttaataa ttatattctg tggcttgata tccctatgga taacgggctt ttcctgtcca
     7081 tggaggtaga tgagaatgtt gcaaagttga aggcagatat tgatgatgtt tttattgtca
     7141 ggattatttt ccattatata ttggttgagg ggtttaccct cgatatattc gcgcactatg
     7201 cagaccatcg tgtcattttg aaattcatct gtaaatgcag gcaaacctat atgatgcaga
     7261 gactttaaga tactgccctc atgcacgaaa gagtagagtt ttttatcata acatttcgct
     7321 acaaaaagat gttcgctgtc ttttggtttt actaaaaaag tctctgtacc gtggctattg
     7381 gcaaggcact ccaactgatg gtattttgcc aaaaaccctg caggatatgc cgaattatca
     7441 aagttgttca gaatatcgtt aacaactatt ttcatatcat tcattttact tcgccgcctc
     7501 caatcaaagg taattccatt tcgtgtaaaa ctctttgtgt ttccacaata gtaaagtggt
     7561 tatgcaggca gtagatgatt gccacatcac gttttacacg tggatacaag gcactcattc
     7621 tggcatgttt taaaagttcc tgtgctgttt ccacaccagc gtcgaatcca aaggccaatt
     7681 gtagaactgt atcccgtgat ggcttccttg tccctctgaa aagttgatgc ccaaatgagc
     7741 gttcaatatt tgctcgcttt ataatatgct ccggcacttc gccttgtttt tcacacagtt
     7801 ccgttatgta ttcactgaaa gactgtaact gcatttctcc tgcattcttt ttcatgaatt
     7861 gttctaaact tgatgttttg aataagcgtc taaataatgt acttgttctg atttgatctg
     7921 tcctttcatc catattgctc acttctttct ttgatactga cccttctgcg ccatctttaa
     7981 ccagcataaa tgtcatttgt tgtgcctccg cattgtatcc ttaaatcatt agataaaaaa
     8041 ccgccaaggg ttgatccccc cctgacggtt tctgttttta acaagcaaga tttgtaattc
     8101 ttaacaaact tgcttggtaa ttgataataa ttatcccttt gataaccggt gttctttgat
     8161 taaatagtac catattttgc ttatatatgc aagagaaaac ttcaatcttt ttcaaaaact
     8221 ttttctggat tctttgacat tcgctttggt tttttcttgt tatagattgg cagcgataga
     8281 tcaggaaaaa gagcatctca tttcatactt ggtatatccg ccttggcgga tttctccacc
     8341 agatagtcta tttgccgaaa gccaagccac ttggcttttt tggtgtatga ctgaactctt
     8401 ttcttaatat aacagtccct tcctgctgaa aatctaaata ttcctcggaa atcttaacat
     8461 catccggcaa tcccgctaaa aaccgtacaa aaaacaaaat tgacttttgt accattgttg
     8521 ctcatttgcc ttcaaccttg acattaaatg tatcaacttt tattccgtta tatagaacct
     8581 caaccgcctt taccccttta gttgtaaatg cccggcccgg cgtaagctcc actgtacccg
     8641 aggtaaaaaa tttgagcttt gagttgttga taacggttcg agcaccggta tcgtcttgcc
     8701 aatgcacaat aagtcccatt gtattaaagc cctcgcccac cttgtacgtt cgtttgtcag
     8761 gatactggat aaaataggcc ttaacaggaa tggcaggcgg tgcaggtttt actcggggtg
     8821 tggtcggaag tcccttgcct tgtctcgcca accttgaggc attgatatag cccacattgc
     8881 catcccgaac tataagagta aggtcttctc cgacgcctga ttcgttgccg tatattcctc
     8941 tgtcatttat cgttactttc tccgctcttg gaaatacaat ctttccatcc ggtccatagt
     9001 tattgccata tgtatcgacg aacaaaccgc gtcccattga gcgcataact aaaaatgccg
     9061 gagttaattg cttccctgtg accacatcaa agagagcgtt ttgactgttg gcgatatttt
     9121 ttgcaacagt gcccccgata attccggtgc caaccccgat tttccatcga ttgcgatcgg
     9181 agtcaacatc cttccaaggt tcaccgaatc ttactttaga tacatctata tttcttaacc
     9241 cgtagggatt aacagtaaac gaagatgaca cgtctttatt gttaaagtcc acaacgacta
     9301 agggaaagtc gccttctttg atcttttcgt gaccgcattt tgtggttaca gctacatttt
     9361 tttcagatga aatctgcata gaggcgaggt taagtaccca agcgccctca gaccccgcaa
     9421 taccgccgtg atattgtgcg ggtataataa cacctttagc cccgactatg ccgtctgcaa
     9481 tgtgtttgtc caaaattata ttttgtttcc agtcggaaac catccacacc ttttttgtca
     9541 agacttgctc ataaaccata tatgctcccg attgcgggta gttacccagc aataaattcg
     9601 ttacatatgg gtaggattgg gacaacgctt cttttccatt tttgtcaacc atctgaaagt
     9661 acgttaagtg atagctcaca tctttttgcc atggcagtcc attttcgtcg tgaaaggtaa
     9721 atgataaagt ttctgttttc gcagtgggaa ccagtgcgta tccgccattg aaatcaaact
     9781 tcgcggcaat acggatatca cttacatatt tgtaaccacc gccgtcgcca ccaaagaagg
     9841 agctgttgct gaaaatcatt ggactgccac tggcagtggc gctaattata tcagggagaa
     9901 catatttatt gggtgcgacg atttctttgc cctgctctaa gctccagtag ccaaggtagt
     9961 atctcttcgg ataaccaaga gtgtctttgt ccgctgaacc tgcaataaga cggctgatgc
    10021 cctccaccgg cagacccacc gcatcatatt tacagggaat gacttccttg cctttgctgt
    10081 caaggagacc catcttcccg ccacgggtta cctggacata tccgtcaacg agtatacttt
    10141 cagctggttt atgatcatta ggaaatgttc ttaaaatttc gccggagcgt ttatcataaa
    10201 tgtactctgc ctctatattg tcgtagatag gctgggccgc ccacgcgcca ttagcatcaa
    10261 ccagaccgta cttgatattg gtcaggcttc catcagccgc caagtcgccc acaggcactc
    10321 tgcgaagccc tgagtcaaag aatgccgcaa gggtcttctt gtactccgaa gtagtgccaa
    10381 tccatgtcac atcttcttca ttatagttct taatccccgg agcattccct gtgtagacag
    10441 cacccggctc aatgacagcc tgacctgctt tactgtccca ataaacattg aacttagatg
    10501 cagtgctacc catcttttgt gcgaactcgc gtagctgaag gtagttggta tctccgccaa
    10561 tcagccgggc gtccgcaaag gtaaagattt caccattcat cttgaacttt gtgtcgcttt
    10621 gtcgaaggtt tgttgtgttt tgaagcttaa cttctgtaac cgtgccactg taaggctttc
    10681 caggctcaat cacggcatac tggccgtccc atcccacgtc gaactgtgcg gtagtcccgt
    10741 taagcatcgc cgcgatggcg cgaagctgca aataattggt gccacttatg gtgtatgccg
    10801 ctgtgacgga tacgggctga ccattaatca caaaattcgt cttgcttggc gtggcagtaa
    10861 tcgtactgtc cgctatcgcc gtcgttggta gcaaagtcag catcatgcac aggataagta
    10921 gaacgcttat aaatcgcttt ttcatagacc cgtccctccc atatggattt ttgctttcat
    10981 catatcagaa acttcctgaa aaaaagccgt ccgctgcatg aaacatcgtt ttttcgacat
    11041 gaatcatagt ctcggaaatt cagtttttat ggtaaactta gcttggagaa aggaaagcgg
    11101 tttgatgagt tttattagga tcgtaagcat aaaaaaatcc cacaaacatg cgtgtttgtg
    11161 ggattttgtt tttctgaaat cctatctctt tgagaactgt ggtgcctttc ttgctttctt
    11221 gagaccatat ttctttcttt ccttcattct ggggtctctt gtcaaaaaac cggctttttt
    11281 aagtacagtt ctaaattctt catcagcttc gagtaatgct ctcgatatgc catgtcttat
    11341 tgctcctgcc tgtcctgtaa aaccaccccc gctaaccttg cacagtatat caaactttcc
    11401 gatagtatct gtcaatacta aaggctgttt tactataact tttaaagttt caagtccaaa
    11461 aaattcatcc aggcttcttt cattaattaa tatcctgcca tctccaggaa caagtcttac
    11521 tcttgcgata gatttttttc ttctaccagt gccacaatat tgtacattgt ccatataatt
    11581 cctacgcctc ccttcttatt aaatacttaa tacaagattc tcaggtttct gggcctggtg
    11641 gttatgttca ctacccctgt aaaccttaag cttcctggac attgctctac ctagactgtt
    11701 tttcggcaac atacctttaa cagcaacttc tattgctttt tcaggatttg actctatcaa
    11761 attcctgtat aatgtttcct tcattccccc tggaaatccg gaatgacgtc tgtagtactt
    11821 ctggtcaagc tttttacctg tcaagacaac cttgtcggca ttaataatta ttacataatc
    11881 gcctacatct acatgtggtg tatattccgg cttgtttttg cctctgagaa tttttgccac
    11941 ttcacttgcc agtctgccta atggcttgcc ttctgcgtct acaacatacc aacttctttt
    12001 tacctctgcg ggcttagcca tgtatgtttt cattcaacaa taaacctcct taaacttctt
    12061 ttctcccata ataaatttat atttgaatta gctttcatat caacattaaa gcactataac
    12121 attgtaatac aggtatgtgc tgcatgtcaa gtacaaattg ctgataattt aatttatctc
    12181 ttatttactt catattataa caattgcatc ctgttttctt tctaataatc gtccgctatt
    12241 tcactttttc aaccttctat aggggaaatt aacttctaac ctcccacttt tagcctataa
    12301 cctttaggag cctgctcgaa aagtctaagc tattaaagtt gactcctagt aatacacttc
    12361 caccaaatac aatccgcagg caggtacggt tcttcctgca tttaccctgt tgcagctttc
    12421 aatgatattt ttaatatctc cagattttag tttgccaata cccacctcaa ttaatgtacc
    12481 aacaataatt ctaaccatat tataaagaaa accatcacct gttatttcta tctgaataag
    12541 cccgttctct ttctgcatat gggacaatcc gcttgtagtt attggagact gcccccctgc
    12601 agttattgtg gactgcttct ctatgaatat tggggactgt tcatctacgg ttaaaattgg
    12661 tgactgtctc cttacgttta tttccttaat tgtcctgaca gtagttttta cattgctgcc
    12721 cttggccata aagccgacaa aatcatgagt acccaggaaa aagcaagaag cttctttcat
    12781 ggcttcaata ttgagattat agaacacatg atatgtacgg tttctaagta atgcagaggg
    12841 atgaggggaa ttatagagta aatacctata cttttttccc tttgcagaaa atcgagcatg
    12901 aaaatcggat ccaacttcta cagattctct aataactata tcacatggca atttgctact
    12961 taatgcaaaa gaaaacttat ctgccggaat gcttgattgt gtataaaaat ttgctacctg
    13021 cccatatgca tgaacaccgg aatcagttct accggagccg ataacatcta ctttttcccc
    13081 tgttagcaaa taaatagcct ctcttaccat atcttcaaca gtatttccat ttttctgact
    13141 ctgccagcca ttgtaatttg taccgtcata ttcgattgtt agctttatgt tacgcactag
    13201 aaccaccgta tttcatcttt acttaccaag catcgcagcg cctataattc ctgcatcatt
    13261 acccatcact gcaactttta attgagtttg cggtacatct ctggcataaa cgccctcagc
    13321 tatatgctct cttagaggct taagcaggaa ttcaccttct ttgcacactc ctccgcctat
    13381 aacaaggatc tctggagtaa aaatatttat aaggtctatt ataccttcag ctacatatct
    13441 tatgtagcgg tcaacaactt caacccctgt tttatcgccc tgccttgcag catcaaatgc
    13501 agttttggca tttactttgc ttaaatctcc atctaccagt ttgtttatta aagaatcagg
    13561 attttctctt gcagcgtcct gggttcgttc aattaatgca gttgcagatc cataggcttc
    13621 ccaacatcct tttctaccgc aagtacattc ttttccttct gaaattatga ctatgtgccc
    13681 aacctcggca gcagctccat taaatcctgg atatacttta ccactaatta caagtccccc
    13741 gcctatacct gttccaagtg ttactgtaat agagttctct acaccttttg ccgcacctgc
    13801 aacgctttcg gccagtgcgg cacaattagc atcattatca ataaaaacag gtaagttaac
    13861 atgtttttgc atttctgctc tcatggggac atttctgaat ttaaggttat tagcatacac
    13921 caaaataccg tttttattat caggggtacc aggcgcacct attccaatgc ttttaacttc
    13981 cttgatatca atccctgcat cctttatcag cttcaatgaa agcattgcca tgtccctgat
    14041 aatttcagga tattcacgct ctcttaatgt aggaacacta tctttaagaa ctattatgcc
    14101 ctcttcattt actaatccag cagctatatt agttcccccc aggtcaatac caatataata
    14161 cataatctac actcctccca tattatttca tatattgtaa atacaacaat cctaatgcca
    14221 ctaccatcat tactgccatt attttataat ctatagaagt aattttcaat tgtttcatcc
    14281 ttgttcttcc ttcacttccc ctatagcatc ttgcttccat tgccatagcc atttcatcgg
    14341 cacgccggaa agcactaata aaaagaggca ccaggatggg cacgaagctt ttagccctct
    14401 gaaaaatatt acccgtgtca aaatcagcac ctcttgaact ttgtgccttc attatcttgt
    14461 cggtttcttc catcaaagtg gggataaacc tcagtgaaat tgtcatcatc atagcaattt
    14521 cgtgtacagg tattcctata aacttaaatg gactcataag actttcaatt gcatcagtaa
    14581 gtaatatagg agtagttgta agtgtaagca gtgatgttgt gatgacaagc aatgaaagcc
    14641 ttaacgccag ctttatggat aaatcaattc cttcgtaggt tatttttata aaaccatatt
    14701 caaaaacagt tcgtcctcct actgtaaaaa tattaactgc catagtaaat ataactatta
    14761 gagctatagg ttttaaacct tttaatgtgt acttgattgg aatatcagaa actgttacta
    14821 caaacgcagt aaatagatat agtattaaaa atcccatata gttattaact ataaatatca
    14881 agatcatata tattatggaa agaaccattt taactcttgg atcagcccta tgtatgggtg
    14941 aattgccagg aatatattgt ccgagcgtaa tatctctaat catgattatc acctaaatca
    15001 accttgttag ccctaatttt tctcaatatt tcttttcggg cttcttctac agtaaatata
    15061 ttgtcttcaa tatcagggta tactttcctt agtttcttca tcaggtaagt aatttgtggt
    15121 gctgaaagcc ctatactttc aagcctttct acattattat atatttcttt tatactcccg
    15181 tccatctcaa ttctgccatt attcattact attactcttt gcactatttt ggctatttct
    15241 tccatgttat gggatacaag tattactgtt ataccggaat catcataaag ctctcggata
    15301 aatttaaata tttcatttct tccttttggg tcaagccctg ctgctggttc atccagtaca
    15361 agtatggaag gctccattac taaaacacct gctattgcaa ttctgcgttt ctgtcccccc
    15421 gaaagctcaa aaggtgattt ttcaagtatg tcttcttgaa gtccaactac ttctaatact
    15481 tctctgactt ttctatcaat cctggctggg tctacacctg attgtttcaa gccaaaggca
    15541 atatctttat atactgtttc ttcaaacaat tgatgttcag gatattggaa aactactcca
    15601 acctgttttc taagttccct aagtgctttc cctgatgtat caataccatt tactattacc
    15661 ctgcctgctg taggtttaag tataccattt aggtgctgta tcagagttga ttttccagag
    15721 cctgtatgac caattatacc tataaattcg ccctgatcaa ttttaagatt tatatcttcc
    15781 agtgcttctc tggaaaaggg gctgcctttc atataaatat atgataatcc ttctataata
    15841 attgacatta cggctagacc tccgttataa cgactcattt ttattaatct ttcttacaag
    15901 tatatcaaaa gcttcatcta ctgtaagaac accttttggc agtttacagc cttccttttc
    15961 aagttcataa aacaattccg taacctgtgg aacatcaagc cccagatttt taaccatgtc
    16021 cacatttgaa aacacttctc cgggtgttcc atcaaccctg acagtccctt cgtttataac
    16081 tacaaccctg tcagccatac atgcttcatc catattatga gtaatgtgga ctatggtaat
    16141 atcttcttct ttattcaact ttctcaaaac gttcattacc tctttgcgtc caacaggatc
    16201 aagcattgaa gtagcttcat caaggactat acactctggt ttcattgcaa gaataccagc
    16261 tatagccact ctctgttttt gccctcctga aagcatatgg ggagcattat tgttgtaatc
    16321 tgtcaagcct acaacattta gggccttttc tactctcttt acaatctccc ccggtggaac
    16381 ccccaaattc tcaggtccaa atgcaacatc ttcttctacg atagtaccaa ttatttgatt
    16441 atcaggattt tgaaaaacca ttcctactat acgtcttatc tcccaagtat tgttttgttc
    16501 tcttgtatca atccctttta ctaatattgc accttccgag ggcattatta aaacgttcat
    16561 aagttttgcc aaagtggact tgcctgaacc atttctaccc aatattacta caaaacttcc
    16621 cttttcaata tttatactaa tatttgataa tgcttcaacc ccattatcag ttgcatccgt
    16681 tttgtaaata tggcttatgt tttttatttc aataaaatta ttattcatcg taaattcccg
    16741 ccaaagtagt aatatatttt ttgaaacata caaaataagg gacaaggcat acttccttat
    16801 acctaatccc ttacccccac cttccaagtg ttacttcaat ctgctctcta aaacggtgtg
    16861 tcccattagc ttataccaat tcaagaataa ctctttctgc agcgtctcct cttcttggcc
    16921 caagcttata aattcttgta tatccgccat tgcgatcttt atattttgga gcaatttcat
    16981 caaataactt atctacgaca tttatttgtt ttccattctc gtcctttact ctattgaccc
    17041 attttataat ttgccttctg gcatgcagtc ttgagggatt atcaacagtt atcatatctg
    17101 ttttctctgc tctttctaca acttcatatt ttctgttgtt tttagatgta acagattttg
    17161 ttattttctt acctgtacta tccacttttg ctgcactaat ctttatttgt tttgatgaga
    17221 atttatcggc ttctcttatt gcaagagcga ttaaactctc tgtaatattt tttacctctc
    17281 tagcccttgc ttctgtcgtt tcaattctac catttgtcag tagagctgta accagacctc
    17341 tcaatattgc tactctttgg tcagtagggc gtcccaactt tctctgctgc ggcataatca
    17401 tcaacctccc ctgctttaat cttcactttt tgctaaagaa agccccaatc cacttaattt
    17461 ctgaagtact tcttcaaggg atttccttcc aaggtttcta accttcatca tgtcatcttc
    17521 agtcttgctt ataaggtctt caaccgtatt aatacctgct ctctttaagc agttatagga
    17581 ccttacagac aagtcaagtt cctcgatagt catctccagt actttttcct tcttggtctc
    17641 ttccttttca accattattt ctgtatgttt tgcctgctct gataaatcga taaaaaggtt
    17701 caaatgctca cttaatattt ttgctccaaa acttattgct tcatcaggct ctattgttcc
    17761 atttgtccat acttcaaata ctaatttatc atagtctgta tcctgtccaa cacgtgtatt
    17821 ttcaaccaaa tagttaactt tctttacagg tgtgtatata gaatcaacag gtataatacc
    17881 aataggctga cctgatagtt tgtttttgtc cgcagatacg tatccacgac ctctatttaa
    17941 gaccatttcc atgtaaaatc tgctctcgcc atttaaagtt gcaatatgca gatcagggtt
    18001 ggttacctca acatcagcat ccgctttaat atctgccgct gtaataggtc cttctccttc
    18061 tgcatcaata taaactactt ttgggccttc actatgaagc tttacagcta attcctttat
    18121 attcagaatt atttctgtaa tatcttctac tacaccggga atgtaagaga attcatgtaa
    18181 cactccgtct atttttattg atgttactgc aactccaggc atcgaagaaa gcaacacacg
    18241 cctgagagaa tttcccagtg ttattccata tcctctttca agaggttcaa ttataaatct
    18301 gccatataaa ttatcttcac taagctcagc acattcaatt cttggtttct ctatttcgag
    18361 cacttaaaac cctcccttat tgcactttta ttaatgaggg caactaattt gtaaatactt
    18421 tctgctactt gatctctgct aaagatacct ctcattttta atactatacc tcggtcatta
    18481 taacaattat tatatgctca atactcatat tattacttgg aatacaattc aacaataagg
    18541 tattccttaa caggcaggtc gatctcttct ctggagggaa gtgttactac ttttccgctc
    18601 aagttatcga gatcaagctc taaccagcct ggtataatct taccagatgt attctcaaca
    18661 atactcttta ttttttccga gtttttgctc ttttcagcta ccgctacaac atcaccggga
    18721 tttaaaaggt atgacggtat atttactttt ttaccattta ctgtaaaatg gttgtgtttt
    18781 accaattgtc ttgcctcagg tcttgaatta gcaaatccca gcctgtaaac tacattgtcc
    18841 atgcggcttt ccaataattt taaaagattt tcaccggtaa cacctttcat atttgttgcc
    18901 atatcaaagt atttcctaaa ctgtttttca agtactccat aaaatctacg agctttttgt
    18961 ttttctctta attgcatacc gtattcagaa agttttcttc tctgctgtcc gtgctgtccg
    19021 ggggcataac ctctcctccc aactgaacac ttatcagtat aacatctttc acctttcaga
    19081 aaaagctttt ctccttctct acggcacaat ttacaagatg ggcctgtata tcttgccatc
    19141 tgaattagca cctccaataa atatctgttt cattttaatt tgatacactt gcatttgtta
    19201 taatactatt aaactcttct tctcttgggt ggtctgcatc cgttgtgagg aattggtgtt
    19261 acatctttta tcagatttac ttcaagacct gcagcttgca aagctcttat tgctgcttct
    19321 ctcccggacc ccggcccttt tacataaact tctacagtct tcattccatg ttccatagct
    19381 gcttttgcag ctgtttcggc tgccatctgg gctgcaaatg gagtgctttt tttagaacct
    19441 ctaaagccta acccacctga acttgcccat gataccgcat ttcctgcagt atcagttatt
    19501 gtaacaattg tattgttaaa cgttgatctt atatgtgcag ctccacgctc tatatttttt
    19561 ctttctcttc tttttcttgt agtcctcttt ccagcagttt tagttgccat ttagcttctt
    19621 acctcctttt cactactttt tcctctggac acctacagtt ttgctgggac ctttacgtgt
    19681 ccttgcatta gtttttgtcc tctgccctct aacaggaagt ccctgtctat gtcttctacc
    19741 cctgtaacag ccaatttcaa taagcctttt aatatttagg gaaacctctc ttcttaaatc
    19801 tccctcaact ttcagctctt tttcaattac gtccctgagt ctgcttattt catcatcagt
    19861 aaggtcctta actcttgtat cgggatttac tcctgttttt gtcaatatat tttttgaact
    19921 ggatctgcca ataccgaata tataagtcag tcctatttct accctttttt ctctgggtaa
    19981 atcaactccg gcaatacgcg ccattgattt ctacacctcc atcatttcaa ctgtatacat
    20041 aatattagtt taccttttta tatgaacata acggatcatg atttctcatg cagccgcacc
    20101 cgcatgtttt ttcacaaatt taagctaacc ctgcttttgc ttatgttttg gattttcaca
    20161 aattaccctt acactacctt ttcttcttat tatcttacat ttttcacaca tggtttttat
    20221 agatggcctt actttcacag ttatccctcc ttaatagtta ttttgctctc catacaattc
    20281 tcccacgtga tagatcatag ggagacagtt caagattcac cttgtctccg ggcaatatac
    20341 gtataaaatt catacgcaat ttcccggaaa tatgagctaa tactttatgt ccattctcaa
    20401 gttcaaccga aaacatagca ttgggcaatg cctctactac tttaccttca acttcaatta
    20461 cgccttcctt agacaaaatt taaaccctcc ttgtcttctc aagagtttca agatactgaa
    20521 catgaagttc aatctctttt cttatttccg catttgtcac tcttgcatta tttatcagct
    20581 ttttatttaa agaatcaata acaaccccac tcgatacaac atgcttattt tgtttcttct
    20641 taggtttttc aatttttcta aggcagccat ccgaaattag tatatatttt tcatcaacta
    20701 tcttaacaac tataaatttc ctgtcagcat ctctacctgc tttagaatac accaattccc
    20761 ccagggaata atccattgtt ttcacctcta cttgcttggg gcgaaatttt attattttct
    20821 accaagtaaa taaaaataat tatatacttt tatttattta tggtcaatat atatggttgg
    20881 tcattagtaa ttaaaatgct gttttcgtaa tgtgctgaca gacttccgtc agcagttact
    20941 acagtccatc tattatctaa tatcttaacc tggtaacccc cttgatttac cattggttcg
    21001 atagccaggg tcattccctg ctgtaacctt gggcctcttt ctcttgtcct gtagttgggc
    21061 acttgaggtg cttcatgcat ttctcttcct ataccatgcc caacatattc ccttacaact
    21121 gaatacccac atctttcaac atatttttgt attgcagctg aaatatcaac tatacgattt
    21181 ctttcaactg catacttaat accttcatag aaactttcct tagttacttc aattagtttt
    21241 ttggcttcat cggatactgt ccctactggg tatgttcttg ctgcatcacc atggtacccg
    21301 ttgaaatata ctccaatatc aatactaata atatctccat cttttaacat tcttaaaccg
    21361 ggtatcccat gaactacctc attattaaca gaagcacata tgcttgctgg aaaatccatt
    21421 gcaccgggta aacctttata acctttaaaa gaaggttgtg caccttttga tttaatgtag
    21481 tcttcagcta ttctatcaat ttccaaggta gttattccag gagttattgc ctgcctaatc
    21541 ttttctaaag catttgcagt aatttcgcct gcttttttca ttatatcaat ttcatattga
    21601 gattttattg ttatcattta ttctgcaccc agtgctttca atacttcttt aattgtatct
    21661 tctatcctat cctggcctgt agcagtaaca agttttccct gtttctcata atactgtatt
    21721 aatggctcag tctgttgatg gtaagtctta agcctgttta aaacagtttc ttcattatca
    21781 tcaattcttt gtactacttt tgaattacaa tcatcacaaa ttccctctct ggatgtagga
    21841 ttgtatatta tgtgataact ctttccacat ttctcacata cccttcttcc agatagtctt
    21901 tcaactattt tttgttctgg tacatatata tttagtacat aatttagttt aacacctagt
    21961 tcattaagaa ccttatcaag gaattcagct tgtggtatag tcctgggaaa gccatcaaga
    22021 ataaagccat taacacaatc attttccata agtctactct ttacaatact tgcagtgact
    22081 tcatcaggaa caagcaatcc tttatctata tattcttttg cttttttacc taaatctgta
    22141 ccatttttta tgttacatct aaatatatct ccagtagaga tatgaggtat attaaatttt
    22201 tctgataata cgtctgcctg ggtaccttta cctgcacctg gagcacctaa caatacgatt
    22261 ctcatagcca tatcagctcc tattttttaa aaactaattt ccagctgtga acctggcttt
    22321 atttattctt aatgtatctt atgacattat caatttgcag tttctatatt ttgacttata
    22381 actgtaaatt tcccacatct atcttaccac ttgccatttc taacttccaa cctctaatct
    22441 ctaacactaa cctctatccg ctaaccatta accactatct acttctattc aaggaatcct
    22501 ttatagtttc tcatcagcat atatgattca atttgtttaa ttgtatccat tgcaactccc
    22561 acaagaatca acatagctgt gccgctaaac catattccct gtactttaaa tattattccc
    22621 agcagatttg gtaaaagtgt tattgctgca aggaacactc ccccgaacca tgttattcta
    22681 tttaatacct ttgatatata atccgcagtt ggttttcctg gtctaatacc tggtatgaac
    22741 ccaccatttt tcttcatatt attagcaatt tcaacaggat taaactgaat aattgtatag
    22801 aaaaatgtaa aaaatattat taataaagca tataacactg catataaaac actttgttgt
    22861 gcattcttaa gccataatgc aaatttattt gttgaattcg gggcaataag acctattata
    22921 gtggaaggga atgccattat tgacatggca aaaataattg gtattacacc tgccaagttt
    22981 acttttatag gtaaatgggt gttttgtccc ccatatactt ttcttcctac aactcttttg
    23041 gcatactgta tgggaattct tctttcagct tcctgtaccc atataactgc tgctataact
    23101 acaacaaaaa caaataatat taatataata gtaattatac taaaaattcc tgtaccgaaa
    23161 gcttttccaa tatacagtct tacaaatgat ataactgcct gtggagctct ggaaactata
    23221 ccggcaaata ttagtattga tattccattc cctattccgt attcagttat ttgttctcca
    23281 agccacatca agaaagctgt gcctgctgta aatgatagtg ttattgttat ataacttaat
    23341 acagggcttc ctggtgttac agcacttctc ataccaaggt aaagagctgt tgcctggagg
    23401 aaacctaaaa taactgttcc atatcttgtg tattgggcaa ttatttttct tccctcttcg
    23461 ccttctttag caagcttttc taatttaggt attgccactg taaggaggtt aataataatt
    23521 gaagcattaa tgtatggttg tatgctcatg gcaaatatcg ttgcattaga aaatgcacca
    23581 cctgcgataa tatctaaaaa tccaaaaagc tgtccacctt gaccaataag acttctgaat
    23641 ttttctgcat ttagccatgg cactggaata tgtgaaccta ctctgaatac tactagcatt
    23701 ataatagtaa acattagttt tttgcgtaat tcctttatat tccaggcgtt ttttaatgtt
    23761 tgaagcattc ctcccatact ataccacctc tacctttcct cctgcagctt ctatcttctc
    23821 agctgctgtc ttagtaaatc ttgcagcttg aacagttaac tttttagtta tctcaccatt
    23881 accaagtata acaataccat cgtaaacatt tttaacaata ccattttcaa gtaaagattc
    23941 ccaatttacc acagtaccgt cttcaaaaat gttcagatct gataatttaa cttctgagta
    24001 ttgttttgca aatattttat tgttaaagcc tctctttggt actcttctgt aaagaggcat
    24061 ctggccacct tcaaagccaa ttcttactcc tccgccagac ctggcttttt gacccttatg
    24121 acctcttccg ccagtcttgc cattacctga acctggacct ctgcctcttc tatttggaag
    24181 tttttttgaa ccaggagcag gttttaattc atgcagtttc acgtcttata cctccttcaa
    24241 acttcttcca cactaacaag atgttcaaca ctttttatca tacctcttat ttgcggagtg
    24301 tcttcatgct ccacacaact acctggcttt cttaatccca gcgcctttat ggtagcaact
    24361 tgatttggtt ttatattaca catacttctt tttaaagtta ttttcaattt tgccactaaa
    24421 actcccccct agccgagtat ttcttccata gtcttaccac ggagttttgc aacttcttct
    24481 acagttttta attgagaaag accatttata gttgccctta ccatatttgt tggattattg
    24541 gaaccaagag atttagttct tatatcccta attcctgcca attcaaggac agctcttaca
    24601 ggtccacctg ctataactcc ggtaccggta cctgcaggtt ttaacaatac tttacctgca
    24661 ccatattctc ccataacttc atggggaatt gtagtttcta ctaacgaaac atttatcagg
    24721 ttcttttttg catcttcaat tccttttttt attgcatcag gtatttctgc cgcttttcct
    24781 aatcccgcgc ctacatgtcc attctcatcg cctactacta caagagctgt aaaacgaaaa
    24841 tttctgccac ctttaacaac tttggcaaca cggtttatat taacaacctt ttctttgagc
    24901 tcttcaaaaa tatttggatc tatacgctgc aatttacttc cctccttcta gaattccaac
    24961 ccggcttctc ttgctgcgtc agcaagttct tttactcttc cgtgatatat atatccgcca
    25021 cgatcaaata caactctttt aatgcccttt tccagtgctc tttctcctat gcacctgcct
    25081 acttcctttg cagcttcttt atttccaccg tagttgattt ttccttttat atccagagta
    25141 gaagcagcta caagagtcat tccggtcgta tcatctataa tctgtgcata tatattattc
    25201 aggcttctaa agacatttaa tcttggtctt tcagtagttc caaaaacttt atttctaatc
    25261 cttaaatgct ttttttgcct atctttgtta ctattagatt tttgtatcat tttctttcaa
    25321 ctcccttctg gtaatctttc acacttctta cttggctcct gctttaccta cttttgtcct
    25381 tacaacttcc ttctcatact taataccttt tacagcatta tttgagagat aagcggacgg
    25441 tgctctcttg cttcttattt tagctgcaca ttcaccgact gcctgcttgt cacatccctt
    25501 tacgataatt ttattagggg caggaacttc aatagtaata ccttccggtt cttccatttc
    25561 taccggatgg gaataaccca agttaattac cagtttcttt ccctgcttct gggctctata
    25621 tccaactccg ctgacatcca atgccttttc aaatcctttg gttactccta taaccatatt
    25681 gtttacaagg cttcttgtta aaccatgtaa agatttgtgc agtttatcat ctgaaggtct
    25741 ctttattgta ataacatcat tatctaactc tattttcata tctttatgaa attcttttgt
    25801 caaagtgcct tttggccctt ttatcgttac tgtattgccg ttaaccgtta cattaacacc
    25861 ctttggtact gctaccggca tcctccctat tcttgacata tactttcctc ctgctcttaa
    25921 cattgtgagt gttttttcca ctcttttcag aaatatttaa tactttaatt taccatataa
    25981 atgccaatac ttctccgcct acaccgtttt ccctagcaac cttatcagtc actacgcctt
    26041 ttgatgttga cattatagct attccaagtc cacctaaaac tttcggtact tcatcttttt
    26101 ctgcatatac tcttaatccg ggtttactta ttctttttat acctgtaatg atactctttt
    26161 tatttgctga atatttcaat gttattctga ttatgccctg cttgttgtct tcaatgtatt
    26221 caatattttt tatataacct tcatcacgca atattttagc tatttctttc tttatattag
    26281 aagccggtat atcaactatc tcatgttttg ctgtactagc atttctaact ctggttaaca
    26341 tatcggcgat agcatcagtt gtctgcatat gccctacctc ctttttcaaa aaatcttcta
    26401 ccagcttgct tttttaacac ctggtatctg tcccttatac gcaagctctc taaagcacaa
    26461 tcgacatact ccatactttc ttatatatgc atgtggtctt ccgcacaatt tacatctatt
    26521 atatgctctt gtactaaact tggatttcct gctttgcttc aaaatcattg attttttcgc
    26581 cacgtatcaa tccctccttg ttaactatgg ctaaatggca tccctaatag tttcaacagc
    26641 tctttagctt cctcatcggt ccttgctgta gttacaaagg ttatatccat tcctcttatc
    26701 ttgtcaacct gatcatatac aatttccggg aagattaatt gttcttttat tccaagtgaa
    26761 taattccctc ttccgtcaaa tgaatttgca ggtacgcctc taaagtctct tacacgaggt
    26821 aaagcaatgt taaataattt atctgcaaat tcatacattc ttttcccacg taaagttacc
    26881 ttacatccga tgttcattcc ctctcttact ttaaatgctg ctactgattt ctttgcttta
    26941 gtaataaccg gcttttgtcc tattatcgca gcgagatcac ctgctgctgc atcaatagcc
    27001 ttagggtttt ccctagcatc accaacaccc atattaatta ctattttatc tagttttggc
    27061 agctgcatag gattactata tttaaatcta gcctgtaggc caggaattac ttcgcttata
    27121 tatttttcct taagtctatt cacgttttaa acctcctttc aggcatgctt tatttatcat
    27181 ctttagcttc cttgattaca tcaataactt cactacattt tttgcatacc cttgcttttt
    27241 gtccattttc cataacatct tttcctattt tagttggttt gttgcatctt ggacatatta
    27301 gcattacatt tgaactacta attgttcctt ctttttctac aatcccaccc tgctggtaca
    27361 tgcttctagg tttttgatgc tttttaacaa tatttacacc ttgtacaaca actctttttt
    27421 ccttcggtaa tactttaaga accttgccct tttttcctct acccttaccg gaaaggacat
    27481 ataccatatc tccagtttta acatgcactt tattagccaa ttcttttcgc ctccctctct
    27541 atagaacttc cggtgcgagg gataatatct tcatgaaatc tttatccctt aattctcttg
    27601 ctactggtcc aaatatacgt gtaccttttg gatttttatc atctcttata ataacagctg
    27661 cattctcatc gaattttata tatgaaccgt cagatctttt caaacccttc tttgaacgta
    27721 ctattacaca ttttactact tcaccttttt ttacaactcc gcctggtgtt gcatttttga
    27781 ctgaagcaac tactatatct cctatattag catatttcct ttttgatccg ccaagtactt
    27841 ttatgcacat catatctttt gctccagtat tatctgctga cttaagcatt gattgagtct
    27901 ggatcattag aactcctcct tccctgaatc ttcctatgga acagcttaaa ttataacctt
    27961 ttaaaaatat ttccgcatat attctcaaaa attaattatt ttgctttttc aattatttct
    28021 actacacgcc atctcttatc tttgctcaat ggacgagttt ccataactct tactttatcg
    28081 ccctctttac attcattatt ttcgtcatga gcctttaatt tatatgttct cttaactact
    28141 ttcttgtaaa gaggatgctt tacacttgtt tctatcgcta ctactactgt tttatccatt
    28201 ttatcactta ctattttgcc tacacgggtc tttcttcttc ccctatctac ctgcagtgac
    28261 actatatggt ccctcctctc atgaattacg tttggttctc aatttcaaca ttactaccct
    28321 atattatcgg gaaaacaatt tattttgctg tttttacttc tttaagttct ctttctctca
    28381 ttattgtctt taccctggct atagattttt ttacttcttt taaccgcatg ggattatcca
    28441 attgatttgt agcatgctga aaacgtaatt taaacaattc agactttaat tccgtaagct
    28501 cctgattaag ttcttgttgt gttttttctc ttatttcatt agctttcatt tgcttcacca
    28561 cccatttcct gaccgcggac gacgaatttg cattttaaag gaagcttatg agcagcaagt
    28621 cttaatgcct cacgagcatc ttcttccgat acgcccgcaa tctcaaataa tattcttccc
    28681 ggttttacta cagctaccca gtactccgga gaaccttttc cgctacccat acgggtttcg
    28741 gcaggttttt ttgtcacagg cttatctgga aatactttga tccaaacctg tccacctctt
    28801 tttataaaac gggttaacgc tattctggca gcctcaatct gattgcttgt tatccaagcc
    28861 ggttccaaag ctttaagacc atattcacca ttacttacct ggtttcccct ggttgcgacg
    28921 cctttcattc ttcctcgttg tacccttctg aattttactc tttttggcat taacatcagc
    28981 gttctcctcc ttccactttc tttacctttt tagcagggag aacttctcct ttatagatcc
    29041 atacctttac tccaattttg ccataagtag tatttgcttc agcaaaacca taatctatat
    29101 ctgcccttaa tgtctggaga ggaatagttc cttcatgata atgctcggtc cttgctattt
    29161 cagcaccgcc aagtcttcca cctaccgatg tttttattcc ttttgctcct aatttcattg
    29221 ttcttgacat agcctgcttc attgctcttc tgaaagatat tctcttttcc agttgtgatg
    29281 ctatattttc agcaactaat tgagcgtcaa gctcaggtac cttaacttca gaaatattaa
    29341 taagtatacc tttatttgtc attttttcta tttcttttct aagctcttca atccccgaac
    29401 caccttttcc aataaccagg ccgggttttg cagtatttat atttattctg attttatttg
    29461 cgaaacgttc tatttctatt cttgatattc cggaaatata aagctttttc tttaaatatt
    29521 ctcttatctt aaaatcttca ataagaaatt cactgaaatt tttatctcct gcataccatt
    29581 ttgtttccca atcttttata atacctattc ttaaaccatg gggattaact ttttgtccca
    29641 ttatccaacc tcctttgcct ttatttcctt tctttaagaa ccaaagtaat atggctggtc
    29701 ctttttctaa ttttgaatgc acttccacgt gctctaggcc ttaccctttt taatgaaggg
    29761 ccctgatttg caaagacctc agctacatac aatttatccc tgtcaagatc attattattt
    29821 accgcatttg cctcagcgga ttttaaaagc tttaaaagta tatctgaggc tgctttaggt
    29881 gtatttttca acattccata agcttcatcc agatcttttc ctcttattaa atccaatact
    29941 atttttactt ttcgtgaaga tatcctcgca aaactaaggc ttgctcttcc ttcatctttt
    30001 cctataccca gtatcctctt ctcttttttg gtgagaagga cgggcttcct ggatttacta
    30061 tgagtagaat tatatatttc taataactta tccttttgtt ttaatagctc gtctttagac
    30121 atgactttct tttccaaccg ggattcctcc tttccaaatc cttaaactat tttatttaag
    30181 ggaagttgat ttttcggtat ggtgaccgtg tcccctaaat gttctggtgg gtgcaaactc
    30241 tcccagttta tgtcctacca tttcttctgt tatataaatc ggcacatgct ttcttccatc
    30301 atagacagca atagtatgtc ctaccatttg agggaaaatt gttgatgccc ttgaccatgt
    30361 tttcaatacc tttttatcat tattagcatt catttcttct attttcttta aaagctttga
    30421 atctacaaat ggtccctttt taagtgatct actcatattt ctgattcagt cctcctttct
    30481 cagaatatat aaaagctctg attttattaa ccatttctac tcttaataat atatttatcg
    30541 gacttcttgt ttttctttct ggttttatag ccaagtgcag gcttacccca gggtgtcaca
    30601 ggagaaggtc ttccgatagg cgcttttcct tcgcctccgc catgtggatg gtctacaggg
    30661 ttcatagctg atccacgtac tttcggtctt cttcccagcc aacgtgtcct tcctgctttt
    30721 cccaaactta tattttcatg atctatattc cctaattgcc ctatagtagc tcgacaaacc
    30781 atacttacca ttcttacttc ccctgatggt aatcgtacct gcgcatagtt tccttcctta
    30841 gccattaact gcgcatagtt tccagctgac cttacaattt gtgctccttt tccaggcttt
    30901 aattcgatat tatgaattaa actacctaaa ggtatatttg caagaggaag ggcattccca
    30961 ggttttatat ctgcattaac accggattca acagtatctc caacttttaa gcccagtggg
    31021 gccaatatat atcttttctc accatcaaca taatgtaata aagcgatatt ggctgatcta
    31081 tttggatcat attctattga tgctactttt gctgctacac catctttttc tcttttaaaa
    31141 tcaataactc tatatttttg tcttgaaccg ccacccctgt aacgtacggt tatccttccg
    31201 ttagcatttc ttccaccatt tttattttta gttcccagaa gagacttttg aggcttatta
    31261 tctgttattt cctcaaatgt agaaaccgtc atatttcttc ttcctggaga ggtggggctg
    31321 tactttttca atgccatttt ttctcactcc ttcgaacgac ttaagtgaat atttcttatt
    31381 attgtattgc tccaaactct tctatacttt ctttatactt cttattaagc ccaactaact
    31441 tgccgccctt tgccaaatag gtttctgctt cagggtttat atctatttga actatggcct
    31501 ttttccaatt gggacgtttc ccttcgtgta cacccagtct ttttgtcttg cctttataat
    31561 taactgtgtt tacccatata acttttactt gaaacagctt ttcaacagct tgttttattt
    31621 ctgttttttt tgcctttgca tttactataa aggtgtactt tcctccagca gcttcaagat
    31681 tacttttttc agtaatatga ggtctgacaa ttatatcttc aaaatgcatc attatgcgta
    31741 cacctcctca actttctcta ctgcatcttt tgttataatg aacttgtcat atttcaatat
    31801 atcataaaca ttaatagtgt ttactaatgc agtctttata tttggaatat tacctgctga
    31861 tctttctacc ttttcgttct tgtcactcaa cactataaga gcactcgaat ttacatccag
    31921 attttttaat acatcaacca tctgctttgt tttaatctga tcaaacttta attcatcaag
    31981 tacaataatg ttatcagtat taactttaga agataaagct gattttaacg caattcttct
    32041 aacttttctt ggcaatttgt agctatagct tcttggtttt ggaccgaggg caatacctcc
    32101 tttaatccat tgaacagatc ttatacttcc ttgtcttgcc ctacctgtac ctttctgtct
    32161 ccatggcttt cttccaccac cactaacttc actgattgtc tttgtagatt gggttccctg
    32221 tctcttgttt gccaactgat ttacaactgc catatgaagt actaacggat ctacatcaac
    32281 tccgaatata tcgttattca gttcaatttc tcctactttt ttgccactga tgttaagcaa
    32341 atctatttta ggcatttata ttcctccctt cccaaatcat ttgctgccag ccttgacagt
    32401 ttctttaata attaataatc cacctttcgg tcctggtacg gcacctctta tcagtaatag
    32461 gtttctttcc gtatcaactc ttactacatc cagattttga actgttactt taattgctcc
    32521 catgtgtcca ggaagtttct tcctcttaaa tactcttcca ggatcagtac ctgcacccat
    32581 tgatccgact ctcctgtgat acattgaacc atgagtctca cgtccacgag caaggccaaa
    32641 tctttttata actccttgaa atccttttcc ctttgaaata cccgtaacat caactctgtc
    32701 tccctgtaca aacatatcat caacttttat ttcctgtcca ttttcaaact taccggaatc
    32761 atctactcta aattctttta aatatttctt tggacttatt ttatacttgg aaaactcacc
    32821 acgttgcggt tttataactc ttttttcctt aatatcttca aaccctagtt ttaaagaatt
    32881 atatccatcg ttttctttat ttttcttttg aacgactgta catggtcctg ctttaactac
    32941 tgtaaccggt atacataatc cttcttcatc aaataattgc gtcatcccga gttttttccc
    33001 gattataaac ttcttcatct aatttccctc caatggttat tggttcataa ttgtctatta
    33061 aatattaata attttaacgc atattatgaa cttgaccgtt tattaacact ccatttcgaa
    33121 ctaaacctgc acatattcaa cttctctgac tatccgtcta ttttccgatc cctacaattt
    33181 tatttctata tctacacccg ccggtaaatc cagcctcatc agcgcatcaa cagttttcgg
    33241 tgtcggccgg agaatatcaa ttagtctctt atgtgtacgc atttcaaact gctcgcgtgc
    33301 atctttgtat ttatgcggtg ctcttaatat tgtagttatt tccttttcag ttggaagcgg
    33361 tactggaccg gaaacactgg cacctgtcct ttttgcagtt tctactattt tctctgccga
    33421 ttgatctaaa agctggtgat caaaagcttt tagccttatt ctgattttct gattgtttgc
    33481 catatctaaa cctccttatt ctgtaacgta caacaagtgt tatactgtac actatgccgg
    33541 gtgtttccta tttgattatt ttaaaaccca ggcttaataa ccaagtttat taacctcggt
    33601 taacctgggc aatcctaata cacattcagc atgcttttaa taacatcgca acatgtacag
    33661 tgacttgtcg cccggtttct agaatcggac attctcaatg gaaattacct gctttacagc
    33721 agcaaccttc cacttcatcg tatgcctcgt cacaacagac attattctac tataatatta
    33781 tagtaatttc aagatgtttt ttaaatattt tttattttct taaatttttt tattttttta
    33841 tcgttacaat gcttgtagat cctccattta taccacttac caataatttc atattataaa
    33901 ttatataatt cataatatga aacaaggctc taagctattt gctcagagcc ttgttataaa
    33961 tccggcaggg acctactttc ccaggac
//
LOCUS       DAOSDD010000017        33222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_662_length_33222_cov_6.407966, whole genome shotgun sequence.
ACCESSION   DAOSDD010000017 DAOSDD010000000
VERSION     DAOSDD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 33222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33222
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_33222_cov_6.407966"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="PK733_02850"
     CDS             complement(<1..1778)
                     /locus_tag="PK733_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ09513.1"
                     /translation="MNYGNLARKLDEYEYIDIFENKQIGDKVKIKGKFKTPHIYYNES
                     NSYCVCSFNATYLIYDDKEIELTEIYPVTITGYYSLPDPKNEYIVTGKVNIYKKEKQL
                     ILENMKFVEPTTIKGIYSFLASGVIKGVGPKIAEKIIYGCVKNKVKVEGFGLNTLDVI
                     KNNPEKLRVIPGISNKKAAKIHESFMANIELNDIMMFFSQYDVSPVRAMKIYKTFGAF
                     SIAYVKSNPFKLLEIDGFGFDTCDKIARRLGSPPHNEDRISEGILYTLTKAAQDGHCY
                     LPENELIKKAGTILNIELPFMQAKAALKNKTGDKIDIQIDGLKYSFDRSDFENRVNET
                     EELRKNSKSTIQVPIDTITYPEIKRALANLDKRYKIKRVSSNSEIYIYKQSIYEKEQE
                     LSESILNILQNEVPNVPTKLIMKYINEYEIEHDIKLEEKQIEAITTGINNRLFVVTGP
                     AGSGKSTVISCYLYVCEKLFKGFDMGTVAQSAPTGKAAKRITEVTGIEAKTIHRLLEY
                     RPPYEYGYNRANKLPYKLIIVDEASMLDLSLAYQLFVAIDPKNTRIALIGDIEQLPSI
                     GEGNILKDMIDSNAVPVIRLDVVKRQA"
     gene            complement(1794..2510)
                     /locus_tag="PK733_02855"
     CDS             complement(1794..2510)
                     /locus_tag="PK733_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ09514.1"
                     /translation="MGKEGNQNYRVKAQEVEPIKNMKDINRVKQFFLSKEEVRNYTIF
                     VLGINVGLRAGDLLSLKMGDVFEKIDDNIYCLVDEVNILEGKTNKTRTFTLNESAKEA
                     IAFYVENLNPDYIYTTGNSNTTKKKKTNVDETRFKDIPINDYLFKSRKGSNSKLRVDS
                     LHKIVKSTMRDLGIKGNYGTHTLRKTFGYFAFTNNVKDNPRILQELQKIFNHSSEFIT
                     LSYIGITKESITNIYNSVNL"
     gene            2836..3117
                     /locus_tag="PK733_02860"
                     /pseudo
     CDS             2836..3117
                     /locus_tag="PK733_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3134..3664
                     /locus_tag="PK733_02865"
     CDS             3134..3664
                     /locus_tag="PK733_02865"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOJ09515.1"
                     /translation="MEKIDELRKRINCSYEEAKILLEKYNGDLIEAIVDFEKRNVSQF
                     RSKSGSNSVSFGTKIKQVVDKGFKTKFIIEKSGDKIINIPVNFFIIGLIIFNWFLVIA
                     LIISFVLGCIFKIRKQNGKEIDINNMVTGFGSKVKESTGDTKQNNNHNYSNNSIVKKN
                     DDSNKDYNEYTNLIIK"
     gene            complement(3968..4681)
                     /locus_tag="PK733_02870"
     CDS             complement(3968..4681)
                     /locus_tag="PK733_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ09516.1"
                     /translation="MSKQVAIITGGSNGYGKGVATYLAKDGGFDVVIVARDKARLQSV
                     AKEIGADSFSADVTKAEDWVALRKYVIGKYGKIDVLINNAGGGVAIKPLVEQTLEQIK
                     TSLDLNILSCILGCHTFVGDMMAAKSGTIINVASVCAKKGWPGFSVYSAGKGGMLIFT
                     KCLHTELQPYNIRCTTFIPAAGRTDFGKNAGQPENPSPTGFFPEDAGRAMYDIVKLPS
                     HLIVEEYIMWGMDQAVIPM"
     gene            complement(5410..7344)
                     /locus_tag="PK733_02875"
     CDS             complement(5410..7344)
                     /locus_tag="PK733_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ09517.1"
                     /translation="MKPMKRAIHLDFHTMPGIYNFGEKWDAKEFAYILKEARVDYVNA
                     FAKCNLGFTYYPTKLGIPYPTMEGDMFGELLKECHRNGIGVSAYVNVGLDHEHARLHR
                     DWCVQNKVGQVIYGDKTGNGFRAMCYNSGYREYILGIIKEVMNKYDVDGLFCDCMLLQ
                     SCYGNECIEGIKEKGLDPLNDENIVNYSKEVLMGISRDIKKLVGTDKYLYLNGVPYWA
                     AKDLDTHIEIECLPSGWSYDFFGAQVAYARCIQKKVLYMTGRFQINWGDFGGFKGKAS
                     IENDIWDGISNGTGVSIGDHMHPAENLEPEVYKVVREIYSNIEKYEEWTDGTSYISEI
                     GVLTNSDGLLSETYKGLSRMLGELKYSFCIVNEHMDISNYKVLILPDDMKVTPLLGKK
                     IEKHLNSGKGILSTGESGLNTEKTGFALKQWDFEFVGIDTSNSPYFQMTEGNRGNIGD
                     MRWAMYSPGILMKAPKDAEVFARYIRPYFNRHWDGFHGYFYTPPEKETEYAAAVKKDN
                     VYHICFKVFKAYYKSAMFSHKALIGYCLKKMLPEPLIKCSGIPSTARITITGKEDSLL
                     LHIKTTYPEVRGLMDIIEEHVTMPAGSIISVRGSYSKAYIAPQKHLLNMKINGEYTDI
                     CLPEISGYSMVVLEKKRFDI"
     gene            complement(7364..8473)
                     /locus_tag="PK733_02880"
     CDS             complement(7364..8473)
                     /locus_tag="PK733_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09518.1"
                     /translation="MLSVEKLNVGIVGIAGRPMGFISAIQNSKVARLTSVCDINYEMM
                     CKATEGIDGINKYTDYYEMIQSNTLDAVIIGTPVHLHVPQSIIALENNIHVFSEVTAA
                     TSLDECKQLVTACKHSKASYMMGENCNYMKPYMIIKEMVKDGVFGEVYYAEGEYLHNC
                     RKLIKTTPWRRKWQYGINGITYGTHSLGPIMSWFENDRIVKVSCVGSGHHYVDDDNKC
                     FEMEDTCVMLAKTIKGRLIKIRMDIVSNRPYSLEYTLQGTKGCYDSKMTGNEDDKIWI
                     EGLCNGGSWIDLNMLADKYTPELWGRYGAEASKEGHGGSDYIIMTDFIDAIVNNKPIP
                     IGIHETMDMCLPGHISQQSILCAGEWIEVPDSREW"
     gene            complement(8681..8839)
                     /locus_tag="PK733_02885"
     CDS             complement(8681..8839)
                     /locus_tag="PK733_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09519.1"
                     /translation="MLKQFYYRAKRQVSDFGITVQLTVAEVLVHDTALMDNDREWVEN
                     YLKAKYGI"
     gene            complement(9055..10821)
                     /locus_tag="PK733_02890"
     CDS             complement(9055..10821)
                     /locus_tag="PK733_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09520.1"
                     /translation="MRKGCFEKKILAFLMAIAISISLIACGNTGESAKESSNSTVQQA
                     SQSTESMPSKEPVTLKIYTNAKMSNYGIQKNPVAEEIEKATGVTMDFVANPSEETLKI
                     MLAGGDLPDIISLSATVYAKQLIEGSNIIAMDDLLKTNGKDIVSLNPKRVEFSKKYLS
                     NNAGKVYLLPYAAIASTKQIASDFSYECSAIRWDYYKELGCPEINNWDDLISVLAQMQ
                     KNHPKTQDGRKIYGLGAFTDWGTFPFTGLHSYGNNANSSDICGHTVYVDKDGNIKQTY
                     NDENSIFWQSMRDWNKAYKANIVDPDSFTQKWANFSEKGNNGQYLFEPFNWQFYGVNV
                     KLAKEENPEAGFVPIYTVFPYVSRISLTAIGDAGGSVGITSKCKAPDRAMDLINFFAT
                     YEGCRMLWSGVKGTHWDIENGKPEFKDEVFEKKSTDATYLENEGIGKYTVLAAWYGNA
                     IDPNDGVPMDLTKTTKAYKKLITPLEKSFNDFYKVDYPGQLCDKLVAEGTISFRNNIF
                     VAARGALGYGSDDIKRIDTQIVSYISKEIPKLIMAKTDTDFSNEYKTTIEELEKLGLK
                     TSVEYWTKEFNRCMSIAVEFKP"
     gene            complement(10917..11804)
                     /locus_tag="PK733_02895"
     CDS             complement(10917..11804)
                     /locus_tag="PK733_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09521.1"
                     /translation="MTGKRRIKPIKLFDFANYTLLSVFAFITLYPFYYVIIYSISDPS
                     EASKGLLFLPRGITASNYIQIMNLDGIFSAFFVSVARTVLGTILTVFSCSLFAYIVAK
                     KELCFRSLIYRFTVITLYFNAGLIPWYLLMKRLHLNNNFLLYIIPSIINAYFVILIKT
                     YIEQIPPSLEESARIDGAGYFRTFISIIFPMSLPIIATITVFSSVGQWNAWTDNFFLV
                     SDQRLQTLQLKLYNYLRQVDFSSLSMTEISSGALAKRSSPESIKATLTVIVTLPIMMV
                     YPYMQKYFIKGLFLGAIKG"
     gene            complement(11882..12820)
                     /locus_tag="PK733_02900"
     CDS             complement(11882..12820)
                     /locus_tag="PK733_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09522.1"
                     /translation="MIKPNGIEMHKKNRIMKDRLILLLMAIPFILLIFIFSYIPLFGW
                     VYAFFDYKPGIPLARTPFVGLKFFNIAFFGISGFLNSLLNSFAISLLGLLCSPLPVVL
                     AILLNEVKNTKFKKFVQTTTTLPYFISWVIVFSLFFNIFSINGALNNVLISLNVISTD
                     NPTNILGNEGATWFFQTALSLWKSTGWNAIIYVASISGIDNELYDAANVDGAGRWEII
                     KNVTLPGIIPTFIVLLLLSISNIVNNGLEQYLVFYNPLVADRIEVLDYFVYRIGILGN
                     DYALATAVGIAKTFLSLTLLLIVNVISKKTRGEYIV"
     gene            complement(13052..14911)
                     /locus_tag="PK733_02905"
     CDS             complement(13052..14911)
                     /locus_tag="PK733_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ09523.1"
                     /translation="MNIINAISSKIANSKLKIQLTISFIVTSFFVLLLSSIFTYIGVL
                     NILKNRSDQFTEKQFRQFDYNIQVFANEVDKISKLLLLDEKLQNFLEQEYEYDNSYTN
                     ILVCNDLYSKLFSIIQNYSYIDSIFIFTEDGRILGVTASTNEYLNDSNKKNGFYSSDM
                     YKLAKSSSSSLAWAGGGKSEDFNLNKIPNSRKNINIISAVRGIKPFYQNLQKATLVIN
                     IQESVITSIYSNIYDPLSSTYIVDSNGKIISDIDATRIGSNSFIFTKLDKNLDNGSFT
                     LKIDSVTKQIFYLKLTSLDWTLINEIDYKNYIKDIHTMRNILILMFFACIAISLALST
                     YWGMVFTKPIKRIINAIKEVGKGNIGITITESPKNEFGMIIRQFNEMSISIEKLIHEN
                     KMIEEMKRKQDIAVLQLQINPHFIYNTLNTIKLMAVMINAKNIVEGITIFGNILSPIF
                     KDPSPIHSIEEEFNFLKNYIKIMNLRYGEGVKIVFNIQEDLIKCKILKFIVQPLVENA
                     LYHGLAEKNYNGEIKIEIAEENENVIVTISDNGAGMTVEKLREIEEMCSGLLENSQSL
                     KGIGIRNVDHRVKLYFGVKYGLKIQSKIGSGTSAILRLPKIGEDEVQDIFQKS"
     gene            complement(14914..16515)
                     /locus_tag="PK733_02910"
     CDS             complement(14914..16515)
                     /locus_tag="PK733_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09524.1"
                     /translation="MYKVLIVDDEILVRLALKNSINWNKYGMTVIADVQNGHAAWEIY
                     KKEKPDLIITDIKMPIIDGMELIKKIREKDKETVVIILSCLEDFELLRQAVSLNISGY
                     ILKLTMSNEEIENVLSKAQDELKGIHEGKPTLTLDNNLIKEKMIKELIFYNNCSINEF
                     NQSVIGLKLRIKPNNLLMCLIEIDHFEQVRKGYSYENGQVLSQSILNILDEISNQYER
                     IEALRVDDSHYILLFSFHDISSEQSIQEELYTILGHIKKVLKTYFNSTATFGISGLRN
                     GFASLKDLYTECLDILKQKFYLGLDTYITNSSKSFVRKAYENNKESLKEICGGVYMFN
                     ELIRNGINSRIESFIQSEDISSKEQVQNLFVEWIYWLYVSLNLNGEDIDVLLGSCLEQ
                     LHYSETFEEMIEVFKEYIDGVNNIAGKKKALSREVAKATRFIKVHYPEDITLQQVAEH
                     VNLSPNYLSGLLKREIGLSFVEYLNYIRITKAKELLSNTDLKSYEIAEKVGFTDDSYF
                     SRSFMKQTGIRPNKFRRQSMQVLGE"
     gene            complement(16611..16829)
                     /locus_tag="PK733_02915"
     CDS             complement(16611..16829)
                     /locus_tag="PK733_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09525.1"
                     /translation="MISQLEFVRMVACPEISDLDAQVLERGYLCLNAELGVLTLILHI
                     FEIMVLLTIAWNWSKKQVNMAYIPTTTW"
     gene            complement(16848..17120)
                     /locus_tag="PK733_02920"
                     /pseudo
     CDS             complement(16848..17120)
                     /locus_tag="PK733_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(17086..17220)
                     /locus_tag="PK733_02925"
     CDS             complement(17086..17220)
                     /locus_tag="PK733_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09526.1"
                     /translation="MSSDTVIAVAKANNGPAKVYCPDAPELASQYTRNAATGTWNEEM
                     "
     gene            complement(17210..17461)
                     /locus_tag="PK733_02930"
     CDS             complement(17210..17461)
                     /locus_tag="PK733_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09527.1"
                     /translation="MTKECEAVDITSKGFACYSTFFELPEFERTAGADDFSCWWELIG
                     NDFDRWVSFGMMEVYPMVGKNNSSGNTKGNKGNIGVYEQ"
     gene            complement(17538..17714)
                     /locus_tag="PK733_02935"
     CDS             complement(17538..17714)
                     /locus_tag="PK733_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09528.1"
                     /translation="MNNRVNYCHLKDWIQYGGGSWVAAGVGDGTMDWKPLLEKMSFKG
                     VYLLEYEPTYDVED"
     gene            complement(17925..18326)
                     /locus_tag="PK733_02940"
     CDS             complement(17925..18326)
                     /locus_tag="PK733_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09529.1"
                     /translation="MIVGVGEWGFRELPFEEHCRIAAKFGFKYMKLGIGGDFIGRLRG
                     DMTEAELNNTLSCIKDYGIKTPFMCLENDFTKGNANEVWKTVERICMEAKLSRAFVVT
                     YLRLFAGFVPAEKMSSEKRNNMIMAFKAVDH"
     gene            complement(18397..18639)
                     /locus_tag="PK733_02945"
     CDS             complement(18397..18639)
                     /locus_tag="PK733_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HOJ09530.1"
                     /translation="MISRKNFLFCNAPDRATASAMIYSMIETARANNLKPFEYLAYLL
                     KTLPNVDAKDQSMLDSLMPWSVELPESCRLNDMSLQ"
     gene            complement(19035..19274)
                     /locus_tag="PK733_02950"
     CDS             complement(19035..19274)
                     /locus_tag="PK733_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09531.1"
                     /translation="MVYDLNFNVYADFTSFGAENVKSIAESIKRKYIDTLKNDYTRLY
                     WTDTETKLFSHDMLTFVRPMVSGTGYKYGWLFVLI"
     gene            complement(19305..19640)
                     /locus_tag="PK733_02955"
     CDS             complement(19305..19640)
                     /locus_tag="PK733_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09532.1"
                     /translation="MRKRILKWLHRSIVKLIVILVCGTVIPELLLAGVSSAVIQDIMY
                     KNFYNNIHTNLRIASDAISVVKDKVYSVANNMVFNTILYKLLTNDYSSDVFTKYKDLD
                     KVSKTLFSS"
     gene            complement(19717..21120)
                     /locus_tag="PK733_02960"
     CDS             complement(19717..21120)
                     /locus_tag="PK733_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ09533.1"
                     /translation="MYYDIIIIGGGSSGVGACLAASDTNPDASILLVERETILGGTST
                     VGGVHNWEPGVSTGRYHVEIAKRLLESGKGCVAKDVWHMTPEKPMGISEPCSDSYERT
                     RRRAGVDSWDQRRFHFEPLAMDRVMKDMISDRKNVTLLLNTEFVRSRTEKRKIKELVL
                     KQDSREFTVSGQVYIDASADIYLARDAGCGWNLGREASSEYGEPSAPEEHENYLNACS
                     LIFRIQKKDESDVDPIPSPYDSIDVSDWAKENLPNNRPVSFVTQYPNGDYCINMLPTI
                     EGRVLFDMEYGKGYELCKARVYRYFKWLQEEKGFAGYRLISFAPRIGIRETYRLKARH
                     VLTENEVRMPFGEQPLSDEIIVYADHAIDVHGKKHSSNDDQNLKYPYGIPYSCLLPVE
                     RDNLLVSCRGSGFTHIVGASCRLSRTMMAIGEAAGTAAAICLKDGCNPPEVDVSKVCG
                     QMDMEYVKNNICELFGI"
     gene            21637..22593
                     /locus_tag="PK733_02965"
     CDS             21637..22593
                     /locus_tag="PK733_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ09534.1"
                     /translation="MTQGTLLKKFFETAFVDKSTELGIKDPHDKAIFDGCKARSFCKQ
                     RYKPDKSCRCSSNSIKPFKCSMCTDPEATRGYDHNLDEFLMGYGLHFAVTPLVNSPSK
                     CLLLTFFVTGADVHDSKVFPHLLIYLYHDMKVDFNYVILDSGYDAWYIYWWISLLGGK
                     PIIAINPRNSQTPTPENEYGLPVCHAGHAYYYWGIDRKKRRYKWRCPLKASKKLSKKL
                     KCDKPCCNSSYGKTVYTHIEDDIRWFTQFPRGSQLWKDLYSRRARIEPVIGYSVLNYG
                     LKTPLFQGTKNFFVRTALVCMIQHAKAIASKLLSIEYQKTAA"
     gene            complement(23653..24579)
                     /locus_tag="PK733_02970"
     CDS             complement(23653..24579)
                     /locus_tag="PK733_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09535.1"
                     /translation="MEPVKEYKVRSFRADDETFEKFKTLANEEFGNQGQCLASLISLY
                     ETEKSKAVLVERKIEIESFQTYLNKITEMFLMSLQLNEDAEERIRSEFEHLLSSKDRI
                     ITDLQNKETELTAANELYKETAKEAEQENYRLLNQISELEKQISKQNREYEATLADKD
                     NLNKALTDSCNEKEGKIKELKEKLDNTLSEISKLNEIKTHNNQLMTENKQLMAEIEKQ
                     KEHSILEKEKAILAAEKTHQKELKETQLQHQKDYKELMEQIDKLQEQRNNTINEVTEL
                     KLQNNELQGKIKEFERIIADLEEKLGRQKVEK"
     gene            complement(24758..24943)
                     /locus_tag="PK733_02975"
     CDS             complement(24758..24943)
                     /locus_tag="PK733_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09536.1"
                     /translation="MKLYGNSRKVYLVHKMGEENYKLCKVLNEYSSVDEAHDDLTKLL
                     TDQKTEGQLLREFGKKE"
     gene            complement(25900..26361)
                     /locus_tag="PK733_02980"
     CDS             complement(25900..26361)
                     /locus_tag="PK733_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOJ09537.1"
                     /translation="MAKRISIYSVRLIKEKTGLYELEYVVIRNSQIANDVIKKVLDIN
                     NSTVEKFGIITLTVKNKIAGVHIISVGNLNSSIVHPREIFQQAILNNASAIILFHNHP
                     SGDPTESTEDIEITKRLIEAGDILGIKVIDHIIVGEDNKYISFKAKGICNI"
     gene            complement(26411..26824)
                     /locus_tag="PK733_02985"
     CDS             complement(26411..26824)
                     /locus_tag="PK733_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09538.1"
                     /translation="MGTKLAVATTIEGNVTSEGLAALNDCEDLTTALARELASGISAN
                     VEDVSAIYKKMAILNTKKQVQTKDKNISVKDMQLGIIEKEQNDITTNIIDDLEIENAK
                     LKLLFKTSKPKNGKSRKKIMEGQLDLFSYFLNKVA"
     gene            complement(27099..28925)
                     /locus_tag="PK733_02990"
     CDS             complement(27099..28925)
                     /locus_tag="PK733_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOJ09539.1"
                     /translation="MRNPIDVLNSLRSKSKNPDYHYERLYRNLYNAEFYTEAYQNIYA
                     KPGNMTAGTDGKTVDGMSMERIEALIASMKDHSYQPYPARRTYIKKKNGKLRPLGIPS
                     FNDKLVQEIIRMILESIYEPTFSNRSHGFRPDRSCHTALQQLKANFTAVKWFIEGDIT
                     SFFDNINHAVLVGILRRRIHDEYFISLIWKFLKAGYIEDWTFYRTYSGTPQGSIISPI
                     LSNIYLNEFDRFMERYMESFKAEKIRAKYKPYWQLHEKIRWLKDGKILPEMWENMGND
                     NKKEYLKNIRYMTNEKIKMPTKDPMDSNYRRLQYLRYADDWICGVIGSKEDAEMIKAD
                     VKKYLSEELKLELSEEKTLITNAHDKARFLGFDICTSQSQQTIEDKNGNRARYQTGRI
                     KLYVPREKWQKKLTDYSALKIKYQNGKEIFVPCERTYLINSDDLEILNQYNAEIRGIY
                     NYYRIADNASVLGDFYYVMKYSMFKTFAAKYKTRISKIRKKYGYKRFGVKYPSKSGKA
                     VAYLYDGGFRHDGSIIKMPDVDVIPLVHRNLNRTSLISRLKAQKCEWCGAEDVPIEMH
                     HVRKIKDLKDKTGWEIVMIGRKRKTMALCPTCHDKLHAGKLD"
     gene            complement(29553..32018)
                     /locus_tag="PK733_02995"
     CDS             complement(29553..32018)
                     /locus_tag="PK733_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOJ09540.1"
                     /translation="MINGLFKVEYTNYDKDIYVTNYAECIVYDSRTNQLVLMYYGGYT
                     ETVRAMADAICSGGGIKVHTETHIMNFNSVAKAYKKNIKKDDPYTKCLLLRKDDENYV
                     RNDNEEENILRKSYLFIKDKNKKQLFKEINKRTSIPMLDEYVDYVIEKMQEEEILYKL
                     ECLSNTNLLDAYCMIRDDDTIQRIVETGLKEGHLKISDIKPKEKGFLGISDITTYLSK
                     FGQEIAEKIKKSFVPLFDPTKEKISKEVEEIDNYLYQTRGIRLYSAQKIIAESMKKKL
                     EKDKCVVLCAECGSGKTVIASTALYSLYKNKKEKHFGIILGPGHLGEKWVREIEWTIP
                     NSKAFIAQTITELNKAYEYYTENRKTVYVFISKERARDGYMKKPAVIWSNAKKCFICP
                     DCFSKQEMELTEDDVKYMVDADCFYYRNENSANRKCKNCNSLLWTAVNPSDRRYNKWA
                     KVSPYGWVYRPMAKHYFKEVEDEKTLEQLSDIIAKTGYIPTKSAYRKYGLSTYMKKKM
                     EHVVDSCILDELHLYKGDTGQGIAAGELAKMSKKVIAMTATLINGYSSSIFYILFRCF
                     PSLMVKEDYEYYDQRAFNKEYGVFQEEYKLKEARYNAGSRAVKRKLREKLLPGVSPLV
                     FTRYLLDNAVFLSLDDMTDSMPEYEEIPIIMKMDEEVANEYERIEGELKSILKSERNI
                     SHKILGNYLIMLTLYPDMPYGMKPIYSPEDGSELVVPKNIGSIDNLYAKELKVLEIAE
                     EKISKGEKLLVYTNWVNTDTQKKLQKILTEKDYRTKILSSSIQASKREEWLMKNANEI
                     DVLVTNPSLVETGCASVRTQIAA"
     gene            complement(32122..32703)
                     /locus_tag="PK733_03000"
     CDS             complement(32122..32703)
                     /locus_tag="PK733_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOJ09541.1"
                     /translation="MSLPKKEDKQYTYADYLTWPEDKRWEIIDGVPYMQAAPTWQHQA
                     ISRELLTQFNNYLKDKPCQIFASPFDLRLSDVDETDEGTTNVYQPDLVVVCDETKLKG
                     TGYHGIPTLIIEITSPSTVRNDKIKKFNKYEEVGVREYWIIEPEYRIVTVFTLQENKR
                     YGRLKHYTEADKVNVSVFPDLEIDLSTVFPATE"
     gene            complement(32746..33117)
                     /locus_tag="PK733_03005"
     CDS             complement(32746..33117)
                     /locus_tag="PK733_03005"
                     /inference="COORDINATES: protein motif:HMM:NF022987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3232 domain-containing protein"
                     /protein_id="HOJ09542.1"
                     /translation="MLREKFNALVNKLISMNEDELASDIEDIIDSCGKYIDAVNSMEA
                     AITCARFRLEPEAYREYIVRLDVNRRRLHDMVIVNIKLLNRLCKMAEIESIYTGDLES
                     RIELAEFAMQTVNEMFNTRKL"
BASE COUNT         9632 a         6732 c         4926 g        11932 t
ORIGIN      
        1 gcttgccgtt ttactacatc tagtcgaata acaggaacgg cattactatc tatcatgtct
       61 tttaatatat tgccttctcc tattgatggt aactgttcta tatcaccaat caatgctatt
      121 cttgtattct taggatcaat ggccacaaat agttgatagg ctaatgacaa atctagcata
      181 gatgcttcat ctactattat taatttataa ggcaatttat tagcacgatt atatccatat
      241 tcgtaaggcg gcctgtattc taataaacga tgaatggtct ttgcttctat tcctgttacc
      301 tctgtaatcc tctttgctgc cttgcctgta ggtgcagact gtgccacagt acccatatca
      361 aaacctttaa ataacttttc acatacatac agataacaac ttatcactgt acttttacct
      421 gaaccggccg gacctgttac tacaaatagt ctattgttaa tacctgttgt tatcgcctca
      481 atctgttttt cttctaattt tatatcatgt tctatttcgt actcattaat atatttcatt
      541 attaattttg ttggtacatt agggacttca ttttgtaaaa tatttaatat gctttctgat
      601 aattcttgtt ctttttcata tatagactgc ttatagatat atatttcgct attgctgcta
      661 actcttttaa tcttatacct tttatctaag tttgctaaag ctcttttgat ttcaggatat
      721 gtaatagtat caataggtac ttgtatggtc gattttgagt tttttcttaa ttcttctgtt
      781 tcattaaccc tgttttcaaa atcactacgg tcaaaactat attttaggcc gtctatttgt
      841 atatctattt tatcgccagt cttgtttttt aaagcagctt tggcttgcat aaacggtaat
      901 tctatgttta gtattgtgcc ggcttttttt atcaattcat tctcaggtaa atagcaatgc
      961 ccatcttgtg ctgcctttgt aagagtgtat agtatacctt cactaatacg gtcctcattg
     1021 tgaggcggac ttcctaatct ccttgctatc ttgtcgcatg tatcgaaacc aaaaccgtct
     1081 atctctaata gtttaaatgg attagattta acataagcta tagaaaatgc tccaaaggtc
     1141 ttatatatct tcattgcacg aaccggacta acatcgtatt gagagaaaaa catcattata
     1201 tcgtttaatt ctatattggc cataaaggat tcgtgtattt ttgcagcctt tttatttgat
     1261 atgccaggta ttacccttag tttttcagga ttattcttta taacatctaa tgtgtttaat
     1321 ccaaaccctt ccacttttac tttgtttttc acacatccat agataatctt ttcggcaatc
     1381 tttgggccaa ctccttttat aactccagat gccaaaaaag aataaatgcc ttttatcgtt
     1441 gtaggctcaa cgaatttcat attttccaaa attaattgct tttctttttt ataaatgtta
     1501 accttgcctg ttactatata ttcgttttta ggatcaggaa gactgtaata gcctgttata
     1561 gttactggat atatttcggt caactcaatt tctttatcat cgtaaattaa atatgttgca
     1621 ttaaaactac atacacaata gctattgctt tcattataat atatatgtgg tgttttaaac
     1681 ttacctttaa tttttacctt atcgccgatt tgcttgtttt caaatatatc aatatattca
     1741 tattcgtcta actttcttgc taagttccca taattcaata ctaatacctc cttttacaga
     1801 ttaacactat tataaatatt agttattgat tcttttgtta tgcctatata tgaaagagtt
     1861 ataaactctg aactatgatt aaatatcttt tgtagttctt gcaatatcct tggattatct
     1921 tttacattgt ttgtaaacgc aaaataacca aaggtttttc taagggtatg agtgccgtaa
     1981 ttacctttta tgcctagatc tctcatggtg ctttttacaa tcttgtgtaa actatctact
     2041 ctgagcttac tatttgaacc ttttctactt ttgaacaaat agtcattaat tggtatatct
     2101 ttaaaccttg tctcatctac attcgttttt ttcttttttg tagtatttga atttccggta
     2161 gtgtaaatat aatccggatt taagttttct acataaaatg ctattgcttc tttagctgat
     2221 tcatttaatg tgaatgttct tgttttattt gtcttgcctt ccaatatatt gacttcgtct
     2281 acaaggcagt aaatattatc atcaatcttc tcaaatacat ctcccatttt taacgataat
     2341 aaatcaccgg cccttaaccc tacatttatt cccaaaacaa atattgtata atttctaact
     2401 tcttctttgc ttaaaaaaaa ctgtttcact ctattaatat ctttcatatt tttaataggc
     2461 tcaacctctt gagccttaac tctgtaattt tggtttcctt cttttcccat gaacatcctc
     2521 caaaatatat aactcaattt ctttttaaat accaacttat actaatatta tatcataact
     2581 tggtattatt gacaacaact tatttgcgtt ttattattct taaatctctg taaccattat
     2641 caatactatg tttggagact ttttctgtac atatatgaat accaacttag tatataagtt
     2701 ggcattggta gacaaagtgg atttcttttt ggcttagaat tattttaatg gtaggatctt
     2761 aaacttgcaa cttataaagt ctatttatat aattttttgc ccatattagt ataatcctca
     2821 tcattctata ggtttggtag aaactatgag gaaataaaac aggaattagc ttacttccaa
     2881 agtatgggta taaaggtaag aataccagat cttcccgttt taaatgttga agatgagact
     2941 ctttcttcct tattaaataa tcttattatt gaattacttt catacatagc tcaaaaggaa
     3001 catgaaaaaa tacaagaaag ggttaaagaa ggacttgaag ctgccaagaa aaaaggcata
     3061 aaacttagaa gacccgaaag gaagctccct gataattttg agaagtatta taaatgatag
     3121 tatgagtatt tcattggaaa aaattgatga gttaaggaaa agaataaatt gcagttatga
     3181 ggaggcaaaa attctccttg aaaaatataa tggggattta attgaagcaa ttgttgactt
     3241 tgaaaaaaga aacgtttcac aattcagaag taaatcaggt agtaattccg taagcttcgg
     3301 tactaaaata aagcaggtgg ttgacaaagg ttttaaaaca aaatttatta ttgaaaaatc
     3361 cggagataaa ataataaata taccagtaaa cttttttata ataggattaa taatatttaa
     3421 ctggttctta gttatagcac tcattatttc atttgttctg ggatgcatat ttaaaatccg
     3481 taaacaaaac ggtaaagaga tagacattaa taatatggtt accgggtttg gatccaaggt
     3541 aaaggaatcc acaggagaca caaaacaaaa taataatcat aattattcaa ataactctat
     3601 tgtaaaaaaa aatgatgata gcaataaaga ttataatgaa tacacaaatt tgataatcaa
     3661 gtagaagcca gataatcagt ttagtgttga tataaaatct ctcctccaga attttatttt
     3721 tataaatacg tttcaggaaa gagagatttt aattgtctat acacaagatt ttttacattc
     3781 tctatctgca agctcatttc taaagttacg ctctttcaat gaggccattg ctctttcatc
     3841 gtatggatat gcctctaacg caaacaccat aatcttcttg tcttttaatc tattaaaatg
     3901 tttttaagaa aggaaatgcc agcaatacct atagcatata ttccacctcc taaaaacact
     3961 ttatttttta cattggtatg acagcctgat ccatacccca catgatatat tcctcaacaa
     4021 tcagatggct gggcagtttt acgatatcat acatcgcccg tccagcgtct tcgggaaaaa
     4081 atccggtggg actggggttt tcgggctgac cagcgttttt accgaagtct gtacgtcctg
     4141 cggcagggat aaatgttgtg cagcggatat tatagggctg cagttccgta tgtaggcact
     4201 ttgtaaaaat gagcatccct cccttgcctg ctgaatagac cgaaaatcct ggccatccct
     4261 tctttgcgca gaccgaagca acattaatga tagttccgga ctttgcagcc atcatatccc
     4321 caacaaaggt atggcaaccc agtatgcagc ttaaaatatt caaatctagc gatgttttga
     4381 tctgttccag agtctgttct acaagaggtt ttattgccac gcctccacct gcgttgttaa
     4441 tgaggacatc tattttgcca tacttgccga tcacatattt gcgaagcgca acccagtctt
     4501 ccgcttttgt cacatctgcc gaaaaagaat cagctccgat ttcttttgcc acactttgca
     4561 gcctggcttt gtccctggcc acgatgacaa catcaaaacc gccgtccttt gccaggtatg
     4621 ttgctactcc cttgccatag ccattggagc ctcccgttat aattgcaacc tgcttactca
     4681 tatgaatatt acctccaaag catattaagt tctctcggaa accctttccg aggctataaa
     4741 tatatacttt caatttgttt acataacaac attccctcca ttcccatcca catacttcct
     4801 ttcagcgtta ttactacgtt atagctatta atatggagaa atatgaagtt acctacacta
     4861 atttaagcct aagttaaaat aatatgactt gacaaatgga aaaccgcccc aaaacggaat
     4921 tatatggtat atggtgccat taattcttga aacgaggtga ttttatgcag ttgtcaaaaa
     4981 cttcccatga gctttaccaa agctaccttt tatctttcac caactatagt tgctaggagc
     5041 gcactatatt tggttcatac aaattgaaat tgtagtgccg tttcattcca catgaattga
     5101 acaatctgat tgtattttgc atgaatccca gacaggaatg tcaaagcatc caatctatgg
     5161 aaaactgtat ctgataagtt atttgctgta tgttcctttc atctgacctg atgatccagc
     5221 ttaatcgcag tttttgctct acaatttcta gttaattgct cttatactca cactttaatt
     5281 cagtaacact gataacacta attattctcc ctcttttgct ttttgttaat attatccctt
     5341 tatttatcag aaccttattt gtatgctgcc tttaaaagca agtttccgcc tttcactgct
     5401 aaaaaaacct catatatcaa atcgtttctt ttcaagaaca accatactgt aaccagatat
     5461 ctccggaaga catatatccg tatactcacc attaatcttc atatttagta gatgtttttg
     5521 tggtgcaata tatgcctttg aataacttcc cctaacactt ataatagatc cagcaggcat
     5581 agtaacatgt tcctcaatta tatccataag tcctctaacc tcaggatatg ttgtttttat
     5641 atgtagcaac agactatctt cctttccggt gatagtaata cgtgctgtag aaggtatgcc
     5701 actgcattta attagaggct caggtagcat cttcttcaga caatacccga taagtgcttt
     5761 atgagaaaac attgcacttt tgtaataagc cttaaatact ttgaaacaaa tatggtaaac
     5821 attatctttt tttactgccg ccgcatattc ggtttccttt tcgggtggtg tgtagaaata
     5881 gccgtgaaag ccatcccaat gccggttaaa atatggcctt atataccttg caaatacttc
     5941 tgcatcttta ggcgctttca tcaggatacc agggctgtac attgcccatc gcatatctcc
     6001 aatattacct ctattgcctt cagtcatttg gaagtacggg gaatttgaag tgtctattcc
     6061 aacaaattca aaatcccatt gttttagtgc aaaccctgtt ttttctgtat tgagtccgga
     6121 ctcccctgta cttagtatcc ctttaccact attaaggtgc ttttcaatct ttttacccag
     6181 taacggtgtt actttcatat catcaggaag tatgagtact ttataattag aaatatccat
     6241 atgttcattt acaatgcaaa agctatactt caattcacct aacatgcggg aaaggccctt
     6301 ataagtttca cttaataatc catccgaatt ggtgagtaca cctatttctg agatgtaact
     6361 tgttccatcc gtccattctt catacttttc aatgttgctg taaatctcac gtactacctt
     6421 gtaaacttca ggttcaagat tttcagccgg gtgcatatga tctcctatgg atacacctgt
     6481 tccattactt attccatccc atatatcatt ctcaatagaa gctttccctt tgaatcctcc
     6541 aaaatcgccc cagttaatct gaaaccttcc ggtcatataa agcacctttt tctggataca
     6601 tctagcataa gccacctgtg ccccaaaaaa atcgtaactc cacccgcttg gcaggcactc
     6661 aatttcgata tgagtatcca gatcctttgc agcccagtag ggcacaccat taagataaag
     6721 atatttatca gttcctacca acttctttat atcacggctt atacccataa ggacttcttt
     6781 tgaataattt acaatgtttt catcattcag aggatctagc cctttttctt taattccttc
     6841 tatacactca tttccgtaac aactctgtag caacatacaa tcacagaata aaccgtcaac
     6901 atcatatttg ttcataactt ctttaataat accaagtatg tactcacggt atccgctatt
     6961 ataacacatc gcgcgaaagc cgttccctgt tttgtcccca taaataacct gcccgacctt
     7021 attttgtacg caccaatctc tgtgaagcct tgcgtgctca tgatccaatc ctacatttac
     7081 gtatgctgaa acaccgatac cattcctatg acattcttta agtaactcac caaacatgtc
     7141 accctccatt gtggggtaag gtattcctaa tttggtggga tagtaagtaa atccaaggtt
     7201 acatttggca aacgcattaa cataatctac tctcgcctct tttaatatat atgcaaactc
     7261 ttttgcatcc catttctctc caaagttgta tataccaggc attgtatgga aatctaaatg
     7321 tatagctctt ttcattggtt tcataaatgt ttttactcct cttctaccat tctcttgaat
     7381 caggtacttc tatccattct ccagcacaca aaatggactg ctgacttata tgtccaggta
     7441 aacacatatc catagtctcg tgtataccta tcggtatcgg tttattattt actatggcat
     7501 caataaagtc agtcattata atataatccg atcccccatg gccttccttg cttgcttcag
     7561 ccccatatct tccccataat tcaggagtgt atttatcggc aagcatattc aaatctatcc
     7621 aacttcctcc attacaaagc ccttcaatcc aaattttatc gtcttcgttt cctgtcattt
     7681 ttgaatcata acagccttta gtaccctgta aagtatattc cagactgtat ggcctattcg
     7741 aaacaatatc cattcttatt tttatcaatc tgccctttat ggtctttgca agcattacac
     7801 aggtatcttc catctcaaag catttgttat catcgtccac gtagtgatgt ccacttccta
     7861 cacacgatac ctttacgatt ctatcgttct caaaccagga cattataggg cccaagctgt
     7921 gagttccata tgtaattccg ttaatcccat attgccattt tcttctccat ggagtagtct
     7981 tgataagctt cctgcaatta tgcagatact ccccttccgc ataatacact tcaccgaaga
     8041 ctccgtcttt taccatttct tttattatca tgtaaggttt catgtaatta cagttttcac
     8101 ccatcatata gcttgctttt gaatgtttac atgctgtaac aagctgttta cattcgtcca
     8161 atgatgtcgc tgcagttact tcactgaata catgaatatt attttccaat gcaattattg
     8221 attggggtac atgaaggtgc acaggtgtac cgataattac agcatctagt gtattgcttt
     8281 gaatcatctc ataataatct gtatatttat tgattccatc tataccttca gttgctttac
     8341 acatcatttc atagtttatg tcacatactg aagttagtct tgccactttg ctgttttgaa
     8401 tggcagaaat gaaccccata ggcctcccag caatacctac tataccaaca ttaagttttt
     8461 caacactcag cattttttag tcctccctta ataaaataat ccgcacgttg gttgtatcta
     8521 tatcaaactg tacctattat gaggatgctt aattaataag cgccctcact atatgaatcg
     8581 cactaaaaat ttacatctta tgcttattga acgcacaaat gtatagtcta acgtacaatc
     8641 caaattagta atgtaaaggg attcgtgcgg tatctataat ttaaattcca tattttgcct
     8701 tcaagtagtt ctcaacccat tcacgatcgt tatccataag ggctgtatca tgcacaagaa
     8761 cctctgctac tgtcagttgt actgttatcc caaaatccga tacctgcctt tttgccctat
     8821 aatagaattg ctttagcatg acttccaact ggtaacacta ttttccaacc atacaagagc
     8881 tccctcaaat atatttagaa gctcttgtat caatttttga ttatataatc acagaaatga
     8941 ttttacatcg gacgaattag atttctgcga tgctgttatg gaaattgaag cgcacaaaac
     9001 ttgggatgta aaatcttttg tgcgctcaat atagtaaaag caaccagctt gtattcaagg
     9061 tttaaattca acagctatgg acatacatct attaaattct ttagtccaat actccaccga
     9121 tgtttttagt cccagcttct ctaattcttc aattgtagtt ttatattcat tactaaagtc
     9181 tgtatcggtt tttgccataa tcaattttgg tatctcctta cttatgtaag aaactatttg
     9241 tgtgtcgatt ctcttgatat catcgcttcc gtaacctaaa gcaccccttg cagcgacaaa
     9301 aatgttgtta cggaaactaa tcgttccttc tgcgaccagt ttatcgcata gttgccctgg
     9361 gtaatccact ttgtaaaaat cattaaatga tttctccaac ggagttatga gctttttgta
     9421 agcttttgtt gttttagtca aatccatagg cacaccatca tttgggtcta tagcatttcc
     9481 ataccaagca gcgagtactg tatatttacc aattccctcg ttctcaagat aagttgcatc
     9541 cgtactcttc ttttcaaaaa cttcatcctt aaattccggt tttccatttt cgatatccca
     9601 atgcgtacct ttcacgccac tccataacat cctacatccc tcatatgttg caaagaaatt
     9661 gattaaatcc atcgccctat caggggcttt acatttgctg gtaattccta cagaaccccc
     9721 agcatctccg atagcagtta aacttattct cgaaacatat gggaatacag tatatatcgg
     9781 tacaaacccg gcttcggggt tttcctcttt tgctaatttt acattgactc catagaattg
     9841 ccaattaaat ggttcaaata agtattgtcc attgttaccc ttttcactaa aatttgccca
     9901 cttctgtgta aatgaatcgg gatcgacaat atttgcttta tatgctttgt tccaatcgcg
     9961 cattgattgc caaaaaatac tgttttcatc attataagtt tgcttaatgt tcccatcctt
    10021 atcaacgtat acagtatgac cgcatatatc actactatta gcattattac catatgaatg
    10081 caaaccagta aacgggaacg taccccaatc tgtaaatgct cccagtccat atattttcct
    10141 tccgtcctga gttttaggat ggtttttctg catttgcgcc aaaacactta ttaaatcatc
    10201 ccaattgttt atttcagggc atcccaattc cttgtagtaa tcccacctta ttgcagaaca
    10261 ttcatacgaa aaatctgagg caatctgttt cgtactcgca attgcagcat aaggaagaag
    10321 atatactttt cctgcattat tactcaaata cttttttgaa aattcaaccc tcttgggatt
    10381 aagtgagact atatccttgc cattagtttt caacagatca tccatcgcta ttatattgct
    10441 tccttcaata agctgcttag catacactgt tgcactaagc gatattatgt caggcaaatc
    10501 tccgcctgcc aacataattt ttaatgtttc ctcagaaggg ttggcgacaa aatccatagt
    10561 tacgcctgta gctttttcaa tttcctcagc gacaggattt ttttgaatac cataattact
    10621 cattttcgca tttgtgtaaa ttttaagagt aacaggttct ttggatggca tactttcagt
    10681 gctctggctg gcttgttgca cagttgaatt ggaactctct ttagcagatt cccccgtatt
    10741 tccgcatgcg attagtgaaa tcgaaatcgc aatagccatt aaaaatgcta gaatcttttt
    10801 ttcaaaacat ccttttctca ttgatttccc tcctactttc attttatttt gattaacaga
    10861 ttaacagatc acgattagtc tcgggatccg ttgaatgcaa aaaaacaata tcacgctcac
    10921 cccttgatgg cgccaagaaa taagccttta ataaaatact tttgcatata tggatatacc
    10981 atcataatcg gaagcgtgac aattacagta agtgtagctt taatagattc aggagatgac
    11041 ctttttgcca gtgcaccgct agagatttct gtcattgata aagaagaaaa atcgacttga
    11101 cgaagataat tataaagctt taattgcaat gtctgtagcc gttgatcaga aacaaggaaa
    11161 aagttatccg tccatgcatt ccactggcca actgaagaaa aaactgttat tgtagctata
    11221 attggtaacg acataggaaa aattatacta ataaaagttc taaaatatcc ggccccatca
    11281 atcctagcag attcctcaag cgaaggaggt atttgttcta tatatgtttt tataagtatt
    11341 acaaaatatg cattaataat acttggaata atatataaaa gaaaattgtt atttaagtgt
    11401 aaacgtttca ttagtagata ccaaggaatc agccctgcat taaagtataa agtgataaca
    11461 gtaaatctgt atatcaaact tctaaaacat aactcctttt tggcaactat atacgcaaat
    11521 aagctacatg aaaatacagt taaaatcgta cccagaactg ttcttgctac tgaaacaaaa
    11581 aatgccgaaa aaattccatc caaattcata atttgaatat agtttgaggc agtaattccc
    11641 cgaggtaaaa ataatagtcc ctttgatgct tcactcggat cgcttataga atatattata
    11701 acataataga aaggatataa tgttataaag gcaaacacag ataataaagt atagtttgca
    11761 aagtcaaaca atttaatagg cttaatacgt ctttttcctg tcatctttta cctccgctgt
    11821 tcttaattgg ataaggaatt attaaatatt gaaataacag tttaaaaaat gcaatagcaa
    11881 atcatacaat atactctccc cgcgtttttt tggaaataac atttacaatt aaaagtaatg
    11941 tcaagcttaa gaacgtcttt gcaataccaa cagcagttgc aagtgcataa tcatttccca
    12001 agataccaat acggtaaaca aagtagtcca aaacttctat cctatcagca acaagggggt
    12061 tataaaatac gagatattgc tctaatccat tattaactat attagatata ctaagaagca
    12121 ataatacgat aaatgtcgga ataataccgg gtagggtaac atttttaata atttcccacc
    12181 ttccagctcc atctacgtta gctgcatcgt atagttcatt atctattcca gatatggaag
    12241 ctacatatat aatagcattc caccctgtac ttttccataa cgacaaggct gtttggaaaa
    12301 accatgttgc accctcattg ccaagaatgt ttgtcggatt gtcggtacta ataacattca
    12361 aactgattag cacattgttt agcgcaccat ttatcgagaa tatattgaaa aacaatgaaa
    12421 atacaataac ccagcttata aaataaggta atgtagttgt tgtctgaaca aattttttga
    12481 acttggtatt ttttacttcg tttaaaagta ttgctaaaac aacaggtaac ggagaacata
    12541 atagacctaa caaactaata gcaaatgaat tcagcaagga gtttaaaaag ccgctaattc
    12601 caaaaaaagc aatattaaaa aactttaagc caacaaaagg cgtacgagct aacggtatgc
    12661 ctggtttata atcaaaaaag gcatataccc atccaaataa aggtatatag gaaaatataa
    12721 aaattaacag aataaagggt atagccatga gtaagagtat gagcctatct ttcattattc
    12781 tgtttttttt gtgcatctct attccatttg gttttatcat aaaatatgct cctaaaatac
    12841 agatttagtt taaaaacatt ttcaaaataa ttacatgcat gataaataca tttccattac
    12901 agtaagtatt tttactcttc caggaacttg cttacacata gtattataaa cactatattt
    12961 agctaataaa atgaacaaat caacaataaa tgttaaaaaa cacttcagtt ataagaactc
    13021 atattttaat tataaaaatt atggaactat tctacgattt ttggaatata tcttgcactt
    13081 catcttcacc aattttgggt agtcttaaaa tagcacttgt cccagaacct atcttacttt
    13141 gaattttcaa gccgtatttt actccaaaat acaattttac tctatggtct acatttctta
    13201 tacctattcc ctttaatgat tgactatttt ctaacaagcc agagcacatt tcctctattt
    13261 ctctgagctt ttctaccgtc atccctgcac cattatcaga gattgtcacg attacattct
    13321 cgttttcttc agctatttct atttttattt ctccattata gttcttttct gccaaaccat
    13381 ggtaaagggc attttcaacc aacggctgaa ctataaactt taatatttta catttaatta
    13441 agtcttcctg aatattaaag actattttta ccccttcacc ataccttaga ttcataattt
    13501 ttatatagtt ctttaaaaaa ttgaattctt cctcaataga gtgaattggt gaagggtctt
    13561 taaaaatggg agacaatata ttaccgaata tggttatgcc ctcaacaata tttttggcat
    13621 ttatcataac agccattaac ttgatggtat tcaaagtatt atatatgaaa tgcggattta
    13681 tttgtaattg tagaactgct atatcttgct ttcttttcat ttcttcaatc attttattct
    13741 catgaattaa cttttctata ctaattgaca tttcattaaa ctgcctgata atcataccga
    13801 attcattttt aggagactca gtaatcgtta tgccaatatt ccctttccca acctctttta
    13861 ttgcattaat tattcttttt atgggctttg taaaaaccat tccccaatat gtagataaag
    13921 ctagtgatat tgctatacat gcaaaaaaca taagtattaa tatatttcgc attgtatgaa
    13981 tatcttttat ataattttta taatcaattt catttattag cgtccaatcc aaacttgtta
    14041 atttcagata aaaaatttgt tttgttactg aatcaatttt taaagtgaag ctgccgttat
    14101 caagattttt atctagctta gtaaaaataa aagagttgct tcctattcta gttgcgtcaa
    14161 tatcagaaat aattttacca ttagagtcca caatatatgt actgcttaat ggatcgtaaa
    14221 tattactata tattgatgtt ataacacttt cctgtatgtt tattacaagc gtagcctttt
    14281 gcaagttctg gtaaaaaggt ttaatacccc ttacagcaga aataatatta atattctttc
    14341 tactgttggg tattttatta agattaaaat cctcagattt tccccctcct gcccatgcaa
    14401 gacttgagct gctacttttt gccaatttgt acatatcaga actataaaat ccattctttt
    14461 tattagaatc gttcaggtat tcattggtcg aagctgttac tcccagtatt ctcccatcct
    14521 cagtaaaaat aaaaatagag tctatataac tatagttttg tattatacta aacaattttg
    14581 aatacaaatc attacacact aaaatattgg tatagctgtt atcatactca tactcttgct
    14641 ccagaaaatt ttgtagtttt tcatcaagta aaagcaattt tgatatttta tctacctcat
    14701 tagcaaatac ttgaatatta taatcaaatt ggcggaattg tttctctgta aattgatctg
    14761 aacggttctt taaaatattc agtaccccta tatatgtgaa tattgaactt aataaaagta
    14821 caaagaagct agtaacaata aatgatatgg taagttgtat cttaagcttg gaattggcaa
    14881 tttttgaact aatagcatta ataatattca ttattactcc cccaagactt gcatagactg
    14941 ccttcggaat ttattaggtc ttatacctgt ttgcttcata aatgagcgac taaaataact
    15001 gtcatctgta aacccaacct tttctgcaat ctcatatgat ttcaaatcag tatttgataa
    15061 cagttcttta gctttcgtta ttcgaatata gttcaaatac tcaacaaagc ttaacccaat
    15121 ctcccttttc aataaaccac tcaagtaatt tggactcaaa tttacatgtt cagcgacctg
    15181 ctgtaaagta atatcctctg gataatgcac ttttataaat cttgttgctt ttgccacttc
    15241 ccgacttagt gcttttttct ttcctgcaat attattaact ccgtcgatat actctttaaa
    15301 aacttcaatc atctcttcaa acgtttcaga ataatgtaat tgttcaagac atgatcctag
    15361 tagtacatca atatcttctc cgtttaaatt caatgaaaca tatagccaat atatccattc
    15421 aacaaataga ttttgtactt gctctttaga cgatatatcc tcagattgta taaatgactc
    15481 aattcttgaa ttaattccat ttctaatcag ctcattaaac atatatacac ctccacagat
    15541 ttccttcaat gactctttgt tattctcata agctttccta acaaaggatt tgcttgagtt
    15601 agttatgtat gtatccaaac ccaaataaaa tttttgtttc aatatatcta aacactctgt
    15661 atataaatct tttaatgaag caaatccatt ccttaatccg cttataccaa atgtagccgt
    15721 agaattgaaa taagtcttta atactttttt tatgtgtcct agaatagtat atagctcttc
    15781 ctgtatgctt tgttcactgc taatatcatg aaaactaaaa agaagtatat aatgactatc
    15841 atctacacgt aacgcttcta tcctttcata ttggttagat atttcatcta aaatatttag
    15901 tatggattgg cttaatactt gaccattctc ataactatag ccttttcgta cttgttcgaa
    15961 atgatctatt tcaattaggc acataagtaa attattgggc ttaatccgta gttttaggcc
    16021 aattacactt tgattaaact catttataga gcaattatta taaaatatta attctttaat
    16081 cattttctct tttatcaaat tattatctag tgttaaagta ggctttccct catgaatacc
    16141 tttcaattca tcttgggctt ttgacagaac attttctatt tcctcatttg acattgtcag
    16201 ctttagtata taaccagata tattcaatga gactgcttgg cgcaatagct caaaatcttc
    16261 taaacaagat aatattataa caactgtttc cttatctttt tctctaattt ttttgattag
    16321 ttccataccg tctataatag gcattttaat atctgtaata atcaaatccg gtttttcttt
    16381 tttatatatt tcccatgcag catgtccatt ttggacgtcc gcaatcacgg tcataccata
    16441 cttgttccaa ttaattgaat tcttcagtgc aagcctaacc agtatctcat catctacgat
    16501 taatacctta tacactacac taccccctca caacaagtat tatatcatgt gcttgtagtt
    16561 taagaaagcc attacatatt aatgccatag ctgaagcatc aatatatttt ttaccaggta
    16621 gtagtcggga tgtaagccat attaacctgc tttttagacc aattccacgc tattgtaagc
    16681 aacaccataa tttcaaaaat atgcaaaatt aaagttaaaa ctcctaactc agcatttagg
    16741 cataagtaac ccctctcaag tacttgagca tccaaatcac ttatttccgg acatgctacc
    16801 attctgacaa actcaagctg tgatatcata tcctttactt catcctacca gttgaaaaga
    16861 tcaattagct cataatcttt aacatccatg gggaagtcta aattaatctc aggtttttct
    16921 cttttagcca gcaaatctct gtccagttcc atctgctttt gtggggccag tacaatttgt
    16981 cccggtctgt attcatttaa attcaacagc ctgtgaatgg aatatggcgt gttgaacata
    17041 ttggcattat caataaaatc aaagaccaaa agatattcct taccttcaca tttcctcatt
    17101 ccacgtccca gttgctgcat ttctggtgta ttgggacgcc aattcagggg catcaggaca
    17161 gtaaaccttt gccgggccat tattggcttt agccacagca attacagtat cactgctcat
    17221 aaacaccaat attccctttg tttccctttg tatttccaga agagttattt ttccctacca
    17281 tcggatagac ctccatcatt ccaaaagaaa cccacctatc aaagtcgttt ccgattaatt
    17341 cccaccaaca actgaaatcg tctgcaccgg cggttctttc gaattcaggc agttcgaaga
    17401 atgtgctata acaggcaaat cccttactgg ttatatcaac tgcttcacat tccttcgtca
    17461 ctctggcttt tgcttatctc ttattccatt ttaatattct gcacaatctt ctttagatac
    17521 ttgagactcc tgtgaattta atcctctaca tcataggttg gctcatattc aagcagatat
    17581 actcctttga acgacatttt ctccagcaaa ggcttccaat ccatggtacc gtctcctaca
    17641 cctgctgcaa cccaggaccc gccgccatat tgtatccagt cctttaaatg gcagtagttt
    17701 accctgttgt tcaatatgtc aaggtatgtt ataacttctt cagtctaaac aggctttagg
    17761 tttcctggat cgaagttaat ccctgccgtt gcaggcagtt catccagaag tctttttaat
    17821 gcttccttgt ctgtagatac ggtcggagta tataccattc cttttccctt tgccgttatg
    17881 gcgccatgag tttcaatgga tattaccatt ccgtattttt taatctaatg atcaactgcc
    17941 ttaaaagcca ttatcatatt attccgcttt tcactcgaca ttttctcagc aggtacaaag
    18001 ccggcaaaca atcttagata tgtcactacg aaggctcttg aaagctttgc ttccatgcat
    18061 atccgttcaa ccgttttcca aacttcgttt gcatttcctt tggtaaaatc attttcaagg
    18121 cacataaaag gtgttttaat accgtagtcc tttatacaac tcaaagtgtt gtttaattca
    18181 gcttccgtca tgtctcccct aagtctgcca ataaagtctc caccaattcc aagcttcata
    18241 tatttgaagc cgaatttcgc ggcgatcctg cagtgttctt caaaaggaag ctcacggaac
    18301 ccccactcgc ctactcctac aatcatgtct tcaactcctc cttataacgt atcgatttaa
    18361 gcgaaaaata caactcgtct acacttacta ctcctcctat tgtaaactca tatcattaag
    18421 tctgcaggat tcaggcaact ccactgacca cggcatgagc gaatccaaca tactctgatc
    18481 cttggcatca acattaggaa gtgttttcaa caaataggcc aagtattcaa acggtttaag
    18541 attatttgcc ctggcggttt caatcatgct gtaaatcata gcacttgcag tagccctgtc
    18601 cggagcgttg caaaacagga agtttttgcg tgatatcaca aagcctttta tactccattc
    18661 cgcttattat tgcatcatat acagccgtat tttaagataa aatctaaatt acaaaattac
    18721 tcactttttt attaaaaaca atcacccttg aaactccctc tgcattttca cttttccagt
    18781 actatcatca tataaccttt tacctgtggt agaattatat ctgtataatt accattctgt
    18841 gcgatatcaa tagaggtctt atatggtgct atataagctt ttttatactt gccttttgca
    18901 tatacaatgg cacatgcagg tataacctga tgttcttcaa ttatatcacc tcgttttacc
    18961 ccctcatgtt gatattacat gaaagattat acatgcaagg tgggggattt ttcaaccgaa
    19021 attgtggtgt acttttagat taacacaaac aaccaaccat acttgtatcc ggttccagaa
    19081 accatgggac gtacaaaagt cagcatatca tgggaaaaca gttttgtttc ggtatccgtc
    19141 cagtatagcc gggtataatc atttttcaac gtatcgatat atttcctttt gatgctttcc
    19201 gcgatgcttt ttacgttttc tgccccaaat gaagtgaaat ccgcatacac attgaaattt
    19261 aagtcatata ccataaagat gcattcatgc ctccgagtta gtgtctaaga tgaaaaaagg
    19321 gtcttcgaca ctttatccaa atccttatat tttgtaaaca catctgaact gtaatcatta
    19381 gttaaaagct tgtataggat tgtattaaac accatgttgt tggccacaga gtacactttg
    19441 tccttgacta cgcttatagc gtcggatgca atgcgaaggt ttgtgtggat attattgtaa
    19501 aagtttttat acattatgtc ctgtataacc gcagagctga ctcctgccag cagaagctcg
    19561 ggaataacag taccgcatac cagtatgacg atcagcttga cgatactcct gtgaagccac
    19621 tttaaaatgc gttttctcat ggttaacctc cgatgtatgc tcggtatagt tccctctcca
    19681 gcatttctta tgtgtcttat tcaggcattg taccttttaa attccgaaaa gttcacagat
    19741 attgttttta acatattcca tatccatctg gccgcatacc tttgaaacat ccacttcagg
    19801 tggattacag ccgtctttca ggcaaattgc cgccgcagtg cctgccgctt cgcctatggc
    19861 catcatcgtg cgagaaagtc ggcaggatgc gcctactata tgtgtaaatc ctgaacctct
    19921 gcaggaaact agcaggttat cccgttctac cggcagaagg catgagtatg gtatcccata
    19981 aggatattta agattttggt cgtcgttgct tgaatgcttc ttgccatgta catcaatagc
    20041 gtggtcagca tacacaatta tttcatcgct taatggctgt tcaccaaaag gcatgcgaac
    20101 ttcattttcc gtcaggacat gcctggcctt gagacgatag gtttccctta tgccaatgcg
    20161 aggggcaaag gatatcagcc gataaccagc aaatcctttt tcctcctgca accatttaaa
    20221 atagcgatag acccgtgctt tacacagttc gtatcccttt ccatattcca tatcgaacag
    20281 tactctccct tcaatagttg ggagcatatt aatacagtaa tccccgttgg gatactgggt
    20341 tacaaaggat accgggcgat tatttggcag attctccttt gcccagtctg atacgtcaat
    20401 gctgtcatag gggctgggaa tagggtctac gtccgattca tccttttttt gtattctgaa
    20461 aatcaaagaa catgcattga gataattttc atgttcttcc ggcgcggaag gctcgccgta
    20521 ctctgaggaa gcctcgcgtc caaggttcca gccgcatccc gcgtcgcggg caagatatat
    20581 gtctgccgac gcatctatat atacctgccc tgatacagta aattccctgc tgtcctgctt
    20641 gagcacaagc tcttttattt ttctcttctc ggtcctggat ctcacaaatt ctgtattaag
    20701 aaggagtgtc acattcttcc tatccgatat catatccttc atgacccgat ccatggccag
    20761 cggttcaaaa tggaaacgtc gttgatccca tgaatccact ccggctcggc gccgggtcct
    20821 ttcatagctg tcactgcatg gctcagatat gcccatgggc ttttccggcg tcatatgcca
    20881 aacatccttt gcaacacagc cttttccgct ttccagaagc ctttttgcta tttcaacgtg
    20941 gtaccggcct gtggatacgc ccggctccca gttgtgaacg ccccccactg tagaagtacc
    21001 tccaaggatg gtttcacgct caacaagaag tatcgacgca tccggatttg tatccgacgc
    21061 tgcaaggcag gcccctactc cggaagaacc gcctccgatg attattatgt cataatacat
    21121 ttaaaacacc tcattaatta tatcgtcggt gttcagtaaa tccgaacacc ttattttaaa
    21181 cagtttataa taactggaaa taaataactt cacctcacag catctaaacc ttctataata
    21241 gtagtttaga aaggggatga ggtcatttgt tacccaaaaa tcaaactcat ttagcttata
    21301 cactaactcc ttttcagcac gtaaaatgtg ctgatagtat ccgcaaagat tatttcaaaa
    21361 gttgatcttt catgtgtata tgacctttgt aaacattttt attccactaa tcaacatagt
    21421 cgtattgcag attatcgacc acaggatgcc ttaaggtcat tacttgtgat gtatcttgct
    21481 ggttttacat ccattaataa atgggtaaaa gaacttaagg atacaccacg ttatgcttat
    21541 atgtccggtt ttagcccaga taaaactcca tctaaagcct atttttcata gtttacttca
    21601 aacctgttta gcaaagatta cgatattgat gatattatga ctcagggaac tctgcttaaa
    21661 aaattttttg aaactgcatt tgtcgataaa tctactgagt taggaattaa agatcctcat
    21721 gacaaagcta tctttgacgg ctgtaaagca aggtcttttt gtaaacagag atataaaccg
    21781 gataaatcat gcaggtgctc cagtaatagc attaagcctt ttaagtgctc tatgtgtaca
    21841 gatccagagg ctaccagagg ctacgatcat aaccttgatg agttcttaat gggttatggt
    21901 ctgcattttg cagtaactcc actggttaat agtccatcca aatgtctgct acttactttc
    21961 tttgttacag gtgccgacgt ccatgactcc aaagtatttc cacatctttt aatatatctg
    22021 tatcatgata tgaaagtgga ttttaactac gttatcctgg attcaggcta tgatgcatgg
    22081 tacatttatt ggtggattag tcttctagga ggtaagccta taattgctat aaatcctaga
    22141 aactcacaaa ctcctacacc agagaatgaa tatggattac ctgtctgcca tgcgggccac
    22201 gcttactatt actggggaat tgataggaaa aaacgtagat ataaatggcg atgtccatta
    22261 aaagcatcta aaaagttgtc taaaaaactt aagtgtgata aaccgtgttg taattccagc
    22321 tatggtaaga cagtctatac ccacattgaa gatgacatcc gctggtttac acagttccca
    22381 agaggatccc aactctggaa agacctatac tcaagaagag cacgtatcga accagttatt
    22441 ggctattcag tactaaatta cggattaaaa acccctttat ttcagggtac taaaaacttc
    22501 ttcgtgagga ctgccttagt ttgcatgatc caacatgcaa aagccatagc tagtaaactc
    22561 ctgagtatag aataccaaaa aactgctgct taatccatag gaaatttaaa ccttaaacct
    22621 atgcttaggc aagctttgtc tttatctctt ggaaaacact tcctatcaat tgcatctttt
    22681 taattttcaa agatcagtta ttatcttgca gctcacttct tattcatcac aacttcattg
    22741 gtatttattc ccaatttgtt ttaatttact gaacacctac tattatatta caactagttt
    22801 gtactatttt gtagaaacat gtcgaaatat ttatatggcg caatgtgctt taagaaatgt
    22861 tattaaaaga aattgctatg ctttaaggct ataggcttgg ttgctaatat tatcctatag
    22921 actttcgcta tcaaggattg ctatcaatgg atatgctatg tataaagttt agaattctaa
    22981 gtgttgctgc agcgaaaggt ctttacggca atcttagcgg tataggagaa tcatagtatg
    23041 ctaaaaaagt ataccacaaa tttgttatgc tttcctcata gcgattgtat tatttggggt
    23101 aaggttaatt cttaacgttt ctaaaacacc cacaaaaagc aaaagaataa acaaaaaact
    23161 tgggattcaa tattagaaag aaaatcaaaa ataatattgg cattattgtt aattcccata
    23221 tcgtgaataa tagtattact actgctatca ctgttagaaa acgaattatt ttttatcatt
    23281 agcatcatta tgcatttata tcacaaatcc tcttttaagt ttattaatat cttctgcatt
    23341 gttttgccct ttctggctta ttttgtattc tctatcagtg tttcatcaaa cattttctct
    23401 atatctctta tagttagttt taatgctgtt ttacctgata tagaaccagt aagcctatta
    23461 atgaattgct gtcttatcta ttgcataaac tgctaaatat tattgctatt gataacatta
    23521 aagctgacat tagtattcag actgccatga ggctcttttt taataaaatc aatactcatc
    23581 ctatatgccc cttttcaagt ttcttaattc ctttgcaata atataatacc catttatatt
    23641 tatactctta gattatttct caaccttttg ccttcctaac ttctcttcaa ggtctgcaat
    23701 tattctctca aattccttaa tcttgccctg gagctcattg ttttgcaatt ttaactccgt
    23761 tacttcgttt attgtattat ttctctgttc ctgcagcttg tctatttgtt ccataagctc
    23821 tttataatcc ttttgatgtt gcaactgtgt ttctttaagt tctttttgat gtgttttttc
    23881 ggctgcaagg atagcttttt ctttttccag tatagaatgc tctttttgtt tttcgatttc
    23941 agccattagc tgtttattct cagtcattaa ttgattatta tgagttttaa tttcatttaa
    24001 cttagatatt tcagacaatg tgttatccaa tttttctttt aactctttga ttttaccttc
    24061 tttttcattg cagctgtctg tcaatgcttt gtttaggtta tccttatcgg ctagtgtagc
    24121 ttcatattct ctattttgtt ttgaaatttg cttttccaac tctgaaattt ggttcaataa
    24181 acggtagttt tcttgttctg cttcttttgc agtttcttta tataattcat ttgctgcagt
    24241 aagttctgtt tctttattct gtaaatctgt aataatacgg tctttgctgc tcagcaagtg
    24301 ttcaaattct gaccgtatcc tttcttcggc atcttcgtta agttgtagtg acattaaaaa
    24361 catttcagtg attttattaa ggtaggtttg aaagctttct atctctattt tcctttcaac
    24421 cagcacggcc ttactttttt cggtttcata taaggagatt agtgatgcta gacattgtcc
    24481 ctggttacca aattcctcat ttgctaaagt cttgaacttt tcaaaggttt catcatcagc
    24541 cctgaagctt cttaccttgt attctttcac aggttccaca tttttctcct ccatttgata
    24601 ttttataatg taatacgtat acaatacatt atgttacata gtgtattact cccattcgga
    24661 aatgtcaaca gttattatag tatgtaatag aaaaaagatt agcagctgga tttatatttt
    24721 tcgcaccatg caataagcat ctctatacac ttcataatta ttcctttttg ccgaactccc
    24781 ttaacagttg tccttctgtt ttttgatctg tcaataactt tgttaaatcg tcatgtgctt
    24841 catcaacact actatactca ttcaatactt tgcataactt ataattttct tcacccattt
    24901 tgtgtactag gtatactttc ctgctattac catataattt catttaatat ctctcctctt
    24961 ttctttgtat tccattaaat actaacaaaa catacttatt tatacaagca aaatgaagtt
    25021 agttttagtt tgtcatacga tatattctgt gctataatgt ctttggagat tttgtaatcg
    25081 cctatttttt ttggcaattc ttttttttgc ctattttcag atttttcaat tatatcaatc
    25141 ttatcaatct tttttttacc atcataaata tcagtatcac taggtaaaag atttttacac
    25201 tcttgactga aagttttttt cactctggag cgaaagattt ttacaccccg gtcatatcaa
    25261 ggcttacaga gcctatagag gttaaatata tgcgattcgg tctatttaat agaaatttat
    25321 gcatttgata aaaacgatta taaacaacat aattttttgt ccaattcgag tttggcattg
    25381 aatatcgaaa aggcacaaaa ggttttgaag aaatgagata tattagtttt agaaattctg
    25441 actcttcatt tgggatagca caagacgaga gcgagcgttt atgaaaaagg tataagatgt
    25501 ttactttttt aaataatgtg ttataatata gatgaaggcg atcggtaccg caaagtacgg
    25561 ttgccacaag ggattataat aaattaataa tcagcctgtt gccgcagggt gattattttt
    25621 tttgtcctaa tattgcctta actatagcaa gtattaagga gacaaacgat attactaaca
    25681 tagaacttgc tatcattaga gagataacct catatgtatt catatcgcgc cacctccttc
    25741 ctctaaagga ataaaggtgg caaccacacc ctgcttaatt ccgatcacct tgtccatatt
    25801 ataccataga gatatgcgtt tttcaataaa ttacggtgct ggctattttt gttttagcac
    25861 aaatatttat aaaaaaaaca ggctatttta gcctgttttc tatatattac atatcccttt
    25921 tgctttaaaa cttatatact tattgtcttc gccgactata atgtggtcaa ttacctttat
    25981 ccctaatata tctccggctt caataagccg tttcgttatt tctatgtctt ctgtgctttc
    26041 cgttggatcg ccgctagggt ggttatgaaa taaaataata gcactcgcat tgtttaatat
    26101 tgcctgctga aaaatctcac gaggatgtac aatgctactg tttaaatttc ctacactaat
    26161 tatatgcaca cctgctattt tatttttaac tgttaacgtt atgataccaa acttttctac
    26221 tgttgagtta ttgatatcca atactttttt tattacatca tttgctattt gtgaatttcg
    26281 tataacaaca tactccaatt catacaaccc cgttttctcc tttattagcc ttacactgta
    26341 tatacttatt cttttggcca tctaatatat cccccttatt atattgtatt ttttcttttt
    26401 tgtcttgcta ttaggcaact ttatttaaaa aataactaaa taaatctagt tgaccttcca
    26461 tgatcttttt tcttgatttc ccgtttttgg gttttgatgt cttaaataat aatttgagtt
    26521 ttgcattttc aatttccaaa tcatctatta tatttgtagt tatatcgttt tgttcttttt
    26581 caattattcc taattgcata tcttttactg atatgttttt atcttttgtc tgtacctgct
    26641 ttttggtatt cagtattgcc atctttttgt atatagcaga tacatcctct acgtttgcac
    26701 ttattcctga tgcaagttct cttgccagtg cagttgttaa atcctcgcaa tcatttagtg
    26761 cagccaaccc ttctgaagtc acgttgcctt ctattgttgt tgcaacagca agttttgttc
    26821 ccattaatct tattgccctg tgctgcattg ttccctcata tgcaaagata tagacttcta
    26881 ctcttggtga tgtttgattt atccgccagg atcgtcggct actttgccta aaggtgttta
    26941 aattataagc tacattgtaa aagattatgt tggtaaactc attcaagtct agtcgggtag
    27001 gaacccgtcg ccttgccata tttctatggt aggtttacga gcgcccccca cagaaccgga
    27061 cttacacttc tcagcgtatc cggctcccca tctacctatc agtctaattt tcccgcgtgt
    27121 aatttatcat gacacgtcgg gcataaagcc attgttttac gtttcctgcc tatcataacg
    27181 atttcccaac ctgttttgtc tttcaagtcc ttaatcttcc tgacatggtg catttcgata
    27241 ggaacatctt ccgcaccaca ccattcgcat ttctgggctt ttagtcgact gattaggctg
    27301 gttctattga gatttctgtg taccaaaggg ataacgtcaa cgtccggcat tttgattata
    27361 ctgccgtcat gtctaaaacc gccgtcatac aaatacgcaa ccgcttttcc gcttttgctt
    27421 ggatatttca cgccgaaacg cttatatcca tactttttac ggattttgct tatcctcgtt
    27481 ttgtatttgg cggcaaaggt cttaaacata ctgtatttca taacatagta gaagtcgccc
    27541 aaaacgcttg cattatccgc aatgcggtaa tagttgtaaa ttccgcgaat ttccgcattg
    27601 tattggttta aaatctcaag gtcgtcactg ttgattaaat aagttctttc gcatggcaca
    27661 aatatttcct tgccattttg atacttgatt ttaagagcag agtaatctgt aagttttttc
    27721 tgccactttt ccctcgggac gtatagcttt attctgccgg tttggtatct ggcgcgattg
    27781 ccatttttgt cttcaatcgt ttgttgactt tgtgacgtgc atatatcaaa gcctaaaaaa
    27841 cgggctttgt catgcgcgtt ggtaataagc gtcttttctt cggacagttc caatttgagt
    27901 tcttcggaca aatatttctt aacatccgct tttatcattt cagcgtcctc tttactgcca
    27961 atcaccccac aaatccaatc gtcagcgtac cgtaaatact gtaatctcct gtaattgctg
    28021 tccattgggt ctttcgtagg cattttaatc ttttcattcg tcatataacg aatgtttttc
    28081 aaatactcct ttttgttatc attgcccata ttttcccaca tttctggcag tattttacca
    28141 tctttcagcc aacggatttt ttcgtgcaac tgccaatacg gtttatattt ggcacgaatt
    28201 ttctccgctt tgaaactttc catataccgt tccataaatc tgtcaaactc atttaggtag
    28261 atattggaaa gtatcggact gataattgag ccttgcggtg taccggaata ggttctgtaa
    28321 aacgtccaat cttcaatata gcccgctttt agaaatttcc atatcaggct gatgaaatat
    28381 tcgtcatgta tgcgcctgcg tagtattcca acaagtacag cgtgattgat attatcaaag
    28441 aaagaggtta tatctccctc aatgaaccat tttaccgctg tgaaatttgc ttttagttgt
    28501 tggagcgccg tatgacaact tctgtcagga cggaacccgt gggaacgatt ggagaatgtc
    28561 ggttcgtaaa tgctttcaag tatcatacga ataatttcct gcaccagctt gtcattgaaa
    28621 gatggaatac ccaaagggcg caactttccg ttctttttct tgatataggt tctgcgtgca
    28681 gggtacggct gatagctgtg gtctttcata gaagcgatta acgcctcaat tctttccata
    28741 ctcatgccgt caacagtttt accgtctgta ccagcggtca tgttgcctgg tttagcgtaa
    28801 atattctgat acgcttccgt ataaaattcc gcattataca gatttcgata taacctttcg
    28861 taatgataat cagggttttt cgattttgac cttagactgt ttaatacatc aattggattt
    28921 ctcatggtgt ctcacacacc cttctaatat ttttgtatta agaggcaaac tacctccctt
    28981 cgccatgtgg acgggtttcc cgccctcgaa ctactatgga ggctccgtta ccatgccgga
    29041 ttttcagcgt catctttctt gggcttctaa cccttgaaat ttatcacctt tcggcattac
    29101 gcatagccaa aattatattt ggcgttccag tttaggtaat ctccgtttag actgatagaa
    29161 ataacttgtc aagttgtcgg atatggcgtt tgtcctttac tgtttatcac agttgaggcg
    29221 gtaagaatat cacggacaat tccaccagga ctctatggaa ctcccatacc gcttgacgct
    29281 taaactatcg tgcgccaatg attcgagtag aacctctcgg actaccattt aggcaatcaa
    29341 gcttttatcc tcataaatga catttcacct tgcaattcag tctcggcgtg gatagttctg
    29401 ccgacttatc gctttaccga tatgctacac tccccgcgca gtttcccttg cggataaatc
    29461 ggttggtgat aacgtggtta tttcaacttt gatacttttc gttaccgctt tgttaaagca
    29521 gaatttctat cagcccgcgg acacttgccg ctttaagcag caatctgtgt ccttacggac
    29581 gcacatcctg tttctaccag gcttggatta gttactaaaa catcaatctc atttgcattt
    29641 ttcatcagcc attcttctct tttgcttgcc tgtatagacg aggatagtat ttttgttcga
    29701 taatcttttt cggtcaatat tttttgcaat tttttttggg tatcggtatt gacccaattt
    29761 gtataaacta gtaatttttc acctttagat attttttcct ctgcaatctc taataccttt
    29821 aattcctttg cataaagatt atcaatgctg cctatgtttt taggtacaac taattcactt
    29881 ccgtcttcag gactatatat aggcttcata ccgtaaggca tgtccggata taaggttaac
    29941 attattaaat agtttcctaa aattttatgc gatatatttc tttcgctttt aagaatgctt
    30001 tttagctcgc cttctattct ttcgtattca ttagctactt cctcatccat tttcataatt
    30061 ataggaattt cctcatattc cggcattgaa tccgtcatat catctaaaga taggaatact
    30121 gcattatcaa gtaagtatct cgtaaagact agtggagata caccaggtag tagtttttct
    30181 cttagttttc ttttaactgc tcggctgcct gcattgtacc ttgcttcttt taacttatat
    30241 tcttcttgga atacaccgta ttctttgtta aaggctcttt gatcgtagta ttcatagtcc
    30301 tcttttacca tcaatgaagg aaagcatcga aatagtatat aaaatatact actactgtat
    30361 ccatttatta aagtagcagt catggctatt acttttttag acattttggc taattcccct
    30421 gctgcaatac cttggcctgt atcaccttta tataagtgca actcatccaa tatacagcta
    30481 tctactacat gctccatctt ctttttcatg taagtcgata atccatattt tctgtatgca
    30541 ctttttgttg gtatgtaccc tgtctttgca ataatatcgg ataattgttc taatgttttt
    30601 tcgtcctcaa cttctttaaa gtaatgtttt gccataggtc tgtataccca gccatatggt
    30661 gatacttttg cccatttatt atatctcctg tcagatggat ttacagcagt ccataaaagg
    30721 ctgttacaat ttttacactt acggtttgca gagttctcat ttctgtaata aaaacaatcc
    30781 gcatctacca tatattttac gtcatcctca gttaattcca tctcttgttt tgagaaacaa
    30841 tcagggcata taaagcattt ttttgcattg ctccatatta ctgctggttt tttcatgtat
    30901 ccgtctctag cacgttcctt tgagataaat acatatactg tttttctatt ttctgtataa
    30961 tattcgtatg ctttgtttaa ttccgttata gtctgagcaa taaaagcttt tgaattggga
    31021 atagtccatt caatttccct tacccatttt tctcctagat ggccaggtcc taatattata
    31081 ccgaaatgtt tttctttttt atttttataa agcgaataca gtgcagtgct tgctattact
    31141 gtttttccgc tgccacactc agcacatagt acaacacatt tatccttttc gagctttttc
    31201 ttcatagatt ctgcaattat tttttgtgca gaatataaac ggatacctct tgtttgatac
    31261 aaataattgt ctatttcttc tacttctttt gagatttttt cttttgtagg atcaaacaat
    31321 ggtacaaaac tttttttgat tttttcggca atttcctgcc cgaatttact taaataagtg
    31381 gttatatctg atatgcctaa aaatcctttt tctttaggtt ttatgtctga aatttttaaa
    31441 tgtccttcct ttaaacctgt ttctactatt ctttgtattg tgtcatcatc tcttatcatg
    31501 caatatgcat ctaaaaggtt tgtgttcgat aaacactcta atttatataa tatttcttct
    31561 tcttgcattt tttctattac ataatcaacg tattcatcta acattggtat tgacgttctc
    31621 ttgtttatct ccttaaataa ctgctttttg tttttatcct tgataaacaa gtatgattta
    31681 cgcaggatgt tttcttcttc attatcattt cttacataat tttcatcatc cttgcgtagt
    31741 aatagacact ttgtataagg atcatctttt tttatatttt ttttataggc tttagctaca
    31801 gaattaaagt tcattatatg agtttctgta tgaactttaa ttccgccacc agaacatatt
    31861 gcatcagcca tcgctcttac ggtttccgta tatcccccgt agtacataag tacaagctga
    31921 ttagttctac tatcatagac tatgcattct gcatagtttg taacataaat atccttatca
    31981 tagtttgtat actcaacctt aaacaagcca tttatcatgc gatcattcct ttcttttttt
    32041 tgtcacaaac aaaaaagcca acagtaaaga atgtttttct ttattgttgg ctttttacct
    32101 atttttttag atatttaaat gttattctgt agcaggaaat accgtactta aatctatttc
    32161 tagatccggg aatacagaaa catttacttt atctgcttcc gtgtaatgtt ttaatcttcc
    32221 ataccttttg ttttcttgta gagtaaatac cgtaacaatt ctatattccg gttctataat
    32281 ccaatattcc ctaactccta cttcctcata tttattaaat ttttttattt tatcgttccg
    32341 gaccgttgat ggtgatgtta tttctattat taatgttgga ataccatgat atcctgtccc
    32401 ttttaattta gtttcatcac aaactactac taaatccggt tgataaacgt tagtagttcc
    32461 ttcatctgtt tcatctacat cagataatcg caagtcaaaa ggtgatgcaa atatttggca
    32521 aggcttatct ttcaaataat tattaaactg agttaataat tctcttgata tagcttgatg
    32581 ttgccaagtt ggtgctgctt gcatataggg tactccatca ataatctccc atcttttatc
    32641 ttctggccaa gtcaagtagt ccgcataagt atattgctta tcttcttttt taggtaacga
    32701 catctaaaac actccctatt agctttttaa atagtgaatg aaacattata gctttcttgt
    32761 attaaacatc tcattcaccg tttgcattgc aaattccgca agttctattc ggctttcgag
    32821 atctccggta taaattgatt caatttcggc cattttacac aaacggttta atagcttgat
    32881 attaactatt accatatcat gtaatctccg tctatttacg tctagtctta cgatgtattc
    32941 acggtaggct tccggttcta gcctgaatct cgcacatgtt atagctgctt ccatactgtt
    33001 taccgcatca atgtatttcc cacaagaatc gataatgtcc tctatatctg atgctaattc
    33061 gtcttcgttc atggatatta gtttgtttac tagagcattg aatttttctc tcaacattat
    33121 tattttctcc ttttctattc tattatatta tatagtattt ttttagaaaa tcgagtaggg
    33181 ggaaaataat taatttattt tccgacctct cacaccacct gt
//
LOCUS       DAOSDD010000018        33052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_665_length_33052_cov_5.491620, whole genome shotgun sequence.
ACCESSION   DAOSDD010000018 DAOSDD010000000
VERSION     DAOSDD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 33052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33052
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_665_length_33052_cov_5.491620"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1280)
                     /locus_tag="PK733_03010"
     CDS             complement(276..1280)
                     /locus_tag="PK733_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ09543.1"
                     /translation="MPTTIQDVAQKAGVSVATVSRVINNSPAVSEKARNNVMQAIEEL
                     DYRPNLLGRNLRRTETRLILVLLPSISNPFYSKVVKGIESVARENGYNILLCNTDSQL
                     ETEKAYLKLLKSRLSDGVIFMAPELDREELSAIGRSFSVVQCCEYKEDAQVSHVSIDN
                     FSAAYRAVQYLIGLGHKKIGMITCNNNFISTIQRNAGYKKAIEDVGLVYDTSLIKQGN
                     YSFKSGIRAGRQFLLKKDNIPTAVFAISDSMAIGAIRCFREKGLKVPEDISIIGFDDI
                     TYASMCEPMLTTISQPQFELGCEAMELLLMQMRGEVIEPRSIFLDYELIIRESTGKPS
                     "
     gene            complement(1353..2690)
                     /locus_tag="PK733_03015"
     CDS             complement(1353..2690)
                     /locus_tag="PK733_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ09544.1"
                     /translation="MLLILERAITIKNLGKQLFGHKRICGGKYKNSQILFLIDAALIN
                     AAYVLTSGVFLSGFIINLKGSDFIVAILNNAGTWASILTVFSFLIFERIKRRKKLLIT
                     VGALSRFLACSIVFLPLISKDEKLIITLVAVMVITADLLWGFYQLGWMIWYMEIAPEG
                     KKNDYVYFRMFLLRIAFTITTLVMGYVLDFYKKSYTGFLIIFVSSLVLSVIDTVILLF
                     IEEPEYKIPEENKSKKTIFLEPIKSNEFRDFLIFIFAYYLCLTISTSFTSIYLIRYLN
                     FDYGFISTINVITYIVMIISTKFWGRIQSRKGTISVLKYSAFFTIIDFLMYVFLTRKT
                     YFMLLPSALIGGFGNGGFNIAIMAYRFEIMPESAKSIYEGWFKAIYGASVLLAPIIGK
                     LLINVMPDYSGLAFPLSNFQLLYLVSFITGALVVMVTFYKTGFSKNKGTDISI"
     gene            2936..4600
                     /locus_tag="PK733_03020"
     CDS             2936..4600
                     /locus_tag="PK733_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ09545.1"
                     /translation="MEQLMNITLGNLLDEIAEKYPDRDAVIYTDRPFRKTYSQFRELC
                     NQVAKGFMALGVKKGDHVAVWATNYPEWMLAQFATAKMGAVLVTVNTNYKIFELEYLL
                     RQSDSTTLILIDGFRDAKYIDIINELCPQLKNSEPGKLDCPSLPFLKNVIYIGDKKYS
                     GMLNWNDLYKMGRDISDAELAAIQESLDIHDVINMQYTSGTTGFPKGVMLTHYNIINN
                     GKSIGDCMHLTHEDRLCIPVPFFHCFGCVLGIMACVTHGTAMVPIEYFEPLKVMQAVQ
                     DEECTALHGVPTMFIAILEHPDFGKFKFPKLRTGIMAGSPCPIKVMQKVVDLMGASEI
                     TIAYGQTEASPVCTQTRVNDSIELRVSTVGKALPFIETKVVDPATNEDVPPGIAGELV
                     ARGYNVMKGYYKMPEATAQAIDSDGWLHTGDLAVMDEKGYFKITGRIKDMIIRGGENI
                     YPKEIEEFLYTHPSVKDVQVIGVPDKKYGEVILACIILKEGLTATEEEIKQYVRDHMA
                     RHKTPKYVKFVDSFPLTASGKVQKYKMREWAIDELGLRDAASIETA"
     gene            4711..5517
                     /locus_tag="PK733_03025"
     CDS             4711..5517
                     /locus_tag="PK733_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ09546.1"
                     /translation="MKISFSTLGCPGWTWNEIVSMAKDFGYDGIEVRGIKNELYVPKV
                     KAFLPESIEATKTRLKTLGLGISCLTSACNLSDKTNIDTNIKEGKEYIDLARKLEVPF
                     VRILGDPNPHPGENIDFNFTVESLTILAEYAGNNEVKVLVETNGTYANSETILKLVTT
                     VNNSHVGVLWDIHHPYRFFNEPITKTYETLKDYIKFIHVKDSVLVNNSVKYRMMGYGD
                     VPVKEALLILKDEGYKGYVSLEWVKRWNKDLEEPGVVFSHYINYVKNIIK"
     gene            complement(5719..6699)
                     /locus_tag="PK733_03030"
     CDS             complement(5719..6699)
                     /locus_tag="PK733_03030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOJ09547.1"
                     /translation="MSDKKSSEKKYTIAGVGEILWDMLPSGKQLGGAPANFVYHASAL
                     GADAYIVSTVGNDNLGREIINKINIMGLSSRYIQINNNHPTGTVEVKLDNRGIPSYFI
                     HENVAWDYIYSEKEESELLGKADAICFGTLGQRSETSRNTILKMLENAREASKAEDPE
                     NVENAESQCIRVFDINLRQHFYNKEIIHNSLKHADIFKLNDEELPVVSKLLGLGHEIC
                     EEDKNSTLNILWKLAEKYSLKLIALTRGSKGSLLLSEGRVSDHPGFPADIVDTVGAGD
                     AFTAAVIMGFLKKLDLDEINCLANRIGSYVCTREGGTPKLPEELVKRIKQ"
     gene            complement(6809..7108)
                     /locus_tag="PK733_03035"
     CDS             complement(6809..7108)
                     /locus_tag="PK733_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HOJ09548.1"
                     /translation="MQQITYITKGTCSRQIDICYEKNTLVSVSFKSGCSGNLQGISKL
                     VEGMPIEEVIRKLKGINCGGKGTSCPDQLARALESILENTDKSNIDEYELCHVNS"
     gene            complement(7274..8014)
                     /locus_tag="PK733_03040"
     CDS             complement(7274..8014)
                     /locus_tag="PK733_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOJ09549.1"
                     /translation="MLCYTYDGSFEGLLSAVYEAYYRKEYPEKIESEDALQTNFMFKY
                     VHISTDFDKSQRVYNSIIHKISQDALDNIYHVFLADKDKENSTIIYEYLKLGWKVGKN
                     VDLYLSDDRVLRVHQIRQRVGLEVHRMMGFLRFSLLEGNIYYAPIEPDNNILPLLAPH
                     FAERMADQNWLIHDKSRELAALYNCREWMLVDANPGRIPGIDTNEKFYRDLWKEFYNA
                     VTIGERYNLDLHKRLLPVRYWRHLTEKW"
     gene            complement(8035..9402)
                     /locus_tag="PK733_03045"
     CDS             complement(8035..9402)
                     /locus_tag="PK733_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOJ09550.1"
                     /translation="MELIQKLNILADSAKYDVSCVSSGSTRNNTPGGLGTGFSGGICH
                     SFTADGRCISLLKILLTNACVYNCAYCVNRATNDVPRATFTPEEVVDLTINFYRRNYI
                     EGLFLSSAILKNPDYTMELLLKTVRLLRCENKFNGYIHIKAIPGADYKLIDEAGKLVD
                     RMSVNIELPTSAGLQLLAPQKNKESILGPMGYIKSRIEENKEDIQKCRKAPYFVPAGQ
                     STQLIVGATPDNDINILKLSEGLYRRYGLKRVFYSAYVPVSNHPALPSIKKPPLLREH
                     RLYQADWLLRFYGFTADELLSQEHPNFNAVLDPKCDWALRNLQLFPVEANRDRYEMLL
                     RVPGIGVKSAKRIITARRVGSLDFDDLKKLGVVLKRAQYFITCKGKYYQRAEMKDTFI
                     RANLIADGLKLPGAFDEGYRQLSLFSSANENWVGVAGSTELIGANEAVQVKGLIGIDP
                     VKEII"
     gene            complement(9792..10034)
                     /locus_tag="PK733_03050"
     CDS             complement(9792..10034)
                     /locus_tag="PK733_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09551.1"
                     /translation="MPTISFREFDEVCLSLGLEKKMSKKGHIWKGISPINGEYVRIPV
                     HLHSGGRNIATGTLMFNIKELGFKDFYDFQEYLNKL"
     gene            complement(10055..10579)
                     /locus_tag="PK733_03055"
     CDS             complement(10055..10579)
                     /locus_tag="PK733_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09552.1"
                     /translation="MKKVFNKDSEPNPGKEVDKMIRVSNELFSRDIMGVADLRKDLSS
                     VMDNAIDRFQEVVTGNVKKGGKTATIISTELLDLLLGCYQFNPLISYDNPTQQYEVLV
                     PEIGASGFGETKQDAIDVLLDNIVNLTEDYFDDIELYIRIEKYKKQYPYYLKLKGCVD
                     KNQLLKVLGLDRKN"
     gene            10888..12003
                     /locus_tag="PK733_03060"
     CDS             10888..12003
                     /locus_tag="PK733_03060"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOJ09553.1"
                     /translation="MICENTKCIAYIGTYTSGKSEGIYIFEMDTQTGELKPAGVCKGL
                     ENPSYQVIDNKTEYLYTVMETEKFNGEKGGAVAAFAIDKKTGLLEFVNCQPTKGRVPC
                     HISIDSSREYIFAANYTEGTISVFPISQDGSISPVSCIVKHEGSGPNKERQEKAHAHY
                     VSLTPDEKYLCAVDLGIDRIMVYDFDREKGRLYPSKHPEVIIKPASGPRHMAFHPSGK
                     FAYLINELSSDIAAFEYSSSDGTFSEIQYISTLPGESNLPQELSKPLVNYCSAIHISQ
                     DGQFLYGSNRGHDSIAIFKIDQLSGKLELTSITSTGGNFPRDFAIDPSGKFLYAANQN
                     SDTIVVFRITQESGIIEPTGSIINVPDPVCIKFVILK"
     gene            complement(12072..13409)
                     /gene="mgtE"
                     /locus_tag="PK733_03065"
     CDS             complement(12072..13409)
                     /gene="mgtE"
                     /locus_tag="PK733_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOJ09554.1"
                     /translation="MNELINDLITRGKFFDARNEIIKMNVVDIAQLLEELEEDNSLMV
                     FRLLPKDIAAEVFSYMSYEQQQFIIESIADKEIKNIIDELFLDDTIDLIEEMPANVVK
                     KVLKNTSETRRKLINQFLKYPDNSAGSVMTIEFIDLKKEMTVKMALEHVRETGLDKET
                     IDVCYVIDSNRKLQGSLPIRKLILSDGNVIIADIMDTNVISVYTHDNQEEVANLFKKY
                     DLVAMPVVDNENRLVGIITIDDVLDIIERENTEDFHKMAAMAPSEEEYLKTSVFDHAK
                     HRIIWLLVLMISATFTGNIIKKFQGLLQSMVSLAAFIPMLMDTGGNSGSQSSTLVIRG
                     LALGEIEFGDIMKIIWKELRVAIVVGLTLAGVNFLRLYFLEKTDFYVALTVCISLLVT
                     VIAAKVIGGALPVIAKKLNLDPAIMASPLITTIVDALALMSYFSIARLLLGIS"
     gene            complement(13646..15154)
                     /locus_tag="PK733_03070"
     CDS             complement(13646..15154)
                     /locus_tag="PK733_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOJ09555.1"
                     /translation="MRKKRIKVFSDFKILIVDDDEGIIDSLSMLMKRSGYNITGLTDP
                     VKAIEKVRNEHFDLLILDYLMYPIHGDEVVEKIREFNNELYILLLTGHKDLAPPIETI
                     KELEIQGYCEKSDKFDQLLLLIESGIKSISQMRTIKKFRDGLNKILQAVPKIYQLQSI
                     GNILEDILKEILPLVNSENAFILVDDTTNIDSGCSNKSIFKGIGKYKAEMKDFMKMLA
                     PDLIEHIGNARMNQKVVKLEHGAILPLINEFSEAIGVIYADSDDIEEGIKLLEIYASQ
                     AASSINNAFLHSLVNMKNEELNMTYEQLRIRYMDTIEALRQAVDAKDIYTRGHSDRVS
                     YYAVRIGKALKLAEEELELLRISGIFHDIGKISTADDILLKNDKLNEREYKEIKKHPL
                     KGARILAAVSMFKEIVPIVKYHHERIDGYGYPEGIKGNEIPFLSRIISIADAFDAMMS
                     DRLYRSRLELDEAVKQLILGAGTQFDKKVVDVFTRELKNFEDMEREIASLYE"
     gene            complement(15166..17001)
                     /locus_tag="PK733_03075"
     CDS             complement(15166..17001)
                     /locus_tag="PK733_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOJ09556.1"
                     /translation="MLADLGFVLIHVSLILMLAIFYIVMRMKGKKQIHYAFLALLGTL
                     LIWALGAIILEYDHRFNKPTNVLIVNISYVGLILTPVAALFFGLVFANTKIKLSWKYG
                     VFLIIPLISIILLFTNDYHHLFYNYVKYKELTHANALGKYFIIHTIYSYLCITACMAY
                     LIYFSVKNAGFFSKQSIFIAIGIIISFGYNFLLTFQIVRGYFYTNVIAFFFSFLFFFL
                     AIFKFDFLNVVPIALQNVVDHISDSFIVIDKDYYIIDYNKTFTDNFGNILKIKRKDNL
                     TNLINIYKSTDKNISSLFNLLKKAVAERTIVSFEKNIKIGSFQKYFLIEITPIVTQGI
                     YLGTIILLKDISQAKKDLETIQRNYEILMEQERLASLGQLIGGIAHNLNTPIMSLAGG
                     IEALKDLAEEYDESIDDKSVTFEDHHEIAKEMLDWLLKMKPYCSYMSDVISTVKGQAV
                     KLIDSTTIKFTLEELVKRIDILMKHELKKSHCVLNIDIKVDPYTEIKGEVNNLVQVFD
                     NIIINAIHAYEGNGGVIDFTIVRNDDKIEFIINDYGKGIPQEVQDKLFKEMVTTKGKG
                     GNGLGLYMSYSSIKGRFGGNMWFVSNEGKGTAFYISLPFIRNGAA"
     gene            complement(17002..20952)
                     /locus_tag="PK733_03080"
     CDS             complement(17002..20952)
                     /locus_tag="PK733_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOJ09557.1"
                     /translation="MLDKINRCPLSYPQKGIWYIEKLYPDTSIGNIAATLKISGNIQF
                     DVLDKAINKFIKMNDSMRIRVLEHNGEPEQYISEYRYCKLDYLDFSSGDMEELYTWDT
                     KITRTPFFKLDSPLFYFAMIKINDNSGGFYVKLHHLIGDAWTLVNLGNEIVENYKTIS
                     NGNEIIIENKPSYLEFLLSEQQYLQSERFYKDEEYWVDKFDTLPELTILKDRKSYNKS
                     IKASRKTFVLPGKLSAKIRKHCSEYKTSIFALFLGAMAIYINRTRGKDEVIFGTPVLN
                     RSNKREKETMGMFISTVPIRIKIDDNLDYTSFAEGISKEWMSILRHQRYSYTLLLKKL
                     RQKHKDIDKLYDIVISYQNAKVSKKENVNEREGRWHFNGYQTESLCLHINDREDDGNI
                     VLDYDYLSDLFYAKEIEYIHDHMTRLLWHALDNPARKLPYIEMISEKEKQKILYDFNN
                     TNAEYPRDKTIHQLFEEQVEKTPDAVAVVFDDREMTYRELNENANKLAGVLRAKGVKP
                     NEIVGLMVNRSLEMIVGIFAILKAGGAYLPIDPEYPEERIRYMLGDSNARILLTQKSF
                     NADLYYRGQLIELDNLMDNTGFLMDNFGLIKDDASSELSNLENVNKPCDLIYIIYTSG
                     STGKPKGAMIEHRNVVRLLFNDKFQFDFNSKDVWTMFHSFCFDFSVWEMYGALLYGGK
                     LIIISKGATQDTKEFVNILKKEKVTVLNQTPAAFYNLINEEIQCSENSLALRYVVFGG
                     EALKPVMLKPFKEKYPDTRLINMYGITETTVHVTFKELGEEDISKNISNVGKPIPTLK
                     TYIVDKYLNLLPIGIPGELCVSGDGVGRGYLNKESLTKEKFVPNPFCANEVMYRSGDL
                     ARLFAQGDIEYLGRIDNQVKIRGHRIELGEIESQMLKYGEIKEAVVIARNNKLGNKQL
                     CAYYVSESSFKTSELREFLLKYLPDYMIPTYFIRVNKMPLTSNGKIDKKNLPQPEEKF
                     HSEDEYAAPQNKIQESLVQIWSQVLGVGEIGINDNFFHMGGDSLSAISVVSKIGGDIT
                     FSDLYNNPTIKMLSEALTNKDKESLTNKILIKMAASNLETSNNIICFPYGGGNAAIYK
                     DLADSVLKISEDYSLYAVNLPGHDIGCNEGLLPNHETALRLLKEIKETITGDIILYGH
                     CVGSALTIETAKLLQDQNIRVKAILIGGILPPGKTNIPGENYDPWRLVSDKNIIRFLN
                     RIGLPKFDLENEYTSILIKSFRHDVRNYYKFFYEFGTKYREKLDNPIYFIAGEKDLIT
                     KGYKSRYKNWGKYSKHVELITIEGANHYFIKTHSSRLAEIIIQISREAVLMEVL"
     gene            complement(20987..22306)
                     /locus_tag="PK733_03085"
     CDS             complement(20987..22306)
                     /locus_tag="PK733_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOJ09558.1"
                     /translation="MPQYLAIFCFFISDFIQIMFEGAAIITVLNRKSSITRLAMFGLI
                     NALTISIPFLINNTQYFFLMRIAIFAITMILTMKLILHLDILTSVISLIFIIIINTIA
                     EYICMVILKLCLTEGFLTDLMKQVTIFDFILKSILCLMVVIVTAIIYYFKNIIAIPEN
                     IKITRMIGLALNFIIMFMLIAPNMLFILNNFGNIKLGLIVYNLILLFLLTLINVYNSK
                     KFAELEMQKQKIDFQNQYIDTLSEAVDGLRSFKHEFNNLVNVIGGYIALDDFNGLKKY
                     FEQLKSKCTKINNIFPLNSYAKNDPAIYGLLLSKISTAEIKDINFNVNLTTELYSKAM
                     KSFDLYKILGILLDNAFEAAERSEMKYVEFFTRKLDNDGNLLLEIKNSFSGNIDVNMI
                     YTNGYSTKSGHSGFGLWEVRKTISRYKNFSINTMVNHNIFTQQIKLY"
     gene            22588..23274
                     /gene="deoC"
                     /locus_tag="PK733_03090"
     CDS             22588..23274
                     /gene="deoC"
                     /locus_tag="PK733_03090"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOJ09559.1"
                     /translation="MLPERQIEIPHKKKIASLIDHTLLKQNASSRQVEDFCRQAAKYP
                     FATVCINPCHVKIALEELKGSDIKVCTVAGFPLGASADGVKAFEAGKATDDGAQEVDM
                     VINIGALKDGNYVYVEKDIKSVVQAVKGLIVKVIIETQFLNEQEKEMACKIAQNAGAH
                     FVKTSTGMFGGGATVEDVALLRSVVGTGMGVKAAGGVRTLKDILMMVEAGANRIGTSS
                     AITIMQELGK"
     gene            23452..24207
                     /locus_tag="PK733_03095"
     CDS             23452..24207
                     /locus_tag="PK733_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOJ09560.1"
                     /translation="MTKLISWNVNGLRAIMGKGFLDFFKEVDADIFCVQETKLQDGQL
                     QLELDGYCQYWNYAIKKGYSGTAVFTKIKPLDATYGIGMEQHDCEGRVITLEYSNYYL
                     VNVYTPNSQRGLERLNYRMTWEDDFRSYLKQLDSKKPVVVCGDMNVAHKEIDLKNPSS
                     NKNNAGFTQEERDKFTELLNAGFIDTYRYFYPDRMDAYTWWSYMFKAREKDIGWRIDY
                     FCVSERLKDNLCKADIHSDIMGSDHCPVELVID"
     gene            complement(24797..27379)
                     /locus_tag="PK733_03100"
     CDS             complement(24797..27379)
                     /locus_tag="PK733_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ09561.1"
                     /translation="MREWIVSSSVLILIIIALRHVLKGKIDLRLQYALWVIVLLRLVI
                     PVSFVESSVSVMNIFQTYDQNAGIQNETLKNIPTVMVNLPTNVKHVNNSLISKSTATY
                     NENTASVSYNGLFNWEQIAKIIWLIGIGAIGLTLIISNIYFAYKLKEIRYKTDIVGYP
                     LPIYGTDEVETPCMFGLFCPVIYVTTQVLDNETVLRYVLEHEITHWRHKDHIWSLLRC
                     MCLALHWYNPLVWIAAAYSVQDAELSCDETTLQRMGEDSRVEYGRTLIGLTCTKRNVG
                     SLLSATTTMTGSKRSIKERIELVAKKNKMATYTLIAVLLIAAVAVGCTFTGAKDNKNK
                     IVPLTEKEVQRYNKIFEPLIFDEQGNDSPNPLSHFFTSYYDKPEDIDLPNFLRYFPPD
                     EDVTDETEFKALKEAKNWPFGDDIALKRMPVPIHKFSAKTVNEALQKNMGITLDDLSG
                     VGTKDLIYIKEYDAYYNFTSDFAAGIFYCTHGETQGDIVRLFNEFVTLTLKKQGDDYL
                     FISHQRIGEGTDANSATKDLGVAVSIKPRGYIPEAVLNYAVEFVTQQVDYYNNEGKNL
                     PSGRGTFAITKAKVTALTKINTGTASLTTDIQMWLLEYRLCPDNPGNVVLAGGMKMED
                     VDGESWITEWGSTGQPYLVLAHDIGMDIWQRICVTNSEVIMTDYGKPEMLERYGNEYT
                     AASLELYRKEAAEQNNEINQKDILQYIEEGIYIPGDAKEYLEKKEFNNPGLLINNELQ
                     PIFDEYKETFDDMLLKGLQPIDILRLRNKAVEEENLEVEINLIILPPEINREDFLKEV
                     QNDEVSKQNNKLLLEKYRQFNGKVIEVIINEGKAYVVVQGNGFWRFEKTENGVWKFGW
                     LSRQ"
     gene            complement(27376..27762)
                     /locus_tag="PK733_03105"
     CDS             complement(27376..27762)
                     /locus_tag="PK733_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ09562.1"
                     /translation="MTENNINLTPTEWNLMECLWESGPCTGREATEYLSEHIGWARST
                     TLTLLRRMTEKGIIKCEEIDGIKSYSPLVKREDAVMHETNSFLSRVYKGSVSMMMSTI
                     AKKQELSRVEIEELYTILRQAEEANK"
     gene            28023..28340
                     /locus_tag="PK733_03110"
     CDS             28023..28340
                     /locus_tag="PK733_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09563.1"
                     /translation="MLKVILVEDEKILLEDLRDNFYWASAGIEITETARNGREALEKI
                     KANKSQVVVTDIKMPVKAGIWDMVDGKHVPKQEITDLYAKDYATSLKQAGTRKYAFIF
                     KPD"
     gene            complement(28781..29275)
                     /locus_tag="PK733_03115"
     CDS             complement(28781..29275)
                     /locus_tag="PK733_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ09564.1"
                     /translation="MNYELDLAIVEGKVNDASINSLLLGTDYLVLVVPNNHRLAKKSM
                     VTINDIKKEKMILRLPNSGTRNLFIAHLESLNMSLAEFNVILEVDNIATIKDLIRRYY
                     GVSIFARSACLDELSKGKITVLPIEKLSMVRETSILYHQGFNHTDFLQDNTKLYNEIA
                     RIYK"
     gene            29367..29927
                     /locus_tag="PK733_03120"
     CDS             29367..29927
                     /locus_tag="PK733_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ09565.1"
                     /translation="MNKLITSQEAILRAGKEIVLEKGLQGLNIRDVARRCGVSVGSIY
                     NYFPTKSDLMVATIESVWKEIMHGYKICEEQHSFVDSIQSLFLNIQKGSEKYPSFFSA
                     HSMSVATLDKEKGRESMDKYFSHMKKGLLATLNADSGVMGDAFSPAFNKEDFIDFIFS
                     NLMTLLMEQASSCDFLLEVIRRIIYK"
     gene            30023..30700
                     /locus_tag="PK733_03125"
     CDS             30023..30700
                     /locus_tag="PK733_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09566.1"
                     /translation="MQAIMETLFDVVYLTTVVTLGVLMIKNNNGNKQCTLFGIMAVVL
                     GLGDSFHLVPRAIALCTTGLENYTVSLGIGKFITSITMTVFYILLYYVWRKRYDISGK
                     QDVTIAVYLLAAIRIILCFFPQNAWTSANPSLSWGIYRNIPFALMGLLIIVLFYKSTK
                     EHNDKSFRFMWLTIVLSFAFYVPVVLWADVSPLVGMLMIPKTCAYVWTVWIGYSDMRN
                     NWKGVDA"
     gene            30700..32106
                     /locus_tag="PK733_03130"
     CDS             30700..32106
                     /locus_tag="PK733_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ09567.1"
                     /translation="MQEQDKRIQIMQDAPPLKAIFTMSIPVILGMMVNVIYNLVDTWF
                     IGMMGDELQLAASSYCTPIFVIVMAVASLIGTGGASYLSRSLGAGRKDKAEKTLATGL
                     LLVCLFGLLVAIAGLVFRSQIPHILGASELSFKFTQQYGTILMLGGIFIIGNFALGQL
                     IRAEGSTKLSMIGMMVGAVANIVLDPLFIFTFGWGISGAAVATVIGNGLSVLFYLVCY
                     LRRKTLLRLTFRGFTMEPEILKEIFVIGIPATIGQMLISIAQMTCNNLASAYGDVTVA
                     SLGIALKIMTIGTYVFMGFSAGCQPLMGYNYGSGNYERMTEFIKTGILSTSLVGLFLT
                     LVLGASSPFLVAVFTPLEEVRSSGSAILRTLILSLPFMGGITLCSTTFQAMGKPLKAF
                     ILTISRQGLLYIPLLFLLDHLFGLSGMVYSQPITDLIMLLVASAFILKVLHSLKTGLR
                     ETDNLKDMKGEIDYEKIY"
     gene            32090..32362
                     /locus_tag="PK733_03135"
     CDS             32090..32362
                     /locus_tag="PK733_03135"
                     /inference="COORDINATES: protein motif:HMM:NF022521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HOJ09568.1"
                     /translation="MKKYINISFIYAMTAIACGVFYREFTKFLGFSGKTTLAFTHLHF
                     FVLGTIMFLLIAIFSSITNLSQQKQFSRFMLLYNIETSKGEKENGS"
     gene            32374..32781
                     /locus_tag="PK733_03140"
     CDS             32374..32781
                     /locus_tag="PK733_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOJ09569.1"
                     /translation="MNLEDRFFYIQSIIDVYDREIIDYHIGLRCEGEDAAATLVRALM
                     RRNLFESDVKPVIRSDNGPQFTSNKFEDKCLEVNVEHERIPCKTPNKNAHIESFHRIF
                     EDECLGHREFESYEDTYKKVLHLFISSLYNPRE"
BASE COUNT        10253 a         6360 c         5548 g        10891 t
ORIGIN      
        1 atgtgtttgc catttgctat accaccacag ccaattatac caatcttcag cttttgttcc
       61 tttttacaac aatccttgca acaatttgac atacaataac cccctgtaat tttaataata
      121 aagtactgct tatatataac gcacaaaaca aattatatta tcagattgct aattatatat
      181 caggttttat gcgcttttgt tcccataata aatgtttaaa aataattcgc cctttctcat
      241 gccattggct ttaatcataa tgttttatat cataattatg aaggctttcc tgtagattcc
      301 cttattataa gctcgtagtc caggaatata ctcctgggtt caataacctc gccgcgcatc
      361 tgcataagca aaagctccat tgcttcgcat cctaattcaa actgcggctg agatatggtt
      421 gtcagcatag gttcacacat tgaagcataa gttatgtcat caaagcctat aatggaaata
      481 tcttcaggca ccttcaggcc tttttccctg aagcatctta ttgctcctat agccatactg
      541 tcggatatgg cgaaaactgc cgtaggtata ttgtcctttt tgagtaaaaa ctgccttcca
      601 gcgcgaattc cgcttttgaa gctgtagttg ccctgtttta tcaggctggt atcatataca
      661 aggcctacat cctcaatagc ctttttataa cctgcattcc tttgaatggt agaaataaaa
      721 ttattattgc atgtaatcat tccaatcttt ttgtggccca gccctattag gtattgtaca
      781 gctctatatg ctgcagaaaa gttatctatt gaaacatgag atacctgggc atcctcttta
      841 tactcacagc actgaactac agagaagctc ctgcctatgg ccgataattc ctcccgatcc
      901 agctctggag ccataaaaat cacgccatcg gaaagcctgc ttttaagcag cttaagatat
      961 gctttttctg tttccagctg tgaatctgtg ttgcataata aaatattgta accgttttca
     1021 cgtgcgacag attcaatacc ttttacaact tttgaataaa acggattgga tatgctcggc
     1081 agtaatacca atatcaatct ggtttcagtc cgtctcagat ttctgccaag gagattaggc
     1141 ctgtaatcca gttcttctat tgcctgcatt acattatttc gtgctttttc agatactgca
     1201 gggctgttgt taattaccct tgaaactgta gcaacagata cccctgcttt ttgtgctaca
     1261 tcttgaatag tcgttggcat atttccctcc tgttttgtaa cggattacat ttcttattat
     1321 aaaacacatt ttggaaatat tcaataggaa tgttatatgg aaatatccgt ccccttgttt
     1381 ttggaaaaac ctgttttata aaatgtcacc attaccacaa gagcgccggt tataaatgaa
     1441 actaaataca aaagctgaaa attgcttaaa gggaaagcaa gaccgctata gtccggcatc
     1501 acattaatga gaagtttacc aataataggc gccagcagaa cacttgctcc gtaaattgct
     1561 ttaaaccatc cttcatatat ggatttggca ctttccggca ttatctcaaa tctatatgcc
     1621 attatggcaa tattaaatcc gccattgcca aagcctccaa taagtgcaga aggaagtaac
     1681 atgaagtagg tttttctggt taaaaataca tacattagaa aatcaattat ggtgaagaaa
     1741 gcagaatatt taagaactga gattgtaccc tttctgctct gaatccgtcc ccaaaacttt
     1801 gtacttatta tcattacaat atatgtaatg acatttattg tagaaataaa tccataatca
     1861 aaatttaaat accttattaa atatattgaa gtaaaggaag tagaaattgt aaggcacaaa
     1921 taatatgcaa atataaaaat aaggaaatcc ctgaattcat tgctttttat aggttcgaga
     1981 aatatggttt ttttactttt gttttcctcc ggtattttat attccggctc ttcaataaat
     2041 aaaagtatta ccgtatcaat tactgataat acaaggctgc tgacaaaaat tattaaaaaa
     2101 cctgtatagc tctttttata aaaatcaaga acataaccca ttacaagagt agttatagta
     2161 aaggcaattc ttaacaaaaa cattctgaag taaacataat catttttttt accttcaggc
     2221 gctatctcca tataccagat catccaccct aattgataaa aaccccaaag caggtcagca
     2281 gtaataacca ttactgctac taaagtgata atgagttttt catctttgga aattaaaggt
     2341 aaaaaaacta ttgagcatgc taaaaaccgt gaaagagcgc ctactgtaat aagtagtttt
     2401 ttacgtcttt ttatcctctc aaatatgaga aaagaaaata cagtaaggat agaagcccat
     2461 gttcccgcat tattaagaat ggctacaata aaatcagagc ctttcaaatt tattataaac
     2521 ccggataaga acactccgct ggttaaaacg taagcggcat ttatcaatgc cgcatctata
     2581 agaaacagaa tctgactgtt tttgtatttt ccaccacaga ttcttttatg tccaaaaagc
     2641 tgtttgccta aattttttat ggtaatcgcc ctttctaaaa ttaatagcaa aaagaaaagt
     2701 cagcatcgtt aatatcataa ttacattatg acagataata tgtttttatg tcaaacatta
     2761 ttaaaaatgg gggggcatat tttatacttt gatttatggg gaatatattg tataatagca
     2821 ttattctttg tttttttgag gcagataact gtgtaaactg cttattaatc tttaattaat
     2881 gagatctaat gcaataataa aaaatttgac tgttataatt tataggggtg tgagaatgga
     2941 acaattaatg aacattacat taggaaatct acttgatgaa atagcggaaa aatatccgga
     3001 ccgggatgct gttatatata cagacaggcc cttcaggaaa acatattcac aattcaggga
     3061 gctttgcaac caggttgcaa aaggattcat ggctttggga gtcaaaaaag gcgatcatgt
     3121 tgccgtatgg gctacaaatt atcctgaatg gatgctggca caatttgcta cagctaaaat
     3181 gggagcagtc cttgttacag taaataccaa ctataaaatt tttgagctgg aatacctttt
     3241 aagacagtct gattccacca cgcttatttt gattgatggt ttcagagatg caaaatatat
     3301 tgatataata aatgaactat gtccgcagct aaagaattca gaaccaggaa agcttgattg
     3361 tccttctctt ccatttttaa aaaatgttat ttatattggt gataaaaaat attctggtat
     3421 gcttaactgg aatgatctgt ataaaatggg ccgggatatt tccgatgcag agcttgctgc
     3481 aatacaggaa agtcttgata tacatgatgt tataaatatg caatacacat ccggcactac
     3541 cggctttcca aaaggtgtaa tgcttactca ttataacatt attaataatg gtaaatccat
     3601 tggagactgc atgcatttaa cccatgaaga cagactatgc atcccggtac cttttttcca
     3661 ttgttttggg tgtgttctgg gcataatggc atgtgtaaca catggaaccg ccatggttcc
     3721 catagaatat tttgaacctt taaaggttat gcaggctgta caggatgaag aatgtacagc
     3781 tttacacgga gttcctacaa tgtttatagc catccttgag catcctgatt ttggcaaatt
     3841 caaattcccg aaactcagga cgggtattat ggcaggttct ccatgtccca ttaaggttat
     3901 gcagaaagtg gtagatttaa tgggggcctc ggaaataaca attgcttatg gacaaactga
     3961 agcatctccg gtatgtacac aaacaagagt taatgatagt atagaattaa gagtatctac
     4021 agtgggcaaa gccctcccat ttattgaaac taaagttgta gatcctgcaa caaacgaaga
     4081 tgttcctcca ggtattgcgg gtgaacttgt agcaaggggc tacaacgtaa tgaaaggata
     4141 ctataaaatg cctgaagcta cggctcaggc tatagattca gatggatggc ttcatactgg
     4201 ggacttggca gttatggatg aaaaagggta ctttaaaata accggaagaa taaaagatat
     4261 gattatcaga ggcggagaaa acatatatcc caaagagata gaagagtttt tatatactca
     4321 tccttcagta aaagatgtac aggttattgg agttcctgat aaaaagtacg gagaagttat
     4381 attggcttgt attattctaa aggaaggcct tactgctaca gaagaagaaa taaaacaata
     4441 tgtaagagat catatggcaa ggcataaaac acccaagtat gttaaattcg tagatagttt
     4501 tccgctaacc gcaagcggta aagtacaaaa gtataaaatg agagaatggg ctattgatga
     4561 attaggactt cgggatgcag cctctataga aacagcataa tgtaatgaaa cagtataata
     4621 caatagtaat agcataacta atggagactg cataaaggaa gcatcaggtt taggtatgca
     4681 tatccaataa attattcgga ggggatatat ttgaaaatat cattttctac actaggatgt
     4741 ccaggatgga cctggaatga aattgttagt atggctaaag attttggata tgatggtatt
     4801 gaagtaaggg gcatcaaaaa tgaactatat gtaccaaaag ttaaggcttt tctaccggag
     4861 agcattgagg caacaaaaac ccggctgaaa actcttggtc ttggaatttc atgtcttact
     4921 tctgcttgta atttatcgga taaaacaaat attgatacta atataaagga aggtaaagag
     4981 tatattgatc ttgcaagaaa attggaggtt ccctttgtaa gaattctggg agacccaaac
     5041 cctcacccgg gagaaaacat tgatttcaat tttactgtgg aaagcctgac aatacttgca
     5101 gagtatgccg ggaacaatga agtaaaggtg cttgttgaaa ccaatggaac ttatgcaaat
     5161 tcagaaacaa tattaaaatt ggtaaccaca gttaataatt ctcatgttgg cgttttatgg
     5221 gatattcatc atccataccg tttttttaat gagccgatta cgaaaacata tgaaaccctt
     5281 aaagattata taaaatttat tcatgtaaaa gattcggtat tggtgaacaa tagtgttaag
     5341 tatagaatga tgggttatgg tgatgtaccg gtcaaagagg ctctccttat tcttaaagac
     5401 gaaggatata agggatatgt ttccctggaa tgggttaagc gctggaacaa ggatcttgaa
     5461 gaaccgggag ttgttttctc tcattacatt aactatgtaa aaaatataat taagtgattt
     5521 aatttcgatt cagcagttta atgaaatcgg ataagccagg cttttctact atgaagataa
     5581 agtaattaac cgctgctctc cagtattcta gggggtgcat ttaatttata tgaaaaaaat
     5641 actggtaagg agcatcataa cgatagcccc tggtaaatat ttcttcatat ttgataaatg
     5701 caccatattc catatttctc actgcttaat cctcttcacc aactcctcag gaagctttgg
     5761 agtcccccct tcccgggtgc atacataact tcctattcta tttgccaggc agtttatttc
     5821 atcaagatca agctttttta aaaatcccat tataacagct gctgtaaagg catcccctgc
     5881 cccaaccgta tcaacaatat ctgccgggaa acctgggtga tccgacacac gtccttctga
     5941 taaaagaaga ctgcctttac tgccccgggt caatgctatt agttttaggg agtatttttc
     6001 tgccaatttc cataagatgt tcaaggtgct gtttttatct tcttcacata tctcatgtcc
     6061 caagcctaaa agttttgaaa caacaggcag ttcttcatca tttaatttga atatgtctgc
     6121 atgtttcagt gaattatgta taatttcttt attgtaaaaa tgctgcctta aatttatatc
     6181 gaaaactctt atacattggc tttctgcatt ttcaacattt tccgggtcct ctgcctttga
     6241 tgcttctcgt gcattctcta acattttaag tattgtattt cttgatgttt cagacctttg
     6301 tccaagggta ccaaaacata ttgcatctgc tttcccaagc agctcagatt cctctttttc
     6361 agaataaata taatcccacg caacattttc atgaataaaa tatgaaggta tgcccctatt
     6421 gtcaagtttc acttcaactg tacctgtagg gtggttatta ttaatttgaa tatagcggct
     6481 gctcaaaccc ataatgttta ttttgttaat tatttccctc cccagattat catttccaac
     6541 tgtgcttact atataagcat cagcaccaag agctgaagcg tggtacacga agttggccgg
     6601 agcgcctccc aattgtttcc cactcggaag catatcccac agtatttcac caacccctgc
     6661 tattgtatat ttcttttctg aacttttctt atctgacata gaccgcctat cactccattc
     6721 atcacaattt aagcctatat tacaacaaat tcaatacccc agtataggaa ttatccaggt
     6781 attttattaa ttcctatact tcaagaagtt acgagtttac atgacacagt tcatattcat
     6841 caatgttgct tttatctgtg ttttccaaga tactttcaag agcccttgcc agctggtcag
     6901 ggcatgatgt tcccttacct ccacagttaa ttcctttaag ctttcttatt acctcttcta
     6961 taggcatgcc ttccacgagt ttgcttattc cttgaaggtt gccgctgcat ccggacttga
     7021 agctaacact aaccaatgta ttcttttcat agcaaatatc aatttgcctt gagcatgtcc
     7081 cttttgttat gtatgtaatt tgctgcattt aaaataaccc ctttaccttt tcaattacct
     7141 tttgataaaa ctatgcaata gcaatagtat acaatatatg atatatgatg attataatca
     7201 taattgaccg ggaatttcaa ccaccatatt aataacctat tgtttatgca gccttaagct
     7261 acctgagagt tcgtcaccat ttttcggtta agtgcctcca atatctcaca ggcaaaagcc
     7321 tcttatgtaa atccaggtta tatctttccc ctatggtcac tgcgttataa aactccttcc
     7381 ataaatcacg gtaaaacttc tcatttgtat ctattcccgg aattcgaccg ggatttgcat
     7441 ccaccagcat ccactccctg caattatata aagctgccaa ttctctggat ttatcgtgaa
     7501 tcaaccagtt ctggtcagcc atacgttctg caaagtgtgg tgctaacagc ggcagaatat
     7561 tgttatccgg ttctattgga gcataataaa tattcccctc aagcagactg aaccgtaaaa
     7621 atcccatcat tctatgcact tcaagcccca ctctctgcct tatttggtga accctcagta
     7681 ctctgtcgtc tgacaggtat aaatcaacgt ttttccccac tttccagcct aattttaaat
     7741 attcatatat aattgtgctg ttttccttat ctttatcggc cagaaaaaca tgataaatat
     7801 tgtcaagggc atcttgagaa atcttatgta ttatggaatt atatactctt tgagatttat
     7861 caaaatcagt actaatatgt acatacttaa acataaaatt tgtttgaaga gcatcttcag
     7921 attcaatttt ctcggggtat tcttttcggt aatatgcttc ataaacagcc gaaagaagac
     7981 cttcaaaact cccgtcatat gtatagcaca gtatataatc acctccaata atgcttatat
     8041 tatctctttt accggatcaa taccgattaa cccttttact tgtacagctt catttgcccc
     8101 aattaactct gttgacccag ctacaccaac ccaattttca ttagcagacg aaaagagtga
     8161 aagctgcctg taaccttcat caaatgcccc aggaagttta agaccatctg caataagatt
     8221 tgcccgtata aaagtatctt tcatttcagc tctctgatag tattttccct tgcatgtaat
     8281 aaagtattgt gcccttttca gtaccacgcc aagttttttt aaatcatcaa aatccagcga
     8341 accaaccctt cttgctgtaa ttatcctttt cgcagatttt actccaatcc caggtacacg
     8401 caataacatt tcatacctgt ctctattcgc ttctacagga aatagctgta aatttcttaa
     8461 agcccagtca catttcggat ctaatacagc attaaaattg ggatgctcct gagaaagcaa
     8521 ttcatctgca gtaaaaccgt aaaatcttaa cagccagtca gcttgataca atctatgctc
     8581 tcttaaaaga ggaggttttt ttattgatgg aagagcaggg tgatttgaaa caggcacata
     8641 cgcagaataa aaaactcttt ttaaaccata ccgcctatat aaaccttcag aaagtttgag
     8701 aatgttaata tcattatcag gtgtggcacc tactattaat tgtgtgctct gaccggcagg
     8761 tacaaagtat ggagcttttc tgcacttttg aatgtcctcc ttattttctt caattctgga
     8821 ctttatataa cccataggcc ctaaaatgga ctccttattc ttctgaggag caagcaattg
     8881 caaccccgcg cttgtaggca gctcaatatt tacactcata cggtccacaa gctttcctgc
     8941 ttcatctata agcttataat ccgctccagg aatagcttta atatgtatgt acccgttaaa
     9001 cttgttttca catctaagaa gcctgactgt tttcaagaga agctccattg tataatccgg
     9061 atttttcaat attgccgaac tcaggaacaa gccttcaata taattcctgc gataaaagtt
     9121 aattgtcagg tccacaactt cctcaggagt aaaagttgcc ctgggaacat cgttcgtagc
     9181 cctgttaacg caatatgcgc aattataaac acaggcattt gtcaagagaa tttttagaag
     9241 agatatgcaa cggccatcag cagtaaaact atggcatatg cctcccgaaa acccagtacc
     9301 caaacctccc ggagtattgt tccttgtgct tccacttgac acgcaggata catcatactt
     9361 tgcactatca gcaagaatat ttaacttttg tataagctcc atccaagcac tcccttgcat
     9421 actattctct aatattataa ctctctgact atttaactat ggcaatattt catggcacta
     9481 ttttaccata taaataatga aattgcaaac atatgttcta gaaagggtgc atttttaaaa
     9541 gtgaagaaat atttaccagg agctgacgtt acactgctta gagcctttaa attcagctcc
     9601 ggacaaaatc gaactcataa aattgtctga atgaaaatta agagacaatt ttacaaaaaa
     9661 caacgatttt gcttatccta cgctccgatt aaaaggctct acatcagctt cacttatgct
     9721 ccttaccaat attttcttca ctttaattaa atgcacccaa agctaaattg aaatttacaa
     9781 ttatttacta attaaagctt gttcagatac tcttgaaaat cataaaaatc tttaaaacct
     9841 aactctttaa tattaaacat taatgttcct gtagctatat ttcttccacc tgaatgtaaa
     9901 tgaacaggga ttctcacata ctcgccatta atagggctaa tacctttcca tatatgtccc
     9961 ttttttgaca tctttttttc taaccccagt gacaaacata cctcgtcaaa ttctctaaaa
    10021 cttatagttg gcatccggat catgcatcct ttctttagtt tttgcgatcc aaccccaaca
    10081 cctttaacaa ctgattttta tctacacaac cttttaattt taaataataa gggtactgtt
    10141 ttttgtattt ttcaatccga atataaagct caatatcgtc aaaataatct tcagtaagat
    10201 ttacaatatt atctaataaa acatcaatgg catcctgttt tgtctcacca aagcctgatg
    10261 cacctatttc aggaactaaa acttcgtatt gttgtgtagg attatcataa cttattaaag
    10321 gattaaactg gtaacagcct aatagaaggt ctagcagttc tgttgaaatt attgttgctg
    10381 ttttccctcc tttcttcaca ttaccggtaa caacttcctg aaatcgatct atagcattat
    10441 ccataacact tgatagatcc tttctgagat ctgctactcc cattatatct ctgctaaaca
    10501 actcgttaga aacacggatc attttatcca cctccttacc tggattaggc tctgaatctt
    10561 tattaaatac tttcttcatt ttgctgcctc cataataata cattatgtac ataaagtcaa
    10621 tatatatcat tatgtaatat atgcagaatt aataacaata tgcattcgat ttcaaataca
    10681 aggctctcat ttgtgtataa taatagacca aatgcttatt ttgttgcagc catattcacc
    10741 catatggtat aatattcaca aatcaataaa cagctgatat gtaatccgga attagtcaat
    10801 tattactcaa tcagcagtta tttcactata agtttatttg tttatatgcc atttataagc
    10861 cagtataagt caaaaggatg tgaatttatg atctgtgaaa atactaagtg cattgcatac
    10921 ataggtacgt atacatcagg caaaagtgaa gggatatata tattcgaaat ggatacccaa
    10981 acaggtgaat tgaaacctgc gggagtatgc aagggtttgg aaaacccttc atatcaggtt
    11041 atagataata agacagaata tttatatacc gttatggaaa ctgaaaagtt caatggtgag
    11101 aaaggtggag ctgttgccgc ttttgcaata gataaaaaaa caggtctgct ggaatttgta
    11161 aattgccagc ctacaaaggg cagagtccct tgccatataa gcattgatag ttccagagag
    11221 tatatttttg cagccaacta cactgaaggt acaatatccg tttttcctat cagtcaggac
    11281 ggaagcatat ctcctgtttc atgcatcgtt aagcatgagg gctcgggtcc aaataaggaa
    11341 aggcaggaaa aagcacatgc ccactatgta tctttaactc ctgatgagaa atatttgtgt
    11401 gctgtagatc ttggaattga tagaataatg gtatatgatt ttgacagaga aaaaggtcgc
    11461 ctttatcctt ccaagcatcc ggaggttatt atcaaaccag cttcaggccc acggcacatg
    11521 gctttccatc catctggaaa gtttgcttac ctgataaatg aattaagctc tgacattgca
    11581 gctttcgaat attcatcaag tgatggtact tttagtgaaa tacagtatat ctcaactctg
    11641 cctggcgaat caaacctgcc tcaggaactt agtaagcctt tagttaacta ttgctctgca
    11701 atacatatat cacaggatgg gcaattcctt tatggttcaa atcgaggtca tgacagtatt
    11761 gccatattta agatagatca gttatccggc aagcttgaat taacttccat aacatcaaca
    11821 gggggaaatt tcccaagaga ttttgcaata gatccctcgg ggaaattcct ttatgctgca
    11881 aaccagaatt cggatacaat tgttgttttt cggataacac aggaatcagg cataattgag
    11941 cctaccggca gcataattaa tgtaccagat cctgtatgta tcaaatttgt aatcttaaag
    12001 tagtaacaaa agatatggga taggagtaag aagatattgc ttcttactcc tcatttctca
    12061 tttccaattc cctagcttat tccaagcagt aatcttgcaa tagagaagta agacatcagt
    12121 gccagtgcat ctactattgt tgtaataaga ggacttgcca tgatagcagg atccaggttg
    12181 agtttttttg caattacagg cagtgctcct ccgataacct ttgcagcaat aactgttact
    12241 aaaagactta tgcatactgt aagagctaca taaaaatccg ttttttcaag aaaatatagc
    12301 cgtagaaaat taacccctgc cagtgttagc cctacaacaa ttgctacgcg aagctctttc
    12361 cagattattt tcataatatc accaaattca atctcaccta aagccagtcc tcttatgacc
    12421 aatgttgatg attgtgagcc ggaatttccg cctgtatcca tcagcattgg aataaaagcc
    12481 gccaatgata ccattgactg aagaagccct tgaaattttt ttattatatt ccctgtaaaa
    12541 gtagcagaaa tcataagtac taaaagccat attattctat gtttggcatg atcgaaaaca
    12601 ctggttttta ggtattcttc ttccgaaggt gccatagcag ccattttgtg aaagtcttcc
    12661 gtattttctc tttctatgat atctaaaaca tcatcaatag taataattcc tacaaggcgg
    12721 ttttcattat ctacaacagg catagctaca aggtcatact ttttaaacag gtttgctact
    12781 tcctcctgat tatcatgagt atatacagaa ataacatttg tatccataat atctgcaata
    12841 attacattgc catcacttaa tatcaatttt ctaattggca gcgaaccctg taatttacgg
    12901 ttactgtcaa ttacatagca tacatcaata gtttccttgt ccagcccggt ctctcttacg
    12961 tgctccaatg ccattttaac tgtcatctct ttttttaagt cgataaactc aatggtcata
    13021 acacttccgg cagaattatc aggatatttt aaaaactgat tgatcaactt cctccgtgtt
    13081 tcactggtat tcttcagaac ttttttaact acattggcag gcatttcttc tataaggtcg
    13141 attgtgtcat caagaaacaa ttcatctatt atatttttta tttctttatc cgctattgat
    13201 tctattataa actgctgctg ctcataggac atatatgaaa aaacctcagc agctatatct
    13261 ttaggtagta gacgaaaaac catcagagaa ttgtcctctt ctaactcttc caaaagctgt
    13321 gcaatatcta ctacgttcat ttttataatt tcatttcgtg catcaaaaaa tttacccctg
    13381 gtaatgaggt cattgattaa ttcattcata gtgcagcccc cctgtgcaaa gagccgccta
    13441 actatacaag gtgatagtta cggctctact tattttgtct attttgtttt taagaataca
    13501 tccggtacat cgttggggta aaggaccaga atccataact atcaccacct ttaaactttc
    13561 taaatttttc tactttattt aattatacta cttcgcatgg aaaatatcaa cgactataaa
    13621 aatgctccca aagccatggt tgcatttatt catatagaga agctatctcc ctctccatat
    13681 cctcaaagtt cttaagctcc cttgtgaaaa catctacaac ttttttatca aactgtgttc
    13741 cggcacccaa tataagttgc tttacagctt catcaagctc aagcctggat ctgtaaagcc
    13801 tgtcggacat catggcatca aatgcatcag caatagaaat tattcttgaa aggaaaggga
    13861 tttcattccc ttttatgcct tccggatatc catagccgtc gatcctctca tggtggtatt
    13921 ttacaatggg tacaatctcc ttaaacatag aaactgccgc cagaatgcgt gcccccttta
    13981 atggatgttt tttaatttcc ttgtattccc gctcatttaa tttatcattt ttaaggagaa
    14041 tatcgtctgc agtgcttatt ttccctatat catgaaatat accacttatt cttagcagct
    14101 caagctcttc ctctgcaagc tttagtgcct tcccaattct tactgcatag taggaaactc
    14161 tgtcggaatg tcctcttgta tatatatcct ttgcatcaac agcctgccgc agtgcttcaa
    14221 tagtgtccat atacctgatc ctcagctgct catatgtcat attcagctct tcgtttttca
    14281 tatttacaag agagtgcaga aaagcattgt ttatggaaga tgccgcctga ctcgcatata
    14341 tttccagaag ctttattccc tcttctatat catcactatc agcatatata acacctatag
    14401 cttctgaaaa ctcattaatc aaaggtagta tagctccatg ttccagtttt accacctttt
    14461 ggttcattct tgcgttgcca atatgttcta tcagatccgg agcaagcatt ttcataaaat
    14521 ccttcatttc tgccttatac tttcctatgc ctttaaatat actcttgtta gaacaaccgg
    14581 agtctatgtt ggtagtgtca tccacaagta taaaagcatt ttcactattt accagtggaa
    14641 ggatttcttt aagtatatct tccaggatat tcccaattga ctgtagctga tatatctttg
    14701 gaactgcctg cagtatcttg tttaagccat ctctgaattt ttttattgta cgcatctgag
    14761 atattgattt aattcccgat tcaataagca gaagcagctg gtcaaactta tcgctttttt
    14821 cacaatagcc ctggatttcc aattctttta tagtttctat tggcggagca aggtccttgt
    14881 gccctgttaa tagaagaata taaagctcat tattgaactc tctgattttt tcaacaactt
    14941 catcaccatg tataggatac atgagatagt caagtatgag aagatcaaaa tgctcatttc
    15001 ttactttttc tatagcttta acagggtctg tcaagcccgt aatattatac ccgcttcgtt
    15061 tcatcaacat tgacaatgaa tcaataatac cttcatcatc atcaacaatt aatattttga
    15121 aatcagaaaa taccttaatt ctcttttttc tcattacgat acctcctaag ctgctccgtt
    15181 ccttataaac ggtaagctta tataaaaagc agtgcccttg ccttcattcg atacaaacca
    15241 catattgcct ccaaaacgcc cttttattga agaataagac atatacagac caagaccgtt
    15301 gccaccttta cctttagtcg ttaccatttc cttaaaaagc ttatcttgaa cttcctgtgg
    15361 tattccttta ccataatcat ttattataaa ttcgatttta tcatcattcc taacaatggt
    15421 aaagtctatt actcctccat ttccttcata tgcatgaatg gcatttataa taatattgtc
    15481 aaatacctgg acaagattgt taacttcccc ttttatttca gtatacgggt ctacttttat
    15541 atcaatattg agtacacaat gagatttttt caattcatgt ttcattaaaa tgtcaatcct
    15601 tttgactaat tcctccaatg taaacttgat ggtagttgaa tctataagct taactgcctg
    15661 gcctttaact gttgaaatta catcagacat gtaactgcag tatggtttca tcttcaaaag
    15721 ccaatccagc atctctttag caatttcgtg atgatcctca aatgttacac ttttatcatc
    15781 tattgattcg tcatattcct ctgccagatc ttttaaagcc tctatacctc cggccaatga
    15841 cattattgga gtattaagat tatgcgctat accgccaata agttgtccta aagatgcaag
    15901 ccgctcctgc tccatcagga tttcatagtt tctttgaatg gtttcaaggt cctttttagc
    15961 ctgagaaata tcttttagaa ggattattgt gccaaggtag attccttggg taactattgg
    16021 tgttatttct attaaaaaat acttttgaaa tgacccaatt tttatgtttt tttcaaaact
    16081 aacgattgtt ctctcagcca cagctttttt tagtagattg aaaaggctgg aaatgttttt
    16141 atctgtagac ttatatatat ttataagatt tgtcaaatta tcttttcttt ttatttttag
    16201 tatattgcca aagttatctg taaaggtttt gttataatca attatataat aatctttatc
    16261 aataactata aacgaatcag agatatggtc tacaacgttt tgcaaagcaa ttggcacaac
    16321 atttaaaaaa tcaaatttaa agattgcaag aaagaaaaac aagaaagaga aaaagaaagc
    16381 aataacatta gtataaaaat atcccctgac tatctgaaaa gttagaagga aattatagcc
    16441 aaaggagatt attatcccta tagctataaa tatggattgt tttgagaaaa acccggcatt
    16501 ctttactgaa aaatatataa ggtatgccat acaagcagtt atacacagat acgaataaat
    16561 tgtgtggata ataaaatatt ttcctaatgc attagcatgt gtcagttcct tatattttac
    16621 ataattatag aaaagatgat ggtaatcatt cgtaaataat aatattattg aaattagggg
    16681 gattattaaa aataccccat atttccatga caatttgatt ttagtattag caaataccag
    16741 cccgaagaac aatgcagcaa cgggcgtcaa tataagtccc acatatgata tattaacgat
    16801 taaaacattg gtaggtttat taaacctgtg gtcatactct aaaataatag cacctaaagc
    16861 ccagataagc aatgtgccca gcaatgccaa aaatgcataa tgaatctgtt ttttaccttt
    16921 cattctcatg acaatataaa atatggcaag cataagaatt aatgacacat gtataagcac
    16981 aaaacccaaa tcagcaagca cttaaagcac ctccatcaac actgcttctc tgcttatttg
    17041 tataataatt tcagcaagtc tggaagaatg tgtttttata aaataatggt ttgccccttc
    17101 aattgtaatc aactcaacat gctttgaata tttgccccag tttttatacc tgcttttata
    17161 tcctttggta attaagtcct tttcacctgc aatgaaataa ataggattat ccaatttctc
    17221 ccgatatttg gtaccaaact cataaaagaa tttataatag ttccttacat catgtctaaa
    17281 ggatttaatc aagatacttg tatattcgtt ttcaagatca aattttggaa gaccaattct
    17341 attaagaaat ctgataatat ttttatctga taccagcctc caggggtcat aattttcacc
    17401 aggaatattt gtcttgcccg gtggaagtat cccccctatc aatatagcct ttactctaat
    17461 attttgatct tgaagaagct ttgcagtttc aattgtaagg gcgcttccta cacaatgccc
    17521 gtacaaaatt atgtcccctg taattgtttc ttttatttcc ttcagtaatc tcagtgcagt
    17581 ttcatgattt ggtaaaagtc cttcattaca tccaatatca tgcccgggca gatttactgc
    17641 atataaggaa taatcttccg atatttttaa taccgaatcg gctaaatctt tataaatagc
    17701 ggcattacca ccaccatacg ggaaacatat tatattatta cttgtttcaa ggttgcttgc
    17761 tgccattttt atcaaaattt tattagtcag agattcttta tctttattgg tcaatgcttc
    17821 agaaagcatt tttattgtag gattattata taggtcagaa aatgttatat ctccgcctat
    17881 ttttgaaaca acggaaatag cacttaatga gtctcctccc atatggaaaa agttgtcatt
    17941 aattccgatt tcaccaacac ctaaaacctg actccaaatt tgtaccaaag actcctgaat
    18001 tttgttttgc ggtgctgcat attcatcctc tgaatgaaat ttctcctccg gctgtggcaa
    18061 gttcttttta tcaattttgc cattagaagt aagaggcatt ttatttaccc ggataaagta
    18121 tgtaggaatc atataatcag gcaaatactt taataggaat tctctaagtt cgctggtttt
    18181 aaagctgctt tcagaaacat aatatgcaca taattgctta ttgcctaatt tattattcct
    18241 tgctataaca acagcttctt ttatttctcc atacttaagc atttgagatt caatttcgcc
    18301 aagctcaatt ctatgtcccc ttattttaac ctgattgtca attctcccca gatattctat
    18361 atcaccctgt gcaaataatc tggccaaatc tcctgaccgg tacataacct catttgcgca
    18421 aaatggattt gggacaaatt tttcttttgt taaagactct ttattaagat agcctcttcc
    18481 cactccatca ccactgacgc ataactcgcc cgggatacca attggcagaa gatttaaata
    18541 tttatcaact atatatgttt ttagtgttgg aataggtttt cccacattgc ttatattttt
    18601 ggaaatatcc tcttctccta attccttaaa agttacatgt actgttgttt cagttattcc
    18661 atacatatta attaatcttg tgtcaggata tttttcctta aatggtttaa gcattaccgg
    18721 ttttaaggct tcgcccccaa aaactacata tcttaatgca agtgaattct cagaacattg
    18781 aatttcttcg tttataaggt tatagaaagc tgctggcgtt tgattcaaaa ctgttacttt
    18841 ttcttttttc aaaatattca caaattcctt tgtatcttgt gtggctcctt ttgatataat
    18901 tatcagtttt ccaccatata ataaagcccc atacatttcc caaacagaaa aatcgaagca
    18961 aaaggaatga aacatggtcc atacatcctt actattaaaa tcaaattgaa atttatcatt
    19021 aaataaaagc cttacaacat tcctgtgttc aatcattgca ccttttggtt ttcctgtaga
    19081 cccggaagta taaattatat aaatcaagtc gcagggctta tttacatttt caagatttga
    19141 cagttcactt gatgcatcat ctttaattaa accgaaattg tccattaaaa aaccggtatt
    19201 gtccattaaa ttgtccaatt ctattaattg gcctctatag tacaaatcgg cattaaaaga
    19261 tttttgtgtc agcaaaatcc tggcattgct atccccaagc atatacctga tacgttcttc
    19321 aggatattca ggatctattg gcaggtaagc cccgccggct tttaatatag caaatatgcc
    19381 aactatcatc tccagtgagc ggtttaccat taatcctaca atctcattgg gctttacacc
    19441 ctttgccctt aaaactcctg caagtttatt agcattttca ttaagctctc tgtatgtcat
    19501 ctccctatca tcaaaaacta cagctactgc gtcaggcgtt ttttccacct gctcttcaaa
    19561 caactggtgt attgttttat ccctgggata ttcagcattt gtattattaa agtcatataa
    19621 tattttttgt ttttcctttt cagaaatcat ttcgatataa ggcagttttc tggctggatt
    19681 atccagagca tgccacaaaa gtcttgtcat atgatcgtga atgtattcaa tttcctttgc
    19741 ataaaaaaga tcactcaaat aatcataatc caatactata ttgccgtcat cttccctgtc
    19801 gttgatgtgc agacataaag attcggtttg ataaccatta aaatgccatc ttccttcccg
    19861 ctcattaaca ttttcctttt tgctaacttt agcattctgg tatgatatta caatatcata
    19921 tagtttatca atatctttat gtttttgtct tagtttttta agcagcaaag tataagaata
    19981 tctctgatgt ctgagaatcg acatccattc tttagatatc ccctcagcaa aagatgtata
    20041 gtcaagatta tcatctattt ttattcgaat gggcacagta cttataaaca tacccatagt
    20101 ttctttttct cttttgtttg atcggtttaa tacaggcgtt ccgaatatta cctcatcttt
    20161 cccccttgtt ctgtttatat atatggccat agcaccaagg aataatgcaa atatagaagt
    20221 cttgtattca ctgcaatgct tgcgtatttt tgcagaaagc ttcccgggca aaacaaaggt
    20281 ttttctgctg gcctttatgc ttttgttgta tgattttcta tctttgagta ttgttagttc
    20341 aggtaatgta tcaaatttat caacccaata ttcctcatcc ttgtaaaatc tctccgattg
    20401 caaatattgc tgctcactta ataaaaattc caggtaagaa ggtttgtttt ctataattat
    20461 ctcattacca ttacttatag ttttgtaatt ctctactatc tcattgccca gattaaccaa
    20521 ggtccaggca tctccaatca gatgatgcag cttaacatag aatccgcctg aattatcatt
    20581 aatttttatc attgcaaaat aaaacagggg cgaatccagc ttgaagaatg gtgtccgcgt
    20641 tatttttgta tcccatgtgt ataattcttc catgtctcct gaagaaaaat caaggtaatc
    20701 caatttgcag tatctgtact ctgaaatata ctgttccggt tctccattat gttctaaaac
    20761 ccggatacgc atggagtcat tcattttaat gaatttatta atagccttat caagtacatc
    20821 aaattgaatg ttccccgata tttttaaagt tgctgcgatg ttgccaatac ttgtatcagg
    20881 atacaatttt tctatatacc atatgccctt ttgtggatac gacaggggac atctgttaat
    20941 tttatccagc acaaataaca cctcccaaag tagtagtaga attatcttaa tacagcttaa
    21001 tctgctgagt aaatatgttg tgattaacca ttgtgttaat actaaaattt ttatacctgg
    21061 aaattgtttt tctaacttcc caaagcccaa atccgctatg tccacttttt gtggaatatc
    21121 cattggtata aatcatgtta acatcaatat ttcctgaaaa tgaatttttt atttctaata
    21181 atagattccc atcattgtcc agctttcttg taaaaaattc aacatatttc atctcacttc
    21241 tctctgcagc ttcaaatgca ttgtcaagta atatacccag tatcttgtac agatcaaagc
    21301 ttttcattgc ctttgaataa agctcagtag ttaaattcac attaaaatta atatctttta
    21361 tctctgctgt agatattttc gaaagcagca gtccataaat agcagggtca ttttttgcat
    21421 aagaatttaa aggaaaaata ttatttattt ttgtgcattt acttttaagc tgctcaaaat
    21481 acttctttag cccattaaaa tcatcaagag cgatatagcc gccaataaca tttacaagat
    21541 tattaaattc gtgtttaaaa cttcttaacc catctaccgc ttctgaaagt gtatcaatat
    21601 attgattttg gaaatcaatt ttttgttttt gcatctccag ttcagcaaat tttttagaat
    21661 tatatacatt tattagtgtc agcaaaaaca gcagaataag attatatact attaacccta
    21721 gtttaatatt tccaaagttg ttcaaaatga atagcatatt aggagcaatc agcatgaaca
    21781 taataatgaa atttaaagcc agaccaatca ttctcgttat tttaatattt tcaggaatag
    21841 ctattatgtt tttaaaataa taaatgattg ccgtaactat taccaccatc aaacaaagaa
    21901 tgcttttcaa tataaaatcg aaaatggtaa cttgtttcat taagtcagta agaaaccctt
    21961 ctgtgagaca tagttttaaa ataaccatac atatatattc ggcaatagtg tttataataa
    22021 taataaaaat cagactgata acacttgtta agatatctaa gtggagaatc agcttcattg
    22081 tcaatatcat tgtaatagca aatatagcaa tacgcattaa aaaaaagtat tgagtattat
    22141 tgattaggaa gggtattgat attgtcagag catttattaa cccaaacata gctagccttg
    22201 ttatacttga ctttctgttt aaaacagtaa taattgctgc cccctcaaac attatctgaa
    22261 taaaatcaga tataaaaaaa caaaatatag ctaaatactg cggcatattt atctccatta
    22321 caaatttatt aatctttata tctttttctt atggttataa tacttttgta tccaacttaa
    22381 tgtcgctata aatctctttt tgtaatttat attatacttc attcaataca ctctttcaat
    22441 aaaatagtct ccctatttgt gttatttatt ttagtttttt gcaatgtgct gtaacataac
    22501 tgctttatta acttttcgat atttgatata attctatatg tactttacag acagttataa
    22561 aacataattg atattggagg aacagaaatg ttaccagaga gacaaattga aataccccat
    22621 aaaaagaaaa tcgcatcatt aatagatcat acactactta aacagaatgc ttccagcaga
    22681 caggtagaag atttttgcag gcaggcggca aaatatcctt ttgctacagt gtgcataaat
    22741 ccatgccatg ttaaaattgc attggaagag ttgaagggtt cagacataaa agtttgtact
    22801 gtagcaggtt ttccactggg tgcatcagct gatggagtaa aggccttcga agctggaaag
    22861 gcaacagatg atggggcaca ggaagtagat atggttatta atattggtgc attaaaggat
    22921 ggaaattacg tttatgtgga gaaagacata aagagcgtag ttcaggctgt taaggggttg
    22981 atagtcaaag taattattga gacacaattt ctaaatgaac aggaaaaaga aatggcctgc
    23041 aaaattgctc aaaatgcagg tgcacacttt gttaaaacat ctacaggtat gttcggaggc
    23101 ggagcaactg ttgaagacgt agctttattg agaagtgttg tgggaaccgg catgggggta
    23161 aaagcagcag gtggtgtaag gactctgaaa gacatactta tgatggttga ggctggtgca
    23221 aacaggattg gcacaagttc tgccattacg ataatgcagg aacttggcaa ataactttag
    23281 gaaactattt tgttctttaa agttaactat aagttaaaag ttaaccataa gtttatttga
    23341 aatatagaac taaagctaaa gttttttaag ttataggttt taataaaact gagtacttat
    23401 aacttataaa tactacaatg gaaatatatt aagtaatttg tgagggtaat tatgacaaaa
    23461 ttaatatcat ggaatgtaaa tggccttaga gcaatcatgg gcaaaggatt cctggacttt
    23521 ttcaaggaag ttgatgcgga tatattctgt gttcaggaaa ccaagcttca ggatggacag
    23581 cttcaattgg agcttgatgg gtattgtcaa tattggaact atgcaatcaa gaaggggtat
    23641 tccggcactg ctgtatttac caaaatcaaa cctcttgatg caacctatgg tataggcatg
    23701 gaacagcatg attgcgaagg ccgtgtaata acgcttgagt atagcaacta ttatcttgta
    23761 aatgtataca cacccaattc tcaaagagga ttggaacgac ttaattacag aatgacctgg
    23821 gaagatgatt tcagaagcta tttaaagcag ctggactcta aaaaacctgt agttgtatgt
    23881 ggtgatatga atgtggccca taaggaaatt gaccttaaga atccctcttc aaacaaaaat
    23941 aatgcaggct ttacacaaga agaaagggac aagtttactg aattgctaaa cgcaggcttt
    24001 attgacacct acagatactt ttatcctgat agaatggatg cttacacatg gtggtcttat
    24061 atgtttaaag caagagaaaa ggacatcggc tggagaatcg actacttctg tgtttctgaa
    24121 agattaaagg ataacctgtg caaagctgat atacactctg atataatggg ttccgatcac
    24181 tgtccggttg agttggtgat tgattagcgg tttcatttga aaaaataatt ttatcagaac
    24241 ggcattgaat gagatacata gtgtcttcag cacatttcgt tgtaataatt cctttatttg
    24301 attcaataag cattgacagc tttgtagcat tgtcaatttt tgggacacct cgcttttgct
    24361 ttggtgcaac aataatatca cattgtggca ccaaagccag ttcacttttg aagaagtatt
    24421 ttcagaagtt aaatttgtca ttcctaaacc caatccttta cacaaaagtt cattgtcggc
    24481 cttttaaatc taaacctatc catggtccgg aactagatta ttggaattaa tacattgatc
    24541 cgatttagtc aacatattag gtgagggatc tgattccctt atattccttt actcgaccat
    24601 ggagtctttt attaatagtt ttaacctcca agtaattgat atatacacga catcaagaca
    24661 tttacccttt aataatgttc taccatcatt taaccagtaa acagtttcat ttacccctaa
    24721 aaaacaaatt cttcttcttt ttcttcaaaa gcattttaaa atatctgcag aatatcactt
    24781 atattatgct ctaaggttat tgtcttgata accatccaaa tttccaaact ccgttttcag
    24841 ttttctcgaa tcgccaaaac ccgttccctt gcacaacaac ataagctttg ccctcgttaa
    24901 taattacttc aataactttc ccattaaatt gcctatactt ttctaaaagt aatttattgt
    24961 tctgctttga aacttcatca ttttgtactt cttttaaaaa gtcttctctg ttaatttccg
    25021 gcggtaaaat aattagattt atttctacct caagattttc ttcttcaacg gctttattac
    25081 gtaaccttaa tatatcaata ggttgtaacc ccttaagtaa catatcatca aaagtctctt
    25141 tgtattcatc aaatattggt tgcaattcat tgtttattag taaacccgga ttattgaatt
    25201 cttttttttc gaggtactct ttagcatctc cgggaatata tattccttct tcaatatatt
    25261 gaagtatatc tttttgattt atttcattat tttgttcagc cgcctctttt cggtaaagct
    25321 ccaaagacgc tgcagtatat tcattgccgt aacgctctaa catttcgggt ttaccataat
    25381 ccgtcattat tacttcagaa tttgtgacac agattctttg ccatatgtcc attccgatat
    25441 catgtgccag cactagatag ggctgaccag tgctgcccca ctccgtaatc cagctttcac
    25501 cgtcaacatc ttccattttc atgcctccgg caagaacaac attaccggga ttatcaggac
    25561 acaaacggta ttccagtaac cacatttgga tatcggtagt aaggcttgct gtgccggtat
    25621 ttattttcgt aagggcagta acctttgcct tggttatggc aaatgttccc ctgccggatg
    25681 ggagattctt gccttcatta ttgtaataat ccacttgctg tgttacaaat tccactgcat
    25741 agttcaagac agcttcagga atatatcctc ttggttttat gctgacagct acacccaagt
    25801 ctttcgtagc actattagca tctgttcctt ctccaatccg ctgatgagat atgaacagat
    25861 aatcgtcacc ttgttttttc agggtaagtg tcacaaattc gttaaacaag cgaacaatgt
    25921 ctccctgagt ttcaccatgg gtgcagtaaa aaataccggc cgcaaaatcg ctggtaaagt
    25981 tatagtaagc atcgtattcc tttatatata ttaaatcttt tgtacccacg ccacttaggt
    26041 catccaacgt gatacccata tttttttgta gtgcttcatt cacggttttg gcagaaaact
    26101 tgtggatagg tacaggcatg cgctttagtg ctatatcatc accaaaaggc cagttttttg
    26161 cctccttaag tgctttaaat tcagtttcat cagtaacatc ttcatccggc gggaaataac
    26221 gcaagaaatt gggaagatca atatcctcag gtttgtcata ataggatgta aaaaagtgac
    26281 tgagtgggtt agggctgtca ttaccctgtt catcaaaaat aagcggttca aaaattttgt
    26341 tatatctctg aacttccttc tctgtaaggg gaacaatttt gtttttatta tccttagccc
    26401 cggtaaaagt gcagcccaca gcaactgctg caattagtag cacggcaatt aatgtatagg
    26461 tagccatttt gttttttttt gctactaatt ctatacgctc tttaatactc cttttgctac
    26521 cagtcatagt ggtcgtcgca gatagcaatg atcctacatt gcgttttgta catgtaagac
    26581 cgatgagtgt acggccgtat tccacgcggc tatcttctcc catacgttga agcgtagtct
    26641 catcgcagga cagttccgca tcctgtacag agtatgcagc agctatccat accagcggat
    26701 tataccagtg aagagcgagg cacatgcacc taagcaacga ccatatatga tctttatgcc
    26761 gccaatgagt aatttcatgc tccaagacat aacgcagtac cgtttcatta tctaatacct
    26821 gcgtagtaac atagattaca gggcaaaata atccaaacat acagggtgtc tctacctcat
    26881 cggttccata gataggcagc ggatagccaa ctatatctgt tttatatcgt atttctttta
    26941 acttatatgc aaaatatata ttagatataa tcaaagtcaa ccctatggcg ccaataccga
    27001 tgagccaaat aatctttgca atttgttccc agttaaatag cccgttatat gagaccgaag
    27061 ccgtgttttc attatatgta gcagttgatt ttgagataag agaattatta acatgtttca
    27121 cattagtagg taagtttacc ataacagtag gtatattctt taacgtttcg ttctgtattc
    27181 ccgcattctg gtcgtaagtt tgaaatatat tcatcacact gacggaactt tccacgaaac
    27241 tcaccggtat aaccaggcgg agaaggacaa ttacccatag agcatactgc aatcttaaat
    27301 caattttacc cttgagcaca tgccgtaatg ctattattat tagaataagc acagacgaac
    27361 ttactatcca ttctctcatt tgttcgcctc ctccgcctga cgtaaaatcg tatacaactc
    27421 ctcaatttcc acacgactaa gctcctgctt cttagcaatt gtactcatca tcatgctaac
    27481 actaccttta taaacgcggc ttagaaaact attcgtctca tgcataactg catcctcacg
    27541 ctttactaga ggggaatagg atttgattcc atcaatttct tcgcatttta taataccctt
    27601 ttccgtcatc cgccgaagca gtgtcaacgt cgtactacgt gcccatccaa tgtgttcact
    27661 aaggtactca gtggcctcac gacccgtgca cggaccagat tcccataagc attccataag
    27721 gttccattcc gtcggggtga gatttatatt attttcggtc attgtatcac gccccctatt
    27781 gtttacattg taaccatatc atataggatt ttgtttacat tgtcaacaga aacctgatta
    27841 gggttttaaa gatgcaatat aacagctata gtgacaaggt gaatatcaaa gtacaattac
    27901 taaaatttcc agggcctccc aggtttcact ttaccccaaa tgcttgttta gtatataata
    27961 tatgtatagg ccatagctga taattgctag caagagtttg tatagggagg gagggctacg
    28021 taatgctgaa ggtaattttg gtggaagatg aaaagatact cctggaggat ttaagagata
    28081 acttctactg ggcttctgcc ggaattgaga ttacagaaac agcacgcaac ggccgcgaag
    28141 cccttgagaa aataaaagca aataaatccc aggttgtcgt cactgatatt aaaatgccgg
    28201 taaaggctgg gatatgggat atggtcgatg gcaagcatgt accgaagcag gaaattacag
    28261 atttgtatgc taaggactat gctaccagct taaaacaggc aggcacaaga aagtatgcat
    28321 ttatttttaa accggattaa tcgaggacgg aacgccatac gacatgcttt atagatataa
    28381 acgtgacgat atggcacaat ttgcaataaa gtatcttctt gacagctgct gaaactggcg
    28441 aaaggtaaat aacaagaagc aagtaggtcg gggaagaggg caggcttata tgcagacaag
    28501 atcggttctt caaatgcatc actgtttgtt gatattgtag cattgggtgg aagttataac
    28561 ccaacaatta tattggtttc aatagatata atgcctggca atttccccga tagtaatcta
    28621 tatgccaatg ttgttgaaat gcaaagatag ttataatgtt aaagctccag aagaaaaata
    28681 atcaagagga aaatacttat ataaaaaagc tccaattgta aagtaaaatg gctgaaaacg
    28741 aaaatgctgg cttaactaaa gcgaaaatga tactggtgga ctacttatat attctggcta
    28801 tctcattata taattttgta ttatcttgta aaaaatcagt gtgattaaaa ccttgatgat
    28861 ataatatgct tgtttcccta accatactta atttttcaat aggtaggaca gtaatttttc
    28921 ccttacttag ttcatcaagg catgcactgc gtgcgaaaat agaaacacca taatatcgtc
    28981 gaataagatc ttttatggtg gcaatattat caacttccaa tataacatta aattctgcaa
    29041 gtgacatatt gagactttcg aggtgtgcaa taaataaatt cctggtgcca gaattaggta
    29101 agcgtaaaat cattttttct ttttttatat cattaattgt taccatactt ttctttgcaa
    29161 gcctatgatt attgggaacg acaagaacta aataatcagt gccaagcaat aaggaattta
    29221 tacttgcatc gtttactttg ccctctacaa ttgctagatc taattcataa ttcattacat
    29281 attataaatc ggctttaaat ataaatttac ttgacaagaa aaaacaaagt tgatataatg
    29341 aacgatgttc agtttggagg tatagcatga ataaattaat cacttcccaa gaagcaatac
    29401 ttcgtgccgg gaaagaaatt gttcttgaaa aaggtttgca aggactgaat ataagggatg
    29461 ttgcgagaag gtgtggcgta tcggttggat ctatatataa ctattttcca acgaaaagtg
    29521 acttaatggt tgctaccatt gagtctgttt ggaaagaaat tatgcatggt tataaaattt
    29581 gtgaagaaca gcatagtttc gttgatagca ttcaatcttt gtttcttaat attcaaaaag
    29641 gttctgaaaa gtacccttct ttctttagtg cacactcgat gagtgtggct actcttgata
    29701 aagaaaaggg gcgtgaaagt atggacaaat acttttcgca catgaaaaag ggccttttag
    29761 caaccctcaa tgccgacagc ggggtcatgg gtgatgcttt ttctccagct tttaataaag
    29821 aggattttat tgattttatt tttagcaatt taatgacact actaatggaa caagctagtt
    29881 cctgcgactt tttattagaa gtgattcggc ggattatata caagtaatta ttataatagt
    29941 ataagtaata ccatccgaac aggatggtat tattttaaac ttaaatgaac aatgttcatc
    30001 attaatattt tggaggttaa ttatgcaagc gattatggaa actttatttg atgtagtata
    30061 tcttactaca gtagtaacat tgggtgtttt gatgataaaa aacaacaatg gcaacaagca
    30121 gtgcacttta tttggtatta tggcggtggt gttgggttta ggagattctt ttcaccttgt
    30181 ccctcgcgcc attgcattat gcacaaccgg actggaaaac tacaccgtat cacttggtat
    30241 cggtaaattc atcacatcca ttaccatgac tgttttttat atcttacttt actacgtctg
    30301 gagaaaacgc tatgatatta gcggaaaaca agatgttacc atagctgttt atctgcttgc
    30361 ggcaattcgg atcattctgt gtttcttccc acaaaatgcc tggacaagtg cgaatccatc
    30421 tttgtcatgg ggaatttaca gaaacattcc ttttgctctc atgggactgc ttattattgt
    30481 tctgttttat aaaagtacaa aagagcataa cgacaaatct ttccgtttta tgtggcttac
    30541 cattgtattg agctttgctt tttacgtccc tgttgtgttg tgggcagatg taagtccact
    30601 ggtcggtatg cttatgattc caaaaacctg tgcctatgtt tggacagtgt ggattggata
    30661 cagtgatatg agaaacaatt ggaaaggggt ggacgcataa tgcaggaaca ggataaacgc
    30721 atccaaatta tgcaggatgc tccaccgctc aaagccattt ttaccatgtc aatccccgtt
    30781 attctcggta tgatggtcaa tgtgatctat aacctggtgg atacctggtt tatcggcatg
    30841 atgggagatg aactgcaact ggccgccagc agctattgca ctccgatttt tgtcatcgtc
    30901 atggcggttg ccagcttgat tggaacaggc ggtgcttcct atctgtcccg cagtctgggg
    30961 gccggccgca aggataaagc cgaaaaaaca cttgctaccg ggctcttgct tgtctgtctg
    31021 ttcggtctgt tggtggcaat cgccgggctt gtgttccgca gccagattcc ccatattctc
    31081 ggagcaagtg agctcagttt taagtttacg caacagtatg ggaccatcct tatgctcggt
    31141 ggcatcttta tcatcggaaa ttttgccctt ggtcagttga tccgggcgga aggctctact
    31201 aaactatcca tgataggcat gatggtgggc gccgttgcaa acatcgtctt ggatccgctc
    31261 tttatcttta cttttggctg gggcatctcc ggggctgctg tggccaccgt catcggcaat
    31321 ggactttcgg tattgtttta tctggtttgc tatctgcgtc gaaagaccct tctgcgtttg
    31381 accttccgtg gatttacgat ggaaccagag atattaaaag agatttttgt gattggcata
    31441 cctgccacaa tcgggcaaat gctgatcagc attgcccaga tgacctgcaa taacctcgcc
    31501 agtgcgtatg gagatgtcac ggtggcctca ctggggatcg ccctcaagat catgaccatt
    31561 ggcacctatg tgtttatggg gttcagtgcc ggctgccagc ccctgatggg ctacaactac
    31621 ggctccggga attatgagcg catgacggaa tttatcaaaa caggaatact atccaccagc
    31681 ctggttgggc tgtttctaac actggtgttg ggagcttcgt ccccctttct tgttgccgtg
    31741 ttcacaccat tggaagaagt ccgcagcagc ggttccgcca tcctgcgcac tctgattcta
    31801 tcccttccct ttatgggtgg cataacgctt tgttcgacta cttttcaggc tatgggtaag
    31861 cctctaaaag ctttcatttt aactatatcg cggcaaggct tattgtatat tcccttattg
    31921 ttccttttag accacctgtt tggcctgagt ggaatggtat actcacaacc aataaccgat
    31981 ttgattatgc ttcttgtcgc cagcgccttt atattaaagg tattacattc actcaaaaca
    32041 ggtctgcgag agacggacaa tttgaaggat atgaagggag aaattgatta tgaaaaaata
    32101 tattaatatt tcgtttattt acgcaatgac cgcaatcgca tgcggcgtat tctatcggga
    32161 gttcacaaag tttcttggct tttctggtaa aacaacttta gcgttcacac atctacattt
    32221 ttttgttttg ggtacaatca tgtttttgct gattgccatt ttcagcagta tcaccaatct
    32281 atcacagcaa aaacaattta gccgttttat gctactttat aatatagaaa caagcaaagg
    32341 ggagaaagaa aatgggagtt aaaaaacatt ctgatgaatt tagaagacag gtttttctat
    32401 atacagtcaa ttattgatgt gtatgacaga gaaattattg attatcatat agggttacgt
    32461 tgtgaaggtg aggatgctgc agcaacactt gtgagggcgc ttatgagacg aaatctattt
    32521 gaaagtgatg taaagcctgt aatacgttca gataatggtc cacaatttac aagcaataag
    32581 tttgaagata agtgtttaga ggtaaatgta gaacatgaaa gaattccgtg caaaacgccc
    32641 aacaaaaatg ctcatatcga atcatttcat cgtatatttg aagatgaatg cctgggacat
    32701 cgggagtttg agagttatga agatacatat aaaaaggtct tacatctttt tataagttcc
    32761 ctttataacc ctcgtgaata aagggtttca aaaaaaactc cccttatcat cgtataaggt
    32821 tttatattgt gttataacgc ctcaggtaac aaaaaggtaa caaattttaa tatatatttt
    32881 cctccaaatc cagacaaaac aataagctac tttaatttat ttcttcactg tttaatatta
    32941 tagaatatgg aaggttaaag attgatttgt tcgcatcctt gccggtaaat aacagatatg
    33001 tgaactacac cgctattgaa atagcgggct tcgggttccc tatgaaatta tg
//
LOCUS       DAOSDD010000019        32254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_682_length_32254_cov_5.357589, whole genome shotgun sequence.
ACCESSION   DAOSDD010000019 DAOSDD010000000
VERSION     DAOSDD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 32254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32254
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_682_length_32254_cov_5.357589"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PK733_03145"
     CDS             <1..706
                     /locus_tag="PK733_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOJ09570.1"
                     /translation="TVSGINPIARAFEFYFPIVIFIFFSVLFLGLQRFELDNLRPLFG
                     QGFKPVLRGIMTTFLTYAGFETMMILTAFMKEPWKAVKVVVIGIGISIAFYVSLSVVV
                     VGVLTVEEIKTLAWPTIALVNSIEYPGGFVENFQVFFLIVWIFAIYTTFVSSHYIACL
                     GLGQISNKAFNSFVYALIPIIYIVALAPQNLEEVFQLGDYIGYFGVFMGGIVPVILLF
                     IAIIRREGCAEKQGEK"
     gene            681..2051
                     /locus_tag="PK733_03150"
     CDS             681..2051
                     /locus_tag="PK733_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ09571.1"
                     /translation="MRKNKVRSKVTILVGLLITLAVFLSGCWDRREIEELSMVAGLAI
                     DCEENTSTPYKEGSISNKEEQMEFDKEKQLQIIVTQQYIVPKAVSGGEKGGTSQKSPY
                     ANVSVDGNSIYEIMQKFENINSRTPNYEHLRVIIISDKAARYFNLYKLLNAILRHPET
                     PRNIDMLVSERKACSILDIAPRSEDIPAFKLMMLVRKTKSKSGTPSVSTLGDMSKAMA
                     AGKSFVIQRVGFRQDIGSEYDTVKISDSNYNNLANINKTSGMNFIYDSGTKGLASGMG
                     DIIEAVGAAVIKGQTYNMTGWLNRSETDALNWITGKVEEGSVEFKESKSSETIVYDVD
                     RVKTKIKPEILEGKISFTVDMKMEGTLVEDWLMTADAFKEEFIKDMEMNAEKEVRRIL
                     EKTLEKLQKELKTDVAGFGKQLSIKYPRYWNDIKDNWEEIFSDVPINLDLKVYIRDFG
                     RQGSKK"
     gene            2253..3632
                     /locus_tag="PK733_03155"
     CDS             2253..3632
                     /locus_tag="PK733_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ09572.1"
                     /translation="MIKFNKRTNTLEQTLYKYSLQDVPDPNLYRDVFTYHEIPKCTFN
                     HRRVPMFPADKIWITDTTFRDGQQSREPYTVEQIVTLYDMLHKLGGPNGIIRQTEFFL
                     YSDRDKLAVYKCMERGYDFPEVTGWIRATKSDFQLAKDMGVKECGILVSCSDYHIFKK
                     LNMTRKQALEHYLGIIKGAIDVGIKPRCHFEDITRADFYGFVVPFAIELRNLIDEYKI
                     PIKIRACDTLGYGVPYPGAALPRSVPGIIYGFTHYAGFPSELIEWHGHNDFYKAVVNS
                     ASAWLYGASGVNCSLLGIGERTGNTPLEAMIIEYAQLRGTTDGMDTTVITEIADYYEK
                     EIGYSIPSRTPFVGKHFNVTQAGIHADGISKDEEIYNIFDTTLLLKRPAGVAINQSSG
                     LAGIAHWINSYLGLSGNIKIDKKDPRIAQVKEWIDMQYANGRVTAIGDDELLAVIKEL
                     VPETLDLVL"
     gene            3834..5765
                     /locus_tag="PK733_03160"
     CDS             3834..5765
                     /locus_tag="PK733_03160"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOJ09573.1"
                     /translation="MNLNLVQKIIKEHLISGEMVPGKEIAIRIDQTLTQDSTGTMAYL
                     QFEAMGVPRVKTKKSVAYIDHNMLQTGFENADDHKYIQSVAAKHGIYFSKPGNGICHQ
                     VHLERFGVPGMTLLGSDSHTPTAGGLGMLAIGAGGLDVAVAMGGGPYYITMPEVYRIN
                     FKGSLKPWISAKDIILEVLRILTVKGGVGKIIEYAGEGVKTLTVPERATITNMGAELG
                     ATTSIFPSDEVTREFLKAQGREQDWIELKPDSDAQYDKELEIDLDKLEPLAAKPHSPD
                     NVDKVKNIGGIKADQVAIGSCTNSSYVDMMKVASILKGRTISPEVSLVIAPGSKQVLT
                     MLAQNGALAHMVAAGARILESSCGPCIGMGQSPASDAVSLRTFNRNFEGRSGTVSAKV
                     YLVSPETAAVSAITGVLTDPREFGEEPKIDMPERFLINDNMILASVPASDSDDIEVVR
                     GPNIKPFPINKELAGTVSGKVLIKVGDNITTDHIMPSNAKLLPFRSNIPHLSQYCLTP
                     CDPEFPKRARENGGGFIIGGSNYGQGSSREHAALVPLYLGVKGVIAKSFARIHMANLI
                     NSGILPMTFVEEKDYENIDMDDDLVLENAREQVKNGPVLLITNKTKGKEIKVRVALSE
                     RQIEIILAGGLLNYTKNRN"
     gene            5843..6535
                     /locus_tag="PK733_03165"
     CDS             5843..6535
                     /locus_tag="PK733_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ09574.1"
                     /translation="MLRFVNNGEDRTATSLGAKIFKQLQNDILNGKYEPGDILTEMKL
                     SEELGVSRTPIREALKQLELEGLVKSTPGRGVVVQGITDKDIEDIYTIRMMIEGLAAR
                     WAAEKITPGELEELKEILEFEEFYTLKGGGTGNLLKLDSRFHDLVFKASKSRPLMHIL
                     STFHHYVQSARSASLETPGRAQKVFEEHRAILQAINARDPDLAEKLTTEHVKNANINL
                     MKKRSEQEHKQE"
     gene            6552..7919
                     /locus_tag="PK733_03170"
     CDS             6552..7919
                     /locus_tag="PK733_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOJ09575.1"
                     /translation="MSDKIADREIKDMIEDLSMLAKRNNYINPELYSRYNVKMGLRNS
                     DGTGVLVGLTEIGDVHAYIMDENEKVPVDGRLIYRGIDIYDLVAGFQNEKRFGFEECC
                     YLLLFGQLPTQRQLEEFKWLLAEHRNLPNGFTRDLILKAPSKDIMNKLARTVLSAYSY
                     DENPDDTSISNVVRQSIELIASLPTLAAYGYQALAHYYEEKSLYIHNPRHDLSTAENI
                     LHMIRPDSKYTQSEAEILDLALVLHAEHGGGNNSTFAIHVVTSTGTDTYSAIAAGLGS
                     LKGPKHGGANIKVMQMMENIKDNIKDWEDDDELTAYLEKIMNKDAFDKSGLIYGMGHA
                     VYTLSDPRAVLLKKKAEGLAAEQGFMKEFKLFERVEKLSPKVFQKAKNTDKIISANVD
                     FYSGFVYKMLGIPMELYTPIFAIARVAGWCAHRLEEIIDGGKIIRPAYKNVGEKQDYI
                     PLSER"
     gene            8245..9483
                     /locus_tag="PK733_03175"
     CDS             8245..9483
                     /locus_tag="PK733_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ09576.1"
                     /translation="MELNMNKKVNTICYMLMLFLGIYLAMYQNSINYITEGVLNKSTI
                     MGVLVALHFIGIIIAPVTFGEAGDRIGKKKVLTIAFATILFGISIVFVSKSIILVAVG
                     ILFIGCGFGAIESLGSSILADENPYDTNRVINISQVFFSAGAVMGPLISLKIVEISGS
                     WKISYLIAFIFFASMLVATSKTDFGGVTGTGTDSITSTGAVSSAGIGTDAAACKDTGS
                     VAVTTKKTGLYSSRLLKDRYFQILCLSIFLYVGIEGASSFWITTYFRDVLGAEKLGSY
                     ALSGFWGSMIVGRYIGSKLESKSRIVMNISLVISIVSITSGLLIKSTISGLVSFTLLG
                     FGFAVIWPVLMTMAAKRYPEKTGTAMGIMMTISAVGGMIIPFIMGILGDALSISAGLS
                     VVPASALLIILLQFKAVTVE"
     gene            complement(9621..11030)
                     /locus_tag="PK733_03180"
     CDS             complement(9621..11030)
                     /locus_tag="PK733_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ09577.1"
                     /translation="MLIHVVRPGESLWAIANQYRVSIAAIVDINELPNPNQLAVGQAL
                     VIPTEDVFYTVKPGESLWRIAQIYGTTVEAIVQSNQITNPNNIYPGMVLYIPARRHRV
                     QPGETLWIIAQRYGVTLQSLMKVNNIENPNLIYPGTVLVIPRKQRPVIDVNGYIYFLG
                     EEAVPIVRKHGKDLTYLSPFAYLIREDGSLQSINDIPAVQAAKAENAIPMMAITNFTA
                     TTVGENLAHIVLNNPQIIESLLANILNTMKAKGYQGLNVDFENVLPEDREAYNHFLEK
                     TVARLHPEGYFVSTAVAPKTGPEQAGLLYVAHDYPAHGRIVDFVILMTYEWGYRKGPP
                     QPISPLNQIRRVLDYAVTVIPREKIFFGFQIYARDWLLPFVPGQEAETFSCQEAVLRA
                     IRYGAAIQYNSIAQSPFYRYIDAQGRAHEVWFEDARSAQAKFDTVKAYNLKGISYWAL
                     GYPFPQNWALLEDNFTIRK"
     gene            complement(11100..12173)
                     /locus_tag="PK733_03185"
     CDS             complement(11100..12173)
                     /locus_tag="PK733_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ09578.1"
                     /translation="MAKLTIKEIAKMAGVSPTAVSFVINNREGVSEETRKIVREVLER
                     TNFQPSLNSRRLLLNKSFNISIVIRKTSSPFDDLFYLEIAKGLLEKSKEYGYNIVFTD
                     ISIENGRAILPDIIKNEDTDGIVFFQDTEDIILNEINKLDIPYVIADAHNSENSASSE
                     KPDILNNSTSSGASFSDITSVNADYELAAYTAVRYLTSNGHKDIAYISSSFVPNFYLH
                     TFEGYKRALEQAQVSVQPSWIQVNAINEDSAYNCMKAILKSKFKPTAVFCTTDMFAIG
                     AIKCAKDSGYKVPEDISFISIDDIFLANYIEPKLTTIRIDKFKMGTLAMELIIDKIEG
                     KSTESITVDSDNLVIRESVKKLA"
     gene            12601..12780
                     /locus_tag="PK733_03190"
     CDS             12601..12780
                     /locus_tag="PK733_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09579.1"
                     /translation="MSPELHIRRLAAVKLATAVNKIEGVPVSTQAKTLSAKWARGEIS
                     GAEMKATLIAKHKKG"
     gene            12793..12996
                     /locus_tag="PK733_03195"
     CDS             12793..12996
                     /locus_tag="PK733_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09580.1"
                     /translation="MNDPYLYEGSSVLRNLLNIHDEKELDIAEAELSQANMMLLYEQG
                     FDDFSTYKPVAHEYRADDGEDKT"
     gene            complement(13117..14175)
                     /locus_tag="PK733_03200"
     CDS             complement(13117..14175)
                     /locus_tag="PK733_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09581.1"
                     /translation="MLTARENYIRNARFQYPEWIPSSVHISNASWDQLRYDIEEVALR
                     HPDFFPYVKKGWRDYKNFNFGNAYTKGQPFKDSWGCIWESPVNGLEGVVRNAPLSSWE
                     NFQSYVVPDPDIHADRSIRNWDRERKNIELNKSNNNLTAGGLAHGFLFLRLQYLRGFE
                     NAMFDMYTDEPLLVKLIDEIDQHNIKIVERYLDMGVDIMEIPEDLGMQNSLVISPDMF
                     RKWILPSYKKLFRPCREKNTLVAFHSDGYIMEIANDLIEAGVDIINPQDLCNGIDNIA
                     REIKGKACIRLDIDRQKIIPFGTRKEIHELIEEEVRKLGSPQGGLEFIAGIYPPTPPE
                     NVDALCEALRKFRRYYWE"
     gene            14453..15361
                     /locus_tag="PK733_03205"
     CDS             14453..15361
                     /locus_tag="PK733_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09582.1"
                     /translation="MSGKSKSSKDIFLRERLMINFSHTGINNLITLGRINYLNAHKPL
                     DKHTHKDSMEICYLARGRQTYSVGDKPYTLKGGDIFITFPDEEHSTGINVEERSILYY
                     LIFDIKNNLDNFLGYKGEEGRELALALNNMSRVNRHFAGNSEIRSILDEVFLTFYSAK
                     KFKKIVMQGLISKLFIRIIELENNRKNSFPEDISKTINYINDNIQHELRLDKLASDAN
                     LSLSRFKQKFKEAAGIPPGEYIKRAKVDFSKHLLKDSDSTITEISYQLGFSSSQYYAM
                     VFKKFKGITPKDFRIRVRKDSNYLGL"
     gene            15643..16797
                     /locus_tag="PK733_03210"
     CDS             15643..16797
                     /locus_tag="PK733_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09583.1"
                     /translation="MRRLKAGIVGTGYIGISHIETIRRIGFAELVAVADVNYDMASRR
                     AKEYYIPKCYGTLDELLADKEIDVIHNCTPNNLHLEVNEKIIKAGKHIFSEKPLARNS
                     EESLKLLLLLNENKNIIHGMNYNYRMNPLVQEMKSKVKNGEIGQPKLVHGSYLQDWLL
                     FDTDYNWRIEPEICGPSRCIADIGTHWMDAVQTIIGAKIVELTADLVTTIPIRKKPKG
                     QVETFSITGGTEYEEVEVKTEDYGSVIFKMDNGVRGVFCASEVSAGRKCYLNFEIDGT
                     RSSMYWNQEMADQMWMGFREKDNSLVMRNPNLMTPEAKQYTYLAAGHPEGWNDAMRNN
                     VYSFYKYIADGKTMGKDPCDFATFDEGHYLIKLTEAILESNKTRQWVKVE"
     gene            16936..17967
                     /locus_tag="PK733_03215"
     CDS             16936..17967
                     /locus_tag="PK733_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ09584.1"
                     /translation="MKSQFKFSVGPWNVHEGADAFGPEVRKTIEFAKKVEKFKEIGFD
                     AVQFHDDDAVPDMNNLTDGEIKAKAGEVKKILDNNGMAAEFVAPRLWMDPRTADGGFT
                     SNSKEDREFAMWRAFRSIDIANELGCNKIVLWLAREGTLCYESKNPVEGVKRLIEAIN
                     KMLAYDKNIKILIEPKPNEPIDRSFCPTIGHVMGVSAATNDPSRVGGLLESAHAILAG
                     LDPANEIAFGLAFGKLWGVHLNDQNSIRYDQDKAFGVENLRQAFNQIKVLIENNYGKN
                     GEYIGLDVKAMRTQRDEVCYRHLENSLKIAKMLEEKARKFDYSFQKKCVEERDYEALE
                     MYVMELLMN"
     gene            18167..18391
                     /locus_tag="PK733_03220"
     CDS             18167..18391
                     /locus_tag="PK733_03220"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ09585.1"
                     /translation="MYTKLQLDMIINEITNSVRDILSSKFYKIILYGSYARGDYDNES
                     DIDIMILADLRNDEISALRKKLTKLRVKSV"
     gene            18457..19314
                     /locus_tag="PK733_03225"
     CDS             18457..19314
                     /locus_tag="PK733_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09586.1"
                     /translation="MTILCQKYTTNRDRPFIISSGRGTYFPYHWHDEIELLYVLEGSM
                     TFVIGPKAYNINKRDIVIVKSGEVHRFEAKVACSHLFVEFGHYLDTDLFKLPILIYPG
                     MLSVLRAQNGSREDLNQLHSKVESLMLDMLTEYNQKTDGWEFSVKASLYRIIVLLTRA
                     FYDKIEIEVEHKNITRGYTEILQEVFEYIEGHYNENITIEDVSEIANFSRHYFNKFFK
                     KATGKTFASYINDIRLEKAQAFLKSSNQNITNIAYDVGFNSVKTFNRLFKESFGCTPS
                     EYRGSVRAL"
     gene            complement(19353..20525)
                     /locus_tag="PK733_03230"
     CDS             complement(19353..20525)
                     /locus_tag="PK733_03230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ09587.1"
                     /translation="MYRIGDEEVRAVEKVIKSRQLFRFNEGNKEVASFEKELAEKIGT
                     KYSLALTGGTGALISALVGIGAGPGDEIIVPAYTFMATALAVVAVGAIPVIAEVDDSL
                     TINPDDVEKKISKYTKAIIPVHMLGLPCNMDRIKEIAEKHNLQIVEDCCQADGGSYKG
                     RRLGSIGDAGAFSFNYFKIITAGEGGAVTTNDEYIYEKALIYHDGGTAFRPHAGGLKT
                     PIFIGSQLRSNEIAGAILRVQLQRLDDILSDLRKVKKAFMSRLEGEKNIKFIRSNDIK
                     GDCGSNVGFLLQSENAARKFAASEGVNSGVLIDSKKHVYFDWEPIMKKRGSHHPVLDP
                     FKLLQNKDLNMNYSYDMCPNALDILSRTVIVGMSPDWSEADIEKKAEAYRKSAAAL"
     gene            21221..21973
                     /locus_tag="PK733_03235"
     CDS             21221..21973
                     /locus_tag="PK733_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ09588.1"
                     /translation="MNFFDCNVCYGYDVRNNVMYPVNTINELSCHMEQAGVGKAMVSR
                     VEQYIAGTPSTNQILAEDTANHQNMYGIWGLLPSHTHEIPEGEFMLSIMKEKRIFGWR
                     LIPNIGRYLIKDFVLRDWFKLAIERKIPIFINTAHGTSFEATVEILKAFPELTVILTY
                     PQIWPNDRYLRPFVAEYPNVYLDLSYCITDGGIESFVNEYGAGRLLYGSGFPYCYFGA
                     NMLMIKHADISEDDKAAIAGGNMTGIIKGVLL"
     gene            21970..22818
                     /locus_tag="PK733_03240"
     CDS             21970..22818
                     /locus_tag="PK733_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ09589.1"
                     /translation="MINSKSELAQGFWEKGKLPDCPIIDFHAHMGDNGGIFLPKSTPD
                     AMINTMDACNTILTCFCGHEALFVPSIGEKLDIAAVKKYPDKFKAYHAVVSRYLKPED
                     DIARVRENPDVFIGFKFLCDYYGVPLSDSRHDKYWEYANDNSLLVLTHTWGGSNHNGC
                     KEAEKVLSKYPDLVFIAGHSFHGEWDKAVELTKKYPNLYLELTAVLDDRGPLDMFVQE
                     IGSERILFGTDLPWFSTHHGIGAVLSAEMTDEDRRNIFYRNGVKLLKKFSWFEYMWKD
                     RQSCIQ"
     gene            22877..23698
                     /locus_tag="PK733_03245"
     CDS             22877..23698
                     /locus_tag="PK733_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ09590.1"
                     /translation="MGKAKFAYTVWQWGTDNKEQLRQALADIREAGFEYFESVKATIN
                     VFDNDVSGFKALCEEYGVKPVGFYFHFNGDWENDVGDVKRKLPFMAACDIHRMNVQAT
                     GVVGRPATKEELDYNIKAINAIAKLAKEYDIVPTMHNHYNTTVMYESEIDYMLQNLDP
                     DLVAFGPDTAHLVAGQCDPVKVIKKYADRVKFTHLKDITNEGMEAAGMQAGVEVYTNF
                     RELGEGIVDFPSIFKILDDAGYDGFLTAELDRSRFGNKESAIMSMNYLKANYKQD"
     gene            23775..24059
                     /locus_tag="PK733_03250"
     CDS             23775..24059
                     /locus_tag="PK733_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09591.1"
                     /translation="MSESLEVGVGRADITPALAVQLMGYRVIRRESINFYWFIQIIFC
                     ILKNWLKDVVSDEMHIHIFIQGQMYFSSSRDIPSLILYRRYFSIEILTFL"
     gene            24247..25497
                     /locus_tag="PK733_03255"
     CDS             24247..25497
                     /locus_tag="PK733_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ09592.1"
                     /translation="MAGPGSYLFGEEERNELLDVMESGYLFRYGDEENPCFKRKVATF
                     EKEFAEYSGINHCVAVSSGTGALMCCLAAIGIGPGDEVIVPGYTFIASISSIILSHAI
                     PILAEINESLTIDPEDIKKKITGKTKAIMPVHMLGNPCDMDEIIKIAEEYNLYVIEDC
                     CQAAGASYKGRKAGTIGHMGAFSLNIFKTITSGDGGMVITNDYDLYERAFAFHDQGHK
                     PNRMGVEVGKRSIVGMNMRLNELSGAVALAQLRKLDTILALLHEKKKKLKDSIEAYIK
                     DIPDVYFRKLNDEGECATLMTLLFKDKQKADLFAEKIGAKTIVHSGWHVYNNMEQILG
                     KKTATDYGCPYVCEAYGREIEYKAHMLPITDNILERAVNISVGVVDKGLGSPFGINIT
                     STDDEIAEVACEIITLLKQVTLRT"
     gene            complement(25589..26461)
                     /locus_tag="PK733_03260"
     CDS             complement(25589..26461)
                     /locus_tag="PK733_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09593.1"
                     /translation="MGLELKTFKKDIDILGLYTFYYFEHSKDFYFTGERHNFWEMVYV
                     DSGEISVIADNAGYIIKQGDVIFHKPMEFHTLASNKKDPHNVMVITFSVKGEAMKFFE
                     NKIFTVDSQHKKILSLLLEEAQKTSFSIPNNLYKKLLDIKNSEIGSQQLVISYLEQFL
                     IALMRSNQNNERNGRISRYAKKNAENALVDSIEKYLLDNIYERISLKDICEKFNLSKS
                     YICHLFKEATGKSIIDCYINFKIAEAKKLIRQGNLNFTQISEKLCYTSIHHFTRSFKN
                     ITGISPSSYEKSVK"
     gene            26861..27862
                     /locus_tag="PK733_03265"
     CDS             26861..27862
                     /locus_tag="PK733_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09594.1"
                     /translation="MHNIVLIGCGHMGTAHLEDIYLREDICVYGVVDIMEEKARLTAK
                     KYNAKSFSTDYIRYMEDENVDIIICATYPSTHLQILKDCVKYNKHLLCEKPITDNLED
                     GKEFVSIVKNTSIKVLIGYILRYNETYKKVAEMIEEGAIGGPIVLRMTQNNHTVEWEK
                     YLALIKETSPIIDCGVHYIDVCRWFTGAEVIDVGGISLTTEKDVPKGKYNYGMITMKL
                     SDGSVAYYEAGWGKTVAADNTKEFIGPKGRIKIVQSHNRFFNKEEGDLIEYYNVENKE
                     YKIINVNCKRRPTGAELDNLINMIENNAPSNPSIEDVYKSFEIAIKADDIIRPDICF"
     gene            28042..29193
                     /locus_tag="PK733_03270"
     CDS             28042..29193
                     /locus_tag="PK733_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09595.1"
                     /translation="MKVGIIGFGGRIRGMFKALERQEIGDITLSAITDIRNDEIKELL
                     TKEGKDPEKVSFYEDPDKMMDNEELDGIMIGTRCSLHTKMAVKVLEKNIPLFLEKPVA
                     THMDDFFKLKEAGEKTKAQAVISFPLRTSIIATTVKEIIDSGKIGTVEHVQAINNVPY
                     GRVYFHHWYRDENETQGLFLQKATHDFDYINYLLGSKPVEICAMTSKQLFKGDKPAGL
                     YCKDCDEYYTCDESPYVIKKYYKDNPIGEMCCYAKDTGNEDSGSALVRYNTGMHVSYS
                     QNFFARQKAGARGARLFGFKGTLEFDWYTGEIKVFMHHTPRVDTYKFDMSGGSHFGGD
                     DVLAENFIKVMQGIENSIAPLEAGLLSALMCIKGRQSAITGEFKKIDEM"
     gene            29570..30592
                     /locus_tag="PK733_03275"
     CDS             29570..30592
                     /locus_tag="PK733_03275"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09596.1"
                     /translation="MNENRQKRISEIVHNVLWYNENPGKNYLQRVIAIVEKALGVNIT
                     YININRNTASDSRLDILRNNAVHSSSYCLFIKEKIKGQKYCYICKMKGIKKCMTLMEE
                     FDGMCIMGVHDIVKPVIKDDELFGILYLSGIRYTPEECKARELINKHCKLLGRYEEEM
                     LKEYLRLTPVNIEGLGVMEKCLEELRQIILDILNTMKPLNVGGKEISGSSGASASDKK
                     WIYNTILPYIEENYKNNVSLEELAETFFINSQYLCRLFKSKTGMNFKQYITQLRIEEA
                     KAKLTVPINSITDIALSTGFNDPNYFSRVFKDNVGMTPSEYRKSACPCSQFSASCSQK
                     IKGFID"
     gene            30750..32018
                     /locus_tag="PK733_03280"
     CDS             30750..32018
                     /locus_tag="PK733_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ09597.1"
                     /translation="MENHSKYDVIVAGGGLAGTLSAIACAREGLKVLLIERYGFLGGM
                     AVSGLVNPFMPYFSKSKIDGSEIPVNNAGLFQSLLDRLSEKDSLKNRSIFNEQVLKVI
                     LDEMILENNVDVLFHSFVFETRKEGNLIASLKVVGKSGVREYEADYFIDATGDADLSY
                     LSGCETKQGRDEDGLCQPMTTCFRIKKSASAENEPKMSRADINKIYNQAQKEGKIKNP
                     RENVLIFDHTCPDVVHFNSTRIVGKSAVDAEQLTEAELEGRRQVYELYCFMRDNIPEY
                     RDSVLLEIAPQVGIRESRRIVGKYVLTGEDVLNCVQFEDAIARACYDIDVHNPAGTGT
                     VIKRIKGDYYTIPLRSLIPNGADNLAVAGRPISCDHEAHSSLRVLPIASSIGEAAGCA
                     VAAAAKSKLTLDRVNYEDVQNMLSKYGAIY"
BASE COUNT        10713 a         5196 c         6617 g         9728 t
ORIGIN      
        1 gactgttagt ggaataaatc caattgcgag agcatttgag ttttattttc ctatcgttat
       61 atttattttt tttagtgtac tttttttagg gcttcagcgc tttgaacttg ataatttacg
      121 gcctttattt ggacagggat ttaagcctgt attaagaggt ataatgacga cttttttgac
      181 atatgcggga tttgaaacta tgatgatact gactgctttt atgaaggaac cctggaaagc
      241 tgtgaaagtt gttgtaattg gtataggcat atccattgct ttttatgtgt ccttatcagt
      301 ggttgtagtt ggtgttttga ctgtagaaga aataaagacg cttgcatggc ctacaatagc
      361 cttggttaat tcaatagaat atccgggagg gtttgttgag aactttcaag tgttttttct
      421 tattgtttgg atatttgcaa tatatactac atttgtttct tctcattata tagcctgtct
      481 gggtctggga cagatttcta ataaagcatt caacagcttt gtgtatgctt tgatcccaat
      541 aatatacatt gttgcccttg ctcctcaaaa tttggaggaa gtttttcaac tgggagatta
      601 catcgggtat tttggggtat ttatgggagg aatagtacct gttatattac tttttattgc
      661 aattatacgg agggaagggt gtgcggaaaa acaaggtgag aagtaaggtt actatactag
      721 tgggtctctt aataactctt gctgtatttt tgtcgggatg ctgggataga agggagattg
      781 aagaattatc tatggtagca ggtttagcca ttgattgtga ggaaaataca agtactccct
      841 ataaggaagg cagtatttct aataaagaag aacagatgga gtttgataaa gaaaagcagc
      901 ttcagattat agtaactcag cagtatattg tacctaaagc tgtaagtggc ggtgaaaaag
      961 gaggtacttc gcaaaaatcc ccttatgcta atgtatcagt tgatggtaat agcatttatg
     1021 aaattatgca aaaatttgaa aatataaaca gccgtacacc aaactatgag cacttaagag
     1081 ttattataat cagtgataaa gcagctcgtt attttaatct atataagctt ctcaacgcaa
     1141 tactgcgcca tcctgaaact cctagaaaca tagatatgct tgtttctgaa agaaaagcct
     1201 gttcaattct tgatatagct ccaaggtctg aagatatacc tgcatttaaa ctgatgatgc
     1261 ttgtacgtaa aacaaaaagc aagtcaggaa ctccttctgt atctacatta ggtgatatgt
     1321 ctaaagcaat ggctgcggga aaaagctttg ttattcagcg ggtaggattt cgccaggata
     1381 ttggcagcga atatgataca gtaaaaatat ctgatagtaa ttataacaat ttggcaaata
     1441 taaataaaac aagtggcatg aattttatat atgattccgg tactaaaggg ttggcaagtg
     1501 gtatggggga cataatagaa gctgttggag ctgctgttat aaagggacaa acatataata
     1561 tgaccggatg gctcaacaga agtgaaactg atgcattaaa ttggataacg ggaaaagttg
     1621 aggaagggtc ggtagagttt aaagaaagta agagcagtga gactatagtt tatgatgtag
     1681 acagggttaa aactaaaatt aaaccagaga tattggaagg taaaatttcg tttactgtag
     1741 atatgaaaat ggaagggact ttggttgaag attggctgat gaccgcagat gctttcaaag
     1801 aagaattcat aaaagatatg gagatgaatg cagaaaaaga ggtaaggcgt attttggaga
     1861 agacactaga aaagttgcaa aaagaactaa aaactgatgt agcaggtttt ggaaaacaat
     1921 taagtataaa gtaccctagg tattggaatg acataaagga caactgggaa gaaatattta
     1981 gtgatgttcc tataaatctg gatcttaagg tatatatcag ggatttcgga agacaaggct
     2041 caaagaaatg aatataaaag ggatactcct caacgtaaaa ggaatatctc ctgctcgaga
     2101 gaggagtgtc cccttacctt acaaaaaaac tcttgacaac aattacatac ctgtatacaa
     2161 ttatacacat atacattttt ttgagtgggg aatttgcttc taaatgctat aggaagctag
     2221 tttaagaagc aatgcgtttg gggggaatat agatgataaa gtttaacaaa aggacaaata
     2281 cactggagca aaccttgtat aaatattcct tgcaggatgt accggatcct aatttataca
     2341 gggatgtatt tacttatcac gaaataccaa aatgtacatt taatcataga agagtgccaa
     2401 tgtttcctgc cgataaaatc tggataacag atactacttt cagggacggt caacaatcca
     2461 gggaaccgta tactgttgaa cagatagtaa cactttatga tatgctgcat aaactggggg
     2521 ggcctaatgg aataatacgc cagaccgagt ttttccttta tagcgacagg gataagttgg
     2581 cagtatataa atgtatggaa agaggctatg attttcctga agtaacaggc tggatacggg
     2641 caacaaaaag cgattttcag cttgcaaagg atatgggcgt aaaggaatgc ggaattcttg
     2701 taagctgctc tgactatcac atttttaaaa agctcaatat gacaagaaag caggctcttg
     2761 aacattatct cggcattata aagggtgcaa ttgatgtggg gataaaaccc aggtgccact
     2821 ttgaagatat aaccagggct gatttttatg gttttgttgt tccttttgct attgaactta
     2881 gaaaccttat agatgaatat aaaataccaa ttaagatccg tgcctgtgat acgctgggct
     2941 atggagttcc atatccggga gcggcactgc ccagatctgt gccgggaatt atttacggat
     3001 ttacccatta tgccggattt cccagtgaac taatagagtg gcatggccat aatgattttt
     3061 ataaagctgt agttaattct gcatctgcat ggttgtatgg agcttcaggt gttaactgtt
     3121 ctcttctggg aattggagag cgtaccggca acactcctct tgaagccatg ataattgagt
     3181 acgctcagct aagaggtact acagatggta tggatacaac ggttataact gaaattgccg
     3241 actactatga aaaggaaata ggctatagta taccgtccag gacacctttt gtgggtaaac
     3301 attttaatgt aacacaggca ggtattcatg cagatggtat atctaaagat gaagagattt
     3361 ataatatatt tgatacaaca ctgctcctta aaagacctgc aggggttgct atcaatcagt
     3421 cttccggtct tgcaggcata gcccactgga ttaattccta tttaggttta agcggaaata
     3481 taaaaattga taaaaaagac ccacggattg cacaagttaa agaatggatt gatatgcagt
     3541 atgcaaatgg acgtgttact gcaataggcg acgatgagct tctggctgtt attaaggaat
     3601 tagtccccga aacactggat ttagtgttat aataactata tataacgcaa aaatgattta
     3661 acaaggtaca aaatggattt ttgcgtatta attaaggtaa cataggcgca caaaatttta
     3721 taatgtagta tattttgtac attatctata gagcagagga cttatctgct tactgatatg
     3781 ataaatgctg ccaatgagtt tttgcataat ttaatcatcc aggaggttta ataatgaatt
     3841 tgaatttagt acagaaaatt ataaaagagc atcttataag cggagaaatg gttccaggaa
     3901 aggaaattgc cataagaatt gaccagactt taacacagga ctccacaggc accatggctt
     3961 atttacagtt tgaagctatg ggagtgccaa gagttaaaac aaaaaagtcc gttgcttata
     4021 tagatcataa tatgttacag acaggttttg aaaatgctga tgaccataaa tatattcaat
     4081 ctgtagcagc taagcatgga atatactttt caaaacctgg aaatggcata tgccatcagg
     4141 tgcatctgga aagattcggt gtaccgggca tgacactttt agggtctgac agccatacac
     4201 caacagccgg aggacttggg atgcttgcca taggtgcagg cggccttgac gtagccgtag
     4261 caatgggcgg cggcccatat tatattacta tgcctgaggt ttacaggata aattttaaag
     4321 ggtcattaaa accatggata tcagccaaag atattatttt ggaagtacta agaatactta
     4381 ctgttaaagg cggagttggt aaaattattg aatatgcggg ggaaggggtt aaaactttaa
     4441 ctgttcccga aagggctaca ataaccaata tgggtgcaga actaggtgct accacatcca
     4501 tttttccaag tgatgaagtt acaagagagt ttctcaaagc ccaaggaaga gagcaggatt
     4561 ggatagagtt aaaacctgac agtgatgctc aatatgataa agaactggaa atagatctgg
     4621 ataagcttga gcctcttgcg gcgaagcccc atagccctga caatgtagac aaggtaaaaa
     4681 acataggcgg aataaaagca gatcaggtgg caataggcag ttgtaccaat tcatcatatg
     4741 ttgatatgat gaaagtagct tcaattctga aaggcaggac tataagccct gaagtaagcc
     4801 ttgttattgc gcctggttcc aaacaggttt taacaatgct tgctcagaac ggtgctcttg
     4861 cccatatggt tgctgcaggt gcaagaattt tagaatcatc atgtggacca tgtataggaa
     4921 tgggtcaatc ccctgcttct gatgcagtgt ctctacggac atttaataga aattttgaag
     4981 gaagaagcgg cacagtttct gcaaaggtat atcttgtaag tcctgaaaca gctgccgtga
     5041 gtgcaataac tggtgtttta accgatccgc gtgaattcgg ggaagagccg aaaatagata
     5101 tgccggaaag gttcttaata aatgataata tgatattagc gtcggtgccg gcctcggata
     5161 gtgatgatat tgaagtagta aggggaccta acatcaagcc attccctata aacaaagaac
     5221 ttgcagggac agtaagcgga aaggtgctga taaaggtagg cgacaacata actactgacc
     5281 atattatgcc atcaaatgcc aagctgcttc ctttcagatc aaatatccct cacctgtcgc
     5341 agtattgcct gacaccctgt gaccctgaat tcccaaaacg ggccagggaa aatgggggag
     5401 gctttattat aggaggatct aactatggtc agggttcaag tcgtgaacat gctgcattag
     5461 taccactata cctgggtgta aaaggtgtaa tagcaaaatc cttcgcaaga atacacatgg
     5521 caaaccttat taattctgga atattgccaa tgacttttgt agaggaaaag gattatgaaa
     5581 atatagatat ggacgatgat cttgttcttg aaaacgcgag agaacaggta aaaaacggtc
     5641 ctgtactatt aattaccaat aagacaaaag gcaaagagat caaggtaaga gttgcattat
     5701 ctgaaagaca gatagaaatc atacttgccg gaggattatt gaattataca aagaaccgga
     5761 attaaaataa tatgattata gtatagaaac tcatattatt atggtacaga aactcatttg
     5821 aagataatgg aggaccagcc ttatgctaag atttgtaaac aatggtgagg acagaactgc
     5881 tacttcattg ggtgctaaaa tatttaaaca acttcaaaat gatatattaa acgggaaata
     5941 tgaaccggga gatattttaa cagaaatgaa gttgtccgaa gaacttggcg tcagtagaac
     6001 ccccatacgt gaagcgttaa agcaactgga gcttgaagga cttgtaaaat caacacctgg
     6061 aagaggggtt gttgtacagg gaataacaga taaggatatt gaagatattt atactataag
     6121 gatgatgata gaaggtcttg ctgccaggtg ggcagctgaa aaaattactc ccggagaact
     6181 tgaagagctt aaagaaatac ttgagtttga agagttttat acgttaaaag gaggcggtac
     6241 gggaaatcta ttaaagctgg attccagatt tcatgattta gtttttaaag caagtaaaag
     6301 caggcctctt atgcatatat taagtacttt tcatcattat gtgcaaagtg caagaagtgc
     6361 ttcccttgaa actcccggta gagcgcagaa ggtttttgaa gaacataggg ctattttgca
     6421 ggctataaat gcaagagacc cagaccttgc tgaaaaactc acaacggaac atgtaaaaaa
     6481 tgcaaatatt aaccttatga aaaaaagaag tgagcaggaa cataagcagg aataaaaaag
     6541 gtggaattcg tatgagcgat aaaatagcag atagagaaat taaagacatg attgaagact
     6601 taagtatgct ggcaaaaagg aataattata taaatcctga actatatagc aggtataatg
     6661 taaaaatggg attgagaaat agtgatggta ctggtgtact tgtaggtctt actgaaattg
     6721 gtgatgttca tgcatatata atggatgaaa atgaaaaagt acctgttgat ggaagactta
     6781 tctatagagg tatcgatatt tatgaccttg ttgctggatt tcagaatgag aaaaggttcg
     6841 gctttgagga atgctgttac cttttattgt ttggacagct tccaacccaa agacaactgg
     6901 aagaattcaa gtggttgctg gctgagcata gaaaccttcc caacggattt accagagact
     6961 tgattttaaa agctccaagc aaagatatta tgaataagct ggcaagaaca gtcctttcag
     7021 catattcata tgatgaaaat cctgatgata caagtattag caatgtggta aggcaaagta
     7081 ttgagttaat tgccagcttg cctactctgg cagcctatgg ttatcaggca cttgctcatt
     7141 attatgaaga aaagagtctt tatattcata acccgcgtca tgatttaagt actgccgaaa
     7201 atatacttca tatgataaga ccggatagca agtatacaca aagtgaagca gagattctcg
     7261 accttgctct tgtgctgcat gctgaacatg gaggaggaaa taactctaca tttgcaatcc
     7321 atgtggtaac ttctacagga acagatacct attcagctat tgcagccggc ctcggttctc
     7381 tcaagggacc taaacacggc ggagctaata ttaaagttat gcaaatgatg gaaaatataa
     7441 aggataatat taaagactgg gaagatgatg atgaattaac agcctatttg gaaaaaatca
     7501 tgaataaaga tgcttttgac aagtcaggac taatatatgg aatgggccat gcagtatata
     7561 ccctttctga tccaagggct gtattgctta agaaaaaagc tgaaggactt gcagcagaac
     7621 agggattcat gaaagaattc aagctttttg aaagagttga aaaactatcg ccaaaagtat
     7681 ttcaaaaggc taaaaacact gataagataa tatcagccaa tgtggacttt tattccggct
     7741 ttgtttataa aatgcttggt atacctatgg agctttacac accaatattc gccattgcaa
     7801 gagttgcagg ctggtgtgct catagattgg aagaaattat tgatggcggt aaaattatca
     7861 ggcctgctta caaaaatgta ggagaaaagc aggattatat ccctttaagt gaaagataaa
     7921 acatttgcat gtattttgca taagattttt tacaaaaaat aaattgcacg tgcagctaaa
     7981 atgaatttaa gcccataaaa acttttttat aaaaaaagtt ttgttcctgc aagggcgaat
     8041 tcatttcgca cgcagaagga atgaggctta gtttgaaaga aattgtcaaa tttttgaaaa
     8101 actaatgcgc atgtgcatat tttttgcaca ggattataaa aaaataaatt gcacgcgcga
     8161 ctgaaatgaa tttaagccca taaaaacttt ttcataaaaa agttttgttc ctgcaagggc
     8221 gaattcattt cgcacgtagc aggaatggag cttaatatga ataaaaaagt caataccata
     8281 tgctacatgc tgatgctttt tctcggtata tacctggcta tgtaccagaa tagtataaac
     8341 tatattaccg aaggtgtttt aaataaaagt acaattatgg gtgttcttgt tgctctacat
     8401 ttcataggaa taataattgc tcctgtgaca tttggtgaag caggggatag gattggtaaa
     8461 aagaaagttt taactattgc ttttgcaaca attctttttg gtatatccat tgtatttgtt
     8521 tctaaatcga ttattttagt tgctgttggc atattattta taggatgcgg atttggagcc
     8581 attgagagcc ttggaagcag tatccttgca gatgaaaacc cttatgacac aaatagggtt
     8641 ataaatattt cgcaggtatt tttcagtgca ggagctgtaa tgggtcctct tatatctttg
     8701 aaaattgttg aaatatccgg cagctggaag ataagctatt taatagcatt tatatttttt
     8761 gcgtctatgc tcgttgctac ctcgaaaact gattttggag gcgtaaccgg taccggtacc
     8821 gatagtatca ccagtactgg tgctgttagt agcgccggta ttggtactga tgctgctgct
     8881 tgtaaagata ccggtagtgt tgccgttaca accaagaaaa caggccttta ctcttcaagg
     8941 ttgcttaaag acaggtattt ccaaatactt tgcctttcaa tattcctata tgttggtata
     9001 gagggggcct catctttttg gattacaaca tacttccgtg atgtgcttgg tgcagagaag
     9061 cttggttcct acgcactgtc cggcttctgg ggaagcatga ttgtaggacg atacataggc
     9121 agcaagcttg aaagtaaaag cagaattgtt atgaatataa gccttgttat atcaattgtt
     9181 tcaataactt cagggctatt aatcaagagt accatttccg gtcttgtaag ctttacttta
     9241 cttgggtttg gatttgcagt aatatggcct gtgctgatga caatggctgc aaaacgttat
     9301 cctgaaaaaa caggaactgc tatgggcatt atgatgacca tatcggcagt tggaggaatg
     9361 ataatacctt ttattatggg aatactgggg gatgccttga gcatttcagc aggactttcg
     9421 gtggtaccgg catccgcact ccttataatc ttattgcagt ttaaagcagt aacggttgag
     9481 tagatttgag caggttttac tgcaataata ggaatggttc ccgaaattat ggggatatct
     9541 acggttaaat gcagacatca ttaagtcatt tctaccagtt atttcttact atatatctct
     9601 tgttaattat ttctaataag ttattttcgg atagtgaagt tatcttcaag caaagcccag
     9661 ttttgaggga agggataccc taaagcccaa tagctgatac cttttaaatt ataggccttt
     9721 acagtgtcaa attttgcctg agcgctgcga gcgtcttcaa accatacctc gtgtgctctt
     9781 ccttgagcat ctatatacct ataaaaaggt gattgtgcta tagaattata ttgaatagcc
     9841 gcaccatacc gtattgctct caaaacagct tcctgacaac tgaatgtttc agcttcctgc
     9901 cccggaacaa aaggaaggag ccaatctctt gcatatattt gaaagccaaa aaagatcttt
     9961 tctcggggaa ttactgtaac agcataatca agaacacgcc ggatttgatt taatggtgaa
    10021 attggctgag gaggaccttt acgatacccc cattcatatg tcattaagat aacaaaatca
    10081 acaatcctgc catgagctgg ataatcatgg gctacataca ataatcctgc ctgctctggg
    10141 cctgtttttg gagccactgc cgtagatacg aaatagcctt ccggatgcaa acgggctact
    10201 gttttttcga gaaagtgatt ataagcttca cgatcctctg ggagcacatt ttcaaagtca
    10261 acgtttagcc cctggtaccc tttagctttc attgtattta aaatattggc aagtaaactc
    10321 tcaataattt gcgggttgtt taatacaata tgtgcaagat tttcgcctac agttgttgct
    10381 gtaaagtttg taatagccat cataggtata gcattctcag ctttggcggc ttgaacggcc
    10441 ggaatatcat taattgactg cagacttcca tcttctctaa ttaaataagc aaatgggctt
    10501 aaataggtta agtctttgcc atgttttctg acaattggta cagcttcttc tcccagaaaa
    10561 taaatataac cattaacatc tatgacaggt ctttgttttc gcggaataac cagtacagta
    10621 cctgggtata taagatttgg attttctata ttatttactt tcatcaggct ttgtaaggta
    10681 actccatatc tttgggctat tatccatagt gtttccccag gctgaacacg atggcggcgt
    10741 gctggaatat acaataccat acccggatat atattgttgg gatttgtaat ctgattactt
    10801 tgaacaattg cttccacggt agtcccatag atttgagcaa ttctccatag tgattctccc
    10861 ggtttcacag tataaaaaac atcttctgtt ggtatgacta atgcctggcc aactgcaagc
    10921 tgattgggat tgggaagctc attaatatcc actattgctg caatggaaac acggtactga
    10981 tttgctattg cccataatga ttctccaggc ctcacaacat gaatcaacat aaaaatcaac
    11041 cttcattatt ttctttttac taatagtata tgccgggata cagttatggt gtttccgatt
    11101 catgcaagtt tcttaactga ttctctaata accagattat ccgaatctac agttatactt
    11161 tctgtagatt taccttctat tttatcaata ataagctcca tggctagtgt tcccatttta
    11221 aatttgtcaa tcctgattgt agtaagcttt ggttcaatgt aattggccag aaagatatca
    11281 tcgatactaa taaatgaaat atcttcaggt accttatatc ccgaatcttt tgcacatttt
    11341 attgccccta tggcaaacat atctgtagta cagaaaacag ctgttggttt aaatttactc
    11401 tttaatattg ctttcatgca gttatatgct gaatcttcat taatggcatt tacctgtatc
    11461 caagatggct gaaccgacac ttgggcctgt tcaagtgctc tcttgtaacc ttcaaaagta
    11521 tgcaaataga aattaggaac aaagctggaa cttatatacg caatatcctt atgcccattg
    11581 ctggtaagat atctgacggc agtgtaagca gcaagttcat aatctgcatt gacagatgta
    11641 atatcactaa aggaagctcc tgagctggta gaattattta ggatgtcagg tttttctgag
    11701 ctagcagaat tctctgagtt atgggcatct gcaatcacat aaggaatgtc taatttgttt
    11761 atttcattca atataatatc ttctgtatcc tgaaaaaaga caattccgtc agtgtcctca
    11821 tttttaataa tgtccggcaa tattgctctc ccattttcaa ttgaaatatc agtaaaaaca
    11881 atattataac catattcttt gcttttttcc aaaagtcctt ttgcaatttc aaggtaaaac
    11941 aagtcatcaa aaggcgagga tgttttacgg attacaatac tgatattgaa acttttatta
    12001 agtaaaagcc tcctggaatt aagactgggc tgaaaattag tcctttcaag aacttctcta
    12061 actatttttc ttgtttcctc gctaacccct tctctattat ttataacgaa agatactgcg
    12121 gttggtgata caccagccat ttttgctatt tctttaattg taagttttgc catatttgct
    12181 cctttgtttt ctaatatgtt ttagattgtg taagcataga agaaaagcta ctgcactaaa
    12241 acattttata cgttgtacca aaacgtttta tataaataat attttaacat ataattttag
    12301 aagtgcaaag gggattttat tggcagggga tttctatgga gtgtaacaac actggacata
    12361 gcaaatgggg tcttttgctc gtttatcata atataatttc atgtaaccat tatatcacaa
    12421 catacagcaa actttttttg ttatttgaca taaaaaaagc cgctactatg cggttttggt
    12481 gatatttatt tagttgttaa gtgtcgaaaa tccgtgcata gaaaatcatc gacccaagac
    12541 tcaaaaaatc cgcaatctga ctgcaatttg tggtataata ttataaaagg agttgattgt
    12601 atgtctccag aattgcatat tcggcggctg gcagcagtaa agctcgccac cgcagtgaat
    12661 aaaatagaag gcgtccctgt ctcgactcaa gccaaaacgc tctcagctaa gtgggcgcgg
    12721 ggtgagattt caggtgcgga aatgaaagct acccttattg caaagcataa aaagggctaa
    12781 agggggttgc ttatgaatga cccgtatctc tatgaaggca gctctgttct gcgtaattta
    12841 ctgaatattc atgacgaaaa ggaactggat attgctgaag cggaactctc gcaagccaat
    12901 atgatgctac tttatgagca aggatttgat gacttttcca cttataagcc tgtggcacat
    12961 gaataccgag ctgacgatgg agaagataaa acgtagttta tattacttta ccaacaatca
    13021 ccggttgtgg gttcaattcc taccacaacg ttttatatag ataatactat aacatagtat
    13081 ctattatggt gcaaagggaa atttatttct tataacctat tcccaataat atcttctgaa
    13141 ttttctaagg gcttcgcata aagcatctac attttcagga ggtgttgggg gatatatccc
    13201 tgctataaac tccaagccac cctgaggact accaagcttt cttacttcct cctcaataag
    13261 ctcatgtatt tccttcctgg taccaaacgg tattattttc tgcctgtcta tatcaaggcg
    13321 tatacatgcc ttgcctttta tttctcgggc gatattatct atcccattac acaaatcctg
    13381 agggtttatt atgtcaactc ctgcttcgat aagatcattt gctatttcca taatatatcc
    13441 gtcactgtga aaagctacaa gggtgttctt ctctctacac ggtctaaaca gctttttata
    13501 tgagggcagt atccattttc tgaacatatc agggctaatg acaagagaat tctgcatacc
    13561 aaggtcttcg ggtatttcca ttatatccac acccatatcg aggtaccttt ctacaatttt
    13621 tatgttatgt tggtcaattt catcaataag tttgacaagc aacggttcat cagtatacat
    13681 atcaaacatt gcattttcaa accctctaag gtattggagc ctaaggaaaa gaaaaccatg
    13741 agccagtccg cctgcagtga gattgttatt tgatttattc aattcaatat tttttctttc
    13801 cctgtcccaa tttctaatac ttctatcagc gtggatatca ggatcaggta ccacataact
    13861 ttgaaagttt tcccagcttg ataatggagc atttctaaca acaccttcaa gaccgtttac
    13921 tggtgattcc catatacatc cccaggaatc tttgaaaggc tgcccttttg tataagcatt
    13981 tccaaaatta aaattcttat aatccctcca gcctttttta acataaggga aaaaatcagg
    14041 atgtctcaga gccacttcct caatgtcata tcttagttgg tcccaggaag cattgcttat
    14101 gtggacggaa gacggtatcc attcgggata ttgaaacctg gcattccgaa tatagttttc
    14161 tcgtgctgtt aacatgtttc atcactccta ctcaatcatt ataacgagat gataaatgtt
    14221 ttccacttct aattgtatta tcaaaacaat aacagtacta ctaatatcgt aggattgtac
    14281 catattatac aattggaaag gaatacaatt atttacaaac cgtggcaaac cgtaacaagc
    14341 tgtgagtgca cccatatgtc atatatgtat tgatgtataa ggagccttct gatacaattt
    14401 atatgtatgt taatactatg ttacatgtat gtttatgtta gaggtgaaat acttgagcgg
    14461 aaaaagcaaa tccagcaaag atatattctt aagagaacga ttaatgataa atttttccca
    14521 tactggaatt aacaacctca tcactttggg aagaataaac tatcttaacg ctcataaacc
    14581 attggacaaa catactcaca aggattccat ggaaatatgc tatctcgctc ggggcagaca
    14641 aacatattcg gtaggtgata agccttacac tcttaaaggt ggagatatat ttattacttt
    14701 cccggatgaa gaacatagca cgggaatcaa tgtagaagaa aggtctatct tgtattattt
    14761 gatttttgat attaagaaca atctggataa tttcctgggc tacaaaggtg aagaaggcag
    14821 agaacttgct ttggctttga ataacatgag cagagtaaac aggcatttcg caggaaatag
    14881 tgaaattaga agcatactgg atgaagtatt tttaaccttt tattctgcta aaaaatttaa
    14941 gaaaatagta atgcaagggc ttataagcaa gttgtttatt agaattattg aacttgaaaa
    15001 taacagaaaa aacagctttc ctgaagatat atctaaaacg attaattata taaatgataa
    15061 tatccagcat gagttacggc ttgataaatt agcatcagat gcaaaccttt cattatctcg
    15121 ctttaaacaa aaatttaaag aagcagcagg tattccaccc ggagaatata taaaaagagc
    15181 aaaagtcgat ttctcaaagc atttactaaa agacagcgac agcacaataa ctgaaatttc
    15241 ataccagctt ggtttctcat cgagccagta ctatgcgatg gtatttaaga aatttaaggg
    15301 cataacacca aaggatttcc ggataagagt aaggaaagat agtaattatt tggggctttg
    15361 atggatggat ttgccaagta tttcttcata tttagaaaat gcacccctgt ctataaatcc
    15421 tattgacaat tagtacttga caatatataa tttacttgac taaaacgttt ttgtaaattg
    15481 agttatgcat cagaaattat ttcttttata actggggtta aattagcata atgggaattt
    15541 cttttagcag aatagtaata ttttcgtaac taaaacatat tatcaagttt agcagtgaaa
    15601 gattatttga atggcagaag atgctacaaa gggggaatta taatgcgcag gttaaaagca
    15661 ggtattgtag gaaccggcta cataggtata agtcatatag aaacaatcag aagaataggc
    15721 tttgccgagc ttgttgcagt tgctgatgta aactatgaca tggcaagccg gcgggctaag
    15781 gagtactata taccaaagtg ctatggcaca ttggatgaac tgcttgcaga taaggaaatt
    15841 gatgtaattc ataattgcac accaaacaat ctgcatcttg aagtaaacga gaagataata
    15901 aaagcaggaa aacatatttt ttccgagaaa cctctggcaa gaaacagcga agaatcctta
    15961 aaacttttac tactgcttaa tgagaataaa aacataattc atggaatgaa ttacaactac
    16021 agaatgaatc ctttagttca ggaaatgaag agcaaagtaa aaaatggtga aattggtcag
    16081 cctaaattag tgcatggctc atatcttcaa gactggctgc ttttcgatac agattacaat
    16141 tggcggatag agcctgaaat atgcggcccg tcaagatgca ttgctgatat tgggacacat
    16201 tggatggacg cagtacaaac aataattgga gctaagattg ttgaacttac tgccgacctg
    16261 gtaacaacca tccctataag gaagaagcca aaaggtcagg ttgaaacctt ttctattaca
    16321 ggcggtactg agtatgaaga agtagaagtt aaaactgaag actacgggtc agttattttt
    16381 aaaatggaca acggtgttag aggagtattc tgtgcatcgg aagtcagtgc aggaagaaaa
    16441 tgctatttaa attttgaaat agacggaaca aggtcatcaa tgtactggaa tcaggaaatg
    16501 gccgatcaaa tgtggatggg cttcagggaa aaagataaca gcctggttat gagaaatcca
    16561 aacctcatga cacctgaagc aaaacaatat acctaccttg ctgcgggaca tcctgaggga
    16621 tggaacgatg cgatgcgcaa taatgtatac agtttctata agtatattgc tgatggtaaa
    16681 accatgggca aagacccttg tgatttcgcc acctttgatg agggacatta cttgattaaa
    16741 ctaactgaag caatattaga aagcaacaag acaaggcaat gggtgaaagt agagtaaatt
    16801 gctgacggta aggagcaaag aagtagcatc ttaacggatg gaaatcccaa tatttcctga
    16861 agctgcagct aactttgcat aataaaattt gcgttatata aaaaattaaa ttatcaagca
    16921 agggaggatt tttatatgaa gtctcagttt aaattttctg taggaccctg gaatgtccat
    16981 gaaggagctg atgcatttgg accggaggta agaaagacta ttgaatttgc taagaaagtt
    17041 gaaaaattta aagaaatcgg atttgatgca gtacaatttc atgatgatga tgcagtgccg
    17101 gatatgaata atttaacgga tggagaaata aaagcaaaag ccggggaagt aaagaagatc
    17161 cttgacaata atggaatggc agcggaattt gttgcgccca ggttatggat ggatcccagg
    17221 acagcagatg gaggtttcac ttccaacagt aaagaagaca gagagttcgc catgtggaga
    17281 gccttcaggt ctattgatat agcaaatgaa ctgggatgta acaaaatagt gttatggcta
    17341 gcaagagaag gcaccctgtg ctatgaaagc aaaaatcctg tagaaggtgt gaaaaggtta
    17401 attgaagcta ttaataaaat gcttgcctat gacaaaaata ttaaaatttt aattgaacca
    17461 aaaccaaacg agccaattga cagaagtttt tgccctacta taggacatgt aatgggagtg
    17521 tctgcagcta caaatgaccc ttccagagtg gggggattgc tcgaatctgc tcatgcaata
    17581 ttagcaggac ttgaccctgc aaatgaaatt gcttttggac ttgcatttgg gaagctatgg
    17641 ggagtacatc taaatgacca aaacagcata agatatgatc aggacaaggc atttggggta
    17701 gaaaacctcc gtcaggcctt caaccagata aaagtactta tagaaaacaa ttatggtaaa
    17761 aacggagagt acatagggct tgatgtgaaa gcaatgagaa cacagagaga tgaagtgtgc
    17821 tacaggcatc ttgaaaacag tttgaaaata gcaaagatgc ttgaagagaa ggcaagaaaa
    17881 tttgattata gcttccagaa gaaatgtgtt gaggaaagag attatgaggc acttgagatg
    17941 tatgtgatgg agttattgat gaactgatat attgatttaa taaaaggtag cagccagtat
    18001 gtggttatat ttgaaaatct cttcccctag gggaagagat ttagtttaat atgaatgtag
    18061 catatctgtc cgaacagtta gttaattgaa ttgcataaaa gtttatgtta ctataatagt
    18121 agttataata gttgctgtaa tagtggtcga gaagaaggtg ccaatcatgt atactaagtt
    18181 acaattagat atgattataa acgaaataac taatagtgtt agagatattt tgagcagtaa
    18241 gttctataaa attatcttat atggttcgta tgcaagaggt gactacgata atgaatcaga
    18301 tatagatatt atgatacttg cggatttgcg gaacgatgaa ataagcgcgt tgagaaaaaa
    18361 attaacaaag ttgcgagtaa agtcggttta gagcatgata tcatggtttc tatattgaaa
    18421 aagattggta gggtggaaaa aagatggggt gaaattatga caatattgtg tcaaaaatat
    18481 acaaccaacc gggacagacc ttttattatt agtagcggcc ggggcactta tttcccttat
    18541 cattggcatg acgaaataga actgctatat gttttggaag gaagtatgac ttttgttata
    18601 gggcctaaag cttataatat aaataaaaga gatattgtaa tagtaaaaag cggagaagtc
    18661 catcgatttg aagctaaagt tgcatgcagt catctttttg ttgaatttgg tcactatctg
    18721 gatactgatt tatttaagtt acctatatta atttatcccg ggatgcttag tgttttaagg
    18781 gctcaaaatg ggagccggga agatttgaac caattgcatt caaaagttga aagccttatg
    18841 ctggacatgt taaccgaata taaccaaaaa actgatggct gggaattttc tgttaaagca
    18901 tctctatacc ggataattgt tttactcacc agagcatttt atgacaaaat agaaatagaa
    18961 gtggaacata aaaatattac acgaggttat actgagatac tccaggaggt atttgaatat
    19021 atagagggtc attacaacga aaatataact atagaggatg tttcagaaat cgcaaatttc
    19081 agcaggcact attttaataa attcttcaag aaagcaaccg gcaaaacttt tgcaagctac
    19141 ataaatgata tcaggctgga aaaggctcag gcctttctta agtcctcaaa tcaaaatatt
    19201 acaaacattg catatgatgt tgggttcaac agcgttaaaa cttttaacag gctttttaag
    19261 gaaagctttg gatgtacacc aagcgagtat agaggttcag tgagggcatt gtaatccccc
    19321 actgaacaag ggtattccta taatttgcac tactataaag ctgctgctga ttttctgtaa
    19381 gcctcagcct tcttctcaat atcagcttca gaccaatccg ggctcattcc aacaatgaca
    19441 gttctgctta atatgtcaag agcattagga cacatatcat atgaataatt catatttaga
    19501 tctttatttt gtagcagctt gaacggatct aatacagggt ggtgtgatcc cctttttttc
    19561 attattggct cccagtcgaa atagacatgc ttcttgctat ctataaggac accggaattt
    19621 acaccttcac tagcagcaaa cttcctggca gcattttcgc tctgcaggag gaaaccaaca
    19681 ttagaaccgc aatcgccctt gatatcattt gacctaataa acttaatgtt cttctcgcct
    19741 tcaagacgtg acataaatgc cttctttacc ttccgaagat ctgaaagtat atcatccagg
    19801 cgttgaagct gaactcttaa tatggctcct gcaatttcat tggaacgtaa ttgagaacct
    19861 atgaagatag gagtttttag accaccagca tgaggtctga acgctgtacc accatcatga
    19921 tatattaatg ccttttcgta gatatattca tcatttgtgg ttacggctcc accttctcct
    19981 gcagtgatta ttttgaaata attaaagcta aaagcacctg catcaccaat actgcccagc
    20041 cttctgccct tatagctccc gccatccgcc tggcagcaat cctctacaat ctgaaggtta
    20101 tgtttttcag ctatttcctt aattctatcc atattgcagg gaagtcccag catatgtaca
    20161 ggaataatag ctttagtata ctttgaaatc tttttctcta catcatcagg gttaattgtc
    20221 aaactatcat ctacctctgc gataactggt attgcaccta cggccacaac tgccagggca
    20281 gtagccataa aagtatatgc cggaactata atttcatccc ccggacctgc gcctatacca
    20341 acaagtgctg agataagagc tccggtacca cctgttaatg caagactgta cttggtacct
    20401 attttttctg ccagctcttt ttcaaaactt gccacttcct tattaccttc gttaaatcta
    20461 aatagttgac ggcttttaat taccttttca actgctctaa cttcttcatc tccaatcctg
    20521 tacataaaaa tgcaccccca tcttcatatt tgcaattatt tttttgctca gtttgtttgg
    20581 aatgtggata tgcaggttaa agcatgccta aatgcacctg cattttattc atgtcacctc
    20641 aatattatgc ttgtattgcg cctgtgtcgc gcctgtatcg cccttacttg tattgtgctt
    20701 atgccgtgcc tgtacattac ctatataatt tacgatttga aagagttaat gaagaattta
    20761 caaatcgtaa attacatcaa ggtaacagaa gcagatttta aatgttttaa aacctgttct
    20821 atatagttca tgtttataaa tcgtgcctac attaagaata gtacaaatta atataactat
    20881 atagtcaagt tggagcattt gtttctcaaa aatagctttt ttgttctact attattttac
    20941 atggctattt ttgggtataa tatcgctatt tttgactata gttaaaatgc ataacaactt
    21001 ataataaata tatactcaat ttgtgcaatt ataaaaaaat aatactaatt tatccatttg
    21061 tgtaaggaat gtgtaattta aacatgccgt atcgctacaa caagtttatg aatttatgta
    21121 cacgctcttg taagtgcaag caaagggtta gtaatatgta atatagaaca cagtaatata
    21181 taataccttt gaatataatg tcgcaggagg gatgtaataa gtgaactttt ttgattgtaa
    21241 tgtctgctat ggttacgacg ttagaaacaa tgtgatgtac ccggtcaata ctataaatga
    21301 gttaagctgc catatggagc aggcaggtgt tggcaaggca atggttagcc gggtagagca
    21361 gtatatagct ggtacacctt ctacaaacca gatactggca gaggacacag caaatcatca
    21421 aaatatgtat ggaatatggg gtctattacc atctcacact catgaaattc ctgagggtga
    21481 atttatgcta tcaattatga aggaaaagag aatctttgga tggcgtctta tccctaacat
    21541 cggaaggtat ctaattaagg attttgtgct aagggattgg tttaaacttg ctatagaacg
    21601 gaaaatacca atatttatta atacggctca tgggaccagt tttgaagcaa cagttgaaat
    21661 cttgaaagca tttcctgagt taactgttat attaacatac ccccaaatat ggcctaatga
    21721 caggtattta aggccctttg ttgcagaata cccaaatgtt tatcttgact tatcctattg
    21781 cattaccgat gggggcatag aatcttttgt taatgaatat ggggctggcc gattgcttta
    21841 tggaagtgga tttccatact gttatttcgg ggcaaatatg ttaatgataa agcacgctga
    21901 tatttcggag gatgataagg ctgctattgc aggcggcaat atgaccggaa ttataaaggg
    21961 ggtgttgttg tgattaactc taaatcggaa ctagctcaag gattttggga aaaagggaaa
    22021 ttaccggatt gtcctattat cgatttccat gctcatatgg gagataatgg cggtatattc
    22081 ctgcccaaga gtacacctga tgcaatgatc aatactatgg atgcctgtaa tactattctt
    22141 acatgtttct gcggacatga agctctcttt gtgccgtcca ttggtgaaaa acttgatatt
    22201 gcagctgtta aaaagtatcc agataaattt aaagcatatc atgctgtggt tagccgttac
    22261 cttaaaccgg aggatgatat tgccagggta cgggaaaatc ctgatgtttt tataggtttt
    22321 aagttcctat gcgattacta tggggttcct cttagtgatt ccagacatga caagtattgg
    22381 gaatatgcta atgataacag tttattagta ttaacacata catggggagg cagcaatcat
    22441 aatggatgca aggaagcaga gaaagtatta agcaaatatc cggatttggt ttttattgca
    22501 ggtcactcat ttcatggcga atgggataaa gctgttgaac ttacgaagaa atatccaaat
    22561 ttatatcttg aattgactgc tgtattggat gatcggggac cattggatat gtttgttcaa
    22621 gagattggtt ctgaaaggat tctatttgga acggatcttc cctggttttc aacgcaccat
    22681 gggataggtg cagtgctttc tgctgaaatg acggacgaag accgacgaaa tatattttat
    22741 cgtaatggtg ttaaattgtt gaagaagttt tcttggtttg aatatatgtg gaaagataga
    22801 caaagttgca tccaataaat tacatgcgtt gcaggcagga tgcactgcaa ataatgaagt
    22861 gaaggagata tgtaatatgg gaaaagcaaa gtttgcatac actgtatggc agtggggcac
    22921 ggataataaa gaacagctaa ggcaggcact tgcagatata cgtgaagcag gtttcgaata
    22981 ctttgaaagt gtaaaagcca ccataaacgt ttttgataac gatgtttcgg gttttaaagc
    23041 gctttgtgag gaatatggag taaagcctgt tggattttat tttcatttta atggtgattg
    23101 ggaaaatgat gttggagatg ttaagcgaaa actgccattt atggcggcgt gcgatattca
    23161 ccgtatgaat gttcaagcga caggtgttgt tggcagaccc gcaacgaaag aagaactgga
    23221 ttataatata aaggcgataa acgccattgc taaattagca aaggaatatg atattgttcc
    23281 aaccatgcat aaccactaca atacaactgt tatgtatgaa agtgaaattg attatatgct
    23341 gcagaattta gatccggatt tagtagcttt tggtcctgat actgcacacc tagtagccgg
    23401 tcagtgtgac cctgtaaagg taataaaaaa atatgcagat cgtgttaaat ttactcactt
    23461 gaaagacatc accaatgaag ggatggaagc tgccggtatg caggccggcg tagaggtgta
    23521 taccaacttc cgcgagttgg gtgaaggtat agtagatttc ccatctatct ttaaaatcct
    23581 ggatgatgca ggctatgatg gcttcttaac tgctgagctg gacagaagta gatttgggaa
    23641 caaagaaagc gcaataatgt caatgaatta tttaaaagct aattataagc aggattgaat
    23701 aatttaggag tatggaacaa gtgtaattta atacacatta ttgattaagc cattgttatt
    23761 aggaggcgct tcttatgtct gaatcactag aagttggagt agggagagca gacattaccc
    23821 ctgcactagc ggtacaactt atgggctatc gtgttataag acgagagtcc atcaattttt
    23881 attggtttat ccaaattatt ttctgcatac tgaaaaactg gctgaaggat gttgtaagtg
    23941 atgaaatgca tatccatatc ttcatccagg gacaaatgta tttttcttct tcaagagata
    24001 ttccttcttt aatattatat agaagatact tttcaattga gattttaact ttcttatagt
    24061 atgtttttat gaagacccaa aattaatctt gacaccaatg attcagtaat gtataataaa
    24121 tttaactaaa acgtttttgt tgatcatttt tagtattatc aatcaaatca tgaatcgggt
    24181 agactaatgc aaaataaaac cgacaattta tcctatctaa aacgaattat gagggggaat
    24241 atcaaaatgg caggaccagg atcctattta tttggtgagg aagaaagaaa tgaactgctt
    24301 gatgtaatgg aatcaggcta cttatttaga tatggagatg aggaaaatcc ttgttttaaa
    24361 cgaaaagttg caacttttga aaaggagttt gctgaatatt caggtataaa tcattgtgtg
    24421 gctgttagca gtggcacagg ggctcttatg tgctgtcttg cagctatagg gataggtccc
    24481 ggtgacgaag taatagttcc cggttatact tttatagcaa gtatttcatc aattatttta
    24541 tcgcatgcaa tacctattct tgcagaaata aacgaatctt taactattga tcccgaagat
    24601 attaaaaaga aaattactgg caagacaaaa gctataatgc ctgtacatat gcttggaaat
    24661 ccttgcgata tggacgagat aataaaaatt gcagaagaat acaatctcta cgtaatagaa
    24721 gattgctgcc aagctgcagg tgcaagctat aaaggcagga aagcaggaac tattgggcat
    24781 atgggagcat tctctctcaa tatatttaaa actataacat caggtgatgg tggcatggtt
    24841 attacaaatg attacgacct atatgaaaga gcatttgctt tccatgacca gggacataaa
    24901 ccgaatagga tgggtgttga agtaggaaaa agaagtattg tgggcatgaa tatgaggtta
    24961 aatgagctgt caggtgcagt tgcccttgcc caattacgaa aacttgatac tatactcgca
    25021 ttgttacatg aaaaaaagaa gaaattaaag gattctatag aagcttacat aaaagatatt
    25081 ccagatgttt attttagaaa attaaatgat gaaggtgaat gcgcaactct tatgacactt
    25141 ctatttaaag acaaacaaaa agctgatttg tttgctgaaa aaataggtgc aaagactata
    25201 gttcattcag gatggcatgt ttataataac atggagcaga tattaggtaa aaagacagca
    25261 actgattatg gatgtccata tgtatgtgaa gcttatggaa gggaaattga gtataaagct
    25321 catatgttgc cgataactga taacatactt gaaagagcag ttaatatcag tgttggagta
    25381 gtagacaaag gactagggtc accatttggt attaatatta cttcaacaga tgatgaaata
    25441 gctgaagttg cttgcgaaat aattaccctt ttaaaacaag tgacactacg tacataaaat
    25501 attatagtgg agttggaggg agatgaatga tatgtttgct ctgaattcaa cggatgtaag
    25561 aaatgaatgg agcctttagc tgatcttgtt attttaccga cttttcataa ctgctgggcg
    25621 atatacctgt tatgttttta aagcttcttg taaaatgatg aatgcttgta tagcataact
    25681 tttccgaaat ttgagtaaaa tttaaattac cttgtcggat taattttttt gcttctgcta
    25741 ttttaaagtt tatgtaacaa tctataatgc ttttgccggt tgcttctttg aaaagatgac
    25801 aaatatatga tttgcttaaa ttgaactttt cacatatatc cttaagggat atcctttcat
    25861 aaatattgtc caggagatac ttttcaattg aatcaacaag ggcattctct gcatttttct
    25921 ttgcgtagcg acttattcga ccgttgcgct cattattctg attactgcgc atcagagcga
    25981 tgagaaactg ctcaagatag cttattacta attgttgtga tccgatttct gaattcttga
    26041 tatccaacag ttttttgtaa agattattgg gaatagaaaa agatgttttc tgcgcttcct
    26101 ccaagagaag ggatagtatt ttcttatgct gactatccac agtgaaaatt ttattttcga
    26161 agaacttcat agcctcgcct ttgactgaaa aagttattac catcacattg tgagggtcct
    26221 ttttgtttga agccagagta tgaaattcca taggcttatg gaagattaca tctccttgct
    26281 ttataatata tcctgcatta tctgcaataa cggagatttc tccgctatca acatatacca
    26341 tttcccaaaa gttatgacgc tctcccgtaa agtaaaaatc cttgctgtgt tcaaaataat
    26401 aaaatgtata aagtcctaaa atatcaatgt ctttcttaaa agttttcagt tccaaaccca
    26461 ttttctttat ctccgtggaa tatatgttaa attttacaca caaaaaagca aagacaaaat
    26521 acagttgcta ttatatcata aacataaaat aacacaagga gtgaacatat tccatatgaa
    26581 aatttataaa cctgttttat aaacctaaga aaaagtgcga aaaggtaaaa agatgaaaag
    26641 atacatttaa aacattaaaa gattattgaa aagaattggt gtaatacacg attatagagt
    26701 taattactac agcgaattat ataacacagc gaattatata acgcaaaagt gattctacaa
    26761 ggagtaaatt gagtttttac ttatttactg ccaagtaaca tgaaccacaa aacttttata
    26821 atgttttttt atgcgttata tatagatacg gaggatttca atgcataata tagttttaat
    26881 aggatgcggt catatgggga cagcacattt ggaagatata tatttacgtg aagatatttg
    26941 tgtgtatggt gttgttgata ttatggaaga aaaagcaagg ctgactgcta aaaaatacaa
    27001 cgcaaaaagt tttagtacag attatatacg ctatatggag gatgaaaatg ttgatataat
    27061 tatttgcgct acatacccgt cgactcactt gcaaatatta aaggattgcg tgaaatataa
    27121 taagcacctg ctttgtgaaa agcctataac ggataattta gaggatggaa aagaatttgt
    27181 ttccattgtt aaaaatacca gtatcaaagt tctcatcgga tacatattac gatataatga
    27241 aacatacaaa aaggttgctg aaatgataga agaaggtgcg ataggaggtc ctattgtact
    27301 tagaatgacg caaaataatc acacagttga atgggaaaaa tatcttgcat tgataaaaga
    27361 aacatcacca ataatagatt gcggagtcca ttacattgat gtttgcaggt ggtttacagg
    27421 tgccgaagtc attgatgtgg gaggaatatc tttaacaacg gaaaaagatg ttccgaaagg
    27481 taaatacaac tatggaatga ttacaatgaa attatctgat ggcagtgtgg catattatga
    27541 agcgggctgg ggtaagacag tcgctgctga taatacaaaa gaatttatcg gtccaaaagg
    27601 cagaattaaa atagtacaaa gtcataatag attttttaac aaagaagaag gagacttgat
    27661 agagtactat aacgttgaaa ataaagagta taaaattata aatgttaatt gtaagagaag
    27721 acctacagga gctgaacttg acaatttaat aaatatgatt gaaaacaatg caccatctaa
    27781 tccatctatt gaggatgtgt ataaaagctt tgaaattgca ataaaggcgg atgatataat
    27841 aagaccggat atttgctttt aatagctttt gaaattttag agaacttaaa aaattaacga
    27901 attatataaa ttgaaaattt tgtaattaaa cagattgtta aatacgtaat aagtacaaat
    27961 gtttatataa tttggtaatt acagcagctt ttgctgctga gtagttacaa ctatattgta
    28021 atggatagga ggatttgtaa tatgaaggtt ggaataatcg gttttggcgg ccgtataagg
    28081 ggtatgttca aagctctcga aagacaggag ataggggata ttaccctatc tgcaattact
    28141 gatattagaa atgatgaaat taaagagctg ttaacaaaag aaggaaaaga tcctgaaaaa
    28201 gtcagcttct acgaagatcc tgacaaaatg atggataatg aagaacttga cggaataatg
    28261 ataggcacca gatgttcttt gcacacaaaa atggctgtta aggttcttga aaaaaacatt
    28321 ccattattcc ttgaaaagcc tgtagcaaca catatggatg atttcttcaa actgaaagaa
    28381 gcaggagaaa aaacaaaagc ccaggcagtg atttccttcc cattgcgtac atcgattatt
    28441 gcaacaactg tcaaagagat aatcgattca ggaaagattg gcacagttga acatgttcag
    28501 gctataaata atgtgcctta tgggagagta tatttccacc attggtatag ggatgagaat
    28561 gaaactcagg gattattcct gcaaaaagct actcatgact ttgactacat aaactattta
    28621 ctcggctcaa aacctgtaga aatttgcgca atgacgtcca agcagctttt taagggtgat
    28681 aagcctgctg gcttgtactg caaagattgt gatgaatatt acacctgtga tgaaagtcca
    28741 tatgttatta aaaagtacta taaagataat cctataggtg aaatgtgctg ttatgcaaaa
    28801 gatacaggaa atgaagattc gggaagtgca cttgtaagat ataataccgg gatgcatgta
    28861 agttactcac agaacttttt tgcacggcaa aaagcaggtg caagaggagc tagactattt
    28921 ggatttaaag gcacccttga atttgactgg tacactggag aaataaaagt attcatgcac
    28981 cacacgcctc gagttgacac atataaattt gatatgtccg gtggctccca tttcggtgga
    29041 gatgatgtgc ttgctgaaaa cttcataaaa gttatgcagg gaatagaaaa ctctattgca
    29101 ccactagaag caggtcttct tagtgctctc atgtgtataa aaggcagaca atctgccata
    29161 accggagaat ttaagaaaat tgatgaaatg tagtatactc ttttttaaat aatactctct
    29221 ttatagcagg atgactaaag tcattggtgc atttaattta tctgaagaaa atattggtaa
    29281 ggggcataag tggaggtgct gtagagcctt ttaatctgag tgaagggata gcaaaatcag
    29341 tgtttgaaca aaataattac aattctattg ttcatattat tttgtgagtt tgattttgcc
    29401 cgtagctgaa tttaaaggct ctaagcactg taacgatagc ttctggtaga tatttcttca
    29461 gatttgataa atgcacccta aagtcatcct gctatattat atttaccgca attaatgtat
    29521 aatgttatta ttggctctat actaccaatt gagttaaagg tgagatctta tgaatgaaaa
    29581 caggcaaaaa agaattagcg aaattgtcca caatgtatta tggtataatg agaatccagg
    29641 taaaaattat ctacagcgag tcatagcaat tgtagaaaaa gctctgggag ttaacataac
    29701 ctatataaac ataaaccgta atacggcttc agattcacgc ctggatattt tacgcaataa
    29761 tgctgttcac agcagcagtt attgcttatt tataaaagaa aagattaaag gacaaaagta
    29821 ttgttatata tgtaaaatga agggaataaa aaaatgcatg accttaatgg aagagtttga
    29881 tggtatgtgc ataatgggtg tgcacgatat tgtaaagcct gttataaagg atgatgaatt
    29941 gtttggtatt ctttacttga gtggaatacg ttatactccc gaagaatgta aagcaagaga
    30001 gttgataaat aagcattgta aattgcttgg cagatatgag gaggaaatgc ttaaggagta
    30061 tttgagacta acgcctgtga atattgaagg acttggggtt atggagaaat gcctggaaga
    30121 actgcgacaa atcattctgg atatattaaa tactatgaaa cctttaaatg tcggcgggaa
    30181 agaaatctcc ggtagttcgg gcgcttccgc ttcagataaa aaatggattt ataataccat
    30241 cttgccatat atagaagaga attataaaaa taatgtctct cttgaagaac tagcagagac
    30301 ttttttcata aattcacagt acctttgcag attatttaaa tctaaaaccg gtatgaattt
    30361 caaacagtat attacgcagc ttagaattga agaagccaaa gcaaaactta ccgtgccaat
    30421 taactcaata acggatattg ctctgtctac aggctttaac gatccaaatt attttagcag
    30481 agttttcaaa gataatgttg gaatgactcc gtcagaatat cgaaaatcag cgtgtccctg
    30541 tagtcaattt agtgctagtt gtagtcaaaa aattaaaggt tttatagatt aacttatgct
    30601 agtattagag caactgaaag caaaaaaatc agatattcat ctaaagtgaa caacctaata
    30661 aattcttgct taacatatgt tactttgcac ttgtattata tgtattaact aaattcgaga
    30721 tatatttata ataaaacggg gtgataatat tggaaaatca ttcaaaatat gatgtcatag
    30781 ttgccggcgg cggtcttgca gggactttat ctgcgattgc ttgtgcaaga gaaggcttga
    30841 aagtattgtt aatagaacga tacggttttc ttggaggtat ggctgtatca ggtcttgtca
    30901 atccttttat gccgtatttt tctaagtcaa agattgacgg gtcagaaatt ccggttaata
    30961 atgccggact ttttcagtcc ttattggata ggctttctga aaaagactca ctgaaaaacc
    31021 gttcgatatt taatgaacaa gtattgaaag ttatattgga cgaaatgatt ttagaaaaca
    31081 atgtagatgt acttttccat tcctttgtat tcgaaaccag gaaagaagga aacttaattg
    31141 caagcctaaa agttgtagga aagtccggtg tgagagaata tgaagctgat tacttcattg
    31201 atgccacagg ggatgctgat ttatcttatc ttagtggctg cgagacaaag cagggccggg
    31261 atgaggatgg gttgtgccag ccaatgacta cctgtttccg cataaagaaa tctgcttctg
    31321 cagaaaatga accaaagatg tccagagctg atataaataa gatatataac caggcgcaga
    31381 aagaagggaa aattaaaaat cctcgtgaaa atgtattgat ttttgatcat acatgtccgg
    31441 atgtagtcca tttcaactct accaggattg ttggaaaatc agcagtagat gctgaacagc
    31501 ttacagaggc ggaacttgag ggaagaaggc aggtctatga actgtattgc tttatgaggg
    31561 acaacattcc tgagtatagg gacagtgttc tccttgaaat tgcaccacag gttggaatca
    31621 gggaaagccg ccgtattgtt ggaaaatatg tacttaccgg tgaggatgtg ttgaactgtg
    31681 tacagtttga ggatgctatt gcaagagcct gctatgatat cgacgttcat aacccggcgg
    31741 gaacaggtac agttattaaa agaataaaag gcgactatta tacaatacca ttaagatctc
    31801 ttattcctaa tggcgcagac aatttagctg ttgcaggaag acctatttcc tgtgaccatg
    31861 aggcacattc ctcattgaga gttctgccaa tagcttcatc aataggagaa gcagcaggtt
    31921 gcgctgtagc tgcagcggca aaaagcaagc ttacactcga cagagttaac tatgaagatg
    31981 ttcagaatat gctttcaaaa tatggcgcaa tatattaaat aaaatctaaa aatgggaggg
    32041 tgcatttatt aaaagtgaag aaatatttac caggagctga cgttacgctg tctagagcct
    32101 ttaaattcag ctccaggcaa aattgaactc ataaaattgt ctgaaagaaa aataagaaac
    32161 aattttactc aaacaacaat tttgcttatc ttacactcgg attaaaaggc tctacagcag
    32221 cttcacttat gctccttacc aatattttct tcac
//
LOCUS       DAOSDD010000020        32124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_685_length_32124_cov_4.991362, whole genome shotgun sequence.
ACCESSION   DAOSDD010000020 DAOSDD010000000
VERSION     DAOSDD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 32124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32124
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_32124_cov_4.991362"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..232)
                     /locus_tag="PK733_03285"
     tRNA            complement(146..232)
                     /locus_tag="PK733_03285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Leu,seq:caa)
     gene            complement(353..955)
                     /locus_tag="PK733_03290"
     CDS             complement(353..955)
                     /locus_tag="PK733_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09598.1"
                     /translation="MSPFYTIFLIVGLIMLIVCTFLILEDRKKFINDMKQMEEKEKQL
                     SEIVMDAEYMVDELNKLSDFIIDRIEEKKNELDIKINNISTQINFIEEKAGELSEKLD
                     LVEKYGEDSRQDIDNSMYAIDIVESCKNNKIKDINDTNSGIDATSKGKIKQGAIKNFT
                     VNKKYEDVILLLEKGMNEAEIARTLNIGRGEVHLVLQLMM"
     gene            complement(979..2409)
                     /locus_tag="PK733_03295"
     CDS             complement(979..2409)
                     /locus_tag="PK733_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOJ09599.1"
                     /translation="MADINNENNNIKANEEGINKDTLVLVDVSSDKMSAFITLFAPEY
                     GQSHTVEGILEALKSYGVTFGVDREIIELAVKQNIFYKKIIAAKGLSPVNGKDGQVQF
                     QFDLNKNYKPIILENGKVDFRDLGLIESVRKGQVLCALVPPTQGIGGRDVLNSEIPAY
                     EGKTARLPVGKNVEILNDSNSLIASVDGQVSYSNGKISVYTSYEIKGDVDNSTGNINF
                     VGNVIVRGNVLSGFSVVAGGNIEIWGIVEGAEVISEGNIVIRRGVQGNNKAYIKSNGD
                     IVAKFIEQSKVEAMNDIKAETIMHSSVKCGGSLELGGRIGLLVGGSSAVCKELYAKVI
                     GSQMATLTEIEIGVNSDLRDAVMNIKSEINSIQNSLQKTEQVISILSKLESNNMLTED
                     KKGIYEKSLFTRDFYKSKLSQLNYDIEQIEKMISMQSAGKLRCLGSIHPGVKVTIGSS
                     TMYIKEELKYCTLFCDGVDIKVSPFV"
     gene            complement(2550..3308)
                     /locus_tag="PK733_03300"
     CDS             complement(2550..3308)
                     /locus_tag="PK733_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ09600.1"
                     /translation="MSSLEYNCNIWKQYADTKDILIRNKIIIKYLYLVKYIAGRMNIY
                     FGSNIEYDDLVSFGVFGLIDAIDKFDISKGVKFETYASLRIRGAIIDSIRELDWVPRS
                     LRQKNKDLERIYCELETKEGRPPTDYEVAENMGITVEEYNKLLNEVNLVSLVSLDEFL
                     EQNFQIDSEMLHINGDDMPEYCFEISELKDYLGESIEKLPEKEKLVISLYYFEELTLK
                     EIGAILKVSESRISQLHTKAILRLKGKLAGYIKS"
     gene            complement(3324..3809)
                     /locus_tag="PK733_03305"
     CDS             complement(3324..3809)
                     /locus_tag="PK733_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517991.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOJ09601.1"
                     /translation="MSNIINIGMAELNTSKHPGILVTQGLGSCVGIAMYDVKTKAIGL
                     AHIMLPLSPNSSSNINAAKYADTAINKMLHDMNRLGASIENITAKLAGGAQMFNFNET
                     SELMRIGERNVIAAKEKLSELGILVVAEDTGGNLVRTIEFYSENGGLMVKTLGIGTKW
                     I"
     gene            complement(3802..4272)
                     /locus_tag="PK733_03310"
     CDS             complement(3802..4272)
                     /locus_tag="PK733_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ09602.1"
                     /translation="MEEAVAVNSFKLLSFTLGTQEYGIDISFISTIVENNYSITRVPG
                     TSDCMKGVINLRGDIVPVMDLRTRINLPGVEDTQETKIIIVNMEEYSVGIKVDQVNKV
                     IEINKSTIEKVSGISGEDSSDFFSGLVKCDDRLIILLDIEEIAKNLNTVGDKNE"
     gene            complement(4342..6369)
                     /locus_tag="PK733_03315"
     CDS             complement(4342..6369)
                     /locus_tag="PK733_03315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOJ09603.1"
                     /translation="MDIKKYLEMFIEEAGENLQRMNQALLELEQNPDNESTLNNMFRI
                     AHTLKGMAGTMNFKKMTKLTHTMEDVLQEVRNNELMLNPELIDILFKTFDALDIYLKN
                     IIDTGHEGSNDYDEIINMLGTIRKGESKTVEVSNTASNKNIFDKGVENAERAPSVKFN
                     EYEKNVIEKAFDMGMKVYYITVYLDKGCVLKSARAYLIFQLIEKHSDIIKSIPIVEDI
                     EDEKFDDSFSVVAISKEDEELFIKGLYSISEVESVIINEIKKDNTTGIEKAKNQPSKL
                     ETEPEVVCENAVSSTKSKSVKTVRVDIEKLDILLNLVGELIIQKTRLEEIYDDKNQIY
                     LESVQHLERIISNLHDAVMKARMVPVEMVFNRFPRMIRDISKELGKEIVLTMSGEDTE
                     LDRTIIDEIGEPLVHLLRNSIDHGIEMPYERKRLGKPEQGHIYLKAYQEGNNVIIEVE
                     DDGHGIDLEKIKEKAVEKGIVSKSETLNMTKNEVIDILFKPSMSTSEKVGNLSGRGVG
                     LDVVKTKIESLGGVVEIETQLGKGSRFIIRLPLTLAMIHALLVYVGGERYAIPLSNVR
                     QIVKVNLDEIKVVQKREVILLNGIIVPLIRLDKLLNTPESSAHKKGITVVIASKGEKL
                     YGFTVDSLIGQQEIVIKNLGKYFSAIRTISGATILGDGEVALILDINSLAF"
     gene            complement(6421..7104)
                     /locus_tag="PK733_03320"
     CDS             complement(6421..7104)
                     /locus_tag="PK733_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOJ09604.1"
                     /translation="MLLREMKTGTKLQLELYDEMGKRIDKQFVSQFEYTEDEKTAIIH
                     VPLYEGVIYPIHTSLLLYVFFSQAEKLYSFKAEIIDRFTKGDIPFIRIKILGKITNVQ
                     RRSYYRLPCSLPAIYRIASQKEFNCESEHGEFIESNTINISGSGVCILLKDNIQADTL
                     VECYLELKEDYEIYFLGRVVRVTKCQQNNNYRYKAGIAFEHIKGRHRDAIVKFIFEEQ
                     RKLRRKGFI"
     gene            complement(7225..8139)
                     /locus_tag="PK733_03325"
     CDS             complement(7225..8139)
                     /locus_tag="PK733_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOJ09605.1"
                     /translation="MNDQAEKLREIITNLKKKQINSKNYSVSNRFNAKTARTIAFTSG
                     KGGVGKSNIAVNTAIALSKLDYKVALIDADLGLANVDVILGLSLKFSLADIMRNNLNI
                     TEVLSEGPCGIKFISGGSGVEELTKLNGEDLERFINNIGILDNLFDIILIDTGAGLSD
                     SVLSFVMAANEVIIVTTPDPTAIMDAYALIKAIAARDNEKNINIIVNRADSIKEANGV
                     LEKLILVSRKFLGIEISPLGFILYDDAIVRAVKLQQPFLLSSPNSQASRFVRDIALNL
                     VEYRKYIPNLKSSGVKDFIKRLSTYFSG"
     gene            complement(8166..9353)
                     /gene="flhF"
                     /locus_tag="PK733_03330"
     CDS             complement(8166..9353)
                     /gene="flhF"
                     /locus_tag="PK733_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOJ09606.1"
                     /translation="MKIKRYVGNDLQETILKVKMDMGNDALIVSNRNIREKGFLKLFS
                     KPMVEVIAALDEPDNRQKEFQLKNSIDNTLKMPAKADENKLKFSDAGRKIQILESRFS
                     NMEETLQKIYLQMQKLPIDGEANKISNKKQNGNSVMQLFYNNLIKNEVEEEIAKQLVE
                     NAYIELEGKNSVNDFISVLYNNISEIIGSPGTINLKENGKPTVVTFTGPTGVGKTTTI
                     AKIAAIYSLEHSKKVSLITADTYRVAAVEQLKTYADILSIPLTTVYSANDIKKAIKTY
                     SDKDIILIDTAGRSHKDSTHFEELKNLINATEADETYLVLSMTISAGNCRKVLNSYGF
                     LDDYKLIFTKYDESSTPGIILNTAFYTKKHLSYITTGQSVPDDIMVCNLDEIIKKMIG
                     NFS"
     gene            complement(9355..11379)
                     /gene="flhA"
                     /locus_tag="PK733_03335"
     CDS             complement(9355..11379)
                     /gene="flhA"
                     /locus_tag="PK733_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOJ09607.1"
                     /translation="MKLKEISVPVTMVTIILAFILPIPGFILDVLLAVNIMLSAIILL
                     NTIYLKDAEDISIFPSLLVITTMYRLSLNVSTTKLILGEGEAGKVIDGFGRFVARDNP
                     VVGFVVFLIIMLVNFLVITKGSERIAEVAARFTLDAMPGKQMAIDADLNSGLITEAEA
                     RERRKKIQKGADFYGAMDGASKFVKNDAIAGIIITFLNVIGGLIIGMVMRKEQFGEAL
                     QNYAILTIGDGLVSQIPALMISASTSFVVTRAASDSDISEDLVKQIFSNYRVLYIASG
                     LSFVLSFFLAKVPFLILSAILGFIGYTLSKQERKKEFTQEEDAHENEVEEIRRPENVM
                     TLLQVDPIELEFGYGIIPMADVEQGGDLLDRVVMIRRQLALEIGMVVPVIRLRDNIQL
                     GQNEYMIKIKGVEVAKGELVPDHFLAINPGTVEQEIEGIKTNEPTFGLPAMWINEKQM
                     DRAEVLGYTVVDLPSVVATHLTEVIKQYANVLLGRQEVQSLIDNVRQNYPVIVDELVP
                     KLMTIGEIQKVLANLLKEGVSIRDMVTILETLGDYASVTRDTDLLTEYARQSLGRAIS
                     KKYLDGEKSAVITLDPQLEQTIMDSIHKTESGSYLALEPDVSNKVINNLTKQVQKFSQ
                     FGQQPLVLASPIVRLYFKRLTEHVLPGLIVLSYNELDPKMEIQSIGMVSI"
     gene            complement(11391..12497)
                     /gene="flhB"
                     /locus_tag="PK733_03340"
     CDS             complement(11391..12497)
                     /gene="flhB"
                     /locus_tag="PK733_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOJ09608.1"
                     /translation="MYLKNSRLIMNLQLFAEDRDSKTEKATHKKRQEARKKGQVFQSR
                     EITSATVLMFAFLSLKAFGNIMYKEISAITVRTLTQNLDMDGYFNIKGVSLLFLEVMK
                     SLAIITGPILVISATAGFLGAYSQVGFLFTAEPIKLTLDRINPFQGFKRLFSPRGAVE
                     LLKAVFKIGITGYIAYRYLNGETANIISLMELEPKQTGIYIYNTSINAAVKICFALIL
                     FGVADYGYQWWEYEKNLRMTKQEIKEEYKQVEGNPEVKSRIRQKQRQMSMRRMMHEVP
                     EADVVITNPTHYAAAIRYDTDISDAPIVVAKGEGYIALKIKETAREHNVQIVENKELA
                     QTIYKTVDIGEAIPPELYQAVAEILAFVYNVKSS"
     gene            complement(12505..13299)
                     /gene="fliR"
                     /locus_tag="PK733_03345"
     CDS             complement(12505..13299)
                     /gene="fliR"
                     /locus_tag="PK733_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOJ09609.1"
                     /translation="MDLLKILNLSGPEMFLLIFVRMTGFFVIAPLFGRRNVPYYIKIG
                     FSLSMALIIYYSAGDFNIEYGDMLYNYVLKVMFEFLTGLTLGFVSYAMFTSIYVAGQL
                     IDMQIGFGAVNVIDPLSSIQISLTSNFYFILSMVAFFSFKGHHMLIKALFDSYKYIPA
                     GGGSLGYGDNIASNGIEIIGNIFIMGFKIAAPITAAILIADIVLGVISRTVPQFNVFV
                     VGMPLKIALGLIVAVITIPMLMELIKTLFYDMDKEMLNLMKGMAGR"
     gene            complement(13375..13644)
                     /gene="fliQ"
                     /locus_tag="PK733_03350"
     CDS             complement(13375..13644)
                     /gene="fliQ"
                     /locus_tag="PK733_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOJ09610.1"
                     /translation="MDQAIISNIAQQALLTVLYISAPILGLSLLVGLAVSIFQATTQI
                     QEQTLSFVPKIIAVAAAIIIFGPWMLNKLMEFTRGIYLNMNAYIK"
     gene            complement(13695..14486)
                     /gene="fliP"
                     /locus_tag="PK733_03355"
     CDS             complement(13695..14486)
                     /gene="fliP"
                     /locus_tag="PK733_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249122.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOJ09611.1"
                     /translation="MKRNIKNRNYTYCLTISTILIVVIVALPASNVLAEPLLPFRFSF
                     GAEPATNPGEVSTSIQILLLITILALAPSIIIMMTAFTRVVIVLSFMRNALGTQQMPP
                     NQVIIGLALFLTFFIMQPVYTEINERAVQPLERTEIGQQEAINISTSVIRKFMLRQIR
                     DEKDLALFVSLSGVETPVDPEALPITVVVPAFLINELTIAFKMGFLIYIPFLIIDMVV
                     SSTLMSMGMMMLPPVMISLPFKILLFILVGGWNLVARSILESFVR"
     gene            complement(14509..14868)
                     /locus_tag="PK733_03360"
     CDS             complement(14509..14868)
                     /locus_tag="PK733_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOJ09612.1"
                     /translation="MEKFIYVLGLMVVFASILFLAYISTKYIGSKAGQIGRGKYLRVI
                     ETICLGKDRYLHLIKAGEEFILVSSSVKGIEVLKSTDEINMDEISENADGSYSNNIKV
                     NLNRIGALFQGFEYKKK"
     gene            complement(14923..15285)
                     /locus_tag="PK733_03365"
     CDS             complement(14923..15285)
                     /locus_tag="PK733_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09613.1"
                     /translation="MGKRILVVDDAAFMRMMIKDILTKAGFEIAGEAENGLRAVEKYK
                     DLAPDLVIMDITMPEMDGIQAVKEIRKIDSNALIIMCSAMGQQAMVIESIQAGAKDFI
                     VKPFQADRVVEAVKRALG"
     gene            complement(15303..16460)
                     /gene="fliY"
                     /locus_tag="PK733_03370"
     CDS             complement(15303..16460)
                     /gene="fliY"
                     /locus_tag="PK733_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HOJ09614.1"
                     /translation="MEHALSQSEIDALLKGIPVSDINNIQNTEDNEELSLEEIDALGE
                     IGNISIGTSATTLFTLLGQKITITTPKVTLTTWEQLAKEHNLPYVAIRVKYTAGLEGA
                     NLLILKEDDVKIITDLMMGGTGRITDGELTEMHLSAISEAMNQMIGSSATSMSTMFNK
                     RIDISPPTAFIIKFDSLNHYDDFYSPENGKIVKVAFKMVVGDLIDSEIMQLLPLPFAK
                     TLLNNLLYKEPETVKESKDTGSNIIRKTESYNEIKNEEKNQDKYQPKNDLYESFKQND
                     SSYKDKGAVNVQPVHFQSFEDETVTDEKQNIDIVMDIPLQVTVELGRTQKLIREILEF
                     NYGTIVELDKLAGDPVDILVNGKRVAKGEVVVIDDSFGVRITDIVHPSKRF"
     gene            complement(16463..17449)
                     /gene="fliM"
                     /locus_tag="PK733_03375"
     CDS             complement(16463..17449)
                     /gene="fliM"
                     /locus_tag="PK733_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOJ09615.1"
                     /translation="MGDILSQNEIDELLKALGTGELNVKDIKDASGEKKIINYNFKRP
                     SKFSKDHLKALKSIYENYARLATNFLTGYLRALVQIDVIESESLTYSEFIGSISNPLV
                     LAVVDFTPLEGSIIIEIPPKIVFAFIDRILGGKCNTFEDEREFTEIELAIIERIVVQM
                     LNVMREPWQNIIQIRPILEKIETNTQFARIISPNEVVALVTLSIKLSDVEGMINICIP
                     YITVEPIIEKLSTRYWFTSIERQTDSSSKELIESKISATKVPLKAIFGSTSITVSDFI
                     ELQKGDVLPLDNDINSELDVYVGNLLKFRGKPGVKKNRVSIKITALVGKEDE"
     gene            complement(17468..17977)
                     /locus_tag="PK733_03380"
     CDS             complement(17468..17977)
                     /locus_tag="PK733_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOJ09616.1"
                     /translation="MGNKGSFIILLVIIAILTMAVVMLVGYIYVLGAKPNEKQTMYEN
                     NELIRRPSDEELGIKKLFDEKQFFVLKNENIARQSAIRVGISLRYFKRVKGIKNCEKK
                     INDFESEIRELVGTYFQNVTIDQVKNLDFKIRAKDDLKNQINDLLNSSEKIQNDIIYD
                     VVFDDWFYQ"
     gene            complement(18027..19352)
                     /locus_tag="PK733_03385"
     CDS             complement(18027..19352)
                     /locus_tag="PK733_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOJ09617.1"
                     /translation="MMRSMFAGISGLKAHQMKMDVIGNNIANVNTIGYRAGRITFQEV
                     FNQTLKGASAPDSALGRGGTNPMQIGLGTGIGSIDTIITGGSQQRTDSPLDLSIDGDG
                     FFIVKGSKADTFMFTRAGNFTRDLLGNLVTSGGMSVYGWMDYGEKMNDDGTFSFNVNK
                     PVEPINLYTDDYNGNKQVIAAKATENISFAGNLDTRYPTVAAGDDAQIIVPMIVYDSL
                     GNEYEIAMAYRKIEAAGGITTWEWSIKGGDLTTTASGEISFDSDGKIILDAPDATNPR
                     VTPVINLIPADSIGSKAFDVKLDLRKLTMFADESSVKPLNSDGYPPGKLSSFSIGNDG
                     IIMGIYSNGKPKPLGMIALAEFENPAGLQKTGNNMYIQTSNSGVFRSGIKPGTGSTGT
                     LNPGTLEMSNVDLASQFTDMIITQRGFQANSRIMTTVDEMLQEMVNMKR"
     gene            complement(19357..19803)
                     /locus_tag="PK733_03390"
     CDS             complement(19357..19803)
                     /locus_tag="PK733_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOJ09618.1"
                     /translation="MQVNSVVNIEDTTEIVKKHSNSNGNRNTNELGKDEFLRLLVTQL
                     KYQDPLNPVDDKEFIAQMAQFTTLEQMYNLNSSFSSMKALNLIGKSVKAEIIDDRTGE
                     IKSISGEVEGVKIDGRESYAVVDGIDIPIESIKMVETAEKITNEEV"
     gene            complement(19826..21346)
                     /locus_tag="PK733_03395"
     CDS             complement(19826..21346)
                     /locus_tag="PK733_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOJ09619.1"
                     /translation="MTNGISAGLEVRSAQSPTPVANKFILLQGLSFSGIFKQKDTSFQ
                     SGGEKQELGSNKKYAPLIKKSNLLVDNKTKRSIKANQENDKKDNEIKGKQEKKTNINN
                     EYTFILHTFQHKEYIGEKFEDITHKEEIVKIWLESSEIGNDPLMVLQRLLQIYSGDLV
                     EVHEGQSDAVNEFNMLEPDFRELINFLYEDDEYRDILTDILDKKDIVNDIETDNIKQL
                     LEKMKIEFESRSGKVAAVQSENDTEKHVAEEDLVNDSEIINASSGVKYVEENNIPGVE
                     LNDDDNNQNLNMLNVKLSSESYLADAGNKELSSPETDLQSKGASIKKEDEYTDGDFTR
                     VLFPEGFSKEASSIMLEQPVSHNQNKTNEIISQIVDKARILIAGKKYEMLIEMKPEIL
                     GKVTLKVITENDAVIARFITENQHVKEVLESNFQTLKDSLKEQGLTVQGFSVSIAGDD
                     SDKSYNNNQRNWNLMPENKNRRIQTRVEITDNIGISNEDTGYNQWNWSGNRINLMA"
     gene            complement(21351..22100)
                     /locus_tag="PK733_03400"
     CDS             complement(21351..22100)
                     /locus_tag="PK733_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
                     /protein_id="HOJ09620.1"
                     /translation="MSKNVLFTLLVILTALVIMCIIIGGAFYMVIHNNVYGLGEKYRE
                     QIQNITLLKWALPKPMDSDDPKYLNDKEIREKYSEIKIERDDLLKQLNEANLEINRLI
                     IYKQAYEKSIQDKEKEKDYLAEEMKKLEKSKEEIQLLAVDGNKEGFKKYFETIDKEKA
                     EKIYAQIMQDEKTEQDIKKFLQIYENMDEAAAARIFIEMGEKDIKLVVDILSGIKKEK
                     ASGILAEMKPEFASKVTREMSKVMLKTGEGR"
     gene            complement(22100..22552)
                     /gene="fliJ"
                     /locus_tag="PK733_03405"
     CDS             complement(22100..22552)
                     /gene="fliJ"
                     /locus_tag="PK733_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOJ09621.1"
                     /translation="MKFNFRLKSVLNIKEQAEDILKIELGEALGKLDMENKKLDALTR
                     ESSRYMQDYRMLLYVGAKTTDMNSFNIYFGKQKEKIEMQKESINKAKENVDKIREELI
                     KVSQEKEMLEKLKNKKQILFNKELLKEEQKFNNEIISYRQNMKLVEDM"
     gene            complement(22555..23868)
                     /gene="fliI"
                     /locus_tag="PK733_03410"
     CDS             complement(22555..23868)
                     /gene="fliI"
                     /locus_tag="PK733_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOJ09622.1"
                     /translation="MSPINLGKYKDILDKIPLIEYSGSVTRVIGLTIESKGPEANIGE
                     LCRIHTMKYDREIYAEVVGFKDNNVLLMPFGDMTGIGPGNLVISEGRTLNVKVGEELI
                     GRVLDGLGTPIDGKGYIDTKERYTVDNQPPNPLMRKRIKEPMSLGIKAIDGLLTVGKG
                     QRIGIFAGSGVGKSTLIGMIARNTMADINVIALIGERGREVREFIEKDLGEEGLKRSV
                     VVVATSDQPALIRLKGAQVATSIAEYFRDQGKDVLLMMDSLTRFAMAQREIGLAIGEP
                     PVSKGYTPSVFGVLPKLLERAGTSDKGSITGLYSVLVDGDDLTEPVTDTARGILDGHI
                     VLSRELANKNQYPAIDVLASVSRVMPDVVSGEHKKISGRIKKVMAIYKDAEDLINIGA
                     YVKGSNNNIDFAIDHIESILNFIQQDTNTNYTFDDVLNMLEKVFD"
     gene            complement(23869..24705)
                     /locus_tag="PK733_03415"
     CDS             complement(23869..24705)
                     /locus_tag="PK733_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOJ09623.1"
                     /translation="MKLLSSKVIKNCKVCIGKPFVVEIPEEELCVNEKKYIKESRIKE
                     LDKGYISTPFIIEGEKGYAVNKFRTKEIIENAQRQADLIIKEAKSEYQRVIDDSRIEK
                     EKCSKAIEEARKKGYSEGYNEGFDIGRSEAAEKFEKINAELDLIKKQAREDYRQMLAE
                     AEPDAVGVILDIAEKVLQHEITYNKDIILGLTKEAFDKCSEKKELILRVSPDDYEYLI
                     ENSKSFDMLFGEAVDFDIRKDPSLKAGGCIIDSPQGSINTSIEMKLDKIKKVFNDIQE
                     VL"
     gene            complement(24689..25705)
                     /gene="fliG"
                     /locus_tag="PK733_03420"
     CDS             complement(24689..25705)
                     /gene="fliG"
                     /locus_tag="PK733_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOJ09624.1"
                     /translation="MVRSIQRERSGKEKAAMLLISLGPERSSEIFKHLREDEIEQLTL
                     EIANIRFVSPEEKEKVLKEFYQICLAQEYISEGGIEYAKKILEKAMGSQKTLEIINRL
                     TASLQVRPFDFVRKADPEQLMNFIQKEHPQTVALILSYLKPQQAAAILSALPQDKQAD
                     VASRIALMDRTSPEIIKEVERILEKNLSSIAIDDFTSSGGVQTIVDILNNVDRSTEKY
                     IMETLEIGDADLAEEIKKRMFVFEDILTLDNRSIQRFLKEIDNNQLVVALKGAGEDVH
                     KIIFANMSKRMSEMIKEDMEFMGPVRLRDVEEAQQKIVNVIRRLEEAGEIVISRGGGD
                     EIVV"
     gene            complement(25717..27315)
                     /locus_tag="PK733_03425"
     CDS             complement(25717..27315)
                     /locus_tag="PK733_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HOJ09625.1"
                     /translation="MPDMISRLIKQIKEFWAGLEKSHKTRIYITSSIVIVAVTIGITT
                     LTKPSYTTVVSGAGKKEIGEMSSILTENKLWNDVGKDGESLIVNSKDNGRAQILLAEK
                     GYPKGGMTFEDAIKMIGITTTENDKKHIWKQQQANEIAKKIMAHDNIEYAEVSLALPE
                     QSVFLTSDKKEYKPTAFIMVKPKNYITQEQVQGIVMLVSKSVERLDPKDITVVDNNLN
                     LLNSYAPDNEITVLTSQEEMRTKKAKELEAKVYSYFSVGQFDNFDTLRVVVNPFLDFD
                     KVKTQSKNITNPSDMDGGAVISSEERTEKTENVSRGDIPGTDTNPGEAGSPTYLLGND
                     GNSTYSLKEGKQNFGYDEALKEEEKALGYLIPDKSSMAISLWYGRSVIDGNGISDEFI
                     DQIRIAASMATGIPENNISVSKYKLAAQDSAQIQTGDLKEIIVKDYLWLIILGLLIIG
                     LTITVISMKKHNKKDESAAIENDETKSEYMVPEKIEEYLKDINEVKLDEKSKIQKKID
                     DFAKDKPEAIAQLIRNWLSDTRDS"
     gene            complement(27352..27651)
                     /gene="fliE"
                     /locus_tag="PK733_03430"
     CDS             complement(27352..27651)
                     /gene="fliE"
                     /locus_tag="PK733_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOJ09626.1"
                     /translation="MDIGAIDKSLSISSLKKTGIQRTEDGIKGFADYFKEAVNKTNDI
                     IVKAENISNQFAVGNIDDIHKVLVEVEKADIALQFTLQIRNKIMDAYNEIMRMQI"
     gene            complement(27739..28188)
                     /gene="flgC"
                     /locus_tag="PK733_03435"
     CDS             complement(27739..28188)
                     /gene="flgC"
                     /locus_tag="PK733_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOJ09627.1"
                     /translation="MGFLDSLGAATSGLTAQRLRMDIISQNISNINTTRTSNGMPYRR
                     KVVLFGERNNNMPFSHFLSESSRNKFLKTENGIRVTHIAEDTSPFKRIYDPGHPDADQ
                     DGYVLMPNVDIITEMINMISASRAYEANVTSINATKSMALKALEIGK"
     gene            complement(28192..28620)
                     /gene="flgB"
                     /locus_tag="PK733_03440"
     CDS             complement(28192..28620)
                     /gene="flgB"
                     /locus_tag="PK733_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOJ09628.1"
                     /translation="MVNKIFSNIKFTEKALDAAWLRNKAISQNIANIDTPGYKRKTVI
                     FEELLKNALDNNSEGLKTDSGHLEKGKDKVDNIDNIDNMQIRMLTDNKNLSTRLDGNN
                     VDIDNESASMAKNTIKYNVLIQSINSSLKMLKSVISEGRR"
     gene            complement(28878..30989)
                     /gene="topA"
                     /locus_tag="PK733_03445"
     CDS             complement(28878..30989)
                     /gene="topA"
                     /locus_tag="PK733_03445"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOJ09629.1"
                     /translation="MAYKLIIVESPGKVKSIGKFLGKNYHVMASAGHVKDLPKSHMGI
                     DIENNFEPKYITIRGKGELISKLKKEAKGAERVYLATDPDREGEAISWHLANLLDVDE
                     VSKCRITFNEITKNAVTNSLKVPREIDMKLVNAQQARRVLDRIVGYKISPILWKKVRK
                     GLSAGRVQSVAVRLICDREEEIEKFIPEEYWTITANLTKKNSKTLFKARFYGVGNKKV
                     DLKNKSEVEKILDKISGQDFYVNKVKKGERKKSPAPPFITSTLQQEASRKLGFTTKKT
                     MMLAQQLYEGVEIKGEGSVGLITYMRTDSTRISNEALDEVREFIDSKYGKEYTPEKSR
                     VFKNKSSSQDAHEAIRPSNISLLPDSVKESLSRDQYRLYKLIWERFVACQMSSAVYDT
                     LNVDILAADCVFKASGSRIKFPGYTMVYREDNDDKDQKNAEQGEDNSNLPELVEGEML
                     SLKKIEHEQNFTQPPQRYTEASLVKALEEKGIGRPSTYAPTISTIISRDYVVREGKNL
                     LPTELGRIVTGIMKNHFENIVNVQFTAQMESQLDDVEEGEKDWVDILKDFYADFHKTI
                     GQAELEIGNVEIPDEETDVKCEKCGKNMVIKTGRYGKFLACPGFPECRNTKPIVEEAG
                     VLCPKCQAKVLVKKTRKGKKYYGCENNPKCDFMTWDKPAGENCPECGSFLVIKYSGSK
                     SQIKCSNEKCSYSRDKKKENE"
     gene            complement(31144..>32124)
                     /gene="dprA"
                     /locus_tag="PK733_03450"
     CDS             complement(31144..>32124)
                     /gene="dprA"
                     /locus_tag="PK733_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ09630.1"
                     /translation="ELRILPFLTEALIQQLIDVKHKNNVCKHLEAIRKNQIEVITIHD
                     ELYPYYLKNIYDPPVVLYCKGKILANENAISLVGSRKATAYGLEAGEELAYQLACLGI
                     TVVSGMARGIDSSAHKGALKAQGRTIAVLGCGLDVVYPAENKVLMNNILEKGAVISEY
                     LPGTMPFQRNFPARNRIISGMSLGVVVVEAAADSGSLITANFALEQGREVFALPGNIN
                     RINSTGTNMLIKEGAKLVMSVKDILEELSIYNLEGENKIKNPIEQTRYKGLDKEERRI
                     VECLKSESMYVDLLAKKAGYDIKYTNSILTMLELKGIIEQLPGKIYKLKI"
BASE COUNT         9217 a         6802 c         4367 g        11738 t
ORIGIN      
        1 cgatttgaaa aatgattctg gggtaagaga tattgtcatc acaggaagag ccgattttta
       61 atgcaaggtg tcaaacgcag tgtcaaggta atttatactc tcctattcgc taaaaaacag
      121 gctttcaaga aaatcttgaa agccttggtg ccgaaggtcg gactcgaacc gacatggtat
      181 cgctaccgct ggattttgag tccagtgcgt ctgccaattt caccacttcg gcatattgat
      241 tatcactgac gcaaaaacca ttatacatca gcaactaaac aatgtcaata ctttttgcgc
      301 agatttaaaa cgtattttta ttccatttta ttccatatgt tctcctatat tcttacatca
      361 taagctgcaa tacaagatgt acttctcctc tacctatatt aagagtacgg gcaatctctg
      421 cttcattcat ccccttctcc aaaagtaaaa ttacgtcttc atatttttta tttactgtga
      481 aattttttat tgcgccttgc tttatttttc ccttactggt tgcatcaatt ccactattag
      541 tatcatttat atcttttatc ttattatttt tacaactttc cacaatatct atcgcataca
      601 tactattatc aatatcttga cgggaatcct ccccgtattt ttcgactaaa tccaactttt
      661 ccgacaactc ccctgccttt tcctcaatga aattaatctg agttgagata ttgttaattt
      721 ttatatccaa ctcgtttttt ttctcttcaa tccggtctat tataaaatca gacaatttat
      781 ttaattcatc taccatgtac tccgcgtcca taacaatttc acttaattgt ttctccttct
      841 cttccatctg tttcatatca ttaataaatt tttttctgtc ctctagaatc agaaaagtac
      901 atactattaa cattattaat ccaactatca aaaatattgt ataaaatggg ctcaattcaa
      961 tcacctcttt ttctactatt atacaaatgg actaactttt atatcgacgc catcgcaaaa
     1021 caatgtacaa tacttcagtt cttctttaat atacattgtt gatgatccaa ttgtaacttt
     1081 tacaccaggg tgtatggacc ccaggcatcg caatttcccg gcagattgca tgctaatcat
     1141 tttttcaatc tgctctatat cataatttag ctgagataat tttgatttat aaaaatctct
     1201 tgtaaagaga cttttttcat atataccttt cttatcctca gttaacatat tattactttc
     1261 aagtttggac aatatggaaa taacttgttc ggttttttgc aagctatttt gaatggaatt
     1321 tatttcactt tttatattca tgacagcatc ccgaagatca gagttcacac caatttctat
     1381 ttcagtaagt gttgccattt gtgaaccgat aacttttgca tatagttctt tacataccgc
     1441 actactgcct ccaaccagaa gtccaatcct tccgccaagt tcgagacttc ccccacattt
     1501 tactgaactg tgcattattg tttcagcttt aatatcattc atagcttcaa ctttactttg
     1561 ctcaataaac ttagcaacta tatcaccatt actttttatg tatgccttat tattaccctg
     1621 cactcctctt ctaataacaa tatttccttc tgatattact tccgctcctt caacaatacc
     1681 ccatatttca atattaccac ctgcaacaac agaaaatcct gacaaaacat tgccccggac
     1741 aatcacattg ccaacaaagt tgatattacc agtagaattg tccacatcac cctttatttc
     1801 ataacttgtg tacacactga ttttcccatt ggaatagctt acctgaccat ctactgatgc
     1861 aattaacgaa ttactgtcat ttaatatctc tacatttttg cctacaggca atctagcagt
     1921 tttcccttca tatgcgggta tttctgaatt cagtacatcc ctgccgccta ttccttgagt
     1981 agggggaact aaagcacata aaacctggcc tttacgcaca ctctctatta atcctaagtc
     2041 tctgaaatca actttgccgt tttctaatat tatgggttta taatttttat ttaagtcaaa
     2101 ttggaattgg acctgtccat ctttaccatt tacaggtgat aaaccttttg cagcaattat
     2161 ctttttgtaa aatatatttt gcttgacagc tagctcaatt atctcccgat caaccccgaa
     2221 agttacgcca tatgacttta gagcttccag aataccttct acagtatggc tttgaccgta
     2281 ctcgggcgca aataatgtaa tgaaagcact catcttatca gaagaaacgt caaccaatac
     2341 taatgtatct ttatttatac cttcttcatt agcttttatg ttattgtttt cattatttat
     2401 atctgccaat tttatcacct tcttttatag ataacgcata aaagaaacta cattacaaaa
     2461 gttttatgcg cttatgttac cttagcgaat acgtaaaaaa acaatttgtt ccttgtggaa
     2521 tcatttttgc gttataaata ggttggtttc tagcttttaa tatacccggc aagcttgcct
     2581 ttaagcctta aaatagcttt agtatggagc tgagatattc tggattctga tactttcagt
     2641 attgcaccta tttctttcaa tgtcaactct tcaaaatagt ataaagatat aacaagcttt
     2701 tctttttcag gcagtttttc aattgactcc cctaaatagt cttttaactc ggatatttca
     2761 aaacaatact ccggcatatc atcgccatta atatgcaaca tttcactatc aatctgaaaa
     2821 ttctgctcta gaaactcatc aagagatact aatgatacca aattcacttc atttagaagc
     2881 ttattatatt cctctacagt tattcccatg ttttcagcaa cctcataatc tgttggtggt
     2941 ctaccctctt tagtttccag ttcacagtat attctttcta ggtccttgtt tttttgtctt
     3001 agagatctgg gtacccaatc taattctcta atactatcta ttatcgcccc tcgtatacgt
     3061 aatgaagcgt aggtttcaaa ttttacgccc ttgctaatat caaatttatc aatagcatct
     3121 atcaagccaa atacaccaaa gctaacaagg tcatcatatt caatattgga accaaaatag
     3181 atattcatcc tgccagctat atatttcaca agatataaat atttaattat gattttgttt
     3241 ctaattaata tgtctttcgt atctgcatat tgtttccata tattgcagtt atattccaat
     3301 gatgacatta ttcatcctcc gctctagatc cactttgttc caatcccaag ggttttcacc
     3361 ataagaccgc cattctctga ataaaactcg atagtgcgta ccaggtttcc ccctgtatct
     3421 tcagcaacaa ccagaattcc aagctcactg agtttctctt tggctgcaat tacatttctt
     3481 tcaccaattc tcatcaattc cgatgtttca ttaaaattaa acatctgtgc acctccggca
     3541 agttttgctg tgatattttc tattgatgca ccaagccggt tcatatcgtg cagcatctta
     3601 ttaattgcag tatcggcata tttggccgca ttaatattac tactggaatt gggactcaat
     3661 ggcaacatta tatgagctaa tccaatagct ttagttttca catcatacat tgcaattccc
     3721 acacatgagc caagcccttg tgtaacaagg attccaggat gctttgatgt gtttaattcc
     3781 gccataccta tatttataat gttactcatt tttatctcca actgtattta agttttttgc
     3841 aatttcttct atatccagta aaatgatcaa cctatcatca catttaacaa gacctgaaaa
     3901 aaaatcagaa ctatcttcac cagatatacc tgacactttc tcaatagtgc ttttattgat
     3961 ttcaattact ttatttacct gatcaacttt aatacctaca ctatactcct ccatatttac
     4021 aataataatt ttagtctcct gagtatcttc gaccccgggg aggttaattc tggttcttaa
     4081 atccattaca ggtacaatat ctcctcttag attaattaca cctttcatgc aatcagatgt
     4141 ccccggaact ctagtaattg agtaattgtt ttctacaatt gtactaataa aacttatatc
     4201 aattccatat tcttgtgtgc caagcgtaaa agataataac ttaaaactat ttactgctac
     4261 tgcctcttcc atttgaaaag actcctttct atttaaaaat taattaaaaa ttatacaaaa
     4321 ttatataacg atactattta attaaaaagc taaagagttt atatccaaaa tcaaagcaac
     4381 ttctccatct ccaagtattg ttgctcctga aattgtcctg atagcggaaa aatattttcc
     4441 taaattttta attacaattt cctgttgacc tataaggctg tccactgtaa aaccatataa
     4501 tttttcacct ttagatgcaa taactacagt aatacctttt ttatgtgctg atgactcagg
     4561 agtatttaat agtttatcaa gccttattaa gggtactatt atgccattca gtaatataac
     4621 ttcacgtttc tgaacgactt taatttcatc taaattaacc tttactattt gtctgacatt
     4681 acttaaaggt atagcatatc tttctccacc tacatagaca agtaacgcat ggatcatagc
     4741 aagagttaaa ggcagacgta ttataaatct gcttcctttg cccaactgag tttctatctc
     4801 aactacacca cccaatgact caattttagt ttttacaacg tccagcccta cacctcttcc
     4861 agacaaattg ccaacctttt cactagtact catactaggc ttaaataaaa tatctataac
     4921 ttcattttta gtcatattaa gtgtttcact tttagaaact attcctttct ccactgcctt
     4981 ttctttaatt ttttccaggt caataccatg tccatcatct tctacttcaa ttattacatt
     5041 atttccttct tggtaagctt tcagataaat atgtccctgt tcaggtttac ctaatctttt
     5101 acgttcataa ggcatttcaa ttccatggtc aatagaatta cgcaacaagt gtacaagagg
     5161 ttctccaatc tcatctataa ttgttctgtc taactccgta tcctcacctg acattgttaa
     5221 tacgatttct ttacccaatt cttttgatat atccctgatc attcttggaa atctgttaaa
     5281 aaccatttct acaggtacca ttcttgcttt cataacagca tcatgcagat tactgataat
     5341 cctttcaaga tgctgcacag attccaggta aatttgattt ttgtcatcat atatttcttc
     5401 aagccttgtc ttttgaataa tcagttcgcc cacaagatta agcagaatat ccagcttttc
     5461 tatatctacc cttacagttt taaccgactt ggattttgtt gaagatacag cattttcgca
     5521 tactacctca ggttcagttt ccaatttgga gggctggttt tttgcctttt ctatccctgt
     5581 cgtattatct ttttttattt catttataat aacactctct acttcagata tagagtagag
     5641 ccctttgata aacaactcct catcttcttt tgaaatagcc acaacagaaa atgaatcatc
     5701 aaatttttcg tcctcaatat cttcaactat cggtatggat tttattatat cagaatgttt
     5761 ttctataagc tggaatatca aataagctct tgctgatttt aatacacatc ctttatcaag
     5821 ataaacagtt atgtagtaga ccttcattcc catatcaaaa gctttttcaa taacattttt
     5881 ttcatattca ttaaatttta cagaaggcgc tcgttctgca ttttctactc ccttatcaaa
     5941 aatattctta tttgaagctg tattgcttac ttcaactgtt tttgattctc ctttcctgat
     6001 tgtcccaagc atatttatga tttcatcata gtcattactg ccctcatgcc cggtatcgat
     6061 aatatttttc aaataaatat caagagcatc aaaagtcttg aataaaatgt caattagttc
     6121 tggattaagc atcaactcat tatttcttac ttcctgcaga acatcttcca ttgtatgagt
     6181 cagtttagtc atcttcttaa aattcatggt ccctgccatg cccttaagtg tatgagctat
     6241 cctgaacata ttgtttaaag tagactcatt gtccggattc tgttccaatt ctaataatgc
     6301 ttggttcatc ctctgcagat tttctcctgc ttcttcgata aacatctcta aatacttttt
     6361 tatatccatt gaacctcacc cctaaataca tttattctgt aagatttccg ttgcagccgt
     6421 ctatataaag cctttccttc taagctttct ttgttcttca aaaataaatt ttactatagc
     6481 atccctatgt ctgcctttaa tgtgttcaaa agcaattccg gctttatatc tatagttatt
     6541 gttctgttga cattttgtaa ccctaacaac cctgccgaga aaatatattt cataatcttc
     6601 cttcagctct aaatagcatt ccactaaagt atcagcttga atattatctt tcaggagaat
     6661 acaaactcca ctgccactaa tattaatggt attgctctct ataaattccc catgctcact
     6721 ttcgcaatta aattcttttt gggaagctat tctgtatata gccggcaggc tgcatggtaa
     6781 acggtaatag cttcgtcttt gaacatttgt aattttacct aatattttta ttctaataaa
     6841 aggtatatcc cccttggtaa acctatcaat aatttccgcc ttaaagctat atagcttttc
     6901 agcttgagaa aaaaatacat atagaagtaa gctggtatgt attggatata taacgccttc
     6961 atacagtggt acatgaataa ttgcagtttt ttcatcttca gtgtactcaa actgactgac
     7021 aaattgctta tcaattcttt ttcccatctc atcatacaac tcaagctgaa gttttgttcc
     7081 tgttttcatt tctcttaata gcatcccctt aaaaaacctc ctgcaataat cttttttaat
     7141 gaaaaatcct cttgcagcta aaatattatc tatagacatg attctaacaa ttaaaagaaa
     7201 tattgtctac aaagcaaaat acttctaacc actgaaataa gttgataaac gctttataaa
     7261 atcttttact ccgctgcttt ttaagttagg tatgtatttt ctgtattcaa ccagattcag
     7321 cgcaatatcc cttacgaacc ttgaggcctg actattgggg ctgcttaata aaaacggctg
     7381 ctgcagtttt accgctctta ctatggcatc atcatataaa ataaatccca aaggagaaat
     7441 ctcaatgcca agaaattttc tcgaaaccaa aatgagtttt tcaagtacac catttgcctc
     7501 cttaatacta tcagctctgt taacgattat atttatattt ttctcattat ctcttgcggc
     7561 aatagcttta atcaaggcat aggcatccat aatagctgta gggtcagggg ttgtaactat
     7621 tattacttcg tttgctgcca tcacaaaact taaaacgctg tcagataatc cagcgcctgt
     7681 atctattaaa attatatcga ataggttatc cagaatccca atattattta tgaacctctc
     7741 taaatcttct ccattaagtt ttgtaagttc ttcaacccct gagcctcctg aaataaattt
     7801 tataccacat ggtccttctg ataacacctc cgtaatattc aaattattgc gcattatatc
     7861 agcaagagaa aacttgagag aaaggcctaa aatgacatct acatttgcca gtccaaggtc
     7921 tgcatcaatt aaggctactt tatagtcaag cttacttaaa gcaatagctg tatttacagc
     7981 tatattggat ttcccaacac cgcctttacc actggtaaaa gctattgttc tggctgtctt
     8041 tgcgttaaac ctgttactaa ctgagtaatt tttgctatta atctgtttct tctttaaatt
     8101 agtaattatt tctcttagtt tttctgcctg atcattcatt ttttactcct ttaacagcta
     8161 attttttagc tgaaatttcc aatcattttc tttattattt catccaggtt gcataccata
     8221 atatcatcgg gtacgctctg accggtagtt atgtacgaaa gatgcttttt tgtataaaac
     8281 gctgtattga gtataatccc aggcgttgat gattcatcat atttggtaaa tatcagttta
     8341 taatcgtcga gaaagccata actgtttaaa actttcctgc agttgccggc acttatagtc
     8401 atactaagta ccaaataagt ctcatctgct tctgtggcat ttatgagatt tttaagttct
     8461 tcaaaatgtg tagaatcctt atgacttctt cctgcagtat ctataaggat tatgtcctta
     8521 tcactgtaag tttttattgc tttctttata tcatttgctg aatagacagt agttaatggt
     8581 atagaaagta tatcagcata ggtctttaat tgttctactg ctgccactct atatgtatca
     8641 gctgttatca gtgatacctt tttgctgtgt tccaaggagt aaatagcagc tatttttgct
     8701 attgttgtag ttttcccaac ccctgtaggt ccagtaaatg taacaacagt gggcttccca
     8761 ttctctttta aatttattgt gcccggactt ccaattattt cagaaatatt attataaagg
     8821 actgatataa aatcgttaac actgtttttc ccttccaact caatataagc attttcaacc
     8881 aactgcttgg caatttcttc ctctacttca ttttttatga ggttattata aaacagctgc
     8941 ataacgctgt ttccattctg ttttttattt gaaattttat ttgcttcccc atcaatcggc
     9001 aatttttgca tttgcaaata tattttttga agtgtttctt ccatattgct gaatcttgat
     9061 tcaagaattt gaatttttct ccctgcatca gaaaatttca acttattttc atcagcttta
     9121 gcaggcatct ttagtgtatt atctattgaa ttttttaact gaaattcttt ttgccggttg
     9181 tcaggttcat caagagcggc aataacttct accataggtt ttgaaaacag ctttagaaag
     9241 cctttttccc ttatattcct attgctcaca ataagtgcat catttcccat atccatttta
     9301 acttttagaa tagtttcctg taaatcattg cccacatatc gtttgatttt cattttagat
     9361 actcaccatc ccgatcgact gaatttccat tttcggatcc aattcattat aagatagaac
     9421 tattaaaccc ggcaaaacat gctctgtaag ccttttaaaa tacaacctga ctatagggga
     9481 tgccaatact aagggctgtt gcccaaactg ggaaaacttt tgaacctgtt ttgtaagatt
     9541 gtttattact ttgtttgata catccggctc cagagccaaa tatgacccac tctctgtttt
     9601 atgtattgaa tccattattg tttgttcaag ctgagggtcc aatgttatta cagcagattt
     9661 ttcaccatca agatactttt tggaaattgc cctgccaaga gactgtcttg catactcggt
     9721 caacaaatct gtatctcttg ttacagaagc ataatcaccc aatgtttcca aaatcgtaac
     9781 catatccctt atagatactc cttctttcaa taaatttgca agtacttttt gaatttcccc
     9841 tatagtcata agctttggta caagttcatc tactataacc gggtaattct gtcttacatt
     9901 atcgataaga gactgaacct cctgtcttcc aagcagaaca ttagcatact gttttattac
     9961 ttccgttaag tgagtagcaa caaccgatgg aaggtctaca acagtataac caagcacttc
    10021 ggctctatcc atctgtttct cattgatcca catagcaggc aaaccaaaag taggttcatt
    10081 ggtctttata ccttctattt cctgttcgac tgtaccaggg tttatcgcaa ggaagtgatc
    10141 cggaactaat tctcctttcg caacttctac accttttatt ttaatcatat attcgttttg
    10201 tcctaactgg atattatccc ttagtcgtat aacaggtaca accataccaa tttcaagagc
    10261 aagctgtctt cttatcataa ctactctgtc taaaaggtcc cctccctgtt caacatcggc
    10321 catgggtata attccatatc caaattccaa ttcaattggg tcaacctgta aaagagtcat
    10381 tacattttca ggtcttctaa tttcttctac ttcattttcg tgtgcatctt cctcctgagt
    10441 aaattctttc tttctttcct gctttgataa agtgtaacca ataaacccta atattgccga
    10501 aagaataaga aaaggtacct ttgcaaggaa aaaagacaac acaaaactta aacctgaagc
    10561 aatataaaga actctgtaat tgctgaaaat ctgtttaacc aaatcctcac tgatatctga
    10621 atcagatgct gctcttgtga ctacaaaact tgtagaagct gaaatcataa gtgccgggat
    10681 ctgactcaca agtccatcac ctatagttag tattgcatag ttttgcaatg cttctccaaa
    10741 ttgttctttc ctcataacca tgccaataat caggccgcca attacattaa gaaaggtaat
    10801 aattataccc gcaatagcat cattcttcac aaatttgctg gcaccatcca tggcaccata
    10861 gaaatccgct cccttttgaa tttttttccg cctttccctt gcctctgctt cagtaattaa
    10921 accactgttt aaatctgcat ctattgccat ttgtttcccc ggcatggcat ctagagtaaa
    10981 ccttgcagca acttctgcaa ttctttctga accttttgtt attaccagaa agttaaccag
    11041 cataataata aggaatacca caaatccaac aacagggtta tcccttgcca caaatcttcc
    11101 aaaaccatca ataaccttgc ctgcttctcc ttctccaaga ataagcttgg ttgtagatac
    11161 attgagagat aatctgtaca tggttgtaat taccaggagt gacggaaata tagatatatc
    11221 ttctgcatct tttaagtata tagtgtttaa aagtattatg gcagacagca ttatattgac
    11281 agcaagaagc acatcaagta taaaacctgg aattggcaga atgaatgcaa gtattatagt
    11341 taccatcgta accggtaccg atatttcttt aagcttcatc catatccccc ctacgaactt
    11401 tttacattat atacaaaagc aaggatttca gcgaccgcct ggtaaagctc aggtggtatt
    11461 gcctccccta tatcaacagt tttataaata gtctgtgcaa gttccttgtt ctctacaatt
    11521 tgtacattat gctctctagc tgtttctttt atttttagag ctatatatcc ttcacccttt
    11581 gctactacaa tcggagcatc agatatatca gtatcatacc tgattgcagc agcatagtgg
    11641 gtaggattag ttattacaac atcagcctct ggcacttcat gcatcattcg tctcatagac
    11701 atttgtcttt gtttttgcct gattcttgac ttaacctcag gattaccttc aacctgtttg
    11761 tattcctctt taatctcctg ttttgtcatt ctgagattct tttcatattc ccaccattga
    11821 tatccataat ctgcaacccc gaaaagaatt agagcaaaac atatttttac tgcagcatta
    11881 atagaggtgt tataaatata tattcctgtt tgctttggct ccaattccat taggcttata
    11941 atatttgcag tttctccgtt taggtagcgg taagctatat aacctgttat gccaatttta
    12001 aacacagcct ttaaaagttc tactgctcct cttggtgaaa acaacctttt aaagccctga
    12061 aaaggattaa ttctatcaag tgtaagcttt attggttcgg cagtaaacaa aaatcctact
    12121 tgggaatatg cacctaaaaa acctgctgtt gcagatatta ccaatattgg ccctgtaatt
    12181 attgctaaac ttttcattac ttcaaggaac aataatgaaa caccctttat gttaaaatac
    12241 ccatccatat caagattctg agtcaaagtc cttactgtta tagcacttat ttccttgtac
    12301 attatattcc caaaagcttt taaacttaaa aaagcaaaca ttagcacagt agcagaagtt
    12361 atctctctgc tctggaatac ctgtcccttt tttctagctt cctgtctttt tttatgcgta
    12421 gctttttcag tttttgaatc tctatcttca gcaaatagtt gaagattcat aataagtctg
    12481 ctatttttca agtacatttt tccgttacct gccggccatt cctttcatta aattcaacat
    12541 ttctttatcc atatcataaa atagcgtttt aattaattcc atgagcatag gtatggttat
    12601 tacggcaaca ataaggccaa gagcgatttt caagggcata cctactacaa aaacattaaa
    12661 ttgcggcact gttctggata taacccctag aacaatatcg gcaattagaa ttgcggcagt
    12721 tattggtgct gcaattttaa aacccataat gaatatattc cctataattt ctataccgtt
    12781 acttgcaata ttatcgccat agcccaagct tccgcctcct gctggtatat atttataact
    12841 atcaaacagc gcttttataa gcatatgatg acctttaaac gagaagaagg ccaccatact
    12901 caatatgaaa tagaaatttg atgtaagaga gatttgtatg ctacttagtg gatctataac
    12961 attaacggca ccaaaaccta tttgcatgtc aataagctgt cctgcaacat aaattgatgt
    13021 aaacatggca tatgatacga aacccagtgt caacccggtt aagaactcaa acattacctt
    13081 caatacataa ttatatagca tatccccgta ttcaatgtta aaatcacctg ctgagtagta
    13141 gattattaaa gccattgaaa gagaaaagcc tatctttata taataaggta cattccgtct
    13201 tccgaataaa ggtgcaatta caaagaagcc tgtcattcgt acaaatatta ataaaaacat
    13261 ctccggcccg gacaggttta aaatttttag caaatccaac caaactcaac cctctctctt
    13321 aattatttac aatatcctag attactcagc aagacatttc taactgagag ataatcattt
    13381 tatataggca ttcatattta aataaatacc tcttgtaaat tccattaatt tatttagcat
    13441 ccatggcccg aatataataa tggccgctgc aacagctatt attttgggaa caaaagaaag
    13501 tgtctgctct tgtatctggg tggtagcttg aaatatgctc actgctaaac ctaccaacag
    13561 gcttaatcca agtataggtg ctgatatata aagcactgtc aaaagtgctt gttgtgcaat
    13621 attgctgatg atagcctgat ccattaacta atgctccttt ctgtgatttt ataaacataa
    13681 ttggtattat agtattacct tacaaagctt tctaatatag atctagccac cagattccat
    13741 cccccgacta aaatgaataa cagtattttg aatggaaggg atatcattac aggtggcagc
    13801 atcatcatac ccattgacat taaagtactt gatacaacca tatcaataat taaaaatggt
    13861 atgtatatta aaaaccccat tttaaaagct atcgttaatt catttataag aaaagcaggc
    13921 actactactg taataggtag tgcttcagga tccacaggtg tttcaactcc gcttagtgaa
    13981 acaaataatg ccagatcttt ctcatctcta atctgcctta acataaattt cctaataact
    14041 gacgtagaaa tattaattgc ttcttgttgg ccgatttctg tcctttcaag aggttgtact
    14101 gccctttcat tgatttctgt gtatacaggc tgcataataa agaacgtcag aaacaaagca
    14161 agtcctatta taacttgatt aggcggcatt tgctgtgtgc ccagtgcgtt tctcataaag
    14221 gaaagcacaa taacaacccg tgtaaaagca gtcatcatta ttattattga aggtgccaga
    14281 gcaagtattg taatcaaaag taaaatttgt atacttgttg aaacttcgcc agggttagtt
    14341 gcaggttcag caccaaagga aaatctaaaa gggagcaatg gttctgctaa tacattagaa
    14401 gcaggcaaag caactataac aactattaaa atagtagata tagtcaaaca atatgtataa
    14461 tttctgtttt ttatattcct tttcattttt gtgtcatcct tatcaagatc attttttctt
    14521 atattcaaac ccttgaaata aagctccaat cctatttaaa tttaccttaa tattgttaga
    14581 ataactgcca tctgcatttt cagaaatttc atccatgttg atttcatcag tactctttaa
    14641 cacctcaatc ccttttacgg acgaggagac aagaatgaat tcctctcctg ctttaattaa
    14701 atgcaaatat ctatctttac ccaggcatat tgtctcaata acacgaagat atttgcctct
    14761 tccaatttgt cctgctttgc ttccaatgta tttggttgaa atatatgcaa gaaacaatat
    14821 tgaggcaaat actaccatta accctaatac ataaataaat ttctccattt ttataacctc
    14881 tttagtcgaa atattacgca aaaataattt ttcacacgta ttttatccaa gagctctttt
    14941 gacagcttca acaaccctgt cagcttgaaa aggctttaca ataaagtctt tcgctccagc
    15001 ttgaatagat tcaataacca ttgcctgctg ccccattgca gaacacataa taattaaagc
    15061 gttgctatct attttcctta tttctttgac agcttgaatt ccatccattt ccggcatggt
    15121 aatatccata ataaccaaat ccggcgccaa atctttgtat ttttccactg cacgcaaacc
    15181 attctccgct tcaccagcaa tttcaaatcc ggctttggtt aaaatatcct tgatcatcat
    15241 tctcataaaa gctgcatcat caacaactag aatacgttta cccatttatg aacccccctg
    15301 tatcaaaacc tttttgatgg atgtacaata tccgtaattc taactccaaa actatcatca
    15361 attactacaa cttcaccttt cgcaactctt ttgccattta caagtatgtc aacaggatct
    15421 ccggcaagct tgtcaagttc cacaatggtt ccgtaattga attcaagtat ttcccgtatt
    15481 aatttctgtg ttcttccaag ttctacagtc acttgtaaag gaatatccat aactatatca
    15541 atgttctgct tttcgtcagt gaccgtttca tcttcaaaag attggaaatg caccggttgg
    15601 acatttactg ctcccttatc cttgtaggaa gaatcattct gtttaaaaga ttcatataaa
    15661 tcatttttcg gctgatattt atcttgattt ttttcctcat tttttatttc attataactc
    15721 tcagtttttc ttataatatt tgaacccgta tcctttgatt cctttacagt ttctggttct
    15781 ttatataaaa gattgttcaa taatgtttta gcaaatggta atggtaaaag ctgcattatc
    15841 tcactatcta tcaaatcacc aactaccatt ttgaatgcaa ccttaacaat cttcccgttt
    15901 tcaggactgt aaaaatcgtc atagtgattt agtgagtcaa acttaattat aaatgctgtt
    15961 ggaggtgaaa tatcaatcct cttattaaac atagttgaca tagaggttgc agaagatcct
    16021 atcatctgat tcatagcttc acttatagcg ctaagatgca tctccgtaag ctcaccatcg
    16081 gtaattctac ctgttcctcc catcataaga tccgttatta ttttcacatc atcttccttt
    16141 agtatgagaa gattggcccc ttcaagtcca gctgtatatt ttactcttat agcaacataa
    16201 ggaaggttat gttctttagc caattgttcc catgtggtta aggtaacctt tggggttgta
    16261 attgttattt tttgccctaa caatgtgaat agtgttgtgg ctgaagtccc tatagatata
    16321 ttgccaattt cccccaatgc atcaatttct tctaaagata attcctcatt atcctctgta
    16381 ttttgaatat tattaatatc agatacaggt atacctttta gtaaggcatc tatttcactc
    16441 tgggataatg cgtgttccaa tattattcat cctccttccc aaccaaagca gttattttaa
    16501 ttgagactct attcttttta acccctggtt tacctcggaa tttaagtaag tttcctacat
    16561 aaacatcaag ttcactatta atatcattgt ctagtggcaa tacatcacct ttttgtagtt
    16621 caataaaatc cgagactgta atgcttgttg agccaaaaat tgcttttaaa ggtactttag
    16681 tggctgatat ttttgattca atcaactcct ttgaagaact atctgtttgt ctctctatgc
    16741 tggtaaacca atagcgtgta cttaattttt ctattatagg ttccactgtt atatatggta
    16801 tacagatatt tatcatccct tctacatcag aaagctttat gctcaatgta accaaggcta
    16861 caacttcatt aggagatatt atcctggcaa actgtgtgtt ggtttcaatt ttttctaaaa
    16921 taggtctgat ttgaataatg ttttgccagg gctctctcat aacattcagc atctgaacta
    16981 ctatccgttc aataattgcg agctctattt ccgtaaactc tctttcatct tcaaatgtat
    17041 tacactttcc gcctagtatc ctgtcaataa aggcaaatac aatttttgga ggtatttcaa
    17101 tgataataga accttcaagt ggagtaaagt ctacaacagc caataccaag ggatttgaaa
    17161 ttgaacctat aaattcgctg tatgttaaag attccgactc tataacatca atctgtacca
    17221 aagctctcaa ataaccagtc aaaaaatttg tggccaatct agcatagttt tcatatatac
    17281 tttttaatgc tttcaagtga tccttcgaaa acttgctggg tctcttgaaa ttatagttaa
    17341 taattttttt ctcgccagat gcatctttta tatctttaac attaagttca ccggtaccaa
    17401 gagcttttaa caattcatca atttcattct gagataatat atctcccaaa tgttttcact
    17461 ccccactcta ttgataaaac caatcatcaa atacaacatc ataaataata tcattttgaa
    17521 ttttttcact tgagtttaaa aggtcattaa tctgattttt taaatcatct ttagccctta
    17581 ttttaaaatc aagattctta acctgatcaa ttgttacatt ctgaaagtat gtacctacca
    17641 actctctaat ctcactttca aagtcattga tttttttctc acaattttta attcccttaa
    17701 ctcttttaaa gtatctaagg cttattccta cacgaatagc cgattgtctt gcaatatttt
    17761 catttttgag aacaaagaac tgtttctcat caaataattt tttaatgcca agttcctcat
    17821 cagaaggacg tctgattaac tcattatttt cgtacattgt ctgcttttca ttgggtttag
    17881 cgccaagtac atatatatat ccaaccagca ttacaactgc catagttaat atagcaataa
    17941 ttacaagcaa tataataaaa ctgcctttgt tacccaaatt gattgccccc caattgtttt
    18001 aactcaaact ggttttacca taaaaactac cttttcatat ttaccatttc ctgaagcatt
    18061 tcatccactg tggtcataat tctgctgttt gcctgaaagc ctctttgtgt tattatcata
    18121 tcagtaaact ggcttgcaag gtcaacattg gacatttcaa gcgtaccggg gtttagcgta
    18181 ccggtacttc ctgttcccgg tttgatgccg cttctaaaga cacctgaatt tgatgtctgg
    18241 atatacatat tgttcccggt tttctgaagc cccgctggat tttcgaattc tgcaagagct
    18301 atcattccca gtggttttgg ctttccgttg ctgtaaatac ccatgattat tccatcattc
    18361 cctatactaa aagaggatag ctttccagga ggatatccat ctgaatttaa tggttttaca
    18421 gaactctcat cagcgaacat agtaagtttt cgtaaatcaa gttttacatc aaatgccttt
    18481 gaacctatgc tatctgcagg tataagattt attacaggag tgactctggg gttagtagca
    18541 tcaggggcat caagaatgat ttttccatca gagtcaaagc tgatttctcc actggctgtt
    18601 gttgtaagat caccaccttt tatgctccac tcccaggttg ttattccccc tgcagcttca
    18661 atttttctat atgccatggc tatttcatat tcgttcccaa gagaatcata cactatcatt
    18721 ggtactataa tctgtgcatc gtctccagct gcaactgtcg gatatcttgt atcaaggttt
    18781 cctgcaaaag aaatattttc agtggctttt gcggcaataa cctgcttatt cccattataa
    18841 tcgtcagtat ataggtttat aggctctaca ggcttattta cattaaagct aaaggtgccg
    18901 tcatcattca ttttctctcc gtaatccatc catccatata cactcatacc accggaagta
    18961 acaagatttc ctaataagtc ccttgtaaaa ttacctgctc ttgtaaacat aaatgtatct
    19021 gcctttgaac cttttactat aaaaaaccca tctccatcta tagaaaggtc caaaggacta
    19081 tctgttctct gctgacttcc tccggttatt attgtatcga tagatcctat gccggttccc
    19141 aaacctatct gcataggatt ggtccctcct cttcctaaag cagaatccgg cgcacttgca
    19201 ccttttaatg tctggttgaa aacttcttga aaggtaattc tgcctgccct gtaacctata
    19261 gtatttacat tcgctatatt attaccgatt acgtccatct tcatctggtg ggctttcagt
    19321 ccggaaattc ctgcaaacat cgatcgcatc atatatttat acctcctcat tggttatttt
    19381 ttcagcggtt tctaccattt taatactttc tattggtata tctattccat caacaactgc
    19441 ataggactct cttccgtcta ttttaacgcc ttctacttca cctgaaatag actttatttc
    19501 tccggttcta tcatcaataa tctctgcttt cacacttttg ccaataagat ttaaagcttt
    19561 cattgacgaa aaagagctat tcagattgta catctgttca agggtagtga actgtgccat
    19621 ttgagcaata aattccttgt cgtctacagg atttaaaggg tcctgatatt ttaactgagt
    19681 gacaagaagc cttaagaatt catctttacc caattcatta gtgtttcggt ttccattgct
    19741 atttgaatgt tttttaacta tttctgttgt atcttcaata ttaacaaccg aatttacctg
    19801 cataatacac ctccacaaat caatttcagg ccattaaatt tatcctgttg ccggaccaat
    19861 tccattggtt ataaccagta tcttcatttg aaatgccaat attatcagta atttcaacac
    19921 gagtttgaat tcttctgttc ttgttttcag gcattaggtt ccaatttctt tggttattgt
    19981 tataactttt atctgaatca tctcccgcaa tagatacact aaaaccttga actgtaagtc
    20041 cctgttcctt aagtgagtcc ttgagtgttt gaaaattgga ttccagaact tctttgacat
    20101 gctgattttc tgttataaac cttgctatta ctgcatcatt ttcagttatt actttcaatg
    20161 taactttacc taatatttca ggtttcattt ctataagcat ctcgtatttt tttcctgcta
    20221 taagaatcct agccttgtcc acaatttgtg aaattatttc atttgtttta ttttgattat
    20281 gggaaacagg ctgttcaagc ataatactac tagcttcttt agaaaatccc tctggaaata
    20341 acacccttgt aaaatctccg tctgtatatt catcttcttt tttaatagag gcacccttgc
    20401 tttgaagatc tgtttccggg cttgatagtt ctttattacc tgcatctgct aaatagcttt
    20461 cagaagataa tttaacattt agcatattga ggttctgatt attatcatca tcattcaatt
    20521 ccacgccggg tatattattt tcttcaacat atttaacacc actagaggcg ttaataatct
    20581 cagaatcatt cacaagatct tcttcagcta catgcttttc tgtatcattt tcagattgga
    20641 ctgcagcaac cttgcctgaa cggctttcaa actcaatttt cattttctcc aaaagctgtt
    20701 taatgttgtc tgtttctata tcattaacaa tatccttttt atctaaaata tcagttaaaa
    20761 tatctcggta ttcatcatct tcatataaga aatttataag ttctctaaaa tcaggttcaa
    20821 gcatattgaa ttcatttaca gcatcactct gcccttcatg aacctcgact aaatcacccg
    20881 agtaaatttg taataatctt tgcagaacca taagaggatc attaccaatt tctgaagatt
    20941 ctagccatat tttaacaatt tcttctttat gtgtgatatc ttcaaacttt tctccaatat
    21001 actccttgtg ttgaaaagta tggagaataa aggtatattc attattaatg ttggtctttt
    21061 tttcctgttt ccccttaatt tcattatcct ttttatcatt ttcctggttt gctttaattg
    21121 aacgtttagt cttgttatca acaagcaaat tacttttttt aattaaaggg gcatatttct
    21181 tgttacttcc caattcttgt ttttcgcccc ctgactgaaa ggatgtatcc ttttgtttaa
    21241 atataccaga aaaactcagt ccttgtaaaa gtataaactt atttgcaaca ggagttggag
    21301 attgagcact tctcacttct aatccggctg aaataccatt tgtcatgctc tcacctccct
    21361 tcacctgttt taagcattac ttttgacatt tcacgagtta cttttgatgc aaattcaggt
    21421 ttcatttccg ctaatattcc ggatgctttc tcctttttaa taccggataa aatatctaca
    21481 acaagcttaa tatctttttc acccatttcg atgaatatcc tggctgctgc cgcttcgtcc
    21541 atattttcat atatttgcag gaattttttt atatcctgct ctgttttttc atcttgcatt
    21601 atttgagcat atattttttc tgctttttct ttatctattg tttcaaaata ctttttaaaa
    21661 ccttctttat taccatcaac agccagcagc tgaatttctt ccttgctttt ctccagcttt
    21721 ttcatttcct ctgcaagata gtctttttct ttttccttat cttgtatact tttctcataa
    21781 gcctgtttat atataattaa acgattaatt tccaaattag cttcattcag ctgttttaac
    21841 aaatcatctc tttctatttt tatttctgag tatttttctc taatttcctt atcgttcaaa
    21901 tatttcggat catctgaatc cataggtttc ggtaatgccc atttaagcaa tgttatattt
    21961 tgtatctgtt cacggtattt ttctccaaga ccgtaaacat tgttatgtat taccatatag
    22021 aaggcaccac ctataattat gcacataata actaatgcag taaggattac cagcaaagtg
    22081 aataatacat ttttgctcat tacatatcct ccaccagttt catattctga cggtagctta
    22141 taatttcatt gttaaacttt tgctcctctt taagcaattc cttattaaat aatatttgct
    22201 ttttattttt taatttttcc agcatttctt tttcctgtga aactttaatt agctcctctc
    22261 gtattttatc gacattttct ttagctttat ttatactttc tttttgcatt tctatttttt
    22321 ctttctgctt tccgaaataa atattaaaac tattcatatc agtcgtcttt gctccaacat
    22381 aaagcaacat cctataatct tgcatatatc tactgctttc ccttgtaagc gcatcaagtt
    22441 ttttattttc catatccaat ttaccaagag cttcaccaag ttcaattttc aggatatcct
    22501 ccgcttgctc tttaatgttt agaacagatt ttaatctgaa attgaatttc ataattaatc
    22561 aaacaccttc tccaacatat ttaatacatc atcaaatgta taatttgtat ttgtatcctg
    22621 ctgaatgaag tttaaaatac tctctatatg atcaatggca aaatcaatat tattattgct
    22681 tcctttaaca taagcgccga tatttattag atcctcagca tccttatata ttgccattac
    22741 cttttttatt ctgcctgaaa tttttttatg ctctccagat acaacatcag gcattaccct
    22801 gcttacgctt gccagaacat ctattgcagg gtactggttt ttgttggcaa gttcacggga
    22861 taaaactata tgcccatcca ggatccccct tgcagtatcc gtaacaggtt cggttaaatc
    22921 atctccatcc acaagcactg agtatagtcc agtaatagaa cctttatcgg aagtaccggc
    22981 tctctctaaa agttttggca gcactccaaa cacagatggc gtataaccct ttgaaaccgg
    23041 aggctctcct atggcaagcc caatttccct ttgcgccatg gcaaaacgtg ttaaggaatc
    23101 catcatcagt aatacatctt ttccctgatc tctaaaatac tctgcgatgg acgtagctac
    23161 ctgagcgcct tttagtctta ttaatgcagg ctggtctgat gttgcgacaa ctacaaccga
    23221 tctcttcagg ccttcttcgc caagatcctt ctcaataaat tccctgactt ccctgcccct
    23281 ctcacctatt aaagctatta cattgatatc cgccatagtg tttctggcta tcatgcctat
    23341 aagtgtgctt ttaccaacac cactccctgc aaatattcca attctctgcc ccttgcctac
    23401 cgtcaacaat ccgtcaattg cctttattcc tagagacata ggttctttaa ttcttttacg
    23461 cattaaagga tttgggggtt gattatcaac cgtataacgc tcttttgtat caatataacc
    23521 ctttccatca ataggtgtgc ccaaaccatc aagcactctt cctataagct cttctcctac
    23581 tttcacattt aatgttcttc cctctgaaat aacaagattg cccgggccaa ttcctgtcat
    23641 atctccaaat ggcattaaca gaacgttgtt atctttaaag ccgacaacct cagcatatat
    23701 ttccctatca tatttcatag tgtgtattct gcataattca ccaatatttg cttcaggacc
    23761 ctttgattct attgttaatc caataactct tgtaacactg cctgaatatt caattaatgg
    23821 aattttatcc agtatatctt tatacttgcc aaggttaata ggtgacaatt aaagtacctc
    23881 ctgtatatca ttaaagactt tttttatttt atctaatttc atttcaatac ttgtatttat
    23941 actgccttga ggtgaatcta ttatacaccc ccctgctttt aaggagggat cctttcttat
    24001 gtcaaaatca acagcttccc cgaatagcat atcgaaactt ttactgtttt caattaaata
    24061 ctcataatca tcaggcgata ccctcagaat cagctctttt ttctccgaac acttatcaaa
    24121 agcttctttt gtaagaccaa gaataatatc tttattatag gttatttcat gttgcagtac
    24181 tttttcagca atatcaagaa ttacgcctac agcatccggt tctgcctcag caagcatttg
    24241 acggtaatcc tcccttgctt gtttctttat cagatccaat tctgcattaa ttttttcaaa
    24301 tttttcggca gcttcacttc gaccaatatc aaagccctca ttatatccct cgctataacc
    24361 tttcttcctg gcttcttcaa tcgctttact gcatttttct ttttctatac gagaatcatc
    24421 aataacgcgc tggtattcac ttttagcttc tttaataatt aagtcggcct gtctttgggc
    24481 attttcaata atttctttag ttctgaattt attaactgca tatccctttt caccttcaat
    24541 tataaaagga gttgaaatgt atcctttgtc cagttctttt atcctgctct cttttatgta
    24601 ttttttttcg ttgacacaca attcctcttc aggtatctcc acaacgaagg gttttcctat
    24661 acaaacctta cagtttttaa ttactttact agacaacaat ttcatctcct cctcctctgg
    24721 atataacaat ttcaccggct tcttcaagtc tgcgtattac attgacaatt ttttgctgtg
    24781 cttcttctac atctcttaac cttacaggtc ccataaattc catatcttcc tttatcattt
    24841 ccgacatacg ttttgacatg tttgcaaaaa ttatcttatg tacatcctct ccagcaccct
    24901 ttaaagcaac aacaagctga ttattgtcaa tttcctttaa gaatctttga atagacctat
    24961 tatcaagggt aagaatatct tcaaagacaa acatgcgctt ctttatttct tcagcaagat
    25021 cagcatcacc tatttcaaga gtttccatga tatatttttc tgtacttcta tcaacattgt
    25081 tcagaatatc tacaatagtc tgaacaccac cagatgaggt aaaatcatca atagcaatag
    25141 aggaaagatt cttttccaga atcctttcta cctctttaat aatttccggc gaagttctat
    25201 ccattaaagc aattctagaa gctacatccg cctgtttatc ctgtggaagt gcagacagta
    25261 ttgcagcagc ctgctgaggt ttaaggtagg aaagtatcaa agcaactgtt tgaggatgct
    25321 ctttctgaat aaaattcata agctgttcag gatcagcttt ccttacaaaa tcaaagggac
    25381 ggacctgtaa agaagctgtt agtctgttaa ttatttccag agttttttgg ctacccattg
    25441 ccttttccaa tattttctta gcatattcaa ttcccccttc cgaaatatac tcctgagcaa
    25501 ggcatatctg ataaaactct ttcagcacct tctctttttc ttctggtgaa acaaaacgta
    25561 tatttgctat ttcaagagtc agctgttcaa tttcatcttc tctcaaatgc ttgaatatct
    25621 cggaagatct ttcagggcca agggatatta ataacatagc tgctttttct tttcctgaac
    25681 gctccctttg tatacttctt accatctata tccctcctag gaatcccttg tatcggaaag
    25741 ccagtttctt atcaattgtg cgattgcttc cggtttatct ttggcaaagt catcaatctt
    25801 cttttgaatt tttgattttt catccagttt aacctcattt atatctttta ggtattcttc
    25861 tattttttca ggtaccatat attccgattt agtttcatca ttctcaatcg ccgctgattc
    25921 atcttttttg ttatgttttt tcattgatat aaccgttatt gttaatccta ttatcaataa
    25981 gcctaatata ataagccata aatagtcttt aactataatc tctttcaaat cacctgtctg
    26041 tatttgagcg gaatcttgcg ctgccagctt gtacttactt acagatatgt tgttttcagg
    26101 tattcctgta gccatgctcg ctgcaattct tatctggtct ataaattcat cagaaattcc
    26161 atttccgtca ataacacttc tgccatacca tagagatatt gccatggaag acttatctgg
    26221 tatcaaatat cccaaggctt tttcctcttc ttttaaagct tcatcatatc cgaaattttg
    26281 ttttccctct tttaagctat atgttgaatt tccatcgttg cccaaaagat atgttggaga
    26341 acctgcttct ccgggatttg tatcagtacc gggtatatct cctcttgata cattttccgt
    26401 tttttccgtt ctttcttcac tgcttataac cgctcctcca tccatatcgg aagggtttgt
    26461 aatattcttt gattgagttt taactttatc gaaatccaaa aaaggattta ctacaactct
    26521 caaagtatca aaattatcaa actgacctac gctaaaataa ctataaacct tggcttccaa
    26581 ctcctttgct tttttagtac gcatttcttc ctggctggtt agaactgtta tttcgttgtc
    26641 cggggcatag ctgttcaaca aattcagatt attgtcaaca actgttatat cttttggatc
    26701 aagcctttcc acactttttg aaacaagcat gactataccc tgaacttgct cctgtgtaat
    26761 gtaatttttg ggttttacca taataaaagc cgtaggttta tactcttttt tatcagaagt
    26821 taaaaataca gattgttctg gtaaagcaag gcttacttca gcatattcga tattatcatg
    26881 agccataatt tttttagcta tttcattagc ctgctgctgt ttccaaatat gcttcttatc
    26941 attctctgta gtagttatgc caatcatctt aatggcatct tcaaaggtca ttccgccttt
    27001 gggataacct ttttcagcca gcagaatttg agctctgcca ttatctttag agttaacaat
    27061 aaggctttcc ccatctttcc ctacatcatt ccacaattta ttctcagtaa gtattgaact
    27121 catctcacct atttcctttt tgcctgcgcc acttacaaca gttgtatatg aaggctttgt
    27181 taatgtggta ataccaattg ttactgctac aataacaata gacgatgtta tgtaaatacg
    27241 ggttttatgt gacttttcca atcctgccca gaactctttt atctgcttaa ttaaccttga
    27301 tatcatatca ggcattttat aagttacacc gcctcaaaat ttccttattc cttatatttg
    27361 catcctcatt atttcattgt aagcatccat aatcttattt cgtatctgca aagtaaattg
    27421 aagtgctata tcggcctttt ctacttcaac aagaacctta tgtatatcat ctatatttcc
    27481 aactgcaaat tggttactta tattttcagc cttcactata atatcatttg ttttgtttac
    27541 agcttcttta aaataatctg caaacccctt tattccatct tcagttcttt gtatacctgt
    27601 ctttttcaaa gaagatatgg aaagagattt gtcaatggca cctatatcca ctttggttat
    27661 ccccctttat tatgaaaata ttaattttta ttcatactgt agcaaaactt aaaaatcata
    27721 tatttgaatt cagcatattt attttcctat ttcaagagct ttaagagcca tacttttagt
    27781 tgcattgatt gaggttacat ttgcttcata tgcacgagat gccgatatca tatttatcat
    27841 ttcagtaata atgtcaacat tgggcatcag cacataacca tcctgatccg catcagggtg
    27901 gcctggatcg tatattcttt taaaaggtga tgtgtcttct gcaatatgtg taacccttat
    27961 accattctct gttttaagaa acttatttct gctgctttct gaaagaaaat gtgaaaatgg
    28021 catattatta tttctttccc caaatagaac gacttttctt ctataaggca tgccattgct
    28081 ggtacgtgta gtattaatat ttgaaatatt ttgggatatt atatccattc ttaatctttg
    28141 agcagttaaa ccagaagtcg ctgcacccag tgaatctaaa aaacccataa cttatcttct
    28201 cccttcacta ataacggact ttagcatctt caaacttgag tttatactct gaataaggac
    28261 attatatttt atagtatttt tagccattga agctgattca ttatctatat ccacattatt
    28321 gccgtcaagc ctggtgctta aatttttgtt atctgtcaac atccttattt gcatgttatc
    28381 aatgttatca atgttatcaa ctttatcttt tcctttttcc agatgtccgc tatctgtttt
    28441 caagccttcg gaattattgt ccagggcatt tttcagtaac tcttcaaata tgacagtctt
    28501 cctcttatag cccggtgtat caatattggc tatattttgt gatatggctt tatttcgcag
    28561 ccatgcagca tcaagtgctt tttcagtaaa tttaatattg ctaaatattt tatttaccaa
    28621 acgaatcaac ctccttacaa tttgaaagca acatactgtt tctaattaac ataaatgtaa
    28681 aattatgcat caagctatat aaatattagc ataaactgta gaaaaataca atataggtct
    28741 gaagattttg taaaataaac ttgtatagaa agaaattttg tcaacatatt gtgacaaaaa
    28801 aataagccac ggggagctgt ccccggtgac tctttcaaat agagggtgca tataatttaa
    28861 gtgaagaaaa tgcttactta ttcattttct ttctttttgt ctctagaata actgcatttc
    28921 tcattactac attttatctg actctttgaa ccgctatatt taattaccag gaagcttcca
    28981 cattcaggac aattctctcc tgcaggctta tcccaagtca taaaatcaca ttttgggttg
    29041 ttttcacaac cgtaatactt ctttcctttt cttgtcttct taactaatac tttggcttga
    29101 cattttgggc ataatactcc tgcttcttca actatgggtt ttgtatttct gcattccgga
    29161 aaacccgggc atgcaagaaa ttttccataa cgccctgttt ttataaccat attttttccg
    29221 catttttcgc attttacatc ggtttcttca tcaggtattt ctacattacc aatttcaagt
    29281 tcagcttgcc ctatagtctt atgaaaatcc gcataaaaat ctttcaaaat atcgacccag
    29341 tccttttcac cttcttctac atcatcaagc tgactttcca tttgtgcagt aaattgtaca
    29401 ttaactatgt tctcaaaatg attcttcatt ataccagtaa caattctgcc cagctctgta
    29461 ggtaaaaggt ttttcccttc cctgacaaca taatctcttg atattattgt acttattgta
    29521 ggagcataag tacttggccg gcctatgcct ttttcttcca gtgcttttac cagagaagct
    29581 tctgtatatc tttgaggagg ttgggtaaaa ttctgttcat gctctatctt ttttagagaa
    29641 agcatttcac cctctacaag ctcaggcaga tttgaattat cttctccctg ttctgcattt
    29701 ttttgatctt tatcatcatt atcctccctg tataccatgg tatatcccgg aaattttatt
    29761 cgtgaaccgg atgccttaaa aacacaatct gccgctaaaa tgtcaacatt taaagtatca
    29821 taaactgccg atgacatctg acacgcaaca aatctttccc atattagctt ataaagcctg
    29881 tattggtctc tgctcaagga ttcctttaca gaatcgggca acagtgatat attggacggt
    29941 cgtatagctt catgggcatc ctgggaagat gacttgtttt taaagactct tgacttctca
    30001 ggggtatatt ccttgccata cttgctatct ataaattccc taacttcatc aagggcttca
    30061 tttgatattc ttgtggaatc agtacgcatg taggtaatca atccaacaga gccttcaccc
    30121 ttaatttcaa ctccttcata caactgctga gctaacatca ttgtcttttt tgtggtaaat
    30181 cctaattttc tggatgcctc ctgctgcaaa gtactcgtta taaatggtgg cgcaggggat
    30241 ttttttcttt ctcctttttt aactttgttt acataaaaat cctggccact gattttgtct
    30301 aatatttttt caacttcact tttatttttt aagtcaacct ttttatttcc tactccgtaa
    30361 aatcttgcct taaataatgt tttagaattc tttttagtta agtttgcagt aattgtccag
    30421 tattcctcag gaatgaactt ttcaatttct tcctctctgt cacatatcaa tctaacagca
    30481 actgattgaa ctcttccagc acttaacccc tttctgacct tcttccataa aatgggactt
    30541 atcttatagc ctactattct gtccagaact cttcttgctt gttgagcatt aactaatttc
    30601 atgtcaattt ccctgggaac ctttagtgaa tttgttactg cattctttgt tatttcatta
    30661 aaagttattc tgcacttgga cacttcatca acgtcaagca aatttgccag atgccaagat
    30721 atagcctcgc cctcacgatc cgggtcggtt gccaggtata cacgttctgc tcccttagct
    30781 tcttttttta attttgaaat cagttcacct ttgccccgga tggttatata tttaggttca
    30841 aaattatttt caatgtctat ccccatatgg cttttgggta aatcttttac atgtccggca
    30901 gatgccatga catggtagtt tttacctaaa aatttcccta tacttttaac ttttccggga
    30961 gattcaacaa taatcaattt atatgccatt ctacccctcc atccacgcct acgactgtta
    31021 ttttccgcca atcggattgc ttcacaccgt ttcaataaat acattaacat aaaaccattc
    31081 ggttttattc ccttggtcgt atttattata aacacattaa ttacgtttag tcaaatagga
    31141 ttttcatatt tttaatttat atattttgcc cggcaactgt tcaataattc cctttagctc
    31201 cagcatagtc aatatagaat tcgtgtattt aatatcataa ccggcttttt tcgctaaaag
    31261 atcaacatac atagactcac ttttcaggca ttctacaatt cttctttctt ccttgtcaag
    31321 tcctttatac ctggtttgct caattggatt ttttatttta ttttctcctt ccagattgta
    31381 tatcgagagt tcctcaagaa tatctttaac actcataaca agtttagccc cttctttgat
    31441 tagcatattt gtcccagtgc tgttaatacg gttaatatta ccaggcaggg caaaaacttc
    31501 gcgcccttgt tccagggcaa agtttgcggt tattagagat ccgctgtcag cagctgcttc
    31561 aacaactaca acaccaaggg acataccact tataatcctg tttcttgcag ggaaattcct
    31621 ttggaaaggc attgtccccg gtaagtattc agaaataacg gctcctttct ccaatatatt
    31681 attcatcaat actttgtttt ctgccgggta tacaacatca agtccacacc ccaatacagc
    31741 aatagttctg ccctgtgctt ttagtgcacc tttatgtgcg cttgaatcta tacctcttgc
    31801 cattccgctt actacagtta ttcccaggca tgccagctga taagctaatt cttccccggc
    31861 ttcaaggcca tatgctgttg cttttctcga acctaccaat gaaattgcat tttcatttgc
    31921 taaaattttg cccttgcaat aaagtacaac aggagggtca tatatgtttt ttagataata
    31981 tgggtacaac tcatcatgaa tagttattac ctctatttgg ttttttctta ttgcttctaa
    32041 atgtttacat acattgtttt tatgtttcac atcaattagc tgttgtatta aagcctctgt
    32101 aagaaaaggc aatattctta actc
//
LOCUS       DAOSDD010000021        31433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_698_length_31433_cov_4.906559, whole genome shotgun sequence.
ACCESSION   DAOSDD010000021 DAOSDD010000000
VERSION     DAOSDD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 31433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31433
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_31433_cov_4.906559"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..3106
                     /locus_tag="PK733_03455"
     CDS             83..3106
                     /locus_tag="PK733_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ09631.1"
                     /translation="MNSTITKLSSIKGRTSSYWGERIVSQLEYGVKLSRVNDRSYDEL
                     IGTAVKFLDNKLAEEGVITKQVALETEKMLAGMSEAAKKLKVICAAHAHIDMNWMWGY
                     AETVAVTLDTFRTMLNLMKEYPEFTFSQSQASVYKIIEEHNPDMLEEIKKRVKEGRWE
                     VTASTWVETDKNMPNGESLARHILYTKKYLSELLDICPDSLKIDFEPDTFGHNLNVPE
                     ILNNGGVKYYYHCRGYDKRVVYRWKSPSGNSILVYRDPFWYNAEINPSLALYVPEFCA
                     KHGIDTMFKVYGVGDHGGGPTRRDIDRIIDMASWPVFPQIKFGTFTQYFAILDTIIDK
                     LPVEDKELNFIFPGCYTSQSRIKMSNRIGEAKLNEAEAFSTISAAFAGGKYSSKALEE
                     AWRKVLFNHFHDILPGSGVIDTREYAMGKFQEVLATANTEIYNAFRNIVSQADTTGLL
                     TFEEDITYTMSEGAGVGFAIKDFGVPQTERGKGKGRIFHFFNSSAYERIEPVEIVVWD
                     WPGDKNRMFIKDASGNPARYQLLKNDKYQFFGESYWGHTYIRVLVDVKVPAYGYTTYT
                     LIEKEMDDLPVALAKEPRVEEENAYILENDHIKVTFDTRNASIISIVDKKSGEEMVSP
                     NRPAGNFRLIEEDDSKGMTSWVVGRYMQIFNLNEMKNVKIRSYYGEKSALRQWISYSI
                     EFRASKLNVTVSLDYNSSRLNYVVECDWQEKPEKGKFVPQLNFYMPLNYECRRYKYNI
                     AFGTIEREALNMDVPANSWMAGISACNSNNYSRLDCKDGCGKMLMLITDTKYGFRGYE
                     NSLAVSLIRSSYDPDPYPESGIHKFKFAVDVVDISFDENAINNTLIRKAYEYNHPVHF
                     ISGTIHEGTFPIENSFISLESGTVAISAVKMPEVNRIDIQQKESRSEGSCSCCEPCGE
                     IISWKSAILRAYETEGRKTRAVVKFAEKVAEAFFVDINEKPLEYKVKDNNSGNECCID
                     NCNGVDIDGVNIAFDVNGYSVASVYVKFETEEAEE"
     misc_binding    3180..3487
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3578..4597
                     /gene="pheS"
                     /locus_tag="PK733_03460"
     CDS             3578..4597
                     /gene="pheS"
                     /locus_tag="PK733_03460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ09632.1"
                     /translation="MREQLINIREKAKEVISSVRSMQELDNVRVKFLGKKGELTPILR
                     GMGSLSPEERPVMGQLVNEVRTSIEELLEHAKNDLAQKEKSLKLQLEVIDVTIPGKRK
                     NLGSKHPLSIVMDEIKDVFIGMGYEIAEGPEIELDYYNFEALNTPPGHPARDVQDTFY
                     INDNILLRTQTSPVQVRVMEKHKPPIKIVCPGRVYRSDAVDATHSPIFHQMEGLVIDK
                     GVTMGDLIGTLQVFARSLFGENTKIRLRPHHFPFTEPSAEVDVSCWTCGGTGCRVCKN
                     SGWIEILGAGMVHPRVLEMSGIDPEIYSGFAFGIGIERTAMGRFNIDDLRLLYENDLR
                     FLKQF"
     gene            4692..7091
                     /gene="pheT"
                     /locus_tag="PK733_03465"
     CDS             4692..7091
                     /gene="pheT"
                     /locus_tag="PK733_03465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ09633.1"
                     /translation="MNVPIKWLKEYVDINVSVEELCDALTMSGSKVENIEKQGEEISK
                     VVVGKILSIEKHPDADKLLVTKIDTGDETIQVVTGAHNISEGDYVPVALHGSTLPGGI
                     KIKKGKLRGVESNGMLCSIQELELTKGDYPEADEDGIFILRNMENLKLGQDIKELVGL
                     DDTVVEFEITSNRPDCLSVLGIARETAATLKTNLKKPVINVKEEENDKAYVYAQVEIR
                     NPELCSRYAARVVKDVRIGPSPEWMRKRLRAAGVRPINNIVDITNYVMLELGQPMHAF
                     DLSYIKDSKIVVRRAEDGEVMKTLDGQERKLDSSMLVIADSEKPIAVAGVMGGENSEV
                     NPNTRIILLESANFNGISVRLAAKKLGMRTEASARFEKGLDVENVITAVNRAAQLIEE
                     TGAGKVCWGIIDCFPGKKNKRVLKLRPEKINAFLGTSISLDTMIEILESVEIRVDKDT
                     MTVIIPEFRQDLEREADLAEEIARLYNYNNIEATLLKGKAATIGKKTTKQKIEDIIKN
                     TMMSCGLSEICTYSFTSPRVFDRIKAASNSELRKAIPISNPLGEDYSIMRTTTVPEML
                     GVISRNYSRRIEKAGFFELAFVYLPEELPLKKLPLEKEVLTLGMYGDTDFYDIKGIVE
                     ELLYTLKIKDYKVVPESGNDTFHPGRTAKIIIDGEPAGIIGEIHPDVSEEFGAPERTY
                     IGMIYVEPLIRKTVMEVQYKQLPKHPAVTRDIAILLEEEIMAGDIEQVIKENGGKIFE
                     SIELFDVYKGKQVPEGMKSMAYSITFRADDRTLTDDEVNKGMEKIVSALSDKFNAKLR
                     E"
     gene            7170..8516
                     /locus_tag="PK733_03470"
     CDS             7170..8516
                     /locus_tag="PK733_03470"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOJ09634.1"
                     /translation="MDKISFDYSKADKLVAQHEVQYFKDFVKHAHEMIHSKTGAGSDF
                     LGWVDLPLKYDQEEFVRIKKAAEKIRSNSDALVVIGIGGSYLGARAVIDALSHSFYNL
                     LDKNKRETPEIYFAGNNISPKYLSDLLDILDGKDVSVNVVSKSGTTTEPAIAFRVFKE
                     FMEKKYGRKGAAERIYATTDKSKGAMRKLAEEEKYETFIIPDDVGGRYSVLTAVGLLP
                     IAVSGLDIDALMDGAAAACRQYNNDNLNENECYQYAAVRNALYRKGRTTEIMVNYEPS
                     LHYFTEWWKQLYGESEGKDQKGIFPAGVDFSTDLHSMGQYIQDGLRNIYETVLNINKS
                     GIDITIKEDKDNVDGLNFLAGKTMDFVNKKAMQGTILAHTDGDVPNLVITIPELNTWY
                     LGNIIYFFEKACGISGYLLGVNPFNQPGVEAYKKNMFALLGKPGYEDEQKRLECML"
     gene            complement(8681..9616)
                     /locus_tag="PK733_03475"
     CDS             complement(8681..9616)
                     /locus_tag="PK733_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ09635.1"
                     /translation="MKQFKGNLILLITAIIWGTSFASQKLGMDHIDPFTFGASRFLLG
                     AVVLIPAILIFDALNRSKKNIFFAEANKPQFKNKDLIIGGLLCGGTLFLGASFQQWGI
                     VYTTAGKAGFITTLYIVLVPLLGIFTGKKTDLPTWAGIALAIAGLYLLCIKEGMSMQK
                     GDVIVLIGTIFWALQIIVVDAYADKVEALKLSLVQFITAGILSAIAAVIFGNPDIHAI
                     VDCAGPILYTAIMVVGVAYTLQIIGQRYTNPTVAAIILSMESVFAAISGAVFLNEIMS
                     PREIIGCVLMFTAVIITQVNTQEILGVFRRLPVDK"
     gene            complement(9680..10150)
                     /locus_tag="PK733_03480"
     CDS             complement(9680..10150)
                     /locus_tag="PK733_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOJ09636.1"
                     /translation="MSQYIVNLLKIIFISAVPLIEQRGAIPVGILAYKLSPIVVFAAS
                     FLGSLLPVPFILLLFNVIFEWMKKFKFLSKLNDFIENKVRKGSVKVERYKELGLIIFV
                     AIPLPTTGLWTGSAVAAFMGLDFKKSFLCVAIGGIISAIIITLISILFPAMLGY"
     gene            complement(10300..11172)
                     /locus_tag="PK733_03485"
     CDS             complement(10300..11172)
                     /locus_tag="PK733_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ09637.1"
                     /translation="MIYIYSFLLFILIMLLYMRFEAGWLKVDRVKFTLSNKGLKIIQL
                     SDIHMKYLKVSAQKVKNVISREKPDIIIMTGDYIENQDQIDAFLHFLETIRTIKSDTV
                     IYLCMGNHDYKAFDRNCGKYHRQFHCKPHGHSKYQSHNIEDFIQSIESLGVTVLYNRA
                     VSYSKNSVKYNIIGFDDVNAGHPDINKALSFCAEDAFVNIAFSHNPDIIFQIPKGKVD
                     YLFCGHFHGGQIWMPFHLEFNLLRKEKLCRIGINRGLHKLNGINLYINRGLGNVCFPL
                     RFLSRPEITVFYLP"
     gene            11381..11743
                     /locus_tag="PK733_03490"
     CDS             11381..11743
                     /locus_tag="PK733_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ09638.1"
                     /translation="MNSSFGDRCEICEVHKDKTECMKKCMLDESNAVRLSEVFKVLGD
                     QTRIKILYVLSKCEMCVCDIAEVLDMSQSAISHQLRVLRTMRLVKYRKEGKSAIYSLD
                     DDHILQLFIQGMEHIKHS"
     gene            complement(11761..12321)
                     /locus_tag="PK733_03495"
     CDS             complement(11761..12321)
                     /locus_tag="PK733_03495"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOJ09639.1"
                     /translation="MFIIYAVIIGILIGYISGGRLKFLAQNPFKHIWLVISGFLIQIL
                     IFSDISIFKALQQNSAVLVTFHILSYILILAFTAINIKLPGVAFIGTGILLNAIVIIL
                     NKGHMPASIQSFESASVGRYTEILSRGETINNSKAIADDTLLPWLGDIFAIPSYIPFS
                     NVFSIGDIIIAIGACIYFAYGMKPKK"
     gene            complement(12328..13728)
                     /locus_tag="PK733_03500"
     CDS             complement(12328..13728)
                     /locus_tag="PK733_03500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOJ09640.1"
                     /translation="MRNHFLQIKNVLKRFNLKYFYVYLIGFSVFPLISYSTPHINFKG
                     WFHILFWIILELAADLKPFRSIFYLRMDMTLSFSVQLAMIILLNTWEAVWIVIVATII
                     AELISKKEWYKAMFNAGQYGLSLLVTSFVFNLLKMSPNDIPLDIIKDLPAIIASVIVY
                     YFMNTLFISIVISLFYGNKFIDVFFNDYKIMAAFYFSITPISIAASLLYQESHPYKAL
                     ILIPPLIMADQSIRRYYSLHMETIETLNVLADIIDERDSYTYSHSSRVAEYSGKIAQE
                     LHLSSDAINEVETAGRVHDVGKIAIEDSILRKDGKLTTEEYDNIKKHPEVAYRLLKNL
                     KPYKNGAKYALYHHEKIDGTGYPENLSGRSIPLGARILAVADCYDAMTSDRPYRKALP
                     QSTAIKELLKYSGAQFDPVVVKAFIEVLKRDYGYFDMDNNHTSIDDKSDNEYTVTDDK
                     TNDKCNNINDKGEIRR"
     gene            14449..14601
                     /locus_tag="PK733_03505"
     CDS             14449..14601
                     /locus_tag="PK733_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09641.1"
                     /translation="MENGKGFLGRLFGNDMILWFIILFLLLFWCTNCFRADNRENDVD
                     LGLAHE"
     gene            15213..16256
                     /locus_tag="PK733_03510"
     CDS             15213..16256
                     /locus_tag="PK733_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ09642.1"
                     /translation="MIKLEMEAVDPLPGGHRYNVGLTYNLKKGLKSDVEDIEAELDSF
                     DTVNAIKEALEKLKCNVTLLEADESVIPNLLKARPDMVFNIAEGLNGRGREAHIPALL
                     NFLGIPFTGSDETTLCIALDKTLTKRILSTYGINTPMYCLIKNPSEKLPVNMKFPVIV
                     KPNAEGSSKGISDMAIVKNMEQLKGLIEKNYEMYKQPMLVEEYIKGREFTVAVIGNSD
                     DAKVFPPMEIRFKRQEEQYSIYSYNVKKNYKEYIEYICPPMIDENILQKMVETARKVY
                     ECLDCRDFSRIDFRVSDEGEVHFIEINPLPGLAPGYSDFPMIMEFNGESYDNTIKMVL
                     NSALRRYGMNTVK"
     gene            16271..17383
                     /gene="ablA"
                     /locus_tag="PK733_03515"
     CDS             16271..17383
                     /gene="ablA"
                     /locus_tag="PK733_03515"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOJ09643.1"
                     /translation="MRDYKTVDIWKDVDIEQWEDWKWQLKNRIVTAEQLGKVISLSEK
                     EMDDINLCLKMFRMAITPYYSMLINPDDDNCPIKAQAIPSIKELNIRSDDLSDPLGEE
                     FYSPVENIVHRYPDRVLFLLTHKCSMYCRHCTRRRVVGEEDFSVNEETLDKAVDYIKS
                     NSKIRDVLLSGGDPFIMSDSYLEHIIQRLREIPHVEIIRIGTRTPVVLPMRITDSLIN
                     MLKKYHPIWINTHFNHPMEITEASVKACAKIVDAGIPLGNQSVLLKGVNDNVETLKEL
                     LLKLVKARVRPYYLYQCDIAQGIGHFRTKVEEGMEIMKNLRGYISGYAVPTFVIDAPN
                     GGGKIPINPEYVVSMDDERVVMRNYKGDIYFYPNAG"
     gene            17759..18604
                     /locus_tag="PK733_03520"
     CDS             17759..18604
                     /locus_tag="PK733_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOJ09644.1"
                     /translation="MFKIVKKGVLNPAVKLLEVEAPYIARKAEPGQFIILRVNEEGER
                     IPLTIADFNREKGTVTIIFQEVGKTTQLLGALNEGESILDFAGPLGMPTHYGEVKKAA
                     VIGGGLGTAIAYPQAKKLHSMGADVDVIAGFRNKDLIILEEEMKAVSSRTFITTDDGS
                     NGNKGFVSDVLKRLIEEGNKYDLVVVIGPLIMMKVVSGITKEYGIKTIVSMNPIMVDG
                     TGMCGGCRVTVGGKVKFACVDGPDFDGHEVDFDEAMRRQLMYKKEERHSMEGHRCRLE
                     GIKNA"
     gene            18597..19988
                     /gene="gltA"
                     /locus_tag="PK733_03525"
     CDS             18597..19988
                     /gene="gltA"
                     /locus_tag="PK733_03525"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOJ09645.1"
                     /translation="MPNMSLKKMQMPEQEPEKRVKNFLEVALGYTEEMAVEEAKRCLN
                     CKHKPCTKGCPVNVKIPEFITLVAEGKFEEAYLKINETNSLPAICGRVCPQESQCEKF
                     CVRGKKGEPVGIGRLERFVADWYMANRKIEQTQVERKNRKVAVVGSGPAGLTCAGDLA
                     KMGYDVTVFEAFHTPGGVLVYGIPEFRLPKELVNKEIDTIRNLGVDIKTNMVIGRVLT
                     LDELKKEGFEAVFIGTGAGLPSFMGISGENLNGVYSANEFLTRINLMKAYKFPDCDTP
                     VKVGRNVAVVGGGNVAMDAARSAKRLGAENVYIVYRRSEEEMPARLEEVHHAKEEGII
                     FKLLTNPTKIIGTEDGWVKGMECISMELGEPDSSGRRRPVPKAGSEHILDLETVIIAI
                     GQSPNPLIKSTTPDLETQKWGGIITDEETGATSIEGVYAGGDAVTGAATVILAMGAGK
                     KAAAAIDKYLSER"
     gene            20071..21027
                     /gene="hprK"
                     /locus_tag="PK733_03530"
     CDS             20071..21027
                     /gene="hprK"
                     /locus_tag="PK733_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOJ09646.1"
                     /translation="MSEEYSVTLKEIVDDFQLEIISGHNRIYDVKITTSDINRPGLQL
                     TGYMENFGSERMQIVGMVETSYISGMTSDERYKRLDAYFKLGFPCVIVTRGLDVFEEM
                     IEISNKYQIPIVRTRDITSNFVSGIIKYLNVQLAPRIVRHGVMIEVYGEGILILGESG
                     VGKSETAMELVKRGHRLIADDVVEIRRVSDTTLLGTAPDIIRHFVEIRGIGILDVKNL
                     YGMGSVKVQETVNLIIHLELWDKNKSYDRLGIDDEYTDILGIKIPSLTIPVRPGRNLA
                     IIVELAAMNNRQKSMGYNAAKVLNERINSRMMGEISRRKKER"
     gene            21334..22260
                     /gene="murB"
                     /locus_tag="PK733_03535"
     CDS             21334..22260
                     /gene="murB"
                     /locus_tag="PK733_03535"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOJ09647.1"
                     /translation="MNDICNIVRIADKIIEIAGKENIKFNEPLKYHTSFKIGGPADIF
                     VMPDGISAFVELLKLCKIQNIPYYIIGNGTNLIVRDKGIRGVVIKTGDKLGKCSVNNN
                     IIEAESGILLSVLSKIACNYELSGLEFASGIPGTLGGAVIMNAGAYGGEMRDVVIKTQ
                     YLDSEGDIKVLEGQQHLFGYRTSLIQKENGVILKSWLRLKRGEKRLIEEEMQVLKDKR
                     EKSQPLDMPSAGSVFKRPKGHYTGPLIEQCGLKGYSIGGAQVSNKHCGFIVNTGDAAA
                     EDVIGLIKFVQDTVKKQFGVELETEVKIIGEE"
     gene            22267..23130
                     /gene="rapZ"
                     /locus_tag="PK733_03540"
     CDS             22267..23130
                     /gene="rapZ"
                     /locus_tag="PK733_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOJ09648.1"
                     /translation="MEFLVITGMSGAGKSLVAKYMEDLGYFCVDNLPPALIPKFAEII
                     SQNNGKVQKIALVIDVRGGELLNDIFPGLNALKQLGFTYKILFLEASNMVLVKRYKES
                     RRSHPLAPSGRLIDGINEERKVLQKIRDNADHVMDTSNLLPSQLKEEMHNMFFEGREF
                     KGIIINIISFGFKYGVPIECDLVFDVRFIPNPFYVKALKKLSGKNEAVRDFVIGNELT
                     KIFLDKINNLLDFLIPNYIKEGKSQLIIGIGCTGGRHRSVAIAESIYRYLVEKQNSVI
                     IDHRDIDKDNR"
     gene            23196..24341
                     /locus_tag="PK733_03545"
     CDS             23196..24341
                     /locus_tag="PK733_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOJ09649.1"
                     /translation="MIGEIFNKLFYGILGMKSGTLCKQKNCRGLTNGRKSLSKGPKIV
                     TIGGGTGLSTMLRGLKLYTSNLTAVVTVADDGGGSGILRKDLGIIPPGDIRNCILALA
                     DTEPLMEQLLQYRFEDGMLKGQSFGNLFLAAMNGISNGFEEAVKRMSDVLAVTGKVLP
                     VTLGNVHLCAQLENGHIITGESNIGKHNYFEKGTIEKVYLEPRNAAPLNDVLEAIYDA
                     DAIILGPGSLYTSVLPNLVVNGISEALKKSIATKIYVCNIMTQPGETDGYTVFDHIRA
                     IEKHTYNGIIDCCIVNTAQIPAKVRTKYLEEGAGMVKIDYDRLKETSTRIIYEELVSI
                     SNNFLRHDPAKLAETAIDLINQTAAPNKKSIIDYFFAKDSLRKTAKL"
     gene            24365..26350
                     /locus_tag="PK733_03550"
     CDS             24365..26350
                     /locus_tag="PK733_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOJ09650.1"
                     /translation="MEFGKGDWRSFERGIEKEWLLTNGIGGYASSTIIGANTRRYHGL
                     LVAALNPPVERHLALAKIDESITIGENRDYNLYSFKTSNGYIMKGYKHQQRFISSPLP
                     EFIYSVEDVIIEKKVTMVYGENTTAIVYRIINGCRPLKLRFAPLVNFRDYHHNSSRWH
                     MNFKTNVIENGAIITPYNLETKLSLVSDKGIFKTMDDCWFLNMYYPVEEERGLSAGED
                     HFIPGYFYVEAQPGEEKYVTIIATVEKEAKYIKDIKERNALTLIEREKGRIDRLVARA
                     GYKDEFRKSLVIAADQFIVSRKSTNSKTIIAGYPWFTDWGRDTMIALPGITLATGRFD
                     DAREILGTFAEYAKDGIIPNMFPDGGQPPAYNTVDASLWYFEAVNKFIEYTEDYAFVK
                     DKLYKTLKDIINSYINGTHLIKMDADCLLNAGNPYTQLTWMDAMAGGRAVTPRYGKPV
                     EINALWYNAFKIMSNLAAEFGNEKEKEYYENQADRIRASFVAKFWNEAGQCLYDVVNE
                     DFMDDKIRPNQIMAVSLSYPVVERDMAVKIVKKVLEELYTAYGIRSLSPKSKEYKGIY
                     IGNPYERDSAYHQGTAWSWLSGQFITAMINAYEGSSEILEKAKLLIWPFKDHIRDGCI
                     GSISEIFDGDDTGIPRGCFAQAWSVGEVLRAYSELVR"
     gene            26356..27264
                     /gene="whiA"
                     /locus_tag="PK733_03555"
     CDS             26356..27264
                     /gene="whiA"
                     /locus_tag="PK733_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOJ09651.1"
                     /translation="MSFSSVVKNELSRIHVDDKCCLKAELASIIAVGGQMEIREPEGL
                     KIGIITENAAFARRSYSLIKELYEVCPEVICRKSRKLKKHTVFILAIPDTSSIKKELD
                     IPVKDACCKRAYLRGAFLAGGSISDPEKTYHLEIATRYSTLARQLRDTINSYNLNAKI
                     IKRKGYYVVYLKEGDHIVDFLNIIGAHAALLELENIRILKDMRNNVNRIVNCETANLE
                     KTVNASVRQVRNIEYIRDNMGFGNLPENLREIAELRLKYSDASLKELGEMLNPPLGKS
                     GVNHRLRKLEEIAESMASQSGSQKCI"
     gene            27510..27875
                     /gene="spoIIAA"
                     /locus_tag="PK733_03560"
     CDS             27510..27875
                     /gene="spoIIAA"
                     /locus_tag="PK733_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HOJ09652.1"
                     /translation="MLQIKFSNRGNTLIVRLNGELDHHAADDVKRKIDNEMIKPACKN
                     IVFDLSKLIFMDSSGIGVIMGRYKNIYKTNGKIMIVCSNPHIVKLLEMSGIPKIIPIF
                     DNVDKAVKKALEVAEYDAK"
     gene            27883..28332
                     /gene="spoIIAB"
                     /locus_tag="PK733_03565"
     CDS             27883..28332
                     /gene="spoIIAB"
                     /locus_tag="PK733_03565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOJ09653.1"
                     /translation="MASYKGKPINEMTLIFPAKSCNESFARVVAASFVSQLDPTVEEL
                     ADVKTAVSEAVTNSIIHGYENQTGNIKMVCSLYQDSIEIEIIDEGNGIENIELAMQPL
                     YTSKPEMERSGMGFTVMETFMDKVNVESEVGKGTKVAMCKRFKSIGK"
     gene            28608..29351
                     /gene="sigF"
                     /locus_tag="PK733_03570"
     CDS             28608..29351
                     /gene="sigF"
                     /locus_tag="PK733_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HOJ09654.1"
                     /translation="MSNFSDVNTFEFIEKAKEGDKYAQSLLIEKNMGLVWNIVKRFQN
                     RGYEAEDLFQIGCIGLMKAIKKFDSSYGVKFSTYAVPMIIGEIKRFLRDDGLIKVSRS
                     LKETSSKVRIIKETMIKELGREPTVNEIAESLDISPEEIVMALEATYTPEYLYSNVAE
                     GDSSPILLIDRLDAESSLPETDLIDRITLRQVLNTLNPREKQIIVLRYFKEKTQTQIA
                     KMLGISQVQVSRLEKKILKELKNKINFKS"
     gene            29606..29821
                     /locus_tag="PK733_03575"
     CDS             29606..29821
                     /locus_tag="PK733_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09655.1"
                     /translation="MTEVLRKYKSVFIILGFTLIAAVIAFIIFLSGRNTTKIPLRGVF
                     VDETNFQKKYGEKVAEGVGTGLWLKKC"
     gene            29815..30258
                     /gene="spoVAC"
                     /locus_tag="PK733_03580"
     CDS             29815..30258
                     /gene="spoVAC"
                     /locus_tag="PK733_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOJ09656.1"
                     /translation="MLKDLKPAEYRNYVESKSPKSKLLTNVFRAFLIGGLICVVGQFI
                     TNALKSRGYDSDTVGALTSVIMIFIGALLTGLNIYDEIGKYGGAGSAVPITGFANSIV
                     SPALEFKSEGYIMGVGAKMFVVAGPVLVYGISASIVAGILYYLLK"
     gene            30270..31289
                     /gene="spoVAD"
                     /locus_tag="PK733_03585"
     CDS             30270..31289
                     /gene="spoVAD"
                     /locus_tag="PK733_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOJ09657.1"
                     /translation="MADKRLGKQTVKLQNPPSIVSTACIVGPKEGAGPLGEYFDMVID
                     DELWGEKTWEKAESKIVKETYLRILNKASATPFDVNYIVAGDLLNQCIASTFGLRGSS
                     VSHFGIYGACSTMSESMNIGAMLIDGGFADNVLCITSSHFCSAEKQFRFPLELGNQRP
                     PTSQWTVIGSGGALLSNKGAGPYITHVTTGKIVDMGITDANNMGGAMAPAAADTILCH
                     FKDTGFAPEYYDLIVTGDLGIIGKTICEEMVNEQGFSISDRFNDCGLMIFDKKRQDVH
                     AGASGCGCSAVVFAGYIYKEIMKGTFRKVLLVSTGALLSPTSSQQNESIPSIAHAVTI
                     SNTLE"
     gene            31310..>31433
                     /locus_tag="PK733_03590"
     CDS             31310..>31433
                     /locus_tag="PK733_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HOJ09658.1"
                     /translation="MFWNYLYAFITGGIICVVGQLLIDKTKMTSARILVLFVTTG"
BASE COUNT        10902 a         4758 c         6725 g         9048 t
ORIGIN      
        1 gcagatttaa ggataaatgc agcaatactg catataacgc agaaatgatt ctacatggaa
       61 taaattaaaa gggggtattt aagtgaattc aaccattaca aaactctctt caatcaaggg
      121 gagaacatcg agttactggg gtgaaagaat cgtatcccag ttggagtatg gggttaaact
      181 atcccgcgta aatgacagaa gttatgatga actgattgga actgcagtaa aatttcttga
      241 caacaagctt gcggaggagg gagttattac aaaacaagtc gctcttgaaa ctgaaaaaat
      301 gcttgcaggc atgtcagaag ctgcaaaaaa acttaaagtt atttgtgctg ctcatgcaca
      361 tattgatatg aactggatgt ggggttatgc ggagacagtt gctgttactc tggatacctt
      421 caggacaatg ctgaatctaa tgaaggaata tccggaattt acattttctc aatctcaagc
      481 atctgtttat aagattattg aggaacataa tcctgatatg cttgaggaaa ttaagaaaag
      541 agtaaaagaa ggccgttggg aggtaactgc atctacatgg gttgagaccg ataaaaatat
      601 gcccaatgga gaaagtctgg caagacatat actttacaca aaaaaatatc tttcagaatt
      661 gttagatatt tgtccggatt ctctaaaaat agattttgag ccggatacct ttggacacaa
      721 tctaaatgtg ccggaaattc ttaataatgg aggagtaaag tactattatc attgcagagg
      781 gtatgataag cgtgttgttt acaggtggaa gtctccttcg ggaaacagta ttttagtgta
      841 cagggatcct ttctggtata atgccgaaat caacccttcc cttgcactgt atgtacctga
      901 attctgcgca aaacatggta tagatacaat gtttaaagtt tacggagtgg gggatcatgg
      961 agggggtccc accagaaggg atattgatag aataatagat atggcatcct ggcctgtatt
     1021 cccacaaata aagtttggta cttttaccca atactttgct attctcgata caattattga
     1081 taaattgcct gtagaggata aggaactgaa tttcatattc ccaggttgct atacatctca
     1141 atcaaggatt aagatgtcaa acaggattgg tgaagcaaag ttaaatgaag cagaagcatt
     1201 tagcacaata tctgctgctt ttgcgggagg gaaatatagc agcaaggccc ttgaagaagc
     1261 atggaggaaa gtgcttttta atcatttcca cgatatcctt ccggggtcag gtgtaataga
     1321 taccagagag tacgctatgg gcaaattcca ggaagttttg gccacagcaa atactgaaat
     1381 ttataatgcc tttagaaata tagtatctca agcggataca actggccttc tgaccttcga
     1441 agaagatata acatatacga tgtccgaagg tgcaggtgta ggctttgcca taaaggattt
     1501 tggtgttcca cagacagaaa gaggcaaagg gaaaggcaga atattccact ttttcaattc
     1561 ttcagcctat gaaagaatag aacccgtaga aattgttgtt tgggattggc cgggagataa
     1621 aaacagaatg ttcattaaag acgcttccgg taatcctgca aggtaccagc tgctgaaaaa
     1681 tgataaatat cagttttttg gggagtctta ttggggtcat acatatatac gtgttcttgt
     1741 tgatgtaaaa gtaccagcat atggttatac tacctataca ttgattgaga aggaaatgga
     1801 tgatcttccc gtagcactgg cgaaagaacc gagagttgaa gaagagaatg cctatattct
     1861 tgaaaatgat catattaaag taacatttga tacaagaaat gcatctatta tatctatagt
     1921 agataaaaag agcggggagg aaatggtttc tccaaataga cctgcaggaa atttcagact
     1981 tattgaagag gatgacagca aaggaatgac atcatgggta gttggcaggt atatgcagat
     2041 atttaattta aatgaaatga agaatgtaaa aatcagaagt tattatgggg aaaaatccgc
     2101 cttgagacag tggataagct acagtatcga attcagagca tcaaaactta atgtaacagt
     2161 atctcttgat tacaatagtt caagacttaa ctatgtagta gagtgcgatt ggcaggaaaa
     2221 acctgaaaag gggaaatttg ttccgcaact aaatttctac atgcctttga attatgaatg
     2281 cagaagatat aaatataata tagcttttgg tactatagaa agagaagcgt tgaacatgga
     2341 tgtgcctgct aacagctgga tggcgggaat atctgcatgt aactcaaata attacagcag
     2401 attggattgc aaagatggat gtggtaagat gcttatgctt attacagata caaaatatgg
     2461 cttcagagga tatgagaatt cacttgcagt aagccttatc agaagctctt acgacccgga
     2521 tccataccct gaatcaggca ttcacaagtt taaatttgct gtagatgtgg tggatatatc
     2581 ttttgatgaa aatgctatta acaatacatt aatacggaaa gcatatgaat ataatcatcc
     2641 tgtacatttt atttcaggaa caatacacga aggaacattt cctattgaaa atagttttat
     2701 ttcccttgag tccggaactg ttgccatatc tgctgttaaa atgcccgagg tcaataggat
     2761 tgacatacag caaaaagaaa gcagaagtga aggttcctgt agttgctgcg agccttgtgg
     2821 ggaaattatt tcatggaaaa gcgcaattct aagggcatat gaaactgaag ggagaaagac
     2881 ccgtgcagtt gttaagtttg cagaaaaagt cgcggaggca ttctttgtag atattaatga
     2941 aaagccttta gagtacaagg ttaaagataa taattcaggg aatgaatgct gtatagataa
     3001 ttgcaatggt gtagatattg acggagttaa tattgcattt gatgttaatg gatacagtgt
     3061 ggcaagtgta tacgtgaaat ttgaaacaga agaagcagaa gaataatctg aaaaaatcat
     3121 tgcataatat aaatataatt gctataatac tttataacaa attattgtta taaatattac
     3181 caaaggctat gagggagaaa agtaaacaga tacatcaaaa tgctttattc agagagaagg
     3241 taccatggct gcaagtatct tcataaagga agctgttgaa gttcactccg gagctgcatc
     3301 ccgaaatgta tcgaaccaga gtaggcgatg acggaacaga ccgttaatgc tgaagaggaa
     3361 ttatttgttc cttaaacatg agcgcctgtt aataatatct tgaaacaatg aaatcctgaa
     3421 cctttgaatc ataggtattt tagcaggaat atgggtggta ccgcgaagac ataccttcgt
     3481 cccttgtgga tggaggtttt ttatttttta aaaggggata tttcgccata tagaagttag
     3541 gagtgtgtcc ccttacatta aaaggagatg gatggaattg agagaacaat taatcaatat
     3601 tagagaaaaa gcaaaagaag ttatttcctc agtccgctcc atgcaggagt tggataacgt
     3661 gagagtgaaa tttttaggta aaaaaggaga gttgacccct atactcagag gaatgggttc
     3721 actgtctccg gaagaaagac ctgttatggg gcagcttgta aacgaagtca gaacatcaat
     3781 tgaggagctg cttgagcatg caaaaaatga ccttgcacaa aaagaaaaat cattaaaatt
     3841 gcaacttgag gttattgatg taacaatacc gggaaaaaga aaaaatcttg gaagcaaaca
     3901 cccgttaagc attgttatgg acgaaataaa ggatgtattt attgggatgg gatatgaaat
     3961 tgccgagggc cctgaaattg agctggacta ttataatttt gaagcattga atacgccccc
     4021 gggccatccg gcgagagatg tgcaggatac tttttatata aatgataata ttttactgag
     4081 aacacagact tcgcctgtac aggttagagt tatggagaag cataagcccc ctataaaaat
     4141 agtttgtccc ggcagagtat atcgttcaga tgcggttgat gcaactcact caccaatctt
     4201 ccatcaaatg gaaggacttg taatagataa aggtgtaact atgggagatc taataggaac
     4261 tttgcaggtt tttgcacgca gcctttttgg cgagaacaca aaaattcgtt taagaccgca
     4321 tcacttccca tttaccgaac cgagtgccga agtggacgtt tcttgttgga cttgcggtgg
     4381 aacaggctgc agagtatgta agaacagtgg atggatagaa atccttggag ccggaatggt
     4441 gcatccgaga gtacttgaaa tgagcggcat agatcctgaa atatatagtg gttttgcttt
     4501 tggtattggt attgaaagaa cagctatggg tcgattcaat atagatgatt tgagattgct
     4561 gtatgaaaat gacttgagat ttttgaagca gttttagcag tttgaagtaa ttttagcaaa
     4621 gtaagttagc tctctgactg aaaggcattt gccaagttaa atataaaatc cgagatagga
     4681 gttgaaggtt tatgaacgtg ccaataaagt ggcttaaaga atatgttgat ataaatgtta
     4741 gcgtggaaga gttgtgtgac gcattgacca tgtccggttc taaggtagag aatattgaaa
     4801 aacagggaga agaaatatct aaagtagttg tagggaaaat tctctcaata gaaaagcacc
     4861 ctgatgcaga taaactgctg gtaacaaaaa ttgatactgg tgatgaaaca atacaggtag
     4921 taacaggagc ccataatata tcagaaggtg actatgtgcc ggttgcttta catggttcta
     4981 ccctgcctgg aggcataaaa ataaagaaag gaaaactcag aggggttgaa tcaaatggca
     5041 tgctatgctc aattcaggaa ttggagctta caaaaggtga ttatcctgaa gcagatgaag
     5101 atggaatatt tatactccgc aatatggaaa acttgaaact tgggcaggat attaaggagc
     5161 ttgttggatt agatgataca gtagtggaat ttgagataac ttccaacaga cctgattgct
     5221 tgagtgtttt aggaattgcc agggaaaccg ctgctacttt aaaaaccaat ttaaaaaaac
     5281 ctgtgattaa tgtcaaagag gaagaaaatg ataaggcata tgtctatgct caggttgaaa
     5341 taagaaatcc tgaactatgc tcaagatatg ctgcgagagt ggtaaaagat gtaagaatcg
     5401 gcccatcgcc ggaatggatg agaaaaagat tgagagctgc aggagttagg ccaattaata
     5461 atatagtcga cataacaaac tatgtaatgc ttgagcttgg ccagccaatg catgcttttg
     5521 atttgagtta tataaaagac agtaaaattg ttgtaagaag agcagaagat ggagaagtaa
     5581 tgaaaaccct ggatggacag gaaaggaagc tggattcttc catgcttgtc attgctgata
     5641 gtgaaaaacc tatagctgtt gcaggggtta tgggaggaga aaattcagaa gtaaatccaa
     5701 acacaagaat tatacttctg gaatcagcta attttaacgg aatttctgta cgtctggcag
     5761 ccaaaaaact tggaatgagg acagaagcat cagcacgttt tgaaaaaggc cttgatgtag
     5821 aaaatgtgat aacagccgtt aatagagctg ctcagttaat cgaggaaaca ggagcgggaa
     5881 aagtatgctg gggaataatt gattgcttcc cgggaaagaa aaataaaaga gttcttaagc
     5941 taaggccgga aaaaataaat gcatttcttg ggacatctat cagtttggat actatgatag
     6001 aaatacttga atccgtagag attagagttg ataaggacac catgacagtt attatacctg
     6061 aattcagaca ggatcttgaa agagaagcag atcttgcaga ggaaatagca agattataca
     6121 attacaacaa tatagaagcg actcttttaa aggggaaagc tgccacaatt ggcaaaaaaa
     6181 ctactaaaca aaaaatcgag gatataataa agaataccat gatgtcctgt ggtttgtcag
     6241 agatatgtac ctactccttt accagtccaa gggtttttga cagaatcaag gcagcttcga
     6301 atagtgaact aagaaaagct attcctatct caaacccctt gggagaagat tacagtataa
     6361 tgcgcacaac aactgtaccg gaaatgttgg gggttattag cagaaattat agcagaagaa
     6421 ttgaaaaagc aggcttcttt gagctggctt ttgtatacct tccggaggaa ttacctctta
     6481 aaaagctacc tttggaaaaa gaagtattga ctctaggtat gtatggagat accgattttt
     6541 atgatattaa gggtatagtg gaggaactgc tttatacgct gaaaataaaa gattataagg
     6601 ttgtcccgga aagtggaaac gataccttcc acccaggcag aactgctaaa ataattattg
     6661 acggtgagcc tgcaggaata atcggtgaga ttcatcctga tgtatccgaa gagtttggtg
     6721 ctcccgaaag aacatatata ggtatgattt atgtggaacc attaataaga aaaactgtaa
     6781 tggaagttca atacaagcag cttccaaagc accctgcagt gacaagagat atagccatac
     6841 tactggaaga agaaataatg gctggagaca ttgaacaggt tataaaggaa aacggcggga
     6901 aaatatttga gagcattgag ctttttgatg tttataaagg aaaacaggta cctgaaggta
     6961 tgaagagtat ggcttattca ataactttca gagcagatga cagaactctt acagacgatg
     7021 aagtaaacaa gggcatggag aaaatcgtgt cagctttgtc ggataagttt aacgcaaagt
     7081 tgagagaata atattagccg ttcctgttgt atttggtgcc cggcaccaaa tacaacagga
     7141 atatttacag aaggagggtg aattttttta tggataaaat aagttttgac tactcgaagg
     7201 cggataaatt agttgcacaa catgaggttc aatattttaa agactttgta aaacatgctc
     7261 atgaaatgat ccatagcaaa accggagctg gaagtgattt tcttgggtgg gttgacctac
     7321 cgttaaaata cgaccaggaa gagtttgtca gaatcaaaaa ggcagctgag aaaataagat
     7381 ccaattcgga tgcactggtt gtaataggaa tcggaggctc atatctcgga gccagagcgg
     7441 taattgatgc attatcgcat tccttttata acttgcttga taaaaataaa agggaaacac
     7501 ctgagatata ttttgcaggt aacaatataa gccccaaata tctttcggat ttactggata
     7561 tacttgatgg aaaagatgtt tcagttaatg ttgtttcaaa atccggtacc actactgaac
     7621 cggcgattgc attcagagtg tttaaagaat ttatggaaaa gaagtacggc aggaaaggtg
     7681 cagcagaaag aatatatgcc actactgaca aatcaaaggg cgcaatgaga aagcttgctg
     7741 aagaagaaaa atatgaaact tttattattc ccgatgacgt aggaggaaga tattcggtcc
     7801 ttactgcagt agggttgctt cctatagcag taagcggact tgatattgat gccctcatgg
     7861 atggggcagc agctgcctgc aggcaatata ataatgataa tctaaatgag aatgaatgtt
     7921 accaatatgc tgcagttcgt aatgccctat atagaaaagg caggacaaca gagattatgg
     7981 taaactatga gccgtcactt cattatttca cagagtggtg gaaacagctt tatggtgaaa
     8041 gtgaagggaa agaccagaaa gggattttcc ctgcaggtgt agatttttca acggatcttc
     8101 attccatggg tcagtatatt caggatgggc tgagaaatat atatgaaact gtattaaata
     8161 taaacaaatc aggtatcgat ataaccataa aagaagacaa ggataatgtt gacgggttga
     8221 atttccttgc aggaaagacc atggattttg taaacaaaaa agccatgcag ggtactatcc
     8281 tagctcacac agatggagat gtacccaacc ttgttatcac tatacctgag ctcaatacct
     8341 ggtatctggg aaatattatt tatttctttg aaaaggcttg cggtataagc ggatatctcc
     8401 ttggggtaaa tccttttaac cagccggggg tagaagcata caagaaaaat atgtttgctc
     8461 ttctgggcaa gcccggatat gaagatgagc aaaagaggct ggaatgcatg ctgtaaaaat
     8521 gctaagatac ttaattgatc tcataaaatt gtctgataga aaactaagaa acaattttct
     8581 tcacttaaat tatatgtacc catttcatta gggcttagaa aagaggaaac ttgaaatcat
     8641 ttcaagtttc ctctttatat ttttattata aaagtgtgta ttacttatct actggtaatc
     8701 ttcgaaatac tcctaatatt tcctgagtat tcacttgtgt tattattact gctgtaaaca
     8761 ttagaacaca accaattatc tctctgggac tcattatttc gttaagaaat acagctccac
     8821 ttattgcagc gaacacagat tccatactta atatgattgc tgcaactgta ggatttgtgt
     8881 acctttgacc aataatctga agtgtatatg ctacacctac caccattata gctgtgtaaa
     8941 gtattggacc tgcgcaatct acaatagcat gaatgtcagg attcccaaat ataacagctg
     9001 ctatagctga aagtatacct gcagttatga actgcacaag tgatagctta agtgcttcta
     9061 ctttatcagc ataggcgtct actactatga tttgcagtgc ccagaaaatt gtgccaatta
     9121 gtacaataac atctcccttt tgcatagaca ttccttcctt aatgcaaagc aagtacaatc
     9181 ctgctatagc aagtgctatg cctgcccaag tcggaagatc tgtcttctta ccagtaaata
     9241 ttcccaacaa aggaaccagc acaatataaa gagttgttat aaaacctgct ttcccagctg
     9301 ttgtatatac aataccccac tgttgaaagg atgctccaag aaacaaggtt ccgccgcaca
     9361 gtaatccacc aataatcaag tctttatttt taaactgagg tttattggcc tctgcaaaaa
     9421 atatattctt tttacttctg tttaatgcgt caaagataag tattgcggga atcaatacta
     9481 ctgcaccgag cagaaatctt gatgcaccaa aagtaaatgg atctatatga tccattccca
     9541 gcttttgcga tgcgaatgaa gttccccata taattgctgt tattaaaaga attaagtttc
     9601 ctttaaattg cttcaattta acttaccttt ctgctgaata ataattgtac acaagaactg
     9661 tccccggcgc actaagatat taatatccaa gcatggctgg gaaaagtatg cttataagag
     9721 ttataataat tgcagagatt attcctccta tggcaacaca taaaaatgat tttttgaaat
     9781 ccagacccat aaatgcagcg acagcacttc cagtccagag ccctgtagtt ggaagaggga
     9841 tagctacaaa gattatcaac cccagttcct tatacctttc tacttttacc gagcccttac
     9901 gcactttatt ttcaataaaa tcattcaact tgcttaaaaa tttgaacttc ttcatccact
     9961 caaatatgac attgaataaa agtaaaataa agggtacggg aaggagactt ccaagaaaac
    10021 ttgcagcaaa tactactata gggcttaatt tatatgcaag gatgcctaca ggtatggcac
    10081 ctctctgctc gatcaggggt actgcagata taaaaatgat ttttaacaag tttacaatat
    10141 attggctcat atcactcttc ctttgttata tattgcttga gccaatggaa cttttataaa
    10201 ttgatatatg cttccagtga ctcaactcta cttactgttt gaagcaaagt aataaatatt
    10261 agtatagcat atggcagtgg ttggctgcaa ttaaaaaaat tatggcaaat aaaacacagt
    10321 aatttcaggc cttgatagaa acctcaatgg gaagcaaacg tttcccagac ctcggtttat
    10381 atacaaattt ataccattca gtttgtgaag tcctctgtta atacctatcc tgcacaattt
    10441 ttcctttcta agcaaattaa attcaaggtg aaatggcatc catatctgtc ctccatgaaa
    10501 atggccgcaa aaaaggtagt ctactttacc ttttggtatt tgaaagatta tatcgggatt
    10561 gtgagaaaag gcaatattga caaaagcatc ctcggcgcag aaacttaaag ctttatttat
    10621 atcagggtgt cctgcgttaa catcgtcaaa tccaattata ttatatttta cagaattttt
    10681 cgaataactg actgctctat tatataaaac agtaactcca agactttcaa tggattgtat
    10741 aaaatcctca atattatgag attgatattt ggaatgacca tgaggcttgc aatgaaactg
    10801 cctgtgatat tttccgcaat tccgatcaaa cgctttataa tcatggttgc ccatgcacag
    10861 atagattaca gtgtcacttt ttattgtcct tatggtttca agaaaatgta ggaatgcatc
    10921 aatttggtct tggttttcaa tataatctcc tgtcatgatt ataatatccg gcttttcacg
    10981 agaaataaca ttctttactt tttgagcaga aaccttcaaa tacttcatat gtatatcgga
    11041 taactgtata attttaagac ctttattgct taaagtgaat ttgactctgt caactttaag
    11101 ccatcctgct tcaaatctca tatataaaag cattattagt ataaataata aaaatgagta
    11161 tatataaatc attagtttcc tccaaactaa ttgcttcaat ctctattggc cgatgtacct
    11221 atagtgaggt ataacagatt taataaattt actgaattgt attatatcag cagcattgaa
    11281 aaacatcaac tataaatgca cctttcaatt agaatggcaa ttgacatact ttagcagcaa
    11341 caatataata tggttagaat agcagataag gagaatactg atgaacagta gttttggcga
    11401 caggtgcgaa atatgtgaag ttcataaaga taaaaccgaa tgtatgaaaa aatgcatgct
    11461 ggatgaatct aatgcagtaa ggctttctga agtctttaag gtcctgggtg accaaaccag
    11521 aataaaaatc ttatatgttt tatccaagtg cgaaatgtgt gtatgtgata ttgctgaagt
    11581 gcttgatatg tctcaatctg caatatcgca tcagctgagg gtactgagaa caatgaggct
    11641 tgtgaaatat agaaaggagg gtaaatcagc catttattcc cttgatgatg atcatatatt
    11701 gcagttattt atccagggta tggagcatat aaagcacagc tagtatttta tgttattagg
    11761 ctatttcttt ggtttcatac catatgcaaa gtaaatgcat gcaccaatag ctatgattat
    11821 gtctccgatg ctgaacacat ttgaaaatgg tatatatgaa gggatggcaa aaatatctcc
    11881 aagccatggc aggagagtat catcagctat ggcctttgaa ttgtttattg tttcaccacg
    11941 actgaggatt tccgtatacc tccctactga ggcactctca aatgattgta tagaagcggg
    12001 catatggcct ttgttgagaa ttattacaat tgcgtttaaa agtatgccgg tacctataaa
    12061 agcaacacca ggcagcttta tatttattgc tgtaaaagct aatattagta tatatgaaag
    12121 tatatgaaac gtgaccagaa ctgcagagtt ttgctgcagt gctttaaaaa tagaaatatc
    12181 tgaaaatatc agtatttgaa taaggaaacc tgaaatcacc agccatatat gcttaaaagg
    12241 attttgagca aggaatttaa gtcttccacc tgaaatatat cctattagta ttcctattat
    12301 aacagcataa ataatgaaca tttagaatta tctccttatc tcacctttat cattaatatt
    12361 attacattta tcatttgttt tatcatcggt aacggtatat tcattatccg atttatcatc
    12421 aatactggta tgattattat ccatatcaaa ataaccataa tctcttttta atacttctat
    12481 aaaggcttta acaactacag ggtcgaactg cgcacccgaa tatttcagca gctctttaat
    12541 ggctgtcgat tgtggaagag cttttcgata tgggcgatcg gatgtcattg cgtcataaca
    12601 atctgcaaca gcaaggatac gtgcccccag gggaatgctc ctccctgaaa ggttttccgg
    12661 atatccggtt ccatcaatct tttcatgatg atatagagca tacttggcac catttttata
    12721 gggcttcagg ttttttaata gacggtaggc tacttccgga tgctttttta tgttgtcata
    12781 ctcttcagtt gtcaactttc catctttcct cagaatgcta tcttctattg ctattttgcc
    12841 tacatcgtga acacgtcctg cagtttccac ttcattgatt gcatccgagg aaaggtgaag
    12901 ctcttgagcg attttaccag aatattcagc cacccgcgat gaatgagaat aggtatagct
    12961 atcacgttca tctataatat cagccagcac attaagtgtt tcaatagttt ccatatgtag
    13021 actgtagtat ctgcgtatgg attgatctgc cataataaga gggggtatga ggatcaaagc
    13081 tttatatggg tggctttcct ggtaaagcaa ggatgcagct atgcttatgg gggttatgct
    13141 gaagtaaaac gcagccatga ttttataatc attgaaaaag acatcaatga atttgttgcc
    13201 gtaaaagaga gaaataacga tggaaataaa caatgtattc ataaaataat atactataac
    13261 cgatgctatt atagcaggta agtctttaat aatatccagt ggtatatcat ttggtgacat
    13321 ctttagtaaa ttgaaaacaa agcttgtaac taataaggaa agaccgtact gacctgcatt
    13381 gaacattgcc ttataccatt ccttcttaga aatcagttca gctattattg tagcaacaat
    13441 aacaatccat actgcctccc aggtattcag aagaattatc atcgccaact ggacggaaaa
    13501 tgacagagtc atatccattc ttaaataaaa gatgcttctg aaaggtttta aatctgcagc
    13561 taactcaaga ataatccaaa acaatatgtg aaaccagcct ttaaaattta tatgtggtgt
    13621 agagtaggaa attagtggaa atacactaaa accaatcaaa taaacgtaaa agtatttaag
    13681 attaaacctt tttaaaacat tttttatttg cagaaaatga ttacgcattt atgtttactc
    13741 ctatcaccgc aataaaacta tagtattagt aacaatggca gcattcaatt gtcacaaagc
    13801 aaaatttaat tgatatattt catccgactt acaagtattg caactttagt gctgaagtcc
    13861 cactaactaa attataatga tgttttgaat aaaatcaaca agtaaaagaa aaaatagcct
    13921 tgttttcaag actattttct atttattata tatatgtata aatttttatg ctaaaatata
    13981 aatgcttatt tgtaactctg cacgactaaa attgatagtt taaccccatt tccagcctgc
    14041 tactcctgct aacactaatg taatgacacc tgctaattga agaataaccg gaacaattct
    14101 accccatttc ttcatattga tttcctcctt tcaaaatatg taataataaa aataatttta
    14161 tgttggatat aatttgaact taatttatta tttaagcaaa taataacata taacgcagat
    14221 tatgtcaagc actatttaaa aaaacaataa aaaaacttat ggaaaccttc aatattttgt
    14281 tacatatgta caaaaacatg gacgtatctt ctatatttaa aggaaaatgt ggttaaaatc
    14341 tgctttaatg tataatgctt ccttatgtaa gtgggtaaca ttccagcacc tcactgcata
    14401 atatgaaatg agattgagtt aatttgctga ttgcaggagg gaatgataat ggaaaacggt
    14461 aaaggatttt tgggtagatt atttggcaat gatatgattc tatggtttat tattttgttt
    14521 ctattgttgt tttggtgcac caattgtttt agagcggaca accgtgaaaa tgatgttgat
    14581 ttaggtctgg cacatgagta aattgttaag cattacacct ataagttaac aaagcagcaa
    14641 tattaatcct ttcaatactg gttaatactg taaaaaacaa tatggtttgc agagcctgta
    14701 cataaatgta gttaacatca agaatatttg aataagagtc taaaatacag gctctttttt
    14761 ttagtgcttt cagtttacat aaacaaccaa aatcaataga aactgcaaga aatttcaata
    14821 tgaaataata cacggtcata atctttaaaa actggtataa tcaattataa atagtgtaaa
    14881 acaatgcaat tttgccattt catttatatt caattctgct ataatttaaa agaagtatta
    14941 acaattcaat tctgtgggaa gaagactccg ggatttacgg aaactacatg agtcttgaca
    15001 agacagctac attttccggc tgttgaaaat caggaattgg acattaatta ctgagaattt
    15061 tggtataaaa ataagcttta tttttgtatt aaaaacatgt aaaccaaaaa ttcagcctgg
    15121 tgcgaaccaa ttaattgact ttagtatata ctgtaccggg caagtgtacg ggataatgga
    15181 ttttaaggtt cagaaatcag gcccacagga ggatgattaa attggaaatg gaagcagttg
    15241 accctttacc tggtggtcac cgctacaatg tagggttgac ctataatctt aagaagggtc
    15301 tcaaatccga tgtagaggat attgaggccg agttggatag ttttgataca gtaaatgcta
    15361 taaaagaagc attagagaaa ctaaagtgta atgttacact tcttgaagct gatgaaagcg
    15421 ttataccaaa tctgcttaaa gccagacctg atatggtttt taatattgct gaaggcttaa
    15481 acggaagagg tagagaagcc cacatacctg ccttgttgaa ttttcttgga atacctttta
    15541 caggttctga tgagactact ctatgtattg cccttgataa gaccttaaca aaaagaatat
    15601 tatcaacata tgggatcaat acccctatgt attgccttat taaaaatcca tctgaaaagc
    15661 tgccggtaaa tatgaaattt ccagtaatag tcaaaccaaa tgctgaaggt tcaagcaagg
    15721 gtatttcaga tatggcaata gttaaaaaca tggaacagct aaaaggttta attgagaaaa
    15781 attatgaaat gtataaacag ccaatgctgg ttgaagagta tattaaagga agggaattca
    15841 cagtagctgt tattggaaac agcgatgatg caaaagtatt tcctcctatg gaaataaggt
    15901 ttaaaaggca ggaggaacaa tatagcattt acagttacaa tgtgaagaaa aattataagg
    15961 aatatataga atatatttgt ccacccatga tagatgaaaa tatactgcag aaaatggtag
    16021 aaacggcacg aaaagtatat gaatgcctgg attgtagaga tttctcaagg atagacttcc
    16081 gagtttccga tgagggggaa gtgcatttta tcgagattaa tcctttgccg ggtcttgcac
    16141 cgggatacag tgattttcca atgataatgg aatttaacgg ggaaagctat gacaatacca
    16201 tcaaaatggt gctgaacagt gctcttagaa gatatggtat gaatactgta aaatgatggg
    16261 gaggatgaat atgagagatt ataagactgt tgatatatgg aaagatgttg atatagaaca
    16321 atgggaagac tggaagtggc agttaaagaa caggattgta actgctgaac aactgggcaa
    16381 ggtaataagt ttatctgaaa aggaaatgga tgatataaat ctttgcctaa aaatgttcag
    16441 aatggctata actccatact actccatgct tattaaccct gacgatgata attgtccaat
    16501 aaaagctcag gcgatcccat ctataaagga attaaatata agatcggatg atttatcgga
    16561 ccctctgggt gaagaatttt attctcctgt agaaaatatt gtacacaggt atccggatag
    16621 agtgttgttt cttcttacgc ataaatgttc catgtactgc aggcattgta ccagaaggcg
    16681 tgtagtagga gaagaagatt tttccgtaaa tgaagaaacc cttgataaag cagttgatta
    16741 tattaaaagt aacagtaaaa tcagggacgt ccttttatcg ggtggtgacc catttattat
    16801 gagtgatagc tatcttgagc atataataca gcgattgagg gagatacctc acgttgaaat
    16861 aatcagaata ggcacgcgta cacctgttgt tttacctatg agaattacag atagcctaat
    16921 aaatatgctt aagaagtatc atcctatatg gataaatact cacttcaacc accctatgga
    16981 aattacggaa gcatcagtta aggcatgcgc aaaaattgtc gatgcaggta tacccctggg
    17041 caatcagagt gtgcttttaa aaggtgtgaa cgataatgtg gagactctga aagaattgct
    17101 ccttaaactt gtaaaagcca gggtaaggcc ttactacctt tatcagtgcg atattgctca
    17161 gggaattgga cactttagaa caaaagtgga ggaaggcatg gaaatcatga agaatttaag
    17221 gggctatata tcaggatatg cagttccaac atttgttatt gatgctccaa acggaggagg
    17281 gaaaattccc attaatccgg aatatgtagt ttctatggat gatgagagag tagtaatgag
    17341 gaattataaa ggtgacatat atttctatcc taacgcaggc taaagtgtga agaggtcaaa
    17401 ttgagcgaat acaaatatac caatgttatt gaacaggctg gaaaagtttt ttccagcctg
    17461 tttcagattt aaactttggg tgcatttaat ttaagtgaag aaaatattgg taagcagcgt
    17521 aacgacagct cctggtaaat atttcttcac ttttagtaaa tacacctttt aaacattcat
    17581 aatgttgacg aaattaaata taaaatatag aattaagatg atgtatataa tgcaaaattt
    17641 taaacaaatg catgagaaga tgcagaaatt attatagaaa taattatgaa agagtaaaaa
    17701 cttttacaaa gcatgagaac tgaattgatc aggtaaagtt agagaaaggg gtttatatat
    17761 gtttaagata gtaaaaaaag gggttctgaa tcctgctgta aaattattgg aggtagaagc
    17821 tccatatata gcaagaaaag ctgaacccgg acaatttatt atattaaggg ttaatgagga
    17881 aggagagagg atccctctta ctattgctga ttttaaccgt gaaaaaggta ctgtaactat
    17941 aatatttcag gaagtaggta agactactca attactgggg gctttgaatg aaggcgagtc
    18001 tatacttgat tttgcaggcc ctttaggcat gccgactcac tatggtgaag ttaaaaaagc
    18061 tgcagtaata ggcggaggcc tgggtactgc tatagcctat ccccaggcta aaaaactgca
    18121 cagtatggga gcagatgttg atgtaattgc tggtttcagg aataaggatt tgataatact
    18181 tgaagaagaa atgaaggctg taagcagtag aacatttatt acgacggatg atggttccaa
    18241 tggaaacaaa gggtttgtat ccgatgtttt aaaaaggctt atagaagaag gaaacaaata
    18301 cgatcttgtg gttgttatag gaccgttgat aatgatgaag gttgtaagcg gtattacaaa
    18361 agaatatgga attaaaacaa ttgtaagcat gaatcctatt atggtggatg gtacaggtat
    18421 gtgcggcgga tgtcgcgtta cagtaggagg aaaggttaag tttgcctgtg tagacgggcc
    18481 tgattttgat gggcacgaag ttgattttga tgaagctatg agacggcagt tgatgtataa
    18541 gaaagaagaa agacattcca tggagggaca cagatgcaga ttggagggta taaagaatgc
    18601 ctaatatgtc actaaaaaag atgcaaatgc cagaacagga gcctgaaaag agggttaaaa
    18661 atttcctgga agtggcgtta ggatatactg aagaaatggc agtggaagag gcaaaaagat
    18721 gccttaactg caaacataaa ccatgcacta agggctgccc tgtaaatgtt aagatacctg
    18781 aattcataac ccttgtagct gaaggaaagt ttgaagaagc gtatttaaaa attaatgaaa
    18841 caaacagctt acctgctata tgtggcagag tatgtccaca ggaatctcag tgcgaaaaat
    18901 tctgcgtaag aggaaaaaag ggtgagcctg taggaatagg cagacttgag agatttgttg
    18961 ctgattggta tatggcaaac agaaaaattg agcaaactca agttgagagg aaaaatagga
    19021 aagtagctgt tgttggttca ggccctgcag gtttgacctg cgcaggggat ttagctaaaa
    19081 tgggatatga tgtaacggtt tttgaggctt ttcatactcc aggaggagtt ctggtatatg
    19141 gaataccgga gttccgtctt ccaaaagaat tagttaataa ggaaatcgat accatacgaa
    19201 atctaggtgt tgacataaaa accaatatgg ttattggcag agtattaact ctggatgagc
    19261 ttaaaaaaga ggggtttgaa gctgtattta ttggaactgg agcaggtctc cccagtttta
    19321 tgggtatatc gggagagaac ttgaatggag tatactcggc aaatgaattc ttaacaagaa
    19381 ttaaccttat gaaggcttac aagttccctg attgtgatac tccggttaag gttggcagga
    19441 atgttgcagt agtaggaggc ggaaatgttg ccatggatgc tgcaagaagt gccaagcgcc
    19501 ttggagctga aaatgtttat atagtgtata gaagatctga agaggaaatg cctgcccggc
    19561 ttgaagaagt tcatcatgct aaagaagagg gtatcatatt caaactgctt acaaatccta
    19621 caaagattat cgggacagaa gatggatggg ttaaaggtat ggagtgtatc agtatggaat
    19681 tgggtgagcc tgatagttct ggaaggagaa gacctgtgcc caaagcaggt tctgagcata
    19741 tccttgacct tgaaacagtt attattgcta tagggcaaag tcctaaccca ttaataaaat
    19801 ctactacacc agatttggag acacaaaaat ggggtggaat tataactgat gaagaaacag
    19861 gagctaccag cattgaaggt gtttacgccg gcggtgatgc cgtaacagga gcggctactg
    19921 taatactggc catgggagca ggcaagaaag ctgcggcagc tatcgataaa tatttaagtg
    19981 agcgctaaat gttgatttca atgcagaaag tcatgttcca acaaaatgtc aatatatata
    20041 gcgaaaaaat atgagcctgg gggtaaaact atgtctgaag aatattcagt aacattaaaa
    20101 gaaatagttg atgattttca gcttgaaata atcagcggac ataacagaat ttatgatgta
    20161 aagattacaa cttccgatat taataggccg gggcttcaac taacaggcta catggaaaat
    20221 ttcggaagcg aacgtatgca gatagttggt atggttgaaa ccagctatat ttccggaatg
    20281 acgtctgatg aaaggtataa aaggcttgat gcatacttta aactggggtt cccatgtgtt
    20341 attgtaacca gaggtttgga tgtatttgag gaaatgatag aaatatccaa taaatatcaa
    20401 ataccaattg taaggaccag ggatataaca tctaattttg ttagtggtat tataaagtac
    20461 ctaaatgtac agcttgctcc cagaatagta agacatggag ttatgataga agtttatgga
    20521 gagggtattc tgattttggg tgagagcggt gtaggcaaaa gcgaaactgc catggaactg
    20581 gtaaagaggg gacacagact gattgcagat gatgttgtag aaattagaag agtatccgat
    20641 acaacattgt tgggtacagc tcccgatata ataaggcatt ttgttgaaat aagaggtata
    20701 ggaatacttg atgttaagaa tctatatggt atgggttctg taaaagttca ggaaaccgtc
    20761 aatcttatca ttcatctgga gttatgggat aaaaacaaaa gttacgacag actgggcatt
    20821 gacgatgagt atacagatat actgggaata aagattccgt cattgacaat acccgtaaga
    20881 cccggaagaa acctggcaat tattgttgaa ctggctgcta tgaataacag acagaagagt
    20941 atgggctata atgctgctaa agtactcaat gagaggataa attccaggat gatgggtgaa
    21001 atcagcagaa gaaaaaagga aagatagtaa aggaaataag gataaaacaa aaaactgtga
    21061 agtatgccgt gaacggattt atggcatact tcatataata atatactgca gggtacattt
    21121 aaaatttttg aagaaatata ttccagaagc tatcgttacg gtacttagag cattcactaa
    21181 tgcttcttac aggaatttct acaaaaataa aatatgcact ctaaactgca gccggtggtt
    21241 ctaattgcag ttggcagcaa ttaatagtag cagtagctat atattatagt cgcgagtata
    21301 tttaagaaga gtgaaaagga gtaaatggca ctaatgaatg atatatgcaa tattgtcaga
    21361 atagcagata aaattattga aatagcaggt aaagaaaata ttaagtttaa tgaaccttta
    21421 aaataccata catcattcaa aataggcggt cctgcagata tatttgttat gcctgatggt
    21481 atctctgctt ttgttgagct tttgaaacta tgtaaaatac agaatatacc ttactatatc
    21541 ataggaaatg gtacaaattt aattgtaagg gacaagggta taaggggcgt tgttattaag
    21601 actggagata aattgggcaa atgctctgta aataataata taattgaggc ggaatcagga
    21661 atattgcttt ccgtactttc aaaaattgct tgtaattatg aattgtcagg gctggagttt
    21721 gcatccggaa tacccggcac actgggaggg gctgtaataa tgaatgcggg agcctatggt
    21781 ggagagatga gggatgtagt aataaaaacc caatatcttg attccgaagg tgatattaaa
    21841 gtcctggaag gtcaacagca tctttttgga tacagaacaa gtcttatcca aaaagaaaat
    21901 ggagttattc tcaaatcttg gcttagactt aaaagaggag aaaagaggct tattgaggag
    21961 gagatgcagg ttttaaaaga taagagggaa aaatcacaac ctttggatat gcccagtgca
    22021 ggtagtgtat ttaaaagacc taaagggcat tatacgggac ctttaataga acaatgcggg
    22081 cttaaaggat attcaattgg tggagcacag gtttcaaata aacattgcgg ctttatcgtt
    22141 aatacagggg atgctgctgc tgaggatgta atagggctga ttaaatttgt ccaggatacg
    22201 gttaaaaaac aatttggagt ggaactggaa acagaagtta aaatcatagg agaagaatag
    22261 atacaaatgg aatttttagt aataacaggg atgtcagggg ctggtaaaag tttagttgca
    22321 aaatacatgg aagatttagg ctatttttgt gtcgataatt tacctccggc actaatacct
    22381 aaatttgctg aaatcatttc tcaaaataac ggaaaagttc agaagatagc actggtaatt
    22441 gatgtaagag gtggtgaact tctaaatgat attttcccgg gtctcaatgc attaaaacag
    22501 ttgggattta catataaaat attgttcctg gaagcatcaa atatggtttt ggttaaaaga
    22561 tacaaagaga gcaggagaag ccaccctttg gcaccttcag gaagactgat tgatggcatt
    22621 aacgaggaaa gaaaagttct tcagaaaata agagacaatg cagatcatgt aatggatact
    22681 tcaaatcttt tgccttctca gctaaaagaa gagatgcata acatgttttt tgaaggcagg
    22741 gagtttaaag gaattattat taatattata tcctttggat ttaaatatgg tgtcccaata
    22801 gaatgcgacc tggtttttga tgtaaggttt ataccaaatc cattttatgt taaagccttg
    22861 aaaaagcttt cagggaaaaa tgaagcagtg cgggactttg ttattggaaa tgaactaacg
    22921 aagatatttc ttgataaaat taataattta ctggacttcc ttataccaaa ttacataaaa
    22981 gaaggaaaat cacagctgat aatcggaatt ggctgtacag gcggaagaca tcgttcagtg
    23041 gctattgcgg aatcaatata ccggtatttg gttgaaaaac aaaatagtgt tattattgac
    23101 cacagggata tagataagga taataggtag tgtataatag atagttggta gtgggtagtg
    23161 aatagtggat agagagcagg taataggtga aatttatgat aggcgaaata ttcaacaagt
    23221 tattttacgg tatacttggc atgaaatccg ggactttatg taaacaaaaa aattgcagag
    23281 gactgacaaa tggcaggaag tctttgtcaa aaggtcctaa aatagttact ataggtggtg
    23341 gtaccggact atcaacaatg ttgagaggat tgaaattata tacctcaaac ttaactgctg
    23401 ttgtaacggt agcagatgat ggcgggggat ccggaatact tcgaaaagat ctgggaatta
    23461 ttccaccggg tgatatcaga aactgcattc ttgctcttgc tgatactgaa ccgctaatgg
    23521 aacaactact tcaatacaga tttgaggatg gaatgctaaa aggtcaaagt tttggcaacc
    23581 tttttctggc tgcaatgaac ggcatatcaa atggatttga agaagctgtg aagcgtatga
    23641 gtgatgtcct tgcagttacc ggtaaagttt tgcctgttac ccttggcaat gtacatcttt
    23701 gtgcacaact ggaaaatggt catataatta ccggtgaatc caatatcgga aagcataatt
    23761 attttgaaaa ggggacaata gaaaaagtat atcttgaacc ccggaatgca gcacctttaa
    23821 atgatgtttt agaagctatt tatgatgcag atgcaataat attaggaccg ggaagtctgt
    23881 acaccagtgt acttccaaac cttgtagtaa atggaataag cgaagcgctg aaaaaatcca
    23941 ttgcaaccaa aatatatgtt tgcaatataa tgacccagcc gggtgaaact gatgggtata
    24001 cagtatttga tcatataaga gcaatagaaa aacatactta taatggaatt attgattgtt
    24061 gtattgtaaa tacagcacaa attcctgcca aagtaagaac aaagtatctg gaggaaggag
    24121 ccgggatggt gaaaattgac tatgaccggc ttaaagaaac cagtacaagg ataatctatg
    24181 aggaactggt aagcatcagc aataattttt taagacatga tcccgccaaa ctggcagaaa
    24241 ccgctattga ccttattaac caaaccgcag ctccaaacaa aaaaagtata attgattact
    24301 tttttgcaaa ggatagtttg agaaaaacag caaaattatg agaaaagttg aggtgtaata
    24361 aggtatggaa ttcggtaagg gagactggag aagcttcgaa agaggcattg agaaggaatg
    24421 gctcttaact aatggaatag gagggtatgc ttcttctaca ataatcggtg ccaataccag
    24481 aagataccat gggcttttgg tagcagcttt aaatcctcct gtggagaggc atcttgctct
    24541 ggcaaagata gatgaaagca ttacaatagg agaaaataga gattataact tatattcttt
    24601 taagacatca aacggttata taatgaaagg ctataagcac cagcaaagat ttatatcaag
    24661 tcctcttcca gagtttatct acagtgttga agatgtaata attgaaaaga aagtaacaat
    24721 ggtgtatgga gaaaatacta ccgctattgt gtataggatt atcaatggct gccggcccct
    24781 taagcttaga tttgctccgc tggtaaattt cagagactat catcataatt cttccagatg
    24841 gcacatgaat tttaaaacaa atgtcattga aaatggagct ataattacac cttataattt
    24901 agaaacaaag ttatcactgg tatcagataa aggaatattt aaaaccatgg atgactgctg
    24961 gtttttaaat atgtattacc ctgttgagga ggagagggga ctttctgctg gagaagatca
    25021 ttttataccg ggatattttt atgttgaagc ccagccggga gaggaaaaat atgtaaccat
    25081 aatagctaca gtagaaaaag aagcaaaata tataaaagat ataaaagaaa gaaatgcgtt
    25141 aactcttata gagagggaaa aaggccgtat tgacagattg gtagccaggg cgggatataa
    25201 ggatgaattc agaaaaagcc tggttattgc tgccgaccag tttattgtca gcaggaaatc
    25261 cacaaattcc aaaacaataa ttgccggata cccctggttt actgactggg gaagggatac
    25321 aatgatagca cttcccggga ttacactggc aacaggacgt tttgatgatg caagggaaat
    25381 cctgggtact tttgctgaat acgcgaaaga cggaattata ccgaatatgt tccccgacgg
    25441 aggacagcct ccggcatata atacagtaga tgcttctctt tggtattttg aagcagtaaa
    25501 taaatttatt gaatacacag aggactatgc ttttgttaaa gacaagcttt acaaaacatt
    25561 gaaagatatt attaattcat atattaatgg cacacacctt ataaaaatgg atgctgactg
    25621 ccttttgaat gcaggaaatc catacacgca gcttacctgg atggatgcaa tggcaggcgg
    25681 gcgggcagta actccaaggt acggaaaacc tgttgagata aacgcactgt ggtataatgc
    25741 atttaagatc atgtcaaacc tggcagcgga atttggaaat gaaaaagaaa aagaatatta
    25801 tgaaaatcaa gctgatagaa ttcgagccag ttttgtggca aaattctgga atgaagcggg
    25861 acaatgtctt tatgatgtgg tcaatgaaga ttttatggat gataagatac gtcccaatca
    25921 gataatggcg gtaagtcttt catatccggt tgttgaaagg gatatggctg taaaaattgt
    25981 caaaaaggtg ttggaagagt tgtatactgc atatggcatt aggagtctgt ctccaaaatc
    26041 aaaagagtat aaaggtatat acattggaaa tccgtatgaa agggatagtg cttatcatca
    26101 gggtaccgcg tggtcatggc tgtcaggaca gtttataaca gcaatgatta atgcttacga
    26161 ggggagcagt gaaatccttg aaaaagccaa gctgttaata tggcctttca aagatcacat
    26221 aagggatggt tgtataggtt ctatttcaga aatatttgac ggagatgata cggggatacc
    26281 ccgtggatgt tttgctcagg cctggagtgt aggtgaagtt ttaagagctt acagcgaact
    26341 tgtgaggtga taaaattgtc attttcttct gttgtaaaaa atgagttaag caggattcat
    26401 gtggatgata aatgctgctt gaaagctgaa ttggcttcaa ttatagccgt aggtggtcaa
    26461 atggagatta gagagcctga aggccttaaa atcggaataa ttactgaaaa tgctgcattt
    26521 gcaagaagga gctactcatt gataaaggaa ttgtatgagg tttgcccgga ggttatatgc
    26581 agaaaaagca gaaaacttaa aaagcatact gtttttatac ttgcaattcc tgacacttca
    26641 tctataaaga aggaactgga tatacctgta aaagatgcat gttgtaaacg tgcttactta
    26701 agaggggctt ttttagcagg aggttcaatc agcgaccctg aaaagacata tcatcttgag
    26761 attgccaccc gttattccac tcttgcacgc cagttaagag acactattaa cagctataac
    26821 cttaatgcaa aaatcataaa aaggaagggt tattatgttg tttaccttaa agaaggtgac
    26881 catatagttg actttctaaa tattattggt gctcatgctg cattgcttga attggaaaat
    26941 ataagaatac taaaagatat gagaaataat gtaaacagaa ttgttaattg tgaaaccgct
    27001 aatcttgaaa agactgtaaa cgcttcggta agacaggtta ggaatattga atatataaga
    27061 gataatatgg gcttcggaaa tttgcctgag aatctcagag aaattgccga gttaaggctt
    27121 aaatacagcg atgcaagcct taaggaactt ggagaaatgc ttaatcctcc tttaggtaaa
    27181 tcaggtgtaa atcacaggtt gcgtaaactg gaggaaatag ctgagtccat ggcaagtcag
    27241 tcaggcagtc agaagtgcat ttaatttatt tgaaaaatta ttggcaaggg gcatacgtga
    27301 aggctctaag cgctgtaacg gcatccatgg taaatgcacc cggcagtatt gtcatataat
    27361 gacgaaagaa acttttatta ttacaaaatg taacattttt ctttgaagga atttctcata
    27421 tggatgaaga aatatatata aatagcgaaa atgattcaat gttaaatcat ttttgcatta
    27481 tctataaata aacaaccgga ggggggagaa tgttgcagat aaagttttct aacagaggta
    27541 atactttaat tgtacgactt aacggagaat tggaccacca tgcggcagat gatgtgaaga
    27601 gaaagattga taatgaaatg ataaaaccgg cttgcaaaaa tatagttttt gatttatcaa
    27661 agctaatttt catggatagt tcaggtattg gagttattat gggaagatat aaaaatatat
    27721 ataaaacaaa tggtaagatt atgatcgtat gttcaaatcc acatattgtt aaattactgg
    27781 aaatgtcggg tataccaaag atcataccaa tattcgacaa cgtagacaag gctgtaaaaa
    27841 aagctctgga ggtggcagaa tatgatgcaa aatgatgaaa caatggcttc atataaagga
    27901 aagccaataa atgaaatgac attaatattc ccggcaaagt catgtaatga gtcttttgca
    27961 agagttgtgg cagcttcttt tgtttcacag ctggacccga ctgttgaaga gctggctgat
    28021 gtgaaaacag cggtttctga ggcggtcacc aactcaataa tacatggata tgaaaaccag
    28081 acaggaaata taaaaatggt atgcagcctt taccaggatt ccattgaaat agaaataatt
    28141 gatgagggta atgggataga gaatatagaa cttgccatgc aacccttata tacctccaaa
    28201 cctgaaatgg agcgctcagg aatggggttt accgttatgg aaacctttat ggataaagtt
    28261 aacgttgaat cggaagtggg aaaaggaaca aaagtagcaa tgtgcaaacg cttcaaatca
    28321 attgggaaat agtaattaaa tagttgtata tgtataaaat aacacttata tggttataat
    28381 ttattattga ttaggtaaat tttggttaaa ataattacta tatataatgc aaaagtaatt
    28441 ttacaaggaa caaactggat ttttgcgcat taattaaggt aacataagcg cacaaaactt
    28501 ttataataca gtttcttttg tgcgttatct attaccttaa attaataccg actgctgaaa
    28561 atgtagtgtt tatgaactgt aaaatgtctg gaatcaagga gaatacaatg agtaattttt
    28621 ctgatgttaa tacttttgag ttcatcgaaa aagctaaaga aggggacaaa tatgctcaat
    28681 ctttgttgat tgagaaaaat atgggattgg tgtggaacat agtaaaaaga tttcagaaca
    28741 ggggttacga agctgaagac ctttttcaga taggctgtat agggcttatg aaggctataa
    28801 aaaagtttga cagctcttat ggtgttaagt tttcaactta tgccgtacct atgataatag
    28861 gcgaaataaa aaggttttta cgcgatgatg gacttattaa agtcagccgc tcattaaaag
    28921 aaacatcaag caaggtaaga ataataaagg aaacaatgat aaaggaactg ggtagagaac
    28981 ctactgtcaa tgaaattgca gaatcactgg acatttcacc tgaagaaatt gttatggcgc
    29041 ttgaagcaac ttatactcct gaatatttat atagtaatgt agctgaaggg gacagttctc
    29101 caattttatt aattgataga ctggatgctg agtcaagcct ccctgaaact gaccttatag
    29161 atagaattac attgagacag gttcttaata ccttaaatcc aagagaaaaa caaataattg
    29221 ttctcagata ttttaaagag aaaacgcaaa ctcaaatagc taaaatgctg ggtatttccc
    29281 aggttcaggt atcaaggctg gaaaagaaaa ttctaaagga attaaagaat aaaattaact
    29341 tcaaatcata attaaatgct tattaacttt tagtaaatgc atctatatat taccgcaata
    29401 aattcttgaa tattgagctt atcttttaat catactatag ataacgcaca aaaaataata
    29461 cattataaat tttttgcgct tatgttacct ttaataatag tattccgttt catgttttat
    29521 atttgggtca gattgtattt aaaagaccga agtttatgga acttatatcg gtactcatat
    29581 tatttttgat aggagtgaga taagcatgac tgaagtttta agaaaatata agtcagtgtt
    29641 tataatattg ggttttacat taatagcggc tgttattgcc tttattattt ttttatcagg
    29701 aagaaataca accaaaatcc ccttgagagg agtattcgta gatgaaacta atttccagaa
    29761 aaaatatggg gagaaggtgg cggaaggagt tggaacaggg ttatggctaa aaaaatgcta
    29821 aaagacttga aacctgcgga ataccgtaat tatgttgaaa gcaaatcacc aaaatccaaa
    29881 ctattaacta atgtattcag ggcttttttg attggcggac taatttgtgt agtgggacag
    29941 tttataacta atgcacttaa gtcaagaggc tatgatagtg atactgtagg tgccctcaca
    30001 tcagtaataa tgattttcat tggagctttg cttacaggtc tgaacattta tgatgaaatt
    30061 ggaaaatatg ggggagcagg ttctgctgtt cctataacag gatttgccaa ctccatagta
    30121 tcaccggcgt tggaatttaa aagtgaggga tatattatgg gagttggagc aaaaatgttt
    30181 gttgtagcag ggccggttct tgtatatggt atttcagcat ctattgtagc cgggatactg
    30241 tactatcttc taaagtgaga ggtgatttta tggcagataa aaggttggga aaacaaacag
    30301 taaaactcca aaatcctcct tcaattgtat ctacagcatg cattgttggt cctaaagaag
    30361 gcgcagggcc tcttggagaa tactttgaca tggtaattga tgatgaactg tggggtgaaa
    30421 agacctggga gaaagctgaa agcaaaattg tgaaagagac ttatttgaga atattgaata
    30481 aagcttctgc tacacctttt gatgtaaatt atatagtggc tggagacctt ttaaaccaat
    30541 gcatagcttc aactttcgga ttaaggggat ccagcgtatc acattttgga atctatggag
    30601 catgctcaac aatgtcagag tcaatgaata taggagcaat gctcattgat ggaggttttg
    30661 cagataatgt attatgtata acatccagcc atttttgttc agcagaaaaa caatttagat
    30721 ttccacttga actgggaaac cagaggccgc ctacttccca gtggacagtt ataggaagtg
    30781 gaggagcact attgtcaaat aaaggagcag gaccgtatat tacccatgta acaacaggaa
    30841 aaatagtaga tatgggaata actgatgcta ataatatggg aggagcaatg gcgcctgcag
    30901 cagcagatac aatattgtgt cattttaagg atacaggatt tgcacctgaa tattatgacc
    30961 ttattgtaac tggtgatctg ggaataatag gcaaaaccat ctgcgaagaa atggttaacg
    31021 aacagggttt ttctattagc gacaggttta atgattgtgg tttaatgata ttcgataaga
    31081 aaagacagga tgtacatgca ggcgcaagtg ggtgtggatg ctctgcagta gtgtttgctg
    31141 gttatattta taaagaaata atgaaaggca cctttagaaa agtgctgctg gtatctaccg
    31201 gtgcactctt aagccctaca agttcacagc aaaatgaatc cataccttca atagcccatg
    31261 cagtaaccat aagtaatact cttgaataga gggtgggagg agacaaataa tgttttggaa
    31321 ttatttatat gcttttatta caggaggaat aatttgtgtt gttggtcaat tacttattga
    31381 taagacaaaa atgacatctg caagaatatt agtacttttt gttactaccg gag
//
LOCUS       DAOSDD010000022        30551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_719_length_30551_cov_5.147101, whole genome shotgun sequence.
ACCESSION   DAOSDD010000022 DAOSDD010000000
VERSION     DAOSDD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 30551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30551
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_719_length_30551_cov_5.147101"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK733_03595"
     CDS             complement(<1..340)
                     /locus_tag="PK733_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09659.1"
                     /translation="MKYLQYYVIKGLYFRYFEKKVREAINYEIDTVEISDAVSQIALE
                     GKNDKFISVIEKTLNKLSNRGWSQAELAARLCILDYISFDEKYIKLIMLSYLMLSNVY
                     MVKSEYEVENG"
     gene            467..1645
                     /locus_tag="PK733_03600"
     CDS             467..1645
                     /locus_tag="PK733_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOJ09660.1"
                     /translation="MNYNFDKIINRKNTNCEKWDMIEKSFGSSDVLPLWVADMDFEAP
                     LEVAESIKKRTEHGVFGYTAVPYSYYKAVIDWLKVRHGWEVDRDWISLSHGVIPSLCA
                     AIITFTNPGDKILIQSPVYPPFFSIVKNNGRCLVNNQLKISQGKYIIDFDNLQKQLSE
                     GVKMMILCNPHNPVGRVWSRDELNQIGELCLKYNVMLVSDEIHSDLVYPYVKHIPAGS
                     ISEEIAQNSITCVAPSKTFNIPGLGISAVIIPNSRLSKMFNKTVSNMAMNMSSIFGPI
                     AAEAAYKYGADWLDQLLVYLQGNIDFLTQYLKDNIPQIKFFKPEGTYLAWLDCRDLGL
                     DQKELKSFMTNKAKVGLIEGTAFGPGGEGYQRLNFACPRSVLVEALQRIKAAVNDRTT
                     "
     gene            complement(1854..3113)
                     /gene="ahcY"
                     /locus_tag="PK733_03605"
     CDS             complement(1854..3113)
                     /gene="ahcY"
                     /locus_tag="PK733_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOJ09661.1"
                     /translation="MQYDIKDISLAPKGKLRIEWADQQMPVLRLIRERFAREKPLLGR
                     KMSCCLHVTTETANLMRTLKEGGADIVLCASNPLSTQDDVAASLVEHYDIPVFAIKGE
                     DRDTYYKHLVAAIEHGPEITQDDGADLVGLLHQLYKKSYLKKIIGGTEETTTGVIRLK
                     SMERDGALEFPVIAVNESDTKHLFDNRYGTGQSTIDGLLRATNILICGKNFVVCGYGW
                     CGRGLAMRAKGMGANVIVTEVDPVKAIEAVMDGFRVMKLEQAMPIADIVITVTGNLNV
                     VDSHHIEAAKDGVIIANSGHFNDEINIDALEKLASSKRQVRDYVVEYILKNGRKVFLL
                     AEGRLLNLSAAEGHPAVVMDMSFANQALGAEYIVKNEDRLEKKVYVLPENIDKEIARI
                     KLLSMGMEIDKLTLDQEKYLSSWKSGT"
     gene            complement(3344..4333)
                     /locus_tag="PK733_03610"
     CDS             complement(3344..4333)
                     /locus_tag="PK733_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOJ09662.1"
                     /translation="MKNHKPLLAFEGKTKEDWEEWRAKAYPKFMELLGEFPEKVDLEP
                     EVEYQVEECDLMRERVVFNTEEFMSVPCQILYPKNMKKDKSNAAIICSHGHGRFGKDP
                     VAGIRSSQGHIQDIKDMNYNYGEQMAKAGFLTISPDLRVFGERRDGENPFPGRDPCNV
                     NFIKGAIMGLYTLALNIWDIKCCVDYLETREEVNPERIGMMGLSQGGTMTTFATAVED
                     RIKAADIIGYVNPWAGFGIDKANFCGSQIVPGIYKYFDTDEIAGLIAPRPLLLEMGVY
                     DNCFYIQDLLKGYEGVKEIYRAAGIEEKLWTDIHPGPHAFGGNKAVEFFRKYL"
     gene            4813..6510
                     /locus_tag="PK733_03615"
     CDS             4813..6510
                     /locus_tag="PK733_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09663.1"
                     /translation="MRLLFTIKKPVKTQLKNLLLYFAQFLFILTSLLAGLCLFLLAAI
                     FFINFTFMNPIFQRKILEESGTYIQLDYIVQDNINKLYESGASESKVSETDISGSNFS
                     ESNLPGANLPGSVPGENQFKDILDNSYICINNETVKKGVNSFIEGLIKYIRSESRLLP
                     DIYIGRQVLHTLPANTPPADSISSNALPAISIPAEIDKINLGTILLFLNKSYLVDILI
                     VMQQVFQLMEPVPLTLSFLILLFAFIGIIISEKAADILKWWKLHFVSSGIIGLTSILI
                     VFSYSNKYLSSYNYLHILPYSEINVSGVITSYMQIFRMYLVYSMLIYSTICVLISISL
                     PGITGCIKSLYNAIRNYFFVGSEKTPGINLIHIRHISMIIVTLVAVFLIQNIYYLKEN
                     YKLNNPAESMARIVNSGAIADVISAKDDMIYSLELKISDEVTGKPISDIKVRVTEIVS
                     TENISSQVISSKVLPSEEAHSGVLPSDDLHSEIAPPEILSSKIMHYDIILSETDYKAE
                     TITDNTGTANMILDKGTYRLELSSEEKEYALPEPFLFDIELAGKNSIALRVSHLDTQ"
     gene            6615..>6955
                     /locus_tag="PK733_03620"
     CDS             6615..>6955
                     /locus_tag="PK733_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09664.1"
                     /translation="MPENWCPFFRKGVGTNIYQQPGPMSPPALPGMEQAYPLNMFPEQ
                     AMPEQVMPVQAMPGQAMQGNIWPGQMFPGQISPYMMQPCPNIYQSQSIHMHPYMGMAE
                     TYGMGNMGYLD"
     assembly_gap    6956..7055
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7084..7608
                     /locus_tag="PK733_03625"
     CDS             7084..7608
                     /locus_tag="PK733_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09665.1"
                     /translation="MDDMDDMDDMDDAEMKAAVSDPPPVLSNNPAVATISLFKELTGY
                     PNYGNPSGNADILYTGNRGAWTFQVPTLISALGNVRAQIFIRAVLDDHSNVPVDRYSA
                     RITINGTVVHTGRVPLEHGRPEGKQFNNWRLLTFDVANLRRNNRIVIVNTSNTGENDW
                     IGLDWMEMRFTPPR"
     gene            complement(7840..9216)
                     /locus_tag="PK733_03630"
     CDS             complement(7840..9216)
                     /locus_tag="PK733_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOJ09666.1"
                     /translation="MRKSSKNDVTKVILDECHIPKQWYNIVADMPNKPAPYYSPVTGK
                     IATPDDMSAIFPKELIMQEAATERWIDIPQKVREMYLQWRSTPLYRAKGLEKLLDTPA
                     RIYYKYEGTNATGSHKLNTSIPQAYYNKAEGVKRLATETGAGQWGSALSLACSQFGMD
                     CTVYMVKVSYQQKPYRRSFMKTFGADVIASPSNTTNSGRGILAKDPDSSGSLGIAISE
                     AVEDAVTHNDTNYALGSVLNHVCLHQTIIGLEAKKQMEMIDEYPDVIYACVGGGSNFS
                     GIAFPFLMDKFNGKKVRAVAVEPSACPTLTKGVFAYDYGDTAKMAPIAKMYTLGHDFV
                     PAGIHAGGLRYHGASKIISQLYHDGLIDAVAYGQKVVFDAAIMFARTEGIIPAPESSH
                     AIRAAIDEALRAKEAGEEKVILFNLSGHGYFDMSSYDYYFSGEMKDVEYTEDKVKQSI
                     MNLPQIEE"
     gene            complement(9421..10620)
                     /locus_tag="PK733_03635"
     CDS             complement(9421..10620)
                     /locus_tag="PK733_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ09667.1"
                     /translation="MIYIVNIHVIREIISMIHKLVKNNVYKNKNSRFNIKDKGSVTLE
                     AAIAIPVFISVIMSISLFIKVIYTHEIIQNAISHTANELASLSYIYHASGIEGLHNEI
                     LEKFKDIPDIHIEVDSSTDIPAEFDIPPELREILYNVLKGIYEDSFNQLCIPIARLCS
                     KKYLTTYEVKDPDNRLRMLDIIDGFKGLDFSMSEFFEEDDNIDIVVRYKVKIPAPVRI
                     FPSICIIQKASVRAWLGGDEIIEKDEKGEDDIWSLDNFSRGRKIRELYGANLPFNLPV
                     IARFEKGTATMIKSMDLTADSYQEPELVSKKVELYINELLGYQGQEVPWGKDQIIIKP
                     GDIKVRQLILVIPGNPIEPKVINTLEECRLKAGTEGIILKIEKYGYKKNEKAEQKENA
                     EEKDETE"
     gene            complement(10624..12900)
                     /locus_tag="PK733_03640"
     CDS             complement(10624..12900)
                     /locus_tag="PK733_03640"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HOJ09668.1"
                     /translation="MNLNNNKGQIAIFLAIVFISVLFLCGILIDRARIIGGRANVNIA
                     VEDALRSTMALYNSRLKNDYGIFALSENDDKALEDTVKEYISKNLSIDVNRYYRKKSS
                     DLHKSEDYPIDLESFMDYPIDLYDFRIEELSVAPIYNLTGNDALKKQILEYMKYRAPK
                     ELGENILKKLDIIKDAGKMAELYEKKVDIERELEDIDSIQRKIKKNISGTIGDGIFEP
                     SYIEKFNKDGERDNLVNRYVGLIERYASLESAIESGDEEECNDYDMKIMERMKIKKEI
                     QSVFKELTGRYTIEFTDINEEAIANIAKLIKQGKKISTEIKELCKTAEFKLHNTEISD
                     DIEFREAFTEDINNLMKLVPDIQEGQKKIDILKNNISALYTSIDNFNDIQNKVEMEDI
                     TGVDVFEIEKNLKDVNSNYSTIEFNYKLHPKTGDQDDPRKGLGQRLKNVLTEVVRNGL
                     DIEEKDIDFSELPSRKKVKDKDFSEEDWKYESSLDRLDKEVDLVNTKTTFPQKALNFI
                     IGAGNTIGENLINLRDDIYINQYIIDCFNSRANNADSSRIDKAGNSGADKADDIKDKY
                     KSCFQAEIEYILHGSELEEINVLKTMGQVVLIRFGINTLQIYTDAIKREQAYSLAAVL
                     AGWWTGGAGIPIIANMIICSWGMGEAMNDLRVLMEGKSIPFFKKYNITFSYEDYLWLF
                     LLMKKTDEKIDRIQDLIELNIRKEKPDFKMGNSHTAIRVEVAVSMKYLFATRPFMPSR
                     VKTSDGRHKIYVLLYEGY"
     gene            complement(12936..13667)
                     /locus_tag="PK733_03645"
     CDS             complement(12936..13667)
                     /locus_tag="PK733_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOJ09669.1"
                     /translation="MIREISNIFNSKKGSFTIETAIIIPFVFFCIIGLLIIPVMLYRK
                     ASVQILTDLCARRGARVWDDMNTDINTGKQLDSQQGKSSSGAEHKDSDDYSGGSEYEN
                     IPEYEYKYSLYWNLYDKYKGKKEIKLKNWLLQQLKNNYILYDENANVDIKLKDYFIFK
                     KLNSQVTGYYNLSAGIFPESFLIVKSDSEVIINKPWEFIRNVDFIIDIERQFEESYPG
                     FKEFMEKIRGTMDDIKKKIGEMDLF"
     gene            complement(13982..15868)
                     /locus_tag="PK733_03650"
     CDS             complement(13982..15868)
                     /locus_tag="PK733_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOJ09670.1"
                     /translation="MKTVIVALNSKYIHSALAPWYLKYACSNCREEIKILEYSINDDI
                     EWILSKIFLEKPDVAAFSCYIWNITHILKIAGNLKKVLPNIIVILGGPEVSYEPQEIM
                     GKNDFIDYIICGEGEESFQKLLSCLYSATNSSTFPAAMAADNLNIGEVEGLCYRNNGE
                     NCNQSSEEDVEKYKKIIYDGKYCLIKDLDSIPSPYTDEMLGSLNNKIVYYEASRGCPF
                     SCSYCISSTFDGVRYLSMDRVKKDILRLIGKGVKQVKFVDRTFNCNKKRAKEIIRFVI
                     EQSKESNYETNFHFEAAGDLFDDEMIQLLSTASEGVIQLEIGVQTTNEETLDEINRRT
                     DTEKVFDNIRLLKSEDNIHIHLDLIAGLPFENYISFQESFNRVYALEPHQLQLGFLKM
                     LKGSHIRNYSEKHGYKFREYPPYEVLNNKYISFSEMSELKGIEEVFERYYNSGRFKHS
                     LKFVIGYFYDSPFDFYRDFYLYNFNEGHLDKPLSARDLYTVFAGFAGNLEKLMVNAGK
                     IADCNISIYSIMNQFLKLDFLSTNNTGNLPEGINRTIEPGFKEKCHDFLKDSENISRY
                     CPHYKNISAGQILKRVQFETFEYDILSGNKIENIEKKKVTVLFDYTKKHKVTGVYSYS
                     RVSL"
     gene            complement(15968..17242)
                     /locus_tag="PK733_03655"
     CDS             complement(15968..17242)
                     /locus_tag="PK733_03655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ09671.1"
                     /translation="MKNLKSTRKIFIVLTILLIFNFIGMFLPLPDRIHEGKTLLPVIL
                     APRVALADEYCQDTIQSNIFETEDCTTPAEQPPVINALSAVVMDLDTGRVLFEKNAYK
                     RMPMASTTKIMTAIVAIENGNLDDEVTVSKLASGIWGSVIDLYEGEKIKHQDLLYGLM
                     LSSGNDAAIAIAEHVGGSLENFLEMMNQKARLIGARNTSFKSPHGLDKEGHYSTAYDL
                     AIMTAYALNNPVFSKIVGTKSITIGGNRSFYNTNEMLDMYPGTNGVKTGYTGQAGRCL
                     VTSVNRDGRRLVSVVLNCPSRSVRAISSKKILDYAFNNYTNYTLQEAGEIIASLPVVK
                     GLEDSVKVGTAEKVVLPLRHDELERLEKKVLLHESLEAPVYAGMDVGQIKFMLDEKVL
                     AECVLKTWADVQRKDFRYYIELIIKQWLKLTD"
     gene            complement(17533..17694)
                     /locus_tag="PK733_03660"
     CDS             complement(17533..17694)
                     /locus_tag="PK733_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09672.1"
                     /translation="MRCKDCINFKTSSRMEASNELSPRMGSLKGQCSVNNSACRAQDE
                     CRCGGFSQK"
     gene            complement(17723..18001)
                     /locus_tag="PK733_03665"
     CDS             complement(17723..18001)
                     /locus_tag="PK733_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOJ09673.1"
                     /translation="MMMKNITDKEITNVILDQHKLSASSLTTLVLESANQNLRNDATN
                     ILNKTFQHQKQLFDIMSQKGWYKTQAASQQQISQAQQEMNQMQSNMMM"
     gene            complement(18008..18205)
                     /locus_tag="PK733_03670"
     CDS             complement(18008..18205)
                     /locus_tag="PK733_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09674.1"
                     /translation="MALTTKELLLIQDNIKMTQNTVKFMEGCLEMTADPQVKGICQQM
                     IKDHQNDIQVLMKHINTTTMQ"
     gene            18376..19056
                     /locus_tag="PK733_03675"
     CDS             18376..19056
                     /locus_tag="PK733_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOJ09675.1"
                     /translation="MLIVFVRTLILYFIVVVVMRIMGKRQIGQLQPFELVVAIMISEL
                     AAVPMQDTAIPLLNGIIPILTLLIAQMLLSFISMKSIKARGIICGTPSVLIENGKIRE
                     ENLRKEMYTLNDLLEQLRTVNIPNIADVEFAILETNGQLSVIPKSQKRPVTAEDLSIP
                     TKYEGLPLDLIIDGRVINKNLDKANLDEKWLGNQLHVHGVHNIDNVLFASLDTSGNLY
                     FQEKMPTN"
     gene            19135..19527
                     /locus_tag="PK733_03680"
     CDS             19135..19527
                     /locus_tag="PK733_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HOJ09676.1"
                     /translation="MNDFIKVVLIVSILLTSIIGSGIYTNSILARDSNLMEERITQME
                     NHLKGGDWQSAEKELVYVKEYWSKKQGNWAVLQSHFEIDNIDASFTKVTEFINSKELS
                     LALAESALLKQFIMHIPKNVSFSLENIL"
     gene            complement(19561..20376)
                     /gene="rlmB"
                     /locus_tag="PK733_03685"
     CDS             complement(19561..20376)
                     /gene="rlmB"
                     /locus_tag="PK733_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOJ09677.1"
                     /translation="MQLIKSENNPLIKEVKALKTKKYREQKKLYFIEGLKFVEEALLS
                     GADITKIFISETLEINGKISGIKLITSKEEPIEYDIYVVPDNLFKGMSDTGTPQGILA
                     VVKMRNYTMDDIIALKNNKDNLFVVLDSIQDPGNMGTIIRTADAADFTAIIVSKGCVD
                     FYNPKVLRSTMGSIFHIPVVFCDNLADVLKYFRSQGIKTYAAHLNGTVSYFNVNMSNN
                     AAIIIGNEANGISNETASLADIFIKIPMPGSAESLNASVAAGVLIYESVRQRS"
     gene            complement(20581..20937)
                     /gene="rplT"
                     /locus_tag="PK733_03690"
     CDS             complement(20581..20937)
                     /gene="rplT"
                     /locus_tag="PK733_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOJ09678.1"
                     /translation="MARVKGGVTTRARHKKVLKLAKGYFGEKGKNYRIANQAVMKSQV
                     YAYRDRRAKKRDFRKLWITRINAAARINGMNYSRFMNGLKKAGINLNRKMLAEMAVND
                     SQAFAKLVETVKTAQN"
     gene            complement(20952..21149)
                     /gene="rpmI"
                     /locus_tag="PK733_03695"
     CDS             complement(20952..21149)
                     /gene="rpmI"
                     /locus_tag="PK733_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOJ09679.1"
                     /translation="MPKIKTHSSSKKRFNFTGTGKVKRFKAYKSHILTKKTTKRKRNL
                     RKGTTVSSADYSKVKQMIPYK"
     gene            complement(21237..21728)
                     /gene="infC"
                     /locus_tag="PK733_03700"
     CDS             complement(21237..21728)
                     /gene="infC"
                     /locus_tag="PK733_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOJ09680.1"
                     /translation="MVNEEIRDKEIRLIDVDGNMLGVMSAKEAQKLANAKNLDLVKIA
                     PKAVPPVCRIMDYGKYMFELSKKEKEARKNQKVITVKEIRISPNIEDNDFNFKVKNTI
                     KFLKDGDKVKVMVRFRGREINYTSIGQEILDKFAEAVKEYGVTEKRPKLEGKSMFIIL
                     NPK"
     gene            complement(22231..24138)
                     /gene="thrS"
                     /locus_tag="PK733_03705"
     CDS             complement(22231..24138)
                     /gene="thrS"
                     /locus_tag="PK733_03705"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ09681.1"
                     /translation="MIQVTLKDGSVNEYNKGITIKEVAVSISAGLARVALAGEVDGEV
                     KDLSYKLEKDCRLSLLTFEDEGGKDAYRHTSSHIMAQAVRRLYPDVKLAIGPAIDTGF
                     YYDFDKDKPFSVEELERIEEEMKKIIKEDLPLERFTLPRDEAVKFMEEKGEPYKVELI
                     KDLPEGEEISFYKQGDFTDLCAGPHLPSTGKIKAFKLLSVAGAYWRGSEKNKMLQRIY
                     GTSFPKKSELDEYLFRLEEAKKRDHRKLGRELDLFDIMDEGPGFPFFMPKGMVLRNLL
                     EGYWREEHKKRGYQEIRTPIILNEDLWHRSGHWDHYKENMYFTKIDDGNYAIKPMNCP
                     GGMLVYKRKLHSYRDLPQRMAEMGLVHRHELSGALHGLMRVRCFTQDDAHIFMTPEQI
                     KDEVQGVIELIDDFYKVFGFKYHVELSTRPENSMGSDEDWEMATNALRDAMESIGMKF
                     KINEGDGAFYGPKIDFHLEDSIGRTWQCGTIQLDFQMPERFDLTYIGADGEKHRPVMI
                     HRVVFGSIERFIAILTEHFAGAFPVWLSPVQVKVLPIVDKHYDYARSVQQKLEEIGIR
                     VEIDLRNEKIGYKIREAQLEKVPFMLVIGDKEMENGAVAVRSRKDGDLGAMLLDNFIS
                     KITQEVNGKAL"
     gene            complement(24724..24960)
                     /locus_tag="PK733_03710"
     CDS             complement(24724..24960)
                     /locus_tag="PK733_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09682.1"
                     /translation="MENIARRPDSVIMQEGMNVLLKKLGKVDAERFISLIIREPFDYT
                     KWQANLFEGMSLEELSMKAMDYYNKKIKDKDTGR"
     gene            complement(24964..25392)
                     /locus_tag="PK733_03715"
     CDS             complement(24964..25392)
                     /locus_tag="PK733_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOJ09683.1"
                     /translation="MKIYLDNCCFNRPYDDQTQLKIYLETQAKLDIQKDIISGEYELV
                     WSFILDFENEQNPFELRKNSIAGWRSVAKSYIILNDNISNFAKLLLEKGLKKKDALHI
                     ACAVEAKCNVFLTTDKKLLNTLIPEIQVLSPLEFISKMEG"
     gene            complement(25690..27264)
                     /locus_tag="PK733_03720"
     CDS             complement(25690..27264)
                     /locus_tag="PK733_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOJ09684.1"
                     /translation="MITKNIADKHKRVVPLPKKAVFNGDFIKLNDNWYIELDKESGKV
                     CKTDGIQQRCSSITNIAEKWSRKFGMAGVSHGGETGLNGSCIGKISLHTDNNMNSEGY
                     KINIGPEQVNISAGSEKGIDYAMQTLWQLSPDGNIPAGNIEDKPSIKIRGFHINFDSF
                     RQMDIEEALRSIETAAELKLNTILLEYADRFPYEKHSRIAVNTTLKKEDIKRINDKAK
                     ELGIELIPLQQTIGHLEYILEHDYYKSIRENRETISQICPLNPDSFELIKELLEEMIE
                     AHPGIRYIHIGGDEARSLGKCPSCKEKVEKYGISRLYIDHINSICDFITSKGITPIIW
                     DDVLCAHPEALDLLDKRIVIMYWDYWTVSKSSPYFIARYDRKGRPVTVCDAGWDNEWK
                     HELTDLERSIMSTFASKVPLKESLSKKFIELYGPYLGNEFPKRIKGYPYLEFFQDKGF
                     KVIGAPTSLGNGDDYNTLPNYWRFIPNIRTAAERCIEANAEGLVTTAWYNYHPVMFHM
                     GLGATAQFSWGVEKEL"
     gene            complement(27324..28541)
                     /locus_tag="PK733_03725"
     CDS             complement(27324..28541)
                     /locus_tag="PK733_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09685.1"
                     /translation="MKGGITIEEKGVKLGLIGLGPRGRTLMGILVHIEEIQITAICDA
                     DFAHFETIFKYFDDADLQRPDTYTDYNELLARKDIEGVVIATNWITHIPLSIAAMKAG
                     KYVGLEVGGAASIEECWQLVRVSEETGIPCMMLENCCYGKYELMTLNMVKKGLFGEIV
                     HCECGYGHDIRTMAGRPNNRLYNNMHRNGDLYPTHGVEPIAKMLNINRGNRFISLTSM
                     ASKAVGLRDWIKKHKEPDYELANFDFVKGDVVQTMMKCAHGETVLVTHNVTLPRPYSR
                     FNVVHGTNGIYMEDNKGIFIDGKTEGEKWESIDDYYEEYGHPLWKEFEESGVKGGHGG
                     MDYLLLKAFAESIRNKTQTPIDVYDTATWMAITVLSEESINRGSSSVAFPDFTNGRWI
                     DRETAVKSKYSLC"
     gene            complement(28660..29883)
                     /locus_tag="PK733_03730"
     CDS             complement(28660..29883)
                     /locus_tag="PK733_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09686.1"
                     /translation="MKKYVIIGAGDRGLHMFGTRLSSDKFKGYASLVGVYDINSVRAN
                     EFSKDCGDILVFNDFDTMINETKPDVVIVTTIDSYHHEYIIKALEAGCDVISEKPMTI
                     DSEKCKAILEAEKRTGNKISVIFNMRFMPYASKVKELIKEGIVGEVLNVDLEWMVDTI
                     HGPQYFRRWHRYIEKSGGLLVHKSTHHFDLINWWIDQYPEEVFAFGTRRYYGPTRENR
                     GERCLACNHKKTCEHYWNINSSAHNSFYIRYFLNAEKEDGYIRDACVFAEDINIYDTM
                     SVNVKYSKGTLLSYSLIAHSPYEGYRAAINGTKGRLEIGEIFRGKGADQPYRQIKFFN
                     RNGDIIIYDIPKAVGDHGGGDDKLMRMLFIGDVDDPLGCQASSMDGAMSLLIGASANI
                     SIAEKRPIEINEQLK"
     gene            complement(29987..>30551)
                     /locus_tag="PK733_03735"
     CDS             complement(29987..>30551)
                     /locus_tag="PK733_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09687.1"
                     /translation="NWNANPSALTTGSFVITNENKYPIETVKWVDYFYTEEGSALYFL
                     GKEGVTHIKNPDGLDYGQATNKYTPGSVGDNPIVLLKNVFKGAEAQPIPLNAAEKMQK
                     YSLKEVWTQFDLTIEENERVRELSSDINSYVAKTTADFLTGRTPFTEWDNNIATLKKM
                     GLDEFLQIQQTAYERYVKNQNDHLALF"
BASE COUNT         8944 a         6174 c         4522 g        10811 t
ORIGIN      
        1 acccattttc tacctcatac tctgacttta ccatatacac attacttaac attaaatatg
       61 aaagcataat aagctttata tatttttcat caaaacttat ataatctagg atgcataggc
      121 gagcagcaag ctctgcttgc gaccagcccc ggtttgaaag cttatttaat gttttttcga
      181 ttactgatat aaatttatcg tttttacctt caagtgcaat ttgagataca gcatcactta
      241 tttctacagt atcaatttcg taatttatag cctctcttac tttcttttca aaatatctaa
      301 aatataaacc tttgataaca taatattgta aatatttcaa tataaattgc aatagtatgt
      361 tgctttttta gttgttaaat tggtaaaatt atattcagtg gtaattacag cagctttttt
      421 gctgctgatt tattacaacc atattgtacc agggggggac cgcaaattga actataattt
      481 tgataaaata ataaaccgca aaaataccaa ttgtgaaaaa tgggacatga ttgaaaaatc
      541 attcggcagt agtgatgtat tgccactgtg ggttgcagac atggactttg aagcgcctct
      601 tgaagttgcc gaaagcataa aaaaaagaac tgagcacggc gtttttggat atactgcagt
      661 accgtactcg tattacaaag cagtgataga ctggctcaag gtccgccacg gatgggaagt
      721 agacagagat tggatttcat taagccatgg tgttattcca tcattgtgtg ctgctataat
      781 aacttttaca aaccctggtg ataagatcct aattcaatca cctgtttacc cgccattttt
      841 ttctattgta aaaaacaatg gacggtgcct tgttaataat cagcttaaaa tttcccaagg
      901 gaaatatatc attgattttg ataatctcca aaagcagctt tcagaagggg ttaagatgat
      961 gatcctttgc aatcctcata atcctgtggg acgtgtctgg agcagggatg agctaaacca
     1021 aataggagaa ttatgcctta aatataatgt aatgctggtt tctgatgaaa ttcactcgga
     1081 tctggtatac ccttatgtaa agcatatacc tgccggctca atttcagagg agattgctca
     1141 gaattcgatt acctgtgtag caccttcgaa aacctttaac attcccggac tgggtatttc
     1201 agctgtcatt atcccaaaca gcaggctttc taaaatgttt aacaagaccg tctccaatat
     1261 ggcaatgaac atgagcagta tttttggtcc aatagcggca gaagcagcat acaagtatgg
     1321 tgccgactgg ctggaccagc ttcttgttta cctgcagggc aatattgatt ttttaactca
     1381 atacctgaaa gataatattc ctcaaattaa gtttttcaaa cctgaaggta catatctcgc
     1441 ctggctggac tgcagagacc ttggattgga tcagaaagaa ttaaagagct tcatgactaa
     1501 caaggcaaaa gtagggttga ttgagggcac tgcttttggt cccggaggtg aaggatatca
     1561 aagacttaac tttgcatgtc caagatcagt acttgtggag gctctgcaaa ggataaaagc
     1621 cgcagttaat gatcgaacta cttaagtaag gagggcaact tgcccccctt gttcttagct
     1681 tcattaatat gactacttcc gacactcagc ctcccacttt aaactataag ctcttaaccc
     1741 tgtagtttaa atttcgactt ccagcctctc agttcaaact tccggctcct cactcctcag
     1801 ttccaacttt ccactaccca ttacacacta cccaatacac actacctact accttatgtc
     1861 ccgcttttcc aggaggatag gtacttttcc tgatccagag tcagtttatc tatttccata
     1921 cccatggata agagtttaat tcttgctatt tccttatcaa tgttttcagg cagtacatat
     1981 actttttttt ccagcctgtc ttcatttttt acaatgtatt cagcgccaag agcctggttt
     2041 gcaaagctca tatccataac tacagccgga tgcccttcag ctgcagaaag gtttagaagt
     2101 cttccttcag caagtaaaaa tacttttctt ccgtttttta atatatattc cacaacatag
     2161 tctcttacct gcctttttga tgaagcaagt ttttccaatg catcaatgtt gatttcatca
     2221 ttaaaatgac cggagtttgc tataattaca ccatcttttg ctgcttcaat atggtgactg
     2281 tcaactacat taagattacc ggttacagta ataacaatat ctgcaattgg cattgcctgt
     2341 tcaagtttca ttaccctgaa gccatccata actgcttcta ttgcttttac aggatccact
     2401 tccgtaacaa taacattagc tcccattcct ttagctctca tagccaggcc tctgccgcac
     2461 cagccataac cgcagacaac gaaattcttg ccgcatatta atatgtttgt tgccctcagc
     2521 agcccgtcta tagtactctg acctgtccca tacctattat caaacagatg cttggtatca
     2581 gattcattta ccgctattac aggaaactca agagcaccat ctctctccat actttttagg
     2641 cgtataacac ccgttgttgt ttcttcagtg cctccgatta ttttcttcaa atagctcttt
     2701 ttataaagct gatgcagcaa acccaccaga tcggctccat cgtcctgggt gatttccggt
     2761 ccatgttcaa ttgccgccac caaatgctta taataggtat ccctgtcttc cccctttata
     2821 gcgaatacag gtatgtcata atgttcaaca agtgatgccg ctacatcatc ctgtgtggaa
     2881 aggggatttg aagcacatag tacaatatcg gctccccctt cttttaaagt cctcattaaa
     2941 tttgccgttt ctgttgtcac atgaaggcaa caggacattt tccttcctaa aaggggcttc
     3001 tccctagcaa acctttccct tatcaatctt aaaactggca tctgttgatc ggcccattct
     3061 atcctcagct ttcctttagg tgccagagat atgtctttta tatcatattg catcattttt
     3121 tggcattcct tcccttattg aatgtatttt tgctgtatct gtactttttg tacatgtata
     3181 tttttctata ttttttgtgt ctacatttat tgtatcagta ttttgttatt attatttata
     3241 ttcattcaga ttgttagagt aaatagattt taaaaataca gataagaata ttgctatatt
     3301 tacatggttt tacacaagac ccaacaatat gctcatccta cggctataag tattttctaa
     3361 aaaactccac agctttattc ccgccaaaag catgaggtcc gggatgaata tccgtccaaa
     3421 gtttctcttc aatacccgct gctctatata tttcttttac gccttcataa cctttaagca
     3481 aatcctgtat gtaaaagcag ttatcataaa ctcccatctc cagcagcaac ggtctcggtg
     3541 ctataagtcc ggcaatttca tcagtatcaa aatacttata aataccagga actatttggg
     3601 aaccacaaaa atttgcttta tctattccaa agcccgccca tgggttaaca taaccgatta
     3661 tatccgcagc tttaatcctg tcttccacag cagtggcaaa tgtagtcata gtaccaccct
     3721 gacttaaacc catcatacct attctttccg ggtttacttc ctctcgtgtt tccaggtaat
     3781 caacgcaaca ttttatatcc cagatattca aggcaagcgt atacaacccc atgatcgcgc
     3841 ccttaataaa gttgacatta caaggatctc ttcccggaaa agggttttct ccgtcacgtc
     3901 tttccccgaa aaccctgaga tcaggggata tagtcaaaaa cccggctttc gccatctgct
     3961 ccccataatt ataattcata tctttaatat cctgaatatg tccttgagat gatcttatcc
     4021 cggcaacagg atctttgcca aatcgtccat gcccgtgact gcatattatt gcagcatttg
     4081 acttgtcctt tttcatattt ttagggtata ggatttgaca tggcaccgac ataaattcct
     4141 ctgtattgaa aactactctt tcccgcataa gatcacattc ttcaacctga tattcaactt
     4201 caggttcaag gtcaaccttt tcagggaatt cacccagtag ttccataaat ttaggatatg
     4261 cttttgccct ccactcttcc caatcttctt tggtcttgcc ttcaaaagct aataaaggtt
     4321 tatgattttt cactttatgc atccagtacc tatgcatgga aaaatttctt tcagccattt
     4381 ttcaattctt cctttcattg cttttttcta taatataact tatggtattt atcatgtccg
     4441 cgcctgcaga cgatcaagtt ttctgcaaca gcgttataac atttaataat tattatatag
     4501 catatgattt tgcatggcaa ttactataat tttttatagg gtacattttc taaatatgaa
     4561 gaaatattta ccaggggctg tcgttacggt gcctagagcc tttaaattca gctactggca
     4621 aaatcttcta cataaaatgc ttcatccaat aatttcttca taaacataat atatgctcat
     4681 ctttatatct ttattggttc ttttattgct ttggtttata tttccaatga ttaaaacttt
     4741 aataactgat ataataattt atgtaaagag acttttttgt tggcatttta tggtgaccca
     4801 ggaggaaaca aattgcgctt actgtttact attaaaaaac ctgtaaaaac acaactaaaa
     4861 aatttattat tatattttgc acaatttctc tttatattga catcgctcct tgcaggatta
     4921 tgcctttttc tgcttgcagc tatcttcttc attaatttta cttttatgaa tcctatattt
     4981 caaagaaaaa ttttggagga aagtggtact tatattcagt tggactatat agtacaggac
     5041 aatataaaca agctttatga atcgggagct tcagaatcta aggtttcgga aactgatatt
     5101 tcgggatcca atttttcgga atcaaatctt ccgggggcca atcttccggg gtctgtcccg
     5161 ggtgagaacc agttcaaaga tatactagat aattcatata tatgtataaa taacgaaact
     5221 gttaaaaaag gggtaaattc atttatcgaa ggtttaatta aatatattag aagcgaaagc
     5281 aggctcttgc ctgatatcta tattgggaga caggttttac ataccttgcc ggctaatact
     5341 ccgcctgctg attctatttc ttccaatgct ctacctgcga tttctattcc cgctgaaata
     5401 gataaaatta accttggtac tatattgctt tttttaaata aaagttattt agtagatatt
     5461 ctaatagtta tgcagcaagt atttcagctt atggaacctg ttcctctcac cttatctttc
     5521 ttaatattgc tatttgcttt tattggaata ataatatctg aaaaagctgc agacatatta
     5581 aaatggtgga aattacactt tgtttcttca ggaataatag gtttaacatc aattttaatt
     5641 gtattttcat actcaaataa atatttaagt tcatataatt atttacatat actaccatac
     5701 tcagaaatta acgtttcagg agttattact tcctatatgc aaatatttcg aatgtatctg
     5761 gtatattcaa tgcttatcta cagcacaatt tgcgtattaa tatctataag cttgcctggc
     5821 attaccggtt gcattaaatc attatataat gctataagaa attatttttt tgttggtagt
     5881 gaaaaaaccc cgggaattaa tttaattcac ataagacata tttcaatgat tattgtaact
     5941 ctagttgcag tatttctaat tcaaaatata tactatttaa aagaaaatta taagctgaac
     6001 aatcctgctg aatcaatggc cagaattgtg aattcaggtg caatagctga tgtaatatca
     6061 gctaaagatg atatgatata ttcccttgaa ctaaaaatta gtgatgaagt aacaggaaaa
     6121 cctatctccg atattaaagt tcgtgtaaca gaaatcgtat ctactgaaaa tatatcttct
     6181 caagttatat cttccaaagt tttgccttct gaggaagcac attccggagt tttgccatct
     6241 gacgatttgc attctgaaat tgcacctccc gaaattttat catccaaaat tatgcattat
     6301 gatatcatcc tttctgaaac cgattataaa gcagaaacaa taacagataa tacaggtact
     6361 gcaaatatga tacttgataa aggtacatat aggttagaac tgtcatctga agaaaaagaa
     6421 tatgccctac ccgaaccttt tctatttgat attgaactgg caggtaaaaa cagtatagca
     6481 ttaagggtct cacatcttga tacccaatag tgaaaaatta atgagtaaca taacaacgca
     6541 acaaggaata ttatgtataa gaaatagtgt tttacgtaaa ctctgtttga atttaaaagg
     6601 aggattgaat tataatgcct gaaaattggt gtccgttttt tcgcaaaggt gtaggtacta
     6661 atatctatca gcaaccaggc cctatgtctc cacctgctct cccaggtatg gaacaggcgt
     6721 accctttaaa tatgttccca gaacaggcta tgcctgagca agttatgcct gtacaggcaa
     6781 tgccggggca ggcaatgcag gggaatattt ggcccggcca gatgtttcca ggacagatca
     6841 gcccttatat gatgcagcct tgtcctaata tttaccaatc ccaaagcatt cacatgcatc
     6901 cttatatggg tatggcagag acatatggta tgggtaatat gggttattta gatgannnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatgg atgatatgga tgatatggat
     7081 gatatggatg atatggatga tatggatgat atggatgatg cagaaatgaa ggccgctgtc
     7141 agcgacccac ctccagtact aagcaataac ccggctgttg ccacaataag tttattcaag
     7201 gaattaacag gttatccaaa ctacggtaat cccagtggaa atgcagatat tctatatacc
     7261 ggcaacagag gagcctggac ttttcaagtt ccaacattaa tttctgcact tggtaatgta
     7321 agagcacaaa tttttatccg tgctgttttg gatgaccact caaatgttcc tgtagacagg
     7381 tattctgcaa gaattactat aaatggtact gtagttcaca ctggtcgggt acctcttgag
     7441 catggtagac ctgaaggaaa acaatttaac aactggaggc ttctcacatt tgatgtagct
     7501 aacttgagaa gaaataaccg tattgtaatt gtaaacacat ctaatacagg tgaaaatgac
     7561 tggattggat tggattggat ggaaatgaga tttacaccac cgagataaac aaagctccat
     7621 aagtcctgca taagtaaata ataatctgct aaaatagccg atattgtatg aaaatattag
     7681 aatgcaagaa tagagaatac aagatacaaa aatccaggtt ttgactcctg gattttttta
     7741 tgagcatttt ttgttattat tgcttaacct gctaaaactt ataatgctat tctccgggtg
     7801 cattttcttc ctatgataca aaatatagaa aatatgcccc tattcttcta tctgaggcag
     7861 gttcattatg ctttgtttta ctttgtcctc agtatattca acatccttca tttcaccact
     7921 gaaataatag tcgtaggatg acatatcaaa atatccatgt ccgctcagat taaatagaat
     7981 aactttctcc tcacctgctt ctttagccct taaggcttca tctatggctg ccctgatggc
     8041 atgcgaagat tcgggagcag gtataatgcc ctcagttctt gcgaacataa tggctgcatc
     8101 aaataccacc ttttgcccgt atgcaactgc atctataaga ccatcatgat aaagctggct
     8161 gattatttta gatgctccat gatatctgag acctcctgca tgtataccgg caggtacaaa
     8221 atcatgcccc aaagtataca ttttcgcaat aggagccatt ttagccgtat caccataatc
     8281 atatgcgaaa acacctttgg taagggtagg acatgcggat ggttcaacag caacagctct
     8341 tacttttttc ccattaaatt tatccatcag gaatgggaag gcaatccctg agaaattact
     8401 tccgcctccg acacacgcat aaattacatc aggatattca tcaatcattt ccatctgctt
     8461 tttggcttca aggcctataa tagtctggtg aaggcataca tgatttaaca cacttccaag
     8521 agcataattt gtatcattat gggttacagc atcttccact gcctcactaa tagctattcc
     8581 caggctccct gacgaatcgg gatcctttgc aagtattcct ctgccgctgt ttgtagtatt
     8641 actcgggcta gcaataacat cagcaccaaa tgttttcata aatgatcttc tataaggttt
     8701 ttgctggtag cttactttaa ccatataaac agtacaatcc atgccgaact ggctgcaagc
     8761 aagactcaaa gcacttcccc attgccctgc tcctgtttct gtagcaagtc tctttacacc
     8821 ttcagcttta ttataatatg cctgtggtat ggacgtgttt aatttgtggc ttccggtagc
     8881 gttggtaccc tcatacttat aatatattct tgccggcgta tccaaaagtt tttcaagacc
     8941 ttttgctcta taaagaggtg tagatctcca ttgaaggtac atttccctca ccttctgtgg
     9001 aatatcaatc catctttcag tagcagcttc ctgcataatc aattctttag ggaatatagc
     9061 cgacatatcg tcaggtgtgg ctattttacc ggttacaggg ctatagtatg gggcaggttt
     9121 gtttggcatg tctgcaacga tattatacca ttgctttgga atgtggcatt catcaaggat
     9181 aacttttgta acatcatttt tactcgactt tctcatctta actcatctct cctattctaa
     9241 tctaaatatt tttttaacag gctattcttc tgaagagcta tttaagaagc tctcaaaaat
     9301 ttagctttct gttaaaccaa atataattac taataatatt atcagtaatt gattcttgtg
     9361 tcaataggtt tttcatattt ttcccatggt ttttttgcca tttctactcg tcttttttgt
     9421 ttattcagtt tcatctttct cttcggcgtt ttctttttgt tcggcttttt catttttttt
     9481 ataaccgtat ttttctatct tcaatattat accttctgtt ccagctttta atctgcattc
     9541 ttcaagtgta ttgatgactt taggctctat aggatttccc ggtattacta aaatgagctg
     9601 ccttaccttt atatcccctg gtttaattat aatttgatcc tttccccacg ggacttcctg
     9661 accctgataa cctaatagtt cgtttatata caattcaact tttttagata ctaattcagg
     9721 ttcctggtat gaatctgctg tcagatccat acttttaatc attgttgctg ttcctttttc
     9781 gaacctggca attacgggaa gattgaaagg caggtttgca ccatataatt ccctaatttt
     9841 tctaccccgg ctgaagttgt ctaaggacca tatatcatct tcaccttttt catctttttc
     9901 tataatttca tctcctccca gccatgctct tacagaagct ttttgaataa tacatatgga
     9961 gggaaatatc cttacaggtg caggtatttt gactttatat ctaactacta tatcaatatt
    10021 atcatcttct tcaaaaaatt cagacatgct gaaatcaagg cctttaaagc catcaataat
    10081 atccagcatt cttaatctat tatccgggtc ttttacttca taagttgtta aatatttctt
    10141 ggaacataat ctggctatgg gtatacataa ttgattaaat gaatcttcat agataccttt
    10201 taatacattg tataaaatct ctcttaattc agggggaatg tcaaattctg cagggatatc
    10261 tgttgaagaa tccacttcta tatgtatgtc cggtatatct ttaaattttt ccaatatttc
    10321 attatgtaaa ccttcaatac cacttgcatg ataaatatag cttaatgacg caagttcatt
    10381 tgcagtatga cttatagcat tttgtattat ttcatgagta taaataactt ttataaaaag
    10441 tgatatagac ataattacac ttataaatac aggtattgca atagctgcct caagtgttac
    10501 agatcctttg tctttaatat taaatctact gtttttattt ttgtaaacat tgttcttaac
    10561 taatttatgt atcatactga ttatttccct gataacatgt atattaacta tataaatcat
    10621 taattaatac ccctcatata aaagaacata tatcttatgc cttccatctg aagttttaac
    10681 ccttgaaggc ataaatggcc gggttgcaaa caagtatttc atcgatactg ccacttcaac
    10741 ccttatagct gtatgtgaat tgcccatttt aaaatccggc ttttcttttc tgatattcag
    10801 ctctataagg tcctgtatcc tgtcaatttt ttcatcggtt tttttcatca ataggaataa
    10861 ccagagataa tcttcatatg aaaaagtaat attgtatttt ttaaaaaagg gtattgattt
    10921 accttccata agtactctca aatcattcat tgcttcaccc ataccccagg agcatattat
    10981 catattggct ataatgggga tccctgctcc ccctgtccac caccctgcaa gaacggcagc
    11041 aagactataa gcctgctctc ttttaatcgc atctgtatat atctgtaagg tattaatgcc
    11101 aaacctaatt aatactacct gacccatagt cttcaaaacg ttaatttctt ctaattcact
    11161 gccatgaagt atatattcaa tctcagcttg aaaacaggat ttgtatttat cttttatatc
    11221 atctgcttta tctgcaccac tgttgcctgc cttatctatt ctactgctgt ctgcattatt
    11281 tgctctgctg ttgaagcaat ctataatata ctggtttata tatatatcat ctcttaaatt
    11341 aatgagattt tcgccaatag tattcccggc accaataata aagtttaatg ccttctgagg
    11401 gaaagttgtt tttgtattta ccagatcaac ttccttatcc aatctatcca aactgctctc
    11461 atatttccag tcttcttcgg aaaaatcctt atcctttact ttctttctgg atggcagctc
    11521 actaaaatct atatctttct cttctatgtc taatccattt ctgacaacct ctgtcagtac
    11581 atttttcaat ctctgtccaa gaccttttcg tggatcgtct tggtcaccag tcttgggatg
    11641 cagcttataa ttaaattcaa ttgtactata attggaatta acatccttta gatttttttc
    11701 aatttcaaac acatctactc ctgttatgtc ttccatttct accttgtttt gtatatcatt
    11761 aaaattgtca atggaagtgt atagagcact tatgttattc ttaaggatat caatcttttt
    11821 ttgtccttcc tgaatatcag gtacaagctt cataaggtta tttatatctt cagtaaatgc
    11881 ctctctgaat tcaatatcat cggaaatttc tgtgttatga agcttaaatt cggctgtttt
    11941 acagagttct ttgatttcag tactaatttt ctttccttgt tttatcagct ttgctatatt
    12001 tgctattgct tcttcattta tatctgtaaa ttctatggta taccttccgg taagttcttt
    12061 gaaaacactc tgaatttcct tttttatttt catccgttcc attattttca tgtcatagtc
    12121 attgcactct tcctcatctc ctgattcaat agcactttct aaagaagcat atctttctat
    12181 aagccccaca tacctgttta caaggttatc acgctcacca tccttgttaa atttttcaat
    12241 ataacttggt tcaaaaattc catctccaat agtgccactg atatttttct tgatttttct
    12301 ctgaatactg tctatatctt caagctctct ttctatatca accttttttt catacagttc
    12361 tgccattttt cctgcatctt ttataatatc cagtttcttc aatatatttt caccaagttc
    12421 ttttggggct ctatatttca tgtattcaag gatctgcttc tttaacgcat cattccctgt
    12481 aagattatat attggtgcaa cacttagttc ttctattctg aaatcataca gatctattgg
    12541 ataatccata aaactttcca agtctatagg gtaatcttcg gatttatgta agtctgaaga
    12601 tttttttcta taatatctat tcacatcaat acttaagttc ttactaatat attcttttac
    12661 tgtatcttcc aatgctttat catcattttc ggacaaggca aatattccat agtcattctt
    12721 aagcctgcta ttatataaag ccatggtaga ccttaaagca tcttcaactg ctatgtttac
    12781 attagccctt cccccaatta tccttgctct gtctatcagt attccacata gaaaaaggac
    12841 agaaataaaa acaattgcta aaaatatagc tatttggcct ttgttattgt tcaaattcat
    12901 ccggtaccaa ctccaaacct actataactt tttacttaaa ataaatccat ttcccctatt
    12961 ttctttttaa tatcatccat tgtacctctg attttttcca tgaattcttt aaaacctgga
    13021 tagctttcct caaattgtct ttcaatatct ataataaaat caacattcct tatgaactcc
    13081 cacggtttat taattataac ttcagaatcc gacttaacta ttaaaaagct ttccggaaat
    13141 attcctgcag aaagattata ataacctgtt acttgtgaat tcaacttttt gaaaataaaa
    13201 tagtccttca attttatatc aacattagca ttttcatcat ataaaatgta gttgtttttt
    13261 aattgctgaa gcagccagtt cttaagcttt atttcctttt tacccttata tttatcatac
    13321 aaattccagt ataaagagta tttgtactca tattcaggaa tattttcgta ttcggaccct
    13381 ccgctatagt catcactatc cttatgttca gccccgctgc tggatttacc ctgttgagaa
    13441 tccagttgct taccggtatt tatatctgta ttcatatcat cccataccct tgcacccctt
    13501 ctggcacata aatctgtcaa tatttgaaca gatgcttttc tatacagcat aacaggtata
    13561 attagaagtc caataatgca aaaaaataca aatggtatta ttattgctgt ctctattgtg
    13621 aaactacctt ttttgctatt aaagatatta gatatttctc tgatcatttt atacaacccc
    13681 ttatacaact ccacaattaa tgaatgcatt aacacttgtt tcttattttg ttaaaatgtg
    13741 tattgtaacc atatattaca tcaattgtat tatttttgca ataggttttt taaatatttt
    13801 gcgtttatta aattaatact attatgccaa tttggaaagc tatatcatcc gggataattt
    13861 ttgataacat cgggaagatg cattccttac gggtcagcaa aaattaccct ccagaaaggg
    13921 tgctttccat tagcaacata acaaagtagt attaagtaaa tataaactaa atttgctcca
    13981 tctaaaggct tactctggaa tagctgtaga cgcctgttac tttatgtttt ttggtataat
    14041 cgaacaggac agtgactttc ttcttttcaa tgttttctat tttattacca gacaatatat
    14101 catattcaaa agtttcaaat tgaacccttt taagtatttg accagcagat atgtttttat
    14161 aatgagggca ataccggctt atattttcac tatctttcag aaaatcatgg cacttctctt
    14221 taaatcctgg ttctatagtt ctattaatac cttcaggcaa atttcccgta ttgttggttg
    14281 acaggaaatc cagcttcagg aactggttca taatactata tattgatata ttgcaatctg
    14341 ctattttacc agcattcacc attaactttt caagattacc tgcaaatccg gcaaaaacag
    14401 tatataagtc tcttgcagaa agaggcttgt ccagatgccc ctcattaaaa ttgtataagt
    14461 aaaaatccct ataaaaatca aaaggggaat cataaaaata ccctataaca aatttaaggg
    14521 aatgtttgaa ccttccggaa ttatagtacc tttcaaacac ctcttcaatt ccttttaatt
    14581 cggacatttc actaaaactt atgtatttat tgttcaatac ttcatatgga ggatattctc
    14641 taaatttgta tccatgcttt tcagagtaat ttcttatatg ggagccttta agcattttga
    14701 gaaatcctaa ttggagttga tggggctcca gtgcgtaaac cctgttgaaa gattcctgaa
    14761 aggagatata gttttcaaag gggaggcctg caattaagtc caggtgaata tgaatattat
    14821 cctctgattt aagcagtctg atattatcaa atactttttc ggtatcagtt cttcggttta
    14881 tttcatcaag agtttcttca ttggtagttt ggacacctat ctcaagctgt ataacccctt
    14941 cactggcagt tgacaaaagt tgtatcattt catcatcaaa aaggtcgccg gcagcttcaa
    15001 aatgaaagtt agtttcatag ttggactctt tgctttgttc aatcacgaac ctgattattt
    15061 cttttgcccg cttcttgttg caattaaatg tcctatctac aaattttact tgttttacac
    15121 ctttgccaat caatctaaga atgtcttttt ttactctatc catagataaa tatcttacac
    15181 catcaaaggt tgaggaaata cagtaggaac aggaaaaagg acagcctctg gaagcttcat
    15241 aataaactat tttgttattt agtgaaccaa gcatctcatc tgtgtaagga gaaggtatcg
    15301 agtccaagtc tttaattaaa cagtatttgc catcatatat gatttttttg tatttttcaa
    15361 catcctcttc actgctctga ttgcagtttt ctccgttatt tctgtaacaa agcccttcta
    15421 cctccccaat atttaaatta tcagcagcca tggcggcagg gaaagtacta ctgttagtgg
    15481 cggaatacaa acatgaaagc agcttttgga agctttcctc accctcacca caaataatat
    15541 aatctataaa gtcgtttttt cccataatct cctgtggttc ataggatact tcaggtcccc
    15601 cgagaattac tataatatta ggtaatacct ttttcaggtt tcccgcaatt ttaagaatat
    15661 gagttatatt ccatatataa catgagaacg ctgcaacatc aggcttctca agaaatatct
    15721 tcgataatat ccactcaata tcatcattta tactgtattc tagaattttt atttcttcac
    15781 ggcaattact acaagcatat ttaagatacc agggtgcaag agcggaatgt atatattttg
    15841 agttaagtgc aacaattaca gttttcaagc cggcctccat aaattctgtt ttaacgggcg
    15901 gaagaatatc cgtctattat attatcgtta tatttttatc gtatatattc tcgtccatta
    15961 tatatcatca atcggtcagc ttcaaccact gctttataat cagttcaata taatatctaa
    16021 aatcttttct ctgcacatcg gcccatgttt ttagcacaca ttcagctaaa actttttcat
    16081 ccagcataaa ctttatctgc ccaacatcca taccggcata aacaggtgcc tccagacttt
    16141 catgaagcag aacttttttc tcaaggcgtt caagttcatc atgtcttaaa ggcaaaacaa
    16201 ctttttctgc agtgcccact ttcacactgt cttcaagtcc cttaacaaca ggcaaacttg
    16261 ctataatttc accggcttcc tgaagtgtgt aattagtata gttattgaaa gcatagtcaa
    16321 gtatcttttt actgctaatg gctctcacag acctggaagg acaattcaaa actacagata
    16381 caagtcgtct gccatcacgg ttaactgatg taacaaggca tcttcctgcc tgacctgtat
    16441 agccagtttt aacaccatta gttccgggat acatatccag catttcgtta gtattataaa
    16501 aactcctatt accgccgata gttatacttt tcgttccaac tatcttggag aataccggat
    16561 tattaagagc atatgctgtc ataatagcaa gatcataggc tgttgaataa tgtccttcct
    16621 tatcaagccc atgaggagat ttgaagcttg tattccttgc acctatcaat cttgcttttt
    16681 ggttcatcat ttcaagaaaa ttttccagag aacctcctac atgctcagct attgcaatag
    16741 ctgcatcatt gcctgaactg agcataaggc cataaagcaa atcctgatgc ttgatttttt
    16801 ctccctcgta aagatcgata acagagcccc atatcccact tgccagctta cttacagtaa
    16861 cttcgtcatc aagatttcca ttctcgatag ctactattgc tgtcattatt ttagtggtgc
    16921 ttgccatggg catcctctta taggcgtttt tttcaaagag cacccttccc gtatccaaat
    16981 ccataacaac agctgaaaga gcattaataa cagggggctg ttccgctggt gttgtacaat
    17041 cctctgtttc aaagatgttg gattgtattg tatcttgaca atattcatca gccaaagcta
    17101 ccctgggagc aagtatgaca ggcagtagag tttttccttc atgtatccga tcaggtaaag
    17161 gtagaaacat cccgataaaa ttaaatatta gtaaaatagt taaaacaata aaaatctttc
    17221 tggttgattt taagtttttc aattttgcct cctttaattt tggatccatt aagttcacat
    17281 aatagttatt gataatgcat tgggcatata gcaattttat atatttatat taatattttg
    17341 tatgtaatta tgcatataca tcaaaaaatt ggaggttaaa ttccaatatt tttttactat
    17401 aataaatatg cacctatcaa aataaggctg tccttttata ggacagccag gtgtaaattt
    17461 tttaatttga ataacactta acttgagtaa tgcttgattt agtttagcat atacttccaa
    17521 tatacttcca atctacttct gtgaaaaacc accacatctg cattcatcct gggctctaca
    17581 tgcagaatta ttgacactac actgtccttt taaagaaccc atcctggggg acagttcatt
    17641 acttgcttcc attctgcttg atgttttaaa gtttatacag tctttacatc tcatataaaa
    17701 acctccaccc atcaaatagg ttttacatca tcatatttga ttgcatctga ttcatttcct
    17761 gctgtgcctg ggaaatctgc tgctggctgg cagcttgtgt tttataccag cctttctgtg
    17821 acattatatc aaataactgt ttctgatgtt gaaatgtctt gtttaatata ttagtcgcgt
    17881 catttctaag gttctggttt gcactttcca gtacaagggt tgtcagagat gatgcactta
    17941 atttatgttg atccaatata acatttgtaa tttccttatc tgttatgttt ttcatcatca
    18001 ttcctcctta ttgcatagtt gttgtattaa tgtgcttcat taatacctga atatcgtttt
    18061 gatgatcttt tatcatttgc tgacatatac ccttaacctg gggatctgcc gtcatttcca
    18121 aacatccttc catgaatttt acagtgtttt gtgtcatctt tatattgtct tggattaaaa
    18181 gcagttcttt cgtagttaaa gccataatca tcctcctttt tctaataatt tgtagttaca
    18241 ttctgtaatt atattctgca atatttcaca aaatattcat aattccaagt ataaattcaa
    18301 aatgtataag aactctgcaa tacggtaaaa taaaatcgac tatcatgtaa tgaaatatta
    18361 gaaagggtga attttatgct aattgttttt gtcaggacat taatattata ttttattgtc
    18421 gttgttgtaa tgagaataat gggcaagaga caaattggtc agctgcaacc ttttgagctt
    18481 gttgttgcaa ttatgatatc ggaactggct gcagtaccaa tgcaagatac agcaattcct
    18541 ctgcttaatg ggattatacc aatattaaca ttacttattg cacagatgct attatctttt
    18601 atttccatga aaagcattaa agcaagagga atcatttgcg gaacacccag tgtattaatt
    18661 gaaaatggaa aaatcagaga ggagaacctc agaaaagaaa tgtatacttt aaatgatttg
    18721 cttgaacagt tgcgaacggt taatatacca aatatagcag atgtagaatt tgccatactt
    18781 gaaaccaacg gccagttaag tgtaattccc aagtcccaga aaaggcctgt aactgctgaa
    18841 gacctgagca ttccaaccaa atatgaaggt ttacctctgg atttaatcat agacggaagg
    18901 gtaataaaca aaaatcttga taaggcaaat cttgacgaaa aatggcttgg aaatcaattg
    18961 catgtccatg gtgttcataa tattgataat gtattatttg caagtctgga tactagtggt
    19021 aatttatact ttcaagaaaa aatgccaaca aattagctgc tgcaaggccc caaggaatgt
    19081 aatttttaat aatttgcatt atttatgtta tcttcaggag gtaaaaaaat aactatgaat
    19141 gattttataa aggttgtatt aatagtatca atacttctta caagtattat tggttcagga
    19201 atatatacaa atagtatttt ggcccgtgat tctaatttga tggaagaacg tataactcaa
    19261 atggaaaatc atctcaaagg aggcgattgg caaagcgctg agaaggaact ggtatatgta
    19321 aaagagtatt ggtctaaaaa gcaaggtaac tgggcagttc tgcaaagtca ttttgaaatt
    19381 gataatatag atgcatcctt tacaaaggtg acagaattta taaattcgaa agaactgtct
    19441 ctggcacttg ctgaatcagc tttgttaaag cagtttataa tgcatatacc taaaaatgtg
    19501 tcatttagcc ttgagaatat attataacaa catattgtga taatacatta tgataatgta
    19561 ttatgaccgc tgccgtacag attcatatat taatacaccg gcagcaacgg aggcgttaag
    19621 ggattcagca ctccccggca ttggtatttt tataaaaata tccgctaatg aagcagtttc
    19681 gttactaata ccatttgcct cattccctat tataattgca gcattatttg acatattaac
    19741 attaaaataa cttactgtgc cattaaggtg agcagcataa gtctttatac cttgagatct
    19801 aaagtatttt aatacatctg ccaaattatc acaaaaaact acaggtatat gaaatattga
    19861 gcccatagtt gagcgaagaa cttttgggtt ataaaaatct acacaccctt ttgatacaat
    19921 aatagctgta aaatcagccg catcagcagt tctgattatt gttcccatat tcccgggatc
    19981 ttgtatggaa tcaaggacaa caaatagatt gtctttatta ttttttaatg caataatatc
    20041 atccatagta tagtttctca tcttaacaac agcaagaatt ccctgcggag ttccagtatc
    20101 tgacattccc ttaaataagt tatccggaac aacatatata tcatactcta taggctcttc
    20161 ctttgatgta ataagtttaa taccggaaat tttaccattt atctccaacg tttcggatat
    20221 gaagattttt gttatatcag cacctgataa taaagcttct tccacgaatt ttaagccttc
    20281 tataaaatac agcttctttt gctccctgta ttttttagtt tttaatgctt ttacttcctt
    20341 aattaagggg ttgttttcac ttttgataag ctgcatataa acatcattcc aacctttctt
    20401 gagcaatttc tataaccaat ttatgtaact gattttatga tatatttctt atgcaggcat
    20461 ttaattatgc aattaacttc cctataaact caactacccc cggtttatgt ataaagacgg
    20521 gggtagtgct gtttatatta tcctatttat taaaaagcat cagattttaa atcctgattt
    20581 ctagttttgt gcagtcttaa cagtttcaac cagcttggca aatgcctgtg aatcatttac
    20641 agccatttct gcaagcatct ttctattaag atttattcct gctttcttta atccattcat
    20701 aaatctgctg taattcatac catttatcct ggcagcagca tttatcctag taatccaaag
    20761 ctttctgaaa tctcttttct ttgctcttct atctctataa gcatatacct gtgatttcat
    20821 cactgcttga ttagctattc tatagttttt acctttttca ccaaaatatc ctttagcaag
    20881 ctttaaaacc tttttatgtc ttgcacgggt tgttaccccg ccttttactc tagccataat
    20941 tatttaccct cctacttata tggtatcatt tgtttaactt ttgaataatc tgcacttgaa
    21001 acagtagtac cttttctcag gtttctcttt ctcttagtgg tttttttagt cagaatatgg
    21061 cttttatagg ctttaaacct tttaactttc cctgttcctg taaaattaaa ccgctttttt
    21121 gaagaactat gagtcttaat cttaggcata attatcttct cctccttaac attaacatat
    21181 gaactaaaca ccctctgcat tttagtacaa aggatggatt tttcttaaga atcagcctac
    21241 tttggattta aaattataaa catactcttt ccttccagct ttggcctttt ctctgtcacg
    21301 ccatactctt taacagcttc tgcaaactta tcaagtattt cctgccctat tgaggtgtaa
    21361 tttatttctc ttcctctgaa cctcaccata actttaacct tatccccatc tttaagaaat
    21421 ttaatggtat ttttaacttt aaagttaaaa tcattatctt caatattggg actaatacga
    21481 atctccttga cagtgataac cttctggttt ttcctggctt ctttttcttt ttttgacaac
    21541 tcaaacatat atttaccata atccatgatt ctgcaaactg gcggaacagc tttcggcgct
    21601 attttcacca gatccaaatt tttggcatta gccagcttct gagcctcttt agctgacata
    21661 acaccaagca tattcccatc aacatcaatt agtcgtattt ctttatccct tatctcttca
    21721 tttaccatta attgatcctt taaaataatt aaacacctcc atataaattt tactttaatc
    21781 cttcagcagg aagtctgtga ccccttgcat aagccattaa tttgtgtagg tttggcatat
    21841 aaccatagta ccttttgatt acttggtcat cgaccaccaa tcttgcctta aaaacacaaa
    21901 aataaagtgg attgagaaaa tcaacccact gtatatccat tcaagaattt tgctgctaat
    21961 atttaaccgg cattacttca tttattaaaa agcatctaaa caagctaaac aagcatggga
    22021 aaccataata atttgctgta ttatatgcta atgcctgcag ttaattctct attaacccta
    22081 tcagataaac tatctgtaag gtgagaagtg gttgacttct tctttatttt tgcttctata
    22141 tcatagcaca agtaattatt gttgtcaaat atttttttga gtcggggcgc attttgtatc
    22201 atagtaataa aatgcgccca cgataattac ttacagtgct tttccattaa cctcttgagt
    22261 tattttgctg ataaaattgt ccaagagcat agctccaaga tctccgtcct ttctggacct
    22321 tactgcaact gcgccatttt ccatttcctt atcaccaatt accagcataa atggtacttt
    22381 ttcaagctgt gcctctctta tcttgtatcc gattttctcg tttcttaaat caatttcaac
    22441 tcttatgcct atttcttcaa gtttctgttg tacactacga gcataatcgt aatgcttatc
    22501 tactatcggc agtactttaa cctgtactgg tgaaagccat acggggaatg ctcctgcaaa
    22561 atgctctgtg agtattgcta taaatctttc aatactacca aacacaactc tgtgtatcat
    22621 aacaggtctg tgtttttctc catcagcacc aatataagtc agatcaaatc tttcaggcat
    22681 ctggaaatca agctgtattg ttccgcactg ccatgtacgt cctatggaat cctcaagatg
    22741 gaagtctatt tttggtccat agaaagcacc atcaccttca ttgattttaa atttcattcc
    22801 aatggattcc attgcatctc gcaatgcatt agtagccatc tcccaatctt catctgagcc
    22861 catggaattt tccggccttg tagagagttc tacatggtat ttgaagccaa atactttgta
    22921 aaaatcatca atgagttcaa taactccctg cacctcgtct ttaatctgtt caggcgtcat
    22981 gaatatatga gcatcatcct gagtaaagca cctaactctc attaaaccat gcagagcacc
    23041 tgaaagctca tgtctgtgaa ccagtcccat ttcagccatt ctctggggta gatccctgta
    23101 tgaatgaagc tttcttttat atacaagcat gcctccggga caattcatag gctttattgc
    23161 ataatttcca tcatcaattt ttgtaaaata catattctcc ttgtagtgat cccaatgtcc
    23221 ggaacgatgc cacaaatcct cattcagaat aatcggagtc cttatctcct ggtatcccct
    23281 tttcttatgc tcttcccgcc agtatccttc caggaggttt ctcagtacca tacctttagg
    23341 cataaagaaa ggaaatccag gaccttcatc cattatatca aaaagatcaa gttctcttcc
    23401 aagttttcta tgatccctct ttttagcttc ttccagtctg aataaatact cgtcaagttc
    23461 gcttttttta gggaaggaag ttccatatat tctttgaagc atcttgttct tttcgcttcc
    23521 cctccaataa gcgcctgcaa cggataaaag cttgaaggct tttattttac ctgtagatgg
    23581 caagtgaggt cctgcacaaa gatcagtaaa atcaccctgt ttatagaaag atatttcctc
    23641 cccctcaggc aaatccttta ttaattcaac tttgtatggc tcaccttttt cttccatgaa
    23701 tttaacagct tcatctcttg gaagagtaaa tctttcgaga ggcaggtctt ccttaattat
    23761 tttcttcatt tcctcttcta ttctttcaag ttcttcaaca gaaaaaggct tatccttgtc
    23821 aaaatcataa tagaaacctg tatctatagc agggcctata gctaatttaa catcaggata
    23881 taatctccta acagcctgtg ccattatatg agagctggta tgcctgtaag catcttttcc
    23941 tccctcatct tcaaaagtca gcaaactcag cctgcagtcc ttctcaagct tgtagctcaa
    24001 atctttgacc tcaccgtcaa cctcccctgc cagtgcaacc ctggcaagac ctgcacttat
    24061 acttactgca acttccttaa tagtaatacc tttgttatac tcattaacac ttccatcttt
    24121 aagtgtaact tgtatcatta aaatcacctt cctttaatgg ttctatcttt tttttctcgt
    24181 gttatacctg catgttatac tttaatgtta cactagcatg ttatagctgc acattatagc
    24241 tgccgtatac ttataattac actttaacat caaaaaagct cccatccctt gcatatacaa
    24301 gggacgagag cattctcccg tggttccacc ctactttgaa ctcattacga ttataacgga
    24361 atcaccattc caactcagag acggtcttca actgcttttc cctgaagatg cttccagcat
    24421 gctgcacctt ctctctggag ggaacgggac aatttactat tctcatcaat gtcggatatc
    24481 tatattagct ttatttctat agcatgtagc actattcttt aatttattcg taattcctat
    24541 ttaatgtgca ttagttttcc aaatgcatta atttttatag gatattcgtt atttttaatt
    24601 atagctgtga gcggtatatg tgtcaataaa aaagttttaa aagttaaaaa gttttaaaaa
    24661 ttaaagtatg tgcactctta gaaacttaaa aatattaatt atgtgctcat atggccagta
    24721 tctttatctt ccagtatctt tatcttttat ttttttgtta taataatcca ttgctttcat
    24781 acttaactct tccaagctca taccctcaaa taaatttgcc tgccattttg tataatcgaa
    24841 gggttctctg ataattaaag atataaacct ctctgcatca acttttccca attttttgag
    24901 tagtacattc attccttcct gcattataac agaatccggt cttctggcta tattttccat
    24961 caatcatccc tccatttttg aaataaactc taaaggactt aatacctgta tttctggtat
    25021 taatgtattc aataacttct tatccgtagt taaaaacaca ttacatttcg cttccacagc
    25081 acatgcaata tgtagtgcat cctttttctt aagtcctttc tccaaaagta atttcgcaaa
    25141 gttgctaatg ttatcgttta atattatata tgacttcgca acgcttctcc aacctgcaat
    25201 tgagttcttt ctcagttcaa agggattttg ctcattttca aaatccaata taaatgacca
    25261 cacaagttca tattctcctg aaataatgtc tttttggata tccaatttcg cttgcgtttc
    25321 caaatagatt tttaactgtg tttggtcatc ataaggtcta ttgaagcaac agttatctaa
    25381 atatatcttc atctttcttg tacgcacctt cttgcaatat tatttggtgt atttatatta
    25441 tagcaacaat cgacttgttt gtatacgaat agtttttatt tgtttggaaa tataaaaaat
    25501 tttaggagta acaaaaaatc ttgattttca ctacaacttt ttgacaaaaa cgaaaagccc
    25561 aaaactcttt atttaaaagc tgttgagggc tatttctatc ttattttgct gcaaaagtta
    25621 tgttatttta aaaaatagct gtgagtgata tatgtgtcaa taaaaaagtt tttaaatccg
    25681 tacacttaac tataattcct tttctactcc ccaggaaaat tgtgcagtgg ctccaagtcc
    25741 catatgaaac attacaggat ggtaattata ccatgctgtt gtaaccaatc cttcagcgtt
    25801 tgcttcgata cacctttcag cagccgtcct tatattcggt ataaacctcc agtagttggg
    25861 caaagtattg taatcatctc cgttgccaag actggtaggt gctcctatta ctttaaaccc
    25921 tttatcctgg aaaaattcca ggtatggata accttttatt ctctttggaa actcatttcc
    25981 aagatatggc ccatataact caatgaattt tttactcagg ctttccttca aaggtacctt
    26041 gctcgcgaaa gtactcatga ttgacctttc aagatccgtc aattcatgtt tccactcatt
    26101 gtcccaacct gcatcacaca ctgtgacagg tcttcctttt ctatcatacc ttgcaataaa
    26161 ataagggctt gatttgctta ctgtccagta atcccagtac attataacaa ttcttttgtc
    26221 taataaatca agggcttccg ggtgtgcaca gagcacatca tcccaaataa ttggagttat
    26281 tccttttgat gtaataaaat cacaaattga atttatgtga tctatgtata gtctgcttat
    26341 accatacttc tctacctttt ctttgcaaga aggacattta ccaagacttc tggcttcatc
    26401 cccgccaatg tgtatgtatc tgatacccgg atgtgcttct atcatctctt ccagcaattc
    26461 tttaataagc tcaaacgaat ccggattaag aggacatatc tggcttatag tctctctatt
    26521 ttccctaatt gatttgtagt aatcatgttc taatatgtat tccaaatgcc ctattgtttg
    26581 ctgcaaaggt attagttcaa tcccaagctc ttttgcttta tcgttaattc tttttatatc
    26641 ttccttttta agagttgtat taacagctat tctactatgt ttctcgtacg gaaacctgtc
    26701 ggcatattca agtaagattg tgtttagctt aagttccgca gctgtttcaa ttgagcgcaa
    26761 tgcttcttct atatccattt gcctgaaact atcaaaatta atatgaaaac ctctaatttt
    26821 tattgaaggt ttatcttcta tatttcctgc agggatatta ccgtccggcg aaagctgcca
    26881 taaagtttgc attgcataat caatgccctt ttccgaacca gctgatatat ttacctgttc
    26941 aggtcctata ttaatcttat acccttctga attcatatta ttatccgtat gtaatgaaat
    27001 tttcccgatg caggatccgt ttaatcctgt ttcaccacca tggcttacac ctgccattcc
    27061 aaactttctg gaccattttt cagcaatatt ggttatactg ctgcatctct gctgaattcc
    27121 atcagtttta caaaccttgc ctgattcctt gtccaattca atgtaccaat tatcatttaa
    27181 ctttatgaaa tcgccattaa aaactgcctt tttaggtaat ggcaccaccc ttttatgctt
    27241 atcagctatg tttttagtaa tcatttgcaa acctcgcttt cttttgtttg ttaattatta
    27301 ctattataag atttgtttct acatcagcaa agactatatt tgctcttaac tgctgtttct
    27361 ctgtctatcc atcttccatt tgtaaagtca gggaaggcta ccgatgaact gcctctatta
    27421 atagactctt ccgataaaac agttattgcc atccaagtgg cggtatcata gacatcaatt
    27481 ggggtttggg ttttattcct aatactctca gcaaaagctt ttaagagtaa gtagtccata
    27541 cctccatggc cgccttttac accactttct tcgaattcct tccaaagcgg atgaccatat
    27601 tcttcataat aatcatctat tgattcccat ttttctcctt cagtttttcc atctataaat
    27661 attcctttgt tatcctccat gtaaattcca tttgttccat gtacgacatt gaaacgagaa
    27721 taaggtctgg gtaatgttac attgtgagtt acaagtactg tttcgccatg tgcacatttc
    27781 atcatggttt gaactacatc accttttacg aaatcgaagt ttgctagttc ataatcaggt
    27841 tctttatgtt tttttatcca atctcttaaa ccaactgctt ttgatgccat ggatgttaag
    27901 gaaataaaac gattacctct gttaatgttt aacattttag caattggttc aactccatgg
    27961 gtcggatata aatctccatt cctatgcatg ttattgtaaa gtcgattatt tggacgtcca
    28021 gccattgtgc gtatatcatg accataacca cattcacaat gtactatttc cccaaaaagt
    28081 ccttttttta ccatatttaa tgtcattagc tcgtatttac catagcagca gttttccagc
    28141 atcatacatg gtatcccggt ttcctcggat actcttacaa gctgccagca ttcttcaata
    28201 gaggctgctc ccccaacttc taagccaaca tatttcccgg ctttcattgc agctattgat
    28261 aaaggaatat gtgttatcca atttgttgct ataactacac cttcaatatc tttccttgca
    28321 aggagttcat tgtagtcagt gtaagtatcc ggtctttgca aatcagcatc atcaaagtac
    28381 ttgaaaattg tctcaaaatg agcaaaatca gcatcacata tagctgtaat ttgaatttct
    28441 tcaatatgta caagaattcc cattaatgtt cgccctctag gcccaaggcc gattaagcct
    28501 aatttaacac ctttctcttc tattgtaatt ccacctttca tattagttag tgctatacaa
    28561 tttcaagttt ttattaaatc tagcaatcat ttgacacttt tattttgtca aaatatgcta
    28621 taccttggct aggtgtattt gtctaaaata gagccatacc tacttcaatt gttcattaat
    28681 ctctattggt cttttttctg caatagaaat attggcagat gcaccaatta acagagacat
    28741 tgctccatcc attgagctgg cctggcatcc taatgggtcg tctacatcgc ctataaaaag
    28801 catcctcatc agtttgtcat ctcctccacc atgatctcca actgccttag gtatatcata
    28861 tataataata tcaccatttc tattaaagaa ctttatttgt ctatatggtt ggtcagcacc
    28921 cttccctcta aatatttcgc ctatttccag tctgccctta gttccgttaa tggcagctct
    28981 atatccttca tatggactgt gagcaataag tgaataactt aacaatgtac cttttgagta
    29041 tttaacattt actgacattg tatcatatat gttgatatct tctgcaaata cacaagcatc
    29101 tcttatatac ccatcttctt tttcagcatt caagaaatac ctgatataga acgaattgtg
    29161 tgcactgcta ttaatattcc aataatgctc gcaagttttt ttatggttgc aagctaaaca
    29221 tctttcacct ctgttttctc ttgttggtcc ataatatctt cttgttccga atgcaaaaac
    29281 ctcctcggga tattgatcaa tccaccagtt tataaggtca aaatgatgtg tagatttatg
    29341 cacaagtaaa ccaccgcttt tttcaatata tctatgccac cttctaaagt attgtggtcc
    29401 atgaatagta tcaaccatcc attctaggtc tacatttaat acttctccaa ctattccctc
    29461 tttaataagt tcctttactt tgcttgcata tggcataaaa cgcatattaa aaataactga
    29521 tattttgttg cctgttcttt tttccgcttc taatattgcc ttgcatttct ctgaatctat
    29581 tgtcataggt ttttctgata ttacatcaca acctgcttct agtgctttta taatatattc
    29641 atgatggtaa gagtctattg tagtcacaat aacaacatca ggcttagtct catttatcat
    29701 ggtgtcaaaa tcattaaaaa ctagaatatc accgcagtct ttactaaatt catttgctct
    29761 tacactattt atatcatata cacctaccaa acttgcataa cctttaaact tgtcagatga
    29821 tagccgagtt ccgaacatat gtaatcctct atctccagca cctataataa catatttttt
    29881 catgtaaaaa cctccctttt ttgtcattac aaaaccttat gtgatttgta actaaagagc
    29941 agttatacta atataaatgt aagtacaact gctctaaaaa atcaaatcag aaaagagcta
    30001 agtggtcatt ttgattttta acataacgct catatgcagt ctgttgaatt tgtaaaaatt
    30061 catccaggcc cattttcttt aatgtagcaa tattattatc ccattctgta aatggtgttc
    30121 ttccagttag aaaatcagct gttgtttttg caacatatga attaatatca gaagataatt
    30181 ctcgcactct ttcattttct tctatagtca agtcaaattg tgtccaaact tcttttaaac
    30241 tatatttttg cattttttcc gctgcgttca atggaattgg ctgggcttct gcacccttaa
    30301 acacattttt caatagtaca atcggattat ccccaacaga accaggtgtg tacttatttg
    30361 ttgcttgacc atagtctagc ccatcaggat tttttatatg tgtaacacct tcttttccta
    30421 gaaaataaag tgcactacct tcttcagtat aaaaataatc aacccatttt acagtttcaa
    30481 taggatactt attttcattt gtaattacaa atgaaccagt tgttaaagct gaggggttgg
    30541 cattccagtt t
//
LOCUS       DAOSDD010000023        29032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_761_length_29032_cov_5.210374, whole genome shotgun sequence.
ACCESSION   DAOSDD010000023 DAOSDD010000000
VERSION     DAOSDD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 29032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29032
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_29032_cov_5.210374"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK733_03740"
     CDS             <1..469
                     /locus_tag="PK733_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09688.1"
                     /translation="LGGTRVPLLQKANLFPVIYVGSNIWKNLGWSSIIYLSQLSSIDP
                     QLYEAATIDGAGRFRKMWSITIPGILPTIIILLILRIGRMLSIGFEKIILLYNPLTYE
                     TADVISTFVYRKGLMDFAWSYSTAVGQFNSVVNLLLLVLANKISKKVNEISLW"
     gene            479..1357
                     /locus_tag="PK733_03745"
     CDS             479..1357
                     /locus_tag="PK733_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09689.1"
                     /translation="MNRKLKTDYIFDTINYLFMFILILVTLYPLLHVFLGSISDPYRL
                     QKHRGILLYPLGFDLESYARVFANKSIVTGFRNSVFITITGVLLSMIMTILAAFFLSR
                     RDILLKKHVMWFIVLTMYFSGGLIPSYMNIKELGLYNSIWVLIIPGALSTWNVILMRT
                     YFLGLPNELEEAATIDGASEYYTLTRIIIPLSKPIIAVLVLYYGVDKWNEWFAASIYL
                     RDRKMYPIQLILREILINNDPEKMMEDVMLTDKSAYRETIKYATTMAVTLPILFVYPF
                     LQKYFVKGVLLGSLKG"
     gene            1462..3789
                     /locus_tag="PK733_03750"
     CDS             1462..3789
                     /locus_tag="PK733_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09690.1"
                     /translation="MKKQVIQNRGVLFKWLLSYMVVLALPLTTSYFLNKVSTDALNKE
                     IHLVNANVLTQIRSSIDEKLKEMEKLALFLGSDSNLLGLVEKNADKKMTFHYDLHKLV
                     SDFKSLLAANDSISDFYVVFHNADIVVTPGAVLNHEQAYSILHKNQEMSYVQWKQQVM
                     KPYIRGYVTCSRRLSTSSEVNTRVIAYMHSLISNDLQNAAATVVVMTDERQLQPYLSI
                     QGELQDSEIYLLNANNEVMLSSTKSTDLPVQLNFDDLNEGVWKNITTKGIELAVINNK
                     SSITNYKYVGFMEYSQNWKRLMDMRKLSLCLILAGFIGGFLAACWFTWRKYVRISDLL
                     QFIVKNLKIKKPKQDDEFVFIRNAMNLTINEKENLKQEYDQQKGILFRTFLNRLLRGE
                     ISPLTMTEDTIRFYGFQDVTPNWMVVLFHFDMKKSSGYEEYSLIRKKDILSEIKEILN
                     QNIPQGYGIYITEIDDMIAALVNTNDRNPGKLQNICRKAGVVLKEMAICGITIYSAVS
                     SIHGKSLQGIATCYTEALEVLEYSIMFGVNTVMSYNQLGKHYQTFEYSIDMEQKLINN
                     IRSGNADSTRSLLYEVFEKNISKNKIGLDMLKILMLNLVSTAIKAADSNTVSKLMEDI
                     QPVRCIDSCQTIPEVKQQMTSILLYVCESCKENEKTRNEKLCSDVMDYIKENYTNPEL
                     SVTYISEKFEFSPIYLSNVFNKCAGVSIMDYAHRCRVEMACEYLNNSYKSAKEIAALV
                     GYIDINTFSRNFKKCKGITPGEYRKIGPELENQLS"
     gene            3960..5546
                     /locus_tag="PK733_03755"
     CDS             3960..5546
                     /locus_tag="PK733_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09691.1"
                     /translation="MKSFTIHKDSIIGSNTGFWKAAGSDLLFWSALRPEGQDLLNRMM
                     KTGSCRYFRCHYTFSKRIEYNAQVGGEVYSEDEKGNPCYSFEKINRVFEEYLKHGIKP
                     IVECDYLPDELNIEKVSGYLDEGLANSTKGPNNWEKWYNLIKNFVKNLIDRFGAEEVR
                     SWYFEVWNEPDNFPIEELSTFFKMYDVFVDAVTSIDNQLKVGGPACFTPAFLTHFLNH
                     VANGTNYVTGKTGTRIDFISYHIYGMSGKWIGEYPLVQPTVQRFIHEILWVQRIIKRF
                     PRLENVEFHLNEWGVCSNFYLTSDEYPALEFRNSEFSALFLVKLVHSLYALCDIFKFP
                     ISVLLYWGFAYEAAGNRLFHGMRDLVTVGNIPKPIQTAYEMLAMLGEHRLKVAGGKPG
                     GNIGLLATVTGDFEIQFIIYNFCELHEFNLKEHNDRIEDRDGAEEIEIIIENLPGGKY
                     MEVTSYYMDSEHHNTYRIWQNLGAPPFASNTNINILKESGELKPNYFERVPINSGSAK
                     LRFRLQGNSMRLYLGKAVYK"
     gene            5678..7096
                     /locus_tag="PK733_03760"
     CDS             5678..7096
                     /locus_tag="PK733_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09692.1"
                     /translation="MNSNQIIEKVCKAIDALITSGEKYNGLFPSMIDRDSNEMIMEFT
                     PLNDGQREHDRTFLGSNLMHDHVVLKTIYAIGNVLNKKNYIEAADKYLKYFVSHCTNT
                     VTGLFPWGEHAYWHLLEDKAAAGQDLKPDSTGGSKLVHDHLNQAPLWLWEKLYEFNPN
                     VVIRFAKGLDMHWKSKHPYEYNRHAYINIKEPFKMKKTYWADFPRHGGFYIFDWAFAY
                     RKTRDKEFYDQIITMLDYIPASKDKNGLLSYATNSIGRDELLKAPAQTLSLAASMLEA
                     AEILYESNLELSIRLQKEAGEYLNGFFSVPHDNDEDVYVLSCRPETYEITGTMPIWSS
                     KYGVPPAAYSGLICNCCYRITKDKRLLDYAKSVAISYVKKDFPDSLGIPALVCGLVLG
                     LFADLYEISGEQNWLSEGLKLADIAIKVYLDKDLPRCATGIDIYESQMLPGFLLNGLA
                     RIALLSENRINCGIEPDYSYRQ"
     gene            complement(7232..8800)
                     /locus_tag="PK733_03765"
     CDS             complement(7232..8800)
                     /locus_tag="PK733_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ09693.1"
                     /translation="MERMVLIPDMNNTTSNGFAKPPESYWISSIPQTNFPHLEEDVKV
                     DVVIVGGGMTGITSAFLLKEEGLTVAVIEADQILHGTTGHTTAKVTSQHDLIYYKIKS
                     QMGQEMAKMYADANETAINTIASLIERKKIDCDFYRSSAYVYTQDDKYIQQILDETNT
                     AASLGIQAFYLVETPLPFSVKAAVRFDNQARFHPLKYLIALANEIPGNGSYIFEHTRA
                     IDIHEDKYCHVVTNSGKRVTADKVVVASHFPFYDGYGYYFSRLFPYRSYIVAVKIKGE
                     FPEGMYISAEKPVHSLRYQNTDDGGVVLIAGENHKTGQGKDTSVHYENLKNFAQKIFE
                     VEDIPFRWSTQDYTSADEVPYTGHLSSKTPNIYVATGFRKWGMTNSTASSIILRDLII
                     GRENPWKELYNPSRFTPGASAKNFIVENADVAKHLVVRKLAAVPDSDDVDLNPGEGRV
                     VEMGGQKMGAYKDEQGRLHLVDTTCTHMGCELQWNSAEKSWDCPCHGSRFTYDGDIIE
                     GPAQRRIKKEIQEQ"
     gene            9045..10046
                     /locus_tag="PK733_03770"
     CDS             9045..10046
                     /locus_tag="PK733_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09694.1"
                     /translation="MEENMEHVDKSIALPANLDFEIEKAIQMRETFNKLFNNLLQQGV
                     DFDRIPGTDKPTLLKPGADLLCQVFHFASGEPKMLSIIEDFEKGILSYTVSIPVIHRD
                     TGTLVAVGIGSANSFEVKYKYRYVDADGGEKVRVENPEPADQQNTLVKMAAKRAYIDG
                     VLKATGASRMFTQDIEDMPWLQPEKASSKQLNYIRALYKDVPDDKILEDAAGIINRNI
                     ENVDDISREEASRIIEVKKSKNYPQYPLYTQEQADTKSDLKYQAKKKTEAYSCANCGV
                     RITQGVAAYSRNKFGKLLCGNCQKSTDVFNPAQPDKDNYSGNFQRNETEHDSTLPPS"
     gene            10279..10818
                     /locus_tag="PK733_03775"
     CDS             10279..10818
                     /locus_tag="PK733_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ09695.1"
                     /translation="MKIGEKIKRLRLKNGLTQEELASRCELTKGFISQVERELTSPSI
                     ATLVDILESLGTNLKDFFNDIVEEKVVFKKDDVFEKQDSELEHTIKWLISNAQKNSME
                     PILMYLDAGGNSGEDDPHDGEEFGYVLSGSIHLYLGARKHRVKKGESFYFKPSIVHSI
                     SNAGKTEAIVLWVSSPPSF"
     gene            10840..11886
                     /locus_tag="PK733_03780"
     CDS             10840..11886
                     /locus_tag="PK733_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09696.1"
                     /translation="MPEPIIRLINISKDYNGAEALKNINLYIRKNEFLTLLGPSGCGK
                     TTTLRIIGGFEQPTVGDVMFEGKNITNLPPYKRRVNTVFQKYALFPHMDVAENIAFGL
                     RIKKLDEKVIKKKVAEMLELANLKGFERRQVDSLSGGQQQRVAIARALVNEPEVLLLD
                     EPLGALDLKLRKDMQIELKRIQQLLGITFIYVTHDQEEALTMSDTIVVMKDGTIQQIG
                     TPEMVYNEPENAFVADFIGESNIITGKMIKDRLAYFAGREFECVDAGFEENENVDVVV
                     RPEDIKLVREAEGMLHGEVESVTFKGVHYEMIIKSRDIRWMVHSTLMEPVGSRIGMLI
                     LPNDIHIMKKVGEK"
     gene            11883..12710
                     /locus_tag="PK733_03785"
     CDS             11883..12710
                     /locus_tag="PK733_03785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ09697.1"
                     /translation="MKRTWYSYPYLVWMVIFIVIPLLLLIFFTFTISTDEGIKFTMEH
                     IRRLWDPIYLMVFLRSAGLALISTVVCLLVGYPVALILASRDYRNKKAMAMLFVIPMW
                     VNFLLRTYAWMTLLEKNSLVNIILGYLHLPQLKLLYTNQAVVLGMVYNFLPFMVLPIY
                     SVLVKMDRNVIEAAEDLGADYLTILKKIIIPLSLPGVMSGITMVFMPAITTFVISRLL
                     GGGHYTLIGNLIEQQFIFAGDWNFGSALSFVLMIIILITMGITRKYDKEYEGGGAIW"
     gene            12704..13516
                     /locus_tag="PK733_03790"
     CDS             12704..13516
                     /locus_tag="PK733_03790"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ09698.1"
                     /translation="MVSRIAKKAYLYLVFIFLYAPIIVLVIYSFNESKSRGRWGGFTF
                     KWYGELLKDREIAAALYNTLLVALLSSIIATIIGTAAAIGIQNMKKIRKAIIMNLTYL
                     PVLNPDIVTGVSLMILFIFLKAFTQLGLGFFTMLLAHITFNIPYVILSVLPKLKQLNK
                     HMYEAALDLGATPGYALRKVIIPEIMPGIITGLLLAFTLSIDDFVISFFTTGSGVSNL
                     SIYIYSMARRGINPKINALSTLMFLTVLILLLIVNKRMSKDATSGNAKEVVR"
     gene            13513..14595
                     /locus_tag="PK733_03795"
     CDS             13513..14595
                     /locus_tag="PK733_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ09699.1"
                     /translation="MKKIKKSIVKSNEKSIVKSIAAVLIITAFAGLFAGCGKSNKAKL
                     KVYNWGDYIDESVIEEFEKKYEIDVVYDTFATNEDMYVKLKGGGSDYDVAFPSDYMIE
                     RMIKEGMLEKIDFNNIPNYKYIDERFKNLEYDPDNEYSVPYMWGTVGIIYNKTIVKEP
                     VDSWKILWDKKYSKQIFMLDSSRDSIGITLKMLGYPINTRDKSELEAAKNALIEQKPL
                     VLSYMGDDIKDAMIAGQAALAVVWSGDAVYMKRENTDLEYVIPKEGSNLWFDAMVILK
                     GGKNKKEAEMFINFLCESEIALKNTDYIGYSTPHTEAKKMLDPEVLNDRTAYPTDDEI
                     ENCEVFQALSDVIKDYDRIWTEVKAK"
     gene            complement(14687..>14956)
                     /locus_tag="PK733_03800"
     CDS             complement(14687..>14956)
                     /locus_tag="PK733_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09700.1"
                     /translation="EGRLDTVDSRLAAVEGRLGTVDSRLDALDSRLDTVEGRLDTVYE
                     QTAMLTEFRAEVNQKLQNMADNITSIVEITGEHEIQIRNIKRKVV"
     assembly_gap    14957..15056
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15412..16254)
                     /locus_tag="PK733_03805"
     CDS             complement(15412..16254)
                     /locus_tag="PK733_03805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ09701.1"
                     /translation="MDKRLYGKLGETLSIVGFGGIIVMNEFQEDANNFVAEAIDSGVN
                     YFDVAPSYGNAEERLGPALVGKRNNIFLACKTEKRSKVETEKALYESLERLKTDYFDL
                     YQLHSVSTIDDVKKVLGPNGALETLTKAREKGLIRHIGFSTHSEEAAEELINQFEFES
                     VLFPLNWVNMFNKGFGKRIIEKAQEKKITMLALKSMAKTLWKEGLEREYPKCWYEPID
                     DKELAQLAIRYTLSKPVTAAIPPGYIKFFRWALEVARDFKPLSAEEEQFLISRSVGFK
                     PIFQ"
     gene            16503..17060
                     /locus_tag="PK733_03810"
     CDS             16503..17060
                     /locus_tag="PK733_03810"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOJ09702.1"
                     /translation="MNNNFNLAADSSIIYKAYYRSPIGVISILGNNKGIVAVDFIDEE
                     ESTCDLIIKVSNEEKIPQLPRCIQECIRQFDEYFAGRLRNFTVDIVINRGTDFQKKVW
                     NTLREIPYGETSSYALVAEKIGNRKAVRAVGNANNRNPISIIIPCHRVIGSDGSLVGY
                     GGGLWRKKWLLEHERTNHVLKQITY"
     gene            17251..17985
                     /locus_tag="PK733_03815"
     CDS             17251..17985
                     /locus_tag="PK733_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ09703.1"
                     /translation="MKKILKNIAVLFLITTIVLAVAGCSNKGTLKMGTNAEFPPFEFV
                     EGGKFAGIDVELAEEIAKELGMKLKIENMAFESLIDAVSSGKVDFVAAGMTIRPDREE
                     NADFTMTYYNAIQTIIIKEDNSNIETKDDLEGKRIGVQTGTTGQYLAEEIKDAEVGKY
                     NNGLEAVMDLKNGKIDAVIIDNFPAKTYADKNPELKLIEGQFEEEQYAMAVKKGNTEL
                     LEKINNALQKLIDNGTYQKIVDKYSK"
     gene            18087..18779
                     /locus_tag="PK733_03820"
     CDS             18087..18779
                     /locus_tag="PK733_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ09704.1"
                     /translation="MVFIENLYRVLSDKIYYAFIKEDRFMYLVNGWSISVQLTLFAAI
                     LGIFIGMFIAIAKITGKPPFAQIADWYVSIIRGTPAVVQLTIIYFIILADVNLPKTVI
                     ASIAFGINSGAYVSEIIRSGIQAVDKGQTEAGRSLGLSYSQTMRHVIIPQAVKNILPA
                     LGNEFIILLKETSISGYIALDDLTRGGTLIRSVTYDSMTPLLTVAYIYFLMTAMLSAL
                     LAKFERRLRSSD"
     gene            18772..19494
                     /locus_tag="PK733_03825"
     CDS             18772..19494
                     /locus_tag="PK733_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ09705.1"
                     /translation="MIKVINLHKYFGDLEVLKGVNNEIKKGEVVCVIGPSGSGKSTFL
                     RCLNLLEVPTRGEVIIGDIALTGNMKDIDKLRRHVGMVFQQFNLFPHMTVIENIMLAP
                     VKVLKKSKEEAAAEALELLGRVGLADKANEYPSRLSGGQKQRVAIARAMAMNPEVMLF
                     DEPTSALDPEMVGEVLEVIKALANEGMTMVIVTHEMGFAREVSDRVLFMDEGIIYEEG
                     TPAQIFSNPQRSRTREFLSKVL"
     gene            19652..20317
                     /locus_tag="PK733_03830"
     CDS             19652..20317
                     /locus_tag="PK733_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09706.1"
                     /translation="MYIAKVIYIAIAVLTILPLIAFNIKRFTVKREGENPRPNIVIII
                     LTLGGSILLGVFLFSLYKFTLDYQPQLVLERFIRSYSSTAAGKIDYEKFLKETESICT
                     PEFLSLKDRCYREIEADRQAGLGTVSFQLGETILPKYYFDKEFFPNVEGIKQNDKQPI
                     YMLSMIEYGETRKFYITLLRIQGNKWYVDSFNEASKELAEYAYRYNFMKSEHANKWFK
                     VKK"
     gene            20538..21365
                     /locus_tag="PK733_03835"
     CDS             20538..21365
                     /locus_tag="PK733_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ09707.1"
                     /translation="MRKYLLKKIIVAVLSLVLSASMLLTGCGDSGSKADNSWDKVKEK
                     GEFVLGLDESFPPMGFKDDKGEIVGFDIDTAREVCSRLGVELKIQPISWDAKEQELNT
                     GNIDCIWNGFTITDERKENVLFTKPYMNNRQVLVVKEDSPYNDIKDLEGKKLGLQSES
                     SAVDALDANEDFKAKLKEVILFDENMMAIMDLEKGGVDALLIDEIVIRYYMQTKGNNF
                     KIFDKELSHEEYGVGFRKGDKKLMKKVEETLEAMSKDGKLTEISTKWFDRDIVTIKK"
     gene            21748..22452
                     /locus_tag="PK733_03840"
     CDS             21748..22452
                     /locus_tag="PK733_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ09708.1"
                     /translation="MLENINWNEIALVLEDFIKNFVRLCKLLGAGGLVSLKVFFLTLI
                     FSLPFGLLIAFGRMSEKKWINGPVRLFILIMRGTPLLLQLIFVYFTPSYLPEPIRINL
                     DRFTAAIIAFSFNYSAYFAEIYRGGIESISQGQYEAAAVLGFTKIQTFIRIILPQVIK
                     RILPSISNEVITLVKDTALVMSLGISELYRAARNEAARVFSTSPIFIAGLFYLLMNYV
                     VTKVFDKIEKKLSYYR"
     gene            22610..23362
                     /locus_tag="PK733_03845"
     CDS             22610..23362
                     /locus_tag="PK733_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ09709.1"
                     /translation="MPMLEVKNLYKNFDANEVLSGVSFNVEKGEVVAVIGPSGSGKST
                     MLRCINLLEKIEGGTILVGGDIMVSTEANGKVIYAPESQLREIRLRLGMVFQSFNLFP
                     HFSVIQNITEAPVCVAKMNRKTANSDAMELLAKVGLEDKAYAYPYQLSGGQCQRVAIA
                     RALAMNPEILLFDEPTSALDPELTAEVLKVMQDLAAEHMTMVVVTHEMSFARDVANRV
                     IFMDKGLIIEEGKPADIFTNPSNDRTRTFLRI"
     gene            23846..24772
                     /locus_tag="PK733_03850"
                     /pseudo
     CDS             23846..24772
                     /locus_tag="PK733_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624555.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            24820..25731
                     /locus_tag="PK733_03855"
     CDS             24820..25731
                     /locus_tag="PK733_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09710.1"
                     /translation="MKRKRKFNQIAPGWNIVISTLLILTSLLIVLPLILVVIISFSSA
                     ESIAFNGYTFFPSEFTLNAYKNLAKTGAQIKNSYIITVFYTFTGTVLSLFVMSMCSFV
                     LAQKKFPARKALTFYVFFTMLFSGGLVPSYIMNVRYLHLNDTIWIFLLPGIVNSFHII
                     ILRTFIQTTISDSLIEAATIDGANDFTIYYKIVLPLSKAGLATVGLFGLVGRWNNWFT
                     GMLYVENPKLIPLQTMLYKIQKDIEFIKSNAEISDSVMGLELLRSMPTESTRMAITIT
                     ATIPILFAYPFFQRYFIKGLTIGSVKG"
     gene            25804..27408
                     /locus_tag="PK733_03860"
     CDS             25804..27408
                     /locus_tag="PK733_03860"
                     /inference="COORDINATES: protein motif:HMM:NF023435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ09711.1"
                     /translation="MRKTFFSKVFLAVIILSLVFTGCNGSNSKKEPASTGADKITTTS
                     DAPPSNLKPVELVWYTAVDYIRADMDMVWNEINKYLKEKINVTLDRHFYPMADYREKI
                     STVVSSGQYMDILFTGASYSFAANAHQGAYYAIEDLIPKYLPETKKIVPQGGWDALTI
                     DNHIYGVPTYKDMADRFCFLYNKTMADKHNLKVLQNGKWATFKDMIPLLYEAKTARDA
                     DQPDLAKQPIIEMLNGIKRYYPFESLNGLAVANIPGVEAYEGKGSGETVFNLYETKEY
                     RELCKTIKKLVDDGIFPYDANNFDPDRALLNSGKLIGNFSGGYIEIKPDMYTGRETAL
                     STSSLVVMTTEYVQSGLQALSSKTKNPDRALMFLELLNTDKTLANMVRFGIENEHYVY
                     NSEGRLDITISPRNKDIASYTDYAYYFWYGWQFGNILAGDLPSVVSTNFGNLLKELND
                     SSIQDTNLGFAVNTKPIANEIAACSNVINEYDSNTNLRSGMVEDLDKTIDEFVAKLKA
                     NGSQKIVDEIQRQLTEWRKSVGKTTK"
     gene            27828..28709
                     /locus_tag="PK733_03865"
     CDS             27828..28709
                     /locus_tag="PK733_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09712.1"
                     /translation="MLSLDFDILWVSKCTYPAKHVIKKNYHDYYQIVFILNGEGEIIL
                     GNNAYNTSVNQVYIFKPNVEHSIKASRFKLLNIVEVKFYCNNTISESLVYQLPSIIKD
                     TGQSIRTAFVNIVEEVKMQDKYTEYIINSLFTQIIFFLVRISETEPSYSCRKNIDRVN
                     YKDDRSIRNEKEGDEPLTAIIDYIMQNYFMEIKLIDLAEIVYLSPIYFCSIFKERYGV
                     SPIQYLQSVRLENAKKLLIDTNNSITTISERVGFQSVNYFSRYFKTHEGITPNEFRRR
                     NQNFIIKDFRGDITDFS"
BASE COUNT         9618 a         4439 c         5817 g         9058 t
ORIGIN      
        1 tttagggggt acgagggtac cgttattgca gaaggccaat ttatttccgg ttatttatgt
       61 tggctctaac atatggaaga acttagggtg gagttctata atatatctat cgcaattatc
      121 atcaatagat cctcaattat atgaagcagc gacgattgat ggcgccggaa gattccgaaa
      181 aatgtggagt ataacaattc ccggaatact tcctacaatt attatcctct taattttacg
      241 tataggacgt atgctcagta taggatttga aaaaattatc cttttgtaca atccgttgac
      301 gtatgaaaca gcggatgtta tttcaacatt tgtctaccgc aaaggactta tggatttcgc
      361 ctggagttat tcaacagccg ttggacagtt taattcggtc gtgaatctgt tattgctggt
      421 attagcaaat aagattagta aaaaggttaa tgaaataagc ttatggtagg tgaaaggtat
      481 gaatagaaaa ctaaaaacgg attatatatt tgatactata aattatcttt ttatgtttat
      541 acttatattg gttacattat atccactatt gcatgttttc ctggggtcta tcagtgatcc
      601 atataggctg cagaagcaca gaggtatttt actgtatcca ctgggctttg atttggaatc
      661 ctatgcaaga gtatttgcta acaaatctat agtaacaggt ttcaggaata gtgtgtttat
      721 tactattaca ggtgtattac tcagtatgat aatgacaata cttgcagcat tttttctgtc
      781 acggcgtgat atcctgctta aaaagcacgt tatgtggttt attgttttaa ctatgtattt
      841 tagcggcggc ttgataccgt cctatatgaa tattaaggaa cttggtcttt ataattcaat
      901 atgggttttg ataatacccg gagcgctatc aacatggaat gtaatcctta tgcgcactta
      961 ctttttaggc ttgcccaacg aattggaaga agctgcaaca attgatgggg ccagcgaata
     1021 ttatactctt acacgtataa ttataccgct atcaaagcct attatcgccg tgcttgtttt
     1081 gtattatgga gttgataaat ggaatgaatg gtttgctgct tcaatttatt tacgcgatag
     1141 gaagatgtat ccgattcagt taatactgcg tgaaatactc atcaataatg acccggaaaa
     1201 aatgatggaa gatgttatgc tcactgataa atcagcttac cgcgagacaa ttaaatatgc
     1261 aacaacaatg gcagttacac ttcccatatt gtttgtatat cctttcctgc agaagtattt
     1321 tgtgaaaggt gttttattag gatctctcaa aggttaagga ttgaagccgg taatagttat
     1381 ttattgtatt ataagtattt tggtgacttt ggtattaact tttggtataa tataaacagg
     1441 ttgttttcac aggagggtca tatgaagaaa caggtcatac aaaatagagg agtcttattc
     1501 aaatggcttt tatcctatat ggtggtattg gcattaccac taactaccag ttacttttta
     1561 aataaagtat caactgatgc actaaataaa gaaattcatc tggtaaatgc caatgtatta
     1621 actcagatac gcagcagtat tgatgaaaaa ctaaaagaaa tggaaaagct tgcgttattt
     1681 cttggttcag attccaattt gcttggttta gttgaaaaga atgctgataa aaaaatgacc
     1741 tttcactatg acttacataa gcttgttagt gattttaaat cactattagc tgcgaatgat
     1801 tcaatatcag atttttatgt tgtttttcat aacgcagata tagtggttac accaggagca
     1861 gttcttaacc atgagcaagc ctatagtata ttgcataaga accaggaaat gagctatgtc
     1921 caatggaaac aacaggttat gaaaccttat atccggggat atgtaacatg cagtcggcga
     1981 ctaagtactt caagtgaagt taataccaga gtaatagcat atatgcacag tcttatatca
     2041 aatgatctgc aaaatgctgc agctacagtt gttgttatga cggatgagag gcagttacaa
     2101 ccctatcttt ctattcaagg ggaattacag gattcggaaa tttatctact aaatgcaaat
     2161 aatgaagtta tgttgtcttc aacaaaatca acagatcttc cagtgcagct aaattttgat
     2221 gatttaaatg aaggtgtgtg gaagaatata acaaccaaag gtatagaact ggcggtaatc
     2281 aataataaat cttctataac aaattataag tatgttggat ttatggaata tagtcagaac
     2341 tggaaaagac tgatggatat gcgtaaactg tcactgtgcc tgatattggc aggctttatt
     2401 ggaggattcc tggctgcctg ctggtttaca tggcgaaagt atgttagaat cagtgattta
     2461 cttcaattta tagtcaaaaa tctaaaaata aaaaaaccaa aacaggatga cgagtttgta
     2521 tttataagaa atgcaatgaa tttgactatt aatgagaagg aaaatttaaa gcaggaatat
     2581 gaccagcaaa agggtatatt gttccgcact tttttaaacc gtcttttacg tggagaaata
     2641 tctcctctta ctatgactga ggatactata aggttttatg ggtttcagga tgttactcct
     2701 aattggatgg tagtactatt ccactttgat atgaaaaaat cttcaggata tgaagagtac
     2761 agtttaatac ggaaaaaaga tatactttcg gaaataaaag aaatacttaa tcagaatata
     2821 ccacaggggt atggtattta tattactgaa atagatgata tgatagcagc tctggtaaat
     2881 actaatgaca gaaatccggg aaaactgcaa aatatatgca ggaaggcagg agtagtactt
     2941 aaagagatgg ctatttgcgg aattaccata tattctgctg taagcagcat tcatggtaaa
     3001 tcacttcagg gtattgctac ctgttatacc gaggcattgg aagtattgga atatagtata
     3061 atgtttggtg tcaatacggt aatgtcatat aaccagttag gaaaacatta tcaaacattt
     3121 gaatattcta tagatatgga gcaaaaactc attaacaata tacgctcggg aaatgctgat
     3181 agcacgaggt ctttgttata tgaggtgttt gaaaagaata tatcaaaaaa caaaatagga
     3241 cttgatatgc ttaaaattct tatgcttaat cttgtaagta cagcaataaa agctgcagat
     3301 agcaacactg taagcaaatt aatggaagat attcagccgg ttcgttgtat tgattcttgt
     3361 caaactatac ctgaagttaa acagcagatg acaagcattt tattatatgt ttgtgaaagt
     3421 tgcaaagaaa atgagaaaac ccggaatgag aaattatgta gcgatgttat ggattatata
     3481 aaagagaact atactaatcc tgagttaagt gttacatata ttagcgaaaa atttgagttt
     3541 tcaccaatat atttatcaaa tgttttcaat aaatgtgcag gagtaagtat tatggattat
     3601 gcccatcggt gccgtgtaga aatggcatgt gagtatctaa ataattctta caagtctgca
     3661 aaggaaatcg ctgcattggt aggatatata gatatcaata catttagcag aaactttaaa
     3721 aagtgtaaag gtataacacc gggtgaatat cgtaaaattg ggcctgaatt ggaaaatcag
     3781 ctttcatgac atagcttgat actgcaggct tgatactaca agcttgatac tgctagctta
     3841 atactactat attctgataa gcgtaaaaag tttcgaatat acaactaaaa ttaagtattg
     3901 attctactgc attaacttag atttactttt ttaatagtgt attgaaagga aacaatgatt
     3961 tgaaaagttt tacaatccat aaagattcga taatcgggag taatacagga ttctggaaag
     4021 cagcagggtc ggatttgctc ttttggagtg ctttaagacc agaaggacag gacttgctta
     4081 atagaatgat gaaaaccggt tcatgccggt attttagatg ccattacact ttttccaaaa
     4141 gaatagaata caatgctcag gttggtgggg aagtatattc agaagacgaa aaaggtaatc
     4201 cttgctatag ctttgagaag attaatagag tatttgagga atatttaaaa catggtataa
     4261 aacccattgt ggaatgtgat tatttaccgg atgaactgaa tattgaaaag gtgagtgggt
     4321 atctggatga aggactagcc aatagcacta aaggtccgaa taattgggaa aagtggtata
     4381 accttataaa gaattttgtt aaaaacctaa ttgacagatt cggagctgag gaggtgcgta
     4441 gttggtattt tgaagtatgg aatgagccag ataattttcc tatagaggaa ttaagcacct
     4501 tttttaaaat gtatgatgtc tttgttgatg cggttacttc tattgataac caactgaaag
     4561 tcggcggacc ggcttgcttt acaccggcat ttttaacgca ttttcttaat catgtagcca
     4621 atggaactaa ttatgtgaca ggtaaaacag gtactcgcat agattttata tcatatcata
     4681 tatatggaat gtccggtaaa tggataggcg aatatccatt agttcagcca acagtacaaa
     4741 gatttataca tgaaatactt tgggttcagc gtataataaa aagatttccc agacttgaga
     4801 atgtggaatt tcatctgaat gaatggggag tctgttctaa tttttatttg acctctgatg
     4861 agtatccggc tttagaattt agaaatagtg aattttccgc actctttctt gtaaagctgg
     4921 ttcacagtct ttatgctcta tgcgatatct ttaaatttcc catatcagta cttttgtact
     4981 gggggtttgc atatgaagcg gcaggtaaca ggctatttca tggaatgcgt gatttagtaa
     5041 cggtaggtaa catcccaaaa ccgatccaga ctgcatatga gatgttagca atgcttggcg
     5101 aacacaggtt gaaagttgcg ggaggaaagc ccggaggaaa tattgggctt ctagcgacag
     5161 taacaggtga ttttgagata caatttatta tatataactt ctgtgaacta catgaattta
     5221 acttaaaaga acacaatgac aggattgagg atagagatgg tgcagaagaa attgaaataa
     5281 ttattgaaaa ccttccaggt gggaaatata tggaagtaac atcctattat atggatagcg
     5341 aacaccataa tacatataga atatggcaaa acttaggagc acctcccttt gcttctaata
     5401 caaatataaa tattctaaaa gaaagtgggg agttaaaacc gaattacttt gaaagggtgc
     5461 ccattaattc cgggagtgca aaattaagat tcaggttaca aggaaatagc atgagactat
     5521 atttgggaaa ggctgtatat aaataaaagt ttggttttat ttgaatagca gtagaatatg
     5581 gcattcataa cttcatgtgg agggaaacat gatgcaaatt gtgcaagttg tagttgaata
     5641 aaaatagaaa tagacaggag aaacaggggg gattagtatg aacagcaatc aaattattga
     5701 aaaagtctgc aaagctatag atgcacttat tacctcaggc gagaagtata atggactctt
     5761 cccgtccatg attgacaggg attcaaatga aatgattatg gaatttactc cattgaatga
     5821 cgggcagcgt gagcatgacc gcacatttct tggaagcaac ctgatgcatg atcatgtagt
     5881 gttaaaaact atatatgcta ttggaaatgt tttaaacaaa aaaaattaca ttgaggctgc
     5941 agacaagtat ttgaagtact ttgtatcaca ttgcacaaat actgtaaccg gtctttttcc
     6001 atggggtgaa cacgcttatt ggcaccttct ggaagataag gcggcagcag ggcaggattt
     6061 gaaaccggat tctacagggg gaagcaagct tgtacatgat catttgaacc aggcgccttt
     6121 gtggttatgg gaaaagttgt atgaattcaa ccccaatgtg gttattagat ttgccaaagg
     6181 acttgatatg cactggaaaa gcaagcaccc atatgagtat aacaggcatg catacattaa
     6241 cattaaggag ccattcaaaa tgaagaaaac atattgggct gatttcccgc gacatggagg
     6301 cttctatatt tttgattggg cattcgctta tagaaaaacc agggataaag agttttatga
     6361 ccagataata accatgttgg attatatacc tgcaagtaaa gataaaaacg gtttactttc
     6421 atatgccacc aatagtattg gaagagatga attattgaag gctcctgcac agacactatc
     6481 acttgctgcg agtatgctgg aagcagcgga aatattatat gaaagtaacc ttgaattaag
     6541 catcaggctt cagaaggaag ctggtgaata tctcaatgga tttttttcgg taccgcatga
     6601 caatgatgaa gatgtctatg tattgtcatg tcgtcccgaa acctatgaaa ttacagggac
     6661 tatgcctata tggagcagta aatacggagt acctccggca gcttattccg gtcttatatg
     6721 caactgttgc tatcgtataa caaaggataa gaggctactt gactatgcga aatctgttgc
     6781 tatttcatat gttaaaaaag attttccgga cagcttgggg atacctgccc tggtttgcgg
     6841 gctggtattg ggtttgtttg cagatttata tgaaatttcc ggtgagcaaa attggttgag
     6901 tgaaggatta aagcttgctg atattgctat caaagtttat cttgacaagg atttgccaag
     6961 atgtgcaact ggaattgata tatatgaatc tcaaatgctt cccggatttt tgcttaatgg
     7021 acttgcaaga atagctttgc tttctgaaaa caggattaac tgcggtattg agcctgatta
     7081 ctcttacagg caatgaaatg caggccatgg aattgctttg cggtatatga tataatatcc
     7141 tcataaatga ggatattata tgcttgataa acgttgtata attaatatac atgccgtaaa
     7201 tataaaaaac atgccggttt catcgttgat ttcattgttc ctgaatttct ttcttgatgc
     7261 gtctttgggc tgggccttca attatatcgc catcataagt aaagcgtgaa ccatggcatg
     7321 ggcaatccca ggatttctct gcagaattcc attgcagttc acaacccatg tgagtgcatg
     7381 tagtgtcaac aagatgaagc cttccctgtt catctttata tgcacccatc ttttgaccgc
     7441 ccatttcaac aaccctgcct tctccaggat taagatcaac atcatcgcta tccggaactg
     7501 ctgcaagttt tcttactaca agatgttttg ctacatctgc attttcaacg ataaagttct
     7561 tggctgatgc accaggtgta aaacgtgaag gattatatag ttctttccag ggattttccc
     7621 tcccgattat taaatccctt aaaattattg aggatgcagt actgttggtc attccccatt
     7681 ttctgaaacc tgttgcaaca tagatattag gtgttttgga tgaaagatga cctgtgtaag
     7741 ggacctcatc tgctgatgta tagtcctgag tagaccatct gaatggaata tcttctactt
     7801 caaagatttt ctgtgcaaag ttttttaagt tctcataatg taccgatgta tctttgccct
     7861 ggcctgtttt atgattttct cctgctatga gtacaacccc tccatcatca gtattctgat
     7921 aacgtaaaga atggacaggc ttttctgccg aaatatacat accttcagga aattcgcctt
     7981 tgatttttac agcaactata taggatctat aaggaaaaag cctggaaaaa taataaccat
     8041 aaccatcata aaatggaaaa tgtgaagcca caacaacctt atcagcggtc actcttttac
     8101 cactgttggt tactacatgg cagtatttgt cttcatgaat atcaattgcc cttgtatgct
     8161 caaaaatata gctcccattg ccaggtattt catttgccaa tgcaataaga tacttaagag
     8221 gatgaaacct tgcctggtta tcaaaccgaa cagccgcttt aacagaaaac ggcaaaggag
     8281 tttctacaag gtagaaagcc tgaattccca ggcttgctgc ggtatttgtc tcatccagaa
     8341 tttgttgaat atacttgtca tcctgggtat atacatatgc agatgaacgg tagaagtcac
     8401 agtcaatttt ctttcgttct atcagggacg caattgtatt aattgcagtc tcgtttgcat
     8461 cagcatacat cttggccatt tcctgtccca tctggctttt aattttgtag taaattaagt
     8521 catgctgtga tgtaactttt gcggttgtat gtccggtagt accgtggagt atttggtcag
     8581 cttctataac tgcaactgtc aatccctctt ctttcaataa aaatgcagac gttattcctg
     8641 tcataccacc gcccacaatt actacatcta ctttaacatc ttcctccaga tgaggaaaat
     8701 ttgtttgtgg tatcgaggat atccaatagg attcaggtgg ttttgcgaat ccattacttg
     8761 tagtgttgtt catatcaggt attaatacca tcctttccat aaaaaaatat caataaatat
     8821 attttttgct catttaaacg aaaatatccg tatgaattta attccacaca tttttttatc
     8881 cagggttgga atgactaata gtagacataa tatatattta tatatattta aattacatgt
     8941 taaaaactac atgttaaaaa ctttggttag acatgaaaag cttaaatgct tgaagattac
     9001 ttgtcattga aataatgagt attgtaagag gaggcgtgtg taatatggag gaaaatatgg
     9061 aacacgtaga caaatccata gcattacctg caaatttgga tttcgaaata gagaaagcaa
     9121 tacagatgcg ggaaacattt aataagcttt ttaataacct tttgcagcag ggtgtagatt
     9181 ttgacaggat acccggaacc gataagccta cacttttaaa gccgggggcg gaccttctat
     9241 gccaggtatt tcattttgcc agtggtgaac ctaaaatgtt gtctataata gaagattttg
     9301 aaaagggaat attaagttac acagtatcga tacctgttat acaccgtgat acagggacgt
     9361 tggttgcagt tggcatagga agcgccaatt ccttcgaagt gaagtataaa taccgctatg
     9421 ttgatgctga tggaggtgaa aaggttcgtg tagaaaaccc agagcctgct gaccagcaaa
     9481 acacattggt gaaaatggct gccaagaggg catatattga tggagtcctg aaagccaccg
     9541 gtgcgtccag gatgtttacc caggatatag aggatatgcc atggctgcag ccggaaaaag
     9601 caagcagcaa gcagttaaat tacatcaggg ctttgtataa ggatgttcca gacgataaaa
     9661 ttcttgaaga tgcagccggt attattaata gaaatattga aaatgtggac gatatttcca
     9721 gggaagaagc atcaagaata atcgaagtaa agaaaagtaa aaattaccct cagtatcctt
     9781 tatacaccca ggaacaggcg gatacaaaat cagatttgaa gtatcaggct aaaaagaaaa
     9841 cagaagcata ctcatgtgca aactgtggtg taaggatcac acaaggagta gctgcatata
     9901 gcagaaacaa gtttggcaaa cttttatgtg ggaattgtca aaaatcaaca gatgttttta
     9961 atccggcaca accggataaa gataattata gtggtaattt tcaacggaat gaaactgagc
    10021 atgacagcac gttgcctcct tcctaataag tgctaagaag tggaaaagtg ggggacagtc
    10081 ctgcacaaag agaaaaaaat gcaggactgt ccccctgaaa ttcgtaatgt gcccctaaaa
    10141 ttctcaataa tttatcaaca tttcttattg acataagttt agaattgtgt tattaatatt
    10201 attgtttatt tgtttaacgt tactaaacaa aaagtttagt taattaaaac tttttttgac
    10261 aggggcttgt gggtgcatat gaaaataggt gagaaaatca agcgtctaag gctaaaaaat
    10321 gggttaaccc aggaagaact ggcaagcagg tgtgagctta ctaaaggctt tatttcacag
    10381 gtagaaagag aactgacttc tccgtctatt gccaccctgg ttgatatact tgaatctctg
    10441 ggcactaact tgaaagactt tttcaatgat attgtagaag aaaaggttgt tttcaaaaag
    10501 gacgatgttt ttgaaaagca ggacagtgag cttgagcata cgataaagtg gcttatttca
    10561 aatgcccaga aaaacagtat ggaacctatt ttaatgtatc tggatgccgg tggaaattca
    10621 ggtgaagatg atcctcatga tggtgaggaa tttgggtatg ttttgtcagg cagcatacac
    10681 ctttacctgg gagcaagaaa acatagagtt aagaaaggcg aaagttttta tttcaaacct
    10741 tctatagttc acagcatttc aaacgcagga aagactgaag ctatagtact ttgggtatca
    10801 tcgccgccaa gtttttaaat gtaaagggag ggaggccaaa tgccggaacc aattatcaga
    10861 cttataaata tatcaaaaga ctacaacgga gcagaagcac tgaaaaatat taacttatat
    10921 ataagaaaaa atgagtttct tacgctactt ggaccaagcg gttgtggtaa aactacaaca
    10981 ttacgtatta ttggagggtt tgagcagcct accgttggtg atgtaatgtt tgaaggtaaa
    11041 aacattacta atttgcctcc ttataagaga agagtaaaca cggtttttca gaaatatgct
    11101 ttgtttccgc atatggatgt agctgagaat atagcttttg gattaagaat aaaaaagctt
    11161 gatgaaaagg ttataaagaa aaaagtagct gaaatgcttg aacttgccaa tcttaaagga
    11221 tttgaaagaa ggcaggtgga ttctctgagc ggtggacagc agcaaagagt agctattgcc
    11281 agggcattgg taaatgagcc tgaagtgtta cttcttgacg aacctttagg agcattggat
    11341 ttaaaattaa gaaaagacat gcagattgag ctaaaaagaa tacaacaatt actgggtatt
    11401 acatttatct atgttaccca tgaccaggaa gaagctctta ctatgtcgga tacaattgtg
    11461 gtaatgaagg atgggacaat tcaacagatt ggaactccgg agatggtata taacgaaccc
    11521 gaaaacgctt ttgttgctga ttttataggt gagagcaata ttattacagg taaaatgata
    11581 aaagaccggc ttgcatattt tgcaggcaga gaatttgagt gtgttgatgc aggctttgaa
    11641 gaaaatgaga atgtagatgt agtagtaaga ccagaggata taaaacttgt tagggaagca
    11701 gaggggatgc tccacggaga ggtagaatcg gtaacattta aaggtgtgca ttacgaaatg
    11761 ataattaaga gccgtgatat aagatggatg gttcacagca ctcttatgga gcccgtaggg
    11821 agcagaatag gaatgcttat tctgcctaat gatattcata taatgaaaaa ggtgggtgaa
    11881 aaatgaaaag gacatggtat tcttatccat accttgtttg gatggtaatt tttattgtta
    11941 taccactttt acttttaata ttttttactt tcactataag caccgatgaa ggtataaaat
    12001 ttactatgga acacataaga aggctatggg atcctatata tttaatggta tttctccgct
    12061 ctgcaggtct tgctcttata agtacagtag tttgcctttt ggttggttat cctgttgctt
    12121 taatacttgc aagcagggat tacagaaaca agaaggctat ggccatgctt tttgttattc
    12181 ctatgtgggt aaacttcctt ttgaggactt atgcctggat gaccttattg gaaaaaaaca
    12241 gtttggtaaa tattattctg ggttacctgc atcttcctca gttaaagctt ctttatacaa
    12301 atcaggcagt tgtgcttggt atggtatata attttctacc ttttatggta cttccaattt
    12361 actctgtatt ggtaaaaatg gatagaaatg taatagaagc ggctgaagat ttgggtgcag
    12421 attacctgac tattttaaaa aagataatca tacctttgag tttaccgggt gtaatgtcag
    12481 gaatcacaat ggtatttatg ccggcaataa caactttcgt aatatcaaga ctcttgggtg
    12541 gaggacatta tacccttata ggcaacctta tagagcagca gtttatattt gcaggagatt
    12601 ggaattttgg atcagccctt tcctttgttt tgatgattat tatattaata accatgggaa
    12661 ttacccgtaa atatgacaag gaatatgaag gaggtggcgc aatatggtaa gccgtatagc
    12721 aaaaaaagca tacttgtatc tggtattcat atttttatat gctcccataa ttgtgctggt
    12781 aatatattct tttaatgaat ccaagtcaag aggtcgatgg ggaggattta catttaaatg
    12841 gtatggggag ctcttaaaag atagggaaat tgcagctgcc ctttataata ctttattagt
    12901 agctttactt tcgtctataa tagctacaat cataggtact gctgcagcta ttggaattca
    12961 gaatatgaaa aaaatcagga aagccataat aatgaatctt acttatttac cggttttgaa
    13021 tcctgatata gtaaccgggg tttcactcat gatactgttt atattcctga aagcttttac
    13081 acaattaggg ctgggctttt ttacaatgct gcttgctcat attaccttta atataccata
    13141 tgtaatactt tcagtgttac ccaagttgaa gcagcttaac aagcatatgt atgaagcagc
    13201 tcttgacctt ggagcaacac cggggtatgc attaagaaag gttattattc ctgaaataat
    13261 gccgggcata atcacaggcc ttctcctggc ttttactctt tccatagatg actttgttat
    13321 cagctttttt actaccggtt cgggggtatc taatctttca atatatattt actcaatggc
    13381 aagaaggggt ataaacccta aaataaatgc actttcaaca ttaatgtttt taacagtgct
    13441 gattttgctt ctcattgtta acaagagaat gtcaaaagat gctacaagtg gaaatgcgaa
    13501 ggaggttgta agatgaaaaa aattaaaaaa tctattgtta aatctaatga aaaatctatt
    13561 gtcaaatcta ttgcggcagt tcttattatt actgcatttg ccggattgtt tgccgggtgt
    13621 gggaaaagca acaaggcaaa actaaaggta tataactggg gagattatat tgatgaaagc
    13681 gtaattgagg aatttgaaaa aaaatatgag attgatgtag tctatgacac ttttgcaacc
    13741 aatgaagata tgtatgttaa gttaaaagga ggcggaagtg attatgatgt agcctttcca
    13801 tcagattata tgattgagag aatgataaaa gaaggcatgc tggagaagat agattttaac
    13861 aatataccta actacaagta tattgatgaa cgatttaaaa accttgaata tgatcccgat
    13921 aatgaatatt ctgtacctta tatgtgggga acggtgggta ttatttacaa taaaacaatt
    13981 gtaaaagaac ctgtggacag ctggaaaatc ctttgggaca agaagtactc caaacagata
    14041 ttcatgttgg acagcagtag ggattctata ggtataacac tgaaaatgct cgggtatcct
    14101 attaatacca gagacaaaag tgaactggaa gcagctaaaa atgctttaat tgagcaaaaa
    14161 ccgctggttt tatcctatat gggtgatgat ataaaagatg caatgatagc cggccaggcg
    14221 gcattagctg tggtatggtc cggggatgct gtttacatga aaagagaaaa caccgacctg
    14281 gaatatgtaa ttccaaaaga aggcagcaac ttgtggtttg atgccatggt cattttaaaa
    14341 ggtggaaaga acaagaagga agcagaaatg tttattaatt tcctctgcga atctgaaatt
    14401 gctttgaaga atactgatta tattggttat tccactcctc atactgaggc aaagaaaatg
    14461 cttgatcctg aagtactgaa cgacaggacg gcctatccta cagatgatga aatagagaat
    14521 tgcgaagttt ttcaagctct ttctgatgta ataaaggact atgatagaat atggacagag
    14581 gttaaagcaa aatagtccaa ttacttatac agataattat taatataaac gaaaagccgt
    14641 ttatcctgtc tttcctgaag acctgggtga acggctttat ctacagctag actacttttc
    14701 ttttaatgtt gcgaatttgt atctcatgct caccagttat ttctacgatt gacgtaatat
    14761 tatcagccat attctgaagt ttttgattca cttctgcgcg gaattcggtg agcattgctg
    14821 tttgttcgta aacagtatcc agtctacctt caacagtatc caatctacta tcaagagcat
    14881 ctaatctact atcgacagta cccagtctac cctcgacagc agccaatcta ctatcgacag
    14941 tatccagtct accctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctac
    15061 cctcgacagc agccaatcta ctatcgacag tatccagtct accctcgaca gtagccaatc
    15121 taccctctat ccgagtttgg ccttccttca gctcttgaac atcagatttc ataattccta
    15181 tttctgattg tatattgttt aaaatattta gaattttttc ttcgttattc atttgtactg
    15241 gctctccttt caaaattcgg ttattcgtat tattatagtc atgaagtgga atttctaata
    15301 aatataagtc ggattctaat tgttattacc aataataaca taaataacat ataatgtaaa
    15361 tggtttttta aaatctccgc tttcctgcag gaatttgcag aaatttatct actactggaa
    15421 aatcggctta aatcctacac ttctgcttat aagaaactgt tcttcttcag ctgatagtgg
    15481 tttaaagtct cgtgcaactt ccagcgccca tctgaaaaac tttatatacc ctggaggaat
    15541 tgctgctgtt acaggttttg aaagggtgta gcgtatagcc aactgtgcca gttctttatc
    15601 atctattggc tcataccagc atttaggata ttccctttct aagccttcct tccataaggt
    15661 ctttgccata gattttaatg ccagcattgt aatcttcttt tcctgagctt tttctattat
    15721 tctcttacca aagcccttat taaacatatt cacccaatta agagggaaaa gtaccgattc
    15781 aaattcaaat tgatttatta gttcttctgc ggcttcttcg gaatgggtag aaaaacctat
    15841 atgccggatt aatccttttt ccctggcttt tgttaatgtt tccagagctc cattaggtcc
    15901 taaaaccttt tttacatcat caatggttga tacactatgc agctggtata aatcaaaata
    15961 atctgttttc aatcgctcca gagattcgta aagggctttt tccgtttcta ccttgcttct
    16021 tttctcagtt ttacatgcaa ggaatatgtt atttcttttt ccaacaagag ccgggcccag
    16081 acgttcctcg gcatttccat agcttggagc tacatcaaaa taattcactc ctgaatcaat
    16141 tgcttctgct acaaagttgt ttgcatcttc ctgaaactca ttcataacaa taataccgcc
    16201 aaaacctaca atagaaagtg tttcacccaa tttaccatat agccttttat ccataaaaac
    16261 cctccaaact tatatttaac caaatgtata gaattgaaga atacatttta attttaccac
    16321 agaaagagta ataaagacaa actaaaatta aatgaaccct aaatgtacct caaacttaat
    16381 tttctaattg acacggtctg ctatagtagt aaaataggaa tgggctatgc atgctataac
    16441 aatacaagat atgagagtaa tacatataat aaccgtaaaa taaacccagt gggagtggag
    16501 ttttgaataa caattttaac ttagctgcag attcatctat tatttataaa gcatattatc
    16561 gttcaccgat tggtgtgatt tcgattcttg gaaataataa aggaattgtg gcagtagatt
    16621 ttattgatga agaagaaagt acctgcgatt taattataaa ggtatcaaat gaagagaaaa
    16681 taccacaatt accacgatgc attcaggaat gcatcaggca gttcgatgaa tattttgccg
    16741 gtagactccg caattttaca gtagatattg taattaacag gggaactgat ttccagaaaa
    16801 aagtttggaa tacattgagg gaaattccat atggagagac ttcaagctat gctttggtag
    16861 ctgaaaaaat aggaaaccgg aaagccgtaa gggcagtggg caatgcaaat aacaggaacc
    16921 ctataagcat aattataccg tgtcatcgtg tgataggaag cgatggcagc ctggtgggtt
    16981 acgggggcgg gttatggcgt aaaaagtggt tgttggagca tgaaagaaca aatcatgtat
    17041 tgaaacaaat tacatattga tatgatgcag tattttacca ttgtatcatt atgcattttt
    17101 tatgcataaa tctattgatt ttttcttaag tattatataa tctatatagt atcaataaat
    17161 attatatagc tgtgcaataa tttgcacagt agaatatttt agatgtattc ccgaaaggga
    17221 gaaaataaca tgaaatatgg gggtttaaaa atgaagaaaa ttttgaaaaa tattgctgtt
    17281 ttatttttaa taacaacaat agtattggca gttgccggtt gcagtaacaa ggggactctt
    17341 aaaatgggta ccaacgcaga atttccacct tttgagtttg ttgagggagg aaaatttgcc
    17401 ggaatcgatg tagaattagc agaagaaata gctaaagagt taggtatgaa acttaaaatt
    17461 gaaaacatgg catttgaatc acttattgat gcagttagca gcggtaaagt tgacttcgta
    17521 gctgcaggta tgactattag acctgataga gaagaaaatg ccgattttac tatgacatat
    17581 tacaacgcta ttcaaacaat tattataaaa gaggataata gcaatattga aacgaaggat
    17641 gatctggaag gaaaacgtat tggggtacaa accgggacca caggccagta tttagctgag
    17701 gaaattaagg acgcagaggt tggtaaatat aataatggtc tggaagcagt tatggatctt
    17761 aagaatggga aaattgatgc agttataatt gataattttc ctgccaaaac ctatgcagat
    17821 aagaatcccg agctaaagct tattgaaggc caatttgagg aagagcagta tgccatggct
    17881 gtaaaaaagg gaaatacaga gcttcttgaa aaaataaata atgcactgca aaaacttata
    17941 gataacggca cttaccagaa aatagttgat aaatatagta agtagttaaa tgcacccgta
    18001 gttaagtagg ttcatattgt tgaatagcaa tcctgaagta atatagaatt tttgggttga
    18061 aataattttg ttttgggagg tttctaatgg tttttattga aaacctgtat agagttttaa
    18121 gtgacaaaat atattatgct tttataaaag aagatcggtt tatgtacctg gtaaatggtt
    18181 ggtcaatatc ggttcaattg actttatttg cagcaatttt aggtatattc atcggtatgt
    18241 ttatagctat tgcaaaaatt acagggaaac ctccttttgc ccagattgct gattggtatg
    18301 tatctattat aaggggaact cccgcagtag ttcagctaac tataatttac tttataatac
    18361 ttgcagatgt aaatctgcca aaaacagtta tagcatccat tgcttttgga ataaatagcg
    18421 gtgcctatgt atctgaaata ataaggtcag gaatccaggc tgtggataaa gggcaaactg
    18481 aagcgggaag atcacttgga ttatcataca gccagacaat gcggcatgtt ataattcccc
    18541 aggcggtgaa gaacatactt cccgctctgg gtaacgaatt tattatattg cttaaagaaa
    18601 catccatatc agggtatatt gctcttgatg accttactcg tggtggaact cttataagaa
    18661 gtgttaccta tgattctatg acgccccttt taactgttgc atatatttat tttcttatga
    18721 cagcaatgtt atcagctctt ttggcaaaat ttgaaaggag gcttcgctca agtgattaag
    18781 gtaataaatc tgcataaata ttttggggac ttagaagtac ttaaaggagt aaataatgaa
    18841 ataaaaaaag gtgaagtcgt atgtgtaata ggaccttcgg ggtccgggaa aagcacattt
    18901 ttaagatgtc ttaatcttct tgaggttccc acacgcggag aagtaattat tggtgatatt
    18961 gctcttaccg ggaatatgaa ggatatagat aaactgagaa gacatgttgg aatggttttt
    19021 cagcagttta acctgtttcc tcatatgact gttattgaga atatcatgct tgccccggta
    19081 aaagtgctga agaaaagtaa agaagaggct gcagcagaag ctttggagtt attaggacgt
    19141 gtaggcctgg cagataaagc aaatgaatat ccgtcaaggc tttcgggtgg tcagaaacag
    19201 cgcgttgcta ttgcaagagc aatggcaatg aatcctgaag taatgttgtt tgatgaacct
    19261 acaagtgctc ttgaccctga aatggtagga gaggttttgg aagtaataaa agctcttgca
    19321 aacgaaggta tgacaatggt aattgttact catgaaatgg gatttgcaag ggaagtttcg
    19381 gatagggttc tatttatgga tgaaggaatt atttacgaag aaggaacacc agctcagata
    19441 ttcagcaacc cgcagcggtc caggacaagg gaatttttaa gtaaagtgct ttaaaagtac
    19501 tttagaggtt agaacttttg gaagcaaggt aagtagtaat ctgatattag aagctgattt
    19561 aagataaagt agcacactaa gaaaaatatt caatatgaag taatgccttg aacatttgag
    19621 tacagagttt agttaaataa gggagaacgg tatgtatata gcaaaagtaa tttatatcgc
    19681 aatagcggta cttacaatat tgcctcttat tgcgtttaat atcaaaagat tcactgttaa
    19741 aagggaaggg gaaaatccac ggcctaatat tgttattatc attttgacac ttggagggag
    19801 tattctcctt ggggttttct tattttctct ttataaattt acccttgatt atcaaccaca
    19861 actagtattg gagcgattta taagatctta ctcatccacg gcggcaggta agatagatta
    19921 tgagaaattt ctaaaagaga cagagagcat atgcacacct gaatttttat cccttaaaga
    19981 cagatgttat agggaaattg aagctgaccg ccaggcaggg ctgggaacag taagctttca
    20041 actgggtgag accatattac ccaagtatta ttttgataag gaatttttcc ctaatgtgga
    20101 aggaataaaa caaaatgata aacagccaat ctacatgctg tctatgattg aatatggtga
    20161 aaccaggaaa ttttatataa ctcttttacg tattcaagga aacaagtggt atgtggatag
    20221 ttttaatgaa gcttcaaagg agcttgctga atatgcatac cggtataatt tcatgaaatc
    20281 tgaacacgca aataaatggt ttaaagtgaa aaagtaagca gtattcagta aagaaaagaa
    20341 tgaagcaaat attgagcttt attttaagtg aaattctagc ttaaatgctt tactaagacg
    20401 cagttataaa gtaaaatagt aaagtgtatt tatactatac ttaacagtgt attttcatca
    20461 caatagttat gatacattaa ctacatataa tagtagatat aaaagcaaat ataaacaata
    20521 gaaaagggga tgtttttgtg agaaaatatt tattaaagaa aataatcgta gcggtgcttt
    20581 cgctggtgct ttcagcttca atgttattga caggttgtgg agattctggt tctaaagctg
    20641 ataattcatg ggataaagtt aaagaaaaag gtgaatttgt tttaggattg gatgaaagtt
    20701 ttccacctat gggattcaaa gatgataagg gggaaattgt tggatttgat attgatacag
    20761 ctagagaagt atgttcacgc cttggtgttg aattgaaaat tcagcctatt agctgggacg
    20821 caaaagaaca ggaacttaat acaggtaata ttgattgtat atggaatggg tttacaataa
    20881 ctgatgaacg aaaagaaaat gttctattta ccaagccata tatgaacaat cgtcaagttc
    20941 tcgttgttaa agaagattca ccgtacaatg atattaagga tcttgaaggt aaaaaacttg
    21001 gcctgcagtc tgagtccagt gccgtcgacg ctctggatgc caatgaagat tttaaggcaa
    21061 aactcaaaga agtgatatta tttgacgaga acatgatggc tattatggac cttgagaaag
    21121 gcggagttga tgctttacta attgatgaaa tagtaataag gtactatatg cagacaaaag
    21181 gtaataattt taagattttc gacaaagagc tttctcatga agaatatgga gtaggtttcc
    21241 gcaagggaga taagaaactt atgaagaagg ttgaggaaac tcttgaagcc atgtcgaaag
    21301 atggcaagtt aacagaaata tcaacaaagt ggtttgatag agatatagta actataaaaa
    21361 aataaatata atatataact cagatttcgg tgacaaatta tgcaacgtgt ttctatgctt
    21421 cattcgcctt ttgcaccgaa atctgagtta atgcagtaga atcactttat atatattttt
    21481 ttacataaag gccggcacag tataaaaccg gcttttttaa gtataggaat ccatgaaatt
    21541 gttaaaaggt tcctatactg aggtttggaa aggggattcc ccttcctgct ttaaggtaag
    21601 attgcaattg tagagcgaac aagaaaattc acatcattgc ctgagtcatg agcaaagcgg
    21661 gttttctcgt tagagtgctt atctgcgcct gaatttggca tgattgtatg ttatgccaga
    21721 ttaatcgata ttgaggggaa gaacaaaatg cttgaaaata taaattggaa tgaaatagcg
    21781 cttgtgttgg aagactttat aaaaaatttt gtaagattat gcaaattgct gggtgcaggg
    21841 ggactggttt cactaaaagt atttttcctt acattgatat tttcactgcc ttttggatta
    21901 ttgatagctt ttggcaggat gtcggagaaa aagtggataa atggtccagt cagacttttt
    21961 atacttatta tgagaggtac tccattgctt ttgcagctta tatttgtgta ttttacccct
    22021 tcttatttac ctgaaccaat aagaattaac ctggatcgtt ttacagcagc tatcatagct
    22081 ttttccttca attattcagc ctactttgca gaaatataca ggggaggcat agaatcaatt
    22141 tcacaaggcc agtatgaggc cgcagcagtg ttgggcttta ctaaaattca aacatttatc
    22201 aggattatac ttcctcaggt aataaaaaga atattgccgt ccataagcaa tgaagtaatt
    22261 acgctggtaa aggacacagc tctggtaatg tctcttggaa tttcggaatt atatagggca
    22321 gccagaaatg aagccgcgcg ggtattttcc acaagtccga tttttatagc gggtttgttc
    22381 taccttctta tgaattatgt tgttacaaaa gtttttgaca aaattgaaaa aaaactgagc
    22441 tattacaggt agatggcttg gttctggctg agttatgggg gaagtgagta gtggataatg
    22501 gatagtggat aattaatgct actattccaa ttcggaaagc gatttccatt cgtaacataa
    22561 caaattagca ctaaattaat acttaataag gatgatagga attatgaata taccaatgct
    22621 tgaagtgaaa aatttatata aaaattttga tgccaatgag gtgctttcag gagtttcttt
    22681 taatgtagaa aaaggtgaag ttgttgctgt tataggtccc tcaggatccg gtaaaagtac
    22741 tatgttaaga tgcatcaatc ttctcgaaaa aatcgaaggt ggaacgattt tggtcggggg
    22801 tgatattatg gtaagtactg aggcaaatgg caaagtaatc tatgcccccg aaagtcaatt
    22861 gagggaaatc aggcttcgat taggtatggt atttcaaagt tttaatctgt ttccacattt
    22921 ttctgttatt cagaatataa ctgaagcacc tgtttgtgtt gcgaagatga atcgaaaaac
    22981 agcaaatagt gatgctatgg agctgctggc aaaagtagga cttgaagata aggcctatgc
    23041 ttacccttat cagctttcag gcggacaatg ccagagagtt gccatagcaa gagcattggc
    23101 tatgaatcct gaaatacttc tttttgatga gcccacatcg gcattagacc ctgaacttac
    23161 cgcagaagtt ttaaaagtta tgcaggatct ggcagcagag catatgacaa tggtagttgt
    23221 tacacatgaa atgagctttg caagagatgt agccaacagg gttattttta tggataaggg
    23281 acttattata gaggaaggca aacctgcaga tatatttaca aacccttcta atgacagaac
    23341 aaggacattt ttgagaatat agcggctgta ttaggttttg ccatatgatg ccgcctgatt
    23401 tggctctcga ttaaataaac gccgctacaa tccattgcta aagtgaatat tatatgagga
    23461 aatcgtttat tgccatgttt tttcaagtac agaaaatacg ccctcagtaa tcagtaatca
    23521 gtggttttaa tgctttgggt gtatgcttca aacttccttt ctcttatact gaatgctgct
    23581 gaatacttac taaatactac taaatactgc attagacgaa aaaagtttgc ttggtatgca
    23641 gactttttga gaatagttat atacaatctt aataatgtgc aaaaacaaat taatattttt
    23701 caaaaaaaac aattggttag atgttataat aaattataca ttattgataa aaaaattgtg
    23761 caaatttaca aaacctttgt gttattatac tgaatttatt acatcaaata gcttagttac
    23821 ttaattactt atggggaggt atatgatgaa attttttcaa catatatgga atagccatat
    23881 caaacattat tatattctgc atttaatgat cttacctaca atcattgcat tgcttatatt
    23941 cagttatata ccaatgctgg gacttgttat ggccttccag agcatgaata taggtaaagg
    24001 aatttttgga agcgagttta taggatttaa aaactttgag tttttattta tgactactga
    24061 tgcttggata attacacgca acacggtttg ttataatgtg gtatttatac tccttaatat
    24121 gttcttggct gtaacccttg cattactatt aagtgagatt cacagtaaga aacttgccaa
    24181 aacacttcaa actatattta taatgccata ttttctttca atgaccgttg tatcaattat
    24241 tgtttttgca tttttaagcc ctaatagcgg tttccttaat aatatattgg aacttcttgg
    24301 ttttcagcgc caaagttggt atatgaaacc taaattctgg ccattaattt tagtaatagt
    24361 aaatgcatgg aaaaatgtag gatattcagc agtagtatat ctagctgcaa tatctggaat
    24421 atccgaagaa tattatgagg ctgctataat tgacggagca aataaattga agcaggcatg
    24481 gtatataact attccgcatt tgaaagctat gatgactata atgctgattc tttcggttgg
    24541 tggtatattc cgtggtgatt tcgggctttt ctataccgtt ccctaggata gtggtgcttt
    24601 atatcctgtt actgatgtaa ttgatactta tatataccgt tctttaacga cattaaacaa
    24661 tgtaggcatg gctactgcag caggtcttta ccaatcagtt gtgggctttt tattagtttt
    24721 gacaaccaat aaagttgtca acaggatatc acctgataat gccatgtttt aaacaactaa
    24781 aataaatgtc agatgcagta aataaagatg gaggtttgta tgaaaagaaa aagaaagttt
    24841 aatcaaatag ctcccggctg gaatattgta atatctacat tattaatact aacatcactt
    24901 ttgattgtgc ttccacttat tttagttgtt attatttcct tttcaagtgc agaaagcatt
    24961 gcgtttaatg gatatacatt ttttccatcg gaatttactt taaatgcata taaaaatttg
    25021 gcaaaaacag gggctcaaat taaaaattct tatattataa ctgtttttta tacctttaca
    25081 ggtacagttt tgagtttatt tgtaatgtct atgtgttcct ttgtattagc acagaaaaaa
    25141 tttcctgcaa gaaaagcatt aaccttttac gtatttttta ctatgctttt ctccggtggt
    25201 cttgttcctt catatataat gaatgtgaga tatttgcatc ttaatgacac tatatggata
    25261 tttttattac caggtatagt aaattcattt catattataa tacttcgtac tttcattcaa
    25321 actactatct cggatagttt gattgaagcc gctactattg acggtgcaaa tgattttaca
    25381 atttactata agatagtttt accgctatcc aaagcaggcc tcgctactgt tgggttattc
    25441 ggcttagttg gcaggtggaa taattggttc accggtatgc tttatgttga aaatcccaag
    25501 cttatacctt tacaaaccat gctgtataag attcagaagg atatcgaatt cattaagtca
    25561 aatgcagaga tttccgattc agtaatggga ctagaattgc taagatcgat gcctacagag
    25621 agcacacgaa tggcaattac tattactgca acaataccta tattatttgc atatccattt
    25681 tttcaaagat attttattaa gggtttaaca attggtagtg ttaaaggtta gtttattatt
    25741 ccactaacac atagtggaaa taaaacataa taaacaaatt ctattcataa ggaggatttt
    25801 tgtatgagaa aaactttctt tagtaaggtg tttctggcag taatcatttt atctctggta
    25861 tttacaggat gcaatggttc taattctaaa aaggaacctg catctaccgg agcagataaa
    25921 ataaccacta catcagatgc accgccatca aaccttaaac ctgtagaact tgtatggtac
    25981 actgcagttg actatataag ggctgatatg gatatggtct ggaatgaaat taataaatac
    26041 ttaaaagaaa aaattaatgt aacactggac cgtcatttct atcctatggc agattataga
    26101 gaaaaaatct ctacagttgt aagttcaggg cagtatatgg atattctttt tacaggtgca
    26161 tcttatagtt ttgcagcaaa tgcccatcag ggtgcatatt atgcaattga agacctgatt
    26221 cccaaatatt taccggaaac aaaaaaaata gtgccccaag gtggatggga cgctttgacc
    26281 attgataacc atatttatgg tgtacctacc tataaagata tggctgatcg tttctgtttc
    26341 ttatacaaca agactatggc agataaacac aatcttaagg ttctgcaaaa tggaaagtgg
    26401 gcaacgttta aagatatgat tcctctgcta tacgaggcaa agactgcaag agatgccgat
    26461 cagccagatc ttgcgaaaca gccaattatt gaaatgctta atggcataaa gaggtattat
    26521 ccttttgaat ctctcaatgg tcttgcagta gcaaatatcc ccggggtaga agcatacgaa
    26581 gggaaaggca gtggagagac agtatttaat ctttatgaaa caaaagagta tagagaacta
    26641 tgcaagacta ttaagaaact tgttgatgac ggcatatttc cgtatgatgc taataacttt
    26701 gacccggata gagcattgtt aaattctggc aaacttatag gtaacttttc cggtggatat
    26761 attgaaataa agccggatat gtataccggc cgtgaaactg cactctcgac ttccagttta
    26821 gttgtaatga ctacagagta tgttcagtca ggtttgcagg cactttccag caaaactaaa
    26881 aacccggacc gtgcattgat gttcttagaa cttcttaaca ctgacaaaac acttgctaat
    26941 atggtacgtt tcggaattga aaatgagcac tatgtttaca atagtgaagg aaggcttgat
    27001 attaccataa gcccgcgtaa taaagatatt gctagctata cggattatgc gtactacttt
    27061 tggtatggtt ggcagtttgg taatatcctc gcaggcgatt tgccatctgt ggtttcaacg
    27121 aatttcggga acttgcttaa agagttgaat gatagctcta ttcaggacac aaatctcggg
    27181 tttgcagtta ataccaaacc aatagcgaat gaaattgctg cttgttccaa tgttataaat
    27241 gagtatgact ccaacaccaa tttaagaagc ggcatggttg aagatttaga taaaacaatt
    27301 gatgaattcg ttgccaaact taaggcaaat ggatcacaaa aaattgttga tgaaattcaa
    27361 aggcagctta ctgaatggag gaaatctgta ggtaaaacta ctaaataatt gcttgcaatc
    27421 agttatttga acaaaatgaa aagagataat tagtgtaaaa gaaggaagca aaaaggttgc
    27481 agtccttttt gcttcctaaa aaagccttga ttaaagccag gtaaagcttt gattgaagtc
    27541 aaattaagct gacaaaagct aacaagaata aataaggggg ggttaagaat aacaaaagtg
    27601 aataggagtg aatatgaggt aataagaatc aatataattt gatgacaata ttgcaccatt
    27661 aggaacatat caacaacttg atttatggcg agaacaagca tataattaaa tatattgaaa
    27721 atcatattta ttagtatatt aagggttcat tattgtgtga atgctgatta tatggttaaa
    27781 aattaattat taatggacag ttgtatagtt gatggaaggg agggcttatg ttatcacttg
    27841 attttgatat attgtgggta tcgaagtgta cttatccggc aaaacatgta attaagaaaa
    27901 attatcatga ttattaccaa attgttttta tactaaatgg agaaggagaa ataatattag
    27961 gtaataatgc ctacaatacc agtgttaatc aggtgtatat atttaagccc aatgttgaac
    28021 attcaattaa ggcttctagg ttcaaattgc taaatatagt tgaggttaag ttttattgca
    28081 ataatactat cagtgagagc ttagtgtatc aattaccatc aattattaaa gatacaggac
    28141 aatctataag gacagctttt gtaaacattg tcgaggaagt taaaatgcaa gacaaatata
    28201 ctgaatacat aataaactcg ttatttactc aaataatatt ttttttggta agaatatcgg
    28261 aaacagagcc aagctattcc tgcagaaaga atatagatag agttaattac aaagatgata
    28321 gaagcataag aaatgaaaaa gaaggcgatg agccattgac tgctattatt gattatatta
    28381 tgcagaatta ctttatggaa ataaagctga ttgatcttgc agaaatagtt tatttaagtc
    28441 cgatttattt ttgctcaatt tttaaagaaa gatatggagt ttcaccaata caatatctcc
    28501 agagtgttcg acttgaaaat gcaaagaagc tgcttataga taccaataat tctattacaa
    28561 caatatcaga aagggtagga tttcagtctg tcaattactt ctcaagatat ttcaaaactc
    28621 atgaaggtat aacccctaac gaattccgta gaaggaacca gaattttatt attaaggatt
    28681 ttcgcgggga tattactgat ttttcctaga atatgtttaa ttatgcttgc ttatattaaa
    28741 tttccatata ggatgtaaga ttcttgctga aatacatcta tgaatgttta tgcatacata
    28801 ttaatatgta tgtgtgctac tacaagaata ctgtaagaaa aatccatgtt gatagtatta
    28861 ctagtatttt taaagaattc acaattaact aggaggactt ttcatgaaag acaaagcaat
    28921 aataggaatt ataggttgtg gaatgatagc tcgggatgta catcttaaaa atgcattttc
    28981 aaaccccagg atacatgtga aatggtgctg tgatttatcc gatgagaggt ta
//
LOCUS       DAOSDD010000024        28167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_786_length_28167_cov_5.035287, whole genome shotgun sequence.
ACCESSION   DAOSDD010000024 DAOSDD010000000
VERSION     DAOSDD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 28167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28167
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_28167_cov_5.035287"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..12179
                     /locus_tag="PK733_03870"
     CDS             39..12179
                     /locus_tag="PK733_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ09713.1"
                     /translation="MKVTAEGYEHTYPVTEFFSDLIFPNTDGTITVGIPAMSYAAEGR
                     RAVEVFKGEVVIARTGFNVGDSKIMYANSHEYWNEVAIGGEYLENYLNSNPKAEVWLN
                     GEKIASSISYRFMGELLFRFPTSFEPGANYRIKVFVNDDEKTEAEGFPFSVAPRLDLD
                     NPIVSIAEGNCTLIIKESSYAEYGWQSDDAITVHVHGPEFYKRFETTGGNLTVNNDTV
                     VFTLKDGFGNNLSLITGEYNVDVDKDGAKIGYGEFIVVQNDGDAIKGSVNNPDGSAAD
                     GGYADIHKVDQGWGTRIILGHDGKFYIRKERLKGTGDYEFIAIPSSSSQYASSSRVVT
                     LDENGDIKDKDGSILNTVDITLNKAQVSGTVYVYSGGDKANGGRVDIHEVYDNNNWKW
                     ITGEDIASDGTYKIGGLKPNTKYQIVAIPPREIDYLHSDTVEIITDDSASFPTPVDLS
                     FTKPQIRGIARDDGNELLNSGQYYINARSLYYHGSYKTKTGDNGIFMIGGLAPGEYMI
                     SIEPESNSIYVRSEEIKVVIDQNGIASPSSIELKLAQPKLTGSVIDKDGNTLSSDGYV
                     EVYKGTWKNMQFISSVRVDINGTFRIGGIPDGDYYIRAIVNYGSEKFSSNSSSVFTPI
                     KLGTTETVNLQLTDRSVPRIRWIMDTGVGSGFVNLHMYNLAGKTKDAFAAEILDAEGS
                     SLTPSIQIPAENINITWVDEFSDEAGLEIYSESIKNLKAGKYKLKLSYNGVEISSDSP
                     LQNEFIVLYYMDVSPKGALPSTAPGMLLKLFVNDGKGGKVSGIWKEGDTLSAKLITSN
                     GVEYHNTIPCTINTDDTISINLPTDITLPDGWNYIEIYNKINTESILLGKADFVVGTP
                     AIRRVQDANEGDNNVNVEGDYLAVYEKQGVSGEILDLTGKVLWTSAYVNFAWERLEFR
                     FDDLQLPAGNFKFRIKDGTTVIGENSFNVVPQLICTPRVVPDQSAATLTVKNSSSLSE
                     FKWSSNDSITDFRVYIGNSGPWFDIDSGNGLSFADDGSGNLIINISAENMQKLSFGEG
                     KAQIDVYKNHGEADFRKIGFARFVLGYDIISGTVKNLENEPMPGVRVNIENISEYDRR
                     GFSVFTDGEGKFNISKSDMPWPGDEIPTGDYSFVVMAPKGSSYGSAITKVNIDPAVTN
                     NVILALPPAQITGTVKAGSENATGSRVEVLKPIDDRKWEYLYYIDVNSDGTFSFGGLD
                     AGTYFIVAHAPEGLLFLHSPAEEVVIGDTVQDITVNLSPVQVVGAAWDAKGNLLEGYE
                     YYLRVRSLEPEKGENDNGKWRTEYGKYYLGGLKPGSYAINMQPNRDSLYTQSLEEIFT
                     VNPDGTVSREVIDLKFTNPKVIGAVRYIKDQEMLPLTEGYVDIFKGTWQNMHYITTVE
                     LDENGKFRIGGIPGGKYYIRAAIWDESPLRQDYSQSFMKEIIISDDIPETPEEIEEPL
                     MLMDKTPRIRVVYDAMTGDDNLRLRMVNASKLVNTSGVVSGLTAEIMDQYGNPLANPI
                     EISNPGNFSIDNRTWDFNKDEADILIRLSGNLNLKPGNYNLKLIYNEGTGYKQVVNET
                     PFINGFNGFRILYWLHVNPYGVLPGTASLGKIIEISADGDSSQYPWNTGDSDKLIIKL
                     TTEDETEYTLPCSIDANNIIKAELTSPPIPSEGLAKGRYRITVYKDEVLLGVSELHAS
                     EPYINGIFDSCEENDFITLYGINLITYEENDIVGKVYRISDATPVVTSSKVDFNWGHL
                     NISLNNTRLIPGDYRLELYVDGAEIALPEDGIFTVANLLRATPSYLLTGNTSGKTVTL
                     DAAEGITPWEIGDKLFISISCDNYGNEEYGPISVTAESVSSITFTMPDIVNEGHCSIF
                     VYNENDPDHIKYIGYAHFEILEQTQLTGTIKDFAENPVPYSSVLVFRTDEKTGEHWVM
                     RFDADGEGNYAVYGIEPGEYTVFAIPPAGKVLFESDSLELVINDDMTGTIPSPCDFIL
                     PEARVISGTISIPESVTENRYIHVHAFTDKGTPDEADDIGTGSEVLIPAGETSVLYAI
                     NVPVGYSYRVQYFADPGYGYENGGFYSENGTVFDYYQATLLDLTTSGKSGIDLYVSKA
                     LAPSIVKAEAIEDGTAIKLYFSKPLDMMTLPSAPAGFTIKVSSDVYEISSVTANEKEN
                     ELTLELDNYKIFKDYTNITLTYGKEAGVKSYDGMLLESITTDEPVSISNLSNRVLSIV
                     TGFANGALLNKDARISVSTSEEAKIILKENESILVSGETSVTGTISSEGSHAITISVD
                     GRKNDITITFTIDKTPPDIAILNCDNTVVINNHDGVTPTIEFSSDCVAASKVVTLNGQ
                     AYDGSTIKRAGEYILKASARDEAGNVRTVSKEFKIIWDTGAPVIVVSGVENGKIYQDT
                     SIGISLARDAGIPQEYSNEANYYYKGSLKLPDGKVQTFDNNSIPSVTDKGAYRLEILA
                     VNPSYTDITSKKIIEFTIDKDIPKTEIGNLIHNHIYNTEVTPLISFTDDVASQQTLIA
                     NATVTLTKGDLPVKNYHIGDTITADGVYTLTAETKDAFGHESEVKSVTFTIDRTKPVI
                     TTFGVLNGYTYKDQDVTVTIKTNEGTLTVICNGNTIMPDGNGQLPTFKGEEDKLTVYK
                     LYAEAKDEAGNISGQELSFTIDRLAVNIKVNGISDGMLINYSPDITFTTFEGMNEKTG
                     TAAEIDGIAFAGGSYTKEGSHTMVLSYTSGGNTYTKSINFIIDKTVPAISYNNILKNN
                     DAATSVIYTKTGDSIKVRANISDTNGISEAYFSIGTAAAAIPMKYVEGSGYYEGEFFV
                     GSGNYTEMDIIITAKDKAGNSASPKHSNVKVIIDNTKPVVSIKTDPAIADGRNGIFKS
                     ENMKITLTAGTTETIYYNFNNNPGSAAGTIQLSPAQGTNILVYKAQDPAENMSDEKIY
                     IFEYDSIRPSDVILSDLTPETSLVNTETVGITGSVDNEGGKAGSKVLLKKANTGEVVA
                     SANIKADNTFVMDGIRLVEGLNSFTLTAMDLAGNESSTPTAISRTLDSTAPIIKVDKV
                     DDIHYTISANEDVTLIVVKFNGIVIDKAKIIVDPANARKYTVETPDPVEGTNTLNASA
                     ADTAGNIGHGSYTSTYIPANTSQTDVQLNDNSTMDIPGTAFTTSVQMLVKTVDVDDNI
                     SYKPLGAAMNFEFVDEESRPVAPEAPLVIRNYIGAGLKGVALMHVNADGTVDRTITAT
                     VTTSNVFDMYDNDTIDSLGTFDDNDINKGTVIYIKDTGYVVFKTKTFSGYAVVQDVTA
                     PEIVLSTSDFEINAADKSEGKMRIAGTLKKGGTGADSAEDDTTAYIMEVTIDSVVMDI
                     GGLTEEQKGYNFNIPLDLADGTHEVEIRASDASGNASTLTRTYQVDVTPPVLTAEAAT
                     DKTNRDSVDIYISTDETSEICINGEYAGHFYGNNIIPFTLKDGSENEISVTAKDAFGN
                     SSEAETIKVTRDSTAPSITISGVVNNAIYSTDREIVVSVTDTHDCEAVITMDGEAYIP
                     GIYTVEGKHTLRVDAADSFGNTSSSVITFTIDKSVPFIKISGAENGGVYNSNKVIEIS
                     VENADEITVEISTDGQAAQSIPMELAGGSIPLGAYGEKHDYKITVTAKRTTAERTLIS
                     VDIITLTIDRKEPPAVTPDDDTGDTGDTGGGTIPVTPVTPVTPKPDIPENPETDTPET
                     PEQVVPAVSEPVNGIITVEPVLTGNTANSAVTMEVIDTAFKQLKANAVGVKTIGIEVK
                     ELSDAKTYTQEIPASIMQAGSAKRNIAISMPYATVTIPGNMFTAADIGNKKTIEISVS
                     TVDIDELAGETGKNIDKDIINAIGNKPVISLSASIDGKNVAWNNPDAPVEISIPYTPS
                     SEELINNEHIVVWYIDGNGKVVAVPSGRYDVQTGRVTFTTTHFSKYAVSYVKKTFSDI
                     SDYSWAKKQIEVLASKGVINGKPGGVFDPGSNITRADFILLLVKALGFTAKADTNFAD
                     VKASDYYYEAVGIAKKLGITQGTGNNLFKPNEFITRQDMMVLVNRALLLQDKLSAPGN
                     VSDLSKFGDVGKIAAYAKESIANLVNEGIILGSNSNINPLGNALRAEIAVVIYRIYYK
                     "
     gene            12670..13602
                     /locus_tag="PK733_03875"
     CDS             12670..13602
                     /locus_tag="PK733_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09714.1"
                     /translation="MYKKKILIIDDTELIVKIIKDILETADYNVISASSGEEGLIKVE
                     QEKPDLVILDVVLPGMDGFQVCRMLRNDDRNNLMPIIILTSQCDEDDKLTGLELGADD
                     YIIKPFNPRELLSRVRNTLVRIDRNRYVNPLTGLHGNIEIQTEMNQKIASNEIFSLIY
                     ADIDNFKAYNDVYGFANGDIAIKLTADILNYNMRLFGTPGNFLGHIGGDDFIIITMPQ
                     CVDDICKGIIHDFDIKILNLYNEEDRKNGYIITTNRRGEPREFPIMTISMAVVSNEYR
                     DFKTYYHIAEVAAEIKKVVKSMNGSCYSKDRRRC"
     regulatory      13815..14028
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            14030..15238
                     /locus_tag="PK733_03880"
     CDS             14030..15238
                     /locus_tag="PK733_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOJ09715.1"
                     /translation="MHKVYRLEKFINAKIPLSVFAMVLLFALFSPIIYAESAKEGIVN
                     TECLNVRQSPCTSAAIAAQLLKDTKIEIDEFQEGWYKITYGNIKGWVHADYVTTEQTM
                     TVTENAGSNGSSINGASNGEPQKTGVITANVLNVRESAGTSSKVAAQLEKGMDVNVIE
                     TNNSNNSETWHKVSYGKITGWISGEYISFKYEPIDAGVVDVDVANVRTAPDISSKIAT
                     QMTKGQKLDIYAWSGEWYKIKLQDENFAWIFNELVSTRNSFASRESTGARGQSSVVDR
                     SLDKRQQIVSLAKKYLGVKYVWGGTSPKGFDCSGLVQYVYKQIGITLNRVAADQATQG
                     TKVSRAELKPGDLVFFNTSSGSSIDHVGIYIGGGNFLHAANGRGKVLIDPLDHSYYKN
                     RMITARRIIK"
     gene            15694..18354
                     /locus_tag="PK733_03885"
     CDS             15694..18354
                     /locus_tag="PK733_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOJ09716.1"
                     /translation="MKRPLVLFAIALAIGILFAKITQSYKFIVISALILLMVIIIIEV
                     KVVKNRMIILPLTGILLFYSIGGFEFLYNDRIISEKYQEYAGEQVTIKGVIDSEPEFQ
                     GKKITCVVYCKEIINNDKNKTNRNNADKFITGKISLTILTSNLQTAPAASAVQTAPAT
                     PVASVAQTTEAMLIDYGKTIEITGTLNIPAGKRNPGGFNYQSYLAGSGISATMYVKEN
                     SIRVGEESHANIFLKIGHLIRKRFINVINNSMPPKQAGLMNGMLTGYRGGISEDTKAV
                     FSDSGLMHIIAVSGMHVGFIMLPFIFLFKKMGVKKIVSNPLIILILILYVSITGLQPS
                     VLRAVIMASTVLLSQIIWKEPDTLASISFAAILLLIWNPHILFNIGFQMSFIATLSIV
                     LLRKNVSEIIKPAAKSAVKYITRSTKALSAHFVAHSDNHSDNHSVTRSIADSMPVYSQ
                     YSTNKSKPASKIKDRTEKLAKKVTDILGTTLSAQAGILPLSILYFNKVSIISVVSNLM
                     VAPVTGTVIVLGLFMAIIGQISLFAAKIAGYANNILLSFIIFISEFTAQIPFAILKTG
                     ISSIMLILVYYILVWFFLWLKPRHKIKVKPMSYAWAAICVILVISTINLIPGQLEVVF
                     LDVGAGDSIFIRTHGGRTVLIDGGGEKGKLDNEPDTGEKIVVPFLLGYGVKKLDLVVA
                     SHGHGDHIQGLMPVLKEFNVGKVILPDYIEGNEFDKILTICGERKINVTYCSQGDTIK
                     LDKKTYFEVLNPPRNIVPEIKMSLNNSSLVLKLYYKNVTMLFTGDIEKEIEEILADNL
                     LESGVHVFKVPHHGSETSSIGKFLELVKPQVAVISTGRNNYGHPAPSVLKRLEETGTG
                     IYRTDLNGAVIIRTNGRLIKVKTMLNGEQG"
     gene            18390..19409
                     /gene="holA"
                     /locus_tag="PK733_03890"
     CDS             18390..19409
                     /gene="holA"
                     /locus_tag="PK733_03890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ09717.1"
                     /translation="MSIDILKTQLKNNNIKSLYLFYGPEEFLKKFYIKSIEDQIVDKN
                     MAALNKVTLADKVDVQKIIDNCDTLPVFSERKLVIVKNSGLFKGGSGGSEGKKGGRNG
                     DALLDYIKNIPSYTCLIFYEDEIDGRLKVSKEIKSQGLVVEFPYQKPVDLVKWVYKVS
                     KTYKKEIDAEAAALLVEGCESGMTGILNEIEKLVAYTGDRNNITLEDVKKVSSATVKS
                     IIFDLTNAIVEKDMAASLKLLDDMIVLKEPAPKIIFMIARQFRQMLQVKILLESGNSP
                     KEISSRLKLQPFIVEKLRKISANFNIKRLKENMEEIFECDLAIKTGKMKDRTAVELLI
                     AKLTI"
     gene            complement(19500..19766)
                     /gene="rpsT"
                     /locus_tag="PK733_03895"
     CDS             complement(19500..19766)
                     /gene="rpsT"
                     /locus_tag="PK733_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ09718.1"
                     /translation="MANNKQAIKRIKIADKKALANKMRKSALRSTVKKSRTAIDNKDP
                     HAAEMLKEAVKLLDKAVVKNVIHKNNASRQKSKLTKAYNTAISK"
     gene            19927..20940
                     /gene="gpr"
                     /locus_tag="PK733_03900"
     CDS             19927..20940
                     /gene="gpr"
                     /locus_tag="PK733_03900"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOJ09719.1"
                     /translation="MIDKPERRSVVTDLAIEAHELVKEQTVRGAQQKEDNIPGVDVTN
                     AGTEDIKISRVKISSVQGQNALGKPMGTYITLDIPGIKENDIELYENACKALASELAQ
                     LLRLNEKSTTLVVGLGNWNVTSDSLGPKVVSKMMITRHLLQYVPEQVDQGVRPVCGIA
                     PGVLGITGIETGEIVRGIVDRLRPDCIIAIDALASRKLDRVNTTIQIADTGISPGSGV
                     GNKRMDISRETLGVPVIAIGVPTVVDAATIANDTIDKVIDSFIQKADEGTEFYNKLKN
                     MDRDEKYQMIQEILDPYTGNLIVTPKEIDEVVERIAKVIANGLNIALHQGITLDDVDR
                     YLQ"
     regulatory      21176..21262
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            21357..22961
                     /gene="serA"
                     /locus_tag="PK733_03905"
     CDS             21357..22961
                     /gene="serA"
                     /locus_tag="PK733_03905"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ09720.1"
                     /translation="MKVIVTDKMADDGIRYLRENGFEVDTPFGISPEELLEVIENYDA
                     IIVRSATKVTEKVIERAKNLKVVGRAGNGIDNIDVNACTKRGILVVNTPEGNIMAAAE
                     LTIGMIFALFRNIPQAYMAAKNKDFRRNKFVGNELDGKVAGVIGLGRIGTIVATKLKA
                     LNMKVIGYDPFVTDERCQKMGIKKCETMEDLLKEADLVTVHIPKTDKSQGLISEEELK
                     LCKKGVRIINVARGGIVNEKALYNALKEGHVAGAAIDVFEKEPNFNKKPEEQDYSNPL
                     LELDNVIYTPHLGASSEEANHNVSSQVAELIAGALRGEMVAAVNMPPMKVDNLNGLKP
                     YVEIAEILGKIYYQAEKPTVNKVEVIYSGDLAEKDTRLISLSALKGLLEHAVKENINY
                     VNAGYIAKTMGIEMVESKSSYLDKYTNLITLKFTTKDRELSVSGTVFTKDEIRIVDFF
                     GYKLDFEPTPYVIAIQNIDKPGIIGQIGTILGANDINIAAMQWSRNRKGEKAVAFVSV
                     DCEVCESILNQLRQINGVLKATMLKF"
     gene            23118..23486
                     /locus_tag="PK733_03910"
     CDS             23118..23486
                     /locus_tag="PK733_03910"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOJ09721.1"
                     /translation="MIGFLVFIIGLVFGSFMNVCIYRIPAHESVVTGPSHCPACGKQI
                     KWYDLVPVLSFLILRGRCRFCKTSISPRYPAVELLNGTIWMLLYCFFGLTPIFIILCI
                     VFSCVMVYGFIKYDGSKSAR"
     gene            23715..24134
                     /locus_tag="PK733_03915"
     CDS             23715..24134
                     /locus_tag="PK733_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ09722.1"
                     /translation="MIKKMLKSMGKNKKGFTLVELMVVVVIIGILTAIAVPVYNNVTK
                     SATDNANAANIRILEGAISMYLTNNPSDNVTFEDIIMDNTGTLSVPSGKTLNGGKKLV
                     PEYVTSMPKQPSSTLGYVKTNGAGKRVELEVSAAPEG"
     gene            24297..24596
                     /locus_tag="PK733_03920"
     CDS             24297..24596
                     /locus_tag="PK733_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09723.1"
                     /translation="MVVFIILSVLVALALRIYSTIETDLVKKILENNKLLLLNALQIY
                     LIEVGIPEGGKAPPGGWKQAIMPYLQEWPSHPLGILGDYVISGTYPDYKVEFVPE"
     gene            24999..25199
                     /locus_tag="PK733_03925"
     CDS             24999..25199
                     /locus_tag="PK733_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09724.1"
                     /translation="MQYADDVVVVNKPKPEKAGNRLEDINLGDMGHGFHDHSYSKGGY
                     GCEGRKEFKVNSTTETRDSEGA"
     gene            25212..25739
                     /locus_tag="PK733_03930"
     CDS             25212..25739
                     /locus_tag="PK733_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09725.1"
                     /translation="MLEETKERHKRTCCELRWHEHEVLGGRKIDCLELETSTETKEEH
                     VGRTSYREGIPQDRRWNMKKWYSLIDKIYQPENLTKAFKRVKRNQGAPGIDGETVEDF
                     NVKLEENIEFLHGKLKTNTYEPSPVKRVEIEKPDGGKRLLGIPTVSANCTKVQYPFGK
                     CHDYTPISSIMWPGI"
     gene            25759..25980
                     /locus_tag="PK733_03935"
     CDS             25759..25980
                     /locus_tag="PK733_03935"
                     /inference="COORDINATES: protein motif:HMM:NF036442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09726.1"
                     /translation="MNGTRLYSLRTDSGVMCVPESWTDRKVQPENVQNSRKIPFDALT
                     LRELAQLIQAIEGLPITEKKSVDKVNKER"
     gene            25990..26340
                     /locus_tag="PK733_03940"
     CDS             25990..26340
                     /locus_tag="PK733_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09727.1"
                     /translation="MNNNDKITLRKVPTRELKERKAQILSSLPPMDDIIRGSLITRSI
                     KCGKPNCRCATGDGHKSFYLSSYYHGNTYMDYVPKSWEAWISKGIKNYGVIQDLLSEL
                     TEINLELFRRRGKK"
     gene            26499..>28167
                     /locus_tag="PK733_03945"
     CDS             26499..>28167
                     /locus_tag="PK733_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ09728.1"
                     /translation="METSSKVRTEHLNRMAIVYIRQSTLTQVRFNRESTERQYALQEK
                     ALSLGWSQEQIQVIDEDLGISGSGRSQRQGFQQLVAKVSLGEVGGIFGLEVSRLARSS
                     ADLSRLLELCGLFGTIVVDEDGIYDLSDFNDRLILGFKGTMSEAELHFLRTRMLGGKK
                     NKAKKGELRFPLPVGYVYDADGQTVMDPDEEVQIAVHNVFKAFKSSGSAYGVVQYFVQ
                     NNLQFPKRAYGGAWAGKLVWGRLTHSRVLGILYNPAYAGAYVYGRYRDQKQVNPQGLF
                     IHHTVRLPKEKWEVFIPDHHTCYITWEMYEENHKQLQNNRTNIEKSGPAREGIALLQG
                     LILCGKCGRRMTVRYTGNGGIAPVYECKGRWEHGVKATCTTVSAPIIDKAVSNRLMQV
                     MQPAELELAIEVMDKLLEEAYDADKGWKLSLERAKYEVDRAERQYQLVEPENRLVVRS
                     LESRWNEKLAELTRIEEEYAKHRSRQSWRPTEQDKNMILSLAKELPRIWNAQTTTAKE
                     KKRILRVLIEDITVFAEPRQPNVRLGLRWRNHCSEEIYTTKPLPKGTA"
BASE COUNT         9668 a         4616 c         6102 g         7781 t
ORIGIN      
        1 gcaaacagcc tcctgcatgg ggtactgatc aagtaaaggt gaaagttaca gcagaagggt
       61 acgaacatac ttatcctgta actgagtttt tctcagacct tattttccca aatacagatg
      121 gtactattac agttggtatt ccagccatgt cttatgcagc agaaggaaga cgtgctgttg
      181 aagttttcaa gggtgaggtt gtgattgcta gaacaggctt caatgttggt gatagtaaga
      241 ttatgtatgc caactcccat gagtattgga atgaagttgc gataggtggc gaatatcttg
      301 aaaactattt aaatagcaac ccgaaagctg aagtttggtt aaatggagag aaaattgctt
      361 ccagcatttc ttaccgattt atgggcgaat tactattcag atttccaaca tcctttgaac
      421 cgggggcaaa ctacagaata aaagtgtttg ttaatgatga tgagaaaacc gaagccgagg
      481 gatttccttt ttctgtagct ccgcgtcttg acctcgataa tccgattgtc agtatagcag
      541 agggtaactg cacactaata ataaaggaaa gcagttatgc agaatatggc tggcaatcgg
      601 atgatgcaat aacagtccat gtacatggac ctgaattcta taagcgtttt gaaaccactg
      661 gtgggaatct tacagttaac aatgataccg tagtctttac attaaaagat ggattcggaa
      721 ataatttatc tttaattaca ggtgaatata atgtagatgt agataaagat ggtgccaaaa
      781 tcggttacgg ggagtttata gttgtacaga atgacggcga tgctataaaa ggaagtgtta
      841 ataaccctga cggttcagca gccgatggcg gatatgcaga tatacataag gtagatcaag
      901 gttggggtac gcgtataata ttgggtcatg atggtaaatt ttatataaga aaagagcgcc
      961 ttaaaggaac cggagattat gaatttatcg ccataccttc atcatcttca cagtacgcaa
     1021 gcagcagtag agtagtgaca cttgatgaaa atggagacat aaaagataaa gatggaagca
     1081 tactaaatac agttgatata actcttaata aggcacaggt ttcaggcaca gtttatgttt
     1141 attccggcgg tgataaagcc aatggaggaa gagttgatat acatgaggtt tatgataata
     1201 ataattggaa gtggattacg ggagaagata ttgcttctga tggtacatat aaaatcggtg
     1261 gtttgaaacc aaatacaaaa tatcagattg ttgcaattcc tccgagggaa attgattatt
     1321 tgcattcgga tacagtagaa ataattactg atgattctgc atcttttcca actcctgttg
     1381 atctttcatt cacaaaacct caaattcggg gaattgcaag ggatgatggc aatgagcttt
     1441 tgaatagtgg tcaatattat attaatgcta gatctcttta ttaccatggt tcttataaga
     1501 caaaaacagg agataacggc attttcatga ttggtggtct tgcccccggt gagtatatga
     1561 tctctatcga accagagagt aattccatat atgttagatc tgaagaaatt aaagtagtaa
     1621 ttgatcagaa tggaattgca tcaccttcgt caattgaatt aaaacttgcc cagccgaaat
     1681 tgacaggaag tgtaatagat aaagatggta atactctatc atctgatggc tatgttgaag
     1741 tgtataaagg cacatggaaa aacatgcagt ttattagctc tgttagagtt gatataaatg
     1801 gcacattcag aattggaggc atacctgacg gagattacta tataagggca atagttaatt
     1861 atggttctga aaaattcagc agcaactctt cgtcggtatt tacaccaata aaactaggaa
     1921 ctacagaaac tgttaatctc cagctcaccg acagatcagt acctcgaatc cggtggatta
     1981 tggatacagg tgtagggtcc ggatttgtga atttgcatat gtacaacctt gcaggcaaaa
     2041 ctaaagatgc ttttgcagca gagattcttg atgcagaagg ttcttcatta accccttcaa
     2101 tacaaatacc tgcagagaat attaacatta catgggttga tgaattctcc gatgaagctg
     2161 gtcttgaaat atattctgaa agtataaaaa atctaaaagc cggaaaatac aaactaaagt
     2221 tatcatataa tggagtagag atatcctccg atagtccttt gcaaaatgag ttcattgtat
     2281 tatattatat ggatgtttct ccaaaaggag cacttccatc aacagcaccc ggaatgctgc
     2341 tcaagctttt tgtaaatgac ggaaagggcg gaaaagtttc gggaatatgg aaggaaggag
     2401 atacattatc ggcaaaactt ataacaagta acggagtaga atatcataat actattccat
     2461 gtacaataaa taccgatgat acaatttcaa taaacctacc gacagatata acactgccag
     2521 atggctggaa ttatattgaa atatacaata aaataaatac tgaaagcata ctgctcggta
     2581 aagccgactt cgttgtagga actcctgcca tacggcgagt acaggacgca aatgagggcg
     2641 acaataatgt aaatgttgaa ggtgattacc tggcagttta tgaaaaacaa ggtgtatcag
     2701 gagaaatact tgacttaacc ggaaaggttc tttggaccag tgcctatgta aactttgcat
     2761 gggaaaggct tgaattcaga tttgatgatc ttcagctgcc tgccggaaat ttcaaattta
     2821 gaattaaaga tggtacaact gttattgggg aaaattcatt taatgtagta cctcaattga
     2881 tttgtactcc acgtgttgtt ccggaccaat ctgcagccac ccttactgtg aaaaacagtt
     2941 cttcgttgag tgagttcaag tggagcagta atgacagtat tacggatttt agggtttaca
     3001 tcggcaattc aggaccatgg tttgatattg attcaggcaa tggactttca tttgctgatg
     3061 acggtagtgg taacctaata ataaatattt cagcggaaaa tatgcagaag ctttcatttg
     3121 gagaaggaaa ggcccagatt gatgtataca aaaatcatgg ggaggcggac ttcagaaaaa
     3181 tcgggtttgc aaggtttgtc ctagggtatg atataatttc cggaacagtc aaaaacctgg
     3241 aaaatgaacc tatgcccgga gtaagggtta atattgaaaa tatcagtgaa tatgaccgga
     3301 gaggcttttc tgtatttacc gacggcgaag gaaagttcaa tatatccaaa tcggatatgc
     3361 catggcctgg agatgaaatt ccgacaggag attattcttt tgttgtaatg gcaccaaaag
     3421 gctcatctta cggttctgca ataacaaaag ttaatattga tcctgctgtc acaaataatg
     3481 taatattggc tctgccaccg gcacagatta caggaactgt aaaagccggt tcggaaaatg
     3541 ctacaggttc cagggtagag gtgttgaaac ctattgatga taggaaatgg gaatacttat
     3601 attacataga tgtaaacagc gatggcactt ttagtttcgg cggacttgat gcaggtacct
     3661 attttatagt ggcacatgca ccggaggggc tgctcttttt gcattcacct gctgaagaag
     3721 tggttattgg tgatactgta caagatatta cagttaacct gagtcctgtt caggttgttg
     3781 gagcagcttg ggatgccaaa ggtaatttgc ttgaaggtta tgaatattac ttaagggtaa
     3841 gatcccttga accggaaaaa ggtgaaaatg ataatggcaa atggaggacc gagtatggga
     3901 agtattacct gggcggactg aaacctggtt cctatgcaat aaatatgcaa ccgaataggg
     3961 attcattata tacacaatct cttgaggaaa tatttacggt taaccctgac gggacagtgt
     4021 cacgtgaagt tattgatcta aaattcacaa atcctaaagt aataggggct gttagatata
     4081 taaaagatca ggaaatgctg cctttaactg aaggttatgt agatatattt aaaggtacct
     4141 ggcaaaatat gcattatatc acaacagttg agctggatga gaacggcaaa ttcagaattg
     4201 gaggaatccc cggcggcaag tattatatac gagctgctat atgggacgaa tctccgttac
     4261 gtcaggatta ttcacaatca tttatgaagg aaattataat aagtgatgat attcctgaaa
     4321 caccggaaga aatagaagaa ccattgatgt taatggataa aaccccgcgc ataagggtgg
     4381 tctatgatgc aatgacaggt gatgacaacc ttagattgcg catggttaat gcgagtaagc
     4441 tcgttaatac aagtggtgtg gttagcggtc tgacggcaga aattatggat cagtatggaa
     4501 atcctcttgc taatcctatc gaaatatcaa atccaggcaa cttcagtata gataatcgta
     4561 cttgggattt taacaaagat gaagccgata ttttgatcag actaagtgga aatcttaatc
     4621 taaaacccgg caattacaat cttaagctta tatataatga aggaacaggt tataagcagg
     4681 ttgtaaatga aacacctttt ataaacggct ttaatggttt cagaatttta tattggctcc
     4741 atgtaaaccc atatggagtt ttaccgggga cagcatcatt aggcaagatt attgaaataa
     4801 gcgcagatgg tgacagcagc caatatccat ggaatacagg cgatagtgat aaacttataa
     4861 ttaagctgac tacagaagat gaaacagagt atacattacc ctgttccatt gatgcaaaca
     4921 atatcattaa agcagaatta acttcacctc caataccttc ggaaggtctt gcaaaaggca
     4981 gatatagaat tactgtttat aaggatgagg tgttgctggg ggtttcagag ctgcacgcaa
     5041 gtgaacctta tattaatggc atatttgatt catgtgaaga aaatgacttc ataacacttt
     5101 acggcattaa cttgattacg tatgaagaga atgacatagt agggaaggta taccggatat
     5161 ccgacgcaac accagttgtc accagttcaa aagtagattt caactggggc catcttaata
     5221 tatccttaaa taatacaagg cttattcccg gggattacag gcttgagctt tatgttgacg
     5281 gagccgagat tgcgcttccg gaagatggta tattcacagt ggcaaacctg ttgcgtgcaa
     5341 caccaagcta tttacttaca ggaaatacct ccggcaaaac agttacattg gatgctgcag
     5401 agggtataac tccatgggaa atcggtgata agcttttcat aagtatttca tgcgataact
     5461 atggaaatga agagtatggg cctatttccg ttactgccga atcagtatcg tctataacat
     5521 tcacaatgcc cgatatagta aatgaaggtc attgttctat atttgtatat aatgaaaatg
     5581 accctgatca tataaaatat attggatatg cacactttga gatactggag caaacacagc
     5641 ttaccggtac aataaaagac tttgcagaaa atccggtacc ttattcttca gtcttggtat
     5701 ttagaactga tgaaaaaacc ggcgaacatt gggtaatgag atttgatgcc gatggcgaag
     5761 gcaactatgc agtatatggg attgaacctg gtgaatatac agtttttgcc atacccccag
     5821 cagggaaagt cttatttgaa tctgacagct tggaactcgt aattaacgat gatatgacag
     5881 gtactattcc cagtccatgt gattttatcc ttcctgaagc cagagtaata tcaggtacaa
     5941 taagcattcc tgagagtgtt actgaaaata gatacattca tgtgcatgca tttactgata
     6001 aaggtacccc cgatgaagct gatgatattg gtacaggctc tgaagtacta atccctgcag
     6061 gtgaaacaag cgtactttac gcaatcaacg tacctgttgg gtatagctat agagtacaat
     6121 acttcgctga tccgggctat ggatatgaaa atggcggttt ttactctgaa aacggaacag
     6181 tatttgatta ttatcaggca acgcttttgg atttgacaac atccgggaaa tcaggcattg
     6241 acctgtatgt aagcaaagct ttagcacctt ccattgtaaa ggcggaagcc attgaggatg
     6301 gaacagctat aaaactttac ttcagcaagc cccttgatat gatgacatta ccttcagctc
     6361 cggcaggctt tactattaaa gtcagcagtg atgtatatga aatcagtagt gtaacagcaa
     6421 atgaaaaaga aaatgaactt acactggagc ttgataatta caagatattc aaggattata
     6481 caaatataac tctgacatat ggtaaggaag ccggtgtaaa atcttatgac ggaatgctgc
     6541 ttgaaagtat aaccactgat gaaccggttt caatttcaaa cttaagtaat agagtattaa
     6601 gtatagtaac aggctttgct aacggggctt tattaaataa ggacgcaagg ataagtgttt
     6661 cgacttcaga ggaagctaaa ataatactga aagaaaacga atcaatactt gtatcaggtg
     6721 agacatcagt aacaggaacg atctcttctg agggttctca tgcaataaca atttctgttg
     6781 atggcagaaa gaacgatata actataacct tcactattga taaaactcct cctgatatag
     6841 caatattaaa ctgcgataat acagtagtta taaataatca tgacggagta actcctacta
     6901 tagaattcag cagtgattgt gttgctgcct caaaggttgt aactctcaac gggcaggcat
     6961 atgacggcag cacaatcaaa agggcagggg aatatatact caaagcatcc gcaagggatg
     7021 aagcaggaaa tgtaagaaca gtaagtaaag aatttaaaat tatctgggac acaggtgctc
     7081 ctgtcattgt tgtaagtggt gttgaaaacg ggaaaatata ccaagacacg tcaattggaa
     7141 tatcattggc tcgtgatgct ggtataccac aggaatacag caatgaagca aactattact
     7201 acaaaggcag cctgaagtta ccggatggca aggttcaaac ctttgataat aattccattc
     7261 cttctgttac agacaaaggt gcatacaggc tggaaatcct tgcagtaaat cccagttaca
     7321 cggatattac cagcaaaaag attatagaat ttacaattga taaagacatt ccgaaaacgg
     7381 aaataggcaa tttaattcat aaccatattt acaacactga agttacacct ctaatcagct
     7441 ttacagatga tgtagcttca caacagaccc ttattgctaa tgcaactgtt actctgacca
     7501 aaggtgattt accggttaaa aattatcaca ttggcgatac aattacggca gatggtgtct
     7561 acacacttac tgctgaaacc aaagatgctt ttggtcatga atcggaagtt aaatccgtta
     7621 cctttacaat tgacagaaca aagccggtaa taaccacttt cggtgtcttg aacggatata
     7681 cttacaagga tcaagatgta actgttacaa tcaagacaaa tgaaggtaca cttacggtga
     7741 tttgtaatgg gaatacaatt atgccggatg gaaacgggca attgccaaca tttaaaggtg
     7801 aagaggataa attaacagta tataagcttt atgctgaggc taaagatgag gcaggaaata
     7861 tttctggaca agaattatcc ttcaccatag acaggcttgc tgttaacatt aaagtaaacg
     7921 gtatctccga tggaatgctg ataaattata gtccggatat tactttcaca acctttgaag
     7981 gtatgaacga aaagaccgga acagcagctg aaattgatgg tattgcattt gcaggcggaa
     8041 gctatactaa agaaggaagc catacaatgg tattaagtta tacaagcggt ggcaatacct
     8101 atactaagag cataaacttc ataatagata aaactgtacc ggcgataagc tataacaata
     8161 ttctaaaaaa taatgatgca gcaacatccg tcatttatac caaaacagga gattccatca
     8221 aggtaagagc taatatcagc gatacaaatg gtataagtga agcatatttc agtataggaa
     8281 cagctgcagc agcaattcct atgaaatatg ttgaaggttc aggctattat gaaggtgaat
     8341 tcttcgttgg tagtggtaat tatacggaaa tggatataat tattactgca aaagacaagg
     8401 ccggtaattc cgcttcgcca aaacattcaa atgttaaagt tataattgat aatacaaagc
     8461 cagttgtgag tattaaaaca gatccagcaa ttgctgacgg caggaacgga atattcaagt
     8521 cagaaaacat gaaaattacg ttaactgcag gaacaactga aacaatatac tataatttca
     8581 ataacaatcc gggcagtgca gcaggcacca tacaactatc acctgcacaa ggcaccaata
     8641 tattggtata caaagcacag gacccggcag aaaatatgtc tgatgagaag atatacatat
     8701 ttgaatatga tagtatcaga ccatcggatg ttatcctgtc agatttaact ccggaaacta
     8761 gcctggtaaa tacggaaact gttgggataa ccggttctgt tgacaatgaa ggtggcaaag
     8821 caggttcaaa ggttctgctt aaaaaggcaa atacaggaga ggtagtggct tctgcaaaca
     8881 tcaaagctga taataccttt gttatggatg gcattaggct tgttgaagga ttaaacagct
     8941 ttacactcac agcaatggac cttgcgggaa atgaaagcag cacgcccact gccatttcaa
     9001 ggacattgga tagtactgcc ccaatcataa aggttgataa ggtggatgat atacattaca
     9061 ctatttcagc taatgaggat gttacattaa tagtagtaaa attcaatgga attgttattg
     9121 acaaggctaa aatcatagta gacccggcaa atgcaaggaa atatactgtt gaaacacctg
     9181 atccggtaga gggtaccaat actctgaatg caagtgctgc cgatactgcc ggaaacattg
     9241 gacatggttc atatacaagt acctatatac ctgcaaacac atcacagact gatgtgcagc
     9301 tgaatgataa ttcaacaatg gatataccgg gtacggcttt tacaacttct gtacagatgc
     9361 ttgtaaaaac agttgatgtt gatgacaata taagctacaa acccttggga gcagcaatga
     9421 acttcgagtt tgtagatgaa gagagcagac ctgttgcacc tgaagcacct ctggtaataa
     9481 gaaattatat aggtgccgga cttaaaggcg ttgcactgat gcatgttaat gcagacggaa
     9541 cggttgatag aactattaca gcaacggtta ccacaagcaa tgtgtttgat atgtatgata
     9601 atgatacaat agacagtcta ggtacattcg atgataatga tataaacaag ggaactgtta
     9661 tttatatcaa agatacagga tatgtagtgt ttaaaacaaa aactttctcc ggctatgcag
     9721 tagtgcagga cgttaccgcc cctgaaattg tcctgtcaac ttctgatttt gaaattaacg
     9781 cagcagataa atcagaaggc aaaatgagaa ttgccggaac cctgaaaaaa ggaggcacag
     9841 gagctgattc tgcagaggat gataccacag catatatcat ggaggttact atcgatagtg
     9901 ttgtaatgga tataggagga cttactgaag aacaaaaggg ttacaacttc aatattccac
     9961 tggatttggc agatggaaca catgaggttg aaatcagagc atctgatgct tcaggtaatg
    10021 catccacttt gacaaggaca taccaggtgg atgtaacacc accggtattg actgctgaag
    10081 cggcaacaga caaaacaaac agggatagtg tagatattta tataagcact gatgaaactt
    10141 ccgaaatctg cattaacggg gaatatgcag gacatttcta tggcaacaat atcataccgt
    10201 ttacattgaa agatggctcg gaaaacgaaa tcagtgtaac tgctaaagat gcatttggga
    10261 attcatcgga agctgaaact attaaggtaa caagggactc gactgcgcct tctataacaa
    10321 tttccggtgt agtcaataat gcaatttaca gcaccgaccg tgaaattgtc gtttcagtca
    10381 ctgatactca tgattgcgag gcagttatca ctatggatgg ggaagcttat atacccggaa
    10441 tatatacggt agaaggaaag cataccttaa gagttgatgc cgcagattct ttcggaaaca
    10501 cctccagcag tgtgataact ttcactattg ataagagtgt gccgtttatt aaaatatcag
    10561 gtgctgaaaa tggtggtgta tataattcaa acaaagtaat tgaaattagt gttgaaaatg
    10621 ctgatgagat aacagttgaa atatctaccg acgggcaggc tgcacaaagc attccaatgg
    10681 agttggcggg tggaagcatt ccattaggag catacggaga gaagcatgat tataaaataa
    10741 ctgtaacagc taaaaggact actgctgaga gaacgcttat ttcagttgat attattactt
    10801 tgacaattga cagaaaagaa ccgcctgctg ttacaccaga cgatgataca ggtgatacag
    10861 gtgatacagg tggaggtaca ataccggtta ctcctgtaac tccggtaacg cctaaacctg
    10921 atattcctga aaatccagag actgataccc ctgaaactcc tgaacaggtt gttcctgcgg
    10981 tttctgaacc ggttaacggt attattactg tagaacctgt tttaacagga aacacagcaa
    11041 attctgctgt tacaatggag gtaattgata ctgcattcaa acagttgaaa gcaaatgctg
    11101 tgggtgttaa aactattggt atagaggtaa aagaactttc agacgccaaa acatataccc
    11161 aggagatacc tgcaagcata atgcaagccg gaagtgcaaa acggaacatt gcaatatcta
    11221 tgccgtatgc aacagtgact ataccgggca acatgtttac tgctgccgat attggcaata
    11281 aaaaaacaat tgaaatatct gtatcaacag tagatattga tgagcttgcc ggggaaacgg
    11341 gtaagaatat tgataaggat atcattaatg ctataggaaa caagcctgta atcagtttga
    11401 gtgcttcaat tgatgggaag aatgttgcat ggaataatcc tgatgcgcct gtagagatat
    11461 ctatccctta cacaccttct tctgaagagc ttattaataa cgaacatatt gtagtatggt
    11521 atattgacgg gaatggtaaa gtagttgcag tacccagcgg aagatatgat gtacaaaccg
    11581 gcagggtaac atttacaact acccacttca gcaaatatgc tgtaagttat gtaaagaaga
    11641 ctttcagtga catatctgac tatagctggg ctaaaaagca aatagaagtt cttgcttcaa
    11701 aaggtgttat aaatggcaag ccgggaggcg tctttgatcc cggaagcaat ataaccaggg
    11761 cggacttcat cctgcttcta gtcaaggcat tgggatttac agcaaaggca gacaccaact
    11821 ttgcagatgt taaggcttcc gactactatt atgaggcggt aggcatagca aagaaacttg
    11881 gtataactca aggtaccggt aataatttgt tcaagcctaa tgagtttatt acaagacagg
    11941 atatgatggt acttgtaaat agggctcttc tgttgcagga caagctctct gcgccaggca
    12001 atgtgtccga tctgtcaaaa tttggtgatg ttggtaagat tgcggcatac gcaaaagaaa
    12061 gcatagctaa tcttgtaaat gaaggaatta tactgggaag caatagtaat attaaccctc
    12121 ttgggaatgc attaagagct gaaattgcag tagttattta caggatttac tacaagtagt
    12181 gaaacttagt gctaatactg tgttagcgtt agttaacaca gtgaaaaata gagccatagc
    12241 aaacttagtt ttgctgtggc tctatttgct caaaggtaat ttgtttaagg gtataaggaa
    12301 acaaattctt ctgctgatac atacaagggt gcattttttt caaatataga aaatgcaccc
    12361 tctatacaaa aaacatattt attagtatta tctggtatgt tataatttat atatggtata
    12421 gaataaaaaa aatagcagta taattaatta tttttcttat gcaaatatat gcaaatataa
    12481 aataaaagca aaaatatact gatttataca tggatgaaaa gtatgtaaaa aatgccaact
    12541 tggacttgat agtaatgata aaattactga aattatatag gcatccaaaa taaattaata
    12601 acaagcatat taaatagttt tgatataagc gattgattaa tattgcttga tatgaatagg
    12661 agttattcta tgtataaaaa aaagatactt atcattgatg atactgagct aatagtgaaa
    12721 ataataaaag atatccttga gactgcagat tataatgtta tttcggcaag cagcggagaa
    12781 gaaggtttaa tcaaggtaga acaggaaaag cctgaccttg ttatactgga tgtcgttctt
    12841 cccggaatgg atggctttca agtttgcaga atgctgagaa atgatgacag gaacaacctt
    12901 atgcctatta ttattcttac gtcgcaatgt gatgaagatg ataagcttac aggacttgaa
    12961 cttggtgccg atgactatat tattaagcct tttaatccaa gagaactctt aagcagagta
    13021 agaaatacac ttgtaagaat tgatagaaat agatatgtta atcctcttac aggacttcat
    13081 ggaaatattg aaatacagac agaaatgaat caaaaaatcg ccagtaatga aattttctct
    13141 ttaatttatg cggatataga taattttaaa gcttataatg atgtatatgg ttttgctaac
    13201 ggagatatag ctattaaact tacagctgat attttaaact ataatatgcg gttatttggt
    13261 acacctggta attttttagg acacattggg ggagatgatt tcattattat aacaatgccg
    13321 caatgtgttg atgacatatg caaaggtatt attcacgatt ttgatattaa gatcttgaat
    13381 ctatataatg aagaagaccg aaaaaacggt tatataataa ctactaacag acggggagaa
    13441 cctagggaat ttccaataat gactatttct atggctgttg tttcaaatga atatagggac
    13501 ttcaaaactt actaccatat agctgaagtg gctgcagaaa taaaaaaggt tgtaaaatcc
    13561 atgaatggca gttgctactc taaggatagg cgaagatgtt aatgtgaata gatttaatgg
    13621 aaacacatat gattcatagc acatgtgacg aggaatataa aaacaggtgc tgcaggggtc
    13681 tggggtttgc cgatttacca cttgtgttag tatttaccaa tttacgattt gcggacaggt
    13741 ttgacaaact atatcaacgg gtgtataata tgcataatat ggcacattgc catataaaac
    13801 tgcataaaaa aaacatggta aaaaagctga atcccctttt agaaaagggg tacggaggaa
    13861 ccataccatg gggggttaat ccactaatgt gattcacaat tatttaatgt tgctggcaga
    13921 ataatttata ttgctgccgg tgatgtagct aatgtgattg aggaaggtgg tagggtgtcc
    13981 tattcccgaa cccgtcagct aactccggaa gcttaaatag gaggacagca tgcacaaagt
    14041 gtacagacta gagaaattta ttaacgcaaa aataccgttg agtgtttttg caatggttct
    14101 gctttttgcc cttttctcac ccattatata cgctgaatca gctaaagagg gaatagtaaa
    14161 cacagaatgt ctgaatgtaa gacaatcccc atgcacttca gcagcaatcg cagcccagct
    14221 gttaaaggat actaagattg aaatcgatga attccaggaa ggctggtata agattacata
    14281 tggaaatata aaagggtggg tacatgcaga ttatgttact acggaacaga caatgacggt
    14341 aactgaaaat gcaggttcaa acggttcatc aattaacgga gcttctaatg gtgaacctca
    14401 gaaaacggga gtaattacag caaacgtact caatgtaagg gaatctgcgg gaacatcatc
    14461 gaaagttgca gcacaactgg aaaaaggtat ggacgttaat gttattgaaa caaataacag
    14521 caataattcg gaaacctggc acaaagtaag ctatggcaag attacaggtt ggatttctgg
    14581 cgagtacata tctttcaagt atgaacccat agatgctgga gttgtagatg ttgacgttgc
    14641 caacgtaaga actgcaccgg atatttcttc aaaaattgcc acgcagatga caaaaggaca
    14701 gaaactggat atctatgcct ggtccgggga atggtataag attaaactgc aggatgaaaa
    14761 ctttgcatgg atatttaatg aacttgtatc tacaagaaat tcttttgctt cacgggaaag
    14821 tactggagca agaggacaat ccagtgttgt agataggtct ctggacaaga gacagcaaat
    14881 tgtaagcttg gccaagaaat atttaggagt taaatatgtt tggggaggga caagcccaaa
    14941 aggctttgac tgctcaggtc ttgtccaata tgtgtacaag cagataggta ttactcttaa
    15001 ccgtgtagca gctgatcagg caacacaggg gaccaaagta agcagagctg aactgaaacc
    15061 cggtgatctt gtattcttca atacaagctc aggcagcagc atagaccatg taggcattta
    15121 catcggagga ggcaattttc tgcatgcagc taatggaaga ggtaaggtat taatcgatcc
    15181 ccttgaccac agttattata aaaacagaat gataactgca agaaggataa taaaataggg
    15241 ctttaataac tggcagtttt agttatgttt tctgtagaca ttttttcaaa taggacattt
    15301 gtaattcgta aacctataga atactaagtg tagactgcaa atattaaaca aaggatagaa
    15361 agcagactta ttattaaata ttaattttag caattacgct gaaagtaaca gctttttgca
    15421 atatagcatc ccaaaccggg atgctttttc atttgtgctt catttgtgct ttatttgcac
    15481 ttatgaaata ttatatatgc gtttcctaaa tatcatatgg gtattataaa aatttatatg
    15541 gaaattatta taaaaatagt atataatatc aaatgttatt acagattttt ggtggaacac
    15601 aagctcgtta ttttaataga gatatagttc acctatacgc atgtgcatat ttttgtgcat
    15661 gtgcatattt ttgaacagga atggagctta aatatgaaaa ggcccctggt tttatttgct
    15721 atagccctag ctataggaat attgtttgca aaaattacac aatcatataa atttatagta
    15781 atttcagcat tgatccttct aatggtaata atcattattg aagttaaggt cgtaaaaaac
    15841 cgtatgatta tattgccgct gacaggtatt ttgctctttt attcaatagg aggatttgag
    15901 tttttatata atgatagaat aataagtgaa aagtatcagg aatatgccgg agagcaggta
    15961 acaataaaag gagtaatcga ctccgagcct gaattccagg ggaaaaaaat aacatgtgta
    16021 gtctattgca aagaaatcat taataatgac aaaaacaaaa ctaaccggaa taatgctgat
    16081 aaatttatta ctggtaaaat atccctgacc atcctgacct ctaacttaca aactgcacca
    16141 gctgcatcag ctgtacaaac tgcaccagct acaccagttg catcagttgc acaaactaca
    16201 gaggcaatgc tcattgatta cggcaaaaca atagaaataa caggtacact aaatattcca
    16261 gcaggaaaaa gaaaccctgg tggcttcaat taccaaagct accttgcagg ctcaggcata
    16321 tctgcaacaa tgtatgtaaa ggagaacagc atcagggttg gggaagagtc acatgcaaat
    16381 atctttttaa agattggaca ccttatacga aaaaggttta taaatgttat aaataacagc
    16441 atgcctccaa aacaggcagg gcttatgaac ggcatgctta ccggttaccg tggaggaatc
    16501 tcggaagaca caaaagcagt tttcagtgat tcggggttaa tgcacataat agcagtttcc
    16561 ggcatgcatg taggatttat tatgctgcct tttatttttc tgtttaaaaa gatgggagta
    16621 aagaaaatag tatcaaatcc tctcattatt ttaatcttaa tactttatgt atctataaca
    16681 ggattgcagc cttcagtgct tagggctgtt ataatggctt caacagtgct gctaagccaa
    16741 attatatgga aagagcctga tacccttgca agtatatcct ttgcagcaat actcctgtta
    16801 atatggaatc cgcacatact attcaatata ggttttcaaa tgtcttttat tgctacatta
    16861 tcaatagttc tgcttagaaa aaatgtatca gaaataatta agcctgctgc aaaatctgca
    16921 gtcaaataca taacaagatc tacaaaagca ttgtctgccc attttgttgc tcattctgat
    16981 aatcattctg ataatcattc ggttacgcgt tctattgccg attctatgcc tgtttattcc
    17041 caatattcca ctaacaaaag caaaccagcc tccaaaatta aagacaggac tgagaaatta
    17101 gccaaaaaag tgacggatat acttggaact actctttcag cccaagcagg aatattgcct
    17161 ttatccattt tgtattttaa taaagtttct attatatcgg ttgtatcaaa cctaatggta
    17221 gctcctgtaa cggggactgt aattgtactg ggcttattca tggcaataat cggacagata
    17281 agcctatttg cagcgaagat tgcaggatat gccaataaca tacttctaag ctttataatt
    17341 tttatatctg aatttactgc ccaaatacct tttgcaattt tgaaaaccgg aatatcttcc
    17401 ataatgctta ttctcgtgta ttacatatta gtatggtttt ttctttggct aaaacccagg
    17461 cacaaaatta aggtaaaacc tatgtcatat gcatgggctg caatatgtgt tatattagtc
    17521 ataagtacga taaatctaat tcccgggcag cttgaagttg tttttcttga tgtaggagca
    17581 ggagattcaa tatttataag aactcatgga ggaagaacag tactgattga tggtggtggt
    17641 gaaaaaggca agctggataa tgaacctgat actggtgaaa aaatcgttgt tccttttctg
    17701 ttgggttacg gggtgaagaa attggatctg gtagtggcat cccatgggca tggagaccat
    17761 attcagggct taatgcctgt attgaaagaa tttaatgtag gtaaggttat tctacctgac
    17821 tatattgaag gtaatgagtt tgataaaata ttaacaatct gtggagaaag gaaaataaat
    17881 gtaacatatt gcagccaggg tgatactatt aaacttgata aaaagacgta ttttgaagta
    17941 ttaaatccac cccgtaatat tgtacctgaa attaaaatgt ctttaaataa tagttcttta
    18001 gtgttaaaat tatactataa gaatgtaact atgctattca ctggggatat tgaaaaagag
    18061 attgaggaaa ttcttgcaga taacctgctg gaatcaggtg tccatgtgtt taaagttcca
    18121 caccatggtt cggaaacttc ttcaatcgga aagtttcttg aacttgtaaa gccccaagtg
    18181 gcggtaatca gtacaggaag aaataattat ggtcacccgg ccccctctgt tttgaaaaga
    18241 cttgaggaaa caggcactgg aatttacagg acggatttaa atggagctgt tataattaga
    18301 acaaacggga gattaataaa agttaaaaca atgctgaatg gtgagcaagg ctaaggagat
    18361 attcaagaag gttaaggtgg tgctggataa tgagtattga tattttaaaa acacaattaa
    18421 aaaataataa tataaaatcc ctataccttt tttatgggcc ggaggaattc cttaaaaagt
    18481 tttacataaa aagtatagaa gatcaaatag ttgacaaaaa tatggctgca ctaaataagg
    18541 ttactctggc agataaagta gatgtacaaa aaataataga caactgtgat acccttcctg
    18601 ttttttcaga aagaaaactg gtaatagtga aaaactcagg gctgttcaag ggaggttcag
    18661 ggggttcgga agggaaaaaa ggcggccgga acggggacgc actgcttgat tatattaaaa
    18721 atattccgtc gtatacatgc ctcatttttt atgaagatga aatagacggg cgccttaagg
    18781 tatctaaaga aataaaaagt cagggacttg ttgtggagtt tccgtatcaa aaacctgttg
    18841 atcttgtaaa atgggtttat aaggtttcaa aaacatataa aaaagaaata gatgcagaag
    18901 cagctgcctt attggtggaa ggatgcgaat caggtatgac gggaatactc aatgaaatag
    18961 aaaaactggt ggcatataca ggagacagga acaatattac tttagaggat gttaaaaagg
    19021 tatccagtgc tacagtaaaa agcataattt ttgatttgac caacgctatt gtggaaaagg
    19081 acatggcagc ctcattaaag ctcctggatg atatgattgt ccttaaagaa ccggctccca
    19141 aaataatttt tatgattgca cgccagttta ggcaaatgct tcaggtaaaa attttactgg
    19201 aaagcggcaa cagcccaaag gagatatctt ccaggctaaa actgcagcca tttattgttg
    19261 agaaactaag aaaaatttct gcaaatttta atataaaacg acttaaagaa aatatggaag
    19321 agatatttga atgcgatctt gcaataaaaa cagggaaaat gaaggacaga acggcagtgg
    19381 aactgctaat agcaaaactt acaatctaat aggaacatac taattccaaa cctaactcaa
    19441 acttggtctt aaacagtaaa aaaacgtact caggtacgtt ttttatcatt ttatcgccat
    19501 tatttactta tcgctgtatt ataagccttt gtcaattttg atttctgtct tgaagcattg
    19561 ttcttatgaa ttacattctt tactacagct ttatcaagaa gttttactgc ttcctttagc
    19621 atttcagctg catgaggatc tttattatca atagctgttc tgctcttttt aacagttgat
    19681 ctaagagctg atttcctcat tttatttgcc aatgcttttt tatctgcaat ttttatacgc
    19741 tttatagcct gtttattatt agccaagtat taacacctcc agttgattgg tcaacgcagt
    19801 aaattttatc atgaaaaatt aaaaattgca aggggtcatg gaaaacaaat tataatattt
    19861 gccttaaaat taggcaaaat aataaaacaa atataatatt ttatatattg gaaaggattg
    19921 ataactatga ttgataaacc agaaagaaga agcgttgtta cagacctggc tattgaagcc
    19981 catgaacttg ttaaagagca gactgtaaga ggtgcccagc aaaaggaaga taatataccc
    20041 ggagttgatg taaccaatgc tggaacagaa gatataaaaa tatcaagggt aaagataagt
    20101 tctgtacagg ggcagaatgc gcttggaaag cccatgggta cctacattac ccttgatata
    20161 ccaggaatta aggaaaatga tatagagtta tatgaaaatg cctgtaaagc tttagcatca
    20221 gaacttgcac aactgctgcg actaaatgaa aaatcaacaa cccttgtagt aggactggga
    20281 aactggaatg taacctctga ttcacttggc ccgaaagttg tatcaaagat gatgataacc
    20341 agacacttgc ttcaatatgt acctgaacag gttgatcagg gcgtgagacc tgtatgcggc
    20401 atagctcctg gtgtactggg tatcacagga atagagacag gcgaaatagt tagaggaata
    20461 gtagacaggc tcaggccgga ttgtattatt gcaattgatg ctcttgcctc tagaaagctt
    20521 gatagagtta acacaacaat acagattgca gataccggga tttctcctgg ttcaggggta
    20581 ggaaataaga ggatggatat ctcccgtgaa acccttggtg tccctgttat tgccataggt
    20641 gttcctacag ttgttgatgc agctacaata gcaaatgaca caatagacaa ggtaattgac
    20701 agttttatac aaaaagctga tgaagggacg gaattctata ataaattaaa aaacatggac
    20761 agggatgaaa aatatcaaat gatacaggaa attcttgatc cttatacagg taatttaatt
    20821 gttacaccaa aggaaattga cgaggttgtt gaaagaatag caaaagtaat agccaacgga
    20881 ttaaatatag cactgcatca gggaattact ctggatgatg tagacaggta tttacagtaa
    20941 gaaagctgct gattttagtg ctgtttttgt cgtgcctgat acgacacttt gaatatggta
    21001 tttacacctg caatttgaaa atttgaaaaa aattatgctg aattcttgac agagttaaaa
    21061 tgattgattt ataattgtat ataaaataaa tatatgtcgg atgattggtg ggggagagat
    21121 attccaaatg gaatgcaccg aaggagtagc actctcaggt aaagagacct cactgggacg
    21181 aatctctgga gagaccatta ttggcgccga aggggcaagg cttaaatgct aatctctcag
    21241 gcaaaaggac agagcaggat aaatacacgc atcttacttt gttattcaga ggtcatgtcc
    21301 gctatggatc tgacctttat attttatgaa aagaagttta cgggagggtt gtattgatga
    21361 aggttatagt aacagacaaa atggctgatg atggaattag gtatctgagg gagaacggtt
    21421 ttgaagttga tacaccattt ggtatttccc ctgaagaatt gctggaagta atagaaaatt
    21481 atgatgccat tatagtaaga agtgcaacaa aggtaacaga aaaagttatt gaaagagcaa
    21541 aaaatcttaa ggttgtaggc cgtgcaggaa acggaataga caatatagat gtaaatgcct
    21601 gcaccaaaag aggtattctt gtagtaaaca ccccggaagg caatattatg gcagcagccg
    21661 aactaacaat aggtatgata ttcgcactgt tcaggaatat cccccaggca tacatggctg
    21721 caaaaaacaa ggatttcaga agaaacaaat ttgttggtaa cgaacttgat ggaaaagttg
    21781 caggagttat tggccttgga agaataggta ctattgttgc aacaaagctt aaagctctaa
    21841 acatgaaagt tataggctat gatccattcg ttacagatga aagatgccag aagatgggca
    21901 taaagaaatg tgaaactatg gaagacttac taaaggaagc cgaccttgtt actgtgcaca
    21961 tcccaaaaac tgataaaagt cagggcctta tatcagaaga agaattgaag ctctgtaaaa
    22021 aaggcgtacg tattattaat gttgccagag gcgggattgt aaacgaaaag gctctatata
    22081 acgcattaaa ggaaggtcat gtggcaggtg ccgctataga cgtatttgaa aaggaaccca
    22141 acttcaacaa aaaaccggaa gaacaggatt acagcaatcc tttacttgaa cttgacaatg
    22201 taatatacac acctcatctt ggcgcatcaa gtgaagaggc aaatcataac gtaagcagcc
    22261 aggtagctga acttatagca ggtgccttaa gaggcgaaat ggtagcggcg gttaatatgc
    22321 ctccaatgaa ggtggataat ctgaatggcc tgaaaccgta tgtagaaatc gctgaaatat
    22381 tgggtaaaat ttactatcag gctgagaagc ccacagtcaa taaagttgaa gttatatata
    22441 gcggagatct ggcagaaaaa gataccagac taatatctct ttcagcactg aaaggcttgc
    22501 tggaacatgc tgtaaaagaa aacatcaatt atgtaaacgc aggttatatt gcaaaaacaa
    22561 tgggcataga aatggttgaa agcaaaagct catatctgga taaatacacc aaccttatta
    22621 cattgaaatt tacgactaaa gacagggaac ttagtgtatc cggtacagtg tttaccaaag
    22681 atgaaataag gatagtggat ttcttcggtt acaagctgga ttttgagcct actccatacg
    22741 taatagccat acagaatatt gataaacccg gtattatcgg tcagataggt accatactgg
    22801 gcgctaatga tatcaacatt gcagccatgc agtggagcag aaacagaaaa ggcgagaagg
    22861 ctgtagcctt tgtaagtgta gactgtgaag tatgcgaaag catattaaac cagttgagac
    22921 aaattaatgg agtattaaaa gcaacaatgc tgaagtttta agatatttta aatagcagca
    22981 ataagcagca ataagggaca tttctcaagt tgaaaacgat aattgagggt tgtcccttat
    23041 tacataatgg ctttgcacat aaaagtttat tttttggtta acttttgaga ataatagtat
    23101 aaaaggacga tgtttctatg atcggttttc tagtatttat aattggatta gtgttcggaa
    23161 gctttatgaa tgtatgcata taccgcatac ctgcacatga atctgtggtt acaggtccat
    23221 ctcattgtcc tgcatgcggg aaacaaataa aatggtatga cctggtaccg gtattaagtt
    23281 ttcttatact tagaggcagg tgcaggtttt gcaaaacatc aatatcacca cgatatccgg
    23341 cagtcgaatt gcttaatgga acaatttgga tgctgctcta ttgttttttt ggtttgacac
    23401 ctatattcat tattctatgc atagtatttt catgtgttat ggtttatggc tttataaagt
    23461 atgatgggtc aaaaagtgcg agataatgta attattttca tataaagatg ctattcattt
    23521 aattctcaca ttgcgcacaa aactgaattt aaactggaaa atgcaaaaat attgtcaaaa
    23581 aattaaaaaa tacctgttta ttttttataa taaatggtat aatcaaatca tgaaacttat
    23641 tatgtgatgt acttatataa tgcattacat agaaaatagg ggaaaatata aaaaaatata
    23701 ttgggggtaa aattatgatt aaaaaaatgt taaaatcaat ggggaaaaac aaaaaaggtt
    23761 ttacactggt ggaactgatg gtagtagttg taattattgg gatactgact gctattgcgg
    23821 tgccggtgta taataatgtg acgaaaagtg ccacagataa tgctaacgcg gctaatatac
    23881 gaattcttga aggcgcgatt tctatgtatt taacgaacaa tcctagtgat aatgttacat
    23941 ttgaagatat tataatggat aatactggca ctctttctgt gccatcgggc aagactttaa
    24001 atgggggtaa aaaattagta ccagaatatg ttacttctat gcccaaacaa ccatcaagta
    24061 cattagggta tgtaaagaca aatggagcag gaaaacgtgt ggaattagag gtatcagctg
    24121 caccagaggg ttaaaaacaa ccttctatga agtgttaaga aataaatttt ataacttatg
    24181 tgcagaatta actgtttcat agcatgtaaa gggacagcat atgaaatcat ccaaaagaaa
    24241 ttttttacta agaaataata ttataaaaaa atgctaggtt tgctattttt gagtttatgg
    24301 tagtttttat tatattaagt gtattagtag ctttagcatt aaggatttat agtacgattg
    24361 aaactgactt agtcaaaaaa atactagaaa ataataaact cctattacta aacgccttac
    24421 aaatctactt aattgaagtt ggcataccgg aaggtggcaa agcccctccc gggggctgga
    24481 aacaagccat aatgccatat cttcaggaat ggccttcaca ccctcttggt atacttggcg
    24541 actatgtaat atcaggaact taccctgatt acaaagtaga atttgttcct gaataaatta
    24601 tggaaaaagt actttatttt gtacatataa ttttgtaagc gaaatagcct tagataaaat
    24661 ctgaggcttt atctgtttgc ggggcatgtt gcccagccga tggtttgaaa gaaaacctgt
    24721 cgcccagtta gcgggaagac aagctgtaag caagggcgtc tctagtgatg gaggggtctg
    24781 aaggaagctg atggcggttg attggagcgg agcgcaagcg atccagtgtt ggcataattg
    24841 agcggataac cctgcagaga gtgggaaagt ccaaaggcta atttagctca taatgttagg
    24901 atggtcgttt caacaacagg cgaagcaact taacctcgga agtgctgtac tcgaagctgt
    24961 taaatccatc ttcaagcgaa agcaagaggt ggaggagggt gcagtatgca gatgacgtcg
    25021 tagtagtgaa taaacctaag cctgagaaag ctggtaacag actggaggat ataaacttag
    25081 gtgacatggg acatggtttc catgaccata gctactcaaa aggtggctat gggtgcgaag
    25141 gacggaagga attcaaagtg aattcaacaa ctgaaacaag ggactccgaa ggtgcttgaa
    25201 cgaaggctga aatgctggaa gaaacgaagg aacggcataa aagaacttgc tgtgaattac
    25261 gatggcacga acatgaagta cttggcggaa ggaagataga ttgccttgag ctagaaacgt
    25321 caacggaaac taaggaagaa catgtaggaa gaacaagtta cagggaaggg ataccacagg
    25381 accggaggtg gaacatgaag aaatggtaca gcctgattga taagatatat caacctgaaa
    25441 atttaacaaa agccttcaaa cgggtaaaac gaaaccaagg agccccagga atagatggtg
    25501 agacggttga agattttaac gttaagcttg aggagaatat tgagttcctt catgggaaac
    25561 tgaaaaccaa cacatatgaa ccaagcccgg taaagagagt agagatagag aaaccggatg
    25621 gaggcaaaag attgctaggc atacccacgg tcagtgcaaa ttgcacaaaa gttcaatacc
    25681 cttttgggaa atgccacgat tacacaccca tttcatccat tatgtggcca gggatatgaa
    25741 ttactgaaaa taaaggaggt gaatggaaca cgtttgtact cactacgaac ggattccggt
    25801 gtcatgtgtg ttcctgaatc ctggacagat cggaaagttc agccagaaaa cgttcaaaac
    25861 tcgcgtaaaa ttccctttga tgcattaact ttaagagaat tggcgcagct tattcaagct
    25921 attgaaggtt taccaataac agagaaaaaa agtgttgaca aagttaataa agagagataa
    25981 aatcttaaca tgaataacaa tgataagata acactaagga aagtacctac acgagaatta
    26041 aaggagcgta aagcacagat tttaagctcg ttgccaccaa tggatgacat tatacggggt
    26101 tctctcatca cacgttccat aaaatgtggc aagcccaatt gtcgatgtgc taccggtgat
    26161 gggcataaga gtttttatct gtcttcctac taccatggta atacctatat ggattatgtt
    26221 ccaaaatcgt gggaagcttg gataagtaaa ggtataaaaa attatggggt catacaggat
    26281 ctcctctcag aattaacgga aatcaacctg gaactgttca gacgcagagg aaagaaataa
    26341 tcctttgtat accctaaatg aaagggtggc tatgatgtcc agttcaacat taattgcttt
    26401 ggtaggaaat gatgtccaca aggcagacga acttgttaca gtttttgcgg ggttggttct
    26461 ttctttcctt caagcaatgc ataaggaggg agagagtaat ggaaacatca tcgaaagttc
    26521 ggacggaaca tctgaatcga atggcaatag tgtacattcg acagtcgact ctgactcagg
    26581 tacgattcaa cagggagagc acggagcgcc agtatgcgct tcaggagaag gcattaagtc
    26641 tagggtggtc acaggagcag atacaggtga tagacgaaga tctagggatc tcgggatcag
    26701 gtcgttctca gcgccaggga ttccagcaac ttgttgcaaa ggtttccctt ggagaagttg
    26761 gaggaatttt tggtctggaa gtttcacgtc tggcacgctc ttcggcggat ctatcaaggc
    26821 ttctggaact ttgcggccta tttggcacaa tcgtagtgga tgaagacggt atctatgacc
    26881 tgagcgattt taatgatcgc ctcatattag ggttcaaggg tacaatgagt gaagcagaac
    26941 ttcattttct tagaactcgt atgcttggag gaaaaaagaa caaggcaaaa aagggagaat
    27001 tacgttttcc tttacccgta gggtatgtat acgatgctga tggtcaaaca gttatggatc
    27061 cggacgaaga agttcaaata gccgtacata atgtttttaa agctttcaaa tccagcggca
    27121 gcgcttatgg tgtagtccag tattttgtgc aaaacaacct tcagtttccc aaaagggcat
    27181 atggaggggc atgggcagga aagcttgttt gggggaggct tacacatagc cgggtacttg
    27241 gaatattata caatccagct tatgcaggtg catatgtata cgggaggtat cgtgatcaaa
    27301 aacaggttaa tcctcaagga ttgtttattc accatacagt ccgcttgccc aaagagaagt
    27361 gggaagtttt tattccagac caccatacat gctatattac atgggaaatg tatgaggaaa
    27421 accataagca attacaaaac aaccgtacca atattgagaa aagtggtcca gcccgggaag
    27481 gtattgcatt gctgcaaggg ctaatactat gtggaaaatg cgggcggcgt atgacagttc
    27541 gatacactgg taatggcggt attgctcctg tatatgaatg caagggaaga tgggagcatg
    27601 gtgtaaaagc cacatgtact acagtttctg ctccaattat tgataaagca gtatcaaata
    27661 gacttatgca ggttatgcag ccggcagaac tggagcttgc cattgaggtt atggataaat
    27721 tattggaaga ggcatatgat gcagacaaag gatggaagct gtcactagag cgtgcaaagt
    27781 atgaggtaga tcgtgctgaa cgccagtacc aactggtaga acctgaaaac cgtttagttg
    27841 tgcgaagcct ggagtcccga tggaacgaaa aactggcaga gttaacacga attgaagagg
    27901 aatatgcaaa acaccgctcc cggcaaagtt ggcgacctac agagcaagat aaaaatatga
    27961 tcctttcttt agcaaaagag ctgcctcgta tctggaatgc acaaaccact acagctaaag
    28021 aaaagaaacg aattctacgt gtccttatag aagatattac agtgtttgca gagccaagac
    28081 aacctaatgt ccgactaggg cttagatgga ggaatcactg cagtgaggaa atttatacaa
    28141 cgaaacctct gccaaaagga acagctc
//
LOCUS       DAOSDD010000025        27210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_816_length_27210_cov_5.220733, whole genome shotgun sequence.
ACCESSION   DAOSDD010000025 DAOSDD010000000
VERSION     DAOSDD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 27210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27210
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_816_length_27210_cov_5.220733"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(484..6129)
                     /locus_tag="PK733_03950"
     CDS             complement(484..6129)
                     /locus_tag="PK733_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ09729.1"
                     /translation="MFFYSNRKRKLYANITIVMFVLYMLIGVILPVSKVHADSYSHAD
                     DGMIMFHGPKNAHNLLVTELNWGDDSEPGKIVAEADILPIKNPSDSWTPSLRFYWSES
                     KWVSIGISTDGKFLTYNAGRFGNAPLGKWYTVRMVLENGKVEMWGGEKGTELNLLTTF
                     NAPVSEAPSHLIIGKGFGNGTPTYPNPFTANDASPLGEQGLIYMDNVRLWADDQLIIE
                     DDFGQGVDLSRWTIYMSDKIQPAALIEVYYDAEGEITDISDEVIFVNRKFIVPESVAE
                     FTFKDGNKYMKVVFNSDEWVIKEVKQDEPDDCSHVDDGLVMFLGPENAHNLLVTELNW
                     GNNSEPGKIVAEADILPITNPGASWTPSLKLYWSESKWVSIGIDSYKKQFIIYGASGR
                     FGNTPLGKWYTVRMVLENGKLEMWGGEKGTELGLLTTLNAPSSEPPSHLIIGKGFGNG
                     TPTYPNPFTANDVSPLGEQGLTYMDNVSLWADEQLIIEDDFEQGVDLSRWTIYMSDKI
                     QPVLLVKVHYNAEGEVTDISDEVIFVNGKFIVPEGISEFAFRDGDKYMKAVLANDEWT
                     ISEAEKIEHIYVSPEGNDDNLGTIDQPFATINKAAKAATPGTTVHVLPGIYTEPIIIN
                     KSGTADARVRFVSEERWGAKINVARQGDKATAMLFYGDYIDIEGFEITGDCLYGINGD
                     GFNVRIIGNHIYDLAPKEDRLGGAAIVAHDSVEVIGNLVHNIGPEGEESNLIHGIYTV
                     GTGVKILNNITYSNAGWGIHAWHKATNTIISNNLIFGNRKGGVEIGSSDSTIIADNFV
                     VTNNIIIYNNNDGVIELGKTGPNNKYENNVVYGNVGKAFRLHDGKIDHNTLILDPQLV
                     DFKSDGSGDYHLTAISPCIGNGTKFGAPSIDYDGNERLADKAIDIGPFQFTGQLPIRV
                     KVPYEAEGDITESSNGVFFADGNFMVPENVTEFTFKDGYRVMSAELSNGEWVFEEAER
                     LLHIYVSPQGNDDNPGTKEYPFATINKAATAATPGATVHVLPGIYTDPIKINKGGTEE
                     AWIRLVSEEKWGAKINVVSRGSLPLTVEEGYVEIEGFEITGNDVLYGINVRESNVRII
                     SNHIYGIAPNGGPWGSAIVTYDSEAYKIHSVDIIGNLIHDIGPKDGSGGNIHGIYIAG
                     NNMKVINNISHSNSGFGIHTWNAARNCTISNNLVFKNGTGGIKVGAGETPVGIIADNF
                     IVTNNIVIHNGNVGIIEAGATGPNNKYENNLVYENTGNAFELQDGKADINTLTVDPKF
                     VDFKADGSGDYHLIESSPCIATGTKLGAPSVDFDGNARPSDKAVDIGPYQFTSEKPGE
                     PDLEEPTDPKDPEEPYEPEEPQGPTVPNEPAEPAKPSEETTPDDVEEPQESGEENEII
                     VQVKNIINEIKEGNTASVIDISLTDLAEDIYRKIINANNLVSLIDEPQIAIELSKSLI
                     DNSGILLMKLAVSNNYEAHDSISKELSKLVETVISIAGTLRVDFQAVEGRAEGEIILG
                     EVEDGKLVDEYTEKKLDLIVATAKQLKESLEKAGVEAEIKPMLYLNAVNGADNTASAE
                     LKIPANLIIAANEKGVAGVVVTTDVAEFTVPSDAIELKKDSVIVLNSEKIDNEALSGE
                     VKKSVGDAPIYDLSFTVDNERVAKFNKLIKVSIPYTLKDSEDAEKVTVFYINEKGELE
                     NIIGVYNNDTKRVYFETEHFSMYTVKVDNVRFNDVASTFWAANYIEVMASKGVIMGIG
                     GNFFAPDRNVTRAEFVAMVVREFKLFDSKAVNNFNDVNEKDWFYPEVTVAANLGIIQG
                     RPGGIFAPNDKITREEMAKILSNVLTGIMGKKMPANKTAYIDVFKDKEIIADYAKDAV
                     AMGSRYKFFTGRPDGTFAPKDNANRAEASKLIYMLFYIK"
     gene            complement(6401..8002)
                     /locus_tag="PK733_03955"
     CDS             complement(6401..8002)
                     /locus_tag="PK733_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09730.1"
                     /translation="MKRKTIAIFLAVVMIIGITAGCAGTKRSPDSSNKEKTTSNTAEN
                     TVSELEQFSPMTFTTVTTSQEAASNPNTDLFAKALREKFNITLKIDYIAQSSLAETMK
                     LRMAGNEDPDVHFNLFGDFDKYGVDGYFIAFDDYFDKLPNYRAQWPDEFWQEYLELAT
                     APDGKYYSLRVPMGNVQLVGEGWLFRMSTIEELGVKAPETTQELYSYLKAIKNKYPDS
                     IPISNRNGLWGLTRRFEDAFRTSSIFQYDGDYNKFVYEPITDKYRDMLIYLNKLYKED
                     LISKEFSTQSAQQWSEHVGQGKVHVLLEYFAREGWANNLCTEVDPKANWLSSPKFINA
                     YPDKKLMVSRYSAVGASSARISSRNSQERIDRLIKVFDWLATEEGMIKTQMGEEGVTF
                     KYNSNNEIEYMDNIITPLNPDKGTDTLLKYNLEYRIINYPRFFYYQGGGPSTVIGDLV
                     RKNNYDYFKPYSLVFNDAEKATIADFGTVVNDKMAEYTLKFIMSQLDPTDNATWKTYV
                     ETINKNGLEKILPVYQKAYEEKYGK"
     gene            complement(8080..9006)
                     /locus_tag="PK733_03960"
     CDS             complement(8080..9006)
                     /locus_tag="PK733_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09731.1"
                     /translation="MVKDNTTGSKILDIIIHIILIFVVVITLYPVLNIAATSLSSSVA
                     VEKNLVTFYPKELTFKAYELIFKNDIIPNSFRNSIFYTVVGTIISLVMTILLAYPLSK
                     KDLPLRRFYTVFLVITMYFSGGLIPSFLLIKRLGLYDSVWALLLPGAISTYNTMIMIS
                     FFKSLPRELEESAYLDGANDLIILIKIIIPLSTASIATIGLFYAVGKWNDWFTGMIYL
                     KSSRKYPLPLILRELIIENKLEDMMDVGGVASNLNAGKSMNVMKDDAQKMVNADTLKY
                     ATLFVSMIPMLIIYPFVQKYFVKGVMIGSLKG"
     gene            complement(9115..10065)
                     /locus_tag="PK733_03965"
     CDS             complement(9115..10065)
                     /locus_tag="PK733_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09732.1"
                     /translation="MDAVKHKKVIVDFRWNTLWKEIKKTKYLQLMLIPAIIWYIIFCY
                     GPMYGVTIAFKDFNMRIGILNSPFVGFKHFERFITHPYFWKLMRNTVLLSCYSLIFSF
                     PIPIVFALLLNEVRNIKFKKFVQTVSYLPHFISTVAIVGMIVMMLSPSSGFINKLLTS
                     WFEIEPIYFMSEQCWFRPIYIISGIWQSTGWNAIIYLAALSSIDQELYEAISIDGGGR
                     FRKIWHITLPGIKPTIVILFILGLGSMLSVGSEKIILMYSPITYEVADVVSTFVYRVG
                     LFDAQYSYSTAVGLMNSIVNVILLTIANRMARKFADFSLW"
     gene            10332..12602
                     /locus_tag="PK733_03970"
     CDS             10332..12602
                     /locus_tag="PK733_03970"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09733.1"
                     /translation="MEKNNKKHSKILKRFLFSYSVIIVLPLLVGLISYKESATIISND
                     TKNTHLSMLRQSMEIMDRITGETDRIACQMAVNPQVIKFCYAYDFNDKSIYYNMWETL
                     SYIKPYSAYENAGSEYNIYFNKSDMVFNQNGALTFDEYYKHFGYQNIDIDEFRQRFLN
                     IFNKGTFISSETVKSTEITTTSKKISNYNVITYFKTMPLMNTGILNAVLFVRIKEDSI
                     LKFLRGLDVSDDGCVYILDENSEIITGIPAGLGKSVPYNIDFIGSEGFVEKNINGKTM
                     YIVYTVSSYNGWKYVSAVPAHIVMAKVNSIRNLILGITVVSLIIGIILLILLAYANSK
                     PIIELKKTLLDFFNSEDDNKKDEFDFLKSSISDLIHQSSAMKSDIQKQRSTLQKSVIE
                     RLLLGRNIPSSILDWASQAGVELGAQAYTCVLIGLKNVQIENDQLQEFNIGQIIHNQT
                     GQKGYLIDINPNTLCLLLCLNTNEEQKCKQHTERVISWISNQIQENLNIIPTFTIGSI
                     YYDLSDIYHAYNEAKQALDFMPIREPNTILWYSSLEMDAKSSYYYPIEVEQRLIHLVK
                     YGYKDDLSNLLSTILEKNLENPALLHKSQMLLRHEINGTIYKLNIGNPEDVVAELDNC
                     ESLNDVFKCMQTVLNNMCDDVNEQKNNRNIKLVDEIIAYMESNFSDSGLCLSQVATEF
                     NLSEGYISQLFKEHTGEYFAAYLENIRLEHACQLLERSSYTINEVALRSGYNSPQSFR
                     RAFKRVNGFNPSDYKV"
     gene            complement(12661..12828)
                     /locus_tag="PK733_03975"
     CDS             complement(12661..12828)
                     /locus_tag="PK733_03975"
                     /inference="COORDINATES: protein motif:HMM:NF036481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09734.1"
                     /translation="MEYENIKQTKLIYRPNRFVAHIEIDGKEEVWHVKNTGCCKTLKL
                     LPLIHWLKKAP"
     gene            complement(12945..13112)
                     /locus_tag="PK733_03980"
     CDS             complement(12945..13112)
                     /locus_tag="PK733_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09735.1"
                     /translation="MGTTVLSTQNNNMPVTEIAKKAGKSVVGIRMTVLSTSSRFNQYT
                     TQSQEEEVPVL"
     gene            13348..13728
                     /locus_tag="PK733_03985"
     CDS             13348..13728
                     /locus_tag="PK733_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09736.1"
                     /translation="MEIRENIMVPLGYGKFVRSDKIVSLEPIEDERGPGRRTTVYIED
                     VKSPLIASRTENSILANMVEVPKEELEAAAALELLKDIRDDILHIGPMLRKSIKKEAS
                     FDIDKIEKRINEILKHEIEYDNSF"
     gene            complement(13983..14240)
                     /locus_tag="PK733_03990"
     CDS             complement(13983..14240)
                     /locus_tag="PK733_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09737.1"
                     /translation="MTTTIQGNSYEWEGVRYEEGLCKCKKSLNQTANKVRVDFDFPEG
                     WVFASEIQVNEIDGTPGGDIPSDEVQNLALGQPIVNISYMG"
     gene            14351..14542
                     /locus_tag="PK733_03995"
     CDS             14351..14542
                     /locus_tag="PK733_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09738.1"
                     /translation="MYCIQSKLLGESIVERITTLEDATVSTEASPSEKIISLLHNGYY
                     RYIFSFSGIAIIYKGLGYI"
     gene            complement(14546..15238)
                     /gene="sfsA"
                     /locus_tag="PK733_04000"
     CDS             complement(14546..15238)
                     /gene="sfsA"
                     /locus_tag="PK733_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOJ09739.1"
                     /translation="MEYKNIRQAKFICRPNRFISHIEIDGKIEICHVKNTGRCKELLV
                     PGAVIFVQEFNIKTRKTKYDLISVYKGNRLINIDSQVPNKVFHEWLLNGNLVENITSI
                     TSIKPESKYRNSRFDFYIETVDNRRIFIEVKGVTLEENGVVLFPDAPTERGIKHITEL
                     CHCMNEGYEAYIVFVIQMKDVLYFTPNIKTHKAFGDALKAAKEQGAEIIALDTLVTES
                     SITAGDFVEVRL"
     gene            complement(15363..16724)
                     /locus_tag="PK733_04005"
     CDS             complement(15363..16724)
                     /locus_tag="PK733_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ09740.1"
                     /translation="MKNIISTLIKFVYAILLILSMLVGCTSNKTIIQKPVIKNSLEND
                     YLYSKYKDLVLYECYVDENGELVDFSCTYGEKILSQRSESLCESQIDYLKKIGYSQKQ
                     IDTFNVGDYDDIISGMLLNEDARINLRSDGLRENEYAKWTMLDFKNYYSKKLDAEGRC
                     INQIIYTDEMKKRIKEIKLSKNDIEKLTDLGYTIPEMIAMSKKEKDVLVISGAWRPFG
                     GSTADLLNMLPQNIALMTEKYHSIITKQQYGATVLNRPLEAYFLSSPNSDVKRKIVLT
                     FAMHGYEGDSKNDGVYLAENAYHIMLYYAINPDKLHDTQLIIIPMVNPDGVYSPDSTV
                     SYGRGQSLGIDMNRDFIISRFKAQESRYLRDLLLKEKPDLLIDFHGWYNGVYGDSTLA
                     RYFSDYMDLPQKDVMYGASQGYLIGYAKSIGIRSLLVEYNGIMDINCTNLILALNEII
                     GCE"
     gene            complement(16957..17724)
                     /locus_tag="PK733_04010"
     CDS             complement(16957..17724)
                     /locus_tag="PK733_04010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOJ09741.1"
                     /translation="MQNNKYFFEIDNNKITYITVKSKRKTIGITVDRNGEVKISAPLN
                     ISEKGIGEVVRKKAGWIIQKVNEARKRNSNLVSKEFIDGEKFLYLGENYSLKVVERNF
                     NKPEAIMQDDIIEVHILQGLSGESRKQVIKETLINWYKQRFAKVAKERIEKYSLQLKT
                     TPCKVVIKNQKTRWGSCSTKGNINLNWRLVMAPIGIIDYVVVHEMCHLRVMNHSKDFW
                     NLVESVLPNYTESREWLKVNGSRLSIEEYMNDFDVIV"
     gene            complement(18090..19649)
                     /locus_tag="PK733_04015"
     CDS             complement(18090..19649)
                     /locus_tag="PK733_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09742.1"
                     /translation="MKKNEIRKRFIVGLLICVFLIAAFAGCGKTTEDANKETKAGEQT
                     TGTKATEKEEPYEFTAFRSSWQGIDENNDLVVQELNKRLNIKIRILTAPYESWEEKYN
                     VYMASNTVPDVSCTNGTGWGNFNSWAKQGKYLDLTDLYQKHCPNIRKYLAGDVIEKVK
                     IGGKLYGVPKPSLDSGVLFYRKDWLDKVGLKLPETIDEFDAVVRAFTFNDPDNNGKDD
                     TYGMLNEDTLTQTKWLFMAFGTGANLYGGYDWFMKDDGTIESSIAQPGAKDAIKYLAG
                     LYKEGILDPEWMLTKSQGWYDKLVAGKGGIASQGITSYLTMEADMQKVDPKARLEPVP
                     YIKGKDGVIRRTTQNGYYMMNSISSKAEKPEKILELLDYMASDEGSRLIAYGIENVTY
                     EMKNGKPVYKEEEAKKYSVFGGHLLNRMMAQTGTGLPDDERGNAYKKLIEPYLMANIS
                     PIPPVELPSKKEVLSKQGADFVMKKLTEMVNNKIDIDANWDSFIKEWWETGGEQLTKE
                     INEAYKAAQGK"
     gene            complement(19693..20577)
                     /locus_tag="PK733_04020"
     CDS             complement(19693..20577)
                     /locus_tag="PK733_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09743.1"
                     /translation="MAYLKKKNNWFDVINCIILALMGIITLYPFIYVLTISLIPAEVY
                     LKGGLFLFPVRISFDAYRIVLSDKFVGSSFLSSVIITIGGTFCNLILTSMAAYALSKT
                     YLKGYRFFMGMFIFTMYFSGGLIPSYLLMSSLKLVDTYWAVILTGGISTYYMLVMRSF
                     YLNLPASMEEAALIDGASEVTVLLKIILPLSKPVIAALSLFFSVGHWNSYFTPMIYLN
                     NRNKWPLQIVLREILFETIASIKGSMDDMGGIKETLTVAAPLRMATVIVSIIPITVVY
                     PFLQKYFVKGVLIGAVKA"
     gene            complement(20624..21562)
                     /locus_tag="PK733_04025"
     CDS             complement(20624..21562)
                     /locus_tag="PK733_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09744.1"
                     /translation="MRISSYGVNNKKYSLLKYIWQKRFLYIILLVPLLYIVIFYYIPM
                     YGAIISFKEYVPGESIFKSKWIGFKNFEMIFQSRLFLQALRNTLIISTYRLVFGFPVP
                     IIAALLINEAKNKYYKKAVQTVIIFPNFVSWVIIGGIILSFLSVNDGLVNRILQYIGQ
                     EKISFLSESKYFRSILVLSGIWKEFGMKSVIFLAAISSIDISLYEAAIIDGANRWQRA
                     RYITMPEISNTTVILLILSLASILNAGLDQVLVLLNPMVSNVGETLDTYVYKVGLASG
                     EFSLSTAMGLFKSAVGFILIMSSNAITKKLKGESIF"
     gene            complement(21759..24176)
                     /locus_tag="PK733_04030"
     CDS             complement(21759..24176)
                     /locus_tag="PK733_04030"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ09745.1"
                     /translation="MNSKGYLRKIFNRRSINLYLKMFIVCIAISLFLTIIVGVFNHNY
                     IMRQFDQEIEKFEKGQVEKTANTIRMMFQEFEKIGQNYALHPDFNQLAYLKRDEFFDD
                     YYTVKKLMDILSSAINSSNYINDIFVYYENSNMILNFSAPLEEHIFYDMGWKEYYEQM
                     TENSILINTRKLDNSVNSRSSSYKNNVISFLTTIPYDGEEKLGTIVINVDYCIVSNIL
                     KNIINDSESVAFLASGDGTVLSSNNDEYLYSNIKEIINSQSSSINQEPANTNMIFKGR
                     KMVCYCSNVGINDWKLYIMIPSNIIFKKSLEIRLITILVLISILIIIAIASLLLSKKI
                     YKPIKAIIMLLKSEAGLKSIDINDTSYTNYTNYTNSFNDISGINNACDNSDISVIKNG
                     MNLLLKDKKSLEGLLDENRLLFREYFLINLITGKINDRKLIEKKAGYFNININYEYYQ
                     VAAIKNVSLYNADVDIEEYETKKLAMVNIVQKSFTEEKIDIICSQDIDDNILLLIKIH
                     DGANKKRLDSLILKCFETIKKRIEYYLGYEIIAGIGRIYDDILKIELSYKESLKAIKY
                     SFIAGDIVDICSINYSEDSESFYPLDMENKIISSIKLCDYEKVMLYVNRGVKEIIDNN
                     RDIDHILMSFFNLINFVNRCIFEFGLKPKNIMQQENYNNISINNFKNIDEFMGWLELV
                     FKKIVEHNIYMKEQAETDFSSKVKQYIEKNYMNNISLSSVSECFGYNSSYFSRLFKEN
                     LGVNFLDYMYQIRIEKAKILLATTNITIQEIAEQVGFTNRISFERCFKKFIEINPGEY
                     RTKMRYT"
     gene            complement(24448..26124)
                     /locus_tag="PK733_04035"
     CDS             complement(24448..26124)
                     /locus_tag="PK733_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOJ09746.1"
                     /translation="MILSKELLVEKRYLNFPVDTKGPKRLVKFIIDDCIIYESEIQMA
                     KGEPEFWVFLDVDKYKGRKLNVSVTNIEDKKDKADKAYKTNKENKGNKEDIQCDLDCI
                     IQEDSMIADDLYMEKYRPQVHFSSKRGWLNDPNGLIYYKGEYHLFYQHNPYGCRWGNM
                     HWGHAVSKDLVHWEELGDALYPDNLGTMYSGSGAVDWNNTSGFQICDEKVLVLIYTAA
                     GGFSEISKGQLFTQCIAYSNDKGRTWVKYSKNPVLRNLINENRDPKVIWNESMGKWVM
                     ALYMDKNDYALLTSPNLKEWTKISEITLPNCTECPDIFELRVDNDPNNRKWVFWGANT
                     TYIVGSLEDYGFKKESEYLKSHSGTNCYAAQTWNGLPIDDGRCIQIAWLKYNIPDMPF
                     NQSMTFPCELSLRTTDEGIRLFTNPVKEIEELYNKSHTFEMRKIDANSNSIIKMKTDL
                     LDVKVELNLEEAEVFSLNVCDINIEYIKQKSLLICLDQSVQLKAINNRLKLRIINDRT
                     SIEIFGNDGLVYMVMGAIRDEREGIIKLTSSKGDINVISMEVNELKSMWL"
     gene            complement(26206..26859)
                     /gene="pgmB"
                     /locus_tag="PK733_04040"
     CDS             complement(26206..26859)
                     /gene="pgmB"
                     /locus_tag="PK733_04040"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOJ09747.1"
                     /translation="MMIKLQAVIFDLDGVITGTANLHFAAWKKLTDEINVYFDRKINE
                     LLKGVDRMSSLEIILRNSEKQYTYEEKLSLTDRKNNYYRNLLETITADDILPGALNTL
                     KLLKNLKIKTGLASASKNAFLIIDKLGISKYFDYIADASKIKKGKPDPEIFLTTADNL
                     KVRSEECMGVEDSEAGIKAIKAACMYALGIGDSNILKDADYVIRSLEDFNINKLILL"
     gene            complement(26963..>27210)
                     /locus_tag="PK733_04045"
     CDS             complement(26963..>27210)
                     /locus_tag="PK733_04045"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09748.1"
                     /translation="SWMMDYQLRQFLSENSGIVRFQHRYLLYPRFATGKAVFSFVFNK
                     PFIEKLEDLPEDTSLLRAIKYHYLYGKYIYEQGGVFL"
BASE COUNT         8425 a         5299 c         4035 g         9451 t
ORIGIN      
        1 agaattacct ctgttcgagt actatgagta ctatgttttt ctatcacgag ttatctcata
       61 attttaactt ttggaaagct gacaatttca taaaaactta cagctatcat gctaattaca
      121 aaaaatatat aagaccttaa tataaatttt atcaggtgca tcctagttgt caatgtccaa
      181 atatgctaag ttattggtcc aatttttagc ttgcaggcag gtagcacata gaggggcagg
      241 tagaaagctg aaattgttct ggcaggataa caagtgggga cgattcttga acaaataggg
      301 gcggttctgg cagggtagaa atcagagaca agtggggaga caagtgggga cagtcctgct
      361 tattctttag cgaaacagga gaactgtccc catatccctg ttggatttaa atttccagca
      421 aggttttaat ccttaacatc aaataaccac ctgaaacaat tttcaggtgg ttatttgata
      481 aaattacttt atataaaata gcatataaat cagtttggat gcttcagctc tgttagcatt
      541 atctttaggt gcaaaggtgc catcaggtct tcctgtaaag aacttatacc tagaacccat
      601 tgcaactgca tctttagcat aatctgcaat tatttccttg tctttaaata catctatata
      661 agcagttttg tttgcaggca tctttttacc cattattcct gtaagaacat tactaagtat
      721 cttagccatt tcttctcttg ttattttgtc atttggagcg aatattcctc cgggtcttcc
      781 ctgtataata cctaggtttg cagcaactgt tacctctgga tagaaccagt ccttttcatt
      841 gacatcatta aagttattca ctgctttgct gtcaaacagc ttgaattccc ttactaccat
      901 tgctacgaat tcagctctgg taacatttct gtcaggagca aagaaattac cacctatacc
      961 catgataact cccttggaag ccataacttc tatataattt gctgcccaga aggtgcttgc
     1021 tacatcattg aatcttacat tatcaacttt aactgtatac atgctaaaat gttctgtttc
     1081 aaagtataca cgtttagtat cattattgta tacgccaatg atgttttcca gttctccttt
     1141 ttcatttata tagaatacag ttactttttc tgcatcttca ctgtccttga gtgtgtaagg
     1201 tatagaaacc ttaattagct tattaaattt cgctactctc tcgttgtcaa ctgtaaagga
     1261 caaatcatat ataggagcgt ctcctacact ctttttaact tcaccggata gagcttcatt
     1321 atctattttc tctgaattaa gtactataac actgtccttt ttcagttcaa tagcatcgga
     1381 tggaactgta aattctgcaa catcagttgt aacaactact cctgcaacac ccttttcatt
     1441 tgcagctata attagatttg caggtatttt caattcagca gaagctgtat tatcagcacc
     1501 gttaactgca ttaagataaa gcattggttt aatttcagct tccactcctg ctttttcaag
     1561 tgattctttc aactgttttg ctgttgctac tattaagtca agcttctttt cagtatattc
     1621 atcaacaagc ttgccatctt ctacttcacc aagtataatt tctccttcag cccttccttc
     1681 aactgcttga aaatcaaccc ttaaagtgcc cgcaatactt attacagttt caaccagttt
     1741 gcttaattcc tttgaaatcg aatcatgagc ttcataatta tttgacacag caagtttcat
     1801 taacaaaatt ccactattat caataagact cttggaaagc tctattgcta tttgaggctc
     1861 atcaataagg gaaacaagat tatttgcatt aattatcttc cggtatatat cttctgctaa
     1921 atcggtaagg gatatatcaa ttacagaagc tgtattccct tcttttattt cattaataat
     1981 gttcttaacc tgaactatga tctcattctc ttctcctgat tcctgaggtt cttcaacgtc
     2041 atcaggagtt gtttcctctg atggttttgc aggctcagcc ggttcatttg gtactgttgg
     2101 gccttgaggt tcttccggct cgtatggttc ttcaggatct ttgggatcag ttggttcttc
     2161 aagatccggt tcacccggct tttcagaagt aaattggtaa gggcctatat caacagcttt
     2221 atctgacggt cttgcattgc catcaaaatc tacagaaggt gcaccaagtt ttgtaccagt
     2281 cgcaatacaa ggactagatt ctattaagtg atagtcacca cttccatcag ctttaaagtc
     2341 aacaaactta ggatcaactg ttaatgtatt tatgtctgct ttaccatctt gcaattcaaa
     2401 tgcattacca gtattctcat atactaaatt gttctcatat ttattgttcg gacctgtagc
     2461 acctgcttcg ataataccaa cattaccatt atgtattaca atattgtttg ttacaataaa
     2521 gttgtctgct ataataccaa ctggagtctc acctgcacca accttaatcc cacctgttcc
     2581 atttttaaaa acaaggttat tagaaatagt gcaatttctt gctgcattcc atgtatgaat
     2641 cccaaacccg ctattgctat gggaaatatt attaatcact ttcatattat ttccggcaat
     2701 atagatacca tgtatattgc ctccacttcc gtcttttgga ccaatatcgt gtattaaatt
     2761 tccaattata tctacactat gaattttata cgcctcacta tcatacgtga cgatggcaga
     2821 accccaagga ccaccattag gtgctatgcc atatatatgg ttgctaataa ttcttacatt
     2881 tgattctctc acatttatac catataaaac atcgttccca gttatctcaa acccttcaat
     2941 ttcaacatag ccttcttcaa ctgtcaaagg tagtgagccc ctgctgacta cattgatttt
     3001 tgcgccccat ttttcttccg aaacaagcct tatccatgct tcctcagtcc cgcctttatt
     3061 aatcttaatg ggatcagtat agatacctgg aagtacatga actgttgcgc caggagtagc
     3121 agcagttgca gctttgttta tagttgcaaa cgggtattcc ttagttccag gattatcatc
     3181 atttccttgc ggtgatacat atatatgcag caatcgttct gcttcttcaa atacccattc
     3241 tccattgcta agttctgcac tcattactct ataaccatct ttaaatgtaa actctgtcac
     3301 attttccggc accatgaaat taccatcagc aaaaaacact ccattagagc tttctgtgat
     3361 atctccttct gcttcataag gaacttttac tcttataggc agttgaccgg taaattgaaa
     3421 aggacctata tcgatagctt tgtctgctag tctttcattg ccatcgtaat ctatagaagg
     3481 tgcgccaaat tttgtgccat tacctataca aggactaata gctgttaaat gataatctcc
     3541 gcttccatct gatttaaaat caactaattg cggatcaaga attagtgtat tatggtctat
     3601 tttcccatca tgcagtctaa acgcctttcc aacatttcca tagactacat tattctcata
     3661 tttgttattt ggtcctgttt tacctaattc aataacgcca tcgttattat tatagattat
     3721 aatattattt gtaacaacaa aattgtctgc tatgattgtg ctatcgctag aaccaatttc
     3781 gactccacct tttctgttcc caaaaataag gttgttggat attatagtat ttgttgcttt
     3841 atgccatgca tgaatccccc aaccggcatt gctatacgta atattgttta atatcttaac
     3901 gccggtgcct acggtataaa ttccatggat aagattgctc tcttcacctt cagggccaat
     3961 gttatgaacc agatttccta ttacctctac actatcatga gctactatag ctgcgccacc
     4021 taaacgatct tctttaggcg ctaggtcata aatatgattc ccgataattc taacattaaa
     4081 tccatctcca ttaataccat ataaacaatc tcccgttatt tcaaaccctt caatatcaat
     4141 atagtcacca tagaataaca tagctgtcgc cttatctccc tgacgggcta cgttaatttt
     4201 agcgccccac ctttcttctg atacaaacct tactcttgcg tcagcagtac cacttttatt
     4261 tataattata ggttcggtat agattccagg tagaacatga actgttgtgc cgggggtagc
     4321 agcttttgca gctttgttta ttgttgcaaa aggctgatca atagtcccta gattatcatc
     4381 gttgccttcg ggagaaacat atatatgttc tattttctct gcttcactaa ttgtccattc
     4441 atcattagcc agaactgctt tcatatattt gtcgccatct ctaaaagcaa actctgatat
     4501 accttctggg actataaatt taccattaac aaaaataact tcatcagaga tatctgtaac
     4561 ttcgccttct gcattatagt gaacttttac taataaaacc ggttgtatct tatctgacat
     4621 ataaatagtc caacgtgaaa gatcaacccc ttgttcaaaa tcgtcttcaa ttatcaattg
     4681 ttcatctgcc cataagctta cattatccat atatgtcaag ccctgttctc caagaggact
     4741 cacatcattg gcagtaaatg gatttgggta agtaggtgtt ccattcccga accctttacc
     4801 tataatcaag tgagatggag gttctgaaga tggtgcatta agtgtagtta acagaccaag
     4861 ctccgttcct ttttcccctc cccacatttc aagctttcca ttttccaaaa ccattcttac
     4921 agtataccat tttccaagcg gggtattccc gaaacgtcct gatgcaccat atataataaa
     4981 ttgtttttta tatgaatcaa tccctattga tacccatttg ctttcactcc agtagagttt
     5041 taatgaaggt gtccagctag ctccaggatt tgttattggc aaaatatccg cttctgctac
     5101 tattttccca ggttcagaat tattccccca gtttagctct gtaacaagta aattgtgagc
     5161 attctcaggg cctaaaaaca ttaccaggcc gtcatctaca tgagaacaat cgtcaggttc
     5221 atcttgtttt acttctttaa ttacccattc atcactatta aatactacct tcatatattt
     5281 gttgccatct ttaaatgtaa actctgctac actctctggt acaataaatt tcctgttaac
     5341 aaaaataact tcatcagaga tatctgtaat ttcaccttct gcatcatagt aaacttcaat
     5401 taaagcagca ggttgtatct tatctgacat ataaatagtc caacgtgaaa gatcaacccc
     5461 ttgcccaaaa tcatcttcaa ttattaattg atcatctgcc cataatctta cattatccat
     5521 atatatcaag ccctgctctc caagagggct tgcatcattg gcagtaaatg ggtttggata
     5581 agtaggtgtt ccatttccga acccttttcc aataatcaaa tgagatgggg cttctgagac
     5641 aggtgcatta aatgtagtta acagattaag ctccgttcct ttttctcctc cccacatttc
     5701 aaccttccca ttttctaaaa ccattcttac tgtataccat tttccaaggg gagcattccc
     5761 aaaacgccct gcattatatg ttagaaattt accgtcagtg cttatcccta tcgataccca
     5821 tttactttca ctccaataga atctcagtga aggtgtccaa ctatcactag gattttttat
     5881 tggtaaaata tctgcctctg ctactatttt tccaggttca gaatcatctc cccaatttag
     5941 ttctgtaaca agtaaattat gagcattctt aggtccatgg aacattatca taccatcatc
     6001 tgcgtgagaa tagctgtcag catgaacttt tgatacaggt aaaattacac caatcagcat
     6061 gtataaaaca aacataacta tagtaatgtt tgcatacaat tttctttttc tattgctata
     6121 aaaaaacatc ttttacatac ccccattcat catgtaatta taataaactg catcagagga
     6181 cttactctaa ttatagtgtt tacctcctga atagtatttt tacagttcca tcatcctcta
     6241 tttcacctga tatctctgta ttcattaatt gtaaatttac aataatatcc cataccggtc
     6301 cagcattcgc cagaccgata ttagatatat agacatagat atgggcatgg tgttaattat
     6361 ttaactgcat gaactaatgt ttatctttat aggttctaat ttatttacca tatttctctt
     6421 catatgcttt ctggtaaacc ggaagaattt tttcaaggcc attcttgttt atagtctcca
     6481 cgtatgtttt ccaggtagcg ttatctgttg gatcaagttg actcatgata aacttaagcg
     6541 tatattcagc catcttatca tttacaacgg ttccaaaatc agcaattgta gccttttcag
     6601 catcgttgaa tactaatgag taaggtttga aataatcata gttattcttt cttacaagat
     6661 caccaattac agtggatggt ccacctccct gatagtagaa gaaacgagga tagtttatga
     6721 ttctatattc aagattgtac ttaagcaaag tgtctgtacc tttatcagga ttgagcggag
     6781 taattatatt atccatatat tcaatctcat tatttgaatt atacttaaag gttacacctt
     6841 cttctcccat ttgagtcttt atcataccct cttcagttgc cagccagtca aaaactttaa
     6901 ttaagcgatc gattctttcc tgggaatttc tgcttgagat tcttgctgaa ctagctccaa
     6961 cagctgaata tctggaaacc attaattttt tgtcaggata agcattaata aatttggggc
     7021 tggatagcca gttggcttta ggatcgactt cagtgcaaag attgttagcc cagccttcac
     7081 gtgcaaaata ctcaagtaga acatgaactt tgccttgtcc aacatgctca ctccactgtt
     7141 gagcagattg agtagagaac tccttggata tcaaatcttc cttataaagt ttgttgagat
     7201 aaattaacat gtcacgatat ttatcagtaa tcggttcata tacaaactta ttgtaatcac
     7261 catcatactg gaaaattgag ctagttctaa atgcatcttc aaaacgccgg gttaaccccc
     7321 acaagccatt tctgttggat ataggtatag aatctggata tttattttta atagctttaa
     7381 gataggaata tagctcctgt gtagtttcgg gagcctttac gccaagctct tcgattgtac
     7441 tcattctgaa cagccagccc tcacctacca actgaacatt ccccatgggt acacgaagag
     7501 agtaatactt gccgtctgga gctgttgcca gttccaggta ttcctgccaa aactcatcag
     7561 gccattgcgc cctatagttt ggaagtttat caaagtaatc atcaaaggct atgaaataac
     7621 catctacgcc atacttatca aagtcaccaa aaaggttaaa atggacatct ggatcctcat
     7681 tacctgccat tctaagcttc atagtttccg ccaaagagct ttgtgctatg tagtcgattt
     7741 ttagtgtgat attaaacttt tcgcggagtg ccttggcgaa caaatcagta ttaggattgc
     7801 tggcggcctc ctgtgaagtg gttacagttg taaaagtcat gggagaaaac tgttcaagtt
     7861 ctgatacagt attttctgca gtatttgatg tagtcttttc cttattgctt gaatccgggc
     7921 ttctttttgt tccggcacag ccagcagtta tacctataat cattacaact gccaaaaata
     7981 ttgctatagt ttttcttttc ataattatac ttcctttctc tttttatatt tttgatttat
     8041 ttaccaacgg cttttaccgt agtatccggt tgaatggctt tagcctttta aggacccaat
     8101 cataacccct ttgacaaaat atttctgtac aaatggataa attatgagca taggaatcat
     8161 tgaaacaaat agggtagcat acttaagagt atcagcattt accatctttt gagcatcatc
     8221 tttcataaca ttcatgcttt tacctgcatt taaattactg gcaacaccac ctacatccat
     8281 catatcctct aatttatttt ctataatcag ttcccttaaa attaaaggta aagggtactt
     8341 cctactggat tttaaataga tcatgcccgt aaaccaatca ttccatttac ctacagcgta
     8401 aaataagcca atagttgcaa ttgaggcagt agaaagcggg ataataattt tgataagtat
     8461 tatcagatca tttgcaccat caagatatgc tgattcctca agttctcgtg ggagactttt
     8521 aaaaaatgat atcataatca tagtgttata tgtacttata gcaccaggca gtagtaatgc
     8581 ccatacagaa tcatataatc caagtctctt aataaggagg aagctgggta tgagaccacc
     8641 agaaaagtac atagtaataa caaggaaaac cgtatagaat ctccttaagg gcaaatcctt
     8701 ttttgaaagc gggtatgcca ataaaattgt cataacaagg ctgatgatag ttccaacaac
     8761 tgtatagaat atagaatttc taaatgaatt tggaataata tcattcttaa atatcaattc
     8821 atatgcttta aaagtaagtt ctttcgggta aaacgttaca agatttttct ctacagccac
     8881 agaactagac aatgatgtag cagcaatatt taaaacaggg tatagtgtta tgaccacaac
     8941 aaaaataagg ataatgtgaa ttataatatc aagtattttt gaccctgttg tattgtcttt
     9001 aaccattatt actttccttc ctttctagat ttattctagc gttttttaat gttgatcaat
     9061 tataaaaagc tcagtatagc tgcttgttaa ttagtataac tgcttattca attattacca
     9121 aagactaaag tcagcaaact tacgtgccat cctattagca atcgtcaata gtattacatt
     9181 aactatagaa ttcataagtc ctacagcagt agagtaacta tattgagcat caaacagacc
     9241 taccctgtaa acaaaggttg aaacaacatc cgcaacttca tatgtaatag gagaatacat
     9301 aagaataatc ttttcagagc ctacagaaag cattgaccca agtccaagga taaatagaat
     9361 aactatggtt ggctttatac caggaagggt aatgtgccat atttttctaa aacgaccacc
     9421 tccatcaatt gaaatagctt catatagctc ttggtctata cttgatagtg ctgcaagata
     9481 tataatagca ttccaccctg tactctgcca tattccagat ataatataaa ttggtctaaa
     9541 ccagcactgc tccgacataa agtatatagg ttctatttca aaccatgatg tgagcaattt
     9601 gttgataaag ccactggatg gagacagcat cattacaatc atacctacaa ttgctactgt
     9661 agaaatgaag tgtggaaggt aactgacggt ttgcacaaac tttttaaatt taatgttgcg
     9721 tacttcattt aataataatg caaatacaat tggaatagga aatgagaaaa taagggaata
     9781 gcaacttaat aatacagtat tgcgcataag cttccaaaaa tatggatgcg ttatgaaacg
     9841 ttcaaagtgc ttgaacccga caaaagggct atttaatatg ccaattcgca tgttaaaatc
     9901 tttaaatgcg attgttactc catacattgg cccataacaa aagattatat accaaataat
     9961 tgcgggaata agcatcaatt gtagatattt agttttttta atttctttcc ataatgtatt
    10021 ccatcggaaa tccactataa cttttttgtg ttttacagca tccacagaaa caaacacctc
    10081 ttttatagaa ttaaagcatt tcattttttt aaatgctaga ttcaatctac aataagagtt
    10141 ttgcattgta aagacacaat ttcaaaactg agaaacaata tgatgaaaaa tgatgataca
    10201 tttgccacac taaggaacaa taggcccaaa aggcgcataa taagcgaaaa aatcaaaggt
    10261 ttctatttac ttctatttga aaccgcgata taattttgta caaataaatg tatttatagg
    10321 aggggctttt tatggaaaaa aataataaaa agcattctaa aatacttaag cggtttttgt
    10381 tttcttattc tgttattatt gtactgcctt tgctggttgg attaatatcc tataaggaat
    10441 ctgctacaat aatatcgaac gatacgaaaa atacccatct atcaatgctg cgccagagta
    10501 tggagataat ggacaggatt acgggggaaa cagatagaat tgcttgccag atggcagtaa
    10561 atccacaggt tattaaattt tgttatgcat atgattttaa tgataaatct atctactata
    10621 atatgtggga aactctatcc tatataaaac catattctgc ttatgagaat gccggttcag
    10681 aatataatat ttactttaac aaaagtgata tggtttttaa tcaaaatggt gcattaactt
    10741 ttgatgaata ttataaacac tttggttatc agaatataga tatagatgaa ttcaggcaga
    10801 gatttctaaa tatcttcaac aaaggcacat ttatatcttc agaaactgtt aaatctacag
    10861 aaataactac aaccagtaaa aagatatcca attataatgt aattacatat tttaaaacaa
    10921 tgccgctgat gaataccggc atactgaatg cagttttatt tgtgcgaata aaggaagata
    10981 gcattctgaa atttttaaga ggactggatg tttcagatga tggatgcgtt tacattctag
    11041 acgaaaattc agaaattatt accggaatac ctgccggttt agggaaaagc gtcccataca
    11101 acattgactt tatcggttcc gagggctttg tagagaaaaa tatcaacgga aagactatgt
    11161 atatagtcta tacagtttcc tcatataacg gctggaagta tgtatcggca gttcctgctc
    11221 atattgtaat ggctaaggtc aattccatac gtaatcttat attgggaata actgttgtat
    11281 cactgataat aggtattatt ctattaatat tattagctta tgccaattcc aaaccaatta
    11341 ttgagttaaa gaaaactctg ctggatttct ttaactctga agatgacaac aaaaaagacg
    11401 aatttgattt tttaaaatcc agtatatcag atttaatcca tcaaagctct gctatgaaat
    11461 ctgacataca aaagcagcgc tcaaccctcc aaaaatcggt tatcgagcgt ttgctcctgg
    11521 gcagaaacat cccctccagt attctggatt gggcttctca ggctggtgtt gaattaggtg
    11581 cacaagcata tacatgcgtt ctcataggtt tgaagaatgt acagatagaa aacgatcagt
    11641 tgcaggaatt caatattggg caaataatac ataatcaaac cgggcaaaaa ggttatttaa
    11701 tagatattaa ccctaacacc ctttgtttgc ttctatgtct caacactaat gaggagcaaa
    11761 aatgcaagca gcatacggaa agagttatat catggattag taatcagatc caggagaacc
    11821 ttaatatcat acctaccttc acaatcggct ccatatatta tgatttgagc gatatttacc
    11881 acgcttataa cgaagctaaa caggctcttg attttatgcc tatcagagaa ccaaatacaa
    11941 tattatggta ttctagtctt gaaatggatg caaaaagcag ctattattat cctattgaag
    12001 ttgaacagcg tctgattcat cttgtaaagt acggctacaa ggatgattta tctaatctgc
    12061 tttctactat ccttgaaaaa aacctggaaa atccggcact tttgcataaa tcacagatgc
    12121 ttctgcgcca tgaaattaac ggcactattt ataaattaaa cataggcaat ccagaagatg
    12181 tggttgcaga gcttgacaat tgcgaatctt tgaatgatgt atttaaatgc atgcaaacag
    12241 ttctcaataa tatgtgcgat gatgtaaacg agcagaaaaa caatcgcaat attaagttgg
    12301 ttgatgaaat tattgcgtac atggaatcga atttcagtga ttcaggtctt tgtcttagtc
    12361 aagtagcaac agaatttaat cttagtgagg gttacatatc acagctgttt aaggagcata
    12421 cgggtgagta ctttgcagca tatcttgaaa atatcagact tgaacatgca tgccaattac
    12481 ttgaacggag ctcttatact ataaacgaag tagctttgcg ctcaggctat aattctcccc
    12541 agtcattccg ccgtgccttt aaaagagtaa atggctttaa tccttcggat tacaaggttt
    12601 aaggcaatca cgtaaggcaa gtgagcgctt atagtcttac ccctacaaaa tctccggcag
    12661 ttatggggct ttctttaacc agtgtatcaa gggcaataat tttagcgtct tgcaacatcc
    12721 cgtgttttta acatgccata cttcttcttt gccatcaatt tctatatgtg caacaaatct
    12781 attcgggcgg tatataagct ttgtttgctt tatgttttca tattccataa taaacagtta
    12841 ccttataaat ttcactttct taaatctgat ctactgatta ttctgcttat catcctcctc
    12901 tggttttaat catacatcat ccggggtcaa atgacctaat gctattataa taccggaacc
    12961 tcttcctctt gcgattgtgt tgtatattga ttgaacctgc tgctggtact taatactgtc
    13021 atccttatgc caactaccga tttgcctgct tttttagcta tttcagtaac aggcatatta
    13081 ttattttgtg tagaaagaac tgtcgtcccc aagctgcttg agccactact caaggcaggt
    13141 gtgacatttg aatctgaaac taaccctaca ttctttatac tgctgttatt tactgcttca
    13201 tttatatatt acccatattt ttagacgcta ttgtgtagaa attatgaatt atttttgaat
    13261 tttaatttgg aatataatac tttaaaactg ggaaaaataa tattaaaatc acaaaatcat
    13321 aacaataaat tagtaagggt gatgtatatg gaaataagag agaatattat ggttcccttg
    13381 gggtatggaa aatttgtgcg ttctgataaa attgtcagtc tagagcctat agaggacgaa
    13441 agaggaccgg gaagacgtac aacagtatat atagaggatg taaaatcacc attaatcgct
    13501 tcaagaacag aaaactcgat tcttgccaat atggtagagg ttccaaaaga ggaattggaa
    13561 gctgcagctg cactggaatt attgaaagac ataagagatg atatcttgca tataggtccc
    13621 atgctcagga aaagtataaa aaaagaagct agctttgata ttgataaaat cgaaaaaaga
    13681 ataaatgaaa tactcaagca tgaaattgag tatgacaact ccttttaaag caaatactgt
    13741 tactagtaat aaactgttaa tagtacaatg tcaggataac caagtatctg gagttcatct
    13801 gcaaatacaa atacaccaag gtcaaaatca attttcacat gggtagcttt ataatttagg
    13861 gcatctatct tatacttata atttttggta gtcctaagtc aacctgtatt cggtctgttc
    13921 ttcagtagta accttttggc cattctggtc aagtcctgta tcagctccaa gtccggatcc
    13981 ttttatccca tataggagat attcacgata ggctgcccaa gagccaaatt ctgaacttca
    14041 tcagaaggta tgtctccacc tggtgttcca tctatttcat ttacctggat ttccgatgca
    14101 aatacccatc cttcaggaaa atcaaagtct actctaactt tgtttgcagt ttggttaaga
    14161 ctttttttgc atttgcataa cccttcttcg tagcgtacac cttcccactc ataagagttg
    14221 ccttgtattg ttgtagtcat acattcatct cccattttaa aaaggagcgt tctgcatggc
    14281 ctatcgtaaa gtgaatatga aaaggggaca gttcttctgt taaaggggac aagggggaga
    14341 acagttcttt ttgtattgta tacagagcaa gcttttaggt gagtctattg tggagagaat
    14401 tacaactcta gaagatgcaa cagtgtcaac agaagcctct ccgagtgaaa aaattattag
    14461 tcttctacac aatggatatt accggtacat cttttctttt tcaggtatag caatcattta
    14521 taaaggcttg ggttatatct agcgcttata gtcttacctc tacaaaatct ccggcagtta
    14581 tggagctttc tgtgaccagt gtatcaaggg caataatttc cgctccttgt tcttttgccg
    14641 cctttaaagc atcaccaaaa gctttatgag ttttgatgtt gggagtgaaa tatagtacat
    14701 ccttcatttg aataacaaaa actatataag cttcataacc ttcattcatg caatggcaaa
    14761 gctcagttat gtgctttatc cctcgttcgg ttggcgcatc aggaaataaa acaacaccat
    14821 tttcctccaa ggtaacacct ttaacctcaa taaatattct gcggttatca acagtttcaa
    14881 tataaaaatc aaagcgggaa tttctatatt tactttcagg ttttatcgaa gtgatcgaag
    14941 tgatattttc aaccaagtta ccgttcagaa gccattcatg gaacactttg ttaggcacct
    15001 ggctatctat atttattagt ctgttacctt tgtaaactga tattagatca tattttgtct
    15061 tacgtgtttt aatattaaac tcctgcacaa atattacggc accgggaact aataattcct
    15121 tgcaacgtcc tgtgttttta acatgacata tttctatttt tccatcaatt tctatatgtg
    15181 aaataaatct attagggcgg catataaatt ttgcttgtct tatatttttg tattccataa
    15241 tctctcccat agaattgcgt aaattaattt gcgtatctgt aattatactc atagctatca
    15301 tcatttaatt atatagaatt ttataaaata aacaataccc cacacatgct ggggtgcacc
    15361 ggtcattcac aaccgattat ttcatttaaa gctaatatta agtttgtaca gttaatatcc
    15421 ataatgccat tgtattcaac tagcaatgag cggattccaa tagattttgc ataaccaatc
    15481 aaatatcctt gtgatgctcc atacattaca tctttttgcg gcaggtccat ataatcacta
    15541 aaatacctgg caagggttga atcaccatag accccattat accagccatg aaaatcaatc
    15601 aagagatcag gtttttcttt taacaaaaga tctctaagat acctactctc ctgcgcctta
    15661 aatctgctta tgataaaatc cctgttcata tcaataccta gagactgacc tcttccatag
    15721 gaaacagtgg aatccggtga atatacacca tcaggattta ccattgggat tataataagt
    15781 tgagtatcat gtagcttgtc ggggttaatg gcataatata acataatatg atacgcgttt
    15841 tcagctaaat atactccatc attcttacta tctccttcat atccatgcat agcaaaagtc
    15901 aagacaattt ttctttttac atcagagttg ggggatgata ggaaataagc ttccaaggga
    15961 cggttcaaaa cagtagctcc atattgttgc tttgttataa tgctatggta tttttcagtc
    16021 atcagggcaa tattttgagg gagcatatta agcaggtccg ccgttgagcc tccaaaaggc
    16081 ctccacgcac cggatattac cagtacatcc ttttcctttt tagacatagc aatcatttct
    16141 ggaattgtat accctagatc agttaatttt tcaatgtcat ttttactgag ttttatttcc
    16201 tttattcttt tcttcatttc atctgtataa ataatttgat taatgcatct accttcagca
    16261 tccagttttt tagaataata atttttaaag tccaacatag tccactttgc atattcattt
    16321 tctctaaggc catctgaacg gagatttatt cgggcatctt cattcaaaag cattccagat
    16381 attatgtcat cataatctcc aacattgaaa gtatctattt gtttttgaga gtatcctatt
    16441 tttttcaaat aatcaatttg actttcacat agtgattcac tccgctgtga tagtattttt
    16501 tccccatatg tgcaggaaaa gtcaacaagt tctccgtttt catccacata gcactcgtat
    16561 agtactaagt ccttatactt gctgtacaag taatcatttt ccagactatt ttttattacc
    16621 ggtttttgta ttatagtttt atttgaagta caaccaacta gcatcgacag aattaatagt
    16681 atggcatata caaatttaat tagtgtactt attatgtttt tcattgaaca ccatctttac
    16741 atgctgtaaa taatcttctt cattctaata atctactcta tattactttg atttatccgt
    16801 ggataattat tcctaaattt tttttacaaa attcttcctc atataacaac ccaatatctc
    16861 acacgggcta tttctgctcc ttttgcttta aatgttgaaa ttttttaatc gagaatataa
    16921 ttatagttaa attctacaaa gcttaaaaat acaaaatcaa actataacat caaaatcatt
    16981 catatactct tcaatgctta atctgctccc attaactttc agccactctc tgctctcagt
    17041 atagttcgga agtacagact ctacaagatt ccaaaaatct tttgaatggt tcataaccct
    17101 cagatggcac atttcgtgta caactacata atcaattata cctataggag ccattaccaa
    17161 tctccagttt aagttaatat ttccctttgt actgcagctc ccccatctgg ttttctgatt
    17221 tttaattacc actttgcagg gagtcgtttt gagttgtaaa gaatactttt cgatcctttc
    17281 tttggcaacc tttgcaaaac gttgcttata ccagtttatt aatgtttctt ttatcacctg
    17341 ctttctgctt tcacctgaca atccctgtaa tatatgtact tctataatgt catcctgcat
    17401 gattgcttca ggcttattaa aattccgctc caccactttt aaggaataat tttctccaag
    17461 gtacaggaat ttctccccat caataaattc tttggatacg agatttgaat tcctttttct
    17521 tgcttcattt actttttgta taatccaacc tgcctttttc cgtaccactt ccccaatccc
    17581 tttttcgctt atatttaaag gagcagatat cttaacttca ccatttctat caacagttat
    17641 ccctattgtt ttccgcttgg atttaactgt aatataagta attttattgt tgtcaatttc
    17701 aaaaaaatat ttattgtttt gcatattaac ctcattaaag tctttcacac tgcatccccg
    17761 aacactttat tttacgtatc aggtaaatag tcaatgcttc aaaattataa ttatttcaat
    17821 attatcacga taaagtataa aaataaagtg tttaaaaagg ttagaggcgc atttatcaaa
    17881 tctgaagaaa tatttaccag aggctatcgt taaggtactt atagccttca tttatgcccc
    17941 tcaccaattt caggtgaagg acttttcatg ataaaagaat agagacattg gtttcatgcc
    18001 tctattcctt tgcatttaca ttctcctatc ggattcatta tatctgtttc actgcatctg
    18061 attttttata tcggattcat tatatagttc tacttgcctt gtgcagcttt atatgcttcg
    18121 tttatttcct ttgtcaactg ttctccgccg gtttcccacc attcctttat aaagctgtcc
    18181 cagtttgcat caatatcaat tttgttgtta accatctcgg taagtttttt cattacaaag
    18241 tctgctccct gtttagataa tacttctttt tttgaaggaa gctcaacagg aggaatcggg
    18301 cttatgtttg ccataagata tggttctatt aatttcttat atgcatttcc cctttcgtca
    18361 tcaggaagac cggttccggt ttgtgccatc attctgttca gtaaatgtcc accaaacaca
    18421 ctatattttt tagcttcctc ttctttatat acaggtttac cattcttcat ttcataagta
    18481 acattttcaa taccatatgc aataagcctg ctgccttcgt ccgatgccat ataatccaat
    18541 agctccagga ttttttcagg tttttcggct ttacttgaga ttgaattcat catataatat
    18601 ccattttgag ttgttcgcct aatgactcca tctttgcctt taatgtatgg aacaggttca
    18661 agccttgcct tcggatctac tttttgcata tctgcttcca ttgtaagata tgaagtaata
    18721 ccttgtgatg caatacctcc cttacctgcc acaagcttat cgtaccaacc ctgagacttg
    18781 gttagcatcc attcgggatc aagtattcct tccttgtaaa gtcccgccag gtattttata
    18841 gcatcttttg caccaggttg tgcaatagat gattctatag taccatcatc cttcatgaac
    18901 caatcatatc cgccatatag gtttgctccg gttccgaaag ccataaacaa ccattttgtt
    18961 tgtgttaatg tatcttcgtt aagcatcccg tatgtgtcat cttttccatt gttatcagga
    19021 tcattaaaag taaatgctct cactactgca tcaaattcgt caatagtttc aggaagtttt
    19081 agacctacct tatccagcca gtcttttctg tagaatagta ctccagaatc aaggctaggt
    19141 tttggcactc cgtatagttt cccaccaatc ttaacttttt caataacatc accagctaaa
    19201 tatttgcgaa tattagggca atgtttctga taaagatcag taagatcaag atatttacct
    19261 tgttttgccc aactgttgaa atttccccaa cctgtcccat tagtacaaga aacatcaggc
    19321 acagtattag atgccatata cacattatac ttttcttccc aactttcata aggtgcagtc
    19381 aatatcctta ttttgatatt aagccttttg ttcagttcct gtactactag atcattattt
    19441 tcatcaattc cctgccagga agacctgaat gcagtaaatt catagggttc ttccttctct
    19501 gttgctttag tgcctgtggt ttgttcgcca gcttttgttt ctttattagc atcttccgta
    19561 gtttttccac atccggcaaa tgctgcaatc aggaaaacac aaatcagcaa accaacaata
    19621 aaccttttcc ttatttcatt ttttttcata actatatcct cctatttaat tttttatgta
    19681 aacctgttgt attcaggctt ttaccgcacc tatcaaaaca ccttttacaa aatatttttg
    19741 taaaaaggga tatactacag tgattggtat aatcgaaaca attactgtag ccattcttaa
    19801 aggagcagca acagttaatg tttctttaat ccctcccata tcgtccatgc tgcccttgat
    19861 tgaagctatg gtctcaaaaa gtatttctct taaaacaatc tgcaatggcc acttattccg
    19921 gttattcaaa taaatcatag gtgtaaagta gctattccag tgaccaactg aaaaaaacag
    19981 gcttaaagca gctataacag gttttgataa tggaagaatt atctttaata atacggtaac
    20041 ttccgatgca ccatctatta atgcagcttc ttccatactt gcaggaagat tcaggtaaaa
    20101 gctcctcata acaagcatgt aatatgtact tatacctcct gttaaaatta ccgcccaata
    20161 tgtatcaaca agtttcaaac tgctcattag cagatatgaa ggtatgaggc cgccggaaaa
    20221 atacatggta aaaataaaca tacccatgaa aaacctatag cccttaaggt aagttttaga
    20281 gagtgcatat gccgccatgc ttgtaagtat taaattacag aacgtacctc caattgttat
    20341 aataactgat gaaagaaagg aggagccaac aaatttatca ctgagaacta ttctataggc
    20401 atcaaatgaa attctaacag gaaacaggaa gagtccccct ttcaggtata cttccgcagg
    20461 tatcagagat attgttaaaa cgtatataaa agggtacaat gtgataattc ccataagagc
    20521 tagaattatg cagtttataa catcaaacca attgtttttt ttctttaaat atgccatttg
    20581 aattcttcca cctttattaa aaaattaaaa aataattgtt attttagaat atactttcac
    20641 ctttaagttt tttggttatt gcattagagc tcataataag tataaagccc actgcagatt
    20701 taaaaagccc catggctgta gacaaactga attcgccgct tgcaagtccc actttatata
    20761 catatgtatc aagagtttct cctacatttg ataccattgg gtttaggagc accaatacct
    20821 gatcaagacc tgcatttaaa atgcttgcta gtgaaagtat aagtaatatt accgttgtgt
    20881 ttgatatttc gggcatagtg atgtacctgg ctctttgcca tctgtttgct ccatcaataa
    20941 ttgctgcttc atataggcta atatctatac tgctaatagc tgcaagaaat attacagatt
    21001 tcattccaaa ttccttccat atccccgata aaaccaatat actcctgaaa tacttagatt
    21061 cagacaaaaa tgatattttc tcctgcccaa tgtattgaag tatcctatta accagtccat
    21121 catttacgga cagaaaactt aatataattc ctccaataat aacccatgaa acaaaatttg
    21181 gaaaaataat aactgtctgt actgcctttt tataatattt gttttttgcc tcattaataa
    21241 gaagagctgc aattataggt acagggaaac caaaaaccag tctatatgta ctaataatta
    21301 atgtgtttct taaagcttga aggaataatc ttgactggaa tatcatttcg aagtttttaa
    21361 aacctatcca tttacttttg aaaatacttt ctcccggtac atattcttta aaagaaatta
    21421 ttgctccata cattggtata taataaaaaa taacaatata aagtaatggt accagtaata
    21481 ttatataaag aaaccttttt tgccatatat attttaaaag actgtacttt ttattattta
    21541 caccatagct gcttattctc attctcacag aatttgtcac tcctttttag tatgtattgg
    21601 aaagtcttaa gattgattcc agctttatgc ttaatgctga ttctagtata tagctctgtt
    21661 agtggagtga caaggttcag tattttacat tctattcaga aatattgttt tctgaaaaat
    21721 tggtaaaaaa gatatacata tgtaaatatc tttaaaaact atgtatatct cattttcgtc
    21781 ctgtattcgc cgggatttat ttctatgaat tttttgaaac atctttcaaa gcttatcctg
    21841 ttagtaaagc cgacctgctc agcaatctcc tgaatagtga tatttgtagt tgcaagcaat
    21901 atttttgctt tttcaattcg tatttgatac atatagtcta agaagtttac acccagattc
    21961 tctttaaaaa gtctcgaaaa gtacgaagag ttgtagccaa aacactccga tactgaagat
    22021 aacgaaatat tgttcatgta atttttttct atgtactgct ttacttttga ggaaaaatct
    22081 gtttcagcct gctctttcat ataaatatta tgttcaacaa ttttcttgaa tactaattcc
    22141 agccagccca taaactcatc tatattctta aaattattaa tcgaaatatt attgtaattt
    22201 tcttgctgca ttatgttttt gggttttaga ccaaactcaa atatacatct atttacaaag
    22261 tttatcaaat taaaaaagct cattaaaata tgatcaatat ctctattatt atcaataatc
    22321 tctttaactc ctctattaac atacaacatg actttttcat aatcacatag cttgatagaa
    22381 gaaataatct tattttccat gtcaagcgga taaaacgatt cgctatcttc agaataattg
    22441 atactacaga tatctactat atctcctgca ataaagctat acttaatggc ttttaaagat
    22501 tccttatatg aaagctcaat tttcaggata tcatcatata ttcttcctat acccgcaatt
    22561 atttcataac ctaagtagta ttctatcctc tttttaattg tttcaaaaca ttttaatatt
    22621 aaagaatcaa gcctcttttt attagcacca tcatgtatct taataagtaa aagaatatta
    22681 tcatctatat cctgggaaca tataatatca atcttttcct ctgtaaatga tttttgcaca
    22741 atattaacca ttgccagctt tttagtctcg tattcctcta tgtctacatc ggcattatac
    22801 aaacttacat ttttaattgc tgcaacctga taatattcat agttaatatt gatgttaaaa
    22861 tagcccgctt tcttttcaat tagctttctg tcatttattt tacctgttat aagattaatc
    22921 aagaaatatt ccctgaatag cagcctgttt tcatctaata gtccttctaa gcttttttta
    22981 tccttaagca gtaaattcat tccatttttt atcactgaaa tatcactatt atcacaagca
    23041 ttattaatac cactaatatc attgaaacta ttggtataat ttgtataatt ggtataacta
    23101 gtatcattta tatcaatgct tttcagacct gcttctgatt ttaaaagcat tataattgct
    23161 ttaattggct tgtatatttt tttagataat aataatgaag ctattgctat aatgataaga
    23221 atcgaaatta gtacaagaat agtaataaga cgtatttcca aagatttctt aaatataata
    23281 tttgaaggta tcattatata aagtttccaa tcattaatac caacatttga acaatagcaa
    23341 accattttac gtcctttaaa aatcatatta gtatttgctg gctcctgatt aatagaagaa
    23401 gattgactat taataatttc cttaatattg ctatataaat attcatcatt atttgatgat
    23461 aatactgtac catctccact tgctagaaag gctactgatt cactgtcatt aataatgttt
    23521 tttagtatat tggaaacaat gcaataatcc acattaatga caattgtacc aagtttctct
    23581 tcaccatcat atgggattgt ggtaagaaat gatataacat tatttttata actgctgctt
    23641 cgggaattta cactattatc gagctttctg gtatttatta atattgaatt ctccgtcatc
    23701 tgttcatagt actctttcca ccccatatca tagaaaatat gctcctcaag tggtgcagaa
    23761 aagttcagta tcatattact gttctcatag taaacaaaaa tatcattaat ataattgctg
    23821 gaattaattg cgctggataa aatatccatg agctttttta cagtgtaata gtcatcaaaa
    23881 aattcatctc ttttcaggta tgcaagctgg ttgaagtcag gatgcaatgc ataattttgt
    23941 ccaatctttt caaactcctg aaacatcatt ctaatagtat ttgcggtttt ttcaacctga
    24001 cctttctcaa atttttctat ttcctggtca aattgcctca ttatataatt atggttaaaa
    24061 acaccaacta ttatagttaa gaataaagat atggctatac acactatgaa cattttcaag
    24121 taaaggttta tgcttctcct attaaatatt tttcttaagt agcctttact attcacatat
    24181 tttcactcca atttgataaa ttgcataata atacataata atacataatg gcaaaatatg
    24241 gtacatcatg cattaagtct gataataata ttcagtcaat tccattctga tttattacca
    24301 ttatattaca cagttattgt aataacaagc ttcatttttt tacattctgt atatgttttt
    24361 gatatattgc aaaacaagtt aaaaaatata tgcatatata gataaatatt atttatatat
    24421 gcatatatgc tgaaatatgc attaatatta aagccacatg ctctttaact cgttaacttc
    24481 catcgaaata acatttatat cccctttgga agatgtcaac ttaattattc cttccctctc
    24541 atccctgata gcacccatta ccatataaac taatccatca ttgccaaata tctcaatgga
    24601 tgttcgatca ttaataatcc ttagttttaa tctattattg attgctttta actggacaga
    24661 ttgatccagg catataagca aacttttttg tttaatatat tcaatattaa tatcacatac
    24721 attcaatgaa aatacttctg cttcttctag gttaagctca acttttacat ccagtaagtc
    24781 agttttcatt tttataatcg aattggaatt ggcgtcaatt tttctcattt caaatgtatg
    24841 acttttatta tatagttcct ctatttcttt aacaggatta gtaaaaagcc gtattccctc
    24901 atcagtcgtt cttaatgata attcacacgg aaaagtcata gactgattga atggcatatc
    24961 cggtatatta tattttaacc atgcaatctg aatacatcgc ccatcgtcta tgggaagacc
    25021 attccatgtc tgtgccgcat agcagtttgt cccagaatgg gactttaaat attcactttc
    25081 ctttttaaaa ccataatcct caaggcttcc aactatatat gtagtgtttg ctccccaaaa
    25141 aacccatttc ctattgtttg gatcattatc aacccgcagc tcaaaaatat ccggacattc
    25201 tgtacaattt ggaagagtaa tttcgctaat ttttgtccat tccttaagat tgggagaagt
    25261 tagcaaagca tagtcatttt tgtccatata cagagccata acccatttac ccattgattc
    25321 attccaaata acttttggat ctctattctc attaatcagg ttccttaaaa ctggattttt
    25381 actatatttt acccatgttc ttcccttgtc gttgctataa gcaatgcact gggtaaacaa
    25441 ctgccctttt gagatttccg aaaaacctcc ggctgcagta taaatcaata caagtacttt
    25501 ctcgtcacat atctgaaatc cggaagtgtt attccaatct actgcacctg atcctgagta
    25561 catcgttcca agattatccg ggtaaagcgc atctcccagt tcttcccaat gaactaaatc
    25621 tttgcttact gcatgtcccc agtgcatatt tccccatctg catccatatg gattatgctg
    25681 atagaaaaga tgatattcgc ctttataata tataagtcca tttggatcat tcaaccagcc
    25741 gcgttttgaa gaaaaatgca cttgaggacg atacttttcc atatataaat catctgcaat
    25801 catagaatct tcctggataa tacaatctaa atcacactgt atatcttctt tatttccttt
    25861 attttcttta tttgttttat atgctttatc tgctttatcc tttttatcct ctatattcgt
    25921 cacagataca tttaattttc gtcctttata cttatcaaca tctaagaata cccaaaactc
    25981 cggttcacct tttgccattt gaatttcaga ttcataaata atgcagtcat ctataataaa
    26041 ctttaccagc cttttggggc cttttgtatc aactggaaaa ttcaaataac gtttttcaac
    26101 cagtaattct tttgataaaa tcaaaataac caactcctta tttatcacta aaaattaata
    26161 ctactttgtt atgttgcaaa tggaaagcgc atttctggag ggtaatcata gtagtattaa
    26221 tttgtttata ttaaaatctt ccagacttct aattacataa tcggcatctt tgagaatatt
    26281 actatcacct attcccagtg catacatgca agcagccttt atggccttaa ttcccgcctc
    26341 tgaatcttca actcccatgc attcttcaga gcgtacttta aggttatctg cagtagtcaa
    26401 aaaaatttcc ggatcaggct tacctttttt tattttactt gcatcagcaa tataatcaaa
    26461 gtatttgctt attcctaatt tatcaattat caaaaaagca tttttacttg cagaagcgag
    26521 tcctgttttt atctttaaat ttttcaaaag tttgagtgta ttcaaagcac ctggcaatat
    26581 atcatctgct gttatggttt cgagtaaatt tctataataa ttatttttcc tgtcagtcaa
    26641 tgaaagtttt tcttcatatg tatactgttt ttcagagttc ctcaatatga tttcaagtga
    26701 tgacattcta tcaacacctt taagtagctc gtttattttt ctgtcaaagt atacatttat
    26761 ttcgtcggtt agttttttcc acgctgcaaa atgaagattt gcggtaccgg taataacacc
    26821 atcaagatcg aatattacag cttgcaactt aatcatcata ttttccatac cctcccttat
    26881 aaatattaca atattatctc cttgccaggt tcagctgccg gcatcgttag cggtgtccaa
    26941 tgcgctgtta attccctttt gctcataaaa atacaccacc ttgctcatag atatatttac
    27001 catacagata gtgatattta atagcacgca gaagacttgt atcctcaggc agatcctcca
    27061 gcttctcaat aaatggctta ttaaatacaa atgaaaatac tgccttgcct gttgcaaatc
    27121 gaggatataa tagatatcta tgttgaaatc gtacaatgcc tgaattctcc gatagaaact
    27181 gtctaagctg gtaatccatc atccatgaag
//
LOCUS       DAOSDD010000026        26498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_845_length_26498_cov_5.859660, whole genome shotgun sequence.
ACCESSION   DAOSDD010000026 DAOSDD010000000
VERSION     DAOSDD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 26498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26498
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_26498_cov_5.859660"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PK733_04050"
     CDS             complement(<1..931)
                     /locus_tag="PK733_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09749.1"
                     /translation="MGLKDTVKRISKNVLNQALNKVLRSCAKILLPYLLAVVLIVVIV
                     VMSIGGVSMLFKSDSNNLKAEIGIIKSMPDIDIIAQQSLINPKLLDKYIDAETTNIPD
                     TLKSKSYTIINGVTSEETYTLHPYDTAYPYRLKWQLLASLDSLDTPFTETHADKSTNK
                     IRNKVTKRYTNALSQLAPIFEWGHDEYYQIVVEEIETVTEEKYKSTYTTNTTTEVITK
                     KYPLPLLKSVKTAFMEYTFTPKEKDILISDTGWATIHTETEDIIEVKKTEKTNEETNE
                     ETSEPEKEIVGWETTSIRRRLRTFEDTQSCTQIS"
     gene            complement(951..>1397)
                     /locus_tag="PK733_04055"
     CDS             complement(951..>1397)
                     /locus_tag="PK733_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09750.1"
                     /translation="GKVNAISKIASGMIGIPLAIAAAPLGQDMVKGAIGVSKVIGGSI
                     RAMGTTGALVSLSASEANSIEDFKKNYTELMNSTTNKGTLMKTGSFIKNNTLGNSEKT
                     QDLFNIISPATNKQFIRWNYAPVTDDVDKFRWADKRLSPHTIKNNL"
     assembly_gap    1398..1497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1498..2486)
                     /locus_tag="PK733_04060"
     CDS             complement(<1498..2486)
                     /locus_tag="PK733_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09751.1"
                     /translation="MNSEYFKELINNSIKPIGAAFIFLGIVTVALQIILTAHNPKERS
                     KHISSLKYIGIGACILGLTMTIYSIISSIAAPLFVPGTADPLVSDEVATKIIEEKSDW
                     ISRLIASPIVLINEGIQTVLSGNVLENFNLHTALFSETVTSGNNGHWFLSIPLSTAKY
                     LMYSLAIIVLPIIIIAIIRVAMLYIASPFNITMRREATESIYRWILSVVLIAASPMIV
                     SSLIQLNNGLVGGLSTLINKNLPNDKLTLDPVIYTGSALLTAFVQLFFTIMIFKIVFM
                     FAVRKIMILIFYAFTPIAALMWAIKKDVAAAGIWMGEMISNIFMQFFYALSYTV"
     gene            complement(2672..5077)
                     /locus_tag="PK733_04065"
     CDS             complement(2672..5077)
                     /locus_tag="PK733_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ09752.1"
                     /translation="MQKTREMRKREFNKKEIGILYKHFILQAIAVLLVPVSLPIIKSK
                     YTWLYETMINALQKVDAYMSNMYISLSAIVSRFHETEAIWFIRVTVDIILLIVGLIQK
                     LFSKIYIGSFGRLIMKVNDHFNVNTQVNIINATTINFLLVIILSYLSFIASIHLGLWI
                     SSIADKKIGFSKKSTVDNVVLGITYISMFLMLFPTTLTMYITVIPLEIILSAIRRKNY
                     STVSNTELSIESTDPSIANIQMQAVTMNLSAENLDDIMDMDDFIGREKEVQLVKDHVQ
                     KMLLYKNKSRKTASDNAPQLRQDLLKIPPFHMIITGNPGTGKTEFARAIGKYLYEMGV
                     TKKGRVVEISRHNLSSIYVAGALNNLNKYIDEAKGGVLFIDEAYSIIGGSKDTNNDML
                     SAILKIAEDKNSEIIMIFAGYKDEMTKFFNENKGLARRFHMKIHFEDYPAEILYEIFL
                     DYVKKEGFDITEDAKIAVMNKIKLIEKSKTYDFGNAAAMKELLDKDILSNLYKNRNKR
                     QNKKLITPEDIWSENEILDNSKTADDIMSGLNSLVGLTNVKVHIMRIYNDMRLKTLRA
                     ESTSSIVESSSYHCIFTGNPGTGKTTAARLMGKMFHDLGVLKTGHVVEVTRADLVGAY
                     IGQTEQKVKAILEQARGGILFVDEAYQLYVEGSSNDFGHQALQTIMTDMENNRFNYIV
                     IFAGYQDKIENMILNCNIGFASRFKHIIHFADYTDKEMQDIFCKLCKDGKMVLTKEAK
                     SVLPEKITLLRNQAGFANARSVRNYYEEVISRMNTRLSNVLDTEGEKKVKQIINKIEA
                     EDL"
     gene            complement(5283..7148)
                     /locus_tag="PK733_04070"
     CDS             complement(5283..7148)
                     /locus_tag="PK733_04070"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ09753.1"
                     /translation="MKILKPKESVDKNKKVSIDDNVLDIFDIIVPDTLKETDNNIYLG
                     RNIYSRTYAIATYPREIYVGWLDEFFQLGDIRISAYIENIPDNLVISTLTKKVVSAQS
                     QLYIYETRGDIHQIPILRTVISDLETVREAIQTNRDRMFIAKIDITVFAKTEEALEEK
                     CYMLENICSRKAMFPRSYALRQKEAFVNSLPISNITIRDFDRNFTTGSAACIIPVSNA
                     DISHNTGIFLGYNFQTNSPMFYDRFIGPPHLSTPHIYISGKSGSGKSVTMKLLTSRSA
                     IAGGIKSIIFDPENEYENIVRGLMGGEHITIRTGVKTGINPLEIEEELDDNGRYVVDI
                     YSKISFIRALMNSYLRQFAGRTLLPIETTALEEAIKEIYTQDYKITSDPASLYESGGI
                     KTEAGDYKIGKIKKKMPTLSTLHNKIKSKRNCEELYESMKPLVGDGSMSMFDCESTIK
                     LSDNLITSFNLKEIRDEFTKFYTTFILLDYTWTKFVQKDKERKSVIIDEAWLFIKYPE
                     SAKFLEEIARRGRKYHASLVVATQNIEEFILREEGRAVINACSSQILLQQNPQVVSDI
                     VKSFKLSEGCASLLETFAPGEAIVVMEGTATAVRITPTNFEWEYVTTNPLELERS"
     gene            complement(7114..7974)
                     /locus_tag="PK733_04075"
     CDS             complement(7114..7974)
                     /locus_tag="PK733_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09754.1"
                     /translation="MIVTIVFLVLTAMVIIGTAVYLKMYSPEAKNKRKAAEIGKKASV
                     NEQEIEDKYTKKEKSEIKKIWGIEDIHDGIIYLTQNRYRRIIAMGSLDFHLLSQAEQS
                     VVEEQLMQTAMSLQFPVQFITTTERINTQDTIKQIDKVIDADELYNEKTRGYASKMRL
                     YLNKMMEDRGVYVRRSYAVVCSDDMYDKQKSIGALDRYTGTIINALARAKINCIPLNT
                     TQIIDLLYRELNKGSSIVPSKVLSKGGLELYSTGLGVVEEVNLDEVQLYIEESEDTPH
                     ENIEAQRVSG"
     gene            complement(7983..8312)
                     /locus_tag="PK733_04080"
     CDS             complement(7983..8312)
                     /locus_tag="PK733_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOJ09755.1"
                     /translation="MRSFPIPFDINTEEKIVGGYLTLKQTIYLVLTVVMVLIILFVPI
                     GGGLAEMIAGKASSIAIIIKIIMCVAVSIIGVLFMFLKPHGMSFLKYLTVKIQYIDRN
                     KNIIYRR"
     gene            complement(8397..8744)
                     /locus_tag="PK733_04085"
     CDS             complement(8397..8744)
                     /locus_tag="PK733_04085"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09756.1"
                     /translation="MKKYIKKSSFIATSILLTFLLTLLTPMTAFADIDKKTARDLSNT
                     LIDTVKSYALPLGGAIIFIAVVIIAISIIINANNPKGRAEKISSLLWVGIGAAVLGLA
                     TIITGVLLSAFNI"
     gene            complement(8766..9068)
                     /locus_tag="PK733_04090"
     CDS             complement(8766..9068)
                     /locus_tag="PK733_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09757.1"
                     /translation="MYSKTLLIPEIQPVSPQEFTTRITKGLYALYGYISSLAVPLAIV
                     ILSIGAIFYIASSLMRSGTGKKIGLASMLVALSGLMLFWGIPTIVGIAQHISNIMK"
     gene            complement(9105..9455)
                     /locus_tag="PK733_04095"
     CDS             complement(9105..9455)
                     /locus_tag="PK733_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09758.1"
                     /translation="MEIKKNKIKDLNFRSIKITRHSIEQYYKRVEQIDETAVKDKLLE
                     VLENGNIVTFKPTLRGKETNKYDLHIEHDGIGITAVYQEDVITIVTCTGSKEYRKWFK
                     QQEIKLPRHIRRAA"
     gene            complement(9484..9798)
                     /locus_tag="PK733_04100"
     CDS             complement(9484..9798)
                     /locus_tag="PK733_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09759.1"
                     /translation="MHEKVKSIDYYTENWYAMLVSILKNSTVDNALEDMGVNSSSNNK
                     KKKVFYKNVTENDVIEVINLLEDNPDITLREACEIKELKGITLGAMYQRICRYKQKQK
                     LG"
     gene            complement(9992..10234)
                     /locus_tag="PK733_04105"
     CDS             complement(9992..10234)
                     /locus_tag="PK733_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ09760.1"
                     /translation="MPAIMKLSEAIKEARKAKRHSIRAMAKESGISKSYITQIQQGEQ
                     FNPTLNVIEKLAKYLDVGINEIIEMLKENGITEKSD"
     gene            complement(10541..12235)
                     /locus_tag="PK733_04110"
     CDS             complement(10541..12235)
                     /locus_tag="PK733_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ09761.1"
                     /translation="MDCFKKIIIKEVTMKGFKAFKDSQTFKFNDQVTAIYSDNHTGKT
                     SLLDSIVWAFIGTDKMGETRDTYLMNDECNSMQVRVIFESEQGRHILERKKSGKTLSI
                     LLDELPLTQAQMESICYSKHLFLSIVNPEYFVAWASEYKKDAQGLLYSILLDIPQEKI
                     MKRLDSQSKELLAGKNLNDVPLFLRNKRAELSEYEKDILHITGQYDVLTKLMDDASND
                     LDISSIDNEISKLNRLLNKYNNNADTINNQLENLMKKKANLIEEKALLHKDEAIDKFK
                     NELLIKNNQLETYRKTYADMAKEANKLKPGLKCNTCQQVITEEHVAQIHATTGKKLKD
                     ITVEAKELANEIETIKKKLLVSEKENADKIRKLDVEMEYIDIKINAIKNDSLKIKKEN
                     TETKDSILAKIIKLQETKSKIKNKAVTAKELERIKTMLQKSEQNKIQTEKIIQAGIEY
                     SYQKSEMLMSGLNDKMRHTKIKLQEVIKSTGEIKECFKLTYNSRNTILISTSEKIKMG
                     AEISKLLRELLGVDYFMILDNAECLTIFKPVASQTIAAMVRENTHLKIDGKELEAA"
     gene            complement(12349..12768)
                     /locus_tag="PK733_04115"
     CDS             complement(12349..12768)
                     /locus_tag="PK733_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ09762.1"
                     /translation="MNKAILLGRLTKDPELRYTINNVAACSFTLAVSRKYSKQDDPVT
                     ADFIPIIAWRQTAEFCSKYFQRGQQVAVVGRIQTRSWEDNEGKKHYVTEVIAEETFFA
                     DSKKDKNETGNYEKGFNPDEMPTTQFLGLDEDDDLPF"
     gene            complement(12987..13994)
                     /locus_tag="PK733_04120"
     CDS             complement(12987..13994)
                     /locus_tag="PK733_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOJ09763.1"
                     /translation="MEKYRKIIEITRQMAEKNETVTMYACIKEKQVKFKNNNSSEKYF
                     DINLIDGTGTINLKVWNYDEETDKLKIGDIVKLKAKASLYKDNLHLVLVYEQNKNYIK
                     LAPKNEIDITSFVETVEDITPKTMKQQYFHIINNQLKNAYLKKLLNLIFSNPNINTKY
                     FSMYAGKNMHHTYLSGLARHTIEVYQYANIISDTNKLPGETKEIISTIALLHDIGKIE
                     EYGDIPFMEITEKGRLFGHSYLGTVIVKAHIKTIKEFPTQLEDILLHGILSHHGTLEN
                     GSTVLPSTIESIIVSNADNVSAKTEGILDYIVKDKNESNITEYNNYLKQFFIKTMPES
                     A"
     gene            complement(14028..15386)
                     /locus_tag="PK733_04125"
     CDS             complement(14028..15386)
                     /locus_tag="PK733_04125"
                     /inference="COORDINATES: protein motif:HMM:NF039653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HOJ09764.1"
                     /translation="MSDVLYVDVKIDECVRSSEKQVANMIRLHEKIIKKYDKDLETEL
                     NAEKTKLYNNVLSLKLYLDEMEILRLSKASISILKALELFKVLTPDYTKFSEVINKYL
                     SSIKKRNEIDSNAAGRLWNNIKMGYYPTDRDNLAHIKRAITFPSSTISNILDPCCGCG
                     KALSYLAEWTKCKTYGIELDEIRAEEARKRLTRMGKGSYFGSRISHEAFHMMYLNPPY
                     LTVYSDGHNVRSEKKFLVETLYHLMYGGLLIYVIPYYRVTPDIARILSDNFDNISIYK
                     FTESEFKKYKQIAIFGVRKKKESNQNIVNMLVKQATYPELIPDVTMLKTGQYKIPAAE
                     AEVKIFKGAVFDVAELAEQLNDTKMIEDLFIENLLDNTEKRPLIPFTIGQIGLIGGSG
                     LIDGYIDCSYPHILKGQIVKERKITDEKEDFNGNTTDVTEQIINKMNLKVLTEEGVLT
                     LV"
     gene            complement(15612..16445)
                     /locus_tag="PK733_04130"
     CDS             complement(15612..16445)
                     /locus_tag="PK733_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09765.1"
                     /translation="MSISVLEQIQNKEKDAWIAIYESRQTNKILREQVVAVEEHGDAP
                     CLVVFCMDTVKVIIPLALKGGMTYEDEKKNKNMLYAMIGSNIEYVIEEIDREGEIAVG
                     NRAKAMGIRRKEIPELEVGQTITCNILAVGRKMVIAEYYGMESKIGYQDITWSFVSDL
                     RNIISVGNELKCVITEIDAENEILNVSIKKAIPDPYINIAKRYVVGGKYIGVVSLINE
                     GFTFVRLPGGVDARCPHPGWTNLKLNTQDKVQLRIRHIRPEKRSIIAVIDRLIERNNE
                     I"
     gene            complement(16491..17381)
                     /locus_tag="PK733_04135"
     CDS             complement(16491..17381)
                     /locus_tag="PK733_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09766.1"
                     /translation="MEKLICPNCMISLKKSQNALTCSKCYLKINKIYSGYTLKENDII
                     SLLEKGKTEEIEFTSKKGKKYTTFLAIINNRVNYGFNIEKKDIESQNQDPVPDTVYAR
                     IESPIPGSVNMEIKGLQKLFYQTIKFGLASTRESECLGAIVAAQYIKWALNTAENISI
                     NFSLNSKEFTQYILKENTPRDPELRSLIAYLWGYLLNFRSWTAVYNPREKQILEGGNT
                     SNVFPRGIFPWLEVESIEGDSYIGAILPQNPAVIKQFKASFNNAKIKDHPDKENKLIG
                     MLPKKADKALRTWIAMVSNQ"
     gene            complement(17314..17670)
                     /locus_tag="PK733_04140"
     CDS             complement(17314..17670)
                     /locus_tag="PK733_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09767.1"
                     /translation="MANILIEDITNSQALKKAFNDLYSEYSKEKDMRELKSRLAFIEV
                     IISNYIKAIKNTANDIKDPSLYRMETHEQIIRFFCDMEMANYLKYEKGGETLYGKIDL
                     SELHDFIKEKSECLNL"
     gene            complement(17704..19638)
                     /locus_tag="PK733_04145"
     CDS             complement(17704..19638)
                     /locus_tag="PK733_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HOJ09768.1"
                     /translation="MKKIIGVILIAILSLSGIGILAGILVQLFISSQAWTIGKEISYT
                     AISFKPEIILGHMFTKEGLIAWALLTAIIFIAIAFSKLQYASEKTDERGVKYSSDGTF
                     GTAKWMDEKTVNKVFDVTTLSKCNNIMFGEYNKKIISQSGKTFGNKHIIVFGASGSGK
                     SFSFVRNNILQVAKMGQSMVLTDPKGELYQDMVVYLESIGYNVKVFNLVQPTHSDRWN
                     PLSEVSGNNIDLKAQLFSDVVIKNTRSGTKVSGDMFWDRAEQNLLKALAIYIATEYPP
                     DKHNIAELYKLLSSSDSTMVDMIFEKLPLTHPAKAPYNIYKQASDNVRTGVIIGLGTR
                     LQVFQNQTITAMTETNDIDLESPARERCAYFCIMSDTSSALDYLASLYYSFLFTRLIN
                     YADQNGGKGEKEVYFMLDEFANIGEIPDFVKKIATMRSRGLHCHIIIQSIAQLKEKYY
                     NEGSEIVISNCDTKLCLGCNDISTARFMSDSIGTATIKDYGTTKQLGLEGVFDYGRQT
                     VREGKRYLLNPEEIMQLPQDIAIVMVKGNNPLLVKKVGWNKHYLYKKTSPCKISDYIP
                     HWVEKNKPSEIGADVFDKISAKSKAIIEPISENKDEAITEIIYAPKGFKNYAKEPNKK
                     AINTIKQNKKVKTNNDSFFM"
     gene            complement(20025..22169)
                     /locus_tag="PK733_04150"
     CDS             complement(20025..22169)
                     /locus_tag="PK733_04150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOJ09769.1"
                     /translation="MDDREFIDFLKNKYNINLNEQQKQAVLHKDGSMALISVPGSGKT
                     TVIISRTANLIINHKINPKSILTLTFSRASAQDMKHRFTKTFGELCGVEAHFSTIHSF
                     AYSVIRDFYRNRQMPLPTIIESNSDNDVKVTKTTILKQIYYDINGDYINDDKFDDLTN
                     TINYIKNMLYRPQNNFNGMEIDVPKFKEIYIEYERQKRENNYMDFSDMVMGAYDILRK
                     NANILNIYKSIYKYIQIDEAQDTSVLQNHIIRLLAYPEDNVFFAGDDDQCLYSMNGCT
                     PATMLAYKKVYPNIKIQLMEKNYRSTAKIVEVANKFILKNKERFDKQLATDKEAGRDV
                     TIISLKDDTEQYRYIADVLQKIENKKECALLYRANISAIPIADILDKNNIPFYIRDAK
                     PHFFNHWVTEDIISFMKLIMNIQDLEAFEKIYYKLNSYITKKMLEYLKEQVNINNDTR
                     NIFDILMDAPEVNVRHIKRFQKLKKVFASLQDKSAEFVINYIEFGFDYGGYLQRACQD
                     IGYSIEYLNTILSTLKSISTGIETLPEILSRLTYLKEIIENSKNNKYKNAVTLSTLHS
                     SKGLEFDNVFIVDMIEGCFPTYKAISEEKDNNLHLMEEERRLCYVGITRARKYVDIVN
                     IEYKNKLKVMKSRFIFELESIIKSNTIVKKDTSINEFESFKLMEGITIKHKKFGEGII
                     VSIEKNIIIVIFKAHGQKKLLLQHCLEHKLIS"
     gene            complement(22169..22345)
                     /locus_tag="PK733_04155"
     CDS             complement(22169..22345)
                     /locus_tag="PK733_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09770.1"
                     /translation="MRKNINTTINEELYKEVRILAIKLGLNTNDLIEEGMRYILNKYK
                     EKDINNQDEKGINT"
     gene            complement(22519..24129)
                     /locus_tag="PK733_04160"
     CDS             complement(22519..24129)
                     /locus_tag="PK733_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOJ09771.1"
                     /translation="MIKKLTNISKLLIILLIGMFIMLLTNTGLAQNISVNNVENNDIS
                     KTELDNYKILNEVYQNYKKGIYTTEFYTSSIPHNDLAITIGESFRMLSYYATLHSITL
                     NHVDFSLSDAQKIKIHINWNHYESAKRGYNEADHEVVKEIYKKEMIKYEDRNYTERFN
                     TLHQVADNIVKEALEYDTVREQLFYINRYLAVNCRYDSDIVEGKKPTLNSYFELDQSY
                     EAYGCLVDNKAVCLGYAKAVQLLCEKLNVPSIIVSGVPREDEESPGHAWNLVYVDGEW
                     LIWDATSRYIEGLTMDGTLDEGEYRWLTYYFLVSNSDYHNSFKLDQHCEKHLPYIKAI
                     ACPESSTIDLENTSIILTETQLKGYYEQKKVELERRKANGEAANPTTSNIILNGKEVK
                     FAAYNIQGHNYFKLRDLACAINGTNKQFEVLWNQQKNSINILTRSAYASVGGELSGYK
                     TENKDYVTIISPTSILDNFKSNEIEYAESTLSKLYVDDRLTSLKAYNIRDNNYFKLRD
                     IAKVIDFNVEWDGKNNMVIIDTNKSYKD"
     gene            complement(24247..25338)
                     /locus_tag="PK733_04165"
     CDS             complement(24247..25338)
                     /locus_tag="PK733_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HOJ09772.1"
                     /translation="MGKHGIDLLEYDIVDIARDLGLRIKNETHTTKEVAACCPFCPSG
                     DTKYHLNLNRFINVFRCPKCGTHGGILHLIAYLKEVDTKEAYKMLKSGDGIYKSTSDN
                     ISYKETKRRQYLDEEQNAKSISERHKVYTEFLDMLTLSDKHKTNLLQRGLSKKTIEEN
                     QYKSIPQVDDIRKRICWKLSLKYDLKGIAGFFSDKYNDWDFYSNSGFLIPVRNERREI
                     QGMQVRIDNSNKRKYRYFSSANYENGTSAKSYIHIVGTPVNGQIRLTEGPLKADVANY
                     LSGEPYVAIPGINSYKFLVPMLKLLKVHTVIEDFDMDKYTKEEVRVGVETVKKILQQE
                     NIKVISAKWDSRYKGVDDYLLSLYTVATI"
     gene            complement(25496..26362)
                     /locus_tag="PK733_04170"
     CDS             complement(25496..26362)
                     /locus_tag="PK733_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09773.1"
                     /translation="MEKNLVALEKGRLMAQLIEEELGGQISFSAFKRVKLPSGGGLSF
                     EIQSGENPEICTEIVGVVIDHYPIKTYYKSKNVQAGVKPDCSSVGGIEGIGIIGGLCA
                     NCPKNRFIPDVGKECSDKHRIFILRQGDTFPIYIDIPTMSITNWRKYITRLVTTPIRK
                     TDVVTKISLKRAVGNLSKATYSQVTFAAIQKLTPETAAEMLAYSKGMQKMTRSLGGVF
                     EDENEEENNNGEVVVVKEHLSSDLETDTLDTKETNASGEIMDEDIDEDILEEFNGEFD
                     GTLTEFNETLTW"
BASE COUNT         7345 a         4764 c         3643 g        10646 t
ORIGIN      
        1 ttgatatttg tgtacatgat tgtgtatctt caaatgtgcg tagtcgtctt cttatgctag
       61 ttgtttccca gccaacgatt tctttttcag gttcgctagt ctcctcatta gtctcttcat
      121 tagttttttc ggttttttta acttctataa tatcttctgt ttctgtatgg atagttgccc
      181 agcccgtatc actaataaga atgtctttct ctttaggtgt aaaagtatat tccataaatg
      241 cagtttttac agattttaat aaaggtaatg gatacttttt ggtaataact tctgtggtcg
      301 tatttgtagt atatgtactt ttgtattttt cttcagttac tgtttctatt tcctcaacta
      361 cgatttgata atattcatca tgtccccatt caaaaatagg tgctaattga gataatgcat
      421 ttgtatatct tttagtaact ttatttctta ttttattagt tgatttatct gcgtgagttt
      481 ccgtaaatgg ggtatctagt gagtctagtg aagctaatag ttgccatttt aacctatatg
      541 gatatgctgt atcatatgga tgaagtgtat atgtttcctc tgatgttacc ccatttatta
      601 ttgtatatga ttttgatttt aatgtatctg gtatgtttgt tgtttctgcg tctatatatt
      661 tgtctaataa tttagggttt attaaagact gctgtgctat aatgtctata tctggcatgc
      721 tttttataat acctatttct gcttttaaat tattgctatc tgatttgaaa agcatagaca
      781 cgccgcctat tgacattact acaattacta cgattaatac aacagccaat aaataaggta
      841 acaatatttt agcacagctt cgtaatacct tattcaatgc ttgatttaaa acattttttg
      901 atattcgttt tactgtatct tttaacccca tttattcata aacctccaac ttacaggttg
      961 ttttttattg tatggggact taacctcttg tctgcccatc taaatttatc aacatcatct
     1021 gtaacaggtg cgtaattcca tcggataaat tgtttgttag ttgccggtga tattatgtta
     1081 aacaaatctt gagttttttc actattacct aacgtattgt tttttatgaa acttcctgtt
     1141 ttcatgagtg ttcctttatt tgttgttgaa ttcattagtt ctgtataatt ctttttaaaa
     1201 tcttctatac tatttgcttc tgatgccgat aggcttacta atgctcctgt tgtacccata
     1261 gcccttattg acccgcctat aactttgctt actcctatag ctccttttac catatcctgt
     1321 cctaaaggtg cagctgctat tgcaagaggt atgcctatca tgcctgatgc tatttttgaa
     1381 attgcattaa ctttgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca
     1501 gtataagata aagcataaaa aaattgcata aatatatttg aaatcatttc acccatccat
     1561 atccctgctg ctgcaacatc cttcttaatt gcccacatta gtgctgcaat gggtgtaaac
     1621 gcataaaata ttaaaatcat aatttttcga actgcaaaca taaatactat cttaaatatc
     1681 attattgtaa aaaacaattg tacaaatgct gtaagtaatg ctgaacctgt atatataaca
     1741 gggtcaagtg ttagcttatc attaggtaaa tttttattaa taagtgttga taaaccacct
     1801 acgaggccgt tatttaactg tataagagag cttactatca tgggtgatgc tgctatcaat
     1861 acaacagaca atatccatct atatatactt tcagttgctt ctcttcgcat tgttatgttg
     1921 aaaggtgatg caatatataa catagctact cttataatag caattataat tataggaaga
     1981 actattatcg ctaaactata cattaaatat ttagctgttg atagtggaat tgataaaaac
     2041 caatggccgt tgtttcctga tgttacggtc tcactgaata atgctgtatg taaattgaaa
     2101 ttttctaata catttcctga taatactgtt tgtatacctt cattgattag tactattggc
     2161 gatgctatta gccttgatat ccaatctgat ttttcttcga ttatttttgt tgcaacttca
     2221 tcacttacta aaggatcagc tgtaccagga acaaataatg gtgccgcaat agaacttata
     2281 atactatata tagtcatagt aagccctaat atacatgcgc ctataccgat atacttgagt
     2341 gatgatatat gctttgaacg ttctttagga ttgtgagctg tcaatattat ttgtagggca
     2401 accgttacaa ttcctaaaaa tataaatgcg gcacctattg gctttataga gttgtttatt
     2461 aactccttaa aatattcact gttcatttaa tattttgacg taatttgaat aatttacgtt
     2521 tcctccattc ttctcttcct taatttaatt taattaattg atatataaat attaataaaa
     2581 aatctctaga atattgatta tgtgtatata ataacataca aaaaaagtgt caaataaagc
     2641 tcttgaatca agaccttatt tgacacttcc tttacaaatc ttcagcttct attttattta
     2701 taatttgctt tacttttttt tcaccctcag tatctaatac attagataac cgggtattca
     2761 ttctagaaat gacttcttcg tagtaatttc ttacacttct agcatttgca aaccctgctt
     2821 gatttcttag aagcgttatt ttttcaggca aaacagattt tgcttcttta gttagcacca
     2881 ttttcccgtc tttgcataat ttacaaaaga tatcttgcat ttctttatca gtataatcgg
     2941 caaaatgtat tatgtgtttg aatcgtgaag caaaacctat gttacaattc aatatcatat
     3001 tttctatttt atcctgatat cctgcgaaaa taacgatata attaaatctg ttattttcca
     3061 tgtccgtcat aatagtttgt aaagcctggt gtccaaagtc gtttgatgaa ccttccacat
     3121 ataactgata ggcttcatct acaaacaata taccacccct tgcttgttct aatatagcct
     3181 ttactttttg ttcggtctgg cctatatagg ctccaaccaa atcggctctt gttacttcaa
     3241 ccacatggcc ggtttttaga acaccaagat catgaaacat tttccccatc aaccttgccg
     3301 cagttgtttt acctgtccct ggattgcctg taaatataca atgatatgaa gatgattcga
     3361 caatggaact agtgctttct gctcgtaaag tttttaatcg catatcgtta taaattctca
     3421 taatatgcac ttttacattt gttaagccta caagtgaatt caaaccagac attatatcat
     3481 cggcagtttt gctattatcc aaaatttcat tttcactcca tatatcttct ggagtaatta
     3541 actttttatt ttgtctttta ttcctgtttt tataaaggtt acttaatata tctttatcaa
     3601 gtaactcctt catagcagca gcattaccaa agtcataggt tttacttttt tctattagct
     3661 taatcttgtt cattacagca attttagcat cttcggttat gtcaaaacct tcttttttaa
     3721 cataatctaa aaaaatctca tacaatattt ctgcaggata gtcttcaaaa tgtattttca
     3781 tatggaatct tcttgctaag cctttgtttt cattaaaaaa tttagtcatt tcatctttat
     3841 accctgcaaa gatcattatt atttcgctgt tcttatcttc tgcaattttc aaaatagcac
     3901 ttaacatatc attattggta tctttagaac caccaattat cgaatatgct tcgtctataa
     3961 ataaaacccc accttttgct tcatcaatat acttgtttag attatttaat gctcctgcta
     4021 catatattga agataaattg tgtcttgaaa tttccacaac ccttcctttt tttgtaacac
     4081 ccatttcata tagatactta ccaatcgccc ttgcaaattc ggtcttccca gttccagggt
     4141 tccctgtaat tatcatgtga aatggcggta tctttaataa gtcctgtctt aattgtggtg
     4201 cattatcaga cgctgttttc ctgcttttat ttttatacaa tagcattttt tgtacatggt
     4261 ctttaaccag ctgcacttct ttttccctgc ctatgaaatc atccatatcc attatgtcat
     4321 caaggttttc tgcacttaga ttcatagtta ctgcctgcat ttgaatatta gcaatgcttg
     4381 ggtctgtgct ttcaatactt aactcagtat ttgatacagt tgagtaattt ttccttctaa
     4441 ttgctgataa aattatttct aatggtatta ctgttatata cattgtaagt gttgttggga
     4501 atagcattaa aaacattgat atatatgtta ttccaaggac cacattatct actgtcgact
     4561 tttttgaaaa ccctattttt ttgtctgcta ttgaacttat ccatagccct aaatgaatag
     4621 acgcgatgaa ggacagatag gataatataa tcacaagtaa aaaattgatt gttgttgcat
     4681 ttattatatt tacttgcgta tttacattaa aatggtcatt gactttcatt ataagtctgc
     4741 caaagctacc aatgtatatt ttgctgaaaa gtttttgtat caaacctaca attagcaata
     4801 tgatgtctac tgttactcta atgaaccata ttgcttcggt ttcatgaaac ctggatacta
     4861 tagcggacaa actaatatac atattactca tgtatgcatc tactttttgc aaagcattta
     4921 tcattgtttc atatagccat gtatattttg atttaataat gggtaatgat acaggaacta
     4981 ataatacagc tattgcttgc aaaataaaat gtttgtataa tattccgatt tcctttttat
     5041 taaattccct ctttcgcatt tctcgtgttt tttgcataga tttccctcct tttagaaaat
     5101 attttttgat atgatatatt gaacaatgct ttttagaaaa caaaaaggtc cagagaacat
     5161 aatttcccct gaaccttagt ttcgtgttaa aacctaagta tcttttaaaa gatactttaa
     5221 taattcacta atacaacttt tattatattt taaatttaaa gttttagcaa gacaattaaa
     5281 tattaacttc tttctaattc taagggatta gtagttacat attcccattc aaagtttgtg
     5341 ggtgtaattc ttacagctgt tgctgtccct tccattacta caattgcttc cccaggtgca
     5401 aaggtttcta ataacgaagc acaaccttcc gataatttaa aactcttgac tatatcggat
     5461 actacttgtg ggttttgctg tagtagtatt tgtgaagagc aggcattaat tactgccctt
     5521 ccttcttcgc gtaatataaa ctcctcaata ttttgagttg caactaccaa agatgcatga
     5581 tactttcttc cccttctggc tatttcctct aaaaactttg cagattccgg atactttatg
     5641 aataaccagg cttcgtcaat aattactgat ttcctttcct tatctttttg tacgaatttt
     5701 gtccacgtat aatcaagtaa aataaatgtt gtatagaatt ttgtaaattc atctcggatc
     5761 tcttttaagt tgaaacttgt tatcaagtta tcggataatt ttatcgtaga ttcgcagtca
     5821 aacattgaca tcgaaccatc accgactaaa ggtttcatgg attcatataa ttcttcacag
     5881 ttgcgtttac ttttgatttt gttatgcaat gtgcttaatg taggcatttt tttctttatt
     5941 ttacctattt tataatcacc cgcctctgtt tttattcctc cactttcata aagagatgca
     6001 ggatcggaag ttattttata atcctgggta tatatttctt ttatagcttc ttctaatgcc
     6061 gtagtttcaa tgggtaataa tgtacgccct gcaaattgtc ttaaatatga attcatcaat
     6121 gctcttataa atgatatttt tgagtagata tcaacaacat atcttccatt atcatctaac
     6181 tcttcttcta tttctaatgg atttatgcct gttttgaccc ctgtgcgaat tgtaatatgt
     6241 tcaccaccca taaggcctct tactatgttt tcatactcgt tttccggatc aaatatgatg
     6301 gatttaatgc cacctgctat agcagaacgg cttgtaagca gtttcatagt tacactttta
     6361 cctgaaccgc ttttgccaga tatatagata tgtggtgtag atagatgagg gggtccaata
     6421 aatctatcat aaaacatagg cgaattagtt tgaaagttat aacctaaaaa tatccctgta
     6481 ttatgagata tatcggcatt cgatactggt attatacatg cagcagaacc agtggtaaaa
     6541 tttctatcaa aatcccttat agttatattt gaaataggta aactgttaac aaaggcttct
     6601 ttttgacgca atgcataact acgtggaaac atagccttcc ttgaacaaat attttctagc
     6661 atatagcact tttcttctag agcttcttct gtctttgcaa acactgtaat gtctatcttt
     6721 gctataaaca ttctatctct gtttgtttgt atggcttctc ttacggtttc taaatcagat
     6781 attactgttc ttagtatagg tatttgatgt atatctcctc ttgtttcata tatatataat
     6841 tgactttgcg cggatactac tttttttgtt agagtactga taaccaaatt atctggaatg
     6901 ttttcaatat acgcacttat tcttatatcg cctaactgaa aaaactcatc cagccatcct
     6961 acatatattt ctcgtggata tgttgctatt gcatatgtcc ttgagtatat gttcctccct
     7021 aagtatatat tgttatctgt ttctttaagt gtatccggaa caataatatc gaaaatatct
     7081 aacacattat catcaatact aactttttta tttttatcca ctgactcttt gggcttcaat
     7141 attttcatgt ggagtgtcct cgctttcctc tatgtataat tgtacttcgt ctaaatttac
     7201 ctcttctaca acccccaaac cggttgagta caattccaaa ccgcctttgg atagtacttt
     7261 agatggaact atagacgaac ctttattaag ctcacgatat agtaaatcga ttatctgtgt
     7321 tgtattgagg ggaatacaat ttatttttgc cctggccaat gcattgatta ttgtaccggt
     7381 ataacgatct aaagcaccta tggatttttg tttatcatac atatcgtcag aacatacaac
     7441 agcataactg cgtcttacat atacacctct gtcttccatc atcttattta agtaaagtcg
     7501 catttttgaa gcatatcctc ttgtcttttc gttgtataat tcgtctgcat ctattacctt
     7561 atctatttgc tttatagtat cttgagtgtt tattcgttca gttgttgtta tgaattgcac
     7621 gggaaactgt aaagacatgg ctgtttgcat taactgttct tctacaacgg attgttcggc
     7681 ttgtgataat agatgaaagt ctaaacttcc catagctata atcctgcgat atcggttctg
     7741 tgtcaaatat atgataccat catgtatgtc ctctatcccc catatttttt ttatttctga
     7801 tttttctttt tttgtatatt tatcttcaat ttcttgttca tttacagatg cttttttacc
     7861 aatctcagct gcttttcttt tgttttttgc ttcaggacta tacattttta agtatactgc
     7921 tgttccgatg attaccatcg ctgttaaaac taaaaacaca atagtaacaa tcatttttta
     7981 catcaccttc tataaataat gtttttattt ctatctatat attggatttt taccgttaaa
     8041 tattttaaaa acgacatacc atgtggcttt aaaaacataa ataatactcc tattattgaa
     8101 actgctacac acatgatgat ctttattatt atagcaatac tgcttgcttt gccagctatc
     8161 atttctgcca ggccaccacc tataggtaca aacaatatta ttaataccat tactacagtt
     8221 aacaccaaat atatagtttg ctttaaagtt aaatatccgc ctactatttt ttcttccgta
     8281 ttaatatcaa agggtatagg aaaacttctc atattgttcc acctcatttt tataataaaa
     8341 atgtggcacg aggccacgtt tttatattgt ttgaagatat ttttatgtaa gaagtattat
     8401 atattaaatg cagatagtaa tactcccgtt attattgttg ctaaccctaa taccgctgct
     8461 cctattccca cccatagaag tgaggaaatt ttttctgctc tgccctttgg attgttagca
     8521 tttataataa ttgaaattgc tatgataact actgcaataa aaataattgc cccacctaat
     8581 ggtaatgcat atgatttcac tgtatctatg agggtattgc ttaaatctct ggctgtcttt
     8641 ttatctatgt cggcaaacgc tgtcattgga gttaataaag ttagcaaaaa cgttaataat
     8701 atagatgttg cgataaatga tgatttttta atatattttt tcatagttaa tgatccccct
     8761 taatattatt tcattatatt tgatatatgt tgagctattc caactatagt aggtattccc
     8821 cagaacagca ttaaaccgct taaagcaact aacatactgg ctagtccaat ttttttccct
     8881 gttcctgacc gcattaatga actagcgata taaaatatcg cacctattga tagtattact
     8941 atggccaatg gaactgctaa agatgatatg taaccatata gagcataaag accttttgtt
     9001 attctagttg taaattcttg tggacttacg ggctgtattt ctggtattaa taatgtttta
     9061 ctgtacattt tttcacaccc ttctatatgt ggtatgcaga aattttaagc tgctcgtctt
     9121 atatgcctag gtaatttaat ttcttgttgt ttaaaccatt ttctgtactc tttactacct
     9181 gtacatgtta ctattgtaat tacatcttcc tgatatactg ctgttatacc tatgccatca
     9241 tgctctatat gcaaatcata tttattcgtc tcttttcctc ttagagtagg tttaaacgtt
     9301 actatgttcc cgttctctaa tacttctaac aacttatctt ttactgctgt ttcatctatt
     9361 tgttctactc ttttgtaata ttgctctatt gaatgcctgg taatttttat actcctgaaa
     9421 tttagatcct ttattttatt cttttttatc tccacaaaca ttttccccct tcctcttttt
     9481 gtattaacct agtttttgct tttgtttata tctgcatatt ctttgataca tggctccgag
     9541 cgtgattcct tttaattctt taatctcaca ggcttctctt aacgttatat cgggattatc
     9601 ctctaataga tttataactt caattacatc gttttctgtt acatttttgt agaatacttt
     9661 tttcttttta ttattgcttg atgaatttac gcccatatct tccagagcat tatcgactgt
     9721 gctgtttttt aatattgaaa ctagcattgc ataccaattt tccgtataat aatctattga
     9781 ctttactttc tcatgcatac tatcaacacc tttcaacaat taaaatattt ttttcaagta
     9841 cctttttttt gttgaagaac gtgcgattat ctgctaaaat aggtcgtagt tgaaccatgt
     9901 taagcgtata gctcaacagt gtttaactga ttcatgtaag aattaatacc tgatacccag
     9961 tgtcccatac gctgggtatc acccattttt tttaatcgct tttttccgtg ataccatttt
    10021 cctttaacat ttcgattatt tcgtttattc cgacatctag atattttgct aacttttcta
    10081 tgacatttaa tgtcgggtta aactgctcac cttgttgtat ttgtgttata taactttttg
    10141 atatgccact ttcttttgcc atagctcgaa ttgaatgcct tttagctttc cttgcctctt
    10201 tgatagcttc tgataatttc ataatagctg gcatgaatac cgcttaattt aatttattcg
    10261 ggatgtgctg tacccccccc tgccaaatcc cacctttcac tttttaatag tagtctattg
    10321 ttaaaaaaag ctgttattat aatattgtcc attatattgg acaatatgca agttcgatat
    10381 atggcattta ttgccgatta tatgtaatat tttagatgta tcgttaatat tatcctccta
    10441 gcttaatatt ttgattttca ctctgcattt acggacttgt gaccgtcaga ttatatctgg
    10501 ttgcattaca gaagagcatc agctcttctt cttcagaata ttaagctgct tccagttcct
    10561 tcccatctat ttttagatgc gtattctctc ttaccatagc tgctatcgtt tgagaagcta
    10621 ctggcttaaa tattgttaag cattcagcgt tgtctaggat catgaaataa tctacaccca
    10681 gaagttctct gagtaatttt gatatctctg cacccatttt tatcttttct gaagtgctta
    10741 tcaatattgt atttcttgag ttataggtta atttgaagca ttcttttatt tctccagtcg
    10801 attttattac ctcctggagt tttattttag tatgcctcat cttatcatta agcccggaca
    10861 ttaacatctc ggacttttgg taggaatact ctattcctgc ctgtataatt ttttctgttt
    10921 gtattttgtt ttgttctgat ttttgtaaca ttgtttttat tctttctaat tcttttgcag
    10981 taacagcttt atttttaatc ttactcttgg tttcctgcaa ttttattatt ttagctaaaa
    11041 tgctatcttt cgtttccgta ttttccttct ttatttttaa actatcgttt ttaatagcat
    11101 ttatttttat atcaatgtat tccatttcta catccaattt cctgatttta tcagcattct
    11161 ccttttcgct gactagcaat tttttcttta ttgtttctat ttcattagcc agctccttag
    11221 cttctacagt tatgtctttt aattttttac ctgtagtagc gtgtatctgt gctacatgct
    11281 cttccgtaat aacttgctga catgtattac attttaaccc cggtttcaat ttatttgcct
    11341 ctttggccat gtcagcatat gttttacggt atgtttctaa ttggttattt tttataagca
    11401 attcattttt aaatttatct attgcttcat ccttatgcag taatgctttt tcctcaatta
    11461 gattagcttt tttcttcatt aaattttcta attggttatt tattgtatcg gcattattgt
    11521 tatatttatt aagcagccga ttcaactttg atatttcatt atctatagat gaaatatcaa
    11581 ggtcattact agcatcatcc atcaattttg ttaggacgtc gtattggcct gtaatatgaa
    11641 gaatatcctt ttcgtactca cttaattctg ctcttttatt tcttagaaat aatgggacgt
    11701 cattgagatt tttaccagcc aataattcct tactttggct gtctagacgt ttcattattt
    11761 tttcttgtgg aatatcaagc aaaatactgt acaatagacc ctgtgcatcc tttttgtatt
    11821 ccgatgccca tgcaacaaaa tattctggat ttactatact taaaaacaag tgctttgaat
    11881 agcatatact ttccatttgt gcctgtgtta aaggcaattc gtctagtaat atgcttaacg
    11941 ttttaccgct ttttttcctc tctaatatat gccttccttg ttcactttca aaaatgacac
    12001 gaacctgcat tgaattacac tcgtcattca ttaggtaagt gtcccttgtt tctcccattt
    12061 tgtctgtacc aataaaggcc catactattg agtctagcag gcttgtcttg cctgtatggt
    12121 tatcagagta tatggcagtt acttggtcat taaatttaaa tgtctgcgaa tctttaaagg
    12181 ctttaaaccc tttcatagtt acttccttta taattatttt tttaaaacaa tccattttta
    12241 tcccctttct catttttttt aaaaaatata gctatgacaa tatttttaat atataaatta
    12301 tcatagctat atatttttta tttacctgag tgtcgcaatc agatctgatc agaatggcaa
    12361 atcatcatct tcgtctaatc ccaagaactg agttgttggc atttcatctg gattgaatcc
    12421 tttttcatag ttaccagttt cattcttatc ctttttacta tcagcaaaaa aggtttcttc
    12481 agcaataact tctgttacgt aatgcttttt cccttcatta tcttcccatg accttgtttg
    12541 aattcttcca actactgcaa cctgttgacc tctttggaaa tacttactgc aaaactctgc
    12601 agtttgtcgc caggcaataa tagggataaa gtcagcagta acaggatcat cctgttttga
    12661 atattttctg cttacagcta gggtaaacga gcacgctgca acattattaa ttgtatacct
    12721 cagttcaggg tctttagtta gcctccctaa taaaatagct ttattcatat ttttctcctt
    12781 tataatattt tttggaaata aaaaaaggcc cagagaacac aattttccct gaacctttta
    12841 ttcttgtttt aacctaagca tccacgaaag gatactttaa taaataacac gtaacacgca
    12901 ctattattag tatatagtta taattgttat aagtcaacat tggattatta tggaatttca
    12961 tgttgacata tactttcttt atgctcttat gcactttcag gcattgtctt aataaaaaac
    13021 tgttttaaat aattgttgta ttcagttata ttgctttcat tcttatcttt gactatatag
    13081 tccagtatgc cttctgtctt tgcagatacg ttatcagcat tgcttacgat aatagactct
    13141 atagtgcttg gtaaaactgt agaaccgttt tctagtgtac cgtggtgcga taatatgccg
    13201 tgtaataaaa tatcttctaa ttgcgtagga aattctttta tagttttaat atgagcctta
    13261 actatgaccg ttcctaaata tgaatgacca aatagtctac ccttttccgt gatttccatg
    13321 aatgggatat ctccatattc ttctattttc ccaatgtcat gcagtaaagc tattgtacta
    13381 attatttctt ttgtttcgcc tggaagcttg tttgtatctg atataatatt cgcatactga
    13441 tacacttcta tagtatgtct agctaatccg gacaaataag tgtgatgcat gtttttacca
    13501 gcatacattg aaaaatattt tgtattaata tttgggttag aaaatattaa atttaaaagt
    13561 ttttttaaat aagcattttt taattgatta tttattatat gaaagtattg ctgcttcatg
    13621 gttttaggtg ttatgtcttc aactgtctcc acgaacgatg ttatgtctat ttcgtttttg
    13681 ggagcaagct taatataatt tttattttgt tcgtatacta atacaagatg taagttatct
    13741 ttatacaaac ttgctttagc ttttaattta acgatgtctc cgattttcaa tttatcggtc
    13801 tcttcatcat aattccatac cttgaggtta atggtacctg taccatctat caaattaata
    13861 tcgaaatatt tctcggatga attgttattt ttgaatttaa cttgtttttc ttttatgcag
    13921 gcatacattg taactgtttc atttttttcg gccatttgac gtgttatttc aattattttt
    13981 cgatattttt ccatagtttc accttcctat tttgattttt tattttttta aactaaggtt
    14041 aaaacccctt cttctgtcag aacctttaag ttcattttgt ttataatttg ttcggtaaca
    14101 tcagtagtat taccattgaa atcttccttt tcgtctgtta tctttcgttc ctttactatt
    14161 tgtcccttta ggatatgagg ataactgcaa tctatgtaac cgtctattag ccccgaccct
    14221 cctattaatc ctatttgccc tatagtaaaa ggtatgagtg gtcttttttc cgtgttatct
    14281 aataaatttt ctatgaacag gtcttctatc atttttgtat catttaattg ttcagctagc
    14341 tctgctacat caaatactgc tcctttaaat atttttactt ctgcttcagc tgcaggaatt
    14401 ttatattgac ctgtttttaa catagttaca tccggaatta actcagggta tgttgcctgt
    14461 tttactaaca tattaactat gttttgattg ctttccttct tttttctcac tccgaatatg
    14521 gctatttgct tatatttttt aaattcgctt tctgtgaatt tatatataga aatattatca
    14581 aagttatcag ataatattct tgctatatca ggagttacac gatagtaagg gataacatag
    14641 attaacaggc caccatacat taaatggtag agcgtttcaa ctaaaaactt tttttcagac
    14701 ctcacattat gtccatcaga atagactgta agatacggag gatttaggta catcatatga
    14761 aaagcttcat gtgatattct agatccaaaa taactacctt tccccatcct agttaggcgt
    14821 ttcctggctt cttctgccct gatttcgtca agctctatac cataagtttt gcatttggtc
    14881 cattcggcca gatatgataa tgctttacca catccacaac aaggatctaa aatattactt
    14941 atagtacttg atggaaaagt tatagctctt ttaatatgag ctagattgtc tcggtctgtt
    15001 ggataataac ccatctttat gttgttccac aatctacccg ctgcattact gtctatttca
    15061 ttgcgttttt ttatactgct caaatacttg ttgattactt ctgaaaactt tgtgtagtcc
    15121 ggagttaata ctttaaacaa ttctaatgct tttaatatgg aaatactggc tttagataag
    15181 cgcaatattt ccatttcgtc aagataaagc tttaaagaaa gcacattgtt gtaaagcttt
    15241 gtcttttctg catttagttc agtttctaaa tctttatcat actttttgat aattttttcg
    15301 tgtagcctta tcatattagc tacttgcttt tctgatgaac gtacacattc atcaattttt
    15361 acgtctacat ataatacatc tgacattata catcatctcc tttttatttt tataataaaa
    15421 aatagcaaga aaacttatat tattttatat acgtttcctt gctatcatca ttttaaaata
    15481 caaaaaggcc cagagaatat gatttctcta gaccattttt tctttttaga acctaagcat
    15541 ccttgaaagg atactttaat aatttacctt tacagtgttt attatataac atttattttg
    15601 ctttatcaag attatatttc attgttacgt tctataagcc tgtctatgac tgctattata
    15661 ctgcgttttt caggtcttat atgtctaatc cttaattgta ccttatcttg tgtattaagt
    15721 tttaaattag tccaaccagg gtgcggacat cttgcatcta cacccccagg caaccttacg
    15781 aaagtaaaac cttcgttgat aagagataca acccctatgt attttccacc tacgacatag
    15841 cgttttgcaa tatttatgta cggatctgga attgcttttt ttatggatac atttagtatt
    15901 tcattttctg catcaatttc cgttataaca cactttaatt catttccaac ggatattata
    15961 tttctcaaat ctgagacaaa ggaccaagtt atgtcttgat accctatttt actttccatc
    16021 ccatagtact cagctattac cattttccta ccgactgcca gaatgttgca tgtgattgtc
    16081 tgcccaactt cgagttcggg tatttccttt ctccttattc ccattgcttt tgccctgttg
    16141 cctactgcta tttctccttc tctgtctatt tcttcaataa catattcaat atttgagcct
    16201 atcatagcat ataacatatt tttatttttc ttttcatctt catatgtcat accacctttt
    16261 aatgctagtg gtatgataac cttgacggta tccatgcaaa aaacgactag acatggggca
    16321 tcgccgtgtt cctccacagc aactacctgt tctcttagta ttttattcgt ttgtcttgat
    16381 tcgtaaattg ctatccaggc atccttttcc ttattttgta tttgttctaa tacagatata
    16441 ctcatgtgtt tacctccaaa tcctttgttt tttaactaac atttttttgt ttattgatta
    16501 ctaaccatgg ctatccatgt tcttaacgcc ttgtctgctt ttttaggtaa cattcctatt
    16561 agcttgtttt ctttatcagg atgatctttt attttggcat tgttaaaaga agctttaaat
    16621 tgttttatta ctgccggatt ttgtggcaat atcgccccta tatagctgtc tccttcgatg
    16681 gattctactt ctagccatgg aaatattcct ctaggaaaaa cattactggt attccctcct
    16741 tctaatattt gcttttccct tggattgtaa actgctgtcc agcttctaaa attaagaaga
    16801 tatccccata gatatgctat caaagatctc agttcagggt ctcttggggt gttttccttt
    16861 aatatatatt gagtaaactc tttactgttt agagaaaaat taatacttat gttttctgcc
    16921 gtattcaatg cccattttat atattgtgct gctacaatag cgcctaagca ctcagattcc
    16981 cgtgttgatg ctaaaccaaa tttaattgtt tggtagaaaa gcttctgcaa tcccttgatt
    17041 tccatattta cactaccggg gataggtgac tcaatgcgtg cataaaccgt atcgggtacg
    17101 ggatcttggt tttgggattc gatatctttc ttttctatat taaaaccata atttaccctg
    17161 ttatttataa tagccaggaa ggttgtatac tttttgcctt tttttgatgt aaactcaatt
    17221 tcctctgttt tacctttttc taacaacgat ataatatcat tttcctttaa agtatatcct
    17281 gaataaattt tatttatctt taaatagcac ttactacaag ttaaggcatt ctgacttttc
    17341 tttaatgaaa tcatgcaatt cggacaaatc aatttttcca taaagcgtct cccctccttt
    17401 ctcatatttt aaataattag ccatttccat atcacagaaa aatctaatta tttgttcatg
    17461 tgtttccata cggtaaagtg aggggtcttt gatgtcattt gctgtgtttt ttatagcctt
    17521 gatatagttt gatatgataa cttctataaa tgctaaccta gactttagtt ccctcatatc
    17581 tttttcctta gaatattcgc tatataagtc attaaaggct ttttttaatg cttgggaatt
    17641 agtaatatct tcgattaaaa tattcgccac ccctttatta attaataatt aacaagatgt
    17701 atcctacata aaaaatgagt cattatttgt tttaactttt ttattttgtt ttatagtatt
    17761 tattgctttt ttgttcggtt cttttgcata gtttttaaac ccctttggcg catatataat
    17821 ttctgttatt gcttcatctt tattttcgct tattggttct attattgctt ttgattttgc
    17881 actgattttg tcaaaaacat cggctcctat ctctgatggt ttgttttttt ctacccagtg
    17941 cgggatataa tcagaaattt tgcatggaga agtttttttg tacaaataat gtttattcca
    18001 gccgactttt ttgactaata atgggttatt accctttacc attactattg ctatatcttg
    18061 cggcaactgc attatttctt caggatttaa taaatatcgt ttcccttctc ttactgtttg
    18121 ccggccataa tcaaaaacac cttctaatcc taattgtttg gtagttccgt aatcttttat
    18181 agtagctgtg cctattgaat ccgacataaa tcttgctgta gaaatatcgt tgcagcctaa
    18241 gcatagcttt gtatcgcagt tagatataac tatttctgac ccttcgttat aatatttttc
    18301 ttttaattgg gcaatgcttt ggattataat atgacaatgt agccctctgc tcctcatagt
    18361 agctattttt ttaacaaaat cagggatttc accgatattt gcaaattcgt ctaacatgaa
    18421 gtaaacttct ttttcaccct taccgccgtt ttggtctgca tagttaatta accttgtaaa
    18481 aagaaatgaa tagtataagc tggcaagata atctaaagct gaacttgtgt ctgacattat
    18541 acaaaaatat gcacatcttt ctcgtgcagg tgattctaag tctatatcat tagtttccgt
    18601 catcgctgtt attgtttgat tttggaatac ttgcaacctt gtacctaatc ctattattac
    18661 tcctgttctt acattatcac tagcttgttt atatatgttg tatggcgctt ttgctggatg
    18721 cgttaatggt agcttttcaa atatcatatc taccatagtg ctatcagaac ttgataataa
    18781 tttataaagc tctgctatgt tatgcttatc cggaggatat tctgttgcta tatatatggc
    18841 taaagccttt aataagtttt gctcagccct atcccaaaac atatcgccgc ttacttttgt
    18901 acccgatctt gtgtttttta taactacgtc cgaaaacaat tgtgccttta agtcaatatt
    18961 gtttccagaa acttcactca atggattcca cctgtcagag tgtgttggtt gtacaagatt
    19021 aaatactttc acattatagc ctatggattc taagtatacg accatgtctt ggtacagttc
    19081 tcctttagga tcagttaata ccatagattg acccattttt gcaacttgta atatgttgtt
    19141 gcgtacaaat gagaaagatt taccacttcc agaagctcca aatactatta tgtgtttgtt
    19201 cccaaatgtt ttccctgatt gagatattat ttttttattg tattctccga acatgatgtt
    19261 attacactta gaaagtgttg ttacatcaaa tactttatta acagtttttt catccatcca
    19321 ctttgcggta ccaaaagttc cgtctgatga atacttgaca cctctctcat ccgttttttc
    19381 acttgcatat tgcaattttg aaaatgctat tgctataaat ataattgcag ttaataatgc
    19441 ccatgcaatt agtccttctt ttgtaaacat atgccccaaa attatttcgg gtttaaagct
    19501 tatagctgtg tagcttattt ctttgcctat tgtccatgct tgagatgata tgaataattg
    19561 tactaaaatg cctgctagaa ttcctattcc tgataagcta agtatagcta tcaatataac
    19621 tccaataatt tttttcatca atattttctc ctaagttgaa aaacttattt atttaattgt
    19681 atcatatata ttttgatgtc aaataaccgg ttagaacctt attggcaatg aacagcgaag
    19741 gaaatataag ggggtttatt atgggcaaaa aaattaaaga agacggatac cataagtccg
    19801 gcacattgta gactgttgac cgtatcgtcg tagagtgatt atacaaaaag gagcctaaaa
    19861 tttaggctcc tacccacccg gatagattaa catttcaaaa aaaaatataa tttatcgcgg
    19921 actgtataag aaaatgttag aaagtatttt tacaatatca tggcttagta tggttgtcaa
    19981 acacgaataa tcttacacta gatacattgt tatttgaggt gcttttaact tattagcttg
    20041 tgttctagac aatgttgtag taataacttt ttttggccat gtgctttaaa tataactata
    20101 attatatttt tttctataga aacaattatt ccttcaccaa atttcttatg ttttatagtt
    20161 attccttcca ttagtttaaa tgattcaaat tcatttatag atgtatcttt tttaacgatt
    20221 gtattagatt ttataatact ctctaattca aaaataaatc tggatttcat tacctttaat
    20281 ttgtttttat attctatatt tactatatct acatattttc ttgctcttgt tattccaaca
    20341 tagcacaatc tacgttcttc ctccataaga tgcaaattat tatctttttc ttctgaaatg
    20401 gccttataag taggaaaaca accctctatc atatctacaa taaatacatt gtcaaattcc
    20461 aatcctttgc ttgaatgcaa agttgataat gttacagcat ttttgtactt gttgttcttt
    20521 gagttttcta ttatttcttt taaatatgta agccggctca aaatttctgg caaggtctct
    20581 atccctgtag atatagattt caacgttgaa agtattgtat tcaagtactc tattgagtaa
    20641 cctatgtctt gacatgctct ttgcaaatac ccaccataat caaaaccaaa ctctatatag
    20701 tttattacaa actcagcgct cttatcttgc aagctagcga atactttttt taacttttga
    20761 aaccttttaa tatgtcttac atttacttca ggtgcatcca ttaatatatc aaatatgttt
    20821 ctggtgtcgt tgtttatgtt tacttgttct ttcaggtatt caagcatttt ttttgttata
    20881 taactgttta acttatagta tattttctca aatgcttcaa ggtcctgtat gttcataatt
    20941 agtttcatga aggatattat gtcttctgtt acccaatgat taaaaaaatg cggctttgca
    21001 tcccgtatat aaaatggtat attgttttta tctagtatat cagctattgg tattgctgat
    21061 atgtttgctc tgtataataa tgcacattct tttttgtttt ctatcttttg taaaacgtcg
    21121 gcaatgtatc tatattgttc ggtatcatct tttaagctta ttattgtaac atccctgcct
    21181 gcttctttat ctgtagcaag ctgcttatca aaacgttctt tgttttttag tatgaattta
    21241 tttgcaacct caactatttt ggcagtagaa cgataatttt tttccattag ttgtatttta
    21301 atattaggat atactttctt atatgcgagc attgtcgcag gggtacatcc gttcattgag
    21361 tataggcatt ggtcatcatc tcctgcaaaa aacacattat cttccggata tgccaaaagt
    21421 cgaattatat ggttttgtaa tacgctagta tcttgagctt cgtctatttg tatgtattta
    21481 tatatgcttt tatatatgtt taaaatattc gcattttttc ttaaaatatc atatgcgccc
    21541 attaccatgt cactaaagtc catgtagttg ttttctcttt tttgacgttc gtattctata
    21601 tagatttctt taaactttgg aacatctatt tccattccat tgaaattatt ttgtggtctg
    21661 tagagcatat ttttaatata atttatagta tttgttaggt catcaaattt atcatcatta
    21721 atgtaatcgc cgttaatatc ataatatatt tgtttcaaaa tagttgtttt tgttaccttg
    21781 acatcattat cactattgct ttctataatt gttggtaaag gcatttgcct atttctatag
    21841 aaatctctaa taactgaata agcaaagctg tgtatagttg aaaagtgagc ttccacaccg
    21901 caaagttctc caaaagtttt tgtaaatctg tgcttcatat cttgtgcaga agctctgcta
    21961 aatgtaagtg ttaatatgct ctttggatta atcttgtgat ttattattaa atttgcagtc
    22021 cgacttatga taacggttgt ttttccactt ccaggaacac ttattaatgc catagaacca
    22081 tctttatgca acacagcttg cttttgctgt tcatttaagt taatattata tttgtttttt
    22141 aagaagtcaa taaactccct atcatccatt aagtatttat tcctttctca tcttgattat
    22201 ttatatcctt ttctttatac ttgtttaata tatatctcat tccttcttca attaggtcat
    22261 tggtgtttag gcctagtttg atagccaaga tccttacttc tttgtacagt tcttcgttta
    22321 ttgttgtatt gatgtttttc ctcataataa ttgttcctct tccaatatat tattctctaa
    22381 tttattattg cacgggattt ttgttaggca gacatcccga taaactgcat acttttatat
    22441 taatttccgc tcagttaatt atactatatt gtcacattgc tgtcaatata actatgtaca
    22501 tgtacaacaa tgtttctttt aatctttata acttttatta gtgtctatta ttaccatatt
    22561 gtttttgcca tcccattcta cgttgaaatc tattaccttt gctatatctc tgagtttgaa
    22621 atagttatta tcacgtatat tataggcttt aaggctcgtt aacctgtcat caacgtataa
    22681 cttagataaa gttgattctg catattctat ttcattgctt ttgaaattat ccaggataga
    22741 tgtaggactt attatagtta cataatcctt gttttctgtc ttgtaaccag ataattcacc
    22801 acctacagaa gcataagcac ttctagttaa tatatttata ctgttttttt gttgattcca
    22861 caatacttca aattgtttgt ttgtaccatt tatagcacag gctaaatctc ttaacttaaa
    22921 ataattatgt ccctgtatat tataagctgc gaattttact tctttaccgt ttaatataat
    22981 attagaagtt gttggatttg ctgcttcgcc attggctttc cgtctttcta attctacttt
    23041 tttttgttca taatagcctt ttagctgtgt ttctgtgagt ataatgcttg tgttttctaa
    23101 atctatagtg ctagattcag gacatgctat ggcctttata tatggtaaat gtttttcgca
    23161 atgttgatct aacttaaaag aattatgata atcactatta ctcactaaaa agtaatatgt
    23221 taaccatcta tattcgcctt catctaatgt tccgtccatg gtgaggcctt ctatataacg
    23281 actagttgca tcccatatta gccattcccc atctacatat accaaattcc atgcgtgacc
    23341 ggggctttct tcgtcttctc taggtacccc agatactatg attgatggta catttaattt
    23401 ttcgcataac aactgtacag ccttagcata gccaaggcat acggctttat tatctaccaa
    23461 acatccatac gcttcataag attgatcaag ttcaaaataa gaattaagag taggcttttt
    23521 accttctact atgtcggaat cgtatctgca atttacggcc agatatctat tgatataaaa
    23581 taattgttct ctcacagtat cgtattctaa tgcttctttt actatattat ctgctacttg
    23641 atgtaaagta ttaaaccttt ccgtataatt cctatcttca tatttaatca tttctttttt
    23701 atatatttcc ttaactactt catgatctgc ttcgttatat cctcgtttcg cactctcata
    23761 atgattccaa ttaatatgta tttttatttt ttgagcatct gataaagaaa aatctacgtg
    23821 attaagtgtt atgctgtgta gcgttgcata atatgacaac attctaaagc tttctcctat
    23881 ggttatagct agatcattat gaggtattga gcttgtataa aattctgtcg tatatatgcc
    23941 ttttttataa ttttggtaaa cttcatttaa aatcttgtaa ttgtctaatt cagttttgct
    24001 tatatcgttg ttttctacat tgttaactga aatgttttgt gccaatccgg tattagttaa
    24061 aagcattata aacattccta tcaatagaat gattagcagc tttgatatat tcgtcaattt
    24121 ttttatcata ggtttcaccc ctatcccttt tatgtttttg caaagcacac tgctttatta
    24181 tattattaat gcacctttta tcaattaaac gaaaggtgca ttaataatac aaatatttta
    24241 tcatgattat atagtagcaa cagtatataa tgatagcaaa tagtcatcaa cacctttata
    24301 tctgctatcc cattttgcag atattacttt tatattttct tgctgcaata tcttttttac
    24361 tgtttctact cctactctga cctcctcttt tgtgtactta tccatgtcaa agtcttcaat
    24421 tactgtatgc acttttaata actttaacat aggtactaag aatttgtatg aattaatacc
    24481 tggaattgca acataaggct ctcctgataa ataatttgct acgtctgcct ttagtggacc
    24541 ttctgttaaa cgtatttgac catttacagg tgttcccaca atatgaatat agctttttgc
    24601 cgatgtccca ttttcatagt ttgctgaaga gaaataacga tattttcttt tattggagtt
    24661 atctattcgc acttgcattc cttgtatttc tcttctttca ttacgtaccg gtatcaaaaa
    24721 acctgaatta ctataaaaat cccaatcatt atatttatca ctaaaaaaac ctgcaatccc
    24781 tttcaagtca tattttagtg atagtttcca gcatatcctt tttcttatat catctacttg
    24841 tggtatgctt ttatattggt tttcttctat agttttttta gataatccac gttgtagaag
    24901 gttagtttta tgtttgtcag acaatgtaag catatctaaa aattccgtgt aaactttatg
    24961 cctttcagat atactttttg cgttttgttc ttcatccaag tactgcctgc gttttgtttc
    25021 cttataggag atattgtctg atgtagattt gtatatcccg tctcctgact tcagcatctt
    25081 atatgcttcc tttgtgtcaa cttcttttaa gtaagctatt agatgaagta ttccaccatg
    25141 agtaccacat ttaggacatc tgaaaacgtt tataaatcta ttaagattaa gatggtattt
    25201 agtgtctcca ctaggacaaa aaggacagca agctgctact tcttttgttg tatgagtttc
    25261 gttttttatt cgtaatccta aatctctcgc tatatcaact atgtcatatt ctagcaaatc
    25321 aataccatgt ttacccatat attatctcct ctttgttatt attaactttt ctattccgtt
    25381 ctccttattt attaataaac ttcataaaag aaaaaagggc ttgtaatctg ctttgttatt
    25441 ttacaaaccc ttttttacta tttatttata aaatccagtc ttcttaaaac aatttctacc
    25501 atgtgagtgt ttcattaaat tctgtaagag ttccatcgaa ctctccattg aattcttcta
    25561 gtatatcttc gtctatatct tcgtccatta tttctcctga tgcatttgtt tcttttgtgt
    25621 ctaatgtatc tgtttctaag tctgaggata aatgttcttt tactactact acttctccat
    25681 tattattttc ttcctcattt tcatcctcaa atacgccacc tagtgatcgg gtcattttct
    25741 gcattccttt tgagtaggct agcatttctg cagcagtttc aggtgttaac ttttgtattg
    25801 ctgcaaaggt aacttggcta taagttgctt ttgataaatt acctacagct ctttttagac
    25861 ttattttggt aactacatct gtctttctta taggtgtagt tacaagtctt gtaatgtatt
    25921 tcctccaatt tgttattgac attgttggaa tatcaatata aattggaaat gtgtctcctt
    25981 ggcgcaggat gaatatacga tgcttatctg agcattcttt gcctacatct gggataaatc
    26041 tgtttttagg acaattggca catagtccac ctataatacc tattccttct atcccaccaa
    26101 cactagaaca atccggcttt acccctgcct gaacattttt gctcttataa taagttttta
    26161 tagggtaatg atctatcact acacctacta tctcagtgca tatttcggga ttttccccgg
    26221 actgtatttc aaagctgaga cctcctccgg aagggagttt aacacgctta aatgcactga
    26281 aacttatttg cccccccaac tcttcttcaa tgagttgagc cattagccgc cctttctcta
    26341 acgctactag atttttttcc atagtgatac ctccattttt tattttttat tgataaaata
    26401 taaaagagaa gtcaatatta ctgttatatt aaaacttctc ctttttagga aacgcagcgg
    26461 gaatcctcct catttgaatg aggcagatgg aagctgtg
//
LOCUS       DAOSDD010000027        26353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_849_length_26353_cov_5.143053, whole genome shotgun sequence.
ACCESSION   DAOSDD010000027 DAOSDD010000000
VERSION     DAOSDD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 26353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26353
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_849_length_26353_cov_5.143053"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK733_04175"
     CDS             <1..462
                     /locus_tag="PK733_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ09774.1"
                     /translation="VFTSMALKDNISSGESYYIAEIKSLKRIIDSLNPDIPCLCLIDE
                     VLRGTNTVERIAASSELLFYLSRNNCLPLAATHDLELGRILAKYYMNYHFQEYVTDND
                     IVFDYKIYPDISSTRNAIKLLKLMGYGDIIVDSSEERAKVFLEYGKWKEIS"
     gene            complement(633..2948)
                     /locus_tag="PK733_04180"
     CDS             complement(633..2948)
                     /locus_tag="PK733_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ09775.1"
                     /translation="MKRILALILSVLMITSLAIPAFASAEVKPAAEEARDMPAEVKEA
                     PAQVQATSEEVKATADKESGLEDAIKAVKRKFDIPDKAKFDNYSVYDHNGEKVWYLNW
                     YDNEQDIRVNVTVTSSGKITNYNYYKPYDYSERKFPKFGEEEALEKAKDFIRLVAPEG
                     TLAKIEKQDYFQNPLMNHYYYFNFYRSINGTPFYINNLSINVNSETGEVHSYYYNMDE
                     DLIFPDAKGSISLEDAEKAYAEKLGLKLIYQYSYDSKKKNVSIYPVYVPKYDNNMFAV
                     DAITGEKIQTTYPYYGVSFNESAQNNMKEKDALRMAAGDMGGVVLTPEEIKAAEEQSN
                     LLTEKNAEKIARETSEIGLDDNFQLRSWYLNRSWPMQNEFVYNLQFEKAIEKPEEKRN
                     EVLYVGISLNAKTGEIISFYTNNPYTEEKPKYDEDTARTAVEEFLKKFKEDKFDEVEF
                     QEPENYSEENPEFDNPEKEPQRYYYFNYIRKVNGIHFPGNSINVGFDAVNGKVTNFNM
                     NWFDVEFPGIENVLSLDEISKALLEKIGLELQYRRIDKRNAPIPLPKMEIMELDNKPE
                     IKLVYALKSGKPYVLDANTGDILDYDGKPFKEEKPVEYTDLSGHFSEGKVKELAKYNI
                     ITFKGPEFKPNDEIKQKDLFMILSKLLDRYYVPFITEDSSQDEIDEMYKQLVREGIVK
                     AGEKAPESAILREDAVKFLIRALKYDKIADVSEIFNVTFKDAQDITPELKGYVAIASG
                     LGIISGSGGNFNPKKTLTRGEAAVMIYNYLD"
     gene            complement(3105..4591)
                     /locus_tag="PK733_04185"
                     /pseudo
     CDS             complement(3105..4591)
                     /locus_tag="PK733_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
     gene            complement(4623..6023)
                     /locus_tag="PK733_04190"
     CDS             complement(4623..6023)
                     /locus_tag="PK733_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ09776.1"
                     /translation="MSSNSSNNNDNSIGNNREGNSGNNPGDNIRNNAENNPDIRYSYL
                     IDNLNNDEINIRLGSLNQLHQLIEAGEILKPERGNDVNNHIHTTYSFSPYSPTKAIWM
                     AYSAGLVTAGIMDHDSIGGAEEFIEAGKYLNIATTIGVECRADFSDTPLNGKKINNPD
                     QSSIAYVALHGIPHTRINHVREYFTPYTRERNKRNACMVDKINGLFKPFNIALDFEKD
                     VASISKVSDGGSITERHILFALAEKLVGRFGKGAALVDFLKNKMNISLKAKLEEYLLD
                     SENQFYEYDLLGVLKSDLISSIYMEAKKECPDIRELLEFANTIGAISAYAYLGDVTNS
                     VTGDKKTQKFEDDYIEQLFEVLKELGFNAVTYMPSRNTITQLKRVKALCNRYDLLQIS
                     GEDINSPRQSFVCKAMRNDEFSNLIDSTWALIGHEKAATENIDDAMFSAKTIQKYGDL
                     NERIAIYKEIGKSIGE"
     gene            complement(6217..7005)
                     /gene="srlD"
                     /locus_tag="PK733_04195"
     CDS             complement(6217..7005)
                     /gene="srlD"
                     /locus_tag="PK733_04195"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HOJ09777.1"
                     /translation="MVKTNRGRLEGQVAIVTGSAQGLGEALAKRLDKEGCRVVVADIN
                     LEKAQQVAECLTDAIAVKVDVSSEEQVENMIGKAIEKYGKLDLFVSNAAILIAKPTVE
                     FTAQEWKKMMEINLIGYFLCVKAAAKVMIPNKKGNIIQINSKSGKKGSYKNSAYAASK
                     FGGIGLTQSLALELAEYNIRVNAICPGNLLDSPLWVNSLYDQYAKNQGITVEEVRQKY
                     LNQVPLGRGCTYDDVANAMVFLASNEASYMTGQAINVTGGQEMR"
     gene            complement(7089..7856)
                     /locus_tag="PK733_04200"
     CDS             complement(7089..7856)
                     /locus_tag="PK733_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ09778.1"
                     /translation="MLNERQQQILNLMESGKGDIQLQELKPLFPDVSTMTLRRDLINL
                     ENEGYLIRTYGGAVSVKKVTNGVTEEDDYSIRATENVDAKMKIAHKAVTMVEKGRSIF
                     FDAGSTLMCLARIIPDDSFSILTSGANIALEIVKKLRPSVVTLGGQINRNTLATSGPY
                     ANSLLDILNVDLAFMSASGFSLESGFTVGNIYECELKRKVTKRAQKVILLMDTSKLNK
                     NLPFTYANLQDIDIWVCEKPLPSEIEAEAKKFGVQII"
     gene            complement(8048..10519)
                     /locus_tag="PK733_04205"
     CDS             complement(8048..10519)
                     /locus_tag="PK733_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ09779.1"
                     /translation="MPKSQYVDPMEIRKSGKITFKDIPVNQYNKTIDEEKANYTKEDF
                     LRIFRDMAIIREFETMINAIKTTSEYNGIHYSHPGPAHLSLGQEASAVGQAYLLDKDD
                     FIFGSHRSHGEVLAKGLSAIEKLSDSELMDIMENFFGGRALRIVEGIQKNKGNVKELA
                     VDFLVYGILAENFARETGFNKGLGGSMHLFFTPFGIYPNNAIVGGSADISVGAALFKK
                     INRKKGIVIANIGDGSMGCGPVWEAMCLAAMDQYKLLWEGEYKGGLPLIINFFNNQYG
                     MGGQTRGETMGYDMLARVGAGVNPEQMHSERVDGYNPLAVIDAIKRKKKILDEKNGPV
                     LLDTLTYRFTGHSPSDASSYRTKEEVEAWMEQDSLLTYRKELVDAKVANDDEFDAILD
                     SVRELITKTCKLAVDDNVSPRMDLVKDPEVIGKLMFSNQKIEKMDDRECEVLMPKDEN
                     PRVQQIKKKIRTAMEDGKPVSKNRVFQLRDAIFEAILDKFYVDPTLIAYGEENRDWGG
                     SFAVYRGLTEALPYHRLFNTPISEGAIVGSAVGYGMCGGRVIAELMYCDFLGRAGDEI
                     FNQLSKWQAMSAGILKMPIVVRVSVGSKYGAQHSQDWTSLTTHIPGLKVVFPVTPYDA
                     KGLMNSALAGTDPVIFFESQRIYDIGEFFHEGGVPEGYYEVPIGEPDIKKEGKDVTIL
                     TVGATLYRALDAAKILEEKYGLSAEVIDTRSLVPFNYEKVLESVRKTGKIVLASDACE
                     RGSHLKDIAQTLSEVAFDYLDAPPVVVGSRNWITPAHELEDYFFPQPEWIIDAIHEKI
                     MPLEGHVPANNFTVNEQLRRNRFGI"
     gene            complement(10733..12097)
                     /gene="lpdA"
                     /locus_tag="PK733_04210"
     CDS             complement(10733..12097)
                     /gene="lpdA"
                     /locus_tag="PK733_04210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOJ09780.1"
                     /translation="MTYDLIVIGGGPAGYLGAERAGQAGLNTLLIEKRSVGGVCLNEG
                     CIPSKTLLYSAKMYDNARYSEKYGVISENVTFNHGKVVDRKNKVVKTLVAGVKGQLKK
                     SNVTIVEGFGEIAGRSSEGYEVKVNNESYFGKRLLIATGSMPVIPPITGVKEGLEKGY
                     VLTNREILDLKEVPGSLVIVGGGVIGLEMASYFNSAGSKVTIIEMLDHIAGNNDRQIS
                     ELLMKNYEKKGIVFKLNAKVVEVKEGSVVYEYSGEKNAVEADKVLMSIGRRPVTQDTG
                     LDKIGVETERGRIKTDEMGQTNIPGVYAAGDVNGFSMLAHTAYREAEVCINNIIGKKD
                     IMRYNAIPYVIYTNPEVAGAGETEETAKQKGIDVEVANISLRYSGRYLAENEGGDGIC
                     KILVEKNSRRLVGVHMIGNYSSEIIYGAALMIEMEMRVDDIKEVVFPHPTVSEIIREG
                     IFSL"
     gene            complement(12299..13783)
                     /locus_tag="PK733_04215"
     CDS             complement(12299..13783)
                     /locus_tag="PK733_04215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOJ09781.1"
                     /translation="MAVPVIMPRQGQSVESCIISKWYKKKGDQVKEGDILFTYETDKA
                     TFDEEAKIEGTILEIFFEEEDDVPILTNVCVIGQEGEDFSEFDPREKKEDASANASET
                     DTSGAQTAGAGNGTAEGVKGTDNDKVSDYTGNTAVIAESKTAEGRIKISPRARNFAEK
                     SGIDYSYANSTGPDGRIIERDIITLKEQGPVFTLAAKREALRAGVNAVTEGTGIGGRI
                     TTGDLSRGTQAEAGTNAQTAASGATASGTTLAGATGIVGASAGDEPESIEVKLTNIRK
                     VIAKSMHLSLSSTAQLTLNASFDATEIMNYRKKIKENKDKLGLQNITLNDIVLYAVSR
                     TLINHKNLNAYFLDDKMKIFNIVNLGVAIDTERGLMVPTVFHADKKSLNEISGEVKKL
                     AEDCQKGTINPDLLTGGTFTVTNLGMMDIESFTPVLNPPQTGILGVNNIVQRAREVNG
                     QFEYYPAMGLSLTFDHRAVDGAPAAKFLKELKANLENFSVLLAK"
     gene            complement(13974..14855)
                     /gene="lipA"
                     /locus_tag="PK733_04220"
     CDS             complement(13974..14855)
                     /gene="lipA"
                     /locus_tag="PK733_04220"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOJ09782.1"
                     /translation="MQNNLNKPAFKPKPDWLRIKVQGDHKLAETEDILKRLSLNTVCE
                     EANCPNRMECFNKRTATFMILGRVCTRNCTFCNVEKNVPQPVDPDEPARVARAAAEMK
                     LKHVVITSVTRDDMPDGGAGHFARVIEEIRKLSDEVVIEVLIPDFKGNEEALLKVVEA
                     GPEIINHNVETVPALYSGVRPKAVYERSLALLANVKKANKNIYTKSGIMLGLGEKKNQ
                     VEEVLKNLRKVECDFLTIGQYLAPSKKHHPVIEYIHPDTFEEYKKLGYDLGFKYVASG
                     PLVRSSYHADEAMKALL"
     gene            complement(14959..15882)
                     /locus_tag="PK733_04225"
     CDS             complement(14959..15882)
                     /locus_tag="PK733_04225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ09783.1"
                     /translation="MNNIKPKRERTRTSSFGTSGRINHDSSSFYDSKLYNDFKVKSNI
                     NFEENQIPLENLNKIFLKSSEKMNEIPDSSVHLMVTSPPYNVKKDYDKDLSLDEYRNL
                     LRTVFKETYNKLVAGGRACINVANLGRKPYIPLHSYIIEDMLNIGFLMRGEIIWNKAS
                     SASPSTAWGSWLSASNPVLRDIHEYILVFSKDSFSRKQSKKINTITKEDFLEWTKSVW
                     TFPAVSAKSVGHPAPFPEELPYRLIQLYTYESDVVLDPFCGSGTTCLAALKSKRNYIG
                     YEVEPEYVELANKRIAPYTNQVSLFNLEGES"
     gene            complement(15890..16762)
                     /locus_tag="PK733_04230"
     CDS             complement(15890..16762)
                     /locus_tag="PK733_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09784.1"
                     /translation="MLDMLWNPKYEESFFRESSKFASKEQLFYVTDDRRYVAYWPKGY
                     DGTKSTLQSRNSLIGNFTEKWTSELIGRAIADKGLFAVQGAICDEIALPPLSPADVVI
                     STNRNIRQKTEEILAIFEVKMSIVWNWEMKPNGELICIGDYRTHQGNPGLLRSDSMLK
                     AIGKSINIRISSLKASKIPIIVIGNTPITNSYHTKVDHLKMSGIIQGFWSVNPAPCSI
                     KEKTLKGTVKEGYLRIDNYNELRKQIGNILNEGREYFSGMRSKEELGKIIERASEENT
                     YEKKAEYFLKMLRE"
     gene            complement(17062..17724)
                     /gene="lipB"
                     /locus_tag="PK733_04235"
     CDS             complement(17062..17724)
                     /gene="lipB"
                     /locus_tag="PK733_04235"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOJ09785.1"
                     /translation="MGKIRVARLGKMDYKEALSIQEKLLAERQAGEGEDTLLLVEHPS
                     VLTLGRRGKQNNIIVPEEYLKANNIKVYEISRGGDVTYHGPGQIVGYPIMDLHNYGKD
                     IKDFVWKIEETFIRLLKEEYGIEACREENKYTGVWVGEEKITAIGIAVKKWVTMHGFA
                     FNVNTNLDHFKWIIPCGLADRGVTSIQKVTGKHQDFERLNDLVVKYFCEVFDLEPELS
                     GI"
     gene            complement(17827..19092)
                     /locus_tag="PK733_04240"
     CDS             complement(17827..19092)
                     /locus_tag="PK733_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ09786.1"
                     /translation="MKTKAVRLYGKNDLRLDEFELPPIKDDEILARIVSDSICMSSYK
                     AAIQGADHKRVPKDVDKNPIIIGHEFCGEIIEVGKKWQSKFKAGNKFSIQPALNMESN
                     PYAAPGYSFQYIGGAATYIVIPNEVMEQKCLLNYDGEAYFYGSLAEPMSCIVGAFHAN
                     YHTTGGSYIHSMGIKEGGNMAILAGVGPMGLGAIDYAIHCDRKPGLIVVTDIDDNRLN
                     RAASIYTVEEAKKHGITLKYVNTSGLENVPEYLKSFTDGKGYDDVYVFAPVRPVVEQG
                     DKILGKDGCLNFFAGPTNPNFSAEFNFYNVHYNSTHIVGTSGGNTDDMIESLEMMEKG
                     LINPSAMVTHVGGLDSVAATTLNLPNIPGGKKLIYTNIKMELTAISEFREKGKSDPVF
                     VRLADIVAQNNGLWCTEAEKYLLENAKQI"
     gene            complement(19563..20846)
                     /locus_tag="PK733_04245"
     CDS             complement(19563..20846)
                     /locus_tag="PK733_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ09787.1"
                     /translation="MLNLMPIMGKLTKKFKIKSFSGLLPIVPIIAIVLAVILGFSLLF
                     FRFVMPFYSNKNPQNIENNFDRNNNSSNKLKDMKDLVKHKTLNLKINGLEQVINILET
                     NIGTNGIKVRPVLSHDSVYGFELLSEMAARYNAYAAINAGFFYEYGEPSGMVLIDGEI
                     ITGSTGKYPVLVIKDGEAELRELATELWLRSEAGEPESKTASLKLDSLNVEAKIGKWV
                     LYTRRYGTDNRVKKRNVTMVIEDNIIKKIIVSDKETEIPKNGMLATFIEPSATSATDN
                     PSNSSSLPQNSSEIAPFSEGEKVKFEYKPFLGSNGQAYECGSWIIKDGKIVIGENDPW
                     IGVLTNRDPRTAVGIKETGEVVLITVDGRQPGYSYGLTAQELGECLIQYGIRDAAMLD
                     GGASTEMIIEGKIVNKPSFRSSERPLAGAIIMEEQ"
     gene            complement(21030..21452)
                     /locus_tag="PK733_04250"
     CDS             complement(21030..21452)
                     /locus_tag="PK733_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOJ09788.1"
                     /translation="MDIRDISYIARSNSKFDIENNSVKNAGNRKENKIDDFSDNNSFE
                     AKLKRAMDQNDEKALKEVCQNFEEIFLQMMYKQMKATVPKSDLISEGAGREIFDSMLD
                     EALMEEASKGNGIGLADMMYKQLYKSMSNVYKKDIAEE"
     gene            complement(21514..22320)
                     /gene="flgG"
                     /locus_tag="PK733_04255"
     CDS             complement(21514..22320)
                     /gene="flgG"
                     /locus_tag="PK733_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOJ09789.1"
                     /translation="MMRSLWTAGSGMIAQQFNVDVISNNLANVNTTAYKKNRAEFKDL
                     LYQTMGRAYVLEDRGRPVNLQVGHGTVVSATVKNFETGSFEKTDNPFDFAIDGDGFFS
                     VLGPDGDILYTRDGSFKISMTDEGKMLATSDGYPVLDELGNNIVFDHEMDLSELNVSE
                     NGDLSYTDEDGIVMPLDQKIGIFNFGNRNGLISIGKNLYATTSASGETIPNEELANKS
                     ILIQRYLETSNVQVVEEMVRLITAQRAYEINSKAIQSSDEMLGMANNLKR"
     gene            complement(22504..23283)
                     /locus_tag="PK733_04260"
     CDS             complement(22504..23283)
                     /locus_tag="PK733_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOJ09790.1"
                     /translation="MIRGLYSSGWSMKANNRKMDVIANNLANVNTNGYKKDSVVFKSF
                     PELVTQRVNDNKDSSNPSGRIGRMELSSDVDEVFTYYYQGPTIRTDSKTDFSISDMGS
                     AFFTIAVMGEDGNIREYYTRDGAFIINDNNILVTKEGYPVMGQNGSINIYDNNFIVEE
                     DGTIIQNGDITDRFLIRNFSDTSTLRKIGSNLIERTADTQEQPFNGAVKQGFLEQSNV
                     NVIKEMVDMITVMRAYEANQKILQIQDGTLEKAVNEVGSVR"
     gene            complement(23411..24622)
                     /locus_tag="PK733_04265"
     CDS             complement(23411..24622)
                     /locus_tag="PK733_04265"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOJ09791.1"
                     /translation="MSLLDISRENVTRVLSLSTQELAGLSFECSCGKKHSVDIKKIII
                     EKGAINKVPEVAVDFKKGKLFLVADKNTYSVCGKHVDELLKEDGFNLKTFVFNTPHQL
                     SPDASTLGRLLIEIELGTSLIIAVGSGVINDMCRYLAYKLGIPFIIVGTAPSMDGYAS
                     VVTPTIVDGIKATLGGIYPYAIIGDIDILKDAPMEMLYAGFGDILGKLNALADWDLAR
                     QLHGEEYCEICASFVKNALTKCIDNIDGIRNREDEAIKYLMEALVIAGVSMGMFGNSR
                     PASGAEHHFAHYWDEDAIARGEEHPLHGNSVGVGTVVAAEQYKLIKDRLPVKVNVPEP
                     EEVTALLKRLGAWYNPADLGISRELFKRSILHSKDSRPKFTSSHFAGEIGALDEIADK
                     LADKFYEEIVK"
     gene            complement(24736..25092)
                     /locus_tag="PK733_04270"
     CDS             complement(24736..25092)
                     /locus_tag="PK733_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOJ09792.1"
                     /translation="MEKINITCIVCPKGCNLEVYLADGKVTEVKGHSCKRGLDYGWSE
                     STNPRRVLTTTVRIDGARLAIIPVKSEKPLPKGLLFDCIKAINRVRIKAPVNIGDVVV
                     ENILGTGVNIVTTRSM"
     gene            complement(25254..26216)
                     /locus_tag="PK733_04275"
     CDS             complement(25254..26216)
                     /locus_tag="PK733_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ09793.1"
                     /translation="MIYIENTDKIAAYPNKDTSLLKNKKLFILDMDGTFYLGDRILEG
                     SLDFINKLKETGREFLFFTNNASKTPEFYIKKLQKMGCEIIKKNIATAGDVTIEYLLE
                     NYNGKKVYLVGTPLLEESFRKAGINLVLDKSENKFRQGSEERSGEGSGERSEEKSGEG
                     SEEKSNGKTDEKLDEKPDVVVVSFDLTLTYEKISRACTFIREGAVFIATHMDLNCPTE
                     DGFIPDCGSICAMVTASTGVKPRYLGKPFEETIEMIEHITGYTKDEMVIIGDRLYTDI
                     ATGFNNGVASILVLTGETKLEDLEESQIKPDYVFPSLSSVIQWI"
BASE COUNT         7199 a         5852 c         3959 g         9343 t
ORIGIN      
        1 gtttttacct ccatggcatt gaaggataat ataagcagtg gtgaaagcta ttatattgct
       61 gagatcaagt cgcttaaacg gataattgac agccttaacc ctgatattcc atgcttatgc
      121 cttattgacg aggtattaag ggggactaat actgttgaac gtatagctgc atcctctgag
      181 ctgttatttt acctgagcag gaacaattgc ctgccccttg ctgctactca tgatcttgaa
      241 ctgggacgta ttctcgctaa gtattacatg aattatcact ttcaagaata cgttacagat
      301 aatgatattg tttttgatta caaaatttat ccggacatat ccagcaccag aaatgcaata
      361 aaacttctaa agctaatggg ctatggagat ataatcgtag actcgtctga agaacgtgcc
      421 aaagtgtttt tggagtacgg gaagtggaaa gaaatttcat aacttatttg acttaaaaaa
      481 gagccgcttc cgtcttcgag aagcagctct ttttatatag tctattgttg tcttttgttg
      541 ccttttgttg tcttttgtat tgaaattggg ttttaacagt taaaaatttt gattccaaaa
      601 ctattaatta ctttctctga ctactgtgcc aattagtcaa gataattata aatcatcaca
      661 gcagcttctc ctcttgtgag ggtcttctta ggattgaagt ttccgccgga accagaaatt
      721 attccaaggc ccgatgcaat tgctacatat cctttgagtt ctggagtaat atcttgtgca
      781 tctttgaatg taacattaaa tatttctgaa acatctgcga ttttgtcata cttcaaggcc
      841 cttataagga attttacagc atcttccctt aatatagctg attcaggtgc cttttcaccg
      901 gctttcacta ttccttctcg caccaattgt ttatacattt catcaatttc gtcctgactg
      961 ctgtcttcag taataaaagg tacatagtat ctgtcaagca attttgataa aatcatgaag
     1021 aggtcttttt gctttatttc atcattgggc ttgaattcag gccctttaaa ggtaattata
     1081 ttgtatttcg ccagttcttt aacttttcct tctgaaaaat gtccgctcaa gtcggtatat
     1141 tcaacaggtt tttcttcctt gaagggtttt ccatcgtaat caaggatatc cccggtgttt
     1201 gcatctaata catatggttt tcccgatttc agtgcatata ccagtttgat ttctggttta
     1261 ttgtctaatt ccataatttc catttttgga agtgggatag gggcattcct cttatctatt
     1321 ctcctgtatt gaagttcaag tcctattttt tcaaggagag ccttgcttat ctcatcaaga
     1381 gatagtacat tttcaattcc agggaactca acatcaaacc agttcatatt gaagtttgtc
     1441 actttgccgt ttactgcatc aaaccctaca tttatactat ttcctgggaa atgtatacca
     1501 tttacttttc tgatatagtt gaaatagtag tatctctggg gttctttttc aggattgtca
     1561 aactcaggat tttcttcaga atagttttct ggttcctgga attcaacttc atcaaattta
     1621 tcttccttga acttctttaa aaattcctca acagcggttc tggctgtatc ttcatcatat
     1681 tttggttttt cctctgtgta tggattgttt gtatagaagc taattatttc tccggtttta
     1741 gcattaagag atattcctac atataaaact tcgttccttt tctcttccgg tttttctatt
     1801 gctttctcaa actgcagatt atatacaaat tcattctgca tgggccagct tctgttaaga
     1861 taccagcttc taagctgaaa gttatcgtcc aagcctattt cggatgtttc tctggcgatt
     1921 ttttctgcat ttttttcagt tagaagattt gactgttctt ctgcagcttt gatttcctca
     1981 ggtgtaagca caactccgcc catatctcct gcagccatcc ttagtgcatc tttttctttc
     2041 atgttatttt gagctgattc attaaaacta acaccatagt aggggtatgt agtctgtatt
     2101 ttctctcctg taattgcatc aacagcaaac atattgttgt catatttagg aacatacact
     2161 ggatatatgc ttacattctt tttctttgaa tcataactat actggtatat aagtttaagc
     2221 cccagttttt ccgcatatgc cttttcagca tcttcaagag atatagaacc ttttgcatcc
     2281 gggaagatca aatcttcatc catattatag taataactgt gaacttcacc tgtttcactg
     2341 tttacgttaa tgctgagatt attaatgtaa aacggtgtgc cgtttattga cctataaaaa
     2401 ttgaagtaat aataatggtt cattagagga ttctgaaaat aatcctgttt ttctattttt
     2461 gcaagagttc cctcgggagc aaccagtctt atgaaatcct ttgctttttc aagggcttcc
     2521 tcttcaccga attttggaaa tttgcgttct gaatagtcat acggcttgta atagttataa
     2581 ttagttattt ttccgctgct ggttacggta acattgaccc ttatatcctg ctcattatca
     2641 taccagttta aataccatac cttttctcca ttatggtcat acacactata gttatcaaat
     2701 ttagccttat caggaatatc aaactttctc ttcacagcct taatggcatc ttccagtccg
     2761 ctttccttat ctgctgttgc cttgacttct tctgaggttg cttgaacttg tgccggtgct
     2821 tctttaactt ctgctggcat atccctggct tcttctgctg caggcttaac ctctgctgaa
     2881 gcgaaagctg ggattgccag tgaggtaatc atcaatactg ataaaattag tgctaatatt
     2941 cttttcataa ataccaccct tcaaattatg tattttagca ttttatgcat catgataatt
     3001 tgacgggtgg cactttgtaa aagttccggc agttggtatt gtttaccttt ttttaatcaa
     3061 ctcgtaatat agatgtaatt taatactact ataccaaatt atttttattg attgcttctc
     3121 atcccaagta tttgtttgta tctttcgtat gcttccttcc atgcatcagt attagaaggg
     3181 gtatagacag tagtcgggaa ggattcagcc accagttcac gggcttcctt taaccccgaa
     3241 acctccttca atgcatcagc tggcacatga gatttcctac ggatgtcgct tctacaggtc
     3301 ctgtaataac attcttttca gctgcatttg cagtaaacct gcacagcatc agattcctgg
     3361 atccgccccc aactatatga agtacaggta tgttatatcc tgcgattttc tcaagtccct
     3421 taatggtcat gttatatttc agagcaaggc tctccattat gcatcttact atagatgcct
     3481 tatcttcagg cactttctgc cctgtttccc tgcagaattt cactattttt tcaggcatgt
     3541 ttccaggtct gtaaaaaata tcatcatcag ggttaataaa gcatttaaat gcctccgctt
     3601 tttctgccat ttcctccagc tcatcatagc cgtaagattc gccggctttg tcccaggccc
     3661 ttttacattc ctggaatatc caaagtccca taatattctt caacagtcta gtggttctgt
     3721 tatatccacc ttcgttggta taatccaagt caaaggtagt ctcatttatt attggttcgg
     3781 ataactcaac tcccagaagc gaccaagttc cactgcttaa atatgcaaac ttaccttcct
     3841 gtgcagggac agatacaact gcgcttgcag tatcatggcc ggcgacagca ataacaggta
     3901 tactgttttt tagagtaagt tcgtctttaa tttctttatt tgtaccgcca attacagtgc
     3961 ccgggtcaat aatatctgca agtatgtttt gaggtatgcc catagcactt aaaagtctgt
     4021 cagaccacct gcctgttctg ggattaagca tttgagtcgt gctggctatg ctgtattcgc
     4081 tgaacttttc gctcgtgaga aaataattaa ataaatccgg cataagaagc atcttctttg
     4141 ctttttcaag tagaggggaa ttattaagct tcattgatag aagctgataa agagtgttta
     4201 gttccataaa ctggatgccg gtttcttcat atatttccct ttttggtata atattaaagg
     4261 cagcctccaa cataccctga gttctcacat ccctgtaatg tacaggattg cccagaagtt
     4321 ctccggatgg acctattaac ccaaaatcca caccccaggt atcaactcct attcccgaaa
     4381 tattattatg tccttcctga acacatttaa gaattccttt cttcatttca tggaataatc
     4441 ttaaaatgtc ccagtaaaat gtaccgttta acattacagg gtcatttgaa aatctgtgaa
     4501 tttcttttaa agatagtttt tttccgtcat atgtacctaa aataccacga ccgctgcttg
     4561 caccgtaatc aaatgccaga agttcaagca tatttaacca ctccctgtat aataatttta
     4621 tactactcac ctatggattt gccaatttct ttatatattg ctatcctctc gttcaaatca
     4681 ccatacttct gtatagtttt tgcagaaaac atagcatcat caatgttttc tgtagcagct
     4741 ttctcatgtc ctataagagc ccatgtagaa tctataagat tgctgaattc atcattcctc
     4801 atagccttgc atacaaaaga ctggcgtggg gaattgatat cctctccgct tatctggagt
     4861 aggtcatatc tattacaaag tgcctttact cgcttaagct gtgtaatagt atttctcgag
     4921 ggcatatagg taacagcatt aaacccaagt tccttcaaaa cctcaaataa ctgttcaatg
     4981 taatcatctt caaacttttg tgttttttta tcacctgtta ccgagttggt tacatctccg
     5041 aggtatgcat aggcagatat agcacctata gtattggcaa attcaagtaa ttctcttata
     5101 tccgggcact cctttttagc ttccatataa attgaactaa ttaaatcgct ttttaatact
     5161 cccagaaggt cgtattcata aaattggttt tctgaatcaa gaaggtattc ctccagcttt
     5221 gctttaagac taatattcat tttgtttttt aggaaatcca ccaatgcagc tccctttcca
     5281 aaccggccca ccagtttttc cgcgagagca aaaagaatat gtctttctgt gatactaccg
     5341 ccatcactta cttttgaaat agaagccaca tctttctcaa aatcaagtgc aatattaaaa
     5401 ggtttgaaaa ggccatttat tttatccacc atgcacgcat tacgcttgtt tctctctctt
     5461 gtatagggtg taaaatactc tctaacatgg tttattcttg tgtgtggtat accatgaaga
     5521 gctacataag ctatggacga ttgatcagga ttattaatct ttttgccgtt aagcggagta
     5581 tctgaaaaat cagctctgca ctccacccct atagtagttg ctatattcaa atacttgcct
     5641 gcttctataa attcctcggc cccgcctatt gaatcatgat ccattatacc tgcagtaaca
     5701 agtcctgcac tatacgccat ccatattgcc ttagtgggtg aatatgggga aaaggaatat
     5761 gtagtatgaa tatgattatt aacatcgttt cctctttcag gctttaatat ctcaccggct
     5821 tcaattagtt gatgcaactg atttaacgag cccagtctta tattaatctc atcattgttt
     5881 aaattatcaa taaggtagga atatcgaatg tcagggttat tttcagcatt attcctaatg
     5941 ttatctccag ggttattccc agagttacct tcgcggttat taccgatgct attatcattg
     6001 ttattactgg aattgctact catataatac ggctccttat ttattttagc tgtcaccgca
     6061 cgattctgta taaatatgta gagttttcgg gactaaacta tctttttaat ctttatcttt
     6121 ctaatcttta tctttctaat ctttatcgtt ctaatcttta tcgttctatt ttattaaata
     6181 atcaattata tcattaatta ttcagtaatt attcagttat ctcatttcct gtccacctgt
     6241 aacatttatg gcctgacctg tcatatagct tgcttcattc gatgccagaa acaccatagc
     6301 attggccaca tcatcatagg tacagcctct tccaagaggt acctggttca aatatttttg
     6361 tcttacttct tccacagtta ttccctggtt ttttgcatat tggtcataca ggctgtttac
     6421 ccataaaggt gaatccagca aatttcccgg gcaaatggca tttaccctta tattatactc
     6481 tgcaagctcc agggcaaggc tttgagttag tcctattccg ccaaactttg atgctgcata
     6541 tgcagagttt ttataagaac ccttcttccc ggatttgctg tttatctgga ttatgttgcc
     6601 ttttttgttg ggaatcatta cttttgcggc tgctttgaca cacaaaaagt atcctataag
     6661 atttatttcc atcatttttt tccattcctg tgctgtaaat tctacagtgg gctttgctat
     6721 cagaatagct gcattggata caaacaagtc taacttgcca tacttctcta ttgccttacc
     6781 tatcatgttt tcaacctgtt cttcgctgct tacatcaact tttactgcta ttgcatccgt
     6841 taaacactct gcaacctgct gggctttttc cagatttata tcagccacaa ctaccctgca
     6901 gccttcttta tccagccttt tggccagcgc ttctccaagt ccctgtgccg accctgtaac
     6961 gatagctacc tggccttcta atcttccccg gtttgttttt accatatcca ccccaccttt
     7021 atactagcta aatattatat atttatataa agcacacaaa agaagctgta ttatcaaatt
     7081 ttgtatgctt atattatttg taccccgaat tttttcgctt cagcctcaat ttcagagggt
     7141 aacggcttct cgcacaccca tatatcaata tcctgaagat ttgcataggt aaatggcaaa
     7201 tttttattca gtttacttgt atccatcaac aatattacct tctgtgcacg tttagtaact
     7261 tttcttttca attcacattc atatatattt ccaactgtaa aaccactctc caaggaaaaa
     7321 cctgatgccg acataaatgc caggtccaca ttaaggatat caaggagaga attggcatag
     7381 gggcctgatg tagcaagagt atttctatta atctggcctc caagagtaac aacagatggt
     7441 ctcagtttct taacaatctc aagagctata tttgctccac tggttaatat agaaaagcta
     7501 tcatccggaa ttattcttgc cagacacatt aaagtacttc ccgcatcaaa aaatattgac
     7561 cgccccttct caaccatggt tactgcctta tgtgctattt tcatctttgc atccacattc
     7621 tctgtagccc tgatggaata atcatcttct tcggttacac cgtttgtaac tttcttaacg
     7681 cttacagccc ctccataagt acgtatcaga taaccctcat tttcaagatt gataaggtct
     7741 cttcttaatg tcatagttga cacatcggga aataacggtt tcagttcctg aagctgaata
     7801 tctcccttcc cggattccat taaatttaat atttgctgct gtctctcatt tagcacataa
     7861 tcacttccaa accaaatcac tttcaaacta aatttatttc agaactaact gtttttgaac
     7921 taactgctta tgtatcaaag aaccatcacc tatgcagata ttacctgtac aagtggtggt
     7981 ccattgataa tataacctaa aaggaatatc tcctgctaga gagaggcgta tccccttaca
     8041 ttacctttta aatcccaaat ctatttcttc tcagttgttc attaactgta aagttattag
     8101 ccggaacatg cccctctaaa ggcataatct tttcatgaat tgcatctata atccattccg
     8161 gttgagggaa gaagtagtct tccagttcat gagcaggtgt tatccagttt cttgaaccta
     8221 caacaactgg tggtgcatcc agataatcaa atgcaacttc gctcaaagtc tgagctatat
     8281 ccttcaaatg tgatcctctc tcacaagcat cacttgccaa tactatttta cctgttttcc
     8341 taacagattc caatactttt tcataattaa atggtacaag ggaccttgtg tcaataactt
     8401 cagctgaaag cccgtacttt tcttcaagaa tttttgcagc atcgagagct ctgtaaagag
     8461 tagcgccaac ggtaaggata gtaacatctt ttccttcttt ctttatatct ggctccccta
     8521 ttggaacttc ataataacct tcaggtactc ctccctcatg gaagaattct ccaatgtcat
     8581 atattctctg actctcaaag aatataacag gatctgtgcc tgcaagagca ctattcataa
     8641 gaccttttgc atcataaggt gttacaggga atactacttt taaccctggt atgtgagtag
     8701 ttaatgatgt ccagtcctga gaatgctgag ctccatattt ggaaccaaca gatactctta
     8761 caactatagg catcttaagt atacccgcac tcattgcctg ccactttgaa agctggttga
     8821 atatttcgtc tcctgctctt ccaaggaagt cgcagtacat taattcagca ataactcttc
     8881 cgccgcacat tccataccca actgctgaac caactatagc gccttctgaa ataggagtat
     8941 tgaataatct atgatatggc agagcttcag tcaggccgcg gtatacagcg aaagatcctc
     9001 cccagtctct gttttcttca ccatatgcta tcaatgtagg atctacatag aatttatcaa
     9061 gtatagcttc aaaaattgca tctcttaatt ggaataccct gtttttggat acaggcttcc
     9121 catcttccat agctgttctt attttctttt taatctgctg tactctcggg ttttcatcct
     9181 ttggcatcaa tacttcacat tctctgtcat ccatcttctc aatcttctgg ttggagaaca
     9241 ttaactttcc aataacctca ggatctttta caaggtccat cctcggagat acgttatcat
     9301 cgacagcaag cttgcatgtt ttagttatca attctctaac actgtcgaga atagcatcaa
     9361 attcgtcatc atttgctact tttgcatcaa caagttcttt cctgtaagtt aagagggaat
     9421 cctgctccat ccatgcttct acttcttcct tggttctgta actggatgcg tctgaaggtg
     9481 aatgccctgt gaacctgtag gttaatgtat caagaagcac aggtccgttt ttctcatcaa
     9541 gtattttctt ctttctcttt atagcatcaa taactgctaa cgggttgtac ccgtcaactc
     9601 tttcagaatg catctgttca gggttaacac ctgctcctac gcgggcaagc atgtcatatc
     9661 ccattgtttc tccgcgagtc tgtccaccca taccgtattg gttattgaag aagtttataa
     9721 tgagcggaag tccgccttta tattcgcctt cccataataa tttatattgg tccatagcag
     9781 ccaggcacat agcttcccat acaggtccgc atcccattga gccatcaccg atatttgcaa
     9841 taacaattcc ttttttccta tttattttct taaataatgc cgcaccaaca gaaatgtctg
     9901 ctgagccgcc tacaatagca ttattaggat aaattccaaa aggtgtaaaa aacaggtgca
     9961 ttgaccctcc taagccttta ttgaaacctg tttcacgggc aaagttttca gccagtatac
    10021 catacaccaa aaagtctact gccagttctt taacattacc tttatttttt tgtataccct
    10081 caacaattct cagagctctg ccgccaaaaa agttttccat tatgtccatt aattcgctgt
    10141 cacttagttt ttcaatagct gaaaggccct tggcaagaac ttccccatgg cttctgtgag
    10201 aaccgaaaat gaaatcatct ttatcaagca gatatgcctg ccctactgct gatgcttcct
    10261 gccccaatga aaggtgtgca ggaccaggat ggctataatg aataccatta tattcgcttg
    10321 tagtcttgat ggcatttatc atggtttcaa attctctgat tattgccata tccctgaata
    10381 ttcttaaaaa atcttccttt gtatagtttg ctttttcctc atcaattgtt ttgttgtatt
    10441 gatttacagg aatatcttta aaagtgattt taccgctttt cctgatttcc attggatcaa
    10501 catattgtga tttaggcatt tatttcccct cttttcaaat tattatacat ttttaatgtg
    10561 cattagattt tttgctttta tctgaagctt ctatcttcca acctctagca tcctgcatcc
    10621 agcattacaa ttctcatttt gatctttcta ctttccactt tacaaccaac tagtcaacta
    10681 gccaaccaac taaccaacta actaaccaac tagccaacta accaaccaac tcctataagc
    10741 tgaaaatacc ttcccttatt atttctgata ctgttggatg cgggaataca acctctttaa
    10801 tatcatccac tctcatttcc atctcaatca ttaatgcagc tccatatatg atttctgatg
    10861 agtaattgcc aatcatatga actccaacaa gtctgcggct gttcttctcc acgagaatct
    10921 tgcatattcc gtctccgcct tcattttccg caaggtatct tccactgtat ctcagggata
    10981 tatttgctac ctctacatca atccccttct gttttgctgt ttcttcagtc tcgcctgcac
    11041 ctgctacttc aggattggtg tagatgacat aaggtattgc attatatctc attatgtcct
    11101 ttttaccaat aatattgttt atgcaaacct cagcttccct gtaagctgtg tgagccagca
    11161 ttgagaaacc atttacatca ccggctgcat aaactccggg tatattggtc tgccccattt
    11221 cgtcagtctt gatacgtcct ctttcagtct caactccgat tttatcaaga cctgtatcct
    11281 gggttaccgg tctccttcca atactcataa gtaccttatc ggcttcaact gcattcttct
    11341 cgccgctata ttcataaact acagagcctt cctttacttc tacaactttt gcatttagct
    11401 taaatacaat accttttttc tcatagtttt tcataagaag ctcggatatt tgtctgtcgt
    11461 tgttcccggc aatgtggtca agcatttcaa ttattgttac cttgcttcct gccgaattaa
    11521 aataggatgc catctcaagc cctattactc cgcctcccac aattacaaga gaccctggaa
    11581 cttcttttaa atccagtatt tctctatttg tcagaacata gcctttctcc agaccctctt
    11641 taactccggt gatcggaggt attaccggca tggatcctgt agcaataaga agccttttac
    11701 caaagtaact ttcattatta actttgactt catacccctc actgcttctc cctgctattt
    11761 ctccaaaacc ttcaactatg gtaacattgc tttttttaag ctggccttta acacctgcaa
    11821 caagtgtttt aacaacttta tttttcctgt ctactacctt gccatgatta aatgttacat
    11881 tttcagatat aacgccgtat ttttcgctat atctggcatt atcatacatt ttagctgaat
    11941 acagcaaggt ttttgaggga atacatcctt cattgaggca cactccgccc actgatcttt
    12001 tttctattaa aagagtatta agtcctgcct gtccggctct ttcggcccca agatagcctg
    12061 caggtcctcc tccaataaca attaagtcat atgtcatatt tctcaactcc attaacttat
    12121 ttcgcaattc aaattaattt gtttcataac tctatcatta attcaattca taattcaatt
    12181 cataatttaa ttcataattc aattcataaa ttaatcaact taattcctgg ttcaattaac
    12241 ctgtttttaa caatagttaa caaatataat tcataattat aattaaaatt aataaaattt
    12301 attttgccaa aagaacactg aaattctcca aattagcttt caattccttg aggaacttgg
    12361 ctgcaggagc tccatctact gccctgtgat caaaggtaag tgaaagtccc attgccggat
    12421 aatattcaaa ttgaccgtta acttctctcg ccctttgaac aatattgtta acaccaagta
    12481 tgcctgtttg cgggggattc aataccggtg taaaagattc aatatccatc attcccagat
    12541 ttgtaactgt aaatgttccg cctgttagaa gatcagggtt tatggtgcct ttttgacaat
    12601 cttcagcaag ctttttaact tctcccgata tttcattgag ggatttttta tctgcatgga
    12661 atactgttgg aaccatcagt cctctttcgg tatcaattgc aactcccagg tttacgatat
    12721 taaatatctt cattttatcg tcaaggaaat atgcattaag attcttatga ttaattaatg
    12781 ttcttgatac tgcataaaga actatatcat taagggttat attctggaga ccaagcttat
    12841 ctttgttctc ttttattttc tttctatagt tcattatctc tgttgcatca aaagaagcgt
    12901 ttaaagttaa ctgcgcagtt gatgaaagtg agagatgcat tgactttgca attacttttc
    12961 ttatgtttgt aagttttact tctattgatt caggttcatc tccggcagat gctcccacaa
    13021 ttccagttgc tcctgctaac gttgttccgg acgctgttgc accagatgct gctgtttgag
    13081 cattggttcc tgcttcagcc tgtgttcctc tgctcaaatc tcctgtagtt atccttccac
    13141 ctattcctgt accttccgtt actgcattca ctcctgctct tagagcctct ctctttgcgg
    13201 caagtgtaaa aacaggtcct tgttctttga gagtaataat atctctttca attattctac
    13261 catcaggtcc tgtagagttt gcataactat agtctatacc cgacttttct gcaaagtttc
    13321 tggctctggg tgatatttta atccttcctt cagcagtctt gctttcagca attactgcag
    13381 tgtttcctgt ataatcggaa accttatcat tgtcagtgcc tttaacacct tcagcagtcc
    13441 cgtttccagc accggctgtt tgagcaccgc ttgtgtcagt ctctgaagcg tttgctgatg
    13501 catcttcttt cttctcacga gggtcaaact cagagaagtc ctctccttcc tggcctataa
    13561 cacatacatt ggtcaaaatc ggcacatcat cttcctcctc gaaaaatatc tcaaggatag
    13621 tgccttctat cttggcttcc tcatcaaaag tggccttatc cgtttcatat gtaaacagga
    13681 tatcgccttc ttttacctgg tcgcccttct ttttgtacca tttggatata atacaacttt
    13741 caacagattg cccctgtctg ggcataatta ctggtacagc catctatttc tctccttttc
    13801 aataagatta ccttttcccc aatattcttt tgcacttttg tatatatttt ttataccctt
    13861 tgtattcttt ttatatacct tgtgccatat ctaccttcat aatgttattt tataacactt
    13921 attagttata ttttatcacc ggatatgtca tatgtcaaca tgatatagga gtgttataac
    13981 aatgccttca ttgcttcatc agcatgatat gaactcctta caagaggtcc tgaagctaca
    14041 tatttaaatc ccagatcata ccctagtttt ttatattcct caaaagtatc aggatgtata
    14101 tactcaatta caggatgatg ttttttagat ggtgcaagat actggcctat tgtaaggaaa
    14161 tcacattcaa ctttccttaa gttcttaagt acctcttcca cttgattttt tttctctcct
    14221 aatcccagca taatacctga tttggtgtaa atattcttat tggctttttt aacatttgca
    14281 agcagggcca gcgatctttc atatacagcc ttgggtctta ctccggaata aagagccggt
    14341 accgtttcaa cattatggtt tatgatttca ggcccagctt ctaccacttt taaaagagcc
    14401 tcttcattgc ccttgaaatc gggtataagt acttctataa caacttcgtc actcagcttc
    14461 cttatttctt ctattaccct ggcgaaatga cctgcaccgc catcgggcat atcgtctctg
    14521 gtaacagacg ttataacaac atgcttaagt ttcatttctg ccgctgcccg agccactctg
    14581 gctggttcat ctggatctac aggctgcggc acattctttt ctacattgca gaatgtacaa
    14641 ttacgagtgc atactctgcc taaaatcata aaagtagcgg ttcttttatt aaaacactcc
    14701 atcctgttcg gacagttagc ctcttcgcaa actgtattaa gggacaatcg ctttaagata
    14761 tcttcagttt ctgccagctt gtgatcaccc tgcaccttta ttctaagcca gtcaggttta
    14821 ggcttaaagg ccggcttgtt taagttgttt tgcatattaa ccacctttcc gtcactgtgc
    14881 aatggaatta ctgtaatcaa ataatgcata tcctattagc acatgagtat atgttcttgt
    14941 aatacatatt cgtaatattt aggattcccc ctccaaatta aataagctta cttgatttgt
    15001 atatggagct attcttttat tagctaactc tacatactca ggctcaactt cataccctat
    15061 ataatttctt tttgatttaa gcgctgccag gcaggttgtc ccactcccac aaaaaggatc
    15121 aagtacaaca tccgattcgt aagtatataa ttggatcaat cgataaggta gttcctcagg
    15181 gaaaggagcc ggatgtccta cactttttgc agatacagca ggaaaagtcc atacactttt
    15241 tgtccattca agaaaatctt ccttagtaat agtatttatt tttttagatt gttttcgtga
    15301 gaatgaatcc tttgagaaaa ccaatatata ttcatgaata tccctaagga caggattaga
    15361 agccgataac cagcttcccc atgctgtcga aggactagca cttgatgctt tattccaaat
    15421 gatctcgccc ctcatcagga atccaatatt tagcatatct tcaattatat agctatgtaa
    15481 aggtatatat ggttttcttc ctaagtttgc tacatttata catgctctgc cgcctgcaac
    15541 caacttattg taggtctctt taaatactgt ccttaataaa ttcctatatt cgtccaatga
    15601 tagatctttg tcataatcct tttttacatt ataaggaggt gatgtaacca ttaaatgaac
    15661 actagaatca ggtatttcat tcattttttc actggatttc aaaaatattt tatttaaatt
    15721 ttctagcgga atctggtttt cttcaaaatt aatattactc tttactttaa aatcattata
    15781 taatttgcta tcataaaagc ttgaagagtc atggttaatc cttccactgg taccaaatga
    15841 gcttgttctt gttctttccc gctttggttt gatgttattc actcaatatt cactccctta
    15901 gcatttttag aaaatattct gctttttttt catatgtatt ttcttcactt gctctttcaa
    15961 taatctttcc taattcttcc ttactcctca taccagaaaa atactctctt ccctcattca
    16021 aaatattacc tatctgtttt cttaattcat tataattatc aattcttaaa taaccctctt
    16081 taactgtgcc tttcaaagtc ttttccttaa tactgcatgg tgccggatta accgaccaaa
    16141 acccttggat aatgccggac attttcaaat gatcaacttt ggtatgataa ctatttgtta
    16201 ttggtgtatt acctattaca ataatcggaa ttttagatgc ctttagactt gatattcgta
    16261 tgttaatgct tttaccaata gctttaagca tcgaatctga tcttaacaac cccggattcc
    16321 cctgatgtgt tctataatcg ccaatacaaa taagttctcc gttaggtttc atttcccaat
    16381 tccatacaat tgacattttt acctcaaata tagctaaaat ttcttccgtc ttttgcctta
    16441 tatttctatt tgtagaaatg actacatctg caggggataa aggtggtaaa gctatttcat
    16501 cacaaatagc accctgtact gcaaataatc ctttatcagc aattgctctt ccaattagct
    16561 ccgaagtcca cttttcagta aagttaccaa ttaatgaatt cctactttgg agagtacttt
    16621 ttgtaccgtc atatcctttc ggccaatatg caacatatct acgatcatcg gttacataaa
    16681 acaattgctc tttggaagca aatttacttg attctctgaa aaagctttct tcatatttag
    16741 gattccaaag catatccaac attacacacc tcattatctg tcgtttatta tattacatat
    16801 tattacataa tttcttcttt atatcaagtg ttttggacca agcaattcaa gacattttca
    16861 acctttaact atactttatt attatcactt tattataggt ttactgaaat atacagaata
    16921 tataatataa atattgttcc ttaatatacc acatcgcaag attatttgtt tgcagccata
    16981 cgcttccata tgagattaag atattactaa ttaatatata ataatatagc attattcctg
    17041 tttacttccg ccagtttcag ttcaaatgcc tgatagctcc ggttccaggt caaacacctc
    17101 gcagaaatat ttgactacca ggtcattcag cctttcaaaa tcctggtgtt tacctgtaac
    17161 tttttgaatg gaagttaccc ctctgtctgc caggccacat ggtattatcc atttaaagtg
    17221 gtcaaggttt gtgtttacat taaaagcaaa tccgtgcata gtcacccatt tctttacggc
    17281 tatgcctatt gcagtaatct tttcttcacc aacccagaca ccggtatatt tattctcttc
    17341 cctgcaggcc tctataccat attcttcctt aagaagtctt atgaaagttt cttctatttt
    17401 ccagacaaaa tccttaatat ccttcccata attatgaaga tccatgatag gatatcctac
    17461 tatctggcct ggtccatgat aggtaacatc tccgcccctg cttatttcgt atacttttat
    17521 attattagcc ttcagatatt cttccggtac aataatgtta ttttgcttgc ctcttcttcc
    17581 taaagtcaac acagaagggt gttccaccag caatagtgta tcttctcctt ctccggcctg
    17641 tctttcagct aaaagctttt cctgaatgga aagtgcttcc ttataatcca tttttcccaa
    17701 tctggctact ctaatttttc ccatcttttt tgtctctcca catttttatg cccataccaa
    17761 atattatagt tatttggtat gggctattat atacttttta cttttcacac tgtttctatg
    17821 ctattcttat atctgtttag cgttttctaa gagatatttt tcagcctcag tgcaccataa
    17881 accattgttt tgcgcaacaa tatcagccag ccttacaaaa acaggatctg atttcccttt
    17941 ttctctgaac tccgaaatag ccgtaagctc catttttatg tttgtataaa taagcttttt
    18001 gccacccgga atattaggca ggtttaatgt tgtagctgca acactatcca gtccgccaac
    18061 atgggtaacc atagctgaag ggttgatcaa ccctttttcc atcatttcaa gggattcaat
    18121 catatcatcg gtattcccgc cgctggtccc tacaatatgg gttgagttgt aatgtacatt
    18181 gtagaagttg aactctgctg aaaagttagg attcgtaggt cctgcaaaga agttcagaca
    18241 cccatccttg ccgagaattt tatctccctg ctcaactaca ggtcttacag gtgcaaatac
    18301 atacacatcg tcataacctt tgccatctgt aaaagattta agatactcgg gtacattttc
    18361 caaacctgaa gtgttgacat attttagagt aattccatgt ttcttagctt cctctacagt
    18421 atatatggaa gcagccctat ttagcctgtt atcgtcaata tcagttacta caataagtcc
    18481 gggttttctg tcacagtgaa tagcataatc aatagcaccc agtcccattg gccctactcc
    18541 tgcaagaata gccatattgc cgccttcttt tatacccatg ctgtggatat aggaaccacc
    18601 tgttgtgtgg taattggcgt ggaatgctcc tactatacaa gacataggtt cagcaagaga
    18661 accatagaaa taagcttctc catcataatt aaggaggcat ttttgctcca tgacctcatt
    18721 aggaataaca atatatgtag cggcaccgcc aatatactga aatgaatacc ctggagctgc
    18781 atatgggtta ctttccatat ttagtgcagg ctgtatggaa aacttgttgc cggctttgaa
    18841 cttgctctgc cactttttgc cgacctctat aatttcacca caaaactcat gaccgattat
    18901 aattggattc ttatcaacat cctttggaac tctcttatga tcagcaccct gaatagctgc
    18961 tttataagag gacatacata tgctgtctga aacaatcctc gcaagaatct catcatcttt
    19021 tattgggggc aattcaaact catccaacct taaatcattt ttgccatata atcttactgc
    19081 ttttgttttc atattaagct ccattcctgc agcgtgcgaa atgaattcac ccttgcaaga
    19141 acaaaacttt tttatgaaaa agtttttatg ggcttaaatt caatttagtc gcacgtgcaa
    19201 tttatttttt aaaattttat acaaaaaata tgcacatgtg cattagtttt tcagaaactt
    19261 atcaatttct ttcaaactaa ccacctcata tttatttttt aaacctgtta cacatttatt
    19321 aactggtaca tttattttaa ctgaaaacac ctattaacgt atatttacct gttaaccggt
    19381 atatttattt gctaactggt atatttgcca acaatatcct tcaccatata tatttacatg
    19441 ttatatcata acataaaaat gatagatttg aacatagttt tttaaaattt ttgaaattta
    19501 tttgtacgct ccataattct cctcaataac atttttttaa gtaaaattat taacctctgt
    19561 aattattgct cttccataat tattgcccct gcaagaggtc tttcagagct tctaaaggat
    19621 ggtttgttta ctatttttcc ctcaattatc atttcggttg acgcaccgcc atcaagcata
    19681 gcagcatctc taatcccata ttggattaaa cattccccca attcttgggc tgtgagacca
    19741 taactatatc ccggttgtct tccatcaact gttatcagca caacttcacc agtttccttt
    19801 atccccactg cagtccttgg atccctgttt gttaatacgc ctatccaagg gtcattttca
    19861 ccaatgacaa tctttccgtc tttaattatc cagctgccgc attcataagc ctggccattt
    19921 gacccaagga atggtttata ttcaaatttt accttttcgc cttcactaaa tggcgctatt
    19981 tcagaactat tttggggtaa actagatgaa ttagatggat tatctgttgc tgaagttgcg
    20041 gaaggttcaa taaaagtagc cagcatgcca tttttaggga tctcagtttc tttatctgac
    20101 acaattattt tttttataat gttatcctca attaccatag taacatttct cttttttacc
    20161 ctgttatcag taccgtacct gcgggtatac agcacccact tccctatttt cgcctccaca
    20221 ttgaggctgt ccagttttaa agaagccgtt ttagattccg gctcccccgc ttcagatctc
    20281 agccacaact ccgtagcaag ctcccttaac tcagcttctc catcttttat aacaagtacc
    20341 gggtatttgc ctgtagaacc cgttataatt tctccatcaa ttaaaaccat gcctgatggc
    20401 tcaccatatt cataaaaaaa gcctgcatta attgcggcat atgcattata cctagctgcc
    20461 atttcagata acaattcaaa tccatataca ctatcatgag atagtaccgg tctgactttt
    20521 attccatttg taccaatgtt tgtttccaaa atattgatga cctgttctaa cccatttatt
    20581 tttaaattaa gtgttttgtg ctttacaagg tctttcatat ctttcaattt attggagctg
    20641 ttattattcc tatcaaagtt gttttcaatg ttttgagggt tcttattact atagaaaggc
    20701 ataacaaacc tgaaaaacag caatgaaaac ccaaggatta ctgctaacac aatagcaatg
    20761 atcggtacaa tcggcaacaa cccactgaat gactttattt taaacttttt tgttaatttt
    20821 cccataatag gcattaagtt tagcatctat tttccctaaa tttgctttct actggaattt
    20881 actctttaca gtatattata ccctttttaa atcaaataag cagtccttat ttgactgctg
    20941 aagtgaatat atacatagta attaatattt gtcatatttt cctccaaata tagaaaatgc
    21001 acccttggtt gcctatactt aatgcctgct cactcttccg caatatcttt cttataaaca
    21061 ttgctcatac ttttgtaaag ctgcttatac atcatatctg ccaacccaat accgttacct
    21121 ttagaagctt cttccatcaa tgcctcatca agcatagaat caaatatttc ccttccggct
    21181 ccttctgaaa ttaagtcaga ttttgggact gtggctttca tttgtttgta catcatttgc
    21241 aggaaaattt cctcgaaatt ctggcacact tctttaagag ctttttcatc attctgatcc
    21301 atggcacgtt ttagttttgc ctcaaagcta ttattatcag aaaaatcgtc aattttattc
    21361 tcttttctgt tcccggcatt tttaacactg ttattttcaa tatcaaattt gctgttactt
    21421 cttgcaatat aacttatatc tcttatatcc atacttttac ctcatatcat gtattccaca
    21481 ctacatcatg tcatccatct tacaccaatg tatttatctt ttcaagttgt tagccattcc
    21541 cagcatttca tcagaagatt gtatagcctt tgagttaatc tcatacgctc tttgagctgt
    21601 aattaatctt accatttctt ctaccacctg cacattggat gtttctagat acctttgaat
    21661 caatatgctt ttgtttgcaa gttcctcatt aggtattgtt tctcctgatg cagatgttgt
    21721 agcataaaga ttttttccaa tgcttatcaa tccatttctg ttcccaaaat taaagatccc
    21781 tattttctga tcgagaggca taacaatccc atcttcatcg gtatagctta aatcaccatt
    21841 ttcagaaaca tttaattcag acaagtccat ctcatggtca aatactatat tatttcccaa
    21901 ttcatcaagg acaggataac cgtcagatgt agcaagcatc ttcccctcgt ctgtcatact
    21961 gattttaaag cttccatccc tggtatacaa aatatctccg tcaggtccaa gcacactaaa
    22021 gaagccatca ccatcaatag caaaatcaaa aggattgtct gttttttcaa agctgcctgt
    22081 ctcaaagttt tttactgtag cagagaccac ggttccatgc ccaacctgca aatttacggg
    22141 cctgcccctg tcttcaagaa catatgccct gcccatggtt tggtaaagaa gatccttaaa
    22201 ttctgcccgg ttctttttat aagcagttgt attaacattt gcaagattat tggatattac
    22261 atcaacatta aattgctggg caatcattcc tgagcccgca gtccaaaggg atctcatcat
    22321 aaaaatacag cctccccgat attttttcaa gtatgatttc agtgcaaaaa ctcattcggc
    22381 ggtcaacata ttgtaatttg taacacttca ccttgcattt ttacgggcac acgctgattc
    22441 aacatcgtgt ttcagcagcg ctttccacgc catcaatggt gcttcaaatc ttccaaatat
    22501 agtttacctt acacttccca cttcatttac tgccttttcc agggttccgt cctgtatctg
    22561 caaaattttc tggtttgctt catacgcacg cataactgtt atcatatcaa ccatttcctt
    22621 aataacattt acattggatt gctccaaaaa accctgcttt acagcaccat taaagggttg
    22681 ttcctgagta tctgccgtcc gttcaattaa gttgctgcct attttccgca gtgtggatgt
    22741 atcagagaaa ttcctaatta ggaacctatc ggttatgtca ccattctgaa ttatagtccc
    22801 atcttcctct acaataaagt tattatcata aatatttata gaaccgttct ggcccataac
    22861 cggatatcct tcttttgtaa caagtatgtt gttgtcgtta attataaatg cgccatctct
    22921 tgtgtagtat tctcttatgt ttccgtcttc ccccataacg gcaatggtga aaaaagcact
    22981 tcccatatca ctgattgaaa aatctgtttt gctatctgtc cgaattgtag ggccctggta
    23041 gtaataggta aatacttcat caacatcact gctaagctcc attcttccta tcctgccaga
    23101 gggatttgag ctgtctttat tatcatttac acgttgtgta acaagttcag gaaagctctt
    23161 aaaaactacc gaatcctttt tataaccatt tgtattgaca ttcgccagat tgtttgcaat
    23221 tacatccatt tttctattat tggccttcat gctccagcct gaagaatata accctctaat
    23281 catataattc cctttagtcc cttccagtgt tttaaacaca gcgacaataa gtgccgatat
    23341 attatatatc ggcacttatt gaagatagtt tatatattct aaaataatta ataaatttct
    23401 ttaatagttt ttatttaact atttcttcat aaaacttatc tgcaagtttg tctgctatct
    23461 catcaagcgc gccaatttca cctgcaaagt gggagcttgt aaacttaggt cttgaatctt
    23521 ttgaatgtaa aatactacgt ttgaaaagct ctctgcttat tccaaggtca gcgggattat
    23581 accaggctcc aagtcttttt aaaagagctg ttacttcttc cggttccggt acatttactt
    23641 ttacaggaag cctgtctttt attaatttat attgttctgc tgcaacaact gtccctacac
    23701 caacagagtt cccatgcaga ggatgctctt ctccccttgc aattgcatct tcatcccaat
    23761 aatgagcaaa gtgatgttca gctccggaag caggtctgga gtttccgaac atgcccatgg
    23821 atacccctgc tataaccaat gcttccatta aatattttat agcctcatct tctctattcc
    23881 taattccatc aatattatct atacattttg taagggcatt ttttacaaaa gacgcacaaa
    23941 tttcacagta ttcctcgcca tgtaattgtc ttgcaaggtc ccagtctgca agagcattaa
    24001 gtttccccaa aatatctcca aaccctgcat aaagcatctc catgggagca tctttcaata
    24061 tatctatatc tccaattata gcataaggat atattcctcc caaagtagct ttgataccat
    24121 ctactatagt aggtgttacc actgaagcgt aaccatccat agaaggagct gtgcctacaa
    24181 taataaaggg aattcccagt ttgtatgcaa gatatctgca catatcgttt ataactcctg
    24241 aacctacggc tattattaag gatgtgccta attcaatttc aataagaagc ctgcccagag
    24301 ttgacgcatc cggggaaagt tgatgagggg tattaaaaac aaacgttttt aaattaaacc
    24361 catcttcttt taataattca tctacatgtt taccgcatac actatatgta tttttgtcag
    24421 ctaccaggaa cagcttaccc tttttaaaat caacagctac ctccgggact ttgttgattg
    24481 cccctttctc aattattatc tttttaatat ctactgagtg cttcttcccg cagctgcatt
    24541 caaaagataa tccggccagc tcttgtgtac tgagactcaa tacccttgtt acgttttccc
    24601 ttgaaatgtc gagtagactc atatttttct cctcctttat acatattata ccatattccg
    24661 ccatgttctg ttgtgttttg tcatgttctg ttgtgtttct gtcatgttct acctatcata
    24721 tccctttcat aatccttaca tacttcttgt agtaacaata tttacaccag tacctaaaat
    24781 attttcaact actacatctc ctatgtttac aggagctttt attctaactc tattaattgc
    24841 ttttatgcag tcaaaaagca ggcctttcgg caggggtttt tcagatttta ccggaataat
    24901 cgcaagtctt gctccatcta ttctaacagt cgttgtaagc actcttctgg gattggtact
    24961 ttccgaccag ccataatcca gtccccgttt gcaggaatgg ccttttacct cagtcacttt
    25021 tccatctgct aaatatacct caagattaca ccctttcggg cagacaatgc aagtaatatt
    25081 tattttttcc ataacggtag cgtcttcctt tcaacataac attcgcttaa caatagcatt
    25141 catttttaca ataacattcg ctttatacaa caacatttat taagtccatc tgcacttaat
    25201 ccattgaatt gcgctgtaca taaatctcat aaatcgattg aattacgtcg ttcctaaatc
    25261 cactgaatta cactgctcaa agaaggaaat acataatccg gtttaatctg tgattcctca
    25321 agatcttcca gctttgtctc tcctgtcagc actagaatgc ttgcaactcc attgttaaaa
    25381 cctgttgcta tatcggtata aagcctgtca ccgattatta ccatctcatc ctttgtataa
    25441 cctgttatat gttctatcat ttcaatagtt tcctcaaaag gttttccaag gtatctcggc
    25501 ttaacacctg tggatgcggt taccatggca catattgacc cgcaatcagg tataaagcca
    25561 tcctccgtag gacagttaag atccatgtgg gttgcaataa atacagctcc ttcacgaata
    25621 aaagtgcaag ccctggatat tttctcgtag gttaaggtta aatcaaaact aactaccaca
    25681 acatcgggct tttcatcaag cttctcatcg gtctttccat tagacttttc ttcagaccct
    25741 tctccagact tttcttcaga cctttctcca gatccttctc cagacctttc ttcagatcct
    25801 tgtctaaatt tgttttcaga tttatccaat acaagattta tgcctgcctt tctgaaactt
    25861 tcctcaagaa gtggtgtacc taccagataa accttcttcc cattatagtt ttcaagaaga
    25921 tattcaatgg ttacatcacc tgctgtggca atattcttct tgattatctc acatcccatt
    25981 ttctgcagct tttttatata aaattctggt gtctttgatg cattgttcgt gaagaagagg
    26041 aattcccttc ctgtttcctt aagtttatta ataaaatcca gagagccctc cagaatcctg
    26101 tctccgaggt aaaatgttcc atccatatca aggataaaaa gttttttatt ttttaacaaa
    26161 gaagtgtctt tattcggata tgctgcaatt ttatctgtat tttcaatata aatcattatc
    26221 ttacatacct ccacaactta atatctaaaa caatgcgagt atgaattatt acctgataac
    26281 ctgttaaatc ttagccttta ttatatcatg aatttcgaag aaattgcatg atataatcgt
    26341 gcatgtgcgt ttt
//
LOCUS       DAOSDD010000028        26181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_855_length_26181_cov_5.239838, whole genome shotgun sequence.
ACCESSION   DAOSDD010000028 DAOSDD010000000
VERSION     DAOSDD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 26181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26181
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_26181_cov_5.239838"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PK733_04280"
     CDS             complement(<1..1268)
                     /locus_tag="PK733_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09794.1"
                     /translation="MKKTKKISLVLIISILISMFTLPDISLVTVKAEDDSEMYYEKGY
                     FPVFYDENNKNTFESINSTLPPGTPSTITVNGSVFPFNYRLWFAKNIVVYGDYSTANS
                     RSNDFKAETDSPYGVGFYSKNGVRGEYRYHGFDVAGNRYNNTWWTIDVNTGSSINDNV
                     WLYRPWSKDTYNLEKISPYNEAAEWPRNQEDQKTKAWINRPIQKDGITLTFENAENNS
                     EREAYRYINIMSAPTINYPGEARAWYKYNNQLLYRTFTIDKLDHVDKLMTDVATSVKI
                     LTPENRLAIKDFGSGSSGNTLEYLDSEIEVEVEVSGVLLDEEYYKDPVKKTARYTRED
                     IKDWTISLGSDIRGSVKSVDYNKGKAIFILKYKVASILAQNTYTLKGNTRVNFKNDKS
                     NENTGSTNLVFKIENKIPSVLIKDKQMTAV"
     gene            complement(1320..2585)
                     /locus_tag="PK733_04285"
     CDS             complement(1320..2585)
                     /locus_tag="PK733_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ09795.1"
                     /translation="MMRRFLMVRMAFMLFTSTVAIALLTSVIFLSGSITVLAEMPTSG
                     SGFSTVLERLSAIENPDIEEAAKRFNDIKDHWSKEYIGKLALLEIVAGYGGSRFGPED
                     TLKVDEFLKMTLRAMGHKVEEGMEYWAEPYIKLAREEKIIEEKEFTDYRRPILREEAA
                     RIMVKTALKIEEAPIPNHTSYAKLRIPDYHDIGDEYKQHVLYAYSMGFIAGIGNGSFL
                     PKKTLTRGEGSAIVMRYLDEGMRKPMKPKDSEIVKFYDDYENKIYEIYPPSKTEVIDV
                     IRVMSDSAKKSKGLPLRGYYAPDEVVGTRLYKSKEAYEESSIFKDCSFSIRMAEWELN
                     GYDITIFDAKATKELHRDVFIDLLNHLLEDEAERAIKDFDHILELAIKRQGIPYQKEY
                     SLNNRIVIITKHNSDDGFGMYIRIKNKGN"
     gene            complement(2677..4395)
                     /locus_tag="PK733_04290"
     CDS             complement(2677..4395)
                     /locus_tag="PK733_04290"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HOJ09796.1"
                     /translation="MMVDIQNYGIRPIYERDDFSSYLVIKPEVNKLLNYQVEMIINNR
                     IPGLLPMDLRRKNNETCLYYNITSQMPLSQFLSRKKINKEEFVNLLSQITGIMLSGVN
                     YLLYNKCYILRVDYIYINPANFEINMVYIPAIMDEEVNQILRKFVINLIVNTAYIEDE
                     NTGDNFIQKIINYLKGETFNINGFNRLLMQLSTGGNQDEYTNTEDQMSIKSAPDTMGM
                     DGVSDIGSANSEAALEEKTGKVNSWDEVRCGKGDRGKGTEIDDKGKQCSYNTNDEEQG
                     GLLGNLILNHSKISGKVWMAIGSQLIILILILLSKKFFESLNGNIKTTYAAVALIIIA
                     IDTLLFKNLFGMGKTVDKPQSSPEGMCFTSGTQNISSKNINADNMNTQHTNIREDMNT
                     QHTNAWQDMNAQSNARQGINSEEQPYRGLNLSGFGKPEKQITREPISKEPMKSNSYFC
                     HEAKTALDYGGKTVLLSGKKEQKPMIINMKGNLQEEVPVNKADFLIGRLENQVDYTIK
                     NDTIGKVHAQIITRENRYYLKDLNSRNGTFINDIRIDCNTENEIKDGDRITFADMDYL
                     FVVRYI"
     gene            complement(4442..4657)
                     /locus_tag="PK733_04295"
     CDS             complement(4442..4657)
                     /locus_tag="PK733_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HOJ09797.1"
                     /translation="MTELMKRFMKDEDGVGTVEIVVIIAVLVGIALIFRDAIIKFAVK
                     VMKSVFGDDSIINDIKSNGIRNGNAIN"
     gene            complement(4794..5654)
                     /locus_tag="PK733_04300"
     CDS             complement(4794..5654)
                     /locus_tag="PK733_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ09798.1"
                     /translation="MSLAILAYLTAILVLYILSKGKYDNLVESLDKKQFPLKNLLPIG
                     LYIMDAIGCRYVSNYDRRLLVKISEIYGSKNSWHYLKIHWANKMVLFALGVFVILLIG
                     LGTAIDMTYAVLSICFLAMIVILSDRELDSKLKRRHLSIQWDFPDFLNKLTLLVNAGL
                     TTSRAWEKVIRENKKQSDLYREAEIVIAEINSGKSEIKAYEDFSRRCRTVEVARFVSI
                     VIQNIRKGNSELASIFRVLSNECWEMRKNTAKKLGEEASTKMVIPMMFMLVAILLIVS
                     TPAVLSIFKM"
     gene            complement(5669..6469)
                     /locus_tag="PK733_04305"
     CDS             complement(5669..6469)
                     /locus_tag="PK733_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadB"
                     /protein_id="HOJ09799.1"
                     /translation="MKKGAEKCNNKNSCREDNNIIDYSIYIMPLKEKIYHILKVMPII
                     YFLVYLFYKSHLFSLILSLAAFAWPSIENKKVIKKRRNELNLQFKDMLYSLHSSLSAG
                     KSVELAFVGAVDDLAILYPSPDTYIIMEAIYIVRKLEMNETIESALDDFAYRSGIEDV
                     QSFAEVLRICKRTGGNIADAIKRTTSIINDRIEIKQDIDTMLARKNFERKVLNIMPVI
                     MLVLLSATAGDYINPVYTVPIGRIVMSIAALLITISYFISKKIMDIDV"
     gene            complement(6490..7752)
                     /locus_tag="PK733_04310"
     CDS             complement(6490..7752)
                     /locus_tag="PK733_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOJ09800.1"
                     /translation="MVFDSSGDIYSNSDILCRIRNTVSSIISRNIDCGKDISDSEIRE
                     LITNAVFDISGEFYFSINEKHEIVETVFNSMRRLGAIQPFIDDESVTEIMVNGPGNIF
                     IEKGGKIYKSDVRFESTEKLEDVIQSVVSKANRVVNEASPIVDARLKDGSRVNVVLKP
                     IAVNGPVMTIRKFPQKPLTMEQLIKWNSITIEAADFLGNLVKARYNIFVCGGTGSGKT
                     TFLNALSNFISGDERIITIEDSAELQIKGIQNLISLETRNGNMEGRGKIGIRELIRTS
                     LRMRPERIIVGEVRGEEALDMLQAMNTGHEGSLSTGHGNSSKDMLSRLETMVLSAAPL
                     PLTAIRQQIASAIDVIVHLARMRDKTRKTVEITEVSGFKNGEIVLNPLFVFSDEGDTE
                     DGKVKGSLVKTENKMVKTAKFHTAGMHY"
     gene            complement(7742..8899)
                     /locus_tag="PK733_04315"
     CDS             complement(7742..8899)
                     /locus_tag="PK733_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ09801.1"
                     /translation="MRRLKLILADKDEPYIESLTGYLMEKHPYKFQVNSFTNKDHFKR
                     FLAEDEGIIDIVLVGEEFCDNLNGLEQDNKLPVIIRISERMTVSEYFSKEIAGCKNEI
                     YKYCSGEKLVKDISNIYEKEKYSNMLLEKEVKKTRMVSVYSPAGGTGKTTIAANLSIQ
                     YAREGMKVFYLNLESIRSVNCFPGSLPEKEAQNSFSKILFSLKEKDTDINHKIEAARC
                     KALQYDIYYFPPPDNAMELDEISACDIKYLVDQIKLMAQYDIIIADLASSFNQLNTAV
                     MEDCDEIVLVTTTDMVALSKMEHFVNSLQIYCRKKNISFIDKATIIINKWEEGSSFDI
                     NRLNSLLSTPGKNIDIKLPFEERLELQEWKADEINSFNRELRKLIGRFDGV"
     gene            complement(9458..9634)
                     /locus_tag="PK733_04320"
     CDS             complement(9458..9634)
                     /locus_tag="PK733_04320"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HOJ09802.1"
                     /translation="MQYGEKVTQLKEEIMMLQKKLGDSINDEKINSGNLLKISKELDA
                     LIVDYYNAKSDSEL"
     gene            complement(9994..11901)
                     /locus_tag="PK733_04325"
     CDS             complement(9994..11901)
                     /locus_tag="PK733_04325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOJ09803.1"
                     /translation="MKLKMTEAIINKKTKIKENKIVARVLENEYSYWIYLFISIMCKC
                     LYFQFTTQINRQPYFSHDNLAMIFSSFCILIIITGLIVMLFNRKRLTAIFVLNLIISS
                     ILTGDTNFFRYYYGILSIPVILQIDIKLFSSIQQSIESLFKVKDIIYILDIPMLLIWL
                     IKFRKKGIEKVNPANRLIAGVLSLALGVAGFISIYSKTDMDILIYNSNYITQKMGILY
                     SHFDGLKLYAKESFNKNKGLTQNESYSIKQYFGNKQEPGRKYRGIAKDKNLIVIQVEA
                     LQEFMINRKVNGVEITPNLNKLINESLYFNNFYYQVAGGNTSDAEFLLNTSLYPAKEG
                     AVYVRFAENTYHSLPEIMNNKGYNTYALHAFTAKFWNRTEMYKTLGFKEFIDGSYYEM
                     DDFAGWDGEALSDASFFNQSLDKIDTSRPFYSFFITLSSHYPFTFFEDYNFEAGELEG
                     AFIGNYIKAINYADSCLGKFIEELKQKGLYNNSLLVVYGDHSGVPKHLSGELMDFLGM
                     DYSDTQWIKLQKVPLIIHYPEQINGDTISVTGGQIDILPTIANLMDFKAPYAMGKDLL
                     NTKNGYVVLRNGTVITENYIYINERGEIYSAKDGKPLKIRDYQNNVKSFLKQLNISDL
                     IIEKNALEYIN"
     gene            complement(12315..13931)
                     /locus_tag="PK733_04330"
     CDS             complement(12315..13931)
                     /locus_tag="PK733_04330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HOJ09804.1"
                     /translation="MVHLNKENIKNKKLWENTSVNIPEFDLDKMISKTTEDPIWVHFG
                     AGNIFRGFIAALQQELLESGLAEKGIIAAETYDFEIIDRIYDPYDNMGILVVLYPDGS
                     FSKKVIASISESLVGDPSRGEDWARLKKVFTNPSLQMASFTITEKGYNINQLSGEFLA
                     DVKKDMEVGPENPIHVMSKVASLAYTRYKNGQLPIAFVSMDNCAENGKRLSTSVNTIA
                     RVWAEKGLVEKEFIDYLNDSKKVSFPWSMIDKITPRPAEEVKNVLDNAGIGGMDIIRT
                     NKNTFIAPFINAEGPQYLVIEDSFPNGRPPLEKAGVIFTDRNTVDRVEKMKVGTCLNP
                     LHTALAIYGCLLDYKTIAQEMQDDLLRKLVEKIGLEEGMPVVINPGVIDPVAFAKEVI
                     EVRLPNPYIPDTPQRIATDTSQKLKIRFGQTIAAYKNSPDLDAASLKYIPLVLAGWCR
                     YLMGINDAGKEMTLSSDPMLETLKPYFADIKLGNPESAAGKLKPILPNTELFGVDLYE
                     VGLGEKVEQYFTEMIAGENAVRNTLRKYLG"
     gene            complement(14092..15480)
                     /gene="pepV"
                     /locus_tag="PK733_04335"
     CDS             complement(14092..15480)
                     /gene="pepV"
                     /locus_tag="PK733_04335"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HOJ09805.1"
                     /translation="MIIGKEIQNHRNDIVKSLQELVQIKSVKDEACEGKPFGQGVGKA
                     LEYMLNLGESLGLKVKNVDGYAGHVEYGEGDEIVGVLVHLDVVPEGTGWSFPPFGGEI
                     HEGKIYGRGTSDDKGPAVVALYSLKALKDAGIVPKRRIRIILGTNEESGMSDMEYYFS
                     KEPVPHMGFSPDANYPIINREKGILHLVFEKGENESLHNNPIYTLRGGEVINMVPPEC
                     RAFIPEDKIKPGEVELLKDIAQKFNTSVSHINDINGKGIEILVKGKPAHAASPQNGIN
                     AIYRMLSFLNMAGRERYLDGFLKFLHEKIGEETTGSSLGIALKDEESGELTLNLGKII
                     KDDTGTKAFIDIRYPVTCKMEEVLAAIKEKTEKEGIVVKVLNHSEPLYVPETHPLIIR
                     LITAYEKITGEEAKLLSMGGGTYARTLKNNGVAFGGAGTGAHQPDENVSIDELMHHAE
                     ICTQAIYELACS"
     gene            complement(15663..17519)
                     /locus_tag="PK733_04340"
     CDS             complement(15663..17519)
                     /locus_tag="PK733_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOJ09806.1"
                     /translation="MKLNYDKKSKDPTYFIQVGFRNGKKTSTRNVMRIGKHSELLAID
                     PDPLAYAKRKVMEANEAQKHNKLSMSFEIDFDEKLTATSDIASKSNLLNTGYIILQKI
                     YHDLAIADFFKAVQTSHKATFSCNDINRFLTYARILDPESKLGTFDKLDTYYEQPSFG
                     YQHILRFMDILEKNYDEYIEHLFKNSGNIVPRDTSVCYFDCTNYYFEIEAEDPDCIDE
                     VTGEILKGFRKYGPSKQHQPLPLAQMGLFMDGNGIPISMCINSGSDNEQKCAIPLEKK
                     ITKMFGGERFIYCADAGLGSFSIRQYNSMGGRAFIVTQSIKKLSKTLKQAVFNDYDYK
                     LLSDDTPVTIKHMKDFDRFDKENLPLYNDFAYKVLDVENAVDLGLYEEIVLKNGKTKK
                     VKSKAILKQRIIITFSRKVMEYQRRIRNAQIERARNILKYRDVEEVKKGPHDVSRFIK
                     RTSVTMDGEKAVDHYEIDQAVIDEEEKYDGYYAVATNLYDDAKDILNINSQRYKIEDC
                     FRILKTNFCARPIHHSKENRIIAHFMICYTALLIYRLLECKLDQYGTHFTTDNILDTL
                     KNMNVSNTQDMYYTAAYKGSQVCTALNGLYGLGLDKKYYQPKELNKKIKKIL"
     gene            complement(17677..17844)
                     /locus_tag="PK733_04345"
     CDS             complement(17677..17844)
                     /locus_tag="PK733_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09807.1"
                     /translation="MIIGKEIQNHRNDIVKSLQELVQIKSVKDEACEGKPFGQGVGKA
                     LEYMRVFDTSN"
     gene            18013..18927
                     /locus_tag="PK733_04350"
     CDS             18013..18927
                     /locus_tag="PK733_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOJ09808.1"
                     /translation="MKHLFIINPVAGKGKALEYIPKIKDFFSRRQDDMYFIETTEKPG
                     HAVDLVKQYVSKDTYRVYSVGGDGTLNEVLNGIAGSDSSLAVIPAGSGNDFVKSLVNY
                     SPLYLSQKDEAKSVLLEKLAHGQEKLADIGRINDRYFINISSIGFDAEVVYNTNRIKK
                     LPLVTGIFAYILGVFSTLISYSKNILRISIDNNSIETDTLLVAVANGRYYGGGMQPAP
                     AASIYDGIFDICLIKGVGRLRILKFFPKFIKGRHSDVKEVSFHRSKQVKIYSNHKVAL
                     NVDGEVSMVDGEIDFEIIPGGIKVVIPG"
     gene            complement(19032..20825)
                     /locus_tag="PK733_04355"
     CDS             complement(19032..20825)
                     /locus_tag="PK733_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09809.1"
                     /translation="MARNKYDIDERLESEFNFNHLKRISRYVRPYRRKMALTLLLMII
                     TTSASLLGPYLIKEALDTKIPEKDVAGLVVLSAFFTLNLAIIAVCSRFRIELINDVGQ
                     SIMRDIRLDLFSHLQELPFSYYDSRPHGKIYVRVINYVNSIGNLLSNGLIQVIIDLIS
                     LVFIIVIMFSINARLMLLSLTGLPVLLLTVFLIKNMQRKAWQDVSAKQSNMNAYIHES
                     ISGIKVTQAYVRESVNLGILEDLGNKYRDSWMKAVNSSFILGPVVDNISTIVTALIYM
                     AGVSWIAGGVSLGVLIAFTSYTGRFWGPINNLSNFYNQIVTVMAYLERVFETMDEKPT
                     VADLPGAYEMPSITGKIEFKNVDFSYEEGGRRILNNVSFTANPGESIALVGPTGAGKT
                     TVVNLISRFYNINSGEILIDGINIQDVTLKSLRKQMGIMLQDTFIFSGNIIDNIKYGK
                     LDADDEEVIEAAKAVMAHDFIVEMNGGYYAEVSERGSTLSAGQRQLISFARTLLADPK
                     ILILDEATSSIDTKTEIALQQGLEKLLQGRTSFIIAHRLSTIKNASRIMYIDNGEIVE
                     QGTHDELIAKRGAYWELYTEQYKHLAGNEAG"
     gene            complement(20856..22601)
                     /locus_tag="PK733_04360"
     CDS             complement(20856..22601)
                     /locus_tag="PK733_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09810.1"
                     /translation="MRVFSFFWGYLKKYRFSIAAVFLIMMANALLGMVAPYISGIIVD
                     DVIKGGRNELLLKLLGILILGVALKSVARYFSLMLVEKTSQNFLFDVRNDLYSRIQNM
                     DFSYFDRTKTGNIMTRISGDLQVIRQFVAGSATVVFESLFSFVFAMIIMVRMNFLFTL
                     LLLSVTPFIGFYTFKMTQKTKPLNSEWHEQVTRLNSVVQENISGNRVVKAFTNENFEI
                     EKFTKENEGYKNEYNKWVEVWKKYLPFINFLGGMMTVVIILVGGILVINEKLSYGQLV
                     IYTGLAGTLSVPINMANWLSDQVQKFFTSGEKVIELMTVEAKITDTEDSIAFDKVNGQ
                     VDFNNVSFSYNGEKVLKDINMHIEPGQTVGIMGATGSGKSTLANLICRFYDCNEGEIL
                     VDGTDIKKIFKKNLRNNVAIAMQDTFLFSDTIEGNIAYGVPDANMESIEKAADAAGAH
                     EFIVKLSEGYDTIVGERGVGLSGGQRQRVSLARALLRNSPIMIFDDITSSVDVETEQK
                     IQETLKSVYSGKTTFIISHRISSVRNADIIFVLDNGRIAEQGTHEELLANKGYYYNVF
                     MIQSGKAKTLVEAGV"
     gene            23190..24029
                     /locus_tag="PK733_04365"
     CDS             23190..24029
                     /locus_tag="PK733_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09811.1"
                     /translation="MCTLGINVDTFNPRIFYIFKRKFGDQDATKYHCHDFISFIYVLS
                     GGCTYNLDGFLYQLKKGDLIICNPGVYHCRLMNTGQEVIEFHVGFSNISLADLQRNYL
                     IPENSNPVFKISRYEQDFLRCCSEIVAEQEKDEPGCEPLLKSLVMKLIVLFLRETSTH
                     RNSDKSAVFNFDSYDRLNIVNTIVSYINENYMNDISLDKISKNMYLSPVYISKIFKEE
                     IGESPINYLIKVRLAKALELLENGDLTVRAIAKSVGYDDAYHFSKLFKKYYGSPPSKF
                     RNK"
     gene            complement(24060..24980)
                     /locus_tag="PK733_04370"
     CDS             complement(24060..24980)
                     /locus_tag="PK733_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ09812.1"
                     /translation="MKLGFLTVCLGNMPLEEKVKWAAENGFKALEVACWPRENDRDYS
                     SSDIDVANLTEKEAERIKACFKEYGLTISSLAYYDNNLHNDLNKRAFINNHLKKCIDA
                     AVMLETSMVGTFVGRNIDKTIEDNFEEYERDFGKLVRYAEDRGIKIIIENCPMEGWQV
                     RGQPGTISFTPELWEEMFKRIPNSNFGLNFDPSHLLFQLINYYPLVEKFKDRIFHVHA
                     KDAEVFEDKLKWYGVFNRHLRHGGGNGYWRFRMPGLGQVEWSRFINELKKIGYDGVVS
                     IEHEDPLYEGSEEKVKEGLLLGRKHLEERM"
     gene            complement(25131..>26181)
                     /locus_tag="PK733_04375"
     CDS             complement(25131..>26181)
                     /locus_tag="PK733_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09813.1"
                     /translation="CGGIANGKHMPSLRKLSNVEMVAFCDIVKERAEEAAQKYGTKDA
                     SVYTDYKELLKDSSIDVVHVCTPNKSHGFITVDALEAGKHVMCEKPMAKTSEDAKKML
                     EAAKRTGKKLTIGYQNRQTAESQYLYDICRRGDLGEIYFAKAHAIRRRAVPTWGVFIN
                     EEEQGGGPLIDIGTHALDLTLWMMDNYKPKYAVGTTYHKLGSKKNAANAWGPWDPEKF
                     TVEDSAFGFIVMEDGATIILESSWALNILQVGEAKTTLCGTEGGADMWDGLRINGEEF
                     SRMYTKIPELGKGGVAFYDGKAESPSDREARLWIESIINDTEPVVKPEQALVVTQILE
                     AIYTSSKTGEPVYFK"
BASE COUNT         7500 a         5489 c         4072 g         9120 t
ORIGIN      
        1 ctaacagcag tcatctgttt gtcttttatt aatacagacg gtattttgtt ctcaatttta
       61 aatactaagt tagtgctacc ggtattttca ttgcttttgt cgtttttaaa atttactctt
      121 gtatttcctt ttaatgtata tgtgttttgg gcaagtatgc ttgcgacctt atattttaag
      181 ataaatattg ctttgccttt attataatct acagacttta cactgcccct tatgtcgctt
      241 cctagtgata ttgtccagtc ttttatatcc tcccttgtat accttgctgt tttcttgaca
      301 ggatccttat aatattcttc atccagcagt acacctgata cttccacttc cacctctatt
      361 tcgctgtcaa ggtattctag tgtatttccg gagctcccgg aaccaaaatc ttttattgca
      421 agtcggtttt caggtgttaa tatctttacg gaagttgcta catctgtcat aagcttgtcc
      481 acatggtcca gcttgtctat agtgaaagta cggtatagga gctggttgtt atatttgtac
      541 catgctctgg cttcacccgg gtagtttatg gtgggtgctg acataatatt tatatacctg
      601 taagcctccc tttcactatt gttctctgca ttctcaaagg taagggttat gccgtccttt
      661 tgaatagggc ggttaatcca ggccttggtt ttctggtctt cttgatttct cggccattct
      721 gctgcttcat tataagggct tattttttcc aaattatagg tatccttgct ccatggtcta
      781 tataaccata cattatcatt gatacttgaa ccagtattta catctattgt ccaccatgta
      841 ttattatatc tattacctgc tacatcaaag ccatgataac ggtactctcc ccttactcca
      901 tttttactgt aaaaacctac tccataaggg ctgtctgttt cagccttgaa atcatttgat
      961 ctggagttgg cagttgaata atcaccatat actacaatat ttttagcaaa ccacagcctg
     1021 tagttaaaag ggaacacact tccgttaact gttatggttg aaggtgttcc cggtggaaga
     1081 gtgctattta tgctctcaaa tgtgttttta ttgttttcat catagaatac agggaaatag
     1141 cctttttcat aatacatttc gctatcatct tctgctttta cagtcactaa tgatatatcg
     1201 ggtagagtaa acatagatat aagtattgat attattagta ctaagctaat ttttttagtt
     1261 ttcttcatta ttcttcacct acttttgata tagccattgt tttttatata ttttgctttc
     1321 tagtttcctt tatttttaat ccttatatac ataccaaaac catcatcgct attatgttta
     1381 gtaataatta ctattctatt gttaaggctg tattcttttt gatatggaat tccttgtctt
     1441 ttaattgcca gttcaaggat gtggtcaaaa tcttttattg ccctttctgc ttcatcttca
     1501 agaagatggt taagcaaatc aatgaataca tctctgtgaa gttctttggt agctttggca
     1561 tcaaatatgg taatatcata accatttaat tcccattcag ccattcttat actgaaacta
     1621 cagtctttaa atatactgct ttcttcatag gcttctttac ttttatagag cctagtacct
     1681 actacttcat caggagcata atatcccctt aaaggtaacc ctttactttt cttcgcacta
     1741 tcactcatta ctcttattac atctattact tcggttttgg aaggcgggta tatttcgtat
     1801 attttgttct cataatcatc atagaactta actatctcac tatccttcgg cttcattggc
     1861 tttctcatgc cttcatccag gtacctcatt acaatagcac ttccctcgcc tctagtaagc
     1921 gtctttttag gcagaaagct gccattgcct atgcctgcta taaaacccat tgaataggca
     1981 tacaacacat gctgcttata ttcatcacct atatcgtgat aatcgggtat tcttaatttt
     2041 gcataacttg tatggttggg tataggtgct tcctcaattt ttaacgctgt cttcaccatt
     2101 atcctggcag cttcctccct taaaatgggt cttctgtaat ctgtaaattc cttttcctca
     2161 ataatcttct cttccctggc cagctttata tatggttctg cccaatactc catcccttcc
     2221 tctaccttat gacccattgc acgcaaggtc atttttaaaa attcgtctac cttcaaagta
     2281 tcctcaggtc caaacctgct gcctccatat cctgctacta tttccagcaa ggcaagtttc
     2341 cctatatact ctttactcca gtgatccttt atatcattaa atctctttgc agcctcttca
     2401 atatcaggat tttctattgc agaaagcctt tctaaaactg tactaaaacc agacccggag
     2461 gttggcatct ctgccaggac tgtgatactc cctgacagaa aaataactga tgtcaataga
     2521 gctattgcca cagtagacgt gaaaagcatg aaagccattc ttaccattaa aaaccttcgc
     2581 atcatattca ttaaactcct catccaacta ttattgataa tatattacaa cagcacaaca
     2641 gcaacagatt agcatccctg cagaaccatt tttgcgttat atatacctta caacaaaaag
     2701 ataatccatg tctgcaaatg taattctatc cccatctttt atctcattct cagtattgca
     2761 gtcaattcta atatcgttaa tgaaggtacc attccttgag tttagatcct ttaaataata
     2821 cctgttttcc cgggtaatta tttgagcatg tactttacct atagtatcgt tttttattgt
     2881 atagtcaacc tggttttcca gtctgcctat gaggaaatca gccttattaa caggtacttc
     2941 ctcctgtaga tttcctttca tatttattat cataggcttc tgttcctttt tgcctgatag
     3001 cagtacagtc tttccaccat agtcaagagc agttttagct tcatggcaaa aatagctgtt
     3061 tgatttcatt ggttctttac ttattggctc cctggttatt tgtttctcag gctttccaaa
     3121 gccagaaaga ttcaaccctc tatatggctg ttcctccgaa ttaataccct gccgggcatt
     3181 agactgggca ttcatatcct gccaggcatt tgtatgctga gtattcatat cctcccggat
     3241 atttgtatgc tgagtattca tattgtcggc atttatgttc ttagagctta tattctgagt
     3301 gccagaggta aagcacatgc cttctggact gctttggggc ttgtctacgg tttttcccat
     3361 tccaaacagg tttttgaaaa gcaatgtgtc tatagcaata attattaaag caactgcagc
     3421 atatgtagtt ttaatattcc cgtttaaaga ttcgaaaaat ttcttactta ataaaataag
     3481 aattaggata ataagctgtg aaccaatagc catccatact ttaccggaaa tcttgctatg
     3541 attcaatatc agatttccca acaatcctcc ttgttcttca tcgtttgtat tatagctgca
     3601 ctgcttaccc ttgtcgtcta tttcagttcc cttccctctg tcacctttcc cgcaccttac
     3661 ctcatcccaa ctattcactt ttcctgtctt ttcctctaat gccgcttcag aatttgcact
     3721 ccctatgtct gatacaccat ccatacccat tgtatctggt gcacttttta tgctcatttg
     3781 atcttcggta tttgtatatt catcctgatt gccaccagta gataactgca ttaataacct
     3841 gttaaagccg ttaatattaa aggtctcacc tttcaagtag ttgatgattt tctgaataaa
     3901 attgtctccc gtattttcgt cttcaatata tgcagtatta acaattaaat taataacaaa
     3961 ctttcttaag atctggttaa cttcctcatc cattattgca ggtatataaa ccatatttat
     4021 ctcgaagttt gccggattta tgtatatata atcaaccctt aggatgtagc acttgttgta
     4081 taaaagataa tttacacccg aaagcattat acccgtaatc tggcttaaca ggtttacaaa
     4141 ctcctcttta tttatttttt ttcttgacaa gaactgagag agaggcattt gggaagtaat
     4201 attataatat aagcatgttt cattattttt acgtcttaag tccattggca gcaagccggg
     4261 tatccgattg ttaataatca tctccacttg ataattcaat agtttattca cttccggctt
     4321 tattaccagg tagctggaga agtcatctct ttcatatatt ggccttatcc cgtaattctg
     4381 gatgtccacc atcatcacct cactatacta tttccccgat tatttattac agcctgcctt
     4441 tttaattaat tgcgttacca tttcttatcc cgttgctttt aatatcatta ataatactgt
     4501 catcgccaaa aacactcttc atgactttaa ctgcaaactt tatgatggca tctctgaaaa
     4561 ttagagctat ccccaccaaa actgctatta ttacaactat ctctacagtt cctactccat
     4621 cttcatcctt cataaagcgt ttcatgagct cagtcatata tctgccccct ttgtatttaa
     4681 tattgttata atactttgct tcactgtatc atcccaaatt tttactgtaa gactcagatt
     4741 ctacctgtta attgcgttct ttcaattaca tggttacaat tactttcgta caatcacatt
     4801 ttaaaaatag ataaaaccgc aggagttgat actattagaa gaattgctac cagcataaac
     4861 atcataggga ttaccatttt tgttgaggcc tcctctccta gttttttagc agtatttttt
     4921 ctcatttccc agcattcgtt tgaaagtact ctgaaaattg aagcaagttc tgaatttccc
     4981 tttcggatat tctgtattac tatggataca aatcgtgcaa cttctaccgt tctgcatctt
     5041 ctggaaaaat cctcatatgc tttaatctcc gatttcccgg agtttatttc agcaattaca
     5101 atttccgctt ctctatataa gtccgactgc tttttattct ctctaattac tttctcccag
     5161 gccctggatg tagtaagccc ggcattgaca agaagtgtaa gcttatttag aaaatccggg
     5221 aaatcccact gaattgacag atgccttctt tttaatttac tatccagttc cctatcactt
     5281 aagattacta tcatagccaa aaaacatatg cttaatactg cataagtcat atcaatagct
     5341 gtaccgagtc ctatcagcag aataacaaat actcccaaag caaataaaac cattttgttg
     5401 gcccagtgta tctttaagta atgccacgag tttttcgatc catatatttc acttattttc
     5461 acaagcagcc ttctatcata gtttgatacg tatctgcagc ctatagcatc cataatatat
     5521 agacctattg ggagcagatt tttgagtggg aattgttttt tatccagcga ttctaccaga
     5581 ttgtcatact ttccttttga caaaatatat aaaactaaaa ttgctgttaa gtatgctagg
     5641 atagctaaac tcatcttttc cccctgtatt acacatctat atccattatt ttttttgata
     5701 taaagtatga tatagttatt aaaagtgccg caatactcat aacaatcctc cctattggaa
     5761 cggtgtaaac aggattaata taatctcccg ctgtagctga aagcaataca agcattatta
     5821 caggcataat attcagcact ttcctttcaa aattcttcct ggcaagcatt gtatcaatat
     5881 cctgcttaat ttcaattctg tcattgataa tagatgttgt cctttttata gcatcggcta
     5941 tatttcctcc tgttcttttg caaattctta atacttcagc aaaactttgg acatcctcga
     6001 ttcctgaacg ataggcaaaa tcatcaagag cagattcaat agtttcattc atttccagtt
     6061 tcctcactat gtagattgct tccataataa tataggtatc agggcttgga tacaggatgg
     6121 ccaggtcatc cacagcccca acaaatgcaa gttcaacaga ttttcctgct gacaaggacg
     6181 aatgaaggga ataaagcatg tctttgaatt gaaggtttag ttcatttctg cgttttttaa
     6241 ttactttttt attttcaatt gatggccagg caaaagctgc aagactcaat atcaaactaa
     6301 acaaatgact cttataaaat aaataaacca gaaagtatat tatgggcatg acttttagaa
     6361 tatgatagat tttttctttt aggggcataa tatatatgct ataatcgatt atattgttgt
     6421 cttccctgca cgagttttta ttattacatt tttccgcacc ttttttcatt tgtaaaaata
     6481 cccccagtat caataatgca ttcctgccgt atggaatttt gctgttttca ccattttgtt
     6541 ttctgttttt acaaggctgc ctttaacttt tccatcttca gtatctccct catcgctgaa
     6601 aacaaaaagc ggatttaata caatctcacc attcttaaaa ccggaaactt cagttatttc
     6661 aacagttttc ctggttttat cccgcattct tgccaaatgg acaattacat caattgcaga
     6721 cgcaatctgc tggcgtattg cagtaagtgg cagaggagca gcgctcagta ccatagtttc
     6781 aagtctgctc aacatatctt ttgaagaatt tccatgtcct gtagagagtg aaccttcatg
     6841 gcctgtattc atagcctgaa gcatatccag agcctcttct ccccggactt ctccaacaat
     6901 aatcctttct ggacgcatcc ttaaagaagt ccttataagt tctctaatac ctatctttcc
     6961 tctgccttcc atgtttccgt tccttgtttc caggcttatc aggttttgta tcccttttat
     7021 ttgaagctca gcagaatctt ctatagttat tattctctca tcacccgata taaagttaga
     7081 gagagcatta agaaatgtag ttttaccgga gcctgtccct ccacacacaa aaatgttata
     7141 tcttgccttt actagatttc ctaagaaatc tgccgcctct atggttatag aattccattt
     7201 aataagctgc tccattgtca gaggcttttg aggaaatttc cttatagtca taacaggtcc
     7261 attcactgct atcggtttca atactacatt aacccttgaa ccatccttga gacgggcatc
     7321 aacaatcggc gatgcttcat ttacaactct atttgctttt gaaactacag attgaataac
     7381 atcctccaat ttctcggtac tttcaaatct aacatctgac ttgtatattt tgccaccctt
     7441 ttctatgaaa atattaccag gaccatttac catgatctct gtaacggatt catcatcaat
     7501 aaagggctga atagctccca gccttctcat agagttaaaa acagtttcta ctatctcatg
     7561 cttttcatta atactgaaat agaattctcc agatatatca aatactgcat ttgttattag
     7621 ctctcttatt tcagaatctg aaatatcttt gccacaatca atgtttctgc ttataatact
     7681 gcttacagta tttcttatcc tacataatat atcactgtta ctatatatat caccgctact
     7741 atcaaacacc atcaaatcta cctattagct ttcttaattc cctattaaag ctgttaattt
     7801 catctgcctt ccattcctgc aattcaagtc tttcctcaaa tggcagtttt atatcaatat
     7861 ttttccccgg tgttgagagg agagagttta gtctattaat atcaaatgaa ctgccttctt
     7921 cccacttatt tattattata gtagctttat ctataaagct aatattcttt tttcggcaat
     7981 aaatctggag gctatttaca aagtgctcca tcttgcttaa tgcaaccata tcagtagttg
     8041 taaccagaac tatttcatca caatcctcca taacagctgt attcagctgg ttaaaagaac
     8101 tcgccaggtc tgcaataata atgtcatatt gggccattaa cttaatttga tctacaagat
     8161 atttaatatc acaggcagat atctcatcta attccatggc attatccgga ggtggaaaat
     8221 aatatatatc atattgcaag gccttgcatc gtgcagcttc aatcttgtga ttaatgtctg
     8281 tatccttctc ctttaatgaa aacagtattt ttgaaaaact gttttgagct tctttttcag
     8341 gtaggcttcc aggaaaacaa tttacagaac gtatggattc aaggtttaag taaaatactt
     8401 tcattccttc cctagcatac tgtatagata aattagctgc aattgtagtt tttcctgttc
     8461 ctcctgcagg cgaataaaca ctaaccatcc tggttttctt cacttctttt tccagcagca
     8521 tattgctgta tttttccttt tcatatatgt tagagatatc tttcaccagc ttttcaccgc
     8581 tgcagtattt gtaaatttcg tttttacatc ctgcaatttc cttgctaaaa tactcggaaa
     8641 ccgtcattct ttcagagatt cgtataatca caggcaactt gttatcctgt tccaggccat
     8701 ttagattatc gcagaattcc tcacccacaa gtactatatc aataatacct tcatcctccg
     8761 ccagaaaccg tttaaaatgg tccttatttg tgaaagaatt tacctgaaac ttatagggat
     8821 gtttttccat caagtaccct gtcaagcttt cgatatatgg ttcatcctta tcagcaagta
     8881 ttagtttcag tcttctcaca tattccaacc ccctcgccct gcagtatatt gtaagtgtta
     8941 cattatgtat tgttttctat attatatatt atattcctta atatgtcaac tataaatatt
     9001 agttaaatat ttaatgtttt ttaaaaaatc tctaaatctt ttattgggta ataatacaaa
     9061 gttttactat tttattgccc attgtattga cgatatatgc ttcaaatagt atattataat
     9121 gtatcagcat gaaatcaaaa ttagaagacg aaaaaggcaa cgattaataa ttcatttttt
     9181 gtgtatatcc gaatataatt attataagac acttaaatgt gcctgggtta atataaatga
     9241 ggagtgattt ggtgaaggtt cataatctac ttatagttac tattaacttt cttgaattgc
     9301 tgattatatt atatgaacgc aggaaaatac agtatagcac tttttatgaa tgctcaaagc
     9361 aaaaaattaa gtttttggaa gacaatatta ctgaaatagc ttctataaaa aaaagagaag
     9421 aagccaggct tattcttaat aaatgtaata aattcaatca aagttcagaa tcagatttcg
     9481 cattgtaata gtctacaatg agggcatcta attctttgct gattttaagc aggttaccag
     9541 agttgatttt ttcatcattt atcgaatccc ccagcttttt ttgaagcatc attatctctt
     9601 cttttaattg tgtaaccttt tccccatatt gcatttttaa aacctcactt aacgcttctc
     9661 atcctttgtg tgcatttccc ctataatatt agatggtttt ttattgttat ttctattttt
     9721 gcatctgtta ccctaatacc aaatattaca ccatttccca cggattttca acattatttt
     9781 gccatatatg ggcataattt ggcaacatac catgattgaa gcttccagcg tggaaattat
     9841 ttcccaactt caagcttgta ctataacttt tttccatcat ttcaaagtta actcaattag
     9901 ctttgcctgc attatgactg tcattttcac tgtatatata ttttatttta atactgcttt
     9961 gtcaataagt gacttatata tactatatat accctaatta atgtattcca aagcattttt
    10021 ttctataatt aagtcagaaa tattaagttg ctttaaaaag gattttacat tattctggta
    10081 atctcttatc tttaatggtt tcccatcttt tgcggaatat atctcaccac gttcatttat
    10141 ataaatataa ttttcagtta ttaccgtacc atttcttaga acaacatacc cgtttttagt
    10201 attgagaagg tctttgccca tagcataagg ggctttgaaa tccatcaaat tggcaatggt
    10261 aggcaaaata tcaatttgac ctccggtaac actgatagta tccccattta tttgttctgg
    10321 gtagtgtatt attaatggaa ccttttgcag ttttatccac tgtgtatcac tataatccat
    10381 tcctaaaaaa tccataagtt ctcctgaaag gtgtttaggg actcccgaat ggtctccata
    10441 aacaacaaga agtgaattat tgtaaaggcc cttttgtttg agttcttcta taaatttccc
    10501 cagacaactg tcggcataat ttatagcttt gatgtaattt cctatgaaag caccttctaa
    10561 ttcacctgct tcaaaattat aatcttcaaa gaacgtaaat gggtaatgac ttgaaagagt
    10621 tataaagaat gaatagaatg gcctggaagt atctatttta tctaaggatt gattgaaaaa
    10681 agatgcatcg cttaaggcct ccccatccca tccggcaaag tcatccatct cgtagtatga
    10741 cccgtctata aactctttaa accccaatgt cttgtacatt tccgtcctgt tccagaattt
    10801 ggcggtaaaa gcatgtaatg cataagtatt gtatccttta ttattcataa tttcaggtag
    10861 agaatgatat gtgttctcgg caaatcttac atacactgct ccttcttttg caggatacag
    10921 agaagtgttc agcaaaaatt ccgcatcaga agtattgcct cctgcaactt ggtagtagaa
    10981 gttgttaaaa taaaggcttt cattaattaa tttatttagg ttgggcgtta tttctacacc
    11041 atttactttc ctgttaatca taaactcctg aagggcttca acctgtatta ctattaggtt
    11101 cttatccttc gcaatacccc tgtattttct tccaggctcc tgcttgttgc caaaatattg
    11161 tttaatagaa taactctcat tttgtgttaa tcccttattt ttattaaaac tttctttagc
    11221 atataatttt aagccatcaa agtgcgaata aaggataccc attttttgag ttatataatt
    11281 gctgttataa ataagaatat ccatatcggt tttactatat atggatataa agcctgccac
    11341 acctagcgcc agtgataaaa cgccagctat tagcctattg gcagggttaa ctttctcaat
    11401 accctttttt ctgaatttta tcagccatat aagcaacatg ggtatatcca gtatatatat
    11461 tatatcttta actttaaata agctctctat actttgctga atagaactga agagcttaat
    11521 atcaatctgt aagataacag gaattgataa tattccatag taatagcgaa agaagtttgt
    11581 atcacctgta agtattgatg atattattaa attcagcaca aaaatagctg ttaacctctt
    11641 cctgttaaaa agcataacta ttaagccagt aataataatc agaatacaaa acgatgaaaa
    11701 aatcattgca agattatcat gtgagaaata aggctgcctg tttatttgag tagtaaattg
    11761 aaaatacaag cacttacaca ttatggagat aaagagatat atccagtaag aatactcatt
    11821 ttccaatacc ctggctacaa ttttattttc ttttatttta gtctttttat taataattgc
    11881 ttctgtcatt ttaagcttca ttcctcctgc atttagcaac atgtctatca aagtgaaaag
    11941 gttaaatttt tcacaaaagg ttaattatag aaaatagctg cttatgtcca taatagctgt
    12001 taataatcgc tatttattgt ttaattcaaa aattagtgtt atctttgccc aattatacgc
    12061 cgcaaaacca cctgcaaaaa ataataataa taatacattg cttataatta atgtaacatc
    12121 aataacaaaa tttcttaatt ttttcataaa ctattatacc ttattattcg gctaaagatt
    12181 gttacatttt agttaaaaca agtaaaattt tagtgaaaat attgaaagta agatgcctgc
    12241 acaaaagtgc aggcatcttt aagttagtta tttatatgca tcaggtattt attgggtaca
    12301 tttgatattt atatttatcc caaatattta cgtaaagtat tccttacagc attttctcct
    12361 gcaatcattt cagtgaaata ctgttctacc ttttctccaa gcccaacctc atataaatct
    12421 actccaaaca actcagtatt tggtaatatg ggttttaatt ttccagcagc actttcagga
    12481 tttccaagct taatatctgc aaaatatggt tttaatgttt ccagcatagg atccgagctt
    12541 agtgtcattt ctttgcctgc atcattaatc cccataaggt atctgcacca tccagccagt
    12601 accagcggta tatacttcaa acttgctgca tctagatccg ggctgttttt gtaagctgca
    12661 atagtctgcc caaatcttat ttttaacttt tgggaggtat ctgttgcaat tctttgagga
    12721 gtatcgggta tgtatgggtt gggaagtctt acctcgataa cttcttttgc aaatgcaaca
    12781 gggtcaataa cacctgggtt tataacaacc ggcattcctt cctctaaacc gatcttttca
    12841 accaattttc ttaaaagatc atcctgcatt tcctgggcaa tagttttata atccagtaag
    12901 cacccatata ttgcaagagc agtatgcaga ggatttaagc aggtacctac tttcatcttt
    12961 tcaaccctgt caactgtatt cctgtctgta aaaataactc cggctttttc cagaggggga
    13021 cggccattag ggaagctatc ctcaataaca agatattgag gtccttctgc attaataaaa
    13081 ggagcaataa aagtgttttt atttgttctg ataatatcca tcccgccaat tcctgcatta
    13141 tccaatacat tttttacttc ctctgcaggt ctcggagtta ttttatctat cattgaccaa
    13201 gggaaggaaa cttttttact gtcatttaag taatcaataa attccttttc tacaagtccc
    13261 ttttcagccc atacccttgc aatagtatta actgaagttg aaagcctttt tccgttttct
    13321 gcacagttat ccatacttac aaaagctata ggaagttggc cgtttttata tcttgtatat
    13381 gccagagagg caacttttga cataacatga ataggatttt caggaccgac ttccatatcc
    13441 ttcttaacat cagccagaaa ttctccggaa agctgattta tgttataacc tttctcagtt
    13501 attgtaaaac tggccatttg cagtgatggg ttggtaaata ctttttttaa tcttgcccaa
    13561 tcctctcccc ttgaagggtc tccaactaaa gactcggata tgcttgcaat tacttttttg
    13621 gaaaagcttc catccggata gagaaccaca aggattccca tgttgtcata tgggtcatat
    13681 attctgtcaa ttatttcaaa atcataggtc tctgcagcaa taataccttt ttcagccagg
    13741 cctgattcaa gcagttcctg ctgaagtgcg gcaataaacc ctctaaatat attccctgcc
    13801 ccaaagtgta cccatatagg atcctcggtt gttttagaaa tcattttatc cagatcaaat
    13861 tcaggaatat ttacactggt gttttcccat agttttttat ttttaatatt ttctttgttt
    13921 aaatgtacca tatttcagcc tcctaaataa tattacattg ttctgtatct tctcgggtag
    13981 tttcccttac attgttatag gttttcaagt tattcatagt ttttaggtta ttaggttatt
    14041 tatattatac acaatatttt atttatttta aaggctataa ggtaaaatat atcagctgca
    14101 tgctaattca tatattgctt gggtacatat ttctgcatga tgcatgagct catcaatgga
    14161 cacattttca tcaggctggt gcgcccctgt ccctgccccg ccaaaagcta caccgttatt
    14221 tttcaatgtg cgcgcataag ttcctcctcc catggaaagc agctttgcct cttctcctgt
    14281 tattttttca taagcagtaa ttagccttat tattagagga tgagtttccg ggacatataa
    14341 tggttctgaa tgattcaata cttttactac aataccttct ttttcagttt tttcttttat
    14401 agctgctaaa acttcttcca ttttgcatgt cacaggatac cgaatatcta taaatgcttt
    14461 cgtgcctgta tcatctttaa taatcttccc caggttcagg gttaattctc cggattcttc
    14521 atcttttagt gcaataccta aagaactccc cgttgtttct tctccaattt tttcatgcaa
    14581 aaatttaagg aatccatcca gatacctttc tcttcccgcc atattcagaa aacttagcat
    14641 ccgatagata gcattgatcc cgttttgagg gcttgctgca tgggccggtt tgccctttac
    14701 caatatttca ataccttttc cattaatatc attaatatgt gaaaccgaag tattgaactt
    14761 ttgtgcaata tccttcagta actctacctc tcccggcttt attttatctt ccgggataaa
    14821 agctctgcac tcagggggta ccatattaat aacttcacca ccccggagtg tataaatagg
    14881 gttgttatgt agactttcat tttcaccttt ttcaaaaacc aggtggagaa tccccttttc
    14941 cctgttaatt ataggataat ttgcatcagg tgaaaatccc atatgcggta caggttcttt
    15001 cgagaaataa tactccatat cactcatacc actctcttca ttagtcccca ggataatcct
    15061 tattcttctt ttggggacta ttcctgcatc tttaagtgct ttaaggctat atagggcaac
    15121 cacagcagga cctttatcat ccgatgtacc cctgccataa attttacctt catgaatttc
    15181 gcctccaaat ggtggaaagc tccaacctgt accttcagga actacatcaa ggtgaacaag
    15241 cactcccaca atctcatcac cttcaccata ttccacatgt cctgcatagc catcaacatt
    15301 tttaaccttc aaacccagac tctcacccag attcagcata tattcaagtg ccttgccaac
    15361 accctgtcca aagggcttcc cttcacatgc ttcatctttt acactcttta tttggacaag
    15421 ctcttgaagg ctttttacga tgtcatttct gtgattttgt atttcttttc caataatcat
    15481 gatataccct cctttttaag gaaatatttg ataaatgccc ccctgcaaat ctagtgttta
    15541 catcattatt atattatatt cctgttttcg acacttgaag taaaaaataa agagcgaaac
    15601 tggcttaaac agcggcttcg ctcctatttg ttggcatgcg aaaatgttgt atggaacacc
    15661 tcttataaaa ttttttttat ttttttattc agctcctttg gttgataata tttcttgtcc
    15721 aatcctaagc catacagacc gttcaatgcc gtacatacct gacttccttt gtatgctgct
    15781 gtatagtaca tgtcctgcgt gttcgataca ttcatatttt tcaatgtgtc cagaatgtta
    15841 tctgttgtga agtgcgtgcc atactggtcc aatttacatt ctaataatct gtagattaat
    15901 aatgcggtgt aacaaatcat aaagtgtgca atgatacggt tttccttact gtggtgtatg
    15961 ggccttgcgc agaagttggt tttaagaatc cggaaacaat cctcaatctt gtagcgctgg
    16021 gaattgatgt tcaagatgtc ctttgcgtca tcatacaagt tggtagccac cgcatagtat
    16081 ccgtcgtatt tttcttcttc gtcaataacg gcctggtcaa tctcatagtg gtcgacagcc
    16141 ttctcgccat ccatcgtcac cgacgtcctt ttaatgaatc ttgagacatc gtgcggtccc
    16201 ttttttactt cctccacatc acgatacttt agaatgttcc gggcgcgttc aatttgggcg
    16261 tttcttatcc gacgctgata ctccatcact tttctggaga atgtgattat gatgcgttgc
    16321 ttcaggattg cttttgactt tacttttttg gttttcccat tcttcaggac tatttcctcg
    16381 tacagcccca aatctacggc gttttcaaca tccaaaactt tataggcaaa atcgttgtac
    16441 aaggggagat tctctttgtc aaagcggtcg aaatccttca tatgcttaat tgtgacgggg
    16501 gtatcgtccg ataatagttt gtagtcatag tcattaaata ccgcttgttt cagcgttttg
    16561 gacagctttt tgatggattg agtaacaatg aaggctctgc cgcccatgga gttgtattgc
    16621 ctgatgctga aggagccaag cccagcgtcc gcgcagtaga taaaccgctc gccaccgaac
    16681 atctttgtga ttttcttctc caaaggtatg gcacattttt gttcgttgtc ggatccggaa
    16741 ttgatacaca tggatatagg gatgccattg ccatccatga acagccccat ttgggcaagg
    16801 gggaggggct ggtgttgctt ggaaggacca tacttgcgga atccttttag aatctcacct
    16861 gtgacctcat caatacagtc ggggtcctcc gcttcgatct caaaataata gtttgtacaa
    16921 tcaaaatagc agaccgaagt gtccctgggg acgatgtttc cgctgttttt gaaaaggtgt
    16981 tcaatatatt cgtcatagtt tttttccagt atgtccatga aacgcaatat atgctggtac
    17041 ccaaaagaag gctgttcgta gtaggtgtcc agcttgtcaa aggtgcctag tttcgattcg
    17101 gggtcgagga ttcttgcata ggtaaggaag cggttaatgt cattacagga gaaggtggct
    17161 ttatggctcg tctggacagc tttgaaaaaa tccgcaatcg caagatcgtg atagatcttc
    17221 tgcaaaatta tgtagccagt atttaaaagg ttcgacttgg atgctatgtc actggtggcg
    17281 gtcagtttct catcaaaatc aatctcaaaa ctcatgctga gtttattgtg tttttgagct
    17341 tcgttagctt ccatcacttt ccttttagcg taagcaagag ggtcgggatc gatggccagc
    17401 agttcggaat gcttgccgat cctcatcaca tttctggtcg atgtcttttt accgttacgg
    17461 aaccccacct gaatgaagta agtaggatcc ttggactttt tatcgtaatt aagcttcata
    17521 tgccacacat ccttctcttt tgtgtattat ttgattataa cataaaattt aacgaaatac
    17581 aaccccgtac aacaaattat ttttaaaatt tgacaacaaa aaaatcagga aaggctttat
    17641 tggtaagcgt ttcctgattt taatagggcc tattttttag tttgaagtgt cgaaaacccg
    17701 catatattca agtgccttgc caacaccctg tccaaagggc ttcccttcac atgcttcatc
    17761 ttttacactc tttatttgga caagctcttg aaggcttttt acgatgtcat ttctgtgatt
    17821 ttgtatttct tttccaataa tcatgatata ccctcctttt taaggaaata tttgataaat
    17881 gcccccctgc aaatctagtg tttacatcat tattatatta tattaatata gtgttataca
    17941 aatttttatt atatattgaa taaccatgcc aaattatgca aatatatggg ggaaattgag
    18001 ggagaaaaag ctatgaaaca cctttttata ataaatcctg ttgcaggtaa gggtaaggca
    18061 ttagagtata taccaaagat aaaggacttt ttttcgcgaa ggcaagatga tatgtatttc
    18121 atagaaacga ctgaaaagcc gggtcatgct gttgatcttg taaaacaata tgtaagcaaa
    18181 gatacataca gggtatattc cgtgggggga gacggaacct tgaacgaagt tctgaatggt
    18241 attgccggaa gtgacagttc tcttgcagtg attcctgcag gatcaggcaa tgattttgta
    18301 aaaagccttg tgaattattc tccgctatat ctttctcaaa aggatgaagc aaaaagtgtg
    18361 cttcttgaaa aactagcaca tgggcaggaa aaacttgcag acatcggcag aattaacgac
    18421 agatacttca ttaacatatc ctcaataggg tttgatgcgg aagtagttta taatacaaat
    18481 agaataaaaa agctcccctt ggtcacaggt atattcgcgt atatccttgg ggtattttcc
    18541 acacttataa gctatagtaa aaatatttta agaatatcta ttgataataa ttctatagaa
    18601 acagacactc ttctggttgc agtagccaat ggaaggtatt atggcggagg aatgcagcct
    18661 gcacctgctg ccagtatata tgacggtatt tttgacatat gccttatcaa aggtgtagga
    18721 cggctgagga ttctgaagtt tttccccaaa ttcatcaaag gccggcattc tgatgttaag
    18781 gaagtatctt ttcacaggag taagcaagta aaaatttaca gcaaccacaa agtagccctt
    18841 aatgtggatg gagaagtttc tatggtagat ggtgaaattg actttgaaat tatcccggga
    18901 ggaattaaag ttgtaatccc agggtaagaa taatcgttat catggttaca gtcgtgaagc
    18961 tggatgtact tccaacttcc aattaacaac ttacaactca gcctctcgcc tgtatactct
    19021 aacttcccac cttaccctgc ctcattgccc gcgagatgct tgtactgttc cgtatagagt
    19081 tcccaataag cacctctttt ggcaatcagt tcatcatgtg ttccctgctc aacaatttca
    19141 ccgttatcta tatacataat tcgtgatgca tttttaattg tagacagcct atgggcgata
    19201 ataaaggatg ttctcccctg taaaagcttc tcaagccctt gctgcagtgc gatttcggtc
    19261 ttggtatcta tgcttgatgt agcctcatcc agtattagta ttttaggatc agccaaaagc
    19321 gttctggcaa aagatattaa ctgccgctgc cctgcagata aagtagaacc cctctcactt
    19381 acttcagcat aatatcctcc attcatctca acaatgaaat catgagccat tacagccttt
    19441 gctgcttcaa taacctcttc atcatcagca tcaagctttc catattttat gttatcaatt
    19501 atattccccg agaaaataaa ggtatcctga agcattattc ccatttgttt tctcaaggac
    19561 ttcaaggtta catcctgtat atttatacca tctataagaa tttcaccaga atttatattg
    19621 taaaacctgc ttattaaatt tactacagta gttttaccgg cacccgtagg gcctactaaa
    19681 gctattgatt ccccggggtt ggcagtgaaa cttacattat taagtatacg ccttccacct
    19741 tcctcatagc tgaagtcaac attcttaaac tctatcttgc ctgttataga cggcatttca
    19801 taagctccgg gaaggtctgc aaccgtaggc ttttcgtcca ttgtttcaaa aacacgttcc
    19861 aaatatgcca ttacagttac aatctgatta tagaaattac tgaggttgtt aataggaccc
    19921 caaaatcttc ctgtatagct agtaaaagct ataagcacac ccagtgatac acctccggca
    19981 atccatgaca ctcctgccat atatattaaa gctgtcacaa tagtcgatat attatcaaca
    20041 acaggtccta aaataaaact gctgtttact gctttcatcc aggaatccct gtatttattt
    20101 cccaggtcct ctaatattcc taaattaaca ctttcacgta cataagcctg agtaactttt
    20161 attcctgaaa tactctcatg tatataagca ttcatgtttg actgctttgc acttacatcc
    20221 tgccacgctt ttctctgcat gttttttatc aggaatacag ttagcaaaag tacaggcaag
    20281 cctgtaaggc taaggagcat aagccttgca tttatggaga acatgataac tattataaac
    20341 accagactta ttaaatctat tattacctgg atgagcccgt tggaaagtaa gtttccaatt
    20401 gagttaacat aattgataac tcttacatat attttcccat ggggtctgct atcgtaatag
    20461 gaaaaaggca attcctgaag atgggaaaaa aggtcaagac gaatatctct cattatactt
    20521 tgtcccacat cattaataag ctctattctg aatcttgaac aaactgctat tattgcaagg
    20581 ttcagagtaa aaaaggcaga caatacaaca agacctgcta catccttctc aggtatttta
    20641 gtgtctaaag cttctttaat taaatatggt cccaaaagtg aggctgatgt agttataatc
    20701 atcaaaagga gagtaagagc catttttctc ctgtatggcc ttacgtatct ggaaattctt
    20761 tttaaatgat taaaattaaa ttcactttca agcctttcat ctatatcata cttatttcta
    20821 gccatattaa catctccatt ccgcagcggt gaaaatcata cacctgcttc aactaaagtt
    20881 ttagccttgc cgctttgaat cataaataca ttataataat accccttatt ggccagtagt
    20941 tcttcatgtg ttccctgttc tgctatacgt ccgttgtcaa gaacaaatat tatatccgca
    21001 ttacgcacag atgatattct gtgggaaatt ataaatgttg ttttcccact atatactgat
    21061 ttgagggttt cctgtatttt ttgttccgtt tcaacatcaa cactggaagt aatatcatca
    21121 aaaatcatta tgggagaatt tctaagcaga gctctggcta gagatactct ctggcgctgt
    21181 cctcctgaaa ggcctactcc acgctctccc actatagtat cgtatccttc tgaaagcttt
    21241 acaataaatt catgggctcc tgcagcatca gcagcttttt caatactttc catattggca
    21301 tcaggcacac cataggcaat attgccttca attgtatcgg aaaacagaaa tgtgtcctgc
    21361 attgctattg caacattatt ccgcaggttc ttcttaaata ttttcttaat atctgttcca
    21421 tctacaagta tttctccttc gttacaatca taaaacctgc aaataaggtt tgccagggta
    21481 gacttgcctg atcctgtagc tcccattatt cctacagttt gacctggttc tatatgcata
    21541 tttatgtctt taagcacttt ttctccatta taactaaagg atacattatt gaaatctacc
    21601 tgaccattca ctttatcaaa ggcaatactg tcttccgtat ctgtgatttt tgcttcaact
    21661 gtcataagct cgataacttt ttccccggat gtgaagaact tctgaacctg gtcactcaac
    21721 cagtttgcca tatttatagg tacactgaga gtacctgcaa gcccggtgta aataaccagc
    21781 tggccatagg acagtttttc atttattaca aggattcccc caactaaaat tataacaact
    21841 gtcatcatgc cccctagaaa gtttataaaa ggcagatact ttttccatac ttcaacccac
    21901 ttattatatt cattcttgta cccttcattt tcctttgtga atttttctat ttcgaaattt
    21961 tcattggtaa atgctttcac cactctgttt ccactgatat tttcctgcac aacggaattt
    22021 aaccgggtaa cctgctcatg ccattcagaa ttcaggggct ttgtcttttg ggtcatcttg
    22081 aaggtataaa atcctataaa aggtgttact gatagcaaaa gcagagtaaa cagaaaattc
    22141 attcttacca ttataatcat cgcaaaaaca aaactaaaga ggctttcaaa tactaccgtt
    22201 gcagatcctg ccacaaattg tctgataacc tgcaggtctc cgcttatcct tgtcataata
    22261 tttccggttt tagtcctgtc aaaataacta aagtccatat tttgtattct ggaatataaa
    22321 tcattcctga catcaaataa gaagttctgt gaagtttttt caaccagcat aagggaaaaa
    22381 taccgggcta cagattttaa tgcaaccccc aatattaata tccctaaaag ttttagaagc
    22441 aattcatttc tgccgccttt tattacatca tcaacgatta ttcctgaaat atatggtgca
    22501 accattccta acaatgcatt tgccatcatg atcaggaata ctgctgcaat tgaaaacctg
    22561 tatttcttta gatatcccca aaaaaagcta aaaactctca taatctgtca cctaaaaaaa
    22621 attaaagact attgtgaaat tatcggaacc catgtcctat aaacgtacac ttttctatta
    22681 agatacgttc tctctctttt tccaataaac caatagtctc tttatttaat tcctttttca
    22741 tattagtcaa aaaatttatg catctcatat agcctggata ttcaactcca cttctgcagg
    22801 attagcaaaa ccatcctcat cctgtataag tgctttttaa tattcggata aaaatcccgc
    22861 tgtatttcct aaaacattat gatgtatatt taaagtaaat taaatgtatt attttaatct
    22921 gagcatgtat attcttatac aaaaagaagt tattctcaca ctttaaccca aaggcgtgag
    22981 atgttatcaa aaaacagctg tatgtaaaaa ttgaaaatcg tgtgatataa tgttaaaatt
    23041 attttatatc atactgcagc taaactgtaa gcctttaatg cgtaaaaatc gctgcggtaa
    23101 attaaggtgc tatattttct caaaaaagat agttgtcata tattgttttt tgagaaaaat
    23161 agtatattat gcattggatg tgatagtttt tgtgcacctt gggaataaac gttgatacat
    23221 ttaacccacg aattttttat atattcaaac ggaaatttgg ggatcaggat gctaccaaat
    23281 accattgcca tgactttatt tcctttatat atgtactttc aggaggctgt acttataatc
    23341 tggatggctt cctttaccaa ctgaaaaaag gtgacctgat catttgtaat cctggtgttt
    23401 atcactgccg tcttatgaat acaggtcagg aagtaataga gtttcatgta ggattctcca
    23461 atatatctct tgcagacttg caaaggaatt atctaatacc tgaaaactcc aaccctgtct
    23521 ttaaaatttc aaggtatgag caggatttcc tgagatgttg cagtgagatt gttgcagagc
    23581 aggaaaaaga cgagcctggg tgcgaacctc ttcttaagtc cttagttatg aagcttattg
    23641 tactttttct gagagaaact tcaactcaca ggaattccga taaaagtgct gtattcaact
    23701 ttgattctta tgacagactt aatattgtta atactatagt atcatatatt aatgaaaact
    23761 atatgaatga catatctctt gataaaattt ccaaaaacat gtacctaagc cctgtatata
    23821 tttcaaaaat attcaaagag gaaattggag aatcgcccat aaactattta attaaggtac
    23881 ggctggcaaa agcattggag cttcttgaga atggagattt aaccgtaagg gccatagcaa
    23941 aaagtgtggg gtatgatgat gcctatcatt ttagtaaatt gtttaaaaaa tactatggca
    24001 gtcccccatc aaagttccgg aataaataag gggacggcgt gtccccttac cttattacct
    24061 tacatcctct cctccaagtg tttcctgccc aataacagac cttcctttac cttctcttca
    24121 ctgccttcat acaacggatc ttcatgctct atgcttacaa caccgtcata gccgattttc
    24181 ttaagttcgt taataaatct gctccactcc acctgtccca atccaggcat acggaacctc
    24241 caataaccat tcccgccacc atgcctcagg tgtctgttaa atacaccgta ccatttaagt
    24301 ttatcttcaa acacttctgc atccttggca tggacatgga atatcctatc cttgaacttt
    24361 tctaccagtg gataataatt aatcagttga aataaaagat gcgatggatc aaaattgagt
    24421 ccaaaattac tgtttggtat tcttttgaac atttcctccc acaactcagg tgtaaaggaa
    24481 attgtacccg gctgtccgcg aacctgccag ccttccatgg gacagttctc aataattatt
    24541 tttattcccc tgtcttccgc atagcgcact aatttaccaa agtctctctc atactcctca
    24601 aaattatcct caatagtctt atcaatattt cttcccacaa aagttcccac cattgatgtt
    24661 tcaagcatga cagctgcgtc aatgcacttc ttcagatgat tattaataaa agccctcttg
    24721 ttaagatcgt tgtgaagatt attgtcgtaa taggccaatg atgaaattgt aaggccatat
    24781 tccttaaagc aggctttaat tctttctgct tccttttctg taagatttgc aacatcaata
    24841 tcacttgaag aataatccct gtcattttcc cttggccaac atgctacttc cagtgcttta
    24901 aatccgtttt ccgccgccca tttaactttt tcttcaagtg gcatattgcc cagacataca
    24961 gttaaaaaac ccagtttcat ctatttctcc cccttttaaa ttattgattc tgctgcaata
    25021 cccgctttag agacatttcc cctcgcaaca tctttccgat gctatcgaaa ttttgctgga
    25081 acacgacttt ctgcagtgga atcaattatt atatctgtta ccagaaccta ctatttgaaa
    25141 tataccggtt ctcctgtttt cgatgatgta taaatagcct caagtatctg agtgactacc
    25201 agagcctgct ccggttttac aacaggttcc gtatcgttga ttatggattc tatccacaat
    25261 cttgcttccc tgtctgaagg gctttcagcc tttccatcat agaaggcaac ccctcccttt
    25321 cccagttcgg gtatctttgt atacattctg ctgaattctt cgccattaat tctcaggcca
    25381 tcccacatat cagccccacc ttcagtacca cataatgttg tcttggcctc gccaacctgc
    25441 agaatattta atgcccagct ggactccaat ataatcgtag cgccgtcttc cataacgata
    25501 aatccgaaag ctgaatcttc aacagtaaat ttctcaggat cccatggacc ccaggcattg
    25561 gctgcattct ttttgctgcc cagtttatga tatgtagtcc ccactgcata ctttggctta
    25621 taattatcca tcatccagag agttaagtct aaagcatgag tcccaatatc aattaaaggt
    25681 cccccgccct gttcttcttc attaatgaac acaccccatg tgggaactgc ccgcctgcgg
    25741 atagcatggg ccttggcaaa ataaatctct cccagatccc ctcttctgca tatatcatat
    25801 aagtattggg actcggctgt ctgcctgttc tggtagccga tagtgagttt ctttcctgta
    25861 cgcttggcag cttccagcat tttttttgca tcctctgaag ttttcgccat tggcttctcg
    25921 cacattacat gctttcctgc ttccagcgca tcaactgtga taaacccatg tgatttgttg
    25981 ggagtacata catggactac atcaatagag ctatccttta acaactcttt gtagtctgtg
    26041 taaacagaag catctttagt tccgtatttt tgggctgcct cttcagctct ttccttcaca
    26101 atatcacaaa aagctaccat ctctacatta cttaatttgc gaagactggg catgtgtttg
    26161 ccatttgcta taccaccaca g
//
LOCUS       DAOSDD010000029        25551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_875_length_25551_cov_5.295458, whole genome shotgun sequence.
ACCESSION   DAOSDD010000029 DAOSDD010000000
VERSION     DAOSDD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 25551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25551
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_875_length_25551_cov_5.295458"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..954)
                     /locus_tag="PK733_04380"
     CDS             complement(34..954)
                     /locus_tag="PK733_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09814.1"
                     /translation="MNYYERIKKSIDYIESNLENKIDINFVAQTAYMSLSNFYRMFFT
                     LTGYSVKEYIRLRRISLASIELQNSDASIINIAVKFDFESGDSFSRAFRRVTGYLPSE
                     YRKHNRTFSFERIDIMDKYFDIQDNELLEKYPDIKVLKELAPMRVAYYCYYGKHPENH
                     AFAVIADWLNRNSLNANEQNLRIYGFNNPNPSSPDQDEYGYEVCVTIADNVIVNDDKV
                     KEKVMSGGLYAVTNVKRGENDNIGNEIGKAWNRFKNWLSDSKYVYGGHQWLEEHLGFD
                     DNANHIGGIDLYMPIALKSKEYDASELHRY"
     gene            1260..1814
                     /gene="spoVT"
                     /locus_tag="PK733_04385"
     CDS             1260..1814
                     /gene="spoVT"
                     /locus_tag="PK733_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOJ09815.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTMRIREGDPLEIFTDREGEI
                     ILKKYSPIGELGDFATKYAESLYKTSGHITCITDKDSVVAISGAPRKEFTDKTLSPGL
                     EKLISDKATVIVKSPEEGTIPITKDENGEKRYSSQLVTPIISEGDSIGAVIFLSTEPG
                     IRMGEVEEKLAKSAAGFLGKQMEQ"
     gene            2288..3082
                     /gene="mazG"
                     /locus_tag="PK733_04390"
     CDS             2288..3082
                     /gene="mazG"
                     /locus_tag="PK733_04390"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ09816.1"
                     /translation="MVKERYSLQDLTDIMTLLRSENGCPWDREQTHDSLKRYLIEETY
                     EVLEAIDLNDKDKLCEELGDLLLQIVFHGQIANEQGSFNMDDIINGICRKIIQRHTHV
                     FGKDKADTPEQVVNNWEIIKKKEKGMNTQTEVLKAVPKNLPALMRSYKVQQKAAQSGF
                     DWDDIEDVFIKVYEEIQELKDVYKSKNVARINDEMGDIFFATVNLARFLHVQPELALT
                     HTTNKFIKRFEYIEEESIKAGKKLEEMTLEEMDKLWNEAKKNLRED"
     gene            3166..3447
                     /locus_tag="PK733_04395"
                     /pseudo
     CDS             3166..3447
                     /locus_tag="PK733_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598975.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            3611..3853
                     /locus_tag="PK733_04400"
     CDS             3611..3853
                     /locus_tag="PK733_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOJ09817.1"
                     /translation="MRIDKFLKLSRVIKRRTLANEACESGKVKINGRVVKPSAEVKPG
                     DIIEITFGSKTSKMEVVTIAEHVPKSGAAELIRIIE"
     gene            4163..4456
                     /gene="yabP"
                     /locus_tag="PK733_04405"
     CDS             4163..4456
                     /gene="yabP"
                     /locus_tag="PK733_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOJ09818.1"
                     /translation="MAEDKKIIKPKIQNLILENRQKLSVSGVIDVESFNEECVVVDTE
                     MGILIVHGEDLRINKLNLDNSELNIEGDIISCEYSDKEGPRSKGIGLFSRMFK"
     gene            4472..4981
                     /locus_tag="PK733_04410"
     CDS             4472..4981
                     /locus_tag="PK733_04410"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HOJ09819.1"
                     /translation="MPISTSIQAHIFLYSILCGMVIGFVYDVFRIKRKTIKTTVLFIY
                     IEDILYWIIVMAIMFALIFYSNDGEIRGFIFIGALIGVILYMLLLSKIIVKFSLAILN
                     ILGKIIKVILFIVAYPFRLIYRVLSIPVKLLMKPAKKFTRSIKAYSRIKMVKISMWKR
                     SLRNRIKKI"
     gene            5179..5457
                     /locus_tag="PK733_04415"
     CDS             5179..5457
                     /locus_tag="PK733_04415"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOJ09820.1"
                     /translation="MNKRKGSIFGILVLIALIIYFSFIIIDQQKMLKIKETALMEIEN
                     KIKKEELVRNDLEKQKDMLNSREYIEKVAREKLGMVKPGEKIFIDINK"
     gene            5692..6117
                     /locus_tag="PK733_04420"
     CDS             5692..6117
                     /locus_tag="PK733_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ09821.1"
                     /translation="MLVEVGKVIDGKVTGLTNFGAFVRLSDGKVGLVHISEIADEYVK
                     DVNSYLKENQIVRVKIISVDEKGKISLSIKKANDGTKKVKSSRPEEIDWSKSDGKEKM
                     SFEDQLAKFMKDSDEKLQDLKKNFESKRGGGGYKKYANY"
     gene            6270..6358
                     /locus_tag="PK733_04425"
     tRNA            6270..6358
                     /locus_tag="PK733_04425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6304..6306,aa:Leu,seq:taa)
     gene            6371..6447
                     /locus_tag="PK733_04430"
     tRNA            6371..6447
                     /locus_tag="PK733_04430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6405..6407,aa:Met,seq:cat)
     gene            6633..7895
                     /locus_tag="PK733_04435"
     CDS             6633..7895
                     /locus_tag="PK733_04435"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOJ09822.1"
                     /translation="MKIKVIGSTKSGYCMPVEDALGFGGKSAGICYMPDSFESILGEN
                     IEKTEKRIQRTLKSGHHSVYDHPTYNLLLEDIPKILAMVLNNEGFYTTSEKSARYTKM
                     NPGKREKDLYEKWADILKEKIHEIYPQISNSHTEKLARENSRYMISVFTPTTLEYTVS
                     LRQMSYILGMMKKFVEKAEDTSFNTMLKESIGEFIESSKEFDIPYLNADIKGRELSLF
                     DARKNRRDEFGENYSVNYLGSFAQLGQAQRHRTLDYKMKVIEENPEFYVPPIIRAENN
                     NGDTALENTWLQDIKSVADMFPQGMLIKINERGTYENFILKAKERLCGSAQLEIAEQT
                     KDILLAYILNTRGTNDEVREYLAQYVIKKYAANAGNVDGMECDGNTFAADIAENTHGY
                     IAIPRCRFHDWKCTSPCIWGAKGLERLI"
     gene            complement(7938..10277)
                     /locus_tag="PK733_04440"
     CDS             complement(7938..10277)
                     /locus_tag="PK733_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09823.1"
                     /translation="MSITKFFEKYTKSVSKSLFIKFFLACTLISLFLVIIIGSYTRFY
                     ILKNLDSEIEKFERKKINETINTMNVLFGEMQKLSLNHAMNSKFLQFAYLPRDVIAEK
                     QLEVKSIQELLSNSINSSNYIRNIAIYYEKNDYVLDYSGPMGIESYYDKGWYDDYCKM
                     MASSLIMNTRKVNDRNRSSDYSHNNVITFITRIPYDNESKEGAIILSVDEKIISDLLK
                     NITYGDDHTLAFLVNKDGMVISSNKDNYIYENISNIINIPSGYMDLNNGSFDFKMQET
                     KMISYFETSRINGWKLFYIVSENKIYERSAYIKYMTIIILAILLLLMTAVSLAFSFRL
                     YNPIKKIILNIKKMMTVNDDNLSDVLMIQDSIKMLLENNQTLEKQLDDNKVLVREIFL
                     SQLISGKLFNRNEIKNRAEYFMLNLDFDFFKVAVIKIGCVSSNYISIDDLEFKKISLI
                     NIVDKVFNDLDIEVVCSQDSDDNVLILLKLYSVEKINEVEAIIEQALEDIKDSTEKFL
                     SIPVSIGIGRMYNDISNIGVSYKEAIEALNHKFLKGDESVISYTDIMGSKKEKLYYPI
                     EMEQKIISLIKLCDYDKTILILNDMINDIMDHNKSFQHIEICLSNISGIIERCIFDLN
                     LNIHEVFGKDDIPAFSIDKFKSIQQFMEWISSKFRTIIEYQIDQQKDNTKSFVSEIKE
                     FIEENYVEEISLVTAAAHFNYNSSYFCKIFKEKTGVSFWEYVSKVRIEKSKSLLIETD
                     NSIEQIAEMVGYNNRFSYIRSFKKYTSITPGEYRMKYQI"
     gene            10627..12189
                     /locus_tag="PK733_04445"
     CDS             10627..12189
                     /locus_tag="PK733_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09824.1"
                     /translation="MTGNPKKVVALVLSLCIILSLGFTGCVKKTVSEGSKEPSAQTTA
                     NNANTQAKPEEPYEFSIFRSTWTELNEDTDLVIQELNKKFNIKIKILTCPYEAWVEKY
                     NIYVTSGDVPDLSVTTGPGTMNFNAWAKQGIYLELTDLYSKHCPNIQKHISNDIIEAH
                     RINGGLYGIPKPSLSDTTAAIRADWLEKLNLSMPKTLDDLYSVLKAFMENDPDGNNKN
                     DTYGLCSEDTLNTIDFIFGAFGCHCPPNIATNWVPDGNGKLTSNLLEPGMKDAIKYVT
                     KLYRDKILDQEWMLTKSQAYVDKIHTGKVGVTKVAFQTMIAETETKIKANDPACKLEI
                     LEGVTGPEGKYARPMQKGFYMVSSISNKAKNPVKILQFLDYLMSTEGDKLIRYGVEGI
                     TYTEKDGKIVRNEEAVKKYGMESGHKFRQILQSIDIVIPGEDPRIPQLKEMAKVLYEG
                     PFYPAPTLQPASLKEVTTKQGPDFVKNSVAAIVTGNGDVDKEWDEFIKKWRESGGDQL
                     IKEINELYEANK"
     gene            12452..13474
                     /locus_tag="PK733_04450"
     CDS             12452..13474
                     /locus_tag="PK733_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09825.1"
                     /translation="MTQTTKNGNQARNQVWNKIPNKAQKHTLKQNWNRSGMDSGKSLL
                     KYIWRKKFMYSMLLLPLLYYIIFEYIPIYGIMIAFQDYAPGKPFIGSEWIGLKNFRLV
                     FSSQPFMESLRNTILISFYKIIFGFPFPIIIAFLLNEVRSVPFKRTIQTAIYFPHFVS
                     WVIVGAIIVSIFSINDGLLNQIFRHMGIAPVNIMSNPKYFRTILVVSSMWKEAGMQSI
                     IYLAAIAGVDVQVYEAAIIDGANKWQQTRYVTLPGIASTIAILFVLRLGSILSAGMEQ
                     TMVLLNPMVASVGENIDTYVYKVGLGQGDYSFSTAVGLFKSLVGYVLIMGSNWLSKKM
                     SGESIF"
     gene            13509..14378
                     /locus_tag="PK733_04455"
     CDS             13509..14378
                     /locus_tag="PK733_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09826.1"
                     /translation="MSKKINLFNVINYTLLFLFALSAIYPFIYILTISLLPVDEYLKG
                     GPFLFPRNISFEAYMHIFKNGNIFLSFKSSVIITLLGTFTNLFLTILCAYVMSKTKLK
                     GYKLFINIIIFTMYFKGGLIPSYMVMRSLGLVDSYWAVVLTGGISTYNMLVLRSFFIN
                     IPESLEEAAIIDGASEPYVLLRIILPISLPAIATIGLFYAVGHWNSYFTPLIYLNSRK
                     KWPLQVILREILFESTESMRTAGESTELQKSAIGPQLKMATVIVTIIPITLIYPFLQK
                     HFVKGVLIGAVKA"
     gene            14531..15742
                     /locus_tag="PK733_04460"
     CDS             14531..15742
                     /locus_tag="PK733_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09827.1"
                     /translation="MPYLYEDTLRKKAEEYILVLKRAGIDSEVLMLRDYMAKLSIKRE
                     EKNYGNLILDYKAKKDTFSTRTQELQDKSIEDELLILWDRCKLTAEPIKTAQSEEDRE
                     NRLDREDREDIKDGEDREGREDREDREDREDGEDYYEIYVDGSYINNCVGYGVVILKN
                     GQLLKELSGLVIDSVFTGSRQVGGEITAVMKALSWCKNNKIDKVSVFYDLENLKKWVT
                     GEFSTNIPMSKAYKDFVQNSGICIHWNKVPAHTGVKWNEWADELAKKGTKECTAEGIA
                     EFKMKFKTECETECETECTPERTIEHITECATTNPDSCGDLIEELEKTAEAFTLYLED
                     NNYIAEFLRIYNNMAAKICVEKDGETLGYIDIYNTRKLHLQPKYHEIRQPEIREEMEK
                     RWKAFSQNKQI"
     gene            complement(15758..18130)
                     /locus_tag="PK733_04465"
     CDS             complement(15758..18130)
                     /locus_tag="PK733_04465"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOJ09828.1"
                     /translation="MGMYIYQSKNNTMKNILLQSILVITITVVFALFLYNSFIKYLNI
                     YHTILELARVFIALSSFFSLWFTCSNTAFYNRILGFGYLRVAIFDIFHVFYFLKLNLC
                     SSTYFDLSTKYWLLGRITEAIVLLLYVKLYTSEFKKTSNHLKYINILTFFLNLNKWII
                     LFVDLFFTLCILYFTNSYTDKSIILLTERGVTPTKTIFECIIVAIYLLALYNSKHLVG
                     NHVSDEDHNQQHTLSNKYILISLILSISAELFFIFYISITSVTCTIGHILKIVSCFYL
                     FKSIFASTVFYPYTKLENAHKKLEETLRKSTSLTESLNDILDALPEAIIRYDQNGILK
                     HANRKFEELLGYNRNDLNGHTEKQLLEKFAVSSAYENSPGVIILQNEDSNGNTISNVI
                     RAYRTSAGDNIKLNVSLHKITDGFLKIFHDAKKEQELKNIHLQTQTILDAVTNCILMV
                     DKNKKFVLCNKALEELYGLDSKNIIGMDIDNFDKLMTSDSKELLNLVLQGHTTNQYHE
                     TSFTSSNGSIRNFLLYLAPIFNIDEEIIGGISVGTDITAMKEQQQILLQQEKLALLGQ
                     MGASIVHESRNFLSTIKGRCQIIENLVENEYIKNHISKISSNVDELNYIMSEFLYLSK
                     PREPEFAEVSMYDIIHSLKSMIETSSLIKGVNIEFDLSDEERYLMCDEAQIKQVILNI
                     CKNAVDAMDSNSNNLAILKVGTGYDKQANKMYIKISDNGKGISEEELQKIGTPFFTTK
                     RNGTGLGLSTCYNIIKDHNGRIQVESTLGKGTTFTIILPCIPDEYYEDRM"
     gene            18418..19371
                     /locus_tag="PK733_04470"
     CDS             18418..19371
                     /locus_tag="PK733_04470"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOJ09829.1"
                     /translation="MAINKSKVAIIGAGFVGASTAFEMALNQNVNELVLIDINKEKAL
                     GEAMDINHGLSFIGQISVTAGDYSDCANCDVIIITAGLNRKPGESRLDLAKKNLAVAK
                     DITDNIMKYYNHGVILVVSNPVDILTYMVQKWSGLPNGRVMGTGTVLDSSRFRYLLSK
                     RFNVDVRNVHGYIIGEHGDSQLPAWSATHIAGRNIDTYSAIPENGFTEADKLAIAEEV
                     KKAGIEIIKNKGATYYAIAITVNTIVETILKNQNTIRTVSSIINGYYGINNVAISLPS
                     IVNSNGIKEIFEMSLTKQEEADLMNSVKHVKSVLDKVKNIY"
     gene            complement(19649..20890)
                     /locus_tag="PK733_04475"
     CDS             complement(19649..20890)
                     /locus_tag="PK733_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09830.1"
                     /translation="MNESSQNKVRLGFIGLSGRGKSLLGTVLAMEDVEIPAVCEVYDD
                     RLQTGIEQVEAAGRKRPEGYKDYRKLLERDDIEGVVIATSWTTHLEIALAAMEAGKYV
                     GTEVGGASSLEECWELVRTSEKTGMPCMMLENCCYGRNEMTVLNMVKKNLLGELIHCQ
                     CGYEHDLRQEVACGIENRHYRIHNYLNRNGDVYPTHGLGPVAKCLNINRGNRFINLTS
                     MSSKSRGINEWVKANKGEDHPFAKTNFALGDVVTTMIKCAHGETILIIHDTSLPRPYS
                     RAGRVQGTKGIWMEDNNSIYIEGRSPEHTWESFEDYRNEYEHPLWKEYLDLGVRGGHG
                     GMDYLVLRAFVESVKNKVPTPIDVYDTAAWMAVTALSEQSITMGSSPVGFPDFTNGKW
                     IGRKSGPESKYSLENIYEDLF"
     gene            21108..21749
                     /locus_tag="PK733_04480"
     CDS             21108..21749
                     /locus_tag="PK733_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ09831.1"
                     /translation="MRVASIYTLLDLFKTNPNWNEGILVKNRDGSLRTNWYVASADAW
                     IHTFCNLLELDTIFDIQTWITNILIGVAAYNCQKNNSRIQRIVEQIKDVVNKRFAEDL
                     SVSSVVSEVFLLAGYATSLFKKETGISIMNFIIKVRIDAAKKMLQDPSSRVSEVSYRV
                     GYENIAYFSTLFKNIVGKTPKEYRDSYPDLSLENIEVIMKIMYHNNKYKNANR"
     gene            21818..22543
                     /locus_tag="PK733_04485"
     CDS             21818..22543
                     /locus_tag="PK733_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09832.1"
                     /translation="MIELSNLSKTFNGTIKAVDNVSMVIKDGEIFGFLGPNGAGKTTT
                     IKIMTGILNADSGSVKINGIDLKDDPLGAKAQFGFVPDDPNMFLRLKGLEYLNFMADI
                     YDVPSEVRKERIENLGERFEMTKALGDQIQSYSHGMKQKIVIMGVLVHNPSIWILDEP
                     MTGLDPRSSYILKEMMREHADNGKSVFFSTHILEVAEKICDRVAIINKGKILFCGNLE
                     EMREHFKSNESLEKMFLELTENE"
     gene            22612..24327
                     /locus_tag="PK733_04490"
     CDS             22612..24327
                     /locus_tag="PK733_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09833.1"
                     /translation="MSRFMINKRLFILTRVLLKNGGGVQEKKNKNKKRWKINKGLLMY
                     ILVGLAFLPLAVNIIFMAEAIYDSLAQIQQQGIVLSLGIGIVSFIMFFFGIFYVMNVF
                     YFANDIERLLPLPLKPSEIMAAKFFTVVIYEYLTEAVVMLPVLGVYGVKSGAGMPYYL
                     LGFLIFILIPVIPLSFASIIIMLIMRFTNIGRNKDRFKLIGGIAAVFISIGINAAIQR
                     ATMQSVDPKALQNMLIQGGNSLVGLAGRMFPGTSFAAVGLVNSSNIQGLSNVLVFVAI
                     TVIVFFLFMYLGELLYFKGVIGLSEAGGSRKIGIEELKRKSVRTSKLNSYIAKELRIL
                     FRTPIYFINCVLMNFLWPVFFIIMPLSQSGQSGGPEDMNMEMLKSLVQNDEYAGIILA
                     ISFAVILFIAGSNGVTSTTISREGQQLYISKYLPLSYVTQIKAKVLSGVAISAIGMLV
                     MILIMGAVLGLPLLIIVLSLLSGFAAIFLVSFTGIIIDLINPKLNWDNEQKAVKQNLN
                     VLFNMLISIGLAVAIVFVSIKFKQYMDVYATFAFLVLIFGLIDVVLYKFLNTKGVKLF
                     ARIEG"
     gene            24488..>25551
                     /locus_tag="PK733_04495"
     CDS             24488..>25551
                     /locus_tag="PK733_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ09834.1"
                     /translation="MFIENDIKDSVDNKVNTANNEICDKTNKTNTLNRESNLIINHSN
                     KLRYYLKKYIFSDRPFYEKLWMLALPIIIQNFMSSFLNIIDTIMIGQIGEIQIAAVGV
                     ANQYFLLCLLVIIGIHSGCSILMSQYWGKGDKKNIMKVQGVGLIVSFFAATVFATLAF
                     VFSKEIISIFNKEELVIAEGAKYLKIVCFSYIFTAISLGYAFASRSIGDALMPMIVTG
                     IAIICNTTFNYVLIFGKLGAPALGVEGAAIATNIARIVESIIIVSYIYGKNSILAVKF
                     IDLHGIQREYLIKMFKTVLPVVLNEGFWALGSVVRSIAYGRIGSHAMAAVQICNTVQN
                     LFNVAVFGMAAASAVMIGHK"
BASE COUNT         8777 a         3873 c         4829 g         8072 t
ORIGIN      
        1 cataatttca tagggaaccc gaagcccgct atttcaatag cggtgtagtt cacttgcatc
       61 atactcttta gattttaagg ctataggcat atataaatct attccgccga tgtgattggc
      121 attatcgtca aaacccaagt gctcttccag ccattggtgg cctccgtaaa catacttact
      181 gtctgaaagc cagtttttaa atctgttcca cgctttacca atttcatttc caatattatc
      241 gttttctcca cgcttaacgt ttgtaacagc gtaaagccca ccactcataa ctttttcctt
      301 cactttatca tcatttacaa ttacattatc ggcaatagtg acacaaactt cataaccgta
      361 ctcgtcttgg tcgggagatg atgggtttgg attattaaaa ccataaatgc gtaagttttg
      421 ctcatttgcg ttcaaactgt tcctgttgag ccagtctgct attacagcaa aagcatggtt
      481 ttcgggatgt ttcccgtaat agcaataata agctactcgc atgggtgcaa gttcttttaa
      541 aacttttata tcaggatact tttcaagcaa ttcattatcc tgaatatcaa aatatttatc
      601 cataatatca atcctttcaa aagaaaatgt tctgttatgt ttcctatact cactaggtaa
      661 atatcctgtt acacgtctga aagcccttga aaagctatct ccactttcaa aatcaaactt
      721 taccgcaata ttaattatag aagcatcact attctgcaac tcaatacttg caaggcttat
      781 cctgcgcagt cttatatatt cttttacgga atagcctgtt agtgtaaaaa acatcctata
      841 aaagtttgat aaagacatgt atgctgtttg cgcaacaaaa tttatatcaa ttttgttttc
      901 aaggttactt tctatgtaat caatggattt ttttattcgt tcatagtaat tcattgcgga
      961 gaactccttc attatcttaa gataagaata ttatagcatg tgtaaattac tattatccga
     1021 ctttttttcc aaaagtcttt ttattatttt tatagagaat acatgacata tggtaaattg
     1081 gcttgtttat cagtaaaact ttagatttat tcaccacttt ataaacataa ctgattttat
     1141 ctgttgtgtt tataaagtga gaaattcagc tagttttacc taaaatgtat aaaaaatcat
     1201 taaagaaata atactaaaaa aggaatctaa aatttgctat tgaaaggagg tagtttacat
     1261 tgaaagctac aggcattgta agaaggatag acgatctagg gagagtagta atacccaagg
     1321 aaattagaaa aacaatgaga atacgggaag gagacccctt agagatattt actgatagag
     1381 aaggagagat tattcttaaa aaatattctc caataggaga attgggcgac tttgcaacca
     1441 agtatgcgga atccctttac aaaacaagcg ggcatattac atgtattacg gataaggatt
     1501 ctgtagttgc aatatcagga gctccaagga aagagtttac tgataaaaca ttaagccccg
     1561 gactagaaaa gctaatatct gataaagcta ctgttattgt taaatctcct gaagaaggaa
     1621 ctataccaat tactaaagat gaaaatggag aaaaacgtta ctcatcacag ctagttacgc
     1681 ccatcatatc tgaaggtgac tcaatcgggg cagtaatatt tctctcaacg gaacccggta
     1741 ttaggatggg tgaggtagaa gagaagctgg caaagtcagc agcaggattt ttaggtaaac
     1801 agatggaaca gtaatataca atttatgtaa aacaagttat ataaaaaggc ttcctaggct
     1861 tctttagtaa ttacattgct ataatggaag ccttttttaa atgcaccctc gtatatacgc
     1921 atacttattt aattgcagct aatagatgca atatgatata atatcctcaa aaatgaggat
     1981 attatatgcc tccggcggaa tttatgtgcg tgcgaatatt tttgcttcgc aaaaaacgca
     2041 tatgcacaat tatatcatga atttaaaaaa attcatgata taatatcctc aaaaatgagg
     2101 atattatatg cctccggtgg aatttatgtg cttgtgaata tttttgcctc acaaaaaacg
     2161 cacatgcacg attatagcat gaaatttaga taattcatgc tataatagcc gcaatgtatt
     2221 tatgtttgct caacatgtta tctatagctt tagacaaagc aaattcgctg gaagggaaga
     2281 tggggttttg gtgaaagaaa gatatagttt acaggatcta actgatatta tgactctttt
     2341 gagaagtgaa aatgggtgtc catgggatag ggaacagact catgacagct tgaaaagata
     2401 tttgatagaa gaaacatatg aggtattgga agctatcgat ttaaatgata aggacaagct
     2461 gtgcgaggaa ctgggagatc tgttgctgca gattgttttt catgggcaga tagccaatga
     2521 acaaggcagc tttaatatgg atgatattat taacggtatt tgccggaaaa ttatacagcg
     2581 acacactcat gtttttggta aagataaagc agatacacca gaacaagtgg ttaataactg
     2641 ggaaataata aagaagaaag aaaagggaat gaatacccaa actgaagttt tgaaagcagt
     2701 accgaaaaat ttacctgcac ttatgcgaag ttataaggtc cagcaaaagg cagctcagtc
     2761 aggttttgac tgggatgaca tagaagatgt atttattaag gtttatgagg aaattcagga
     2821 gctgaaagat gtatataaga gtaaaaatgt ggcaagaata aatgatgaaa tgggagatat
     2881 attttttgca actgtaaatt tagccaggtt tttacatgta cagccggagc ttgctctgac
     2941 acatacaact aataagttta ttaaaagatt tgagtatatc gaagaagaaa gcattaaagc
     3001 tggtaagaaa ctggaagaaa tgactctgga agaaatggat aagctatgga acgaagcaaa
     3061 aaagaatctt cgggaagatt aaatacttaa ttatgcaaat acctgaatac aaattgctta
     3121 aatgctattt aagcagtcaa aaaaaccaaa ttaaaggagg gtaaaaaatt gaataaagca
     3181 gaattgatat caagtattgc tgagaaaacg gggatgtcta aaaaagatgc ggaaaaggct
     3241 ttgaatgctt ttactgagag tattgaagat gcccttgaga aaggtgaaaa ggttcaactc
     3301 ataggttttg gatcttttga agtaagagag agagctgcca gaaaaggtat aaatcctcag
     3361 acaaaagaag aaataactat agaagcgtca aaggtgccgg tttttagagt tggtaaagct
     3421 ttaaaagata aaattaataa tatatagaag ctggcaaagc aggttttttg acataactca
     3481 aagcgttgga aacacatgac atatgttagg ataagcctgt catatgccaa caccaaggat
     3541 tgtgatttcc aacgctttaa ccaatactat atatgtagcg ttggaaacac atggaaaggg
     3601 gattttaaag gtgaggattg ataagttttt aaaactttca agggttataa aaagacgtac
     3661 ccttgctaat gaagcttgcg aatcagggaa agttaaaatt aatggacgtg ttgtaaaacc
     3721 ctctgctgaa gtaaagcctg gtgatataat agaaattaca tttggtagta aaaccagtaa
     3781 aatggaggtt gttacaattg ccgagcatgt tccaaaatca ggagctgcag aactaatacg
     3841 aataattgaa taaatagttg ttgaatatat atttgatctc actgcagaaa ttcatgtacc
     3901 aacattggac ttttgcagtg tagcatagtt attgcagaag aatcatattt atacgatttt
     3961 gacataataa aaaataatcc tttagttgct aaactacctg gattattttt tattgtgctt
     4021 ttatgcatat atgattttgt acatgcatat aaatttattg aatctacata aggttataca
     4081 gaataaggtt aaatatgata tatatagttc ctgacacgag ccttgcagct gtgtacaaga
     4141 actattatac aaggaggtta aaatggcaga ggataagaag ataattaagc ccaaaataca
     4201 aaatttaatt cttgagaaca gacaaaaatt aagtgtatct ggagttattg atgttgaaag
     4261 ttttaatgag gaatgcgttg ttgttgatac agaaatggga attttaattg ttcatggaga
     4321 ggacctacgt atcaataaat taaatcttga taattccgaa ctaaatattg aaggtgatat
     4381 tataagctgc gagtatagcg ataaagaagg accaagatct aaaggtattg gccttttcag
     4441 cagaatgttc aaataaggct ggtgaatata tttgccaata tcaaccagta tacaggcaca
     4501 catttttcta tactccatat tatgtggtat ggttatagga tttgtttatg atgtttttag
     4561 aataaaaaga aaaacaatta aaacaactgt cctatttata tatattgaag atattctcta
     4621 ttggattatt gtaatggcaa taatgtttgc ccttatattt tacagtaatg atggtgaaat
     4681 aagagggttc atttttattg gagcattaat aggtgttata ctatatatgc tcctgctgag
     4741 taaaattata gtaaaatttt ctttagcaat attgaatatt ttaggaaaga taataaaagt
     4801 tattttgttc atagttgcat atccttttag actaatatat agagtattat ctattcctgt
     4861 aaaattactt atgaaacctg caaaaaaatt caccaggtct atcaaggcat attctagaat
     4921 taaaatggta aaaatatcta tgtggaaaag atccttaagg aatagaatta aaaagatatg
     4981 aacaaattaa agataaaatt aaaattaaaa ttaaagttaa aaataaagat aaaattaaat
     5041 taaaaaaagt tacaaataaa gaggattttt tagattaata tagaattata tatatatgca
     5101 tcgcaactat gctgcaaaac aaaagaagtt ttataataac tgcggtgaat taaggaacag
     5161 tggaggatca gatttaatat gaacaaaaga aaaggttcga tatttggcat attagttctt
     5221 attgcattga tcatatattt ctcttttatt attatcgatc aacagaaaat gttgaaaatt
     5281 aaagaaactg cactgatgga aatagagaac aagataaaga aggaggaatt agtaagaaat
     5341 gatcttgaaa aacaaaagga tatgcttaat agccgcgaat atattgaaaa agtagctaga
     5401 gaaaaactag gtatggttaa accgggtgaa aaaattttta ttgacataaa taagtaatca
     5461 ataataatta tggtaattgc gcttaataat acttgactac aaactggtaa tactatataa
     5521 ttctaattac tttagattat aaaaaagtga aaataagagt acaatgaatg actaattttt
     5581 tgactgtgga tctatggaat aataataaaa taaataatta tataaataac aatataagca
     5641 tatggtaact gtattacagt tacataaatt ttaaggagga acaattattt tatgctagtt
     5701 gaggtaggca aggtaattga tggaaaggtt accgggttaa caaattttgg tgcctttgtc
     5761 cggttatctg atggaaaggt tggtttggtc cacatttctg aaattgcaga tgagtatgtt
     5821 aaggacgtaa attcatatct taaggaaaat cagatagtca gagtaaaaat tatttctgta
     5881 gatgaaaagg ggaaaattag cttatcgata aaaaaagcta atgatggaac taagaaggtt
     5941 aaatcaagca ggcctgaaga gatagactgg agtaaatctg atgggaaaga aaaaatgtct
     6001 tttgaagacc agcttgcaaa gttcatgaaa gacagcgatg agaaattgca ggatctaaag
     6061 aaaaatttcg aatctaaacg gggaggcgga ggatacaaga aatacgccaa ttattaatct
     6121 atttatccat ctagtttttg tttatctgtt tagtatttat tatataagtt acattctata
     6181 cagctttatt ttataatacc agcaaaaact ttccaaaaaa aaactacttg acacgcaaat
     6241 gtattagtgg taatataata aagcgatgtg ccggagtggc ggaactggca gacgcacagg
     6301 acttaaaatc ctgcggttcg tgaggactgt accggttcga ttccggtctc cggcaccact
     6361 aatttaatat cgcggggtgg agcagtacgg tagctcgtcg ggctcataac ccgaaggtcg
     6421 atggttcaaa tccgtccccc gcaaccaaaa aaacaaagca ttgcaaagag atttgcaatg
     6481 ctttaatttt ttgctctttt caaaggaatc tttattactg aatcccggtt tttgtggtat
     6541 aatcttaaat atggttgaaa tgaggtgcat atgagttgca taaatgtaag catacaatgt
     6601 atgcattcaa taagtaagta aggggtggca gtgtgaaaat aaaggtaatt ggttcaacca
     6661 aaagcggtta ttgcatgccg gttgaagatg cattggggtt cggtggtaaa tcagcgggta
     6721 tatgttatat gcctgatagc ttcgaatcca tattgggaga gaatattgaa aaaaccgaaa
     6781 aacggattca acgcacatta aaatcgggtc atcacagcgt atatgatcat cctacatata
     6841 atcttttact tgaagatata cccaaaatat tagctatggt tttaaataat gagggttttt
     6901 atactacatc tgaaaaatct gccaggtata ctaaaatgaa tcctggtaaa agggaaaaag
     6961 acctatacga aaaatgggct gatatcctaa aagaaaagat tcatgaaata tatccccaaa
     7021 tatctaattc tcatactgaa aaactggcca gagaaaattc cagatacatg attagtgtat
     7081 tcactcccac aactttggaa tatacagtga gcctaaggca aatgagctat attttgggta
     7141 tgatgaagaa atttgttgag aaagctgaag ataccagttt caatacaatg cttaaagaaa
     7201 gcattggaga atttatagaa tcaagtaagg aatttgatat accctattta aatgctgata
     7261 ttaaaggcag ggagctttcg ctttttgacg cccggaaaaa taggcgggac gaatttgggg
     7321 agaattactc tgtcaactat ttgggtagtt ttgcacagct gggccaggcc caaaggcata
     7381 gaacactgga ctataaaatg aaagtaattg aggaaaatcc tgaattctat gttcccccaa
     7441 ttatacgagc agaaaacaat aatggagaca cagcccttga aaacacatgg ctgcaagata
     7501 ttaaatctgt tgcggatatg ttcccgcagg gcatgttgat taaaataaat gaaagaggta
     7561 catacgaaaa ctttatcctt aaggcaaaag aaagattatg cggaagtgca cagcttgaaa
     7621 ttgcagagca gactaaagac atacttttag catacatttt gaatacaagg ggaacaaatg
     7681 atgaagtaag agagtatctc gcacaatatg ttataaaaaa atatgctgct aatgcaggaa
     7741 acgttgatgg gatggaatgt gatggaaata cttttgccgc tgacattgct gaaaatactc
     7801 atggatatat agcaataccc cgatgcagat ttcacgactg gaaatgcaca agtccatgca
     7861 tatggggcgc aaaaggattg gaaagactta tatgataagc tctgcagcaa tttcaccaag
     7921 ttgcagagcc ttggttatta tatttgatac ttcatcctgt attctccggg ggttatcgag
     7981 gtgtatttct tgaaagatct gatatagctg aagcggttat tatagccaac catttcagct
     8041 atctgttcta tactattgtc tgtttcaatc agaagggact tggatttctc tatccttact
     8101 tttgatacgt actcccagaa gcttacccct gttttctctt taaatatttt acaaaaatag
     8161 gaagaattat agttgaaatg tgcggctgct gtaacaagag atatttcttc aacataattt
     8221 tcttcgataa actccttaat ttctgaaaca aagctttttg tattatcttt ttgctgatca
     8281 atctggtatt caataattgt tctgaacttg gagcttatcc attccataaa ctgctgaatg
     8341 cttttaaatt tgtcaataga aaaggcaggg atatcgtcct tcccgaaaac ttcatgtata
     8401 ttaagattga gatcaaatat gcacctttca ataattcccg atatgtttga taaacatatt
     8461 tcaatatgct gaaaactctt gttgtggtcc attatatcgt taatcatgtc attgaggata
     8521 agtatggttt tatcataatc acaaagcttt atcaatgaaa taatcttttg ctccatttca
     8581 attggataat aaagcttttc ctttttactt cccataatat ctgtataact gattacggac
     8641 tcgtcaccct taagaaattt gtgattcagt gcttcgatag cttccttata agatacaccg
     8701 atatttgaaa tatcattata cattcttcct atgccaatgc ttacaggaat gcttaaaaat
     8761 ttttccgtgc tgtctttaat atcttccagg gcttgctcaa taattgcttc cacttcgttt
     8821 attttctcaa cagaataaag ctttagtaaa attaaaacat tatcatctga gtcctgagag
     8881 cacacaactt caatatcaag gtcgttgaac actttatcaa caatattaat taaagatatc
     8941 tttttaaatt ccagatcgtc tatgctaatg taattggaac ttacacaacc aatcttaata
     9001 actgcaacct taaagaaatc aaagtcaaga ttgagcataa aatactctgc tctattctta
     9061 atttcatttc tgttaaaaag ttttcccgag ataagctgac tgagaaatat ctcacgtacc
     9121 aaaaccttat tgtcatccaa ctgtttttca agagtttgat tgttttcaag aagcattttt
     9181 atactatcct gaatcattaa aacatcactt aaattgtcat catttactgt catcatcttc
     9241 tttatattta aaattatttt ctttatagga ttgtaaagcc tgaaggaaaa tgccagtgaa
     9301 acagcagtca taagcagaag cagaatggca agtataatta tagtcatata ttttatatag
     9361 gcagatcttt cataaatctt gttttcagag actatataaa agagcttcca gccattaatt
     9421 cttgaagtct caaaatagga tatcattttt gtttcctgca ttttaaaatc aaagcttccg
     9481 ttatttaaat ccatgtatcc ggaagggata ttgatgatgt tactgatgtt ttcatatata
     9541 taattatctt tatttgatga tatgaccata ccgtctttgt taactaaaaa tgcaagggta
     9601 tggtcatcac cgtatgtgat atttttcaac aggtcggaaa taattttttc atctacacta
     9661 aggatgattg caccttcctt tgattcatta tcatagggta ttcttgtaat aaatgtaatt
     9721 acattattgt gggaataatc tgatgacctg tttctatcgt taacttttcg tgtgttcatt
     9781 attaatgaag aagccatcat tttacaataa tcatcatacc atcctttatc ataataggac
     9841 tctatgccca ttggtccgct gtaatccaat acatagtcgt ttttttcata ataaattgct
     9901 atatttctga tataattact agagtttata gagtttgaaa gaagttcctg gatgcttttt
     9961 acttccaact gcttttctgc tataacatca cgtggaagat aggcaaattg caaaaatttt
    10021 gaattcatag catggtttag cgaaagtttt tgcatttcac caaataaaac attcatagtg
    10081 tttattgttt cgttgatttt tttccgttca aatttttcta tttcactatc caggtttttc
    10141 agtatataaa agcgggtgta tgatcctatg ataataacca gaaataaaga tataagggta
    10201 catgccagaa agaattttat aaacaggctt tttgaaacgc ttttggtata cttctcgaaa
    10261 aacttggtga tgctcatgtg tgaaagctcc cttatataaa gtcttttgca ggggaacaac
    10321 ctgctgtttt aattatagct taccatagaa acagtgtggt tacaatacaa agataaaaaa
    10381 atgaatattt gcaacagaag gaaataaatg caacatttgg ttgtttggga agggtgtttg
    10441 acaagaaatt ctgctagtgt tgcaaaaatt cagattttca ggtaaaggtc cggatatgca
    10501 acaaattgta taaaattgct acttgcaacc tggaggtaca gtaatataat gaaattgtca
    10561 gacaaaaagt tgatttatta aaatcataag taattaaaac ccaaaattaa aggagggtgt
    10621 ttcaaaatga caggaaatcc aaaaaaagtg gttgcattag tattatcatt atgcattatt
    10681 ctatcacttg gatttacagg gtgtgtgaaa aaaaccgtaa gtgaaggatc aaaggaacct
    10741 tctgctcaga caactgctaa taatgccaac actcaggcaa agccggaaga accttatgag
    10801 tttagtattt tcaggtctac atggacagaa ctaaatgaag atacagacct ggtcatacag
    10861 gaattaaaca agaagtttaa catcaaaata aaaatattga catgtcctta tgaggcttgg
    10921 gtcgaaaaat acaatatata tgtaacttcg ggtgatgtac cggatttgtc ggtaacaaca
    10981 gggccgggga ccatgaactt taatgcatgg gcaaaacagg gcatatacct tgaactaact
    11041 gatctttatt caaagcattg tccaaacatt caaaagcata ttagcaacga cataatagaa
    11101 gcccatagaa ttaatggtgg attgtatgga attccaaaac catcgttgtc tgatacaaca
    11161 gctgctatta gagcggattg gctggagaaa ttaaatcttt caatgccaaa aactcttgat
    11221 gatttatatt cagtattgaa ggcctttatg gaaaatgatc ctgatggtaa taataagaat
    11281 gacacttatg gattgtgttc agaggacact ttgaatacca ttgattttat ttttggagct
    11341 tttggctgtc actgcccgcc gaacatagct acaaattggg ttccggatgg aaatggcaaa
    11401 cttacatcca atttattgga acctggtatg aaagatgcaa ttaaatatgt tacaaaactg
    11461 tatcgtgata aaattcttga ccaggaatgg atgctgacaa aatctcaggc atacgttgat
    11521 aagattcata caggcaaggt tggggttaca aaagtagcat tccaaacaat gatagctgaa
    11581 acggaaacta agataaaagc taatgatccc gcttgtaaac ttgaaatttt agaaggagta
    11641 actgggcctg aaggtaaata tgcacgtccc atgcaaaaag gtttttacat ggtatcatct
    11701 atttccaata aagccaaaaa tcctgtaaaa atattgcaat tccttgatta tttaatgtct
    11761 actgaaggtg ataagttaat aagatatggt gttgaaggta ttacttatac agagaaagat
    11821 ggaaagattg taaggaatga agaagctgtt aagaaatatg gcatggaaag cggacataag
    11881 ttcagacaga tattgcagtc catagatatt gttatacccg gggaagaccc gagaatcccg
    11941 caacttaagg aaatggcaaa agtattgtat gaaggaccgt tctaccctgc acctaccctt
    12001 cagcctgcgt ctctgaaaga agttactaca aaacaggggc cagattttgt aaaaaattca
    12061 gtagcagcta ttgttaccgg caatggggac gttgataagg aatgggatga gtttattaag
    12121 aaatggagag aaagtggcgg agaccagctt ataaaggaaa taaatgagtt atatgaagct
    12181 aacaaataga aataagcata ataattataa attatacaaa gaaggatatt agtaaaggaa
    12241 tacgtgtagg tcaaatatca agtggggaat tgaagtgaga tgtatctccc cggtagtatg
    12301 gtactatgga caattatgta tgctatcggg gagatttggt tttaaacgtg gataggatgt
    12361 aacgactata taaaccaatt cacataaaca aatttataga aaatgtttta tagaaaccgt
    12421 aatttgaaaa tgcagggggg aagagaaaga gatgacacaa acaacgaaaa acggaaatca
    12481 agccagaaat caagtctgga ataagattcc gaacaaggct cagaagcata ctttgaagca
    12541 aaattggaat cggagcggaa tggattccgg aaaaagcttg ctaaaatata tatggagaaa
    12601 aaagtttatg tactcaatgc tgcttcttcc tctcttatat tatataatat ttgagtatat
    12661 cccaatatac ggaataatga tagcatttca ggattatgca cctggaaaac cttttatagg
    12721 cagtgagtgg ataggactaa aaaatttcag attggttttt agctcccaac catttatgga
    12781 atccctaagg aatactatac taataagctt ttataaaatc atttttgggt tcccttttcc
    12841 gataataatt gcatttcttt taaatgaagt aagatctgtt ccttttaaaa gaacaattca
    12901 aacagcaatt tattttccgc actttgtttc atgggttatt gtgggagcga taattgtaag
    12961 tatcttttca ataaacgatg gacttttgaa ccaaatattt agacatatgg ggattgcgcc
    13021 ggtaaatatc atgtccaatc caaaatactt tagaaccata ttggttgtgt catccatgtg
    13081 gaaggaagcc ggaatgcagt caattattta cctggctgca atagccggag tagatgttca
    13141 ggtatatgaa gctgcaataa ttgatggagc aaacaaatgg cagcagacaa ggtatgttac
    13201 acttccggga atagcaagta caattgcaat tttattcgta ctcaggttgg ggagcatttt
    13261 aagtgcagga atggagcaga ccatggtgct tttgaatcca atggtggcaa gtgttggtga
    13321 aaacattgat acctatgttt ataaggtcgg tttggggcag ggagactata gtttctctac
    13381 tgcagtagga ttgttcaaat cattagttgg atatgttttg attatgggct caaattggct
    13441 ttcaaagaaa atgagcggcg aaagtatatt ctaaaaggaa acttaactta attaggagct
    13501 gaattagcat gagtaaaaaa attaatctgt ttaatgtaat taactataca ctgttattct
    13561 tatttgcctt gtcggccatt tatcctttta tatatatatt aacaatatca ctactgccag
    13621 tggatgaata tttgaaaggc gggccgttcc tgttcccgag gaatatttca tttgaggcct
    13681 atatgcatat tttcaaaaac ggaaatattt tcctatcatt taaatcatca gttattatta
    13741 ctttacttgg aacctttacc aacctatttt tgactatttt atgtgcatat gtaatgtcaa
    13801 aaaccaaact aaagggatat aaacttttta taaatattat tattttcacc atgtacttta
    13861 aaggaggatt gattccttct tatatggtga tgagaagtct tggacttgta gatagttatt
    13921 gggctgttgt gcttactggt ggaataagca cctataatat gcttgtacta cgcagctttt
    13981 ttattaatat acctgagagc cttgaagaag cggcaattat agatggcgca agcgaacctt
    14041 acgtactttt aagaataata cttccaatat cactacccgc tattgcaact atcggtttat
    14101 tctatgctgt agggcactgg aactcatatt tcacaccttt gatatattta aacagccgca
    14161 agaaatggcc tttgcaggtt atactgagag aaatcctatt tgaatctaca gagtctatga
    14221 ggactgccgg tgaatctaca gagttgcaga agtctgcaat aggacctcag ctaaaaatgg
    14281 caacagtaat tgttacaata atacccataa ccttgatcta tcccttcctt caaaagcatt
    14341 ttgtcaaggg tgtattgata ggtgcagtaa aggcataaat ataaatataa atataaattc
    14401 aatgtttgtc ctgatgcatt ttatattgta taatactgta taatacattt atatgaattc
    14461 atttatagag gtgactgctg tgaaagccat ataattgtat tgaaatgaat tgctgaagag
    14521 gagcggacta atgccctatt tatatgagga cacactgcga aaaaaagcgg aagaatatat
    14581 cctggtattg aagagagccg gtattgatag cgaagtttta atgttacggg attacatggc
    14641 aaagctttca ataaagcggg aagaaaagaa ttacggaaat ttgatattag actataaagc
    14701 taaaaaagat actttttcaa cacggacaca ggaactgcag gacaaatcta tagaagatga
    14761 actgcttatt ttatgggata ggtgtaaatt aacggctgaa cctataaaaa cagcacagtc
    14821 tgaagaagat agagaaaaca gattagacag ggaagacaga gaagatataa aagatggaga
    14881 agatagagaa ggtagagaag atagagaaga tagagaagat agagaagacg gagaagacta
    14941 ctatgaaata tatgtggatg gttcatatat taataattgt gtaggatatg gggtagttat
    15001 cctgaaaaat ggacagcttt taaaggaatt atccggtctt gttattgatt cagtttttac
    15061 aggttcaagg caggtggggg gagaaatcac tgctgttatg aaagctttgt cctggtgtaa
    15121 gaataataag atagacaaag tatcagtatt ctatgatctg gaaaacttga agaaatgggt
    15181 tacaggagag ttttcaacca atattccaat gtcaaaggct tataaggatt ttgtgcaaaa
    15241 cagcggtata tgcattcact ggaataaagt accggctcat actggagtga agtggaatga
    15301 atgggctgat gaactagcaa aaaaagggac gaaagaatgc acagcagaag gtatagcaga
    15361 attcaaaatg aaattcaaaa cagaatgtga aacagaatgt gaaacagaat gcacgccaga
    15421 acgtacaata gaacatataa cagaatgcgc cacaaccaat cctgatagtt gcggcgatct
    15481 tattgaggaa ctggaaaaaa cagcagaggc atttacacta tatctggagg acaataacta
    15541 tattgctgaa tttctgcgta tttataacaa catggctgct aaaatctgtg tagaaaagga
    15601 tggggagaca ctgggatata ttgatatata caataccagg aaacttcatc ttcaacctaa
    15661 atatcatgag ataaggcagc cggaaatcag ggaagagatg gaaaaacggt ggaaagcctt
    15721 ctctcaaaat aagcagatat aataaaacta ttgagcttca catcctgtcc tcatagtatt
    15781 catcgggtat acatggtaat ataatagtga atgttgttcc tttccccaat gtactttcta
    15841 cctggattct gccattatga tccttaatta tattatagca ggtacttaat ccaagcccgg
    15901 taccgtttct cttggtagta aaaaaaggtg tgccgatttt ctgtaattcc tcttcagata
    15961 tgcccttgcc attgtcagat attttaatgt acatcttgtt tgcttgctta tcatatcctg
    16021 tgcctacttt cagtatggca aggttattac tattgctatc cattgcatca actgcatttt
    16081 tgcagatatt tagaatcact tgctttattt gagcttcatc gcacataaga tatctttctt
    16141 catctgataa atcaaattca atattaaccc cttttatcag tgatgatgtt tcaatcatgc
    16201 tttttaagga atgtataata tcatacattg atacttctgc aaattcaggt tctctgggtt
    16261 ttgataaata tagaaattca ctcattatat aatttagctc atcaacatta ctgctgattt
    16321 tagagatatg attttttata tattcatttt caacgagatt ctcaatgatt tggcagcggc
    16381 cttttattgt tgaaagaaaa ttacgtgatt catgtactat acttgcaccc atttgaccca
    16441 ataatgcaag cttttcctgc tgtaaaagga tttgctgctg ttctttcatt gctgtgatat
    16501 cagtacctac acttattcca cctataatct cttcatcaat attgaaaatt ggcgctaaat
    16561 acaataaaaa atttcttata gaaccgttag aagatgtgaa tgatgtctca tgatattgat
    16621 ttgtagtatg accttgaaga accagattca gcagctcttt agagtcagaa gtcattaact
    16681 tgtcgaaatt atctatatcc atacctatta tatttttgct atccaaccca tataattcct
    16741 cgagtgcttt attgcataaa acaaattttt tgtttttgtc taccattaat atgcagttgg
    16801 taactgcgtc tagtatagtc tgagtttgca gatgtatgtt cttaagttcc tgttcctttt
    16861 ttgcatcgtg aaagattttt aaaaagccat ccgttatttt atgaagggaa acattaagct
    16921 tgatattatc acctgctgat gttcgatatg ccctgattac attacttata gtgtttccat
    16981 tagaatcttc attttgcagt ataataactc ccggactatt ttcataagca ctactgactg
    17041 caaatttctc aagtagctgt ttttcagtgt gaccgtttag gtcattccta ttataaccca
    17101 acagttcttc aaattttcta ttagcatgtt ttagtattcc attttgatca tatctgataa
    17161 tagcttcagg aagtgcatca agtatatcat ttaaagattc ggtcaggctg gtggactttc
    17221 taagggtttc ttccagtttc ttgtgtgcat tctcaagttt tgtatagggg tagaatacag
    17281 tacttgcaaa aatgctttta aaaaggtaaa agcaggatac aatttttagg atatggccaa
    17341 tagtacatgt aactgaagtt atagaaatat aaaatataaa gaataattct gctgagatag
    17401 acaaaatcaa tgaaattaaa atatatttgt ttgataatgt atgttgctga ttatgatctt
    17461 catcagaaac atgattgcca actaaatgtt ttgagttata taaggcaagc agataaattg
    17521 ccactataat acattcaaat atggttttag ttggtgttac acctcgctct gtcaacaata
    17581 taatagattt atcagtataa gagtttgtaa agtatagtat acataatgta aaaaataaat
    17641 caacaaataa aataatccat ttatttaaat ttaaaaaaaa tgttaatata tttatatatt
    17701 tcaagtgatt tgatgttttt ttgaactctg atgtatataa ttttacatat agcagcagga
    17761 ctatagcttc tgtaattctc cctaataacc aatactttgt agacaggtca aaatatgtac
    17821 ttgagcataa gttcagtttt agaaagtaaa atacgtggaa aatgtcaaag atagctaccc
    17881 taaggtaacc aaagcctagt attcggttat agaaagctgt attgctgcag gtgaaccata
    17941 aagaaaaaaa tgatgataag gcaatgaata cacgggctag ttccagtatt gtatggtata
    18001 tattcaaata tttaataaaa ctattatata aaaataatgc aaatacaaca gttatggtta
    18061 taaccaaaat gctttgcagc aatatatttt tcattgtatt atttttgctt tgatatatat
    18121 acattcccat ttaagcctcc atttctgcta taactaattt tatataaaaa ttcaaagatt
    18181 tacaatcgta tagtaaaaag atttatagaa gacggaaaaa taaactgtta tatagatatt
    18241 gttaaaattt aaacaaatat gacagtgtat gatacttttt agatctttgc ttttgaataa
    18301 atgtatgtta atatattaac aaagtttacg aaaagtttat ttggcgaaac attacttatg
    18361 ggaaaataag tatataatac atataatatg tattatgaga ttgggaggca ttatattatg
    18421 gcaataaata aatcaaaggt agctattatt ggcgctggtt ttgttggtgc atcaacagct
    18481 ttcgaaatgg cactgaatca aaatgttaat gaattggttc ttattgatat caataaggaa
    18541 aaagcacttg gcgaggcaat ggatataaat catggccttt catttattgg acagatatct
    18601 gtaactgcag gggattattc ggactgtgct aactgtgatg taattataat tacagcaggc
    18661 ctaaatagaa aacccggaga atcccgcctg gatcttgcaa aaaagaattt agcagttgca
    18721 aaagatatta ctgataatat tatgaagtat tataatcatg gtgttatact tgtagtttct
    18781 aacccggttg atattcttac ctatatggtc caaaaatggt caggacttcc aaacggaaga
    18841 gttatgggaa ccggtacagt tcttgacagc tcaagattta gatatctgct gagcaagagg
    18901 tttaatgtgg atgtaagaaa tgtgcatggt tatataatag gagagcatgg cgactcacag
    18961 ctccctgcat ggagtgcaac acatattgcc ggccggaata tagacacata tagtgcaata
    19021 ccggagaatg gttttacaga agctgataaa ttggcaattg cagaggaagt aaaaaaagca
    19081 ggaattgaaa taataaaaaa caagggtgca acctattatg ccatagcaat taccgttaat
    19141 actatcgttg agacaatcct gaaaaaccaa aatactataa gaactgtaag cagtatcatt
    19201 aacggttatt acgggataaa taatgtagcc atcagtctcc cgtcaatagt aaactctaat
    19261 ggaattaaag aaatatttga gatgagctta acaaaacaag aggaagctga tttaatgaat
    19321 tccgttaaac atgtaaaatc agttttagat aaagttaaga atatttatta gtactactat
    19381 accaatttgg aaagccatgt cttccgggat aattttgata gcatcgggaa ggtgtattcc
    19441 ttacgggtca acaaaaatta ccctccagaa atgcgttttt cattggcagc ataacaaagt
    19501 agtattagta cagttttgca atataatatg tgtgtttgca tatttatttg ggacacttct
    19561 caatgtaaat gaaatgcccg gtgcttgagg gagaagtgtc ccctgacctg accttattcc
    19621 agccgcttgc tgccagctaa caatttaatc agaacaggtc ctcataaata ttttcgagag
    19681 agtatttgct ttctggtccg gatttccggc ctatccactt accattggtg aaatcaggga
    19741 aaccaacggg gctgcttccc atggtaatag attgctcaga tagggcagta actgccatcc
    19801 atgctgctgt atcataaaca tcaataggag ttggcacttt attttttacg ctctctacaa
    19861 aggcgcgaag aaccaggtaa tccatgcctc catgacctcc acgaactcca agatcaagat
    19921 attccttcca gagaggatgc tcatactcat tcctgtaatc ttcaaaagat tcccatgtat
    19981 gttcaggact tctaccttct atataaatag agttattatc ttccatccag ataccttttg
    20041 ttccctgaac tcttccggct cttgagtagg gacgcggcag tgatgtatca tgaataataa
    20101 gaatggtttc accatgagca cactttatca ttgtagtaac tacatcacct aatgcaaaat
    20161 tggtttttgc aaaaggatgg tcttcaccct tgtttgcttt aacccattcg ttgataccgc
    20221 gtgactttga tgacatggag gttaaattta tgaatctgtt acccctgtta atattcaaac
    20281 acttggctac aggcccgagt ccatgagtgg ggtatacatc accgtttcta ttaaggtaat
    20341 tgtggattct gtaatggcgg ttttctatcc cgcaagcaac ctcctgtctc aggtcatgtt
    20401 catatccaca ttgacaatga ataagctcac ctaagagatt ctttttaacc atgttaagca
    20461 ctgtcatttc gttccgtcca taacaacagt tttcaagcat catacaaggc atacctgttt
    20521 tttctgaagt ccttaccagt tcccagcatt cttccaggga tgaggcccca ccaacctccg
    20581 ttccaacata cttcccggct tccatagccg caagggcgat ttccaaatgg gttgtccagg
    20641 aagttgcaat tacaacaccc tcaatatcat cccgttctaa caacttccta taatctttat
    20701 atccttcagg tcttttacgg cctgcagctt caacttgctc tatacccgtt tgaagcctgt
    20761 catcgtagac ttcacaaaca gcgggaatct caacatcttc cattgctaag acggttccca
    20821 gaaggctttt cccacgacca ctaagaccaa taaaccccag tctaacctta ttttgactgc
    20881 tttcattcat tttacttcac ctctctcatt atttttaagg tgtattagca aatttgctca
    20941 aaagtatata atacttctta atatatttta gcaagttatg gttattatta caatatacga
    21001 ttttattagt tttctcaaaa aatgattagt agtaacagtt ttatataata cttcaaattt
    21061 cagaataatt aaaaaactga aaataatatc tatattcgta tattgaagtg cgagtagcca
    21121 gtatatatac tttactggat ctatttaaaa ctaatcctaa ctggaacgag ggaatactag
    21181 taaaaaatcg tgatggttcc cttcggacga attggtatgt tgcaagtgcg gatgcatgga
    21241 tccatacttt ctgtaatttg cttgaacttg atacaatatt tgatatccaa acatggataa
    21301 ccaatatact tattggtgtt gccgcatata attgtcaaaa aaacaactca agaattcaga
    21361 ggatagttga gcaaataaag gatgttgtta ataaaaggtt tgctgaagat ttatctgtca
    21421 gttcagttgt atctgaagtg ttccttttag cgggatatgc aaccagtctg tttaaaaagg
    21481 aaaccggtat aagcattatg aattttatta ttaaagttcg tattgatgca gcaaaaaaaa
    21541 tgcttcaaga tcccagctcc agagtaagtg aagtatcata tcgggtagga tatgaaaata
    21601 ttgcatactt cagtacgctt tttaagaata ttgtgggaaa gactccaaaa gagtataggg
    21661 acagttaccc ggatttatct ctggaaaata ttgaagtaat catgaaaata atgtatcata
    21721 ataataagta taaaaatgca aacagataaa taatagatgt agatgttgtt gataaaattt
    21781 gtataggtgt ataacggtgg aaaggcggtt ttcgtaaatg attgaacttt cgaatttaag
    21841 caaaaccttt aatggtacta taaaggctgt tgacaatgta agcatggtaa taaaggatgg
    21901 agagatattt ggtttccttg gtcctaatgg agcaggtaaa accacaacta taaaaattat
    21961 gacagggata ctgaatgccg atagtggaag cgttaaaatt aatggaattg atttaaaaga
    22021 tgatcccctt ggagcaaaag cccaatttgg ttttgtacct gatgatccta atatgttctt
    22081 aagactgaag ggcctggaat atttgaattt tatggcagat atctatgatg ttccttcaga
    22141 ggtcaggaaa gaaagaatag agaacctggg agaaagattt gaaatgacaa aagcactggg
    22201 tgaccagatt caaagttatt cccacggcat gaaacaaaag attgttatta tgggagttct
    22261 ggtgcacaat ccctcaattt ggatacttga tgagccaatg acaggccttg atccgagatc
    22321 ttcctatata ctcaaagaaa tgatgcgtga acatgcagat aacgggaaat cagtgttttt
    22381 ctctacacat atcctggagg ttgcggaaaa aatatgcgat agagttgcta ttataaataa
    22441 aggtaaaata ttattttgcg gaaatctcga ggaaatgagg gagcatttta agagtaatga
    22501 atcactggag aagatgttcc tggaactgac tgaaaatgag taacctactg aaaatgatca
    22561 attcactatc actgaaaaaa agtaaattat tattattgga gttgattgca aatgagcaga
    22621 tttatgataa ataaaagatt atttatactt acaagggtgc ttttgaaaaa tggcggcggt
    22681 gttcaagaaa agaaaaacaa gaataaaaaa agatggaaaa taaacaaggg attattgatg
    22741 tatatccttg taggacttgc ctttttacct ttggctgtta atataatatt tatggcagaa
    22801 gctatatatg attccctggc gcaaattcaa cagcagggta tagtactgag cctgggtatt
    22861 ggcatagtat cattcataat gttcttcttt ggaattttct atgtaatgaa tgtattttat
    22921 tttgcaaatg atattgaaag gcttctgcct cttccattaa aaccatctga gataatggca
    22981 gccaagtttt ttacagtggt gatttatgaa tacttaactg aagcagtagt aatgctgcct
    23041 gtattgggag tttatggagt aaaaagcggc gccggaatgc cctattatct attaggattt
    23101 ttaatattta ttttgatacc ggtaattcct ctctcctttg catctattat aataatgctg
    23161 ataatgagat ttaccaatat aggtcggaat aaagaccgct ttaaactaat aggcggcatt
    23221 gccgctgtat ttattagcat aggaattaat gctgcaattc aaagagcaac catgcaatcg
    23281 gtagatccca aggcacttca aaatatgctt atacaaggcg gtaattccct tgtggggctg
    23341 gctgggagaa tgttcccggg gaccagcttt gctgctgtag gacttgttaa cagctcaaat
    23401 attcaaggcc ttagtaatgt tctggttttt gtagcaataa cagttattgt atttttccta
    23461 tttatgtatc ttggagaatt gctgtatttt aaaggggtaa taggattatc agaagccgga
    23521 ggctccagaa agatagggat tgaggaatta aaaaggaaaa gtgtaaggac ttcaaagtta
    23581 aattcatata ttgctaaaga actaagaata ttatttagaa cacctatata ttttataaac
    23641 tgtgtactca tgaattttct ctggccggta ttttttatta taatgccatt atcccaatca
    23701 ggtcaatcgg gaggacccga agatatgaat atggaaatgc ttaaaagcct ggtgcaaaac
    23761 gatgaatatg caggtattat tcttgccata tcatttgcag tgatattgtt tattgcagga
    23821 agcaatggtg ttacatcaac aactatatcc cgggagggtc agcagcttta tataagcaag
    23881 tatttgcctt tgagttatgt tacgcaaata aaagcaaaag ttttatctgg agttgctatt
    23941 tctgctatag gtatgctggt aatgattctt attatgggtg ctgtgctggg gctacctctt
    24001 ctaattattg tcctttcttt actttctggt tttgcagcaa tattcctggt ttcatttaca
    24061 gggataataa tagatttaat taatccaaaa ctcaattggg ataatgagca gaaggctgtt
    24121 aagcagaatc tgaatgtttt gtttaacatg ctcatatcta taggcttagc cgtagctata
    24181 gtatttgtgt cgattaagtt taaacagtat atggatgttt atgcaacctt tgctttcctg
    24241 gttttaatat ttggacttat agatgttgta ttatataagt ttctgaatac caagggtgta
    24301 aagttgtttg cgagaattga agggtaaaga aaagcatatg gcgaaacaaa ttgcggatat
    24361 gaaacaaata ttacaacata taacatttat tacacaataa tatgaattta caagaactat
    24421 ttcgttgata taatagttta ggtaagataa tatacattct caaaacaaag aaaggccaaa
    24481 ttaatttatg tttatagaaa acgatattaa ggatagtgtt gataataaag taaatactgc
    24541 taataatgaa atctgcgata aaaccaacaa aaccaacacc ctaaacagag aatccaactt
    24601 aattatcaat catagtaata aattgagata ctatttaaaa aagtatatat ttagtgatag
    24661 accgttttat gagaagcttt ggatgttggc attgcctata attatccaaa atttcatgtc
    24721 ttccttttta aatataatag atactataat gattggacag ataggtgaaa tacaaatagc
    24781 ggctgttgga gttgcgaacc aatatttcct tctttgcctc cttgttataa ttggaataca
    24841 cagtgggtgt agtattttaa tgtcccagta ttggggtaaa ggagacaaga aaaatataat
    24901 gaaagttcag ggcgtaggct taatagtttc gttttttgct gcaacagtat ttgctactct
    24961 ggcttttgtt ttctctaaag aaattatcag catatttaat aaagaagaat tggttatagc
    25021 ggaaggtgcc aagtacctga aaattgtatg ttttagctac atctttacgg ctattagctt
    25081 aggttatgca tttgcttcaa gaagcatagg ggatgcttta atgcctatga ttgtaactgg
    25141 gattgctata atatgtaata ctacatttaa ttatgtttta attttcggga aacttggtgc
    25201 acctgctctt ggagttgaag gtgcagctat tgctacaaat attgcacgta ttgttgaatc
    25261 tattataata gtatcttaca tttatggtaa aaatagcatt ctggcagtta agtttattga
    25321 tttacatggt atccaaaggg aatatctcat taaaatgttt aaaactgttt tacctgttgt
    25381 tttgaatgaa ggattttggg cattaggttc tgttgttcga tctatagcat atggccgtat
    25441 cggatctcat gctatggcag ctgtgcagat atgtaatacg gtgcaaaacc tgtttaatgt
    25501 ggcagtattc ggaatggcag cggcatccgc agttatgatt gggcataaaa t
//
LOCUS       DAOSDD010000030        25368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_882_length_25368_cov_5.070083, whole genome shotgun sequence.
ACCESSION   DAOSDD010000030 DAOSDD010000000
VERSION     DAOSDD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 25368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25368
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_25368_cov_5.070083"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK733_04500"
     CDS             complement(<1..258)
                     /locus_tag="PK733_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ09835.1"
                     /translation="MLVRKIWDENEDVKRKMLVNREEAETIALGYVSVEKGNSTQVDT
                     HDDEEEIYVILKGRAILMIGDEKQEVGPGMIAYVPRNKKHNM"
     gene            complement(377..1144)
                     /locus_tag="PK733_04505"
     CDS             complement(377..1144)
                     /locus_tag="PK733_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOJ09836.1"
                     /translation="MSSIKEMQVDKLRVKIYETRQAMGEGAAKEAASKIKELISQKGE
                     VNVIFAAAPSQNEFLASLIEDKGIDWSKINAFHMDEYVGISKDAPQAFSNFLKDRIFS
                     KVSFKSVNYIDVSAKDAEKECERYSNLLKQYPADLVCMGIGENGHIAFNDPPVADFND
                     KKVVKVVELEQKCRQQQVNDGCFKTIDDVPKYALTLTIPTLMACKYVFCMVPGKTKTE
                     AVTRTVNDEISTACPATILRTHDNAVLYVDKDSGSEL"
     gene            complement(1284..2324)
                     /locus_tag="PK733_04510"
     CDS             complement(1284..2324)
                     /locus_tag="PK733_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ09837.1"
                     /translation="MHNKKTTIYDIAKEAGVSTATVSRVLSKSGYPVKEQTRKKILDA
                     VETLNYEPNIIGRMLKKNDSMDIGVIIPTISNPFYPEMVLGVEQEARLKGFNNLLCNS
                     FRDAATERKYIETLYQKQVKGIIISSIGENYKFLEIMQEKGIKIVTIDQDIEGMKCSK
                     VGFDYIKGGLIATEYLIEQGHNNITFVTSPLNRKSRRDAFEGYKLGLSKNGIPFNENN
                     VIVSEKEEEYENATYEFENGRYLAKRFMQIRNGSTAIFAVNDMTAFGIIQELLGNGVK
                     VPEEVSVVGFDNIEVSSMVSPPLTTVNQPAFETGKLACRILLHSLEGNGYEDTSIILE
                     PSLVIRSSVAKI"
     gene            complement(2633..3715)
                     /locus_tag="PK733_04515"
     CDS             complement(2633..3715)
                     /locus_tag="PK733_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09838.1"
                     /translation="MKKLAMIGCGGIGGYHLSHFLNFQDIELAGFCDLIPERAESFVS
                     KAGGGKAFTNYKEMYDAINPDMVFICIPPTEHGEIEFETINRRIPFFVEKPVALCMDL
                     ARQIRDEVKKAGLITAAGFQCRYSNLVGPNVKFIKENEIIFVDCIRIGGVPGVPWWRK
                     RSTSGGQIVEQTIHQFDIIRYVFDEPDMVFTMGTKGFVTDFEDYDTEDLSTTVVRFKS
                     GALAAISTGCYATGGNAADSKIVFSSRDARAELRILSDLKIFGYKDNNDIGNNGADAD
                     SKSDKSKGFVIKGDGALSVSKAGEITYKQEGSAGILCDRTFIDAVLTGDSSKIRSPYE
                     DAVRTLEFTLACNRSMDTGLPIKIQG"
     gene            complement(3808..4650)
                     /locus_tag="PK733_04520"
     CDS             complement(3808..4650)
                     /locus_tag="PK733_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ09839.1"
                     /translation="MKKAINAWSVPSHITFEEMFRDISNAGFDGIELNLDADNYSSHS
                     LTMSSGKEIFTEINHLAEEYNLPIASISTSLSGGKLGSDSKDVRESGKDIIRKQMECA
                     AELGADGVLTVPGGMTDSISLLQAYENSFMAIEELKSEIESAKINVGLENVWNGFFIS
                     PFDMKNFIDRLDCPYIGAYFDVGNVIAFSNPEYWIEILSTCIRKIHVKDFKRSNGINR
                     GGCFVNLLEGDANWSKVIPALRKTGYNGYLTAELGIIKDCPEYLYKITSDALDIIINI
                     SSSR"
     gene            4956..5813
                     /locus_tag="PK733_04525"
     CDS             4956..5813
                     /locus_tag="PK733_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09840.1"
                     /translation="MENQSYYRIWENSGCNEEINDHYLVVNCAGCCVLPHPFATHNKS
                     GRKDYYLLYICRGMMKVLVNGEMMDMCAGQFIIFPPECEYKYTRLGDEEVIYYWAHFT
                     GYGARGLLENCKLYGKNIFNAGFNEQVIREFNNMFSEFIRRDYCFEAAAASHLISACI
                     NLSRSMTNLATSSDAVNPDRIYASIKFIHKNFNSNISLKQLAGIEHLSISRYRTLFKQ
                     YTGFSPKNYIIALRINRACELTVQTDLSLKEIAESVGYHDQLYFSRIFKARTGIAPNC
                     YKKNYQNNA"
     gene            complement(5983..6933)
                     /locus_tag="PK733_04530"
     CDS             complement(5983..6933)
                     /locus_tag="PK733_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09841.1"
                     /translation="MNFLDTVKGSLLEGFFPAGWDMKKIDECCSNPPEEITKRQTFWN
                     EDFAPVPCDNIYDFNVLMGHEIALEIKKAKDEGRKLILILPVGPMGMYRWAVYFLKEW
                     NVDCSHVYGFNMDEWSDSEGNTLESTNPGAFQNAMEGAFYGPLGNLTVPVSQRNFATK
                     DNLPTYGEKIAKLKAEGAKLITVFGIGRMFHIAFWEPHFAADFCCEEEWKKQTHRIAA
                     KLHPLTIEQNAITSFKSRTTLVPCRANTIGPGLFLQSDKIIGGCDGTLGRGMMWQGVS
                     LWVALRYGPNIWIPASYMPTLPGKLFFLKELAGPLEPECN"
     gene            complement(7067..8218)
                     /locus_tag="PK733_04535"
     CDS             complement(7067..8218)
                     /locus_tag="PK733_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09842.1"
                     /translation="MKKWSNEKMTERKRYIETLLFGNPDKIPLQPGVPRESTLAAWHK
                     QGLPEGVNYFDALMDILGIPNPPAPKKTYLTVDFKMIPQFEEKVLEHKDGHYIVQDWM
                     GAITEISDEFDYTYIRAAKDFVTRKWHKFPVQSREDWEEMKKRYNADSPERLTENLDE
                     ICGKLQDRDYVAGISFNGPFWQLREWLGFENLCIKFIEEPEFIHEMIEFWTEFISRLL
                     ARFINKMELDKVHISEDMAYKAHSMISPAMTREFLMPSYIRWVKEIKASGCSIIDMDS
                     DGYIGELIPIWIESGINACDPIEVAAHNDIVEYRRVFGKSMAYTGGIDKRAIAKGGKD
                     MFNEVMRVVPPLLKDGGFIPGCDHGVPPDISWPDFIEYTRLLAKLTGWL"
     gene            8608..9483
                     /locus_tag="PK733_04540"
     CDS             8608..9483
                     /locus_tag="PK733_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09843.1"
                     /translation="MPDLQDKVDFNNLYVSKTADFITDYRDSKVDQVLSLEHYHDSYE
                     LDFFIKADIQIFVKDRRYEISNGDVLFISEYDVHKVLYNIGTHYTRYVINFKKEFLID
                     LLRFFKIDNLLEKIQKNDYKKVRVSINRRNEFESQFKTLLALYDSSRENCNAETAVLI
                     KSYLVILLTRFNETLKNAKPICEQQKKDHFVSRIIDFIDSNYAGVINLDLLEKEFHLN
                     KYYISHVFKEITGFTVMEYIQNRRIIEAQILLKSTSKDITEICYDCGFNNVQHFYRVF
                     SKITNITPYNYRKRG"
     gene            complement(9627..10844)
                     /locus_tag="PK733_04545"
     CDS             complement(9627..10844)
                     /locus_tag="PK733_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09844.1"
                     /translation="MKKYVLVGASGRAFGMFAKPLIEGYKDVAQIVGIFDINRTRAEY
                     IRDHTDSSIPVFTDYDVMMKETKPDAAIITTVDRFHHEYIIKTLEYGCDAITEKPMTI
                     DEDKCNAILDAEKRTGKKVIVTFNYRFAPYVTRVKELIKEGAVGDVLSVDFEYLLDTS
                     HGADYFRRWHRKLENCGGLLVHKSTHHFDLVNWWIEEEPEEIYANGNLRFYGPTRENK
                     GERCSTCQYASTCEFAVKYHENPFMQDFYFKAEKEDGYFRDRCVFADEINIYDSMSVA
                     VKYNKGTLLTYSLIAFSPYEGWKISINGTNGRLEAAEYHSGHKVKEPNYQIQLFNRKG
                     EVVTYDVPKASGGHGGGDERLRNMIFRGGVSDPLNHFAGSWDGVKSIMIGICANKSIK
                     EKKMFRLKDLIRK"
     gene            11281..12132
                     /locus_tag="PK733_04550"
     CDS             11281..12132
                     /locus_tag="PK733_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09845.1"
                     /translation="MQVIYSPNTLDTDFYIEHTKIASGTSMKTEHYHDFYELYFYLGD
                     GMRYFIGNKTFYVKKYDLILIDKFTYHRTSYKEKGFKERLLIYLYDDVINIIGNEILK
                     QKIVNLFKKKKISFYSSFSKYMLDTLMNRVLPKYYNKSNPSISQLQARLVMLDLLLEI
                     AEMDENGQLIEDNSITTPQENRVSDIVSYINSNYSKQITLDELSKIFFINKYYLCHIF
                     KEVTGMSITDFINRKRLAESKKLLKYTDYNITEISQMVGFNSVSRFISLFREKFEETP
                     KSFRESK"
     gene            complement(12193..13434)
                     /locus_tag="PK733_04555"
     CDS             complement(12193..13434)
                     /locus_tag="PK733_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOJ09846.1"
                     /translation="MAIRNTYDLRKTIDKVVDEVKITDVHTHLFSTDFGEQLLWGVDE
                     LINYHYLVAETMRLTDIPYEKFWRMSKVEQADLIWDTLFIKNSPYSEACRGVLTVLEK
                     LGLNVADRSLNEYRDYFKKLSVDDYIDIVFKASGVKDVVMTNDPFVDEERKVWLDNYK
                     GDSRFKAALRIDPLLNAWDINYLKLKAWGYNVERELDENTLKEIRRFLSEWIDRMKAL
                     YMAASLPPEFKVPEESSRSKIIEECIIPVSQEKNVPFAMMIGVKKLINPEIKLAGDGV
                     GKGDVNTVEYLCRQYPNNKFLVTMLARENQHELCIAARKFRNLMIFGCWWFLNNPSLI
                     EEITRMRFELLGTSVIPQHSDARVLDQLIYKWIHSRKIIANVLFDKYSDILATGWVIN
                     EDEIIRDVENLFGGNFWRFLD"
     gene            complement(13591..14505)
                     /locus_tag="PK733_04560"
     CDS             complement(13591..14505)
                     /locus_tag="PK733_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ09847.1"
                     /translation="MEIFNKKSNWYKGNLHTHTTLSDGLAEPDESIKAYKDNGYSFVA
                     ITDHRKIYKGKEEDDLLILNGIEFDINDYSSERKAWHIVGIDMDDEVSVEDILNPSDG
                     FITPQYYIDSINQNNGLAILAHPAWSLLSHDDIMGLEGHLGFEVWNTVSDTMTNRGDS
                     TCYVDLIAKKGKYPLIFASDDTHFYNTDLFGGYIMVNSKSLKKDDILENIKKGNFYCS
                     QGPEIKQISIENDEVYVETSPVEFIAFMTDTFYCHDRIARETGKLITEGHYKIKKTDR
                     YVRIECKGEDGRRAWSQVIPLECFLNLR"
     gene            complement(14835..15908)
                     /locus_tag="PK733_04565"
     CDS             complement(14835..15908)
                     /locus_tag="PK733_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09848.1"
                     /translation="MKSPIRVGIIGQGRSGYAIHCAHLKTVPEQFKIAAVVDPIEDRR
                     KRAEKEFGCDTYADYRELLKRDDIDLIVNSTPSYLHVPVTLDILNAGFNVLCEKPLAR
                     RVEDVDTLIATAKKSGKVFAIYQQSRFAPYFRQVKKVIDSGVLGRIAQISIAFNGFAR
                     RWDWQCVQKFNGGNLLNTGPHPLDQALQLFGTDIMPEVFCVMDRCNTFGDAEDYVKLI
                     LKGEGRPLIDLEISSCCAYPSFTYNVQGTRGGLKGTMTHIDWKYFKEEEAPEQHLIME
                     PLNTPEGKPAYCGENLKWYEESWDIPEEEKNLFSTISSKFYNMLYSTLTTGAPLEVTP
                     EQVRQQIAVIEECHRQNPLSREY"
     gene            complement(16170..17753)
                     /locus_tag="PK733_04570"
     CDS             complement(16170..17753)
                     /locus_tag="PK733_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOJ09849.1"
                     /translation="MNWEKFCFKLQDIAPGNNQSHIPNSNDEILTNIRLEAKRNFTEY
                     GIYPDSIHNQNGIWYFLIRDNERKSLVIHGKDNNFNDFEGEEMTVNGKPTKVCSLGNN
                     NCDIIRHIFPFTNPSNHKGRNITIGLGDRLGVASAGHIRLVKDKPVFPVLAQQSIREL
                     NLTGRTYRDVLSAASWDVFQEGYTKGFGADGDHLKTYDEVKMALDNGFTMITLDCSEH
                     INNQVASYSSDEVKQKYSEIELGERNRLESTYLNKSFKLNDVVAISFDQDSFRRIVLI
                     YLKAIKYTLEIYNEVIKNCGRSIDFEMSIDETLTPTTPESHYFVASELIAGGVEITSL
                     APRFCGEFQKGIDYRGYIKQFTEEFELHVKIAQHFGYKVSVHSGSDKFSVFPIIGEKT
                     GGKYHLKTAGTNWLEAVRVISAENPSLYRKIHKFALKHLNEAKKYYHISADPSNIPDI
                     DMLKDHELPSLFDQDDSRQVIHITYGLILQAKDSEGKPLFKNDIYSTLFNYEEEYCKS
                     LEKHIGKHLNLLGIRTDVL"
     gene            complement(18031..19203)
                     /locus_tag="PK733_04575"
     CDS             complement(18031..19203)
                     /locus_tag="PK733_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOJ09850.1"
                     /translation="MDIRQESLKLHEEWKGKIEVVSTVKVENKRDLSLAYTPGVAEPC
                     LAIEKNVDLSYEYTRRWNLVAVITDGTAVLGLGDIGPEAGMPVMEGKCVLFKTFGDVD
                     AFPLCIKSKDVDEIVNTVKLISGSFGGINLEDISAPRCFEIERRLKQECDIPIFHDDQ
                     HGTAVVTLAAMLNALKIVKKDIKDISVVVNGSGAAGIAITKLLMSLGLQDVILCDRKG
                     AIYEGRDGLNAEKAEMAKISNLKMKKGMLPDVIKGADVFIGVSAPGMVTKEMVQSMAK
                     DAIIFAMANPVPEIMPNEAKEAGARVIGTGRSDFPNQINNVLAFPGIFRGALDVRARE
                     INDEMKIAAAKAIASLVSEEELNEEYIMPAPFDPRVGKTVAAAVAEAARKTGSARL"
     gene            complement(19667..20749)
                     /locus_tag="PK733_04580"
     CDS             complement(19667..20749)
                     /locus_tag="PK733_04580"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOJ09851.1"
                     /translation="MGKNEERNELERGTLDRREILKYIGDTSQLFGVKNYRLADGKAN
                     GVRAVDIKNGSGLELTVLPDRALDIAYLSYKGINMSYISKAGIVAPQYYNESGIKFLR
                     SFIGGFMTTCGLIQAGSPCIDEGQEYGLHGRISNTPAEEVYAGTEWTEDTAIMKVKGK
                     VREAAVFGENILLKREISVPYGGNKFYINDTVENYGFKDEPLMIIYHCNLGYPLLTSK
                     SYFLAPSISVIPRDEEASKGQNEYYKFQLPTPGYREQVFYHNLKVDDQGKTFAALINP
                     IQKIGLAIWFNKKQLWNLTQWKQMGEGEYVLGIEPCNCHVGGRAKARQDGTLEFIKPG
                     EIRHFNLEVEIIEGDERISQLEELAK"
     gene            complement(20795..21493)
                     /locus_tag="PK733_04585"
     CDS             complement(20795..21493)
                     /locus_tag="PK733_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09852.1"
                     /translation="MEPNKKPSNLRMAKYYLINALKFYEMKNERITPVLFVIILFTGF
                     ASTLISEKTANDIYLSAMYNIASVFILYLASVVYLYSYMTELRGGECSLKQCLGQVFR
                     KLSKILAAYIAFIAIILTGFFLLIIPGVIFYLMFMFNICYLLDKDIRVTDAFNASKNL
                     TARRKMEIFAIFIVFNLTLLLPIFFIIILASVYGNNLILSFVVTFLSSILTLMQQRLI
                     ALLYFDLEYGTKRA"
     gene            21713..22618
                     /locus_tag="PK733_04590"
     CDS             21713..22618
                     /locus_tag="PK733_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOJ09853.1"
                     /translation="MKHIVSVSLGSSTRDHKVELELLGDKYIVERIGMDGSYDKFLET
                     LKDLDGKVDAIGMGGIGIYLPVKNHTYLIKSALPLMHAVKKTPVADGSYLRNALEPYA
                     ANFVQKKGIINFAGKKVMVTCAVDRYALSEAFVNLGCSITCADLVFTLGIPFVVRSLN
                     GLDRLAKLFAPAVTKLPFDMLYPTGQREENVENPIKFAKYYREADIISGDFNYIKRYM
                     PDDLTGKVIITNTITKENVEELKSRGVSTLVTTTPEFNGRSFGTNVIEAIMITILGKP
                     VDMITVEDYIATSERLGFEPRVVRF"
     gene            complement(22806..24554)
                     /locus_tag="PK733_04595"
     CDS             complement(22806..24554)
                     /locus_tag="PK733_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOJ09854.1"
                     /translation="MEMVNITIDDRNIQVPKNFTVLEAAKQANISIPTLCFLKDINEI
                     GACRMCVVEIKGARSLQAACVYPVSEGLVVKTQSPAIREARKVTLELILSNHEKKCLT
                     CVRSRNCELQKLAEELHISDIRFDGETHKMPLDDFSPSIVRDPNKCILCRRCVSMCKN
                     IQKVAVIDTNERGFDTIVSSAFNMSLNEVPCTMCGQCINVCPVGALREKDDTEKVWQA
                     LANKDLHVIVQTAPAVRVALGEEFGMPIGSRVTGKMVSALKRLGFDKVFDTDTAADLT
                     IMEEGTELINRIKNGGKLPLITSCSPGWIKFCEHNYPDFLDNLSSCKSPHEMFGALLK
                     SYYAEKMGIDPSKIFVVSIMPCTAKKFEAQRPELSSTGYADVDVVLTTRELGRMIRET
                     GMDFKDLPDSDFDDPMGNATGAGVIFGATGGVMEAALRTVADVLTGKSHEDIEYLDVR
                     GVEGIKEAQVDVGGMKVKAAVAHGLANARKLLDRIKAGEADYHFIEIMACPGGCVNGG
                     GQPIQPSEVRSWIDIRKERAKAIYEEDRDLPIRKSHENEKVQKLYKDFFGEPGSHKAH
                     ELLHTHYAPKENYPEK"
     gene            complement(24573..>25368)
                     /locus_tag="PK733_04600"
     CDS             complement(24573..>25368)
                     /locus_tag="PK733_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOJ09855.1"
                     /translation="KGPEWFSSIGTEKSKGTKVFAVGGKINNTGLVEIPMGTTLREVV
                     YDIGGGIPNGKKFKAAQTGGPSGGCIPASHIDTPIDYDTLIQLGSMMGSGGLIVMDED
                     NCMVDIAKFFLQFTVDESCGKCPPCRIGTKRMLEILERITGGKGEEGDIEKLEILAKN
                     IKASALCGLGQTAPNPVLSTLRYFRDEYEAHVKDKKCPAGVCKSMMHYVIQADRCKSC
                     GICARNCPVGCISGQKGVPYVINEDKCIKCGACMQKCPFKAIFKNA"
BASE COUNT         7489 a         5212 c         3915 g         8752 t
ORIGIN      
        1 catattatgc tttttgtttc tgggtacata agctatcatt ccaggaccta cttcctgctt
       61 ttcgtctcct atcataagta tagctctgcc ttttaaaatg acatatattt cttcttcatc
      121 gtcatgggta tctacctgag tactattccc tttctctaca gacacatacc caagagcaat
      181 agtttcagct tcttcacggt taacaagcat ctttcttttt acatcttcat tttcatccca
      241 tattttccta acaagcataa tattaccccc ttgtagattt tatagcaaat gcaccaatat
      301 gtctcccgcc cctatgtgcg ggagacgaat tagtgcaatt ttgctgcatt ataatatgtt
      361 aataatgcgc ttatgattac aattcacttc cactatcttt gtctacataa agcactgcat
      421 tatcatgggt cctcaatatt gtagcaggac atgcggtact gatttcgtca ttaactgttc
      481 tggtaactgc ttcagtttta gtcttgccag gtaccataca gaaaacatac ttgcatgcca
      541 taagagtagg tattgttaaa gttaatgcgt atttaggaac atcatcaatg gttttgaaac
      601 aaccatcgtt tacctgctgc tgcctgcact tttgttcaag ctcaactacc tttacaactt
      661 ttttatcatt gaagtctgct actggcgggt cattgaaagc tatatgacca ttttcaccaa
      721 ttcccataca tactaaatca gctggatact gttttaataa attgctatat ctttcacatt
      781 ctttttctgc atcttttgca cttacatcaa tataatttac agacttgaaa gataccttgc
      841 tgaatattct atcttttaga aaattgctaa aagcctgtgg ggcatcttta gatatcccaa
      901 catactcatc catatggaaa gcgttaattt tgctccagtc aattccctta tcttctataa
      961 gtgaagccag aaattcattt tgtgatggtg ctgctgcaaa aataacgtta acttctccct
     1021 tttgagaaat aagttcttta attttagatg ctgcttcttt tgcagctcct tcacccattg
     1081 cttgtcttgt ttcataaatt ttaactctta atttgtctac ttgcatttct ttaatgctgc
     1141 tcatttataa gcctcccttt aacatcattt gtgatatata taaatttttg ttgcaagctt
     1201 aattgtattg aaatcccaca ttttatcact tgttttatca gctaaagcaa atcttgaatt
     1261 tcacatcttt aatatgagat atcttatatt ttagctactg aacttcttat tacaagtgaa
     1321 ggctccagta ttattgatgt atcttcatat ccatttccct caaggctatg cagcagtatc
     1381 cgacatgcca gctttcctgt ttcaaatgca ggttgattta cagttgtgag tggaggactc
     1441 accatagatg aaacctcaat attgtcaaaa cctacaacag aaacctcctc aggcactttc
     1501 actccattac ccagcagttc ctgaataatc ccaaaggccg tcatatcatt tactgcaaaa
     1561 atagctgtgg atccatttct tatctgcata aacctttttg caaggtacct tccattttca
     1621 aattcatagg tagcattctc atattcttct tctttttctg aaacaattac attattttca
     1681 ttaaaaggaa taccattttt tgaaagtcca agcttgtacc cttcaaaagc atctcttctg
     1741 ctcttccggt tcagcggaga agtaacaaag gttatgttgt tgtgtccctg ttcaataagg
     1801 tattctgttg caatcaatcc tcccttaata taatcaaaac ctaccttgct gcatttcatg
     1861 ccttctatat cctgatcaat ggttacaatt tttatccctt tttcctgcat tatttcaaga
     1921 aatttatagt tttcacctat agatgaaata attattccct taacctgctt ttgatataat
     1981 gtttcaatgt actttctttc tgtagctgca tctctgaaag aattgcacaa taaattatta
     2041 aaacctttaa gccttgcttc ctgctctact ccaagaacca tctcaggata aaacgggttg
     2101 gaaattgtag gaataataac tcctatatcc atgctatcat tctttttcag cattcgtcca
     2161 attatattag gttcataatt cagtgtttct acagcatcaa gaatcttctt gcgtgtctgc
     2221 tcctttacag ggtagccgct tttgctcaat actctggaaa ctgttgctgt agaaacacct
     2281 gcttcttttg caatatcata tattgttgtt tttttattat gcaatacact tctcctcaat
     2341 aaacatttat tgttttattt attaaaacca gtatgcttat gtagtaatcg tttactgttt
     2401 atcttataac actttaaaga aaaaatccag ctattttttt gtggtatgct atattttctt
     2461 cctatgaaac aaaatatggt aatatacgat tttccaagta ttaaatttga acaaagcaac
     2521 gggttgtaga cataaactct acaacccgtt gctgaaatga atactatacc tcaaaatcga
     2581 ctcttgccat atttttcaaa tatagaaaat gcgtcctttc taataccagg tattaaccct
     2641 gtatttttat cggcagccct gtatccatag aacggttgca agccaaggta aactccagag
     2701 ttcgaacggc atcttcataa ggagaccgga tttttgagct gtcacctgtg agtactgcat
     2761 caatgaatgt acgatcacaa agaataccag cactgccttc ctgtttgtat gtaatttcac
     2821 cggctttaga aacgctcaat gcaccatcac ctttaattac gaaacctttg gatttatcgg
     2881 atttgctgtc tgcatctgca ccgttattgc cgatatcgtt attatcctta taaccgaata
     2941 ttttcaaatc actcaaaatg cgtaattctg ctcttgcatc acgtgaacta aagacaattt
     3001 tactgtcagc agcattacca cctgtagcat agcatccggt cgaaattgca gcaagcgcac
     3061 cacttttgaa tcgcacaaca gtggttgaca aatcttcggt gtcatagtct tcaaaatcgg
     3121 taacaaagcc ttttgtcccc atagtaaata ccatatccgg ctcatcaaat acatatctta
     3181 taatgtcaaa ttgatgaata gtctgctcaa caatctgccc gcctgaagtt gaccgctttc
     3241 tccaccacgg aacaccagga acgccgccaa tacgtataca atcaacaaat ataatttcat
     3301 tttcttttat aaatttaaca tttggtccaa caagattact gtagcggcat tggaaacctg
     3361 cagccgtaat aagtccggct tttttaactt catcacgaat ctgtctggcg agatccatgc
     3421 ataatgcaac cggcttctca acaaaaaaag gaatgcggcg gtttatagtt tcaaattcga
     3481 tctctccatg ttctgtaggc ggtatgcaga taaataccat atcaggatta attgcgtcat
     3541 acatttcctt ataatttgta aatgcttttc caccgcctgc tttagaaaca aagctttcag
     3601 cccgttcagg aatcaagtcg cagaaacctg ctaattcaat atcttgaaag ttcaaaaaat
     3661 ggctaagatg ataaccacct attcctccgc aacctatcat tgcaagcttt ttcataaaca
     3721 attacctctc atttccgatt attttttatt agtactcata taacctgtta ttgcatataa
     3781 ttttgtatca ttaactatga ttactaatta tctgctactg ctaatattaa ttattatgtc
     3841 aagtgcgtct gatgtaattt tgtataaata ttcgggacag tcctttatga taccaagttc
     3901 agctgtaagg taaccattat aacctgtttt acgtagcgca gggataactt tgctccaatt
     3961 agcatcacct tccagaagat ttacaaaaca tcctcctctg ttaataccat tactgcgttt
     4021 aaaatctttt acatgaattt tccgtataca ggtactgaga atttcaatcc aatattcggg
     4081 attggaaaat gctattacat ttcctacatc aaaatatgct ccgatatatg gacaatcaag
     4141 tctgtcaata aagtttttca tatcaaatgg tgaaataaaa aagccattcc acacattctc
     4201 gagaccaaca ttaatttttg cggattctat ttctgatttg agttcttcga ttgccataaa
     4261 tgaattttcg tatgcttgca ataaagagat actatctgtc ataccgcccg gtactgtgag
     4321 aacaccgtct gcaccaagct ctgccgcaca ttccatttgt ttacggatta tatccttgcc
     4381 cgattcgcga acatctttgc tatccgagcc taatttgcct ccgctcaatg atgttgaaat
     4441 actggcaatc ggaagattat attcctctgc aaggtggttt atttcagtga atatctcctt
     4501 tccggagctc attgtcaatg agtgactgga atagttatct gcgtccaaat tcagctctat
     4561 tccatcaaac cccgcattgg aaatatcgcg gaacatttcc tcaaaggtaa tatgacttgg
     4621 taccgaccag gcatttattg cttttttcat aacattttct cccttccttt attaattata
     4681 atttttattg gtagtttttg ttttttagca aaagctgttg aaatattgtt tcatttaaat
     4741 gtatactatg tttaatgtgt gctatgttga atgtatgcta tgttaaatgt atactatgct
     4801 tacagaagca acaatataca attcggttat atatatggac aaatcaactg tatattataa
     4861 tatgttaaat attctcagtt gattggacat ctaatttaag ctattactat gcatataaaa
     4921 tattgcagga ggaaataaat aataattaag atgctatgga aaatcagtca tattatcgta
     4981 tttgggaaaa ttcagggtgc aatgaggaaa ttaatgacca ttatcttgta gtaaactgcg
     5041 caggatgctg tgttttgcct catccttttg ctactcacaa taaatcagga cggaaagatt
     5101 attatttgtt atatatatgc aggggaatga tgaaagttct ggtaaacggt gagatgatgg
     5161 acatgtgtgc gggtcagttt attatatttc ccccggagtg tgaatataaa tatacaaggc
     5221 ttggagacga ggaagtaata tactattggg ctcactttac cggttatggt gcccgcggac
     5281 tgctggaaaa ctgcaagcta tatgggaaaa acatatttaa tgccggcttc aacgaacagg
     5341 taatacgtga attcaacaat atgttttcag aatttatccg cagggattac tgttttgaag
     5401 cagctgcagc atcacatctc atctcggcct gcataaactt aagtagaagc atgaccaatt
     5461 tagcaaccag cagcgacgca gttaatccag atagaatata tgcatcaatt aagtttatac
     5521 acaaaaattt caattccaat atatcattaa aacagcttgc cggcattgag caccttagta
     5581 tcagccgtta ccggacactt ttcaagcagt ataccggctt ttcaccaaaa aattatatta
     5641 ttgcacttcg tataaataga gcatgtgagt taactgtaca aaccgattta tctttaaaag
     5701 aaattgctga atcggttggc taccacgatc agctttattt cagccgcatt ttcaaagctc
     5761 gtacaggtat tgcaccaaat tgctataaga aaaattatca gaataatgca taatattaca
     5821 agcgtcaaat cataaaatta agcaagtaat taagcaagaa aaggtggaca ttaagttcac
     5881 ctttccctct tgttaatctt aaaattttca aagtcagagt ttatatcgaa gtttataacc
     5941 tcatataaaa ccactatccc tttaaaggta agttatggtt aattaattac attccggttc
     6001 caatggccct gccagttctt ttaagaaaaa taatttacct ggcaatgtgg gcatatagct
     6061 tgcgggtatc caaatgttag gtccgtatct tagtgctacc caaagtgata ctccctgcca
     6121 catcatccct cttcccaagg ttccatcaca accaccaatg attttatcag actgcaggaa
     6181 taaacctggt ccaatagtgt ttgcacggca tggaactaat gtagtcctgc tcttaaagct
     6241 ggttattgca ttttgctcga ttgtaagagg atgaagtttt gcagctattc tatgtgtttg
     6301 cttcttccat tcttcttcac agcaaaaatc tgctgcaaaa tgaggctccc agaatgctat
     6361 atggaacatt cttcctattc caaatactgt tataagttta gccccttcag cctttaattt
     6421 tgctatcttt tcgccatatg taggcaaatt atctttcgtt gcgaagttcc tctgtgatac
     6481 aggtactgtc aaattgccaa gtggtccata aaaagcacct tccatggcat tttggaaagc
     6541 tccgggattt gttgactcaa gagtattgcc ttctgagtcg ctccactcat ccatattgaa
     6601 accatataca tgggaacaat caacattcca ctctttcagg aaataaacag cccagcggta
     6661 cattcccata gggcctacag gaagaatcag aatcagcttt cttccttcat cttttgcctt
     6721 ctttatttct agagcaattt catgtcccat taatacatta aaatcataaa tgttatcaca
     6781 tggtacagga gcgaaatcct cgttccagaa agtctgtctt ttagttattt cttcaggtgg
     6841 gttggaacag cattcatcga tttttttcat atcccagcct gcagggaaaa atccctctaa
     6901 cagtgaaccc ttaactgtgt ccaaaaagtt cataatacaa cctcccttaa acacttgttt
     6961 tagttgtcat gtatattata atcctaaaaa gatataattt caactattct ggcttattcg
     7021 ggtgcattta tcaaatctga agaaatattt accaggggct atggctctat aaccatccgg
     7081 taagttttgc aagcaacctt gtatactcta taaagtcagg ccatgaaata tctggcggca
     7141 caccatggtc gcaccccgga ataaaccctc catccttcag aagaggtgga actaccctca
     7201 ttacctcgtt aaacatatcc ttcccacctt tagctatagc ccttttatca atgccccctg
     7261 tataagccat acttttgcca aatactcgcc tatactcaac tatatcgtta tgggcagcaa
     7321 cttctatagg atcacaagca tttattcctg attcaatcca tataggtatc aattctccaa
     7381 tataaccatc agaatccata tcaataatgg agcagccact ggctttaatc tcttttaccc
     7441 agcggatata tgaaggcatt agaaactccc tggtcatagc aggagaaatc atactatgag
     7501 ccttatatgc catgtcttcg gatatatgta ccttatccag ctccatttta ttgataaacc
     7561 gggctagaag tcttgaaata aattctgtcc agaattcaat catttcatga ataaactccg
     7621 gctcctcaat aaatttgata cacagatttt cgaaaccaag ccattcacgc aactgccaga
     7681 atggtccgtt aaaagaaatg cctgcaacat aatccctatc ctgcaacttg ccacatattt
     7741 catcaaggtt ttccgtcagc cgttcaggag aatcggcatt ataacgtttt ttcatttcct
     7801 cccaatcttc tctgctttgg acaggaaact tgtgccactt tcttgtgaca aaatcctttg
     7861 cagcccttat ataggtataa tcaaattcgt ctgatatttc tgttatagca cccatccagt
     7921 cctgaacaat atagtgtcca tctttatgct ccagtacctt ttcctcaaac tgtggaatca
     7981 ttttgaaatc aactgttaaa tatgtttttt taggagcagg aggattagga attccgagta
     8041 tatccattag tgcatcgaag taattaactc cctccggtaa tccctgcttg tgccaggctg
     8101 caagagttga ttctcttggt actcccggtt gaagaggtat tttgtcagga ttcccaaata
     8161 gcagtgtttc aatgtatctt ttcctttctg tcattttctc atttgaccac tttttcatag
     8221 tgttcacact cctgttaata tttataatat attaaattct aatgacttta atttattact
     8281 aagtcttaat ttgaaaagtt tttgatactt ttcgcaagtt atgttaatat agtaaaataa
     8341 aatgctacta tcattaaaca tacctaccta cctaaaaata ttaccgaata tttcacaaaa
     8401 agtcttaatt ttatttgctg cattattaaa tattaacgta tcttgctttt tttatttact
     8461 tcttaatttt tgttttatat aattatagtg aaatgcgatt atatattgaa atatggttct
     8521 atgattttaa tgtaatgtag tatcttttat gcgttatcta tttaaggaaa ttatgataac
     8581 ttagtataat ttgggtggtg accgtaaatg cctgatttac aagataaagt ggattttaac
     8641 aatctctatg tatcaaaaac tgctgatttt attactgatt atcgtgatag taaggtagat
     8701 caggttttat ccctcgagca ttaccacgat tcatatgaac tggatttttt cataaaagct
     8761 gatatacaaa tatttgtaaa agatagaaga tatgagatta gcaatggtga tgtgcttttc
     8821 atcagcgaat atgatgtgca caaagtctta tataatattg gtacacatta cactagatat
     8881 gttattaatt tcaaaaaaga atttctcatt gatcttttaa gattctttaa gattgataac
     8941 cttttggaaa aaatacaaaa aaatgattac aaaaaagtca gagttagtat taaccgaagg
     9001 aatgaatttg aaagccaatt taaaacatta cttgctctat atgattcgtc acgggaaaat
     9061 tgtaatgctg aaactgctgt attaattaag tcatatttag ttatactcct tacccgcttt
     9121 aatgagacac ttaagaatgc aaagcccatt tgtgaacagc aaaaaaaaga tcattttgtc
     9181 agcagaataa tagattttat tgactccaat tatgcgggtg taataaatct tgatttgctg
     9241 gaaaaggaat ttcatcttaa taagtattat ataagccatg ttttcaagga aataacaggc
     9301 tttaccgtta tggaatacat tcaaaataga agaattattg aagcacagat actgttaaaa
     9361 agcaccagta aagatatcac ggaaatatgc tatgattgcg gctttaataa tgtacaacat
     9421 ttttatagag tattttcaaa aataacaaat attactccat acaattacag gaaaagaggg
     9481 taagataata agagatctgg agaacaaggg gacagttttc ctgttttaat attaggtaac
     9541 tataactatc ccctgctttt ctattacttg cgttttactt gcttatttcc cttgctcatt
     9601 tacatactat ctcattaccc atctttctac ttcctaatta aatctttcaa cctgaacatt
     9661 ttcttttcct ttatactctt gtttgcgcat atccctatca ttatggattt tactccatcc
     9721 caggatccgg cgaaatgatt tagggggtca gacacaccac cgcggaatat catattgcgt
     9781 aatctttcat ctccgccccc atgtccgcct gacgcctttg gcacatcata tgttacaact
     9841 tcgccttttc tgttaaataa ctgtatctgg tagtttggtt cctttacctt atgtccgctg
     9901 tggtattctg ccgcttcgag tcttccatta gtaccattta tgcttatctt ccaaccttca
     9961 taagggctaa atgctattaa tgaatatgta agtaaagtac ccttattgta ttttactgca
    10021 actgacattg agtcatatat atttatttca tcagcaaata cacatctgtc acggaaatat
    10081 ccgtcttctt tttctgcttt aaagtaaaaa tcctgcataa aaggattttc atgatatttc
    10141 acagcgaact cgcatgttga ggcatattgg caggtactgc atctctctcc tttattttcc
    10201 cttgtaggac catagaatct taggtttcca tttgcatata tttcttcagg ctcttcttct
    10261 atccaccagt taacaaggtc aaaatgatgc gtggatttat gtacaagaag cccaccgcag
    10321 ttttccagtt ttctatgcca tcttctaaaa taatctgctc catggctagt atcaagaagg
    10381 tattcaaaat ctacgctaag tacatcacca actgcacctt ctttaattag ctctttaact
    10441 ctggtaacat aaggtgcaaa acggtagtta aatgtaacta taactttttt tccagtcctt
    10501 ttttccgcat caagtattgc attacactta tcttcatcta ttgtcatcgg cttttctgtt
    10561 attgcatcac atccatattc aagggtctta ataatgtatt cgtggtgaaa tctgtcaaca
    10621 gttgtaatta ttgcagcatc aggttttgtt tctttcatca ttacatcata atctgtaaaa
    10681 acaggtatgg atgaatctgt atgatccctg atatattccg ctcttgtcct gttaatatca
    10741 aatattccca cgatttgtgc aacatcttta tacccctcta taaggggctt ggcaaacatt
    10801 ccaaaagctc ttccacttgc gcctactaga acatattttt tcattcttta gcatctcctt
    10861 ttcgtattat cctttttctt ttcatatttt tcttaacttc atattgcttt acttcatgtt
    10921 gcttcacttc atgttgcttc acttcatgtt gcttcacttc atgttgcttc ggggctgtaa
    10981 cagagcaaaa tttatatact ctgcaataac atactaaata atgtccgcac ttagatgcag
    11041 ggattacgcc ttaacataat accgatgtaa ttcgtaagta atccttaata ttaaaacata
    11101 atacatattt gattcttctg caaaacgctg ttacactgca aaagtctaat ttggaacatg
    11161 gctttctgca gtggaatcat atttacaatt ataacattaa tgtttttcct ttactttata
    11221 aatcttgttg tatataattg aaaatgtata tatatattgc ttttttaggg ggagatgtaa
    11281 ttgcaggtta tatactcacc gaacacattg gacacagatt tttatataga gcatacaaaa
    11341 attgcttcag gcacaagcat gaaaacagag cactatcatg atttttatga gctttacttt
    11401 tatcttggcg atggaatgcg ctattttata ggaaataaaa ctttctatgt gaagaagtat
    11461 gatttaattt taattgacaa atttacctac catagaactt catataaaga aaagggtttt
    11521 aaagaacgat tgttgatata tctttatgat gatgtaatta atataatagg caatgaaatt
    11581 ttaaagcaaa aaatagtcaa cctctttaaa aagaaaaaga tttctttcta cagcagtttt
    11641 agcaaatata tgcttgatac ccttatgaac agagttctcc ctaaatacta taataaatcc
    11701 aatccttcta taagtcagct tcaagccagg ctggtcatgc tggatttgct gcttgaaata
    11761 gctgaaatgg atgaaaatgg tcagctgatt gaggataatt caattacaac gccccaggaa
    11821 aatcgtgtct cagatatagt aagttatatt aactccaact atagtaagca aattactctt
    11881 gatgaattat caaaaatatt ttttattaat aagtattatc tttgccacat ttttaaagaa
    11941 gtgacaggta tgagtattac agattttatt aataggaaaa ggctcgctga gtctaagaag
    12001 cttttaaaat atactgatta taatattaca gaaatttcgc aaatggttgg ctttaacagt
    12061 gtcagccgtt ttataagtct tttcagggaa aagtttgaag aaacgcctaa aagttttcga
    12121 gaaagcaaat agggcactgg ggacacatct ccaaaataat tcaaagagtg ccccctagtg
    12181 gtaccaagcg catcagtcta aaaatctcca gaaattccca ccaaacagat tttctacatc
    12241 ccgtataatt tcatcttcat taattaccca acctgttgca agaatatcac tgtacttatc
    12301 aaacagtaca tttgcaatta tttttcttga atggatccat ttatatatca attggtccaa
    12361 tactctggca tcggaatgct gtgggataac acttgtccca agcagttcaa aacgcattct
    12421 ggttatttcc tctatcagac ttggattatt aaggaaccac cagcatccaa aaatcattag
    12481 atttctgaat tttcgtgctg caatacagag ttcatgctgg ttttcccttg ccagcatagt
    12541 aaccaggaat ttgttgtttg gatactgtct gcaaaggtat tcaacagtat ttacatcacc
    12601 ttttccaaca ccatctccgg ccagttttat ttcagggttt atcagttttt taacaccaat
    12661 catcattgca aagggtacat tcttctcctg cgaaacaggt ataatacatt cctcaataat
    12721 ttttgatctt gatgattctt caggaacttt aaactccgga ggcagtgatg ctgccatgta
    12781 taaagccttc atcctgtcta tccattcact taggaagcgt ctgatctctt tcagagtatt
    12841 ttcatcaagc tccctttcaa cattataacc ccatgccttg agctttaagt aattaatatc
    12901 ccatgcattt aaaagggggt caattcttaa agctgcctta aatcggctgt cacctttata
    12961 attatcaagc cagacttttc tttcttcatc aacaaaagga tcattagtca ttactacatc
    13021 cttgacgcct gaagccttaa aaactatgtc tatataatca tctacagata actttttaaa
    13081 gtaatctctg tattcattta agcttctgtc tgctacgtta aggcccaact tttccaatac
    13141 agtaagtact cctctgcagg cttcgctata gggtgaattt ttaataaaaa gagtatccca
    13201 aattaaatcg gcctgctcaa cttttgacat cctccagaat ttctcatagg gaatatctgt
    13261 tagtcgcatt gtttcagcta ccaggtagtg gtaattaata agctcatcca ctccccatag
    13321 aagctgctct ccaaaatctg ttgaaaacag atgtgtgtgt acatcggtta tttttacttc
    13381 atcaaccact ttatcaatag ttttcctcaa atcatatgta tttcttatgg ccatacaaaa
    13441 atcagtcctc cttttgtatt ttatataatc aattaaataa tttattgtta cccaaacttc
    13501 aattaataat atagaaatat cttcagcaat ataattaata ataacttata ttcccttata
    13561 gtagagcata taattcactt gtcagattat tcaccttaag tttaaaaaac attctaaggg
    13621 tataacctgg ctccaggctc tccttccatc ctctcctttg cactcaatac gtacatatct
    13681 gtctgttttc tttattttgt aatgaccttc cgtaattaat tttccggttt cacgggctat
    13741 cctatcatgg cagtaaaaag tatctgtcat aaaagcaata aattctaccg gagatgtttc
    13801 tacataaact tcatcatttt ctatactaat ttgctttatt tcaggaccct gagaacaata
    13861 aaaatttccc tttttaatgt tctcaagtat atcatccttt tttaagcttt tactattaac
    13921 cattatatac ccaccaaata aatctgtgtt ataaaaatgt gtatcatcag aagcaaaaat
    13981 taaagggtat tttccttttt tggctattaa atcaacgtag catgtggaat cccctctgtt
    14041 ggtcatagta tcagataccg tattccaaac ctcaaaaccc aggtgacctt ccaatcccat
    14101 tatgtcgtca tgggaaagca gcgaccatgc aggatgtgcc aatattgcaa ggccattatt
    14161 ttggtttatg ctgtctatgt agtattgagg agtgataaag ccatcggaag gattcaatat
    14221 atcctcaaca gagacctcat catccatgtc aataccaaca atgtgccagg cctttctttc
    14281 tgaagagtaa tcgtttatgt caaattcgat gccgttaagg atgagaagat catcctcttc
    14341 tttccctttg taaatctttc tgtgatctgt tatagctaca aaactgtaac cattgtcttt
    14401 ataggccttt atactttcat caggttctgc cagaccatct gacaatgttg tgtgggtatg
    14461 aagattgcct ttataccagt tgcttttttt attgaatatt tccaaaaaga attctcctct
    14521 catctgttat tccataatca tattattcga taatcagtta ttaataatat tccctttaat
    14581 aatatccata taattatcat aatacgaaat aatgtaaaag ttataaatat aatatcatat
    14641 taatgataaa agtaaagttg ggttacattg ataaaaaaag attgccgggt ttattccgac
    14701 aatctttttt aaaagtaata gactttattc ccgtattaaa ctgttttcat gagcagatgt
    14761 gtatctgaaa taatacacct gaaatattat aattgaatta atattctatt cttaatactc
    14821 tcttgaatct attcttaata ctcccttgat agcgggttct gcctgtgaca ttcttctatt
    14881 acagctattt gctgtcttac ctgctcaggt gttacctcca gcggtgcccc tgtagtaaga
    14941 gtgctatata gcatgttata aaacttgctg gatatagtgc tgaaaagatt cttttcttct
    15001 tcaggtatat cccagctctc ttcataccat ttaaggttct caccacagta tgcaggtttg
    15061 ccttctggag tatttagcgg ttccataatc aaatgctgtt caggagcttc ctcttcttta
    15121 aagtatttcc agtctatgtg ggtcattgtt ccctttaagc cgccccttgt accttgaaca
    15181 ttatatgtga atgatggata tgcacaacag gatgatatct ccaaatcaat aagaggacgt
    15241 ccttcccctt taagtataag tttcacataa tcctctgcat caccaaatgt attgcagcgg
    15301 tccattacgc agaacacttc cggcataata tcagtgccaa acaactgcaa tgcctggtca
    15361 agaggatgcg gcccggtatt taggaggttg ccgccattaa acttctgaac acactgccaa
    15421 tcccatcttc tggcgaatcc attgaaagct atactgattt gagctatcct gccaagtact
    15481 cctgagtcta taaccttttt tacttgtctg aaataaggtg caaaacgtga ctgctgatag
    15541 atagcaaaaa ctttccctga tttttttgca gttgctatta atgtatcaac atcttcaact
    15601 ctacgggcaa gaggtttttc acataatacg ttaaatcctg catttagaat atccagagtt
    15661 acaggaacat gtagataact tggagtagaa tttactataa gatcaatatc atccctcttt
    15721 aacaattctc tgtaatctgc ataagtgtca catccgaatt ctttttccgc tcttttcctt
    15781 ctatcttcaa taggatcaac tactgcagca attttaaact gttcaggaac tgttttcagg
    15841 tgggcacaat gaatagcata accactgcga ccctgcccta taattccaac tctaataggt
    15901 gatttcatat acattcgcct ctctttttta aaattaagca actacttcca ttgcttccac
    15961 aaacattagg gtgcatatta ttctgtaaat ccttattaac ttttaaaata tgcgccccaa
    16021 aatcgctttc tattgatttt catgcttctg actaatattt tatatcgtat aataaatcta
    16081 ttcattaact ttcttataaa atagttgtac ttttttaaaa caaatagaat gtataaattg
    16141 ccttaatgta cacttccggt aagtgaattt cataaaacgt ctgtcctgat accaagcaaa
    16201 tttaaatgct tcccgatgtg cttttcaaga gatttacaat actcttcttc ataattaaac
    16261 agagtagaat atatatcatt tttaaataat ggtttgcctt cggaatcttt tgcttgtaat
    16321 atcaacccat aggtaatatg tatcacctgt cttgaatcat cctgatcaaa gagagaggga
    16381 agctcatgat cttttaacat atctatatcc ggtatatttg aagggtcggc acttatatga
    16441 taatattttt tcgcttcatt aagatgtttc agagcaaatt tatgaatttt tctatataat
    16501 gaaggatttt cagcagatat aacccttaca gcttcaagcc aatttgttcc tgcagttttc
    16561 aggtgatatt tccctccggt tttttctccg ataatgggga atactgaaaa tttatcactc
    16621 ccagaatgta cacttacctt ataaccaaaa tgctgtgcta ttttcacatg gagctcaaat
    16681 tcctctgtaa attgctttat atatcctctg tagtcaatac ccttctggaa ttccccacag
    16741 aatcttggtg caaggctggt aatttcaaca ccacctgcaa ttaactcaga agcaacaaaa
    16801 taatgtgact ctggtgtggt aggtgtaaga gtttcatcaa tagacatttc aaagtctata
    16861 cttctgccgc aatttttaat aacttcatta taaatttcca gggtatattt aattgccttt
    16921 aaatatatta acactattct tctgaaactg tcctgatcaa atgatatagc aacaacatca
    16981 tttagtttga atgatttatt taaatatgta gattccagac ggtttctttc acctaattct
    17041 atttccgaat atttttgctt aacttcatct gaagaatatg atgcaacctg attattaata
    17101 tgttctgagc aatctagtgt tatcatggta aaaccattat caagcgccat ttttacttcg
    17161 tcatatgtct tcaagtggtc tccgtctgcc ccaaagcctt tggtatatcc ttcctgaaaa
    17221 acatcccagg aagcagcgga aaggacatct ctatatgtac gccctgtaag gtttaattcg
    17281 cgtatggatt gctgtgctaa aacaggaaaa acaggcttgt cctttaccag ccttatgtgc
    17341 ccagctgaag caactccgag tctatctccc agtcctatag taatattcct tcctttatgg
    17401 tttgaagggt ttgtaaaagg aaatatatgt cttattatgt cacaattatt gttacctaga
    17461 ctgcacactt ttgttggttt accgttaact gtcatttctt cgccttcgaa gtcattaaaa
    17521 ttattatctt tgccatggat tactaaactc tttctctcat tgtctctgat aagaaaatac
    17581 catatcccgt tttggttatg tatagaatca ggatatatcc catattccgt aaaatttctt
    17641 ttggcttcaa gccttatgtt agtcaatatt tcatcattac tattggggat atggctttga
    17701 ttatttcccg gggcaatatc ctgcagctta aaacaaaatt tctcccaatt catgctaaaa
    17761 cctcctcaat aaagttaaaa atttatattt acactataat tgatattata tgctatatta
    17821 tgctatgaaa tcaaggataa tagtaatcga ttactaagca aatccattca ggagaaagta
    17881 aaactggtga gtcccgggat aattatccct gatgactcac cagcgaatat taattactga
    17941 ttaactgaaa ttatttatta ccttgtaatt ttgcaacact agtccttcta tttgcattac
    18001 tgttatgctg cactaatcat tccgattacc ttacagtctt gctgaaccgg ttttccttgc
    18061 tgcttcagca actgctgctg ctactgtttt tccaaccctt ggatcgaaag gagccggcat
    18121 aatatattct tcgtttaatt cttcttcact tacaagcgat gcaatagctt ttgctgctgc
    18181 aatcttcatt tcatcattta tctctcttgc tctaacatca agagctcctc tgaaaatgcc
    18241 cggaaatgca agcacattgt taatctgatt cgggaaatcg gaccttccag tacctataac
    18301 tctggcacct gcctctttag cttcgttagg cattatctca ggtacagggt ttgccatagc
    18361 aaaaatgata gcatcctttg ccatgctttg aaccatttct tttgttacca ttccaggagc
    18421 agatacacca ataaatacat cggcaccttt tataacatca gggagcatac ctttcttcat
    18481 tttcaggttt gagatcttag ccatttcagc tttttcggca ttcaggccat cccttccttc
    18541 atatattgca ccctttctgt cacaaagaat tacatcctgg aggcccaggc tcattaatag
    18601 tttcgtaatt gcaatacctg cagcacctga cccattaacc acaacagaaa tatccttaat
    18661 atcctttttt actattttga gtgcattcaa catagccgca agagttacta cagctgtccc
    18721 atgctgatca tcatggaaaa taggaatatc acattcctgt ttaagtcttc tctcaatttc
    18781 aaaacatctg ggtgcagaaa tatcttccag attaattcca ccaaagcttc ctgaaataag
    18841 tttaactgta tttacaattt cgtctacatc tttagattta atgcataacg ggaatgcatc
    18901 tacatcccca aaagttttaa acagtacaca ttttccttcc ataaccggca taccggcttc
    18961 gggaccgatg tcccccaatc ccagaactgc agttccatca gttataactg caacaagatt
    19021 ccaccttctt gtgtactcat atgaaaggtc gacatttttc tctattgcca agcatggctc
    19081 tgcaacacca ggtgtgtatg caagagaaag atctctctta ttttcaactt ttactgtact
    19141 tactacttca attttacctt tccattcttc atgtagcttt aacgattctt gacgaatatc
    19201 catttttttc tcctctccag tttatacgaa ttttttaagc agttttcctt acataggcaa
    19261 ttatcttttt atcaacttat ttttattgtt tttcgtgtat actaaaaaat taacatacta
    19321 tatcatcctt atatgttatt cataatatag gctgatttta tgtgtcacaa atatatttta
    19381 ctttacagtt gcatcaatat caatatatca actaatatat tgttattttg gttgatattc
    19441 tagtaatata cgtcaagttt aagtacagta taacacgact acgggtgcat attttaaaag
    19501 tgaagaaata tttacaagga acatcaatta tcagagagaa gagtttctcc tgcctagcta
    19561 cggtagcttc atttatgctc cttactaata ttttcttcac ttaaattata tgcaccttat
    19621 tgtttgctat ttaaatctat atgattaatc gcttataatt ctacttttat tttgccagtt
    19681 cttccaactg actaatcctt tcatcaccct ctataatctc aacttcaagg ttaaaatgtc
    19741 ttatttctcc aggctttatg aactccaaag taccatcctg ccttgctttt gccctgcccc
    19801 ctacatgaca gttgcatggc tcaatgccaa gtacatattc accttcaccc atctgcttcc
    19861 attgtgtcaa gttccacagc tgtttcttgt taaaccatat tgccaatcca atcttttgta
    19921 ttgggttgat taaagcagca aaggtctttc cctggtcgtc gaccttaaga ttatggtaga
    19981 aaacctgctc tctgtaacct ggtgttggta gttggaattt gtagtattca ttttgccctt
    20041 tggatgcttc ctcatccctt ggaattactg atattgaagg agctagaaaa tacgatttac
    20101 ttgttagtaa aggatatccc aggttgcagt gatatataat cattaaaggt tcatccttaa
    20161 aaccatagtt ttcaactgta tcgttaatat aaaatttatt cccaccatat ggtacggata
    20221 tttccctttt tagaagaata ttttctccaa acacagctgc ttctcttacc ttgcccttaa
    20281 ccttcatgat tgcagtgtct tcagtccact cggtacccgc atacacttcc tcggcaggtg
    20341 tattggatat ccttccatgc agaccatatt cctgaccttc atcaatacag ggtgagccag
    20401 cttgaataag tccacatgta gtcataaatc cgccgataaa actcctcaga aattttatac
    20461 ctgattcgtt gtaatattgt ggtgcaacta tgcctgcttt tgatatatag ctcatattta
    20521 tgcctttgta tgaaaggtag gcaatatcaa gtgcccggtc aggcaaaacc gttaattcaa
    20581 gaccgctgcc attctttata tctaccgctc tcactccatt agcttttcca tctgccagac
    20641 ggtaattttt tacgccaaat aactgggatg tatctcctat gtatttaagg atctctctcc
    20701 ggtctaaagt ccctctttct aactcatttc tttcttcatt ttttcccatg cttttccctc
    20761 ctgttgactt ttatttatct tagtactact ttgtttatgc tctcttggtt ccatattcca
    20821 gatcaaaata aagcagggca atcaatcttt gttgcataag agtaagtatt gaagacaaaa
    20881 atgtcacaac aaaacttaat ataagattgt tcccatatac tgaagctaaa ataataataa
    20941 aaaatattgg tagaagtagt gtaagattaa aaacaatgaa tatggcaaat atctccattt
    21001 tccttcttgc agttaagttt ttacttgcat taaatgcatc cgtaactcta atgtccttat
    21061 caagcaaata gcatatatta aacataaaca tgaggtaaaa aattacccct ggtattatca
    21121 aaagaaaaaa tcctgttaaa attatagcta taaaagcgat gtaagcagcc aatatcttgc
    21181 tcagctttct aaatacctgt cctaagcatt gtttcagaga gcattctcca ccccttaact
    21241 cagtcatata tgaatataaa tatactacgg acgccaggta aaggataaat acacttgcaa
    21301 tattgtacat tgcgcttaag tatatatcat ttgcggtttt ttctgatatg agtgtacttg
    21361 caaaacctgt gaaaagtatt ataacaaata atacaggagt tattctttca tttttcatct
    21421 cataaaactt cagtgcattt attaaataat attttgccat tcttaaattt gatggttttt
    21481 tattaggttc catacctttt acctccaatt atgcagatcc tattcagcct attcttgcct
    21541 ttacatatac tttaatatat attgaaaaat tatgcaatta ttttcatgtc ttatggggtg
    21601 catcagctat ctttttggtg tataatattt tcggacatgt ttataaattc agtatacact
    21661 atagatgctg cttgtaagta attattaata tacacatacg gggtgatgtt atttgaagca
    21721 tattgtaagc gtcagtttag gatcttctac aagagatcat aaggttgagc tggaattgct
    21781 cggtgataag tacattgttg aaagaatcgg aatggatgga agttacgata aatttctgga
    21841 aactcttaaa gatttagatg gtaaagttga tgctatagga atgggcggca ttggcatata
    21901 ccttcctgtt aaaaatcata cttatttaat taagagcgcc ttacctctaa tgcatgcagt
    21961 taaaaaaaca cctgttgccg atggatcata tttgagaaat gctttagagc cttacgcagc
    22021 caattttgtt caaaaaaaag gtataattaa ctttgcaggt aaaaaagtta tggttacatg
    22081 tgcagttgac cgctatgctc tgtctgaagc ttttgtaaat cttggctgca gtattacctg
    22141 tgctgatttg gtttttactc ttggcatacc ttttgtagta agatcactta acggattgga
    22201 caggcttgcg aagcttttcg cacctgccgt tacaaaactg ccttttgata tgctgtatcc
    22261 aaccggtcaa agggaagaaa atgttgaaaa tcccattaaa ttcgcaaaat attatagaga
    22321 ggctgatatt atttcaggtg atttcaacta tataaagaga tatatgcctg atgatttaac
    22381 cggtaaagtt attatcacta atactataac aaaggaaaat gtagaagagc tcaagtcaag
    22441 aggtgtatcc acactggtta caacaactcc tgaatttaat ggacggtctt ttggcaccaa
    22501 cgtaatagag gccattatga taaccatact tggaaaacct gttgatatga ttacagtaga
    22561 agattatata gctacatccg aaagactggg atttgaaccg agagtggtta ggttttgatt
    22621 agaggttaga ggttagaggt tagaagtgag aagttagagt tttggggtca aaagtgagtg
    22681 ggggtgtaac ccccactcaa tcctcgaacg aagcgagttt gccactttgt tgaatctgtt
    22741 caatttcccg cttcccactt cctactatcc actatccact acccacttat atgctaagtt
    22801 attaattact tttcaggata gttttccttt ggtgcataat gagtatgcaa tagctcatgt
    22861 gctttatgac ttcccggttc cccaaagaag tccttataaa gcttttgaac cttctcattc
    22921 tcatgggact ttcttattgg cagatctcta tcttcttcgt agatagcttt tgccctctct
    22981 tttctgatat ctatccagct tctaacttct gaaggctgta tgggctgtcc gccgccatta
    23041 acacatcctc cgggacatgc cattatttct atgaaatgat agtctgcctc tccggccttt
    23101 atcctatcca aaagctttct cgcgtttgca aggccatgag caacagcagc tttgaccttc
    23161 attccaccta catcaacctg agcttccttt ataccctcta cgcctcttac atcaagatac
    23221 tcaatatctt catgagattt accggttaat acatctgcaa ctgtccttaa ggcagcttcc
    23281 ataactccgc ctgttgctcc aaaaataaca ccggcaccgg tagcatttcc cataggatca
    23341 tcaaaatcag agtctggtaa atctttgaag tccattccgg tttctcttat cattcttccc
    23401 aattctctgg ttgtcaggac aacatccaca tctgcgtagc ctgttgatga caattccggt
    23461 ctctgggcct caaatttctt ggcagtacat ggcataattg aaaccacaaa tatttttgaa
    23521 gggtcaatgc ccatcttttc agcatagtat gatttcaata aagctccaaa catttcatga
    23581 ggtgatttgc agctggataa attatccagg aaatcagggt aattatgctc acagaatttt
    23641 atccatccag gactgcagga agtaatcaac ggaagttttc ctccgttttt aattctattg
    23701 attaattcgg ttccttcttc cataatagtt aaatctgctg ctgtatcagt atcaaatacc
    23761 ttgtcaaacc caagacgttt caatgcagat accattttac cggtaaccct tgaacctata
    23821 ggcattccaa attcttcacc cagagccacc cttactgcag gtgcagtctg gactattaca
    23881 tgcagatcct tgtttgccag tgcctgccat accttttccg tatcatcttt ttccctgagg
    23941 gctccaactg gacatacatt tatacactgt ccgcacattg tacatggaac ttcattcagt
    24001 gacatattga atgctgatga aacaatagta tcgaatcctc tttcattagt atcgataact
    24061 gccacctttt gtatattttt acacatactt acgcagcgtc tgcacaatat acatttatta
    24121 gggtctctca cgattgaagg agaaaaatca tccagaggca ttttgtgggt ttctccgtcg
    24181 aaccttatat cactaatatg caattcctca gcaagctttt gaagctcaca attcctgctt
    24241 cttacgcaag taaggcactt cttttcatgg tttgaaagaa taagctccag agttaccttt
    24301 cttgcctctc ttattgcagg gctctgtgtc tttacaacca gaccttctga aacaggatat
    24361 acacatgcag cctgtaaact tctggcaccc tttatttcta ctacgcacat tctgcatgca
    24421 cctatttcat ttatgtcttt taagaaacag agtgtgggta ttgatatatt tgcctgttta
    24481 gcagcttcaa gcactgtaaa attctttggc acttgaatat ttctatcgtc aattgtaata
    24541 tttaccattt ccatatttgg tacctccttt cattacgcgt tcttaaatat tgccttaaat
    24601 gggcattttt gcatacatgc tccgcatttt atacatttat cttcgtttat aacatacggt
    24661 accccttttt gtccgcttat gcagccaaca gggcagtttc ttgcacatat tccgcagctc
    24721 ttgcagcggt ctgcctgtat tacataatgc atcatggatt tacaaacacc tgcaggacac
    24781 ttcttatctt ttacatgagc ctcatactca tccctaaagt accttaatgt actcagtaca
    24841 gggtttggag ctgtctggcc aagtccgcaa agtgccgatg ctttaatatt ctttgcaagt
    24901 atttccagtt tctctatatc cccttcttct cctttgccgc ctgtaatcct ttcaaggatc
    24961 tcaagcattc ttttagtccc tatacggcat ggaggacatt ttccgcagga ttcatccaca
    25021 gtaaactgca ggaagaactt ggcaatatct accatgcaat tatcttcatc cattacgatc
    25081 agcccaccag aacccatcat tgaaccaagt tgaataagag tatcatagtc aataggtgtg
    25141 tcaatatgac ttgcaggaat acatccaccg gaaggtccgc ctgtttgtgc cgctttaaac
    25201 ttctttccgt tgggaatacc tccgcctata tcatatacaa cctccctgag ggttgtaccc
    25261 attgggatct ccactaaacc tgtattatta atttttcctc ctacagcaaa cacttttgta
    25321 cctttgcttt tttcagtacc aatgcttgaa aaccattccg gtcccttt
//
LOCUS       DAOSDD010000031        24627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_904_length_24627_cov_4.951693, whole genome shotgun sequence.
ACCESSION   DAOSDD010000031 DAOSDD010000000
VERSION     DAOSDD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24627
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_24627_cov_4.951693"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..935
                     /gene="sdaAB"
                     /locus_tag="PK733_04605"
     CDS             270..935
                     /gene="sdaAB"
                     /locus_tag="PK733_04605"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOJ09856.1"
                     /translation="MNIFDIIGPIMVGPSSSHTAGAARIAAVTRKLLGEDVKNASIKL
                     HGSFATTYKGHGTDRAIVGGLLGYKVDDPAIRESFKHAQLSGLTFNFEKEDLGDVHPN
                     TALIEAIGVSGKKIKVMGCSVGGGRIMIKRIDNFPVEFTGQYYTLVIEYKDKPGVIAS
                     VTSILGRNSINIAEMRVYRSYRGGTAVMIIEADQVIEKSLIDQVKNIQDVYNTVGIDA
                     VEV"
     gene            1031..1906
                     /gene="sdaAA"
                     /locus_tag="PK733_04610"
     CDS             1031..1906
                     /gene="sdaAA"
                     /locus_tag="PK733_04610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOJ09857.1"
                     /translation="MLTYKSINEIVEAAEKEQKNISQIVIYEQSLQLNSSIDELYLKM
                     SENLKVMKAAVEEGLASSQKSISGLSGGDAVKLKKHVDSGYNIGGKFFCNMLQKAIAI
                     AETNAHMGRIVAAPTAGSCGIIPAVLLTIGEENDISEDKIIMSLFTSAAIGMVIANRA
                     SISGAEGGCQAECGSASAMAAGAAVEALGGTPKMISSACSIALKNVLGLVCDPVAGLV
                     EVPCIKRNAMGAANALAAAEMALAGIESVIPADEVIDAMRSIGNLMPVILKETAEGGL
                     AASPTARKLAGKILC"
     gene            complement(2017..2847)
                     /locus_tag="PK733_04615"
     CDS             complement(2017..2847)
                     /locus_tag="PK733_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09858.1"
                     /translation="METSFFKMFAGDSLNLPFLFTTVGICSNQDYISRPNGHPDYHWL
                     HCVQGKGILLVNNNEHIIHEKSGFFFHPGVAHQYYALEEPWETHWITFNGYAVSPLMQ
                     LLGLGKCEVFYILDMLKVEKMFDDIYLSAQSSSVFRGYECSYLLYKFIIELRNCLDTD
                     FSTPRYLKYKQLQPVISFMEDNYNMDISLQDMADIMNVTPRHFCRLFKEAFNLSPFKY
                     LIAYRIKKAKELLVLPDNPTIKEVALKTGYNDISYFCKIFKGYEGLTPLEFKRMYRSI
                     "
     gene            3155..6418
                     /locus_tag="PK733_04620"
     CDS             3155..6418
                     /locus_tag="PK733_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ09859.1"
                     /translation="MLNIEKYWEDLNTLHVNREKPRAYYIPYADAETALSKKRGKSPF
                     YQTLNGNWKFKYYTSVKLVEEGFYERDADVSMWDNLIVPSCWQVKGYDLCHYTNVNYP
                     FPCDPPFVPNENPAGTYVRDFNISGNWENKEKYVVFEGVNSCFYLWVNGIFAGYSQGS
                     RVPAEFNITPYISKGRNRITVMVLKWCDGSYIEDQDIWRFSGIFRDTYLLARDKIHIR
                     DVFVKQELSEDLKSALLKCEIDTAIADCCEISAELKDSEGKIVSESKVILDNNGDSGR
                     DNIANGRTTIAMKVNEPVLWNAEAPYLYKLFIYCGKEVLLFNVGFRKIQVKDSVFMVN
                     GKAIKLKGVNRHDSHPQLGQTVPLNHMIKDLMIMKRHNINTIRTSHYPNNPRFLELCD
                     LYGFYVIDEADLECHGVAPAGDFHMLSKNSQWEKSFVDRAERMVERDKNHACVIIWSM
                     GNESGYDVNHIAMAKWTKNRDASRLVHYEGAASIYKGNPDTEYLDINSRMYPAIEYVE
                     QYALNEENNKPLFLCEYSHAMGNGPGDLKDYWDIIYKYPKLMGGCVWEWCDHSIKTVT
                     SDGKEFYAYGGDFGDKPNDGNFCLDGLVYPDRRPHIGLLELKKVIAPIKIEPQDLEKG
                     IIKVINLYDFIDLSHVSLAWKLEKDGCTVEQGEIPAMETAPHQSQVFQLSYKLPGKLP
                     DKLPGFNMLNESASEQQDEIRPKQMHELPNKLNNRYFLTISCVQKKDTPWAERGYEIT
                     FEQFELLVAAEVERTDKPEPCGTANPGGTSDIMKSHEACSDIKKPETCKFYMPEFHIP
                     EFRIEQKGYLITIEGFDFYYIFDMYEGTFVKISRNYVDMIKEPLKFNMWRAPTDNDRN
                     IKHKWMAEGYDRAGMRVYESKITNLTDTFIEISVDFSLGGYIKLPVLHGQAVWVVDGT
                     GEIYLKLNAEVRENLVFLPRFGLQLTMPQGNEEVEYFGYGPHDSYIDKRQSVRKGKYL
                     TTVDNMFENYLMPQENGSRFGTEWAIVSNEQGMGLKFTGCPEFSFNAAHYSPEDITAA
                     RHPHELKKRKETIINVDYKMSGVGSNSCGPELLGKYRFDEKEFEFRLKFIPVFKEDE"
     gene            6886..8169
                     /locus_tag="PK733_04625"
     CDS             6886..8169
                     /locus_tag="PK733_04625"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOJ09860.1"
                     /translation="MVPEGLKITVIGGGSTYTPELVNGFIKSGNELPISEFCLMDIDA
                     RKLNILGGITRRMFAEAGINCTVTCTENREIALRNSDYVVSQIRVGQMDARIKDEKIP
                     LEYDLIGQETTGIGGFMKAVRTIPVMLKIAREMEKYCPEAWLINFSNPSGLIAETLLN
                     NTNIKCIGLCNVPLGMLKKAYKIMQGEPDNYKVDYIGLNHLAWITSVHYKNRNILGDF
                     IEKTCIKMGKYYDKIDILLKNIKAVPCGYLYYYYYRDYYLKKLKAQDKTRGEVCLEIE
                     NELLHLYNQPNIKQKPELLDKRGGGGYAEALISLLSSIYNNKNEIHIVNVKNQGALDF
                     MGYDDVVEVACYVSRNSITPIPMNGFKNEHIKDMMRTVKAYEKHAVNAGLYGDYSEAL
                     KALFIHPLVGDYEKAKAALDALLKAHREYLPQFNI"
     gene            8215..9210
                     /locus_tag="PK733_04630"
     CDS             8215..9210
                     /locus_tag="PK733_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ09861.1"
                     /translation="MKKYVLGIDGGSTKSQYGLFDIEGNMINFLQGGCVNHAHFTDKY
                     DGMERELKKCITEITNKADIDINSIEFCFLGISGADSKNDHIEISRKVSCIGIGKFEV
                     CNDAFLGILAGSPKKYGVCSVNGTGTNCAAIDRKRQKLLIGGWPYGGGNYLIHLMLKR
                     VYDHYFRCGKYTIMAEKLFMELGLSCEDEMEEIVYKKVYGRQIDRSRLTSIPFYAVSK
                     NDEVAKEILDIMALDAARSVAGAVNRLDFSQEKEVDVILAGSIYVKGEDPYLINAFKR
                     YTKELLNAEINFILLDVPPVAGAVIQAVEIVTGLCDKVIYTKIIKTLSTLCEDNK"
     gene            9276..10091
                     /locus_tag="PK733_04635"
     CDS             9276..10091
                     /locus_tag="PK733_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ09862.1"
                     /translation="MYWEELTSREFEKARKTTKGTCIIPFGVIEKHGTHLPLNTDMLV
                     GRKVVSEVVKIEPAVIFPYYYFGQIAEAMHCPGTISIEPELAYALLKNVCKEIARNGF
                     KKIIILNSHGGNIRFIDYFIQTAIYEKKDYVIYGVSALLNPKELMEIRTIYNCDKVGG
                     HGGNHETSIVMLERPDLVHMDCIEEEGLKNYGRINHISREMGIHTAMWWYADHPTHFS
                     GVVENSSAEAGEKACRLITERVAELVRAIKEDTIAKQLQDEFFAKSETLGSRD"
     gene            10104..11147
                     /locus_tag="PK733_04640"
     CDS             10104..11147
                     /locus_tag="PK733_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09863.1"
                     /translation="MMSLFKHDFSRLKKVLLREGEPDRVPFFEIIVDGKVIEYLTGKA
                     PSISVNAEFYIKHGYDYVMSSFGIPYRMEKLPAADTSLELTKERRMFINEQKGIIGNR
                     YEFDTYKWPVVDTEILHKIKKTIEILPDKMKTVILAGDGILESAMALMGYTQLSFMIY
                     DDPLLINNLFEKLGKDLLKAFELCMKNVNVKSIGAIVIGDDLGFNTSTIISPDDIRKY
                     IFPWHKKIVELAHRYDLPVILHSCGKLDEIMEDIIDIGYNAKHSFEDNILPVTEAKKK
                     YGDRIAIMGGVDINVLSTATEKETRQYVENILDKCMPGGGYALGSGNSITNYISVKNY
                     LAMLDEGRKKGVY"
     gene            11214..12455
                     /locus_tag="PK733_04645"
     CDS             11214..12455
                     /locus_tag="PK733_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09864.1"
                     /translation="MNELLFGYFDTDITPSISMGLEGYPYRLNHGFENGGVIDRIYAC
                     ALCLKSGSEALLIITVDLCDLSFETADDIRNAISLKTGLPRRNIMLIATHTHSAPVTI
                     CQNVKNKEAIDKIEDYLKELKNKIISISSQACARRYSAKLSISTLKTCLGYNRRYISV
                     DEKGNSNCKMLFNLWQNHGHDTTGEVDSEIPIIMIEKEDDENRDNFLTPTGINRVIIV
                     NAPYHPVVMGENNNYISADYPGAIRKCIENELMDGTKVISITGASGNTQPFISCVNNL
                     RAIRIFGNAIGYGVITALAGRKYIPVDGIQGIEEEFDYNNPETNRIRTQVFRIGNIGI
                     AGISGDCFNELGTFIRANSGIRQILIATHANGRSGYIPTPESFNIGGYEIDIAIKRGY
                     DTDVFEKVVNQVLKNLKTLRE"
     gene            12518..13897
                     /locus_tag="PK733_04650"
     CDS             12518..13897
                     /locus_tag="PK733_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOJ09865.1"
                     /translation="MRNGAIIEKGPMNWQIIQHKNGIAVLYLSGRWITEKNMIQPRVY
                     AKLFNEFTGEGITQWIECDTFDDGTWKVSLDNIPKGGPYRIETCLYDKADTTIGIEWA
                     LRGDMIFHIGVGDLFVIAGQSNAAGYGKEPVYDPPEIGIHIYKNNGKWDVATHPLNDS
                     TDIIYSNMENSNPGHSPFISFAKTLKNELGYPIGLVQASLGATSISRWNPGEDGTLYR
                     NMLEKIQSVGGTVKGILWAQGGTDANRGYSKNYIERFTNMVRCLRKDLNDNLLPIFTM
                     QSGRHVKFQDKNNDRDWAEVREAQRQASKKLDNVYTISSLNSMLSDYVHMNSFSNIML
                     GERYAKMVLTKLYGKSFLCSAPDISRAKKTDENQVTLYFENVVGKLYVFDLAANELPF
                     TVEDEKGKYSILSYEIFDKDKIILNVDRTLENNSKVHCAFERNPGQFVPIDRESCIPM
                     LAFYGVEIE"
     gene            14004..15068
                     /locus_tag="PK733_04655"
     CDS             14004..15068
                     /locus_tag="PK733_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09866.1"
                     /translation="MELKMNSRGRVLTALKRGIPDAVPYMYNTMDKELQEKIIGKEIS
                     LPTETGMNSLGYLSRPGDEAYVSPILTLVPEVADLLGLDAIGIKIFPPLFVDGGIRDG
                     RAMIKKGLLNTREALEKCKMPDPDDDKLYKSIEKLLDVCAGDRAIYAKIRLGASSTLM
                     SMDMMGFSYNLHDDPELVSEVLMMYCSWSQKICRNLCEMGFDFIWCFDDIAFKTAPMF
                     SPDVLNNIFLPILMKTTSSINKPWVFHSDGNVIPFLDDLLKLDMDGLHPLEPGAMDLD
                     SLKSKYGSKLCLIGNIDIDKTLTRGTIQDVDNEVRERICLLGKGGGYIISDSNSVPAY
                     CKAENVIAMSNAVSKYRYIY"
     gene            15249..16196
                     /locus_tag="PK733_04660"
     CDS             15249..16196
                     /locus_tag="PK733_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09867.1"
                     /translation="MNADIKNKKTRINIMVRDFKKNKTLLLLAFPMILYVFIFKYIPM
                     FGIIIAFKNFKYNLGFFKSPWVGFKNFQFFFTSPDAWLVTRNTVGYNLVFITLGIIVQ
                     VGVAILLFEIASRKLHKIYQTLMFIPHLLSWVVVAYMAYAFLSPSGGMLNRWLSFFNI
                     EPVDWYITPKPWIFIFPIANMWHGLGMGALMYYAKLMGINNEYFEAAEIEGASKWQIT
                     KNITLPFLYSLIIMLTILAIGRIFDGDFGLFYQLPMGSPMLRKTTDIIDTYVYRALVD
                     VGNVGIASALGLYRSLMGMILVIVTNMIVRKIDEENALF"
     gene            16234..17133
                     /locus_tag="PK733_04665"
     CDS             16234..17133
                     /locus_tag="PK733_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09868.1"
                     /translation="MSISAIKRNTVSQIIINIFFILLSVSFITPLFAIISISLTSERD
                     VVNYGYSLIPPNIDFSAYKFILSSPSTILSAYKVTAITSFIGVLSSLLISSMCAFSLS
                     RKDFKYRRQLTFYFFFTMLFNGGLVPYYILMTKYLSLRNTYLALILPLIGNIWHMLIL
                     RTFFQSLMVEIIESATIDGASDVLIYAYIALPLSTPALATIGLFQLLSFWNSWYPALL
                     FITKKSLYPLQYLLQSMLRNAQEIIRNMEQMSFIAPEELKNVPTESTRMAMCIIAIGP
                     ILFIFPFFQKYFVKGLTIGAVKG"
     gene            17217..18737
                     /locus_tag="PK733_04670"
     CDS             17217..18737
                     /locus_tag="PK733_04670"
                     /inference="COORDINATES: protein motif:HMM:NF023435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ09869.1"
                     /translation="MSKKLSKDGFLMGKYVRILCILIVVIMLFVLSVGCVKVKTEGEK
                     TESKVTTESTETKSEPEPLKPVKLLWYVRAQTPNNEKNVLEAANKIIGEKINATLDLQ
                     FINSGDFNEKMRMIMSAQEEYDLAYTANWANTFSNNVSNGAYIELDDLLKERKALYNI
                     IPEKIWEALKVNGKTYGVPNYQIMYNETGFWFKKDLIDKYNIDLSKVKKMEDLTPIYQ
                     TIKDNEPDVIPLRAGNASTVFMGDYHPEIDNLLAVNPKTWEVYDMKPTLMDRYKLMRE
                     WYQKGFFPSDVATLKDERPLILEGKIFSRIVRIKPGVEGELQASYGYDFVTISASDKV
                     LNYSGAASALTGISTTSKNPERAMMLLELTNTDKELLNLLTYGIEGQDYVKIGTDKIE
                     KKPDSYTFYAWQLGNQFIAYTLKGQDDSVWEETRKCNEQSIIDPLFGFTFDRTPVENE
                     VAQLKAIRTNFDDIMNNGLTDPEEAMADRTQQMKAAGIDKVIAEMKKQVDAWRQSK"
     gene            18987..20789
                     /locus_tag="PK733_04675"
     CDS             18987..20789
                     /locus_tag="PK733_04675"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ09870.1"
                     /translation="MKSIQKKLLIYISLVSVFPIIILGTVSYYVSSNALEKKINESSM
                     SRLHQIVKNLENKNTKVENFLNMLSASSEIGNILRNYDQNTKPVDYYLAANKFRQTFL
                     SFFYNDKSIDSGFILSNKGFTYEYNCYIEKNKSLKLLDWYDKTTENKVDNTWVSYIEK
                     PNDIIKSFTKADDIILVGTNVRDINYASDLSVLGIACIFLNKSFFSSIYEGVDELPDT
                     NTVIIDDKGNIVITSDKAAFYSQLDNISGYSFAKTVLENNKGLFRCQVNKSDLVVSYM
                     TLNTNGWKVLQVTPYSYYIREITSIGWISILLAAICLCIIYLLAFFVSKSISRPLLNI
                     VKMMDKVTNGDFQVTVPVTRNDEIGIICAGFNNMVKSIGELFSALKEEEREKRKAEIM
                     KLQYQINPHFLYNTLASIRLMASLIKADEIARIIKILSRLLRNTISKADQLTTIYEEF
                     ANAMDYIEIQQIRYDNKIFVEKDIEKEILPLKIKGMLLQPILENSIMHGLAEKLNNGS
                     KAAYLKISGYKKQNNIYFEVFDNGKGMTPEQIKNVFEKNDSLEGQIGVKNIHNRIRLL
                     YGDEYGASIESVRGEWTKVVLSLPYENEEVDKYV"
     gene            20782..22383
                     /locus_tag="PK733_04680"
     CDS             20782..22383
                     /locus_tag="PK733_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09871.1"
                     /translation="MFSILIVDDETLIRKALKTLINWEQLGFKLIADVYNGIEALKII
                     DSCHVDIVLTDLKMPEMDGLELMRNVIKKYPHIKFIALSAYDEFHMVSEAFKLGAKEY
                     FLKSEMEEDDIVKAIQNIKTEIERDKLEELHKKKIRDEMKSSGNALKQEILLDIVMRN
                     RIATDRELKLLNDRYLPAFNPENMRVMHINIGKYLKLEDKIEVYNYENIKNNIYNAIK
                     QKIEEYTNVEILYRYPDEIILICSFSNLVSKRQLINEFLGFFSDINNYIYKKYNLKIT
                     AGLSGYGRGYNSLNSLYIQALTASEHSFIFGKGKLLIYSNLPINNKSVNIEKHQKPMA
                     FRDLLKTMDADRISRGYVMFVQKPEDASCKNIIDIKIMFQQYYFIVMEFTHHVNIIDN
                     INELAEEYEKYLRDMGDLLELNNWLKRLMQKIAETLENTSNLILSLKKYIHQNYSQNI
                     VLSDVAEKYSISTCYLSRVFAEEVGCNFAQYLSKVRIEAAIDLLINTNLKIYEIAERV
                     GYSNSEHFSRSFKKIIGKSPKTYSR"
     gene            22564..24279
                     /locus_tag="PK733_04685"
     CDS             22564..24279
                     /locus_tag="PK733_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09872.1"
                     /translation="MNRNVFSRYMALFATTIAILIFYSSFKLIEKGKAHSSYIIQNNE
                     EITQNNNNKNIGIEEKQREYKISWASWTPIKFKENNSWGEKCIEEYFPDVDIQFIHFD
                     KSTWEIQLSALIASGTYPDAIRLKDRTMIYTYVRQGVLAKAPYSVVKENAGRYIEAME
                     ETGVDHWLTTLINGTNYGIPVLNVRSGSDYCNVWRSDWLESTSIYKIPDTLEEYEEAF
                     RKFTLEDPDENGIDDTFAYAPIGTEKDIQYQFNTIFAAHGVFPFTWMVMEDGTYKYGL
                     VMEEAREAMRRLNSWYKNGYIDPDFMKNDAEIAKAKLLNGKSGFREWRGIWEWRPPRD
                     GGNAGYEYAEALKLNPNCEFKMGYPPEGSSGKRGLYDWGNVTGAVCFGKHLDNELQKM
                     GKIIQILELFATNEEAAIKLEYGIEGEHYIYDSVTGSKNRLPPYDSGNEGWTLLSPNG
                     LFGWSRIREVSDALQDNYIVEDFGWNSKNTLLAGINYIPYVNQFVDLEMLKYQIQARE
                     IADIWELEFITGSKSLDDDWYEFIKAWKESGGEIVTQQVNMMARKAIEENLQNIESSI
                     NAITK"
BASE COUNT         8687 a         3494 c         5037 g         7409 t
ORIGIN      
        1 aatttttctt tgccttaaac ataacaatcc gtaagctttg ttcaaatttt atacttggaa
       61 aatcgtacgc tgccatattt tgtatcatag aaagaaaatg cgccacctgc aggcttatta
      121 ggttgcggct atacaacctg atatgttata ataaatgcag ctaaggtaat aaaacctggc
      181 ggttttgtct ttatgcattt attgaaacgg tgtgaagcaa tccgattggc ggattgcact
      241 gatgttataa aaattgggtg atttagacca tgaatatttt tgatattata ggccctataa
      301 tggtggggcc atcaagttca catacagcag gtgcggcaag gattgcagct gttacaagga
      361 agctcctggg agaggatgta aaaaatgcat ctataaagct tcacggctct tttgccacta
      421 cgtataaagg ccatggtact gacagggcaa ttgtgggagg tcttcttgga tacaaggttg
      481 atgaccctgc tataagagag agcttcaaac atgctcagct ttcagggctt acttttaatt
      541 ttgaaaaaga agatttaggg gatgtccatc ctaacactgc acttattgag gctataggtg
      601 tttcaggtaa aaaaataaag gtaatgggat gttctgtggg cggaggccgc ataatgataa
      661 agcgcataga caattttccg gttgaattta cgggacagta ttataccctt gtaatagagt
      721 ataaagataa accgggcgtg attgcatcag ttacttcaat tctgggacga aattccatta
      781 acattgctga aatgagagtt taccgttcat acaggggcgg gacggctgta atgataatag
      841 aagctgatca ggttatagaa aagagtttaa tagatcaggt aaaaaacata caggatgtat
      901 ataatacagt aggtattgat gctgttgagg tttgacattt aacgcatgat tagccagtaa
      961 ttagcggtta ttaaccagta attaatccgc acttagcggg aaattaagca gcatttaaca
     1021 agggggagct atgcttacat ataagtcaat taatgaaatt gtagaagcag ctgaaaaaga
     1081 gcaaaagaat atttcgcaga tagttattta tgaacagtct ctgcaactta acagcagtat
     1141 tgatgaatta tacctcaaga tgtcagaaaa tctgaaagtt atgaaagcag ctgtagaaga
     1201 aggccttgcg tcttcacaaa agtctataag cggtctaagc ggcggtgatg cagtcaagct
     1261 aaaaaagcat gttgattcag gatataatat aggcggaaaa tttttctgca atatgctgca
     1321 aaaggctatt gccattgctg aaaccaacgc acatatggga agaattgttg ctgctcctac
     1381 agccggttcc tgcggaataa tccctgctgt gcttcttaca attggtgaag aaaatgatat
     1441 atctgaagat aaaataataa tgagtttgtt tacatcagca gcgataggaa tggttatcgc
     1501 aaacagagca tctatatcgg gagctgaagg agggtgtcag gcagagtgcg gcagtgcttc
     1561 tgctatggct gctggtgcag ctgttgaagc tcttgggggt accccaaaaa tgatttcaag
     1621 tgcatgttcc atagcattaa aaaatgttct tggactcgta tgtgatcctg tagcagggct
     1681 ggtagaagtt ccctgcataa aacgtaatgc tatgggagct gcaaatgcac ttgcggcggc
     1741 tgagatggcc ctggcaggaa ttgaaagtgt tataccggca gatgaggtta tagatgccat
     1801 gcgctccatt ggaaatctta tgcctgtaat attaaaggaa acagcagaag gaggacttgc
     1861 ggcttcaccc accgccagaa agctggctgg aaagatcctt tgttgaatca tataattgta
     1921 taatagattt atacagattt ttaccgactt atacggattc atattaatcc acactcatct
     1981 atatggctct atacattgta cagggttcta tatattttat atagacctat acattctttt
     2041 aaactctaaa ggggttaagc cttcataccc tttaaaaatt ttacaaaaat agctgatatc
     2101 attataacct gtttttaaag caacttcctt tatagtgggg ttatcaggta atacgaggag
     2161 ttcttttgct ttttttattc tatatgctat aaggtattta aagggactca aattaaacgc
     2221 ttccttgaac agcctgcaaa agtggcgggg tgtaacattc ataatatcag ccatgtcctg
     2281 aagtgatata tccatattgt agttatcttc cataaatgaa ataacaggtt gaagctgctt
     2341 gtatttcaaa tatctcggcg tggaaaaatc tgtatccaga cagtttctca attctataat
     2401 aaacttataa agcaggtagg aacattcata acccctgaaa acactgcttg attgggcaga
     2461 caaatagata tcatcaaaca ttttctcaac cttgagcata tctagtatat aaaacacctc
     2521 acatttacca agaccaagca attgcataag aggagatact gcatatccgt taaatgtaat
     2581 ccaatgagtc tcccatggtt cttccaatgc ataatattga tgagctactc ccggatgaaa
     2641 gaagaaacct gacttttcgt gaattatgtg ctcattatta ttaacaagta gaattccttt
     2701 tccctgtaca caatgaagcc agtgataatc aggatgcccg ttgggcctgg atatataatc
     2761 ctgattagag cagattccca ctgttgtaaa taaaaaaggc aaatttagcg aatcacctgc
     2821 aaacattttg aaaaatgatg tttccatata attacctccg attttaattt aggcagttat
     2881 gaggacagtt cttcccaaat agggtcagtc ctctaataat attatactat taatatgtct
     2941 ataatgccat ataaatgaca atatactatt gttgaaagtt gtatacatgt aatctataat
     3001 aataatataa ttacattact gcagaaaagc aatgcatgat aaaataatca tatataatgc
     3061 ttatatagaa tgctcataca ttatacttga tgctcgcact taatacatat gcaatatgta
     3121 atataacaaa attaataaat tccggggtga tataatgtta aatatcgaaa aatactggga
     3181 ggatttgaat acacttcatg taaacaggga aaaaccaaga gcatattaca taccgtatgc
     3241 tgatgctgaa acagcccttt caaaaaaacg tgggaaatct cctttttatc aaactctgaa
     3301 tggaaactgg aaattcaaat attatacaag tgtcaaactt gtggaagagg gcttttatga
     3361 aagagatgct gatgtaagca tgtgggataa tttgattgtt ccttcatgct ggcaggtaaa
     3421 aggatacgac ttgtgtcatt atactaatgt taactatccc ttcccctgtg atccgccctt
     3481 tgtgccaaac gaaaaccctg caggaacgta tgtgagagat tttaacatat caggaaactg
     3541 ggaaaacaaa gagaaatacg tggttttcga aggcgtaaat tcctgtttct acctgtgggt
     3601 taatggcatt tttgcaggtt atagtcaggg aagcagagta cctgctgaat ttaatataac
     3661 tccatacata agcaagggga gaaacagaat aaccgttatg gtgcttaaat ggtgtgacgg
     3721 aagctatatt gaagatcagg atatatggcg gttttcgggt attttcagag atacatattt
     3781 acttgcaaga gataagatac atataagaga tgtctttgta aaacaggagc tttcagaaga
     3841 tttaaaaagc gctttattaa agtgtgagat agataccgca attgcagact gctgtgaaat
     3901 tagtgctgaa ttgaaagata gtgaggggaa aatagtttct gaaagcaaag ttatattgga
     3961 taataatggt gacagtggca gggataatat tgccaatggc aggactacta ttgcaatgaa
     4021 agtaaatgag cctgtactct ggaatgccga agctccatat ttgtataaat tgtttattta
     4081 ctgcggaaaa gaggttcttc tttttaatgt aggctttaga aaaattcaag taaaagacag
     4141 tgtgtttatg gtaaatggaa aagctattaa attaaaggga gtcaaccgtc atgattctca
     4201 tccccagctt gggcaaactg tccctttaaa tcatatgata aaagatctta tgattatgaa
     4261 gcggcacaat attaatacca taagaacatc tcattatcca aacaaccctc gttttcttga
     4321 attgtgcgat ttgtacggct tctatgtgat agatgaagct gaccttgaat gccatggagt
     4381 tgcaccagca ggggattttc atatgctgtc gaaaaattct caatgggaaa agtcttttgt
     4441 agatagagct gaaagaatgg ttgaaagaga taagaaccat gcttgtgtta ttatatggtc
     4501 tatgggaaat gaatcaggat atgatgtgaa ccatattgct atggccaagt ggactaaaaa
     4561 cagagatgca tcaagattgg ttcattatga aggcgcagca tcaatttaca aaggcaatcc
     4621 tgatacagaa taccttgaca ttaacagcag aatgtatcct gctattgaat atgttgagca
     4681 atatgcgtta aatgaggaaa ataataaacc tttgttttta tgtgaataca gccatgccat
     4741 gggaaatggt ccaggagact taaaagatta ctgggacatt atatataagt acccgaagct
     4801 tatgggtggc tgtgtgtggg agtggtgtga ccacagcatt aaaactgtaa cttctgatgg
     4861 caaagagttc tatgcttatg gaggagattt cggagacaaa ccaaatgatg ggaacttctg
     4921 tctggatggt ctggtatatc cggatagaag acctcatatt gggctacttg agttaaagaa
     4981 ggttatagct cctattaaaa tagagccaca agatttggaa aagggaataa taaaagtcat
     5041 taatttatat gattttatag atttgtccca tgtttccctt gcatggaagc ttgaaaagga
     5101 tggttgtact gtggaacaag gtgagattcc tgctatggag acagctcctc atcaatcgca
     5161 ggtatttcaa ctttcatata aattaccggg taaattaccg gataaattac caggatttaa
     5221 tatgcttaat gagtctgcaa gtgaacaaca agatgagatt agacctaaac aaatgcatga
     5281 gctgccaaac aaactaaaca acagatattt tctcacgata agctgcgttc agaaaaagga
     5341 tacaccctgg gccgaaaggg gctatgaaat tacttttgaa cagtttgaac tgctcgtggc
     5401 agcagaagtt gagcgtactg ataaacctga accctgtgga accgcgaatc ctggtggtac
     5461 aagtgatatc atgaagtccc atgaagcttg cagtgacatt aaaaagccgg aaacatgcaa
     5521 attttacatg cctgaattcc atatacctga atttagaatt gaacagaaag gatatctgat
     5581 cacaatagag ggttttgact tctactacat ttttgatatg tatgaaggga cctttgtaaa
     5641 aatttccaga aactatgtag atatgataaa agaacctctt aaatttaata tgtggcgtgc
     5701 gcctacggac aatgacagga atataaagca taaatggatg gccgaaggct atgatagagc
     5761 aggtatgcgg gtatatgagt ccaaaataac aaacctgaca gataccttta ttgagattag
     5821 tgttgatttt tccctgggag ggtatataaa gctgccggta cttcatgggc aagcagtatg
     5881 ggtggttgat ggaacaggag aaatatatct aaaattaaat gcagaagtaa gagaaaatct
     5941 ggtatttctg ccacgcttcg gacttcaatt gactatgccc caaggaaatg aggaagttga
     6001 gtatttcggg tatggtcctc atgatagcta tatagacaaa aggcaaagtg tcaggaaagg
     6061 taaatatctt acaacagttg ataatatgtt tgaaaattac cttatgcctc aggagaatgg
     6121 ctcaaggttt ggcactgaat gggctattgt atcaaatgag cagggaatgg gattaaaatt
     6181 tacagggtgc cctgagtttt catttaatgc cgctcattat tcaccggaag atattactgc
     6241 tgccaggcac cctcacgagc tcaaaaagag gaaagaaact attataaatg ttgattacaa
     6301 gatgagcggg gtagggtcta attcctgtgg acctgagctt ctgggaaaat accgctttga
     6361 tgaaaaagaa tttgaattta gactaaagtt tatacctgtg tttaaagaag atgaataagt
     6421 actggtgaca attaagattg acttcaaccc agaaacttat ttagtatcaa taccttgaag
     6481 tttacaacac attattttac attctgagag cagatgctga tttggcgtcc tgtctcaaca
     6541 gctctttcta tgcacttttt gcctgtgagt tcgtagttct gctttttaaa gggggagaga
     6601 taggatgcct atatacatag tttattatca gcaaaatttt tatataaatt tttcatataa
     6661 tatgtatgga attacaaaag aaaagctgat cattaagtgg aaataatatt taattttgac
     6721 aacaatattc tatcccacca tctataatta tactctaaaa gtgaaaattt acgcataaaa
     6781 caacaatatt aatcatggat attaacccct ttcaatatta ttattcaact ataggataaa
     6841 aacgatattg ttccaacttt ctactgttta ggaggaaaaa tagatatggt accggaagga
     6901 ctaaaaatta cggttatagg gggaggtagc acatatacac cggaattagt taatggtttt
     6961 attaaatctg gaaatgaact tccaatttcg gagttttgcc tgatggatat agatgcacgt
     7021 aaacttaata tactcggggg cataactcga agaatgtttg cggaagcagg tattaattgc
     7081 accgttacct gtacggaaaa ccgggaaatc gctttgagaa attccgatta tgtagtatca
     7141 caaatccgtg tagggcagat ggatgcaaga atcaaggatg aaaagattcc tttggaatat
     7201 gacctgatag gacaagaaac tacgggtatc ggaggattta tgaaagctgt gcgcacaata
     7261 cctgttatgc ttaaaattgc cagggaaatg gaaaaatatt gcccggaagc atggttaatt
     7321 aatttttcaa atccatcggg gcttattgcg gaaactctct taaacaatac taatataaaa
     7381 tgtattggtt tatgcaatgt tcctctggga atgttaaaaa aggcatataa aattatgcaa
     7441 ggtgaacctg ataattataa agtagattat ataggcctga accacttggc ttggataaca
     7501 tcagtacatt ataaaaacag aaatatatta ggtgatttta ttgaaaagac ttgtattaaa
     7561 atgggcaaat actatgataa gattgatata cttcttaaaa atattaaggc agtcccttgt
     7621 ggttatcttt attattacta ctatagagat tattatctca aaaaacttaa agcacaggat
     7681 aaaactagag gagaagtctg tcttgagatt gaaaacgaat tattgcattt gtacaatcag
     7741 cccaatatca aacaaaaacc ggagctactg gacaaaagag gtggcggagg ttatgccgaa
     7801 gcactaattt ctttgttgag ttccatatac aataataaaa atgagattca tatagtaaat
     7861 gtgaagaatc aaggtgcact tgattttatg ggctatgatg atgtcgtaga ggtagcttgt
     7921 tatgtaagca ggaattctat cactccaata ccaatgaatg gttttaaaaa tgaacatata
     7981 aaagatatga tgagaactgt aaaagcttac gagaaacatg cagttaatgc cggcttatat
     8041 ggagattatt cggaagcgtt gaaggcgttg tttatacatc ctttggtcgg agattatgag
     8101 aaggcaaagg ctgctttgga tgctttgctg aaagcacata gggaatattt acctcaattt
     8161 aacatataaa tctattcttg tgaagaggaa ttttatacgg gagagagtat tttattgaag
     8221 aaatatgttt taggcataga tggtggctcc acaaaatctc aatatggctt gtttgatatt
     8281 gaaggtaata tgatcaactt tttacagggc ggatgtgtga atcatgctca ttttaccgac
     8341 aaatacgatg ggatggagag ggagctaaaa aaatgtataa ctgaaataac gaataaggca
     8401 gatatagata ttaacagcat tgaattctgc tttttaggaa tttcaggtgc agacagtaag
     8461 aatgatcata tagagatttc acgcaaagta tcgtgcatag gtattgggaa attcgaagtg
     8521 tgtaacgatg catttttggg tatcctggca ggcagtccaa aaaaatatgg agtttgcagt
     8581 gtaaatggaa caggaacaaa ctgtgcagct attgatagaa aaagacaaaa acttcttata
     8641 ggaggatggc cctatggggg tggtaattac cttatacact taatgctaaa gagagtatat
     8701 gatcattatt ttagatgtgg aaaatatacc attatggcag aaaaattgtt catggaacta
     8761 ggactatctt gcgaagatga aatggaggaa attgtataca aaaaggttta tgggagacag
     8821 attgatagaa gcagactaac ctccattcct ttttatgctg ttagcaaaaa tgatgaggtc
     8881 gcaaaagaga ttttggacat catggcattg gatgctgcaa gatcagttgc cggtgctgta
     8941 aatcgtctgg atttttcaca ggaaaaagaa gtggatgtaa ttctggcagg ttccatctat
     9001 gtaaaaggag aagatccata tctgataaat gcttttaaaa gatatacaaa agaactttta
     9061 aacgctgaaa ttaatttcat attacttgat gtgccacctg tagcaggtgc agtaattcag
     9121 gcagtggaaa ttgtaactgg attatgtgac aaagttatat atacaaaaat tataaaaact
     9181 ctaagtactt tatgtgaaga taataaataa taggttatat aattaaaatg tacatataaa
     9241 ctctatttca aaataacatt tgagggggaa taaagatgta ttgggaagaa ttaacgtcca
     9301 gggaatttga aaaggcacgt aaaacaacga agggtacatg cataatacct tttggtgtca
     9361 tagaaaaaca tggcacacat ttacctttga atacagatat gctggtaggg agaaaagtag
     9421 tttcagaagt agttaaaatt gagcctgctg tgatatttcc ctattattat ttcgggcaga
     9481 tagctgaagc catgcattgt ccaggcacaa tatccattga gccggagctt gcatatgcac
     9541 ttctaaaaaa tgtgtgcaaa gaaattgctc gaaatggttt taagaaaatt attattttga
     9601 actctcatgg tggaaatatc aggtttattg attattttat tcagactgca atttatgaaa
     9661 aaaaagatta cgtgatatat ggtgtatcag ccttgctaaa tccaaaagag cttatggaaa
     9721 tcagaacaat ttataattgt gataaggtag ggggtcatgg tggaaatcat gaaacttcaa
     9781 ttgttatgtt agaaagaccg gatcttgtgc acatggattg tatagaagaa gaaggactaa
     9841 aaaattatgg aaggataaac cacatctcta gagaaatggg gatacatact gctatgtggt
     9901 ggtatgctga tcatcctaca catttttccg gggttgttga aaattcttcc gctgaagcag
     9961 gtgagaaagc ttgcaggctt attacagaaa gggttgcaga gttagttcgg gctattaaag
    10021 aagatacaat agcaaaacag cttcaggatg aattttttgc taagagcgaa acattaggct
    10081 cacgagatta aatgagaggt ggtatgatga gtttatttaa gcatgacttc agcagattga
    10141 agaaggtgct tttaagagag ggtgaaccgg acagagttcc gttttttgaa ataattgttg
    10201 atgggaaggt tatagaatat cttacaggaa aagcacccag catttctgta aatgcagaat
    10261 tttacattaa acatggttat gattatgtta tgtcttcttt tggaattcct tacagaatgg
    10321 aaaaactacc tgctgctgat acatccttgg aactaaccaa agaacgaaga atgtttatca
    10381 atgaacaaaa aggcattatt ggaaacagat atgagttcga tacatataaa tggcctgttg
    10441 ttgatacaga aatactacat aaaataaaga agacgataga aatacttcca gataagatga
    10501 aaacagtaat ccttgcagga gatgggatac tggagagtgc aatggcactt atgggttata
    10561 cacagttatc atttatgata tatgatgatc ccctgcttat taataattta ttcgaaaagc
    10621 tgggtaaaga tctacttaaa gccttcgaat tatgtatgaa gaacgttaat gttaaaagca
    10681 taggtgctat agttataggc gatgatcttg gcttcaatac cagcactatt atatctcctg
    10741 atgatatcag aaaatatata ttcccatggc ataagaaaat tgttgagttg gcacacaggt
    10801 atgatttgcc tgtaatactt cattcatgcg gcaaacttga tgaaattatg gaagatatta
    10861 ttgatatagg gtataatgcc aaacattctt ttgaagacaa tattttgcca gtaacagaag
    10921 caaaaaagaa atatggagat agaatagcaa ttatgggtgg agtagatata aatgttttaa
    10981 gcactgcaac agaaaaggaa acaaggcagt atgtagagaa tattcttgat aaatgtatgc
    11041 ctggaggcgg ttatgcattg ggatccggaa acagcattac aaactatata tcggtaaaaa
    11101 actatctggc aatgttggat gaagggagga aaaaaggtgt gtattagtct atagatataa
    11161 atctttggca gttttaccaa tccataaaaa cttatatttt tggaggaata ataatgaatg
    11221 agttattgtt tggatacttt gataccgata taacaccatc tatttcaatg ggtctggaag
    11281 gttatcccta tcgcttaaat catggttttg aaaacggagg tgtaattgac cggatttatg
    11341 cctgtgcatt atgcctcaaa tcaggatcgg aagcattact aataattact gttgatcttt
    11401 gtgacttgag ctttgagact gctgatgata ttagaaatgc tataagcttg aaaacgggtt
    11461 tacccaggcg aaacattatg ctcattgcta ctcatacgca ttctgcacca gtgacaatat
    11521 gccagaatgt gaagaacaaa gaagccatag ataaaattga ggattattta aaagaactga
    11581 aaaacaagat tatatcaata tcttcacagg cttgtgccag aagatattct gcaaaactat
    11641 caatatcgac attaaagact tgtcttggat ataacaggcg ttatattagt gtggatgaaa
    11701 aaggcaattc aaattgtaaa atgcttttta acctctggca gaatcatgga catgatacaa
    11761 caggggaagt agactcggaa atccccataa ttatgattga aaaagaagat gatgaaaata
    11821 gggataattt tcttacaccc acaggcataa atagagttat tattgtaaat gctccttatc
    11881 atcctgttgt tatgggtgaa aacaacaact atattagtgc cgattatccc ggggcaatac
    11941 gaaaatgtat tgaaaatgaa ttgatggacg gtactaaagt tatttccata acgggggcta
    12001 gtggaaatac gcagcctttc atatcatgtg taaacaatct cagggcaata aggatatttg
    12061 gaaatgctat aggctatggc gttataacgg cccttgcagg aagaaaatac attccggttg
    12121 atgggattca aggaattgaa gaagaatttg actataataa tcctgaaact aatagaatta
    12181 gaacacaagt ctttagaata ggaaatattg gaatagcagg catttcgggt gactgtttta
    12241 atgaattggg tacattcata agggctaatt cgggtattag acaaattctt atagctactc
    12301 atgcaaatgg aagatctgga tatataccaa ctcctgaatc ttttaatatt ggggggtatg
    12361 agatagatat tgcaatcaag agaggttatg atacagatgt ttttgaaaag gtagtaaatc
    12421 aagtactcaa aaatttaaaa acattaagag aataataccg ctataaaggt agtatttaat
    12481 ttatgccgac gcacggcggt ggaatggtgg gacaaatatg agaaacggtg caataattga
    12541 aaaaggtcca atgaattggc aaataattca gcataaaaat ggaatagcag ttctttatct
    12601 ttcaggcaga tggattactg aaaagaacat gatacaacct cgtgtttatg caaaactctt
    12661 taatgaattt acaggagaag gcattacaca atggattgaa tgtgatacat ttgatgatgg
    12721 cacctggaaa gttagtctgg ataacattcc aaagggaggt ccttatagaa tagaaacatg
    12781 tctttacgat aaagcggata caaccatagg tattgaatgg gctttaagag gtgatatgat
    12841 attccatatt ggcgtagggg atctttttgt tatagcagga cagagtaatg ctgctggata
    12901 tggaaaagag cctgtttacg atcccccgga gataggaatt cacatttaca aaaataatgg
    12961 taaatgggat gtcgcaactc atccgttaaa tgattcaaca gatattatat atagtaacat
    13021 ggaaaattca aatccagggc attccccttt tataagtttt gctaaaacac ttaaaaatga
    13081 gcttggttac ccgataggtt tagttcaagc ttcacttgga gcaacatcaa ttagcaggtg
    13141 gaacccgggt gaggatggca ccttatatag gaatatgctt gagaaaatac aatctgttgg
    13201 aggaactgta aaaggcatac tctgggctca gggaggcact gatgctaaca ggggatatag
    13261 caaaaattat attgaaaggt ttaccaacat ggtaagatgt ttaagaaaag atttgaacga
    13321 caacttatta ccaatattca ccatgcagtc aggccgtcat gtaaaatttc aagataaaaa
    13381 taatgatagg gactgggcag aagttagaga ggctcaaaga caagcttcca aaaaactaga
    13441 caatgtctat actatttcat ctttgaatag catgttgtct gactatgtac atatgaattc
    13501 tttttctaac ataatgttgg gagaaagata tgcaaagatg gtacttacca agctttacgg
    13561 gaaaagcttt ctatgtagtg cccctgatat atcacgagca aagaaaacag atgaaaatca
    13621 agtaactcta tattttgaaa atgtggttgg gaaattatat gtttttgatc tagctgcaaa
    13681 cgaattacca tttacagttg aagatgagaa gggtaagtat agtatacttt catatgaaat
    13741 tttcgacaaa gataagatta ttttaaatgt tgatagaaca ttagagaaca atagtaaggt
    13801 tcattgtgcc tttgagcgta atccagggca atttgttcca atagaccgtg aaagctgtat
    13861 tcctatgctc gcattttacg gagtagagat tgaataatat gcatgtgcat atttcttgca
    13921 tcggaaacat tctgcagcaa ggaatctaca catagcagca gtgtgtaccc atagcttaga
    13981 aaaataaaat gcacgtagca gaaatggagc ttaaaatgaa tagtagagga cgagtattaa
    14041 ctgccttaaa acgtggcata cctgatgcag taccgtatat gtacaataca atggataagg
    14101 aattacagga aaaaataatc ggaaaagaaa tatcgctgcc tactgaaact ggaatgaatt
    14161 cactgggata tctaagcaga cccggtgatg aggcctatgt ttcacctata ttaacactag
    14221 tacctgaagt tgcagactta cttggtctag atgccatagg tattaaaata ttccctccac
    14281 tttttgtcga cggtggtata agggatggaa gggcaatgat aaaaaaggga cttttaaata
    14341 cccgtgaggc tttggagaag tgcaaaatgc ccgaccctga cgatgataag ttatataagt
    14401 cgattgaaaa gcttctggat gtatgtgccg gtgacagggc aatatatgcc aaaattaggc
    14461 ttggggcatc ttctactcta atgagtatgg acatgatggg attttcatat aatcttcatg
    14521 atgatcctga gcttgtttct gaagtattaa tgatgtattg cagttggagt caaaaaatat
    14581 gcaggaacct ctgcgaaatg gggttcgatt ttatctggtg ctttgacgat atagccttta
    14641 aaacagctcc tatgttttca ccagatgttt tgaataatat atttcttcct atattaatga
    14701 aaacaaccag tagtataaat aagccatggg ttttccatag tgatgggaat gttataccat
    14761 ttcttgatga tttgttgaag ttggatatgg atggactgca tccactggaa ccaggtgcaa
    14821 tggatctgga tagcttaaag tctaaatacg gttccaagct ttgcctcata ggcaatatag
    14881 atatagacaa aaccctgacc agaggcacaa tccaggatgt agataatgaa gtaagagaaa
    14941 gaatatgcct tttaggtaaa ggcggcgggt atatcatttc agattcaaac agcgtacctg
    15001 catattgcaa agcagaaaat gtaattgcta tgtctaatgc tgtaagtaaa tatagatata
    15061 tttactagat tgtttatagt taaataaaac gctgaaaaaa gcaaaaaagc tcatggctta
    15121 aatgctcaat aactaatata attaaaaatg caagctaaca aattaatatt tactgtttat
    15181 agcttagttg gcaatatgtc tagttagtaa taaatagttg gtaatatatt tcaggagagt
    15241 tggtttatat gaatgctgac ataaagaata aaaaaacaag aataaatata atggtaagag
    15301 atttcaagaa gaataagaca ttactgttat tagccttccc tatgatttta tatgttttta
    15361 tatttaagta tatacctatg tttggcataa taatagcatt taaaaatttt aaatataacc
    15421 ttggtttttt taaaagtccc tgggttggat ttaagaattt tcagtttttc tttacttcac
    15481 ctgatgcatg gctagttacc cgtaacacag tgggatataa tcttgtattt attactcttg
    15541 gtattattgt acaagttggt gttgcaatat tactgtttga gattgccagt agaaaacttc
    15601 ataaaatata tcaaaccttg atgtttatac ctcatcttct ttcatgggta gtagttgcat
    15661 atatggctta tgcttttcta agtccttcag gaggaatgct gaatcggtgg ctaagcttct
    15721 tcaacattga acctgttgat tggtatatta caccaaaacc atggatattt attttcccta
    15781 ttgcgaatat gtggcatggt ttgggaatgg gtgctcttat gtattatgca aagctaatgg
    15841 ggataaataa tgaatatttt gaggcagctg aaattgaagg tgcgagtaaa tggcaaataa
    15901 caaaaaatat aactcttcct tttttatatt ctttaattat aatgcttact atccttgcca
    15961 taggtcgtat ttttgatggt gatttcgggt tgttttatca gctgcccatg ggttctccca
    16021 tgctccgaaa aacaacagat atcatagaca cttatgttta cagagcactg gtagatgtag
    16081 gaaatgtagg aatagcaagt gcattgggac tttatagatc tttaatgggt atgattctag
    16141 ttattgttac aaatatgata gtcagaaaaa ttgatgaaga aaatgcttta ttttaacaaa
    16201 caattattag ccacagaaag gagctaattt aggatgagta tttctgcaat aaaacgcaac
    16261 acagtttcac aaataattat aaatatattt ttcattctcc tttcagtatc gtttataaca
    16321 ccattatttg cgattatatc aatatccctt actagtgagc gtgatgttgt aaattatggt
    16381 tattcactta ttcctccaaa tatagatttt tcagcgtaca agtttatttt atcaagtcct
    16441 tcaactatac tttcagcata taaagtgaca gctataactt catttatagg agtattatca
    16501 agtctcctca tatcatcaat gtgtgcattt tctctttcta gaaaggactt taagtataga
    16561 agacagttaa cattttattt cttttttact atgttattta atggaggact tgtgccatat
    16621 tatatattaa tgacaaagta tctttcactg agaaatacgt atcttgcgct tatacttcca
    16681 cttattggaa acatatggca tatgttgatc ttacgaacat tcttccagtc tcttatggtt
    16741 gaaattattg aatctgcaac tattgatgga gcaagtgatg ttttaatata tgcttatatc
    16801 gcactgcctc tatctacccc ggctcttgca acaataggac tgtttcaatt attgagtttt
    16861 tggaactctt ggtatcccgc acttttgttt atcaccaaaa aatcacttta tcctttacaa
    16921 tatctactac agtcaatgct tagaaatgca caggaaatta tccgaaacat ggaacaaatg
    16981 agctttatcg ctccagaaga attgaaaaat gttcctaccg aaagtaccag gatggctatg
    17041 tgtattatag caataggacc aatactgttt atattccctt ttttccaaaa gtattttgta
    17101 aaaggattga cgataggagc ggtcaaaggc tgagttgttt tttagagtgc atatcatgtg
    17161 ggtaattaaa tgctagtcat ttaatataac aaataaaaaa caggagggaa aatattatgt
    17221 caaagaagtt atcaaaagat ggctttctta tgggtaagta tgtaagaata ttatgcatcc
    17281 tgattgttgt aattatgttg tttgtgttgt cggtagggtg tgtaaaagtt aaaacagagg
    17341 gagaaaaaac agaatctaaa gtgacgactg aatcaactga gaccaaatct gaaccggaac
    17401 ctttaaaacc tgtcaaattg ttatggtatg taagagcaca aactccaaac aatgagaaaa
    17461 acgtattgga agctgcaaac aaaattattg gagaaaaaat taatgctaca ctggatttac
    17521 aatttattaa ttctggcgat tttaatgaaa agatgagaat gataatgagt gctcaggaag
    17581 aatatgacct tgcttataca gcaaactggg ctaacacatt ctcaaataac gtatctaatg
    17641 gtgcttatat tgaactggat gaccttttaa aagaaagaaa agctctgtat aatataattc
    17701 ctgaaaaaat atgggaagct ttaaaggtta atggtaaaac atatggagta ccaaactacc
    17761 aaataatgta taacgaaact ggattttggt ttaaaaagga tttgattgac aaatataaca
    17821 ttgaccttag taaggttaag aaaatggaag atcttactcc catttatcaa actataaaag
    17881 ataatgagcc tgatgtaata cctttgaggg ctggcaatgc aagcactgtg tttatgggtg
    17941 actatcatcc tgaaattgac aatctgttag cagttaatcc taaaacttgg gaagtttatg
    18001 atatgaaacc tacgctaatg gatcgttata agttaatgag agaatggtat caaaaaggct
    18061 ttttcccaag tgatgtagca actctgaaag atgaaaggcc attaatacta gaaggaaaaa
    18121 tatttagcag gattgtcaga ataaaaccag gtgtagaagg ggaactacaa gcatcatatg
    18181 gatatgattt tgttactata tccgcatcag ataaagttct taattattca ggtgcagctt
    18241 cagcccttac aggaattagc acaacatcaa agaatcctga gagagctatg atgctccttg
    18301 aacttaccaa cactgataaa gagcttctaa accttctaac atatggtatt gaaggtcagg
    18361 attatgtaaa aataggaact gacaaaatag agaaaaagcc cgattcatat acattctatg
    18421 catggcaatt gggtaatcaa tttattgctt atacattaaa gggacaagat gatagtgttt
    18481 gggaagagac taggaaatgt aatgaacagt caataattga tcctcttttt ggctttacat
    18541 ttgaccgtac acctgttgaa aatgaagttg cacaactgaa agctattaga acaaatttcg
    18601 atgatattat gaataatggt ttgactgatc cagaagaagc tatggctgac aggactcaac
    18661 aaatgaaggc tgccggtata gataaggtta ttgcagaaat gaagaaacag gtagatgcat
    18721 ggagacaaag taagtaaaca taaatactac caaaggcata gtttacaaga aaagtaccaa
    18781 taacaggtgc ttttcttgta aactacttta ttgcagtata gcttggtgta gtgatgattt
    18841 ttattgcagt taatcgctat attatatgcc tcgggcggaa tttatgcgtt tgttgttgca
    18901 aaaaatgcac atgcacaatt atatcatgca atttcttcga aattcatgat ataatagcaa
    18961 aagaatgatg gtaaaagggg aatagtatga aaagtataca gaagaaactc ctaatataca
    19021 tatcattagt atcagtattc cccattatta ttttgggaac ggtttcctat tatgtttctt
    19081 caaacgctct ggaaaagaaa ataaatgaga gttcaatgtc cagattgcat caaatagtaa
    19141 agaacctgga aaataagaat acaaaagtag agaatttttt aaatatgctt tctgcaagca
    19201 gtgaaattgg aaatatatta cggaactatg atcaaaacac aaaaccagtt gactattatt
    19261 tagctgcaaa taagttcaga caaacatttt tatctttttt ttataatgat aagagtattg
    19321 attcaggatt tattctttca aataaaggtt ttacctacga atataactgc tatattgaaa
    19381 aaaacaaatc tttaaaacta ttagattggt atgataaaac aactgaaaat aaagtagata
    19441 acacatgggt aagttatatt gaaaagccca atgatattat taaatcattt acaaaagccg
    19501 atgatattat actcgtaggg actaatgtaa gggatattaa ctatgcttcc gacttatcgg
    19561 tattgggaat tgcatgtata ttcctaaaca agtcgttttt ctcaagtatt tatgagggag
    19621 tcgatgaact gcctgataca aacacagtca taattgatga taaaggaaat atagttataa
    19681 caagtgataa ggctgcattt tattctcaac ttgataatat atcagggtat tcttttgcaa
    19741 aaacagtgtt ggaaaataat aaagggttat ttaggtgtca ggtaaataaa tcagatttag
    19801 ttgtaagtta tatgacacta aatacaaatg gatggaaggt attgcaagtt actccttaca
    19861 gttattatat acgtgaaata acctccatag ggtggatatc catattactg gctgctattt
    19921 gcctttgtat aatatattta ctggcatttt tcgtttcaaa atccatatca cgtcctcttt
    19981 tgaatattgt taaaatgatg gataaagtaa caaatgggga tttccaagtg actgtgccgg
    20041 taactaggaa tgatgaaata gggattattt gtgcagggtt taacaatatg gttaagtcaa
    20101 taggagaact tttctctgct ttaaaggaag aagaaaggga aaaaaggaag gctgagatca
    20161 tgaaactgca ataccagatt aacccccact ttttatataa tactttagct tctatacgtt
    20221 taatggcatc gcttataaaa gctgatgaga ttgcaaggat tatcaaaata ttaagtagat
    20281 tgttaagaaa tacaataagt aaagctgacc aacttacaac tatctatgag gaatttgcga
    20341 atgctatgga ctatatcgaa attcagcaaa ttagatatga caataagata tttgtagaaa
    20401 aggatataga aaaagaaata ctacctttaa aaataaaagg gatgctgtta cagcctatac
    20461 tggagaattc aataatgcat ggattagctg aaaagctgaa taacggcagt aaagctgctt
    20521 atttgaaaat atcggggtac aagaaacaaa acaatattta ttttgaggtt tttgacaatg
    20581 gaaagggtat gactcctgaa caaattaaga atgtatttga aaagaatgat tcattagaag
    20641 gtcagattgg agttaaaaac attcataata gaataaggct tctttatggg gatgaatatg
    20701 gagcaagcat agaaagcgtt cgaggtgaat ggaccaaggt tgttttaagc ttaccttatg
    20761 aaaatgagga ggtagataaa tatgtttagt atattaatag tagacgatga aaccttaata
    20821 aggaaggctt taaaaacatt aattaactgg gaacagttag gttttaagct tattgctgat
    20881 gtatataatg gaattgaagc tttaaagatt attgattcct gccatgtgga tattgtcctt
    20941 acagacttga aaatgcctga aatggatgga cttgagctaa tgaggaacgt aataaaaaaa
    21001 tatcctcata taaagtttat tgctttaagt gcttatgatg aattccacat ggtaagtgaa
    21061 gcgtttaaac tgggtgcaaa ggaatatttt ctaaaaagcg aaatggaaga agatgatatt
    21121 gttaaggcaa ttcaaaacat taaaacagaa atagagagag ataagctgga ggaattacat
    21181 aaaaagaaaa tacgggatga aatgaaatcc agtggaaatg cattaaaaca ggaaatattg
    21241 cttgatattg ttatgaggaa caggatagcc acagacagag agctgaagtt acttaatgat
    21301 agatatcttc ctgctttcaa tccagaaaat atgcgagtga tgcatataaa tataggtaag
    21361 tatttaaaat tagaagataa aattgaagta tacaattatg agaatataaa aaataatata
    21421 tataatgcta taaaacaaaa aatagaagag tacacaaacg tggaaatcct ttatagatac
    21481 cctgatgaaa ttattctgat ctgttctttt tctaatttag ttagtaagcg gcagttgatt
    21541 aatgaatttc ttggtttttt ttcagatata aataactata tatataaaaa atacaatttg
    21601 aaaataactg ctgggttgag tgggtacggt agagggtata attccttgaa tagcctgtat
    21661 atacaagctt taacagcgag tgaacacagt tttatatttg gaaagggtaa acttttaatt
    21721 tattcaaatc tacctataaa taataagtct gttaatatag aaaaacacca aaagcctatg
    21781 gcttttagag atcttttaaa aacaatggat gcagatagaa tatccagagg gtatgtaatg
    21841 ttcgtgcaaa agcctgaaga tgcaagttgt aagaatatta tagatattaa aataatgttc
    21901 cagcagtatt actttatagt aatggaattt acccaccatg ttaatataat tgataatata
    21961 aatgaattgg cggaggaata tgaaaagtat ttaagggata tgggagattt gcttgaattg
    22021 aataactggt tgaaacggtt aatgcaaaag attgctgaaa cattagaaaa tacaagcaac
    22081 ctgatattga gtttaaagaa atatatacat caaaattatt cacagaatat agtcttatca
    22141 gatgttgcag aaaaatatag tataagcacc tgttatctca gtcgggtatt tgcagaagaa
    22201 gtaggctgta attttgcaca gtatctttca aaggtacgta tagaagctgc tattgattta
    22261 ttaataaaca ctaatttaaa gatatatgaa atagctgaac gtgttggtta ttccaattct
    22321 gagcatttca gcagaagttt caaaaaaata attggtaaaa gtccaaagac ttattcaaga
    22381 taaggcatga aaggtataga aagttatgat atgacaatat ggaacaaata taatctacag
    22441 tcatgctata taatttacga tttgaaaaag ctaatgaaga attacaaatc gtaagttata
    22501 tcaaggtaac agaagcaggt tttaaatgtt ttgaaaacct gttctatata aggaggaata
    22561 attttgaaca ggaatgtatt ttcacggtat atggctttat ttgcaactac aatagcgatt
    22621 ttaattttct attcatcctt taagcttatt gaaaagggta aagctcatag ctcatatata
    22681 atacaaaaca atgaagaaat aacacaaaat aacaataata aaaacatagg tattgaggaa
    22741 aagcaaagag aatataaaat atcatgggct tcgtggacac caataaaatt taaggagaat
    22801 aatagttggg gtgagaaatg catagaagaa tactttcctg atgttgatat tcaattcatt
    22861 cattttgata aaagtacttg ggaaatccag ttgagtgctt taattgcaag tggcacatat
    22921 cctgatgcta taaggttaaa ggataggact atgatatata catatgtaag acaaggagtt
    22981 ttggcaaaag caccttattc tgtagtaaag gaaaatgctg gaaggtatat tgaagctatg
    23041 gaagaaacag gagttgatca ttggcttaca actttaataa atggtactaa ttacggtata
    23101 cctgtcttaa atgtgagaag tggttcggat tattgcaatg tgtggagaag tgactggcta
    23161 gaaagcacat caatatataa aataccagat acattagaag aatatgagga agcttttagg
    23221 aaattcaccc tggaagatcc ggacgaaaat ggtatagatg acacttttgc atatgctcca
    23281 ataggtacgg aaaaagatat ccaatatcag ttcaatacta tatttgctgc acatggagtt
    23341 ttcccattca catggatggt aatggaagat ggcacttata aatatggttt ggtaatggaa
    23401 gaggcaaggg aggccatgag gagattgaac tcatggtata agaatggtta tattgatccg
    23461 gattttatga aaaatgatgc tgaaatagct aaggctaagc tgcttaatgg taaaagtggt
    23521 tttagagaat ggaggggtat atgggaatgg agacccccaa gagatggtgg caatgcagga
    23581 tatgaatatg cagaagcatt aaaacttaat cctaattgtg agtttaaaat gggataccca
    23641 ccagaaggtt ctagtggaaa aagggggctg tatgattggg gtaatgttac cggagcagtt
    23701 tgttttggaa agcatctaga taatgaactc caaaaaatgg gcaaaataat acaaatttta
    23761 gaattatttg ccacaaatga agaagctgct ataaagctgg aatatggaat agagggagaa
    23821 cattacatat atgattcagt aacgggaagc aagaacagac tacctccata cgacagtgga
    23881 aatgaagggt ggactttatt aagtcccaat ggactattcg gttggtcacg tataagagaa
    23941 gtatcagatg ctttacaaga taattatatt gtagaggatt ttggttggaa ttcaaaaaat
    24001 acgttgttag caggtatcaa ttatattcct tatgtaaatc aatttgttga tttggagatg
    24061 ttaaagtatc agatacaagc aagggaaata gctgatatat gggaactcga atttattacc
    24121 gggagtaaga gtctagatga tgattggtat gaatttataa aagcctggaa agagtctggt
    24181 ggtgagattg ttacgcagca ggttaatatg atggctagga aagcgataga agaaaatctg
    24241 cagaatatag aaagcagcat taatgcgata acgaaataag acttagatca attagcataa
    24301 tcagggtgga aaaattaatg ataaataaag cagaaaatga aaatatataa cattatgatt
    24361 ttgagttggc ggaaaatggc tactatgggc ttctaagtta ttaaggacaa aagaatacag
    24421 aaaatattaa cttacatcat gtttgttatt aaatgatgtt gctacactta gcatatctat
    24481 ttatcttata ttatttagta caaaagaacg cataaaatat caacttatat catgtttatt
    24541 ataaaaagat ggtgctacac ttaacatatc taattgtctc atatttataa gagaaatatc
    24601 gctattattt taaaactctt taattaa
//
LOCUS       DAOSDD010000032        24621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_905_length_24621_cov_5.207116, whole genome shotgun sequence.
ACCESSION   DAOSDD010000032 DAOSDD010000000
VERSION     DAOSDD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_24621_cov_5.207116"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK733_04690"
     CDS             complement(<1..362)
                     /locus_tag="PK733_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOJ09873.1"
                     /translation="MNMNKSFIIKKRFNGLGLYIIAAAVLTVLIFSMFYSNSVSQGLA
                     ENLIRLHVVADSDSVEDQALKLEVRDAIIEYMGVKFNETKDIIQTKSIINSNLNNIID
                     IAQRTIKSHGKEYPVKASL"
     gene            complement(460..2073)
                     /locus_tag="PK733_04695"
     CDS             complement(460..2073)
                     /locus_tag="PK733_04695"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOJ09874.1"
                     /translation="MAVKYIFVTGGVVSGLGKGITAASLGRLLKARGLHVTIQKFDPY
                     INVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLNKNSNITTGKIYLSVISK
                     ERKGEFLGGTVQVIPHITNEIKDRIYRVARANSTDVVITEIGGTVGDIESLPFLEAIR
                     QFAINEGRENVMYIHVTLVPYLGKSGELKTKPTQHSVKELRSIGIQPDVIVCRTEKYL
                     TKDMKDKIALFCNLPEDGVIQNMDAEVLYDVPLMLEKEGLGNIVCKRLNLQCKEPDLT
                     EWEEMVLKQKNQTKKVNIALVGKYVELHDAYLSVVEALKHGGIANDAEVIVDWVNSEE
                     LCDENVDDILGQADGILVPGGFGDRGIDGKIRAAKYARENKVPYLGICLGMQMAVVEY
                     ARNVCGMKDAHSSEFNFNTKYPVIDLMPEQKDIDEKGGTMRLGLYPCKLKSGSKVHDI
                     FCEDLIYERHRHRFEFNNEFKSRLTEKGMILSGLSPSERLVEIIELPDHPWFIGVQFH
                     PEFRSRPNRPHPLFRDFIKVAYDRRNTKI"
     gene            complement(2279..3805)
                     /locus_tag="PK733_04700"
     CDS             complement(2279..3805)
                     /locus_tag="PK733_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ09875.1"
                     /translation="MDKERFYIRNPYLEIKRIIIMIIIICLILTYPASMKYVLASENE
                     GFPSDYLNSVVEMIKEKYIGDVDSEQLLEGALKGMFGTMDPYTGFFNRDEAERFFGDV
                     DGSYEGIGVSMEKIADTIVISKVFSGSPAEKAGMLQGDKIVAVNGKSIIGASTEEVAA
                     LIKGEAGTKVTLGIIRDKSVEIINIDVGREKIKINPITYEIKDDIGYIKLEMFNANTE
                     EYLNQALKEIDDMGINKIILDLRDNPGGLVDQGVALAGKFVPKGLITKLIFKSEQTKD
                     QEYYSYLPKSKYRLAVLVNSMSASASEIVAGAIQDTKAGVIVGTKTFGKAKVQSMLPV
                     LTPEAYKKYSERTGDKFIGAYELITKYNINLLTDEILGWTKITTGVYVTPDGRMIDGK
                     GITPDVQVEDTPTVEGVYLKNIHKLTITWKPALGDEGIDVYNAEKILRILGYDVDTPD
                     NRLDKKTYDAISKFRTDKGLYPGGVLDFTTQKALNTELERIYNTSDKQYSKAIEILKQ
                     "
     gene            complement(3991..4752)
                     /gene="sleB"
                     /locus_tag="PK733_04705"
     CDS             complement(3991..4752)
                     /gene="sleB"
                     /locus_tag="PK733_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HOJ09876.1"
                     /translation="MRKLSKYRKHIIPAVLILAIVTGVTFYYKNYLYNLYYTISRNIS
                     GSISGSSSGDIPGKSRETLSYYGSSGQEVINIQTKLKQWGYYKGDIDGIYGSLTYQAV
                     RDFQSKNGLAVDGIAGSQTLAALGLPTGEAPAYSSNKDTNILARIIHGEARGEPYTGK
                     VAVGAVILNRVRDSRFPSTIAGVIYQPGAFDAVADGQINIEPDSSSYKAAQDALNGWD
                     PTYGCIYYWNPATATSSWIWSRPIITRIGKHVFAK"
     gene            complement(4934..6628)
                     /gene="argS"
                     /locus_tag="PK733_04710"
     CDS             complement(4934..6628)
                     /gene="argS"
                     /locus_tag="PK733_04710"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ09877.1"
                     /translation="MINVMENMRQIVRNTVEQAILKAVAKGDIPEVDFPEVLIEIPRE
                     KNHGDFSTNIAMQITKLLRMPPAKAASIIIENMELNGTYIEKVEKAGPGFINFYLNNQ
                     WLYYTLKVIQKEKNNYGRNDIGKGQKVMVEFVSANPTGPLHMGNARGGALGDCIASVL
                     EAAGYDVTREFYINDAGNQIEKFGISLEARYIQLLNGEDAIEFPEDGYHGEDITEHAR
                     NYIENYGSGLLEAECAERRGKLIEYALNKNIENIKKVLENYGIKYDVWFPEKSLYESG
                     ELDETLNILKQKGLTEERDGAVWLKVSQLGAEKDEVLVRNNGFPTYFASDIAYHRSKF
                     LKRGFDRVINLWGADHHGHVGRMKASVKAFGIDSDKLDVVIFQLVRLYRNGEIARMSK
                     RTGKAISLADLLEEVGKDAARFFFNMKASGSHLDFDLDLAVSQSNENPVYYVQYAYAR
                     ICSMLRLLESEGVNVPDVDQVDLALLKEQEEIDLIKKLVEYPEEIKVSAQTLEPSRLT
                     RYVIDTASSFHSFYNACRVKGEEDELMKARLVLVECVRIVIKNVLDLLKINTPERM"
     gene            complement(6591..7058)
                     /locus_tag="PK733_04715"
     CDS             complement(6591..7058)
                     /locus_tag="PK733_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HOJ09878.1"
                     /translation="MDKNVIISVKGNQNSEQGISDSMELVTEGRYYKEDNIYYVTYDE
                     SEVTGMEGTTTTIKVADDVVTLERAGAVNSQFIFQKGQKHISYYDTTHGAFTIGVFAN
                     SVNVKIDDSGGEISIDYQIDIDNNKMGYNDFCMSIREAGCSNDKCYGKHETNS"
     gene            complement(7100..7912)
                     /gene="murI"
                     /locus_tag="PK733_04720"
     CDS             complement(7100..7912)
                     /gene="murI"
                     /locus_tag="PK733_04720"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOJ09879.1"
                     /translation="MDNRSIGVFDSGLGGLTVLKEIMNLVPGESIVYFGDNGRVPYGT
                     KSKETVIKYTLQGMRFLLNQDIKMIVIACNTASALSLDMVKSSFHIPVIEVIEPGAAT
                     GVKYTKNKRIGVIGTTATINSGAYEKAINRLDNSIKVFSKACPLFVPLAEEGPEWWEN
                     DITLQIAAEYLSALKQKEIDTLVLGCTHYPLLQKVIQRVVGNEVSLVSSALEVARVVK
                     SIIEKTCIERDGKIKPVYRYYTSDSVEKFEPLCSAILSMEINSAERIDIEKY"
     gene            complement(7924..9063)
                     /locus_tag="PK733_04725"
     CDS             complement(7924..9063)
                     /locus_tag="PK733_04725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOJ09880.1"
                     /translation="MNHKKRVAVIFGGQSTEHEVSRFSAQSVIENLDKSKYDIVMIGI
                     TKEGKWLTYSGPAEKIGTGEWQAIAESQRLRPEKIELGSVSGSVSGGTVDYSNSARNI
                     LIASGAENDIKKVDVVFPVLHGLNGEDGTIQGLFELAGMPYVGCEVLGSALCMDKAFA
                     KIVFDKEGIPQGKYIAFNKRQFQIDTDKIISEVENILEYPCFVKPCNSGSSVGVSKVR
                     NRRELIDAISLAGMYDRRILVEEYINGREIECSVLGNDNPVASTVGEVIPDKEFYDYE
                     AKYSSESTSEIIIPARLPESSIEKIREYAVKAFKALDCAGLARVDFFVHKETGEIYIN
                     EINTMPGFTKISMYPKLWEASGLPYGELLNKLIELSIERYNEKTE"
     gene            9419..9637
                     /locus_tag="PK733_04730"
     CDS             9419..9637
                     /locus_tag="PK733_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ09881.1"
                     /translation="MPFYDLKCKKCGEEFNKMAKISEREQNLITCPKCGSNKLDPIFT
                     NVNYVVSSKKEGPSCPNIDRCGGCCGLN"
     gene            complement(9697..10404)
                     /locus_tag="PK733_04735"
     CDS             complement(9697..10404)
                     /locus_tag="PK733_04735"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ09882.1"
                     /translation="MRKVFYVKIISITTMLVLVVALISGCSVLQKLGFQKNDIDEIHP
                     VSFVYMDEIEAARISEKIPVRLYFTNEDNSKLMTEIRYIKLEGDGKNPGDLASEIVRQ
                     LIKGPGEGTGLKATIPEGTELRSPVTVEGKVATVDLTKEFIDNHPGGKDAEKMTIYSI
                     VNSLTQVKNIEKIKFTIEGKTRDKFKGNFKFDAPFPGSPSLNSKEPPVKSKKTADEAL
                     DGEDKETSGEIEEEILE"
     gene            complement(10571..11113)
                     /locus_tag="PK733_04740"
     CDS             complement(10571..11113)
                     /locus_tag="PK733_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HOJ09883.1"
                     /translation="MILGNALEFDENTVFRSIRKDNMTISDVYDEILNFIRSDTECSY
                     KISIGTDSQSGTGTVFVSCILVHRVGRGAIGFLHKAQIPRPIKSLREKIYLETCASLQ
                     LAYLFDDDKIQNIINTVKSGDKSRGVSFEFHIDIGTKGPTKALISEMTGMVKGLSFVP
                     KIKPDSYCASSFADKYTKAM"
     gene            complement(11411..12292)
                     /locus_tag="PK733_04745"
     CDS             complement(11411..12292)
                     /locus_tag="PK733_04745"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOJ09884.1"
                     /translation="MHLGFRQKVKDFILTMRPKQWIKNAFVFAGLIFSKSFMNLALIL
                     RSIYAFILFSLVSGIVYIINDIIDRDKDKLHPLKAKRPIASGRLKPDEAIIPALVILC
                     AVIFASYKLDFLFFIIIFTYFLLILFYSIILKNIVILDVVIISVGFVLRTLAGTAIIR
                     VEISPWLIMCTTFLALFLALNKRKNELILLSEDAANHRKNLEQYTPELIDQMLTVVSS
                     ATIMSYSLYTFTSGKSHYMMLTIPFVLYGIFRYQYLVSSGNDGGSPEIVLLKDLPLII
                     NIILWGITSIIIVFFFY"
     gene            complement(12340..13965)
                     /locus_tag="PK733_04750"
     CDS             complement(12340..13965)
                     /locus_tag="PK733_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09885.1"
                     /translation="MSGNFKRKDSKNLCSKSHDDKTNGIMGKGNGIMGKTDEIRGKNY
                     KVSVYATLPFLIFLSVCGFLIISYSISKYGPGISHDSIAYIYAAESFLSGRGLIYFAY
                     DTPLIQWPPLFPLILAGLKSTGADVIVAAGFFNSLVFTLIILISGLWLLKCTEDGIIA
                     VAGSFAMLASIPLYYVSRFIWSEPLFILLTLLFLINLDCYIRTSSPKYMAGASICTAL
                     ACLTRYVGITLIISGIIILLFQKKKILNRFIEIVIYGFISALPTSIWVARNYMLTGTL
                     TGGRPPSENTISENAGTLFKIIAAWFVPAGSENMAYSILGIFIFIASIIAIRNHSDQK
                     SNRLAILLIFPVIYIAYLLLSASIVAFDSINNRLLVPTYIPLVLLQFFSIAYIVKTDK
                     GKKNKIITVLVFVIIFISWIIYPISEISTDAAYAYENGAGILASKWWAQSPLIAYVKE
                     LPQNSAIYTNCPDAVYFYTGRKSMYTPKKEGPDIYGLDEFKKIVYEAENVYVAWFDVG
                     TGNFIYNAEELANFFNVEMIKKVYDGIIYKIEK"
     gene            complement(14051..14923)
                     /locus_tag="PK733_04755"
     CDS             complement(14051..14923)
                     /locus_tag="PK733_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ09886.1"
                     /translation="MLKYDVIIIGKGPAGLSAALYTARANLKTLILGKNDSALGKAAK
                     IENYFGFSSPVSGKFLLDEGEKQALNVGADIVNNEVVSIEKSDAFEVTTSEEVYEGIA
                     VLIATGQPQKKLKIKNMEKFEGNGVSYCTTCDGYFYNNLKVGVLGNKDYAVHEAMELE
                     AYTKDITIYTNGKELDLTEKYSGEIAKYQINTKSITEINGADYLQRICFADETWEDLD
                     GLFIANESASSIDFARKLGVITEGNSIVVDSEQRTNLEGLFAAGDCTGGFKQISTAVG
                     QGALAGRKIIEYIR"
     gene            complement(14939..15652)
                     /locus_tag="PK733_04760"
     CDS             complement(14939..15652)
                     /locus_tag="PK733_04760"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOJ09887.1"
                     /translation="MSAKGVNWGKLKNVLYWIVMSVLIAVILYNLYNRYAGKTPIKMA
                     PGQTKKIENSEGTDNTKGTDTTSGTEETEKADKTNGKEQVEEGQKDGTDQDSKQQEEK
                     IMAPDFELVDLEGKKVKLTDYRGKVVFLNFWALWCPFCVQEMPDLNSANKKFLEGEDA
                     VIITVNSGDSADKVKEFMKEKKLSLPVLLDTDGSISNAYGVSGIPITFIIDSEGAFYA
                     YISGATNEKTLMEVVEEMK"
     gene            complement(15690..16364)
                     /locus_tag="PK733_04765"
     CDS             complement(15690..16364)
                     /locus_tag="PK733_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOJ09888.1"
                     /translation="MGGSSYIIGFIEGILTFISPCILPILPVYFFYLAGVSAENNAVK
                     NRYRLLFNSLGFVLGFSIVFITLGAAATTIGHFLNDNIGLFRKISGILMIIFGLNFTG
                     IIKIDFLNTEKKFDYKFKELKFLSSIVFGMIFGFGWTPCAGAFLGSVLLMASNSGTVK
                     EGVFLLSIYSLGLGVPFILSSLLFSEIKDSFRWLQEKGRIISIVSGVILIAAGILVYA
                     DLLKYV"
     gene            16953..17198
                     /locus_tag="PK733_04770"
     CDS             16953..17198
                     /locus_tag="PK733_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09889.1"
                     /translation="MDILQNSLTNLSRFFSEGGDELLIPLIILLTQFKDDKEERSSGL
                     KSFDFNSILFFGALFFILFISRYFEKEMTRVNLEEIS"
     gene            17277..17597
                     /locus_tag="PK733_04775"
     CDS             17277..17597
                     /locus_tag="PK733_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane trafficking protein"
                     /protein_id="HOJ09890.1"
                     /translation="MGKDFNKIFAEMLGKMDEKVLQAKLNAALDMLKKGKTDDLAKKI
                     NKIDKNELMEKINEFDEEKIKDMNINLDEIKSKVTENDLENLKSLVGEHGDEIIAKIK
                     DIIK"
     gene            17656..18048
                     /locus_tag="PK733_04780"
     CDS             17656..18048
                     /locus_tag="PK733_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09891.1"
                     /translation="MDDDLGKTIKQITDMLSKDKDKMPDNLMGLISLLTSPGSSESGS
                     ENDVEREEKNEKEGNNVNRSNKGDLQDNLEMIKQVTKIMDRLNTKNDPRVNLLNALRP
                     FLNNKRQKKINNCIMLLQVTGIMKAMDE"
     gene            18063..18467
                     /locus_tag="PK733_04785"
     CDS             18063..18467
                     /locus_tag="PK733_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09892.1"
                     /translation="MAYRRYPYIHRNYYYTRNLPNGKNNAPTKLVNDIPMPNEKESIR
                     DPEVPLKPGSKTSSKASSESSFKIGHDSSNNNIPDTPDNKKRDYKSPFIKLFQDKIQL
                     DDIIILGLIFILLYEGIKDDMLFLILIYILLG"
     gene            complement(18481..19332)
                     /locus_tag="PK733_04790"
     CDS             complement(18481..19332)
                     /locus_tag="PK733_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09893.1"
                     /translation="MESIGNAIGVILKRPFIIIFWAILMLICTVAGYILPVFRTLFQL
                     NTISGSNPFETIMSFIQLVYSYMLDIKVVIIAAVVLLLVLLSIAVISGALISGYFFKL
                     NNAVNKKAGKKGELSHGVRQYFNKITLMNFVLLATGLIILIIIVVASVPALVITRASA
                     GKGRDMLPAVLFVDVLTGVVLFFGLMFYRIYTFFWYPAAMNNEKRPFRQSKRIANNNF
                     WRIVSVFVVFDIVYIISQIFFVKLDDNIILLLAKWAFSSVFYAVYTTYIFAAYRKYSD
                     NNPESLQ"
     gene            complement(19433..20650)
                     /locus_tag="PK733_04795"
     CDS             complement(19433..20650)
                     /locus_tag="PK733_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase"
                     /protein_id="HOJ09894.1"
                     /translation="MKKAVMYGAGNIGRGFIGQLFSQSGYEVVFIDINPIVIEKLNSD
                     GAYPIRVVAHDTYNEIVIRNVRGVNGTDIESVAREISEADIMATAVGVNVLPKVAGPI
                     SMGLKKRWQGKNMDPLNIIICENMLDANRYLEKLIKQELNDEENKLFDEKVGLVEASI
                     GRMVPVMTEEMQEGNPLRVYVEEYCELPVEKDAFKGEIPSIINMVPFSPFDLYIQRKL
                     FMHNMSHATAAYLGFLNNYTYIWETCKNPAIKLITMKALLESSIALSLEHNYPLQKLV
                     EHAEDLLYRFGNRLLGDTVARVGRDTVRKLSKNDRLVGAAELCVKQGIVPAYICIGIA
                     AGYIFAPEDDRFASQVRQTVASEGIEKALGQYSCTDSESPLFNIVVKFYNMIKDGASL
                     EQLEQTAEQLKKK"
     gene            complement(21285..21455)
                     /locus_tag="PK733_04800"
     CDS             complement(21285..21455)
                     /locus_tag="PK733_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09895.1"
                     /translation="MRKEIKRRKQKNTNDGPVFAPGLSNDALEKRASVRDILKGNYTK
                     VTKVYLDENDPS"
     gene            21882..22841
                     /locus_tag="PK733_04805"
     CDS             21882..22841
                     /locus_tag="PK733_04805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ09896.1"
                     /translation="MKTVIVGDNQGNRKISKFIKDNFGSMPVSAMYKAFRKKDIKVNG
                     KRVSEDYIVMPGDKLDIYIPDNILNGTPIGNSGLLSRNSHDCSTRGFSVVYDDSNILI
                     VNKDQGIPVHPDVNQEEDTLIDSVKCYLQWKGEYDPLDPSSFSPTLCHRLDRNTGGLV
                     IIAKSSISLSIILEKIKNREIKKYYQCLVKGKMEKECDELRAFLEKDERNSRVFINNT
                     RKPGSLEIITKYRVLSYENDMSKLEVELVTGRTHQIRAHLAFIGHPVVGDGKYGINSY
                     NRALGVKKQALWAYKILFSMKKDSGILNYLKGKVFEIEPQFTI"
     gene            complement(23164..23970)
                     /locus_tag="PK733_04810"
     CDS             complement(23164..23970)
                     /locus_tag="PK733_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOJ09897.1"
                     /translation="MKKRIILVMVLALIFTSAFSFFADNITSVKAEPAISVLGYLDAT
                     VTANFLNVRQGPSTNHKVICVLKKGQSVKVFAKVGDWYALQESGMGCIGVAHGKYLDI
                     AWPKAEPKPTPQPTPQPPQPEPKPEPKPEPTPEVAPPSDATADEKALLDLVNKARADA
                     GAGPLKFDSELLKVARLKAKDMVDKNYFNHDSPTYGSPFDMMRQFNISFRTAGENIAG
                     NRTVDGAFKAWMNSEGHRKNILNGSFNYTGIGIVESSKYGKILVQMFIGR"
     gene            complement(24171..>24621)
                     /locus_tag="PK733_04815"
     CDS             complement(24171..>24621)
                     /locus_tag="PK733_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ09898.1"
                     /translation="STALNCVYLDENGKEQVMIMGSYGIGVGRTMAAIIEQNYDEHGI
                     IWPASVAPYHAIVVPVNQSDKVQAKLAEQIYNMLADNGIEVLIDDRDERPGVKFKDAD
                     LIGIPVRITVGRKAEDGIVEYKLRKSSEIKEIKVENILNEVKKAVGN"
BASE COUNT         7542 a         4993 c         3695 g         8391 t
ORIGIN      
        1 agagatgctt ttacagggta ttcctttcca tgggatttta tagtcctctg agctatatca
       61 attatattat ttaaattact gttaattatt gattttgtct gtattatgtc tttggtctca
      121 ttaaatttta ctcccatata ttcaatgatt gcatccctta cttccagctt taaagcctga
      181 tcttccacag aatcactatc cgcaactaca tggagtctga taaggttttc agccaacccc
      241 tgggaaacac tgttagagta aaacatcgaa aatataagga cagttaaaac agcagctgca
      301 attatataca aacctagtcc attaaatctt ttttttatta taaaagattt attcatattc
      361 ataaaaaatc cccctgcatt tcatcttatt tcattaataa gtattgccag ggggaaaatt
      421 ttttatactg taattctaat tattatgaaa accgccaatt taaatcttcg tatttcttct
      481 gtcatatgca actttaataa aatctctgaa taaggggtga ggcctgttgg gtctcgacct
      541 gaattccgga tggaattgca ctcctataaa ccagggatgg tcgggcaatt cgattatttc
      601 aaccaatctc tcactaggtg acaaacctga taggatcata cctttttcag tcagcctgga
      661 cttaaattca ttgttaaatt caaatctatg cctgtgtctt tcataaatca ggtcttcaca
      721 aaaaatatca tgtaccttgc tcccggattt taatttgcag ggatacaatc ccaatctcat
      781 tgtaccacct ttttcatcaa tatctttttg ttccggcata aggtcaataa ccggatattt
      841 tgtgttgaaa ttaaactccg agctatgtgc atctttcatc ccgcatacat ttcttgcata
      901 ctctacaact gccatttgca ttcccaggca tattccaaga tatggtacct tattttctct
      961 ggcatatttt gcagctctta ttttaccgtc gattcctcta tctccaaagc cacctggtac
     1021 aagtattcca tcagcttgtc ccaaaatatc atccacattc tcatcacaga gttcctcaga
     1081 attaacccaa tctacaatta cctcagcatc atttgcaatt ccaccatgct ttaaagcttc
     1141 gacaacactt aaatatgcat cgtgtaattc cacatacttg cctaccaatg caatattcac
     1201 ctttttagtc tggttttttt gttttaaaac catttcttcc cattctgtca gatcaggttc
     1261 tttgcattga agatttaagc gtttgcatac aatgtttcct aatccctctt tttcaagcat
     1321 aaggggcaca tcatacaata cttcggcatc catattttgt attactccgt cttccggaag
     1381 attacagaaa agagctattt tatccttcat atcctttgtc aaatatttct ctgtccgaca
     1441 tacaattaca tcaggttgaa ttcctatact cctcaattcc ttaacgctat gttgtgtagg
     1501 tttagtcttt aactcaccag acttgccaag atatggaaca agagttacgt gaatatacat
     1561 aacattctct cttccctcat ttatagcaaa ttgccttata gcctcaagga aaggtaaact
     1621 ttctatatcc cctacagtac cgcctatttc tgttattacc acatcggtac tattggctct
     1681 tgctactctg tatattctgt ccttaatttc attagttata tggggaatta cctgtacagt
     1741 tccccctagg aactctcctt ttctttcttt gcttataaca gaaagataaa ttttccctgt
     1801 tgtaatatta ctgtttttat ttaaattttc atcgataaat ctttcatagt gccctaaatc
     1861 cagatcagtt tccgctccat catcagttac aaatacctcg ccatgctgat atgggctcat
     1921 agtacccggg tcaacattaa tataggggtc gaacttctgg atagttacat gcaatcccct
     1981 tgcttttaga agtcttccca aggaagctgc ggtaatcccc tttcctaatc ccgaaacaac
     2041 accgcctgtt acaaatatat atttcacagc catttattta ccctccaaaa tcttttgcat
     2101 tagtgtccca taactaatat attttatatt tacagttttt aagtgtcaat tcgagcagtt
     2161 ccatattata tggtttatac ttaaagatta ccgcctagct ctttgtttta aataataatg
     2221 caattttact aagggtgcat cgggcgcatc gaaaattttt gaagaaatat gcacccattt
     2281 actgtttcaa tatttctata gctttggaat attgcttgtc agaagtatta tatatccttt
     2341 caagctcagt atttaatgct ttctgcgttg taaaatccag tacaccaccg ggatacaacc
     2401 ctttatcagt gcggaattta gatattgcat cataagtttt cttatcgagg cggttgtcag
     2461 gagtgtcaac atcataccct aatattcgga gaattttttc agcattataa acatcaatac
     2521 cttcatcgcc aagagcaggc ttccaggtta ttgtcaactt atgtatattt tttaagtaaa
     2581 cgccttcaac tgtgggggtg tcttctacct gcacatcagg ggttattcct tttccatcaa
     2641 tcattctgcc gtcaggtgta acatatacac cggtagttat ttttgtccac cccaatattt
     2701 catctgttag aaggtttatg ttatattttg taatcagctc atatgcacct ataaacttgt
     2761 caccagttct ttcagaatac tttttatatg cttcaggagt aagcacaggc aacatacttt
     2821 gtacctttgc cttaccaaat gttttggtac ctacaataac tcccgcttta gtatcctgta
     2881 ttgctcccgc cactatttca gaagcacttg cactcatact atttacaaga acagcaaggc
     2941 ggtatttgga ttttggcaga tatgagtaat actcctggtc cttggtctgc tcagatttga
     3001 aaatcagttt agttatcagg ccttttggta caaactttcc cgccaaagca acgccttggt
     3061 ctacaagccc accaggatta tccctcaggt cgagaattat tttattaata cccatgtcgt
     3121 caatttcttt taatgcctgg ttaaggtact cttcagtatt tgcgttaaac atctcaagtt
     3181 ttatatatcc aatatcatct ttaatttcat aggtgatagg atttatctta attttttcac
     3241 gtcctacatc aatgttaata atttccacgc ttttatctct tattatccca agggtaacct
     3301 tcgtccctgc ttcacccttt ataagagctg ccacttcttc tgtagaagcc cctataatgc
     3361 ttttcccatt aacagcaact attttgtctc cctgaagcat accggctttt tctgcaggtg
     3421 atccggaaaa tacttttgat atcacaatag tatcggctat tttctccatt gacactccaa
     3481 tgccttcata tgaaccatct acatcaccga aaaacctttc tgcttcatcc cggttaaaaa
     3541 atccagtata ggggtccata gttccaaaca taccttttag ggcaccttcc aataattgct
     3601 cactatctac atcaccaatg tacttttctt taatcatttc tacaacacta tttaggtagt
     3661 cagaagggaa cccttcattc tccgaggcca aaacatattt catactggca gggtatgtaa
     3721 gtatcaggca aattataata atcatgatta taattctttt aatttccagg tacggatttc
     3781 taatgtaaaa cctctctttg tccactcaaa atctcctctt ttccttattc ataaatattt
     3841 tcaaggtatg cccatatccg ggattttatt ttacatgtaa atttccggta ggtattgtat
     3901 tattatctta gcaatcatac atatatattc ttattatata ttaaattaag tgaaagagta
     3961 gatgcatttt agaatattca tgttttgttt tcattttgca aaaacatgct tgcctattct
     4021 ggtaattatt ggtctcgacc atatccagct gctggtagca gtagctgggt tccaataata
     4081 tatacaccca taagtaggat cccacccgtt aagtgcatcc tgtgccgcct tatatgagct
     4141 gctatcaggt tcaatattaa tttgtccatc tgcgactgca tcaaaggcac ctggctggta
     4201 tattacacca gcaattgtgc ttgggaaacg gctatctctt actcggttta aaataactgc
     4261 accaacagca acttttccgg tatagggctc acccctggct tcaccatgta taattctggc
     4321 gagaatatta gtatctttat tgcttgaata tgccggcgcc tcacctgtag gaaggcctaa
     4381 agcagcaaga gtttgggaac ctgctatccc atctaccgcc aacccgtttt ttgactgaaa
     4441 atctcttaca gcctggtatg tcagtgaacc gtaaatccca tcaatatccc ctttataata
     4501 accccactgt ttaagctttg tctggatatt aataacttcc tgtccgctcg agccgtaata
     4561 tgaaagtgtt tcccggcttt tcccgggtat atcccctgaa ctgcttccgg agatactccc
     4621 tgagatattc cttgaaatgg tataatataa attatataaa taatttttgt aataaaatgt
     4681 aactcctgtt actattgcca atatcaaaac agctggtata atatgctttc tatatttact
     4741 caattttctc ataaataaag ttccactcct tttacgtata atacaaatta cttcatgaga
     4801 tattttctgc taattagctc aaaatattcg agtaatttgc tgatacattt ttaggaaagt
     4861 ggatatatgc ctcagagaac agatgttaga aatacctgtt ctctgttcta ttctgcatac
     4921 tatacttcca aagctacatt ctttctggag tattgatttt caacaaatca agtacatttt
     4981 taatgactat tcttacacat tcaactaaaa caagccttgc cttcataagt tcgtcctctt
     5041 cgccttttac tctgcaggca ttatagaaac tgtggaaact tgaagccgtg tcaatcacat
     5101 atctggttaa ccggcttggc tcaagcgtct gagcagatac ctttatttct tcgggatatt
     5161 ccacaagttt ttttataaga tcaatctctt cctgctcttt aagaagggcc aaatctacct
     5221 ggtcaacatc aggaacattt acaccttcac tttctaagag ccttaacatg ctacatattc
     5281 ttgcatatgc atattggaca taatatacgg gattttcatt tgattggctg actgcaagat
     5341 ccaaatcaaa atccaggtga ctgcctgaag ctttcatatt aaaaaagaac ctggctgcat
     5401 cttttccaac ctcttccagg agatccgcaa gtgaaattgc cttacctgta cgttttgaca
     5461 ttcttgctat ctcaccgttt ctatatagac gtactaattg gaatattacc acatccagct
     5521 tgtccgaatc aattccaaat gctttgacag atgccttcat tctacccacg tgtccatgat
     5581 gatctgctcc ccacagatta ataactctat caaaaccccg ctttaaaaat ttgctgcggt
     5641 gataagctat atctgaagca aagtatgtag ggaaaccatt atttctgacg aggacttcgt
     5701 ccttctcagc tcctaattga gatactttaa gccatacggc tccatctctt tcttcagtca
     5761 aacccttttg ctttaaaata ttaagggtct catccaattc cccactttca tacagagact
     5821 tttcgggaaa ccatacatca tatttaatac cgtagttttc cagtactttc tttatgtttt
     5881 caatattttt attcagcgca tattctataa gcttcccgcg tctttcagcg cattccgcct
     5941 caaggagccc agatccataa ttctcaatat agttgcgggc atgttcagtt atgtcctctc
     6001 catggtaacc atcctcagga aactcaatag catcctcacc gttaagaagc tgaatatatc
     6061 ttgcttcaag tgatatacca aacttttcta tctgattacc cgcatcatta atatagaatt
     6121 ctctggttac atcatatcct gccgcttcaa ggacactggc tatacaatct ccaagagctc
     6181 ctccccgtgc atttcccata tgcaaaggac ctgtagggtt ggcacttaca aactctacca
     6241 ttaccttctg tccttttcca atgtcgtttc ttccataatt gttcttttct ttttgtataa
     6301 ctttcagcgt gtaataaagc cattgattat tcaggtaaaa attaataaat cccggaccgg
     6361 ccttttcaac cttctcaata taggtcccat ttaattccat gttttcaata ataatcgatg
     6421 ctgcctttgc cggcggcatt cttaacagtt ttgtaatctg catggcaata ttggtagaaa
     6481 aatcgccatg gttcttttcc cttggaattt caataagtac ttccgggaag tccacctcag
     6541 gtatatcacc tttcgcaact gccttcaata tggcctgttc cacagtattc ctaactattt
     6601 gtctcatgtt ttccataaca tttatcattt gagcaccctg cctctcttat tgacatacaa
     6661 aagtcattgt atcccatttt attattatct atatcaatct gataatcaat agatatttca
     6721 ccaccactgt catctatttt aacattcaca ctattggcaa acactccaat tgtaaaagcg
     6781 ccatgagttg tatcataata tgaaatatgt ttttgccctt tctgaaatat aaactgggaa
     6841 ttaacagcac ctgctctttc aagagttaca acatcatcgg ctacttttat agtagtggtt
     6901 gtcccttcca tgcctgttac ttcgctttca tcatatgtta cataatatat attatcttct
     6961 ttataatatc tgccttcggt tacaagctcc atagaatcag atattccctg ttctgaattc
     7021 tgattgcctt ttaccgaaat aattacattt ttgtccatac aaacctcctt tttcttttgt
     7081 tatctaagct gtaaaagaat caatattttt ctatgtcaat tctttcagcg gaatttatct
     7141 ccatgcttaa aattgcactg cacaagggtt caaacttttc aacactatcg cttgtgtaat
     7201 atctatatac aggttttatt ttcccatctc tttcaatgca tgttttttct ataatacttt
     7261 ttacgactct tgcaacttct aatgcagaac ttacaagact tacctcattt cctactactc
     7321 tctgtataac tttttgcaaa agaggataat gtgtgcatcc caataccagg gtatcaatct
     7381 ctttttgctt tagagcgctt aaatattccg cagcaatttg gagtgtaata tcattctccc
     7441 accattcagg cccttcttca gctaaaggta caaaaagggg acaggctttg gaaaatactt
     7501 ttatggaatt atccagtctg tttatggctt tttcgtaagc accgctgttt atagtagctg
     7561 tagttccaat aacgccgatt cttttgtttt ttgtgtattt tacgcctgtt gccgcgccag
     7621 gttcgattac ctcaataaca ggaatatgga aactgctttt aaccatatca agacttaaag
     7681 cactggcagt attgcacgca attacaatca tcttaatatc ctgatttaat aaaaatctca
     7741 taccctgtaa agtatacttt ataactgtct cttttgattt tgttccataa ggaacccttc
     7801 cattatcacc aaaatataca atgctctctc ccggcactag attcattatt tccttcaaaa
     7861 cagtcagtcc tcctaggccg gagtcaaaca cacctataga cctattatcc attttttccc
     7921 cctttattcc gttttttcat tatatctttc aattgacagt tctataagct tgttaaggag
     7981 ttctccatat ggcagtccgg atgcttccca gagtttgggg tacatactta tttttgtaaa
     8041 accaggcatt gtatttatct catttatata tatctctcct gtttccttgt gtacaaaaaa
     8101 gtctaccctt gcaagacctg cacagtccaa tgctttaaat gcctttacag catattctct
     8161 tattttttca atagaacttt ccggaagcct ggctggaatt ataatttccg aagtactttc
     8221 gctgctatat ttagcttcgt aatcataaaa ttccttatca ggtataacct cgcctactgt
     8281 tgatgcaaca ggattatcat ttcctaaaac cgagcattct atttccctgc catttatgta
     8341 ttcttcaacc aatattctcc tatcatacat tcctgctaaa gaaatagcat caattaattc
     8401 tcttctgttc cgtactttgc tcactcctac cgaagaacct gagttgcagg gtttaacaaa
     8461 gcatggatat tccaatatat tttcaacttc gcttattatt ttatctgtat ctatctgaaa
     8521 ctgtcttttg ttaaaagcaa tatatttacc ctgaggtatt ccttctttat caaatactat
     8581 tttagcaaag gccttatcca tacagagagc agaaccaagc acttcacagc ctacatatgg
     8641 cattcccgca agttcaaaaa gcccctgaat agttccatct tcaccattaa gtccatgaag
     8701 aacgggaaat accacatcaa ccttttttat gtcattctca gcacctgatg caattaaaat
     8761 attcctggca gaattgctat aatcaacagt cccacctgat actgaacctg atactgaacc
     8821 taattctatt ttctccggcc tcagcctctg tgattccgca attgcctgcc attctcctgt
     8881 tccgattttc tctgcaggcc cgctatatgt gagccatttg ccttcttttg ttataccaat
     8941 cataactata tcatatttac tcttatccag gttttcgata actgactgtg cagaaaatct
     9001 ggaaacctca tgttctgttg actggcctcc aaatattacc gcaactcttt ttttatgatt
     9061 catattaagt aacactcccc cctgcaaaaa attcacatct agttctttaa acacacattt
     9121 atttttttga tatttactac tttgttatgt tgccaattaa catagtagta ttattttatc
     9181 agtagcattt attttattat acaaatttat tttatcagta atatttattt ttgccagtag
     9241 taatttttac tactttttaa tatatatttc acattctact atttatgaat tgttgtatca
     9301 agtttttttt gcatatatat agccatatag ccttcaaaat ttgccccata tctaatatca
     9361 tgatataatc atataatcta ccaaatttac cgataaaata tttggaggtg tacataaaat
     9421 gccgttctat gatttaaaat gcaaaaaatg tggtgaagag tttaataaaa tggccaaaat
     9481 aagtgaaaga gagcaaaacc ttattacatg cccgaaatgt ggcagtaata aattggatcc
     9541 catatttaca aatgttaact atgtagtttc aagtaagaaa gaaggccctt cctgcccaaa
     9601 tattgatcgg tgcggagggt gctgcggctt gaattaaatt ggatttttta agggaacaag
     9661 tgccgtgatt ttttagcatc tgttccctag atttatttat tctaaaattt cttcttctat
     9721 ttctcctgat gtttccttat cttctccatc taaggcttca tctgctgttt ttttactttt
     9781 tacaggaggt tctttgctat ttaaagaagg gcttccgggg aaaggggcat caaatttaaa
     9841 gttgcctttg aacttatctc tggtcttacc ttcaattgta aattttatct tttctatatt
     9901 cttcacctgg gttagggaat ttacaattga ataaattgtc attttttcag catcttttcc
     9961 cccaggatgg ttatctataa attcttttgt caggtctaca gttgctactt tcccttcaac
    10021 agtgaccggg gaacgcagct ccgtaccttc aggtattgta gcttttaatc cggtaccttc
    10081 accgggccct ttaattaatt gccttacaat ttcagatgca aggtctccag gatttttacc
    10141 atctccttca agtttaatat acctgatttc tgtcattagt tttgaattgt cttcatttgt
    10201 aaagtaaagt cttacaggta ttttttcgga aattctggct gcttctattt catccatata
    10261 tacaaaactc acaggatgaa tttcatcaat atcgtttttc tgaaatccaa gcttttgcag
    10321 gacactacac ccgctaatta gtgctactac caacacaagc atagtggtta tacttataat
    10381 ttttacataa aatactttac gcattgcccg gcacctcaca ttattgttct tataagatat
    10441 ctttatgcca ggtacatttt tagtattctt taaattttaa atattttatt gaaaagtaag
    10501 tttcactgat tcaatttact ttatttgcct attaatatca aaattgacgg ctggtttata
    10561 ttgcgctgat tcacattgcc ttggtatatt tatctgcaaa ggaacttgca caataactat
    10621 ccggctttat cttgggcaca aagctcaagc cctttaccat gccggtcatc tcactaatca
    10681 gggcttttgt agggccttta gtcccaatat caatatgaaa ttcgaaagaa acacctcttg
    10741 atttatcacc agacttgacg gtattaataa tattctgtat tttgtcatca tcaaacaaat
    10801 aagccagttg cagacttgcg catgtttcca gatatatttt ttctcgtaaa ctttttattg
    10861 gccttggtat ttgagcttta tgaagaaatc caattgcacc tctacctaca cggtggacaa
    10921 gaatgcagga cacaaaaaca gtccccgttc ccgattgtga gtcggttcct atagatattt
    10981 tatatgaaca ttccgtatca gacctgatga aattaagtat ttcatcataa acatcactaa
    11041 tggtcatgtt atctttccga atactccgaa aaactgtgtt ttcgtcaaac tcaagagcgt
    11101 tccccaatat catatttctt cctccgttgt taagctatac accactgcct tttctgaatt
    11161 tatataatta aaattgtaac acatacattt taaaaaagca ataatccaaa tttttactat
    11221 cctaatacta ctttgttatg ttgccaatgg aaagcgcatt tctggagggt aattttttct
    11281 gacatttcat gaattcactt gaaggaaggg tcgcaaggac ttctgtagaa acatttctcc
    11341 gtgtgacatc ttccttatgc tatcaaaaat tatcctggga gatattgctg tccaaattgg
    11401 catagtaatg ctaataaaaa aagaacacaa taatgattga agtaatgccc caaagtataa
    11461 tattgattat taaaggcagg tcttttagca ggacaatttc cgggctgccg ccatcattcc
    11521 cggaagatac caaatattga tatctgaata tgccatacag tacaaaagga atagttaaca
    11581 tcatgtaatg ggatttacca gaagtaaaag tatataggga gtatgacatt attgttgctg
    11641 aggaaactac cgtcaacatt tgatctatca gttcaggagt atactgttcc aaatttttcc
    11701 tatgatttgc agcatcttca gataatagaa taagctcatt tttacgctta ttcagagcta
    11761 aaaataatgc aagaaaagtt gtgcacataa taagccatgg agatatttct actcttatta
    11821 tggccgttcc tgccagtgta cgcaaaacaa accctacaga tattatgacc acatcaagaa
    11881 ttacaatatt ctttaaaata attgaataaa ataatataag caagaaataa gtaaatataa
    11941 taataaaaaa cagaaaatcc aatttgtaag atgcaaaaat aactgcacag agaataacaa
    12001 gcgccggaat tattgcttca tcaggtttta atctgcctga tgcaataggc cgttttgcct
    12061 tcaaaggatg aagcttgtcc ttatctctat caataatatc attaattatg taaactatac
    12121 cagatactaa tgaaaaaagt ataaaagcat atattgatct taaaatcaat gctaaattca
    12181 taaaagattt tgaaaaaatt agtcctgcaa aaacaaacgc attctttatc cattgttttg
    12241 gcctcatagt taatataaaa tcctttactt tttgtctaaa tcccaggtgc atattttgct
    12301 gttttatcat tagttaaatt aaccgccttc ctaaaaaagt tacttctcta ttttgtatat
    12361 tataccatca taaactttct taatcatttc aacattaaaa aaatttgcca attcctcagc
    12421 attataaata aagttgccag taccaacatc aaaccaggca acatatacat tttcagcttc
    12481 gtaaacaatc tttttaaatt catcaagtcc atatatatct ggtccctctt ttttaggggt
    12541 gtacatggat tttcggcctg tataaaaata aacagcgtca gggcaatttg tgtaaatagc
    12601 actattctga ggtagttcct tcacataggc aatcaaagga gattgggccc accatttgga
    12661 ggcaagaata cctgctccat tttcataagc gtaagcagca tctgtactaa tttctgaaat
    12721 aggatatata atccatgaaa taaagataat aacgaaaact aatacagtga tgattttatt
    12781 tttcttccct ttatctgttt tcacaatata tgcaatagaa aaaaactgca gcagtacaag
    12841 agggatgtaa gtgggtacca gcaatctgtt atttatgctg tcgaaggcaa caattgacgc
    12901 agataataat aaataagcaa tatatattac aggaaatata agtagaatag ccagtctgtt
    12961 tgatttttga tcagagtggt ttctaatagc aataatagaa gctataaata tgaaaattcc
    13021 caatatggaa tatgccatgt tttcggagcc agcaggtaca aaccaagcag caattatttt
    13081 aaataaagtc ccggcatttt cacttattgt attttctgac ggaggacgcc ctcctgtgag
    13141 agttcctgtc agcatatagt tccttgccac ccatatggac gtaggaagag cagatataaa
    13201 tccatatatt actatttcta tgaaacggtt taatattttc tttttctgga atagtaatat
    13261 aattatgcct gaaatgatta gcgttatgcc cacatacctg gtcaaacacg caagagcagt
    13321 acatattgaa gctcctgcca tatattttgg agaacttgtc cggatatagc aatcaaggtt
    13381 tatcagaaat aaaagggtca gtaaaataaa taaaggctcc gaccatataa atcttgatac
    13441 atagtataaa ggtattgatg caagcattgc aaaagatcct gctactgcaa ttatgccgtc
    13501 ttctgtacat ttaagcagcc ataaacccga aatcaggatt attaatgtaa aaaccaggct
    13561 gttaaaaaaa cctgcagcga ctattacatc agcccctgtg ctttttaaac cggcgaggat
    13621 tagtgggaac agaggcggcc attgaatgag tggagtatca tatgcaaaat agatgagacc
    13681 tctacctgac aaaaagcttt cagccgcata aatataggct atggagtcat gactgattcc
    13741 aggcccatat tttgaaatac tatatgaaat aataagaaaa ccacatacag aaagaaaaat
    13801 taaaaatggt agagtcgcat atacactgac cttgtaattt ttacctctga tttcatcggt
    13861 cttacccatg attccattgc ccttacccat gattccattg gtcttgtcat catgactttt
    13921 actgcacaga tttttgctgt cttttcgttt aaaatttccg ctcacttaat caccttgtcc
    13981 aacagttatt ttatcataat taaataatac ataactatat attacgttgt tttcatctgt
    14041 tagacaacaa ttatcttata tattctataa tctttcttcc tgccaaagca ccctggccta
    14101 ctgctgtaga aatttgttta aaacctcctg tgcaatcacc tgcagcaaat aatccttcca
    14161 ggttggttcg ttgttcactg tcaacaacta ttgaatttcc ttcagtgata acccccagct
    14221 tcctggcaaa gtctatactt gatgcgcttt catttgcaat aaaaagacca tccaggtcct
    14281 cccatgtttc gtcagcaaaa catatccttt gaaggtaatc agctccgttt atttcggtta
    14341 tagattttgt atttatttga tatttagcta tctcaccgga atacttctct gttagatcaa
    14401 gctcttttcc gtttgtgtat attgttatgt ctttagtata ggcttctaat tccattgctt
    14461 catgaacagc gtaatcttta tttcctagta cacccacttt gaggttgttg taaaaataac
    14521 catcacaggt tgtacagtag cttactccat ttccctcaaa cttttccatg tttttgattt
    14581 taagtttctt ttgtggctga cctgtggcaa ttaatactgc tataccctca taaacttcct
    14641 ctgaagttgt tacctcaaag gcatcgcttt tctcaatgga tacaacttca ttattaacaa
    14701 tatctgctcc aacattaaga gcctgttttt ccccttcatc tagtagaaac tttccgctta
    14761 caggactgga aaatccaaag taattttcaa ttttcgctgc ttttccaaga gcactgtcat
    14821 ttttgcctaa aattaaagtt tttaaatttg ctctggcagt ataaagagct gcagaaaggc
    14881 ctgcaggccc ctttccaatt ataataacat catattttaa catatacatt accccttctt
    14941 atttcatttc ttccactact tccataagtg tcttttcatt agtagcacca gatatataag
    15001 cgtaaaaagc accttcacta tcaataataa aagttatcgg aatgcctgaa acaccatatg
    15061 cattactgat gctgccatcc gtgtcaagaa gtacgggaag agaaagcttc ttttctttca
    15121 taaactcctt aactttgtcg gcactatctc cgctgtttac agttattatc actgcatctt
    15181 ctccttcaag gaatttcttg ttcgcagagt tcaagtcagg catttcctga acacaaaatg
    15241 gacaccataa agcccagaaa tttaaaaaca caacttttcc cctgtagtca gtaagtttaa
    15301 cctttttgcc ctccagatct acaagttcga aatcgggagc cattattttt tcttcctgct
    15361 gtttggaatc ctggtcagta ccatctttct gtccttcctc aacttgctct tttccatttg
    15421 ttttatctgc tttctctgtt tcttcggttc cacttgtagt atctgttccc tttgtattat
    15481 ctgttccctc tgaattctct atttttttag tctgtcccgg agccattttt ataggagttt
    15541 tacctgcata tctattatat aaattatata atattactgc aatcaatact gacattacaa
    15601 tccaatacaa tacatttttt agttttcccc aattaacacc ttttgcgctc ataaaagtac
    15661 ctccttaata tgactttaat ataacttttt tatacatatt ttagcaaatc tgcgtataca
    15721 agtattcccg cagctattaa aataacccct gaaacaatac ttattattct gcctttttcc
    15781 tgaagccatc tgaagctatc tttaatttcg ctaaataaaa gcgaagataa tataaatgga
    15841 actcccaaac ccaatgaata aatagaaagt agaaaaaccc cttctttaac agtacctgaa
    15901 ttacttgcca taagaagcac tgagcctaag aaagcccctg cacacggagt ccatccaaag
    15961 ccaaaaatca tgccgaagac tatggagctt aaaaatttca gttccttaaa tttgtaatca
    16021 aatttttttt cagtatttaa aaagtctatc tttataattc ctgtaaaatt caaaccgaat
    16081 attatcatta gtataccgct gatcttccgg aataacccaa tattatcatt taaaaagtgt
    16141 cctatggttg tagcggcagc accaagtgta ataaaaacaa ttgaaaaccc aagaacaaaa
    16201 ccaagggaat taaacaaaag tctgtatcta ttttttactg cattattttc tgccgaaact
    16261 cccgccaaat aaaaaaaata tacgggtaaa ataggcagaa tacaaggtga aatgaatgta
    16321 agaatacctt caataaaacc aataatataa gaagagcctc ccatagtcca tcatcctttt
    16381 caacaaaata ttttcatatt ttatataccc ctgcagggta ttatataatc atcaaataat
    16441 cattgtcaat attatgtctt gggcacatca tctatatttg aagaaaatgc acctttgcac
    16501 actcttactg tacgttgaat attatacaaa tagaaaagca ggcgtagaag gagatgaatg
    16561 aacatgctac cacctatctg taaattcaaa gaaaatggca gtcgtaacaa ctcagaactg
    16621 ctttatttta tattaattta cctgttgctt ttttatgata gcaggccttt agctatttta
    16681 gcaggaagga attttaacaa cagcctaaat ccgtattttt gtaatgatga gtgttgcaag
    16741 tgtagctgcg ggtgtggtta cagtagctat aacatagcag gaaacaaagt cagggattca
    16801 aaaatgctgt attttgtttt gatttttttg attctttttt attagcataa ctactgcttt
    16861 ggacacaatc atcatatctt ttttaaatta tagtaacaat tttaatatac tactcataat
    16921 atagtatgac agcatataat gggtggtgtt aaatggatat tttgcaaaat tcattaacaa
    16981 atttgagcag atttttcagt gaggggggtg atgaattact aataccttta ataattttat
    17041 taactcaatt taaagatgac aaggaggaaa gatctagcgg cctgaagagc tttgatttta
    17101 attcaatact cttttttgga gcccttttct ttatactgtt cattagcagg tactttgaaa
    17161 aagaaatgac aagagtaaac ctcgaggaaa tctcgtagat aaccgcaata tatggtgtca
    17221 ttaaaatcat gcaggaaata ataaaaatat tatattaacc agggaggatt attataatgg
    17281 gtaaagactt taataaaatt tttgcagaaa tgctaggcaa aatggatgag aaagttttgc
    17341 aggctaaatt gaacgccgct cttgatatgt taaaaaaagg caagactgat gacttggcaa
    17401 aaaaaataaa taaaattgat aaaaacgaat taatggaaaa aataaacgaa tttgatgaag
    17461 aaaaaataaa agacatgaat ataaatttag atgagattaa aagcaaggtt acggaaaatg
    17521 accttgaaaa tttaaaatct ctggtaggtg aacatggaga tgagattata gcaaaaataa
    17581 aagacattat taagtaaatt ctgaatatat ttttaatatg tataatagtt gctaacctac
    17641 aggaggaaaa aaccggtgga cgatgatctt ggtaaaacaa ttaaacaaat aaccgacatg
    17701 ttgagcaagg ataaggataa gatgccagat aacctcatgg gtcttatatc tcttctaact
    17761 tctccaggaa gcagtgaaag tggatctgag aatgatgtag agagagaaga aaagaatgaa
    17821 aaagagggaa ataacgtaaa ccggagtaat aagggcgatt tacaggataa cctggaaatg
    17881 ataaagcaag taactaaaat tatggatagg ttgaatacaa aaaacgatcc aagagtaaac
    17941 ctattaaatg ctttaaggcc ttttctaaat aacaaacgtc aaaaaaagat taataattgt
    18001 attatgctgc tgcaggtaac aggtataatg aaggcaatgg atgaataggg aaggtgactt
    18061 ggatggcata tagaagatat ccttatatac atagaaatta ctactatacc aggaacttac
    18121 cgaatggaaa aaacaatgcc cctacaaagc tggttaatga tatccctatg cctaatgaga
    18181 aggaaagtat acgcgacccg gaggtccctt taaaacccgg gtccaaaact tcttcgaaag
    18241 ccagttcaga atcctctttt aaaatcggac atgatagctc caataataac atacctgata
    18301 cacctgataa taaaaaaagg gactataagt ccccttttat aaaacttttt caagataaaa
    18361 ttcaacttga tgatataata atactgggtt taatatttat ccttctctat gaaggaataa
    18421 aagatgatat gctatttcta attctaatat acatcctgct tggataatat tatcacatgc
    18481 ttactgcaaa ctttcaggat tattatcaga atattttcta tatgctgcaa atatatacgt
    18541 tgtataaaca gcataaaata ctgaagaaaa ggcccatttc gctaaaagaa ggataatatt
    18601 gtcatccagt ttaacaaaaa atatctgaga tataatgtaa actatatcga aaacaacaaa
    18661 aaccgataca atcctccaaa aattattgtt tgcaattctt ttactttgtc tgaatggtct
    18721 tttttcatta ttcatagctg caggatacca gaaaaaagtg taaatcctat aaaacatcaa
    18781 gccaaagaac agcactactc cggtcaggac atcaacaaac aaaactgctg gaagcatatc
    18841 ccttccttta cctgctgatg cccttgtaat aactaaagct ggcacagaag caactactat
    18901 aattatcagg ataattaacc ctgtagcaag cagcacaaaa ttcattaatg ttattttatt
    18961 gaaatactgc cttactccat gggaaagctc tccttttttt ccggcttttt tattaaccgc
    19021 attattcagc ttgaaaaaat agcctgatat taaggctcct gaaattacag ctattgacag
    19081 taatactaac aataaaacaa ctgctgctat aataacaact ttaatgtcca acatatagct
    19141 atatacaagc tgaataaaag acattattgt ttcaaaagga ttgcttccac taatagtatt
    19201 tagttgaaac agtgttctga atacaggaag aatataccct gcaacagtgc aaatcagcat
    19261 taatattgcc caaaatatta ttataaaagg ccttttcaat atgaccccta tagcgttccc
    19321 aatagattcc atttaaccat tcctctacct atatagaatt taagtatatt ttaccagtca
    19381 tgtattttga atgcaagcac ttatcgcaaa ttcccccaat cgtacctcaa agtcactttt
    19441 ttttcaactg ttctgcagtc tgctccagct gctccaatga tgctccatcc ttaatcatat
    19501 tataaaattt aacaacaatg ttaaataagg ggctctcgct atctgtacag ctatattggc
    19561 cgagagcttt ttcaatacct tcagaagcaa cagtctgtct tacctgagat gcaaatctat
    19621 catcttctgg agcaaatata tatccggcag caattccaat gcatatatat gcaggaacaa
    19681 taccctgttt cacgcaaagt tccgctgcac ctaccagcct gtcattttta gaaagctttc
    19741 taaccgtatc tctgccaacc ctggctactg tatctcccaa taagcggttc ccaaatctgt
    19801 ataataaatc ttcagcatgc tctacaagct tttgcaaagg ataattatgt tcaagagata
    19861 aagcaataga agattcaagt agagctttca ttgttattaa tttaatagca gggtttttgc
    19921 atgtttccca tatatatgta taattattaa ggaaaccaag atatgcagct gttgcatgac
    19981 tcatattatg cataaaaagc ttacgttgaa tatataaatc aaatggagaa aatggcacca
    20041 tatttatgat agaaggtatt tctcctttaa aagcatcttt ttccacggga agttcacaat
    20101 attcctctac atataccctt aaggggttgc cttcctgcat ttcttcagtc attacaggca
    20161 ccattcttcc tatagaagct tcaacaaggc ctaccttttc atcaaataat ttgttttctt
    20221 catcattaag ttcctgtttt attagttttt ccaaatagcg gttagcgtca agcatatttt
    20281 cgcatattat aatattaagt ggatccatgt ttttgccctg ccaccgtttt ttcaatccca
    20341 tagatatagg tcctgcaact ttaggaagta catttactcc tacagccgta gccataatat
    20401 cagcctctga tatctctcgt gctacgcttt caatatcagt tccatttacc cctctcacat
    20461 ttcttattac tatttcatta taggtatcat gagcaacaac ccttattggg taagctccgt
    20521 ctgaattgag tttttcaata acaatagggt ttatatcgat aaaaaccact tcatagcctg
    20581 actgactaaa aagctgtcct ataaatcctc gtccaatatt ccccgcacca tacattacgg
    20641 ctttcttcat aatcactctt cctctcatta tataaattcc ggtataaatc ccggtaattt
    20701 ttctttaaga atttctttag aaattttata aatccctata ctttaaaatt attaaccctt
    20761 ccataatttt atattatctt tttattatac attattttat taaaataggt aaatagggtt
    20821 gaaacatgaa atattatgat atatatggac gaaacagtca atatgctgaa aatgctccgc
    20881 tttttataca aataatacat aaaaaataaa ccacatcagc cggaaccgta tccagacata
    20941 aatgtggctt atttagcctc attgggcatc acattatttg gtcacagatc attcaagtaa
    21001 attattttta atctcaacca tcaatattta cttgttgtca tagttttttt caaaaccttc
    21061 atgagcattt tctcccagct gatcattatt aaatgatggt ttttcctttg ctttaacttt
    21121 tttgttttta ctccttttgt caggcatggt tataacctcc tttcaatctt gcttttttat
    21181 caggctttat aagcctaaat ctttataaat agttgtataa cttgagttat cttgagttat
    21241 cttgagttat cttgaaccac cttgagctat atcacttaag ttgtttagct tggatcgttt
    21301 tcgtccagat aaacttttgt tacttttgtg taattacctt ttaatatatc ccttacagat
    21361 gctctttttt ccagggcatc attacttaat ccgggggcaa atacaggtcc gtcattggta
    21421 ttcttctgtt ttcttctttt tatttctttt ctcatctgtt atatctacct ccatgctaat
    21481 atgatatcca aaaaaatgaa gcctatacgg gttcttctat agttttgcag ttttggtgca
    21541 tttgtatgaa gtgatgaaaa atatggcaag agacgatttc caagtataat attcacttca
    21601 gtaacggatt gtagtgttta agtctacaga aaaattctac ccaatcacat tcaccatcca
    21661 tggttcaagt gacggctcac gacatccgtg tcgtgaatta cgaatttttc cttgtctcaa
    21721 acataacaat ccgtaaactt tgctcaaatt ttatatttgg aaatggtatg ctgccatatt
    21781 tttcatcaag taagaaaata tgtccccttg acctttaatc aatttatttt ataattatta
    21841 tcatgcaacc taattaattt aagaatcgga gtgtctgttt aatgaaaaca gttattgtag
    21901 gagacaatca aggaaatagg aaaatatcaa aatttataaa agacaacttt ggaagtatgc
    21961 ctgtgagtgc catgtataaa gccttcagaa agaaagatat aaaagttaac gggaaaagag
    22021 tttctgaaga ctatattgta atgcccggtg ataagcttga tatttatatc cctgataata
    22081 ttttaaatgg aactcctata ggaaattccg gccttttgtc cagaaactcc catgattgct
    22141 caactcgagg cttttcagtt gtatatgatg acagcaatat attaattgtc aataaagatc
    22201 agggtatacc tgttcatcct gatgtaaacc aagaggaaga tactcttatt gattcggtta
    22261 aatgctacct tcaatggaag ggagaatatg atcctctgga cccttcatct ttttctccca
    22321 ctctctgcca caggcttgat aggaatacag gaggtcttgt aataatagcg aaaagcagca
    22381 taagtttatc tattatactg gaaaagataa aaaacagaga aatcaagaag tactatcaat
    22441 gtctggtaaa aggtaaaatg gaaaaagagt gtgatgaatt gagagctttt cttgaaaagg
    22501 atgaaagaaa cagcagagtt tttattaata atacgagaaa acccggttct cttgagataa
    22561 ttacaaagta cagggtgctc tcttatgaaa atgatatgag taagctggaa gtagagctgg
    22621 ttacagggag aacccatcag atcagggcac acctggcttt tataggtcat cctgtagttg
    22681 gagatggtaa atatggcata aacagctata accgagctct tggagtaaaa aagcaggcac
    22741 tttgggcata taaaatttta ttcagtatga aaaaggattc tggtatactt aactacttaa
    22801 aaggcaaggt ttttgagata gaaccgcagt ttacaatata agacaaaggt atactcctta
    22861 taaggtagaa gtgagaagta agaggtggga ggtgggagat gggaaataga atagattttt
    22921 atttcttcat taattctaac gactcgcaac ccacaacccg caatcaatct ttactctcta
    22981 acttctagtt tctacctcct atatctagct cctcatctcc agcctcttgc cttttaactc
    23041 taatttctaa cttcccattt cccacttccc atttactatt tcccatttac tacttcccat
    23101 ttactacttc ccatttacta cttctatact gaggaatctc cttaatgaat cttattttgg
    23161 tttttatctt ccaataaaca tctgtacaag tattttgccg tacttggaac tttcaactat
    23221 tcctataccg gtatagttaa aacttccatt caaaatattc tttctgtggc cttccgaatt
    23281 catccatgct ttaaatgccc catcgacagt tctgttgcct gctatatttt caccggcagt
    23341 cctgaagcta atattgaatt gcctcatcat atcaaaagga gacccatatg ttggtgaatc
    23401 gtggttgaaa taatttttat caaccatatc tttagctttc aatctcgcaa cttttaataa
    23461 ttcagaatca aactttaaag gtcccgctcc tgcatcagcc ctggctttat taaccagatc
    23521 cagcaacgct ttctcatctg ctgttgcatc agaaggcggg gccacttctg gagttggttc
    23581 cggttttggt tctggtttag gctcaggctg aggtggctgc ggagttggct gtggggttgg
    23641 ttttggctcc gctttgggcc atgctatatc cagatatttt ccatgagcta ctccaataca
    23701 acccattcca gactcttgta atgcatacca atctccaact ttagcaaaaa ctttaacact
    23761 ctggcccttt ttcaaaacac aaataacttt atgatttgtt gacggtccct gccgcacatt
    23821 caaaaagttg gccgtgaccg ttgcatcaag gtatcctaat actgatatgg caggctctgc
    23881 ttttacactt gtaatattat cagcaaaaaa gctaaatgcc gatgtaaata ttaaagcaag
    23941 caccattact aaaattattc tttttttcat ataatccctc caacaaactg tgtaatcttg
    24001 taaaacataa ttataactat agtattgaca tttttttttc gaattattta catttataaa
    24061 ctaatggaaa tataaatatt ttattttagt gcaaaaaagt gcccttgggc acttttcaca
    24121 gtttagttgg agttatttgg ttgctggtaa attgtatatg ctgccttttc tcaattacca
    24181 actgcctttt ttacctcatt caatatattc tcaaccttaa tctcttttat ttctgaagat
    24241 tttctgagtt tgtactcaac aattccgtcc tctgccttac gtcctacagt aattcttaca
    24301 ggtataccta ttaaatcagc atccttgaat ttgactcccg gcctttcatc tctgtcatca
    24361 atcaaaactt caattccatt gtcagcaagc atgttataaa tttgctctgc aagtttggcc
    24421 tgtactttgt cggattggtt aacaggaact actattgcat ggtatggtgc aactgatgca
    24481 ggccatataa tcccatgttc atcataattt tgctcaatta ttgcagccat tgttcttcca
    24541 acgcctattc cgtaacttcc catgatcata acctgctctt taccattttc atcaaggtat
    24601 acacaattca aggcagtgct g
//
LOCUS       DAOSDD010000033        24461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_916_length_24461_cov_5.039089, whole genome shotgun sequence.
ACCESSION   DAOSDD010000033 DAOSDD010000000
VERSION     DAOSDD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24461
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_24461_cov_5.039089"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK733_04820"
     CDS             <1..270
                     /locus_tag="PK733_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOJ09899.1"
                     /translation="ITEICRKAEILVAAAGKANMITKDYVSPGTVVIDVGINVDDKGN
                     LCGDVNFDSVADIAGSITPVPGGVGTVTTSVLVKHVIKAAVAALK"
     gene            328..1302
                     /gene="hflK"
                     /locus_tag="PK733_04825"
     CDS             328..1302
                     /gene="hflK"
                     /locus_tag="PK733_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOJ09900.1"
                     /translation="MVKKISKFLIVAIILVIAGIWLSSGFYTVKSGEEAVVLKFGKHV
                     GTVSKAGLNWHLPAPFESVIKVNVLEVKRLEFGYKTIKEGGRGADSDYADIAPALMLT
                     GDENLVNVEAAIQYKIKEIDDYVFGVKSHDETLQIAAESAIRRVIANHILDEVLTENK
                     YIIQQEIKEDLQKTCNDYKLGIGIIAVQLQDVNPPDEVDAAFKDVANAREDRNSYINE
                     AESYKNEVIPKARGNAAKMVNDALAYKEKRIAEAKGDVANFAQILEKYEQGKDVTRIR
                     MYLETMEEVLPGIDKYIVDSQGNLIKFLPLEQSQTLQNGSKQEKGGSN"
     gene            1304..2329
                     /gene="hflC"
                     /locus_tag="PK733_04830"
     CDS             1304..2329
                     /gene="hflC"
                     /locus_tag="PK733_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOJ09901.1"
                     /translation="MNDNMGNMGNSNNMGFGQQRYNFNLSNFKGFYKIAGITIIAVIL
                     LTVISNMFFFSVDEREQVVIKQFNEVVKIVVDENVDKIREIASENPQLRDIKIDNRKG
                     LHFKVPFLQTVESFTSMLLTYDTEVREVITKDKKKLVLDNFAVWKITNPALFSISLRN
                     ERAAHTRLDDIIYSKLNEEIGKVEAHVVISDKNFVEEMLDRVIQSTNKQVESYGMEVV
                     DVKIKKTDLPVANYSNIFNRMKTERERSAKTYRSEGQEEAQKIRSEADKEATIIEAKA
                     YEEAEKTKGEGDAEALRIYAEAYNKDPEFYAFWRSLQAYKKTLKDNTKIIIDSNSEFA
                     KYLYNIK"
     gene            complement(2449..3513)
                     /locus_tag="PK733_04835"
     CDS             complement(2449..3513)
                     /locus_tag="PK733_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09902.1"
                     /translation="MLINNKNRDKENGDNANISIKNKLLNRMIAFALIMAFAITILIS
                     GCNREEKGPSAQRQSGESEGESGKIPGELTEMEQGIEKIIKALNGPSAPETEKEESGK
                     KGAEEEKQGSSDQTKKQDTEKEDGAKSEDKQSDQGKQQGQQGQEGQGKQQQTQQTETG
                     AKKPQEKDPWQEVTPIINKMHFTWNSYMPEAVQKGASQEIINGFSSSLNGLTNSIISK
                     NKNYTLISANNLYGNITGLFSLYKKQNVSEVKRLKYFTRNVVLSAMNSDWTQAETNIN
                     NLKSVWGFLKNMLGEENKKAASKLDFSITELEKVIKEKNQPLTDIKGVITLSNIEEVD
                     KNLEKASSQSSGQGKEKSSK"
     gene            3916..4488
                     /locus_tag="PK733_04840"
     CDS             3916..4488
                     /locus_tag="PK733_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ09903.1"
                     /translation="MSAYDIILRRRTIRKFKQDKIERELLVKLVNAARHAPSGANMQP
                     AKYLIIDDSNTVKKIFEHLKWAGYIAPHGDPKEGERPVAYIIVLADTRIRKSGYELDL
                     GAAIQNILITAEEENIGTCWIGSVNREEVRQILDIPDELLINSVIALGYKAESSVAEE
                     ENGSIKYYKDNKGILHVPKRKLKDIMEVSR"
     gene            4485..4772
                     /gene="gatC"
                     /locus_tag="PK733_04845"
     CDS             4485..4772
                     /gene="gatC"
                     /locus_tag="PK733_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOJ09904.1"
                     /translation="MSFSKETIDYIAELSRIGLSEKEKEDMVAPVEKILACMDKLEEL
                     DTTNIEPMEHIVSAVNVFREDKVEKSYDRDIILANAPEHEDGCYSVPKIVE"
     gene            4787..6259
                     /gene="gatA"
                     /locus_tag="PK733_04850"
     CDS             4787..6259
                     /gene="gatA"
                     /locus_tag="PK733_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOJ09905.1"
                     /translation="MKLYELTVSQLSSLLKKKEINAVELTRTVLDRIVEAEPVLDCYI
                     NIIEEQAVERAEKVQKMMDAGELYSPLAGIPMAVKDNICTEGIPTTCASKMLQDFVPP
                     YNAAVMQKLYNNGAILLGKLNMDEFAMGSSTENSYFKKTKNPWNHDRVPGGSSGGCAA
                     AVAAGEAIYSLGTDTGGSLRQPSALCGVTGFKPTYGSVSRFGLIGYASSFDQIGPVAK
                     DVTDSALVLNAIAGHDSQDSTSAGIKYPDYTESLVNNVKNMKIGIPSEYMRNNTSEDV
                     RKAVSEAIELFEKLGAKCEEFSLPIMEYVLPTYCIITFAEASSNLGRYDGVKYGHRAK
                     DYKDLGELYKRTRDEGFGSEVKRRIILGTYVLSAGQYDKYYKKALQARRVICHELNRA
                     FDKYDLIMGPTSPSTAFRFGTKVEDPAEMYKNDVLTMPANIAGLPAISVPCGFDGDSM
                     PIGLQFIGKQFGESTLLRAAYTFEQNTDFHKNKPVIKPVI"
     gene            6274..7704
                     /gene="gatB"
                     /locus_tag="PK733_04855"
     CDS             6274..7704
                     /gene="gatB"
                     /locus_tag="PK733_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOJ09906.1"
                     /translation="MNYEVVIGLEVHAELSTNSKIFCSCTTEFGGDPNTHTCPGCSGM
                     PGSLPVLNRRVVEYAIKAGLATNCIISNYTKMDRKNYFYPDLPGAYQISQYDIPICKN
                     GYIEIEVEGKKKRIRVTRIHMEEDAGKLMHDEFGAGSLIDYNRAGMPLVEIVTEPDIR
                     SAEEAKAFLDNLKSILQFIDVSDCKMEEGSLRCDVNISVRPEGEEKLGVRTEMKNLNS
                     FKAVISAIEYESKRHIDEIESGGVIYRETRRWDDDKGFSYSMRSKEQAEDYRYFPDPD
                     LPPIIIDQEWIEKIRSTLPELPEARKQRYMKEYDLPAYDAGILTSSKVISNFFEKALR
                     KTDNVKAVSNWIMGDLMRILKDRKLEVENIPFPAEYLADMISLIDKGTISGTMAKKVF
                     EKMFDSGKAPEVIVKEEGLEVVTDEAAIAKIVDKVLDNNPGSVADYKAGKKKALGFLV
                     GQVMKESKGKAEPQLVNRLLSEKLNE"
     gene            8046..9038
                     /gene="pta"
                     /locus_tag="PK733_04860"
     CDS             8046..9038
                     /gene="pta"
                     /locus_tag="PK733_04860"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOJ09907.1"
                     /translation="MNFLERTIERAKSDIKTIVLPESTDIRVIKAASIALEKGVANIV
                     LVGNREEILSKAGSLDISKAVIVDPLKSEKYDDYVNTFYELRKAKGITVEKAQEIMKD
                     PVYWAVMMVKKGDAHGMVSGAVNSTANTLRPALQIVKTAPDAKLVSAFFLMVVPDCEY
                     GHNGTFIYADSGLVENPNEAELAEIAITSAKSFKLLAEAEPVVAMLSYSTYGSAKSEL
                     TEKVIKATKLAKEKAPNLLLDGELQADAALVSSVGKSKAPGSPVAGKANVLVFPDLNC
                     GNIAYKLTQRLAKAEAYGPITQGLAAPVNDLSRGCCAEDIVGVIAITAVQAQKE"
     gene            9165..10361
                     /locus_tag="PK733_04865"
     CDS             9165..10361
                     /locus_tag="PK733_04865"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOJ09908.1"
                     /translation="MKKVLVINSGSSSLKFQLIDMSNEAVLAKGLCDRIGIENSFLKY
                     TKYGEDQVIINKDMADHKAAVQEAIHALVDKKTGVISDMSEISAVGHRVVHGGEAFHE
                     SVVIDDEVMNAIEECINLAPLHNPPNITGIKACQQIMPGTPMVAVFDTAFHQSIPKYA
                     YLYALPYDIYEKYGVRKYGFHGTSHKYVSQRAAAILNKPLEELKLISCHLGNGASICA
                     IKNGKSVDTSMGFTPLAGLAMGTRSGTIDPAVISFLMEKEDMTIDEINEYLNKKSGVL
                     GISGVSSDFRDLEAAADAGNERAKLAIDIFCYRVKKYIGEYSAVTEGIDAVIFTAGIG
                     ENNYKVREQILEGMDYLGLKVDHEKNKIRGKEIDVSADDAKVRTLVIPTNEELAIARE
                     TMSLLK"
     gene            complement(10591..12342)
                     /gene="pyk"
                     /locus_tag="PK733_04870"
     CDS             complement(10591..12342)
                     /gene="pyk"
                     /locus_tag="PK733_04870"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOJ09909.1"
                     /translation="MRRTKIICTLGPAVDDENILSELMLKGMNVARMNFSHGTREEHK
                     QRVERFKKVRDNSGLPVALLLDTKGPEIRIGKFSQNEILLKEGDSFTLTNDDTIGDST
                     KCSVTYKDLYKDVKRGSRILINDGLIELEVENIKNRDICCIVLNGGFVSNNKGINVPD
                     ADINLPSLMPQDIEDLKFGIENDIDFIAASFVRKPSDITAIKKVLEKYGGSDIKIIAK
                     IENRQGINNFDEIIKVADGIMVARGDLGVEIPVEEVPIVQKVLIEKCYRHGKPVITAT
                     QMLDSMIRNPRPTRAEASDIANAIYDGTSAIMLSGETAIGKYPVESLEMMGKIAEEAE
                     EAMDYWKRFSSMQYDLMASVTNAISHASCTTAMDLNASAIVTVTQSGHTARMISRFRP
                     ACPVIATTTSLKVQRQLSLSWGVTPFMVKEASNTDDLFETGEAKALESGYVQNGDLVV
                     ITAGVPIGVSGTTNTMKVHIVGNVLVQGSGISMGSVTGEICVAKSLEEAKKNFKKGNI
                     LVTLSTNNDMLSILKMASGIIVEEGGINVHASIVGLALEIPVIIGASNATKILKTGSV
                     VTVDSDRGIVYYGGNRV"
     gene            12567..13067
                     /locus_tag="PK733_04875"
     CDS             12567..13067
                     /locus_tag="PK733_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOJ09910.1"
                     /translation="MDINIIPILKKEGASLNVNFSGNVNDLESRVDSYNLVDPVIFKG
                     TLQNRKGVLNLKGLIKFVYDTPCYRCLNNIHGSMEIPVKEDILNAAKVTDEDDAFTYE
                     GDYLNIDRILKDYIILNLPMKQICREDCKGLCPVCGINLNNTICSCAEEKDINPKMEI
                     LKKYFE"
     gene            13095..13280
                     /gene="rpmF"
                     /locus_tag="PK733_04880"
     CDS             13095..13280
                     /gene="rpmF"
                     /locus_tag="PK733_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOJ09911.1"
                     /translation="MANPKRKWSKARTGKRRSQWKLSAPTLVSCPQCHKLTTPHRVCM
                     ECGYYDGKDVLKKAEAK"
     gene            13426..14811
                     /locus_tag="PK733_04885"
     CDS             13426..14811
                     /locus_tag="PK733_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOJ09912.1"
                     /translation="MKEKNGTKIHDIRQGSIAEEAGLEPGDYILTINGENITDIFDYR
                     FLITDEQLDMMIKKKDGDIWELDIDKDAYEDLGIGFDDPMIDDARHCANKCIFCFIDQ
                     MPKGMRETLYFKDDDTRLSFLSGNYVTLTNVKDRDLDRIIRYRMSPINISVHTTNSDL
                     RVRMLNNKNAGDIVEKIKKLVNAGITVNCQIVLCRGINDGDELDRTISDLSSLYPWVN
                     SISVVPVGITKYREELTYLLPYDKVSASRIIEQVNLWQEKLITEKGSRVAYLADEFYI
                     MADYKIPEYEDYEDFPQLENGIGLISLFKQEFYDYLAELENKGYSIDYKVKVKKISIA
                     TGVSAYSFIKELSDVLKQRFGNIIINVFPIENRFFGEYVTVSGLLTGQDIVAQLKDKD
                     LGDELLISDSMLKAGETIFLDDYTVEMVESQLDIKLSAVKNSGKDFIEKILKIGGDEI
                     EKTYSCNSWKT"
     gene            14774..16108
                     /gene="der"
                     /locus_tag="PK733_04890"
     CDS             14774..16108
                     /gene="der"
                     /locus_tag="PK733_04890"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ09913.1"
                     /translation="MRKPIVAIVGRPNVGKSTLFNYIAGKRISIVEDTPGVTRDRIYT
                     EGEWRNRKFILIDTGGIEPFSEDEIMQQMKRQAEIAIETADVIVFMVDAKEGLTASDH
                     EVANLLRKTNKPVILAINKVDNIGEPPPEVYEFYNLAAGEFMTVSSSHGLGIGDLLDE
                     IFKHFSDEDADDDDEEIIKVSVIGKPNVGKSSLINKILGEERLIVTDIPGTTRDAIDT
                     FVEKGEDKFIFIDTAGIRRKSKIIDNIEKYSTIRSWTAVERADVCLIMIDAEEGVTEQ
                     DTKIAGYAHEQGKASIIAVNKWDLIDKETGTLEEYEKKVREKLSFMPYAPVVFISALT
                     GKRIEKLYDLIKSVAQQASIRVTTGVLNDVINEAIAMVQPPSDKGKRLKIYYITQVGT
                     KPPTFVLFINDEKLMHYSYMRYIENQLRKTFGFEGTPIKIIARERKKGDTVQ"
     gene            16105..16707
                     /gene="plsY"
                     /locus_tag="PK733_04895"
     CDS             16105..16707
                     /gene="plsY"
                     /locus_tag="PK733_04895"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOJ09914.1"
                     /translation="MSNILYYFLAAICGYLLGSINNSLVVGKFYGVDVRKHGSGNAGT
                     TNTLRTLGGKAALLVLVGDILKAIIACFIGSFLGGLTSVMIGGAFAIIGHNWPLYFGF
                     KGGKGVLTAISVIFYIDWILAVIVLGAFIVVVAITRYVSLGSVIGAAILPVLAIIFRR
                     DMEFIIFTVALAALVIIKHMPNIKRLLNGTESKLGKKVSH"
     gene            16834..17844
                     /locus_tag="PK733_04900"
     CDS             16834..17844
                     /locus_tag="PK733_04900"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOJ09915.1"
                     /translation="MGTKTISVIGAGSMGTALSILLAGNGHKVKMWSPFAEEVKMINE
                     TREQGDKLPGARIHDDTTCTCDIEEALAHSDLVVLVVPSQTIRQNAKNIKKYLKTDQI
                     VTCFSKGLEEDTGLRMSEVVAQEIPEVPYVALSGPCHAEELALGIPTAYVAASHSKKA
                     ADFAQDIFMSPKFRVYTNNDVVGVELGGALKNVIALCAGISDGLGFGDNTKAALMTRG
                     IAEITRLGIALNASAHSFAGLAGIGDLIVTCTSMHSRNRRAGILIGQGNSVQEAINKV
                     KMVVEGITTTKAAYKLAQRESIVMPITSEAYDILFNNKNPREAVDDLMTRDKTFELKG
                     IL"
     gene            complement(17827..18687)
                     /locus_tag="PK733_04905"
     CDS             complement(17827..18687)
                     /locus_tag="PK733_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09916.1"
                     /translation="MLSEYYKNPSGKINYSHSKTNKYHGMDFHLHEYFEIYFFISGKV
                     NYFIENKVYQLHYGDLIFMNNQEIHKPSVFPGEAYERIVIHFDSSIVGLLSPDDFNLL
                     DCFINRPKGEFNRINLDNEQSNHILEFLYKIGQLQKSVSAGSSIIKLTAFLDLLVYIN
                     RIYNAMQKNCDYSDIPEKLRLIMDYIDKNAEENLSLDALGKKFFIDKYYLSRLFKKYT
                     GSNIHDYILYKRISKAKRVLSEGYSATEASAVCGFNDYSNFYRMFKKTVGVSPSRYKK
                     KVNFELQNPF"
     gene            18878..19636
                     /locus_tag="PK733_04910"
     CDS             18878..19636
                     /locus_tag="PK733_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ09917.1"
                     /translation="MDKNVIAAQLYTLRDFTRTPADIENTLKKVKKIGYDAVQVSGMG
                     PIDPVQLKDIADKAGLKIVATHISPDRLKNDFNALAKEHILWDCKYIGIGSMPPEYPR
                     NKEGFKAFAKDFSKIARQCADRGLSFIYHNHKFEFEKFDGVTGMEILLNETDPEVFNF
                     EVDTYWVQAGGGEPAEWIRKVKGRMDVVHFKDMAIKENSQVYAEIGEGNLNWGSILEA
                     CKDTGVKWYCVEQDVCLRDPFESLAISLQNMKKM"
     gene            19677..20534
                     /locus_tag="PK733_04915"
     CDS             19677..20534
                     /locus_tag="PK733_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ09918.1"
                     /translation="MSKFIISAFADEIDTNLTTQMNVLEQHGIKYIEMRGVNGKSVVK
                     YTTEEMKEIKKQLDDSGFKISAVGSPIGKINIKDLFEPHLDLFKHTLELAKILETKYI
                     RMFSFFIPEGENPIKYRDEVMERWQKFIKAADGYELVLLHENEKDIYGDTPERCLDLF
                     KSMNCNYLKAIFDPANFVQCDVETFPHAYMILKDYIEYLHIKDALSSNHSVVPSGYGD
                     GRVKDILIDLFNSGYEGFLSLEPHLGSFKGFSELEPNSNINDMPEGGPKQFAIAANAL
                     KKIIDAVIK"
     gene            20554..21669
                     /locus_tag="PK733_04920"
     CDS             20554..21669
                     /locus_tag="PK733_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09919.1"
                     /translation="MEKVRIGIIGLGHMGTGHARYIFNGQIPQGVLTAVCDTDTSRLE
                     FAKREFGDNVQRFDNPDLFFTNAPVDAVIIATVHYSHPELAIKAFKHNLHVLVEKPAG
                     VYTKQVRPMNEAANSSGKVFGIMYNNRTNPVYQKVRDLITAGELGEIKRTNWVITTWY
                     RTQKYYNSGGWRGTWSGEGGGVLLNQSPHQLDLWQWVCGMPKKVRGFCHYGKYHDIEV
                     EDDVTAYVEYENGATGTFITSTGETPGTDRLEIAGDRGKIVVENNTITFWRLRESERE
                     FIRDLTKTVGSPECWKCEIPFSGSTTGHAGITANWVEAITRGTPLLAPGIEGIRGLEI
                     SNAIYLSSWIDNWVEIPVDEDLFYAKLQERIRESSQK"
     gene            22038..22442
                     /locus_tag="PK733_04925"
     CDS             22038..22442
                     /locus_tag="PK733_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ09920.1"
                     /translation="MREFQVLLYTVNGDIFGTYADEVVEIIDSKGLADGFITVRDVRV
                     PVIKLGTFLGKGDTTVSTDSKVILVNQNGHYVGFLVDSVTELIRLQENSVHNAPEILK
                     TPENRFIVKFGIAGEQPFPIIKLSYIDVERVY"
     gene            complement(22552..23787)
                     /locus_tag="PK733_04930"
     CDS             complement(22552..23787)
                     /locus_tag="PK733_04930"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOJ09921.1"
                     /translation="MKYIVILGDGMADYPVPQLGGKTPLQCAVKPNMDYIASKGISGM
                     VKTIPDGMAPGSDVANLSVMGYDPEKYYTGRSPLEAVSMGIKLGDNDKAFRCNLVTLS
                     DGDNYMDKTMVDYSSDEISSKEGAELIKEINNYFQSSEISFYPGVSYRHCMVWENAPD
                     GFKLTPPHDISGKNIASFIPEGKNSSILFKMMEDSYNILKGHKINIERLKRGLNPANS
                     IWLWGEGKKPSVPGFYEKYGIKGSVISAVDLVKGLGICMGLSSINVQGATGNLNTNFA
                     GKADAALNELEAGQDFVYIHIEAPDECGHRYEIDNKVKSIELIDEQVIGRLLAGLKNI
                     GDYRMLLLPDHPTPLAIRTHASDPVPFVMYGEGINNESCEPSYSAVSCYDEFEAKKSG
                     LFVGTGCKLMDYFISGKFE"
     gene            23965..24411
                     /locus_tag="PK733_04935"
     CDS             23965..24411
                     /locus_tag="PK733_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOJ09922.1"
                     /translation="MKKITGLFEQKLRLKGYRVTGQRQAVLDVIVNNEGRHLCSEEIY
                     ELVKADFPEIGLATVYRTLFLLENMGIIYKIDLDDGKGRYELNKGGEDHLHHHLICTE
                     CGSISEVNYDLLEFLEEQIYNKNDFLVKNHSVKFYGICGKCLKTLP"
BASE COUNT         8200 a         3830 c         5021 g         7410 t
ORIGIN      
        1 atcaccgaga tatgcagaaa agctgaaata cttgttgcag ctgcaggcaa agcaaatatg
       61 attactaaag attatgtttc accaggtaca gtagttattg atgtaggtat aaatgtggac
      121 gacaaaggta atctttgcgg agatgtgaat tttgatagcg ttgcagatat agccggaagc
      181 ataaccccgg tacccggcgg ggtagggact gtgactactt cggtcctggt aaaacacgta
      241 attaaggcag cagtagcagc tttaaaataa catggtagag tgattaagca tgggtgaata
      301 ttatttttta gtgaggagat gattttattg gttaaaaaaa tatcaaagtt tctgattgtt
      361 gctattattc ttgtaatagc tggaatatgg ctgtcatcag gattttatac agtaaaatcc
      421 ggtgaagaag ctgttgtttt aaagtttgga aagcatgttg gtactgtatc gaaagcaggt
      481 ttaaactggc atttgcctgc tccttttgaa agtgtaataa aagtaaatgt tcttgaggtt
      541 aaacgtctgg aattcggata taaaacaatt aaggaaggcg gacgcggggc agattcggat
      601 tatgcagata ttgcaccggc attaatgctc acaggagatg aaaaccttgt taatgttgaa
      661 gccgcaattc aatataaaat taaagagatt gatgattatg tgtttggtgt aaaaagtcat
      721 gatgaaactt tacaaatagc tgcagaatcg gcaataagaa gggtaattgc aaatcatatt
      781 cttgatgaag ttctcacaga gaataaatat ataattcaac aagaaataaa agaggactta
      841 cagaagacat gtaatgatta taaacttggt ataggaataa tagcagtgca gcttcaggat
      901 gtcaatcccc ctgacgaagt tgatgcggct tttaaagatg tagctaatgc aagagaggac
      961 agaaacagct atattaatga agcagaaagc tacaaaaatg aagttattcc aaaagctagg
     1021 ggtaatgcag ctaaaatggt aaacgatgct ttagcttaca aagaaaaaag aatagcagag
     1081 gcaaaaggtg atgttgccaa ttttgcccaa atacttgaaa aatatgaaca gggaaaggat
     1141 gttaccagga ttcgtatgta tcttgaaaca atggaggaag ttcttcccgg aattgataaa
     1201 tatattgttg attcacaggg caacttaatt aaattccttc ctcttgagca aagtcaaact
     1261 cttcaaaatg gttctaaaca ggagaaagga gggtccaact aatatgaacg ataacatggg
     1321 taatatgggt aatagcaaca atatgggatt tggacagcaa agatataatt ttaatctgtc
     1381 aaactttaaa ggcttttata aaattgcagg tattactatc atagctgtta ttcttttaac
     1441 cgtaatatcc aatatgtttt tcttttctgt agatgaaaga gaacaggttg tgatcaaaca
     1501 atttaatgag gttgtaaaaa tagtagttga tgaaaatgta gataagattc gagagatagc
     1561 cagcgaaaac ccacagttaa gggatataaa aattgacaat agaaaaggct tgcatttcaa
     1621 agtgcctttt ctccaaactg tagaatcttt tacaagcatg cttttgacat atgataccga
     1681 agtaagggaa gttattacaa aagacaaaaa gaaactggta ctggataatt ttgctgtgtg
     1741 gaagatcacc aatcctgcac ttttttctat ttcacttaga aatgaaaggg ctgcccatac
     1801 aaggcttgac gatataatat actccaagct gaatgaggaa ataggaaaag ttgaggctca
     1861 tgtagtaata tctgataaaa attttgttga agaaatgctg gatagggtta tacaaagcac
     1921 caataaacag gtggagagtt atggtatgga agttgtggat gtcaagataa aaaagactga
     1981 tttacctgta gcaaactaca gtaacatttt caaccgtatg aaaacagaac gtgaaagatc
     2041 agctaaaacc taccgttctg aagggcagga agaagctcag aaaatccgtt ccgaagctga
     2101 taaggaagca acaattattg aagcaaaggc ctatgaagaa gctgagaaaa caaaaggtga
     2161 aggtgatgcg gaagcattaa gaatttatgc agaagcatac aataaagatc ctgaattcta
     2221 tgccttttgg aggtcactac aggcctataa gaaaacttta aaagacaata ccaagataat
     2281 tatcgactca aattcggaat ttgcaaagta tttgtataat ataaagtgag ccaaaccaat
     2341 ctgagcagtg atagttgggg aatatacaag ataattcaag gtagtgggga cggttaacaa
     2401 tttgagaacc gtcccctaaa caaaccgcct cctaaacaag catccgattc acttgctgct
     2461 tttttctttg ccttgacctg atgattggct tgacgccttt tcaaggtttt tatccacttc
     2521 ctcaatattg gataaggtta ttactccttt gatatctgtt aagggttgat ttttttcttt
     2581 tataaccttt tctaattctg taattgaaaa gtccagctta ctggcagcct tcttattttc
     2641 ttcaccaagc atgtttttta gaaaacccca tactgatttt aaattgttaa tatttgtttc
     2701 agcctgcgtc caatcactat tcatagcact gagtactacg ttccttgtaa agtattttaa
     2761 cctctttact tcactgacat tttgcttttt atagagagag aacaaacctg taatatttcc
     2821 ataaaggtta tttgcactta ttagtgtgta attcttgttt tttgaaatga tggaatttgt
     2881 cagaccattc aaggaactgc tgaaaccgtt tataatttcc tgacttgccc ctttttgtac
     2941 tgcttcaggc atgtagctgt tccaggtaaa gtgcatttta ttgataatag gggttacctc
     3001 ttgccaggga tctttttcct gaggtttctt agctccggtt tccgtctgct gtgtttgctg
     3061 ttgcttcccc tgtccttctt gtccttgctg accttgctgc tttccctgat ctgattgttt
     3121 gtcttcggat ttggctccgt cctccttttc agtatcttgc ttttttgtct ggtcactact
     3181 gccttgtttt tcttcctcag cacctttttt accgctttct tccttctcag tttccggggc
     3241 agaaggacca ttaagagctt ttattatttt ttctatacct tgttccattt ctgtcaattc
     3301 accagggatt ttgccagatt caccctctga ctctcctgat tggcgttggg cggaaggtcc
     3361 tttttcttca cgattgcagc ccgatatcaa tatagtaatt gcaaatgcca taattaaagc
     3421 gaatgctatc atgcggttta gtagtttatt ttttatgctt atatttgcat tgtctccatt
     3481 ttccttatct ctatttttat tattgattaa catatttata actgcaaacc ctccatttcc
     3541 atatttctat atagaacagg ttttaaaaac atttaaaacc tgcttctgtt accttgatgt
     3601 aatttacgat ttacaaactc ttcattagtt ctttcaaatc gtaaattata taataaacat
     3661 ctcagtttta gtaatgagta attacagtga ataatattct aaaattaaga gaaaatattt
     3721 taattttgga tgtagggtgt atattttatt tttgaagaaa ttattggaag aagcattaat
     3781 gaaggctcta tgtaccgtaa cgatagcttc tggaatatat ttcttcaaaa attctaaatg
     3841 cacccttaga tgcacttttt tattgtggca tattgtgtta aaatatagta tgtgtttaaa
     3901 aggaggggtt tgataatgag tgcctatgat attatattaa gaagaaggac cattagaaag
     3961 tttaagcagg ataaaatcga gagggaatta ttggtaaaac tggtgaatgc tgcacgacat
     4021 gcaccctccg gtgcaaatat gcagcctgca aaatatttaa ttatagatga cagtaatact
     4081 gtgaaaaaaa tatttgaaca tttaaaatgg gctggataca tagctccaca cggtgatccg
     4141 aaggaagggg aaagacctgt agcatatatt atagttctgg ctgatacccg gatcagaaag
     4201 agcggatatg agctggacct gggtgcagca atacaaaata ttcttatcac agcagaggag
     4261 gaaaatatag gcacttgctg gataggatca gttaatcgtg aggaagtaag gcaaatactc
     4321 gatattccag atgaactgtt aataaatagt gtgatagctc tgggctacaa ggcagagagt
     4381 tctgttgcag aggaagaaaa tggttcaata aaatattata aggataataa aggtatattg
     4441 catgtaccaa agaggaaact taaggatata atggaggtga gcagatgagc ttttcaaaag
     4501 aaaccataga ctatattgcg gagctttcca gaataggtct ttcagaaaaa gagaaagaag
     4561 atatggttgc tcctgtagaa aaaatattag catgcatgga caagcttgag gaactggata
     4621 caacaaatat tgaaccaatg gagcatattg tgtctgctgt aaatgtattc agagaagata
     4681 aagtggaaaa atcttatgat agggatatta tacttgctaa tgctcctgaa catgaagatg
     4741 gttgttacag tgtccctaaa attgtggagt gaatggagga aaatttgtga aactgtatga
     4801 acttactgta agccagttaa gcagtttact aaagaaaaaa gaaataaatg ctgtagagct
     4861 taccagaact gttttagata ggattgtaga agcagaacct gtattagatt gttatattaa
     4921 catcattgag gagcaggcag ttgaaagggc tgaaaaggtc caaaaaatga tggatgctgg
     4981 tgaattgtat tcaccgttgg caggtatacc tatggctgtt aaagataaca tttgtaccga
     5041 aggcatacct actacctgtg catctaaaat gctgcaagat ttcgttccac cttataacgc
     5101 tgcagttatg cagaaattgt ataataatgg ggcaatcctt ttaggaaaac ttaacatgga
     5161 tgaatttgct atggggagtt caactgaaaa ttcctatttt aagaaaacta aaaatccatg
     5221 gaatcatgat agagtaccgg gaggatccag tggaggatgt gctgcggctg tagctgcagg
     5281 agaagctata tattctctcg gcacagatac aggtggatct ttaaggcagc catcagcctt
     5341 atgcggggtt actggtttta aacccacata tggttcagta tcaaggtttg gacttatagg
     5401 atatgcttca tccttcgatc aaattggtcc ggttgcaaaa gatgtaaccg atagtgctct
     5461 tgtacttaat gctattgcag gtcatgattc tcaggactct acatcagccg gtatcaagta
     5521 tcctgattat acggaatcac ttgttaataa tgttaaaaat atgaaaatag gcataccatc
     5581 agagtatatg aggaataaca caagtgagga tgtaagaaag gctgtttcgg aagctattga
     5641 gttatttgaa aagcttggag ccaaatgcga agaattttct ctacctataa tggagtatgt
     5701 actgccaact tactgcataa ttacatttgc tgaagccagt tcaaatctgg gcaggtatga
     5761 tggtgtaaaa tatggccaca gagcaaagga ttacaaggat ttgggagaac tgtacaaacg
     5821 tacaagggat gagggcttcg ggtcagaagt taaacggaga attattcttg gaacctatgt
     5881 attgagtgca ggacaatatg ataaatatta taagaaggct ttacaggcaa gaagggttat
     5941 atgccatgag cttaataggg cttttgataa atatgatctg attatgggtc ctacatcacc
     6001 ttcaactgcc ttcaggtttg gtactaaggt tgaagaccct gcggaaatgt acaaaaatga
     6061 tgttttaact atgccggcaa acattgcagg acttcctgca atatctgttc catgcggttt
     6121 cgatggcgac agcatgccaa taggcttgca atttataggt aaacagtttg gggaaagtac
     6181 tttattaaga gcagcatata catttgaaca aaatacggat tttcataaaa ataaaccagt
     6241 tattaaacca gttatttgat ttgaggtgag acaatgaatt acgaagtagt aataggatta
     6301 gaagtacatg cagagctttc caccaactct aaaatatttt gttcctgtac aacagagttt
     6361 gggggtgacc caaatactca tacctgccct ggttgttcag gtatgccggg gtcattaccg
     6421 gtgttaaaca gaagagttgt agaatatgca ataaaagccg gacttgctac aaattgcatt
     6481 atttcaaatt atacaaaaat ggacaggaaa aactattttt accctgatct acctggcgct
     6541 tatcagattt cacagtatga tatacccata tgtaaaaatg ggtatataga aattgaagtc
     6601 gaaggaaaga aaaagagaat aagggttaca agaattcata tggaagaaga tgcaggaaaa
     6661 cttatgcatg atgaatttgg tgcaggatcc ctgatagatt ataatcgggc gggtatgccc
     6721 ttagttgaaa ttgttacgga acctgacata agatctgcag aagaggcaaa agcatttctg
     6781 gacaatctga agtcaatctt gcagtttatt gatgtatcgg actgtaaaat ggaggagggt
     6841 tctttaagat gtgatgtaaa tatttccgta aggcctgaag gtgaagaaaa gcttggtgta
     6901 agaacggaaa tgaaaaattt aaactccttc aaagcagtta ttagtgcaat agaatatgaa
     6961 tcaaaaaggc atattgatga gattgaatca ggaggggtaa tttacaggga aacaagacgc
     7021 tgggatgatg ataaaggctt tagctattcc atgagaagca aggaacaagc tgaagattac
     7081 aggtattttc ctgacccgga tcttccgcca ataattattg accaggagtg gattgagaaa
     7141 atacggagca ctcttccgga attgcctgaa gccagaaagc aaagatatat gaaggagtat
     7201 gatttaccgg cttatgatgc aggtatactt acatcttcaa aagtaatatc caactttttt
     7261 gaaaaggcac tgagaaaaac tgataatgtg aaagccgtca gcaactggat tatgggtgac
     7321 ttgatgcgca tattgaaaga cagaaaatta gaagtggaaa atataccatt ccctgctgaa
     7381 tatttggcag atatgatttc actcatagat aaagggacta tcagcgggac tatggccaaa
     7441 aaggtatttg aaaaaatgtt tgattcagga aaggctcctg aagttatagt gaaagaagaa
     7501 gggttggagg ttgtaactga tgaagctgct attgcaaaaa tagttgataa ggtattggat
     7561 aataaccccg gatcagttgc agattataaa gccggaaaga aaaaagcctt gggatttctt
     7621 gtaggacaag ttatgaagga atcaaaagga aaagctgaac cgcagcttgt aaacagactc
     7681 ctttcagaaa aactgaatga atagggaata agagtgaatg aatagggaat aagagtgaat
     7741 gaatagggaa taagagtgaa tgaataggga ataagagtga ataataggta aaatagtata
     7801 atatttattt ggtgcattta atttacttga agaaatattt ctttagattt agttaatgca
     7861 ccagtatttt ttttaaagta gccttgtata caataaacgt tttgtatata ataaatatgt
     7921 tcggaaagcc ttgataagac tagatattag cacctggtaa taatgaaagt aaaggttgta
     7981 acgacgttgg attatgtaga atttaggtgt tcaatacacc tctaaagcga aaggagtatg
     8041 atataatgaa ctttcttgaa agaaccattg aaagggcaaa atcagacatt aagacaatag
     8101 ttttgcccga gagtaccgac atacgtgtaa taaaagcagc atcaattgct ttggaaaagg
     8161 gtgtagctaa tattgttctt gtaggcaacc gcgaggaaat tttaagcaaa gcagggtctt
     8221 tggatatttc taaagctgta atagtagatc ctttgaagtc tgaaaagtat gatgattatg
     8281 ttaacacttt ttatgaacta agaaaagcaa aaggaattac tgttgaaaaa gcacaggaaa
     8341 ttatgaaaga ccctgtttat tgggctgtaa tgatggttaa aaagggagat gctcatggaa
     8401 tggtgtcagg tgcggtaaat tcaacagcca atacattaag acctgcattg cagatagtaa
     8461 aaaccgcgcc ggatgcaaaa cttgtttctg ctttcttcct gatggttgta cctgactgtg
     8521 aatatggtca taatggtaca tttatttatg cagacagtgg tttggttgaa aatccaaatg
     8581 aagcggaact ggcagaaata gccataacgt cagctaaatc atttaagctt cttgctgagg
     8641 ctgagcccgt agttgcaatg ctttcatatt ctacatatgg aagtgccaaa agtgaactga
     8701 ctgaaaaggt tattaaagca acaaagcttg ctaaagaaaa ggcaccgaat ctgcttcttg
     8761 atggagaatt acaagcggat gcagcgttag tatcatctgt tggaaaatct aaagctccgg
     8821 gaagccctgt tgctggtaaa gcaaatgtac ttgtattccc ggatctgaat tgcggcaata
     8881 ttgcatataa attaacccag cgcctggcaa aggctgaagc ttatggtcca attacgcagg
     8941 gcttggctgc tcctgtaaat gacctttcaa ggggttgttg tgcagaagat attgtgggag
     9001 ttatcgctat aactgctgtt caagctcaga aggaatgata ttccacatta tagcaaatta
     9061 gtgtttgttc aataaattat atgctaatta aaatatttat taatttattt gcttatttac
     9121 ttatgttaga aaatatttaa agttaagaat gggggtaaaa tactatgaag aaagtcctgg
     9181 taattaattc aggaagttcc tcattaaaat tccagctaat agatatgtca aatgaagcag
     9241 ttcttgcaaa aggactttgt gacagaatag ggatagagaa ttccttcttg aagtatacaa
     9301 aatatggaga ggatcaggtt attataaaca aagatatggc cgaccataaa gcagctgttc
     9361 aggaagcaat tcatgctctg gttgacaaaa aaacaggtgt aatttcggat atgtctgaaa
     9421 tatctgcagt agggcacaga gtagtgcatg gaggagaagc attccatgaa tctgtagtta
     9481 ttgatgatga agtaatgaac gctatcgaag aatgcataaa tctggctcca cttcataatc
     9541 ctccaaatat aacaggaata aaggcctgcc aacagattat gcctggtaca ccaatggttg
     9601 ctgtttttga tacggcattc catcaatcaa tacctaagta tgcatatttg tatgcacttc
     9661 cttacgacat ttatgaaaag tatggagtac gcaaatatgg tttccacgga acatcacata
     9721 aatatgtatc tcaaagggca gcagcaatac ttaataagcc tcttgaggaa cttaaattaa
     9781 tatcatgcca cttgggaaac ggagcaagca tttgtgcaat taaaaatgga aaatccgtag
     9841 acacaagcat gggatttact cctcttgcgg gtttggctat gggcacaaga agcgggacta
     9901 tcgaccctgc agtaatatca tttttaatgg aaaaagaaga tatgaccata gatgagataa
     9961 acgagtacct taataaaaaa tccggagtcc ttggtatatc aggagtaagc agtgatttca
    10021 gagatttgga agctgcagcg gatgcaggaa atgaaagagc aaagctggct attgatattt
    10081 tctgttacag agtgaaaaag tatattggtg aatattcagc tgttacagaa ggtattgatg
    10141 cagtaatatt tacagcaggt ataggtgaaa ataattataa ggtaagagag caaatattag
    10201 aaggtatgga ttatttgggc ttgaaagttg atcatgagaa gaacaaaatc agaggtaaag
    10261 agattgatgt aagtgctgat gatgcaaaag ttagaacact tgtaatacct actaatgagg
    10321 aattggctat tgcaagagaa acaatgagtc ttttaaagta gtgtaaatag gtgcatttat
    10381 caaatatgaa gaaaatattt tcaggggggg ctattgtaca gtgcttagag catccactta
    10441 tgccccttgc caatattttc ttcatataaa ttaaatgcag cttaggggtg ggaaagttag
    10501 aaatggtaag tagataactt ctcacttccc atctccaatc tataaattat cacttccagc
    10561 ctccaactgt atatttacta caaaatagat ctagaccctg ttacctccat aataaacaat
    10621 gcctctatct gagtctacag taaccactga accggttttc aatattttcg tagcatttga
    10681 tgcacctata ataaccggaa tttccagtgc taatccaaca atagaagcat ggacatttat
    10741 accgccttct tccacgataa tgcctgaagc catttttaat atagaaagca tgtcattatt
    10801 tgtagacaga gttacgagaa tgttaccttt cttaaaattt ttctttgcct cttctaagct
    10861 tttagcaaca catatttcac ctgtaaccga tcccatactt attccagaac cttgtacaag
    10921 gacatttcct acaatatgaa ctttcattgt atttgttgta ccacttacgc ctataggtac
    10981 acctgcagta ataacaacca ggtccccatt ttgcacataa cctgactcaa gagcttttgc
    11041 ttctcctgtt tcaaacagat catcagtgtt agaagcttct tttaccataa atggagtaac
    11101 tccccatgat aatgacaatt gacgttgaac cttcaggctt gtagtagtag ctattacagg
    11161 gcatgccggc ctgaacctgg aaatcatcct tgcagtatgc ccggattgtg ttacagtaac
    11221 tattgccgat gcattcaaat ccatggcagt agtgcaggag gcatggctta ttgcatttgt
    11281 tacacttgcc attaaatcat attgcatact tgaaaaccgt ttccagtaat ccatggcttc
    11341 ttcagcttct tcagcgattt ttcccatcat ctcaagactt tcaacaggat atttgcctat
    11401 ggcggtttcg cctgaaagca ttattgcact tgtaccatca taaattgcat tagcaatgtc
    11461 acttgcttca gctcttgtag gccttggatt tctaatcata gaatccagca tttgggtagc
    11521 agttattact ggtttcccat gtctgtagca tttttcaatt agaacttttt gcactatagg
    11581 tacttcctca acaggaattt caactccaag atcacctctt gccaccatta ttccatctgc
    11641 aacctttata atctcatcaa aattgtttat tccctggcgg ttttcaatct tggctataat
    11701 ctttatgtcg ctgccgccgt atttctcaag aaccttcttt atagcagtta tatctgatgg
    11761 ctttctgaca aaggaagctg caataaaatc aatatcattt tctattccaa attttaaatc
    11821 ttcaatatcc tgtggcatca gtgagggaag attaatatct gcatccggta cattaatccc
    11881 tttgttattg cttacaaatc cgccatttag tacaatgcag caaatatccc tgttctttat
    11941 attttccact tccagttcaa taagaccatc attgattaaa atgcgacttc ctctttttac
    12001 gtctttgtat agatctttat aagttacgga gcatttggta ctatcgccaa tagtgtcatc
    12061 atttgtaagt gtaaagctgt ctccttcttt taaaagtatt tcattttgtg aaaactttcc
    12121 tatacgtatt tcgggccctt ttgtatctag taaaagagct accggcaagc ctgaattatc
    12181 tctcactttt ttaaaccttt caacccgctg cttatgctct tcgcgagttc catgggaaaa
    12241 attcatacgg gcaacgttca ttccttttaa cataagctca cttaaaatat tttcatcgtc
    12301 aactgcgggg cctaaggtac atataatttt tgtccttctc atataactta cctctcaaca
    12361 ttattattta ttatattatt atttattttt atatatctga agtgctcagt tgcacaaaga
    12421 catacgaaaa agcaaaattt ggttacttat aattataaca tggtactatt tttttgtaca
    12481 agtttaaaat tccaggctta aaaatccttg acaatgctaa tattagttag tataattact
    12541 tttgtcggtt tcgagaggtg gcaggtatgg atataaatat aataccaatt ttaaaaaaag
    12601 aaggcgccag cctgaatgtt aattttagcg gaaatgttaa tgaccttgaa agtagagtag
    12661 atagttacaa tttagttgat cctgtaatct ttaaaggaac attgcaaaac cgtaaaggag
    12721 tattaaatct aaaaggttta ataaaatttg tgtatgacac accctgctac aggtgcctga
    12781 ataatattca tggaagtatg gaaatccctg taaaagagga catactcaat gcggcaaaag
    12841 ttaccgatga agatgatgca ttcacctatg aaggtgacta tcttaatata gatagaattt
    12901 taaaggatta tataatcttg aatttgccga tgaaacagat ttgcagagaa gactgcaagg
    12961 gattgtgtcc tgtttgcgga atcaacctga acaatactat ttgtagttgt gcagaagaaa
    13021 aagatattaa tcccaaaatg gagatactga aaaaatattt tgaatagaag atatgtaaga
    13081 tggaggtgtt acaagtggca aatccaaaac gtaaatggtc caaggcaagg acaggtaaaa
    13141 gaagatcaca atggaaacta tcagccccaa ctcttgttag ctgtcctcaa tgtcataaat
    13201 tgactacacc acatagagtt tgtatggaat gcggttatta tgatggtaaa gatgtgttaa
    13261 agaaggctga ggccaaatag aatcatatga gtgtgtatct gatgaaaaag agcagcagtg
    13321 gaaataactg ctgctctttc actagaggtg catacctgca cctaaactca gcatgactca
    13381 atgtcgtgct gagtttgact aatatcccgg aggcggaaac agcaggtgaa agagaaaaat
    13441 ggtactaaaa tacatgatat acgacagggt agtattgctg aagaagcagg attagaacct
    13501 ggagattata ttttaacaat aaacggtgag aatattaccg atatttttga ttataggttc
    13561 ctaattactg atgagcagct tgatatgatg attaagaaaa aagatgggga tatttgggag
    13621 ctggatattg ataaggatgc ttatgaagat ttgggaatag ggtttgatga cccaatgata
    13681 gatgatgcca gacactgtgc aaataaatgc atcttttgct tcatagacca aatgccaaag
    13741 ggtatgagag aaactcttta ctttaaagat gatgatacaa ggctctcttt tttatcaggt
    13801 aactatgtaa cgctcactaa tgtgaaggat agggatttgg acaggattat aaggtaccgc
    13861 atgtcaccga taaatatttc tgtccatacc acaaattctg acctgagagt tcgtatgctt
    13921 aataataaaa atgccggcga tattgtagaa aagataaaga aactggttaa tgcaggaata
    13981 acagtgaact gccaaattgt attgtgcaga ggaataaatg atggtgatga actggacagg
    14041 acaatttctg atctttcgag tttatatccc tgggttaata gtatatccgt agtaccagtt
    14101 ggaataacca aatacaggga agaactaaca tatttattac catatgataa ggtatcagct
    14161 tcccggataa ttgagcaggt aaacctatgg caagaaaagc tgattactga aaagggttca
    14221 agggttgctt acctggcaga tgaattttat attatggcag actataaaat accggaatat
    14281 gaggactatg aagacttccc tcaacttgaa aatggtattg gacttatttc attattcaaa
    14341 caggaatttt atgattatct tgctgaactt gaaaataaag gttatagtat tgactataag
    14401 gtcaaagtca agaaaattag catagcaaca ggtgtatcag cctatagttt tataaaggaa
    14461 ctatcagatg tgctgaaaca aaggtttggt aatattatca taaatgtgtt tcctatagaa
    14521 aacaggtttt ttggggaata tgtaactgta tcaggtcttt taacagggca ggatattgtt
    14581 gcacaattga aagataaaga tttgggagat gagttattaa tctccgattc aatgcttaag
    14641 gcaggagaga cgatttttct agatgattat acagttgaga tggtggaaag tcagttggat
    14701 ataaagttga gtgctgtaaa aaatagtggt aaggatttta ttgaaaaaat tcttaaaatt
    14761 ggaggtgatg aaattgagaa aacctatagt tgcaatagtt ggaagaccta atgttggcaa
    14821 atccacctta tttaactata tcgccggtaa aagaatctca atagtagagg atacacctgg
    14881 agtaacccgg gacaggatat atacagaagg tgaatggagg aatagaaagt ttatccttat
    14941 tgatacagga ggaattgagc ctttctctga agatgaaatt atgcagcaga tgaagagaca
    15001 ggcagaaatt gctattgaaa ctgcggatgt tatcgtattc atggtggatg caaaagaagg
    15061 attaacagct tcagatcacg aagtagccaa cttgcttcgc aaaaccaaca aacctgttat
    15121 tttggctata aacaaagtcg ataacattgg tgaaccgcca cctgaggtat atgaatttta
    15181 taatcttgca gcaggtgaat ttatgactgt ttcatcttct catggtttag ggataggtga
    15241 tttgcttgat gaaattttta aacatttttc cgacgaagat gcagatgatg acgatgagga
    15301 gattattaaa gtatccgtta taggtaaacc caatgtaggc aaatcttcat taattaacaa
    15361 aattctaggt gaagaaaggc ttattgtaac tgatataccg ggcacaacca gggatgcaat
    15421 cgatacgttt gttgaaaaag gtgaagataa gtttattttt atagatactg caggtattag
    15481 gagaaagagc aaaattatag ataatataga aaagtacagc acaatcaggt catggacggc
    15541 tgtagaaaga gcggatgttt gcctgattat gatagatgct gaagaagggg ttaccgagca
    15601 ggatactaaa atagccggat atgctcacga gcagggaaaa gcatcaatta ttgcagtaaa
    15661 caaatgggat ttgattgata aagaaactgg tacactggaa gaatacgaaa agaaggtacg
    15721 tgagaagtta agttttatgc catatgcccc tgtggttttt atatcagcct taacaggtaa
    15781 gagaattgaa aagctgtatg atttgatcaa gagtgttgca cagcaagctt ctataagagt
    15841 aacaactggt gttttgaatg atgtaataaa tgaagccata gcaatggtac aaccaccttc
    15901 tgataaaggc aaaagactta aaatatacta tattactcag gtaggaacaa aacctccaac
    15961 gtttgtactt tttattaatg atgaaaaact tatgcattat tcgtatatga gatatattga
    16021 aaatcagcta agaaaaacct tcgggtttga aggtacaccg ataaaaataa ttgcaagaga
    16081 aagaaaaaaa ggggatactg tgcaatgagt aatattttat actatttttt agctgcaatt
    16141 tgcggttatt tgcttggcag tataaataat tctctggtag taggtaaatt ctacggtgtg
    16201 gacgtaagaa agcatggcag cgggaatgca gggacaacca ataccctgag aacccttggg
    16261 ggtaaagccg ctttacttgt actagttgga gacatactaa aagctataat cgcctgcttt
    16321 ataggaagct ttctcggtgg cttaaccagt gttatgattg gcggggcttt tgcaataatc
    16381 ggtcataact ggccgctgta ttttgggttt aaaggtggaa agggtgtgtt aacagctatt
    16441 tcggtaattt tttacataga ctggattttg gctgtgattg tgctgggagc atttatcgtc
    16501 gtagtagcaa taacaagata tgtatccctg ggatctgtta taggtgcggc tattttgcct
    16561 gtattagcta taatatttag aagggacatg gaatttataa tatttactgt tgctctggca
    16621 gctcttgtaa ttattaagca tatgccgaat attaaaagac tgttaaatgg cactgaatca
    16681 aagttaggta aaaaagtaag tcattaatga atattgggtg catttaaaat tttcaaagaa
    16741 atatatttca gaagctatta tttaatttct tcaaaaataa aatatgcacc ttgatgaatg
    16801 aaaaagtaaa atacattaat ttagggtgat atcatgggaa ctaaaactat atccgttatt
    16861 ggtgccggaa gtatgggtac ggcactttct attttattgg cagggaatgg acacaaggtt
    16921 aaaatgtggt ccccgtttgc agaagaagtt aaaatgataa atgaaacaag agagcaagga
    16981 gataaacttc caggtgcaag aatacatgat gatactactt gcacctgcga tattgaagaa
    17041 gctttggcgc attctgatct tgtagtactt gttgtacctt ctcaaacaat aagacaaaat
    17101 gctaaaaaca ttaaaaaata tttgaaaacc gatcaaatag ttacctgttt ttccaaaggt
    17161 cttgaagaag atactggact tagaatgtct gaggttgttg cacaagaaat acccgaagtt
    17221 ccatatgtag ccttatcggg gccctgtcat gcggaagaac tggcactggg tatacctaca
    17281 gcatatgtgg ctgcatcaca ttcaaagaaa gctgcagatt ttgctcagga tatatttatg
    17341 tctcctaaat tcagagttta tacaaataat gatgtagtag gtgtagagct tgggggagct
    17401 ttgaagaatg ttatagcttt gtgtgccgga atatctgatg gattgggctt tggagataat
    17461 accaaagcag ccttgatgac cagaggaatt gcagaaataa ccaggctggg tattgcactt
    17521 aatgccagtg ctcatagttt tgcaggcttg gcaggtattg gtgaccttat agtaacctgc
    17581 acaagcatgc acagcagaaa tagaagagca ggaatactga ttggccaggg gaattcagta
    17641 caggaagcca ttaataaggt aaaaatggtt gtagaaggta taactactac aaaagctgca
    17701 tataagcttg cgcaacgaga aagtatagtt atgcccataa cctccgaagc ctatgacatt
    17761 ttatttaata acaagaatcc cagagaagct gttgatgatt tgatgaccag ggataaaact
    17821 tttgaactaa aagggatttt gtaattcaaa gtttaccttt tttttataac gtgaggggga
    17881 tacaccaact gtttttttaa acatcctgta aaaattcgaa taatcattaa acccgcaaac
    17941 agcagatgct tcagttgcac tgtatccctc agaaagcact cttttagctt ttgatatcct
    18001 tttatataat atataatcat gtatattgct tcctgtatat tttttaaaaa gtctgctcag
    18061 gtaatatttg tctataaaaa actttttccc aagagcgtca agggataaat tttcttcggc
    18121 atttttatca atataatcca ttattaatct taacttttcc ggaatatcac tgtaatcaca
    18181 gtttttctgc attgcattat atattctatt aatatagaca agaagatcca gaaaggcagt
    18241 tagtttgatt atactggaac ctgcagaaac acttttttgt aattgaccga tcttatatag
    18301 aaactcaaga atgtggtttg actgttcatt atccaggttt attctgttaa attcaccttt
    18361 tggcctgtta ataaagcaat cgagtagatt aaaatcatca ggacttaata aacctactat
    18421 tgatgaatca aaatgaataa ctatcctttc ataggcttca cctggaaaca cagatggctt
    18481 atgaatttct tgattattca taaaaattaa atctccgtaa tgtaattggt atactttatt
    18541 ttctataaaa tagtttactt tacctgaaat aaaaaagtat atttcaaaat attcatgaag
    18601 atgaaaatcc atcccatgat acttattagt tttagaatgg gaataattta ttttacccga
    18661 aggattttta taatattctg aaagcatagt cataaacctc cattttggtg cctgttgtat
    18721 ttggtgcccg gcaccaaata caacaggcac caaaagacaa ctaaaacaat ataatagcca
    18781 ataaaagcaa agattatgct ttaattatat aatataatag caagtatagt atatacttta
    18841 ataaaatgtt ataaataatt tttacaggag ggaaaaaatg gacaagaatg taatcgctgc
    18901 tcaactatat actctgaggg attttaccag gactccagca gatattgaaa atactttaaa
    18961 aaaagttaaa aaaataggtt atgatgcggt gcaggtatca ggtatgggac ctatagatcc
    19021 tgttcaattg aaagacattg ctgataaagc aggcctgaaa atagttgcaa cccatatatc
    19081 tcctgatcgg cttaaaaatg attttaatgc tctggctaag gaacacatat tatgggattg
    19141 taagtatatt ggtataggca gtatgcctcc cgaatatcca agaaataaag aagggtttaa
    19201 agcatttgca aaggattttt ctaaaatagc aagacaatgt gcggatagag gattgagttt
    19261 tatataccac aaccataagt tcgaatttga aaaatttgat ggtgtaacag gtatggaaat
    19321 cctattaaat gaaacagacc ctgaagtatt taattttgag gttgatactt actgggtgca
    19381 ggccggaggc ggagaacctg cagagtggat taggaaagta aaagggagaa tggatgtagt
    19441 gcattttaaa gatatggcaa taaaggaaaa tagtcaggtt tatgccgaga ttggtgaagg
    19501 aaatctcaac tggggttcta ttctagaagc atgtaaggat actggtgtta aatggtactg
    19561 tgttgagcag gatgtatgcc tccgtgatcc atttgaaagt ctggcaataa gtcttcaaaa
    19621 tatgaagaaa atgtgacata tgcctgtgta ttttaatata aaaggaggct gtttccatga
    19681 gcaaatttat tattagtgct tttgcagatg aaattgatac taatttaaca acacaaatga
    19741 atgtacttga acagcatggg atcaaataca tcgagatgag aggtgtaaac ggtaaaagtg
    19801 ttgttaagta cactactgaa gaaatgaaag aaataaagaa gcagctcgat gatagtggat
    19861 ttaaaatatc agctgttggc tcacccatag ggaaaataaa cattaaggat ttatttgaac
    19921 cgcatttgga tctttttaaa catacattgg aattggcaaa aatattggaa accaaatata
    19981 tcaggatgtt tagttttttt attcccgaag gagaaaaccc gataaaatac agggatgaag
    20041 taatggaaag atggcagaaa tttatcaaag cagctgatgg gtatgagctt gtacttttgc
    20101 atgaaaatga aaaggatata tatggcgata cccccgaaag atgtcttgat ctttttaaga
    20161 gtatgaattg caattatttg aaagccatat ttgacccggc caatttcgtc cagtgtgatg
    20221 ttgaaacttt cccccatgca tatatgatac taaaagacta tatagagtat ttacatatta
    20281 aggatgcgtt atcaagcaat cacagtgtag taccttcagg atatggagat ggcagagtta
    20341 aagatattct tatagattta tttaatagtg gttacgaagg cttcctatct ttagaacccc
    20401 atctaggttc tttcaagggc ttttcggagc ttgaacccaa ctcaaatatc aatgatatgc
    20461 ctgaaggagg tccaaagcaa tttgccattg ctgctaatgc attaaagaaa ataattgatg
    20521 cagtaataaa ataatataaa ggtggagaaa tgtatggaaa aggtaagaat aggtataatt
    20581 ggtttaggcc atatgggaac aggccatgcc aggtatatat ttaatggtca gatacctcaa
    20641 ggtgtcctta cagcagtgtg tgacacagat acatcacgac ttgaatttgc aaaaagagaa
    20701 ttcggggata atgtgcaacg gtttgataat ccggatttat tttttacaaa tgcacctgtt
    20761 gatgcagtta taattgctac agtacattac tcgcatccgg aacttgcaat taaagcgttt
    20821 aaacataatc tgcatgtact tgttgagaaa ccagcaggtg tttatacaaa acaggtacgt
    20881 cctatgaatg aagctgcaaa tagcagtggg aaagttttcg gtataatgta taacaacaga
    20941 accaatcctg tataccaaaa agtaagggat ttgattacag caggtgagtt aggagaaatt
    21001 aaaagaacga actgggttat tactacatgg taccgaacac aaaaatatta taactcagga
    21061 ggttggagag gtacatggtc aggtgaggga ggtggcgtgc tcctaaatca gtcccctcat
    21121 cagttggatc tttggcagtg ggtatgtggt atgcccaaaa aggtgagagg tttttgccac
    21181 tatgggaaat atcatgatat tgaagttgaa gatgatgtta ctgcctatgt tgaatatgaa
    21241 aatggagcta caggtacctt tataacatca acaggtgaaa ctcctggaac agaccgtctt
    21301 gaaattgctg gagacagggg gaaaattgtt gttgaaaaca acactattac attctggcgt
    21361 ttaagggaat cagaaaggga atttattaga gatttaacta aaaccgtagg ttcgcctgaa
    21421 tgttggaaat gtgaaatacc gttcagcggg tccactacag gacatgcagg tattaccgca
    21481 aattgggttg aagcaattac cagaggaaca cctttactag caccaggtat tgagggtata
    21541 aggggccttg aaatatcaaa tgcaatttat ctgtcttcct ggattgacaa ttgggttgaa
    21601 attccggtag atgaagacct tttttatgct aaacttcagg aaaggataag agagtcaagc
    21661 caaaaataaa ttgtatgtac ctcctattaa ttggtgcatt tatcaaatct gaagaaatat
    21721 ttaccagtgg ctatcgttac ggtgcttaga gcctttaaat tcagctgcgg gcaaaatcaa
    21781 actcataaaa taatctgaac aaaagaattg taattatttt gttcaaacac tgattttgct
    21841 aatccttcgc tcggattaaa aggctctaca gcacctacac ttatgccctt taccaatact
    21901 ttcttcagat aaattaaatg caccttctat taatccttca aaacatatgc tattattaac
    21961 atatgatgct ttatatatta ataatatagt tttccttcat taagaaaatt aaggatgcat
    22021 tgaaaggggt tttcactatg agagaatttc aggttttatt gtatactgta aatggagata
    22081 tattcggcac ttatgcagat gaagtagttg aaattataga tagtaaaggt ttagctgacg
    22141 gcttcattac tgtaagagat gtaagagtcc cggttatcaa acttggcaca tttttaggta
    22201 aaggtgacac aactgtttca actgattcga aagtaattct tgtaaaccag aatggccatt
    22261 atgtaggatt tcttgttgac agtgtaacag aacttataag acttcaggaa aactccgtac
    22321 acaatgcacc agaaatacta aaaactcctg aaaacaggtt tatagtaaaa tttggtattg
    22381 ctggggaaca gccgtttcca ataatcaagc tttcatatat tgatgttgag cgcgtttatt
    22441 gaataaaaag aaatatatat caggggctgt cgttatttcc ttagatttat tagggacata
    22501 cctcaacgta aaacggatat tacctgctag agagaggtac gtcccctaac cttactcaaa
    22561 ttttccgcta atgaagtaat ccattaattt gcagccagtt cctacgaata gtccgctttt
    22621 tttagcttca aattcgtcat agcaggagac agcagaatat gaaggttcac atgattcatt
    22681 attaatgcct tcaccataca ttacaaatgg tacaggatct gatgcgtgag ttcttatagc
    22741 cagtggagtg gggtgatccg ggagcaaaag cattctgtaa tctccaatat ttttgaggcc
    22801 tgcaagaagc cgtccaatta cttgctcatc aattaattcg atagatttta ccttgttgtc
    22861 aatttcatat ctatgtccac attcatcagg agcttcaatg tgaatatata caaaatcttg
    22921 accggcttca agttcattta atgcagcatc agctttaccg gcaaaattag tattcaaatt
    22981 acctgttgcc ccttgtacat ttatagaact aagccccatg catataccaa gtccttttac
    23041 taaatcaaca gcagatataa ctgaaccctt tataccatac ttttcgtaaa atcccggtac
    23101 cgaaggtttt ttgccttctc cccataacca tatggaatta gctgggttta aaccgcgttt
    23161 taatctttct atattgattt tatgtccctt caatatatta tagctgtcct ccatcatttt
    23221 gaacaaaata gaggaatttt taccttcagg aatgaatgaa gcaatatttt tacccgaaat
    23281 atcatgcgga ggagtaagct tgaagccatc tggtgcattt tcccaaacca tacaatgcct
    23341 gtaacttact ccgggataga atgaaatttc agatgattgg aaataattgt tgatttcctt
    23401 tatcaattct gctccttctt tagaagatat ttcgtctgaa ctgtagtcca ccatggtttt
    23461 atccatataa ttatctccat cagaaagtgt taccagatta catctgaaag ccttatcgtt
    23521 atcaccaagt tttatgccca tacttactgc ctcaagggga gatcttcctg tgtaatactt
    23581 ttcagggtca tagcccatta cagaaaggtt ggctacatca ctgccgggag ccatgccatc
    23641 aggaattgtt tttaccatac ctgagatgcc ctttgatgca atataatcca tattgggctt
    23701 aactgcacat tgtaaagggg ttttaccacc aagttgcggt acgggataat cagccatacc
    23761 atcaccaagt ataactatat atttcatatt tttattttcc ttttcaaaat attttatatt
    23821 aatatattac cattttgatt aacaagctta taacttatat attatagcag aaacttgcat
    23881 ctgaggcaaa gaaaaattgc aaaataaaaa caaattacgt ataatatata aaagaatatt
    23941 ttgcaagctt gggagtatgt tgttatgaag aaaattactg gtttatttga acaaaaatta
    24001 agattaaagg gatatagggt tacaggtcaa cggcaagctg tgcttgatgt tattgtaaat
    24061 aatgagggta gacatctatg cagcgaggaa atttacgaac ttgtaaaagc ggattttcct
    24121 gaaattggtt tagcaacggt ttatagaaca ctttttctct tagaaaacat ggggattatt
    24181 tacaaaatag acctggatga cggtaaagga agatatgagc tgaataaagg cggggaggat
    24241 catcttcatc atcacttaat atgtactgaa tgcggctcca tttctgaagt taattatgat
    24301 ctcctggaat ttttggagga gcagatttat aataaaaacg attttttagt gaaaaaccat
    24361 agtgttaaat tttatggtat ttgtggaaag tgtttaaaaa ctttgccgta gaaaaaagac
    24421 agccatactg gatataaaga tagtaacaaa ggggttcatg g
//
LOCUS       DAOSDD010000034        24196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_933_length_24196_cov_5.088977, whole genome shotgun sequence.
ACCESSION   DAOSDD010000034 DAOSDD010000000
VERSION     DAOSDD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24196
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_933_length_24196_cov_5.088977"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..658
                     /locus_tag="PK733_04940"
     CDS             26..658
                     /locus_tag="PK733_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOJ09923.1"
                     /translation="MSDIKLNISIKEENNILFKRCIRKLLESTFIVGDKDEKLYAFIS
                     RESNRQDISDYLRMIGFDVLVDTNVKIAMLKTHEADEDVVGLKRANILTFTTEQYHLL
                     LVLWEVYLENLGYNEENLVMRGDLIDKIKAYEVDIDSRRLSAAMDLFKRYNLIDFDTK
                     DKTEDAIITLYPSLQFGWDMAQFQTVSAEYMKDGGLQEEQEDLEEEEDEE"
     gene            655..3957
                     /locus_tag="PK733_04945"
     CDS             655..3957
                     /locus_tag="PK733_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOJ09924.1"
                     /translation="MILLKSVRLVNWYGFNYITVPIGYFSLIAGKNGNGKSVLLDAIK
                     YALYGDTVFNKSTENKGSRTLSSYTRGLLDATAGTYMRPADQFPNVYTHIVLEMFEEE
                     MKRNFVLGTVIETNSANGLIANRYIIENSRLTDVDHVYEENGLTIPYDGIELQKKYGF
                     KMLDAMAGIGKFMQRTGLRLNTQQIVPFRRKLRSIMSYDPDAKIDQFIRESVLEEKKV
                     DFSKLVEAKNNIETLNTTFEKIDTEIKELEEILALFDGLQNARNTIITDDVKIAYREL
                     LVIRRDIDEAIKSIDLAKRQLEEDGKKLEVIQKQEKDIQGRLKKVEDSLDAMDCIKAI
                     RAAEERVEKLTSQKEELNLERKNLEVFQTKISELINWFDEKNISVNNRLILSSLTATE
                     YTAAQKANGIKAFSVQIQNVRDDLLAAITRLRDETNKNRSMQIQQQRLIDDCNDKKTT
                     FSEIPDYVALKDEINKEFAKRHIRSEAKFACEYVIALKDERWRDAIESFLGKRRYTIL
                     VEPEYYDIADDVLNESPHRYAHLFNTKLLMKKKLSPEEDSVVRYIEVKNSVAKQYFEY
                     QLGRMHAVTKEQVRNFENAISKEGRVSVAMDSYFLQFDRIRFYCLGQETIELNRIRAE
                     KRLQTLVKEGETLRERTHEQEQKKGYLETAQRYFGNYNYDAYQMCEDILRKCEVAAKE
                     LEDLKQAQQNNMEYLEMNQLYTKYKGELEANGKERGKVQESQVKMNTQLEVNQEKEIE
                     KTKELEKYNGIMNEYQRQNYDVYKKAIEDYDKYISNDETGTGGTLKDRERAERNLKDA
                     GKKLIGAQSIYNAHRNVESRLLTGEDQRFSYEARKTQIWMDDLQEIKEKLKEQTRRYE
                     EIFKNEFVLTVMKSCESAIDDLKLINAELSRLKFKSKYEFEVKYVKDGSDYEKILEYA
                     KYLKERQELGITSTQMTLSTLTTFTDDEGIELEKEIRKIINRIIDSNDKEQIEKYADY
                     RNYMVYEILLTNDVLNKAKLSKQSGFNSGAEVQIPYMLILLSALLMIYNDKLNSTRLV
                     FIDEPFAKMDPINVKIMLDFMKKQKLQMIFCSPDKTELIGNECNVILPVLRTRSNLME
                     IGIVEIHEECSL"
     gene            3954..5165
                     /locus_tag="PK733_04950"
     CDS             3954..5165
                     /locus_tag="PK733_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOJ09925.1"
                     /translation="MNWEEKILVSLVEKYRGSKKDTGDNKTNRRTQVKPDRLYKKYKA
                     NDGDFDVITTINHTVEKLCRIGFLTRDQEKFGTLLQCIYLVDEQIEQVEDYLHKKYGF
                     IPKGMKKNNVQDIISKYHDLSEICSMECERLRRELDRNKIPNDYETLPKILDAVAFIE
                     NNRTELFVREASMKVYGDSKYFEENTLVPVCQMLRKYKNKPCNTDELMDEILSDYMIK
                     KEPQRFSIKGDFILSIYGKELDFSVLPDGIELDSNSLKKIDCIKIRTQKFMTIENRTS
                     YLRYSAPDTVTFYLGGYANRFQRDFIKKVYDDNPDIKFLHFGDIDAGGFWIHHNLCEI
                     TGVNFKTFCMSKDELSQKQYERCLHRLTGNDTVRLQELKEMRAYADTIQYMLQHNVKM
                     EQEIISLELMR"
     gene            5614..6201
                     /locus_tag="PK733_04955"
     CDS             5614..6201
                     /locus_tag="PK733_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ09926.1"
                     /translation="MIIWLNGAFGSGKTTCAFELNRRLPNSFVYDPENIGYFIRNNTP
                     KEIHKSDFQDHGQWRLFNYEMLKYIATEYLGIIIVPMTINNHQYYEEIIQRLIDDGII
                     LKHYILYANKETILKRLNKRLERGETWAKSQIDRCIEVFDTEITEEKIITDNRNIDYV
                     VEEIAKISGVTLLPDKRTFLKKGVDRAITLIKHIR"
     gene            6500..6667
                     /locus_tag="PK733_04960"
     CDS             6500..6667
                     /locus_tag="PK733_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09927.1"
                     /translation="MPKDNRNKRMYTEEQREAHAERWTRETRNWKLPDIVALNPTEEI
                     KEIAKKQDSLA"
     gene            complement(6799..7857)
                     /locus_tag="PK733_04965"
     CDS             complement(6799..7857)
                     /locus_tag="PK733_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ09928.1"
                     /translation="MMKRSAKVDGIRISRYVSRFLNDYAPMNLTSSEATMNGYRIALG
                     SYIGWLEDEKKVTPSTLCAACFEAKLIEEWMGWLKTTKANSNDTCNVRLGALRSFISF
                     LGKQEPEYRYLPAEATCIRRMKKKGRNVSGLTREAVKALLEAPDTSTQSSRRYLAMMI
                     LLYSLAARLDEVLSLRINDIHLDISKPCITLTGKGRKVRTLGLLAKSVGHLEKYILET
                     HGAFPEKDAYLFYTKHKGKYEKISQTAVRKALRKYAEIANRQCREVPLTLHAHQFRHA
                     KAEHMLEDGINIVQISEYLGHADLDTTKVYLEVTDRMKAEAFATMAGENDKEVSRKWK
                     NKDGSLRDFIGITRKGFV"
     gene            complement(7832..8752)
                     /locus_tag="PK733_04970"
     CDS             complement(7832..8752)
                     /locus_tag="PK733_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ09929.1"
                     /translation="MGTWSDTIEPLLHAFDTHCDRLDDGNGILTQEMVNSWCAKKDTE
                     TNNSCRARVNPAMGFLGYLINRGKADISIPALPKWGKCTYIPTVLTHTELERSFYECD
                     HISSMRNHSISGRNRKMVIPVIFRFLFSTGMRPFEVRWLRVKDVDLSTGIVSIERTKR
                     GIQHRIVLHDSMLELAKEFDSHIRSIYPDREYFFPGFSGGCHTAQWLRETFRTLVLKS
                     GGPKRAVPYSFRHNYATENLNRWDGTDSDFFYRFVMLPKSMGHTGLKSTQYYYRKVPK
                     FYELIEEKTGGGMECIIPDLEVDDETFGKG"
     gene            complement(8802..10031)
                     /locus_tag="PK733_04975"
     CDS             complement(8802..10031)
                     /locus_tag="PK733_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ09930.1"
                     /translation="MDITHLKEAYPKLIRYMTNLGYSSSYIGLVKSAVGFITEHPNNT
                     WNSYDDVCTDYIGERTGWEAIKRKSRINLVANFDLDNRFPERRVHIKVQTKANKSLCP
                     EFQCLLDCYEQFCDKKGVGESTKASRLYAGRGFFSRLQKAGCSNLLTVTENDVLEGLK
                     NKNGVLFSHRYCLYIKDILECGVNVDEASCIRIQGYIPRIRSGRKNIQYMTEAEAGSV
                     SEVLTSGASGLSFREKAVGSLLLYTGLRRSDIAGLDFLSIDWGSEMINVTQQKTGIPV
                     RLPLLPAVGNALYDYLTCERPAADIPYIFVTLRSPFRAIGARGIERISNRIFDTAGIR
                     TQKGDRRGTHIFRHRLVAKMLENDVPQPIITEILGHSSPRALDDYLNTDFRHLKECAL
                     SIEDFPVSREVFEDEEL"
     gene            10361..10507
                     /locus_tag="PK733_04980"
     CDS             10361..10507
                     /locus_tag="PK733_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09931.1"
                     /translation="MPVDMPVEARCQEDRGINAERMYDYRIQIRIFRNGKKRIEYKTG
                     QLPF"
     gene            complement(10836..12299)
                     /locus_tag="PK733_04985"
     CDS             complement(10836..12299)
                     /locus_tag="PK733_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ09932.1"
                     /translation="MSDNRIGKDAIKLTVSKVMTMIISMLTAILLSRFRTLEEYGTYS
                     QILLVVNLMTSLIMLGLPNSINYFLARAENDDERNKFLSVYYTISTILSIMVDLILVC
                     SISYIVKYFNNELLKNFVFVFAIFPWTKIIMESVDNVLVIYKKTSMIMFYRISNSIAL
                     LILIFIAQLYNWDFHVYMILFVFVEAIFAISVYVIVKNIASKLTIKFDKWIIINVLSF
                     SIPLGLASVAGTLNIELDKLMIGKFFNTEQLAIFANASKEMPITIVATSLTAVLLPQL
                     ARLLKNNQHQEAVNLWGSATTLSYLIICFLATGFFTYAPDLITLLYSEKYLPGVSVFR
                     IYTIVLLLRCTYFGMILNAKGKTKFIFYSSIISLVLNIVFNYILFKAIGFIGPAIATF
                     LSQFIINLVQLIFTAKIVQVRFIKILPWKNIGFITIVNVLLGLLFYHIKYFIRLDIII
                     GSLFETILLGVLWGVIYLIVFMKEIKKQWLSLNEEGK"
     gene            12631..13767
                     /locus_tag="PK733_04990"
     CDS             12631..13767
                     /locus_tag="PK733_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09933.1"
                     /translation="MVEIIKSISKLYLLNLDNPLPVIKPLYPDFGRSAKNQQSIIRSL
                     ILMLDQQVYSITKWSKRVASDTLIFYICGFEDRAPIVASYYDLLVRLWMKERKFHLSR
                     KLKVKHFVRKPSKKLKAGQKLPPKHSGSVKKLVKKAVKGKLKNFCPERILQKLLARCV
                     VDTSAQMGILGNNSNLSVAFDSSPFYSGASDYGVKVCDCKSKGIYNCECPRRFSDPDA
                     KWGWDSYREQWFFGDTLFNVTASDSPYDLPISIKIVQASRHDSILTVFALDDIHNLYP
                     NIRFRYFIADGAMDNYPTYDLLKYYDMLPFILMDSKTKAKLNYSHPDILCFDDKGHRI
                     CNGGIPLVNWGYSNPKGIKYRCWFAVKGLSHLQIVNAPIVVMAM"
     gene            13767..14036
                     /locus_tag="PK733_04995"
     CDS             13767..14036
                     /locus_tag="PK733_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09934.1"
                     /translation="MYIKPDFDPRMFPPVPCYSEAYKNKFKTRTSVERFNKRMFEDYA
                     LKYYGSRSSMMRASLATFTVVNIHLDAWIKHICFNFINIIEKQAA"
     gene            complement(14434..16224)
                     /locus_tag="PK733_05000"
     CDS             complement(14434..16224)
                     /locus_tag="PK733_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ09935.1"
                     /translation="MKKVIQNNMLMLKYVARFCPSHIFITILNAILASIISVFNILFQ
                     RHVVNTITKSTNFKVVSLSVLGITIVNITYSFINIWLQQRIIPRNTQILRQKMQMEIF
                     RKAMEVDYECYEDSEYFNKFTLATQQAGSRAVAVLSTFSSLIGSLSGIGALVTLISCL
                     DPIILMFVITNVAIYFYCSTKTIKIQHKYYEEKIPLEREIGYAQRVFYQREYAKDIRL
                     FDKLSSLIKSIFNTSSNNLISLIGTYGKKLSKYLGSHSILGSGVNSAIMLYLSYRVLD
                     KGLSIADFIALWSSSQQLSHQISQLLDIFPKLYENSIYIDNFIGLINYTSQISENHNS
                     IDATNVQSIEFKDVTFIYPNTDKIVLKNINIKISSGEKVAFVGRNGAGKSSLVKLITR
                     LYDPTEGRILLNDTLYQNYTVSSLRNNIGIVFQDYQTYAMTIAENVLMRPMINKAEDE
                     KLVNNALKFVGLYDKVNSISMGMYTVLTREFKNTGAIFSGGELQKLAIARIYVKNCSI
                     IILDEPSSALDPIAENEILNSVLDLASNKTVILISHRLANVKNVDRIFFIEDGLLVES
                     GSHDELMNMNGKYAEMYKIQVDQYDILMNK"
     gene            complement(16665..16920)
                     /locus_tag="PK733_05005"
                     /pseudo
     CDS             complement(16665..16920)
                     /locus_tag="PK733_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611566.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(17591..17848)
                     /locus_tag="PK733_05010"
     CDS             complement(17591..17848)
                     /locus_tag="PK733_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXXX repeat peptide modification system protein"
                     /protein_id="HOJ09936.1"
                     /translation="MNKVVWKLSDDEKKEIQNLFEKKIALENLVKIIDPANEKLYNKL
                     ISDYGTTVRLFEQWWTVTSQKNKWEGKNWRINFETNEVLAQ"
     gene            18164..19471
                     /locus_tag="PK733_05015"
                     /pseudo
     CDS             18164..19471
                     /locus_tag="PK733_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603477.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20346..22520)
                     /locus_tag="PK733_05020"
     CDS             complement(20346..22520)
                     /locus_tag="PK733_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM peptide maturase, CXXX-repeat target
                     family"
                     /protein_id="HOJ09937.1"
                     /translation="MNYSILFKDTKRKEYKVGKFIPAWKDSNAQSITFIITEDCNLRC
                     KYCYITHKSSNKKMSLETAKKFINYIFAGNISTNEAVIIEFIGGEPFIEIELIDQISD
                     YFKIVAFEKDHDWYWNYRINICTNGVNYSDEHIQNFINKNMGKLSVSITLDGTKEKHD
                     MHRVFPNGSGSYELIKKNIPLWISQFGGSTKVTFASSDLKFIKESIISLWNNGITNIA
                     SNVVFENVWQEGDDLLFEKQLVELADYVLHNKLYDKYYCTFFDENIGGYYNSDDLNKT
                     YCGAGKMIALGSNGNIYPCLRYKDYSLNNKKEWVIGTVDDGIDMEKVRPFITAMIKLQ
                     SDEECINCEVASGCGFCQGFNYDEANTPTNFSRAKYICKMHKARVRANDYYFSKLFNL
                     YGIERDALKRDHKSLYFLLSDDYITYCQHKNTSVTANTMNYHDIIIGLKYARSNFFSP
                     IFVHSSNEFNFANIKEYEQYRILHIVPAKFHKEASQLRDCLYVFEEDDLNLSIRGISK
                     CMLNIDSKEIENLSYYVLKLLNIVKRINVNVLNLDENFNEAEYRKQLITIKNRLVEFF
                     MTTGLKQEINLITDLCYINERNNCKAGERSFSYAPDGRLYVCSAYYSSREDSHIANIG
                     EEINRFKNEHLYKTSYNALCSLCDAYQCINCIYLNESATNEVNVSPSYQCRKSYIERE
                     ISRQYQVETNWNIEDIISSIKYKDPMSIYIDNVGALTGYYKY"
BASE COUNT         8005 a         4220 c         4102 g         7869 t
ORIGIN      
        1 atattagaat aaaaagggtg agataatgag cgacattaaa cttaatatat caattaaaga
       61 agaaaacaat attttgttta agcgatgtat acgtaaactt ttagagtcca catttattgt
      121 gggcgataaa gatgaaaaac tatatgcatt tatctcaaga gaatctaata gacaagatat
      181 ttcggactat ctgcgaatga ttggttttga tgtattagta gatactaatg tgaagattgc
      241 tatgctaaag acacatgaag cagatgaaga tgttgtaggc cttaagagag caaatatttt
      301 aacctttaca acagagcaat atcatttgct tttggttctt tgggaggttt atctggagaa
      361 cctcggatat aatgaagaaa atttagttat gcgtggtgat ctgattgata aaattaaagc
      421 ttatgaggtt gatatagaca gtagaagatt gtctgcagca atggatttat ttaaacgata
      481 taacttaatc gattttgata caaaggataa aacagaagat gcaatcatca ccctgtatcc
      541 ttcgttacaa tttggctggg atatggcaca gtttcagacc gtatcggctg aatatatgaa
      601 agatggagga ttacaagagg agcaggaaga cttggaagaa gaggaggatg aggaatgata
      661 ttattaaaat ccgttcgtct ggtgaactgg tatggcttta attatataac agttcccatt
      721 ggatactttt ctttaattgc cgggaaaaat ggtaatggta agtcggttct gttagatgct
      781 attaagtatg ctctatatgg agatacagta tttaataaat ccacagagaa caaaggaagc
      841 agaactttgt cttcttacac cagaggatta ttggatgcaa cggcgggaac atatatgagg
      901 cctgcagacc agtttccgaa tgtatatact cacatcgtat tagagatgtt tgaggaagaa
      961 atgaaacgga attttgtgct tggtactgta atagagacaa attctgcaaa tggtcttatt
     1021 gcaaatcgct atattattga aaattcaagg ttaactgatg tagatcatgt atatgaagaa
     1081 aatggcttga ccattccata tgatggtatt gaattacaga aaaaatacgg ctttaagatg
     1141 ctggacgcaa tggctggtat tggaaagttt atgcaaagga cggggttgag attaaacact
     1201 caacagatag taccgtttcg aaggaaacta cgcagtatta tgtcttatga cccagatgcg
     1261 aagatagacc agtttattag agaaagtgtc cttgaagaga aaaaggtaga cttttcaaaa
     1321 ctggtagaag ccaagaataa tattgaaaca ttgaatacca catttgagaa aatagataca
     1381 gagatcaaag aacttgagga aatacttgct ttgtttgatg gattgcagaa tgccagaaat
     1441 actattatta ctgatgatgt gaaaattgct tatagagagc tgcttgtaat cagacgagat
     1501 attgatgaag cgataaaatc tattgatctg gcaaagagac aattggaaga ggatggtaaa
     1561 aagctggaag tgattcaaaa acaagaaaaa gatattcaag gcaggcttaa aaaagtggaa
     1621 gacagcttag atgctatgga ttgtataaag gcaattcgtg ctgctgagga acgagtggaa
     1681 aaacttactt cgcagaaaga ggaattaaat ctagagagaa aaaacttaga ggtattccaa
     1741 acaaagataa gtgaattaat taattggttt gatgaaaaaa atatatctgt taataaccgg
     1801 ttaattttat catcgcttac agcaaccgaa tacacggcag ctcaaaaagc aaatggtatt
     1861 aaggcattct cagttcaaat tcaaaatgtg agagatgatc tcttggcagc aatcacaaga
     1921 cttcgggatg agacaaataa gaatcgatct atgcagatac agcagcagag attaattgac
     1981 gattgcaatg acaaaaaaac taccttctcc gaaatcccgg attatgttgc attaaaggat
     2041 gaaataaata aggaatttgc taaaagacat atccgttcag aagcaaagtt tgcctgcgaa
     2101 tatgtaattg cacttaagga tgaaagatgg agagatgcga ttgaatcttt tcttggcaag
     2161 agaaggtaca cgattcttgt ggaaccggaa tattacgata ttgcagatga tgtgttaaat
     2221 gaatctccac acagatatgc acatttattt aatacaaaat tattaatgaa aaagaaactg
     2281 tctcctgaag aagattcggt tgtgcgctat attgaagtaa aaaattctgt ggcaaaacag
     2341 tattttgaat accaattagg tcgcatgcat gcagtgacaa aggaacaagt tcgtaatttt
     2401 gagaatgcta tttctaaaga gggaagggta tccgttgcta tggatagtta cttcctgcaa
     2461 tttgatagaa tacgttttta ttgcttggga caggagacaa tagagcttaa tcgaattcgt
     2521 gcagaaaaaa gattacagac tctagttaag gaaggggaaa ccttacggga acggacacat
     2581 gaacaggaac agaagaaagg gtatttagag acagcgcaaa gatattttgg caactataat
     2641 tatgatgcat atcagatgtg tgaggatatt ttgcgtaaat gtgaggtagc agccaaggag
     2701 cttgaagact tgaaacaagc gcagcaaaat aatatggaat atctggaaat gaatcagctt
     2761 tacacaaagt ataaaggaga gcttgaagca aatggcaaag agcgtggtaa agttcaagaa
     2821 tctcaggtaa agatgaatac acaactggaa gtaaatcaag aaaaggagat agaaaagact
     2881 aaagagttag aaaaatataa tggtattatg aatgaatacc aaagacagaa ttatgatgta
     2941 tataaaaagg ccattgaaga ttatgacaaa tatatatcaa atgacgaaac tggtaccggt
     3001 ggtactttaa aagatcgtga acgagccgag cgtaatctta aggacgctgg aaaaaaactg
     3061 ataggtgcgc agtctattta taacgctcat agaaacgtag aaagtagact tttaaccgga
     3121 gaggatcaac gtttttcata tgaggcaaga aaaactcaaa tttggatgga tgatctccaa
     3181 gaaatcaaag aaaaactcaa agagcagact agacgatacg aggaaatatt caagaacgaa
     3241 tttgtactga cggtaatgaa atcatgtgaa agcgcaatag atgacttgaa attaatcaat
     3301 gcagaacttt ccagattaaa attcaaatca aaatatgagt ttgaagtgaa atatgtaaaa
     3361 gatggttctg attatgagaa gatcttggag tatgcaaagt atttaaagga gcgtcaggag
     3421 ttaggaataa cttcaactca aatgacatta tcgacactta ctacattcac tgatgatgaa
     3481 ggaattgaat tagaaaagga aattcgtaaa attattaatc gcattattga cagtaatgat
     3541 aaggagcaaa ttgaaaaata tgctgattac agaaactata tggtatacga aattttactt
     3601 acaaatgatg tgttaaataa ggctaaactt tccaaacaat ctggttttaa ttcgggggct
     3661 gaagtacaga ttccatatat gttgatttta ttatctgcgt tattgatgat atataatgac
     3721 aaattaaatt ctacaagact tgtgtttata gatgagccat ttgctaagat ggatcctatt
     3781 aatgttaaaa ttatgcttga ttttatgaag aaacagaagc tgcagatgat tttctgttca
     3841 ccggataaga cagagttaat tggtaatgaa tgtaatgtaa ttcttccggt gcttagaaca
     3901 agatcaaatt taatggaaat aggaattgtt gaaatccatg aggagtgtag cctatgaatt
     3961 gggaagagaa gatacttgtt tctctggtag aaaagtatcg gggaagcaag aaagataccg
     4021 gggataataa aaccaaccgc agaactcagg taaagccaga tagactttat aagaaatata
     4081 aggcaaatga tggtgatttc gatgtaatca ctacaataaa ccatacagtg gagaagctct
     4141 gcaggatagg ttttctaacc cgtgatcaag aaaaatttgg tacactgctt caatgtattt
     4201 atcttgtgga tgagcagatt gaacaggttg aggattatct acataaaaag tatggtttta
     4261 ttccgaaagg aatgaagaaa aataatgtcc aggatatcat ttccaaatat catgacttat
     4321 cagaaatctg tagtatggaa tgcgaacgct tgcgaaggga gcttgataga aataaaatcc
     4381 caaatgatta tgaaacactt cctaaaatat tagatgcggt tgcttttata gagaataatc
     4441 gaacagaatt gtttgtaaga gaagcttcta tgaaagttta tggggattcg aaatattttg
     4501 aagagaatac attggtaccg gtttgccaga tgcttcgcaa atataaaaat aaaccatgta
     4561 acacagatga actgatggac gaaattttat cagactatat gataaaaaaa gaacctcaaa
     4621 gatttagcat taaaggtgat tttatattat ctatttacgg aaaggaactg gattttagcg
     4681 tacttccgga tggcatcgag ctggatagta attctcttaa aaaaattgat tgtatcaaga
     4741 taagaacgca gaaatttatg acgattgaaa atagaacttc ttatctgaga tattctgctc
     4801 ctgatacagt cacattttac ctgggaggat atgcaaatcg ttttcaacgt gattttataa
     4861 aaaaagtata tgatgataat cctgatataa aattcttgca ctttggtgat attgatgccg
     4921 ggggattttg gattcatcat aatctttgcg aaataacggg tgttaatttt aagacatttt
     4981 gtatgtcgaa ggacgaactt tcacagaaac aatatgaaag atgtctccat cgattaacag
     5041 gcaatgatac tgttagacta caagaattga aggaaatgag agcgtatgca gatactattc
     5101 agtatatgct tcagcacaat gtaaaaatgg aacaagagat tattagttta gagctgatga
     5161 ggtaaagaat attatagcgt ttttaagtat ttataagtat atttttcatg ctataatcag
     5221 cggtgaattt attaaaaata agtgttgagc gcagattaac tgaaaatata cccttacacc
     5281 aagggggtgc caggcacgat ggtcatcatt attgcacact cagggcacgt tgaagcagca
     5341 attctgatga cgtattgtgg ttctgataag gaataatggg ctttgaccac aacatatagt
     5401 ggtttgagag tgaaaatagg gtcaaaaatc agtgaaaaaa caccgttttt tgacccttaa
     5461 aaacaaggcg tttgacagat gacattggat gtttggagat atttgaagtg aagatgggta
     5521 aatttggttg atattatagt aggcgaatta tatactatag atgaacattt gtatatttat
     5581 gtgaagtaaa attatgctaa aggaggatat ataatgatta tttggttaaa tggtgcattt
     5641 ggttctggaa aaacgacatg tgcctttgaa ttaaatagaa gacttcctaa ttcgtttgtt
     5701 tatgaccctg aaaatatcgg atattttatc cgtaataata caccaaaaga gattcacaag
     5761 tctgattttc aagaccatgg acaatggcga ttatttaatt atgaaatgtt aaaatatatt
     5821 gccacggaat atttgggtat aattattgta cccatgacca tcaataatca tcaatattat
     5881 gaggagataa tccaaagact catagatgac ggtataattc taaagcatta tattttgtat
     5941 gctaataaag aaaccatatt aaaacgattg aataaacgac ttgaacgagg tgaaacttgg
     6001 gcaaaatctc aaattgatcg ttgtattgaa gtttttgata ctgaaattac tgaagaaaaa
     6061 atcataacag ataatagaaa tattgactat gtcgttgaag aaattgcaaa aataagtgga
     6121 gtaactcttt tgccagacaa aagaacattt ttgaaaaaag gggttgatag agcaataacc
     6181 ttaataaaac atatacgtta ataaatggat tttatcaatt acgacgcata tatcaagtat
     6241 tatgcgcaac taataagata taaagaaaat gcaaactata gatgtattag agtataaaga
     6301 attctaatgc atctattttt atataaagaa aattatatac agaatataaa gctggtgaag
     6361 ttttaggtca agaagacctt agcgaacttc gccatttttg gtactataaa aagtgtaaat
     6421 gccccctgtg ttaggatagt tgtcgtaaga taaattttat agtattctcg taataagaga
     6481 gaattaacag gagacgacta tgccaaaaga taacagaaat aagagaatgt acaccgaaga
     6541 acagagagaa gcccatgcag agcgttggac aagagaaaca cgtaattgga agttaccaga
     6601 tattgtagca ttaaacccta cagaggaaat aaaagagata gccaaaaagc aggatagtct
     6661 ggcttgaata aaaaaagtag cttttatgcc gcttatcaga acttttaaga aatcagaact
     6721 tttttcaact ggtccctgaa taaaaagttt ccagtcatct tcaatgatag aaaaagttct
     6781 gattttcctt ccatacttct atacaaatcc cttccgggta attccaatga aatcccttag
     6841 tgatccatct ttatttttcc attttctaga gacctcttta tcattttcgc ctgccattgt
     6901 tgcaaatgcc tctgctttca tacggtctgt aacctctagg tacacttttg tcgtatctaa
     6961 atcggcatga cccaaatatt cggatatctg gacgatatta attccatcct caagcatatg
     7021 ttcggctttc gcatgacgaa attgatgggc atgaagtgtc aatggcactt ccctgcactg
     7081 tctgtttgcg atctccgcat attttcgaag tgcttttctg actgcagtct ggctgatttt
     7141 ctcatatttc cctttatgtt tggtatagaa caggtacgca tccttttccg gaaaggcacc
     7201 gtgagtctca aggatatatt tctcaagatg tccaaccgat tttgcaagca gcccaagtgt
     7261 ccttaccttc ctacccttcc cggtaagtgt gatgcacggt ttggaaatat ccaggtggat
     7321 atcatttatg cgtagggaca ggacttcatc cagcctagca gccagactat acagaagaat
     7381 catcatagca agatatctac ggctggactg cgttgatgta tcaggagctt ccagaagagc
     7441 ttttactgct tcacgtgtga gcccactgac atttcttcct tttttcttca ttcgccgtat
     7501 acatgttgcc tccgcaggta aatatctgta ttcgggttcc tgcttcccaa gaaaggatat
     7561 aaatgaacgc agtgccccaa ggcggacgtt acaggtatca ttgctgtttg cctttgttgt
     7621 cttcagccag cccatccact cctcaataag ttttgcttca aaacaagctg cacaaagagt
     7681 ggacggagtg acttttttct catcctcaag ccatcctata tatgaaccca gagcaattct
     7741 atacccattc atggttgctt cagaagatgt caggttcata ggggcataat catttaaaaa
     7801 ccgtgagacg tatcgggaga tccggatccc atcaaccttt gccgaacgtt tcatcatcca
     7861 cctccaaatc cgggatgatg cactccatgc ctccccctgt tttctcttct atcagctcgt
     7921 agaactttgg aactttccgg tagtaatact gtgtgctttt cagaccagtg tgtcccatgc
     7981 ttttgggcag catcacaaac ctgtaaaaaa aatcagaatc tgttccatcc catctgttca
     8041 ggttctcagt tgcataatta tggcggaaag aatacggaac agccctttta gggcctccgg
     8101 atttcaacac caatgtcctg aaggtttccc gaagccattg cgcagtatgg catccgcctg
     8161 aaaaccccgg gaaaaagtat tccctgtccg ggtaaatgct gcggatatgg ctgtcaaatt
     8221 cttttgccag ttccagcatg ctgtcatgaa gaacaattct atgttggatg cctcttttcg
     8281 ttctctcaat actgacgatt cctgtactga gatcaacgtc tttcacccgg agccaccgaa
     8341 cctcaaaagg ccgcattccc gtactgaaaa ggaaacggaa gattaccggg attaccattt
     8401 ttctattcct tccggatata gaatgattcc gcatggaaga aatatgatca cattcataaa
     8461 aggagcgctc aagttctgta tgagttaaaa ccgtggggat gtacgtgcat tttccccatt
     8521 tgggaagggc tggaatggag atgtcagcct ttcctctgtt tattagatag cccaggaatc
     8581 ccatggccgg attaacccgt gccctgcagc tgttgttggt ctcagtgtct ttcttggcac
     8641 accagctgtt gaccatttcc tgggtcagta tgccgtttcc gtcatctaat ctgtcgcaat
     8701 gggtatcaaa tgcatgaagc aacggttcaa tggtgtctga ccatgtaccc aagtccatgc
     8761 gccatttttt atattctatg aataagggcg aaagaaaaga cttataattc ctcatcctcg
     8821 aacacctccc tgctgaccgg aaaatcctct atagaaagcg cacattcctt caaatggcga
     8881 aaatccgtgt tcagatagtc atcgagtgcc ctcggacttg aatgtccaag gatttctgtg
     8941 atgattggct gcggcacatc attttcaagc atctttgcca caaggcggtg ccggaaaata
     9001 tgcgttcccc ggcggtctcc cttttgcgtg cgtattcctg cggtatcaaa aatacggttc
     9061 gaaattctct cgatacctct agctccaatt gcccggaaag gagatcttaa cgttacaaaa
     9121 atatacggta tgtcagccgc agggcgttca caggttaaat aatcgtaaag cgcatttcct
     9181 acagcaggga ggaggggaag cctgacggga atcccggtct tttgctgagt gacatttatc
     9241 atttctgaac cccagtctat ggataaaaag tccaaacctg ctatatcgct tctcctaagg
     9301 cccgtataca gcaacagaga gccgacagcc ttttcacgga aagacaaccc tgaggcccct
     9361 gaagtcaaca cttccgaaac agacccggct tctgcctctg tcatgtactg gatgtttttt
     9421 cttccactgc ggatccgggg gatgtatccc tgtatcctga tgcaggatgc ttcatctacg
     9481 ttgacgccgc attcaaggat atccttgatg tacaggcaat atctatgcga aaacaataca
     9541 ccgtttttat tctttaatcc ttccagcaca tcattttctg taacagttaa aaggttgctg
     9601 catcctgcct tctgcaaacg cgaaaaaaac cccctgcctg catacagcct tgatgctttt
     9661 gtagactcac caactccttt cttgtcacaa aactgttcat aacagtccag cagacattga
     9721 aactccgggc acaggctttt attagccttt gtctggactt tgatatgcac tcttctttcc
     9781 gggaatctat tgtccagatc aaagtttgcg actaaattta tccgcgattt tctttttatt
     9841 gcctcccagc ctgtccgttc tccgatatag tcagtgcata cgtcatcata tgaattccat
     9901 gtgttattcg gatgttctgt gatgaaacca acagcgctct ttactaatcc aatgtagctg
     9961 gaagagtatc ccaaattcgt catatatctg atcagcttcg gataggcttc ctttaaatgt
    10021 gtgatatcca tcttctatcc ctccttcatg aaataaggat agcggaaatc ttttgtaaaa
    10081 actagactga taatcaaaat gtttacattg tatgatagaa ggttgactat caccaggctg
    10141 aatgaaatca gaacttatat catagccggc aatgctaaag accacatttt catgccggat
    10201 gttctgattt cttaaaagtt ctgataagcg gtgaaatcag aacattctga ttttgccgcg
    10261 aaagcctctt gacaatgagt tacggcatgt taggctgata tgctaggcat caaccatgct
    10321 ccagagccct tctccttgtt catcatgggg gatgccggtg atgcctgtag acatgccggt
    10381 ggaggctcgt tgtcaagagg atcgtggaat aaatgcggaa agaatgtatg actacagaat
    10441 acagattaga atcttccgga atggcaaaaa gcggattgaa tataaaacag ggcaactgcc
    10501 cttttaaata aaaaactaga ggtaaaaagt gtctgcaagc cactatttca ctgctaaaaa
    10561 aggcgggaat atggaacttg caatactatg gaaaatggag ttgcaatact atggaaaatt
    10621 attttgcaac tttattcaaa aagtacttgc aaaaaacagc ctcatgatta taatataaca
    10681 gatacggaat atttttaata attacgggtt tggaacagta tttcggatgc atccgggttc
    10741 ttcaccaatt ttggttggtt atcttaacct atttgtctgt acaaatttat ggtatcatta
    10801 taccttgtcc cattacattg atgattgaca aaatcctatt taccttcttc atttaaagat
    10861 aaccattgtt tttttatttc tttcataaaa acaatcaaat aaataactcc ccatagtact
    10921 cctagcaata ttgtctcgaa taagctacca attataatat ctaaacgaat aaaatatttt
    10981 atatgataga ataataaacc tagtaagacg tttactattg ttataaaccc tatatttttc
    11041 caaggcaata tttttataaa cctaacttgt acaatcttgg cagtaaaaat cagttgtact
    11101 aaattgataa taaactgact tagaaatgtt gcaattgctg gcccaataaa tcctatagcc
    11161 ttgaataaaa tataattaaa aactatattt aatacgagtg aaataataga actgtagaat
    11221 atgaattttg tcttaccttt tgcattaagt atcattccaa aataagtaca ccttaataag
    11281 agaactatag tataaattct aaaaactgat acacctggta aatatttttc agaatataat
    11341 agtgtaataa gatctggtgc atatgtgaaa aatccggtag cgagaaaaca aattatcaaa
    11401 tatgatagag tcgtagcgct tccccacaaa tttactgctt cttgatgctg attattcttt
    11461 agcagtctag ctaattgagg taataaaact gctgtcagtg atgtagctac aattgtaata
    11521 ggcatttctt tagaggcatt ggcaaaaatc gctagttgct ccgtattaaa aaacttacca
    11581 atcatcaatt tatcaagttc aatatttaaa gtaccagcaa cagaagctag tcctagagga
    11641 atcgaaaacg acaacacatt tataataatc catttatcaa atttaattgt taacttactt
    11701 gctatattct ttacaatgac ataaacacta atagcaaata tagcttcgac aaaaacaaat
    11761 agaatcatat atacatgaaa atcccagttg tataattgag caataaaaat aagaattagt
    11821 aaagctatac tatttgaaat tctataaaac attatcatag aagttttctt gtaaataact
    11881 aaaacattat ctacactttc cataatgatt tttgtccatg gaaatatcgc gaatacaaat
    11941 acgaaatttt taagaagttc attattaaag tactttacta tataagatat agaacaaact
    12001 aaaattagat ctaccatgat actcaaaatt gtactaattg tataatatac agatagaaac
    12061 ttatttcttt catcatcatt ttctgcacgt gccaaaaaat aattaatgct atttggtaat
    12121 cctaacataa ttaatgatgt cattaaattt acaactaata atatttgaga atacgttcca
    12181 tattcttcca atgtccgaaa tcgtgataat aaaatagctg ttaacattga tattatcata
    12241 gtcatgactt ttgaaactgt tagctttatt gcatcttttc caattcgatt gtcactcaag
    12301 aattatatac accccttgta caatatagta tgctctcgaa aagtcggaga gctttaaatt
    12361 tgtatatttc aaggttcagc attttagctt cacccctact ttttagtcaa ttttccttat
    12421 agaatttcca gatatgcgtt accattttat tcttgtatta cattaattta cctcttaaag
    12481 tttattacaa tatccttcaa aacaatatta tgtaaactaa atttattttg gagatatatg
    12541 attatgttac gagtgtggat ccctcatttt gaatatcaga agaaacttta tgttaggatg
    12601 tctattcaat atgaaaaaca aaggagcagg gtggttgaaa taattaagag tatctcgaag
    12661 ctctaccttc tcaacctaga taacccgctc cctgttatca agcctttata ccccgatttt
    12721 ggccgctccg ctaaaaatca gcaaagtata atacgctcac ttatattaat gcttgatcaa
    12781 caagtttatt ctatcacaaa atggtccaaa agggtagcct cagatactct aattttttac
    12841 atttgtggtt ttgaagatag agcacctatt gtagcttctt attatgactt gctagttcgt
    12901 ttatggatga aagaacgtaa atttcactta agcaggaagc ttaaggttaa gcattttgtc
    12961 cgtaaaccaa gtaaaaagct taaagctggt caaaaactac cccctaaaca ttcaggttcg
    13021 gttaaaaaat tagtaaaaaa ggcggtcaaa ggaaaactca aaaatttctg cccagaaaga
    13081 atcctacaga agcttttagc ccgctgtgtc gttgatactt ccgctcaaat gggtatccta
    13141 ggtaataata gcaatttatc tgttgctttt gacagctctc ctttttattc aggtgccagc
    13201 gattatggcg tcaaagtctg tgattgtaaa tctaaaggaa tctataactg tgaatgccca
    13261 agacgctttt ctgaccctga tgcaaaatgg ggttgggata gttatcgtga acaatggttt
    13321 tttggagata cacttttcaa tgttacagct tctgatagtc cttacgatct tcccatctct
    13381 atcaaaatag tacaggccag ccgtcatgat agtattttaa cagttttcgc gcttgatgat
    13441 attcataatt tgtatcccaa tattcgcttt agatatttta ttgctgatgg tgctatggat
    13501 aattacccaa cctatgacct tttaaaatat tatgatatgc taccttttat actcatggat
    13561 tccaaaacaa aggctaaact taactattct caccctgata ttctttgttt tgatgataag
    13621 ggtcaccgta tatgcaatgg tggtattcct cttgttaact ggggttattc taatcccaaa
    13681 ggtattaaat accgttgctg gtttgctgta aaaggtttga gccacctgca gattgtaaat
    13741 gctccaatag tagttatggc aatgtagtgt atattaagcc tgactttgat ccaagaatgt
    13801 ttcctcctgt tccttgctat tctgaggcat ataaaaataa atttaaaacc agaacctctg
    13861 ttgaaaggtt taataaacga atgtttgagg attatgctct taaatattac ggttcaagaa
    13921 gctccatgat gagagcatct ttagctactt ttacagttgt taatattcat ttggatgctt
    13981 ggattaaaca catatgcttt aattttatca atataatcga aaaacaagct gcataatgca
    14041 taaaatagtg aaatagttat atcataccag cacaccttta caaggtttgt ttgctatgct
    14101 cttttttcgg ttatcctctc gtaacaatat gcggttttca aagatccatg tttaatgcta
    14161 tacaaatatc tacttatttc caaaattaat tcctgtattt tcttgttgac tccttatcaa
    14221 cagaatttcc agttactttt cgagagtctt cttgatattg gcttgcgaaa cctatatcat
    14281 aatatcacag gagtcttttt tactctaggt aacactactg tttattctat tcaccccatc
    14341 tcagatggag gattaatata ctgtttcatt aactattatt tattatacta tctttttatg
    14401 tttgtaccaa aatttaatta atttagaact cacttatttg ttcattagaa tatcatattg
    14461 atctacttgt atcttataca tctcagcata ctttccattc atgttcataa gctcgtcatg
    14521 cgaacctgat tctactaata gaccatcttc tataaaaaat attctatcca catttttcac
    14581 attagctaac ctatgagata ttaatattac agttttattg cttgcaagat caagtaccga
    14641 attgagtatt tcattttctg caattgggtc aagtgcactt gatggttcat ctagtattat
    14701 tatactacaa ttttttacat atattcttgc tatagcaagc ttttgtaact cacctccaga
    14761 gaaaattgct cctgtatttt taaattctct tgttaataca gtatacattc ccattgatat
    14821 agaattcacc ttatcatata accccacaaa cttaagagca ttatttacaa gtttctcatc
    14881 ttctgccttg ttaatcatag gacgcattaa cacattttca gcaattgtca tagcatatgt
    14941 ctgataatct tgaaaaacta ttcctatgtt gtttctcaaa gagctaacag tataattttg
    15001 atataaagta tcatttaata gtattcttcc ttctgtagga tcatatagtc gtgttatcag
    15061 tttaacaagc gagctttttc cagccccatt cctacctaca aatgcaacct tttcacctga
    15121 agatatcttt atattgatat tctttaacac gattttatct gtgtttggat aaataaaagt
    15181 tacatcttta aactcaattg actgcacgtt agtagcatct atactattgt gattttcact
    15241 aatttgcgat gtgtaattaa ttaaacctat aaaattatct atatatatgc tattctcata
    15301 cagctttgga aatatatcta gcaattgaga tatttggtga gaaagctgct gtgaactact
    15361 ccacaatgca ataaaatcag cgatacttaa gcccttatcc agaactctat atgataagta
    15421 caacataata gctgaattga ccccagaccc taaaatacta tgtgacccta ggtattttga
    15481 aagctttttg ccatatgtcc ctatgagtga aataaggttg tttgaagaag tattaaaaat
    15541 agatttaata agcgatgaaa gcttatcaaa aagacggata tcttttgcat attccctttg
    15601 ataaaacact cgttgtgcat aacctatctc tctttcaagc ggaattttct cctcataata
    15661 tttatgctgt attttaatag tttttgtact acaataaaag tatattgcta catttgtaat
    15721 aacaaacata agtataatag gatctaagca actaattaaa gtcaccaatg cacctatgcc
    15781 agataagctt ccaatcaaag atgaaaaagt acttaataca gcaactgctc tactgcctgc
    15841 ttgttgagtt gcaagagtaa atttgttaaa atattctgag tcttcgtaac attcgtaatc
    15901 cacttccatt gcctttctaa atatttccat ttgcattttt tgtcttaata tttgtgtatt
    15961 tctaggtatt attcgttgtt gtaaccaaat attaatgaat gaataggtaa tattaactat
    16021 cgttatccct agtacagata atgaaaccac cttaaaattc gtactttttg ttatcgtatt
    16081 aactacatgt ctttgaaata gaatattaaa tacagagatt atacttgcca aaattgcatt
    16141 taaaatggta ataaatatgt gacttggaca aaatctagcc acgtatttaa gcatcaacat
    16201 gttgttttgt ataacttttt tcatatttat acccatccct ttcactccgc tcaaaacaca
    16261 aaatatagat gaagaagggt tgtctttgag caattattat aagtcataga gatctcaaca
    16321 cactacaaat cacggggagg tgagtaaggc ctgacttgtt agatgacttt atatttatat
    16381 gtgtagacta tcttttcaaa tattaatgag tggtctgccc tgattagatt ggacatacaa
    16441 ttacgttagt ttactgccat tttttcaaaa ttcgtccatc tttcgttaga ccaattgttg
    16501 gtctccgaga gtacctatga cgacaagcat ccccatatga ttatggtagc taaagcaaaa
    16561 caacatatta tgatatgcgc cttcgtagga tagtggctac cgccctcgtc aaggatatca
    16621 tacggatccc tcttattgta aaggggaact tcgccaatct ctacttaatc ggtcttaagc
    16681 tgcttttccc ttattttgca gtaattattt atatattctc ttggcgattt atatcccagt
    16741 gcggaatgta aacgctttga gttataaaag cactctatat agtttattat agtctttctt
    16801 acctcttttc ttgttctaaa atggattcta tatatcaaac ctttccaaac ctttctttaa
    16861 gcttgagaaa aatgattcgg tacaagtttt attaaagcaa ttaccttttc gactcatact
    16921 ctgtacaatt tcattatact ctggaagctt tctgttcttt ttgatatact ttgttgtttt
    16981 tgttatttta ggccatagac ctatactctt ttatttgacg atgtcatgtt cttatcaccc
    17041 gacagctatc ctaacaatga ggatataaac aatttgttat aaacgccatc tatatataag
    17101 gttgatctat caagcattct attactcagc agcacttaca aacataatgt taaggtgcca
    17161 gtgctagcac ctttaaaaaa tcattggtaa tttgcggcta tgtaccataa ccagtataac
    17221 actcactaga acaatgtcca gaacaattac taccaagttg ccaaggtacc acaaccagta
    17281 tagcacccac cagaacaatg tccaaagcaa ttactaccac aatcaccgcc acatccttca
    17341 ctacacattt cagaacaagt gtagaaacat gttatagaac aatcataatt gcataatgaa
    17401 ctacaataat acggcgaaca catataatac acccctttca ttcattgtaa taacttctta
    17461 taaaaacaca cccaaactga tgcattttta cgttataatg tgcagcacta cacattaaaa
    17521 atacataact ctatcagctt agttttgttt tgccataaga agttctactt aacctctttg
    17581 aagtgtatga ctattgagct aaaacctcat tggtctcgaa atttatacgc caatttttgc
    17641 cttcccattt attcttttgg cttgttacag tccaccattg ttcaaataat ctcactgttg
    17701 ttccataatc tgaaataagt ttgttatata atttttcatt tgcagggtcg attattttca
    17761 caagattttc caaagctatt tttttttcaa ataaattttg aatctccttt ttttcatcat
    17821 cagataattt ccaaaccact ttgttcataa taatctcctt tccgctgctg tcgctggcgg
    17881 cacaaataat aatgaaaaat ggtgtgcgac ttcaccaaat ttactacaat ttattcaagg
    17941 ggaagatata ctacaaagca cggtgtaggt agtcatagat tgctcttatc gactttaaac
    18001 agtataataa gcagtgtaag aatcatcttt caagcataat taagtggttc tttaagaccg
    18061 cacgctaacc attgctttag ctactcctta aatgtgtggt gattcattca atgtcttcct
    18121 ccccaattat taaattttat tttattctaa agggggttgt gctatgaaag ttatttaccc
    18181 tatttcctgt ggtgtcgata ttcacaaaac ttttcttgtt accacactta ttacttccca
    18241 gggaattacc cctcactatc aaaaaaacgc ttttaacctt tcaataattc catatgccaa
    18301 tacaagcagt gactgattaa tagtaactgc ttcaacgtgt gtatagaagc tactggcaag
    18361 tactgggttt agtccacaaa ctattgtagg ataccatccg tattattgtt gctaatccaa
    18421 aaatgggtca aggttgtaaa aggaaataag gatggtgcaa aagtctccaa atggattggt
    18481 gacattttcc gtcttggttt agtcccaggt agctaaattc ccgataaacc aatcccgtat
    18541 tcttcgtgaa tacattcgtt accgtttcaa acttgtttct tgtaaaagca ctgaaaagaa
    18601 ccgttttcag aatgctttca ctgcttgcaa tattgctctt gacgctgtcg tttctgacat
    18661 atttggcaaa tctgcctcat ccatcacaga ctacttgatc tcgtctaatt cttttgacct
    18721 agatcattgt tctactcttc ttaaaggacc cttgaaaacg aaagtagatg aggtagttaa
    18781 atccattgga gcgtatcaga tgatcaagga gcaaaaggaa cgtatggtaa tagttcgctc
    18841 ccagcttgat tttgtcgatt attccattgc aaatctggat cagatgcttg gtaagatggt
    18901 agctccttat gggagtataa tttctctgtt ttgtactatt accgatgttg agggggcatc
    18961 tgccattacc atcatctacg agattggtac agatatgtct cagtttgcca actccaagcg
    19021 gttatgctgc tgggcgggat tatcgcctgg caacaatgga tctgtcagta agaagaagtc
    19081 tgttaaagct ttatgtgccg gagtctacct caaacctgca ttggtacaag tcgctcatgc
    19141 ttccatgaaa tccaatacat ctccttacta caagaacaaa tacgaacata tttgcaaaag
    19201 gaggggcaga aaaagagtca ttattgctat cgcaagaatg atactcaccg ccatatacca
    19261 catgtttgtt actggtgaag agtgaaatcc aagtgttctg tacaaaattg tttttccaca
    19321 agaaatacag gaaaaacaga aagaaaaagc tattaaacaa gctaaaaagc ccctgatttc
    19381 cgaaggcata ataaagatac tgacattatt atggcttaag ctaacaatta ttaatattaa
    19441 attttcaagg agcaacagta cctttattta agtacattct ttttaagtta tactgagata
    19501 tttttcacgc taatctccat tattttaagt ttacttttca aaggtaaatg aataatctaa
    19561 gatcgaagca tccttatatg gactagtaca taaccaaata tcttttaata taaaatactc
    19621 atagtaaaag tacatactac acactaaaca aatcaagata cagttagcac aaagcttaat
    19681 ggatcacata tgtattatta atacaatttc attatcacaa tcatcttctt ctattaagtt
    19741 taattatctt tgtttttctt ttgttttctc agttttgcaa attgaaaata gggagctttg
    19801 ttcgtacata tagcgcacta tacatatatt ggttctttat gagtatcaca ataaaagatg
    19861 catcatctta acatgagtca gattttttac tgtcaataac attactagga aaaataatag
    19921 atttcataaa tacttattct taaaattttt ttctttaact caagggaacg ctttttccaa
    19981 cacatttgga aaagcatctg cagcaattat taccaacttc ctctctgtgg atgagattgt
    20041 atgtattagg cttaaagagc tatcaaactt catcagtgaa aagggaaaaa actgtttccc
    20101 agatatatgt tccccctgaa gaataatgct ctgttattgc tttttcgaca tgcttaaatt
    20161 attaacttat gcatgtctat ttgagtgctg cttttcttgc ctttttcttc tataaatcag
    20221 gttttcagcg ggttattaat tccaattgag ttttgtacat ttagcacagg actatataaa
    20281 cctaaaatgg aagaatgtat gcattgatat tgatatatat gcaacctttt tcaatatttt
    20341 aagtattaat acttgtaata tcctgtcaaa gctccaacat tatcaatgta aatactcatt
    20401 ggatctttat atttaattga tgaaattata tcttcaatat tccagttagt ctccacttgg
    20461 tattgccttg agatttccct ttctatatag cttttcctgc attgatatga aggcgacaca
    20521 ttgacttcat tagtcgctga ttcattgaga tatatacaat ttatacattg atatgcatca
    20581 cataaactgc ataatgcatt gtaacttgtt ttgtacaaat gctcattttt aaacctattt
    20641 atttcttccc ctatattagc aatatgacta tcttcacgtg atgaataata agcagaacag
    20701 acataaagtc taccatcagg agcatatgag aaagaacgtt ctcccgcttt acaattatta
    20761 cgctcattta tataacataa atctgtaatt aaatttatct cctgtttgag accagtggtc
    20821 ataaaaaatt caacaagacg atttttaata gttattaatt gttttctata ttcagcctca
    20881 ttgaagttct catctaaatt tagcacattt acatttattc tctttactat gtttaacagt
    20941 tttaaaacat agtaagacag attttcaatt tctttagaat ctatattgag catacatttt
    21001 gatattcctc ttatggagag atttaaatca tcttcctcaa atacatatag acaatctctt
    21061 aattgggaag cttccttatg aaattttgca ggcactatat gtagtatgcg atattgctca
    21121 tactccttta tatttgcaaa attaaattca ttacttgaat gtacaaatat tgggctaaaa
    21181 aagttactac gtgcatattt taaacctata ataatatcat gataattcat tgtatttgct
    21241 gttacagaag tatttttgtg ttggcagtag gttatgtaat catctgaaag taaaaaatat
    21301 aagctcttat gatctctttt aagagcatcc ctttctatac catagaggtt aaataatttg
    21361 ctgaagtaat aatcattagc ccgaactctt gctttatgca ttttgcatat gtatttagct
    21421 ctactaaaat tggtgggtgt attggcttca tcgtagttga atccttgaca gaaaccacag
    21481 ccacttgcaa cttcacaatt aatacattct tcatcactct gcaatttgat catagcagtt
    21541 ataaatgggc gcaccttctc catatctatt ccatcatcaa ctgtaccaat gacccattcc
    21601 tttttattat taagagaata gtccttatat cgcaaacatg gataaatgtt tccatttgaa
    21661 ccaagagcaa tcattttacc cgctccacaa tatgttttat ttaaatcatc agagttataa
    21721 tatccaccga tattttcatc aaaaaatgta caataatatt tatcatatag cttgttatgt
    21781 aaaacataat ccgccagttc aactagttgt ttttcaaata ataaatcatc tccttcctgc
    21841 caaacgtttt caaaaacaac atttgaagca atattagtaa tcccattatt ccaaagagag
    21901 atgatactct ccttaataaa ttttagatca ctacttgcaa atgttacttt tgtactacca
    21961 ccaaattgtg aaatccagag agggatgttt tttttaataa gttcatatga accgctacca
    22021 ttaggaaata ctctatgcat atcgtgcttt tcttttgttc catctaaagt tatgctaaca
    22081 gaaagctttc ccatgttttt atttataaaa ttttgtatat gttcatctga ataatttaca
    22141 ccattagtac atatatttat ccggtaattc caataccagt catgatcctt ctcaaaagct
    22201 acaatcttaa agtaatcact gatctgatca atcaactcaa tttcaataaa tggttctcca
    22261 cctataaatt caataattac tgcttcgttt gtgcttatat tccctgcaaa aatataatta
    22321 ataaattttt tggcagtttc caaactcatt tttttatttg agcttttatg agttatatag
    22381 cagtacttgc atcgtaaatt acagtcctca gtaataataa aagttatcga ttgtgcattg
    22441 gaatctttcc acgctggaat aaactttcct accttgtatt cttttctttt tgtatcctta
    22501 aaaagtatag aataattcaa tttacaccca cctttctttt aaatacttgt ttatagatcg
    22561 ctaagctttt gcctcttgat gtgcttttta ataaaaaaac tccaaagaag tagaagctgt
    22621 tttagcaatt tactgtacag tatctagaca tattacagag catgttaatt gaaagtatta
    22681 atgctgctag tacctttgaa gaggttatct gttttagaaa gcgagtacag ctgctagttg
    22741 tttgataatc atgcaattac caaaagacca aaagaattta tgttcgtaaa atacaatatc
    22801 cataccctag caaaagctat aaacaaaaca tgacaaataa aaatcattga gattcattaa
    22861 tgatgacatc aaccttgtgt tctttaaaat ctagatattc cgacctttaa tgatgttaat
    22921 taacactttg cctgatttaa ttgattttaa tgtttgcgtg tccattgtta taaaagtaaa
    22981 cagtacaggc tatgtctcaa gtcggtagtt aagtacctct cttgttgcaa cataataatt
    23041 tccacttttt cacactgtaa actttttaat atcactagga tcatgtattt tcttagcatt
    23101 ctcctttacc cggcaggtgc ttactgaaga taatacaagg tctaaatttg gatattactt
    23161 aacagttcta taacaccacc tttgaggtat catctatcgc acaacaagat tatttgctac
    23221 tcttcaagct aaggaatcac atttttcaca agttttacag ctattccaaa tttcgtctat
    23281 tgctaatcat aaaacctata cgccacaaat ctatacagat aattcacgtt attacccata
    23341 acaaataaat attaataact tgtgatataa agtaatgcat gtccaaatgt ttttatctat
    23401 tttttagtat aaccagaagg atagataaaa caatcaagtt tttatcgaac ttaacatgta
    23461 gaacattatt atggtaaaaa atattttggc aaaagatttc agctcttctg gtatcagtta
    23521 tagcacaatc ttgactttta catgcagcaa aagttcacat aagaagcttg aatatgacaa
    23581 aaaaataata aaagtactta ggagcccctc tatagagtat ttttgatgaa ggtttcatac
    23641 aagaatttaa ttctatagac tccatctgta tacaacatgg acattatcat caagataaac
    23701 aggaattagc tccttttcca aaaacataat gaatagcaat aaatacttgg tatggttcgc
    23761 cttatcgaat taccggaaat actaatcttg gtcaggtacc tcctactggt attttatttt
    23821 gcatgattag tatttgtagg attttataca tccagccatg ttaattttat ataattttca
    23881 atgtccactt accaaaactt ataaaattgt tttactttat taataatagc ataaactaac
    23941 aaccttgtca attcttttcc agttctggca gttctgagga gtaaaaaatt atatactatt
    24001 tttgaccctt gggttggaaa ttacatttag tccaaaatat ggcatttggc atttccccgt
    24061 ttatagccaa aaatgttaaa taagatatac attttttctt tttatttaat taaacccttt
    24121 tatttgcatc acttccttgc tataacctga attctgcaca caaaaaatgg aaaaacgcct
    24181 ttgcaaagca ctaata
//
LOCUS       DAOSDD010000035        24189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_934_length_24189_cov_4.841054, whole genome shotgun sequence.
ACCESSION   DAOSDD010000035 DAOSDD010000000
VERSION     DAOSDD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24189
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_934_length_24189_cov_4.841054"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..1879
                     /locus_tag="PK733_05025"
     CDS             485..1879
                     /locus_tag="PK733_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09938.1"
                     /translation="MIWNLSRTKPALPNSYFWTWDHRTNWVLDDPGILNFGCYNKYLK
                     LPDTYISEYEKLIDFAAGLGIKGIVIWGFLRDSHGGIESAKHIASYGASKGVAILPGV
                     GTTWYGGVYYEGEHRYNLDAFLRKNPDAKMIGKDGKPYNYSDCIGACPTNPLFREWIY
                     EAVKWLFKEFDIGGVNFENGDYLLCLCPNCRKHKTGWSADDPDFFRIQALGYETVLEA
                     VEKEMKNKLITLATYTGFVPGGFKPDENTPDGVLDITHNMHCDRPAMIDHLNKDAIMQ
                     WTITGMVHKKPLPLTLFLDNGVPEEIFDNTNWPVGLNPPSVRNTGYLHQERYNHSVGI
                     IKEACLRAYRSGLEGVGIYGEVPSWHIPAALNYLAFSHFIHWPEDSLLDFGKKTLGQI
                     FNNEKEGEVFAEIFAHWDAGTITDFQNKEAKKRASELLKQVRIGKDLEKWRFWDWLTK
                     VIEGNIEQQTINIF"
     gene            2075..3841
                     /locus_tag="PK733_05030"
     CDS             2075..3841
                     /locus_tag="PK733_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ09939.1"
                     /translation="MTYLFIAFIPTIVGGILFTYYINEEVKKEYTNAVENNLIQIDYS
                     IERMLNLCRQVTKIISVDNGIIGILQSSNLADSALIKTIAYEIVPKLQGIKEQNEYIY
                     KLKVIHGNPKIPVIYDTLYYDERINSGYWSNKLKMMRNKNFNEYDEIFIETTHEEEVF
                     PDLQDTGKNKVFTVYRPIYSVYTYKVIAIVGLDILESVFLDSLKSQILNKGEVINLIN
                     RDGLVLFSNEKNVKITSTKEIEKTNNFTIMSLNGEKYYIVKRYIKNIESYIILYIPEN
                     LLSGSNKYKLLICVVLIACFITLSVVSFLMSKIIMRRLMKLTDAVNEIKLGNLKTKVN
                     IKSNDEIGKLAENFNEMVDKINELMLDIENLNKAEKDAMYKALENQIKPHFLCNALDM
                     IRMRAEIQGNYEISHAAELIASYFTYNIERRSKYVSIRDELKNVIDYIEIHNIVSNNK
                     IQYKISISSEIDGYLDEYKILKFILQPIIENSIKHGFKGRKEDSNIFINIMYVGDIVI
                     SVEDNGDGINAERMKEINACLDTNTGIVNSDTLTSNIGLRNIKERLVINYGENYGLEI
                     ESYPGIGTCVTVKTPLIKHITE"
     gene            3863..5386
                     /locus_tag="PK733_05035"
     CDS             3863..5386
                     /locus_tag="PK733_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ09940.1"
                     /translation="MHGILVVDDQSVVRQGLIKMIEDMQVEFGTIYEARDGEEALTVA
                     REQKPDIILVDIMMPGLNGIQLIEKLKEEKIKTHVVIITAYSDFEFTKKAISNKVDAY
                     ILKPVIQNELFTVLMSIKEDIEKDIKYLKVAKDKEHQYYQMMLYKYLIGDDVLINVDR
                     IFKNIYKAQCINPYYKIAIIYFSREITEEYFQVKQIIDKEIRKYGYAFVSFGIAYNKL
                     IYILNIQDSNNTNCDCIIKSCLDLVDTSVSCGISGILEGLHSLKELYHQADIAQKESM
                     FKGIKVYNFADIRNNEQLLIKINELDQILEYLSKGMKDELNNMINLVFSKVSTEGFSV
                     SDIQITLFSLINYLYLHLKDDYPGIYDIDSLKENMDNCINTFRTKVLIKEIVDGMYNY
                     VQEYRKKNSSNYAIQYIKNYVKSNFYKDVSLAKVSNELSMNYYYISDLFKKETGMSFS
                     DYVIDIRLEKAKKLLLETGIKIYEIAEKSGFYDSKHFCRTFKKLYNLTPSEYREKYR"
     gene            5699..6964
                     /locus_tag="PK733_05040"
     CDS             5699..6964
                     /locus_tag="PK733_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ09941.1"
                     /translation="MSNKKIKVGVLGAGRGMALVNVLTSHPDAELVAICDQFEPLLMQ
                     CKKISDAVDKKIELYNSYDNFLNHDMDAVVLANYANEHAPFAIELLSSGRHVLSELMA
                     VETLSQAVALVEAVEKSGKVYAYAENCCYYATILEMKRLYQQGEIGNFLHGEGEYIHD
                     CESIWPRLTRGERNHWRNRMYSSFYCSHSLGPIITITGTRPVKVVGFETPNVEYMAKL
                     GYGAGTSAMEVIQMDNNATVKSLHGLLKREPSLHWFSIYGDKGMIETDRWHEHECRIH
                     VYREDSRLADTEINYRAKYSYSTDSSEKILEHGGSDFYPIHHFLQKITGNTEKQESID
                     VYQALDMSLPGLLAYRSICNGNKPIEVPDFRKVEVRDKYRNDNWCTNDKVAGENIAPL
                     CSFGSPKLPDSIYEDVREMWVNKLKNKLF"
     gene            7106..7900
                     /locus_tag="PK733_05045"
     CDS             7106..7900
                     /locus_tag="PK733_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ09942.1"
                     /translation="MAYFMENITVKEMQEALEKTKTVIVPVGVVEQHGFHLPLSTDIH
                     NATEVPKRAGNRLDAVVAPTVPYCYSGGELAGTINISPQVFSLLMMDICAELVRIGFE
                     NIVIFLGHGGTDNKSALKMSLQMILKRNPQLRDITLSLVECYELSRTWMEYFRMEPEH
                     DFHAGAIETSLMMYWKPELVRDTIVMDEPEIARMMRTDQDWFEEVEKKVDHEYIVPIV
                     TQRNEVKVGVMGFPEKASTELGEKVSNEMVEGLIEYINLLNENTKY"
     gene            8089..9117
                     /locus_tag="PK733_05050"
     CDS             8089..9117
                     /locus_tag="PK733_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ09943.1"
                     /translation="MKYAYKGIEIHPSVEIYHDVIVFLAGITMREFFKDKAKCAQAWH
                     AGLKKIYEYFGDILPLRAVSGPPISYGHIICLGSPVTYPENSEPNVKPVLSSIDEGIE
                     LLEAKKDIDYSEQPLFKHYYEMCNYLKDQFPDQNVHFSGMGAQGPLTSAVLIRGQDFI
                     YDVYDEPEKAKRFLALLTDSIVGFHHFTNKVNGMPAINPRSAGLADDFASLISPDMWP
                     EFVVPYWNQYFEGLTTGTRTLHCENLAPAHLKYLKDIHLSHFQPSVSDLLTIENVKAN
                     TSIPFDWLLYAYNITEMSDKEIEQWVDRTVEAGITIIRTQFGQYTCQADKLDRIKAFY
                     KAFEKYRI"
     gene            9511..11274
                     /gene="abc-f"
                     /locus_tag="PK733_05055"
     CDS             9511..11274
                     /gene="abc-f"
                     /locus_tag="PK733_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HOJ09944.1"
                     /translation="MLILEAENIKKYYSDRLIIEFDNLRIYSGDKIGIVGQNGSGKTT
                     LMNILSNEIEPDEGFIKQYCDISYIRQFSDEKIETRSKNLKDSENPKALKALGEFNLN
                     DKLHQDVFSGGEKTRIKIANALSRDNLLLLADEPTANLDYKGIELLKQKLSEVDSFLL
                     ISHDRNLLDSLCNKILEVKDGKFKFYNGNYSFYKEQSEKEQQHEEQEYEKYVTEKIKL
                     EEAIIDRQNKSRTMRKAPRRMGNSEARLHTRLANEKQEKINNAVNSLKTRLEKLEVKE
                     KPRALPQIKLDFSLTNPPENKIVISAEKLTFSYNSSDSPDNCGNYHSQKIRRENSEKV
                     IFDNAKFKIYNSSKTALWGENGTGKTTLLNLISYTSINTVKSIKESIHIVPKARIGYF
                     HQGFENLDYSKSVLENVLRDSVQNQTVARTILARLLIQGNNVYKNVGILSGGERIKVS
                     FAKLFVSNANILLLDEPTNYLDMKSIEALENVLRDYEGTVVFVSHDSVFVNAVADRLL
                     IFGNKSITEFEGTLHDYMLKRQKPKTAIDSGTERIALQMRIAEIVTRLCRRDMEDGDK
                     EALEEEYSQLVLKLKKCNREQ"
     gene            complement(11563..14262)
                     /locus_tag="PK733_05060"
     CDS             complement(11563..14262)
                     /locus_tag="PK733_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOJ09945.1"
                     /translation="MLFNMIISILTSFSLVYALTSTLAFEYSPINLILVILVVLILYA
                     LIFANKYTLLLSAGLAGFSGLSWFIYAAYKKVLPVVSGKIFSIFLWLSNYVNGYEDIN
                     NKYALYITLMLIAFISLFVYIVTLKSFNFYILLGGGILLFTIQFMLDYLVSYQSFYMF
                     LFLMLIYYLKHIYLKNSKREQNNYLGNSLFTIFTLPICLVVFSIAFYIPKSSNPVEWE
                     WLDKKINSLYDYFNDKFHYISYDYFTFSSTGFGKDEGRLGGKVKLDKTPVLEVEAEDR
                     IYLRGGIRDFYTGNSWELSVNNLVEIELLDLSIGQYQYDDKSNDKSNDKSNDESNDRN
                     GDRNNRKYNEVYHSGLLEPLIGAALFKDTGYLSGQNSDKNKIPVDDFIKISKINVKYL
                     NLKTKTIFSPANFFEMSFTSGNPDGLFLDNEGVLSSERKLGKGFQYTLKNLNINYKDE
                     AFVQMLKKSYIGLYKDILNRNVKNKANTYSLYRDGIISPEGKDINISSEDIEKLAVAA
                     EQLNTRYLQLPDTVPERVKELAISIASNENNNYDKVKAIEVYLASNYPYTLKPRPTPN
                     GRDFTDYFLFVIKEGYCVYYATAMTVMLRSIGIPARYVEGYMLPPAASSDNKTVGSSI
                     TETSNTSDVPVNSNNSKGTEKIYNVTNENAHAWVEVYFEGFGWIPFEPTSPFLSSFYS
                     SQSTGTISSSFAEDPFYIDYLRMLEMYDSEGYTDLLNPNIDADSINTEIEVLSYKEIF
                     SFLIAVMLILFAAIVSVNLFRNKNKLAKIRKMDPGDSIISMAAYYLKVYEVQGTPILK
                     NETPAEFVRRIEEQSAYEIYKYENENFDNFKSRVSDTGSLGKYSGFKSVMDIFILARY
                     SSVQIKESQKDIVIGYYKRIIAESRDNLGLIKYFGYRYLLGRF"
     gene            complement(14269..15468)
                     /locus_tag="PK733_05065"
     CDS             complement(14269..15468)
                     /locus_tag="PK733_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ09946.1"
                     /translation="MLANRVLYASLLVLSITFTYFYGGIVPYMLFFCIILLPLVSFLY
                     TLVIFFRFKFHQEIDRKFIIKGDKINYIFTISNEDYVFYPYMSVTFFGSQTIFSRQFE
                     TINLSLLPRSDRIFTFELFCKYSGNYDIGVERIEIEDFLGIFKFVYKVPFPISLTVYP
                     RVIILERFRLKTDFTSESFSALQNKFEDIGALQDVRKYVYGDNLKKVHWKLTAKLNEI
                     MIKRFQSTTESSAIIIPDLKRNNFTIEQNTIVQDKIIESTISVVHYCLHKWMPVNLVC
                     YSNEVRDITASNPLHFQDIYSYISEIHFSNAINLSNISHIYIKDKTIKTNLILCTANL
                     DYDLYNHIYYAALSGFNVIVIYISSEELTGSRNDMAESIISDIPGIGANIYKININDE
                     IKTVLEV"
     gene            complement(15610..16545)
                     /locus_tag="PK733_05070"
     CDS             complement(15610..16545)
                     /locus_tag="PK733_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ09947.1"
                     /translation="MDNTLQTFKLIVENVEKVILGKREAIELTLIALACSGHVLIEDV
                     PGVGKTSLVSSIAKSIDCSFKRIQFTPDILPSDITGFSIYNQKTGNFEYKPGSVMSNI
                     ILADEINRTSPKTQASLLEVMEENQVTVDGITYNVPRPFMVLATQNPVEYLGTYPLPE
                     AQLDRFVLKISLGYPSPADESLILERFKMENPLLALEPVTDGNYIISLQQKIRSIYVD
                     KSLNRYIVEIVDSTRKHHDVVLGASPRGSLNLFRASQAWAFYNGREYVLPDDVKKMAL
                     PVLSHRIFLKQEAKLKKISPEDILRSIIGNVNVPR"
     gene            16926..19235
                     /locus_tag="PK733_05075"
     CDS             16926..19235
                     /locus_tag="PK733_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ09948.1"
                     /translation="MFSSLKNHRVLSFLLYQCLVSMILVLACTALSSAISYDVLRKKL
                     SAWSIRTNNELIEHYNDVFKVSIVTGSEKIYDQTLLALYSNSNMKYFLNNPIEKNFLG
                     INSINTYFRNIVKANPLIREVGIFYVTNHLIVTNDTVKYDYFYNFHNHEIAFFISKIE
                     EILDKGDVSNFNAYATDGAIQMIRPLVTANKVIALIFIEYSTDALNEKLKENVTDMFG
                     NLLVLDKQGTVIYDNSSGYSGKHYSQLPIYSDKLNNADRGYYFKNIDKVRSIISHMSD
                     NNSWRYISITPEQFYMEPVQYILKNLIICILVSLLASIIFIVTMSLWQSRSMRSIIEL
                     CDKTGENNSIPSKFSDIYGFIRSTLTELMRTVETQTQEIKKLMPILRKNFVLWLMSEK
                     EADAGEVNDTMLLMRVEFPFENFAFLAVKAVYEYINIDAESDEFDPGYALAEASVILK
                     AAFNTNDSIGVFYRNSDYITGLINFSFDSKTLDRRCNTLFKQSFHGFRFYIASGPVAS
                     NISELAGMANSTIESLRYSYLFPENKYFSHEFVSRFGKVELNISNELSMFSEAMKRKD
                     SKCTIELLYTIVQKLLHSGCTPQYAQQVMLEASSIIEKFSRPGINEELIHIFGACNDI
                     LSLYQALRDIISLEFDFIPIVSNNTLVLINDAKKYIDRNLTSSQLSLQSVSDELNITT
                     QYLSQIFHEVSGITFTEYINSKKMKLACQLLNETNLNITDISEKLCYSSPQYFIRRFK
                     KFYGVTPSSYRHNMKGRLTEEVSEISLLH"
     gene            19481..21127
                     /locus_tag="PK733_05080"
     CDS             19481..21127
                     /locus_tag="PK733_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09949.1"
                     /translation="MKRKLIMTVIYVLLIVFVLGACTTGANKTVATDKTGKNDTTSKT
                     EATASAQKEPPAELSWFFHPQGTTWQEFDFSNNWFIDIIEEIANVKFTSVITPPHADV
                     WTKFGMHIASGELMDLMYGGASMADLNKYGKDGAFLQVDNYIDNSPIISKVYNSAQRE
                     YIKAEDGKTYVIWGYPGNADFGTNEGFGYRADLLEKVGLDTPTTLDGWVDAMRKVKVE
                     IPNTIPYSTVNLAQYYELMFSPFNIMFNYGTWIYNDESHTYTHAFDTENMVKALTFGR
                     MLFKEGLLDNEFMTHTKSDYTKKIYSNNMFIVVKNRGGISGFITKYPANGVQGAKLLP
                     SWYPAADGIDMKDGVYKHWGQLIGAPMVISSKTKSADACIRVIETLLSDKVRNLVVYG
                     REGYEYEVVNGVKTPIQPAETDNGWRIGYGLMVGVNTAQRMSYKEDAAIDASPSYASE
                     EKEAYKKAFSERAAKFTELVSIDTPRSNLYAAPLSDELSIKRSDSFELQKSLIAKTIM
                     GDISIDEFRAEAKKVVANDADIVAAMNKLMSEAVKKYGLK"
     gene            21213..22160
                     /locus_tag="PK733_05085"
     CDS             21213..22160
                     /locus_tag="PK733_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09950.1"
                     /translation="MNKEISQTTLKSKKPDILIRFRKSIPVYMLILPSTALLLIFHYL
                     PIFGISIAFQDYSVYKGVFGSTWVGIKYFERFLTDANFWRVMRNTIIINVYDLIFGFP
                     VPIIFALFLNELRSNKLKRVAQTISYLPHFISWVVVAGMVVTILSPSTGLINIMLKNI
                     FNTEPIHFLAKPQYFRSILVTADIWKHFGMSAVYYIAALSSIDMELYAAASIDGAGRL
                     RQTWHITLPGLRLIIIVLLVLKMGKMINIGFEKVFLLYNPILYEVGDVISTYTYRLGI
                     EKNQYSLTSAIGLTQSVINFILVFSANKLSRKIAGWSLW"
     gene            22179..23051
                     /locus_tag="PK733_05090"
     CDS             22179..23051
                     /locus_tag="PK733_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ09951.1"
                     /translation="MKQTRGEKIFQCFDIFIILLIITAVIFPLLNIFAISVSDASSIV
                     NGKVALWPKGFNTAAYSKILSNNHFIRAMLNTILITVTSTFLSVLLSLMTAYGFTKEF
                     LGKKFITYLYVLTMYFSGGLIPTYVVYTKYLHLRNNFLVLILPGLVSMFYIIIVRSQI
                     ESLPLSVLESAYIDGATEFQTAFKIVLPMISPTLAAIAMFTALGNWNIWFQVMIYTDY
                     PKFWTLQYYLRTIVFGQFLAANEFITEAAGEFIPEENYRMAAIIIVAAPIVCIYPFVQ
                     KYFVKGIISGSVKG"
     gene            23114..23929
                     /locus_tag="PK733_05095"
     CDS             23114..23929
                     /locus_tag="PK733_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ09952.1"
                     /translation="MQWEHLVPSDFRKAVNETRLCILPTGSLERHGEHLPFGCDMIVA
                     HTIACKAAEIEPAVVFPPYYIMQVHEAACFTGTVNYPQAFALEALRLLLESIAANGFR
                     KILVVNGHGGNKHMLDYFAMSRVDELTEYIFYQTGIYGKLTQEEDNIIKNVFGTNSGG
                     HAGELETSLFMACCPGKVKLEYAQQKPVMPLKRFKHLWDNGVNNALWWYADYPENVAG
                     DARLASEEKGRVVLDVYAKSLARAIKTVKEDTEAVRLQTEFLKYVKNKGNYGG"
     gene            23935..>24189
                     /locus_tag="PK733_05100"
     CDS             23935..>24189
                     /locus_tag="PK733_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ09953.1"
                     /translation="MELLNNKLNWYKGNLHCHTNISDGLISPSECIDAYKKAGYDFLS
                     ITDHAKYYKGYETENFVLFSGTEFHHVNWVTRKNFHILGIG"
BASE COUNT         8318 a         3814 c         4519 g         7538 t
ORIGIN      
        1 tggaagatat tacaaaaagg attcctgtta caaccaattc actattgtta gccatgatgg
       61 tgataactgc ttttcctata attgtattat acccgtttct tcagaagtat tttattaaag
      121 gtataatgat tggctctata aagggctaag tgcgacacga ggttgcacag ataaacattt
      181 gaaaggcttg atacgactat gtttcatagt atataacaaa cctcttgttc cgtcaagagt
      241 aatctagcaa taaatgcgga ggtggtaaca ttttaatcac aggtcagaga tagtcacagg
      301 atacacatgg cacacatgtg gtatagaggt gaaatgggac gcgagaacac actagggctg
      361 tgtcatatgt gcctaaacca tctccatgca tgcactctgt tctgtacctc tgacctgtaa
      421 ctggtagtct gttttgaaaa tcgctaagca aatctgaaat caataaagta tggaggtagc
      481 attaatgata tggaatttat caagaactaa accggcttta ccgaactcat atttctggac
      541 atgggatcat aggacaaatt gggttttaga tgatcctgga attctaaatt ttggctgcta
      601 taataagtat ttgaaattgc ctgatacata tatttcagaa tatgagaagc ttatagattt
      661 tgctgcaggt cttggaataa aaggtatagt aatatggggc ttcttaaggg attcgcatgg
      721 tggtattgaa tcagcaaagc atattgccag ctatggagct tcaaaaggag ttgcaatttt
      781 accgggtgtt ggaacaacat ggtatggggg agtttactat gaaggagagc atcgctataa
      841 tctggatgct tttttaagaa aaaatccaga cgcaaaaatg ataggtaagg atggaaaacc
      901 atataattat tcggattgca ttggagcatg tcctacaaat ccattgtttc gtgaatggat
      961 atacgaagct gtcaagtggt tatttaaaga attcgacatc ggaggtgtga attttgagaa
     1021 tggagattat ttgctttgcc tttgcccgaa ctgcagaaaa cacaaaactg gttggtcggc
     1081 agatgatcct gactttttcc gcattcaggc tttaggatat gaaactgtac ttgaagcagt
     1141 agagaaggaa atgaaaaaca agcttatcac gctagcaact tatacagggt ttgtacccgg
     1201 aggatttaag cctgatgaga atactccgga tggtgtgctt gatataacac ataatatgca
     1261 ctgtgatcgg cctgctatga ttgatcatct aaataaagat gctataatgc aatggactat
     1321 taccggtatg gttcataaaa aaccattacc cctgacttta tttttggaca atggtgtgcc
     1381 agaagagatt tttgataaca ccaactggcc tgtaggtctg aatcctccat ctgtaagaaa
     1441 tactgggtat ctgcatcagg agcggtacaa ccactctgta gggattatca aagaagcatg
     1501 ccttcgcgct tatagatcag gtttggaagg tgtgggtata tacggggaag taccttcatg
     1561 gcatattcct gctgcattaa attaccttgc tttttctcat tttattcatt ggcctgaaga
     1621 ttctcttcta gactttggga aaaaaactct tgggcaaatt tttaacaacg aaaaggaagg
     1681 tgaagttttt gctgaaatat ttgcgcattg ggatgcagga actattaccg attttcaaaa
     1741 taaggaagct aagaaaagag cttctgaatt actaaaacaa gttcgtatcg ggaaagattt
     1801 agaaaaatgg cggttctggg attggttaac aaaagtgata gaagggaata tagagcaaca
     1861 aacaataaac atattttaat ttattacaga ttattataaa aatatttttg aaagacaact
     1921 cgaattttat catataacat gataaaattg atatacagat ataatgcaat taattcttat
     1981 ataataaact tattttcgag gtttttaaga agggagtgat atagaaataa tattatataa
     2041 agctgtaagt tcaagtatta caaaaaagtt aatattgact taccttttta ttgcttttat
     2101 ccctactata gttggaggaa ttttattcac ttattacata aatgaagaag ttaagaaaga
     2161 atatacaaat gccgttgaaa ataatctcat acaaatagat tacagtatag aaagaatgct
     2221 caatttatgc agacaggtaa ctaaaataat tagtgttgat aatggtatta taggtatact
     2281 gcaatcaagt aatcttgcag attctgcttt gattaaaact attgcttacg aaatagttcc
     2341 caaactacaa ggcataaaag aacagaatga atacatttat aaattgaaag taatacatgg
     2401 taaccctaaa atacctgtta tttatgacac attgtattat gatgaaagaa ttaacagtgg
     2461 atattggagt aataaattga aaatgatgag aaataaaaac ttcaatgaat acgacgaaat
     2521 atttatagaa acaacacatg aagaagaagt ttttcctgat ttacaggata ctggtaaaaa
     2581 caaagtattt actgtgtaca ggcctatata ttctgtttat acatataaag taattgcaat
     2641 tgtaggactg gatattctcg aaagtgtttt tttggattca ttaaaatctc aaatattaaa
     2701 taaaggtgaa gtcataaatc ttattaacag agatggactg gttttattca gtaatgagaa
     2761 aaatgtaaaa ataacatcta ctaaagaaat tgagaaaacc aataacttta caattatgag
     2821 cttaaatgga gaaaaatatt atattgttaa aaggtatata aaaaatatcg aaagctatat
     2881 aatattatat atccctgaaa acttactaag cggaagtaat aaatataaat tattgatttg
     2941 tgttgtactg attgcttgtt tcataacatt gagtgtagta tcttttttaa tgtcaaagat
     3001 aataatgaga agattgatga agcttactga tgcagttaat gaaattaaac tgggaaatct
     3061 taagacaaag gtaaatatta aaagtaatga tgagatagga aaactagctg aaaattttaa
     3121 tgaaatggtc gataaaataa atgagttaat gcttgacata gaaaatctaa acaaagcaga
     3181 aaaagatgca atgtataaag ctctcgaaaa tcagattaaa cctcacttcc tatgtaatgc
     3241 acttgatatg ataagaatga gagcagaaat tcagggcaat tatgagatat cacatgcagc
     3301 ggaattaata gccagttatt ttacatataa tattgaaaga agatcaaaat atgttagcat
     3361 tagagatgag ctaaaaaatg ttatagatta tattgaaata cataatattg tcagtaataa
     3421 caagatacaa tataaaattt ccataagctc tgaaatagac ggataccttg atgaatataa
     3481 gatattaaaa tttattcttc agccaattat agaaaactca ataaaacatg gcttcaaagg
     3541 aaggaaagaa gacagtaata ttttcattaa tattatgtat gttggtgata tagttattag
     3601 tgttgaagat aatggtgatg gaattaatgc agagagaatg aaagaaataa atgcgtgtct
     3661 tgatacaaat actggtattg taaactcaga tactctaacc agtaatatag gcttaagaaa
     3721 tattaaagaa agattagtca tcaattatgg agaaaattat ggtcttgaaa tcgaaagtta
     3781 tcccggaata ggaacatgcg taaccgtaaa aaccccgctt ataaaacata taactgaata
     3841 atcttataca aggagtgagg atatgcatgg tattttagtt gtggatgatc aaagtgtggt
     3901 aagacagggt ttaattaaaa tgatagaaga tatgcaggta gaatttggta ctatttacga
     3961 agcaagagat ggagaagaag cgttaactgt tgcaagggaa caaaagcctg atataatttt
     4021 agttgacatt atgatgcccg ggttaaatgg tattcagctt attgagaagc taaaagaaga
     4081 aaaaataaaa acacatgtgg ttattattac tgcatatagt gattttgaat ttactaaaaa
     4141 ggctattagt aataaagtgg atgcatatat tttaaaacct gtcatacaaa atgaattatt
     4201 cacagtttta atgtcgataa aagaggacat tgaaaaggat ataaaatact taaaagttgc
     4261 aaaagataaa gagcatcaat attatcagat gatgctttac aaatacctaa taggtgatga
     4321 cgtactaatt aatgtcgata gaatttttaa gaatatatat aaagctcaat gtattaaccc
     4381 ttattacaaa attgctataa tctattttag cagagagata actgaggaat atttccaagt
     4441 taaacagatt atagacaaag aaattcgtaa atatggctat gcttttgtct cttttggcat
     4501 tgcatataat aagttgattt atattctaaa cattcaggat agtaataata caaattgtga
     4561 ttgtataatt aaaagctgct tagaccttgt ggatacatcc gtaagctgcg gtataagtgg
     4621 tattttagaa ggattgcatt cattaaaaga gctttatcac caggcagata ttgcgcaaaa
     4681 ggaaagcatg tttaagggta taaaagtata taattttgct gatataagaa ataatgaaca
     4741 gctattaata aagattaatg aacttgacca gattctggaa tatttgagta aaggaatgaa
     4801 agatgagctt aacaatatga taaatttagt attttcaaaa gtgtcaacag aaggatttag
     4861 tgtaagtgat atacaaataa ccttatttag tttgattaat tatctttatt tgcatctaaa
     4921 agatgattat cccggtattt atgatataga ttctttgaaa gaaaatatgg acaattgcat
     4981 aaatactttt agaacaaaag tgctaataaa agaaatcgtt gatgggatgt ataattatgt
     5041 tcaggagtac agaaaaaaga acagttcgaa ttatgcaata caatacatta aaaattatgt
     5101 taaaagtaat ttctacaaag atgtttcgct tgccaaggta tcaaatgaat tatctatgaa
     5161 ttattattat attagtgatc tctttaaaaa ggaaactgga atgtcatttt ctgattatgt
     5221 tatagatatc aggcttgaaa aagcaaaaaa attattatta gaaactggta taaaaatata
     5281 tgaaatagca gaaaaatctg gattttatga ttcaaaacat ttttgcagaa catttaaaaa
     5341 attatataat ttaactccta gtgaatatcg ggaaaaatat aggtagttta ggtttaaatg
     5401 ctctgaaaat ccagtattgt atccatagtt tccagcaaaa tatccatatt gtaaagtcgg
     5461 actttattgc actatataat ttacgatttg aaataatcaa tgaagaatta gcaaatcgta
     5521 agttacatca aggtaacaga agcaggtttt aaacatttta aaacctgttc tatataggca
     5581 tcaatatata taaagtgaac ccaattttcg gtaagtaagg tatagtaagc cgtttagaaa
     5641 taaaggttcc aagacttctc aaaaactaaa aatattattt tcgaggtgat ttgttattat
     5701 gagtaacaaa aaaatcaaag ttggggtttt aggagcaggc agaggaatgg ctcttgtcaa
     5761 cgtgttgacc agccatcctg atgcggaact tgttgccatt tgtgatcaat ttgaacctct
     5821 tttaatgcaa tgtaaaaaga tatctgatgc agtagataaa aaaatagaat tatataattc
     5881 ttatgacaac tttctgaatc atgatatgga tgcagtagtt ctcgcaaatt atgcaaatga
     5941 acacgctcca tttgcaatcg aactgcttag ttcaggcaga catgttctaa gtgaactgat
     6001 ggctgttgaa acactttcgc aggcagttgc acttgttgaa gcagtagaaa agagtgggaa
     6061 agtttatgct tatgcagaaa actgctgcta ttatgcaaca atactagaaa tgaaacgtct
     6121 ttatcaacag ggtgaaatag gtaattttct ccatggggag ggggaatata ttcatgattg
     6181 tgagtcaatt tggcctagat taacacgtgg agaaaggaat cattggagaa acagaatgta
     6241 ctccagtttt tactgcagtc acagtttagg acctattatt acgattactg gaacgcgacc
     6301 tgtaaaggta gtgggttttg aaacgcctaa tgttgaatat atggctaaat taggttatgg
     6361 tgcagggact tctgctatgg aggtaatcca aatggataat aacgcaactg taaaaagttt
     6421 gcatggactt ttaaaaagag aacccagttt gcattggttt tcaatttatg gcgacaaagg
     6481 aatgatagaa acagatagat ggcacgaaca tgagtgtagg attcatgtat acagggaaga
     6541 tagcagatta gctgatacag aaataaacta cagagcaaaa tacagttata gtacggattc
     6601 atctgaaaaa atattggaac atggtggaag tgatttttat ccaatccatc attttcttca
     6661 aaaaattaca ggcaatactg aaaaacaaga aagtatagat gtatatcagg cactagatat
     6721 gagtttaccg gggctgcttg cttatcgttc aatttgtaat ggaaacaaac ctattgaggt
     6781 acctgatttt agaaaggtag aagtcagaga taagtaccgt aatgataatt ggtgtacaaa
     6841 tgataaagta gcaggtgaaa atattgcgcc tttatgttct tttgggagtc ctaaacttcc
     6901 tgatagtata tatgaagatg taagggagat gtgggtcaac aaattaaaaa ataagctgtt
     6961 ctagaatatt actatactac tatttattat ctcaaacttc gtatataata aatttcagag
     7021 ggaaaaatta tccgatctta aggtataaaa aacaggtaag cataaggtaa tataaaattt
     7081 aaaacttgaa aggaggatag ataatatggc atattttatg gaaaatatta ctgttaaaga
     7141 aatgcaggaa gctttggaaa aaaccaaaac tgttattgtg cctgtaggag tagttgagca
     7201 acatggtttt catcttccat taagcacaga catacataat gcaacagagg tgcctaaacg
     7261 tgcaggaaat agattagatg cagttgtcgc acctacagtt ccatattgtt actcaggtgg
     7321 agaattggca ggtacaatca atatcagccc ccaggtattt tcacttctta tgatggatat
     7381 atgtgcagag ttggtcagga tagggtttga aaatattgta attttcttgg gtcatggagg
     7441 tacagataat aaaagtgcat taaaaatgtc ccttcagatg attttaaaga ggaatccaca
     7501 actcagagat ataaccctgt cccttgtaga atgttatgaa ctttccagga cctggatgga
     7561 gtattttaga atggagcctg agcatgattt ccatgcaggt gctattgaaa cttcacttat
     7621 gatgtattgg aaacctgaac ttgtgaggga tactatagtt atggatgaac cggaaatagc
     7681 acgaatgatg aggacagatc aggactggtt tgaagaagtt gaaaagaaag tggatcatga
     7741 atatattgtt cctatagtta cacaaaggaa tgaagttaaa gttggagtta tgggattccc
     7801 tgaaaaggca agtacggaac taggtgaaaa agtaagcaat gaaatggttg aaggtcttat
     7861 tgaatatatt aacttactta atgaaaatac aaagtactga aaaaatttat atttataatg
     7921 cagaactacc cttgttaatt ctggtcaatt aaaaagttaa attccaccct ttgatttgca
     7981 aaactaactt tcgctatttt aatgattagg gtgggtgttt atctctatgc tgagatttat
     8041 gatatagagg aattggcaag atttgtttaa taagaaggga gcactaatat gaaatatgca
     8101 tataaaggca tagaaatcca tccgtcggtt gagatatacc acgacgtcat tgtgtttctt
     8161 gctggaataa caatgagaga gttctttaaa gacaaggcta aatgtgctca agcatggcat
     8221 gcgggattaa agaagattta tgagtatttt ggagatatac tgcctcttcg tgctgtgtca
     8281 ggaccaccta tttcctatgg acacattatc tgtctcggtt ctccggtaac atatcctgaa
     8341 aattccgagc ctaatgttaa acctgtgctt tcaagcattg acgaaggcat tgaactgctg
     8401 gaggcaaaga aggatatcga ctatagtgag caaccgctct ttaaacatta ctatgagatg
     8461 tgcaattatc taaaagatca attccctgac caaaatgtgc atttttccgg catgggtgcc
     8521 cagggtcctt taacttccgc tgtacttatt cgcggacagg attttattta tgatgtgtat
     8581 gacgagccgg aaaaggcaaa gagatttctt gctctcctaa cagacagcat tgttggtttt
     8641 caccatttta ctaacaaggt aaatggaatg cctgcgatca acccgcgtag cgcaggactt
     8701 gcggatgact ttgccagcct tatttcgccg gatatgtggc ccgaattcgt tgtaccatac
     8761 tggaaccagt attttgaagg gcttactaca ggaacacgaa ctttacattg tgaaaatctt
     8821 gcacctgcac accttaaata ccttaaggac atccatcttt ctcatttcca accttcagtc
     8881 tccgatctgc tgaccattga aaatgtaaag gcaaacacca gcattccttt tgactggctt
     8941 ctatatgcct ataatatcac tgagatgagc gataaggaaa tagaacagtg ggttgatagg
     9001 acagtagaag caggcattac aattattcgt acccagtttg gacagtacac ctgtcaggct
     9061 gataaactgg atcgtattaa agccttctat aaagcttttg agaagtacag aatataacga
     9121 ggcgaaagtt gatataacgt tttgtaaatt gcttgattta caagttttca tagtgtaaaa
     9181 tataaataaa gtttaagaaa gggcaaggta ttttctgatg agaattcgct aattcggcaa
     9241 aaaaattaga tttttaaata tgagagcata tattgctctt aaatgtgctg gattagcttc
     9301 agagtaagac aggtagcttc aggataaaac aggtattaga cttgcgaata agacccggga
     9361 ataaacctga taacagaaca actgcaatta gacgttaata acctttaaat agcttttttt
     9421 acctgacagg cagaaagttt tgttgtattt atatttgtgt ttattatgtc tttccagcac
     9481 actttttaaa gtgagaggag agattttttt atgcttattt tagaggctga aaatataaaa
     9541 aaatattaca gtgatagatt aatcatagaa tttgataatt taaggatata ttcaggagat
     9601 aaaattggta ttgttgggca aaacggttcg ggaaagacaa cacttatgaa tattttgtct
     9661 aatgaaatcg aacctgatga aggatttata aaacaatact gtgatatatc atatattcgt
     9721 cagttttctg acgagaaaat agaaaccagg tcaaaaaatt taaaagattc agagaatcca
     9781 aaggcattga aagctttagg agaattcaat ttaaatgata aattgcacca ggatgtattc
     9841 agtggcggtg agaaaacgag aattaaaatt gctaatgcac ttagcaggga taatctgctt
     9901 ctattagctg atgaacctac agccaacctt gattataaag ggatagaact cttgaaacag
     9961 aagctttctg aggtggattc ctttttactt atcagccatg ataggaatct actagacagc
    10021 ctgtgcaata aaatccttga agtaaaggat gggaaattta agttttataa tggcaattat
    10081 tctttttata aagagcagag tgaaaaggaa cagcaacatg aagaacagga atatgaaaaa
    10141 tatgttacag aaaaaataaa acttgaagaa gcaataatag atagacaaaa taaatccaga
    10201 accatgagga aggctcccag aagaatggga aactctgaag ccaggcttca tacgcgatta
    10261 gctaatgaga agcaggagaa aattaataat gccgttaaca gcttgaaaac ccggcttgaa
    10321 aagcttgagg taaaagaaaa acccagagcg cttccacaaa taaagcttga tttttctcta
    10381 acaaatcccc ctgagaataa aattgttatt tctgccgaaa agcttacctt cagttataac
    10441 agttctgaca gtcctgataa ttgtggcaat tatcacagcc aaaagatacg tagggaaaat
    10501 agcgaaaagg ttatatttga taatgctaaa tttaaaattt ataatagctc gaaaactgct
    10561 ctttggggtg agaatggaac aggaaaaact acactgctga atctgatcag ctatacaagc
    10621 ataaatacag ttaagagtat aaaggaaagc atacacattg ttcctaaagc tagaataggt
    10681 tattttcatc aaggttttga aaatcttgat tatagtaaat ctgtattgga aaatgttttg
    10741 agggatagtg ttcaaaacca aactgttgcg cgtacaatcc tggcaagatt gctcattcaa
    10801 ggaaataatg tttataaaaa tgttggcata ttaagtggtg gtgaaaggat aaaagtttcc
    10861 tttgcaaaac tttttgtatc aaacgcgaac atactgcttt tggacgaacc tacaaactat
    10921 ctggatatga agtctataga agctctggaa aatgtacttc gtgattatga agggactgtt
    10981 gtatttgttt cccatgatag cgtatttgta aacgcagttg ccgatagatt gcttattttt
    11041 ggaaacaaat ctataacgga atttgaagga acacttcatg attatatgtt gaaaaggcag
    11101 aaaccaaaaa cagcaattga cagcggaact gagcgaattg ctctgcaaat gagaattgcc
    11161 gagatagtaa caaggctttg caggcgtgat atggaagatg gagataagga agctttggag
    11221 gaagaataca gccagttggt tttgaaacta aagaaatgca atagagagca atagagagaa
    11281 actttaacta tagctatttt gtgacttgag atgacaagtt cattagtgct acaataaaat
    11341 tgtacatgat accatgtaca tcgcagcatc cggagctgac tttcgtaaag aaaggagtga
    11401 tcagaagtga agattgcaat ggaggtggcc atactttaat ggccggggtg tgtcaggctg
    11461 aaaatgcctg gccgcctctt ttttatcgac catagggaca gttttcttgt ttcatattga
    11521 cgaaataaga gaaccgtccc tgtgttctat gttccgattt attcaaaacc ttcccagcaa
    11581 atatctgtat ccaaaatact ttattaaccc caggttatcc ctcgactcgg caattatcct
    11641 tttataataa cctataacaa tgtctttttg tgactctttt atttgcacgg aactgtacct
    11701 tgcaagaatg aaaatatcca taactgactt aaaacctgaa tatttcccta gtgaacctgt
    11761 gtcagatacg cggcttttaa aattatcaaa gttttcattt tcatatttat atatttcata
    11821 ggcagactgc tcttcaatac gccttacaaa ttctgcaggt gtttcgtttt taagtattgg
    11881 agtaccttga acttcatata cctttaaata atatgctgcc atcgatatta tactatcccc
    11941 aggatccatt tttctgattt tcgcaagttt gtttttattt ctaaaaaggt tgacggatac
    12001 tattgcagca aacaaaatca gcataacagc aattaggaat gaaaaaatct ctttatagct
    12061 taatacttct atttctgtat ttatagaatc agcatcaata ttaggattta ataaatctgt
    12121 atagccctcc gaatcataca tttccaacat tctcaaataa tcaatataaa aaggatcctc
    12181 ggcaaaactg ctgcttattg tacctgttga ttggctgctg taaaaactgg acaggaatgg
    12241 tgaggtaggt tcaaaaggta tccacccaaa gccttcaaaa tatacctcca cccaggcgtg
    12301 ggcattttcg ttggtaacat tgtaaatttt ttccgtacct tttgaattat ttgaattgac
    12361 cgggacatct gaagtatttg atgtttctgt aatacttgaa ccaactgttt tattatctga
    12421 agaagcagca gggggcagca tatagccttc tacatatctt gcagggattc caatgcttct
    12481 aagcatgaca gtcatagcag tagcatagta cacacaataa ccttccttta taacaaagag
    12541 aaaatagtcg gtaaaatccc ttccattggg cgttggacgc ggttttaatg tgtaggggta
    12601 attgctggca agataaactt ctattgcttt tactttatca taattgttat tctcgttaga
    12661 agctattgat attgctagtt cctttactct ctcaggaact gtatcaggca gctgcagata
    12721 tcttgtattg agttgttctg ctgcaacagc cagcttttct atgtcttccg aactgatatt
    12781 aatatctttt ccttcaggac ttataatccc atctctgtat agagagtaag tatttgcttt
    12841 atttttaaca tttctattga gaatatcttt gtaaagccca atgtagctct ttttaagcat
    12901 ttgaacaaag gcttcgtctt tatagtttat atttaaattt tttaaagtat attgaaatcc
    12961 tttgccaagt ttcctttcac ttgatagaac cccctcatta tcaaggaata gtccatcggg
    13021 attcccagag gtaaatgaca tttcaaagaa atttgcagga gaaaatatag ttttagtttt
    13081 aagattcaag tattttacat taattttgct aattttaatg aaatcatcaa caggtatttt
    13141 atttttatcg gagttctgtc cagataaata accggtgtct ttaaacaaag ctgcgccaat
    13201 aagcggctca agtaatccgc tgtgatatac ttcattatac tttctattat tcctatcgcc
    13261 atttctatca ttgctttcat cattactttt atcattactt ttatcattac ttttatcatc
    13321 atactgatat tgcccgatac tcagatccaa aagctcaatt tcaacaagat tatttacact
    13381 taattcccat gaattacctg tgtaaaaatc tcttattcca cctcttaaat atatgcggtc
    13441 ttctgcctca acttccagca caggcgtttt atctaattta actttccctc caagccttcc
    13501 ttcatctttt ccaaatcctg tagaggaaaa agtaaagtaa tcatatgaaa tataatgaaa
    13561 tttatcattg aaatagtcat agagtgaatt gattttctta tcaagccatt cccattcaac
    13621 aggattactg cttttaggga tatagaaagc aattgaaaac acgactaaac atataggcag
    13681 ggtaaatatg gtaaacaaac tgttccctaa ataattattt tgttcccttt ttgaattctt
    13741 taaatatata tgtttaaggt agtatataag catcaaaaac aggaacatat aaaaagattg
    13801 gtagcttacc agatagtcca acataaattg tattgtaaag agcagaattc ccccacccag
    13861 cagaatgtag aaattaaagc ttttaagagt aacaatatat acgaatagtg atataaaagc
    13921 tattaacata agagtaatat aaagggcata tttgttgtta atatcctcat agccattaac
    13981 atagttggat aaccataaaa aaatgctaaa aattttaccg gacacaactg gaagaacttt
    14041 tttatatgca gcataaataa accatgaaag cccactgaaa cctgctaagc cggcagataa
    14101 caacaaggtg tatttattgg caaatattaa tgcataaagg attaaaacga ctaaaataac
    14161 taatattaaa tttattggag aatattcaaa agcaagggta gaggttagtg cataaaccag
    14221 gctgaagctg gtaagtattg atataatcat attaaataac atagttgttt atacctccag
    14281 aacagtttta atctcatcgt ttatattaat tttgtatata tttgcgccaa ttccaggtat
    14341 atcagaaata attgactctg ccatatcatt cctgctgccg gtaagttcct cggatgaaat
    14401 ataaatgact atcacattaa agcctgacaa agcagcatag tatatatgat tatacaggtc
    14461 ataatccaaa tttgcagtac ataatatcaa attagtctta atagttttgt cttttatata
    14521 aatatgagag atattagata aattaattgc attggaaaaa tggatttctg aaatgtagct
    14581 atatatatcc tgaaaatgca gaggattgct tgcagttata tccctgactt catttgaata
    14641 acaaaccaag ttgacaggca tccatttgtg caggcaataa tgaactacag atattgtact
    14701 ttcaatgatt ttatcttgta ctattgtatt ttgctcaata gtaaaattat tccttttaag
    14761 atctggtata attatagcac tactctcggt tgtactttga aacctcttta tcataatttc
    14821 atttaatttt gcagtaagct tccagtgtac ttttttaaga ttatccccat atacatattt
    14881 ccttacatcc tgcagggcac ctatgtcttc aaacttgttc tgtaaagctg agaatgattc
    14941 tgacgtaaaa tctgttttta atctgaaccg ctccaatata ataactcttg gatatactgt
    15001 taagcttatt ggaaagggga ccttgtaaac aaatttaaag attcccaaaa aatcttcaat
    15061 ttcaattctt tccactccta tatcataatt gccgctgtat ttgcagaaaa gttcaaaagt
    15121 aaatattcta tcactgcggg gaagaagaga aagatttatt gtttcgaatt gtcgagaaaa
    15181 tatagtttgt gagccgaaaa aagtgacact catgtatgga tagaaaacat aatcttcatt
    15241 actgattgta aagatataat tgattttgtc accttttata ataaatttcc gatctatctc
    15301 ctggtgaaat ttaaatcgga agaaaattac caaagtatat aaaaatgata ccagtggtaa
    15361 tagaataatg cagaaaaaca gcatataagg gactatgcca ccgtaaaaat atgtaaatgt
    15421 tatcgataaa actaataagc ttgcataaag cactctgttt gccaacatta aaacctgcca
    15481 tcctctttat tctgttttac agggagctcc actccattac tcattgttcc tttatccact
    15541 acccactacc tactacccaa catccactat ccactatcta ccatccacta tccactatcc
    15601 actatcgcat taccttggaa cgtttacatt acctattata gatctaagta tgtcttcggg
    15661 tgaaatcttt tttagtttcg cttcctgctt taaaaaaatt ctgtgagata gtaccggtaa
    15721 agccatcttt tttacatcgt ccggcaagac atattctcta ccattataaa atgcccaggc
    15781 ctgtgatgcc ctgaaaagat tcaatgatcc tctagggctt gctcccagga ctacatcatg
    15841 atgtttgcgg gttgaatcaa caatttcaac aatataacgg tttaatgatt tatccacata
    15901 aatacttctt atcttttgct gaagagatat tatgtaattg ccatctgtaa caggctcaag
    15961 agcaagaaga gggttttcca ttttaaacct ttccagtata aggctttcat ctgccggtga
    16021 tggatatccc aaagatattt ttaaaacaaa tctgtccagt tgagcttcgg gaagagggta
    16081 ggtacccagg tattcaacag gattctgtgt tgcaaggacc ataaaaggcc ttgggacgtt
    16141 atatgttata ccatcaacag taacctggtt ttcttccata acctcaagaa ggcttgcctg
    16201 agttttagga gaagtcctgt taatctcatc agcaagtata atattactca ttaccgagcc
    16261 tggtttatat tcaaaatttc ctgttttttg attgtatatt gagaaacctg taatgtcaga
    16321 aggcagtatg tccggggtaa attgaattct tttaaatgag cagtcaatag atttcgctat
    16381 tgaagatacg aggctggttt tcccaactcc tgggacatct tctattagaa catgtccgct
    16441 gcaggccagt gcaataagag taagctcaat agcttcccgc ttccctaata ttaccttttc
    16501 aacattttct acaattagct tgaaagtttg aagggtatta tccatatttg cccccttgtt
    16561 aaatattaaa ttatgagact atagtattat agtatatcta tatagttaaa aagtgcaaca
    16621 ttcttaggtt ataacagtat ttaagtctga ggttataaca gtatttaagc actttttttg
    16681 gtataaccca ggtgcataaa tacacccata taacttcatt tatttatata ttaaatcatc
    16741 aagcaattat tgtaaagtat attttacaga tagaaaattc cataaaatat tgctatatta
    16801 tgtcccatat agtaaactta atatacaata tacagcaaac tcattttaca atatttagtt
    16861 tcaagcacac aaatgggaca ttttatattt cgcatgaaca gattttaata gaggatggcg
    16921 attttatgtt cagtagttta aaaaaccata gagtattgtc gttccttctg tatcaatgcc
    16981 ttgtatcaat gatacttgtt ttggcatgta cggcactttc aagtgccata tcatatgatg
    17041 tactccggaa aaaattatct gcctggagta tacgcaccaa caatgaattg atagaacact
    17101 ataatgatgt gttcaaagtt tcgattgtta ccggctctga gaaaatatat gaccaaacac
    17161 tattggcact atacagcaat tcaaatatga agtattttct gaataaccca attgaaaaaa
    17221 attttctggg cataaacagc ataaatacct atttcagaaa tattgtaaag gctaatcctc
    17281 tcatcaggga ggttggaata ttttatgtta cgaatcatct tatagttaca aatgatactg
    17341 tcaaatatga ttatttctat aactttcata atcatgaaat agcttttttt atatcaaaga
    17401 ttgaggaaat attagacaaa ggtgatgtga gtaattttaa tgcatacgcg acagatggag
    17461 ctattcaaat gataagacca cttgtgactg ctaataaggt tattgctctg atatttatag
    17521 aatacagtac tgatgcattg aatgaaaaat taaaagaaaa tgtgacggac atgtttggaa
    17581 acctgcttgt gctggataag caaggtaccg tgatatatga taacagcagc gggtattccg
    17641 gaaagcatta ctcacagctt cctatttaca gcgataagct gaataatgcc gacaggggct
    17701 actatttcaa aaatatagac aaggtccgca gcattatctc ccatatgtca gacaacaata
    17761 gctggcgtta tatttctata actcctgagc aattctacat ggaacctgta caatatattc
    17821 tgaaaaatct tattatatgt attcttgtat cacttctggc cagtataata ttcatagtaa
    17881 ccatgtcact gtggcaatcc agatctatga ggtcaattat tgagctttgc gataaaacag
    17941 gtgaaaacaa cagtattcct tctaaatttt cggacatata tgggtttata aggagcacac
    18001 tgactgaatt gatgagaaca gtcgaaactc agacacagga aataaaaaaa ctaatgccaa
    18061 tactacggaa aaactttgtt ctatggctta tgtctgaaaa agaggccgat gcaggcgagg
    18121 taaatgatac aatgctttta atgcgtgtag aattcccttt tgaaaacttt gcatttcttg
    18181 cagttaaagc agtttacgaa tatataaata ttgatgcgga aagtgatgag tttgatcccg
    18241 gctatgcatt agcagaagcc tctgtaatac ttaaagctgc attcaatact aatgattcta
    18301 tcggagtatt ctatcgaaac tcagattata ttacaggcct tatcaatttc agcttcgaca
    18361 gcaaaaccct tgatagacgc tgcaacactc tttttaagca atcgtttcac ggttttcgct
    18421 tctatattgc atcaggacct gttgcttcta atataagtga acttgcaggt atggcaaact
    18481 ccacaataga atccctgcgt tattcctatc tctttccgga aaacaagtat ttttcacatg
    18541 aatttgtgag tcgattcggg aaggttgagc ttaatatttc aaatgagctc tccatgtttt
    18601 ctgaagctat gaaacgaaaa gattcaaagt gtacgattga gcttttatat actatagttc
    18661 aaaagctgct ccatagcggc tgtacaccac aatatgcaca gcaggttatg ctggaggcaa
    18721 gctccattat tgaaaaattc agcaggcccg gaataaacga agagcttatt catatctttg
    18781 gagcttgtaa tgatatatta tcgctatatc aggcattaag agatattata agtttagaat
    18841 ttgactttat acctattgtt tcaaataaca cattggtact tataaatgat gcaaaaaagt
    18901 acatagacag aaatttgacc agctctcaat tatctttaca atcagtttcg gatgaactta
    18961 atattacaac acaatatcta agccagatat ttcacgaagt aagtggaata acattcacag
    19021 aatacattaa ctctaaaaaa atgaaactgg cttgccagct actgaacgaa actaatttaa
    19081 atataactga tatatcagaa aagctatgct attcatcacc ccagtacttc attagacgat
    19141 tcaaaaagtt ttatggggta accccgagtt cttatcgtca caatatgaaa ggaagattga
    19201 cagaagaagt cagcgaaatt tctttgctac attagatttt tgcaatgtaa tagaatcatt
    19261 gcaaaagaat cacaggtaaa tcttaaaaaa gtgcactatc ttaaatttgt accatttata
    19321 tccgctttta aaaatatttc cttctgaaag gagcattttt taagaactta agtaattacc
    19381 attgtaaaaa atccaatagg aaaatagaat ttgacttgag atataagtta tcttatagaa
    19441 atttgtatct acaaatctat cttttcaagg aggtataatt atgaagagga aacttataat
    19501 gacagtcatc tatgtactgc taattgtgtt tgtattaggc gcatgcacca cgggtgcaaa
    19561 taaaactgtc gcaaccgaca aaaccgggaa aaatgacaca accagtaaaa cagaggcaac
    19621 tgcttcagct caaaaagagc ctccagcaga actcagctgg ttctttcacc cccagggaac
    19681 aacctggcag gaatttgact tttctaataa ctggtttata gacattatcg aagaaatagc
    19741 taatgtcaag tttaccagtg taattactcc accccatgct gatgtttgga cgaaattcgg
    19801 tatgcatatc gcctctggtg aattaatgga tttaatgtat ggtggagcat ctatggccga
    19861 tcttaataaa tatggaaagg atggtgcgtt tttacaagtt gacaattata tagataactc
    19921 tcctattatc tcaaaagtct ataatagtgc acagcgtgaa tacataaaag cagaagacgg
    19981 caagacctat gttatctggg gttacccggg caatgcagat ttcggtacaa atgaaggctt
    20041 tggctacagg gcagacctgc ttgaaaaggt aggccttgat acacccacaa ctcttgatgg
    20101 atgggttgat gcaatgcgta aagttaaggt agaaattccc aatactatcc catactctac
    20161 tgttaattta gcccagtatt acgaattaat gttctcgcca ttcaatatta tgttcaatta
    20221 tggtacatgg atatataacg atgaaagcca tacttatacc catgcgtttg atacagaaaa
    20281 tatggtgaag gcacttacct ttggcaggat gctttttaaa gagggccttt tggataatga
    20341 attcatgact catacaaagt cagattacac caagaaaatt tatagcaaca acatgtttat
    20401 tgttgttaag aatcgtggtg gcatcagcgg ttttataacg aaatatcccg ctaatggtgt
    20461 tcaaggcgca aaactgttac cttcatggta cccagccgcc gatggcatag acatgaagga
    20521 tggtgtatat aaacactggg gtcagcttat aggcgcacca atggtaatca gttcaaagac
    20581 aaagtctgca gatgcctgta tccgtgttat agaaacatta ttaagtgata aagttagaaa
    20641 tttggtagtg tatgggcggg aaggctatga atatgaggtc gtaaatggtg tgaaaactcc
    20701 gattcagcct gcggaaaccg acaatggctg gagaattgga tatggcttaa tggttggagt
    20761 aaatacagca caacgtatgt cctataaaga agatgctgca attgatgcca gcccatcata
    20821 tgcttctgaa gaaaaagaag catataaaaa agcgttttca gaacgtgcgg caaaatttac
    20881 cgaattggtt tcaattgata caccgagaag caacttgtat gcagctcctt taagtgatga
    20941 actcagtatc aaaagaagtg attccttcga gctccagaag tcccttattg caaagacaat
    21001 catgggtgat ataagcattg atgaatttcg tgcagaagct aaaaaggttg ttgcaaacga
    21061 tgcggatatt gtggcggcaa tgaataaact tatgtcagaa gctgttaaga aatacggcct
    21121 gaaataataa aacaaactat tgaatttgac ggggcgcttc agtatgtgct gcgccccgtt
    21181 tatatgtaat atataaacaa tgggaggtat atatgaacaa ggaaatttca caaaccacac
    21241 taaaaagcaa aaaacctgat atattaatca gatttagaaa atctattcct gtttatatgt
    21301 taatactacc gagtactgca cttctactca tattccatta tttacccata ttcggcatct
    21361 caatagcctt tcaagattat agtgtataca aaggagtttt cggtagcaca tgggttggta
    21421 taaaatattt cgaacgtttt ttaactgatg ctaacttttg gcgtgttatg cgaaatacaa
    21481 tcattataaa tgtatatgat ttgatttttg gctttccggt accaattata tttgcattgt
    21541 ttttaaatga attgcgttca aacaaactta aaagggttgc acagactatc agctatctgc
    21601 cacattttat ctcgtgggtt gttgtagctg gtatggttgt tacaatacta tcaccttcga
    21661 cgggattgat aaacataatg ctcaaaaaca tattcaatac agaaccaata cactttctcg
    21721 caaaaccaca gtatttcaga tctattttag ttacagccga tatctggaag cactttggta
    21781 tgagcgctgt atattacata gcagcacttt catctattga catggagctc tatgcagcag
    21841 catcaatcga tggtgccgga cgcctgcgtc agacatggca tatcacactt ccgggtttgc
    21901 gcttgataat tatagttttg ttagttctaa agatgggcaa aatgataaac attggtttcg
    21961 aaaaagtatt tttgctttac aatcccatac tatatgaagt aggtgatgta ataagcacct
    22021 atacttaccg tctcggtatt gagaagaacc agtattctct aacctcggcc ataggtctta
    22081 ctcaatcggt aataaatttt attttagttt tttcggcaaa caagctatca cgtaaaattg
    22141 ccggatggtc gctttggtaa aagaaagggg gcaataatat gaaacaaact cgaggcgaaa
    22201 aaatattcca gtgctttgat atttttataa tattactgat aattactgca gttatattcc
    22261 cacttctcaa tatttttgca atctccgtga gcgatgcatc ctcaatagta aacggcaagg
    22321 ttgcactttg gcctaagggt tttaataccg cagcatattc gaaaatttta tcaaacaatc
    22381 attttattcg tgcgatgcta aatactattt taataacagt taccagtacc ttcctatcag
    22441 tgttgctctc actgatgacc gcctatggat ttacaaaaga gtttcttggc aaaaaattta
    22501 taacatatct atatgtgctg accatgtatt tctcaggcgg tcttattcca acttatgttg
    22561 tatatacgaa atacctgcat ttgcgtaaca attttcttgt tctgatactt ccgggtcttg
    22621 tcagcatgtt ttatataatt attgtacgca gtcagattga aagcctgcct ttgagtgttt
    22681 tagagtccgc ttacatagat ggtgcaaccg aatttcaaac ggcattcaaa attgtactac
    22741 ccatgatttc acccactctt gctgcaattg caatgtttac tgccttagga aactggaata
    22801 tctggtttca ggttatgatt tacacagatt atcctaaatt ctggacatta caatactatt
    22861 tacgtacaat tgtatttggc caattcctgg cggcgaatga gtttataaca gaagcagcag
    22921 gtgaatttat tcccgaggag aattaccgaa tggctgcaat aattattgtt gctgcaccga
    22981 ttgtatgtat ttatccattt gtgcagaaat attttgttaa aggtatcatc tcaggctctg
    23041 taaaaggata gggtatttag ttgtgaaaga gttcaaagag taaaaagaag ggcattgaaa
    23101 ggagataata aatatgcaat gggaacattt ggtgccatcg gattttagga aggctgtcaa
    23161 tgaaacaagg ctatgtatat tacccactgg ttctcttgaa cggcatggtg agcatttgcc
    23221 ttttggctgt gacatgattg ttgcacatac tatagcatgc aaggctgcag aaattgaacc
    23281 tgctgtggta tttccacctt attatataat gcaggtacat gaggcagcct gcttcaccgg
    23341 tacggtcaat tatcctcagg cttttgcact tgaggcattg agactacttt tggagtccat
    23401 agcagctaat ggttttagaa aaatacttgt tgtcaatgga cacggaggca acaagcacat
    23461 gcttgattat tttgcaatga gccgggttga tgaacttaca gaatatattt tttatcaaac
    23521 aggtatctat gggaaactaa cccaagagga agataatatt ataaagaatg tgtttggcac
    23581 caattctggt ggacatgccg gtgaactgga gacatcactt ttcatggcat gctgtccggg
    23641 caaagtaaaa cttgagtatg cccagcaaaa accagttatg ccgctaaagc gctttaagca
    23701 tttgtgggat aatggtgtta acaacgcttt atggtggtat gcagactatc ctgaaaatgt
    23761 agcaggtgat gcccgtcttg cgtctgaaga aaaaggcaga gtggtattgg atgtttacgc
    23821 caaatcactg gcccgtgcca taaagacggt caaggaagac actgaagcag ttcgtttaca
    23881 gacagagttt ctaaaatatg tgaagaataa aggaaactat ggaggctaag ccatatggaa
    23941 cttttgaata ataaacttaa ctggtataaa ggaaatttgc attgccatac aaatatttca
    24001 gatgggttaa tttcaccatc ggaatgtata gatgcatata agaaagcagg atatgatttc
    24061 ctttctatca ctgatcatgc aaaatattat aaagggtatg aaacggaaaa ctttgtttta
    24121 tttagcggaa cagaatttca tcatgtgaat tgggtaacaa gaaagaattt tcatatttta
    24181 ggtataggt
//
LOCUS       DAOSDD010000036        24180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_935_length_24180_cov_5.125762, whole genome shotgun sequence.
ACCESSION   DAOSDD010000036 DAOSDD010000000
VERSION     DAOSDD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_24180_cov_5.125762"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1387)
                     /locus_tag="PK733_05105"
     CDS             complement(140..1387)
                     /locus_tag="PK733_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ09954.1"
                     /translation="MKIGYSSIDITPEPGVELSGYGWFLERKAEGVLDNLYARAVAFE
                     GDNKRMLLINCDLVAIDKPISDAVKQQISNEISISKTNIMIVCTHTHTGPATGRLIGC
                     GEPDEKYRSVLIDLLIGAGKSAFNNLMEIASVKSVTKEIEPIGFNRVSKDGPVDNVVR
                     GMVFYFDEGRPLAILSYGCHPVTLGPLRHVSADYPGRVIKALNEEGFDGVFLTGLCGD
                     IDPVQNLIKWGSGTPEIIDEYGRRIVNVLLDSVSKTEEMKESSLDAYEIDLSLKLQKY
                     KHEDIDRQVEIYERKKEENPGAYKVAKIWAAEMKTQLERSKEPYLEFLTVQIFRIGNV
                     LLIGFPGEVFTEIGSIIRESLPDFNVLALGNANSTMRYVPTRDDIEKQGYAGLLSCLL
                     YLRLPLEPGEGERMAEIVAGQIR"
     gene            complement(1515..3434)
                     /locus_tag="PK733_05110"
     CDS             complement(1515..3434)
                     /locus_tag="PK733_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09955.1"
                     /translation="MENKKVKCEYLCDPCRNFCFLGAISIKDHKDDREKAVLSSFVSG
                     GTGGLVFIDPETQLGEWIELPGDEGAWALLSLDDKKLLVGTCGLKGYLHCLDLKSRTW
                     AEPLRDENETYIWNLVQGSDGMVYGGTYPGCVLLQYDPQKHILKNIGKMSHYSGNMYS
                     RTVWRVGNYILINCGMDKPHIVAWSVETGSVKPFGKENAQIKEISTNYICTETNGELD
                     FYNTETFELIKNNSDILKVDKEQNRSNQSGANQNEANQGRNIQSGNNQNINNKTYQRA
                     LSDKGIGRILELKDNRIFGIRGQEYFISNKNDDALTLENGESLTLKKIPVDAPATGIL
                     TITTDNNGCIWGSSNFGQTIFCYNTSGGGHLSPRYNTKSGEYFNTPAVCNNGGEVYGM
                     KCINNKIFMTCYAGGDHVVYDPEKPWDQFNNVNPKTLKSVAPELIRPGAKSVIGPDGA
                     LWTGWTAKYGVYGGGVSRVDPEIYNVTVWKDLIPGQSIRSIASGKKNLYFTTSGWGNG
                     LPEKVEPLFLAVMDIEGKLIWKREFPKEQKLGLIEIVGDLGIVNIDSKIEIFNCHTME
                     FSNSIKMKAPCSCIIKCSENIAALFCGKELYFLKPAEGRLEYVLDLPGAVSTATIDSK
                     GNLYFAYKSGLYKVA"
     gene            complement(3535..4377)
                     /locus_tag="PK733_05115"
     CDS             complement(3535..4377)
                     /locus_tag="PK733_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09956.1"
                     /translation="MAIIKVPTNYYQHFDADFSKDIPGEGYGGWKKALLPLNTEKTAL
                     AVMHAWDCGTREQYPGWHRAVEYIPRSYEILRTEFPKILSASRQANMNIVHIASGDEY
                     CKDYPGYKIVKELEKKHIREEYKQNFINSEDEVLKEIYRFRYANVFPGEHNVADIKAG
                     QSKMTFAKEAEPLNDEAIAKDSTQLLAYCIENKINHLIYIGFAIDGCLLLSPGGMVDM
                     SRKGILCSTIRQAVTAIENKETAREEICKQVALWRVALLYGFVYDSDDFVKGLLSLYE
                     EKLD"
     gene            complement(4415..5632)
                     /locus_tag="PK733_05120"
     CDS             complement(4415..5632)
                     /locus_tag="PK733_05120"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ09957.1"
                     /translation="MAGLAINGGNKMRHIPFTQWPQWRSKEVELLEKVLKSGVWGTLG
                     NEALGFADRFAKYQDAEYGISVHNGTISLELIIRGLNIGYGDEVIVPAYSFISTASSV
                     AIAGATPVFTDIDPNTYNIDPASIEEKITPKTKAIVVVHIGGRPCDMDKVMEIAYKHG
                     LNVIEDAAHAHGSEWKGRKVGAIGDAGSFSFQNSKNMSCGEGGAIITNNYEHFKEFWH
                     YHHSGRAYEMSSEFGGLVLMGTNARMAEWEAAVLNAQLDKLDEQIEIRMKNASYLNSK
                     LKEFDFIQLLSEDERITRNSHHLYIIKYDSKKLGNLSRNKLISAMCAEGIPCSGGYIP
                     LYKMDAFNSANFRKSTGSETDYSKLYLPKTEEASNDGAIWFSGNMLLGAKEDMDDIIR
                     AMEKISGNANELV"
     gene            complement(5733..6959)
                     /locus_tag="PK733_05125"
     CDS             complement(5733..6959)
                     /locus_tag="PK733_05125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ09958.1"
                     /translation="MSTLAIKGGEPLRTKSFTSWPKSGEEELERLKRVLNSGQWGTLG
                     AETEKFAKRYAAYCNAKYAVATPNGTVSLEVILRALNIGRGDEVILPPYTFVATASAI
                     TFAGATPVFADIDENTYNLDPANIEQHISQRTKAIIAVHLGGRPCDMDKIMDVARKHK
                     LYVIEDAAHAHGSEWKGRKVGSIGDVGSFSFQASKNLCCGEGGAITTNNENLYKSIWS
                     VHNCGRDYDSNIWYQHPVLGTNARMAEWQAVILDVQLNKLDGEIEKRMTNAAYLTKRL
                     KETGFIDALYEDPNITKNSHHLYVFKYKKERCWGIHRDLFVKALHAEGIGLCATGYSL
                     PIYRMVFLDSDAFRKATGCTVSYKDISLPVNDRAAYEEGAWLYHTALLGGKEDIDDIV
                     DAMLKIYEHADELSNE"
     gene            complement(7060..8289)
                     /locus_tag="PK733_05130"
     CDS             complement(7060..8289)
                     /locus_tag="PK733_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ09959.1"
                     /translation="MKQIGLNTIKAQNIKLLFDIIVNNDKITRNDAARLSNLSLMTVC
                     NIVDHLDRFGIIHHVDKEQANTVGRKAELLSINKDIKRFIIIDLTSLEFSFTILNLDL
                     TIHNTFKEWRYNFERNYTDNLEDFLLITDKYINTHFPVDETISIGVVVPGPYDVENDI
                     VINKRIPELMQLNIKSIISETLIRGKYIEDDTLEDNILETNILEDYILDIFIDEDVKF
                     AAQANITTIPDYTGKIIYYAYIGEGVGGAISVNGSILRGLFSFAGDIGQVLADENTCF
                     EELISLKAFAREVAGNDNITGSDEDIMKMLEDYQADTDNFHDFRRRLMDFCKRISSAL
                     YNVIWFIDPHAIIIECRYAGLDHDFFIEGIKTMLTQMLLPIRQDIPEIFLSNHIVKNA
                     HVGAGILLRNRWLKSIS"
     gene            complement(8339..9073)
                     /locus_tag="PK733_05135"
     CDS             complement(8339..9073)
                     /locus_tag="PK733_05135"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ09960.1"
                     /translation="MSKAMDYISNIERLIKEVKETQIQALEEAAKTISQCLISGGMIY
                     TFGTGHSHMLAEEIFYRAGGLVRVNPILDEGLMLHAGAVKSTSLERLHGYAKALLDNY
                     PVQKDDVIIIASNSGRNTVSIEMAIEAKKRGMKVWALTNLKHSKSVASRHESGKLLYE
                     LADIIIDNCGSIGDASMEFDRIGNVGPTSTVMGAMLLHAIICEAIEIMLGKNVVPEVF
                     SSSNVDGGDRINKSLIDKYSKKIKSL"
     gene            complement(9114..10076)
                     /locus_tag="PK733_05140"
     CDS             complement(9114..10076)
                     /locus_tag="PK733_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ09961.1"
                     /translation="MRDLADRIFVGVDGGGTSTNIIAVTDKGQVLGKTVGEGTNFYAT
                     NMSAARSNLKQAIDRLIDECGISDYEFISIGMSSLDDEPAEDVIIAFAGKVFQPEKIE
                     MQSDVYMALMGMTLGKSGIMVVSGTGSMAIAVDQTEKLHILGGWGYLLQDEGSAYHIA
                     IEGIKAGIRSFESTGEKTILEKRVTNFFNVQSYRQLIDVLYNPPMQNSKLAKFAKEVE
                     KCAKEGDSIAASIVDRAVAILVQYVCRLMDILRIDDCIVGMYGGVFQNSPYISSSFIS
                     QVHTLYPKAEISFPELAPEIGAVLYGMKKRGYPVTQEFLDNLKV"
     gene            complement(10305..12365)
                     /gene="recG"
                     /locus_tag="PK733_05145"
     CDS             complement(10305..12365)
                     /gene="recG"
                     /locus_tag="PK733_05145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOJ09962.1"
                     /translation="MNNNSEDILKRPISCLKGIGAAREKLLNKIGVFTVKDLIQYYPR
                     DYEDRSRLVKINNLQNEENSAFIGTITSKLRETSHGARMTIQKIEISDDTGIAYAVWF
                     NQPYLKNVFRPGESYFFYGKIKRAGSFIEVQTPVYEKSGDHEKTKDSFCNIVPVYSTT
                     VKLSQAVIRSAAKEGLKQVDGIIPEILPQNIREKYLLSEINYAIQNIHFPHDNEALNN
                     SRYRLVFEELFSLQLSLLAVKKSFNEGKKGISFKKGENVHDLVKSLPFQLTNAQLKVF
                     KDIETNMESSGVMNRLIQGDVGSGKTIVAVLALLKAVENGYQGVLMAPTGILAEQHYR
                     LVKGLLQKYNVNTGLLTGNITGKEKSKVLEGIKNGDIDIVIGTHALIQDGVECYNLGL
                     VITDEQHRFGVRQRACLSKKGENPDILVMTATPIPRTLALILYGDLDISVIDELPPGR
                     TPVKTYIVNKSMRERINNFIRKNAEQGRQVYIICPLVEDSDDIDAMSTITLAEDIAKK
                     DFKDLKVGLLHGKLKPDLKEQVMKDFIEGKIDILVSTTVVEVGVDVPNATIMVVENAE
                     RFGLAQLHQLRGRVGRGKDQSYCILYNESNSPIAKERMQVMEKTCDGFIISEKDLELR
                     GPGEFFGTKQHGIPELKIANLYKDIELLKIAQQAASDMLKATSDILIEREPGWLHVNT
                     HSTL"
     gene            12563..12751
                     /gene="rpmB"
                     /locus_tag="PK733_05150"
     CDS             12563..12751
                     /gene="rpmB"
                     /locus_tag="PK733_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOJ09963.1"
                     /translation="MAKCFICEKETTFGNQVSHSIRRTSRSWKPNIKRVKALVDGSAK
                     TVYVCTRCLRSNKVTRSV"
     gene            13392..13886
                     /locus_tag="PK733_05155"
                     /pseudo
     CDS             13392..13886
                     /locus_tag="PK733_05155"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
     gene            complement(13914..14582)
                     /gene="nth"
                     /locus_tag="PK733_05160"
     CDS             complement(13914..14582)
                     /gene="nth"
                     /locus_tag="PK733_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ09964.1"
                     /translation="MGNKVEEKNRQKNIKRVNEIINVFDKLYPEASCSLHYKDPLQLL
                     VATQLAAQCTDARVNIVTKDLFKKYKTVEDFANADQSELENDIKSTGFYRNKARNITN
                     CCKMMIEHFNGKVPDNLEDMLKLPGVGRKTANLVLGDIFGIPGVVIDTHAARLSKRIG
                     LTKNDHPEKIEYDLMEIVPKQEWTKFCHQLVYHGRAICTARKPQCTRCPIREHCDYGQ
                     NLSI"
     gene            complement(14627..16069)
                     /gene="glgA"
                     /locus_tag="PK733_05165"
     CDS             complement(14627..16069)
                     /gene="glgA"
                     /locus_tag="PK733_05165"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOJ09965.1"
                     /translation="MHHKGKLKILYVSAEVSPFAKTGGLADVAGSLPKALAAGGNDVR
                     VVMPGYKAIKSPMKYVVDFPVKMGDNVKTCIIRETEASLPFYFVDSYDYFDRDGIYCH
                     FDDAERFAFFCNSVLEMLPKINFQPDIIHCNDWHTGPICMLIKERYNNISFYSGISSV
                     YTIHNLEYQGNFPREVTSLFNVGGEVFIPDKVEYYGMFNFMKAGIVYADIINTVSSSY
                     AKEIQTPQYGEGLDGLLRKRGKDLYGIVNGISYEDFNPSTDKRIYKNYDIKSLEAKKE
                     NKYALQEEMGLPAGDMPVIGLVSRLSGQKGLDLIIERIDDIMKHDLQFILLGTGDPYY
                     ESIFTGIKNRYPEKMSAFIGFNAVLAQKIYAGCDMFLMPSRFEPCGLGQIISLRYGTI
                     PIVRSTGGLAETIIDYDEDRENGNGFSFTEFSPDELYITIERALSLYNRKPEEWKMLV
                     ERALTRDFSWNRSAEKYMELYYIAIEKKLV"
     gene            complement(16107..17549)
                     /locus_tag="PK733_05170"
     CDS             complement(16107..17549)
                     /locus_tag="PK733_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ09966.1"
                     /translation="MFDDDNNYRLNKDDIKKINDDGSEKFSENSDHTSGSLYNSNNSS
                     SYSNINYSNSTYSGRSNSQGSPFYYSESYNKKKSKKGYRLNMLQLVAIVLISSILGGG
                     VVFSAFQFIAPAIQPEFSSYFDRMTGKNVASNSPANGQDAGKSYKIEIEKTNSPVTWI
                     AKKVSPSIVGIRVTAKVQNFFFGEQQDKGEGSGIIVRDNGYILTNFHVIEKAYDSRTN
                     KVSGSATVEVFLPNQEDKPYKAEIVGVDWRTDLAVLKINGTGLPAAELGDSDALEVGE
                     LAVTIGNPGGLEYMGSVTAGVISGLNRRVQTENGYEFKLIQTDAAINPGNSGGALVNS
                     EGKVIGVNSIKVVAQGFEGIGFAIPINTAKDIFENLIEHKYVPGRPLLGLRADPDFNS
                     EAARYYKVPEGVLVKEVIPFSGAYKAGIKVGDIVVKCEGTPVKSLDELNTIKDKYKPG
                     DIVKLEIYRDNKTLTIEVELGEDKPLFDEQ"
     gene            complement(17720..19213)
                     /locus_tag="PK733_05175"
     CDS             complement(17720..19213)
                     /locus_tag="PK733_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ09967.1"
                     /translation="MFKSIFSRLVAMFIAIIIFSFSISGITLYYSFGKYVSEEKIRTL
                     DASGEEISKYLKFYLENMQNPVAEVMLDYVLNSYRQNTGSYIWITNDKGVIVFASPYL
                     KLTDSKILNHLKYEQGTYILPDERQYKKVLSGTNPVIEKGDFYGLFKETGWSWLVVQK
                     PFKYVDTAGAEHVVAAVYLSTPITEINKARSTVYRFFLISVVVSVLISTFLVYFFSRR
                     ITNPLKQISNAAKIISGGEFNKRLNINSRDEIGELAGSFNQMAVALENLEEMRRGFIA
                     NVSHELRTPMTSIKGFIEGILDGTIPMEKQKDYLNIVRDEVMRLNRLVNDLLDLAKME
                     SGEIHLMFSNFNINELIRRCIIKLEHLITEKELEVTVNFDEETFVSADLDAIERVLIN
                     LIHNAVKFTPNKGIIGINTIIDADRLIISVADTGIGIDKEELNMIWDRFYKTDKSRSR
                     DKLGTGLGLAIVKNIINEHGQEIWVESEPGKGTTFHFTLKRADESLI"
     gene            complement(19213..19899)
                     /locus_tag="PK733_05180"
     CDS             complement(19213..19899)
                     /locus_tag="PK733_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ09968.1"
                     /translation="MINKTKVLVIDDDVNICELIRLYMEKEGYEVLAVYNGKKAIEEF
                     SRFAPNIVILDIMLPGADGWQVCREIRKISNIPIIMLTAKGETFDKVLGLELGADDYM
                     VKPFEPKELVARVKAVLRRYERKELDTEEVVYPNLVINKTNYTVKIVGNEMELPPKEL
                     ELLFFLASNPNKVFTREQLLEHVWGFDFYGDSRTVDVHVKRLREKLDLPNQVWQLKTV
                     WGVGYKFEVK"
     gene            complement(19912..20616)
                     /locus_tag="PK733_05185"
     CDS             complement(19912..20616)
                     /locus_tag="PK733_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ09969.1"
                     /translation="MNKYDIHVHTAETSPCGKVDAIDVVKLYKEAGYAGIVITDHYYK
                     GFFDRSTGGNWQMKIEDYLKGYKKALKEGASIGLNVLLGMEIRFNDNPNDYLVYGIDE
                     EFLRDNPELYNLTLGEFRNFISNASYMSNKNILIFQAHPYRQGQTIEEPELLDGIEAY
                     NGNPRHNSQNDLAYNFAIENQLRMISGSDFHQVVDLARGGIIIKDSIANIKELVKILK
                     ENDIIDLIRAENKESI"
     misc_binding    20706..21019
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21124..22140
                     /gene="aroF"
                     /locus_tag="PK733_05190"
     CDS             21124..22140
                     /gene="aroF"
                     /locus_tag="PK733_05190"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ09970.1"
                     /translation="MIVVMSQNATKEQIINVERKLLELGFSTHPIYGEIKTVIGAIGD
                     KLLLNTQAITTMPGVENLVPIMKPFKLASRELKQESTIVEVGDVKIGGPEIIVMAGPC
                     AIENEESFMDTAFKVKETGAKILRGGAFKPRTSPYSFQGLEEEGLKIMAKAREATGLK
                     IVTEVIDTRDVETVSFYTDIIQIGARNMQNFRLLQEAGSTGKPILLKRGLAATIEEWL
                     MAAEYILSSGDSNVILCERGIRTYETATRNTIDMSAIPVVKELSHLPIIVDPSHATGT
                     WKYVAPLSKGAIATGADGLIIEVHSDPTCALCDGPQSLKPSKFSQLMEELEPVALAVG
                     RKLR"
     gene            complement(22215..22817)
                     /locus_tag="PK733_05195"
     CDS             complement(22215..22817)
                     /locus_tag="PK733_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ09971.1"
                     /translation="MNNKKRCININVINVKEYKYILYIIVVFLLIVLIINNSTTIIKR
                     IYPLKYQELVFSYSEFNGIDPYLVFAVIKAESSFNPYAKSKKDAMGLMQITENTAKWG
                     AESMDIKSFKIEDLYDPETNIRIGCWYIKQLMVEFKNDIDLVLAAYNGGSGNVNEWLR
                     NNTYSKTGKKLDVIPFRETDTFIKRVRKYQQIYIRLYSER"
     gene            complement(22907..23512)
                     /gene="coaE"
                     /locus_tag="PK733_05200"
     CDS             complement(22907..23512)
                     /gene="coaE"
                     /locus_tag="PK733_05200"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520919.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOJ09972.1"
                     /translation="MKVIGVTGGIGSGKSTVTRILSDLGAEIIDADKIAREITAKGEP
                     ALQELVDYFGPGILNDNGELRRTKLAEIVFVDKEKLDILNSITHKYVVKRLISHLEKL
                     RYENKTSFTVLDVPIPVKHGFLDIVNEVWVVTADKETRIKRVMERNKISYEEAVNRIQ
                     SQKSDEEYLRLANEVIINNGTLEELEGTIARLYVQKKLGQV"
     gene            complement(23560..>24180)
                     /locus_tag="PK733_05205"
     CDS             complement(23560..>24180)
                     /locus_tag="PK733_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOJ09973.1"
                     /translation="LISAFINNEDIHASTASKVFDVPLDQVTPLMRSRSKAINFGIIY
                     GIGDFSLAKDLGITRKQARKYIDDYLDKYPGVRKYMKDVIDEGKEKGYVATLFNRRRY
                     LPELLSKNFNIRSFGQRVALNAPIQGTAADIIKIAMVKVYNQLKENNMKSRLILQVHD
                     ELIIETHVDEKNEVIKILKECMETAVSLNVPLKADIKTGENWYETK"
BASE COUNT         6895 a         5098 c         3753 g         8434 t
ORIGIN      
        1 agcttcactt atgctcctta ccaatatttt cttcacttaa attatatgca cccaatttgc
       61 agggtgcatt tactgaattt ggagaaatat ttaccagggg ctgtcgttac agtgcttgca
      121 cccaactatg tgtgttccct taccttatct gccctgcaac tatttccgcc atccgttcac
      181 cttctccggg ttccagaggc agcctaagat aaagtaggca ggatagtaat cccgcataac
      241 cttgtttttc aatatcatct cttgtaggta catatctcat agtggaattg gcattcccca
      301 atgctaaaac attgaaatct ggtaaggact ccctaataat agagccaatc tctgtgaata
      361 cttcacctgg gaaccctatc aaaagcacat tacctattcg aaaaatctga acagttaaaa
      421 attccaggta tggctccttg cttctctcta attgggtttt catttcggca gcccatattt
      481 ttgcaacctt atatgctcct ggattttctt ctttcttgcg ttcatatatt tcaacctgtc
      541 tgtctatatc ttcatgctta tacttctgca gcttaaggct caagtctatt tcatatgcat
      601 ccagacttga ttccttcatt tcttcagttt tagaaacgct gtctagaagt acgtttacta
      661 tccgcctgcc atattcatca attatctcgg gagtgcctga accccatttt ataagatttt
      721 gaacaggatc aatatctccg caaaggcctg ttaagaatac tccgtcaaag ccctcctcat
      781 ttaaggcttt tattactctg ccggggtaat ccgcagatac atgccttaat ggtccaagtg
      841 tgacaggatg acagccataa cttagaatag caagaggcct tccttcatcg aaatagaata
      901 ccatacccct tacaacatta tcgacaggac catctttgga taccctgtta aagcctatag
      961 gctctatttc ttttgttacg cttttaacac ttgcaatttc cattaaatta ttaaaggctg
     1021 atttacctgc tcctataaga agatcaatca aaacactgcg atatttctcg tcaggttcgc
     1081 cgcatccgat caatcttcct gtagcaggtc ctgtatgagt atgtgtgcaa acaatcataa
     1141 tgtttgtttt gctaatacta atttcattag aaatctgctg ttttacagca tcagaaattg
     1201 gcttgtcaat tgcaaccaaa tcacagttta ttaacagcat tcttttatta tcgccttcaa
     1261 atgctactgc ccttgcataa agattatcca gcactccttc tgcctttctc tccagaaacc
     1321 atccataccc gcttagttca acacctggtt caggtgtgat gtctatggaa gaatacccga
     1381 tcttcataat tttttcactc ctataaacat atttgtgaca agaaatatgt tttaatcttc
     1441 ctgccgcata aatgaaatct tatgttacct ttaacttgct accctttaaa taaatttatt
     1501 tatattactt tatattatgc caccttatac aacccagatt tatatgcaaa gtaaagattc
     1561 cctttagaat caatagttgc cgtactaacc gcacctggca agtcaagtac atattccagc
     1621 ctgccctctg ccggttttag gaaataaagc tctttaccgc agaatagggc tgctatattt
     1681 tcagaacatt ttattatgca ggaacaaggt gccttcattt ttattgagtt tgagaactcc
     1741 atagtgtgac aattaaatat ttcaatttta ctatcgatat ttactatacc aagatcccca
     1801 actatttcaa tcagacctaa cttctgctct ttaggaaatt ctcttttcca aatcagtttc
     1861 ccttcaatat ccattactgc aagaaataaa ggttcaactt tttcaggtag accgttgccc
     1921 catccgctgg tagtaaagta gaggtttttt ttccctgaag ctattgatct tatactttgt
     1981 cccggtataa gatctttcca tactgttaca ttgtaaattt cagggtcaac ccgggaaact
     2041 cctcccccat ataccccata ttttgctgtc caacctgtcc ataaagcccc atcagggcca
     2101 attacgcttt tagctcccgg tcttattaat tcaggagcaa ctgattttaa agtttttgga
     2161 tttacattat tgaactgatc ccatggcttt tccgggtcat aaactacatg gtctccccct
     2221 gcataacagg tcatgaagat tttattgtta atacatttca taccgtatac ttctccgcca
     2281 ttattacata cagcaggagt attgaaatac tcaccacttt ttgtattata acgaggcgat
     2341 agatgtcccc cacctgatgt attataacaa aatatggttt gaccaaaatt actagatccc
     2401 catatacatc cattgttatc agttgttatt gtcaaaatac ctgtagcagg ggcatcgaca
     2461 ggtatttttt tcagggttaa agattcacca ttttcaaggg ttaaagcatc gtcattttta
     2521 tttgaaataa aatattcctg gcctctaatt ccaaatattc tattatcctt taattccaat
     2581 attcttccta ttcccttatc agatagtgcc ctttgatacg ttttattatt tatattttga
     2641 ttgtttccgc tttgaatatt tcgaccttga tttgcttcat tttgatttgc tccgctttga
     2701 tttgatctat tttgttcttt atcaactttt aatatatctg aattgttttt gatgagttcg
     2761 aaagtctcag tattataaaa gtccagctca ccattagttt ccgtgcatat gtaattagta
     2821 cttatttctt ttatttgtgc attttccttt ccaaaaggct tcacagagcc tgtttcaaca
     2881 ctccaagcta caatatgtgg tttatccata ccgcaattaa ttaatatgta gtttccaacc
     2941 cgccacacgg ttctactata catattgcct gaataatgtg acattttccc tatgttttta
     3001 agtatatgct tttgcgggtc atattgtagc aaaacacatc caggataggt tccgccatat
     3061 accataccgt ctgaaccctg taccagattc cagatatatg tttcattttc atctcgtagt
     3121 ggttccgccc atgtacggct tttcaggtcc agacagtgca gatagccttt taagccgcaa
     3181 gtgcctacca aaagcttttt atcatcaagt gaaagcagtg cccaggcacc ctcatctccc
     3241 ggtaattcaa tccactcccc taattgagtt tccggatcaa taaaaaccag cccgccagtt
     3301 ccacctgaaa caaaagacga taatactgct ttttcacgat catccttatg atccttaata
     3361 gatatagctc ctaaaaagca gaaatttcta caggggtcac ataaatattc gcattttacc
     3421 tttttatttt ccatttttta atagctccta ttcttgcata taaaattaat ttgaatcatt
     3481 ttccggggtg catttaattt cttctaacaa attagatgca ctctgttctc cggtttaatc
     3541 cagtttttct tcatacaagc ttagaagtcc ctttacaaaa tcatctgaat cataaacaaa
     3601 gccatataaa agggctactc tccataatgc tacctgtttg cagatctctt ctcttgcagt
     3661 ttctttattt tctatagcag taacagcttg cctaattgta gaacataata tgccttttct
     3721 gctcatatct accattcctc caggtgataa aagcaagcat ccatcaattg caaatccaat
     3781 atatattaaa tggtttattt tgttctcaat acaatatgct aaaagctggg ttgaatcttt
     3841 tgctatggcc tcatcattaa gaggctctgc ttcttttgca aacgtcattt ttgattgtcc
     3901 tgctttaata tcagcaacat tatgttctcc cgggaaaaca ttggcatacc tgaatctata
     3961 tatttccttc aggacctcat cttcggaatt tataaaattc tgtttatact cttcccttat
     4021 atgcttcttt tcaagctctt ttacaatttt atatcccgga tagtctttgc aatattcatc
     4081 tcctgaggca atatgtacaa tattcatgtt tgcttgtcgg gaagcactaa gtattttggg
     4141 aaactcagtc cttaatattt catatgacct gggtatatac tcaactgccc tgtgccagcc
     4201 aggatattgc tcccgcgtac cacaatccca ggcatgcata acagcaaggg cggttttttc
     4261 agtgttaaga ggcaatagtg cctttttcca ccctccgtat ccttcccctg gtatgtcctt
     4321 gctaaagtca gcatcgaaat gttggtaata atttgttggt acttttatta ttgccatact
     4381 atttgcacca cctgttcatt tttagttatt aaagctatac taattcattt gcattgccgg
     4441 atatcttctc cattgcccgg ataatatcat ccatatcttc ttttgcacca agcagcatat
     4501 tcccagaaaa ccaaattgcc ccatcattag aagcctcttc agttttggga agataaagct
     4561 ttgagtaatc tgtttcagaa cctgtagatt ttctaaagtt tgcggaatta aaagcatcca
     4621 ttttatagag tggaatataa ccaccactgc atggaattcc ctctgcacac atggcagata
     4681 ttaatttatt acggcttaga ttaccaagtt ttttgctatc atattttata atatacaaat
     4741 gatgggaatt cctagttatt ctctcatctt cactcaatag ctgtataaaa tcaaactctt
     4801 ttaattttga attgagatat gaagcatttt tcatacgtat ttcaatctgt tcatcaagct
     4861 tgtcaagttg cgcattaagc acggcagctt cccattcggc cattctggca ttggtaccca
     4921 ttagtaccaa cccaccgaat tcactggaca tttcatatgc tcttccagag tggtgataat
     4981 gccagaattc tttaaaatgt tcgtaattat tagttataat agctccaccc tcaccacagg
     5041 acatgttttt actattttga aaactaaatg atcctgcatc acctatagct ccaacttttc
     5101 tgcctttcca ttccgagcca tgagcatggg ctgcatcttc gataacattt aacccgtgct
     5161 tataggctat ttccatgact ttgtccatat cgcacggcct gccccctata tgcacaacta
     5221 ctatagcttt tgtttttggg gttattttct cttcaatgct ggcaggatca atattgtaag
     5281 tatttgggtc aatatcagta aatacaggcg tggcaccggc tatagcaaca gatgatgcag
     5341 tagatataaa cgaataagca ggaactatta cctcatcacc atatcctatg ttaagaccac
     5401 ggataattaa ttccagacta atggtaccat tatgtactga tattccatat tctgcatcct
     5461 gatatttagc aaacctatca gcaaaaccaa gagcttcgtt acctagagtt ccccatacac
     5521 ccgattttaa aaccttctcc agtaattcga cttctttgct tctccattgc ggccattgag
     5581 tgaatgggat gtgtctcatt ttattgccgc catttatagc taaaccagcc attgtcatct
     5641 ctcccttcta aatctatagc attattcaaa tcaccgttat acttaatcac cattattcct
     5701 ggttgtctcc attccagcat agcagtacat tcttattcgt ttgaaagctc atcagcatgc
     5761 tcataaatct tcagcattgc atccacaatg tcatcaatat cctcttttcc gcccagtaaa
     5821 gcagtatgat aaagccatgc cccttcttca taggcagccc gatcattgac agggagactg
     5881 atatccttgt atgatacagt gcacccggtt gccttcctaa acgcatcact atctaaaaat
     5941 accatcctat aaattggcag agagtaacct gtggcacata accctattcc ttcagcgtga
     6001 agcgctttga caaacaaatc tctgtgaatt ccccagcatc tctccttttt atatttaaat
     6061 acatataagt gatgagagtt tttggttata ttgggatctt catacagcgc atctataaaa
     6121 cctgtttcct taagtctctt tgtaagatat gcagcattgg tcattctctt ctctatctcg
     6181 ccatccagtt tattaagctg tacatcaagt attactgcct gccattctgc cattcttgca
     6241 tttgtaccaa gaacaggatg ctgataccat atattgctgt cataatctct gccgcagttg
     6301 tgtacactcc atatggattt gtacaggttc tcgttatttg tagttatagc gccaccctct
     6361 ccacagcata gatttttact tgcttgaaaa ctaaaagaac ctacgtctcc tattgagcca
     6421 accttcctgc ctttccattc cgagccatgg gcatgagccg catcttcaat aacatagagt
     6481 ttgtgcttcc gggctacatc cattatttta tccatatcac atggccttcc gcctaaatgt
     6541 actgcaatta tggcttttgt ccgctgtgat atatgctgtt caatatttgc aggatcaagg
     6601 ttataagtgt tttcatctat gtctgcaaaa accggagttg ctccagcaaa tgtaatagca
     6661 gatgcggttg ctacaaaagt atacggaggt aatattacct cgtcaccacg ccctatatta
     6721 agtgctctta gaataacttc aaggcttact gtaccattag gtgttgcaac agcatatttt
     6781 gcattgcaat aagctgcata tcttttagcg aatttttctg tctcagctcc taatgttccc
     6841 cactgtcctg aattgagtac tctcttaagc cgctctaatt cttcttctcc actcttgggc
     6901 caggaagtaa aagacttggt tcttaaaggt tccccgcctt ttattgcaag tgttgacata
     6961 gctttccctc cattttcatt gatattttaa cgttttattg atattttaaa ggtttttatt
     7021 tcttttcata tctttttctc tattgaaaag cacccttagt tatgaaatac tcttaagcca
     7081 tctgtttcta agtaaaatgc ctgcaccaac gtgggcattt tttacaatat ggtttgataa
     7141 aaatatttcc ggtatatcct gtcgtatcgg tagtaacatc tgagtgagca ttgtctttat
     7201 gccttcaata aaaaaatcat gatccaatcc ggcatatctg cattcaatta ttatggcatg
     7261 gggatctata aaccatataa cattatataa agctgaagaa atcctcttgc agaaatccat
     7321 caaacgccgc ctgaaatcat gaaagttgtc tgtgtctgct tgatagtctt ccagcatctt
     7381 cattatatct tcatcactac cagtaatatt atcattacca gctacttccc tggcaaaagc
     7441 ttttagtgat atgagttcct caaagcaggt gttttcgtct gcaagtacct gaccaatatc
     7501 cccggcaaat gaaaataacc cgcgtagtat agatccatta actgatatgg ctccgccaac
     7561 cccttctcca atatatgcgt aatatatgat ttttccggta taatcaggaa ttgttgttat
     7621 attagcctga gcagcaaact taacatcttc atctataaag atgtcaagga tataatcttc
     7681 aagaatattg gtttcaagaa tattgtcttc aagggtatca tcttctatgt atttacctct
     7741 tattaatgtc tcgctgatta tagattttat gtttagctgc attaattcag gtatacgttt
     7801 atttattaca atgtcatttt ccacatcata aggtcccggg acaacaactc ctatacttat
     7861 agtttcatca accgggaagt gggtatttat atacttatca gtgattaata gaaaatcttc
     7921 caaattatct gtatagttgc gttcaaagtt gtatctccat tctttgaagg tattgtgtat
     7981 tgtcaaatca aggtttaata tagtaaatga aaattccaat gatgtcagat caattattat
     8041 aaaccttttt atatctttat taatagaaag aagctcagct ttccttccta cagtgtttgc
     8101 ctgttctttg tctacatgat gtatgatacc gaacctgtcc agatgatcta ctatgttgca
     8161 tacagtcatt aaacttaagt ttgataatct ggcagcatca ttacgggtta ttttatcatt
     8221 gttgacaatg atatcaaaca acaatttgat gttttgagct ttaatagtgt ttaaaccaat
     8281 ctgtttcatt tttcttcatt cgcctttcaa caatctgtaa aagtttaatt tgcaaagttt
     8341 acaatgattt tattttcttc gagtatttat ctattaatga tttattgatc ctgtcgccgc
     8401 catctacatt gctgctgcta aatacttcgg gtactacatt tttaccaagc attatctcta
     8461 ttgcttcgca tataatggcg tgcagaagca tggcacccat aactgttgaa gtgggtccaa
     8521 cattaccgat acggtcaaac tccatagatg cgtcaccaat gcttccacaa ttatcaatta
     8581 ttatatcggc cagttcatat agaagcttcc cgctttcatg cctagatgcc acactttttg
     8641 aatgtttaag atttgttaat gcccatactt tcattcctct ttttttagct tcaatggcca
     8701 tttcaatact gacagtattc ctgcccgagt tagatgctat aattatgaca tcatcttttt
     8761 gcacaggata gttatccagc agagcttttg catagccatg cagcctttcc agcgaagtac
     8821 tttttactgc tcctgcatga agcattaatc cctcatccaa tatggggttc accctgacaa
     8881 gtcctccagc tctataaaat atttcttcag ccagcatatg ggaatgcccc gtgccaaaag
     8941 tgtatatcat tcctcctgaa attaaacact gtgaaatagt ttttgcagct tcttccaatg
     9001 cttgtatttg ggtttcttta acttccttta taagtctttc tatgttggat atataatcca
     9061 ttgcttttga catatatttc tccttcctac atctatatat aacacaaaaa tgattatact
     9121 ttgagattat ctaaaaattc ttgagtaaca gggtatcccc tttttttcat accatacaac
     9181 acggctccta tttccggtgc aagctcaggg aaactaattt cagcttttgg atataaggta
     9241 tgaacttgac taataaaaga gcttgaaatg tatggactgt tttgaaatac accaccatac
     9301 atgcctacaa tgcagtcgtc aatacgaagt atatccataa gtctgcagac atattgaaca
     9361 agaatggcca cagctctatc aactatagaa gcagcaatac tatcgccctc ttttgcacat
     9421 ttctccacct ctttggcaaa ttttgcaagt tttgagtttt gcattggtgg attataaaga
     9481 acatcaatga gctgacgata gctttggacg ttaaaaaagt ttgtaactct tttttcaagt
     9541 atagtttttt ctcccgtact ttcaaagctt cttatacctg cctttattcc ttcaatagct
     9601 atatgatatg cgcttccttc gtcttgaagt aaatatcccc atccccctaa aatatgtaat
     9661 ttttctgttt gatcaactgc aatagccatt gaacctgtcc cgctgactac cataatgccc
     9721 gatttgccca atgtcattcc catcagtgcc atatatacat cactttgcat ttctattttt
     9781 tcaggctgaa aaactttacc tgcaaatgcg attattacgt cttcagcagg ctcatcatct
     9841 aaggaggaca tacctatgga aatgaattca taatctgaga ttccgcattc atctataagc
     9901 ctatcaatag cttgctttaa gttacttctt gctgcagaca tgtttgtagc atagaagttg
     9961 gtcccttctc ccacggtctt gcctaatact tggcctttgt cagttacggc tataatgttt
    10021 gttgatgtac ccccgccatc aacaccaaca aaaattctat ctgccagatc cctcacctct
    10081 tttttagtat tattctagta ttattttggt attattatag ctccaagtta tgatgctgct
    10141 ttataaaacc aatttataaa tagactttat aaacttcttt tattatatca tactataaat
    10201 ttttttcaag ataaatcatg aagaacaatt gctagtttag acagcttgac aaaagtgctt
    10261 tatacaccat ttgcacttga agaatatgta agacagcata gtcattacag agttgaatga
    10321 gtattaacat gcaaccaacc cggttccctt tctataagaa tatccgatgt tgctttaagc
    10381 atatctgatg ccgcttgttg tgcaattttc agtaattcta tatccttata caggtttgca
    10441 atcttaagtt ccggtattcc atgctgcttt gtcccgaaga attccccggg acccctgagt
    10501 tccaggtctt tttccgatat tataaagcca tcacaggttt tttccattac ctgcatacgc
    10561 tcttttgcaa ttggagaatt actttcatta tacaaaatgc agtaggattg gtcttttcct
    10621 ctcccgaccc ttccccttaa ctgatgaagc tgtgccagtc caaacctttc tgcgttttct
    10681 acaaccatta ttgttgcatt aggaacgtcc actcctacct caacaacagt agttgatacc
    10741 agaatatcaa ttttcccttc aataaagtcc ttcatgacct gttctttcaa gtctggtttc
    10801 agctttccat gaagaagtcc tactttcaaa tctttaaaat cctttttggc aatatcttca
    10861 gccagggtaa ttgttgacat tgcatcaata tcatcagaat cttcaactaa tggacatatt
    10921 atatatacct gtctgccctg ctctgcattt ttacggataa aattattaat tctttccctc
    10981 atgcttttat ttactatata agtcttgaca ggtgttcttc cgggaggtaa ctcatcaatg
    11041 actgatatat caagatctcc atagagtatt agggcaagag ttctggggat aggtgtagca
    11101 gtcattacaa ggatatccgg gttttcccct ttttttgaaa ggcaggctct ctgtcttaca
    11161 ccaaatctgt gttgttcatc agtaatgacg agccccaggt tataacattc aactccatcc
    11221 tgtattagtg catgtgttcc tataactata tcaatatcac cattttttat tccttccaaa
    11281 actttcgatt tttcctttcc ggttatattc cctgtaagca atcccgtatt aacattatat
    11341 ttctggagaa gccctttgac taaccgataa tgctgctcgg caagtatgcc tgttggagcc
    11401 ataaggactc cctggtaacc attttcaact gccttaagca aggcaaggac agctacaata
    11461 gtcttccctg aaccaacatc tccctgaatc aatctattca taaccccgct gctttccata
    11521 tttgtttcta tatctttaaa taccttcagc tgagcattgg taagttgaaa aggaagagat
    11581 ttaaccaggt catggacgtt ttctcctttt ttaaatgaaa ttcccttttt accttcatta
    11641 aaagattttt ttacagctaa aaggcttagt tgaagcgaaa acaattcttc aaataccagc
    11701 cggtatcttg aattattcaa agcttcatta tcatggggaa agtgaatgtt ttgtattgca
    11761 taatttattt cactcaaaag gtatttttct cgtatgtttt ggggtagtat ttcagggatt
    11821 atgccatcaa cctgcttcaa cccttcttta gcagcagacc taatgactgc ctgagataat
    11881 ttaaccgtgg tagaatatac aggcactata ttgcaaaagc tatccttggt cttttcatga
    11941 tctcctgact tctcataaac cggggtttgt acttcgataa aactgcctgc tcttttgatt
    12001 ttcccataaa aaaaatagct ttcacccggt ctaaaaacat ttttaagata tggctggtta
    12061 aaccaaactg cataagctat gcctgtatca tcagatattt caattttctg aattgtcatc
    12121 cttgctccat ggctggtttc tcttaatttt gaagtaattg tacctataaa agcagagttt
    12181 tcttcattct gtaaattgtt tatctttaca agtctgctgc gatcttcata atctcttggg
    12241 tagtactgga taagatcctt aacagtgaaa actcctattt tgtttaaaag tttctccctt
    12301 gccgcgccta ttcctttcaa acagcttata ggtcttttta atatatcttc agaattatta
    12361 ttcataaaaa taaatcatcc acctttgctg gtattataac catccctatg tttctgtact
    12421 attatactac aaaaatagtt ttaaaaatag ctgcaaaaaa cttgattatt aatatgcata
    12481 tatgttagaa tattagttgt ttgaccgcaa cagagggaaa agttgatgta cattttctcc
    12541 attgaaggag gtgcgatatt aaatggcaaa atgttttata tgtgaaaagg aaacaacatt
    12601 tggaaaccag gtaagtcact ctataagaag aacttccagg tcatggaagc ctaatataaa
    12661 aagagtcaag gctttggttg atggttcagc taaaacagta tatgtatgca caaggtgctt
    12721 acgttccaac aaggtaacaa gatcagttta accttattaa taatggatct tggcttaaat
    12781 aattttgcag aataaaaaaa gtgtgaagac acactttttt tattctatgg cagtataata
    12841 tgtgctatgt tcactgtttc atctcatacc caataccttt ttaagtacag aactaagaaa
    12901 tttaggcata cgtacaacaa gtatcttcat ttaaatacac tccgttcaat ataatctctt
    12961 ccttatcaat atattcaaat aatttttttt ggtgcatgtg agtgcatggt gtaaacttta
    13021 tgatatgtgg acttctataa gtgcgttttg ctgaataaaa tatttagtag atttaatact
    13081 aatatgccaa ttgggaaggc gatatcttac gggataattt ttgatagcat cgggaaggta
    13141 gtactttcca taagcaacat aacaaagtag tattaaataa aattaatatg aataataggc
    13201 aggttatgca aatgattgtc cttttctata gagatgcaaa aaggaaggct atattttcat
    13261 tattagtggt tgctttatgg gcttttatgt attacatatc cggactgttc tttgaaaaag
    13321 tgctcgtttt aaatataact attggcgata tgttttcttt ttcttgttca ccagctctga
    13381 agttgggaaa tatgtatgtg catcatcttg ctgatgatgc cattacagta aattcaaaca
    13441 atgtaggacc ggaaagccag aaattttcaa cttacaattc gttgaaagga aaattcagtt
    13501 ttgaatatcc ttccatattt actctcagtg aaaaagaatt cccttcatca gagatacttt
    13561 atcatattga cttcaatgat aatgaacgca tagtacatgg tttcgttcag gtgtgggacc
    13621 tcccttattc acttaaagat ttccttgata aatccaaagc caatagtact ctgaattata
    13681 aaagtttcaa atcgacagaa gctgctattg acgataaaga aggttattta tgggattaca
    13741 ctgtgttagg tggtaacgaa aatataaaat ataaagcatt agaatatttc tttaaaaaag
    13801 gtagtaggat gtatagaata agcatgtttg ccccagaaag ccactggagt gaaaataagg
    13861 taaatatttt ctggggcatg gttaagtcat ttaaagtgtt tcgcaaagaa gcatcagata
    13921 ctgagatttt gtccgtaatc acaatgctct cttatagggc acctggtgca ttggggtttt
    13981 ctggcagtgc atattgctct gccatggtat accagctggt ggcaaaactt tgtccactct
    14041 tgtttgggta ctatttccat taaatcatat tcaattttct ccggatgatc atttttggtt
    14101 aatcctatcc gtttggacag ccttgcagca tgggtatcaa taacaacccc aggtatacca
    14161 aaaatatccc ctagaactaa attagctgtt ttcctaccca ctcccggaag ttttagcata
    14221 tcttcaagat tatcaggcac cttaccatta aaatgctcta tcatcatttt acagcaattt
    14281 gttatatttc tggctttatt cctgtaaaat ccagtagatt ttatgtcatt ttcaagttca
    14341 ctctggtctg catttgcaaa atcttcaacg gtcttatact ttttaaataa atcttttgtt
    14401 acaatgttaa cccttgcatc tgtacattga gccgctaact gggtagcaac aagaagctgc
    14461 aaagggtctt tatagtgcag tgaacatgaa gcttcagggt atagcttgtc aaatacatta
    14521 ataatttcat taactctttt tatgtttttt tgcctgtttt tttcttctac tttatttccc
    14581 attttgtttg gtgttacctt ttctttcgtt attttctttt ccattgttaa actaatttct
    14641 tttcaatagc tatatagtat aactccatat atttctctgc ggatctgttc catgaaaagt
    14701 cccgggtcaa agccctttct actagcattt tccattcttc aggtttgcga ttatatagag
    14761 ataaagctct ttctatagtt atatacaatt catcagggga aaattcggta aaggagaaac
    14821 cattcccatt ttccctatct tcatcatagt caataattgt ctctgcaaga ccccctgttg
    14881 accttacaat agggatagta ccatatctta agcttataat ttgacctaag ccgcatggtt
    14941 caaagcgcga tggcatcaaa aacatgtcac agcctgcata tattttctgt gccagaacag
    15001 cattaaagcc tataaatgca ctcatttttt caggatacct gttttttatg cctgtaaaga
    15061 tgctttcata atagggatcg cctgtgccta aaagaatgaa ctgcaaatca tgcttcatta
    15121 tgtcatcaat tctttcaatg attaagtcca gtcctttttg tcctgacaac cttgatacca
    15181 aaccaatgac cggcatatcg cctgcaggga gacccatttc ctcctgtaaa gcatatttat
    15241 tttccttttt tgcttccaaa gattttatat cataattctt atatattctt ttgtcggtag
    15301 aagggttaaa atcctcatag ctaataccat ttacaattcc atatagatct tttcccctct
    15361 ttctcagcaa gccgtccagg ccttcaccgt attggggagt ctgaatttcc tttgcgtaac
    15421 tgctgctcac ggtattaata atatcagcat atacaatgcc cgctttcata aagttaaaca
    15481 ttccgtaata ttctacttta tcaggtataa acacttcccc tccaacatta aaaagacttg
    15541 ttacttctct tgggaaattg ccctgatact caaggttgtg tatagtataa actgaagaaa
    15601 ttccactata aaaagatata ttattatatc tttctttgat cagcatgcaa atcggtcctg
    15661 tatgccaatc attgcaatgt attatatcag gttgaaaatt aatcttagga agcatttcaa
    15721 gtactgaatt gcagaaaaac gcaaatcgtt ctgcatcatc aaagtgacag tatataccat
    15781 ctctgtcaaa gtaatcataa ctgtctacaa aatagaaggg aagactggct tcagtttccc
    15841 ttattataca tgttttaaca ttatccccca ttttaacggg gaaatcaact acatatttca
    15901 ttggactctt tattgcttta tatcccggca taactaccct tacatcattt ccacctgctg
    15961 caagtgcttt aggcaaagaa cctgctacat cggcaagacc ccctgttttc gcaaaaggcg
    16021 acacttctgc tgaaacatat aatattttta acttcccttt atgatgcatt ttttaatgcc
    16081 ctccagttat tatgttttca tcctttttat tgctcatcaa atagaggttt atcctctcca
    16141 agttcaactt ctatagtcaa agtcttgtta tctctatata tctcaagctt tacaatatca
    16201 ccgggcttat acttgtcttt aatagtattt aactcatcaa ggctttttac aggtgttccc
    16261 tcacatttta caactatgtc tccaactttt atacctgctt tataagcacc actgaaaggt
    16321 attacttcct ttacaagtac gccttccggt actttgtagt atctggctgc ttctgaatta
    16381 aagtcgggat ctgctctcaa tcccaatagt ggtcttcccg gaacatattt atgctcaatg
    16441 aggttttcaa atatatcctt ggcggtattt attggtatgg caaacccaat accttcaaac
    16501 ccctgggcaa ctactttaat actgtttact cctataacct ttccctcaga gtttacaagt
    16561 gctccaccac tattacctgg atttatagcc gcatctgtct gtataagttt aaactcatag
    16621 ccattttcag tctggactct tctattaaga ccgctgataa ctcctgctgt aactgagccc
    16681 atgtattcaa ggcctccggg gtttcctatg gtaacagcaa gttcacctac ttcaagagca
    16741 tcagagtccc ccagttcagc ggcaggaagt ccggttccgt taattttcaa gactgccagg
    16801 tctgttctcc agtcaactcc tacaatttct gctttgtagg gtttatcttc ctggtttggt
    16861 aaaaatacct ctacagttgc actgccgctt actttatttg tcctggaatc atatgccttt
    16921 tcaattacat ggaagttggt taagatatag ccgttatccc ttactattat acctgaacct
    16981 tctcccttat cctgctgttc gccaaaaaag aagttttgaa cctttgcagt aacccttatt
    17041 cctacaatag acgggctgac tttcttggca atccaggtaa caggtgaatt ggttttctcg
    17101 atttcaatct tatatgattt tccagcatcc tgtccgtttg caggactatt tgaagcaaca
    17161 tttttacctg tcatcctgtc aaaatagctg ctaaactcag gctgtatggc aggtgctata
    17221 aactggaagg ctgaaaatac taccccccca cccaggattg aactaataag aactattgct
    17281 accagctgaa gcatattaag tctatatcct tttttgcttt tctttttatt atagctttcg
    17341 ctgtaataaa aaggtgaacc ttgggaatta ctcctgccgc tataagtact attactataa
    17401 tttatattgc tataactact gctattatta ctattgtata aagagccgga agtatggtca
    17461 ctattttcag aaaacttttc tgaaccatcg tcattaattt ttttaatatc atctttattc
    17521 agtctgtaat tgttatcatc atcaaacatg attacccctc cttgcaatta ttttcattac
    17581 ttattaaaac cttaaataaa tatgtttttt taataatgca gttttataat aagtaattta
    17641 tccttcacta accttacaat tatattttaa ttatactttt ttaagtaagt gtgaataatc
    17701 tgttaatttt catactggat tatattaggg attcatctgc ccgctttaaa gtgaaatgga
    17761 aagttgtacc tttacctggt tcgctttcaa cccatatttc ctgaccatgc tcattaataa
    17821 tattctttac tatggccagg cctaggcctg taccaagctt atctctgctt ctcgatttat
    17881 cggttttgta aaagcggtcc catatcatgt ttaattcttc tttatctatt cctatacctg
    17941 tatcagctac tgatataatt aatctatcag catctattat tgtgtttatg ccgataatac
    18001 ctttattagg agtgaatttc acagcattgt gtatgagatt tatcaatact ctctcaatag
    18061 catccaaatc agcactaaca aaggtctcct cgtcaaagtt taccgttacc tccagttctt
    18121 tttctgttat taggtgctct agtttaataa tgcatctgcg gattaactca ttaatattaa
    18181 aatttgaaaa cattaaatga atttccccgg attccatttt agccaggtct aatagatcat
    18241 tcacgagtct gttaagtctc ataacttcat ctcttacgat attcaggtaa tccttctgtt
    18301 tttccatggg aattgtccca tccagtatac cttcaataaa ccctttgatt gatgtcatag
    18361 gagttcttag ttcatgagat acattagcta taaaccctct tctcatttct tctagattct
    18421 ccaaagcgac agccatctga ttaaaactcc ctgccaattc tccaatctcg tctcttgaat
    18481 ttatgttaag ccgtttattg aattccccgc ccgatataat ttttgctgca ttgcttatct
    18541 gcttcagagg atttgttatt ctacgagaga aaaaatacac aaggaatgtt gaaattaata
    18601 ctgatacaac taccgaaatg agaaagaacc tgtaaactgt agatctggcc ttatttattt
    18661 ctgtaattgg agtgcttaaa tacacagccg ctacaacatg ttcagcacca gcagtatcaa
    18721 catatttaaa tggcttctga actacaagcc aggaccagcc tgtttctttg aacaatccat
    18781 aaaaatcgcc cttttcaatg acaggatttg taccagacag tacttttttg tattgtcttt
    18841 catcaggaag tatgtatgtg ccctgttcat atttaagatg gttgaggatt ttactgtctg
    18901 ttaattttaa ataaggactt gcaaaaacaa taactccctt atcatttgtt atccatatat
    18961 aagaaccggt attttgtctg tatgaattca aaacgtaatc caacatgact tcagctacag
    19021 gattttgcat attttccagg taaaatttca aatatttgct gatttcttct ccactggcat
    19081 ccagtgtcct tattttttct tcagaaacat acttgccaaa ggaataatac aaagttatcc
    19141 cggaaataga aaagctgaaa attattatag ctatgaacat tgccacaagt ctgctgaaaa
    19201 tcgatttaaa catcatttca cctcaaattt gtaccctaca ccccatacag ttttcagctg
    19261 ccatacctga tttggcaaat ccagtttctc ccttaaccgt ttcacatgaa catcaaccgt
    19321 ccttgaatcg ccataaaaat caaaccccca tacatgctcc aggagctgtt ctctggtaaa
    19381 caccttattg ggattggaag ccaggaaaaa cagcagttct aattcttttg gcggcagttc
    19441 catttcattc cccacaattt tcacagtgta attggtctta ttaataacaa ggtttgggta
    19501 cactacctct tcagtatcaa gctcctttct ttcatatctc cgcaagacag cttttaccct
    19561 cgcaacaagt tccttaggct caaagggttt taccatatag tcatcggctc caagctctag
    19621 ccccaatacc ttatcaaaag tttccccttt ggcagtaagc attataatag gaatattact
    19681 tatttttctg atttcacgac aaacctgcca tccatccgct cccggaagca tgatatccaa
    19741 aattacaata ttaggagcaa atctgctgaa ctcctctatg gcttttttgc cgttatatac
    19801 agcgagtacc tcatagcctt ccttctccat ataaagtctt ataagctcac aaatatttac
    19861 atcatcatct atcactagta ctttagtttt attaatcata ttaaagctcc tttatatact
    19921 ttctttgttt tctgctctta ttaaatctat tatatcattt tctttcaaaa ttttaactaa
    19981 ttcttttata ttagctatag aatctttaat aatgattcct ccccttgcta aatcgacaac
    20041 ctgatgaaaa tcggaaccag atatcattct caactggttt tctattgcaa aattatatgc
    20101 cagatcattt tgtgaattat gtctcgggtt tccgttatat gcttcaatac catcaagcag
    20161 ttcaggttct tcaatagttt gcccttgcct ataagggtgt gcctgaaaaa ttaaaatatt
    20221 tttatttgac atataagatg catttgatat aaaatttctg aactccccta gggttaagtt
    20281 gtaaagctcc gggttatctc ttaaaaactc ttcatctatt ccataaacca aataatcgtt
    20341 tggattatcg ttaaacctta tttccatacc caaaagtaca ttcaaaccta tactggcacc
    20401 ttcttttaaa gcttttttat accccttcag gtaatcttca attttcattt gccaattacc
    20461 gcccgtgctt ctatcaaaaa agcctttata ataatgatct gttattacta ttccggcata
    20521 tcccgcttct ttatacaatt tgactacatc aattgcatct accttcccac aagggcttgt
    20581 ttcagctgtg tgtacatgaa tatcatattt gttcatattt atgttccttt caatgtaata
    20641 tataatttgc aacgtttttt cttgacacat ttttaatatt tatgatacta ttaaattgtg
    20701 aaaatgtaaa tgcaatgaaa gggaaaagta agttgtaata atcttcacag cgaatcgggg
    20761 atggtgaaag cccggtagta agttgcaatt gaagttctcc ctggagctgt tggctgaatt
    20821 tatatgtaat acttgtaggc cataccggag gttccgccgt taaaaggaag aatgtatcgg
    20881 atttgttcca taacaacggc tttttatgct tgtttttcaa gtataattgc tgttttcagg
    20941 tactgtcccg tacattaatt gagcgggctt ttgccaattt gggtggtaac gcgtggaata
    21001 tacactcctc gtcccttaac aggacgggga gtttttattt taatgtgtac aatacccata
    21061 agcaaattta tgctgagtta aaccaataat acagatattt ttatatttca ggaggttttt
    21121 tgtatgatag ttgtaatgag tcaaaacgct acaaaagagc aaattataaa tgtagaaaga
    21181 aagctcctag agttgggttt ctcaactcac cccatctatg gagagataaa gacggttatt
    21241 ggcgcaatag gagataagct tctgctgaat acacaagcga taaccactat gcccggtgtg
    21301 gagaatcttg ttccaataat gaaacctttc aaacttgcca gccgtgaatt gaaacaggaa
    21361 tcaacaattg ttgaagttgg tgatgtgaaa atcggaggtc cggaaattat tgtcatggcc
    21421 ggtccctgtg ctattgaaaa cgaagaaagc ttcatggata ctgcatttaa agttaaggag
    21481 acaggtgcga agattttgag aggaggagca ttcaagcctc gtacttcacc gtactcattt
    21541 caaggtttag aagaagaagg tctgaaaata atggctaaag ccagggaagc aactgggctg
    21601 aaaattgtaa ctgaagtaat tgatacccga gatgtggaaa cagtatcatt ttatactgac
    21661 ataattcaaa ttggagctag aaatatgcag aatttcaggc ttttgcagga agcaggttct
    21721 acaggaaaac caatactgct taaaagaggt ctggcagcaa ctattgaaga gtggcttatg
    21781 gcagccgaat atattctatc ctccggagat agtaatgtta tcctttgtga aagaggaatt
    21841 agaacttatg agacagcaac acggaatact atagatatga gtgcaattcc tgtagtaaaa
    21901 gagctttctc atctccctat aattgttgat ccaagccatg ccacagggac atggaaatac
    21961 gtggcaccgc tttcaaaagg cgcaatagca actggtgcag acgggttaat tattgaggtg
    22021 cattcagatc caacttgtgc actgtgtgat ggtccgcaat cattaaagcc ttctaaattt
    22081 tcccagttaa tggaggaact ggagccggtg gctctggctg tgggacggaa attgcgatga
    22141 tgataaagcc cggggacact gggcgcatat tacatttctt cataaataaa aatgagcacg
    22201 gtgtccccaa taaattaccg ttcactgtaa agccttatat aaatctgttg gtattttctt
    22261 actcttttta tgaatgtatc agtttccctg aaaggtatta catccagctt tttacctgtt
    22321 ttactataag tattgttcct aagccactca tttacattac cgctgccacc attataagct
    22381 gctaaaacca gatcaatgtc gtttttaaac tcaaccataa gctgttttat ataccagcat
    22441 ccgattctta tatttgtttc aggatcataa agatcctcta ttttgaaaga tttaatgtcc
    22501 atactttcag ctccccactt ggcggtgttt tcggtgatct gcattaaacc catagcatcc
    22561 tttttagatt ttgcataagg gttgaaactg ctttcagctt taataactgc aaatacaaga
    22621 tacggatcga ttccattaaa ctcggaatag ctgaaaacaa gttcctgata ttttaacggg
    22681 tatattcttt taataatggt agtagagttg tttataatta atactattaa taaaaataca
    22741 acaattatat aaagtatgta cttgtattct tttacattta ttacatttat gttaatacat
    22801 ctctttttat tattcaagtc cgatcaaccc acccaaatca tttttgcgtt gtatatattc
    22861 gagataaact ttatctacta aacctatctc agtttcttta tattttctaa acctgcccta
    22921 atttcttttg aacataaagc ctggctatgg taccttccag ctcttccaat gtaccattat
    22981 tgataataac ctcattagcc agccgcaagt actcttcatc acttttttgg gattgaatcc
    23041 tgttaactgc ctcttcatat gaaattttat ttctttccat aactcttttt attcttgttt
    23101 ccttatccgc agttacaacc cacacttcgt taacaatatc aaggaagcca tgtttaacag
    23161 gtataggtac atccaatacg gtaaaactgg ttttgttctc atatcttagt ttttcaagat
    23221 gtgaaataag cctttttact acatatttat gcgtgattga attcagtata tcaagcttct
    23281 ctttatcgac aaacacaatt tccgcaagtt ttgttctgcg gagttcacca ttatcattta
    23341 atattcccgg gccaaaataa tccaccagct cctgcagagc aggttcaccc ttggctgtta
    23401 tttccctggc tattttatca gcatcaatta tttcagctcc caggtcactt aaaatacggg
    23461 ttaccgtgct cttgccgctg ccaatcccac ctgtaactcc aataaccttc actatatttc
    23521 accctcttat gcaatgtatt tcacctatgt ttctgtttcc tactttgtct cgtaccagtt
    23581 ttctcctgtt ttgatatctg cctttaaagg tacattaaga cttactgctg tttccatgca
    23641 ttcctttaat atctttatca cttcattttt ttcatctaca tgggtttcga taattaattc
    23701 atcatgcacc tgcaatatta atcttgactt catgttattt tcttttaact gattgtaaac
    23761 tttaaccatt gctattttta tgatatccgc tgcggtaccc tgaatgggag catttaaagc
    23821 aacacgttgt ccaaaggatc tgatgttgaa attttttgat aataactcag gtagatacct
    23881 ccttctattg aacaaagttg caacatatcc cttttccttt ccctcatcaa taacatcctt
    23941 catatatttc ctcacacccg gatatttatc cagatagtca tctatatatt tcctggcttg
    24001 ttttcttgta atccccaaat cttttgccaa gctgaaatct cctattccat aaattattcc
    24061 gaaatttata gctttagacc tggacctcat caatggtgtc acctgatcca gaggaacatc
    24121 aaacactttt gaagcagttg acgcgtgaat atcttcattg tttataaatg cagatataag
//
LOCUS       DAOSDD010000037        23855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_947_length_23855_cov_4.746975, whole genome shotgun sequence.
ACCESSION   DAOSDD010000037 DAOSDD010000000
VERSION     DAOSDD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23855
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_23855_cov_4.746975"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK733_05210"
     CDS             <1..360
                     /locus_tag="PK733_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09974.1"
                     /translation="LQLKSCELIITILYKEVILMSMPTIPDIKPKIEISFEETIKLLL
                     ASIALEELSLAHIMNAEAEKVQEVIKLGGCNKFDELLCINKSVEKTLKDVIKKEMLLE
                     FKFENILELMGKGFKDC"
     gene            548..805
                     /locus_tag="PK733_05215"
     CDS             548..805
                     /locus_tag="PK733_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09975.1"
                     /translation="MKEREINASFIGKKIIENAVKLDESLVDILKIEVRRLKQSIKSN
                     NNLDELQRTNNLIKNIIIALTITDERIQTGIDLCMNNSETE"
     gene            940..1770
                     /locus_tag="PK733_05220"
     CDS             940..1770
                     /locus_tag="PK733_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOJ09976.1"
                     /translation="MDLATLIGIISGIICTAYPIFDGGYPKTFLNIPSLMLVVGGGIA
                     STLISYRIKEVTRIFQVVGSAFSRKEVDLESTIRLLVELSQKARREGLLALESEQDNI
                     EDSFIRRSVQLIVDGVEPEMIKESLDLELDNMALRHERGQSLFRTMAVLFPAWGMIGT
                     LIGLINLLKALDKPENIGPAMALALVTTLYGSLLAHFICNPIANKLSLKSKEEIQQKE
                     MIIEGILSIQAGENPRIMEHKLKTFLSSEQRELYEAKNEDLFQQKDNKSNSSSRIVTE
                     "
     gene            1785..2588
                     /locus_tag="PK733_05225"
     CDS             1785..2588
                     /locus_tag="PK733_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOJ09977.1"
                     /translation="MSRQSKRHRTDSSSDSAGDWMNTYADMVTLLLTFFILLFSMATV
                     DKQKFVELANSLRSAFMHENGGGDLYESNRGSELINIYDEINAVDKVVSLVNNDYEGG
                     ETDNNVDRITDGNEVEYNTDDLKAEIEKAIEEMGLEEYVKIIERKDFLILRFDSVILF
                     DLSSADIKSSAKTVLEKLGQMLNKMSNEIIIQGHTDNLPINTGQFPSNWELSTRRATN
                     VVHFFINECSIAPSRLTATGNAEYKPVKPNDTAENRQQNRRIDIVVSKD"
     gene            2901..4880
                     /locus_tag="PK733_05230"
     CDS             2901..4880
                     /locus_tag="PK733_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOJ09978.1"
                     /translation="MEKNKSKNTYGNESISSLKGPDRVRLRPGVIFGSDGLEGCQHAV
                     FEILSNSIDEAREGYGSVIEVTRSKDKSIMIRDYGRGIPLEYNPKEERYNWELVYCEL
                     YAGGKYKNYLGENYEFSLGLNGLGACATQYSSEYMDVTVFRDGYRYDLHFEKGCNIGG
                     LIKKKSDNSQTGTIQKWRPDIDVFTDIDVPLSYFQEVLKKQAVVNSGLKFILYDEESD
                     LTFEFFYEKGIIDYVNEINADKGFTGVEFYETTARGRDREDKPEYKVKIEAAFCFNNE
                     INLLEYYHNSSFLEYGGSPDKAVKAAFVNEIDKCIKARNKYNKDESKITFSDIQDSLI
                     LVTNSFSTITSYENQTKKSITNRFIQEAMTDFLKQQLEVYFIENKLESDKIIEQILLN
                     KRSRETAEKTRLNIRKKLSGNIDISNRVKKFVDCRTKDINRRELYIVEGDSALGSCKL
                     GRDAEFQAIIPVRGKILNCLKTGYEGIFKNDIITDLLKVLGCGVEIKSKHNKELNTFD
                     MGNLKWDKIIICTDADVDGFHIRTQVLAMLYRLVPTLIQEGKVYIAETPLFEINAKDK
                     TFFAYDEKEKINIISGIKGKYTIQRSKGLGENDPDMMWETTMNPETRRLVKVVPEDEE
                     KTREVFDLLLGDNLQGRKDFIEEYGHMYMDMIDVS"
     gene            5009..7195
                     /locus_tag="PK733_05235"
     CDS             5009..7195
                     /locus_tag="PK733_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOJ09979.1"
                     /translation="MNSNNLIEQHISNTLEENFMPYAMSVIVSRAIPEIDGFKPSHRK
                     LLYTMFSMGLLTGNRTKSANVVGQTMKLNPHGDMAIYETLVRLSKGYGALIHPFVDSK
                     GNFGKQYSRDMRFAAPRYTEVKLDKISQEIFRELEKNAVDFVDNYDGTLKEPVLLPTT
                     FPNILVNPNQGIAVGMASNICSFNLQEICEATVQYLNNENTDILKYIKAPDFATGGRL
                     IYNERDIREIYETGRGGFKVRGKYRYDKKNSCIEIFEIPYTTTTEAIMDAVIQLVKSG
                     KIKDITDIRDETDLDGLRLTIDIKKNTNADDLMNKLYKLTPLEDTFSCNFNILINGVP
                     RVMGIKTILTEWIAYRINCIKRLTLFDIERKSEKLHLLLGLQKILLDIDKAIRIIRNT
                     EQDSQVIPNLMKAFDIDKVQAEFVAEIKLRNLNKEYILNRVGEIEQLQKEIGELKDIY
                     GSENKIKKIIIKQLTEVTKKYGQPRKTEIIHEEFVEEITEEHFIEDYNVKIFLTEQSY
                     IKKISLVSLRANPEQKLKDDDHIIQEIDARNKDDLLLFSDKTNVYKLKVHELEDLKAS
                     VLGEYLTNKLELDEDEKIIYIVATGNYKGYMLFSFENGKTAKIDMNSYATKTNRRKLL
                     NAYSGLSKLIHIMYLENDLELVAYSSIDKVLIFNTACINPKTTRDSQGVQVLKEKKGS
                     WMKEIKTVEQADLSDPEYYRTKNIPAVGCYLKKEDKLSEQLELKLE"
     gene            7214..8593
                     /locus_tag="PK733_05240"
     CDS             7214..8593
                     /locus_tag="PK733_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOJ09980.1"
                     /translation="MIPKDIIEEVLSRALSLGGDFAEVFIEDRVNNSLRMVNGKMEDV
                     VSGRDYGAGIRVMKDFRSVYAYTNDTSRDNLLDTAYKAACALKYTKQDITVNLVERVS
                     ANIHPVLFVPSSITNMKKASIVKTAYKVAKDYSHEIIQVAAGYGDVDQKILIANNEGL
                     FIQDRRVRTRLFIQSVAGDGIENQTGFEGPGKSMGFELFDVIDVEGYAKEASRIAVTM
                     LHAEPCPAGRMPVVIDNGFGGVIFHEACGHALEATSVAKDHSVFTGKLGMKIASDKVT
                     AIDDGTIPNAWGSINIDDEGMPTLKKVLIEKGILKGYMIDKLNGRRMGMDSTGSSRRQ
                     SYKFAPTSRMTNTYIATGNDENENIIASTPEGLYAKKMGGGSVNPVTGEFNFAVAEGY
                     MIKNGKIDKPVRGATLIGKGADVLLKIDMVGKDMEMGQGMCGSISGSVPTNVGQPMIR
                     VSELTVGGR"
     gene            8600..9970
                     /locus_tag="PK733_05245"
     CDS             8600..9970
                     /locus_tag="PK733_05245"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOJ09981.1"
                     /translation="MNIMEFDEFKDKLFAAAQKAGFSDYEIYYATGESFRVNVYEKEI
                     DNYSVNSTKGLGFRGLFNGSMGYAYTEILDDNAIELLVNNAKANAAIVENVDSNDIDI
                     IFGGSEKYPEIKCFNDELSNVDENEKIKFALEMERMAFDRDKRVKSVEYCAVQSFEGS
                     ISIVNSKGLELHHRGNGIYSILIPVVSDGERKDTAVAFISTRDFNKMKVESIVNEAVE
                     NALAYMGAGTVNTGKYRVILRNDAAADLLETFSVVFSADRVQKGMSLLKDKTGKAIAS
                     ETVTIIDDPLLEGGMASAPFDGEGVACYTKNVVKGGRLVTLLHNLKTARKDGVSSTGN
                     ASRPSYASTIDVSPSNFYIKPGESSLNELYIKMGDGLLITELQGLHSGANSISGDFSL
                     AAKGFEILNSKITRPVEQITVAGNFYKLLENIEETGSDLKFGIPSSGGCFGSPSILIK
                     ELSVGG"
     gene            complement(9971..11329)
                     /locus_tag="PK733_05250"
     CDS             complement(9971..11329)
                     /locus_tag="PK733_05250"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ09982.1"
                     /translation="MKVAKFGGTSLASAEQIKKVCKVVTSDPDRKIVVVSAPGKRYKD
                     DIKVTDLLIACAESYISKNNADEECDAVISRFSEIGKELRVDKSVIVSIAEDIKGRLN
                     LYSGDNKKFIDLMKAAGEDNSAKLVAGYLKSLGIDACYVNPKDAGLILSSEFGNAQVL
                     PESYNNLKSLRDIPGIVIFPGFFGYSAEGDVVTFSRGGSDITGSILAAAVDADLYENF
                     TDVDCVYSVNPTIIPNPHPIHELTYTEMRELSYAGFSVFHEEALVPVYRKGIPVRVKN
                     TNNPDAPGTTILLERTNITRPVIGIASDDRFCCINISKYLMNREIGFGRRILEILEKE
                     NLSYDHTPSGIDHISIVLNQAQLNKETEERVTRKIKNDLGVDDITVMHNLALIMIVGE
                     GMSQTVGLAARATGAFARAGVNIEMINQGLSEISIMFGVRAEDSIKAVKSLYEEFFGE
                     GA"
     gene            11644..13671
                     /gene="uvrB"
                     /locus_tag="PK733_05255"
     CDS             11644..13671
                     /gene="uvrB"
                     /locus_tag="PK733_05255"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOJ09983.1"
                     /translation="MPEFKIVSDYKLHGDQPEAVKKLVRGINMGYKEQTLLGVTGSGK
                     TFTMANVIERVQKPTLVIAHNKTLAAQLCSEFKEFFPNNCVEYFVSYYDYYQPEAYIP
                     STDTYIEKDASINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPEDYTELMISL
                     RPGMQKDRDEVIRKLVEIQYNRNEIDFKRSTFRARGDVLEIFPASSSESAIRVEFFGD
                     EIEKITEVDPLTGEIKGIRSHVAIFPASHYTTTRAKMDKALVTIEKELEERLRELKSH
                     EKLLEAQRLEQRTRYDIEMLNEIGFCQGIENYSRHFSERAPGSPPYTLIDYFPDDFLM
                     IIDESHVSVSQIGGMYNGDRARKESLVGYGFRLPSAFDNRPLQFEEFEKKINQVIYVS
                     ATPRWYERERSSQIVEQIIRPTGLIDPEIIVKPVEGQIDDLMGEISKRTTQDQRVLVT
                     TLTKRMAEDLTDYLKELGFKVKYLHSDIDTLERMEIIRDLRLGVFDVLIGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNIDGKVIMYADNVTESMRKAISET
                     NRRRKIQMDYNKANGITPKSIQKGIRDIIEVTKVAEESEKYGYKNGISSAGKQVNRPE
                     DGNMDETFEKDNLEKIIEALEKEMRAAAKNLEFERAARLRDRIIELKNGVQ"
     gene            13673..16504
                     /gene="uvrA"
                     /locus_tag="PK733_05260"
     CDS             13673..16504
                     /gene="uvrA"
                     /locus_tag="PK733_05260"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ09984.1"
                     /translation="MIRDAIYIKGAREHNLKNIDVEIPRDKFVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDYIEGLSPAISIDQKTTSRNPRSTVGT
                     VTETYDYLRLLYARIGTPHCYICGKEISQQTVDQMSDQLLSMEEGTKIQLLAPVVRGR
                     KGEYSKLIEDTKKGGYVRIRVDGEVFDINDPIIIDKNKKHNIEIVVDRLIIRPDIRKR
                     LAESIETVLRLSGGLLLVDVIGKEEILFSQNFACSDCGISIEELAPRMFSFNTPYGAC
                     PTCTGLGVLLKMDTDLIIPDRSLSLSQGAIAINGWNLENGDGYSRMIFEALSKHYKFS
                     LDKPVEKLPPHILDIILYGTGGEKIKIEYDRDYGSGTYMSAFEGIINISERRYNETQS
                     EGMKQYYEGFMSRNPCPDCKGARLKKESLSVTVGNKNINELTSMPVSDIKEFFVNIEL
                     TERQKLIAHQILKEINARLGFLVDVGLDYLTLSRSAGTLSGGEAQRIRLATQIGSGLM
                     GVLYILDEPSIGLHQRDNDRLLSTLKKLRDLGNTLIVVEHDEDTMYAADYIIDLGPGA
                     GLHGGYIVAAGNIEEIKKSEGSITGQYLSGRKKIEIPEKRRTPNGKWIEVIGARENNL
                     KDINVKIPLGVFTCITGVSGSGKSSLINEILYKKLAGELNRARIKPGVHDTILGLEHL
                     DKVINIDQSPIGRTPRSNPATYTGVFDYIRDVFSSTNEAKMRGYKPGRFSFNVRGGRC
                     EACSGDGIVKIEMHFLPDVYVPCEVCKGKRYNRETLEVKYKGKSIADVLDMTVEDALD
                     FFQNIPKIQRKLQTLYDVGLEYIKVGQPSTTLSGGEAQRIKLASELSKRSTGRTIYIL
                     DEPTTGLHVADVHRLIDVLQRLVDTGNSVVVIEHNLDVIKTADYIIDLGPEGGNKGGY
                     IVAQGIPEEVAEEKSSYTGQFLKKMLERE"
     gene            16620..18944
                     /locus_tag="PK733_05265"
     CDS             16620..18944
                     /locus_tag="PK733_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOJ09985.1"
                     /translation="MMMCSACNKNIAVVFVTKIINGKQTQEGLCLSCAKKQGIQPLNQ
                     IIEQTGMSEEDIDNLNKQVNNMVDDIDIDSMLEDSENENQGDMGNLFFNFFNKAFPQN
                     KESVDESSSDPKKRNRNDDRQAKNTTRTKTQDKKSSKKKKFLDMYGINLTDKAKCGEV
                     DRIVGRGKEIDRVIQILNRRTKNNPVLIGEPGVGKTAVAEGLAVKIANRQVPAKLFNM
                     EVYVLDLTSVVAGTQFRGQFEGRMKGIIEEAKSLGNIILVIDELHNIMGAGDAEGAMN
                     AANILKPALSKGEIQVIGATTLNEYRKHIEKDSALERRFQPVIVNEPSIQDTVEILKG
                     IKDYYEDYHQVKISDEVIEAAANLSERYITDRYLPDKAIDVIDEAGSRANLKNEFLVE
                     LEALKDELKRVQEEKESAVSADSIEDYQKAADLKVRECKLLGSIKDIEEKNKVVELSI
                     DDIAYVIEAWTSIPVQRLTEIEAEKLLNLEERLHKRVIGQNAAVSSLSKAIRRNRAGF
                     RKRKKPSSFIFVGPTGVGKTELVRALAVELFGSEETLIRLDMSEYMEKHSVSKLIGSP
                     PGYVGYDDGGQLTEKVRRKPYSVILMDEIEKAHPDVFNMLLQILEDGRLTDSQGRVVS
                     FENTVVIMTSNAGTTLKSSGIGFGQDQYKNLEGKVKEVLRETFRPEFLNRLDEVIVFT
                     ELGKDELREIVDLMLNEVKHDVEDKDMTIDITDSAKQYILEKGYEPKYGARPLRRTIQ
                     RYIEDELSELYLKGVYKEGSRILVDCIDGEIVFK"
     gene            complement(19067..20938)
                     /locus_tag="PK733_05270"
     CDS             complement(19067..20938)
                     /locus_tag="PK733_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ09986.1"
                     /translation="MRRKIFSFYFILIMIGLSITGFSTFYLSQRFYKTEVEERMINSA
                     SLIRYQVLETVKRGNTADFNEISKTYSVILSSDDNSANLRDNKSKVRVTFINYEGNVL
                     GESEKDYRNMENHLGRKEIQEAIQGKTGKDIRFSDTIETDFLYIALPIKEIKAIVRVS
                     VPLVQIRKIYQLIWNYELSGMIIGIVLTLLLALKFSTSITQPISELIIKSREISFGNY
                     SVRVKAESDDELGQLAGTFNDMASQLEKIVFELKDKNLKMDTILNSMTDGIIAVDKNF
                     RIILINKVACKLFKIESTNGLIGSDFLEVIRNNNINKMLNETLGKRVVLSNEININYS
                     EYKPGGKVGGKILSITTIPFQPLFLKSEDYNQTLTFNEGAVLSIQDITNIKKLEQMRS
                     DFVSNVTHELKTPLTSIRGFVETLRNGAIHDDEVADKFLEIIDIEAERLYNLINDILQ
                     LGEIESAKNNTNNNANNYTNSNIGSQNIKKLIDESISLLKTQADKKGIIIHVDVQDNL
                     LITANKDRIKQMLINLVDNGIKYNKSGGSVSVSSFKDEGNLVIKVADTGIGIPEEHLP
                     RIFERFYRVDKGRSRSMGGTGLGLSIVKHIVNLYDGNIKVHSEPDHGTEFIIQLPLS"
     gene            complement(21018..21749)
                     /locus_tag="PK733_05275"
     CDS             complement(21018..21749)
                     /locus_tag="PK733_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ09987.1"
                     /translation="MAKELIYVLEDEEHIQQLIKYNLEANGYEVVGFQNGESLLKQIE
                     TSLPGLFILDIMLPGIDGLEVCKSIRRDQKLGTIPIIMLTAKSEELDKVLGLELGADD
                     YITKPFSVRELTARVKALLRRVNALVPNGKEEEKEKEENKEDVIRYKDICIYTDRHEV
                     YKSGKLIDLTLKEFDLLKILMLNKGKVLSRDLLLDKVWGFDYYGETRTVDVHIRYLRQ
                     KIEDDDSNPEYIETIRGVGYRFRDK"
     gene            complement(21900..22610)
                     /gene="phoU"
                     /locus_tag="PK733_05280"
     CDS             complement(21900..22610)
                     /gene="phoU"
                     /locus_tag="PK733_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOJ09988.1"
                     /translation="MVTRRSFEMELKELHMELIKMGSLVEESIENTILALKKQDVILA
                     KKIFMNDDVIDDVEQKIERKCLHLIATQQPLAKDLRSIGAALKIITDMERIADHSADI
                     AEITIRMAGEKYIKPLIDIPLMAELARNMVKRALDAYVRQDMELARQVCSDDDDVDNL
                     FSRIKLELINIVKNDPDSVDQVINFLLIAKYLERMADHATNIGEWVVFNVTGEHEHLA
                     KMLHKDDAKRNPFADIEA"
     gene            complement(22749..23519)
                     /gene="pstB"
                     /locus_tag="PK733_05285"
     CDS             complement(22749..23519)
                     /gene="pstB"
                     /locus_tag="PK733_05285"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOJ09989.1"
                     /translation="MNNAVQNNVKISTRNLNLFYGSMQALKQITMNIEERKVTALIGP
                     SGCGKSTFIKTLNRMNDLISGVNIKGEVLLDGLNIYKDYDVADLRKRVGMVFQKPNPF
                     PISIYDNVAYGPRIHGIKDRARLNDIVEKSLRSAALWDEVKDRLKHNALSISGGQQQR
                     LCIARVLAVEPEVVLMDEPTSTLDPVSTLKIEDLIDELKGKYTIVIVTHNMQQAGRIS
                     DKTAFFLQGNVIEFDDTEKVFSRPVDSRTEDYITGRFG"
     gene            complement(23543..>23855)
                     /locus_tag="PK733_05290"
     CDS             complement(23543..>23855)
                     /locus_tag="PK733_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ09990.1"
                     /translation="GATKLFTIRKVVLPCAVPGILTAVILSTGRIVGETAAVFLTAGM
                     GRNIARNIMESGRTLSVHLYILAKEGISFDKSYATATVLIILIAIINMASSRLAARMK
                     K"
BASE COUNT         8097 a         3871 c         4859 g         7028 t
ORIGIN      
        1 ttacagctaa aaagctgtga attgattata actatattgt acaaggaggt gattttaatg
       61 agtatgccta caattcctga tattaaaccc aaaatcgaaa tatcatttga agaaacaatc
      121 aagttattgc ttgcttcaat tgctcttgaa gaactaagtc ttgctcatat tatgaatgct
      181 gaagcagaaa aagtccagga agttataaag cttggaggct gtaataaatt cgatgaatta
      241 ctatgtataa acaaaagtgt tgaaaaaaca ttgaaggatg ttatcaaaaa ggaaatgctc
      301 ctggaattta agttcgagaa tatacttgaa ttaatgggta aaggcttcaa ggactgctaa
      361 ttttcctatt accaaaaagg ccttgttttt gaaaaagcag ggccttttta cagcactttt
      421 aatcaacagt acaaagtaat atatataaaa aaactgcagg tttttaagta gaaaagtaga
      481 atatagcagg tattatatag caactggaaa ctttctcaat gcaatataac agggagcgtg
      541 gtgagttttg aaagaaagag aaataaatgc tagctttatt ggtaaaaaga taatagagaa
      601 tgctgtgaaa ctagatgagt cattagttga tattcttaaa atagaagtta gaagattaaa
      661 acagtcaata aaaagcaata acaaccttga tgaacttcaa aggactaata atttaatcaa
      721 aaatataatc attgcactta caataacaga tgaaagaata caaactggaa ttgatttatg
      781 tatgaataac agtgaaactg agtagatttc ttgcaggacc gtccccatgt ttctgtttca
      841 gatgtttcac acatatttaa caaataaagc ttaacttttc cgaaaataat ccgattatat
      901 atagtagacc ataaattttt attaacttgg agtgattata tggaccttgc aactttaatt
      961 ggaataattt ctggaattat ttgtaccgca tatccaatat ttgatggggg atatccaaaa
     1021 acttttctta atataccgtc attgatgctt gtagtagggg gaggtattgc atctaccctc
     1081 ataagttata gaataaaaga ggtaacaagg atttttcaag ttgtgggtag tgccttttca
     1141 aggaaagaag tcgatcttga gtcaactata aggcttctgg tggaattgtc gcagaaagca
     1201 aggcgtgaag gacttcttgc tttggaatca gaacaggata acatcgagga cagttttatc
     1261 aggcgttctg ttcagttaat tgttgatggg gttgagcctg agatgataaa ggaatcttta
     1321 gacctggagc ttgataacat ggcattaagg catgagaggg ggcaaagttt gtttagaact
     1381 atggctgttt tatttcctgc atggggaatg atagggacat tgataggcct tataaacctg
     1441 ctgaaagccc ttgataagcc tgaaaatatc ggacctgcca tggcacttgc tttggttact
     1501 acattatatg ggtcacttct ggctcatttt atatgtaacc ctatagctaa taaactttcc
     1561 ttaaagagca aggaggaaat tcagcagaaa gaaatgataa tagaaggcat tctttccatt
     1621 caagcaggtg aaaatccaag aattatggag cataagctta aaacatttct gtcttcagaa
     1681 caaagagaat tgtatgaagc taaaaacgag gatctttttc agcaaaaaga caacaagagt
     1741 aatagcagca gcagaatagt tactgaatag atgggggtgt taatgtgtcc agacagagca
     1801 aaagacatag aacagacagc tcatcagatt cggcaggcga ttggatgaat acatatgcag
     1861 atatggtaac tctgctctta accttcttca tattgctgtt ttccatggca acagtagaca
     1921 aacagaaatt cgttgaattg gcaaattcat tgaggagtgc ctttatgcat gaaaatggtg
     1981 gaggcgattt atatgaaagt aatagaggca gtgagcttat taatatatat gatgaaataa
     2041 atgcagttga taaggtagta tctcttgtaa ataatgatta tgaaggcgga gaaactgaca
     2101 ataacgttga tagaataaca gatggaaacg aagttgaata taatacagat gatcttaaag
     2161 cagaaattga gaaggctata gaagaaatgg ggctggaaga atacgttaaa attattgaaa
     2221 gaaaagactt cctgattcta agatttgatt ctgttattct attcgactta agcagtgcag
     2281 acattaaaag ttctgcaaaa acagtattgg agaaacttgg gcaaatgcta aataaaatga
     2341 gcaatgagat tattatacaa ggacatactg ataacctgcc tataaacacc gggcagtttc
     2401 cttcaaattg ggagctgtcg acaagaagag cgaccaatgt agttcatttc tttattaacg
     2461 aatgcagcat agctccttca aggcttactg ctacaggcaa tgcagaatat aagccggtaa
     2521 aacccaacga tactgcagaa aacagacagc aaaacaggag aattgatatt gttgtaagta
     2581 aggattaaca atatgagatt attttatata aggaatacat cattccaatt ctatcaaaaa
     2641 ttatcccgga agacatcgtt accaaattag catagtagta ttaggcaatt tctgagggta
     2701 cactttctta gattaagaaa atgtgccctc tttttcccgt taacacttga cacgaacata
     2761 ggtttgcgga ataatatata tgtagaataa aaactgctat tcagtattca gtattccgta
     2821 tttgatgtca ggtatctgga acatgatgag aagaaagtga atagttagtt atcaatatga
     2881 atcaaaaagg agagggatgg atggaaaaga ataaaagcaa aaacacatat ggcaacgaaa
     2941 gtatttcatc cttgaaaggc cctgatagag ttagattacg cccgggggta atatttggtt
     3001 ccgatggtct tgaagggtgt cagcatgcag tatttgaaat cctttcaaac tcgattgatg
     3061 aggctagaga aggctatgga agcgtaattg aggttacaag atctaaggat aaatcaataa
     3121 tgattaggga ctatggcaga ggaatccctc ttgaatacaa cccaaaagaa gaaagataca
     3181 attgggagtt ggtctattgt gaactctatg caggagggaa atacaaaaac tatcttggag
     3241 aaaattatga attcagcctt ggattaaacg gtcttggcgc atgtgcaact caatatagtt
     3301 cggaatatat ggacgttact gtattcaggg atggatacag atatgatttg cattttgaaa
     3361 agggctgcaa tatcggcggt ttaataaaga aaaaatccga caatagccaa acaggtacaa
     3421 ttcaaaaatg gagacctgat atagatgtat tcactgatat tgatgtgcca ttaagttatt
     3481 tccaggaagt gttgaaaaag caggcagtag taaattcggg cttaaagttt atactatatg
     3541 acgaagaatc cgacttaacc tttgagtttt tttatgaaaa aggtattatt gattatgtta
     3601 atgaaattaa tgcagacaag ggttttacgg gtgttgaatt ctatgaaact actgcaaggg
     3661 gaagggacag ggaagataaa cctgaatata aggttaaaat agaggcagct ttctgtttta
     3721 acaatgagat taatcttctg gaatattacc acaactcaag tttccttgaa tatgggggat
     3781 ctcctgacaa agcagtaaaa gctgcttttg taaacgaaat tgacaagtgc ataaaagcaa
     3841 ggaacaagta taataaggat gagtctaaaa taaccttttc agatatccag gacagtttaa
     3901 tacttgttac aaactcattt tccacaataa ccagttatga aaaccaaaca aaaaaatcca
     3961 taacaaacag gtttatacag gaagcaatga cagatttttt aaagcaacaa ttggaagtat
     4021 attttattga aaataaatta gaaagtgata aaataataga gcaaattctt ttaaataaaa
     4081 gaagccgtga aactgctgaa aaaaccagac tcaatataag gaaaaagctc agtgggaata
     4141 tagatatatc aaatagagta aaaaagtttg ttgattgccg tacaaaggat atcaacagga
     4201 gagaactgta tatagtggag ggtgattctg ccctgggttc atgtaagcta ggcagagatg
     4261 cggaatttca ggcaattata cctgtaaggg gtaaaatact caactgcctt aaaaccggat
     4321 atgaggggat attcaaaaat gatataatta ccgaccttct taaggtttta ggctgtggtg
     4381 tggaaattaa gtcaaagcac aataaggaac ttaatacatt tgatatggga aaccttaagt
     4441 gggataaaat tataatttgc acagatgcag atgttgatgg tttccatata agaacacaag
     4501 tactggccat gctttatagg cttgttccca cccttataca ggaagggaag gtttatatag
     4561 cagaaactcc tttgtttgaa attaatgcaa aagataagac tttttttgcc tatgatgaga
     4621 aagagaaaat caatataata tcaggtataa agggtaaata cacaatacaa cgttcaaaag
     4681 gattaggtga gaatgatcct gatatgatgt gggaaactac catgaatcct gagaccagga
     4741 gattagtaaa agttgtgccg gaagacgaag aaaaaacccg ggaggttttt gatcttcttt
     4801 taggtgacaa tcttcagggc aggaaagatt ttatagaaga gtacggacat atgtacatgg
     4861 atatgattga tgttagctga tttaagagat aatatgacat gcaaaagctt actatagaaa
     4921 gtttattaga gatagacaat aggtatggaa ggagaaaaag cgtgtttacc cggctttaag
     4981 aagcagggat acttacgcta aatgcttgat gaactcaaat aatttaattg aacaacatat
     5041 atccaatacc cttgaagaaa actttatgcc atatgccatg agtgttatag tttccagggc
     5101 tataccggag attgacggct ttaagccatc acaccggaag cttttataca caatgttttc
     5161 aatgggattg cttacaggta acaggacaaa atctgcaaat gtagtggggc agaccatgaa
     5221 acttaatcca cacggagata tggctattta tgaaactctt gtgaggcttt ctaagggata
     5281 tggtgcgcta atacatcctt ttgtggactc aaaaggaaac ttcgggaaac aatattccag
     5341 agatatgaga tttgcagctc cccgttatac cgaggttaaa ctggataaaa tcagccagga
     5401 aatatttaga gaactggaaa aaaacgctgt agattttgtt gataattacg atggaacact
     5461 aaaggaacca gtgttgctgc ctacaacctt tccaaatatt cttgtaaatc ctaatcaggg
     5521 aatagctgtt ggaatggcaa gtaatatttg cagtttcaat cttcaggaga tttgcgaagc
     5581 aacagtacaa tatttaaata atgagaatac agatatactc aagtacatta aagctcccga
     5641 ttttgcaaca ggaggtcgat taatttacaa tgagagggat attagggaaa tttatgaaac
     5701 aggaagagga ggctttaagg taaggggcaa atataggtat gataagaaaa acagttgcat
     5761 agaaatattt gaaatacctt atacaactac tacagaagcc ataatggatg ctgttattca
     5821 attggtgaag tccggaaaaa ttaaagacat aaccgatata agagatgaaa cggatcttga
     5881 tggtctcagg ctaactatag acataaagaa gaatacaaat gcagatgacc ttatgaacaa
     5941 gttatacaag cttacgcctc ttgaagacac attttcctgc aacttcaata tattgataaa
     6001 tggggtcccc agggttatgg gaattaagac aattctcact gagtggatag catatagaat
     6061 aaactgcata aaaaggctta ccttatttga tattgaaaga aaatcagaaa agctccatct
     6121 tcttctgggt ttgcagaaaa tacttctgga tattgacaaa gctatcagaa taatcaggaa
     6181 cacagagcag gattctcagg ttatacccaa ccttatgaaa gcatttgata tagataaagt
     6241 tcaagctgaa tttgtagctg aaatcaaact tagaaacctg aacaaagaat atatattaaa
     6301 ccgtgtagga gagattgagc agttacaaaa agaaataggg gaattaaagg atatttatgg
     6361 cagcgaaaat aagataaaaa aaataatcat aaaacagtta actgaagtta caaagaaata
     6421 cgggcagcct agaaaaactg agattattca tgaagaattt gttgaggaaa taactgagga
     6481 gcattttata gaagactata atgtaaaaat attcttgaca gagcaaagct atataaaaaa
     6541 gatatctttg gtttcactaa gggctaatcc tgagcaaaag cttaaggatg atgatcatat
     6601 tatacaggaa atagatgccc gcaataaaga tgatttattg ctcttttcag acaaaaccaa
     6661 tgtttacaag ctgaaagtgc atgaattgga agacctcaag gcaagcgtcc tgggagaata
     6721 cctgacaaat aaacttgaac ttgatgaaga tgaaaaaatc atatatattg tagctacagg
     6781 gaattataag gggtacatgc tgttttcatt tgaaaacgga aaaactgcta aaattgatat
     6841 gaatagctat gctacaaaaa ctaaccgcag aaaattgcta aatgcatatt caggcctttc
     6901 aaagcttatt catataatgt atctggaaaa tgaccttgaa ctggttgcat acagcagtat
     6961 tgacaaagtg cttattttta atacagcctg tataaacccc aaaacaacca gagattctca
     7021 aggcgtacag gtactcaagg aaaagaaagg gagctggatg aaggaaataa aaactgttga
     7081 acaagctgat ttatcagacc ctgagtatta caggacaaag aacatcccag ctgtgggctg
     7141 ttatttgaaa aaagaagaca aattaagcga acagcttgaa ttaaagctag agtagcaaaa
     7201 agggagtggt gtaatgattc caaaagatat tattgaagaa gttctatccc gcgctttatc
     7261 actgggtggc gattttgccg aggtttttat cgaggatagg gtaaacaaca gtctcagaat
     7321 ggtgaatgga aagatggagg atgttgtatc aggcagggat tacggtgctg gaataagagt
     7381 tatgaaagac ttcagaagtg tatatgccta tacaaatgac acatcaaggg ataatctact
     7441 tgatacggcc tataaggctg catgtgcttt gaagtatact aagcaggata taactgtaaa
     7501 tctggtagag cgggtttctg ccaatattca cccggtttta tttgtacctt catccataac
     7561 caatatgaaa aaagcctcta ttgttaaaac tgcctataaa gttgcgaaag actatagcca
     7621 tgagattatt caggtagctg caggttatgg agatgtggac caaaagatat taattgcaaa
     7681 taatgaaggc ttatttatac aggataggag agttaggacc aggctcttta tacaatccgt
     7741 ggcaggcgat ggcattgaaa atcagacagg atttgaaggc cctggaaaaa gtatgggatt
     7801 tgaactgttt gatgttattg atgtggaagg ctatgcaaaa gaagcaagca gaattgcagt
     7861 aaccatgctc catgcggaac cctgtcctgc aggaagaatg cccgtagtaa tagataatgg
     7921 ttttggtggt gttatattcc atgaagcatg tggtcatgca ttggaagcaa cttcagttgc
     7981 aaaagatcac tcggtattta cgggcaaatt aggcatgaag attgcttcgg ataaagttac
     8041 ggcaattgat gatggcacaa tcccaaatgc ctggggatca atcaatattg atgatgaagg
     8101 tatgccaacg ctaaaaaagg tattaattga aaaaggaata cttaaagggt acatgataga
     8161 taagctcaat ggaagaagaa tggggatgga ctctacaggg agttcaagac gccagtctta
     8221 taaatttgca cccacatcaa ggatgaccaa tacttacatt gcaactggaa atgatgaaaa
     8281 tgaaaatatt attgcctcta ctcctgaagg tttatatgca aagaaaatgg gaggcggttc
     8341 tgtaaaccct gttacaggag agtttaactt tgcagtagct gaaggttata tgatcaaaaa
     8401 tggtaaaata gataaacctg taaggggtgc taccttaata ggaaaaggtg cggatgtact
     8461 gttgaagatt gatatggtag ggaaggatat ggagatgggc cagggtatgt gcggctctat
     8521 aagcggaagt gtacctacaa atgtagggca gcctatgata agagtatcgg aacttacagt
     8581 tggagggagg taaaagtaca tgaatattat ggaatttgat gaatttaagg acaaattatt
     8641 tgcagctgcc caaaaggcag gttttagcga ttatgaaata tattatgcaa ccggggaaag
     8701 cttcagagta aatgtatatg aaaaagaaat tgataattac agtgttaata gcacaaaagg
     8761 cctgggtttc aggggacttt tcaatggaag catgggatat gcctatacgg aaattcttga
     8821 tgataatgcc atagaactgc tggtaaataa tgcaaaagct aatgcggcca tagttgaaaa
     8881 tgttgacagt aatgatattg atataatatt tggcgggtcg gaaaaatatc ctgaaataaa
     8941 atgtttcaat gatgagcttt caaatgttga tgaaaatgaa aaaataaaat tcgctcttga
     9001 gatggaaagg atggcttttg atagggataa aagggtaaaa agtgttgaat attgtgctgt
     9061 gcagtctttt gaaggaagca tcagcattgt aaattcaaag ggacttgaat tacaccacag
     9121 aggtaatggg atttactcaa ttttaattcc tgtagtgagc gatggtgaaa gaaaagatac
     9181 agccgtagca tttatttcaa caagagattt taataaaatg aaagttgaaa gtattgtaaa
     9241 tgaggcagta gaaaatgcgc tggcatatat gggtgctggg actgtaaata caggaaagta
     9301 cagagtaatc ctgagaaatg atgctgcagc tgatcttctg gaaacttttt cagtagtgtt
     9361 tagtgcggac agagtgcaaa aaggtatgtc actcttaaag gataaaaccg gaaaggcaat
     9421 tgcatcagaa accgtaacca ttattgatga tcctcttctt gaaggcggaa tggcttcagc
     9481 tccttttgat ggtgaaggag tagcctgcta tacaaagaat gttgtaaaag gcgggcgttt
     9541 ggttactctg ctgcataatt tgaaaacagc tcggaaagac ggggtaagta gcactggtaa
     9601 tgcctcaagg ccttcttatg catcgacaat agatgtttca ccttcaaact tttatataaa
     9661 acctggggaa tccagtctga atgaattata tataaaaatg ggagatggtt tgctaataac
     9721 tgaactacag ggacttcact ccggagccaa ctctatatca ggagatttct cccttgctgc
     9781 caaaggcttt gaaattttga acagcaaaat aaccagacct gtagaacaaa taacagtagc
     9841 gggcaatttt tataaattac ttgaaaacat tgaggaaacc ggaagtgatt tgaagtttgg
     9901 cattccttcc agcgggggat gcttcggaag cccgtcaatt ttgataaagg aactgtcggt
     9961 agggggctaa ttaagccccc tctccaaaaa actcctcata taaggatttt acagctttta
    10021 tactatcttc cgcccttact ccgaacatga tacttatctc agaaaggccc tgattaatca
    10081 tttctatatt gactccagcc cgtgcaaaag ctcctgttgc cctggcagcc agaccaactg
    10141 tttgactcat gccttctccg acaatcatga taagggcgag attatgcata acagtaatat
    10201 cgtctacacc aaggtcattt tttatttttc tggttaccct ttcctcagtt tccttattta
    10261 gctgcgcttg attaagaaca atagaaatat ggtcaatacc ggacggagta tgatcatacg
    10321 atagattctc tttttcaagt atctcaagaa tccttcttcc aaaaccgatt tcacggttca
    10381 taaggtattt acttatgtta atgcagcaga acctgtcgtc actggcgatg cctattaccg
    10441 gtcttgtaat attggtacgt tcgagcaaaa ttgtagttcc gggtgcatca ggattatttg
    10501 tattttttac tcttaccggg attcctttac gataaaccgg aaccagggcc tcttcatgga
    10561 aaacactgaa tcctgcatat gagagctccc tcatttctgt gtatgtaagc tcatgtatgg
    10621 gatgaggatt tggtattatt gttggattaa cagaatacac acagtctaca tcagtgaaat
    10681 tttcgtaaag atctgcatca acagctgctg ccagaattga acctgtaatg tccgaaccac
    10741 cccttgaaaa agttacaaca tcaccttcag ccgagtaacc aaaaaatccg gggaaaataa
    10801 caattcccgg gatatctctc aaagatttaa ggttattata agattccgga agaacctggg
    10861 cattaccaaa ttcactgctc aaaataagcc ctgcatcttt aggattaacg taacaagcat
    10921 caatgccaag actttttaaa tagcctgcta caagctttgc actattatct tcaccggcag
    10981 ctttcatcaa atcaatgaat tttttattgt ctcctgaata aaggtttaag cggcctttaa
    11041 tatcttctgc aattgaaaca attacacttt tatctactct cagttccttg ccaatttctg
    11101 aaaaacggga aataaccgca tcgcattctt cgtcggcatt gttttttgaa atatagcttt
    11161 cagcacatgc tattaagagg tcagtgacct ttatatcatc tttatatctt ttaccaggtg
    11221 cagaaacaac aacgattttt ctatcagggt ctgaagtaac aaccttgcaa acttttttta
    11281 tctgctctgc acttgctaaa gaggttccgc caaattttgc aactttcatt tatatatcct
    11341 ccaattccgg tattaaattt atttcttaat atattatatc tatgttaaat ggatgatgct
    11401 attattcata agcctatttt aaaaaaggtt attattaaga ataatacaat ttgtatctgt
    11461 aggtgtcaat atgtttacta aagttttctc tgattattgc ataatttata aagtaaaatt
    11521 gcagaatctc aggcagagca atggggacag tcctgcacat tcacatctat ttagaacata
    11581 cgtttgactt tttcagaatt cgtgatatga tataataaag atagatttat ttggaggtca
    11641 gttatgccgg agtttaagat tgtttcagat tacaagctcc atggagacca gcctgaagca
    11701 gtgaaaaagc tggtacgtgg aattaatatg ggatataagg agcagacctt gctgggagtg
    11761 accggttcag gaaaaacctt tacgatggcc aatgtcattg agagggtcca aaaaccaacc
    11821 ctggtaattg ctcataataa aactcttgct gctcagctat gcagtgagtt taaggagttc
    11881 tttccaaaca attgtgttga gtactttgtg agttattatg attattatca gcccgaagca
    11941 tatattcctt caacagatac ttatattgag aaggacgctt cgataaatga tgaaattgat
    12001 aagcttaggc attctgcaac agctgcttta tttgaaagac gggatgtaat tattgtagcc
    12061 agtgtttcat gtatatatgg ccttggagac ccggaggact atactgagct gatgatctca
    12121 ttaagacccg gaatgcaaaa agacagggac gaagtcataa gaaaacttgt agagattcaa
    12181 tataatagaa atgaaataga ttttaaacgg agtactttca gagccagagg ggatgtactt
    12241 gagatatttc ctgcttcatc ttccgagagt gctatccgtg tagaattttt cggtgatgaa
    12301 atagaaaaaa taacggaggt tgatcctctg actggtgaga taaagggcat caggtctcat
    12361 gtagccattt ttcctgcttc acattatact acaacccgtg ctaaaatgga taaggctctt
    12421 gttacaatag aaaaggaatt ggaagaacgg ctaagggaac taaaaagtca tgaaaagctg
    12481 cttgaagctc aaaggcttga gcaaagaaca aggtacgata ttgagatgtt aaatgagatt
    12541 gggttttgtc agggtattga gaattactcc agacatttca gcgagcgggc tccgggaagt
    12601 cctccttata cattgataga ttatttcccg gatgactttc ttatgattat tgatgaatct
    12661 catgttagtg tgtctcaaat aggcggtatg tataatggtg acagggcaag gaaagaatct
    12721 ttggtaggat atggtttcag actaccttct gcatttgata acagaccttt gcaatttgaa
    12781 gagtttgaaa aaaagataaa ccaggtaata tatgtaagtg caacaccgag atggtacgag
    12841 agagagcgtt cgtcacaaat agttgaacag ataatacgcc caaccggact aatagaccct
    12901 gaaataattg taaagcctgt tgaagggcag atagatgacc ttatgggtga aataagcaaa
    12961 aggacaacac aagaccaacg cgtcctggta actacactga caaaaagaat ggctgaggat
    13021 ttaaccgact atcttaagga acttggattt aaagttaagt accttcattc tgatatagat
    13081 actcttgaac gtatggaaat tataagggat ttacggttgg gagtttttga tgttcttatt
    13141 ggaataaacc ttttaagaga gggacttgat ttacctgaag tatctcttgt agccatactg
    13201 gatgcggaca aagaaggttt cctcagatcg gagacatctc tgatacagac tataggaaga
    13261 gctgctagaa acattgatgg caaagtaatt atgtatgctg acaatgttac ggagtctatg
    13321 agaaaagcta tcagtgaaac gaaccgcaga aggaaaatac agatggatta caacaaagcc
    13381 aacggtatta cgccaaaaag catacaaaaa ggtatccgtg atattattga agtgactaaa
    13441 gtggctgaag aaagtgaaaa gtatggttac aaaaatggga ttagtagtgc tggaaaacag
    13501 gtaaatagac cggaagatgg gaatatggat gaaacctttg aaaaggataa tcttgaaaag
    13561 ataattgaag ctttggaaaa agaaatgaga gctgcagcaa aaaatctgga attcgaaaga
    13621 gctgcaaggt taagagacag aattatcgag ctaaaaaacg gggtgcaata agatgataag
    13681 agatgctatt tatataaaag gagcaaggga acataattta aaaaacattg atgtagagat
    13741 accaagggac aagtttgttg ttattacggg attaagtggt tcaggcaaat catctctggc
    13801 gtttgatacc atatatgcgg aaggacagag aaggtatgtt gagtctcttt ctgcttatgc
    13861 cagacagttt ctgggacaga tggaaaaacc cgatgtagat tatatagagg gtttgtctcc
    13921 ggctatttct attgatcaga aaaccaccag cagaaatcct cgctcaactg taggcacagt
    13981 aactgaaaca tatgattacc taagactgtt atatgccaga ataggaactc cacactgtta
    14041 tatctgcgga aaagaaatat cccagcagac agtagatcag atgtcagacc aattgttgtc
    14101 aatggaggaa ggcacaaaaa tccagctctt agctcctgtt gtaaggggaa gaaaaggaga
    14161 atacagcaag ctgatcgagg acactaaaaa aggtggttat gtgagaataa gggtagacgg
    14221 tgaagtattt gatataaatg atcccattat tattgataaa aataaaaagc ataatattga
    14281 aattgtcgtg gacaggctta taattagacc tgatatacgt aagaggcttg ctgagtcaat
    14341 agaaacagta cttcggttaa gcggaggact tctattggtt gatgttatag gtaaagaaga
    14401 aatattattc agccagaact ttgcctgcag tgactgtgga ataagcattg aggagcttgc
    14461 tccaagaatg ttttctttta atactcctta cggagcatgt ccgacatgta caggccttgg
    14521 agtgctgttg aaaatggata ccgatctaat tattcccgat cggtcattat ccctttctca
    14581 gggggcaata gctataaacg gctggaatct tgaaaatggt gatggatatt cacgaatgat
    14641 tttcgaagct ctttcaaaac attacaaatt cagtcttgat aaaccagttg aaaagcttcc
    14701 ccctcatatt cttgacataa tactatatgg taccggcgga gaaaaaatca agatagaata
    14761 tgatagagat tatggaagcg gtacctatat gtctgctttt gagggaataa taaacatctc
    14821 tgaaaggcgg tacaatgaaa ctcaatctga aggaatgaaa caatattatg aaggatttat
    14881 gagcagaaat ccatgcccgg attgcaaagg tgccagactt aaaaaagaaa gcttgtcggt
    14941 aacggtaggt aataaaaata taaacgaact taccagtatg ccggtttcag atataaagga
    15001 attttttgtg aatattgagc ttacggaaag acaaaagtta attgctcatc agatacttaa
    15061 agaaattaat gcaagacttg gttttctggt tgatgtagga ttggattatt tgacactttc
    15121 cagatctgca ggcacacttt caggcggtga ggcacagagg ataagacttg ccacacaaat
    15181 cggttcggga cttatgggag tgttgtatat acttgatgaa cccagtatag gtcttcacca
    15241 gagagataat gacagactct taagtacttt aaaaaaacta agagatctgg gaaacactct
    15301 gatagttgta gaacacgatg aagataccat gtatgccgct gattatataa ttgaccttgg
    15361 accgggtgcg ggtctccatg gcggatatat agtagctgct ggaaatattg aggaaattaa
    15421 aaagtctgaa ggatccatta caggacaata tctaagcgga agaaaaaaaa tagagatacc
    15481 tgagaaaaga aggacgccaa atggcaagtg gatagaagtt attggtgcac gggaaaacaa
    15541 ccttaaagat attaatgtga aaatcccact gggtgttttt acctgtatta ccggtgtttc
    15601 cggctcagga aaaagttcac tgatcaacga aatcctttat aaaaagctgg ccggagagct
    15661 taacagagct agaataaaac ccggggtgca tgataccata cttggattgg aacatcttga
    15721 caaagttata aatatcgacc aatctccaat aggaaggaca ccaaggtcca accctgctac
    15781 ctataccgga gtctttgatt atataagaga tgttttctct tctacaaatg aagccaaaat
    15841 gaggggttac aagccgggaa gattcagctt taatgtcaga ggaggaagat gtgaagcctg
    15901 cagcggtgac ggtatagtta aaatagaaat gcattttctt ccggatgtgt atgttccttg
    15961 tgaagtttgt aaaggtaaaa ggtacaatag agaaacattg gaagtgaaat ataaagggaa
    16021 gagtattgca gatgtacttg atatgactgt agaggatgcg ttggactttt ttcagaacat
    16081 tcccaaaatc cagagaaagc ttcaaacatt atatgatgta ggattggaat acattaaagt
    16141 aggacagcct tcaacaacac tttcaggagg agaagcgcag aggatcaagc tggcttcgga
    16201 gctgtctaaa agaagtaccg gaagaacaat atatatactt gatgagccga caacaggcct
    16261 tcatgtagca gatgtgcaca ggttgattga tgttctgcaa agattggttg atacagggaa
    16321 ttcggtggta gtaatagaac ataaccttga tgttattaag actgctgact atattattga
    16381 cctaggtccg gaagggggaa ataaaggtgg ttatatagtt gcacagggta taccggagga
    16441 agtcgcagaa gaaaaaagct catataccgg gcagtttctt aagaaaatgc ttgaaagaga
    16501 gtaaaattat tttgacagaa aaagataaaa aaagcatatt aatacaatag accgttttgt
    16561 agatatatgc tataattata agataggtgt aaattagaag ttggattggg gatgataata
    16621 tgatgatgtg ttctgcttgt aataaaaaca ttgcagtagt gtttgtaaca aaaattatta
    16681 atggtaaaca aactcaggaa gggctttgcc tttcatgtgc gaaaaagcag ggaatccagc
    16741 ctttaaatca gataatagag cagacaggca tgtctgaaga agatattgat aacttaaata
    16801 aacaagtaaa caacatggta gatgatatag atattgatag tatgcttgaa gatagtgaaa
    16861 atgaaaatca gggagatatg gggaatttgt tttttaattt ctttaacaaa gcttttcccc
    16921 aaaataaaga aagtgttgat gaatcttctt cagaccctaa aaaaagaaac agaaatgatg
    16981 ataggcaagc aaaaaacact acccggacaa aaacccagga taaaaagagc agtaaaaaga
    17041 aaaagtttct ggatatgtat ggtataaatc ttactgataa ggctaaatgc ggtgaagtag
    17101 atagaattgt aggcagggga aaagagatag atagggtaat acagatactg aacagaagaa
    17161 caaaaaacaa tcctgtcctc ataggcgagc caggagtggg caaaacagca gtagcagagg
    17221 gcttggcagt taaaatagct aacaggcagg taccggccaa gctttttaat atggaagttt
    17281 atgttttaga tctaacatcg gttgtggcag gtacccaatt tagaggtcag tttgaaggac
    17341 gaatgaaggg cattatagaa gaagcgaaat ccctcggtaa cattattctt gttatagatg
    17401 agcttcataa tattatggga gcaggagatg ccgaaggagc aatgaatgcg gcaaatatcc
    17461 ttaaaccggc cttgtccaaa ggtgaaattc aggttatcgg tgctactact ctgaacgaat
    17521 acaggaagca tattgaaaag gattctgctt tggagagacg tttccagcct gtgatcgtaa
    17581 acgaaccttc catacaggat acagtagaaa ttcttaaagg aataaaagac tactacgaag
    17641 actatcacca ggtaaaaata tccgatgaag taatagaagc tgctgccaac ttatctgaaa
    17701 gatatattac tgatagatat cttcctgata aagctataga tgttattgat gaagcaggat
    17761 ccagggcaaa tttaaaaaat gagtttctgg ttgaacttga agctttaaag gacgaattaa
    17821 agagagttca ggaagaaaag gagagtgcag tgtctgcaga ttccattgag gattatcaga
    17881 aggctgctga tttgaaagta agagaatgca agcttttagg aagcattaag gatattgaag
    17941 agaaaaacaa ggttgttgag ttgtcaattg atgatattgc atatgttatt gaggcctgga
    18001 ccagtatacc tgttcagagg ctgactgaaa tagaagcaga aaagctctta aatcttgaag
    18061 aaaggcttca caaaagggta ataggacaaa atgcagctgt aagcagtctt tctaaagcaa
    18121 tacgcagaaa cagggcaggc ttcagaaaaa gaaaaaaacc aagttccttt atatttgtag
    18181 ggccgacagg ggtaggtaaa acagaactgg taagagctct tgccgttgaa ctgtttggca
    18241 gtgaagaaac ccttataagg ctggatatgt ccgagtatat ggaaaaacat tctgtctcca
    18301 agctaattgg ttcaccccca ggttatgttg ggtatgatga tggagggcag cttaccgaaa
    18361 aggtaagaag gaagccatat tcagtaatac tgatggatga aattgaaaaa gctcatcccg
    18421 atgtgttcaa tatgctgctg cagattcttg aagatggaag actgactgac agccagggca
    18481 gagttgtaag ctttgaaaat acagtggtaa ttatgacttc aaatgcaggc acaactttga
    18541 aatcaagcgg cataggattt ggtcaagatc agtataagaa tctggaaggt aaagttaaag
    18601 aagttttgag agaaaccttc agacctgaat ttctgaacag gttagatgaa gtaattgtct
    18661 ttactgaact tggtaaagat gaactgagag agattgtgga tcttatgctg aatgaagtaa
    18721 aacatgatgt tgaagataaa gatatgacca tagacattac agattcagca aaacaatata
    18781 tccttgaaaa gggatacgaa cccaaatatg gtgcaaggcc attaagaaga acaattcaaa
    18841 gatatattga agatgaattg tcagagcttt atttgaaagg tgtgtacaag gaaggaagca
    18901 ggattcttgt tgattgtata gacggagaga tagtttttaa ataaatgatt caggaactat
    18961 atagaagcag gttttaaaac atttaaaacc tgcttctatt acctttgttg atggtgctaa
    19021 aaaattatct cttattatct cctattattt tgatgctgct tgaatgtcag ctcagaggaa
    19081 gttgaattat aaactctgtc ccatggtcag gctcgctgtg gactttaatg tttccgtcat
    19141 aaaggttgac gatgtgtttt actatagaaa gccccaggcc agtacccccc atgcttcttg
    19201 accttccttt gtctacccgg taaaatcgtt caaaaatcct gggaagatgt tcttccggta
    19261 tgcctatacc cgtatctgca acttttatta ctaaattgcc ctcatcttta aatgatgaaa
    19321 cagatacact tcctccagat ttattatatt ttataccatt atcaaccaag ttaataagca
    19381 tctgttttat ccgatcctta ttagctgtaa ttaaaagatt atcctggaca tccacatgaa
    19441 taatgatacc ttttttatct gcctgcgttt ttagtaacga tatggattca tcaattaatt
    19501 tcttaatatt ttgacttcct atattactat tagtgtaatt attagcgttg ttattagtat
    19561 tattttttgc tgattcaatt tctccaagtt ggagaatatc atttataagg ttatataatc
    19621 tttcagcttc aatatcaatt atttcaagaa atttatcagc tacttcatca tcatgaatag
    19681 ctccattcct gagggtttcc acaaaaccac gaattgatgt gagcggtgtc tttaattcat
    19741 gtgtaacatt tgatacaaaa tccgagcgca tttgttcaag ctttttaata ttagttatat
    19801 cctgtatgga taaaactgca ccttcgttga aagtaagtgt ttggttataa tcttctgact
    19861 tgagaaatag aggctgaaaa ggtattgtag tgatgcttag aatttttccc cctacttttc
    19921 cccctggttt atactctgag tagtttatgt tgatttcatt tgataaaacc actctttttc
    19981 ccagagtttc attaagcatt ttattaatat tattgttccg gataacctct aaaaaatcgg
    20041 aaccgatcaa tccgttggtt gattctattt tgaagagctt gcatgcaact ttatttatta
    20101 atataatcct aaaattttta tctacagcta tgatcccatc agtcatactg tttaatattg
    20161 tatccatttt caggttttta tcttttaact caaacactat tttttccagc tgcgaagcca
    20221 tatcattaaa tgttcccgca agctggccaa gctcatcatc tgattcagct ttaactctta
    20281 cagaatagtt gccaaaagat atttcacgag attttataat aagttcgcta ataggttgtg
    20341 taatagatgt tgaaaactta agagcaagca gcaaagtgag gactatacct ataatcattc
    20401 ctgataattc ataattccag attagctggt atattttccg tatttgcaca agtggtactg
    20461 aaacccttac tattgctttt atttctttta taggtaatgc aatatataaa aagtccgttt
    20521 caattgtgtc actgaaacga atatccttcc cagttttacc ctgaatagct tcctgaattt
    20581 ccttcctgcc caggtggttc tccatgttcc tgtaatcttt ttctgattct ccaagaacat
    20641 taccttcata atttataaat gtaactctta ctttgctttt gttatcacgg aggttagcag
    20701 aattatcatc agaagataat attacagaat aggtttttga aatttcatta aagtcagcag
    20761 tattgcctct tttcactgtt tccaatactt gatatcgtat taatgatgct gagttaatca
    20821 ttctttcttc aacttctgtt ttatagaagc gctgtgaaag atagaatgtg gaaaaacctg
    20881 ttattgataa acctatcatt attaatataa aatagaagga aaaaattttt cttctcatag
    20941 cagctccctt catgacattg aatgagttat tgggccaaaa aatcaaacta tttatttcaa
    21001 tattatttat ctctgaatta tttatctctg aaacgataac ctacacccct tatagtttct
    21061 atatattcag gattgctgtc gtcgtcttct attttttgcc ttaaatatct tatatgaaca
    21121 tcgactgtac gagtttcacc gtaatagtcg aatccccata ctttgtcaag cagtagatcc
    21181 cttgacaata cctttccttt atttaacatg agaattttca ataggtcaaa ttccttcagg
    21241 gtcaaatcaa taagcttgcc actcttatat acctcatgac ggtcagtata aatacatata
    21301 tctttgtatc taattacatc ctctttgttt tcttccttct ctttttcttc ttcttttcca
    21361 ttaggcacaa gagcattgac tcttcgcaat aatgccttta cccttgctgt aagctccctt
    21421 acactgaaag gcttggtaat atagtcgtct gctcccagtt ccaatcctaa aaccttatcc
    21481 aactcttcac tttttgcagt aagcataatt atgggaatgg ttcctagctt ttgatcacgt
    21541 cttatacttt tgcatacctc aaggccatca atacccggca gcattatatc cagtataaaa
    21601 aggccgggga gagaagtttc aatttgtttt agaagacttt caccattttg aaaacccaca
    21661 acttcataac catttgcttc aagattatat ttaattaact gttgaatatg ctcctcatct
    21721 tccagaacat aaattaattc ttttgccatg ataaactcct tcaatagtct aaaatcataa
    21781 taaactaaaa tcataatcac aatataattt taccacattt attggggaca tttctcaaca
    21841 taaaatagat actacctgcc agggagatac atctcttgac atataaagcc acaggatatt
    21901 caggcctcaa tatctgcaaa gggattacgt ttcgcatcat ctttatgaag catttttgcc
    21961 agatgctcat gttcacctgt tacattgaat acaacccatt cgcctatatt tgttgcatga
    22021 tctgccatcc tttccaggta ttttgctata agtagaaaat taataacctg gtcaacagaa
    22081 tcagggtcat tttttactat atttattaat tcaagcttta tccttgaaaa cagattatct
    22141 acatcatcgt catcagaaca tacctgcctg gcaagttcca tatcctgtct tacataagca
    22201 tccagagctc ttttcaccat gtttcgggct agttctgcca ttaaaggtat gtcaattaaa
    22261 ggctttatat atttttcacc agccattcgg attgttattt ctgctatatc tgctgagtga
    22321 tctgctattc tttccatatc tgttatgatt tttaaagctg caccaattga tcgtaggtcc
    22381 ttggcgaggg gctgctgagt tgctataagg tgcaggcatt tcctttcgat cttctgctca
    22441 acatcatcaa taacatcatc attcataaaa attttttttg ccaatattac atcctgcttt
    22501 tttaaagcca atattgtatt ttctattgac tcttcaacaa gactgcccat ttttatcaat
    22561 tccatgtgca attctttcag ttccatttca aaagaacgtc gtgttaccat ttaacaattc
    22621 cacctttccg tatgcattta ttaccaaatt cttcattaac tctttcaaat tgtaaattat
    22681 atagcctgat tttatactac ctgatgttac cttacctgat atcatatcac atgacgttat
    22741 attaactttt atccgaaccg tccggttata tagtcttctg tcctgctgtc tacgggcctg
    22801 ctgaatactt tttccgtatc atcaaattca ataacattac cctgtagaaa aaatgcggtt
    22861 ttgtcggata tacggcctgc ctgctgcata ttgtgagtta ctattactat tgtatatttt
    22921 ccctttaatt catcaattag gtcttcaatt ttcaatgtag atacagggtc cagggtagat
    22981 gttggctcat ccattaaaac tacttcaggt tctaccgcta aaactcttgc tatacaaagt
    23041 ctttgctgtt gtccccctga tatgctcagg gcattatgct taagacgatc ctttacttca
    23101 tcccatagtg ctgcacttct gagacttttt tcaacgatat catttaacct ggctctgtct
    23161 ttaataccat gtatccttgg tccatatgca acattatcat atatggatat tggaaaagga
    23221 tttggtttct gaaacaccat tccaactctt tttctcaaat ctgctacatc gtaatcctta
    23281 tatatattta aaccatcaag cagtacctct ccttttatat ttacaccgga tataagatcg
    23341 ttcattctgt ttaaagtctt aataaatgtg gactttccac atccggaggg acctattaat
    23401 gcggtaactt ttctctcctc aatattcata gttatttgtt ttaatgcctg catactacca
    23461 taaaataaat ttagattacg cgttgaaatc ttaacattat tctgaacagc attattcatc
    23521 atactaaatc tttcacctca tttcattttt tcattcgggc agcaagtctg gatgatgcca
    23581 tattaattat tgctataaga attatgagca cggttgcagt tgcatatgat ttatcaaatg
    23641 atatcccttc ttttgccaga atatataggt gtactgaaag tgttcttcct gactccataa
    23701 tgtttctcgc tatatttctt cccatacctg ccgttaaaaa tactgcagcg gtttctccca
    23761 caatacgccc tgtactaagt attactgctg taagaatgcc cggaactgca cagggcaata
    23821 caacctttct gattgtaaac agtttggttg caccg
//
LOCUS       DAOSDD010000038        23643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_962_length_23643_cov_5.331736, whole genome shotgun sequence.
ACCESSION   DAOSDD010000038 DAOSDD010000000
VERSION     DAOSDD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23643
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_23643_cov_5.331736"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..385
                     /locus_tag="PK733_05295"
     CDS             56..385
                     /locus_tag="PK733_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ09991.1"
                     /translation="MNTKEHIKNHFMDETICQTSIATEMEVSTSYLSRIFKQYTNQNM
                     VDYINELRVEKAKLLLENEKLTLCEIAKMAGCSDISLIRIFKKHEGVTPGKYRDYVAS
                     KRADMAD"
     gene            513..1301
                     /locus_tag="PK733_05300"
     CDS             513..1301
                     /locus_tag="PK733_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ09992.1"
                     /translation="MSYLNKSDIINGLRKLGLSDGDHVMVHSSLKSFGYVEGGANTVI
                     DAVLETIGSDGTMLLPTNVFQGSVTVFLRDKIRDNKGIDLRVEKSMTGIITETLRNRA
                     NSLRSIHPSHPVAAIGKKAEELLSEHHIGDSPAGKKSPYGKLAELDKGKILLLGVTNS
                     RNTSIHTAEEYYSSYIFIGETFDTKVIALDGKTHHVNVKGYCVNTPRDFTALDDELIN
                     KSIMSRGLIGNSIAALINSKDLLSFMREEIGKNPLILLSHKNNT"
     gene            1552..3153
                     /locus_tag="PK733_05305"
     CDS             1552..3153
                     /locus_tag="PK733_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09993.1"
                     /translation="MKNSFIDLFRQPDDILVQTQDSGIRFEEPKERTVSPTKIEYVYS
                     DKEARVIVHPCKIPLKRVKLRWNNRNFEIERVLNDCFDRCTNNLAWDCMKPDRELPWY
                     FATYDGERTHCYGVKTGCNSLCFWQIDPYGVSLWLDIRNGGNGVELIEPLIAAIVISR
                     EGEEGETAFTACQEFCKIMCDMPKMPKQPVFGVNNWYWAYGNVTYESVMKETAYLAEL
                     ASDAVCKPYMVIDDGWQPLRHNNNWGNYIGGPWNGCNMGFTDMEKTAADISEYGCIPG
                     LWFRALLTAKRLPDEMLLKRPAAVMNCGQVMDPSNPETLHEVFTDARCISDWGFKLIK
                     HDFSTSDITGGIIASDGWNFYDRTKTTAQITKKFYETIQQGANDAIVIGCNTYSHLTA
                     GIHEIHRSGGDTSGRLWEYTRRNGVNSFMRLVQNGTFYNIDPDCAAFTERVSNELNIK
                     FLELCAITGSTTLASITPGILNTEEKRKIREIYKIASMKGNCAEPLDWLNTSCPSRFL
                     YKDNEYFFDWYSGYNGVRMFPTWMA"
     gene            3334..4437
                     /locus_tag="PK733_05310"
     CDS             3334..4437
                     /locus_tag="PK733_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09994.1"
                     /translation="MSFDMLEYNRSDFDEKVFRSAPDVLVEAVSMKGVAPENYHATTI
                     FPEYFKVKGKWILAEKSRMDCVAVLKSNNEKQIDKIEIKEFRKLEPGDKVILGRTEDG
                     SQGIYVHTSGFSSKKDSTDSFAFRTGKSRETSYSRDYDRLYDLLKYERDNGYIVWVLG
                     PAVVFDADSRRAMVALIENGFADAIFAGNAVATHDLEAALFHTALGQDIYTKNYLPNG
                     HYNHIDAINKARRAGSFEKLIQEEKINDGIVYTCLKNKIELILAGSIRDDGPLPSVIS
                     DVYAAQNSMREHARKATTVLMLATQLHSIATGNMTPSYQVINGMVRPVYIYTIDISEF
                     AVNKLRDRGSLEVSSIITNVQDFLVHLERNLAK"
     gene            complement(4537..4950)
                     /locus_tag="PK733_05315"
     CDS             complement(4537..4950)
                     /locus_tag="PK733_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ09995.1"
                     /translation="MRTWLVDIINKLFDEIDNKHMSKNKEIIDKVMEYVSTNVRNEEL
                     SLKSVSDALYISSNHLSRLFKEYTGITFVEFLSNCKIEEGKKLLLTTNLKVEEISSLV
                     GYSTSQYFISRFKLKYGVTPKDFRIKNGISCNLEV"
     gene            complement(5037..6959)
                     /locus_tag="PK733_05320"
     CDS             complement(5037..6959)
                     /locus_tag="PK733_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ09996.1"
                     /translation="MVEKTSSVNHQMLKTFFPRLNNYFPRREKVSSFLANLIISYWLI
                     IVVCICIMGILSYNYIGNNIKKHTIELNKETLIHFRNQIDNFLLEGINDTSLKILQDC
                     RTNSHLSFYFSNPLEGHVVDVNKIKEYVDSIKSVNTISSKIAIYYKYNNLLVSTDCIR
                     HTLYQEPQYINNDELNYYQAIVDSIDFSNNIYWYFEDNYPFKYLLKDETNQSMIHFIR
                     KIPGKVNNSGVIIISIDENVLRKNIKKSAPSSLDNIIIVDYDGTIISHTHSKYIGKNI
                     CDFDFGKNVVTSQGNSGYFLCNASGTQSVVSYTTSAYNSWKYINVTPLAQYNNGIGFL
                     LNTILLTSFLTIFLGLIVSVFSAKKVSSPLVRLVDFCKNVSNIKKPGNSLNTISSIVT
                     DMSDELKLHKLQIEKSYPILKINFIRMLTTNEYLDNQEINDKMELLNISFPYKNFLAV
                     IIILSGTEVNRNYTQLELEYEKLTIVNTIEKQLNMNNSVCLCGQVDDHILAVINTDIK
                     YYELIEILTSQIFANLNNTTKLFRKYIGIGSYVDNIMNVIESYNSASASIEYSYIYPE
                     QTTFRADEKLYKDCSIGIKYKKLVGYLESSLKSKERNKSIKNVDAIINYIKSTSINID
                     YAKKYLQGLFYQLIKI"
     gene            complement(7274..8398)
                     /locus_tag="PK733_05325"
     CDS             complement(7274..8398)
                     /locus_tag="PK733_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HOJ09997.1"
                     /translation="MISTGTDEKTQILTMFGNISIVRDSVFCRRCHEGYGILDKQLEI
                     YRKHRITKGMTELITYLSQLLPSFERASEALKKTVGVEVSPTQMQIVSEEVGEKVFKE
                     DMEKARKAYEKPEEAAPQELPKDREEGRLYALTDGLQVNTRVEDKDGSTWKEMKMGMV
                     FRDRNIIMRENGDHIITKKEYVSYLGSVNEFKKLMFAATARAGYGKVKELVVIGDGAQ
                     WIWNMCEELFPDAVQILDYYHFSENTHAYAKLIYPEDEVGRKKWVNEVLDAVTNGRVD
                     KAVELVEEKKVDKVPDTVVNLYTYIHNNRERIDYKAFKKKGYYIGSGAIESANKMVIQ
                     QRMKQSGMRWSINGGQYIASLRTKYESKLWDKVVDVINAS"
     gene            complement(8421..8642)
                     /locus_tag="PK733_05330"
     CDS             complement(8421..8642)
                     /locus_tag="PK733_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ09998.1"
                     /translation="MIKNTNEILFKNIPEAEGILRELMQEIIRQEADGKVTINTVEQL
                     IGEAMSGFAKIAVGMSGQLLSNIEVEKKR"
     gene            9116..10738
                     /locus_tag="PK733_05335"
     CDS             9116..10738
                     /locus_tag="PK733_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ09999.1"
                     /translation="MFNIKKKSCILLALILALSTIAASCSSKDSGTSKNEGSTQSDSN
                     NSSVTTDKEVKTDPPTKLRFMYSTTGTSVPGDVDPCSNPFLNIIAELANVEFTEVIVP
                     PYADYDGKFKLTVASGDMPDVMHDQWSFNEVTKYGIDGAFEPLNNYIANSSFISKIYS
                     PAQIDLMKAVDGNIYGLKSRASGDLTCLEYRRDLIDELNNGVVPETPEGWFDVAKKLK
                     AKYPESIPFSGHKMPDVWMFSSFGVSCAGWQYTNGEFIHCFEAPLIKECIQFIKKAYD
                     EGLLDKNFVTNQTADYLNVMFNKNLLCDDYTYLNVVGDMANAYDRNLNGLIFAPAPLP
                     MKNDSRIDISNVYSGPGLLGGSSLCVASGSEEKEGAFRLIETFCSDEVLNLASWGREG
                     IEYNIVNDEKVLDLENSTKTDYRGMYGIMKYYNYPEQIKVIKAHVFSYFPEIKDSLNK
                     IYDDAWDILEEKLKLVPAVTPQDFLKLEADTLSRKSEAKERAESIILRAIIGEISMEE
                     YDKQVSEFLKKYQSITNEYNKKADEIKKDWKY"
     gene            10842..11783
                     /locus_tag="PK733_05340"
     CDS             10842..11783
                     /locus_tag="PK733_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10000.1"
                     /translation="MEQLVKKAYVNLNKVKFNPGRDIPIYFLLLPAVVLVIIFNYMPI
                     YGIIIAFQKFSAFKGVFGSQWVGFDNFKYFLLDENFWRVFRNTVIINIYQIIFGFLVP
                     IIFALLLNEITRNKFKKVIQTVSYLPHFISWVVAAGIFNSILSPTNGLVNVILNELFG
                     IKPIYFLAKQEYFRSIIVLSCIWKGFGMSSVYYLASLSAIDTQLYEASYIDGAGKWRQ
                     TWHITIPGLKSIAVILLILQIGSMVSIGFEQIFLMYNPLVYDVGDVISTYTFRLGIEQ
                     TRYSLTAAIGFTQSVINFILLYAANMLSKKIIGWSMW"
     gene            11826..12707
                     /locus_tag="PK733_05345"
     CDS             11826..12707
                     /locus_tag="PK733_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10001.1"
                     /translation="MKRTKGEKIFEIFDMIILLIIISTIILPIMHIISVSISNKEAVL
                     NLSIGIFPKGFTIEAYKDVISRKTFLSSLCNSIYLTILYTFFSLLVNTMCAYAFTKYF
                     YGKKYLNIFFIITMYFSGGLIPTYILITNYLKMHNTFWAMLLPGIVNVFYMIIMRAQI
                     EAIPKELSEAAIVDGAKEYQVLYKVILPAIKPTIAAVGMFFALDMWNMWFSCMLYTNK
                     ESMWTLQYFLRMMVFNKDLNVDRSSKIAISIDRNNIAPANFQMAAIVLVALPIVSIYP
                     FIQKYFVKGILVGSVKG"
     gene            12800..14914
                     /locus_tag="PK733_05350"
     CDS             12800..14914
                     /locus_tag="PK733_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10002.1"
                     /translation="MSLNKNKYLNIIEYDNYINVDNDCLNIKINRKTGSIAEFLLKEK
                     NVDILDGEIGYIALHYKTAEQLCFTPDDVKLKSYLINKSDESNVLYMNLVSCKCDTEL
                     NIELKMEDGNIRLNCTPITSRPKMCEVEVVYVLPFFKANVNGFTPYSDADLLPEEIDH
                     KLFIYGGDQFRGELSDSISLPLAGFYNKNENYGITVVQPVDIYKPQVQYYYVRENAPL
                     SFVVKYCNLRLSKYDSPKTSIIFSVHEGDWRCSLKAIYDLYPEYFNVNNNSIYETEGT
                     MLCSTLQPEENIKNWVLQQDLKWQEIHANIFPYYGLYAPEVEEWNDISNVYDISKLNL
                     NRLIDIEFWEMGHSMMKYSNDKKIAKKDVNNYIEMLHKYGIGAFIYINAVILNKYEKE
                     KYKDSEAIGLDGLPCFKNYYLNNPMNPDPGLSWGQNIIYQIEKILNYFPECDGLFFDE
                     LHFRHFDMNHDDGITAVNDKPSYMIGFAIEKISKLIYEKIENSGKSIWGNGPTSLEVM
                     RYIDGLMAENSWKWLGTVQYYALNKPIVLLQSRTESIIELEEILKNAFICGSQVHVVW
                     KPDSTRGTMETGEMLPDNDKEKLALLAGYSKILKLLKKRSWYLKAHCLNVLDDNYKGN
                     IFKIPSGGYVVTLINKKHASCYESVEIELLLDDDFTVNKIYNVDPESDENTKIEWGKI
                     ANSINGVYIKLKIKNSIAIVIE"
     gene            14955..15725
                     /locus_tag="PK733_05355"
     CDS             14955..15725
                     /locus_tag="PK733_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10003.1"
                     /translation="MQIIDAHQHYYMEPQTIENLIEKSKMADITKVCLSGLGDYSCDK
                     YNEDVRKAIEKYPDTIIGFGFIRLGIDTWHLVDELFEQGFRGLKTINPRKNYSDKEYY
                     PIYKRAEELNMPILFHTGIKIFSPQDVKHDVACERMRPIFLDAVARAFPRLNIIGAHL
                     GVPWYNEACAVSEENPNVYFDLSGAINILYKLEPWFFKILTYWEMAKGKFVFGVDDHY
                     ERIPEAVNKFNILMDKLNFSQELREKIFYGNMEKILGL"
     gene            15740..16750
                     /locus_tag="PK733_05360"
     CDS             15740..16750
                     /locus_tag="PK733_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10004.1"
                     /translation="MVMFTAKEKLKNGEWLTIKAIKSPTAEYEDRIFSYINPKMPWRQ
                     DTYKRINGDIADQCKDMFFIGEIDGEIVSLMWYTVSDDVGTYGMVSTSEKHRNKGICG
                     CIMKYCIQFMDSEPGLQAVYLGVSNPMAYKIYEKNGWRPYNNGTLNDTCIMRRLINCN
                     IDKKEFDDNYYKKYQNDGSRMIIRRVIRGDLPKLEALYNLAESTVMIKDYNASIYIGT
                     AVECQIIDLINKMELGEGLFYCIENVLGRIVGAATLYNKENGYRTGSDNIHINISSNR
                     IGNEISGGISNEIINTSSNELGIRVLDFFVHPNYEEYTKSLVGEVSANINSTLESCYG
                     DL"
     gene            17032..17979
                     /locus_tag="PK733_05365"
     CDS             17032..17979
                     /locus_tag="PK733_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ10005.1"
                     /translation="MSNYIRVSSLGSPGALYAEGETDYQKLVDKMKLMWQEELEKVLP
                     DKPDLIVLPECCDEFSGLSSKENIEYYRVRKDQIRDYFMKTAKENNCYIAYSAMRYIP
                     DGSIRNSTQIIDRDGNIAGIYNKNHPTIRDHEINGTLYGKNANVIKCDFGKVACVICF
                     DLNFDELRLKYVEQKPDLVIFSSYYHGGLMQNYWAYSCRTYFVGCIMGLQNTIISPVG
                     EVVARSTNYFHHLSHNINLDYIVCHLDYNWHKLDAIKKKYGTKVNIYDPGYLGSVLVS
                     SESEDLAAMDLAKEFELELLDEYFERCRNHRSMPGKIEL"
     gene            18090..18806
                     /locus_tag="PK733_05370"
     CDS             18090..18806
                     /locus_tag="PK733_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOJ10006.1"
                     /translation="MKKDIIKVLNKIYSKIFGKIYWKDIGCIIIGTLTVAFCINVFLV
                     PYKIIEGGVTGIAIILHYISGERLPVGLTAFTMTLPLFIAGFYVLGKSAGFSTIIATV
                     LLSLFIDVISPISKAIVYRYFISPDEILNYPVIMLFYCVISGIILGTGYGIIYKTGAS
                     TGGSDLAAMIINKYLPKVTLGIIIIFLDGAVIILSGVVFKSFENTIYAVFRAFISAGV
                     VDYILKCSRKQMKNGVNNSY"
     gene            19013..21418
                     /locus_tag="PK733_05375"
     CDS             19013..21418
                     /locus_tag="PK733_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10007.1"
                     /translation="MISRANKFTKLFYRYLVSYILLFLIPLMLFGTIIYISSFKIFKN
                     EVEDYNKSKLYQTMELLDNQLKNLERIAVNISFNKNLYPYAIEDDDYWTNGDFLELKA
                     YLASNTINNEIIDEIILYLNNKDIIFTSRGKTSIETLFENIIRVNAKDASLLTKKIQC
                     LETPEIIKISKRDKDTSLLVQETYDIFQGNEKSYIFYAYPAYMYPIPQVRGDTYGSVV
                     FRINCSKLNDIMKATLGDMVGEIHIFTNDFNELSSIESDKLLSSKEIKDVLSNSNNRQ
                     ISYFEGKYGQQSCSTMYIDNKISVIDIQSKLTGYHYVAVLKVIDYFNKVAKMQQKILA
                     LLIAVVLAGIVMSTVLAFKNYRPVWSLMKLMNNIRSNKKNNKDSDVLYYFRNEFDEIE
                     TSINNVLEENRLLSDKVSLQRPYVEDQIISMLLKGNIRNKNKAMEILSLYDIEMTNLY
                     FAVLVLSICSNRESVNDISFSELSYCINIFFEQNFSGSFYTLEMAKGNVVVTLNSEYD
                     DLLRDNLQELLTGLQALLYRNIKIKINVGIGRIYGDILDISTSFNEAISSIDYSMIKG
                     PESFVFFEDIINRDDKIYWYPLEEQTRLIQSLKQGNIDMFTDVLAKVCNIIKSKNISA
                     GMIKSICFGIINCIINIVNELETDEFTNELEDMMEFRSLEDLQNKLEKMASSICNYIN
                     EKKECGNEILKAAMLEFIHNNYAENGLCLEMLAEEFNLTAKYLSKIFKDQVGCKFADY
                     VRQLRIKHAKKLLTDTDMSIREIVNAIGYGDTANFIRTFRLFEGVTPGDFRKIKKPST
                     IEY"
     gene            21592..22557
                     /locus_tag="PK733_05380"
     CDS             21592..22557
                     /locus_tag="PK733_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10008.1"
                     /translation="MRATSQASLSKSDNILYRKKNKSFSKILKENYELYLFLLPAFMY
                     FIVFRYWPMYGLQIAFRKFNGAMGVWGSPWVGLKNFRNFFDSYYFWILIKNTIGITLY
                     SILVGFPMPIIFALMLNEVHNDKFKHFIQTVSYAPHFISTVVLVGIIIAFLSPTTGVI
                     NHIIVALGGEARPFMIMAGWFKRIYVWSGVWQNMGWSAIIYIAALSGIDPQQHEAAVI
                     DGANRFERIWHINIPGILPTIIVLFILNMGSIMDVGFEKVYLMQNPLNFEASDVISTY
                     VYRTGLIDADFSFSTAVGLFNSIINFIILIIVNMISGKTSEISLW"
     gene            22570..23469
                     /locus_tag="PK733_05385"
     CDS             22570..23469
                     /locus_tag="PK733_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10009.1"
                     /translation="MYSIKETGADRAFNIINYVLLVIVLIIIIYPLWFVLIASVSDPD
                     LVNSGKVYLIPKRISFEGYRRIFQDGEILMSYKNTLIYTIAGTLLNLTLTLMCAYALS
                     KKKLYGRNVITFLFTFTMFFSGGLIPTYLVVKQLGLLNKIWALILPGAISMYNVIIAR
                     TFFQSTIPEEIEESAMIDGSSVTNTFLKIILPLSKALISVLTLFYAVGHWNSYFDALI
                     YLTNRKLYPLQIILREILIENQMKADMVAEGLMEESMINKVKYASLIKYGIIIVSSLP
                     VFMIYPFIQKYFVGGIMIGSLKG"
BASE COUNT         8241 a         3321 c         4330 g         7751 t
ORIGIN      
        1 cataatttca tagggaaccc gaagcccgct atttcaatag cggtgtagtt cacttatgaa
       61 tactaaagag catataaaga atcattttat ggatgaaaca atttgccaga cttccatagc
      121 taccgaaatg gaagtatcaa ccagctatct ttcacgtatt tttaaacaat atactaatca
      181 aaatatggtt gattacataa atgaactaag ggtggaaaaa gctaagttgc ttttagaaaa
      241 tgagaagctg acgctatgtg aaattgcaaa aatggcagga tgtagtgata tatcattaat
      301 aagaattttc aaaaaacatg agggtgttac accaggtaaa taccgggatt atgtagcatc
      361 aaaaagggca gatatggcag attaattatg ttttgttatg ctataagcgg ttatttgcag
      421 ccattctcca catataaagg ttaaatctaa gcaagtatca actttataga gtaataaata
      481 aataatcaca gatagagagg aagttactgc aaatgagtta tctaaataaa tcagatatta
      541 ttaatggctt gaggaaatta ggtttaagtg atggtgacca tgtaatggtg catagctcct
      601 taaaaagttt cggatatgta gaaggaggtg caaatacagt aattgacgct gtacttgaaa
      661 caatagggtc tgatggaact atgctgttgc ctactaatgt atttcaaggt tcagtaacag
      721 tttttttaag agacaaaata agagacaata agggaattga tcttcgagta gaaaaatcta
      781 tgacaggaat aataactgaa actctaagga atagagcaaa ttctttaaga agtatacacc
      841 cttcccatcc ggttgcagct attggcaaaa aagcagaaga acttctttca gaacaccata
      901 ttggagattc tcctgcaggt aaaaagagtc cttatggcaa attagcggag cttgataagg
      961 gtaaaatact tttacttgga gtaacaaact ctagaaacac ttccatacat actgcggagg
     1021 agtattattc ttcatatata ttcataggtg aaacttttga tacaaaagtt atagctttag
     1081 atggaaaaac acaccatgtt aatgttaaag gatattgtgt aaacacacca agagatttta
     1141 ctgcacttga tgatgagcta ataaacaaaa gtataatgtc aagaggatta attggtaatt
     1201 ctattgcagc attaattaat tctaaggatt tgcttagttt tatgcgggaa gagataggta
     1261 agaatccgtt aattctactt agccataaaa ataatacata gcgggtatat tatgaggtat
     1321 gcatgcagtt aatggacagt gagccagggc tgcaggctgt atatctagca gtgtctagtt
     1381 tggtggcata taaaatctat aaaaaataaa taggaagcaa ataacaatga aaccttgagc
     1441 aatacgtaca ttatgagaag atcaatcaat tataatatta atgcggggaa tttaacgata
     1501 acaatataaa aagctttcat ctactcaaag gaacgaaagc aggaggagat tatgaagaac
     1561 agttttattg atttattcag gcagccagat gacatattgg ttcagacaca ggatagtggt
     1621 atacgctttg aagaacctaa ggaacgtacg gtttctccca ctaaaattga gtatgtatat
     1681 tcggataaag aggctcgcgt aattgtccat ccatgcaaga taccgcttaa gcgtgttaag
     1741 cttcgctgga ataataggaa ttttgaaatc gaacgtgtac tgaacgattg ttttgacagg
     1801 tgtacaaaca atttggcgtg ggactgcatg aaacctgatc gtgagttacc gtggtatttt
     1861 gcgacttacg atggcgaacg aacccactgc tatggtgtaa agacaggttg taactcttta
     1921 tgcttttggc aaattgatcc ttatggtgta tcactttggc ttgatatacg taatgggggt
     1981 aatggtgtag aactgattga accattgata gcagcaattg ttatcagccg tgagggagaa
     2041 gagggagaaa cagcatttac agcgtgtcaa gaattttgca agataatgtg cgatatgccc
     2101 aaaatgccta agcagcctgt tttcggtgtg aataactggt attgggcgta tggaaatgtt
     2161 acttatgaga gtgttatgaa ggaaacagct tatttggccg agcttgcgtc tgatgcagtg
     2221 tgtaagccgt atatggtaat cgatgatggc tggcagccgc taagacacaa taacaactgg
     2281 ggtaactaca taggggggcc gtggaacggg tgtaatatgg gatttaccga tatggaaaag
     2341 acagcagcgg atatctctga atacggctgt attcctggtt tatggttcag agcacttctt
     2401 acagccaagc gtctgccgga tgaaatgctt cttaagaggc cggcggcagt gatgaactgc
     2461 gggcaggtta tggaccccag taatccagaa acattgcatg aggttttcac tgatgcaaga
     2521 tgtatttcag actggggctt caagctgata aagcacgatt tttcaacgtc cgatataact
     2581 ggcgggatca tagcttctga tggctggaat ttctatgaca ggacgaaaac cactgcacag
     2641 ataaccaaga aattctatga aactatacag cagggtgcga atgatgctat agtaattggt
     2701 tgcaatacat acagccattt gacggcgggt attcatgaaa ttcatcgttc tggcggagat
     2761 acgagcggca ggctttggga gtacacaaga cgtaatggcg taaattcctt catgcgtctt
     2821 gtacaaaacg gtacattcta taacatcgat cccgattgtg cagcctttac tgagagggtc
     2881 tccaatgaac ttaacattaa atttttagaa ctttgcgcca taacaggcag caccacctta
     2941 gcgtcaatta ctccgggaat tctgaatacc gaagaaaaac gcaagataag ggagatatat
     3001 aagatagcat cgatgaaagg taattgcgca gagccgcttg actggcttaa taccagctgt
     3061 ccatcaagat tcctatataa agacaatgaa tatttctttg attggtatag tggatataat
     3121 ggggtaagaa tgttcccgac atggatggcg taattttcat aattaaaaat atagaaggag
     3181 taagtgatat tccttgaata tgcctgagat atgaaaatgg acaacatgca tgtaaaaact
     3241 gctcttcttc attactgaag aagagcctta cttgagagtt tgcattatat acggattttc
     3301 tatataatat aaaaaagcag agggggaaac tatatgtcat ttgatatgtt agaatataat
     3361 aggtcggatt ttgatgagaa agtatttagg tcagctccgg atgtattagt tgaagcagta
     3421 agtatgaagg gagtagctcc tgaaaattat catgctacta caatattccc tgaatatttt
     3481 aaagtaaaag ggaaatggat acttgctgaa aaaagtcgta tggactgtgt tgctgttctt
     3541 aaaagtaata atgagaaaca aatagacaaa attgaaataa aggaattccg taaattagag
     3601 ccgggagaca aagttatttt aggtagaaca gaagatggta gccagggaat atatgttcat
     3661 acatccggtt tctcgtcaaa gaaggattca acagattctt ttgcatttcg gactggaaag
     3721 tccagggaaa catcttactc aagagactat gatagattgt atgatctatt aaagtatgag
     3781 agagataatg gatatatagt gtgggtatta ggtccagctg tagtttttga tgcggattct
     3841 cgtagagcga tggttgctct aatagaaaat ggttttgctg atgcaatttt cgcaggtaat
     3901 gcagtagcca cccatgatct tgaagctgcg ctgtttcata ctgcccttgg acaggatatt
     3961 tatacaaaaa attatttgcc aaatggtcat tataatcata ttgacgcaat taataaggca
     4021 aggcgtgcag gttcttttga aaagttaatt caagaagaga aaatcaatga cggtatagta
     4081 tatacatgcc ttaaaaataa gatagaatta attcttgcag ggtcaataag agatgacgga
     4141 ccactgcctt cagttatttc agatgtatat gctgcgcaga attctatgag agaacatgca
     4201 agaaaagcca caacagtttt aatgcttgca actcagcttc attctatcgc aacaggaaat
     4261 atgacaccgt catatcaagt tataaatgga atggtgagac ctgtttacat atataccatt
     4321 gatatatcag agtttgcagt aaataagcta agagacaggg gcagccttga agtaagctca
     4381 ataattacaa atgtacagga ttttttagta catttggaac ggaacttagc aaaatgaaag
     4441 ggcagcggta tcggaacaaa aaaattatcc cggaagatat cgctttccaa ataggcatag
     4501 tagtattgtg atggtaatga tttctgaata cttatactat acctcaagat tgcaggatat
     4561 accatttttt atgcggaaat ccttaggggt aactccatac tttagcttaa acctacttat
     4621 aaaatattga gatgttgaat aaccaaccaa tgaagatatt tcttctactt taaggttggt
     4681 tgtcaacaat agttttttcc cttcttcaat tttgcaattg gacagaaatt ctacgaatgt
     4741 aatacctgta tattccttaa aaagcctgct taaatggttg gagcttatat acagggcatc
     4801 tgaaactgat ttaagggaca gttcttcatt gcgtacattt gtagatacat attccattac
     4861 tttatcgatt atttccttgt tcttagacat atgtttatta tcaatttcgt caaacaattt
     4921 atttataata tcaacaagcc atgttctcac tatgtttata tttttaagtt tcctaaactg
     4981 ggtaataata tcatcattac atttagttat attttcaata cttgcgccat tatcatttaa
     5041 atttttatca attggtaaaa taatccttgt aagtattttt ttgcataatc aatgtttata
     5101 cttgtagact ttatatagtt aattattgca tcaacatttt taatagattt gtttctttcc
     5161 ttagacttta gcgaggattc caaataccct acaagctttt tatacttgat acctatgcta
     5221 caatctttgt atagtttctc atcagctctg aatgttgttt gttcgggata aatatagcta
     5281 tattcaatgc ttgcagatgc agaattataa gactcaatta cattcataat attgtcgaca
     5341 tatgagccta ttccgatata tttcctgaat agctttgttg tattatttaa atttgcaaat
     5401 atttggcttg tcaagatttc tattaattca taatatttta tatccgtgtt aatgaccgca
     5461 aggatatgat cgtcaacctg cccgcataaa caaacactgt tattcatatt aagctgtttt
     5521 tcaattgtat taacaattgt taacttttca tattcaagtt ctaactgggt ataatttcta
     5581 tttacttctg taccactcaa tattataatc actgctaaaa aattcttata tggaaagctt
     5641 atgtttaaaa gctccatctt atcattgatt tcctgattgt ctaaatattc attagttgtt
     5701 agcattctaa taaaattaat ttttaaaatc ggatagcttt tttctatttg aagtttatgt
     5761 agttttagtt catctgacat atcagtgact atcgaactta ttgtattgag actatttccg
     5821 ggttttttaa tattagaaac atttttacaa aaatccacca accttaccaa tggagacgaa
     5881 actttcttcg ctgaaaacac agatacaatc aacccaagga agattgtaag aaatgaagtc
     5941 agcaatattg tatttaacaa aaagcctata ccattgttat attgggctaa gggtgttaca
     6001 tttatatatt tccagctatt ataggctgag gttgtgtaag aaacaacaga ttgtgtacct
     6061 gatgcattac aaagaaaata tcctgaattt ccttgagaag taactacatt ttttccaaaa
     6121 tcaaaatcgc atatgttttt acctatgtat ttactatgag tatgtgaaat aatcgtacca
     6181 tcataatcaa caataataat attatccaaa cttgatggag ctgatttttt tatatttttt
     6241 ctcaaaacat tttcatctat tgatattata ataaccccac tattatttac ttttcccggg
     6301 atttttctga taaagtgaat catgctttga ttagtttcat ctttaagcaa atacttaaat
     6361 gggtaattat cttcgaaata ccaatatata ttatttgaaa agtctataga gtctactata
     6421 gcttgataat aattcagttc atcattattt atatattgag gttcctgata aagagtgtgt
     6481 cttatacaat ctgttgaaac aagaaggtta ttgtatttat aatatatggc tatcttggaa
     6541 gatatagtat tcactgattt tatagagtct acatattctt taattttgtt tacatcaact
     6601 acatgacctt ctaagggatt cgaaaaataa aatgaaagat ggctgttagt gcgacaatcc
     6661 tgaaggattt tcaaggatgt atcattaata ccttccaata gaaaattatc aatttgattt
     6721 ctaaagtgaa taagtgtttc tttatttaat tcaattgtat gtttttttat attatttcca
     6781 atataattat aagatagtat tcccataata caaatacaaa caacaattat cagccaataa
     6841 gagattataa ggtttgctaa aaaagacgat actttttctc tgcggggaaa ataattgttt
     6901 aatctaggga aaaaagtctt aagcatttga tgattaacac ttgaagtttt ttctaccaca
     6961 atccacaccc cttttgtcta aatacgttga atgtactaaa aaatatgctt aaaaatataa
     7021 atcggtaatc atgtttattt gtgtagtaaa ctatgaatca ttttcacatt atatataata
     7081 ttattataat atgagatgcg gcatgttgca agctggatgg caattgaggg tgcataaatt
     7141 atatctgata cttgttttgt aaaaccatcc tgcatcaacc actccatgta caactttcat
     7201 gtaaagccac aatatcccag tagaaatggg tatggaaagc caattttacg tgacatattt
     7261 tctcaaaaat atatcagctt gcatttatta catctacaac cttatcccaa agcttgctct
     7321 catattttgt tctcaaagat gctatatact gacccccatt gatgctccat cgcattccag
     7381 actgcttcat cctttgctgt attaccatct tattagcact ttctattgct ccactaccaa
     7441 tataataccc ttttttctta aaagccttgt aatctattct ctcacggtta ttgtggatgt
     7501 aggtataaag gttcactact gtatccggaa ccttgtccac cttcttttct tctacaagtt
     7561 caaccgcttt atccactctt ccattagtca cagcatctag tacttcattt acccatttct
     7621 ttctgcctac ttcatcttca gggtatataa gttttgcgta agcatgagtg ttctcactaa
     7681 agtggtaata gtccagaatt tgcaccgcat ccggaaacaa ttcctcacac atattccata
     7741 tccactgtgc accatctcct attacaacca actctttcac tttcccgtaa cctgctcttg
     7801 ctgtggctgc aaacattagc tttttaaatt cattcacact acccaaatat gatacatact
     7861 cttttttcgt aataatatgg tctccgttct ctctcataat tatattccta tcacggaaca
     7921 ccattcccat cttcatttcc ttccatgtag acccatcctt gtcctccacc cgggtattta
     7981 cctgcaaccc atcggtaagt gcataaagtc ttccttcttc tcgatccttt ggcagttctt
     8041 gaggggctgc ttcttcaggt ttctcatagg cctttctggc tttttccata tcctccttga
     8101 agaccttctc tccaacttct tccgatacaa tctgcatttg tgttgggctt acttcaaccc
     8161 ctacagtctt tttcaatgct tcagatgccc tctcaaagga aggcaacaac tgcgacagat
     8221 acgtaattaa ttctgtcatt cctttagtaa tacgatgttt cctgtaaatc tccagttgtt
     8281 tatcaagtat cccataccct tcatgacacc tacggcaaaa cacactgtct cttactatcg
     8341 atatgtttcc gaacatagtc aatatctgtg tcttttcatc tgttcctgta ctaatcattt
     8401 tcttcccaca gtcgcagtac ctatcttttt ttttcgactt ctatgttact aagaagttgt
     8461 cctgacatcc ctactgctat ttttgcaaac ccgctcattg cttcccctat aagctgctcc
     8521 actgtattta ttgttacttt gccgtccgct tcttgcctaa taatttcttg cattaattcc
     8581 cttagaatcc cttcagcttc cggaatgttt ttaaacaaaa tttcgtttgt attttttatc
     8641 atgacctctt taccccctgt aatatattct atattggtat attaaggcct ttcactacta
     8701 tcttcaaagg taattccttc ctgtttacca tttttacctg cttttttgcc attataagaa
     8761 aaatttacat cagatatatt aaatgcaccc ggcaattgag caaacctcac aaatgaatga
     8821 aattgtatgt aagaaaggta aaaatactga atttcaggag tctaaaaggt tcaaaaaacg
     8881 ataattaatc tttattttat catataactg ttatcagaaa aaaataaaat aggggttttt
     8941 ttatatattt tgatggcaaa tcattattgc attatcaggt tttaagaaat agaattgctt
     9001 gtagggaata agcaatgcgc gttgatactt tccttattag aattttttta ctaataaatt
     9061 actaataaaa tcaattaaat aaaaagcgaa taataataat tacaggaggt actttatgtt
     9121 taatattaaa aagaagagtt gcattttatt ggctctgata ctggcattat caactattgc
     9181 agcttcatgt tcaagcaaag atagtggaac gagtaaaaat gaaggctcaa ctcagtcaga
     9241 cagcaataat tcatcagtta ctactgataa agaagtgaaa acagatccgc caacaaaact
     9301 taggtttatg tatagtacta ctggaacatc tgtacctgga gatgttgatc catgcagcaa
     9361 tccatttctg aatataatag cagagcttgc caatgtggag tttactgaag taatagtacc
     9421 gccatatgca gattacgatg ggaaattcaa acttactgta gcatctggtg acatgcctga
     9481 cgtaatgcat gaccaatgga gttttaacga agtcacgaaa tatggtatag atggggcatt
     9541 cgaaccttta aataactata ttgcaaactc atcatttatc agcaaaatat attctcctgc
     9601 tcaaattgat cttatgaaag cagttgacgg aaacatttat ggacttaagt ccagagcttc
     9661 gggggatctt acctgccttg aatacagaag ggatcttatt gatgaactaa acaatggagt
     9721 tgtacctgaa acaccggaag ggtggtttga tgtagcgaaa aagttgaaag caaagtaccc
     9781 ggaatcaatt cctttttcag ggcataaaat gcctgatgta tggatgttta gttcatttgg
     9841 tgtttcgtgt gcaggatggc aatataccaa tggggaattt attcattgtt ttgaagcccc
     9901 gttaataaaa gaatgtattc aatttataaa aaaggcatat gacgaaggtt tacttgataa
     9961 gaattttgtt acaaaccaaa cagccgatta tttaaatgtt atgtttaaca aaaatctact
    10021 ttgcgacgat tacacatatt taaatgttgt aggagatatg gcaaatgcat acgataggaa
    10081 tttaaatggc ttaatatttg cacctgcacc attaccaatg aaaaatgata gcagaataga
    10141 tatatcaaat gtatattccg gaccaggttt attaggaggc tcatcattgt gtgttgcatc
    10201 aggttcagaa gaaaaagaag gtgcattcag gcttatcgaa acattttgtt ccgatgaagt
    10261 tttaaatctt gcttcatggg ggagagaagg aatagaatat aatattgtta acgatgaaaa
    10321 ggttttggat cttgaaaaca gcacaaaaac agattatcgt ggtatgtatg gtattatgaa
    10381 gtattataac taccctgagc aaatcaaagt aatcaaagct catgtattta gctatttccc
    10441 tgaaattaag gatagtctaa acaaaattta tgacgatgca tgggatatac tagaagaaaa
    10501 attgaagtta gttcctgcag tgacacctca agattttttg aaactagaag ctgatacttt
    10561 atcacggaaa tctgaagcaa aggaaagagc agaatctatt atacttaggg caataatagg
    10621 tgaaatcagc atggaggaat atgataagca ggttagtgaa tttcttaaaa aatatcaatc
    10681 catcacaaat gagtataata aaaaggctga tgaaataaaa aaggactgga aatattaaaa
    10741 tagcataaaa ctattaactg cagataaata catgaaatat gtatttatct gcagttaata
    10801 ggaaaagtgt ataatgctta aaattagtaa ggagttaaaa tatggagcag ttggtaaaga
    10861 aagcatatgt taatttgaat aaggtgaaat ttaatccagg aagggatatt ccaatttatt
    10921 ttttgctctt accagcagta gtgttggtaa ttatttttaa ttatatgcct atatatggta
    10981 taattatagc atttcagaaa ttcagtgcat tcaaaggtgt ttttggcagt caatgggttg
    11041 gatttgacaa tttcaaatat tttttgctag atgaaaattt ttggagggta ttcagaaata
    11101 ctgtgataat aaatatatat caaataatat tcggttttct tgtacctatt atttttgcat
    11161 tgttattgaa tgaaatcaca cgaaataaat ttaagaaggt tatacaaaca gtgagttatt
    11221 tacctcattt tatttcatgg gttgtagctg ctgggatatt taactcaatc ttgtcgccaa
    11281 cgaacgggtt agttaatgtt attctgaatg agctttttgg aattaaacca atttattttt
    11341 tggcaaaaca ggaatatttt agatctatta tcgttttatc atgtatatgg aaaggatttg
    11401 gaatgagttc tgtgtattac cttgcatcat tatctgctat tgatactcag ctatatgaag
    11461 cttcttatat agatggtgca ggtaagtgga gacagacatg gcatatcacc attccgggtt
    11521 taaagagtat agcagtaatt cttttaattt tgcagatagg aagcatggtt tctataggct
    11581 tcgaacaaat atttcttatg tataaccctc ttgtttatga cgtgggtgat gtaattagta
    11641 cttatacatt tcggcttggt attgaacaaa ccagatatag tttgacagca gcaataggtt
    11701 ttactcagtc agtgattaat tttattcttt tgtatgcagc aaatatgcta tcgaaaaaga
    11761 taatcggctg gtccatgtgg tgataattta atcatattat ttgtaatatg tattggagga
    11821 ttgaaatgaa aaggacaaaa ggtgaaaaaa tatttgaaat atttgatatg attatattat
    11881 taattataat tagtactata attctcccta tcatgcatat aataagtgtt tcaataagta
    11941 ataaggaagc ggtattaaat ctttcgatag gtatttttcc aaaaggattt accatagaag
    12001 catataagga tgtaatatcc agaaagacat ttttgtcatc tttatgtaat tcaatatatc
    12061 ttacgatcct ttataccttc ttttctttgt tggtaaatac tatgtgtgct tatgcattca
    12121 ctaaatattt ttatgggaag aaatatttaa atattttttt tattattaca atgtattttt
    12181 ccggaggact aatacctaca tatattttaa ttacaaacta cttgaaaatg cataatacat
    12241 tttgggcaat gctattaccg ggaattgtta atgtatttta catgattatt atgagagcac
    12301 agattgaagc tatccctaaa gaattatccg aagcagctat tgtggatggt gcaaaagaat
    12361 atcaggtttt gtataaagtg attctacctg ctataaagcc tacaatagcc gctgttggaa
    12421 tgttttttgc acttgatatg tggaacatgt ggttttcatg catgttatat acgaataagg
    12481 agtccatgtg gacattgcaa tattttttga gaatgatggt gtttaataaa gatcttaatg
    12541 tggatagaag ctcgaaaata gcaatttcca tagataggaa taacattgcc cctgcgaatt
    12601 ttcagatggc tgcaattgtt ttggtcgcgt tacctatagt atctatttat ccatttattc
    12661 aaaagtattt tgtaaaaggt attttagtag gttccgtaaa aggataagtg agaaattaga
    12721 attaataggg gaaaagtggc agataacaat aatagtaagg gcttttctaa aaataaactt
    12781 aaaaaggggt tatagtaaca tgagcttaaa taaaaacaaa tacctaaata ttatagaata
    12841 tgataactat attaatgttg ataatgattg ccttaacatt aaaattaaca gaaaaacagg
    12901 cagtatagcg gaatttctac ttaaagaaaa aaatgttgat atactggatg gagaaatagg
    12961 atacattgca cttcattaca aaaccgcaga gcaattatgt tttactcctg atgatgtgaa
    13021 attgaaaagt tacttaataa ataaaagtga tgagagtaat gttttatata tgaatttagt
    13081 atcctgtaaa tgtgatacag aattaaatat tgaattaaaa atggaggatg gaaatatcag
    13141 attaaattgt actccaataa cttcccgacc taaaatgtgt gaagttgaag tagtctatgt
    13201 ccttccattt tttaaagcaa acgttaatgg ttttacacct tatagtgatg cggatttact
    13261 tccggaagag atagatcata agctgttcat atatggagga gatcaattca gaggtgaatt
    13321 aagcgatagt atatcattac ctttagcagg tttttataat aaaaacgaaa actatggaat
    13381 tactgtagtc caacctgttg atatatataa accccaagta caatattatt atgttaggga
    13441 aaatgctcct ttatcatttg tagtaaaata ttgtaattta aggttgtcaa aatatgattc
    13501 tcctaaaaca tcaataattt tttctgttca tgaaggtgat tggagatgct ctttaaaagc
    13561 tatatatgac ttatatccag aatattttaa tgtaaacaac aattctattt atgaaactga
    13621 aggtacgatg ttatgttcta ctttacagcc agaagaaaat attaaaaatt gggttttgca
    13681 gcaagacctt aaatggcagg aaattcatgc aaatattttc ccttattacg ggttatatgc
    13741 tcccgaagta gaggaatgga atgatatttc taatgtttat gatatatcaa aacttaatct
    13801 aaacagatta attgatattg aattttggga aatgggccat agtatgatga agtactcaaa
    13861 tgataagaaa attgcaaaaa aagatgtaaa caattatatt gaaatgttgc ataaatatgg
    13921 aataggtgca tttatatata taaatgcagt aatattaaat aaatacgaaa aagaaaagta
    13981 taaagattct gaagcaattg gacttgatgg attaccatgc ttcaaaaatt attatctaaa
    14041 taaccctatg aatcctgatc cgggtttgag ctgggggcaa aatatcattt accagataga
    14101 aaagatacta aactactttc cagagtgtga tggattgttt tttgatgaat tacatttcag
    14161 acattttgat atgaatcatg atgatggtat tacagctgta aatgacaaac cttcatatat
    14221 gattggtttt gctattgaaa aaataagtaa attaatatat gaaaaaattg aaaatagcgg
    14281 taaatccata tggggcaatg ggccaacgag ccttgaagta atgaggtata ttgatggctt
    14341 aatggctgaa aatagttgga aatggttggg gacagtccaa tattatgcat taaacaagcc
    14401 tatcgtgtta ttacaaagca gaacggaatc tataatagaa ctcgaagaaa ttttgaaaaa
    14461 tgcatttatt tgtggttccc aggtgcatgt tgtatggaag cccgatagca caagaggtac
    14521 tatggaaaca ggagaaatgc ttcctgataa tgataaagag aaacttgcct tattagctgg
    14581 atacagtaaa atattaaaac tgttaaaaaa gcgttcatgg tatcttaaag cacattgttt
    14641 aaatgtttta gatgataatt ataaaggtaa catattcaaa attccatctg gagggtatgt
    14701 tgtaacattg ataaataaaa agcatgctag ctgttatgaa agtgttgaaa ttgagctcct
    14761 tttagatgat gattttactg ttaataaaat ctataatgta gacccggaaa gtgatgaaaa
    14821 cacaaagata gaatggggaa aaattgccaa ttcaataaat ggggtttata ttaaattaaa
    14881 aataaaaaac tcaatagcta ttgtaattga ataaaaaaac tcaattattt ataaaatgaa
    14941 aggtggaatg atttatgcaa attattgatg cacatcagca ttattatatg gaaccgcaaa
    15001 ctattgagaa cttgatagaa aaatcgaaaa tggcagacat aactaaagtg tgtttaagcg
    15061 ggttgggaga ttattcatgt gataaatata atgaagatgt tagaaaagct attgagaagt
    15121 atcctgatac aattatcggg tttggattta tacgccttgg aattgataca tggcaccttg
    15181 tagatgaatt atttgaacag ggattcagag gattgaagac tattaatcca agaaaaaatt
    15241 atagtgataa agaatattat cctatatata aaagagctga ggagttaaat atgccaatac
    15301 ttttccatac aggtataaaa atatttagtc cccaagatgt aaaacatgat gttgcgtgtg
    15361 aaagaatgcg gccaattttt cttgacgctg ttgcaagagc attccctaga ctaaatataa
    15421 tcggagcaca tctgggtgta ccttggtata acgaggcatg tgcagtttct gaagagaacc
    15481 ctaatgtata tttcgactta tctggagcaa taaatatact atataaattg gaaccatggt
    15541 tttttaaaat cttgacatat tgggaaatgg cgaagggaaa gtttgttttt ggagtagatg
    15601 accattatga gagaatacct gaagctgtta acaagtttaa tattttaatg gataaattaa
    15661 atttttcaca ggagttaaga gaaaaaatat tttatggaaa tatggaaaag atactcgggt
    15721 tataagaggt gaaatggtaa tggtaatgtt tacagctaaa gagaagctta aaaatggtga
    15781 atggctaaca ataaaagcaa taaaatctcc tacagcagaa tatgaagaca ggatattctc
    15841 ttacataaat cctaaaatgc cttggagaca ggatacatat aagagaataa acggtgatat
    15901 tgccgatcaa tgtaaagaca tgttttttat aggagaaata gatggggaaa ttgtttccct
    15961 gatgtggtat acggtatccg atgatgttgg tacatatggc atggtatcta cttctgaaaa
    16021 gcatagaaat aaaggcatat gcggatgcat tatgaagtat tgcatacaat tcatggatag
    16081 tgagccgggt ttacaagctg tatatcttgg agtatccaat ccgatggcat ataaaatata
    16141 tgaaaaaaac ggatggagac catacaataa tggaaccttg aatgatacat gcattatgag
    16201 aagattaata aattgcaata ttgataaaaa agaatttgat gataattatt ataaaaagta
    16261 tcagaatgac gggagcagga tgataatccg aagggttatc cggggtgatt tgcctaaact
    16321 tgaagcatta tacaacctgg ctgaaagtac tgttatgata aaagattata atgcatcaat
    16381 ttacataggc acagctgtag aatgccaaat cattgatttg ataaataaaa tggaattagg
    16441 ggaaggactt ttttattgta ttgaaaatgt tttaggacgt atagtcggtg ctgcaacact
    16501 ttataataaa gaaaatggat acagaacagg aagtgacaat atacacatta atataagcag
    16561 taatagaatc ggtaatgaaa tcagcggtgg aatcagtaat gaaatcatta atacaagcag
    16621 taatgaactt ggtataagag tattggattt ttttgttcat ccaaactatg aggaatacac
    16681 aaaatcatta gtaggagaag tatcggctaa tataaattct actctggaga gctgctatgg
    16741 ggatttgtag aaatagtgaa tattagaact gagaagaggt aatatgagaa cctgggagtt
    16801 tgcattattt gcagatgcag attttctata gaatataaag aagcaaggaa caatatttat
    16861 ggtttgctag tgtttttaca tatcaataca gaatcttaca tatttacttt atctctagac
    16921 acacctacaa tgagagctaa gtgtataatt atagatacaa aaattatacc aattaaatct
    16981 gtagtttagg gatcttaatt tatttatcta aatactgaaa ggggtagcag tatgtccaat
    17041 tatattagag taagttcatt aggtagtcca ggggcacttt atgcagaagg agaaactgat
    17101 tatcagaaac tggttgataa aatgaaattg atgtggcagg aagaacttga aaaggtgttg
    17161 ccggataagc cggacttaat tgtattaccg gaatgctgtg atgaattctc agggctctct
    17221 tctaaagaaa atattgaata ttatagagtt agaaaagatc agataagaga ttattttatg
    17281 aaaactgcaa aagaaaataa ttgctatata gcatactcgg caatgaggta tatacctgat
    17341 ggctcaataa gaaactccac acaaataatt gatagggacg gcaatatagc gggaatttat
    17401 aataagaatc accctactat aagagatcat gaaattaacg gcactctcta tgggaaaaat
    17461 gctaatgtaa taaaatgtga ttttggtaaa gttgcttgtg taatttgttt cgatcttaat
    17521 ttcgacgagt taagacttaa atatgtagaa caaaaacccg atcttgtaat attttcctca
    17581 tattatcacg gaggtttaat gcagaattac tgggcatact cttgccgaac atattttgtt
    17641 ggatgcatta tgggtctgca aaatactatt atttcacctg ttggagaagt agttgcgaga
    17701 agtacaaatt atttccatca tctatctcat aatataaatc tggattatat agtttgccat
    17761 cttgattata actggcacaa acttgatgca ataaagaaaa aatatggaac caaagtaaat
    17821 atatatgatc ctggctattt gggttctgtt cttgtttcaa gtgaatctga agatttagca
    17881 gctatggatt tggcaaaaga atttgaactg gaattacttg atgaatactt tgaaaggtgc
    17941 cgaaatcata gaagtatgcc tgggaagatt gaactctaac tatgaaggag gatagttatc
    18001 ttaagtagta gtgtaaaagc taattgcatt aggtacatgc aaattaatat aagaatatat
    18061 tgaagtaaca atcagaggaa ttatttatag tgaaaaaaga tataataaaa gtgttaaata
    18121 agatatacag taaaattttc gggaaaatat attggaagga tataggatgc ataatcattg
    18181 gaactctcac tgttgctttt tgtattaatg tgtttttagt tccgtacaaa attatagaag
    18241 gtggtgttac cgggattgcg attattttac attatattag tggagaaaga ttaccggttg
    18301 ggttaacagc ttttactatg acattgcctt tatttattgc aggattttat gtgcttggca
    18361 agagtgcggg tttcagcact attattgcaa cagtattgct ttcattattt atagatgtta
    18421 tatcccctat ttcaaaagcc attgtataca ggtattttat ttcacctgat gaaatactga
    18481 attatcctgt gattatgctg ttttattgtg ttattagtgg aataatatta gggacaggtt
    18541 atggaattat atataaaaca ggtgcttcca cgggaggatc agaccttgct gcgatgataa
    18601 ttaataagta cctgccaaaa gttactcttg ggataataat tatatttctt gatggggcgg
    18661 ttataatact ttcgggagtt gtgttcaaaa gttttgaaaa tacaatttat gcagttttta
    18721 gagcatttat ttctgctggg gttgttgact atattcttaa atgcagcagg aaacaaatga
    18781 aaaacggtgt aaataatagt tattgaaacc ataaagtata aacgaaaaag taaagctaaa
    18841 atgctataat catataattt ttggcttatt acatacaacg tagagttttt acatgtttac
    18901 ttatactaag aatagtggta cctttaatat atgtttcgaa catatttata tgacaattta
    18961 tacaatatag gatatgtgta ataatatgtc gtaaaaataa ggatgatgat tgatgattag
    19021 tagagcaaat aagtttacca aactattcta taggtatctg gtttcatata tacttctatt
    19081 tttaattccg ttgatgttat ttggtactat aatttatata agctctttta aaatatttaa
    19141 aaatgaagta gaagactaca ataaaagcaa gttataccaa acgatggaat tattggataa
    19201 ccagcttaaa aaccttgaaa gaatagcagt aaatatatct ttcaacaaga acctttatcc
    19261 ttatgcaatt gaagatgatg attattggac aaacggtgat tttttggagc ttaaagcata
    19321 tcttgcaagc aatacaatta ataatgagat aattgatgag attattctat atttaaataa
    19381 taaggatatt attttcacat ccaggggtaa aacatcaata gaaacacttt ttgagaatat
    19441 aatacgtgta aatgctaaag atgcaagctt attgacaaaa aaaatacagt gcttggaaac
    19501 tcctgaaatt ataaagattt ccaaaagaga taaggatact tctttattgg tgcaggaaac
    19561 atatgatatt ttccagggta atgaaaaaag ttatatattt tatgcatatc ctgcatatat
    19621 gtacccaata cctcaggtta gaggtgatac atatggaagt gtagtgttca ggataaactg
    19681 ttcaaaacta aacgatataa tgaaggcaac attaggtgat atggttgggg aaatacacat
    19741 tttcacaaat gattttaatg aattatcaag tattgaaagt gataagctgc tatcctcaaa
    19801 agaaataaag gatgtattga gcaatagtaa taacagacaa ataagttatt ttgaaggtaa
    19861 atatggacaa caatcttgca gcacaatgta tatagataat aaaatatcag taattgatat
    19921 ccaatccaaa ttaacaggtt accattatgt agcagttttg aaggttatag attactttaa
    19981 taaagttgca aaaatgcagc aaaagatact tgctctttta atagctgttg ttttggctgg
    20041 tatagtaatg tcaacagtat tggccttcaa gaattataga cctgtatggt cgttaatgaa
    20101 attgatgaat aatataagaa gtaataaaaa aaataataaa gatagtgatg ttttgtatta
    20161 ttttagaaat gaatttgacg aaatagaaac atcaataaat aatgtgctgg aagaaaacag
    20221 gctgttatct gataaggtaa gcttgcagcg gccttatgta gaagatcaga ttatatcaat
    20281 gcttttaaaa ggaaatatac gaaataaaaa taaagcaatg gaaatactct ctctatatga
    20341 tattgaaatg acaaacctgt attttgccgt attagtatta tcaatatgca gcaaccgtga
    20401 aagcgtaaat gatatttcat ttagtgaatt gtcctattgt ataaatatat tttttgaaca
    20461 aaactttagt ggaagcttct atactttaga aatggcaaaa ggaaatgtag tagttacact
    20521 gaattcagaa tatgatgatt tattaagaga taacttacaa gaattattaa ctggattaca
    20581 agcactttta tatagaaaca taaaaataaa aattaatgtg ggaattggca gaatatatgg
    20641 cgatatactg gatataagta cttcatttaa tgaagccatt tcttccattg actacagtat
    20701 gattaaagga ccggagagtt ttgtattttt tgaagatatt attaacagag atgataaaat
    20761 ttactggtat cctttggaag aacaaacacg tcttattcaa tctcttaaac aagggaatat
    20821 tgatatgttt actgatgtat tggcaaaagt ctgtaacata ataaaaagta aaaatatttc
    20881 cgcaggaatg attaagtcaa tttgttttgg cattattaat tgtattataa atatagttaa
    20941 tgagcttgaa accgatgaat ttaccaatga acttgaagat atgatggagt tcagaagcct
    21001 tgaggattta caaaacaaat tggaaaagat ggcatcaagt atatgtaact acattaatga
    21061 aaagaaagaa tgtggaaatg aaattttaaa ggcagctatg ttggaattta ttcataacaa
    21121 ttatgcagaa aacgggttat gcctggagat gctggctgaa gagttcaatc taacagcaaa
    21181 atatttgagt aaaatattta aagatcaggt tggctgcaag tttgctgatt atgtaaggca
    21241 actaaggata aagcatgcaa aaaaattact tacagataca gacatgtcta taagagaaat
    21301 tgttaatgct atagggtatg gagatacagc taactttatc cgtacatttc gtttattcga
    21361 aggagttact ccgggggatt ttagaaaaat taaaaaacct tctaccattg aatattgaag
    21421 atattggtta tattacatat taaaaaggat tattacatat ttacaaaatt ggcacttaaa
    21481 ccctataatc atattatgat atatgaacat gctataaaaa ataagcatgt taaaaaaata
    21541 ctgatttcat tattttcata tttatgaagc agataaatcg gaggggacat aatgagagca
    21601 acgagccaag cctcgctatc caaaagtgat aatatacttt atagaaaaaa aaataagagt
    21661 ttcagtaaaa ttcttaaaga aaattacgaa ttataccttt ttttactgcc tgcatttatg
    21721 tattttattg tatttagata ttggcctatg tatggattac agatagcatt caggaaattc
    21781 aatggtgcta tgggcgtatg gggaagtcct tgggttggat tgaaaaactt caggaatttc
    21841 tttgattcct attatttctg gatacttatt aaaaatacaa taggcattac attatattct
    21901 attttagtag gctttcccat gcctataata ttcgctttaa tgcttaatga agtacataat
    21961 gataaattca agcattttat tcaaacagta agttatgcac cacattttat ttcaactgtt
    22021 gttcttgtag gaataattat cgcatttcta tctccaacaa caggtgttat taatcacata
    22081 attgttgcac tcggagggga agcaagacct tttatgataa tggcaggctg gtttaagaga
    22141 atatatgttt ggtcaggtgt atggcagaat atgggctggt cagccataat atatattgcg
    22201 gcactatcag gtatagatcc acagcaacat gaagcagcag taattgatgg agcaaacaga
    22261 tttgagagaa tatggcatat aaatatacct ggaatattac ctacgataat agtactgttt
    22321 atcttaaata tgggttctat tatggatgtg ggttttgaaa aagtatattt aatgcagaat
    22381 cctttaaatt ttgaggcatc tgatgtaata tccacctatg tttacagaac aggtttaatt
    22441 gatgctgatt ttagtttttc tacagcagta ggtcttttta attcgattat taattttatc
    22501 atacttataa tagtaaatat gatttcaggg aaaacaagcg aaataagctt gtggtaatgg
    22561 aggtactata tgtattcaat aaaggaaact ggtgcagata gagcatttaa tattattaat
    22621 tatgtcttgc tggtaatagt ccttataatt ataatttatc cgttatggtt tgtcttaatt
    22681 gcttcggtaa gcgatcctga cttggtaaac agcggaaagg tatatttaat ccctaagaga
    22741 atttcttttg aagggtatag aagaatattt caggatggtg aaatattaat gtcatataaa
    22801 aatactctaa tatacactat tgctggtaca ttgctaaatt taacattaac tcttatgtgt
    22861 gcatatgccc tatcaaagaa gaaactgtat gggagaaatg taataacatt tctatttact
    22921 tttactatgt tttttagcgg aggcctaata cctacatatc tggttgtaaa acaattaggt
    22981 ctattgaata aaatatgggc tttaatttta ccaggggcta taagtatgta taatgtaata
    23041 attgccagaa cattttttca atcaacaata ccggaagaaa ttgaagaatc tgcaatgata
    23101 gatggctctt cggttacaaa tacatttcta aaaatcatac ttcctctatc aaaggcctta
    23161 atatcggtat tgactctttt ttatgccgta gggcattgga attcatattt tgatgcttta
    23221 atatacctga caaataggaa actctatcca ctacaaataa tcttaagaga aatattaata
    23281 gaaaatcaaa tgaaagctga tatggtagct gaaggattaa tggaagaaag catgataaat
    23341 aaagtaaaat atgcaagttt aattaagtat ggtattatta ttgtttcttc gttacctgta
    23401 tttatgatat atccttttat acaaaaatat tttgttgggg gtattatgat aggctcctta
    23461 aaaggttgac atttattatt tagattttat tgtggcagca aacctgggaa ttaacaattt
    23521 gaaaatagga ggtgagcaac aataagaggt gaacatctct gtgtcaatag gattattatg
    23581 gtttgtaaac cacagaaatt atagaatatg ataactcgtg atagaaaaac atagtactca
    23641 tag
//
LOCUS       DAOSDD010000039        23558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_963_length_23558_cov_4.791346, whole genome shotgun sequence.
ACCESSION   DAOSDD010000039 DAOSDD010000000
VERSION     DAOSDD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23558
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_23558_cov_4.791346"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PK733_05390"
     CDS             <1..765
                     /locus_tag="PK733_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10010.1"
                     /translation="WECPPDEYDPAYNYCINVNAADIRGPISFYRCLSYAERAPEKFI
                     LGYAPDPRRPDAIDSLKAAVEIHGVRVCGELKLRMMYDNPDAIRMYRYCGEKGLPVTV
                     HIDYEFSTGRNYPRPNWWYGGGIDAFERAIKACPETNFLGHAPGFWSHISGDDKYDKE
                     GYPKGKVVPGGKLIEMLRKYPNLYCDLSAGSGCNALSRDPEFAKGFLLEFQDRVLYAR
                     DYFDNIHQEFLNSIGLPADVLEKIYSGNALKLVPEK"
     gene            998..2287
                     /locus_tag="PK733_05395"
     CDS             998..2287
                     /locus_tag="PK733_05395"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOJ10011.1"
                     /translation="MKKNALVAQSGGPSPVINASLQGVIDACLSHNNHIENIYAAWHG
                     IEGVLTEELIDINSQSRQEIELLRYTPAAGAIGTCRYKLKENQTEDFERIIEVIKAHN
                     IGYFFYIGGNDSMDTAAKVSMLAAEKGVELVVAGVPKTIDNDIGDEEFTIIDHTPGYG
                     SAARYWAYLVQNVNEENRGMNVSECVSVLQAMGRKSGFITAASRLGDPNREIPLQLYM
                     AESHHSLESLADNVSCQLRRTGRCIVVVNEGFDVGNLGEAYDGFGHIEYGASEISAAQ
                     VVVNYLNKVGLPARGQVTGQLPGVIQRSVSIHASKVDIDEAYQVGKKAVHIAINEGTG
                     WMATILRAPGNSYKAVFDKVPLEVVANSVRYMPSNWITKDGLDVTDDFIRYAMPLIGD
                     GWPDIKIENGIQRFARFDIKFIDKKLPDYLPVMFRYL"
     gene            complement(2278..3207)
                     /locus_tag="PK733_05400"
     CDS             complement(2278..3207)
                     /locus_tag="PK733_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10012.1"
                     /translation="MKSILNNKYNIHLNKASETFIKSIPFKIYNLEYNSFSGTSMHTH
                     DYMQIWYVKKGCVEHIMNNKSHILVKGTIFVLPPYVAHCVNPIEGEDVNIMGCEFVTD
                     FINENITYNYDNPYIQSSLFDFAYLEPFLVSIENIKPGLALTGIAQNKVESLLDEMLH
                     EFNNEKKYYEIHIKADLLKLLAIVLREYENRDVGNNNEVFEKYRGAINNTIKYINENY
                     NKDIGLEGASKMAMMSSAYFSFVFKQITGKTYVEYKNSLRLAKAIDLMKDPGCSITDI
                     CYAVGFNDTAYFSKIFKKDTGMSPRQYRKVFYK"
     gene            complement(3330..4214)
                     /locus_tag="PK733_05405"
     CDS             complement(3330..4214)
                     /locus_tag="PK733_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10013.1"
                     /translation="MIYLTFFIFSILRGLNAIAVKMAQKDYVKKINDSICFTILFSLF
                     QLIFLFLIPPWNGYTFRLDMLIYPACFSIFYLISYILLLTALKEGPTSLTNIVYSFQS
                     IVPIIVGLIIWKEHIGMFQLIGLILFIVVLFLFNQGSYSEGTDSKKVTVKWALLASSC
                     TLAVGIAVIFTKQYMLTFDGFIKEYLIMYNLVVVIIGIPYLLITAAKGKQKFLLDKKF
                     LFYTASPALITDITNIIYMLYITSFKSAFFFPLMSILNILSVVVFSRIILKERISKTA
                     YIGILLSFVALYLLGIRG"
     gene            complement(4668..6524)
                     /locus_tag="PK733_05410"
     CDS             complement(4668..6524)
                     /locus_tag="PK733_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HOJ10014.1"
                     /translation="MDSFFRSIRKFFTCLKRCLFGVKPGKKNIIYGKSAYNEYMDYYY
                     SNMNSIDDNDLDNFSNMDLDNFSIMDSDDLDSDDLNSDDFPHMPINSHNNSKKSSNRS
                     YRHDKQCLTCRVDPSNPDTVPKFIDNLTIPEVLKPNKTIFGIPYYEIEMRQVKQRLHS
                     FFPETKVWGYNGTYPGPTIETLRDRKIYVKWINKLPYRHLLPIDRTLHGTSDTPEVRT
                     VVHLHGANIEPDSDGYPEAWFTRDYRETGAYFSRKIYKYTNHQQAATLWYHDHAIGIT
                     RLNVYSGLAGFYIIRDYLEHRLGLPSGKYEIPLLIQDRSFNKDGSLLYPSEPAQPVPE
                     VYPSVVPAFIGNTILVNGKVWPYLNVEPRKYRFRLLNGSNTRTYTLSLSNGKIFHQIG
                     SDGGLLSEPVELSTLIIAPAERADIIIDFSYCSNESLALVNADPDPHTSQVMQFRVNI
                     PLANEDKSKIPDILYPVMPIDKKLASMNRELRVAGGVDSYGRPMLLLDNKMWDDPVSE
                     RIKLDSIEIWNLVNITAFPHPIHVHLVQFQILDRRPFDVNYYLSTGKIRYIGEAIEPD
                     DNEKGWKDTVRVTPGQVTRIIAHFKEHTGRYVWHCHILEHEDHDMMRPFVVE"
     gene            6915..7937
                     /locus_tag="PK733_05415"
     CDS             6915..7937
                     /locus_tag="PK733_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ10015.1"
                     /translation="MIQSAPWSTDKFNGMPYRFIGKSGLQASNIGLGTWKFGYPETGD
                     DSRVDEKTALQIFDRAVETGVTFWDTANRYNNGTGNSEKIIGKWFKSNPDQRRNIVLA
                     TKVFGGMDGSTPNHSGLSRGNILDSVYACLKRLQLEYIDLLYFHAFDPVVPIEESLCA
                     IEDLVKRDLVRYFAVSNFTTAQLSMYRLAEEKTSVRCKITAVQNQFDILNGESEKQKG
                     VLEYAASSGISYIAWSPLARGLLTERYIDIANIGPGDRLYDEGSSDLYRDEVLRRKLA
                     GLAELSAKWGIKLNQLALAYMLTLPGMGPLIPSSSTIEQLESNAAAGKLTLDEAQLGL
                     IKNILR"
     gene            8046..9041
                     /locus_tag="PK733_05420"
     CDS             8046..9041
                     /locus_tag="PK733_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOJ10016.1"
                     /translation="MKILVTIPEGITRSTFIPADVAKLINDLGDVEWNTSSEQFTEEQ
                     LRDKLEGKDVCISGWGTRRFDQHVLEKAEKLKLIAHTGGSVAPIVSDYLYDSGVKVLS
                     GNLFYAESVAEGVIAYILAALRDIPYYSDMMTKGGWKSVDYYIEGLLDQTVGLVGFGT
                     ITKFVINMLKPFRTKIKVFSKHLKDETLAEYGIEKASLDEIFSTCKIISLHSAQRPDT
                     YHMIDKRLLEMIPDGALFINTARGSIVDEEALTEELAKGRFKAVLDVFEEEPLPDDNK
                     LRKMENVTLIPHMAGPTVDRRKFVTLGLIEDIKAFVDGKDMKNEISKEYAAYMTR"
     gene            9222..9854
                     /locus_tag="PK733_05425"
     CDS             9222..9854
                     /locus_tag="PK733_05425"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ10017.1"
                     /translation="MIQSKVKRERNSQKSKEDILNAAEIKFAEKGIYGTRVDEIAEAA
                     NINKRMLYEYFGNKEDLYRAVLIEVYSRLSKQEMILLSEDISSIDAMKRIISLYFKFL
                     KDNPTYVNLILWENLNKGEYIKDIDFSGIKDPVFTLLRKVINRGKEEGVFRADIDTEQ
                     VILSLLTYSFSYFSNRYTLSKLLGRKIDAEESIKKRVESVTEMFLRYLCV"
     gene            10047..10763
                     /locus_tag="PK733_05430"
     CDS             10047..10763
                     /locus_tag="PK733_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ10018.1"
                     /translation="MLWYNSTADEIKEANPDIAVLPVGSTEQHGPHLPVGTDFMIAGA
                     VAKGVAEKLGAYLLPTLPISNCREHMGKKGSIWMNPDTFYYVIKDILLSLKEQGFRKI
                     VYIQAHGGIFTGAPAIREVNATNPDIKVVKIDLVQYFTSDEMKDILECRDNLHACEFE
                     TSLMLYLQEELVKKDLIVDCVPDVPRDFLNYGSILKYSKSGVWGKPSLATKEKGEKLL
                     KLLVDKSVEYSNWVFKTFDL"
     gene            10927..11994
                     /locus_tag="PK733_05435"
     CDS             10927..11994
                     /locus_tag="PK733_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10019.1"
                     /translation="MNKKVRVLLVGAAFSADLHMDGYSRCRELADIVAICDKDLSRVK
                     ALAERYGVTDYTAYDDYKKAIDEVDCDLVDICLPNFLHHDVAIYAFQKGRHVISEKPL
                     ATTLEDAEAMVEAAKAADRKLYYAEDWVMAPAMNKAMEIINEGAIGKPLYIRARECHS
                     GSHSPFTQTIKYCGGGCMVHLGIHPIAFMLGLKDNKWTELVAMTSGGGENNLIHRTME
                     GEDWSACMMKFEDGTAALLEANYVTTGGMEDVIDIYGEKGCLHIDLTFSSPIKCFSIP
                     GVSYTVEKAEVTTGWSKPAVDEKFNLGYVNEIRHFMECCLKNEDAMVGLRGVDGLEAL
                     RVVNLIYKSAKEGISITNKNL"
     gene            12025..14676
                     /locus_tag="PK733_05440"
     CDS             12025..14676
                     /locus_tag="PK733_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ10020.1"
                     /translation="MRKDLYLPVEVGGITFKNPFYVASGPTTKSVKQLKRIEETGWAA
                     ASIKLTIDPAPYINRAPRYAMFNDRNALAFTAEKRLTFAEGLKLMEDAKKELKELILM
                     ANITYAGDKGVEGWVNMAKKFEEAGADIVELNMCCPNMSYNVELSSGDSKSCSIRTGA
                     SLGQQGDVVAEIVREIKKAIKIPLFVKLTPEGGKIARVAKSLYEAGADSVGGTANRLG
                     IPPIDLDNPGKAVYHLQDEISMSCHAGGWVKPLALRDTYEIRKVNGPDHSIMAAGGIR
                     NYRDAAEMVMCGADLLGICAETLISGFGFIENVISDLKNYMEEHGYKSLREMRDTIVP
                     LVKTAPELTIYKGNAKITEPNLAAPCKAACPHNVPAQAYVQKVAKGDFKRAYELIMSK
                     NPLQSVCAWVCNHPCEEACTRGEIGTPIPIREIKRFVIEYAKNAGWKPEINKKAERNE
                     KIAVIGSGPSGLSCAYNLALAGYKVTVFEKENYLGGMLRYGLPRFRMNHEVLDNEINF
                     LKDVGIEFVTGKGLGRDITVDSLKAEGYSAIYVGIGAQEGQSLNISGEDAKGIYPAIS
                     FLKSVYDGKAPNIGRKVVVIGGGFTAVDSARTARRLGADEVYIAYRRTRDEMPASAQE
                     IAEAEEEGIKVMYLVAPKEVVVENGKVAGIKMVNQVLGEKDDSSRRRPEAVAAAEFTL
                     PCDTVIAATGQKPEQSAVKGFTVDKKGMIVSMLSTGATSIEGVFAGGDVVNVESVIAA
                     ISAGKCGAVSIDKYLAKEAATLEYEPEYPVVSKEAVLKRSGYFKDNGPIDINTIDGKE
                     RIKSFTTYTRALTEEEAVAEAKRCLNCGCGEGCGLCASICSEFAIHLKSDDCWEINKD
                     ECVACGMCYNRCPNKNIEMINHNVLVK"
     gene            14887..15822
                     /locus_tag="PK733_05445"
     CDS             14887..15822
                     /locus_tag="PK733_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ10021.1"
                     /translation="MKKVLITPRSFPQSGKEAYKLLRENGFEIIDNTSGKSFDEDQMV
                     EQCANVDGVIVGVDPVTENVLKNAKKLKAISKYGAGLDNVDLKTAEELGIKVDRAAGT
                     NAVSVAELAVGLFFTLARNILYSAKSVKSGGWDRQRGVELTGKTVGILGLGNIGREVA
                     RMASGLGMNVIGYDPYVNKEDESVKKYSIKLVDKADVIKNADFLTLHMPLTDETKYII
                     NSESLNTMKPGAFLVNTARGELVDEDALYDALVSGKIAGAAEDVFSKEPPGEHKLLGL
                     DNFILLSHIGAFTKEANEKMAIKSAENLIRMLSEA"
     gene            15994..17499
                     /locus_tag="PK733_05450"
     CDS             15994..17499
                     /locus_tag="PK733_05450"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOJ10022.1"
                     /translation="MDLYMGVDIGTSGVRAALFDKNGKQLGLHHEEYPMICSEPGMGE
                     LDPERVFNSTINVVKLCIEISGADSKNIAAIGFSTQLFSILAVDSEGKCLTNVITWAD
                     KRSIAHADLIKDKHDYVKMYNSTGCRAQHPMYPVSKVLWLKDTMPEIFAKTHKLVSIK
                     EYVLFKLFGKYVIDITDASTTGYFNIHDFTWDKYIIDDVLGISPDILGEAVDCTFVLK
                     NMKKEYAEKMGISPETPVVIGSGDGMLANVGCGVFDNTSMSSTIGTSGALRIATEKPI
                     LDPLQRTWCYCFTRDTWVAGGAINNGGIVLKWLRDQHRAAYEAEAREADMDDVYQLFD
                     SYAMEINPGSDGLIFLPFLTGERSPNWNASTKGTIHGMQLIHNKKHLVRAAMEAVMYR
                     MFSVYEILTAFSDNVKLVLANGGYANSDIWLQIQADVFNREIAVAGIGEASAFGASYV
                     AMAATGAISSLRQPLPAMQPKKAIKPIRENSEIYKNAYKNFKDLYGRIYTK"
     gene            17560..18522
                     /locus_tag="PK733_05455"
     CDS             17560..18522
                     /locus_tag="PK733_05455"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOJ10023.1"
                     /translation="MNEILNKIGDYGIVPVVKIDDAKDAVPLGKALVDGGLPVAEITF
                     RTTAAEEAIKNISRELPELLVGAGTVLSVEQVKKAVNAGAKFIVSPGFNPDVVKYCVD
                     NNIPITPGCSNPTDIEMALSFGLEVVKFFPAESFGGISTLKAISAPYGMVKFIPTGGI
                     NAANINDYLSFGKVLACGGSWMVKDELIKKGNFQEITRLTREAVNTMLGFEIAHIGIN
                     SADAASYNDASEKLAAAFNFPRKEGEVSSFIGTAFEVMKSIGRGKNGHIAIKTNSVSR
                     AMAYLTRNGVAVDMDSVKNSPAGQPVFVYLADEVGGFAIHLTQK"
     gene            19003..19962
                     /locus_tag="PK733_05460"
     CDS             19003..19962
                     /locus_tag="PK733_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10024.1"
                     /translation="MGYNYIDGNMYEGVHIVRIEDNKNNLPFFIIKMGVCNQTKHMHE
                     FVQIVYMCKGKLKHVINNNTFDVYKGDIFIIPPYVPHYFIAPYDDKFELIEFEFIPEF
                     INEKFSVLQPGANSNNSKVIDSAFMDFAYLEPFLVSEHEVKPRLNLTGSVQIEVEKIL
                     NEVILEYEHRDIDFELIIKALLLKLLIIVGREYKKSTSGTEFDDILARHRDALYEALE
                     YINKNFTKEITIDEAAKVAMLSQSYFRYLFKQMTQKTFTEYINGLRIAKAAELLKNNY
                     EMKVVDICFEVGFNSVNHFNRIFRAETGFTPMQVRKGKMNTGQ"
     gene            20113..20847
                     /locus_tag="PK733_05465"
     CDS             20113..20847
                     /locus_tag="PK733_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOJ10025.1"
                     /translation="MKKPIIIAHRGASAYAPENTLSSFQKALEMKAGGIEIDVHLTKD
                     GYPVVVHDEKLGRTCNGTGFIKEKTLEELKELDFGSWFSPEFANEKIPTLKETMDLIA
                     DKNILFNIEIKNGPIFYPGIEKVVVDMVRMYNMSENVIVSSFNHYSLAEVKNIAPEIK
                     IGLLYSAGLYNPWEYAKMVGAEAIHPFYMSIHPEAVKMCNDNGILVNPYTINNPEHMQ
                     LALLAGVDGIITNYPDIALNIVEGVG"
     gene            20852..22174
                     /locus_tag="PK733_05470"
     CDS             20852..22174
                     /locus_tag="PK733_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ10026.1"
                     /translation="MKLDYRKTFVIGLGFFTVSIIWSIYNVAVPRYLDNLGLSGIMVG
                     TVMTIDNLFALIFLPAFGTLSDRTNTRYGRRMPYLLVGIPLSAIAFFLIPFSRESLPF
                     LMTTVILLNFFMSIYRAPTVALMPDFTPQPLRSKANGIINFMGGLGASVAFLMGGALF
                     KLKEYYPYVVGSSILILTIILMYNFIQEPKVVDEIMEKDKEKDTHKDTYNDKYEGTNE
                     YDTFNSREQGNALLDQKEAKKESGERLSLVFLLLAIFFWFSSFNVVETFFSTYGEKIL
                     NVDKSVSSYLLLTVSALFLVFSIPAGLIAGKLGRKRTILYGLIVIIITFISLIFVKTI
                     TLMYVLMGIGGISWALININSYPMVVEMTSNKGIGKYTGYYYFFSMLASIVSPILFGG
                     IKDILGDGFLFIYSTIGMIIAFFFMMLVKHGEAKVVVKSAFENLEHME"
     gene            22248..23000
                     /locus_tag="PK733_05475"
     CDS             22248..23000
                     /locus_tag="PK733_05475"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOJ10027.1"
                     /translation="MILMDLIKSASEIISASNNIVAFTGAGVSTESNIPDFRSAGGLY
                     NSKNKFNYPPEYLLSHTFFEEEPDNFFNYYRNNLIYRDAKPNNCHIALAKLEEAGKLK
                     AVITQNIDGLHQTAGSKKVLELHGSVDRNYCARCGKKYDLDFIMDLTLSIPRCKICGG
                     IVRPDVVLYEESLNERVLDNSLSFIAAADTMIVMGTSLVVYPAAGLLNYYKGDKLILI
                     NKTSTQYDYRAKIVINDSAGSVMKKILENIRL"
     gene            23221..>23558
                     /locus_tag="PK733_05480"
     CDS             23221..>23558
                     /locus_tag="PK733_05480"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ10028.1"
                     /translation="MLSWEELIIECSNCMKCELGKHRTNIVVGRGSRNAPLMLIGEGP
                     GQQEDKQGLPFVGAAGKLLDLLLDAQEITEDKYYICNIVKCRPPNNRVPFDEEAFQCL
                     PYLRNQVKLV"
BASE COUNT         7812 a         3734 c         4965 g         7047 t
ORIGIN      
        1 tgggagtgtc ctccagatga atacgacccg gcatacaatt attgtattaa tgtaaacgcc
       61 gcagatataa gaggccctat atctttttac agatgtctgt cttacgctga acgggctcca
      121 gaaaagttca tactgggata tgccccagac ccgagaagac cggacgcaat tgattcattg
      181 aaagctgctg tggaaataca tggcgtaagg gtatgtggag aactcaaact tcgtatgatg
      241 tatgataatc ccgatgctat cagaatgtat agatattgtg gagaaaaagg actgcctgtt
      301 actgtacata ttgattatga attcagtaca ggtagaaatt atccgaggcc caattggtgg
      361 tacggcggcg gtatagacgc ttttgaaaga gcaataaagg cttgccccga aacaaacttc
      421 cttggccatg ccccaggttt ctggtctcat atatccggag atgacaagta tgataaggaa
      481 ggttatccaa aaggaaaagt tgtgcctggt ggaaaattaa tagaaatgct aagaaaatat
      541 cctaacttgt attgtgatct ttctgcaggt tcaggatgta atgcactttc acgtgaccct
      601 gaatttgcaa aaggattctt gctggagttc caggatagag tactttacgc aagagattac
      661 ttcgataata tacatcagga attcctgaac agcattggac tgcctgctga tgtattagag
      721 aaaatctact ctggaaatgc acttaaactt gtacctgaaa aataaggcga atgtaaacaa
      781 atgtcgacca aacaaacgaa gcaaatgggg gcggttcttc tttaacacat tgaaatattg
      841 tgtaaagcaa caggaaccga ctctatttga ctacctattt aactatcaga ttaattggag
      901 atattactca atgtaaaaca gatattacct gaccgatagg gatactacca gctagaggga
      961 tgtgtgttcc attacttttt atgaaagggg attttttgtg aagaaaaacg cacttgtagc
     1021 acaatccgga ggtccgtcac cggttataaa tgcatcactg cagggtgtta ttgatgcatg
     1081 cttatcccac aataatcata ttgaaaacat atatgcagcg tggcatggta ttgaaggggt
     1141 acttacagaa gaactgattg atataaactc acagtctcgt caggaaattg aactactgcg
     1201 ttatacacca gctgcaggtg ctataggtac ttgcagatat aagctgaaag aaaaccagac
     1261 agaagacttt gaacgtatta tagaagttat aaaggctcac aatattggct atttctttta
     1321 tattggggga aatgactcta tggacacagc tgccaaggtg tccatgcttg ctgcagaaaa
     1381 aggagttgaa cttgttgtag caggtgttcc aaagactatt gacaatgata ttggagatga
     1441 agaatttaca attattgatc acactcctgg ttatggaagt gcagcaagat attgggcata
     1501 tctggttcaa aatgttaacg aggagaaccg cgggatgaac gtttccgagt gcgtatcagt
     1561 tttgcaggct atgggaagga aatccggatt tatcacagct gcttccagat taggtgatcc
     1621 aaatagagaa ataccccttc agctttacat ggctgagtca catcattctc ttgaatctct
     1681 tgctgataat gtaagctgtc agctaaggag aacagggcga tgtattgttg ttgttaacga
     1741 aggatttgat gtgggcaatc taggggaagc ttacgatgga tttggacata tagagtatgg
     1801 agcaagtgag atatcagcag cccaggtggt agtcaactac cttaataaag tcggtcttcc
     1861 cgcaagaggt caagtgaccg gtcagttgcc gggagttata caaaggtctg tttcaattca
     1921 tgcttcaaaa gttgatattg atgaggccta tcaggttgga aagaaagcag tccatattgc
     1981 tataaatgag ggaacgggct ggatggcaac aattctaagg gctccaggta attcatacaa
     2041 agccgttttt gacaaagtcc cacttgaggt tgtggcaaat tctgtaaggt atatgccttc
     2101 caactggata acaaaagatg gtctggatgt tacagatgat ttcatccgat atgcaatgcc
     2161 cttaatagga gatggctggc ctgacattaa gattgaaaat ggtattcaac gtttcgcaag
     2221 gtttgatata aaatttattg ataaaaagct gcccgattat ttaccggtga tgttccgtta
     2281 tttgtaaaat acttttctgt attgccgtgg ggacatgcct gtatccttct tgaagatttt
     2341 gctgaagtat gcagtatcat taaaccctac ggcataacat atatcagtta ttgaacatcc
     2401 tgggtctttc attaggtcaa tcgcttttgc cagccgtaga ctgttcttgt actcaacata
     2461 agtttttcct gttatttgct tgaaaacaaa tgaaaagtag gcggaagaca tcattgccat
     2521 tttacttgcg ccttcaagac cgatatcttt attgtagttt tcattaatat acttgattgt
     2581 attatttata gcaccccggt atttctcaaa tacctcatta ttatttccca catcgcggtt
     2641 ttcatattcc cttaaaacaa ttgccagaag cttaagcagg tctgctttta tatgaatctc
     2701 ataatatttt ttttcattat tgaactcgtg taacatttca tctagaagtg actctacttt
     2761 attttgagca atcccggtca gagcaagacc aggttttata ttttctattg aaacaagaaa
     2821 tggttcaaga taagcaaaat caaatagcga tgactgaata tacggattat cataattata
     2881 ggttatattt tcgtttataa aatctgtaac aaattcacat cccataatat taacatcttc
     2941 tccttcaata ggattaacac agtgagctac ataaggggga aggacaaaaa tagtaccctt
     3001 gacaagaata tgacttttat tattcatgat gtgttcaacg cagccctttt tcacatacca
     3061 tatctgcata taatcatgag tgtgcatgct tgtgccggaa aaactgttat attcaagatt
     3121 gtagatttta aagggtatgg atttaataaa agtttcactt gccttattta gatgtatgtt
     3181 atatttatta ttaaggatag atttcatata ttattttcct cccttcaaaa tgttatttct
     3241 tgtccttctt gcttttcttc tttctttttt acctcttcta tcattttatt ttatgatctt
     3301 ttgccaaatc cagtatacac tacccacctc taacctctaa ttcccagcag gtaaagcgcc
     3361 acaaaactca ataaaattcc tatataagct gtttttgata tcctttcctt taaaataatc
     3421 ctgctgaata ccactacaga aagtatgttt aatatactca tcaggggaaa gaaaaatgca
     3481 gatttaaagc ttgttatata aagcatataa attatatttg ttatatctgt tatcaatgct
     3541 ggtgaagcag tatagaataa aaacttttta tcaagcaaaa acttttgttt ccctttggca
     3601 gcggttatta ataaataagg tataccaata attaccacaa caagattgta cataatcaga
     3661 tattccttta tgaacccgtc aaatgtaagc atatactgct ttgtaaatat tactgctata
     3721 cctaccgcaa gggtgcagga gcttgcaagt aaagcccatt taacagttac tttctttgaa
     3781 tctgttcctt cgctgtaaga cccttgatta aataaaaata agacaactat aaaaagaatt
     3841 aaaccaatca actgaaacat acctatgtgt tctttccaga taataagccc cactataata
     3901 ggtacaattg attgaaagct gtaaactata ttagttagtg atgtaggccc ctctttcagt
     3961 gcggtaagaa gtagtatgta ggatattaag tagaatatgg aaaaacaagc aggataaatt
     4021 agcatgtcca gcctgaaggt atatccgttc catggtggaa taagaaaaag aaaaataagc
     4081 tgaaaaagtg aaaaaagtat agtaaagcaa atagaatcat taattttctt gacataatcc
     4141 ttttgagcca tttttacagc tattgcatta aggccccgca atatggaaaa aataaaaaaa
     4201 gtaagataaa tcattatagc ctcccgcagt ttttattaat ataaataata tcatatagca
     4261 gcgtatggat gcaatcgact aaactggtac gggtgcattt actaaaagtg aagaaatatt
     4321 taccaggagc tgtcgttacg ctgcttagag cctttaaatt cagctccggg caaaattgaa
     4381 atcatagaat tgtctgaaag aaaatcaaga aacaatttta ctcaaacaac aattttactt
     4441 atcctttgct cagattaaaa ggctctacag cagcttaact tatgctcctt accaatattt
     4501 tttcacttaa attaaatgca cccaactagt actgtataaa ttaggaagct atatatacta
     4561 aaagatgcca tgtaacgtac ttacaataca tggcattgca ggaaaaagtt tttgctgact
     4621 ttttaagaat aatagtagag aaccgtcccc atatttttat tttcatttca ttctactaca
     4681 aatggacgca tcatatcatg atcttcgtgc tctagtatat ggcaatgcca tacatatctt
     4741 ccggtgtgct ccttaaagtg ggctattatc cttgtcacct ggcccggggt tactctgact
     4801 gtatctttcc atcctttttc attatcgtca ggttcaattg cttctccgat atatcttatt
     4861 ttccccgtag aaaggtaata gtttacatca aaaggacgac gatcaaggat ttgaaactgc
     4921 acaagatgaa catgaatagg gtgagggaat gcagttatat ttaccaggtt ccagatttca
     4981 atgctgtcaa gtttaatcct ctctgaaaca gggtcatccc acattttatt atctaaaagg
     5041 agcattggtc tgccatatga atccacgcca cctgcaactc tcagttctct gttcattgat
     5101 gccagtttct tatctatagg cattacagga tataatatat cagggatttt acttttatct
     5161 tcatttgcta aagggatatt aaccctgaac tgcataacct gactggtgtg cggatccgga
     5221 tcagcattta caagagcaag actttcgttt gagcaatagg aaaaatcaat aattatatca
     5281 gctcgttctg caggtgcaat tattagggtg cttagttcaa caggttcact tagcagtcct
     5341 ccatctgagc ctatctgatg aaaaatttta ccattggata agctcaaagt gtatgtcctg
     5401 gtatttgaac cattaagaag cctgaaacga tatttcctag gctccacatt taaatacggc
     5461 cagacctttc cattaaccag gatagtgttg ccaatgaatg caggaactac tgatggatag
     5521 acttctggta cgggctgggc aggttcagat gggtaaagca aagagccgtc cttgttaaat
     5581 gatctgtctt gtattaacag cggtatttca tatttgccag atggtaatcc aagcctgtgt
     5641 tccagataat ctctgataat atagaatcct gccaatcccg aataaacgtt caaccttgta
     5701 attcctatag cgtggtcgtg gtaccagagg gttgcagcct gctgatggtt tgtatatttg
     5761 tatattttcc ttgaaaaata tgccccagtt tctctataat ccctagtgaa ccaggcttcg
     5821 ggatatccat cactgtcagg ttcaatattg gcaccatgaa ggtgtacgac tgtccgtact
     5881 tcaggagtgt ctgaagtgcc gtgcaatgtt ctgtctatag ggagcaaatg cctgtaagga
     5941 agtttgttta tccattttac atatattttt cggtctctta aggtttcaat tgttgggcct
     6001 gggtaagttc cattataacc ccatactttt gtttcaggga aaaagctatg aaggcgttgt
     6061 tttacctgac gcatttcaat ttcgtaatac ggtataccaa aaattgtttt gtttggcttc
     6121 aatacttcgg gaattgtaag attatcaata aattttggca ctgtatcagg gtttgaagga
     6181 tctacacggc atgtcagaca ttgtttgtca tgccgataac ttctgttact gcttttttta
     6241 ctattattat ggctatttat aggcatgtga gggaagtcat ctgaattcaa gtcatctgaa
     6301 tccaagtcat ctgaatccat aatactaaag ttatctaaat ccatattgct gaaattatct
     6361 aaatcgttat catcaattga attcatatta ctgtaataat aatccatata ttcattatag
     6421 gcacttttac cgtatataat attttttttg ccgggtttaa ctccgaacaa acaccttttt
     6481 aggcaggtga agaattttct tattgacctg aaaaacgaat ccaatgttta ccacctcttt
     6541 ttatatcgat actatttaca tcgatactat ttatatcgat actattttcc tttatacata
     6601 atatgtttac aaaattaagg agttgcaaat tattattggt aaaataaaaa ttatatgtac
     6661 ttttcactgc gcaaaaactg tttgacaaag atatatacat gtgttaagtt gtaactatac
     6721 agttattaac ttaatattaa gatgtttagt ttattaatac tactatgaaa atttggaaaa
     6781 ctatgactaa ggtggcatta agattgttaa gattttaagg gattcggcaa tgtagaaata
     6841 tcaataatat gctggaaatt taatggtaat ttttgattaa ttcgcttgga ttaatctgga
     6901 ggaggtatta taatatgata caaagtgcac catggtcaac tgacaagttt aatggtatgc
     6961 cataccgctt tattggcaaa tctggtctgc aagcttccaa tattggactt ggtacgtgga
     7021 agtttggtta tcctgaaaca ggagatgatt ccagagttga tgaaaaaaca gccttgcaga
     7081 tttttgaccg ggctgttgaa acaggagtta ctttttggga tactgcaaac cgctataaca
     7141 atggtacagg taattccgaa aaaattatcg gcaaatggtt taaaagcaac cctgaccaga
     7201 gaagaaatat tgtactggct actaaggttt tcggtggaat ggacggaagc acaccaaatc
     7261 acagcggact ttcaagaggc aatattctgg attccgtata tgcatgtctt aaacgccttc
     7321 aattggaata tatcgacctg ctttattttc acgcatttga tcctgtggtc ccaattgaag
     7381 aaagtctttg tgcaatcgag gatctggtta aacgtgattt ggtgcgttat tttgctgtat
     7441 caaatttcac aactgctcag ctttcaatgt atagacttgc tgaagaaaaa acgtctgtta
     7501 gatgcaaaat aactgcagtt caaaatcaat tcgatatact aaacggtgaa agtgaaaaac
     7561 aaaaaggtgt attagaatat gcagcttcat caggaatatc atatatagca tggagccctc
     7621 tggcaagagg cctgcttaca gaaagatata ttgatatcgc taacattggt cctggagaca
     7681 ggttatacga tgaaggaagc agcgatcttt atagagatga agttttacga agaaaattgg
     7741 caggtcttgc agagctttca gcaaaatggg gaataaaact caatcaattg gcgcttgcct
     7801 atatgcttac tttacctggt atgggacctt taattccttc atcatctaca atagaacagc
     7861 tggaatcaaa tgctgcagct ggaaagctta cgcttgacga agcccagctt ggtctcatta
     7921 agaatattct taggtaagtt gcttttattt agatgtaaaa tttattatga aatatttgca
     7981 ctataacaaa aaagaaaaac gagttgtttc agatggttaa agttaaagca tggagggaaa
     8041 aatttatgaa aatactggtt acaatacctg aaggtataac aagaagtact tttattcctg
     8101 ctgatgtagc aaaattaatc aatgatcttg gtgatgttga gtggaatact tcaagtgaac
     8161 agtttacaga agaacagttg agagataagc tggaaggaaa ggatgtatgt atttccggct
     8221 ggggaaccag aagatttgat cagcatgtac tggaaaaggc tgagaagcta aagcttattg
     8281 cacatacagg tggttctgta gcaccaatag ttagtgatta cttatatgat agcggtgtaa
     8341 aggtattaag cggtaattta ttttatgctg aatcggtagc agaaggtgta attgcataca
     8401 tacttgcggc tttacgtgat ataccttatt attccgatat gatgactaaa ggtgggtgga
     8461 aaagcgtaga ttattatatt gaaggattgc ttgatcagac tgtaggtctg gttggttttg
     8521 ggactataac taaatttgtt attaacatgc tcaaaccttt tagaactaaa ataaaagtat
     8581 tttccaaaca cttaaaagat gaaactcttg ctgagtatgg tatagaaaaa gcttctcttg
     8641 atgagatatt ttctacctgt aagattattt cattacattc tgctcagaga cctgatactt
     8701 atcacatgat cgataaaaga cttcttgaaa tgatacctga tggcgctttg ttcataaaca
     8761 ctgcccgtgg aagcattgta gatgaagaag cccttactga agaactagct aagggtcgat
     8821 ttaaagctgt tttggatgta tttgaagaag aaccgcttcc tgatgataat aaactccgaa
     8881 aaatggaaaa tgttacatta ataccccata tggcaggtcc cactgttgac agacgtaaat
     8941 tcgtaaccct tggtctgata gaggatatta aagcatttgt cgatggaaaa gatatgaaaa
     9001 acgaaatatc aaaagagtat gctgcgtata tgacaaggta gaggtccaga tatattttcc
     9061 atatttttta gtgtagaagg aaaatgcgcc ctcattaatc aaaatttatt gacattacaa
     9121 tatttacgaa gtataatgta actaaagagt tacttactta actgcttgtt aatttgctag
     9181 ttagtctgct tattaatttg ctggaggatg ttgattttat aatgatacag tcaaaagtca
     9241 aaagagaaag aaattcgcaa aaatcaaaag aagatatact taatgctgct gaaatcaagt
     9301 ttgctgaaaa aggtatatac ggaacaaggg ttgatgaaat agccgaggcg gcaaatataa
     9361 ataaaaggat gctatacgaa tattttggca ataaggaaga tttgtacaga gcagtcctga
     9421 tagaggtata cagcagactc agcaagcagg aaatgatcct gttatctgag gacatttcca
     9481 gtattgatgc tatgaaaaga ataataagtc tttatttcaa atttttaaaa gataatccta
     9541 catacgtaaa cctaattctt tgggaaaact taaataaggg tgaatatatt aaggatatag
     9601 atttttcagg tattaaagat ccggtattta ctcttctgag aaaagtaata aatagaggca
     9661 aggaagaagg agtattcagg gcagacatag atacagaaca ggtaatacta tcactattaa
     9721 cctatagttt ttcatatttt tcgaatagat atactttatc aaagcttctt ggtagaaaaa
     9781 tagatgcgga ggaaagtata aaaaagagag ttgaatcggt aacggagatg tttcttcgtt
     9841 atctttgcgt ataaagatga ttgtttttga taagatttta tctaaaatga taattactga
     9901 ataattttat taagtaattt tggttaaata ggttaaatag tttgattaag cattttgata
     9961 aataattttg gttaaataat tgtgattaaa aaattttgtt taaataataa ctaacttttt
    10021 atttatttag agggggggta gtaaatatgt tatggtataa ttccactgca gatgaaataa
    10081 aggaggctaa tcccgacata gcagtattgc ctgtaggttc taccgaacaa catggacctc
    10141 atcttcctgt gggtacagac ttcatgatag caggagcggt tgcaaagggt gttgcggaaa
    10201 aattaggagc ttatctttta cccactcttc caatcagcaa ttgccgtgag catatgggca
    10261 aaaaagggag catatggatg aacccggata ctttctatta tgtgattaag gatatacttc
    10321 ttagccttaa agaacaggga ttcaggaaaa ttgtatatat tcaggctcat gggggtatct
    10381 ttacaggtgc accggctata agggaagtta atgcaacaaa ccctgatatt aaagttgtaa
    10441 agattgacct cgtccaatat tttacttcag atgaaatgaa ggatattctt gagtgcagag
    10501 ataaccttca tgcttgtgag tttgaaactt cattaatgtt atatctgcag gaagagcttg
    10561 ttaaaaagga cttgattgta gattgcgttc ctgatgtacc aagagatttt ttaaattacg
    10621 gatctatatt aaaatattca aagagcgggg tttggggcaa accaagtctt gcaacaaagg
    10681 aaaaaggtga gaaactactc aaactattag ttgacaaaag cgtggaatat tcaaactggg
    10741 tttttaaaac atttgattta tgaaaaagtt aaccaagcat gggtgcattt attaaatatt
    10801 tcttcgcaat tactttataa ttaataagta aacccggcaa acataacaat taataaacca
    10861 ggtaaacata gaattaataa ataaatcgtg taattgaaaa aaataatttt aaacaggagg
    10921 attgaaatga ataagaaggt tagagtttta ttagtaggag cggcatttag tgccgacctt
    10981 catatggacg gttactccag gtgcagagaa ctggcagata tagtcgcaat atgcgataaa
    11041 gatctttcaa gagtaaaagc ccttgctgaa agatatggtg ttacagatta tacagcatat
    11101 gatgactaca aaaaagctat tgacgaggta gattgtgatt tggtagatat ttgtctgcct
    11161 aatttcctgc atcatgatgt agcaatttat gcattccaaa aaggacggca tgtaatatct
    11221 gaaaagcctc tcgctacaac cttagaagac gctgaagcaa tggttgaagc tgcaaaagcc
    11281 gccgacagaa aactatatta cgctgaagac tgggtaatgg cacctgctat gaacaaagcc
    11341 atggaaataa taaatgaggg agctatcgga aaacccttgt atattcgtgc aagagaatgc
    11401 cacagcggct cacattcacc ctttacccag acaattaaat attgtggcgg aggatgcatg
    11461 gtgcatcttg ggattcaccc aatagcattt atgctaggcc ttaaagataa taaatggact
    11521 gagcttgtag caatgacttc cggtggtgga gagaacaacc ttattcacag aacaatggaa
    11581 ggggaggact ggtcagcctg tatgatgaag tttgaagacg gaacagctgc tttactggaa
    11641 gcaaactatg taacaaccgg cggaatggaa gatgtaattg atatttatgg ggaaaaaggc
    11701 tgcctgcata tagatttgac tttttcatct ccaattaaat gtttctcaat acctggtgta
    11761 agttacactg ttgaaaaagc tgaagtaact acaggatggt cgaaacctgc agttgatgaa
    11821 aaatttaatt taggttatgt aaatgagatt aggcatttta tggagtgctg cctcaagaac
    11881 gaggatgcaa tggttggctt aagaggggtt gatggccttg aagctttaag ggttgtaaac
    11941 ctgatatata aatcagctaa ggaaggaatt agtataacta ataaaaattt ataacattaa
    12001 taacttggga ggtcaaatta tattatgaga aaagatttat atttgcctgt tgaagtggga
    12061 ggaataacat ttaaaaaccc tttttatgta gcctcaggtc cgaccactaa gtcggtaaag
    12121 cagctaaaga ggattgaaga aacaggttgg gctgctgcca gcatcaagct tacaatagac
    12181 ccggctcctt atattaacag agcaccaaga tatgccatgt ttaatgatcg gaatgcactg
    12241 gcatttacag cagagaaaag actgacattt gcagaaggct taaaattaat ggaagatgcc
    12301 aaaaaggaat tgaaggagct tatccttatg gccaatatta cctatgcagg agataaaggt
    12361 gttgagggct gggttaatat ggctaaaaag tttgaggaag ctggagcaga tattgttgaa
    12421 ttgaatatgt gctgccctaa tatgtcttat aatgttgaac tatcttcagg tgacagcaaa
    12481 tcctgttcaa ttcgtacagg agcaagcctt ggacagcagg gtgatgtagt tgcggagata
    12541 gtcagagaaa ttaaaaaagc aataaaaata cctttgtttg ttaaattgac tcctgaggga
    12601 ggaaaaattg cccgtgttgc aaaatccctc tatgaagcag gcgctgattc agtaggcgga
    12661 acagcaaacc gtttgggaat accgccaata gatcttgata atccggggaa agcagtatac
    12721 catctgcagg atgaaataag catgtcctgc catgcaggtg gatgggttaa accattggct
    12781 ctgagagata cttatgaaat aagaaaagta aacggaccgg accatagtat tatggctgca
    12841 ggtggaatca gaaactacag agacgctgct gaaatggtta tgtgcggtgc tgatttgctg
    12901 ggtatatgtg cagaaactct tataagcggt tttggattta ttgaaaatgt tatatccgat
    12961 cttaaaaatt atatggagga acatggatat aagagtctga gagaaatgcg tgatacaatt
    13021 gtgcctcttg taaaaactgc tcctgaactt actatttaca agggtaatgc taaaattacc
    13081 gagcctaact tggcagcgcc atgtaaagct gcatgtcctc ataatgttcc tgctcaggca
    13141 tatgtacaga aagttgcaaa aggtgacttc aaacgtgctt atgagcttat aatgtcaaag
    13201 aatcctttac agtctgtatg cgcatgggta tgtaatcatc cttgtgaaga agcatgcacc
    13261 cgtggcgaga ttggtactcc aataccaata agagaaatta aaagatttgt tatcgagtat
    13321 gcaaagaatg caggctggaa gcctgaaatc aataaaaaag cagaaagaaa tgaaaagatt
    13381 gctgtaatag ggtcaggtcc ttccggactc tcctgcgcat ataatcttgc tttagcaggc
    13441 tataaagtaa ctgtatttga aaaggagaat tatcttggag gaatgcttcg ttatggtctg
    13501 ccaagattca gaatgaacca cgaagttctg gataatgaaa taaatttcct gaaagatgtg
    13561 ggaatcgaat ttgtaacggg aaaaggtctg ggaagagata ttactgttga ttctttaaaa
    13621 gctgaaggtt attccgcaat atatgtagga ataggtgctc aggaaggaca aagccttaat
    13681 atttcgggtg aagatgctaa agggatatat ccggccatat ctttccttaa atcagtttac
    13741 gatggaaaag ctccaaatat aggcagaaaa gttgttgtta tcggcggtgg atttactgct
    13801 gttgactctg cccgtactgc tagaagactg ggtgcagatg aagtttatat agcatataga
    13861 agaacaagag atgaaatgcc tgcttcagca caagaaattg cagaagctga agaagaagga
    13921 atcaaggtta tgtaccttgt tgcgcctaaa gaagttgtag ttgaaaatgg taaagttgca
    13981 ggaatcaaaa tggtaaatca ggttctggga gaaaaggatg attccagcag aagaagacct
    14041 gaagctgtgg ctgcagcaga gtttactctt ccatgtgata ctgtcatagc tgcaacagga
    14101 caaaaacctg aacagtcagc agtaaaaggc ttcactgtag ataagaaagg tatgattgta
    14161 agtatgcttt ctacaggagc aaccagtatt gaaggcgttt tcgccggtgg tgatgtagtt
    14221 aatgttgaaa gtgtaattgc agctatttca gcaggaaaat gcggagcagt atcaatagat
    14281 aagtacctgg caaaagaagc tgcaaccctt gaatacgagc ctgaataccc agttgtttca
    14341 aaagaagccg ttttaaagag atcaggatac tttaaagata atggtccaat tgacataaac
    14401 actatagatg gaaaagaaag aataaagagt tttacaactt atacacgtgc attgacagaa
    14461 gaggaagcag ttgcagaggc caaacgctgc ctcaactgcg gctgcggaga aggctgcggc
    14521 ttatgcgcat ctatatgcag cgagtttgca attcatttga aatctgatga ctgctgggaa
    14581 attaacaaag atgagtgtgt agcctgcggt atgtgttata accgctgccc gaacaagaat
    14641 attgagatga ttaatcataa tgttctggtg aagtaaattt aaataaataa gacataataa
    14701 tcaatggggg cacgtgcccc gttgaaatta gacaaccaaa aggtgacatc cctcaatgtg
    14761 aaaggaatac ctcctgtcga gagaggagtg tcaaaattga caatcagaag gggacattcc
    14821 tcaacgtgaa agaaatacct cctgctcgag agaggagtgt ccccttacat tagaaaggag
    14881 tttcttatga aaaaagtact tattactcct agatcctttc cccagtccgg taaagaagca
    14941 tataagcttc tcagggaaaa tggatttgag ataatagata atacctccgg gaaaagcttt
    15001 gacgaagatc aaatggttga gcaatgtgct aatgtggatg gagtaattgt tggtgtagac
    15061 cctgttactg aaaatgttct aaaaaatgct aaaaaactaa aagcaatatc aaagtacgga
    15121 gccggattag ataatgtaga cttgaaaacc gctgaagaac ttgggataaa ggtagaccgg
    15181 gcagcaggca caaacgctgt ttccgttgca gagctcgctg tagggctttt ttttacatta
    15241 gcaaggaata ttttatactc tgctaaaagt gtcaaaagcg gaggttggga taggcaaaga
    15301 ggtgttgagt taaccggcaa aacagtagga attcttggac ttggtaatat aggcagagaa
    15361 gtagcccgaa tggcttccgg tctgggtatg aatgtaatag gttatgatcc atatgtaaat
    15421 aaggaagacg aaagtgttaa aaaatattca ataaaactag ttgataaggc tgatgtaata
    15481 aaaaatgctg attttttaac gcttcatatg ccgttaaccg atgaaacaaa gtatatcatt
    15541 aatagtgaga gcctcaatac aatgaaaccc ggagcttttc ttgtaaatac ggcaagaggc
    15601 gagcttgtag acgaagatgc tctttatgat gcgctggtat caggcaagat agcaggggct
    15661 gcagaggatg ttttctctaa agaacctcct ggagaacaca agctgctggg attggacaat
    15721 ttcatacttt tatctcatat tggagcattc actaaagagg ccaatgaaaa gatggctata
    15781 aaatctgctg agaatttgat aaggatgtta agtgaggcct gatattatat attttacgat
    15841 ttgaaagagt taatgaagaa tttacagaac gtaagttaca ttaaggtaac agaagcaggt
    15901 ttttcatttg tataggtatt tgagaaacac tatactagag cttaaccaat ggttaaataa
    15961 agtataaata gttattatct gggagaaatt gatatggatt tatatatggg agttgatatt
    16021 ggaacatcag gagtaagggc ggcacttttc gacaagaatg gtaaacagct tggattgcat
    16081 cacgaagagt atcctatgat atgttcagaa cctggtatgg gcgaactgga ccctgaaaga
    16141 gttttcaatt cgacaataaa tgtagtaaag ttatgtattg aaatatcggg cgctgatagt
    16201 aaaaatattg cagctatagg cttcagtaca cagttattca gcatacttgc cgttgattct
    16261 gaaggcaaat gcctgacaaa tgttattaca tgggcagata aaagatctat agctcatgct
    16321 gacttaataa aagataaaca tgattatgta aagatgtata acagcaccgg ctgcagggct
    16381 cagcacccta tgtatcctgt aagcaaggtg ctatggctga aagatacaat gcccgaaata
    16441 ttcgcaaaaa cacataagct tgtttctata aaggaatatg tgctttttaa actttttgga
    16501 aaatatgtaa ttgatattac cgacgcctca acaacaggat attttaacat tcatgatttc
    16561 acatgggata aatatataat agatgatgtg cttggaatat ctccggatat tctgggtgaa
    16621 gccgtggact gtacttttgt actgaaaaac atgaaaaagg aatatgctga aaagatgggt
    16681 ataagtcctg aaactcctgt tgtaataggg tctggagatg gaatgcttgc aaatgttgga
    16741 tgcggagttt ttgacaatac atcaatgtca tctacaatag gtacaagcgg agctcttcgt
    16801 atagccacag aaaaacctat attggaccct ttacaaagaa catggtgtta ctgctttacc
    16861 agagatacat gggtagccgg cggcgctatt aataatggcg gaattgtatt aaaatggctt
    16921 agagaccagc atagggcagc ttatgaagcg gaagcaagag aagctgatat ggatgatgta
    16981 tatcagttgt ttgattctta tgctatggaa attaatccag gaagtgatgg ccttattttc
    17041 ctgccattcc ttacaggaga gagatcgcct aactggaatg catcaacaaa gggtactatt
    17101 catggaatgc agctcataca taataagaag catttagtaa gggctgctat ggaagctgta
    17161 atgtacagga tgttttctgt atatgaaatt ctaacagcat tcagtgataa tgttaaactg
    17221 gtgcttgcaa acggtgggta cgcaaattca gatatatggc tgcaaattca ggcggatgta
    17281 tttaacaggg aaatagcagt agcaggcatt ggagaagcct ctgcttttgg agcatcatat
    17341 gtagctatgg cagcaacagg tgctataagt tctctaagac agccattacc tgctatgcag
    17401 ccgaagaaag caattaagcc tataagagaa aatagcgaaa tatataaaaa tgcctataaa
    17461 aattttaagg atttgtatgg caggatttat acgaaatagt ataatagaaa taatataaag
    17521 tatgaggagg catatagttt gaaagaaatg gaacttaaaa tgaacgaaat attaaataag
    17581 atcggagatt atggaatagt ccctgtcgta aagattgatg atgctaaaga tgcagttcct
    17641 ctaggaaagg cacttgttga tggtgggttg cctgttgccg agataacctt tcgcacaact
    17701 gctgcagaag aagcaataaa gaatataagc cgggaattac cagaactttt agttggagca
    17761 ggaactgttc taagcgttga acaggtaaag aaggctgtta atgccggtgc aaaattcatt
    17821 gtcagccctg gtttcaatcc ggatgtagtt aaatactgcg ttgacaataa cataccaata
    17881 acccctggat gcagtaatcc tacagacatt gaaatggctt taagctttgg gttggaagta
    17941 gttaaattct tccctgcaga atcttttggg ggaattagta cattaaaggc cataagtgcg
    18001 ccatacggca tggttaagtt tatacctaca ggaggaatca atgccgccaa tattaatgat
    18061 tacctttcat ttggaaaagt tctggcgtgc ggtgggagct ggatggtaaa agacgaactg
    18121 ataaagaaag gaaacttcca ggaaattaca agattaaccc gcgaagctgt aaataccatg
    18181 ctgggctttg aaattgctca tataggcatt aattcagctg atgcagcaag ctataatgac
    18241 gcatcggaaa aactggcggc agcatttaat ttccccagga aagaaggaga agtatcaagt
    18301 tttatcggga ctgctttcga agttatgaaa agtataggaa gaggtaaaaa cgggcatatt
    18361 gctataaaaa caaacagcgt gtccagagct atggcatatc ttacacgcaa tggtgtggcg
    18421 gttgacatgg attcagtgaa aaattccccg gcaggtcagc cggtatttgt ataccttgcg
    18481 gatgaagtag gaggatttgc aatacatctt actcagaagt gagcaaacgg ggagcaagtg
    18541 agaacgggat tcttgcatgc aagaaaataa gtatggttct ttcatgccag caaatgagta
    18601 tggttcttgc atgtctaagg taaataggga cagcttaaag gctcaacggg cagatattta
    18661 ctgcttgtta agcctttatt tcgtatcata ggaagaaaat gagtgcctgt ttaaaatgaa
    18721 aactacccta aaaacaaaaa ctatgttaaa ataatattag agtaaaaaaa ttttagctta
    18781 ctgctacgcc aatttggaaa gcgatgtctt ccaggataat ttttgacagc atcagtaaga
    18841 tgtcgcaggg gaaatgtctc tatagaaggc cttgcgaccc taccctcaga tgtattcctt
    18901 acgggttagc aaaaattacc ctccagaaat gtgcttccca ttggcaacag aaaaaagtag
    18961 tattactcta atattaaaat ttattttaat cgggtgagaa ctgtgggcta taactatatc
    19021 gatggcaata tgtatgaggg tgttcatata gtaagaatag aggataacaa aaataatctg
    19081 ccttttttta tcataaagat gggtgtatgc aatcagacca agcatatgca tgaatttgtc
    19141 cagattgtat atatgtgtaa agggaaattg aagcatgtaa taaataataa tactttcgat
    19201 gtttataaag gtgacatatt tattattcca ccttatgtac ctcactattt tattgctcca
    19261 tatgatgata aatttgaact cattgagttt gaatttatac cagaattcat taatgagaaa
    19321 ttctcagttc ttcaaccagg tgcaaattca aacaactcaa aagtaataga cagtgctttt
    19381 atggattttg cgtaccttga accgttttta gtgtcggagc atgaagtaaa acccaggctg
    19441 aatcttaccg gaagcgtaca aattgaggtg gagaaaatac taaatgaagt aatactggaa
    19501 tatgagcaca gagatattga ctttgagctt ataataaaag cattgctttt aaaattatta
    19561 attattgtcg ggcgtgagta taagaagtca acctctggta ctgagtttga tgatatcctg
    19621 gcaaggcata gagatgctct ttatgaagcc ttggaatata ttaataaaaa ttttacaaaa
    19681 gaaataacca ttgatgaggc tgcaaaagta gctatgttgt ctcagtccta tttcagatac
    19741 cttttcaaac aaatgacaca gaaaacattt actgaatata ttaatggatt gagaattgca
    19801 aaagctgctg agctgcttaa aaacaattat gaaatgaaag tggttgatat ttgctttgaa
    19861 gttgggttta atagtgtaaa ccactttaat cggattttca gagcagaaac aggttttaca
    19921 ccaatgcagg taagaaaagg gaaaatgaac actgggcagt gatattgcta tgttgccaat
    19981 ggaaagcgca gttctgaatg gtaatttttg cttacctgtg ctgcaggaat ggagcttaat
    20041 ataaagtaat catatcagtt caacctataa tggttaaaat agttatttca aattatgaaa
    20101 ggggatattt ctatgaaaaa gccaattatt atagctcata ggggggcttc tgcctatgca
    20161 ccggaaaaca ctttgtcatc atttcaaaag gcactggaaa tgaaagctgg aggtatcgaa
    20221 atcgatgtcc acttaacaaa ggatggatac ccggttgtgg ttcacgatga aaagctgggt
    20281 cgcacatgta atggaacggg ttttattaaa gaaaaaacac ttgaggaact taaggaactt
    20341 gattttggca gctggttttc tcctgaattt gcaaatgaaa aaatacctac tctcaaagaa
    20401 acaatggatc ttattgcaga taaaaatata cttttcaata ttgagataaa aaatggccct
    20461 atattttatc caggaatcga aaaggttgtt gtagacatgg ttaggatgta taatatgtct
    20521 gaaaatgtaa ttgtatcttc atttaatcat tacagcttgg cagaagtgaa aaacattgca
    20581 ccggaaatta aaatagggct attatattca gctggcttgt ataacccgtg ggaatatgca
    20641 aagatggtgg gagctgaagc aatacatcct ttctacatga gcatacaccc tgaagcagta
    20701 aaaatgtgca atgataatgg tatattggtt aacccctata ccataaataa tcctgagcat
    20761 atgcaacttg ccttacttgc aggtgttgat ggaattatta caaattatcc ggatatagct
    20821 ttaaacatag ttgaaggggt tgggtgattt tatgaagctg gactatagga aaacctttgt
    20881 aataggactt ggctttttta ctgttagtat tatttggtct atatacaatg tagcagtacc
    20941 acgctatttg gataaccttg gactatcagg tattatggtt ggtacagtta tgacaattga
    21001 caatttattt gcacttatat ttcttcctgc attcggtaca ttaagtgata ggacaaatac
    21061 caggtatggg cgtcgtatgc cttaccttct tgtagggata cctttatctg caattgcatt
    21121 ttttcttata ccattttcca gagaaagcct tccatttttg atgacgacgg taatactcct
    21181 gaattttttt atgagcatat atagggcgcc aacagttgcc ttaatgccgg atttcactcc
    21241 tcagcctttg agaagcaagg caaacggcat tatcaatttt atgggtggat taggggcatc
    21301 tgtggcattt ttaatgggtg gtgccctgtt taaactgaaa gagtattacc cttatgtggt
    21361 tggatcttcc atacttattc ttactatcat actaatgtat aattttatac aggaaccaaa
    21421 agtagtagac gagattatgg aaaaagataa agaaaaggat acacataaag atacatataa
    21481 tgataaatat gagggcacaa atgaatatga tacttttaat agtagggaac agggaaatgc
    21541 acttctggat caaaaagaag caaagaaaga aagcggagaa cgcctaagct tggtattcct
    21601 attgctggcc atatttttct ggttcagcag tttcaatgta gttgaaacgt ttttttctac
    21661 atatggagag aagatattaa atgttgataa aagtgtttca tcctatcttt tactcacagt
    21721 atctgcattg ttccttgtct tttcaatacc agcaggatta attgcgggta agctggggcg
    21781 caaaagaacg attctatacg ggcttattgt tataatcatt acctttatca gtttaatatt
    21841 tgtaaaaacc ataactttaa tgtatgtctt aatgggaata ggaggaatca gctgggcatt
    21901 aattaatata aattcatatc ctatggttgt tgaaatgaca tcaaacaaag gaataggaaa
    21961 atatactgga tattattact ttttctctat gctggcgtca attgtaagcc cgatcttatt
    22021 tggcggtatt aaggatatac tgggagatgg atttttgttt atatatagta ctatcggcat
    22081 gataatagca ttctttttca tgatgcttgt gaaacatgga gaagctaaag ttgttgtaaa
    22141 atcggcattt gaaaatcttg aacatatgga gtagaataag aatggaataa taaagagata
    22201 gacgggcaag ctgggatatt tccttgtgaa ataaaatatt cttaggagtg attttaatgg
    22261 atttaataaa aagtgcttct gaaataataa gtgcttctaa taatatcgtg gctttcacag
    22321 gagcaggtgt ttctactgaa agtaatatac cggacttcag atctgcaggt gggctatata
    22381 acagcaaaaa taaatttaac tatccgcctg agtatttact aagtcatacg ttttttgaag
    22441 aagaaccgga taattttttt aattactata gaaacaatct gatatatagg gatgcaaaac
    22501 ctaacaactg tcatatagct ttggcaaagc ttgaagaagc aggtaagctt aaagcagtta
    22561 taactcaaaa catagatggt cttcatcaaa ccgccggctc aaagaaagta cttgaattac
    22621 atggttctgt agatagaaat tactgtgcaa ggtgcggtaa aaaatatgat ctggatttca
    22681 taatggactt aactctgtca ataccaagat gtaaaatttg tggtggtatt gtaagacctg
    22741 atgttgtttt atatgaagaa tctcttaatg aaagagtgct ggataattca ttatcattta
    22801 ttgcagcagc agatacaatg attgttatgg gtacgagtct tgttgtttat cctgctgccg
    22861 gtctcctaaa ttattataaa ggagataagc ttattttaat aaataaaaca tcaacccaat
    22921 acgattatag agcgaaaata gtcataaacg acagtgcagg cagtgtcatg aaaaagatcc
    22981 tggagaatat aagattataa gcatggataa ttagtagaca taagtgctca tatgcatgtg
    23041 ggtgcatatt ttcttcactt aaattatata caccctatga atgttttcgc gtagtagttt
    23101 tgggaaaata aattacacgt gcagctgaaa tgaatttaag tccataaaaa ctttttcata
    23161 aaaaagtttt attcctgcaa gggcgaattc atttagcatg cagcaggaat ggagcttaaa
    23221 gtgttaagtt gggaagaatt gattatagaa tgctcaaatt gtatgaaatg cgagcttggc
    23281 aaacatagaa ctaatattgt tgtggggaga ggaagtagaa atgcaccttt gatgttgatt
    23341 ggggaaggcc ctggacagca ggaggataaa cagggccttc cttttgtggg ggctgcgggt
    23401 aaacttcttg atctactgct ggatgcccag gaaataactg aggataaata ttatatttgc
    23461 aacatagtaa aatgccgtcc accaaataac cgagttcctt ttgatgaaga agcattccaa
    23521 tgcttgcctt atttacgcaa tcaggtaaag cttgtaaa
//
LOCUS       DAOSDD010000040        23531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_966_length_23531_cov_5.198841, whole genome shotgun sequence.
ACCESSION   DAOSDD010000040 DAOSDD010000000
VERSION     DAOSDD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23531
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_23531_cov_5.198841"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK733_05485"
     CDS             complement(<1..266)
                     /locus_tag="PK733_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10029.1"
                     /translation="MYNFSMLLDDNTCTNLEGKIKVFNKFHINNIEINDCIDGKKITE
                     LNGEEIEKIRNVLIQYNKKIVLLSCNTDVSYYEYYKTVFRQAHTL"
     gene            complement(297..1196)
                     /locus_tag="PK733_05490"
     CDS             complement(297..1196)
                     /locus_tag="PK733_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ10030.1"
                     /translation="MANIGNMLSSSNINVINDIINKEDIKFSLELMGIVKGDVLIVHS
                     SLSSIGYVDGGADSVIDALLETVGDEGTIVMSTLTGWFDHFDADTSPSAVGKISEVFR
                     KRNNAYRSLHPVHSVAAIGKHAKYITDGHNNCETGCGQGTPYYKIKELNGKIMLLGVD
                     MDRNTIMHCLEEEIDAHYLRTLDIVAPTYIENYKEKIFILKKFPPGHRDFLRITPELR
                     KKDALIEGKIGNAIVKVIDMEKLFNMGLEILKNDPLFFICSNPNCNSCDWSRLLYSDT
                     DIDYTRYRGNGCVDNTCEICVVD"
     gene            complement(1267..2034)
                     /locus_tag="PK733_05495"
     CDS             complement(1267..2034)
                     /locus_tag="PK733_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOJ10031.1"
                     /translation="MELNMRVIDITGPIYEGMWNYEEPFPKFRMEPLGEVEWAKAEVF
                     CEIFKGMHSQTGTYLETPAHFYGNDKCYLVADIPIEKLVNIPCYVINLDEKDFNVPNI
                     YPGISERKSAHIGMEIKTINTDVERKYINADMMKAACYEGIDIPVNSAILVGTGWGKY
                     WREKFYLDKSPYFTYEAMEWLVSKKPFLLGTDFPRWENLDKPEGFFKDFYAADILMLA
                     PCINLEKITRQQVKLTALPLNIPGTSSIPCRAVVIED"
     gene            2190..3389
                     /locus_tag="PK733_05500"
     CDS             2190..3389
                     /locus_tag="PK733_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ10032.1"
                     /translation="MNDIKRFHKDMILLFLAELLWPLGLALYSSFFPIHIKELGGSDI
                     VVGIIMSIPPLMGVLAIVGGIWADFDDRRNVIIFGWAITIPAPLIWAFATSWQWMLIG
                     QIIYALTWVCSPAITLYILDYDTTGNKIASYTMVYLAAPIGSILAPAIGGKIASSYGR
                     QTLYLIVFILYSVSTLCTLLLSKQPVQNKEKNVDECYTRFSIKNKLSDTRDSIKQLAG
                     IITFLTILVSSQNIGESFLSLYMNEFRNISVENIGLSFTALFAGASLFTFLFGKSDKK
                     ISSGTALNTGNIIFIISALLVCFTQNSISLLVLAFFLRGINRSIVVFTQVVLAKNINN
                     ENNKGFILSLYIAVRSILIGLVTYPGAALYRINPAYPFYAEALLVIIWMAVSYNSHLR
                     NRFINYE"
     gene            complement(3399..3563)
                     /locus_tag="PK733_05505"
     CDS             complement(3399..3563)
                     /locus_tag="PK733_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10033.1"
                     /translation="MNYFVILILGLVSFYLFTFARYNWKVKNKRAAFGLIILGLITFI
                     YPAVMIVLRW"
     gene            complement(3566..4888)
                     /locus_tag="PK733_05510"
     CDS             complement(3566..4888)
                     /locus_tag="PK733_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ10034.1"
                     /translation="MKSLKVGRYANTSLIQVLKAFIVSLLLIFILAGCYDAKEIDELT
                     YVLAIGVDKGVADTYRLTVKFHTMKEAGGGGAGGKGGGGGGMAGGEHEIITIDALSFF
                     TGINMINAAVPRKLNFMHAKFLVFSEEIAKTGVGEFIAPIIRFREIRRTLHVIVARGK
                     ASDFLAEYEPVVGTTLSKAMELVVAEPANIGFFAHITLNDFYNGLKSTYRQPTAILGG
                     INKFENFREDGPTVPDTGNMKKKEYYAGEIPRKGGSKIEFYGTAVFNGDKMVGELTGE
                     ETRLMNILTGKFRRGFFTIEDPKAPPLIVPLDVREQRAPDIKVKLIDGKPYINVVIRL
                     EGDILAVQSRLNYEEDPLKTLLQDSFIKQIKNEMNTLIERCQKMDSDIFRFGRIVSSK
                     FYTLQEWQNFNWIKKFKDAEVNIDMDFAIRRTGTLLKSSPILTTEEAK"
     gene            complement(4947..6077)
                     /locus_tag="PK733_05515"
     CDS             complement(4947..6077)
                     /locus_tag="PK733_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOJ10035.1"
                     /translation="MVKEGKFGPQEAISLITITIVIRVFFTSPSITTRFVGSAGWYMT
                     LISAATAAAGFTIIYLLIKKFPDKDLIEIYEITLGPYIGSLFSFAVFLIFIIDAGIVL
                     REFTEVMKVYVLPLSPPSYIIGIFVLGVIVLGILGLETQARFARLIAPVLLISFIAGL
                     ILAAPNYQVHRIKPVFGYGLGNVVIHGLFRSSVYGYAIIAVIIAKSLQGTKYVKMVGY
                     TSIILSGILISSALLAFSLTFPYYIAQEVTSPLYQMTALIDFGRFFQRLDPLFLFIWN
                     ISTLIAVSIQLYISASIYSKIFRIQDIGPIIIPLSIIAFATAMMPKDLESIALGYVNT
                     LREYAWLVYYVPAFIALIAAWLRGKEKRSRRGEGRKRMKKSV"
     gene            complement(6111..8090)
                     /locus_tag="PK733_05520"
     CDS             complement(6111..8090)
                     /locus_tag="PK733_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ10036.1"
                     /translation="MSNTSNTIKFLRNLITYNGPKDVEGFELLETEYEGTERDGYDEK
                     INDQKRKTAEQESKNSENEDKKKNIRQGTQKNQQENVQQSNQESKNSEKTQDKRETDD
                     VKKSKKPLNVDEWNQAKNKEDKVGEKEPTFEESGSSGKDEGKGEGRGEEDSQSTANIK
                     KVSPKLYTNLKKIQDEFISNKNSDIIIRQFKIGKQINAFITFIDGMADRETINNFILR
                     QLMLPELFQGLSNEECSIEYIEDNVLAVHDTKREKNYEMIIRQILNGLTALFVDGCNE
                     CILFETRGYEKRGVERPSTENVIRGSQEGFNENLRTNITLIRRIIKNSHLVTEVLPVG
                     RENNSHCGILYIDGITNPRIVNEVKRRIKSLDIDYVHGDGMIEQLIEDDSSSIFPQAL
                     STERPDRAASFLIDGKVVIIVDGTPFALAVPINFYNMLRTSEESNMKWQYGTVIRIIR
                     VFGLLVSVLLPGAYIALVLYHQEMIPTDLLTAIAKSRENVPFPVVVETILMELSFELI
                     REAGLRVPGIIGTTLGIIGALILGQAAVAANIVSPILIIVVAVTGLGSFALPNFSLGI
                     STRILRFFLIFFGAIAGFYGISVGIVIIGGFLCSIKSFGVPFVSPVAPKTMSDAELIL
                     KKPMYEPAERPDALNTLKRRKASANPRKWGDNKNK"
     gene            complement(8288..9172)
                     /locus_tag="PK733_05525"
     CDS             complement(8288..9172)
                     /locus_tag="PK733_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOJ10037.1"
                     /translation="MKKNKDWLDIYKKTLYVISIVIILLIVVVFAIVSKQNFNIYTGD
                     IENNEDDFINYSVSSNYTKPKLAIIIDDFGQNRDGVKEMMEIGRPMTFAIMPFLTYTK
                     ADANKAHEKGFEVIVHLPMQSQDRDIASWLGPRPIKLGQSNNEIRRIVLDSFRVVPHA
                     VGANIHMGTKSSEDPRVMSCVMKNMEEKNLYFVDSMTSSKSVCREVAKDIKINFVERN
                     YFLEQSSIKTMAYARQQLLTAGELAVKKGYAVVIGHVGSAGGRTTARAIKETIPQLEK
                     MGIQLVQVSKLFRQLSGK"
     gene            complement(9507..10733)
                     /locus_tag="PK733_05530"
     CDS             complement(9507..10733)
                     /locus_tag="PK733_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10038.1"
                     /translation="MAVNVSMYKYENRRGGKGGSIRNHSRAPNNNIYAKERRCSDAKS
                     ILTFKVAATYIGTVVGAGFASGQEILQFFSVFGIKGFWGLVLVTVLFVIYGHIIMELG
                     MYLGSTSHLKIIRYAGGKYLGPVIDFLIIFFLFGSLTAMIAGSGAIFRQQFNIPAVWG
                     NLVMAVITAITVLTGIEGVINSISFIVPFLIVAALGVGIASIFFVHPDYTHIHKVSGN
                     GLIGSWLWAAILYASYNIILSVSVLAPLGNNIQNNESIKKGALLGGVSLGATGAAIYF
                     TLVRNLGSIIHMEVPMAFIAGEISHVVQVGYIIVLLAEIYTTAVGSLYGFTARIINSD
                     SLMNSIGSSRLASLTGKTCIVFTAFLAFITSRFGFSNLVSYLYPVMGYGGVILLVSLL
                     YCKITKKVTGKKNRIQ"
     gene            11218..11913
                     /locus_tag="PK733_05535"
     CDS             11218..11913
                     /locus_tag="PK733_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10039.1"
                     /translation="MDKDKILIVDDEDGIREMLREYLSPEGYIIDEASDGIEALELFR
                     KNKYSLVILDIMLPKMDGWIVLREIRKMSQVPVIMLSARGEEYDKLFGFELGVDDYIV
                     KPFSPKELLARMKVIIRRSTNPALSESKENSVKYEGLEIDFDSRNVTVDGTSVNLTPK
                     EYELLNFFTMNPNKVFSREQLLNEVWGYDFYGDNRTVDTHVKMLRESLGGYRKFIITV
                     WGTGYKFETGVRK"
     gene            11910..13361
                     /locus_tag="PK733_05540"
     CDS             11910..13361
                     /locus_tag="PK733_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ10040.1"
                     /translation="MRSIAVKLWTAMMLLIIIMLALLWFFQIVFLENFYINQRVEEVK
                     NRSILIIQDINVTGKEELETRLESLIYDYNCNIDLIDTAGKIIYSNAAGRQMPMMGAG
                     HIKKELMSEVLSGKTVTTSLIHPRFNSKYMLIGIPVKSSTGEVKEITEITGALLINMP
                     LAPVADTAGILKKQLVYICFVLFSAALLLSFLLSRSFIKPILKITEAADKMASGNFNV
                     RISSESKDEIGKLSRAINNLGEELSKIEQLRRDLIANVSHELRTPLSLIRGYAETIRD
                     VTGNNKEKREKQIGIIIDESERLNRIVDDILNLSQIQSGNVVLNTTNFNLNRTIRNVI
                     GKYEILSNQSGISVVYATNIGDRNNEVPAANANIMVKGDEARIEQVLYNLINNAFSYS
                     KSGDSITVKTLVGNGIVKVEISDMGEGISPENIKHIWDRYYKSDKSVTRKQIGTGLGL
                     AIVKSILEVHKSRYGVESALGNGTTFWFELDLS"
     gene            13471..14037
                     /locus_tag="PK733_05545"
     CDS             13471..14037
                     /locus_tag="PK733_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HOJ10041.1"
                     /translation="MKNMKKFAAVLAVVAIIATAGAVFAADLKTPVEIVSGLTGKSVE
                     SLSQERAAGKTYGAIAMESGKLDEFKVQILEQKKALLDQKVKGGTLTQEKADEIYNAI
                     KENIANCDGSGATCIGRKYGVGFGSGSGACAGNGICNGTGAGNSTSTGIGASNGLAGG
                     MRRGKSAGMGMKRSLQGQGQGRGMGLGR"
     gene            complement(14187..15473)
                     /locus_tag="PK733_05550"
     CDS             complement(14187..15473)
                     /locus_tag="PK733_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HOJ10042.1"
                     /translation="MTAVKEFIKIENLTKKFADSEIPVFKDVNITINNNEYVSILGPS
                     GSGKSTLLRVITGLMEPTSGRVIFEGKEVKGSNQGAAIVFQSAALYPWLTVIENVELG
                     LKAKGFPKDYTEEKAEKLLDLIGLDGFEDAYPKELSGGMRQRVGFARALAVEPKLLCL
                     DEPFSALDYLTAENLRTELVELWQEKRMPIKGVIHITHNIEEAVLMSDRAIILSKSPA
                     TVLAEIPIRLPHPRNRKSKEFEMMVDHIYKVMTRKEIESTNNKTKNQISDTGEEKLKN
                     LPHAHPGVLAGLLEILEDNDGKIDIYKLGRDLLIESDELLPSIEAIALLGFAEVREGD
                     AIILPLGQEFVDSDMDGRKKIFRERIKQLPVFKVVLNVLNNKKDLSMKEEFFVDLFLH
                     HFTPQEVDILMETITSWGRYSELFNYDYDSEVIYIE"
     gene            complement(15511..17256)
                     /locus_tag="PK733_05555"
     CDS             complement(15511..17256)
                     /locus_tag="PK733_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10043.1"
                     /translation="MKKEKNLMFHSITPADGVVIAVIIAVMYLLISLNIDNPIYKSNI
                     VISLNTKYLPAYALFSFLRMLAAYILSLVFTLIYGYVASQNKKAEKIMLPLLDILQSI
                     PVLSFLPAVVLGLVAIFPHSSLGVELSSIILIFTGQVWNMTFSFYHSLTTIPRDLDEV
                     ASTFKLNKWQKFKKLQLPFSAIGLVWNSMMSWAGGWFFLMACEMFTLKDNDFRLPGLG
                     SYLQTAAFQGDTKALLHGLITMIILIVLIDQLLWRPIIAWSDKFKVELTGGGDAPESV
                     VLYIIRRSVLIETLSNKVTRPMIRYIDEFLNKLFSPRGTPEYEIGMNQCSKARRDALK
                     KWVYNVLGIAAILLFAYGAFRAAEMLLKLSGGELTQLFPSALASLVRVIVAQIIALLW
                     TVPIGVAVGLNKKLANILQPIIQIVASIPATALFPVLLIALLKFAGGLNIASVALMLL
                     GTQWYILFNVIAGAMSIPEDLKEAAANLGLKGFKKWKTLILPAIFPSILTGLVTATGG
                     SWNATIVSEYIGFGGEHMKTLGLGALIAQSTDIGNYPMLLASTLVMIIIVVGTNRFIW
                     KKLFRFSEERFRLDI"
     regulatory      complement(17463..17627)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(17830..18528)
                     /gene="ytaF"
                     /locus_tag="PK733_05560"
     CDS             complement(17830..18528)
                     /gene="ytaF"
                     /locus_tag="PK733_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HOJ10044.1"
                     /translation="MVSTICILALSLSLDALGVGIAYGLRKIKIPVLSAVFICMFSIL
                     YSGASVYFGKYISSIIPPGISKLIGIFILLLMGCWIIIHGLIENKSMDISKSDVDIKA
                     NKYIKNNLNILDIKDETLIKVAIKSLGITIQVIRNPSECDIDKSGTIDVKESILLGLA
                     LSLDAIGVGIGSALSGYYSAFLPLAVGLFQLVLLFVGTFLGKRITAMEKFNQKIITIV
                     PGILLIILAIIRIY"
     gene            18761..19639
                     /locus_tag="PK733_05565"
     CDS             18761..19639
                     /locus_tag="PK733_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ10045.1"
                     /translation="MVFIVTALMIEAAPIIKHFKLKKDMSVHKYQVYKNSAIVLIISG
                     VGKVRSAMASVYLLSNHEITGTDIFLNIGFCGAGSTKYPPGSMLLINKITDIDTGKDY
                     YPDVFINQALPREAICCYSRPLHKEDLKSQTEIFCDMESSGIMEAANKFIYAHQVVIL
                     KVISDYLTPENLNKDQLQTLLENHMPYIEQIINELQQLNELLQLNEGLNDRCSKFTSK
                     EEKEVIAIISDISDNLRFSVTMKQILFKEAKKAKQKGIDPLKILKPLTETKVNSKVEG
                     KKIFGQLIEKLEQTLV"
     gene            19599..20621
                     /locus_tag="PK733_05570"
     CDS             19599..20621
                     /locus_tag="PK733_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HOJ10046.1"
                     /translation="MGSSLKSLNKRLFNTIYVEEAAYGYTITREMLQKYPDCPVIPIR
                     HYKDVFNRTNQHYAIQKQYPSLILAVKGNQFLYKGPAVCQDFGHSGHSAFYYTSFLLN
                     CIFNCEYCYLQGMYPSANIVAFVNVEDFKFAMEQAVMKEPVFLAISYDTDLIGFHNII
                     PYYDYFCDFFASHSELYVEIRTKSANQTFYQNHTPLENIIIAFTLSPEEVIQKYERHT
                     PALEMRIKAVQVAIKHGFKVRICFDPIFIHPELDNLYEPFFHYIFTQIDPGSIQDIGY
                     GFFRMSKHFFKRIEKHQINSQLFSEKYWVDKDVVSYSPELQEKIKSKHFGILTQYVKE
                     TSIVTI"
     gene            20708..21406
                     /locus_tag="PK733_05575"
     CDS             20708..21406
                     /locus_tag="PK733_05575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ10047.1"
                     /translation="MKVAILTGASKGIGLALTKRLISLNYKVYGISRSFQNTPFSHAN
                     FIRVECDIQDTGKLIQVIESIEDREANINLLVNNAGIGYFGPHEQIKIKEIQTMTRTN
                     LEAPLILCQLLLRALKKSNGTIISISSVTAKKVSTHGCAYAASKAGLTHFFTSLFEEV
                     RKSGVKVTTIHPDITKSSFYDSLDFTYEDESDTVLLDEQIADAMEYVLNASSNAVVNE
                     IILRPQKNRIRRKT"
     gene            complement(21607..22596)
                     /locus_tag="PK733_05580"
     CDS             complement(21607..22596)
                     /locus_tag="PK733_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10048.1"
                     /translation="MIKIAFAGFRHAHINTLYKLAEANPDIEIVAAWEADAGARAAAE
                     KNLGVRFTHDNYDDILKDKNIDVVAIGDYFGIRGSLVIAALKAGKHVYVDKPLCTSLE
                     ELDEIEKLVREKNLKVGLMLDMRYGKVVQPVRNFIKEGRLGEVHAIFFSGQHPLLYGS
                     RPGWYFEEGKHGGTINDIAIHGIDFVEYITGLSFKKVIGARCWNAFATEAPAFKDCGQ
                     FMVELTNGAGLVADVSYAAPNSSGFSLPFYWRFTIWGTKGVMEFTAGSKEIVVALDGN
                     PGTEIIKAPDVDTGDCLSVFVDELAGKPTDIDTSSVFKVTRNTLKIQEYADKF"
     gene            complement(22690..23187)
                     /gene="ybaK"
                     /locus_tag="PK733_05585"
     CDS             complement(22690..23187)
                     /gene="ybaK"
                     /locus_tag="PK733_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOJ10049.1"
                     /translation="MKNEKTNAMRILDKAEISYKTYTYDSSDGLIDGISAAKKMGQPV
                     EKVYKTLVTQGTSKEYYVFIIPVEHELDLKAAARTVDEKAVEMIKVVDINKITGYIRG
                     GCSPIGMKKQYKTVLDSSCKELDTIIVSAGKIGHQIEIAPDDLIKLINCRTASITVEK
                     YSPLI"
BASE COUNT         7467 a         4594 c         3837 g         7633 t
ORIGIN      
        1 agcgtgtgag cctgcctgaa tactgtcttg taatactcat aataacttac atccgtatta
       61 caagacagta atacaatctt tttattatac tggataagaa catttctgat tttttcaatt
      121 tcttcaccat ttaattcagt tatttttttc ccatctatac aatcattaat ctctatatta
      181 tttatgtgaa atttattaaa tacttttatt tttccttcca gatttgtgca ggtattatca
      241 tcaagcagca tactaaaatt atacattttt attctcctca tcatccaaat aatacttcag
      301 tcaacaacac aaatttcgca agtattgtcc acacagccat ttcctctata cctggtataa
      361 tcaatatcag tatcactata caaaagcctt gaccagtcgc aggaattaca attgggattg
      421 ctgcaaatga aaaacagcgg atcatttttt agaatttcca gtcccatatt aaacaacttt
      481 tccatatcaa tcactttaac tattgcattg ccaattttac cctcaattaa tgcatctttc
      541 tttcttaatt caggagttat tctaagaaaa tctctatgtc ccggaggaaa cttctttaat
      601 ataaatatct tttccttata gttttcaata taagttggag caacaatatc cagagtcctt
      661 aagtagtgtg catctatctc ttcttccagg caatgcatta tagtattacg atccatatct
      721 acacccagga gcattatttt cccattgagt tcttttattt tgtaataagg agtaccctgc
      781 ccgcagccgg tttcacaatt attatgacca tctgtaatat attttgcatg ctttcctata
      841 gcagctacag aatggacagg atgtaggctt ctgtacgcat tattccgttt tctaaatacc
      901 tctgaaattt tccctacagc agatggactg gtatcagcat caaaatgatc aaaccatcct
      961 gtaagcgttg acattacaat tgtaccttca tctcctacag tttcaaggag tgcgtctata
     1021 acggaatcag cccctccatc aacataccct atgctgctca atgagctatg tactattaat
     1081 acatcgcctt tgacaatccc cattaattca aggctgaatt ttatatcctc cttgttaatt
     1141 atatcattaa taacatttat gttggagcta cttagcatat ttcctatatt cgccatatca
     1201 tatcaccttc ctgcttttat aacgcatcag taaaaaattt ttctaacctt agcctcggta
     1261 tattctttaa tcttctatta ctactgcccg gcatggtatg gaactggtgc ccggaatatt
     1321 caagggcagt gctgtgagtt taacctgttg tctggttatt ttctccaggt ttatacaggg
     1381 tgcaagcatt aatatatctg cagcataaaa atctttgaaa aatccttcag gtttatcaag
     1441 attttcccat cgtggaaaat ctgttcctaa aaggaaaggc tttttagaaa caagccattc
     1501 catggcttca taagtaaaat atggagattt atcaaggtaa aatttctctc tccagtactt
     1561 tccccagcct gttcctacaa gaattgcgct attcacaggt atgtcaatgc cttcataaca
     1621 ggctgccttc atcatatcag cattaatata tttcctttca acatctgtgt tgatggtttt
     1681 tatttccata ccaatatgtg cagattttct ttccgaaata cccggataga tgttaggtac
     1741 attaaaatct ttttcatcaa ggttaataac ataacatgga atattgacaa gtttctcaat
     1801 tggaatatct gcaacaagat agcatttgtc atttccataa aaatgagccg gagtttccag
     1861 atatgtcccc gtttgagaat gcataccttt aaagatttca caaaatactt ccgcctttgc
     1921 ccactcaact tcacctagtg gttccattct aaatttaggg aatggttctt cataattcca
     1981 cattccctca tatatgggtc ctgttatatc aataactctc atattaagct ccattcctgc
     2041 tgcttacctg cacgcacaat atgttttaca taatgttatt ttaatataaa acattttggt
     2101 tgtaaataaa atttgtcata tgttataatg gcattatgta taaaggtacc agtacaataa
     2161 atatttacta tttggagaat tatcttttta tgaatgacat aaaacgtttc cataaagata
     2221 tgatattgct ttttcttgct gaattgctgt ggcctctggg actggcttta tattcaagtt
     2281 ttttcccaat acatataaaa gagcttgggg gaagtgatat tgttgtaggt ataataatgt
     2341 caattcctcc tcttatgggc gtgctggcaa tagtgggtgg aatttgggct gattttgacg
     2401 atagaaggaa tgtaataata tttggatggg ccattactat acctgcaccc ttgatatggg
     2461 catttgccac tagctggcag tggatgctca taggacagat aatatatgcg ttaacctggg
     2521 tttgctctcc tgccattact ttatatattc ttgattatga tactacaggg aataaaatag
     2581 ccagctatac tatggtatat cttgcagcac ctataggctc catacttgcc cctgcaatag
     2641 gtggaaaaat cgcctcgtcc tatggaaggc aaactcttta tctgatagtc ttcattttat
     2701 attcagtctc caccttatgt acgttgctac tatccaagca gcctgttcaa aataaagaaa
     2761 aaaatgttga tgaatgttac actagatttt caattaaaaa taaactatca gatacccgtg
     2821 attcaataaa acaactggca ggcataatta cttttctgac cattttagtt tcgtctcaaa
     2881 atattggtga atcattcctt tcactttata tgaacgaatt ccgcaatatt tccgttgaga
     2941 atatcgggct gtcttttaca gcgttatttg caggtgcttc actcttcacc tttctttttg
     3001 gaaaaagtga taaaaaaata agttccggta ctgccctcaa tacaggcaac attattttta
     3061 ttatatcagc attattagtt tgctttaccc aaaacagtat ttcacttctg gttttagcat
     3121 tctttttaag aggaataaac cgctcaattg ttgtgtttac acaggttgtt cttgcaaaaa
     3181 atatcaacaa tgaaaataat aaagggttta tactatcgtt atatatagcg gtcagaagca
     3241 tcttgatcgg gctggttaca tacccgggag cagctctgta ccgtataaac cctgcctacc
     3301 ccttctatgc cgaagctttg cttgtaataa tctggatggc tgtttcatac aacagtcatt
     3361 tgaggaatcg tttcatcaac tatgaataat acagctgtct accaccttaa aacaatcatg
     3421 actgcagggt agataaatgt aataaggccc aggattatta acccgaaggc tgctctttta
     3481 ttcttaacct tccagttata tctggcaaat gtaaataaat aaaatgacac aaggcccaga
     3541 attaagataa caaaataatt catgtttact tggcctcctc tgttgttaat ataggtgagc
     3601 ttttcaaaag cgttcctgtt ctgcggatgg caaaatccat atcaatattt acttcagcat
     3661 ctttaaactt cttgatccaa ttaaaatttt gccattcctg cagagtataa aattttgaac
     3721 ttactatcct cccaaaccgg aaaatatcac tatccatttt ctggcacctt tctattaagg
     3781 tattcatctc gttttttatc tgcttaataa atgaatcttg gagtaaggtt ttcaaagggt
     3841 cttcttcata gtttaatctg ctctgtactg ccagaatatc accttcaagc cggattacaa
     3901 catttatata gggttttcca tctattaact ttactttaat atcaggagcc ctctgctctc
     3961 tgacatccag aggaactatt aatggcggtg ccttagggtc ttcaatagta aaaaaccctc
     4021 ttctgaattt tccggttaaa atattcatca gccttgtttc ttctcccgta agttctccaa
     4081 ccatcttgtc tccattaaaa accgcagtac cataaaactc aattttactt ccgccttttc
     4141 taggtatttc gccggcataa tattcttttt ttttcatgtt accagtatct ggtactgtag
     4201 gaccatcttc tctaaagttt tcgaatttat ttattccgcc cagtattgcc gtaggctgcc
     4261 tgtatgtgga ttttaaccca ttatagaaat catttaaagt tatatgagca aaaaaaccta
     4321 tattcgccgg ctcagctact accaattcca tggctttaga aagagtagta ccaactacag
     4381 gctcatattc ggcaagaaaa tcagaagcct ttcctcttgc aacaataaca tggagggtcc
     4441 ttcttatctc tctgaatctt attattggcg caataaattc acctacaccc gtttttgcaa
     4501 tttcttcgga aaaaactaaa aacttagcat gcataaagtt taatttacga ggaacagcag
     4561 cattaatcat attaatacct gtaaaaaagg atagtgcatc tatggttatt atttcatgct
     4621 ctccacctgc cattccacca cctcccccgc ctttcccccc tgcacctcct cccccggctt
     4681 ccttcattgt atgaaattta acggtaagcc tataggtatc tgcaacacct ttatctactc
     4741 ctatagcaag aacataggtt aattcatcaa tttcttttgc atcatagcaa cctgcaagga
     4801 taaatatcaa caataaactt actataaaag cctttaatac ttgtatcaaa cttgtatttg
     4861 catatcttcc aacctttaat gacttcattt taagcaccac tcctgctgcg tgtgaaataa
     4921 attcgtcctc ttacctacga acacttctaa acactttttt tcatcctttt cctaccctct
     4981 cctcttctgc ttctcttttc ctttcctctt aaccaagctg caataagagc aataaaagcc
     5041 ggcacataat acactaacca tgcatattct cttagagtgt ttacatatcc caatgcaata
     5101 ctttccaggt ctttaggcat catagctgta gcaaaagcaa taattgataa gggaataata
     5161 ataggtccaa tgtcctgtat tctaaaaatt ttactgtata tgcttgctga aatatacaac
     5221 tgaattgaaa cagctattaa agtactgata ttccatataa agaggaataa aggatcaagt
     5281 ctttggaaaa accttccaaa gtctataaga gctgtcatct gatatagggg tgatgttacc
     5341 tcctgtgcaa tataatatgg aaaggtgagg gaaaatgcca ataatgcaga tgatattaat
     5401 atacctgaca atattatact tgtgtatcct accattttta cgtattttgt cccttgaagg
     5461 gatttagcaa taattactgc aataattgca tagccatata cagagcttct gaaaagccca
     5521 tgtattacaa catttccaag tccgtatcca aaaactggtt ttattctgtg aacttgataa
     5581 tttggagccg caagtataag ccctgcaata aaacttatta aaagaacagg ggcaatgagc
     5641 cttgcaaatc ttgcctgggt ctcaagccct aaaataccca aaactatcac acctaaaaca
     5701 aaaattccta taatataact gggagggctc aaagggagaa cataaacctt cataacctca
     5761 gtaaattctc tcaaaactat tcccgcatca attataaata ttaaaaaaac agcaaatgag
     5821 aaaaggctgc caatgtaagg tcctaatgtt atttcataaa tctctataag atctttatca
     5881 ggaaactttt ttatcagcaa atatataatg gtgaaccctg ctgcagctgt tgcagcagat
     5941 attaaagtca tataccatcc agcagagccc acaaaccttg ttgtaatgct tggacttgta
     6001 aaaaatactc ttataactat tgtaattgtt attaggctta tggcttcctg gggaccgaat
     6061 ttgccctctt taaccatttc tctctgtctc tcctttaaga tcatttattc ttatttgttc
     6121 ttattatctc cccattttct ggggtttgca gatgcctttc tccttttaag ggtgtttaaa
     6181 gcatccggcc tttctgcagg ctcatacatt ggctttttta gtattagttc tgcatcactc
     6241 attgtctttg gagctaccgg actgacaaaa ggcacaccaa aagactttat actacataaa
     6301 aaccctccta taataacaat acctactgat attccataaa aacctgctat agctccaaaa
     6361 aaaataagga aaaatcttaa tattctagtg ctaattccca gtgaaaagtt cggaagagca
     6421 aagcttccta gtcccgttac tgctacaaca ataataagaa taggactgac tatattggca
     6481 gcaacagctg cttgtcccag tatcaatgcc ccaataatac caagggttgt accaataatc
     6541 ccaggtaccc ttaatcccgc ttcacgaatt aactcaaaag atagttccat taaaatagtt
     6601 tcaacaacta caggaaaggg aacattctcc ctggacttgg ctatagctgt aagtaaatca
     6661 gtgggtatca tttcctgatg atataataca agggcgatat atgctcccgg caggaggacc
     6721 gatactaaaa gcccgaaaac tctaataatt cttattactg tcccatactg ccacttcata
     6781 ttagattctt cagaagtcct gagcatattg taaaagttta tgggtacagc tagagcaaat
     6841 ggggtaccgt caacaataat aaccactttt ccatctatta aaaatgatgc cgctctgtcc
     6901 ggcctttctg ttgataatgc ttgagggaaa atagaagaag aatcgtcctc aatgagctgt
     6961 tcaatcattc catcaccatg tacataatct atatccaggc ttttgattct tcttttaact
     7021 tcattaacaa ttctgggatt ggtgatcccg tcaatatata atattccaca gtgtgaatta
     7081 ttttccctcc ctacaggcaa cacttcagta acaaggtgac tgtttttgat aatacgcctt
     7141 attaaagtaa tatttgttct aaggttttcg ttaaaacctt cctgagagcc cctgataaca
     7201 ttttctgtac tcggtctttc tacccctctc ttttcatacc ccctggtttc aaataaaata
     7261 cactcattgc agccatctac aaacagagcc gtaaggccat ttaatatctg tctgataatc
     7321 atttcataat ttttttctct ttttgtgtca tgtactgcca gcacattatc ttcaatatat
     7381 tctattgagc attcttcatt tgataaaccc tggaacagtt ccggcagcat aagctgacgc
     7441 agaataaagt tgtttatagt ctccctgtct gccataccat ctataaaagt aataaatgca
     7501 tttatttgtt taccgatttt aaattgtctt ataataatat ccgagttttt attagaaata
     7561 aactcatctt gtattttttt taaattagtg tacagctttg ggctaacctt tttaatattg
     7621 gctgtacttt gactgtcttc ctcacccctt ccctcaccct ttccctcatc ctttcctgaa
     7681 ctcccagact cttcaaaagt aggttctttt tccccaacct tgtcttcttt gtttttggct
     7741 tgattccatt catccacatt taaaggtttt ttactttttt ttacatcatc tgtttcacgc
     7801 ttgtcttgag ttttctcgct atttttactt tcctgattac tttgctgtac attttcctgc
     7861 tggttttttt gggtaccttg cctgatattc ttctttttat cctcattctc actgtttttt
     7921 gattcctgct ccgctgtctt ccttttctgg tcatttattt tttcatcata accatcccgc
     7981 tccgtccctt catattcagt ttcaaggagc tcaaagccct caacatcctt gggaccattg
     8041 tatgttataa ggtttcttag aaatttaata gtgttactag tgttgctcaa ttatatctac
     8101 cctttcatct ctaagcttta gtaatgctgt attcatattg cccggaacaa gttaagtttt
     8161 ctgctgaagt tgctatagaa tattatttgt ttttttttca tatatattcc tattaaactt
     8221 tcttcttatt taacaatata gaagacttat aagagaagta ataccttata ccaggttttt
     8281 attgaggcta ctttccagat aactgtctga ataattttga tacttgcaca agttgaattc
     8341 ccattttttc taattgaggt attgtttctt ttattgctct ggcagtagtt ctacctcctg
     8401 cagagcctac atgtccaata actacagcat agcctttttt aacagcaagc tcacctgcag
     8461 tcaaaagctg ttgcctggca taagccatag tttttatgga ggattgctca agaaaataat
     8521 ttctctctac aaaattaatt tttatatctt tagctacttc tctgcaaact gatttgctgc
     8581 ttgtcatact atctacaaaa tacaggtttt tttcctccat attcttcata acgcaggaca
     8641 taactctggg atcttcactg gatttggtac ccatatgaat attagctcct acagcatgag
     8701 gtacaactct gaaagaatct aatactatcc tgcgaatttc attattactt tgaccaagct
     8761 taataggtct aggcccaagc cagcttgcaa tatctctatc ttgagattgc ataggaagat
     8821 gcactattac ttcaaatcct ttctcatggg ctttattggc atccgctttt gtatatgtga
     8881 gaaacggcat tattgcaaat gtcataggtc tcccaatttc catcatttcc tttaccccat
     8941 ccctgttttg gccaaaatca tctataataa tggcaagttt aggttttgtg taattactgc
     9001 ttacagaata atttataaaa tcatcctcat tattttctat atctcccgta taaatattaa
     9061 aattttgttt tgatactatt gcgaaaacta caactataag caatataatg actatagata
     9121 ttacatataa ggttttttta taaatatcaa gccagtcctt atttttcttc atgagtatcc
     9181 tccaaagtaa taaattattt tgcataactt ttttagtaaa tactttaatt ctacaatgcc
     9241 aatttgggaa acgatgtctt cctggatatt tttttaaata gcataatgat agtattaatg
     9301 tttttacatt taatatgcta tagataacgc acaaaagaaa ctacactata aaagttttgt
     9361 acgcttatgt taccttaatt aatacgtaaa aatccaattt gttccttgta aaattatttt
     9421 tgcgttatat ataattagaa tataaaactg aaagtaaaga attgccgctt tcttcataat
     9481 ttagattatt ggtgcattcc tcaaattcat tgaatcctgt ttttctttcc agttactttc
     9541 tttgttattt tacaatatag aaggcttaca agtagaataa cacctccata gcccataacc
     9601 gggtaaagat aactgaccag atttgaaaac ccgaatctgc ttgtgataaa agcaagaaat
     9661 gctgtaaata ctatgcatgt ttttccagtt aagcttgcta acctgctaga accgattgaa
     9721 ttcattaagc tgtcggaatt gataattcgt gctgtaaagc cgtagaggct tcctactgcc
     9781 gttgtgtata tctcagctaa aagcacaata atataaccta cttgaactac atgagatatt
     9841 tcccctgcaa taaaagccat gggtacttcc atatggatta tacttcccaa atttcttacc
     9901 agagtaaagt atattgcagc accagttgct ccaaggctta ctcctcccag caacgcacct
     9961 tttttgatag attcattgtt ttggatatta ttccccagag gagcaagaac agaaactgat
    10021 aaaattatat tatatgatgc atataatata gcagcccaaa gccaactgcc tattaaacca
    10081 ttacctgata ccttatgtat atgagtataa tccggatgaa caaaaaatat ggaagcaatg
    10141 ccaacaccaa gagcagcaac aattaagaaa ggtacaataa aacttattga gtttataacc
    10201 ccttctatac ctgtaagtac tgtaattgca gttataacag ccatcacaag gttgccccaa
    10261 acagcaggta tattaaactg ctgtctaaaa attgcaccag accctgcaat catagctgta
    10321 agggatccaa aaaggaaaaa aattatcagg aagtcaataa caggtcctaa atatttaccg
    10381 cctgcatatc ttattatctt aaggtgggaa gtagaaccaa ggtacatacc aagttccata
    10441 ataatatggc catatataac aaataataca gtgaccagaa ctaatcccca gaatcccttt
    10501 atcccaaata ccgagaaaaa ctgaagtatc tcctgtcccg aagcaaatcc tgcccctacc
    10561 acagttccga tatacgttgc agctacttta aatgttaata tactttttgc atcgctgcac
    10621 cttctttctt ttgcgtatat gttattattt ggtgctcggc tgtgatttct tatagaaccg
    10681 ccttttccgc ctcttctatt ttcatatttg tacatgctca cattaaccgc cattctgcca
    10741 ccatatatcg gcattaaatt atatcaaaat ttgttttttt aactttttga ggaaaatagt
    10801 atacagcaag tttgtacatt atatacttat gggatagttg aaaggaatat gtgacaatat
    10861 gtgaattttt ataccgatta cttatttctg ttcattttat gataaaataa aattaaagac
    10921 tatagataac gcacaaagga aaccacatta caaaagtttt gtggcccgcg acagattatt
    10981 tttaaagtta gcagaccaca gcatcaagag agaagatttg taacacttga ataagaaata
    11041 taaaaataag atgttacaaa ctgctatata atttacgatt tgaaagaact aatgaagaat
    11101 ttgcaaatcg taagttacat caaggtaaca gaagcaccta aatggaggta cgcaaaaatt
    11161 caatttattc catgtagaat catttttgcg ttatatataa tatgaaaagg tggaactatg
    11221 gataaagata aaattttaat agttgatgat gaagatggaa tacgggaaat gcttagagaa
    11281 tatctatctc ctgaaggata tataattgat gaagcctcag atgggataga agcactggaa
    11341 cttttcagga aaaataaata ttctcttgta attctggata taatgttgcc taaaatggat
    11401 ggatggatag tgttgagaga aatccgcaaa atgtcacagg tgcctgtaat aatgctctca
    11461 gcaagaggtg aagaatacga caagcttttt ggttttgaac ttggcgtaga tgactatata
    11521 gttaaacctt tcagtccaaa agagctgctt gcaagaatga aagtcataat acgcagaagt
    11581 accaatcctg cattgtcgga aagtaaagaa aacagtgtaa aatatgaagg attagagatc
    11641 gattttgatt ccagaaatgt aactgttgat ggaacttctg taaatcttac tccaaaagaa
    11701 tatgaacttt taaatttctt taccatgaat ccaaacaagg tgttctccag ggagcagctt
    11761 ttaaatgagg tatggggata tgacttctat ggagataaca gaacagtaga tactcatgta
    11821 aaaatgcttc gtgaaagcct tggggggtat agaaaattca ttataactgt atggggtaca
    11881 ggatacaaat ttgaaacagg agtgagaaaa tgagaagtat tgcggttaaa ttatggacag
    11941 ctatgatgct tctgataatt ataatgttag ctctgctctg gttttttcag atagttttcc
    12001 ttgaaaactt ttatattaat cagagagttg aggaagtaaa aaatagaagc attttaatta
    12061 tacaagatat aaatgtcacg ggaaaagaag aactggaaac caggcttgaa agtcttatat
    12121 atgattataa ttgtaatata gatcttatag acaccgcagg taaaatcatt tacagtaatg
    12181 ctgccggaag gcaaatgccc atgatgggtg ccggtcatat taaaaaagaa ttaatgagtg
    12241 aagttttatc aggtaaaact gtaactacat ccctgattca tcctagattt aatagtaaat
    12301 acatgcttat aggtattcct gtaaaatctt ccactggtga agtcaaagaa attacagaaa
    12361 tcacaggagc ccttcttata aacatgcctc ttgcaccagt agcagatact gcaggaatac
    12421 ttaaaaaaca gcttgtttac atttgtttcg ttctgttttc cgccgcactc ctgctatcat
    12481 ttttattatc aagatcattt ataaaaccaa tacttaaaat cacggaagct gctgataaaa
    12541 tggcctctgg aaatttcaat gtgagaatta gttcagaatc aaaagatgaa attggtaagt
    12601 tatccagggc aattaataat ctgggtgagg aactttcaaa aatagaacag ttaagaagag
    12661 atttaattgc taatgtgtct cacgagctca ggacacctct aagcttaata agaggatatg
    12721 ctgaaacaat aagggatgta acaggaaata acaaggaaaa acgggaaaaa caaattggaa
    12781 taataattga tgaatcagag aggctaaaca ggattgtaga tgatatactt aacctttccc
    12841 aaatacagtc agggaatgta gtattaaaca ccacaaattt taacttgaac agaacgatca
    12901 ggaatgttat tggtaaatat gagattctaa gtaatcaatc tggaatttcg gtagtttatg
    12961 caactaatat aggtgacaga aataatgaag tccccgctgc caatgcaaat atcatggtaa
    13021 agggagacga agcacggata gagcaggtac tttataactt aataaacaat gcatttagtt
    13081 acagcaagtc aggtgattca ataacagtta aaactctagt tggcaatggg atagtaaaag
    13141 ttgaaatatc agatatgggt gagggtattt cgcctgaaaa cattaagcat atatgggaca
    13201 ggtattataa gtcagataaa tcggttacaa gaaaacagat aggtacggga ctgggactgg
    13261 ctattgtaaa aagcattctt gaggttcaca aaagcagata tggagttgaa agtgccttgg
    13321 ggaatggtac aacattctgg ttcgaactgg atctttcata ataatctgaa cgattcaagg
    13381 caacataaaa gtttcatttt tctatcatac ttttatcata attacctttt ataatgaaat
    13441 catcaaataa atcaagggag agtgatttca atgaagaata tgaaaaaatt tgcagccgta
    13501 ctggcagttg tagcaataat tgcaacagca ggagcagtgt tcgctgcgga ccttaaaaca
    13561 cccgtagaaa ttgtttccgg attaactggt aaatccgtag aaagtcttag tcaagaaaga
    13621 gcagcaggaa aaacttatgg tgctatagct atggaaagtg gaaaacttga tgaatttaaa
    13681 gtacaaattt tagaacagaa aaaagctctt ctcgaccaaa aggtaaaagg cggaacacta
    13741 actcaggaaa aagccgatga aatctataat gcaattaaag aaaatatagc aaattgtgat
    13801 ggttccggag ctacctgcat tgggaggaag tacggtgtag gcttcggtag tggcagtgga
    13861 gcatgtgcag gtaatggcat atgcaatggc accggagcag gtaacagcac atccaccggt
    13921 atcggagcaa gcaatggctt agccggcggt atgagaagag gaaaaagtgc gggcatgggt
    13981 atgaagcgca gccttcaggg tcaaggtcag ggtagaggaa tggggcttgg cagataacct
    14041 accattatta caaaccccag tataagaatt ttttaggcta tctataaatt cctatactct
    14101 caaaaagttt tacaagcttt tatgtaaata gccatgtaac gcaagcatta catggctatt
    14161 tgcaataaaa ttttaaacag aatatcttat tcaatataaa ttacctcaga atcataatcg
    14221 taattaaata gttccgagta tcttccccat gaagtaattg tttccataag aatatcaacc
    14281 tcctggggag taaaatgatg caggaaaaga tcaacaaaaa actcttcttt cattgaaaga
    14341 tcctttttat tatttaaaac atttaatacc accttaaata caggtaattg tttaattctt
    14401 tccctgaaaa tcttttttct tccgtccata tcagaatcta caaactcctg tccaagaggt
    14461 aaaataatgg catctccctc tctaacctcg gcaaaaccca acagtgcaat agcttctata
    14521 cttggcagca attcatcact ttctattaat aaatctctgc caagtttgta aatatctatt
    14581 ttaccatcat tatcttcaag tatttccaaa agtcctgcca gtactcctgg atgagcatgc
    14641 ggcaagtttt taagtttctc ctctccagta tcggatatct gatttttagt cttattattt
    14701 gtgctttcta tttcttttct tgtcataact ttataaatat ggtctaccat catttcaaat
    14761 tcctttgatt tcctgtttct tgggtgaggt aaccgaattg gaatttccgc cagtacagtg
    14821 gcagggcttt tacttagaat tatagctcta tccgacatca aaacagcttc ttcaatatta
    14881 tgtgtaatat ggataacacc ctttatcggc atccttttct cctgccaaag ttcaacaagc
    14941 tcagttctta aattttcagc cgtcaaatag tccagggctg aaaacggttc atccagacat
    15001 aatagtttgg gttctactgc caatgccctt gcgaacccga ctctttgtct cattccgccg
    15061 gaaagttcct tgggatatgc atcttcgaaa ccgtcaaggc ctataaggtc cagtagtttc
    15121 tcagctttct cctctgtata atccttggga aatccttttg cttttaagcc cagctctaca
    15181 ttttctatta ctgtaagcca tgggtataag gctgcacttt ggaaaactat agcagcaccc
    15241 tgattggaac ctttaacctc ctttccttca aatattactc taccacttgt aggttccata
    15301 agtcccgtta taaccctcag caaagttgat ttgccagagc ctgaaggacc aagaatagag
    15361 acatattcgt tgttgtttat tgtgatattg acatctttaa aaacaggtat ttcactgtca
    15421 gcaaattttt tagtcaaatt ttctattttt atgaattcct taaccgctgt catccaaatc
    15481 cctcctttta tatactgggt aatagtcaag ttatatgtca agtctgaatc tttcttcaga
    15541 aaacctgaat aactttttcc atataaacct gttcgttcct acaacaataa ttatcataac
    15601 caatgtgctt gccaaaagca tgggataatt gcctatatct gtagattgtg ctataagagc
    15661 ccccaatccc agtgttttca tatgctctcc gccaaaacct atatattccg atactatggt
    15721 agcattccaa cttccacctg ttgctgtgac taaccctgtt aatatgcttg gaaaaatagc
    15781 tggcaagatc agggttttcc actttttaaa ccctttaagc cctaaattag ctgctgcttc
    15841 ctttagatcc tcgggtatag acatggcccc ggcaattaca ttgaaaagta tataccattg
    15901 tgtacctaaa agcatgagtg ccaccgatgc aatattcaga cctcctgcaa actttaacag
    15961 tgctatcagc agcaccggaa aaagtgcagt agctggtatt gaggcaacaa tctgtattat
    16021 aggctgcagg atattagcca gctttttatt aagtcccaca gcaacaccta tgggtacagt
    16081 ccataaaaga gctattattt gtgctacaat aacccttacc agcgaagcca atgcagaggg
    16141 aaaaagctgg gtaagctcac ctccggacag ttttagaagc atttcagctg ctctaaacgc
    16201 cccatatgca aatagcaata tggcagctat gcctaataca ttataaaccc actttttcaa
    16261 agcatccctt cttgccttgg aacattgatt cataccgatt tcatattcag gtgtgcccct
    16321 cggactaaat aatttgttaa gaaattcatc tatatacctt atcattggac gagttacctt
    16381 gttagataaa gtttcgatca atactgatct tcgaataata taaagtacaa cagattccgg
    16441 tgcatctcct ccgcctgtaa gttccacctt gaacttatcc gaccaggcaa taataggtct
    16501 ccaaaggagc tgatctataa gaacaataag tattatcata gtaatcaagc catgcaaaag
    16561 tgcttttgtg tcaccttgaa aagcagctgt ctgaagatat gagcccagcc ccggcagcct
    16621 gaaatcattg tctttcaatg taaacatttc gcaggccatc aggaaaaacc atccaccggc
    16681 ccatgacatc atactgttcc ataccagtcc aatagctgaa aatggtagtt ggagtttctt
    16741 aaatttctgc cacttgttaa gtttaaatgt actggcaact tcatcaagat ccctgggaat
    16801 agttgtcaat gaatgataaa agctgaatgt catattccac acttgtcctg taaatataag
    16861 aataatcgaa gataattcca caccaaggct gctgtgagga aatattgcaa caagtcctaa
    16921 tacaacagcc ggaagaaaag ataggacagg aatggactgc agtatatcaa gcaaaggcag
    16981 cattatcttt tcagcttttt tattttgtga agctacatat ccatagatta atgtaaacac
    17041 taaagacaaa atataagctg ccagcatacg caaaaatgaa aacagcgcgt aggcaggtaa
    17101 atactttgtg ttaagtgata taacaatgtt ggatttatat attgggttat ctatatttaa
    17161 agatataagc aaatacatta cagcaataat tactgcaatg acaactccgt cagcaggagt
    17221 tatgctgtga aacatcaagt ttttttcttt cttcaatgtc caacccctcc ttaaaaaatt
    17281 tctatataga gggatatgtt aaccaggaga atgtcaacaa atccccagat atagtattga
    17341 tgtaatttat taattccatg taacataaat cagggtcaca attatcactt tccattctcc
    17401 cacctccttc ataataaaac aacataaaaa aacctctcaa caaaagaaag gttttctgta
    17461 atcaaccaat accttcgttg agttttggca ctatacagct ttgagataaa cccagctgca
    17521 ttaagtaaaa ccttaattcg gcaatacctg ttgacccatt ggcatctctc gatgtttccg
    17581 ggcagcagca tttatctata taggagcctc acctaacata cactatttaa tttttgtcta
    17641 tcacacatct atcatgcatc ttatttaaat tatacttcaa ttacttagtg acttcaagcg
    17701 aatttctata gatttagatt tatagcagcc tttgtgcaca atgtatggaa tctgtgtaat
    17761 ctatataatc taaataatct atatacatag ggcctgctga tacattcata tacctgctaa
    17821 aatatccttc taatatatcc taataatagc caatattatt aacagtatcc cgggaacaat
    17881 agttataatt ttttgattaa atttctccat agccgttatt ctttttccaa gaaaggttcc
    17941 aacaaataat aatactaact gaaataaacc aactgcaagg ggtaaaaacg ccgaataata
    18001 tccagacagg gcacttccta taccaacacc aatggcatcc aacgacaatg caagcccaag
    18061 caaaatagat tcttttacat caatagtgcc tgatttatct atatcacact ctgacggatt
    18121 acgaataacc tgtatagtga ttcccaatga ttttatggcg acttttatta atgtttcatc
    18181 cttaatatct aaaatattta aattattttt aatatactta ttggccttga tatctacatc
    18241 agatttggaa atatccatgc ttttattttc aattaaacca tgaataataa tccagcatcc
    18301 cataagtaat aaaatgaaaa tacctattaa ctttgatata cctggaggta tgatggaaga
    18361 tatatattta ccaaaataga cagatgcgcc tgaatataat atggaaaaca tgcaaataaa
    18421 aacagctgac agtaccggta ttttaatttt tctcaatcca taagctattc caacccctaa
    18481 agcatcaaga cttaaagata atgcaagaat acatattgta gataccatat ttattattct
    18541 ccttatattg tctgtatttc aggttcttta cccatacaat atatgacaat ttataagagg
    18601 atgttacagc tggatattgt aatgcttcct tttacccttc ttaccgggta gtagtgtaat
    18661 attgaaacac tgcttataaa tgagtataat ataggtatca ggggacaaca acctgatttc
    18721 agcatattgc aatataagga ttggaggttt tattcgtttc atggtcttta ttgtaacagc
    18781 actcatgata gaagctgctc ctatcattaa gcattttaaa ttgaaaaaag atatgtctgt
    18841 gcataaatat caagtatata agaattcggc aattgtactg attataagcg gtgtaggaaa
    18901 agtaagaagt gccatggcat cagtatattt actttcaaat cacgaaataa ccggtacgga
    18961 tattttcctt aatattggtt tttgtggtgc cggcagtacc aaataccctc caggaagcat
    19021 gttacttata aataaaatta ctgatataga cacaggaaaa gattactatc ctgatgtttt
    19081 tataaatcag gctctaccaa gggaagccat atgttgttat tccaggccgc ttcacaaaga
    19141 agacctgaaa agtcaaacag agattttttg tgatatggaa tcatcaggaa ttatggaggc
    19201 tgcaaacaag tttatatatg ctcatcaagt ggttatatta aaagtaattt ccgactacct
    19261 gactcccgaa aaccttaata aggatcaatt acaaacctta cttgaaaatc atatgcccta
    19321 tattgagcaa ataataaatg agttacagca gttaaatgag ctattacagc tgaatgaagg
    19381 tttgaatgat agatgtagca agtttacttc aaaagaagaa aaggaagtta ttgctattat
    19441 ttcagatatc tcagataatt taagattttc cgtgaccatg aagcaaatac tctttaaaga
    19501 agcgaaaaaa gcaaagcaga aagggattga tccgttaaaa atccttaaac cattaacaga
    19561 aacaaaagtt aactctaaag tagaagggaa gaagattttt gggcagctca ttgaaaagct
    19621 tgaacaaacg cttgtttaac acaatttacg tggaagaagc agcttatgga tatactatta
    19681 caagggaaat gctgcagaaa tatcctgatt gtccggtaat tccaatccgt cactataagg
    19741 atgttttcaa ccgtacaaat cagcattatg ctatccaaaa gcagtatccc tcattgatcc
    19801 tggcagtgaa aggcaatcag tttttgtaca agggtccggc ggtttgtcaa gattttgggc
    19861 attccggaca ttctgccttt tattatactt ctttcttgtt gaattgcatt tttaactgcg
    19921 aatactgtta ccttcaggga atgtacccat ctgccaacat tgtagccttt gttaatgtgg
    19981 aagatttcaa gtttgcaatg gaacaagcgg ttatgaaaga acctgttttt ctagcaatat
    20041 catatgatac tgatttgatt gggtttcata atataatccc atattacgat tatttttgtg
    20101 acttttttgc atcacactcc gagctttatg ttgaaatccg tactaaaagt gcaaatcaaa
    20161 ccttttatca gaatcatact ccactggaaa atataataat tgcctttacc ctttcacctg
    20221 aagaggttat acaaaaatat gaaagacata ctcctgcatt ggagatgcgc attaaagctg
    20281 ttcaggtagc tataaagcat ggctttaagg ttagaatttg ctttgaccct atattcattc
    20341 atcctgaatt ggataatctc tatgaaccct tctttcatta tatttttaca caaattgatc
    20401 ccgggagtat acaagatata gggtatgggt ttttccgcat gtccaagcat ttttttaaaa
    20461 gaatcgaaaa acaccagatt aattcacagc ttttttcgga aaaatattgg gttgacaagg
    20521 atgtggtatc ttattcaccg gaacttcagg agaagattaa atcaaagcat tttggcattt
    20581 tgacccaata tgttaaagaa acttctatcg taaccattta gtataatatt ttgagcaata
    20641 cttaatcttg ccttgcaaat aattattaat tttattttgc aaataattga taaaggagat
    20701 ttttctaatg aaagtagcaa ttttaacagg agcttcaaaa gggatcggtc ttgctttgac
    20761 gaaaagactt atttccttaa actataaagt ttatggcata tcccgttcat ttcaaaatac
    20821 accattttcc catgcaaatt ttattcgcgt tgaatgtgat atacaggaca caggtaaact
    20881 tatccaagtg atagaatcta tcgaagaccg ggaagcaaat ataaatcttc tggttaacaa
    20941 tgcaggaata ggatacttcg gcccccatga gcaaataaag ataaaggaaa tacagactat
    21001 gacacgtaca aacctggaag ctcctctgat tttatgtcag ctccttttac gggcactaaa
    21061 aaaaagtaat ggaacgatta tttccatttc ttctgtaaca gcaaaaaaag taagtaccca
    21121 tggttgtgcc tatgccgctt ccaaagccgg acttacccac ttttttacca gtttgttcga
    21181 ggaagtaaga aagtcgggtg tcaaggtcac aaccattcat cctgatatta caaaaagcag
    21241 tttttatgat tcactggatt ttacatatga agacgaatca gatacagttt tactggatga
    21301 gcaaatagca gatgcaatgg aatatgtact aaatgcatca agcaatgctg ttgttaatga
    21361 aataatcctg cgtccacaga aaaaccgtat tagacgtaaa acttaagcat gtgttactaa
    21421 gagtctactc ggaatgtcta agctataaaa gcgaaaagca gactacagat tgcagactgc
    21481 agactgcaga ctgcaggaga aacgctcata gactgcatta tgatacaaga ggatgcaaga
    21541 aatgacgttt ctcctgtatg agcccgcttt ttcggacaga ttcctaagca ctaaagccta
    21601 cctgcactaa aacttgtccg catattcctg aatcttcagg gtatttctcg taaccttaaa
    21661 gacagatgat gtatctatat cggttggctt tcctgccagc tcatctacaa atacactgag
    21721 gcagtcacct gtatcaacat ccggagcttt gatgatttca gttccaggat taccatcaag
    21781 tgcaacaaca atctccttag atcctgcagt aaattccata acacccttgg tcccccagat
    21841 agtaaaccgc cagtagaatg gcaggctaaa tcctgagctg ttgggtgcgg catatgacac
    21901 atcagccact agccctgccc catttgtcag ttcaaccatg aactggccac aatccttgaa
    21961 tgccggagcc tcggttgcaa aggcgttcca gcaacgtgca ccaattactt ttttaaagga
    22021 aagtcctgta atgtactcta caaagtcaat accatgaatg gctatgtcgt tgattgtgcc
    22081 gccatgcttg ccttcctcga agtaccaacc tggtcttgaa ccatataaca gtggatgctg
    22141 tccgctgaaa aatatggcat gtacttcccc aagcctgcct tcctttataa aattgcgtac
    22201 aggctgtaca acttttccat atctcatgtc cagcattaat cccactttga gatttttttc
    22261 tctgaccagt ttctcaattt cgtcaagttc ttcaagagat gtgcataacg gtttatcaac
    22321 gtagacatgc ttgcccgcct ttaaagcggc aattactaaa gaaccgcgta ttccgaagta
    22381 atcaccgatt gctacaacat caatattttt atcttttaat atatcatcgt agttatcatg
    22441 ggtgaaacgc acaccaaggt ttttttcagc agctgctcgt gctccggcat ctgcttccca
    22501 ggctgccact atttctatat cagggttagc ctcagcaagc ttatacaatg tgttgatatg
    22561 ggcatgcctg aaccccgcaa aagcaatttt aatcataata ctcatccttt cttctcataa
    22621 gtatattatt atggctcatc cggacagtaa ttccgggaat atccggacac attgccgtat
    22681 aagttaattt tatattaatg ggctgtattt ttcaacagta atacttgctg tcctgcaatt
    22741 aataagttta attaaatcat ccggtgctat ttcaatttga tgacctattt tacctgcact
    22801 tacaataatg gtatcgagtt ctttgcatga actatccagc actgttttat actgtttttt
    22861 cattcctatt ggggagcatc caccacgaat atatccggta atcttgttaa tgtccacaac
    22921 ctttatcatc tctacggctt tttcatctac tgttcttgcg gcagctttta aatccagttc
    22981 atgttcaacg ggaataataa aaacatagta ttctttactt gttccttgtg taaccagagt
    23041 tttgtatact ttttcaacag gctgtcccat tttttttgca gctgaaattc catcaataag
    23101 tccatcacta ctatcataag tatatgtttt atatgaaatt tctgctttat ccagaatccg
    23161 cattgcattt gttttttcgt ttttcaaggc aaatcctcca atatatattg aattataatt
    23221 tattatacta attgcttaat aatttttatt attacattta gccgtataaa ttacaatatt
    23281 aaaggctgta ttgggtgcat attttatttt tgaagaaatt attgaaagaa gcattagtgc
    23341 aggtactgta gctgggcagg ggaaactctt ctctcaagta acagatattc cttttaggtt
    23401 gaagagtttc cccgtatcgg agcgaaggaa gagcaaaatc agtgtttgaa caaaataatt
    23461 acactttttt attcagatta ttttgtgagt ttgattttgc ccgtagctga atttaaaggc
    23521 tctaagtacc g
//
LOCUS       DAOSDD010000041        23244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_972_length_23244_cov_5.332183, whole genome shotgun sequence.
ACCESSION   DAOSDD010000041 DAOSDD010000000
VERSION     DAOSDD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23244
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_23244_cov_5.332183"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4259
                     /locus_tag="PK733_05590"
     CDS             <1..4259
                     /locus_tag="PK733_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOJ10050.1"
                     /translation="DYGENNTQEYLNGIVFVPVRVTGTLMDTIFYNNPEEREKRYNRD
                     DIHSWTMSLNETTYPNIAPDDSGPSGSYKNKKDAIITLWFKRSEIFQRQTWKLNAYSE
                     ALFLDGKSSKRNATGTLLFTIKAAPQNQEPPQEPPPEAIEEPPPPPVIIEPDCHIPHP
                     GFDIVPYPAYDNTDLSQVLSRKVYINGDEVDDVEFFSGNYIFGDGNDGIKKIDVIYES
                     MDEITSSTTQWAYIYDTKPTAQYKLEGAFKENRKLLASENCASGNTQIVLDKYPIVSY
                     NWKFISIDGESSSLKQRTPTSDKYREFLYKKPGVYRLELTVTNTLGRVSKPYTLDFVI
                     YEDTPPAIEMNIWNGVLTRGEELKLHYSASSVDNDSIALKTLQLYYDSNNDGECDQLV
                     KTFPDGEFAGYAPDKLGKYRLVATATEEFGQETLPEYTTVADKKKKTAESEFWVDNLV
                     PMTDIYVNVPVVKPEIDLYVMMDKNLNESIKQYITANRINFNNFLRTKNILPKVETWD
                     MKTYEYSQSVSDTYRSGESYPPSSILYSSGGYSGTLYLEPDSTVDNGEDRDFGSWDTK
                     TESKEFTRSHSNTITEEWWDGSLYRTTESDPEPSSYDVDEDGYSGSIPQVSRDGPYNR
                     KVDNYYSDREFIIHHSNTVTDCYNDGKLVYSSNSDPAPSSYYINEDGYSGYIYRDDTE
                     GPYNEKHWFEGQYEYYEKTWVAVFRGTLSKIHAWDIHETYISTYRGTLTKSKQVWVPD
                     MRWVSDYIGYYSGIVYKYVRQPYSNPFRATSDKYVVYISDGNINELTDLGMVMSKADA
                     RLILVGANSIRNQMAHDHYISRDKPMEQIMQEVLDYIAINSPAIEKHYVLAGVDIFDI
                     KAIDFDDENDPIIERKFQYVQDANYFDNPTGMENFAAASFSENSNWVDTKVGKFNEVG
                     EFRIFRRIKDQPSPDPNFANYSYFSGTPELRVYAHRKPIAKASLEWDYDNSKGVYKTR
                     WVCQSYDFDHQYSREDKGIAERKIMFRRTGGEWYYYVPEELSSGTYELQYYVKDIEGA
                     WSDPFAMNFTLLSAPSIQFDAKLCTKDSKFSLNNIPASEDLEVYDAWTRYPYNVKLEM
                     ALYNGSIRISPVKSVNYSSSTGVKTGNDIQWNSISYQVPNTIKDGTYTFKVEAVDTNN
                     SSIQAVKSFSVMVNTPINLVPSMPETVMTGNTADIKAATEKYASVTRVAMFYGTSYEQ
                     NLELSSVLQEDMKEWSNKYTIPSNIPEGVYTARFISTTQNGKSEMKDATFKVETLKIS
                     KVTIEGYWNHWRGQVDMFGRQLANEPHRFLSLERVKINVQTDGFADKVEIRFSPELEA
                     MRYRDQYNNWYDQKEDFGIDYTSFPKTFMLDNTKKENQIYWEYVLPLTESTKSWEDKR
                     LKNAYYMEVTLWKGEKSVKYVIDNIDITGNIYDLTYIQPV"
     gene            complement(4396..5544)
                     /gene="wecB"
                     /locus_tag="PK733_05595"
     CDS             complement(4396..5544)
                     /gene="wecB"
                     /locus_tag="PK733_05595"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ10051.1"
                     /translation="MQKLKVAIVFGTRPDAIKMAPLVKELKANENIETIVCVTAQHRQ
                     MLDQVLDIFNIVPDYDLNIMQSRQTLEHITTRSLEGLAEVFEETKPDVVLVHGDTTTC
                     FSSSLAAFYKQIKVGHVEAGLRTFDKYYPFPEEMNRRLTGVLADYHFSPTPSNKENLL
                     REGVNEEKIYVTGNTVIDALKTTVKKGYIFHDEKLQKVDFINKRIVTVEAHRRENLGS
                     PLENICEAVKEVADKYNDMEIVYPVHLNPAVREVVQRILGKHERIHLIDPIDVQDMHN
                     LMDRSYMIMTDSGGLQEEAPSLGKPVLVLRNETERPEAIKAGTVCLAGTDRQNIITLA
                     NKLLDNNDEYNKMARAVNPYGDGKASERIVKALLCEFGISNKRPEDFR"
     gene            complement(5600..6835)
                     /locus_tag="PK733_05600"
     CDS             complement(5600..6835)
                     /locus_tag="PK733_05600"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ10052.1"
                     /translation="MKIVYEYIISFAVAFIVSFSATPIAKKVAFKLKAIDIPKDNRRM
                     HKKPIALLGGLAIFCGFLLSVLFTVLSSKITGTQWLESFRLIAGLLAGASIITLTGII
                     DDIKPISAKMRLLLQFVAALAAIFISDIRIERITNPFSASGVTELNPYISYPLTIFWI
                     IGITNAFNLIDGLDGLAAGVSSISSLSLFFVSMLTPTVGPFSAIITAALAGATLGFLP
                     FNFNPAKIFMGSTGAYFLGFTLSVVSVEGMFKSYAAISIAIPILALGLPLFDTIFAII
                     RRLVNGKPIMSADRGHLHHRLIDMGLSQKQSVLVLYIASAALGLCAIVMADKGALCAI
                     ILLITVSIFVIAGAKYMVELTGKDDDADMDILIHDKENGPGKKASGSISNMGGVISGD
                     KGTARANVMMKALKKAGNR"
     gene            complement(7145..7612)
                     /locus_tag="PK733_05605"
     CDS             complement(7145..7612)
                     /locus_tag="PK733_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOJ10053.1"
                     /translation="MRPTWDEYFMEIVDLIKTRSTCIRRQVGALIVKDKRILATGYNG
                     APIGCKHCSQVGCLRQELGIPSGQRHELCRGIHAEQNAIIQAAYSGVSVKDGILYVTN
                     QPCVLCAKMAINAGIRKIVFKGDYPDELAMTMLRESGIRVVKFEPGLTFDQEE"
     gene            complement(7617..8060)
                     /gene="rpiB"
                     /locus_tag="PK733_05610"
     CDS             complement(7617..8060)
                     /gene="rpiB"
                     /locus_tag="PK733_05610"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ10054.1"
                     /translation="MIAIGNDHAGYKLKIEVIEHLIKKGYEVKDFGTDCESCSVDYPD
                     FGLAVAEAVKSGECEKGILICGTGIGISITANKVPGIRAALCTNSFMARMSIEHNDAN
                     ILALGSRVIGTGTALEIVDTWFETSFIGGKHARRIEKISQIENRG"
     gene            complement(8123..8662)
                     /locus_tag="PK733_05615"
     CDS             complement(8123..8662)
                     /locus_tag="PK733_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOJ10055.1"
                     /translation="MKKILFICTGNTCRSSMAEGFFNDAVGKDSALAESFISFSAGIM
                     AMDGDNASNNAIQAMREGWGIDISFHKAKSIRYEYIKNAYLILTMTQAHKDAILQLFP
                     EINSKVYTLKEYAAETIDSERQKDQLFKNYNFDFNYDFNIDIPDPYGKPLELYKYCAH
                     DIKKAVDLTIERLKLLTNL"
     gene            complement(8686..9759)
                     /locus_tag="PK733_05620"
     CDS             complement(8686..9759)
                     /locus_tag="PK733_05620"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOJ10056.1"
                     /translation="MKTEILKIDAHNICMDKLKYAADIIKNGGLVAFPTETVYGLGAN
                     ALQKGAIKKIFEAKGRPSDNPLIVHIADIAALEELAVEPPSVWQKLAGKFWPGPLTLI
                     FKKSPVIPSEITAGLDTVAVRMPAHPIALALIEESSLPIAAPSANSSGRPSPTLASHV
                     IEDLDGKIDLIIDGGSANIGLESTVLDITSNPPLILRPGGLTLEQLRIELPDVELDPA
                     ISSKDSMAGNNNLKEFIPKSPGLKYKHYSPKAQLIIVEGSMDKVVCRINELSKKMINE
                     GKKIGVLSTDHTKPLYKSSVVMSLGDRSSPDTIASNLFLSFREMDRQDVDVIFAEAID
                     KNGLGLAIMNRMKKAAGYNIIKV"
     gene            complement(9875..10639)
                     /locus_tag="PK733_05625"
     CDS             complement(9875..10639)
                     /locus_tag="PK733_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ10057.1"
                     /translation="MNHLLKITLVGLVSGIAGTSIGGLIGISLNKISDRLLGFVLEFS
                     AGLMTSVVCFELIPEALKLSGTFATFAGIFTGIIVILAIEAYINNSDFIKNRKKSYVK
                     NHKTKSSLLRAGILMSIGIALHNLPEGFAVGSGFEASLNLGTTITAVIIIHDIPEGIA
                     MALPMKTGGFSKTKTLIYTILSGVPMGIGALMGAVLGGLSDFYIGACLGFASGAMLYI
                     VFVNLIPESKKIYLGRLSSIGNLLGIICGIIVSIYN"
     gene            complement(10800..11885)
                     /gene="prfA"
                     /locus_tag="PK733_05630"
     CDS             complement(10800..11885)
                     /gene="prfA"
                     /locus_tag="PK733_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ10058.1"
                     /translation="MFENLQSAENRYEEINMKISDPLIISNQDEYRKLMKEHSDLEDI
                     VFKYREYKKVSEDFEEAKELLGQNLDKDFREMVHAEFNESKEKLEQIKEELRILLLPK
                     DPNDERNVIVEIRGGAGGEEAALFAGVLFRMYSRYAERSQWKCEILDSNPTELGGFKE
                     VVFSIEGKGAYSRLKYESGVHRVQRVPSTESGGRIHTSTTTVAVLPEVDDVEIDINPA
                     DLEIDTYRAGGAGGQHVNKTDSAIRITHIPTGIVVTCQDERSQHKNKDKAMKVLRSKL
                     YEMAQEEQDSEVAQSRKSQVGTGDRSERIRTYNYPQGRVTDHRIGLTLYKIDEILDGD
                     LDEVIEALITSDQSSKLGNGSFEDENY"
     gene            complement(11929..12531)
                     /gene="recR"
                     /locus_tag="PK733_05635"
     CDS             complement(11929..12531)
                     /gene="recR"
                     /locus_tag="PK733_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ10059.1"
                     /translation="MSYYAAPIAKLIEEFAKLPGIGQKTAQRLAFHVLNLSAEKAFNL
                     ADAIKEAKQKVKYCSKCGNLTDIDPCSICSSGHRDKSIICVVENPRDVIAMEKTREFK
                     GLYHVLHGAISPMEGIGPEDIKIKELLLRIQTGEIKEIILATNPNIEGEATAMYIAKL
                     LKPLGVRTTRIAHGIPVGGDLEYADEVTLARALEGRHEIW"
     gene            complement(12686..13030)
                     /locus_tag="PK733_05640"
     CDS             complement(12686..13030)
                     /locus_tag="PK733_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOJ10060.1"
                     /translation="MAKGGFPGFGGANMNNLMKQAQKMQKDMAKLQEELEEKTVEASA
                     GGGAITVVASGKKEILEINIKPEVIDPDDLEMLQDLIMAAVNEALRKAEEMVSSEMNK
                     LTGGLGGFPGLF"
     gene            complement(13311..15068)
                     /gene="dnaX"
                     /locus_tag="PK733_05645"
     CDS             complement(13311..15068)
                     /gene="dnaX"
                     /locus_tag="PK733_05645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ10061.1"
                     /translation="MSYIALYRKWRPLVFDDVVEQEHIVKSLKYSVRTGRIAHAYLFC
                     GTRGTGKTTMAHILSRAINCLNPHDGDPCNKCEICSGILSGSILDVMEIDAASNNSVD
                     NVREIRDEVIYTPSQAKYKVYIIDEVHMLSTGAFNALLKTLEEPPAHVVFILATTEPH
                     KLPATILSRCQRYDFRRITSESIAARLDKIAKANDIQVEKEALKLIARLSDGALRDAL
                     SILDQCISLGNKEITYKDVLSITGMVNESFISAFVDSLIKRYVNDTLNMVNTLIMEGK
                     DISQFVSELIVYYRNLLICKIIEDVSSVIETSGEVIETMKTQAVLLEKNEIINTIKEL
                     STLESSIKWSNHPRVMFEVTLIKILSGMFGSINADNTGNSGDSGNVGNIGNAGSIGNI
                     SGNIGNIADNDVFGRRVDYSDILKRLTALEEKISTSEMSSKPVRLDRPNTSEEVEENK
                     DVKRVTPRNVADSCKSLEDAWVKVLAELTNCGKKPLCSFLNNTRITALNKNCIGIVFS
                     DQSDVNKKIVSQTDNLQLIEKILNSILGKELRVKCVAESDMENSPNIEQEDSIVDDCT
                     DKIVRFANNLNVPINIYDK"
     gene            complement(15368..15632)
                     /gene="ffs"
                     /locus_tag="PK733_05650"
     ncRNA           complement(15368..15632)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK733_05650"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(15979..16128)
                     /locus_tag="PK733_05655"
     CDS             complement(15979..16128)
                     /locus_tag="PK733_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10062.1"
                     /translation="MTIKKKSIVTKLNNYDRKILNKYTRFYTNMIESNTASAPQLENQ
                     LLDGK"
     gene            16685..17560
                     /gene="galU"
                     /locus_tag="PK733_05660"
     CDS             16685..17560
                     /gene="galU"
                     /locus_tag="PK733_05660"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOJ10063.1"
                     /translation="MKVRKAVIPAAGLSTRLLPATKAQPKEMLPIVDKPAIQYIVEEA
                     INSGIEDILIVTGRGKRAIEDHFDKSYELEEELKKKGKHDLLAIVQDISNLVNIHYIR
                     QKEPKGLGHAIYCTKSFIEDEPFAVLLGDDIIDSEVPCLKQMIEVYDKYQTTIIGVQP
                     VPDNDVSKYGIVDCSREEERVYKVNDLVEKPEKEKAPSNIAILGRYIITPKIFEFLEK
                     AEPGKGGEIQLTDALRKLASVEPMYAYNFVGDRYDVGNKMGYLEAMVELALKRDDLKD
                     EFYAYLKDVIRKYNK"
     gene            complement(17817..18356)
                     /locus_tag="PK733_05665"
     CDS             complement(17817..18356)
                     /locus_tag="PK733_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ10064.1"
                     /translation="MSSKILFSSVNYDKYDANVTLPAKFSRMLDKSDIKDFVKGKLTA
                     VKMHLGRNIGYSTIHPLYVKILIDKLKEYGAKVYITDQVTEGARSRGYTEDFLGVPIV
                     DACGVTNKYYYEKQVDFKTFKNVDIAGNIHDAEAIIILSHTKGHLFERLHRKDPFIQL
                     RELEKRGLGTSDYEMEEVY"
     gene            18586..19491
                     /gene="cysK"
                     /locus_tag="PK733_05670"
     CDS             18586..19491
                     /gene="cysK"
                     /locus_tag="PK733_05670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ10065.1"
                     /translation="MKVTESVLDLIGNTPIIGLRKMTGLNIFAKAEFLNPGGSIKDRV
                     AKYMIEEAEKKGKLKPGMTIMEPTSGNTGIGIALEGVQKGYKVVIAMPENMSEERKKI
                     IAALGAECLLTPAEESIMGSVNEVKRRAESDPSIFVPQQFENPDNPKIHYLTTAQEIW
                     SQLDGKIDIFVSGLGSGGTLCGVGKFLKEKNPDIVIAAVEPKNVSALLGHEPGLHKIQ
                     GIGDGFIPPVLDTSLVDEVVEVSDDDAITTSRKLAKVEGLLVGTSSGANVWASVKMVE
                     KYGKDKIAVTVLADRAERYFSTALI"
     gene            complement(19922..22906)
                     /locus_tag="PK733_05675"
     CDS             complement(19922..22906)
                     /locus_tag="PK733_05675"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ10066.1"
                     /translation="MLTKNRFKNILNIIKLVSVYALVLALALCIYPNNTAIAQQDNSN
                     STQEIIHQSSNSYTLTSGAVYENIRRFTTEGWLNINVIRVDTTNPNVKLDALTNGESM
                     QKLMSVPSLTKSRNAAGAINGSFFNWSKTPGLNSPIGPIVESGKIITASSDFNRYGNS
                     MATFAIGEMNRVFCDYWKTDIRLISENGASIPVARYNVQLNSNGDITVVNRQWSQISI
                     GKPADPQQTVSSGSAEDSAGNTAGLAAGSASNTYSEIVEMVVKDNTVVEIRKNLPPVE
                     IPENGYIVIAANNNTKVIVDNFKEGDIVNLEIATTPDWNNISMAVTGGAVILKDGAIP
                     AKFSHDSPGRNPRTAIGSSKDGKQVIIVTVDGRQEKSLGMTLTELAKLMAELGAYNAI
                     NLDGGGSTTMTARKPGTNIINVVNSPSDGMPRSIANAIGVISTAPASPLAGLVIDTED
                     RNVFVNTSRSYTIRGYDNYYNPVEVNLEQVKWSVSGIDGVFQDNTFYPKSVGSGKIKA
                     TIGNISAEHEINSLSSPVELILSEKQIFLQTTGTKRFTVKGKNKNGYYAKINPEDITW
                     SVSGDIGSFKGDTFTAAKEGKGYIEASSGDTKAYCAVSVAADVVVVADNFEKINGTYL
                     SYPENIPGKYELSSEQKRSGNYSGKLTYDFNDSTGSRASYLLFSNGGYKLGNNVQKIG
                     IWVYNSNPNPNWLRALIYDSKGGRHYIDITQNMDWTGWKYVETSVAGISEKPLTLARI
                     YLVQTRDVPDAGHIYLDDLTFISIPDQKSGDISIPQDTIPADEANKAVSFQEGNNSFK
                     FIAFGQSSKAPVNMLQNLLLRRLATKINENAETAAFIGSSSYNFPKAISKSVICVDSA
                     LKAYDIKNSRLIQLDTRKKGLRVSHPAQWKWFLTQLDSFTGDNVFIFMTDHPQNFSDK
                     LETELFQDILTEYRQKTGRNIWVFYKSTINGSFMERGIKYISTAGYEIEDLNPSNANQ
                     VKYVHVTVQGKEVTFQFKPVVP"
     gene            complement(22950..>23244)
                     /locus_tag="PK733_05680"
     CDS             complement(22950..>23244)
                     /locus_tag="PK733_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOJ10067.1"
                     /translation="LSLFYEGRIHTFSPVTYLDRKDILVIRPLIYSEEKDIKGFINAN
                     NIQVAPSQCQISDTTKRHSMKNFLAEFQKDIPMMKDNIFGAIKRSGKDGWVDI"
BASE COUNT         7192 a         4459 c         4185 g         7408 t
ORIGIN      
        1 tggattatgg agagaataat actcaggaat atcttaatgg tattgtattt gtaccagtca
       61 gggtaacagg tacacttatg gatacaatat tttataataa tcctgaagaa agggaaaaga
      121 gatataacag ggatgatatt cacagttgga caatgagcct gaatgaaaca acttacccta
      181 atattgcccc tgatgattca ggtcctagtg gatcttacaa gaacaagaaa gatgcaataa
      241 taaccttgtg gtttaaacga agcgagatat tccagaggca aacatggaag cttaatgcat
      301 attcagaggc cctgttcctt gatggtaaat ccagtaagag aaatgcaacc ggcacactgc
      361 tgttcacaat taaagcagca cctcaaaacc aggaaccacc acaagagccg ccaccggaag
      421 caatagagga acctccgcca ccgcctgtta taatagagcc cgattgtcat ataccacatc
      481 ccggctttga tattgtacca taccctgctt atgataacac ggatttaagc caggttttat
      541 caaggaaggt atatataaat ggggatgaag tagatgatgt tgagttcttt agtgggaatt
      601 atatattcgg tgatggaaac gatggaataa agaagataga tgttatttat gaaagcatgg
      661 atgaaataac cagttcaact actcaatggg catacatata tgatacaaaa ccaacggcac
      721 agtataagct ggagggtgca tttaaggaga acagaaaact attggcttca gaaaattgtg
      781 catcagggaa cactcaaata gtccttgata aataccccat agtatcttat aactggaaat
      841 ttatatctat agacggagaa tcaagttcac ttaagcaaag aactccaaca tcagataaat
      901 atagggaatt tttgtataaa aagccgggag tatatagatt ggagttaact gtaacaaata
      961 cattaggcag agtatcaaag ccctatacac tggactttgt tatatacgaa gatacaccac
     1021 ctgcaataga aatgaatata tggaacggag tattaaccag gggagaagaa ttaaaactgc
     1081 actattcagc aagcagtgtt gataatgata gtatagcatt aaaaaccctg cagctatatt
     1141 atgacagcaa taatgacgga gaatgcgatc agcttgttaa aacatttcct gatggtgagt
     1201 ttgccggcta tgcacctgac aaactgggga aatataggct ggtggcaaca gcaactgaag
     1261 aatttgggca ggaaaccctg ccagaatata caactgttgc tgacaaaaag aagaaaaccg
     1321 cagaaagtga attctgggtg gacaaccttg tgccgatgac cgatatatac gtaaatgtac
     1381 ctgtagttaa gccggaaatt gatttgtatg tcatgatgga taaaaatcta aatgagagta
     1441 taaaacaata cataactgca aacagaatta actttaacaa ctttttaaga acaaagaata
     1501 tactgccaaa ggttgaaacc tgggatatga aaacctatga atactctcag tcggtaagtg
     1561 atacatacag atccggggaa tcatatccac catcctccat attgtattca agtggcgggt
     1621 attcgggaac actttatctt gagcctgaca gtacagtgga caatggagaa gacagagatt
     1681 ttggcagctg ggatacaaag acggaatcaa aggaatttac aaggtctcac agcaatacaa
     1741 taactgaaga atggtgggat ggaagtctat acagaacaac tgaatccgat ccggaacctt
     1801 cgagttatga tgtagatgag gacggctaca gcggaagtat cccgcaagtg agcagggacg
     1861 gcccttacaa ccggaaggta gataactatt attcagatag agaatttata atacatcatt
     1921 ccaatacggt tactgactgc tacaacgatg ggaaactggt atactcctct aattctgatc
     1981 ctgcaccttc aagctattat ataaatgagg atggctacag tggatatatt tacagggatg
     2041 acaccgaagg tccgtataac gaaaaacact ggttcgaagg tcaatatgaa tactatgaaa
     2101 aaacatgggt agctgtattt agaggtactc taagcaagat tcatgcatgg gatatccatg
     2161 aaacatatat aagtacctat agagggacat tgactaaatc aaagcaggta tgggttcctg
     2221 atatgagatg ggtaagcgat tacataggct attactcagg catagtgtac aaatatgtac
     2281 ggcaaccata ttcaaatcct ttcagagcca catcagacaa atatgttgtg tatataagtg
     2341 atggaaacat aaatgagctt acggaccttg gaatggtaat gtcaaaggca gatgcccggc
     2401 tgatacttgt gggagccaac tctattagga atcagatggc gcatgatcat tatatatcaa
     2461 gagataaacc catggagcag attatgcaag aggtgttaga ttacatagca ataaacagtc
     2521 ctgctataga aaagcattat gtgcttgcag gagtagacat ttttgatata aaagctattg
     2581 attttgatga tgaaaatgat ccaattattg agagaaagtt tcagtacgtg caagatgcaa
     2641 attattttga taatccaaca ggaatggaaa attttgcagc agctagcttt tctgaaaata
     2701 gcaattgggt agataccaag gtaggcaagt ttaatgaagt aggagagttc cgtatattca
     2761 gaagaataaa ggatcagcct tcaccagatc ctaactttgc aaactactca tatttttctg
     2821 gaactcctga gttaagagta tatgctcaca gaaaacctat tgcaaaagct tcattggaat
     2881 gggattatga caattctaaa ggagtttata aaaccaggtg ggtatgtcaa tcctatgatt
     2941 ttgaccacca gtattccagg gaggataagg gaattgcgga aaggaaaata atgttcagaa
     3001 ggaccggcgg cgaatggtat tattatgttc cggaagaatt atcatctggg acttatgaat
     3061 tgcaatacta tgtcaaggat attgaaggag catggtcgga tccgtttgca atgaatttta
     3121 cattgctatc agcaccatcc atccagtttg atgcaaagct gtgtaccaaa gattcaaaat
     3181 tcagcctcaa taatatccct gcttccgaag acctggaggt ttacgatgca tggacaaggt
     3241 acccttataa tgtgaagctg gaaatggcac tttataatgg aagtataagg ataagccctg
     3301 taaaatcagt gaactatagt tcttcaacag gtgtaaaaac aggcaatgat atacaatgga
     3361 atagtataag ctatcaagta ccgaatacca taaaagacgg aacatatacc tttaaagtag
     3421 aggctgttga tactaacaat tcaagcatac aggcagtgaa aagtttttct gtaatggtaa
     3481 acacccctat aaatctggta ccgtcaatgc ctgaaactgt tatgacagga aatacagcag
     3541 atataaaggc tgctactgag aaatatgcaa gtgttacaag agttgccatg ttttatggaa
     3601 catcatatga gcagaatctg gagctttcaa gcgttctcca agaggatatg aaggaatgga
     3661 gcaacaaata tacaatacct tccaatatac cggaaggtgt atacacagca cgatttatat
     3721 cgacaacgca aaacggcaag tctgaaatga aggatgctac atttaaagta gaaactttaa
     3781 aaattagcaa agtaaccata gaggggtatt ggaaccattg gagaggtcag gtagatatgt
     3841 ttggcaggca acttgcaaat gagcctcata ggttcttaag cctggaaaga gtaaaaatta
     3901 atgtgcagac agatggcttt gcagataaag ttgaaatacg gttcagtcct gaattggaag
     3961 caatgagata tagagaccaa tataataact ggtatgatca gaaagaggat tttggaatag
     4021 actacacatc ctttcccaag acattcatgc ttgataatac taaaaaagaa aatcagattt
     4081 attgggaata tgtactgccg ctgacggaga gtacaaaatc atgggaagat aaacggctta
     4141 aaaatgcgta ttacatggaa gtaactcttt ggaagggtga aaagtcggta aaatacgtaa
     4201 tagataatat agacataaca gggaatatat atgatttaac ctatatacaa cctgtatagt
     4261 agtttattat ccaaacaaac tggacaaact ggacaaaccg gcaaaagctg cttaaatctg
     4321 ttaaaattat ctaatgatgt ttaaaatggt aaaaagctgt ttaataaaac aaacagcttt
     4381 ttaccatata atttattatc taaaatcctc cggcctttta ttgctaattc caaactcaca
     4441 tagtaatgcc ttcacgattc tttctgaagc ctttccgtcc ccatacgggt ttactgctct
     4501 ggccatttta ttatattcgt cgttattatc aagtagttta ttggcaagag ttattatatt
     4561 ttgcctgtca gtgcctgcca aacatactgt tccggctttt atggcttcag gtctttctgt
     4621 ttcatttcta agaacaagga caggtttccc cagagacgga gcttcttcct ggagtcctcc
     4681 ggagtccgtc ataatcatat aagacctgtc cataaggttg tgcatatcct gtacatcaat
     4741 agggtctata agatgtatcc tttcatgctt tcccagaatt ctttgcacaa cttcccggac
     4801 agccgggtta aggtgtacgg ggtatacgat ttccatatca ttatatttgt cggcaacttc
     4861 tttgacggct tcgcaaatgt tctccagggg agagcccaga ttctcccttc tgtgtgcttc
     4921 cacagttact attcttttat taatgaaatc aaccttctgc agcttttcgt catggaatat
     4981 gtagcctttt ttaactgtgg tttttaatgc atctataact gtattgcccg ttacatatat
     5041 cttttcttca tttacgcctt ccctcagtaa attttcctta ttggaaggag taggtgaaaa
     5101 gtgatagtcg gcgagtacac cagtcaatct cctgttcatt tcttcaggga agggatagta
     5161 tttgtcaaag gtccttagac cggcttcaac atgtccaacc tttatctgtt tgtaaaatgc
     5221 agcgaggctt gacgaaaaac aggttgtagt atcaccgtga accagcacta catccggttt
     5281 tgtttcttca aaaacctcag caaggccttc aagtgatctt gttgtgatgt gttccaatgt
     5341 ctgtctgctt tgcattatat taaggtcata atcaggtact atattaaata tgtccaggac
     5401 ctgatccagc atttgcctgt gctgtgcggt aacacatact atagtctcaa tattttcgtt
     5461 tgccttcaat tcctttacca gaggagccat ttttatagca tccggtctcg taccaaaaac
     5521 aattgctact tttagctttt gcattatatt catcctttcc ggtatagcaa caggcacagt
     5581 tgaatctatg agctttatgc tatcggttgc ctgctttctt taaagccttc atcataacat
     5641 ttgcccgggc agtaccttta tctccactaa tcactccacc catattgctt atactgcctg
     5701 aagctttttt acctggtcca ttttctttat cgtgtataag aatatccata tctgcatcat
     5761 cgtctttacc agttaattcc accatgtatt tggctcctgc tataacgaat attgatacgg
     5821 taattaatag tataattgca cataatgcgc ccttatcagc cattactatt gcacatagac
     5881 ctaaagcagc actggcgatg tataggacaa gaactgattg tttttggctt aatcccatat
     5941 caatcaatct atggtgcagg tgacccctgt cagctgacat gataggtttt ccatttacca
     6001 atcttctgat aattgcaaaa attgtatcaa aaagcggaag tcctaaagcc agtataggaa
     6061 ttgcaataga aatagcggca taagatttga acataccctc tacagatacc actgacaagg
     6121 taaatcccag gaaatatgct cctgtgcttc ccataaatat cttagcagga ttaaaattga
     6181 agggaaggaa ccccagggtt gcacctgcca gagcggcagt aattattgca gaaaatggtc
     6241 caactgtagg tgttaacatt gatacaaaaa acaaagacag ggatgatatt gatgaaacac
     6301 ctgctgcaag accatcaagt ccatcaatca gattaaatgc attagttata cctattatcc
     6361 agaatatggt aaggggataa gatatatatg gattaagctc tgtaacaccg ctggctgaaa
     6421 atggattggt aatacgttca atacgtatat cagatataaa tattgcagcc agagcggcaa
     6481 caaattgcaa taataaacgc attttcgcac ttataggttt tatatcatct ataatcccgg
     6541 ttaatgttat aattgaggcg cctgccagca gtcctgcaat tagtctgaag gattccagcc
     6601 attgagtgcc tgtaattttt gagctaagta ctgtaaataa tacagaaagg agaaaaccac
     6661 aaaaaatagc caatcctccc aataaagcaa taggtttttt atgcattctc ctattatcct
     6721 ttggtatatc tatagctttc aatttaaatg caactttttt tgctataggt gttgcagaaa
     6781 atgaaacaat aaacgctaca gcaaaagata ttatatattc ataaactatt ttcatgtaag
     6841 tcacaccttc ttttgtaaaa taggaaaagt tcaatttaac cttagtataa ggcttgtttt
     6901 aaacctattc agcaattcat accctggcta aagccgacaa gtaagatagg ggttttcttg
     6961 cgtcatacta agcaataaat tatataatat attgcttaac atattataca tcatacaaca
     7021 tacataatat gttaaggaaa tattaaaatt attatatact ataaaattat tatatatata
     7081 agtattaccg tactaaatat taaatactca ttgcagctaa ttataaatat accaaatatc
     7141 caaactattc ttcctgatca aaagttaacc ccggctcgaa cttcacaact cttattcctg
     7201 attctcttag catggtcatt gcaagctcat caggataatc acctttaaaa actatctttc
     7261 gtattcctgc attaattgcc atttttgcac ataaaacaca aggttggttt gtaacgtaca
     7321 ggataccgtc ctttacacta acccccgagt atgccgcttg gattattgcg ttttgctctg
     7381 catgtatacc cctgcacagt tcgtgccttt gacctgatgg gataccaagt tcctgtctca
     7441 ggcagccgac ttgtgaacaa tgtttacaac ctataggggc accattataa cctgtagcca
     7501 atatacgctt gtcttttaca attaatgctc cgacctgtct tctaatacat gttgacctcg
     7561 ttttaataag atcaactatt tccataaaat attcgtccca tgttggtctc aaaaaattac
     7621 ccccgatttt ctatttgcga tattttctct atacgtcttg catgttttcc accgataaaa
     7681 ctggtctcaa accaggtatc cacaatttcc agtgctgtgc ctgtacctat aacccggctt
     7741 cctagagcaa ggatgtttgc atcattatgt tctatgctca ttctggccat aaaactgttt
     7801 gtacagagag cagccctgat tccaggaact ttattggctg taattgagat tcctatgcct
     7861 gttccgcaga taagaatacc cttctcgcat tcaccgcttt ttacagcttc agctacagca
     7921 agtccaaaat ccggataatc aacagaacag ctttcacaat cggttccaaa gtctttaact
     7981 tcatatcctt tttttataag atgttcaatt acttctattt ttaatttata acctgcatga
     8041 tcatttccta tcgcaatcaa ttacaacaac ctccaattcc atattttata gcattttata
     8101 ccttaagtca aagaaagttt tgttacaaat ttgttaataa ttttagtctt tcgattgtta
     8161 aatctacagc tttcttaata tcatgagcgc agtatttata caattctaat ggtttgccgt
     8221 atgggtcggg aatgtcaata ttgaaatcgt agttaaagtc aaaattatag ttcttaaaca
     8281 gctgatcttt ttgtctttca ctatcaatag tttctgcagc atattccttt aatgtatata
     8341 ctttagaatt tatttcaggg aataactgaa gtattgcatc tttgtgtgcc tgagtcatcg
     8401 ttaatatcaa atatgcgttt ttaatatatt catatctaat tgatttagct ttatgaaaac
     8461 tgatatctat tccccaacct tctctcatgg cctgaattgc attattgctt gcattatctc
     8521 catccatagc cattattcct gcagagaagg aaatgaatga ttctgccagg gcggagtctt
     8581 ttccaacagc atcattaaaa aagccttcag ccatactgct cctgcaagtg tttccagtac
     8641 aaatgaacag gatttttttc aagaaaacct caccttcttt caatactaaa ctttaattat
     8701 attataccct gctgcttttt tcattctatt cattatagcc aatccaagac catttttatc
     8761 tatagcttcc gcaaatatta catccacatc ttggcgatcc atttctctga aactgaggaa
     8821 taagttggat gctatagtat caggagaact cctatctccc agtgacataa ctacagagct
     8881 tttataaagt ggttttgtat gatctgttga gagaacacca atctttttcc cttcattgat
     8941 catcttttta cttaattcgt ttattctgca tactacttta tccatagaac cctctactat
     9001 tattaattgg gcttttggtg agtagtgctt atattttaaa ccaggagatt ttggaataaa
     9061 ctctttcaaa ttattattcc cggccatgct gtcttttgat gaaatagccg ggtcaagttc
     9121 tacgtcgggc aattcaattc tcagttgttc cagggtaagt ccccccggcc tgagtatcaa
     9181 tggtggatta gatgtaatat cgagaactgt tgattctaaa cctatgtttg cacttccgcc
     9241 atcaattatt aaatctattt tcccatctaa atcttcaatt acatgacttg ccagtgttgg
     9301 gcttggacga cctgaagagt ttgcactagg tgcagctatg ggaagactgg attcttctat
     9361 taaagccagg gcaatagggt gagcgggcat ccttacagca acagtatcca atcctgcggt
     9421 aatttcagaa gggatgacag gagacttttt aaatataagg gtaaggggcc caggccaaaa
     9481 tttacctgcc agcttttgcc aaactgaggg aggttcaaca gccagttcct ctaaagcagc
     9541 tatatcagct atatgaacaa taagtgggtt atctgatggt ctgcctttag cttcaaaaat
     9601 tttttttata gctccctttt gtaatgcatt tgctcccagt ccatatactg tctcagtagg
     9661 aaaagctacc aagccgccgt tttttataat atcggcggca tatttaagct tgtccataca
     9721 tatgttatgt gcatctattt tcaatatttc tgttttcaat ttaccttatg ctccttttgt
     9781 ttacgtagaa tcttatactg caaaagtata aacaaacaat atgctatcaa aaattatcct
     9841 gaaaggcata actttccaaa ttggcatagt aatactaatt ataaattgat actattatac
     9901 cacaaattat acccaatagg ttacctatag atgataagcg tcccaaatat atttttttgg
     9961 attcgggtat aaggtttaca aagacaatat agagcattgc tcctgatgcg aaaccgaggc
    10021 atgctcctat ataaaaatca gacaggccgc ctaaaacagc gcccattaga gcaccaattc
    10081 ccattggcac tcctgataat atggtatata ttaaagtttt tgttttagaa aagccgcctg
    10141 ttttcatagg cagtgccatt gcaattcctt caggtatatc gtgtatgatt ataactgcag
    10201 tgatagttgt tccaaggttt agtgaagctt caaaccccga acctacggca aatccttcag
    10261 gcaggttatg taaggctatt cctattgaca ttaaaattcc tgcccgcaat aagctgcttt
    10321 ttgttttatg attttttaca taactttttt tacggttttt aataaaatca gaattgttta
    10381 tatatgcctc aatagccaga attacgatta tacctgtaaa tataccggca aaagtggcga
    10441 aggtaccact aagtttcagg gcttcaggta taagttcaaa acaaactaca gaagtcatga
    10501 gtcctgcaga aaattctaaa acaaatccca gcagacggtc gcttattttg ttaagcgaaa
    10561 tcccaattaa accgcctatg ctggtgccgg cgattcctga aacaagtcct acaagggtta
    10621 tttttagcag atgattcata tttacctcac gcatacttat atatagaaca ctcatataca
    10681 aaacactcat atatggatta ctaatatatg aatattcata tattagacaa agatgtctat
    10741 attatttgta tgcgctaata tatcaaaaca accctattct atcgtaaaag cacataaatt
    10801 taatagttct catcttcaaa gctcccattt cccagttttg aagattggtc acttgttatt
    10861 aatgcttcaa taacttcgtc cagatctcca tccagtattt catcaatttt atataaggta
    10921 agaccgattc tatggtcagt aactctgcct tgaggataat tatatgtacg tattctttcg
    10981 ctgcgatcac cggtgccaac ctggcttttc ctggattgag caacttcaga atcctgttct
    11041 tcctgggcca tttcataaag ttttgaccgt aatactttca tagccttatc tttgttttta
    11101 tgctgagatc tttcatcctg acaggtaaca actatacctg taggtatatg agttattctt
    11161 atagctgaat ccgtcttatt tacgtgctga ccgcctgcac ctcctgcacg gtaggtatca
    11221 atttccaagt ctgcaggatt gatgtcaatt tctacatcat ctacttcagg aagtacagca
    11281 actgtagtag ttgatgtgtg tattctgcct cccgactcag tgcttggtac tctttgtact
    11341 cggtgaacac cgctttcata ctttaatcta ctgtaggcac cttttccttc aatagagaaa
    11401 accacttcct taaaaccacc aagctctgtt gggtttgaat ccaagatttc acatttccat
    11461 tggctgcgtt ctgcatatct ggaatacatc ctgaacagaa caccggcaaa caaggcagct
    11521 tcctcgccac cggcacctcc tcttatttca acaataacgt tacgctcatc attgggatct
    11581 tttggaagca gaagtatcct aagttcctct ttaatttgct ccaatttttc cttggactcg
    11641 ttgaattcag catggaccat ttcacgaaaa tccttatcaa ggttttgccc cagcagttcc
    11701 tttgcctctt cgaagtcttc gctgactttt ttatattccc ggtacttaaa aacaatatct
    11761 tcaaggtccg aatgctcttt cataagtttc cggtactcat cctgattgct tattattaat
    11821 ggatcactaa ttttcatatt tatttcttca tatctattct ctgcgctttg caaattctcg
    11881 aacatatttt acacctcaaa attgtcttca ttaaatagtc tcattgaatc accagatttc
    11941 atgacgcccc tcaagagcac gggcaagtgt tacttcgtct gcgtattcaa ggtctcctcc
    12001 tacaggaatt ccatgagcta tgcgtgtagt tcttacgcct aaaggtttta agagctttgc
    12061 aatatacatt gcagtagcct cgccttcaat gtttgggttg gtagcaagga taatttcttt
    12121 tatctcacct gtttgtatcc gcaaaagaag ctctttgatt tttatgtctt caggacctat
    12181 gccttccata ggggagatag caccatgtaa aacatgatac agtcccttaa attcccgggt
    12241 tttttccatg gcaataacat ctcttggatt ttctactacg caaatgatag atttatctct
    12301 gtgccccgag ctgcatatgc tgcagggatc aatatctgtt aaattaccgc atttggagca
    12361 atattttact ttttgctttg cttcttttat tgcatcagcc aggttaaagg ctttttcagc
    12421 agataaatta agcacatgaa acgccagtct ctgtgcagtt ttttgaccta tgcccggtaa
    12481 ttttgcaaat tcttctatta gctttgctat aggtgctgca taataactca tttatatatc
    12541 atccttgtaa ttattcagaa tcattcctgc tattctatat aatttacgat ttgaagtaat
    12601 caatgaagaa ttagcaaatc gtaagttaca tcaaggtaac agaagcaggt tttaaacatt
    12661 ttaaaacctg ttctatatag ctgttctaga acaatcccgg gaaaccaccc aatcctcctg
    12721 ttagtttatt catttcgctg ctcaccattt cttcagcttt tctcaaagct tcattaacag
    12781 cggccattat aagatcctga agcatctcca aatcgtcagg atcaattact tcaggcttga
    12841 tatttatctc aagaatttcc tttttaccgg aagctacaac tgttattgct ccaccaccgg
    12901 cacttgcctc aacagttttt tcctcaagtt cttcctgaag tttggccata tccttttgca
    12961 tcttttgtgc ctgcttcata agattattca tatttgcccc tccaaatccg gggaatcctc
    13021 cttttgccat taaccattcc tcctgtttcc tgtttatttc acatatcatg acgcaatata
    13081 cacatgcata tcataaatat aactatttaa tgttaatgca gtgggtaatt aactgctaca
    13141 attatacagc aaaacatact gtataatcaa ctataagaca ttgatttgca tacaaatatt
    13201 aatattaatc ctgctttgtt atgttgccaa tggagagcga catcttcccg atgctatcaa
    13261 aaattatccc ggaagacatc gcttttcaaa ttagcatagt agtattaatt ctatttatca
    13321 tatatattaa taggtacatt aagattgttt gcaaaacgta caattttatc tgtacaatca
    13381 tctacaatag aatcttcctg ttcaatattg ggggagtttt ccatatcact ctctgccaca
    13441 cattttactc ttaactcttt tcccaatata gagttgagta ttttttctat caattgcaga
    13501 ttatctgtct gggaaacgat ttttttattt acatcgcttt gatctgaaaa aactatccca
    13561 atgcagttct tatttaatgc agtaatcctg gtattattta aaaatgaaca taatggtttt
    13621 ttcccacagt ttgtcaattc tgcaagaacc tttacccatg cgtcttccag gcttttacac
    13681 gaatctgcta cattacgggg agtcactctt ttaacgtctt tattttcttc aacttcttct
    13741 gaagtattag gtctgtcaag tctaacaggc tttgaggaca tttcggaggt acttattttt
    13801 tcttccagag ctgtaagtct tttaagtata tctgaataat caactcttct gccaaaaaca
    13861 tcgttatcgg cgatattgcc aatattgccg cttatattac ctatactacc tgcattacct
    13921 atgttaccta cattacctga atcacccgaa ttaccagtgt tatctgcatt aatgcttccg
    13981 aacattccac ttaaaatttt aatcagagtg acttcaaaca taaccctggg gtggtttgac
    14041 cacttaattg aagattcaag agttgataat tccttgatag tattaataat ctcatttttt
    14101 tccagcaaca cagcctgtgt cttcatggtt tctataactt ccccggaagt ctctataaca
    14161 cttgatacat cctcaataat cttgcatatt agaagattcc ggtaatacac tattagttcg
    14221 gatacaaact gagagatatc ttttccctcc ataataagtg tattaaccat attaagggtg
    14281 tcgttaacgt atcttttgat taatgaatca acaaaagctg aaataaaact ttcgttaacc
    14341 attccggtta tagaaagcac atccttatag gttatttcct tatttcctaa agaaatgcat
    14401 tggtccagaa tgctgagtgc atcacgcaaa gccccgtccg atagcctggc aataagcttc
    14461 aaggcttctt tttccacttg aatgtcattt gccttagcaa tcttatcaag gcgtgctgca
    14521 atgctttcag aagtaattct tctaaaatca tatctctgac atctggataa aattgtcgca
    14581 gggagcttat gaggctctgt agttgcaaga ataaatacta catgagccgg tggttcttcc
    14641 agtgttttta ataaagcatt aaatgctcca gtagaaagca tatgtacttc atcaataatg
    14701 tataccttat attttgcttg agagggggta taaatgacct catcccttat ttctcttaca
    14761 ttatctacac tattattaga agcagcatca atttccatta catcaagaat actgcctgat
    14821 aatatgccgc tgcatatttc acacttatta caagggtctc catcatgggg gtttaagcaa
    14881 ttaatggctc ttgatagtat atgagccatg gtagttttac cagtgcccct tgtaccgcag
    14941 aaaaggtaag catgagcaat acgtcctgta cgcacactgt atttaagcga ttttacaata
    15001 tgttcctgtt ccactacatc atcgaagacc aaaggcctcc atttacggta aagcgctata
    15061 tatgacataa ttatctccca ccttatacag cacgtcataa ttataacaac attcaatgtt
    15121 gttataatta tgacacaaaa actcattcag cgtcaacata ttgagattca aagcacttaa
    15181 tcaaaatgct ccgattttca taaaaaaaag tcagctttgc tatttttagg tcaaagtatt
    15241 cccaaccagt aaatgcgtta aggtagactt tcttgaaact catttaacgg gaagtgcaca
    15301 ttaaggtaat ttatacttcc ctattcgtta taaaaaaaga cccatatggt cttttaaaat
    15361 taattggcca tgcacctgtc tttgacttat ccctacaagc gttaccaata cagttggctc
    15421 aacacaggcg accttacggc acacaaaagg gcttgcttac cgctgcttcc ttccgaacct
    15481 gacggggttc acaagttttt attgcgtaag acccgcatct catcaccact tataaaggtc
    15541 agccctcaca ggaaaaaagc ctgggactgg aattcaatcc tgctatagcg ggttgcaggt
    15601 tcagggcacc gctacctccc catctagcat ggccaaatat taacttgtca aaataaatat
    15661 aacaaacaac aaacttatta tacttgataa taattaatat ttcaacatgt tttgagaaaa
    15721 tggatataat ttccacaaaa aattcctaaa aaaaagcaaa aaaagaagca ctttcttata
    15781 atttataaat aattcctaat tacatagtgc ttcaccaaat aaactcaacc aaatcaactc
    15841 aaataatcta tattaaagga ggaacaaaaa tgaaaaattt agtattattg gctgtggtat
    15901 ttatattata ccataatttt ctttggggta aagttaaatt tatatgacaa aatttaataa
    15961 atgatgaaca gctagcatct attttccatc aagcaattga ttctccaatt gaggtgctga
    16021 agctgtatta ctttcaatca tatttgtgta gaaacgagtg tatttgttga gaatcttcct
    16081 gtcataatta ttaagttttg taacaatact ctttttttta atagtcataa tatcacatcc
    16141 ttttatccag gtgctgacag tatattggta gttcattaat tttacagcga aaaacagtga
    16201 aaccaatatt atatcaatat tattatgtcc aaaaagtcat taaaaaatca gattcattaa
    16261 aaattgggtg catttaaaat ttttgaagaa atatattcca gaagctatca ttacagtact
    16321 tagagccttc actaatgcct cttccaataa tttcttcaaa aataaaatat gcacccctaa
    16381 aatttcaggt tggatagaat ttcgaggttt gatcttatat atttataaaa ctatacgata
    16441 ctatattttg tattatagga aaaatatttc catgtgctgt gcgaacactt gacagtttct
    16501 ataaagccac atattattaa tgtgagaaaa gcataggttg ttactataat ttgcaacaat
    16561 tcagcttacg actatacagg cgagagactt attggtgctc ttgccaagga tttgccataa
    16621 aaggcagaat tcttgtgtaa agtacaggtg atacttgtaa atgcgtaaaa agggggttaa
    16681 gaaattgaag gtacgtaaag ctgtaatacc tgcagccgga ctttccacaa ggttgcttcc
    16741 tgcaacaaag gcacagccaa aagaaatgct tcccatagtg gataagcctg ctattcagta
    16801 catagtagaa gaggccataa attcgggaat agaggacatt ctaattgtta ccggacgcgg
    16861 gaaacgagct attgaggacc attttgacaa atcttatgaa cttgaggaag agcttaagaa
    16921 gaaaggtaaa cacgatttgt tggctatagt tcaggatata tcaaaccttg tgaatataca
    16981 ttatataaga caaaaagaac ctaaagggct tggccatgct atttactgta ccaaatcctt
    17041 tattgaagat gaaccttttg cggtgctttt gggggacgat ataattgatt ccgaagtacc
    17101 atgtttaaag caaatgatag aagtctatga taagtatcaa acaacgatta ttggtgtgca
    17161 gcctgtccct gataatgatg tttcaaaata tgggattgtt gattgcagca gggaagaaga
    17221 gcgggtatat aaggttaatg atctggtaga aaaacctgaa aaggagaagg ctccatcaaa
    17281 tattgccatc ctgggcagat atataattac tcctaaaata tttgagttcc ttgaaaaagc
    17341 agaacccgga aaaggtgggg agattcagct tacggatgct ttaaggaaac ttgcctctgt
    17401 agaaccaatg tatgcttaca attttgttgg agatagatat gatgtgggca acaaaatggg
    17461 atacttggaa gcaatggttg agcttgcctt gaagagagat gatttgaagg atgaatttta
    17521 cgcttattta aaagatgtaa tacgcaagta caataaataa gggaatgatt acccaagtca
    17581 ccggtgactt gaaaattgtt tattactaaa tactactttg ttatgttgcc aatggaaagc
    17641 gcatttctgg agggtaattt ttgctgaccc gtaaggaata cacctgaggg aagggtcgca
    17701 agaccttcta tagagacatt tccccatgcg gcatcttcct gatgctatca aaaattatcc
    17761 tggaagatat cgcttcccca attggcataa tagcattaat acacttagtc tattacttaa
    17821 tacacttctt ccatctcata atccgatgtt cccaatcctc ttttctcaag ttccctaagc
    17881 tggataaagg gatcttttct gtgaagcctt tcaaacagat gaccttttgt atgggagagg
    17941 attattatgg cttcagcatc atgaatattg ccggcaatat ctacattttt aaaagtttta
    18001 aagtcaacct gtttttcata atagtattta ttcgtaacac cgcatgcatc aactatgggt
    18061 actccaagga aatcttcggt ataacccctt gatcttgcac cttcagttac ctggtcggta
    18121 atataaactt ttgccccata ttcttttagc ttgtcgatca gtattttaac gtaaagaggg
    18181 tgaattgtag aatatccgat attacgacca aggtgcatct taaccgcagt caacttacct
    18241 ttaacaaagt ctttaatatc tgacttatca agcatcctgc taaatttagc aggtagtgtt
    18301 acatttgcat catatttatc ataatttact gatgagaaca aaattttaga actcatactg
    18361 ttttcctcct atgacgtaat aaagtgttga cttattgaca attatatcat taacagtata
    18421 ctatgaaaag aggtttagag gtaattcagg acaggaaatt ttgtaaaaga ttaagtaccg
    18481 ggtagagcac tgaaacctta cgaaattaac cattttgaat tgaatgagta tttgtactaa
    18541 aattaatctt aatatataag tttagcttcg gagggatatt ttgttatgaa agttactgaa
    18601 tcggttttgg atttaatagg gaatacgcct attattggac ttagaaagat gacaggtttg
    18661 aatatttttg cgaaagcaga gtttttaaac cctggcggca gtataaagga cagggttgca
    18721 aaatacatga tagaagaagc ggagaagaaa ggcaaattaa aacctggtat gacaataatg
    18781 gagcctacat ccggcaacac cggaataggt attgctcttg aaggtgtaca aaaggggtat
    18841 aaagttgtaa ttgctatgcc tgagaacatg agtgaggaga gaaagaaaat aattgcggct
    18901 cttggtgctg aatgtctgct tactcccgct gaagaaagta ttatgggttc tgttaatgaa
    18961 gtgaaaagaa gagctgaatc agatccgtcc atattcgtgc cacagcaatt tgaaaatcct
    19021 gataacccca aaattcatta tcttactact gcgcaggaaa tatggagcca actggatggg
    19081 aaaattgaca tatttgtttc aggtttgggt agtggcggga ctttatgcgg tgtgggaaag
    19141 tttcttaagg agaagaatcc tgatattgtt atagctgctg ttgaacctaa gaatgtatca
    19201 gctttgctgg gacatgaacc ggggcttcat aaaatccagg gtataggaga cggttttatc
    19261 ccgccggttc ttgatacatc tcttgtagat gaagtggttg aagtttctga tgatgatgct
    19321 ataaccacat caagaaagct tgcaaaggtt gaaggacttc ttgtgggaac atcttccgga
    19381 gccaatgtat gggcatcagt gaaaatggta gagaagtatg gtaaggataa aattgcagta
    19441 acggttttgg cagacagggc ggaaaggtat ttcagcacgg cactgatatg atacgttact
    19501 agtttgaggg tgcatttaaa atttttgaag aaatatattc cagaagctat cgttacggta
    19561 ctaagagcct ttaaattctg ctacgggcaa aaccaaactc acaaaataat ctgaataaaa
    19621 gtttcataat tattttgttc aaacactgat tttgctcttc cttcgctccg attaaaaggc
    19681 tttatagtac cttcactaat gcttcttcca ataatttctt caaaaataaa atatgcaccc
    19741 ttagtatgat acagccttga caaacttttt ggggacactt ctcaatataa aagaaatacc
    19801 cgttactaga gagaagtgtc ccctgatctt atatacttgt acttgtgatc aatacttgct
    19861 acatcaatac ttatacttgg gaattttgaa atcagtgttt gtcatccaac tttatagcac
    19921 tttatggtac gacgggctta aattggaagg ttacctcttt accctgcact gttacatgta
    19981 catatttcac ctggtttgca ttggaggggt taaggtcttc tatttcataa ccagcagttg
    20041 atatatactt aatacctcgt tccataaaac ttccgtttat agtgctttta taaaataccc
    20101 atatatttct tccggttttt tgtctatact ctgtaagtat atcctggaaa agctcagtct
    20161 caagtttatc actgaaattt tgaggatgat ccgtcatgaa gataaataca ttatcacctg
    20221 taaatgagtc aagttgggtt aagaaccatt tccactgagc ggggtgactg actctcaatc
    20281 cttttttacg agtatccagc tgaattaaac ggctgttttt aatatcgtat gctttaagtg
    20341 cactatctac acaaattaca gatttgctaa ttgctttagg gaaattatag ctgctgctgc
    20401 cgataaaagc agcagtttct gcattttcgt taatttttgt tgcaagcctt ctcagaagca
    20461 ggttttgaag catattaacc ggtgcttttg atgactgccc aaaagctata aatttgaaag
    20521 aattatttcc ttcctggaaa gatactgctt tattagcttc atcggcaggt attgtatctt
    20581 gaggtatact aatatctccg gatttttggt ccgggatcga aataaaagta agatcatcaa
    20641 gataaatatg tcctgcatca gggacatccc ttgtttgaac caggtatatt cttgctagag
    20701 ttaaaggttt ttcactaatt ccggctactg aagtttctac atacttccat ccggtccagt
    20761 ccatattttg agttatatca atgtagtgcc tgccgccctt tgagtcatat attaaggctc
    20821 tgagccagtt tggatttgga ttgctgttat aaacccaaat acctattttt tgaacgttgt
    20881 taccaagctt atagccccca tttgaaaaaa gcagatatga agccctggaa cctgtagaat
    20941 cgttaaaatc ataggtcagc ttcccagaat agtttcctga acgcttctgt tccgatgaca
    21001 gttcatattt cccgggaata ttttctgggt atgacaaata tgttccatta atcttttcaa
    21061 agttgtcagc aaccacaact acatcagctg caactgaaac agcacagtat gcttttgtat
    21121 cacctgagga ggcttcaata tatcctttgc cttctttagc tgcagtaaag gtatcccctt
    21181 taaatgagcc aatatcgcct gaaaccgacc atgtaatatc ttcagggttt atttttgcat
    21241 aataaccgtt tttgttttta ccctttactg taaagcgttt agtaccggtt gtctgcaaaa
    21301 atatttgttt ttcactaaga atgagttcta ccggcgaact tagcgaatta atttcatgct
    21361 cggcagatat attacctata gtagctttga ttttccccga acctacagat ttgggataaa
    21421 atgtattatc ctgaaatact ccatcaatac ccgaaacact ccattttacc tgttccaggt
    21481 taacttcaac aggattgtaa tagttatcgt atcctcttat ggtataagac cttgatgtgt
    21541 tgacaaacac atttctatct tctgtatcaa taaccaatcc tgcaagcggg gatgccggag
    21601 ctgtggaaat aacacctatg gcgttggcta tgctcctggg cataccatct gaaggactgt
    21661 taaccacatt aataatgttt gtgcccggtt tcctggctgt cattgttgta gatccgccac
    21721 catcaaggtt tattgcatta taggcaccaa gttcagccat aagcttggct agttcggtaa
    21781 gagtcattcc caggcttttt tcctgcctgc catccactgt tactattatt acctgctttc
    21841 catcttttga actgccaata gctgttcttg gatttcttcc aggactgtcg tgggagaatt
    21901 ttgcaggtat agcaccatct tttaatatta ctgctccacc agtgactgcc atggatatat
    21961 tattccaatc aggggttgta gctatttcca gattaacaat atcgccttct ttaaaattat
    22021 caactattac tttagtatta ttgtttgcag caataacaat atacccattt tcaggtattt
    22081 caactggtgg aaggttttta cgtatttcaa ctactgtgtt gtctttaaca accatttcaa
    22141 caatttcgga gtatgtattt gatgcactgc ctgcagccag gcctgctgtg tttcctgccg
    22201 agtcttctgc tgagcctgat gaaacagttt gctgcggatc cgcgggtttt cctatggata
    22261 tctgggacca ttgccggtta actactgtaa tatcaccatt gctattgagt tgtacattgt
    22321 atcttgcaac agggattgaa gctccatttt cagatattag ccgtatatcc gttttccagt
    22381 aatcacaaaa tacccggttc atttcgccta tggcaaaggt tgccatagaa ttaccatacc
    22441 tattgaaatc actggaagca gttataattt taccggattc tactattggg ccaatggggc
    22501 tgtttaaacc gggagttttt gaccaattga agaagctgcc atttattgca cctgcagcat
    22561 tccttgattt agtaagggaa ggtactgaca tcagtttttg cattgactca ccatttgtta
    22621 aggcatcaag ttttacattg gggttggtgg tatcaactct gataacattg atgtttagcc
    22681 atccttcggt agtaaatctg cgtatattct catatactgc tcctgaagta agggtatatg
    22741 agttcgatga ctgatgaata atttcttgtg tagaattcga attatcctgc tgggctatag
    22801 ctgtgttatt tgggtaaata cataaagcta gggctaaaac aagggcatat actgaaacta
    22861 gtttgattat atttaaaata tttttaaatc tatttttagt taacattgac atctccatcc
    22921 tttattgaca aaatatatct tgctggtaat tatatatcaa cccagccatc tttacccgat
    22981 cttttaatag caccaaaaat gttatctttc atcataggga tgtctttttg aaactctgcc
    23041 agaaaatttt tcattgagtg acgttttgta gtgtcgctaa tttggcattg actgggcgca
    23101 acttgaatat tattagcatt tataaagcct tttatatctt tctcttccga atagattagc
    23161 ggcctaatta ctaaaatatc tttacggtca agataggtaa ctggtgagaa tgtatgtatc
    23221 ctgccttcat aaaacaaact taac
//
LOCUS       DAOSDD010000042        23232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_973_length_23232_cov_5.299823, whole genome shotgun sequence.
ACCESSION   DAOSDD010000042 DAOSDD010000000
VERSION     DAOSDD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23232
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_23232_cov_5.299823"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..737
                     /locus_tag="PK733_05685"
     CDS             294..737
                     /locus_tag="PK733_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOJ10068.1"
                     /translation="MLKIYFDACCYNRPFDDLTQERIRLETEAILLVMNKISNNGYKL
                     YGSPAIEFELSRMNNDYKKKMVIMLYSSMKKELLKYDEKINIRSKALDAFNIKYFDAL
                     HIAFCEFYNIDYMLTTDKVLINAVKRSDIAIRVVNPIEFVMEVLE"
     gene            734..937
                     /locus_tag="PK733_05690"
     CDS             734..937
                     /locus_tag="PK733_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10069.1"
                     /translation="MINALNLKDLGNLQEEGLKALSEKLGPIGMVNFIRLFDNGKGDY
                     SKERRENLNSITEVDFKDFLKNQ"
     gene            1478..2056
                     /locus_tag="PK733_05695"
     CDS             1478..2056
                     /locus_tag="PK733_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10070.1"
                     /translation="MKNAYIEFPRDWRIINSYIFAMIDDRDHLNKNISEIRRLCRKTL
                     NECTDQLFRDNAISNMIRICYDSEVEEWYSKVSIDLKGFLKAERYRYREQWDLYNKQN
                     DLNQYVKLIVFLLNLRPVNCSASESLDLCRVNGLEKCQKANVQNLNFLKHISIFEKCS
                     CYSSQNQCDFIISDKPQYESEEIFTRGYRYGA"
     gene            2190..2456
                     /locus_tag="PK733_05700"
     CDS             2190..2456
                     /locus_tag="PK733_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10071.1"
                     /translation="MKWSEVRKLYPDKFIKFEVINYDTMDNKRYVKDIAVIDVIDDSK
                     IAMKEFSQCKEGQFVYSTKNEQIVIEVVKYIGIRRSPQDVPAKV"
     gene            2437..2823
                     /locus_tag="PK733_05705"
     CDS             2437..2823
                     /locus_tag="PK733_05705"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOJ10072.1"
                     /translation="MYQLKYKHGLLYADITLKHANKEIYITDVIIDTGASHTVILPDF
                     LTDNDLGFDNSDELVVMSGIGGAEASAIRKRIDSISIGNISMKDIIIDFGVVDPKDRI
                     NGLIGLDFLKTAKITIDLDEVAIYKK"
     gene            2844..3014
                     /locus_tag="PK733_05710"
     CDS             2844..3014
                     /locus_tag="PK733_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10073.1"
                     /translation="MVVCWFEVHKDYMEILNIAVTESVHHHVIGGKMVATLWKNYGVA
                     IEAKTDDDAVDF"
     gene            3557..3742
                     /locus_tag="PK733_05715"
     CDS             3557..3742
                     /locus_tag="PK733_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10074.1"
                     /translation="MENKVARLVRIENQVDINKDSQIEFLFQYQKIVLLALAEQWIIN
                     DLQCQQCIDKLTAQFRK"
     gene            3912..5606
                     /locus_tag="PK733_05720"
     CDS             3912..5606
                     /locus_tag="PK733_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ10075.1"
                     /translation="MKVAAYCRVSTDKEDQANSFESQQRYFKEYIERQPDWELFDIYA
                     DEGTSGTSTKKRAAFNRMINDANLGRFDMIITKEVSRFSRNILDTISYTRDLRRKGIG
                     VLFLNDGINTFEPDSELRLSIMGCIAQEESRKTSNRVKWGQTRQMEKGVVFGRSMLGY
                     DVKNGVMIINPEGAEIVKLIFHKYVNELKGTSVIARELREVGYKTLTGNVFWTNTAIL
                     KIIRNEKYCGDLCQKKTYTPDYLTHDKKYNHGAEEKVYIHNHHEPIIDRELWDRAQKE
                     IKRRDVDSSVKEGHGNRYPLSGKIKCAECGAAFVARYKKRKDGNKYKYWRCGTAAAHG
                     RKKKDKAGNEVGCDIGRQIRDEMCMNILKQTVSSLFVDKKAIIENITSIVADVIRDSE
                     SSEQLSLEKLQKQMNDLTEKKKSVMDAFFSQDITKDEMRMMNEKYDADISLTADKISA
                     VKKKQSLSYSCQDIKSDVRKSVENIVDCSVKQDNFYGSLLDKMVVYDDNRVEVLLNLL
                     PTKWMYVIENLKDIQKSTGERKNASCDYNRGEVHCDTSVPISLRVARACGVTIENR"
     gene            complement(5546..5737)
                     /locus_tag="PK733_05725"
                     /pseudo
     CDS             complement(5546..5737)
                     /locus_tag="PK733_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
     gene            complement(5741..6022)
                     /locus_tag="PK733_05730"
     CDS             complement(5741..6022)
                     /locus_tag="PK733_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOJ10076.1"
                     /translation="MEMNKQSLLKKIMELDFVAVDLQLYLNTHPCDKEALCKFNTIVN
                     QAHMLRQEYERLYGPLCSYRSPSRYPWQWINNPWPWNYCFNFRMSREEL"
     gene            complement(6009..6305)
                     /locus_tag="PK733_05735"
     CDS             complement(6009..6305)
                     /locus_tag="PK733_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOJ10077.1"
                     /translation="MKMQEWTTGNIPGIPSVDESSPYHGGMYQQYQCIPQELVIRNVK
                     LARAYVPFQKLCSLYPPEIALHKGTVFPELYSPYMVKKEQKIVPIREECGDGNE"
     gene            6722..7549
                     /locus_tag="PK733_05740"
     CDS             6722..7549
                     /locus_tag="PK733_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ10078.1"
                     /translation="MKNIFEVAVCQMLVTESKERNIDNAVEMLEIAAENGAQLALLPE
                     MFICPYNSSLFSLYAENMESSKTLSAISNIAKKHNMYIAAGSIPEKYEDHLFNTCFVF
                     DAKGNIIGRHRKTHLFDIDIPGKITFKESDSLSAGDNITVVDTEYCKIAVAICYDIRF
                     PEFIRMAALKGAKLLVVPAAFNMTTGPAHWELLMRTRALDNQLYIAAASPARDETASY
                     IAYGNSMIVDPWGMVLSRAGTGKEIIYMDIDLDEVEKIRMELPLLKHRRTDIYSFNL"
     gene            7700..7963
                     /gene="rpsO"
                     /locus_tag="PK733_05745"
     CDS             7700..7963
                     /gene="rpsO"
                     /locus_tag="PK733_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ10079.1"
                     /translation="MQKEAKQNIISMYKLHENDTGSPEVQIALLTERINHLNEHLKIH
                     KKDHHSRRGLLKMVGQRRGLLNYLEKIDVERYRAIIEKLNLRK"
     gene            8552..10675
                     /locus_tag="PK733_05750"
     CDS             8552..10675
                     /locus_tag="PK733_05750"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ10080.1"
                     /translation="MHKIFKMELAGRELVIETGKYAQLANGAVLVRYGDTVVISTATA
                     STEPREGIDFFPLSVDYDERLYAVGKIPGGFIKREGKPSEKAILTSRVIDRPIRPLFP
                     KDLRNDVSVINTVLSVDQDNSPEIASMIGTSAALSISDIPFKGPIGGVVLGLVDDKVV
                     INPNAKEREKSQMYVTLAGTKDKITMIEAGANEIADDVMLNAIKTGHEEIKKIVEFID
                     EIVAQEGKPKFEYKSFEVPDEIFNAIKDYAYNRMKEALLSADKQIREGNVAELTKEII
                     EHFANVYPDMETVIAEAVYKMEKKAVRELILKEGKRVDGRHLDQIRPLYAEVGILPRT
                     HGSGLFQRGQTQVLTTVTLGALGEVQILDGVDLEEEKRYMHHYNFPSYSVGEAKPSRG
                     PGRREIGHGALAERALESVIPNEQEFPYAIRLVSEVLMSNGSTSQGSVCGSTLALMDA
                     GVPIKRPVAGISSGLVIDEENPDNFIVFMDIQGIEDFFGDMDFKVAGTKEGITAIQMD
                     IKVEGLTEEIIRQAFEMTKKGRYQILDDVILKTIPEPRKELSKYAPKILTTNIDPDKI
                     RDVIGPGGKVINKIIADTGVKIDIEDDGRVFVSTSDTESGMAAIKIIEGIGKEVEPGQ
                     VFLGKVARIMPFGAFVEIAPGKEGLVHISKLDKKRVNKVEDIVNIGDEILVKVIEIDK
                     QGRINLSRKDALMDEEKQTNNNDVE"
     gene            10748..12019
                     /locus_tag="PK733_05755"
     CDS             10748..12019
                     /locus_tag="PK733_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ10081.1"
                     /translation="MVKKYVLDNGIRIVCEQIPYLRSVSIGIWVGAGSRNESKLNNGV
                     SHFIEHMLFKGTENRTAKDIAVSIDSIGGQLNAFTGKEYTCYYAKTLDTHIDIAVDVL
                     SDMFFNSRFDNKDISVEKQVIIEEINMYEDTPEELVHDVLAEIMWDGDPLGYSILGTY
                     ESLDAIDREKIKSYLTGNYHPNNTVIAIAGNYDEDKMLDLITKYFNRWKNEEYNTTDY
                     SPVTYKSGVKLRNKDTEQIHLCVGFNGIENGSDDIYPLLVVNNVLGGGMSSRLFQKIR
                     EEEGLVYSIYSYPSSFKNSGAVTIYAGMNPGQLEQVSRLIMLEIKQLKNRGISESELE
                     KSKEQLKGNYMLGLESTNSRMTSIGKSELVLGYVNTPDEILEKIERVNNDMVNNIINK
                     VFDVEKACVAAVGKIKENIDIASLINIDTKI"
     gene            12212..13084
                     /gene="dpsA"
                     /locus_tag="PK733_05760"
     CDS             12212..13084
                     /gene="dpsA"
                     /locus_tag="PK733_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HOJ10082.1"
                     /translation="MEKKSFAIIGGDLRSLKLIDLMEQDGNVVKVHGFDNYKNIANME
                     INNDLHKTIEGTDVVIGPLPCSNDNETINAPFCSNKILINDIFKIMGKNQLFVAGRIS
                     SKIAHLAEVYNVYTVDILDREEMAVLNAIPTAEGAIQIAMEEMQITLHDSNVLVMGFG
                     RVGEVLAKMLNGIGANTFVEARKYADFAWIKSYGYKPVAINQLDEYISDMDVIFNTIP
                     ALILDEKLLGKVRKDCLIIDLASKPGGVDFDKAKMMDLKVNWALSLPGKVAPITAAKF
                     IKDTIYNIIEELGV"
     gene            13088..13666
                     /locus_tag="PK733_05765"
     CDS             13088..13666
                     /locus_tag="PK733_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HOJ10083.1"
                     /translation="MMLKGLKIGLAITGSFCKVDDVFPEVENMVKEGAVVFPIISDSI
                     DKYDTRFGRAEDTKIKLEGLTGNKIISTIVTAEPIGPKSILDILVIAPCTGNTLGKLA
                     NGITDTAVTMAAKAHLRNQKPIVIAISTNDGLGANAKNLGLLINTKNMYFVPFGQDDP
                     VKKCNSLVAKMDMIIPTVVEALDGKQIQPVLV"
     gene            13945..18333
                     /locus_tag="PK733_05770"
     CDS             13945..18333
                     /locus_tag="PK733_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10084.1"
                     /translation="MTQFDLIGSVVKLRKFAPVVDLQWAESLRDEGSLNLIDSYIVTD
                     ELAGHFEKVFESFTLKRHERKALREGSILDASVHQRAHILRGQYGTGKSYFLLMVSAL
                     LESLGNDDLFNDLYNKFHVFDGIRFHMENLKQRNAKYMVVRIDGVKNADMHFNELVQK
                     KVVSRMQEILGETDFYDSYSIAVRKLEEYRRDPIFSKLLEDALVSRSLSYNSLIESLK
                     ASKRQSLKKYREIIESITRHKINEGFDSLEEFLQSASSYVKNKGYDGVAILFDEFSAY
                     INASIEDGRITTDLAAVQSLAQLTIPREGLDLFFVCSMHIDIGMVLGNVMATAEEIQK
                     VRGRFAEMTLSFNNSANLVENILIVDRNKFSNLQAKYREYFGALPSRYPDMAKVYPIH
                     PHTVNSIISVSSKFAQNERTIFSFFAETVNRKLREPVIKDERLNLITTGDIYNYFIDT
                     ISERNAILKDSALRCMSFCRTELEKDVIKALVIAHISAGEGSDSRLSSKDIAFILGVD
                     DIKQIDIFLKEMSANPASNIVFYEKEYRFEFIATGSTVADFTAALDKDALEIEPYGAL
                     LELIEEYGPSICIRKIYNVNPSRDLLPVRKDLRGVIYRPADLLKAIEQEIDNIDKDGR
                     IIFVIPGFNDKMEKDFEEAVKAMLFKAPANICVAVPKSFPYGLEKDIRFFTAARNMEK
                     SGSVDENGRKTLRKILQPIEKIVDYEIKKLGNIFNFTFIFSDNIVKEKFTALEDFYKF
                     LLKRHYSKFPRVDAEAIRGKNSIHMMVENFFMFGEKTNIPANYSSELDKLIMEVLQPL
                     DMVKVERSGSGYSARLKIPEEENNRESFEIWNIVNDTSRPVREIFSFLEKAPYGLPDY
                     MTELYVAAAVAANQLAITYRGQVMQLNKMSIALLNSAGYALEKVRTAPLELKTEVKKV
                     WSVFCKINGRCVARNFEPEVPQSDEDVHSLIASDMSDTKIILEGLEGRLENAGAKNST
                     LFELMKALYDLRTLKNPVNYMEIFIQMPRKVFGINNDFTAFECFDSFFVFLAELSTSL
                     DSIRRVDAKLSSMRNLESIQEDGETSETTLYESTYGANNIAGLRTAYFEIIGDFQQLK
                     KDISNNVFLPEVLKELEGKLYKLIEDYNKVFVELHLEVADKAGKIHTLLDSSAAKLIE
                     AFESISFRNIKRVSDLRNELRGIKACSHKLTEKEHQPDQIGLVDRKNQVDQINRINRM
                     DRMDEMDRMDRIYQIDNEPLNCSCTGFVSGLAGLLEQIQLVQRMEQSIWKQISNIGEN
                     HILRILQLDEKVEERNMTLSDYLRKVGDTGADIMYTSAAGTGKANTASMSGTLGIPSI
                     FAADISADAACKDMYKDISEDTSKNEPDNYMLLQDWEKLKKYLKLGFETISGSSNSEE
                     VINLLGCLSKCINGYLIDSIAVKKQPEVEVKVKKKIGFRALYTQIQSEIANSGYKSVT
                     VDEFARTLQRIIDKVRKEYDEVNIEE"
     gene            18420..21272
                     /locus_tag="PK733_05775"
     CDS             18420..21272
                     /locus_tag="PK733_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ10085.1"
                     /translation="MENENILSLFKKDYETLMDSSKYIAKSDYNDIKARYNEYADSID
                     IYSRLEFDIDLHNERFVKAKLAEYKEYFDNLLSCIDSSIVLDEEQRRAILVDEDYCLV
                     IAGAGAGKTTTMAAKVKYLVEKLKINPEDIILISFTNKAVDELKERIIDKLGIPARVT
                     TFHAFGYDILRKIKEQPPGVNINAYNIIFNLFENTIYKNNSLLKKLILFFGYYFDIHE
                     DALSFESLEQFHAYKVEQEYETLRSSLGEYNVNVIDRRSKQVRTIRGEYLRSVQEVQI
                     ANFLYLNSIDYEYEKPYLYPVKTSRKPYTPDFWVSQGDNTCYIEHFGITEDGKSSIYN
                     PKQLSRYIEGIEFKKQLHAKNQTPLITTYSAYRDGKQLLKHLEEELIKEGFVLKPRDP
                     GEVYQKLANSGKDKYIVRFILLMIEFIRAFKTTGYDRGTFLWLKKRTDNVRTQLFLSI
                     AEEIYDMYQEELEKNNSIDFEDMINEAEKTLQEMQETGTNISCKYIIIDEYQDIARQR
                     FNLTKRLADITGAKVIAVGDDWQSIFAFSGSDITLFTKFLKLMGYGRELQITHTYRNS
                     QELIDIAGGFVQKNPSQIRKRLISSKHIEKPVILKTYDDTKELRTNRINAVVEAIGEI
                     MAQSNVTQPKITLSNMNQPNLNQEKAAQTNVTQRNMTQAKVTQTNVAQRNMTRLNETL
                     TNITKSNITQSDGKISILLIGRYNFDEYYLLKSESFIQLPNGKLKCNKYPQADITYMT
                     AHSSKGLGYDYVILINALEGKYGFPSQIEDDPVMKLVTVEDRSMSFAEERRLFYVALT
                     RTKNRIYIVTPESKPSRFVQELIRDWGVERPPKLKMNAYNNENINNITDSKSNRCPIC
                     EFPLTHKYNKNIGLELWICTNEPEICDFMTNDKSTIGDIFKCPACMDGYMIVKKNKDN
                     DNKFFGCTNHRPDGAGCNHIEYISEISNIKVKKLFV"
     gene            21312..21494
                     /locus_tag="PK733_05780"
                     /pseudo
     CDS             21312..21494
                     /locus_tag="PK733_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance protein"
     gene            21809..22507
                     /locus_tag="PK733_05785"
     CDS             21809..22507
                     /locus_tag="PK733_05785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ10086.1"
                     /translation="MRIIVLHGQAHKGSTYNITRLFLDNLSDESTEITEFYMPKDAPS
                     FCIGCFNCFTKGEEQCPHSDIVQPIAKAIEEADLLILESPCYVYGMTGQLKTFLDHLA
                     YRWMPHRPHPKMFSKVGLVISSAAGAGTKKVTKALQDNLFYWGVPKIYSYGKNVGASS
                     WETVKLEKKLKIEKEVIQIAAQILKCVGKTKPGIKTKVMFQIMRMSQKANNWNPTDKE
                     YWIKNGWLDKSKAW"
     gene            22611..22892
                     /locus_tag="PK733_05790"
     CDS             22611..22892
                     /locus_tag="PK733_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ10087.1"
                     /translation="MCNNINNNDSSNNNYDLTSNIIIKKDMERFKKQLDFLIEIDKLK
                     QVFRNTILMDASRLENDAEHMWHMAVGAMLFSEYSNNKDIHMLKVLKWY"
     gene            22883..23101
                     /locus_tag="PK733_05795"
     CDS             22883..23101
                     /locus_tag="PK733_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ10088.1"
                     /translation="MVLIHDVIEIYAGDTFAYYEQRHVDKEEREQNAANKIFALLPRE
                     QVRWFEKDFMDNLKEELPVPKKPFIYPC"
BASE COUNT         8229 a         3490 c         4792 g         6721 t
ORIGIN      
        1 taagatttac ctgataaaac tattatagca aggaagtgat gcaaataaaa gggttaatta
       61 aataaaaaga aaaaaatgta tatcttattt aacatttttg gctataaaca gggaaatgct
      121 aaatgccata ttttggacta aatgtaattc ccaacccaag ggtaaaaaat agtatataat
      181 tttttactcc tcagagctgt acatatttaa atcaagttga taaatatgta tccgtttgct
      241 atacttataa tatagatata aaaagtttta taaaaataag gatgatacta taaatgctta
      301 aaatatattt tgatgcttgt tgttataatc gtccatttga cgatttaacc caggagagga
      361 taagacttga aaccgaagct atattattgg ttatgaacaa aatatcaaat aatgggtata
      421 agctttatgg aagtccagcc atagaatttg agctaagtag aatgaataac gactataaga
      481 agaaaatggt tataatgcta tacagttcaa tgaaaaagga attgttaaaa tacgatgaaa
      541 aaattaatat tcgatcgaaa gcacttgatg catttaatat taaatatttt gatgctttgc
      601 acattgcttt ttgcgaattt tataatattg attatatgct tacaacagat aaagtgttga
      661 taaatgcagt taaacggtca gatatagcaa taagagttgt gaatcctata gaatttgtaa
      721 tggaggtgct tgaatgatta acgcgcttaa tttgaaagat ttaggaaatt tgcaggagga
      781 agggttaaaa gctctttctg agaagttagg tcccatagga atggtaaact tcattcgttt
      841 atttgacaat ggtaagggtg actattcaaa agaacgacgt gaaaacctga actctattac
      901 agaagttgac tttaaagatt ttcttaaaaa tcagtagaag ctcaaggata ttgttcttac
      961 aacttaaaat actaaaagaa ccatatctgt cattgtaagc tggttactgt attgcaacta
     1021 ttaaggctta tattatatta cttgagacta tctctagaca gattttttta atctcacatc
     1081 tgtacatgtt aacctgaatt ggtagctcgg tatcattgaa aatgaaatcc tatcgtgaaa
     1141 atattagcct atcagatata acgaaccagg ctgataagga acgtattatt gtcgcttgcc
     1201 agaaaagtgg tgttgatcag attatcaata aactaaacaa tggtcttgat agttttctta
     1261 cacgcaattt tgaattggtg ttacatttca gactgtatcg aaatgggaaa atgaaaattt
     1321 ttatcccgac atagaactat tgccgttcat tgcttcctat tttaatatat cagttgatga
     1381 tttaatagga tacaatcagg cattatcgga gaaaaaaatc gaagattatc ttaataagct
     1441 tgatagtgta accactgttt aggaagcagt tttattaatg aaaaatgcct atatcgagtt
     1501 tccaagagat tggagaatta taaactccta tatattcgca atgattgatg atagggatca
     1561 cctaaataag aacattagcg aaatcagacg tctttgtcgc aaaactttga atgaatgcac
     1621 cgaccaactt tttcgtgata atgccataag caatatgatt cgtatttgct acgattctga
     1681 agtcgaagaa tggtacagca aagtgtcaat tgatttaaag gggtttttaa aagcagagcg
     1741 atacaggtat cgtgagcaat gggatttata taacaaacaa aatgatttga accagtatgt
     1801 taaattaatc gtatttcttt taaatctacg accagtgaac tgtagtgcaa gcgaaagcct
     1861 tgatttatgc agggttaacg gcttggaaaa gtgccaaaag gcgaatgtgc aaaatctgaa
     1921 ttttctaaag catatcagca tttttgagaa gtgcagctgc tacagctcgc aaaatcaatg
     1981 tgattttatt atatcggaca aacctcagta tgaatctgaa gaaatattta ccaggggcta
     2041 ccgttacggt gcttagagct tccacttatg ccccttacca atattttctt cagacaaatt
     2101 aaatgcaccc taaattatgg atatgtttga tggtcttact tctttatgat ataatcaata
     2161 caggcttatt aaagcaggag gtaaaataaa tgaaatggtc agaggttaga aaactttacc
     2221 cggacaagtt cattaaattt gaagtcatta attatgacac catggacaac aagagatatg
     2281 ttaaagacat tgctgttatt gatgtaattg atgacagtaa gattgcaatg aaggaattct
     2341 ctcagtgcaa agaaggacaa tttgtttata gtactaagaa tgaacaaatt gttattgagg
     2401 ttgtaaaata cattggtatt cgaaggagcc ctcaggatgt accagctaaa gtataaacat
     2461 ggtttgctgt atgcagatat tactcttaaa catgcaaata aagaaattta tatcactgat
     2521 gtgattatag atactggcgc cagtcatacc gttattctac cagatttctt aactgataat
     2581 gacctgggat tcgataatag tgatgaactg gttgtaatgt ctggaatagg tggtgcagaa
     2641 gcgtcagcta taagaaagag aatagattcc atatcaatag gcaatatcag catgaaagac
     2701 attattattg acttcggagt tgttgacccc aaggatagaa ttaatggttt aattggtttg
     2761 gactttttaa aaacagcaaa aattactatt gatttggacg aagttgcaat ctacaagaaa
     2821 taatgtgtga aggacgtaat gcattggtag tttgctggtt tgaggttcac aaggattata
     2881 tggaaatcct caatatcgcc gtcaccgaaa gcgttcatca tcatgtcatc ggtggtaaaa
     2941 tggtagccac attgtggaag aattacggag tggcgattga agctaaaacc gatgacgatg
     3001 cagtggattt ttgaagaagt attaaaaaaa cattagagtg atatatcctg catagaaaca
     3061 ttgtgaggct aatgccccac ttagcgatta actctctctg agcaaaatcc cgaaaggcta
     3121 attaataggc tttccgggat ttttacgttc taatatcata aattatatac cttcattatc
     3181 taataacttg cttctatatt tttttatatc tcagattaac atttataagt gaaatattaa
     3241 atttatatta catttttcaa aaaggagctt accttttgcc caaaaatctg ctgattagta
     3301 gagggatatg atacttgcac acagtcgagg ttaaatccca gagtgtgccc aagtcgttga
     3361 cggagaggtg taattcctat gaagacgtga aactaccgtt tgccgaataa atacaataga
     3421 agctaaaaca gttaatttat actctgtatt aaaaacaact acagagttat gggcagacaa
     3481 gcaacaactt atttcagaat gaataatgtg taataacaga ctgcgaaata acaagctatg
     3541 ctagaaggtg agcagaatgg aaaataaagt tgcaagactt gtaagaattg agaaccaggt
     3601 tgatatcaac aaagattctc aaattgaatt tttgtttcag taccaaaaaa ttgttctgct
     3661 cgccttagca gagcagtgga ttattaacga tttacagtgt cagcagtgta ttgataagct
     3721 taccgcacaa tttagaaagt aatttctgcc gacacatggg gacagttcct ctgtattccc
     3781 tgcggtcact ggctttgcca gcgatacaaa cgaaccgcct ccaagtctca gttgtgtttt
     3841 cggaatccgc aaattcgtaa tacattatcg gcagtggtaa aataacgagg aaaggagatg
     3901 atgcgctttt gatgaaggtt gcagcatatt gccgtgtttc caccgataag gaggatcaag
     3961 caaattcttt tgaaagccag cagcgttatt ttaaggagta catagagcgt caacccgatt
     4021 gggagttgtt tgatatctat gccgatgagg gtacttcagg aacctcaacc aaaaaacgtg
     4081 ccgcctttaa tcgcatgata aacgatgcta atcttggccg ttttgatatg attattacaa
     4141 aagaggtcag tcgtttttcg aggaatattc ttgatacaat atcctataca agagatttac
     4201 gtcggaaagg cattggcgtt ttattcttaa acgacggtat caatacattt gagcctgatt
     4261 cggagcttcg actgtccatt atgggctgta tcgctcagga ggaaagcagg aaaaccagca
     4321 accgtgtgaa atggggacag acgcggcaga tggagaaggg tgtggtattc ggaagaagca
     4381 tgcttggtta tgatgtgaag aacggcgtta tgatcatcaa tcctgaaggt gctgaaattg
     4441 tcaagctgat ttttcataag tatgtaaatg agctgaaagg aacctcagta attgctagag
     4501 aacttcggga agtaggctac aaaaccttga ctgggaatgt gttctggaca aatactgcaa
     4561 tacttaagat catccggaac gaaaaatatt gtggtgacct ttgtcagaaa aagacttata
     4621 cccccgatta tcttacccac gataaaaaat acaatcatgg tgcggaagaa aaggtttaca
     4681 tccataacca tcatgagccg attattgacc gtgagctgtg ggacagagca caaaaggaga
     4741 ttaaacgtcg agatgtcgac agcagtgtta aagaaggaca tggaaaccgg tacccgctgt
     4801 ctgggaagat aaaatgtgca gaatgcggtg ctgcctttgt tgcacgctac aaaaagcgta
     4861 aagatggcaa taaatataaa tactggcgct gtggtacggc agccgcccac ggcagaaaga
     4921 aaaaagacaa ggcaggcaat gaagtcggct gtgatattgg caggcaaatt agggatgaaa
     4981 tgtgcatgaa cattttaaaa cagactgtct catcgctatt tgttgataaa aaagctatca
     5041 ttgaaaatat cacaagtatt gtagccgatg ttattcgtga tagtgagagc agtgagcaat
     5101 taagtcttga aaaacttcaa aaacaaatga atgacttaac agagaagaaa aaaagtgtga
     5161 tggatgcttt cttttctcag gatattacca aggatgaaat gcggatgatg aatgaaaaat
     5221 atgatgccga tatttcgctc actgcggata aaatcagtgc tgtaaagaaa aagcagagtc
     5281 tttcctattc atgtcaggat ataaagtcgg atgtgagaaa atcagtagaa aacattgttg
     5341 attgttcggt caaacaggat aacttttatg gcagcctcct tgacaaaatg gttgtttacg
     5401 atgataatcg ggtcgaggta ttgctaaatc tgttgccgac aaagtggatg tatgtaattg
     5461 agaacctgaa agacattcaa aagagcacag gagaaagaaa aaacgcttcc tgcgattata
     5521 acagaggaga ggtacattgt gacacttctg tacctatatc gttaagagta gctcgtgctt
     5581 gcggtgtaac cattgaaaac cgttgactca gatatctaag cgaagctgca agctcaccat
     5641 ccggtcctcc gtattgggta ataatatatt tagctaactt gggatttggt ttttttattt
     5701 taacaggata ctctaatttt ttctcataaa cccacataaa ctacaactcc tctcttgaca
     5761 ttctgaagtt aaaacaatag ttccagggcc atggattatt gatccattgc cacggatatc
     5821 tgcttggaga cctgtatgaa cataaaggtc catatagtct ttcatactcc tgccttaaca
     5881 tatgagcctg gtttacaatt gtgttgaact tacatagagc ttctttatcg caaggatgag
     5941 tattaagata taactgcagg tcaacagcaa caaaatcaag ttccattatt ttttttaaaa
     6001 gtgattgctt attcatttcc atcaccacat tcctccctga ttggaactat tttttgttct
     6061 ttctttacca tataggggct atataattca gggaaaaccg tccctttgtg aagtgcaatt
     6121 tcaggtggat aaagagagca aagtttttga aaagggacat aagctcttgc cagcttaaca
     6181 ttcctaataa ccaattcctg tggaatacat tgatactgct ggtacatacc accatggtat
     6241 ggactgcttt catctacaga aggaatcccc ggaatattac ctgtagtcca ttcctgcatt
     6301 ttcattctcg aaccaacctc cttacaaagt atttaatcta gacaaataat catttatata
     6361 aacataatat tcatcttatg taaatttgtt aacattaatt aattattcta tataatttac
     6421 gatttgaaaa aactaattaa gaatttgcaa atcgtaagtg gcatcaaggt agcagaagta
     6481 ggttttaaat gttttaaaac ctgttctata tagtaatttt gaattgtaat tataggcatc
     6541 ataaattatt gcataaacat gactgtttga tgtatattat tagaaaatga gtaggatgaa
     6601 gagatatatc tttgattgag atgaaggcgc atgtcttcaa aatccattgc aaggaatgtc
     6661 ccttattaat aattatttat aaaaaattgt gccggtgtac agcggcagaa tggtggttaa
     6721 tgtgaaaaat atatttgaag tggctgtttg tcagatgctg gttactgaga gcaaagagag
     6781 aaatattgac aatgctgttg aaatgctgga aattgcagca gagaacggtg cacagttggc
     6841 acttttaccg gagatgttta tttgccctta taacagcagc ttattttctc tttacgcgga
     6901 aaacatggaa agcagtaaaa ctctttctgc aatatcaaat atagcaaaaa aacacaatat
     6961 gtatatagct gcaggttcaa taccggaaaa atatgaagac catctattta acacctgctt
     7021 tgtttttgat gctaaaggta acattatcgg gagacatagg aaaactcatt tgtttgatat
     7081 tgacatacct ggcaagataa catttaaaga atcggattca ttatctgcag gtgataatat
     7141 aacagttgtt gatacggaat attgtaaaat tgctgttgcc atatgctatg atataagatt
     7201 ccctgagttt ataagaatgg ctgctttgaa aggtgccaaa ctgttagtcg tacctgctgc
     7261 tttcaacatg actacaggac ctgcacactg ggaattgctt atgcgcacaa gggcccttga
     7321 taaccaatta tatattgctg cagcatcacc cgcaagagat gaaactgcat catatattgc
     7381 ttatggaaac tctatgattg ttgatccgtg ggggatggtt ctatcacggg caggtacagg
     7441 taaagaaatc atctatatgg atatagacct tgatgaagtt gagaaaatta gaatggaact
     7501 tcctttgcta aaacatagac gcacagatat ttatagtttt aatttataga taaatatttt
     7561 aaacaatttc tttaaaacta ctccttgact tagtctggaa tgattgttaa aatatatatg
     7621 tttattaact attagctctg ttgacggaaa actccgaccg tttacatggc ttatggggat
     7681 taattaaagg aggttagtta tgcaaaagga agcaaaacaa aatattatca gcatgtacaa
     7741 gcttcacgaa aacgatacag gttctcctga agtgcagatt gcattactca cagagagaat
     7801 caatcacttg aatgaacacc ttaaaattca taaaaaggat catcattcaa gacgcggtct
     7861 tttaaaaatg gtaggacaaa gaagaggtct tttgaattac ctggaaaaga tcgatgttga
     7921 gcgttatcgt gcaattattg aaaaattaaa tctcaggaaa taatttccta aggaattata
     7981 tcttaagaga taaagcctta gactaaataa tgagcggggt tccgctcatt atttttataa
     8041 aggataaaaa gtataaattg caccttaatg tatatgtaaa ctttccgcta aatcaattta
     8101 aggaagactg ctttaacttt ttgaacttgt gaaaatattc aagcttaaaa ataagttaag
     8161 tttggatttt caataataca atttataaaa tggagaataa taatattgca gattaagtac
     8221 aaataattaa gtggcagaaa cagggaacag agaccagaga tcagagatca gatatcagag
     8281 aacagtggtc aggaatcagt ttttttagtg aaagctctac tgacaactaa tagataatag
     8341 acaatagcta gtaactgata gctgatatct aataactgaa agccgatagc cgatagctga
     8401 tagatgataa tttgtatatg atatatagta ttttatattt ggctgtttgg cttgttgcat
     8461 ctgatttctg gtgtccgttt tctgttatct gccataatat tctaaaacat cctgatctgc
     8521 agtaatttgc aaaaggaggc aatgttaaat aatgcacaaa atatttaaaa tggaacttgc
     8581 aggaagagag cttgtaattg aaacaggcaa atatgctcaa cttgcaaatg gagctgttct
     8641 ggtaaggtat ggggatactg ttgtaatttc taccgcaaca gcatcaacag aacccaggga
     8701 aggcattgat ttcttcccat taagtgttga ctatgatgaa agactttatg cggtaggaaa
     8761 aatccccggg ggttttataa aaagagaagg aaagccgtcg gaaaaggcaa tattaacatc
     8821 aagagtaata gatcgtccaa ttaggccctt gtttccaaaa gatttaagaa atgatgtatc
     8881 tgtaattaat accgtactat cagtggatca ggataattct cctgagatag catctatgat
     8941 aggtacatcg gctgctcttt caatttccga cataccgttt aaaggtccta taggcggagt
     9001 ggttttagga ttggttgatg ataaggttgt gattaacccc aatgcaaagg aaagagaaaa
     9061 aagccaaatg tatgtaaccc ttgccggcac taaagataaa ataactatga ttgaagcggg
     9121 ggctaatgaa attgctgatg atgtaatgtt aaacgctata aaaacaggac atgaggaaat
     9181 aaaaaagatt gttgaattta tagatgaaat agttgcgcaa gaagggaaac ccaaatttga
     9241 atacaagtct tttgaagtac ctgatgaaat atttaacgct atcaaagatt atgcttacaa
     9301 taggatgaaa gaagccctac tttccgcaga taagcagata agggaaggaa atgtagccga
     9361 acttacaaaa gaaataattg aacattttgc taatgtttat ccggatatgg aaactgttat
     9421 tgctgaagca gtttataaaa tggagaaaaa agctgtaaga gaactgattt tgaaagaggg
     9481 gaaaagagtt gacggaagac acctcgacca gataagacct ttgtatgcag aagttggcat
     9541 acttcctaga acccacggtt caggtctttt tcagagagga cagacccagg tgcttacaac
     9601 tgttacactg ggagctttag gagaagttca gatacttgat ggtgttgacc ttgaagaaga
     9661 aaaaaggtat atgcaccact acaatttccc gtcatatagt gttggtgagg ccaagccttc
     9721 aagaggtccc ggcaggaggg aaataggtca cggtgctttg gctgaaagag cactggagtc
     9781 tgtaatacct aacgaacagg aattccctta tgctattaga ttggtatcag aagtactaat
     9841 gtccaatgga tccacatcac agggcagcgt atgcggaagc acccttgcat taatggatgc
     9901 aggagtaccg ataaaaagac ctgtcgcagg catatcctca ggacttgtta ttgatgagga
     9961 gaatcctgac aattttatag tgtttatgga tattcaagga atagaggatt tctttggaga
    10021 tatggacttt aaagtagcag ggacaaagga aggtattact gcaattcaaa tggacataaa
    10081 agttgaaggt cttacagaag aaattatcag acaagctttc gagatgacca aaaaaggcag
    10141 gtaccagata ttagatgatg ttattctgaa aacaatacct gaaccgagaa aagaactttc
    10201 aaaatatgcg cccaaaatac ttaccacgaa tattgaccct gataaaataa gagatgttat
    10261 tgggcctggt ggcaaggtga ttaataaaat tatagctgac acaggagtta aaatcgatat
    10321 agaggacgat ggcagagttt ttgtttcaac ttcagacacc gaatcaggaa tggcagcaat
    10381 aaaaatcatt gaggggattg gcaaggaagt agaacccggc caggtatttc tgggtaaagt
    10441 agcaagaata atgccttttg gagcttttgt agaaatagca ccggggaaag agggcctggt
    10501 tcatatatcc aaacttgata agaagcgagt taacaaggtt gaggatattg taaatatagg
    10561 tgacgaaata cttgttaaag ttattgaaat agacaagcag ggtagaataa atctatccag
    10621 gaaagatgct ctaatggatg aagaaaaaca aacaaataat aatgatgtcg aataatgctg
    10681 attggggaca tacctcaata aagaagtgag aagtgagagg tgtgtcccct tacattatgg
    10741 aggaaatatg gtaaaaaagt atgtgctgga taacggaatt agaattgtat gcgaacaaat
    10801 accttatttg cgttctgtat ccataggaat atgggttgga gcaggttcca gaaatgagag
    10861 taaattaaac aatggtgttt cacattttat tgaacatatg ctttttaaag gtacagaaaa
    10921 cagaacagcg aaggacatag ctgtttctat agacagcata ggcggtcagc taaatgcctt
    10981 tacaggtaag gaatatacat gttattatgc taaaacactt gacacccata tcgatattgc
    11041 tgtagatgtc ctctcagata tgttttttaa ttctcgcttt gataataagg atattagtgt
    11101 agaaaaacag gtaattattg aagaaataaa catgtatgag gatacgccag aggaactggt
    11161 acatgatgtg ttagcagaaa taatgtggga tggagatccc ctagggtatt ctattttagg
    11221 tacatatgaa tctttggatg ccatagaccg ggaaaaaata aagagttatt taactggcaa
    11281 ttatcatccg aataatacag taattgcaat agcaggcaac tatgatgaag ataaaatgct
    11341 tgacttaata actaaatatt ttaacagatg gaaaaacgaa gagtataata cgactgacta
    11401 tagccctgtt acatataaat ccggagtaaa attaagaaat aaggatacag aacaaatcca
    11461 tttatgtgtt ggatttaatg gcattgaaaa tggaagtgat gatatatatc ctctcttggt
    11521 tgtaaataat gtattgggtg gtggaatgag ttctaggtta ttccagaaaa taagggagga
    11581 agaaggactg gtatattcta tttattccta tccatcttca tttaaaaact caggtgcagt
    11641 tacaatatat gcaggaatga accctggaca gcttgaacag gtgtcaagat taattatgct
    11701 tgaaataaaa cagcttaaaa ataggggaat atccgaaagc gagctggaga agtcaaaaga
    11761 acagcttaaa ggcaactata tgttaggtct tgaaagcacg aacagcagga tgacaagtat
    11821 aggaaaatct gagcttgtcc ttggttatgt aaatacacct gatgaaatac tggaaaaaat
    11881 agagcgagtg aacaatgata tggtaaacaa tatcataaat aaagtatttg atgtagaaaa
    11941 agcttgtgtt gcagcggtag gaaaaataaa agaaaatatt gatatagctt cattaattaa
    12001 tatagatact aaaatataga tactaaaata tagatatatt aaagatatat tgttaataat
    12061 gtgcatatgg accttatagg gtcctttttt ttatgcaatc attttcgtac agacattttt
    12121 atactgactt tattatgcct ttacttatac aagcactaat attttattat tgtcataaga
    12181 tgtattagtt atgtaagcct gggggaaatg tatggagaaa aaaagctttg ctataatcgg
    12241 gggagatcta cgaagcctta aacttatcga tttaatggaa caagatggta acgtggtaaa
    12301 agtgcatggc ttcgataatt ataaaaacat tgctaatatg gaaataaata atgatctaca
    12361 taagactata gaaggaacag atgtagttat tgggccttta ccgtgctcaa acgataatga
    12421 aacgattaat gcaccatttt gttcaaacaa aatattaata aatgatattt ttaaaataat
    12481 ggggaagaat caattgtttg ttgcaggaag aattagcagc aaaattgcac atcttgcgga
    12541 agtttataat gtatacacag ttgatattct tgacagagag gaaatggcag tcttaaatgc
    12601 tattccaact gctgaaggag ctattcagat tgcaatggaa gaaatgcaaa ttacattaca
    12661 tgacagcaat gttttagtaa tgggttttgg aagagtaggt gaagttcttg ctaaaatgct
    12721 taatggtata ggggccaata ctttcgtaga agcaagaaaa tatgctgatt ttgcatggat
    12781 aaaaagttat ggctacaagc ctgtagcaat taatcagctt gatgaatata tatctgatat
    12841 ggacgtcatt ttcaatacaa ttcctgcact tatattagat gaaaaacttt tgggcaaagt
    12901 acgaaaggat tgcttgataa ttgatctggc atcaaaacct ggcggagtcg attttgataa
    12961 ggcgaagatg atggacttga aagttaattg ggcattgtca ttacctggaa aggttgctcc
    13021 aataactgcg gcaaaattta ttaaagatac tatttataat attattgagg aattgggggt
    13081 gtaagatatg atgctaaagg gattaaaaat agggctggct ataactggtt ccttctgcaa
    13141 agttgacgat gttttccctg aagtggagaa tatggttaag gaaggagctg tagtatttcc
    13201 aataatttca gattcgattg ataaatacga tacaagattc ggtagagccg aagatacaaa
    13261 aataaaatta gaaggattga ccggcaataa aataatatca acaatagtca ctgctgagcc
    13321 cataggacca aagtctatat tagatattct tgtaattgct ccatgcacgg gaaatactct
    13381 gggcaaacta gcaaatggta ttacagatac tgcggtgaca atggcggcaa aagcgcattt
    13441 gagaaatcag aagcctattg ttattgccat atctactaat gacggacttg gagcaaatgc
    13501 aaaaaacctt ggtttattaa ttaatacaaa gaatatgtat tttgttccat ttgggcagga
    13561 tgatcctgtg aaaaaatgca attcactagt agcaaaaatg gatatgataa tacctactgt
    13621 ggttgaagcg ttggacggaa aacagataca gcctgtattg gtgtaagaag ctgatggcac
    13681 atgtaaaatc agaaacatgc aaaatgcttg agtactaaat ccatgaaata aatttttgta
    13741 gaataaattc ttcgaggcaa gtcccgaaga atttattata ttttcacctg tctgcaaata
    13801 tgttaagata gtatctagag gaaataatat ccggagggaa taatatctag atgtgataat
    13861 atctgaaaaa gattgcatct aaagcagata atatctgaag caaataatat ctaaagtaga
    13921 taatatctgg aggggatgat ctgtatgact caatttgacc ttataggtag tgttgtaaag
    13981 cttagaaaat ttgctcctgt agtcgatctt caatgggctg aatcactcag agacgaaggt
    14041 agcctgaacc tgatcgacag ctacattgtc actgatgaac tggcaggcca ttttgaaaaa
    14101 gtatttgaaa gttttacact aaaaaggcat gaaaggaaag ccctaagaga aggcagtatt
    14161 cttgatgcat cagtacacca aagagcacat atactgagag gccagtatgg taccggtaaa
    14221 tcttattttt tgcttatggt aagtgctctt ctggaatctc tggggaatga tgatcttttc
    14281 aatgatctat ataataaatt ccatgtattt gatggaatac ggttccatat ggaaaattta
    14341 aagcagagaa atgcaaaata tatggttgtc cgaattgatg gcgttaagaa tgcggacatg
    14401 cactttaacg aacttgtgca gaaaaaggtt gtttctagaa tgcaagagat tcttggagaa
    14461 acggattttt acgatagcta tagtattgct gtgcgtaagc tggaggaata caggagggac
    14521 cctatatttt caaaattgct ggaagatgca cttgtaagca gatccctgtc atacaattcc
    14581 cttattgaga gtctgaaggc ttcaaaaagg cagagcctta agaagtatcg tgagattatt
    14641 gagtccatta ccaggcacaa gataaatgag ggatttgatt ctctggaaga attcttacaa
    14701 tcagcatctt cttatgtaaa aaacaagggt tatgatggtg tggccatttt atttgatgaa
    14761 ttttccgcct atataaatgc atctattgag gatggaagga taactacaga cctcgctgcg
    14821 gtgcagtctc tggcgcagct tacaatccca agggaaggac tggatttatt ctttgtatgt
    14881 tctatgcata tagatatagg tatggtattg ggtaatgtaa tggctacagc agaagaaatt
    14941 caaaaggtca ggggcagatt tgcagaaatg accttgagct ttaacaacag tgctaacctg
    15001 gtagaaaata ttcttatagt agatagaaat aaattttcca atcttcaggc aaaataccgg
    15061 gaatactttg gagcgcttcc cagccgatat ccggatatgg cgaaagtcta tccaattcac
    15121 cctcatacgg taaacagcat tattagtgtt tccagtaaat ttgcccagaa tgaaagaaca
    15181 atcttttcat tctttgcaga gacagtgaac agaaagctca gagagccggt aattaaagat
    15241 gaacggctta acttgatcac aacaggagac atttataact attttataga tactatttcg
    15301 gaacgcaatg caattcttaa agatagtgca cttcgctgca tgtccttttg cagaacagaa
    15361 ttagaaaagg atgtaataaa agctcttgtg atagcccata tatcagcagg cgaaggttca
    15421 gactcaaggc tttcttctaa agatattgcc tttatattag gtgtagatga cataaaacag
    15481 attgatattt ttcttaaaga aatgagtgcc aatcccgctt ccaatatagt cttttatgaa
    15541 aaggaatacc gttttgaatt tattgcaaca ggcagtacag tggcagactt tacagctgct
    15601 cttgataaag atgcattgga aatagaacct tatggtgccc ttttagagct aatagaagaa
    15661 tatggaccaa gcatttgtat aaggaaaata tataatgtta atccatcaag agatttatta
    15721 ccagttagga aagacctgag gggcgttata tatagacctg cagatctttt aaaggctatc
    15781 gagcaggaaa tagataatat tgataaggat ggtagaatta tatttgttat accgggtttt
    15841 aatgataaaa tggaaaagga ttttgaagaa gctgtgaaag ccatgctttt caaagctcct
    15901 gccaatattt gtgttgctgt acctaaaagt tttccgtatg gcttagaaaa agacatcaga
    15961 ttttttactg ctgcaaggaa tatggagaag tcaggcagtg ttgatgaaaa cggcagaaaa
    16021 actttgagga aaatactcca acccatagag aaaatagtgg attatgaaat taagaagtta
    16081 ggaaatatat ttaattttac ttttattttt agcgacaaca ttgtaaaaga aaagtttaca
    16141 gctcttgaag acttttataa atttttgttg aaaaggcatt acagcaaatt cccaagagtt
    16201 gatgctgaag ctataagagg aaagaattcc attcatatga tggttgaaaa cttttttatg
    16261 tttggcgaaa aaacaaacat acctgcaaac tattcctcag agctggacaa acttataatg
    16321 gaggtcttgc agcctcttga catggttaag gttgaacgga gcggttcagg atattctgca
    16381 aggcttaaga taccggaaga agagaataac agggaatcct tcgaaatttg gaatattgtg
    16441 aacgatactt caagaccagt aagagaaatc ttcagctttc ttgagaaagc tccttacgga
    16501 ctgcctgatt atatgaccga gctttatgtt gctgcagcgg tggcagctaa tcaactggcg
    16561 ataacttaca ggggacaggt tatgcagctt aataaaatga gtatagcatt gttaaattca
    16621 gcaggatatg ctcttgaaaa agtaagaacc gcaccactgg aattgaagac agaggtgaaa
    16681 aaagtctgga gcgttttttg caaaatcaac ggacgatgcg ttgcaagaaa ttttgaaccc
    16741 gaggtgcctc aaagtgatga agatgtgcat tcgttaatag cttctgatat gtctgataca
    16801 aagattatac tggaaggtct ggagggaagg ctggaaaatg ctggtgctaa aaacagtaca
    16861 ctttttgagt tgatgaaagc gctttatgat cttcggactt tgaaaaatcc ggttaactat
    16921 atggagatat ttattcaaat gccacggaaa gtatttggta taaataacga ctttacagca
    16981 tttgagtgtt ttgatagttt ttttgtattt ctggctgaac tgagtacctc cctcgattct
    17041 ataaggcgag tagatgcaaa actaagcagt atgaggaatc tggagagcat tcaggaagat
    17101 ggtgaaacat cagaaaccac tttgtatgaa agtacttatg gagccaataa cattgcggga
    17161 ttgagaacag cttattttga aatcatcgga gattttcagc agcttaaaaa agatatctcg
    17221 aataatgttt tcctgccaga ggtcctcaaa gaactggaag gtaagcttta caagcttata
    17281 gaagattata ataaagtgtt tgtcgagctg catttagaag tcgcagataa agcaggaaaa
    17341 atccacacgc tgctcgacag ttccgcagca aaactaatag aagcttttga aagcattagc
    17401 tttagaaata tcaaaagagt ctctgacctt cgaaatgaat tgagggggat aaaagcatgc
    17461 agccataaac tgactgagaa agagcatcaa ccagaccaaa taggtctagt agatcgaaaa
    17521 aaccaggtag atcaaattaa ccggataaat agaatggatc gaatggatga aatggaccgg
    17581 atggatcgaa tatatcaaat agataatgaa ccactaaact gttcttgtac tggttttgtc
    17641 tcaggattgg cgggcctgct ggaacagata caactagtgc agcgtatgga acaatcaatc
    17701 tggaaacaga taagcaatat tggagaaaac catatattac gaattcttca acttgatgag
    17761 aaggttgaag aacgcaatat gaccttgagt gattacctca gaaaagttgg agatacaggt
    17821 gcagatatta tgtatacaag tgcagctggt acaggtaaag ccaatacagc cagtatgtca
    17881 ggcacactag gtatacccag tatctttgct gctgatataa gtgcggatgc agcctgcaaa
    17941 gatatgtaca aagacatatc cgaagataca tcaaaaaatg aacccgataa ttacatgctc
    18001 ctgcaagact gggaaaaact caaaaaatat ctgaaattgg gctttgaaac aatttcgggt
    18061 agtagtaata gtgaagaagt gattaatctg ttaggctgct tatcaaaatg cattaatggg
    18121 tatctgatag acagcattgc agtaaagaaa cagccggaag ttgaagtaaa ggtaaagaag
    18181 aaaatcgggt tccgtgcatt atatacacaa atacaatcag aaatagcaaa ttcagggtac
    18241 aagtcagtta ctgttgacga atttgcacgc acattgcagc gtataataga caaagtcagg
    18301 aaagaatatg atgaagtgaa catagaggaa tagagctgca cattgtataa gtttacataa
    18361 gattgtgcaa ggatgtatag aaagttgtaa aatatttttt gaaaacttgg agaataactg
    18421 tggaaaatga aaacatacta tcattattta aaaaggatta tgaaaccctg atggatagtt
    18481 cgaaatatat agcaaaatcc gattataatg atattaaagc cagatataac gaatatgcgg
    18541 attctataga tatatattcc cggcttgaat tcgatataga cttgcataat gagagatttg
    18601 ttaaagcgaa acttgccgaa tataaagaat actttgataa tctattatca tgtattgact
    18661 cttctatcgt tcttgatgaa gaacagcgta gggccatact tgtggatgag gattactgcc
    18721 ttgtaatagc aggtgcaggt gctgggaaaa ctactacaat ggctgcaaag gttaaatacc
    18781 tggttgagaa gttaaagata aatccagaag atattattct tatttctttt accaataaag
    18841 cagttgatga gctaaaagaa aggataatcg acaaactggg cataccagcc agagtaacaa
    18901 cttttcatgc tttcggatat gacatcctgc gaaaaataaa agaacaacct cccggtgtta
    18961 atattaatgc atataacatt atatttaact tatttgaaaa taccatttac aagaataaca
    19021 gtcttttgaa aaaactgata cttttttttg gttattattt tgatatccac gaggatgctt
    19081 tatctttcga aagtcttgag caatttcatg catataaggt tgaacaggaa tatgaaactc
    19141 taagaagcag ccttggtgaa tataatgtta atgtgattga ccggagaagt aagcaagtca
    19201 ggactattcg gggagagtac cttcgttctg ttcaggaagt tcaaattgcc aattttctat
    19261 atctgaatag tatagactat gaatacgaaa aaccatacct gtatccagtg aaaacctcaa
    19321 gaaagcctta cactcctgac ttctgggtta gtcagggaga caatacctgc tatatcgaac
    19381 attttggtat tacggaagat gggaaaagca gtatatataa tccaaaacaa ctgtccaggt
    19441 atattgaagg aatagaattt aagaaacagc ttcatgcaaa aaatcaaacc ccgcttatta
    19501 caacatattc cgcttacaga gatggaaagc agctattaaa gcatctggag gaggaactta
    19561 taaaagaggg gttcgtttta aaacccagag atcccggaga ggtataccag aaacttgcaa
    19621 attccggtaa ggataagtat attgtcaggt tcatacttct aatgattgaa tttataaggg
    19681 cattcaaaac cactggatat gaccggggca catttttatg gcttaaaaaa agaactgata
    19741 atgtcaggac acaattgttc ctaagtatag ctgaagagat atatgatatg tatcaggaag
    19801 aacttgagaa aaataatagt attgactttg aagatatgat taacgaagcc gaaaagacac
    19861 ttcaggaaat gcaggagaca ggaacaaata ttagctgtaa atacatcata atagatgagt
    19921 atcaggatat tgcaaggcag cgtttcaacc tgaccaaacg gcttgcagat attacaggag
    19981 caaaggtaat tgcagtaggt gatgactggc aatctatatt tgcattttca ggctcagata
    20041 taacattgtt tactaagttc ctaaagctaa tgggctatgg gagagagctt caaataaccc
    20101 atacatatcg caattcacag gaacttatag atattgcagg aggctttgtt caaaaaaatc
    20161 cgtcccagat aagaaaaaga ttaatatcat caaagcatat tgaaaagcct gtaatattaa
    20221 aaacatatga tgatactaaa gaactgagaa ccaacaggat taatgcagtc gttgaggcaa
    20281 taggtgaaat tatggcccag tctaatgtga ctcagcctaa aataactctg tcgaatatga
    20341 atcagccgaa tttaaatcaa gaaaaggcag ctcagacaaa tgtgactcag cggaatatga
    20401 ctcaagcaaa ggtaacccag acaaatgtgg ctcagcggaa tatgactcgg ttgaatgaaa
    20461 ctctaacgaa cattacaaaa tcaaatataa cccagtcaga tgggaaaatc tctatccttc
    20521 ttattggaag atataatttt gatgaatact atcttttaaa atcagaaagt tttatacaac
    20581 tgcccaatgg gaagcttaag tgtaataaat accctcaggc ggatatcaca tacatgactg
    20641 ctcatagttc caaagggctg gggtatgatt atgtcatact gattaatgct ttagaaggaa
    20701 aatatggttt tccttcgcag atagaggatg accctgtcat gaagcttgta acggtagaag
    20761 atagaagtat gagctttgcc gaagagagac gcctgtttta tgttgcactg acaagaacaa
    20821 agaatcgtat ttatatagta actcccgaaa gcaagccatc aaggtttgta caagaactaa
    20881 taagagattg gggtgtggaa cgtcctccta aactaaaaat gaatgcatac aataatgaaa
    20941 atataaataa tattacagac agtaaaagca acagatgccc tatatgcgaa tttccactaa
    21001 cacacaaata taacaaaaac ataggactgg aattatggat atgtactaat gagcctgaaa
    21061 tatgtgattt tatgaccaat gacaagagca ctattggtga tatatttaaa tgtccggctt
    21121 gcatggatgg ttatatgata gtaaagaaaa acaaggataa tgataataag tttttcggat
    21181 gtacaaatca tagaccagat ggtgcaggat gtaatcatat agaatatatt tccgaaataa
    21241 gtaatataaa agttaaaaag ttatttgtgt gaaacaggct aaaggaagca aagacaggat
    21301 tacccttcgg aagtggtgct acaattgtat acaattatgt ggatttacag ataaggaatg
    21361 atactccaca tgaatatcaa cttctggtta aagtagggga taatgatttg gaagatgaat
    21421 ggagacgtaa acatccctgt ccacttgaat tccaaatcta cgaaagtgaa cacataataa
    21481 aacacgaatg gtgaggcggt tacacacgac ataatataat taggcgaaag gtcttggata
    21541 gcagtgcaaa attgatttca gatgagttcg ttgcagagaa tcatgccatt atgatgtatg
    21601 atatataaat tcataatgcc aaaaagggaa tttaacaatt ggaatgaaaa ttgtattgga
    21661 ggttgtttat gaatttggat aaaaaaacca tcaataaaag cataacaatt ttggctatat
    21721 ttatctttac atgtatactg ttttcttttt gattataatt cggataattt ttagaaaatt
    21781 acaattttcc aagaaaggaa gaaataacat gaggattatt gttttacatg gccaagcgca
    21841 taaaggcagt acatataata ttacaagact ctttttggac aacctttctg atgagagtac
    21901 agaaataacc gagttttata tgccaaagga tgcgccatcc ttctgcatag gttgttttaa
    21961 ttgctttaca aagggagagg aacaatgtcc gcattctgat attgttcaac caattgcaaa
    22021 ggctattgaa gaagctgatt tgttgatttt agaatctcct tgctatgttt atggaatgac
    22081 tggtcagctc aaaacttttc ttgatcatct tgcctaccga tggatgccac atcgccccca
    22141 tcctaaaatg ttcagtaagg ttggattggt tatttcctct gctgctggtg caggtacaaa
    22201 aaaggtgaca aaagctttgc aagataattt attctattgg ggagtgccta aaatctatag
    22261 ttatggtaaa aatgttgggg cttcaagttg ggaaactgta aagctggaaa agaaactaaa
    22321 gatagaaaag gaagttatcc aaatagctgc ccaaatttta aaatgtgttg gcaaaacaaa
    22381 accgggaata aaaacaaaag taatgtttca aattatgcga atgagtcaaa aagcaaacaa
    22441 ttggaatcct actgataaag aatactggat aaaaaatgga tggctcgata aaagcaaagc
    22501 ttggtgaaag gatttgtcgc ttactataca cctgtacaat atgtatcgat aagaaatgga
    22561 gaaaacatta tatgactgaa agtaaaaaaa agaatattaa ttagaactat atgtgtaata
    22621 acatcaataa caatgatagc tctaataata actatgactt gactagtaat ataataatta
    22681 aaaaagatat ggaacgattt aaaaagcagc ttgacttttt aattgaaatt gacaaactga
    22741 agcaggtgtt tagaaatact attctcatgg atgcttcaag acttgaaaat gatgctgaac
    22801 atatgtggca tatggcagtc ggtgctatgc tcttttcaga gtattcaaat aataaagata
    22861 ttcatatgtt gaaagtttta aaatggtatt aatacatgat gttatagaaa tttatgcagg
    22921 agacactttt gcttattatg aacagaggca tgttgataaa gaagaacggg aacaaaatgc
    22981 agcaaacaaa atctttgctc tgctacctag agaacaggtt aggtggtttg agaaagattt
    23041 catggataat ctaaaagaag agctgccagt accaaagaaa ccatttatat atccatgttg
    23101 actttataac gcctgcacac caaagtacag gcgctattat tttgtctacg aactctcaaa
    23161 gatcactaaa aatattgact gatgagtagt ttcacaagct attccatatc aactattttt
    23221 ttgaattgtt cc
//
LOCUS       DAOSDD010000043        23131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_979_length_23131_cov_5.116701, whole genome shotgun sequence.
ACCESSION   DAOSDD010000043 DAOSDD010000000
VERSION     DAOSDD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23131
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_23131_cov_5.116701"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1446
                     /locus_tag="PK733_05800"
     CDS             319..1446
                     /locus_tag="PK733_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOJ10089.1"
                     /translation="MLNNSRKSNRFYNHTWFIWFALIFITPVGVFLMWKNQKYSRMFR
                     ISITTIFIIWFSVFVAAAYSNSDEYSGKDESNNRNTINSVIVDGESKDETGQSSNDKS
                     LKTDDLSQNNQAAHQPDLPIDQKTDHTEINEKREVNGKDEVNESILLDKARVKRVVDG
                     DTIEVDFGDGKTEKVRLIGVNCPESTIRQDPYGKEASEYTKKMLTDKTVYLEKDVSDR
                     DKYGRLLRIVWITEPEEISETEIRDKMYNAELVLGGYAQVSTYPPDVKYADYFKKFTS
                     EAREANKGLWALKESKKSDTDTDIYTDTDTDKDKTAEPEQNNKVQTSSLDIRKIKGNI
                     NSKGEKIYHVPGGMYYDKTIPEEWFETEEEAKAAGYRKSMR"
     gene            1647..2090
                     /locus_tag="PK733_05805"
     CDS             1647..2090
                     /locus_tag="PK733_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ10090.1"
                     /translation="MDDIDIHILKILSNHARLTVSEISNQVNMSIPAVSERLKKLESS
                     GIIEKYTIILNSRKLEKDLTAIMLVSIERTKSVGDFLNIVENSKDVLECHYIAGDYDY
                     LLKIVTENTLTLEALLNKIKSVRGVQKTKTVIVLSTIKNNPSILP"
     gene            2497..3669
                     /locus_tag="PK733_05810"
     CDS             2497..3669
                     /locus_tag="PK733_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOJ10091.1"
                     /translation="MKLNGLTIGVPKEIMKGERRVAATPDTVAKMVKEGAKVLVEAGA
                     GAGAYFLDEDYSGHGAQIVNDVCDLYSKADIILKVKEPQYNHEKDRHEVNMMRKGQCI
                     VTFLHPASPGNHDMVRQLAKNDIVSLTLDGVPRISRAQAMDALTSMSTVAGYKSVLLA
                     ANRLPKFIPMIGTAIGMIQPSNVMVIGTGVAGLQAIATAKRLGAVVHAVDIRPDACEQ
                     AKSVGAKVVETGVPAEVAIGKGGYAQHLSEDWLKKEREVLKGIVPKMDIIILSALVPG
                     KIAPVLITEEMVKSMKIGSVIMDIAIDQGGNCAVTQAGEVTEKYGVTLDGTKNIPGML
                     PTSSTWMFANNIYNFLCNLVQDGKITINTEDQIIASSLVTYKGEIVHAGAREAMRI"
     gene            3746..4039
                     /locus_tag="PK733_05815"
     CDS             3746..4039
                     /locus_tag="PK733_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOJ10092.1"
                     /translation="MQAFLQILVFVITTFIGYKVIKNVPSLLHTPLMSGMNALSGITV
                     LGALVSTAAAVGTGSKILGVVAIIFAMINVVGGFCVTDRMLKMFKTQSEVKGK"
     gene            4036..5571
                     /locus_tag="PK733_05820"
     CDS             4036..5571
                     /locus_tag="PK733_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOJ10093.1"
                     /translation="MSVTLYYILGLILVFGIIWGIRLMNSPKTAARGNLLGAVSLMGT
                     VLLTLYKNEILGSSLLWVSMILGSIIGYYIAVKVTMLKMPQMIALLNGLGGGASGLVA
                     IMILTGSQGDVDLFNRGAAVLAMLVGWITFSGSMIAAAKLAQKLSQRPTHLKGHSVIL
                     NVTSVFICMLIVLLLMGSTAGSFGSSLFLSLLMLVASLFWGVIFTIRVGGADMPITIS
                     LLNSLSGLAGAIAGFAIYDPILVAVGGIVGAAGLILTQVMCKAMNRSLMNVLTGKTAV
                     ASNKHTTSGHTVNSGKADVLVKANISVKADASAGQGKQEHSGSGKQQVVETVKEAATV
                     IEAPVKLVNKEDESVRLKRIVEEAGSVIIVPGYGMALAQAQYQVKSLYEMLMEKGKDV
                     KFAIHPVAGRMPGHMNVLLAEVDIPYDKLYELDDINEEFSNTDLVIVIGANDVINPAA
                     NTAEDTPIYGMPILQVDQAKNIIIFNWDTKPGYSGVPNTIYELDKVILRLGDAKETLK
                     AII"
     gene            complement(5711..7114)
                     /locus_tag="PK733_05825"
     CDS             complement(5711..7114)
                     /locus_tag="PK733_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10094.1"
                     /translation="MNRRRCIGAANVALIIGIAGIVLIFTSIVGVLLAGSLMEQKKSN
                     GKADEVANIIEETDSLTSNQSNEKPSGTSKGTSKGTSDSLPDSSSNNPSGNFLDSLSD
                     YYPNYPQNRQELTEKLEEYKKANKDLVVYQGPVRHIFFHPLIAFPELAFDGDYISQGF
                     DDWFITVKEFNKILESLYNKNYILIDIKYMYEESEEVGKKLIPRRDMLLPPGKKPLII
                     SIDDMNYYKYMRQNGVVHKLILDSEGNISDFSITPSGNELITKENDIVTILDRFVMEH
                     PDFSFMGAKGIAALTGYEGILGYRTDNPPAPNIEEEKKEALKIIKRLKETGWSFACHG
                     YGHLDTVKIGLDVLKKDTQRWKDEVEPLIGPTPVYIFPFGSGVLPGSEKSNYLKEAGF
                     PVMCAVGNGDYIKYESDVIYMDRIHIDGIAFRDRMPVLKEMFEIDDIVDEVRPWKNQP
                     NEAPDTQTPGADAPQDI"
     gene            7338..8162
                     /locus_tag="PK733_05830"
     CDS             7338..8162
                     /locus_tag="PK733_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOJ10095.1"
                     /translation="MQRISFKACAIVNEVDLNKIAVKCDIRKKYTWEEPLILKGNTLN
                     TILNKKVSEGQAIMLFSFGSIVFINLPSSYIHIFLNYMKEYKPEIDIKDWERYSDDYE
                     LRAGNVEDIEFTDDYAMVPQIEVAYIELVSIVLAKSVALEKIEEQLEKILDRIESMID
                     RLEKGNLRFSDKELAKATARIMRHEYNSISYIMILDKPDITWLNSVAAEFYDNMAEFF
                     ELNDRFEILKSKTDILGSIVDGFSSISHAMRGIFVEWVVVILIILEVILMIAEFFK"
     gene            8186..9082
                     /locus_tag="PK733_05835"
     CDS             8186..9082
                     /locus_tag="PK733_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOJ10096.1"
                     /translation="MREVGFDCFKIAPRINLKNIAAFFNLSWPASGRVEFIVLEDSQI
                     ETILKYGTHSKVAYIFEFGCITLVNFETDEINIFMEYIESITNKMQSGMILKYYERYE
                     AEIDNSGFFKLYDESESCIRYNRNVEVILSVIMAKSTALFRVESEVDELMDKAEKYVD
                     YLQKGWLHTHTRKFSAITARILRFEYDSIYSIEILDRIIGFDKKVKLREIYDEIAEYY
                     ELGDRFNILRNKVDNLRKITRAYTKLSYERHENRLYLFEVFLLILFPLSYLFRYLIKY
                     KRLETMLPKLFTSILNFDILRK"
     gene            9092..10306
                     /locus_tag="PK733_05840"
     CDS             9092..10306
                     /locus_tag="PK733_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ10097.1"
                     /translation="MQGIVLEGGGAKGAYHIGVCKALNDLGLEIGAVAGTSVGALNGA
                     MIVQGKLEQAFDIWYNMDPDRIIKIADSDRKELNRMEDQGERLGAKLEKLRKIITDRG
                     LDISPLEDILKEVINEAEVRNSIIDFGIVTFDLTDRKPVELYKENIPEGKLIDYLIAS
                     ASLPWFKTKTIDGNVYVDGGVYNVLPVNLVQKKGYTDIIVVRTYPPLGRVRKFDETGL
                     NIISIGPEESLGPILDFSAERARRNLSLGCFDAKKAILKLKGRKYYIEPSSNNEDFYV
                     KYFTNLDDKKIKSVCELFGIEYAYGKRALFEHIIPKIASILEVPSNASYEDIIIALIE
                     DAARILEIEQFKIYTMDDLLSLIRKKHRPAGDEIVKQIPAFIRNMNIVSKIARDKIIG
                     DIAIELLGGMGI"
     gene            10586..11797
                     /locus_tag="PK733_05845"
     CDS             10586..11797
                     /locus_tag="PK733_05845"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOJ10098.1"
                     /translation="MADRIKMKIPLVEMDGDEMTRVIWKMIKELLLEPYIELKTEYYD
                     LGLEKRDETDDSITVEAANAIKKYGVGVKCATITPNAERVKEYNLKQMWKSPNGTIRA
                     ILDGTVFRAPIVVNSIKPSVRTWTKPITIARHAYGDVYKNVEYRVEGPCKAELVFTSK
                     EGKEQRLTVHEFDGAGVVLGMHNLDSSIKSFARACFNYALNQRQDLWFSTKDTISKKY
                     DHTFKDIFEDIFENEYKHIFKEAGITYFYTLIDDAVARVIRSDGGFIWACKNYDGDVM
                     SDMVATAFGSLAMMTSVLVSPDGHYEYEAAHGTVQRHYYRHLKGEETSTNSMATIFAW
                     TGALRKRGEIDGIAELVEFAEKLEKSSIRTIEEGVMTKDLAVLSEAEDKKVVNTKDFL
                     LEIKKRLEGVI"
     gene            12025..13050
                     /locus_tag="PK733_05850"
     CDS             12025..13050
                     /locus_tag="PK733_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10099.1"
                     /translation="MVKVGLIGLGFMGRTHLDNYIRLESEGYPVKLTAICDVDSGKFE
                     GNLTGGNIGAGSNKYDFSKYKLYTCMDEMLEKEELDYVDIALPTYLHAEASIKASNKG
                     LHVLCEKPMARTSEECEQMIEAAKKNGKKLMIGQCLRFWPEYEILKDYVDSGRFGKVT
                     SAYFYRGGGTPIWSFENWLLQNDKSGGCLLDQHIHDVDTINWLFGKPEKVSTLGKNIV
                     PGSGYDIVSTNYFYADGKVVNAQDDWTLNGDYGFEMLYRVNFEKGNLVFKDGKLTVNP
                     NDGKSFVPEHPAEMGYYREIVYFIDAVLNDKPTDKAPTESTMETIRIAEAEVASADNN
                     GAITVVK"
     gene            complement(13195..14823)
                     /gene="groL"
                     /locus_tag="PK733_05855"
     CDS             complement(13195..14823)
                     /gene="groL"
                     /locus_tag="PK733_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616331.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ10100.1"
                     /translation="MAAKIILFDEDARKAMEKGVNKLADTVKITLGPKGRNVVLGKKF
                     GSPQVVNDGVTIAKEIELEDPYENMGAQLVKEVASKTNDIAGDGTTTATLLAQAIVRE
                     GLKNVASGANPMILKKGMDKTVNKVVELLKQRSEKIKGREDMVFVAAISSSDEQIGTL
                     IADAMEKVSKDGVITIEESKSSETYIEVVEGMQFDRGYISPYMVTDTEKMQAVLENPF
                     ILITDKKISNIQDLLPALEFVVNNSGKLLIIAEDIEGEALATLVVNKLRGTLQCVAVK
                     APGFGDRRKEMLQDIAILTGGQVISDDTGMDLKDIELEWFGRSKSVKVEKENTLIVDG
                     AGDSSNIKKRVESIKKQIELTTSDYDKEKLQERLAKLSGGVAIIRVGAATETEMKDKK
                     LRVEDALNATRAAIEEGIVAGGGTAYINTIPEISKFIEDLEGDQKTGGMIIMKALEEP
                     LKQIAINAGTDGSVVVDKVKNSPKGIGFDALKEEYVNMIQAGIVDPTKVTRTAVQNAA
                     SIATMILTTESTVAEKPKKEKTPPAAPGGYDMDY"
     gene            complement(14931..15494)
                     /locus_tag="PK733_05860"
     CDS             complement(14931..15494)
                     /locus_tag="PK733_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOJ10101.1"
                     /translation="MIVAVYGSPRTDGNTDILMDTFLDAVQTQEKVVKFKLRDMLLKP
                     CTGCCKCYKTGICVFNDDIWSIYEHIEKADGLILSSPIYFASVTAQLKTFIDRGQAFW
                     VRKFILKQQQKAPGKKGFYISAGAMTVDKYFINSRLVIRSFMVSLDIKCAGELFFPGI
                     DKKGEIRNQPEALNSAYKAGLDFTTIY"
     gene            15765..17552
                     /locus_tag="PK733_05865"
     CDS             15765..17552
                     /locus_tag="PK733_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10102.1"
                     /translation="MRAKQILLDFVKEHKWRYLVGFIFLLVTSIITSVMPKIIGLITD
                     SLKDRAPASVVYKYLFLLVLGAAGIFIFRFIWRYFLVGNSRYLECHLRAKLFEHLQTL
                     PVNFYNNSKTGDLVAYAINDIQAIRRTFAFGLTAILDGVVVNTISIVLMAKTINPILT
                     IMALAPAPVIVFVITKLRRKIRERFTEVQKAFAQISEKVQENISGIRVIKAFAQEKEE
                     TADFDSYSMKRVNTHMKLIRISAALGPVTQICFGISFVFFIIYGSELVGKGIISLGDY
                     VAFNSYISVIMGPILNIGRLVEVWQQGIASSKRLDTIFDVKSEFESLEKQDDKAIDEK
                     REIKGSIEFINLTFKYPGTAKRVLKDINLKLDEGQTLGILGKTGCGKTTLANLLLRLY
                     DVKPGHIFIDGRDINSIPANILRENIGYVPQDNFLFSTSIKENIEFFRHIYRDWEVME
                     VAKMAGVYDDIISFPDGFDTIIGERGVTLSGGQKQRVSIARALIKNPPVLILDDSLSA
                     VDTKTEEEILGNIKKVLANKTGIIISHRVSTIKHADQIIFMDRGRIIERGTHEQLIDM
                     KGKYYELYVSQTETQTRELSEEGCNNGSR"
     gene            17539..19356
                     /locus_tag="PK733_05870"
     CDS             17539..19356
                     /locus_tag="PK733_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10103.1"
                     /translation="MEVDNVPKTKTISEQKSGKKNKTQFIRLLKYFKPYIPLVLLSIL
                     FAFLINGAVIAKPYIIKYIIDEYLAKGNYDFKIFRLAGILYFLVVLIGAAFDYVEDYM
                     LTYVGQKIMSDIRKQLFEHIQTMSMKFFDRNSTGRILTRVTNDVESLNELFSGMIVNV
                     IRDFIMLASIVVAMILMNAKLALLSMCSIPLIIIITVIYRKAARKNFLKMKSMIGKIN
                     GFLAENISGMKLVKIFHREKEKYEELENFDKEYFASSLREVILNSFSRPIVEVINTLT
                     IALIVWYSIGRIGSGALEIGVLYAFITYSKQFFRPINDIADKYTSIQSALISSERIFE
                     IMDNHEYIEDLKTGVPIDKLEGRIEFKNVWFAYNEEEWVLKDVSFTVESGQTIAFVGA
                     TGSGKSTIINLIARFYDIQKGQILIDGVDIKEYNLRDLRRHISVVMQDVFLFSGSIKS
                     NIRLKNTSITDEDIKHAAELVCADKFIESLPGKYDEEVKERGATFSAGQRQLISFARA
                     TAFKPSIFVLDEATASIDTETELAIQNAMSNISGGRTTIIIAHRLSTIRNADNIIVIH
                     KGRIREMGTHDELLDKRGIYRQLYEKQLNTEAEKCVTVF"
     gene            19531..21945
                     /locus_tag="PK733_05875"
     CDS             19531..21945
                     /locus_tag="PK733_05875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ10104.1"
                     /translation="MKVAKNLNVLKVYLMVLLIILIFACCSIRGDNCKFLTFNDNCAI
                     PEIEKGFLDLRGWNFDKSGVIRLNGEWEFYWEQIINPGEFDESKHKEFISLPRSWNGF
                     NINEKVLQGDGYATFRLILMLDSNLEFAGLEIPPIKSSYNIWINGKLYNKVGVVGSNA
                     EEFYPKYYSDIVYFTPDKANTEIVIQAANFMHAKGGIWQPVVLGTAYQIKALREKQLA
                     IDMFLFSSLLIIAVYNILIYLTSKKDKYALYLGLFSLLISGRSILTGGIDFIQTIRDI
                     NQERILKLEYLTLGLGVTFFTLYIDKIFQVKKINSEDNRVSNKKSSKINFNSKIYNVM
                     KYFLYYSGILFSVFVILMPARIFSRFLIAFQIIIVVAAVYFISFFTYTLIEKKEKAYL
                     ILAGSIFFFGTIINDILYYNSLVFTRGITPLGLLVFIAVCSLLQPVNFSWYIKNLREM
                     TGHAISVYGRGTEKDNNLLKKTTVDLSEELASTLELNKVLERLLVKLKGVLGFDAGIV
                     MLKEGDMLKVYYKLGCMNENKAGCLNENIKNGITKELTDHKLIYTVVESRKPLIIENE
                     NDHFSIFGEENYFIKSVLALPIEKSSEALGIIILMSTEENYFNEHNAEIAFNFTSRSA
                     MAIENAKLFSEVKRLASEDDLTQIYNRRYFFELAEREFKHHKRYKDLKPLSVIMLDID
                     DFKCINDTYGHYAGDVVLKKVAKVCRDVLRETDLVGRYGGEEFVMLLPHTERTEACIV
                     GERLRKNLANCPFQYNDELINVTVSMGLAVMDENMRDLDELMIKADKALYMAKDKGKN
                     CIVTLP"
     gene            complement(22074..23033)
                     /locus_tag="PK733_05880"
     CDS             complement(22074..23033)
                     /locus_tag="PK733_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOJ10105.1"
                     /translation="MATIIIILVNIVAGSACLIYGVNLISDGLEKINLHRIRHVLTRF
                     TKNVPMAFLTGTIITALVQSSTAVTVITVGLVNTGIIGFSQAIGIIYGANVGTTITAQ
                     FMSFKITDFAPAFISLGLAIKLLGKKYSVRNVGRATIGFGLMLLGINILGWSMPYMEK
                     SEFVRDIFLKYGKNPYLGIIVGMLATMIVHSSSATVGFTIVLFNSGFISFDAALGLVL
                     GDNIGTCFTTVLASIDTTISAKRTAWAHVLYNIIGSLTALALLSPFSELVKKFTVMIG
                     QDMSRLVANAHTLFNILSALLFLPFTRYYERFVVWIIGNEGIR"
BASE COUNT         7803 a         3602 c         4636 g         7090 t
ORIGIN      
        1 tattatatac catacttcaa caaattacat cttatttttt ataatatatt taaaaaaatt
       61 tgtcaatgtt tttttcaaat ttaggtgggc gcatttttta attgcacttt tacatgttga
      121 aaaggcttac acaaggtatt gagtatttcc agagaaatac acaaaagagt acaaaaaaat
      181 aacaaattgc agaatattac ataattcgac aatatagtat aaaatccctt attttttgca
      241 ttgaataaac tacctgtcag ctttacaatg aactatgaat acattagtaa tgagagttat
      301 tgtgaggtga cagtaaatat gctaaataat agtagaaaat ccaatcggtt ttacaatcat
      361 acatggttta tttggtttgc acttatattt attactcctg taggtgtttt tctcatgtgg
      421 aaaaaccaga aatatagcag gatgttcaga atatcaatta ccactatttt tattatatgg
      481 ttttcagtgt ttgtagcagc agcttacagc aattctgatg aatattcagg aaaagatgaa
      541 tcaaataata ggaacacaat caactctgta attgttgatg gggaaagtaa agatgaaacc
      601 gggcaaagct caaatgataa atcattaaaa actgatgatt tatcccaaaa taatcaggct
      661 gcccatcaac cagaccttcc gattgatcaa aagactgacc atactgaaat caatgagaaa
      721 cgtgaagtca atggaaaaga tgaagtcaat gaatcaatat tactggacaa agctagagtt
      781 aagagagttg tggacggaga tactattgaa gttgactttg gggatggaaa aactgaaaaa
      841 gtaaggctta ttggagttaa ctgtcctgag agtacaataa gacaggatcc ttatggcaag
      901 gaagcttccg aatacacgaa aaaaatgctt accgataaaa cagtttatct tgagaaggat
      961 gtaagcgaca gagataaata tggcagattg ctaagaattg tatggattac agagcctgaa
     1021 gagattagtg aaactgagat aagagacaag atgtataatg cagagcttgt tttaggtgga
     1081 tatgcacagg tttcaacata tccgcctgat gttaaatatg ctgattattt taaaaagttt
     1141 acatcagaag ccagggaggc aaacaaaggc ttgtgggctt taaaagagag caaaaagtca
     1201 gatacagata cagatatata tacagataca gacacagata aagataaaac tgcagaacca
     1261 gaacagaata ataaagtcca aacttcgtca ttagacatta gaaaaataaa aggtaatata
     1321 aattctaaag gtgagaaaat ttaccatgtt ccgggaggga tgtattatga taaaacaata
     1381 ccggaagaat ggtttgagac tgaagaagaa gctaaggctg caggttatag aaagtctatg
     1441 aggtaaaaat atagggttaa attcatatcg agcatgaaac aatatggaag aaaatgcacc
     1501 ccccactagt agtaagtgct tgagatgtgt gcttgttaat gttaaaatat atatgaagta
     1561 tttgtcctaa gcgtaaacat attgcggaag ccggttaaag ggagcagtta gcttaaagga
     1621 gtagttgcga agggggacgt gttaatatgg atgatattga tattcatatt cttaaaatct
     1681 taagtaatca tgcaagattg acagtatctg aaataagcaa tcaggtcaat atgtctattc
     1741 ccgctgtaag tgaaagactc aaaaaattgg agtcatccgg cattatagaa aagtatacta
     1801 taatcttaaa ttcaaggaag cttgaaaaag acttaactgc tattatgttg gttagtattg
     1861 aaagaaccaa atctgtcggt gattttttaa atatagttga gaattcaaaa gatgtcctgg
     1921 aatgccatta catagctggt gattatgatt accttcttaa aattgtaaca gaaaatacct
     1981 taaccctcga agcgttgctg aataaaatca agagtgtccg cggtgttcaa aaaactaaaa
     2041 cagtgatagt tttatcaact ataaagaaca atccttcaat attgccctaa gaatattaac
     2101 tattaatcag acgcatagat gtaaaatatt gtgtgattat ataagccaat aatgtactac
     2161 cttaaatatt taagcaaata tgaaaaagat agttattata gaataaataa ttaggtataa
     2221 gtaataagtt attaatatgt tttcgatttt tacatgaaat atttacatta tttcggaccc
     2281 aaattataat acaagtatag tataattata gttgtacata aatttagtaa atagtgaatg
     2341 gtatatggta aattattatt cattataatt tcaaatgtaa caaattctaa actagtttta
     2401 atgcagcaaa acataaaaaa attaataaca aaatcaaaat cgcagtcaac caataaatgg
     2461 aatttatgac tgctcagtta aaagggggca ttttacatga agttaaatgg gcttacaatt
     2521 ggagtaccaa aagaaatcat gaaaggtgaa agacgtgtag ctgctactcc tgacacagta
     2581 gcaaagatgg taaaagaagg ggcaaaagta ttggtggaag caggggcggg tgccggtgca
     2641 tacttcctcg atgaggatta tagcggtcat ggcgcacaaa ttgttaatga tgtttgcgat
     2701 ttgtattcaa aagcagacat tatattaaag gtaaaagaac cacagtataa ccatgaaaaa
     2761 gaccgtcatg aagttaatat gatgcgtaaa ggccagtgta ttgtaacatt tcttcacccg
     2821 gcatctcctg gaaatcatga tatggtcaga cagcttgcaa aaaatgatat tgtatctctt
     2881 actcttgatg gagtgccgag aatttcaaga gcacaggcta tggatgctct tacttcaatg
     2941 agtactgtag cgggatataa aagtgttctg cttgcagcta acagactgcc taaattcata
     3001 cctatgatag gaacagctat cggtatgatt cagccttcaa acgtcatggt cattggtaca
     3061 ggtgttgctg gtctgcaggc tattgccact gctaaaagac ttggagctgt tgtacatgca
     3121 gtagatataa gaccagatgc ctgtgaacaa gctaaaagtg tcggtgcaaa ggttgtagaa
     3181 accggtgtac ctgccgaagt tgccatagga aaaggcggat atgcccagca cctctcagag
     3241 gattggctga aaaaggaacg cgaggtcctt aagggtatag ttcccaaaat ggatattata
     3301 atactttctg ccttagttcc tgggaagatt gctccggtat taattactga agaaatggta
     3361 aaaagcatga aaattggctc tgtaattatg gatattgcca tcgaccaggg aggtaactgt
     3421 gctgttaccc aagcaggaga ggttacagaa aaatacgggg ttactcttga tggaactaag
     3481 aatatcccgg gaatgcttcc cacaagttct acctggatgt ttgcaaataa tatatataat
     3541 ttcttgtgta atcttgtaca ggatggaaaa attacaatca acacggaaga tcaaataatt
     3601 gcgtcatcgc tggttacata taaaggcgaa atcgttcatg caggtgcaag agaagcgatg
     3661 aggatataat atagaagtaa tattgtacta aagtactaaa gtacaaaagt atgaaagtat
     3721 aaaatttaac agggagagat gatttgtgca agcatttttg caaattctag tttttgttat
     3781 aactacattt attggttata aagttattaa aaatgttccc agcctgttgc atactccttt
     3841 gatgtcaggg atgaatgctc tgtccggaat aacagttttg ggggcacttg tgtctacagc
     3901 tgcagcagtt ggaacaggta gtaaaatcct gggtgttgtt gctataatat ttgcaatgat
     3961 aaatgttgta ggtggttttt gtgttacgga ccgaatgctg aaaatgttta aaacacagtc
     4021 ggaggttaaa ggaaaatgag tgtaacattg tattatatac taggtttgat ccttgttttc
     4081 ggtataatat ggggaatcag attaatgaat tctcctaaaa cggcagcacg aggaaacctg
     4141 cttggggcag tatccctcat gggtacagtt ttattgacat tatataaaaa tgaaatactc
     4201 ggcagttctc ttctatgggt tagcatgatt ttgggaagta ttatagggta ttatattgca
     4261 gtaaaggtta caatgctaaa aatgccgcag atgattgctt tattaaatgg tctcggcgga
     4321 ggagcttccg gtttggtggc tattatgata ttaacaggaa gtcagggtga tgttgattta
     4381 ttcaacagag gtgcagctgt tctagcaatg ctggtcggat ggattacttt cagcggcagt
     4441 atgattgcag cagcaaaact tgctcaaaag ctttcccaac gtccaacaca cttaaaagga
     4501 cattctgtta ttttaaacgt aacttctgtt tttatatgta tgctaatagt actcttgtta
     4561 atgggatcca cggcaggttc gttcgggagt tcactgtttt tatcattatt aatgttagtt
     4621 gcttcgcttt tctggggtgt tatttttaca atccgtgtag gtggggcaga tatgccaatt
     4681 accatatctc ttctgaattc attgtccggt ttggcaggtg ctattgcagg ctttgctatt
     4741 tatgatccaa tattggtggc tgttggtggt attgtaggag ctgcagggct tattttaacc
     4801 caggttatgt gcaaggcaat gaaccggagt cttatgaatg tacttacagg taaaactgct
     4861 gttgcttcta ataagcatac aacatcaggc catacagtta attccggtaa agctgatgtc
     4921 ttagtcaaag ctaatatctc agtcaaagct gatgcttcag ccggacaggg caaacaggaa
     4981 cattcaggtt caggaaagca gcaggttgtt gaaacagtta aagaagcagc aactgtaata
     5041 gaagctccgg ttaaactggt taataaggaa gatgaaagtg taagactaaa aaggattgtt
     5101 gaagaagcag gcagtgtcat aattgttcct ggttacggca tggctctggc acaggctcag
     5161 taccaggtca agagtctcta tgaaatgctt atggagaaag gcaaggatgt gaaatttgcc
     5221 attcaccctg tagcagggag gatgccgggg catatgaatg tattgctggc ggaagttgat
     5281 attccttatg acaagctata tgaactggat gatattaacg aagaattcag caataccgac
     5341 cttgttattg taataggtgc taatgacgtt ataaatcctg ctgccaacac agctgaagat
     5401 actcctatct atggcatgcc tattttacag gtagaccagg ccaaaaacat aataatcttc
     5461 aactgggaca caaaaccggg atattcaggt gtacccaata caatttatga gcttgataag
     5521 gttatcttaa ggctggggga tgcaaaggaa acccttaagg caattattta agatagatgg
     5581 agtgcatttg taaaatctga agaaatattt accaggggct attgttacgg tgcttagagc
     5641 cttcactaat gcccatagcc aatatttctt cagataaatt aaatacaccc agctgaaatt
     5701 aagttatatc ctaaatatct tggggtgcat cagcccctgg agtttgggta tcaggtgcct
     5761 cattaggctg gtttttccat ggacgtactt catctactat atcgtcaatt tcaaacatct
     5821 ccttaagtac aggcatacga tctctgaaag ctattccatc aatatgaata cgatccatat
     5881 aaataacatc tgattcatat ttaatataat caccgtttcc tacagcacac attaccggaa
     5941 atccggcttc tttaagataa ttggattttt cacttccggg cagaacaccg ctgccaaaag
     6001 ggaaaatata aaccggggta gggcctataa ggggctctac ttcatccttc catctttgag
     6061 tatctttctt aagtacgtca aggcctattt ttacagtgtc aagatgacca tagccatgac
     6121 aggcgaaaga ccagcctgtt tctttcaggc gcttgattat tttcagagct tctttctttt
     6181 cttcttcaat atttggcgcc ggaggattgt cggttctgta gcctaatata ccttcatatc
     6241 ctgttaaagc cgcaatgcct tttgcaccca taaaagagaa atccggatgc tccatcacaa
     6301 acctgtcaag tattgtaaca atgtcatttt cttttgtgat aagttcattg ccggaaggag
     6361 taatggaaaa atctgatata ttaccttcag aatccaatat cagtttgtgt acaaccccat
     6421 tctgtctcat gtatttatag taattcatat catcaattga aataattaat ggctttttgc
     6481 cgggcggaag cagcatatct cttcttggaa tcagtttttt gccgacttct tccgattctt
     6541 catacatata ttttatatca ataagaatat agtttttatt gtaaagagat tccagtattt
     6601 tattaaattc ctttaccgtt ataaaccagt catcaaatcc ctgtgatata taatctccgt
     6661 caaaagccag ttcagggaag gcaattaggg ggtgaaaaaa tatatgcctt acaggaccct
     6721 gataaacaac cagatctttg tttgccttct tatattcctc aagtttttca gttagttctt
     6781 ggcgattttg ggggtaatta gggtaataat ccgaaagact atctaaaaaa ttgccggaag
     6841 gattatttga agaactatct ggtagactat ctgaagtacc ctttgatgta ccttttgatg
     6901 tacccgatgg tttttcattg ctttgattgc ttgttaatga atcggtttct tcaataatat
     6961 ttgcaacttc atctgctttc ccatttgatt ttttttgttc cataagcgag cctgccaaca
     7021 aaactcccac aattgaggta aagataagta caataccggc gataccgata atgagagcaa
     7081 catttgcagc cccaatacaa cgtcttctat tcatttcaaa aaagttcctt tcattaattt
     7141 taatattgcc attatactta atgctgatga ttaatgctaa tgtttttgta catattaatt
     7201 acatattact attaatagga tattttttca gttaaaaatt agttaaaata ttctaaaatt
     7261 ctggtataat atataatagt tgatataaaa aagcaattat gctggtgcat aagggcggaa
     7321 tggcggttat tataataatg caaagaatat ctttcaaggc ttgtgctata gttaatgagg
     7381 ttgatttaaa taagattgcg gtaaaatgcg atataaggaa gaaatatacc tgggaagagc
     7441 cccttatatt aaaaggaaat acactaaata caatactgaa taaaaaagta tctgaaggac
     7501 aggcaataat gttattctct tttggcagca ttgtgtttat aaacctgcca tcaagttata
     7561 tacacatttt cttaaattat atgaaggaat acaaaccgga gattgacata aaggattggg
     7621 aaagatattc tgatgattat gaattgcggg caggtaatgt tgaagatatt gaatttacag
     7681 atgattatgc aatggtaccg caaattgaag ttgcatatat agagttggta tctatagtgc
     7741 ttgcaaaatc cgtagcactt gaaaagatag aagaacagct ggaaaaaatc cttgaccgta
     7801 tagaaagcat gatagacaga ttggagaaag ggaatctcag gtttagcgat aaagaacttg
     7861 ctaaagccac cgcaaggatt atgagacatg agtacaacag tataagttat attatgattc
     7921 tggataagcc ggatatcaca tggctgaata gtgttgcagc agaattttat gataatatgg
     7981 ctgagttctt tgaactgaat gacaggtttg aaattctaaa aagcaagaca gatatattgg
     8041 gaagcatagt agatggattt tcatcaataa gccatgcaat gcgaggcata tttgttgaat
     8101 gggtagttgt tatactaata atacttgaag tcatactcat gattgcagag tttttcaaat
     8161 agaaaaaagc caggaggtag agagaatgag agaagtcggg tttgattgtt ttaaaattgc
     8221 tcctagaatt aatcttaaaa atatagctgc ctttttcaat ttatcctggc ctgcatcagg
     8281 cagagtagaa tttatagttc tggaagacag tcagattgaa acaatattga aatatggtac
     8341 ccattcgaaa gtcgcttata tttttgaatt tggatgtatt acacttgtga atttcgaaac
     8401 tgatgaaata aatatattca tggagtatat tgaatcaatt accaacaaaa tgcaaagtgg
     8461 tatgatatta aaatattacg aaagatatga ggccgaaatt gataatagcg gttttttcaa
     8521 attatatgat gaaagcgaga gttgcataag atacaaccga aatgtggaag ttatactttc
     8581 agtcattatg gcaaaatcca ctgcgctttt cagggttgaa tcagaagttg atgagcttat
     8641 ggataaggct gaaaaatatg tagattacct gcaaaaaggc tggctgcaca ctcatactag
     8701 aaaattttct gctataactg caagaatatt aaggtttgag tatgatagca tatatagcat
     8761 tgagatactt gatagaataa ttggttttga taaaaaagtg aagctgaggg aaatatatga
     8821 tgaaattgca gaatactatg aactgggaga caggtttaat atactaagaa ataaggtgga
     8881 taacctaaga aaaattacca gagcttacac aaaattaagt tatgagcggc atgaaaacag
     8941 gctgtatcta tttgaagtat ttctgcttat actttttcct ttatcatatt tgtttagata
     9001 tttgataaaa tacaaaaggc tggaaactat gcttccaaaa ctttttacca gcattttaaa
     9061 ctttgatatt ttaagaaagt aggggaagat tatgcaagga attgtattag aaggcggcgg
     9121 agcaaaaggc gcatatcata taggcgtgtg caaagcttta aatgatttgg gtttggaaat
     9181 aggagcagtt gcaggtactt cggtaggtgc actaaatggc gcaatgatag tccagggaaa
     9241 attggagcag gcttttgaca tatggtataa tatggatcca gatagaataa ttaagatagc
     9301 ggacagtgat cgcaaagaat taaaccgtat ggaagatcag ggggaaagat tgggagccaa
     9361 gcttgaaaaa ctgaggaaaa tcataactga caggggactt gatataagcc ctcttgaaga
     9421 tattcttaaa gaagttataa atgaggctga agttagaaat tccattattg attttggtat
     9481 agttaccttt gacctgaccg accgaaaacc tgtggaatta tataaggaaa atatacctga
     9541 aggtaaatta atagattacc ttatagcaag cgcaagcctt ccttggttta agaccaagac
     9601 catagatgga aatgtttatg ttgatggggg agtatataat gtacttccag ttaatttggt
     9661 gcagaaaaaa ggatatactg atatcatagt ggtaaggaca taccctcctt tggggcgtgt
     9721 aagaaaattt gatgaaaccg gcttaaatat aataagtatt ggtcctgaag aaagccttgg
     9781 accaatattg gatttcagtg ctgaaagggc tagaagaaat ttatctcttg gctgttttga
     9841 tgctaaaaag gcgattttga aactgaaagg caggaagtat tatatcgaac cttcatcaaa
     9901 taatgaggat ttctatgtta agtattttac aaaccttgat gacaaaaaaa tcaaatcggt
     9961 ttgcgaatta tttggcattg aatatgccta tggaaaaaga gctctatttg aacacataat
    10021 acctaaaatt gcaagcatac ttgaagtacc atcaaatgcc tcatatgaag acataattat
    10081 tgcactgatt gaagatgcgg caagaattct tgaaatagaa caatttaaaa tttatactat
    10141 ggatgatttg ctttcactaa tcaggaaaaa gcacaggcct gcaggagatg agatagttaa
    10201 gcaaatacct gcatttataa ggaatatgaa tattgtttca aaaatagcga gagacaaaat
    10261 tattggggat atagcaatag agcttttggg aggtatgggc atttagaatt aggtgcggcc
    10321 atggcaatta aagacttgta aatttacctg atttagttta tattttaatt aaagaattat
    10381 caatgctatg ccaatttgga aagcgatgtc tttcgtccaa gaaatgcgct ttccattggc
    10441 aacagaacaa agtagtatta taatgtattt atagctaaag cttatagttg agataaatac
    10501 acttatttga gttcacacgc atgcatagct cgtacacgca tataaagcaa ataaattaat
    10561 taaatacata gtaagggaga ttgatatggc agacagaatc aagatgaaga tacctctggt
    10621 tgagatggat ggggatgaaa tgacaagggt aatctggaag atgataaagg aattgcttct
    10681 ggaaccttat attgaactta aaactgaata ttatgacttg ggtttggaaa aaagagatga
    10741 gacagatgac agtataactg tagaagcagc aaatgcaata aaaaaatatg gtgttggtgt
    10801 aaaatgtgca accataactc caaatgcaga gagggttaaa gaatataatt taaaacagat
    10861 gtggaagagt ccaaatggaa ctattagagc aattcttgat ggtactgtgt tccgggcgcc
    10921 tattgtggta aacagtataa aaccttcagt aagaacctgg acaaaaccta taaccattgc
    10981 cagacatgct tatggtgatg tttataagaa tgttgaatac cgtgttgaag ggccatgtaa
    11041 agcagagctt gtatttactt caaaggaagg aaaagaacaa agactgactg tacacgaatt
    11101 tgatggagca ggtgtagttc ttgggatgca caaccttgat agttcaatta aaagctttgc
    11161 cagagcctgt tttaattatg cgctaaatca aaggcaggat ctgtggttct cgactaaaga
    11221 tacgatttct aaaaaatatg accatacatt taaagatatt ttcgaggaca tattcgaaaa
    11281 cgaatataaa catatattta aagaagccgg tataacatac ttctatactc tgattgatga
    11341 tgcagttgcc cgcgttataa gatcagatgg aggttttata tgggcttgca agaattatga
    11401 tggggatgtt atgtccgata tggttgcaac agcatttgga agtcttgcta tgatgacatc
    11461 ggttttggtt tcacctgatg gccattatga atacgaagct gcccacggaa cagttcaaag
    11521 gcattattac cgtcatctca aaggcgaaga aacatcaaca aactcaatgg caacgatttt
    11581 tgcatggacc ggtgcattaa ggaagagagg agaaattgac ggaatagcag agcttgttga
    11641 atttgctgag aaactggaaa aaagctctat aaggacaatt gaagaagggg ttatgacaaa
    11701 agatctggcg gtactttcgg aagcagaaga taagaaggta gtaaatacta aggattttct
    11761 gctggaaata aagaaacgtc ttgagggagt tatttgatgt cttggcactt aattgcagag
    11821 acatttatag ataacgcaca aaagaaacta cattataaaa gttttgtggc ccatattaga
    11881 ttatttttag ggttagtgga ccacaacatt aattaatacg caaaaattca atttgttcct
    11941 tgtaaaatca tttttgcgtt atatttagta taataataac taataaaaac accacttggt
    12001 ggtaaatatt aaggaggctg gaaaatggtt aaggtaggtt taataggact gggtttcatg
    12061 ggaagaacgc atcttgataa ttacataagg cttgaatcgg aaggttatcc cgtgaagttg
    12121 actgccatat gcgatgtgga ttccggaaaa tttgaaggta acctgactgg aggcaacatc
    12181 ggggcaggta gcaataagta tgatttcagc aagtataagc tttatacttg tatggatgaa
    12241 atgcttgaaa aagaagagct ggactatgta gatattgctc tgcccacata ccttcatgct
    12301 gaagcttcaa taaaagcttc aaataaagga ttgcatgttt tatgtgagaa gcccatggca
    12361 agaacttcag aagaatgtga acaaatgatt gaagctgcta aaaagaatgg gaaaaaattg
    12421 atgattgggc aatgcctgcg tttctggcct gaatatgaga tccttaaaga ttatgtagac
    12481 agcggcaggt ttggcaaagt aactagtgca tatttctaca gagggggcgg aactccaata
    12541 tggtctttcg agaactggct tctgcaaaac gataaaagcg gaggatgctt attagatcag
    12601 catattcatg atgttgatac tataaactgg ctttttggaa aacctgaaaa agtttctaca
    12661 cttggaaaga acatagtgcc tggaagcggt tatgatattg tatcaaccaa ctacttctat
    12721 gcagatggaa aagttgtaaa tgcacaagac gattggacct taaatggaga ttatggtttt
    12781 gaaatgcttt acagagtgaa ttttgaaaaa ggcaatctgg tctttaaaga tggcaagctt
    12841 actgtaaacc caaatgatgg taaatcattc gtaccagaac atcctgctga aatgggatat
    12901 tacagggaaa ttgtgtattt tatagatgct gttttgaatg ataaacctac agacaaggca
    12961 cccacagaga gtacgatgga aacaatcagg attgctgaag ctgaagttgc ttctgcagat
    13021 aataacgggg ctataacagt tgtgaagtaa attgatttat gcgggtgtat ttatcatata
    13081 tgcagaaata tttatcaata tttttcttat ataaattaaa tgcacctctt aagtaatctc
    13141 aaaataccat aaaaaaggct taccggacta aataatccga caagcctttt ttcattaata
    13201 atccatatca tatccgcctg gagctgcagg cggagtcttt tccttcttag gcttttctgc
    13261 taccgtactt tctgtagtaa gtatcattgt agcaatagat gcggcattct ggactgcggt
    13321 tcttgtaact tttgtagggt caacaatacc ggcctgaatc atgtttacat attcttcctt
    13381 caaagcatca aaaccaatac ctttaggact gttcttaacc ttatcaacaa caactgaacc
    13441 gtcagtaccg gcgttgattg caatctgttt caggggttct tcaagtgctt tcatgataat
    13501 cattcctccg gtcttttggt ctccttccag atcttctata aacttgctga tttcaggaat
    13561 agtattgatg taagcggttc cgccaccggc cacaattcct tcttcaatgg cggctctggt
    13621 agcatttagt gcgtcttcga ctcttagttt tttgtctttc atttcggttt cagttgcagc
    13681 gccaaccctt attattgcta ctccaccgga gagttttgca agtctttcct gtaatttttc
    13741 cttatcataa tctgatgtgg ttaattcaat ctgcttcttg atagattcca ctctcttctt
    13801 tatatttgaa gaatcaccag caccgtcaac aattaaggtg ttttcctttt ctaccttaac
    13861 agatttggat ctgccaaacc attcaagttc tatatcttta aggtccatac ctgtatcatc
    13921 ggaaataacc tgaccgcctg taagaattgc tatatcctga agcatttcct ttcttctgtc
    13981 accaaagcct ggagccttaa cagctacaca ttgcagtgtc cctcttaact tgtttactac
    14041 aagtgtcgca agagcttcac cctcaatatc ctcggcgata attagaagtt ttccgctgtt
    14101 atttacaaca aattcaaggg caggcaataa gtcttgaata ttgcttatct tcttatcggt
    14161 tataagtata aaaggatttt cgagaacagc ctgcattttt tccgtatcag ttaccatata
    14221 tggtgaaata taacctctgt caaactgcat gccttctacg acttcaatat atgtttcaga
    14281 agattttgat tcttcaatag taataacacc gtcttttgaa actttttcca ttgcatccgc
    14341 aatgagcgtg cctatttgtt catcactgga tgaaatagca gctacaaata ccatatcttc
    14401 tctgccctta atcttttcac tacgttgttt tagtaattca acaaccttat taacagtttt
    14461 atccattcct ttttttaata tcataggatt tgcaccagaa gccacgttct taaggccttc
    14521 tcttactatg gcctgagcaa gaagggtagc agtagttgta ccgtcaccgg caatatcatt
    14581 tgttttactt gcaacttcct ttacaagctg agcacccata ttttcgtaag ggtcttccag
    14641 ctcaatttct tttgcaatag ttacaccatc attaacaacc tgcggtgaac caaatttttt
    14701 gcccaagaca acatttcttc cttttggccc aagtgttatt ttaactgtat cagccagctt
    14761 gtttactcct ttttccatag cctttctggc atcctcatca aaaagaatta tttttgcagc
    14821 cataaacaat ccctccatca ttacttcaat tttgtttatt agcactctat tgcaacgagt
    14881 gctaatagtt ataattttat ttattttgta ttgttctgtc aagaggtatt ttaataaatc
    14941 gtggtaaaat ccaggcctgc tttgtaagct gagtttaaag cttccggctg attccgaatt
    15001 tccccttttt tatcaattcc ggggaaaaat aattctcctg cacactttat atccagtgaa
    15061 accataaaac tacgtatcac aagccgggaa ttaataaaat atttgtcaac ggtcatagca
    15121 ccggcagaaa tatagaaccc ctttttccca ggtgctttct gttgttgctt tagaatgaat
    15181 ttcctgaccc agaaagcttg tcctctatca ataaaggttt tcaactgtgc agttacagat
    15241 gcaaaatata taggagagga taatatgaga ccatctgctt tttcaatatg ctcatatata
    15301 ctccaaatat catcattaaa tacacatatc ccggttttat agcatttaca gcatccagta
    15361 caaggtttta aaagcatgtc ccttaattta aatttaacca ctttttcttg tgtctgtact
    15421 gcatcaagaa aagtatccat taaaatatcg gtatttccgt cagttcgtgg acttccgtat
    15481 acagcaacaa tcattcgtaa atcctccgtc tctatagtgt atgcaggtat tgtattgtta
    15541 gtgtttttaa cccttttttc acatataaat aggatatcac aaaattctaa tacttaaaac
    15601 agggactatt gcaggggtaa ggtaaaaaat gatgacatta gtaaataatt aggttataat
    15661 ataaagtaaa taaaaactgg tggttatatc ttcaggtatt tattgaaacg gtgttaagca
    15721 atccgattta ttttaaatat tagcttatta ttaaggggtt aaaaatgaga gcaaaacaaa
    15781 tacttttgga ttttgttaaa gaacataagt ggagatattt agtaggattt atttttctcc
    15841 ttgttacgtc cataattaca tctgtaatgc cgaaaataat tggtttaata actgatagcc
    15901 tgaaagatag agcacctgct tcagtcgttt ataagtatct atttcttctg gtgctgggag
    15961 ctgcaggcat ttttattttc agatttatct ggaggtattt tttagtagga aattcacgtt
    16021 atctggaatg tcatttaaga gcaaagcttt tcgaacatct ccaaactctt ccggtaaatt
    16081 tctacaataa tagtaaaaca ggtgatcttg tagcttatgc aataaatgat atacaagcaa
    16141 tcagacgtac atttgcattc ggattgacgg ctatacttga tggtgttgta gtaaatacta
    16201 tatcaattgt tttaatggca aaaacaataa accctattct tacaattatg gcgttagctc
    16261 ctgcgccggt tattgtattt gttataacaa aactaagaag gaaaatacga gaaaggttta
    16321 ctgaagtaca aaaagcattt gctcaaattt ctgagaaggt tcaggaaaac atatcaggaa
    16381 ttagggtaat taaagccttt gcgcaggaga aggaggaaac tgcagatttt gatagctaca
    16441 gcatgaaaag ggtaaataca cacatgaaat tgataaggat ttcagcggct cttggtcctg
    16501 taactcagat ttgttttgga atcagctttg ttttctttat aatatatgga agtgagcttg
    16561 ttggtaaagg cataatttcc ttaggggatt atgttgcatt caactcgtat atttctgtaa
    16621 ttatggggcc catattgaat ataggaagat tagtagaagt atggcagcaa ggaattgcat
    16681 cttcaaagcg gcttgataca atttttgatg ttaaaagtga gtttgaaagc ttggaaaaac
    16741 aggatgataa ggctatagat gagaaacgcg aaatcaaagg cagcatagaa tttataaatc
    16801 ttacttttaa atatcccgga acagcaaaaa gagtacttaa ggatataaat ctaaaacttg
    16861 atgaaggtca gactctgggc atattgggta aaacaggctg tggtaaaacg actctggcca
    16921 atttgctgct aaggctttat gatgttaagc ccggccatat ttttattgac ggacgggata
    16981 taaatagcat acctgcaaat atattaagag agaatatcgg ttatgttccc caggacaatt
    17041 ttctgttttc tacaagtatt aaagaaaata tcgagttttt caggcatata tatagggatt
    17101 gggaagttat ggaagtggct aagatggcgg gagtatatga tgatataata tcatttcccg
    17161 atgggtttga cacaataatc ggggaaagag gggttacact ttcaggcgga caaaagcaga
    17221 gggtttcaat tgcaagggca cttataaaaa atcccccggt attaatactc gatgacagtt
    17281 tatctgctgt agatacaaag actgaagaag aaatcctcgg taatattaag aaggttcttg
    17341 caaacaaaac cggaattata atttctcaca gagtttcaac tataaagcat gctgaccaga
    17401 taatttttat ggacagggga aggattattg agagaggaac acatgaacaa ttgattgata
    17461 tgaagggtaa atattatgag ttatatgttt cgcagacaga aacccaaacc agggaattat
    17521 ctgaggaggg gtgtaataat ggaagtagat aatgtaccaa aaactaaaac catatcggag
    17581 caaaaatcag gcaaaaaaaa taaaacccag ttcataaggt tgttgaaata tttcaaacct
    17641 tatatcccat tggtcttgct cagcatatta tttgcatttt taataaatgg ggccgtaatt
    17701 gcaaagccat atattataaa atacataatt gatgaatatc ttgcaaaagg taactatgat
    17761 ttcaagatat tcaggctggc aggaatatta tattttctgg tggtgctgat aggggcagca
    17821 ttcgattatg ttgaagatta tatgctcaca tatgttggac aaaagataat gagtgatata
    17881 aggaagcagc tttttgaaca tattcaaact atgtctatga aattttttga caggaattcc
    17941 acaggaagaa ttttaacaag agtgaccaat gatgtagaat cgcttaatga acttttttca
    18001 ggtatgattg ttaatgttat aagggatttt ataatgctgg caagcatagt tgtcgcaatg
    18061 atattaatga atgccaaact tgctttatta agtatgtgca gcataccttt aattataatt
    18121 ataactgtga tttaccgcaa agcagcaaga aaaaactttt taaagatgaa atcaatgatt
    18181 ggaaagataa atggcttcct cgctgagaac atatcgggaa tgaagcttgt taaaattttc
    18241 cacagggaaa aggaaaaata tgaggaactt gaaaactttg acaaagaata ctttgcttca
    18301 agtttaaggg aagtaatatt aaatagcttc agcagaccta ttgtagaagt aattaacact
    18361 ctcactatag cgcttatagt ctggtacagt atagggagga taggctcagg tgcacttgag
    18421 ataggggtac tttatgcgtt tataacttat tcaaaacagt tcttccgacc aataaatgac
    18481 atagctgaca agtatacatc aattcaatcg gcactaatat cctccgagag gatattcgag
    18541 ataatggaca accatgaata tattgaggat ttaaagactg gggttcctat agataagctg
    18601 gagggcagaa tagaatttaa aaatgtctgg tttgcatata atgaagagga atgggtatta
    18661 aaagatgtaa gttttacagt cgaatcaggt caaactattg cttttgttgg agctacaggt
    18721 tccgggaaat ctacaataat taatcttatt gcaagatttt acgatataca gaagggccag
    18781 atattaattg atggtgtaga tattaaagaa tacaatttgc gagacttaag aaggcacatt
    18841 tccgttgtta tgcaggatgt attccttttc tcgggtagta ttaagtccaa tattcgattg
    18901 aagaacactt ctatcacgga tgaagatatt aaacatgcag cagaactggt atgtgcagat
    18961 aaatttatag aatcacttcc cgggaaatat gatgaggaag taaaggaacg aggagctacc
    19021 ttttctgccg ggcaaaggca gttaatatct tttgccaggg caaccgcatt caaaccttcc
    19081 atttttgtac ttgatgaagc tactgcaagt atagatacgg agacagagct tgctatacaa
    19141 aatgccatgt caaacatttc cggagggagg actactataa ttattgctca cagactatct
    19201 accattagaa atgcagataa tattattgtt attcataaag gcaggatcag ggaaatgggt
    19261 acacatgatg aactgcttga taagagagga atatacaggc agctctacga aaaacagcta
    19321 aatacagaag cagaaaaatg tgtaacagtt ttttaggaaa taatatgcat ttgtataaaa
    19381 atttataaaa aatagtggta aatttcttac aaatattata agataacatc atatacaatt
    19441 tgatgtatgc aaaaatgact tggtcacagg ccattaatat accgaatata ccaatttatt
    19501 aaaggtggta aaaggccggg gggaattttt gtgaaggttg cgaagaattt aaatgtatta
    19561 aaagtttatc ttatggtttt gttaataatt ttaatttttg catgttgcag cattagaggc
    19621 gataattgca aatttctgac ctttaatgat aattgtgcca tacctgaaat tgaaaaaggg
    19681 tttttggatt taagaggctg gaattttgat aaaagcggtg ttataagact taatggagaa
    19741 tgggagtttt actgggagca aattataaat cccggagaat ttgatgaaag caaacataaa
    19801 gaatttataa gccttcccag gtcatggaat ggctttaata ttaatgaaaa ggtattgcag
    19861 ggagacggat atgctacctt tcgcttaatt ttaatgctgg attccaatct ggaatttgcg
    19921 ggactggaaa tacctcctat aaaatcgtct tataatatat ggataaacgg aaagctttac
    19981 aataaagttg gggtagttgg aagcaatgca gaagaatttt atcctaaata ctattcggac
    20041 atagtatact tcacacctga caaggctaat acagaaattg tcatacaggc ggcaaacttt
    20101 atgcatgcaa agggtggtat ctggcaaccg gttgttctag gtacggctta tcagattaaa
    20161 gctttaaggg aaaagcagct tgccatagat atgtttttgt tttcaagcct gctaattatt
    20221 gcagtttaca atatactaat atatttaaca agcaaaaagg ataaatatgc actttacctt
    20281 ggtttattca gcttgcttat atcaggaaga tcaatactta caggtggaat agactttatc
    20341 cagacgataa gagacattaa tcaggagcgc atccttaaat tggaatatct tacccttggc
    20401 ctcggcgtta cattcttcac tttatatatt gacaaaatat ttcaggtaaa aaaaattaat
    20461 agtgaagata atagggtaag taataaaaaa agcagcaaaa taaattttaa tagcaaaata
    20521 tataatgtaa tgaagtactt tttatattat tcaggaatac tgttttctgt ttttgtaata
    20581 ttgatgcctg cacggatatt cagcaggttt ttgatagctt ttcaaattat aatagtagtt
    20641 gcagcagttt attttatatc cttcttcacc tatacattaa ttgaaaagaa agaaaaggct
    20701 tatctgattc ttgcaggttc aatattcttc tttggaacaa ttattaacga catactatat
    20761 tataactcat tagtattcac aagaggtatt acacctttgg gcctgctggt ctttatagct
    20821 gtgtgttctc ttttgcagcc tgtaaatttc tcctggtata ttaaaaattt acgtgagatg
    20881 accggtcatg ctattagtgt atatggaaga ggaactgaaa aagataacaa tttattaaaa
    20941 aaaactacag ttgatttatc agaagagctt gcatcaactt tggagcttaa taaagtactg
    21001 gaacgattac ttgtaaaatt aaaaggtgtg cttggctttg acgcaggtat agtaatgctt
    21061 aaagaaggag atatgttaaa agtatactat aagttgggat gtatgaatga gaacaaggct
    21121 ggatgtttga atgagaacat taaaaatgga attacaaaag aattgacaga ccataaactt
    21181 atatatactg ttgttgaaag ccgaaaacct cttataatag aaaatgaaaa cgaccatttt
    21241 tccatattcg gagaagaaaa ttatttcatt aaatcagttt tagcattacc aatagaaaaa
    21301 agcagcgaag cattaggtat tattatccta atgagtacag aggaaaatta ctttaatgaa
    21361 cataatgctg aaattgcttt taattttact tcccggtcgg caatggctat agaaaatgca
    21421 aagcttttca gtgaagtaaa gagacttgca agtgaagatg atcttaccca aatatataac
    21481 cgcagatatt ttttcgaact ggcagaaaga gaattcaaac accataaaag atataaggat
    21541 ttaaagcctc tttcagtcat aatgcttgat attgacgatt ttaaatgtat taatgacaca
    21601 tatggacact atgctggaga tgttgttctg aaaaaagtag caaaagtatg cagggatgtt
    21661 ttacgcgaaa ctgatttagt gggacgctat ggtggagagg aatttgtaat gcttctaccg
    21721 catacggaga gaacggaggc atgtattgta ggggaacggc tgcgtaaaaa tttagccaac
    21781 tgcccatttc aatataatga tgagttgata aatgttacag taagtatggg gttggcagtt
    21841 atggatgaaa atatgagaga ccttgatgaa cttatgatta aggcagataa agctttgtat
    21901 atggctaaag ataagggtaa gaattgtata gtgaccttgc cttaatgggt gcattttgtg
    21961 gttcattttc tggtgcattt tctagatata aggagatatt taccatgggc tgccgttaag
    22021 gtgcttagag cctttaaatt cagctacggg caaaattaca ctcacaaaat aacttacctt
    22081 ataccttcat ttccaataat ccatactaca aatctctcat agtacctggt aaagggtaga
    22141 aacaacagtg cactcaatat attaaataat gtatgggcat tggcaaccag tctggacata
    22201 tcctgcccta tcataacagt aaattttttt accaattctg aaaaaggact tagcaatgca
    22261 agcgcagtga gacttccaat aatattatat aaaacatgtg cccaagcggt acgtttagcg
    22321 gatattgtgg tatctatgct tgctaaaact gttgtgaagc aggttccgat attgtctccc
    22381 agaactaagc ccagggcggc atcaaagctt ataaaacctg agttaaaaag aacaatagta
    22441 aaaccaacgg tggcactgct gctgtgaaca atcatcgtag caagcatacc tacgataatt
    22501 ccaagatatg gatttttacc atacttaaga aaaatgtccc tgacaaattc acttttctcc
    22561 atgtaaggca tgctccaacc aagtatattt atccccaaaa gcattaatcc aaaacctatg
    22621 gttgctctgc caacgttcct aacagaatat tttttaccca ataacttaat agccaatcca
    22681 agagatataa aggcaggggc aaagtcagta atcttaaagg acataaattg agctgtaata
    22741 gtagtgccaa catttgcacc ataaataatt ccaatagcct ggctaaaccc tattatgcca
    22801 gtattaacaa ggcctacggt aatcacagtt actgcagtac tgctctgcac aagagcagtg
    22861 attattgttc cggttaaaaa agccataggt acattctttg tgaagcgtgt taatacgtga
    22921 cgtatacggt gcaaatttat tttttccaac ccatcgctta ttaagtttac tccgtatatt
    22981 aaacatgcac ttcctgctac tatgtttaca agtataatta ttatagtcgc caaattttac
    23041 ctccaatatg ccaaataggt tctcccagat gaggatgcca aatggggaca gtcctctata
    23101 tatgctatat atgtggggac agtcctctac t
//
LOCUS       DAOSDD010000044        22560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1006_length_22560_cov_5.179116, whole genome shotgun sequence.
ACCESSION   DAOSDD010000044 DAOSDD010000000
VERSION     DAOSDD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22560
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_22560_cov_5.179116"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..646
                     /locus_tag="PK733_05885"
     CDS             41..646
                     /locus_tag="PK733_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ10106.1"
                     /translation="MNQLRLRNAFSLAFYAPSITSGVAMSVVWLYFFSNDRYGLINNI
                     LINLGITSEPILWTMDPKYILSVVIFISIWMSMGTGFLVFLAGLQNVPKEYYEAGAID
                     GIKNKFQELWLITFPTLKPQLLFGAINSIVGSFGVFDIAVSVAGMPSPNYAAHTIVAH
                     LYDYAFIRFQMGYASTVAIVLFLMTFLMGRLVMKMLSSKDI"
     gene            671..1600
                     /locus_tag="PK733_05890"
     CDS             671..1600
                     /locus_tag="PK733_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10107.1"
                     /translation="MSNETSMEYPVKIIKRLRGSHSTRPIRSYKSNMTLPEVLLYIFM
                     ILLVGFTALPLVYMISTAFKPLDELFIFPPRYLVRKPTIQNFHDLVISLGSSTVPFAR
                     YIFNSLYVTVITVTCTVIVSSLGAYGLVKHKPRGSKAIFNFIIAALMFSPHVTQIPRY
                     MVVNSLGLIDSYWALIIPNIAVAYNFFLMKQFTEQFPNELLDSSRVDGANEWIVFWKI
                     VMPSLTPAWSTLVVFSFVSNWNDYFSPLIYITSRAKKTLPPALQTIGAGGSIARAGAV
                     AAATLLMTLPTVIIFTFMQSKVMETMTYSGIKG"
     gene            1614..3764
                     /locus_tag="PK733_05895"
     CDS             1614..3764
                     /locus_tag="PK733_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ10108.1"
                     /translation="MRAFKSKKAMSFLLVIVVVILIVNSIAFAESRDYILDGNERIPI
                     PETYILKHVINRLENAGEEVKYFNDADDLFINKEGYLFVADTGNNRIVKLTADGRLVA
                     VFKGSENKPFNSPMGVYADEFGNMYVADTGNQRVVHLSADGEFVEEFLKPESELLGAS
                     YTFSPSKLCISPTGYLYVTKGQNVMTIDAYNRFRGFMGQNEIGFKLLDAILRMFASQE
                     QKRAIAKRTAAAYLNLTMDEKGMLYAVTMDSADGEIKKLNSIGENIYRKYGGIQQDMK
                     LLSLDFITKMKLKSTSFVFGERKDEEGKSIKPFFKDIAVDKNGVVTVIEQQTAKIYQY
                     DQEGNLLTVFGGKKAQKGGFSIPTSLAVDPQGCIYVLDRGLGNIQVFEPTRFIKLVHN
                     AVKLYSDGDYDRSYAAWQEVLKTHENYRLAHEGLAKTLFKQKKWKDAMEEFKEADDRN
                     GYSKAYIEYRYYIFRKYFTLVVLCIAAIVVGLIYFIKGIRKLSESGLEQFRNGGTKLF
                     SICDVLKFSTAIIFHPVETFELIKSNRERISVKPGVIILLLAFVSRILYMFVVHYPLA
                     DIDLRDSNIILEAVKLLLPVVTWVVASFAITAITDGESKINEIFIASSFCMIPYILIN
                     TPLALLSNILSRGEQTLYGFIQNGTWIWILILFFIQVKILNDYKTPKTTATCVVSALN
                     MGLIWAVCLMIYVLSGRLVEFITGIIQEIKMIVI"
     gene            3784..6012
                     /locus_tag="PK733_05900"
     CDS             3784..6012
                     /locus_tag="PK733_05900"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ10109.1"
                     /translation="MLVKKQNGNKALVLIVSLALLLTAKFPLKSEAAPLNVQLSGEYV
                     EALKTESLLLYCNLNTGAIAVFDRRNGHMWKSTIDIESDEGAFKNVGVSWKKSMSSLF
                     AISYINMNKNDVIIEKAFSTSDADDIKVTFPNNKVMMEYSFGKQGIKVAVEIMLEGDN
                     LVVKVPVDKIEENDVYGLIALELMPFFGASDRSVDGYIFYPDGCGAIMKFENVEKRPK
                     DSKPYKAFIYSPEEVSLKEYGQMKKDQQYQASLPVYGIKNGDNAFLAVVTKGEEETAI
                     QVSPEGYAVNLNWAGVEFKYRHFYNLILSNITINGKDIAKKTTSSKPDKELVLQDHEV
                     RFMFIAGQNANYSGMANSYREYLIKNNQLKQAVKPDGNIPLALDIFMGIKEKRMLFDK
                     FITMTSFEQAKIITEKFLSNGVKELQINLIGWSKGGYGAYPVNWPPERLLGGISGLNK
                     YLGYTKENNVSVFLQAGFLHALGDNGGFSQKNDIAIQGNSLPVTNIDKNRFILNPFSA
                     FNRFTEFLKKLNTFPYAGVALEDLGEFVYHDYNKKQPSGRNDTVQKWKDILNASLIEE
                     KPLAVGGGNAYVLKYADRLFNIPVNSSNYHINDETIPFYQMVVHGMIPYTSEPGNLFF
                     DPVKQKLQWVEYGCMPYFELTYESAIQLKHTKYNRLFNSNYESWLQTITEIYEEFNQR
                     LKGIWSEKMAEHKKVNDKLVKIRYSNGTTIYINYDEKETYYDGYVIEAQDYLIVEKGS
                     GF"
     gene            6012..6920
                     /locus_tag="PK733_05905"
     CDS             6012..6920
                     /locus_tag="PK733_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ10110.1"
                     /translation="MKKFRIGAEKRNILEGYIFVLPFILGTLIFFAFPLYMSIKLSFG
                     KLQRIEGFIIRWIGFGNYLKAFITDTKFIPMFVGVFRHTLIKAPLTIIFALIIAILIN
                     KNVKFRGFFRTVFFIPFLLGMGEVMKQLLGQGVDRQVVSISGGRLLPRELLMYLGPKV
                     VEAIDSFFGIIIVVLWGSGVQILLFLSGLQSIPASLYESSKVDGATEWEMFWKITFPM
                     ISPILLLNIVYTLIASFTDITNPILEYIQNLAFKGMEFEYAATIGWIYFVFIIILVML
                     VFILLRNYVYTTDAKEAKRNDRRSSY"
     gene            6898..7917
                     /locus_tag="PK733_05910"
     CDS             6898..7917
                     /locus_tag="PK733_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10111.1"
                     /translation="MTEEAATKKWRTSNNKTQYSIPGFIRNMQERMAERRALKLSDRV
                     FRIGTYILLFDLAFVFLFPFFYMIVTSLKTNADLYNFTVNWVPRTVKIQNYVIAFRAL
                     DYIKYLKNSLILTLLGTAGHILACSFIGYGFARYDFPGKTLLFSIVILSLIVPVQTII
                     IPQYMIFCNLKWLNTYLPMIVPTFFGYGLKGGLFIFIFRQFYLGLPKALEDAAKIDGC
                     GFFRTYWNIVLPVAQSALLVTLVLSLVWHWNDFYEPSIYISKMMKTPLPSRLNELVNL
                     VNTPPSEEIFDSLIELEAMEEEMAINNAVLMAGTFLVVIPVMTAFAFLQSKFMQGIER
                     TGLVE"
     gene            8132..9769
                     /locus_tag="PK733_05915"
     CDS             8132..9769
                     /locus_tag="PK733_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10112.1"
                     /translation="MKKSFTIMLVLMILLTSFTGCVKKTDSNDANVQTSGKSSEKPVQ
                     STKEVSNFNPVGLPIVNEKITLKMMGSRHSAQGPWEDTIVMKWLEEETNIHMDVYSVP
                     SDVYAEKKNLAFASGDLPDIFFKATLSVKDEEAYGSQNLLVSLNDLIPKYAPNFSKIL
                     KEKPHVKKAISSIEGKIYSLPYVYYTNSMANVRYINTKWLENVGMKIPETIDDFYQAL
                     KAFKDMDPNGNNQKDEIPMTDVNIGGASFYLLPAFGFVTSAGNRWDAKDNKAVYIPLQ
                     NEYKEFIIYMRKLYSEGLLDQNIFTHKTAELDAKLGEYRVGVMNDSLAKIPEEKQDEY
                     RVLPPLTSHLNSQKMTYLIPGYVTGTYAITNKNKYPEASMRWVDLFYSEPKDAINGIC
                     GFTMFRGKEGEHWVYENDERTLFSHIPLPDGKSGDYYLSPFRNGGAPMYITSALSNKN
                     SLLSTRIKVEGSAEHYFPYMNRVGYPGLRYTEDENEKKNSIEDELGKYVSQMEVKFIL
                     GEEPIENWDAFIERIKSIGIDEVIKIRQTALDRWNSF"
     gene            9953..12031
                     /locus_tag="PK733_05920"
     CDS             9953..12031
                     /locus_tag="PK733_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10113.1"
                     /translation="MSVIQRVLIMVLAVLVIFGSLIGCNNSIPNSQGKQTTLDGKNRE
                     NANNKLALENGGFVMYDDTSKNIKIENEYISLEINDEGKLLAFTDKISGNSYLNKEEY
                     FVMAAKGSFNKYFPVRAAFENGNLKTISTEAVNEGKIRFELANSEGDKVGAVVGIKVY
                     NHYISFEINEITNQDSINDITFANLNTNINGTVSTKVNACYNNDFAVCVMALTLPVHC
                     APETVDSSTILNAKCYSEFGIKGNKFAIIATPFKTFLETVQEVEENENLPSPKLGGKW
                     GKLSDDVRKSYLFIRNLTEENADDVIRFVKEMDAGMILFGSVVWRATAGHYNINKRSF
                     PNGVEGMKAVVDKIHSVGLKAGLHFLPAEITVSDPYVTPVPDKRLYKDGVAVLTADIS
                     NETITIDCSEEKSANSAFPIKVIDAYRETGTDVLIDDEIIHYKIKNINRSAAGKVEIT
                     LEDCVRGAYGTKKASHKSGTKIYHLRRVYGNFQIDLETTLLDEVSTRMADIFNYCGFD
                     MIYFDGSEKLQQLPSYTSPNTNHWYYNAKLQYTFYNKLDRKENILVQGSSNSHFGWHI
                     ISRHAIADGKKDIKRSLDSNMGKIEANNRDFIPIDIGWYSIGASHISYEDIEYILCRS
                     IGFNSSVGFQESSSIGSRIANDMVELIKKYEKLRLENYFPEEVKEMLRKPGIDYKLIQ
                     DSQGRWIFVQ"
     gene            12122..13237
                     /locus_tag="PK733_05925"
     CDS             12122..13237
                     /locus_tag="PK733_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ10114.1"
                     /translation="MVNKERMVNEFIELVEIDSLSYKERDMADRLKNKLTAIGYDVYE
                     DDAGKKVGGNTGNLICKIKGNKDVPAIMLVAHMDTVVPGIGKKAVIKGDVIVSGGATV
                     LGADDISGIECILEVVRVLKEKNISHGDIYAVFTIAEEKGLIGAKNLDYEKIDAKYGF
                     VLDVGGKSGNVAIRQPIKHATTITVLGKAAHAGSRPEEGINAIQIASKAIAGMKLGRI
                     DSETTANIGIIRGGEASNIVCDRVDIKAEARSLNKEKLETQINHMKECFLSAAKEYGG
                     SMEFESKQTSPGFSMDENDEIIKILKKTAELTNFNLNLISTGGVSDTCILNSKGIQAV
                     NICSGAGKSHALDEFVSINDMVKTANFLLALIEHGIS"
     gene            13325..14371
                     /locus_tag="PK733_05930"
     CDS             13325..14371
                     /locus_tag="PK733_05930"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ10115.1"
                     /translation="MNKDQYIKWYSPSRKPFRIYGLPFFEMDGVYRRLQLNPPEPIPE
                     VVSDLANHTAGVQLHFRGKLRKLIVKAKLHKPANFDHMTPIGQVGFDCYLKEAGGKPC
                     FFSSTRFNHNNDSYEYELLDFPEPREFNIIINFPLYVGVHELLLGVDEDAILSEPDSL
                     ADNGRIVIYGTSVTQGACAARPGMAHTNILSRRLNREVINLGFSGSGKCEAEMALAIR
                     EIERTSLLIIETDGNCPDDAWILEKYPKFIQLYRERLPGTPILVVSRVTRPQAAIVSE
                     QFKKLRDKADAQRKVVDECAKKGDKNIYFRDGRDFLGDDFHECTVDIGHLTDLGFMRM
                     ANSLQPIIEEILGI"
     gene            complement(14484..15281)
                     /locus_tag="PK733_05935"
     CDS             complement(14484..15281)
                     /locus_tag="PK733_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ10116.1"
                     /translation="MSTNISITHDLHQHSITSFCCSDKEMIPQRLLSNAVENGYDTIC
                     ITDHVWDALVPGASKWYSEQPIEHVCQSLPLPSAEGIRFCFGCETEYCGGEKLGLARS
                     SFSLFDFVVIPINHFHMLDFVRPSSINTAILVADLLLTRLEELQQLDLPWEKIGIAHL
                     TSRLAFREGNVADVYDYMPEDRLLQIFGFFAQYGTGIELNACSFRPGWEERFNSLMRP
                     YSLAKQAGCKFYFASDAHKVSELSMMNLLHPAINALDLRAEDLYRIP"
     gene            15914..17617
                     /locus_tag="PK733_05940"
     CDS             15914..17617
                     /locus_tag="PK733_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10117.1"
                     /translation="MKYYSKVLMFVIVLSLIISAGAVGCQKTEKKTDPTAPGSTLSDA
                     KGKTSSSGETDLLNPVGTFPIAKTKTKLSLLMAQDVLVEDYETNAFTKWVEDRCNADL
                     DFVLLPAMDAKDKLAIMISSNQTLPDVVNMPLDIITTYKYGSGGAFIPLNDFYENQSF
                     YVKQQLDKYPEIDIIRFAKAADGKLYSIPQYIKELHSEVKQKLWINTKWLDKISMKMP
                     ATTDEFYKVLKAFKESDPNNNGKADEYPLVGGTGDSQDPSIFLMNSFVYDDADNRFLV
                     KDGKLSVAYATDAWREGLRYMNKLCTEKLLDPISFTQDNSQLRAMANNPDDCIVGSFA
                     FTSITLLPVATSPYINDFEGLPPLEGPAGVRFASYSPAAVLNRWFVTRDCKNPELAFR
                     VGDSLFNEEAFIKARYGVENQDWSAPKQGDISYYDEFGYKPMFVQDNNIWSSSQNSHW
                     RNNIPAFTVNVLQGGVWKDRDPLYYSRRIALTVGKYHEYKPEPGTFVPLLNLNEDEIN
                     QVSEIQATLKTYVNECKTRFIMGDMSIEKDWNSYLAEIKRIGLDKFLEVCQAAYDRMY
                     K"
     gene            complement(17750..20011)
                     /locus_tag="PK733_05945"
     CDS             complement(17750..20011)
                     /locus_tag="PK733_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10118.1"
                     /translation="MFKPKVFYRILWADLLIVLILVGMMIPLCYLMITTTRERELNDS
                     YNIMLNCIDILDSQIKSLESILTKAYVNENLVRVSIIDDKMQTSDYYAMLKAQQYIAA
                     IASSNSYVADIIVVFKSNDIILSKYTSFNSTNALNKYYHIDGMENIMFSNETEFVSGH
                     PSVLERSFNYLPETTITYLSSDPNNSRTPMEVFCYRLPLGLPMYTKENGMAYIFINKK
                     YVLDTALPQNFRENGFFQLLDNQGNIILTYGNNSISPAYKGVKYYNQFIDGTSYSIMN
                     VSNNTSTLRIVAGVPQNTFLSGMRNIFVLIGFYITLAILLGVLISLWTAYSQSAPLKK
                     LLQKLELQGFTREESYRNEYDFISTNLVRMKSENRDILKQLDAFRSTLKSNMIERLLS
                     SGQLSPIQQNDILAHLDNFPASFIVGYGEFQSQMRLDKQYSDVLKVLIIDHLKKVFPS
                     NIIYHSLGENAFALIIPVDGQGKIADTLINAIHQINAEIPAKIVLAISTAYTSISNVN
                     TAFEQSRLVYALGDKNNCLLTPSDIIHSSNSTLNIKDYLELYRYLITADFAAVEQYIR
                     QMFDFAANIPNTDMEQLYYIIRMTLLFAVRDYTGDEKVFPDIPKYQSNQSPEQMIETL
                     CNISCDICNIVNSNKRSHNSELKESILEYVKQNFSDSNVYGKQIAEHFSISEKYLYNF
                     IKEQTGYSLGDYLQYLRLELAAKLLMDSEKSIANIYNECGYTTHNTFYKAFKRVYGIS
                     PSEYRDRYIGKEK"
     gene            20405..21370
                     /locus_tag="PK733_05950"
     CDS             20405..21370
                     /locus_tag="PK733_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10119.1"
                     /translation="MTIPIQNQAGVTPRPGRKSDFMKRLRLHRQMYLFLLLPLIYLAV
                     FKYWPMLGVQIAFRRFNPTKGIWRSEWVGFANFVKFFNSYQFYRVLANTLRISLYSLI
                     AGFPIPILFALALNSLRSERYKKFIQNVTYMPHFISTVVIVGMLIQLFNTRTGIFGTL
                     YTFLTGSDAPDLLANPDAFPHMYVWSGIWKGTGWGSIIYIAALSNVDIQQHEAALIDG
                     ASRFQRVLHIDLPAIIPTITIMLILRCGDIMSVGFEKTFLMQNNLNLRTSEVISTYVY
                     KVGLSAGGGDFSYAAAIGLFNSVVNMILLTIVNWTSRRINNTSLW"
     gene            21382..22377
                     /locus_tag="PK733_05955"
     CDS             21382..22377
                     /locus_tag="PK733_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10120.1"
                     /translation="MNIATTMTDLYKNKVGDRTVKTCKTNRNNLINKNNKANRIKMET
                     SDLIYYLLVNIFLTIITLVVLYPLIFIVSASFSSPIAVSAGKVLLWPVDFSLEGYKAV
                     FKSRHIVSGYLNTFIYTGTGTAINIAMTMIAAYALSRKEMPFRGIITFLFTFTMLFGG
                     GMIPNYILMLKLGMINTRWAIIIPGAISVYNMIIARTFIQNIPNELLEAAQIDGCSDS
                     YYFFKIVLPLSKALIAILTLYYSVGHWNSYFDAFLYLSDRKLYPLQIILREILVANSI
                     PMDEIVDPETMNAKQGLADLLKYSLIIVSSVPVLILYPFIQKYFIKGVMIGSLKG"
BASE COUNT         7489 a         3600 c         4531 g         6940 t
ORIGIN      
        1 ggcctggttt tagccatgtc ccggaagata gccctggctg ataaatcagt taagactccg
       61 taatgctttt tctctggctt tttatgcacc ttcaataacc agcggagttg ctatgtcggt
      121 tgtttggctg tattttttct ctaatgaccg ctacggactt atcaataata tccttattaa
      181 tcttggaatt accagcgagc ctatattatg gacaatggat cctaaatata tattgtcagt
      241 agttattttt atttcaatat ggatgagtat gggtacgggt tttttggtat ttttggcagg
      301 cttgcaaaat gtaccaaaag agtattatga agcgggagca attgatggaa taaagaataa
      361 attccaagaa ttgtggctta ttactttccc aaccctaaaa cctcagttgt tatttggtgc
      421 catcaattcg attgtgggct cctttggagt atttgatata gcagtatccg tagcaggcat
      481 gccaagtcca aactacgctg ctcataccat tgtagctcat ttatatgact atgcttttat
      541 ccgctttcag atgggatatg cttctacagt ggcaattgta ctatttctta tgacatttct
      601 aatgggacgg cttgtaatga aaatgctctc atctaaagat atataaggaa gaggaaggag
      661 aaaattgaga atgagtaatg aaacttcaat ggaataccct gtaaaaatca tcaagcgatt
      721 aaggggaagt catagcaccc ggccaatccg ctcttataag tcaaatatga ctctacccga
      781 ggtattgctt tacatattta tgatacttct tgtaggattt acagcactac cccttgttta
      841 catgataagt acagctttca agcctttgga cgaactgttt atctttccac ccagatatct
      901 ggtaagaaaa ccaactatac agaatttcca tgacctggtt atttcgctgg gcagttctac
      961 agtacctttt gcaaggtata tattcaacag cctttatgta actgttatta ccgtaacatg
     1021 caccgtgata gtgtcatctc tgggagccta tggcctggtt aagcataaac ccaggggttc
     1081 aaaggctatt ttcaatttta ttatagcagc cttgatgttt tcacctcatg taacgcaaat
     1141 accaagatat atggtagtaa attctcttgg cttgatagat agttattggg cacttatcat
     1201 tcccaatatt gctgtggcat acaacttctt tttgatgaag cagtttactg aacaatttcc
     1261 caatgagctt ctggattcat caagagttga cggggcaaat gaatggatag tattctggaa
     1321 gattgtaatg ccgtcactaa ccccggcttg gtctactctt gtggtgtttt cctttgtgag
     1381 caactggaat gattactttt ctcctctgat ttatattacc agcagagcga agaagactct
     1441 tcccccggcc ctccaaacaa tcggagctgg agggtctatt gcaagagcag gtgcagttgc
     1501 agctgcgaca ttattaatga ccttgcctac tgtcataata tttacattca tgcagtcaaa
     1561 ggtcatggag actatgactt actcaggaat taaaggataa aggagggaaa taagtgagag
     1621 cgtttaaatc aaaaaaagca atgtcctttc ttttggtaat tgtagtggta atattgatag
     1681 tcaatagcat tgctttcgct gaaagccgtg attatattct tgatggcaat gaaagaatac
     1741 cgattccgga aacctatatt ttgaagcatg taattaacag gctggaaaac gcaggtgagg
     1801 aggttaaata tttcaatgat gctgacgacc tcttcataaa taaagaggga tacctttttg
     1861 ttgctgatac cggcaataac aggattgtta agctgaccgc cgatggaagg cttgttgctg
     1921 ttttcaaggg atctgaaaat aagcctttta actcgccaat gggtgtatat gctgatgagt
     1981 ttggcaatat gtatgtagcg gatacaggaa accaacgtgt tgttcatcta tctgccgacg
     2041 gggaatttgt tgaggagttt ttaaaacctg agtctgaatt gttaggtgct tcatatacat
     2101 tcagtccatc aaagctgtgt attagcccaa ctggttattt atatgttact aaaggacaaa
     2161 acgttatgac tatagatgca tataatcgtt ttcggggttt tatggggcaa aatgagatag
     2221 gattcaaact gcttgatgct atactcagga tgtttgcatc tcaagaacag aaaagagcaa
     2281 ttgccaagcg tacagcagca gcatacttaa acctgacgat ggatgaaaaa ggtatgctgt
     2341 atgcagtaac tatggattcg gcagatgggg aaattaaaaa gctaaactct attggtgaga
     2401 acatctaccg gaaatatgga ggaatacaac aggatatgaa gttgctaagc ctggatttta
     2461 ttacaaaaat gaaattaaaa agtacgagtt ttgtattcgg tgaaagaaaa gacgaagaag
     2521 gaaaatcaat taagcctttt ttcaaagata ttgcagttga caaaaatggt gttgtaactg
     2581 taattgaaca gcagactgcg aaaatatatc aatatgatca ggaaggtaat ttattaacgg
     2641 tgttcggggg taaaaaagcc caaaaaggtg gtttctccat tccaacatct ctggcagtgg
     2701 atccacaagg gtgtatttat gtactggacc gggggttagg aaacatccag gtatttgagc
     2761 ctacaagatt tattaaatta gtacataacg cagtaaaatt atattctgat ggagattatg
     2821 acagatcata tgctgcctgg caggaagttc taaaaactca tgagaactac cggcttgcac
     2881 atgaaggcct tgcaaagact cttttcaaac aaaaaaaatg gaaggatgcc atggaagagt
     2941 ttaaagaagc tgatgacaga aacggctact caaaagccta tattgaatac cggtattata
     3001 ttttccgtaa atactttaca ctggtggttt tatgtattgc ggccattgtt gttggattaa
     3061 tatatttcat taaaggtatc cgcaagttat cggaaagcgg cctggagcaa tttagaaatg
     3121 gaggaactaa actcttcagc atatgtgacg ttctaaagtt ttcaacagca ataatattcc
     3181 atcctgttga aacttttgaa ctaataaaga gcaacaggga gcgtataagt gttaagcctg
     3241 gagtgataat tcttttactg gcgtttgttt ccagaatatt atatatgttt gttgtgcatt
     3301 atcctctggc agatattgat ttgcgtgatt ccaatattat acttgaagcg gttaagttat
     3361 tgcttccggt agttacctgg gttgttgcca gttttgccat tactgcaatt acagacggtg
     3421 aatcaaaaat aaatgagata tttattgcat cttctttctg catgatacca tatattttga
     3481 taaacactcc attagccctg ttatcaaata tattatcaag gggtgaacaa acgttgtatg
     3541 gatttattca aaatggcaca tggatttgga tattgattct gttctttatt caggtaaaaa
     3601 tactaaatga ttataaaaca cctaagacaa cagcaacttg tgtagtgagt gccctgaata
     3661 tgggccttat atgggctgtt tgtctgatga tttatgtttt atcaggcaga ttggttgaat
     3721 ttattacagg tattattcag gaaataaaga tgatagttat ttgaaaggag ggggtattgt
     3781 ataatgttgg ttaaaaaaca aaatggaaat aaagcattgg ttcttatagt gagcttagca
     3841 ttattactga cagcaaaatt tcccttaaag agcgaagcag ctcctttaaa tgtacaacta
     3901 agcggcgaat atgtagaagc gttgaaaact gaaagccttt tactgtactg caatttgaat
     3961 acaggagcga tagcagtatt tgacagaaga aatgggcata tgtggaaatc cacgatagac
     4021 attgaatctg atgagggtgc ttttaaaaat gttggtgtat cctggaagaa atccatgtca
     4081 tctctttttg caattagtta tattaatatg aataaaaatg atgtaattat agaaaaagca
     4141 ttttctactt cagatgcaga cgatataaaa gttacctttc ctaataataa agtaatgatg
     4201 gaatattctt ttggcaagca gggaatcaaa gtagcggttg aaataatgct ggagggggac
     4261 aatttagttg tgaaagtccc tgtagataaa attgaagaaa atgatgtata cgggttgata
     4321 gcattagaac ttatgccatt tttcggagct tctgacagaa gtgtagatgg atacattttt
     4381 taccccgacg ggtgcggagc tattatgaag tttgaaaatg ttgaaaaacg tcctaaagat
     4441 tcaaagcctt ataaggcatt tatttacagc cctgaagagg taagtttgaa ggaatatgga
     4501 caaatgaaaa aagatcagca atatcaggcg tcattacctg tttatggtat aaaaaatgga
     4561 gataatgctt ttcttgctgt tgtaacaaaa ggagaggaag aaacagctat tcaagtaagt
     4621 cctgagggct atgcagttaa cttgaactgg gccggtgttg agtttaaata taggcatttt
     4681 tataatctga tactttctaa tattactatt aatggaaagg acatagctaa aaaaactaca
     4741 agcagcaaac cggataaaga gttagtattg caggatcatg aagtgcgttt tatgtttatt
     4801 gcaggtcaaa acgcaaatta cagtggaatg gctaattcct atagagagta cttaattaaa
     4861 aataatcaat tgaaacaagc tgtaaaacct gatggcaata taccattggc tctggatatt
     4921 tttatgggta taaaggaaaa aagaatgcta tttgataagt ttattaccat gacaagcttt
     4981 gagcaggcaa aaattattac ggaaaaattt ttgagcaatg gagtaaaaga gctacaaata
     5041 aacttaatag gatggagcaa aggaggatat ggagcttatc ctgttaactg gccgccggaa
     5101 cgtctgctgg gcgggatttc agggttaaat aaatatcttg gatacacaaa ggaaaataat
     5161 gtaagtgttt ttttacaagc cggttttctc catgcccttg gggataacgg agggttttcc
     5221 caaaaaaatg atatagctat acaaggaaat agtcttcctg ttactaatat tgataaaaat
     5281 agatttattt taaatccatt ttccgccttt aacaggttca cagaattcct gaaaaaactt
     5341 aatacctttc cctacgcagg agtggcgctg gaagatttgg gggagtttgt atatcatgat
     5401 tataataaaa agcagccatc cggaagaaat gatacagtac aaaaatggaa ggatattctc
     5461 aatgcttctc tcatagagga aaagccattg gcagtaggcg gcggcaatgc atatgtactg
     5521 aaatatgccg acagactgtt taatatacca gttaatagtt caaactacca tattaacgac
     5581 gaaaccattc cattttatca aatggtggtt catggaatga taccatatac ttcagaaccg
     5641 ggtaatttat ttttcgatcc tgtaaaacaa aagctgcagt gggttgagta cgggtgtatg
     5701 ccttattttg agctgactta tgaaagtgca attcaattga agcatacaaa atataaccgg
     5761 ttgtttaatt caaattacga aagctggctt caaactatta cagaaatata tgaagaattc
     5821 aaccagagac taaaaggaat atggtctgaa aaaatggcag aacataaaaa agtaaatgac
     5881 aaactggtga aaatccgcta tagtaatgga accactattt acatcaacta tgatgaaaaa
     5941 gaaacatatt atgatggtta tgtaattgaa gcacaagatt atttaatagt ggagaaagga
     6001 agtggatttt aatgaaaaaa ttccggattg gagcagaaaa gagaaatatt cttgaaggat
     6061 acatctttgt cttgccattt attctgggaa cattaatatt ctttgcattt cctttgtata
     6121 tgtcaattaa gctaagtttt ggaaaattac agaggataga gggctttata ataagatgga
     6181 taggatttgg taattactta aaagcattta ttactgatac taaattcatt cccatgtttg
     6241 ttggcgtgtt tcgtcataca ttaattaaag caccattgac aattatattt gcattaatta
     6301 tagcaatatt aataaataaa aatgtgaaat tcaggggctt ttttagaaca gtattcttta
     6361 ttccgttttt actgggaatg ggagaagtaa tgaagcagtt attaggtcag ggggttgata
     6421 ggcaggttgt ttctatttca ggtgggagac ttttacccag ggaattgctg atgtacttgg
     6481 gtcctaaggt tgtagaagca attgacagct tttttggtat aataatagtt gtcctatggg
     6541 gttcgggggt tcaaatacta ctatttctgt cagggctcca gagtattccc gcttcattat
     6601 atgagtcttc caaagtggat ggagcaactg aatgggaaat gttttggaag atcacttttc
     6661 caatgatatc tccaatttta ctcttaaata ttgtatatac attaattgct tcatttaccg
     6721 acataaccaa tccgatactg gaatacattc aaaatcttgc ctttaaaggg atggagtttg
     6781 aatatgcagc aaccatcggg tggatttatt ttgtttttat tataatattg gttatgctgg
     6841 ttttcatttt gcttaggaat tatgtatata ctactgatgc aaaggaggct aaaagaaatg
     6901 acagaagaag cagctactaa aaaatggaga acttctaata ataagacaca atattcgatt
     6961 cccggattca taaggaatat gcaggaaagg atggcggaaa gaagagcatt aaaactgagt
     7021 gatagagtgt ttagaatagg aacatatatc ttgctttttg atctggcgtt tgtttttctc
     7081 tttccgtttt tttacatgat agtaacatca ttaaaaacca atgcggattt gtacaatttt
     7141 actgttaact gggtaccaag aactgttaaa attcaaaact atgttatagc ttttagagct
     7201 cttgattata taaagtacct taaaaactcc ctgatattga cattgctagg gactgcagga
     7261 catatacttg cgtgttcatt tatagggtac gggtttgcaa ggtatgattt cccgggaaaa
     7321 acattgcttt tttccatagt tattttaagt cttattgttc ctgtccaaac aattataatt
     7381 cctcaatata tgatattttg taacctgaag tggcttaata catatcttcc aatgatagtt
     7441 cccacatttt tcggttatgg gttgaaaggt ggtctcttca tatttatatt ccgccagttt
     7501 tatctgggac ttccaaaagc attggaggac gctgcgaaaa ttgatggatg cggttttttc
     7561 agaacttatt ggaatatagt actaccagta gcccaatcgg ctttactggt gactttggtg
     7621 ttatcattgg tatggcattg gaatgacttt tatgaacctt ccatctatat aagcaagatg
     7681 atgaagactc cattgccttc caggcttaat gaactggtta atttagtaaa tactcctccg
     7741 tcagaggaga tttttgacag tttgattgaa ttggaagcca tggaagaaga gatggctatc
     7801 aataatgccg tactgatggc cggaacattt cttgtggtga ttcctgtaat gactgctttt
     7861 gcatttcttc aaagcaagtt tatgcaagga attgagcgca ctgggcttgt agaataaagt
     7921 aataaaatat atacttgaca aaaaaaataa ctttgaggac aaaaaaggta gtttaatata
     7981 atactgcatt gatgtatgct ttattataag gaaattgtac tatgctgcag caggtataat
     8041 tgtatctaca acaatatcaa taactaaaat tatacaacta ggtatttgga cacgtcaata
     8101 acaaattttg atctttgaag gaggtatttt aatgaaaaag agtttcacaa ttatgctggt
     8161 attaatgatt ttacttacat catttacagg ttgtgtgaaa aagaccgatt caaatgatgc
     8221 aaatgtacaa acgagtggga agagcagtga aaaacccgtt caaagcacga aagaggtaag
     8281 caattttaat cctgtaggat tacctatagt aaatgaaaaa ataactttaa aaatgatggg
     8341 ctcaagacat tctgcacaag gaccttggga agatactata gttatgaaat ggcttgagga
     8401 agaaacgaat attcatatgg atgtttattc agtaccaagt gatgtatatg ctgaaaagaa
     8461 aaaccttgcc tttgccagcg gcgacctgcc agatattttt ttcaaagcaa ctttgagtgt
     8521 taaagatgaa gaggcatatg gctcccagaa tctacttgtg tcgctaaatg acttaattcc
     8581 caaatatgct cctaattttt caaagatatt aaaagaaaaa ccacatgtta aaaaggctat
     8641 ttcgtctatt gaagggaaaa tttattctct tccctatgtt tattatacca attccatggc
     8701 aaatgtccgc tacatcaaca ctaaatggct ggaaaatgta ggtatgaaaa taccggagac
     8761 cattgatgat ttttaccagg cattaaaagc ctttaaagac atggatccaa atggtaataa
     8821 ccagaaagat gaaattccaa tgacggatgt aaatatcggc ggtgcatcat tctatttgct
     8881 tccggcgttt ggattcgtca ccagtgccgg taaccgatgg gatgctaaag ataacaaggc
     8941 agtatatata cctttacaaa atgagtataa agaattcata atatatatga gaaaattata
     9001 tagtgaagga cttttagacc agaatatatt cactcataaa acagcagaac ttgatgccaa
     9061 attgggagaa tacagagttg gtgtgatgaa tgacagcctg gccaaaatac ctgaagaaaa
     9121 acaggatgaa tatagagtgc tgcctccttt gacttcacat cttaatagtc agaaaatgac
     9181 atatttgatt ccagggtatg taacaggaac ttatgctatt acaaataaaa ataaatatcc
     9241 tgaagcaagt atgagatggg ttgatttatt ctattctgaa cctaaagatg caataaacgg
     9301 gatctgcgga ttcacaatgt ttaggggtaa agaaggagag cattgggttt atgagaatga
     9361 tgaaagaacc ctgtttagtc atatacccct tccggatggc aagagcggag attattattt
     9421 atcgccattc agaaatggag gagcgccaat gtatatcacc tccgcactga gtaataaaaa
     9481 ttctttacta tcaacaagaa taaaagttga aggctcagca gaacactact ttccatatat
     9541 gaaccgagta ggctatccag gtctaaggta tactgaagat gaaaatgaga aaaagaactc
     9601 catagaagat gagcttggaa agtatgtaag ccaaatggaa gtaaaattta tccttggaga
     9661 agaacctata gagaattggg acgctttcat tgaaagaata aagagcattg gtatagatga
     9721 agtaataaaa atcagacaaa ctgctttaga tagatggaat agcttctaat cataaaaaag
     9781 ttttggtttt taatgcagtg catttataca agaatcatat ataaagtacc tagtgggtgc
     9841 gccggataaa actggcgcat ctactatgat aaatcagatc acggacagca tgaatacaaa
     9901 aaagataact gtttagttgt cagaattgca agtatgggag gttattataa atatgtccgt
     9961 tattcaaaga gtattaataa tggtattagc agtgttagtt atatttggtt ctttaatagg
    10021 atgtaataat tctataccaa acagtcaggg aaaacaaact actttagacg gaaaaaaccg
    10081 ggaaaatgca aataataaac tggcattgga aaacggggga tttgttatgt atgatgatac
    10141 ttcgaagaat attaaaatag aaaacgaata tataagtttg gaaattaatg atgaggggaa
    10201 attattagct ttcacagata aaatatcagg taatagctac ctcaataaag aagagtactt
    10261 tgttatggca gctaaaggca gttttaataa atattttccg gtaagggctg cttttgaaaa
    10321 cggaaatcta aagactataa gcacagaagc agtcaatgaa ggcaagatta gatttgaact
    10381 ggcaaacagc gaaggtgata aagttggagc agtagtaggg attaaagtat ataatcatta
    10441 catttctttt gaaataaatg agattaccaa tcaggattct attaatgata ttacttttgc
    10501 gaatttaaat acaaatatta acggtacggt gtcaacaaaa gttaatgcct gctataataa
    10561 tgattttgca gtttgtgtaa tggctcttac acttcccgtc cattgtgctc cggagactgt
    10621 cgatagctca actattctta atgcaaaatg ttactctgaa tttggtatta aaggaaataa
    10681 atttgctatt attgccaccc catttaagac cttcttagaa accgtacagg aggttgagga
    10741 gaatgaaaat cttccttcac ctaaattagg cggtaaatgg ggtaagctgt ccgatgatgt
    10801 acgaaaatca tacttattta tccgtaattt gacagaagaa aatgctgatg atgtcataag
    10861 atttgttaag gaaatggatg caggaatgat tttatttggt tcagttgtat ggagagcaac
    10921 agcgggacat tataatatca ataagcgaag tttccccaat ggtgttgaag ggatgaaagc
    10981 agtggtcgat aaaatccaca gtgtcggtct taaagcagga ttacacttcc ttcctgctga
    11041 aatcaccgtc agtgaccctt atgttacacc tgtgccggat aaaaggcttt ataaagatgg
    11101 tgtagctgtt ttaactgctg atatatctaa tgaaacaata acaatagact gttcggaaga
    11161 aaaatctgca aacagtgcat ttcctattaa agtaattgat gcttatcggg aaacagggac
    11221 tgatgtattg attgatgatg aaattattca ttataaaatt aaaaatatta atagaagtgc
    11281 cgcaggaaag gttgaaatta ctttagaaga ttgtgtacgc ggagcatatg gtacaaaaaa
    11341 agcttctcat aaatctggaa caaaaatata tcatttaaga cgtgtgtatg gaaactttca
    11401 aattgacctt gaaactactc tgcttgatga ggtcagcaca agaatggcag atatatttaa
    11461 ttattgcggc tttgatatga tttactttga tggatcggaa aagcttcagc agcttccttc
    11521 atacacaagt ccaaacacaa atcattggta ttataatgca aaactgcaat atacatttta
    11581 taacaagctt gaccgtaaag aaaatattct tgttcaaggc agcagtaatt ctcattttgg
    11641 ctggcacatt atttcacgac acgcaattgc tgatgggaaa aaggatatta aaagatcatt
    11701 agattctaat atgggaaaaa tagaagcaaa taaccgggat tttataccta ttgatattgg
    11761 ctggtattca attggagcat cacacatttc atatgaagat attgaataca tattgtgccg
    11821 ctctattgga tttaattctt cagttgggtt tcaggaatca tcaagtatag ggtctagaat
    11881 cgcaaatgat atggttgaac tgattaaaaa gtatgaaaag ttaaggcttg aaaattattt
    11941 ccctgaagag gttaaggaaa tgcttagaaa accgggcata gattataaat taatacagga
    12001 ttcgcaaggc cgctggattt ttgtgcaatg attatatgaa aggtattctt gaaccattgt
    12061 atgctattga gctgttgaat agcttgaata attgcaatat aatgtaaacg aggtgttata
    12121 attggtaaat aaagaaagaa tggtaaatga atttattgag cttgtagaaa ttgacagcct
    12181 gtcatataaa gaaagagata tggcagatag attaaaaaat aagcttactg caataggata
    12241 tgatgtttac gaagatgatg cagggaaaaa ggtagggggc aatacaggaa atctcatatg
    12301 taaaattaag ggaaacaagg atgtgcctgc tataatgcta gtagcacata tggatactgt
    12361 tgtacctgga attgggaaga aagccgtaat taagggagat gttatagtat ctggtggagc
    12421 aacagttctt ggtgctgatg atatatcagg tattgagtgt attttggaag ttgtcagagt
    12481 cttgaaagaa aagaatataa gccatggaga tatttatgct gtgtttacaa tagcagaaga
    12541 aaaaggcctt ataggagcaa aaaatcttga ctatgaaaaa atagatgcta aatacggatt
    12601 tgttctggat gtaggcggga aaagcggtaa tgtagcaata agacaaccaa taaagcatgc
    12661 cacaacaatt actgtactgg gtaaagcagc acatgcaggt tcaagacctg aagaaggaat
    12721 aaatgcgata cagattgctt cgaaagcaat agcggggatg aaacttggaa gaatagattc
    12781 tgaaacaact gccaatatag gtattataag aggcggggag gcttcaaata tagtatgtga
    12841 cagggttgat ataaaagccg aagcaagaag cctgaataaa gaaaaattag aaactcaaat
    12901 aaatcatatg aaagaatgtt ttctttctgc tgcaaaagaa tacggcggca gtatggaatt
    12961 tgagagtaaa cagaccagtc ccggttttag tatggatgaa aatgatgaaa taataaaaat
    13021 attaaaaaag acagctgaac tcacaaattt caacctgaat ctgatatcta caggcggtgt
    13081 aagcgacact tgcatattaa acagtaaagg tattcaggca gttaatattt gttcaggagc
    13141 tggaaagagt catgcccttg atgaatttgt aagcataaat gatatggtta agacagcaaa
    13201 cttcctattg gccttaatag agcacggaat ttcctaggct gcaataaata taggattaac
    13261 ctctaactct atagtagagt aaaatatttg tactttaaat gaaatgaaag gaatggtata
    13321 tattatgaat aaagaccaat atattaaatg gtactctcct tcacggaaac cttttagaat
    13381 atacgggttg ccattttttg aaatggatgg ggtatataga cggcttcagt taaaccctcc
    13441 ggagcctata ccagaagtag taagcgatct tgcaaatcat acagccgggg ttcaactgca
    13501 ttttcgggga aaactccgaa aacttatagt gaaggcaaaa ctgcataaac ctgcaaattt
    13561 tgaccacatg acacccatag gacaggtcgg ttttgattgc tacctgaagg aagcaggagg
    13621 aaaaccatgt ttcttttcat caacgcgttt taatcacaac aacgatagtt acgagtatga
    13681 attgcttgat ttccctgaac ccagagagtt taacataata ataaactttc cgctatatgt
    13741 gggagtgcat gagttgctat taggtgtaga tgaagatgct atactttctg agccggattc
    13801 attggctgat aatggccgta tagttatata tggaacttcc gtaacgcagg gtgcatgcgc
    13861 agcacggcca ggtatggcac atacaaatat tttatcacgc cgtttaaatc gggaagtgat
    13921 aaaccttggc ttttcaggaa gcggcaaatg tgaggcggag atggcactag ctatccgtga
    13981 aatagagcgg accagtcttt taataataga aacggatggg aactgtcccg atgatgcatg
    14041 gattttggag aaatatccaa agttcatcca gttatatagg gaaagattgc ctggcacacc
    14101 aattttggtt gtatctagag tgacgagacc tcaagctgca atagtgagtg aacaattcaa
    14161 aaagcttcgt gataaagctg atgcacagag aaaggttgtt gacgaatgtg caaagaaggg
    14221 tgataaaaat atatatttcc gcgatggacg ggacttccta ggtgatgact tccatgagtg
    14281 cactgttgat attgggcatc ttacagattt aggatttatg aggatggcaa atagtctaca
    14341 accaattatt gaggagatat taggcatata aacttatatc atttacttat aaaaattctg
    14401 gaaagtgaga taacgttttt catatgagcg ggtaaacaag ctaattaagc aatgattacc
    14461 cgctgatttt ttatgcaact ttcttaagga attctatata aatcttctgc ccttaaatct
    14521 aaagcattta tagccggatg caatagattc atcatggata attcagaaac tttatgggcg
    14581 tcagaggcaa aatagaactt gcacccagcc tgcttagcca agctatatgg gcgcattaaa
    14641 ctattaaatc gttcttccca tccaggtcta aatgagcagg catttaattc tattcctgtt
    14701 ccgtattgcg caaaaaagcc aaagatttgt aaaaggcgat cttcaggcat ataatcatat
    14761 acatccgcaa cgttaccttc tctaaatgcc aaacgagatg taagatgtgc aattcctatc
    14821 ttctcccagg ggagatccaa ttgctgaagc tcctccagac gagttagcaa aaggtctgca
    14881 acgagtatgg cagtattgat ggacgaagga cgtacaaagt ccagcatatg gaagtgatta
    14941 atcggaatca ccacaaaatc aaacaagcta aatgacgacc tggccaaccc cagcttttca
    15001 ccaccacaat attcggtttc acaaccaaag caaaacctga tgccttctgc tgatggcaaa
    15061 ggcaatgact gacatacatg ctcaataggc tgctctgaat accattttga agccccggga
    15121 acaagtgcgt cccagacatg atcagtaata caaatggtat cataaccgtt ttcaacagca
    15181 ttggaaagca atcgttgagg tatcatctcc ttatcagaac aacaaaatga tgtaatactg
    15241 tgttgatgaa gatcgtgagt aattgaaata tttgtagaca tagagccact cctttaaatt
    15301 gaaaattata agaaaataaa gattaacaaa taagcaagac aaaaaccaga tttatttatt
    15361 catttataca tgaaaatatt tgtacttttg cttttatttg ttttatttta ttaagtatat
    15421 aacacaaact tccaggtgta aagggtaaaa aattgctcac tttaataata aagtatgaaa
    15481 tactttactg aattaatagc atgaatatga aagttgaaag aatgattttc taaagcgcat
    15541 ctatacagca caaaaaaatg catccatgtg caagtatatg caaatatgaa agctattagt
    15601 ataaattgca gcattatact gttttataaa cgttgcgcac cacttactta ttcaatataa
    15661 tcacgacaga aatctcgcat accaaggttg taccaagcac catattacta taatacaggt
    15721 tgtgcagaaa aatcaattag ctatttcata ctattatacc taacctgcca aaatatcaca
    15781 tttacatgtc tgcaatgatt gtggcatcat atacatagat taaagctgcg ataaactgat
    15841 tgattttgca gctgcaatct ataacttatc atactacatt acttaatatt tattaaggag
    15901 gctaagtaaa aagatgaagt attactcaaa agttttaatg tttgtcattg tattaagttt
    15961 gattatcagt gcaggtgcag ttggctgcca gaagactgag aaaaagacag acccaactgc
    16021 tccgggttca acattatcag acgcaaaagg aaagacttcg agttcaggtg aaactgattt
    16081 gcttaatccg gttggtacat ttcccattgc taaaacaaag acaaagctgt cattgcttat
    16141 ggctcaagac gtgcttgtag aagattatga aacaaacgct tttacgaaat gggttgagga
    16201 cagatgcaat gcagacctgg attttgttct tttgcctgcc atggatgcaa aagataagct
    16261 tgcaattatg atatcgtcaa accagacatt accggatgtt gttaatatgc cgcttgatat
    16321 aataacaaca tataagtatg gaagcggcgg tgcatttatt cctctgaatg atttttacga
    16381 gaaccagtca ttctatgtta agcagcaatt agataaatat ccggaaatcg atattataag
    16441 atttgctaaa gcagctgatg gaaaattgta ttcaattcct caatatatta aggagcttca
    16501 tagtgaagtc aaacagaagt tgtggattaa tacaaaatgg cttgataaga taagtatgaa
    16561 aatgcctgct actacagatg aattctataa agttttaaaa gcatttaaag agagtgatcc
    16621 caataataat ggaaaagcag acgagtatcc tcttgttggt ggtaccggcg attcacagga
    16681 tccaagcatc tttcttatga attcttttgt gtatgatgac gctgataacc gtttcctggt
    16741 taaagatggc aagctcagtg tagcctatgc aacagacgca tggcgcgaag gtttgcgata
    16801 catgaacaag ctatgtactg aaaaattgct ggatccaata tcatttaccc aggacaattc
    16861 tcaattgcga gctatggcaa acaatcctga tgattgcata gtgggaagct ttgcttttac
    16921 ctctattaca ttgcttccgg tagcaaccag tccatatatt aatgattttg aagggcttcc
    16981 tccccttgaa ggtcctgcag gtgttcgttt cgcatcatac tctcctgcag cagtacttaa
    17041 tcgctggttt gttacacgtg attgcaaaaa cccagaattg gcatttcgtg ttggagattc
    17101 attgtttaac gaagaagcct ttataaaagc acgctatggt gtagagaatc aggattggtc
    17161 agctccaaaa cagggtgata ttagctatta tgatgaattt ggctataaac cgatgttcgt
    17221 acaagataat aatatatggt cttcttctca gaacagccat tggcgcaaca atattcctgc
    17281 ttttactgta aacgttttac agggcggagt atggaaagat cgtgatcctc tttattatag
    17341 ccgccgaatt gctcttactg tgggcaaata tcatgagtat aaacctgagc cgggaacttt
    17401 tgtgcctcta cttaacttaa acgaggatga gataaaccag gtaagtgaaa tacaagctac
    17461 tcttaaaaca tatgtaaacg aatgtaaaac ccgctttatt atgggtgata tgagcattga
    17521 aaaggattgg aattcttatc tggcggaaat caaacgtata ggattggata aatttcttga
    17581 ggtatgccag gctgcatatg atcgcatgta taagtaataa atatgtaaca taatttactt
    17641 aacggcatcc ttgctggact acataagctc atactttaaa tcaaaatcaa taaaagctaa
    17701 tacaggctaa tacagaaact atacacatac ccgccacttt cgtggcgggt tatttttcct
    17761 tcccgatata tcgatctcta tattcactgg gtgatatgcc ataaacccgt ttaaaagctt
    17821 tataaaatgt attatgtgtt gtgtagccgc attcattgta tatattagca atactttttt
    17881 cagaatccat gagtaattta gcagcaagtt ccaatcttaa atattggagg taatctccaa
    17941 gactgtaacc tgtttgctct tttataaaat tatatagata tttctccgaa atagaaaaat
    18001 gttctgctat ctgcttgcca taaacattac tgtcgctaaa gttttgcttg acatactcca
    18061 gaattgattc cttaagctca gaattatgac tgcgtttatt actattgact atattgcaga
    18121 tatcacagct gatattgcat aaggtttcta tcatttgctc cggactctga ttggattgat
    18181 atttaggaat atcagggaaa actttctcat cacctgtata gtctcgtaca gcaaaaagca
    18241 gcgtcatgcg aattatataa tagagctgct ccatatctgt attaggtata ttggctgcaa
    18301 agtcgaacat ctgccggata tactgttcaa cagctgcaaa atcagcagta ataagataac
    18361 ggtacaattc aaggtagtcc tttatattaa gtgtagagtt agatgagtgt ataatgtcag
    18421 atggagtgag aaggcaatta ttcttatctc ccagcgcata aacaaggcgt gattgttcaa
    18481 atgctgtatt gacattactt atactggtat atgcagtgct tatagcaagt acaatctttg
    18541 cgggtatctc tgcattgatc tgatgaatcg catttatcaa agtgtctgct attttacctt
    18601 gtccatcgac tggaatgatt agcgcaaagg cattttcacc taaactatga tagattatat
    18661 ttgaagggaa tactttttta aggtgatcga taatcagtac tttcaaaacg tctgaatact
    18721 gcttatccaa tcgcatctgt gattgaaatt cgccataacc aacaatgaaa ctcgcaggaa
    18781 aattatcaag gtgtgctaaa atgtcatttt gctgtatggg tgataattgg cctgaactta
    18841 aaagcctttc aatcatattg ctttttaaag tgctccgaaa cgcatccaat tgttttaaaa
    18901 tgtctctatt ttctgatttc atgcgtacca ggttggtact tataaaatca tattcattcc
    18961 gataactttc ttctctagta aagccttgaa gctctagttt ttgcaatagc tttttcaatg
    19021 gggcagattg gctataagca gtccaaagag agataagtac gcctaaaagt atggctaaag
    19081 taatataaaa tccaattagc acaaagatat tccgcatacc tgacaaaaag gtattctgag
    19141 gaacaccggc aactatacga agtgtgctgg tgttattgct tacattcata attgagtagc
    19201 ttgtgccatc aataaattgg ttatagtact ttactccttt gtaagcaggt gaaattgaat
    19261 tattgccata agtaagtata atgtttcctt gattgtccag aagctgaaag aatccatttt
    19321 ctctgaagtt ttgcggaaga gccgtatcaa gtacatattt tttattaata aaaatatatg
    19381 ccataccatt ttctttagta tacataggca aacccaatgg tagcctataa cagaagactt
    19441 ccatgggtgt cctgctgtta ttgggatccg aagaaagata agtaatggta gtctcaggca
    19501 agtagttaaa ggaacgttcc aacacggacg gatgtccgga aacaaactcg gtttcattag
    19561 aaaacataat attttccata ccatcaatgt gataatactt attgagggca tttgtgctgt
    19621 tgaagctggt gtatttggac agtataatat cgtttgattt gaatacaaca attatgtcgg
    19681 caacataact attggatgaa gcaatagcag cgatatactg ttgcgcttta agcattgcat
    19741 agtagtcgct ggtttgcatt ttgtcatcta ttatactgac acgaacaagg ttttcattaa
    19801 catatgcttt ggtcaatatg ctttccaaac tctttatttg gctgtccagt atatcaatgc
    19861 aattaagcat tatattatag ctgtcattca attcacgctc acgagtagtg gtaatcatta
    19921 gataacacaa tggaatcatc ataccaacga gaatgagaac tatgagtaaa tcagcccata
    19981 atatgcgata gaagaccttt ggcttgaaca tgtgtgatta attcctccct aatgtaatgt
    20041 atgattaatt cttcctggta catatagagt tgtacaatct ttacaccaat tgtagcctaa
    20101 acattaaaaa gaagtcaata gtacattttc acaatatatt atgtaaatat tatctgtaaa
    20161 agtcatcctg aattgttctt actattttca tactgatgcc ggcaattcaa acaaggattt
    20221 cttactgcat taatgtgact gtcattattt caactttaca tatgggtatt ccggctatat
    20281 attttaatta tgttttgcat atggactaaa tttgtgcaag caaacttgtt tagcttgaga
    20341 tcaaaattat gttcaaacaa aattcaggta cttattatta actatattga tctggaggga
    20401 tatcatgacg attccaatac aaaatcaagc tggggtgaca ccccggccag gaagaaaatc
    20461 tgattttatg aagaggttga ggctccatag gcagatgtac ctttttttgc tattacctct
    20521 aatatattta gctgttttta aatattggcc aatgttgggt gttcaaattg cattccgtcg
    20581 atttaatcca acaaaaggga tatggagaag tgaatgggtt gggtttgcaa attttgtaaa
    20641 attcttcaat tcttatcaat tttatcgtgt tttggctaat actctgagaa tttcgcttta
    20701 ctcactgata gccggatttc cgataccaat cctttttgca ttggcactaa actcattaag
    20761 gtcggaacgt tataaaaaat ttatacaaaa tgtcacctac atgcctcact ttatttctac
    20821 tgttgtaata gtgggaatgc ttatacagct tttcaataca cgaactggca tttttggtac
    20881 tttatatact tttttaaccg gcagtgatgc acctgacctt ttggctaatc ctgatgcctt
    20941 tcctcatatg tatgtctggt caggaatatg gaaaggtact ggatggggtt caattatata
    21001 tattgcagca ttgtcaaatg ttgacataca gcagcatgaa gcagctttaa tcgatggcgc
    21061 aagccggttc cagagagtct tgcacataga ccttcctgct attataccaa caatcaccat
    21121 tatgttgatt ttacggtgcg gtgatattat gagcgttggc tttgaaaaaa cttttttgat
    21181 gcaaaacaat ttgaatctgc gtaccagtga agttatatct acctatgtat ataaggtggg
    21241 cctttctgca gggggcggcg acttttcata tgctgcagca ataggcctgt ttaattcagt
    21301 agttaatatg atattgctta caattgttaa ctggacttca cgacgaatta acaatacgag
    21361 tttatggtaa aggagaaaac tatgaatata gctacaacta tgacagattt atataaaaac
    21421 aaagtaggtg acagaaccgt taaaacctgt aaaacaaaca gaaacaattt aatcaacaag
    21481 aataataaag ctaatagaat taagatggag accagcgacc tgatctatta tttactggtg
    21541 aatatttttc ttacaataat tactttagta gtgttgtatc ctttaatctt cattgtatca
    21601 gcttcatttt cctcgccgat agcagtttct gcaggcaaag ttcttttatg gcctgtagat
    21661 ttcagcctgg agggttataa agcagtattt aaaagccgcc atatcgttag tggttactta
    21721 aatacattta tctatacagg gacaggtaca gccattaata tagcaatgac aatgatagcc
    21781 gcctatgcac tatcgcgtaa agagatgcct ttccgtggta ttattacctt tcttttcact
    21841 ttcaccatgt tatttggcgg aggcatgatc ccgaattata tacttatgct aaagcttgga
    21901 atgatcaata cccgctgggc tattatcatc ccgggagcta tttccgtcta caatatgata
    21961 attgcgcgca cgtttataca gaacattcca aatgaactgt tggaagcagc acaaattgat
    22021 ggttgttcag attcatacta tttctttaaa atagttctgc cattatctaa agcccttatc
    22081 gccatattaa cgctttatta ttcagtgggg cactggaact catacttcga tgcattcttg
    22141 tatttatctg accgcaagct ttatccgctt caaataattc tccgggaaat attggtagcc
    22201 aacagcattc caatggatga gattgttgat cctgaaacta tgaatgccaa gcaaggactg
    22261 gcagatttgc tgaaatactc gttgattatt gtatcaagtg ttcctgtatt aatattgtac
    22321 ccatttattc agaagtattt tataaaaggt gtaatgatcg gttccttgaa aggataaagt
    22381 actatactct ttaacatatt gtacgaaagg atgcacaaaa tatgaattaa cctcttgaca
    22441 tattgtacaa tactatgtac aataatatca ggaggtgttt tttatgttag ttgctgcaag
    22501 tgaattgaga aataatttta gtcattattt gacacaggtt acagatgaaa ataaggaggt
//
LOCUS       DAOSDD010000045        22096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1026_length_22096_cov_5.319359, whole genome shotgun sequence.
ACCESSION   DAOSDD010000045 DAOSDD010000000
VERSION     DAOSDD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22096
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_22096_cov_5.319359"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            604..3564
                     /locus_tag="PK733_05960"
     CDS             604..3564
                     /locus_tag="PK733_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HOJ10121.1"
                     /translation="MKKTLVCLVFLVLVLFIYPSIALSNSPPLIMKGDTAFPISSGSI
                     KLESEVIKIHYGITEKHGALGHEIEVIFNFHNTSSETDLDIGFPNVASYAQKLRDFEA
                     YDYPSMTPYETEIKEGGTLPEHDLYDYNSMYTWKMHFEENEKKSVYVKYRFESQQWGA
                     DYILVTGALWKDRIDKIDVYVDFPEPAAFAQITASPLNYYYNGKGIEWHFENIEPDFN
                     LFIGFVDLPEFYEKKDEYFRPRWNDPMDTYRWDDCFYYIDLFSSYEDMGRIALVYNKD
                     IDYIRRVVERLKDTNQFVINEIYARHGYDFKTKKWEKIFEDTGWYKHDPGFTPDVFNS
                     MEHATIAYINCFNKTVITDGSDSELIDSTNEFQDKYNGKDIFYRGMTYPYYQYTYNLV
                     SSEKERIKRIDENNGPFLSLGGQPKGLSEQLDGEITGDYPASEKPIKTLDVSDTRKIE
                     LYENGIFVRDGKDSKCIFRYQTFLKTEDLGKHNYTDPIESVPWINDDISASPMGNYIL
                     AGTNYELDEFTNLVENSVYVISVDEGFAFKIGYGDPDDFITWWSPSENIVCWQDAQKE
                     PDKLKIYNIPVRKLSEIGLPYDGIIVKREEFSPERGLFSLLSAGIADLMVMDDGKVIV
                     QIKDNVYYINSETSEIKSVNGVFTSVYDGGIYFYNDNDIFKAEYDEFEPIRINSLEHS
                     IWKSKKAADNLHFYYGGAYNLSVFNTLDERIYWFTVDDYQGIIKPSPDGNRLYIYKDY
                     FSPYMPNNRVNGAFILDAGRKGEVVVETADDTAKWLDNDHMLINIYEETNKADKKHIL
                     SYVYEVETGERYMVKVVIDSFTSHVVNEFKMGFGTLTYEHKKPYSKLKEELKRNAYTK
                     SENTPIYTDSELLYTRGDILLKGSLVGVLEEGVETSKVCYFTEGNTLYSGWVRNTDFT
                     SNINLVKPAQGFIYNAKIYEFEKEEPKIVFENCSSQVRILDKKPGWVYINAVDRIFGW
                     IREEEIKFGFFADE"
     gene            3602..3733
                     /locus_tag="PK733_05965"
     CDS             3602..3733
                     /locus_tag="PK733_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10122.1"
                     /translation="MGEDRGDSPSVAGYEIFIVKIFSFAINMENLSILRKASRVIWQ"
     gene            3838..5070
                     /locus_tag="PK733_05970"
     CDS             3838..5070
                     /locus_tag="PK733_05970"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOJ10123.1"
                     /translation="MDIDLHQLNKISRKLLEYEKARITEIELRQYINVLDYEEYYNIV
                     VELAKNNIITPVKNSGSNGRRPPLYKRYNVIKPECSFDELIPEIRLLHDKLNIEGYLA
                     SPEKYKKHKPWLSLLDDFLKHNSKSLETPLSINERSFQIFKKEKALKEDKELVAVLNY
                     NPGLTEVLNYYHTPEPFFMYNITNNSIRGDIKGNNGNIRNNSNYNKYGNIIEPKEQHE
                     QYEQVGIEDIKQEPAAALQKLNREPDQKLNILIIENKDTWYTLKNIMSRNCNCLAGIH
                     FVSLIYGEGKKIARKMDSLTEFDKSFFDSVKTNYYYFGDLDYEGIGIFHDLINANPML
                     NIKLMKSLYTAMLDASKDIIEQLPVTKEKQAKKAEQLERFVSFFDSRYQAIIKNILEC
                     DRYIPQEILNNEDFKKLK"
     gene            5091..6716
                     /locus_tag="PK733_05975"
     CDS             5091..6716
                     /locus_tag="PK733_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10124.1"
                     /translation="MFDFLDNFKKRMEWMGIANSIINRKGKNTVLENLFEENELTNVI
                     VSVLLFIMEKTLEENNECEMYHIENFLDELLRIYFFKPFSRKQIKDIAGYIIKDILQN
                     NGVRYTYPVVNYENKKMDEITIRLIADKIIDENNERKIVYMLTNQGYEFLFRMREIDE
                     EIQLTIEQLKLKEYIKRKKFSSAVRQSVELISFVRQKKKDIENFILSIKQNIHNVDVE
                     QYEVLIKSTYAMLTEEYETMEDIQKMIHMAEEKIKEEFQMSRDFEQRLVKAKAEIMEI
                     HHNIGIVITEQRDLILNRYSLTDLYIDTIKKSFEFSFEKRYDFEETILLNLERYNNTL
                     DNCLCLFKPLFLPNKKKMLGIGDIYESQIIYKEVQNQDAHVVNIDEYDDSAEKERIRE
                     ISDRYVDIMALIITEAIKEDEICLSRILDDVRERNGEFYNRITEDRCIFLVALKLYDI
                     GRIPMEEFYQSGKRVLLRPSEEFNIEYCLVGIKDMIDKVDTIKELIVEKTGNEINISL
                     EKTEKVGKAEETEEKIKVIETIRMTDVIFKVVR"
     gene            6716..7444
                     /locus_tag="PK733_05980"
     CDS             6716..7444
                     /locus_tag="PK733_05980"
                     /inference="COORDINATES: protein motif:HMM:NF039975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6063 family protein"
                     /protein_id="HOJ10125.1"
                     /translation="MEIMEKMEITETMEHVRSAVKIFRLLITQGEINKREQAFLYSEY
                     LETEVQEVLSVFEEEFECKLLNFDDTVYLVPNINSQIIGVQPGELKRYFGSNATNKDV
                     YLGYYIMMFIFYEFYSGKNKDPKKTDFVQVSHLIDHLDERFERLQRLSKDEIEHLEEE
                     YSVNLASCIEIWMNLLVDHESRKKTKTKIIEGVVKILEENKLAYMVENQIRTTKKLDV
                     LMRQYYLNAERVGLISEAFEKGDL"
     gene            7445..11944
                     /locus_tag="PK733_05985"
     CDS             7445..11944
                     /locus_tag="PK733_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10126.1"
                     /translation="MPKINRVRIVNFSYNNNNRHIIDECFDFYGGENALLSLANGGGK
                     SVLVQAMLQPILPKVPLLGRKFRDFFSGKKTPSYIMIEWKLDDEAGYLLTGIAVAAST
                     SHSADEEDENTDIRYFTFLCSYEKGNEFDIKHIPVAEQVGSNVRIASYQDFKKLLKKE
                     SDRNQRNIFVFDSTREEQNQYDRKLGSYGISKEEWKELILNINEAEHGVSEVFSQCKT
                     SRKVMEQWVIKYIEKVLNKSADSEMTDHRKLEMMMAQVAQSLVDNEKHIREYRSIEAL
                     NRDLDSIYNDAEVVLTNLDNENKLKKDIGEGYCVLKDEEQRLERELSDMETRLRELDK
                     ELKTIELEEKSQEIYKYTEEIEVINGKIIDLGNSIEKEKLLLEEKKYKLNLQKAAEKY
                     DRKQEKEKSIAQLKQRLDNASKDQEALLKSLNQIKYSLKTAYNAEIANIRKGINDDSI
                     QLNNVRGNIDKNRIEARECQKQIEKLIKEIGSIESVIKGFEKEEPEILDALDVRVYRN
                     PLLKELDRQEIAKVKEQFLKEKQEVENLLNEKKNEIVKLKEALEDLQQRKEELSKKHT
                     GLKIEEAKIIDRIKSFQDEKQSVLDALKRLDINAEYLYDQVYLLKSAREYLNDWTNKE
                     YNIKMEISELERQIHGIEKGVSYLPASFLSLLQEQNLPCYTGEKYLREIDEETKKKLI
                     DNNPLLPYAIIATEKEIPLIEQLASDMELSQIIPIIPFSRRDQEIDLEQQDMRFISSS
                     RSINLSNEDIGNLVKDLIEKKERKILELKDAGEVIDRINHDCQIVGNFKWTQKKVEDL
                     ENELKVKKQEIETNDQAVKNCCQQIEDINNLQEKLTAQIEALKTDLPKKESRINTFEN
                     YLARDDAYMENLRLYNRIESKINTLAASKKKYEDENARLEEDAVELKEQLGSKNRSLK
                     DIEDKLKEVADARAVDFSVEDSSETDSSMVASPGMPDSSEGKVVDSSVFSIAELEGKL
                     EAYKKLQNNEVADLNEQIKGLIKDIQDIEKDIRKLKLATEEYKDISYSESAEMELEEL
                     CSKLAKEIKDLEDEKTKHDKKVAVLNDRIKTAKEALGDNPIVPIQEIKGSFSERRGEL
                     GRRRHENESANKKIQKSKLELAELLVEIKTEIKDIREIRADIDNKNRKSSEEIKAAIG
                     AMLMEYSSSVDDSNKSIDLFRNNSSRFINQYANYEEGTVKEAVKGLHSQIETLARTYE
                     KYYYLAERIEYYTEQLSQILKIMESKMQQLEHSKEDLTEHAFMEARRIYHEIPKISEN
                     SAVEIDGVRKRVLEIQYEEITDELRAREKMDVYIQECLQSLTKLIKSNEDESKLRREI
                     EKYMSTKELLNIISNLENCRIRAYKVDLNEKNRRMLPWEDIIIKNSGGEKFIAYFSLL
                     VALISYSRKQIKGYETFSRKEESKVLIMDNPFGPITSGHLLKPMFDIAEKYNTQLICL
                     SDIKQGSVVNSFNLIYMIKIRQNMMQEDFLEIEPVLLKELKQDEKLENVYLYRKSDQI
                     SIFEW"
     gene            11978..12529
                     /locus_tag="PK733_05990"
     CDS             11978..12529
                     /locus_tag="PK733_05990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10127.1"
                     /translation="MKEVYETQRLLLKVLDRSFAELVLDYYLRNKFFLEEWEAIKSEE
                     FYTKQYQEEQLDKELSNIENKISFRLWAFKKEDANRIIGSVSFNNIVRGAFLSCHLGY
                     KLDKDEVNKGYMTEAIQKGIEIIFNEFGLHRIEANIMPKNKRSLRVVEKLGFYNEGLA
                     YKYLKINGKWEDHIHMVLLNDKV"
     gene            complement(12584..13129)
                     /locus_tag="PK733_05995"
     CDS             complement(12584..13129)
                     /locus_tag="PK733_05995"
                     /inference="COORDINATES: protein motif:HMM:NF039668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HOJ10128.1"
                     /translation="MSLVLMDKEKQLQNVNVLPENVYHSYLGEYFLGQTDIISFGGGY
                     NAWGALVNPAGSKVNMFLNAYTITNFSNGPLTAEGWLSAILPGRRKTSPYFAAGNQAI
                     IPSPMPKVEIQNASFVSGNPRGGTYTFVRRVEPSETLTKHDFQGMYIIPPGSSFSLFF
                     LSPGNLQVYARVAFGWWEEKA"
     gene            complement(13166..13702)
                     /locus_tag="PK733_06000"
     CDS             complement(13166..13702)
                     /locus_tag="PK733_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HOJ10129.1"
                     /translation="MPKEMSKQLIEVVNITSPALNSRLGRYFIGQTGLLNFGNGYNAW
                     GGIINPIDSGVNLYFDIFTITNFSTQSFMGEIWLNASSPKNAVISSTVTPSNQGISPP
                     PIPKAVMKYADYFTEPMTGGVNIFGRIVAPNSTLVSDSHNGSIIIGPGGSFSILLRPL
                     VSQYIKGTIVLTWWEEAL"
     gene            14134..14691
                     /locus_tag="PK733_06005"
     CDS             14134..14691
                     /locus_tag="PK733_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ10130.1"
                     /translation="MEKDLRKRIKEVAVEHFNSNGYHGTTIRNIAKDVNCSLPMIYYY
                     YNNKKELFDEIIKKEYIDLLKRQAALLKINNIIDFYTKFVYDLNSLSNYDKKVYRLGI
                     KVYLSFDGDEELMEIMDEWEKTILPRHYQILKPYLRNVDNDVAIVRTLVHLLETLIEN
                     IVVKNRFIPEEEIREEISIVLRGCE"
     gene            14833..15318
                     /locus_tag="PK733_06010"
     CDS             14833..15318
                     /locus_tag="PK733_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10131.1"
                     /translation="MDMEKRFALLQNTYAASIAETVNTYDKLKVLDTIVERRKERQAQ
                     TAPFLNQQLGIETVEDVFCKLSEIYGCANWFVEKKDDGYIATAVSCKLCALSKKMGGA
                     NPCNGWCLDPMFAMITATGKIDAGSIAVESTLMTGDCCKVLVNVCTQQNDTAETALTY
                     G"
     gene            15336..16238
                     /locus_tag="PK733_06015"
     CDS             15336..16238
                     /locus_tag="PK733_06015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ10132.1"
                     /translation="MHLYYYENKNFNKIIDDGIRDFCLCERVNEEYMRSLNEQTHKVG
                     AHGAGGMMKMERKCTDQKKILLYGIMILLLLVFQKVLGKVGGNVADSLPCEKFDPYKA
                     FAWVSIHHITEMLIALAVIMILSKLLKVDFGLGLGDRKKGTKYVLVYTAIFAGVTLVC
                     HILMLIYNMLPVYNFPLNKNNILGTLGFQLFLSGPAEEILYRALPITMLMHLMGKSVK
                     VKWGITLETIIASFLFTIAHMNWTLFPFTIEANYFQLFYAFVLGIIQGKAYQDSRSIL
                     YPIFMHSISNVLMVGTGYLFIIFK"
     gene            16363..17832
                     /locus_tag="PK733_06020"
     CDS             16363..17832
                     /locus_tag="PK733_06020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOJ10133.1"
                     /translation="MNKNELFMPAPSPESLGIPSSAILNFIERIDREQTCMHGFLLLR
                     KGQIAAEGYWAPYSENSMHRMYSVSKSFVSLAVGLMIDEGKLNLDDKVVQFFKDKAPQ
                     DIHPYLAQSTVRDLLMMATPHTTNSYTRYDSDWAATFFNKKPSHPPGTIFSYDTAATV
                     VLNTIVERISGVPFLEYMRDKLLDPIGFSRDAWCIKTPEGTSWGGSGVICKLRDMAKL
                     AYVCMNKGRWNDLQLISEEYISAATSKQIDNSISGNDGYGYQIWRERDNGFAFRGMGS
                     QLAFCFPDKDFLFACISDTQGAGSTGTGITGAFHEEIFGKIKDFALPCEAESNAALKE
                     KIKNLKILPQRGNLSSSFEQGINNKWYTLNENPMGITRMRLIFNGDEGIWEYENASGH
                     HRLGFGIGKIIAGAFPQKNYYGERIGIIPGRCYDCLSSAAWVEEHKLNMLVYITDIYL
                     GTLKMSFSFKGNEISIYMTKVAEWFLDEYMGFAGGFFAE"
     gene            18463..18633
                     /locus_tag="PK733_06025"
     CDS             18463..18633
                     /locus_tag="PK733_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10134.1"
                     /translation="MVDRPAFDGVTSGAELISRYAGLFAVSYSFLKNAHTTTIEKYVK
                     IGFSKVSTTTEK"
     gene            18649..20199
                     /locus_tag="PK733_06030"
     CDS             18649..20199
                     /locus_tag="PK733_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10135.1"
                     /translation="MQGKGIGYSIKGVVYIVLVALISSILPVVSFAAEYTPPDVYSAP
                     QNVGVVFNDDISDSGRWSFDIGYAASGEVRKLMEAVADGRFEGASYDSLGIAAQIDYK
                     LDSGKWRSEMSGYEEWVMNSETGFDAAAGNWAASYHIYDGYFTDIFPDEVLPGGKSYF
                     DQHTMNFRIRFYITFHNSEKEKDYEYYSPWSSVVSYNNNQKVEDPAALINHPPKLLSV
                     ELKKADNGEPYLDFKADTAHADVQILNSISNQRVFTNVWIKVNNGEWIDAGTYLWMKE
                     QFTIEAKDYFGDVKDYSAAIYEAKFRYSFDYTYYPKAGKSGDIYSPFSNALAKGMPAY
                     KGASNWAITELNRAAEYGFITDKIKDNMKAPITREEFAEVAIKLYEAYTKKTAVPADS
                     SVFVDTKNPEIFKAYALKIVNGTNTEKKLFSPTLLTTRQEVSTMLYRAVKAINPDADL
                     STAGAPVFTDQEEIGSWAFEPVKYMSKQGFIKGTGAGAFDPKGTCTREMAVAIAVRVY
                     EKSGGQAR"
     gene            20385..20936
                     /locus_tag="PK733_06035"
     CDS             20385..20936
                     /locus_tag="PK733_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ10136.1"
                     /translation="MINEDRHIIEKILKGEQNEFSLIMEKYGQMVYRVCFGFMKNEQE
                     AMDMAQEVFISVYNNLDKFNFKSTLSTWIYRICTNTCLTQLNKLGKIQVCTEYIEPRD
                     YSCLLPDEVSELKELKELINIQMEKLPDKSKNIVKLRLFNQLSFNEIGEKLNIPSSSA
                     RTNFIRSRKRLQKAVNNYSKECE"
     gene            20940..>22096
                     /locus_tag="PK733_06040"
     CDS             20940..>22096
                     /locus_tag="PK733_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HOJ10137.1"
                     /translation="MMCNDITGITIRRLQGELNIKEKKKFDKHIKKCIECRRKYYFIT
                     ANYDPDAGYNQGYNQGYNQNKLIYNVMPRIDASRYDVSANRNGASVSANIKIKIKWAF
                     VCLAVIVSLSLTAFAKPIYKSLAELLETISINHNYYLKQAAGDVEKKVPEKKTEAEIK
                     NRQQSFAEADILYDKYKYKVDEIITEMKPGEVRTLTFEGERDDFYVSLGKGVEAETII
                     YGIKGISGLMQVEGIPTAYRARADNKDISIQEMIDQGCFTPVLEYLPEGYEFSGVGFS
                     KDEISSITYRNNKNNENDYISIYLATDRVNMDDDAKKTLEIINGYQAIYMEEEAIGNR
                     FGKFVIYLGENKRLKVLTLTANLKEGSIEKSDLYNIVENIKYYNQESNEIT"
BASE COUNT         7856 a         3257 c         4683 g         6300 t
ORIGIN      
        1 gcgttttatc aacataatat gcatttctat ctattaaatt tttataatct gatataccta
       61 taggcattcc cttttttctt gtttctccca taccaatacc acctttttgc atgaataatc
      121 ttatctataa cctattatat cacttttgtt aataacttac tatttaaaat ctaattaaaa
      181 aagcagatta aagaatcagt tacaggtcaa attcttccta tgatataaaa tatggcagca
      241 aacgattttc caagtataaa atttgaacaa agcttatgga ttgttatgtt taaggcaagg
      301 gaaaattcgt aattcacgac atggatgtcg tgagtcgtca cctgaatcat ggaggatgaa
      361 tgtaatgggt agaatttttc tgtaggctta aacgctacaa tccgttgctg aagtgaatat
      421 tatacatgga aatcgcctct taccatattt tccctcaaat gtagaaaatg cccccttcag
      481 agggtaagtt gcaattatat ggaaaatgtt atatactaga tatacacatt atttgaattg
      541 tgattttaat tttgttcact tttgtaaaga ctacccataa ttctttcaag ggaggggttc
      601 gttttgaaaa agactcttgt atgtcttgta ttcctagtat tggttttgtt tatttatccc
      661 agtatagccc tatctaattc acccccactt attatgaaag gagatacggc ttttcctata
      721 agcagtggaa gtataaagct agaaagtgaa gttattaaaa ttcattatgg aattacggaa
      781 aagcatgggg cactagggca tgaaattgag gtaattttta atttccataa cacaagctct
      841 gaaacagacc ttgatatagg cttcccaaat gttgccagtt atgcacaaaa actcagggac
      901 tttgaggctt atgactaccc atccatgact ccttatgaaa ctgaaattaa ggagggaggc
      961 actctgcccg agcatgatct ttatgattat aattccatgt atacatggaa aatgcatttt
     1021 gaagaaaatg aaaaaaaatc ggtgtatgta aaatataggt ttgaatcaca gcagtggggt
     1081 gcggattaca tacttgttac cggtgctctg tggaaggatc ggatagataa aatagatgta
     1141 tatgtggatt ttcctgaacc tgcagccttt gcacagatta ccgcttcccc tttaaactat
     1201 tactataatg gtaagggaat tgagtggcac tttgaaaaca ttgagcctga tttcaacctg
     1261 tttatcggct ttgtagattt acctgagttt tatgaaaaaa aagatgaata cttcaggcca
     1321 aggtggaatg atccgatgga tacttaccgg tgggatgatt gtttttacta tattgatctt
     1381 ttttcctcgt atgaggatat gggaaggatt gcactagtat ataataaaga tattgactat
     1441 attagaagag tagtcgaaag acttaaagac accaaccagt ttgttataaa tgaaatatat
     1501 gcaagacatg gttatgactt taaaaccaag aagtgggaaa agattttcga ggataccggc
     1561 tggtataaac atgaccccgg atttactcct gatgtgttta acagcatgga gcatgccact
     1621 attgcatata ttaattgttt taacaaaaca gttattactg atggaagtga tagcgaactg
     1681 atagattcca caaatgagtt ccaagataaa tataacggaa aagacatttt ttacagaggg
     1741 atgacatacc cctattacca atatacgtat aaccttgtat cctccgaaaa agaacggatt
     1801 aaacgtattg atgaaaataa tggacctttt ctcagtttgg gggggcagcc caaaggttta
     1861 agcgagcagc tggatggtga aatcaccggt gattatccgg cttccgaaaa gccgattaaa
     1921 actcttgatg tttccgatac aagaaaaatt gagctttatg aaaatggaat ttttgtaagg
     1981 gatggcaaag attcaaaatg tattttccgg tatcaaactt ttctaaaaac agaagacctt
     2041 ggaaagcata actatacgga ccctattgag tcagtcccct ggattaacga tgatataagt
     2101 gccagcccta tgggaaatta catcctggca ggcacaaatt atgagctgga tgaattcaca
     2161 aatttagttg aaaacagtgt gtatgttatt tcggttgacg aaggatttgc tttcaaaata
     2221 ggctatggcg accccgatga ttttattacc tggtggtcac cttctgaaaa tatagtttgt
     2281 tggcaggacg cccaaaaaga accggataag ctaaaaattt ataacatacc tgtaaggaaa
     2341 ctttcggaga ttggcctgcc ttatgatgga attatagtaa aaagagaaga attttcaccg
     2401 gaacgtggat tgtttagctt gcttagtgcc ggaattgctg atcttatggt aatggatgat
     2461 ggaaaagtca ttgttcagat aaaggataat gtatactata ttaacagcga aacaagtgaa
     2521 attaaaagtg ttaatggggt atttacctca gtttatgatg gtgggattta tttctataat
     2581 gacaatgata tatttaaggc tgagtatgac gaatttgagc caattaggat taacagtctt
     2641 gaacattcca tatggaaaag caaaaaagca gcagataatt tgcattttta ttacggaggt
     2701 gcttataatc tttctgtatt taatacgtta gatgaaagaa tatactggtt tacagttgat
     2761 gattatcagg ggattattaa gccttcacca gacggcaaca gactatatat atacaaggac
     2821 tatttcagcc cctatatgcc gaataatcgt gtaaatggag cttttattct ggatgcaggg
     2881 cgtaaaggcg aggtagttgt cgagacggct gatgatactg caaaatggct tgataatgat
     2941 catatgttga ttaatatata tgaggaaaca aacaaggctg ataaaaaaca catcttgtcg
     3001 tatgtttatg aagtggagac cggagaaagg tatatggtaa aagtagttat tgattctttt
     3061 acaagccacg tagtaaatga atttaaaatg ggttttggta ctctgacgta tgaacacaaa
     3121 aaaccttatt caaaactaaa ggaagaactt aaaaggaatg catatacaaa gtctgaaaac
     3181 acaccaattt acactgacag cgaactttta tatacacgag gtgatatact tttgaagggt
     3241 tcactggtcg gggtactgga agagggcgta gagacatcaa aagtatgtta ttttacagaa
     3301 ggaaatactt tatattcagg ctgggttagg aataccgatt ttacatccaa tataaatctg
     3361 gtcaaacctg ctcagggctt tatatataat gcaaaaattt atgaatttga aaaagaagag
     3421 ccaaaaatag tttttgagaa ttgctcaagc caggttagga tacttgacaa aaaacctgga
     3481 tgggtatata tcaatgctgt tgacaggata tttggatgga ttagggagga agagatcaag
     3541 tttggatttt ttgctgatga atgacatagg gtgacggagt ttatgtgtta tgggggaaga
     3601 catgggggaa gacaggggtg acagtccctc agttgccgga tatgaaattt ttatagtaaa
     3661 aattttcagt tttgccatca acatggagaa cctgtctata ttacgaaaag ccagtagagt
     3721 gatttggcag taatgagtat aggaacctat gaatggctcg ctgggagatt tgaattttat
     3781 gatatgatga tagaagaagg cacggatgcc aaaaaaatag aaatgggaag gaattaagtg
     3841 gatatagatt tacaccagtt aaataaaata agtagaaaac tacttgaata tgagaaagca
     3901 agaataactg agatagagct aagacagtat ataaatgttt tggattatga agaatattat
     3961 aatattgttg ttgaacttgc aaaaaacaac ataattacac ctgttaagaa ttcaggttca
     4021 aatgggcggc ggccgcccct ttataaaaga tataatgtaa taaagccgga atgcagcttt
     4081 gatgagctaa ttccggaaat ccgcttattg catgataagc tgaacattga aggttatctt
     4141 gccagccctg aaaaatataa gaaacataag ccctggcttt cattattgga tgatttcctg
     4201 aagcacaatt ccaaaagcct tgaaacacct ttatctatca atgaaagatc atttcaaata
     4261 tttaagaagg aaaaagctct aaaagaagat aaagagcttg ttgctgtcct taattataat
     4321 ccggggttaa cggaagtttt aaattattat catacaccgg agcccttttt tatgtataat
     4381 attactaata atagtattag aggtgacata aaaggcaata atggaaacat aaggaataat
     4441 agtaattata ataaatatgg taatattatt gagccaaagg aacaacatga gcaatatgaa
     4501 caagtcggga tagaggatat aaaacaagag cctgctgctg cgttacagaa gcttaatcga
     4561 gaacctgatc aaaagctaaa tatactaatt attgagaaca aggatacctg gtatacttta
     4621 aaaaatataa tgtcgcgtaa ttgcaactgc ttagccggaa tacattttgt cagcttaatt
     4681 tacggagaag gcaagaaaat tgctagaaaa atggattcct taactgaatt tgataagagt
     4741 ttttttgata gtgtaaaaac aaattactac tattttggag accttgatta tgaaggaatt
     4801 ggaatattcc atgatttgat taatgccaat cccatgctga atattaagtt gatgaaatcc
     4861 ctttatacag ccatgctgga tgcttcaaaa gacataatcg aacagctgcc ggttaccaag
     4921 gaaaaacagg ctaaaaaagc cgagcaattg gaaaggtttg tttcattttt tgattccagg
     4981 tatcaagcaa ttatcaaaaa catattggaa tgtgataggt atatacctca ggaaatactt
     5041 aacaacgagg attttaaaaa attaaaataa ggatggtgaa aggaaaggga atgtttgatt
     5101 tcctggataa ttttaaaaaa aggatggagt ggatgggtat tgcaaactcc attattaata
     5161 gaaaagggaa aaatacagta cttgagaatc tatttgaaga gaacgaactg accaatgtca
     5221 tagtatcggt tcttttattt attatggaaa aaactcttga ggaaaataat gaatgtgaga
     5281 tgtatcatat tgagaatttt cttgatgaat tattgaggat ttactttttc aaacctttta
     5341 gcagaaaaca gattaaagat attgcaggat acatcattaa ggacattctg caaaacaatg
     5401 gtgtgagata tacttacccc gttgttaatt acgaaaacaa aaaaatggat gaaatcacca
     5461 tcaggctcat tgctgataaa ataattgatg aaaacaatga acgtaagatt gtatatatgc
     5521 tcaccaatca gggatatgaa tttttattca gaatgaggga aatagacgaa gaaatccagt
     5581 tgaccataga gcagctgaaa ttaaaagagt acataaaaag aaagaaattt tccagcgcag
     5641 taaggcaaag tgttgagctt ataagttttg tacgccaaaa gaagaaagat atagaaaact
     5701 tcatattgtc tatcaagcaa aatattcata acgtagatgt tgaacagtat gaagtattaa
     5761 taaagagtac atatgccatg ctgacggaag aatacgaaac tatggaagat atacagaaga
     5821 tgatacacat ggctgaagaa aaaatcaaag aagaatttca aatgagcagg gacttcgaac
     5881 aaagacttgt caaagcaaaa gcagagatta tggagataca ccataacata ggtattgtta
     5941 taaccgagca aagggattta attctcaaca gatacagcct gactgatttg tacattgata
     6001 ctataaaaaa atcctttgaa ttcagttttg aaaagaggta tgattttgag gaaactatac
     6061 tgttaaacct tgaacggtac aataatactt tggacaattg tttgtgtttg tttaaacccc
     6121 tgtttctgcc taacaagaag aaaatgttgg gtattggtga tatttacgaa tctcagatta
     6181 tttacaaaga ggtgcagaac caggatgctc acgtggttaa tattgatgaa tatgacgatt
     6241 ctgctgaaaa agagagaatt agggaaataa gcgacaggta tgttgatatt atggctctta
     6301 taattacaga ggcaataaag gaagatgaaa tttgcctgag ccggattttg gatgatgtca
     6361 gggagaggaa tggagaattt tacaacagga ttacagaaga tagatgtatt tttttagttg
     6421 ccctcaaact atatgatatt ggcaggatac ccatggagga attttatcaa agcgggaagc
     6481 gtgtgctgct tcggccttcg gaggagttta atattgagta ttgtcttgtt ggaattaaag
     6541 acatgataga taaagttgat actataaaag agttaatagt ggaaaagaca ggtaatgaaa
     6601 ttaatatatc attagagaaa acagagaagg taggaaaagc ggaggaaaca gaggaaaaaa
     6661 taaaagtaat tgaaactatc cggatgacag atgtgatttt caaggtggtg agataatgga
     6721 aataatggag aaaatggaga taacagagac aatggaacat gtaagatcag cggtgaaaat
     6781 attcaggctg ctgattactc aaggagaaat aaacaagagg gaacaggcct ttttatatag
     6841 tgaatatctt gaaactgaag tacaggaagt gctgtcagtt tttgaagagg aattcgagtg
     6901 caagcttctg aattttgatg acacagttta tctggtgcca aatatcaaca gtcagattat
     6961 tggtgttcaa ccgggtgaac tgaaacgcta tttcggcagc aacgcaacaa ataaagatgt
     7021 gtatctagga tattatataa tgatgttcat tttttacgaa ttctacagcg gtaaaaataa
     7081 ggatcccaag aagacggatt ttgtccaggt aagtcattta attgaccacc tggatgaaag
     7141 atttgagaga cttcaaagat taagtaaaga tgaaattgag catcttgagg aagaatactc
     7201 agttaatttg gcttcatgca ttgagatttg gatgaatctc ctggtagacc atgagtccag
     7261 gaaaaaaaca aagacaaaaa taattgaggg cgttgtaaaa atattggaag aaaataagct
     7321 ggcatacatg gtggaaaatc aaatacgtac tacaaagaag ctggatgtgt taatgcggca
     7381 gtattatctg aatgcagaaa gggtagggct tatcagtgaa gcttttgaaa agggggattt
     7441 gtaggtgcct aaaataaaca gagtcagaat agttaacttt tcatacaaca ataataaccg
     7501 acatatcata gatgagtgtt ttgattttta cggcggagaa aatgctttgt taagccttgc
     7561 taatggagga ggaaaatcag tccttgttca ggccatgctg cagcccattt tgcccaaagt
     7621 gccattattg ggcagaaaat tcagagattt ttttagtggt aaaaaaaccc cttcctatat
     7681 catgattgaa tggaagctgg atgatgaagc aggctatttg cttacaggca ttgcggtagc
     7741 tgccagtaca agccattctg cagatgagga agatgaaaat acagatatca gatattttac
     7801 atttttatgt tcatatgaaa aaggcaatga atttgatatt aagcacattc ccgttgccga
     7861 acaggtcggc agtaatgtga ggattgcttc gtatcaggat tttaaaaaac tcctgaaaaa
     7921 ggaatcagat agaaatcaaa ggaatatatt tgtttttgac tctacccggg aagaacagaa
     7981 tcagtatgac aggaaactag gaagctacgg tatttcaaag gaagaatgga aagagctcat
     8041 tttaaatata aatgaagcag aacatggtgt gtccgaggtt ttttctcaat gcaagacttc
     8101 caggaaagtt atggaacagt gggtgataaa atacattgaa aaagtattga acaaatcagc
     8161 tgattctgaa atgacagatc ataggaagct ggaaatgatg atggctcagg tagcacaatc
     8221 actggttgac aatgaaaagc atatcaggga gtaccggtca atagaagctc ttaacaggga
     8281 tcttgacagt atatataatg atgctgaagt cgtactgacc aatcttgata atgaaaacaa
     8341 gctgaaaaag gacatcggtg aaggatactg tgttttaaag gatgaggagc aaagactgga
     8401 acgggaactt tcagatatgg aaacccgcct aagagagctt gataaggagc ttaaaacaat
     8461 tgaacttgag gagaaatcac aggaaatata taaatatact gaagaaattg aagtaattaa
     8521 tggcaagatt atagatttag gtaatagtat tgaaaaggaa aaactacttc ttgaagagaa
     8581 aaagtacaag ttgaatttgc aaaaagctgc tgaaaaatac gataggaaac aagagaagga
     8641 aaaatccatt gcccagctta agcagcgact tgataatgca tcaaaggatc aagaggctct
     8701 gctgaaaagt ctgaatcaga taaaatattc tttaaaaaca gcttataacg ctgagattgc
     8761 aaacatcagg aaaggaatta atgatgatag tatccaatta aataatgttc ggggaaatat
     8821 tgataaaaac agaattgaag caagagaatg ccagaagcaa atagaaaaac ttattaagga
     8881 aattggcagt atcgagtcgg taattaaagg ttttgaaaaa gaagaaccag aaatacttga
     8941 tgctctggat gttcgagtat accgcaatcc tttactgaag gaactggatc ggcaggaaat
     9001 tgcaaaagtg aaagagcaat ttctaaaaga gaagcaggaa gtggaaaatc tcctgaacga
     9061 aaagaagaat gaaattgtca agcttaaaga ggcacttgag gatctgcagc aaaggaaaga
     9121 agaactctct aaaaagcata cagggcttaa aattgaagaa gcaaaaatta ttgacagaat
     9181 caaaagcttc caggatgaaa agcagtctgt tcttgatgct ctaaagaggc tggatattaa
     9241 tgctgaatat ttgtatgatc aggtatatct tttgaaatct gccagggaat atctgaatga
     9301 ctggacaaat aaagaataca acataaaaat ggaaataagt gagctggaaa gacagataca
     9361 tggcattgaa aaaggcgtca gctatctgcc cgcaagtttc ttatccctat tgcaggagca
     9421 gaatctcccc tgctatacag gtgagaaata tttgagagag attgatgagg aaacaaagaa
     9481 aaagttaata gacaacaacc ctttgctgcc ctacgccatt attgcaactg aaaaggaaat
     9541 tccattaatt gaacagcttg catctgatat ggaattaagc cagatcatac cgattatccc
     9601 ttttagccgg agggatcagg aaatagacct ggaacagcag gatatgcgtt ttatctcatc
     9661 atccaggagt ataaatctgt ccaatgagga cattggcaat ttagtcaagg atctgataga
     9721 aaagaaagag cgaaaaatac ttgagcttaa agatgctggt gaggttattg acagaataaa
     9781 tcatgactgc cagattgttg gaaattttaa atggactcaa aagaaagtag aagacctgga
     9841 gaatgagcta aaagtcaaga aacaggaaat agagacgaat gaccaggccg tgaaaaactg
     9901 ctgtcagcaa atagaggaca taaacaacct gcaggagaaa ttaacagccc aaatagaagc
     9961 tttaaaaact gatcttccta agaaagaaag caggataaat acttttgaaa attatctggc
    10021 cagagatgat gcatatatgg agaaccttcg cttatacaac cgaattgaaa gtaaaatcaa
    10081 cacattggct gcttcaaaga aaaaatatga agatgaaaat gccagattag aggaagatgc
    10141 agtagaatta aaagaacagc ttggcagtaa aaatagaagc ctgaaggata ttgaagataa
    10201 actgaaggaa gtggcggatg ccagagcggt ggatttcagt gtagaggatt ccagtgaaac
    10261 ggattcaagc atggttgctt ctcctggcat gccggattcc agtgaaggca aggtggttga
    10321 ttcgagcgtt tttagtattg ctgaacttga gggaaaactg gaagcatata aaaaactgca
    10381 aaataatgag gttgctgatt tgaatgaaca gattaaggga ttaataaagg atattcagga
    10441 tattgagaaa gatataagga aattaaagct ggcaacagag gaatataaag atatttccta
    10501 ttctgaaagt gctgaaatgg agctggaaga actgtgcagc aaactggcaa aagaaataaa
    10561 agatctggaa gatgaaaaaa caaagcatga taaaaaagtc gctgtgctaa atgatagaat
    10621 taaaacagcc aaggaagccc ttggagataa ccccatagtg cctatacaag aaataaaagg
    10681 aagtttctct gaaagacgtg gtgagcttgg caggaggaga catgaaaatg aatctgctaa
    10741 taaaaagata cagaaaagta aacttgaact agctgagctg cttgtcgaaa taaagacgga
    10801 gattaaggac ataagggaaa tacgggctga tatagataac aagaaccgga aatcttctga
    10861 ggaaataaaa gctgcaattg gtgctatgct gatggaatat agcagttctg tggatgattc
    10921 caacaaatcc atagatttgt tccgtaataa cagcagcaga ttcattaacc agtatgcaaa
    10981 ttatgaagag ggtacagtca aagaagcggt aaaaggtctc catagtcaaa ttgaaacatt
    11041 ggcacggacc tatgaaaaat attattatct ggctgaacgg atagaatatt ataccgaaca
    11101 gctctcacaa atccttaaaa taatggagag caaaatgcag cagcttgaac acagtaagga
    11161 ggacttaacc gagcacgcat tcatggaagc caggaggatt tatcatgaga taccaaaaat
    11221 ttccgaaaat tctgcagtag aaatagatgg cgtaaggaaa agagtcctgg aaattcaata
    11281 tgaagaaata acagatgaac ttcgggccag ggaaaaaatg gatgtatata ttcaggaatg
    11341 cttgcaaagt ttaacaaagc ttattaaaag caatgaagat gaaagtaaat taaggcgtga
    11401 aattgagaaa tacatgtcaa ccaaagagct tttaaatatt attagtaatc ttgaaaattg
    11461 caggatcagg gcttataagg tggatttgaa tgaaaaaaac cgcagaatgc tgccctggga
    11521 ggacatcata ataaaaaatt ccggcggcga aaaattcatt gcatattttt cactgcttgt
    11581 agcattgatt tcatatagcc gcaaacagat aaagggatat gaaacattca gtagaaagga
    11641 agaaagcaag gtcctgataa tggacaatcc attcgggcct attacctcag gacatcttct
    11701 aaagcccatg tttgatatag cagaaaaata taacactcag ttgatctgcc tgtccgatat
    11761 aaagcaggga tcggttgtca atagttttaa cctgatttat atgataaaga taagacaaaa
    11821 catgatgcag gaagatttcc tggagataga acctgtgctc cttaaggaac ttaaacagga
    11881 tgagaagctg gaaaatgtat atttgtatag gaagtctgat cagataagta tttttgaatg
    11941 gtaagtggga gcttttgaaa ttgatggggg ttattttatg aaagaagttt atgaaacaca
    12001 aagacttttg ttaaaagtat tagacaggtc cttcgccgaa ttggttctag attattattt
    12061 aagaaataag ttctttctag aagagtggga agcaataaag agtgaagaat tttatacaaa
    12121 gcagtatcag gaagaacaat tagacaagga gttatctaat attgaaaaca aaatttcctt
    12181 ccgattatgg gctttcaaaa aggaagatgc taatagaata ataggctctg ttagctttaa
    12241 caatattgta agaggagcgt ttctatcctg tcatcttggt tataaattag ataaggatga
    12301 agtaaataaa ggctatatga ccgaggcaat tcaaaaggga attgagatta tatttaatga
    12361 atttggacta catagaattg aggctaatat catgcccaaa aataaacgct ctttaagggt
    12421 agttgaaaag ttaggatttt ataacgaggg actagcatat aaatatttaa agataaatgg
    12481 gaaatgggaa gaccatatac atatggtttt gctaaacgat aaggtgtaat aaaggttatt
    12541 cattataaat taaggacaaa gacctaatgt cctttaattt tgatcatgct ttttcttccc
    12601 accagccaaa ggcgactctc gcatatacct gtaaattccc cggtgacaga aaaaataggg
    12661 aaaaagaact tcctggaggt atgatataca taccttgaaa atcatgcttt gtaagggttt
    12721 cactgggttc aactcttctt acaaaggtgt aggtaccacc tctagggttt ccagatacaa
    12781 agctggcatt ttgaatttca accttgggca ttggcgatgg aataattgcc tgattaccgg
    12841 cagcgaaata tggcgatgtt tttcttctcc cgggaagtat ggcgcttagc cagccttcag
    12901 ctgttaatgg cccatttgaa aagttggtaa tagtataggc attcaaaaac atattaacct
    12961 tggagccagc tgggttgact agtgcgcccc atgcattgta tcctccaccg aaagatataa
    13021 tatcagtttg acccagaaag tattctccaa gataggagtg atatacgttt tctggcagca
    13081 cgttgacatt ttgaagttgt ttttctttat ccattaatac taagctcata atcttcctcc
    13141 tcataatcat gcattgactg atgagtcata atgcttcctc ccaccaggtc aaaacgattg
    13201 tccccttgat gtattgagat accaacggcc ttagaagtat tgaaaaggag cctccggggc
    13261 cgataattat tgagccattg tgtgaatcgc ttacaagggt tgaatttggg gcaacaattc
    13321 taccaaaaat attcacacct ccagtcattg gttcagtaaa ataatcggca tacttcatta
    13381 cagcctttgg tattggagga ggtgaaatac cctgattcga tggagttaca gtggaggaga
    13441 ttactgcgtt tttaggcgaa ctggcattta gccagatttc ccccataaag ctttgagttg
    13501 aaaagtttgt tatggtaaaa atatcaaaat atagattcac tcctgaatca ataggattta
    13561 ttattccacc ccaggcatta tatccgttgc cgaaattcag aagcccggtt tggcctataa
    13621 aatatctacc aagccttgaa tttagggctg ggctggttat gtttactacc tcaattagtt
    13681 gtttactcat ttctttcggc atactattac tccggactgt gcattctcat aattatatta
    13741 tatgaaacag tataggaagt tgttcaattt gatattgaaa tagacttaat gaaataggac
    13801 atgcctttat aaagcaagtc cttgatttac attatgtata cctctgatta gaccggacag
    13861 cacattgcat ttttaagtgg aattttccac aggcttttag ttggaatctt aattctcatc
    13921 aaacaaattg cgtttaattt catcgcacag cgctgtaagt tggctaatag caaagttgct
    13981 ggtttgaaca ttttcacgta cgatgttgat tacatctttt aattattcaa ttagcctcaa
    14041 aaattttgta aaaatatttc tgaaaaccta ttgcattttt aaataatagt tgatataata
    14101 ctaacgaacg ttaggtaaag gagagtatat atattggaaa aagatctgag gaaaagaatt
    14161 aaagaagtag cagtagagca tttcaacagt aacggttacc atggaacaac gatccgcaat
    14221 atagcaaaag acgtgaactg ctccctgcca atgatatatt actattacaa taataaaaaa
    14281 gagttatttg atgaaatcat aaaaaaggaa tatattgacc ttttaaaaag gcaggcggca
    14341 ctcttgaaga taaataacat catagatttt tatacaaagt ttgtgtatga tctaaactct
    14401 ctgagcaact atgataaaaa agtttatcgg cttggaatca aggtttatct gtcctttgat
    14461 ggcgatgagg aattgatgga aatcatggat gagtgggaaa agactatact tccacgacat
    14521 tatcagatat taaaaccata tctgaggaac gtagacaacg atgttgcgat tgtacgaaca
    14581 ttagtacact tattggaaac attgatagaa aatattgtcg tcaaaaacag gtttatacct
    14641 gaagaggaaa tccgagaaga aatttcaatc gttcttcgtg ggtgtgaatg atttgttttc
    14701 ctcagttgaa gtaaaacttg caaatggatt taaacgttct tcgcgaatgt tagtgtttta
    14761 ttttcatgtt aatattttat tttccgcaat acctaacgaa cgttaaatta aatatcaaag
    14821 gagagttgga aaatggatat ggagaagagg tttgccctat tgcaaaatac ctatgcagca
    14881 tctatagcag agactgtgaa cacttatgat aagctgaagg tgcttgacac aattgtagag
    14941 agaaggaaag aaaggcaggc acagacagca ccctttttga atcaacagct tggtattgaa
    15001 actgttgagg atgttttttg caagctttca gaaatttatg gctgtgcaaa ctggtttgtg
    15061 gaaaaaaaag atgatggcta tatcgctacg gcagtttcgt gcaagttatg cgcactgtcc
    15121 aagaaaatgg gaggagcaaa tccttgcaat ggatggtgcc tagatcccat gttcgctatg
    15181 atcactgcca caggcaaaat cgatgctgga agcatagctg ttgaaagcac gttaatgacc
    15241 ggagactgct gtaaagtgct tgtcaatgtc tgtacacaac agaatgatac tgctgaaact
    15301 gcattgacat atggataatg aaagaaggct ggaaaatgca tttatattat tacgaaaata
    15361 agaacttcaa taaaatcata gatgacggca taagggattt ttgtttatgc gagagagtaa
    15421 atgaagaata tatgaggtca ttgaacgagc aaacccataa agttggagcc catggagctg
    15481 gaggcatgat gaaaatggaa agaaaatgta ccgatcagaa aaaaatactg ctatacggga
    15541 tcatgatttt gctgctgctt gtttttcaga aagtactagg taaagtgggg gggaacgtcg
    15601 ctgattcact cccctgtgag aagtttgacc cttataaagc ctttgcatgg gtttctattc
    15661 atcatatcac tgaaatgttg attgccctgg ctgtaattat gatactaagc aagttgctga
    15721 aagttgattt cggtttaggt cttggcgaca ggaaaaaagg aaccaaatat gtattggtat
    15781 atactgccat atttgcaggg gttacgcttg tttgccatat actcatgcta atctataaca
    15841 tgctgccggt atacaacttc ccattaaaca aaaacaatat acttggaaca ctgggctttc
    15901 aattgttctt aagcgggcct gccgaagaga ttctgtatcg ggcactcccc attacaatgc
    15961 tcatgcattt aatggggaag agtgtaaaag taaaatgggg aattacatta gaaacaatta
    16021 tcgcctcttt cttgtttaca atcgcccata tgaattggac tttattcccg tttaccattg
    16081 aagctaatta ctttcagctg ttctatgctt ttgtactagg tattatccag ggcaaagcat
    16141 accaggatag ccggagcata ttatatccga tattcatgca cagtatcagc aatgtgctga
    16201 tggttggaac aggttatctg tttataatat ttaaatagtg cttaaaggag gagtttcgaa
    16261 ggttttgtgc attagggtgc attaacattc aaaagtgtca atttgtaatt tccatgctat
    16321 aataaaaatg tatttaaaaa attaaaagga gcgtggttaa ctttgaataa aaacgagttg
    16381 tttatgccgg cgccttcacc cgaaagttta gggattcctt cttcagcaat tctcaatttt
    16441 attgagagaa tagacaggga gcaaacatgc atgcatgggt ttttattgct gagaaaaggg
    16501 caaatagcag ctgaaggcta ctgggcacca tattcagaga acagtatgca tcgcatgtat
    16561 tccgtaagca aaagctttgt atctttagct gtaggattaa tgattgatga aggaaaacta
    16621 aatctggatg ataaggttgt tcaatttttt aaggataaag cacctcaaga catacacccc
    16681 tatttggctc aatcaaccgt aagagatctt ttaatgatgg caacacccca tacaacaaat
    16741 tcttatacac gctacgatag tgactgggct gcaacatttt ttaataaaaa gccttcacat
    16801 cctccaggaa caatcttttc ctatgataca gcagctacag ttgttttgaa tactattgtg
    16861 gagcgaataa gcggagttcc tttcctggag tatatgcgtg ataaattact tgatcctatt
    16921 gggttttcca gggatgcctg gtgtataaaa acacctgaag gtacatcatg gggaggttca
    16981 ggagttatat gcaagttaag agatatggct aaattagctt atgtatgtat gaataaaggc
    17041 cgttggaatg atcttcaatt aatatcagaa gaatatatta gtgcagctac atcaaagcaa
    17101 atagacaact ctatttcagg taatgacgga tatggatatc agatatggag ggagagagat
    17161 aacggattcg ctttcagagg gatgggttca cagcttgcat tttgttttcc tgataaggat
    17221 tttctatttg cctgtatatc tgatacacaa ggtgcaggtt ctacgggaac aggaattact
    17281 ggagcttttc atgaagaaat attcggcaaa ataaaagatt ttgctttacc ttgcgaagcc
    17341 gagtcgaatg ctgcactaaa ggagaaaatc aaaaatctaa agatcctacc gcagcgagga
    17401 aatctatcat cttcattcga gcaaggaatc aataataaat ggtatacttt aaatgaaaat
    17461 ccaatgggaa taaccagaat gcgtttaatt tttaatggag acgaaggtat atgggaatat
    17521 gaaaatgcat cgggacatca caggcttgga tttggtatag gaaagataat tgcaggagct
    17581 tttcctcaaa agaattatta cggagaaaga atcggaataa tacctggcag atgctatgat
    17641 tgcctttcct cggcagcatg ggttgaagaa cataagctta acatgcttgt atacataact
    17701 gatatctacc ttggcaccct caaaatgtct ttttcattta agggaaatga gatatctatt
    17761 tatatgacta aagtagcaga atggtttttg gatgagtata tgggatttgc aggtggattt
    17821 tttgcagagt aaagctatat agaagcaggt tttaaaacat ttaaaacctg cttctgttac
    17881 cttgatgtaa cttacgattt gcaaattctt aattaggttc tttcaaatcg taaattatat
    17941 attgacacaa agggagattt atgaaaaaat tatttcagat agaatataaa taaggggcac
    18001 atttttaatt tttgaagaaa tgcattccag aagctattgt tatggtgctt cttccgataa
    18061 tttctccaaa aaataatatg cgtcgcccat aaataagggc aaatttgccc aagctatgta
    18121 tattttgaat ttataacagt atttttaatt atagattaga aaagaaagaa ggtgaatgaa
    18181 ttgtatgcaa cacgatctta atgaaaccta tcaccaacag cagtcccgtg aacctatacc
    18241 acaaaagcat gtacatacat ctataaaaag gactgcaaag aaaagacatt tcatgaggaa
    18301 aaaaagtttg tttctttttc aaaaaaagtt ggcaatgctt attacagacg tctaaaagct
    18361 gatcaaaaag cctggtatga tgagcaggca ggtaaaattg gggagaatgt attcattata
    18421 ccagataagc ctatcctttt ggcatcggct ggtgttgaca ttgtggttga taggcccgca
    18481 ttcgatggag taacttccgg agctgagctg atatctcgat atgcaggttt atttgcagta
    18541 tcgtactctt tcttgaaaaa tgcgcacaca acaactattg aaaaatacgt taaaataggt
    18601 tttagtaaag tatccacaac aactgaaaaa taacaaggag gcatttatat gcaaggaaaa
    18661 ggaataggat atagtattaa aggagtagta tacattgtat tagtggcatt aattagcagt
    18721 attttaccgg tggtttcatt tgctgcggaa tatactccgc ctgacgtcta tagtgcaccg
    18781 caaaatgtag gagttgtgtt taatgatgac ataagtgatt ctggaagatg gagttttgat
    18841 attggctatg cggcatccgg cgaagtgcga aagcttatgg aagcagtggc agatggccgt
    18901 tttgaaggag catcatatga tagccttggc atcgctgcac agatagatta taagctggac
    18961 agcggcaaat ggaggtctga aatgtccgga tatgaggagt gggtgatgaa ttctgaaaca
    19021 ggttttgatg ctgcagcagg aaattgggca gcttcatacc acatatatga tggctatttt
    19081 acagatattt tccctgatga agttttaccg gggggcaaat catattttga tcagcataca
    19141 atgaacttcc gcattagatt ttatataact ttccataact ctgaaaaaga gaaggattat
    19201 gaatattatt caccctggtc ctcggttgta tcctataaca acaatcaaaa agtagaagac
    19261 cctgcagctt taattaatca tcccccaaag cttttatcag ttgagctaaa aaaagctgat
    19321 aatggggaac cctaccttga tttcaaagca gacacggcac atgcggatgt acagattcta
    19381 aacagcatat ccaatcagag agtatttaca aatgtttgga ttaaagttaa taatggcgaa
    19441 tggatagacg caggaactta tttatggatg aaagagcaat ttactatcga ggcaaaagat
    19501 tattttggtg atgttaaaga ttattctgct gcaatctatg aagcaaaatt caggtattcc
    19561 tttgattata cgtattatcc caaggcagga aagtcagggg atatttattc tcctttttca
    19621 aatgccttgg caaaaggtat gcctgcctat aaaggagctt caaactgggc aattacggaa
    19681 cttaacaggg cagctgaata tgggtttatt accgacaaga taaaagataa catgaaagcg
    19741 ccaattacaa gagaggaatt tgctgaagtt gcaattaaac tatatgaagc atacacaaaa
    19801 aagactgctg ttccagcaga ctcaagtgtg tttgtagaca caaaaaatcc tgaaatattt
    19861 aaagcttatg ctttaaaaat tgtaaatgga acaaatacag aaaagaagct tttttctcca
    19921 accctgctga ccaccaggca ggaagtatcg actatgttat atcgtgcagt aaaagctata
    19981 aaccctgatg ctgatttatc tacagcaggg gcacctgtat ttaccgacca ggaggaaatt
    20041 ggaagctggg cctttgaacc ggtaaaatat atgagcaaac agggttttat taaaggtaca
    20101 ggagcgggtg catttgatcc taaaggaact tgcactaggg aaatggcagt agctatagca
    20161 gtcagagtgt acgaaaaatc agggggacag gctcgttgat tgctcgttga ttggctcgtt
    20221 ggttgactcg tcggtttgac ttcatttgat ggtcgggggt gtgtaaaaca gaaatgttta
    20281 accgcctcct ttttaatata taatgtaaat aaattcacac aacttgtagc ttttttcatt
    20341 tattcaagtc taatatgttg aagaggtcct ctggaggtga actcatgata aatgaagata
    20401 ggcatattat tgaaaaaata ttaaaaggtg aacagaatga attttccttg atcatggaga
    20461 aatatgggca aatggtttat cgtgtatgtt ttggatttat gaaaaatgaa caggaagcta
    20521 tggatatggc tcaagaggta tttatatcag tatataacaa tttggataag tttaatttta
    20581 aaagtacatt atctacatgg atttacagaa tttgtacaaa tacctgcctt actcagttga
    20641 ataagcttgg taaaatacag gtttgtactg aatatattga gcccagagat tatagctgcc
    20701 tattacctga tgaagtttct gaacttaaag agttaaaaga attaattaat attcaaatgg
    20761 aaaaattacc tgataaatct aaaaacattg taaagttgag gcttttcaac caattaagtt
    20821 ttaatgaaat tggagaaaaa ctcaatatac caagttcatc agctcgtaca aatttcataa
    20881 gatcaaggaa acgactgcaa aaagcagtaa ataattatag caaggagtgt gaataggtaa
    20941 tgatgtgtaa tgatattact ggtattacta ttagaagact tcagggtgag ttgaatataa
    21001 aagagaaaaa gaaatttgat aaacatatta aaaaatgtat tgagtgcaga agaaaatatt
    21061 atttcataac agctaattat gaccctgatg caggatataa ccaaggatat aatcaagggt
    21121 ataatcagaa taaattgatt tataatgtca tgcccagaat agatgcttcc agatacgatg
    21181 tatcagcaaa caggaatgga gcatcggttt ctgcaaatat taaaatcaaa ataaagtggg
    21241 cttttgtatg tcttgctgtt attgtttcat taagtttaac cgcttttgcc aagccaatat
    21301 ataaaagctt agcagaatta ctggaaacaa tttcaattaa tcataattac tacttaaagc
    21361 aggctgccgg tgatgtagag aaaaaagtcc ctgagaaaaa gactgaggct gaaattaaaa
    21421 ataggcaaca aagctttgca gaagcagata ttctctatga caagtataag tataaagtag
    21481 atgagattat tactgaaatg aaacccggag aggtaagaac attaaccttc gaaggggagc
    21541 gtgatgattt ctatgtaagc ttaggaaagg gtgtagaagc tgaaacaata atatatggta
    21601 ttaaaggaat cagtgggctt atgcaagtag agggtattcc aacagcatac agggcgaggg
    21661 ccgacaataa agacatatca attcaagaaa tgatagatca aggctgtttt acacctgttc
    21721 ttgaatacct gcctgaaggt tatgagtttt ctggtgttgg tttttccaaa gatgaaataa
    21781 gttctataac atataggaat aataagaata atgaaaatga ctatatttct atatatcttg
    21841 ctactgatag agttaatatg gatgatgacg cgaaaaaaac acttgaaata attaatggct
    21901 atcaggcaat ttatatggag gaagaggcta taggcaaccg gtttgggaaa tttgtgattt
    21961 accttggtga gaacaaacgt ctaaaagtct taactctcac agccaattta aaagagggtt
    22021 ctattgaaaa gtccgatctt tataatattg tagagaatat aaaatattat aatcaggaaa
    22081 gtaatgaaat aactta
//
LOCUS       DAOSDD010000046        21982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1030_length_21982_cov_5.196926, whole genome shotgun sequence.
ACCESSION   DAOSDD010000046 DAOSDD010000000
VERSION     DAOSDD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21982
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1030_length_21982_cov_5.196926"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..2550
                     /locus_tag="PK733_06045"
     CDS             79..2550
                     /locus_tag="PK733_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10138.1"
                     /translation="MKRFIALGLSIILLCAAMNVFAAAGTVGSVVGADTYDKASYYNT
                     FKELKVLGILKYCDTSLLAPGNLLTRKQFAGILTKLAGLDEYPDLLIGAPDFKDVDPK
                     DPSGKCIKAVYKKDLITSMPDGMFHPDEPVTFAQFCTAAVKLLGYGDGDIIGLWPKNY
                     IDKAQSLEITKGLNFRHGDELPIWTAFHIIQILFDTEIKKTASDQVSKTYAEKMGFYE
                     EFLVLADSNILQTLDKSQVLTDKGTFYIDENMYKNIVDSMDRDADGNLYGNAAGSYVS
                     LEVGNLYRFKLDGDKIYRVSDSLREVDNISVASTAGNQITYKKGFKTYSITLPSRANY
                     YHNGTKQNYNDINKIIESASSIILEYNEDKSYYDNVLIFDPVYSKPEASYKFDYTSKK
                     LGSIDFPDNPTIVKNGRVIGISEIEDYDVVYKVTDIWNEAGYILVVDKKIEGKITNIL
                     PNKLSPQKIEIDNVVYELGKDMMFSKINSTPGSFKVDDSIVALFNYEGKVVDLLYKGE
                     ENNAHYAFVLNSNSITSTDAADYGRVEYYAKMLFTGGTIATCRVKYNAAENKGRLVKF
                     TKNDDNIYSISEVNYISTLDMKELAVDKDEGRIGDDYVTENIKIFNIETNETGKDAEV
                     HVLNWTDMKSGNIILGKTLYINKVGDFNDINLMVVNDMFNQKYKFALIKDIQSSGTGA
                     GAAGAGAGAGSSKTTPYNYKLLIEGKEYSYSDTLYFTKDSVVRVSMANNTVKEIMDIR
                     SRDVLTDNIQAVDNKRIKIKDKIYRFKDTTAIYLKKADGEYIRIGTNDIDTSKKYLLI
                     SVYLDKAYQNGGRAEVIVIVERAEE"
     gene            2643..4499
                     /locus_tag="PK733_06050"
     CDS             2643..4499
                     /locus_tag="PK733_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOJ10139.1"
                     /translation="MSKNSLIIKEKPGANRELSVGKTILRTIAKTITKTIVKTAAVLC
                     SILITTNMAMTASSSAIEPYTLLSDTSEYKFNMSYLYFGDPSTYVNSIENTRNSITEI
                     SPSYFDLNTNGTLKITSKIDKNFINEMHNRGIKVVPFLSNHWERNLGRIALNNRLVLV
                     EQIVNAIRDYNLDGINVDLENLTEEDRDKYTDFVKLLKGKLPADKIVAVAVASNPEGF
                     TKGWHGSYDYRALGQYSDYLMVMAYDEHYGGGPAGPVASYSFVERSVKYTLDRVSREK
                     VVLGIPFFGRIWKQGGGLVGQGVSLTTVEDLISKYRGKTSFDIASLSPKAVITIRPED
                     EKPYIFGIRLDAGTYTIWYENEESIKYKLGLVNKYGLKGTGSWSLGQETSSTWDYYEL
                     WLNGHYFEDSQGHWAQKDITFVANKGWMIGDGWTSFAPNNTLTRAEAATALVRSLGLK
                     EIVSQENEIAFNDISGHWAEKYIRIAAQNKIVFGRGDNSYGPDVEVTREEIAVMMDRI
                     LADKGFSNIQLANREQGQIASRGGSSGGSLGNSSGNSPGSSIDTAARYYQISGKTYKD
                     VNTETCAWSYDSIIRMTQIGILTGAPDGRFLPKNIARRAEMAALLCRISGKI"
     gene            4591..4666
                     /locus_tag="PK733_06055"
     tRNA            4591..4666
                     /locus_tag="PK733_06055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4624..4626,aa:Thr,seq:cgt)
     gene            complement(4960..5430)
                     /locus_tag="PK733_06060"
     CDS             complement(4960..5430)
                     /locus_tag="PK733_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ10140.1"
                     /translation="MAFFDNLGKKVGEAAQAAAKKSGELVEVTKLNMSISTEEDKIKK
                     LYSKIGENTYAKFRSASEIDPDFIADCEEIESHEEAIKNIRAKIMEIKNVKACMGCGA
                     ELDRNAMFCPKCGAKQEPVQAAETQSATSVFCQKCGAAVTDGSAFCQSCGAKIE"
     gene            complement(5513..6859)
                     /locus_tag="PK733_06065"
     CDS             complement(5513..6859)
                     /locus_tag="PK733_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10141.1"
                     /translation="MIKKHAKYFALVVAVCIAISTVPSYNRPQAGALADLFDIKDDTG
                     GFVVDNTYDYFAIGKLSDWGESTGNFYVSVGQGADLTKYRPEKAAELINQGNKIINLG
                     NGLRAGGQILNIYNTAVDTYKLFTEESKHETMVEKGIDKTLFVVDVGMGWYAVGAGAI
                     ALFTAPAWGAGLVAAAGATGTTYLVSKLGVGAARIAFNSETYRNASRYVRNTVSDAID
                     LATGKKKLVFAPLQRPGIKEGLDIIEEELGFDLYPGLRREIPDPSTGIPVYKPNIYLY
                     SDANMSVNVKLYPGHWITLSDPIYPENEGWTAQIVNGSLNGKGDYLFYEALVPDSDFQ
                     KKNGWKIRWNSRRNDIIKILDKYSFNEKEKSDFLEYWTEKLVHSKDYIFYPQENIQVD
                     TIMPVILSKEPDNIFRIWFYIEPNEGQDTLEPEQNMVINRNGFTVVEWGGILASEH"
     gene            complement(6852..7880)
                     /locus_tag="PK733_06070"
     CDS             complement(6852..7880)
                     /locus_tag="PK733_06070"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ10142.1"
                     /translation="MWGIGILLLLGTLIQIFISGASLLNIVLLIVSGLFFLSGLPFMR
                     KLLSGIGSKGQKDTPGTANIHGAGGAGSVAVTPDAPGTPCTPCTPGTPGTPGTPGIPG
                     TPGIPGTHSAHNSHGTSGVAGILVPIVLIVCFLVAGALFNPFKEERELSSNYSKGMKY
                     LSHKEYEKAESTFSEMEAKKPQDDQVLLAKALVYLNMKSYDKAWDYLNRAYQANPYDL
                     NIQYNMALVRYHLDDKADALNRFVSILQTNPRILRPRLYAGTISMELGNYPKAIYHLE
                     QARWFAPDSIEVQYNLGKSHYAIMEYADAKAAFENALSLKPEKEVEANIKKALEDINR
                     YLGGDVND"
     gene            complement(7881..10385)
                     /locus_tag="PK733_06075"
     CDS             complement(7881..10385)
                     /locus_tag="PK733_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ10143.1"
                     /translation="MFCSKHGAGIEGANNFCIECGGFAWLVLAIFILQGSFLIKSILL
                     VINQKTISFIWTPVSSIITYLLFIFVFIIYIKSINRELLSTNISTTSSHIDFMYRCAS
                     LARDFITLLTSSFSTLLFLWGVFSGSGLIISRLYSLVVKAGMDNPGQGMLILAGYAML
                     VLLMQFLLIEGLFLLYISARYRLTFFKTGKALDRINIWMLKHLPRMVILDLALSAMIV
                     LLYFFQNFGGNIIQNIQPYGFLQTFLKYLLSSVLAAAILAVIFSQASKMAGDNRESFE
                     AIANSNFRSIPVIPVIFALIAAASVFILPIQPQDGYASIIADIRGHSSKGDILRDAGY
                     MTGAIKEYDDALSKLYSLQAYLLTRKSIISGNASLRNESSKLMASAKALDLLNGYAAL
                     FEGRISLEENQPSIAVNTFRKGIKNPLAIPETFAGLLEASIPAQNKEVKEMSIDWLLG
                     NEIYHDKFAALGKASLKKLDKWIEQLNEMQKDLEPNLVYKYYEKTMYYEHQEALNGLL
                     SLKEKYPDNGEVNYLLTRTLAEFRNEQNNYSMLIDSAKAFSNSIENYEDRDVLLAKDS
                     YSAKIHMLANDYVTSEKIFDDLYRKYPDSRETAETYLYLLIKNQKFNDALKLAKELEK
                     GEKNPTLVYNHSICLLNMNNYAESLSKIKEIEKYSEPYSEEYDRYLYAYSLAYVNAIK
                     FQEHVDLLENQLGDALIKYYILAMKGWKERNSEESNLQLNKIIAKDKRLGYVWYCKGV
                     NAYENAVRTNSGNFEDSIKYYLKSLQILPEHVEGYFALGHCYNKAGMKEEALRAFRKV
                     VRLVPYEDHRVDPYGMVVHAWGEIDRLSNELERGNR"
     gene            10756..12879
                     /locus_tag="PK733_06080"
     CDS             10756..12879
                     /locus_tag="PK733_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOJ10144.1"
                     /translation="MKKFLSIILVALLVFATFVSPVSADSKHNKNDENLKKKYMNELK
                     TLKGQLKCFEKQYQERLKIMKQLEKLKKKYNDKHNYYFDDDFDLEDLWLLINGEEFLW
                     KGTPVIRYGSFLLPIKPITEGLKADLKYDDKKHVISITKGDTKIVIDLEGKVITVNGE
                     KVNWDIFNKKSSNGTIVLVKVIATVLGHDVDGDDDSGVIIIDDKYTSFNNNDVRLEYK
                     GSWTYLQNQKDAYMGDLHVSNDKDASFKFSFNGTKIKLYGVKGPDKGNAIIKLDGKEQ
                     TVNLFSYTTQNNAIIYASTELKEGPHTLEVKVSGSKYFWSKGYSVAVDRVDVMGESVK
                     DWNIAINKPASADSHEVNNTPQKANDGSLSTRWCAADDATGHWWMVDLGGYYNLTGTE
                     IVWEFDEKLYKYKVDVSANGSDWTLALDKANNNLKQQVQNEKFSANSVKYVRVTVTGL
                     EPGCWASFYETKIFGYPASASQPQVPGAPESLTAKSVSDTEILLSWKAPTLNAWLSGY
                     KIYRNGTHIGTVGTTANNLTYKDTGLTPGTAYEYTIRAYNSQNVNSSPSSTLKVSTFG
                     NGTGLKAEYYDKVDFTEQKLLKSDDLINFIWGSNPPNTYITDSTFSVRWSGKVQPLYS
                     GKYTFYTLSDGGVHVWVNDSKVIDDWTEHAISEKSGTIELTAGNKYDIKVEYYNESGS
                     SQIQLFWSGDSQQKEIIPKRQFYID"
     gene            13213..14946
                     /locus_tag="PK733_06085"
     CDS             13213..14946
                     /locus_tag="PK733_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ10145.1"
                     /translation="MVNMLVKKIFSSYKQMEQLNKIMFFYRYFSLFITSVFFLANYKQ
                     HTTWIAIIVIGGFSISAIILNYLYSINANHKNFIKILVLIETVGNALTLIPTGGLNSP
                     YLWYSLNTVLVTLAFLNFKYCFLNLMIYIIITTKISHILFNNKDISFWSVLANNSNLV
                     LSFILITVVGHLLISLLNTLEEERNKLINVNDQLNLTNKNLKKSIEYIMSINHAVHNL
                     VNQHDEVRLIKLLIEYSIDMTKTNRAFFISLFGQLIDYEVKDRYEEVFLTEECKNIIV
                     NQKDKIINSFTPLNMEVNGKNLIVVEVKSLYQSYGILGVAVEFNDSVDLGDSDILYEE
                     LSSQMKLLSELGSIVFERYQLQDTNNRLLINDEQNRIANEVHDTVLQRLFSISCAIQT
                     LLQKQCNTRLSDIQGELIVMKDSLVSSMKELREIVYKLSWRKNGENAFLNDISRYIDE
                     ISKLTGVRILFSSSGDQALLSCDMKRILCRITREAVGNAIRHSKCSNINVSIDIDDSS
                     TKVEIKDNGRGFEINKDFSSTGLGLKNIYNLINSSGGKIDIASQCGIGTVMSAVIPNN
                     SFKDVIKGEAV"
     gene            14943..15566
                     /locus_tag="PK733_06090"
     CDS             14943..15566
                     /locus_tag="PK733_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10146.1"
                     /translation="MKLIIVDDHPLVRKGINSILSTCNDIDEVYEADNIASAMELIIN
                     EKPHIVLVDLRLGSQNGLELVEKAKQANLDTKFIVLTSCLLHDDFLRGEKIGVDGYIL
                     KEAFAEDIMYAIRLVQRGKKYYDSTIMGHKNELDGEGLLLNTLTERENEVLREIGAGM
                     TNEGIAKKLFISQHTVKKHVSSILSKLELNHRTEAALYINKALKVRY"
     gene            15664..16221
                     /locus_tag="PK733_06095"
     CDS             15664..16221
                     /locus_tag="PK733_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10147.1"
                     /translation="MTKKRRRKKHVSNSLVIVSAIITLNFLGISYAIWNESLSMNVSV
                     STGYIDPYFSSCIIVDKIPGTGNGLTAIISEDGHSIEIMGTVNRGYEGELHYHVSDSG
                     SVPTNYCGDYLMVIKPEAAIEAKPEEANNIEKTYIKSKETNAEGTNTDDANVEKTNVE
                     KTNVEEIDIKEEEIYEFTNILQFSL"
     gene            16253..16747
                     /locus_tag="PK733_06100"
     CDS             16253..16747
                     /locus_tag="PK733_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10148.1"
                     /translation="MKKKIIILFNIIIAFIFVAEGYSTWEKTLTIHGSIVVAESKKAI
                     TEDISKTNLPRQAAAHEKAEGDKIEVKEAAEGEETEKAIEGEEREEATEGDEVAEGDR
                     RDASEGGNTEIVEDENIDIIEEENADLVEEEKIVKEEGEKTHDNEESNHTKDEEDNKN
                     NDNN"
     gene            16791..17993
                     /locus_tag="PK733_06105"
     CDS             16791..17993
                     /locus_tag="PK733_06105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ10149.1"
                     /translation="MSKKYLPVKVSKTRCFLLLGVFLCLYFMDNLPIASFIGYNLYNN
                     YLKPALWLGIALTIWKLPRIRYSAKLRFRQGLILWAFSFAVIYIFISIGAGLIDGFGK
                     SPYNHSFKGMLTNIFVIGSALIGCETLRSYVVNSMTHKKVNYFVFLLISILITICNFS
                     TNIFTGLKGIKDIVVFFAQFFAPEFSHNLMATYLAFLGGTIPAITYTGILQAFHWLSP
                     VLPNLKWITTALIGILCPVFFLLSMQNIYLKESGTYPKKDKREDSIASWMITSLVSIF
                     VIWFAVGVFPVYPSVIATGSMEPMIKPGDVILIDKSVDKSKLNVGDIIQFRRDNILIS
                     HRIVEIIDDDIGKGYLTKGDNNSARDSNVVRLDKIKGKLIKVVPKVGWLTLLIKSERD
                     IQLDGIEF"
     gene            18255..18902
                     /locus_tag="PK733_06110"
     CDS             18255..18902
                     /locus_tag="PK733_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10150.1"
                     /translation="MKKTKFLVLALAVAIMMMGAGYAAWSETLKLDATVNSTYLNIVL
                     GRATETTYVRDTIDDSALEGYGTKCKVYLYDNAGGANTNSSRAEVYFVNLFPGIKQKA
                     TITYKNDSKIPVKLTKVVYSQFTDNGLLTASIPATVMLQTVNMNNGSWPLQSLAGKTY
                     THNGEGSTPVINPEVVLQPGEVMTYIIEIELPIESENDSQLKNLSFDVTTTFEQL"
     gene            19025..20110
                     /locus_tag="PK733_06115"
     CDS             19025..20110
                     /locus_tag="PK733_06115"
                     /inference="COORDINATES: protein motif:HMM:NF036452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5305 family protein"
                     /protein_id="HOJ10151.1"
                     /translation="MKLKITKTLKAILLVINVILIIGVVIYFYRYYTSPHSIEETIVK
                     YSYIQKGDIKYQVQLIPNDLYEERSIDEGSIYITQLIDCIKVSCNYNFEGDKSADIKG
                     DYEIAAVVEGYVGEKDSVKTIWKKKFPIIPRTTIEAQKEGISIDKEVLFKLDEYREFA
                     RVIIEILKVNTNAKLCIQMNVNLKADIDKISVQEKMSPAIEIPLNVNYFTISKLNIAE
                     KPGKVEETNRVKVPPDKKIIILCGIIIGILIISLLYLIIFTTEPTIKDKDIKNFNKIF
                     KNHGNRLVALSNKFVTANQRCCRVKSIDDLVRIADELGKPIFYEHSRNFVEITRFFVF
                     DDTWLYILDIKDSTYNEVIKEPDKGLI"
     gene            20306..20908
                     /locus_tag="PK733_06120"
     CDS             20306..20908
                     /locus_tag="PK733_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10152.1"
                     /translation="MRTEKRVYLFRLIDISLFAFVGVWLLYSLLNITFANENQATPNV
                     IVTINTNGSISNHGSLFGDHLWYPGKEESGIIRIQNNYKKIRVTGMGLNISLNGYQQE
                     YGEEMVMASFLKNMKLIVKKGKFLYFNKVILDNIKFLDLVNNGINDIFNGLVLEESDS
                     FNIGKSDSIDLKYTIRMDEDAGAEMQSLSANIQFIIDFGE"
     gene            complement(20948..21781)
                     /locus_tag="PK733_06125"
     CDS             complement(20948..21781)
                     /locus_tag="PK733_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ10153.1"
                     /translation="MSEYNIKTPEVNKIIDVHAHIFPDKVAEKAVENIGSYYGIPMYG
                     KGTVSDLFESGGKIGVYRYIVHSTATKPEQVKHINNYIASAQASNSCITGFGTLHPEL
                     KNTEIKTEVERLQSLGISGIKLHPDFQGFDIDQENMIPIYSAIEGKLPLLIHMGDKNK
                     TSSSPKRLARILKMFPGLTVIAAHMGGYTMWDESMEYLVGKNVYFDTSSTLMFLDKSR
                     AVEMIRAHGAGKVLFGTDYPMWSHQDELQRLHDLGLSADELELIFWKNASGILGINLS
                     A"
BASE COUNT         7549 a         3450 c         4212 g         6771 t
ORIGIN      
        1 ggtatccagc taataaagct tcaaaactca accctataga ggctcttcga gttgagtaac
       61 catatgggag gtatagaaat gaaaagattt atagcacttg gtttatcaat aatattattg
      121 tgtgctgcaa tgaatgtttt tgcagctgca ggaacagtcg gttcagtggt tggagcagat
      181 acatacgata aagcttccta ttacaatact tttaaggaat taaaagtcct tggaatacta
      241 aaatattgtg acaccagtct gcttgctcca ggcaatttgc tcacaagaaa acaatttgcg
      301 ggaatactta caaagctggc agggctggac gaatacccag acttgttaat aggtgcgcct
      361 gattttaagg atgtagatcc taaagaccca tctggtaaat gtataaaagc tgtttataaa
      421 aaagacctta ttacaagcat gcctgacgga atgtttcatc ctgatgaacc tgtaaccttt
      481 gctcagtttt gtaccgctgc agttaaattg cttggatatg gtgacggtga tattatcgga
      541 ttatggccca aaaactacat tgataaggct caatcactgg aaataacaaa aggactgaat
      601 tttagacatg gagatgagct gccgatttgg actgcgtttc atattattca aatattattt
      661 gatactgaaa ttaaaaaaac agcatctgat caagtatcaa agacatatgc agaaaagatg
      721 ggattttatg aagaattttt ggtactggca gattccaata tattacaaac ccttgataag
      781 tctcaggtat taacagataa aggcactttc tatatagatg aaaatatgta taaaaatata
      841 gttgatagta tggatagaga tgcggatgga aatttatatg gaaatgcagc tggaagttat
      901 gtatctttag aagtcggaaa tttatatcga tttaagctgg atggggataa aatatacaga
      961 gtatcagata gccttcgtga agttgataat atctctgttg caagcactgc aggaaaccaa
     1021 attacgtata aaaagggttt taaaacatac agcatcacgc tgccttccag ggcaaactac
     1081 tatcataatg gcacaaaaca aaattataat gatatcaaca aaattataga gtctgcttct
     1141 tcgattattc ttgaatataa tgaagataaa tcttattatg ataatgtttt gatattcgat
     1201 cctgtataca gtaaaccgga agcatcgtac aaatttgatt atacatctaa aaagcttggc
     1261 agcattgatt tcccggataa tcctaccata gttaaaaacg gcagggtcat tgggatttct
     1321 gaaattgaag attatgatgt agtatataaa gtaacagata tatggaatga agcagggtac
     1381 attcttgttg tagataaaaa gattgaggga aaaataacca atatacttcc gaataagctt
     1441 tctccccaaa aaattgaaat cgacaatgta gtttatgagc ttggcaaaga tatgatgttt
     1501 agcaagatta acagcacacc aggttctttt aaagtggatg atagtatagt agctttattc
     1561 aattatgaag gtaaagttgt tgatcttttg tacaaaggag aagaaaacaa tgcccattat
     1621 gcctttgtgc ttaacagcaa ttcaataacg tccacagatg cagcagatta tggcagggta
     1681 gagtattatg caaaaatgtt gtttactggt ggaactatag caacttgtag agtaaaatac
     1741 aacgctgcag aaaataaagg cagattagtt aagtttacta aaaatgatga caatatctat
     1801 agcattagtg aagttaatta tatcagtaca ttagatatga aggagcttgc ggttgataag
     1861 gatgagggga gaataggaga tgattatgtt actgaaaata taaaaatatt taatatagaa
     1921 actaatgaga caggtaaaga tgcagaggtg catgttctca actggactga catgaaaagt
     1981 gggaatatta ttttaggcaa gaccctttat attaataaag ttggagattt caacgacata
     2041 aacttgatgg tcgtaaatga catgtttaat cagaaataca agtttgctct tataaaggat
     2101 attcaatctt cagggacagg tgcaggggca gcaggagcag gtgctggagc cggtagttcg
     2161 aaaaccacac cttacaatta taagctgtta atagagggaa aagaatattc ttacagtgat
     2221 actctatatt ttacaaaaga ttcagtagta agagtaagca tggcaaacaa cacagtaaaa
     2281 gagataatgg atattagaag tcgtgatgta ctaacagata atattcaggc agtagataat
     2341 aaaagaatta agataaagga taagatatac cggttcaagg atactaccgc tatttattta
     2401 aagaaggctg atggagaata tatacggatt ggtaccaatg atatagatac ttcaaagaaa
     2461 tatttactga tttctgtgta tctggacaag gcttatcaaa atggtggaag ggcagaggta
     2521 atagttattg tagagcgagc tgaggagtag tatgggagca tgaaatttta gtttacataa
     2581 tttgtgcatt gaatttttga caaatatcct gtaatctatg taataggggg gttacaggta
     2641 ttatgtctaa aaacagctta attattaaag aaaaaccagg tgcaaataga gaattatctg
     2701 ttggaaaaac aatattaaga acaatagcaa aaacaataac aaaaacaata gtaaaaacgg
     2761 ctgcagtact atgcagtatt ttaataacga ctaatatggc tatgacagca tcatcatctg
     2821 caattgaacc ttatacatta ttatctgata catcggaata taaatttaat atgtcatatt
     2881 tatattttgg agatccttca acttatgtaa acagtattga aaacactagg aattctataa
     2941 ctgaaatttc tccaagttat tttgatctta atacaaatgg tactttaaag attacatcca
     3001 aaatcgataa aaactttatc aatgaaatgc ataatagagg tattaaggta gtaccttttc
     3061 taagcaacca ttgggagcgg aatttgggaa gaattgcatt gaacaacagg ttggttttgg
     3121 tcgaacaaat tgttaatgcc ataagagact acaaccttga tggtataaat gtagatttgg
     3181 aaaatctaac tgaagaagac cgagataaat atactgattt tgtaaagcta ttgaaaggaa
     3241 aattacctgc cgataaaatt gtagcagtgg ctgttgctag taaccctgaa ggattcacaa
     3301 aaggttggca tggttcttat gattatcggg cattgggcca atacagcgat tatttaatgg
     3361 ttatggcata tgatgagcat tatgggggcg gacctgcagg gcctgttgcc agctatagtt
     3421 ttgttgaacg ctctgtaaaa tatacattgg atagggtctc aagggaaaag gttgttttag
     3481 gcataccttt tttcggacgc atatggaaac agggtggagg actggtggga caaggggtga
     3541 gtcttactac tgtagaagac ctgatttcaa aatatagagg aaaaacttct ttcgatattg
     3601 cttctctttc tcctaaggct gttataacca ttagacctga ggatgaaaaa ccatacattt
     3661 tcggaataag gttggatgca ggaacataca caatatggta tgaaaatgaa gaatctataa
     3721 aatataaatt agggctggtt aataaatatg gacttaaagg cactgggagc tggagcctgg
     3781 gacaagaaac atcaagtaca tgggattatt atgagctttg gcttaatgga cattattttg
     3841 aggattccca gggacattgg gcacaaaaag atattacatt cgttgcaaat aaaggctgga
     3901 tgataggaga tgggtggaca agctttgcac ccaataatac attaacaagg gctgaagctg
     3961 caactgcact ggtgcgcagt ctgggactta aagaaattgt atcacaggaa aatgagatag
     4021 cttttaatga tatatcaggt cattgggcag aaaaatatat aagaattgct gcacagaata
     4081 aaatcgtatt cggacgtggg gataattctt atggccctga tgtagaagtg acccgtgagg
     4141 aaatagctgt tatgatggat agaattttgg cggacaaagg gttttcaaac atacaactgg
     4201 caaataggga acaggggcag attgcaagcc gtgggggcag tagtggaggc agtcttggga
     4261 atagtagtgg aaatagtcct ggtagcagta ttgatactgc tgcccgctac tatcagatat
     4321 ccggaaaaac ttataaggat gttaacactg aaacatgtgc atggtcatat gattctatta
     4381 ttagaatgac acaaatcggc attttaactg gagcacccga tggcaggttc ctacctaaaa
     4441 atatcgccag gcgtgcagaa atggcggctt tgctatgtag aatttcaggg aaaatataga
     4501 gataacagta tactaccaac ttgcgaaaaa ccagtattga caaagacttt aacttgatat
     4561 aatatttaac aaggtggtta aagtagtgat gctactgtag cgcagtaggt agcgcaattg
     4621 attcgtaatc aataggccag gggttcgaat cccctcagta gctccaaaaa aatgtcttgg
     4681 ataaatcttg tataaatctt gcataaatag gaaaaagaca ctcaattata aaagccctga
     4741 tgatgaaaca aaagtttata gaaacatggt agggctaaga aatttattat ctttgtccat
     4801 gataattttg tttaaaatgc aatgctataa tgtaatgcta taaggatggt cttttcttat
     4861 gctgagtacc tacatagcag tgctaatatc ataccctgca aattaaaaaa gcagatgata
     4921 taactatatc atctgctttt tttaagtaaa ataaacattt tactctattt ttgcaccgca
     4981 gctctggcaa aatgctgatc catcagttac agcggctcca catttttgac agaatacact
     5041 tgtggcgctt tgggtttcag cggcttgcac aggttcttgt tttgcaccgc attttggaca
     5101 gaacattgca tttctatcca attctgcacc gcaacccata catgctttca cgtttttaat
     5161 ttccataatt tttgctctga tattttttat tgcttcttca tgactctcaa tctcttcaca
     5221 gtcagcaata aaatcagggt ctatttcgga ggcagaacgg aattttgcat atgtattctc
     5281 accgatttta gaatacagct ttttaatctt atcctcttct gtactgatac tcatgtttaa
     5341 ttttgttact tctacaagct caccggattt ttttgcagct gcctgagcag cttcaccaac
     5401 ttttttacct aagttatcaa aaaatgccat attatttcat cctcccatct ttaaattaat
     5461 gttaaataaa ttattattta ttatataggg atttcgcttg atgcgaaacc tattaatgct
     5521 cacttgctaa aattcctccc cattcaacta cagtaaaacc gtttctgttt attaccatat
     5581 tttgctcggg ttccaaagta tcctgtcctt cattaggttc tatgtagaac catatgcgga
     5641 aaatattatc aggttctttg gacaatataa cgggcattat agtatccacc tgaatgttct
     5701 cttggggata gaaaatatag tctttgctat gcaccagttt ttctgtccag tattcaagaa
     5761 aatctgattt ttccttttca ttgaaactat atttatctaa tatctttatt atatcatttc
     5821 ttctggaatt ccaccttatt ttccaaccat tttttttctg aaagtcacta tcgggaacta
     5881 aagcttcata gaaaagataa tctccctttc cattcaggct gccgtttaca atttgtgctg
     5941 tccaaccttc attttctgga taaatcggat cggaaagtgt tatccaatga ccagggtata
     6001 gttttacatt aacagacata ttagcatcag aataaagata gatgtttggc ttatatacag
     6061 ggatgcctgt ggaggggtct ggtatttctc ttcttaagcc gggataaaga tcaaagccaa
     6121 gttcttcctc gataatatca agaccttcct ttataccagg tctctgtaag ggagcaaaaa
     6181 ccagtttctt tttacctgtt gcaagatcaa tagcatcact gacagtatta cgtacatatc
     6241 tggaggcatt tctgtaagtc tctgagttaa atgctatacg ggctgcacca actccaagct
     6301 tggagaccag gtaggttgtg cctgttgcac ctgcagcagc aaccagcccg gcaccccatg
     6361 caggagcagt aaatagagcg attgcgcctg cgcctacagc ataccagccc attccaacat
     6421 caacaacaaa taatgtcttg tcaatgcctt tctctaccat ggtttcatgt tttgattctt
     6481 cggtaaagag tttgtaggtg tcaactgcgg tgttgtatat gtttaggatc tgtcctcctg
     6541 ctcttaagcc gttaccaagg ttgattatct tgttgccctg attaataagt tcggccgctt
     6601 tttcaggtct gtatttggta aggtctgcac cctgtcctac gcttacataa aaatttcctg
     6661 tactttcgcc ccagtccgag agtttgccta tagcgaaata gtcataggta ttatcaacaa
     6721 caaaaccgcc ggtatcgtcc tttatatcaa aaaggtctgc taaggcacct gcctgaggtc
     6781 tgttatatga aggtacggta ctaatggcaa tacaaacagc aacaacaagc gcaaaatatt
     6841 ttgcatgttt tttaatcatt tacatcaccc ccaagatacc ggttaatatc ctcaagagcc
     6901 ttctttatgt tagcttcaac ctctttttca ggctttagtg aaagggcatt ctcaaaagca
     6961 gctttagcat ctgcatattc cataattgca taatgacttt tacccagatt atattgtact
     7021 tcaattgaat cgggtgcaaa ccatcgtgcc tgttcaaggt gatatatagc ctttggataa
     7081 ttaccaagct ccatgcttat agtcccggca tacaacctgg ggcgcaatat tctgggattt
     7141 gtctggagaa tgctaacgaa tctgtttaga gcatctgctt tatcatccag gtgatatcgt
     7201 accagcgcca tattatattg aatattcaaa tcatatggat ttgcctggta agcacggttc
     7261 aaataatccc atgctttatc gtagcttttc atgtttaaat ataccaaggc cttggcaaga
     7321 agtacctggt catcctgagg cttttttgct tccatttccg agaatgtgct ttcagccttt
     7381 tcatattcct tatgggagag atacttcatt cctttgctgt aatttgatga caattcacgt
     7441 tcttccttaa aggggttaaa gagagcacca gcaacaagga agcagacaat aagaacaata
     7501 ggtacaagaa taccagcaac accggaagta ccatgagagt tatgagcgct gtgagtgcca
     7561 ggaataccag gagtaccagg aataccagga gtaccaggag taccaggagt accaggagta
     7621 caaggagtac aaggagtacc aggcgcatca ggagtaacgg caacacttcc agcaccgcca
     7681 gcgccatgaa tattagcagt accaggggta tctttttgac ccttcgaacc aatgcctgaa
     7741 agaagttttc tcataaaagg cagtcctgag agaaaaaaca gtcctgaaac tatcaaaagt
     7801 acaatgttta gcaaactggc accagaaata aatatctgaa taagggttcc aagtaagagt
     7861 aaaataccta taccccacat ttaacgattc cccctttcca attcgtttga aagcctgtct
     7921 atttctcccc aagcatgtac taccatccca taaggatcca cgcgatgatc ttcgtatgga
     7981 acaagccgga ctaccttccg gaatgctctc agtgcctctt ccttcattcc tgctttattg
     8041 tagcagtggc caagggcaaa atacccttct acatgctcag gcagtatttg cagagattta
     8101 agataatact tgattgaatc ctcaaaattt ccactgttgg ttcgcactgc attttcataa
     8161 gcattgacgc ctttgcaata ccatacataa cccagccgct tatcttttgc aattatttta
     8221 ttcagctgca gattcgattc ttcactgttt ctttccttcc agcccttcat agccaggatg
     8281 tagtacttaa taagtgcatc tccaagctga ttttcaagca ggtctacatg ctcttgaaat
     8341 tttatggcat ttacatatgc aagagaataa gcgtacaaat aacggtcata ttcttcactg
     8401 tacggttctg agtatttctc gatctctttt attttgctaa gggactctgc ataattgttc
     8461 atattgagaa ggcaaataga atggttataa accaatgtag ggtttttctc ccctttttcc
     8521 agttccttag ccagcttaag ggcatcgttg aatttctgat tttttattaa taaatacagg
     8581 tatgtttcag cagtttctct gctatccggg tattttctgt acaaatcatc aaaaattttc
     8641 tctgatgtta catagtcgtt ggccagcata tgtatttttg ccgaataact gtcttttgcc
     8701 agtaaaacgt cccgatcttc atagttctcg atggaatttg aaaatgcttt ggctgaatca
     8761 atcaacatgg aataattgtt ttgctcgttt ctaaactctg caagtgttct ggttaataaa
     8821 taatttactt cgccattatc cgggtacttt tcttttaagg agagaagccc gttcaaagct
     8881 tcctgatgtt catagtacat ggttttttcg taatatttat ataccaagtt aggttcaagg
     8941 tccttttgca tttcatttaa ctgttcgatc catttatcca gtttctttaa agaggccttg
     9001 cccagtgcag caaatttgtc gtgataaatt tcatttccaa gcagccagtc aatagacatt
     9061 tctttaactt ctttattttg ggctggaata gatgcttcaa gaagccctgc aaaggtttca
     9121 gggatggcaa ggggattttt tattcctttt ctaaatgtat taacagctat actaggttga
     9181 ttttcttcca aagaaatcct tccttcaaag agcgctgcat aaccgtttag tagatctaat
     9241 gcctttgcag aagccattaa tttgctagat tcattgcgca aggaagcatt accggatata
     9301 attgactttc tcgtcaaaag gtatgcttgc agagaatata actttgacaa tgcatcatca
     9361 tattctttga tggcaccggt catatatcct gcatccctga ggatatctcc tttcgaagaa
     9421 tggcccctta tatctgcaat tatacttgca tagccatcct gtggctggat tggtaatatg
     9481 aaaactgaag ctgctgcaat caaagcaaaa attaccggaa ttacaggtat ggaacggaaa
     9541 ttgctattag caattgcttc aaatgattcc cgattatctc cggccatttt tgaggcttgt
     9601 gaaaatataa cagccagaat tgccgctgcc agcactgaag atagtaaata tttcaggaaa
     9661 gtctggagaa aaccataggg ctgtatattt tgaataatat ttcctccaaa attctgaaag
     9721 aaatatagta aaacaatcat tgcactcaat gccaaatcaa gaataaccat gcgaggtaaa
     9781 tgttttagca tccatatatt aattcggtcc agtgctttgc cggtcttaaa aaatgttaac
     9841 ctatatcttg cggaaatgta taacaaaaac aacccctcta taagcaaaaa ctgcatgaga
     9901 agaacaagca tcgcatatcc tgcaagtatg agcatacctt gacccggatt atccattcct
     9961 gcttttacaa ccaggctgta aagcctgctt ataataaggc ctgaacccga gaatacaccc
    10021 cagagaaata gcaaagtact gaaactgctt gtcagcaaag tgataaagtc tcttgccagt
    10081 gatgcacacc tgtacataaa atcaatatgg cttgatgtag tactgatatt tgttgaaagc
    10141 aattccctat ttatactttt tatgtatata ataaaaacaa atataaatag taaatatgtt
    10201 atgatacttg atactggagt ccagataaaa gagatggttt tctgattaat taccagaaga
    10261 atgcttttta tcagaaaaga cccctgaagt ataaagatag ctaatacaag ccaggcaaac
    10321 cccccgcatt caatgcagaa attgttcgca ccttcaatcc ctgctccatg ttttgagcag
    10381 aacataattt attcccccta tctaagaaaa ctgcatcaat tgctgtataa aataatgatg
    10441 cgagttttct tacttattag aaaattggaa attaatacag acaaatatct tatatataat
    10501 attttatttt atatgataaa attctacaag tattttttca gtatatttaa aaaattttta
    10561 aatcattttg gaaagttttc aactgaaatt aaagcttttt tattgaccta aaaaccctgc
    10621 tcgatcatta cataaattac tataatatta caatcgtgtt aattttaaaa taactactct
    10681 tttccttacc ctattagcga atatatttat tgtaagtcat aaagcattaa aattaaattc
    10741 cgggaggtat tatgtatgaa aaaattttta agcattattt tagttgcact tcttgttttt
    10801 gcaacttttg ttagtcctgt tagtgcagat tccaaacata ataagaatga tgaaaacctg
    10861 aagaaaaaat atatgaatga gctgaaaacg ttgaaaggtc agttgaagtg ctttgagaag
    10921 caatatcagg aacggctcaa aattatgaaa caattagaaa aactaaaaaa gaagtataat
    10981 gataagcata attactattt tgatgatgat tttgatttgg aagatctgtg gcttcttatt
    11041 aatggtgagg aatttttatg gaaaggaacg ccggttatcc gttatggtag ttttctgctt
    11101 cctataaagc caattaccga gggattaaag gcagacttga agtatgatga taaaaaacat
    11161 gtaatttcaa ttacaaaagg agacacaaag attgtaattg atcttgaagg aaaagttatt
    11221 actgtaaatg gtgaaaaagt aaattgggat atttttaaca aaaaaagcag caatggaaca
    11281 attgtccttg taaaagttat tgcaacagtg ctgggacatg atgtggacgg tgatgatgat
    11341 tcgggtgtta taattattga tgacaaatat acttcattta acaacaatga tgtaaggctt
    11401 gaatacaaag gcagttggac atatctacaa aaccagaagg atgcttacat gggtgacctt
    11461 catgtatcta acgataaaga tgcttcattt aagtttagtt tcaatggaac aaaaataaag
    11521 ctttatggtg taaagggacc tgataaaggt aatgctatta ttaaattgga tggaaaagag
    11581 caaactgtaa atctattttc ctatactacc cagaataatg ctatcattta tgccagcaca
    11641 gaacttaaag aaggtcctca tactctggaa gtaaaagttt caggctctaa atatttctgg
    11701 tcaaaaggat acagtgtggc tgttgatcgt gttgatgtaa tgggcgaaag tgtaaaagat
    11761 tggaatatag ctataaataa gcctgcttcc gcagatagcc atgaagttaa taatacaccg
    11821 caaaaagcaa atgacggtag tctgtcaaca cgctggtgcg ctgcggatga tgccaccggc
    11881 cactggtgga tggttgatct ggggggatat tacaacttaa ctggtactga aattgtctgg
    11941 gagtttgatg agaagttata taaatataaa gtggatgtat ctgcaaatgg atctgattgg
    12001 acccttgcac tggataaagc aaacaataat ttaaaacagc aggtgcagaa tgagaagttt
    12061 tcagcaaact ccgtgaaata tgtacgggta actgtaactg gacttgaacc aggatgctgg
    12121 gcaagtttct atgagactaa gatatttggg tatcctgctt ctgccagcca gccacaggtt
    12181 cctggagcac ctgaatcttt aactgcaaaa tcagtctccg atactgaaat attgctgagt
    12241 tggaaggcac cgacacttaa tgcatggcta tccggataca agatataccg taacggaact
    12301 catattggta cagtaggaac tacagcaaac aacctgactt ataaagatac aggacttact
    12361 ccgggcacag cttatgagta tactattaga gcatataatt ctcaaaatgt taattcaagc
    12421 ccaagctcaa cattaaaagt atcaacattt ggaaacggaa ccggccttaa ggctgaatac
    12481 tatgataagg tagattttac agaacagaaa cttctgaaat cagatgattt aataaatttt
    12541 atttggggca gtaatcctcc taatacctac atcaccgata gcacattttc agttaggtgg
    12601 tcaggaaaag ttcaaccgct ttactcggga aaatatactt tttacaccct gtcggatggc
    12661 ggagttcatg tttgggtcaa tgacagcaaa gttattgatg actggacaga acatgctata
    12721 tccgaaaaga gtggaactat agaacttact gcgggaaata aatatgatat taaggttgag
    12781 tattataatg agagtggttc atcacagatt cagcttttct ggtctggcga ttcacagcaa
    12841 aaggaaataa ttcctaaacg tcaattttat atagattaag ctgtttgttc aggtcatgca
    12901 tttccagaag tctgctttga tagcccagac tttccgtaca gactcttgga aacgcatggg
    12961 gcctggctga ctaatcagcg gcaggataaa aaacccatgg gactataatt gtttgaatta
    13021 tagtcccatg ggttttttta taataactag caagttttag agtttggtaa catatatata
    13081 tactggtcaa gggtagtata ctgggtagta cgctgagcag tacgccggta acacaaaagt
    13141 attattgtaa acataagaaa attttgtatt cataatacat tagttaaata taaatttaga
    13201 tgtgagggaa gaatggttaa tatgcttgta aagaaaattt ttagctctta taaacagatg
    13261 gaacaactca ataaaataat gttcttttat agatattttt cattgtttat tacctctgta
    13321 ttctttctag ctaattataa acagcataca acatggatag caattatcgt aatcggtgga
    13381 ttttctattt ctgcaataat actaaattac ttatattcaa taaatgcaaa ccataaaaac
    13441 tttataaaga ttttagtctt aatagaaaca gtcgggaatg ctttaacact tattcccaca
    13501 ggagggctta acagtcccta tttatggtat tcacttaaca cagttcttgt aactcttgct
    13561 tttcttaatt ttaaatattg ctttttaaat cttatgatat acattattat taccactaaa
    13621 atatcacata tattatttaa caataaagac attagtttct ggtcagtttt agctaataac
    13681 tccaatcttg ttttaagctt cattttaata actgttgtag gtcatttact gatttcccta
    13741 ttaaatacat tggaagaaga gaggaataag ttgataaatg taaatgacca actgaatttg
    13801 actaataaga atttgaaaaa gtcaattgaa tatatcatgt caattaacca tgctgtacat
    13861 aatcttgtga atcaacatga tgaggtcagg ttgattaaac ttctgattga gtactcaata
    13921 gatatgacaa aaaccaatag agctttcttt atatctttat ttggacaatt aattgattat
    13981 gaggttaaag ataggtatga ggaagtcttt ttaacagaag aatgtaaaaa tattatagta
    14041 aaccaaaaag ataaaattat taattcattt acacctttaa acatggaagt caatgggaaa
    14101 aacctaattg tggtagaagt taagtcctta taccaatcat atggaattct aggggtagcc
    14161 gtagaattta atgattcagt tgatttgggc gattcggata ttttatatga agaattatca
    14221 agccagatga aattattatc cgaattgggt tccatagtgt ttgaaagata tcaactacaa
    14281 gatactaata accgcttatt gataaatgat gaacaaaaca ggattgcaaa tgaggtacat
    14341 gatactgttc tacaaagact ttttagtata tcctgtgcta tccaaactct cttacaaaaa
    14401 caatgtaaca ctagattatc tgatatacag ggcgagttaa ttgttatgaa agattcactt
    14461 gtaagttcta tgaaggaatt gcgagaaatt gtatataaac ttagttggag gaaaaatgga
    14521 gaaaatgcat ttctaaatga tatatcaaga tatatagatg aaatatcaaa acttacggga
    14581 gtaaggattt tattttcatc atcaggtgat caggcgcttt taagttgtga tatgaaaagg
    14641 attctatgca ggattactcg tgaagctgta ggaaatgcaa tacggcatag caagtgcagt
    14701 aacatcaatg tttctattga tatagatgat agctccacca aggttgaaat caaagataat
    14761 gggagaggtt tcgagattaa taaagatttt agcagtacag gactaggatt aaaaaatatt
    14821 tacaatttga taaattcatc aggtgggaaa attgatattg caagccaatg tggaataggt
    14881 actgtaatgt cagcagtaat accaaataac agttttaaag atgtaataaa gggggaagct
    14941 gtgtgaagct aattattgtt gatgatcatc cacttgtaag gaaggggata aattccattt
    15001 tatcaacatg caatgatatt gatgaggtat atgaggctga taatattgca agtgcaatgg
    15061 aattgataat taatgaaaaa cctcatatag ttcttgttga tttaaggtta ggctctcaga
    15121 acgggctgga actggtggaa aaagcaaagc aggcaaatct tgatacaaaa tttattgtac
    15181 ttacatcctg tttgcttcac gatgacttct taagaggtga aaagattggt gtagacggct
    15241 atattcttaa agaagctttc gcagaagaca ttatgtatgc tatcaggctt gttcaaagag
    15301 gcaaaaagta ttatgactca actataatgg gtcataagaa tgaactggat ggagaaggtc
    15361 tactgctgaa cacccttaca gaaagggaaa atgaggtgct aagagaaatt ggagcgggaa
    15421 tgaccaatga aggaatagct aaaaaattat ttatttctca acatacggta aaaaagcatg
    15481 ttagcagtat attatccaaa ctggagctaa accatagaac agaagcagca ttgtatatta
    15541 acaaagcatt aaaagtgagg tactaattga tatggtgaaa aggtataagt aattgtgttt
    15601 catattcttt atggcttttt atataatatc ttaattctag taaaatgatg gggtgatggc
    15661 gctatgacaa aaaaaagaag aagaaaaaag catgtaagca attctttagt tatagtatct
    15721 gcaattatta cactaaattt tttgggtata agttatgcaa tctggaatga aagcttaagt
    15781 atgaatgttt cagtgtctac aggttatatt gatccctatt ttagcagctg tataattgtt
    15841 gataaaatac cgggaacagg aaatgggctg actgcaataa tatcagaaga tggtcactca
    15901 atagaaatta tgggtacagt taatagaggg tatgagggag agctgcacta tcatgtttca
    15961 gatagtggga gtgtacctac aaactattgt ggtgactatt taatggtaat taaacccgag
    16021 gcagctatag aagcaaaacc agaagaagct aataatatcg aaaagacata tatcaaatct
    16081 aaagagacaa acgcagaagg gacaaataca gacgatgcaa atgtagaaaa gacaaatgta
    16141 gaaaagacaa atgtagaaga aatagatata aaagaagagg aaatatatga attcacaaat
    16201 attttgcaat ttagcctata aatcatatta ttgatataga ggaggtacgg ctgtgaaaaa
    16261 gaaaattatt atacttttta atattataat tgcatttata tttgtagctg aagggtatag
    16321 tacttgggaa aaaactttga ctatacatgg aagtattgtt gttgcggaat ctaaaaaagc
    16381 aattactgaa gatatatcta aaacaaatct cccgagacag gcagctgcac atgaaaaagc
    16441 tgaaggggac aaaatagaag tgaaagaagc cgctgaaggg gaagaaacag aaaaggctat
    16501 tgaaggggaa gaaagagaag aggctactga aggagacgaa gtagcagaag gagataggag
    16561 ggatgcaagt gaaggtggaa atacagaaat tgtagaagat gagaatatag acataatcga
    16621 agaagaaaat gcagacttag ttgaagaaga aaaaatagtc aaagaagaag gagaaaaaac
    16681 tcatgataat gaagaaagta atcatacaaa agatgaggaa gataacaaga ataatgataa
    16741 taattgaatg atgaattaat gaaggtaaat tttaataatg ggggtaagtg atgtctaaaa
    16801 aatatctacc agtcaaggta tctaaaacga gatgctttct gctgttagga gtatttctat
    16861 gtttatattt tatggacaac cttccaatag catcttttat cgggtacaat ctatataata
    16921 attatctaaa gcctgcactt tggcttggaa ttgctttaac catatggaaa cttcccagaa
    16981 taaggtattc ggcaaaatta agattcagac aaggtttaat attatgggca tttagttttg
    17041 cagttatata tatttttatc tcaattggtg caggattaat agatggtttt ggtaaaagtc
    17101 cctataacca ttcgtttaag ggtatgctta caaatatatt tgttattggt tctgcattaa
    17161 ttggctgcga aaccttaaga agttatgttg ttaatagcat gactcataaa aaagtgaatt
    17221 attttgtatt tttattaatt tcaatactaa taactatttg taatttttca acaaatatat
    17281 ttaccggact taaaggcatt aaagacatag ttgttttctt tgctcaattc tttgcacctg
    17341 aattttcaca taacctgatg gcaacttacc tggcttttct tggggggact attcctgcga
    17401 ttacctatac tggaatatta caggcgtttc actggctttc accggtgctt cccaatctga
    17461 aatggatcac aacagcacta ataggtattt tatgtcctgt attttttctt ctatctatgc
    17521 aaaatattta tttgaaagag tcaggtacat accccaaaaa ggacaaaaga gaggatagta
    17581 ttgcaagttg gatgataaca agccttgtgt caatttttgt tatttggttt gcagtaggag
    17641 tctttcctgt atatccttcc gtaattgcta caggaagcat ggagccaatg ataaaacccg
    17701 gagatgttat tttgattgat aaatccgttg ataaaagcaa attgaacgta ggggacatta
    17761 ttcaatttag aagagacaat atattaatat ctcatcgtat tgttgaaatt attgatgatg
    17821 atataggaaa aggttattta acaaaaggag ataataactc agcaagagat tctaatgtag
    17881 taaggttgga taaaataaag ggaaagctta taaaggtagt ccctaaagtt ggatggctaa
    17941 cattgctaat aaagagtgag agagatattc agttggacgg aattgagttt tagttttgaa
    18001 agtagatttc cgatagtaca aaggtagtat gaataatcaa taagtactac tattaattag
    18061 aactatagcg atactaaaca ttagactatt gagtggagta agaaaaaata atagttatta
    18121 taataaatat agtaaatata acacacataa aactttatat gccggctgaa aagtttttgc
    18181 atgttatatg ggtttttacc cagcctctaa gctttaacaa aataaaaaac ttgtttaaaa
    18241 ggagagattt ttttatgaaa aaaactaaat ttttagttct tgctcttgct gtagcaataa
    18301 tgatgatggg tgctggatat gcagcatgga gtgaaaccct taaattagat gcaactgtta
    18361 actcaacata tttgaatatt gttcttggta gagctactga aacaacttat gtaagagata
    18421 ccattgatga ttcagctcta gaaggttatg gcacaaaatg caaagtttac ttatatgata
    18481 atgcaggtgg cgcaaataca aatagcagca gagcagaagt ttattttgtt aacttattcc
    18541 cgggaataaa acaaaaagct acaatcacat ataaaaatga tagcaaaata cctgtaaagc
    18601 ttactaaagt agtttattcc caattcacag ataacggatt attaacagct agcatacctg
    18661 ctacagtaat gctccaaact gtgaacatga ataatggaag ttggccttta caaagtttag
    18721 caggtaaaac atatactcat aatggcgaag gtagtactcc tgtaattaat cctgaagtag
    18781 ttcttcaacc aggtgaagta atgacataca ttatagaaat agaattaccg attgaaagtg
    18841 aaaatgacag tcagttaaaa aatcttagct ttgatgtaac aacaacattc gagcaacttt
    18901 aataattact aataaaaaaa gcagaggcgt gcctctgctt tttttatgaa ataaatgaat
    18961 aaaaggaata aaactataat agtttatagt aaatactgaa gttctgggaa aggggttttt
    19021 actaatgaag ttaaaaatta ctaaaacact gaaagctatt ttattagtca taaacgttat
    19081 attaataata ggagtagtta tttattttta tcgatattat accagtccac attcaataga
    19141 ggagaccatt gttaaatata gctatattca aaaaggtgat attaaatatc aggttcagct
    19201 aatacctaat gatttatatg aagaaagaag tatagatgaa gggagtatat atattacgca
    19261 acttattgat tgcataaaag tttcatgcaa ttataatttt gaaggtgata agtctgcaga
    19321 tatcaaaggt gattatgaaa ttgcagctgt agtcgaggga tatgtagggg agaaagatag
    19381 tgtaaaaaca atatggaaga aaaaattccc tattatcccc aggacaacta ttgaggctca
    19441 aaaagaaggt atctctatag acaaggaagt tttattcaag cttgatgaat atagggaatt
    19501 tgccagggtt attattgaaa tcttgaaagt taacacaaat gctaaacttt gtattcagat
    19561 gaatgtaaac ttaaaagctg atatagataa aatttctgtg caggaaaaaa tgtctccggc
    19621 aatcgaaata cctttaaacg taaattattt tacaatatca aaattaaata ttgcagaaaa
    19681 acccggaaag gttgaagaaa ctaatcgggt aaaagttcct ccagataaga agataattat
    19741 actctgtgga ataattattg gaattttgat aatttctctt ttatacttga taatatttac
    19801 aactgaacca acaataaaag ataaagacat aaagaatttt aataagatat ttaaaaacca
    19861 tggcaaccgt ctagttgctt taagcaataa atttgtaact gcaaatcaac gttgttgcag
    19921 agttaagtcc attgatgatc ttgtaagaat tgcagatgaa ctgggaaaac ccattttcta
    19981 tgaacacagt cgaaatttcg tagagattac ccggttcttt gtttttgatg atacatggtt
    20041 gtacatttta gatataaagg attcaacata taatgaagta ataaaggagc ctgacaaagg
    20101 tttaatataa cgaatgatac acaaataaca caaaccgtaa aaaacaaatt gaacatgctt
    20161 ggaataacct atactactta gatagtacaa ttagtatact atggtattac taataattac
    20221 atatttgtat gaagataaaa gtagctgaat gagataatat aatatatata atatgtataa
    20281 gatctgattt tttgaggtga agccagtgag aacggagaaa agagtatatt tatttagact
    20341 tattgatatt tccctttttg cctttgttgg ggtatggtta ttgtattctt tactaaacat
    20401 tacatttgct aatgagaatc aggcaactcc taatgttata gtaacaatca atactaatgg
    20461 cagtatttca aaccatggaa gcctttttgg agatcaccta tggtatcctg gcaaagagga
    20521 aagtggaata ataagaattc aaaataacta taaaaagatc agggttacag gtatggggtt
    20581 aaatattagt cttaatggtt atcagcaaga atatggtgaa gaaatggtaa tggcttcctt
    20641 tttaaaaaat atgaagctta ttgtaaaaaa aggcaaattc ttatacttta ataaagtaat
    20701 actggacaat attaagtttt tagacctggt aaataacggt attaatgata tttttaatgg
    20761 gcttgtttta gaggaatctg acagttttaa tataggcaaa tctgattcaa tagatttaaa
    20821 gtatacaata cgcatggatg aagacgcagg tgcagagatg caaagtttga gtgcaaatat
    20881 acaattcatt attgattttg gagagtaaag tttaatacat accgctttat aattagtata
    20941 ttatggctta agccgataga tttataccca aaatcccgga tgcatttttc caaaagataa
    21001 gctccaattc atcagcagaa agacctaaat catgaagcct ttgcaattca tcctgatgag
    21061 accacattgg atagtcggta ccaaaaagga ctttcccggc tccatgagct ctaatcattt
    21121 ctacagctct ggacttatct aaaaacatta gtgtgcttga agtatcaaaa taaacatttt
    21181 tgccgacaag gtattccata gattcgtccc acatagtata gcctcccata tgagctgcta
    21241 taactgtaag ccccggaaac atttttaaaa tccttgcaag ccttttagga cttgaggaag
    21301 tcttgttttt atctcccata tgtattaata gtgggagctt cccttctata gcagagtaaa
    21361 taggaatcat attttcctga tctatatcaa atccctgaaa atccgggtga agtttgatac
    21421 ctgagatacc aagggactgc agcctttcca cttcagtttt gatttcagtg ttcttaagct
    21481 ccgggtgcaa agtgccaaaa cctgtaatac agctattgga tgcctgagca gaagcaatat
    21541 agttgtttat gtgctttacc tgttcaggtt ttgtggcagt agaatggacg atatatctgt
    21601 agactccaat tttaccgccg ctttcaaaaa ggtctgaaac agtacctttt ccatacatgg
    21661 gaataccata gtagcttcct atattttcaa ctgccttctc tgcaacttta tcaggaaata
    21721 tgtgggcatg tacgtcaatt attttattta cttcaggagt ctttatgttg tattcactca
    21781 agcttttcac cttcaaattc caatttgatt tcacgtttac aaggaattct accacacctt
    21841 gggtaaaagc acaagataca gggaaatgcg ccgagtgcat tttcttccta tgatacaaaa
    21901 tatggcagca tacgattttc cgagtataaa atttgaacaa agcttacgga ttgttatgtt
    21961 taaggcaagg aaaaattcgt aa
//
LOCUS       DAOSDD010000047        21945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1034_length_21945_cov_5.148150, whole genome shotgun sequence.
ACCESSION   DAOSDD010000047 DAOSDD010000000
VERSION     DAOSDD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21945
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1034_length_21945_cov_5.148150"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK733_06130"
     CDS             complement(<1..261)
                     /locus_tag="PK733_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOJ10154.1"
                     /translation="MKIAVIDGQGGGIGKAIVEKLVKELSDKKLTEDVNIITLGTNSS
                     ATAIMLKAGAKEGATGENAIIYNSRKVDVIIGPIGIIISNSMM"
     gene            complement(242..769)
                     /locus_tag="PK733_06135"
     CDS             complement(242..769)
                     /locus_tag="PK733_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10155.1"
                     /translation="MVSNTKKIALNGILGALTVICLLLAAILPTTKLSLYAFSSFFVS
                     IIIMEYKVAAGWAFYIATNLVAFIVVPDKIRIIPYTVFFGLYGIIKYYTERIKNAPLE
                     YILKYLYFNLCLAAAVFLVEKLFLVSENIKLPLWAAVIIFQFVFLLYDYAYTLFIKYY
                     ISRLRRIIKNENSSN"
     gene            complement(817..1455)
                     /locus_tag="PK733_06140"
     CDS             complement(817..1455)
                     /locus_tag="PK733_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ10156.1"
                     /translation="MNPYEAYKVLGIKEGADEEEIKRAYRELVKKYHPDQYRDNPLAK
                     LAEEKLREINEAYEVLSKQRTTGPKTNYSYGGYKNTNNTNTDSNNGYYRDDFFSNVRV
                     HINNGNIRTAEEILDSSSVRNAEWYYLKGLIFLRKGWQDEAYTHIQTAVNMDPGNLEY
                     RATLSRIYNAYSQRKQSGYGTNNDTDICSFCQCLICSDCCCECMGGDLIPCC"
     gene            complement(1442..2347)
                     /locus_tag="PK733_06145"
     CDS             complement(1442..2347)
                     /locus_tag="PK733_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOJ10157.1"
                     /translation="MFGYIMPEKPELKIKEYEMFKAFYCGVCKSIGKRIGQIPRVALN
                     YDSTFLAILLFSLSDSSISIIQERCMLHPVKKKNIAAENEIVDYAADMNIILAYYNLK
                     DDKIDNHNFVSSTGMFFLSPYYKKLKHKYKEKCDIIEKKLNELFELENRKCDSIDEVA
                     EPFSKIMEEVMAYEPLCADKKVEKMLRWMGYNLGKWIYILDAYDDMEEDARNKKYNPL
                     LVKYWNKNSEKNKINEEINQIKNEIKERIEFVLIYSLDQVIKTYELMDAKKVKGIIEN
                     IIYIGMLRKTEKILGTGSCRSFESI"
     gene            complement(2389..2910)
                     /gene="pgsA"
                     /locus_tag="PK733_06150"
     CDS             complement(2389..2910)
                     /gene="pgsA"
                     /locus_tag="PK733_06150"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOJ10158.1"
                     /translation="MNIPNLLTTIRFFLVPAFGYYVYERQYLLAVIIFTIAGLTDILD
                     GFIARKYNLITPWGKLADPAADKLMQLTALFMLNMQGKIPRAVVIIILVKEVTMIVGS
                     FFLYSKNKVVVQANWYGKLSTVILYVAIVMILFNIPFHDIFIFIALLFMLFSFFMYAA
                     DFLKKKNNKSDEA"
     gene            complement(2999..3418)
                     /locus_tag="PK733_06155"
     CDS             complement(2999..3418)
                     /locus_tag="PK733_06155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOJ10159.1"
                     /translation="MLESETRLVVRYAETDRMGIVHHSNYPIWFEAGRTDFIRKVGLP
                     YSKIEEEGIMLPLIELKCIYKSPAKYEDIIIVKTRINKLTLSRIIFNYEVFRENENTP
                     IAIGETIHVWTDTNLKPLNIKKHFPHIYELLIKTMQS"
     gene            complement(3656..3853)
                     /locus_tag="PK733_06160"
     CDS             complement(3656..3853)
                     /locus_tag="PK733_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10160.1"
                     /translation="MWTVVYMAQSKDIAENLQGLLTKEGILVKLRPISKNQESSSDYF
                     EILVPEAEVEEAHSVIIEKGF"
     gene            complement(3877..4110)
                     /gene="mtrB"
                     /locus_tag="PK733_06165"
     CDS             complement(3877..4110)
                     /gene="mtrB"
                     /locus_tag="PK733_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HOJ10161.1"
                     /translation="MDNEQIDAIGEYIVIKALENGVNIIGLTRGKDTKFHHTEKLDKG
                     EVLLAQFTENTSAIKIRGKAIIYCRHGEIHSGE"
     gene            complement(4178..7708)
                     /locus_tag="PK733_06170"
     CDS             complement(4178..7708)
                     /locus_tag="PK733_06170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ10162.1"
                     /translation="MNNLNKFVHLHIHTEYSLLDGAVRIKDLIAKVKELGMESVAITD
                     HGVMYGIIDFYKEAVENNIKPILGCEVYTARRSRFDKESGVDSEQGHLVLLAENNLGY
                     KNLMKIVSSGFIEGYYYKPRVDMEVLKKYNEGIIALSACLSGDIPTAILQGNYDRAKS
                     LALEYDKIFGRNNFYLELQVNNIENQNLVNQNLIKLSRETEIPLVATNDVHYLKREDA
                     RAHDILICIQTGKRINDEDRMKFNTEEVYLKSPEEMSALFKNIPDALENTVKIAKRCN
                     VELEFNVLHLPEYDVPGNQDHFEYLEKLCIEGLTRRYGPNCGNDKKERLDYELSVIKN
                     MGYVDYFLIVWDFIKFARDKGIMVGPGRGSGAGSIAAYCLGITNIDPLKYNLLFERFL
                     NPERISMPDFDVDFCYERRQEVIDYVIEKYGANRVSQIITFGTMAARAVIRDVGRALD
                     IPYGEVDMIAKMIPFKLGMTIDKALEINHDLMELYNKDEKIKDLIDTSRLFEGMPRHS
                     STHAAGVVISKEPVVEYVPLQKNDESITTQFTMGHLEELGMLKMDFLGLRTLTVLRDA
                     AELIKKNHGIIMNYDNMEMDDPKVYKMISEGRTTGIFQLESAGMTQFMKELKPSSLED
                     IIAGISLYRPGPMDQIPRYISNKINPEMVKYDHPLLENILNVTYGCMVYQEQVMQIVR
                     TLAGYSMGRADLVRRAMAKKKANIMKEERQNFIYGRTDENGNVLIKGAIRNGADEKTA
                     NKIFNEMMDFASYAFNKSHAAAYAVIAYQTAWLKYYYPTELMAALLNSFLGSTDKISR
                     YVNECKTMGIEVLSPDINESDIKFSVVNGKIRFGMAAVKNVGENAVKEIINERQKNGE
                     YISFRDFCERVSFRDVNKKCVESLIKCGAFDSMGIYRSKLILVFEKVIDSIQEIRRKN
                     LEGQLSIFDIPQKKDSPDIYETEIAYPDINEYPPNVLLAMEKEMLGLYVSGHPLEEFK
                     DLLSIHATANSLDFKEKAGESIDELEFDNSNDGVLEDGKKVIVGGIISEKKIKATKND
                     NLMAFVTLEDLYGRIEVIIFPQTLSMYQDIILEENIVLVKGRVSIKEEEEPKIICEEV
                     KPLTGDDISVGNKSGVYIIFDERPNNYSSNNRSLLSLLKFFKGKTPVHFYYRNEGISE
                     IVDTEYGINLNETLLSELKERMGNGNIKVV"
     gene            complement(7724..9466)
                     /locus_tag="PK733_06175"
     CDS             complement(7724..9466)
                     /locus_tag="PK733_06175"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOJ10163.1"
                     /translation="MKAYDKYLFWLENDYFDDVTKEELASIKDNMEEIEERFYRDLEF
                     GTAGLRGIIGAGTNRINKYIVRKVSQGIANYINKKGSNAAARGIVIAYDSRHKSPEFA
                     MEAALVFAGNGIGAWLFDELRPVPVLSFTIRYLKAAAGIAITASHNPKEYNGYKVYGE
                     DGAQLLPDESDLLLNEIDMIKDFSQVKIMEQGKALQTGLLQIIGKEIDDRYIDVLKTY
                     CLNPDIICKQSDTFKIIYTPLNGAGNKPVRRILNEIGFKNVFVVKEQEEPNPDFPTVK
                     TPNPEDESVFKIALEMAKRNDADLIIGTDPDSDRVGIMAKDNNGSYTSFTGNQIGCLM
                     LEYILSQKSLKGSLPSNGFIVKTIVTTELAKAIAKKYHIKVVEVLTGFKFIGEKIRLL
                     DENGDKKYLLGFEESYGYLAGTYARDKDAVSAAMIIAEMAAYYKSLGKTLMEVFEEIY
                     GNYGFTDVEVISFTLEGKEGLEQIQSIMEKLRKDSNLKLDGYEIYAIRDYLTGERYVL
                     KSGEREDLGLPESNVLYYELDDESWFCIRPSGTEPKIKIYIEVFGNDKTHAAEKLKDL
                     NGKVLDILNKCCSI"
     gene            9806..11284
                     /gene="spoIVA"
                     /locus_tag="PK733_06180"
     CDS             9806..11284
                     /gene="spoIVA"
                     /locus_tag="PK733_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOJ10164.1"
                     /translation="MESYNIYRQIAERTKGDIYIGVVGPVRTGKSTFIKRFMDLLVIP
                     NIENEYDRERAKDELPQSATGRTIMTTEPKFVPNEAIKIVLDENAQLRVRLVDCVGYL
                     VKGALGYLENNTPRMVSTPWFDHQIPFEEAAELGTRKVINEHSTIGLVVITDGSITEI
                     QRDEYLGAEKRVISELKAINKPFIIILNSTRPFAGETTKLKEELEEKYGVPVVNVNVA
                     QMRIEDVNNIMEKTLFEFPITEVGINTPKWIDALENDHWLRVKLLDSIREAFKGIRKI
                     REVKNAVSKFNGLEFIKKAYVEEINLGTGSVNIELSPPDGLFYRILSELTGLEIEGDH
                     RLIGLMKDLARIKKEYERIEYALHEVKVKGYGIVMPQVEDLSLEEPEIIRQGSRFGVK
                     LRASAPSVHMIRADIETEIAPLVGSEKQSEELVDYLLKEFENDPGKIWDSNIFGKSLH
                     DLVSEGLYNKLSRMPEDSQLKLQETLQRIINEGSGGLICIIL"
     gene            11544..12410
                     /gene="speD"
                     /locus_tag="PK733_06185"
     CDS             11544..12410
                     /gene="speD"
                     /locus_tag="PK733_06185"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOJ10165.1"
                     /translation="MKRTEPNKIKLHGFNNLTKTLSFNMYDICYTRSVSDRNAYISYI
                     DEQYNAERLTSILKHVTEIIGANILNIANQDYDPQGASVTLLVCEEPIGTVSNQNNAN
                     QNNTNIRGNTDNDNASNSNNTDNIRREAVIAHLDKSHITVHTYPEYHPDGGVCTFRAD
                     IDVSTCGKISPLNALNYLINSFEADIMSIDYRVRGFTRDITGRKIFNDHKFNSIQKYI
                     SNEVINHYHMIDVNVYQENIFHTKCKLKDFNLDNYLFGFSEKDIHPGEAKEITHRLQK
                     EMDEIFYGQNLY"
     gene            12562..14022
                     /locus_tag="PK733_06190"
     CDS             12562..14022
                     /locus_tag="PK733_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ10166.1"
                     /translation="MNYEMHKETPIYTELEKYSKEHIIHFDVPGHKKNTSSPLAEAFG
                     ERIALLDANSTKALDMLSNPTGVIKKAEELMADAYNADYAFMLVNGSTFGIISMIMSA
                     CDPKDKIIMPRNIHKSAVSGIILSGAVPVFIDPEIDIEYGITNGITYDSVKKAIKYNP
                     DTKAVFIINPTYFGTVSDLRAIIRLCHRHNIAVLVDEAHGSHFPFHPDFPDGAMALGA
                     DMSTVSIHKTGGSLTQSSALLLNERIIKKEKVRTIVNLMQTTSASYILLTSLDVARRH
                     LVLNGREIFGKLLSLCEKAKTEISKMPGLNVITRDEYVNGKEIYDYDETKIVVRVNDL
                     GLTGFEVYDLFKNEYSIQLELAETYAVLAVTGPGDNEESINYLVDAFRDLSLKYYGKR
                     QPLKIPLMDYFIRPKTVILPREAFYSRKRSVPISEAMGEISGESVMIYPPGIPLVIPG
                     ERITDKVIEHYNFYIKQDCVIMNDDGNPDFIKVLGE"
     gene            14071..14934
                     /gene="speE"
                     /locus_tag="PK733_06195"
     CDS             14071..14934
                     /gene="speE"
                     /locus_tag="PK733_06195"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOJ10167.1"
                     /translation="MIDLWYSEYHTDDVRFSIKVTQHIYTEQTRFQRIDFFRSETFGT
                     FFTLDGLMMVTQKDEFAYHDMICHPAFATNPDIKNVLIIGGGDGGTAREVSRYTTVER
                     IDMVEIDERVVRLCQKYLPQTSCVIEKDKRINLYFEDGVEFVRNAADGAYDLILVDST
                     DPVGPGEGLFSAEFYRNCYRILSTEGILVNQHESPYYKTYAHEMKRSHDKLASTFPIS
                     KVYEFYMPTYPSGHWLFGFASKLLDPINDFKPEAWEKLNLKTKYYNTELHTGSFALPT
                     FVKELLDNEED"
     gene            14953..15879
                     /gene="speB"
                     /locus_tag="PK733_06200"
     CDS             14953..15879
                     /gene="speB"
                     /locus_tag="PK733_06200"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOJ10168.1"
                     /translation="MKKNGVLNKNFLGNDIFRSNFSSNNFLSRNFLGCECDLRSSDLV
                     IFGAPFDGTTTFRPGARFAPAQMRVESIGIETYSPYFDVDLLDFNIHDAGDLDLPFGE
                     KEKVMDIIGQTAGNIFLLDKVPLMIGGEHLVSLPVIKEACKKFSELNMIHFDAHTDLR
                     EDYLGEPLSHSTVIKRVWETLGDSKIWQFGIRSGTREEFYWARKGHTYLNMHNFKGLQ
                     EAVSNIGKKPVYLSIDLDVLDPSILPGTGTPEAGGVSFLDLIEALKEVSKLNIIGADI
                     VELSPHYDHSAVSTAVACKVLRELAITVLLPT"
     gene            complement(15872..16756)
                     /locus_tag="PK733_06205"
     CDS             complement(15872..16756)
                     /locus_tag="PK733_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOJ10169.1"
                     /translation="MKKAILIYNPLSGQRSLPGKLDSIIARFMDKGILLQPYRIYSDR
                     QYELLLEVLKTENYSSAVISGGDGTINTIINTMLINNINIPVGIIPSGTCNDFARSIG
                     MPSNINQCLDIILSGKTIDIDVGLVNGERYFLNTCAGGILVDVSFNTRGDLKKNIGPL
                     AYYLTALGEISNIRPFNLKLKTEDDYIEVETLLFIILNGRHVGGFNNIAWKADLTDGL
                     MDIVIIKNCWHIDMAGILLKVLANDLMRDKNIISFKAQKCTIEGDINVALTLDGERGP
                     DLPISVEFINKRLSVFVR"
     gene            complement(16789..17751)
                     /locus_tag="PK733_06210"
     CDS             complement(16789..17751)
                     /locus_tag="PK733_06210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ10170.1"
                     /translation="MKNKVTVTGANFIFFIFAALFIFSQFVLTIIIGISSAIYGPDYA
                     NDFVSRNIYILTVINEYLFILGPVIVYAIIKKLDFKETFRLNSPGLFPSVLIVLMSVP
                     AYFVASMLNSLVIYLLQFLGNIPPQPIPVPRNINELLAGMFFIALTPGICEEMLNRGL
                     LLKAYENRGTVKAIIVSSIFFGIFHFDITNLLGPIFLGLLIGYYVVRTNSIFAGILAH
                     FLNNAIAELLQYFFVNEIETEITSISGQEVLSAILYGVVGLMVLGILALLFKKVTEGR
                     YSYKPPISSIKKDVISIITHWPILLVILLYILMAMLYLLIIIKI"
     gene            complement(17748..18386)
                     /locus_tag="PK733_06215"
     CDS             complement(17748..18386)
                     /locus_tag="PK733_06215"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOJ10171.1"
                     /translation="MNYANKSSIRKKILDIRENTVKEDIVNNSKIIIKKLVSMESYNK
                     SNVIMCYVNFRNEVITKDFIKESLLNGKRVAVPLIYKEVNKHKDTTDTTDTKSSAGTA
                     GSKKIICCEIKNMDTELEKGVLGILEPKEEYRREIKPEEIDLVVVPGTAFDANKNRMG
                     YGVGYYDNFLKNIREDCITVGVAFDFQVLDEIPVEKHDIALDMIITEKRVIV"
     gene            complement(18456..19136)
                     /locus_tag="PK733_06220"
     CDS             complement(18456..19136)
                     /locus_tag="PK733_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOJ10172.1"
                     /translation="MHIVDIIYRIYKVAGSLICHQIPSRTIYAGGIPLPLCARDTGIY
                     MGLFSSLAYIYLRRKNKSDSPPDIKNTVLLCCLMLLMIIDGGTSYLGIRNTNNIIRYF
                     SGAFFGLPLPFFLLPAAYYNPKRSNMHKSLDKTAEIILLIIINITIGYLLIEMDVLPW
                     ILPATATIASLIYIITRTVYTVVVRTQLTKAPYMQLLVAGGTLGIILIMFFISTYILQ
                     PLKDTLLR"
     gene            complement(19204..19794)
                     /locus_tag="PK733_06225"
     CDS             complement(19204..19794)
                     /locus_tag="PK733_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10173.1"
                     /translation="MVNEMRKCEYCGEDIHFKSSRCPFCGSLLKMNISNDTNSSTNIN
                     TNTNIDANADANADINADTNVDTNANAAYIQNNNVAGIDYEDRNYTGNIYNVDEYTGG
                     SQTKNSYEDNKPAPVPEYQLSNGMKVFLTVIASIIPGLGQVIGIIIAIVFMNSEEDPD
                     RRSFGVALLVSSVIIFVFACLSCFVAILAAGQFSQL"
     gene            complement(19831..21747)
                     /locus_tag="PK733_06230"
     CDS             complement(19831..21747)
                     /locus_tag="PK733_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOJ10174.1"
                     /translation="MAHTVRIHTINNNSCNNIENIENFYVENGKNLLDFLREKSLYVP
                     TPCGGKGTCGKCRVKVKGYIEEPSEKEKKLLGRNAEAMGYRLACYNKIESDIDIFLDN
                     SDKEASIVTSGIEKTIKVNPLIGKKCVKLDMPDIHDQRPDVDRIIEAYDSDSVVSSID
                     LIADIPELIRKEYTVTVVSHDGEITGVEPGNTENRLYGIAVDIGTTTIAAYLYNLGSG
                     KKLDTYSTLNPQRKYGADVLSRIEYTNNSVSSKMEMHKVIIDCVNKIVDVFAENNSIE
                     KNDIYSVVFAGNTTMIHFLMNLPAKNIALSPFIPVTTNMHKIHPSSLGININNGGLVI
                     ILPSVSAYIGADTVAAVLSSNMHKSNEIYLLIDIGTNGEIVLGNKDWMYSCSTAAGPA
                     FEGANIRNGIGGIKGAVDKIHFNNKLEIETIGSEKAIGICGSGIVDAVAGMLDKGIID
                     ETGRIIDNYIGPGVSEGEVECEGKGESKADNLEEDYKERFVKIEGLNSFLLIKSEESG
                     IDNDIVINQKDVREIQNAKAAIAAGIKVLVKQAGISWENIAKVCLAGGFGNYINTKNA
                     LKIGLLPAEIAEEKIVSIGNAAGAGAVEGLLSRSALEEMEIIKDKIKYIELSAMADFV
                     DEYVDCMFFENINL"
BASE COUNT         6824 a         4300 c         3418 g         7403 t
ORIGIN      
        1 catcatggag tttgaaataa taatccctat tggtccaata ataacatcta ccttgcggga
       61 attataaatt attgcatttt ctcctgtagc accttctttg gctcctgctt ttaacataat
      121 tgcagtagca gatgaatttg tgccaagtgt aataatattt acatcttcag taagcttttt
      181 atcagataat tccttaacta gcttttctac tatggctttc ccaatacctc cgccctgtcc
      241 gtcaattact gctattttca tttttgataa tcctccgaag cctgcttatg taatatttta
      301 taaataatgt atacgcataa tcatataaca aaaagacaaa ttgaaaaata ataacagcag
      361 cccacaatgg cagctttata ttttcactaa ccaaaaacag cttttccact aaaaatacag
      421 ccgctgccag gcataaatta aaatacaaat atttcagtat atattccagc ggtgcatttt
      481 taattctttc agtgtagtat tttataatac catataggcc gaaaaaaaca gtatatggta
      541 ttattcttat tttatccggt acaactatga atgccacaag atttgttgct atgtaaaaag
      601 cccatcctgc tgctaccttg tattccataa taataattga tacaaaaaat gaactaaatg
      661 catataaaga taattttgtt gtaggaagta ttgctgcaag tagaagacat ataacagtca
      721 atgctcccaa aattccgttt aaggcaattt ttttagtatt gctgaccatt ttaacccctc
      781 tatatccaac ttattctcaa atctgcatgc aatttatcaa cagcatggta tcagatctcc
      841 gcccatacac tcacagcagc aatctgaaca aataaggcat tggcaaaaac tgcaaatatc
      901 ggtatcatta tttgtgccat atccgctttg ttttctttga ctgtaagcat tgtatatcct
      961 gctaagagtc gctctgtatt caagatttcc agggtccata tttacggctg tttgaatatg
     1021 tgtgtaggct tcatcctgcc agccttttct caagaaaatc aagcctttta aataatacca
     1081 ttctgcattt ctgacgcttg aactatcgag tatttcctca gctgtacgta tattgccgtt
     1141 attaatatga actcttacat tgctgaagaa atcatctctg taatacccat tattgctatc
     1201 tgtattagta ttattcgtat ttttatatcc gccataggaa taatttgttt tcggtcctgt
     1261 tgttctttgc ttacttaata cttcataggc ttcattaatt tctctcaatt tttcctcggc
     1321 aagttttgcc aggggattat ccctgtattg gtcgggatga tattttttta ccaactcccg
     1381 ataagccctt ttaatttctt cttcatcggc tccctctttt atacctaata ctttatatgc
     1441 ttcatatgga ttcaaagctt ctacaactcc ctgtacctaa aattttttct gtttttctta
     1501 acataccaat ataaattata ttttcaatta ttccttttac tttttttgca tccatcagtt
     1561 cataagtttt tataacctgg tcaagtgaat aaataagtac aaactcaatt ctttccttaa
     1621 tctcattttt aatttgattt atttcctcat taattttatt tttttcactg tttttattcc
     1681 agtatttaac cagcaacgga ttatactttt tattcctggc atcttcctcc atgtcatcat
     1741 atgcatcaag tatataaatc catttcccaa ggttatagcc catccatcgc agcatctttt
     1801 ccactttctt atctgcacat aggggttcat atgccataac ttcttccatt atttttgaaa
     1861 aaggttctgc aacttcatca atagaatcac atttcctatt ctcaagttca aataattcat
     1921 tgagtttttt ttcaattata tcacattttt ctttatattt gtgctttaat tttttataat
     1981 aaggacttaa aaaaaacatc ccggttgagg aaacaaaatt gtggttgtct attttatcat
     2041 ccttcaaatt atagtaagcc aggatgatat tcatatcagc agcataatca actatttcat
     2101 tttcagctgc tatgtttttt ttctttacag ggtgcaacat gcacctttcc tgtattatac
     2161 taatgctgct atctgataaa gagaataaaa gtatggcaag gaatgtagaa tcatagttta
     2221 atgcaacacg tggtatttgt ccgattcttt taccaataga tttgcatact ccacaataaa
     2281 aagctttaaa catctcatac tcttttattt taagctctgg cttttcaggc attatataac
     2341 caaacaaaaa atctcacctt caattgtcat aatcagatga tgaatctatc aggcttcatc
     2401 actcttattg tttttctttt ttaaaaaatc tgctgcatac atgaaaaatg aaaataacat
     2461 aaaaagtaga gcaataaaaa taaatatatc atggaaaggt atattgaaaa gtatcattac
     2521 tattgcaaca tacagtatta ccgtagaaag ttttccgtac caattggcct ggacaaccac
     2581 tttatttttg ctatatagaa aaaagctccc cacaatcata gtaacctctt taacaagaat
     2641 aataattaca acagctcgag gtatcttccc ctgcatgttt aacataaaga gggctgtcaa
     2701 ctgcataagc ttgtcagctg ccgggtcagc caatttgccc catggcgtaa tcaagttata
     2761 ctttctggcg ataaaaccat ctagtatatc tgttaatcct gctatggtaa aaattataac
     2821 tgcaagtaaa tattgccttt cataaacata gtatccaaaa gcgggtacta aaaaaaatct
     2881 tattgtagtt agtagatttg gtatattcac tcggccacca cctgacatta tttaatatct
     2941 aaatctataa taaacagtac ggttttaaat aacgtatctt tacttgactt aatgtaactt
     3001 agctttgcat tgtttttatc agcaactcat aaatgtgagg aaaatgtttt tttatattta
     3061 aaggttttaa attagtatct gtccaaacat gaattgtttc cccaattgct ataggagtat
     3121 tctcattttc tctgaacact tcataattga aaataatacg actcaaggtc agcttattta
     3181 ttcttgtttt aactataatt atatcttcat atttagcagg acttttgtaa atacacttta
     3241 gttcaataag aggaagcatt ataccttctt cttctatttt agaataaggc aacccaactt
     3301 tccttataaa atcagttctc cctgcttcaa accatattgg ataatttgaa tggtggacta
     3361 tccccatcct gtctgtttca gcatatctta ctaccaatct tgtttctgac tccagcataa
     3421 ttctctccct tatacactac actaaattgc attatatttt attttatttt ataaaaaata
     3481 caatttagta taaaatataa acccgtgctt atattaaaca cgggatttaa agaaattcag
     3541 actattttcc ggtctgattt cccaattagg attatatcaa acacctttct agaaaatccc
     3601 aatataccat ccatttcaat gattgctcca caagctacag ccaatagcta tagtactaga
     3661 agcccttctc aataataacg ctatgagctt cttcaacttc agcttcaggc actaggatct
     3721 caaaataatc actactgctt tcctgatttt tgcttatagg tcttaattta actaaaatac
     3781 cttcttttgt aagcaatcct tgcagatttt ctgcaatatc tttgctttga gccatataaa
     3841 ctactgtcca cataaccatt acacgcccct ttatttttac tctcccgaat gaatttcccc
     3901 gtggcggcaa taaataattg ctttaccccg tattttaatt gctgaagtgt tttcagtaaa
     3961 ctgagccagg agaacctcgc ctttatccag cttttctgta tgatgaaact tcgtgtcttt
     4021 tccgcgtgta agaccaatta tatttacccc attttcaagg gcttttatta ctatatattc
     4081 ccctatcgca tctatttgtt cattatccaa aactaccacc tcgacataac tatatattat
     4141 ttcgtgttaa aaatcaatat aaaatttcca attacggtca aactaccttt atgttgccgt
     4201 ttcccattct ttcttttaat tctgataaaa gtgtttcatt aaggttgatc ccgtattccg
     4261 tgtccacaat ttcgcttatt ccttcatttc tatagtaaaa gtgcacagga gttttcccct
     4321 tgaaaaattt gagcagggac agaagtgatc tgttattgct gctataatta ttcggtctct
     4381 cgtcaaaaat aatgtaaact cctgatttat ttcccacgga aatatcatct cctgtcagag
     4441 gctttacttc ctcacaaata atctttggtt cttcctcttc ttttatactg actctgcctt
     4501 tgacaagcac aatgttttct tcgaggatta tgtcctgata catagatagg gtttgtggga
     4561 aaataattac ttcaatcctc ccatatagat cttcaagagt aacaaaggcc atcaggttat
     4621 catttttcgt agcttttatt tttttctcgg aaattatccc tcctactata acttttttcc
     4681 catcttctaa aaccccatca ttgctattat caaactccag ttcatctata gactcaccgg
     4741 ctttttcctt aaaatcaaga ctgtttgctg tggcatgtat agataataaa tccttgaact
     4801 cctcaagggg atgccctgaa acgtaaaggc ccagcatttc tttctccatt gccagaagca
     4861 cattgggcgg atattcattt atatccggat aagcaatttc agtttcatat atatcgggag
     4921 agtccttctt ttgaggaata tcaaaaatag acagctggcc ttcaagattt ttccgtctta
     4981 tttcctgtat actgtcaatt actttttcaa ataccaaaat aagtttggat ctatatattc
     5041 ccatagaatc aaaagcaccg cattttataa ggctttccac acacttctta ttaacatccc
     5101 tgaagcttac tctctcacaa aaatctctaa agctaatgta ctcaccattt ttttgccttt
     5161 catttattat ttccttaaca gcattttcac caacattctt tacagcagcc attccaaacc
     5221 ttattttgcc atttactaca ctgaatttaa tatcactttc atttatatcc ggtgataaaa
     5281 cctcaatacc catagttttg cattcgttga catatctgga tattttgtcg gtacttccaa
     5341 gaaaactgtt taataatgca gccattagtt cagttgggta ataatactta agccatgctg
     5401 tctgatacgc tatcacagca taggcagcag catgggattt attaaaagca tagcttgcaa
     5461 aatccatcat ctcgttaaat attttatttg ctgttttttc atcagcacca tttctaatag
     5521 caccctttat aagtacattg ccattttcat cagtcctgcc atatataaag ttctgccgct
     5581 cctctttcat tatgtttgct tttttctttg ccattgctct tcttacaagg tcagctctac
     5641 ccattgaata acccgcaagg gtacgcacaa tttgcataac ctgttcctga taaaccatgc
     5701 atccataagt tacattaagg atgttttcca gcaaaggatg gtcgtatttt accatttccg
     5761 ggttaatttt attgcttata taccggggta tttgatccat tggacctggt ctgtaaagag
     5821 aaataccagc tattatatcc tccagagatg atggcttaag ttccttcata aactgtgtca
     5881 tccctgcgct ttcaagttgg aaaatacctg ttgtccttcc ctcgctaatc atcttatata
     5941 ctttagggtc gtccatttcc atgttatcgt aattcatgat tatgccatga ttttttttaa
     6001 ttaattctgc tgcatcacgt aatactgtaa gagttcttag gccgagaaaa tccattttta
     6061 gcattccaag ttcttctaga tgtcccatgg taaattgagt agtgatactt tcatcattct
     6121 tttgaagagg tacatattca accacaggct cttttgatat tacaacacct gcagcatgcg
     6181 ttgatgagtg gcggggcatt ccttcaaata accttgatgt atctattaaa tcttttattt
     6241 tttcatcttt attataaagt tccatcagat cgtggttaat ttccagagct ttatctatgg
     6301 tcatacccaa tttaaaaggt atcatcttag caatcatatc aacctctcca taaggtatat
     6361 caagagctct tcctacatct cgtattacag cccgggcagc cattgttcca aaggttatta
     6421 tttgtgatac tctattggct ccatattttt ctataacata atctataact tcctgcctgc
     6481 gttcataaca aaagtccaca tcaaaatcag gcatacttat tcgttcagga tttaaaaatc
     6541 tttcaaacaa cagattgtat tttagcgggt caatattggt aattccaaga caataagcag
     6601 caatgcttcc tgcaccggaa cctcttcctg gacctaccat aatcccttta tcccttgcaa
     6661 attttataaa atcccagact atcagaaaat aatctacata acccatgttc ttaattactg
     6721 aaagctcata gtccagacgt tcttttttat cattgccgca atttgggcca tatcttctgg
     6781 ttagtccttc aatacaaagc ttttcaagat attcaaaatg atcctggttt cccggtacat
     6841 catattcagg taagtgaagc acattgaact ctaattccac attgcagcgc ttcgcgattt
     6901 ttactgtgtt ttcaagagca tcaggaatat ttttaaacag tgcagacatt tcctcgggag
     6961 atttaagata tacttcttca gtattaaatt tcattctatc ttcgtcgttt attcttttcc
     7021 ctgtctgtat gcatataagg atatcatgag ctctagcatc ttctctcttc aggtaatgga
     7081 catcattagt agccaccagt ggtatttcgg tttctctgct tagctttatt aagttctgat
     7141 ttacaagatt ctggttttca atgttgttaa cctgaagttc aagataaaag ttatttctgc
     7201 cgaatatttt atcatattcc agtgctagag actttgccct gtcataattc ccctgtagaa
     7261 ttgcagtagg tatatctcct gaaagacacg cactgagagc aattattcct tcattgtatt
     7321 tctttaaaac ttccatatca accctgggtt tataatagta gccttcaata aaacctgacg
     7381 acactatttt catcagattt ttatatccca ggttattttc agcaagaagg acaagatgac
     7441 cttgttctga atcaactccc gattctttgt caaacctgct tcttcgcgct gtatatactt
     7501 cacacccaag aatgggtttt atattgtttt ctactgcctc tttataaaaa tcaatgatac
     7561 catacataac cccatggtcg gttatagcaa cgctttccat gcccagttcc ttaactttgg
     7621 ctattaaatc tttaatcctc actgctccat caaggagact gtattcagta tgaatatgca
     7681 aatgaacaaa tttatttaaa ttattcaaag taatctacct ccattatatg gaacaacatt
     7741 tattaagtat atcaagaacc ttcccattta ggtccttaag cttttctgca gcatgggttt
     7801 tatcattgcc aaacacttca atgtatatct tgattttcgg ttcagtacct gaaggcctta
     7861 tgcaaaacca ggattcatca tccagttcat aataaagaac attggattca ggcaaaccaa
     7921 ggtcttccct ttcaccggac tttaaaacat atctttcgcc ggtaaggtaa tcccttatgg
     7981 catatatttc atacccatct aatttaaggt tactatcttt tctcaacttt tccataatac
     8041 tttgaatttg ttccaaacct tctttacctt ccagcgtaaa agaaattact tctacatcag
     8101 taaacccata attcccatat atttcttcaa agacctccat taaagtcttc cctaatgatt
     8161 tataataggc cgccatttct gcaataatca tagcagcaga tacagcatct ttatctctgg
     8221 catatgttcc agctaaatag ccatagcttt cttcaaagcc aaggaggtac tttttgtctc
     8281 cattttcgtc cagaagcctt attttttcac ctataaactt aaatcctgta agaacctcta
     8341 ctaccttaat atgatatttc ttagcaatag ctttagctaa ttcagtcgtt actattgttt
     8401 ttacaataaa tccatttgaa ggcaaactac cttttaaact tttttgcgaa agtatatatt
     8461 ccagcataag acagccgatt tgattccccg taaaggatgt atagctgcca ttattatctt
     8521 ttgccattat acctactctg tcactatcag gatctgttcc aataataaga tctgcatcat
     8581 ttcttttggc catttcaagt gcgattttaa atacactttc atcctcaggg tttggagttt
     8641 ttactgttgg aaaatccgga tttggctcct cctgttcttt aacaacaaaa acgtttttaa
     8701 agcctatttc atttaatatc ctgcgtacag gtttattccc cgctccatta agtggtgtat
     8761 atataatttt aaatgtatca ctttgcttgc atattatatc aggatttaag caataagttt
     8821 ttaagacatc gatataccga tcgtcaattt ctttccctat tatctgtagt aatccagtct
     8881 gtaatgcttt tccctgttcc ataatcttta cctgagaaaa atccttaatc atatctatct
     8941 catttagcaa taagtccgac tcatcaggca gcagctgtgc cccatcttcc ccataaacct
     9001 tatatccatt atactctttt gggttgtggc ttgcagttat tgctatacca gcagcagcct
     9061 tcagatacct tattgtaaag gaaagtacag ggacaggtct taattcatca aacaaccagg
     9121 ctccaatgcc gttgcctgca aagaccaatg ctgcttccat ggcaaattcc ggagatttat
     9181 gccgggaatc atatgcaatt acaattcccc tagctgccgc attactgcct tttttattta
     9241 tatagttagc gataccctgc gaaacttttc ttacaatata tttgttaatt ctattggttc
     9301 ctgcaccaat aataccccgc aatcctgcag taccgaactc aagatccctg taaaacctct
     9361 cctcgatctc ctccatgtta tcttttattg atgcaagttc ttccttcgtt acatcatcaa
     9421 agtaatcatt ctcaagccag aacaagtatt tgtcataagc tttcataaga atcctccaat
     9481 cttatttagt gccaggcacc ataactgact tttgttcaag tccaatcttt cacttctcac
     9541 ttctagttgg gtgcatttaa catatttgaa taaattattg gtaagtggca ttaatggaaa
     9601 tgctggcaaa tattccttca aattttataa gtacacccct cctatttata tattttacct
     9661 taaaaaaaga aaaaaaacat cttattaata tgtttttagt aatattagtc caatatacgg
     9721 acatatatta atattgataa taatggtgca agtactattt tcgtaacagt ttggcaaaag
     9781 gcaaattaaa tagggggggt ctttggtgga aagttataat atatacaggc aaatagcaga
     9841 aaggaccaag ggggatattt acataggagt cgtaggtcct gtacgtacag gtaagtcaac
     9901 atttattaaa cgctttatgg atttattggt tataccgaat atagaaaatg aatatgacag
     9961 ggaaagggca aaagatgaac tgcctcaaag tgcaaccggc aggacaataa tgacaactga
    10021 gcccaaattt gtccctaacg aagcaataaa gatagtattg gatgaaaatg cccaattaag
    10081 ggtcagattg gttgattgcg tgggatatct ggttaaagga gcgcttggtt atctggaaaa
    10141 taatactccg aggatggtat caactccatg gttcgaccat caaattcctt tcgaggaagc
    10201 cgctgaactg ggaaccagaa aagttattaa tgaacattct acgatcgggc tggttgttat
    10261 tacagatgga agcataactg aaatacagag agatgaatat ttaggtgcgg aaaagcgtgt
    10321 gataagcgag cttaaagcta ttaataaacc atttatcatc atattaaact ctacaagacc
    10381 ctttgctggt gaaacaacca aactcaagga ggagttggaa gaaaaatatg gtgtacctgt
    10441 tgtaaatgtt aatgtagctc agatgagaat tgaggatgtt aataatatta tggaaaagac
    10501 actttttgag tttccgatta ccgaggtagg tataaatact ccgaaatgga ttgatgccct
    10561 tgaaaatgat cattggttga gagttaagct acttgattct attagagaag cttttaaggg
    10621 aataagaaaa ataagagaag taaaaaatgc tgtctcgaag ttcaacgggc ttgaatttat
    10681 aaaaaaagca tatgttgaag aaataaacct gggtaccgga agtgtaaata tagagctttc
    10741 tcctcccgat ggccttttct atcgcatact gagtgaactg actggtcttg agattgaagg
    10801 cgaccacaga ctcattgggc tgatgaaaga tctggcaaga attaaaaaag aatatgaaag
    10861 aatcgaatat gcactgcatg aggttaaggt taaaggctat ggcatagtta tgccacaggt
    10921 ggaagaccta tctctggaag aacccgaaat cataagacag ggcagtaggt ttggtgttaa
    10981 actacgtgca agtgccccca gtgtgcacat gatccgcgca gatattgaaa ctgaaattgc
    11041 acctctggtt ggcagtgaaa agcagtcaga agaattggta gactaccttc ttaaagagtt
    11101 tgaaaatgac ccgggtaaaa tatgggattc taatatcttc ggcaaatcac tccatgacct
    11161 tgtaagtgaa ggcctgtata ataagctgag cagaatgccg gaagattccc agttaaagct
    11221 tcaggaaacc ttgcagagaa ttatcaatga gggaagcgga gggcttattt gcattatttt
    11281 gtgagaaaca aggggacggt tctgttgttt cgttcccctg tttaaaagta ggatacaatg
    11341 ggacaatggg gacggttctc cgaaacagga gaaccgtccc cattgtccca tttgtaagcc
    11401 cagcaacagc aatataaagc attttgcatt aaatcttaat taaatgttat aataggtatt
    11461 gtaatttgaa ataatcatgc taaaatataa attattctgc ttaatcaaat aatctggcga
    11521 aattggaggt tgataatgta aaaatgaaaa gaactgaacc aaataaaata aaattgcatg
    11581 gatttaataa tcttacaaaa actttatctt ttaatatgta tgatatctgt tacaccagat
    11641 cggtatcgga caggaatgct tatatttcat atattgatga acaatataac gctgaaaggc
    11701 ttacaagcat attaaaacat gtaactgaaa ttataggtgc caacatacta aatattgcca
    11761 accaggatta tgatcctcag ggagccagtg ttactttgct tgtatgtgaa gaacctattg
    11821 gcactgtttc aaatcaaaat aatgcaaatc aaaataatac gaatattcgc ggtaatactg
    11881 ataatgataa tgcttcaaat agtaataaca ctgataatat tagacgagag gcagtaatag
    11941 ctcatcttga taaaagccat attaccgtac acacttaccc cgaataccat ccagatggtg
    12001 gtgtttgcac cttcagggca gatattgatg tatcaacctg cgggaaaata tctcctctca
    12061 atgcactaaa ctatcttatt aatagtttcg aagccgatat tatgtcaatt gactaccgtg
    12121 taagaggttt tacacgtgac attaccggca gaaaaatttt taacgaccat aaatttaatt
    12181 ccatacaaaa gtacatttca aatgaagtga ttaaccacta tcatatgatt gatgtaaatg
    12241 tctaccaaga aaatattttt cataccaagt gcaaacttaa ggattttaac cttgataatt
    12301 atcttttcgg tttctctgaa aaagatattc atccgggtga agcaaaagaa ataacccatc
    12361 gtttgcagaa ggaaatggat gaaattttct atggacaaaa tttgtattaa tatttattaa
    12421 taagtattaa tacatagatg aatagtaata ttatacaaag catgtgcttt tattatattg
    12481 ctataatata actatgagct attgctgtaa tcatacaagg gcgaattcat ttcgcgaaca
    12541 gtaggaatgg agcttaaaat aatgaattac gaaatgcaca aggaaactcc aatatacaca
    12601 gagcttgaaa agtattcaaa ggaacatatt atacattttg atgtgccagg tcataagaaa
    12661 aacacatcct ctcccttggc tgaagcattt ggagaacgga ttgcactgct tgatgccaat
    12721 tctaccaagg ctcttgatat gctttcaaat ccaacagggg ttatcaaaaa agctgaagag
    12781 ctaatggcag atgcttataa tgctgactat gcatttatgc tggtaaacgg atctactttc
    12841 ggaatcattt ccatgattat gtcggcatgt gacccaaagg acaaaattat aatgccccgc
    12901 aatattcata aatctgcagt aagcggtatt attttaagcg gtgctgttcc cgtatttata
    12961 gatccggaga ttgatattga gtatggcata acaaatggca tcacctatga tagtgtcaaa
    13021 aaagctataa aatataaccc tgatactaaa gctgttttca taataaaccc tacctacttt
    13081 ggtactgtat cggatttgag agctataatc agactttgcc acagacataa tattgcagtt
    13141 ctggttgatg aagctcatgg atctcacttt ccattccacc cggatttccc tgatggagca
    13201 atggctcttg gtgcagatat gtctactgta agtattcata aaaccggagg gtctcttacc
    13261 cagtcatctg ctctgctttt aaacgaaaga ataatcaaaa aggaaaaagt aagaaccatt
    13321 gttaacctta tgcagactac aagtgcttcg tatatactat tgacaagtct tgatgttgcc
    13381 agaaggcatc ttgtgttaaa cggcagagaa attttcggga agcttttatc cctctgtgaa
    13441 aaggctaaaa ctgaaatatc taaaatgcca gggcttaatg ttataacaag agatgagtat
    13501 gtaaacggta aggaaatata tgattatgat gaaactaaaa ttgttgtaag agtaaatgac
    13561 cttggtctaa caggctttga ggtatatgat ttatttaaga atgagtatag tattcagctt
    13621 gagcttgctg agacttatgc tgtacttgca gttacaggcc caggcgataa tgaagaaagt
    13681 ataaattacc tggttgacgc atttagagac ttaagcttga aatattatgg aaagcgtcag
    13741 cctttaaaaa tacctctaat ggactatttt ataaggccaa aaactgtaat attaccccgt
    13801 gaagcctttt acagcagaaa aagatctgta cccatcagtg aagcaatggg cgaaataagc
    13861 ggcgagtcgg taatgatata tccgccgggc ataccacttg tcattcccgg cgaaagaata
    13921 acagataaag ttatagagca ttataatttc tatattaaac aggattgcgt aatcatgaat
    13981 gatgatggaa atccggattt tattaaagtt cttggagaat aatgtatagt tgccggacaa
    14041 ggcagtacta aaatagtaaa ggagaccgtt atgattgatt tatggtattc agaatatcat
    14101 acagatgatg taagattttc aataaaagta acacaacata tttatactga acaaaccagg
    14161 tttcagagaa tagatttttt caggagtgaa acatttggca cttttttcac ccttgacggc
    14221 cttatgatgg ttacccagaa agatgaattt gcatatcatg atatgatatg ccatcctgct
    14281 tttgctacaa atcctgatat taaaaatgta ttgattatag gcggcggcga tggaggtaca
    14341 gcccgtgaag tgtcaagata cactactgtt gaaaggattg atatggttga aattgatgag
    14401 cgtgtagtaa ggttgtgcca gaaatacttg cctcaaactt cctgtgtcat tgaaaaggac
    14461 aagcgtatca atctttattt tgaagatggt gtagaatttg taagaaatgc tgcggacggg
    14521 gcttatgacc ttatacttgt agactctaca gatcccgttg gacctggtga ggggcttttt
    14581 tcagctgaat tttaccggaa ctgctatagg attttgagta ctgaaggtat tctggtaaat
    14641 caacatgaaa gtccatatta caaaacatat gcccatgaga tgaaaaggtc acatgataaa
    14701 ttagcaagca ccttccccat atcgaaagtt tatgaattct atatgcctac ttatccatca
    14761 ggccactggc tttttggctt cgcgtcaaag ttacttgacc caataaatga tttcaagcct
    14821 gaagcttggg aaaaactcaa tttaaaaaca aagtattaca atactgaact gcacacgggc
    14881 tcttttgcct taccaacttt tgtgaaggag ctgctagaca atgaagaaga ttgatagtga
    14941 aggagtttat caatgaagaa aaatggtgtt ttaaacaaaa attttctagg caatgatatc
    15001 tttagaagta acttttcgag taataacttc ttaagcagga actttttggg atgtgaatgc
    15061 gatttacgat cttctgacct ggttattttc ggagcaccct ttgacggaac cacaaccttc
    15121 cgtccgggtg cccgctttgc accagctcag atgagggtgg aatccatagg cattgaaaca
    15181 tacagtcctt attttgatgt agatttacta gatttcaata tacatgatgc tggtgacctt
    15241 gacctgccct tcggtgaaaa ggaaaaagtg atggatatca ttgggcaaac agctgggaac
    15301 atttttttac ttgataaagt gcccctgatg attggtgggg aacatttggt atccttacca
    15361 gttattaaag aggcatgtaa aaaattttca gagcttaata tgattcattt tgatgctcat
    15421 acggatttgc gagaagatta ccttggggaa cctctctccc actctacagt cattaaacga
    15481 gtatgggaaa ctttgggtga cagtaaaata tggcagttcg gtatcaggag tggtacaaga
    15541 gaagaatttt attgggccag gaaaggacat acatacctta acatgcataa ttttaaaggt
    15601 cttcaagagg ctgtatccaa tataggtaaa aagccggtat acctatcaat tgatctggat
    15661 gtgcttgatc cttcgatttt accaggtaca ggcacaccag aggcaggagg tgtatccttt
    15721 cttgatttaa ttgaagcact taaggaagtt tcaaagctga atataatcgg agctgatatt
    15781 gttgagctgt caccacatta tgaccacagc gctgtatcaa ctgccgttgc atgcaaggtt
    15841 ttacgggaac ttgccataac tgttctgctc cctacctaac aaaaacactc aggcgcttgt
    15901 tgataaactc tacagatatg ggaagatccg gccctctttc tccatcaagc gtcagagcca
    15961 cattaatatc tccttcaatt gtacattttt gagccttaaa gcttatgatg tttttatcac
    16021 gcattagatc atttgcaagc accttaagta gaataccggc catatcaatg tgccagcagt
    16081 ttttgattat tacgatatcc ataagaccat cagtaagatc agccttccat gcaatattat
    16141 tgaagcctcc tacatgtctt ccatttaaaa taataaataa taatgtttca acttcaatat
    16201 aatcatcttc agtttttaat tttaaattaa atggtcttat attgcttatc tctccaaggg
    16261 ctgttaaata atatgccaaa ggacctatat tttttttaag atcacctctt gtattgaaag
    16321 aaacatcaac taatattcct cctgcgcatg tgttaagaaa atatctttca ccatttacca
    16381 accctacatc aatgtcaatt gtctttcctg aaagaataat atcaaggcac tgatttatat
    16441 tggaaggcat acctatactt cttgcgaaat cattgcatgt gcctgagggt attattccaa
    16501 caggtatatt aatattatta atcagcatag tattaattat agtattgatt gtcccatctc
    16561 cacctgaaat aactgcactt gagtagtttt cagttttaag aacctcaagc agcaattcat
    16621 attgcctatc gctataaatt ctatatggct gcagaagtat gcctttatcc ataaaccggg
    16681 ctataatgct atcgagtttt ccaggcaagc ttctttgccc tgataatggg ttgtaaatta
    16741 atattgcctt tttcaaagcc ctctcctcca atcataaagt acccttggtt aaatttttat
    16801 tattattaat aagtataaca tagccattaa aatataaaga agaattacca gcaaaatggg
    16861 ccaatgagta attatggata taacatcctt ttttatgctg ctgattggcg gtttataaga
    16921 ataacgtcct tcagtaactt ttttaaataa aagagccagt atacctaaga ccattaaacc
    16981 aactacacca tataatattg cagataacac ttcctgacct gatatagatg ttatctcagt
    17041 ttctatctca tttacaaaaa aatattgcaa aagctcggca atagcattat tcaaaaaatg
    17101 cgctaatatt ccggcaaata ttgaatttgt gcgtacaaca taatatccta tgagtaaacc
    17161 gagaaagatt ggtccaagca ggttagtgat atcaaaatga aatatcccaa aaaatattga
    17221 agaaactatg atagctttta cagtacccct gttttcatag gctttcagga gcaatcccct
    17281 attgagcatt tcttcacaaa tacccggagt cagtgctatg aaaaacatgc ctgccagaag
    17341 ttcatttatg ttcctgggta caggtatggg ttgggggggt atgttaccta aaaattgcag
    17401 caagtaaata accagactat ttagcattga agctacaaaa taggcaggta cagacattaa
    17461 cacgattaat acggaaggaa acagtccggg gctattcagc ctgaatgttt ccttgaaatc
    17521 taatttcttt ataattgcat atactataac aggaccaagt ataaataagt attcatttat
    17581 cactgttaaa atgtaaatat tccgactaac gaaatcattt gcataatctg gtccatatat
    17641 agcacttgag ataccaataa ttattgtcaa aacaaactgt gaaaagataa acaatgcagc
    17701 aaaaatgaaa aatataaagt ttgcacctgt aacagtaacc ttattcttca cacaatcact
    17761 ctcttttctg taattatcat atcaagtgct atgtcatgct tttcaacagg tatttcatcc
    17821 aatacctgaa aatcaaaagc aacaccaaca gttatacagt cctctctgat attcttcaga
    17881 aagttgtcat aataaccgac accatagccc atcctgttct tattagcatc aaaagcagta
    17941 cccggtacta caaccaggtc aatttcttcc ggtttaatct ctcttcggta ttcttcttta
    18001 ggttccagaa tccccagaac tcccttttct aattcagtgt ccatattttt tatttcgcag
    18061 catataattt tctttgaacc tgctgtacct gctgaacttt ttgtatctgt tgtatctgtc
    18121 gtatctttat gtttgtttac ttccttgtat attaatggta cggcaactct tttgccattt
    18181 aaaagacttt cttttataaa atcctttgta ataacttcat ttctgaaatt tacatagcac
    18241 attatcacat tgcttttatt atagctttcc atactcacta attttttaat gataatttta
    18301 ctattattaa ctatatcttc ttttactgta ttctccctta tatccaatat tttctttctt
    18361 atagaactct tatttgcgta attcatttgt cctccctctc gtactttctt tcaacactat
    18421 tgcctgctat ctgccttcct tatatgactg tatcctcacc ttaagagagt atctttcaaa
    18481 ggctgtaata tataagttga aataaaaaac ataattagta taattccaag tgtgccccct
    18541 gctactaata attgcatata cggagccttc gttagttgtg tgcggacaac aactgtatat
    18601 acagttcgtg taataatata aattaatgaa gctatagttg cagttgcagg taatatccaa
    18661 ggcaatacat ccatttctat taataaatac cctatagtaa tatttattat tatcaatagt
    18721 ataatttcag ctgttttatc aagtgattta tgcatattgc ttcttttcgg gttgtaatat
    18781 gctgccggta ataaaaaaaa cggcagaggc aatccaaaaa aagctccact aaaatatctt
    18841 attatattat tagtattcct gattcctaaa tatgatgtcc caccatcaat aatcattagc
    18901 agcattaagc agcataaaag tacagtgttt tttatatctg gaggagaatc cgatttattt
    18961 tttcttctta aataaatata agcaagtgaa gaaaacaatc ccatatatat tcctgtatcc
    19021 cttgcacata aaggaagtgg tattcctcct gcatatattg ttctggaagg aatctggtgg
    19081 cagattaaag aacctgccac cttataaatt ctgtaaatta tatccacaat atgcacattc
    19141 aatctcccat gtttccaagt cattcgttcc aactatcttt cagccaactg cctttcaatc
    19201 caactacaac tggctgaact gtcctgctgc cagtattgct acaaagcatg acaagcaggc
    19261 aaatacaaat ataataactg atgatacaag aagagctact ccaaaagaac gtctatccgg
    19321 gtcctcttct gaattcataa atactattgc aattattatt ccaattacct gtccaaggcc
    19381 gggtattatt gaagcaataa ctgtcagaaa taccttcatt ccgttgctaa gctgatattc
    19441 aggtaccgga gcaggtttat tatcttcata actgtttttg gtctggctgc caccggtata
    19501 ttcatcaaca ttgtatatat taccagtata atttctatct tcataatcaa tcccggcaac
    19561 attattattt tgaatataag cagcattagc attagtatct acattagtat ctgcgttaat
    19621 atctgcatta gcatctgcat tagcatcgat attagtatta gtattaatat tagtactgga
    19681 attagtatcg ttactaatat tcattttcag aagactgccg caaaaaggac atctgcttga
    19741 cttaaaatgt atatcctcac cacaatattc acactttctc atttcattaa ccaaatttat
    19801 tcctcccctg acttttctct taatacaaat ttataaatta atattctcaa aaaacataca
    19861 atcaacatat tcatctacaa agtcagccat tgcagatagt tcaatatatt ttattttatc
    19921 tttaataatc tccatttcct caagagcact tctggataac aatccttcta cagcacctgc
    19981 accagctgca tttccaatgg acacaatctt ctcttctgct atctctgcag ggagcaatcc
    20041 aatttttaaa gcatttttag tgttaatata attgccaaaa cctcctgcaa gacatacttt
    20101 cgctatattt tcccagctta taccagcctg ttttaccagc acttttatcc ctgcagctat
    20161 agcggcttta gcattctgga tttctcttac atccttctga ttaatcacta tatcattatc
    20221 tatcccgctt tcctcacttt ttatcagtag gaaagaattt aacccttcta ttttaacaaa
    20281 acgctcctta tagtcctctt ccaaattatc cgctttactc tcacccttac cctcacattc
    20341 aacttcaccc tcactcacgc ctggacctat ataattatca ataattcttc cggtttcatc
    20401 aataataccc ttatcaagca ttcctgctac tgcatcaaca ataccagagc cacaaattcc
    20461 tattgctttt tcagacccta ttgtctcaat ctccaattta ttattaaaat gtattttatc
    20521 aactgctcct ttaattccgc caatgccatt tcggatattt gctccttcaa aagccggtcc
    20581 tgcagctgtt gagcatgaat acatccaatc tttgtttcct agtacaattt ccccattagt
    20641 acctatatct attaataaat atatttcatt agacttatgc atattacttg ataatacagc
    20701 cgcaacagta tcagcaccga tatatgctga aacagaggga agaataatta ctaagccgcc
    20761 gttattaata ttaatgccga ggctggaagg atggatttta tgcatatttg tagttactgg
    20821 tataaatggt gataaagcaa tgttttttgc aggtaaattc atcaggaagt gaatcatggt
    20881 agtattgccg gcgaatacaa cagaatatat atcatttttt tcgatactat tattttcagc
    20941 aaatacatct acaattttat taacgcaatc gataatcacc ttatgcattt ccattttaga
    21001 tgacactgaa ttatttgtat attctattct tgacagtaca tccgctccat attttctttg
    21061 agggttcaaa gtagagtatg tatccagttt tttaccactt cccaggttgt agagatatgc
    21121 agctattgtc gttgttccta tatcaactgc aataccataa agcctattct cagtattacc
    21181 cggttcaact cctgtgattt ctccatcatg agatactact gtaactgtat attccttcct
    21241 tattaattca ggaatgtcag ctattaaatc tattgaactt actacggaat cggaatcata
    21301 tgcttcaata attctatcaa catccggtct ttgatcatga atatccggca tatcaagctt
    21361 tacgcacttt ttcccaatca aggggttaac cttgattgtt ttttctatac ctgatgtaac
    21421 aatgctagct tctttgtcac tgttgtccag aaaaatatca atatcagact ctattttatt
    21481 gtagcaagca agcctataac ccatagcttc tgcattcctg ccaagaagtt tcttttcttt
    21541 ttccgatggc tcttcaatat atcctttaac tttaactctg catttgccgc aagtaccttt
    21601 cccaccacag ggggtgggca catataaaga tttttcccgg agaaaatcca aaaggttttt
    21661 accgttttca acataaaaat tttctatatt ttctatatta ttgcaggaat tattatttat
    21721 agtatgtatc ctcacagtat gcgccataaa atctatactc ctcttatcaa actttcttct
    21781 atcaacttgt tttgttaagc agttttccgg caacttatta tttacattta ttcttatagt
    21841 agtattatca attaacaagc attagttgtc aatgactaat taagggttaa ggggtgcatt
    21901 ttctatattt gagggaaaat atggcaagaa acgatttcca tgtat
//
LOCUS       DAOSDD010000048        21935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1035_length_21935_cov_4.733272, whole genome shotgun sequence.
ACCESSION   DAOSDD010000048 DAOSDD010000000
VERSION     DAOSDD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21935
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1035_length_21935_cov_4.733272"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PK733_06235"
     CDS             complement(<1..670)
                     /locus_tag="PK733_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOJ10175.1"
                     /translation="MEAIKFRHEIKHYINMMDYYIMRNRFKAVMQADIHGDVNNEYII
                     RSLYFDNFYDSALYEKISGVNNREKFRIRFYNGDTSLIRLEKKQKCNNLTAKFSAVVS
                     RTQCEKLLIGDIGWMRESEVSLVTEFYVKMQSGLLKPKTIVDYVREAYTYPAGNVRIT
                     FDKSVRTGLFSTKFFDNNFPAIETLDKGLIILEVKFDGFLPEVISDVLQTNERRQTSV
                     LKYAL"
     gene            960..1655
                     /locus_tag="PK733_06240"
     CDS             960..1655
                     /locus_tag="PK733_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10176.1"
                     /translation="MDKKLIYIADDEVNICNIIKSFLIKEGYEVEAFNEGGSLLDTFN
                     KRHADMLIIDIMMPGIDGFSLCTSIRQKSNVPIIIVSAKDTEPDKIAGLSLGSDDYLT
                     KPFSPLELIARVKSIFRRIELDKAYAGNNESIRISDILIDPNNKQVEFGGKNIGLTRM
                     EFNLLYYLAENTNRAVGRSELLDKVWGFENEVETRATDDMVKRIRKKLSDAGSVLKID
                     TVWGFGFKIENKE"
     gene            1730..3232
                     /locus_tag="PK733_06245"
     CDS             1730..3232
                     /locus_tag="PK733_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ10177.1"
                     /translation="MKNNTIKWRIFKYNLIVIVLLITLTTIIFNVAVRLYIEKDIISQ
                     LHKIASRTEGTVLEKGPDFFPKSRGMQPPPPPDMQYNNPPDRVSEYFNFQDDNELFRF
                     YFMLDRSLREPLSVLNADYILLDNGKNIINTPPMEYFNASDDLMNKITNELSKLKDLT
                     IETYMSFHFSGTEYVAIVKPVTNKNTFGLGWIVIYSSLQKVNQLQWVINLILFAILIL
                     SAIVIIIFSSLAAKKISAPFSSLNQHIRAIAERKFSSKISMPVDDELQELVNNINVMS
                     EKLETYDKAQKTFLQNVSHEFRTPLMSIQSYAEGIKYDVVGSSSAVDIIIDETKRMTH
                     LVEDLLYLSRLDAIEESYHFSNLDLDDFMNSCIERINGIAIKGNIRITVNNLMEKVEL
                     YADEEKLSRAITNIISNCIRYAKNTVAITSKIIDVNKIELSISDDGPGFDPNELSNIF
                     ERFYRGKKGIFGLGLAISKNVIEKHKGEITAANSESGAIFIIQLPILSKQ"
     gene            complement(3514..4794)
                     /locus_tag="PK733_06250"
     CDS             complement(3514..4794)
                     /locus_tag="PK733_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ10178.1"
                     /translation="MKFSILKEKITPEFPVFQQGFSGRARKKSIGVNDDIYATVALIQ
                     SNKTVAIIAMDLLYGNRRVADEIKKAINEKYGLVQDEIIINYAHTHSVISIKGEDDDN
                     PENVIYYNTVKTKIMEAVDKAFDNLTEGDAYICKGKSKFGVSRRYPSEQGALWKPYFN
                     ENAIDKDLFLIKLVDKSNEIRGLIYNYACHPTTLGPDNYWISADYPGMVRKYLEEKYE
                     GMIPMFLQGCGADIKPYITADNGRFRSCTLDEVDQAGKSLAGEIQELIDNAGWKKINV
                     ELQTESSEVKLYSEIWSIEKWETIANNPDEPEYRKIAAKKVIDEMKNGKIKSYIPYYI
                     SLLRLDDKTCIIGLENEVVSEIGKKIKKILSDEDAIVLGYSNSIMCYIPTKDVLLNGG
                     YESASFISARLAGQFINETEDIIIGRAVVMAKHN"
     gene            complement(5084..5674)
                     /locus_tag="PK733_06255"
     CDS             complement(5084..5674)
                     /locus_tag="PK733_06255"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOJ10179.1"
                     /translation="MAKQVWKPSTILNPVPAVMVTCMGKDGKPNIITLAWVGTINSNP
                     PMVSISVRKERYSYQLLEETGQFVVNLTTEKLAFATDFCGVKSGRNVDKFAAANLTPE
                     KASRVDVPLIGESPVNIECIVRNAVELGSHVMFIGEIVAVDVDEALIDEYGKLCLEKA
                     GLICYSHGEYWSLKKSIGYFGFSVTKRKNIKRNNKR"
     gene            complement(5935..6111)
                     /locus_tag="PK733_06260"
     CDS             complement(5935..6111)
                     /locus_tag="PK733_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOJ10180.1"
                     /translation="MTTKKMEHANPGIKCVVNTCHYYLSGDHCAAERIEVQPRNAADS
                     QETDCVTFIPGSKM"
     gene            complement(6256..6789)
                     /gene="thpR"
                     /locus_tag="PK733_06265"
     CDS             complement(6256..6789)
                     /gene="thpR"
                     /locus_tag="PK733_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOJ10181.1"
                     /translation="MRLFIAINFSDDIKKRLYNVIQSLQAQSMQGRMTKQENLHLTLV
                     FLGEVETGRINKVKEVMNSIQKNKFTLSLTDFGYFKKPDGSIYWIGVEKNHVLTQTYN
                     QLCDGLSKSGFKIEQREYRPHITLGRGIILKDNFDREQFVRAIPTMNMAVEKISLMKS
                     EQIRGQLIYTEIYSVIL"
     gene            7117..8430
                     /locus_tag="PK733_06270"
     CDS             7117..8430
                     /locus_tag="PK733_06270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ10182.1"
                     /translation="MGKKESDFRYNTGEARVPWAAVGENYNAEDLMAVIEFLMQGSGP
                     EYEEAIKAVREQVDKLSAISTPPGKLSLASKVEEAEQAVDKYLGTKGSLFVTNATAGF
                     EIAYKYANLQPGDEVIIPAITFIATMAYPLAVGAKVVFADVDPKTINMDPADVARKIT
                     PKTKMIVPVHIGGYPVDMDPIMELARKHNILVLEDAAHAFGAMYKGKKVGTIADFTAF
                     SFHEVKNITSFGEGGILTTNIEDFRDEMKRARFLGLNFSKKIKNWLYDITAIPGKYGP
                     FVASNNCSTTEIQGIGLKLQIERYDKILAERRSAAAYMTKRLSENPAIIPQDLGNEDY
                     APTFHLYLLQIDPEKAGGDIQVLKEKLEKKSITNIPHFGPLYRFDVLRTLGYDEDAIA
                     ATCPVCEEVFYKRFTHLPIYGLSEEQLKYMADTILESVAEMQCGE"
     gene            complement(8625..10049)
                     /locus_tag="PK733_06275"
     CDS             complement(8625..10049)
                     /locus_tag="PK733_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ10183.1"
                     /translation="MNDNKTNDKRNYVFRIITFPIYMISYLIKKAAEKRRFSIRLSLS
                     LVYLKIVIRTLVLTGILIFLAYGGIRIYYIYQNYKLIVEKVITSQKIESGTLKDVLDE
                     KRHIAVYDKDENYLFDTAGSDLEIYKHKHIAYFTVIDNSYFLILDKKVLLSGEIFFLN
                     IYTNVTTEAKDIYNLLKIILLANIIGTIFAGIWSIFAGRNVFLPIREMTETARSISEK
                     NMNVRLNISGSKNELKDLARTFNEMMDRIEDGYNRQKQFVSDASHELRTPIAVLQGYI
                     DMLNRWGKNDREILQESIDAIKNETENMKDLVENLLFLARNDRGTLILQKEEFSLTNL
                     LEETIKETVLIDKRHKLSCNIKNGVNIYADRNRIKQAMRIFLDNAIKYTPEAGEITVN
                     LYADKNYAAIEIIDTGIGMSEDEMKHIFDRFYRSDKSRKRERGGHGLGLSIAKIIILS
                     HDGKINVKSKPVEGTRIKVFFPYK"
     gene            complement(10042..10725)
                     /locus_tag="PK733_06280"
     CDS             complement(10042..10725)
                     /locus_tag="PK733_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10184.1"
                     /translation="MKGVILIVEDEAKIARFLELELKHEGYEVEVAYDGRTGYEKAVG
                     VNIDLIILDLMLPELSGIEVCRRVRRVSDVPIIMLTAKDDISDKVMGLDMGADDYVTK
                     PFAIEELLARIRAALKRTANTERKNSDIIAAGKLELSRNEYKVKYADEEIMLSKKEFE
                     LLLYLMENKGIVLTREKILENVWGYDFMGDTNITDVYIRYLRSKIDQRFGVQYIHTIR
                     GVGYTFRYE"
     gene            complement(10999..11529)
                     /locus_tag="PK733_06285"
     CDS             complement(10999..11529)
                     /locus_tag="PK733_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOJ10185.1"
                     /translation="MSIPMEKIDELRKRTNCSYEEAKTLLEKHNGDLLEAIVEFERKN
                     SSRSNSNYYNGTVSFGTKVKQLIDKGFRTRFIVEKSGETLINISINLLILAALILNWI
                     LLIALIISFILGCRFRIRKEKGESIDLNKFIDGFGSKVKEASGEAKQQNNPNGAKNPD
                     KRNDDNDDGYNEITIE"
     gene            complement(11786..13933)
                     /locus_tag="PK733_06290"
     CDS             complement(11786..13933)
                     /locus_tag="PK733_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10186.1"
                     /translation="MQTPKFIYILRACIQPGHYEEKRLDTLISFCRQACIDDVMFFID
                     CEDLNQGHITSDEVKLWLQLIARAGEQLTPLGITTSVNPWTTLNHADRGRTLKEGQKF
                     QLMEDPCGTQSTVCACPLSKEWRKYITDIYSMYASIHPYMLWIEDDFRFHNHAPLQWG
                     GCFCDLHMEEYSKRAGKKLTREEFIQGILQPGEPHPYRKIWLDICRETLVNVAQEIGE
                     AVHKVSPNTRLGLMSSIPEVHCAEGRDWEGILKGLATDTPMVNRPHLPSYQEIGPQSY
                     FWYFNTISVMSKALVPASTEIYPEVENSPRTLFAKSRNFTRFQIESSAVLGSQGITLN
                     LFNVMGSGVLPKEGFQRMLAETKPFMDKIAELNIGKGKREGIKVLFSPLSSYTLYTSK
                     GEKMEELYPVENFWAGLLSAYGIANCYSSDKNIENEFIAISGQYFRNLTGQEIFHLFE
                     KNFVLLDGEAAYTLYDMGYGDIAGIGGMKWYKTHSGVQAYEEVCNGIEYCGINNARMS
                     LQRAEGGYYKIDYIKEPYLITQAKSPNGEIAGPGMCIIDNKIFILPCGGKDFFFQIHL
                     NTYKQAILQSVICGELQSYMQDSAQINTGSFNKPVYAEQEPYVGVYVYDYSGSKVILL
                     VNGSGDDLENLKVHVPEAFQANCHKGHDDYKPHEPHESSKSHEFYDVDVYEISRNDVQ
                     EKEIPFNYKPGEIIIETTLDRFEVKAFILKART"
     gene            complement(14021..14491)
                     /locus_tag="PK733_06295"
     CDS             complement(14021..14491)
                     /locus_tag="PK733_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ10187.1"
                     /translation="MPDSSTNVDNVKRKLRSLKKLEVKIRFGDSFQEGNLYAKPPKML
                     LVWDEYFDINSANRGKAKYSLEDIASMDKDEFKDIVDEFFFRVYYMYYIENGIANFTF
                     YNPEILKWMDLPYNSGIEEIKKKFRQLAKRYHPDTGGSSSEFIELMEKYRKLIE"
     gene            complement(14603..>15228)
                     /locus_tag="PK733_06300"
     CDS             complement(14603..>15228)
                     /locus_tag="PK733_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10188.1"
                     /translation="GNKKYSEPYQNTRKLPNYPNLPNSPNNVVRIPSANFSFFKYLFF
                     FFILFIIISSKRRKYYRRRFGIPFNPFSWIRIRRHGSGGYWGRYGPPPPGGGFWYNGR
                     WNSKPRSQSGPGWNTPPRPGPGPGSGSGSGPFSSGGSPFGRSGGGGSTRGGGAGRSSF
                     GRSSFGGGSSFGRSPFGGSKSFGGGKSFGGRSGGGGFTRGGGAGRRK"
     assembly_gap    15229..15238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15239..15822)
                     /locus_tag="PK733_06305"
     CDS             complement(<15239..15822)
                     /locus_tag="PK733_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOJ10189.1"
                     /translation="MSRKTLKIIIFSFLLVILFPSVGFGKVTYPEPSTKFYVNDYANV
                     IDEEAENLMVQNGNALEERTGAQIVLVTVNFTDGISIDEYAAELFNKWKLGSAEKNNG
                     LLILLSIGDEDYWTVQGKGLEATLTSGDIGQILDDYLEPDFAVADYSEGAVKVYEAFI
                     KELGGSWVGKTDSGNVGDSKNNGNTENYGNNGNS"
     gene            complement(15947..16495)
                     /locus_tag="PK733_06310"
     CDS             complement(15947..16495)
                     /locus_tag="PK733_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ10190.1"
                     /translation="MEFLKKRTNAWLITLIIIILATVISSGSSLNKLRLEAENVFYIG
                     EDMDGIGIQHDLEMIMSDCSNLTVVAGRYMDKEHELIKAVLKARDTLGAAKTPGKKYR
                     EAQNLLQSAVELYTYIGDLPLSQKDQDFRRSLYTNMKSHNIIISRSTYNQHAREFNNI
                     LSRFPANILSAVSFISPLELYE"
     gene            complement(16671..17855)
                     /locus_tag="PK733_06315"
     CDS             complement(16671..17855)
                     /locus_tag="PK733_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HOJ10191.1"
                     /translation="MDKNNISSSANNGINNSIYNGALVPELVLNPVSNDRKQEDILAT
                     ERVNIENSGLTEHEKAMVKDFSQKIDISDSSQILQYGNSAQKKIADFSENALANVRTK
                     DMDEVGKMITDLVVELKDFSDETEAKGFFGLFKKAEKSISRLKVQYDSAEKNVDKICE
                     ILEGHKITLIKDITMLDRMYEMNLAYYKELSMYILAGREKLEDVMNKELPALQEKAKM
                     SGKPEDAQAANYLADMCNRFDKKLHDLELTRTISVQMGPQIRMVQSTDSIMVEKIQTS
                     LVNTIPLWKNQMVLALGMAHSKSAIQAQRQVTDITNQLLRKNAEMLKTNAVEAARESE
                     RGIVDIETLRQTNQALIETLDEVMQIQRDGRNKRREAEKELVRIEDELKNKLLSVRDA
                     TR"
     gene            complement(17919..18542)
                     /locus_tag="PK733_06320"
     CDS             complement(17919..18542)
                     /locus_tag="PK733_06320"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HOJ10192.1"
                     /translation="MREIKVKSVIPIYSIGLVWILCALVFPMYRWYDLAIALGISVVV
                     YKITSRLIPSRTILIESEPEPISTGNSELDEILTAGTAYMSELSSLNMEIANEKINKQ
                     VNEMMRITKDIFEFIRGNPTKVRQIRQFMNYYLPTTVKLLKNYNEFRKQEAKGDNITS
                     AMQKIEGVLDTIVDAFRKVLDGLFMDKALDTSVDIEVLEKMIREEGF"
     gene            complement(18724..19515)
                     /locus_tag="PK733_06325"
     CDS             complement(18724..19515)
                     /locus_tag="PK733_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ10193.1"
                     /translation="MNIELIRNKSGFICDMDGVIYHGNHLLQGVSEFVNWLKSEKKEF
                     LFLTNSSERSPKELEQKLKRLGLDVDESHFYTSALATASFLAGQCPGGSAFVIGEAGL
                     INALYDAGFSMNDINPDYVVVGETRSYNYERIERAVHLVYGGAKLIGTNPDLTGPTEK
                     GIVPATRALISPIELATGKQAYFVGKPNPLMMRVGLKRLGCKSDETAIIGDRMDTDII
                     AGIESEIDTVLVLSGVTNMDNINNFPYSPKYILNGVGDIVGRSWQ"
     gene            complement(19785..20648)
                     /locus_tag="PK733_06330"
     CDS             complement(19785..20648)
                     /locus_tag="PK733_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10194.1"
                     /translation="MKKIGLIDYYLDNWHASNLPKWLDDETHGEYKICYAWGSLNPDG
                     SESGKECCKPSEVWCAEHNIELVSSAAEVVEKSDVLMVLAPNNPEQHEELCREALASG
                     KRTYVDKTFAPDVATAKRIFDMARAGGTPMFSSSALRFSSELKDIKREGICVISSRGS
                     GVYQIYSIHQIEMLVSLMGPDAKRVMAIGNEAAPALAIEFSGGRFATMGHFGWECPFN
                     LAITYDSSENVLVRECTSFFPNFVHELVDFFETGSIKAPEKETIAVIGIREKGFEALK
                     NPGVWISCELD"
     gene            complement(20806..21864)
                     /locus_tag="PK733_06335"
     CDS             complement(20806..21864)
                     /locus_tag="PK733_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10195.1"
                     /translation="MDKVKVAIIGCGTIANSAHIPAYVNNPDVEILYFCDIIQERAQA
                     AVAKYGVGKAVTDYKQVLADPAVEAVSVCTPNNMHPVISIDALKAGKNVLCEKPAART
                     YTEALEMQKAQHESGKVLNIGVVNRFRTAVVKVKELIDAGELGDIYHVYASFRAHRSI
                     PGLGGDFTNSAISGGGVLIDWGVHFLDLVMFCLGDPTPKTVSGQAYSILGKNIKDYAY
                     TSMWASEPKFDGIYDVDDFITGLIRTEKATISLNGAWAQNIGESEMYIDFLGDKAGIR
                     LQYGGNFKLYTFKNGTLLEVIPSFKTDKEFQAEIDAFVKCIRTGEKLPSHIDTVIITA
                     KIMQALYDSSKANKEIVF"
BASE COUNT         6505 a         4551 c         3414 g         7455 t
ORIGIN      
        1 aaagagcata ttttaaaact gaagtctgtc tgcgctcatt agtctgtaaa acatcagata
       61 ttacctccgg caggaaccca tcgaatttta cctccaaaat aatgagtcct ttatcaagag
      121 tttcgattgc tggaaaatta ttgtcaaaaa actttgttga aaaaagtcct gttctaaccg
      181 atttatcaaa agttattcgc acattgcccg caggataggt atatgcttcc cgcacataat
      241 caacaatggt tttaggtttt aaaagaccgc tttgcatttt aacgtaaaat tcggtaacaa
      301 gcgagacctc actttctctc atccatccaa tatcacctat taaaagtttt tcacattgag
      361 tcctcgaaac cacagcagaa aacttagctg taaggttatt gcacttttgc tttttctcaa
      421 gccgaatcag tgatgtatcg ccattataaa agcgtatcct gaacttttct ctgttattta
      481 ccccagagat tttttcatat agtgcactgt cataaaaatt atcaaagtac aagctgcgta
      541 taatatattc attatttaca tcgccatgta tatcagcttg catgactgcc ttgaatcggt
      601 tcctcataat ataataatcc atcatattta tataatgctt gatttcatgc cgaaatttta
      661 tagcctccat attgctcacc ccccaacttt tctaaacttt ttaatatgca aatctgcttc
      721 accttataca agagattgca gagatccgct ttcccgagcg gcctcctatg ttttgttctt
      781 tatatgaatc cgtgagtgaa gcaagtttgc tgtaatttaa tgttttgcat gtattcattc
      841 tatattattg tgagaactaa tatctccgca aaattgtggc aaaattgaag cagaagcatc
      901 taaaatatga tatttcacat atttcatgat aaaatattgt tagaggtgaa tatagtatta
      961 tggataagaa gttaatatat atagctgatg atgaagtcaa catttgcaat attataaaat
     1021 cctttcttat taaagaagga tatgaagttg aagcctttaa tgaaggaggc tctctgcttg
     1081 atactttcaa taaaagacat gcggatatgc ttataataga tattatgatg cctggaatag
     1141 acgggttctc tctctgcaca tcaatccgcc aaaagagtaa tgtcccaata atcatagtat
     1201 cggctaagga tactgagcct gataaaatag caggactttc attgggaagc gacgactacc
     1261 ttacaaagcc atttagtcca ctggaactca ttgcacgggt aaaaagcatt tttagaagaa
     1321 tcgagttgga taaggcatat gcaggtaata atgagtcaat aagaatttct gatattctta
     1381 ttgatcccaa taataaacaa gttgaatttg gtggaaaaaa tataggactc acaagaatgg
     1441 aatttaattt gctttattat cttgcagaaa acactaacag agctgttggc cgaagtgaac
     1501 ttctagataa ggtgtgggga tttgaaaatg aagttgaaac cagagctact gatgatatgg
     1561 taaaaagaat ccggaagaaa ctttctgatg ccggttcagt tttaaaaata gatacagtgt
     1621 ggggatttgg ctttaaaata gaaaataaag agtgatattg tgcaaataaa ttttcacatt
     1681 attaattaat acttatgcct agtacagtag cggcagaatg gagattatta tgaagaataa
     1741 tacaataaaa tggaggattt ttaaatacaa tcttatagta atagtactgc taattacatt
     1801 gactactata atattcaatg ttgctgtacg tctatacatc gaaaaagaca taatcagcca
     1861 gctgcacaaa atcgcatcac ggactgaagg cactgttctg gaaaaaggcc ctgacttttt
     1921 cccaaaaagc agaggcatgc aaccaccgcc tcctcctgac atgcaatata ataaccctcc
     1981 agacagagtt tcagagtatt ttaattttca agacgataat gaactgttcc ggttttattt
     2041 tatgctggac cgctcgttga gagaacctct ctccgtttta aatgccgatt atattttact
     2101 tgataatggt aaaaatataa taaatactcc tccaatggaa tactttaacg cttccgatga
     2161 cctaatgaac aaaataacta atgagttgag caaattgaaa gacttaacca ttgaaacata
     2221 catgagcttt catttttccg gaaccgaata tgtagcaatc gtcaagccgg ttaccaataa
     2281 gaatactttt ggattgggct ggatagttat atattccagt ctccaaaagg ttaatcagct
     2341 gcagtgggta ataaatctca ttctttttgc catacttatt ttatcggcaa ttgtaattat
     2401 aatcttttct tcacttgctg caaaaaaaat atctgcaccg ttctcatctc taaatcagca
     2461 cataagggct attgcagaaa gaaagttcag ctccaagatt tccatgcctg tagatgatga
     2521 actgcaggaa ttagtaaaca atataaatgt tatgtcagaa aagcttgaaa catacgataa
     2581 agcacagaaa accttcctgc aaaatgtgtc ccacgagttc agaactccgc ttatgtctat
     2641 tcagagctat gctgaaggca taaaatatga tgttgtgggt agcagttccg ctgttgacat
     2701 aataattgac gaaaccaagc gtatgactca tctggtagaa gatttgcttt acttgtcgcg
     2761 tcttgatgct attgaagaaa gttatcactt tagtaatttg gatttggatg attttatgaa
     2821 tagttgtatt gagagaataa atgggattgc cataaaaggc aatataagaa taactgtcaa
     2881 taaccttatg gaaaaggttg aattatatgc agacgaggaa aagctttcac gtgcaattac
     2941 aaatataatc agcaattgta taagatacgc aaagaatact gtagcaatta cttcaaaaat
     3001 tattgatgtc aataaaatag agctttctat ctctgatgat ggtccaggct ttgatccgaa
     3061 tgagctttcc aatatatttg aaagattcta tagaggcaaa aagggaatat tcggactggg
     3121 cctggccata agcaaaaacg taatagagaa acataagggg gaaatcactg cagcaaactc
     3181 tgaatcaggc gccatattca taattcaatt gccaatatta tcgaaacaat aatcttcgta
     3241 aacttcaatg agttttcaat aattgcagca atgcatgtaa cgaagttgtg tgccagcttg
     3301 tccaaaatag aaactgcagg tgtatactta tgatttgaat aaactatgtc tgaatgtatt
     3361 tcatctgaag tacaacttaa atatcttttc cgtacagtcc cattttaaag agcatgtgtt
     3421 tctgcgatcg tgtgttatat caaatttata ttccattata tcccattcca acttccatta
     3481 gatatccttt tagtcatgct ttaagttata cttttagtta tgctttgcca ttactactgc
     3541 cctgcctatt attatatctt cagtttcatt gataaattgt cctgcaagtc gtgctgaaat
     3601 aaatgaagca ctttcatagc ctccattaag caaaacatct tttgtaggta tatagcacat
     3661 aatgctgttt gaatacccga gaacaattgc atcttcatcg cttaatattt tcttgatctt
     3721 ttttccgatt tcagatacaa cttcattttc aagtcctata atacatgttt tgtcatccag
     3781 tcttaaaagt gatatatagt atgggatata acttttgatt tttccattct tcatctcgtc
     3841 tataaccttt tttgcagcta tttttctata ttccggttca tcaggattat tggcaatagt
     3901 ttcccacttc tctatgctcc atatttctga atataacttt acttcactgg attctgtttg
     3961 aagttctaca tttatttttt tccaaccggc attatcaata agttcctgaa tctctcctgc
     4021 aagggatttg ccggcttgat caacttcgtc taaggtacag cttctaaacc ttccattgtc
     4081 tgctgttata tatggcttta tatcagcgcc acatccctga aggaacatgg gtatcatccc
     4141 ttcatacttt tcctctaggt atttacgcac cattccagga taatctgcag atatccagta
     4201 attatcaggc ccaagggtgg ttggatggca ggcataatta tatataagtc ctcttatttc
     4261 attactctta tcaacaagct ttataagaaa taaatcttta tctatagcat tttcattaaa
     4321 atatggcttc cataaggctc cctgttcaga tggatatctt ctgctcacgc caaatttcga
     4381 tttgcccttg caaatataag catctccctc tgtcagatta tcaaaagctt tatcaaccgc
     4441 ctccataatt tttgttttaa cagtgttgta gtatattaca ttttcaggat tatcgtcatc
     4501 ttctcccttt atactgatta cagaatgtgt atgggcgtag ttgataatta tttcatcctg
     4561 caccaatccg tacttttcat ttatagcttt tttaatttca tctgctactc tcctgtttcc
     4621 atacaacaaa tccattgcta taattgctac agtcttattt gactgaatta atgcaactgt
     4681 ggcatagata tcatcattta ctcctatact ttttttccgg gctcttcctg aaaagccttg
     4741 ttgaaagact ggaaattccg gagtaatctt ttcctttaat attgaaaact tcacactaac
     4801 gcctcccata aattaatatc tgctaataac cagcaatggc tagcagcaaa cagtaataat
     4861 taactggttt atttagataa ttattaataa atatacacat ttaaacccct cattataagg
     4921 atactcctaa ttataatatt attcaattgg ttttatcatt ccttaacact acttgccaat
     4981 atacctttta caaaatattt ctatatgaaa agatatattg caactatagg caaagctaca
     5041 agtatgatag atgcaatcta gtagttttga gggggttggg atttcaacgc ttattatttc
     5101 tttttatatt ctttctcttg gtcactgaaa aaccaaaata tcctatagat ttctttaaac
     5161 tccagtattc gccatgagaa tagcaaataa ggcccgcttt ttcaaggcaa agtttcccat
     5221 attcatctat taaagcttca tcaacatcta cagccacaat ttcaccaata aacataacat
     5281 gagatccaag ctctacagca tttctgacta tgcactctat attcaccggg ctttccccta
     5341 tcaacggtac atctactctg gaagcctttt cgggagtcag gtttgcagca gcaaacttgt
     5401 ccacattacg gcctgatttt acaccacaaa aatcagtggc aaaagccagt ttctctgtag
     5461 tcagattcac tacaaactga cctgtttctt ctaataattg gtaggaataa cgttccttcc
     5521 gtactgaaat tgataccata ggaggattag aatttatcgt acctacccat gccagtgtta
     5581 taatatttgg tttcccgtct ttccccatac atgtaaccat aactgcagga acagggttca
     5641 atatagtcga cggcttccaa acctgctttg ccatatatat ttctcctttt aatcccaatt
     5701 tctttgcatc caattatgat tctatcataa aaacattaaa tatgacagat gtagatgcga
     5761 cagcgacggt gtaacgggga tgtaacgggg acagttctgc tctatctatc atcaatatct
     5821 atcatcagta tctatcatca ataatatgca agatttaaaa gacccgctat aactcgctta
     5881 acgcttaata gcaggccttt tgtttattag ttatgcatcg ttttcagatg gtttttacat
     5941 tttacttcca ggtataaatg ttacacaatc tgtttcctga gaatctgccg cgtttctggg
     6001 ctgaacttca attctttctg ctgcacagtg atctcccgaa agataataat ggcatgtatt
     6061 aactacacat ttaatgcccg gattggcgtg ctccattttt ttagtggtca taaattatcc
     6121 ctcctaatag ttttttcatt attattttta gcagttacgt ttttttaata cggaaattaa
     6181 taaaaaacca caacgccgca aagagagaac tgtatcccaa aagctaaaag ccaatttacc
     6241 aagaaactac aaaaactaca atatcacact ataaatttca gtataaatta gctggcctct
     6301 tatttgctct gatttcatca agctgatttt ctctaccgcc atattcatgg ttgggatagc
     6361 tcttacaaat tgctccctat cgaaattatc tttcagaata attcctctac ctaaagtaat
     6421 atgaggccta tactctctct gttctatttt aaatccgctt ttacttaacc catcacaaag
     6481 ttggttatag gtttgagtaa gcacatggtt cttctcaacc ccaatccagt agatactccc
     6541 atctggcttt ttgaaatagc caaagtctgt tagcgataaa gtaaatttat tcttctgtat
     6601 actattcatt acttctttca ccttgttaat tctcccggtc tcaacttctc ctaaaaagac
     6661 aagagttaaa tgaagatttt cttgctttgt cattctcccc tgcattgact gagcttgtaa
     6721 gctctgtata acattataaa gccttttttt tatatcatcg ctaaagttga ttgcaataaa
     6781 taatctcatg taataaccct ccatggacta cattattttt aataaatcta tctgctataa
     6841 tgcctataat aacatctttt caaaatatag tatattctat tatgaatatt ctatcatgca
     6901 ataattagta tgtcgaaata taattataat tgttacctta attgttacct taacaataaa
     6961 ttctaatcta tactattgaa attcaaccga atatatgcta tatttttatt aaaggaaaca
     7021 ctagcaatat ttttgttcat aatttgttta tgcaataaat aaattaatta attaacttat
     7081 taatttatca aaattgtaag gaaaaggaga attattatgg gaaaaaaaga atcagatttc
     7141 aggtacaaca ccggggaagc tcgtgtaccc tgggcagcag tcggagaaaa ctataatgcc
     7201 gaagatctta tggctgtaat cgaattcctt atgcaaggca gcggtccgga gtatgaggaa
     7261 gcaatcaaag ccgtcagaga acaggttgac aaactgtccg caatttccac tcctcctggg
     7321 aaactctctc ttgcatcaaa ggttgaggaa gctgagcaag cagtggataa gtatctcgga
     7381 actaaaggca gtctatttgt taccaacgca actgcaggtt tcgaaattgc ctacaagtat
     7441 gcaaatctcc aacctggtga tgaagttatt ataccggcta ttacattcat cgcaaccatg
     7501 gcttacccac tggcagttgg tgcaaaggta gtatttgcag atgtggatcc aaaaactata
     7561 aacatggacc ctgcagatgt agcccgcaaa attactccaa aaaccaaaat gattgtccct
     7621 gtgcatattg gcggctatcc tgttgatatg gatcctatta tggagttggc cagaaaacat
     7681 aacattctcg tgcttgaaga tgcagctcat gcttttggtg ctatgtacaa agggaaaaag
     7741 gttggtacca ttgccgactt cacagctttc agtttccacg aagttaaaaa tattacatct
     7801 ttcggtgaag gcggcatttt gacaaccaac atcgaagatt ttcgtgatga gatgaaacgt
     7861 gcaagatttc ttggcttgaa cttctcgaag aaaattaaga actggctgta tgatatcact
     7921 gccattccag gcaaatatgg tccgtttgtt gccagcaaca actgttcaac taccgaaatc
     7981 cagggtatag gtcttaagct gcagattgag cgttacgata agattcttgc tgaacgtcgt
     8041 tccgctgctg cttatatgac taagcgtctg tctgaaaatc ctgcaattat tcctcaggat
     8101 ttgggtaatg aagattatgc tccaacattc cacctatatc tgcttcagat tgatcctgag
     8161 aaagcaggag gggatattca ggtacttaag gaaaaacttg aaaagaagag cataactaat
     8221 attccacact ttggacctct atatagattc gatgttcttc gcacattggg ctatgatgag
     8281 gatgctattg cagcaacttg tcctgtatgt gaagaggtat tttacaaacg attcactcac
     8341 ctccccatct acggactttc cgaagagcag ttgaaatata tggctgatac tattttggaa
     8401 tctgttgcgg aaatgcaatg tggagaataa atatctcagt agatttgctt cattaaggga
     8461 ttaaaatagc tattattcgg gtgcatttaa tttatctgag gaaaatattg gcaaagggca
     8521 taagtgaagg ctctaagaac cgtgacgata gcccctgata aatgtttttt cagatttggt
     8581 aaatgcaccc ctattattat agcgaatttg acagcttaaa agtttcattt ataagggaaa
     8641 aagaccttta tcctggttcc ttctaccggt ttgcttttga catttatttt cccgtcatgg
     8701 ctcagaatta ttatttttgc aattgagagt ccaaggccat gtccacccct ttcccttttt
     8761 ctggacttat cggagcgata aaacctgtca aaaatatgtt tcatctcgtc ttccgacatt
     8821 ccgattccgg tatcaattat ttctattgct gcataattct tatcggcata aagatttaca
     8881 gttatttcac ctgcttcagg tgtatattta atcgcattat ccagaaatat tctcatagct
     8941 tgtttaattc tgtttctatc agcataaatg ttaactccat ttttaatatt acagcttagc
     9001 ttgtgtcttt tgtcaattaa cacagtttcc tttatagttt cctccagcaa attcgttaag
     9061 ctgaactctt ctttttggag aattaatgtc cctctgtcat tccttgcaag aaacaataag
     9121 ttttcaacaa gatccttcat attctctgtt tcatttttta ttgcatcaat agactcctgc
     9181 aatatttctc tatcattttt accccagcgg ttaagcatat caatgtatcc ctgtaatacg
     9241 gcaattggcg tccgcagctc gtgggaagca tctgaaacaa actgtttctg cctgttgtat
     9301 ccatcttcta tcctgtccat catttcatta aaagtccttg caagatcctt aagctcattc
     9361 tttgaaccgc tgatgtttaa tcgtacattc atatttttct ctgaaatact tcttgcagtt
     9421 tccgtcatct ctcggatggg taaaaataca ttccttccag caaaaatact ccatatacca
     9481 gcaaagatag ttccaattat attggctaat aaaataattt ttaagagatt gtaaatatcc
     9541 ttggcttcag tagtaacatt cgtatatatg ttcaggaaaa aaatttctcc cgaaagcaat
     9601 acttttttat ccaatattaa aaaataacta ttgtcaatca cggtaaagta tgcaatatgt
     9661 ttatgcttat atatttccaa atccgaacct gcagtatcaa ataagtagtt ttcgtcttta
     9721 tcatacacag ctatatggcg tttttcatct aaaacatcct taagtgttcc agattcaatt
     9781 ttctgacttg taataacctt ttcaacaatt aacttgtaat tctgatatat ataatatatt
     9841 ctgattcctc cataggcaag aaaaattaat ataccggtta aaactaaagt tctaataaca
     9901 attttcaagt acactaaaga taagctcagt ctaatagaga accttctttt ctcggcagct
     9961 tttttaatga ggtaagatat catatatatt ggaaatgtta ttattctaaa tacatagttt
    10021 cttttatcgt ttgttttatt gtcattcata cctgaatgta tatcctactc ctcttatagt
    10081 gtgtatgtat tggacaccaa acctttgatc aattttactc cgcaaatacc ttatatatac
    10141 atcagtgata tttgtgtcgc ccataaaatc atatccccac acgttttcca aaattttttc
    10201 tcttgttaat actatacctt tattctccat caaatagaga agcagttcga attctttttt
    10261 ggatagcata atctcttcat cagcatattt aaccttatac tcatttcttg acagttccag
    10321 cttacctgct gctattatat cactattctt tctttctgtg tttgccgttc tttttaatgc
    10381 tgccctaatc cttgccaata gttcctctat tgcaaaaggt ttggttacat aatcgtctgc
    10441 tcccatgtca agccccataa ctttatcaga aatatcatcc tttgcagtta acataatgat
    10501 aggtacatca gatactcttc taacgcgtct gcatacttca attccgctaa gctcaggaag
    10561 catcaggtca agaataatca agtcaatatt tacgccaact gctttttcat accctgttct
    10621 tccgtcataa gccacttcca cttcataacc ctcatgtttc aattcaagtt ccaaaaacct
    10681 tgcaatttta gcttcatctt caacaattaa tataacgcct ttcataaaat tcatatcctc
    10741 tttattagaa attaaactac agtatttaat actactttgt tatgttggca atagaaagcg
    10801 catttccgga tggtaatttt tgctggtaag taaggaatat atcatcccaa tcctatcaaa
    10861 aaatatcctg gaagacatcg ttttccaaat tggtatagta gtattaagct tcactcttta
    10921 attatatcag aaaaatttct cctggtatat taaaaaccaa cccgcttatt atagcaggtt
    10981 ggtctttaat acattaattt actcaattgt aatctcatta tacccgtcat cattatcatc
    11041 gttcctttta tcagggtttt ttgcaccatt cggattattt tgctgttttg cttctcccga
    11101 agcttccttt accttgcttc caaatccgtc aataaatttg tttaaatcaa tactttcccc
    11161 tttttcctta cgtattctga atctacatcc aagaataaaa gaaattatca atgctataag
    11221 taggatccaa ttcagtatta atgctgccaa tataagcaga tttattgata tgtttattag
    11281 ggtttcaccg gatttttcaa caataaatct tgtcctaaag cctttgtcta tcagttgttt
    11341 gacttttgtg ccaaagctta cagtaccatt ataatagttg ctgtttgatc ttgaactatt
    11401 ttttctctca aattcaacaa tagcttcaag caaatctcca ttgtgctttt caagaagtgt
    11461 tttagcttct tcatagctgc aatttgttct ttttctaagt tcatcaattt tctccattgg
    11521 aattgacata tgcttcatcc tctctgttta taattttttt acattgtaat tacatatttt
    11581 atgattacac actttgcaat aacacatttt gtagttacat gtttattata gattgcgata
    11641 attaattaaa tcttaatata aaattagaat ttgattagaa ttgctttcac ttgtgggttt
    11701 tttaaaacac tgaaagaata ctggtataca gctaatggcg gctgctatta gctgtatact
    11761 tgacattttg aaaataaatg tgccattatg tccgcgcttt taaaataaaa gctttaacct
    11821 caaatctatc cagtgttgtt tcaattatta tctcaccagg cttgtaatta aaaggaattt
    11881 ctttttcctg gacatcattt ctgcttattt catacacatc tacatcataa aattcatgag
    11941 atttgctaga ttcatgaggt tcgtgaggct tataatcatc atgaccttta tgacagttcg
    12001 cctgaaaagc ctccgggaca tgtaccttta aattttccag gtcatcaccg gatccattaa
    12061 caagcaatat tactttactg ccactgtagt cgtaaacata tactcctaca taaggctcct
    12121 gctctgcata aaccggttta ttgaagctgc ctgtattaat ctgtgcacta tcctgcatat
    12181 aactctgcag ctcaccgcat attactgact gtaaaattgc ctgcttatag gtatttaaat
    12241 gaatctggaa aaagaaatcc tttccaccac agggtaaaat gaatatttta ttatcgatta
    12301 tgcacatgcc gggacctgca atttctccgt taggactctt agcctgggta atcaggtaag
    12361 gttcctttat ataatcaatc ttataataac cgccttcagc tctctgaagt gacattcttg
    12421 cattgttaat tccacagtat tctattccat tacaaacttc ttcataggct tgtactccac
    12481 tatgggtttt ataccacttc atgccgccaa ttcctgctat atctccatac cccatatcat
    12541 aaagagtata tgccgcttct ccatcaagga ggacaaaatt cttttcaaat aaatgaaata
    12601 tttcttgtcc cgtaagattt ctaaaatact gtcctgaaat agctataaac tcattttcaa
    12661 tgtttttatc actactgtag caattggcaa tgccatatgc tgaaagcagc cccgcccaga
    12721 aattttctac gggatatagt tcttccatct tttctccctt agatgtatat aaagtgtagg
    12781 aagataatgg gctaaacaaa acttttattc cttcccgttt tcccttccca atgttaagct
    12841 ctgcaatctt atccataaaa ggcttcgtct ctgcaagcat tctttggaat ccttcctttg
    12901 ggagaacccc gctgcccatt acattaaaaa ggttgagagt aattccttgt gaacctaata
    12961 ctgcagaaga ttctatttgg aatcttgtaa agtttcttga ttttgcaaat aaagtacggg
    13021 gagaattttc tacttctgga tatatctccg ttgatgcagg taccaatgct ttactcataa
    13081 ctgagatagt attaaaatac cagaaataag attgcgggcc aatctcctgg taacttggca
    13141 gatgcggccg gttaaccatt ggtgtatcag tagcaagccc tttgagaatt ccctcccaat
    13201 cccttccttc cgcgcaatga acttccggta ttgaactcat aagtcccaac cgggtattcg
    13261 gggacacttt atgtactgct tcaccaatct cctgtgcaac atttaccagt gtctctctac
    13321 agatatccag ccatatcttt ctataaggat ggggctctcc aggctgaaga atgccttgaa
    13381 tgaattcctc tcttgtaagc tttttccctg ccctttttga atactcttcc atatgtagat
    13441 cacagaaaca tcctccccat tggagcggtg catgattatg aaatctaaaa tcatcttcta
    13501 tccagagcat atacggatga atagaagcat acattgaata aatgtctgta atatactttc
    13561 tccattcttt acttaaggga caggcacata ctgtagactg tgtcccacaa gggtcttcca
    13621 ttaactggaa tttttgtcct tctttgagcg ttctacctct atctgcgtga ttcaaagtag
    13681 tccatgggtt gactgatgtg gtaatcccta aaggggtcag ctgctcacct gctcttgcaa
    13741 tcaattgaag ccagagtttt acctcatcgc tggtaatatg gccctggttt agatcctcac
    13801 aatcaataaa aaacataaca tcgtcgatgc aagcttgcct gcaaaatgaa atcagcgtat
    13861 ccagtcgctt ttcttcataa tgtccaggct gtatacatgc tcttaagata taaatgaact
    13921 ttggggtttg catctcaata attacctccc gcaaattatt cagtttttga atatgattat
    13981 atcataaaaa tgaaagttca gtataaatgc agtttttatt ttattcaata agttttctat
    14041 acttttccat taactcaata aattcactgc tgcttcctcc tgtatcaggg tgataccttt
    14101 tagccagttg gcgaaatttc ttttttattt cctcaattcc tgaattatac gggagatcca
    14161 tccatttaag tatttcagga ttgtagaaag tgaaatttgc aattccattt tctatgtagt
    14221 acatgtaata tactctaaag aaaaactcat caacaatatc tttaaattcg tctttatcca
    14281 ttgaagctat atcttctaaa gagtatttag cttttcctct atttgcgcta tttatatcaa
    14341 aatattcatc ccatacaaga agcatttttg gaggttttgc atataaattg ccttcttgaa
    14401 atgaatctcc gaaacgtatt ttgacttcaa gctttttaag gcttcttaat tttcgtttaa
    14461 cattatctac attagtagaa ctatcaggca aaataattcc cccttatgct gtatttactg
    14521 ctgtattgaa attattacta aaacaaacca ccctgcttac accatttcta gtattaacaa
    14581 ggtggtttaa ctttttactt ttttatttac gtcttccggc gcctccccct ctggtaaaac
    14641 cgcctccgcc gcttctgcct ccaaaggatt ttccgccacc aaatgattta cttccgccaa
    14701 agggactacg tccaaatgaa ctgcctccgc caaaggaact gcgaccgaaa gaggatcttc
    14761 ctgccccgcc ccctctggta gatccgcctc ctccactgcg tccaaaggga ctacctcctg
    14821 aagaaaatgg tcctgaacca gatcccgaac ctggtcctgg tcccggcctg ggtggtgtat
    14881 tccacccagg cccactttgg gacctcggtt tgctgttcca acgtccgtta taccaaaatc
    14941 ctccaccagg tggtggtgga ccgtaacgtc cccagtaacc accggatcca tggcgtctta
    15001 ttcttatcca gctaaatgga ttaaagggaa ttccaaacct tcttctgtag tatttccttc
    15061 gttttgaaga tattataata aacaatatga aaaagaagaa taaatacttg aaaaaactaa
    15121 agttggcgct tggaattctt actacattat taggtgaatt aggtaaattg gggtaattag
    15181 gtaacttgcg tgtattctga taaggttcgg aatatttttt atttccgtnn nnnnnnnnat
    15241 actatttcca ttattcccgt aattttcagt gtttccgttg tttttactgt cgcccacatt
    15301 cccagaatct gttttaccta cccagctccc ccctaattct tttataaagg cttcataaac
    15361 cttaactgct ccctcactgt aatctgctac agcaaaatca ggctccagat aatcatcaag
    15421 tatttgacca atatccccac ttgttaaagt ggcttcaagt cctttacctt gtactgtcca
    15481 ataatcttca tcacctattg atagcaaaat aagcaggccg ttattttttt cagcagagcc
    15541 taatttccat ttgttaaaca actctgcagc atattcatct atagaaatac cgtctgtgaa
    15601 gttaacagta accagtacaa tctgtgctcc tgttctttcc tctaaggcat taccattttg
    15661 taccatcaga ttttcagctt cttcatctat tacatttgca taatcattaa cataaaattt
    15721 cgttgaaggc tctgggtatg tcacttttcc aaagccaacc gaaggaaata gaataacaag
    15781 caaaaatgaa aatataatta tttttaaagt cttccggctc atataccctc ctaattcttc
    15841 ttcctaattc ttcttcctaa ttttctcttc ctaattttct ctccaaattc ctgttcccaa
    15901 tttcacctct agctcttctc ataattctca atttctattc tctcctctac tcatataatt
    15961 ccaacggaga gataaaagaa acagcactta aaatattcgc cggaaagcgg ctcagtatat
    16021 tgttgaactc tcttgcatgc tgattatacg tacttcgtga tataattata ttatgagatt
    16081 tcatattggt gtataaactc ctgcggaagt cctggtcttt ttgacttaaa ggtaaatccc
    16141 ctatataggt atacagctct actgctgact gtagtagatt ctgtgcctca cggtactttt
    16201 tgccgggagt tttagccgca cccagagtat ccctggcttt aagaacagcc ttaatcagtt
    16261 catgttcttt atccatgtat ctacccgcaa caacagtaag attggagcag tcggacatta
    16321 tcatttcaag atcatgctga ataccgattc catccatgtc ctctccaatg taaaaaacat
    16381 tctctgcttc cagtctgagc ttgttgagtg atgaaccgga acttattacg gttgctaata
    16441 ttatgataat caaagtaatt aaccaggcat tagttctttt ttttaaaaat tccattacca
    16501 atcttccctt acaacaatat tatccttaca acaattttat ccttacaaaa atattattcc
    16561 tgcaaacggg tgcatatttt atttttgaat gcaccctctg caaactatta tcttgaccat
    16621 ccattatctt gattgctatt accatgatta caattgtcct gatcagctaa ttatcttgta
    16681 gcatctctta ctgataaaag cttgtttttc agttcatctt caatgcgtac aagctctttt
    16741 tctgcttcac ggcgtttatt cctaccatca cgttgaattt gcataacctc atcaagtgtt
    16801 tcaattaaag cctgattagt ttgcctcaag gtttctatgt ccacaatccc acgctcggat
    16861 tctcttgcag cctcaactgc attggttttc agcatctccg cattcttgcg cagcagctga
    16921 tttgtaatat ctgtaacctg ccgctgagcc tgtatagcac ttttagaatg agccatccct
    16981 aacgcaagta ccatttgatt cttccataat ggaatagtat taacaagtga tgtttgaatt
    17041 ttttctacca taatactgtc tgtactttga accatacgta tttgaggccc catttgtaca
    17101 ctgattgttc gtgtcagttc taagtcatga agttttttat caaatctgtt gcacatatct
    17161 gccagataat tggccgcctg agcatcctcc ggcttaccgc tcattttggc tttttcctgc
    17221 aaagcaggca gttctttgtt cattacatct tcaagctttt cacgtcctgc aaggatatac
    17281 atactaagtt ctttataata agcaaggttc atttcgtaca ttctgtcaag catagtaata
    17341 tcttttatta gagttatctt atgaccttct aatatttcac atattttatc tacatttttc
    17401 tctgctgaat cgtattgaac ctttaaacgg cttattgact tttccgcttt tttaaataac
    17461 ccaaaaaaac ctttagcctc cgtctcgtca gaaaaatctt taagttcaac tactagatca
    17521 gttatcattt tcccaacttc atccatgtcc ttagtgcgaa catttgcaag agcattttca
    17581 gaaaaatcag caattttctt ctgagcacta tttccatatt gcaatatttg cgatgaatca
    17641 gaaatatcaa ttttttgaga aaagtccttt accatagctt tttcatgttc tgtaagaccg
    17701 ctattttcta tgtttacacg ttccgtagct aaaatatcct cttgtttcct gtcatttgac
    17761 acaggattca agaccagctc cggtactaat gccccattgt aaatactatt gttaatacca
    17821 ttgttagcac tactgctaat attattttta tccatattac attccacctt cgttaataat
    17881 tttcaatcta tgctttactt atagtgctga cgtaaacttt aaaagccttc ttccctaatc
    17941 attttttcca gtacttctat atctacagaa gtgtctaaag ccttatccat gaacaggcca
    18001 tccagcactt ttctaaacgc atcaactatt gtatctaata ctccctcaat tttctgcatt
    18061 gctgaggtaa tattatcccc tttagcttcc tgttttctga attcattata atttttaagc
    18121 agttttacag tcgtgggaag ataatagttc ataaactgtc ttatctgtcg tacctttgtg
    18181 gggtttcccc tgataaattc aaatatatct ttagtgatac gcatcatttc atttacttgc
    18241 ttgtttattt tttcattggc aatttccata tttaagctac taagttccga catgtaagca
    18301 gttcctgccg ttagtatttc atccagttcg ctatttccag tactaatagg ctcaggttca
    18361 ctctcaatca atattgttct ggaaggaatc agcctggaag taattttata tacaacaaca
    18421 gaaattccta aagcgattgc caaatcatac caacggtaca ttgggaatac taacgcgcat
    18481 agtatccata ccaagccaat tgaatagata gggatgacag attttacttt aatttcacgc
    18541 acttaccaca cctcttatcc catttccagt ttataattcc ataaaaatac catattctat
    18601 aaaattagtt taacaagatt gcaagcttaa gtcaatgaaa agttctatac agttgcaccc
    18661 ttttgcacca tatattcttt cctatgctgc tatattgcta tattgattca ttgctatatt
    18721 gctttattgc caactacgtc caactatatc cccaacgcca ttgagaatat atttagggct
    18781 gtatgggaag ttgttaatat tatccatatt agttactcca cttaatacaa gcactgtatc
    18841 aatctcagat tcaattcctg ctattatatc tgtatccatg cgatccccaa taattgctgt
    18901 ttcatcactt ttacacccta aacgttttag gcccaccctc atcattaaag gatttggttt
    18961 acctacaaaa taagcctgtt tgcctgtggc cagttcaata ggagagatta acgcccttgt
    19021 tgcaggtact attccctttt ccgtaggccc tgtaaggtca gggtttgttc cgattaattt
    19081 cgctcctcca taaacaagat gaactgcacg ttcaattctt tcataattat atgatcttgt
    19141 ttcaccaact accacatagt cgggatttat atcgttcatg gagaatcctg catcatataa
    19201 cgcatttata aggcctgctt ctccaatgac aaaagcactt cctccaggac attgtccggc
    19261 tagaaaactt gcagtagcta acgcgctggt atagaagtga ctttcatcta catcaagtcc
    19321 aagtctttta agcttttgct ccagttcttt aggtgaacgt tcactgctgt ttgtcaaaaa
    19381 cagaaattcc tttttctctg acttaagcca gttcacaaac tctgacactc cttgaagcaa
    19441 atgatttcca tgatagataa caccatccat atcacatata aaacctgatt tattccttat
    19501 taattcaata ttcatatatt agttcctcat ctttaaaaat aatctttgtg ccaatatttt
    19561 atactaatat tttagttatt gggttacaat cttattataa ttttttcttc caacattagc
    19621 aagcaggata agtacgacag cgagaaaagc acagcaaaaa gcaggtctga tgtatatcag
    19681 acctgcttta aataaatgta tatgcttacg ctaaaatcaa tgtggcttgt tatcgccttg
    19741 atatcgcact gattttatta ttgctcagct aatgctaata tatattaatc aagctcacag
    19801 cttatccaaa caccagggtt tttaagcgct tcgaacccct tctcccgtat tcctataact
    19861 gcgatagtct ccttttcagg ggcctttatg ctacctgttt caaaaaagtc gacaagttca
    19921 tgtacaaaat tggggaaaaa tgaagtacat tcacgtacaa gcacattttc cgagctgtca
    19981 taggtaatgg ccagattaaa tgggcactcc cagccaaaat gacccattgt cgcaaatcgt
    20041 cctccggaaa attcaatagc cagtgcagga gcagcttcgt ttcctattgc cattacacgt
    20101 tttgcatcag gtcccattaa ggagacaagc atttcaatct gatggataga ataaatttga
    20161 tatactccag agccgcggga actgattacg cagattccct cacgttttat atcctttagc
    20221 tccgatgaaa aacgcagtgc agaagatgaa aacatgggtg taccacctgc tcttgccatg
    20281 tcaaaaatac gtttagctgt tgcaacgtca ggagcaaagg ttttgtcaac ataagtacgc
    20341 ttacctgaag ccaatgcctc ccggcaaagt tcctcatgct gctccggatt gtttggagca
    20401 agaaccatca gtacatcgct tttttcaacc acctctgccg cagaggaaac aagctcaata
    20461 ttatgctcag cacaccaaac ctcactgggc ttgcagcatt cctttccgct ctcggatcca
    20521 tctgggttaa gacttcccca ggcatagcaa attttatact ctccatgtgt ttcatcatcc
    20581 agccatttag gcagattaga agcatgccag ttatcaagat agtaatcgat caatccaatt
    20641 tttttcatcc aaactcctcc gctcatgaaa taatataggt tcaagtgttt aatctaggtt
    20701 caagtgttta atctaggctc aagtatacta aattaatgtg ataaataagg aaattaactt
    20761 tccacctaaa atagttgtga atttgcacct aaaatagttc tgaaattaaa acacaatttc
    20821 cttatttgcc ttggatgagt cgtatagtgc ctgcattatc tttgcagtga ttattacagt
    20881 gtctatatga gaaggaagtt tttcaccagt tcttatgcat tttacaaagg catctatctc
    20941 agcctggaat tccttatcag ttttaaaaga tggtataacc tccaaaagtg ttccattttt
    21001 aaaagtatac agcttgaaat ttccaccata ttgcagacgg ataccggcct tgtcacccaa
    21061 aaagtcgatg tacatttcac tttcaccaat attctgagcc catgctccgt ttagagaaat
    21121 agttgctttt tctgttctaa taagccctgt tatgaaatca tcaacatcat agatgccgtc
    21181 aaactttggt tcgctggccc acatacttgt gtatgcatag tctttaatgt ttttgccaag
    21241 tatactgtat gcctgaccgg aaacggtttt gggggtggga tctccaaggc agaacatcac
    21301 aagatccagg aagtgaacac cccagtcaat tagtactccc cctcctgaaa tagctgaatt
    21361 ggtgaaatcg cctcctagac ccggtatgct gcgatgtgct ctaaagcttg cataaacatg
    21421 gtagatgtcc cctagctcac ctgcgtctat taattccttt acttttacta ctgctgtcct
    21481 aaaacgattt acaacgccta tgtttagcac cttacctgat tcatgctgtg ctttctgcat
    21541 ttcaagagcc tcagtatatg ttcttgctgc aggtttttca catagtacat tttttcctgc
    21601 tttaagagca tctattgaaa ttactggatg catattgttg ggtgtacaaa cagaaactgc
    21661 ctctacagca ggatctgcaa gcacctgttt ataatccgtc actgccttgc ctacaccata
    21721 ttttgctact gcagcttgtg cacgctcttg aattatgtca caaaaatata aaatttccac
    21781 atcgggatta ttcacatatg ctggtatatg agcactattt gctattgttc cgcaaccaat
    21841 aattgcaacc tttaccttat ccatttacaa aacctccata ttaaaactcg tggtcacggg
    21901 gctaaaagcc ccgaaactac ggtatttttt gttag
//
LOCUS       DAOSDD010000049        21817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1044_length_21817_cov_4.937873, whole genome shotgun sequence.
ACCESSION   DAOSDD010000049 DAOSDD010000000
VERSION     DAOSDD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21817
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_21817_cov_4.937873"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK733_06340"
     CDS             <1..386
                     /locus_tag="PK733_06340"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOJ10196.1"
                     /translation="ITSAIGGAIAAANGADFLCYVTPAEHLRLPTLEDMKEGIIASKI
                     AAHAADLAKGIFNAEKWDYEMGTARQELDWEKMFSLAIDPEKAKRYRAESLPQHKDSC
                     TMCGKMCAVRNMNKVINGKNVSILD"
     gene            414..1211
                     /gene="thiD"
                     /locus_tag="PK733_06345"
     CDS             414..1211
                     /gene="thiD"
                     /locus_tag="PK733_06345"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOJ10197.1"
                     /translation="MKKVLTIAGSDSGGGAGIQADIKTFAAHGVFGMSVITAVTAQNT
                     QKVIAVQDINPEIIGKQIDAIFEDIGADAVKIGMVSQIESIKIIAEKLREYEPRNIVL
                     DPVMVSKSGYALLNPEAIDVLINELVPLATVITPNIPEAENIINAKIGTLKDVEQAAK
                     AIHMLGPENVLVKGGHMSGDSTDILYDGENIICLEAERIDTKNTHGTGCTLSSAIASN
                     LALGFSIKESVEKAKSYITTAIRHALPIGRGAGPTNHFYELYRKAGV"
     gene            1232..2128
                     /gene="thiM"
                     /locus_tag="PK733_06350"
     CDS             1232..2128
                     /gene="thiM"
                     /locus_tag="PK733_06350"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOJ10198.1"
                     /translation="MTVGNSKNKSICERIMELLEKVKAIKPLVHHITNYVTVNDCANI
                     VLAIGGSPIMADDIEEVEDIVSLSSALVLNMGTLNIRTIESMLAAGKKANQLGIPVVF
                     DPVGAGATPLRNKTAERIISELKLAVIRGNMSEIKSISGLGSNTKGVDASEKDILLSG
                     DLQYGKNIADKLSSRLGCTVAITGPVDIVTKDGKTFFIENGHKMLSMVTGTGCMCTSL
                     IGVYCGVASNYLEAAAAGILTMGMAGEAAYENIEEGNSASSGYGGYDRHGGSGSFRVK
                     LIDFVSQIRENDILERGRIYEK"
     gene            2118..2768
                     /gene="thiE"
                     /locus_tag="PK733_06355"
     CDS             2118..2768
                     /gene="thiE"
                     /locus_tag="PK733_06355"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOJ10199.1"
                     /translation="MKSKENFVDYSLYLVTERQMLRNISMEEAVEQAILGGVTLVQLR
                     EKDVTTLEFYNIAVKVKKITDKYSIPLIINDRLDIALAVDAAGLHVGQSDMPAKVARK
                     ILGDNKILGVSAATINEALQAVEDGADYIGTGAVFATDTKKDAKYVNIRQLKEITGKI
                     SIPVVAIGGINEDNIDILKGTGIKGVAVVSAIMGKENIRKASENLKKTWETCRKGR"
     gene            2892..3539
                     /locus_tag="PK733_06360"
     CDS             2892..3539
                     /locus_tag="PK733_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ10200.1"
                     /translation="MTKGIIKGAIFDLDGTLIDSMQVWGKIDLDFLGKRGIPVPDDLE
                     IKIKNLTFSETALYFKERFNIPDSAQDIMDEWNDMAYNEYAHNIKLKTGSREFLSLLK
                     LKGIKVGLATTNCKKLLEATLNKNGIFEYFDAISTADEVGKGKNCPDIYLLTAKKLGI
                     NPDECIVFEDILPAVMGAKAAGMKVIGVYDECSKHEIERIKDRADGFIISFEELM"
     gene            3622..4443
                     /locus_tag="PK733_06365"
     CDS             3622..4443
                     /locus_tag="PK733_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ10201.1"
                     /translation="MKTKQLRMLVIGAHPDDCEFKAAGTAIKFRQLGHVVKFVSATNG
                     DTGHYEIGGGQLARIRAEEVEKSCSIAGLKYQIMDIHNNGLEADIQTRERFITLIREF
                     VPDIIITHRPNDYHPDHRRTSLLVQDSSYAIIIPNVCPLTPVMPKAPVILYMQDNFKR
                     PYEFVPDIVVDIDDVFDIKTQMINCHKSQLYEWLPWTENQLDQIPQGEEERIKWLKEY
                     MLVRDGKVANRFRNQIIDKYGIEHGSNVKTAEAFEICEYGAPAEEDILNAFDSGK"
     gene            4519..6018
                     /locus_tag="PK733_06370"
     CDS             4519..6018
                     /locus_tag="PK733_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ10202.1"
                     /translation="MDTEDAFKKINEYINKAKELIKKTDFDIRTRYEDIDKNLVKALK
                     ISAKLKYELGAAQSYTYLGHLKCKLSSYDDSLEYYLNALEIYEELNDKEASASIYNDI
                     GNVYLLQENLNQALDNFIKAGEISLSVDSRNILCKALTNCGTTSFKLKNPEEALNYYM
                     KALPICTELGEKEEKCRILSNIGAIYVEFKEFNKAMKYFLDSLKIETELDSTYGIAYA
                     NFRIAETYYLMDIYEMAIAFNNKALKAADEIANKPLISYCCVLFSNIYEKSGDYKNAL
                     RYFKDYSRIREDVLEEDGKRKIADIKAKFDLYRKEKENEIYKLKNIKLKAVNKKLKKA
                     YENAEYFANIDFLTKIPNRRSIHKYINDDLINMRTPFTIVLCDIDCFKIVNDTYGHDT
                     GDYILVETSTRLNSLLADNSRICRWGGEEFLIVLPGTTLDQGISIAESLRQSISSTPY
                     LFNDKSINITMTFGVCEHNEGDSFYGLIKRADNALYNGKNRGKNCCVSG"
     gene            6157..6654
                     /locus_tag="PK733_06375"
     CDS             6157..6654
                     /locus_tag="PK733_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10203.1"
                     /translation="MIDIFEVKSIYEIEKVEELANKIWNEHYVPIIGKEQVDYMLAKF
                     QSSQAIKDQIEVGCRYFITTFNGEYAGYFAILPDKSNCKMFLSKLYVDRSFREKGIAA
                     RVVSYIEDICKESGLEMIWLTVNKYNMNSIAAYEKMGFVKEAPIVQDIGNGYFMDDYR
                     MEKKL"
     gene            6956..8128
                     /locus_tag="PK733_06380"
     CDS             6956..8128
                     /locus_tag="PK733_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ10204.1"
                     /translation="MINNINLDISRINTIIKRIIEGISSSREEVLKIVDSIRNEQENV
                     KLELCKIKNDLINVVDEVDALEKQDKYARQWLAEVSKNFNIYNENDIKNAYEKALDLR
                     VKLITKENEEKVLKDKRNNLEMTLKRLVENIDSSEKIINQISIALAYLEGDILSAFDD
                     ADKNSEMFIGIKILEAQENERKRIARDIHDGPAQHMANVAMQADICKMAIHNDLQEGL
                     KELEALKASVKEALKEVRSIIFNLRPMSLDDLGLNQTIQETVKTISQESNINIQLKLK
                     PIQGEIEPIIQVAVYRIIQEVFNNIKKHSGAKHVTVKLDFGTKYLILAISDDGIGFNV
                     EETLKRVKTRGTCYGLIGILDRVNQLQGEIRINSSEGNGTVYTVKLPVNREVIKDE"
     gene            8121..8801
                     /locus_tag="PK733_06385"
     CDS             8121..8801
                     /locus_tag="PK733_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10205.1"
                     /translation="MSKNIIKILIADDHDLIRQGLRRIISFEEDIKIIGEAENGQKVL
                     EILKFNRPDVVLLDLNMPIMNGIEALRKIKILGDDVRVIMLTVENDKKTIYEVIKIGA
                     DGYMLKDSVGTEIVDAIRTVYQGEKYIDKSLISIFFMEIRGNKSTTGATNTLDNLSKR
                     EIEVLMHISKGLSNKEIGAQLYISEKTVKNYSTSLFRKLNVNDRVQAAILAVENDIEH
                     FYKSKFQI"
     gene            9003..9176
                     /locus_tag="PK733_06390"
     CDS             9003..9176
                     /locus_tag="PK733_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOJ10206.1"
                     /translation="MLKNANNYVSSLVKQEKGQGMVEYALLVGLIAVVVIAVLVLLGP
                     AIVAKFQEIIDAL"
     gene            9332..9736
                     /locus_tag="PK733_06395"
     CDS             9332..9736
                     /locus_tag="PK733_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOJ10207.1"
                     /translation="MKMLKRELKNKKGQSLVEFAIILPLLLLVIMAIIEFGVMLNSYL
                     TIHNAAREGARAGIAGSSSTEIQNLIISVSPGLDVKDLIINISPVDKSRKPGDALTVQ
                     IRYNYHLTVPVISRVFVNNVVILSAQVSMRIE"
     gene            9752..10648
                     /locus_tag="PK733_06400"
     CDS             9752..10648
                     /locus_tag="PK733_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOJ10208.1"
                     /translation="MKKLNNNKGNTAIILCLLFTALLGFAAFVTDIGIVYAEKIKLYN
                     ALDAAALAAALELPDNIEKARKVAVEYLEKNSVDPGSTVITIGSDKKSIQIEGVKNVK
                     HLFAPILKIYSSNINAVVIAAIGPAKSVKGGIRPFAVETFDYSYGSLVTLKAGAGDGY
                     KGNYYAVALGGSGAKIFKENALYGYNSTISIGDLIYTETGNMADAANAIKNYINAERS
                     SFDDFPKSSVRLWTVPLVDTLKVNGTGQILVAGFGEFYVEDVTTNSGKIEITGRFIRY
                     VTNSDVDMTLNDTGVYGAKLVK"
     gene            10659..11618
                     /gene="cpaB"
                     /locus_tag="PK733_06405"
     CDS             10659..11618
                     /gene="cpaB"
                     /locus_tag="PK733_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOJ10209.1"
                     /translation="MKGIGQKLILISFGLAVIAAGVVFAYLQSLKESEVQIKETTILV
                     AADTIPPGTLITRQMIKQIQIPDNSILIDYIDDSSEIIGKYTKETILKNEGFHIDKLI
                     GKDEGELRLRIDSTHRAVSINVTGDSGVSDLIKPGDSVDIVVFFAEIKDGQKVIRPEL
                     SKIILQNIKLLAIDKQLNRDCTLAESKENEEIPEKFLVTLSVTTTELEKLVLAENIGI
                     LKLALRPLNNDDMDETKGATWEELTAELKNRGEFSQEDNLKSNGENSNKFINYKVKPG
                     DTLRKISREFYGDWKKYTIIKKANNIQDENLIITGQVIKIPEI"
     gene            11698..12849
                     /locus_tag="PK733_06410"
     CDS             11698..12849
                     /locus_tag="PK733_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ10210.1"
                     /translation="MDKIRILIADDIEETRNVINKILKIDNDLFDVVGEAVNGEEVLQ
                     LIPEVNPDIVLMDINMPVINGLEATEKITNEYPAVIVIIMSVQGESEYLRKAMFHGAK
                     EYIIKPFHYDELSETIKSTYEKNKERKLKFPSNREQMRNAKVIAFFSSKGGVGKSVLA
                     LNTSIILSREVNKRILLVDMDLLFGDISILINKFNQKTILDVLDDSQLNSYENIKPYL
                     YKHNENLDILFAPAKPEAAEYISKDSIDKMIKAFQTNYDVIIVDTGINFSDNTLYILD
                     CAQTILYIATAEIVTLKNTKLGLQIMKSLGYGKDKVKLVINRFTAIYGISKAEVEEAF
                     EGDVFAFIPEEGKIVCSSVNKGQPFCDNAKYRKSKIVNAFELMCRSLLN"
     gene            12903..14252
                     /locus_tag="PK733_06415"
     CDS             12903..14252
                     /locus_tag="PK733_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOJ10211.1"
                     /translation="MSLAKRLEDINIDKKNSRFKEKEADPYFDLKMKIQNRVIKELDI
                     DFNDISEQNEDLKKKIDLILTKNIEQESLNMTNNQKKKVKEELLDEIIGFGPITGLLS
                     DNRITEIMVNGPDQVYIEKEGKLVLTDAKFKNDNHVLHVIKKIVAPIGRRIDESSPMV
                     DARLPNGSRVNAIIPPLAIDGPSITIRKFSEDPYKVEDLINFGTMSSKMAELLKYCVE
                     GRLNIVVSGGTGSGKTTTLNVLSSFIPDDERIVTIEDAAELQLSQEHVIRLETRPANV
                     EGRGQITIRDLVRNSLRMRPDRIIVGEVRSGEALDMLQAMNTGHDGSLTTGHANSPRD
                     MLSRLETMALMSGLNLPVKAIRDQISSAIDLIIQQSRLMDGSRKITYITEVQGMEGDV
                     IILQDIYRFEQKGLDSRGKVKGEFVSTGIMPKFIQKLKEKGINIPLDILRHTNNATV"
     gene            14289..15260
                     /locus_tag="PK733_06420"
     CDS             14289..15260
                     /locus_tag="PK733_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ10212.1"
                     /translation="MVEIVYVIATLIFLASWALTSAVLFLLFYREKPIDKLKHFDEEY
                     VFNEKLKSHKKNKISLLKLISNSIPYLKLNKKQTKKLEIELVRADLPITAEELLVIKI
                     FSSSAFAFLVYALSKNYITAVMLFIIIWNTPKIFIAYRKKERIKLFDSQLNEGITIIS
                     NSLKAGYSFLQAVAVVTEETKDPFSKEFKKLLIEMSLGIPEEGTFANLLSRMESEDLR
                     LTINAILIQKDVGGNLSEVLDNISDTIRERQKIKNELKTLTAQGKLSGVIVTLIPIFL
                     GLIIYVLNKEYILLLFTSTGGLVMVAISILNQILGLFVIRKIVNIDI"
     gene            15316..16230
                     /locus_tag="PK733_06425"
     CDS             15316..16230
                     /locus_tag="PK733_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ10213.1"
                     /translation="MLYIAVFVCFTSVFMFAYGVLYSLNYSKILVENRVELIKNLHDP
                     NREYIENTYLAESLLTHIYNVFCNFLMDVTPEYKLAVLNKKLEKAGLLINGTVEKWLY
                     AKNMIMLIAFILSSLLSYIIEPSIAKAFIIAIIIVLFANTCLNFYIAKRFEMRKKNII
                     KDLPYTLDLITVSVEAGASFDGAIAKVISNIRGELCDEFSKCLKEIKMGIQRKAALKN
                     MSERCDVKELSMLVNSLIQADELGVSLGRVLRIEAANLREHRKQMAREKAMKAPIKML
                     FPLIFFIFPSIFIIILGPAVIRIFSIFR"
     gene            16310..16642
                     /locus_tag="PK733_06430"
     CDS             16310..16642
                     /locus_tag="PK733_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOJ10214.1"
                     /translation="MVEILKYNSKVIKNVLIADTFIERFKGYMFLREPHYEAIIIKPC
                     NSIHTFFMKFPIDVLFVNNHMEIIKKIESLERGKIITPVKEAKMVIEGKAGLFKDYQT
                     GSKIVILY"
     gene            16980..17297
                     /locus_tag="PK733_06435"
     CDS             16980..17297
                     /locus_tag="PK733_06435"
                     /inference="COORDINATES: protein motif:HMM:NF039477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HOJ10215.1"
                     /translation="MYTKTLERKLSIKNSTGDSQYEIPIELEYYLLESHSDYYNGLEG
                     EKVYGIGILKKLDEACCEESAVKNFFCSSNEAKEIVEKLADNEVTPMSLTYILDDLLE
                     TDT"
     gene            17713..19008
                     /locus_tag="PK733_06440"
     CDS             17713..19008
                     /locus_tag="PK733_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOJ10216.1"
                     /translation="MNIHTVGVGESLWLISQRYNIPYQSIAAANAIPPPYNLVVGQSL
                     FIPQAMQTHVVRRGESLWSIARLYNSSVETIRSVNNIPESGIIYPGQVLVIPVQSKQY
                     GQIQVNAYIEPTGNTTVDLNNLEEEAAPHLTYLSIFSYRVLENGSLIPPQNDNELIAA
                     AKSRGIALLMVITNFRGGTFDTALADVILKDTGTQDVLISNILSTMKQKGFTGLNIDF
                     ERVPPEDRDLYTEFVKKVRDRIKPEGYNISVALAPKTSATQVGAWYEAHDYGGLGSVA
                     DFAILMTYEWGWSGGPPLPVAPINEVRKVLDYAVSVIPRNKIMMGIPLYGYDWVLPYN
                     PKNQFARRISPLQAVERALQFGSRIQYDTVAQSPFYRYYDANGTEHIVWFEDSRSIYA
                     KFRMVHEYNLLGISYWVLGSRFPQNWLILEDLFNVQKLI"
     gene            19486..20559
                     /locus_tag="PK733_06445"
     CDS             19486..20559
                     /locus_tag="PK733_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10217.1"
                     /translation="MGKFKVGLVGIGRGTAYGHIFANNPKTEVTALCDINEESLAASG
                     KSFELHDNQLFSSYDDFINADFDIAVIGTPIPDHTEQVIKALESKKHVLCEVTAANTI
                     EGCQQIVDAVKKSDRKYMLAENCNYMHFVMQWKKMVDEGKIGKIVYAEAEYVHEIRGR
                     VLDSSTNEKKWRAERAPIHYCSHSLGPILHLMDDYIVRGTCMGKDVNIIPNVGVGAID
                     YQVALFETSKGAVIKVLRSSVLSRKPPLCHYELYGTKGFIENGYDSYDNEGRTYFEGE
                     DETSKPQVWTSSDPDAPEAARLGGHGTSEFYIVRDFIESIENDTTPPIDIVKAMDMTV
                     PGIIAHEAAMKGNVWLDVPRLGL"
     gene            20645..>21817
                     /locus_tag="PK733_06450"
     CDS             20645..>21817
                     /locus_tag="PK733_06450"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ10218.1"
                     /translation="MLAGVGKRIITPSAGVDLAGFGRLDRKNIGVHDDLYVNALALQQ
                     DEKRTLIICADIIGFGFDLTQKIKKDINCKYGFNEDEILLSASHTHSGPQTLENMLVT
                     LGKWNEDYIGHFLSCVMSSVESALKSMEEVDIYLGKTQCNIGINRRLIKDGKANFQPN
                     EEGAADKSVTVLKLMKGDTTKAVLFSYACHPSTIGDDFVSADYPGVAKKVVEYDLGKG
                     AVALFMQECCGNIRVRTIKGNDFRGGTWKDVREFGIELGAAVSKLCSGENGEMKKLDK
                     NDLKISTAIMHMQLPTRGKPQKEGLEKVKNSGNIHEQLWAERMLNDYANLNDTVPFTI
                     QRIALNNDFSIIAMSGEVCVEYGLYLKNMDAGKTIISAAYSNGVSGYIPTEEMFEQG"
BASE COUNT         7911 a         3049 c         4529 g         6328 t
ORIGIN      
        1 acattacaag tgctatcggt ggtgccatag cagctgcaaa tggagctgac ttcctttgtt
       61 atgttactcc ggcggaacac cttaggcttc ctacccttga agacatgaaa gaaggaatca
      121 tagcatccaa aattgccgca catgcagcgg atctggcaaa agggattttt aatgcagaaa
      181 aatgggatta tgaaatggga actgcaagac aggaactgga ttgggaaaaa atgttttctc
      241 ttgcaataga tcctgaaaaa gccaagcgtt acagggcaga atctcttcca caacataagg
      301 attcatgtac aatgtgcggt aagatgtgcg cagtaaggaa tatgaataaa gttattaatg
      361 gcaaaaatgt aagtatattg gattaaaaaa ttaagagaat ggagagatga gttatgaaaa
      421 aggtacttac tattgcaggc tctgactcag gtggaggagc cggaatacag gctgacatta
      481 aaacgtttgc agctcacggt gtatttggca tgagtgtaat aactgcagtt acagctcaga
      541 acactcaaaa ggtaattgca gtgcaggata taaatccgga aattattggg aaacagatag
      601 atgctatttt tgaagatatt ggtgctgatg cagtaaaaat cggaatggtt tctcaaattg
      661 agagcattaa gataatcgct gaaaaattaa gagaatacga accccggaat attgttctgg
      721 atccggttat ggtatcaaag agcggctatg ctcttctaaa cccggaggcc attgatgttc
      781 ttattaatga attggtgcct ttggctactg ttataacccc aaatattccg gaggctgaaa
      841 atataattaa tgcaaagatt ggtacactta aagatgtaga gcaagctgca aaggcaatcc
      901 acatgttggg accggaaaat gtactggtga aagggggaca catgtcaggc gattcaactg
      961 acatcctata tgacggtgaa aatattatct gccttgaggc tgaaagaatt gatactaaaa
     1021 atacccatgg aacagggtgc actctttctt cagcaattgc atcaaacctt gcacttggtt
     1081 tctctatcaa agaatccgta gaaaaagcaa aatcatatat tactactgca ataaggcatg
     1141 ctctgcctat aggcagaggt gcagggccta caaaccattt ttatgaacta tacaggaaag
     1201 cgggggtatg agtatagata tgagtactaa tatgactgtt ggcaatagta aaaataaaag
     1261 catttgtgaa agaataatgg agcttttgga aaaggtaaag gcaataaaac cactggtaca
     1321 tcatataaca aactatgtta cagtaaatga ctgtgcaaac atagtgctgg caataggagg
     1381 atctcctata atggcagatg atattgagga agttgaagac atagtttctc tgtcttctgc
     1441 acttgtacta aacatgggaa cactaaacat aaggactatt gagtctatgc tggctgcagg
     1501 gaaaaaagca aatcagcttg ggataccggt agtgtttgat cctgtgggtg caggagcaac
     1561 accacttaga aacaaaactg cagaaaggat tataagtgaa ctaaagcttg cggttatcag
     1621 gggcaatatg tcggaaataa aatcaataag cgggctgggt tctaatacca agggtgtgga
     1681 tgcctcagaa aaagacattt tattatccgg tgacctgcaa tatggtaaaa atattgcaga
     1741 taagctatct tccaggcttg gctgtactgt agccataaca ggccccgttg atatagtgac
     1801 aaaagacggg aaaactttct ttatagaaaa tgggcataaa atgctttcaa tggtaacagg
     1861 tacagggtgc atgtgcactt ccctcattgg tgtgtattgt ggagttgcca gcaactatct
     1921 tgaagctgca gcagcaggga tccttactat gggaatggca ggtgaagcag cttatgaaaa
     1981 cattgaggaa ggaaacagtg caagcagcgg atatggcgga tatgataggc atggtggaag
     2041 tggaagcttc agagtcaaac ttatagattt tgtatctcag atcagagaga atgatattct
     2101 tgaaagggga agaatatatg aaaagtaagg aaaactttgt agattactca ttgtacctgg
     2161 ttactgaaag gcaaatgctt cgaaacataa gcatggaaga agctgtggaa caggccatcc
     2221 ttggaggtgt aactcttgtg cagttaagag aaaaagatgt tacaactctg gaattttata
     2281 atatagcagt aaaagttaaa aaaattacag ataaatatag cattcctcta ataataaatg
     2341 atcgattgga cattgcattg gcagtagacg cagcaggtct gcatgttggg cagagtgaca
     2401 tgcctgcaaa agttgcacgg aaaatacttg gggataataa aatacttggt gtttcagctg
     2461 ccactattaa tgaagcttta caggctgtag aagatggtgc tgattatatt ggaactggtg
     2521 cggtttttgc tactgatact aaaaaggacg caaaatatgt taatataagg caattgaaag
     2581 aaatcacagg taaaattagc attcctgttg ttgcaattgg aggaataaac gaggataata
     2641 tagatatact caagggtaca ggcataaagg gagttgctgt tgtttctgca ataatgggca
     2701 aggaaaacat tagaaaagca tctgaaaact tgaaaaagac ctgggaaacc tgcagaaagg
     2761 gtaggtgaca tatcattagc ggaaaaagca ttaacattaa tacaaaaagc ataaacacag
     2821 gtaacgggaa tactaatatt attggaaaaa acattaatga aagtgcaaaa aaaattaatg
     2881 tagttgatga aataactaaa ggtataataa aaggagctat attcgacctt gacggtacac
     2941 ttattgattc aatgcaggta tggggtaaga tagacttgga ttttttgggc aaaagaggta
     3001 ttcctgtacc ggatgatctg gaaatcaaaa taaaaaacct gactttcagt gaaactgcgt
     3061 tatactttaa agagaggttc aatatacctg atagtgcaca agacattatg gatgagtgga
     3121 atgacatggc ctataatgaa tatgcacata atataaaact taaaaccggg tctcgtgaat
     3181 ttctttcact gttgaaatta aagggtatca aggtaggact tgccactaca aactgtaaaa
     3241 agctattaga ggcaacttta aataaaaatg gaatatttga atattttgat gctatttcaa
     3301 cagcagatga agttggaaag ggtaaaaatt gcccagatat ttacctgctt acagcaaaaa
     3361 agcttgggat caatccggat gaatgcattg tgtttgagga tattctcccg gcagtgatgg
     3421 gtgcaaaggc agcaggtatg aaagttattg gtgtttatga tgagtgttcc aaacatgaaa
     3481 tagagcggat aaaagatagg gcagatggtt tcataattag ctttgaggaa ctaatgtaaa
     3541 tgcacttttc gaatatataa aacaactgta aagaaaaata aaaaaaggct attattactc
     3601 acctgtaaag gaggaatatt tatgaaaacg aaacaactta gaatgcttgt tattggagct
     3661 catcccgatg attgcgaatt taaagcagcc ggaactgcta ttaaatttcg acaattaggc
     3721 catgtagtta aatttgtatc agcaactaat ggtgatacag gtcattatga aataggagga
     3781 ggacagcttg ccaggattcg tgctgaagaa gtggaaaaat cctgttcaat tgcaggcctc
     3841 aaataccaaa taatggatat acataacaac ggtttggaag ctgatattca aacgcgcgaa
     3901 aggtttataa ctcttatacg ggaatttgtg ccggatataa tcattactca ccgtcctaat
     3961 gactatcatc cggatcacag acgtacttca cttcttgtac aggattcatc ctatgctata
     4021 attataccta atgtttgtcc tcttactccc gtaatgccta aagcaccggt gatactttat
     4081 atgcaggata atttcaaaag gccttatgag tttgtacctg atatagttgt ggatatagat
     4141 gatgtttttg atataaagac acagatgatt aactgccata aatcacaact ctatgaatgg
     4201 cttccctgga ctgaaaacca acttgaccag ataccgcagg gagaagaaga acgtatcaag
     4261 tggctaaaag aatatatgct tgtacgtgat ggcaaggtag ccaaccgatt caggaatcaa
     4321 attatagata agtatggtat tgaacacggc agcaatgtaa aaactgcaga agcatttgaa
     4381 atatgtgaat atggtgcacc tgctgaagaa gacatcttga atgcatttga ttctgggaaa
     4441 taatgcagat ttgctgtaaa aaatcggaat cggcggaaat tttagccctc gcctgtagag
     4501 aagaaaggat tattggttat ggatacagag gatgctttca aaaaaataaa tgaatatatt
     4561 aacaaagcaa aggaattaat taaaaaaaca gatttcgata ttagaaccag atatgaggac
     4621 atagataaaa atttggttaa agctcttaag atatccgcaa aattaaagta tgagcttgga
     4681 gcggcacaga gctatacata tttaggtcat ctaaaatgta aattatccag ctatgatgat
     4741 tctttagaat actatctaaa tgctttggaa atatatgaag agctaaatga taaagaagca
     4801 tccgcatcca tatacaatga tatcgggaat gtttatctac ttcaggagaa cttaaatcaa
     4861 gctttggaca acttcataaa agccggtgag ataagtcttt ccgttgattc cagaaatata
     4921 ttatgcaaag ctctgactaa ttgtgggaca acaagcttta aacttaaaaa tccagaggaa
     4981 gcgttgaatt attatatgaa agctttgcct atctgcacag aacttggtga aaaagaggaa
     5041 aagtgcagaa tattaagcaa tataggggca atatatgtgg aatttaagga atttaataaa
     5101 gcgatgaaat attttctgga ttcccttaaa attgaaacgg aattggattc aacctatggc
     5161 atagcctatg ctaactttag aatagctgaa acttattact tgatggatat ttatgaaatg
     5221 gcaattgctt ttaataataa ggctttaaag gctgctgatg aaatagcaaa caaacctctt
     5281 atatcatatt gctgtgtttt gttttccaat atatatgaaa agtcgggaga ttacaaaaat
     5341 gctcttagat actttaaaga ttacagcagg ataagagaag atgtattgga ggaagatgga
     5401 aaaaggaaaa ttgcagatat aaaagctaaa tttgaccttt atagaaaaga aaaagaaaat
     5461 gaaatatata aacttaaaaa tataaaactt aaagcagtta ataaaaaact aaaaaaggcc
     5521 tatgaaaatg ccgaatactt tgcgaatatt gattttttga caaaaattcc aaatagacgc
     5581 agtattcata agtacataaa tgatgatctt attaatatga gaacaccttt tacgattgtt
     5641 ttatgtgata ttgattgctt caaaatagta aatgacacct atggtcatga taccggagat
     5701 tatatactgg tggaaacttc cacgaggcta aactctcttt tagcagataa cagcagaatc
     5761 tgcaggtggg gcggtgaaga gtttttaata gtacttcctg gtaccactct ggaccagggt
     5821 atatccatag cagaaagctt aagacagtct ataagctcca caccatattt gttcaatgac
     5881 aaatcaataa acattactat gacttttgga gtgtgtgagc ataatgaagg tgattctttt
     5941 tatggcttaa ttaaaagggc agataatgca ctgtacaacg gaaaaaacag aggcaaaaac
     6001 tgctgtgtta gtggttgatc aaacagcctt gtttattatg tcatgggaca tatatcaagg
     6061 aacacacctc tctcaagtaa gatgcattct cttcacatta aggaatgtcc aagtacatta
     6121 tatactaaaa tatttaggaa aggattttga atggtaatga tagatatatt tgaggttaaa
     6181 agtatatatg aaatagaaaa ggttgaagaa ttggcaaata agatatggaa tgaacactat
     6241 gtaccgataa ttggaaaaga acaggtagat tacatgctgg caaagttcca gagcagccag
     6301 gctattaaag accaaattga agttggctgc agatatttca taactacgtt taatggcgaa
     6361 tatgcaggtt attttgctat tttacctgat aaatccaact gcaaaatgtt tctcagcaag
     6421 ctatatgtag atagaagctt tagggagaaa ggaattgctg caagggtagt atcatatata
     6481 gaagatattt gcaaagaatc aggtttggaa atgatatggc tgacagttaa taaatacaat
     6541 atgaactcaa tagccgcata cgaaaaaatg ggttttgtaa aagaagcccc gattgttcag
     6601 gatattggca atggttattt tatggatgac tataggatgg aaaagaaatt gtaatccgta
     6661 caaaatgcac gttcgttctt aacaggtatg cttaaaaggc tgggaatatt ctactagcag
     6721 acttttatcg acaaattttt tcaaattatt attgaatcta cttgaaaggc tactctatgt
     6781 caattacaat actagtatca gcaaaatatt gatttgacta aagataaaca aatatccact
     6841 taaggaaata gaagatctac tgtaagttaa acttatccga tatttgttga tttactaaag
     6901 aaaaattgtc tggtaatatt gatagtttta tatgatttaa gtaaaggtga gatttatgat
     6961 taataatatt aatctggaca ttagtagaat aaacactatt attaaaagaa ttatagaagg
     7021 aataagttcc agcagggagg aagtacttaa gatagttgat agcatacgga acgagcagga
     7081 aaatgtgaag ctggaactat gtaaaattaa aaacgatttg ataaatgttg ttgacgaggt
     7141 ggatgcactg gagaaacagg ataaatacgc gagacaatgg ctggcggaag tatcaaaaaa
     7201 ttttaatata tataatgaaa atgatatcaa gaatgcatac gaaaaagcac tggatcttag
     7261 ggtaaaactt ataacaaagg aaaacgaaga aaaagtacta aaagataaaa gaaacaacct
     7321 tgaaatgacc ttgaagcgac tagtagaaaa tattgatagc agcgagaaaa taattaacca
     7381 gataagtatt gcactagcat atctggaagg ggacattctt tccgcctttg atgatgcaga
     7441 taaaaattct gaaatgttta tcggtataaa aatattggaa gctcaggaaa atgagcggaa
     7501 aagaatagca agagatattc atgacggccc tgcacagcat atggccaatg ttgctatgca
     7561 agcagatatt tgcaaaatgg ctattcataa tgatttacag gaaggactca aggaattgga
     7621 agccttaaaa gcaagtgtaa aagaagcttt aaaggaagtc agaagtatta ttttcaactt
     7681 aaggccaatg tctctcgatg acctggggct aaatcagact attcaggaaa ctgtaaaaac
     7741 tatttctcaa gaatctaata taaatataca actaaaatta aaacctatac agggagaaat
     7801 agaaccaatt attcaagttg ccgtgtatcg tattattcag gaggttttta acaacattaa
     7861 aaagcattcc ggcgcgaaac atgtaacagt taagttggac tttggcacta agtatctaat
     7921 cctggctata tctgatgatg ggataggatt taatgtagaa gaaactttaa agagagttaa
     7981 aactagaggg acctgttatg ggctgatagg aatattggat agagtaaatc aattacaagg
     8041 agaaatcagg attaattctt cggaaggaaa tggaacggtt tatactgtta agctacctgt
     8101 taatagggag gtgattaagg atgagtaaaa acataataaa aatactgatt gcagatgacc
     8161 atgaccttat aagacagggc ttgagaagaa ttataagctt tgaagaggat ataaaaataa
     8221 ttggagaagc tgaaaatggc caaaaagtat tggagatatt gaagtttaat agacctgatg
     8281 tagtgctgct ggatttgaat atgcctataa tgaatggtat tgaagctcta agaaagatta
     8341 aaattcttgg tgatgatgtt agagttatta tgctcaccgt tgaaaatgat aaaaaaacta
     8401 tttatgaagt aataaaaatt ggagcagatg ggtatatgct taaagactct gttggtacag
     8461 aaattgttga tgctatcaga acagtatacc agggagagaa atatattgat aaatcgttga
     8521 tttctatatt ttttatggaa attagaggta ataaaagcac aactggtgca accaacactc
     8581 tggataattt gtcaaaaaga gaaattgaag tattgatgca tatttcaaag ggactcagta
     8641 ataaggaaat tggggcacaa ctatacatat ctgaaaaaac agtaaagaat tattcaacta
     8701 gcttatttag aaaactcaat gtaaatgacc gtgtacaagc tgcaattctc gcagtagaaa
     8761 atgatataga gcatttttac aaatctaagt tccaaatcta ggttacaaat ttaagtttaa
     8821 attagctaaa aataattaat gctgctttct tgccttgcat aaaagaatag ggacttaagt
     8881 ccctattctt ttatgactta tttaccttta aaatatgact tttggaaact actatactac
     8941 tttgtgcagg attataatta acttacggga aaaagcaaat aacaaattaa aggagggcta
     9001 tcatgttaaa gaatgctaat aactatgtat catcacttgt aaaacaggaa aaagggcaag
     9061 gtatggttga gtatgctctg ctggtaggac ttattgcagt tgtggttatt gcagtgctgg
     9121 tattgcttgg gcctgctatt gtagctaaat tccaggagat aatagatgca ctgtagtaat
     9181 attttgacaa tacccttcaa aacttgccaa tactttactt aaatgctcta atcatagatg
     9241 cggacattga ttagagcatt ttaaaaagag cgtattttca aagattacta acatatattt
     9301 ttatatattt cggcaggtgt gggagattga tatgaaaatg ctgaaaagag agttaaagaa
     9361 taagaaagga caatcattgg tagagtttgc aataatactt ccactccttc tattagtaat
     9421 tatggcaata attgagtttg gagtaatgct gaattcatat cttaccatac ataatgcagc
     9481 gagagaaggt gcaagggcag gaattgcagg aagttccagc actgagatac agaatttaat
     9541 tatatctgtg tctccagggt tggatgttaa agatctaata ataaacatat cacctgttga
     9601 taaaagtaga aaaccggggg atgcacttac tgtacagata aggtataact accatcttac
     9661 agttcccgtt ataagtagag tgttcgtaaa taatgtagtg attttaagtg cacaagtgtc
     9721 tatgagaatt gaatagtaag tggtggttgt aatgaaaaaa ttaaacaata ataagggaaa
     9781 cacagctatt attttgtgct tgctttttac tgccttgttg gggtttgctg cttttgtaac
     9841 agatattgga atagtttatg cagaaaaaat aaaattatat aatgcattgg atgctgcagc
     9901 tctagcagct gcccttgaat tacctgacaa tattgaaaag gcaaggaagg tagccgtgga
     9961 atatctcgaa aaaaatagtg tcgatccggg aagtactgta ataactattg gcagtgataa
    10021 aaagagtatt cagatagaag gagtaaaaaa tgtaaagcat ttgtttgcac ctatattaaa
    10081 aatttatagc agtaatataa atgcagttgt aatagccgca attggacctg caaaatcagt
    10141 aaaaggcggt atcaggccat ttgcagtcga aacttttgat tattcctatg gtagtttagt
    10201 aacccttaaa gcaggtgctg gggatggtta taaaggtaat tattatgctg ttgcgcttgg
    10261 cggtagcggg gcaaagattt tcaaagaaaa tgctctttat ggttataata gtacaatatc
    10321 gataggtgac ttaatatata cagaaacagg aaatatggca gacgctgcta atgctattaa
    10381 aaactatata aatgccgaac gcagcagctt tgatgatttc cccaaaagtt ctgtacgatt
    10441 atggactgtg cctttagtgg atactttgaa agtgaatggg acaggtcaaa tattggtggc
    10501 aggttttggg gaattttatg tagaggatgt gactacaaac tcagggaaaa tagaaattac
    10561 cgggcggttt attagatatg ttaccaactc agatgttgat atgactttaa atgataccgg
    10621 cgtatacggt gcaaaactgg taaaatagag gtgatagcgt gaaaggtatt ggacaaaaat
    10681 taatattaat ttcatttggc ttggcggtaa tagctgctgg agttgttttt gcatatttac
    10741 aatccttgaa agagtctgag gtacaaatta aggagacaac aattttagtt gcagctgata
    10801 caataccccc gggtacactt atcaccagac aaatgattaa acaaatccag attcctgata
    10861 actcaatttt aatagattat attgatgact cttctgaaat aataggtaaa tatactaaag
    10921 aaaccattct aaaaaatgaa ggttttcata tagataagct tattggtaaa gacgaaggag
    10981 agttacgttt aagaatagat agcacccata gagcagtttc cataaatgtt acaggggatt
    11041 cgggagtttc agatttaata aaaccaggag attctgtaga tattgttgtg ttttttgcag
    11101 aaataaaaga tggacaaaaa gttatcagac ctgagttatc aaaaataatt ctgcaaaata
    11161 tcaagctact ggctatagat aagcagctta atagagattg cactttagca gaaagcaaag
    11221 aaaatgaaga aataccagag aaatttctgg tgacactttc agtgacaaca actgaattgg
    11281 aaaagcttgt actggctgag aatatcggta ttttgaagct ggccttaagg ccgttaaata
    11341 atgatgacat ggatgaaaca aagggagcta cgtgggaaga attaacagca gaattaaaga
    11401 acaggggaga attttcgcag gaagataatt taaagagcaa cggcgaaaac agtaataaat
    11461 ttattaatta caaagtaaaa cctggagaca cattaagaaa aatcagtaga gaattttatg
    11521 gtgactggaa aaaatatacg ataattaaaa aagctaataa tatacaagat gaaaatttaa
    11581 ttataacagg gcaagttatc aaaattccgg aaatataacg aggcgataga tgtcccctac
    11641 ctctattgga tgtacatgtg ttgagaaaat acagtgcata gtgaaggaag tgaaaaagtg
    11701 gacaaaatca gaatacttat agcagatgat attgaagaaa caagaaatgt tataaataaa
    11761 atattaaaga tagataatga cttatttgat gtagtaggag aagctgtaaa tggagaagaa
    11821 gttcttcaac tcattcctga agtaaatccg gatatagtac taatggatat taatatgcct
    11881 gtaataaatg ggctggaagc aacagaaaaa ataacaaatg aatatcctgc ggttattgtt
    11941 attattatgt ccgtgcaagg agaaagtgaa tacttaagaa aggctatgtt ccatggagct
    12001 aaggagtata tcattaaacc cttccattac gatgaattgt ctgaaacgat taagtcaact
    12061 tatgaaaaaa ataaagaaag aaaattaaaa tttccgagta atcgggaaca aatgcgaaat
    12121 gcaaaagtta ttgcattctt cagttcaaag ggaggcgtag gaaagtcagt actggcactt
    12181 aacacttcca ttatcttaag cagagaagtt aataaacgaa tacttctagt agacatggat
    12241 ttactatttg gcgatatttc aatattgatt aataaattta atcagaagac tattcttgat
    12301 gtattggatg atagccagtt aaattcttat gaaaatataa agccatattt gtataaacat
    12361 aatgagaatc tggatatact ttttgcaccg gcaaaacctg aagcagcaga atatattagt
    12421 aaagatagca ttgataaaat gattaaagct tttcaaacga attatgatgt gataattgtt
    12481 gataccggta taaactttag tgataatact ctttacattt tagactgcgc gcaaacaatt
    12541 ttatatattg ctactgcgga aattgttaca ctaaaaaata ccaaattagg attgcagatt
    12601 atgaaatctc taggatatgg caaagataag gttaagcttg ttataaatag atttactgca
    12661 atttatggca taagcaaagc agaagtggaa gaggcctttg aaggcgacgt ttttgctttt
    12721 attcctgaag agggaaaaat agtatgcagt tcagttaata aggggcaacc gttttgtgat
    12781 aacgctaaat atcgtaaatc caaaattgtg aatgcctttg aattgatgtg cagaagtttg
    12841 ctcaattagg ataatgtgta aaatgtccct taccaaaaga caacttggag gtgatgtgtg
    12901 aaatgtccct tgcaaaaagg ctggaagata ttaatattga taaaaaaaac agcaggttta
    12961 aagaaaaaga agcagatcct tattttgacc tgaagatgaa aattcaaaat agggtcataa
    13021 aagaattaga tattgatttt aatgacatat cagagcaaaa tgaagattta aagaaaaaaa
    13081 tagatttaat tcttacgaag aatattgaac aagaatctct gaatatgact aataatcaaa
    13141 aaaagaaagt aaaggaagaa ttattggatg agataattgg atttggacct attacgggat
    13201 tgctttctga taacagaatt actgaaatta tggttaatgg acctgaccaa gtatatatag
    13261 aaaaggaagg caagctggta ctgacagatg cgaaatttaa aaatgataat catgttcttc
    13321 atgttattaa aaaaattgtt gcaccaattg gcaggagaat agacgagagt tctccaatgg
    13381 tagatgccag acttcccaat ggctcaaggg ttaatgctat tataccaccc ttggcaatag
    13441 atgggccatc tattactata cgtaaatttt ctgaagaccc ttataaagta gaggacctga
    13501 taaattttgg aactatgagt tcaaagatgg ctgaacttct aaaatactgc gtagaaggac
    13561 gtcttaatat tgtggtttca ggaggtaccg gaagcggaaa aacaaccaca ttgaatgttc
    13621 tttcctcatt catacctgat gatgaaagaa tagtgacaat cgaagatgca gctgaacttc
    13681 aactttctca ggagcatgtt atcaggctgg aaacaagacc tgctaatgtt gaaggcaggg
    13741 gacagatcac tataagagat ttggtcagaa atagtttgag aatgcgtcct gatcggatca
    13801 tcgtaggaga agtacgttcc ggagaagcat tagatatgct tcaggccatg aatacaggac
    13861 atgatggctc tcttactaca ggacatgcca actctccaag agatatgctg tcaagattgg
    13921 aaactatggc gcttatgtcc ggcttgaatt tacctgttaa agctatccgt gatcagatct
    13981 cctcagctat tgatttgatt attcagcaat ccagacttat ggatggaagc agaaaaatta
    14041 cctatataac agaagttcag ggaatggaag gagatgttat tatcctccag gatatataca
    14101 ggtttgaaca gaaagggctg gacagcaggg gaaaggtaaa gggggaattt gtatctacag
    14161 gcattatgcc aaagtttata cagaaactaa aagagaaagg cataaatata ccgctggata
    14221 ttttgcggca tacaaataat gcgaccgtgt gaatctggtt atcacatatt atagttaagc
    14281 tttagggggt ggttgagata gtatatgtta ttgctacatt aatattccta gcttcatggg
    14341 cattgacaag tgctgtcctg tttttacttt tttacaggga aaagcctatt gataagctaa
    14401 agcattttga cgaagagtat gtttttaatg agaaacttaa aagtcacaaa aagaacaaaa
    14461 taagcttatt aaaattaata tcaaattcaa ttccttattt aaaactaaat aagaaacaga
    14521 ctaaaaaact cgaaattgaa ttggtaaggg cggatttgcc tataacagca gaagagttgt
    14581 tggttataaa gatattttcg tcgtcagctt ttgcattttt agtctatgca ctctcaaaaa
    14641 actatattac tgcagtgatg ttatttatta taatctggaa tacccctaaa atttttatag
    14701 catatagaaa gaaggagaga attaagctat ttgacagtca acttaatgag ggtattacca
    14761 ttatttcaaa ttctcttaag gcgggatatt cttttctaca agcagtagca gtagtaacgg
    14821 aggaaacaaa ggatccgttc tcaaaagaat ttaaaaaact attgatagaa atgagtcttg
    14881 gcattccaga agaaggtaca tttgcaaacc tgctttctag aatggaatcg gaagatctca
    14941 ggcttacaat aaatgccatt ttgattcaaa aggatgttgg agggaattta tccgaggtat
    15001 tggataatat ttcggatact ataagagaac gtcaaaaaat aaaaaatgag ctaaaaacct
    15061 taacagccca aggcaaacta tccggcgtta ttgttacgct tattccaatt tttttaggtc
    15121 ttattattta tgtgttaaac aaggaatata ttcttctttt gtttaccagc accggtggat
    15181 tagtgatggt tgctatttct atccttaatc aaatattagg attatttgtg ataagaaaaa
    15241 ttgttaatat tgatatatga agacgatgta tttttttctc atattagatt tatgtatgca
    15301 gggaggagcg cagatttgtt atatattgca gtatttgttt gctttacatc agtatttatg
    15361 tttgcatatg gggtattgta cagtttaaat tatagcaaaa tacttgttga aaatagagta
    15421 gagcttatta aaaaccttca tgacccaaac cgggaatata ttgaaaatac ttacttagca
    15481 gaaagcttgc ttacacatat ttataatgtt ttttgtaatt tcctaatgga tgtgactccg
    15541 gaatataagc ttgctgtgct taataaaaag ttggaaaagg cagggctgtt aataaatgga
    15601 acagtagaaa aatggttata tgctaaaaat atgataatgt taattgcctt tattttatcc
    15661 agtttgttat catatataat tgaaccaagc atagcaaaag ctttcattat tgcaataatc
    15721 atagtactat tcgccaatac atgtcttaat ttttatattg caaaaagatt cgaaatgagg
    15781 aagaaaaata taataaagga tttgccatat accctggacc tgattactgt aagtgttgaa
    15841 gcaggcgcat catttgatgg tgccattgca aaggtcatta gtaatataag aggagaactt
    15901 tgtgatgagt tttcaaaatg ccttaaagaa ataaagatgg gaatacagcg aaaagcagct
    15961 cttaaaaaca tgagtgagcg ctgtgatgtt aaggaactct ccatgcttgt taattcactt
    16021 attcaagcag atgaattagg agtcagtctt gggagagtat taagaattga agctgcaaac
    16081 ttgagagaac acagaaagca aatggcaagg gaaaaggcta tgaaagcacc cattaagatg
    16141 ctatttccac taatattttt cattttcccg tcaatattta ttattatttt agggccagct
    16201 gtaataagaa tcttcagcat attcagataa tataaaaaag tatttaaaga gaattacgag
    16261 gcatatttgt gtatatttag gtgtttttat gtatatttga ggtgtattta tggtagaaat
    16321 attaaagtat aattcaaagg ttattaaaaa tgtattaatt gcagatactt ttatagaaag
    16381 attcaaaggt tatatgtttc ttagagagcc tcattatgag gctattatta ttaagccctg
    16441 caatagtatt cataccttct ttatgaaatt ccctattgat gtcttatttg ttaataatca
    16501 catggaaata attaaaaaga tagagagtct tgaacgggga aaaatcataa cgccggttaa
    16561 ggaagctaaa atggttatag aaggtaaagc aggattgttc aaagattatc aaacaggtag
    16621 taaaattgtt attctatact aaaacaagct aatacaactc atgatttgta attcctcatt
    16681 agttgtttca aatcgcaatt tacagagatt ttgacaaaaa gaataaaaaa agattaaaaa
    16741 acttaaaaat aatagaataa attgttatac tgttgaaaaa aatgccgata tacaaaatat
    16801 gcaataaatt aaagtaaata ggtcaagtta caaaaaggct aaatagtgag tatttatgcc
    16861 ataataatct aaacattata tgtataaaat aaaagataat acaagtattg taaaataaaa
    16921 taatataatg tataatagtt gtaaatttta caaaattata aatatagggg gcaaattaca
    16981 tgtatacaaa aactttggaa agaaaactgt caataaaaaa tagtactggt gattctcaat
    17041 atgagattcc catagagctt gaatactatt tacttgaaag tcacagtgat tattataatg
    17101 ggcttgaagg cgaaaaagtt tatgggatag ggatacttaa aaaactagat gaagcatgtt
    17161 gtgaagagag tgctgtaaaa aacttttttt gcagtagtaa tgaagcaaag gaaattgttg
    17221 aaaaactggc agacaatgaa gtaactccca tgtctcttac ttacatactg gatgacttgc
    17281 ttgagactga tacttagaac atcactctta gtgcttaaga ctcaagactt aggacttaag
    17341 acttaagtct taacactgaa atttaagact aagactaaga ctaagactta agccttagaa
    17401 cttgaaaatt ttaacttgat gatacctgca tatatctctt atggaaaata cataacatag
    17461 taaatgctgt ataggatttc gtaaatttta tgaagggcta tacagcataa ccttaaaatc
    17521 tattctttat actgctcttt ttgctatttc ttaagaccct ttaagagagt ttttttattt
    17581 gcatcttaat tcttatcttt gcattaatct tcataattaa aatatcactg gaaaaattca
    17641 attactttgg aactaaatat acatttaact aataaattaa taagagaact attatgtaaa
    17701 tgaggtgttt ttatgaatat tcatactgtg ggggttggcg aatcgctttg gctaatatcc
    17761 caaagataca atatacctta tcaaagtatt gcagcagcta atgcaatacc accaccgtac
    17821 aatcttgtag ttggacaaag cttgtttatc cctcaggcaa tgcagacgca tgttgtaaga
    17881 agaggtgaat ctttatggtc tatagcacga ttgtacaatt catctgtaga gaccatcaga
    17941 agtgtgaaca atattccgga atctggaata atttaccccg ggcaagtgct tgtaattcct
    18001 gtgcagtcca aacaatatgg gcaaatccag gttaatgctt atattgaacc tacagggaat
    18061 actacagttg atttaaataa tcttgaagaa gaagcagcac cccatctaac ttatcttagc
    18121 atttttagtt acagggttct ggaaaatggc agtttgatac ctcctcaaaa tgataatgaa
    18181 ctgatagcag cagcaaaaag cagaggaatc gctttattaa tggttataac aaacttccgc
    18241 ggcggaactt ttgatacagc tcttgctgat gttatattga aagatacagg cacacaggat
    18301 gtattaatat caaatatact ttctactatg aaacaaaagg gatttacagg acttaatatt
    18361 gactttgaaa gagttccacc tgaagatagg gatttgtata cggaatttgt gaaaaaggta
    18421 cgtgatagga tcaaacctga aggttacaat atctctgtag ctcttgcacc taaaacatca
    18481 gccacgcaag ttggagcctg gtatgaggcc cacgattatg gagggctggg ctctgtagcc
    18541 gactttgcga tattgatgac atacgaatgg ggttggtctg gaggaccacc tttaccagtt
    18601 gctccaatta atgaggtaag aaaagttttg gattatgcgg tttcggtaat tccaaggaat
    18661 aaaattatga tggggatacc cctttacgga tatgattggg tacttcctta caatcctaag
    18721 aatcaattcg caagacgtat aagtcccctt caggcagttg aaagagcact gcaatttgga
    18781 tcgcgtatac agtatgatac tgttgcccag tcaccgtttt acaggtatta tgatgctaat
    18841 ggtacagagc atattgtatg gtttgaagat tcaagaagca tatatgctaa attcaggatg
    18901 gttcatgaat ataacttact tggtataagt tattgggtcc ttggaagcag atttccgcaa
    18961 aattggctta tactagaaga cttatttaat gtacagaaat tgatataggt aaacaggggc
    19021 atttcccagc taaatgggga cgttccacaa aaacaaaaaa tgtccccata tatatgggcg
    19081 catttacttc ctatgataca aaatatggca gcaaacgatt ttccaagtat aaaatttgaa
    19141 caaagtttac ggattgttat gtttaaggta agggaaaatt cgtaattcac gacacggatg
    19201 tcgtgagccg tcacctgaat catggatgat gaatgtgacg ggtagaattt ttctgtagac
    19261 ttaaacgcta caatccgttg ctgaagtgaa tattatacat ggaaatcgct tcttgccata
    19321 tttcccctca aatatagaaa atgtagaaaa tgcaccgata aatataaatt gtattgcaaa
    19381 aagcttctcg aggtagtaaa attaatttat atatttatac aataaataaa ttaattgatt
    19441 aataacaaga gcataatgta caataagaca cgggaggttg ctaatatggg taaattcaag
    19501 gtaggcctcg taggtatagg cagaggcact gcatatggtc acatctttgc aaataatcca
    19561 aaaacagaag taacagcgct atgtgatatt aatgaagaaa gccttgcagc aagtggtaaa
    19621 agctttgaac tgcatgacaa tcaattattt agcagctatg atgacttcat aaatgcagat
    19681 ttcgatatag ctgttattgg aactccaata cctgaccaca ctgagcaggt tataaaggcg
    19741 ttggagagca aaaaacatgt attgtgtgaa gttacagcag cgaatactat tgaaggttgt
    19801 cagcagattg ttgatgctgt aaaaaaatct gataggaagt atatgcttgc agaaaattgc
    19861 aattacatgc attttgtaat gcagtggaaa aagatggtcg atgaaggtaa aataggcaaa
    19921 atcgtatatg ctgaagcaga atatgtacat gaaatccgag gacgtgtttt agattccagt
    19981 accaatgaga agaaatggcg tgctgaaaga gcacctattc actactgttc ccacagcttg
    20041 ggtccaatat tgcatttgat ggatgattac attgtcagag gtacctgcat gggtaaggat
    20101 gtaaatataa tacccaatgt aggagtaggg gcaattgatt atcaggtagc attgtttgag
    20161 acaagcaagg gagcagtaat aaaagtttta aggagttcag tcctttctag aaaacctcca
    20221 ttatgtcatt atgaattata tggcacaaag ggctttatag aaaatggtta tgatagctat
    20281 gataatgaag gccgtactta ttttgaaggt gaagatgaaa cttcaaaacc gcaggtttgg
    20341 acttcatcag atcctgatgc tcctgaagct gccaggctgg ggggacatgg tacatccgaa
    20401 ttctatattg taagagattt tatagaatca attgaaaatg atactacacc acctattgat
    20461 attgttaagg ctatggatat gaccgttcca ggaattatag cccatgaagc ggcgatgaag
    20521 ggcaatgttt ggctggatgt tccaagattg ggtttataat aggatagttt tgagtttata
    20581 atagaatagt tttgagtttt tgaatataaa aaaaataatt aatatattgg aggataatat
    20641 taatatgtta gctggcgtcg gtaaaagaat aattacaccc agtgctggtg ttgatttggc
    20701 aggatttgga cggttggaca gaaaaaatat tggcgtccat gatgatttgt atgttaatgc
    20761 ccttgcttta caacaagacg agaaacgtac actcattatc tgtgcagata ttataggatt
    20821 tggatttgat cttacacaga aaataaaaaa agatataaat tgcaaatatg gcttcaatga
    20881 ggatgaaata ttattaagtg catctcatac ccacagcggt ccacaaaccc ttgaaaacat
    20941 gctggttaca ttagggaaat ggaatgagga ttatattggg cattttctca gttgtgtgat
    21001 gtcatcagta gaatctgctc ttaagtccat ggaagaggta gatatttatt taggaaagac
    21061 acaatgcaat attggtataa atagaaggct tataaaggat ggtaaggcaa atttccagcc
    21121 taatgaggaa ggcgcagcag acaaaagtgt tacagtttta aagcttatga aaggtgacac
    21181 aacaaaagct gttcttttca gttatgcgtg ccacccaagc acaattggtg atgattttgt
    21241 aagtgcagat tacccgggag tagcaaagaa ggtagttgaa tatgatctgg gtaagggtgc
    21301 agttgctctc tttatgcagg aatgttgtgg aaatataagg gttaggacaa taaagggtaa
    21361 tgatttcaga ggcggaacat ggaaggatgt acgtgaattt ggaatcgaat tgggggcagc
    21421 agtttctaaa ttatgtagtg gggaaaatgg agaaatgaaa aaattagata aaaatgacct
    21481 taaaatatca acagcaatca tgcacatgca gctgcctaca agagggaaac cacagaagga
    21541 gggcctggaa aaggttaaga acagcggaaa tatccatgaa caactttggg ctgaaagaat
    21601 gttgaatgat tatgctaatc ttaacgatac tgtcccattt acaatacaaa gaattgcatt
    21661 aaacaatgat ttttctataa ttgccatgag tggtgaagta tgtgtagaat atggattgta
    21721 tctaaaaaat atggatgccg gtaaaactat tatttctgct gcatacagca atggtgtttc
    21781 gggttatata ccaacagaag agatgtttga acaaggc
//
LOCUS       DAOSDD010000050        21803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1047_length_21803_cov_4.849871, whole genome shotgun sequence.
ACCESSION   DAOSDD010000050 DAOSDD010000000
VERSION     DAOSDD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21803
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_21803_cov_4.849871"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PK733_06455"
     CDS             complement(<1..1005)
                     /locus_tag="PK733_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10219.1"
                     /translation="MDKIKVGIIGTGSISSVHINGYKALDNVELYAACDIDEEKVKKC
                     AEKNNIKHVFTDYNEMLKLKELDAVSVCTWNSEHASAAIAALKAGKHVLCEKPMAMNT
                     KQALEMEKAAKEAGKLLMIGFVRRYGNDCKVLKDFIDNGMMGDIYYSKATYLRRAGYP
                     GGWFGDSKRSGGGPLIDLGVHVIDLVRYLMGLPKAVAVTGVTFDKLGSRTNIKKRGGY
                     TSSDKSGANIFDVEDLAVAIIKFDNGAALTVETSFSLNTKTNSGNIELFGTKAGAKLD
                     PKLEFYTEQNDYLVDVTPAFNTELSFEGLFEGEIAHFIDCVANGTKCISPAEDGVELM
                     R"
     gene            complement(1063..1917)
                     /locus_tag="PK733_06460"
     CDS             complement(1063..1917)
                     /locus_tag="PK733_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ10220.1"
                     /translation="MADFKIGVIVDSFRISIKDAVKKAKEVGADGIQVYTTRGPMAPE
                     NLSAKERKEFLDYVTSNGLVISALCGDLGEGFADRENNPRRIEKSKRIMDLAKDLNTS
                     VVTTHIGVVPEDSNHPRWSILQEACEQLGEYGDNVGAYFAIETGPETSAVLKKFLDSL
                     SSRGVRVNLDPANLVMVTGDDPVQAVYNLKDYIVHTHAKDGIMLRKGNPEVIYGMIED
                     EIQQGAAFKEVPLGEGSVNFPEYLKALSSIGYNSFLTIEREVGENPEKDIRLAVDFLN
                     KNISLLKG"
     gene            complement(2183..3553)
                     /locus_tag="PK733_06465"
     CDS             complement(2183..3553)
                     /locus_tag="PK733_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ10221.1"
                     /translation="MNSLDLIGMAFKNLWRRKVRTILTVLGVIVGTASIIVMLSLGIG
                     MNESFKQQVSMMGSLNIINIDTYYYPEMEEKAVSTRSSRQQNQTLDDKILAKIAAIDG
                     VEAVTPLLTTYIKFESGKYISHMQLVGINAGSMDAFGFKLQQGRILNENDSNSVVFGS
                     NIKDTFYDPKSRNWYNREQPNIDVFNDKITISFDMGWSDKPNNTGNRSLRDRNKGQNI
                     EVVGLLEPGQNEKDYSAYMNVEHLKKLIQANSRNNQNSGRIQYGVSNNNKSGYERAMV
                     KVKDLKDVERIQAAIKEMGFGAYSLTDILRQMLDATKTIRTILGAIGAVALFIAAIGI
                     TNTMVMSIYERTREIGIMKVLGCVLGDIRRVFLLESAMIGFLGGLAGIGFSYLTSFLL
                     NKAGISFLDTGWSMQGAPPSHISIIPLWLALVAVGFASIVGIVSGAYPATRAMRLSPL
                     EAIRNE"
     gene            complement(3546..6173)
                     /locus_tag="PK733_06470"
     CDS             complement(3546..6173)
                     /locus_tag="PK733_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOJ10222.1"
                     /translation="MKLKVQIDRENNMTDLNKNDGKNPDYTADSRISSRLNNNRSRFR
                     LGSRGIRVILACLLVALQMMSLIAVPGIAIKEVKAGDNDVTLEIEVPEVVQKGERFQV
                     TAKVTNNSGADIDDLILCIVPYDSLVPAGSDWNIPVKGTIKKNSTYPINVNMSYNGAD
                     DSFKLGMYCKYNNDIGTTLTVQKFIIANTKPVDNTPTDTTKYKPDIKVKSGLQMPVVN
                     AGDDVSLTLPIVNAGATEAKDIIVSIDTSDVSSLPFTFNQVNLSSTLKSLVSNKEESV
                     VFNIGVKSDIAGGIYPVKLNYQYQNPYRDSFSSSETIYIKVVNNKVPPKIILERFQTY
                     SNNGEYPKPGSIASLTLKFKNQGSISAKDVKVSIKGLSNDGISVVKSADTKYISEIEG
                     QKTQDVDYFLYISDKFKGNNATLQANVEYKDSMGKTYSEEYQFFVMVQETKEDIDDSK
                     PELILKDLVFPKEKVEAGKDFNIGFTLDNMGDDTAHNVKVTLSCEDGILPISAGTVVV
                     DALNKGESKNLSFNLYANKTAITKNYLVTINVEYEDKKDDKSDSAGSKTEKYTLTRYA
                     GVYVEKKEDEKDKDVKTVPKIIISMYTFEPEEVKAGENFKLKLSFLNTSKIVPIRNVK
                     VTFSAKEGVFIPTSSSNTFFIENMGIQEAVARDVELFTKSDAPAKSHILNLNFEYEDG
                     NGNQYTAAEEISIPVKQEQRLSMGQLQMPPDTMEGQPIPIFMDFFNMGKSTLFNLMVS
                     IEGEGLKSENANYFVGNFEPGRSDYFESMVTPTATGEIAGNVLFSYEDESGKKNEIRK
                     EIKLNVRAMEMPPMPSENGEPGKEFPGEMMPEGPQQKKLLTPLNIGIAAGALIIIVII
                     LIIILRKRRIRKAGMMFDE"
     gene            complement(6151..6846)
                     /locus_tag="PK733_06475"
     CDS             complement(6151..6846)
                     /locus_tag="PK733_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10223.1"
                     /translation="MESIIILQDVSKIYRIGNEKVIALDNVSLNISKGEICCFLGTSG
                     SGKSTLLNLMAGLEKPTKGRIIIKDNEMQKLNERKLTKFRQDYIGFVFQSYNLIPTLT
                     ALENVSLPLTFRGIPKAKRDKRARKMLKDVGLETHLKHKPTQMSGGQQQRVGIARAFV
                     GKPEIVFADELTGNLDTKTTKEVMDLITTMARENNQTLIIVTHDIEIAGYADRIFYIR
                     DGRIDKIEAKSAN"
     gene            complement(7105..7620)
                     /locus_tag="PK733_06480"
     CDS             complement(7105..7620)
                     /locus_tag="PK733_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOJ10224.1"
                     /translation="MGKLIIIEGDITRVDVEAIVNAANNTLLGGGGVDGAIHRAAGPG
                     LLEECRNLGGCETSEAKITQGYDLPAGYVIHTVGPVWSGGNNGEDELLAKCYRNSLKL
                     ASQKGVKTIAFPSISTGAYGFPVNRAARIAVNEIYNFLESNNSISRVFIVCFDNNTYE
                     SYMNAKSEIYG"
     gene            complement(7802..8545)
                     /locus_tag="PK733_06485"
     CDS             complement(7802..8545)
                     /locus_tag="PK733_06485"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOJ10225.1"
                     /translation="MTIGLQSVAIQSNEEKKNEIRSGRIWEIDFFRGVALILMIYFHI
                     VFNLKDIFGYNIDYTKGFNALTGRTAGTLFIVLSGISCTLSRNNIKRGLKILGLAMLI
                     TVATHLYNVDMGIKFGVLHFLGVSILLFPLLSRLNTNLLLALGTIIMILGNLIKHITL
                     DFDYFFIFGITSNKFVSADYYPLIPWLGIFIYGIVLGKVLYKDRNSVFKFTLKDNIIS
                     WVGRHTLIIYMIHQPIIIYTIKLIERIKG"
     gene            8706..9506
                     /locus_tag="PK733_06490"
     CDS             8706..9506
                     /locus_tag="PK733_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HOJ10226.1"
                     /translation="MIIIGEKINSTIKSVRDAIEKHDSLFIQDLAEKQCKAGANYIDV
                     NAGMFYDNEPENLEWLVKTVQEGTDAPMAFDSPNPAALEKALKVNKNGKPIINSITDE
                     KERYESILPLILEYNTSVIALCMDDSGMPETIEDRFLVAERLIDKLTSKGVNPCDIYI
                     DPMVRPIGTGSHYGITAIETIRIVKSKYPEVHIACGLSNISFGLPGRKVFNQAFLIAA
                     MTAGLDGAILDPLDKKLMTFLYATEALLGQDEFCMNFLTKYREGVLDI"
     gene            9509..10792
                     /locus_tag="PK733_06495"
     CDS             9509..10792
                     /locus_tag="PK733_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ10227.1"
                     /translation="MKVLGIVTEYNPFHNGHLYHLLKSREVTSADYTVAVMSGNFVQR
                     GEPAIVNKWARSSMALSSGVDLVIELPVIYATASAEFFSWGAVKLLDSLGITDCICFG
                     SESGEMEPLNLIAEILANEPEEYKKLLKTCLNMGLSFPAARAKALKEYDNSLAADQIL
                     RYSNNILAIEYLKALKKLNSRIEPFTIKRKSNLYTEENLTGEFSSAKAIRKCVSEGVD
                     NSILLDNCLIKNTIPSESAEILNTEFMNGRGPVLFNDFENIILASLRKNSLDWIKLYP
                     YITEGLENRIKNAAKSSGSLQELINNICSKRYTITRVQRIITSFLLNITQQEFERFNI
                     YGGPQYIRVLGFNEKGTQLLSEINKNACLPVITKTADFTRTCNILLRRMLEIEALATD
                     IYVLGYCNPDFRTGGQEYTQNPVRMLNKGSSAVNK"
     gene            10942..12234
                     /locus_tag="PK733_06500"
     CDS             10942..12234
                     /locus_tag="PK733_06500"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOJ10228.1"
                     /translation="MDNDFVIGDIEFNIDRAIQAVNAYSHSTGIRCIIIDSKGKTLYE
                     KCVDMTGCLFCKKAEDLLGEHQSCTNAHLYGSYQAERFGGKYVFFCPMGLVHWVSPIT
                     KNNILKGAVLAGPVQMVEPEEFLIEDILRKNNIKEEDINAIKESVHDIPVIKTEVVNS
                     MSEILYIVAAYISNVQPSKYLEEIEALEQQSDISEYIHYIKTFGGDSSELNSYPIEKE
                     KELLMLISIGDKAGSQKILNEIFGHIFFSTGGNFEVIKARVLELTVLLSRAALEGGAD
                     AEQIFGLNYIYLNEIHSFSTVEELTYWLSNIMMRFTDCVFTLAGVKHVDVIYRAIEYI
                     KRSYMKKVTLEEVASYVYLSPAYFSKIFKEEMKTNFNSYLNHIRIEMAKKLLLDDTIS
                     LVDVSNVVGYEDQSYFSKVFKRMVGVSPGKYREARGKI"
     gene            12354..12992
                     /locus_tag="PK733_06505"
     CDS             12354..12992
                     /locus_tag="PK733_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ10229.1"
                     /translation="MSVLNEISQNLQLGKAKNVKELVQRAVDEGIDVKLILEEGLLSG
                     MSIIGEKFKNNEVYVPDVLIAARAMNAGTEILKPLLASSGIKAKGKVVIGTVKGDLHD
                     IGKNLVRMMMEGQGLEVIDLGIDVSAEKFVKSAMENEAGIIACSALLTTTMGEMKNVV
                     EEAKKVGIRDNITIMVGGAPVNENFCKSIGADIYTPDAASAAEEAAKVFAAK"
     gene            complement(13207..14874)
                     /locus_tag="PK733_06510"
     CDS             complement(13207..14874)
                     /locus_tag="PK733_06510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOJ10230.1"
                     /translation="MAKKKKKMKVIPLGGVNEIGKNITVFECEDDIIIVDCGLTFPED
                     EMLGIDLVIPDVSYLIKNKEKIRGIVLTHGHEDHIGALPYVLKQINAPVYGTRLTLGL
                     LEYKLEEHGMLDQVVLQRVEQGQSIELGCFKVEFIRSTHSIADSVALAIFTPLGIIVH
                     TGDFKIDYTPIEGKPMDFARLAELGSKGVLLLMCDSTNIEREGYTMSERTVGNTFEEI
                     FMKAKGRIIIATFASNVHRIQQVINASVKFDKKVAICGRSMTNVVDVATKLGYLTAPE
                     GTIIDVDDIDRYPIDKTVVITTGTQGEPMSALSRMAASEHKKVEIVPGDLVIISASPI
                     PGNEKLVFKVINELFKRGANVIYESLADIHVSGHACQEEIKLIHTLIRPKFFMPVHGE
                     YRHLKQHANLAHELGMPMENIFIPEIGRVLELSAEEAKLNSSVPSGRVFVDGLGVGDV
                     GSIVLRDRKHLSQDGLIVIVITTERETGNLIAGPDIISRGFVYVRESEDLMEEARLVT
                     RNALLKCDDKKKTDWSTVKNIIKDDLRNFLYEKTKRKPMILPIIMEI"
     gene            complement(15207..16409)
                     /locus_tag="PK733_06515"
     CDS             complement(15207..16409)
                     /locus_tag="PK733_06515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ10231.1"
                     /translation="MKILMLSWEYPPRIVGGIARVVYNLAQKLGEQGNEVHIVTWKEK
                     NDVNEAEDEKDEKVFVHRTNIYDIAPGNFIDWVLHMNFSLLERSIGLFNEIGEFDIIH
                     AHDWLTAFTARALKHSYKAPLVSTIHATEAGRNSGIHNEVQSYINSVEWWLTYESWRV
                     IVNSRYMFYEVKNNFQLPEDKIRIIPNGVNLKKFDNFSRDMQFRNNYAMSNEKIVFFV
                     GRIVNEKGVHILIDAIPKILCHYYDVKFVIAGKGPELDYLHGKVCAMNIEHKVYFTGY
                     ISEEVLGKLYKCADIAVFPSLYEPFGIVALEGIMANVPIVVSDAGGLGEIVEHGVDGL
                     KFYSGNPSSLADCILEILFNPEKAEIMKKKALDKVRNIYNWDSIADQTFEVYREITVS
                     SNKVIKIN"
     gene            16646..17335
                     /locus_tag="PK733_06520"
     CDS             16646..17335
                     /locus_tag="PK733_06520"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOJ10232.1"
                     /translation="MPVLEEHSKSQYELPESYEDNNIAIMAVDAHTLYAYWDISNNKM
                     QNLINNIGSMVWENSVPALKITNLTKNSSFFVRINGFSHDWYINIHDSGCIFVAELGR
                     KITDDYFISFATSNHISLPDTSISFNNTACFVNCKYAMESAFSGDDNIPLEYGINVNG
                     NLRRITALMFNEEFGKLPDFEEIYGSCSSSISSMFYCEQERVSSLENFNYEKKKILSL
                     SSFEIIKKDKK"
     gene            17480..19069
                     /locus_tag="PK733_06525"
     CDS             17480..19069
                     /locus_tag="PK733_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOJ10233.1"
                     /translation="MAKGYVAFVLHAHLPFIHHPEQDDFLEERWLFEAISETYIPLIT
                     VFNRLLDEGLDYRVTVSFTPPLLSMLSNNLLQNRYIRYLEKLIELSEKEIKRTEGQID
                     FNRLARMYNDKYKNDLYIFRDKYGKDLVNAFKNLYDSGKFEFITSAATHGYLPLLAIN
                     PESINAQIALGVKSFQRFFGFKPKGMWLPECGYVHAIEPVLKKYGVEYFFSEAHGILF
                     ADPRPVFGTFSPIITPKGMVVFGRDTDSSKQVWSSKEGYPGDFDYREFYRDIGYDLDY
                     EYIKEYISADGQRIDTGIKYYRITGNTENKQPYNPGWAQNKADLHAFDFMYNRQKQIE
                     YLSDIMDKPPIIVCPYDAELFGHWWYEGPYWIYSLFKKMDVPGNPIKLTTPGEYINGN
                     PIMQVSSPCPSSWGYNGYNEVWLNKTNDWIYPHLHKAAERMTELANENKNATGLKQDA
                     LNQAARELLLAQSSDWAFIMKTGTMVRYAEKRVKDHIGRFLKLYHDIKEGVIDENWLR
                     DIEYKDDIFPNMDYSLFTSGG"
     gene            19391..19714
                     /locus_tag="PK733_06530"
     CDS             19391..19714
                     /locus_tag="PK733_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10234.1"
                     /translation="MENEKLFEFLTIMHSEMKEGFSKIDERFSEIDERFSEIDERFNE
                     IDERFSEVNNRLTKIENTVIKIENEHGKKLQVLFDGYRQHSDQLERIEKEVTKHDEFI
                     LKRVK"
     gene            19855..19928
                     /locus_tag="PK733_06535"
     tRNA            19855..19928
                     /locus_tag="PK733_06535"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19887..19889,aa:Gln,seq:ctg)
     gene            complement(20019..20894)
                     /locus_tag="PK733_06540"
     CDS             complement(20019..20894)
                     /locus_tag="PK733_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10235.1"
                     /translation="MRAFKEYVNLRDEGLPFNIWISERSDIRSYIDSQKQQHNDYNDL
                     WHEFTEIVYVICGAVDQRINGNTYRMKKGDMAYINGLDLHSLYLNPNIYIKILIMQIP
                     PSFISLYYREHWNHKIISNYFPFNGSDEVNIEIQRCMANIIEEFINKNSAYGYYLASH
                     MYKLLGIIYRNTQFVDSRAEEYMKQKLLLEKISPAIDYIFKNYNNSISINDVSSLTGM
                     CNQHFCKCFKKATKYTFTQYLNDVRVLYAQELLLTTNMNITEIAFSCGFSSVSYFNRV
                     FKKKSGISPLEFRRK"
     gene            21363..21647
                     /locus_tag="PK733_06545"
     CDS             21363..21647
                     /locus_tag="PK733_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10236.1"
                     /translation="MKTSDLLISSVNITEDEYHYKGQFVLSSEHKSLIVDLAVLYDDN
                     EINNIKEEFNLQESNSEIRESLLNRLVIKAGISSKIVQGEDYFELKAKRT"
BASE COUNT         6910 a         4099 c         3550 g         7244 t
ORIGIN      
        1 tctcataagt tctacgccat cttctgcagg acttatgcat ttagtaccat tggcgacgca
       61 atctataaag tgggcaattt ctccctcaaa cagcccttca aaacttaatt cagtattgaa
      121 agccggagta acatccacca gataatcatt ttgttcagta tagaactcca gtttaggatc
      181 aagttttgct cctgcttttg taccaaaaag ctctatattg cctgaattgg ttttggtatt
      241 caagctgaaa ctggtttcta cagttaatgc tgctccgtta tcaaatttaa ttatggcaac
      301 agccaggtct tcaacatcaa aaatattggc cccgctttta tctgatgaag tatagccgcc
      361 acgcttttta atattcgttc ttgatccaag cttgtcaaag gttactcctg ttactgcaac
      421 tgcttttggc aagcccataa gataacgcac aaggtctata acatgcacgc ccaaatcaat
      481 aagaggtcca ccgcctgaac gtttgctgtc tccgaaccag cccccagggt aacctgctct
      541 tctaagataa gtcgcttttg aatagtatat atcacccatc attccgttat ctatgaagtc
      601 ttttagtact ttgcaatcat taccatatct tcttacaaac cctatcatta gaagctttcc
      661 ggcttcctta gcagctttct ccatttctag agcctgcttt gtattcattg ccataggttt
      721 ttcacataat acatgctttc ctgcctttaa tgcggcaatt gctgcagaag catgttcact
      781 gttccatgtg caaacactca cagcatccag ttcttttagt ttaagcattt cattataatc
      841 agtaaaaaca tgcttgatat tgtttttctc cgcacatttc tttacctttt cttcatctat
      901 atcgcaagca gcataaagtt caacattgtc aagcgcttta tatccattaa tatgtacact
      961 gctaatacta ccggtaccta taattccaac cttgatcttg tccattataa aaatcctcca
     1021 gtattatatg atatcatttg tactgttatt cataacactt ctttagcctt tgagtaaact
     1081 gatattttta ttcaaaaaat caactgccag tctgatatcc ttctcaggat tttctccaac
     1141 ttctctttct atagtaagga aactattata tcctatagaa gataaagctt ttagatattc
     1201 cggaaagttc acacttcctt caccaagagg aacttcttta aaagcagcac cctgttgaat
     1261 ttcatcttct atcattccat aaataacttc aggattacct tttctcagca ttataccatc
     1321 tttggcatga gtatgtacta tataatcttt aaggttataa acagcctgca cagggtcatc
     1381 acctgttacc ataaccaggt ttgcgggatc gagattaact cttacacccc ttgaactgag
     1441 actatcaaga aattttttca aaacagctga tgtttcaggc cctgtttcga tagcaaaata
     1501 tgctcccaca ttgtctccat attcgcctaa ttgttcacag gcttcctgta gtatggacca
     1561 tctgggatga ttggagtctt caggtactac gcctatgtga gtagtaacta cactggtgtt
     1621 taagtctttt gcaaggtcca taattctttt tgatttttca atacggcgtg ggttgttttc
     1681 cctgtctgca aatccttcgc ccagatcccc gcaaagtgcg gatattacca atccatttga
     1741 tgttacatag tccaggaatt cttttctttc ttttgcacta agattttccg gagccatagg
     1801 tcctcttgtt gtataaacct gtattccgtc agcaccaact tctttggctt tttttacagc
     1861 atctttaatg ctaatcctaa atgaatcaac aattactcca attttaaaat ctgccactta
     1921 agaccacctt acctttcatt aacaaattag tttacattaa ctttattgaa tattagctac
     1981 taatcaacta ctaatggaaa aaacaatata tatctttact catattatat ttttatagca
     2041 caaattgtca agacttaatg gaagatatgg atgtattcat caaaactgaa tattcgggag
     2101 gaaatttctt cctattatac aatatatata gaaaatgcgc ccataaatat acggacgcat
     2161 ttttctccta ctcgttttca ttctactcgt tacgtatggc ttccagagga ctcaatctca
     2221 tagctcttgt tgcaggatat gctccggaaa ctattcctac aattgaagca aagccaacag
     2281 caaccaatgc cagccataac ggaattattg aaatatgaga tggtggtgca ccctgcatgc
     2341 tccatccggt atcgagaaag cttataccag ccttgtttaa gagaaaggag gtaagatagc
     2401 tgaatcctat acctgccaat cccccaagaa aaccgatcat tgctgattcc agcaaaaata
     2461 cccttcttat atcaccaagt acacatccaa gtactttcat aattccgatt tctcttgttc
     2521 tttcataaat agacataacc attgtattgg ttattcctat agctgctatg aacaaagcaa
     2581 cagcaccaat agcaccaagt attgtacgta tggtttttgt tgcatctagc atctgtctca
     2641 atatatcagt cagactgtat gcaccaaaac ccatttcctt tatagctgcc tggattcgtt
     2701 caacatcctt taaatcctta actttaacca ttgctctttc ataaccactc ttgttattat
     2761 tagaaacacc gtattgtatt cttcctgaat tttgattatt tctgctattt gcctgtatta
     2821 gcttcttcaa gtgctcaaca ttcatatatg cagagtaatc tttttcattc tgtcccggtt
     2881 ccagtaaacc tacaacttca atattctggc ccttatttct gtcacgcaaa cttctgtttc
     2941 ctgtattgtt gggtttatcg ctccatccca tatcaaagga aatagttatt ttgtcattga
     3001 aaacatcaat attaggctgc tctctgttat accagttacg agactttgga tcataaaatg
     3061 tatcttttat gttgcttcca aaaactactg aattactatc attttcatta agaatacggc
     3121 cctgctgcag cttaaaaccg aaagcatcca ttgaacccgc atttataccc accaactgca
     3181 tatgagaaat atatttcccg gattcgaatt taatgtatgt ggtaagaaga ggagtaactg
     3241 cttctactcc atctatcgct gcaattttgg caagtatttt atcatccagg gtttggtttt
     3301 gctgtctgct tgatctggta gaaaccgcct tttcttccat ttcagggtaa taataagtgt
     3361 cgatattaat aatattaaga ctccccatca tgcttacctg ttgtttgaag ctctcattca
     3421 tacctatacc aagtgatagc ataacaatga tagatgctgt gcctacaata acacccagta
     3481 ctgtaagtat tgtccttacc tttctccgcc atagattttt aaaggccatt ccaataaggt
     3541 ccagactatt catcgaacat catgcccgcc tttcttatcc gtctctttct gagtataatt
     3601 attaaaatga ttacaataat tatgagagca ccagctgcta taccaatgtt taaaggagtt
     3661 agaagtttct tctgctgcgg tccctccggc atcatttcac ctggaaattc ctttcccggc
     3721 tcaccgtttt cggaaggcat tggcggcatc tccattgccc ttacattaag tttaatctct
     3781 ttccttattt cattcttttt tcctgattcg tcttcatatg aaaacaatac attgcctgca
     3841 atttctcctg tagctgttgg agtaaccatg ctttcaaaat agtcgcttct tcctggttca
     3901 aaattaccta caaagtaatt tgcattttca ctttttaggc cttctccctc aatacttacc
     3961 ataagattga agagggtgct tttacccata ttaaagaaat ccataaatat tggaatcggc
     4021 tggccctcca tagtatcagg gggcatctgc aattgtccca tagaaagtct ttgctcctgc
     4081 ttgacaggta tacttatttc ttctgctgca gtgtattggt taccatttcc atcctcatat
     4141 tcaaaattaa ggtttagtat atgggacttg gcaggagcat cggatttagt aaacagttca
     4201 acatcccttg caactgcttc ttgtattccc atgttttcga tgaagaatgt attgctgctg
     4261 ctggttggta tgaacacgcc ttccttggca gaaaatgtta ctttaacgtt tctaatgggt
     4321 actattttgc ttgtattaag gaaagacagc ttaagcttaa aattctcacc ggctttaacc
     4381 tcttctggtt cgaatgtata catactaatt attatttttg gtactgtctt tacatcctta
     4441 tccttttcat cttccttctt ctcaacatat acacctgcat atctggtaag agtgtacttc
     4501 tctgttttac tgcctgcact atctgattta tcatcttttt tatcttcata ttctacatta
     4561 attgtaacta aatagttttt agttattgca gttttgtttg catataaatt aaagctcaaa
     4621 ttttttgatt cccccttgtt aagggcatca acaactacag taccggcaga tatgggcagt
     4681 ataccatctt cacagctaag agttactttt acattatgag cagtatcatc acccatattg
     4741 tctaaagtaa aacctatatt aaaatctttt cctgcttcaa ccttctcttt tggaaagact
     4801 aaatccttca atattaactc aggttttgaa tcatctatat cctctttagt ttcttgaacc
     4861 ataacaaaaa actgatattc ttcggaatag gttttcccca tactgtcttt gtattctaca
     4921 ttagcttgaa gagtggcatt atttccctta aatttatctg atatataaag gaaataatca
     4981 acatcctggg ttttctggcc ttcaatttca gatatatact tggtatcggc agattttact
     5041 actgatatcc catcattact taagccttta attgatactt tgacatcttt tgctgaaata
     5101 ctcccttggt ttttaaactt caaagtaagg cttgctatac tgcctggctt tggatattca
     5161 ccattattac tgtaagtttg gaacctctcc aatataattt taggaggaac cttgttgttt
     5221 acaactttta tatatatagt ctcacttgat gagaaactgt ctctataagg attctggtac
     5281 tggtaattta gttttacagg ataaattcct ccagctatat cagacttgac tccaatatta
     5341 aatacaacac tttcctcttt attggacaca aggctcttta aagttgaaga gagattaacc
     5401 tggttaaaag taaaaggcag tgaactaaca tcactggtgt caatagacac aataatatct
     5461 tttgcctcgg tagctcctgc attaactata ggcaatgtaa gtgatacatc atctcctgca
     5521 ttaaccacag gcatttgcaa accagacttt actttaatat caggtttgta ttttgtggtg
     5581 tcggttggtg tattatcaac gggttttgta tttgctataa taaacttctg aacagtaagt
     5641 gtagtcccga tatcattatt atacttacaa tacataccta acttaaaact atcatcagct
     5701 ccattataag acatattaac atttattgga tatgtactat tcttttttat agttcccttt
     5761 acaggtatat tccagtcgga acctgccgga acaagcgaat cataaggaac gatacataaa
     5821 attaaatcat caatgtcagc accggaatta tttgtaacct tagctgttac ctgaaatctt
     5881 tctccttttt gaactacttc cggtacttca atttctaatg taacatcatt atctcctgct
     5941 tttacttctt taattgcaat acctggaact gcaattaacg acatcatctg caacgcaacc
     6001 agcaagcagg caagaataac tctaatacct ctgctgccta atctaaacct tgatctgttg
     6061 ttatttaatc tgctgcttat tctactatcc gctgtataat ccggattttt accatcattt
     6121 ttgttcaggt ctgtcatatt gttctcccta tcaatttgca cttttagctt caattttatc
     6181 aatccttcca tctctaatat aaaatattct atccgcgtac cctgcaattt caatatcatg
     6241 ggtaacgatt atcagggttt ggttgttctc tcttgccata gtagttataa ggtccatgac
     6301 ttctttagtt gttttggtgt caagattgcc tgtaagctca tcggcaaaaa cgatctccgg
     6361 cttgcccaca aatgctcttg ctataccaac cctttgctgc tgcccgccgc tcatttgtgt
     6421 tggtttatgc ttcaagtgag tttccagccc tacatccttc aacatttttc ttgctctttt
     6481 atctcttttt gcttttggaa tgcctctaaa cgttaacggg agactcacat tttccagggc
     6541 agttaaagtt ggtataaggt tataagactg aaaaacaaaa cctatataat cctgcctgaa
     6601 ttttgtcagt ttcctctcat tcagcttctg catttcattg tctttgataa taatacgacc
     6661 ttttgtgggt ttttccagac ccgccataag attcaaaagc gtagatttcc ctgagcctga
     6721 agtgccaaga aaacaacata tttcaccttt tgatatgtta aggctgacat tatccaatgc
     6781 aataactttt tcattgccaa ttctatatat cttgctaaca tcctgcaata ttattatact
     6841 ttccaaatac tttcacaccc cctccacata actattctat taaaatattt gttcaaattt
     6901 gttaaaaatt tattaaaatc aataaatgaa aacttgacct caaagtactt agacgtaaca
     6961 tatattgata ttgtttcaat tttagggaaa tttttgtgaa taataaaata agtatggcat
     7021 cacatcatac tcatttaatg caggttaaag cacatttgaa ttacagacat ggactaagat
     7081 gatttttcta cataatcagt cagcttagcc ataaatttca ctttttgcat tcatatagct
     7141 ttcatatgta ttattatcaa agcaaacgat gaacaccctt gatatactat tattgctttc
     7201 caggaaatta tatatttcgt ttactgcaat tcttgcagct ctgtttaccg ggaacccata
     7261 tgctcccgtg cttattgacg ggaaggctat tgtttttaca cctttttgtg aagcaagctt
     7321 caaggagttt ctgtaacact ttgccaaaag ttcatcttca ccattatttc cgcctgacca
     7381 cacaggtccc acagtatgga taacatatcc agcaggcaaa tcatagcctt gtgtaatttt
     7441 agcctcactg gtttcacatc cacctagatt acggcactct tcaagaagcc ctgggcctgc
     7501 tgcacggtga atagctccat ctacacctcc gcctccaagc aaggtattat ttgcagcatt
     7561 cactatagct tccacatcaa cccgtgttat gtcaccttca ataataatta attttcccat
     7621 aatctctcct aataatttgt atatactcgg ctgaaaacct ccatccttgt tactttggtt
     7681 tttttaattt caagactggt ttacctaaat agccggcaat tttaaacatg tctgtactaa
     7741 tatttctata gtaatatttc tataataata ttatattttt atacttttct actttctact
     7801 tttatccctt gattctttca ataagcttaa tagtataaat gataattggt tggtgtatca
     7861 tataaataat caatgtatgc cttcccaccc agcttattat attatctttc aatgtaaatt
     7921 taaatacgct gtttctatcc ttatataata ctttaccaag tacaatacca tatataaata
     7981 tgccaagcca tggtataagc ggataataat cggcggatac aaatttatta cttgttattc
     8041 cgaatataaa aaaatagtca aaatccagag taatatgttt aataagattt cccaatatca
     8101 ttataattgt tcccaatgct aaaagcaaat ttgtattcag ccttgaaagc aaaggaaaca
     8161 ataatatact tactcccaaa aaatgcaata ctccgaattt aatacccata tccacattat
     8221 ataaatgagt ggcgactgta ataagcatag ccaggccaag tatcttaagc cctcttttaa
     8281 tattatttct gcttaatgta cagcttatac ctgaaagaac aataaacaga gtacctgcag
     8341 ttcttcccgt caaggcatta aatcctttag tataatcaat attataacca aatatatcct
     8401 tcaaattaaa aacaatatgg aaataaatca ttaaaattaa tgccacacct ctaaaaaagt
     8461 ctatttccca tattctcccg ctacgtattt cgtttttttt ctcttcatta ctttgaatag
     8521 caacactttg caatccaata gtcaataaaa cccctccaca ccaagtgtat ttattatgtt
     8581 taaagaaata atgctgattg atacaaaaca caactatgtg ctatactagc attatatatt
     8641 aaagtaccaa atataacaac acaaatgata atagcataaa tgtgatataa atgtggaggt
     8701 tatttatgat aattatagga gaaaaaatta acagtaccat taaatctgta agagatgcta
     8761 tagaaaaaca tgattcactt tttatacagg atcttgctga aaagcagtgc aaggctggtg
     8821 caaactatat tgatgttaat gctggtatgt tttatgataa tgaaccagaa aatcttgaat
     8881 ggcttgttaa aactgtacag gaaggaacag atgcaccgat ggcttttgat tcacccaatc
     8941 cggcagctct ggagaaggct cttaaagtga acaaaaacgg caaaccgatt attaattcca
     9001 ttacggatga aaaagaaaga tatgaatcaa ttcttccact gatattggaa tataatactt
     9061 cagttatagc cctttgtatg gatgatagcg gtatgccaga aactattgaa gatagattcc
     9121 ttgttgcaga aaggctgatt gataaattaa catcaaaggg tgtcaatcct tgtgatattt
     9181 atatagaccc tatggtaaga cctataggta caggttctca ctatggaata acagctattg
     9241 aaactattag gattgttaaa agtaaatacc ctgaagtaca tattgcctgt ggacttagta
     9301 atatatcctt tgggcttcca ggcagaaaag tatttaatca ggcatttttg atagctgcaa
     9361 tgacagcagg tcttgacggg gcaatacttg acccccttga taaaaaactt atgacattcc
     9421 tatatgcaac tgaagcactt cttggtcaag acgaattttg catgaatttt ttaacaaaat
     9481 atagagaagg ggtacttgat atttagttat gaaggtttta ggtatagtga ccgaatacaa
     9541 tccgtttcac aacgggcacc tttaccattt gcttaaatcc agggaggtta catcggcaga
     9601 ttatacagta gctgttatga gcggtaattt tgtccagaga ggtgagcccg ctattgttaa
     9661 taagtgggct cgttcttcta tggcactatc atcaggtgta gatttggtta tagaactgcc
     9721 tgtaatatac gccactgcaa gcgctgagtt tttttcctgg ggtgctgtaa agttgctgga
     9781 tagccttggc ataacagact gtatatgctt cggtagtgaa tcgggagaaa tggagccact
     9841 taacctaatt gcagaaatat tggcaaatga gcctgaagag tataaaaagt tgttgaaaac
     9901 ctgtttgaat atgggattat cctttccagc tgcaagagca aaagccttga aagaatatga
     9961 taattcactg gcagcagacc aaatattgag atattcaaat aatatactcg caattgaata
    10021 ccttaaagcc ttgaaaaaac ttaacagcag gattgagcct tttactataa agcggaaatc
    10081 taatttgtac acagaagaaa atcttacagg tgaattttca agtgcaaaag caataagaaa
    10141 atgtgtatct gaaggggtag ataacagtat tcttttagac aattgcctca ttaaaaatac
    10201 gattccatca gagagcgccg aaattcttaa tacagaattc atgaatggaa gaggcccggt
    10261 attatttaat gattttgaga atattatcct tgcatctttg cgaaagaata gccttgattg
    10321 gataaaatta tacccgtata ttactgaagg tcttgaaaat agaattaaaa atgcagctaa
    10381 aagctctggc tctttacaag aacttattaa taacatatgt tctaaacgtt atacaataac
    10441 tcgtgtccag agaatcatta caagttttct cctgaacata acgcaacagg aatttgaaag
    10501 gttcaatatt tatggagggc cccaatatat ccgggttctt ggctttaacg aaaaaggaac
    10561 acagctgctg tctgaaataa acaagaatgc ctgtctgccc gtaataacta aaactgcaga
    10621 ctttacaaga acttgtaata ttctgctaag aagaatgctt gaaattgaag ccctcgcaac
    10681 cgatatttat gtattggggt attgtaatcc cgattttagg acaggcggcc aggaatatac
    10741 ccagaaccct gtaaggatgc ttaacaaggg tagttctgca gttaataaat aaaaaagtac
    10801 aaaaaacatt aaatattttc aatacctcga tagtatttaa tagtagtata tttaatagta
    10861 gtatatttaa taataaatca attttgttat tataaatagt aaacaccaag taaaatgtaa
    10921 atgagtgatg tgaacaacaa tatggataat gattttgtta ttggcgatat tgaatttaat
    10981 attgatagag caatacaagc tgtcaacgcc tacagtcatt ccacaggtat cagatgtatc
    11041 ataattgatt ccaaaggtaa aaccctttat gagaaatgtg ttgatatgac cggatgtctg
    11101 ttctgtaaaa aagcagaaga tttgttggga gaacatcaga gttgtacaaa tgctcattta
    11161 tacggtagtt accaagctga aagatttggc ggcaaatatg tatttttttg tcctatgggt
    11221 cttgttcact gggtttctcc aattacaaaa aacaacattt taaagggcgc ggtgttggca
    11281 ggacccgtcc agatggttga gcccgaggaa tttcttattg aagatatcct gaggaaaaat
    11341 aatattaaag aggaagatat taatgctata aaagaaagtg tacatgatat acctgtaata
    11401 aaaacggaag ttgtaaacag catgtcggaa atattataca ttgtagctgc ctatatatcg
    11461 aatgtccagc cttcaaaata tcttgaagaa atagaagcgc ttgaacagca atcagacatt
    11521 tcggaatata ttcattatat taagactttc ggtggtgaca gtagtgagct gaacagctac
    11581 ccaatcgaaa aggaaaaaga gcttctaatg cttatttcta tcggcgataa agccggatca
    11641 caaaaaattc tgaatgaaat attcggacac atttttttct ctacaggagg aaattttgaa
    11701 gttataaaag ccagagtatt ggagcttacc gtacttcttt ccagggcagc tctcgaaggt
    11761 ggagctgatg cagagcagat cttcggcctt aattatatat acttgaatga aattcacagt
    11821 tttagcactg ttgaagaatt aacatactgg ctgtcaaaca ttatgatgag attcacagat
    11881 tgtgtgttta cacttgcagg ggttaagcat gttgatgtaa tctaccgggc aattgaatac
    11941 ataaaaagaa gttacatgaa gaaagttaca ctcgaggaag tagcctcata tgtgtacctt
    12001 agtcccgctt attttagtaa aatttttaaa gaagaaatga aaacaaattt taacagctat
    12061 ctcaaccata ttagaattga aatggccaaa aagcttcttt tggatgatac aatatcgtta
    12121 gttgatgtat ccaacgttgt tgggtatgag gaccaaagct atttttccaa agttttcaaa
    12181 aggatggttg gagtaagccc tggaaaatat agggaggcaa gaggtaaaat ataatttttt
    12241 atgttgacta gttaacttat aatcttgatt gaatgcggat aagacggtat tatgaacttg
    12301 aatacaatat aacttacaat aatataacaa aactaatcaa ggaggcatta aacatgagtg
    12361 ttttaaatga aatttcccaa aatctgcagc tgggtaaagc taagaatgta aaagaattgg
    12421 tgcaaagagc tgttgatgaa ggtattgatg ttaaactaat tcttgaagaa gggcttttat
    12481 caggtatgag cattatcggg gagaagttta aaaacaatga ggtatatgtt cctgatgtgc
    12541 ttattgctgc aagggctatg aatgctggta ccgaaatctt aaaaccactt ttggcatcca
    12601 gcggaattaa ggctaaaggt aaagttgtaa ttggtactgt aaagggtgac ctgcatgaca
    12661 taggaaagaa tctggtaaga atgatgatgg aaggtcaggg tcttgaagtt attgatctgg
    12721 gaattgatgt atccgcagag aaatttgtaa aatctgcaat ggaaaacgaa gctggaatta
    12781 tagcttgctc tgctctcctc acaaccacta tgggcgaaat gaaaaatgtt gttgaagaag
    12841 ctaaaaaggt tggaatacga gataacatta ctattatggt aggcggtgct cctgtaaatg
    12901 aaaacttctg taaaagcata ggagctgata tatatactcc tgatgcagca tcagcggctg
    12961 aggaagcagc taaggttttc gcagcaaaat aaattcagta agataggtgt tagaaactgg
    13021 agtggaggct agaagctgcc agacagcagg ttaggtgtta ggtagagacc aatctctaac
    13081 atctagcatc taacctccca ctctgcatct ctaacctccc actatccact atccactatc
    13141 cactatccac tatccactat ccactacccg ctatccacta cccatatccc aataaacatc
    13201 taacatctat atctccatta ttatcggtag aatcataggc tttctttttg tcttttcata
    13261 aagaaaattc cttaaatcat ctttaattat gtttttcact gtagaccaat cggttttttt
    13321 cttgtcatca cattttagaa gagcattcct tgttactaat ctggcctctt ccattaaatc
    13381 ttcagattct cttacgtata caaaacccct ggaaataata tcaggtcctg caataaggtt
    13441 tcctgtttct ctttcagttg ttattacaat aactatgagt ccatcctgag aaaggtgctt
    13501 cctgtcacgc agaacaatac tgcccacatc accaactccc aatccatcaa caaataccct
    13561 gccggatgga acactactgt ttaattttgc ttcttcggct gataattcaa gcactctccc
    13621 tatttccgga ataaatatat tttccattgg cattcccagt tcatgagcca aatttgcatg
    13681 ctgttttaaa tgcctgtatt caccatgaac aggcataaaa aatttgggcc ttataagagt
    13741 atggattaat tttatttctt cctggcatgc atggcctgaa acgtgaatat ctgcgagaga
    13801 ttcataaata acattagctc ctcttttaaa caattcattt attactttga ataccagctt
    13861 ctcatttcca ggtattggtg aagccgatat tataacaagg tctcctggaa ctatctctac
    13921 ctttttatgc tcagatgcag ccattctcga caatgccgac ataggttccc cctgagttcc
    13981 agttgtaatc actactgttt tatcaatagg atatctgtct atatcgtcta catcaataat
    14041 agtaccttca ggtgctgtca gatagccaag ctttgtggca acatccacta cattggtcat
    14101 gcttctaccg catattgcca ccttcttatc gaacttgaca gaagcattta tcacctgctg
    14161 aattctatga acattagaag caaaagtggc tataattatc ctgccttttg ctttcataaa
    14221 aatttcttca aaggtgttgc ctacagtcct ttcagacatt gtataacctt ctctttcaat
    14281 gtttgtgctg tcacacataa gtaaaaggac acccttgctg cccaattccg ccagacgtgc
    14341 aaagtccata ggcttaccct caataggagt atagtctatt ttaaaatcac ctgtatgaac
    14401 aataatgcca agtggtgtaa atattgccaa tgccacagaa tctgcaatac tatgagtaga
    14461 gcggataaac tcaaccttga aacaacccag ttcaatactt tgtccctgct caactctctg
    14521 aagtacaacc tggtcaagca taccatgctc ttccaatttg tattccagca agccaagagt
    14581 aagtcttgtc ccgtataccg gtgcatttat ctgttttaag acatatggaa gagcaccaat
    14641 atggtcttca tgtccatggg ttagtacaat tcccctaatc ttttccttgt ttttaatgag
    14701 atacgaaaca tccggaatga ccaggtcaat acccagcatt tcatcttctg ggaaagtaag
    14761 tccgcaatca actataataa tatcatcctc acactcaaat acagtgatat tttttcctat
    14821 ctcatttact cctcccaagg gtataacttt cattttcttt ttcttttttg ccacaattaa
    14881 acctccaatt aaattaactg attacttcaa ctaaatattt cacggataaa aaatttcgag
    14941 gcgtgacaaa cataatgaca tctgcatcaa attacaatga gcacactttc agctgaaagc
    15001 ctgccaatgt aaatgcagtg tagccattaa gtcattccac atctatagtt ggaaataaat
    15061 ttctcactca ataataataa taaaacattt taatatatta taaagccccg gtatatttaa
    15121 caatatacta acataaatcc agtaagtata ttattaaata tctttattat caccattaat
    15181 aatattaaat catttattat aaactgtcaa tttatcttta ttactttatt gctacttact
    15241 gttatttccc tgtatacttc gaaagtttgg tctgctatgg aatcccagtt gtaaatattt
    15301 ctcactttat caagggcttt ctttttcatt atctcagcct tttcaggatt aaaaagtatt
    15361 tccagaatgc agtctgcaag ggaacttgga ttaccagagt aaaactttaa accatcaacc
    15421 ccatgctcta caatttctcc tagccctcct gcatctgaaa ccacaatagg tacattcgcc
    15481 ataatccctt caagagctac aatcccgaaa ggttcatata ggcttggaaa cactgctata
    15541 tcagcacatt tgtataattt ccccaaaact tcctcactga tgtacccggt aaaatatact
    15601 ttatgctcaa tgttcattgc acagactttt ccatgcagat aatcgagttc aggtccttta
    15661 cctgcaatta caaatttaac atcataataa tggcaaagta ttttcggaat agcatctata
    15721 agtatatgga cccctttctc attaacaatt ctccctacaa aaaaaacgat tttttcattg
    15781 ctcatagcat aattatttct aaattgcatg tctcttgaaa aattatcaaa cttttttaaa
    15841 ttgacaccat ttggtattat tcgtatttta tcttccggca gctgaaaatt attttttact
    15901 tcatagaaca tatatctgct attgacaatc actctccacg attcataagt aagccaccat
    15961 tctacactat ttatatagct ttgtacttca ttatgtattc cactatttct tccggcttct
    16021 gtagcatgta tagtggatac aagaggtgct ttataggaat gctttaatgc tcttgcagta
    16081 aaagctgtca gccaatcatg agcatgtatt atatcaaact ctcctatttc attaaaaagt
    16141 cctattgatc tttctaataa tgaaaaattc atatgaagaa cccaatcaat aaaatttccc
    16201 ggggcaatat cataaatatt tgttctatgg acaaaaactt tctcatcctt ttcatcttct
    16261 gcttcattta catcattttt ttccttccac gtaactatgt gcacttcatt tccctgttct
    16321 cccagttttt gcgccagatt atatacaact cttgctatac cgccaacaat tctgggtgga
    16381 tattcccacg aaagcattaa tattttcatt cttattctcc tttcactaaa tccactcatt
    16441 tatatattat tgttcaactt ttctactttc tttatgtata aataaagcaa tgattttgca
    16501 gtaatactaa tatgagtatt aaatattttc cgaatattat tgagtgattt aattcaaaat
    16561 agtatataat aatgttaatg tcggattaaa tgcagtagaa attcaatggg tttaatgtta
    16621 aattttaaga aaggaggtac gtaatttgcc cgtactggaa gaacatagta aaagtcaata
    16681 tgaactgccg gaaagttatg aagataacaa tatagcaata atggccgtag atgctcatac
    16741 tctatatgct tattgggata tttcaaataa taagatgcag aaccttatca acaatatagg
    16801 cagcatggtt tgggaaaact cggttcctgc acttaaaatt acaaatctaa ctaaaaattc
    16861 aagttttttc gttagaataa acggcttttc tcacgattgg tatataaata ttcatgattc
    16921 cgggtgtata tttgtggctg aattaggcag gaaaattact gatgattatt ttatcagctt
    16981 cgctacatca aatcatatat cactgcctga tacaagtata agctttaata atacagcttg
    17041 ctttgtaaac tgtaaatatg ccatggaaag tgcttttagt ggagatgata atatcccttt
    17101 ggagtacgga attaacgtaa acggtaattt aagaaggata acagccctca tgtttaatga
    17161 agaatttggg aaacttccag atttcgagga aatatatggt agttgtagct ccagcatttc
    17221 ttcaatgttt tattgtgaac aggaaagagt ttcatctttg gaaaatttta attatgaaaa
    17281 aaagaaaatc ctatccttaa gctcttttga gattattaag aaagataaga aataatatta
    17341 gcaaaacaaa gaaagtcaaa agcatagtat aaagttagag atagaaccaa tggcgtgagc
    17401 agagacgctt aataggaaaa tatcagaatg aaaagaaagg agatgccaca acttctgcat
    17461 ttttggtggc agaatttaat tggcaaaggg atacgtagca tttgtattac atgctcacct
    17521 tccatttata catcatcctg aacaggatga ttttctggaa gaaagatggc tttttgaagc
    17581 tatatcagaa acgtatatac ctcttattac tgttttcaac agacttcttg atgaaggctt
    17641 agattatcgc gttactgttt catttactcc tccgcttctt tcaatgctgt caaataacct
    17701 tttgcaaaac aggtatataa gatatctaga gaaacttatt gaattatcag aaaaagaaat
    17761 taaaagaact gaaggtcaga ttgattttaa caggctggcc aggatgtaca atgataaata
    17821 taaaaatgac ttgtacatat tcagagataa gtacggcaag gatcttgtaa atgcctttaa
    17881 aaacctttat gactcaggga aatttgagtt cattacctcg gcagcaacac acggttatct
    17941 tcctctttta gctataaacc ctgaaagcat aaatgcccaa attgcattag gagttaaatc
    18001 ttttcaaaga tttttcggtt ttaaacccaa aggcatgtgg cttccggaat gtggatatgt
    18061 tcatgctatt gaaccagtac tcaaaaaata tggagtagaa tatttttttt cggaagcaca
    18121 tggtatactt tttgctgatc caagacccgt atttggaacc ttctcaccaa ttataacccc
    18181 gaagggtatg gtagtctttg gtagagatac ggattcctca aagcaggtat ggagttcaaa
    18241 agaaggatac cccggtgact ttgattatag agagttttac agggatatcg gttatgatct
    18301 ggactatgaa tacataaaag aatatatatc tgcagatggt cagcgcatag ataccggtat
    18361 aaaatattac agaataacag gcaacactga aaataagcag ccatataacc ccggttgggc
    18421 gcaaaacaaa gcagatttac atgcctttga ttttatgtat aacagacaaa aacaaattga
    18481 atacctttct gatataatgg ataaacctcc aataatagtg tgcccttatg atgctgagct
    18541 tttcggtcat tggtggtatg aagggccata ttggatatat tctctgttca aaaaaatgga
    18601 tgttcccggt aatcctatta agcttacaac acccggggaa tacataaatg gaaaccccat
    18661 tatgcaggta tcttcacctt gcccatcgtc ctggggttat aacggttata atgaagtctg
    18721 gcttaataaa actaatgatt ggatttatcc tcacctgcat aaagcagctg aaaggatgac
    18781 agaactggca aacgaaaaca aaaatgctac gggtttgaaa caagacgcct taaatcaagc
    18841 agcaagagag cttctcctag cacaaagcag tgactgggct ttcataatga aaaccggcac
    18901 tatggtccgc tatgcagaaa aacgggtaaa agatcacata ggcaggtttt taaagcttta
    18961 tcatgatatt aaagaaggcg taatagacga aaactggctt agagatattg aatataaaga
    19021 tgacattttc cctaacatgg attatagttt atttactagt ggaggatgaa tatggctgaa
    19081 ttcgtgcatg tatttcacaa gaaaaccgga tataactgcc ccatatgcaa ttcggaaatg
    19141 catatggttt actggcgtga taaatcaaga agtttcatgt gcataaaatg cttcttcggc
    19201 tttaattata aataggaaaa taactggcaa ctattgcttt cttctctcgt taatactatt
    19261 tattgtaaat aatgttatat ttcgtatttt taatgtttac aagtagagca atatgtagta
    19321 ttataattat gtaaatgagg tattttgtgt aaattgtaaa gggaaaagtc agattggagg
    19381 agctatagta atggagaatg aaaaattatt tgaatttttg acaataatgc attccgaaat
    19441 gaaggaaggc tttagtaaga ttgatgagcg ttttagtgag attgatgagc gttttagtga
    19501 gattgatgag cgttttaatg agattgatga gcgtttcagt gaagttaata acagactgac
    19561 caagattgaa aatactgtaa taaaaattga gaacgagcat ggcaaaaagc ttcaggttct
    19621 attcgatgga tacaggcaac attcagacca gctggagcgc attgaaaaag aagtcaccaa
    19681 acatgatgaa tttatattaa agagagtaaa ataaaattaa atatgttaca ggacacattt
    19741 tattatttat aatatcaaac gatgaaaaaa taactggtga aaaaatctga aaaaagttga
    19801 attagctctt gcaaaaagta gatgatgatg gtataatatt tttcgttgag ccgatgcggg
    19861 attgtgtaat ggtagcacga atgactctgg atcattttgt gagggttcaa atccttctcc
    19921 cgcagccata caaaaatcca caatggtaat aatttttata tactcattta ctcattgtgg
    19981 atttttgtat ttttaaatag gtctgaataa aatttgatct attttcgtct gaattccaaa
    20041 ggtgatattc cggatttctt cttaaatact cggttaaaat agcttacgga actaaatcca
    20101 caggaaaaag caatttcagt aatattcata tttgtcgtaa ggagcagttc ctgggcatat
    20161 aaaactctga catcatttaa atattgtgta aatgtatatt ttgtagcttt cttgaagcat
    20221 ttgcagaagt gttggttgca catacccgta agggatgata catcatttat tgatatggaa
    20281 ttgttataat ttttaaagat ataatctata gctggagata tcttttccag taaaagcttt
    20341 tgcttcatat attcttcagc cctgctatct acaaactggg tatttctgta tataataccg
    20401 agaagcttat acatatgaga tgccaaataa tatccatatg cactattttt atttataaat
    20461 tcttctataa tatttgccat acaccgttgg atttcaatat ttacttcatc gctcccatta
    20521 aaagggaaat aattgcttat tatcttatga ttccagtgct ctctgtaata taaactgata
    20581 aaagaaggtg gtatctgcat aataagtatt tttatgtata tgttggggtt caggtacaat
    20641 gaatggaggt ccaaaccatt tatgtatgcc atgtcccctt ttttcattct gtaagtattg
    20701 ccattaatcc tctgatcaac agccccgcaa atcacatata caatctctgt aaattcatgc
    20761 cacaggtcat tataatcatt atgctgctgt ttttgactat ctatatatga ccttatatca
    20821 cttctttctg aaatccatat attaaaaggc aatccctcat ctcttaaatt tacatactcc
    20881 ttaaatgctc tcattattct ccacttctat atttcattat ctcttctaaa atattaatgc
    20941 cggctatttt cttaagttat attatagtat atcggtagac aaatacaagt gtatgatgaa
    21001 gtgatgaagt gcgggtgcat ttaaaatttt tgaagaaata tattccagac gctatcgtta
    21061 cggtacttag agcctttaaa ttcacctaca ggcagaatca aactcacaaa ataatctgaa
    21121 taaaaaatag taaaaaatag ttattattct gttcatttgc aagtttaaat tttttttatt
    21181 catacttgtg tatttacgtt tattaaaaaa actatgtcta taatttcaca atgcattgat
    21241 tttagctgat tttatgatat aatattttat aagaaatctc tgtagttttt acttttttgt
    21301 ggactatgtt gattttttaa caagttgaac ttgcataaac attatttgga gggggttaaa
    21361 atatgaaaac ttctgacctg ttgatttcca gtgtaaacat tactgaagat gaataccact
    21421 ataaaggtca atttgttcta agttcagaac ataaatcttt aattgtagat ttagccgtac
    21481 tatatgatga taacgaaatt aacaatatta aggaagaatt taatttgcag gaatccaaca
    21541 gtgaaatacg agaatctcta ttaaacagac ttgtcataaa agcaggtata tcctcaaaaa
    21601 ttgtccaggg tgaggactat tttgagctaa aagcaaagag aacataaaat tggggcgcat
    21661 tttttcatat tttacatcga gaaaaaatgc gccctgattt ttacttactc tttattccgg
    21721 tttatttgct ttcatttgcc attaattacc gcttgggcgg ctgcaagcct tgcaataggt
    21781 actctaaagg gtgaacatga aac
//
LOCUS       DAOSDD010000051        21761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1050_length_21761_cov_4.915461, whole genome shotgun sequence.
ACCESSION   DAOSDD010000051 DAOSDD010000000
VERSION     DAOSDD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21761
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_21761_cov_4.915461"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK733_06550"
     CDS             <1..243
                     /locus_tag="PK733_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HOJ10237.1"
                     /translation="SQKGYDGVIQIYPLTCMPEIVAESILPAVERDYDIPILTLIIDE
                     MTGEAGYLTRVEAFIDLLYRRREKTRVEGKYILSGD"
     gene            209..1381
                     /locus_tag="PK733_06555"
     CDS             209..1381
                     /locus_tag="PK733_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ10238.1"
                     /translation="MLKGNTYYLGIDVGSVSTNLVIIDNNDNIQEKIYLKTNGQPIKA
                     ICEGIGYIRRRLYEGPQDAGIKAQHEDGKDPHINEKAKYSAENNRGQAISAIMNFKEE
                     LSPELLENTGENQNEKVPPEIKGVGVTGSGRQLAGVIAGADVVKNEITTHAVAAQKIV
                     PDVKTILEIGGQDSKIIILKNGVVYDFAMNTVCAAGTGSFLDRQAARLGIPINEFGCY
                     ALRSGSPVRIAGRCAVFAESDMIHKQQAGHSLEDIICGLCEGLVRNYLNNLAKGKKIE
                     DPIMFQGGVAANVGIVAAFEKIFGKKVIIPEYFDVMGAYGAALIAKEKMTGVEADRES
                     GKEAKTKFRGFEAAENHYNTKSIECNGCSNMCEIVEILSDDRVIARWGDRCGKWSM"
     gene            complement(1402..2778)
                     /locus_tag="PK733_06560"
     CDS             complement(1402..2778)
                     /locus_tag="PK733_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOJ10239.1"
                     /translation="MLAGKLKSEAGKFSSKTGKFSMVSIYRRLMHRYAHRIKVFFVML
                     ILCLISLHILAAPFACAAHTIQVALDDGKPESPIIKAPSAILVEAERGQILYKKQADK
                     KLHISTANKIMTAILAIEKNDLGAKVTISKESSSSEGSLLFLAIGEKYTVEDLLNAII
                     ITNANDAANALAEYAGGDINMFVEMMNKKAQALNMQDTNFTNPTGLYDSAQYSTAKDI
                     AVLIRYALSLPDFNRIISYRAVPWVDKQGVDLLTNTNKLFWSYDGVDGGKTGFNNKEQ
                     QTAITAATRNNIRLISIVLDAPENSVFSDSSNLLDYGFNNFKKGILVSKSQDLKSISV
                     GSKEIKLISANDVYYIYPVGENYVRSISFNIVKDFKPPITRNSLAGIAHYTLKDGTVI
                     DVNLYPETDILPPEKFYTKLINKLKESKDLYILVIMLIIMEILIMFYKIGIKISRISN
                     RRKNGPGT"
     gene            complement(2868..5552)
                     /locus_tag="PK733_06565"
     CDS             complement(2868..5552)
                     /locus_tag="PK733_06565"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HOJ10240.1"
                     /translation="MAGNINEKLKTQNAKMEDILFHTFSVEKTIEKLNADKNGLSEAE
                     VEKRKSKYGGNVLDEGKRKSLTAMFLEQFKSVMIIILLVAAIISGFLKEITDTIIILS
                     VVLINAVLGVVQENKAEKSLEALKKMSTPYSKVKRGGEVTLLKTEEIVPGDIVLLEAG
                     DFVPADMRLIETVNLKIEEAALTGESEPVEKISREINDRDIVIGDRKNMAFSGSGVTY
                     GRGVGIVTATGMNTEVGKIAGYLTQNEAEDTPLQKKLSEMGKYISVGVVAIAVIIFIA
                     GILKGREVFEMFLTSVSLAVAAIPEGLPAIVTIVLAVGVQKMAKRNAIIRKLPAVETL
                     GSTDVICSDKTGTLTQNKMTVMEVYFSGISKNALDVSVKDDGFNTFAQIMTLCNDSKV
                     SIKDNNKAEMVGDPTETALVAFTFQKGLTKEKLEKEIPRVNEIPFDSERKLMTTINKM
                     NNGSLRVMTKGAPDMLLSRCSKIYINGEIVPATQSHLEEIIQVNSQMAGKALRVLAMA
                     FKDIEEIPENFTSESIESDLIFAGLTGMIDPPREEAKKAVEICKQAGIKPVMITGDHK
                     DTAVAIAKELGILEDRDLAITGSDLDKIIDDNFSDQVEKYSVYARVSPQHKVKIVKAW
                     KEKGKTVAMTGDGVNDAPALKTADIGVGMGITGTDVSKSVSDMVLSDDNFATIVTAVE
                     EGRRIYGNIRKAIQFLLSANIGEVLTLFAATMLNWTILFPIHILWINLVTDTFPALAI
                     GAEKAEKDIMTYKPGKTGDNVFSGGLGFSIVYQGILEAVITLLVYYTGQKFYTTSVAT
                     TMSFAVLGIIQLMHTFNVRAGEKSAFSIKLFSNRYLLLSVALSALLQLIVILIPSLNS
                     IFRVTPLSGKQWLIVVIASFSIIPAVELVKLIHKLGKK"
     gene            complement(5918..6427)
                     /locus_tag="PK733_06570"
     CDS             complement(5918..6427)
                     /locus_tag="PK733_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOJ10241.1"
                     /translation="MPGEKHFKNAFLGFNKDDVNSYIENLLQDFESKLKELEMEIANL
                     KSENRDLKTKYEELANREEQINIEREKIAEVLIKAQEKAQLMIEDARIKALDEKNKIQ
                     EMTEHEKEKLVDIKEEIRMLKKSISSTLKKYETQLGSIINETAGSDYAEDHSIASAEA
                     AAISDVQAG"
     gene            complement(6603..6932)
                     /gene="trxA"
                     /locus_tag="PK733_06575"
     CDS             complement(6603..6932)
                     /gene="trxA"
                     /locus_tag="PK733_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ10242.1"
                     /translation="MASEKVLELTGSNFDTEVIDSGKLVLVDFWAEWCGPCRAVAPVI
                     EKLADDYDGKVVIGKLNVDNESELAAKFRIMSIPTIALFKDGKMVERIVGAKPAQEFK
                     TIIDKYL"
     gene            complement(7102..7383)
                     /locus_tag="PK733_06580"
     CDS             complement(7102..7383)
                     /locus_tag="PK733_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOJ10243.1"
                     /translation="MDLQKLNIMKRLNRIEGQVKGIQRMVNEDKYCMDVLTQVAAVRA
                     AINKVGVIILENYSKTCLKSAFDSEDSDKRAKAFDELVNTINKFLKFIE"
     gene            7889..10456
                     /gene="glgP"
                     /locus_tag="PK733_06585"
     CDS             7889..10456
                     /gene="glgP"
                     /locus_tag="PK733_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ10244.1"
                     /translation="MYITGKITVNPVLPEKLARLKDIAYNLWWSWNVEALDLFIDIDA
                     ELWESVRHNPVRFLHEVSCSKLDAAQNNENYMDKYNYIIEKFDSYINDKNTWFNKNYP
                     ELNNHGVAYFSAEYGLNEILPVYSGGLGVLSGDHCKSASDLGIPFTAIGLFYKQGYFS
                     QRINREGWQETAFTDLNITRLPLNPVLNSTGQHVMISVELPGRVVYAKIWEVKVGRIS
                     LYLMDSNVEQNNETDRWLTSRLYGGDQETRIQQEILLGIGGIRVLEALEISPSAYHMN
                     EGHSAFLGLEIMRKLIQEKGLSFKVAKEVAASCIVFTTHTPVPAGNDVFPLHMMDRYF
                     SNFWNKLGISRHELLDLGLKVNDSQNFNMTVLALTLAGRKNGVSELHGAVTRNIFSNL
                     WPGIPEDEIPVSHVTNGIHTLTWLAGELNDLYGKYLKVGWQENISRKETWQDIDKIPD
                     ELLWSTHVKLKKELIEFLRVKIKNRRISNGESSDSIREAGNLLDPNILTIGFARRFAT
                     YKRANLVFRDIERIKRIINNPDMPVQIIFAGKAHPADRPAHEVIKNINDIAGQEGFKG
                     KIILIEDYNMEVARKLVHGVDLWLNNPRRPLEASGTSGQKACINGVINFSVLDGWWCE
                     GFNGSNGWIIGDDTFYDNEYYQDNADSVSIYGTLEKVIAPLFYSKDNNGVPAGWVRVM
                     KESIKSLIYRFSTDRMLEDYTNTMYAPSMERMEKFISGSFSYSKKLAEWKSLIEKDWP
                     QITIRSDKTKNQLKEYNVSSCEKLTLAVTVDLGDIDPSYVKVESYYGYIKNSNHIEDP
                     KIIEMKPVEKNGPSSWLYTAEIRLSDGGEYAYTFRVIPYHPDLISKFEMGLIRWVVS"
     misc_binding    10600..10890
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10948..11451
                     /locus_tag="PK733_06590"
     CDS             10948..11451
                     /locus_tag="PK733_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ10245.1"
                     /translation="MENKNLEFTEEILEILENNCKYSEEQIAVMLNRDVEYVREAIKK
                     LEDENIIAGYRTVVDWEKMPKETVTALIEVKVTPQRGEGFDKVAERIFRYPEVKACYL
                     MSGGFDLTVIIEGKTLKEVAFFVAEKLAPLDSVLSTSTHFVLKKYKDNGTIFEKDEKD
                     NREVVIL"
     gene            11448..12617
                     /locus_tag="PK733_06595"
     CDS             11448..12617
                     /locus_tag="PK733_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ10246.1"
                     /translation="MNITEMISPLVRNIPPSGIRKYFDMINEMEDVISLGIGEPDFVT
                     PWNIREAGIYSLEKGDTHYSPNSGYAELRNEISKYLNRRFEVQYNPKNEMIVTVGGSE
                     GIDIALRALVSAGDEVIIPEPCFVAYKPCTIFAGATPVTINLREEDQFKLTPELLKKA
                     ITPKTKVLILPFPNNPTGAIMTREDLEGIVDVLKKKDIIVISDEIYAELNYEGKHVSI
                     ASFPEMRDKTLIINGFSKAFAMTGWRLGYACGHPDLIAAMLKIHQYAIMSSPTTAQKA
                     AVEALKNGDKDVYTMVREYNRRRRVMVAGFRKAGYNCFEPLGAFYVFPCIKSSGMSSE
                     LFCENLLIEEKVLVVPGTAFGRCGEGFVRATYANSMENIIEALKRIQNFAGKHIK"
     gene            12719..13411
                     /locus_tag="PK733_06600"
     CDS             12719..13411
                     /locus_tag="PK733_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10247.1"
                     /translation="MGFALNTKKLREQNKLSQKEIADYLGVTRQAVASYESGKREPDY
                     GTLIKLSDFYGVSVDSLLGRSQCKDINVLTIAKNIVLIKGDFNYEEFSRDIFRKTGVC
                     ILENMLKLYAEGEKIPAIGTIKVLAKYASVHESFFYKFNTLEDYANEKALYDAKTLCN
                     EKTSYDIFSCNLDFMDPQLAGWVQDEKNMHYIMLAKEIQDTNLPLESVKSILSVLISV
                     QTVTENSKKQNV"
     gene            13484..14594
                     /gene="prfB"
                     /locus_tag="PK733_06605"
     CDS             join(13484..13558,13560..14594)
                     /gene="prfB"
                     /locus_tag="PK733_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149678035.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOJ10248.1"
                     /translation="MLELEQYKLEIRDLGEEITEMRDSLDLPVLKNELDELEQKSAEP
                     EFWNDMEKSQKILQRIKTLRDKLETFGHLESTLEDLKTLNELGIEEDDESVIDEVKEG
                     IEELKGKIAKLRTETLLTGPYDKNNAILSMHAGAGGTEAQDWVQMLLRMYTRWGESKG
                     YTVKILDYLDGDEAGVKSVTIHVIGENAYGYLKSEKGVHRLVRISPFDASGRRHTSFA
                     SIDVMPELDDDIEIEINPDDLRVDTYRSSGAGGQHVNKTESAIRITHIPTGIVVSCQN
                     ERSQFQNRDTAMKMLRAKLFELKEQEQKQKIEGIKGVQMEIAWGSQIRSYVFCPYTLV
                     KDHRTNYEEGNVNAVMDGEIDEFINAYLSEVGSEK"
     gene            14637..15563
                     /locus_tag="PK733_06610"
     CDS             14637..15563
                     /locus_tag="PK733_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621580.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOJ10249.1"
                     /translation="MLYNRFSEYLKKRYNAKVYKLPVNIPVSCPNRDGRVSSNGCIFC
                     GEEGAGFENLPAALGVKEQIRINAEHIAAKYKPEKYIIYFQNYSNTYLPIEPFKKYII
                     EACVENAAAIYISTRPDCINEEQLDFLRNIKNTQKIDILIELGLQTVNYKTLKFLNRG
                     HTLAEFIDAVLSINKYGLESCAHYIIDLPMDSMEDVMEGAKILSALKVNQVKCHSLYI
                     LKDTVLGEMYVKQQIMPVTLEEYIERTITFLEYLNPEIVIQRLLGRAPEDRTLFCNWG
                     MSWWKIRDLVEAKMIKEERYQGKYFNYLGGII"
     gene            15689..16945
                     /gene="guaA"
                     /locus_tag="PK733_06615"
     CDS             15689..16945
                     /gene="guaA"
                     /locus_tag="PK733_06615"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOJ10250.1"
                     /translation="MKNEMLLIVSFNGRISQTIARMVRELGVYTEVIPYNASIDKIKA
                     AGPRGVIITTSQNQGLDERLDFIKKEISEIDIPFLEIAAPDNNFLRSFIHDTCKCSGG
                     WDMKSFVAQSIRNIKEKVGDKKVLCALSGGVDSSVCAVLVNEAIGKQLTCIFVDHGLL
                     RKYEVEQVEKLFREKYDINLVKIDATDRFLGKLDGVTDPEKKRKIIGEEFIRVFEEEA
                     KKIGAIDYLVQGTIYPDIIESGTENTGMVKSHHNVGGLPENIEFNEILEPLKYLFKDE
                     VRKAGLELGIPESIVWRQPFPGPGLGVRVIGEITKEKLDILRDADYIFREEIAASGLD
                     KEINQYFAVITDMRSVGVKEGKRTYDYTVALRAVKTTDFMTAEWVRIPYEILGKVSER
                     IVNEVGHINRVVYDITGKPPATIEWE"
     gene            complement(17114..17281)
                     /locus_tag="PK733_06620"
     CDS             complement(17114..17281)
                     /locus_tag="PK733_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10251.1"
                     /translation="MNIKYIGNDTKKSENGKIHIRKGNYTGCGARIDDNPGDWVSTTQ
                     AVTCDKNGCKN"
     gene            complement(17360..17872)
                     /locus_tag="PK733_06625"
     CDS             complement(17360..17872)
                     /locus_tag="PK733_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10252.1"
                     /translation="MSIGERIRFIRNLRGMTQKYLGIAAGFDEKTADVRMAQYEAGTR
                     TPKGNLINSLAAALEISPEALTVPNIDSDIGVMHTLFALEDLYGLKIKIIDGEICLAL
                     DRSKGTPYLSMYEMFSTWQKEYDKFKRGEITKDEYDDWRYNYPKVEAKRAKQRLDALR
                     SEKKNTDMDE"
     gene            18214..18426
                     /locus_tag="PK733_06630"
     CDS             18214..18426
                     /locus_tag="PK733_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10253.1"
                     /translation="MSNTFMKVDEVAAELGVSKSYAYKIVQKLNEEMKKMGYLTISGR
                     VNKQYFMKKTCYNDTKKKECDEHASL"
     gene            18413..19495
                     /locus_tag="PK733_06635"
     CDS             18413..19495
                     /locus_tag="PK733_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ10254.1"
                     /translation="MPVYKDKNGTWRAVYRYKDWKGEHKQTQRRGFILKKDAQAWERE
                     QLQKIQGELDMTFESFVETYTADVKNRMKENTWETKEHIIRTKLTPYFGKRKLCDIQP
                     KEVIAWQNEMINYKDENGKLYSPVYLKTLHNQLSCIFNHAVKYYGLSQNPAAKVGNMG
                     RARNREMLFWTKDEYLKFADAMMDKPMSFYAFELLYWCGIRQGELLALTPRDIDFEKK
                     TVSITKSYQRIKGRDVITTPKTPKSNRVIKMPQFLCDEIQEYISMLYGTEPTDRLFPV
                     TKYYLHHEMERGCKETGVKKIRIHDLRHSHISLLIDMGFSAIAIADRVGHESIEITYN
                     YAHLFPSRQTEMAERLDFERTEGEEG"
     gene            19499..19819
                     /locus_tag="PK733_06640"
     CDS             19499..19819
                     /locus_tag="PK733_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004010381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10255.1"
                     /translation="MSLKNLDRKNRWRNKTVAFRVSPEEDRQIETAVRLSGLTKQDYI
                     IRRLQDREIVVVGNPRVYKALRNQLAAVLEELKRISIGEQIDDELTDTIRLIAVTLGG
                     LKGE"
     gene            19822..20964
                     /locus_tag="PK733_06645"
     CDS             19822..20964
                     /locus_tag="PK733_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10256.1"
                     /translation="MAAKEKATAIASSAPTDEKQSLNLCNNIIMSENSKKINQKNENT
                     RIQFLFRQMNNPDYFNTVSLTELYDTVYQPKTQVIDNLLYAGVYLFAGAPKVGKSFFM
                     AQIGYHVSKGIPLWNYTVHKGVSLYLALEDDYARLQKRLSKMFGMDGTDDFHFVTNAK
                     NLSQGLESQLDTFIQGHPDTRLIIIDTLQKIREAGGEKFSYASDYEIVTKLKQFNAEH
                     NVCILLVHHTRKQNADDCFDTISGTNGLLGAADGAFLMQKEKRTDNIAVLDLVGRDQQ
                     DQRLHLQFDRERCVWQLIKAETELWKEPPDPILSLISGLVNKEAPEWIGSATELIERL
                     KLEMQPNVLTRRLNVGADWLWNEYGISYENTRSHTGRQVKFTLNGK"
     gene            21312..>21761
                     /locus_tag="PK733_06650"
     CDS             21312..>21761
                     /locus_tag="PK733_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HOJ10257.1"
                     /translation="MVGKGSINHNSRKFHAENTDPERSNLNHTYCNIPIKQIYHELFD
                     EAVKRYNEKQTRKDRCIDDYYEKIRSGKQEKPFHEIVVQIGNKDDMNAKTEEGHLAVR
                     ILNEYMQEFQNRNQNLKVFSAHLHMDEATPHLHIDFVPFTTGSKRGLD"
BASE COUNT         7059 a         3671 c         4444 g         6587 t
ORIGIN      
        1 tcacaaaagg gttatgacgg agttattcaa atatatccct tgacatgcat gcctgaaata
       61 gtagcggaaa gcatacttcc tgctgttgaa agggattatg atattccaat attgacattg
      121 attattgatg aaatgactgg agaagcaggg tatttaacga gagttgaagc ttttatcgat
      181 ttattgtata ggagaaggga gaaaacacgt gttgaaggga aatacatatt atctggggat
      241 tgatgtagga tctgtaagca ccaatcttgt aataattgat aataatgata acattcaaga
      301 gaagatttat ttgaaaacca atggccagcc aataaaggct atttgcgaag gtattgggta
      361 tattagaaga cgtttatatg aaggaccgca agatgcaggt ataaaggcgc aacatgaaga
      421 tgggaaagat ccgcatataa atgagaaagc gaaatattct gcagaaaaca ataggggaca
      481 ggcaatttct gcaatcatga attttaaaga agaactaagt cccgaacttt tggaaaatac
      541 aggggaaaat cagaatgaaa aagtccctcc tgaaattaaa ggagtagggg taacaggaag
      601 cggaagacag cttgcaggag ttattgcagg agctgatgta gttaaaaatg aaataacaac
      661 tcatgctgtt gcagctcaaa aaattgtacc tgacgttaaa actatattgg aaattggtgg
      721 tcaggactct aaaataatta tattgaaaaa cggtgttgta tatgattttg ccatgaatac
      781 tgtgtgcgct gcaggtacag gctctttcct tgataggcag gcagcaaggc tgggaattcc
      841 aataaatgaa tttgggtgct atgcacttcg ttccggctca cctgttagaa tagcaggacg
      901 atgtgcggta tttgccgagt cggatatgat acacaagcag caggcaggtc atagtctgga
      961 agatattatt tgcggattat gcgagggcct tgtacgaaat tacctgaaca atttggcaaa
     1021 gggaaagaaa atagaagatc ctattatgtt tcagggagga gttgcagcaa atgtaggcat
     1081 tgtagctgca tttgaaaaga tttttgggaa gaaggttatt atacctgaat actttgatgt
     1141 aatgggagct tatggtgcag cattaatagc taaagagaag atgacaggtg tagaagccga
     1201 tagagaatct ggtaaagaag ctaaaacaaa gttcagagga tttgaggcag ctgaaaatca
     1261 ctataataca aaaagtatag aatgcaacgg atgttccaac atgtgtgaga tcgttgaaat
     1321 cctttcggat gatagagtaa ttgctcgttg gggagatcgt tgcgggaaat ggtctatgtg
     1381 aattaattat gaatttatag attatgttcc cggcccgttt tttctacgat tggaaatccg
     1441 ggagatttta attcctattt tataaaacat tatcaatatc tccataatta tcaacataat
     1501 taccaaaata tagagatcct tgctttcctt aagtttattg atcaattttg tataaaactt
     1561 ttcaggaggc aatatgtctg tttcaggata aaggttaaca tcaataacag taccatcctt
     1621 taaagtataa tgagctatac ctgcaaggct gttccttgtt attgggggtt tgaaatcctt
     1681 tactatatta aagcttatac tacgcacata gttttcacca acaggataga tgtaatatac
     1741 atcattggcg ctaataagct ttatttcctt tgaaccaaca gaaatgcttt taagatcctg
     1801 actttttgat accagaattc cttttttaaa gttgttgaag ccataatcca gaaggtttga
     1861 agaatcagaa aaaacgctgt tttccggtgc atcaaggact atggaaataa gtcttatgtt
     1921 attgcgcgtt gccgcagtta ttgctgtttg ttgttcctta ttgttaaatc ctgttttacc
     1981 tccatcaact ccatcataac tccagaataa tttatttgtg ttggttaaca aatcaactcc
     2041 ctgtttatca acccatggta ctgcacggta tgagataatt ctgttaaagt ccggaaggct
     2101 taatgcatat cttattaaaa cagcaatgtc ttttgcagta gaatattgag cactatcata
     2161 taatccggta ggattcgtaa aatttgtgtc ttgcatattt aaagcctgtg cttttttatt
     2221 catcatttcc acaaacatat taatatcccc gccggcgtat tctgccagag cattagctgc
     2281 atcattggcg ttggtaatta ttatggcatt caataaatct tctacagtat atttctctcc
     2341 aattgcaagg aataggaggg agccttctga ggatgaagat tctttactga tagtaacttt
     2401 ggcccccaag tcattctttt caatagcaag aatagctgtc attattttat ttgctgttga
     2461 tatatgaagc tttttatctg cctgtttttt gtagagaatc tgccctcttt ctgcttctac
     2521 aagtatggca gaaggagcct ttattatagg actttcaggt ttaccatcat caagagctac
     2581 ctgaatagtg tgagcagcgc aggcaaaagg agcagccagt atatgcaagg atattaagca
     2641 taatattagc ataacgaaaa atactttgat tctatgtgca tatctatgca tcaatcttct
     2701 gtatatggat accatagaaa atttgcctgt tttactagaa aatttgcctg cttcactttt
     2761 tagcttaccc gcaagcattc aaatcacacc cctaactttt tactcctaat tatattatat
     2821 attcatatcg ccattagtca attaattgtt atattaccgg gcgaatatca ttttttccca
     2881 agcttatgga ttagctttac caattctact gcagggatta ttgaaaatga agctataaca
     2941 actataagcc attgttttcc actaagtggg gtaactctga aaatgctgtt aaggctggga
     3001 atcagtatta ctattaattg tagtaaagca gataaagcta cggataacag cagatacctg
     3061 tttgaaaaca gttttatgct gaaggctgat ttttcaccag cgcgtacatt aaacgtatgc
     3121 ataagttgaa tgatgccaag gacagcaaag gacattgttg tcgcaacact tgtggtatag
     3181 aacttctggc ctgtataata tactaaaagt gtaatgacag cttcaagaat accctggtaa
     3241 actatgctaa aacctaatcc acctgaaaat acattatcac ctgtttttcc aggtttgtat
     3301 gtcataatat ctttttcagc tttttcagct ccaatagcca gtgcaggaaa agtatcagtt
     3361 acaagattta tccataatat gtgtatagga aacaatatag tccagtttag catggtggcc
     3421 gcaaaaagcg taaggacctc tccaatattt gcagaaagga ggaattgtat agctttgcgt
     3481 atattgccgt atatcctcct gccttcctca acagctgtaa caattgtggc aaaattatca
     3541 tctgataaaa ccatatcaga gacgctttta gaaacatcag tacctgtaat tcccatacct
     3601 acacctatat ctgcagtttt tagagcggga gcgtcattta cgccatctcc ggtcatagca
     3661 acagttttac ctttttcctt ccaggcctta actattttta ccttgtgctg tggcgaaact
     3721 ctggcgtaaa cagagtattt ttcaacctga tcagaaaaat tgtcatcgat tattttatcc
     3781 agatcacttc cagtaatggc aaggtctcta tcttccaata ttccaagttc tttcgcaatt
     3841 gcaacagcag tatccttatg gtcaccggtt atcataaccg gttttatacc agcttgttta
     3901 caaatttcaa ctgctttttt tgcttcttcc ctgggtggat ctatcatgcc tgtaagacct
     3961 gcaaatatca agtcagattc aattgattct gatgtaaagt tctcaggaat ttcttcaata
     4021 tctttaaatg ccatagccag aactctcaaa gccttacctg ccatttgact gtttacctga
     4081 atgatttctt ccagatgaga ctgagtagca ggtactatct ccccatttat atatattttg
     4141 ctgcagcggc tcagcagcat atcaggggct cctttggtca ttacccttaa gctgccgtta
     4201 ttcattttgt ttatagttgt cataagtttt ctttcagagt cgaaaggtat ttcatttact
     4261 ctgggtattt ccttttcaag tttttcttta gttaatcctt tttgaaatgt gaatgccaca
     4321 agagctgttt ctgtagggtc tccaaccatt tctgctttat tgttatcctt tatactgact
     4381 tttgaatcat tgcaaagagt catgatctgg gcaaaagtat taaaaccatc atcttttaca
     4441 gaaacgtcca gggcattttt tgatatgccg ctaaagtata cctccattac tgtcatttta
     4501 ttctgagtaa gagttccggt tttatcagaa catataacat ctgtacttcc aagagtctct
     4561 actgcaggaa gtttacggat tatagcgttt cttttagcca ttttttgtac gccaactgca
     4621 agtactattg taactatagc aggaagtcct tcaggaatag ctgcaactgc cagactaacg
     4681 gatgtaagaa acatttcaaa aacttctctg ccttttagaa tacctgctat gaatataatg
     4741 actgctattg ctactactcc tacactaatg tacttaccca tttcagagag tttcttttgg
     4801 aggggagtat cttcggcttc attttgtgtt agatatccgg ctattttacc aacttctgta
     4861 ttcatacctg tagctgtaac aatccccacg cctcttccat atgtaacgcc gcttccagag
     4921 aaagccatat tctttctatc gcctatgact atatctctat catttatttc ccgggatatt
     4981 ttttcaaccg gttctgattc tcctgtcagt gctgcctctt caattttaag gttaactgtt
     5041 tcaataagtc tcatatctgc aggaacaaag tctccggctt caagaagaac aatatctcca
     5101 ggtacaattt cttcagtttt taatagagta acttctccac ctctttttac tttcgaatag
     5161 ggtgttgaca ttttttttaa cgcttcgagg gatttctcag ctttattttc ctgaacaact
     5221 cccaggactg catttattaa cactactgaa agaattatta tcgtatctgt gatctctttc
     5281 aaaaaccctg aaataatagc agctactaaa agaattatta tcattacact tttaaattgc
     5341 tccaaaaaca tagctgtcag tgactttctt ttaccttcat caaggacatt gcctccatat
     5401 ttggattttc ttttttcaac ctctgcctct gacaaaccat ttttatcagc atttaatttt
     5461 tcaatggttt tttctacgga aaaagtatgg aagagtatat cttccatttt tgcgttttga
     5521 gttttaagct tttcattaat attgcctgcc ataaaaatat gcctccctga atatggttat
     5581 taatattata cactaactat tatgtatcta aacattatgc caagtaggac cagcctgggg
     5641 aaatatttct caaaaggcct ctttaaaaga acctgcccgc ctctttgagc cgaagccggt
     5701 aaaccaaaaa agacctttaa cataattcca tcaggaacta tgctaaaagt ctcgcttccc
     5761 tttttaaagg gatataaaac caggcacata ataaataatc ttgccggttg ttggctctat
     5821 atttcaagct actccctctt aacactttaa tggtatcatg gagctgattt tttgtcaatt
     5881 ttatttaagt atattagtac atcagcacat aatactttca gccagcctga acatcactga
     5941 ttgcagctgc ttctgctgag gctatgctgt gatcttctgc ataatcactt ccggcagttt
     6001 cattaattat actgcccagt tgagtctcat acttttttaa agtgcttgat atggatttct
     6061 tgagcatacg gatttcttcc ttgatgtcaa caagtttctc tttctcatgc tctgtcatct
     6121 cctgtatctt attcttctca tctagtgcct ttattctggc atcttcaatc attagttgag
     6181 ctttttcctg agcttttatt aagacttccg caattttctc tctttcaatg ttaatttgtt
     6241 cttccctatt agcaagctct tcatatttgg ttttcaagtc tctattttca ctttttaaat
     6301 ttgcaatttc catttccagc tcttttaatt tgctttcaaa atcctgaagc agattttcaa
     6361 tatatgaatt aacatcatct ttgttaaaac ccaaaaatgc atttttaaaa tgtttctcac
     6421 caggcatata tcaaaacctc cgtagcttgt gttatttata tctattcaat atattaatat
     6481 aaaaatcctt cttttttaaa aaaatttgag gaaatgaaaa gctcactatt tttaatgagc
     6541 atggagctca tgtattgtga atgaaaaacg gagggctgtt ccagcccccc actaacctgc
     6601 ttttaaagat atttatcgat tattgttttg aattcctgag caggctttgc tccaacaatt
     6661 ctttcaacca ttttgccatc tttaaataaa gctattgtag gtatgctcat tattctaaat
     6721 ttggctgcaa gttcactttc attatcaaca tttaattttc caataacaac ctttccatcg
     6781 taatcatctg caagcttttc tattacaggg gctaccgcgc ggcatggtcc gcaccattcg
     6841 gcccagaaat ctacaagaac caatttacct gaatcgatta cctccgtatc aaaattactt
     6901 cctgtgagtt ccaatacttt ttcacttgcc atacatatca ccctttctat aaaaatgcaa
     6961 taatatagac taatactatt caactgttat tatttttacc cttgaatcct aataacaaac
     7021 aaattctttt tcttcttaat cctttgctct ataatatgat tccacagaaa caatgatttt
     7081 accgcagaga cttagattta cttattcaat aaatttaagg aatttattaa ttgtattaac
     7141 aagctcatca aaggctttag ctcttttatc tgaatcttca gaatcaaaag cagatttaag
     7201 gcatgtcttt gagtaattct caaggataat tacccctact ttatttatag cagcccgcac
     7261 tgccgccacc tgcgttaaca catccataca atatttatcc tcatttacca tcctttgtat
     7321 accttttacc tgtccttcta ttctattaag ccgtttcata atattgagct tttgcaggtc
     7381 catctgagta ttataaactt taccattatt atgctccgag ttattattat ttatcttttt
     7441 atgatttgta ttggaagtat tctcaatatt gggaacaacc gagttattta tattatctga
     7501 atcatacttc atagttattt ctccctatag ttatttctcc ttgtttaatt tgtaattcag
     7561 ccatatacct tgtatgggta tatatttatt atatattttt tatggaaaat agggaagtat
     7621 aaaatatgta ccctatgcat tctaaaaatt tttatctaaa aattatactc taaaaattat
     7681 tcatatatag tataatcttt ggtaaaataa tataaagtaa ttaagcagat tataataaaa
     7741 taacaaagca taaaccatta tatgaaaagt ataaaactaa gactacttta ctatgtttcc
     7801 aaattggcag agtagtacta ataataattt aatatataag tatacaaaaa taacaacaaa
     7861 aatagtaact ggaggtagag aagcaaatat gtatattaca ggcaaaataa cagttaaccc
     7921 tgtacttccc gaaaaacttg ccaggttaaa ggatattgca tacaatttat ggtggtcatg
     7981 gaacgttgaa gcactggatt tgtttattga tattgatgct gagttatggg aaagtgttcg
     8041 ccataatccg gtcagatttc tacatgaagt aagttgcagc aaattggatg cagcgcagaa
     8101 taatgaaaat tatatggata agtataatta cattattgag aaattcgatt catatattaa
     8161 tgataaaaac acttggttca ataaaaacta cccggaatta aacaaccacg gggtagctta
     8221 cttttctgca gaatatgggc taaatgaaat acttcctgta tattccggcg gacttggtgt
     8281 tttatccgga gaccattgca aatctgctag cgatttgggt attcccttta ctgcaatcgg
     8341 tttattctac aagcaggggt attttagtca gcgcattaat agagaagggt ggcaggaaac
     8401 agcctttaca gatcttaata ttacaaggct tcctctcaat cctgttttaa attctacagg
     8461 gcagcatgta atgattagcg tggagttacc tggaagggtt gtttatgcaa aaatatggga
     8521 agtaaaggtt ggacgtatat cgttatactt aatggatagt aatgtagaac agaataatga
     8581 aactgacagg tggctgacct ccagattgta tggaggtgat caggaaacaa gaatacagca
     8641 ggagatactt cttggaatag gaggtataag agtccttgaa gcattagaaa taagcccttc
     8701 tgcatatcat atgaatgagg gtcattcagc atttcttggt cttgaaatta tgagaaagct
     8761 tatacaggaa aaaggtcttt catttaaagt tgctaaagag gtggctgctt cttgtattgt
     8821 gtttactact catactcccg ttcctgcagg caatgatgtt ttcccgttgc acatgatgga
     8881 cagatatttc agcaactttt ggaataaact gggtataagc aggcatgagt tgctggatct
     8941 tggcctgaaa gtaaatgata gtcagaattt taatatgaca gtacttgcat taactcttgc
     9001 aggacgtaaa aatggggtaa gcgagctgca tggcgcagtt accagaaata tattcagtaa
     9061 tttgtggccg ggaatacctg aagatgaaat tccagtatct catgttacta atggaattca
     9121 cactcttaca tggcttgcag gagagttgaa tgatttatac ggtaaatatc ttaaagtggg
     9181 gtggcaggag aatatttcca ggaaagaaac ctggcaggat atagataaaa tacctgatga
     9241 acttctatgg agtactcatg taaaattaaa gaaggaacta atagaatttt taagagttaa
     9301 gattaaaaat cggagaatat ctaacgggga atcatcagat agtataagag aagcggggaa
     9361 tctgctcgat ccaaatattc tgactattgg ctttgcaaga agatttgcta cttataaaag
     9421 agcaaatctg gtatttaggg atatagaacg gataaaaagg attatcaata accctgacat
     9481 gcccgtgcaa ataatatttg caggcaaggc tcacccagcg gacagaccgg ctcacgaagt
     9541 cattaagaat attaatgata ttgcaggaca ggaaggtttc aaaggtaaaa taatcctcat
     9601 agaagattat aatatggaag ttgcacgaaa actggtacat ggagttgatt tatggcttaa
     9661 taaccccaga aggcctttgg aagccagcgg aaccagtggg caaaaggcat gcataaatgg
     9721 cgtaattaat ttcagcgttc tggatggctg gtggtgtgaa ggattcaatg ggagcaatgg
     9781 ctggatcata ggtgatgata ccttctatga taatgagtat tatcaggata atgctgatag
     9841 cgtttctata tatggaactc ttgaaaaggt gatagcacct ctattttaca gtaaagataa
     9901 caatggtgtt ccagcaggtt gggtgagggt aatgaaagaa tctataaaat ctctgattta
     9961 tcgtttcagc accgatagaa tgcttgaaga ttataccaat actatgtatg caccttctat
    10021 ggaaaggatg gagaaattca tatcgggatc cttcagttat tctaaaaaac ttgctgaatg
    10081 gaaatcattg attgagaaag actggccaca aataacaatc cgttcagata aaaccaaaaa
    10141 tcagcttaaa gaatataatg tgtcttcctg tgaaaagctg acccttgctg taactgttga
    10201 ccttggtgac atagacccgt catatgtcaa agtggagtct tattatgggt atattaaaaa
    10261 tagtaatcat attgaagacc ctaaaataat tgagatgaaa cctgtagaaa aaaacgggcc
    10321 atcttcatgg ctgtataccg ccgagatcag attaagtgat ggtggtgaat atgcttacac
    10381 atttagggtt ataccctacc atcccgattt aatcagtaaa tttgaaatgg gattaataag
    10441 atgggttgtg agctagtgaa atttagtaaa ccccttgtaa tttttgaaga ctgcaagtat
    10501 aatataattt aataatttga gcgagtttta ttatgttatc gtggtggctg gttttcaatc
    10561 aaataagcct ctgctgcggt atagatttag ataatatatt taaaaacagt gaacgggaag
    10621 agtacatctg gcatttttga agagagccgg cgcctggtga aagtccggta aaatgtaata
    10681 tggaaggcag cccgggagtt gcttaccgag aaatagagaa cctgtctcta aagtagggaa
    10741 agacgtaaat cctcacgtta cagaggatag gtggaataca ttaatgttac tgcacttttt
    10801 tgcaggcact gttgagtatc cgccgaaaga gaggacactt acaacagtgt caaatagggt
    10861 ggtaccgcga aaaaaacttt cgtcccttag cgggggcgga agttttttta tataagtaat
    10921 agaaaataat tcaatgggag gttaattatg gaaaataaaa acctggagtt tactgaagaa
    10981 atactggaga tactggagaa caattgcaag tattcagaag aacaaattgc tgttatgtta
    11041 aacagagatg tagagtacgt tagagaagca ataaagaaac tggaagatga aaacattatt
    11101 gcaggctata gaacagtggt agattgggaa aagatgccta aagaaacagt tacagcttta
    11161 attgaagtaa aggttacacc tcaaagaggt gaaggctttg ataaagttgc tgaaagaatt
    11221 ttccggtatc cagaagtaaa agcttgctat cttatgtccg ggggatttga tcttactgta
    11281 ataatagagg gaaagacatt aaaggaagtt gctttctttg ttgctgaaaa actggcgcct
    11341 cttgattctg tcttaagtac ctctacccat tttgtcctaa aaaaatataa ggataatggt
    11401 acaattttcg agaaagatga aaaggataac agggaggttg ttattctatg aatataactg
    11461 aaatgatttc ccctttggtt aggaatatac ctccttcagg aatcaggaaa tattttgata
    11521 tgattaatga gatggaagat gttatctcat tgggtatagg tgaaccggat tttgttaccc
    11581 catggaatat aagagaagca ggtatatatt ctctagaaaa aggagataca cactattcac
    11641 ccaattcggg atatgccgag cttagaaatg agatatcaaa gtatttaaac aggagatttg
    11701 aagtccaata caacccaaag aacgagatga ttgtaactgt cggtgggagt gaaggaattg
    11761 acatagctct cagagccctt gtcagtgcgg gcgatgaggt tataattccc gagccttgct
    11821 ttgtagctta taaaccctgt actatttttg caggagcaac acctgttact ataaacctcc
    11881 gtgaagagga ccagttcaag ctgacacctg agcttttgaa aaaagctatt actccaaaaa
    11941 caaaggtact gattcttcct tttcctaata atcccacagg tgctataatg accagggaag
    12001 acctggaggg tatagttgat gttctaaaga aaaaagatat aattgtaata tcagatgaaa
    12061 tatatgctga actgaactat gaaggtaagc atgtatccat tgcgtctttc ccggaaatga
    12121 gggataaaac tcttattatt aatgggttct ctaaagcttt tgcaatgacc ggatggcggc
    12181 tggggtatgc atgcggtcat cctgatttga ttgcagccat gttgaaaata catcagtatg
    12241 ctataatgtc ttctcctaca actgctcaga aagctgcagt tgaagctttg aaaaatggtg
    12301 ataaggatgt atacacaatg gtccgggaat acaacagaag aagaagggtg atggtggctg
    12361 gtttcagaaa agcaggatat aattgtttcg aaccactggg agccttttat gtattcccat
    12421 gtataaagtc atcaggaatg agctccgaat tgttttgtga aaacctgctt attgaagaaa
    12481 aagttctggt tgtgcctggt acagcttttg gccggtgcgg cgaaggattt gtgagagcca
    12541 cttatgcaaa ttctatggaa aatattattg aagctttaaa gagaattcag aattttgcag
    12601 gaaagcatat aaagtgatat aatatcaaaa taaagtaaat attataaaga gatttacaga
    12661 tttacagatt tacaggttac attataattg gattttgagg ttaaacgggg tgcctaaaat
    12721 gggatttgca ctaaacacaa aaaaactcag ggaacaaaat aaattatcac agaaagaaat
    12781 agccgattat ctgggtgtta caagacaggc ggttgcttcc tatgaaagtg ggaagagaga
    12841 gccggactat gggacattaa taaaattgtc ggatttttat ggagtttctg ttgattcctt
    12901 gttgggcaga tctcaatgca aggatataaa tgtattgact atagccaaaa atattgtatt
    12961 aataaaaggg gattttaatt atgaagaatt ttccagggat attttccgga aaacaggggt
    13021 atgcatttta gagaacatgc ttaaacttta tgctgaaggg gaaaaaatac ctgctatagg
    13081 tacaataaaa gttttggcta agtatgcctc ggtacatgaa tccttttttt acaagtttaa
    13141 taccttggag gattatgcaa acgaaaaagc tttatatgat gcaaaaactt tatgtaatga
    13201 aaaaacctcg tatgacattt tttcatgcaa ccttgatttt atggaccctc aactggccgg
    13261 gtgggtgcag gatgaaaaaa acatgcacta tataatgctg gctaaggaaa ttcaagatac
    13321 taatctgcct ttggaaagtg taaagtcaat tttgagtgtt ttaatttctg ttcaaacagt
    13381 aacggagaat agtaaaaaac aaaatgttta gtgtaagtgt gtgtggtaag tggtaaaata
    13441 caaataaata ttggtaatat tataatttga aaggaatgat actatgctgg aattggagca
    13501 gtataaattg gagattagag atttgggaga agagattacg gaaatgaggg attcactttg
    13561 acctcccagt tttgaaaaat gaacttgatg aattggaaca gaaatctgcg gaacctgaat
    13621 tttggaatga catggaaaaa tcgcagaaaa tactacaaag aataaaaaca ttaagagata
    13681 agctggaaac gtttggacat ttagaaagta ccctggaaga tttaaagaca ttaaatgaat
    13741 tgggtattga ggaagatgat gaaagtgtaa tagatgaagt aaaagaaggt attgaggaat
    13801 taaaaggtaa aatagccaag ctaagaactg aaacactttt aacaggacca tatgataaaa
    13861 ataatgcaat tttgagcatg catgccggag cgggaggtac tgaagctcag gactgggttc
    13921 aaatgctttt aaggatgtat acacggtggg gtgaaagtaa gggatatact gtaaaaattc
    13981 ttgattatct tgatggtgat gaggcgggtg taaaaagtgt tacgatacat gtgatcggtg
    14041 aaaatgccta tggatatttg aaatctgaaa agggagttca cagattggta aggatatctc
    14101 cttttgatgc ctcaggccgc cgccacacat cttttgcttc gattgatgta atgcctgagc
    14161 ttgatgatga tattgagatt gaaataaacc ctgatgactt gagggtagat acttaccgtt
    14221 caagtggagc agggggacag catgttaata agacagagtc agccataagg attacccaca
    14281 ttccgacggg cattgttgta tcatgtcaaa atgaacgttc ccaatttcaa aacagggata
    14341 cagcaatgaa aatgttgaga gctaaactct ttgaattgaa agaacaggaa caaaagcaaa
    14401 aaattgaggg aataaaaggt gtacagatgg aaatcgcctg gggaagccag ataaggtcat
    14461 atgtattttg tccgtatact cttgttaaag accacagaac caactatgaa gaaggaaatg
    14521 taaatgccgt gatggatggg gagatagatg aatttattaa tgcctatctt tcagaagtgg
    14581 gaagtgagaa gtgagaagtg gatagggtat agtggattgt gagaggtata cttttaatgt
    14641 tatataatag attttctgag tatttgaaaa aaagatataa tgcaaaagtt tacaagctgc
    14701 ctgtaaatat tcctgtctca tgtcccaata gggatggaag ggttagcagt aatgggtgta
    14761 ttttttgcgg cgaggaaggg gcaggttttg aaaacctgcc tgctgcttta ggcgtaaaag
    14821 aacagataag aataaatgcg gagcatattg cagcaaagta taaacctgag aagtatatta
    14881 tctacttcca gaattactcc aacacctatt tgcccataga accttttaaa aagtatatta
    14941 ttgaagcatg tgttgaaaat gctgcagcaa tttacatatc tacacgtcct gactgcataa
    15001 acgaagagca gctggacttt ctcaggaaca taaagaatac gcagaaaatt gatattttaa
    15061 tagaattggg cctgcagacg gtaaactata agacactaaa atttctgaac cgcggacata
    15121 ctttagccga gttcattgat gcagtacttt ccataaataa atatggactt gaatcatgtg
    15181 cccattatat aattgatctt cctatggata gtatggagga tgttatggag ggcgcaaaaa
    15241 tactttcagc cttaaaggtg aatcaagtaa aatgtcattc attatatatt ctcaaagata
    15301 cggtacttgg tgaaatgtat gtaaaacaac aaatcatgcc tgtgactctt gaggagtata
    15361 ttgagagaac aattaccttc ctggagtacc tgaatcctga aatagtaatt cagaggcttc
    15421 ttggacgtgc acctgaagat cgaactctgt tttgcaactg gggtatgagc tggtggaaaa
    15481 tcagagatct ggttgaagca aaaatgataa aggaagaaag ataccagggt aaatatttta
    15541 attaccttgg gggaatcatt tgattagaaa ggtacataga ttagttctac tatgccaatt
    15601 tggatgcgat atcttcaatg cgctttccat tggcaacata ataaagtagt attagaagat
    15661 atacagacat gaaaggggaa aattacgaat gaaaaatgaa atgctattga ttgtaagctt
    15721 taatggcaga ataagccaga ctattgccag aatggtaaga gaattgggtg tatacactga
    15781 agtgatacct tacaatgcgt ctatagataa aattaaagct gcgggacccc ggggtgttat
    15841 tataacgaca tctcaaaatc aaggcttgga cgaaaggctt gatttcataa aaaaagaaat
    15901 ttctgaaata gatatacctt ttttggaaat tgcagcacct gataataact tcttaaggag
    15961 ttttatacat gatacctgca aatgttccgg cggctgggat atgaaatctt ttgtagctca
    16021 gtctatcagg aatataaaag agaaggtggg agataagaag gtattgtgcg ctctttccgg
    16081 tggagtagat tcctcagttt gtgccgtgtt ggttaacgaa gcaataggaa aacaactgac
    16141 atgtattttt gttgaccacg gcttactgag aaaatatgaa gtagagcagg tagaaaagct
    16201 atttagagaa aagtatgata ttaaccttgt taaaatagat gcaacagata gattccttgg
    16261 aaaattggat ggggttactg atcctgaaaa aaagagaaaa attattgggg aagaatttat
    16321 aagagttttt gaagaagaag caaaaaagat aggcgctatt gattatcttg tacaaggaac
    16381 tatttacccc gatattattg aaagcggcac agaaaatacc ggtatggtaa aaagtcatca
    16441 taatgtaggg ggactccccg aaaacattga gttcaatgag atattggaac cgctgaagta
    16501 tttatttaag gacgaagtca gaaaagcagg acttgaactt ggtattccgg aaagtattgt
    16561 ctggagacag cctttcccag gtcctggact tggtgtcaga gtaattgggg aaattacaaa
    16621 agaaaagctt gatatactaa gagatgcaga ttatatattc agggaagaaa ttgcggcatc
    16681 aggacttgat aaggagataa accaatattt tgctgttatt acagatatgc gcagtgtagg
    16741 cgtaaaagaa ggcaaaagga cctatgatta tacagttgct ctccgtgcag taaaaaccac
    16801 agattttatg actgctgaat gggtcagaat tccttatgag attcttggaa aggtctcaga
    16861 gaggattgtg aatgaagtag gccacattaa cagagttgtt tatgatataa ccggtaaacc
    16921 tcctgcgaca attgagtggg aatgatttga aggacagtaa aaagccagta actacgcagt
    16981 tttgcgggct ttcaaacact cctgtggcaa cgttttggca acactaatta catttatcca
    17041 gatgaaaaga gctaactgat tataagtgtc agttagctct tttttattag catgaaaacc
    17101 gaaaaatcga tgattagttt ttgcacccgt tcttatcgca tgtgactgct tgggtagtgg
    17161 aaacccaatc gcctggatta tcatctattc tcgctccgca gcctgtataa ttgccttttc
    17221 tgatatggat cttaccgttt tcggatttct ttgtatcgtt tccaatgtac ttgatgttca
    17281 atattattca cctcctttat ctgtaatctt tgcaattttc ttatgtttcg gctttcaatg
    17341 ctacttacag tatttttggt cattcatcca tatcagtatt tttcttttcg gatcgtaaag
    17401 catcaagtcg ttgctttgcc cgcttggctt ctacttttgg atagttataa cgccagtcat
    17461 catattcatc cttggtgatt tcacctcgtt tgaacttatc gtactctttc tgccatgtac
    17521 taaacatttc atacatagat agatagggag tacctttaga tctatcaagt gccaggcaaa
    17581 tctcaccatc aattattttt atttttaaac catagaggtc ttctagtgca aatagcgtgt
    17641 gcattacacc tatatcagaa tcaatattag gcacggttaa agcctcaggg ctgatttcca
    17701 aagcggctgc aagcgaattt atcagatttc cttttggagt cctggtaccc gcttcatact
    17761 gtgccatgcg aacatcagcg gtcttctcat caaagccagc agcaatgcca aggtattttt
    17821 gtgtcatacc ccgtaagttg cggataaacc gaattctttc accaatagac atacaatatc
    17881 cccaatccta tacaaatcca attctgataa gcataataac atatttgttt aagaatagtc
    17941 aagataatat aaacaaaaaa gtttaattat tttctcgaaa acctcttgac ataaacaaat
    18001 atgttaagta taatcggtat gaagttaaac aaaaaggttt aagtttatac aattaaatga
    18061 ccgtccaagc agctatcttt taggtaagta tgccatgacc ttccgagtga agcgagcgtg
    18121 ccaataaaga gaaaacaata cagcgccaag caaggttgcc tattaggaac tgtgagtggg
    18181 gagaacggca gaatttaggt aggggggaat ttgatgagta acacttttat gaaggttgat
    18241 gaagtggctg cagagcttgg ggtatccaag tcctatgctt ataagattgt gcaaaagttg
    18301 aatgaagaaa tgaagaaaat gggatatctg acgatttcgg gccgagttaa caaacagtat
    18361 tttatgaaaa agacgtgtta taacgataca aaaaagaaag aatgtgatga acatgccagt
    18421 ttataaagat aaaaatggaa cttggagagc ggtatatcga tataaggact ggaaaggtga
    18481 gcataagcaa acacaacgcc gtggatttat attaaaaaaa gatgcacagg cgtgggaacg
    18541 ggagcagcta cagaaaatcc agggtgaact ggatatgact tttgaaagct ttgtggaaac
    18601 ttatacagct gatgtgaaga atcgaatgaa agaaaacacc tgggaaacca aggaacatat
    18661 tatccgcaca aagctgactc cgtactttgg caagagaaaa ttgtgcgata ttcagccaaa
    18721 agaggtaata gcttggcaga atgagatgat aaattataag gatgaaaacg gaaaattgta
    18781 ctcaccagta tatcttaaaa ctcttcacaa tcagcttagc tgcattttta atcatgcagt
    18841 caagtattat ggcctatcgc aaaacccggc agcaaaagtc ggcaatatgg gcagagccag
    18901 aaacagagaa atgctgttct ggactaaaga cgaatatcta aaatttgcag atgcaatgat
    18961 ggataagccg atgtccttct acgcatttga attactctat tggtgcggta tccgccaggg
    19021 cgaattatta gcgctgacgc cgagggatat agactttgaa aagaaaacag tatctatcac
    19081 aaaatcctat cagcggataa agggcaggga tgttattact acaccaaaaa caccaaaaag
    19141 caatcgtgtc attaaaatgc cgcagttttt atgtgatgaa attcaggaat atatcagtat
    19201 gttatatggt acagaaccaa ctgaccgact ttttccggtc accaagtatt atcttcacca
    19261 tgaaatggaa cgtggttgca aggaaaccgg ggttaagaaa attcgtattc atgatcttcg
    19321 tcattctcat atatccttgt taattgatat gggcttttca gcaattgcaa ttgccgaccg
    19381 tgttggccat gaaagtatag aaattacata caactatgct catttgttcc cgtcaaggca
    19441 gacagaaatg gcagaacgat tggactttga acgtacagaa ggagaggagg ggtaactgat
    19501 gtcattaaag aatttagatc gcaaaaaccg ttggagaaac aagacggttg cattccgggt
    19561 atctccggaa gaagacagac agattgaaac agctgtccgg ctttctggtt tgacaaagca
    19621 ggattacatc attcggcggt tgcaggacag agaaatcgtt gttgttggca atccgagggt
    19681 atataaagcg cttcgtaatc agcttgctgc ggtacttgag gaattaaagc gtattagtat
    19741 tggtgaacag attgacgatg agcttacaga tacaatccgg ttgatcgccg taacccttgg
    19801 cggattaaag ggggagtaat aatggcagca aaagaaaaag cgactgccat agcatcgtct
    19861 gcgccaacag acgaaaagca gtcacttaac ctatgtaata acattattat gtctgagaat
    19921 tcgaaaaaaa tcaatcaaaa aaatgaaaat accagaatac agtttctttt tcggcagatg
    19981 aataatcctg attattttaa tactgtctcg ctaacagagc tatatgatac ggtttatcag
    20041 ccaaaaacgc aggtgattga caatttgcta tatgcaggag tctatctctt tgctggtgct
    20101 ccaaaggtag gtaaatcatt ctttatggcg caaatcggat atcatgtgag caagggtatt
    20161 cctttatgga attatactgt tcacaaaggc gtttccctct atcttgcgtt agaagatgac
    20221 tatgcaaggc ttcaaaaacg attatccaaa atgtttggta tggacggaac agacgacttt
    20281 cactttgtca ctaatgctaa aaatttaagc caaggacttg aaagtcagct agatacattt
    20341 attcaaggac accctgatac gaggctcatt ataattgata ccttgcaaaa aatccgtgaa
    20401 gccggtgggg aaaagtttag ctatgccagt gactatgaaa tcgtgacaaa actaaaacag
    20461 tttaatgcag agcataatgt ttgtattctt ctcgtacacc acacccgaaa acaaaacgca
    20521 gatgattgct ttgatacgat atccggtacg aatgggttat taggtgcagc agacggtgct
    20581 tttcttatgc aaaaggaaaa gcgtactgac aatatagcag tgcttgattt ggtgggcaga
    20641 gatcagcagg atcagcggct ccatttacaa tttgatcgtg agcgctgtgt ttggcaattg
    20701 ataaaagcgg aaactgagct ttggaaagaa ccgcccgatc ccattctttc tttgatttct
    20761 ggcttagtaa ataaagaagc accagagtgg attggcagcg caacagaact tatagaaaga
    20821 cttaaacttg agatgcaacc taatgtactg acaaggaggc taaatgtcgg agcggactgg
    20881 ctttggaatg agtacggaat cagctatgag aacacccgca gtcataccgg acgacaggtt
    20941 aagtttactc tgaacggtaa gtaagcgtga cgcttgtgac gattgtgacg atgtttttgg
    21001 tgacgatatt actgtaaaaa ttatcgtcac atcgtcgcgg cataagttta gggaaatggt
    21061 gcggccgccc tttttaaagg gcgatgagcg ctggtgggtt gcccgcagac aacacggggc
    21121 accgtcccga gccgtcaccg gacggcctat ccccctcgga gagcgcaatg cgcttttgta
    21181 catgggcaat ccggtggatt gcacgtgtca aaagtgcttt tgcgttactt ttgacaaaag
    21241 taacaaaacc cggcattcgc cgataccagc cgagaaagaa aggtggttgt atgaaaagga
    21301 caatcagcat tatggttggc aaaggttcga taaatcataa cagtaggaaa tttcatgcgg
    21361 aaaatactga cccagaaaga agcaatttaa accacaccta ctgtaacata cccattaaac
    21421 aaatctacca tgaacttttt gatgaagcag taaaaaggta taacgaaaaa caaactcgta
    21481 aggacagatg tattgatgac tactatgaga aaatcagaag cggtaaacag gaaaagccat
    21541 tccatgaaat tgttgtgcag ataggtaata aggatgatat gaatgctaaa acggaagagg
    21601 ggcaccttgc tgtaaggata cttaatgaat atatgcagga gtttcagaat agaaaccaaa
    21661 atttaaaggt gttttcggct catcttcaca tggacgaggc cactccacat ctgcatattg
    21721 attttgtacc atttactact ggcagcaagc gtggtcttga t
//
LOCUS       DAOSDD010000052        21541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1059_length_21541_cov_5.108443, whole genome shotgun sequence.
ACCESSION   DAOSDD010000052 DAOSDD010000000
VERSION     DAOSDD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21541
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1059_length_21541_cov_5.108443"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PK733_06655"
     CDS             complement(<1..626)
                     /locus_tag="PK733_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOJ10258.1"
                     /translation="MGKIIGIDIGGSTTKIIGFDNKQMFSPMIVKAADPISSVYGAFG
                     KFLSINKLKLDNIERIMVTGVGSSFITDNLFNIPTGKVDEFRAIGMGGLFISKLEKAI
                     IVSMGTGTALVTADNTGVRHIGGTGVGGGTLLGLSNRMLNVRSFDDLIETAQGGNLAN
                     IDLTIGDISTETAVGSLPPETTASNFGKISDLATKADLALGIINLVFQT"
     gene            769..1980
                     /gene="hydF"
                     /locus_tag="PK733_06660"
     CDS             769..1980
                     /gene="hydF"
                     /locus_tag="PK733_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOJ10259.1"
                     /translation="MKEKIMMNDTPRSDRLHIALFGRRNTGKSSLINAITGQEAAVVS
                     NVAGTTTDPVYKSMEVLPIGPVVFIDTPGIDDAGELGRLRTEKTREVLRKTDVALLVI
                     GADMLWGKYEVELAEEFRKRSIPFIIVANQVDKSAKQSEIELENSTVTTIVKTSALTG
                     EGIDKLIEELVKVKKEDDQRLHLIDDIIKEGDLAILVTPIDKGAPKGRLILPQQQVLR
                     DILDNNAMAFVVQDNRLEEAINRLSSPPALVITDSQIFGKVGNVVPEGIPLTSFSIIY
                     ARQKGDLKKFYEAVKEIENLEDGDLILIAEGCTHHRKDDDIGTVKIPSLLMKKTGKHL
                     KFNHVSGGFFEEDLSKYKMVIHCGACMLNKKEMEYRQEHSQSSGVPMINYGIILAYLN
                     GILERALEPFK"
     gene            2202..4523
                     /locus_tag="PK733_06665"
     CDS             2202..4523
                     /locus_tag="PK733_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10260.1"
                     /translation="MRSLKRNTSILHMYFTSYIAIFAIPLVILGIVLYSHAIIELQNE
                     VERYTQDKVIRMKNTLDARLLETKKTALKISSDENIKPHMVNLNDYRSKNAIDQLNRY
                     WDKNLIDNLFVCFRNEKYLYGVAGKTSFDTFFANTIGIDYNTSISFANALQKTDKSMF
                     LGISGIPSGSSKNIDNLICLYPLPLGGVSSYGVLGIIINQGTIENLAGDILGNSKGSV
                     FIYNEDMQRIFTIGDKDFFPSERVIEITGGNYSAGVSKSKFEGRNYSVIKTVSEESGF
                     HFIVIMSPESYLNRVIIMRKQVMVIIFGVILTGILLAYVFSRKNYKPIKVLFEKMNNY
                     TSLSEQKEINEPSIINKKVNEIEGIEKMIKNIVDKNYGLTEQLNHHQSFVRDQILLML
                     LKGQLNEKENIKDLLALYNMKFDYLYSYVSVISIQKVKEYETTAAELSLYIKGVIDSK
                     LKNKQFYWIDTVQGDFVLLVNTTYENCSKIMKVNLLKDIQIFLQNNLGLKINIGVGRV
                     YDNISQISISFMEALSTLDYKLLIGYGNIIFFDEMVSSDTMVYWYPVEEQIRLSQCIK
                     QGNIYIFRDIIDDIIDSIKEKHLPIGITRTICFGMINTIIEVLNEFNIKDFDSDVEEL
                     TTFTSLDDLKNKMENVVYKICKYISEKKESNNETLKIAIIEYIHENYTNNMFNLDMVA
                     DKFNISSKYLSRFFKQQIGFNFVDYMKELRINYSKKLLSETNLLIKDIVEQVGYSDVA
                     SFTRTFRQMEGITPGRFREMALNTVTQQEKNSF"
     gene            4706..5659
                     /locus_tag="PK733_06670"
     CDS             4706..5659
                     /locus_tag="PK733_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10261.1"
                     /translation="MKSEAIPEVAISVKKSGSSTLKKMLSTYELYLFILPTVIYYIIF
                     HYAPLYGIQLAFKDFIATEGIWGSPWVGLKHFKLFFSSHYFWELLRNTVGISLYALVA
                     GFPMPIIFALMLNEISSKRYKKIVQTVAYAPHFISTVVMVGMLYIFLHPVYGIANSLI
                     KVFGVQAVDFLNRPEYFKSIYVWSGIWQSTGWNSVIYFAALSSLDPEQHEAAIIDGAT
                     RLQRIWYINIPVIIPTAVILLILSAGGIMSVGFEKIYLMQTPLNANTSEVISTYVYKV
                     GLQGARFSFTTAIGFFDSIINFILLIIVNRVAKSLSSTSLW"
     gene            5675..6565
                     /locus_tag="PK733_06675"
     CDS             5675..6565
                     /locus_tag="PK733_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10262.1"
                     /translation="MKKSKDDIIFETINTILITIFFVIVLYPLLFVLSASISDPSLVS
                     QGKIILLPKGINFNGYKRVLAYSDIWLGYRNTIFYAITGTLISLFLTLTSAFSLSRKD
                     FIGKNIFVVFFTVTMFFSGGLIPTYMVIKSLGMRNTVFAQLLPGAVSFMNIIVVRTFY
                     QTSIPDSLQEAASIDGCSIFRLFVSIILPLSKPIIAVMGLFYGVAQWNSYFKALIYIS
                     DRKIFPLQLILREILVVNQINMDLLMNEGELEVIHKRVELATLLKYSVIVVSTLPVIA
                     AYPFLQKYFIKGVMVGAIKG"
     gene            6731..8362
                     /locus_tag="PK733_06680"
     CDS             6731..8362
                     /locus_tag="PK733_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10263.1"
                     /translation="MLKKPFAKILSLIVVMLVLVSSFTGCVKSSGTDLSSEQTKKPDT
                     TEKQKPTEPAPNALVSDKKITLKVMSQRDAMGGPWEDLRLFKDLEEMTNVHFEFDVVF
                     EGFQEKKNLALATGDLPDIFLGALNVDDEENYGPQGMLIDLTELIDKHGKNMKATFSK
                     YPTLFKTMTASDGKIYALGQILVTTTMGSYKPVINSTWLKNLNLKVPETTTDLYNVLK
                     EFKTKDPNQNGKSDEIPLGDIGFNHLNGMLLTAFGTMAQDISVKDGKVVFTPMTDSFK
                     HYLEYINKLYKEELIDPMFISHSMDEFLAKTKQNLYGVLGNIRQSDWTQFQILNPLTS
                     EYNSEKFSSSTDSLYSAGRFAITKNNKYPVESIKWADLFFTELDKGVNGVTGAALWIG
                     RRGVDWDFTDDKKAVKYLFTPDEGTTEIQTLLKKVSPSQTGFVGELIMNVPFESPYLK
                     WVGENNKNNVFPYLRNERLFPAGARFTKEEQEKISILKTDITKFVEQERAKFATGDNS
                     FDNWNNYIKSLESMKVDEYIKLHQQAYDRFMKGGK"
     gene            8627..9454
                     /gene="zupT"
                     /locus_tag="PK733_06685"
     CDS             8627..9454
                     /gene="zupT"
                     /locus_tag="PK733_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOJ10264.1"
                     /translation="MRLNVSTSAILYAFGLTTLAGISTGIGGLFTIICKKTNKKFLSV
                     ILGFSAGVMIYLSFMEILHEAMNYLTNVLGETGGSWAAIAAFFGGVFLIAIIDKLIPS
                     KDNPHESHKIEEKHTCDSSYNNWKLLRTGLLTAVAITIHNFPEGIASFISGLKEPSLG
                     IAIAIAIAIHNIPEGIAVSIPIYCATGSKRKAFLLSMASGLAEPLGALVGYLILQPFL
                     NDLVFGILFAAVAGIMVFISFDELLPTAEEYGEHHLSILGLIGGMALMAVSLILFIK"
     gene            9907..10038
                     /locus_tag="PK733_06690"
     CDS             9907..10038
                     /locus_tag="PK733_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10265.1"
                     /translation="MTENRGGFGCAGFGNEWIWWIIIIIIVICLFCPGIFGGFGCRE"
     gene            10374..12767
                     /locus_tag="PK733_06695"
     CDS             10374..12767
                     /locus_tag="PK733_06695"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ10266.1"
                     /translation="MRKQRLIINGMTCTACAKAVERSVGKLEGVNKAIVNFATEKLDI
                     EFDENKVGINNILETVKKAGYRAEEEAEESIKARVVYDTSKIKTSQIKGAVSKAGYKA
                     LEIERVEKTDADTDRKERERKLLWTRFIVALSFSIPLVYIAMGSMLGLPVPHIISPEK
                     YPVNFAIMQLALTLPIAAAGYRFYTVGIKQLLKGHPNMDSLIAIGTGAAIIYGLYAIA
                     RILEGELLYVHNLYFETAGMIITLVMLGKYLETVAKGKTSEAIKRLMGLTPKTAIIIH
                     DGREIVIPVEEVETGDIIIVKPGEKIPVDGEVVEGHTSVDESMLTGESIPVEKTVGDK
                     VVGATINKNGMIKFRATKVGKDTVLAQIIKLVEDAQSSRAPIAKMADVISGYFVPVVI
                     STALLASILWYISGQPPVFALTIFISVLVIACPCALGLATPTAIMVGTGKGAEYGVLV
                     KSGEALEASHKIHTVVLDKTGTITEGKPTVTDIIVLGDITQTDLLQLAASSEISSEHP
                     LGEAIVNMAKKEGLDFLNTDSFEAIPGHGIKAEIQGKLVLLGNNKLMDDRGIDFNVKI
                     AETDTRLGQEGLGRLAQEEFDRLAQEGKTPMFVAFDNKPAGIIAVADVVKESSKPAIE
                     ALHKMGIDVVMITGDNKRTAQAIARQIGIDRVLAEVLPEDKANEVKKLQQEGKKVAMV
                     GDGINDAPALAQADVGIAIGSGTDVAMESADIVLMRSDLMDVSVAIQLAKKTMRNIKQ
                     NLFWAFLYNSAGIPIAAGLLYYFGGTLLSPAIGAAAMAFSSVSVVTNALRLRRFKPE"
     gene            13701..17411
                     /gene="nifJ"
                     /locus_tag="PK733_06700"
     CDS             13701..17411
                     /gene="nifJ"
                     /locus_tag="PK733_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOJ10267.1"
                     /translation="MGKKKTMDGNEAAAYASYAFTEVAAIYPITPSSPMAESVDEWSA
                     RGMKNIFGQTVKVVEMQSEAGAAGTMHGSLAAGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPGVFHVSARAIATHALSIFGDHQDVMACRQTGVAMLASANVQEAMDMGFITHLSAI
                     KSRIPFIHFFDGFRTSHEYQKINTTNYTDIAKLVDYDSIKNFRTRALNPQHPVVRGTA
                     QNPDIYFQGREAANSFYDRIPEIVEYYMKEYSSITGREYKLFDYYGAPDAEYVIVAMG
                     SVCDTTEEVVDYLNSRGEKTGLVKVHLYRPFSEKHFISALPATVKKIAVLDRTKEPGA
                     PGEPLYLDVVKVLSKFQSIYQCRKSINGNNGNNSNNSNNSNNGNSKSIYNIGDIDGIK
                     NSPIIIGGRYGLGSKDTRPSQILAVFNNLKTDESKDRFTIGIIDDVTGLSLAEEEIIN
                     TAPEGTINCKFWGLGSDGTVGANKSAIKIIGDNTDLYAQGYFSYDSKKSGGTTISHLR
                     FGKKPIKSSYLIYNADYIACHNKSFIYHYDVLKGLKKKGTFVLNCPWKPEELDEKLPV
                     SMKKYMVENDINFYIIDAIDIAEEIGLGNRINMIMQSAFFKLANVIPLDDAVRLLKES
                     IEKTYGKKGKEIVNMNNEAVNRGIAALDKIHIPVSWADIDEAVNKNPISRITTIKSEE
                     PDFVANIQIPIARHEGDELPVSAFNGMEDGSYPLGTTAYEKRGIAVFIPQWQMEKCIQ
                     CNRCSYVCPHATVRAFLLNKDEINNAPDTFKTLKAAGKSLEDYEYRIQISPLDCTGCG
                     NCADVCPAKGKALVMMPAEDEIENQTGNWEYAMTITEKKDLMELETLKGSQFARPLLE
                     FNGACPGCGETPYIKLLTQLYGERMMIANATGCSSIWAASAPSIAYTTNHEGKGPAWA
                     NSLFEDNAEYGYGMYLGVKQIRDRLEALIKEEIGDKEIGDSPAQIEHIAGDSPSLKSA
                     FLEWLENKEDGTGSKAASHKILDILSKYDYRGNKILEEIMDKKDFLVKPSVWIIGGDG
                     WAYDIGFGGLDHVLASGDDVNIFVMDTEVYSNTGGQSSKATPAAAVAKFAASGKRTGK
                     KDLGLIAMTYGHVYVAQIAMGADMNQTMKAIAEAERYKGPSLIIAYSPCINHGIKTGM
                     GTSIMEEKKAVETGYWQLYRFNPDLKKHGKNSFALDSKEPKESFIDFIKGEIRYSQLS
                     NTYPDRAGELFDIAEKNAVERYEIYRKLADTY"
     gene            complement(17493..18104)
                     /locus_tag="PK733_06705"
     CDS             complement(17493..18104)
                     /locus_tag="PK733_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ10268.1"
                     /translation="MVNRNISVRTVKMVQMAILAAIMLLMAFTPVGYLKTAGIEISFM
                     MIPVVVGAIVIGPGAGAVLGGVFGATSFIQCFGMSVFGAALLAINPFFTFIVCIVPRV
                     LMGWLAGLVFKGLSKMGKPKLVSFGVSSLSGALFNTVLFMTFLMLLFGKTEYIKGFQG
                     ELSLIPFVLAFVGINGLVEAIVCFVTGAAISRALIHYIPATEK"
     gene            complement(18186..18974)
                     /locus_tag="PK733_06710"
     CDS             complement(18186..18974)
                     /locus_tag="PK733_06710"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOJ10269.1"
                     /translation="MVFTADIGNSSITLAVFDNDGRLAFRSNLSTVKTRCEDEYIILM
                     LGIFEMYGFEQSAIDGAIISSVVPPLSNVIGGAVSKLFGCKPLFVGPGIKTGLDIKID
                     NHAQLGADLVANTVAASAVYTKPFIVIDMGTATTLTVVNSNSELCGVAILPGVQIALN
                     ALSGSTAELPYISLGAPKALLGKNTVDAMNSGVVYGNACMLDGLIDRLCEDFNIKSIS
                     TIATGDLANLIIPYCKHRFIYSPNLILEGLYLLYKMNQKKHKPM"
     gene            complement(19141..19308)
                     /locus_tag="PK733_06715"
     CDS             complement(19141..19308)
                     /locus_tag="PK733_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HOJ10270.1"
                     /translation="MLRESDNRHFRKIPVEKHDTAAWANIRTTKRVSKVTVPDEFQVE
                     NARDYVEENQK"
     gene            complement(19512..20375)
                     /locus_tag="PK733_06720"
     CDS             complement(19512..20375)
                     /locus_tag="PK733_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10271.1"
                     /translation="MSIIDLYKPDDNGNDNASPVNIYYKESYEQLELDNHFHNSYEII
                     YIVDGKVNFSIYGKEYNAISGNLIFINNFENHNLRVLTFPYKRYYILIDPTWFQSVIN
                     DPVILSIFKNRPPHFEHNITLDANMQGLVISLFNQILSEIENREAFWEILVKSYLQKF
                     FVLLYRNSSDVFPLRTYNSSMETVFNVQKYIEENCTEEINLKKLSELFYIDMYYLSRL
                     FKETTGFKFKEYLILQRISRAKDRLFYANDSITKVCMDSGFNNVNHFIRIFKKYTGTT
                     PHKYRLMNRST"
     gene            complement(20760..>21541)
                     /locus_tag="PK733_06725"
     CDS             complement(20760..>21541)
                     /locus_tag="PK733_06725"
                     /inference="COORDINATES: protein motif:HMM:NF017328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HOJ10272.1"
                     /translation="AEGTVFFKDHTTSLQECSYNQPLAILGSINNPNSLQENTNETKL
                     FMIDSEYIAGNLPRKGGNSVDLFGCAIFNGDKMIGELNGHETRLLLITRGEFKQGFFT
                     IEDPIMPELIIPIDIRQKESPDVKISFSKNRPAINVAIALEGDILAIQSGISYERPDM
                     NNIVENALNQRIKSGIEELIIKCQDLNVDVFKFGGKVARKFATIQNWEKYNWLERFNE
                     ADITVDVEFKIRRTGTMLKSSPIKISTIPDITNLGANMHLN"
BASE COUNT         7334 a         3504 c         4124 g         6579 t
ORIGIN      
        1 gtttgaaata ccagattaat gattcccaat gcgagatctg cttttgtagc caggtcactt
       61 atttttccaa agttagatgc tgtagtttca ggcggtagtg agccaaccgc tgtttcagtt
      121 gatatatctc ctatggtaag gtcaatattt gcgagattac ctccttgagc tgtttcaatc
      181 agatcatcaa agctccttac atttagcatc ctatttgaca atccaaggag tgtgcctcca
      241 ccaaccccgg taccacctat atgtcttacg cctgtattat ctgcagtaac cagtgctgtt
      301 ccggtaccca tactgactat tattgctttc tccagtttgc ttataaacaa gcctcccata
      361 cctatagccc taaactcatc aaccttgccg gtaggaatat tgaaaagatt atctgtaata
      421 aaagaggacc ctacaccagt gaccataatc ctctcaatat tgtccagctt taatttgttt
      481 atgcttaaga atttgccaaa cgctccatat acagaagaaa taggatcagc tgcttttact
      541 atcattggac taaacatttg tttgttatca aagcctatta tttttgtggt acttccacct
      601 atatctattc caattatttt tcccaaaaaa atactccttt tatatattta taatataata
      661 gcacctgaaa aggaaaatgt aaatactaaa aatagtaaat aaaatatatt tgttttattc
      721 aatacaagaa tggtattata ataatagcca aaatggagca tttttagcat gaaggagaag
      781 ataatgatga acgatacgcc aagaagcgac cgacttcata tagccttgtt tggaaggaga
      841 aataccggca agtcaagcct tataaatgct attacaggac aggaagcagc agtggtttca
      901 aatgttgcag gtactactac ggatcctgta tataaaagca tggaagtatt accaatagga
      961 cctgtagtgt ttatagatac acctggtata gatgatgctg gagaattggg aaggctgaga
     1021 actgagaaaa ccagagaagt tttaagaaaa acagatgtgg ctcttctggt aattggagcc
     1081 gatatgctat ggggcaagta tgaggttgag ttggctgaag aatttaggaa acggtcaata
     1141 ccttttataa ttgttgcaaa tcaggtagat aaaagtgcaa aacagagtga aatagagctt
     1201 gaaaattcca ctgttaccac tattgttaaa acttctgctt taacaggtga aggtattgat
     1261 aaattaatag aagaattagt aaaagtaaaa aaggaagatg accagcggct tcatttgatt
     1321 gatgatatta taaaagaagg cgatcttgca atattggtaa cacctattga taaaggggct
     1381 ccaaaaggaa ggcttatatt gccacagcag caggtattga gagatatact ggataataat
     1441 gctatggctt ttgttgtaca ggataacaga ttagaagaag caattaaccg gttgtcaagt
     1501 ccacctgcgt tggtaataac tgattctcag atatttggaa aggtgggaaa tgttgtacca
     1561 gaagggattc ctcttacctc tttttcaatt atatatgcaa gacaaaaagg tgatttaaag
     1621 aaattctatg aagcagtaaa agaaattgag aatttagagg atggagacct tattttaata
     1681 gcagaaggtt gtacccatca ccgtaaagat gatgatatag gtacggttaa aattccgtct
     1741 ttacttatga aaaagacagg caagcatctt aaatttaatc atgtttccgg cgggtttttt
     1801 gaagaagatt tatcaaaata taaaatggtt atccattgcg gagcatgcat gcttaataaa
     1861 aaggaaatgg agtacagaca ggaacactct caaagcagtg gagtacctat gattaactat
     1921 ggaattattc ttgcttattt aaatggaata cttgaaagag cattggagcc ttttaagtga
     1981 gacatgggga cggctctcct gtttccctgt tcagataaca gacagataag attcttctgt
     2041 ctctagtgta gaaagttgca tagtaaaagt actttttaca tattaacaat attttatatt
     2101 acgctataat taaaagcata gacattatta tttgtatgag tgttaatgat ggtaattctc
     2161 tggaatgtgt acaccaaaac atattacagg agtgtattcc tatgagaagt ttaaagagaa
     2221 acaccagtat attgcatatg tatttcacat cttatatagc gatatttgcg atacctttag
     2281 ttatattagg gattgtatta tatagtcatg ccataataga acttcagaat gaggtagaaa
     2341 ggtacactca ggataaagta ataagaatga agaacactct ggatgctaga ttgcttgaaa
     2401 ccaaaaaaac cgctctaaaa atatcttctg atgaaaatat caaaccacat atggtaaatt
     2461 taaacgatta ccgcagcaaa aatgccattg atcaattaaa tcgatattgg gataaaaact
     2521 taattgataa cctttttgtt tgctttagaa atgaaaaata tttatatgga gtagccggga
     2581 aaacctcctt tgatactttt tttgcaaata ctatcggtat agactataat acaagcataa
     2641 gcttcgctaa tgcacttcaa aaaactgata aatcaatgtt tttaggtata agcggaatac
     2701 cttcaggttc atcaaaaaat atagacaact taatttgcct gtatcctttg ccattaggag
     2761 gagtcagcag ttatggtgta ctaggaataa taataaacca gggtactatt gagaatcttg
     2821 caggtgatat acttggaaac tcaaaaggat ctgtatttat atataatgaa gatatgcaaa
     2881 gaatatttac aattggagac aaagattttt tcccgtcaga aagagttatt gaaattacag
     2941 gaggaaatta ttcagcagga gtaagcaaat caaaattcga gggtaggaac tattcagtaa
     3001 taaaaacagt ttctgaagaa tccgggtttc attttatcgt aataatgtcc cctgaaagct
     3061 atctcaacag agtaattatc atgagaaagc aggtaatggt tataatattc ggggtaattt
     3121 taacagggat actacttgct tatgtttttt ccagaaaaaa ttacaagcct ataaaggtat
     3181 tgtttgaaaa aatgaacaat tatacttcac taagtgaaca aaaggaaatt aatgaacctt
     3241 ctattattaa caaaaaggtt aacgaaatag aaggtatcga aaaaatgatt aaaaatattg
     3301 tagacaaaaa ctatggacta actgagcagt taaatcatca tcagtcattt gtaagagatc
     3361 agattttact tatgctgtta aaaggtcagc ttaatgagaa agaaaatata aaagatttat
     3421 tagctttata taatatgaaa ttcgactatt tatattcgta tgtatcagtt atatccatac
     3481 agaaagttaa ggaatatgag acgactgcag ctgagttatc tctttatatt aaaggggtaa
     3541 tagatagtaa attgaaaaac aagcaattct attggataga cacggttcag ggtgattttg
     3601 ttctattggt taacacaacc tatgaaaatt gctccaaaat catgaaggta aatctcttaa
     3661 aagatattca gatatttttg caaaataacc tgggacttaa aattaatatt ggtgttggaa
     3721 gagtgtatga taatatatcc cagattagta tatcttttat ggaggcattg agtactttgg
     3781 attacaaatt attgataggc tatggtaata ttattttctt tgatgaaatg gtatcttcgg
     3841 atacaatggt ttattggtat cctgtagaag agcagattcg cctgtcgcag tgtataaaac
     3901 agggaaatat ttatatattc agagatataa ttgatgatat tatcgacagt attaaggaaa
     3961 aacatcttcc cataggtatt accagaacta tatgttttgg aatgattaat actataatag
     4021 aggttcttaa tgagtttaat ataaaggatt ttgatagtga tgtagaggaa cttacaacct
     4081 ttacatcatt ggatgacctt aaaaataaga tggaaaatgt ggtatataaa atatgtaaat
     4141 atataagcga aaagaaggaa agcaataatg agacattaaa aatagcaata attgaatata
     4201 tacacgagaa ttacaccaat aatatgttta atttggatat ggtggccgat aaatttaaca
     4261 tttcttcaaa atatttaagc aggttcttta aacagcaaat aggatttaac tttgtcgatt
     4321 acatgaaaga attaagaata aattattcaa agaagttgct aagtgaaaca aaccttttaa
     4381 taaaggatat tgttgagcag gtaggataca gtgatgttgc cagctttacc aggactttca
     4441 ggcaaatgga aggtattact cccggacgtt ttcgggagat ggcgttaaat acagtaacac
     4501 aacaagaaaa aaatagtttc taaattatca tcaagggaaa tagttaatgt attttagtaa
     4561 aaaagctata atattcgttt aatatagaga aatgcatagc tgaaatacct ttagcatatt
     4621 ttcaaaacta ataataattc atataatcat aattaagaaa tgatagttat acacttactt
     4681 aatagttatt tatggaggtc gccttatgaa atcggaagct ataccggaag ttgctattag
     4741 tgttaagaaa tccggcagca gtacacttaa aaaaatgtta agtacttatg aattatattt
     4801 atttattctt cctactgtta tttactatat tatattccat tatgcgcctc tttatggtat
     4861 tcaactggca tttaaagatt ttattgcaac tgaaggaatt tggggaagtc cttgggttgg
     4921 cttaaaacat tttaagctat tcttcagttc acattatttt tgggaattat taagaaatac
     4981 agttggaata tctctatatg ctttagtagc aggctttccg atgcctatta tttttgccct
     5041 tatgctaaat gagattagca gcaaacgtta taaaaaaatt gtacaaactg tagcgtatgc
     5101 tcctcatttt atttcaaccg ttgtcatggt aggaatgttg tatatatttc tacaccctgt
     5161 atatgggata gctaattctc taataaaagt ttttggagtg caagctgttg attttctaaa
     5221 cagaccggaa tactttaaaa gtatttatgt atggtccgga atatggcaga gtacaggatg
     5281 gaattcagta atatattttg ctgcattatc aagtttggat ccagagcagc atgaagcagc
     5341 aataattgac ggtgccacca gattacaacg tatttggtat ataaatatac cagtaattat
     5401 tcctactgct gttattctgt tgatactcag tgcaggggga attatgagtg tcggttttga
     5461 aaaaatttac ttgatgcaaa caccactaaa cgcaaataca tctgaagtaa tatcgacata
     5521 tgtatataaa gtaggattac agggtgcacg ttttagtttc acaacagcta taggtttttt
     5581 tgattcaatt attaacttca tactacttat aattgtaaat cgagtggcaa aaagcctttc
     5641 atcgacaagc ttgtggtaag ggggtaacaa gtaaatgaaa aaaagcaaag atgatataat
     5701 ttttgaaacc ataaacacta tattaataac aatatttttt gtaatagtac tttatccgct
     5761 gctatttgta ttaagcgcat ctataagtga tccatcactg gtttctcagg ggaaaattat
     5821 attgcttccc aaaggcatca attttaacgg gtataagaga gtattagcat attctgatat
     5881 atggttaggt tatagaaata ccatatttta tgcaattaca ggtactttga taagcttgtt
     5941 cctcactcta acctccgctt tttctctctc aagaaaagat tttataggga agaacatttt
     6001 tgtagtattc tttacagtaa caatgttctt ttcaggaggg ttaataccaa cttatatggt
     6061 aataaagagt ttgggaatga gaaatactgt ttttgcacag ttgttacccg gtgctgtaag
     6121 cttcatgaat ataattgtag taaggacatt ttaccagacg agcatccctg atagtttgca
     6181 ggaagccgct tcaattgatg gctgttcaat attcaggttg tttgtttcaa taatactgcc
     6241 cctatcaaaa ccaataattg cagttatggg gttgttttac ggtgttgccc aatggaacag
     6301 ctactttaaa gcactgattt atatttctga tagaaaaatt ttcccgctgc agttaatttt
     6361 aagggaaatt cttgtggtta accaaattaa tatggactta ctcatgaatg aaggtgagtt
     6421 agaagttatc cataaaagag ttgagcttgc aaccttatta aagtattcag ttattgttgt
     6481 atctactttg ccggtaattg cagcatatcc attcctacaa aaatatttta taaaaggagt
     6541 tatggttggg gcaataaaag gctagagtgc gattaaggca tttgtaaatg tccataaagt
     6601 tttatagttt accattaaca atttaaataa tatgcacttt gatgattata cgttccatat
     6661 gttgacagtt catataattt ataatggtaa cttaaaaaat aaaaaataat aaaatcaagg
     6721 gggaaaagca atgttaaaaa agccatttgc taaaatctta agtttaattg ttgtaatgct
     6781 tgttttagtt tcgtccttta caggatgtgt aaaaagctcc ggaacagatc tttcatcaga
     6841 acaaacaaag aagccggata ccactgaaaa acaaaaacct acagaaccag caccaaatgc
     6901 tcttgtatcg gacaaaaaaa taactttaaa agttatgtcc caaagagatg ctatgggtgg
     6961 tccatgggaa gatttgaggc tgtttaaaga tcttgaagaa atgacaaatg ttcattttga
     7021 gtttgatgtt gtatttgaag gctttcagga gaaaaagaat ctcgcactgg caacaggaga
     7081 tctcccggac attttccttg gtgcccttaa tgttgatgat gaagagaatt atggtccgca
     7141 aggcatgtta attgatctta ctgaactaat tgacaaacat ggtaaaaaca tgaaagcaac
     7201 attcagcaaa taccctacac tgtttaaaac catgactgca agtgatggta aaatatatgc
     7261 ccttggacag atacttgtta caactacaat ggggtcatat aaaccggtaa ttaactctac
     7321 ctggttaaaa aaccttaatt taaaggttcc tgaaacaaca actgatctct ataatgtact
     7381 taaagagttt aaaacaaagg atccaaatca gaatggcaag agtgatgaaa tacctctagg
     7441 agatattgga tttaatcatc ttaacgggat gctccttact gcttttggta caatggcaca
     7501 ggatatatca gttaaagacg gtaaagttgt atttacacct atgacggata gctttaagca
     7561 ctacttggaa tatattaata agttgtataa agaagaactt attgacccta tgtttatttc
     7621 tcacagtatg gatgaattcc tggcaaaaac aaaacaaaat ctttatggtg tactaggaaa
     7681 cataaggcaa tcagattgga cgcaatttca aatattgaat cctttaacat ctgaatataa
     7741 tagtgaaaaa tttagcagct caacagattc attgtattct gccggaagat ttgcaataac
     7801 taagaataat aaatacccgg ttgaatcaat aaaatgggct gatctgttct tcacagaatt
     7861 ggataaaggt gttaatggag taaccggcgc agctctgtgg attggtcgca gaggtgtgga
     7921 ttgggatttc actgatgata agaaagccgt taaatactta ttcacacctg atgaaggtac
     7981 aacagaaatc caaactttac tgaaaaaagt ttcacccagc caaactgggt ttgtaggcga
     8041 actaattatg aatgtgccat tcgagtctcc atatttgaaa tgggttgggg aaaacaataa
     8101 aaataatgta ttcccatatc tgagaaatga aaggttattc ccggcaggag ctcgtttcac
     8161 caaggaagag caagaaaaga taagtatact aaaaactgat attactaaat ttgttgagca
     8221 ggaaagagcc aaatttgcta caggagacaa ctcctttgac aactggaata attacattaa
     8281 gtctttagaa agtatgaagg ttgacgagta tattaaactt catcaacaag catatgatag
     8341 gtttatgaag ggcggaaagt aatattgtat gcttcctggg aaaagcatct gttgcatgca
     8401 acagatgctt ttcccattct tgaggataca tcttcttttt ctgtagactt aaacgctaca
     8461 atccgttgct gaagtgaata ttatatatgg aaatcgtttc ttgacatatt ttccctcaaa
     8521 tatagaaaat gcaacccttt tttcatatcc ccttgacacg ttatttattt acatttaaaa
     8581 tgttaatgat aataattctc aataaaatgg caggggggag taaaaagtga ggcttaatgt
     8641 aagcacaagc gctatattat atgcatttgg gttaacaaca ttggcaggaa tatctacagg
     8701 cataggaggc ttgtttacaa taatatgcaa aaagaccaac aaaaagtttc tatcggtcat
     8761 acttggattt tctgcaggag ttatgattta cttatcgttc atggaaattc ttcacgaagc
     8821 aatgaattat ttaactaatg tgcttggtga aacaggcggt tcatgggcag ctatagcagc
     8881 tttctttgga ggagtttttc ttattgctat catagataaa cttatacctt caaaggataa
     8941 tcctcatgaa agccataaaa tagaggaaaa gcatacctgc gattcaagtt ataacaactg
     9001 gaagctttta agaacaggtc tgcttacagc agttgcaata acaatacaca attttccaga
     9061 gggtattgct tcttttattt ccggacttaa agaaccatca ctgggaatag caatagcaat
     9121 tgcaatagca atacataata tccctgaagg aatagcagta tcaataccta tttactgtgc
     9181 aacaggcagc aaaagaaagg cttttttgct ttctatggct tcaggcttgg cggaaccttt
     9241 aggagcatta gtagggtatt taatacttca acctttcctt aatgacctgg tttttggtat
     9301 tttatttgca gctgtagccg gtataatggt attcatatca tttgacgaac tgctgcctac
     9361 agcagaggaa tatggtgaac accatttatc tatcctggga cttatagggg gcatggctct
     9421 tatggctgta agcctcatat tattcattaa atagtctttc gaacaaattg taaattcagt
     9481 gcaaatgctg gttcagctgg taaatgatgg cataagattt aactaaggtt taccgctgaa
     9541 ttagtttttg cactaaaata gactatgggt gcatatttta tttttgaaga aattattgga
     9601 agaagcatta gtgtaggctc taagtaccgt aacgatagct tctggaatat atttcttcaa
     9661 aaatattaaa tgcaccaata gactatatat ttgtaacaca tatttcccta tatgaatatg
     9721 atattataga ttatttcaat tatttaatta cttaatcact tacttattac catgagatat
     9781 ccaatacatt aattgcaatg cttaatgaat gtattggcag tcacaaacat ttttgctgtg
     9841 taatcttaaa attgctcagc aaattgttcg acatgaaaaa tggatcatat gaaaggggga
     9901 gtatggatga ccgagaacag aggaggattt ggctgcgcag ggtttggcaa tgaatggata
     9961 tggtggataa ttataattat aattgtcatt tgcctgtttt gtccgggaat ttttggcggt
    10021 ttcggatgca gagaataaca tataattata ctgctaatta agctgcaggt attaattatt
    10081 atggagcatg catagctaaa taatgagtta tatgtccaat gcacttaatg atatggtatt
    10141 gggcatataa tttttttttg caatttactt cctgagtata aacaaatatt tatctgttag
    10201 actttgtaat actgtggtaa ataataataa aattattatg aatactacta caaatgcgct
    10261 ttccattggt aacataacaa agtagtatta atatattcgg cttttactat ttaaaaaact
    10321 tataatagca aacaatagta taaatataaa tataagacaa gatggtgata gtaatgagaa
    10381 agcaaagact tataataaat ggaatgacat gtacagcttg tgcaaaggct gttgagagaa
    10441 gtgtaggaaa gctggaagga gtaaataaag ctattgttaa ttttgcaact gagaaactcg
    10501 atatagaatt tgatgagaat aaagttggta taaataacat actggagaca gtaaaaaagg
    10561 caggttatag agctgaggaa gaagcagaag aaagtataaa agccagggtt gtatatgaca
    10621 cttcaaaaat aaaaacttca cagattaaag gtgcagtatc aaaagcaggg tataaggctc
    10681 ttgaaattga aagggtagaa aagaccgatg cagatacgga tagaaaggaa agggagagaa
    10741 agcttctttg gacaaggttt atagtagctt tatccttttc aataccttta gtttatattg
    10801 ctatgggttc tatgcttggg cttcctgttc ctcatattat ttcgccggag aaataccccg
    10861 taaactttgc aatcatgcaa ttagcattaa cactcccaat agcagccgcc gggtacaggt
    10921 tttacacagt aggtataaaa caacttttaa agggacatcc caatatggat tccttaattg
    10981 caattggtac aggcgctgcg attatttatg gtttatatgc tatagccaga atactggaag
    11041 gagaactact ctatgtacac aacctgtact ttgaaacagc aggtatgata ataaccctgg
    11101 ttatgctggg aaaatacctt gaaacagtag ctaaagggaa aacatcagag gctatcaaac
    11161 gtcttatggg tctgacccct aaaactgcaa ttattattca tgatggaaga gaaatagtaa
    11221 tacctgtaga ggaagttgaa acaggagata taataatagt aaaacctggg gagaagatcc
    11281 ctgttgatgg tgaagtggtt gaaggacaca catcagtaga tgaatccatg ttgacaggag
    11341 agagtattcc tgtagaaaag actgttggag acaaagttgt cggtgcaact ataaataaaa
    11401 acggtatgat aaagttcaga gccactaaag tagggaaaga tactgtattg gcacaaatca
    11461 tcaaattggt ggaggatgcc cagagttcca gagctccaat tgcaaaaatg gccgatgtta
    11521 tatccggtta ctttgttcct gttgtaataa gtactgcatt gcttgcaagt attttatggt
    11581 acatttcagg tcagcccccg gtatttgcgc tgacaatttt catatctgtt ctggttattg
    11641 cctgtccttg cgccttaggt cttgctacac ctactgcaat aatggttggc acaggaaaag
    11701 gagcagaata tggagtactt gtaaaaagcg gagaagctct agaggcttcc cataaaattc
    11761 atacggttgt gctggataaa acaggtacca ttactgaggg caaacctaca gtaacagata
    11821 ttattgtact tggagatatt acccaaaccg atcttttgca gttggcagca tcttcagaaa
    11881 taagttctga acatcctttg ggcgaagcta ttgttaatat ggcaaaaaag gaaggacttg
    11941 atttcttgaa taccgacagc tttgaggcta ttccaggtca tggaataaag gctgaaattc
    12001 aaggaaagct ggttttacta ggcaataaca aattaatgga tgatagaggt attgatttta
    12061 atgtaaaaat agcagaaaca gataccagac taggacagga agggttggga aggcttgcgc
    12121 aggaagagtt tgataggctt gcacaggaag ggaaaactcc aatgtttgtt gcttttgata
    12181 ataagccggc aggaattata gccgttgccg atgttgtaaa agagagcagc aaacccgcta
    12241 tagaggctct tcacaaaatg ggaattgatg tagtcatgat tacaggagat aataaaagaa
    12301 cagcacaggc tatagcaaga cagataggaa tagatagagt attggcagaa gtactgcctg
    12361 aagataaggc aaacgaggtg aaaaagcttc agcaggaagg gaaaaaagta gcaatggtag
    12421 gggatggcat aaatgatgca cctgctctag ctcaggctga tgttggtatt gcaataggtt
    12481 caggtactga tgtggctatg gaatcggcag atatagtact tatgcgaagt gaccttatgg
    12541 atgtgtctgt ggcaattcag cttgctaaaa agacaatgag aaacataaag cagaacctgt
    12601 tctgggcttt tttatataat tctgcaggaa tacctattgc ggcaggactg ctttattatt
    12661 ttggaggtac gttgctaagt cctgcaatag gtgcggcagc catggcattc agctcggtat
    12721 ctgtagtaac taatgctttg agattgagaa ggttcaaacc agaatagatg ctgtgactct
    12781 tgaagaaaaa gatcgtgagg agatatcaag acttcgtgta ctaaagaaca tgaggaagga
    12841 ggctcctaag cctcctttat tgaactgctt ttttctatat tttattaaag tatgccctaa
    12901 tctctgaaaa ctccctctcc acatcttcca caccagaaga gaagcaggaa gaggataata
    12961 aaccagagaa ttgtgtcatt ttcaagtata ccacctaaaa aacctctgtc tgccatattc
    13021 aatagtctcc tttctatttt ttattccctt gctttaactt tctagttgat attaattctg
    13081 cttaaagagt acagagtatt ttatattact catctctaaa aacaaaacca tcaccgcatc
    13141 cgaaacacag tatcaagaaa acgagaatga agaacaatat ttcactgcgg cctccgaaaa
    13201 ttcctttaac tggacctccc attataaaac ctcctttcat ggaattcctt gaatgtagat
    13261 tgcaaaatgt catgggtgaa ctttaaattt actttaaaga agttttttcg ctgctaattt
    13321 atactattca aaacaaggag atatggttac aaaaatcttt taaagctttt tcatacatat
    13381 tggagataca ttggagatac atattgggga cgttccttca cttattgttg gggacgttcc
    13441 ttcacaattt ataaattgtc actgctgcat taactcagat ttcggtgcaa aatgcaagat
    13501 gaagtgttac aaattacaat gtattgaccg ctgaatgagt ttttgcaccg aaatctaaat
    13561 tttgttcact tataatataa caataatatg gaataataaa tttgttgatt tattggggac
    13621 gtaggggacg tgatttatgt atggggggac gttccttcag atttataaaa catatactac
    13681 ctacctgaag gaggaaagtc atgggaaaaa agaaaactat ggatggaaat gaggctgctg
    13741 catatgcatc atatgctttt actgaagtag ccgccatata tcctattacg ccttcatcac
    13801 caatggctga aagtgtggat gaatggtcag cccggggaat gaagaacata ttcggacaaa
    13861 ctgtaaaagt ggtagaaatg cagtctgaag cgggggctgc agggacaatg catggctcac
    13921 tggctgcggg tgctttaacc acaacttata ctgcatctca gggactgcta ttaatgatac
    13981 ccaatatgta caaaatagct ggggaattgt tgcctggcgt attccacgta agcgccaggg
    14041 ctattgcaac ccacgctctg tcaattttcg gagatcacca ggatgttatg gcatgccgtc
    14101 aaaccggtgt tgcaatgctt gcatctgcaa atgtgcagga agcgatggac atgggcttta
    14161 ttactcatct gtcagcaata aaatccagaa ttccatttat tcactttttt gatggtttcc
    14221 gtacatctca cgaatatcaa aagattaaca caacaaatta tacagatatt gccaaacttg
    14281 tagattatga ttccataaaa aatttcagaa caagggctct taatcctcaa catcccgtgg
    14341 taaggggcac tgcacaaaat cctgacatat attttcaggg aagggaagca gcaaacagct
    14401 tttatgacag aatccccgaa attgttgagt attacatgaa agaatacagt tccattacag
    14461 gaagagaata taagctgttt gattattatg gagctcccga tgcagaatac gtaattgttg
    14521 ccatgggttc agtttgtgat actacagaag aggtggttga ctatttaaac agccgtggtg
    14581 aaaaaacggg tttggttaaa gttcatcttt atagaccatt ctctgagaaa catttcatat
    14641 cagccctgcc tgctactgtt aagaagatag ctgtactgga cagaacaaaa gagccgggtg
    14701 caccgggaga accgctttat cttgatgtgg ttaaagtatt atcaaagttt caaagcattt
    14761 accagtgtag aaaaagtatc aatggtaata atggtaataa tagtaataat agtaataata
    14821 gtaataatgg caatagcaaa agtatttata atattggcga tatagatggc attaagaatt
    14881 cacctataat tattggcgga agatatggcc ttggctcaaa agatacaaga ccttcccaaa
    14941 tacttgcagt atttaataat ttaaaaacag atgaatccaa ggaccgcttt acaataggca
    15001 taattgacga tgttaccgga ttgtccctgg ctgaggaaga aataattaat actgcaccgg
    15061 aggggacaat aaactgtaaa ttttggggct tgggttctga tggtacagta ggagcaaaca
    15121 aaagtgccat taagataata ggtgataata ctgatcttta tgctcagggc tatttttcct
    15181 atgacagcaa gaaatccggc gggactacca tatcccacct acgttttggc aaaaagccca
    15241 taaaatcttc atatctcata tataatgcag attatatagc atgtcataac aaatctttta
    15301 tttatcatta tgatgtgctt aaaggcttaa agaaaaaagg gacttttgta ctgaattgcc
    15361 cctggaaacc tgaagaatta gacgagaagc ttcctgtttc aatgaaaaaa tatatggtag
    15421 aaaatgatat taacttttat ataattgatg ctatagatat agctgaagaa ataggacttg
    15481 gtaacaggat taatatgata atgcagtcag cattttttaa gcttgccaat gtaatccctc
    15541 tggatgatgc tgtaaggctt ctcaaggaat ctattgagaa aacctatggc aaaaagggga
    15601 aagaaattgt gaacatgaac aatgaagctg taaatagagg aatagcagca ctggataaaa
    15661 tccatattcc tgtatcatgg gcagatatag atgaagcagt aaacaagaac ccgatcagta
    15721 gaattacaac catcaaaagt gaagagcccg attttgtagc taatattcaa atacccattg
    15781 cacggcatga aggggatgag cttcctgtaa gtgctttcaa tggtatggaa gatggttcat
    15841 acccgctagg cactactgct tatgaaaaac gtggtatagc agtatttata ccccagtggc
    15901 agatggaaaa atgcatacag tgcaaccgtt gttcttatgt ttgcccccat gctactgtaa
    15961 gggcatttct gttaaataaa gatgagataa acaatgcacc tgatacattt aaaacactga
    16021 aagcagccgg caaaagcctg gaggactatg aataccgtat ccagatttct ccccttgatt
    16081 gtacagggtg tggtaattgt gccgatgttt gcccggctaa agggaaagca ctagtaatga
    16141 tgcctgctga agatgaaatc gaaaatcaga cgggaaattg ggaatatgca atgactatta
    16201 cagaaaagaa ggatcttatg gaactggaaa cccttaaagg cagtcaattt gcgagacctc
    16261 ttcttgagtt taacggagca tgtccaggct gcggagaaac accatatata aaactcctga
    16321 cacagttata tggtgaaaga atgatgatag caaacgctac cgggtgttct tccatatggg
    16381 cagccagtgc accatctatt gcctatacaa ccaaccatga aggaaaagga cccgcctggg
    16441 caaattcgtt atttgaagac aatgccgaat atggctatgg catgtacctg ggtgtaaaac
    16501 aaataaggga taggctggaa gctttaataa aagaggaaat tggggacaaa gaaattgggg
    16561 acagtcctgc acaaattgag cacattgcgg gggacagtcc ttcacttaaa agtgcatttc
    16621 tagaatggct tgagaacaaa gaggatggca cgggttccaa agctgcatca cataaaatac
    16681 ttgatattct tagtaaatat gattatagag gaaataaaat cctggaagaa atcatggata
    16741 aaaaagattt tctggttaaa ccatcagttt ggattatagg tggagacggc tgggcttatg
    16801 atattgggtt tggaggactg gatcatgtac ttgcctctgg agatgatgtt aacatttttg
    16861 taatggatac ggaagtgtat tcaaatacag gtggacagtc ttcaaaagca acaccggctg
    16921 cggctgtagc aaaatttgct gcttcaggta aaagaacagg taaaaaagac ctgggcctta
    16981 tagcaatgac ctatggccat gtatatgtag ctcaaatagc aatgggtgct gatatgaatc
    17041 aaacaatgaa agcaattgcc gaagcggaaa gatataaggg accgtctctg attatagcat
    17101 actccccatg tataaaccat ggtataaaaa caggaatggg tacaagcatt atggaagaga
    17161 aaaaagctgt tgaaacaggg tattggcagc tatacagatt taatcctgat cttaaaaaac
    17221 atggaaagaa ctcttttgca cttgattcca aggagcctaa ggaatcattt atagatttta
    17281 taaaaggtga aatacgttat tcacagttat ccaatactta tccggataga gcaggagaat
    17341 tgtttgatat agccgagaaa aatgctgtgg aaagatatga gatttacagg aagctggcag
    17401 atacctatta ggtattgatt gaataataac cctaaattcc tgcaattaat atctagaaaa
    17461 gcaggaggcg taccccctgc tttttcaacc ttttattttt cagttgcagg tatataatgt
    17521 ataagtgccc ggcttatagc agctcccgtt acaaagcata cgatggcttc aaccaaacca
    17581 tttattccga cgaatgccaa tacaaatgga attaggctca gctctccctg aaaacctttg
    17641 atatactctg ttttgccaaa taaaagcatc agaaatgtca tgaataaaac tgtattgaat
    17701 aatgctccgg ataggcttga cactccaaaa gacacgagtt tgggtttgcc cattttactc
    17761 agccctttga aaacaagccc tgcaagccat cccattagaa ctctgggaac aatgcacaca
    17821 atgaaagtaa agaatgggtt gattgcaagc aatgcagcac caaacacact cattccaaaa
    17881 cactggataa agcttgttgc accaaaaacg cctccaagca ctgccccggc ccccggacca
    17941 atgactattg cgcccaccac cacaggaatc atcataaagc tgatttcaat acctgcagtt
    18001 ttgaggtagc cgacaggtgt gaatgccata agcaacataa ttgcagcaag tatggccatc
    18061 tgtaccatct tgactgtacg tactgatatg tttctgttta ccataataaa aactcctttg
    18121 ctattgcccc ttacttattt cagggtgcaa tgcctattta tttataaaag cgtttatacc
    18181 cgcttttaca tcggtttatg tttcttctga ttcatcttgt aaagcagata caagccttca
    18241 agaatcaggt tgggtgaata tatgaatcta tgtttgcagt acggtataat taggttggct
    18301 aaatctcctg tagcgatagt gcttatagat tttatattaa aatcttcgca taagcggtcg
    18361 attaaaccgt caagcataca ggcattgcca tatacaacgc cggagttcat ggcatcaacg
    18421 gtattcttcc ctaacaatgc tttaggtgct cccaatgaaa tatagggcag ttccgcagtg
    18481 cttcctgaca atgcattaag tgctatttgg acaccgggaa gaatagcaac accacataat
    18541 tcactatttg agttaacaac agtaagtgtt gttgcagtgc ccatatcaat aacaataaag
    18601 ggctttgtat atacagcaga tgcagcaaca gtgttagcta ccaaatcggc accaagttga
    18661 gcatggttgt caatcttaat atcgaggccg gttttgatac cgggtcctac aaacaagggc
    18721 ttacagccaa acagctttga aacagcaccg ccaataacat ttgataatgg cggaactaca
    18781 gacgaaataa tcgcaccgtc gattgcactt tgttcaaagc catacatttc aaaaatgcca
    18841 agcattaaga taatatattc gtcctcacat cttgttttga ctgtggacag attcgagcgg
    18901 aaagcaagtc tgccatcatt gtcaaagaca gccagtgtta tactggaatt gcctatatcg
    18961 gcagtgaaca ccatttatat acacccctat aatattatat tagcaattat tttttatgtc
    19021 aaacaaattc gagcatatca atccccacac gggtgcttat tttattttta aaaaatgcac
    19081 cccgggcgta taggtgcatt ttcttcctat gaaataaaat atggcggcat catgcctaca
    19141 ttacttttgg ttttcttcca cataatccct ggcattttca acctgaaatt catcaggaac
    19201 tgtcactttt gaaacacgtt tggtcgttct tatatttgcc catgcagcag tatcatgctt
    19261 ttcaacaggt atttttctaa agtgtctgtt atcagattct cttaacaaaa taattatctc
    19321 cttacatatt ttttgttggt atatttttat tgttcccaaa attaattttt caagtagtgc
    19381 tatatcaaaa aatacttcaa atcgacaaaa tacttccaca aaatatttcc agtgtatctt
    19441 tcagtataca tttcaacata cctattatta gatgaatatg tttcttcaaa aattgtaaat
    19501 gcaccttatt attaagtact tctgttcata aggcgatatt tatgtggtgt agtaccggta
    19561 tatttcttaa aaatcctgat aaaatgattc acattattaa aacctgaatc catacagact
    19621 tttgttatac tgtcatttgc ataaaaaagg cgatcttttg ctctggaaat tctttgaagg
    19681 atcagatact ccttgaattt aaaaccggta gtctctttaa ataaacggga taaatagtac
    19741 atatctatat aaaaaagctc tgaaagcttc ttcaaattta tctcttcagt acaattttcc
    19801 tcaatatact tttggacatt gaaaactgtt tccatagagc tgttatatgt tctaagaggg
    19861 aaaacatcac ttgaattcct gtacaataat acaaaaaact tctgaagata tgactttact
    19921 aatatttccc aaaaggcttc cctgttttca atttctgaaa gtatttgatt aaaaagagag
    19981 ataaccaatc cttgcatatt tgcatcaaga gttatattat gctcgaaatg cggaggacga
    20041 tttttgaaaa tagatagaat aacgggatca ttaataactg actgaaacca ggttgggtct
    20101 attaatatat aatatctttt ataaggaaag gtaagaactc taaggttgtg attttcaaag
    20161 ttatttataa atataagatt acctgagata gcattatatt cttttccata tatactaaaa
    20221 ttaaccttgc catcaactat ataaataatt tcataggaat tgtggaaatg attatccaac
    20281 tccagctgtt catatgattc tttataatag atatttacag gacttgcatt atcattgccg
    20341 ttatcatccg gtttatataa atcaattatt gacactttaa aataaacacc tcacagcttt
    20401 tcaaaattta acaataccca aaatccagga tacaagacat gatatagatt ttacaactca
    20461 attcttaatt ttctcaatat ttcaaattat aatttcaata ttgagttata gccatactat
    20521 atcatatgta aatacatagc agcaaccaaa taagcctgtg tgggcgagat agaggcaaat
    20581 tatctaaaaa cataatacac tacagtgcct aaacacaata aattcaataa taccgtccca
    20641 aaagcaatac gaataaactt tttacccaaa caatacttaa agaaggataa ttcatatata
    20701 aatacaagaa ctgctgcaat aattaaagcc gctttcattt gtgttcacct tccgatttct
    20761 taatttaggt gcatatttgc acctaaattg gtaatatctg gtatagtact gatttttatt
    20821 ggagaactct ttagcattgt acctgtcctc cgaattttaa attccacatc aactgttata
    20881 tcagcttcat taaaacgctc tagccaatta tacttttccc agttttgaat tgtagcgaat
    20941 ttccttgcaa ctttaccgcc aaatttaaaa acatccacat tcaaatcctg acacttaatg
    21001 attaattctt caatacctga ttttattcgc tgatttaaag cattttctac tatattattc
    21061 atatccggtc tttcatagct tattccactt tgtattgcta aaatatcacc ttcaagggca
    21121 attgcaacat ttattgcagg tctgttttta ctaaaactga tttttacatc agggctttct
    21181 ttttgtctta tatcaatcgg gataataagt tcaggcataa taggatcctc tatagtaaaa
    21241 aaaccttgct taaattctcc tcttgtaata agtaaaaggc gtgtttcatg tccatttaat
    21301 tcacctatca ttttatctcc gttaaaaatt gcacacccaa atagatcaac tgaatttcca
    21361 cctttgcggg ggaggttacc tgctatatat tcactgtcaa tcataaatag tttggtctca
    21421 ttggtatttt cttgtaaact gttgggatta tttatggagc cgagtatggc taaaggttgg
    21481 ttatatgagc attcctgtaa tgaagttgta tgatctttga aaaaaacagt accctctgct
    21541 a
//
LOCUS       DAOSDD010000053        21533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1061_length_21533_cov_5.448180, whole genome shotgun sequence.
ACCESSION   DAOSDD010000053 DAOSDD010000000
VERSION     DAOSDD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21533
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1061_length_21533_cov_5.448180"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PK733_06730"
     CDS             complement(<1..575)
                     /locus_tag="PK733_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10273.1"
                     /translation="MDWSRAKNIFIIIFITLNLFLLIYSGIYKIQGSIPKETATSILS
                     ILQKKGIRIDENCQIPGYNKKTPMLELGNGNINKEKVVSVLLGKDFASMISFNDNDQD
                     KTSKIDKADEDDIYETGEKTLVFTGSGSFIYRDANPSALGTNDENDDKKSFSIKNDNI
                     SEKEKKQIEKDSKELIESLGINASSFFLDRYV"
     gene            complement(578..2053)
                     /locus_tag="PK733_06735"
     CDS             complement(578..2053)
                     /locus_tag="PK733_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10274.1"
                     /translation="MLSFNREKVKSAVLIVLVLSSIIQVGILWDYQSHRFPFNFISKV
                     FSSQSNNDKTDIVEIARKDIFSPYRITISNGNRTHWVIGKDDYIYNELWDEACGYLEK
                     VLTSGEGHSVNMNTWIDLTVKKSIMIEFKTGIKTGLLKWFLDITGSSSDYFDSIQKIF
                     ILPYEDINNNNTVYIYSSKGLYRYIVPFNKKGMLKSGYTKTINNLEYNGNIVQYNTFK
                     EIDPNNVISKKLQLSISPDILCVAEGIKYYKYPSAIYYDNRKTADIEEIARVILGNEK
                     ESFDRYIENNAVVFKNINNTYRLYNDGLLEYKYIPGVDDQEKSDIGSAFEKAYIFISR
                     IKKGYIDPETDLYLKQVKDENQGYYEFIFDYKLGDLPVYINFQDKSGDKEPVKSAVSI
                     KANGKRIIECRWYLASIEKSRTQSEYNIYFEDMLNEMSKKYGKQSFDNIEVKDITMAY
                     LLNGGYDSKGIPPVWIIERSDGEYYFLQLAKKNSAVQQKDE"
     gene            complement(2073..2867)
                     /locus_tag="PK733_06740"
     CDS             complement(2073..2867)
                     /locus_tag="PK733_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10275.1"
                     /translation="MCKKLFCTVVILILVVFMAIPVMAHPVDSLEDKSSQSSGNTDVK
                     NLDKEQEFKARFEAELLAIIENKDIKETEQFLVSFTRYVSSDIVSKKLYNVSGCTKEE
                     SISVILSRYNEDKGIYEQFLNIEGENRWDIGISGFFTKSFELKKGKNKFKIIIYKKPV
                     FDSLKEKENLAGINGEVSISDTQGTISVGATEDKNNIDVLKDLSSQNDEIFLEEGKNL
                     QIQYFTVVFLDESDKDKLLNSVTKIEDVFKSNIVDTDINGKRFQFK"
     gene            complement(2974..4812)
                     /locus_tag="PK733_06745"
     CDS             complement(2974..4812)
                     /locus_tag="PK733_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ10276.1"
                     /translation="MSFKSIQWRLVSIFILITIFLITPVGLFLNRKVETQHYDSFKSS
                     LERGFDNWLIKSDSSLKDMLSYLSVNDGRNAIIEFSILDAYKSYTVIDKNDLSVKYSS
                     DRYYSAADSNGKLFMNEIICSVNFIAVLNNELKGDKSKLVNINGRRYFDYAREVKLAD
                     GDFILYFRYDSEAWQETITGFNKIILISLLISVLISLILGYMLSKTITSPINKIMYKA
                     QRLAAGDFDQMLEVMSDDEIGQLTKAFNYMAKNLKDTLTEISSEKNKIETIFNYMTDG
                     VIAFNLKGEAIHVNPAFLKMLGQKEFNYTFNEFVEEYGLDLKLEEILYLETFSSRESS
                     ITKDGKILKAYFAMFTDEFKKAEGIIAVLHDITEQEKLENMRKEFVANVSHELRTPLT
                     SIKSYTESLLDGDIDDREIAESFLNVINSEADRMTRLVKDLLQLSRLDNKQMQWNMKE
                     ISFVELIRNSANKLQIEAKNKGHKLECYVIGDIPLVKGDRDRLEQVTINIITNAIKYT
                     PEGGKVSVYIGKTYNEAYVKVADNGVGIPEEDLPRIFERFYRVDKARSREMGGTGLGL
                     SIAREIIEAHGGTINITSELGKGTEVLVRLPAVSQDIACNSFLNTM"
     gene            complement(4839..5537)
                     /locus_tag="PK733_06750"
     CDS             complement(4839..5537)
                     /locus_tag="PK733_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ10277.1"
                     /translation="MGKKILIVDDEKHIVDILKHNLKKEGFTTIEAYDGEKGMEMGLN
                     ENPDLILLDIMLPKMDGFAVCRKLRQSVNTPIIMLTAREEEVDKVLGLELGADDYITK
                     PFSQRELIARVKANLRRTSVDEIGTGKSEGTLVCGDLNINIDRYEVVRENTVIELTLR
                     EFELVKFLAQHQGQIFTRENLLEKVWGYEYYGDVRTVDVTVRRVREKLEKDPANPQYL
                     LTKRGVGYYFYKAD"
     gene            complement(5726..6940)
                     /locus_tag="PK733_06755"
     CDS             complement(5726..6940)
                     /locus_tag="PK733_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ10278.1"
                     /translation="MLNIRSKTRQIFEVISRIEIKEKILRVNKYKAGNNFTKIICFVL
                     IFILIISISSIEIYAEDNKVIIDENISIESIGDIYKANSIQAKIDDINIELNKALYKQ
                     ATEDADKYWYTEDKYLDYNKLLIKHIRPMEALNNLKKAQKSIEENLLNLKLNIYKNFQ
                     SSSLAQKQLEAEELKLAIMFERYELAKVKRSNGLITDNELLEIEVGIENKEFDIAKIK
                     ENIKSKSEELKRLLSIEIDDNKYNINIETSFLFEPVKIDDLKTYVKSAINNNVDVYRY
                     LNDIELKQKQLDIIEQCFSVHNLSYISVRYSLEETIAALEEKKINIEVSVKNAYSNLL
                     NQRDRVYLADKYVDIMKKKLDISELKYKNGVITKDALLSAREALVNAEYQRHSAVYSY
                     NLIKADFQRLVK"
     gene            complement(6993..8126)
                     /locus_tag="PK733_06760"
     CDS             complement(6993..8126)
                     /locus_tag="PK733_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ10279.1"
                     /translation="MKKLLKNKLPLFLVMCMVLMISDIYAYASEVKSMSFEEASGLML
                     YNSRIRKNMDKIVLDMEYEYNKAVELSVNIDIYDSPTLYARMLLLNNKHLEPEQKRCS
                     WEAASQRRIITQNTLIVNLRGYYTALLGAKSNFRNKEKEYVIAVQKFKNEEVKYKNGQ
                     ISSYDLEEAEYELCKMEIERNIENRNYEKECRNFNAYIGNKLDTFYDEISFENMICFQ
                     LKDLEYYINQALNFREEINIIKWQIKLKKLEIGILEKDNVNIIYTDVKRQHSDITKEI
                     NDLELNLQKTQLAISNEIKDAYFDIKSSYRELSMMETRLNTVKRNLSVIKAKYIEGII
                     PRYRVSEEEIKIEEMEDRYNISIFNYNTKVMKFKNACLSGPGY"
     gene            complement(8300..11365)
                     /locus_tag="PK733_06765"
     CDS             complement(8300..11365)
                     /locus_tag="PK733_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ10280.1"
                     /translation="MPGRNHRKRSLIINLMIITYIFTIVFQIQVYTIGESIKTEAEIC
                     AILGVLKGDGKGITQEYLGKRTERLQAVILTLRLFGRNYEQEALEYNMNNNFIDADDI
                     TWQEGINVLAYIKANDQFGWHGGIDGKFNPRGLVTPQMFYKILLEALGYRQDYGNSKD
                     FDFKWEEVMKFAFKVGLWDIADKEVLKNEDIAVAIVEALQLKMKNSDRTLLEKLMDEG
                     AIEKEKAIEVGLLSISPQITSIKEIDLGVINIGDEVKLPQTVTATFSDESQKEVPVNW
                     ETAIENLKEGRRTIVGEVQGTEIRAFANITFISLPLEVKDVCADNLIEVNIVLNKAVD
                     VNKVKNKNNYSIIADGNKAEINDIRVSQDRKIVTLCLVNPVQQQKRVEVTVKKEVGLE
                     QDIKKTIDSIIDTKLPEVTELTVLGNKKIRIVYSEPVQNAEQSSSYSIDDSTLYGYVT
                     KIDRKTLLLNITNPLSIGTHYLRINKSVYDYSNYPIMQEPIEFKVYQDNSLPEIEKII
                     SATQTKVKIRFNKPVEPIVKEKIVTVHAAEVISVTADDDFKTYTIEFERTSALAEQGT
                     DVYFYDVTDYYGNKNTIKVYITPSIDKETPGFSGYTIKDQNKIILEFTKDVVPTGALY
                     ILKKISGETVPLTQIGWYIDEAGNIIRNKVVLQKQGDSLFEPGTYNLSIQDVVDYTPQ
                     ENRIVPLTIDISIIDDISPEIKYVRMKDNRLFILFNEKVDRITALSSENYRYLNFSNY
                     VSEKLPEGTVIELLSDYRTVVISLPEKFNMNDINVLQISAVKDEAGNVMVAKGIIAPF
                     EKVESPSRVLYAYITGRNTIELILSKDINGETLTAADFIITAGTETLSISSAEYDAGN
                     KKITLYVNDHISSNGQFGGKNVTVHTALKDIKTSDVYGQPIQGLGLTTAVDKYPPYVT
                     GMSVLNVNSNTNIIITLNENIKTANGSGKSLSSSELGQFIVLVNNTVNQVIESRYEAA
                     TNTGAARIILTISGNQVGKNIRVLFFAGPNNTLTDYAAIPNSLENFELK"
     gene            complement(11592..14738)
                     /locus_tag="PK733_06770"
     CDS             complement(11592..14738)
                     /locus_tag="PK733_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ10281.1"
                     /translation="MKRLNKLLAVLLALTLIAGITIVPTFAAEPLKYENEAKTLNQLK
                     LFKGTSETEYKPNLEARLLREESVALLLRMFGLEAEALKLTDKEAADILNAKFKDAGE
                     ISGWAVKYVAYAVKNEIIAGRPDGKFAPGENLIGREYAKMLLSMLGYKQGTDFQFEFA
                     IVELCNVTDFAKAEAVKLDTIPLLRDDVVGLSFSTLKAEYAAGANKGKTVIAVIVGDD
                     KEMQTIALDAGLIEKAVIKSLAEVAAVEIKVGDKLNLPEKVKATYSDGTEAEVAVKWD
                     VTAVKTDAAGEYKAVGTVEGFEGKLNVAVKVVSVDINVVGTTADNMKEILVSFNSELD
                     SATVVKDNFAVAGQTVKSVKLLDSKKEVLITLDGYLAPNPKEYEVTVKNVKNVTGKQI
                     AEVKVKGNAFDKIVPEAVAVKFVGPNAVEIEFSEYIDTTVTGSVTINNGIYGINSAKV
                     EGKKVKVEIGTNLPNGDYTFKVEGFKDYAGFLMPVKTFEVAYAADTSNPTVSVKEATQ
                     TYVIITFSKPVKGLTTADVYHTYTAWQPLEIQVGGAVYNPADSASEFKLIFVDALKKS
                     PDNTTAYDRPVPVGSSSLVVKKTDKLVDLWGVKLEGDATLPLNIAADLSAPEVSKVEV
                     TAEDIVKITFSKEVIAPTKDNFVIKKADGAESTTGYSITYADKVATLTFNSKLAGGVY
                     TIEIKDVKDTSLAVNKMVTVIKEITITDKTPHDTTKTKVEYVFDGDFSLTGGKNKNPY
                     TLYLYFNEKMAIEGANSVLSADNYRISYDGTTYAKLPANTSIAVFVDSSKIKITIPVN
                     SITAVPQKLLIGRVADVAGNLSDLLSYERNVSTSAAAPLIEKVQSINANTVKLILSTE
                     IKSINASKIALAEADISTLDFSQDANKKYLAGAANYVNKNGKAEVTITLKADNKLSAS
                     DALITSVVLLADNNIKSLLDIEALAGSVAGFAAEDKFAPSIKAITAKTGENEIAIQFT
                     EAVDAASVSRLTFTVKDRTVSAAVVAGDTVTLTVSGDAFVKAAKLEITQQYPITDAVR
                     NEYKADKVQTVEVKDAAV"
     gene            complement(15089..15262)
                     /locus_tag="PK733_06775"
     CDS             complement(15089..15262)
                     /locus_tag="PK733_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOJ10282.1"
                     /translation="MGRRRSIMSENLKAEIAKELGVYNTVASEGWGAVSSRDCGNIVK
                     KAIEMAERSINGK"
     gene            complement(15390..15821)
                     /locus_tag="PK733_06780"
     CDS             complement(15390..15821)
                     /locus_tag="PK733_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ10283.1"
                     /translation="MKVRDKMTKDIAYVSPNTTVTETAQLMQKHNVGSIPVLDENNLV
                     GIVTDRDIVVRNVAHGNDPGSTPVSSVMTTQVVSVTPDADVDEVIGKMSMNQIRRVPV
                     LENNMLVGILAIGDVATDRKFNMEASKALTEISKPSKPEMF"
     gene            16194..17393
                     /locus_tag="PK733_06785"
     CDS             16194..17393
                     /locus_tag="PK733_06785"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ10284.1"
                     /translation="MQNKFIKITILVIVIIATIFTLIVFFAPFSLVINKDDINVYIKP
                     VNEQIKIITADSSNRFLFMAQVRNNAGEPISGIPIVFNVDNNVGILSSKTIRTNKFGE
                     AFVTYIPPDNPLKNIPGDNPDNTNNTDKTKVNVNNIEPIVNVTVSAEVSKWNKAISRS
                     KFSFSIITVPVVLVHGYQSSGDIFSNMKDYLNSQGFNTVIHNYDSTKGVSAGAKSLKE
                     FLKEQKTLYLSKGIQLERFDIVSHSMGGIISRYYSVSSDYIEYNNIRKLIFISVPHKG
                     SHLAPIGAKYFNDAGVKDMMPESSLFTELFPSMINKGLNPYIQTGNIIGQYDEVVSIE
                     SAGLQEWRIETRVFNIGSSKFNVDNFIDGTISHEAIHKLILNNKKVYETIVDMLVRSL
                     PYPSKIN"
     gene            complement(17790..18623)
                     /gene="nadC"
                     /locus_tag="PK733_06790"
     CDS             complement(17790..18623)
                     /gene="nadC"
                     /locus_tag="PK733_06790"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOJ10285.1"
                     /translation="MINDIIIDNLIKRALEEDMPFGDITTDALIDEQSISRAEFIAKE
                     AGVIAGLDIAGRVFEHLDGRVIFTKIVRDGDKVNKGDIIAEVEGQTRALLKGERTALN
                     ILQRLSGIASKTNEICSKVKDLSVSIADTRKTIPGMRILEKYAVKTGGGSNHRYSLSD
                     GVLIKDNHIAAAGSIKKAVEAAKASVPHTIKIEVETETINQVQEALVAGADIIMLDNM
                     NEEKMREAVKLINKRALVEASGNVSLDNVRQVALTGVDIISIGTLTHSVKALDISMKI
                     R"
     gene            complement(18647..20287)
                     /gene="nadB"
                     /locus_tag="PK733_06795"
     CDS             complement(18647..20287)
                     /gene="nadB"
                     /locus_tag="PK733_06795"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOJ10286.1"
                     /translation="MNDYRFLVDFDTKNIEMDYYDVIIIGSGIAGVYTALEIPEKNNI
                     LILTKETLEISNSVLAQGGIAVSLDKGDSPELHLKDTLYAGAGLCNEESVWVLVSEAA
                     LNIEKICEYGVKFDRKSPEELAFGREAAHSKSRIIHAGDTTGKEVLDKLISVVKTKPN
                     VKIKERSFAIDLLTENNETKGVIVYDEDTSKLKIYLSHVVVCATGGYGQLYENTTNPE
                     VATGDGTCLAYRAGAELMDLEFIQFHPTVLFHPENKSFLISEAVRGEGAILKNINKER
                     FMPAYHEMAELAPRDVVSRAIFQEMKKTGSDHVYLDITFKGKEYLENRFPNIYKTCLN
                     YGIDISKDYIPVAPAEHYCMGGIKTNIHGRTNIKGFYACGEAACNGIHGANRLASNSL
                     LEGLVFGHKIGIEITDYIDEKEGKLPEYQVKYSTDRIKKDINIDTYKDINKAIDKKTV
                     KNDIKSIMTQYVGIIRNKEGLEIAKDKINRYRDLTANMRNTFIIDWEIQNMVLLSSMI
                     INSALQREESRGAHYRSDFSHTDDENWKRNIIVKKQTR"
     gene            complement(20343..21272)
                     /gene="nadA"
                     /locus_tag="PK733_06800"
     CDS             complement(20343..21272)
                     /gene="nadA"
                     /locus_tag="PK733_06800"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOJ10287.1"
                     /translation="MENNNLGSNLELKNKIMKLKKEKKAVLVAHTYQRDEVQEIADMV
                     GDSFALSKYCASSNAEVVVFCGVHFMAESAKILSPQKTILLPEKHAGCPMADMVTAEA
                     LREFKKNYPNAAVVCYINSSADVKAECDICCTSSNAVKVIKSLKEDEIIFVPDKNLGA
                     YIGEMVPEKKMILWEGYCPTHHKIRPEDVEKARELHPDAVVLVHPECRPEVFKIADFV
                     GSTKQIIDYATKSEKRKFIIGTEMGVLYSLKKNNPDKTFYMLANGFICPNMKKTTLSS
                     IYNSLNEMKYNIELDDETIKKASQSLERMLEIS"
BASE COUNT         6299 a         4242 c         2957 g         8035 t
ORIGIN      
        1 acatatctat ctaaaaaaaa cgacgatgca tttatgccca aactttcaat taactcctta
       61 gaatcttttt caatctgctt tttttctttc tcactaatat tatcattttt tattgaaaag
      121 ctctttttgt catcgttttc atcgtttgtg cccaaagctg aagggtttgc atccctgtat
      181 ataaaggaac cgctccctgt aaaaacaagg gttttttctc ctgtttcata aatatcatct
      241 tcatctgctt tatctatttt actcgtttta tcctgatcat tatcattaaa tgaaatcatt
      301 gaagcaaaat cctttccaag caatacactt acaacctttt ctttgttgat attgccattt
      361 ccaagctcca gcatgggtgt ttttttattg tacccgggta tttgacaatt ttcatcaatc
      421 cttataccct ttttctgcaa tatacttagt atactggttg cagtttcctt tggaatgctg
      481 ccctgaattt tatatatacc agaataaatt agaagaaaca aattcaatgt aataaatatt
      541 attataaata tgttttttgc tcttgaccaa tccactgcta ttcatccttc tgctgcactg
      601 cactattttt tttagcaagt tgtaaaaagt agtactctcc atcagacctt tctattatcc
      661 aaacaggtgg aattccttta ctgtcatatc caccattaag gagataagcc attgtaatgt
      721 ctttgacttc aatattatca aaactctgct ttccatattt tttggacatt tcgttcagca
      781 tgtcttcaaa atatatgtta tattcacttt gagtccggct cttctcaata cttgcaagat
      841 accacctgca ctcaattatt ctctttccgt ttgctttaat ggatactgca cttttaacag
      901 gctctttatc tccgctcttg tcttgaaaat taatataaac cggcaaatcc cccaatttat
      961 aatcaaaaat gaactcgtag taaccttgat tctcatcttt tacctgtttt aagtacagat
     1021 ctgtttcagg atcaatataa ccttttttaa tcctgctgat gaaaatataa gctttttcaa
     1081 aagccgaacc tatgtcactc ttttcctgat catccacacc gggaatatac ttatattcta
     1141 aaaggccatc attatacagt cggtatgtat tattaatatt cttgaataca accgcattat
     1201 tctcaatata gcggtcaaaa ctttcctttt catttccaag gataactctt gcaatttctt
     1261 caatatctgc agtttttcta ttatcataat aaatcgcaga tggatattta taatatttta
     1321 taccttcagc tacacacaag atatccggcg aaatagacaa ctgaagtttt ttgctaataa
     1381 cattatttgg atcaatctct ttaaaggtat tgtattgtac aatattgcca ttatactcca
     1441 ggttgttaat agtttttgta taaccgcttt ttaacattcc ctttttgtta aaaggtacta
     1501 tgtatctgta taaacccttg gaactatata tgtaaactgt attgttatta ttaatatctt
     1561 cataaggtaa aataaatatt ttctggatac tatcaaaata atcagaggaa gatccggtaa
     1621 tgtcaagaaa ccattttaaa aggcctgttt tgattcctgt tttaaactct atcataatgc
     1681 ttttttttac tgttaagtca atccatgtat tcatgttgac actatggcct tcaccggaag
     1741 ttaatacttt ttcaaggtag ccgcaggctt cgtcccacag ctcattgtaa atgtagtcat
     1801 ccttaccgat tacccagtgg gttctatttc cattggatat tgttattctg tatggactaa
     1861 atatatcttt tcttgctatc tccactatat cagtcttatc attattgctc tggctgctaa
     1921 ataccttcga tataaaatta aaaggaaacc tatgactttg ataatcccac aggattccta
     1981 cctgtattat gcttgataat actaaaacta ttaaaacagc agattttacc ttttctctgt
     2041 taaagctaag catttaagta ccttatcccc ggttatttaa actggaatct tttgccattg
     2101 atatctgtat caacaatatt acttttgaaa acgtcctcaa ttttagtcac gctgttaagc
     2161 aacttatctt tatcactttc gtccaagaaa acaactgtaa aatattgtat ctgcaggttt
     2221 ttgccttcct caagaaatat ttcatcattt tggctggaca gatctttcaa aacatcaata
     2281 ttatttttgt cttcggttgc ccctacagat attgtaccct gtgtatcgct tatactaacc
     2341 tccccattaa tacccgccag gttctctttt tcttttaagc tatcaaatac aggtttttta
     2401 taaataatga tcttaaactt gtttttgcct ttcttaagtt caaacgattt tgtaaaaaag
     2461 cccgatatcc caatatccca tctattttca ccttcgatat tgagaaattg ctcatatatg
     2521 cctttgtcct cattatacct gcttaaaatt acgctaatac tttcctcttt agtacatccg
     2581 gaaacattat ataatttctt tgagacaata tcacttgaaa catatctggt aaaggaaacc
     2641 agaaattgtt ctgtttcttt aatatcttta ttttctatta ttgctagcaa ctcggcttca
     2701 aacctcgcct taaattcctg ttccttgtcc agatttttaa catcagtatt tcctgacgac
     2761 tggcttgatt tatcctcaag actgtctaca ggatgggcca taactggtat agccataaaa
     2821 acaaccaata ttaaaatgac tactgtgcaa aataatttct tacacatttt aattctcccg
     2881 gaaacaattt attaagtcaa atagtctaat aatatttaca cttaaactat acattactca
     2941 aataaattat acattttttt tgttacaact ctattacatc gtatttaaga atgaattaca
     3001 tgctatatcc tgtgaaaccg caggaagcct cactaaaact tcagttcctt ttcccagctc
     3061 acttgtaata tttattgtac ctccatgggc ttcaattatt tcccgggcaa ttgaaagtcc
     3121 aagacctgta ccacccattt cccttgaacg agccttgtct actctgtaaa acctctcaaa
     3181 tattctgggc aaatcttcct caggtattcc tacaccatta tcagcaactt taacatatgc
     3241 ttcattatat gtttttccaa tataaactga aacctttcct ccctcaggag tgtatttaat
     3301 agcatttgtt attatgttga tagtaacttg ttcaagcctg tccctgtcac ccttaacaag
     3361 aggtatatcg cctataacat agcattccag cttatgacct ttatttttag cttctatctg
     3421 taatttattt gcggaatttc ttataagctc cacaaaggat atttccttca tattccactg
     3481 catttgctta ttatccagtc tggaaagctg aagtaagtcc tttaccagcc tggtcattct
     3541 atcagcttca gaattaatta cattaagaaa gctttcagca atttctctgt catcaatatc
     3601 cccatccaaa agagattcag tatagctttt tattgaagta aggggagttc tcaattcatg
     3661 ggaaacattt gcaacaaatt cctttctcat gttttccagt ttttcctgtt cagttatatc
     3721 atgtaatact gcaattatac cctcagcttt tttgaactca tctgtaaaca tggcgaaata
     3781 agcttttaaa atctttccat ctttagtaat gctgctttcc ctgctcgaaa aagtctctaa
     3841 atatagtatt tcctcgagtt tcaaatctag tccgtattcc tccacaaact cattaaatgt
     3901 gtagttaaat tctttttgtc caagcatttt caagaaagca ggatttacat gaatcgcctc
     3961 gccttttaaa ttaaacgcta taacaccatc tgtcatataa ttgaatatgg tttcaatttt
     4021 attcttttca ctagagatct ccgttagagt atccttcagg ttcttagcca tatagttaaa
     4081 agcctttgta agctgaccga tttcatcatc ggacataact tccagcatct gatcaaagtc
     4141 acctgcggcc agtcgttgag ccttatacat aattttatta ataggcgatg ttattgtttt
     4201 agataacata tacccaagaa taagagatat tagaacagaa atcagcaaac ttataaggat
     4261 aatcttgtta aatcctgtaa tagtttcctg ccatgcttca ctatcatatc tgaaatataa
     4321 aataaagtct ccatctgcaa gttttacttc tctggcataa tcgaaatatc tacggccatt
     4381 tatattaaca agtttgcttt tatcaccttt taactcgtta tttaagacag caataaagtt
     4441 taccgaacag ataatctcat tcataaacag ttttccattg ctatcagccg cactataata
     4501 tctgtcagat gaatatttta cacttaagtc attcttgtca ataactgtat agctcttgta
     4561 tgcatctaaa atagaaaact ctataattgc atttctacca tcatttacac ttaaatatga
     4621 taacatatcc ttcaaagaag agtcactttt tattaaccaa ttatcaaaac ctctttccag
     4681 ggatgactta aaactatcat agtgctgggt ttcaactttt ctatttagaa aaaggccaac
     4741 aggcgtaatc aaaaatattg taattaaaat aaatatacta acaagtctcc attgaatact
     4801 cttgaaagac atatatacct cacttagtca gctttcactt agtcagcttt gtaaaagtag
     4861 taccctacac ctcttttagt caataagtat tgcggatttg caggatcttt ttcaagtttt
     4921 tcccttaccc ttcttactgt cacatctaca gtccttacat caccatagta ttcatatccc
     4981 cacacttttt caagcaggtt ctccctggta aaaatctgcc cctgatgctg agctagaaac
     5041 ttaaccagct caaattccct taatgtaagc tcaattacag tattctcacg aaccacttca
     5101 taccggtcaa tattaatatt taaatcgcca cacacaaggg taccttcaga ttttcctgta
     5161 ccaatctcat ctacgcttgt tcttcttaaa ttagctttta ctctggctat aagttctctt
     5221 tgactgaaag gttttgttat ataatcatct gcaccaagtt ccaagcctaa aaccttatct
     5281 acctcttctt ccctggctgt aagcataatt ataggagtgt tgacagattg cctaagttta
     5341 cggcatacag cgaacccatc cattttagga agcattatat caagcagtat caaatctgga
     5401 ttctcattta atcccatttc catgcccttt tctccatcat atgcctcaat agtggtaaaa
     5461 ccttcttttt tcagattatg ctttaaaata tctactatat gtttttcatc atcaactatc
     5521 aggatttttt tacccaatcc aaccccccct tgattttggt tataaaccta ttataccatt
     5581 aaataaataa atttgaatga gtattttaaa acaaaattgt aatattattg taatatgttt
     5641 gtattcatct acagcataat atgattagac gtaagataag attgtttagt tccaaaaaag
     5701 cataaatttt tatgcctgaa tcacattact taactaacct ttgaaaatcc gcttttatta
     5761 aattatagct gtaaacagca gaatgccttt gatattctgc attaacaaga gcttctcttg
     5821 cacttaaaag tgcatccttt gtaattactc catttttata ttttagctct gaaatatcca
     5881 gcttcttttt cataatatcc acatacttat cggccaaata caccctgtct ctttggttaa
     5941 gcaaattgga ataggcgttt ttaacgctaa cttctatatt aatttttttc tcttccaaag
     6001 ctgcaatagt ttcttcaagt gaatacctta cacttatata tgacaaatta tgaactgaaa
     6061 aacattgctc tatgatatca agttgtttct gttttagttc aatatcattc aaatatctgt
     6121 acacatcaac attattgttt attgcagatt ttacatatgt ttttaaatca tcaatcttta
     6181 ccggttcaaa tagaaaggaa gtttctatat tgatattata tttgttatcg tcaatttcaa
     6241 tgcttaaaag cctttttaac tcttctgatt tcgatttaat gttttctttt atttttgcta
     6301 tatcaaattc cttattttca attcctacct caatttcaag caattcgtta tctgtaatca
     6361 acccatttga cctctttact tttgccagct catacctttc aaacatgatg gcaagcttta
     6421 actcttcagc ctccagctgt ttttgtgcca gtgaagaaga ttgaaaattt ttatatatat
     6481 ttaacttcag gtttaataaa ttctcttcta tagatttttg tgcttttttc aaattattta
     6541 aggcttccat tggtcttata tgttttataa gcagcttatt ataatcaaga tatttatctt
     6601 ctgtatacca atacttatca gcatcttctg tagcttgctt atataaagct ttgttcagtt
     6661 ctatgttaat atcgtctatt tttgcctgaa tactatttgc tttataaata tcgccaatag
     6721 attcaatact aatattttcg tcaataataa ctttattgtc ttctgcataa atttctatag
     6781 aagaaatcga aataattaat atgaatatta aaacgaagca aataatctta gtaaaattat
     6841 tacctgcttt atatttgttt acccttaaaa tcttttcttt tatttcaatt ctgcttatta
     6901 cttcgaatat ttgcctggtt tttgacctta tatttaacat taatacaccc ccattattcg
     6961 tgcgcatgat tctcattatt ggtatgaatt tattaataac ccgggcctga taagcatgca
     7021 tttttaaact tcatgacctt tgtattataa ttaaatatag atatattgta cctatcttcc
     7081 atctcttcta tttttatttc ttcttcacta actctataac gtggtattat accttctata
     7141 tactttgctt ttattactga aagatttctt tttactgtat taagacgtgt ttccatcatg
     7201 ctcagctctc tatacgaact ttttatatca aaataagcat cttttatttc atttgaaata
     7261 gcaagttgcg ttttttgtaa attaagttcc aaatcgttta tttccttggt aatatctgaa
     7321 tgttgccttt taacatcagt atatataatg ttaacattgt ccttttcaag tatcccaatt
     7381 tcaagctttt ttaattttat ctgccattta attatgttaa tttcttctct gaaattcaag
     7441 gcttgattta tataatactc aagatctttt agttgaaagc aaatcatgtt ttcaaaagaa
     7501 atttcatcat aaaatgtatc aagtttattt ccaatatatg cattaaaatt cctgcattct
     7561 ttttcataat tcctattttc tatattcctt tcaatttcca tcttgcatag ctcatattct
     7621 gcttcttcta aatcatatga agaaatctgg ccatttttgt attttacttc ttcatttttg
     7681 aacttttgta cagcaattac atactctttt tctttgtttc gaaaattgct tttagctccg
     7741 agtaatgctg tgtaatagcc cctaagattt actattaagg tattctgcgt aattattctc
     7801 ctttgggatg ctgcttccca gctacatctt ttttgttcgg gttctaagtg tttattgttc
     7861 aataaaagca tgcgtgcata taatgttgga gaatcatata tgtcaatatt cactgataat
     7921 tctactgctt tattatattc atactccata tcaagaacta ttttgtccat attttttctt
     7981 atgcggctgt tatatagcat aagtccactg gcttcttcaa atgacatgct ttttacttct
     8041 gatgcataag catagatatc tgatatcatt aataccatgc acataactaa aaataaaggc
     8101 aatttgtttt tcagcaattt tttcacttta tcaccacaaa ccttttattt aatcacatat
     8161 cccgaatata tgaacttaac ccattgttat ctactatttc cctcaaaagt taacataaaa
     8221 taattttttt atactacaca ctacagtgaa atcgttactt gtcacttttc aagggaacat
     8281 atcaccttat atttttatat tactttagct caaaattttc cagcgaattc ggtattgctg
     8341 cataatcagt taatgtatta ttgggtccag caaaaaacaa cacccttatg ttttttccaa
     8401 cctggtttcc gcttattgtt aatataattc tagccgcgcc agtatttgtt gctgcttcat
     8461 accttgactc tatcacctga ttaactgtat tattaacaag tacaataaat tgaccaagtt
     8521 cgcttgagct taaagatttc cctgagccat tggcagtttt tatattttca ttaagagtaa
     8581 taataatatt tgtattggaa tttacattta gtaccgacat acctgttaca taaggcggat
     8641 atttatctac agctgttgtc agtcccaggc cttgtatggg ctgcccgtaa acatccgatg
     8701 tttttatatc ttttagagca gtatgtacag ttacattttt tcccccaaac tgtccgttac
     8761 ttgaaatatg gtcatttaca taaagagtta tctttttatt acctgcatca tattcagcac
     8821 ttgaaatact aagagtttct gtacccgctg ttataataaa atcagcagct gtaagtgttt
     8881 cgccgttaat atcctttgat aagattaatt ctatagtatt tctccctgtg atataggcat
     8941 aaagaactct tgaaggagat tccacttttt caaacggagc aataattcct tttgcaacca
     9001 ttacattacc tgcttcgtct tttacagccg aaatctgtag aacgtttatg tcattcatat
     9061 taaatttttc agggagagat atcacaactg ttctataatc tgataatagc tcaatcacag
     9121 ttccttcagg aagtttttcc gatacataat tactgaagtt caaatatctg taattttcac
     9181 tagataaagc agtaatccta tcaacttttt cattaaataa tataaatagt ctgttatctt
     9241 tcattctaac atattttatt tcaggactta tatcatcaat tatgcttata tctatagtaa
     9301 gtgggactat tctattttcc tggggagtat agtccactac atcctgaatt gacagattat
     9361 atgttccagg ctcaaacaaa gaatctccct gtttctgtaa taccacttta ttccttataa
     9421 tgtttcctgc ttcatctata taccatccta tctgggtaag tggtactgtt tctcccgaaa
     9481 tttttttaag tatatataaa gcacctgttg gtactacatc ctttgtgaac tcaagaatga
     9541 ttttattctg gtcctttatt gtgtaacctg aaaaacctgg tgtttcttta tcaattgacg
     9601 gggtaatgta tacttttata gtatttttgt tcccataata gtctgtaaca tcatagaagt
     9661 atacatccgt gccctgctca gctaatgcag atgtccgttc aaactctatt gtatatgttt
     9721 taaaatcgtc atcagctgta actgatatga cttctgcagc atgaacagta acaatttttt
     9781 ctttaacaat aggttctacc ggtttgttaa accttatttt tactttggtc tgtgtagcac
     9841 ttattatctt ctcaatttca ggcagtgaat tatcctgata tactttaaat tctattggtt
     9901 cctgcataat aggataatta gaataatcat atacactttt attgattcta aggtaatgag
     9961 ttcctatact taaaggattt gtaatattta gtaataatgt tttcctgtcg attttcgtta
    10021 cataaccata taatgtagaa tcatctatac tgtaactaga tgattgttca gcattttgca
    10081 caggttcaga atatacaatc cttatttttt tattgcccaa cacagttaat tctgttactt
    10141 ccggaagttt tgtatctatt atgctatcga ttgttttctt tatatcttgc tccaaaccaa
    10201 cttccttttt aacggtaacc tctactctct tctgctgctg gaccgggttt acaagacaca
    10261 atgtaactat ttttctatct tgcgatactc tgatatcatt aatttcagct ttattgccat
    10321 ctgcaattat actataatta tttttatttt ttactttatt tacatcaact gctttgttta
    10381 acactatatt aacctctatt aggttatctg cacaaacatc ctttacttca agaggaagtg
    10441 aaataaaagt tatattggca aatgctctta tctctgttcc ctgcacctct ccaactatag
    10501 ttcttcttcc ttcttttaaa ttttcaatag ctgtttccca attaacaggt acttctttct
    10561 gactctcatc actaaaagta gcggtaacag tttgaggcaa tttaacttca tcacctatat
    10621 taattacacc aagatctatc tcttttatag atgtaatttg tggtgaaata cttaataaac
    10681 cgacttcaat agctttttct ttttctatgg ctccttcatc cataagtttc tctaaaagtg
    10741 ttctgtcaga atttttcatt tttagctgca gagcttcaac aattgcaact gcaatatcct
    10801 cattttttag aacttcttta tccgcaatat cccacaatcc tactttaaat gcaaatttca
    10861 taacttcttc ccatttaaaa tcgaaatctt tactattacc ataatcctgc ctgtatccta
    10921 aagcctcaag caatatttta taaaacattt gtggtgttac cagtccacgg gggttaaatt
    10981 tgccgtctat tcctccatgc catccaaatt gatcgttagc ttttatataa gccagaacat
    11041 ttataccttc ttgccaggtt atatcatccg catcaataaa attattattc atgttatact
    11101 ctagtgcttc ctgttcataa tttctgccaa aaagacgtaa tgtaagaata accgcctgta
    11161 acctttctgt ccttttccct aggtattctt gcgttattcc ttttccgtca ccttttaata
    11221 ctccaagaat agcacatatt tcagcttctg tttttatact ttctccaata gtgtaaactt
    11281 gtatctggaa tactattgta aatatgtatg taataatcat taagttgata atcaatgatc
    11341 tttttctgtg atttctaccg ggcataaact taccccccca atacattttt ctttggtaaa
    11401 tatttatata gtatattaat tataccatca ataaataata gcgtcaattt taaagatttt
    11461 gatgcagatt atttttagac ttattattct atgcttttgt tccgtatata caaatcatat
    11521 tctatttgtt ttatggcatg aaaaaagagc ctgggataga cccaagctct ttaattattt
    11581 attacatttt attatactgc tgcatcttta acttctacag tctgaacttt atcagctttg
    11641 tattcgtttc ttactgcatc agtaattggg tattgctgtg ttatttcaag cttagctgct
    11701 tttacaaaag catcgccgct tactgtaaga gttactgtat ctcctgctac aactgctgca
    11761 ctaacagtcc tgtctttaac agtaaatgta agcctagata ctgaggctgc atcaacagct
    11821 tctgtaaact gaattgctat ttcattttcg ccagtcttag cagtaattgc cttaattgaa
    11881 ggagcgaatt tgtcttcagc cgcaaaaccc gcaactgaac ctgctaatgc ttcaatatca
    11941 aggagtgact taatattgtt atcagcaagt agtacaacgc ttgtaatcag agcgtctgaa
    12001 gctgaaagct tgttatcagc ttttagtgta atagtaactt cagctttacc gttcttgttt
    12061 acatagttag ctgcacctgc aaggtatttc ttgttagcat cttggctaaa atctaatgta
    12121 gatatgtcag cctctgcgag agcaatctta gatgcattga tggacttaat ttctgttgag
    12181 agtatcagct taactgtgtt cgcattaatg ctctgtacct tttcaattaa aggagctgct
    12241 gccgatgttg aaacatttct ttcgtaggag agcagatctg aaaggttgcc tgctacgtct
    12301 gcaactctac ctatcaagag tttttgcggc actgcagtta ttgagttaac aggtattgtt
    12361 atcttaattt ttgatgaatc cacaaataca gctatgcttg tattagcagg gagttttgca
    12421 taggtagttc catcataact aatacgataa ttatcagcac ttagcactga gttcgcacct
    12481 tcaatagcca tcttttcatt gaagtataaa tataaagtgt atgggttctt gttcttgccg
    12541 cctgttaacg aaaaatcacc atcaaacaca tactcaactt ttgttttagt tgtgtcatga
    12601 ggagttttat cagtaattgt tatttctttt attacagtta ccattttgtt aacagcaagg
    12661 cttgtatctt tcacgtcttt tatttcgata gtgtatacac cgccggctaa tttgctgttg
    12721 aatgtcaatg ttgcaacttt gtctgcataa gttattgaat agcctgtggt tgattcagct
    12781 ccatcagctt ttttaattac gaagttatcc tttgtaggtg caataacttc tttactgaaa
    12841 gtgattttaa ctatatcttc agcagttact tctacttttg atacttcagg agctgataga
    12901 tcggctgcta tattaagagg aagtgtagca tctccttcaa gcttaacacc ccaaaggtca
    12961 acaagtttgt cagttttctt aacaacaagg ctggatgatc caacgggtac aggtctgtca
    13021 taagcagttg tattatccgg tgatttcttg agagcatcta caaatataag tttgaattcg
    13081 cttgccgaat cagcaggatt atatactgct ccgcctacct gaatttcaag tggctgccat
    13141 gctgtataag tgtgatatac atcagcagtt gttaaacctt ttacaggttt ggagaaagta
    13201 attataacat aggtctgagt tgcttcctta actgaaacag tcgggttgct tgtgtcagca
    13261 gcataagcta cttcgaaagt ttttacaggc attaagaatc ctgcatagtc tttgaaacct
    13321 tctaccttaa atgtatagtc accatttgga agattagttc caatttcaac ttttactttc
    13381 tttccttcaa cctttgctga gttaatgcca tatattccat tgttaattgt tactgaacct
    13441 gttactgttg tgtcgatgta ttcactgaat tctatttcaa cagcatttgg accaacaaac
    13501 ttaacagcta cagcttcagg aactattttg tcaaatgcat ttccttttac ttttacttca
    13561 gctatctgtt ttccagtaac attctttaca ttctttacag ttacttcata ctctttcggg
    13621 tttggtgcaa gatatccatc aagagtaata agaacttcct tcttgctgtc aagaagtttt
    13681 actgatttta ctgtctgtcc tgctactgca aagttatcct ttactacagt agctgaatca
    13741 agttcactat tgaatgatac taagatttct ttcatgttgt ctgctgttgt accaacaaca
    13801 ttaatatcaa ctgatacaac tttaacagct acattaagtt ttccttcaaa tccttcaaca
    13861 gtaccaacag ctttgtattc gcctgctgca tcagttttta ctgcagttac atcccatttt
    13921 acagctactt ctgcttctgt gccgtcgcta taagttgctt ttaccttttc aggcagattc
    13981 aatttatcgc caactttaat ttctacagca gctacttctg ctaatgattt tataacagct
    14041 ttttctatta agcctgcatc aagtgcaatt gtctgcattt ctttatcatc gccaacgatt
    14101 actgcgataa cagttttacc tttgttagcg cctgcagcat attcagcttt cagtgtagag
    14161 aaggataatc caacaacatc atctctgaga agtggaattg tatccagctt aactgcttca
    14221 gctttagcga aatcagttac attacataac tcaacgattg cgaattcaaa ttggaaatct
    14281 gttccctgct tgtaacctaa cattgagaga agcatttttg catattctct tccaatcaag
    14341 ttttccccgg gtgcaaattt cccatccggt ctaccagcaa taatttcatt ttttacagcg
    14401 taagctacat attttacagc ccaccctgag atttcacctg catctttgaa ttttgcattc
    14461 aaaatatcgg ctgcttcctt gtcagtaagc ttaagagctt cagcttccag accaaacatt
    14521 cttaataaaa gtgctacact ttcttctctg agaagacgtg cttcaagatt aggtttatat
    14581 tctgtctcgg aagtaccctt aaagagtttc agctgattta atgtttttgc ctcgttctca
    14641 tatttaaggg gctctgctgc gaatgtcggt actattgtaa tgccagcaat taatgtcaaa
    14701 gctaataata ccgccaatag tttattgagt ctcttcataa tcctcaaatc ctccttgttt
    14761 ttttttgggg aaaagtagtt gttttatttt aggtgggtta cccctctccc ctaactgaaa
    14821 ataaccctgc ctttttacct tatgattcat tgtaggcggg actcgcctat cgtgaagtca
    14881 aagaaattat atcatcgagt gtaaattcag tcaacagcta tacaggaagt gtaaataatt
    14941 tgtaacagaa ctgaaataaa gactttgcaa gtttacaaaa aaagagccgg ctgatgccgg
    15001 ctcttttttt gcctgttcag agttatatag ttttcgagac tattattata tcattttaaa
    15061 gactactatt tcccgattat cgaagcggtt atttcccgtt aatacttctt tctgccatct
    15121 ctattgcctt cttaacaata tttccgcagt ctcttgaaga taccgcgccc caaccttctg
    15181 aagcaactgt attgtagacc ccgagctctt ttgctatttc agctttaaga ttttcagaca
    15241 ttatactcct gcgtctaccc acagttcatt ccccctttca ggctataaat ttgtgcactt
    15301 atataatttc gtacacatat attattttct tcagatttta ttttttaatg tcctgtaaat
    15361 atttcaatat tagttattaa aaaatcattt tagaacattt caggttttga aggtttggat
    15421 atttctgtca atgctttaga tgcttccata ttaaattttc tgtctgttgc aacatcccct
    15481 attgccaata tacctacaag catgttattt tcaaggacag gcactctcct gatttgattc
    15541 attgacattt tacctattac ttcatctaca tccgcatccg gagttacaga taccacctgt
    15601 gtagtcataa ctgagctaac cggtgtagaa cccggatcat tgccatgagc tacatttctt
    15661 acaactatat cccggtccgt tacaattccc accaggttat tttcatctaa tacgggaatt
    15721 gaacctacat tatgtttctg cataagctgt gctgtctcag ttactgtagt gttcgggctg
    15781 acatacgcta tatctttggt cattttatct cttactttca taaatttcac actcctttat
    15841 tcttttttaa ttttaagttt tgtttttata tactatctgc ttctgtaaat ttgcaaatat
    15901 aatgtacagg tataatttaa aattaccatt taaaaacctg ccatactcag catatattat
    15961 tttgtcctgt aaaataaaaa taattagagt ttaattaaag taattaatcc attattcacc
    16021 tttcaacaaa ttttattgcc aaatattaca gctttaactt ttatcaaaaa aaatttaaaa
    16081 tattaattat gaatacatta agagctttaa tataaaacac aaataatata ctaataatat
    16141 aagaaggaat tccactgttt ataaaacata ttttacaaat aggaagtcag ataatgcaaa
    16201 acaaatttat aaaaatcaca atattagtaa ttgttattat tgcaacaatt tttacattaa
    16261 tagttttttt tgcgcctttt tcattggtaa ttaataagga tgatattaat gtatatatta
    16321 aacctgttaa tgaacagatt aagattatta cagctgattc ttcaaataga tttttattta
    16381 tggctcaagt acgaaataat gcaggagaac caatatcagg aatccctata gtatttaatg
    16441 tggataacaa tgtgggtatc ctttcttcca aaactataag gacaaataaa tttggtgaag
    16501 cgtttgtaac atatatacca cccgataatc cattaaaaaa tattccaggt gataaccctg
    16561 ataatactaa taataccgat aaaactaaag tcaatgtaaa taatatagaa ccaattgtca
    16621 atgttacagt ttctgcagaa gtatcaaagt ggaataaagc catatccaga agcaaatttt
    16681 cctttagtat tataactgta cctgttgttt tggtgcatgg atatcaatca tcgggagata
    16741 ttttctctaa tatgaaagat tacctcaatt ctcaaggttt taatacagtt atccacaatt
    16801 atgactcaac aaagggtgta agtgcaggtg ccaaaagttt gaaagaattt ttgaaggaac
    16861 aaaagacttt gtatctttca aaagggatac aattagaaag atttgatatt gtttctcaca
    16921 gtatgggtgg tattatttca agatactatt cagtttccag cgattatatt gaatataata
    16981 atataaggaa acttattttt atttctgttc ctcataaagg ttcacacctt gctcccattg
    17041 gtgcaaaata tttcaacgat gctggagtaa aggatatgat gcctgaaagc agcttgttta
    17101 cagaattatt tccttcaatg attaataagg gacttaatcc gtacattcaa acaggtaata
    17161 ttattggaca gtatgatgaa gttgtaagta ttgaaagtgc agggttacag gagtggagaa
    17221 ttgaaacacg ggtctttaat ataggcagca gtaaatttaa tgtggataac tttattgatg
    17281 gcactatttc ccatgaagct atccacaaac ttatcctcaa taacaaaaaa gtgtatgaaa
    17341 ctattgttga catgcttgtc agaagtctgc catatccctc gaaaatcaac tagttatcaa
    17401 caacctttta tgatggaaaa cataattatt aacatgaaat caacaacaaa atagtagaaa
    17461 aaacagaaaa accaacaagt tatgcacaat gatgtggata acttgttggt ttttttattc
    17521 ctgcaaaccc tttgtttata aggctttgcg taatcgctct tatgtatact gtaaatgttg
    17581 tgaaatatct gtggattgtt ttctgtttta tccacaggat gtcattacat cctaatagca
    17641 taatcagtgt ttgagcaaaa taactacaac tatcatttga tcaagtattt tgtaagtttg
    17701 attttgcctg caggtgaatt taaaggcttt aggcaccata acaatagccc ctgacaaata
    17761 tttcttcaaa ttttaaaata caccctatat cacctgattt tcatactgat atccaaagct
    17821 tttaccgagt gagtaagtgt tcctatagaa ataatatcca cacccgtaag agcaacttgt
    17881 cgtacattat ccagggatac attgccagat gcttcaacca gagctctttt atttattaac
    17941 ttaacagctt ctctcatctt ttcctcattc atattgtcaa gcattattat atcggcccct
    18001 gcaacaagag cttcctgcac ctgattaatt gtctctgttt ctacttctat ttttatagta
    18061 tggggtacag atgcttttgc agcttcaacg gcttttttta tacttcctgc tgcagcaatg
    18121 tgattatcct tgattaatac accatcagaa agagaatatc tgtggtttga accaccaccg
    18181 gtttttacag catacttttc caatatcctc attcccggaa tggtttttct ggtatcagca
    18241 atactaacag ataaatcctt aactttgctg cagatttcat ttgtctttga tgcgataccg
    18301 gaaagtcttt gtagtatatt caatgccgtc ctttcacctt ttaacaaagc tctggtttgt
    18361 ccttcaactt cagctattat atcccccttg ttgaccttat cgccatctct aactattttt
    18421 gtgaatataa cccttccgtc aagatgctca aaaacccttc ctgcaatatc caggcctgca
    18481 ataactcctg cttctttggc tataaactca gctcttgaaa tcgactgctc atcaattaat
    18541 gcatccgtgg ttatatcccc aaacggcata tcttcctcta aagcccgctt tataagatta
    18601 tcaattataa tatcatttat catataaaat ctccctgtat aatatttcac cttgtctgct
    18661 ttttaacaat aatatttctt ttccaatttt catcatcagt atggctaaaa tccgaacgat
    18721 aatgggcccc tctgctttct tccctttgaa gggcagagtt aataatcata cttgagagca
    18781 ataccatgtt ctgtatttcc cagtcaataa tgaatgtgtt tctcatattt gcagttaaat
    18841 cacggtatct attaatttta tccttcgcaa tttccaagcc ttccttattt ctgatgatcc
    18901 ctacatattg agtcataatg cttttaatat catttttaac tgtcttctta tctattgcct
    18961 tattaatatc tttatatgtg tcaatgttta tatctttttt gattctgtcg gtagaatatt
    19021 tcacctgata ttctggcagc ttgccttcct tttcatcaat ataatctgtt atttcaatac
    19081 caattttatg cccaaaaact aaaccttcga gaagtgaatt gcttgccagt ctgttggctc
    19141 cgtgaatacc attacatgca gcttctccgc aagcatagaa accttttatg tttgtgcggc
    19201 catgaatatt agtttttatg cctcccatgc agtaatgctc agcaggagca acaggaatgt
    19261 aatcttttga tatatctata ccataattca aacatgtttt gtaaatatta gggaatcgat
    19321 tttcaagata ttctttccct ttaaaggtta tgtcaagata aacatgatct gagccggttt
    19381 tcttcatttc ctggaatatt gctcttgata ctacatccct tggtgcaagc tcggccatct
    19441 catggtaagc cggcatgaat ctttccttat ttatgttttt gagaatagct ccttctcctc
    19501 ttactgcttc agaaatgaga aaacttttgt tttccgggtg aaataaaaca gtgggatgaa
    19561 attgaataaa ttccagatcc attaattctg cacctgctct atatgcaaga caggttccat
    19621 cccctgtggc aacttcaggg ttggtggtgt tttcatatag ctgaccatat ccacctgtag
    19681 cacatactac aacatgggat aaatatattt taagttttga tgtatcctca tcatatacaa
    19741 ttacaccttt tgtttcatta ttctctgtta ataaatcaat tgcaaaagac ctttctttta
    19801 tttttacatt aggctttgtt ttaacaactg atataagctt atcaagtact tctttccccg
    19861 ttgtatctcc tgcatgtata atcctgcttt tgctgtgggc tgcttctctt ccgaaagcca
    19921 gttcttccgg actttttcta tcaaacttta cgccatattc acatattttt tcaatgttca
    19981 gtgctgcttc tgaaacaaga acccacacag attcttcgtt gcaaagtcct gctcctgcat
    20041 agagtgtatc ctttaaatgc aattccggag agtctccttt atcaagagat actgcaattc
    20101 caccctgcgc aagtacagaa ttgctaattt ccagagtttc ctttgtgaga attaatatgt
    20161 tatttttctc cggtatttcc agcgcagtat acactcctgc aataccactg ccaataataa
    20221 ttacatcata ataatccatc tctatgtttt ttgtatcgaa atcaactaaa aacctgtaat
    20281 cattcacatc tattcccctc ttttcccgtc ttataactca agttaactca agtaatagga
    20341 tgctaactta tttccagcat tctctccagt gattgtgaag cttttttaat agtttcatca
    20401 tcaagttcaa tattatattt catttcatta agagaattat aaatactgct taatgtggtt
    20461 tttttcatat tggggcaaat aaagccatta gctaacatat aaaaagtttt atcagggtta
    20521 tttttcttaa gactatataa tactcccatt tctgtcccta ttatgaattt ccttttttca
    20581 cttttcgttg cataatcaat tatttgtttt gtgcttccaa caaaatcagc tattttgaat
    20641 acttcaggcc tgcactccgg atgtacaagc acaactgcat caggatgcag ttccctggct
    20701 ttttctacat cttcaggtct tattttatga tgggttgggc aatatccctc ccataatatc
    20761 atcttctttt ccggcaccat ttccccaata taagcaccaa ggtttttgtc aggaacaaat
    20821 attatttcat cctcttttaa agattttatt actttaacag catttgatga tgtacagcaa
    20881 atatcgcatt ctgcttttac atcagcggag gagttaatat agcaaaccac tgccgcattg
    20941 gggtaattct ttttaaattc ccgtaatgct tctgctgtaa ccatatccgc catagggcat
    21001 cctgcatgct tctctggtag taatattgtc ttctggggag aaagtatctt agcgctttca
    21061 gccatgaaat gcacaccaca gaataccact acttccgcat tagatgaagc acaatatttg
    21121 ctaagtgcga aggaatcacc taccatatcg gctatttcct gcacttcatc ccgctgatag
    21181 gtgtgcgcta ccagtacggc ctttttctcc ttttttaact tcattatttt atttttcagt
    21241 tctaagttgc tgcccaaatt attattttcc atatcttgaa gcacaccttc cattatcaaa
    21301 tattaattaa taatatacca ttatatttta atttagtaaa tgaaaatcga taaattcatg
    21361 gtgacacccc tctccccagc aagaggtatt ccatttacgt tgaggagtgt tcccatttaa
    21421 gatacttgtt tcatattatc ttaagcatta gtttaagata atatgaaaca aggctctgag
    21481 ctatttgctc agagccttgt tataaatccg gcagggacct actttcccag gac
//
LOCUS       DAOSDD010000054        21355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1069_length_21355_cov_5.466854, whole genome shotgun sequence.
ACCESSION   DAOSDD010000054 DAOSDD010000000
VERSION     DAOSDD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21355
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_21355_cov_5.466854"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK733_06805"
     CDS             <1..466
                     /locus_tag="PK733_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10288.1"
                     /translation="RHLFILETPNVFKHPETGYMEEEKSNLNKIENLIKEKFPNSDTA
                     KINKFPNTSQMEQTITKTMNYNSIIIVTYNRTSGYTGSADATRRFLALINAVAHKLSA
                     IIIFGNPFPAREYPRTPRLIIGYENGSCEEEVINVLTGESIAKGKLPIDLVR"
     gene            566..1915
                     /locus_tag="PK733_06810"
     CDS             566..1915
                     /locus_tag="PK733_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ10289.1"
                     /translation="MDYIIEPAKRIPIDNEVDICVLGGSCTGLFAAVRAARLGAKVAI
                     VEKQNCFGGVATSGLVNIWHSLYDTLREKQIIGGLTYETIQRLNKREAIEYIGKNKTG
                     PFVLNTEELKIELDELAKSSDIKIYFHTLYTAPYIEDGELKAVFIENKSGRKAIKAKI
                     FIDATGDGDLCASLGVPYTIPDKKQPPTTSAKIYRLHEKLSRKDFSNLIIEYGREFGL
                     EEDWGWAGGIPGIKELTFHAETHVFNVNCADADQLTYSEIEGRRQIRAYMDIVRKYGP
                     DEVKPHLVALASYIGVRETRHFCCKYRLTEEDLLYGRKFNDAVANGSYRVDIHHEDKK
                     GITLKNLDGREVYIRRGTPDVVTRWRKDDGYTNYYQIPYRCLVPNGHFDNLLVCGRMI
                     DADKGSFGAIRVMVNLNQTGEAAGVAAYLALNNNSSVKELNTDTIRKMLKQGGSIIF"
     gene            2058..3032
                     /locus_tag="PK733_06815"
     CDS             2058..3032
                     /locus_tag="PK733_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10290.1"
                     /translation="MSKETKRTGSIMIKANNNVDFIELRPQHLLCILCMNGGAYLPIM
                     EKHNLKEKLEKIKSNPDIHIRLVTGFDEIGVPTDIFYELTPQERKRDLSILQKLGLVP
                     NSIRTARILIQRINENITNLHEICVTNCMPTDTWRDCSLASAGYFDEGKKDIISTRDS
                     NEIIKAKGDSANVVNETEQLFVRAHHLLCILCSIGANTDKPKIQNNTYEVWKKIIDNP
                     DIPVTIVEGCSQCMICKPCKNYRADRSICVSLSGLRDRLKDLTVFQKLGLNPGDTIPA
                     KVLYKMIAEKIPDTFGICQYERETSPEWRNCMSLGVYEKGKNKIMELL"
     gene            3141..4034
                     /locus_tag="PK733_06820"
     CDS             3141..4034
                     /locus_tag="PK733_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10291.1"
                     /translation="MLKKIDIHAHSIRIKGINFYKSKITLATPDEVAEVYARIGVEKG
                     ILLPLINTECGGRMQGNEDAIEIVEQYPDLFYWFCNIDPRMGLNSTKTDLSFYLEYYK
                     ERGAKGVGEIGVNMYFDDPFMENLFYHCQKNQMPILFHSSTCIGGIYGLADEVGLPRL
                     EKELGKFPDLAFIGHAPPFWSHISNDLTAENWGNWYYPNTKVIPDGRLAKLMRKYPNL
                     YGDISAGSGSNAMLRDPEFSYDFIEEFQDQLLYGTDISSNKFNLKISEWLDEAVQNGK
                     ISYKAYEKVSRLNALKLLKEI"
     gene            4118..5833
                     /locus_tag="PK733_06825"
     CDS             4118..5833
                     /locus_tag="PK733_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10292.1"
                     /translation="MKNIIRIKNNYLISKALVLVILLLTLLSSCNSGKIDVSYNEKSI
                     NEKTNEASDSATVETDSHKNVTSIINPIVEEKITLKMLCQANQDLNNDMPVLQELERL
                     TNVHLEFEILPAVSAERWERFAAAMAAREPFDLVTYQYRNDINKYGMEGVFVPLNNLI
                     KEHAPNLYEKLKDPLGNEPVPYKTNVLQDITAPDGNIYNVPLISTANAIGAVWAIRKD
                     WLDKLGFQSPGTIDELYEVLKAFKKNDPNGNGLSDEIPLGITGEGTLVYSLLPLINGF
                     GAHITLYLDKTDDTIKYGPVESVWKEGMLFLTKLYKEELIDPDWTTSSKDGWIAQVST
                     NQLGMMYTWPISGIGAANIELMKIDKNYHFIAIPPFKAPNGDRFKDTSTAGNIVVPRT
                     AITATNKYPVETIKYLDFLFTDAGNRVLRYGIAGIHYDMVNGKPVVKKSLLTDTTGQA
                     AKDGIDIAPLPYISLWEKGDQFKAVDPNFNPEEDAHYIYRQPGMVEAPLPALQFLDSE
                     LYKGPNAFKAINSYAYSKWTPFITGEESLEKHDDYVKQIKTLGIDEVLKIYNSAYKRY
                     KQLGK"
     gene            6133..7782
                     /locus_tag="PK733_06830"
     CDS             6133..7782
                     /locus_tag="PK733_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10293.1"
                     /translation="MKNKKFLCLTCLGLMLTMLFTSCVVKESGTTPNTISPGTKEAES
                     EKFEPLLKEKITLKMLCQANQALSNDMPVIQELERLTNVHLEFEILPAVSAQRWERFA
                     AAMAAREPFDLVMYQYKNDLAKYGMEGVFIPLNDLMKKHAPYLYQKLKDPLGDAVVPY
                     TTNVLPEITAPDGNIYNIPLISPANAVGAIWGIRKDWLDKLNLKMPETTDELYEVLKA
                     FKVNDPNGNGLPDEVPLGCTGGGTLVYNFLPLLNGFGAHITLYVDKADDTIKYGPIES
                     VWKDGMMFLNKLYKEELMDPNWTTATANTYNAQVSGNQLGLMYAWPGSGLGNATKELQ
                     KIDPSYRFVPMPPLKAPNGDRFKDTSTAGQIVVPRTAITATNKYPVETIKYLEFMFTE
                     EGDRCIRYGIEGVHYDMVNGKPKIKQYLLDDKTGLGGKDGIDMAPLGFLSLWDRQAQY
                     DANNPNYVKEEDPYYVYMQPGTVEAPMPSLIFMDSELEKGPDAFKAINSYAYSKWTPF
                     IVGEEPLDKYDDYVAQIKTLGIDEVLRIYNKAYGRYRLSGK"
     gene            7949..10948
                     /locus_tag="PK733_06835"
     CDS             7949..10948
                     /locus_tag="PK733_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10294.1"
                     /translation="MKHIYIYRYIYKHIYKNIYKNLCFLIAVILLITLVPADSQAEPD
                     LNLKLSEKFKTNKTTTNSNNNSDNNAESGIYSGVLIDKENSYFDIQEEYKEKGYRPAS
                     DIEIFLDMQSITSNSDENAPILGEVAGKKDVLVWEEDKEYFEWTAEIPQDGLYEVLVI
                     YYPLGGTSNTIQRSIMIDGEIPFLEAYNIYFNRIWRDREEPKVNNVGDEVRPRQIEEK
                     IWQSAILTDHQGMYSEPFKFYFTKGAHKIRLIYVDEAIAISDIVVRTPGRIPSYDEVL
                     DEYNQKGYKDASQTFRFEAESNVVRKSDPTIRRESDNDPITYPASATNRKLNIMGGWR
                     WKTGNQSITWKFSVPEDGLYKIGMRIGQWYGDGLPVYRQVAIDGKVPFDELKEYKFQY
                     NKNWEINVLGDSNEGAYLFYLEEGEHELTMTVKMGTLRGIIDSIYEDSLLLSKVIRQI
                     IMLTGSEPDINFEYDLDKNIPDLLENLTKLSVNMQGKAELLNKISTKSPAMANNFLMI
                     SDQMEKMVKKPETIPLKLNDLNNALNNLGIWILSLQDHPLAIDYFIAGPSDEKLENKK
                     ANILQKLAATWQNFIISFTKDYDNIGSVYNDQIETNEVIDVWVARGREWAEIIKEMAD
                     ESFTPNTGISVKINVIPSVDTLNALLLSICSGKAPDVACSVQSNYPFEYAVRDAVVDL
                     TQFDDFKQVSGHFLPKIMIPFQYNGGVYALPETMNFKALFYRKDIIEELGIEIPDTWD
                     ELYQHVLPVLYQYGMQFHYPSTDFGPLLFQKGGEFYKDEGKKSALDSSEAYQAFREFT
                     ELFTNYGIPISANFFNRIRQGEMPMGIGDYYTYVQLSVAAPELSGRWDIAPLPGHITE
                     DGEINRFTDGIAESADIIMNQSDKKKESWEFLKWWTSDETQAKFGSELEALMGAQARW
                     NTANTAAFTNLPWNKQHLKVIQKQWEWATEAPSVLGGYFTQRHTKNAWNRVVIGGMNV
                     RDSMELAVKDINRELRNKQVEYGFIDE"
     gene            11038..11997
                     /locus_tag="PK733_06840"
     CDS             11038..11997
                     /locus_tag="PK733_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ10295.1"
                     /translation="MNNVVETINEQKVTIKASLGFGKKFKAFWGKYGIGYLFLSPYLI
                     LFTIFIIIPVVVAIGTSFTNYNMLQRPKWLGISNYRLLFTDDDIFLIALQNTAIFALI
                     SGPAGYIMSFFAAWVINQLKFKKVFALAFYAPSITSGVAMTVVWMWFFASDRYGMINN
                     VLINLGIINEPVLWNLDPATILPVVIFISVWMSMGTGFLVFLAGLQSISRELYEAASI
                     DGIKNKVQELWMITLPLMKPQLLFGAINSIVASIGVFDITKSVAGMPSPNYAAHTIVA
                     HLYDYAFLRFQMGYSSAIAVVLFSITFILGRVCMRIFSSKDIY"
     gene            12017..12904
                     /locus_tag="PK733_06845"
     CDS             12017..12904
                     /locus_tag="PK733_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10296.1"
                     /translation="MAKLYIANKRSKKKKLKRMTKGWAFVYIFMIALVAFTSMPLIYV
                     ISTAFKPMDELFVFPPRFFVRRPTIQNFTDLLISLGSATVPFTRYLFNSLFTVTVTVF
                     ITVVISSLGAFALVKYKLPGGKLIFSIILAALMFSHHVTQIPNYMVVNSLGIINTYVV
                     LIIPKLAVAYNFFLMKQFIEQFPNELLESGRMDGAKEWGLFWKVVMPSLKPAWSTLVV
                     LSFVSNWNDYFTPLVFTTSEAMKTLPYALNTISSGKAVARVGATSAAVFIITVPTIII
                     FTAMQSQVMETMVYSGIKA"
     gene            12917..14410
                     /locus_tag="PK733_06850"
     CDS             12917..14410
                     /locus_tag="PK733_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOJ10297.1"
                     /translation="MRAFGEMDTTNWLRRLIRIRRKRGIRIIRAVNRLLLLIVIFTLC
                     FSQSAKAERQQYIWENDWRIPIPTSYRNVKVITYLGEKSGFLNGAEDLFIDKNDCMYV
                     ADTLNNRVLKLTLEGETLEVFTGPSEKPLKNPKGIYVDDDGHMFIADTGNLRIIHLSR
                     KGEFIEEFIKPESNLLSEDFTFDPSKVYINSTGYIFIIKSQAFFSMGANNEFQGYIGS
                     TKVGFDFQRILINMFASDFQKSQVIKKLPDSYSNFVIDDYGMIYATVINAKRGQIRKI
                     TSAGKNIYPGKFYGEITRSGWRSKIPNFVDIAVDKNGIVSVVDQASGKIYQYDQEGNM
                     LAAFGGLGNQKGTFKIPSSIVLDSQGRLYVLDQKANNIQIFEPTNFINLVHEASRLYN
                     DGRYAESRDVWEKVLQIDGKYALAHRGIAKALIKEEKWIESMETYKLANDQEGYSEAF
                     IQYRHSIYRRYFGWIILLIAVVIVVLFYMLKYLKKLSESVLRKHMGW"
     gene            14426..15049
                     /locus_tag="PK733_06855"
     CDS             14426..15049
                     /locus_tag="PK733_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ10298.1"
                     /translation="MNVFEMSNAILFHPVDTFEYIKSRQKSISIISCILLLFAVVVVR
                     IASIFIVHYPIASIQPRDANIWLEIVKLLLPVLTWVISCYAITAISDGETLLREIFSA
                     AVFSMIPYIVCTIPLAIITRVMGRDQLQLYNTLNNIILIWVLIIIFLSVRTLNDYTIV
                     QTVKVCIISFITMLLIWAVLTLIFALTSQLIQFLCNIVRELRMIVIE"
     gene            15073..17304
                     /locus_tag="PK733_06860"
     CDS             15073..17304
                     /locus_tag="PK733_06860"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ10299.1"
                     /translation="MKARRGISIVLCLILFIGTSLSVILVSSANVTLADNNVSDIVSD
                     NVSGSRTKVAETDKIALYYAIDDADNYEIIVEDKRNGYIWSSSFDLEKYSNEKLNNLI
                     ISRMKALFDFKYTYTTNRKFTIVSIDYVSHKPEVSIKLTKNGINMLYEFDKLGISIPL
                     KISINENMLIVNIPVEEIKEEGDYHLTSLDLLPFFGASDNKEQGYMFYPDGSGALFRF
                     KNEPINDISRSGFYIYGTDQVNLSLYEENERKGIETAMLPVYGVKKGENAAFLAVITD
                     GEYDTSINIAPSGFSNIHLNRISPEFTYRRKYGDLRDNPNIPDRIETEILMENREVKY
                     FFLANDEADYSGMANTYRNYLIEEEILSRKINENSKIIPMSLDLFMGINEQRILFDKF
                     IQATTFKQSKTILEEVSQLGVDNVHVKLIGWGAKGYGAYPVFLPANKKLGGVKGLKDL
                     VKYTKEKGYLLYLDANFTDANKGEKGFSIRRDAVFEKNGLVASNKHKDKYLLNPITAL
                     DRFKNQFILKVKEYGIDGLCLERIGNQLYNDYHEKYPVTRKETAENWQEMLEMADAHL
                     GNCAANGGNMYILNQVNRLFDIPTEDSGNMNTSEAIPFYQMVVHGYIPYSSDPGNLFY
                     DYKTQKLKWIEYGCMPYFKLTYSKSDVLKYTDYNVLFSSYYKDWLATAADTYKEFNEH
                     LGDTWSREMIKHEQLQGNVYKVTYKDGTKIYVNYNQRNILVEGYEVKAVDYLVVDKGG
                     NMR"
     gene            17301..18188
                     /locus_tag="PK733_06865"
     CDS             17301..18188
                     /locus_tag="PK733_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ10300.1"
                     /translation="MKQKPYKKPLTMKQKHVLEGYLFIVPWIIGFSIFFALAFFKSLQ
                     LSLSKITKVSGFELEWIGGANYIRAFVWDTTFIPKLIDVLGDIVINTPLTLIFSLFIA
                     MLVNRDIKFRGFFRGTFFLPVLLGTGFVLKQLLGMGVEQQATEVARRIVMPDEILMYL
                     GPNVAGFVDNFMSRITLIFWNSGVQIIIFLAGLQSIPGSLYEASKCDGATVWENFWKI
                     TLPMMSPVILLNAIYTLVDSFASSTNPILEYMLGLKVSISQYEYAAALGMIYFMFVFL
                     LVAVVYGVFKRYTYNSFEK"
     gene            18204..19187
                     /locus_tag="PK733_06870"
     CDS             18204..19187
                     /locus_tag="PK733_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10301.1"
                     /translation="MELNLEFVSLKNRINKWEESNPKLKFLYAKIKNMSIWGKSLFRL
                     ASYLLLFDLAFIFLYPFLYMIITSLKSPIDVMDITVEWIPRSFYFSNYEKAAEMLSYF
                     PILFKTSIVTILATLGHIFSCSMVAYGFARYKFPLKNVLFNLVLLTIIVPIQVLIFSL
                     LIMYSKVGWLNTYLPLIVPTFFGFGLRGGLFIFIFRQFFAGLPYELEEAARIDGCNAF
                     RTYWTIVLPVAGSALLVCIMLSLVWHWNDYFEPTMYILRTDVQLLPSKLPNLYILLEQ
                     MVEQDVEISEYISVEGTVMAGTFLVILPILIVYLFLQKKFMEGIERSGLVG"
     gene            19208..>21355
                     /locus_tag="PK733_06875"
     CDS             19208..>21355
                     /locus_tag="PK733_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10302.1"
                     /translation="MKYGKILKIAAYVLILSLFCIACASCSSNGTQSKNDSSNKSSDT
                     TKGKTDMNMDIVNVKDFGAKDFIEPVECKLDETDTVVAVNGIGNLSVGDNVRIANGVK
                     TGIPGVDLRAEIIAIEGNKITLDRNSYSSITTKLYIDSTDAFKKAFDYANSNDVDVVI
                     PEGNYYVDSQVNRQVFIQTNVKCEGKIYTTNKGRIPLFIISRKEEPIEIRGSSIAGSL
                     EAGTKKIPELGQYAGYDVMFQSSEILMLRNNGVGAFYSKNEANRIKSDGSLAVEMVES
                     YNNKNLLLISLYKQEKELKIDGLNVLCLEKTATDLGKTIIEVNRSNVTFDNLILDNEN
                     LEKEIGQYLGVLINESVNVTLNNSKIRGFMRQGLGYPVRPQVTLYVTLNNTDISYARH
                     AISGMYDNYTTINGGSYEAYAGVIDRHWGHNLTVNDAIIKGEGAAFCCDGGNLTVNNC
                     DITTGYYRLMTRRDCPSFKGNLEIKNTKVIYTGTSNFKLYYSPAITFDHDADLYNPNL
                     IIENLKLELVNYLPVVDIYHIENPVNINYTPQKLPETIWVKDLYITGKDDIKLKNTYF
                     RMLSSTSKYYSEGTPDIYLENIKINSESSIYNYDGLIAYHIYPDNTEITDTHFNLEVK
                     NCGTLSFEIVPAILGDILVENCKIVKYSYYGDLLKDWPNNTVWWQKGTGKTVFKNVKF
                     DRTGLSPSTIYIMNDAEFENCEFTNFGTIDYMNF"
BASE COUNT         7533 a         3085 c         4304 g         6433 t
ORIGIN      
        1 caggcatctg tttattcttg aaacaccgaa tgtctttaaa catccggaaa caggatatat
       61 ggaagaagaa aaaagcaatc taaataaaat agaaaacctt attaaggaaa aattcccaaa
      121 ctccgataca gcaaagatca ataagttccc caacacgtca cagatggaac aaacaataac
      181 aaaaacaatg aattataata gcataataat agtaacctac aaccggacca gtggttatac
      241 cggttcagct gatgcaacaa gacggttcct tgcactgata aatgctgtag cacataaatt
      301 atctgccata ataatatttg gcaacccctt tcctgcaaga gaatacccca ggacacccag
      361 acttattata ggctatgaaa atgggagctg tgaagaagaa gtaattaacg ttttaacagg
      421 tgaaagtatt gccaaaggca aattgccaat agatttggtt agatgaatga tttaggtgta
      481 agtatgcact tataaacatc agtgaaatgt agaaatgtac tgtgtatagc gcacaaaagc
      541 tgtattgttc atggaggtaa atacaatgga ttatattatt gaaccagcaa aaaggatacc
      601 tatagataat gaggttgata tttgcgtgct gggaggaagt tgcacaggac tgtttgcagc
      661 agtaagggct gcaaggcttg gggcaaaagt tgcaatagtg gagaaacaaa attgctttgg
      721 aggggttgca accagcggtt tagttaatat atggcatagt ctatatgata ctttaagaga
      781 aaaacagata attggcggtt tgacctatga aaccatacaa cgattgaata agagagaagc
      841 aattgaatat ataggcaaaa ataaaacagg tccttttgta ttaaatactg aggaattaaa
      901 aattgaatta gatgagcttg caaaatcatc cgacataaaa atatattttc atacacttta
      961 tactgcacca tatatagaag atggtgaatt aaaagctgtg tttatcgaaa acaaatccgg
     1021 ccgtaaagca attaaagcaa aaatattcat tgatgcaaca ggagatgggg atttatgtgc
     1081 atcattaggt gttccatata ctatacctga taaaaaacaa cctcctacta caagtgcaaa
     1141 aatctataga ttacatgaaa agctatcaag aaaagatttt tctaatttaa ttattgaata
     1201 tggaagagag ttcggtttgg aagaagactg gggatgggct gggggaattc ccggtattaa
     1261 agaattaaca tttcatgctg aaactcatgt atttaacgta aattgtgcag atgctgatca
     1321 gttaacatat agtgaaatag aaggtagaag gcaaatacgt gcttacatgg atattgtaag
     1381 aaaatatggg cctgatgaag taaaaccgca tcttgttgca ttagcttcat atataggggt
     1441 aagagaaaca aggcattttt gctgcaagta tagactgaca gaggaagact tgctttatgg
     1501 aagaaaattc aatgatgcag ttgcaaacgg ttcatatcgt gtcgatatac accatgaaga
     1561 taaaaaagga attactctga aaaatcttga tggaagagaa gtatatatca gaagaggaac
     1621 acctgatgta gttaccaggt ggaggaagga tgatgggtat actaattatt accaaattcc
     1681 ctatcggtgt cttgtaccaa atggacattt tgataacttg ttggtttgtg gaagaatgat
     1741 agatgctgat aagggctcgt ttggagctat cagggttatg gtaaatctta accagacagg
     1801 tgaggcggca ggtgtagctg cctatttagc tttaaataat aattcgtctg ttaaagaact
     1861 aaatacagat actataagaa agatgcttaa gcaaggcggt tcaataattt tttaggacat
     1921 aggaatttat aaatttccta agaaattcct agactgattt gttagtgtta gctatctcat
     1981 ggcaaaatta catataagac ttatgaaaaa gtatcccggg ataatgcttt aaaactttta
     2041 aaagagagat gatgaaaatg agtaaagaga ctaaaaggac aggtagtatt atgattaagg
     2101 caaataataa tgtagatttt attgaattac ggccgcaaca tttgctctgt attttatgca
     2161 tgaatggcgg ggcttatctt ccaattatgg agaagcataa tcttaaagaa aaacttgaaa
     2221 aaataaaatc aaacccggat atacacatcc gccttgttac aggttttgac gaaatcggtg
     2281 tacctactga tattttttat gagctgacac cacaggaaag aaagagagat ttgagcatac
     2341 tgcaaaaact tgggcttgta cccaactcaa taagaacagc aaggatatta atacagagaa
     2401 taaatgaaaa tataactaac ttacatgaaa tctgtgtaac gaattgcatg cctacagata
     2461 cttggcgtga ttgttctctt gcttctgcag gatattttga tgagggtaaa aaagatatta
     2521 ttagtaccag agattctaat gaaataataa aagctaaggg agattccgct aatgtggtta
     2581 atgaaacaga acaactattt gtaagagccc accatctttt atgtatcttg tgttctatag
     2641 gagccaatac agataagccc aaaatacaaa ataacactta tgaagtatgg aaaaaaatta
     2701 ttgataaccc tgatataccg gtaacaattg ttgaaggatg cagccaatgc atgatatgca
     2761 aaccttgcaa gaattacaga gccgatagga gtatatgtgt ttctttatct ggactcagag
     2821 acagactaaa agatctgact gtgtttcaga agctgggact caacccggga gatacaattc
     2881 ctgcaaaagt actttataag atgattgctg aaaaaatacc tgatacgttt ggcatatgtc
     2941 aatatgagag agagacttca ccagaatgga gaaattgtat gagtctcgga gtttacgaga
     3001 aaggtaaaaa taagataatg gaactgttat aagaagctgt tatacaaaat tatgaggtat
     3061 tggtactaaa cgtttaaatt tgacagattt agcaatagaa caaaattaat aaatattaca
     3121 atttgtaagg aggaattaca ttgttaaaga aaattgatat tcatgctcat tcaattcgta
     3181 taaaggggat taatttttat aagagtaaaa taacactagc tacaccagat gaagtagcag
     3241 aagtatatgc cagaatcgga gtggaaaaag ggatattgtt acccttgata aatacggaat
     3301 gtggaggcag aatgcaggga aatgaagatg ctattgaaat agtggagcag tatccggatt
     3361 tgttttactg gttttgcaac atagaccccc gcatgggtct gaacagtact aagacagatt
     3421 tatcatttta tctggaatac tacaaagaaa gaggtgcaaa gggtgtaggt gaaattggtg
     3481 taaatatgta tttcgatgat ccgtttatgg aaaacctttt ttatcactgc cagaaaaacc
     3541 agatgccaat cttgtttcat tcgtcaactt gcattggagg tatatatgga cttgctgacg
     3601 aagtggggct tccacgcctt gaaaaggagc ttggcaaatt tcctgatttg gctttcatag
     3661 gtcatgcacc tccattctgg tctcatataa gtaatgactt gacagctgaa aattggggca
     3721 actggtatta tccaaacact aaggtaatac ctgatggaag acttgctaag cttatgagaa
     3781 aatatccaaa cttatatggg gatatttctg caggaagcgg aagtaatgca atgcttcgtg
     3841 atcctgagtt ctcatatgac tttattgagg agtttcagga ccagctgtta tatggaaccg
     3901 atattagtag caataaattt aatttaaaga tatcagaatg gcttgatgaa gcagtacaga
     3961 atggtaaaat tagttataag gcttatgaga aagtatcaag acttaatgca ttaaaattgc
     4021 tcaaagaaat ataaaatttc tgaacctaga atctttcaag taaattctat aacaccgaga
     4081 agattttgta caggaagaag gtgcaggagg catcagtatg aaaaacataa ttagaataaa
     4141 aaataattat cttataagta aagccttggt tttagtaata ttgttactaa cattactatc
     4201 ttcatgtaac tcggggaaaa ttgatgtctc ctataacgaa aaaagtataa atgaaaaaac
     4261 taatgaagca agcgattctg ctactgtaga aacagattcc cataaaaatg ttaccagtat
     4321 aattaatccc attgtagaag aaaagattac actgaaaatg ctatgccagg ctaatcagga
     4381 tctgaacaat gatatgcctg tactacagga attggaaagg ttgactaatg ttcatctgga
     4441 attcgaaata ttacctgcag tttcagcaga acgttgggag aggtttgctg ctgcaatggc
     4501 agccagagaa ccctttgatc ttgttacata tcaatataga aatgacatta acaagtatgg
     4561 aatggaaggt gtgtttgttc ctttaaacaa tttgattaaa gaacatgcac ctaatcttta
     4621 tgaaaaactt aaagatcctc ttgggaatga acctgtgcca tataaaacaa atgtattgca
     4681 ggatataacc gcaccggatg gaaatatata caatgttccg cttatttcca cagcaaatgc
     4741 cataggagca gtatgggcaa tcagaaaaga ttggttggat aaattgggct ttcaatctcc
     4801 tgggacaatt gatgaattat atgaagtcct taaagctttt aaaaagaatg atcctaatgg
     4861 gaatggcctt tcggatgaaa taccattggg aataacagga gaaggaacat tagtttatag
     4921 cttgcttcct ttgataaatg gcttcggtgc tcatattact ctatatttgg ataaaacaga
     4981 tgacactata aaatatggtc ccgttgagag tgtatggaaa gaaggtatgc tgttcttaac
     5041 caaattatat aaagaagaac tgatagatcc tgattggaca acgagttcta aagacggttg
     5101 gatagcccag gtatctacaa atcagttagg tatgatgtat acatggccta ttagtggaat
     5161 tggcgctgca aacattgagt taatgaaaat agataaaaat tatcatttta tagccatacc
     5221 gccatttaaa gcaccaaatg gagaccgctt taaagataca tccactgcag ggaatattgt
     5281 tgtgccaaga acagctatta ctgcaactaa caaatatccg gtagaaacta taaagtatct
     5341 tgatttctta tttactgatg caggtaatag ggttcttaga tatggaattg caggtataca
     5401 ctatgatatg gttaatggga aacctgttgt taaaaaatct cttcttacag atacaacagg
     5461 tcaagcagca aaagatggta ttgatattgc gcctttaccc tatatttcat tatgggaaaa
     5521 aggtgaccaa tttaaagctg tcgacccaaa ttttaatccg gaagaagatg cccattatat
     5581 atacagacag cctggtatgg tagaagctcc actacctgca ttgcaatttt tagatagtga
     5641 attatataaa ggaccaaatg cttttaaagc aataaatagt tatgcataca gcaaatggac
     5701 tccctttata acaggcgaag agtctcttga aaaacatgat gattatgtaa aacaaattaa
     5761 aacactgggt attgacgaag tattaaaaat ttacaattct gcttataaaa gatataaaca
     5821 attaggcaag taataactat tgaaacatgt aaagattatt tacattctat atctattttt
     5881 tactacattt taaaaaagca aaaatagata tagaatgtaa aaatgtgaat cttgttattc
     5941 caatctttaa actatatact aaaaccgcaa taaaaaggtc gcacatttaa gttacacatt
     6001 aaacttatca aaaacagtct ccgttggttg cataatgtat aaaattagtt gcataaaaaa
     6061 tgacactaaa attagattag taaagcatca gctttattaa taaatacttt atttatttaa
     6121 ggaggaatcc atatgaaaaa caagaaattt ctatgtctca cttgtttagg cttaatgctg
     6181 accatgttgt ttacttcctg tgtcgtcaaa gaatctggaa caacacctaa tacaattagt
     6241 cccggtacta aggaagcaga gtctgaaaaa tttgagccgc ttttaaagga aaagattaca
     6301 ttaaaaatgc tatgtcaggc taatcaagct cttagtaacg acatgccggt aattcaggaa
     6361 ctggaaagat taactaatgt tcacctggaa tttgagatat tacctgcagt ttcagcacag
     6421 cgttgggaaa gattcgccgc tgcaatggca gccagagagc cctttgatct tgttatgtat
     6481 caatacaaaa atgaccttgc taaatatgga atggaaggtg tgtttatacc tttaaatgac
     6541 ttaatgaaga aacatgcacc ctatctttat caaaagctta aagatccatt aggagatgca
     6601 gttgtacctt atacaacaaa tgtattgcca gaaataacag caccagatgg aaacatatat
     6661 aatattcctc tcatttctcc tgcaaatgct gtaggagcca tatggggaat tagaaaagat
     6721 tggctggata agttaaacct taaaatgcct gaaacaacgg atgagttgta tgaagttctt
     6781 aaagctttta aagtgaatga tcctaacgga aatggtcttc cagatgaggt tccattaggc
     6841 tgtacaggag gaggtacatt agtttataat ttcctgcctt tgttaaatgg ctttggtgcc
     6901 catatcactt tatatgtaga caaagctgat gataccataa agtacggtcc cattgaaagt
     6961 gtatggaaag atggcatgat gttcctaaat aagctttata aagaagaact aatggatcct
     7021 aattggacaa ctgctactgc aaacacttat aatgcacagg tgtccggaaa tcagctgggc
     7081 ctgatgtatg catggcctgg atccggactt ggcaacgcaa ctaaagaatt acagaaaatt
     7141 gatccaagct atcgatttgt acctatgcct ccgttaaaag ctccaaatgg agaccgtttc
     7201 aaggatacat ccactgcagg gcaaattgtt gtaccaagaa cagctattac tgcaactaac
     7261 aaatatccgg tagaaacaat aaaatattta gaattcatgt ttactgaaga gggtgacaga
     7321 tgtatccgct atggaatcga gggtgtacat tatgatatgg ttaatgggaa acctaaaata
     7381 aaacaatatc ttttagatga taaaaccggt ctgggtggaa aagatggtat agatatggca
     7441 ccattaggtt ttttatcact atgggacagg caagcccaat atgacgctaa caatcctaat
     7501 tatgtcaagg aagaagatcc atactatgtt tacatgcagc ctggtacagt agaagcccca
     7561 atgccttcat taattttcat ggatagcgaa ttggaaaaag ggccagatgc ttttaaagca
     7621 ataaatagct atgcatatag caaatggact ccctttattg taggtgagga acctcttgat
     7681 aagtacgatg attatgtggc acaaattaaa acattgggca ttgatgaagt attaaggatt
     7741 tataataaag cctatggcag atatagacta tcaggtaaat aaaattcaag tactaaaatg
     7801 tagcaaaaat taagaatgca tcaatgactt tactaataat acaaataatt actagcgggc
     7861 actcctcacc ttttacctca atgtagggag gagtgtcccc atatcaaagt tatacaaaat
     7921 aaataaaaga aatgcgaggg agtttctctt gaaacatata tatatctata ggtatattta
     7981 taaacacatt tataagaata tttataagaa cctgtgcttt cttatagctg taattttact
     8041 gataaccctt gtgcctgcag attcacaagc tgaacctgat ttaaatttaa aattgagtga
     8101 gaaattcaag acaaacaaga ctacaaccaa tagtaataat aatagtgata ataatgctga
     8161 atcaggtatt tacagcggtg tactgataga taaggaaaac tcatattttg acattcagga
     8221 ggaatataag gaaaaaggat acagaccggc aagtgatatt gaaatattcc tggatatgca
     8281 atcaatcact tcaaattccg atgaaaatgc tcccatactt ggggaagtag caggaaaaaa
     8341 agatgtcctg gtgtgggaag aagacaagga atattttgaa tggactgcgg aaatacccca
     8401 agatggccta tatgaagtat tagtcattta ttatccgttg ggagggacaa gtaatactat
     8461 acaacgttca ataatgatag atggggaaat cccttttcta gaagcctata atatctattt
     8521 caatagaata tggagagata gggaagaacc caaggtaaat aatgtaggtg atgaagtaag
     8581 gccaagacaa attgaagaga aaatatggca gtcagcaata ttgactgacc atcaaggaat
     8641 gtattcagag cctttcaagt tttattttac taagggagca cataaaatac ggctgatata
     8701 tgttgatgaa gccattgcta ttagtgatat tgttgttcgg acgcctggta gaattccttc
     8761 atatgacgag gttttagatg aatataatca aaaaggatat aaagatgcga gccaaacatt
     8821 tagatttgaa gctgaaagca atgtagtaag gaaaagtgat cctacaatac gaagggaaag
     8881 tgataacgac cctataactt atcctgcctc cgcaacaaac agaaagctta atattatggg
     8941 aggctggcgc tggaaaacag gaaatcagtc catcacgtgg aaatttagtg ttccggaaga
     9001 tggtttatat aaaatcggta tgagaatagg gcaatggtat ggagatgggc tgccggttta
     9061 cagacaagtt gcaattgatg gaaaagtgcc atttgatgag cttaaggaat ataaatttca
     9121 atacaataag aattgggaaa ttaatgtttt gggcgattca aatgaaggcg cgtatctctt
     9181 ttatctggaa gaaggagagc atgagctgac tatgacagtg aagatgggga cattaagagg
     9241 gattattgac tccatttatg aagactcatt actactttct aaagtgataa ggcaaattat
     9301 tatgttaacc ggaagtgaac cggatattaa ctttgaatac gatctggata aaaatatacc
     9361 ggatttactt gaaaatttaa caaaattatc tgttaatatg cagggaaaag cagaattact
     9421 taataaaatt tctactaaaa gccctgcaat ggcaaataac tttttaatga taagcgacca
     9481 gatggaaaaa atggtgaaaa aacctgaaac tataccactt aaacttaatg atttaaataa
     9541 tgcgttaaat aacctgggta tatggatcct tagtctgcaa gatcaccctt tggctattga
     9601 ttattttata gcagggccat ctgatgaaaa gttggaaaat aagaaagcta acattttaca
     9661 aaaattagcg gcaacctggc agaattttat tatttcattt acaaaagatt atgacaatat
     9721 cggtagtgtg tataatgatc aaatagaaac aaatgaggta attgatgtat gggttgccag
     9781 aggaagagaa tgggcggaga taattaagga aatggcagat gagagcttta caccaaacac
     9841 aggtattagt gtaaaaatta atgtcattcc ttctgtagat acattaaatg cacttttgct
     9901 ctcaatttgc tcaggaaaag caccggatgt agcctgcagc gttcaatcaa attatccttt
     9961 cgaatatgct gtaagggatg ctgtggttga tttgacacaa ttcgacgatt ttaagcaggt
    10021 gagcggacat tttcttccca aaataatgat tccgttccaa tacaacggag gagtttatgc
    10081 attaccagag accatgaatt ttaaagcatt attttaccgt aaggatatta tagaagagtt
    10141 aggcattgaa attccagata cctgggatga gttgtaccaa catgtattac cggtattgta
    10201 tcaatatgga atgcaatttc attatccatc caccgatttt gggcctttgc tttttcaaaa
    10261 agggggcgag ttttataagg atgaaggtaa aaaatctgcc cttgattcat ctgaggcata
    10321 tcaagctttc cgcgaattca cagaattatt tacgaattat ggtataccca tatctgcaaa
    10381 cttttttaat cgtataagac agggggaaat gcctatggga ataggtgatt attatactta
    10441 tgtgcagctt tccgtagcag ctcctgaatt atctggaaga tgggatattg ctccgttgcc
    10501 cggacatata acagaagatg gtgaaatcaa ccgttttacc gatgggatag cagaaagtgc
    10561 tgatattatc atgaaccagt cagataagaa gaaagagtcc tgggaatttt tgaaatggtg
    10621 gacaagtgat gagacacaag ctaagtttgg aagtgaattg gaagctttaa tgggagccca
    10681 agcacgctgg aatacagcta atactgccgc attcacaaat ctgccatgga acaagcagca
    10741 tttaaaagtg attcaaaagc agtgggagtg ggctaccgaa gcaccttcgg ttttaggagg
    10801 gtattttacg caacgtcata caaaaaatgc ctggaataga gtagtgattg gtggaatgaa
    10861 tgtaagagat tccatggaac ttgctgtaaa agatataaat agggaattaa gaaataaaca
    10921 ggtggaatac ggatttattg atgaatagtg gagatggtcc ttcattttta acaaaaataa
    10981 aatagggtaa ttgtagaaag tgaaggaccg tccccataaa gaaaggagta agcagctgtg
    11041 aataatgtag tagaaacaat taatgaacaa aaggtgacaa taaaggcaag tcttggtttc
    11101 ggaaaaaagt ttaaggcttt ttggggcaaa tatggaatag ggtatttatt tttatctcca
    11161 tatttaatat tattcactat atttatcatc atccctgtag ttgtagcaat cggaacaagt
    11221 tttacaaact ataatatgct gcagagacca aagtggcttg gaatatcaaa ctaccgcctt
    11281 ctatttacag acgacgatat cttcctgatt gctttgcaaa atacagctat ttttgcttta
    11341 ataagcgggc ctgccggcta tattatgtca ttttttgcgg catgggttat aaaccagtta
    11401 aagtttaaaa aggtctttgc attggctttt tatgcaccgt ctataacaag cggagtagct
    11461 atgacggtag tatggatgtg gttctttgcc agtgaccgct atggaatgat aaataatgtg
    11521 cttataaatt taggaattat taatgagcct gtgctgtgga atcttgatcc agctacaatc
    11581 cttcctgtag ttatttttat ctccgtatgg atgagtatgg gaacaggatt tcttgtattt
    11641 cttgcaggat tgcaaagtat atcaagagaa ttgtatgaag ctgcatcaat agatggtata
    11701 aaaaataagg tacaggaatt gtggatgatt accctcccac taatgaaacc tcagcttctt
    11761 tttggtgcga tcaattctat tgtagcttct ataggtgtat ttgatataac taagtcagta
    11821 gcaggaatgc caagcccgaa ttatgcggca cacacaattg ttgcccatct ttatgattat
    11881 gcttttttaa ggtttcagat ggggtattcc tcggcaattg ctgttgttct attttcaatt
    11941 acatttattc tgggcagagt atgtatgaga atattttcat caaaagatat atattagaaa
    12001 ggtgtgaaat ataataatgg caaaactata catagcaaac aaaagaagta aaaaaaagaa
    12061 gcttaagcgt atgaccaaag gatgggcctt tgtatatata tttatgattg ctctggtagc
    12121 ttttacatca atgcctttaa tttacgtgat tagtacggct tttaaaccaa tggatgaatt
    12181 atttgttttt cctccgagat ttttcgtccg caggcctaca atccagaatt tcacagattt
    12241 gctgatttca ttagggagcg ctacagtgcc atttacccgt tatttattca acagtctttt
    12301 tactgttact gtaacagtct ttattacagt tgtaatatca agtttagggg cttttgccct
    12361 agtaaaatac aaattaccgg gaggaaaatt gatcttttct ataatactag cagcactaat
    12421 gttttcacat catgtaactc aaataccaaa ctatatggtg gtaaatagcc tgggaataat
    12481 aaatacttat gtggtactaa ttattccaaa gcttgcagtg gcatataatt tctttctgat
    12541 gaagcaattc attgagcaat ttcccaacga attgcttgaa tcgggaagaa tggacggtgc
    12601 aaaggaatgg gggctattct ggaaagttgt tatgccttcg ttaaagcctg catggtcaac
    12661 cctggtagtt ttgtcttttg taagtaattg gaacgactat tttacaccat tagtatttac
    12721 cactagtgaa gccatgaaaa cattaccata tgcattaaat accatatcaa gtggaaaagc
    12781 tgtagcccgt gtaggggcaa cttctgctgc agtgtttata ataaccgtgc ctaccataat
    12841 aatatttaca gccatgcaat cccaggttat ggagactatg gtatattcag gaataaaggc
    12901 ataggagatg agaataatga gggcttttgg agaaatggat actaccaact ggttaagaag
    12961 gttaatcaga ataagaagaa aaagaggaat tagaataata agagcggtta acagattgtt
    13021 attgttaata gtaatattta cactctgttt ctcacagtca gcaaaagccg agaggcaaca
    13081 atatatttgg gaaaatgact ggagaattcc tatccctaca tcatacagaa acgtgaaagt
    13141 aataacatac cttggcgaaa aaagtggttt tttgaatggt gcagaggatt tatttataga
    13201 taaaaatgac tgtatgtatg ttgcagatac tttaaacaac cgtgttctaa aactgacctt
    13261 agagggtgaa acattggaag tatttacagg accatctgaa aaacctttaa aaaatcctaa
    13321 gggaatatat gtagatgatg atggtcacat gtttatagct gatacaggaa atcttcgtat
    13381 tattcatctt tcaaggaaag gtgaatttat tgaagagttt ataaagcctg aatcaaacct
    13441 gttaagtgag gactttactt ttgacccttc taaagtatac ataaattcaa caggatatat
    13501 atttattatc aaaagccagg cttttttcag tatgggtgct aataatgagt ttcaaggtta
    13561 tataggttct acaaaagtag gatttgattt ccagagaata ttaattaata tgtttgcatc
    13621 agattttcaa aaaagccagg tcataaaaaa attacccgat tcctattcga attttgtaat
    13681 agatgattat ggcatgattt atgcgacagt tataaatgct aaaagaggtc agataaggaa
    13741 aattacatca gcaggtaaga acatatatcc tggaaaattc tatggagaaa ttacgcgctc
    13801 aggatggaga tccaaaatac caaattttgt agatatagct gttgacaaaa atggtatcgt
    13861 tagtgtagta gatcaagcat cgggcaagat ttatcaatat gatcaggaag gtaatatgct
    13921 tgcagctttt ggaggccttg gaaaccagaa aggtacattc aaaatccctt caagtatagt
    13981 tttagacagt cagggaaggc tttatgtctt agatcagaaa gcaaacaata tacagatttt
    14041 tgaacctact aattttataa atttggtaca tgaagcatca aggctttaca atgatggaag
    14101 atatgcagaa tcccgggatg tgtgggagaa agttcttcaa attgatggga aatatgctct
    14161 tgctcacaga ggaatcgcta aagctcttat taaagaagaa aaatggatag aatctatgga
    14221 aacatacaaa ctggctaatg accaggaagg atactcagaa gcatttattc agtataggca
    14281 ttccatatac agaagatatt ttggttggat tattctttta atagcagtag taattgttgt
    14341 attgttttat atgcttaaat atttaaaaaa attatcagaa tcagtattgc gaaagcatat
    14401 gggctggtaa taaggaggaa ttagaatgaa tgtttttgaa atgtcaaatg ctatactatt
    14461 ccaccccgtt gacacatttg aatacataaa atcaaggcag aaatcaatca gtataatatc
    14521 atgcatactt ttgttgtttg cagtggtagt tgtccgtata gcatctatct tcattgtaca
    14581 ttatccaatt gcatccatac agcccaggga tgcaaatatt tggctggaaa tagtaaaatt
    14641 gcttcttccg gttcttacat gggtaatttc atgttatgcc attacagcta tttcagatgg
    14701 ggaaactttg ttaagagaga tattttctgc agcagtattc tccatgatac cctatattgt
    14761 atgtacaata ccactggcta taattaccag ggtaatggga agagatcagc ttcagcttta
    14821 taatacactt aataatataa tattaatctg ggtattaata attatttttt taagcgtaag
    14881 aacactaaat gattatacaa ttgttcaaac tgtaaaagta tgtattataa gttttattac
    14941 aatgttgctt atatgggctg ttctaacact tatatttgcc cttacaagcc aattaattca
    15001 atttttatgc aatatagtca gagaactaag aatgatagtt attgaatgaa gagaatgagg
    15061 agtgaagagg caatgaaagc tagaaggggt atatcaattg tattatgcct gattttattt
    15121 atcggaacgt cattaagtgt gatattggtt agctctgcaa atgtgacact ggcggataat
    15181 aatgtttctg acatcgtttc tgacaatgtt tccggcagcc gtacgaaagt agctgaaacg
    15241 gataaaattg ccctttatta tgctatagat gacgcagata actatgaaat aattgttgaa
    15301 gataaacgta atggatatat atggagttct tcttttgatc tcgaaaaata tagcaatgaa
    15361 aagttaaaca atctaattat cagcagaatg aaagcattat ttgattttaa atatacttat
    15421 actacaaaca ggaaatttac aattgtttct attgactatg tcagccataa gcccgaagtg
    15481 agtattaaat taacgaaaaa tggcataaac atgctttacg aattcgataa actaggtata
    15541 tctattcctt tgaagatttc tataaatgaa aatatgctta tagtgaatat accagttgaa
    15601 gagattaagg aagagggaga ctatcatctt acaagccttg atttgctgcc tttttttgga
    15661 gcaagtgata acaaggagca agggtatatg ttttaccctg atggaagtgg agctttgttc
    15721 aggtttaaga atgaaccaat aaatgatata agccgctccg gtttttatat ttatggaact
    15781 gatcaggtta atttatcttt atatgaagaa aatgagagaa aaggcattga aactgcaatg
    15841 cttcctgtat atggcgttaa gaaaggggaa aatgcagcat tcctggcggt aattacagat
    15901 ggtgaatacg atacatccat aaatattgcg ccttcaggat tttctaatat tcatcttaat
    15961 agaataagtc ctgaatttac ttatagaagg aaatatggtg acttgaggga taatcccaat
    16021 attcctgata gaattgagac agaaatactt atggaaaaca gagaagtgaa atatttcttc
    16081 cttgcaaatg atgaagccga ttacagtggt atggcaaata catataggaa ttatctgatt
    16141 gaggaagaga ttctttcaag aaaaataaat gaaaacagta aaatcattcc tatgagtctt
    16201 gacttattta tggggataaa cgagcaaaga attttatttg acaagtttat acaagctact
    16261 acttttaaac agagtaaaac tatattggag gaagtttcac agctgggtgt tgataatgtg
    16321 catgttaaat taatcggatg gggagctaaa ggatatggag cttacccggt atttcttcct
    16381 gccaataaga agcttggagg agtaaaaggg ctgaaagatt tggtgaaata tacaaaagag
    16441 aagggatacc tattatacct tgatgccaat tttactgatg ccaacaaggg tgagaaaggt
    16501 ttttcaatcc gaagagatgc agtatttgag aaaaatggtt tggttgcttc aaataagcat
    16561 aaagataaat acttgctaaa tccaattaca gcacttgata gatttaaaaa tcagtttatt
    16621 ttaaaagtaa aagagtatgg gattgatgga ttgtgtttag aaagaattgg aaatcaacta
    16681 tataatgatt atcatgaaaa atatccggta acaaggaaag aaacagctga aaactggcag
    16741 gaaatgcttg aaatggcaga tgctcatctt ggtaattgtg cagcaaatgg tgggaatatg
    16801 tatatattaa atcaggtaaa tcggttattt gatattccta ccgaagactc gggaaatatg
    16861 aataccagtg aagcaatacc cttttaccag atggtagtgc atgggtatat accttattct
    16921 tctgatccgg gcaatttatt ttatgattat aaaacacaaa aactaaaatg gattgaatat
    16981 ggatgtatgc cttattttaa attaacttac agtaaatcgg atgttctgaa atatacagac
    17041 tataatgttt tattttcctc ttattataaa gattggcttg ctactgcagc tgatacatat
    17101 aaagagttta atgaacattt gggggatact tggtccagag aaatgattaa acatgagcaa
    17161 ctacaaggaa atgtatataa agttacatat aaagacggaa caaaaattta tgtaaattat
    17221 aaccagagaa acatattagt cgaaggttat gaagtaaagg cagtagatta tcttgtagtg
    17281 gataagggag gtaatatgag atgaagcaga agccttataa aaagccatta accatgaaac
    17341 aaaaacatgt actagagggt tatttattta tagtgccatg gattattggg ttttctatat
    17401 tttttgcatt agcttttttt aaatcgctgc agctaagttt aagtaaaata actaaagtct
    17461 caggatttga actggagtgg ataggtggag cgaactatat aagggctttt gtgtgggata
    17521 ccacatttat tcccaagctt attgatgttt taggtgatat agtcataaat acacccttga
    17581 cactgatatt ttctcttttt attgcgatgc tggttaacag agatattaaa ttcagaggtt
    17641 tctttagagg tacatttttc cttccggttt tactaggtac cgggtttgtt ttaaaacaac
    17701 tgttgggtat gggtgtagag caacaggcaa cggaggttgc aagaaggatt gtaatgccgg
    17761 atgaaatttt aatgtatctt ggccctaatg tagcaggatt tgtagataat ttcatgagtc
    17821 ggattacttt aatattttgg aattcagggg tgcaaataat catatttttg gctgggctgc
    17881 aaagcatacc aggttcatta tatgaggcat caaagtgtga tggagcaaca gtttgggaaa
    17941 atttctggaa aattacactt ccaatgatgt caccagtcat tttattaaat gcaatttata
    18001 ctctggtgga ttcgtttgca tcttcaacta atcctatttt agagtacatg ttggggttga
    18061 aggtaagtat aagtcaatat gagtatgcag cagcacttgg aatgatttac tttatgtttg
    18121 tatttttact ggtagctgtc gtatatggag tatttaaaag atatacatat aattcttttg
    18181 aaaaatagga ggtgcagata aatatggaat tgaatctgga atttgtttca ttaaagaata
    18241 gaatcaacaa gtgggaggaa tcaaacccta aactaaaatt tctttatgct aaaataaaga
    18301 atatgtctat ctggggtaaa agtctgttca gactggcatc gtatttgctg ctatttgacc
    18361 ttgctttcat atttttatac ccatttcttt acatgattat tacatcatta aaatcaccaa
    18421 ttgatgtaat ggatattaca gtagagtgga taccaaggtc tttttatttt agcaactatg
    18481 agaaagctgc agaaatgttg agttattttc caattctttt taaaacaagc attgtaacta
    18541 tattagcaac attaggtcac atattttctt gttctatggt ggcatatgga tttgccagat
    18601 acaaatttcc tttaaaaaat gttttattta atctggtttt gttaactatt attgtgccaa
    18661 tacaggtact tatattttca ttgcttatta tgtattccaa agtagggtgg cttaacacat
    18721 atttgccgct tattgttccg actttctttg gtttcgggct gcgcggcgga ttatttatat
    18781 ttattttccg gcaatttttt gcaggactgc catatgaact ggaagaggct gcgcgaatag
    18841 atggctgcaa tgctttcaga acatattgga ctattgtatt gcctgtagcc ggaagcgcgt
    18901 tgctggtatg tataatgtta tcattggtat ggcattggaa tgattatttt gaacctacaa
    18961 tgtatatttt gagaacagat gtacaacttt tgccatctaa attaccaaac ttatatattc
    19021 tgttggaaca aatggtggaa caggatgtgg aaatatcaga atatatatca gttgaaggta
    19081 ctgtaatggc aggaaccttt ctggttattc tgcccatact tattgtttac ttattcttac
    19141 agaaaaagtt tatggaaggt atcgaacgca gcggattagt tggttaaata attaaggagg
    19201 atttttcatg aaatatggaa aaattttaaa aattgcagct tatgtactca ttttaagttt
    19261 attctgtatt gcctgtgctt catgttcctc aaatggaaca cagagtaaaa atgacagtag
    19321 taataaaagt tctgatacaa caaagggaaa aacagatatg aatatggaca tagtgaatgt
    19381 aaaggatttc ggagctaagg actttattga accagtagaa tgcaagcttg atgaaactga
    19441 tacggttgtt gcggttaatg gtattggtaa tttaagtgta ggggataatg tgagaattgc
    19501 caatggagta aaaaccggaa ttcctggtgt ggatttgagg gctgaaatta ttgctattga
    19561 aggtaataaa attactcttg acaggaatag ttacagttca ataactacaa aattatatat
    19621 agatagtact gatgctttta aaaaagcttt cgattatgca aactcaaatg atgttgatgt
    19681 agtaatacct gaagggaatt attatgtaga tagtcaggtt aatagacaag tattcatcca
    19741 aacaaatgta aaatgtgaag ggaagatata tacaacgaac aaagggagaa ttcctttatt
    19801 cattatttca cgcaaggaag aacctataga aatcagagga agcagtattg ccggaagcct
    19861 cgaggcaggt acaaagaaaa tacctgaatt ggggcaatat gcaggttatg atgtaatgtt
    19921 tcaatcaagt gaaatcctta tgttgagaaa taatggtgtt ggagcatttt attcgaaaaa
    19981 tgaagctaac aggataaaaa gtgatggttc tcttgcagta gaaatggttg aaagctataa
    20041 taataaaaat ttactactga taagcctata taaacaagaa aaggaattga aaattgatgg
    20101 ccttaatgtc ctttgccttg aaaaaactgc aacagatctt ggcaagacaa ttatagaagt
    20161 taatagaagc aatgtaacat ttgataatct tatattagat aatgaaaatc tggaaaagga
    20221 aattggccag tatttgggtg tattaataaa tgaatctgta aatgttactt taaacaattc
    20281 aaaaataaga ggctttatgc gtcagggcct tggttatccg gtacgcccgc aagttactct
    20341 ttatgtaact ttaaataata ctgacattag ctatgcacgt catgcaattt ccggaatgta
    20401 tgacaactac acaacaatca acgggggaag ttatgaagcc tatgcaggag ttattgacag
    20461 gcactgggga cataatctta cagtaaatga cgccattata aagggagaag gagcagcttt
    20521 ttgttgtgat ggaggaaatt tgacagtaaa taactgcgat ataacaacag gttattacag
    20581 gctgatgacc agacgagatt gcccttcttt taagggaaat ttagaaataa agaatacaaa
    20641 agtgatatat acaggcacat caaactttaa gttatactat tcgcctgcaa taacatttga
    20701 tcatgatgct gatttatata accccaactt aattattgag aacttaaagt tggaattggt
    20761 aaattattta cctgtagtag atatatacca tatagagaat ccggtgaata taaactatac
    20821 acctcagaaa ttaccggaaa ccatatgggt aaaggattta tatataacag gaaaagatga
    20881 tattaaatta aagaatacat atttcagaat gttgtcctcc acaagtaagt attactcgga
    20941 aggaaccccg gacatttatc tggaaaatat taaaattaac agtgaaagca gtatctataa
    21001 ttatgatggc ctaattgcat atcatatata tccggataat actgaaatta cggatacaca
    21061 tttcaatctt gaagtaaaaa actgcggaac actatcattt gaaattgtac ctgcaatatt
    21121 gggagatata ctcgtagaaa attgtaaaat agtaaaatac agttattacg gagacttatt
    21181 aaaagattgg cctaataata ctgtatggtg gcaaaagggt acgggtaaaa cagtatttaa
    21241 aaatgtaaaa tttgacagaa ccgggctttc accttctaca atatatatta tgaacgatgc
    21301 tgaatttgaa aactgtgaat ttacaaactt cggaaccata gactatatga atttc
//
LOCUS       DAOSDD010000055        21096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1083_length_21096_cov_4.994202, whole genome shotgun sequence.
ACCESSION   DAOSDD010000055 DAOSDD010000000
VERSION     DAOSDD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21096
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_21096_cov_4.994202"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="dnaN"
                     /locus_tag="PK733_06880"
     CDS             complement(<1..677)
                     /gene="dnaN"
                     /locus_tag="PK733_06880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ10303.1"
                     /translation="MNIKINRDTLLKPLSAVSGIVERRHTLPILSNLLLEARQNSIVL
                     TATDLEMQISLSIDSDAGGELSTTVSAKKLLDICRALPENAEINMATTDSRMQVKAGK
                     SRFNLQTLPASDYPVMTKAAGAEPSVVTISQGVLKRLLKQVEFAMAQQDIRYYLNGLL
                     FEVNANRLNLVGTDGHRLSFTSAELNRNYEKQELILPRKTVIELIKLLDESDEEVTVE
                     IVPGQVN"
     gene            complement(839..915)
                     /locus_tag="PK733_06885"
     tRNA            complement(839..915)
                     /locus_tag="PK733_06885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(879..881),aa:Arg,seq:tct)
     gene            complement(920..993)
                     /locus_tag="PK733_06890"
     tRNA            complement(920..993)
                     /locus_tag="PK733_06890"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(959..961),aa:Gly,seq:tcc)
     gene            complement(1207..2955)
                     /locus_tag="PK733_06895"
     CDS             complement(1207..2955)
                     /locus_tag="PK733_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10304.1"
                     /translation="MAIKKGLLIGLIIAAVILVILAAGIGTVLSIHNSTYDCSFVYVQ
                     PEDLVKEIATKHTYYESDSKTVEIELNQDLINSLIKDNFENLDIALPDKFSINEILFN
                     TKDQRLYINGKYGSLNVPLSAKLDINLTEDGIDITASQLNLGNKKAPGLITNQIPADA
                     LSYSLKYADFDLPDIFKVKDVQFGSGIVKAYIELLPDKIKEMAMDYRNQALDQIDTFK
                     AGQSEFISNFITRILDSTALLSDVKVNEFVDFVLNHGELLNSAIQFALVDDLSDYSEQ
                     FNIAQKAIADWAAPLEAVKYYGSIEETKDKILYDQKLKDLVSWFIPSEKINEYVGTVE
                     EYYGMYEEILDMKDELESTFASLSFSTDSIEETVASLQNKIFRNSRLINLVSDYIPAG
                     TFGDIADTIDEYVGLYNEYMGLLDDTMNNVALTAGKIDVESINEITNTIMDYANQAGD
                     VRELIVDALSSVDTQLVKDLVHFVEFGGKFGQDFIATIDPDNYSIFREYVDNLSEIQA
                     DTIDFYKNFDTSVFVNNATTIKEIGDFSTEVVTLLRNKDVENAIDVLMNKNFNDYQFE
                     VPEFSDYEMQFEIVVE"
     gene            complement(3533..5194)
                     /locus_tag="PK733_06900"
     CDS             complement(3533..5194)
                     /locus_tag="PK733_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ10305.1"
                     /translation="MLKKLNFISQNDFTNHEEFTENFKINVPENFNFAYDIVDEIASG
                     NPDKLAIVWCNDSGEEAFFTFGQLKEYSNKAANFFKSLGIKKGDSVMLILKRRYEFWF
                     CLLALHKIGAIAIPATHLLTPKDIVYRNNAASIKMIITVAEDEVIGHIENSLEDSPTV
                     KHKVLADGNRKGWFNLHEEINKSSSDFTRPVGEEATTNNDISLLYFTSGTTGMPKMVQ
                     HNFIYPLGHIVTAKYWHDVSDGGLHLTVADTGWAKAVWGKIYGQWICGSAVFVYDYEK
                     FVPKDLLDVIVKYGVTTFCAPPTIYRFLIKEDLTKFDLSKLKHCSIAGEPLNPEVYNQ
                     FLKATGLKLMEGYGQTELTLAIGTFPWMEPKPGSMGKPAPGYDIDLIDEEGNSCDVGE
                     EGQIVVRTDKRIPVGIFGGYYRDPKLTLKVWHDNIYYTGDIAWKDEDGYFWFVGRADD
                     VIKSSGYRIGPFEVESALLEHPAVLECAITAVPDPIRGQVVKATVVLTKNYKPGNDLV
                     AELQDHVKKVTAPYKYPRIIEFVDELPKTISGKIRRVQIREQDKK"
     gene            complement(5224..5811)
                     /locus_tag="PK733_06905"
     CDS             complement(5224..5811)
                     /locus_tag="PK733_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ10306.1"
                     /translation="MSNQIKQIAVRIKELREIYSISLETLAEELGIPKGRYEAYESGE
                     VDIPVSFLYKIAGRFNVELTAILTGDNPKLQTYSLVRKGKGVSVERRKAYKYHSLAYN
                     FLNKKIEPFLVTVEPDSDNSDNSGNSDNNGDAGYNSHPGQEFNYVLEGEMQITIDGHT
                     LILKEGDSLFFDSGIKHGMKALGGRPAKFLAIITQ"
     gene            6167..10012
                     /locus_tag="PK733_06910"
     CDS             6167..10012
                     /locus_tag="PK733_06910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOJ10307.1"
                     /translation="MVRRIFVETKNEYDVESQQLYEEITGFLGIENLKSVKVFSKYDI
                     QGITDQQYLEVRNRVLSEPPINNVYEENTGFEEHVMLEKEIRIIAMEYLPGQYDQKAD
                     ITSQCIQIIIGGPRPIVKTARIIVLKGKISDVEFSRIKNYYINPVESKETSLEKLSKL
                     DSDIKEPDDIKILEGFINVSDSEIEEYGNKIGFAMPGEDLLFCRDYFKNQEKRDPTMT
                     EIKVLDTYWSDHCRHTTFMTRIQDIEFDKGPYYEVINRAFQEYLKSREYVYSDKPKDI
                     CLMDLATINMKEQRKRGLLEDLEVSEEVNASSITVSVDVEGMTEEWLVMFKNETHNHP
                     TEIEPFGGAATCLGGGIRDPLSGRAYVYQAMRITGSGDPRTKIEDTLPGKLPQRKITT
                     TAAAGYSSYGNQIGISSGHLSEIYDEGFVAKRMELGAVIGAAPKRNVVRENPCTGDVI
                     ILLGGRTGRDGCGGATGSSKEHTEESLLTSGAEVQKGNPLIERNILRMFRKPEVSRMI
                     KRCNDFGAGGVSVAIGELADGLDINLDKVPVKYAGLNGTELAISESQERMAVMVSKEN
                     EARFIEMAAEENLEATTVAVVTEEPRLKIRWRGKTIVDLSREFLNSSGIRKNTNVKVL
                     SPVNENNYFFSLPYEIDKSLEAGDIEKAWMSNLKRLNICSQKGLMERFDSTVGGDTVL
                     MPMGGKYQLSPAEGMVARIPVLEGETSTATVMTYGYNPSIGNWSPFHGALYSIVEAVA
                     KVAAIGGDYKKIRLSLQEYFERLGSDPEKWGKPFVALLGAYYAQKNLGLPSIGGKDSM
                     SGTFENIHVPPTVVAFAVNTLNINSVISTEFKKAGSKVICVPLRQDKNNIPDFEGMGK
                     NYAQIHELIKTGKVLASSTVKEGGLAAAVSKMCFGNKIGFALSHVKSPQNLFAPDYGS
                     IVLELDNTGDLTQMEQNLQGLDYYELGTTIDEQNIIINNVVISLDKAISSWELVLNDI
                     FPVKTEKVETCAHSIYDSISENKSTSELKAGNFTGRAKLGKTAKPLIFIPVFHGTSGE
                     YEAAQAFEKAGGTVDTFVFRSLTGEHIKQSIDIIEEKINNSQILMLPGGLSGDLQLGG
                     SGRYMAAVLGNPYIKDAINRLLNERHGLILGLGIGFQALLKLGLLPYGEFREMNESSP
                     TLAVNINGNFVSRMVWTKVTSTMSPWFANTKAGDIHLVPIACGEGRFVAGTSDIELLA
                     KNGQIASTYVTNCGSPACDTGSNPTGSMNAIDGITSPDGRILGMVTHPERIDTNTALN
                     VPGNKEQKIFEAGVKYFGW"
     gene            complement(10508..11926)
                     /locus_tag="PK733_06915"
     CDS             complement(10508..11926)
                     /locus_tag="PK733_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ10308.1"
                     /translation="MNEKRPNLIFVFADQWRRQAVGFMNEDPVVTPNMDKFADESLIF
                     ENAISTFPLCSPHRASLMTGRYPLSTGMFTNCKPGVDVALGTHEICISDVLKKQGYAT
                     GYIGKWHLDSSERNFSENPESGAKGWDAYTPPGPKRHGFDFWYSYGANDEHMAPHYWQ
                     DSNKAIHINQWSPEHETDVAIKYINDHTKKYEKNPFALFISWNPPHSPYHMLPEKYKE
                     LYKDKDISFRPNVKAEGLYTHTYEKIGEGFETLTRFTKDYYAAVSGLDENFGRLLKVL
                     KEQGIEDDTIVVLSSDHGDMMGSHGLMAKHVWLEESIGIPFVIRWPSRILPGREKTLL
                     GSADVMPTLLQLMNIPVPASVEGTDLSPVIRKEKTQPVTSAFICACPGRDVFLKELSA
                     KGIDPKTLGWRGVRTYRYTYVVSKGYFPGEKTKRLLYDNETDPYQLNPLCFETAAEND
                     LAFQMELELKEWLSKLDDPFSI"
     gene            complement(12144..13295)
                     /locus_tag="PK733_06920"
     CDS             complement(12144..13295)
                     /locus_tag="PK733_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOJ10309.1"
                     /translation="MTGSALAKNKIDKIELFRYDSDVERHFSFGTWKSRQHAFIEITS
                     GDYCGWGENVMSVNNKNISLSEWGQYFHELNGLSILEAIQYIERKLTDWGSRRCEMAD
                     MALLDLAGHIIGEPAGKLLELNWDEVKNSVPGVYVILHDDPTQVKELAKKAINEKLTT
                     HVKLKLFGKIELDIELVRAIRDVLGEEPYIIGDVNCGYREKLADEGMHDIANQLIKLH
                     ANGLNACEDPAMLTNAQWVELQNMTGGLDLIPDYPLRLAVTAIKTILPGMGRIYNIHP
                     GCTGSIHGAVKLGKKIKEIGGKLMIGDDSLLGPANTAWQQIAIGLGADWVEALVKPGE
                     SDGFLNSVKAQATVRMDGSAITVPKLLPGFGLEIDEDLLRHASWSNFVF"
     gene            complement(13273..14685)
                     /locus_tag="PK733_06925"
     CDS             complement(13273..14685)
                     /locus_tag="PK733_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ10310.1"
                     /translation="MNNKMDKPDILFIMTDHQRRDSIGMVQDNGEVSPNLNRLAEQSA
                     VFTRAYNTCPLCVPARTALATGKYPTKNGVVFNDVKGLRAGNHKTIHQYLEEAGYTVG
                     HVGVNHIQVKPSLKDRMHFDMWIDDNDYAEYVKELDINIRRTEYFEKQVMEFQDDKYV
                     EKKYSNAMTSIWPHPEKYFKDNYFSGKAIEFIQKEHENPFALFVYFWAPHPPLTVPEP
                     YASMFNPDNLQLPGNIGIAAKGEPANRRRGIAAQLAEGLTLEQWRKVWAAHLGLLNLV
                     DAAIGRIFQELEKNGKMDSTLIVFTSDHGDHLGQHNMYQKMEMYEQAINVPFIVKSPN
                     FLTERTGINRHIIDTPVSHLDILPTILDMTGLSIPCDLDGISLADPITRGVAPEERNL
                     FCQYSGNPTVGDIRRAVISRRYKYVYDLRDSAELYDLEKDPLEMDNLAGKASYAEIIS
                     KMHYLCMDWGRSHNDWISFS"
     gene            complement(14904..16535)
                     /locus_tag="PK733_06930"
     CDS             complement(14904..16535)
                     /locus_tag="PK733_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10311.1"
                     /translation="MKFKKTCLFLLAAILVIQAFTGCVKTEFQETKATIPQNNDTDTN
                     LEGNLYKSGIPILKEKENFTVMIRKNALSKNSYPEKLPVQEVEKETNISITWQEIAPS
                     GWNEKVNLSFASKSLPDAIVSGISTSMLMQNIDSLVPIGDYVYKYAPSIKKLWNERPD
                     IKTALTTPDGKIYSFNTSEESAWGVTNSLLYINKTWLDALGMQVPRTTSEFYMVLKAF
                     KDNDMNKNGKNDEIPFSFCQQDAQYSLNSFFGTFGVLDNTQHLIVENGKVIFPSIKNE
                     FRDALVWFNSLVQAGLMDVEGFSQALQQLQAKGKNKELLLGSFLSYNADTATGDNMNH
                     YIPIEPIDGGAGKVLWNKEGQKGGNITGFVVTKSCKNPIAMIRWHDYINSSWENKMRW
                     SFGPEGEAWKFNENGLWIQNNDKLPQGASWGEYRHTVAPASQANWFMTAEEGLYKRLL
                     APRTKIRLDAITDLYQKYFPAETLQSNFENIETANEKNLLLVEIDAYLANFVSNSILK
                     GVTDAQWNEHLKTCKSLNVDRYVQLWQEYYDNQKK"
     gene            complement(16649..17572)
                     /locus_tag="PK733_06935"
     CDS             complement(16649..17572)
                     /locus_tag="PK733_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10312.1"
                     /translation="MKRKTAELNLLKHSSDGIFDFAVNLLLILILIITVYPLYFIVIA
                     SLSDPDAVNTGSMLLYPIGGKDRLLNFEGYIRVFMDNRIWRGYYNTIIYTFFGTLLGV
                     TATILAGYSLSRKDLVGKGPITGIFVFTMYFGGGLIPTYMVVDKLHLINTPLVMIILG
                     SISVYNIIIAKSFFQNTMSVELQEAAFIDGCGNGIFFLKIVVPLSKSIISVLTLYYAV
                     GHWNSFFSALIYLNNEKLYPLQLILRDILIASQMLVSDTTDIESIGDAQRIAESLKYA
                     VIIVSTLPVLIMYPFLQRYFIKGVMIGSVKG"
     gene            complement(17584..18516)
                     /locus_tag="PK733_06940"
     CDS             complement(17584..18516)
                     /locus_tag="PK733_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10313.1"
                     /translation="MKTYGKHEFYRSKWKMVSRNYMLYIFLLPALIYIIIFAYWPMYG
                     IQIAFKNFSSTKGIWNSPWIGFKHFTTFFNSYRFWNLIKNTLTINLYQLVAGFPVPIL
                     LALILNYSTMKKLSKFAQTATYAPHFISTVVMVGMLILFLSPGNGVVNKLLSYMNLKP
                     VFFMGEEVYFKHIFVWSGVWQNAGWNSIIYIAVLTNVSPELHEAAVIDGASKIKRIWH
                     IDVPALLPTAIILLIMRMGNIMNVGFEKALLMQNNLNLNASEVISTYTYKVGLINAQY
                     SYSTAIGLFNNVINFTLLVLVNRFARSISNTSLW"
     gene            18889..>21096
                     /locus_tag="PK733_06945"
     CDS             18889..>21096
                     /locus_tag="PK733_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10314.1"
                     /translation="MKKNSILFKYLISYLLVLFIPLFILSAIIKYYFIKTYSESVLKQ
                     KAMQIQQIKSSIDIQLNQLHNFAIQSSSKNIFSRYNIENNPSSFNDIMKEIRYFIHIN
                     ANLQEVIFHLKDSDIYYMSSGTSKNDYIFTNIMNYEEISKDNSLAFFSNLDAPIWLKA
                     QTVYNRSISNENIITYIVPCIMNESVIIYQITTSFFNRLFAENEDNIESSVLLLGMDN
                     RLIYAYNTDKDFLNNLETIIAVDTGVMKEVAVAGNDYYIYKTVSDLKSFVLMEIIPKS
                     FIEEHVNKFSQIYHVGLIVIGLLGMLVIYWQTIANYNPIKKLGNTARAANLKIPEHLN
                     ALESIEFAIENLSKNSSKIPERDVEAMRELKLFHLLRGQYKSVDEFNNDCKGLDMFVL
                     GNRFRVVVLLTEFNSEYALPEPQIFDQLRDMMRIDYEVFMLEYLEQFSYIFILSREEH
                     SSIEHSDGEYSKNELEKRLRFLQMHYKEITGSSLTIGIGYEYNNTGDILLSYNEAKNA
                     VNYKLVRGADSIIFHNDIHRNISPIHYAYPKAELDSLYYAIINGNTTKIEFITEILIS
                     LLRKEYHSLFLAKCLCYDVINTALRAVHDINLSISFFDKYNKSEFTSIDEFIQVVEMI
                     CSEIIECMDGNKKKYASLDINAVTAYINENYSDPNFCVSSLADHFNMSISNFSHQFKS
                     YMSQNISAYINNIRIKTAKTLLASTELSVSDIALQVGYIHSSSFVRKFKQAVGI"
BASE COUNT         6719 a         3874 c         3778 g         6725 t
ORIGIN      
        1 aaattgacct ggcccggcac gatttccacc gtaacttcct catcgctttc atccagtaat
       61 ttgatcagtt cgatcaccgt cttgcgcggc aggatcaatt cctgtttttc atagttgcgg
      121 ttcaattcgg ccgaggtgaa actcagacga tggccgtcgg taccaaccag gttgagacgg
      181 ttggcattga cttcgaacag cagaccattc agatagtaac ggatatcctg ttgcgccatg
      241 gcgaattcaa cctgtttcag caggcgcttc aatacacctt ggctgattgt gactacgctt
      301 ggctctgcac cggcagcctt ggtcatgacc gggtaatcgc tggctggcaa ggtctgcaga
      361 ttaaagcggc tcttgcctgc cttgacctgc atgcggctgt cggttgttgc catgttgatt
      421 tcggcgtttt ccggcaaggc acggcagata tccagtagct tctttgccga aacagtcgtc
      481 gataactcac ctcctgcatc gctgtcgatc gacaaggaaa tctgcatttc caggtcggtc
      541 gcggtcaata cgatgctgtt ttgtctggct tccagtaaaa gattggaaag tatcggcagt
      601 gtatggcgtc tttcaactat accgctgacg gcagaaagcg gcttcaacag ggtatctcta
      661 tttattttga tgttcattgt tcaaaaaccc gtctgtgtta atgtcgattc aattaataga
      721 ataagtaata cataaaagct aataacatat agtaatgcac agcacatata agtatataat
      781 cgaaagatat gatcaaaaaa tacaaaaaac ctacaagtaa atacttgtag gttttttatg
      841 gcgtgcctgg agggattcga acccccggcc tacggattag aagtccgttg ctctatccag
      901 ctgagccaca ggcacaaaat ggagcgggtg aagggaatcg gaccctcgca atcagcttgg
      961 aaggctgatg ttctaccatt gaactacacc cgcatatcta ctaacctgtt gactttttct
     1021 attatacagg ttaagtttta ttttgtcaac atgtatggga atattttttt tttggacttt
     1081 catagattta ttagggaaat ttcctgggaa cagtccttta cttatacaag aaaaaaaggt
     1141 gctatctaca ttagcacctt tagataaatg atttgatata attattccca ttaagtttga
     1201 atctacttat tcaacaacaa tttcaaattg catttcataa tctgaaaatt cgggtacttc
     1261 aaattgataa tcattgaaat tcttattcat caatacatcg atagcattct caacatcttt
     1321 gtttcttaat aaagttacaa cttcagtgga gaaatctcca atctccttaa tagttgtagc
     1381 attatttacg aatactgatg tatcgaaatt cttatagaaa tcaattgtat cagcctgtat
     1441 ttctgatagg ttgtcaacat attctctgaa aatactgtaa ttatcaggat ctattgtagc
     1501 tatgaagtct tggccgaatt ttccgccaaa ttcaacaaag tgaacaagat ccttgactaa
     1561 ttgtgtatct acactggaga gcgcatcaac aattagttct cttacatcac cagcctggtt
     1621 ggcgtagtcc atgattgtat tagtgatttc attaatagat tctacgtcaa ttttaccggc
     1681 agttaaagct acattattca ttgtatcatc aagaagtccc atatattcat tatataatcc
     1741 tacatactca tcaatagtat cagctatatc accgaatgta cctgccggta tataatctga
     1801 tacaagatta ataagtcttg agttcctgaa tattttattc tgcaatgatg caactgtttc
     1861 ctcaatagaa tcagttgaaa aactgaggga tgcaaaagtg ctttccaatt catccttcat
     1921 atcaagaatc tcttcataca tgccataata ttcctctacg gtacctacat attcattaat
     1981 tttctctgaa ggaataaacc aggaaaccag atcctttagt ttctggtcat aaagaatttt
     2041 atccttggtt tcctctatag aaccataata ttttactgct tcaagaggag cagcccagtc
     2101 ggcaatggct ttctgagcta tattgaattg ttcggaataa tcagataagt catcgacaag
     2161 tgcaaattgt attgcactat taagaagctc accatgattc agaacaaaat caacaaattc
     2221 attgactttt acatctgaaa gcagagctgt tgagtctaaa attcttgtta taaagttact
     2281 tatgaattcc gattgaccag ccttaaaagt gtcaatttga tccaaagcct gatttctgta
     2341 atccattgcc atttccttaa ttttatcggg taaaagttca atataggctt taacaatacc
     2401 tgaaccaaat tgtacatctt ttaccttgaa aatgtccgga aggtcaaaat ctgcatattt
     2461 taatgaatat gagagggcat ctgcaggaat ctgattggtt ataagtccgg gggctttttt
     2521 gtttccaaga ttaagttgag aagctgttat atcaataccg tcttcagtaa gattgatatc
     2581 aagctttgcg ctaagtggaa cattcaaaga gccatatttc ccgttaatgt ataatctttg
     2641 atcttttgtg ttgaatagaa tttcgttgat tgaaaactta tcaggaagtg caatatcaag
     2701 gttttcaaaa ttatctttta ttagcgagtt gatcaaatcc tggttaagct caatttctac
     2761 agtttttgag tcggattcat aatatgtatg ttttgttgcg atttccttga ctaggtcttc
     2821 aggctgtaca taaacaaaac tgcaatcata tgtagaatta tgaatgctta gtacagtacc
     2881 aatacctgct gcaagaataa ccaggattac tgctgctatt attaaaccaa ttagcagccc
     2941 ttttttaata gccattaaaa cacccccaaa tcaataagac gacaagtaaa ctatatttat
     3001 acataaacca catctgctgc taagatgtgt tatccattat ttaacctcct ttcgctaaaa
     3061 ataatttatg tagaattaaa taagtgaact taaagcccac ttaattccta ctccattgtt
     3121 tagcactatc tgtcttttct attagtttca aagttatcta caaaaaaatt gaagtcataa
     3181 tcctgatgta tgtactatac attctatatt attttataaa ggtatttata agtttattat
     3241 gtaaatgtga tatattcaca ttaatatatt tatattaaat tttaattaaa attattttac
     3301 aatatcatct aaggccattt taacagtatt tgagcatagt gtcaatagaa taaatataaa
     3361 gaatgacaag tttattatat gtatatgcgc taatgtaaac aaacaatccg taagctccgt
     3421 tcaaattttt tggggacact ttacaaagta aaggaaatac ctgttacttg agagaaaagt
     3481 gccccctgcc ttatatacac ttgggaaatc ttatactgcc atattccaca ttctattttt
     3541 tgtcttgctc ccttatttga actctacgaa ttttaccgct tattgttttg ggaagttcat
     3601 ctacaaactc aataattctt ggatatttgt atggagcagt aacctttttt acgtgatctt
     3661 gaagttcggc tacaaggtca ttacctggtt tgtagttttt agtaagaact actgtagctt
     3721 ttaccacctg ccccctaata ggatcaggta ctgcggtgat ggcgcattcc aaaactgcag
     3781 ggtgctcaag gagagcactc tcaacttcga aaggaccaat tctgtatcct gaacttttaa
     3841 ttacatcatc tgcacggcct acaaaccaga agtaaccgtc ttcatctttc catgcaatat
     3901 ctcccgtata ataaatgtta tcgtgccata cttttaatgt caatttcggg tcgcgataat
     3961 aaccgccgaa tattcctaca ggtattctct tgtcagtacg aactactatc tgaccttcct
     4021 caccaacatc acaggaattt ccttcttcgt caattaaatc aatatcatag cctggagcag
     4081 gctttcccat tgaaccgggc tttggttcca tccaagggaa agtgcctatt gcaagagtta
     4141 actcagtctg accgtagcct tccataagtt taaggccggt agcttttaaa aattgattat
     4201 aaacttccgg atttaaaggt tcaccggcta tggaacagtg tttcaactta cttaaatcaa
     4261 actttgtcag atcttcttta atcaaaaacc tgtaaattgt aggaggagca caaaaagttg
     4321 tgacaccgta cttaactatt acatcaagca aatcctttgg aacaaatttt tcataatcat
     4381 aaacaaaaac agcactgccg caaatccatt gtccatatat tttgccccat acggcttttg
     4441 cccatcccgt gtcggcaaca gtgagatgaa ggccgccatc tgaaacgtcg tgccagtatt
     4501 tggcggttac aatatggcca agaggataaa taaagttatg ctgtaccatt ttgggcattc
     4561 ctgtagttcc tgatgtaaag tataaaagag aaatgtcatt attagtagta gcttcttctc
     4621 caacaggcct ggtaaaatca cttgatgatt tgttaatttc ttcatgcagg ttgaaccaac
     4681 cttttctatt cccatctgcc aggactttgt gtttaacagt aggagaatct tcaagagaat
     4741 tttctatatg acctattact tcgtcttctg ctacagtaat aatcatctta atgcttgcag
     4801 cattgtttct gtatacaata tccttgggtg taagcaaatg ggtagccggt atggctattg
     4861 caccaatctt gtgaagagcc agaagacaaa accagaattc ataccgcctt ttcaatatga
     4921 gcattaccga gtcgcctttt tttattccta atgacttaaa aaaattcgct gctttgttgc
     4981 tgtattcttt caattgacca aaagtgaaaa atgcctcctc accactatca ttgcaccaca
     5041 ctatagccag tttgtccgga tttccagagg ctatttcatc aactatgtca tatgcaaaat
     5101 tgaagttttc cggaacattt attttaaagt tttctgtaaa ttcttcatga ttggtgaaat
     5161 cattttgtga aataaagttt agctttttga gcattgaata ttacccccta attagtcttg
     5221 agttcactgt gtgattattg ccagaaattt tgcaggtctc ccgcctaaag ctttcattcc
     5281 atgttttata ccggaatcaa aaaataaaga atctccttct ttaagtataa gcgtatggcc
     5341 atcaatagta atttgcattt ccccttccag cacatagtta aattcctgac ctggatgaga
     5401 gttgtaaccg gcatctccat tattatccga attacctgaa ttatccgaat tatccgaatc
     5461 aggttctaca gttaccagaa aaggttcaat ttttttattt aaaaaattat atgcaaggct
     5521 gtgatactta tatgcttttc ttctctcaac actgacgcct ttaccttttc tcacaagaga
     5581 gtatgtctga agtttgggat tatcacctgt aagaatagca gttagttcta cattgaaccg
     5641 accagctatt ttatatagaa aactgacggg gatatctact tctccgcttt cataggcttc
     5701 ataccgtcct tttggtatac ctaattcttc tgccagggtt tctagtgaaa tgctatatat
     5761 ttctctcagt tcttttattc tgacagcaat ctgttttatt tgattagaca taataacacc
     5821 ctttctaaaa ctatataata ttaactgagc tacactattt taaaatgtac aggaaagtat
     5881 gtatttcctt tttatataat ataccactat gcaattcaga tgtaaattat ctgcatagaa
     5941 atttccataa gtaattctta tagttaaaag aatattttac taacttattt tactatagta
     6001 taattatttc attaaaaagg ataaatacaa gtacattttg aaatgactaa atactaaaga
     6061 agtcgtagag caataggtta tatatatgaa agaaggtgct gatatgaaag aatgtgctag
     6121 tatgaaagaa tgtgctgata tgaaagaagg tgctagtata aaagatatgg taagacgtat
     6181 ttttgtagaa accaaaaatg agtatgatgt agaatcacag caactctatg aggaaatcac
     6241 cgggtttctt ggaattgaaa atttaaaatc tgtaaaagtt ttttccaagt atgatattca
     6301 gggaattaca gaccagcaat atttggaagt cagaaacaga gttttatcag agcctccaat
     6361 taataatgta tatgaagaaa atacaggctt cgaagaacat gttatgcttg aaaaagaaat
     6421 tagaatcatt gcaatggaat atctccctgg acagtatgac caaaaggctg atattacgtc
     6481 acaatgtatt cagataatta taggtgggcc aaggccaatt gtgaaaacag ccagaataat
     6541 cgttcttaaa ggaaaaatat cagatgttga attcagcaga attaaaaact attatattaa
     6601 tcctgttgaa agcaaagaga catctttaga aaagctatca aagcttgaca gtgatattaa
     6661 agaacctgac gatataaaaa ttcttgaagg ttttattaat gtatcggatt ctgaaatcga
     6721 agaatacgga aataaaatag ggtttgcaat gcctggtgaa gatcttctgt tctgtagaga
     6781 ttattttaaa aatcaggaaa aacgcgaccc tactatgaca gaaattaaag ttctggatac
     6841 ttactggtct gaccactgca ggcatactac ctttatgaca agaatacaag atattgagtt
     6901 tgataaagga ccatactatg aagttattaa cagagcattc caggaatatc tcaaatccag
     6961 agaatatgtt tattcggaca agcccaaaga tatatgcctt atggaccttg caaccataaa
     7021 tatgaaagag caaaggaaaa ggggactgct ggaagatctt gaagtgtcag aagaagtaaa
     7081 tgccagcagt ataactgtta gtgttgatgt agagggaatg actgaagaat ggcttgtaat
     7141 gtttaaaaat gaaacccata accatcctac ggaaattgaa cctttcggag gagctgcaac
     7201 ctgtcttgga ggaggaataa gagacccgct ctcaggaagg gcatatgtat accaggctat
     7261 gagaattacg ggcagcggcg acccaaggac aaaaattgaa gataccctgc caggaaagct
     7321 tccccaaagg aaaattacta caactgcagc agcgggatat agttcatatg gtaatcagat
     7381 aggcatttca tcaggacatc tttcagaaat ttatgatgaa ggctttgtgg caaagagaat
     7441 ggaacttgga gctgtaatag gggcagcacc aaagagaaat gttgttagag aaaatccatg
     7501 tacgggtgat gtcattattc ttttaggcgg aagaacaggc cgtgatggct gtggtggtgc
     7561 tacaggatct tcgaaggaac atactgagga atctctgctt acaagtgggg cagaagtcca
     7621 aaaagggaat ccgctcatag aaagaaatat ccttcgcatg tttagaaagc ctgaagtaag
     7681 ccgtatgatc aaaagatgca acgattttgg tgcgggcggg gtttcagttg ccataggtga
     7741 acttgcagat ggactggaca taaatcttga taaagtaccg gtaaaatatg cagggcttaa
     7801 tgggactgaa ctggcaatat cagaatccca ggagcgtatg gctgttatgg tgtctaagga
     7861 aaatgaagct cgatttattg aaatggcagc tgaagaaaat ctcgaagcta ctacagttgc
     7921 agttgttacg gaggaaccca ggcttaaaat aagatggagg ggtaaaacca ttgttgattt
     7981 aagcagggag tttctaaatt ccagtggtat aaggaaaaac accaatgtta aagtattgag
     8041 ccctgttaat gaaaataatt actttttctc tctgccttat gaaatagata agagcctcga
     8101 agctggtgat atagaaaagg cctggatgag caatctgaaa agacttaata tatgcagcca
     8161 aaaagggctg atggagagat ttgatagtac tgtaggagga gatactgttt taatgcctat
     8221 gggaggcaaa tatcagttaa gccctgcaga aggtatggta gcaaggatac cggtgcttga
     8281 gggagaaaca tccacagcca ctgttatgac ctatggatat aacccttcaa taggaaattg
     8341 gagccctttt catggtgctt tatattccat agtggaggct gttgcaaaag ttgctgccat
     8401 tggaggggat tacaagaaga taagattaag ccttcaggaa tattttgaaa gactgggcag
     8461 tgatcccgaa aagtggggga aaccttttgt tgctttactc ggagcatatt atgctcagaa
     8521 aaaccttgga ctgccttcta tcggtggaaa ggacagtatg tcgggtacct tcgagaatat
     8581 acatgttcct ccaacagttg ttgcgtttgc ggtaaatact ttaaatataa atagtgttat
     8641 ttcaactgag ttcaaaaagg ctgggagcaa ggttatttgt gtaccattaa gacaagataa
     8701 aaataatatt ccggatttcg agggtatggg taaaaactat gcccaaatac atgagcttat
     8761 aaagacagga aaagttttag cttcgtcaac agtaaaagaa ggtggactgg ctgctgctgt
     8821 aagcaagatg tgtttcggca ataagattgg gtttgctctt tcacatgtaa aatccccgca
     8881 aaatctattt gcgcctgatt atggttctat tgttctggaa cttgacaata caggtgattt
     8941 gacccagatg gaacagaacc tccagggact tgattattat gagcttggaa ctacaataga
     9001 tgagcagaat ataattatta ataatgttgt gataagcctg gacaaggcaa tatccagttg
     9061 ggaattagtg cttaatgata ttttccctgt aaagactgaa aaagttgaaa cttgcgcaca
     9121 cagtatttat gatagtatat cagaaaataa aagcacttca gaactaaaag ccggtaattt
     9181 tacaggaaga gcaaagctcg ggaaaactgc caaaccttta atctttattc ctgtattcca
     9241 tggaacatct ggtgaatatg aggcagcaca agcatttgaa aaagcaggtg ggacagttga
     9301 tacatttgta ttccgcagtt taacaggaga gcacattaag caatctatag atattataga
     9361 agagaaaatc aataacagtc agatactaat gcttccaggg ggattaagtg gagatttgca
     9421 gctaggaggc tccggacgat atatggcagc tgtcctgggc aacccgtata ttaaagatgc
     9481 tatcaaccgt ttgctgaatg agcgtcatgg tcttatactt ggactaggta taggttttca
     9541 ggcattgtta aaacttggtc tgcttccata tggagaattc agagaaatga atgaatccag
     9601 tcctacattg gcggttaata ttaatgggaa ttttgtctct cgtatggttt ggacaaaagt
     9661 tacatcaact atgtcaccat ggtttgcaaa cactaaggca ggagatatac accttgtacc
     9721 tatagcctgt ggagaaggaa gatttgttgc cggaacttca gatattgaat tattagctaa
     9781 aaatgggcaa atcgcatcaa catatgttac caactgcgga agtccggctt gtgatacagg
     9841 cagtaatcca acaggctcca tgaatgcgat tgatggaata accagccctg atggaagaat
     9901 cctgggtatg gttacacatc ctgaaagaat agacacaaat acagcactga atgtaccggg
     9961 gaataaagaa cagaagatat ttgaggcagg tgtgaaatat tttggttggt gagttgtaga
    10021 actattaagt tattcttgaa gtattttctc aaagacgagg gattcctaac ctttccatca
    10081 cgcaggcttt tgcagcttgc aatatgaact ataacggcca ttccgcagat tatttagaga
    10141 gaaggttggc gtttccccat ctgtatatcg gaaaatgtca gagtcaaatg aattgtccta
    10201 ctaagcaata agagggagcc aggctttggt taaacacaaa agcccggctt ctttttaatt
    10261 tcgaaataac atttttcgta ctaattataa gcatattcaa cctatcctta cagccagcct
    10321 gttgataaaa tattaatagc agttatgcaa aatataagca ggctggatgg aaaagaccat
    10381 gacttaggag gtctttgcta agggccaaac ggtaaagtta taccactgac gaaagtatac
    10441 ttattgaatg ggatcctgtc attgatgaag atattaatag cagcaatgct tataacgaag
    10501 tttgttttta tattgagaag ggatcgtcaa gcttacttaa ccattctttt aattcaagct
    10561 ccatttggaa agctaaatca ttttctgcag cagtttcaaa acatagcgga tttaattgat
    10621 aaggatccgt ttcattgtcg taaagtagtc ttttagtttt ttctccggga aagtatcctt
    10681 tactaactac atatgtatac ctataggtac gtacacccct ccaacctaat gtttttggat
    10741 ctatcccttt agcacttagt tcttttagaa aaacatccct accgggacaa gcacaaataa
    10801 aagcagatgt tacaggctgt gttttttcct tgcggattac cggtgaaagg tcagtgcctt
    10861 caaccgaagc cggaaccgga atattcatta attgcagtaa agtaggcatt acatctgcgc
    10921 tgcctaaaag agttttctcc ctgcctggca gtatccggga aggccacctg ataacaaaag
    10981 gaattcctat agactcttca agccatacat gttttgccat taagccatgt gaacccatca
    11041 tatctccatg atctgaagac agaacaacta tagtatcatc ttctatacct tgttctttaa
    11101 ggactttcag taatctcccg aaattttcat caagaccgga aactgctgca taataatcct
    11161 tggtgaacct ggtcagagtc tcaaaacctt ctccaatctt ttcatatgta tgcgtataca
    11221 agccttctgc ttttacatta ggtctgaagc taatatcttt atccttgtat aattctttat
    11281 atttttccgg taacatatga taaggactat ggggagggtt ccaggaaata aaaagggcaa
    11341 aaggattttt ttcatatttt tttgtatgat catttatgta ctttatagct acatctgttt
    11401 catgctcagg tgaccattga ttgatatgta ttgctttatt gctgtcctgc caataatgag
    11461 gtgccatatg ctcatcattt gcaccgtatg aataccaaaa gtcgaaacca tgtctcttag
    11521 gaccgggagg agtataggca tcccatcctt ttgcaccgga ttcgggattt tcagaaaaat
    11581 tgcgttcaga tgagtcaaga tgccacttgc caatgtagcc tgtagcataa ccttgttttt
    11641 tcaacacatc actaatacat atttcatggg ttccaagggc aacatccacg ccgggtttac
    11701 aatttgtaaa cataccggta gaaagagggt aacgtcccgt cataagagat gccctgtggg
    11761 gagagcatag ggggaatgta ctgatagcat tttcaaatat gagactttca tctgcaaatt
    11821 tatccatatt tggagtaaca acaggatctt cattcataaa acctactgcc tgacgtctcc
    11881 attgatcggc aaaaacaaat ataagatttg gtcttttttc gttcatttaa agctccattc
    11941 ctgctgcgca cgaaatgaat tcgccctttc aggaacacaa ctttttaaga aaaagttttc
    12001 atgggcttaa attcatttca gtcgcgagtg caatttattt ttttaaaatt cttgtgcaaa
    12061 aatatgcaca tgcgcattag ttttcagaaa tttaccaatt tgttttaaat taagtcacct
    12121 tcaaattaaa taaataaatc atattaaaat acaaaatttg accaagaggc atgtcttagc
    12181 aaatcttcgt caatctcaag gccaaatccg ggtaatagtt ttgggactgt tattgcactt
    12241 ccgtccatcc gaacagtagc ttgtgctttt actgagttaa gaaagccatc tgattcacct
    12301 ggttttacca atgcttccac ccaatccgca cccaatccaa ttgcaatctg ttgccatgct
    12361 gtattagcag gacccagcaa actatcatct cctatcatta atttaccgcc aatttcctta
    12421 atcttttttc ccaatttaac tgccccatga atggaaccgg tacatcccgg atgaatatta
    12481 tagattcttc ccattccggg aagaatagtc tttatagctg taacggctag cctcaaggga
    12541 taatcaggta tcaaatcaag accgcctgtc atattctgca attcaaccca ttgagcattg
    12601 gtaagcatgg caggatcttc acaagcatta agaccattag catgaagttt tattaattga
    12661 tttgctatat catgcattcc ttcatcagct aatttttctc tgtaaccaca attcacatca
    12721 cctattatgt agggttcttc tcccagtaca tcccttatag cccttacaag ctctatatca
    12781 agctcaattt taccaaaaag ttttagcttt acatgcgtag ttaatttctc atttattgct
    12841 ttttttgcca gttccttaac ctgtgtggga tcatcatgca aaattacata aactccaggt
    12901 acagagtttt taacttcatc ccaattaagc tctaaaagtt tgccagctgg ttccccgata
    12961 atatgtccag caagatcaag taaagccata tcagccatct cacatctgcg gcttccccaa
    13021 tctgtaagtt tcctttctat atactgaata gcctccaaga tggaaagccc atttaattca
    13081 tggaaatatt gcccccattc agataggctt atgtttttat tatttacgga cattacattt
    13141 tcaccccaac cgcagtaatc tccgctggta atttctataa atgcgtgttg acggcttttc
    13201 catgttccaa aactaaaatg tctttcaaca tcggaatcat atctgaataa ctcaatctta
    13261 tcgattttat ttttagctaa agctgatcca gtcattatga gatctccccc aatccataca
    13321 taaatagtgc atcttactta taatttcagc ataagatgct ttgcctgcca aattatccat
    13381 ttccagagga tctttttcaa gatcatacaa ttcggcagaa tcacgtaaat catatacata
    13441 tttatatctc ctgctaataa ccgcacgccg aatatcccca acggttgggt ttcctgaata
    13501 ctggcagaat aagttccttt cttcaggtgc aactccccgg gtaatcgggt ctgccagtga
    13561 aataccatct aaatcacacg gaatagacaa gccggtcata tccaatatag taggtaatat
    13621 atcaagatga gaaaccggag tatcgataat atgtctatta atgcctgttc tttcggtcag
    13681 aaaatttggg cttttaacaa taaagggtac attaatagct tgttcataca tctccatttt
    13741 ttggtacata ttgtgctgac ccagatgatc cccgtgatct gaggtgaaaa caatgagcgt
    13801 actatccatt ttgccgtttt tttcaagctc ctgaaatatt ctacctattg cagcatcaac
    13861 aagatttaaa agccctaaat gtgctgccca tactttcctc cattgttcca aggtcaaacc
    13921 ttcagctagt tgtgctgcaa tacctcttct tctgtttgct ggttctcctt ttgcagctat
    13981 tccgatatta ccgggtagtt gaagattatc cgggttaaac atggacgcat atggttcagg
    14041 tactgttaaa ggtggatgag gtgcccagaa gtacacaaaa agagcaaatg gattttcatg
    14101 ttctttttga ataaattcaa tggctttccc tgaaaaataa ttatctttaa aatatttttc
    14161 aggatggggc caaatagaag tcatagcatt ggaatatttc ttttctacat acttatcatc
    14221 ttgaaattcc atcacttgtt tttcaaaata ttctgttctt cttatattaa tatcaagctc
    14281 ttttacatat tctgcataat cattatcatc aatccacata tcaaagtgca tacggtcctt
    14341 aagagaaggc tttacttgta tatggtttac acctacatgt cctacagtat aaccagcctc
    14401 ttccaaatac tgatgaatgg ttttgtgatt accggctctt aaccctttaa catcattaaa
    14461 cacaacacca ttttttgtag gatattttcc agttgccagt gctgttcttg caggcacaca
    14521 caagggacat gtgttatatg cacgtgtaaa cactgctgat tgttctgcca gcctgttaag
    14581 gttaggtgaa acctccccgt tatcctgaac catgcctata ctgtctcttc tctgatgatc
    14641 ggtcattata aataaaatat caggtttatc catcttgtta ttcatagtct gttttctaca
    14701 tacaaggtgc atttttaaat atgaagagat gtttatcagg gactaatatt acagtaccta
    14761 gagtctccac ttatgccaat tgccaatatt ttcttcatat aaataaaata tacccctgtg
    14821 tatctccttt taaagattta acatagaaat tcagtggggg attataaagc tcccactgaa
    14881 tttcctaaca acgagaattc gatttatttc ttttggttgt cataatattc ttgccaaagc
    14941 tgcacatatc tatcaacatt tagagatttg caagtcttca gatgttcatt ccactgggca
    15001 tcagtgactc cttttaaaat gctgtttgaa acaaaattcg ctaaatatgc gtcaatctca
    15061 actagtaata aatttttctc attagctgtc tcaatatttt caaagttaga ctgaagagtt
    15121 tcagctggaa aatacttttg gtacaaatcg gtaattgcat caagacgtat ttttgtacgt
    15181 ggtgctaaaa gacgtttata aagaccttct tcggcagtca tgaaccagtt agcctgagaa
    15241 gcaggtgcta cagtatggcg gtattcaccc cagcttgcgc cttgtggcag tttatcatta
    15301 ttctgaatcc ataacccgtt ttcattgaac ttccatgctt ctccttcagg accaaaagac
    15361 cagcgcattt tattttccca ggaactgtta atataatcat gccacctaat cattgcaata
    15421 ggatttttgc aagacttggt tacaacaaat cctgtaatat ttccgccctt ttgaccttcc
    15481 ttattccaga ggactttgcc tgcacctcca tctattggtt caataggtat ataatggttc
    15541 atattatcac cggtagctgt atcagcatta tagcttagaa atgatccaag aagcagctcc
    15601 ttgttttttc ctttggcttg caactgctga agagcctgac taaatccttc aacatccatc
    15661 aaaccagctt gaaccaatga gttaaaccat actagagcat cacggaactc attttttatt
    15721 gatggaaaaa tgacttttcc attttctaca attagatgct gggtattatc aagaactcca
    15781 aaggttccaa agaatgaatt taatgaatac tgagcatctt gttggcagaa agaaaaagga
    15841 atttcatcat ttttcccatt tttattcata tcattgtctt taaatgcttt aagaaccata
    15901 taaaactcac ttgttgtacg cggcacttgc atacccaaag catccagcca tgttttgttg
    15961 atataaagca aggaatttgt tacaccccaa gcagattctt cactcgtgtt aaaagaatag
    16021 attttgccat ccggtgtagt taatgcagtt ttgatatccg ggcgttcgtt ccagagtttc
    16081 ttaatgctgg gggcatattt gtatacatag tctcctatgg gtacaagtga atcaatgttt
    16141 tgcattagca tcgatgtgct gatgccactt acaattgcat ccggaagact tttggaagca
    16201 aaagaaagat tcactttttc attccagcct gagggagcaa tttcttgcca tgtaatagat
    16261 atattggttt ccttttctac ttcttgaaca ggtaattttt cagggtagga attcttgctg
    16321 agagcatttt tcctgatcat aactgtaaaa ttttcttttt ctttcaggat gggtataccc
    16381 gatttatata ggttcccctc aagattagtg tcagtatcat tgttttgagg aatagtagct
    16441 tttgtttctt gaaattcagt ctttacgcag cctgtaaaag cttgaattac cagaatagct
    16501 gccaacaaaa ataaacatgt tttcttgaac ttcattttaa tctccccttc ttttattatt
    16561 tttaatgttt ttattatttt agcttgctta aagcaattat aatacttttt catatttcac
    16621 cttgcttaaa gcaattataa tgtttttttt agcccttaac tgatccaatc ataactccct
    16681 tgataaaata tctttgtaaa aagggataca ttatcaaaac tggaagtgta gaaacaatga
    16741 taactgcata tttgagagat tcagctattc tctgtgcatc acctatggat tcaatatctg
    16801 tagtgtcgct cacaagcatc tggcttgcaa taagaatatc tctcaagatt aattgcagag
    16861 gatatagttt ttcgttattt aaatagataa gagcactaaa aaatgaattc cagtgaccta
    16921 ctgcataata gagggtcagt actgatataa ttgatttgga tagcggcaca actattttta
    16981 agaaaaatat gccattccca catccatcaa taaatgccgc ttcctgaagc tctactgaca
    17041 tagtattttg gaaaaatgat tttgcgatta taatattata tactgaaatg gagcctaaaa
    17101 taatcattac aagaggggta tttataaggt gtaatttatc tacaaccatg tatgtgggaa
    17161 taagtcctcc cccaaaatac atagtaaaga cgaatatacc cgttatggga cctttgccaa
    17221 caagatcctt gcgggaaagt gagtatccgg cgaggattgt tgctgttaca cctaaaagag
    17281 taccaaaaaa agtatatatt attgtattat aatatccccg ccaaatccgg ttatccataa
    17341 aaactcttat ataaccctcg aagtttaaaa gcctgtcctt gccaccaatt ggatataaga
    17401 gcattgaacc tgtgttaaca gcatccggat cacttaaaga tgctataaca atgaaataga
    17461 ggggataaac ggtaataatc aatataagaa ttaataataa atttacagca aaatcaaata
    17521 tcccatctga tgagtgtttt aatagattca attcggctgt ttttcttttc atgtttgcac
    17581 cccttaccat aaactagtat ttgaaatact acgggcaaat ctattaacca gaaccagtag
    17641 agtgaaattg ataacattgt tgaaaaggcc aattgctgtt gaatagctgt attgagcgtt
    17701 aataagacct actttgtatg tgtatgtaga tataacctcg gaagcattca ggtttagatt
    17761 gttttgcatg agcaatgctt tttcaaagcc tacattcata atattaccca tacgcataat
    17821 caacagtatt atagctgtag gaagcaatgc aggcacatca atatgccaaa ttctctttat
    17881 ttttgatgct ccgtcaatta ctgctgcttc atgtagctca ggacttacat ttgttaaaac
    17941 agctatataa attatagaat tccatcctgc attttgccaa acacctgacc agacaaaaat
    18001 gtgcttgaaa tatacctctt cccccatgaa aaaaaccggc ttcaaattca tgtaggaaag
    18061 gagtttgtta acaacaccat tcccgggaga aagaaaaagt ataagcatac caaccataac
    18121 cactgtagat ataaaatgtg gagcataagt tgcagtttga gcaaatttgc ttagcttttt
    18181 catggtactg tagtttaata taagagctaa taaaatagga actggaaaac ctgcaaccaa
    18241 ctgatatagg tttattgtga gagtattttt aataagattc caaaatctat aggaattaaa
    18301 aaaagtagta aagtgcttaa aacctatcca tgggctgttc catatccctt tagttgaaga
    18361 aaaattctta aatgcaatct gaattccgta cattggccag taagcaaata taattatgta
    18421 gattaaagca ggtaaaagaa aaatataaag catataatta cgagatacca ttttccattt
    18481 ggatctatag aactcatgtt ttccatacgt tttcattttc gatggttcat attgcctgga
    18541 taaaacattc atagtatcaa cctccagaaa tcttgaagaa cttgcctata taaagaatat
    18601 cgatttttgc tgaaaaagta aatgtacatt attgccaatg caatatactt tttttgaaaa
    18661 agctaaatag gcatctttaa agcattatag agagaaggct ttctgattgc ttaaatttga
    18721 attaagcatt aaccatattt gaaatgcacc tcattttgcg ctgtgaacat tgaaattatt
    18781 tgtttttcgt gttaatatat taatatgtaa ccggtatatg gtctatatac agcaaaaaca
    18841 cagtaatatg tataaaaata gataatttgc agaaaatgag gaatttatat gaagaagaac
    18901 agcatactgt ttaaatattt aatttcttat ctattggttt tgtttattcc cttgtttata
    18961 cttagtgcca taattaagta ttattttatt aaaacttaca gcgaatctgt cttgaaacag
    19021 aaagccatgc aaatacaaca aataaaatca tctattgata tacagctaaa tcagcttcac
    19081 aattttgcta ttcagtcttc aagtaaaaat atattttcaa gatataatat agaaaacaac
    19141 ccgtcatctt ttaatgatat tatgaaagaa atacgttatt ttatacatat taacgctaat
    19201 ctgcaggaag taatatttca tttaaaagac agtgatatct attatatgag ctcgggaaca
    19261 agtaaaaatg attatatatt tacaaacatt atgaattatg aggaaattag caaagataat
    19321 agtttggctt tcttttcaaa cctggacgca cctatttggc ttaaagcaca gactgtatat
    19381 aacagaagta tcagcaatga aaatattata acatatattg taccttgtat tatgaatgaa
    19441 agtgtaataa tataccaaat aactacttct tttttcaata ggctttttgc agaaaatgaa
    19501 gataacatag aatcttcggt acttttatta ggaatggaca ataggctgat ttacgcatat
    19561 aatactgaca aagatttttt gaataattta gaaacaatta tcgcagttga taccggagtt
    19621 atgaaagaag ttgctgtcgc tggtaatgat tattatatat ataaaacagt ttctgatttg
    19681 aaatcctttg tgttaatgga aataatacct aaatctttta ttgaagagca tgttaacaaa
    19741 ttttcccaaa tatatcatgt gggtttaata gttattgggc tgctggggat gcttgttatt
    19801 tattggcaaa ccatagcaaa ctataatcca ataaaaaagc ttggtaatac agctcgtgcg
    19861 gctaatttga aaatcccgga gcatttaaat gcactcgaat caatagaatt tgcaatagaa
    19921 aatctttcta aaaattccag taaaattccc gaaagagatg tggaagctat gcgtgaatta
    19981 aagctttttc atcttttgag aggacagtac aaaagtgttg atgagtttaa caatgattgc
    20041 aaaggtttag acatgtttgt cctaggcaac cgattccgag ttgtggtcct tcttaccgag
    20101 tttaatagtg aatatgcact acctgagcct caaatatttg atcaacttcg ggatatgatg
    20161 cgaattgact atgaggtatt catgcttgaa tatcttgagc aattcagtta tatatttata
    20221 ttatcaaggg aggaacatag cagcatagaa catagtgatg gagaatacag caaaaatgaa
    20281 ctcgaaaaga ggcttcgatt tttgcagatg cactacaaag aaattacggg gagcagcctt
    20341 actataggta tcggttatga atataataat accggtgata ttcttctttc ctacaatgag
    20401 gctaaaaatg ctgtaaatta taaacttgta cgaggtgccg acagcattat ttttcacaat
    20461 gatatacata gaaacatcag tcccattcat tatgcctatc ctaaagctga acttgattct
    20521 ttgtactatg caataatcaa tggaaatact actaaaatcg agtttataac tgaaatactc
    20581 ataagccttc ttcgcaaaga atatcatagc ctttttctcg ccaaatgttt atgctatgat
    20641 gttataaata cagcacttag ggcagttcat gacataaatc tttctatctc attttttgat
    20701 aagtataata aaagcgaatt tacttccatt gatgaattca tacaagttgt agagatgata
    20761 tgcagtgaaa ttattgagtg catggatgga aacaaaaaga aatatgcttc acttgatata
    20821 aatgcggtta ccgcttatat taatgaaaat tactctgatc ctaatttctg tgtcagcagt
    20881 ttagcagatc actttaacat gtcaatatca aactttagcc accagttcaa aagctatatg
    20941 agtcagaata tatctgcata cataaataac atacgcataa aaacggcaaa aaccttgctt
    21001 gcatcaactg agctgtctgt aagtgatatt gctttacaag ttggctatat ccattcatca
    21061 agctttgttc ggaaattcaa acaagctgta ggcata
//
LOCUS       DAOSDD010000056        20599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1109_length_20599_cov_4.771028, whole genome shotgun sequence.
ACCESSION   DAOSDD010000056 DAOSDD010000000
VERSION     DAOSDD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 20599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20599
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1109_length_20599_cov_4.771028"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..477
                     /locus_tag="PK733_06950"
     CDS             277..477
                     /locus_tag="PK733_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10315.1"
                     /translation="MYAFVIITNDLQKTITVGLADMIVDDLYAWGPLMGGAVISTLPV
                     VLLYMFASKYLVTGISLGGIKG"
     gene            507..1463
                     /locus_tag="PK733_06955"
     CDS             507..1463
                     /locus_tag="PK733_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ10316.1"
                     /translation="MKRVMMNLGTESKYDFFEARLKEKNYNVIRTSAYTPQEIIKAGH
                     GCEIAVIGAEDQWTKETFAGLKDFLKLIVRFGVGLDNIDINSATENGVMVANSAGANK
                     ESVAELAVALMLSCTRRIAWLENKLRSGTWKGLPRSRQFTGKTVGLVGFGAIAKCVAR
                     MVKGFNCKILAYDIFQDTASAKELGVAFCTLDELLEKSDFVSLHIPFNPQTYRLAAAD
                     FFKKMKNTAILINTSRGGVVNEDDLYDALVSGEIKAAGLDVFDKEPPDLDNKLFMLEN
                     IIITPHVASATEEAAGNIADICLQNIESYIDTGIPKIYVNRK"
     gene            1556..2464
                     /locus_tag="PK733_06960"
     CDS             1556..2464
                     /locus_tag="PK733_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ10317.1"
                     /translation="MNKTITGGVLPAFITPFSENGKIIDSAAKELIDYHLESGLDAFY
                     VGGSTGEAMIMSAEQRMHIAEVVVDYVADRAPVIVHVGSADTETAVKLAKHAEKIGAD
                     GISSVPPYYYKMSREYIKEFYRSIASSIKLPFLIYNIPILTGVSINSEFMIEMLEVEN
                     IVGLKYTDTNLEEFRKIKAYENGRIKAFMGYDAMLLSALVMGADGGIGSFYNIMPRAF
                     AGVYDNYKAGNFQEAREIQWHIDRYIAIIKKYICPANQPAIKSILKEMGINCGTTKPP
                     ILPLKDTVVEDMLDDLRSNGFFEFVK"
     gene            2555..3850
                     /locus_tag="PK733_06965"
     CDS             2555..3850
                     /locus_tag="PK733_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOJ10318.1"
                     /translation="MDISRIRELIGFYENELTNNILSFWLPRCIDRVNGGYFNCFDNE
                     GKQLISRDKYTWSQGRFVWMFSKLATMNCGTFNKAQREQFLTLAKVGRDFLEKHSLMC
                     EKDWRCVFLMDETGKPKYVDGYKKLDMSIYADCFVIAGFAKYAEAADDKDSYEFCKKL
                     YESAVSRVKTGEFSTLPYPLSPAYRAHGIPMIFSNVTREVYSAAEKFDKEYCLELQKN
                     LDFFTDDILTHFVDENNVLHEIITSDNRFVDNLLGQHANPGHTIEDMWFMIDAADILM
                     KPHYIPRVAAITKKAFEIGWDKEYGGLLHFCGVNGGEPVGSCQGVEDEPMLKQLIHGW
                     SDKLWWVHSEALYTSLLCYQRTGDDEFLDIYKKVFDYTYEKFPNPDREIREWIQILVR
                     DGRPQNKVVALPVKDPYHITRNYILIIELLYNMAKYYQA"
     gene            3902..5182
                     /gene="larA"
                     /locus_tag="PK733_06970"
     CDS             3902..5182
                     /gene="larA"
                     /locus_tag="PK733_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOJ10319.1"
                     /translation="MQISIPYGNSAKAVFAIPDQNYYGMLEPLQVTPADDPVLEIEKA
                     IDNPIGCPSLKDIVKYDRRVNIICDDISRPTPISLILPTLIKKLKEIGVSDSNVKIVM
                     ALGSHRYMTENEMRQRVGDDIYQNYKVVNSEFRKKENLIYLGTSRDGVEIHVSKEAMD
                     CDIRIGIGNIVPHPVMGWSGGGKILFPGVTGEKTVAQFHMQGGMSNENLYGREECLIR
                     LNMEEWVDIVGLHFVINTVLTPKMAIYKVVAGHYIKAHREGVKHAKVALGCKMRTNVD
                     IVVVSSYPADIDFWQSGKGMFSAEHALKDHNGTIVLVTPNYEGIGPHDEYPKYFGMDN
                     AEKILLRAYRGELVEGDPLAISVGTSMSKLRRRVNLVMVTDGVTEEEADICKIKHYPL
                     CRLQQAVDDAMAKYENPLVAAASHGGELFIYNGV"
     gene            5461..5664
                     /locus_tag="PK733_06975"
     CDS             5461..5664
                     /locus_tag="PK733_06975"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HOJ10320.1"
                     /translation="MTCGTVGILKLAKVKGYLDQLRPLLDRLIDNNFRIAQKLYYRKE
                     YGIKIDSKLNKGTTVIIVIPKEL"
     gene            5989..7209
                     /locus_tag="PK733_06980"
     CDS             5989..7209
                     /locus_tag="PK733_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ10321.1"
                     /translation="MSNIRPEGEYLVHLLSSVINDKQPQSPPVELNWEKLYKLSDYHK
                     VSNMVFYGVKRLNNDNKPPQSIMTKFDKDYKKGIWRETIQHTEAEQILKTFEENCILC
                     MPLKGYLIKYLYPQSNMRQMADIDILIMNEQTEQIKKLMSDTDMGFTLEREGENHDVY
                     YKKPFMNIEIHRRLVSENSPYSDYFHKVWDRAQLKDGCKFIYRLSNEDQFIYIMAHLA
                     KHYANGGTGIRSIMDIWIYNKHYSDRMDWKYIQSEFEKINLWEFAKTICGLSDVWFGN
                     KQSNEFYDHMAEFIFTCGVYGTRRYSIISSINKEISKCNGSNFKTLKTFKGAKIMYWL
                     QLFFLGQEHMKILYPFLETKTFLLPVCWMLRGIKCLLFKTKHTFQIIKSAHLVSEADL
                     SKLQSLHKKDGLIK"
     gene            7524..9728
                     /locus_tag="PK733_06985"
     CDS             7524..9728
                     /locus_tag="PK733_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOJ10322.1"
                     /translation="MKKSSSVNVLLTLLLLVFAGITASSFLYPGKVLASEISDFIYSE
                     VNENITITGYAGTETIVEIPSNIDGKPVKTIGYNAFREKSITSMTIPDSVTSIGNYAF
                     YKCSKLESAIIPESVTSIGNYAFYGCSSLAGITLPDSITSIGSSSFNMCSSLTSITIP
                     GSVTSISSNTFYGCSSLAEVVIREGTKVIGDSAFQGCGALTNVTIPETVTTIGVNTFY
                     GCTSLTDITIPESVSTIGKGAFSDCSKLARVTILGNVNSIGENTFYKCISLANITIPR
                     SVTSIVDKAFDGANPNLEISGYQGSYAETYAQDKSYKFNPLCLITIAQSQYGTATGSG
                     TYNYGQSVTLGAVPKPGYNFNGWYAGETKISSLPNYTIFVMDDITLTPDFIKMPASYL
                     EVTAVGGGSVTLNSETTSLPANYKCLHAKDAPIQLTATPDEGYVFAYWEDVRTSSIIS
                     TNSIYECIMGAGVSVKAVFYKNPTEASDAYTVVFKDISGKILQSANVAKGTEASPPPA
                     TPALTGYVFTGWDQDFSQVASNMIVSARYMRAPDAYTITVIGGEFSTGGTTGEFQFDM
                     LVTVKADTAPAGQKFSHWEQDGVKVSSDSTYRFFAPMKATALAAVFVDENAPIIETPF
                     IVLSNDVLMDTENKTMIFTANRYVPADYTLVESGMMLLNSNVPPADELTLETENALRG
                     RIKNDSTDQFYVRKSNINTGDTWYARAYLIYQDASGNIITVYSENTVNIVMK"
     gene            9749..9877
                     /locus_tag="PK733_06990"
     CDS             9749..9877
                     /locus_tag="PK733_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10323.1"
                     /translation="MESKTKKPYEKPEIEITEFEIEDITCSTPGMPGIYGGSFLDL"
     gene            9956..10675
                     /locus_tag="PK733_06995"
     CDS             9956..10675
                     /locus_tag="PK733_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10324.1"
                     /translation="MMQYRTRQYKVADLIVDMACSGQTLLEQGEPYLSNTGSHGDIAL
                     NINREKFEKNKAKHPELSLNELEYIFTGFAFSHALLDYNGFCLHSSAVAIDNKAILFS
                     APCGTGKSTHAGLWQKHFGRDRVVIINDDKPAIRLIDDAFYVYGTPWSGKTGLNMNLK
                     VPLQAVVFIEQAEKNHIRLLNNREAVQMLVYQSMRPNDDREKLSRLLALIDALLKKIP
                     VYKLGCTISIDAVRLVYETIL"
     gene            10809..11072
                     /locus_tag="PK733_07000"
     CDS             10809..11072
                     /locus_tag="PK733_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOJ10325.1"
                     /translation="MRIKSDYILREVAGNYVVVPVGKAAVNFNGMMTLNETGAFIWKE
                     LSVEISEEQLLAALLGKYDIDEATAKADISCFFEKLKAADVFE"
     gene            11065..11523
                     /locus_tag="PK733_07005"
     CDS             11065..11523
                     /locus_tag="PK733_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOJ10326.1"
                     /translation="MSRKVAMSELSPLMKEILENGGEVVFTVKGNSMTPLLRHGRDEV
                     CIVKPQDNILRKYDIPLFVRKDGKYILHRIVKVKPDGYVVMGDNQKTKEYPVLPSQVI
                     GVVKGVWRGGKYISCDNLWYRLYCRLWALGYPIRWVYLSWKSYSRKKHYV"
     gene            11872..12366
                     /locus_tag="PK733_07010"
     CDS             11872..12366
                     /locus_tag="PK733_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOJ10327.1"
                     /translation="MFCKTGSENVVAQSINAIDNTIKVVVPVRVLQEKISGIWEERER
                     LLIPGYVFIYVEDEVSFDLLKMLSSVYKILEYQTGERELIGSDYEYAEWIYRHNGRIG
                     PSRALVEGSTIKVIDGPLIDGIGTVTKLDRHKRRAWVEFDFDGKIQKVSLSVIDIDIC
                     SVSE"
     gene            13225..14469
                     /locus_tag="PK733_07015"
     CDS             13225..14469
                     /locus_tag="PK733_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOJ10328.1"
                     /translation="MDNNKLRNIPFSPPDITDEEIKEVIKAMKSGWITTGPRTKEFER
                     EIAEYIGINKAVCLNSATAAMELTLRILGIGPGDEVITSAYTYTASASVIDHVGAKIV
                     LVDTTPDFFEMDYEKLADAITEKTKAIIPVDIAGKMCDYDKIYKVVESKKELFKVNNE
                     IQGLFNRVIVMVDAAHAFGAKRKGQKCGQVADFTTFSFHAVKNLTTAEGGAVVWRNDL
                     GLDDEELYKQFMLYSLHGQSKDALAKNQKGAWEYDIIFPAYKCNMTDILAGFGLIQLK
                     RYEGLLERRKEIIEMYDSMLHPLGVLSLRHFGEDFSSTGHLYLARIPGISEDERNEII
                     IKMAEDGIATNVHYKPLPMFTAYKNLGFDIKDYSNSYRQYANEITLPLHTLLSDEDVV
                     YICGSLKKAMNGVINKKVAFAR"
     gene            14432..15217
                     /locus_tag="PK733_07020"
     CDS             14432..15217
                     /locus_tag="PK733_07020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ10329.1"
                     /translation="MGLLIRKSLLHDRGEKMLLKKWDDLPENMKNDRVRPYYEFLHKK
                     RLSLVAKRIFDVVVAILTFIILSPIFIVLCIAIKIDSKGPVMFRQIRITQYGKQFRIF
                     KFRTMVNNADKIGTQVTTKNDARITIVGKFLRKLRLDEIPQLFNIISGDMSFVGTRPE
                     VVKYVEKYTDEMMATLLLPAGVTSEASIQYKDEELLLANDEDADKTYIYEVLPEKMKY
                     NLRSIEKFSFWGDIKIMLRTISVVFRINTYSNDLAKDSEVINM"
     gene            15239..15850
                     /locus_tag="PK733_07025"
     CDS             15239..15850
                     /locus_tag="PK733_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10330.1"
                     /translation="MLETRVLSDLKYIDEIVDIHMRTFTGFFLTFLGKGFLRHLYKGF
                     IIHDKSNIIGAFDEGGNLMGFLAYSEDISQFYKQLLKKSLIPFAWYSFIAFLKKPKIM
                     FRLLRAFTYSESSKNEDLYIELSSIGVLPVAKNKGIGTMLISELKKIVNTNNFKYIKL
                     ETDKINNDSVNAFYLKNGFKLYKSYVTREGRNMNEYRYELNII"
     gene            16101..17249
                     /locus_tag="PK733_07030"
     CDS             16101..17249
                     /locus_tag="PK733_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ10331.1"
                     /translation="MKKRKKILICSVLSEAFYKSRRELIVEFIKMEYEVILVAPEQEN
                     FLQKEFNGMKINYYQVPIDRTGINPYKDLKTIAKLCEIIKIEKPEITYAFGGAKAAIY
                     TTIAASKRNVSRNFCMINGLGSIFRGSGIKSNIIKKIMSFLFKYSLAKSSGVLFQNRD
                     DLYEFVDNDLINESKCIIVNGSGVNLDKYPFTSPTVYNVFLFVGRLLGDKGIYEFIDA
                     AKIIKQKYPKTEFWILGGYDTNHTSVKENEIFSWVNSGLVKYFGRQDNVLPFYQKSSV
                     FVLPSYHEGTPRTCLEAMAVGRPVITTDAPGCKETVIDSVTGFLVPVKDYVNLSEKME
                     YFILNPQAISDMGNKAHMFVREKYDVNKVNKSIIDFLLFKQIKSSLNS"
     gene            18220..19344
                     /locus_tag="PK733_07035"
     CDS             18220..19344
                     /locus_tag="PK733_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOJ10332.1"
                     /translation="MSDPIKVLQIVTQMNRAGLESRLMDIYRNIDRSKVQFDFYTCRT
                     EKGFFDDEIVSLGGKVFYSEPLYFKQIFNVPKRFYSFLSIYSEYKIVHCHLNQWCGAV
                     LNGAEKANVPIRIAHSRTSLETKDLKNFIKNVIKFSVNKYATHRFAVSRKAGVWLYGK
                     KVFDSEVVEIWPNAIDCSKFKFEPTKREEYRKELNLLDSFTLIHVGNIRPEKNHKFLI
                     DVFKEIKKEQNNAKLLVVGSDYMSGEIHRYVEEHGVGKNVIFLGPRSDVSNLLQAGDV
                     FVFPSLYEGFPGAVLEAQASGLPCYISDTITEEVCVTNLVKRISLQKPIEYWKEQILN
                     NVNINRINTYDILKSSGYDIHSVSERYSFFYTNIINEIGE"
     gene            complement(19568..>20599)
                     /locus_tag="PK733_07040"
     CDS             complement(19568..>20599)
                     /locus_tag="PK733_07040"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ10333.1"
                     /translation="IQKFVDLALEGKIFESRPELLMQQIFAKIGIEPSAKEGLYGDTK
                     KLDVSGDGTCINTGASNFGNKVCDCIKNGIYNCDCARKFSDPYARWGWDSYHGQWFYG
                     HTEYILSVYNKDLKSDLPFYLRMVQAQRFDGISAVIALAEARKLYPDFNFNSFIGDGA
                     HDNYASYQLLHEWNMKAFIPLNETNKGNFQYPPHLNIDENGVPHCMAGHAMVNWGFNQ
                     DRCRIKYRCPLALGKIDSCDCKEQCSPSPYGRCIYTKPSWDLRLFTVVPRGSDEWKEH
                     MNSRTTSERVNKRILNDYGLEQSHTRGKKRTFWWSVVHSLNVLLYARLKKSKFSFIDL
                     LSERLTQAA"
BASE COUNT         7046 a         3051 c         4308 g         6194 t
ORIGIN      
        1 gccaacattt ttcccaaaga aaatatcgtt ggaaggttac aagaccctgt tctttagtac
       61 taaattcttt gtgcacatga agaacagtct gactatttca ttgattacta cgtttgtggc
      121 agtctttttc agtatgctgg ctgcttatgc aatttcacga ttccaattta gaggcaaaaa
      181 actgatttcg agatgtatca tctactctta cctgatgccc cggtcggtga tgtatatccc
      241 tttatacctg ctggttgtgt ttgctggaat gaatttttgt atgcatttgt aattataact
      301 aacgacctgc aaaagacgat aacagtggga ttggctgata tgattgtaga cgatttgtat
      361 gcatggggac ctctaatggg aggtgcggtg atttcaacac tgccggtggt attattatat
      421 atgtttgcat ccaaatatct ggttacgggg atttcactgg gcggtataaa gggataagtg
      481 agttattgtt gatgggaggg aaataaatga agcgtgtgat gatgaacctt ggaacggaat
      541 ccaagtacga tttttttgaa gcaagactga aagagaaaaa ctataatgtt attcgtactt
      601 ccgcttatac tccacaggaa attataaagg caggacatgg ctgtgagata gctgtcatag
      661 gtgcagaaga ccaatggacc aaagagacat ttgcaggact taaggatttt ttgaagttga
      721 ttgtacgttt tggagttggg ctggataata tagatatcaa tagtgcaact gaaaatggag
      781 tgatggtggc aaattctgca ggtgcaaata aggagtcggt tgcagagctt gcggttgctc
      841 ttatgctctc atgcaccaga agaattgcgt ggcttgaaaa taagcttagg tctggcacat
      901 ggaaagggtt gccacgctcc cgacaattta ctggaaagac agttggtctg gtaggatttg
      961 gtgcgatcgc caagtgtgtt gccagaatgg tcaaaggttt taactgtaaa atccttgcat
     1021 atgatatatt tcaggataca gcttcggcaa aggaactggg tgtggcgttc tgtacattgg
     1081 acgaactgct ggaaaaatcg gattttgtaa gtttacatat accgttcaat ccgcaaactt
     1141 atcggttagc agcagcggat ttcttcaaaa agatgaaaaa taccgccatt ctaattaaca
     1201 caagccgtgg tggtgtggta aacgaagacg atctatatga tgcattggta tctggtgaaa
     1261 taaaagctgc agggttggat gtgtttgata aagagcctcc ggatcttgac aataagttgt
     1321 ttatgcttga aaatataatt attacacctc atgtagcatc tgctactgaa gaggctgcag
     1381 gtaacatcgc cgatatatgt ttgcaaaaca tagaatctta tattgatact ggtattccaa
     1441 aaatatatgt caaccgtaaa taattaataa tatgtggcaa ccgcagtaat taataattta
     1501 tgtagtgtag accacatatt aaaaacgtat actactgacg aaaggatgaa ttatattgaa
     1561 caaaacaatt actggcggag tattacctgc atttattact ccgttctccg aaaatggaaa
     1621 gattattgac agtgcagcaa aagaattaat agactatcat cttgagagtg ggcttgatgc
     1681 tttttatgta gggggaagca ctggcgaggc gatgattatg agtgccgagc agagaatgca
     1741 tattgccgag gttgtagttg actatgtggc tgacagagct ccagtgattg ttcatgtggg
     1801 ttctgcagat acggaaactg ctgtcaaact ggcaaagcat gcggaaaaga ttggtgcaga
     1861 cggcatttcc tcagtaccac cttactacta taagatgagt agggaatata tcaaggaatt
     1921 ttataggtcg attgcatctt ccataaagct tccgtttttg atatataata ttccaatact
     1981 tactggtgtt tcaatcaata gcgaatttat gattgaaatg ctggaagttg aaaatattgt
     2041 aggcttaaaa tataccgaca caaaccttga ggaatttcgt aagatcaagg catatgaaaa
     2101 cggaaggata aaagctttca tgggatatga tgcaatgctg ctcagcgcac tggtgatggg
     2161 tgccgacggt ggtataggta gtttttacaa tattatgcct cgtgcttttg caggtgttta
     2221 tgacaattac aaagcaggca atttccaaga agccagggag attcagtggc atatagaccg
     2281 ttatattgct attattaaaa aatatatctg tccggcaaac caaccggcaa tcaagagtat
     2341 cctgaaggaa atgggcataa attgcgggac tacaaagccg ccaatcctgc cgttaaaaga
     2401 tactgttgta gaagatatgc ttgatgattt gcgcagcaat ggattcttcg aatttgtcaa
     2461 gtaagagcac agcattagat gcactcaaaa tagtataatg cttaagacat agctcttgag
     2521 taatctaagt gattaatgaa gtgaaggtga tacaatggat atttcaagaa tcagagaact
     2581 gattggattt tatgaaaatg aactgactaa taatatcttg tccttttggc tgccccgttg
     2641 tatcgacagg gttaacggag gctactttaa ctgttttgac aatgaaggca aacagcttat
     2701 aagccgtgat aagtatacct ggtcacaggg acgttttgtt tggatgtttt caaaacttgc
     2761 aacgatgaac tgtggtacat ttaataaagc acagagggaa caatttctta cgttagccaa
     2821 agtgggtcgg gatttcttag aaaagcatag cctcatgtgt gaaaaagact ggcgttgtgt
     2881 gtttttaatg gatgaaactg gtaaacccaa atatgtggat ggatataaaa aattggatat
     2941 gagcatctat gcagattgtt ttgtaatcgc aggttttgcc aaatatgcag aagctgcaga
     3001 tgataaggat tcctatgaat tctgcaaaaa gctgtatgaa tcggctgttt ccagagtgaa
     3061 aaccggtgaa ttcagtacac ttccatatcc gctctctcct gcatatagag cgcatggtat
     3121 accaatgatt ttctcaaatg taacacggga agtttatagt gctgcagaaa aatttgataa
     3181 ggaatactgc ctggagctgc aaaaaaatct tgacttcttc acagacgata ttttaaccca
     3241 ctttgtggat gaaaacaatg tactccatga aattatcaca tccgacaacc gttttgttga
     3301 taaccttctg ggacagcatg ccaatcccgg acatactatt gaggatatgt ggttcatgat
     3361 agacgctgca gatattttaa tgaagcctca ttatattcca agggttgctg ctattaccaa
     3421 aaaggctttt gaaatcggct gggacaagga atatggcgga ctgctccact tttgcggcgt
     3481 aaatggtggt gagcctgtag gtagttgtca aggtgtagaa gacgaaccta tgcttaaaca
     3541 actgattcat ggctggagtg ataagctttg gtgggttcac tccgaagcgc tgtatacatc
     3601 cttattatgc taccagagaa ccggagatga tgaatttctt gatatataca aaaaagtatt
     3661 tgactatacc tatgaaaagt ttccaaatcc ggatagggaa atcagagaat ggatacaaat
     3721 actggtaaga gatggtagac ctcagaacaa agtggttgca ctgccggtta aggatcccta
     3781 tcacataaca cgtaattaca tacttattat tgaactgctt tataatatgg caaaatatta
     3841 tcaggcataa atcaaaatta ttgaagaaaa atagtaattt gttgagatag gagacttaac
     3901 catgcagatt agtattccat atggaaacag tgctaaagca gtatttgcaa ttcctgacca
     3961 gaactattat ggtatgttgg agcctttaca ggtaacacct gcagatgatc cggttttaga
     4021 aattgaaaag gctatagata accccatagg atgtccgtcc ttgaaggata ttgtaaaata
     4081 tgaccgcagg gtgaatataa tttgtgacga tatttcacgt cctacgccaa ttagcctgat
     4141 tttaccgaca ctcatcaaaa agctaaagga aataggtgtc agtgacagca atgtaaaaat
     4201 tgtgatggca ctgggcagtc atcggtatat gacagaaaat gaaatgcggc agcgtgtcgg
     4261 tgatgacatt taccaaaatt ataaagtagt taattctgaa tttagaaaga aagaaaatct
     4321 aatttatctt ggtacttcac gggatggtgt agagattcat gtctctaaag aggcaatgga
     4381 ctgcgacatt cgaattggca taggaaatat tgtacctcac cctgtgatgg gctggtctgg
     4441 cggcgggaaa atcttatttc caggtgttac cggcgaaaag acagttgccc agtttcacat
     4501 gcagggtggt atgtccaatg aaaacctgta cggcagagag gaatgcttga ttcgcctgaa
     4561 tatggaagaa tgggtggata ttgtcggcct tcactttgtc ataaatactg tgttgacccc
     4621 taaaatggca atatacaagg tggtcgccgg gcattatata aaagcacatc gtgaaggtgt
     4681 caaacatgct aaagttgcct taggctgcaa aatgagaaca aatgtagata ttgttgttgt
     4741 cagttcttac ccggcagata ttgatttttg gcagtctggt aagggtatgt tttcagcaga
     4801 gcatgctctt aaagatcata atggtaccat tgttctcgtc actccaaatt atgaaggtat
     4861 aggtccccat gatgaatatc ccaaatattt tgggatggac aacgcagaaa aaatactttt
     4921 acgtgcatac aggggagaac tagttgaggg agatcccctc gcaatatcag ttggtacctc
     4981 aatgtcaaag ctgcgcagga gggtaaacct tgtgatggtg acagatggag ttactgaaga
     5041 agaagctgat atatgtaaaa tcaagcatta tcctttatgt agactccagc aagctgtaga
     5101 tgatgcaatg gcaaaatacg agaatcccct ggtagcagca gcgagtcatg gtggggagct
     5161 ctttatctac aatggtgtgt gaagccaaag attcataaga ggtaattaat aggaaacagc
     5221 gagcctattc tcaatttaat ggaatagatg aaagctgtgg cttaggtacg aaacttcact
     5281 tgcttatttt gattgaatcc gcttgattgt acgtgctata atatacttgt aggcaaataa
     5341 atcattgaca agttttatgg atatattaaa aaaggttaat atcccatggt gtgaatatac
     5401 agaagattta gaaaatgcag atgatctcgc agtaaatctg atattacagg aatcggagag
     5461 ttgacatgtg gaacagttgg tattctcaaa ctggcgaaag ttaaaggata tctagatcag
     5521 ttaagacctt tattggatag actgatagat aataatttca ggattgctca aaaattatat
     5581 tatagaaaag aatatggaat caaaattgat agtaagctta ataagggtac tactgtaatt
     5641 attgttatac ctaaggaact ttaattatta agcataagtt aaaatttcga caaatttcta
     5701 caatattgtc taccgttagg agatgtttcg tttagcgaat gggatatgaa gccttatgag
     5761 atgtagatga attgtatcaa taacattgag atgataaatt tatttttatc tatgcaaatt
     5821 tctggtatta taatacaagt taacatgctg tagctattat tatatctgct gcagctattg
     5881 atatggaaaa ttttatgttg tatgattgta ttatacttca aaattctaca taatttgcac
     5941 gaaataattt ataaaataac ttacacaatg caaaaagggg tgtagtgatt gagcaacatc
     6001 cgacccgaag gggaatattt agtacatttg ctttcttcag taattaatga taagcagccg
     6061 caaagcccac cggtggaact taattgggaa aagctttaca agctttcaga ttatcataaa
     6121 gtatccaata tggttttcta tggagtaaag agattaaata atgacaataa accaccacaa
     6181 agtattatga cgaaatttga taaagactat aaaaagggta tttggaggga aaccatacag
     6241 cataccgaag cggagcagat tttaaaaacc tttgaagaaa actgcatttt atgtatgcct
     6301 ttaaaaggat acctgattaa atatctatac ccgcaatcca atatgaggca gatggctgat
     6361 attgacattc tgattatgaa tgagcaaaca gagcaaataa agaagcttat gtcagataca
     6421 gatatgggat ttactctgga gcgtgaaggc gaaaatcatg atgtatatta taagaaaccc
     6481 tttatgaata ttgaaataca ccgaagactt gtatcagaaa actcgccata tagtgattat
     6541 ttccataaag tatgggacag agcccaattg aaagatggct gcaaattcat ataccggctt
     6601 tccaatgaag atcaattcat ttatattatg gcacatcttg caaagcacta tgccaatgga
     6661 ggaacaggca tccggtccat aatggatata tggatatata ataaacatta ttccgatcgg
     6721 atggattgga agtacataca aagtgagttt gaaaaaataa atctttggga gtttgcaaaa
     6781 accatttgtg gattgagtga tgtatggttt ggaaacaagc agagcaatga gttttatgac
     6841 catatggcag aattcatatt tacctgtgga gtttatggta caagaagata ttcaataatt
     6901 tcctccatta acaaagaaat aagtaagtgt aatggtagca actttaaaac cttaaaaacc
     6961 tttaaaggtg ctaaaattat gtattggcta cagttatttt tcctgggaca ggagcatatg
     7021 aaaattttat atccgtttct tgaaacaaag acatttttat tgcctgtgtg ctggatgctg
     7081 cgaggtatta aatgcttgct gttcaagacg aaacatactt ttcagataat aaaaagtgcc
     7141 catttggttt ccgaagctga tttatccaag ttacaaagtt tacataaaaa agatggcctt
     7201 ataaaataga tttaatgtgg gtgcataacc aatgctttct tcacttaaat catatgcacc
     7261 cattcaatgt ggttttttca atactgtaat aatttctctg gcttttcttg tcgaataatg
     7321 caaaatattg tctaacgatt tttagcgagt tatagaggat tttgcaactc tatgtagaat
     7381 taaatatcaa aagtagaata aaaagttatt gtataatcag gctatataaa acctgaaaat
     7441 gacagcagag taatgggaca aattttctca aaagatagtt tactccatta cctgacttct
     7501 aaaaagcttg gaggaattaa aaaatgaaaa aaagcagcag cgtaaatgtt ttattaaccc
     7561 tattattact cgtttttgcc ggtattacag cgtcaagctt cttatatccc gggaaagttt
     7621 tggcttcaga aattagtgat tttatatact ctgaggttaa tgaaaatatt acaatcacag
     7681 gctacgcagg aactgaaacc attgtagaaa taccttcaaa tattgatgga aaacctgtta
     7741 agacaattgg ttataatgcg tttagggaaa aaagcataac aagcatgaca attcctgaca
     7801 gtgtaacaag tataggtaac tatgcctttt ataagtgcag caagctggaa agtgcaataa
     7861 tccctgaaag tgtaaccagc ataggcaact acgcatttta tggttgcagc tcattggcag
     7921 gtataacact ccctgacagt ataaccagta ttggcagctc ttcttttaat atgtgcagct
     7981 cactaaccag tataacaatc ccgggaagtg tcacaagcat aagcagcaat accttctacg
     8041 gctgcagttc actggcagag gtagtaatcc gggaaggaac aaaggttata ggagattctg
     8101 cttttcaggg atgcggggct ctcaccaacg taacaattcc agaaaccgta acaaccatag
     8161 gtgttaacac tttctacggt tgtacttcct taacagatat aacaattcca gaaagtgtat
     8221 caactatagg aaaaggcgcg ttttcagact gcagtaaatt ggcacgcgta acaatactgg
     8281 ggaatgtaaa cagtatagga gaaaatactt tctacaagtg catatcattg gccaatataa
     8341 caattccccg aagtgtcaca agcatagtag ataaagcttt tgatggagca aacccgaatc
     8401 ttgaaatatc cggataccaa ggttcctatg ctgaaacata cgcccaggat aaaagttaca
     8461 aattcaatcc actttgccta ataacaatag cacaatcaca atatggtact gcaaccggta
     8521 gtggtacata caactatggc caaagtgtaa cccttggggc cgtaccaaag ccgggatata
     8581 acttcaatgg ctggtatgca ggtgagacaa agatatcctc cctgccaaac tatacaatat
     8641 ttgtaatgga tgatattaca cttacgcctg attttattaa gatgcctgcc agttatctgg
     8701 aggttacagc agtaggtggt ggttctgtaa cactaaacag cgaaaccaca agccttcctg
     8761 caaattacaa atgtttgcat gcaaaagatg ctccaattca attgacagcc actccggatg
     8821 aaggatatgt ttttgcatac tgggaagatg taagaacttc aagcatcatt tctaccaatt
     8881 caatttacga atgtattatg ggtgccggag taagtgtaaa agcagtgttc tacaaaaatc
     8941 caacagaagc atctgacgca tatacagttg tattcaagga cataagcggg aaaatactgc
     9001 aatcggcaaa tgttgcaaaa ggtacggaag caagccctcc gcccgcaacc cctgctttaa
     9061 ccggatatgt ctttaccggc tgggatcagg atttttcaca agtagcatcc aatatgatag
     9121 ttagtgccag atatatgaga gcaccggatg cttataccat aactgttata ggcggggaat
     9181 tttctaccgg aggaacaaca ggcgaatttc agtttgacat gcttgtaaca gtaaaagccg
     9241 atactgcccc cgcaggacag aagttcagcc attgggaaca ggatggtgtg aaagtaagca
     9301 gcgacagcac ataccggttc tttgccccta tgaaagcaac cgctcttgcc gctgtatttg
     9361 tggatgaaaa tgcccctata attgaaacac cgtttattgt gttatccaat gatgtattaa
     9421 tggacactga gaacaaaaca atgatattta cagctaacag atatgtgccg gccgattata
     9481 cactggttga aagcggtatg atgctgctta actcaaatgt gcctcctgct gatgaactta
     9541 ctctggaaac agaaaatgca ctccgcggga ggattaaaaa tgactccacc gaccagttct
     9601 atgtaagaaa gtccaacatt aatacaggtg acacctggta tgccagagcc tatttgattt
     9661 atcaggacgc cagtggaaat attattacgg tatatagtga aaatactgtg aacatagtaa
     9721 tgaaatagaa cgaaaggagg cccataaaat ggaaagtaaa acaaaaaagc cctatgaaaa
     9781 accggagatt gaaattaccg agtttgaaat tgaggatatt acctgcagca cacccggcat
     9841 gcccggcata tacgggggaa gtttcttaga cctgtaatgc atataagaat aatagtactg
     9901 tatgccaatt tggaaatata gcaaagtact aatagtataa ggtgacaaat acagcatgat
     9961 gcaatataga acaagacagt ataaggtagc ggatctgata gttgatatgg catgctccgg
    10021 ccaaacatta ttagagcagg gagaaccata tttgagtaat accggcagtc atggagacat
    10081 tgcactgaat attaaccgag aaaagtttga gaaaaacaag gcaaaacacc ctgaattatc
    10141 tcttaacgaa ttggaataca tctttacagg ctttgctttt tctcatgctc tattggatta
    10201 caacggtttc tgcctgcatt cgtcggcagt tgcaatagac aataaagcca tattgttttc
    10261 agctccctgt ggtacgggta aatccaccca tgcagggctg tggcaaaagc atttcggcag
    10321 agacagggtt gtcataatca acgatgataa acctgcaatc cgcctgattg atgatgcctt
    10381 ttatgtatat ggcacaccat ggagtgggaa aaccggcctg aacatgaatc tgaaagtgcc
    10441 attgcaggca gttgtgttta ttgagcaggc ggagaaaaac catataaggc ttctaaacaa
    10501 cagagaagca gtacagatgc ttgtttatca gagtatgcgc cccaatgatg accgggagaa
    10561 attgagcagg cttttagcac tcattgatgc tttactcaaa aagatacctg tgtacaaact
    10621 gggatgcaca attagtatag acgcagtaag actggtttat gaaacaattt tatagggaca
    10681 catttttagt ttttgaagaa atatattcca gaagttatta ttacggtgct tagagcctca
    10741 acttatgctt cttccaataa ttccttcaaa atattatgtg tccctctttt ggaaaggagg
    10801 atatagaaat gagaatcaaa tctgattaca tactaagaga ggttgcagga aattatgtgg
    10861 ttgttcctgt gggaaaggca gcggtaaatt tcaatggtat gatgactttg aatgaaacag
    10921 gagcgtttat atggaaggag cttagtgttg agataagtga agagcagctg cttgcagctc
    10981 tgctgggaaa atatgatata gatgaggcaa ctgcgaaagc agatatttcc tgtttctttg
    11041 agaaacttaa ggcggctgat gtgtttgagt agaaaagtag ccatgagtga gctttcaccc
    11101 ttaatgaaag agatccttga aaacggcggg gaagttgttt ttacagtaaa gggaaacagc
    11161 atgactccgc tgcttcgcca cggcagagat gaagtatgta tagtgaagcc acaggataat
    11221 attttaagga agtatgatat tcccctgttt gtacgaaaag atggaaagta catcctgcat
    11281 cgcattgtta aagtaaagcc ggacgggtat gtggtaatgg gtgataacca aaagacaaaa
    11341 gaatatcctg ttttaccttc acaggtaatt ggtgtagtca agggtgtttg gcggggagga
    11401 aaatatattt catgtgataa tttatggtac cgtctatact gcagactttg ggctttaggg
    11461 tatcctatca gatgggttta tttaagctgg aaaagttata gtagaaagaa acattatgta
    11521 taattttaac atccgacata tttcaacaaa ttatgacata taggctgtag tataatactg
    11581 caaaacatat aaactattaa tattccataa aaaacaggat tttattcgaa atattaatag
    11641 aaatgtgcaa attcatctat taattttaga aaaggggtgt tgacataaaa aagcaagtat
    11701 tataaacttt aatagtagtt catcatgtga acttcttaat ttatggaaaa tattggacaa
    11761 acttcagggg ataaagtata taaatttgct aaatagaatt gaacattttt gaaatgttct
    11821 ttatttaata ggatacggaa aggtgattga atttttttgg aagcctactg tgtgttttgc
    11881 aaaacgggct cggagaatgt tgttgcacaa agcataaacg caatagataa tacgattaaa
    11941 gttgtagtgc ctgtccgtgt tttgcaggag aaaatcagtg gaatatggga agaacgagaa
    12001 cgtttgttga ttccgggata tgtttttatt tatgtagaag atgaggtttc atttgatctt
    12061 ttgaaaatgc tatcaagcgt ttataaaatc ctggagtatc aaacaggaga aagggagctt
    12121 ataggctcag actatgaata tgcagaatgg atatacaggc ataatgggag aatcggaccg
    12181 tcaagggcat tagtggaagg cagtaccata aaggttattg atggcccgct aattgacggt
    12241 ataggaacag taaccaagct ggaccgccat aaacgccgtg cctgggttga gtttgacttt
    12301 gatggaaaaa tacaaaaggt atcattaagt gtaatagata tagatatatg ctccgtatct
    12361 gagtgaataa cagagagatg gggcataagc tttcttataa agagaaatag aacaataagt
    12421 gacggtattc tttaaaacat cctattaacg ttgatcatcg actcgtgcgt gtcaaaagtt
    12481 gtaggactgc atagaaaagt tcaggtagtg taaaggggga ttttacgcga aagggcgaag
    12541 ctgtgcgggg gatgcgggaa ttagtgacaa tattaatggt taaaaggtgt acaagctata
    12601 agggtggcag gacttagagc aagagcatta tagtcaagtg actatagaga gtggcagtat
    12661 atatagccca gatacgctat aacgcttgaa atagaatgag tacatgtaat tctaccataa
    12721 atagaatgtt taaaacttaa atagtggatt tagtttagtc gaaatcatac agaagatgta
    12781 tttgtccagt ttataaaaga ttatggcgga tggatttgtc ctagtaatgg tgatatctat
    12841 tatgaaaatg gaaagattta gtgtaatatt catgcaaggg ataaagatga tgagagtgat
    12901 gatgaggacg acgaggatgt tccatttcta tgatcttgat actattgaat taacatagct
    12961 attctcggac tgttaagtga ttgaatggta gtgccgtcgt gagtataggg aaagaataac
    13021 aagggtgcaa atgtagtaat agcaagggtt tcataggttg agtatgtagt cggaatatta
    13081 cagaatggtt acaaggttga ccattcaatc atgaagaatg gttaaagata ctgaaagcgt
    13141 tatacataag gaagttcaga agattgaaaa gtaattatac atatttctag atagataatt
    13201 cgtagaaaca aggggggact caaaatagat aataataaat taagaaacat tccattctct
    13261 ccaccggata taacggatga agagattaaa gaagttataa aagcaatgaa gtctggctgg
    13321 attacaactg gtcctagaac aaaagaattt gaaagagaaa tagctgaata tataggtata
    13381 aataaagcag tatgtttaaa ctctgcaaca gctgctatgg aattaaccct tcgaattctg
    13441 ggaataggac ctggagatga agttattact tcagcttata catatactgc ttcagcttct
    13501 gttatagatc atgtgggagc taagatcgtt ttggttgata cgactcccga tttctttgag
    13561 atggattatg aaaaattagc tgatgcaatt actgagaaaa caaaagctat cattcctgtt
    13621 gatatagcag gtaagatgtg cgattacgac aagatatata aagtagttga aagcaagaag
    13681 gaattattca aagttaataa tgagattcaa ggtttattta atagggtaat tgtaatggta
    13741 gatgcagctc atgcttttgg agctaaaaga aaaggacaaa aatgtggaca agtagcagac
    13801 tttacaactt tttcttttca tgcagtaaaa aaccttacaa cagctgaagg tggggccgtt
    13861 gtttggagaa atgacttagg attagatgac gaagaattgt acaagcagtt catgttatat
    13921 agtcttcatg gtcagtcaaa ggatgcatta gcaaagaatc aaaagggtgc atgggaatat
    13981 gatattattt ttccagccta taagtgcaat atgactgata tcttagccgg gtttggattg
    14041 atacaactta aaagatatga aggcttgttg gaaagacgta aagagattat cgagatgtat
    14101 gattcaatgt tgcacccatt aggtgttcta agcttacgtc attttggaga agatttttct
    14161 tctacaggcc atttatattt agctagaata cctggtatta gtgaagatga gagaaatgag
    14221 attattataa agatggctga agatggtatt gctacaaatg tacattacaa gccacttccg
    14281 atgtttacag catataagaa tttagggttt gacattaagg attattcaaa ttcttatagg
    14341 cagtatgcta atgagattac acttcctctt catactttgt taagtgatga ggatgtggtg
    14401 tatatctgtg gaagcttgaa gaaggcaatg aatggggtta ttaataagaa agtcgctttt
    14461 gcacgataga ggtgaaaaga tgctattaaa aaagtgggat gatttaccgg aaaatatgaa
    14521 gaatgataga gtaaggcctt attatgaatt tttgcacaag aaacgtctta gcttagttgc
    14581 taaacgtata tttgatgtag ttgttgcaat tttaactttc attattcttt cgcctatatt
    14641 tatcgtttta tgtattgcta tcaagataga ctcaaaagga cctgttatgt ttcgacagat
    14701 tagaataact cagtatggaa aacagttcag gatttttaag tttagaacaa tggttaataa
    14761 tgcagataaa attggtaccc aagtaaccac aaagaatgat gctagaataa ctatagtcgg
    14821 taagttttta agaaagctaa ggcttgatga aatcccacag ctatttaaca taatttctgg
    14881 tgatatgagc tttgttggta caagacctga ggttgtaaag tatgttgaaa aatatacaga
    14941 tgagatgatg gctacattgc ttttgccagc tggagttacc tcagaagcaa gtattcagta
    15001 taaggatgaa gagttattat tagctaatga tgaggatgct gataagacat atatatatga
    15061 agtactacct gaaaaaatga aatacaatct tagaagtata gaaaagttta gcttttgggg
    15121 agatattaag ataatgctta ggactatatc ggtagtattt aggataaata catattctaa
    15181 tgatttagca aaagatagtg aagtaataaa tatgtagagt taaagattgg ggttatatat
    15241 gttggaaaca agagttttat ctgatttaaa atacattgac gagattgtag atattcatat
    15301 gcggacgttt acagggtttt ttctaacttt cttaggtaaa ggtttcttga ggcatttgta
    15361 taaaggtttt attatacatg ataaatcaaa tataattggt gcatttgatg agggtggtaa
    15421 ccttatggga tttctagctt attcagaaga tattagtcag ttttacaaac aactattaaa
    15481 aaagagcctt attccttttg catggtatag ttttattgct tttttaaaaa aacctaaaat
    15541 tatgtttaga ttgctaagag cttttacgta ttctgagagt agtaaaaatg aagatcttta
    15601 tattgaactt tcctcaattg gagtattacc agtagccaaa aataaaggaa ttggcacaat
    15661 gcttattagt gagttaaaaa aaatagtaaa tacaaataac ttcaagtaca ttaagctaga
    15721 aacagataag attaataatg attctgttaa tgcgttttat ttaaaaaatg gttttaaact
    15781 ttacaaatct tatgttaccc gtgaaggaag aaatatgaat gaatataggt atgagctgaa
    15841 tatcatttag actatggaga tggatagcat tagaagaaag tgaaaaagta tattaatatt
    15901 aatgtaatta ttttgaactg tgctaattgt gaatcaagat aaaaaatcaa ggtaggagtt
    15961 gataactttt atcatagagt tcgacataaa tatacagaat gtatcatgtt tattatttgt
    16021 tatgcattgg attgtagagg ggccacatat atattatatt taagctatag catttatgat
    16081 tttatggagt gtggagtaaa atgaagaaaa gaaaaaaaat ccttatatgt tcggtgttat
    16141 ctgaagcatt ttataaatct agacgcgaat taatagtcga atttataaag atggaatatg
    16201 aagttatact cgtagcacca gaacaagaaa attttttaca aaaagaattt aatgggatga
    16261 agattaacta ttatcaagta cccatagata ggactggaat taatccttat aaagatctta
    16321 agactattgc taagctttgt gagatcataa aaatagaaaa accagaaata acatacgcat
    16381 ttggaggtgc taaggcagct atctatacaa ctattgctgc atctaaaaga aatgtaagta
    16441 ggaatttttg tatgataaat ggactaggtt cgatattccg tggaagcggc attaaaagca
    16501 atattataaa aaaaataatg agttttctat ttaaatactc attagcgaaa tctagtggcg
    16561 ttttatttca aaatagggat gatttatatg aatttgttga taatgatcta attaatgaaa
    16621 gtaaatgcat tatagttaat ggctcaggag taaaccttga taaatatccc tttacttcac
    16681 ctacagtata taatgtattt ttatttgtag gtagattatt aggagataaa ggaatatacg
    16741 aattcattga tgctgccaaa attataaaac agaaatatcc aaaaacagaa ttctggattt
    16801 taggaggata cgatactaac cacacttctg taaaagagaa tgaaatattt tcttgggtta
    16861 attctggatt agtaaaatat tttgggcggc aagataatgt gcttcctttc tatcaaaaat
    16921 cttctgtttt tgtattacct tcttatcatg aaggaacccc gagaacatgt ttagaggcga
    16981 tggcagtagg tagacccgtg ataacaacag atgcaccagg ttgtaaagaa actgtaattg
    17041 atagtgtaac aggatttctg gttcctgtaa aagactatgt aaatctttct gaaaaaatgg
    17101 aatatttcat tttaaatcca caagctattt cagatatggg taataaggca catatgtttg
    17161 tgagagaaaa atatgatgta aataaggtta ataaatctat tattgatttc ctattattta
    17221 aacagataaa aagtagccta aacagttagg attacacctt taataattaa tattatagat
    17281 atttgtagta tcaaataaat gatgaaaact gattaagaat gaaagtattt aattgtatta
    17341 atttagacat gtgtatggtc gattacgata gaatagatta tgtactataa tatgatggaa
    17401 tttactagac agaaagaagc taaaagtttg tttatgtatt aagttagtta gctagcatat
    17461 accgacgggt aaggtttacc ctaaaaagag gataaagagg gaatatgata aaaacctact
    17521 taactaaaat agttaatgaa gaatgctaaa agttctttat tattattgcc gattagtaaa
    17581 ttacataata ggaaattttc taattgtatg caaattactt aaatacttgt aactaaattt
    17641 caaatgatag tcaagggtat aaaggcttga ctagtatgat ttccttgact cttgcctgaa
    17701 atttgttgaa aaaaggttat gataacgcta gaaaatcgct caattttaat taaattttac
    17761 attatcaagc aatataagac tgatgtctat gatttaagat actttaatgc agttggaaaa
    17821 tattaattat gccatacgct gttgcaactc catggtttgt tatacattaa gtagttgcta
    17881 tataactaac agcaaacaat caacgaggga gcgaagagag tcatagtttt tcatacatca
    17941 agaatgttat caatggtacc cttacaattt gtcttacaat tttcgaagca attgaatggt
    18001 cgttaaagtt gcatataatg aaaacaagtt ttattctaca tataacgcag cgtgaaaaag
    18061 agttatggat tgaatatcaa gctgatattt aaatgagata tagaaaatat taagcactca
    18121 cattctaaaa taagcaaagt ttgaaatact tagttatagt cctgatttta ttagaaaatg
    18181 gagttagtta acgatagaat agaataagga gaaatttgaa tgtctgatcc aataaaggtt
    18241 ttgcaaattg ttacacagat gaatagagcc ggacttgaaa gtcgtcttat ggatatttat
    18301 agaaatattg atagaagtaa agtacagttt gatttttata cttgtcggac tgaaaaagga
    18361 ttctttgatg atgaaattgt ttctttaggt ggtaaagtat tctattcgga accattgtat
    18421 tttaaacaaa tatttaatgt accaaaaagg ttctatagtt ttttatccat ttacagtgaa
    18481 tataaaattg ttcattgtca tcttaaccaa tggtgtggtg ctgtattaaa tggtgcagaa
    18541 aaggctaatg ttcctataag aattgcacat tctagaactt cacttgaaac aaaagattta
    18601 aaaaatttca ttaaaaatgt tattaagttt tctgttaata agtatgctac tcataggttt
    18661 gcggtttcta gaaaagcagg agtttggtta tatggaaaga aagtatttga tagtgaggtt
    18721 gttgaaattt ggcctaatgc aattgattgt tctaaattta aatttgaacc tactaagaga
    18781 gaagaatata gaaaagaact taatttactt gattcattta cgctaataca cgttggaaac
    18841 attcgtcctg aaaaaaatca taaattttta attgatgttt tcaaggaaat aaagaaagaa
    18901 caaaataatg ctaaactttt agtagttggt tcagattata tgtcaggaga gatacataga
    18961 tatgtagaag aacacggagt aggcaaaaat gtgatttttt taggccctcg ttctgatgtt
    19021 tcaaacttgc ttcaagcggg ggatgtattt gtattcccat ctttatacga aggtttccca
    19081 ggagcagtgt tagaagctca agcatctgga ttaccgtgct atatttctga tactattact
    19141 gaagaagtct gtgttacaaa tttagttaaa cgaatttcat tacaaaagcc aattgagtat
    19201 tggaaagaac agattttgaa taatgtcaat attaatagaa taaatactta tgatattctc
    19261 aagtctagtg gatatgatat tcattcagtt tctgaacgat atagtttctt ttacactaat
    19321 attataaatg aaataggtga ataaatatga acattaaaat aaatgcagct attgagtgat
    19381 ttcgaaaact aatttccagt aaataaatgg gagttaaaaa tcactaaaat tgagtaggta
    19441 tcaataaaaa ataagatata ttttagaaaa tgtagtaaat tagcatgttt gaaccttgaa
    19501 aagagaatag caaagctaac cttacagaaa taaggcttta aaaaggtgta gaataaatta
    19561 attttactta tgcagcttga gtaagtcttt cactaagtaa atcaataaag ctaaattttg
    19621 actttttaag tctggcatac aaaaggacat ttagtgaatg tactacactc caccagaaag
    19681 tacgtttctt acctctggta tgagactgct caagcccata atcattaagt atacgcttgt
    19741 ttacacgttc agaagtggtg cggctgttca tatgttcttt ccactcatca gagccgcgag
    19801 gaactacagt aaacaaacga agatcccaag aaggctttgt ataaatgcat cttccgtatg
    19861 gtgaaggaga acattgctct ttacaatcgc aggaatcaat tttcccaagt gctaaagggc
    19921 aacgatattt aattctgcat cggtcttggt taaatcccca atttaccatt gcatgacctg
    19981 ccatacagtg aggaacacca ttttcatcaa tatttagatg tggcgggtac tggaaattac
    20041 ctttattggt ttcatttaaa gggataaaag ctttcatgtt ccattcatga agcaattggt
    20101 atgaagcata attgtcatgg gcaccatcac cgataaagct gttaaaatta aaatcaggat
    20161 aaagttttct ggcttcggca agtgcaataa ccgctgaaat gccgtcaaac ctttgagctt
    20221 gaaccattct gagataaaag ggaaggtctg attttaagtc cttattgtaa actgacagaa
    20281 tgtattcggt atggccataa aaccattgac catgatagct atcccaaccc caacgggcat
    20341 aagggtcgga gaacttacgg gcacagtcac agttataaat accatttttt atacaatcac
    20401 aaaccttatt gccaaagtta ctggcaccgg tatttatgca cgttccatca cctgaaacat
    20461 caagtttttt agtatctccg tataaacctt ctttggctga gggctctatc ccaattttgg
    20521 caaaaatttg ttgcatgagc aattcgggtc ttgattcgaa gatcttaccc tctagagcta
    20581 agtcaacaaa tttctgtat
//
LOCUS       DAOSDD010000057        20509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1113_length_20509_cov_5.509827, whole genome shotgun sequence.
ACCESSION   DAOSDD010000057 DAOSDD010000000
VERSION     DAOSDD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 20509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20509
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_20509_cov_5.509827"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..542
                     /locus_tag="PK733_07045"
     CDS             234..542
                     /locus_tag="PK733_07045"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ10334.1"
                     /translation="MIPYYEEIENIKNQILSKYNPKDIILFGSCAKGRVSRTSDIDIC
                     VILDTTDKRQIVREMLVDIESDADLDIVVYTPDEWEKYKYDKAKFANIINRTGVSIIG
                     "
     gene            535..921
                     /locus_tag="PK733_07050"
     CDS             535..921
                     /locus_tag="PK733_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOJ10335.1"
                     /translation="MVDSTKCNEWFVMAQKDIKGAKILFENDADNEIVCFHCQQAVDK
                     YLKGYLIYITGELQEGHSLVKLCKKIMKYDKTFGEFLKDMAFINTFYIETRYPAADPL
                     IVTKEDTEECFKITQNVISKINSLIS"
     gene            1006..1791
                     /locus_tag="PK733_07055"
     CDS             1006..1791
                     /locus_tag="PK733_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HOJ10336.1"
                     /translation="MGNKNVDKGKIYKTFEKDLYAPVRSYLMQQGWDVKGEVKYCDVA
                     AVKDDVFLVVEMKTSLNLDVILQAAIRQRTADIVYIAVPKNSRILFTKRWKNILYLLR
                     RLEVGLLLVDLKFQSFADEKVQHNEAGVSKARVEEALKPEPFNRNMSRKSSGKKRDLI
                     LKEFGARYGDYNTGGVTGEKLVTAYRELALHIAALLDKNEKLSIKELKALGTDNKKTA
                     KLLQDNFYGWFQRVSRGVYELSENGKEEIEEYREIMEHYLKKC"
     gene            complement(1925..3595)
                     /locus_tag="PK733_07060"
     CDS             complement(1925..3595)
                     /locus_tag="PK733_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10337.1"
                     /translation="MKKFKSLAIVLVIISLLLTSVGCVIKDSGGQTSNSNKVADTGNK
                     DEKGSEAKQPRKLHVLGPDKSGKVVKFGDREKFPVWKEVQKLLDQENLEIKYEIVPFE
                     QYNVVIQTRMAAAGDLPDIVNLSPLDDTTVLNLAKQNTILELNSLIDKYSNGNIKNMY
                     TKDFPFAKQLTTSPDGKMYWFSNLHIKLYKGTQPAPVALTMIIRKDWLDKLNIPAPTT
                     AQEYLNALKTMRKQDANASGKEDEILIYDPSVFKGAIAQWFGLGTDITAVDIENKKVV
                     SPWYQEGIKEYFKYVQTLVKEEILDTSLIGAGSEAVQQKITENKAASLSDYNMETYRE
                     PTIQGGGEYTPLMPLKAVEGIKPAAQKEPPFLVWKKYAITKNCKNPDAAISFFDVIYS
                     KEYADLLFWGIKDFTYKVNDDGIREFVDITGSFDTKLADSGKIAGNQVFGDTVFPRVQ
                     FANLEYELANIPQHKKDHQLKIIDYSPYYVNQNQNYLAIPNTEQLERKTKVLNDLKTY
                     SAELAAKLALGQKSLDDWDVYITELKKLGLDQLIEMDQQLLDRYFELK"
     gene            complement(3733..4680)
                     /locus_tag="PK733_07065"
     CDS             complement(3733..4680)
                     /locus_tag="PK733_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10338.1"
                     /translation="MVDNSINFNIKSYKNPCSKNSGRIKEGTSFANFMLYFFAVLICI
                     VTLYPFYYVVIMSISSPIKAASMDVYLYPKGLTLGSYRLIVRDSKMWWAYMNTLIYVI
                     CGTFLTLLTSILGAYPLSVRNLAGRKWLVSYLIIPMYFGGGLIPTFLLINKLGLYNTR
                     WAIIIPGAVSIWYIILVRTYFITIPDSMKESAFIDGANHFQLLFKIVVPVSKPILAVI
                     SIYTIVGIWNSWFSALVYLPNEMLHPLQMYLYRVLVQQTVDLKELAFDEMAEAAEMIF
                     TNMQLKYSIIVFTTLPIIFTYPFFQKYFIKGVMLGSLKG"
     gene            complement(4808..5767)
                     /locus_tag="PK733_07070"
     CDS             complement(4808..5767)
                     /locus_tag="PK733_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10339.1"
                     /translation="MVNSILYYTKNKIIVTPSKHRHIQENIQLYLIMLPVLLHIFIFH
                     YIPMYGVAIAFQDYYPGSPFIALDGSVSWVGLKHFVKFFSGPYFGRLFTNTFSLSLYI
                     LIFGFWVPIVFALLINELGNGFLKKFVQTASYLPYFISTVVVAGMVLSFINTDGVINS
                     IIKSLGKKAIPFNTEPGYFPAIYTITNIWKTFGWNSILYLSSISSIDPNIYEAAKIDG
                     AGRLRQAINITIPSILPTIVILLVFAIGGLLSANTEFILLVYNPAIYERADVIGTYVY
                     REGLMSGNFSFGTAVGLFTSLINFGLLYLANASSRKLTDYALW"
     gene            complement(5936..8275)
                     /locus_tag="PK733_07075"
     CDS             complement(5936..8275)
                     /locus_tag="PK733_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10340.1"
                     /translation="MDVHVFKSSTFRKTLISYTVILMIPVFVFFYISLKSILTDNRQK
                     LIDIHTDNTQRLANTIDSKLIELMQIGDRLYYSKWINKLMMDIDTFDSEFNVLKKQEI
                     CQDLNNYVAFSGVLSNIIIVFPRKDIVVSQNAWYQVHEYFHNVLKIKQEDIENIYSYL
                     QDYTYFKTIDNFKVSTEDNLQSHTVVLRSLELNTNPRAVVLFFINDKYLNSYIEKIMP
                     PNLNELTITSSGTEIFSFNRGKMDDSINLNAADDKYPKKSGNSRRQNELSVFTAASSI
                     ALWEYKCIYDDTGLPVHIEQLLPVFLGIIFTLITGTLLALVLALISYKPLYSLFNKIF
                     KYNHTTKETYGKHLHEYTLIENSFNKLIDEKESILIRIKNYEDRMKSYESAARSNLLL
                     RLLKGYFEDIEDPKVIGGIRELGLNYTNDDYFCVTLMNINKVTEIGIEETVFKRQEII
                     DFLLIVEQIMNQVGIDCQILDALDNVIVVIMKFNQSDSNEILVHNLFSKIENISEIKY
                     SIKPIISIGKIEKGIIGISKSYQMSKENLGPSIFGAGTAGNSINILNNELYYYPTDWE
                     IQLINNLKIGNLDAVTRIIDEIRLENEKRNLTVHSLLRLLSLIMETELRVIAELNIDI
                     KFYQDTFNNIINLGDMKKSWSYIYEVSSRICDRSQYVNTESNLEVGNTILKYINDNFD
                     NPCLSLKELAQEFSMSASSLSKLFKEVTGINYYDYLCRLRIDKAKKLLEEPEYNISSI
                     ASKVGYENENSFRRTFKRYEGIAPNDYISKITSDNSSNN"
     regulatory      8655..8786
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9137..9709
                     /locus_tag="PK733_07080"
     CDS             9137..9709
                     /locus_tag="PK733_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ10341.1"
                     /translation="MKFDTNKMVKIAVLSALAVVLMLILRFPIIPSAAYLEYDPADIP
                     VLIGGFMYGPVAGLIITLIVSFIQAVTVSAASGWIGFAMHVIATGTLVIVSSLIYKKI
                     HNFKGAIIALIAGSIAMTLVMIPSNLYFGPKFGVPIEAVKAGLTVAVIPFNLLKSCIN
                     SVITMLLYKPLSRVLKGLGSERRLQAKTKN"
     gene            10028..12862
                     /locus_tag="PK733_07085"
     CDS             10028..12862
                     /locus_tag="PK733_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10342.1"
                     /translation="MIEKSAMLAKLPALIKYKVIKFAVILTVLIIAINLPVPVQVMAV
                     PEDAANNNYNNNNIEIRVEGGYSNTAKLGFYAPFFITVENKGQSISCEVQVIISTDSM
                     EKAAYAKQSDFPQNSKKLITIIAPIATANRKVEVRLVKGSKTIYSTKYDFANIIPPSN
                     PLIGILSEKQGALSNLSGLSMPENFISLTERDIDIKRKLMATAVETAVEMNADARTIQ
                     FTEQNFPDEEKALQAFDIIIISNYDTSQLNEKQLKLLDKWVNDGKMLFIGLGPNSMKT
                     YSGLGEEFKPFRIDGSSQIQKAAGLEKLIGKPLPNGGLNIVTGNTDNTGNVMYKNNGH
                     VILEEEGLPLAIAYNKGKGALVILTFDPTLKPVLDWEGEKDLWRKVIADAYAKTEMNT
                     GYMSAYGSGGYNAYSTRGINNLNFSYLASNVPETQRPPFTFLFVTIIIYVLIAGPVLY
                     ILLKRKDKRDLSWFTIPAIAVLFTLIIYIAGFKTRYTTAVLNNVSLINLENIQGTASI
                     YTTSAAFNNSRGSMAFSYNPLDSIEIGINSDYYGRPFYGAQPENDDNFKITSKLTYGA
                     ENSYQLYNAGMWEPKLIYINKTLPYSGKLISNAAVNKDGFRAIIENNTEFDFKESFIN
                     IGDYFFDIGDLRKGEKREIGFSFNDPSIKRNITDFLNDRYGDIYQPSSQRPPVDQWRE
                     MRRKREMMDNAYHNLVDTQHGGEKYGAFSKETKSIFFFALNYDDMGYKLAINGKQPIV
                     YNTNLIYSGSSMELKKGENIYLPAGIIVPVPDNMQNVYFDNRNKTMEVSENGNIDFSF
                     YLPENIDIKEMEITWQTSINKIFSFFIFNNKTSEWEEFDGMFRVTGSSGTGVNSSIGS
                     RDGSSTAVNSSSRDGSSTAVNSSSRVENSTSDSNSGDTDIYNIDAYTNNERQIKVRAI
                     ANFDKNVDKNFYRKVENIGVPEIELRGVVK"
     gene            12862..13908
                     /locus_tag="PK733_07090"
     CDS             12862..13908
                     /locus_tag="PK733_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10343.1"
                     /translation="MLVIENLVKQYGKFKAVDNLSMEIKEEQIYGFVGANGAGKTTTM
                     KIIAGLLKATSGKVALGGVDIEANPGRVKSRIGYMPDFFGVYDDLKVSEYMDFYAGVY
                     GIAKSKRKELSDQLIELVNLQDKTDVYVDTLSRGMKQRLCLARSLIHDPELLILDEPA
                     SGLDPGSRVEMKEILKELRKMGKTVLISSHILSELSEMCTSIGIIDRGKMVASGPVAD
                     IIKKASYNRVIRIKVTGSPEMAVKFLKEQPSVGSISEASDYIDADFNGNEQELASILR
                     GMIYANISVISFMELEKNLESAFMSIIRKNGGNSEKIGNGENCESLGNYENNENYEKQ
                     IKGGADYDYKKSGN"
     gene            13883..14809
                     /locus_tag="PK733_07095"
     CDS             13883..14809
                     /locus_tag="PK733_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10344.1"
                     /translation="MIIRNPVIEKELKTKMRGWKSPALITGYLAFLVLVIYLYFLASG
                     QLEKYSMQYFNPRVALNSYYFLASFQFGVLLLIVPALTGGAICGERERQTLDLLLCTN
                     FPAISIITGKLIVSIAHILLLLIASLPVLGSVFLFGGIGVSDILLLSLFYIVTAFMVG
                     SLGIFYSTIFKKTITSIIVTYITLLVLLFGTLIIMFVWGSVNRIQNFPRIEHLYAFLF
                     SNPIFGFVSIMEGLNGGYGISTIFGIGYGSTQLANTWVKPWMINAAFDILVTIVLVLI
                     STRRIKPVKGISKLKNKEGKKQFVFYSSRTKG"
     gene            14815..16569
                     /locus_tag="PK733_07100"
     CDS             14815..16569
                     /locus_tag="PK733_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10345.1"
                     /translation="MNIKYIDIYKAIAPLRRRMHVNRIIYYFIIALAVAGGISLILAS
                     IATFLPVTFLLRKVGLIYLILGVISLIISIIMKPDMLSICKKGDSLGLDERLVTAYYL
                     KDDNSPLACLQRENAFKKAGMTNFKSLYPIKLPKLEFAITVALAALVAATFFIPSAAK
                     EKAIAGENILKVVQKQAEEIAKEKEKIMEEALAFEEQLKDEQQKQLSEENHEKKIANA
                     TGKEQLEKINNEINRLLAELKKAGNEEEALKNLLLTENKLDEMLNGLLNKSIADGLKD
                     GIKDKAEDELKDLLEGELNDDLKDKLKGEFDSDSLDSDAVNNLRANNADSVDIADIEK
                     ALESLIAAINNAKNEIADISGNSSLNLAQGSSTGNSSGNTSGENAGQSAGENAGQGTG
                     ESVGQSYREGNGEGSGQGVEQANNGDTGQGSGQGTEQGAGQANGEGIGQGSGQGTTQS
                     TGQGAGAGQGAGQGAGQGAGEGTDSGSNSYSEENSGRNASGKNPGERQVKDYERIYVP
                     ERLGGNTDSSLVKGNMNDSGDSVFYEAKGSPVEKGYMMPYNEVLNQYKSQAIMGLENH
                     PVPPGMKEIVKDYFSSLD"
     gene            17063..19402
                     /locus_tag="PK733_07105"
     CDS             17063..19402
                     /locus_tag="PK733_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOJ10346.1"
                     /translation="MRDNLWEFVKEEFDSSLPRMTAIYVKEEDESLFFSYPVEGDRLG
                     YYFNFTDKTGYDIPPKFRIDFAVYEYPSIMRLLANNRVLFKKQEYYPSMIKRNFTYTN
                     LGLVGTERMGQRKNGSLTWLFDFKCINEGFKFEKQLFSILSSDEEFTVAKENEGLIIK
                     FEDINYYIACSNIGKYGVYPEELSMKKDLSSGAITSNSVSGHYLVIAHDMNIFPGEKA
                     ALAFGLSSISSSKAVEAVQIEKTNLVEACEAAQLKEASFGEDFENDISKRWNNWFTSL
                     PYIKLSDEREKKAYYKCWWVIKNNYYNHPEWGFNITESLPVYKGLWQWAIPSIQWHSD
                     QNTEYTSQWIKKGMDMFVNSQREDGYITHAIYIDEEKPGERWAKGGGIIQTPHLPWVA
                     LRHYNSTGDLEALVRWYPAFVKYYDYICRTRDQKFKNIHLWGIVTSFDTGLDTTSAFQ
                     RVTYGENGVKEAYCYPSIFAAERCRYEMAMGEIAEILEQNIKNITDIKYNENITGIKN
                     NVYSKWSKKEWYREAEKTREAMNSHLWDSDKKWYGVLHEDGTLDTRVGVDGMFPLVYH
                     LVEPERAATMEENFMKLIGNYGIRTVAPGEPGFRADVYWRGAAWPKPCSLGMEICRYY
                     FPHLMDRAHQAILAMALCYPSIWECVNAETGELARSDHGFICTPGMSSNVGAGDIIGS
                     IWMYHGLPMYDMDMVLPLVEMKDFHWRGMRITAEKENGCWKICGNSVEKDRAVVHFYK
                     IAETAKIGDAAEPKEENDINSGKVCEVKLCNGESVMVNL"
     gene            19784..>20509
                     /locus_tag="PK733_07110"
     CDS             19784..>20509
                     /locus_tag="PK733_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10347.1"
                     /translation="MIDMVLKIEKEISKHMIGQKELIRQVVIAILCGGNVLLEGVPGL
                     GKTRLVKTIGRVLDLKFSRIQFTPDLMPADVMGTNIIIKSSEGEGGFRFQKGPVFSNI
                     VLADEINRATPKTQSALLEAMQEQTVTAGGTTYSLPQPFFVLATQNPIEMEGTYPLPE
                     AQMDRFLFKLIVPFPSLQELSEIVTMTTGGPEKELRAVCSGNDIMSMRNIAAEIPIAK
                     PVLEFAMKLVLATHPESPYATEKT"
BASE COUNT         6886 a         3232 c         4126 g         6265 t
ORIGIN      
        1 ctttttaaag aaaaagagga ctttgaaaga ttgatggctt tgtattatga tggagctgcg
       61 cagagagtag gcaagttgct ggatgaaaga taggatggca gggtggcagg aacatgattt
      121 tgtgaatttg ctgataaaat gataaaatat aaatggttat tatgataaaa tataaatgat
      181 aatgaacacg tagttaagta tacttcatta ttattttaag gattgtgata cttatgattc
      241 cttattacga agagattgaa aatataaaaa atcagatatt aagcaaatat aaccctaaag
      301 atattatact gtttggctct tgcgcaaaag ggagagtatc ccgtacgagt gatatagata
      361 tctgcgtaat tttagatact actgataagc ggcaaattgt cagggaaatg ctagttgata
      421 ttgaatcaga tgccgatttg gatatagtag tttacacacc tgatgaatgg gagaaatata
      481 aatatgataa ggctaaattt gcaaatataa ttaataggac aggagtgagt attattggtt
      541 gatagcacca agtgtaatga atggtttgtt atggctcaaa aggatattaa aggagccaaa
      601 attttgtttg aaaatgatgc agataatgag attgtgtgtt ttcattgcca gcaggctgtt
      661 gacaaatatt taaaaggata ccttatatat attactggtg aattacaaga agggcatagt
      721 cttgtaaaac tttgcaaaaa aataatgaag tatgacaaaa catttggaga gtttttaaag
      781 gacatggctt ttattaatac cttttatatt gagactagat atccagcagc tgacccgctt
      841 attgtaacaa aagaagatac tgaagagtgt tttaaaataa ctcaaaatgt gatctcaaag
      901 ataaatagct taataagttg aataccaagc aatgcaaata tagatagtac tgctgattat
      961 aataaaaacg atatagaaag caataagagt accaggagat ataatatggg gaataaaaat
     1021 gtagataaag gcaaaatata taaaacattt gaaaaggatc tttatgctcc tgtacgaagc
     1081 tacttaatgc agcagggctg ggatgtaaaa ggtgaagtaa aatattgtga tgtagcggct
     1141 gttaaagatg acgtttttct tgtggtagag atgaaaacca gtcttaatct tgatgttata
     1201 ctgcaggctg ccattagaca gagaacagct gatatcgtgt atattgcagt acccaaaaac
     1261 agcagaatcc ttttcacaaa acgatggaaa aatatcttat accttcttag aagacttgaa
     1321 gtaggtcttt tgcttgttga tttaaaattt caatcttttg cggatgagaa agtacagcat
     1381 aatgaggcgg gagttagcaa ggcaagggtt gaggaagcgt taaagccgga gccttttaac
     1441 aggaatatga gccgcaaatc atctggcaaa aagagggatt tgatattaaa agagtttggc
     1501 gcccgctatg gtgattataa taccggagga gttacaggcg aaaagctggt tacggcgtat
     1561 agagagctgg cactacatat tgcagcacta ctggataaga atgaaaaact tagcataaaa
     1621 gagcttaagg ccttaggcac ggataataaa aagactgcaa aattgcttca ggataatttc
     1681 tatggttggt ttcagcgagt ttccagaggc gtctatgaat tgagcgaaaa tggtaaagag
     1741 gaaatagagg agtacaggga gattatggaa cactatttga agaaatgtta aaatgcatca
     1801 tctgaatata ttgagggacg cttctcaatt aacccattct tttacagagt tttctttatt
     1861 taaactccta aatattaatg gttttcaaga agcgttccca tatttagatt tatgagccct
     1921 tttgctattt aagctcaaaa tatctgtcaa ggagctgctg atccatttca atcagttgat
     1981 ccagacctaa cttctttagc tcagttatat aaacatccca atcatcaagg gacttttgcc
     2041 ctagagcaag tttcgcagct aattcagctg aataggtttt caaatcattg agtacctttg
     2101 tctttctttc aagttgttct gtattaggaa ttgcaaggta attctgattt tgatttacat
     2161 agtaaggaga ataatcgatt attttcagtt gatgatcttt tttatgttga ggtatatttg
     2221 ccaattcata ttcaagattg gcaaattgga ctcttgggaa tacagtatct ccaaaaacct
     2281 gattaccggc tattttcccg ctatccgcca atttggtatc gaaagaaccg gttatgtcaa
     2341 caaattctct gatcccatcg tcatttacct tatatgtgaa atctttaatg ccccagaaaa
     2401 ggagatctgc atattcctta gaataaataa catcaaaaaa tgatattgca gcatccgggt
     2461 ttttgcagtt tttggtaatt gcgtattttt tccacacgag gaaaggtggc tccttttgtg
     2521 cagcaggttt gattccttca acagctttaa gcggcataag aggggtatat tcacctccgc
     2581 cctgtatagt gggctcccta tatgtttcca tgttatagtc ggataatgat gctgccttgt
     2641 tttctgttat tttctgctga actgcttcag aacctgcacc aataaggctg gtatccaata
     2701 tttcttcttt aaccagagtt tggacatatt taaaatattc ttttatacct tcctgatacc
     2761 acggtgaaac tacttttttg ttttctatat caactgctgt aatatctgtt ccaagaccaa
     2821 accattgagc aatagcacct ttgaaaactg aaggatcata tataagtatt tcgtcttcct
     2881 tgccgctggc atttgcatcc tgctttctca tagtcttcaa tgcatttaag tattcctgag
     2941 cagtagttgg agcaggaata ttcaatttgt ccagccaatc tttcctgatt atcatagtta
     3001 gagcaacagg tgcaggttgt gttcctttat acaacttaat atggagatta ctgaaccaat
     3061 acattttgcc atcaggcgat gttgtcaatt gttttgcaaa tgggaaatcc ttagtataca
     3121 tattctttat gtttccatta ctgtatttat caataagtga atttagctca agaatggtat
     3181 tctgttttgc cagatttaaa actgtagtat catccaatgg cgagagattg acaatatccg
     3241 gaagatcacc tgctgcagcc attcttgttt gtattactac attatactgt tcaaaaggca
     3301 caatttcata ttttatctca aggttttcct gatcaagcag tttctgaact tccttccata
     3361 caggaaactt ttctctatcc ccaaacttaa caaccttacc gcttttatcg ggccccagca
     3421 catgaagttt tcttggctgt tttgcttcac ttcctttttc atccttattc ccagtgtctg
     3481 ctactttatt gctattggaa gtttgtcctc ctgaatcttt tataacacat ccgaccgaag
     3541 ttaacagcaa gcttatgatg actaaaacaa ttgctaatga cttaaacttt ttcattatta
     3601 aatacctccc tgtgatatag tttaatatat ttatgtgctg cactatttat tattgcagca
     3661 gcattaaacc aagttaaata tgaaaaggaa agaagtccta atatcagaca ctatcaatat
     3721 acatatgtgc acttaccctt ttaaggatcc cagcataacc ccctttataa aatacttttg
     3781 gaaaaatgga tatgtaaaaa ttataggcag agttgtaaaa actataattg agtactttag
     3841 ctgcatattt gtaaaaatca tttctgcagc ttccgccatt tcgtcaaagg ctagttcctt
     3901 taaatctaca gtttgctgga ctaggacacg gtaaagatac atttgcaatg gatgaagcat
     3961 ttcatttggc aaataaacca gagcactgaa ccaactattc catataccta ctatagtata
     4021 tattgaaatt actgcaagaa tcggcttgga tacaggaaca actattttaa ataaaagctg
     4081 aaaatgattg gcaccgtcta taaatgcaga ttctttcata gaatccggta tagttatgaa
     4141 gtaagtcctg acaagaataa tgtaccatat acttactgca cctggaatta taattgccca
     4201 tctggtatta tacaacccaa gtttatttat aagtaaaaag gtcggaatca aaccacctcc
     4261 aaaatacatt ggaataataa ggtaacttac tagccattta cgccctgcaa ggttgcgtac
     4321 acttaaaggg tatgcaccaa gtatggatgt taatagggtt aaaaaagtgc cgcatataac
     4381 atagattagt gtgttcatat atgcccacca catttttgaa tcacgaacta ttaacctgta
     4441 tgacccaagt gtaagaccct ttggatatag atatacatcc attgatgctg cttttattgg
     4501 tgaacttata gacataataa ctacataata aaaagggtaa agtgtcacta tacatattaa
     4561 tacagcaaag aaatagagca tgaagtttgc aaatgaagtg ccttctttaa ttctgccgga
     4621 atttttgctg caagggtttt tataggactt tatattgaag tttatactgt tatcaaccat
     4681 gttgttcata gccctccttt tattctctat cctaatatcc ctaaacaatg ctcatactaa
     4741 tatagctacc agctaccgca gtatgttgtt accaaagcat attaccaaat tctatcataa
     4801 aagtctatta ccaaagtgca tagtctgtca gctttctgct ggaggcattt gccaagtaaa
     4861 gcaacccaaa atttatcaac gatgtaaata gcccgacagc agtaccaaaa ctgaaatttc
     4921 cactcataag tccttcccgg taaacataag taccaataac atctgctctt tcatatatag
     4981 cagggttata tacaagaagt ataaattccg tgttggctga aagcagacct ccaatagcaa
     5041 atacgagtag tattacaatt gtaggaagaa tggatggtat agtaatgtta attgcctgcc
     5101 tgagcctgcc ggccccatca attttagctg cttcatatat atttgggtct attgatgata
     5161 tagaagataa atataaaata ctattccaac caaatgtttt ccatatatta gttatagtat
     5221 atattgcagg aaagtatcca ggttctgtat tgaagggaat tgccttttta ccaagcgact
     5281 taattatgga atttatgaca ccatcagtat taataaaaga taataccatc cctgcaacaa
     5341 caacagttga aataaagtat ggaagataac ttgcagtttg tacaaacttt tttaaaaaac
     5401 cattaccgag ttcattaata agtaaagcaa atactatagg cacccagaat ccgaaaatta
     5461 atatatataa gctaagtgaa aatgtgtttg taaatagtct tccaaagtaa ggtcctgaaa
     5521 aaaatttcac aaaatgtttc agaccaaccc aactgacact tccatctagt gcaataaatg
     5581 gactgcctgg ataataatcc tgaaaagcaa ttgccacccc atacataggt atataatgaa
     5641 aaataaatat gtggaggaga actggcagca ttataaggta tagctggata ttctcttgta
     5701 tatgacgatg ttttgatggc gtgacaatta ttttgttttt cgtataatat agtatgctgt
     5761 ttaccataat tattaaatct cctcattaac aattactaat cactgaataa atattatttt
     5821 ttgttgccgc actgttataa tagcacttgc ccttttcttg agcaagaacc aatattttat
     5881 atgctcagca attttttctt aacagctttt aggttaagaa aatcggcata cactattagt
     5941 tattactgct attatcactg gtaattttag aaatgtagtc atttggagcg attccttcat
     6001 atcttttgaa tgttcgtcta aatgaatttt cattttcata tccgacttta cttgcaatgc
     6061 tgctgatgtt atactctggt tcttcaagca gtttttttgc tttatctatt ctaagcctgc
     6121 ataagtaatc ataataatta atacctgtta cctctttaaa aagctttgac agtgaggatg
     6181 ctgacatgct aaattcttga gcaagttctt taagagaaag acaggggtta tcaaaattgt
     6241 cattaatgta ttttaatata gtattaccaa cttcaagatt ggattcagta ttaacatact
     6301 gacttctatc acatatgcgg ctgctgacct cgtaaatata tgaccatgac tttttcatat
     6361 ctcccaggtt tataatatta ttaaaagtat cttgataaaa ctttatatct atattaagtt
     6421 ccgctattac tcttagttct gtctccataa ttagagaaag caaccttaat aaagaatgaa
     6481 ctgtaagatt tctcttttca ttttcaagcc tgatttcatc aatgatcctt gttacagcgt
     6541 ccaggttgcc gattttaaga ttgttaataa gctgtatctc ccaatcagta ggataataat
     6601 acaattcatt attcaatata tttattgagt tacctgctgt accggctcca aatatagagg
     6661 ggcccaggtt ttcttttgac atctggtagg atttgctgat accgattatc cccttttcaa
     6721 ttttaccgat tgaaataatt ggtttaatac tgtattttat ttctgatata ttttctattt
     6781 tcgaaaataa gttatgtaca agtatctcat tgctatctga ttgattaaat ttcataatta
     6841 caactataac attgtccagt gcatcaagta tttggcagtc aatgccgacc tgattcatta
     6901 tctgttcaac tataagtaaa aaatcaatta tttcctgcct tttaaaaaca gtttcttcta
     6961 taccgatttc tgtgacttta tttatattca taagtgttac acagaaataa tcatcatttg
     7021 tgtagttgag gcccagctct ctaattcctc caataacttt tggatcttca atatcttcaa
     7081 aataaccctt aagcagacgc aataacaaat tagaccttgc agcactttcg tagcttttca
     7141 tcctgtcttc gtaattttta atcctgatta atatagactc tttttcatct atcaatttat
     7201 tgaaggaatt ttctattagt gtgtattcat gcaaatgctt tccatatgtt tcttttgtgg
     7261 tatgattgta tttgaatatt ttattaaaca atgaatataa tggtttataa ctaattaatg
     7321 caagtacaag agcaagcaga gttccggtta tcaaagtaaa tattatacct aaaaacacag
     7381 gcagcagctg ctctatgtgt acaggtaatc cagtatcatc atatatacat ttgtattccc
     7441 atagagcaat acttgatgct gcagtaaaaa cagataattc attttgtctc ctggagtttc
     7501 cggatttctt tggatattta tcatcagccg cattaaggtt tatactgtca tccattttac
     7561 cccgattgaa tgaaaatatt tcggtaccgc tgcttgtaat agttaattca tttaaattag
     7621 ggggcattat tttctcaatg tatgaattta agtatttgtc attaataaaa aataatacca
     7681 ctgctcgagg gttggtattt aattcaaggc ttcttaaaac aacagtatgg ctttgcaaat
     7741 tatcttcagt actaaccttg aaattgtcaa tagttttaaa ataggtatag tcttgcagat
     7801 atgaatatat attttcgata tcctcctgtt ttattttgag aacattatga aaatactcat
     7861 gaacttggta ccaggcattt tgggatacaa caatatcttt tctggggaat actattataa
     7921 tatttgatag tactccggaa aaggcaacat agttattaag atcctgacag atttcctgct
     7981 tttttagaac attgaattca ctgtcaaaag tatcgatatc catcataagt ttatttatcc
     8041 attttgaata gtaaagtctg tcccctattt gcatcagttc tattaattta ctgtcaatag
     8101 tattagcaag acgctgcgta ttgtcagtat gtatatcaat aagtttttgt ctgttatccg
     8161 ttaatatgct tttgagtgat atgtaaaaaa acacaaatac tggtatcata agaataacag
     8221 tatacgagat aagtgttttt ctaaaagtac tgcttttaaa aacatgaaca tccatatgca
     8281 ttctcctgtc tccaaaagta aaaaacctgc taaattaaaa aatacagttt agaactaaca
     8341 atttaagtat aaatataatt ataagttaaa tcaaggtatt atttataccc gggtgcattt
     8401 tcttcctatg atacaaaata tggcagcata cgatttcccg agtataaaat ttgaacaaag
     8461 cttacggatt gtagacttaa acactacaat ccgttgctga agtgaatatt atacatggaa
     8521 atcgtttctt gccatatttt tcttcaaata tagaaaatgc acccatttat acccggatgc
     8581 atttaattta tctgaagaaa atattgaccc ttcataagaa ctgtgctatt attattttat
     8641 aaattaaata tgaaagtaat cttcagggca gggtgtaatc cccgaccggc ggtaatacgg
     8701 acatataaat ccgttagccc gcgagccaaa gctgatctgg tgagattcca gagccgacag
     8761 taaagtctgg atgaaagaag attttttgca taacgtggca taaaaggttt ttatcaatgg
     8821 aaatgatgct taagagtcaa tcattaaacg cctcataata caatgccagg tggtgtagga
     8881 attaaattcg agtatatgag ttccctgaag gttattcagg gaattttttg ttgccgcgaa
     8941 acaagaactt tcctgaaatg aatggattta taaagggaaa cctgaggtcc actaacgaac
     9001 actaatgaat aagaagattt gcgcaactag ttttattaat agctaaacgc attttcttaa
     9061 tgatatttga ttgtagtgaa tttaggtacc gaaatgctag gtagctttta aaaaatttgt
     9121 gaggagagtg ttttttatga aatttgatac taacaaaatg gttaaaattg ctgtattatc
     9181 tgctttggct gttgtgctta tgcttatact gagatttccc ataataccgt cagcagcata
     9241 tctggagtat gatcctgcag atattccagt actcattgga ggatttatgt atggacctgt
     9301 tgcaggacta ataataaccc tcattgtttc attcattcaa gcagttacag taagtgcggc
     9361 aagcggctgg ataggttttg cgatgcatgt cattgcaact ggtacattgg ttatagtttc
     9421 atcgcttatc tataaaaaaa tacacaattt caagggtgct attattgcac ttattgccgg
     9481 gtccatagca atgactctgg taatgatacc ttcaaatcta tattttggac ctaaatttgg
     9541 tgtacctatt gaagcggtta aagccggatt aacagtagcg gtaatacctt tcaatttgct
     9601 taaatcctgc ataaactctg ttataacaat gttgctctat aaacctttaa gcagggtact
     9661 gaagggtttg gggagtgaaa gaaggttgca ggcaaaaacc aaaaattaag actttaaatt
     9721 cgagctaaag ggactcactt ttatttcaag aaatatccct aataaattgt gctgagaagt
     9781 atttccaata attcaaaaaa acaaaaataa tgatttttgg ctaaaacacg agacgtaaat
     9841 cctggagtat atctgcccca ggattttttc aattacagaa acttgaaaac ctgatgttaa
     9901 tatgatatat tcaaaaaatg taatagaact tttatcatat ttgaagagtc taaatattaa
     9961 tagatatttt agccttgtaa aggcattttt gtgttatata tagtattttg aaaggggaag
    10021 atttttattg attgaaaaat cggcaatgct tgccaagctt ccggcactaa ttaaatataa
    10081 agtaataaaa tttgctgtaa tattaactgt tcttattatt gctataaatt tgccagtacc
    10141 tgtacaggtt atggctgttc ctgaagatgc tgcgaataat aattacaata ataacaacat
    10201 agaaataagg gttgagggtg gatacagcaa cacagcaaaa ttgggatttt atgcaccatt
    10261 ttttataact gttgaaaata aaggacaaag tatcagttgt gaagtacagg taataataag
    10321 cacagattcc atggaaaaag ctgcttatgc aaagcagtcc gattttcccc aaaactcaaa
    10381 aaaattaatt acaattattg ctcctatcgc tactgcaaac agaaaagtag aggtaaggct
    10441 tgttaaaggg agtaaaacta tttatagcac gaaatatgac tttgcgaata ttataccccc
    10501 ttcaaatcct ttgataggta tattatcaga aaagcaggga gctttatcca atttgtcagg
    10561 cttaagcatg cccgaaaact tcatatcact aaccgagagg gatattgata ttaaaagaaa
    10621 acttatggca actgctgttg aaacagcagt agaaatgaat gcagatgcaa ggaccattca
    10681 atttacagaa cagaactttc ctgatgaaga aaaggcactt caggcttttg atattattat
    10741 tataagcaat tatgatactt cacaattaaa tgaaaaacag ctaaagcttc ttgataaatg
    10801 ggtaaatgat gggaaaatgc tttttatagg tcttggacca aattcgatga aaacatattc
    10861 aggattggga gaagaattca aaccgtttag gatagatggc tcaagtcaaa ttcaaaaagc
    10921 agctggtctt gaaaagctga tagggaaacc attacctaat ggaggtttaa acatagtaac
    10981 cggtaatacc gataatactg gcaatgttat gtataaaaat aatgggcatg taatattgga
    11041 agaagaaggc cttcccttgg ctattgctta caataaggga aaaggagcct tggttatcct
    11101 gacttttgac cctactttaa agcctgttct tgattgggaa ggtgaaaagg atttatggag
    11161 aaaggtaatt gctgatgcat atgcaaaaac tgaaatgaat acagggtata tgtctgcata
    11221 tgggtctggc ggatataatg cttactctac taggggcata aataatctga attttagtta
    11281 tcttgcgtca aatgtgcctg aaacacagag accgcctttt acatttctgt ttgtaaccat
    11341 aatcatatat gtactgatag cagggcctgt cttatatatt ttgctcaaac gtaaagataa
    11401 aagagatcta agctggttta ccataccggc aattgctgtg ctttttacct taataatata
    11461 tatagcgggt ttcaaaacaa gatacacaac agcagtattg aataatgttt cactgataaa
    11521 tctggaaaac attcagggta ctgccagtat atatacaaca tcagctgctt ttaacaacag
    11581 cagaggcagt atggcattca gctataatcc tttagactct atagagatag gaattaacag
    11641 tgactattat ggaaggcctt tctacggagc tcagccggaa aatgacgata attttaaaat
    11701 aacatccaaa ctgacatatg gagcagaaaa ttcttatcag ttatacaatg caggaatgtg
    11761 ggagcctaag cttatatata taaataaaac acttccctat tcaggaaaat taataagcaa
    11821 tgcagctgta aataaggatg gctttagggc gattatagaa aacaatacag aatttgactt
    11881 taaagaatct tttattaata ttggagatta tttttttgat ataggagatc tgcgtaaagg
    11941 tgaaaaaagg gaaataggat tttcctttaa tgacccttct ataaaaagga atattacaga
    12001 cttcctaaat gaccgttatg gtgatattta tcagccttca tcacagaggc ctcccgttga
    12061 tcaatggagg gaaatgagac gtaagaggga aatgatggat aatgcatatc ataatctggt
    12121 tgatacacaa catggtggag aaaagtatgg agccttttct aaagaaacta aaagtatatt
    12181 cttttttgct ttgaattatg atgacatggg atataaatta gcaataaatg ggaaacaacc
    12241 aatagtatat aatacaaatc taatttattc tggaagcagt atggaactta aaaaaggaga
    12301 gaatatttat ctgcctgcag gcattattgt gcctgttcca gataatatgc aaaatgtata
    12361 tttcgataac cgcaataaaa ctatggaagt atctgagaat ggaaatattg attttagttt
    12421 ttatttgcct gagaacattg atattaaaga aatggaaata acctggcaaa catcaattaa
    12481 taaaatattt agttttttca tatttaacaa taagacttcg gaatgggaag agtttgacgg
    12541 gatgtttagg gttacaggca gcagcggcac cggtgttaac agcagcatcg gcagtagaga
    12601 tggaagcagc actgctgtta acagcagcag tagagatgga agcagtactg ccgttaacag
    12661 cagcagtaga gttgaaaaca gcacttcaga cagtaattca ggtgatactg atatatataa
    12721 tatagatgca tatacaaaca atgaaagaca aataaaggta agagctattg caaattttga
    12781 caaaaatgta gataagaatt tttatagaaa ggttgaaaat attggtgtac ctgaaattga
    12841 attgagaggg gttgttaaat agtgcttgtt atagagaatc tggtaaaaca atacggaaag
    12901 tttaaagcag tagacaatct ctccatggag ataaaagaag aacaaatata tggatttgtg
    12961 ggagctaatg gtgctggaaa aaccacaaca atgaagataa ttgcaggact gctaaaagca
    13021 acttcaggga aagtggcttt aggaggggtg gacatagaag ccaatcccgg gcgggtaaaa
    13081 agcaggatag gttatatgcc tgattttttt ggggtatatg atgaccttaa agttagtgaa
    13141 tatatggatt tttatgccgg ggtttacggc atagctaaaa gcaaaagaaa agaattgtca
    13201 gaccagctaa ttgaacttgt taaccttcag gacaagacgg atgtttatgt ggatacctta
    13261 tccagaggca tgaagcagag gttgtgcctc gcaagaagtc ttattcacga cccggagctc
    13321 ctaatcctgg atgagccggc ctcaggtctg gatcctggat caagagtgga aatgaaagaa
    13381 atccttaaag aactgcgtaa aatgggaaaa acagtactta tcagttcaca catattatcg
    13441 gagctttctg aaatgtgcac ctccataggg ataattgata gaggaaaaat ggtggccagt
    13501 ggtcctgttg cagatataat taaaaaggca tcctataaca gggttattag aatcaaagta
    13561 acaggaagtc cggagatggc agtcaagttc cttaaagaac agccttctgt tgggtccata
    13621 tctgaagcca gcgactatat tgatgcagat tttaatggta atgagcagga attggctagt
    13681 atattaagag gaatgatata tgcgaacatt tcagtaatat cttttatgga attggaaaag
    13741 aatcttgaat cggcttttat gagtataatt agaaaaaacg gtggaaatag cgaaaaaatt
    13801 ggaaatggtg aaaattgtga aagccttggg aattatgaaa acaatgaaaa ttatgaaaag
    13861 caaataaagg gtggtgcaga ttatgattat aagaaatccg gtaattgaaa aggaactgaa
    13921 gaccaagatg agaggatgga aatcccctgc actcataaca ggttatcttg cttttttggt
    13981 tctagtcata tatctttatt ttcttgcatc cggacaatta gagaaatata gtatgcaata
    14041 ttttaacccg agagtagctc taaactcata ttattttctt gcatcattcc agtttggtgt
    14101 acttctcctc atagttcctg cattaaccgg aggtgcaata tgcggagaaa gggagaggca
    14161 gaccttggat ttgctattgt gtacaaattt ccctgctata tccattataa ccggaaagct
    14221 gattgtttca atagcccata ttctgctatt gctcatagct tctctgcctg tcctgggttc
    14281 ggtatttctc tttggaggaa ttggagtttc ggatattcta ctgctatcat tgttctatat
    14341 tgttactgca tttatggtag ggagtctggg cattttttac tcgacgatat ttaaaaaaac
    14401 cataacatca ataatagtta cttatataac tttgcttgtt ctgctttttg gaactttaat
    14461 aataatgttt gtctggggct ctgtaaatag gattcaaaat ttccctcgaa tagagcatct
    14521 atatgcattc ctgttctcaa accctatatt tggctttgtg tctataatgg aaggactaaa
    14581 tggagggtat ggtataagta caatctttgg aataggctat ggcagcacac aactggcaaa
    14641 tacatgggta aagccttgga tgataaatgc agcttttgat attttagtta caattgttct
    14701 tgtactgata agcacaaggc ggattaagcc tgtaaaagga ataagcaaac taaaaaataa
    14761 agaaggaaag aagcagtttg ttttttattc tagccggacg aaaggttgat tttgttgaat
    14821 attaaatata ttgacatcta taaagcgatt gcgcctttga gaagaaggat gcatgttaat
    14881 agaataatat attattttat aatagcattg gctgttgccg gaggtatctc tttaatactg
    14941 gcatcaattg ctacattttt gcctgtaact ttcctcctga ggaaagtagg gcttatatac
    15001 ctgattttag gtgtaatttc cttaattata tcaattatta tgaagcctga catgcttagc
    15061 atctgcaaaa aaggtgattc tctgggactt gatgaaagac ttgtaacagc atattactta
    15121 aaggatgata attctccctt ggcctgcctg caaagagaaa atgcctttaa aaaagctgga
    15181 atgactaatt ttaaaagcct ttatccaata aaacttccaa agcttgagtt tgctattact
    15241 gtagcattgg cagcccttgt tgctgcaacc tttttcatac catcagcagc aaaagagaaa
    15301 gccatagctg gggaaaatat tttaaaagtg gttcaaaaac aagcagagga gatagctaaa
    15361 gagaaggaaa agataatgga agaggcactg gcatttgaag agcagttaaa agatgaacag
    15421 cagaaacagc ttagtgagga aaatcatgag aagaaaattg ccaatgcaac tggcaaagaa
    15481 caactggaaa agatcaataa tgaaattaac aggcttcttg cggagttgaa gaaggcgggg
    15541 aatgaagaag aggctctaaa gaacctgcta ttaacagaga ataagcttga tgagatgctt
    15601 aatggattat taaataaatc aatagctgat ggattaaagg atggaataaa ggataaagca
    15661 gaggatgaac tgaaagattt actggaaggt gagctaaacg atgatctgaa agataaacta
    15721 aaaggtgaat ttgattcaga tagtttggat tcggatgcag ttaataatct aagggcgaat
    15781 aatgcggata gtgtggatat tgcggatatt gaaaaggctt tggaaagctt aattgcagcc
    15841 ataaataatg cgaagaatga aatcgcggat atatcaggga atagcagcct aaatcttgcc
    15901 cagggaagta gtacaggcaa ttcttccggt aatacttcag gagaaaacgc aggtcagagt
    15961 gcaggcgaaa atgcagggca aggtacaggt gaaagtgtag gtcaaagtta tcgcgagggc
    16021 aatggtgaag gcagcggtca aggcgtggag caggctaata atggagacac aggccaaggc
    16081 tccggccagg gtactgaaca gggcgcaggg caagctaatg gcgaaggtat aggtcagggc
    16141 tccggccagg gaactacgca gagtactggg caaggcgctg gtgctggtca aggtgctggt
    16201 cagggtgctg gccagggtgc tggagaagga actgatagtg gaagtaatag ctatagtgag
    16261 gaaaactctg gaagaaatgc aagcggaaaa aatcctggag aaagacaggt aaaagattat
    16321 gagaggatat atgtacctga aagactagga ggaaacactg attcatcttt ggttaaagga
    16381 aatatgaatg attctggtga cagtgttttc tatgaagcaa aaggctcgcc tgtggaaaaa
    16441 ggatatatga tgccttataa tgaggtttta aaccaatata aaagtcaggc aattatgggc
    16501 cttgaaaatc atcctgtacc tccgggtatg aaggaaattg tgaaggatta tttttcttcg
    16561 ttggattgag taatgtatat ctagttccca gatatagtgt ttcctaagtg caggatgaaa
    16621 tgatgcaaat tccaatatga tgctgatgaa tgaactttta tgctggaatt gcgacgacat
    16681 tataccatga atttgctaaa attcatggta taattgtgca tgtgcgtttt ttgcaaagca
    16741 aaaatattcg cgcacacata aattccgccg gaggcatata atatcctcac ttgcggataa
    16801 tacgaggata ttatatcatg agctttgaag aaattgcatg atataattgt gcatatgcgt
    16861 tttttgcgaa gcaaaaatat tcgcacgcac ataaattccg ccggaggcat ataatatccg
    16921 aattcatgag gatattatat caggttatat caagttgttt agccatatgg catcatgtgt
    16981 gtgatataat aagcataata ttaacagcat aatagcttat aatagcaaaa aactttatat
    17041 gtaaaaggag caacaaatct gtatgagaga taatttatgg gaatttgtta aagaagaatt
    17101 tgatagcagt cttccaagaa tgacagctat ttatgtaaaa gaagaggatg aatccttatt
    17161 tttttcttat cctgtagaag gtgataggtt agggtattac tttaatttta ctgataaaac
    17221 cgggtatgat attccgccaa aattcagaat agattttgcg gtgtacgaat atcccagcat
    17281 aatgaggctg cttgcaaaca accgggtact gtttaaaaaa caggaatact atccctctat
    17341 gataaaaaga aactttacat atacaaacct tggattagta gggaccgaga gaatgggaca
    17401 gagaaaaaac gggtctctta cctggctgtt tgactttaaa tgtattaatg aaggatttaa
    17461 atttgaaaag caactttttt caattctttc ttcggatgaa gaatttactg ttgctaagga
    17521 aaatgaaggc ctgataataa agtttgaaga tattaattac tatattgcat gttcaaatat
    17581 tggtaaatac ggtgtatatc cggaagaact atctatgaag aaggatttaa gcagcggagc
    17641 tattacaagt aattcagttt caggtcatta tcttgttata gctcatgata tgaatatttt
    17701 tcctggagaa aaagcagctt tggctttcgg gcttagcagt atttcttctt ctaaggctgt
    17761 tgaagcggtg caaatagaaa agactaatct ggtggaagca tgtgaagcag cacaattaaa
    17821 agaggcaagt tttggtgaag actttgagaa tgatataagt aaaagatgga ataattggtt
    17881 tacatcgctg ccatatataa agctctctga tgaacgggag aagaaagcct actacaagtg
    17941 ctggtgggtt attaagaaca actattacaa ccatccggaa tgggggttta acattactga
    18001 atcacttccg gtatacaaag gcttatggca gtgggcgata ccttcaatac aatggcactc
    18061 tgatcagaat acagaatata cttctcaatg gattaaaaaa ggtatggata tgtttgtaaa
    18121 cagccagagg gaagatggat atataaccca tgctatttat attgacgaag aaaagccggg
    18181 tgaaagatgg gcaaaaggcg ggggaataat tcagacgcct catctgccct gggttgctct
    18241 aagacattat aactcaacag gcgatctgga agcccttgtc cgctggtatc ctgcttttgt
    18301 aaaatactac gattatatat gcaggacacg tgatcagaaa ttcaaaaata ttcacctgtg
    18361 ggggattgta acttcctttg atacaggcct tgacacaacc agtgcattcc agagggtaac
    18421 atatggagaa aacggtgtaa aagaagccta ttgttatcct tcaatatttg ctgctgaacg
    18481 atgccgctat gaaatggcaa tgggggaaat tgcagagata ttggaacaga atataaagaa
    18541 tataacagat atcaaatata atgagaatat aacaggcatt aaaaataacg tgtatagcaa
    18601 atggagtaag aaagaatggt atagagaggc agaaaaaacg agagaagcta tgaattctca
    18661 tctatgggat tcagataaaa agtggtacgg agttctccat gaagatggca cccttgatac
    18721 aagagtgggc gtagatggca tgttcccatt ggtttaccat ctggtagagc ctgaaagggc
    18781 tgcaacaatg gaagaaaact ttatgaaact tattgggaac tatggtatac gcactgttgc
    18841 acccggtgaa cccggtttca gggcggatgt ttattggaga ggtgctgcat ggcccaagcc
    18901 atgttctctt ggaatggaaa tttgccggta ttatttcccg caccttatgg atagagctca
    18961 tcaggcaata ttggcaatgg ctttgtgtta tccaagtatt tgggaatgtg taaatgctga
    19021 gacaggagaa ctggccagaa gtgatcatgg ctttatatgc actcccggaa tgtcttccaa
    19081 tgtaggagca ggagacataa ttgggagcat atggatgtat catggactgc ctatgtatga
    19141 tatggacatg gttcttcctc ttgttgaaat gaaggatttc cactggaggg gtatgcggat
    19201 tactgcagag aaggaaaacg gatgctggaa gatatgcgga aattctgtgg aaaaagatag
    19261 agccgtagtt catttctata agattgcgga aaccgctaaa ataggggatg ccgctgagcc
    19321 gaaggaagaa aatgatataa acagtggaaa agtttgtgaa gttaagcttt gtaacggtga
    19381 atctgtaatg gtgaatttgt gacagtgaat ctgtaaagat gagtttgtaa tagttagttt
    19441 gtgatggcga aattaaagta acagaaattt tggctaggtt ttagaacagg accgggcttg
    19501 gaggaaaaca gtaaggaaac aggcagggag gaaaatagga aggaaacagg caggtagtat
    19561 aacagcaaag aatcggggca ggaggaaaca gaaaatgacc ggtattgata atgaaaaagc
    19621 agttgagaat aaaataggta ctgattacga gcctaccgcg aataaagcaa ctgatagtgt
    19681 aaatgctgat aatatgactg atgataatgc ggtagctgat ggttatgctg ttagtaatat
    19741 agctgccacc agtgtaaagg aaaaagaaaa agaaatcggt caaatgattg acatggtgct
    19801 gaaaattgaa aaggaaattt caaagcacat gattggccag aaagagttga taagacaggt
    19861 agtaatagct atcctttgcg gaggaaatgt tcttcttgaa ggagtaccgg gtcttggtaa
    19921 aacaaggctt gtgaaaacta ttggcagggt gctggatttg aagttttcaa ggatacaatt
    19981 tactccagac cttatgcctg ctgatgtaat gggcactaac atcattatta aatcaagtga
    20041 aggtgaaggg ggattccgct tccagaaggg gccggtattc agtaatattg tccttgcgga
    20101 tgagataaac agggcaactc ccaaaactca gagtgccctg cttgaggcca tgcaggagca
    20161 gacagtaacg gcaggtggga ccacatattc cctgcctcag cctttctttg tgctggctac
    20221 acaaaacccc attgaaatgg aaggcacata ccccttaccc gaagctcaga tggacagatt
    20281 cctttttaag cttattgtac cattcccatc cctgcaggag ctttctgaaa ttgttaccat
    20341 gaccacagga ggaccggaaa aagagttgag agcagtctgc agtggcaatg atattatgtc
    20401 aatgaggaac attgctgccg aaattcctat agcaaaacct gtcctggagt tcgctatgaa
    20461 actggtgctg gcaactcatc ctgaaagtcc atatgctact gaaaaaaca
//
LOCUS       DAOSDD010000058        19678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1157_length_19678_cov_5.333741, whole genome shotgun sequence.
ACCESSION   DAOSDD010000058 DAOSDD010000000
VERSION     DAOSDD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19678
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1157_length_19678_cov_5.333741"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PK733_07115"
     CDS             complement(<1..173)
                     /locus_tag="PK733_07115"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ10348.1"
                     /translation="MSLIFYLSHQPAAKSNELSTGITEVIINTIETVAPQAKIEKGDL
                     HHIVRKNAHFFYY"
     gene            complement(630..1853)
                     /locus_tag="PK733_07120"
     CDS             complement(630..1853)
                     /locus_tag="PK733_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10349.1"
                     /translation="MKNSIEVRKITDGKGYYFKPYYDMQPWSKSGRFFICMRSKFQER
                     PPQPGDELELGVIDLEEKNKFIPVAFTRAWNFQQGCMPHWLPGYEDSKIIYNDIINNE
                     CFSFIVDISTGISTDKKEVFSLPIQAISYSRNIGASLNYARWGYERPGYGYAGIKDPF
                     HGINQPENDSVHIIDLKTGKTEPVMFLKGVAALTSDNDRRKGSFMYFCHLIFTPDGKS
                     LTGICRWWCPDIVDEVYKPKINIDGAVPERRHCLWGYDIDENKPYIVINDGLVSHASF
                     KDDENIIVYASRRCYEPAAYHLINIYTKEYRVIGKDLLSCDGHMSYHKNKELLLTDTY
                     PDDEYIRSLKIYNEKEDKEIKLGRFYAPPDLMGELRCDLHPCWSRDYMDIAIDSIHEK
                     GNRNMYLVSFDQDSV"
     gene            complement(1863..2876)
                     /locus_tag="PK733_07125"
     CDS             complement(1863..2876)
                     /locus_tag="PK733_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10350.1"
                     /translation="MLKVAVAGAGKAGTILTEAFYLNPSAEVTRIVSRTKESAEKLAL
                     KFDVKNYGTDFNEIINDDNVDAVVIASPDKYHYSQAMSAFNAGKHVLCEKPMCRSVSE
                     AEDMINASKKAGVILMVCFTERFNQPCLEAKSRIDRGEIGDPVMLLVRRCHPKFIVRG
                     RKWLNDEETGGVLNYAGTHNIDLACWLMGSEPNRVYAEMGQLVLENQNFTDSVVLTLS
                     FKNGAIASLYESFAYPSPYPHGVDRSIEVLGKMGCIKIDFMNQPLSVYSNEGMNIADS
                     QTWPQVDGSIMGGAIVNEVNHFIKAVTKKEKVLTSGEDALIALKIAHAAMEAYLTGKA
                     VYL"
     gene            complement(2897..4027)
                     /locus_tag="PK733_07130"
     CDS             complement(2897..4027)
                     /locus_tag="PK733_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10351.1"
                     /translation="MSKNEQLNIGVVGLGVRSRILLSPILESEYARLAAVCDLNMEAM
                     DRTLKDFKDVKRFDSYEEMLDKGNIDAVILGTPMQMHAPQAIRALERGINVYSEVVAA
                     VSMEQCRELVIACNKSKAKYMLGENFNYMKMVMIVKNIIESNILGEPYYAESEYATNA
                     RGMASDGTWRNEWMFGVNGITYGMHNLGPLLTWFKDDRVVRICCEGSGNHYKKPDGNP
                     IVLEDSCVMLAKTEKDRLLKIRIDLQGSRPRLMKFSLQCTKGMFDSARSESENDIIWM
                     EDIGDWSPKSYMDLDKFEGKHLPDIWRKYGEAAGRLRYGGRDFDYIIMMDFIEALYNG
                     RRMPVGIHEAMDMTVPCLLSQESIKLDGKWIEVPDSRTWQAL"
     gene            complement(4103..5608)
                     /locus_tag="PK733_07135"
     CDS             complement(4103..5608)
                     /locus_tag="PK733_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10352.1"
                     /translation="MKRKISLIIVCVLVFTFILSACTVAGTKSGTTSSDTDTTLQDTK
                     PAEKIKITTAQFLEQSTEDYSINENPYVDFVEEKFNIDLTIIRTSSGQYGEKLNALLA
                     AGDIPDLFISNKTFIAKYAEDGIAMNIDSLKPLAVNLNKNVNEEAWQAAMYNGKLYAI
                     PNQRYDPFPYLSFVNKNFVETLNIDVNNIKTIDDYYDMFVKFRDNFENSIPVTAFINW
                     PLMPLIDAFDAFSYKVIDGELLPNYLQPQYKEWLKFMQKMYKEKLIDQEFITQQSEQT
                     WSKINSGKVGFYSWFWHQDVMVNQGGSRDDWLPIKPALKADGIESKLIYHSPARQFFF
                     IGSKSKNPEKVIEMLDWAASEEGQLFIQAGLEGWDYDVKNGEIVMREGRKGQNMSWRF
                     VTIGMDPPKLTSEKVLGVFAEIYGESGVRNYQAAYEFGGYDPILLNIPSFPDLDKYDF
                     NKMRTEFDFNAIIGNIGIDAQWDSHITTFRKNGGDLLIDSLTKWYNNDYKK"
     gene            complement(5676..6554)
                     /locus_tag="PK733_07140"
     CDS             complement(5676..6554)
                     /locus_tag="PK733_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10353.1"
                     /translation="MDLQINKKTSFDYFNILFLFMYGIICIFPFYYVFIVSISDFSQV
                     TNNIVFIWPKGVNLGIYKNIMRYKGFTDGLKVSVFRTVVGTALGLSLQTMLAYALSKK
                     CLPGRRFMVLYIIFTLIFNGGMIPTYIVIRNLGLVDKIWVLIIPGLISSWNVILLMSF
                     FSSIPVEIEESARIDGANDILILIKLILPLSLPSLATIGLFIAVGHWNALMDGVIYIN
                     REELKPLQAFLVNLVSSQNSSMGMNHISFSEEQRLPPYTTRCAIIFASTVPILVVYPF
                     VQKYFIKGLMIGAIKG"
     gene            complement(6565..7473)
                     /locus_tag="PK733_07145"
     CDS             complement(6565..7473)
                     /locus_tag="PK733_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10354.1"
                     /translation="MKNFMKKILYYKYLYILAIPGLLLVFIFSYLPMGGLIIAFKDYK
                     FLKGIFGSPWVGLENFKFIFTQFPDFYNIVFNTLIISVYKKIFITPFPIILALLINEL
                     RNLRYKKLVQTTIYLPHFVSWAIFGTIIIQFLSPEMGLINGVIKSLGFEPIYFLTESR
                     YFRTIVVLSELLKESGWSTIIYLAAISNIDTETYNAAIIDGANRFQRAIYITVPGISD
                     TIVFLFLLSLGQILNVSFDQIYVLLNPLVYDVGDIISTYVYRIGIGGAKFSLATAIGL
                     FQSFVGMTLIITSNQLSKKLFDKSIW"
     gene            complement(7595..9934)
                     /locus_tag="PK733_07150"
     CDS             complement(7595..9934)
                     /locus_tag="PK733_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10355.1"
                     /translation="MRRIMFEKKLPKKLFKKSLFSYFIILLIPIVIIGIIYLYVLGVV
                     TENSIKFRENMLEQTRNAIDLQFSEIENLVIRLNSDDRINYLAAMNTEEYWKDSFQVY
                     ETSLELAKYKSIRAFVDEIYIYFLKGKYALSEAGSLNADLFYNMYFESSRVNREVWND
                     VINNYNEKQYLSLSYLSHLSGDVTTENNPDRAVFIQSIPIGYKSRYEAKAVVFMNMES
                     LKGYLQKNNTTSSCFTYILSNGKIIASSNPETSKYSTELDVQIKDNLGYVIKDIAGEK
                     MLVTYSSSYNNEWVYVSVMPTYEVTNQVKVLRNIFTIACILCFFLGISGAIVFTYHNY
                     KPIQQAIKDLVEYTKESFEEMDSNFINYSVNNLINENVKMKDRIEEYFPYIKNEFLLN
                     LIYGRMNHKEIITIAGQLNIALDNRCFTVVIYTLSRYERSYSADILRELFVSKIAVED
                     QIKNICDCYYGLELDNDKVAFLLIFNKIDENYSRYIKEKIEQITKKLHETNKIKLRFS
                     IGDVVHDCNEIPTSYRKAKHLMDYSIIVNNKESVLTEIQRIEGMYYYPIDVENSLITM
                     AKAGDYNGVIKLLDGIYSYNFVKTELGIDIIMCLYEEIFHTLIKTITYVFAEEKDKAG
                     EMIKKCKDIDYMRKPLNVFDRYKEVFGELCMYSNDKKVSHNDRMKTRILEYLDIHYRD
                     NNISLAVIADYFEYSEVYFSCFFKEQIGENFSTYLNRMRLKKACELLKQTEQEINEIS
                     AATGYTNSHIFRRTFKKYFGVTPSDYRSIPELHSFRFTD"
     gene            complement(9953..10933)
                     /locus_tag="PK733_07155"
     CDS             complement(9953..10933)
                     /locus_tag="PK733_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10356.1"
                     /translation="MKTVLIGCYDHVGVFLNALKEKGIILSAFAPGGADENYREFYEK
                     HLSDTGVKFYNNYMEMLEKEKPVLAGVCPIVYKIGKITCEVLRKGINCISEKPLALTL
                     DEVENIKEAQSKTGAKVISMLEMRYHKPFYTLHKMVSKGVIEEPLMVSVQKSYKIGNR
                     ASHFSKRETYGGTIPYIGSHIFDLTRWISGKELESVYAYHTNKGNNNNGEMEAIVSCL
                     FKLKGGGTAVNHIDYLRPATAKTWGDNRMRIAGTRGVIETIDDKVTLIDETNVSKEIE
                     QEEVGSPFVDFITMLEEGGSSILNTADCIKIAEICIRSRESADKGIVVTL"
     gene            complement(10934..11962)
                     /locus_tag="PK733_07160"
     CDS             complement(10934..11962)
                     /locus_tag="PK733_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ10357.1"
                     /translation="MMKKVIINGVRSTDIVKVPVPEPKEDWALVKIVASPMCTEYKSW
                     IEGTIIRSLGHEAAGEVVEVAQPCKVKPGDRVVLLSRFPCGKCNYCVSGNHVFCRNNY
                     NYAQFSNTGENFATMAQYILKPSWLLKPIPDNVSYEIASLACCGLGPAFGASEEMKIG
                     AFDDVLVTGCGPVGLGAVIVNKYRGARVIAAEPNEWRRKRALELGADAVIDTGEEGAL
                     EKIREFSEDGIGVSAAIDCSGNTKAEKLCIDATRPKGRVAFVGQGKGLTINVMSDLLL
                     KGLTLHGIFNYNVNDYPKVMRIIKNYDIINLLISHVYPMSQVQTAMETLESQNCAKVI
                     LKPWEGEY"
     gene            12180..12338
                     /locus_tag="PK733_07165"
     CDS             12180..12338
                     /locus_tag="PK733_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10358.1"
                     /translation="MDILEDFREDGDRVIQRYIEIHKLHKEREHYIYHASHETLDIRD
                     KKWTEVWL"
     gene            complement(12454..12700)
                     /locus_tag="PK733_07170"
                     /pseudo
     CDS             complement(12454..12700)
                     /locus_tag="PK733_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611792.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            12942..13073
                     /locus_tag="PK733_07175"
     CDS             12942..13073
                     /locus_tag="PK733_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10359.1"
                     /translation="MGFPIQVGAMDYGIELEAIIGLDMFTRCNKAVLDLNDFTLRRT"
     gene            complement(13408..13560)
                     /locus_tag="PK733_07180"
     CDS             complement(13408..13560)
                     /locus_tag="PK733_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10360.1"
                     /translation="MFNFSANNLIFVSKSQYDKCNILIMKVNSADKIYLVLEMIKEDK
                     KNVLKP"
     gene            complement(13768..14106)
                     /locus_tag="PK733_07185"
     CDS             complement(13768..14106)
                     /locus_tag="PK733_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10361.1"
                     /translation="MKQESRGGKNIAFECINKESKKVERWKGYISSFEDYGTHYEIRI
                     VSRSSILVLFGRTTQGAFGCMPDFGAGCHLVNPRDKFWNTEKLTRVLGKVDGIIVATA
                     LYTIADKLKI"
     gene            complement(14090..14329)
                     /locus_tag="PK733_07190"
     CDS             complement(14090..14329)
                     /locus_tag="PK733_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10362.1"
                     /translation="MQGLSAEETIEYCRSRMRIAGCTSEVFSPSALAAIHSVSGGFPR
                     NINNIAVATLMYCTEKKMLTADEEAVYQANIETGI"
     gene            14556..15344
                     /locus_tag="PK733_07195"
     CDS             14556..15344
                     /locus_tag="PK733_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10363.1"
                     /translation="MDISYWRELNDGFTDFLVEKGYTHDITRHYRGTINLLIRFANAN
                     GYKAYTQEIGEKFLEFEERLMYLQQAGYQRQRLVIRRFNKYLDDGKYSYTYLRVNYEC
                     PEAFKEVLEHYLKSLETANLKQCTIDKHRVFTIKLCRDFAKNGIESWDAVDAKALSGA
                     FDRATSKAQFAACIRKLFGFLVKEGIVKYNYAELPQNIFVNLESSLTTGTIAHMPSGC
                     HSQAASQRKMCLIKRLFPGGSRPLRANQSLRGIMQATCAGLLDT"
     gene            complement(15713..16069)
                     /locus_tag="PK733_07200"
     CDS             complement(15713..16069)
                     /locus_tag="PK733_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10364.1"
                     /translation="MTAHKDSLNVIGNDNYNDNNGNYNSNGNYTDNDNDNSNYNCTDK
                     GIGNGIGTTKPGTKYNEIEKKWLFKQVLGSAVVFLARHQMQRIWDTGLLQDLREPEPK
                     RFSQVWTPFYADALKQ"
     gene            complement(16359..17442)
                     /locus_tag="PK733_07205"
                     /pseudo
     CDS             complement(16359..17442)
                     /locus_tag="PK733_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850186.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
     gene            17678..>19678
                     /locus_tag="PK733_07210"
     CDS             17678..>19678
                     /locus_tag="PK733_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ10365.1"
                     /translation="MVTYFFRKRKSVVYSWMLSYILIILIPVATTSLVYFISKNIIED
                     EVKKSNEKTIVKIRDNMDSLLNDFNRFSIEVAKNQALQNVFTLNKSSADYNYRLLKAA
                     KDMNTYKIFNSAYIRFYIYINNLNTVIGQGIVYDQETYYRLYVSSIESNMEKWLEAMN
                     MKHYGECMFLPYIGYQKTDAEAAAFVWSIPYDVIGNFSANLVLLFDLEEFVSGKENEL
                     YNNDRNLYVLNKNGALVAGNSLNDIQFDFIYSKIDKYQGNLVENVNGKKTVITYTTSK
                     INGWKYITLTPQEVFWNRVQFIRNLMIGGLILCIFLGGFISYFFVKKNYNPLKETINY
                     FKEKFHSEHNWENDEYGFIRNALGETINLKEEIQTKLEEQNRILKSYFIVNLLKGKEL
                     FAPFDELLTTYDINFRYDNFTVMAIYIEHLDETFWNKHLEKGVNKFEIAKYSISNVLD
                     KLVNRDNYGYIVEVDDILVCLVNLNAGSETYKHELIKLVDEAKGFLEKNYCIKITTAI
                     GALHNGWEGIPDAFTEAMQTLEYSRVIGTCNTSFYNEISWDTGSYYYPAEKEYKLINC
                     IKSGDLIEACNILEDIFNKNFNSATPSIDIAKCLMMDLVGTVLKATNKNNKKDNNDFD
                     EKFIPVREIIECCTLLEMKKKIFEIVENICEYTSTDNCHTNFQ"
BASE COUNT         5977 a         3879 c         3195 g         6627 t
ORIGIN      
        1 aaataataaa agaaatgagc gttcttcctg actatatgat gaaggtctcc tttttcaatc
       61 ttcgcctgtg gggcaactgt ttctattgta ttgataatga cttctgttat gccagtactt
      121 aattcattag atttagctgc tggttgatgt gataagtaaa aaatcaaact catccaaaga
      181 attacagcta tcgtggttaa atggactttt tgaaggggac accttattca attaaaattc
      241 catttatata gacaggctga aaattttcta actttcaaca aacttctttc ctgttttttc
      301 tgtattaagt tcttctaagt tacattatat aaattatata taaaatataa atatttttca
      361 tattgttatc ctgcaaatta ttaagatcat attctatctc aaattctttt aattttcttt
      421 aacaacattt aaaattaatt ccctcaactt tcatatatgc attatccaca acaacatttt
      481 caaaaggaac catcgacacg tccagattcg agtataaatg caaatctgct tctttttgca
      541 tgttctatta tctacagact ttatgttgta gaaccaattt ccttatcact gtttattaaa
      601 aacgatatgt taagcattat tatcctaaat catacactat cttgatcaaa acttaccagg
      661 tacatattcc tattcccctt ctcatgaatg gaatcaatgg caatatccat ataatcccta
      721 ctccagcaag gatgaaggtc acacctcaat tctcccatta aatcaggagg agcataaaac
      781 cttcccaatt taatctcctt atcctctttt tcattataga tctttagcga ccttatatac
      841 tcatcatccg gatatgtatc cgtaagcaga agctctttgt tcttgtgata gctcatatga
      901 ccatcacagc ttaacaagtc ctttcctatt accctgtatt cctttgtata tatatttata
      961 agatgatatg cagcaggttc ataacacctt ctgcttgcat atacaataat attctcatca
     1021 tccttgaaag aagcatggga aaccagtccg tcatttataa ctatatatgg tttattctca
     1081 tcgatatcat aaccccacaa gcaatgtctt ctttccggta ctgccccatc tatatttatc
     1141 ttgggtttat atacttcatc tacaatatcc ggacaccacc agcggcaaat tcccgttaac
     1201 gacttcccgt cgggagtaaa tataaggtgg cagaaataca taaatgagcc ttttctcctg
     1261 tcattatcgc tagttaatgc cgcaactcct tttaagaaca taaccggttc tgtctttcct
     1321 gtttttaagt ctattatatg tacagaatca ttttccggtt gatttattcc atgaaaggga
     1381 tccttaatac cagcataacc ataacctggt ctttcataac cccaccgagc ataattaaga
     1441 gatgccccaa tatttcttga atatgaaata gcttgaatgg gtaatgaaaa cacctccttt
     1501 ttatcagtac ttataccagt gcttatatca acaataaagg aaaaacattc attgttaatt
     1561 atatcgttat atataatctt gctatcttca tacccgggca gccaatgggg catgcatccc
     1621 tgctggaaat tccatgctct tgtaaaagct acaggtatga atttattctt ttcttccaga
     1681 tcaattactc ccagctccag ctcatctcca ggttgagggg gacgttcctg aaatttgctt
     1741 ctcatacata taaaaaagcg tcctgattta ctccagggct gcatgtcata atatggttta
     1801 aagtagtacc ccttaccatc tgttattttt cttacttcaa tactgttttt cataaaaccc
     1861 tcctataaat atactgcttt tcctgtaaga tatgcctcca ttgctgcatg agcaatcttt
     1921 aatgctatta atgcatcttc tccggaagtc aaaacctttt ccttttttgt aacagctttt
     1981 ataaaatgat taacttcatt aacaatggct ccacccatta tactcccatc gacctgaggc
     2041 caggtctggg aatctgcaat gttcattcct tcatttgaat aaactgacaa aggctgattc
     2101 ataaaatcaa tctttataca gcccatttta cccagtactt ctatacttct gtccactcca
     2161 tgaggatatg gagacggata ggcaaaactt tcataaaggg atgctattgc tccgttttta
     2221 aaacttagag taagaacaac agagtcggta aaattctgat tctctagaac cagctgtccc
     2281 atttccgcat ataccctgtt aggttcacta cccattaacc agcatgctag atctatatta
     2341 tgggtaccgg cataattcaa tactccccct gtttcttcat catttaacca ttttctaccc
     2401 cttacaataa acttagggtg gcatctcctt acaagaagca ttacaggatc tcctatttct
     2461 cccctgtcaa ttctgctttt tgcttcaagg caaggctgat tgaagcgttc cgtaaaacaa
     2521 accattagaa taactcctgc cttctttgaa gcattaatca tatcctctgc ttcagaaaca
     2581 ctcctgcaca tgggtttttc acatagtaca tgcttacctg cattaaatgc agacattgcc
     2641 tgggaataat gatacttatc aggggatgct attaccacgg catccacatt atcatcattt
     2701 attatttcat taaaatcagt accatagttc tttacatcaa acttcaaagc aagcttttct
     2761 gcactttcct tggttcttga tacaatcctt gtgacttcgg cactaggatt aaggtaaaaa
     2821 gcttcagtta atattgtgcc tgctttacct gctcctgcca cagcaacctt taacatagaa
     2881 tcacgctcct tttaagttat aaagcctgcc atgttctgct gtcgggtact tcaatccatt
     2941 tcccgtcaag tttaatagat tcctgactta acaggcacgg tacggtcata tccattgctt
     3001 cgtgaatacc aaccggcatt ctgcggccat tatacaatgc ttcaataaag tccatcatta
     3061 ttatgtaatc aaagtccctt cccccatacc ttaatcttcc tgcagcctca ccatattttc
     3121 tccatatatc aggaagatgc ttaccctcaa atttatctaa atccatgtag ctcttaggac
     3181 tccaatctcc aatgtcttcc atccatatta tgtcattttc actttcgctt ctggcagaat
     3241 caaacatacc ttttgtacat tgcagagaaa acttcatcaa cctcggtctg ctgccctgta
     3301 agtcaatacg tatctttaag agtctgtcct tttctgtttt agccagcata acacaggaat
     3361 cctcaagaac aatcggatta ccgtcgggct ttttataatg attgccactg ccttcgcagc
     3421 aaatccttac taccctatcg tctttaaacc aggtaagaag tggacccaga ttatgcattc
     3481 catatgtaat tccgttaaca ccgaacatcc actcgtttct ccatgtgcca tcagatgcca
     3541 tacccctggc gttagttgca tattcactct cagcatagta tggttcccca agaatattac
     3601 tttcaattat attcttcaca atcataacca ttttcatata attaaaattc tcacctaaca
     3661 tatattttgc tttagactta ttacaagcta tcaccaattc cctacattgt tccatagaaa
     3721 cagctgcaac aacctcacta tatacattaa ttcctctttc taatgctctt attgcttggg
     3781 gtgcatgcat ttgcatgggc gtaccgagta taaccgcatc aatattgccc ttatcaagca
     3841 tctcttcata actgtcaaat ctcttaacat ccttaaaatc ttttaatgtt ctgtccattg
     3901 cttccatatt taaatcgcaa acagctgcca gtcttgcgta ttcactctca agtatgggcg
     3961 ataaaagaat tcttgatctc actcccaatc cgacaacccc aatatttaat tgctcatttt
     4021 tactcaaatt aaccttcctc cctttcataa aaaaatgtac aaggccgatt acgtgaacgg
     4081 accttgtact aattcctctc gcttattttt tataatcgtt gttgtaccat ttagtcaggc
     4141 tatcaattaa caaatctccg ccattctttc tgaatgttgt aatatgggaa tcccattgag
     4201 catcaatgcc aatattacca attattgcat tgaaatcaaa ctctgttctc atcttgttga
     4261 aatcatattt atccagatca ggaaatgagg gaatattaag cagtatagga tcataacctc
     4321 caaactcata ggctgcctga taattcctta caccagattc tccatagatt tctgcaaata
     4381 cacctaatac tttctctgag gtcaatttag gaggatccat acctattgta acaaatctcc
     4441 atgacatatt ctgccctttt ctgccctctc tcattacaat ctcaccattt ttaacatcat
     4501 aatcccaacc ttccaatcca gcctgtataa ataactgtcc ctcttctgat gctgcccagt
     4561 caagcatctc tattactttc tccgggttct tgctcttgct ccctataaag aagaattgtc
     4621 ttgcaggaga atgataaatc agttttgatt caatgccatc tgcttttaat gcaggtttta
     4681 ttggcaacca gtcatcacga cttcctccct gattaaccat tacatcctga tgccagaacc
     4741 aagaatagaa tccaaccttt cctgaattaa ttttactcca ggtctgttca gactgttggg
     4801 taataaattc ctgatcaatt agcttttcct tatacatttt ctgcatgaac ttcaaccatt
     4861 ccttatactg aggctgaaga taattaggta gtagttcacc atcaattacc ttataactga
     4921 aagcatcaaa agcatcaatt aaaggcataa gaggccaatt tataaatgct gttacgggta
     4981 tgctgttctc aaaattatca cggaatttaa caaacatatc ataatagtca tcaatggtct
     5041 taatattatt tacatcaata ttaagagttt ctacaaaatt tttgtttaca aaagacaaat
     5101 aaggaaaagg gtcgtaacgc tgattgggta tggcatacag ctttccatta tacatagcag
     5161 cttgccatgc ttcttcattt acatttttgt taagattaac tgctaaaggt tttaaactat
     5221 caatattcat ggctattcca tcttctgcat atttagcaat aaatgttttg ttgctaataa
     5281 agagatccgg aatatctcct gcagcaagaa gtgcgttgag tttctctcca tattgaccag
     5341 aactggttct gattattgtc agatcaatgt tgaatttttc ttcgacaaaa tcaacatagg
     5401 gattttcgtt gatactgtag tcttcagtac tttgttcaag gaactgtgct gttgtaattt
     5461 ttattttctc tgctggcttg gtatcctgca aggttgtatc agtatcactt gacgtagtac
     5521 cggattttgt tcctgcaacc gtacaagccg aaagaataaa tgtgaatacc aaaacacaca
     5581 caattattaa tgatattttc cttttcatct taaaacacct cacattttta tttagaacag
     5641 acttgataag gctcgcttat cccacaaggc tgttactatc ctttgattgc tccgatcatt
     5701 agtcctttta taaagtattt ctgtacaaag gggtacacaa caagaatggg cactgtggaa
     5761 gcaaatatta tcgcacatct tgttgtataa ggaggaagtc tctgttcttc ggaaaaagag
     5821 atatgattca ttcccatgga agaattctgc gaagaaacca gattcacaag aaatgcttgc
     5881 agtggtttta actcctctct gtttatgtag attaccccgt ccataagagc gttccagtga
     5941 ccaaccgcta taaataatcc aatggtggca agggaaggta gtgataaagg caatattagt
     6001 ttaatcagta tcagaatatc atttgctcca tctatacgag ctgattcttc tatttccaca
     6061 ggtatggaag agaaaaagct cattagaaga ataacattcc aagaacttat aagtccgggt
     6121 attatgagta cccaaatttt atctactaga ccaagattcc ttatgactat gtatgtaggt
     6181 atcattccac cgttaaatat gagggtaaat attatgtaca gaaccatgaa ccttctacct
     6241 ggtagacatt tttttgaaag tgcatatgcg agcattgtct gtaatgataa acctagtgca
     6301 gtcccaacaa ctgttctgaa aacacttacc tttagtccat cggtaaaacc cttataccgc
     6361 attatatttt tatagatacc taaatttact cccttaggcc aaataaagac tatgttgttt
     6421 gttacctggc tgaagtcgct tatggatact ataaatacat agtaaaaagg gaatatgcaa
     6481 attataccat acataaacag aaacaatata ttaaaatagt caaaggaagt ctttttgttt
     6541 atctgtaaat ccatatttgt cacctcacca tatactttta tcaaacagct tctttgacaa
     6601 ctgattgctt gtaataatca aggtcattcc cacaaaggac tgaaacaaac ctattgcggt
     6661 tgccagactg aacttggctc cgcctattcc tattctgtaa acataggttg atattatgtc
     6721 acccacatca tatacaagag gatttaagag cacatatatc tggtcaaaac ttacatttaa
     6781 tatttgtcct aaacttaaaa gaaacaggaa tactatggta tcgcttattc ccgggacggt
     6841 tatatatatt gctctctgga aacgatttgc tccgtctatt atagctgcat tgtaagtttc
     6901 cgtgtctata tttgaaattg cagcaagata tattatcgtg ctccatcccg attcctttag
     6961 tagttcggat aaaacaacaa ttgtcctgaa atatcttgat tcggtaagaa aataaattgg
     7021 ctcaaatccc aagcttttta taactccatt tattagtccc atttctggtg atagaaactg
     7081 tattataatt gttccaaaga tagcccatga tacaaaatga ggtagatata tggttgtctg
     7141 gacaagtttt ttatatctta ggtttctcag ttcatttatt aaaagagcca gtattatggg
     7201 aaaaggtgtt atgaatattt ttttgtaaac acttattatt agtgtgttaa atactatatt
     7261 gtagaaatcc ggaaactggg taaaaatgaa tttgaagttt tcaagaccta cccatggact
     7321 accaaatatt cccttgagga atttataatc tttaaaagca attataaggc cacccatagg
     7381 aagataagag aaaataaaaa caagtaatag tcctggtatt gccaatatat ataaatattt
     7441 gtagtagagg atctttttca tgaaattttt caattctttc accgccaata cttgaatttt
     7501 catttacatc ttaagaagaa ttgcactata gaatcaaggt tctataccta atatcaaggc
     7561 tcacttctta ttatttctgc tttttcggat atttttagtc agtaaaacga aaggaatgta
     7621 actcaggtat tgacctgtag tcggagggtg ttacaccaaa gtatttttta aaggtgcgcc
     7681 tgaatatatg tgagttggta taacctgttg ctgctgaaat ttcatttatt tcctgctcag
     7741 tttgttttag cagttcgcat gcttttttta aacgcatcct gtttagataa gtggagaaat
     7801 tttcccctat ctgttctttg aaaaaacagg aaaaatatac ttcgctgtat tcaaagtaat
     7861 ctgcaattac tgcaagggaa atattgttgt ccctataatg tatatcaaga tactcaagta
     7921 ttctggtttt catccggtca ttatggctta cctttttatc attcgaatac atacataatt
     7981 caccaaacac ttccttatac ctgtcaaaaa cattaagagg cttccgcatg taatcaatat
     8041 ctttgcattt ttttatcatt tctcctgctt tgtctttttc ctctgcaaat acataagtta
     8101 ttgtttttat tagggtatgg aatatttctt catatagaca cataattata tcaataccca
     8161 gctctgtttt tacaaagtta tagctatata ttccgtcaag tagtttaatc accccattat
     8221 aatcccctgc ttttgccatg gtaatcaggc tgttctcaac atcaatggga taatagtaca
     8281 ttccctcaat tctttgtatc tctgttaaaa cactttcctt attatttact ataatggagt
     8341 aatccatcag atgctttgct tttctgtagg atgtggggat ttcattgcag tcatgtacca
     8401 catctcctat ggaaaagcgt agctttatct tatttgtctc atgcaacttc tttgttatct
     8461 gttcgatctt ttctttaata tatctactat agttttcatc tattttgtta aaaatcaaca
     8521 gaaaagccac tttgtcatta tccaattcaa gtccgtaata gcagtcgcat atgtttttta
     8581 tctgatcttc aactgctatt tttgaaacaa ataattccct tagaatatct gcagaatatg
     8641 atctctcata tctgcttaag gtatagatta caaccgtaaa acacctgtta tcaagagcaa
     8701 tgttcaactg tccggctatt gttattatct ctttgtgatt cattcttccg tagatcaggt
     8761 ttagcagaaa ttcatttttt atatatggaa agtactcttc tatacgatcc ttcatcttta
     8821 cattttcatt tataagattg ttaactgaat agttaataaa gttgctgtcc atttcttcaa
     8881 aggattcctt ggtatattct acaagatcct ttattgcctg ctgtataggt ttgtagttat
     8941 ggtaggtaaa aactatcgct cccgatatac ccagaaaaaa acatagtatg caagcaatag
     9001 tgaagatatt gcggagtact tttacctggt ttgttacttc atatgtgggc atgacggata
     9061 catagaccca ttcgttgttg tatgagctag aataggttac aagcattttt tctccagcta
     9121 tatcttttat gacataaccc aggttgtcct tgatttgcac atctagttca gttgagtatt
     9181 tgctggtttc aggattggaa gaggcaatta tcttcccgtt gctcaggata tatgtaaaac
     9241 aggaacttgt tgtgttgttt ttttgcaagt aaccttttaa gctttccata ttcatgaata
     9301 caactgcttt tgcttcatac ctgcttttgt atcctatggg tattgattgt atgaaaacgg
     9361 ctctgtctgg attgttttca gttgtcacat ctccagataa gtgggaaagg tatgaaagtg
     9421 acaggtactg cttttcgtta taattattga ttacatcatt ccatacttcc ctgttgactc
     9481 ttgatgattc aaagtacata ttgtagaata ggtcagcgtt taaactacct gcttccgaca
     9541 gggcatattt ccctttgaga aaatatatgt agatttcatc tacaaatgct ctaatgcttt
     9601 tgtatttagc cagttctaag gaggtttcat atacctggaa tgagtctttc cagtattcct
     9661 cagtgttcat tgccgcaaga tagtttatcc tgtcatcgct gttaagccta ataacaaggt
     9721 tttctatttc ggaaaactgg agatcaattg cattccttgt ttgttcaagc atgttttctc
     9781 taaatttaat ggagttttcg gttactactc ccaatacata aaggtatatt atccctatta
     9841 tgactatagg tataaggagt attatgaaat atgagaaaag agactttttg aaaagctttt
     9901 tgggtagttt cttttcaaac attattctcc tcatgactgc cctccggttt ttttataaag
     9961 taacaacaat gcctttgtct gccgattccc tggatctgat acatatttcg gcaattttaa
    10021 tacagtctgc ggtattaagt attgagcttc ctccttcttc cagcatggta atgaaatcaa
    10081 caaaaggaga tccgacctct tcctgttcta tttcttttga tacattggtt tcatcaatca
    10141 aagtaacctt atcatcaatg gtttcaataa cccccctggt tccagcaatt ctcattctgt
    10201 tgtctcccca ggttttagca gttgcaggac gtaaataatc aatatggtta acagcagttc
    10261 ctccgccttt tagcttaaaa agacatgata ctattgcttc catttcccca ttgttgttat
    10321 tgcctttatt tgtatggtaa gcatatacag attctaattc cttacccgat atccatcttg
    10381 tcaagtcaaa tatatgggat cctatatatg gtatggttcc cccatatgtc tcccttttag
    10441 aaaaatgtga agccctgttc cctattttat atgacttctg aacggatacc atcaggggtt
    10501 cttctatcac acctttactt accatcttat gcagggtata aaacggtttg tgatatctca
    10561 tttcgagcat agaaataact ttagctccgg ttttgctttg tgcttcctta atattttcca
    10621 cttcatcaag agttaaagcg agaggttttt cgcttataca gtttattcct tttctcagta
    10681 cttcacaggt tatttttcct attttatata ctatgggaca taccccagca agaacaggtt
    10741 tttctttttc aagcatttcc atatagttat tataaaattt tacaccagta tcacttaaat
    10801 gcttttcata gaattccctg tagttttcat ctgctccccc gggagcaaat gccgatagta
    10861 ttattccttt ttcctttaaa gcattcaaaa acacacctac atgatcgtag catcctataa
    10921 gtacagtttt catctaatat tcaccctccc aaggttttag tattaccttt gcacaattct
    10981 gtgattccag tgtttccatt gcagtctgaa cctgactcat gggatataca tggcttatga
    11041 gtagatttat tatatcatag tttttaatta ttctcatcac ttttggataa tcgttcacat
    11101 tatagttgaa tattccatgg agtgtaagac cctttaacaa caggtccgac attacattta
    11161 ttgttaagcc ttttccctgt cccacaaaag cgactcttcc ttttggcctt gttgcatcta
    11221 tacatagttt ttctgctttt gtgtttccgg aacagtctat ggcagcgctt actcctatac
    11281 catcttcgga aaattccctg attttttcaa gtgctccttc ttctcctgta tcgattactg
    11341 catctgcccc taattccaga gctctttttc ttctccattc atttggttca gctgctataa
    11401 ctcttgctcc cctgtatttg tttacaatta ctgctccgag tcctactggt ccgcagcctg
    11461 ttacgagtac atcgtcaaat gctccgattt tcatttcttc cgatgcacca aatgcaggtc
    11521 ccagaccaca gcaagccaaa gatgctattt catatgatac attatccggt atgggtttta
    11581 ataaccatga tggttttaat atgtattggg ccattgtagc aaagttttct ccggtattgc
    11641 taaattgtgc atagttatag ttgttgcggc agaacacatg gtttcctgat acacagtaat
    11701 tacatttacc gcaggggaat cttgatagta gcactaccct atctcctggt tttaccttac
    11761 atggttgcgc aacttcaaca acctctcctg ctgcttcatg tcctaaagaa cgaataattg
    11821 tgccctcaat ccagctctta tactctgtgc acatgggaga tgctacgatc tttacaaggg
    11881 cccagtcttc ttttggttca ggtacaggta ctttaacaat atcagtactc cttactccat
    11941 ttattattac ttttttcatc atttaaaagt ctcctttaca agtattccta ctttaatact
    12001 atataagcag tattctctgc tcaaggttct atattttata tcataggccg attatgaata
    12061 ttatcaataa aacatagttt atctttgact gacttttatc ctcgcttcat aataaaagca
    12121 tccataagaa tgctcatacc taatcaagaa atgaatacga taaaaggata gtagagaaaa
    12181 tggacatttt agaagacttc agagaagatg gagacagagt aattcaaaga tatatagaaa
    12241 tacataaact ccacaaggaa agagagcact atatttacca tgcttctcat gaaactctgg
    12301 atataaggga taagaaatgg acggaggtat ggttataatg gctaagatta agtttaaaga
    12361 tgggctaaca tatgcaggtg ctactctctc tctctgtaat aagaatgtaa caataaagag
    12421 ggctcttgta tatacaagtt cgataactac agttgcaacc tcacaaagaa tacttttaat
    12481 atatggatta cctctattga tatatgttcg tttttttacc tgcactttcg ttgttaccag
    12541 aacttaagcc tacccagaaa caaatatgct cagtagtttt aaaacagctc atgtcaattc
    12601 ctatttcagc gattattgca gatgcagctg ttttatccat tcaaggaatg ctatcaatga
    12661 tttcaatctg atgttcaaac ttctgtatta ttgcgagaac tttgctacac gtgctaccaa
    12721 aaggcgcaac aatatgctgt gctcgaaggc aattagttta cattgaaaag cggggtacaa
    12781 atcctccaat gatcagtcaa actctaccct gtcgcataaa tcattgccat aaaccatgca
    12841 acaaaggcat agcaaaattc atttttattc gaggctgtga gagccatttc ataagtcgtt
    12901 gaaaagaaaa tatattctgt tccttggatg caatagcggt catgggcttc cccattcaag
    12961 tcggagctat ggactatggc attgagttgg aagcgattat agggctagat atgttcacaa
    13021 ggtgcaataa ggctgtactt gatttaaatg actttacatt aagaagaaca tgagtaattt
    13081 gcggtaattg cttgaagtat taaaattgag agtatcactg ataatttata tcatagcata
    13141 gggttttaat gattgttgat gatatctcat acatagcttt cttaaaatca aattatctat
    13201 ctgctaatgc ttaggaaatt ttattcatta aattacctat ttaaagaagg aatttcaata
    13261 tagtatgtag aaaatacttt taatagacat gaaacgtgaa acatatatgt agttttacaa
    13321 ttattgttta gcttaggtag taaaaggatt tattaatggt tcttaaatga tgaggaggtt
    13381 ttacttgaat actggattaa ttggaaatta cggtttcaac acattctttt tgtcttcttt
    13441 tatcatttca agaacaaggt atattttgtc agcagaatta actttcatga ttagtatatt
    13501 acatttatca tactggcttt ttgatacaaa tatgagattg ttcgcgctga agttgaacat
    13561 aatattccct ttcatataaa aacagttata agattttagt actgcatcat tattagtact
    13621 gcatcattaa aggatgtata tcctttagcc tggttgtaga gttaggtgtt gcatccatgc
    13681 agagcgcttt atcgacacag agccaagcct cagttcaaat taatagaatt gggttgtttc
    13741 gcgataaatc atcaaatcca gggttcccta aatctttaat ttatcagcaa tggtatataa
    13801 tgcagtagca actataatac catccacttt ccctaaaaca cgggttagtt tttcggtatt
    13861 ccaaaattta tctcttgggt ttaccaaatg gcaccctgca ccgaagtcgg gcatacaccc
    13921 aaaagcgcct tgagttgttc taccaaacag aactaaaatg cttgaacggc ttacaatcct
    13981 aatttcataa tgggtaccat aatcttcgaa agaggaaatg taccctttcc atctttctac
    14041 ttttttactt tccttattta tacattcgaa agctatattt ttaccacccc tagattcctg
    14101 tttcaatatt agcctgataa acagcttctt cgtctgctgt cagcattttc ttttctgtac
    14161 aatacattag ggttgctact gcaatgttgt taatattccg tgggaatcca ccggatacac
    14221 tgtgtatcgc tgccagggct gatggcgaga atacttcact ggtgcaccct gcaatcctca
    14281 ttctgcttct gcaatattct atagtttcct cggcagaaag accttgcata gagtatctca
    14341 ttgttatcct ttggcggagt ggacaacatg tattcaatga aagcttgttc ctaatatgcg
    14401 gctgcccata tcccagcagc tatactatcc ttgattaatc gtcaaagcat agaaaaacca
    14461 atagtttctt taaaaattcc tcggataatt tctgtatata atctataata ctctcggaca
    14521 tcaaccgatg tcacaaatat gttttggagg gtattatgga tatcagttat tggagagaac
    14581 taaatgacgg gtttactgac tttcttgtcg agaaaggcta cacccacgac attacaaggc
    14641 actacagggg gacaatcaat ctgctgatcc gctttgcgaa tgcaaacggt tataaagcat
    14701 acacccaaga aataggcgag aaatttctgg agttcgaaga gcgcctgatg tatctacagc
    14761 aagctggtta tcagcgccag cgtttggtca tacggcgatt taataagtac cttgacgacg
    14821 gaaagtattc gtatacgtat cttcgagtga attacgaatg cccggaagct ttcaaagagg
    14881 tgcttgagca ttacctcaaa tcattggaga cagcaaattt aaagcaatgc accattgaca
    14941 aacatcgtgt atttacaata aagctatgca gggatttcgc aaagaacggc atagaatcat
    15001 gggatgccgt cgatgccaaa gcgctgtcgg gagcgtttga ccgggctaca agcaaagctc
    15061 agtttgcggc atgcatacgt aagctctttg gatttctggt caaagaagga attgtcaaat
    15121 acaattatgc cgagttgccg caaaacattt tcgtcaatct ggaatcgagt ttgacgacag
    15181 gcaccatagc gcacatgccc tcaggatgtc actcgcaagc ggcctcgcag cggaaaatgt
    15241 gccttataaa gcggttgttt ccgggtggct caagaccctt gcgtgcaaac caaagtctaa
    15301 gaggaattat gcaggcaact tgcgcaggtt tgctcgatac ttgagcgcgc ttgagatccc
    15361 tgcgtatgag ccggaattgt ttcggacaca gtcagattat gttgtgtaca ctttttcaga
    15421 tgaagagttc gcagccatta ttgacgcagc ggacaatttt gcagcaatca aaagcaaaga
    15481 aaatgagaca tcatatgcct ttccaatgct gctcaggatt ctctacggtt gcggattgcg
    15541 gctgggcgaa gcgctttcgc tgcgttggcg tgacatagat ttagatactg aagttatcac
    15601 aatacggaaa gccaaaaacc acaagcagcg aattgtccca attagcagct cattggcaga
    15661 ggtgctcaga ttatacaata ggcgcacgat tggagatgat tcggacgcca gtttattgtt
    15721 tgagagcgtc cgcataaaag ggcgtccata cctgcgaaaa acgtttaggc tctggttctc
    15781 gcaaatcttg gagcaagccg gtatcccaaa tgcgttgcat ttggtgccgg gctaaaaata
    15841 caacggcact gcccaaaacc tgcttaaata gccacttctt ttctatttcg ttgtattttg
    15901 tgccaggctt cgtagtacca ataccattgc caataccctt atcagtacaa ttatagttgc
    15961 tattatcgtt atcattatca gtatagttgc cgttactgtt ataattgcca ttattatcgt
    16021 tgtaattatc attgccaatt acgttcaatg aatctttgtg agcagtcatt attctttagt
    16081 atcctttcaa attccccata atataatttt accaaaaaaa ctataaagtt aacagtaaat
    16141 tttaatcaat taattcattt cattttgttt ttgtatttat agaaaagcca taattcaccc
    16201 tctcatgaca ctattatata ccactgtcat atattgtaaa ccatttttat tcatattgca
    16261 ataacttttg tattattttc aataaagtgt tatgagtgtt taaaaaatat atgcaatcca
    16321 tcaagaactt gcatagtgaa tttgcatatt ataaaccatt gccgtaaatc tttttggata
    16381 aaatcaaata aatctgttcc tgacttcatt tgaatgtttt gctttaacac ttcaagtgat
    16441 gtttagctgc tcttaatcaa actagaaacg attactggat catttcctac ctgcgcttga
    16501 atatccccag acgaaatgtc tttactgctt ttaccgtaac tgtgttcttt cttattattt
    16561 ggtaccggtt tcataaaatc cccccgctcc atgatggtca tcaatgcatc ttttatgagg
    16621 ggatcgtgtt gtcccatgtt atttaataac acttctctac tgtccataga aacaagcata
    16681 gggataacat caacaaaggg ccttccacca gctttaaaca taggtaccgc tgcttttaac
    16741 tgccataaag acaatcccaa tggtttcatg gggtcggtca tcattcgttg atgtcctaca
    16801 gatatataga cgtgtttttc actgtcattt gctgcaggga taggatatag agtagtgatt
    16861 ggccgacttt ggacaatata aaatatatca tcagccaaac accattcgat gtcctggggg
    16921 cagccaaaat gttcttcgat ctttttaccc acatgctcaa gctgcaaaat ctgatcatcc
    16981 ggcagcactt gctgatgcac ttaaatatct cctcgtttcc gataatgttc aaaaaagtat
    17041 cctgctgacc tgcaaaggat gctgttggta aatcctctgc agttgcactg gaacgcacac
    17101 ggcataagca tctttttcac caaactttaa gagttgatag gtgatctctt cctgtatgtc
    17161 tccagggatg gctatccctt caatggctct gtgaatctca ctgctaagtt cacttattct
    17221 atccctgttt tccgccttta aaagatatta cttaccaatt aaatcgttaa tcgacgacat
    17281 ttctctaatg attcttttaa aagcttcggt agaaatgcaa aagccctatg gtacatgtaa
    17341 tccttcaatt ttggaaagtt ccccgaagct agcaccttta ccaccaacaa gcatgattat
    17401 tgttttatca acgtcctgaa aaccaaacac ataggaattc atatgtttac ctcccttaaa
    17461 aattcttcag tctctctagc ttcgcattct atacatcttc ccggctccat ataaatccaa
    17521 gcaatatata taatgtcata atatccttaa ttttggcttg actccaatat tagttttgta
    17581 ttacgttgtt atataatata atatgtatat atatatacaa tataaaaacc ttcaaaaatc
    17641 attaagttga atataattat tattaggaga atacaaaatg gttacttatt tcttcagaaa
    17701 aaggaaaagc gtggtatata gttggatgct atcgtatatt ctaataatcc ttattccagt
    17761 ggctactaca agtttagtat acttcatttc taagaacatt attgaagatg aagtaaaaaa
    17821 atccaatgaa aaaactatcg tcaagatccg ggacaatatg gatagtttgt tgaatgattt
    17881 caataggttt agtatagagg tagcaaaaaa tcaagctctc cagaatgtat ttacactaaa
    17941 caaaagtagt gctgactaca actatagatt attaaaagct gccaaggata tgaacacata
    18001 taaaatattt aacagtgcat acatacgatt ctacatctat attaataatt taaatacagt
    18061 tattggtcaa ggaattgtct atgatcaaga aacctactat agactatatg tttcttctat
    18121 agaatccaat atggaaaaat ggctggaagc aatgaatatg aaacactatg gagaatgcat
    18181 gtttttacca tacattggat atcagaaaac agatgcagaa gcagcagcat ttgtttggtc
    18241 tattccatat gatgtaatcg gcaatttttc tgcaaatcta gtcctgcttt ttgatttaga
    18301 agaatttgtg tcaggaaagg aaaatgaatt atacaataat gatagaaacc tgtatgtttt
    18361 gaataaaaac ggcgccttgg ttgcaggaaa tagcttgaat gatatacaat tcgattttat
    18421 ctatagtaag atagacaaat atcaaggaaa tctcgttgag aatgtcaatg gtaaaaaaac
    18481 agtaataacc tacacaacat caaaaattaa tgggtggaaa tacattacac tgactccgca
    18541 agaggtattc tggaatagag tacagtttat aagaaattta atgataggtg gacttatttt
    18601 atgtattttt ttgggtgggt ttatatcata cttttttgtg aaaaagaatt acaatccatt
    18661 aaaagaaacg ataaactatt ttaaagaaaa attccactct gaacacaact gggaaaatga
    18721 tgaatatgga tttataagga atgctttagg tgagacaatc aacctgaaag aagagataca
    18781 aactaagctt gaggaacaga acagaatctt aaaatcatat ttcattgtta atcttttaaa
    18841 aggcaaggaa ttgtttgctc ctttcgatga gcttttaaca acatatgata tcaattttcg
    18901 ctatgacaat tttacagtta tggcaattta cattgaacac ttggatgaga ccttctggaa
    18961 caaacattta gagaaagggg tcaataaatt cgaaatagct aaatattcca tttcaaatgt
    19021 tttagataaa ctagtaaaca gggacaatta tggatatata gttgaggtgg atgatatttt
    19081 agtgtgtctg gtaaacttga atgcagggag tgaaacttat aagcatgaac ttatcaaatt
    19141 ggttgacgaa gccaaaggtt ttttagagaa aaactattgt ataaaaatta caacagcaat
    19201 tggagcattg cataacggat gggaaggaat tcctgatgct ttcacggaag ctatgcaaac
    19261 actggaatat tcaagggtaa tcggaacctg caatacatct ttttataatg aaatatcttg
    19321 ggacacaggg tcttactatt atccggcaga gaaggagtac aagttgataa attgtataaa
    19381 gtctggtgat ttaattgagg cctgcaatat ccttgaggat atttttaata aaaacttcaa
    19441 tagcgctaca ccttcaatag atatagccaa gtgtctgatg atggatctag ttggtacagt
    19501 tttaaaagca accaataaaa acaacaaaaa agataataat gattttgatg aaaaatttat
    19561 tcctgttaga gagataatag aatgctgtac attgttggag atgaaaaaaa agatattcga
    19621 aattgtggaa aatatctgtg aatatacctc aacagataac tgtcatacta atttccaa
//
LOCUS       DAOSDD010000059        19427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1167_length_19427_cov_5.072527, whole genome shotgun sequence.
ACCESSION   DAOSDD010000059 DAOSDD010000000
VERSION     DAOSDD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1167_length_19427_cov_5.072527"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK733_07215"
     CDS             <1..441
                     /locus_tag="PK733_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ10366.1"
                     /translation="QGQEYAWKFSALSSVTGLVLGIILAITAKFTLSFFNVSVEVYNS
                     TLWMQYMMAVIMVFRVFTIVVAVGILRGGGDAHFAFISESITMWLIGVPLAFIGAFVF
                     HLPIHFVVALSTIEELLKVYIFIRRLISKKWINNLVRDVALDVA"
     regulatory      724..839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            941..2131
                     /gene="metK"
                     /locus_tag="PK733_07220"
     CDS             941..2131
                     /gene="metK"
                     /locus_tag="PK733_07220"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ10367.1"
                     /translation="MERKLFTSESVTEGHPDKICDQISDAVLDAIYECDPIARVACET
                     AVTTGLVVVMGEITTHCYVDIPKIVRETIREIGYDRAKYGFDCDTCAVITSIDEQSPD
                     IALGVNQALESKNGEMNDVEVEAIGAGDQGMMFGFACDETPELMPMPISLAHKLTRRL
                     SQVRKDGTIPYLRPDGKSQVTIEYEGHTPVRVDTVVISSQHSQEVEHDTIEKDIIEHV
                     IKPILPVELIDEKTRILVNPTGKFTVGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSG
                     KDPTKVDRSASYAARYVAKNIVAAGLASKCEVQLAYAIGVAKPISISVDTYGTGTIPD
                     SEIVKLINSHFDLRPAAIIKMLDLRRPIYKQTAAYGHFGRTDIDLPWEKIDKAEILKK
                     DAGI"
     gene            2406..2849
                     /locus_tag="PK733_07225"
     CDS             2406..2849
                     /locus_tag="PK733_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10368.1"
                     /translation="MFIQIFNIAMYILALYGIISLIMSYVRHLWRGSGIKHLNTNMRM
                     VLIAKDQEDVIEGIIRSLYISGVSEGGAGIYVVDADSQDKTLEILQKLNRTYDNMRIL
                     AFNERDRIFDGFIEDIEALEDTEVDIGEDIEDIHHIEDLENIRQM"
     gene            2900..5497
                     /locus_tag="PK733_07230"
     CDS             2900..5497
                     /locus_tag="PK733_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10369.1"
                     /translation="MIIVEGTIEGIIFTNESNGYAVCEIAHEKGVVTLVGYMPFISEG
                     EIIKASGLWVTHPDYGEQFKVEYFEKILPNTAEAIEKYLGTGIIKGIGKATARKIVKK
                     FGDNALNVILENPEKLRDIKGMSRAKALEIGQAFAKRKNFTDIVSFLQEYGISPAYAA
                     KVYKIFGENTIEEIRANPYRLADEIPGISFKTTDRIANSLGIDPESRLRICSGLKYVL
                     IRAAVNGHTYLPYDELAKYGENLLGVSAGHISDALVFMRFDGTIYIEKENLRNNFNDN
                     FNNNYNDKFNDSLKSNIRYNAKNNTIDDYAGEHENDAESSDGRVYLSDLHQAELNITR
                     RLTALANSVLLLKIRGIDKTLEKTLKDLQQEENIILAETQKTAIKEALGNGALVITGG
                     PGTGKTTIIKSIIKIFERNKFKVALAAPTGRAAKRMTEATGYESKTIHRLLEIGYVGE
                     DNEISFMRTDVNPIDADVIIIDEMSMVDILLMDSLMKAIKPGSRLILVGDVDQLPSVG
                     PGNVLKDIISSGAIRTVRLTEVFRQAEKSLIVVNAHRINRGERPILNEKSKDFFFIPR
                     NNPTDIVNLVLDLCGRRIPSQLGYDPLRDIQVLTPTKKGLVGVGNLNIELQKELNPKN
                     KNRNEKAFPNFTFREGDKVMQIKNNYNIQWEKIQEQKDGSRQEQREGSRQEYGDGSSV
                     SHGNYASGNYLFDDNENDDNVLGDGGTGYNQLGNSQFENNETGNNQTENNQAENNGGE
                     GIFNGDTGVIRQIDHDDEKIRVVFDDNRLVEYYFDAFDEIEPAYAITIHKSQGSEFPV
                     VILPIFPGPPVLMTRNLLYTAITRARDLVIVVGLEGTLYQMIDNNRETLRYSGLADKL
                     QKSAGSGGY"
     gene            5605..5697
                     /locus_tag="PK733_07235"
                     /pseudo
     CDS             5605..5697
                     /locus_tag="PK733_07235"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
     gene            5746..6276
                     /locus_tag="PK733_07240"
     CDS             5746..6276
                     /locus_tag="PK733_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOJ10370.1"
                     /translation="MTPCRYFDRVFCVFEYSGYVREAIVRYKYFGKSHYYKTFAALLA
                     DKLKRITCSGDFNVILSVPLHKHKERKRGYNQSRLISKELSRIMQVPERSELLERIKN
                     TPAQSLLHKNERYLNIKNAFKVTDVLEVKGKSILLIDDVLTTGNTLNECSRVLKEAGA
                     KKVDIAVIAVAYTFEL"
     gene            6367..6852
                     /locus_tag="PK733_07245"
     CDS             6367..6852
                     /locus_tag="PK733_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ10371.1"
                     /translation="MADLRNCNRCGKLHNFIGGSLLCPSCRDEDEADFQKIKRYLYEN
                     PGATILQVSKDLDINIDKIKRFLKDERLEIIGDEGIPVLECEKCGKPISTGRFCDTCS
                     RELAADFTSAARDIQKANNMAEYGTGRGLRYLSREKDWEKQGGFTSNKAPYQRNKKKE
                     I"
     gene            7050..7334
                     /gene="flgM"
                     /locus_tag="PK733_07250"
     CDS             7050..7334
                     /gene="flgM"
                     /locus_tag="PK733_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOJ10372.1"
                     /translation="MKIWGNIPGISGIYGKNKVNRVNETSGVSSKNDEISISEKARDY
                     QFALKHLKDIPDIRRDRVDELANKIKSGNYNVNGKEIADKIIKSAFDKKA"
     gene            7359..8015
                     /locus_tag="PK733_07255"
     CDS             7359..8015
                     /locus_tag="PK733_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOJ10373.1"
                     /translation="MINRILPDISMADKLADVPEIIDNLADVLEEEASLYSDFLKAAR
                     DKTDIIVNGKAADLEGIVKKEQAFVSQMQKLETAREQIVKDMAEKSGIDYSKLTVTSI
                     IEMMSTATRMAPAGKTQMALREAAEIALTKAIGMTSAEAAAENRVKRLKNSRDNIIDL
                     VNEIKKVNELNSKLIANSLEYINFSINLIASANSTGSKYGNDGKEGKVERRSLFDVKL
                     "
     gene            8139..9578
                     /gene="flgK"
                     /locus_tag="PK733_07260"
     CDS             8139..9578
                     /gene="flgK"
                     /locus_tag="PK733_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOJ10374.1"
                     /translation="MAFGFGSYQIAKSGLYVNEQALNITGHNITNMNTPGYVRQQVII
                     KDAPYQSSLYDRGNLQQIGLGADLQQIRQIRNDFLDDIYRNENTDLGYWEARQKSVQE
                     VEAILADPMGKGLQWAMNQFWDSWQELAKEPESLTVKAIVRQRGEALVNTINHISSQF
                     DKLRSDLGEEINTRINEVNKITSQIAELNIDIFKYEAAGGTANDYRDQRNLLADRLTK
                     LINADITENQEGLMFITIEGHAIVNRSKSVNLYVDMDKEPGKKLSYTPKIEGSEISVP
                     IKGGLIGGLIDSVKEVDDVKNLLNELIGTMTEEVNRLHKSGKTMGNPPKDGEDFFTSL
                     KIGSDGGNIGLGNIKLNDNLKDPNNIVASKSGYSGDNTIALDIANLRHAALIADNTGI
                     LNMDEYYRTIILKVGQSGNKAAEISENQRKLVQAADMNRQTLSGVSLDEEMTFMMKYK
                     FAYDASSRAFNAIDDMIDTLINRMGIVGR"
     gene            9683..11332
                     /gene="flgK"
                     /locus_tag="PK733_07265"
     CDS             9683..11332
                     /gene="flgK"
                     /locus_tag="PK733_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOJ10375.1"
                     /translation="MSGSFFGFNTAVRGLFTAQKHLDVINHNVNNVNTPGYSRQVAAQ
                     VAAKPSPLPGILGMMGTGSDITSINRIRDEYLDYKYWSENKAYGEWTAKMELLAEMEV
                     TFNEPSDSGFTKIMNEFYSALQELVKDPSSAPPRALVQQNGVTLVRYFNSTAVHFEKL
                     QADLNQQIRIKVEEINSLAEQVQKLNKQIYTVELDGSMANDLRDRRTLLVDKLSKIAD
                     INVNEVVAGKLPNGNPEKHFTITIGGKALVSHFNVSRLQVTQRDESTRLNEEDIPDLY
                     EISWKDGNKFVAKSGELRAYLDVRDGNDGQVGVDGERESPSFKGIPFYIRQMNEFVST
                     FAMAFNEGYTDENGNGIIDPGEDGIGHADGYGLDPDGGGPLEPQKGIRFFTMLGSDGK
                     PMSSEDFISGETSIEGIVDKYKHITAKNFAVSEDVLNNHNNIAASDVPGEVGNMKVLG
                     ELLNMRHNTHMFSEGAPEDFMKSLIATLGIDSQQAIMHSDNQKAMIKQVDNCRQSDSG
                     VSLDEEMADMVRFQHAYNASARMITTMTEVYDTLINRLGLR"
     gene            11443..12354
                     /gene="flgL"
                     /locus_tag="PK733_07270"
     CDS             11443..12354
                     /gene="flgL"
                     /locus_tag="PK733_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOJ10376.1"
                     /translation="MRVTNNMLINNMVGYLNNNLNRLNKYQNQYSTGKKISVPSDDPV
                     VAARALKLRTDLAEVEQYKRNADDAMSWLEITEDTVASIGEILGRARVLAGDASNGTK
                     TPSDMEKIKVEIEQLREQIIQLSNTTYAGRHLFSGFSTNSPLINKDGKFAIDVSNSNE
                     KILFEIGIGDNMNVNVPGGDLFNNGADAAAGKVPALIKLFDDYIAALGSGNHEDVNNI
                     LGEIDTAHDNLLRVRADIGARTNRLELTKDRLEHDTINFTKLMSLNEDVDMAEVIINL
                     KNEENVYRASLGAGARIIMPTLMDFLR"
     gene            12545..13093
                     /locus_tag="PK733_07275"
     CDS             12545..13093
                     /locus_tag="PK733_07275"
                     /inference="COORDINATES: protein motif:HMM:NF039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HOJ10377.1"
                     /translation="MIGRIGIAGSGASYSPGKRANIAASNALDSGSSGHIAVLRKDNT
                     ILPKINTAHDAHGKSNNLLKKMNIITRSREAILEIDQYPVFAGLGYKSSGDLVKESTQ
                     RGYRYSMQYIGKTAADGDRLAAIELGGEPLADIAERDAFPENDSGIGTVPGAGPEFDV
                     KYITRMYKTDGTLVGTKVDRFI"
     gene            13150..13689
                     /gene="fliW"
                     /locus_tag="PK733_07280"
     CDS             13150..13689
                     /gene="fliW"
                     /locus_tag="PK733_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOJ10378.1"
                     /translation="MLFNTRHFGEIEINEDKIISFEEGIPGFNNVRKFVILDTYTTND
                     NRYDNMNNQNNSVSPFKWLQCVDEPMLAFAIVNPFLLKPDYDIELSNEVIEQLGIKNE
                     EDVAVYAIVVVPEDISKMTMNLKAPLIINLKSKKGMQTILDTEKYSVRHYIMDEKAGQ
                     EVKANACADKKKGSVNCNR"
     gene            13643..13885
                     /gene="csrA"
                     /locus_tag="PK733_07285"
     CDS             13643..13885
                     /gene="csrA"
                     /locus_tag="PK733_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOJ10379.1"
                     /translation="MLVLTRKKDQSIVIGDNIEISILEIVGDQVRIGIDAPKNVAIHR
                     KEIYLEIQEENRKAAESNTISQAALNEILKGKNKDS"
     gene            14104..15597
                     /locus_tag="PK733_07290"
     CDS             14104..15597
                     /locus_tag="PK733_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOJ10380.1"
                     /translation="MRINNNIMAMNTYRQLGLANAAGARSMGKLSSGFRINNAADDAA
                     GLAISEKMRGQIRGLTQASRNAQDGISFIQTAEGALGETHAILQRMRELAVQSSTDTN
                     VAVDRSEIQKEMDELAKEITRIANNTEFNTQKLINGGITSTGLGNVKFHVGANASQDI
                     GLSIKAMDAKSLGVARDVLEANVTSATKVDSVVIDDLNKGTAIINGATLSLTADATGA
                     KTEWTAVALGITGTSLASANNSSQYNDYTITVTKTGVVGADVTAAIDTNAKTITINYA
                     SDVAGAASTATKLQAALDAQSIMLTATGDATAVAGVTNAAATTAGLGNDEVKLTLGTE
                     NLIVKNNVSSATFASSANKGVTVNFDGTTLDDTVAATIGITSNASTAATFANGEVDKE
                     ANAAAGIDVSSQTTASAAITTIQRAIETVSAERSKLGAYQNRLEHTIKNLDTSAENLQ
                     ASESRIRDVDIAKEMMELTKNNILQQAATAMLAQANQLPSGVLQLLR"
     gene            complement(15787..16152)
                     /locus_tag="PK733_07295"
     CDS             complement(15787..16152)
                     /locus_tag="PK733_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10381.1"
                     /translation="MSEKPLKENRLLQKENKIYATEGYALIEKRVRGKIPLPLFVISL
                     LFLENSIILITEVLLEVLGLLADHHIPFNMCQTISSTIYLRINYHLQYMKNGCRVLRG
                     HLCPYGSHCHGIVTLGSTS"
     gene            complement(16228..16509)
                     /locus_tag="PK733_07300"
     CDS             complement(16228..16509)
                     /locus_tag="PK733_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10382.1"
                     /translation="MEIHFIEHPESRRKEIEFNETLEKPGSINEIRVEYEYREKAMRD
                     ELSRIQGAREEGERKKAELTARNLLNINISVEITSKATGLSKEEINKLK"
     gene            16709..17806
                     /locus_tag="PK733_07305"
     CDS             16709..17806
                     /locus_tag="PK733_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HOJ10383.1"
                     /translation="MNKILKDNMLDVVEHQIKLNKPECAKEEYKRLVGLGISKCRAKE
                     MMAEVLLEEICHNMENNERFNTKRYCRKLRSLESDLPPEDNIYRYLTIQEILNSIENN
                     YGDFPDDALLEIIRRKEEAVPYLTEILKKVQENPKEYAENEDYFGHIYAAYLLAQFRA
                     TEAYPAFIQLLKIPKEFSYELLKDTVCESGGRILASIYDGDINPIKNLITDTGLDEYI
                     RGAGVKALTALVLQDALPREEVIEYFRILLTGGLTDENTLVIGDIVSSCDSLYPEDLM
                     GEIKAVHQKGLIGNKFVNFDNLEETIAYGKDWILEQNKYDLYYEYINNTIDELYWWHC
                     FNEDSSFADIIIEDMYIHAEEAIIKSKIKQE"
     gene            17970..>19427
                     /locus_tag="PK733_07310"
     CDS             17970..>19427
                     /locus_tag="PK733_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10384.1"
                     /translation="MKNLENVKEILMHKKSTTMAQDIFMNDLNDIYFELDKKERDFLE
                     KSIDQIFRKYYYSAVEPETYITPANILSSLAEKESAGNTVFVPKVELIKKKDVLTKIK
                     SSLHYVNSENHYMVTDFETVLEICKESLKVNIIGAPEDSERKRFEDRLFLNDRHYLNI
                     IVLVAIKLGYIQLVKQGKKNMAITTEKSKELLGMSMKDKIKIPAKGIVENYVSSSVLE
                     IPELKDIITNNAIWEIIKNPIKFEDFMYSVNEKVGLDTDIFNKIIHDKELEYEQLEDM
                     DANNPYSYMLAMFLIHIDVYLFTPLGYYLQLIKPFYGNVYNIAIEVGEIIAVLSGNYE
                     HVRNMLFFVPEKFDLTLFGEEILLDGGKSERRQEIPEKYSDNSFLKCFEIREQFINEE
                     LLNLIDNYGGFDEDDDDDEDYDDNSLDEDYDDEDDDDEDYNDEDYDNDDYEDDDYDRD
                     YEKEGFTKSEHKYDNIIDLSNYKKEDSSIERNYRKR"
BASE COUNT         6877 a         2975 c         4294 g         5281 t
ORIGIN      
        1 caggggcaag agtatgcatg gaaattttct gcgctttcct ctgtcaccgg gctggttctc
       61 ggaataatac ttgctattac tgcaaaattt actctatcat tctttaatgt atctgtcgag
      121 gtatataata gcacattgtg gatgcaatat atgatggccg tcataatggt atttagagtg
      181 tttacgattg ttgttgctgt tggtatttta agagggggcg gggatgcaca ttttgcgttc
      241 atttccgaaa gtattacaat gtggttaatc ggtgttcctt tagcttttat aggagcattt
      301 gtatttcatc ttccaataca ttttgttgtt gccctttcta caatagaaga gctattaaaa
      361 gtttatatat tcattcgtcg cttaatttca aagaaatgga ttaataattt ggtgcgtgat
      421 gtagctttag acgtagcata gctaatttaa aagtaggtat ttataaaaca aattatgggt
      481 aaattcatat gttatttgtt caaagcgata aatgatccgt attagccaag tggaggaggg
      541 aaaaggaaat atgtccctac tagacttaat atttcttccg ttgttgctga agtgaatatt
      601 atacatggaa atcgtttctt gctatatttt ccctcaaata gaaaatgcgc cctctggaaa
      661 attccaactt gacttaatta tacgaatatg ctaaagtatt ttagacaaac taaatacagt
      721 tttaccttat caagagaggt ggagggaatg ggcccgatga aacccggcaa ccagcatata
      781 atatgcaatg gtgccaattc ctacaggata tgtgttttat atatcctggg agatgaggag
      841 cgatatcaaa acctcttcat tcaagaggct tttttatttg ttccggagaa acaatcttga
      901 gtagaggaga aagagtcaaa tgaaagggaa aaaagaagaa atggaaagaa aactgtttac
      961 atcggaatcg gttacagaag gccatccgga caaaatttgc gatcagattt ctgacgcagt
     1021 acttgatgct atttatgagt gtgatcctat agcaagagtt gcctgcgaaa cagcggtcac
     1081 aacaggatta gttgttgtta tgggtgagat cacaacccac tgttatgtgg acataccgaa
     1141 gatagttaga gaaactatca gggaaatcgg ttatgacagg gctaaatacg gatttgactg
     1201 tgatacatgc gctgttatta catcaataga tgagcagtcc ccggacattg ctttaggtgt
     1261 aaaccaggct ctggagtcaa aaaatggcga aatgaatgat gttgaagtag aggctatagg
     1321 tgcaggggat cagggcatga tgttcggctt tgcctgtgat gaaacgcctg aattgatgcc
     1381 aatgcctata tctcttgctc acaagcttac cagaagattg agccaggtaa gaaaagatgg
     1441 aaccataccc tatttgaggc ctgatgggaa atcacaggtt actatagagt atgaagggca
     1501 tactcctgtg agagttgata ctgttgttat atccagccaa catagtcagg aagtggaaca
     1561 tgatacaatc gagaaagaca taattgagca tgttataaaa cctatacttc ctgttgaatt
     1621 aattgatgaa aaaacaagga ttctggttaa tcctacagga aagtttacag ttggaggacc
     1681 tcagggtgac tcaggcttaa caggaagaaa gattattgta gatacttatg gtggatatgc
     1741 ccgccatgga ggtggagctt tctcagggaa agatcctaca aaagtggaca ggtctgcttc
     1801 gtatgcagcc cgttatgtag ctaagaacat tgtggcagca gggctggcaa gcaagtgcga
     1861 agtacagctt gcatatgcca taggagttgc aaaacccata tcaatttccg ttgacacata
     1921 tggcacaggg actattcccg attcagaaat agtaaagctc attaacagtc actttgattt
     1981 aaggcctgct gcaattatta aaatgctgga tttaagacgg cctatatata aacagactgc
     2041 tgcttatgga cattttggaa ggacagatat tgacctgccc tgggaaaaaa ttgataaagc
     2101 tgaaattttg aaaaaggatg cagggattta agaaagccaa tataaatacc agccaataca
     2161 acggttggca gtattcgata aacggggtgt gacatggttc atatcccgtt tattttaaac
     2221 tatacaaatt atccagacac ataacctgga caaatcatct ggacaaatta tctggacaaa
     2281 ttatccgggc gtatcaccaa acaaaaggtt aagtcagtca atattgggta ttttctaaac
     2341 atgcacaaaa ctgtgaacac ttttcatatt atgaagaagg atatatttta aaggggtgtc
     2401 tgctattgtt tatacaaata tttaatattg ctatgtacat attggcatta tatggtataa
     2461 tatcattaat aatgtcgtat gttcgtcatt tgtggcgtgg ctcaggtata aaacacttaa
     2521 ataccaatat gagaatggta ctgattgcaa aagatcagga ggatgttatt gaaggtataa
     2581 taagaagcct ttatattagc ggcgtttcag aaggtggtgc aggtatttat gtagttgatg
     2641 cggattcaca ggataaaacc ctggagatac tgcaaaagct gaaccgtaca tatgacaata
     2701 tgaggatatt ggcttttaat gaaagagata ggatatttga tgggttcatt gaagatattg
     2761 aggctttgga agatactgaa gtagatattg gagaagatat tgaagacatt caccatattg
     2821 aagaccttga aaatattaga caaatgtgaa tactgaaaat ataatataaa aaacattaac
     2881 ataacaaaag gtgaagatat tgattattgt tgaaggaaca attgaaggta taatttttac
     2941 taatgaatca aatggatatg cagtgtgcga aatagcccac gagaagggtg ttgtaacatt
     3001 agtgggctac atgcctttta taagcgaggg agagattatt aaggccagtg gcttatgggt
     3061 tactcatccc gattatggtg agcagtttaa ggtggaatac ttcgaaaaaa ttttgccaaa
     3121 tactgcagag gccatagaaa agtatctggg taccggtata attaagggta ttgggaaagc
     3181 aactgcacga aaaattgtca aaaagtttgg agacaatgct ctaaatgtta ttttggagaa
     3241 tccggaaaaa cttcgcgata taaagggaat gagccgtgca aaggctcttg aaattggaca
     3301 ggctttcgcc aaacgcaaaa actttacaga tatagtctct ttccttcagg agtatgggat
     3361 aagtcctgct tatgcagcaa aagtttataa gatattcgga gaaaatacta ttgaagaaat
     3421 acgggcaaac ccctacaggc ttgctgatga aatacccgga atcagcttta aaacaaccga
     3481 ccgtattgca aatagccttg gtatagaccc tgaatccaga cttagaatat gcagcgggct
     3541 taaatatgtt ctcattagag ctgctgtaaa cggacataca tatctgccat atgatgagct
     3601 tgctaaatac ggtgaaaatc ttctaggtgt tagtgcaggg catatatccg atgctcttgt
     3661 gtttatgcgt tttgatggaa caatctatat tgaaaaagaa aatttgagaa ataattttaa
     3721 tgataatttt aataataatt ataatgataa atttaatgac agtttgaaaa gcaatataag
     3781 gtacaatgca aaaaacaata caatagatga ttatgctggc gaacatgaga acgatgcaga
     3841 aagttctgat ggcagagtat atctgagtga tttgcatcag gctgaactca atattaccag
     3901 aagactcaca gctttggcaa actcagtact tctcctaaag atacgaggta ttgacaaaac
     3961 gctggagaaa actctgaaag atttgcagca ggaagaaaat atcatactgg ctgagacgca
     4021 aaagactgca ataaaagaag cactgggtaa tggagccttg gtaattacag gaggaccggg
     4081 tacaggtaaa actactataa ttaaaagtat tattaaaatt tttgagagga acaaatttaa
     4141 agtagcactg gctgccccaa cgggtagagc tgcaaaacga atgacagagg ctacgggtta
     4201 tgaatccaaa acaatacacc ggctgctgga aattggctat gtaggtgaag ataatgagat
     4261 atctttcatg aggactgatg taaatcctat tgatgccgat gtcattatta tagatgaaat
     4321 gtccatggtg gatattctgc ttatggatag tcttatgaag gccattaaac ctggctcaag
     4381 gctgattctt gtgggggatg tagatcagct tccttcagta ggcccgggaa atgtactgaa
     4441 ggatataata tcaagcggag caatcaggac agtcagactt accgaagtat tcagacaggc
     4501 ggaaaaaagc ctgattgttg ttaatgccca ccgcatcaac aggggagaaa gacccatatt
     4561 aaatgaaaaa agcaaagact tctttttcat ccctcgtaat aaccccaccg atattgttaa
     4621 cttggtgctg gacctgtgtg gcaggagaat tccttcacaa ttgggatatg acccgttgcg
     4681 ggacattcag gtattgacac ctacaaaaaa gggcctggta ggcgttggaa atcttaatat
     4741 agaactgcag aaagagctga atcccaaaaa taagaaccgg aatgaaaaag cattcccgaa
     4801 tttcaccttc cgtgaaggcg ataaggttat gcagataaaa aacaattata acatacaatg
     4861 ggaaaagata caggaacaga aggatggttc tcggcaggaa caaagagaag gttctcggca
     4921 ggaatatggg gacggttcca gtgtttcaca tggtaattat gcatccggta attatttatt
     4981 tgatgataat gaaaatgatg ataacgtgct tggtgatggc ggcacagggt ataaccaact
     5041 agggaacagc caatttgaga ataatgagac tgggaataac cagacagaga ataatcaggc
     5101 tgagaataac ggaggagaag gcatttttaa tggcgatacc ggtgtaattc gtcagattga
     5161 tcatgacgac gaaaaaataa gagtggtttt tgatgataac cggcttgttg agtactattt
     5221 cgatgctttt gatgaaatag aacctgcata tgcaataact atacataaaa gccagggcag
     5281 tgaattccct gtggttattc tgcctatctt tccggggcct ccggttctga tgacccggaa
     5341 cctgttatat actgcaataa ccagagcaag agatttagtc atagttgtag gactcgaagg
     5401 tactctttat cagatgattg ataataacag ggagacattg agatattcgg gcctggcgga
     5461 caagctgcaa aaaagtgccg gatctggcgg atattaagca aattcaatgc tttgcttttg
     5521 ctatgatttt aagagcggtg atttaaaccg accctggagg tgatttgact gactctgaag
     5581 gagattgccg cattagctgc cagactgctt tttcccccaa agtgtatctt ctgttcatcc
     5641 atacttgacc tgaaagcaga aatagaagta tgcaaatcat gccgcaaacg gttaacatat
     5701 attggaaaca aattatatta tataaacaaa ttttcatata agaacatgac accttgcagg
     5761 tattttgaca gggttttttg tgtatttgaa tactccggct atgtaagaga agctattgta
     5821 aggtataagt attttgggaa atcccattat tataagactt ttgctgctct attggctgat
     5881 aaactaaaaa gaattacatg cagtggagat tttaatgtaa ttttaagtgt accacttcat
     5941 aaacataaag aaagaaaaag aggatataat caatcacgtt tgatatcaaa ggaattgagc
     6001 aggataatgc aggtgcccga aagatcggag cttctggaaa gaataaagaa tacccctgct
     6061 cagagcctac ttcataaaaa tgaaagatat ttaaatataa agaatgcatt taaagttacc
     6121 gatgtattag aagtaaaggg aaaatcaatt ttgcttattg atgatgtact gacaacggga
     6181 aatactctta acgagtgcag cagggtactt aaagaagctg gagcaaaaaa ggttgatata
     6241 gcagtaatag cagttgcgta tacatttgag ttgtagtgtt atataagata aagtgatata
     6301 gcccctgggg gcagaaaggt tacctatttt caaataagca aaaagataag gagatatagg
     6361 gatattatgg cagatttaag gaattgtaat agatgcggga aattgcataa tttcataggt
     6421 ggatcactgt tatgcccctc atgcagggat gaagatgaag cagattttca aaaaataaaa
     6481 agatatcttt atgaaaaccc aggtgctaca attttacagg tttccaaaga tctcgatatt
     6541 aatattgaca aaataaaaag gtttctgaaa gatgaaaggt tagaaataat aggagatgaa
     6601 ggcatacctg ttctggagtg cgaaaaatgc ggtaaaccaa taagtacagg gcgcttctgc
     6661 gacacctgca gccgtgaact tgccgcagac tttacatcag ctgcccgtga tatacaaaag
     6721 gcaaacaata tggccgaata tggcacggga cgaggactga ggtatttatc aagagaaaag
     6781 gattgggaga aacaaggcgg ctttacaagt aataaagcac catatcaaag aaataaaaaa
     6841 aaggagatat aaagtaaaca aagcagcgtt ataagtcata aaaccattaa ggaagaaaat
     6901 gagtttaaaa aagtctacta taaaacattt actagctggg aatactctga cttttttatt
     6961 aagaaaattt tttatttacc gataatatta ttgtaaatga tgaaaattgt aaaggataaa
     7021 aaattaatgg aaaaagaggt gaaattgtta tgaaaatatg gggaaatatc ccaggcattt
     7081 caggcatata tggaaagaat aaggtaaaca gggtaaatga aacatcaggg gtttcatcaa
     7141 aaaatgatga aatatcaatt tctgaaaagg caagagatta tcagtttgcg ctaaagcatc
     7201 taaaagatat acctgacatt cgcagggata gggttgatga acttgctaat aaaattaaat
     7261 caggaaatta taacgtaaat gggaaagaaa tagcagacaa aataattaaa tcagcctttg
     7321 ataaaaaggc ctgaagactg cctaacgggg gactaattat gattaatagg atattaccgg
     7381 atatttctat ggcagataaa ttggcggatg tgcctgaaat aatagataat ttagcggatg
     7441 tactggaaga agaagcatcc ctttatagtg attttctaaa agccgccaga gacaaaacgg
     7501 atattattgt gaatggaaaa gcagctgatt tggagggaat tgtaaaaaaa gagcaagctt
     7561 ttgtatcaca aatgcaaaag cttgaaactg caagagagca aattgtaaag gatatggctg
     7621 aaaaatccgg tatagattat tcaaagctaa cagtaacatc aattattgaa atgatgagta
     7681 cggcaactag aatggcacca gcagggaaaa cccaaatggc actaagagaa gcagccgaaa
     7741 tagcattaac aaaggcaatc ggaatgacgt cagcagaggc agccgctgaa aatagggtca
     7801 aaaggcttaa aaacagtaga gataatatta tagatttagt taatgagatt aaaaaggtta
     7861 atgagttaaa ttcaaaactc atagcaaatt ccctggaata tattaatttt tcaattaacc
     7921 ttatagcatc tgcaaacagc accggaagca aatatggaaa tgatggaaaa gaaggaaaag
     7981 tagaaagacg tagtttattt gatgttaagc tataacaaag caaattaata ctactatgcc
     8041 aatttggaaa gccatgtctt ccgggatgtt ttttgatagc atcaggcaac ataacaaagt
     8101 agtattaact ttgactattt taaggaaggc ggaactatat ggcttttggt tttggaagtt
     8161 atcaaatagc caaatctggc ttatatgtaa atgagcaggc tctcaatata acgggacata
     8221 acattacaaa tatgaataca cctggttatg taaggcagca ggttataata aaagatgctc
     8281 cctaccagtc gtcattatat gacagaggga atttgcagca aattgggctg ggggcagatt
     8341 tacagcagat aaggcaaata agaaatgatt ttcttgatga tatatacaga aatgaaaata
     8401 cggatttagg ttattgggaa gcaagacaaa aatctgttca ggaggttgaa gcaatcctgg
     8461 ctgatcctat gggaaaaggg cttcaatggg ccatgaacca gttctgggat tcgtggcagg
     8521 aactggcaaa ggaacctgaa agtctgacag taaaagcaat tgtaagacag cggggggaag
     8581 ctctggtaaa cacaattaat cacataagtt cccagtttga taagcttcga agtgatttag
     8641 gtgaagagat aaatacgcgt ataaatgaag ttaacaaaat tacgtcacaa attgcagaac
     8701 taaacattga tatattcaaa tatgaagcag caggtggtac tgccaatgat tacagagatc
     8761 agagaaacct gcttgcagat agattaacaa agcttataaa tgcagatata actgaaaacc
     8821 aggaaggcct tatgtttata acaattgaag gccatgccat tgtaaatcgc tcaaagagcg
     8881 tgaaccttta tgtggatatg gacaaagaac caggaaaaaa gctgtcttat actcctaaaa
     8941 ttgaagggtc ggaaattagt gttccaatta aaggcggatt gattggcgga cttatagact
     9001 cagttaagga agtagacgat gttaaaaatt tgcttaatga gcttataggc acaatgactg
     9061 aagaagtaaa cagacttcat aaaagtggca aaaccatggg aaatccgcct aaagatgggg
     9121 aggacttctt tacgtcactt aaaatcggca gtgatggagg caatattgga ttaggcaata
     9181 ttaagttaaa tgacaactta aaagatccaa ataatattgt agcttccaaa tctggatatt
     9241 ccggtgacaa tactatagca cttgatattg caaatctaag gcatgcagcc ttgatagcgg
     9301 acaataccgg gattttgaat atggacgagt attaccggac aataattctt aaagttgggc
     9361 aaagcgggaa taaagcagca gaaatctctg aaaatcagag aaaacttgtc caagcggcag
     9421 atatgaacag acaaacactt tccggagtat ctcttgatga ggaaatgacg tttatgatga
     9481 agtataaatt tgcatatgac gcttcttcta gagcctttaa cgctatagat gatatgattg
     9541 atactctgat aaaccgtatg ggtatagttg gaaggtagac aagcaatgta atggaaggca
     9601 gtaaaacaat aaaagctagg tatgtaagcg gtacataata agtttatata gtaatagtat
     9661 aagagtagag tggtgactaa atatgagcgg gtcttttttt ggatttaata ctgcggtaag
     9721 agggcttttt actgctcaga aacatcttga tgtaattaat cataatgtaa ataatgttaa
     9781 tactcccggg tattcccgtc aggttgcagc acaggtagca gcaaagcctt ctcccctccc
     9841 gggtatttta ggaatgatgg gtacagggtc tgacataact tcaataaata gaattagaga
     9901 tgaatacctg gattataaat actggagtga aaacaaggct tatggcgaat ggacggccaa
     9961 aatggaactg ctggcggaaa tggaagtgac ttttaatgag ccttctgaca gtggtttcac
    10021 taaaataatg aatgagttct acagtgctct ccaggaactg gtaaaggatc cttccagcgc
    10081 acctcccagg gctcttgtac agcagaatgg ggtaaccctg gtcaggtatt ttaacagcac
    10141 tgccgttcac tttgagaagc tgcaggcaga tttgaatcag cagataagaa taaaggttga
    10201 ggaaataaac tcacttgcag aacaggttca aaaactgaac aaacaaattt atactgtaga
    10261 gctggatggc agtatggcaa atgatttaag agacagaagg accctgttgg tggacaagct
    10321 ttctaaaatt gcagatatta acgtaaatga agttgttgca gggaaacttc ctaatggaaa
    10381 tcctgaaaag catttcacta taactatagg cggtaaagcg cttgttagcc atttcaatgt
    10441 aagcaggctt caggttactc aaagagatga aagtacaaga ttaaacgaag aggatatacc
    10501 tgatttatat gagataagct ggaaagatgg gaacaagttt gttgctaaaa gcggagaact
    10561 cagggcttac ctcgatgtgc gggatggtaa tgatggacag gttggagttg acggagaaag
    10621 ggagagccct tctttcaaag gaataccctt ttatataaga cagatgaatg aatttgtcag
    10681 tacatttgct atggccttta atgaaggcta tactgatgaa aatgggaatg gaataattga
    10741 tcctggtgaa gatggcatag gccatgcaga tggatatggt cttgaccctg acggtggagg
    10801 tcctcttgag ccgcagaaag gtataaggtt cttcaccatg ttaggcagtg acggcaagcc
    10861 catgagcagt gaagacttta taagtggtga aacatccata gagggaatag ttgataaata
    10921 taaacatatt actgccaaaa actttgcggt aagtgaggat gtattgaata atcacaacaa
    10981 cattgcggca tccgatgtgc cgggtgaggt aggaaacatg aaggttctgg gagaacttct
    11041 taacatgaga cacaacacac atatgttttc tgaaggtgca cctgaagact ttatgaaatc
    11101 tcttatagct acccttggta tagattccca gcaagctatc atgcattccg ataaccagaa
    11161 ggctatgata aagcaggtag ataactgcag acagtctgat tcaggggttt ctctggatga
    11221 agaaatggca gacatggtaa gattccagca tgcatacaat gcatctgcac gtatgattac
    11281 gactatgacc gaggtttatg atacactgat aaacaggctg gggttgaggt agacccctag
    11341 aggtatatgt gcacaaaaat ttcatatgta ttttcttttg tgcgttatct atagcttatg
    11401 ttattgactt gatagattaa agatattttg gaggttcgga caatgagagt aacgaataat
    11461 atgctcataa acaatatggt aggttatttg aataacaact tgaacagatt aaacaaatat
    11521 caaaatcagt actctaccgg gaaaaagata tcagttccct cagatgaccc tgtagtagca
    11581 gcaagagcct tgaagctgag gacggatctg gctgaagttg agcagtataa gagaaatgcc
    11641 gatgatgcta tgtcctggct tgaaattaca gaggatactg tagcatcaat cggtgaaatc
    11701 ttaggcagag caagggtact tgcaggagat gcaagcaacg gaacaaaaac tccatcagat
    11761 atggaaaaga ttaaagttga gatagagcag ctgagagaac agataattca gctttcaaac
    11821 acaacttatg caggaagaca cttgttctca ggctttagta ccaactctcc gcttataaat
    11881 aaagatggaa aatttgctat tgatgtatca aacagcaacg agaagatatt gttcgaaata
    11941 ggcataggag acaatatgaa tgtaaatgtg cccggaggag acttgtttaa taatggagct
    12001 gatgcagcag cagggaaagt accagctttg ataaagctgt ttgacgatta tattgctgcc
    12061 ctgggttctg gaaaccacga agatgtaaat aatatattag gtgaaatcga tactgctcat
    12121 gataacctcc tgagagtaag ggctgatata ggagccagaa caaacagact ggaactgact
    12181 aaagatcgtc tggagcatga tacaataaac tttacaaagt tgatgagttt aaatgaagat
    12241 gttgacatgg cagaagttat aatcaattta aagaacgaag aaaatgtata cagggcatct
    12301 ctgggagcag gagcaaggat aataatgccg acgttgatgg actttttaag gtagcataag
    12361 tcaggggtgc atttacaaaa tttgaagaaa tatttaccag ttgctatcat tacaatgctt
    12421 agaacctcca tgtatgtctt ttaacaatat tttcttcaga taaattaaat gcacccgtaa
    12481 gtcagaaaga cagtaattta gtgagttagt aagttagaac attatggagt tagggagtca
    12541 cggcatgata ggcagaatag ggatagcagg aagcggagca agctatagtc cagggaaaag
    12601 ggcaaatatt gcagcaagca acgcactgga ttcaggttcc agtggacata ttgctgtact
    12661 gagaaaggat aatactatat tgcccaaaat taatactgca catgatgcac atggaaaaag
    12721 taataacctg ctaaaaaaga tgaatattat tacccgcagc agggaggcaa tattggagat
    12781 agaccagtat cctgttttcg caggtttagg ctataaaagc tccggtgatt tggtaaaaga
    12841 atctacacag agaggctatc ggtattctat gcagtacatc ggcaagacag cagccgatgg
    12901 ggacaggctt gcagctatag aacttggtgg tgaaccgctg gcggatatag ctgaaagaga
    12961 tgccttcccg gaaaatgatt ccggtatagg gactgtgcct ggagcgggac cagaatttga
    13021 tgtaaaatat ataacaagga tgtacaagac agatggaacc ttggttggga caaaagtaga
    13081 caggtttata taaggatagg tttatataaa ataaagcagg aaaacctagt aaaaaggggg
    13141 aagagagaca tgctttttaa tacaaggcat tttggagaaa tagaaataaa cgaagataag
    13201 ataatttcat ttgaggaagg aattccgggg ttcaataatg taaggaagtt tgtaatactg
    13261 gatacatata ctactaacga caatagatat gataacatga ataatcagaa taattcagtt
    13321 tcacctttca aatggctcca atgtgttgat gaacccatgc tggcctttgc aatagttaat
    13381 ccctttttat tgaagccgga ttatgatata gaattaagca atgaagtaat tgaacaactt
    13441 ggaataaaaa atgaagagga tgtagctgta tatgctattg tagtagtgcc ggaggacata
    13501 tccaaaatga ctatgaacct caaggccccg ttaattataa atctgaaaag caaaaagggc
    13561 atgcaaacta ttcttgatac tgaaaagtac agtgtaaggc attatatcat ggatgaaaaa
    13621 gccggacagg aggtaaaggc aaatgcttgt gctgacaaga aaaaaggatc agtcaattgt
    13681 aataggtgat aacatagaaa tatccatact tgaaatagtg ggagatcagg tgcgtatagg
    13741 aattgatgca cccaaaaatg tggctataca tcggaaagag atataccttg aaatacagga
    13801 agaaaacagg aaagcagcag aaagcaatac catatctcaa gctgcgctaa atgaaatatt
    13861 aaaggggaaa aacaaggata gctagggtgg ctatggaaaa aattatgaat atttttctaa
    13921 ttaccctaaa gtttctataa gaacctaacg atatataaag taagaagtaa taaagtgaat
    13981 tttcatagcg gccgtatgaa aagcacttta tgtaataaac aaaataaaat atttgaatca
    14041 caaaaacatt tcaaaaatac cgggcaagga cgcctggtag taaaaaaaca gggaggtaat
    14101 attatgagaa ttaataacaa cataatggca atgaacacct ataggcagtt gggacttgca
    14161 aatgctgccg gggcaaggtc aatgggtaag ttatcatcag gctttagaat caataatgca
    14221 gctgatgatg cagcaggtct tgcaatcagc gaaaaaatga gaggacaaat cagaggatta
    14281 actcaggctt ccagaaacgc tcaagatggc atttctttta tccaaacagc agaaggcgca
    14341 ttaggagaaa cacatgcaat tcttcaaaga atgagggaac ttgcagtcca atcatctact
    14401 gatacaaatg tggcagttga tcgtagtgag atccagaagg aaatggatga attagcaaag
    14461 gaaataacca gaattgctaa taatacagaa tttaatactc aaaaacttat caatggtgga
    14521 attacttcaa ctggtctagg taatgtaaaa tttcatgtag gtgctaatgc atctcaagat
    14581 attggattat caataaaagc catggatgca aaatcattag gagttgctag ggatgtcctg
    14641 gaagcaaatg ttacatcagc aacaaaagtt gatagtgttg tcattgacga tttaaataaa
    14701 ggaacagcta ttataaacgg agctacattg tctttaactg cagatgctac tggggctaaa
    14761 acagaatgga cagcagttgc attgggaata accggaactt cattagcttc tgcaaataac
    14821 tcatctcagt ataatgacta tacaataact gttactaaga caggagttgt aggtgctgat
    14881 gttacagctg caatagatac taatgcaaaa acaattacta ttaattatgc aagcgatgta
    14941 gctggggctg catctactgc tacaaaacta caagctgcac tagatgcaca aagtataatg
    15001 ttaacagcta ctggtgatgc tactgcagtt gcaggagtaa ctaacgctgc tgcgactact
    15061 gcaggtttag gtaatgatga agttaaatta acactgggaa cggaaaatct gattgtaaag
    15121 aataatgttt catcggcaac atttgcaagt tcagcaaata agggtgtaac agttaatttt
    15181 gatgggacca cattggatga tacagtagct gcaaccattg gaataacatc caatgcatca
    15241 acagctgcaa cttttgcaaa tggtgaagtt gataaagaag caaatgcagc agcaggaatt
    15301 gatgtttctt cacaaactac tgcaagtgcg gctatcacaa caatacaaag agccatagaa
    15361 acagtttctg ctgaaagatc aaaacttggt gcgtatcaaa atagattaga gcacacaatc
    15421 aagaatttgg acacatcagc tgaaaacctg caagcttcag aaagcagaat cagagacgta
    15481 gatatagcga aagaaatgat ggagctcaca aagaacaaca tcctgcaaca agctgcaaca
    15541 gctatgctgg ctcaagcaaa tcaactaccg agcggagtac tgcaactcct tagatagttg
    15601 aaataagggt ccattagaga gtaatctcta atagcaaatc gggtgaattg ctggaacctc
    15661 ctaaagcttt cttccctaca acgcaacctg aaaaggtcaa cgtgaaaggt taaaaagagg
    15721 aaagatgcaa caatggataa tcagcagccg agctcctgta ccgaaaggtt ggagaaggtt
    15781 caacgactag gatgtactgc ctaaagtaac tattccatgg caatgagatc cataggggca
    15841 gaggtgccct cgaagtaccc gacaaccatt tttcatatac tgtagatgat aatttattct
    15901 caggtaaatg gttgaagata tagtctggca catattgaaa ggtatgtgat ggtccgcaag
    15961 caaaccaagc acctcaagga gtacttcagt tattaagata attgaattct ctaaaaatag
    16021 taaagaaata acaaagagag gcagggggat tttccctctg actctctttt caattaaagc
    16081 atacccttct gtcgcatata tcttattttc tttttgcagc aacctgtttt ctttaagggg
    16141 cttttctgac atcaagcctg gaacctgata ccgggcttga gtcagcagac aaagaagttc
    16201 ttcacagaag acctttatag aaactcttta cttaagcttg tttatttcct cttttgatag
    16261 gcctgttgct tttgatgtta tttcaacact aatattaatg ttgagtaagt ttcttgcagt
    16321 aagctcagcc tttttcctct cgccttcttc ccgagctccc tgtattctgg atagctcatc
    16381 cctcattgcc ttttctctgt attcatattc tactcgtatc tcgtttatgc ttccaggctt
    16441 ttccaaagtt tcattaaatt caatttcctt ccttctggat tcaggatgtt caataaaatg
    16501 tatttccata atatcttttt catatgctaa ctatattatc tatagtgtgt cctagtaaaa
    16561 tcatatgtta aatttcagga ttatatggtg ggatatgtta acgatgcttt tgctttttac
    16621 atttacttct tttatttcca ttatatacgt ggtataataa aaagtaacaa tatggtaatg
    16681 atggaaaata ataaaggaga gtgctaatat gaataagatt ttgaaagaca atatgcttga
    16741 tgtggtggaa caccaaatca agctgaataa acctgagtgc gcaaaagaag aatataaaag
    16801 actagtgggt cttggtatca gcaaatgcag agcaaaggaa atgatggcgg aagtcttgct
    16861 ggaggaaata tgccacaaca tggaaaacaa tgagcgcttt aatacaaaac gatactgcag
    16921 aaaattaaga agcctggaaa gtgatttgcc tccagaagat aatatttaca gatatcttac
    16981 aattcaagag attctaaatt ctatcgaaaa taactacgga gattttcctg atgatgcctt
    17041 acttgaaata atcagaagaa aagaagaagc tgtgccatat ctcacggaaa ttttgaagaa
    17101 agtgcaggaa aatcccaagg aatatgcaga aaatgaggat tacttcggac atatctacgc
    17161 agcttatctt ttagctcaat tccgggctac agaagcttat cctgctttta ttcaactatt
    17221 gaagattcca aaggaattta gttatgagct attaaaagat actgtctgtg aatcaggcgg
    17281 ccggattttg gcttctattt atgatggtga tattaatcca ataaagaatt tgattactga
    17341 taccgggctt gatgagtaca taagaggggc tggtgttaag gctttgactg cattggtact
    17401 acaggatgcc ctgccaagag aagaagtaat tgagtatttc aggatacttt tgaccggagg
    17461 actcacagat gaaaatactc tggtcatagg ggacatagta tcttcctgtg atagtctgta
    17521 tcctgaagac ctgatgggag agataaaggc tgtgcatcaa aaggggttaa taggtaataa
    17581 atttgtaaac tttgataatt tagaagaaac aatagcgtat ggaaaggatt ggatactgga
    17641 acaaaacaag tatgacttgt attatgagta cattaataat actattgatg agctctactg
    17701 gtggcactgt tttaatgaag attcctcctt tgctgacatt attatagaag atatgtatat
    17761 acatgctgag gaggcaataa ttaaatctaa aataaagcag gaatgatcta tttaccgaca
    17821 gaagcgggaa gaagtatacg aagtgctgtg aaaagtaatt ttctgcattt ctataaaaaa
    17881 ttaaattatc acttttaaaa gcagagaaaa atagttaaaa tactaataat atacttataa
    17941 aatatatttt atatttaagg agaataataa tgaaaaatct tgagaacgta aaagaaatac
    18001 taatgcacaa aaagagcaca acaatggcgc aagacatttt tatgaatgat ttgaacgata
    18061 tatattttga gttggataaa aaggagcggg attttctgga gaagtccatt gaccaaattt
    18121 tccgcaaata ctactattca gcagtagaac ctgaaaccta tattacgcct gcaaatatat
    18181 tatcaagcct tgcagaaaag gaatcagcag gtaatactgt atttgttccc aaggttgaat
    18241 taattaaaaa gaaagatgtt cttacaaaaa tcaaatcatc gctgcactac gttaattctg
    18301 agaatcatta tatggtaact gattttgaaa ctgtgcttga gatttgtaag gaaagcttga
    18361 aggtaaatat aataggggca cctgaagata gtgaaagaaa aaggtttgag gatagacttt
    18421 ttttgaatga tagacattac ctcaatatta ttgtgctggt ggcaattaag cttggatata
    18481 tccagcttgt taagcaggga aagaaaaata tggcaataac cactgaaaag tcaaaagaac
    18541 tattaggcat gtcgatgaag gataagataa aaattcctgc aaaaggaata gttgaaaact
    18601 acgttagctc ttctgttctt gaaataccag agttaaaaga tataataaca aataatgcca
    18661 tatgggagat aattaaaaat cctataaagt ttgaagattt catgtattca gtaaatgaaa
    18721 aagttgggct tgacactgat atttttaata aaataataca tgataaagaa ctggagtacg
    18781 agcaattaga agacatggat gcaaataatc catatagcta catgctggct atgttcttga
    18841 tacatattga tgtgtatttg ttcacacctc tgggttatta tctgcagctc ataaagcctt
    18901 tttatggtaa tgtatataat attgcaattg aagtgggaga aattattgct gttttgagtg
    18961 gtaattatga acatgtacgg aacatgcttt tttttgtgcc ggaaaaattt gatttgacac
    19021 tgtttggaga ggagatttta ctggatggcg gaaaatcaga gcgaaggcag gaaattcctg
    19081 aaaagtatag tgataatagc ttcttaaagt gctttgaaat aagagagcaa tttattaacg
    19141 aggagttatt aaatctcatt gataactatg ggggcttcga cgaagacgat gacgacgatg
    19201 aggattatga tgacaatagc ttggatgaag attatgatga cgaagacgat gacgacgagg
    19261 attataatga tgaagattat gacaacgacg attacgaaga cgatgactat gatagagatt
    19321 atgaaaagga aggttttaca aagtctgaac ataaatatga taatataatt gatctatcca
    19381 attataaaaa agaggatagt agtattgaga gaaattatag aaaaaga
//
LOCUS       DAOSDD010000060        19309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1176_length_19309_cov_5.276202, whole genome shotgun sequence.
ACCESSION   DAOSDD010000060 DAOSDD010000000
VERSION     DAOSDD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19309
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1176_length_19309_cov_5.276202"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PK733_07315"
     CDS             <1..1025
                     /locus_tag="PK733_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ10385.1"
                     /translation="TKKNLKIAQSMPKGVKIIWGNLKTGENLELAIKDQDVVIHLAFI
                     IPPASERNPELAYEINVGGTRTIVEIIKSQKNPPKLIFTSTIAVFGNSQSLTPPRRVG
                     DPLNPVDNYGKQKIECEGIVKSSGLKWSILRLAAAPSIDWQRVDPIMYDVPLTDRIEF
                     VHPGDVGCALANAAGSKEIWGKILLIGGGPNCQMYQREFMKKALGMLGIGMLPDEAFG
                     KEPYHTDWMDTDESQRLLNYQGYSYDDFLKEQAKLFGKRAFFIKLLRPIVRWYLLCQS
                     PYYQEFMSGKVKSKRAGKCKICRKVALASADATARQIPKKKNNYKYKGNGSGRIKAHK
                     MWRRKN"
     gene            complement(1022..2626)
                     /locus_tag="PK733_07320"
     CDS             complement(1022..2626)
                     /locus_tag="PK733_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOJ10386.1"
                     /translation="MEKSNELKRLREIMSVFVKHGLKDGIAVISNPAQLRAAIEELGP
                     TFIKIGQVLSTRPDIISQPFIDEFQKLRDNVRPEQFDEIKAIIEGELGKPLDEIFLSF
                     EAEPVACASIAQVHVAELKNGESVVVKVRRPKIKEMMISDINILKRLARLLKITPQGA
                     VMNPVEIIDELGNTLKTELDFLNEAKNIKTFTENNKDTQYIKCPKVFNKYTTESLLVM
                     EYIDGIKISDKDQLEKKGYYLDDIALKLANNYMKQIFIDGYFHGDPHPGNILIAGDTI
                     AYIDFGMMGTLNKSMRQKYIFMLEGIATGNIDMITNAVLRIGKIKGNLNINKLRPEIE
                     EFYSNYAYLPVEEINIPEVVEELFKVCRRNSIAMPREITMLVKGVMTIEGVISDLSPD
                     INVMKVTVPFIRKLKLEEIDVKEDIVEQLGNLYLLSKQGLKMPLHIGELVNKALEGKL
                     KVNMEHTNLDKNVIELNKMANRLAFSIIVAALIVGSSLIIRADSGQGLRILGINNINI
                     MGLAGYLGAAVMGLWLLISILRSGRI"
     gene            2925..3860
                     /locus_tag="PK733_07325"
     CDS             2925..3860
                     /locus_tag="PK733_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOJ10387.1"
                     /translation="MIEKVYILGLGAVGSVYASMLFEMDPDCVEIIASKERIERYKKS
                     GVTVNGKLYPFNYNQPVQTAFPETSQRPADLIIIAVKQHHLRQAINDIRGFVGEDTII
                     LSLLNGITSEEIIGQEYGMDKLLYSFCVGTDAVREGRNTEYTKTGKIVFGENTNTEYS
                     GKVKMVKEFFDKAQIPYEIPENMIRQLWWKFMMNVGINQVSAVLKAPYGVFRKVGEAR
                     ALMEMACREVVNLSAYTGINLNDDDIKTSIEIIDTLSAEGKTSMLQDVEAGRKTEVEL
                     FAGTVIELGRKYGIATPVNDVLFKMICSLEKMYNL"
     gene            complement(4171..5220)
                     /locus_tag="PK733_07330"
     CDS             complement(4171..5220)
                     /locus_tag="PK733_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10388.1"
                     /translation="MKHNIGIIGYGGMANWHHRNSSRIEGLKCVAAYDIDPERLKVAE
                     EKGLRAFSSIDEFFKKGEFDIVLVAAPNNFHKELSIEAMNKGKHVVCEKPVAISSAEL
                     QDMIDASERNKVLFTVHQNRRWDKDFRIVRKVLEEGMVGKPYTIESRVHGSGGVIHGW
                     RAYKVSGGGMLLDWGVHLIDQIMYMIPEKVVNVFARMYNIKTPEVDDYFKVILTFESG
                     LNAQIEVGTYCLKPMPRWYVNGDLGSVYINGWDCIGGIIHAKQLAMEWEPEIVHTAAG
                     PTRTMAPRPKETLEEIQLPDVETDWADFYKNVIDVLDNNAELIVKPAEVMRVLKVMEA
                     CFSSAETGQAVNVNL"
     gene            5604..6797
                     /locus_tag="PK733_07335"
     CDS             5604..6797
                     /locus_tag="PK733_07335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ10389.1"
                     /translation="MGETKKIEEIDRNFIVPHSGISKFTYFDVTSGIFEISGFPWIES
                     NRLIGNGSHINDAQNSRPESNEAQSNETQNNGAENNESGKNESFCRLDIRWLDKLSEG
                     VKYLSWCTAGGVVRFASDSPAIAVKVELTSKGDMSHMPRSGSSGVDLYRGTGKNKIFV
                     RAAMPGSGQDKYEAVFEGFSSELCEWTLNLPLYNGIKKLEIGLLPGTKLEKPSGYTIE
                     KPVVFYGSSITQGGCASRPGNSYTHLICRWLDANMVNLGFSGSGRGEPEMAELISQID
                     MSAFVMDYDHNAPNLEHLEKTHENFFNIIRKAQPQLPVIFVTKPDFDREPETNRIRRK
                     IIYNTFKNAVDNGDQNVYFVDGETLFGTEDRDACTVDGCHPNDLGFMRMAENIYPTIK
                     KALRV"
     gene            7000..7698
                     /locus_tag="PK733_07340"
     CDS             7000..7698
                     /locus_tag="PK733_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10390.1"
                     /translation="MENLSLEKLPIEKLSTEKLSTEKLSTEKLSTEKLSIEKFFIERL
                     PVVMVCNYLDERNISELAKTYPIPEKFCIRNYRKGEENLWAEIETAAGEFKSEKEALE
                     RFKNEFGLYRDDMGKRCFFVINKESNKAIGTTTAWYNNNFNGQHYGRIHWVGIHPDFQ
                     GRKLAKPMLATAMMCLAKYHDKAYLTSQTTSYKAINMYLDFGFVPEIMSDECEKAWKI
                     IEDVLDRKILKHDI"
     gene            7775..8002
                     /locus_tag="PK733_07345"
     CDS             7775..8002
                     /locus_tag="PK733_07345"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ10391.1"
                     /translation="MDKLARDYRIKLIYIFGSYAKGEKREDSDLDIAMYFDIEVDGFV
                     KLDILDELVGIFNREDIDLEVMNNSQAYFDK"
     gene            8317..9345
                     /locus_tag="PK733_07350"
     CDS             8317..9345
                     /locus_tag="PK733_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ10392.1"
                     /translation="MATLKDIAEIAGVNVSTVSKALRDSSDINEDTKSEIRKIAKDLD
                     YKFKVSKRNPSNGVGIIGVICPEITSNYYSQIISIIEEEVKKEGYFCIIGFTNFETND
                     ERYYLKHLLNVDVDGIIFITESSNVGNTLSEYKKATSIPLVLVAQNTETKDFDCIKID
                     DDYGVRLAVEHFIQMGHKDIGYVGDELSNTRLDIFMKVMQENKMKINKKWIQVEEERF
                     EKCGYDLMSSLLKADDIPTAVLAAYDDIAIGAIKAVFDKGLKVPDDISVVGIDNVRIS
                     SYYQPELTTIAGPVEEMGKIAVKLLFKKVKDRQYNVVQNVRLSPRLVQRKSAKEFIEQ
                     NDSHNVSK"
     gene            9552..11252
                     /locus_tag="PK733_07355"
     CDS             9552..11252
                     /locus_tag="PK733_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ10393.1"
                     /translation="MCYLLGIDAGTTSFKGILIDEKGNTICIAKENYSLLKSGDNGGN
                     NDYGNNNNIVEFRAEQYWEVFKKLINKILAYSEVRPEEIAGLAIDSQGETLICLDENG
                     IPLRNAIVWMDNRSTEEASELREVFGIEKVYEITGQPELTSTWPATKMLWLSKHQKDV
                     FKNTKKYLLLEDYLNYRLTGKYVTEKSLVSSSLYFDIHKGQWWEEILNYIGITVDQLP
                     EIKESGQPIGVLTEEACRETGLSRNTIVVTGALDQLAGIIGAGNIYDNIVTESTGTCL
                     AMCMNAGSPPVYNEKLKIPCHYHAISDKYCLLFWSQTAGIILEWFKDNFYFLDNSINS
                     NSIRYNENNYIKKNEDSYSKQSGQNSYNSAKQNENKETKYGIYSHNNIYKIIDDEAAK
                     VPPGSEGLILLPHLNGSATPHFNPFATGVFYGVTLNHTRAHFARAIMEAVGYMLKEHI
                     ETAESFETEVKEIRSIGGGAKSTLWNQIKADITGKEIITLENTETASLGAAILAGLGT
                     GIFKDLESACKKCIKIKDRYYPDTSKTLIYQSSYEKYKELYNSVYPLFFSRGNTLCII
                     "
     gene            11509..12918
                     /locus_tag="PK733_07360"
     CDS             11509..12918
                     /locus_tag="PK733_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10394.1"
                     /translation="MYKAGFVGFGEVNTPRDVVDAKCNEAKELLVKNGFKLVTTGSVT
                     DDEKSTEAERAIRDLKGDDFDFLIVCLTGWIPSHTVIRVISEFKEKPMVLWGLAGNSK
                     DGNCSNCSNSENGSNAINASNMRLATTAAQAGTTAIRKPMEDMGYKFKYIYDFPDAQP
                     KIDEIITFAKAATTVAKLKKSKVGMMGYRDMRLYGTMFDGVSLKAKIGIEVEFFEMLE
                     IVQRMKNIDENKVNQLIEKILKEWDFQKEPNPDSLNFGVKAFLALKEKIEEDGYDAIS
                     LIDVDGMKKLLNFPPSLIFMLIANEMKKYTIPENDTLGAVTQLITGYATGQISAYMEF
                     YEFMPDRVLIGVPDYIPAEVIDGSLKVLPTSFGQLSEGVLNVSKVKTGKVTLARLGYS
                     NNEYVMHIVTGTAVEPRGWEEVGWEAPAPQLPSLEVILDTPIDDFAQKVMGQHYIVTY
                     GDNAGVLKDICKLMSIENM"
     gene            13152..14690
                     /locus_tag="PK733_07365"
     CDS             13152..14690
                     /locus_tag="PK733_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10395.1"
                     /translation="MNNASKTLLVGWASREITPERSVALCGQLYERISKYVRDPLTIT
                     ALALEEVDEKGKTCGHSIIVSCDLIAIYRTLLDAVRERLKHKTISINVNKVFIFATHT
                     HTGPVYGKSDDTTSCSVFTINDKNSSSGENIITAAECFEFLVDKICEAIEEAWSNRTP
                     GNISSAMERAEIGFCRMVMYRDGRSIMYGDTNTADFEGLLGSSDSAIEMLFFWNVVKE
                     LTGIVINVSCPSQVVEHMSFISADFWGETRKEIRKKLGDNIFVLPITGASGDQSPRDL
                     VRLKRDTVLKSSLTELNTSKPITDNSTPGWEMYDERGLYIIAERISSAVNNAYTKAEK
                     TIKSINEYKHVVEEILLPIRRVSMQDYKNALEQCNRIVRKYGIDENIDIESLYSNLHQ
                     TAIREEIFLPYGIVERYKMQQEQEFYKTEIHAVRLNDTVIVTNPFELYLEYGMRIRAR
                     CKAKQVIIAQLACDSAGYLPTERAVKGGGYSSIVASNIVGPEGGSLLVAHTVELVNNM
                     FRMQ"
     gene            14881..16623
                     /locus_tag="PK733_07370"
     CDS             14881..16623
                     /locus_tag="PK733_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10396.1"
                     /translation="MKGKIKRIGAFVIVIVFLFSFSTACTPEKNGDKISNSTQKTSSS
                     TQKTSSSTQKTSDNTKESNDTGKEGTNETQIGIPSSPPVKIKFMCSQTWAGSEYGYET
                     GLPRIKELEKRTGVEIEWEVYPKEDFYPVFRTRVAAAVDLPDTFEPIGVDDMNKWADE
                     GIFIPLDDLIEKYAPNIKKRYTVDYPGMYDLIRSGKDGKIYHLRHMHFFEGEDIEVIM
                     YREDWLNKVGFKKPPETTDEFYNFLKACQDKDVNGNGLKDEVLSSEGSYRLFLGFSSA
                     FGVPLYSPWVTDGWQEENGKVYYPWITPEAKEMYTYFNRLYKENLLWNDTPGIGSANL
                     REYYDELFANNAVSAMCHFPKNANDFHEGVKGIEGALYNILMPLIGPQGHGKVIYKTP
                     YFPYNSATVITRDSKNPDAVMRFLDYCMSEEGYLLLRHGEEGVHWERNNKGLVVKTQE
                     WEEITKNDPNASNKYGTVGCFPVWNDESFEQYSVKWKRPELKGIVTEILKMPSKSKFI
                     PALANPTEEEQNILNLHQAEITGYMWSTVNAFIRGDEPLSKWDDFVKRVNDLGLSKIQ
                     KIQQDRYNRAMNKR"
     gene            16849..>19309
                     /locus_tag="PK733_07375"
     CDS             16849..>19309
                     /locus_tag="PK733_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10397.1"
                     /translation="MKKLMILCLCISIILSYSLSGHVNAAPGKPLKEKFDIDEKFGQG
                     TRVDELLRQPYYSSILKVYEEKGYAAVENTEITLKATESVKSNKGDIPVKTGLYGKDE
                     ELLLWQEGYEWFEWEFDIPKTGLYEIEVEYYPVPGGGSPIRRSLAIDGQITFEEANNI
                     VFDRMWADDGEPKINNIGDEVRPRQKEIPQWDKVALTDSEGMYPGPFKFYFSEGTHII
                     RMNYIDQPVAFSRIIIRAPEEILSYEEVKKEYVQKNYSHGSDTTIFQSESTAILKSDP
                     TLRRESDADPKTQPLSRGSRVLNVIGDYRWRKGNQSITWEFTVPESGLYKIGLRAGQW
                     YTDGLPSYRQIAVNGKIPFAELKEYSFKFKHASWQTETLRDNNGEPFLFYLNEGKNTL
                     TMTVKMGPIRDFIHSLTEDSILLSNLIYKITMITGSDPDLFFDYNLDNRIPDLIEKFE
                     YFCDSLEEKARAMEDMSTKTPTWVHNKRSLIAAFKKMIEDPRSIAKKLNELVNAQSSL
                     GSEILALKEQPLVIDYFTVGPEKEKWEHIKSNIFEKLYTTVYNFLRSFWVDYDSIGNV
                     YEDSETETDSQGKNKEDGKDFGKNNKTNKLINIWTARGKEWGEIIKEMADETFTPKTG
                     IAVNMNILPASQLNAGAVNALMLAIASGRAPDVASAVGATSPVEFAIRDAAANLMEFD
                     DYDSVASRFLPGIMIPFEYNDGIYGLPETMGFRVLFYRKDIINELGIRLPDTWDDFYT
                     HVLPVLNQHQMEFYYPADITTFLFQKGGSFYKENGMKSALDSPEAYQAIKEMSELYTS
                     YGLPVAANFYNRMRTGEMPMGI"
BASE COUNT         6798 a         3070 c         3897 g         5544 t
ORIGIN      
        1 aaactaaaaa gaatctcaaa atagcacaat ctatgccgaa gggtgtaaaa ataatatggg
       61 ggaacttgaa gacaggagaa aatctggaat tggcaataaa agatcaagat gtagttatac
      121 atctggcatt tattattcca ccagctagtg agagaaaccc cgaattggcg tatgaaataa
      181 atgttggagg tacaagaaca attgttgaga taataaaatc tcaaaaaaat cctccaaaac
      241 ttatatttac atctacaata gctgtttttg gaaactctca gagtttgaca ccgcctcgga
      301 gagtagggga tcccttgaac cctgtagaca actatggcaa gcaaaaaatt gaatgtgagg
      361 gaatagtaaa gagttcaggc cttaaatggt ctatattaag gcttgcagct gctccgtcca
      421 ttgactggca aagagttgat cctataatgt atgatgttcc tttaaccgac agaattgaat
      481 ttgtacatcc cggtgatgtt gggtgtgcat tagcgaatgc agcaggttca aaggaaatat
      541 gggggaagat tttgctaata ggaggaggcc caaattgtca gatgtaccag agagagttta
      601 tgaaaaaggc actgggcatg cttggtatag gtatgctgcc tgatgaagcc tttggaaaag
      661 aaccttacca tactgactgg atggatacag atgaaagtca aaggctcttg aactaccagg
      721 gatactctta tgatgatttt cttaaggagc aggcgaagct ttttggcaaa agagcattct
      781 ttattaaact tttacgtcct atagtacggt ggtacctatt atgccaatcg ccatattacc
      841 aggaatttat gagcggaaaa gtcaaatcaa agcgtgcagg aaaatgcaaa atttgcagga
      901 aagttgccct ggcatccgca gatgccacag ctaggcaaat accgaaaaaa aagaataatt
      961 acaaatataa agggaatggt tctggaagaa taaaagcaca taaaatgtgg agaaggaaaa
     1021 actaaatcct tccggaacgc agtatggaga ttagcagcca gagtcccatt actgccgcac
     1081 ccagatatcc tgcaagtccc ataatgttaa tattgttaat tccaaggatt ctaagaccct
     1141 gccctgaatc tgcccggatt atgagagaag atccgacaat cagggcagct acaattatac
     1201 tgaatgcaag cctgttggcc atcttattaa gttcaattac atttttgtca agatttgtat
     1261 gttccatatt aaccttcagt ttaccttcaa gagccttgtt aaccagttca ccgatgtgca
     1321 atggcatctt caatccctgt tttgagagta ggtaaaggtt cccgagctgc tcaacaatat
     1381 cttccttcac atcgatttct tcgagtttaa gctttctgat aaaaggaaca gttaccttca
     1441 ttacgttaat atctggtgac agatcagata ttacaccttc aatagtcatt acacccttaa
     1501 ccagcatagt aatttctctg ggcattgcaa tactatttct tctgcatact ttgaataatt
     1561 cttcaaccac ttccggtata ttaatttcct caacaggaag atatgcgtaa ttactgtaga
     1621 actcttcaat ttccggtctt agtttattaa tattgagatt gccttttatc ttgccaatcc
     1681 ttagaacagc atttgttatc atatctatgt ttcctgttgc tataccttca agcatgaaaa
     1741 tatatttctg ccgcatgctt ttgtttagag tacccatcat tccaaagtca atgtatgcaa
     1801 tagtatctcc tgctatgagt atattcccgg gatgggggtc tccatgaaag taaccatcaa
     1861 taaagatctg tttcatataa ttattggcga gttttaatgc aatatcatcc agataataac
     1921 cttttttttc aagttggtcc ttgtcagata tttttatgcc atcaatgtac tccattacaa
     1981 ggagactctc ggtggtatat ttgttaaaaa cttttggaca tttaatatat tgtgtatctt
     2041 tattattttc tgtaaaagtt ttaatgtttt tggcttcatt taaaaaatcc agttccgttt
     2101 taagcgtgtt tcccaattca tcaataatct caacaggatt cattacagcg ccctgtggtg
     2161 taatttttaa aagccttgcc aatcttttta atatattaat atcactaatc atcatttctt
     2221 tgatctttgg acgtctgact tttacaacaa cactctctcc atttttaagc tcggcaacat
     2281 ggacctgagc aattgatgca caggccacag gctctgcttc aaaagataaa aagatttcat
     2341 caagaggctt cccaagttct ccttcaataa ttgcttttat ttcatcaaac tgttcaggcc
     2401 gtacattatc tcttaatttc tgaaattcat ctatgaaggg ctgacttata atatcaggcc
     2461 gggttgatag tacctggcct attttaataa aagtagggcc cagttcttct atagcagctc
     2521 ttaactgagc tggattactt ataacagcta tgccgtcctt taaaccatgc ttaacaaaaa
     2581 cggacataat ttctcttaac cgtttcagct cattactttt ttccataaaa cctccatgcc
     2641 gtcaatgtgc cgctatcaaa gcatttcact ggatttcaat tctactttaa tagtattcct
     2701 atttgacaac tacaataatg cactttgttg aacttattat attataccct gttatgaact
     2761 gcaagcaagc gatttgctcg tccccaataa atgagattga taatatcatt tttacttgat
     2821 agaatatgtg ataataatga aaagaacctc gaatgtataa aaacataaag tatttcaatt
     2881 gcggtgtagt tcagaatatg gaaaaacacg aagggagtaa aaaaatgatt gaaaaagtat
     2941 acatattggg attgggagca gtaggcagtg tttatgcaag catgttattc gagatggacc
     3001 ctgactgtgt tgagattatt gcatcgaaag aaagaataga gcgatataaa aaatccggtg
     3061 ttactgttaa tggaaagcta tatcctttca attataatca gcctgtacag accgcgtttc
     3121 cggaaacgag ccaaagacct gcagatttga ttattattgc agtaaagcaa caccacctgc
     3181 gacaggccat aaacgatata agaggatttg taggagaaga tacgattatc ctttccctct
     3241 taaatggtat aacaagtgaa gaaataattg gacaagaata tggcatggat aagcttctat
     3301 attcattttg tgtgggtacg gatgcggtaa gagaaggcag aaatactgag tacacgaaaa
     3361 cggggaaaat tgtatttggc gaaaatacca ataccgaata ttctggtaag gttaagatgg
     3421 taaaggaatt ttttgataag gctcagattc catatgagat tcctgagaac atgatacggc
     3481 agctttggtg gaaatttatg atgaatgttg gtataaacca ggtatcagca gtattaaaag
     3541 ctccctatgg agtgttccgg aaagttggag aagccagagc tcttatggaa atggcctgcc
     3601 gtgaagtagt taatttatct gcatataccg ggattaattt aaatgatgat gatattaaaa
     3661 caagtattga aattatagat actctttcgg ctgaggggaa aacctccatg cttcaggatg
     3721 ttgaagcagg aagaaaaaca gaagtagaac tatttgcagg aacagtaatt gaactgggca
     3781 gaaaatacgg aatagctact ccggtaaacg atgtgctttt caagatgatc tgttcgctgg
     3841 agaaaatgta caatctctaa ttatcctcca attattatcg gggcgtaaga agtggtaagt
     3901 gctaatttgg aaagcgatgc cttccgggat aatttttgat agcatcagga agatgtattc
     3961 cttacgagtc agcaaaaatt actctccgga aatgcacttt ccattggcaa tataacaaag
     4021 taggggcgca tttaaaattt ttgaagaaat atatttcaga agccatcgtt acggtactta
     4081 gagccttcac tactgcttct tctaataatt tcttcaaaaa taaaatgtgc acccacaaag
     4141 tagtattaat ttgctatctt aatctgatat ctacaaattc acattaactg cctggccggt
     4201 ttcggctgaa ctgaagcagg cttccattac cttaagtacc ctcattactt cagccggttt
     4261 aacaattagt tctgcattat tgtctaaaac atcaataaca tttttataaa aatcagccca
     4321 atcagtttca acatctggta attgaatttc ttcaagagtt tccttgggcc tgggtgccat
     4381 ggttcgtgtc ggtccggctg ctgtatgtac tatctcaggc tcccattcca tagctagttg
     4441 tttagcatgg attataccgc caatgcagtc ccatccattt atataaactg atccaaggtc
     4501 gccgtttacg taccatcttg gcataggctt aagacagtat gtccccactt ctatttgagc
     4561 attcagtccg ctttcaaatg ttaatattac tttaaaataa tcgtctactt caggagtttt
     4621 aatattatac atgcgggcaa aaacatttac taccttttca ggaatcatgt acattatctg
     4681 atcaattaga tgaactcccc agtccagaag cataccgcct cccgagacct tatatgcacg
     4741 ccatccgtga attacaccac cggaaccgtg aactcttgat tctattgtat aaggcttgcc
     4801 taccatacct tcttcaagta ctttacgtac aattctgaaa tccttatccc aacggcggtt
     4861 ttggtgtacg gtaaacaaca ctttattgcg ttcagatgca tcaatcatat cctgaagttc
     4921 tgcagaggat attgctacag gtttttcaca tactacatgc ttacctttgt tcattgcttc
     4981 gatagatagt tctttatgaa aattatttgg tgcagcaacc aatacaatat caaattcgcc
     5041 ctttttaaaa aattcatcaa tggatgaaaa ggccctcagc cctttttcct ctgcaacttt
     5101 tagtctttca ggatcaatat catatgctgc aacacatttc agaccttcaa tacgactgga
     5161 attcctatga tgccaattgg ccattccgcc atatccaata attccaatat tatgcttcat
     5221 tttagctatc cctccaaatt attaagtgct tgacatttat tgtattgcat ttttatattg
     5281 atgcaacata gaactgttat acttatttca atacaatata aggacattat acttaataaa
     5341 ttctttaaaa tccagcaata ttttacaaaa tacttagccg aaagcctatg actttagtca
     5401 atgggataga ggcttatctc gtgacttaaa attgcagagt ttctgataga ttataataga
     5461 atcaaatttc ctttatattt cgcagtgtgg tataatgtta accaatagat gagcaaacca
     5521 atgaaccaag tataggtgag caaaccaatg aaccaagtat aggtgagcta accaacgaac
     5581 cgagtatcag ggggtataac catatgggag aaactaaaaa aattgaagaa attgatagaa
     5641 attttattgt tccacatagt ggcatcagta aatttacata ctttgatgta acatcaggaa
     5701 ttttcgaaat atcaggattt ccttggatag aaagcaatag attaataggc aatggttcac
     5761 atatcaacga tgcgcaaaac agtagaccgg aaagtaatga agcccaaagc aatgaaaccc
     5821 aaaacaatgg agcggaaaat aatgaatccg ggaaaaatga atcattctgc agacttgata
     5881 taagatggct ggataagcta agcgaaggag taaagtacct ttcctggtgt acagcagggg
     5941 gagtcgtccg tttcgcttca gattctcctg ccatagctgt aaaagtggaa cttacgtcaa
     6001 aaggtgatat gtcacatatg ccgagaagtg gaagttctgg cgttgactta tatagaggga
     6061 cagggaaaaa caaaatattt gtcagagctg caatgccagg aagtggacag gataaatatg
     6121 aagctgtttt tgaaggattt agcagtgagt tatgtgaatg gactctgaat ttgcccttat
     6181 acaatggaat aaaaaagctg gaaataggct tgctacccgg aacaaaactt gaaaaacctt
     6241 ccggatatac aattgaaaaa cctgtagttt tctatggttc ttccataacc caggggggat
     6301 gtgcttcaag acccggcaac tcttacaccc atttaatttg cagatggctt gatgctaata
     6361 tggtaaattt agggttcagc ggaagtggca gaggtgaacc tgaaatggca gagttaatat
     6421 cgcagattga tatgagtgct tttgtgatgg attatgatca caatgcacca aacctggagc
     6481 atcttgaaaa aactcatgag aatttcttta atattataag aaaggcacaa cctcagctgc
     6541 ccgtgatttt tgtaacaaag ccggattttg atagagagcc tgaaacaaat agaattagaa
     6601 gaaaaattat ttataatacc tttaaaaatg ctgttgataa tggagatcag aacgtgtact
     6661 ttgttgacgg ggagacttta ttcgggactg aagataggga tgcttgtact gtagatggat
     6721 gccaccccaa tgatcttgga ttcatgcgta tggcggaaaa tatatatccc actattaaaa
     6781 aggcattaag ggtataagat atttaataac agatttaaaa tataatatat tgattattac
     6841 agatccattt ttgtatttta tagacattta aatgcaagaa aaggaagcag gaataaaatc
     6901 attgaagtaa tatggaagag gtcaactgcc aactacccaa taggcatcgt ggctttctat
     6961 attaatattc gtaaactaat ttgagaaggg tgataagata tggaaaattt atctttagaa
     7021 aaattaccta tagaaaaact atctaccgaa aaactatcta ctgaaaagct atctactgaa
     7081 aagctatcta ctgaaaagct atctattgag aagttcttta ttgaaaggct tccagtggta
     7141 atggtatgca attacctgga tgaaagaaac atatcggaac ttgctaagac atatcccatc
     7201 cctgaaaaat tctgtataag gaactataga aagggagagg aaaatctctg ggcagaaatc
     7261 gaaacagccg ccggagagtt taaatctgaa aaagaagctt tggaaaggtt caaaaatgag
     7321 tttggattat atagggatga tatgggaaaa agatgttttt ttgtaataaa taaagagagc
     7381 aacaaagcta ttggaacaac aacagcctgg tacaataata acttcaatgg acagcattat
     7441 ggacgtattc attgggtggg cattcacccg gatttccagg ggagaaagct tgcaaaaccc
     7501 atgctggcca ctgctatgat gtgtcttgct aaatatcatg ataaagccta tcttacatcc
     7561 caaaccacaa gttataaagc cataaacatg tatttggact ttggctttgt tcctgaaata
     7621 atgagtgatg agtgtgagaa agcatggaaa atcatagagg atgttcttga ccgcaagata
     7681 cttaaacatg atatttaacc ttcctcgccg agaattctca cactcttgta agtaaagaaa
     7741 tattgatgaa gtagaaaaag tctttaacaa gcacatagat aagttggcac gcgattatag
     7801 aataaaactt atttatatat ttggctctta tgccaaaggg gaaaaaaggg aagatagtga
     7861 tttggatata gctatgtatt ttgatattga agtagacgga tttgtaaaat tagatatatt
     7921 agatgagcta gttggaatat ttaatcgaga agatatagat ttagaagtta tgaataattc
     7981 tcaagcttac ttcgataagt aaaattttac aattgccctt tgagctatag cttcagacat
     8041 ctaaagactt aatctggccc tgagctgtag ctacctgata agcaacaggc taacaagcaa
     8101 cagtgaatca atctggaaat ttttaaggaa cttgatttca tggatcattg catgacctag
     8161 aaaatccact tcaattttcg aaaatacctc ttgctatttt tctataagtt cgataaaatt
     8221 gtaatataat atctgacaac agtgatcggc aggtattgtt tagccacagc cgcgataacc
     8281 caaaatacaa atacacataa aagaaaggtg gaatatatgg ctacattaaa agatattgca
     8341 gaaattgcag gagtaaatgt atcaacagtc tcaaaggcat taagagacag ctctgatata
     8401 aacgaggata ctaaatcaga gataagaaaa attgcgaaag atttggatta taaattcaaa
     8461 gtatccaaaa ggaacccatc aaatggagtc gggattattg gggtaatatg ccctgaaatc
     8521 acaagcaact actactctca gattataagt ataattgagg aagaggttaa gaaagaaggt
     8581 tatttttgta ttattggctt tacaaatttc gaaacgaatg atgagagata ttatttgaag
     8641 cacttactta acgttgatgt tgatggaatt atctttataa ctgaaagctc taatgttgga
     8701 aataccttat ctgaatacaa aaaggccact tccatacctc ttgttttggt agcacaaaac
     8761 actgagacaa aagattttga ctgtataaaa attgatgatg actacggagt aaggcttgct
     8821 gtggagcact ttattcaaat ggggcataag gatattgggt atgtggggga tgaattgtcc
     8881 aatacaaggc ttgatatatt tatgaaggtt atgcaggaaa ataagatgaa aataaataaa
     8941 aagtggatac aggtggaaga agagaggttt gaaaaatgtg gctatgattt aatgagcagc
     9001 ttgttaaaag cggatgacat tccaactgct gttttagcag cttacgatga tattgccatc
     9061 ggagcaataa aagcagtttt cgacaaaggt cttaaggtgc ctgatgatat ttcagtagta
     9121 gggatagata atgtaaggat atcatcatat taccagcctg aactcacaac catagcaggt
     9181 ccggtagaag aaatgggaaa aatcgccgta aaactcctat ttaaaaaggt aaaagacaga
     9241 caatataatg tagtgcaaaa tgtaaggctt tcaccaaggc tagtgcagag aaagtcggca
     9301 aaagaattca tagaacaaaa tgactcacac aatgtttcga aataaattat acataataaa
     9361 gtttaatcca aaaataattg cgaaaatatt acgaaaaggc attgacaact aatcacatct
     9421 agattacaat gtatactgta actaaatatt atcatctgca atgcaaaaat gtgaaacagt
     9481 acatagtaat atagtttgcg aaaaatattc atcaggcaga gggtatcttt attttgtgga
     9541 ggcttgaaac agtgtgttat ttgttaggta ttgatgccgg tacaacttca tttaaaggaa
     9601 ttttaattga cgagaaaggc aatacaatat gtatagctaa agaaaactac agtctgttaa
     9661 aatctggtga taatggcggt aataatgatt atggcaacaa taataatata gtcgaattca
     9721 gggctgaaca gtattgggaa gtttttaaaa aactgataaa caaaattctc gcatactcag
     9781 aagttaggcc cgaggaaata gcaggacttg ccatagatag ccaaggtgag acattaatat
     9841 gccttgatga aaatggtata cccttaagaa atgccattgt gtggatggat aatagatcta
     9901 cagaagaagc aagtgaactg cgagaagtat ttggcattga aaaggtgtat gagataacag
     9961 gccaaccgga gctgacttct acttggccgg caacgaaaat gctttggcta agtaagcatc
    10021 aaaaagatgt ttttaaaaac acaaaaaaat atcttttgct tgaggactat ctcaattacc
    10081 ggttaacagg aaaatatgtc actgaaaaat ccttagtttc ttcatcttta tatttcgata
    10141 ttcacaaagg gcaatggtgg gaagaaatac tcaattacat aggaataaca gttgatcaat
    10201 tgccggaaat aaaggaatca ggacagccta taggtgtatt aaccgaagaa gcctgcagag
    10261 aaaccggcct aagccggaat acaatagtag ttacaggcgc tttggatcag ttggcaggta
    10321 taattggagc aggtaatatt tatgataata ttgtaacaga atctaccggc acctgcctgg
    10381 caatgtgcat gaacgcaggt agcccgcctg tatataatga aaaattaaaa ataccctgtc
    10441 actatcatgc aatttcagat aaatattgtt tactattctg gtcccaaaca gcaggcatta
    10501 tactagagtg gtttaaagat aatttttatt ttctggataa tagcattaat agcaatagta
    10561 tcaggtataa tgagaataac tatatcaaaa agaatgaaga tagttatagt aaacagagtg
    10621 ggcagaatag ctataatagt gctaaacaga atgaaaacaa agaaacaaaa tatggtatat
    10681 atagccataa taatatatat aaaataattg atgatgaagc agcaaaagtc ccgccgggat
    10741 cggaaggtct tattctactc ccacacttga atggaagtgc tacacctcat tttaatcctt
    10801 tcgcaacagg tgtgttctat ggggtgaccc taaaccatac tagagctcat tttgccagag
    10861 ctattatgga agcggtaggc tatatgctaa aagaacatat agaaactgca gaaagctttg
    10921 aaacagaggt taaggaaatc agatccattg ggggaggtgc taaaagcaca ttatggaatc
    10981 aaattaaagc agatattaca ggaaaagaga taataacact tgagaatact gaaacagcct
    11041 ctctgggagc agctatactt gcagggttag gcacaggaat ttttaaagat ttggaaagtg
    11101 cctgtaaaaa atgcataaag attaaggaca ggtattaccc tgatacatct aaaacactaa
    11161 tatatcaatc cagctatgag aaatataaag agttatataa cagcgtttat cctttgtttt
    11221 ttagtagggg aaacacacta tgcataatat aaataaaaac cttagtgcta acagcaaaaa
    11281 tacagatatt aaccaaggta ataaatgcta cctttatgct agagtttttt gtcctagatg
    11341 tggagggttt aatatttgta aacatcgctt actttctcgt aaatatttat gcaatgattg
    11401 tggatataaa tggataagct aattgttata tagtataagt ttagtgaaga tgtgtgtaaa
    11461 tacaaaaatg agcaaaatgg aaaaaacttg gaaagtggga atctaaaaat gtataaagca
    11521 ggttttgtag ggtttggcga agttaataca ccaagggatg tagttgatgc aaaatgcaat
    11581 gaagcaaagg agttgcttgt aaagaacgga tttaaactgg taactacagg ttctgttact
    11641 gatgatgaaa aaagtacaga agcagaaagg gcaatcaggg acttaaaggg agatgatttt
    11701 gattttctga tagtctgttt aactgggtgg attccttcac atactgttat aagggtgatt
    11761 agtgaattta aagaaaaacc tatggttttg tggggattag ctggaaacag taaggatggc
    11821 aattgtagta attgcagtaa tagcgaaaat ggaagcaatg ctatcaatgc cagcaatatg
    11881 agacttgcta ccacagcagc ccaggcagga acaactgcta tcagaaaacc tatggaagat
    11941 atgggttata agtttaaata catatatgac ttcccggatg ctcaacctaa aattgatgaa
    12001 ataatcactt ttgcaaaagc tgccactaca gttgcgaaac taaaaaaaag caaggtcggc
    12061 atgatgggtt accgggatat gaggctttat ggcactatgt ttgacggagt gtctcttaaa
    12121 gctaaaatcg gaattgaagt tgaatttttc gaaatgctgg aaattgtgca gagaatgaaa
    12181 aatattgatg aaaacaaggt taatcaactc atagaaaaaa ttcttaaaga atgggatttt
    12241 caaaaagaac ctaatccaga ttctcttaat tttggagtaa aagcttttct cgccttgaaa
    12301 gaaaaaatcg aagaggatgg atacgatgct atatcactaa tcgacgtaga tggtatgaag
    12361 aagctcttaa attttccacc ttcattgata tttatgctta ttgccaacga aatgaaaaaa
    12421 tacactatac ctgaaaatga tacattagga gctgtaaccc agttaataac aggttatgct
    12481 accgggcaga tttccgctta tatggagttt tatgaattca tgccggatag agtacttatc
    12541 ggggtacctg attatatacc ggctgaagta atagatggat ctctaaaagt acttcccaca
    12601 agttttggac aattaagtga aggagtgtta aatgtatcaa aggtgaaaac aggcaaggtg
    12661 acgttagcaa gattgggtta cagcaataat gagtacgtaa tgcatatagt aaccggcacg
    12721 gcagttgaac caaggggttg ggaagaagta ggctgggagg cccctgcacc acagcttcca
    12781 agtttagaag taatacttga tacgccgata gatgattttg cccagaaagt aatgggccag
    12841 cactatattg taacttatgg ggataatgcg ggggtgctga aggatatatg caagctgatg
    12901 agtatagaaa atatgtaaat tacagaaata tgtaaaaatt gattatatgc tactaaaaat
    12961 gttataattg agttcaaaca atgacactca cctttaggtt tatttggttg tcgtgaactt
    13021 aattatacca gaaaagaggg ggtcattgtt ttttatttca aagaaacctg aaacccttga
    13081 taaatagaac tttgaatcta tttaatattc aaatagatta acactacgat tattatatga
    13141 gaggtaaaat tatgaacaat gcatcaaaaa cattattagt tgggtgggct tctcgcgaaa
    13201 taacgccgga aagatccgtt gcattatgtg gacagcttta cgaaagaatc tcaaaatatg
    13261 ttagagatcc tttaacaatt actgctcttg cactagagga agttgatgaa aaaggaaaga
    13321 cttgtgggca ttcaattata gtttcatgcg atcttatagc tatttataga acattgttag
    13381 atgcagttcg tgagagacta aagcataaaa caattagcat taatgttaat aaagtgttta
    13441 ttttcgcaac tcatactcac acaggtccag tttatggaaa aagcgatgat accacaagct
    13501 gtagtgtctt tactatcaat gacaaaaact ccagctctgg ggaaaatata ataactgcgg
    13561 cagaatgttt cgagtttctt gttgataaaa tctgtgaagc tatcgaagaa gcttggagta
    13621 ataggacacc cggaaatatt agttcagcaa tggagagggc agaaataggg ttttgtagga
    13681 tggttatgta cagggatgga agatcaataa tgtatggtga tactaatact gctgattttg
    13741 aaggtttact gggttcaagc gactccgcta tagaaatgct gtttttctgg aatgttgtca
    13801 aagaacttac aggaatagta atcaatgtat cttgtccttc tcaagttgtt gagcacatgt
    13861 cttttatatc agctgatttt tggggagaaa ccagaaaaga gatacgtaaa aaattaggag
    13921 ataatatatt tgtattacca ataactggtg cttcaggaga ccaatcacct cgtgaccttg
    13981 taaggttaaa aagggatact gttttaaagt ccagcttaac tgagttaaat acctcaaagc
    14041 caataacaga taactcaaca cctggatggg aaatgtatga tgaaagaggt ctatatataa
    14101 ttgcagaaag aattagctct gctgttaata atgcttatac taaagcggaa aagacaataa
    14161 agagtataaa tgaatacaag catgttgttg aggaaatatt gttaccaata aggcgggtaa
    14221 gcatgcaaga ctacaaaaat gcacttgaac aatgtaatag gattgtacgg aaatatggca
    14281 ttgatgaaaa tattgatatt gaaagtttat atagtaattt acatcaaact gcaatcagag
    14341 aagaaatatt tttaccatat ggaatcgtag aaagatataa aatgcaacaa gagcaggaat
    14401 tttacaaaac tgagattcat gctgtaaggt taaacgatac agttattgta actaatccat
    14461 ttgagcttta tcttgaatat ggaatgagaa tccgggcacg atgtaaagct aagcaggtta
    14521 ttattgctca acttgcatgc gattctgcag gttatttacc aacagaacgt gcagtaaaag
    14581 gaggagggta tagctcgata gtagcgagta acattgtcgg accagaaggg ggctcattac
    14641 ttgtagcaca tacagtggaa cttgtaaata atatgttcag aatgcaatga aaatgaattt
    14701 gcagtataaa aaacataaat attatattta gttaatacat tttaaattgg cagttgctga
    14761 actagatagt aaagaaagga gatagagttt acttaataaa taagtagttt tataaaggct
    14821 ttgaataaaa attgtaaaca atagccaata ataacttgtg aaagagagga atggtattaa
    14881 atgaaaggta aaattaaacg tataggtgct tttgttattg ttatagtatt tctttttagt
    14941 ttttccactg cctgtacacc agaaaagaac ggtgacaaaa taagtaatag tacacaaaaa
    15001 acatctagta gtacacaaaa aacatctagt agtacacaaa aaacatcaga taatacgaaa
    15061 gaatcaaatg atactggaaa agagggcaca aatgaaactc aaataggaat tccttcaagc
    15121 ccacctgtaa aaataaagtt tatgtgttct cagacttggg ctggttcaga atacggttat
    15181 gaaacaggtc tgcctagaat aaaagaatta gaaaaaagga caggtgtaga aatagaatgg
    15241 gaagtatatc cgaaagaaga cttctaccca gtatttagaa ctagagtcgc agcagctgtc
    15301 gatttgcctg atacgtttga gcctattggg gtagatgata tgaacaaatg ggcagatgaa
    15361 ggaatattta taccactaga tgatttaata gaaaaatatg ctccaaatat aaagaaaagg
    15421 tatactgtcg attatccagg aatgtatgat cttataagat caggaaaaga tggtaaaata
    15481 tatcacctac gccatatgca tttttttgaa ggtgaagata ttgaagtaat aatgtataga
    15541 gaggattggc tgaataaagt aggttttaaa aaaccgcctg aaacaacaga tgagttttac
    15601 aactttttga aggcctgcca agataaggat gtgaacggca atgggttaaa ggacgaagtt
    15661 ttatcttcgg aggggtcata cagattgttt ctagggtttt catcagcctt tggagtacca
    15721 ctatattcgc catgggttac agatggatgg caggaagaaa acggaaaggt atattatcca
    15781 tggataacac ctgaagctaa agaaatgtat acatatttca atagactata taaagaaaac
    15841 ctattatgga atgatacacc cggaatcggt agcgcaaatt taagagaata ttatgatgaa
    15901 ttatttgcta ataacgctgt atcagctatg tgccatttcc ctaaaaacgc aaatgatttt
    15961 catgagggag taaaagggat tgaaggtgca ctatataata ttttgatgcc acttatagga
    16021 ccacaaggac atggaaaagt tatatataaa actccttact ttccatacaa ttctgccact
    16081 gtaatcacta gagatagtaa aaatcctgat gctgtaatga gatttcttga ttattgtatg
    16141 agtgaagaag gatatttatt gctaaggcat ggtgaagaag gtgtacactg ggaaagaaat
    16201 aacaaaggat tagttgttaa gacacaagag tgggaagaaa ttactaaaaa tgatcctaat
    16261 gcatcaaata aatacggaac tgtaggttgt ttccctgtgt ggaatgatga aagctttgaa
    16321 caatattccg ttaaatggaa acgaccagaa ttaaaaggta ttgttactga aattttgaaa
    16381 atgccttcaa agtctaagtt tattcctgca ttagctaatc ctacagaaga ggaacaaaat
    16441 attcttaatt tacatcaagc agaaattact gggtatatgt ggagtacagt taatgcattt
    16501 ataagaggag atgaaccttt atcaaagtgg gatgattttg taaaaagggt taatgactta
    16561 ggattaagta agattcaaaa aatacagcag gacagataca atagagctat gaataaaaga
    16621 tagtatatgg tattaaaatt attggtagca ttaaaatctg cgaatagata ataaaagaga
    16681 atgcaaagta gagtggaaga aagttcatac cttccactct aaacacgcaa agaaagggct
    16741 gatggaatga cttgcaaaaa gcgccggcta tttaataaaa aaccctatag gcttaaaact
    16801 aaaactatac ataggtgtat ccgccctaat ttatccccaa gcttatttat gaaaaaactg
    16861 atgattctat gtctatgcat atcaataatc ctttcatact cactgtctgg tcatgtaaac
    16921 gccgccccag gcaagccttt aaaagaaaag tttgacatag acgaaaaatt cggtcagggt
    16981 acaagagtag atgagctttt gagacagcca tattattcat ctatactcaa agtatatgaa
    17041 gaaaagggat atgctgcggt agaaaatacc gaaattactc taaaagcaac agaatcagtg
    17101 aaatcgaata aaggtgacat acctgtaaaa acgggtttgt atggaaaaga tgaagaactt
    17161 ctcttatggc aagagggata tgaatggttt gaatgggaat ttgatattcc aaagactggt
    17221 ctttatgaaa ttgaggtaga atattaccct gtacccggag ggggatcccc aataagaagg
    17281 tcacttgcta tagacgggca aataacattt gaagaagcca ataatattgt ttttgaccgg
    17341 atgtgggcgg atgatggaga acctaaaatc aataatatag gggatgaagt aaggccaaga
    17401 caaaaggaga ttccacagtg ggataaagta gctttaacag acagcgaagg aatgtaccct
    17461 ggtccgttta agttctactt tagtgaagga actcatataa ttcgtatgaa ttatatagat
    17521 cagcctgtag ccttttcaag gataataata agagctcccg aagaaatact ttcgtatgaa
    17581 gaagttaaaa aggagtatgt gcaaaaaaac tattcacatg gaagcgacac tactattttt
    17641 cagtcagaaa gtactgctat tttaaaaagt gatcctactt taagacgtga aagtgacgca
    17701 gaccctaaaa ctcaaccttt atccaggggc agccgtgtac ttaatgtaat tggagattat
    17761 cgctggagaa aaggaaatca atctataacc tgggaattta ccgttcctga gagcggttta
    17821 tataaaattg gactaagggc tggtcaatgg tatactgatg gtcttccatc atacagacag
    17881 atagcggtaa atggtaaaat accctttgcc gagcttaaag aatatagctt taagttcaag
    17941 catgcaagct ggcagacaga gacattaagg gataataatg gtgaaccatt cctgttttat
    18001 ctgaatgagg gaaaaaatac tttgaccatg acggtaaaaa tgggaccaat acgggacttt
    18061 atacattcat taaccgagga ctcaatttta ctatcaaacc tcatatataa aattacaatg
    18121 ataactggca gtgatcccga tttgtttttt gattataacc ttgataatag aataccggat
    18181 cttattgaaa aatttgaata tttttgtgat agcctcgaag aaaaagctag ggcaatggaa
    18241 gatatgtcaa caaagactcc tacctgggta cataataaaa gaagccttat agctgcattc
    18301 aaaaagatga tagaagaccc cagaagtatt gcaaagaaac taaacgaact ggtaaatgcc
    18361 cagagcagtt taggttctga gatattagct cttaaagaac agcctcttgt aatagattac
    18421 tttacggttg gaccggaaaa ggaaaaatgg gaacatatta aatccaacat atttgaaaag
    18481 ctttacacta ctgtatacaa ctttttacgt tctttctggg ttgattatga cagcataggg
    18541 aatgtatacg aagattcaga aactgaaaca gacagccaag ggaaaaacaa agaagatgga
    18601 aaggattttg gaaaaaataa taaaaccaat aaattgatta atatatggac tgcacggggt
    18661 aaagagtggg gagaaattat caaggaaatg gcagatgaaa cttttacgcc taaaacaggg
    18721 attgcagtaa acatgaacat cctgcctgct tcacaactca atgctggtgc tgtaaatgca
    18781 ctaatgcttg caatagcctc aggcagagcg cctgatgttg ccagtgcagt aggtgctact
    18841 tcacctgttg agtttgccat aagggatgca gcagctaacc ttatggagtt tgatgattat
    18901 gacagcgtag cttccagatt tcttcccggt attatgatac ctttcgaata taacgacggg
    18961 atatatggtc ttccggaaac aatgggattt cgagttttat tttacagaaa agatataata
    19021 aatgagcttg gaataagact tcctgataca tgggatgatt tttacacaca tgtacttcct
    19081 gtacttaatc agcaccagat ggagttctat tatccggccg atataacaac attcctattt
    19141 cagaaaggcg gaagttttta taaggaaaac ggtatgaaat ctgctcttga cagccctgaa
    19201 gcataccagg ccatcaaaga aatgtcagag ctttatacaa gctatggcct tcctgtagcc
    19261 gcaaattttt acaaccgcat gagaaccgga gaaatgccta tgggaattg
//
LOCUS       DAOSDD010000061        19256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1182_length_19256_cov_5.412947, whole genome shotgun sequence.
ACCESSION   DAOSDD010000061 DAOSDD010000000
VERSION     DAOSDD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19256
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_19256_cov_5.412947"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="PK733_07380"
     CDS             complement(<1..1987)
                     /locus_tag="PK733_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10398.1"
                     /translation="MNWDHFQTYNDAPTHAFETMCNQLFELWCRRNYANNIKSFFFVN
                     GAGGDGGVEAYTVTKDDSIVAVQSKWFKSSIEDSQFRQIENSVKTAIKIRPLIKKYII
                     CVPRDLSSSKMSKGNKISEKTESSRWEELKKELLISLPNLEIELWNSTRILTELQYPE
                     CSGILRYWFEKSEISQDIIDLSFEKQKSSWLSQRYLPDLHVNGAINQHVNLLIGSHNQ
                     RNLVCVKLKELKYLCNNFLLELDDCVSLFNKKDLTEIANQVSIKVKRTISHIDVFIES
                     LCSDKLICTHIEELLSSYDFDDLLGCLEKCSKNKKPIHFSEIKKSIHKIIDSSADENI
                     ITTISQLKEDTLLVLGDPGTGKTHGIANVVKHFNMSKNHVSILIRAKSISNKATWHEI
                     LTRALGLSNAWSEGEIWQGLSSLSCRNEHRQVLEKNNPVDGIYIMPKVLICVDGIDEL
                     RPYDTWFNLIGETEAICKKYPRVKFCFTSRPYVFNVTSPDKRIMSSVLRSTSDGDAPV
                     SSLFDAYIKAYDVTLNGNEWLRWSIKTPLALKIFCELYNHKEVEAIEQSSITITNLLN
                     KKIEKIDSEFRSIFNYGYDANDNIVKKSLLMLSEYFYLKNQIEKNDLIKMISQIDSLK
                     IAAPQECRDLIKYLEDYGLLYSVVIYPTSFLEEPKIYY"
     gene            complement(2061..2294)
                     /locus_tag="PK733_07385"
                     /pseudo
     CDS             complement(2061..2294)
                     /locus_tag="PK733_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3054..4991)
                     /locus_tag="PK733_07390"
     CDS             complement(3054..4991)
                     /locus_tag="PK733_07390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOJ10399.1"
                     /translation="MAMSIGINSSDLLTPEQLDSHIKIYAGPGAGKTHFLVENVKNIV
                     TTYPLVVQSRERKVLCITYTNAAVDEIKHRLDRFSSSVEAHTIHGFIIEHIIKPFQED
                     LRQIMREDFGITVKGKSKITSQVEGMGILHGIDKTDIFEFIKSAVGDTEELSYSKKIL
                     GEVEVDLAAYLADKTAKLRASRQINNNHIIPIKQYTWSKVRKLTHNEILYFGYRILQH
                     NHTALYATRVKFPFIFVDEFQDTNPLQTLLIKLIGSKSTVIGTIGDVAQSIYSFQGAR
                     PSQFLALNIDGNRASSEYAISGNRRSTANVVNLCNFLRKADSTVTQNSEKCYNSDEEK
                     QSAEKTKIHIISGEDAAAKQLINSAIADGAVVLTRTWAAAFSYIQDINGEQERSLKAI
                     YNAYYTSPIDIRSDIVEHNYVTWVRAFKFIFRLWNGYKTGALVEIIAALALITDFDTK
                     KLTPKILTQLKRLTDEVFGDLSVEALTVDIINGFSSQLNEEKYTELVSVIGEEQFTIP
                     IFDDEDRDEIKTNVSLLSWYTSHKLFTEVFSEDSRYMTVHQAKGREWEKVIVSLEPNS
                     RRDRDNTTLTSMFSNPQLLNEENAEEFTRMYYVACSRAKVDLYIHLKKASDVTALQSA
                     LDNYNQVHEGNRRIDYEILPL"
     gene            complement(4970..6982)
                     /locus_tag="PK733_07395"
     CDS             complement(4970..6982)
                     /locus_tag="PK733_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10400.1"
                     /translation="MYIQRISIKNYRNYKDFTMNFHKGLNVIIGANNSGKTGLLTAVR
                     LLREPYVCADDFNKNELLGFGTKYREDAPQIAIEYTICHTISEEDTEDESIIRLLPFL
                     GMNELTEFQQEADGATNYNITAIIKAVCTLDEKALSDYKREVAKVADNDFGKYMIILK
                     SFENRYSWSYYNAASNTAADKKAATEIFDIRFIGAERTSDDVHKETKREIDAFAKDQG
                     NATAIAELRDSVALDMKAILQSVLDKLEKLFEGENNDIGLSHGNVSISQNLRPNVSVG
                     DSYITEVRDTKTDYIVPLSYNGLGYNNLINIYMLIKLTEIRPGRDFRILLLEEPEAHL
                     HPALQYKLFKYLRQLDQEDNLNQQIFVTTHSSNISAVAGIDNMFMIAYDRNGDCSDCK
                     EQSLSVQFTDSEGAEKHKEKAKKHLTKFLDVTRSDMLFADKVILVEGIAERLLMPLFM
                     EKCGCSYEDEHIAVVEIGGKHFEHFVELFNGNAVNKKVLCITDCDFKWIADGKTEKIE
                     AYQTTAPEHIQKLKNRFPIDNFHISTQSIGGRTFEDELLLSNFDNPEISLKIFKMPTS
                     QVISDFVDEHGFDFAKWETHLPELDGRSQPLVKKRLDAYKQAIQDDTAHKTEYENLFF
                     ANLFLHYAKGCKGDVALNILVDEGLAKDLTVPAYIQEGLKWLCLSE"
     gene            complement(7332..7526)
                     /locus_tag="PK733_07400"
     CDS             complement(7332..7526)
                     /locus_tag="PK733_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10401.1"
                     /translation="MALEAIIITIELASRFLADHINGDKYFRIHRENHNLDRARCQIK
                     LAQDMICKYDEMCNIVQRYQ"
     gene            complement(7508..7693)
                     /locus_tag="PK733_07405"
     CDS             complement(7508..7693)
                     /locus_tag="PK733_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10402.1"
                     /translation="MILNLFGDTIHFAANTAMEVETDLSKVELDMKKYEEFTKGFMIA
                     SKEFLIKEELDTWHWKL"
     gene            complement(9003..9341)
                     /locus_tag="PK733_07410"
     CDS             complement(9003..9341)
                     /locus_tag="PK733_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ10403.1"
                     /translation="MIKPIFRKVSECKDKGLGYIISGDEAEIIESNIVTVKKGVTTDE
                     SSHHNEEEVYIILSGKGRVKVGDIEKEVGRDTVIYIPRNAIHQSTGLSDEDFVFLCVA
                     IYFDKTPKEQ"
     gene            complement(9405..10748)
                     /locus_tag="PK733_07415"
     CDS             complement(9405..10748)
                     /locus_tag="PK733_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ10404.1"
                     /translation="MSKNIMFGVAKDIVTPCVETTMLGFGTVFGKSFTGIHDDLYVKC
                     LLLKDGKQILVLLSLDLCFHDDSLPISIGEYLSEKYQIPYNNLLISYTHTHFGPCLKG
                     YDFIFHSPEYESFLLQRIKDCINRAFLNIYEGTIEYTSILGEWNISRRLKVDDVMQRA
                     ILPNPDGECDKNMYLLKLTDKSGNVKVIAINFACHPSNLDSYRILSSEYPGRLCHLIE
                     ANNYGCTAVFFQGFGGDSKLKIGAKSSKFHPISYEDCNEVASSMALKIQSSLINKKWE
                     VIQPSLSSRMFQIKIPLEVYPLSFFEKELQNFSDNSKVPFDKNMVKDYESNGSLLMWS
                     NAEYVVNNYDRLHDYLILNCGIIRIGKGHYIFTIGGEPSYDVKKVLQELLPEANVLYF
                     GYNDAIAYVPSDKMLEEGGYEAGDGSVTEYRMKGKFKKGIDNYFLEGFRNALNQL"
     gene            complement(10831..12462)
                     /locus_tag="PK733_07420"
     CDS             complement(10831..12462)
                     /locus_tag="PK733_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10405.1"
                     /translation="MRKRSIAMFTLIIFLLSLLLGCNAPTKNNQKTTAGQETDISKTD
                     ENFNAIGYPIVKKPITVTSMFAPGTYDGDPKEMSYWQKLEEISNIQIKWNMISSSEPT
                     AIQLFFSAGDFPDFFHAYVDNERQFIYGVEGGMFYDYSDLIYEYMPNLVSWFKKYPEA
                     EKVIRQLNGAIYTLPKIQVHSTYSIGQMFYRTDYLKKLNLEIPKTVDDFYNVLKAIKD
                     SGLTGGYAPFLPLNKGTFEYHTEAFLFSAFGDAHDYDFADDGKGKVVYNRISEQYRRY
                     LEYANKLYKEGLLENELFTIDSDTSSGRIKAGQAAFMTSADMLAESDFPDGKINIDCL
                     APLTSQYTSERKVKGYIGVSSSAAAINKNCKYVRELLRMFDMNYTEDEVVPGSGLNCL
                     SQNIGLEGVNWKYTNNEKSHISFLQPDDWDSNVWLWITKYASWNQFYSAWVTYATTGG
                     ANNIARETGMLKNNLPYAKPSFPDTLIKVTEEENNVIANKLTDINNFVAQARAQFITG
                     IEPLSKWDAYVAKVKEMGIDEILEIKQKAYDRWNE"
     gene            complement(12541..13077)
                     /locus_tag="PK733_07425"
     CDS             complement(12541..13077)
                     /locus_tag="PK733_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10406.1"
                     /translation="MYFSGGLIPSYLLIKKLGLLDTRLILIITGAISVYNMIICCTFF
                     QNIPRDLEEAAYIDGCSTARTFVQIIIPLSKALIGVMTLYYAVGHWNSYFTALIYISD
                     KAKKPLQLFLREILITEQMSAAMAEKIGGERLYLMEQLKHLLKYSVIIVSSLPVLILY
                     PFLQKYFDKGVMLGSLKG"
     gene            complement(13178..14830)
                     /locus_tag="PK733_07430"
     CDS             complement(13178..14830)
                     /locus_tag="PK733_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HOJ10407.1"
                     /translation="MNRQSTFSTIRKSTLAFEEAKITALYCRLSRDDELAGDSNSIVN
                     QKAILKKYAEDNGFRNIEFYVDDGVSGTTFDRPDFNRMIADVESGRIGTIIIKDMSRF
                     GRDYLKVGYYTEIMFPEADVRFIAINNGIDSANQADSDFTPFLNIINEWYAKDTSKKI
                     RAVFKSKGQSGKPLCTNPPYGYIKDPEDKLHWIIDEKAAEVVRDIFRLCMAGFGPTQI
                     AKQLEKRCIDTPTVHLRKMGINTPARPPENPYAWSARTVADILAKMEYLGHTVNFKTS
                     KKSYKSKVKILNNPEDWLVFKNTHEAIIDEGTWETVQKIRDGKRRPSRLGEMGMLSGM
                     MFCADCGAKLYQVRGKGWTHDKEYFVCATYRKKKGMCSSHQIRNVVVEQLLLEDLRRV
                     TSFAKDHEQEFIRIVMNNSEKELAKELRQSQKEYEQAQTRIADIDKIIRKLYEDNVMG
                     KIPEERFYKMSAEYEAEQKALEERISKLKHTIDTANEQSLNTDRFLALVKKYTEITEL
                     DAEIIREFIDKIIVFKAEKVDGRRTQRIQIFYNCIGAIDLPN"
     gene            complement(14888..15091)
                     /locus_tag="PK733_07435"
     CDS             complement(14888..15091)
                     /locus_tag="PK733_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HOJ10408.1"
                     /translation="MENQSNSRTTKSDIGGTVYVVESRVSDSAKESAYSKLKRLITVN
                     AKNLSKLSDSSNKSTEINSTSSR"
     gene            complement(15167..16066)
                     /locus_tag="PK733_07440"
     CDS             complement(15167..16066)
                     /locus_tag="PK733_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOJ10409.1"
                     /translation="MATSTTYNRAFWNVMKGKEENNQNLSEGFDNAGAYVAPDEFREG
                     FNTALAKENVFRRFATVINLSSAEGKIQAVSSTGTADWVEDGDPIPESADTFTQFLVK
                     SYKLASLVKLNRSFVTDMNFNLEKYLMGDFAKRFGKAEENALFNGNGTTQPTGILTAD
                     ADVATADSVTISFDEITSLYFSLKDEYRNNAVFIMHDNTAMLLRTLKDTSGSYLWNSS
                     DNTIFGKPVVTSPYMPTVSAGAKSLVFGDLSYYWLIERQPITIKKLSELYALQGQIGF
                     SAYERLDGKLIQPDALKILQIKA"
     gene            complement(16331..18634)
                     /locus_tag="PK733_07445"
     CDS             complement(16331..18634)
                     /locus_tag="PK733_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOJ10410.1"
                     /translation="MPQKLKDKPQFCCWKYEERSGRKTKVPYNPVTGKRAKPNQRGTF
                     KDFSSAVAAISDYDGIGFLVGNDICVVDLDDCFVSSGKLKPVAQNVVEAFSGCYMEHS
                     PSGKGLHIFFKATGFNFDKTKYYINNRKLGVEVYVAGAINRFVTVTGNMFADGDIAEK
                     SYELQMILDKYMLRPTPVKQILDAESQSYLSDKSVIEKALKSANRERFKALWQGDTSG
                     YASASEADLALCGMLAFWCGRDVGQMDRLFRQSGLMRDKWNRPQSGSTYGMITIEKAI
                     ANATEIYKPGGKRSSATEDFGECSLTGFKPESNDRYPWTDIGASRLFADYYKSFARFV
                     PERKMWFCYENGIWIPDVGNLKVMEMCKSLANQLLTYALTIQDEHQRKAYIDYCRKWQ
                     SRRYRETVLKDAQSVYPISMAEFDQDPLMLNCANGTLFLMSMDFRPHNSEDRLTKISG
                     VKYDPEAKSERWNRFIHEIMSGDEEKAKFLQKAFGYSISGDTRYECLFVLYGATTRNG
                     KGTLCESVLKVLGSYGCTARPETISLKKNNNSSSPSEDIARLAGVRFVNISEPSRGLV
                     LNAAQVKSMTGGDTINARFLHENSFDFSPKFKLYINTNYLPVITDMTLFSSGRVVIIP
                     FERHFDESEQDKNLKREFAKAKNQSAILNWLIEGYQLLKKEGLTLPNSVKTATEAYKR
                     DSDKIALFFEDALEESPNSEVRTSEVYARYQRWCSANGCYSENARNFKQALTAIARVE
                     RKRPRSGGGMTTMLIGYKLTEEEFLLI"
BASE COUNT         5552 a         4039 c         3076 g         6589 t
ORIGIN      
        1 cataatagat tttaggttcc tctaaaaatg aggttggata gatgactacg ctatacagta
       61 agccataatc ttcaaggtat ttgattaaat ctctgcactc ctgaggagca gcaatcttca
      121 aggaatcaat ctgagaaatc attttaataa gatcattttt ttcaatttga ttttttaaat
      181 aaaaatattc ggacagcatt aatagagatt tctttactat attatcatta gcgtcatatc
      241 cgtagttaaa tatagatcta aattcagagt caattttctc tattttttta ttaagaaggt
      301 ttgtaattgt aattgatgat tgttctattg cttccacttc tttgtgatta tataactcac
      361 agaaaatttt taatgctagc ggcgttttta tgctccatct cagccattca tttccattaa
      421 gtgttacgtc atatgctttt atatatgcat caaacaaact acttacaggt gcatccccat
      481 ctgatgttga tcttagcaca ctagacatta ttcttttatc tggtgaagtt acattaaata
      541 catatggcct actcgtaaaa cagaacttaa cccttggata ctttttacat atagcttcag
      601 tttcaccaat taagttaaac catgtgtcat agggcctaag ctcatctatt ccatctacac
      661 aaattaaaac tttaggcata atatatattc catcaactgg attgtttttt tccaatacct
      721 gcctatgttc attcctgcaa gacaaagaag atagcccctg ccaaatttca ccttcactcc
      781 aagcatttga taaaccaagt gctcttgtca atatttcatg ccaagttgct ttatttgata
      841 tagattttgc tcgtatcaag attgatacat gatttttact catattaaaa tgctttacta
      901 cattagcaat accatgtgtt ttccctgttc caggatctcc taaaactaat aaggtatctt
      961 cttttagttg actaatggtt gtaatgatat tttcatcagc actactatca atgattttat
     1021 gtatgctttt cttgatttca gagaaatgga ttggtttttt attctttgaa catttttcta
     1081 gacaccctaa taggtcgtca aaatcataag aagataataa ctcttcaata tgtgtgcaaa
     1141 ttagtttatc cgaacataaa gattctataa aaacatctat atgactaata gtccttttaa
     1201 ctttaatgct tacctgattt gctatttctg taaggtcttt tttattaaat aatgatacac
     1261 agtcatctaa ttctagcaaa aaattattac ataaatattt gagttctttt aattttacac
     1321 aaactaaatt cctttggtta tggcttccta tcaacaaatt aacgtgttgg tttatagcac
     1381 catttacatg taagtctggt aaatatcttt ggcttaacca actgcttttc tgtttttcaa
     1441 aagacaaatc aattatatcc tgtgagatct ctgatttttc aaaccaatat ctcaaaattc
     1501 ctgagcattc agggtattgt aattcagtta atattctagt actgttccat aactcaattt
     1561 ccagattggg caaagaaatt agcaattctt tttttaattc ttcccatctg ctagattctg
     1621 ttttttcgct tattttatta cctttactca tcttagaaga agatagatcc cttggcacac
     1681 aaataatata cttttttatc aatggtctaa tttttatagc agttttaact gaattttcaa
     1741 tttgcctgaa ttgactatct tcaatactgc ttttaaacca cttagattgt acagcaacta
     1801 tactgtcatc tttcgtgaca gtgtacgctt ctactccacc atccccgcca gcgccattca
     1861 caaagaaaaa ggattttata ttattagcat agtttcttct acaccacaat tcaaataatt
     1921 gattgcacat agtttcaaac gcatgtgttg gtgcatcatt ataggtttga aaatgatccc
     1981 aattcataat atcaccccgt tggtataagc ccttaactat agcctgcgat aactcataat
     2041 tctatcttaa tctaacatta ttattcaatt ctatatttct gccgcccgga agaagtcttc
     2101 tcaatcgctt gtatagaagc ttttgacgta ttgatataat taaaaaataa agtaccaagc
     2161 aaaagacgtt tgcttcggga aatgcgattc cattcataga ctttaaataa atctctcact
     2221 aggaaaactt ctgattaatt cgaattctcc gtttctttaa tcacaatatt tagaagttca
     2281 cttacattcc gcataaaaac cctccgcaca atgttgatgt caacaatgtt attgatatga
     2341 ttatatcgca aatatgtaat tatttccatt agtttaacaa tattctatat aaaatgagaa
     2401 tgccagctat aaaccgacat tctctcataa ttgcctagcc tcaaatttgc cttacactgc
     2461 acagcataga catgcctaaa cactctactc aatccactga gtaaacatgc ctacacactc
     2521 aaggtctagc actacggaaa cttcaatttt atttttcaaa accttatttc tgaaatctcc
     2581 gccatccgtt gtgaatgtag tgaatttcgc aaagtgctga aaactcagga cttctaagta
     2641 tctttccttt gcattcctat tagacactct acatggcttg ttgctgcaat tatgatggcc
     2701 gtcgagcctg gtaccccctt ggcgtcaggg tacatttccg aaatcttgct cctttgcaaa
     2761 ctgtttttgc gattcaatac cctctttcac acttgcaatc caggtaaatt gtaaataata
     2821 aaatctcttt aaaagcatga tattccttcg aatgctttat tcggtgcaat ttcactcggc
     2881 gttattccta tttttgtgct cagtcttgtt ccccgactta ataatacact ataattttca
     2941 tcttcaagaa tacgctcttt aagccttata ctaaaatcat cggtaaccga atatctttat
     3001 tctgtttacc aaagtgttcc tcaattaatt ggtttgttac agcatatccc tgctcacaat
     3061 ggaagtattt cataatcaat tcttcgatta ccctcgtgaa cttgattata attatccaat
     3121 gctgactgta acgcagttac atctgaagct tttttcaagt gaatatacaa atctaccttt
     3181 gcgcgactac atgcaacata gtacattctt gtaaattcct ctgcgttctc ctcattaaga
     3241 agttgcgggt ttgaaaacat actggttaac gtagtattat ctctatccct acggctattg
     3301 ggttcaagac tgacaataac cttttcccat tcacgtcctt tcgcttgatg gacggtcata
     3361 tagcggctat cctcagaaaa aacttctgta aagagcttat gcgatgtgta ccaactgagc
     3421 aaagaaacat tagttttaat ttcatctcta tcttcatcgt cgaaaatcgg aatggtaaat
     3481 tgttcctctc caataaccga tacaagttcg gtatactttt cttcgtttag ttgagaactg
     3541 aatccattga ttatatcaac ggtcaaagct tctacagaaa gatccccaaa cacttcatct
     3601 gtcaaccgct ttaattgcgt aaggattttt ggtgttaatt tcttcgtgtc aaagtctgtt
     3661 atgagtgcaa gtgcagcgat tatttcaaca agcgcaccag ttttataccc attccagagt
     3721 ctgaaaataa acttgaaagc ccgaacccaa gttacatagt tatgttcgac aatatcgctt
     3781 cgtatatcaa tcggcgaagt ataataggca ttataaattg cttttaaact tctctcttgc
     3841 tctccgttta tgtcctggat ataggaaaat gctgctgccc aagtgcgggt aagcactaca
     3901 gcaccatcag caatcgcgga gtttatcagt tgcttggcag ccgcatcttc accagaaatg
     3961 atatggattt ttgtcttctc tgccgattgc ttttcctcat cactgttata gcacttttcg
     4021 ctattctgag tgacagttga atccgctttg cgcagaaaat tacatagatt aactacatta
     4081 gcggttgatc gtctgttgcc gctaattgcg tattcgcttg aagcccgatt gccatctata
     4141 tttagggcta aaaactgcga tggccttgcc ccttgaaagc tatatataga ctgtgcaaca
     4201 tcaccaatcg ttccaataac ggttgacttg cttcctatta acttgattag caaagtttgc
     4261 agagggtttg tatcctgaaa ctcatcaaca aaaataaatg ggaacttgac tcttgttgca
     4321 taaagcgcag tatggttgtg ctgaagtata cgatagccaa agtaaaggat ttcattatga
     4381 gtcagctttc ttaccttcga ccaagtatat tgctttatag gaatgatgtg attattgtta
     4441 atctgtcttg atgcccgtaa ttttgctgtt ttgtcagcaa ggtatgccgc aagatcgacc
     4501 tcaacttcac ctaatatttt cttactgtaa gacaattctt cggtatcccc gaccgccgat
     4561 tttatgaact caaatatatc tgttttgtcg ataccatgca ggattcccat tccctcgact
     4621 tgtgacgtaa tcttactttt tccttttacg gtaatgccaa agtcctcccg cataatttgc
     4681 cgcaaatctt cttggaacgg cttgatgata tgctcaataa taaacccatg aattgtatgg
     4741 gcttcaacag atgaagaaaa cctatcaaga cgatgcttaa tttcatcaac cgctgcattt
     4801 gtgtaggtaa tacaaagaac cttcctctct ctgctttgca caacgagggg ataggtagta
     4861 acaatattct tcacgttttc cacgaggaaa tgtgtttttc ctgcacccgg cccggcatat
     4921 atcttgatat gactatcaag ctgttctggt gttagtagat cacttgaatt tattccgata
     4981 gacatagcca tttcaacccc tcctgtatgt aagcgggaac agttaagtcc ttcgccaatc
     5041 cctcatccac aagtatatta agagcaacat cgcctttaca ccctttagca taatgtaaaa
     5101 acaagttggc aaaaaacaag ttctcatact cagtcttgtg agcagtatcg tcctgaatcg
     5161 cttgcttata tgcatccagt cgtttcttta caagaggttg tgagcgtcca tctaattcgg
     5221 gtaggtgtgt ttcccacttt gcaaagtcaa acccatgttc atcaacaaag tcagatataa
     5281 cctgacttgt tggcatctta aaaattttta atgaaatctc cggattatca aagttgctaa
     5341 gtagcaactc atcctcaaat gtgcgcccac caatcgactg tgttgatata tgaaaattat
     5401 caatcggaaa gcggttttta agtttctgta tatgttcagg cgcggtcgtc tgatatgcct
     5461 caattttttc tgttttgccg tctgcaatcc acttaaaatc gcagtcagtt atacacagaa
     5521 cttttttatt aacagcgttt ccattaaaca actccacaaa atgctcgaaa tgcttaccac
     5581 caatttcaac cacggcgata tgctcatcct cgtaagaaca gccacacttc tccataaaca
     5641 agggcattaa aagcctctct gcgatgcctt caaccagtat cactttatcg gcaaacaaca
     5701 tatctgaacg tgtgacatcg agaaatttcg ttaagtgctt tttagctttc tctttatgct
     5761 tttcggcacc ctcactgtca gtaaactgca cagaaagact ctgttcttta caatcggaac
     5821 aatcgccgtt cctatcatag gcaatcataa acatattatc tatgcccgct acggctgaaa
     5881 tgttacttga atgtgtagta acaaatatct gctgattcaa gttgtcctct tgatctaact
     5941 gacgcaaata tttgaacagc ttgtactgca aagcgggatg taaatgtgct tcgggttctt
     6001 caaggagtag aattcggaag tccctgcccg gacggatttc cgtcagtttt atcaacatat
     6061 aaatattaat caggttgttg taacccaatc cgttataaga aagcggaaca atataatcag
     6121 tcttggtgtc ccttacttct gtgatatatg aatcccccac ggatacattc ggacgaagat
     6181 tttgtgaaat cgacacattg ccgtgcgaca gcccaatatc attgttttcg ccctcaaata
     6241 acttctctaa cttatctaat acagattgaa gaatggcttt catgtcgagg gctacagaat
     6301 cccgcaactc tgcaatcgca gtagcattcc cctgatcttt cgcaaaggca tcaatttcac
     6361 gcttagtctc cttgtggaca tcatcactgg ttcgttccgc accaataaag cgaatatcaa
     6421 atatttcggt agcagccttc ttgtcagcag cggtattact ggccgcgttg taataagacc
     6481 acgaatagcg attctcaaat gattttagaa tgatcatata ttttccaaaa tcattatcag
     6541 ctactttagc aacttccctc ttataatcgc tgagagcctt ttcatcaagg gtgcaaacag
     6601 ctttgatgat agcggtaata ttgtaattgg ttgccccatc agcttcctgt tgaaattcag
     6661 taagttcgtt catgcccaag aatggcaaca atctgataat gctttcatcc tctgtgtctt
     6721 cttccgaaat agtatgacaa atggtatact caatcgcaat ctgcggagcg tcctcacggt
     6781 attttgttcc aaatccgagc agttcatttt tattgaaatc atcagcacaa acataaggct
     6841 cacgcaacaa tcttacagca gtaagtagcc ctgttttgcc agagttgtta gcgccgatta
     6901 taacattaag tcccttatga aaattcatgg taaaatcctt ataatttcga tagttcttta
     6961 ttgaaattct ctgtatgtac attagctgac ctccgctcta ttttattatt tataagttac
     7021 ttgctgatta tctacgctgc ttgacgctta acaaattaaa ttccattgta gtaataccaa
     7081 ttatcttact tactccccac acctctatgc tacctttagc acttatatta tagattaata
     7141 attaatatac tagatttttg tgtttacaac aaattgatga tttaaatatc agtcacctat
     7201 gcttttatta tttgcagttt cgaagtagct ctattaatta ctcgcaacac gaatattgcc
     7261 tgaaaaattc ccctttttca tttttcttat tttttttcaa gctattaatc aaattcatat
     7321 tttaggtacc tctactggta cctctgcact atattacaca tttcatcata tttacaaatc
     7381 atatcctgag ccagtttaat ctggcatctt gccctatcca aattatgatt ttctctatgt
     7441 atacggaaat atttgtcccc atttatatgg tcagccagga agcgtgatgc cagttctatt
     7501 gttatgatta tagcttccag tgccatgtat ctagttcttc ttttatcagg aattcttttg
     7561 atgctatcat gaatcctttt gtaaattctt catatttttt catatctaac tcaacttttg
     7621 ataagtctgt ttcaacttcc attgcagtgt tagcagcaaa gtggatagta tcaccaaata
     7681 agttcaaaat caaataaatt atcacctgcc ataacaagaa tatcatcttc tattttctca
     7741 ttatctatag caaacttaat atctgctatt gcccccagcc tgttgtcatt tgaagtggta
     7801 aggtcatttg tactttaatt actttagaac tcttataccc tttaacccac tctacaaact
     7861 gtgagtaaaa cctatcattt gatactatgt atataaaatc aacaaacatt aaatgttcat
     7921 cagctctcag aatattagct tactgatcaa gggtatcatt atccaatcct ataaaatatg
     7981 tttagtttct aatacaaaac acaacttcat cgttctagac ctctttaagc actcacttct
     8041 ttctcttctg ttgcaaaaaa ccactgatac caaacttatc acaagaatat attatctgtt
     8101 tgatttatcc tacactcaac ctatagcttc gccttttcgt gtaaaatttt ccctcacttt
     8161 acactacctg aatttttgta tgcagtccta aaagatttga cacgctcgag tcgatgatca
     8221 attttgatag gactttgatg gtatggttaa attaaaaata aattctattt gcccatattt
     8281 tgttaaagta agctttgtaa tatattatcc aaaatgccat caaataaagt tccctattta
     8341 tgttaatact atagtgacat tatattgcat gtgatttgta ttatgctgtc gaattttgtt
     8401 gaattgttat ttcgtatata cgaaaatatg caagaatgtt ttttattaga taccatcatg
     8461 ttctggcacc gaacctatcc cccggctaca tttattatat atgttgtaat agcttttgta
     8521 ttatttgtag taaattgtaa cgtatgtata aaaaatatat gcaatccatc aagaatttgc
     8581 atatattaaa ccattatctt tttcgcttgt cgtatctgtt gtatttggtg ccgggctaaa
     8641 aatacaacgc acatacacca ggatttacag taacctttgt catattaata atcaccttag
     8701 ccattatatt taaatttata ctctttgcgg ctgtttattg cctgccactc tgtatatatc
     8761 ataccatttc atatgaactt gctactagta cctctgcact aaattacaca tttcatcata
     8821 tttattcatc atattttgag caagtttgat ctggcatctt gccttattgc aaaaaacctt
     8881 gtccctttca atatgtaaaa agtgcctggc atgtgtgcat ttcgtgcctg gcaccgagtt
     8941 atttcttttt aagccttttt atgaatcctt caagtcatat tatctgtctg taactttttc
     9001 tattattgtt ctttaggagt tttgtcaaaa taaatagcaa cacataaaaa tacaaaatct
     9061 tcatcggata atcccgtaga ctgatggata gcattcctcg ggatatatat tactgtgtct
     9121 ctccctactt ctttttcaat atcaccaact tttaccctac ctttaccgct taatattatg
     9181 tatacttctt cctcattatg atgagagctc tcatctgttg taacaccttt ttttactgtg
     9241 actatattag attctataat ctccgcttca tcgccagaaa ttatatatcc taatccttta
     9301 tccttgcatt cagaaacttt cctaaatata ggttttatca taataaactc cccttcttat
     9361 tacattgtta ttttagaaat atgagataag tatttttact gcaactataa ttgatttaaa
     9421 gcattcctaa acccttccag aaaataatta tcaataccct tcttgaattt gcctttcatc
     9481 ctatactccg ttacggatcc atcaccagct tcatacccac cttcttcaag cattttatca
     9541 ctgggaacat aagctattgc atcattatat ccaaaataca atacatttgc ttcaggaagc
     9601 agctcttgca aaaccttctt tacatcatag gaaggctcgc caccgatagt aaatatatag
     9661 tgccctttcc ctatacggat aattccacaa tttagtatta aataatcatg caatctatca
     9721 tagttattta caacatattc tgcattggac cacattaaca atgaaccatt gctttcataa
     9781 tcctttacca tattcttgtc aaaaggaact ttagaattat cactgaaatt ctgtaattcc
     9841 ttttcaaaaa aggataatgg gtaaacttct aaaggtatct ttatttgaaa cattcttgac
     9901 gataatgatg gttgaattac ttcccacttc ttgtttatta gtgagctttg aatttttaac
     9961 gccattgaag aagcaacttc attacaatct tcatatgaaa taggatggaa ttttgatgat
    10021 ttcgcaccta tctttaattt tgagtcacca ccgaacccct ggaaaaatac agcagtacac
    10081 ccataattat tagcctcaat taaatgacat agacggccag gatattcgct tgacaagatt
    10141 ctataactat ctagattaga tggatggcat gcaaaattta tagcaattac tttaacattt
    10201 ccacttttat cagtcaattt taaaagatac atattcttat cgcattctcc atcaggattt
    10261 ggcaatatag ctctttgcat aacatcatca acttttagcc tacggcttat attccattcc
    10321 cccaatatac ttgtatactc tattgttcct tcataaatgt ttaagaaagc ccggtttata
    10381 caatccttta ttctttgtag gaggaaactt tcgtattccg gagaatggaa tataaaatcg
    10441 taccctttta aacaagggcc aaagtgcgta tgcgtatatg aaattagtag attattatag
    10501 ggtatctgat acttttccga aaggtattct cctattgata taggtaggct atcatcatgg
    10561 aagcataagt caagtgaaag caaaactaag atttgttttc catcttttaa taataagcat
    10621 ttaacataaa ggtcatcatg gataccggta aagctcttgc cgaaaaccgt cccaaaacct
    10681 agcatcgttg tttccacgca gggagttaca atatcctttg caacgccgaa cataatattc
    10741 tttgacatag aaaccttcct tcccatataa tattagctga gggttagggt atgcctaccc
    10801 tcagcactat attcagctaa cattattcca ttattcattc cacctgtcat aagccttttg
    10861 ttttatttct agaatttcgt caattcccat ctctttaact tttgcaacat atgcatccca
    10921 tttactcagg ggttctattc cggtaataaa ttgtgccctg gcctgcgcaa caaaattatt
    10981 aatatctgtt aatttattag ctataacgtt attttcttcc tcggtgactt ttatcaaagt
    11041 atcaggaaat gaaggctttg catatggtaa attattttta agcatacctg tttctcttgc
    11101 tatattatta gctcctccag tagttgcata agtaacccaa gcactataaa actggttcca
    11161 ggatgcatat ttggtaatcc ataaccatac gttcgagtcc caatcgtctg gttgcaggaa
    11221 actaatgtgt gatttttcat tgtttgtata tttccagtta actccttcca gtccgatatt
    11281 ctgcgagagg caatttaacc ctgaacctgg gacaacttca tcttcagtat aattcatatc
    11341 aaacattctg agaagttccc ggacatactt gcaattcttg ttaattgctg ctgcggaaga
    11401 tgatacccca atataaccct tgacctttct ttcacttgtg tattgtgatg ttaacggtgc
    11461 caagcaatct atattgattt ttccatctgg gaaatctgat tctgcaagca tatcagcact
    11521 tgtcataaac gccgcttgcc ctgcttttat cctgccactt gaagtatctg aatcaattgt
    11581 aaataattca ttttccagca atccttcctt gtataacttg ttcgcatatt ccaaatatct
    11641 tcgatattgt tccgaaatcc tattgtatac aacttttccc ttaccatcat ctgcgaaatc
    11701 ataatcatgg gcatcaccaa acgctgaaaa tagaaacgct tcagtatggt attcgaacgt
    11761 tcctttattc aaaggaagaa acggtgcata tcctcctgtc aagccggaat cctttattgc
    11821 cttaagtacg ttgtaaaaat cgtctacagt ctttggtatt tctaagttta atttctttaa
    11881 atagtcagta cggtaaaaca tttgccctat tgaataagtt gaatgtacct gaattttagg
    11941 gagggtataa atagcaccat ttagttgccg tataactttt tcagcttcag gatatttttt
    12001 aaaccaggat acaagatttg gcatatactc atagattaag tcagaataat cataaaacat
    12061 tccgccttct acgccataaa tgaattgtcg ttcgttatca acataagcat ggaagaagtc
    12121 agggaaatcg cctgctgaaa aaaataattg aatagctgtt ggctctgaac tggaaatcat
    12181 attccattta atttgaatat tgctgatttc ctctagcttt tgccagtaag acatttcttt
    12241 cgggtctcca tcatatgttc ccggtgcaaa cattgatgtt actgtgatgg gctttttaac
    12301 aataggataa ccaatcgcat taaaattttc atcagttttt gagatatctg tctcctgccc
    12361 tgcagttgtt ttttggttat tttttgttgg tgcgttacaa ccaagtaaca aagacaacaa
    12421 aaaaataatc aaagtaaaca tggctattga tctttttctc attttaacat ctcctttttt
    12481 tattatatat tatataaaac agtaaaaact gttgtataaa ccctttgaaa gtgttcgtac
    12541 ttaccctttc aaagatccga gcataactcc tttatcaaaa tacttctgca aaaaggggta
    12601 taggattaaa acaggcaaag atgatacaat aataacagag tattttaaaa gatgctttaa
    12661 ctgttccata aggtaaagcc tttcaccacc aattttttct gccatagccg cactcatttg
    12721 ctctgttatt aatatttctc tgagaaataa ctgaagaggt ttctttgctt tgtcagaaat
    12781 atatataagc gctgtaaagt aagaattcca atggccaacc gcataataaa gggtcataac
    12841 tccgattaat gcctttgaaa gtgggattat tatctgtaca aaggttcttg ctgttgaaca
    12901 tccatctata tatgcagctt cttcaaggtc cctcggtata ttttggaaga aagtgcaaca
    12961 tataatcata ttataaacac taattgcccc tgttataatc aatataagtc tcgtatccaa
    13021 taacccaagc tttttaatta ataaatacga aggtattaat ccgccagaaa agtacatcgt
    13081 aaataaaaag taccccatta ggagcggtca gatagggatt tataattcct gaaaaattcg
    13141 gcatagtcgt agatttgcgg ctatgccgtt ttttcgttta gtttggtaag tcgatagcgc
    13201 caatgcagtt ataaaaaatt tgaatccgct gggttctgcg gccatctact ttttcagcct
    13261 taaatacaat aattttgtca atgaactctc ggataatctc tgcatccaat tctgtaattt
    13321 ctgtgtactt tttaaccagt gccaaaaagc ggtcggtatt gagggactgt tcatttgctg
    13381 tatcaatggt atgctttaat ttggatatcc tttcttccag tgccttctgc tcggcttcat
    13441 attcagccga catcttgtaa aaacgctctt cgggaatttt gcccatcaca ttgtcctcat
    13501 atagctttcg aatgattttg tctatgtcag caatgcgagt ctgtgcttgt tcatactcct
    13561 tttgactttg gcggagttct ttggcaagtt ccttttctga attattcata actatacgga
    13621 tgaattcctg ttcatggtct ttggcaaagg aggtcacacg ccttaggtct tctaacaaaa
    13681 gctgttccac tacaacattg cgtatctgat gtgaactgca catacccttt ttcttgcggt
    13741 aagtagcaca aacgaagtat tccttatcgt gtgtccatcc cttgcctcta acctgataca
    13801 gctttgctcc acagtcggca caaaacatca tgccagaaag cattcccatt tcacctaatc
    13861 gtgatggtct tcgcttgcca tctctgattt tctgcactgt ttcccaagtc ccctcatcaa
    13921 taattgcttc atgggtattt ttgaaaacca gccaatcttc tggattgttc aatatcttga
    13981 ctttgctctt gtaggacttt ttagaagtct tgaaattcac tgtgtgacct agatattcca
    14041 ttttagccag aatatctgct acggtacgag ccgaccaagc atatgggttt tcaggtggtc
    14101 ttgctggagt gttaataccc attttgcgaa gatgaaccgt aggtgtatca atgcatcgtt
    14161 tttcaagttg ctttgctatc tgcgtgggtc caaagccagc catgcacagt cggaaaatat
    14221 ctctgaccac ttcggcggct ttctcatcta ttatccagtg caacttatct tcagggtctt
    14281 taatataacc gtaaggtgga ttggtacaga gtggcttacc agattgtccc ttggatttga
    14341 acacagcacg gattttcttg ctagtatcct tagcgtacca ttcattaata atgttaagaa
    14401 acggtgtaaa gtcactgtct gcttggtttg cactatcaat accgttgtta atagcaatga
    14461 atcgtacatc tgcttcagga aacataatct cggtataata tcctacttta aggtaatccc
    14521 tgccgaaacg ggacatatcc ttgatgataa tcgttccgat tctaccggac tctacatcag
    14581 caatcatgcg gttaaagtct ggtctatcaa aagttgtacc gctgacccca tcatccacat
    14641 aaaattcgat gttgcgaaaa ccgttgtcct cagcatattt ttttagaatt gccttctggt
    14701 ttactatgct gttgctgtct cctgcaagct catcatcacg ggaaagcctg cagtaaagag
    14761 cagtaatttt cgcttcttca aatgctaatg ttgatttacg tatagtgcta aatgttgact
    14821 gtctattcat ttttctcctc catttccgac agtcttcaag cggtttagca ctatgtatat
    14881 taccgtacta ccttgaagaa gtcgagttga tttccgtgga tttatttgaa ctatctgata
    14941 actttgaaag gttttttgcg ttgactgtaa tcagtcgttt cagcttggaa tatgcacttt
    15001 cctttgctga atcacttact cgtgattcca ccacatagac cgtacctccg atatcggatt
    15061 tggttgtgcg gctgttactt tgattttcca tatcgtgacc tcctgtccga tgttttgatg
    15121 gaactggcac tgagccgaat tgatgactca gtgcctaatc cattatttaa gcttttattt
    15181 gtaatatttt cagagcatct ggttgaatta gcttgccatc caatctctcg taagcagaaa
    15241 agccaatttg cccctgcaat gcatataact cacttaattt ttttatcgtt attggttgac
    15301 gttcaatcag ccagtagtat gataaatctc caaatacaag gctttttgct cctgctgata
    15361 ctgtaggcat atatggagag gtaactacag gctttccgaa gatggtgtta tctgaagaat
    15421 tccacagata actgccgctt gtatctttaa gggttctaag aagcatggct gtattatcgt
    15481 gcatgataaa cacagcgtta tttcggtatt catcttttaa tgaaaaatac agagaagtaa
    15541 tctcatcaaa agaaatggta acactgtctg ctgtagctac atctgcgtct gccgtaagta
    15601 tacctgttgg ctgtgttgtg ccatttccat taaataatgc attttcctca gccttgccaa
    15661 aacgcttcgc aaaatcaccc atcagatatt tttcaagatt aaagttcata tcggtgacga
    15721 atgagcggtt tagcttgaca agagatgcca gcttgtatga tttcaccaga aactgtgtaa
    15781 atgtatcggc actttcgggg attggatctc cgtcttcaac ccaatctgct gtacccgttg
    15841 aggataccgc ttgaatttta ccttctgcag aagatagatt gataacagta gcaaatctac
    15901 ggaatacatt ctcctttgcc aaagcagtgt taaagccttc tctgaactcg tctggtgcga
    15961 cataggctcc tgcattatca aagccctcgc ttagattttg attattttct tcttttcctt
    16021 tcataacatt ccaaaacgct ctattataag ttgttgatgt tgccataatc gtttacctcc
    16081 taaattttat taaatgtggg gtatataccc cgtttgaaac cgcgtttttt tacgcgttgc
    16141 cccacgcccg ttatccagat aaaaaggtgt ggagatttga cctgccccta ggggcaggta
    16201 tcaacacatg gagtcatatc cagtggttta aagctttgta gcaagatgta gcaggtaaaa
    16261 actaaactct ctatacgaga gcgatttttt atataacctg tttttcttgc tacaagctgc
    16321 tacactgtgt ttaaataaga agaaattctt cttcagtcag cttatatccg ataagcattg
    16381 tggtcattcc accaccagaa cgtggtcgtt tccgttctac acgggcgata gctgttaatg
    16441 cttgtttgaa gtttcttgca ttctccgaat aacatccatt ggcactgcac caacgctgat
    16501 accgggcata cacttcggat gtccgcacct cactgttagg gctttcctcc aaggcatcct
    16561 cgaaaaataa tgctatttta tcgctgtcac gcttataagc ctctgttgct gtcttaacgg
    16621 aattaggtaa agtcaaacct tccttcttta acagctgata gccttcaatt agccagttaa
    16681 ggatagcact ctgattcttc gctttggcga attcgcgttt taagttttta tcctgctcgc
    16741 tttcatcaaa gtgtcgttca aaagggataa tcaccactct gccactggaa aacagcgtca
    16801 tatccgtaat aacgggcaga taattggtgt tgatatacag cttaaacttt ggcgaaaagt
    16861 caaaagaatt ctcatgtaga aaccttgcat tgatggtgtc accacccgtc atgcttttta
    16921 cctgtgcagc atttaggaca agtcctctgc taggttcgga gatatttaca aagcgtactc
    16981 ctgcaagccg ggcaatatct tcacttggac ttgaactgtt attgttcttt ttcagactga
    17041 tagtctctgg ccttgcagta cagccataac tgcccaatac cttaagaacg ctctcgcata
    17101 gtgtaccttt accgtttcga gttgtagcac catagagaac aaacaggcat tcataccgag
    17161 tgtctccgct gatactgtag ccaaaggctt tttggaggaa ttttgccttt tcctcatctc
    17221 cgctcataat ctcatgaatg aatctattcc atcgctcgct ttttgcttct ggatcatact
    17281 taacaccaga tatctttgtg agtctgtcct cgctgttgtg gggacgaaaa tccatagaca
    17341 ttaaaaacaa ggttccattg gcacaattga gcataagcgg gtcttggtca aattcagcca
    17401 ttgatatggg atatacgctc tgtgcatcct taagtaccgt ttccctgtat cttcttgact
    17461 gccactttcg gcaatagtca atgtatgcct ttctttgatg ttcatcctga atagtcaaag
    17521 cataggttag cagttgatta gccaatgatt tacacatttc cattactttt agattaccga
    17581 catcaggaat ccaaatgcca ttctcatagc aaaaccacat cttcctttcg ggaacaaagc
    17641 gggcaaaaga tttataataa tcagcaaaca gcctgcttgc cccaatatcc gtccaagggt
    17701 agcgatcgtt actctcaggc ttaaagccag taagagaaca ttcaccaaaa tcttctgtag
    17761 ctgatgaacg cttaccacct ggtttatata tttcagtagc atttgcaatt gctttttcta
    17821 tggttatcat tccataagta ctgccagact gtggtctatt ccatttatcc cgcattaggc
    17881 cgctctgccg aaaaagtctg tccatctgtc caacatctct gccacaccaa aatgccagca
    17941 taccgcaaag tgccaaatca gcttcactgg cagaagcata accagatgta tctccctgcc
    18001 ataatgcttt gaacctttct ctgtttgccg attttaaagc cttctcaata acagacttgt
    18061 cagacaaata ggattgactt tctgcatcca gaatttgctt cacaggggta ggacgcagca
    18121 tatacttgtc tagtatcatc tgtaattcat acgatttctc cgctatatca ccatctgcaa
    18181 acatatttcc cgttacggta acaaagcgat taatagctcc agccacatag acctcaactc
    18241 ccagctttct gttgttgata tagtattttg ttttgtcaaa gttaaagcct gtggccttaa
    18301 agaaaatatg aagccctttc ccagatggac tgtgttccat atagcaacca ctaaaagctt
    18361 ctacaacatt ttgggcaaca ggcttaagtt taccgctgct aacaaagcaa tcatctaagt
    18421 cgacaacaca aatgtcatta cctaccaaaa agccgatacc gtcataatca cttatagcag
    18481 ctaccgccga actaaaatct ttaaatgttc cacgttgatt tggttttgcc ctttttcctg
    18541 ttactgggtt atagggaact ttggtttttc taccacttcg ttcttcatat ttccaacagc
    18601 aaaactgtgg cttatcttta agcttctgtg gcaagttatc atattttgtt ttcagttatt
    18661 tcacctcctt gcctgttaaa aatgacccga aacgctactg ttccagaggg tttgccttgt
    18721 ttgacacaac ctcgttatct ttcaaggaat acgctcggaa ttatcgtcat ggcatattct
    18781 gcttgaagta tctcttgcat ttcggggtat tcagttgtca aggagcagtg agagaaaaac
    18841 tatgttttca ttgggggatt atttttcctc tcaccttata gccttggtag ggcacattag
    18901 ttgaggattt tctaaaaata tttttcttcc tcatccataa gcgaagaatc caattgattc
    18961 gaacccctta aaacaaaaaa acagcctgtc tattttccag aaaagacggc tgtaatgctt
    19021 atgcttttgc tcaaatttga gcgaaagtgg tattaagttt aagaaggctg aatgttataa
    19081 ctttgccttt ggttttacaa atagacttac agacattttt gtctaaaagt atttatttaa
    19141 ataaaaatca aaaataaagg catacacaac aatcattatc tttattaaca ttgtaaaatc
    19201 ccttcacaaa cacagaatta tcatgttatt tttgaagaaa atgtgctata atggaa
//
LOCUS       DAOSDD010000062        18917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1206_length_18917_cov_5.213021, whole genome shotgun sequence.
ACCESSION   DAOSDD010000062 DAOSDD010000000
VERSION     DAOSDD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18917
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_18917_cov_5.213021"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PK733_07450"
     CDS             <1..619
                     /locus_tag="PK733_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681256.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOJ10411.1"
                     /translation="GGPDVDAACYNEYNYTYNGVISPLRDSMELLVIKKAFELGKPML
                     GICRGIQIINVAMGGTLYQDIASQVKNREVIKHSQQAPVWHPTHDIYIEKDSFVWKSF
                     NEDAIDGDAEEKNAAENDATGNKAAGSNVAGNFVVRVNSFHHQAVKDVAPGFRVTSRA
                     VDGIIESIEYSGEAFIVGVQWHPELMWQKNRVYLKLFDNFIGVAK"
     gene            714..929
                     /locus_tag="PK733_07455"
     CDS             714..929
                     /locus_tag="PK733_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOJ10412.1"
                     /translation="MPVKLNIGDIVELRKEHPCGSKQWEITRTGADIRIKCMGCQHQV
                     LIPRPKFEKSLKKIIVSAESSKESSES"
     gene            1152..2381
                     /locus_tag="PK733_07460"
     CDS             1152..2381
                     /locus_tag="PK733_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ10413.1"
                     /translation="MFENNNRYNDYNKYNGRNRNNFSRTFLTSLITSLLVGTLLIVLI
                     WGNVIPIVPKTGIPGPTPGQSPNLGGGAGVPNPQQIGVTLADDAPSVANVVKSAYAGV
                     VGIAVLRADGKSIFDKNTTEKWGIGSGVIVNPNGYILTNHHVAGGKSKRLIVSFADGR
                     NLDGVTLWSDPVLDLAVVKVSAAGLTAIPPGDATTLQIGEPAIAIGNPLGLELQRTVT
                     SGIISALNRTISIETEQGVNYMEDLIQTDASINPGNSGGPLLNSRGQVVGINSIKVAK
                     AEAIGFAIPINVAIPIINKFNDTGTFNEAYMGIFAYDKEAVPFLDQNIKLDNGIYVVE
                     VDKSGPAAGAGIKEGCIIKQVDGVSISTMMELRALIYSKNPGDTINVTHIERSTGETV
                     TVPIKLAAKEKDGLLTR"
     gene            2634..2945
                     /locus_tag="PK733_07465"
     CDS             2634..2945
                     /locus_tag="PK733_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10414.1"
                     /translation="MKNIEKQNKAEKQCKGNDFEVTIKEYLRQGREKLITDLTGTREA
                     IKLIAKDRTKEFMLATDKGLNKEERDYLIEVIVASMYQTFCYGYGIGKIEGKSNNRVF
                     L"
     misc_binding    3205..3511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3649..5289
                     /gene="ilvB"
                     /locus_tag="PK733_07470"
     CDS             3649..5289
                     /gene="ilvB"
                     /locus_tag="PK733_07470"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOJ10415.1"
                     /translation="MKITGAQAMVRILEMEKVSHIFGYPGAAICPFYNVLVDSTIKHI
                     LTRHEQGAAHGASGYARATGKTGVCVATSGPGATNLITGIATAYMDSIPIVAITGQVP
                     TNLIGRDVFQEADITGATAPFCKHNYLVKNARDLPGIMKEAFHIASTGRPGPVLIDVP
                     IDIQNEYIDFSYPASVDIRGYKPTYKGHPIQIKRIADAIRKSKSPVICAGGGVISGDA
                     CSEMMQLVEKCSIPVVTTLMGIGAIPSNHHMYLGMLGSHGSYSANYAINNADLVFIMG
                     ARVGDRAIVATNKIAKQAVIIHVDIDPAEIGKNIDVAIPAVGDVKSILEDLLDIVDKG
                     SVEAWNITIRGVREKQDLKVSNKNNVDEEGTVKPSYLLRMLSDMVDGSNTIVTTEVGQ
                     NQIWAANNMSITKPGKFITSGGMGTMGYGLPAAIGAKVGCPDNVVIAVSGDGSFQMSM
                     QELGTIKQNKLGVKIIIFNNSRLGMVRELQKMQYCGRYSQVFLDENPDFVKLAEAYDI
                     SGERIDKNSEIGSALERLLADDKPYLLECIVDPEEPTL"
     gene            5453..5965
                     /gene="ilvN"
                     /locus_tag="PK733_07475"
     CDS             5453..5965
                     /gene="ilvN"
                     /locus_tag="PK733_07475"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOJ10416.1"
                     /translation="MGKHILSVLVENYAGVLSRVSGLFSRRGFNIDSLAVGITENPEV
                     SRMTIVVDGDDYIVEQVSKQLNKLVNVIRVKKLDSSNSVSRELALIKVNANASTRSEI
                     VQIVEIFRAKIVDVSKSTLTIEASGDADKITALEDMLKQFGIREIVRTGLISIERGNR
                     HIKANNNDEE"
     gene            5971..6966
                     /gene="ilvC"
                     /locus_tag="PK733_07480"
     CDS             5971..6966
                     /gene="ilvC"
                     /locus_tag="PK733_07480"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOJ10417.1"
                     /translation="MAKMYYDSDCDLNLLKGKTIAVIGYGSQGHAHAQNLNESGVNVI
                     VGLTPNSKRRQQVISDGLKVMDTAEAAKAADIIMILTPDEVQAAIYKESIEPNLKDGD
                     VLMFAHGFNIHFNQIVPPDFIDVIMVAPKGPGHTVRSQYKEGKGVPSLIAVYQDASGK
                     AKEYALAYAAGIGAGRAGILQTTFREETETDLFGEQAVLCGGVTELMKAGFETLVNAG
                     YQPESAYFECVHEMKLIVDLINQGGFGYMRYSISDTAEYGDYVTGKRIITEETRKEMK
                     KILEEIQNGSFASRWIAQNKASGRSEFLAMRRKESEHQVEKVGTELRKMMSWLKK"
     gene            7375..8916
                     /locus_tag="PK733_07485"
     CDS             7375..8916
                     /locus_tag="PK733_07485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ10418.1"
                     /translation="MSTQIKIFDTTLRDGEQTPGVNLNVHEKVEIARQLERLGVDAIE
                     AGFAIASPGDFEAISEVAKNIKDATVVSLARALEKDIDRAWEAVQHAKRPCIHTFIAT
                     SDIHLKYKLKMTEEQVLEKAVAMVKYAKKYCERIEFSAEDASRTRVEFLYRIFEEVIK
                     AGATVINIPDTVGYATPEEFGRLIRSVRENVSNIDKVDISVHCHNDLGMAVANSLVAI
                     QNGATQLECTINGLGERAGNAAMEELIMALDTRKDFYGYTHKIDTTQIYRSSKLITSL
                     TGIMVQPNKAIVGANAFAHESGIHQHGILSEKSTYEIMNPESIGLKQNRMVLGKHSGR
                     HAFEEKLEELGYNLSEEQIKNAFQKFKDLADRKKDVSDSDIEALVEEKVAKIPEIFIM
                     ESYQISSGNRLVSTSTVSVRRNGNVSTEAATGEGPVDAAFNAMERAVGISLKLEDYRL
                     RAVTGGKDALGEVTVRVSKNDKLFTGRGVSTDIIEASVKAYLNAINRTLSELGENIIE
                     NKILK"
     gene            9167..10732
                     /gene="cimA"
                     /locus_tag="PK733_07490"
     CDS             9167..10732
                     /gene="cimA"
                     /locus_tag="PK733_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOJ10419.1"
                     /translation="MRQVKIYDTTLRDGAQAQGISFTVEDKLKIAEAVDRLGASYIEA
                     GNPGSNPKDLEFFQRVKGIGLKNAKVVAFGSTRRVNIDVNDDSNVKSLLIADTPAVAI
                     FGKSWDFHVDEVIRTTLDENLRMIFDTIEFFTKKGKEVIFDAEHFFDGYKANSKYAMQ
                     TIEAACNAGAASICLCDTNGGSFPNEIFKITSRVVESLNIPVGIHCHNDNGMAVANSI
                     IAIQAGASQVQGTINGFGERCGNANLCTIIPNLQIKADYQCIPAESMKYLTSVARYVS
                     EIANVIHDEREPYVGGSAFAHKGGMHIDAVFKNPVSFEHINPELVGNKRVILMSEVAG
                     RSTIMKRINEVDSSICKDSPEAKAIINKMKELEHDGYQYEGAESSFELVIRKILGKYT
                     PFFNLNEFKVIVGEPSINSVNSSAMIKIGVGDQAEITAAEGCGPVNALDNALRKALER
                     FYPEIRQMKLTDYKVRVLDSTSATAAKVRVLIESADGKDVWTTIGVSTDIIDASWKAL
                     VDSIEYKLIKDRI"
     gene            10944..11201
                     /locus_tag="PK733_07495"
     CDS             10944..11201
                     /locus_tag="PK733_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10420.1"
                     /translation="MKLAGNLIKGTKIIRRASFEKEDQSISFHDMLEESLLALCKELD
                     IPVPIWMKKNTGEFAMFRRTFFTSEQFTEKVGFDRFEIRME"
     gene            complement(11431..11700)
                     /locus_tag="PK733_07500"
     CDS             complement(11431..11700)
                     /locus_tag="PK733_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOJ10421.1"
                     /translation="MSESYKILYTKQALKDKRIACEAGFSKKIISILETLKNDPFAGY
                     PPYEKLVGDLAGAYSRRINHQHRVIYEVLQKEHIVKIICMWLHYE"
     gene            complement(11693..11935)
                     /locus_tag="PK733_07505"
     CDS             complement(11693..11935)
                     /locus_tag="PK733_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOJ10422.1"
                     /translation="MSIINATTARNNFFKIMEDVIVTNEPVYVTGKAGNIVVISEEDY
                     RSIQETLYLISIPGMREKILRGLNTPLEDLVEDDDE"
     gene            complement(12089..12985)
                     /locus_tag="PK733_07510"
     CDS             complement(12089..12985)
                     /locus_tag="PK733_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOJ10423.1"
                     /translation="MDINFELYKIFYQAAASGSFSEAAARLFISQSAVSQAIKNLESK
                     IGSRLFYRKSRSIKLTPEGQLLYSHIEQAYNFIKTGEDKIIQLKNLELGEIRIGIGDT
                     ICRYYLIPYLQEFNRRYPNIKIRVVNRTSNQIIHVLKNGIIDFGIVTLPVNEKNISVI
                     DFLTVEDIFVASERFSGLNDKIVKLCELSNYPILTLEQESSTRRLLDEFLASNGVKMV
                     PEIELESIELLVEFAKIGLGIAHVLKESAAEAIKSGELFQIKTTEKLPLRKLGIANMK
                     QIPLSTAAKEFIKIMTFNNCHQ"
     gene            13153..17232
                     /locus_tag="PK733_07515"
     CDS             13153..17232
                     /locus_tag="PK733_07515"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOJ10424.1"
                     /translation="MNMNVVNNNKNNVNNVKRIFVEKKKEFNIEGQALYRDFKYNLGI
                     KGLTNVRIVNRYDVEGISDEDYRASRNTIFSEPPVDIVYDEVFSTGENEKVFAVEYLP
                     GQYDQRADSASQCIQILTRGERPVCRTAKLFIIEGDVSDIEYEKIRNYCINPVECREA
                     SLGKPSTLEMVVDAPEDVSILQGFINMTDEELESLIDNMGLAMSFEDLEFCRNYFRDI
                     EKRDPTVTEVRIIDTYWSDHCRHTTFLKEIKDIEIKDGRYTVPIKSAFDSYISSRKFV
                     YENRKKDICLMDIAIIAMKELKKRNLLNDLDESDEINACSIEVKVDVNGRNEDWLVMF
                     KNETHNHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSGDPRTPVEDTLPGK
                     LPQRKITTGAAAGYSSYGNQIGIATGQVVEIYDEDYVAKRMEIGAVIGAAPKNNVLRE
                     KPVPDDIVILLGGRTGRDGCGGATGSSKEHSKESLVNCGAEVQKGNPPTERKIQRLFR
                     NPEVSALIKKCNDFGAGGVMVAIGELADGIEINLDAVPRKYEGLNGTELAISESQERM
                     AVVVSPQNVNKFIAAAAEENLEATPVAVVTEVPELKMRWRGKLIVDIDREFLNSNGAK
                     RETTAVIASPVEDKNFFRIQAGSQERMEDEPLQAKSSKSRDDIQDNIKSTWLNNLGRL
                     NVCSQKGLVERFDSTIGAGTVLMPFGGKYQLSPSEGMVAKIPVLDGDTCTGTVMTFGY
                     NPQLAKWSPFHGAVFAVLEAISKIAAIGGNYKNARLSLQEYFEKLGKNPEKWGQPLSA
                     LLGAYYAQIKLGIPAIGGKDSMSGTFRSVLSDISGDISEITEEMNVPPTLVAFAVSVI
                     DVNHVISSEFKKAGSKVVMLHIDRDINEMPDFEQLDKNYTRIHELIADDKILASAAVK
                     TGGIAETISKMCFGNMIGFKFEGHVEKELLFNPEYGSIILEIPEREDLDILFQGLKYH
                     VLGHTAEKPEINVNVNVSGNGSEIGSEIGSEIGSEIGNGSNNVSVSKVAISLEEALQR
                     WQEPLEGIFPTKVEAKFPTKGSGTLPTKAAGTFPEKLDWNYNLKDNLNYNNCNKIKST
                     RTLKIVRPRIFIPVFPGTNCEYDAKKAFEKAGGFADVMVIRNLTSKDIEESIEMMAKK
                     IDNSQVIMIPGGFSGGDEPDGSGKFIATAFRNPRVKDAVMRLLKERDGLVLGICNGFQ
                     ALIKLGLVPYGEIRDMTDESPTLTFNTIGRHASCMVYTKIVSNLSPWLSNVKQGEMHT
                     IAISHGEGRFVASQSDLALMERNGQIATCYVDPAGNPTYDIRFNPNGSVMAIEGITSP
                     DGRVFGKMGHSERIGKNVVKNVPGEKDQKIFDAGIEYFR"
     gene            17300..17764
                     /locus_tag="PK733_07520"
     CDS             17300..17764
                     /locus_tag="PK733_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOJ10425.1"
                     /translation="MCIYERLKELGIELPKTPAPLGVYFPAKEFGNKFIYISGQGPSV
                     GGKPLCVGKVGKDLSLEEGQKAAYACMLNVLGVLNDKTGDLNRVKSAVKILGFVACAN
                     DFYDQPKVINAASKLLVDVFGEEIGKASRSAVGTNALPGNIPVEIEAIFEIE"
     gene            17902..18603
                     /locus_tag="PK733_07525"
     CDS             17902..18603
                     /locus_tag="PK733_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOJ10426.1"
                     /translation="MSRKIIGVSLCVLILLSIIQIIIDCRSETENSNAVISAARRPEY
                     VIFIEVEDKTLYLLEDGACIKKYPIASGKSGYPSPIGYWKITTKEKWGEGFGGRWMGL
                     NVPWGEYGIHGTNKPGSIGSAASQGCIRMFSDDVKELYDIVPLGTRVVIVNGLFGPFG
                     RGFANIEPGDRGADVMEIQRRLKDLGYFKGWLSGVYEDDLKNAVHRFQKDKGLPVINT
                     ITRECWEAMGFREFE"
     gene            complement(18668..>18917)
                     /locus_tag="PK733_07530"
     CDS             complement(18668..>18917)
                     /locus_tag="PK733_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ10427.1"
                     /translation="ETKNILKWISENLPKTVYISLMSQYTPVYKAENYKELNRHIRKS
                     EYETIIDYFFDIGLENGYVQELSSADTIYTPVFDLKGI"
BASE COUNT         6388 a         2872 c         4285 g         5372 t
ORIGIN      
        1 gggggggccg gacgttgatg ctgcatgtta taatgaatat aattatactt ataacggagt
       61 gatttcgccc ttaagagaca gtatggaact gcttgttata aaaaaagctt ttgagctggg
      121 aaagcctatg cttggaatat gccggggaat acagataata aatgtagcaa tggggggtac
      181 tttatatcag gatatagcat ctcaggtgaa gaatagggaa gttataaagc actcgcagca
      241 agcaccggtt tggcacccta ctcatgatat ttatattgaa aaggatagtt ttgtatggaa
      301 gtctttcaat gaagatgcta ttgacggtga tgcggaagaa aagaatgcag cagaaaatga
      361 tgcgacaggc aacaaggctg caggtagcaa tgtcgcaggt aattttgttg taagggttaa
      421 ttccttccat caccaggcag ttaaagatgt agcacctggt tttagagtta catcaagagc
      481 agtagatggt ataattgagt ctatagaata ttcaggggaa gcttttattg tgggagtaca
      541 gtggcaccct gaactaatgt ggcagaaaaa ccgggtttat ttaaaattgt ttgataattt
      601 tataggagtg gcaaaataat agctaaagca ccagataatc cgaacaaaag gattgtaatt
      661 attttgtgca aacactgatt ttgctttatt attgtgtaag gagatgtggt attatgccag
      721 ttaagttaaa tattggagat atagtagaat taagaaagga acatccctgt ggaagcaaac
      781 agtgggaaat aactcgtaca ggtgctgata taaggataaa atgcatgggt tgtcagcatc
      841 aggttctaat tcccagacca aagttcgaaa aaagtttgaa gaagattatt gtatcggcag
      901 agtcatcaaa agaatcatca gagtcataga tatgtatgac cacgaatcag tcttatatgt
      961 gacaataaat cagtctggct ataaatctat cataacaaag agttgaaaat taataatagt
     1021 atgtaatagt acataatagt gcatctgaat aataaacatg gcaaaaagcc aatgttattc
     1081 gcatcaggtg cattttttta tgtttttcta gaaataatat taagaaacat aaaaaaggat
     1141 ggttttgcta catgtttgaa aacaataaca gatataatga ttacaataaa tacaatggcc
     1201 gtaatagaaa caacttttcc aggacatttc ttacttctct tataacctct ctgctagtag
     1261 ggacattact tattgtcctt atatggggca atgttatccc aattgtgcca aaaacgggga
     1321 tacccgggcc taccccggga cagagtccta atcttggcgg aggagcaggg gtgccgaatc
     1381 ctcagcaaat tggtgttaca ctagcagatg atgccccctc tgtagcgaat gtagtaaaga
     1441 gtgcatatgc cggagtagtg ggaatagcgg tattaagggc tgatgggaaa tcaatatttg
     1501 ataaaaatac tactgaaaaa tggggtattg gctccggggt tatagtaaat cctaatggat
     1561 atattttgac caaccatcat gtagcgggtg gaaaaagcaa aagactgatt gtatcctttg
     1621 ctgatgggag gaatctggat ggtgtaacct tatggtctga tcctgttctt gaccttgcag
     1681 tagttaaagt tagtgctgca ggattaacag ccataccacc aggtgatgca actacattac
     1741 agattggaga accggcaata gctataggca atcctttagg acttgaactc cagagaactg
     1801 ttacatcggg aattataagt gcccttaata ggacaatcag cattgagaca gagcagggag
     1861 taaactatat ggaagacctt atacagacag atgcaagtat aaatccgggc aacagcgggg
     1921 gccctctgct aaattccaga ggacaggtgg ttggaataaa tagtataaaa gtagcaaaag
     1981 ctgaagctat tggttttgct atacctatta atgttgccat acctattatt aataagttta
     2041 acgacacagg tacttttaat gaagcttata tgggcatttt tgcttatgat aaagaggcag
     2101 tgcctttttt ggatcaaaac attaagcttg ataatggtat atatgtagta gaagttgaca
     2161 agtccgggcc tgctgccgga gcaggtatta aagagggttg tattataaaa caggttgatg
     2221 gtgtaagtat cagtacaatg atggaactaa gggcgcttat atattctaaa aatccgggag
     2281 atacaatcaa tgtaactcat attgaaaggt ctacaggtga gactgttaca gttccaataa
     2341 agcttgcagc gaaagaaaaa gatggtctgt taacaaggta aatgttgtac taagtaatta
     2401 tttaacaagg caaatgttga taatttatat atttattcac agattgtgta tagtttgtgg
     2461 ataagttgga aaaatttata tataagcata tatataagta tgcataatta tatataagaa
     2521 ttagaaataa acaggtgggt aagcaaggaa gagctgtttc caattttgtc cctggttttt
     2581 gatgggtgga tgagcaagga agaactgtcc ccacttgcta aggaggatgt attatgaaga
     2641 atattgaaaa gcagaataaa gctgaaaagc agtgtaaagg aaatgatttt gaagtaacga
     2701 taaaagagta cttaaggcaa ggccgggaaa agttaattac tgacttaaca ggcactaggg
     2761 aggcaattaa gttaatagcg aaagacagaa caaaagaatt tatgctggcc accgacaagg
     2821 gattgaataa agaggaaaga gattatttaa ttgaagtgat tgtagccagc atgtaccaga
     2881 ctttttgcta tgggtatggt attggaaaaa ttgaggggaa atcaaataat agagtgtttt
     2941 tgtaagatcg caaatgtact ttgttgcaat gtgaaagagt attaggcgag cgtactttaa
     3001 ctgagattaa aactatctat gttataaatg attgattact ttttagtggg cattcctaat
     3061 ttttcactca caaaaaattc ttcttgacat atgaatcata acctgatact atattatgca
     3121 attatacaaa tccacggaag ccttaggcta atagctttaa tgttttcgac tggacatgtt
     3181 aaactaaata ttaataataa taggctaaat actatgatgg gaaaaagata tatccggata
     3241 attaagagaa ccggtggctg gtggaaaccg gtatgaagga tatatgtata gctcatcccg
     3301 gagttttatc attgaaggta gtaagattga aagcagcaag aacaagtaga tgataacgat
     3361 gactacgtta cagtcagggt gcctttaatg gctcccgctg aggatatacc aagagaataa
     3421 tgcatgacat gtataatgca tttcctttta actaacggta tgtcaaatca gggtggtacc
     3481 acgatgagat atatgcctct tcgtccctgc atattattgc agagacgaag aggctttaat
     3541 tttatagtgg ttaagaagat aagaaacagc taaaaataaa attgcaggat atttaatttt
     3601 gttgttttac tataaaaaga tagatatatc tggttagtag gtggttttat gaaaataaca
     3661 ggtgcacagg caatggttag aatattggaa atggaaaagg tcagccatat ttttggttat
     3721 ccgggagcgg ctatttgccc tttctataat gtattagttg attctacaat taaacatata
     3781 cttacaagac atgagcaggg tgctgcccac ggagcaagcg gctacgcccg tgcaaccgga
     3841 aagacaggcg tgtgtgttgc tacatcgggt ccgggagcga ccaaccttat taccggtatt
     3901 gctacagcct acatggattc aattcctatt gtagctataa ctggtcaggt gccaaccaac
     3961 cttatcggca gggatgtatt tcaggaagct gatattacag gcgcaacagc ccctttttgc
     4021 aagcataact acttggttaa aaatgcacgg gatttgcccg gaatcatgaa agaagctttt
     4081 catatagctt ctaccggaag gcccgggccg gtgcttattg atgtgcctat agacatacaa
     4141 aatgaatata ttgatttttc ttatcctgca agtgtagata taagaggata taagcctact
     4201 tataaagggc atccaataca gatcaagaga attgctgatg caatcagaaa atcgaagtca
     4261 cctgtaattt gtgcaggtgg tggtgtaata agcggagatg cctgcagcga gatgatgcag
     4321 ttggttgaga agtgcagtat acctgttgtt actacactta tgggcatagg agcaataccc
     4381 tcaaaccatc acatgtattt ggggatgctt gggtcccatg gcagttattc tgcaaattat
     4441 gccataaaca atgcggacct ggtatttatt atgggtgcca gggttgggga tagggctata
     4501 gtagctacaa acaaaatagc aaagcaggct gtaattattc atgttgatat cgacccggct
     4561 gaaataggga aaaatattga tgttgctata cctgcagtag gtgatgttaa atctatattg
     4621 gaggatttat tggatattgt tgataaaggc agcgtagaag catggaacat tacaatcagg
     4681 ggtgtcagag agaaacagga cttgaaagta agtaataaaa ataatgtaga tgaagaaggg
     4741 acagttaagc cttcatatct tttaagaatg ctctcagaca tggtggatgg cagcaatact
     4801 attgttacta cagaggttgg ccagaatcag atatgggcgg caaataacat gtccataacc
     4861 aaacccggca agtttataac ttccggcgga atgggtacga tggggtatgg attgcctgct
     4921 gcaataggag caaaggttgg atgtcctgat aatgtggtta tagctgtaag cggtgatggc
     4981 agttttcaga tgtctatgca ggaactgggt accataaaac aaaataagct gggggtaaaa
     5041 attataattt tcaacaattc gaggctgggt atggttcgtg agctgcagaa aatgcagtat
     5101 tgcgggcggt attctcaggt atttcttgac gaaaaccctg attttgttaa gttagcagaa
     5161 gcttatgata ttagcggaga aagaattgac aaaaattctg aaatcgggag tgctctggag
     5221 aggttgcttg cagatgacaa gccttatttg cttgaatgca ttgtagatcc tgaagagccg
     5281 actctttaaa atagctcaag taaatttaag caatataaac agtacaaaaa tagatcaata
     5341 attaagtgca gataaagggt aataggcagt aaggatgata agcagtacgg tgcgtaacgg
     5401 aacagtatgt atgttagtaa aatatcaagt aagagtgtga ggaggtataa aaatgggcaa
     5461 acatattctt tcagttttag ttgaaaatta cgccggtgta ctttccagag tatctggatt
     5521 gtttagcagg agaggtttca atatcgatag tctggcagtg ggtattactg aaaaccctga
     5581 agtatccagg atgactattg tagttgatgg tgatgattat atagttgagc aggttagcaa
     5641 gcaattaaac aagcttgtta atgttataag agtaaaaaaa cttgattctt caaattcggt
     5701 aagtagagag ctggctctaa taaaggtcaa tgccaacgct tccacaagat ccgagattgt
     5761 ccaaattgtt gaaatattca gggctaaaat tgtggatgta tcaaaatcca cgttaacaat
     5821 agaagcttcg ggagatgctg ataagattac tgcacttgag gatatgttaa aacaatttgg
     5881 cattagagag attgtcagaa ccggcctaat atctatcgaa agaggcaaca gacatattaa
     5941 agcaaataat aatgatgagg agtgattttt atggcaaaaa tgtattatga tagtgattgc
     6001 gatcttaatc ttttgaaagg taagaccata gcggttatag gttatggaag ccagggccat
     6061 gcccatgcgc aaaaccttaa tgaaagcgga gtaaatgtta ttgttggttt gacaccaaac
     6121 tcaaaaagga gacaacaggt tatttctgat ggactaaagg taatggatac tgcggaagca
     6181 gccaaagcgg ctgatataat tatgattctt acacctgatg aggttcaggc agcaatatat
     6241 aaagaaagca tagagcccaa tctcaaagat ggggatgtac ttatgttcgc ccatggcttc
     6301 aatattcact ttaatcaaat agtaccccct gatttcatcg atgtaataat ggttgcacca
     6361 aaggggcccg ggcatactgt aagaagccag tataaagaag gaaagggtgt tccttcactg
     6421 atagctgttt atcaggatgc atcaggaaaa gcaaaagaat atgcactggc atatgcagca
     6481 ggaattggag caggcagggc aggtatattg caaactacat tcagagaaga gactgaaact
     6541 gatttgttcg gtgaacaggc agtgctttgc ggaggcgtta cggaacttat gaaggcaggt
     6601 ttcgaaactc tcgtaaacgc cggttatcaa cctgaaagtg catattttga atgtgtacat
     6661 gaaatgaaac ttatagtaga ccttataaat caaggtggat ttggctatat gaggtactcc
     6721 ataagtgata ctgcagaata tggtgattac gtaacaggta aaagaattat tactgaagaa
     6781 accagaaaag aaatgaagaa aattttggaa gaaattcaaa acggctcatt cgcttccaga
     6841 tggatagctc agaataaggc aagtggaaga tctgaattcc ttgcaatgag aagaaaagaa
     6901 tcagagcatc aggtggaaaa ggtcgggact gaattaagaa aaatgatgag ttggcttaag
     6961 aaatagggaa ttacaataaa gcagtaatgc caggtgcata ttttctttac ttaaactgag
     7021 tgcacctaag aatgcttaac taataaccga tatccatcct gaaagcatgc taagggtggg
     7081 tatcggaata aaagagacaa acgataatta tcaaaatagc aaaacacatc gcgataatct
     7141 aaatatttaa ttaggttctg gaaacttatt ttgtacaaag gtaggaaata aatgtataag
     7201 attacatatc cagcggcaat gcttataaat tagatgacat aataagtaaa atttgtcata
     7261 aagatacaaa agctgctgca atgttggtga atgcaaatat gatagccaaa taacaaatat
     7321 gatagccaaa ttatagtaaa gatatatcaa aaagtagtaa gaagaggtgt atttatgtct
     7381 actcaaatta aaatatttga tactacatta agagatgggg aacaaacccc gggagtcaac
     7441 ttaaatgtgc atgagaaggt agaaattgca cgtcagctag aaaggcttgg agttgacgct
     7501 attgaagcag gttttgctat tgcttctcct ggtgattttg aagcgatttc cgaagttgca
     7561 aagaatataa aagatgcaac tgtagtaagt cttgccaggg ctcttgaaaa agatatagac
     7621 agggcatggg aggctgtgca gcatgcaaaa aggccatgta tccatacttt catagctaca
     7681 tcagacattc atcttaagta taagttgaaa atgacagaag aacaggttct ggaaaaggcc
     7741 gtggctatgg taaaatatgc aaagaaatat tgtgagcgta ttgaattctc tgctgaagat
     7801 gcaagcagga caagggttga atttttgtat cgtatttttg aagaagtaat caaagccggt
     7861 gcaacagtca ttaatattcc tgacaccgta ggatacgcaa ctcctgaaga atttggcaga
     7921 cttataagaa gtgtaagaga gaatgtttct aatattgaca aagttgacat aagtgtacat
     7981 tgccataatg atcttggaat ggctgtggca aattctcttg tggcaattca aaatggggca
     8041 acccagcttg aatgtacaat taacggtctt ggtgagagag caggtaatgc tgcaatggaa
     8101 gaactgatta tggcattgga tacaagaaaa gacttttatg gttataccca taaaattgat
     8161 acaacccaga tatatagaag cagcaagctc attaccagtt taaccggtat aatggtacaa
     8221 cctaataagg ccatagttgg agctaatgca tttgcccatg aatcaggaat acatcagcat
     8281 gggatattat cggagaaaag cacctatgag ataatgaatc ccgaatcaat aggccttaaa
     8341 caaaatagaa tggttcttgg caaacattcc gggcgtcacg cttttgaaga aaaactagaa
     8401 gaactggggt ataacctttc ggaagaacaa attaaaaacg cttttcaaaa atttaaagat
     8461 ttggcagaca gaaaaaagga tgtttcggac agtgatattg aagcactggt agaagaaaaa
     8521 gtggcaaaaa tacctgaaat atttataatg gaatcctacc agataagcag cggaaataga
     8581 cttgtatcaa catcaacagt tagtgtaagg agaaacggta atgtttctac ggaggctgct
     8641 acaggagaag gcccagtaga tgcagctttt aatgcaatgg aaagagcagt gggaatttca
     8701 ctaaagcttg aggactatag acttagagct gttacagggg gaaaggatgc ccttggtgaa
     8761 gttactgtaa gagtatccaa aaatgataaa ctatttacgg gcaggggtgt aagtactgat
     8821 attattgaag ccagtgtgaa agcgtatctt aatgcaataa acagaacatt aagcgaactg
     8881 ggcgaaaata taatcgaaaa taaaattctg aaataaaaag ctgaaaatgc atctttcaca
     8941 aatattagtc ttgacgaaag cagtatttta gtataactta ttttcaaagc attattaggc
     9001 aggcaggatc cacgcgagtt agtgcaggct ttgttataga ctttattata ggcttattat
     9061 aggtttgtta caggtttgtt ataggtttgt taattgaaaa tgattttgtg ataatgtagt
     9121 ttcttttatg ttttgtgcat tatatatagc aaggtgggtg agatgattga gacaggtgaa
     9181 aatatatgat acaactttaa gggatggggc gcaagctcag ggaatttcct ttactgtaga
     9241 agataaacta aaaatagctg aggcggttga cagactggga gcaagctata ttgaagcggg
     9301 taatccggga tcaaatccaa aggatctgga gtttttccag cgggttaaag gtataggcct
     9361 gaaaaacgca aaggttgttg cctttggaag tacacgccgt gtaaacatcg atgtgaatga
     9421 tgactcaaat gtaaaatctc ttctgatagc tgacacacct gcagtggcca tattcggtaa
     9481 aagctgggat tttcatgtgg atgaagttat taggacaact ttagatgaaa atttaagaat
     9541 gatatttgat actatagagt ttttcactaa aaaaggtaaa gaagttatat tcgatgctga
     9601 gcactttttt gatggatata aggcaaattc aaaatatgct atgcaaacta ttgaagctgc
     9661 ttgtaatgca ggggctgcat ctatttgtct ttgtgatact aatggtggct cctttcccaa
     9721 tgaaatattt aaaataactt ccagggtagt agaaagtcta aatatacctg taggaataca
     9781 ctgccataat gataacggaa tggcagttgc caattctatt attgccattc aggcgggagc
     9841 ttcacaagtt cagggtacca taaacggttt tggtgaaaga tgcggcaatg caaacttatg
     9901 tacaataatt ccaaatttgc agataaaggc agattatcag tgtatacctg cagagagcat
     9961 gaaatattta acatcagtgg ccaggtatgt aagtgaaata gccaatgtga ttcacgatga
    10021 aagagaaccc tatgttggcg gcagtgcttt tgcgcataaa ggcgggatgc atatagatgc
    10081 cgtatttaaa aatcctgttt ctttcgagca tataaatccg gaactggttg ggaacaaacg
    10141 tgtaatattg atgtcggaag ttgcgggcag aagcactatt atgaaaagaa ttaacgaagt
    10201 ggattcatcc atatgcaaag actcacctga agctaaggca ataataaata aaatgaaaga
    10261 actcgaacat gatggttacc agtatgaagg agccgaaagc tcttttgaac ttgtcataag
    10321 gaaaatattg ggtaaataca caccattttt taatttaaat gaatttaagg ttatagtagg
    10381 cgagccttcc ataaatagcg taaactcttc agctatgata aaaataggtg tgggagatca
    10441 ggctgaaatc actgcagcag agggatgcgg tcctgttaat gctctggata atgcccttag
    10501 aaaagcactg gaaagatttt accctgaaat aagacagatg aaattgacag attataaggt
    10561 tagggtttta gattctacgt cggctactgc agcaaaagtc agggtgctta tagagtctgc
    10621 agatggaaag gatgtatgga ctacaatagg agtttctacg gatataattg atgcaagctg
    10681 gaaggcgctg gtagattcta ttgaatacaa gttaattaaa gatagaattt aaaggacgca
    10741 ctttaattta tgaagaaaaa tactccaaaa gctattgtta cggtgcttag agacttcaca
    10801 attgcttcct cctgtaattt cttcataaat atagtatgtg cctgaattta tagagagtgt
    10861 agagaaaatt aatataagat taaatataat atgaaaatta aatatgaaat ttggataata
    10921 gtctgagaaa gaaggtctgt gttttgaaac ttgccggaaa cctgattaag ggcacaaaaa
    10981 ttattaggag agcatccttt gagaaagaag accaaagtat atcattccat gatatgctgg
    11041 aagagagcct tttagcatta tgtaaagaac tggatatacc ggttcccata tggatgaaaa
    11101 agaatacagg tgaatttgcc atgtttcgca ggacattttt taccagcgag caatttacag
    11161 aaaaagttgg gtttgaccga tttgaaataa ggatggagta gatggttaag actaatactt
    11221 aaacgaatat gaccgatttt aataagtatc gtccaaataa tcatagattg cagaactaaa
    11281 attctaagtg cagattccgt aattagtgct gcaagaaaac cggagtatgg aatacatggc
    11341 acaaccaagc ctgaatcaat aggatctgcc accagtcagg gttgtaccag aatattcagt
    11401 gatggagttt gaatgatgcc aatatttccg tcactcatag tgcagccaca tgcaaatgat
    11461 ttttacgatg tgttcttttt gaagaacttc atatataaca cgatgttgat ggtttatacg
    11521 cctggaataa gcacctgcta aatcgcctac aagtttttca tacggtgggt aacctgcaaa
    11581 agggtcattt tttaatgttt ctaaaatgct gattattttt tttgaaaaac cagcttcaca
    11641 agcaatcctt ttatctttta gtgcttgctt tgtataaaga attttgtatg attcactcat
    11701 catcatcctc cactagatcc tctaatggag tatttaaccc acgtaagatt ttttcacgca
    11761 tgcctggaat ggatattaaa tatagtgttt cctgaataga acgatagtcc tcttccgaaa
    11821 taactacaat gttccccgct ttacctgtta catagacagg ctcattagta acaattacat
    11881 cttccatgat tttaaaaaaa ttgtttctgg cagttgttgc attgataata gacattgtta
    11941 cacctccaac gtacgtgata gcgtcattta tcctgtacat attatatgac atattgtact
    12001 taattgtcaa tgattagttc ctgcgggtga gagtatgttt tagataaaaa caggatgaaa
    12061 atggagcatt ccgaataaag cttacctttt attggtggca gttattaaat gtcataattt
    12121 tgataaattc ttttgctgct gttgaaagag gtatttgttt catatttgct atgcccagtt
    12181 tcctgagagg aagtttttca gttgttttta tctggaaaag ttcacctgat ttaatggctt
    12241 cagcagcact ctctttaagt acatgtgcta ttcccaatcc tatctttgca aattcaacaa
    12301 gaagctctat actttccagc tcaatttccg gaaccatttt gactccattg cttgctagaa
    12361 attcatcaag caaccttctt gttgagcttt cctgctccaa ggtaagaatc gggtaatttg
    12421 acagttcaca caacttcaca attttgtcgt taagcccaga aaacctttcg gatgcaacaa
    12481 atatatcttc tactgtaaga aaatctataa cactaatgtt cttttcgttt actggaaggg
    12541 taactattcc aaaatcaata attccatttt taagcacatg tattatctga tttgaagtcc
    12601 tgttgaccac acgaattttt atatttggat accttcgatt aaattcctgc agataaggta
    12661 tcagataata cctgcatata gtatctccta tacctatacg tatttcacca agctcgaggt
    12721 ttttaagctg tattattttg tcttctcctg ttttgatgaa attataggct tgttcaatat
    12781 ggctataaag cagttgacct tcaggcgtaa gtttaattga gcgtgacttc cggtaaaaaa
    12841 gtctgctacc tattttggat tcaagattct ttatagcctg acttactgcc gattgtgaaa
    12901 taaaaagcct tgcagcagct tcagaaaaac ttcctgaggc tgccgcctga tagaaaatct
    12961 tatataactc aaaattaata tccatctccg tacaccacct gtatatataa gtaaaactta
    13021 agtatactat tattattatt aattttacta atcaattgta ccatactata ataaaaagta
    13081 aaggagatac tgaattaaat tgctttatta gctttggaag atataaaaac agggaattgc
    13141 gaggtaattg ctatgaatat gaatgttgtg aataataata aaaataatgt taataacgtc
    13201 aagcgtattt ttgttgaaaa gaaaaaagag tttaatatag aggggcaggc cctttacagg
    13261 gacttcaaat ataatctggg aattaaaggt cttacgaatg taagaatagt aaacagatat
    13321 gatgttgagg gtatttcaga tgaagactat agagcttcaa gaaacactat attttctgaa
    13381 ccgcctgtag atatagttta tgatgaagtt ttttctacag gagagaatga gaaggttttt
    13441 gctgttgagt atctgccggg tcagtatgat cagagagccg attctgcatc acagtgcata
    13501 cagatactga caaggggtga gagaccggtg tgcagaacag caaagctttt tattattgaa
    13561 ggtgatgtat ctgatattga atatgaaaaa ataagaaatt attgcataaa ccctgttgaa
    13621 tgccgggaag cctctttagg aaaaccgtca acacttgaaa tggttgttga tgctccagaa
    13681 gatgtgagca ttctccaggg ttttatcaat atgacagatg aagaactgga atcacttata
    13741 gataatatgg gactggcaat gtcatttgaa gatctggaat tttgccgcaa ttatttcaga
    13801 gacattgaga aaagggatcc tacagttact gaggtaagga ttatagacac atactggtct
    13861 gatcattgcc gtcatactac attcctgaaa gaaattaaag atattgaaat taaagatggc
    13921 aggtacactg taccgataaa atcagctttt gatagctata tcagctcaag aaaattcgta
    13981 tatgaaaata gaaaaaagga catatgtctt atggacatag ctataatagc tatgaaggaa
    14041 ctgaaaaaaa gaaatctgct gaatgatctg gatgaatcgg atgaaattaa tgcctgcagt
    14101 attgaagtta aggttgatgt aaatggaaga aatgaagatt ggctggtaat gtttaaaaat
    14161 gaaacccata atcatcctac cgagattgag ccttttggag gagcggctac atgtcttggg
    14221 ggagctatta gagatcctct atccggcaga tcatatgtat accaggcaat gagagttaca
    14281 ggcagcggag atccaaggac acctgttgaa gatacactgc ccggaaaact cccgcagcgc
    14341 aagataacaa ccggtgctgc agcaggttac agttcttatg gcaatcaaat aggaattgct
    14401 acagggcagg tagtggagat ttatgatgaa gattatgttg ccaagagaat ggaaataggg
    14461 gctgtaattg gggctgcccc aaaaaacaat gtgttaagag aaaaacctgt acctgatgat
    14521 attgttatac ttcttggagg aaggaccgga agggatggct gcggaggagc aacaggctct
    14581 tcaaaggagc acagtaaaga atctttagtg aattgcggag cagaagtcca gaaaggaaat
    14641 cctccaacgg aacgaaaaat ccagaggcta ttcagaaacc ctgaagtgag cgccttaatt
    14701 aaaaaatgca atgattttgg cgcagggggt gtaatggttg ccattggtga attagctgat
    14761 ggaattgaga taaacctgga tgcagtaccc aggaagtatg agggccttaa tggtacggag
    14821 cttgccatat cagaatcaca agaaagaatg gcagtggtag tttctcctca aaatgtaaat
    14881 aaattcattg ctgctgcagc tgaggaaaac ctggaagcta caccagttgc agttgtaact
    14941 gaagttccgg aactaaaaat gagatggcgg ggtaagctaa ttgtagatat cgaccgtgag
    15001 tttttaaata gcaatggtgc aaaaagggaa actacagcag ttatagcttc accggttgag
    15061 gataaaaatt ttttcagaat ccaggcgggt agtcaggaaa gaatggaaga cgaaccactg
    15121 caggccaagt catctaaatc ccgagatgat atccaggata acataaagag tacatggctt
    15181 aataatcttg gaagacttaa tgtttgcagt cagaaaggac tggttgaaag atttgatagt
    15241 accataggtg ctggaacggt gctaatgcct ttcggcggaa aatatcaatt aagcccttcc
    15301 gaaggtatgg ttgctaaaat acctgtactg gatggggata catgcacagg tactgttatg
    15361 acctttggat acaaccctca actggcaaaa tggagtcctt tccatggagc ggtttttgca
    15421 gttcttgagg cgatttctaa aattgctgca attggaggaa attataaaaa tgcaagactc
    15481 agtctgcagg agtattttga aaaattaggt aaaaatcctg agaagtgggg acaaccttta
    15541 tcggctctct tgggagcata ttatgctcaa ataaagctgg ggattcccgc aataggtggt
    15601 aaagacagca tgtccggcac atttaggagt gtattaagtg atatatccgg ggacatatcc
    15661 gaaattactg aagaaatgaa tgtaccacct acattagttg cgtttgcagt gagtgttatt
    15721 gatgtaaatc atgtaatatc ttcagagttc aaaaaggcag gaagtaaggt tgtaatgctt
    15781 catatagatc gtgatattaa tgaaatgcct gattttgagc agcttgataa aaactatacc
    15841 agaatacatg aacttattgc agatgataaa atacttgcct cagccgcagt aaaaaccgga
    15901 ggtattgcag agaccataag caagatgtgc tttggtaata tgataggatt taaatttgaa
    15961 ggacatgtag aaaaagaatt acttttcaat ccagaatatg gttctattat tcttgagata
    16021 ccagagagag aggatttgga tatccttttc caaggactga aataccatgt gctgggacat
    16081 actgcagaaa agcctgagat aaacgtaaat gtaaatgtaa gcggaaacgg aagcgaaatc
    16141 ggaagcgaaa tcggaagcga aatcggaagc gaaatcggaa acggaagtaa taatgtaagc
    16201 gtaagcaaag tagcaataag tcttgaggag gccttgcaga gatggcagga gccccttgag
    16261 gggatattcc caactaaagt tgaggcgaaa tttccaacca aaggttccgg aacacttcca
    16321 acaaaagctg ccgggacatt tcctgagaaa ctggattgga actacaatct aaaagataac
    16381 ttaaattaca acaattgcaa caaaataaaa tcaactagaa ctcttaagat tgtaaggcca
    16441 agaatattta tacctgtatt tccgggtacc aactgtgaat atgatgctaa aaaagctttt
    16501 gaaaaagcag gaggatttgc tgatgtaatg gtaatacgca atcttacctc aaaggatatc
    16561 gaggaatcta tagaaatgat ggcaaagaaa atagataatt cacaggtaat aatgattccc
    16621 ggaggtttca gtggaggaga tgaaccggac gggtccggca aatttatagc tacagctttc
    16681 agaaatccca gggttaagga tgcggtaatg cgtttgctta aagaaagaga tggtcttgta
    16741 ctgggaatat gcaatggatt ccaggctctt ataaaacttg gattagtacc ttatggagaa
    16801 atcagggata tgactgatga aagccctaca ttgaccttta atactatagg cagacatgct
    16861 tcgtgcatgg tgtacacaaa gatagtatca aatctttcac catggctgtc taatgttaag
    16921 cagggtgaaa tgcatactat agcaatatct catggagaag gaagattcgt tgcttcacag
    16981 tcagatctag ctcttatgga aagaaatggc caaatagcaa cttgttatgt agaccctgca
    17041 ggcaatccca cttatgatat aagatttaac cctaatggat ctgtaatggc tatagagggt
    17101 attaccagcc cggatggaag ggtgtttggc aagatgggac attctgaaag aatcgggaaa
    17161 aatgttgtga aaaatgtacc gggagaaaag gaccaaaaaa tatttgatgc agggatagaa
    17221 tattttagat gaatatggta tattacggtt aatgtaagtt gaaagacgtc aaaaatattc
    17281 caaaggatgt gatgagtata tgtgtatata tgagcgatta aaggaacttg gtattgaact
    17341 cccaaaaacg cctgcacctc tgggagtata ttttcctgct aaggaatttg gaaataaatt
    17401 tatctacatt tcaggtcagg gaccaagtgt aggagggaaa cccctttgtg taggtaaagt
    17461 agggaaggat ttatctcttg aagaaggtca gaaagctgca tatgcttgta tgctgaatgt
    17521 gttgggcgta cttaatgata aaactggaga ccttaacaga gtaaaaagtg cagtgaaaat
    17581 attgggtttt gttgcatgtg cgaacgactt ttatgatcag ccaaaggtaa ttaatgcagc
    17641 atcaaaattg cttgttgatg tctttggaga agaaatcggc aaagcttcca ggtcggcagt
    17701 tgggactaat gcgcttccag gcaatattcc tgttgaaatt gaggctattt ttgaaataga
    17761 gtaaaattaa gcaatgtata gcaagttgta gataagtaaa ataacactac ttgcttatgc
    17821 aaaaactgta tatttattgt gtctatatta atacacttcc tcatatattt aaaatgtaat
    17881 atgtggaggt gcttttttgt gatgagtagg aagataatag gggtttcttt gtgtgtactt
    17941 attttattaa gtattattca aataataata gattgcagat ccgaaactga aaattcaaat
    18001 gctgtaatta gtgctgcaag aaggccggaa tatgttatct ttattgaagt agaagataaa
    18061 acactttatc tcctggaaga tggagcgtgt ataaaaaagt atcccatagc cagcggcaag
    18121 tccggttatc cttcgcctat aggttactgg aaaattacga ccaaggaaaa atggggagaa
    18181 ggttttggag gaaggtggat ggggcttaac gtaccttggg gagagtatgg aatacatggt
    18241 accaacaagc ctggctcaat aggttctgct gccagtcagg gttgtatcag aatgttcagt
    18301 gatgatgtaa aagagttata tgatatagtc cctcttggta ctagggttgt aatagttaac
    18361 ggactttttg ggccttttgg aagaggtttt gctaatatcg agcctgggga tagaggtgct
    18421 gatgtaatgg aaatacagcg aagacttaag gatcttggat atttcaaagg ttggttatcg
    18481 ggtgtttatg aagatgatct taaaaatgca gttcatcggt tccagaaaga taagggcctg
    18541 cctgtaataa ataccataac aagggaatgc tgggaggcta tggggttcag agagtttgag
    18601 taataggcaa atggggacgg ttcttgcctg cttgcctcag agaggtttct tgcttcctta
    18661 catttcctta tattcctttt aaatcaaaga caggagtgta tatggtgtca gctgatgata
    18721 attcctgtac atatccattt tccaggccaa tatcaaagaa ataatcaata atggtttcat
    18781 attcactttt gcggatatga cgattcagtt ctttataatt ttccgcctta tatacaggag
    18841 tatattgtga catgaggctt atatacacag ttttagggag attttcactt atccacttga
    18901 gaatattctt cgtctca
//
LOCUS       DAOSDD010000063        18837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1211_length_18837_cov_5.043020, whole genome shotgun sequence.
ACCESSION   DAOSDD010000063 DAOSDD010000000
VERSION     DAOSDD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18837
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_18837_cov_5.043020"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PK733_07535"
     CDS             <1..377
                     /locus_tag="PK733_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HOJ10428.1"
                     /translation="LGLAQWNDRGKVDISAKVWRYTGEKWSRQSEELPLHRVLDLAIL
                     VCRAKLHFTEAYRYEKLYDTEKPIIDRVGLQGDAMTVAVCTDNEKIDEDIKLFNQALS
                     NDDELIGERLRTLSRILKEMGY"
     gene            620..979
                     /locus_tag="PK733_07540"
     CDS             620..979
                     /locus_tag="PK733_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOJ10429.1"
                     /translation="MNKKSRKELLEEYKRLKTYMGVIQITNNATGKIYIAPYPNLKNK
                     WLTIQGQLNMGMHVNSQLQKDWNALGANAFTYEVLEQKEADEVADIKWELKQMEKPWL
                     EKLQPYGDKGYNKPPSE"
     gene            1283..2467
                     /locus_tag="PK733_07545"
     CDS             1283..2467
                     /locus_tag="PK733_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ10430.1"
                     /translation="MINYCNVKFKNPMVIASSPLTSNIDRLIEAEKAGAAAVSTKLTF
                     IKQPFYGKLRMHTYLNNGSIICYDRRNDIDESLKLIDEAKKRTSLKLFANITHDGMDM
                     DGWTLLAKKHEEAGADIIEANMVCPNVGLSTKNIRGDSAISDHEHGGAVTGQNPERIT
                     EIVKVLKNAVKIPVIVKLTPNVSDIGVTAKAAEAAGADGVCVAGAQSSLPLVDIYNEG
                     KPQYHLLNGVSHGSLGGPATKLMGFSLVANIAKKTNLPIVGGGGLETWQDSIMMMMWG
                     ADLVTVCTAAMWYGWENITKQVKGMEKYLEEMGYSDYTQITGKSLQHLRSSSDLDALK
                     GWAAVDKEKCIGCGKCEKPAHCTAVKIENKKSVIDPEKCLGCGICEALCPTKAISMHI
                     AE"
     gene            2915..3493
                     /locus_tag="PK733_07550"
                     /pseudo
     CDS             2915..3493
                     /locus_tag="PK733_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            3508..4179
                     /locus_tag="PK733_07555"
     CDS             3508..4179
                     /locus_tag="PK733_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10431.1"
                     /translation="MHKHNKANERDQHQKDILESNNILTETNPEKIMWETLPDGDMGL
                     RTPPGGGPISEEQRFSALSDGSFYCVYRSVDGHPVYTYSRDGGHTWDVPQYKRYYDGR
                     LMKHPRAANFAWKCSNGKYLYWYHNHGGTGYEDRNPAWICGGIEQDSPNGRVIIWTQP
                     EILLYEDDTFVRMSYPDIIEEGDRLFITETNKNAARIHEIPAKFLEKLWNQFEISYVE
                     EDGLL"
     gene            4343..4537
                     /locus_tag="PK733_07560"
     CDS             4343..4537
                     /locus_tag="PK733_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10432.1"
                     /translation="MWDYGGKNPMPIDLMQYQLDKYYEWLKSGEIDGIIFCSNCIADL
                     GLEAVDITREWIKKVGGERL"
     gene            4714..6648
                     /locus_tag="PK733_07565"
     CDS             4714..6648
                     /locus_tag="PK733_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ10433.1"
                     /translation="MRKRKLIIILIWLPFVLIVLLLGGYSSEVYSNRSKVVEGIIDLN
                     QRELDEEVIKLDGQWEFYWNQLLEPGELKEIGIQRSGYIDIPSSWNRYIINDKELSGD
                     GYATYRLLFKTDKNGRLGIKIPRILTSYNLWVNEELIAAAGMVGKSREAMTPQYLPQV
                     AFFEAKQGKNEIVIQVSNFYHRSGGILESIFIGNEKKIIGLRYKGIACDLLLFGSLTI
                     IGFYHIALFLFRKKDTTPLYFGLFCLFIGIRTLLVGERFLIYLFPDFNWEIAHKIQTL
                     TFYLGVPSILIFFKMVFLNDFNTKFVKIIYMVGITFGCLVLLTPAKFFTLLNPVYQIF
                     SFIVIVYIISTFIKILLRKEKGITLIIAGALALILTSLNDIVFLSIWMNDNRSPLLRT
                     LFRTGNLSSVGQLIFVFTNSLVLAKKFSNALEQQEVMTAQLKEVNLNLDELVIKRTKA
                     LEESKVKIECQKLELENANRKLQMLSLKDPLTGLGNRRYYDDTMLMEWDQGLRCKRPV
                     SLMMIDIDFFKSYNDCYGHSAGDDCLIQVAQAIKDFIKSTGDLAVRYGGEEFVVIMPE
                     TGKPEAIKKALLLRKTIEDLNIPHKCSPVSPWVTASIGVTSMIPDIECSPNELFLAVD
                     KALYQAKAAGRNQVKFSAET"
     gene            6918..7151
                     /locus_tag="PK733_07570"
     CDS             6918..7151
                     /locus_tag="PK733_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOJ10434.1"
                     /translation="MQALNYSLEEKVNVSLAISLFAGRQVSLAKAAKLANKSLTSFMD
                     ILKKVNIPWCEYTEDFKNADDFAVNLLLQESES"
     gene            7473..8552
                     /locus_tag="PK733_07575"
     CDS             7473..8552
                     /locus_tag="PK733_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10435.1"
                     /translation="MNENNAGKDIIKNNNYSTYTNTESYDSYINSNNKSHSDNNNSNN
                     KNNNNNNNNNNRIMISLTRELKQNYQLYILVLPAVLYFLIFCYIPMYGAQIAFKEFVP
                     HQGILASKWVGFSQFERFFNSYQFKTLFSNTLLLSLYNLVAGFPVPIILALMLNQLSS
                     SKLKKVVQTATYAPHFISVAALCGMMVVMLSPTGGIFYKILEAIFGENLPIPLGNPRW
                     FRTIYVVSGIWQHAGWEAIIYIAALTSVNPDLYEAADIDGAGKLKKIIYIDFPSIIPT
                     AITLLILNAGKIMSIGFEKVFLMQNSMNLATSEIISTYVYKIGIIGSEFSYSSAIGLF
                     NSIINLILVITINYISKKYKETSLW"
     gene            8564..9442
                     /locus_tag="PK733_07580"
     CDS             8564..9442
                     /locus_tag="PK733_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10436.1"
                     /translation="MKRQATQKDKIFDFVLNIIMVVILIITLYPLYFVVIASISNPDF
                     TYAGKVILLPKGITFAGFEIILADGRIWERYANTIIYTIVGTAVNLIITLPASYALSR
                     KSFKAKKYINLFILFTMFFSGGLIPTYLLVKGLKLIDSMWAIILVSAVSVWNLIIART
                     YFSGNIPDELEESALLDGCSHIKFFFLVALPLSKVIVIVLALFYGINHWNGYFNALIY
                     LNTPQKFPLQLFLREILIQQQSMAMDPELARDMRYTANLIKYTMIIVATGPVLIIYPF
                     LQKFFVKGVMIGAVKG"
     gene            9532..11208
                     /locus_tag="PK733_07585"
     CDS             9532..11208
                     /locus_tag="PK733_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10437.1"
                     /translation="MYIVKKRFYKKAALMALVLCILLTATACTVNNKASTNSSNESND
                     ATTTKQQQSNFNATGYPVVNEPVTIKIVAKHSIGKDQYKYGEMQLFKEISEKTNVLVE
                     WDEIAGSAFAEKKSLIFASNDLPDAFWGEGMSDSDILTNKDFFIPLNDLIDKYGVNIK
                     KVFAKRPETKRSIIASDGNMYSLFRVRELYFPAGRTTWGINKKWLDKLGLEVPKTTDE
                     FYDVLKAFKTQDPNGNKKQDEIPFIFHHAMGSGSVRSAADFYPAFGVYENTSNSDLTY
                     HLMLRNGKVVFTPMDPGYKEALKYLNKLYAEGLLDREVFTTDSSTYYAKTRNEEDLVG
                     VIIDWTIDSAVGVDRAKNDFVQLGALIGPNGDQGWGIVSGSQIGRHMFEITSKNQYPE
                     ATMRWVDEFYDPINSVQAFFGPIGEMIKKEGEKLIALEPEEGVSYGSWKWGRTCADSG
                     AYAALKEYSSFFIPPEQQVARAKAEEYVAEHLQKEPFPNIFFSTEDVAALKKILVDIN
                     AFVETSRAQFVTEGNIDKKWDEYVQQLEKMKINDALTILQRAYEQYIKNN"
     gene            11789..14032
                     /locus_tag="PK733_07590"
     CDS             11789..14032
                     /locus_tag="PK733_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10438.1"
                     /translation="MKAIQKKAFLYRYIISFLLILIVPMVCLLFYIANLIKSNMQKDL
                     MSADYNNTVYIHNSVDNSIRKFENLALLTMSNSNLKTYFLDQSYFNYYQAVSTLKYIK
                     GTNELLNVLVLYNKSNDLVISNFGTIPKQYFGNKVLSATLDNKIALKDFIETVIQPVY
                     TYGYVNISGIGERRCLLYFYPTTSDFEQSKSIIMMIVDVQNIIDLFDEQYNSVSGSVF
                     ILDESNNTVVQYGNANEKILNNYLQYNNRLLNGSEVFLYQNNKYCVSGITSLKTGWKY
                     ISIVSFSIANREFTYNMYIFYFVFIILFIISGLGIYISIRLNYNPLLKLKKQIEPIVE
                     TKSLNEVETIRTAVNMLINNVDRMSSWMKDIQPAVHNYLIQQMLFGHKTDIDDFKQLS
                     KEICFSFYKSRFMVGIIKLKRADYEVVISDLMKISNDNITVYYLNFPVDDMLLAVFNL
                     EEHDYEKVIAFLNDFVEHINMKNKYDIHISLGRLNELQDVSKSYFEACIGELFKKFGS
                     QNQRVYCFNDIMKSTESPSTGFCPGHIRDFELNILRANIEYIAKFPSIIMQEISTNYL
                     PEYYIDYVIYELIRVVISSLAGDASQEVLLDRIWDYIFARSPISSTNVLEDVISFICN
                     TIIQHIRKNPITKDSTLNEILKYIDENYYKPDFSIQLTASAFNMSPSWLSHYFKQNMY
                     ITIAEFVQNKRIKYSQQLILENECSTNEISEQLGYCNVSSFIRAFKKTVGMTPKQYKE
                     TFGNSKP"
     gene            14087..15919
                     /locus_tag="PK733_07595"
     CDS             14087..15919
                     /locus_tag="PK733_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ10439.1"
                     /translation="MNRKILPIEVNVCDKFWSTIIERNSEVTVPVCLKRCEETGRISN
                     FANAALDKKGSFQGRFYNDACLYKVIEGVANTLKLNPNPVLEETADNIINKIAAAQMN
                     DGYIMTYFQLQEPENRWTDMGMHEMYCAGHLLEAAIAYYEATRKVKLLCVAIQFIEHI
                     MCLFGEGKRHWVPGHEEIELALVKLYDKTGEKKYLEFSKWLIDQRGKGLSRGRDYSQE
                     FYNTKYNQDDIPFVSQRIAKGHAVRAMYLYSAAADIAYETGDNSYISALLGIWDNVVQ
                     KNMYITGGIGSTKFNEGFSTDYNLPNKTAYCETCASVGMVLWNYRMWRLTEEAKYFDI
                     AERAMYNGVLSGVSITGDLFFYDNVLESDGDKKRSPWFTTSCCPTQIARFIPTVGSYF
                     YMNNNNCLWINMYGSSQAKIEIDKQDIIVIQKTDYPYDGKVEIIFHTKNKFSAKVKLR
                     IPGWCDNWRLHVNSAIKDAAMDKGYLTLDREWSDGDVITLALDMPVKLNYSHECVEEN
                     KGKAAISRGPLVYCIEEIDNMQYDDVKIDPHGEFSDISATINTADNINTEDPANDVNT
                     VNSAETRFIRWDVQNIDAVAVPYHLWNNRGQGKMKVWIPLIPSC"
     gene            15974..17905
                     /locus_tag="PK733_07600"
     CDS             15974..17905
                     /locus_tag="PK733_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ10440.1"
                     /translation="MYEKIYSKLPLGQVLADGWLKVQLNMQAKGLTGNIESIWSDLGP
                     DSGWLGGNGESWERGPYYCDGLVPLAFLLKDNVLLEKVEKWIGWTLQSKKADGFFGPG
                     SNDDWWPRMVMLKALMQYYEAKPTPDILSLMSDYFQYQLNKIDDKPLTDWGKFRASEN
                     IDAILWLYEKTNKSNLLNLADKIFKQAFNWSDFFKDFPYEKPIGNYMNWQEVHEAHLP
                     VNERTMRYPDYMKSHIVNVAMAVKYPALAMRLYNDQEQKEILKKGLEDLYKYHGVANG
                     MFIGDEHLNGNNPSNGSELCAVVELMYSLEKVLEVIGDTFYADLLEKVTFNALPATID
                     KEFCSHQYLQQANQVLCTIAKRDWYNNLDDSNIFGLEPNFGCCTANMHQGWPKFVQNM
                     WLKTNRGFAAMLYGPCTLITELNDRNKIKIHEKTNYPFEGNIDFNICMEAPETFELQL
                     RIPGWCKTFSLFVNSSKVSYTIRDGFIHIERLWKDGDCVKLILDMPIIKSFWANNSVS
                     IERGPLLFALDLEERKKLINRKPPFADWELYPHKKWNYRLLLNNMNVKENFNNLNIHD
                     NKDEQSESSNPFKEKNKFVEIEVYAAPCEEWKIVRNSADLPPESPAAVDSDNCIKLRL
                     VPYGSTMLRISQFPWGDIQ"
     gene            18017..18811
                     /locus_tag="PK733_07605"
     CDS             18017..18811
                     /locus_tag="PK733_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ10441.1"
                     /translation="MERKMKIYLIDLDGTLYRGNEPIEYAAEFIDYLNRNNRKYLLTT
                     NCPLNGPEEIVMKLKTMGIAATTDKVLTSGMACRDYLLEHYFGKNVFVIGSPSFRKLL
                     CNSGIQLTDEYSDVVVIGYDQQTTYSQIKQACINILNGAEFISTNMDNVIPFGKTFIP
                     HTGAITSAVGYAVNKEPVVIGKPNQHMFNSAVKILGCNREDCIVIGDRLDIDIQFSYN
                     NGIPGYLVFTGVAKCTDVENSIIKPDKCFNNLRDIIEMERRNFDVL"
BASE COUNT         6547 a         2843 c         3835 g         5612 t
ORIGIN      
        1 cactaggatt agcacaatgg aatgaccgcg gtaaagtaga tatttcagct aaagtgtgga
       61 gatacactgg agaaaaatgg tcaaggcaat cagaagagct accgcttcac cgtgtgcttg
      121 acctggcaat tttggtttgc agagcaaaac ttcatttcac agaagcttac cgttatgaga
      181 aattgtatga taccgaaaag cctattattg atagagtagg tttacagggt gatgctatga
      241 cagtggcagt atgtacagac aatgaaaaaa ttgatgaaga tattaagttg ttcaatcaag
      301 ctctcagtaa tgatgatgag ctcattggtg aacgattgcg taccttatca agaattttaa
      361 aagagatggg atattaatct ggaaagagat gggacattaa cctgaaaaga gatgggatat
      421 taacctcaaa agaaatgtga tatttatcta tacacagggg aaaaaatgcc tttataataa
      481 ttgcctgtaa ttgcctgtaa agctaaatca gctagggtag ataagacaac tgcagataga
      541 caatttcaga tagacaattg cagataagat aattatttat aaattcaatt attatgtaaa
      601 cctgaaagga tataaaataa tgaacaagaa aagccgtaag gaactgctgg aggaatacaa
      661 gcggttaaag acctacatgg gtgttattca aataaccaac aatgcaaccg gtaaaattta
      721 tatagcccct tatcccaatt taaaaaacaa atggttgacc atacagggcc agcttaacat
      781 gggcatgcac gtgaattctc agctgcaaaa ggattggaat gcgttggggg ccaatgcctt
      841 tacctacgag gtgctggagc aaaaggaagc agacgaggtt gccgatatta agtgggaact
      901 taagcaaatg gaaaaaccat ggctggagaa attgcagccc tacggagaca aaggctataa
      961 caagccgcca agtgaataag taccattcaa tatccataaa tgaatgaaac ttggtgtgct
     1021 aaagggcata tgtggttctg ctgaaaagcg gcaaaaatta attttaaatt tcacttgcca
     1081 taatatgata ttatgttgta taatgtaacc aaacggttac taatgaaata aaccttgcac
     1141 aaaaacgtta agaaaacatt aaagcatgtt taaatgtgtt aaaatgtgtt gaagtatgtt
     1201 gaaaagtgcg aggatttttt taggagacag gtaactaacc agttacttgc aaaatttaaa
     1261 atgtaaacga ggagagtacg acatgataaa ttattgtaat gtaaaattca agaatcctat
     1321 ggttatagct tcaagccccc taaccagtaa cattgatcgt cttattgaag ctgaaaaggc
     1381 aggtgcagct gcagttagta caaaactgac ttttataaag cagccattct acggaaaact
     1441 cagaatgcat acttatttaa ataacggaag tattatttgc tatgatagaa gaaatgatat
     1501 tgacgaaagt ctaaaactta tagatgaggc taagaaaaga acttctctta aactttttgc
     1561 aaatataact cacgatggaa tggatatgga tgggtggact ttacttgcca aaaagcatga
     1621 ggaggccgga gccgacatta tagaagccaa catggtttgt cccaatgtag gtctttcaac
     1681 aaaaaatatt cggggtgaca gtgctatttc cgaccatgag catggagggg cagttacagg
     1741 tcaaaacccg gagagaataa ctgaaattgt taaagtacta aaaaatgcag ttaaaatacc
     1801 ggttattgtg aagctaacac cgaatgtgtc cgatatagga gtaacagcca aagctgcaga
     1861 agctgcaggt gcggatggcg tctgtgttgc aggagcgcaa tcatcgcttc ctcttgttga
     1921 catatataat gaagggaagc cccaatacca ccttttgaat ggtgtttccc atggaagtct
     1981 tggaggacct gcaacaaagc ttatgggttt ctcacttgta gctaatatag caaagaaaac
     2041 caaccttcct attgttggag gaggcggtct tgaaacctgg caggacagca taatgatgat
     2101 gatgtgggga gcagatcttg ttactgtatg tactgcagct atgtggtacg gatgggagaa
     2161 catcacaaaa caagttaaag gtatggagaa gtatctcgaa gaaatgggat atagtgatta
     2221 cactcaaata accggtaagt ctcttcagca tctacgctca tcaagtgatt tagatgctct
     2281 taaaggatgg gctgctgtgg ataaagaaaa atgtataggc tgcggaaagt gtgaaaagcc
     2341 tgcacactgt accgctgtaa aaatcgagaa taaaaaatct gtaatagacc ctgagaagtg
     2401 cttgggctgc ggcatatgtg aggctctttg tcctacaaaa gctatatcaa tgcatattgc
     2461 agagtaagtc atgggaataa agtatcggaa tagagtgccg aaacaaggaa tcggagtaag
     2521 ttatcagagt aagaaatcgc aagaattttt acaataaata aataactata ataactataa
     2581 taataactat agcaactata gcgaaaatta catttttgag aaaaggaacc tatcttaaca
     2641 ccggaatggc ataccagaaa ggtataaagg atagggtcct ttttttataa aaacatcaaa
     2701 tataccatta taccattgaa ataattgcta gaaatatata gaatatagct gacacatgca
     2761 aatatatcac taataaacaa aagaaaatgt tagcaactta cataacttac aggaaaatgt
     2821 aacagcttaa cagatttggg gaaaatatac ctgccatata ttatgaagaa cctgttccag
     2881 aaataataaa atatttaaga agagagataa agcaatggga aaaataattt gttttggtga
     2941 aataatggca aggctaaacc cggagggata tctcaggtta ggtcaggcaa agaaacttga
     3001 aatctcatat ggaggtgcag aagcgaatgt ggcggtttct cttgcaaact atggcctgaa
     3061 ttctgggttt gttacaagat tgcccgaaaa cgatctttca aaagcggcag taaaggattt
     3121 aagaggatat aatgttgatg tttcgcatat tgtttatggc ggtgaaagac ttggaatata
     3181 ttttgtagaa aagggagcat ctcaaaggcc ttctaaggta atttacgaca ggaaatattt
     3241 ctctatagct gcagccggaa agcaggactt tgattggaat aaaatttttg atggagcttc
     3301 ttggtttcac tttaccggta taactccggc actttctgat ggtacagcag aaatttgtct
     3361 tgatgcatgt aaatgtgcaa aggaaaaggg cattacagta agctgtgatt taaattatag
     3421 aaaaaacctg tggtccagtg aaaaggctca aaaagttatg agtgagttaa ttaaatatgt
     3481 tgatttatgt atttgctgaa ggatttattg cataagcata acaaagcaaa tgaaagagat
     3541 cagcatcaaa aggatattct agaaagcaac aatatcttaa cagaaactaa cccggaaaaa
     3601 ataatgtggg aaacattgcc ggatggagat atgggtttaa gaacacctcc aggtggaggc
     3661 cccatttcag aagaacaaag atttagtgct ttaagtgatg gctcttttta ctgcgtttat
     3721 cgttccgttg atggacatcc ggtatacacc tatagccgtg acggaggtca cacttgggat
     3781 gtaccccaat ataaacgtta ttatgacgga cgtctcatga aacatcctag agctgcaaat
     3841 tttgcatgga agtgctccaa cggcaagtac ttatactggt atcataacca tggaggaaca
     3901 gggtatgagg atagaaaccc tgcctggata tgcggtggta ttgagcagga tagcccaaat
     3961 ggaagagtta taatatggac gcagcctgag atcctgttgt atgaggatga tacttttgtc
     4021 cgcatgagtt accctgatat aatcgaagag ggggataggt tatttattac agaaactaat
     4081 aaaaatgcag caagaataca tgagataccg gctaaatttc ttgaaaagct ttggaatcaa
     4141 tttgaaatat catatgttga agaggacggc ttgctatagg aagtaatact acagaaaccg
     4201 gactgcgtat tgaagatgtt gttcgaatta catcggatgg agttgaacag cgtgggcaaa
     4261 ggatcttgtt aatcttgaca aaaacctttc aaggttaaga gagttatttg gtacagaaaa
     4321 acctattgta gccggttgct atatgtggga ttatggtgga aagaatccga tgcctataga
     4381 tttaatgcaa tatcagcttg acaaatatta tgaatggctc aagtctgggg aaattgatgg
     4441 tattatcttc tgttccaact gcatagccga cctgggtctt gaagcagttg acattacccg
     4501 tgaatggatt aaaaaggtgg gcggagagcg gctttagcct atgaaacaat tatacgtatt
     4561 ttaccttgtt aaataaattt gcataaaatt ttcacctatt tggatgaatt ggtataatat
     4621 attgacgatt ttagccgata ttattattta cagtcatttt tgtttaaatg accagtgtaa
     4681 ttaacttaca ttcatctaat aaggggtgtt acaatgcgta aaagaaagtt gataattatt
     4741 ctaatttggc tgccgtttgt attaatagta cttcttttag gaggctattc cagcgaggtt
     4801 tacagtaatc gatcaaaggt agtagagggg ataattgacc taaaccaaag agagttagat
     4861 gaagaagtta ttaaactgga tggacaatgg gaattttact ggaatcagct gcttgaacct
     4921 ggcgaattaa aggagatagg tatacaaaga agcgggtaca tagatattcc aagttcatgg
     4981 aatcggtata taattaacga taaagaatta tctggagatg gttatgctac ctacaggtta
     5041 ctttttaaga cagataaaaa tgggagattg ggtataaaga taccaagaat attaacatct
     5101 tataatcttt gggttaatga agaacttata gctgctgccg gaatggttgg taaaagcaga
     5161 gaggcaatga cccctcagta tctcccgcaa gttgctttct ttgaagcaaa gcagggtaaa
     5221 aatgaaatcg taatacaggt atctaacttt tatcaccgta gtgggggaat attagaaagt
     5281 atattcatag gtaatgaaaa aaagattatt ggactgcgct ataaaggtat agcatgcgat
     5341 cttctattat ttggcagctt aacaattata ggattctatc atatcgcact atttctcttt
     5401 agaaaaaagg acacaacccc cctttatttt ggtttgttct gcctttttat tggaataagg
     5461 acattgttgg taggagaaag attcctcatc tatctatttc cggattttaa ttgggaaatt
     5521 gctcataaaa tccagacgct gactttctat cttggggtac cttcaatcct aatatttttt
     5581 aaaatggttt ttcttaatga ctttaatacc aagtttgtaa aaattatata tatggtaggg
     5641 attacgtttg gatgtctggt tcttctcact cctgcaaaat tttttacttt gcttaatcct
     5701 gtatatcaga tattttcatt tattgtaatt gtatacataa taagtacttt cattaagatt
     5761 cttctccgga aggaaaaggg catcacgtta attatagcag gtgctttggc cttaattctg
     5821 actagcctaa acgatattgt ttttttaagt atatggatga atgataacag atctccactt
     5881 ttgaggactt tgttcagaac tggtaacctt tcctctgttg gccaactaat ctttgtgttt
     5941 acaaactctt tagtattagc caaaaagttt tctaatgctc ttgaacagca agaagtgatg
     6001 acagctcagc tgaaagaagt aaacctcaat ctggatgaac tggtgatcaa acgaacaaag
     6061 gctctggaag agtcgaaggt aaaaatagaa tgccagaaac ttgaattgga gaatgcaaat
     6121 cgaaaactcc agatgctttc gttgaaagat ccattaaccg gcttagggaa cagacgatat
     6181 tatgacgaca ctatgcttat ggaatgggat caaggtttga gatgtaaaag acctgtttca
     6241 ttaatgatga tagatattga tttttttaaa tcatataatg attgttatgg tcatagtgca
     6301 ggagatgatt gcctgataca ggttgcgcag gcaattaagg atttcattaa aagtacaggc
     6361 gatctggcag tccgttatgg gggagaagaa tttgtagtaa tcatgccgga aacaggaaaa
     6421 cctgaagcta ttaaaaaagc acttttattg cggaaaacca ttgaagatct taatatccct
     6481 cacaaatgct cacctgtaag tccctgggtt acagccagca taggagtaac ttccatgatt
     6541 ccggacattg aatgttctcc aaatgaatta ttccttgcgg ttgataaggc cctttaccag
     6601 gcaaaagctg ctggcagaaa ccaagttaaa ttttcggcag aaacataaaa ttatggtatg
     6661 cattatgtga actaccccta cctacgctct cactccgtga gcaatgaagt taagaggttg
     6721 aagaattctt tccgaaaagg ttaaaagatt ttgtaaataa ccatatttta gttaaactgt
     6781 gattatagaa ttatgaaata ctcttctgaa acgtagtatt ctgttattta ttacttcaga
     6841 atgtaccctg ttggtgagaa gaatcatata tagactggta tttttgtcta taagaactga
     6901 tgtacctgaa acaggaaatg caagctctaa attacagttt ggaagaaaag gtgaatgttt
     6961 ctcttgccat aagtcttttt gcaggaagac aggtgtcttt agcgaaggct gcaaagcttg
     7021 caaataaatc attgacaagt tttatggata tattaaaaaa ggttaatatc ccatggtgtg
     7081 aatatacaga agattttaaa aatgcagatg atttcgcagt aaatctacta ttacaggaat
     7141 cggagagttg atataaaata tcggttattt caaattcaac ccctcatacc gtatttcttg
     7201 ttaagcgcgt ctaaaccgca tgcctggcta tctaatgcac tctgaactca aattttgtat
     7261 attagcaaaa aatgtgtatg tttacaatag tacttcattt atgctactgt tatttatgaa
     7321 aaatgcttaa ctgcacagat aacaggtaca ttaaatactg ggtttattaa ataccggaaa
     7381 tactgggttc attaaatacc ggcaatattg gattcattaa ataccggcaa taaatcagat
     7441 tatcagatta agtgaaagga tgatcacgtc ctatgaatga gaataatgca ggaaaagaca
     7501 taataaaaaa taataactat agtacatata caaatactga atcatatgat tcatatatta
     7561 atagtaacaa caaaagtcac agcgacaata acaacagcaa caataaaaac aataataaca
     7621 ataataacaa taataataga attatgattt ctcttaccag agaattaaag caaaattatc
     7681 aactatatat cttagtatta cctgcagtat tgtatttcct aatattttgt tacataccaa
     7741 tgtatggagc acagattgct tttaaggagt ttgtcccgca tcaggggatt ttagccagca
     7801 agtgggttgg attcagtcag tttgaaaggt tttttaattc ctaccagttt aaaacactat
     7861 tcagcaacac tcttctattg agtttatata atcttgttgc tggtttcccg gtaccaataa
     7921 tacttgctct tatgttaaac cagttgtctt caagtaaact gaaaaaagtt gtgcagacgg
     7981 ctacatatgc gccacacttt atttctgtcg cagcattgtg cggaatgatg gtagttatgc
     8041 tttcacctac aggcggcata ttttacaaga tattggaagc aatttttgga gaaaatttgc
     8101 ccatcccgtt aggtaaccca aggtggtttc gaactattta tgttgtttcc ggcatatggc
     8161 agcatgcagg ctgggaagcc ataatttata tagctgcact tacatctgta aatcctgatc
     8221 tatatgaagc tgcagacatt gatggagctg gaaaacttaa aaaaataatc tacatagact
     8281 ttccatctat aataccaacg gcaataactc ttttgattct aaatgccgga aaaattatga
     8341 gtataggctt cgaaaaggtt ttcctaatgc aaaacagcat gaatctggct acttcagaaa
     8401 ttatctcgac atatgtttat aaaataggta ttatcggaag cgaatttagt tattcaagtg
     8461 caatcggctt atttaattca ataattaatt taattttagt tattactatt aattatatct
     8521 caaaaaaata caaagaaacc agtttgtggt agggggtgca ctgatgaaaa gacaagcaac
     8581 tcaaaaagat aaaatattcg attttgtatt aaacataatt atggttgtaa ttctgattat
     8641 tactttatat ccattatact ttgtagtgat agcatccata agtaatccgg actttacata
     8701 tgcagggaag gttattttgt taccgaaggg cataactttt gcaggctttg agataatact
     8761 ggcagacgga cgaatatggg aaagatatgc aaatacaatc atatatacta ttgtaggtac
     8821 tgcagttaat ctaattatta cattacctgc ttcatatgca ttatcacgta aatcattcaa
     8881 agcaaaaaaa tatataaatc tttttatatt atttacaatg tttttctcag gagggcttat
     8941 tccaacttat cttttagtca aaggcctgaa gttgatagat agtatgtggg ctattatcct
     9001 tgtatcagca gtatcggtct ggaatttgat tattgcgaga acatattttt cagggaatat
     9061 acctgatgaa cttgaagagt ccgccttgct ggatggctgt tcccatataa agtttttctt
     9121 tctggtagca ttgccgttat caaaagtgat tgtaatagtc cttgcactgt tttacggaat
     9181 taatcactgg aatggttatt ttaatgcttt aatatatctc aacacacctc agaagtttcc
     9241 actgcagtta tttctaagag aaatacttat tcagcagcaa agtatggcta tggatcccga
     9301 actggcaaga gacatgcgat acactgcaaa cttgataaag tatacaatga ttatcgttgc
     9361 aacgggacct gtactgatta tatatccatt tctacaaaag tttttcgtga agggcgttat
     9421 gataggagca gttaaggggt aagtgcgatg cagttaaggg gtaagtacag taagaatatt
     9481 attgcttgaa aaagcaaaat agtataaatt ataaaatagg aggatgtaaa tatgtatatt
     9541 gtcaaaaaaa ggttttacaa aaaggcagct ctaatggcac tggtattatg tatactgctt
     9601 acagctacag catgtacagt taacaacaaa gcatcgacga attcatcaaa tgaaagtaat
     9661 gatgctacca caacaaaaca gcaacaatcc aattttaatg ctacagggta tcctgtggtg
     9721 aatgaacctg taactataaa aattgttgca aagcacagta ttggcaaaga tcagtacaaa
     9781 tatggtgaaa tgcagctttt taaggaaata tctgagaaaa ccaatgtact tgtagaatgg
     9841 gatgaaatag ctggtagtgc atttgccgaa aagaaaagtc tgatatttgc cagcaatgat
     9901 cttcctgacg ctttttgggg agaaggaatg tctgactcgg atattttaac taataaggat
     9961 tttttcatac cgcttaatga ccttattgat aaatacggag tcaatataaa aaaggttttc
    10021 gcaaaaaggc ctgaaaccaa acgttcaatc atagcttccg atggcaatat gtactccctg
    10081 tttagagtaa gagaattgta cttcccagct ggaagaacaa catggggaat aaacaagaaa
    10141 tggctggata aacttggcct ggaagtaccc aaaactacag atgaattcta cgatgttcta
    10201 aaagcattta aaacgcagga tccaaatgga aacaagaagc aggatgaaat tccctttata
    10261 ttccatcatg caatgggttc aggttcagta agaagtgctg cagattttta tcctgcattt
    10321 ggcgtgtatg agaacacttc caactctgac cttacatacc accttatgtt aagaaatggc
    10381 aaagtagtat ttactcccat ggatccaggc tataaagaag ccttgaaata tttaaacaag
    10441 ctttacgctg aaggactttt ggatagggaa gtattcacaa ctgattcaag tacatattat
    10501 gcaaaaacca gaaatgaaga ggatctggta ggagtaatca ttgattggac aattgatagc
    10561 gcagttggtg ttgatagagc aaagaatgat tttgtacagc ttggtgcatt aattggtcct
    10621 aacggtgatc agggatgggg tattgtatca ggttctcaga tcggcaggca tatgtttgaa
    10681 ataacatcta aaaaccaata ccctgaagca accatgagat gggtagatga gttttatgat
    10741 cccataaact ctgtacaggc tttctttgga ccaattggtg agatgataaa gaaagaagga
    10801 gaaaaactca ttgctcttga accggaagaa ggagtaagct atggctcatg gaaatgggga
    10861 cgcacttgtg cagacagtgg tgcatatgct gctcttaagg aatacagctc tttctttatt
    10921 ccaccggaac aacaggtagc acgcgcaaaa gcagaagaat acgttgcaga acatctgcag
    10981 aaggaacctt tccccaatat attcttctct acagaggatg ttgcagcctt aaagaaaata
    11041 ctagtcgaca taaatgcatt tgttgaaaca tcaagagctc agtttgttac agaaggaaat
    11101 atagacaaaa aatgggatga gtatgttcag cagcttgaaa aaatgaaaat taatgatgca
    11161 ttaaccatac ttcaaagagc atatgaacag tatattaaaa acaattaacc ttttagcagc
    11221 catgcagcaa ttttagactt gaatggatta ttagacttaa atggaatatt agacttgaat
    11281 gaattatcgg gcggggagca atctccgccc ggcataaata ctgaagataa atgaattaca
    11341 gctactgggc gcattttgtt tatttgaaga aaatattggt aaggggcata agtggaggat
    11401 gctatagagc cttttaatca gagcgaagga atagcaaaat cagtgtttga gcaaaataac
    11461 tacaactatt aaactattat ttgaccaggt tattttgtga gtttgatttt gcccgtagct
    11521 gaatttaaag gctctaagca ccgtaacgat agcccatggc aaatatttct tcaaatttta
    11581 aatatgcacc cttgctgcca tagctatctt gaattataaa atgcaggcag ctataataaa
    11641 tctaatgatt cagcaacgta atgatgcagc agctatgata aatatagtga cacagcagct
    11701 ataataaata taatgataca gcagctataa taatgcagct ataataaatg taaatgaaat
    11761 ggcaatgagg gtaggaggag ggtatgacat gaaagcaata cagaaaaaag cttttttata
    11821 caggtatatt atatccttct tacttatttt aattgttcca atggtctgtc tattattcta
    11881 tattgcaaat ttgataaaat ccaatatgca aaaggattta atgtctgctg attataacaa
    11941 tacagtttat atacataact ctgtagataa cagtatccgc aagtttgaaa accttgcgct
    12001 gctgacaatg agcaattcca atttaaaaac ttactttctt gatcaatcat attttaacta
    12061 ttatcaggca gttagtacac ttaagtatat taagggtaca aatgagcttt tgaatgtgtt
    12121 ggtattatat aacaaatcaa atgatttggt tatttcaaat tttggtacta taccaaagca
    12181 gtatttcgga aacaaagtac tttcggcaac tcttgataat aaaattgctc tgaaagattt
    12241 tattgaaact gtcatacagc cggtatatac atacgggtat gtaaatattt ccggtatagg
    12301 agaacggagg tgtttgctgt acttttaccc cactacatct gattttgaac aatcaaaatc
    12361 tattataatg atgattgttg atgttcaaaa tataattgac ttatttgatg aacaatataa
    12421 tagtgttagt ggttctgtat ttattctgga tgagagtaat aataccgttg tacaatatgg
    12481 aaacgcaaac gaaaaaatcc tgaataatta tttacagtat aacaatagac tcttgaatgg
    12541 atcggaagta tttttgtatc aaaataataa atattgtgtt tccggtataa cttcattaaa
    12601 aactggatgg aaatacatta gtattgtatc attttctatt gcaaatagag aatttacata
    12661 caatatgtat attttctatt tcgtgtttat tattttgttt ataataagtg gattaggcat
    12721 atatatttca ataagactta attacaaccc tctgcttaaa ttaaaaaaac agatagaacc
    12781 aattgttgaa accaaatcat tgaatgaggt tgagacaatt cgtactgcag taaacatgct
    12841 tatcaataat gtagatagaa tgtcttcatg gatgaaagac attcagcctg ccgtacataa
    12901 ctatcttata cagcaaatgc ttttcggaca taaaacagat attgatgatt tcaaacaatt
    12961 aagtaaggaa atttgcttta gcttttataa aagcagattt atggttggca taataaagct
    13021 aaaaagggct gattatgaag tagtaatatc agatcttatg aaaattagta atgacaatat
    13081 tacagtatat tatctcaatt ttcctgtgga tgatatgctg ttagctgtat ttaatttgga
    13141 agaacatgat tatgaaaaag ttatagcttt cttaaatgat tttgtagaac atataaacat
    13201 gaaaaacaag tatgatatac atatatcact tggcagacta aatgagcttc aagacgttag
    13261 taaatcttat tttgaggctt gcataggcga gctgttcaaa aaatttggca gccaaaatca
    13321 gagagtatat tgttttaatg atataatgaa gagtactgaa agtccgtcaa cgggattttg
    13381 tcccggtcat ataagagatt ttgagttgaa catattgcgc gcaaatatag aatatatagc
    13441 taaatttcct tcaataataa tgcaggagat ttctacaaat tatttaccag aatattacat
    13501 agattatgtt atatatgaac tgataagggt tgttataagc agcttggcag gcgatgcaag
    13561 ccaggaagta ctccttgaca ggatatggga ctatattttt gccaggagcc ccatatcttc
    13621 aaccaatgtg cttgaagatg tcatttcgtt tatatgtaat accattattc aacatattcg
    13681 taaaaatcca ataactaaag attctacatt aaatgaaatt ttgaaatata ttgatgaaaa
    13741 ttattataaa ccggactttt ccattcagtt gacagcaagt gcatttaaca tgtcaccatc
    13801 atggctaagt cattatttta agcaaaatat gtatattact atagctgaat ttgtccagaa
    13861 taaaagaata aaatactcac aacagcttat tcttgaaaat gagtgttcga caaatgaaat
    13921 ctctgaacaa ttaggatatt gtaatgtatc aagttttatt agggctttta aaaaaacagt
    13981 aggaatgaca cccaaacaat ataaggaaac atttggcaac agtaaaccgt aaggttcggg
    14041 tgtgtataat caaatttatt tttgatatgc cacgaaagga attgttatga atagaaaaat
    14101 tttaccaatt gaagtaaatg tttgtgataa attctggagc acgataatag aaaggaacag
    14161 tgaagtgaca gttcctgtgt gccttaaaag atgcgaggaa acaggaagga tctctaattt
    14221 cgcaaatgct gcactcgata aaaaaggcag ctttcaaggc agattttata acgatgcctg
    14281 cttgtataag gtgatagaag gagtagcaaa taccctcaaa ctaaatccaa atcccgtatt
    14341 ggaagaaacg gctgacaata ttataaataa gattgctgcc gctcaaatga acgatggata
    14401 tattatgact tattttcagc ttcaagaacc ggagaatcgc tggaccgata tgggaatgca
    14461 tgaaatgtat tgtgcaggac atctacttga agcggctatt gcctattacg aggcaacaag
    14521 aaaagttaaa cttttatgtg ttgcgatcca gtttatagaa catataatgt gtttgttcgg
    14581 cgaaggcaaa aggcattggg tcccggggca tgaagaaatt gaacttgctc ttgtaaaatt
    14641 atacgataaa accggggaga agaaatacct tgagttctca aagtggctga ttgatcagcg
    14701 tggaaagggc ttgtcaaggg ggagagacta tagtcaggaa ttttataaca ctaaatacaa
    14761 tcaggatgac ataccatttg tttcgcaaag aattgcaaaa gggcatgctg ttagagcaat
    14821 gtatctttat agtgccgcag cagatattgc atatgaaacc ggtgataata gttatatttc
    14881 cgctctttta ggcatttggg ataatgtagt tcagaaaaat atgtatataa caggtggtat
    14941 cggttctact aaatttaatg aaggtttcag cacagactat aatttgccca ataaaacagc
    15001 ttattgtgaa acatgcgctt ctgtgggaat ggtattatgg aactatagaa tgtggcgtct
    15061 cactgaggaa gcaaagtatt ttgatattgc agagcgcgct atgtataatg gcgttctgtc
    15121 tggtgtaagc ataaccggcg atctgttctt ttatgataat gtattggaaa gcgatggaga
    15181 taaaaaaaga agtccctggt ttacgacttc atgttgtcct actcaaattg cacgtttcat
    15241 acccacagtg ggcagctatt tctatatgaa taataataat tgtttgtgga taaatatgta
    15301 tggttcttct caagcgaaaa ttgagataga taaacaagat ataatagtca ttcaaaaaac
    15361 tgattatcca tatgatggca aagtagaaat aatatttcat acaaaaaata agttttcagc
    15421 aaaagtcaaa ttgcgtattc caggatggtg tgataattgg cgcttgcatg tgaattcagc
    15481 aattaaagat gctgctatgg ataaagggta cctcactctt gatagagaat ggtctgatgg
    15541 ggatgttatt actttagcac tggatatgcc tgttaaacta aactacagtc atgaatgcgt
    15601 tgaagaaaac aaaggcaagg ctgcaatttc cagggggcct cttgtgtatt gcattgaaga
    15661 gattgacaat atgcaatatg atgatgtaaa aattgaccct catggtgaat tctctgatat
    15721 cagcgcaact ataaatactg cagataatat aaatactgaa gatcccgcta atgatgtaaa
    15781 tacagtaaat tctgcagaaa caagatttat ccgatgggat gttcaaaata tagatgctgt
    15841 agctgtacct taccatttat ggaacaaccg gggacagggg aaaatgaagg tatggattcc
    15901 tcttattcca tcatgttgat gtcaagtggg tgttcgggta aaattcgcat aactaataaa
    15961 aggagcggtc tttatgtatg aaaaaatata tagtaagctg ccactgggtc aagtattggc
    16021 tgatggctgg ctaaaagtgc agcttaacat gcaggcaaaa ggacttacgg gaaatataga
    16081 gtcaatatgg agtgatcttg gcccggatag cggatggctg ggtggaaatg gagagagttg
    16141 ggagcgaggc ccttattatt gcgatggact tgtgccgctt gcttttcttt tgaaagataa
    16201 tgttttgctg gaaaaagtgg agaaatggat tggctggaca ctacaaagta aaaaagcaga
    16261 tggatttttt ggcccgggaa gtaatgatga ctggtggcca aggatggtta tgctaaaagc
    16321 tttgatgcag tattatgaag caaagccaac tcctgatatt ctatccctaa tgtcagatta
    16381 ttttcagtat cagcttaata aaattgatga taaaccgctg actgactggg gaaaatttag
    16441 agcatcagaa aatattgatg ctattctatg gctttatgaa aaaactaata aatcaaacct
    16501 tttgaatttg gccgacaaaa tattcaaaca ggctttcaat tggtctgact tctttaagga
    16561 ttttccttat gagaaaccaa taggaaatta tatgaattgg caggaagtac atgaagccca
    16621 tcttcctgta aatgaaagaa ctatgcgtta tccggattat atgaaaagtc atatagttaa
    16681 tgtagcaatg gcagttaaat acccggcttt ggccatgcgc ctgtataacg atcaggaaca
    16741 aaaggaaatt ttaaaaaaag gccttgaaga tttgtataaa taccatggag tggcaaatgg
    16801 aatgtttatc ggagatgaac accttaacgg aaataatcca tccaatggca gtgagctttg
    16861 tgctgtcgtg gaattaatgt atagcctgga gaaagttttg gaggttatag gagacacatt
    16921 ttatgcggat ttgctggaaa aagtcacctt taacgcttta ccggcgacaa tcgataagga
    16981 attctgttca catcagtatt tacaacaggc aaatcaggtt ttatgcacca ttgcaaaaag
    17041 agattggtat aacaatttag atgactcaaa tatttttgga ctggaaccta attttggatg
    17101 ctgtactgca aatatgcacc agggatggcc taagtttgtg caaaatatgt ggctaaaaac
    17161 taatcgtggt tttgctgcca tgctttatgg tccatgcaca ctaataacag aacttaatga
    17221 tagaaacaag attaaaatcc atgagaagac taattatcct ttcgaaggaa atatagattt
    17281 taatatctgc atggaagctc ctgaaacttt cgaattgcaa ttgaggattc cgggatggtg
    17341 taaaacattt tccttgtttg taaacagcag caaggtttcc tatacaatta gagatgggtt
    17401 tatacatata gaaagattat ggaaagatgg agactgtgtt aaacttattt tagatatgcc
    17461 aattataaaa tcattttggg caaataattc tgtttctata gagagaggtc cattactttt
    17521 tgctctagat ttggaggaaa ggaaaaagct gataaaccga aagccgcctt ttgcagattg
    17581 ggagctgtat cctcataaga aatggaacta tagattgctt cttaataata tgaacgttaa
    17641 agaaaacttt aataatttaa atatacatga taataaagat gaacaaagcg aaagctcgaa
    17701 cccatttaaa gaaaaaaata aattcgttga gatagaagta tatgctgctc cttgcgaaga
    17761 atggaaaatt gtcagaaata gtgcagatct tcctccagaa agtcctgctg ctgtagattc
    17821 tgataattgt attaagctga gattagtacc ttatggatct acaatgctca gaatttccca
    17881 attcccatgg ggtgatatac aatagctgcg atacaagcaa ggacagtaaa acagttaaat
    17941 attagaggta tgcaagagca aatttattta gcacatagca ggaatgtagc aggaatgtag
    18001 caggaatgta gcaggaatgg agcgtaaaat gaaaatttat ttaattgacc tagacggcac
    18061 attatatagg ggaaatgaac ctattgaata cgctgctgaa tttatagatt atttgaaccg
    18121 caataacaga aaataccttc tgacaacaaa ttgcccatta aatggccctg aggaaattgt
    18181 tatgaaatta aaaacaatgg gcatagctgc aacaacagat aaagttctta caagtggaat
    18241 ggcctgcaga gattatttat tagagcatta ttttggaaag aatgtttttg ttataggttc
    18301 accaagtttc aggaaattgc tttgcaattc gggtattcaa ttaacagatg aatattctga
    18361 tgtagtggtg ataggttatg atcaacagac aacttattca caaattaaac aggcatgcat
    18421 aaatatacta aatggtgctg aatttatttc aacaaatatg gacaatgtaa ttccatttgg
    18481 aaaaacgttc atccctcaca caggagcaat tacatctgca gtgggatatg ctgtaaataa
    18541 ggagcctgtt gttattggaa aaccaaatca acatatgttt aacagtgctg tgaaaatatt
    18601 aggttgcaat agagaggatt gcattgttat aggtgataga ctggatattg atatacaatt
    18661 ttcttataac aatggtatcc ctgggtattt ggtttttacc ggggtggcaa aatgtactga
    18721 tgtggaaaat agtattatca aacctgacaa atgttttaat aatttaagag atattataga
    18781 gatggagagg agaaattttg atgttttata atccgtatga attacttaaa atccaaa
//
LOCUS       DAOSDD010000064        18728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1214_length_18790_cov_5.542514, whole genome shotgun sequence.
ACCESSION   DAOSDD010000064 DAOSDD010000000
VERSION     DAOSDD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18728
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_18790_cov_5.542514"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..978)
                     /locus_tag="PK733_07610"
     CDS             complement(199..978)
                     /locus_tag="PK733_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOJ10442.1"
                     /translation="MEYIGKNLLNNDSLAGKDVNIAKQDTKLNLEDKLRELILGLGGS
                     FVGFSDVSKMLPESLKKYPYAISFGIRLSDAIIDEIEDKPTFTYFNHYRSVNALIDQI
                     GLRIMLFLQDNGYKAYTIAASQSIPDAPEPFSGVFPHKTGAVASGQGWIGKNGLFIHR
                     EFGPRVRLGTVLTDSSNSNLGDMECSEGNIILKSRCSSCNRCVEACPAMALTGKEWHI
                     GMKREEIIDARACSEYMNKNFKHIGRGSVCGICIKVCPCSK"
     gene            complement(1028..2929)
                     /locus_tag="PK733_07615"
     CDS             complement(1028..2929)
                     /locus_tag="PK733_07615"
                     /inference="COORDINATES: protein motif:HMM:NF037720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOJ10443.1"
                     /translation="MKLHFSGDLQECMKGIGILAGDFGYEISNNGKDCLNIKVEKRSG
                     CDIYVAKKGLDAVIRYSEKIHFFRALGLLLEALKHNDEDFEITEEPQFTMNGAMFDVS
                     QGNAVIGVETVKNFLRRMAVMGLNMLMLYCEDSYEVKEQPYFGYMRGKYSYEDLKECD
                     DYADIFGIEMIPCIQTLAHLIDILKWPVYWDMRDDEETLLVGEEKVYKFIEDLIVAAA
                     APFRTKRIPIGMDEAWRLGMGAYFDKHGLRKKFDIMNEHLARVLEIVRKHNLKPMFWS
                     DMYFRAASKSGGYYDIDIEISQELLDTVPKGAQLVYWDYYHDDEEFYREFIKLHRKFG
                     SEPIFAGGIWTWIGFCANWGKTFRTTNPALNACKKEGVKEVFVTIWGDNGTESNVYSN
                     LLGLQLFAEHGYSKELDMDKLKNRFEFCTGCNYDDFISIKYLDEIPGTDEDNMETCNP
                     SKYLMWQDLLTGLIDYNIEGLPLNAHYEKLAKTLENAVNRNGEYGFIFEYLSKVSQVL
                     SIKSEMGIKITEAYRNGDSSALRAFVEIQLPELEKRVKELRSIHKEQWFVINKALGWD
                     IMDMRYGSLLIRIQSAIEQIGDYLDGKFERIEELDEERLSFTGKPGLVKYGNFYGFIV
                     SPSRIAPRG"
     gene            complement(3484..3999)
                     /locus_tag="PK733_07620"
     CDS             complement(3484..3999)
                     /locus_tag="PK733_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10444.1"
                     /translation="MVHYTNYKGDRYYLHEGISKKGNPIYWFSKSTMGKGKLAEKIPN
                     GFEIYEEPNGMVYLWKNLPQIITVNEINIVQNSISKELDAKVYVKKNVITIYLFEGEC
                     VGYQALMRFILIDEKTRKFEAQRYCFRGSIDSWIGLDTNTDLKKLAKKCCHHLGKDSF
                     YDLPYMVDGFL"
     gene            4374..4964
                     /locus_tag="PK733_07625"
     CDS             4374..4964
                     /locus_tag="PK733_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOJ10445.1"
                     /translation="MEIISKYEDYEGLRYTYKDYLQWPEDERWELIDGVPYSMSPAPS
                     KRHQEICLALCSEFYNYLKGEPCKAYIAPFDVRLVDEGESDDDASTVLQPDIVVICDR
                     TKLDKAGCKGTPDLVVEITSPYTAKKDVIRKFAAYEKFGVKEYWIVRPEEETVTVFKL
                     SNENKYGRPEIYCRNDKIQVGIFDDLIIQLEDIFKD"
     gene            complement(4961..5137)
                     /locus_tag="PK733_07630"
     CDS             complement(4961..5137)
                     /locus_tag="PK733_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10446.1"
                     /translation="MNFSEFLFAEKMKKAQKLICDSSNKIKIQDIAEKLVISPTPKKY
                     ITKMNFTSGNEDLL"
     gene            5715..6788
                     /locus_tag="PK733_07635"
     CDS             5715..6788
                     /locus_tag="PK733_07635"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ10447.1"
                     /translation="MKMLVTGGCGFIGSHIVDMLIERGHDVWVIDNLSSGKLDYLNTK
                     AKFYKMDITQSSINEIFEKIHFDMIFHEASMHNASNSEKDPTLDANINIMGTLNILNN
                     MRKFNINKIIYASSTAVYGNVQQLPIKESYQGKPASCYGLSKYFTEYYIRLYSHLYDI
                     DYSILRYSNVYGERQNDSEECNVISSFLYDFKLGRQPVIFGDGQQTRDFIYIKDVVSA
                     NFEAITKGARNIYNICTSTSATINYLYNILRDIFMTSHESHEPYELYKPYKSYDLHQL
                     HKLYEPLYNKGQKEDIKESSFDNTKAIQDLDWIPKYSLREGLEKMLFNMDGQVQSDRQ
                     TQSREHILSRSGYLQTLSRNVPI"
     gene            complement(6932..8263)
                     /locus_tag="PK733_07640"
     CDS             complement(6932..8263)
                     /locus_tag="PK733_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ10448.1"
                     /translation="MQINGAVGNVQLMQKINRLKVLNYIRQHSEAARPEIAKSTGLSP
                     SSVTNIVTYLMDKKLVIEVGRVDSKEVGRKAALIRFNPSAASIISINIEISRITMALT
                     DLDGNIISKNEVRLYKQETNDEVLKIIEKGVSYLLQGEKRSLLSSNIIGIGIAVSGLV
                     LDDGRVEISTSLRWKGLSLKEYFEKIFHIPIYIQNNSKTKALAVLRKNGIHSDENIIF
                     LDLAMGVGIINFYRNEINEAVIGEIGHTTVKKDGPRCFCGNRGCLEVMCSVETIISQC
                     SELLKEGKCAELQKILNRNSMEEKSVSQTINNSKNDNTKIESDVNNTHISNTTNKNEL
                     LTYDMVLEAFEMGDKDVREVLRECGEYLGIGIANIINIFNPKKIIINGDVLLCSDYIY
                     ENALVEARSRAYERFMDGLEFNKVNIDMEKAIKGVSLYVTDRVFDLAGPEI"
     gene            complement(8455..9957)
                     /locus_tag="PK733_07645"
     CDS             complement(8455..9957)
                     /locus_tag="PK733_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ10449.1"
                     /translation="MYKVFIADDESMVVKSLKASIKWSEYGFEIVGEAYNGIEAYNEI
                     TSLKPDIVFTDIRMPGINGLELIKKVREENINTCFVVISGYAEFAYVQKALNYGALGF
                     CLKPFDENEIIGVLNKAKDVIKKSRILIEAELLTIIDDNSSFGRDRKAKILDILGVEK
                     IKETGIQALVTIGCNTICIPEINSISLRIGKNKNAYILSGSNSPESIKGYLARYPGMH
                     GDRDPKSVGNSGNLAEALRNNTLQKSLIGAGLSGVYYSSDHLHDIIDEASVAAYQYFM
                     TGDSDVYQYTEPEQDELGFVLMQLQNSIVKEEAALIQDAFSMVEKVFAEGAYNIKHAF
                     QVYNIVTHSFYSHDSVKSGEYIFDYEQLTNSYKNFTEMLENLSRQLVENAGIVQELTA
                     QDITNETFRPILQYVNENYCKDTSLSDISEIFTVNPSYISQLFKKQTGITFTEYLTNL
                     RVGHACNLLKSTCFSINEIAEKAGFNDYYYFTRVFKKVTGMTPTHYRNKG"
     gene            complement(9950..11773)
                     /locus_tag="PK733_07650"
     CDS             complement(9950..11773)
                     /locus_tag="PK733_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ10450.1"
                     /translation="MILNFLKKMQIKVQLIILAVSAGIVVFTILVVAYFQIADFIKKD
                     SYEYMTEMVFQIKKNISTNIDILDKLLNNVAYNSSVQEFMMEKDPYRHYKLFQQVDSI
                     CTNLQEIKNDIYDIVIISNIGEKYQLRGYNEKLNEIIKNIPVEESIYYTGTYLIRYPN
                     LPDFDLNCFIVITNVYSVSMDKSPGEKIGIAALIINKNMLGFETNKEDIKSSARFYLT
                     DRNDNLYSSNDNNNSIFDNIANTFENNKHKQINNRTIIINNESFVVYGENIDRIDGRI
                     ISAAPVSELFRDIRWVQQMTIVLFVIAIILLSIPFMLIISNILNPLNEFVNHIGSIRK
                     GNLKELKQRVSLEGYEEIEIMAGEFNALLDEIDNLTHRLVKTSTKLYEAELEKKKAEL
                     EYLKSQINPHFLYNTLEVMKGCAVDENAFKTLEIAKCLGQIFRYSVKGADIVTLREEM
                     EIAKSYIQIQQVRFNERFNVFYKYDDDVLNCNVPKMILQPIIENAVFHGLETKLEKGN
                     LYIEAEINKSDDLNIWVMDDGSGMDEETLCVIRQRLEGDTGERENKDDITTKTIGIGI
                     TNVNNRLRLTYGENYGVSIDSREGEGTGVLLKIPARRDMFV"
     gene            complement(12188..13837)
                     /locus_tag="PK733_07655"
     CDS             complement(12188..13837)
                     /locus_tag="PK733_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10451.1"
                     /translation="MKNLKKLVAILLAVVMVMSFFIGCNKKDKANKDNKTTTGDNTTK
                     QPEDPLKEHVEISLAYWGAGQALPEDVKEDAVRDALYKKLNITLKPVNLTWDDYRQKI
                     QIWAASSQLPDLFAIDAISTPNYEKWITEGIVHELPNNFSAYPSLKEIMDQPDIQAFR
                     YPLADPNGKFYCIPRPTYPNADMWANDLTVLMRKDWMENVGITKQPENMDEFITLMKA
                     FVENDPDKNGKKDTVGLTAYSASWLTALMLSYEPGVATGANYWVRDEKSGGWLPGFMT
                     ENCLEGLKELKRLYDSGGLDKDFATIKGTEGQDKFVTGRAGAYAHTGYPTTLSALNNK
                     FVKTFPDFKFDEAVTYLKPWSAADGNKYRLVAPTPWSESYINAKADDVKVDRILRLFD
                     YLFSEEGFNLIRLGIEGTDWKKDGDKIVITREKNESGSYDPLNKKYPITGLGSLSSWA
                     QDFSYKNPAVPEGILKMGNEELDWRIKNCKPINTDLRAGFIDYASKDKHVEQFADDMV
                     KAILSDDVEKTWNQIIKEHRANGYDEVIKDFNAKAAELGIK"
     gene            complement(13962..14861)
                     /locus_tag="PK733_07660"
     CDS             complement(13962..14861)
                     /locus_tag="PK733_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10452.1"
                     /translation="MSNNLENKLIKRTTGFDVFNYILMAVMAIITIFPFYYVIIISLA
                     DYAEVQKQLIYILPTSINFESYKLILQEKIVHSAFFVSVFVTLVGTLVSMCLSTAAAY
                     TLSKKDMPGNKIFFTMILIPMFFSGGLIPYYLTVQKVGLINKIWVMIIPHAINTFYLI
                     LLKNFFQELPPSIEESAKIDGANDIYILYKIVLPMSAPVIATVSLFYSVDKWNEYYTA
                     LLFISSRKLYPLQLVLREIIMNFTQLMASSIGAAIMESQKTVYSQSLQMAVIVVATVP
                     ILLVYPFLQKHFTKGIMFGAVKE"
     gene            complement(14948..15925)
                     /locus_tag="PK733_07665"
     CDS             complement(14948..15925)
                     /locus_tag="PK733_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10453.1"
                     /translation="MDSIVQREKNNLEKINKLNNSKNSLWKRMSYYKYFYLLILPAVI
                     FYIIFSYMPMYGVVLAFKNFSYSKGILRSPWAARHGFEHFYRIFSDAAFKKAFANTLI
                     ISVQRIIFEFPVPIILALLINEIGKSKYKRFVQTVLTFPHFVSWIIVTGIFFNILSDQ
                     GVLNQIIVAFGGEKVSILTQKSTFRALLYVTNNWKEAGWGTIIYLAAISGVSIELYEA
                     AIVDGAKRFQLVKYVTWPAIRSTIGIMLILAIANIMNAGFDQIFNMYNAAVYDVADII
                     DTLIYRRTFIIGADFSSSTAIGLFKSVVNFIMLFTGNAIVKKMSGRGIY"
     gene            complement(16127..17305)
                     /locus_tag="PK733_07670"
     CDS             complement(16127..17305)
                     /locus_tag="PK733_07670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10454.1"
                     /translation="MEGPRAAKVSELPLVERLSNEVFRTYDDDGQTMFQEFPDLFCEE
                     NIDNVRVIFEDGKPVSNMNYVIRPVSIYGCSINAASLGAVATLKDYRGHGYASSLLDD
                     CVSRMYNDGVDVLIISGDRRLYRNIGSTQAGLMYMYQIKDNDISKIDFNEYNIRELNI
                     KECSQDDLYNLANLYKQENVRFVRYYDKFYELLDSRKFMRKKTAQKKVVVVESKEGLE
                     AYFYMVVEGNEGTLYDCAGSRELIIKACKILLEKYDLKSINGRLMTYHTSAINYCREN
                     NIPLEERRLIGTIKIVNFKSLMESLRQYFYEIYDNKFINELEFENDEKGACFKFREEK
                     CIISDKEKLNDLIFGGAKVTGEDFVSNSDSTSNYEVFAEFFERCLPIPFIDPLSLNYI
                     "
     gene            17564..18439
                     /locus_tag="PK733_07675"
     CDS             17564..18439
                     /locus_tag="PK733_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10455.1"
                     /translation="MLKIDIHVHTRMQKGVERLGGGDYASPEELREMYKKLGIGKAVI
                     LPGINPECMNRIQSNEEAYILTVEYPELFYWFCNIDPRMGNNSPGSDLSHHINYYKKL
                     GAKGIGEICSNLYFDDPLVENLFFHSENCNMPVLFHIGPQIGGCYGLVDEVGLPRLEK
                     ELKKFPNLHFIGHSQPFWAEISSDVNQNNRNSYPDGKVTPGRVVELMRNYPNLYGDLS
                     AGSGYNAVSRDPEFGYTFIEEFQDKLYFGTDICDPRNDMKLSFWLDEALEKDKISKTA
                     YEKVCFKNALKLLEN"
BASE COUNT         5912 a         3583 c         2715 g         6518 t
ORIGIN      
        1 tccaacaaaa tttcgatagc aacggaaaga tgccgtgcgg ggaaatgtct ctaaagcagg
       61 tcttgcaaca ctttccttag gaagtattcc ttagtggtca gcaaaatttt gtttacatat
      121 tggacttttg cagtgtaata gagttatttc agcagaatta taattatatt tgtgaaaatt
      181 atttcattta tattattctc attttgagca agggcatact ttaatacaaa taccacaaac
      241 cgaaccgcgg ccaatatgct taaaattctt gttcatgtat tcagaacaag ctcttgcatc
      301 aataatttct tcccgcttca tgccaatatg ccattccttt ccggtcaaag ccatggcagg
      361 acaagcttcc acacatctgt tacatgagga acatctactt ttcagtatta tgtttccctc
      421 agaacactcc atatcaccta aattcgaatt agaggaatca gtgagaactg tccccaatct
      481 aaccctgggg ccaaactccc tatgaataaa aagtccattc ttcccaatcc agccctgacc
      541 tgaagctaca gccccggttt tatggggaaa gactccactg aaaggttcag gtgcatcagg
      601 tatgctctgg gaggctgcaa tagtgtaagc tttataccca ttatcctgca aaaaaagcat
      661 gatcctcagg ccaatctgat ctatcagagc atttacagaa cggtaatgat taaaataggt
      721 aaacgttggt ttatcttcta tttcatcgat aatggcatct gataacctta tgccgaaaga
      781 tatagcataa gggtattttt ttaatgattc aggcagcatt tttgaaacat cagagaaacc
      841 tacaaatgaa cccccaagcc ctaatattaa ttctcgcaat ttatcttcca gatttagttt
      901 tgtgtcttgc tttgctatat ttacatcctt acctgctaaa ctgtcattgt ttagcaggtt
      961 tttaccaata tattccatta tagttattat cccttacctt tctctggatt ccatactgcg
     1021 atttcgtcta ccctcttggt gcaatacggc taggtgatac gataaatcca tagaaattcc
     1081 cgtatttcac cagtccgggt ttgcctgtaa agcttaacct ttcttcatca agttcttcta
     1141 tacgctcaaa ttttccgtcc agataatccc caatctgctc aattgcactc tgaatgcgaa
     1201 taagaagaga accgtaacgc atatccataa tatcccatcc aagagccttg tttataacaa
     1261 accactgctc cttatgaatg ctgcgtagtt ctttaaccct cttttccagt tccggcaatt
     1321 gaatctcaac aaaagccctt aatgcagaac tgtccccatt acgataagct tcggttattt
     1381 ttatacccat ttccgactta attgacagca cctggcttac tttagacaga tactcaaata
     1441 taaacccata ttcaccattc cgattaacag cgttttccag ggtcttcgcc agtttttcat
     1501 aatgtgcgtt aagcggcagt ccttctatat tataatcaat caaacctgta agcaagtcct
     1561 gccacataag atatttgctt gggttgcagg tttccatatt atcttcatct gttcccggga
     1621 tttcatcaag atatttaata gaaataaaat catcataatt gcacccggtg cagaactcaa
     1681 atctattctt taatttgtcc atatccagtt cttttgagta tccatgttct gcgaacaact
     1741 gcagtcccag aagattggaa tagacattgc tttcagtacc attatctccc catatagtaa
     1801 cgaatacttc cttgactccc tctttcttgc atgcattcaa tgcaggattg gttgtacgga
     1861 aagttttacc ccagttggca cagaaaccga tccaggtcca tatgcctcca gcaaaaatag
     1921 gctctgatcc aaatttgcgg tgcagtttta taaattcacg gtagaattct tcatcatcat
     1981 ggtaataatc ccaataaacc agttgagcac ctttaggtac tgtatcaaga agttcctgag
     2041 aaatttctat gtctatatca taatagcccc ccgatttgga agcagcacgg aaatacatgt
     2101 cactccagaa cattggcttc agattgtgct ttcttactat ttccagcacc cttgccagat
     2161 gctcgttcat tatatcaaat ttctttctta gaccgtgttt atcaaaataa gctcccattc
     2221 ccaatctcca tgcttcatcc ataccaatag ggatacgttt tgtacggaat ggtgcagctg
     2281 cagctacaat aagatcctca ataaacttat agactttctc ttcccctacc agaagagtct
     2341 cttcatcatc acgcatatcc caatatacag gccattttaa tatgtctata agatgagcca
     2401 gtgtttgaat gcagggaatc atctcaatac caaaaatatc tgcataatca tcacattctt
     2461 ttaaatcttc atatgagtac ttgcctctca tatatccaaa gtacggctgc tcctttacct
     2521 catagctgtc ctcacagtat agcataagca tattaagtcc cataacagcc atccttctga
     2581 ggaaattttt gactgtttcc acacctatta ctgcattacc ctgagaaacg tcaaacatag
     2641 caccattcat ggtaaattga ggctcttcag taatttcaaa atcctcgtca ttatgcttca
     2701 aggcctccag cagcaagccc aaagcacgga aaaaatgtat tttttctgaa taacgaatta
     2761 cagcgtccag acccttttta gctacataaa tatcacaccc ggatctcttt tccaccttaa
     2821 tatttaaaca atctttacca ttattactaa tctcgtaacc aaaatccccg gccagtattc
     2881 ctataccttt catacattcc tgcaaatctc ctgaaaaatg caacttcata attattttct
     2941 cctttcattt gtaccatttg cttaatatct ttgctgttta aattatttgt gccatttaca
     3001 tcattcgcgt tatttacacc atttatgtta tttgtaataa atttgcaagg atcagctgaa
     3061 gcattacttc tacctcagat tttacacgtg tcccgggcaa ttagcacaca ggcggaggta
     3121 aaaatatatc agagttaatt atttcagcat tcctgcagat agataagtac ctatcagctt
     3181 accttttaag gcaactttct aatctataat attttaacat acaatgctaa ttttttatag
     3241 tttgaaattt tactactata atgaattttc ttgacagcat acaaagtttg tatataataa
     3301 ggttgtcttt atatttaccg caattgccat tagtatatat tatttgtata aattgcttgt
     3361 acataatatt cacgtataac aaattaacat aaatcatcaa tttatgaggt gatgtctgta
     3421 tgggaaaagg aaaatatgag tatcttgaca taaaccagga aatacatgac gctgtcatgt
     3481 attttataaa aagccatcta ccatatatgg caaatcataa aacgaatctt tccccaggtg
     3541 atggcaacat ttcttagcaa gcttctttaa atccgtgtta gtatccaacc ctatccaact
     3601 gtctattgaa cctctgaaac agtatctttg ggcttcaaat tttctggttt tctcatcaat
     3661 tagaataaac ctcattagtg cctgatatcc tacgcattca ccttcaaata gatatattgt
     3721 aatcacattc ttctttacat acacttttgc atccagctct ttggagatac tattttgtac
     3781 aatgtttatc tcatttacag ttattatctg tggcaaattt ttccacaaat ataccattcc
     3841 atttggctct tcataaattt caaacccatt tggtattttc tcagcaagct tacctttacc
     3901 cattgtactt ttggaaaacc aatagatggg gttgcctttt ttgcttatac cttcgtgcag
     3961 ataatatctg tcacctttgt agttagtata atgaaccatt atataattca tcctttttta
     4021 tcatgatgtt aaaataaata actcactact taaaatgtac ttatgcatat gatattatac
     4081 tagaagccta cttgaaaacg cagtttgtac aacaagagtt acacttttaa tatgcaccct
     4141 gacataatga atttttttga caacatacat agtttgtata taataaagtt gtcttcatat
     4201 ttattgcaat taccattagt atataatatt tgtataaatt acttgcatat aatattcaca
     4261 tataacaatt aatgtaaacc atcaatttat gaggtgatgt ctatatggaa aaagaaaaat
     4321 atgattatct tgacataaac ccggaaaaac acggcgccgt tgagagttat gttatggaaa
     4381 taatcagtaa atatgaagat tatgaaggtc taagatatac ctacaaagat tatctgcaat
     4441 ggcctgaaga tgaaagatgg gaacttatag acggtgttcc ttatagtatg agccctgctc
     4501 catcaaaaag gcaccaggaa atttgcctgg cgttgtgttc ggaattttat aactacttaa
     4561 agggtgaacc ctgcaaagcc tacattgctc cttttgatgt tcgtttggtg gatgaaggtg
     4621 aatcagatga tgatgcttca accgttttac aacccgacat tgtagtaatc tgcgatagaa
     4681 caaaactaga taaggcaggt tgtaaaggaa ctcctgatct tgttgtagaa attacctcgc
     4741 catataccgc aaaaaaagat gttattagga aatttgccgc ttatgaaaaa tttggggtaa
     4801 aagaatactg gattgtgagg ccagaagagg aaacagtcac agtttttaaa ctcagtaatg
     4861 aaaacaaata tggacgtcca gaaatatact gcagaaacga taaaattcaa gtcggaattt
     4921 ttgatgatct aataatacaa ctggaagata tcttcaagga ttaaagcaga tcttcattgc
     4981 cagatgtaaa gttcattttt gtaatatact ttttaggtgt cggacttatc actaattttt
     5041 ctgctatatc ttgtatttta attttatttg aagaatcgca tataagcttc tgtgctttct
     5101 tcatcttttc agcaaaaagg aattcagaaa aattcatacc tgtttccttc ttaaataaca
     5161 tgctgaaata agtgtagttc atatttaata aattagcaac cacagccata ttaatgggtg
     5221 ggcgcatctt ctatatttga agaaaaatat ggcaagaaac gatttccatg tataatattc
     5281 acttcagcaa cggattgtag agtttaagtc tacagaaaaa ttctacccgt cacattcacc
     5341 atccatggtt ttggtgacgg ctcacgacat ccatgtcgtg aattacgaat ttttctttgc
     5401 cttaaacata acaatccgta agctttgttc aaattttata ctcggaaaat cgtatgctgc
     5461 catattttgt atcataagaa gaaaatgcac cccatattaa tatccgtctt gcctatattt
     5521 tcacgtatat attattttgc aatttatact gcatttaaat ttgcatttat attatatatt
     5581 caaataaaat atttgtcatt attaaaagtc attgatcata tattatgaat tagtgctatt
     5641 tttttgagcg aagtaagttt acttacatac acttatttat taaaatttct tagggaaaga
     5701 ggagatcgga tacaatgaaa atgctggtta cagggggctg cgggtttata ggctcccata
     5761 tagttgatat gctaattgaa agaggtcatg atgtgtgggt tattgataat ctatccagtg
     5821 gtaaattaga ttatctaaac actaaggcaa aattctataa aatggatatt acccagtcat
     5881 ctataaatga aatatttgag aaaatccact ttgatatgat tttccatgaa gcgtcaatgc
     5941 ataatgcttc aaattctgaa aaagatccaa ctctggatgc aaatattaat attatgggga
     6001 cattgaatat tctaaataat atgagaaagt tcaatataaa taaaattatt tatgcatctt
     6061 caacagccgt ctatggcaat gtacagcagc tgcctataaa ggaatcatac cagggaaagc
     6121 ctgcatcatg ttatggatta tcaaagtatt ttacagagta ttatattcgt ctgtattcac
     6181 atctttatga tatagactat tcaattttac gatattcaaa tgtgtacggt gaaaggcaaa
     6241 atgacagtga agaatgtaat gtcatatctt cattccttta tgactttaaa ctaggcaggc
     6301 aacctgtaat attcggagat ggtcaacaga caagagattt catttacata aaagatgtag
     6361 tttcagcaaa tttcgaagcc attacaaaag gtgcacgaaa catatataat atatgcacat
     6421 caacttctgc cacaataaat tacctttata atatactaag agatattttt atgacttcgc
     6481 atgaatcaca tgaaccatat gaactatata aaccatataa atcatatgat ttacatcaat
     6541 tacataaatt atatgaacct ttatataata aagggcaaaa ggaagatata aaagaaagct
     6601 cttttgataa cactaaagct atacaggacc tggattggat tccaaaatat tctttgcggg
     6661 aaggactgga gaaaatgctt ttcaatatgg acgggcaggt acaatctgat agacagacac
     6721 aatcaaggga acatatcttg tccaggagcg gatatctaca gactctatca aggaatgtcc
     6781 ctatttaaat aaacaatagt gtatttatta aatatgaaga aatatttacc agtagctatc
     6841 ataacggcgc ttagatttat tagggacata cctcaacgta aaacagacac tacctactct
     6901 aaggaggttt gtcccctaac atcagtttta gttatatttc aggaccagcc aaatcaaata
     6961 ctctatctgt aacatataaa gatacacctt taatagcctt ttccatatca atattaactt
     7021 tattaaattc aaggccgtcc ataaaccgct cataagccct ggaccttgcc tcaactaagg
     7081 cgttttcata tatataatca gaacataata aaacgtcacc atttattatt atttttttag
     7141 gattaaatat atttattata ttggctattc ctatacccag atactcacca cattctctta
     7201 aaacttcgcg tacatcctta tctcccattt caaatgcttc aagaaccata tcatatgtca
     7261 gaagctcatt cttattagta gtattactga tatgggtatt atttacatcg ctttctattt
     7321 ttgtgttatc gttcttggaa ttattaattg tttgggaaac acttttttct tccatactat
     7381 ttctattaag aattttttgc aactcagcac atttaccctc ttttaacagt tcgctgcact
     7441 gtgaaataat agtctctaca gagcacataa cctccagaca tcccctattg ccgcaaaaac
     7501 accggggacc atcttttttt actgtagtat gtcctatttc accaatcaca gcttcgttaa
     7561 tttcatttct atagaaatta ataattccaa ctcccattgc caggtctaga aatataatat
     7621 tttcatctga atgtatgcca tttttcctta acacagcaag tgccttggtt tttgagttat
     7681 tctgaatgta aattggaata tgaaaaatct tttcgaaata ttccttcagg gaaagacctt
     7741 tccaccttaa gcttgtggat atctccactc ttccatcatc aagtaccaga cctgaaacag
     7801 ctatacctat tccaataata ttagatgaaa gcaatgacct tttttctccc tgcagtaaat
     7861 aactgacacc cttttctatt atctttaaga cttcatcatt agtttcttgc ttatataacc
     7921 gaacctcatt tttacttatg atatttccat ccaggtcagt aagagccata gttatccttg
     7981 aaatttcaat atttatagat attatgctcg ctgctgaagg attaaatcta attaaagctg
     8041 cttttcttcc aacttctttg gaatctactc ttcctacttc aataacaagt tttttatcca
     8101 taagatatgt tactatattt gtaacagaag atgggctaag ccctgtactt ttagctattt
     8161 ccggccttgc agcttccgaa tgttgtctaa tataatttaa aactttcagc cgatttattt
     8221 tctgcatcag ctgtacgttc ccaaccgctc catttatttg caaaactact ctactcctta
     8281 cctacacttt aatttttcta tttgctccag atatactaaa ttattcttta ataatatagt
     8341 aaaatccata ggtggtcaat atagcatttg cttccgggcg cgtttatcaa atatggagga
     8401 tatggcagca tgcgattttc caaatatata atttgtacaa agtaacagca aattctagcc
     8461 tttgtttcta taatgagttg gggtcatacc tgtaaccttt ttaaatactc tagtaaaata
     8521 ataataatca ttaaaacctg ctttttccgc aatctcattt atagagaagc aggtagactt
     8581 aagaaggttg cacgcatgtc cgactctcaa gttcgttaaa tattccgtaa aagttattcc
     8641 cgtctgtttc ttaaaaagct gacttatata gcttggatta actgtaaaaa tctcagatat
     8701 atcgcttaaa gatgtatctt tgcagtaatt ctcgtttaca tattgaagaa taggtctaaa
     8761 agtctcgttt gttatatctt gagctgttag ctcctgtaca atacctgcat tttccaccag
     8821 ttgcctgcta agattttcaa gcatttcagt gaaatttttg tatgagtttg ttaattgctc
     8881 gtaatcgaaa atatattcgc cggatttaac ggagtcatgt gaataaaagg aatgtgtaac
     8941 tatattataa acttgaaatg catgtttaat attataagct ccttctgcaa ataccttttc
     9001 taccatacta aatgcatcct gtattaaggc tgcttcttct tttactatag aattttgaag
     9061 ctgcatcagt acaaagccaa gttcgtcctg ctccggctca gtatattgat atacatcact
     9121 atcccctgtc ataaaatatt gatacgcagc aactgatgct tcatctataa tatcatgtaa
     9181 atgatcggag gaataatata ccccgcttaa ccctgctcca attaagctct tctgtaaagt
     9241 gttgtttcgt aatgcttctg caaggtttcc tgaatttccc acacttttcg gatctctatc
     9301 gccatgcatg ccaggatacc ttgcaagata ccccttgatt gattcgggag aattgctgcc
     9361 acttagtata tatgcatttt tgttctttcc aattcttaga gaaatactat ttatctccgg
     9421 tatacatatt gtattgcatc caatagtaac aagagcttgt atgccagttt cttttatttt
     9481 ctcaacacct aaaatatcca ggattttggc ttttctatct cttccgaagc tgctattatc
     9541 atcaattata gtaagtagtt cagcttctat taaaattctg gattttttta ttacatcttt
     9601 agctttatta agcacaccaa ttatttcgtt ttcatcaaaa ggtttaagac aaaagcccaa
     9661 agcaccataa ttcaatgcct tctggacata tgcaaactcg gcatagccac taatcacaac
     9721 aaaacatgta ttgatatttt cttcacggac tttcttaata agctccaacc cgttaatacc
     9781 aggcattctt atatctgtaa atactatatc aggcttaagg ctggttattt cattgtatgc
     9841 ttctattcca ttatatgctt caccaacaat ttcaaatcca tattcagacc atttaatgct
     9901 tgcctttaaa cttttaacta ccatgctttc atcatctgca ataaatactt tatacaaaca
     9961 tatccctcct ggcaggtatt tttaacagaa ctcccgttcc ttctccttca cggctgtcta
    10021 ttgaaactcc ataattttct ccataagtaa gacgtaaacg attattaaca ttagtaattc
    10081 ctattcctat agttttcgta gttatatcat ccttattttc tctttcacct gtatctcctt
    10141 ccagtctttg tctgattaca caaagggttt cttcatccat tccggatcca tcatccataa
    10201 cccatatatt taaatcatcc gatttgttaa tctctgcttc tatataaaga ttcccctttt
    10261 caagtttcgt ctcaagtcca tgaaataccg cattttcaat aataggctgc aatatcattt
    10321 taggtacatt acaattcaaa acatcatcat catacttata aaagacatta aatctttcat
    10381 taaaccttac ctgttgaatc tgaatatagg atttagcaat ttccatctct tctctaagag
    10441 ttactatatc agctccctta acactatacc tgaatatttg gcccaggcat tttgctattt
    10501 caagtgtttt aaaagcattt tcatccaccg cgcagccctt cataacctca agagtattat
    10561 ataaaaagtg agggtttatc tgacttttta aatactccag ttcagccttt ttcttttcca
    10621 actctgcttc atatagttta gtactggttt ttacaagtct gtgagtaagg ttatctattt
    10681 catcaagaag agcattaaat tctccagcca taatttcaat ttcctcatac ccctccagac
    10741 ttactctttg tttaagttcc tttagatttc cctttcttat tgaaccaata tgattaacaa
    10801 attcgtttaa tgggttgagt atattactaa ttattagcat aaaaggtatc gacagtagta
    10861 taatggcaat aacaaataat actattgtca tttgctgaac ccatctaata tcacggaaaa
    10921 gttcacttac aggggcagca ctaattatcc tgccgtctat cctatcaata ttttcaccat
    10981 aaactacaaa actctcatta tttattatga tagttctatt atttatctgc ttatgtttat
    11041 tattttcgaa tgtatttgct atattatcaa atatagaatt attattatca ttacttgaat
    11101 ataaattgtc atttcgatct gtaagataaa accgggctga cgatttaata tcttctttat
    11161 tggtttcaaa tcccagcata tttttgttaa taatcaatgc agctataccg attttttccc
    11221 ccggactttt gtccatgctc acagaatata catttgtaat aacaataaag cagtttaaat
    11281 caaaatccgg caaattcgga tatcttatta aataagtacc ggtataataa atactttcct
    11341 ctacaggtat attttttatt atctcgttta atttctcatt atatcccctt agttggtact
    11401 tttctccgat attgcttata attactatat cataaatatc attttttatc tcctgcaagt
    11461 tagtacatat actatcgacc tgttgaaata atttataatg cctatatgga tctttttcca
    11521 tcataaattc ctgcaccgaa ctgttatagg caacattatt tagtaattta tcaagaatat
    11581 ctatgttagt agatatattc ttttttattt gaaaaaccat ttcagtcata tattcataac
    11641 tatctttttt gataaagtct gctatttgga aataagccac aaccagtatt gtaaatacaa
    11701 ctataccggc agaaacagca agtataatca attgcacttt aatctgcatt ttcttcaaaa
    11761 aattaagtat catttacacc ccctgtaata ttgcaaatga ttagtataaa catgttttaa
    11821 actattttat gttattttat attaatcact ttaagttctt tctattttat gtaatatatg
    11881 ctttaattgg aatatatttt ttaataatta tataacatat tgtatttaac actaataact
    11941 actattttgt ctatcattaa ttttattttt tatcaataat ttgcaaataa tgttattatg
    12001 tatactcaga atatgaccta tagatttatt ataacaatct tttattttta agacaagtta
    12061 ctttttttaa gaacataaaa agcacaggct tcttattttg ataaataagc cctgtgcttt
    12121 ttcttaaagt tcatttttcg caatactgca atactattta gtcttttgct taacttattg
    12181 ctttttatta tttaatacca agttctgctg cttttgcatt gaagtccttt attacttcat
    12241 catatccgtt tgcacggtgt tcttttatta tctggttcca tgtcttctca acatcatcgc
    12301 tcagtattgc ttttaccata tcatctgcaa actgttcaac atgtttatct tttgaagcat
    12361 aatctatgaa accggcgcga aggtctgtat taataggctt acagttttta atacgccaat
    12421 ccagttcttc attgcccatt tttagaattc cctcaggaac tgcaggattc ttatatgaga
    12481 aatcctgtgc ccatgatgaa agacttccaa ggcctgttat tggatacttc ttattaaggg
    12541 gatcataact accgctttca tttttttcac gggttataac tattttatct ccatctttct
    12601 tccaatctgt accttcaata ccaagtctta ttaagttaaa tccttcttcc gagaataaat
    12661 agtcaaataa tcttaaaatt ctgtcaactt taacatcgtc agcttttgcg ttaatataac
    12721 tctctgacca aggtgttggg gctactaatc tgtatttgtt tccatcagca gccgaccatg
    12781 gttttaaata agttacagct tcatcaaatt taaaatcagg gaaagtttta acgaacttgt
    12841 tgtttaatgc tgaaagagtt gtaggataac ctgtatgagc ataagctcct gcacggcctg
    12901 ttacaaattt atcctgacct tctgtgcctt ttattgtagc aaagtcttta tccaatccac
    12961 cagaatcata aagtctctta agctccttaa gaccttcaag gcagttttct gtcataaagc
    13021 cgggaagcca tccgccactt ttttcgtctc ttacccagta gttggcacca gtagcaacac
    13081 caggctcata ggaaagcata agggcagtaa gccagcttgc actgtaggct gttaatccta
    13141 cagtatcttt ctttccgttc ttatccgggt cattttcaac aaaagccttc atcagggtaa
    13201 taaattcatc catgttttcc ggctgttttg taatgccaac attctccatc caatcttttc
    13261 tcataagaac tgtaaggtca tttgcccaca tatcagcatt tggataggta ggacgtggta
    13321 tacagtagaa cttgccattt ggatctgcca aaggatatct gaatgcttgg atatcaggct
    13381 gatccattat ttccttaagg ctcgggtatg cactgaagtt gttaggcagt tcgtgaacaa
    13441 taccctctgt aatccatttt tcgtaattag gtgttgatat agcatcaatt gcgaacaaat
    13501 ctggtaactg agatgatgca gcccatatct gaatcttttg acgatagtca tcccatgtaa
    13561 ggttaacggg ttttaaagtg atattaagtt ttttgtagag agcatctctt actgcatctt
    13621 cttttacatc ttcaggtaat gcctgtcctg cgccccaata agccagtgat atttctacat
    13681 gctctttaag aggatcttca ggctgttttg tagtattatc acctgttgta gtcttattat
    13741 ctttgtttgc cttgtctttc ttgttacaac caataaagaa tgacataacc attactacag
    13801 caagaagaat tgctacaagt ttctttaagt tcttcataca tttgcctcct tgtaaatttt
    13861 gtttatatta acagctaaaa gctgctgtta actatattgt tccattatta ccgatgtata
    13921 tattactttt atttaatcaa ataaataaat tgtatttcct attactcttt aacagcccca
    13981 aacataattc cttttgtaaa atgtttttgg aggaatggat aaactagcaa aattggtaca
    14041 gtagcaacta caattactgc catttgcaga gattgtgaat aaactgtctt ctggctttcc
    14101 ataattgctg ctccaatact gcttgccata agctgagtaa agttcattat aatctctctt
    14161 aatactaatt gtagaggata aagctttcta cttgaaataa ataacaatgc tgtataatat
    14221 tcgttccatt tatctactga ataaaacaag gatactgttg ctattacagg tgcagacatt
    14281 ggaagtacta ttttatagag aatatagatg tcatttgcac catcaatttt agctgattcc
    14341 tcaatacttg ggggtaattc ctggaagaag ttttttaata gaatcagata aaacgtattt
    14401 atagcatgag gaattatcat aacccatatt ttgtttataa gccctacctt ttgaactgtt
    14461 aaataatagg ggataagccc tcctgagaaa aacattggaa ttaatatcat cgtaaaaaat
    14521 attttattgc caggcatatc ttttttggat aaagtgtatg cagcagcagt tgaaagacac
    14581 atacttacca atgtacccac aagagttaca aaaactgaaa caaaaaatgc actgtgcaca
    14641 attttttcct gtaaaatgag tttgtacgat tcaaaattaa ttgatgtagg tagaatatat
    14701 ataagctgct tttgcacttc tgcataatct gccaaagata taataattac ataataaaac
    14761 gggaatatag tgattattgc cattactgcc ataagtatat aattaaaaac atcaaatccc
    14821 gtagttcttt ttattaattt attttctaaa ttattactca aaataagcca acctcttttc
    14881 aatggatttt aaataaaatc gtttgctaaa atcatctatt gcatagtttt caactaggtt
    14941 acttctatta atatatacct cttccactca ttttcttcac tatagcatta cctgtaaaaa
    15001 gcattatgaa gttaacaact gacttaaaca aaccaattgc tgttgatgag ctaaaatctg
    15061 caccaataat gaaggtacgt ctgtaaatta atgtatcaat tatatcagcg acatcgtaaa
    15121 cagctgcatt atacatgttg aatatctggt caaatcctgc attcataata ttagcaattg
    15181 caagtatcag cattatacca attgtagacc tgattgccgg ccatgtaaca tacttcacca
    15241 gttgaaaacg ctttgctcca tcaactattg ccgcttcata tagttctatg ctgactccgg
    15301 atattgctgc aaggtatata attgtacccc accctgcttc tttccaatta ttagtaacat
    15361 acaataatgc tctaaaagtt gatttctgag ttaaaatgct aaccttctct cccccaaagg
    15421 caactatgat ctgatttagc actccctgat ccgagaggat gttaaaaaat ataccggtta
    15481 cgataatcca cgatacaaag tgagggaatg tgagaacagt ttgtacaaat cttttatatt
    15541 tactttttcc tatttcatta attagaagtg caagaattat tggaacggga aattcgaaaa
    15601 taattctttg tacgcttatt ataagagtgt tcgcaaaagc ttttttgaaa gcagcatcgc
    15661 tgaaaattct gtaaaagtgc tcaaatccat gtctggcagc ccatgggcta cgaagtattc
    15721 ccttggaata actaaaattt ttaaaagcaa gtacgacacc atacataggc atatatgaaa
    15781 atataatata gaatataact gcgggtaaaa taagcaaata gaaatactta taatacgaca
    15841 tccttttcca taaagagttt ttcgaattat ttagcttatt tattttttcc aggttgtttt
    15901 tttctctttg tactatacta tccatagaaa tttttagctc cttgtcattt tctacttata
    15961 attctatagc attttttttg tgcagtgtat acaaaatatt tatattttta taagaaataa
    16021 tacatttttt atatgtaagt aatatccaaa tataaagaaa gtacatattt atgtactttc
    16081 tttataaatt ttaatactta atgctacatc ggcatattag tattaactat atataattta
    16141 aacttagtgg atcaataaat ggtatgggaa ggcacctttc aaaaaattct gcaaagactt
    16201 catagttaga tgtggaatcc gagttggaga caaaatcctc tccggttacc ttagctccgc
    16261 caaaaatcag gtcatttaac ttttctttat cagatataat acatttttcc tctctaaatt
    16321 taaagcatgc gcctttttcg tcattttcaa attcaagttc attgataaac ttattgtcat
    16381 aaatttcata gaaatactgt cgtaaagatt ccataagact cttaaagtta actattttta
    16441 ttgtaccaat taaccttctt tcttctaaag gaatattatt ttctctgcag tagttaatag
    16501 ctgatgtatg atatgtcata agtcttccat ttatgctttt taaatcatat ttctccagca
    16561 ggattttaca tgctttaatt attaattctc tagaacctgc acaatcatat aaggttcctt
    16621 cgtttccttc aacaaccata taaaaatatg cttctaaacc ttctttactt tcaactacaa
    16681 ccactttttt ttgagctgtc ttttttctca tgaatttgcg ggaatccagc aattcataaa
    16741 atttatcata gtagcgaaca aatctaacat tttcctgctt ataaagattt gcaaggttat
    16801 aaagatcatc ctggctgcat tcttttatat tcagctctct aatattatat tcattgaaat
    16861 ctattttact tatgtcatta tctttaatct gatacatgta cattaaccct gcctgcgtac
    16921 ttccaatatt tctgtataac ctcctgtcac cagatattat aagtacatca accccatcat
    16981 tgtacattct actaacgcaa tcatcaagaa gagaggaagc ataaccatgt cccctgtagt
    17041 ctttcaatgt agcaacagct cctaatgacg ctgcatttat gctgcatccg tatattgaca
    17101 caggtcttat aacataattc atgttagata caggtttccc atcctcaaaa attactctta
    17161 cattatcgat gttctcctca caaaataaat ccgggaattc ttgaaacatt gtttgtccat
    17221 catcatcata tgtacgaaaa acctcattag aaagtctttc cactaaaggt agttcgctaa
    17281 cttttgcagc tctgggtcct tccattatac atccctccag tttttactta cattaatact
    17341 ttatataata acaaataagt tgtaaaaaat aaattacata ttgtatatat ttgtttattt
    17401 gtatattata attattagca taacaattat actgttatta taagtatcga gtgtatacag
    17461 ataataaaca aatagctata tttgcatcaa ttcagattat gattattaag tttaagggct
    17521 ttgatgcata tgctaaacaa ttattttgaa aagagggatt ttaatgctga aaattgatat
    17581 ccacgttcac acgaggatgc aaaaaggtgt tgaaagatta ggcggaggag attatgcatc
    17641 tcctgaagaa ttgcgagaaa tgtacaaaaa acttggtata ggcaaagctg ttattttacc
    17701 gggaataaac cctgagtgca tgaaccgtat tcaatctaat gaagaagcat atatattgac
    17761 ggttgagtat cccgaattat tctactggtt ctgcaatatt gatcctagaa tgggtaataa
    17821 ttctccaggc tccgatttat cccatcatat taattactat aaaaaactag gcgcaaaagg
    17881 gatcggagaa atatgctcaa acttgtattt tgatgatcca cttgtagaaa atctgttttt
    17941 ccacagtgaa aattgcaaca tgcctgtgct tttccatata ggacctcaaa taggcggctg
    18001 ttatggtctg gtagatgaag ttggcttacc cagactggag aaagagctga agaaatttcc
    18061 taacctccat tttattggcc attcacaacc attctgggcg gaaatcagca gtgatgtaaa
    18121 ccagaataac aggaacagtt atcctgatgg caaagttaca cccggcagag tagtggaatt
    18181 aatgagaaat tatcccaatc tttatggtga cttgtcagct ggcagcggtt ataatgcagt
    18241 cagccgtgat ccggaatttg gatatacttt catagaagaa ttccaggata agctttactt
    18301 tggaactgat atttgcgatc caaggaacga tatgaaactt tctttctggc ttgatgaagc
    18361 cctggaaaaa gataaaatat caaaaactgc ttatgaaaaa gtatgcttta agaatgcact
    18421 aaaattactg gaaaactaaa aatacataaa cataaaaata tatgtaaata caaagtaaca
    18481 gaaaggcaca gggacactcc tcactgattt cttgcgaagc atcatgagga agtgtccctg
    18541 aagtgtattt ctctaactta caccatatta tttcgaaacg atcttattta atgcatcttt
    18601 cacaattttt tctacagagt tgtcaagctg tgacgggtat aaataatata tagtgcttcc
    18661 atcaggttca tatccgcctt gttcaaacat ctcttctgtt ggtatataac ccgaaacacc
    18721 attgctgt
//
LOCUS       DAOSDD010000065        18641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1222_length_18641_cov_4.731572, whole genome shotgun sequence.
ACCESSION   DAOSDD010000065 DAOSDD010000000
VERSION     DAOSDD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18641
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1222_length_18641_cov_4.731572"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK733_07680"
     CDS             complement(<1..800)
                     /locus_tag="PK733_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ10456.1"
                     /translation="MQIFRAHVLVCGGTGCTSSNSQKVIDEFEVQLKKNSIDNEVKVI
                     KTGCFGLCAQGPIVVVYPEGAMYTMVKPEDVNEIVEEHLIKGRIVKRLLHAETHAEED
                     GQHKSMEHVDFFKKQLRVALRNCGVINPENIDEYIAHDGYRALAKALIEMKPQEVIDT
                     IKKSGLRGRGGGGFPTGLKWEFAAKSQDDKKFVCCNADEGDPGAFMDRSVLEGDPHSV
                     VEAMTIAGYAIGADQGYIYIRAEYPIAVKRLNIAISQAKEYGLLGKNI"
     gene            complement(822..1193)
                     /locus_tag="PK733_07685"
     CDS             complement(822..1193)
                     /locus_tag="PK733_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ10457.1"
                     /translation="MKSIAELEAIRKRTLEQMSIREKSERMRVVVGMATCGIAAGARP
                     VMNAFLEELKKRNINDVRVTMTGCIGACRLEPIVDVIDEKGEKVTYVRMTAEKAARVV
                     AEHIVNGRVCLDYTVGAEEKI"
     gene            complement(1177..1743)
                     /locus_tag="PK733_07690"
     CDS             complement(1177..1743)
                     /locus_tag="PK733_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ10458.1"
                     /translation="MRDLSLHLMDIVQNSIKAQASKIDIILTADAENDKFIIEIEDNG
                     IGMDESLLNQVKNPFATTRTTRKVGLGIPLLEASSARAGGKLEIKSEKGKGTCIKADF
                     QISHIDRLPLGNIGETMMGLVAANPETDFNLNLCNMKENFAFSVLEIKEQLDDVPIDN
                     SDVLIWIRDYISEGIKVIFGGVLNEIDS"
     gene            complement(1801..2307)
                     /gene="nuoE"
                     /locus_tag="PK733_07695"
     CDS             complement(1801..2307)
                     /gene="nuoE"
                     /locus_tag="PK733_07695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOJ10459.1"
                     /translation="MSGGKCCCGCVDEKEQKLQEIIQKYKDTKGGLIPVLHEAQDLYG
                     YLPLSVQKKISEGLNVSLSEIYGVVTFYTRFSLQPKGIYNIQVCLGTACYVKGANLIL
                     DKLQEKLGINVGECTEDGLFSLEACRCIGACGLAPVITINEDVYGRLKPEQIEDIIEK
                     YRKQKAAV"
     gene            complement(2753..3463)
                     /locus_tag="PK733_07700"
     CDS             complement(2753..3463)
                     /locus_tag="PK733_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ10460.1"
                     /translation="MKYYIDLHIHSVLSPCSDKDMTPNNIVNMAILKGLDIIAVTDHN
                     SAENLEAISDCACKSGILFVPGMEIETREEVHIVCLFPDVERALKMQEKVYKVLPQVK
                     NRPEIFGEQLIMDENDNIIGQLDKMLLTAADMSLEEVCHTMAELGGVMIPAHVDRDSY
                     SIISNLGMIPSEIKTGNLEISKNCDLGKFLSLYPQLSRYNFIRSSDAHNLGDILEQSS
                     VLELEEKSIECLLYTINY"
     gene            complement(3447..3779)
                     /locus_tag="PK733_07705"
     CDS             complement(3447..3779)
                     /locus_tag="PK733_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOJ10461.1"
                     /translation="MTVKEFAEKLGLAVLTGDSTLERDITGVYVCDLLSWVMSHAEKG
                     DAWITVQAHINIVAVAVLLEIPCIIIPEDINVEETTLKKASEENIAILRTGMDAFEIC
                     WRTHEILH"
     gene            complement(3904..5235)
                     /locus_tag="PK733_07710"
     CDS             complement(3904..5235)
                     /locus_tag="PK733_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOJ10462.1"
                     /translation="MENYFHSVTLDENSCKGCTNCIKRCPTEAIRVRKSKARIIKERC
                     IDCGECIRVCPYHAKKAITDSFDIIQNYKYKIALPAPTLYGQFNNKYSRNRILNGLKS
                     IGFDDVYEVARAAEIVTGATRKLIKEGGFKVPLISSACPAVVRLIQVRFPNLIDNIVK
                     IESPMEVAAKMAKNKAMKVHDLSANDVGVFFITPCAAKVTSVKAPYEKDMSAVDGVIS
                     MRDIYLKLLNTMDKSKSLENLEQSSAEGIRWASSGGEALGLGIDSYLAVDGIHNVIKI
                     FEEIENERLEDVNFVEALACTGGCLGGPLTVENTYLAKTRLKKQIEEADNISVQCNML
                     DEYDESLVWTGSIEHRPIMKLDEDIVVAIKKLETLEKINNNLPGLDCGACGAPSCRAL
                     AEDIVRGNANETDCIFKLRDKVRSLAVQMAELEAKMPPVMDKGKDNDKDIR"
     gene            complement(5279..5710)
                     /locus_tag="PK733_07715"
     CDS             complement(5279..5710)
                     /locus_tag="PK733_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ10463.1"
                     /translation="MSSNSIKLCYQIPGNDFTLAGEASSNVKKMLTQLGVSPDIIKKT
                     SIAMYEAEINAVIHANGGTAEVEIDSEKVSIKIIDEGPGIPDIDLAMQEGYTTAPDNV
                     REMGFGAGMGLPNIKRYADMLEIESKVGKGTTVRIEICLKT"
     gene            complement(5713..6060)
                     /locus_tag="PK733_07720"
     CDS             complement(5713..6060)
                     /locus_tag="PK733_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10464.1"
                     /translation="MRVDNIKEILDAEILVEGSNPDIDVCTACGSDLMSDVMAYGDEK
                     DILLTGLVNPQVIRTAEMMDIKMIVFVRGKKPDKNMMELAVDKSIILMTTRYPMFIAC
                     GKLSSAGIRGGSV"
     gene            complement(6313..7977)
                     /locus_tag="PK733_07725"
     CDS             complement(6313..7977)
                     /locus_tag="PK733_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOJ10465.1"
                     /translation="MNKNHMITVTFADGKIEKFNSGISLIELSKKYKENYPYTIIAAK
                     VNNEIRELTYMLNNDCKVEFIDLSRDDGMRIYIRSLTFILVKAVNDIFPERKVIINHS
                     ISKGLYYEIKGDKDLNADDVGKIKNRMVEIVNARIPFIKRTIPIEEAKNILKEKGRDD
                     RLRNIQQRLKPYITIYSCDEVDNYFYGYMAPDTGYIDKFDLKYYKNGVILLFPKKDQP
                     NILPEFKEQQKLFNVFRENRKWGSILGVESVGGLNDVIKSGNVRDLIRVSEALHEKKT
                     AQIADMIAESNNRKRVVLIAGPSSSGKTTFSKRLAIQLRVNGLKPVTIHLDDYFVDRE
                     RTPIDENGEMDFEALEAIDIELFNDHLIKMLNEEEVEIPIYNFPLGKREYSGRKVKLD
                     ENDIIIIEGIHGLNEKLTPSVPRENKFKIYISAITSMNIDDHNRVPTTDTRLLRRIVR
                     DNQFRGSSAVDTIKRWPSVRRGEEKNIFPFQEEADVMFNSAFRYEHGVMKIFAEPILA
                     GIDDSCPEYPEAKRLMEFLSYFLPIAEHDEIPNNSILKEFIGGSCF"
     gene            complement(8120..8563)
                     /locus_tag="PK733_07730"
     CDS             complement(8120..8563)
                     /locus_tag="PK733_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOJ10466.1"
                     /translation="MSLKEQLLNDLKTAMKEKDTVRKDSVQMVRSAVLQVEKDKKVTL
                     DDDGIIDIIAKEIKQRKDVLPDYEKSGRQDLVDRQLREIEILSQYMPQQLSEQELETI
                     VRETVRELGANSVRELGKVMQAVMPKVRGKADGKLVNQIAKKVLG"
     gene            complement(8582..8758)
                     /gene="rpsU"
                     /locus_tag="PK733_07735"
     CDS             complement(8582..8758)
                     /gene="rpsU"
                     /locus_tag="PK733_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOJ10467.1"
                     /translation="MSEIRVRENESLDSALKRFKRQCAKSGVLAEVRKREHYEKPSVK
                     KKKKSEAARKRKFR"
     gene            complement(8990..9334)
                     /locus_tag="PK733_07740"
     CDS             complement(8990..9334)
                     /locus_tag="PK733_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOJ10468.1"
                     /translation="MEDCIFCKIIRKEIPSSIVYEDDSVIAFKDINPVAPVHILMVPK
                     THLTSVKEINNENGSILVSIHLAANEIAKKMGIYENGYRLINNCGVNAGQTVPHLHYH
                     LIGGVNLGPKIV"
     gene            complement(9400..12039)
                     /gene="alaS"
                     /locus_tag="PK733_07745"
     CDS             complement(9400..12039)
                     /gene="alaS"
                     /locus_tag="PK733_07745"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOJ10469.1"
                     /translation="MQSYGLNELREKYLSFFESKDHLRLPSFSLVPRNDPSILLINAG
                     MTPLKPYFTGEQKPPHHRVTTCQKCIRTPDIERVGKTARHGTFFEMLGNFSFGDYFKK
                     EAITWAWEFITQELKMPEDKLWVTIYEEDDEAFEIWNKDIGLSSERIVRMGKEDNFWE
                     HGTGPCGPCSEIHFDRGADKGCGKPDCKIGCDCDRYIEFWNLVFTQFNKEADGTYAPL
                     KKKNIDTGMGLERLACVMQNVDNLFEVDTVRNILNYVCKLAGTGYGKDSKTDTSIRVI
                     TDHIRSTTMMISDGIMPSNEGRGYVLRRLLRRAARHGKLLGLDKPFLSDVSDIVINES
                     KTAYSELEEKRDYIKKVIKTEEERFETTINQGLAILNDYISQLEDEGKKVVPGTMVFK
                     LHDTYGFPLDLTREIAEENGLMIDEAGFKKEMEVQKNKAREALKSKEGSAWSQESRIG
                     LDKDFKTEFAGYGSTSCNASVSYILKDDDIVNTAMEGDKVVILLDKTPFYAESGGQVG
                     DTGYIRTETGMVRVEDCKKTHDGHYLHVGIVEEGILESNTAAEAVVDVKRRKAIARNH
                     TATHLLHKALRHNLGTHVAQAGSLVDDQKLRFDFTHFEPVSESDLMKIEEEINEKILE
                     DLPVKIEEMELDKAREKGAMALFGEKYGNIVRVVQIDGYSIELCGGTHMTSTAGIGFL
                     KITGETGVAAGTRRIEAVTGYSSLKYFRENEMLLNDIASTLKSSPQDAVKKIEALTSE
                     IKISHKEIDQLRSKLVGSKTDEILSKAVEINDISVITAKFEGMDMEALRNTGDVLKNK
                     LDDGVIILASEHGGKVNLIVTATKSAVEKGIHSGNIIREVAKVTGGGGGGRPDMAQAG
                     GKDPSKIDEAFKKALEIVKAGTK"
     gene            complement(12209..13258)
                     /locus_tag="PK733_07750"
     CDS             complement(12209..13258)
                     /locus_tag="PK733_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ10470.1"
                     /translation="MQKRKLMLLIIIIVISVLILIFIYSFRKKIGRILSPFFMAIVIA
                     YLVHPMVKQLEGRKISRPVSIIMVYSGVILVFTAVTIFIVPELVNSTKELINTVPDII
                     ANYQGMFNNILSNIRSSSWSSDIKDVLLNEINSSAEMIQNYTVNTLKRTMTRLIDSVA
                     IILDFMLALVIAYYFIKDSLFFRSQVISLIPKKWRKNVLQTGREINLVLSSFVQGQLL
                     TALIVSIMEIGGLLLLKVKYPLVLGSIGGIANIIPYFGPIIGAIPAVAVALVESPIKA
                     IWTVALFVVVQQIDNAFVSPKIIEGRVGLHPVTTIMAVLAGGEFLGITGMIISVPLVA
                     IVKAIFKRVVDEIVR"
     gene            complement(13426..13605)
                     /locus_tag="PK733_07755"
     CDS             complement(13426..13605)
                     /locus_tag="PK733_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOJ10471.1"
                     /translation="MGNGFTKGIIVGSLIGVSMVMAMNSGVMGSKSRRKMMKTGKRFL
                     RRSGNIVSDIAGVFR"
     gene            complement(13636..14298)
                     /locus_tag="PK733_07760"
     CDS             complement(13636..14298)
                     /locus_tag="PK733_07760"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOJ10472.1"
                     /translation="MKRITDILGLAVIFENGKKNPGIIKDVIFNIEEKQIKAFNVEKP
                     GINNSTRLILFQDVCEIGENAVIISDEAEAKATSRKLINYTGGQYTGGQSFTNYSERQ
                     SARQPGRPFAERQNDRKYSHIDKKIEEVNVYSKSGKDLGIVKDILFDCKTGYIEGFEL
                     TDGLIQDIIEGRKVVPLIGKYEYGKDYLLVGNDIEQEITTTGGGLRKKFLRIDSVYVT
                     DK"
     gene            14527..15915
                     /locus_tag="PK733_07765"
     CDS             14527..15915
                     /locus_tag="PK733_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOJ10473.1"
                     /translation="MENANYRRSLDLLESVGVKIPEIFLPEKDTDMRKWAVVACDQYT
                     SQPEYWDQVEKIAGDEPSALKLILPEVYLEKPGVEEKISRINKTMKEYLENGILTCQN
                     PGFIYLDRETSHVSSRKGLILTIDLEKYDYNKGSQTLVRATEKTVIERLPPRIKIRKD
                     APIELPHIQLLIDDPDMTVIEPLAAKTEMMEKVYDFDLMMGGGHVKGYKIEDTEIIND
                     IATALYKLSELDNFYKKYGVDSTKGVLLFAVGDGNHSLASAKAHWENVKAGLSPEAAA
                     SHPARYAMAEVINVHDKGLIFEPIHRVLFNVKPDDILHTMEGYFNKYSPTYIRTFCSK
                     EDMDSAVEEIKQTQQNAAKIHMLSFAAANCFGFSIIEKPAHNLETATLQEFLDLYLAE
                     NKDVKIDYIHGDDVVSSLGTKEGNMGFYLPPINKHELFKTVILDGTLPRKTFSMGEAE
                     EKRYYIECRKIK"
     gene            16029..17087
                     /locus_tag="PK733_07770"
     CDS             16029..17087
                     /locus_tag="PK733_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ10474.1"
                     /translation="MIEDIKLILEKAIRQKASDLHISPGIPPICRVNGRLIPVNDTVL
                     TSQDTEIFIKSVLSDKQLDTLYLKGEVDASISCCDSNRFRLNAFRQKGSYSISLRLVK
                     PEIPDFNSLGLPPVIIELSKLTKGLVLITGPTGTGKSTTLAAMIDWINTNRDAHILTL
                     EEPIEYVHTHKKSIVNQREIGTDSQNFSSALKAALRQDPDVILIGEMRDLESIGIALT
                     AAETGHLVLSTLHTIGSAKTIDRIIDVFPPYQQPQIRTQLSLVLQSVVSQQLIPTVDG
                     NDRVAAVEVMISTPAIRNLIRENKTHQIFNAIQTGSKSGMKTMDNSLIELYRANRISF
                     DEMMNYAVDHDYIKKMVT"
     gene            complement(17352..17792)
                     /locus_tag="PK733_07775"
     CDS             complement(17352..17792)
                     /locus_tag="PK733_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10475.1"
                     /translation="MEKSSGKNTVIHFNSESGYALVLSIILVVVIFILGSAILFSIAS
                     ETKTNEVMEQREIASYLAYSGIEHGLAILEATAYNDNPVLPSASFEVFNEGNKIGKYQ
                     ITQLTKTNLEAVGTYEIGGSPSIIRTTVIQAIIDSNGKVIVKKN"
     gene            complement(17801..>18641)
                     /locus_tag="PK733_07780"
     CDS             complement(17801..>18641)
                     /locus_tag="PK733_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10476.1"
                     /translation="DLIWGGGTITGNVYVGGKLIIQGWPTVNCSLVNVAGDVELLGGT
                     IGVDDYKANRFNVYTGGNLIIKGWSRINGNVDTIIVNKDLEVNGGSIKGKSSTIRTNV
                     YVNNSVKLQQCNLSNTNLKYVVNYSSGSTYNSSVSITKIKGNQIPEIPGFTIYEGFEM
                     PIYELPPLKPDEWYNINGYKSELTLSNNKKIFVNNFNPASVDASSNVIIVSKKDVNLN
                     VKSWGKLTGVVFAPNGKVTFTGGGFTGIVIARDGFFVNSGGIKVESRNISEFIKNVED
                     YPF"
BASE COUNT         5401 a         4004 c         2766 g         6470 t
ORIGIN      
        1 aaaatgtttt tacctaaaag tccatattct ttggcctgag atatagctat attcaatctt
       61 tttaccgcta tgggatactc agctcttata tatatataac cctgatccgc gcctattgca
      121 tatcctgcta tagtcattgc ttcaactact gaatgagggt ctccttccag gacgcttctg
      181 tccataaaag cacccgggtc tccttcatcg gcgttacaac atacaaactt tttgtcgtcc
      241 tgactttttg ctgcaaattc ccacttaagg ccggttggga agcctccgcc ccctcttcct
      301 ctcaagcctg attttttaat agtatcaatt acctcttggg gtttcatttc tatcagtgct
      361 ttggctaatg ctctatagcc gtcatgtgca atatattcat caatattttc cggattaatg
      421 actccgcaat ttctcaatgc cactcttaat tgttttttga agaaatcaac atgctccatg
      481 gatttatgtt gcccatcctc ttcagcatga gtttcagcat gtaaaagtct ttttacaatt
      541 ctccccttga tcagatgttc ctcaacaatc tcatttacat cttccggctt gaccattgta
      601 tacattgcac cctctggata aactacaaca atggggcctt gagcacacaa accaaaacat
      661 cctgttttta taaccttaac ttcattatca atactgttct tctttagttg tacttcaaat
      721 tcatcaataa ctttttgaga atttgatgat gtacaacctg tacctccaca aacaagaaca
      781 tgcgctctaa atatctgcat cagtaaccct cctcacaaca actaaatttt ctcttcagcc
      841 ccaacagtat agtcgagaca aactctgcca ttaacaatat gctctgcaac cacgcgtgca
      901 gccttttcag ctgtcatcct aacataagta actttctcac ccttttcgtc aataacatca
      961 actatgggtt caagtctgca agcacctata caaccggtca tagtaactct aacatcattt
     1021 atatttctct ttttaagttc ttctagaaaa gcgttcataa ccggccttgc accagctgct
     1081 ataccgcatg ttgccatacc tacaactact ctcattcttt cgctcttctc tcttatgctc
     1141 atctgctcga gagtcctttt tctaatcgct tccagctcag ctatcgattt catttaatac
     1201 acctccaaaa atcaccttta ttccttcact tatatagtca cgaatccata tcagtacatc
     1261 gctgttatcg atagggacat catccaattg ctcctttatt tccaaaacac tgaatgcaaa
     1321 attttctttc atattgcata aattcaggtt aaaatctgtt tccggatttg ctgctacaag
     1381 acccatcatt gtttctccga tgttacctaa tggcaatcta tctatatggg atatttgaaa
     1441 atcagccttt atacaagttc ctttaccctt ttccgatttt atttcaagct ttcctcctgc
     1501 tcttgcagaa gaagcttcca aaagcggaat acccaatcct accttccggg tagttctggt
     1561 tgttgcaaaa ggatttttta cttgatttaa caatgattca tccataccta taccattatc
     1621 ttctatttca attataaact tatcattttc tgcgtctgct gttagtatga tgtcaatttt
     1681 tgaggcctgt gctttaattg aattctgtac aatatccatt aagtgtagag ataagtccct
     1741 cattgttaaa atcctcactg tcgatattca atatcctttt tgatattcaa catttccttc
     1801 ttatacggct gctttctgct ttctgtattt ttctataatg tcttcaattt gttcaggctt
     1861 aagtcttccg taaacgtcct cgttaatagt gattacaggt gccagaccac aagctcctat
     1921 acatctgcaa gcttccaggg aaaataagcc atcctcagta cactcaccaa catttattcc
     1981 cagcttttcc tggagtttgt caagtataag atttgcaccc tttacatagc aagctgtacc
     2041 aagacaaacc tgaatattgt atataccctt tggctggagc gaaaaacggg tgtaaaaggt
     2101 aactaccccg taaatttcac taagagatac gttaaggcct tcagatattt ttttctggac
     2161 acttaacggc aaatagccat acaaatcctg ggcttcatgt aacacaggaa tcaggccacc
     2221 ttttgtgtct ttatactttt gaattatttc ctgcagcttc tgctcttttt catcaacaca
     2281 gccgcagcag catttacccc cactcatctg cactacctcc taattctaat caataatcat
     2341 aatgcttaat ttataatttt taatccttaa ctgttaatcc ataattgaaa aataacacca
     2401 aggattaatc attaactata gataacgtac aaaagaaact acattataaa agttttgtgc
     2461 gctaatgtta cattaatgaa ttcgtaaaaa ttcaaattaa tccatgtaga atcatttttg
     2521 cgttatatat actaaatttc ggactcatta aatacggtat ttatttgaca cattaaaaag
     2581 cagtgtttct tgcactgcta tataatatgt taagtattta acaatttccg ccccaattat
     2641 atcaaattta atttaattat tcaataagtt ttacgaaaat attggcataa tatttgaatt
     2701 aatttaatga tttgagttcc aggaaccaaa tggttactgg attttaatgt ttttaatagt
     2761 ttattgtata caagaggcac tcaatgcttt tctcctcaag ctccagaacg ctactctgtt
     2821 ctagtatatc tccaagatta tgagcgtctg aagaccgtat gaaattatac cttgataatt
     2881 gcggatataa tgacaagaat ttccctaaat cacaattttt tgatatttca agattacctg
     2941 ttttaatttc cgatggtatc atcccgaggt tggatattat gctgtaagaa tccctgtcta
     3001 cgtgagcagg tatcatgacg cctccaagtt cagccatagt atgacagact tcttctaaac
     3061 tcatatctgc agctgtgaga agcattttat caagttgtcc gataatattg tcgttttcat
     3121 ccatgattaa ttgctctccg aaaatttccg gtctgttctt aacttgtggt aaaaccttat
     3181 atactttttc ctgcatctta agtgcccgtt caacatcagg aaaaaggcat actatatgaa
     3241 cctcttcccg tgtttcaatt tccattccag gtacaaacaa aatgcctgat ttacaggcac
     3301 aatctgaaat agcttcaaga ttttcggcag aattgtggtc agttaccgct attatgtcca
     3361 gtccttttaa aattgccatg ttcactatgt tattaggagt catgtcttta tctgagcaag
     3421 gtgaaaggac tgaatgaata tgcagatcaa tgtaatattt catgagtcct ccagcatatt
     3481 tcaaaagcat ccatgccggt tcttaaaatt gctatatttt cttccgatgc ttttttcaaa
     3541 gtcgtctctt caacattaat atcttcagga attattatac acggaatttc caacaataca
     3601 gctacagcca ctatatttat atgagcttgg acggttatcc aggcgtctcc tttttcagca
     3661 tgagacataa cccagcttag taagtcacat acatatactc ccgtgatatc ccgttccaga
     3721 gtagagtcac ctgtaagaac agccaaacca agtttttctg cgaattcttt tacagtcaag
     3781 atgcacaccc cttatctcta tgtcttatac ccatatatta tacttatgcc ttatttctat
     3841 cctcatcttt atctttatct ttatctttat ctttgtcttt atctttgtct ttatcttata
     3901 tctttatctt atatctttat cgttatcctt acccttatcc attaccggcg gcattttggc
     3961 ttccagttca gccatttgta ctgccagact tctgaccttg tctctgagct tgaaaataca
     4021 atctgtttca ttggcattcc cgcggacaat gtcttccgca agagctctgc agcttggtgc
     4081 tccgcatgct ccacaatcaa gtcccggaag gttattgttt attttttcca gcgtttccag
     4141 cttcttaatt gcaacaacta tatcttcatc cagtttcata attggcctgt gttcaatact
     4201 acctgtccag accagacttt catcatattc atcaagcatg ttgcattgaa cagaaatatt
     4261 gtccgcttct tctatttgtt ttttcaaccg ggtttttgca agataggtat tttcaactgt
     4321 tagtggtccc ccgaggcatc ctcccgtaca agcaagagcc tcaacaaaat taacgtcctc
     4381 aagtctttca ttttcaattt cctcaaagat ttttattaca ttgtgtatgc catcaacggc
     4441 aagatagcta tctatgccca gtccaagagc ctctccgccg gaacttgccc atcttatgcc
     4501 ttcagcgctt gattgttcaa gattttcaag actcttactt ttatccatag tatttaaaag
     4561 ctttaaatat atgtctctca ttgaaataac cccatctact gcagacatgt ctttttcata
     4621 aggtgccttt acgctggtta ctttagcggc acaaggcgta ataaaaaaaa cgcctacgtc
     4681 atttgcggat aaatcatgca ctttcatggc tttattttta gccattttag cagctacctc
     4741 catgggagac tcaattttga ctatattatc aattaaatta ggaaaacgca cctgtattaa
     4801 cctgactact gccggacaag cagatgaaat aagaggtact ttaaaaccgc cttctttaat
     4861 aagcttcctg gttgcaccgg ttacaatctc tgccgccctt gctacttcat atacgtcatc
     4921 gaatccaatt gattttaatc catttaatat cctgtttctt gaatatttat tattaaactg
     4981 accataaaga gttggagcgg gcaatgctat tttatattta tagttctgaa ttatatcaaa
     5041 ggaatcggta attgctttct tggcatgata gggacatacc cgtatacatt caccgcaatc
     5101 aatgcagcgt tcttttatta tgcgagcttt acttttacgc acccttatgg cttccgtagg
     5161 gcatcttttt atgcagtttg tacaaccctt gcaactattc tcatccagtg taacagaatg
     5221 aaaataattc tccatattct caaccccttc caaaacagga aaaggaccta aagttttatc
     5281 aggttttaag gcatatttca attcttacag tagtgccttt acccaccttg gattctattt
     5341 caagcatatc agcatatctt tttatatttg gaaggcccat acctgcacca aagcccatct
     5401 ctcttacatt atcaggtgct gttgtataac cctcctgcat cgcaaggtcg atatccggta
     5461 tacccggtcc ttcatcaatt atttttatac ttactttttc gctgtcaatc tctacctctg
     5521 ccgtccctcc atttgcatgt attacagcat ttatctcagc ctcatacata gctatggaag
     5581 tctttttaat tatatcagga ctaactccca attgagtcag cattttttta acattacttg
     5641 aggcttcccc cgcaagtgta aagtcattcc cgggaatttg atagcatagt ttgattgaat
     5701 tgctgctcaa ccctatacac tccctcccct tatgccagca cttgataatt tgccacaggc
     5761 tataaacata ggataccggg tagtcattaa tatgatgctt ttatctaccg ccagttccat
     5821 catgttttta tcaggcttct ttccccttac aaaaactatc atcttaatat ccatcatttc
     5881 agcagtcctg ataacctggg ggttaaccaa tccagtaagc aaaatatcct tttcatcacc
     5941 ataggccatt acatcgctca taagatcaga tccgcaagct gtgcatacat ctatatcagg
     6001 gttgcttcct tcaaccagta tttccgcatc aagtatctct tttatattat ctactctcat
     6061 aacaccaacc tcttttaaat tcagggattt atagataacg cacaaaagaa actgcattac
     6121 aaaagttttg tgcgcttatg ttaccttaat taatacgtaa aaaatccaat ttgttccttg
     6181 taaaatcatt tttgcgttat atttagtcca tatacatctc atcattcaat aggatcaatt
     6241 gcttaaatca attgctgagt taattatagc atattgttaa ctgattaaca atcctttaat
     6301 ctcattgact cattaaaaac aacttccccc tataaactcc ttcaatatgg aattatttgg
     6361 tatttcgtca tgttcggcaa tgggcagaaa atagcttaag aactccatga gtctttttgc
     6421 ttcaggatat tcggggcatg agtcatctat acctgccaat ataggttctg caaaaatttt
     6481 cattactcca tgctcatacc taaaggctga attaaacatt acatcagcct cttcctggaa
     6541 aggaaatatg tttttctctt ccccgcgtct tactgaaggc cagcgcttta ttgtatctac
     6601 agcactgctt cctctgaatt ggttatctct tacaattcgt ctgagaagcc gtgtgtctgt
     6661 ggtaggaact ctgttatggt catcaatatt catggatgtg attgcgctta tataaatttt
     6721 aaacttattt tctctgggta cggacggcgt taacttttca ttaagaccat gaataccttc
     6781 gatgataatt atatcgtttt cgtccaattt cacttttcta cctgaatatt cccttttccc
     6841 caaaggaaag ttgtatatgg gtatttcaac ttcttcttca tttagcattt tgattaaatg
     6901 atcgttgaat aattctatat caatagcttc taaagcttca aaatccattt caccgttttc
     6961 gtctatcgga gtcctttctc tatctacaaa ataatcatcc agatgtattg taacaggctt
     7021 taagccattc actcttaatt gtatggccag cctttttgaa aatgttgttt tccctgagga
     7081 tgatggacct gcaatcaata ctactctttt tctattattg ctttcagcta tcatatcggc
     7141 tatctgggct gtttttttct catgaagggc ttcagatact cttataaggt cccgtacatt
     7201 tcctgatttt attacatcgt ttagtcctcc tacactttct acccccagga tactgcccca
     7261 tttcctattt tcacggaata cattgaaaag tttttgctgt tctttaaatt caggcagtat
     7321 atttggctga tccttcttgg gaaatagtag aatgaccccg tttttataat atttaagatc
     7381 aaatttatca atatatcctg tgtctggagc catatatcca taaaaatagt tgtcaacttc
     7441 atcacagctg taaatagtaa tatagggttt cagacgctgc tgaatatttc ggagccgatc
     7501 gtcccttcct ttttccttca atatattttt agcttcttca ataggtatag tacgctttat
     7561 aaaaggtatt cgtgcattaa caatttcaac catcctgttt tttattttcc ccacatcatc
     7621 tgcgttaaga tccttatctc cctttatttc ataataaagt cccttactga ttgaatggtt
     7681 gattataact tttctttcag ggaatatatc gttaacagct ttaacaagaa taaaagtaag
     7741 acttcgtata tatattctca tcccgtcgtc tctagaaaga tcaataaact ccaccttaca
     7801 atcattattg agcatataag tcaattctct aatttcatta tttactttag ctgcaattat
     7861 tgtgtatgga tagttttctt tatacttttt actcaattcg attaatgaaa taccgctatt
     7921 aaacttttca attttgccat ccgcaaatgt tacagtgatc atatggtttt tattcacacc
     7981 aaacacctcg catattttat tttatatgga cacaaaaaca ggcgggataa aaatagggct
     8041 tattaagccc tattaatacc ttgcatatga tttgtttttg ttatcatata ttatattcca
     8101 aacatctaat attatcaaat cagcctaaca ctttctttgc tatttgattt acaagtttac
     8161 cgtctgcttt tcctctgacc tttggcataa cagcctgcat tactttacca agttcccgga
     8221 ccgaattggc cccaagctcc cggactgttt ctctgacaat ggtttcaagc tcttgttccg
     8281 atagttgctg tggcatatat tgtgataata tctctatttc tcttagttgt ctatctacta
     8341 aatcctgtct tccgcttttc tcgtaatcag gaagaacatc tttcctttgt tttatttcct
     8401 tggctattat atctataata ccatcatcgt caagggtgac cttcttgtct ttttcaacct
     8461 gaagcactgc agaccttacc atttggacag agtccttacg gacagtatcc ttctctttca
     8521 tcgctgtttt cagatcattt aataattgtt ccttaaggga catagcacat atgcctcctt
     8581 atcacctaaa tttccttttt cttgcagctt cagatttctt ctttttcttc acacttggtt
     8641 tctcataatg ctcccttttc cttacttcag caaggacacc tgacttagcg cattgtctct
     8701 tgaatctttt aagagcacta tcgagagact cattttccct taccctaatt tcagacacgt
     8761 acttccctcc ctccgatggt atcaattgtg ccagaactaa gctccatatt ttgctgtgtt
     8821 tttttccaaa tcatttgtag cacatattat attatatctt attatagaaa tcaaagtcaa
     8881 tattagctat agtaatttta cactaacctg cggtaatttt atgctaatat tgcactattt
     8941 cctataagtt caccaatgtt cttttccaca cgggaaataa aacttgtgat taaacaattt
     9001 taggtcccag gtttaccccg cctatgagat gataatgaag gtgaggtaca gtttgccccg
     9061 cattaactcc gcagttgttt atcaatctgt agccattctc atagattccc atcttttttg
     9121 ctatttcatt ggcagcaagg tgaatactta caagtatact tccgttttca ttgttaattt
     9181 ccttaacact tgtaagatga gttttgggta ccataagtat atgcaccggt gcaactggat
     9241 taatgtcctt aaaagcaatc acgctatcat cttcatacac tattgaagat ggtatctctt
     9301 ttcttataat tttgcaaaaa atgcaatctt ccatgtgatt tcacctccta tcgaaacatt
     9361 ggtaccgaaa catcgggacg gttctttcgt ttcattttat catttagtac cagccttgac
     9421 aatctccagc gcctttttaa atgcttcatc tatttttgat gggtccttgc cacctgcctg
     9481 agccatatcc ggtcggcctc caccgccgcc gcctgtaact tttgcaactt ctcttataat
     9541 attccccgag tggatgcctt tttcaacagc actttttgta gcagtaacaa ttaaatttac
     9601 ctttcctcca tgctctgatg caagtattat aactccatca tcaagtttgt tctttaacac
     9661 atccccggta tttctcaggg cttccatatc cattccttca aattttgcag taataacact
     9721 tatatcgttt atttcaactg ctttcgataa tatctcatcg gttttacttc ctacgagctt
     9781 gcttcttagt tgatcaatct ccttatggga aattttaatt tccgatgtta atgcctctat
     9841 ctttttaacc gcatcctgag ggcttgattt taatgtagaa gcaatatcgt taagaagcat
     9901 ttcattttca cggaaatact ttaggctgga atacccggtt acagcttcta ttctccttgt
     9961 gcctgcagca actccggtct cccctgttat tttcaagaaa cctatacctg ctgttgatgt
    10021 catatgtgtg ccgccacaaa gctcgatgct atagccatcg atttgtacaa cccttactat
    10081 attgccatac ttttcaccga ataaagccat agcacctttt tcccgagctt tatccagctc
    10141 catttcttct atcttaacag gaaggtcttc aaggattttc tcatttatct cttcttcaat
    10201 cttcattaaa tcactttctg aaacaggttc aaagtgggta aaatcaaatc tgagtttttg
    10261 gtcgtccaca agtgagcctg cctgtgcaac atgagtaccc aggttatgac gaagtgcttt
    10321 atgcagcaaa tgagtggcag tatgattcct ggctatggcc tttctccttt ttacatcaac
    10381 tacagcctca gcagcagtat tactttccag tatgccttcc tcaactattc caacgtgcaa
    10441 ataatgtccg tcatgagtct ttttacaatc ttcaactctt accattccag tttcggtcct
    10501 gatatatccc gtgtctccaa cctgtccgcc actttctgca taaaaaggcg ttttgtcaag
    10561 gagtataact accttatctc cttccattgc agtattaact atatcgtcat cttttaaaat
    10621 ataggatacc gatgcattac agcttgtact tccatatccg gcaaattcag ttttaaaatc
    10681 tttatccaaa ccaatccggc tttcctgtga ccaggctgaa ccttctttac ttttcagtgc
    10741 ttcacgtgct ttgttctttt gaacttccat ctctttttta aaacctgctt catcaatcat
    10801 aagaccgttt tcttctgcaa tttctcttgt aagatcaagc gggaatccat aggtatcatg
    10861 aagtttaaac accattgtcc ctggaacaac tttcttgcct tcatcctcta attgactgat
    10921 gtaatcattt aaaattgcaa gtccctggtt aatagtagtt tcaaaccttt cctcctctgt
    10981 tttgatgact tttttgatat agtcgcgttt ttcttcaagt tcagagtatg ctgttttcga
    11041 ctcattgata actatatcgg agacatcaga aaggaaaggt ttatcaagcc caagaagttt
    11101 cccgtgcctt gcggctctcc tcaagagtct tcttagaaca tatcctcttc cttcatttga
    11161 aggcattatc ccatcagata tcatcattgt agtgcttctt atatggtctg taattacacg
    11221 tatggaggta tcggttttgg aatccttacc ataccctgtg ccagcaagtt tgcaaacata
    11281 attcaatatg ttgcggactg tatccacctc aaaaaggttg tctacattct gcataacaca
    11341 agccagtctt tcaagaccca taccggtatc tatattcttc ttttttagag gagcatatgt
    11401 cccatctgct tctttattga actgggtaaa taccaggttc cagaactcaa tatatctgtc
    11461 acagtcgcag ccgattttgc aatcaggttt tccgcatccc ttatctgctc ctctatcaaa
    11521 gtgaatctct gaacatggcc cgcaaggtcc tgtaccatgc tcccagaaat tatcttcttt
    11581 acccattctt acaattcttt cagaacttag ccctatatcc ttgttccata tttcaaaagc
    11641 ttcatcgtct tcttcgtaaa ttgttaccca aagcttatct tccggcattt ttaactcttg
    11701 agtaataaat tcccatgccc aggttatcgc ttctttttta aaataatccc caaaggaaaa
    11761 gttacccagc atctcaaaga aagtaccatg tcttgctgtt tttccaactc tttctatatc
    11821 aggcgtcctt atacatttct ggcaggttgt taccctatgg tgcggaggtt tctgctctcc
    11881 tgtaaaatat ggctttaatg gcgtcatacc tgcatttatc aagagaatac tggggtcatt
    11941 ccgaggtaca agggaaaaac tgggcagcct taaatgatct ttgctttcaa aaaagctcaa
    12001 atacttctct ctaagttcgt ttaatccata gctctgcatt attaaaacct ctcaattctt
    12061 tctacatata ataaatagtt aataatattt aataacacct ggtaatattt agtaaatctt
    12121 tataattata atattttaca ttcattataa ataataatat attaaagtat gtaaatagtg
    12181 tcaagaagga aaacaatatg ggggcatttt atctcactat ctcatctact acccttttga
    12241 atattgcctt aacaattgca actaacggca ctgaaataat catacctgtt atacccagga
    12301 attctcctcc ggcaagtaca gccattattg tggtaacagg atggaggcct acccttccct
    12361 caattatttt aggggaaaca aatgcattat ctatctgctg tactaccaca aataacgcta
    12421 ctgtccagat tgctttaatt ggtgattcca ccagagcaac tgccactgca gggatagctc
    12481 caattatggg tccaaagtat ggtatgatat tagctattcc acctattgag ccaagtacaa
    12541 gaggatattt gactttcaga agcaacagac ctccaatttc cataatgctt acaataagtg
    12601 ccgttaaaag ctgcccctga acaaaactag ataacaccag gtttatttcc ctgcccgtct
    12661 gcaaaacgtt ttttctccac tttttaggta tcagagaaat tacctgggat ctaaaaaaga
    12721 gtgagtcttt gatgaaataa tatgctataa caagtgccag cataaaatca agtataattg
    12781 ctaccgagtc tatcagcctc gtcattgtcc ttttaagggt attgacagta taattttgaa
    12841 tcatttcagc acttgaattg atttcattaa gcaatacatc tttaatatct gaagaccagc
    12901 tgcttgatct gatatttgat aatatattgt taaacattcc ctgataatta gctataatat
    12961 ccgggactgt gtttattagt tcttttgtac tatttactaa ttcaggtact ataaatattg
    13021 tgactgcagt aaagacaagt attacacctg aatacaccat aataatgcta accggacgtg
    13081 aaatttttct accttcaagc tgcttaacca tagggtgaac cagatatgct attacaatag
    13141 ccataaaaaa cgggctcaaa attctcccta ttttttttct gaatgaatag ataaatataa
    13201 gtataagtac ggaaataaca atgataatta ttaacagcat caattttctt ttttgcatat
    13261 ttatacttcc tctatagatt caggaaatgg gggaatacat attcccccat ttcctgccgg
    13321 aacaatttga ttcatactta ctgattcatg gtaattatta acggtattgg tacaccatat
    13381 taattaatga tattgattcg tgatatcttt tcatatattg attttctatc tgaatacacc
    13441 tgcaatatca cttacaatat ttcccgacct ccttaaaaat ctttttcctg ttttcatcat
    13501 ctttctcctg cttttacttc ccattacgcc gctattcata gccatgacca tggaaactcc
    13561 gattaagctt cctacaatta ttccttttgt aaatccgttt cccatgcaaa cacctccctt
    13621 caacagcaac tgaatttact tatcagttac ataaacagaa tctatcctta agaatttttt
    13681 tcttaaccca ccaccggtgg ttgtaatttc ctgttcaata tcgttaccta ccagcaaata
    13741 atcttttcca tactcatatt taccaattag cggtacaact tttcttccct cgataatatc
    13801 ttggataagt ccgtctgtta attcaaaacc ttcaatgtat cctgtcttac agtcgaataa
    13861 tatgtccttt actattccca aatccttacc tgactttgaa tatacattta cttcctctat
    13921 ttttttatca atatgggaat actttctgtc gttctgtctc tctgcaaagg gacgtccagg
    13981 ttgtctagca gactgtctct cactataatt tgtgaaggac tgtcccccag tgtactgtcc
    14041 cccagtgtaa tttatcaact tccgggacgt tgctttagct tcagcttcat cactaattat
    14101 tactgcattt tcacctatct cgcatacatc ctgaaaaagt atgagccttg tgctgttatt
    14161 tatacccggc ttttcaacat taaatgcttt aatctgcttc tcttctatat taaatatcac
    14221 atcttttata atacctggat tttttttgcc attttcaaaa attacagcta atcccaggat
    14281 gtcagtaatt cttttcaatc gatgctcctt tagcataatt ttcacaatta attttatata
    14341 attttttgtt tttatagttt attatagcaa gtatattctg aaatctatta tcccttattt
    14401 tgaataaaat aattcacata cttaattatt tgactacaat acaaaattaa gataaaattt
    14461 aactgctgag taaatataaa tgtaagtttt taaaactaat aaaaaaggtg aggttatcga
    14521 ggttatatgg aaaatgctaa ctatagaaga tcattagatt tattggagtc tgttggtgta
    14581 aagattcctg aaattttttt gcctgaaaaa gatactgata tgagaaaatg ggccgtcgtt
    14641 gcctgtgacc agtatacttc ccaacctgaa tattgggacc aggttgagaa aattgcaggt
    14701 gatgaaccat ctgcattaaa actcatttta cctgaagttt atcttgaaaa accaggtgta
    14761 gaagaaaaaa taagtagaat caacaaaaca atgaaggaat atttggaaaa tggtatttta
    14821 acctgtcaga acccaggatt tatatattta gaccgggaaa caagccatgt gtcttcaagg
    14881 aagggattaa ttctcactat agaccttgaa aaatatgatt ataacaaggg ttctcagaca
    14941 ctggtgaggg ctactgagaa aacagtaata gaacgacttc cgccaaggat aaaaattaga
    15001 aaagatgctc caattgaatt gccccatata cagcttctca tagatgaccc tgatatgacg
    15061 gttattgagc ctctggcagc caaaacagaa atgatggaaa aagtctatga tttcgattta
    15121 atgatgggtg gaggtcatgt aaaaggatac aaaattgaag atactgaaat catcaatgat
    15181 attgcaacag cactttacaa gctttctgag cttgataatt tttataaaaa gtatggagtt
    15241 gattcaacaa aaggagtgct tctatttgct gtaggtgacg gcaatcattc cctggcttct
    15301 gcaaaagccc attgggaaaa tgtgaaagct ggcctttctc cggaagcagc agcatcacac
    15361 cctgcaaggt atgccatggc cgaagttatc aacgtacatg acaaaggcct gatcttcgaa
    15421 cccatacaca gggttttatt taatgtaaaa cctgatgata ttttacatac aatggaaggt
    15481 tattttaaca agtattcccc aacctacatc aggacattct gttcaaaaga ggatatggac
    15541 agtgctgttg aggaaatcaa gcaaacacag caaaatgcag caaaaattca tatgctttct
    15601 tttgctgctg ctaattgttt tggtttttca attattgaaa aaccggctca caaccttgaa
    15661 acagctactc tacaagaatt ccttgatctg tatctggctg aaaataaaga cgttaaaata
    15721 gactatattc atggggatga tgttgtttcc tccctcggta caaaagaagg taatatggga
    15781 ttttacctgc cgccaattaa taagcatgag ctctttaaaa ctgtaatact tgacggtaca
    15841 ctccccagaa aaacattttc tatgggagaa gctgaagaaa aaagatatta tattgagtgt
    15901 agaaaaatta aatgattgca gataattttt ctagatttaa tttttccaga ttgatttgta
    15961 tagatttaat ttgtttaaat ttatgaatta tttgtagata tttgtaaatt ctgaaaggac
    16021 gagcatttat gattgaagat ataaaattaa tacttgaaaa agctataaga caaaaagcct
    16081 ctgatcttca tatttctccc ggtataccac ctatttgccg ggtaaacggc cggcttatcc
    16141 ccgttaatga taccgtgcta acaagccagg atacagaaat attcataaaa tcagtccttt
    16201 cagataagca actagatacg ctctacctaa aaggtgaagt agatgcttca atttcttgct
    16261 gtgacagtaa tcgctttagg ctaaatgcat tccggcaaaa gggttcatat agtatttccc
    16321 taaggcttgt aaagccggaa ataccagatt ttaattctct tggtctgcct ccggtaatca
    16381 ttgagctttc gaagcttacg aagggattgg ttttaatcac cgggcctaca ggtacgggaa
    16441 aatctactac tttagctgct atgattgact ggataaacac taatagggat gctcatatat
    16501 taacgttgga agaacctata gaatacgtgc atactcataa aaaaagcata gtaaaccaaa
    16561 gagaaatagg aactgattcc cagaattttt ccagtgctct taaagctgct ttaaggcagg
    16621 atcctgatgt tatccttata ggtgaaatga gggacttgga aagtattggt attgcactga
    16681 cagctgcaga aaccggtcat cttgttttaa gtaccttgca cacgatagga tcagcgaaaa
    16741 caatagaccg gattatagat gtatttcctc cctaccagca gcctcaaata aggacccagt
    16801 tgtcactggt attgcaaagt gttgtatcac agcaactcat ccctactgta gatggtaatg
    16861 atagagtagc agctgttgag gttatgattt ctacacctgc aataaggaat ttaataaggg
    16921 aaaataaaac tcaccaaata ttcaatgcta tccaaacagg ttcgaaatcc ggaatgaaaa
    16981 ctatggataa ctcattaatt gaactatatc gggctaatcg tatcagtttt gatgagatga
    17041 tgaactatgc agttgaccat gattatatca aaaagatggt gacctgataa attatcatat
    17101 ttccatatta actacacata tatctgcagc aaatacgatt acccattttt taagattaga
    17161 aaggcttcga aattcatatg aactatgata tttcatgacc tgttctattg cttcgttttt
    17221 tttttataac atcagtgcaa tccgccaatc ggattgcttc acaccgcttc aataaataca
    17281 taaagataaa actactatag ttttatttcc ttgatagtat ttattataac tcatttactt
    17341 aattattaca attagttctt tttaactata accttgccat tactatcaat tattgcctgt
    17401 attaccgttg ttctaataat acttgggctg ccgcctattt catatgtacc aactgcctcc
    17461 aagttagttt tagttaattg tgttatctgg tattttccaa ttttattacc ttcattaaat
    17521 acttcaaagg atgctgaagg taaaacagga ttatcattat aagcagtagc ttcaagaata
    17581 gccaaaccat gttctattcc ggaataagca agataactcg caatttccct ttgctccatc
    17641 acttcgttag tttttgtctc agaggcaata gaaaagagta tagcagagcc taaaatgaat
    17701 attacaacaa caagtataat tgataatact aaggcatacc cactttcact attaaaatgt
    17761 attacagtat tttttccaga tgacttctcc atttaacacc tcaaaaagga taatcttcaa
    17821 cattttttat aaactcacta atattccttg attctacctt tattcctcca ctatttacaa
    17881 aaaaaccatc ccttgctata actattcctg taaaacctcc tccggtaaaa gtaactttac
    17941 catttggagc aaacacaact ccagtaagct taccccaact ttttacattc aaattaacat
    18001 ccttcttact tacaataatt acattgcttg aagcatctac gctagcaggg ttaaaattat
    18061 taacaaaaat ttttttatta tttgataaag ttaattctga cttgtatcca ttgatattat
    18121 accactcatc tggttttaga ggaggtaatt catatattgg catttcaaaa ccttcatata
    18181 tagtgaaccc aggtatctcc ggaatttgat tgccttttat tttagtaatt gaaacactgg
    18241 aattataggt agaaccactt gaataattaa cgacatattt taaattcgta ttactaagat
    18301 tacattgttg cagtttaaca gaattattaa cataaacgtt cgttctaata gtgctgcttt
    18361 tcccttttat tgaaccgcca ttaacttcca aatccttgtt tacaataatt gtatccacat
    18421 tgccatttat cctactccaa cctttgataa tcaaatttcc gcctgtatat acgttaaatc
    18481 tattagcctt ataatcgtca actcctatag tcccaccaag cagttcaaca tctccggcaa
    18541 catttactaa gctgcaattt actgtaggcc agccctgaat aattaatttg cctcctacat
    18601 aaacatttcc ggttattgta cctcctcccc atatcaaatc a
//
LOCUS       DAOSDD010000066        18380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1239_length_18380_cov_5.029359, whole genome shotgun sequence.
ACCESSION   DAOSDD010000066 DAOSDD010000000
VERSION     DAOSDD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18380
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1239_length_18380_cov_5.029359"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1500)
                     /locus_tag="PK733_07785"
     CDS             complement(94..1500)
                     /locus_tag="PK733_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10477.1"
                     /translation="MLGPWRTHKEYQSYLEENFFQFFKADPKNVMQYKDALSKLYILD
                     LDSLKPLLSSYYSITGAPAKNQPELIRSFILMSELKEHSITNWVLKLQSNEILCNMIG
                     LSRDEIHQVGSYYDLINRVWLSNPEIDYEFEHSLHTFKRKPAKKLEKNKKQPPRHPGI
                     IQKFVDLALEGKIFESRPEMLMQQIFAKIGVEPAAKEGLHGDTEKLCVSGDGTCVNSG
                     ASNFGNKVCKCVENGIYNCDCPHKFSDPYARWGWDSYHGQWFYGYTEYILSVYNKELK
                     YDLPLYLRMVQAQRFNGVSAIVALAEARKLYPEFTFDSFCGDSAHDNYATYELLHKLD
                     IKAFIPLNEKNKDNFTYPPHIKVDEHGVPYCMGNPKMINWGYTPDRCRIKYRCPLVLG
                     RIESCDCKNQCSPSEYGRCIYIKPSWDLRLFTVVPRNSDEWREQMKSRITSERVNRRI
                     LNDYGLEYSHTRGKKRTF"
     gene            1985..2914
                     /locus_tag="PK733_07790"
     CDS             1985..2914
                     /locus_tag="PK733_07790"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOJ10478.1"
                     /translation="MNRTILELVRKVSHANETFSIIYSILSDLKAKKNRRLDDETYFK
                     KRYRSYVGKDLILDNPQTFNEKLLWLQIHDHNPLYTKLADKYLVKKNVADTIGEKYVT
                     PLIGVWNSADEIPFEKLPNEYVLKCNHDCGSVFIKRSGDTVNEKKIRKKFNKALKRNY
                     YDVGRVWSYKNIKPLVFCEELISTKDGKVPKDYKIFCFDGEPKFAFVASNRGEDTKFD
                     FYDMNWNRIPVKQHYPNSKLDIPKPEQFNEMVECARKLSKNIPQVRVDFYIDANGNVI
                     FGEMTFCHFSGKEPFEPEEYDSLFGKYIKLPKI"
     gene            3247..4386
                     /locus_tag="PK733_07795"
     CDS             3247..4386
                     /locus_tag="PK733_07795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ10479.1"
                     /translation="MKEKIKIIIAADLVPTQSNIEYFKTKNMDALIGHELVTLLKSAD
                     IRIFNLEAPITERRDKIIKSGPHLKIDPVAINGIIELNPSVLSLANNHIKDYGENGIR
                     DTIDYLRNNDIPYVGVGENIDKAREGVTITIKGHIISVISFAEHEFSIARDNQCGANP
                     YDPLTSFDDVKRIAERSDYVIVLFHGGKEHYRYPTVEQKRICNKFLSVGANIVVCQHS
                     HCIGAAENINGGVAVYGQGNFIFNLCDDERWKSSLLIEIELCELSSISYIPIVKCGAT
                     IRIANEEEAIHIIDGFESRCKEILEEGFLEKNQKIAAKRAANYYLKKLHGAGFFFRVL
                     DKILSGRIIRYIYRHSSIRAELLNCIECETHREFITDYLRNSLID"
     gene            4404..5525
                     /locus_tag="PK733_07800"
     CDS             4404..5525
                     /locus_tag="PK733_07800"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ10480.1"
                     /translation="MNKKVVIIGDEHYNTLGVIRSLGEAGVRPDIIIIDSCKAKSWVL
                     TSCYVKHGTIVERNNEKIIAVLLDKSNGQNERIYLVPTSDFAVKLIDDNFNVLREHYV
                     LPSIGNKAGELTRLMNKRVMAQYAEDAGFLIPKTKSHFVLPDFKNDKELFESINVLYP
                     VIVKQENAFAAKDKIWIVKNKIELKSALSKCINEKVIIQQFIEKDEEIGIQGVGFGSS
                     AEPVVPGVVHKIRTSLNAIGSTTYADLRSIAQNDPVQQVKNFIKNIGYYGIFDIEVLR
                     KGESYYFIECNFRNGAYGYSYTRMGANLPMIWMGENVGVNTFNYTSLKLINTFSDYKH
                     IKNGNISWYKWMWQLATANVYLTANCKDPKPFFYRLIMR"
     gene            5534..6271
                     /locus_tag="PK733_07805"
     CDS             5534..6271
                     /locus_tag="PK733_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOJ10481.1"
                     /translation="MYLIRLDDACPYYDVEKWQHVEGILDMYKIKPIVAVIPKCQDKM
                     FVNQYQYNDLFWDKARHWQDKGWTIALHGYNHVYTNMNAKGLNPINNFSEFVGLSYEE
                     QCEKIYNGVKIMNDHGLHPKCFVAPAHSFDTNTLIALKNVSCIRVISDTVANDIYYKH
                     EMLFIPQQCGSFIRMPLKIITCCYHPNAMQAKDIHALENVLAKYKDKFTNFDSLKFKK
                     RNFGFFDYMLKNIYFARRKMKRINIHY"
     gene            6288..7298
                     /locus_tag="PK733_07810"
     CDS             6288..7298
                     /locus_tag="PK733_07810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10482.1"
                     /translation="MKIKQVKLKALEDTWKKMYSSNNLLFPYSSYEFASCYNKYFHLG
                     SKRLFMKQLFFLISNDDKDVAIIPLICRKDDYYLYGDMCATGYLDIITAGEITDDEYS
                     KIFELIFSKIGNATLNLNKINDRSRLFHYLLKYYTEEYSSPCVNISFSNDYDTYMKSL
                     SKSVRQNIRTAYNRIQRENRTCNLKVIINESLPSEIINEGMKIYNKREENRNKKKSSL
                     VTKIIRRHFNPITTSTLFIPNNIHALFFIDGDIAAFLSGYITNDNKTIVVPRHAININ
                     YSSYSAGTLLITELIRYCIDNTEIRNLDLSRGDEKYKYSLGGTTHYNHSFVLKHQREG
                     KK"
     gene            7299..8450
                     /locus_tag="PK733_07815"
     CDS             7299..8450
                     /locus_tag="PK733_07815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ10483.1"
                     /translation="MKKVIYLTTSLFPEGQAYSVRVIGFAALFKSLGYSVHVIADSTR
                     QTDIVRNKIYTFDYGTYEISGKNGRLSSNLTNKYFAIAALKKQLSNSDACAVVANASS
                     DRFNDVLLLCHKNGVKLILEVCEWYDVISSRLGLIDYRNYRMNRMMEKGYSKADSIIA
                     ISRYLEQHFSKRNNIVIRIPTIFDTQQLLYTIDSNNQKIVLVHAGSSVIHKEKFKHIM
                     MAIQKIGKDTPIEYRIYGSSRDIVLKNIDGDDALLHALGEQVKIMGTIPQAEVNKAYQ
                     QADFSIFMRDRKCSTSAGFPTKLAESMAAGTPVIANDTGDIGLYLKDGYNGFLINDNS
                     IEEIESTLRKVISLSSKQKKNMRENARKTAEASFDYRSYIDIVKELFKD"
     gene            8496..9020
                     /locus_tag="PK733_07820"
     CDS             8496..9020
                     /locus_tag="PK733_07820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ10484.1"
                     /translation="MKFIKLIKIIIDKYQQASNPVKYAKKIGVNIKGRVHLYGKQIWS
                     TEPWIITLGDNVYLTNGTRFITHDGGTLILRDKVPDLEITKPITIGNNVYVGVNTTFL
                     PGVNIGNNVIIGACSVVTKDIRDNSVAAGNPARVIKTIDEYFEKVQAESLHLGHLKGK
                     EKDIALRRYYKYDK"
     gene            9010..10314
                     /locus_tag="PK733_07825"
     CDS             9010..10314
                     /locus_tag="PK733_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ10485.1"
                     /translation="MINKVLSIVKKSVMKLFDKDKLKVYVYTASNIATMLMAFAISVL
                     LTRWLNPEIYGKYKYAVNILFTLPTYFEFGIHFCSSRIVAEDNADESHGVVPVSIGVL
                     SALAVIITFGLYMFIFTCKSFEIYTGIEKLNDVRIIFPFIIVFASNIVVTQLYQGTGK
                     IYRLSAYSFIQYAIVIVGVVVGHYATLQLTFEYCIFVYIISNAVVQMPVLMRIRIQRD
                     LIIPNFKRLFHDIKSFGIKIYLSSFATSGASTLIGLICGSIYGYSEYGYYTLAISLAQ
                     FFTFISSSMATVKFKDNVKGKYLKKSDMLFMLFMNSLIYLVFSIMINKVFFWFYSPEY
                     AKSIAYLRILAISYIFNGMALFYNRFFIARKMGNIIMRNSFMVAAVNVIASIILIPLL
                     KINGLVIASLIASAYNFFQYIITYRSYLKNLKNGKKAVLDNE"
     gene            10307..11620
                     /locus_tag="PK733_07830"
     CDS             10307..11620
                     /locus_tag="PK733_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10486.1"
                     /translation="MNNFNTLTYANKNNIIVSSKISKQRDNTVLQLILFYVFIIVAII
                     LTDKGISLARVIAFWGILFIAVLQKPISIFMFMVATLPLSHIARMENSSLTLLPFLTI
                     ILLIKIIITNKAISLNKAMPLFIIGIISIINEIIRWSSMITSLVFIMTWLIPVFAYCI
                     KQEINHKIVLISFAYVISSFISIIGSTLFPNAARMITSYTQYSYRAVGFSQAWSYGIN
                     LVIASAFILIIYKQTNKRLVLSAIAIAILLYNAILSGTFSILIGIALVLFCFSIQTLK
                     KEKVLLLLLIPLLFVLGYSIMKAFFQIRGDISDNGRIEIWMFYMKHLLSNFSVFMFGI
                     GGGNIASMASKYQMSTAHNGILELLIEVGFCGTVLLILYSKNLLRNIKLSFRKNILSL
                     PFLIYLSFLLTQGGASSYSIVIFISLLISSSEQTKNQGVEQYVCK"
     gene            11607..12599
                     /locus_tag="PK733_07835"
     CDS             11607..12599
                     /locus_tag="PK733_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOJ10487.1"
                     /translation="MFVNKTLLITGGTGSFGNAVLKRFLNTDIGQIRIFSRDEKKQDD
                     MRRLYKNDKIKYYIGDVRDIASVKNAMHGVDYIFHAAALKQVPSCEFFPLEAVKTNVL
                     GTDNVLTAAIECGVKKVICLSTDKAAYPINAMGISKAMMEKVVIAKSKTVDPDKTLIC
                     CTRYGNVMASRGSVIPLFVDQIKSGQALTVTNPSMTRFLMSLEEAVELVVFAFQNAQS
                     GDIMVQKAPASTIGDLAQAIKELFNADNEIKIIGTRHGEKLYETLLTKEEYIAARDLG
                     NYYRVPADKRDLNYDKYFIEGSQQLSSNGEYTSHNTKRLNVEQIKEKLLTLDYI"
     gene            12627..13736
                     /locus_tag="PK733_07840"
     CDS             12627..13736
                     /locus_tag="PK733_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HOJ10488.1"
                     /translation="MKILVTGAKGFIGRNLVAELKNRKHTNIFEYDIDTDLSLLDAYC
                     REASFVFHLAGVNRPKDQSEFMKGNFGFTSVLLDTMKKHNNTCPVMISSSIQAALDNP
                     YGKSKKAGEELMFAYGKETGARILVYRFPNVFGKWCRPNYNSAIATFCNNIAHDLPIK
                     VNDRNVVMKLVYIDDVVQELINALNGTENRIGDFCEVPVVHTITLGEIVDLICSFKNS
                     REERSIPDMSDEFTKKLYSTYLSYLPTKQFSYPLKMNVDNRGSFTEFIKTPDRGQVSV
                     NISKPGIIKGNHWHHTKNEKFLVVSGKGVIRFRKVDTDEIIEYYVSGDKLEVVDIPTG
                     YTHNIENLGDTDMVTIMWANECFNPDKPDTIFLEV"
     gene            13772..14878
                     /gene="wecB"
                     /locus_tag="PK733_07845"
     CDS             13772..14878
                     /gene="wecB"
                     /locus_tag="PK733_07845"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ10489.1"
                     /translation="MTILGTRPEIIRLSEVIKKCDKYFEHILVHTGQNWDYTLNQIFF
                     EDLGLRKPDYYLDAVGVDLGETIGNIIAKSYKVLSEVKPDALLVLGDTNSALSAISAK
                     RLKIPIFHMEAGNRCFDENLPEETNRRIVDHISDVNLCYSEHARRYLNFEGVVKERTY
                     VTGSPMAEVLTANIEKIRNSKILEELGLEKGKYILLSAHREENIDIEENFFALMNAVN
                     AMAENYNMPVIYSTHPRSAKFIEQRRFKFHPNVRSLKPFGFSDYNNLQINAFCVVSDS
                     GTIPEEASYFKFPAVSVRTSTERPESMDKGNFIIGSITTEQVLQAVDLAVAMYENGDL
                     GVTTSDYADENVSTKVVKIIQSYTGIVNRMVWRK"
     gene            15210..16577
                     /locus_tag="PK733_07850"
     CDS             15210..16577
                     /locus_tag="PK733_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOJ10490.1"
                     /translation="MLLPIGGHLIKGRDIGIKNNKIYKMKYYSHFDEKVHWKNVKHIV
                     ENPEKVARHGFFPFIHYTHNIEKFNKKVGRKPPKLREIFYSSHIDRYIYELYAYKLNN
                     IYNKRVIKDCTNKCAVAYRDNLHKSNIHFAKKVFDAIRRYNQAYVIIGDFTGFFDNLD
                     HSYLKDRLCSLLETTILPDDYYAVFRNITKYSYFELEKLKEITGLKYKELNKQNRVLE
                     LKDFRQYKRECLRKNKNNYGIPQGSAISAVLSNIYMIDFDKKINDFVTSKKGIYMRYS
                     DDFVIVIPKCEIVEFQKMWVFIDNIKRSVKNLELQLEKTQIFEYNNLHVKSCNSLLFH
                     EVPDGKNIINYLGFSFDGKVITIRDKTISKYYYRMYRKIDAIAKKGGYTKKGKKIPMN
                     TLYKRYSFYGEEQTKDNRGNFLTYVNRAKEVFGPNEAIDRSTKKHWGKLQKRLHKFWK
                     EHREN"
     gene            16957..18336
                     /locus_tag="PK733_07855"
     CDS             16957..18336
                     /locus_tag="PK733_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ10491.1"
                     /translation="MQKIFRDPIYRLISFDKDKDSLLLKLINTYEFQRLRRIRQLGVS
                     WLTYPTAVHTRFSHSIGVAYLAGRIFDKLSLEDEIKFDDDDDNYVLSKSELKLLLQTT
                     ALLHDIGHGPFSHAFEDVISQKHEEWSRKIIQDENTNINKIILYDEEISNYISPKNRK
                     KFPQWISNILNGIFPLYYINEIISSQLDADRLDYLLRDAYMCGVDYAKFDLEWILNNV
                     NVGIITHEEGRKGIVINAEKGIYSLESFIISRYHMYEQVYFHKTTRAAEKLIHNIFIR
                     LKELIGEGKTEKIGQIDNSILAVFTGNINIENYLKLDDFMLISYINKWASESKDDLLK
                     YLCSNFINRRLYKLVTEGENVQIISSEESDKIYEFFKDKKLVKKYYFVEDSYTEVAYK
                     DDYLFGKKSSEDAAHIWLIDRFGNLVDLGEASAIINALRNNIKKKYRIYCDRSVVQEF
                     FAKKFLGGM"
BASE COUNT         6543 a         2493 c         3487 g         5857 t
ORIGIN      
        1 cagaagtttt tcggcaagca aatccgcaaa gctaaaacca agttttttga gtctggcatc
       61 cagaagaata ttcaacgaat ggatcaaact ccactagaag gtgcgtttct tcccacgtgt
      121 atgagaatat tccagcccat aatcattaag aatacgcctg ttaactcgct ccgaagtgat
      181 tctgcttttc atttgttctc tccattcatc agaattacga ggaacaacag taaacaagcg
      241 aagatcccag gagggcttaa tgtaaatgca ccttccatac tcagaaggag aacattgatt
      301 tttgcaatca caggattcaa tcctacccaa gaccaaaggg cagcgatatt taattctaca
      361 gcggtcaggg gtgtaacccc agttaatcat tttagggttt cccatacagt aagggacacc
      421 gtgttcatcc accttgatat gagggggata tgtaaaatta tctttattct tctcgttcaa
      481 aggaataaag gcctttatgt ccaatttatg caggagttca tatgtggcgt aattatcatg
      541 agcactatcg ccacaaaaac tatcaaaagt gaattcagga tagagcttcc ttgcttcggc
      601 gagagcaaca atggcagaaa caccattaaa tctctgggct tgaaccatcc tgagatacag
      661 agggaggtca tacttaagtt ctttattata tacggaaaga atatattcag tgtaaccata
      721 aaaccactgg ccatggtaac tatcccaacc ccagcgtgcg taagggtctg aaaacttgtg
      781 tgggcaatca caattgtaaa tgccattttc aacacattta cagactttat tgccaaaatt
      841 gctggcaccg gaattaacac aggtgccatc tccggaaaca cacaattttt ccgtatcacc
      901 atgaagacct tctttagcgg caggttcaac tcctattttg gcaaagattt gttgcataag
      961 catttcgggt cttgattcga agatcttgcc ttctaatgct aagtcaacaa atttctgtat
     1021 aatacctgga tgccggggtg gctgtttttt attcttttcc agctttttag cgggtttgcg
     1081 tttaaatgtg tgaagagaat gttcaaattc atagtcaatc tcgggatttg acagccatac
     1141 ccggttgata aggtcatagt atgaaccgac ttgatggatt tcgtcccttg ataaaccaat
     1201 catattgcag aggatttcat tagattggag ttttaaaacc cagttggtaa tgctgtgttc
     1261 ttttaattct gacattagaa tgaatgaacg gataagctcc ggttggtttt tggcaggagc
     1321 gccggtaatt gagtagtatg aggagagtaa gggttttaag gaatcaagat caaggatata
     1381 cagttttgat aatgcatctt tatactgcat tacgttttta ggatcagcct taaaaaactg
     1441 aaaaaaattt tcttcgagat agctttgata ttctttgtgt gtacgccaag gtcctaacat
     1501 atgaaacacc tctaaaatca agtagtatgg ccaaaaaaga accatccaaa tcgattattg
     1561 gggcgttttg aaaatgtcaa gggtttttgg aaaaaaagtt taatttttta gcgacttttt
     1621 gggggtgaac taaaaaaggc aactcttgaa agtcgctaat ggcaatatgt ttaagttttc
     1681 gaaattacta ctattgtaat aggaggtaat catcataata cacttggagt aattagaagt
     1741 ttaggtgaaa aaggtgtatg tgttcacgta gtaattatat cttcataatg aataaatttc
     1801 atatgtcaaa attagcttcc cagagtggct ttatcacacc caaaacacgg attattaata
     1861 cagaagaatt tatttgagaa gaaaaacttc caaatgatat agtatatcct tgtataataa
     1921 aaccaacagt aagtgctata ggaagtatag aagatgatgc taaaaagttt taggaggaaa
     1981 gaacatgaat cgaactattt tagaacttgt tagaaaggtt tctcatgcta atgagacttt
     2041 ttctattatt tatagtatat taagcgattt aaaagcaaag aaaaacagga gattggatga
     2101 tgagacttat tttaagaaaa gatatagatc atatgtagga aaggatttaa ttttagataa
     2161 tccacaaact tttaacgaaa aattgttatg gctacagatt cacgatcata atccgttata
     2221 cacaaagtta gcagataaat atcttgtgaa aaaaaatgtt gctgatacaa taggtgagaa
     2281 atatgtaaca cctttaattg gtgtatggaa ttctgcagat gaaatacctt ttgagaaatt
     2341 gcctaatgag tatgtactta aatgtaatca tgattgtggc tcagtattta ttaaaagatc
     2401 tggagatact gttaatgaga aaaaaattag aaaaaaattt aataaagctt tgaagagaaa
     2461 ttattatgat gtaggaagag tatggagtta taaaaatata aaaccgcttg tattttgtga
     2521 agaattaatt tctacgaaag atggaaaggt tccaaaagat tataaaatat tctgttttga
     2581 cggagaacct aaatttgctt ttgtagcatc taatagaggt gaagatacaa aatttgattt
     2641 ttatgacatg aattggaatc gtataccggt taaacaacat tatcctaata gtaaacttga
     2701 tattcctaag ccagaacagt ttaacgaaat ggtagagtgt gctagaaaac tatctaaaaa
     2761 tatacctcag gttagagttg atttttatat tgatgcaaat ggaaatgtaa tatttggaga
     2821 aatgacattc tgtcatttct caggtaagga accttttgaa ccagaggaat atgattcact
     2881 atttggcaaa tatattaaat tacctaaaat ttagcaaatt agataatagg ttattggaca
     2941 tatataatac ttcttaatga agtgtacctg tatatgaatt ataaaaaatg agtagagatt
     3001 tacttaataa atattcaata tttaataaaa ctactagtgg cagtaatttc tgattataag
     3061 gatgaatatt taacgaatat ttattctgag tataatcagc tatggaacag agctagctta
     3121 gggtaaactt tggcaggctt gtaatattat atggttatat tcttaaaatc cttgataaaa
     3181 gtgctttgag tctatttagc atttaaatga attaacacta tgaataaata tttgaaggaa
     3241 gatattatga aggaaaaaat aaaaattatt attgcggcag atcttgtgcc tacacaatcc
     3301 aatattgaat attttaagac aaaaaacatg gacgctctaa tcggtcatga actcgtaaca
     3361 ttgttgaaat ctgcagatat acgaatattt aatcttgaag ctcctataac cgagagacgt
     3421 gacaagataa taaaatcagg accgcaccta aaaattgatc ctgtagccat taatggtata
     3481 atagagttaa atccgtcagt gttgtcgttg gcaaacaatc atatcaagga ttacggggag
     3541 aatggaatta gagatactat tgattatctt cggaataatg atattcccta tgtcggagtt
     3601 ggagagaata ttgataaagc acgtgaggga gttactataa ctataaaagg tcatattatt
     3661 tccgttattt ctttcgcgga acatgagttt tcgattgcac gtgataatca atgcggagct
     3721 aatccatacg atcctttgac ttcttttgac gatgtaaaga gaatagccga aagatccgat
     3781 tatgttatag tgctgtttca cggtggaaag gagcactatc gttatccaac agttgaacaa
     3841 aaacggatat gtaataaatt tttatcagtg ggcgcaaaca ttgttgtatg tcaacatagc
     3901 cattgtattg gggcagcaga aaacataaac ggtggagttg ctgtatacgg tcagggcaat
     3961 ttcattttta atctttgcga tgatgagcgt tggaaaagta gcctattgat tgaaatcgaa
     4021 ctgtgtgaat tatctagtat tagttacata ccaatagtaa aatgcggtgc aactattaga
     4081 attgcaaatg aagaagaagc aatacatata atagatggtt ttgagtcacg gtgtaaggaa
     4141 attttggaag aaggtttcct tgaaaagaat caaaaaatag cagccaagcg tgctgcgaat
     4201 tattatttga agaaacttca cggtgcaggt tttttcttcc gagttcttga taaaatactt
     4261 tcaggtagaa taattagata tatttatagg cattccagta taagagcaga gctgttgaat
     4321 tgtatagaat gtgagacaca tagagaattt attacggact atcttcgtaa ttcattaatt
     4381 gattagaatg gtgataatgt gaaatgaata aaaaagttgt aattattggt gatgaacact
     4441 acaacactct cggtgtcatc agaagtctag gtgaagcggg tgttcggcct gatattatta
     4501 ttattgattc atgtaaagca aaaagttggg ttctgaccag ttgttacgta aaacatggta
     4561 ctattgtcga acgcaacaat gaaaaaatta tagctgttct tttggataaa tcaaatggcc
     4621 aaaacgaaag aatatatctt gtccctacaa gtgactttgc agttaagctt attgatgata
     4681 attttaatgt gctaagagag cattatgttt tgccgagtat tgggaataaa gctggagaat
     4741 taactcggct tatgaacaaa agagttatgg cacaatatgc agaggatgct ggatttttga
     4801 tacctaaaac taaaagtcat tttgtattgc ctgattttaa aaatgataaa gagttattcg
     4861 aaagcatcaa tgttttatat ccagttatcg taaaacaaga aaatgctttt gcggctaaag
     4921 ataagatatg gatagtgaaa aacaaaattg aattaaaatc tgcactttct aagtgtataa
     4981 atgaaaaagt cattattcaa caatttatag aaaaggatga agaaattggc atacaaggtg
     5041 taggttttgg cagctctgct gagccagttg ttccaggtgt tgtacataaa ataagaacat
     5101 ctttaaatgc tattggaagc acgacatatg ctgatttacg gagcatagcc caaaatgatc
     5161 cagttcaaca ggttaagaac ttcataaaaa acatcggata ttatggtatt tttgacattg
     5221 aggtattgcg gaagggtgaa agttactatt ttatagagtg caatttcaga aacggagctt
     5281 atgggtattc atacacacgt atgggagcaa atcttcctat gatatggatg ggggagaatg
     5341 taggagtgaa tacatttaat tatacctcat tgaaattaat aaataccttc agcgactaca
     5401 agcatattaa aaacggcaat atttcttggt ataagtggat gtggcagttg gcaacagcta
     5461 atgtttatct tacagcaaac tgtaaagatc ccaaaccatt tttttataga ctaatcatga
     5521 ggtgaaaata aaaatgtatt taattcgttt agatgacgca tgtccatatt acgatgtaga
     5581 aaagtggcaa catgttgaag gcatactaga catgtataaa attaaaccga ttgtggctgt
     5641 gattcccaaa tgtcaagata aaatgtttgt caatcaatat caatacaacg atttgttctg
     5701 ggacaaggca cgacactggc aggataaggg ttggacgatt gcgctacacg ggtataacca
     5761 tgtttataca aatatgaatg caaagggatt gaatccgata aataattttt cagaattcgt
     5821 tggattaagt tatgaggagc aatgcgaaaa aatatataat ggagtgaaaa tcatgaatga
     5881 tcatggttta catccgaaat gttttgtagc tcccgcacac agctttgata caaatacttt
     5941 aatagcgtta aagaatgtgt catgtatcag ggtaataagt gatacagttg ctaatgatat
     6001 atattacaag catgaaatgc tttttatacc tcaacaatgc ggcagtttca ttcgaatgcc
     6061 attgaaaatt ataacttgct gctaccatcc taatgccatg caggctaagg atatacatgc
     6121 actcgaaaat gttttagcta aatataaaga taaatttacg aactttgatt ctcttaaatt
     6181 taagaaaaga aatttcggat tctttgatta tatgctgaaa aatatatact ttgctcgcag
     6241 aaagatgaaa agaattaaca tacattattg aaaaggactg atcttttatg aaaataaagc
     6301 aagtgaagct taaagcactg gaggatacat ggaaaaaaat gtattcttct aataatttgc
     6361 tctttcctta ttcatcatac gaatttgctt cttgttataa caaatacttt catctaggaa
     6421 gtaaacgctt gttcatgaag caattatttt ttttgatatc taatgatgat aaggatgtgg
     6481 caataatacc tctgatttgt agaaaagacg attattattt atatggagat atgtgtgcaa
     6541 caggttatct tgacatcata acagctggcg aaatcacaga tgatgaatac agcaagattt
     6601 ttgaattaat tttttcaaaa attggcaatg ctacattgaa cttaaacaaa attaatgacc
     6661 gtagtcgttt atttcattat ttattgaagt attatacaga ggaatactct tcaccttgtg
     6721 tcaatatctc ttttagtaat gattatgata catatatgaa gtcactaagt aagagcgtac
     6781 ggcaaaatat aagaactgcg tataaccgta ttcaacgtga aaatcgtaca tgtaatttaa
     6841 aagtcattat aaatgagtca ctaccaagcg aaattataaa tgagggaatg aagatttata
     6901 ataaacgtga ggagaacaga aataaaaaaa agagttcatt agtaactaaa attatcagaa
     6961 gacactttaa tccgattact acatcaacat tgtttatacc taataatatt catgcgttgt
     7021 tttttattga tggtgatatt gctgcatttt tgtcaggata tataacaaat gataataaga
     7081 caatagtagt acctcgtcac gcaataaata ttaactattc ttcatatagt gcgggaacat
     7141 tacttataac tgaacttata cgatattgta ttgataatac agaaatcagg aatcttgacc
     7201 tttccagagg tgatgagaaa tataaatatt cattgggtgg tacaactcat tacaaccatt
     7261 catttgtttt gaaacatcag agagagggga agaaataaat gaaaaaggta atatatctaa
     7321 ccacatcttt attccctgag gggcaggcgt attcagtgcg tgttatagga tttgcagctt
     7381 tgtttaaatc attgggatat tctgtacatg tgattgctga cagtacaaga caaaccgata
     7441 ttgtcaggaa caaaatatat acgtttgatt acggcacata tgagatttca ggaaagaatg
     7501 gacgcttaag tagtaatctt acaaacaagt attttgcaat tgccgctttg aaaaaacaat
     7561 tatctaatag cgatgcttgc gctgttgtag caaatgcgtc aagtgacagg ttcaatgatg
     7621 ttttattatt gtgccataaa aacggagtca aactaatatt agaagtttgt gaatggtatg
     7681 atgttatatc atcgcgtctt ggcttgattg attaccgcaa ttatcggatg aatcggatga
     7741 tggaaaaggg ttattctaag gcagacagca taattgcaat cagtaggtat ctcgaacagc
     7801 atttcagtaa aaggaacaac atagtcataa gaataccaac aatttttgat actcagcaat
     7861 tattgtatac catcgattcc aataaccaaa agattgttct agtgcacgca ggaagttctg
     7921 ttatacataa agaaaaattt aaacatatta tgatggcaat tcaaaaaatt ggaaaggata
     7981 caccgattga atatagaata tatggatcta gccgagatat tgtcctgaaa aatatagacg
     8041 gtgatgacgc gttgctgcat gctttgggag aacaggtcaa aataatggga acaatcccac
     8101 aagctgaagt aaataaggca tatcagcaag cggatttttc aatatttatg cgtgacagaa
     8161 aatgttctac atcagctgga tttccaacca agcttgcaga aagcatggca gctggaacac
     8221 cagtaatagc aaatgacact ggagatatag gcttatattt gaaagacgga tacaatgggt
     8281 ttttgataaa cgacaattct atcgaagaaa tcgaatccac gctcagaaaa gtgatttcac
     8341 tgtcttcgaa gcagaagaaa aatatgcgtg aaaatgctcg taagacagca gaagcaagtt
     8401 ttgattatag aagttatata gatatcgtta aggagttatt caaagattaa tttattaaaa
     8461 tgatttactt gaacaatgaa aggaaatgat atgacatgaa atttatcaaa ttaataaaaa
     8521 ttattataga taagtatcag caagccagca accctgtaaa gtacgcaaaa aaaatcggtg
     8581 ttaacataaa gggacgggtt catttatatg gaaaacagat atggagtaca gaaccgtgga
     8641 ttatcacttt gggtgataat gtttatttga caaacggaac gcgctttatc acacatgatg
     8701 gaggcacatt aatattaaga gataaggttc ctgatcttga aatcacaaag ccaattacaa
     8761 taggaaataa tgtatatgta ggggttaata ctacattcct tccgggggta aatatcggca
     8821 ataatgtgat tataggggca tgttcagttg ttacgaaaga tatacgtgat aattcagttg
     8881 cggcaggtaa tcctgctcgt gttatcaaaa ctattgatga gtattttgaa aaagttcagg
     8941 cagaatcgtt gcatctcgga cacctgaagg gtaaagaaaa agatattgca ttgcgtaggt
     9001 attataaata tgataaataa agtcttgagt attgttaaaa aatctgtcat gaaacttttc
     9061 gacaaagata agctgaaagt atatgtttac actgcttcta acattgctac aatgctgatg
     9121 gcatttgcta tatcagttct tctaacaaga tggttaaatc ctgaaatata cggtaaatac
     9181 aaatatgcag taaacattct ttttacctta cctacatatt ttgaatttgg cattcacttc
     9241 tgctcaagcc ggattgttgc cgaggataat gctgatgaaa gtcacggtgt tgttccagta
     9301 tcaattgggg tactgtctgc attagcagtg ataataactt tcggattgta tatgttcatt
     9361 ttcacatgta aaagctttga aatatataca ggtatagaaa agctcaatga tgtaagaatc
     9421 atttttccat ttattatcgt atttgcaagt aatattgttg ttacacagct ttaccagggt
     9481 accgggaaaa tatacaggct atccgcatat tcctttattc aatatgctat agttattgtg
     9541 ggcgttgttg taggtcatta tgcaactttg caactgacgt ttgaatattg catttttgtg
     9601 tatataatat cgaatgctgt agttcagatg cccgtactaa tgagaatccg tattcagagg
     9661 gatttgatta taccaaactt caaaagattg tttcatgata taaaatcctt tggtattaag
     9721 atatatcttt cgtcttttgc gacaagcgga gcttccacat tgatagggct tatatgtggc
     9781 tctatatacg ggtattcaga atatggttat tatactttgg ccatttctct cgctcagttt
     9841 tttactttca tttcatcgtc tatggctact gtgaaattta aagataatgt aaaaggaaaa
     9901 tatctgaaaa agagcgacat gttgttcatg ctatttatga atagcttaat atacttagtg
     9961 tttagtataa tgataaacaa agtgtttttc tggttttact ccccagagta tgctaagtct
    10021 attgcatacc ttagaattct tgcgatttca tatattttta atggcatggc tttattttat
    10081 aacaggtttt ttatcgcaag aaaaatggga aacataataa tgaggaattc ttttatggta
    10141 gcagctgtta atgtcattgc ttcgattatc cttattccgt tgctgaaaat aaatggtctt
    10201 gttatagctt ctcttattgc atcagcctat aatttttttc aatatattat tacatacaga
    10261 tcatacttaa agaacttaaa gaatgggaaa aaggcggttt tagataatga ataatttcaa
    10321 tacattgaca tacgcaaata aaaataacat tatagtgtca tcaaaaatca gtaaacaaag
    10381 agataatacc gttttacaat taatattatt ttatgtgttt ataatagttg ctataatact
    10441 aaccgacaag ggaataagtt tagctagagt gattgcattc tggggcatat tgtttattgc
    10501 tgtccttcag aaacctatca gcatatttat gtttatggtt gcaacattgc cattgtcaca
    10561 tattgctagg atggagaatt ctagtttaac acttcttccg ttcttaacaa ttattttatt
    10621 aataaagata ataattacaa ataaagcaat atcactcaat aaagcaatgc cgttgtttat
    10681 tattggaatt atcagtataa tcaacgagat cattcgatgg tcatcgatga ttacttcttt
    10741 ggtttttatt atgacatggc ttatacctgt atttgcctat tgtataaagc aagagataaa
    10801 tcataaaatt gtgttaatat cgtttgctta tgtaatcagt tccttcatca gtataattgg
    10861 aagtactcta ttcccaaatg cagcacgtat gataacctct tatacacaat actcttatag
    10921 agctgtcggt ttctcgcaag cttggtcata tggtattaat cttgtaattg cgtcagcttt
    10981 tatattaata atatataaac aaacaaataa gcgtttagtt ttaagtgcaa ttgctattgc
    11041 tatattattg tataatgcta tacttagtgg tacattttct atattaatag gtatcgcttt
    11101 ggttttgttt tgtttcagta tacaaacact taaaaaagag aaagttctgt tactgctcct
    11161 gataccactt ctttttgtct taggatatag cattatgaaa gcattctttc aaattagagg
    11221 cgatataagt gataatgggc gtatagagat atggatgttc tatatgaaac atttattaag
    11281 caattttagc gttttcatgt ttggaatagg cggtggtaat attgcaagta tggcttcgaa
    11341 gtatcaaatg tctactgctc acaatggtat tttagaatta ctcattgaag tgggattttg
    11401 tggtacagtt ctcttgattt tatattcaaa gaacctgtta agaaatataa aacttagttt
    11461 ccgtaaaaac atactttcgc ttcctttttt gatatacttg tcgtttttgc tgactcaggg
    11521 cggagcttca tcatattcaa tagttatttt tatatcatta ctaataagtt catcagaaca
    11581 aacaaagaat caaggagtag aacaatatgt ttgtaaataa aaccttacta atcactggtg
    11641 gtaccggttc attcggcaat gctgtactga agcgtttttt aaatactgat atagggcaaa
    11701 tacgcatttt ctcccgtgat gaaaagaaac aggatgatat gcgaaggctg tataaaaatg
    11761 ataaaattaa atattatatt ggtgatgttc gggacattgc aagtgtcaag aacgctatgc
    11821 atggagtaga ttatattttt catgcagctg cattaaaaca agttccatca tgtgagttct
    11881 ttccacttga agcagttaag acaaatgttt taggcacgga caatgtactt acagcagcta
    11941 ttgagtgtgg tgtcaaaaaa gttatttgtc tatctacgga taaggctgct taccctataa
    12001 atgcaatggg catatcgaaa gcaatgatgg agaaggtagt tatagctaaa tcaaaaactg
    12061 tagatccaga taaaacctta atatgttgca ccaggtatgg taatgttatg gcttctagag
    12121 gttctgtaat acctttgttc gttgatcaaa taaagagtgg acaagcttta actgttacta
    12181 atcctagtat gacaagattt ttaatgagtc ttgaagaagc tgtagagctt gtggtatttg
    12241 cattccaaaa tgcacagtca ggcgatatta tggttcaaaa agcaccggca tctactatag
    12301 gggatttggc tcaagcgata aaggagttat ttaatgcgga taatgaaatt aaaataatcg
    12361 gtactcgtca tggcgaaaaa ttatatgaaa ccttattaac aaaagaagaa tatatagctg
    12421 ctagggattt aggtaattac tatagagtac cggctgataa aagagatctt aattatgaca
    12481 aatattttat tgaaggaagt caacagctat catcaaatgg agaatataca tcccataata
    12541 ctaaaagact taatgttgaa cagattaaag agaaactatt gactcttgac tatatttaaa
    12601 aaagaactgg aaggttggag gaaataatga aaatacttgt aaccggtgca aaaggattta
    12661 taggaagaaa tcttgttgca gaattaaaaa atcgaaagca taccaatatc tttgaatacg
    12721 atatagatac agatctatct ttattagatg catattgcag agaggctagt tttgtatttc
    12781 atttagcagg tgtaaatcgt cctaaagacc aatcagaatt tatgaaaggt aattttggat
    12841 ttacttcagt cttgctggat actatgaaaa aacataataa cacatgtcca gttatgattt
    12901 catcttcaat tcaggcagcc cttgataatc catatggaaa gagtaaaaaa gctggggaag
    12961 agttaatgtt tgcatatggt aaggaaacag gtgctagaat attagtttac cgttttccca
    13021 atgtgttcgg aaaatggtgc agacctaact acaatagtgc aattgctaca ttttgtaaca
    13081 atattgcgca tgatttgcct attaaggtaa atgaccgcaa tgttgttatg aaattggttt
    13141 atatagatga tgtggtgcag gaacttataa atgctttaaa tggtacagaa aatcgtatag
    13201 gggatttttg tgaagttcct gtagttcata ctatcactct tggtgagata gtagacttaa
    13261 tatgttcctt taagaatagt cgtgaagaac gttctattcc agatatgtct gatgaattta
    13321 cgaagaagtt atacagtaca tatttaagtt atctgcctac aaagcagttc agctatccat
    13381 tgaaaatgaa tgtagataac agaggatctt ttactgagtt tataaagact ccggatagag
    13441 gacaggtatc agtcaatatc tcaaaaccag gcatcattaa aggaaatcat tggcaccaca
    13501 ctaagaatga aaaattcttg gttgttagtg gtaaaggtgt tatacgtttt agaaaagttg
    13561 atacagatga aatcatagaa tattatgtta gtggtgataa gctggaagtg gttgatatac
    13621 caactggata tactcataat atagaaaatt taggagatac agatatggta actataatgt
    13681 gggcgaatga atgtttcaat cctgataagc cagacactat tttcttggag gtgtaaatgt
    13741 ggcgaataca gtattaagga aactaaaatt aatgacaata cttggtacaa gaccggaaat
    13801 aattcgtttg tctgaagtaa ttaaaaaatg tgataagtat tttgaacata ttctggttca
    13861 cactggtcag aattgggatt atacgcttaa tcaaatattc tttgaagatt taggactaag
    13921 gaagccggat tattatctgg atgcagttgg tgtggatttg ggtgaaacta ttggaaatat
    13981 tatagcaaag agttataagg tactttcaga agtaaaacct gatgcacttt tagtcttggg
    14041 tgatactaat tcagctttat ctgccatatc agccaaaaga cttaaaatac ctatattcca
    14101 tatggaagca ggtaacaggt gttttgatga aaatttgcct gaagaaacta atcgcaggat
    14161 tgttgaccat atatctgatg tgaatttatg ttacagtgaa catgcaagaa ggtatttgaa
    14221 ctttgaaggt gtagtaaagg aaagaaccta tgtgactggt tctcccatgg ctgaagtgtt
    14281 aacagctaat atagaaaaaa taaggaacag caagatatta gaagagttag gattggaaaa
    14341 gggtaaatat attttacttt ctgctcatcg cgaagagaat attgatattg aagaaaactt
    14401 ctttgctttg atgaatgcag taaatgctat ggcagaaaac tataatatgc ctgttatata
    14461 cagcactcat ccaagaagtg caaagttcat tgaacagagg cggttcaaat tccatccaaa
    14521 tgtacgaagc ttaaaaccat ttggattttc tgactataat aatttgcaga taaatgcatt
    14581 ctgtgtagtt tccgatagtg ggacaatacc ggaagaagca tcctatttca aatttcctgc
    14641 tgtgtcagta cgtacaagca ctgaacggcc ggaatctatg gacaaaggta attttattat
    14701 tggaagtata actacagagc aggtgctgca ggcagttgac cttgctgttg ctatgtatga
    14761 aaatggagac cttggtgtta caacttctga ctatgcagat gaaaatgtga gtacgaaggt
    14821 ggtaaaaatt atacagagct atacaggaat agttaatagg atggtttgga ggaagtagaa
    14881 cattgctgtg cttagaaaac atggtaaaac ttgcagaaag taaaatggat tttaatatta
    14941 tggcttattg ctaaaatata cgccttatgg actcgccata agggaattat attacgtttg
    15001 tcaactgtaa agtaattatt acaattccac aaaggttgtc aaacaggctg aagtttatat
    15061 tgactattaa caatatcatt gtaaacattg gtcgctcctt tctattatac cataaataaa
    15121 tgaatggtgg agcaaataag cagcttggta ttacttgatc tttatggtca agaatatcaa
    15181 agtttcattg tgtgcctacc gaataaagga tgctgcttcc tatcggtggg catctcatta
    15241 aagggagaga tataggtata aaaaacaata aaatatacaa aatgaagtat tactcacatt
    15301 ttgacgagaa agtacattgg aaaaatgtaa aacatatcgt tgaaaatcca gaaaaggtgg
    15361 caagacatgg gttcttccca tttattcact atactcataa tattgagaag ttcaataaaa
    15421 aggttggaag gaagccacca aaattaagag agatatttta ttcttcgcat attgatagat
    15481 atatttatga attatatgcc tataagctta ataatattta taacaaaaga gttataaaag
    15541 attgtaccaa taaatgtgct gtagcatatc gcgataattt acataaaagc aatatacatt
    15601 ttgctaaaaa agtatttgat gcaattcgta gatataatca agcttacgtt attattggag
    15661 attttacagg attttttgat aatcttgatc attcatattt gaaagacaga ttgtgtagtt
    15721 tgctagaaac cactatactt ccagacgatt attatgcagt attcaggaat attactaaat
    15781 attcatattt tgaacttgaa aaactcaagg aaattaccgg attaaaatac aaagaactaa
    15841 acaagcaaaa cagagtgttg gaattaaaag attttaggca atataaaagg gaatgtttac
    15901 gaaagaacaa aaacaattat ggaataccac aaggatcggc tataagtgct gtattatcta
    15961 acatttatat gattgatttt gataaaaaga taaatgattt tgtcacgtca aaaaaaggaa
    16021 tatacatgag atatagtgat gattttgtaa ttgtaattcc taaatgtgag attgttgagt
    16081 ttcaaaaaat gtgggtattt attgataata tcaaaagatc tgtaaaaaac ttagagttac
    16141 agcttgaaaa aactcaaata tttgagtata acaatttaca cgtaaaaagc tgtaacagtt
    16201 tgctttttca tgaagtacct gatggaaaaa atattataaa ttatttaggc ttttcatttg
    16261 atggaaaggt tatcaccatt cgtgacaaga ctatttctaa atattattat agaatgtaca
    16321 ggaaaattga tgctatagcc aaaaaaggtg ggtatacaaa aaaaggaaag aaaattccaa
    16381 tgaatactct ttataaaaga tactcttttt atggagaaga gcaaactaaa gataatagag
    16441 gaaacttttt aacatatgtg aatagagcaa aagaagtttt tggtccaaat gaggcaattg
    16501 accgtagtac taaaaagcac tggggtaaat tgcagaaacg cttacataaa ttttggaaag
    16561 agcatagaga gaactgagag aaaatagctt tgaagatagc tatatttatt attaaagaaa
    16621 aaagttaaag aatgttaagg caaggattaa taattatagt taaaatatat tgaaaacaaa
    16681 gaaaaaagtc attttttata tagacaataa atttataata tgatattcta tatttaaaca
    16741 aagaacatat gttttaaacg ctgattatca tattttgtga agtatatgat atatatttaa
    16801 tactataagc ttaatatttg tgaaggtact aaaaatatac taaaaaccaa atgataaaag
    16861 agaaaataca gtgtgaaact gctatatgag aatgcaaatg caaaaccgat taatcaaatt
    16921 atttcccttt aacatttacg tgggagttgg tgaggcatgc aaaaaatatt cagagatcct
    16981 atctatagac taatatcatt tgataaagat aaggatagtt tattattaaa attaataaat
    17041 acttatgaat ttcaaagatt aagaagaata aggcaattgg gagtgtcttg gttaacctat
    17101 cctacagcag tgcatacacg tttttctcat tctataggtg tagcttattt agcgggcagg
    17161 atatttgata aattatcact agaagatgaa attaagtttg atgacgatga tgataattat
    17221 gttttatcca aaagtgaatt gaaattgctc ctacaaacaa cagctttatt acatgacata
    17281 ggacatggac cgttttctca tgcttttgaa gatgtgatta gtcagaaaca tgaagaatgg
    17341 tcaagaaaga taattcaaga tgaaaataca aatattaaca aaataatttt gtatgatgag
    17401 gaaattagta attatattag tcccaaaaac agaaaaaagt ttcctcagtg gattagcaat
    17461 atattaaatg gtatattccc tttatattac ataaatgaaa taatttccag ccaacttgat
    17521 gcagataggc ttgattattt attaagagat gcctatatgt gtggggttga ttatgctaaa
    17581 tttgatctgg aatggatatt gaataatgtt aatgtaggta taataacgca tgaggaaggt
    17641 cgaaaaggga ttgtcattaa tgctgaaaag ggtatatatt cattagagtc tttcattatt
    17701 tcccggtacc atatgtatga acaggtttat tttcacaaga ctactcgagc agcagaaaaa
    17761 ctaatacata atatttttat aagacttaaa gagctcattg gtgaaggaaa aacagaaaag
    17821 ataggacaga ttgataatag tatattagct gtattcactg gcaatattaa tatagaaaat
    17881 tatctaaagc ttgatgattt tatgttgata tcatatatta ataagtgggc tagtgaaagt
    17941 aaagatgatt tattaaaata tttatgcagt aattttatca acagaagatt gtataaactt
    18001 gttacagagg gcgaaaatgt ccaaataatc tctagcgaag aaagtgataa aatatatgaa
    18061 ttttttaaag ataaaaaatt agtaaaaaag tattattttg ttgaagacag ttatactgaa
    18121 gttgcttata aggatgatta cctttttggg aaaaagagca gtgaagatgc agcgcacata
    18181 tggcttattg atagatttgg taatttggtt gatttaggag aagcttcagc aattattaat
    18241 gctttaagaa ataacataaa gaaaaaatat aggatttatt gcgatagaag tgttgtccag
    18301 gaattttttg caaaaaaatt tttaggaggt atgtgatatg aatgaatcat ttaaaaaatt
    18361 gttacagttt gttggtgtta
//
LOCUS       DAOSDD010000067        18025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1264_length_18025_cov_4.909738, whole genome shotgun sequence.
ACCESSION   DAOSDD010000067 DAOSDD010000000
VERSION     DAOSDD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18025
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_18025_cov_4.909738"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PK733_07860"
     CDS             complement(<1..1084)
                     /locus_tag="PK733_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ10492.1"
                     /translation="MKPLNLADLTIEQKIGQMLLCRRPLNDEDKEYILELIRNRSLGG
                     IHGMFPELLETADYPLLICENMESGFPKGEAELTCPLAIGATGSEEYAYEFGRISAIE
                     AKAKGYNVVFGPIFDIALNPAAFCGGSLRFGGNKELVSRLGAAAVRGYQDQGMIVTAK
                     HYPGFGESAIDSHLEMVYLKCDKKLLLERELEPYRYAIEHADLSGVMVGHITVPKIDD
                     KYPASLSPKITNILREIGFDGLTMTDSLAMLGITNYYGLEECHALAMTAVDMVMASYR
                     VPAKMAYEFMLNAYRKGRVTEEQIDEAVRRVIAAQNKTLKKPETTKLTHKEMNLAKEI
                     SRDSITALLDGVDSTAIPTDGRHLFIL"
     gene            complement(1144..2499)
                     /locus_tag="PK733_07865"
     CDS             complement(1144..2499)
                     /locus_tag="PK733_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10493.1"
                     /translation="MNIKVLLWLDVEDYINLQSDDALLLLLDTLKSKGVKAILKIVGE
                     KARVLKERGRYDILEKIKEHEIGYHTNMHSVHPTIAEYLEPLGFREGAYEFERNEGNG
                     FKDVASIISKNPEVYGQPGRDWAPQVFPVLRKWLVHSYICTVRRINIARKPCWYGGLL
                     SMCYLNGQTRMELVEGGLEEGKRTFDETCKRLSDQNTGFVSIFYHPCEFATTNFWDAY
                     NFSNGENTPRNEWKGAPLRSKEEMIHYVDMFGQYIDYILSKDNVEFITLSEALALEKS
                     NNTGICAEDVKIIAEEIGTEVYFYSNNNINLSASEIFSLFCNYINDEPFNPELIYGPE
                     NYFTTNITEKVKVSDIKQAVKVKYPRIYDYKQLPDYFTINGKYINPIDMTCTLAKIIK
                     DGLTDNDSVEVVRGCLKSRIHVEEKDDLTNWIVFRKNFQVPNIRKMAGLQAWTLKPAL
                     F"
     gene            complement(2532..3434)
                     /locus_tag="PK733_07870"
     CDS             complement(2532..3434)
                     /locus_tag="PK733_07870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10494.1"
                     /translation="MIKKIDIHAHSVRIKGINFYKSNKTFATPYEVAEIYERIGVEKG
                     VLLPLVNPECAGMIQGNEDAMEIVEQYPDLFYWFCNIDPRMGQNSTKTDLSFYLEYYK
                     ERGAKGVGEIEANMYFDDPFVENLFYHCQKNQMPVLFHMATKIGGSYGLVDEVGLPRL
                     EKELAKFPNLIFIGHSPSFWSHISDDLTEENWEDWYYPKTKVISDGSIVKLMRKYPNL
                     YGEISAGSGNNAMLRDPEFSYDFLEEFQDRILFGTDICDKNYQLNTSGWLDEAVQNGK
                     ITNKTYEKVSRENALKILKEEEKL"
     gene            complement(3456..4403)
                     /locus_tag="PK733_07875"
     CDS             complement(3456..4403)
                     /locus_tag="PK733_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10495.1"
                     /translation="MNNRIKNTLDIDNKISEIHLQKIDIHAHAVLTKGINYFKSKITL
                     ATADEVVEIYKRIGVEKGVLLPLVNPECRSRVQGNEEALELVKSYPDSFYWFCNIDPR
                     MGQNSTKTDLSFYLEYYKERGAKGVGEVAANMYFDDPFMDNFFYHCQKNQMPVLFHMA
                     TQIGESYGLVDEVGLPRLEKELAKFPDLVFIGHAPPFWSHISNDLTAENWTNWYYPKT
                     KVIPDGSIVKLMRKYPNLYGDISAGSGSNAMLRDPEFSYDFLEEFQDQILYGTDISCK
                     EFNLKISEWLDEAVENGKISYKAYEKISRGNALKLLKEE"
     gene            complement(4453..5343)
                     /locus_tag="PK733_07880"
     CDS             complement(4453..5343)
                     /locus_tag="PK733_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10496.1"
                     /translation="MEKIDIHVHAVPRRGINFYKSDKTIATPEEIIEIYKKTGVGKGV
                     IMPLRNCEFGNIFQGNEEVCIEIVNKYPDYFYWFCNIDPRNGDNTPQSDLSFYLEYYK
                     DMGAKGVGEIGTNMYFDDPFVENLFYHCQKNNMPVLFHMSTQIGESYGLVDEVGLPKL
                     EKELAKFPDLILIGHSPSFWSHISDDLTAENWKDWYYPKTKVIQDGSLVKLMRKYPNL
                     YGDISAASGNNALLRDPEFSYDFLEEFQDQILFGTDISHKDFYLNTSSWLDEAVENGK
                     ISYKAYEKISRGNALKFLES"
     gene            complement(5612..8053)
                     /locus_tag="PK733_07885"
     CDS             complement(5612..8053)
                     /locus_tag="PK733_07885"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10497.1"
                     /translation="MDIKHGINNIFNKRSINLSLKIFIVCISISLLLTIIVGIFTHNY
                     VMQQFDREIEKFEKGQVEKTANSIKMRFKEFENMGLYYASHPYFNQFVSLTRSEVILD
                     YRTIKKLMDILSGVINSSNYINDIFVYYGKSEVVLNYTGIYAPHFFYETGWEKYYEEM
                     TGNCKIIDTRSVDNSKKFRRGNYTDYNNNVITFFIGIPYDNEKKLGTIVINLNYEIVS
                     DILKNITNNSQSLAFLINEAGIVLSSNHDEYLYKDINEITFGQIHFKDEKLGKTDINL
                     ADCKMVCYYSDVDINGWKLCIMIPSNIIFKKSLEIRLIIVLVLITILIIIIIVSLLLS
                     KKIYKPIKEITLLLKSETNHKNDDSTNNNTNNNTSNDAEEINEADEICDTRNKYYNSD
                     IFIIKNGLKTLLQDRKDLEKVLDENRLLFREYFISNLITGKIRTREFITKKAEYFNIH
                     INCKYYRVATIKNISIYPGDLDIEEFEVKKMAIINIVQAAFKNNGIDVICSQDTDDNI
                     LILIKIYTETVKEQLDSLISKCMQTIKNNIEHYLSSQVILGIGKMHEDVLDIELSYKE
                     SLKALKYSFIMGDIVDAYNINYSEDSKLFYPIEIEKNIISFIDLCDYENVILCINNGI
                     KNIIDANKNIDHIFICFSNLINLVNRCMFEFNINPKNIMPKETGKNISINDFKNIEQF
                     MKWLSIVFKKVVEQHIYIRNESEIDFSAKIRQYIEKSYMSDITLSSVSDHFGYNSSYF
                     SRLFKEIMGANFIDYINQIRIEKAKVLLATTNISTQEIAEQVGFNNRISFVRSFKKFT
                     TVNPGEYRGKKRNAW"
     gene            complement(8393..9772)
                     /locus_tag="PK733_07890"
     CDS             complement(8393..9772)
                     /locus_tag="PK733_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ10498.1"
                     /translation="MIMSDYMSDYKELLESVDMYRDLIFAAEQYIWEHPETGYREWKT
                     SAYLEAEFERLGYNLVKAGNIPGFYTTLDTGRPGPCVLIMGELDSLICADHPDADPKT
                     GAVHACGHHAQCAALIGLAASLKEDGALDGLSGSIRLCAVPAEELIETGYRETLREAG
                     IIRYFGGKVEFLYRGYFDDVDMAFMIHTKVGKDHFFAYRGSNGCIVKNINYKGVASHA
                     GGSPHEGVNALYAANLGIQAINALRETFRDSDHIRVHPIITRGGSAVNAIPNDVAMES
                     YVRGADIKVVSEINKKVNRALAGAAASIGAGVFLSDRPGYTPLINDKNLLHIAEKAMK
                     IIVPEGNIAISDSWNTGCTDMGDISAVMPAIHPYVGGAAGNSHGSNYHIEDHETACIN
                     SAKCQLVMLHTLLSNDAAEAKRVISEKDLRYASKTEYFKDIDALTQDKNAVIYKEDGT
                     VILDYTKSL"
     gene            complement(9805..10575)
                     /locus_tag="PK733_07895"
     CDS             complement(9805..10575)
                     /locus_tag="PK733_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10499.1"
                     /translation="MYNINKIIDFSTWTGNWPFIHLKNSGLDDLKKKLKSQNVIKAFV
                     SPIESILERDPMRADKQLLQDIKDDFFSPVPVVDLSYANWEKVVELAIQDKRVKMIKL
                     IPNYHIYELSEEKLEKLVNLTMYNKLIIAIQMRIEDTRGQYPLMKIPDVDMLSTIRAL
                     SNFPQQAFILNNVYWGEVAPALHSTSNVYIDISSVECPNVLHTIRDAYSLDKILFSSH
                     SAFYYPEGNVFKLNYSDLDVSEIEKVAYRNGEILLNSL"
     gene            complement(10568..11350)
                     /locus_tag="PK733_07900"
     CDS             complement(10568..11350)
                     /locus_tag="PK733_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10500.1"
                     /translation="MIFDSHLHVTLVGEKNTEGAVQKLLDSMKLVGVERSCVMPEPVS
                     SLYFSKDDMTGQAAILDDIGQKHKDILYPLLWINPTLPFDFTKELIDRYIINGNIIGV
                     KLWIEINARNKRLEPLAEYLEKHDIPVLFHSWYKTVSKYGAESDPSDIAVLSRKFPKL
                     RILMAHLTGCRLRGVQDIKDCPNVWIDTSGGQPEDGYLEYAIAELGPNRVLFGSDYGG
                     RDIATQLGRIFSVDMSDEDREKILSLNALEFYRKGERVNRHV"
     gene            11612..11983
                     /locus_tag="PK733_07905"
     CDS             11612..11983
                     /locus_tag="PK733_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10501.1"
                     /translation="MQAIFETLFDTIYLIGVITVGIIMMKNAKSNKQTFLFGFMSVLL
                     GLGDSFHLVPRVYALWTTGLEANAAALGTGKLITSITMTIFYVILYHIWRERYKISNR
                     SSIIYSVYGMAICGLLLYIKR"
     gene            complement(12103..12972)
                     /locus_tag="PK733_07910"
     CDS             complement(12103..12972)
                     /locus_tag="PK733_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HOJ10502.1"
                     /translation="MIKKKNILILAFILITLLGVCFIGCGRNRQPAVLSTEKAKITEA
                     GKEREEEKAATKIKDAATEMKDISTEIKHADKQIKYELKTETFTYGKIAINYPQVVNM
                     TDSAVQNNINEIIKGNALKDKDELAKVTEEVTYELTYDVMYTSENLLSIKYEGYSFFK
                     GAAHPNNFIYTTNIDIEKQEVLKLKDFVNIDEDFVNIFKNGKYISMGDEEITSELKAA
                     LTEELNQTDTNGWINRLNNADTAGVDNYSGVYSCLTRDSLVISIGVPHFMGDYAVFKI
                     NYEDLSKHKKIKK"
     gene            complement(13344..13475)
                     /locus_tag="PK733_07915"
     CDS             complement(13344..13475)
                     /locus_tag="PK733_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10503.1"
                     /translation="MMTEQLDKWVDSYCEGFPFAMDPGFAISVCQCIPVERIPFSIK"
     gene            complement(13506..14948)
                     /locus_tag="PK733_07920"
     CDS             complement(13506..14948)
                     /locus_tag="PK733_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ10504.1"
                     /translation="MDMSSGSLFKKIIIFSIPLMISGVLQLAYNAADVIVVGRYAGSI
                     ALAAVGSTGSLINLIITAFMGLSVGVSVCVAQYYGAKDSENVSQVVHTAIGISVIFGI
                     ITSIIGISMAEVFLKAMGTPDEVLKYSVLYMRIYFMGMLATMVFNFGSSVLRAVGDTK
                     RPLMFLTISGLINVMLNLIFVIKFNMGVAGVAIATVVSQIISAVLVVLCLIRFEGSCR
                     LRLCDIKIHKDKLIKMMKIGIPAGLQGTIFSISNVLVQSSVNSFGQLAMAGNTASSNL
                     DGLIYMATNSFYHASLAFTGQNVGAKKYERIGKVLRICIFFVTIIGLSLGTLLYIFGE
                     TLISIYSPDNMDVVKYGMTRLRVMGLTYFTCGIMEVIVGSLRGMGYSLTPMLVSVAGV
                     CGIRIAWIYTIFAMNRTLEVLYLCYPASWIVTFSIHLICYAYVKKKLMRDAAHDFIDV
                     EIDIDNNADNVDNNIANDVDNNTINTDVLL"
     gene            complement(15272..15967)
                     /locus_tag="PK733_07925"
     CDS             complement(15272..15967)
                     /locus_tag="PK733_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ10505.1"
                     /translation="MLKAVIFDMDGIIIDSEPIYHKVEMSMFKELGLDLTDEHRYMYV
                     GIRTVDMWQELKQRYDIEFSVEKLVEIEAKRISEFLESQKKIKSIAGIKELLEELQNN
                     SIKIALASSSSIRDIETVLNKTGFREYFNVIVSGDYVKNGKPAPDIFELAVSQLNNMI
                     ETSTDASSYITPENCIVIEDAHSGVRAAKAAGIKCIAYRSPNSGNQDLSEADLIIDSF
                     YELSLNKMISLIR"
     gene            complement(16156..16881)
                     /locus_tag="PK733_07930"
     CDS             complement(16156..16881)
                     /locus_tag="PK733_07930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ10506.1"
                     /translation="MLTLKGKVAIVTGASRGIGKAISRSLAEFGVNLALNSRSLDQLE
                     AMKEEFKGLNIDVLSCPADLSDPEACVKIIEKVVQHFGGIDILINNAGIAVPKSLSET
                     TVNEWDMHMAINARAPFLLSREALPYLKKSDMATIINISSVVGYKGYINQGAYTASKH
                     ALMGMTKVLAQEVFKDNVRVHIIAPGGVATDMVTKTRPDLDESMLATPQEIADVVMFL
                     LTHRGNAVIDEINIRRFSNTPWK"
     gene            complement(17466..>18025)
                     /locus_tag="PK733_07935"
     CDS             complement(17466..>18025)
                     /locus_tag="PK733_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="spore gernimation protein GerMN"
                     /protein_id="HOJ10507.1"
                     /translation="LTTVIEFSLGEELKKPDAAEQIIKETADKLISAMSNKDAQTISE
                     YVHPDKGIRFTPYTSVSLEDDAVFNKQQMKNFFDDRNLYLWGHFDGSGEEIRLTPGEY
                     YDKFIYSEDFVNAKKIGYNEVLSNGNMIENQFEVYKNAIVVEYYFPGFNPEYAGMDWK
                     SLRLVFEQYEDGWKLVGIIHNQWTI"
BASE COUNT         5488 a         3577 c         2586 g         6374 t
ORIGIN      
        1 caagaataaa cagatgcctg ccgtcagttg gaatagctgt agaatctact ccatccagca
       61 gtgctgtaat tgaatctctg cttatctctt tagccaggtt catttcctta tgagtaagtt
      121 ttgtagtttc aggttttttc agagttttat tctgtgctgc tataactcgt cttacggctt
      181 catcaatttg ttcttctgtt actctgcctt ttctatatgc atttaacata aattcataag
      241 ccatttttgc aggaacacga tagcttgcca ttaccatgtc aacagcagtc attgctaatg
      301 catggcattc ttcaagtccg tagtagtttg taattccaag catggctaat gaatctgtca
      361 ttgtaagacc gtcaaagcct atttccctga ggatattagt aattttgggt gataagctgg
      421 caggatattt atcatcaatt ttcgggactg ttatatgtcc aaccattact cctgaaagat
      481 ccgcatgttc tattgcgtat ctataaggtt ctaattccct ttcaagaagt aacttcttat
      541 cacatttaag atataccatt tccaaatgag agtctatggc ggattctcca aaccctggat
      601 aatgtttagc agtaacaatc ataccctgat cctggtaacc tcttacagca gccgctccca
      661 gcctggaaac caattcttta tttcctccga atctcaaaga acctccacag aaagcagccg
      721 gatttaaagc tatatcgaat ataggtccaa aaacaacatt atatccttta gcctttgctt
      781 ctattgctga tattctccca aactcatatg catactcttc tgagcctgta gcacctattg
      841 caagaggaca tgtaagttct gcttctcctt ttggaaaacc cgattccata ttttcgcata
      901 tgagtaaagg gtagtctgcc gtctcaagca attcaggaaa cattccgtgg attccaccta
      961 aactcctatt gcggataagt tctaatatgt actccttatc ttcatcattt agcggtcttc
     1021 tgcaaagaag catctgccca attttttgtt caattgttaa atctgctaaa ttcaatggtt
     1081 tcatatcaat acctccgaat tctataaaat tccttcttat ctatctttat atgttcatta
     1141 gttttaaaac aatgcaggct ttaaagtcca tgcttgaagt cctgccatct ttctaatatt
     1201 cggaacctgg aaatttttcc ggaatacaat ccaattggtc aaatcatctt tttcttctac
     1261 atgaattctt gatttaagac atcctctcac aacttccaca gaatcattat ctgtcaatcc
     1321 atccttaatt atctttgcaa gcgtacaagt catatctata ggattaatat atttcccatt
     1381 aattgtaaag taatccggaa gctgcttgta atcatatatt cttgggtatt taacttttac
     1441 agcctgcttt atatctgata cttttacctt ttctgttata ttggttgtaa agtaattttc
     1501 cgggccatat ataagttcag ggttaaaagg ttcatcattt atgtagttgc agaataaaga
     1561 aaatatttct gatgcagaca ggtttatatt attgtttgaa tagaaatata cttcagtacc
     1621 tatttcctct gcaataattt taacatcttc cgcacatatt ccggtgttat tggacttttc
     1681 taaagcaagc gcttctgata atgtgataaa ttctacattg tcttttgata gtatataatc
     1741 aatatactgt ccgaacatat caacataatg tatcatttct tcttttgagc ggagaggcgc
     1801 tcctttccat tcatttcttg gagtattctc accattgctg aagttatatg catcccagaa
     1861 attagtagta gcaaattcgc atgggtgata gaatatactt acaaatccgg tattttgatc
     1921 agaaagcctt ttgcacgttt catcaaatgt cctttttcct tcctcgaggc ctccttcaac
     1981 taattccatg cgtgtttggc cattgagata acacatactt agcaaacctc cataccagca
     2041 cggctttctt gctatgttta ttcttcttac ggtacatatg tatgaatgta ccaaccattt
     2101 ccttaagacc gggaaaacct gcggagccca atctcttcca ggttgtccat acacttcagg
     2161 atttttggat attatagatg ctacatcctt aaagccattg ccttcgttcc tttcaaactc
     2221 atatgctcct tctctgaagc ctaacggttc aagatactca gctattgtag gatgcacgct
     2281 gtgcatattt gtatgataac caatctcatg ttccttaatt ttttccaata tatcgtaacg
     2341 gccacgttct ttcaatacac gagctttttc ccccacaatt ttaagaattg ctttcactcc
     2401 ttttgatttc aatgtgtcaa gcaaaagcaa taatgcatca tcagactgca aatttatata
     2461 atcttccaca tctaaccata aaaggacttt aatattcata ataatacctc atttctttaa
     2521 agccttatta tttaaagctt ttcctcttct ttaagaatct tcagtgcatt ttccctggat
     2581 actttttcat atgtcttgtt tgtaattttg ccattctgaa ctgcctcatc aagccagcct
     2641 gatgtgttaa gctgataatt tttatcgcaa atatcagtac caaaaagtat acggtcctgg
     2701 aattcttcaa gaaaatcata agaaaattct ggatcacgaa gcattgcatt attaccactt
     2761 cccgcagaaa tttctccata taggttggga tattttctca tcaatttgac aatgctgccg
     2821 tcagatatca ctttggtttt tggataatac caatcttccc agttttcttc tgtcagatca
     2881 tcactaatat gagaccaaaa agatggtgag tggcctataa aaatcaaatt gggaaatttg
     2941 gctagttcct tttcaagcct tggaagtcct acttcatcaa caagaccata tgaacctcct
     3001 attttagttg ccatatgaaa cagcaccggc atttgatttt tctggcagtg gtaaaaaaga
     3061 ttttctacaa aaggatcatc aaagtacata tttgcttcga tttcaccaac tcccttagca
     3121 cctctttctt tgtaatactc caagtaaaat gacaggtctg tcttcgtgct gttttgcccc
     3181 atacgtggat ctatattaca aaaccagtaa aataaatctg gatattgctc tacaatttcc
     3241 atagcatctt cattcccctg aatcattcct gcacattcag gatttacaag aggaagcagc
     3301 actccttttt ctactccgat tctttcatat atttctgcta cttcataagg tgtagcaaaa
     3361 gttttgtttg atttataaaa attaatccct tttattcgga ctgaatgagc atgaatatct
     3421 attttcttta tcatagtaat tatctccatt tatattcatt cttctttcaa aagttttaaa
     3481 gcattccctc ttgatatttt ctcatatgct ttataactga ttttcccgtt ttccactgct
     3541 tcatcaagcc attctgatat ttttagattg aattctttac aactaatatc agttccatat
     3601 aatatttgat cctggaactc ctcaagaaaa tcatatgaaa actctggatc acgcagcatt
     3661 gcattgcttc cgcttcctgc ggaaatgtcc ccatataaat ttggatattt tctcataagt
     3721 ttgacaatgc ttccgtcagg tattacttta gtcttgggat aataccaatt tgtccagttc
     3781 tccgctgtta aatcattgct gatatgtgac cagaaaggtg gtgcatgacc tataaatact
     3841 aaatcaggga acttcgcaag ttctttttca agccggggta atcctacttc atcaacgaga
     3901 ccatatgact ctcctatttg agttgccata tgaaatagca cgggcatctg atttttctgg
     3961 cagtggtaaa agaaattgtc cataaacgga tcatcaaaat acatgttagc cgcaacttca
     4021 cctactccct ttgcacctct ttctttataa tattcgaggt aaaatgatag gtctgtcttc
     4081 gtactgtttt gccccatgcg gggatctata ttgcaaaacc agtaaaatga atctggataa
     4141 ctctttacaa gctcaagagc ttcttcattt ccctgcacac ggctacggca ttcgggattt
     4201 acaagtggaa gcaatacacc cttctcaaca ccaattcttt tatatatctc aactacttca
     4261 tcagctgtag ccaatgtaat ttttgacttg aaataattaa taccttttgt aagaactgcg
     4321 tgagcatgaa tatctatttt ttgtagatgg atctcgctta ttttattatc aatgtcaaga
     4381 gtattcttaa ttcgattgtt catgtttagc tcctttcatt gcatatgatt ttctatatac
     4441 ctatgattct acttatgatt caagaaattt tagtgcattc cctctggata ttttctcata
     4501 tgccttgtaa ctgattttcc cgttttccac tgcttcatca agccatgatg aggtattgag
     4561 ataaaaatct ttgtggctga tatcagtacc aaatagtatc tgatcctgaa attcttcaag
     4621 aaaatcataa gaaaattccg ggtcgcgaag caatgcatta ttaccgcttg ctgcagaaat
     4681 gtccccatat aaattgggat attttctcat aagtttaaca agactgccat cttgtattac
     4741 tttagttttt gggtaatacc agtctttcca gttttcagct gtcaagtcat cactaatatg
     4801 agaccaaaaa gatggtgaat gacctataag aatcaagtca ggaaatttgg ccagttcttt
     4861 ttcaagctta ggaagcccta cttcatccac aagaccatat gactctccta tttgagttga
     4921 catatgaaac aacaccggca tattattttt ttggcaatgg taaaagaggt tttctacaaa
     4981 aggatcatca aaatacatat tagttccaat ctcacctact ccttttgctc ccatatcttt
     5041 ataatactcc agataaaatg ataaatctga ttgcggtgta ttatctccat tccggggatc
     5101 gatattacaa aaccagtaga aataatcagg atatttattt acaatttcta tacatacctc
     5161 ttcattacct tgaaaaatgt tgccaaattc acaatttcta agaggcatta ttactccttt
     5221 acccactcct gttttcttat atatttctat tatttcttca ggtgttgcaa tggttttgtc
     5281 agacttgtaa aagttgattc cccttcttgg aactgcatgt acatgaatat ctattttttc
     5341 cacaataatt cctcctctat attgattatt tatgttgatt ttagtatatg gctctattat
     5401 tgatataaca agcttcatta ttttacattc tatatcttaa ttttgctttt aataatgagg
     5461 taaagaatgt attcataagt aaataactat tacaatattt catattgcag tatatagctg
     5521 caaataaata aaaatgcgtg tgataaatta aggtgctgta ttttttcaag aggttgctat
     5581 ttttgtttat tttgatacaa ataatatata attaccacgc atttctcttc ttccctctgt
     5641 attctcccgg attaaccgtt gtaaattttt tgaagcttct cacgaagctg attctgttat
     5701 taaatccaac ctgctccgca atctcttgag tgctgatgtt tgtagttgca agcaaaacct
     5761 ttgctttttc tattcttatc tgattaatat agtctataaa atttgcgccc attatttctt
     5821 taaaaagcct tgaaaaatac gaagagttat aaccaaaatg gtctgatact gaagataatg
     5881 taatatcaga catatagctt ttttctatat actgtcttat ttttgcagaa aaatcaattt
     5941 cactttcatt cctaatataa atatgctgtt caacaacctt tttaaatact atacttaacc
     6001 atttcataaa ctgctcaatg tttttaaaat cgttaattga tatattctta cctgtttcct
     6061 ttggcataat gttttttgga tttatattaa actcaaacat gcatctgtta actagattaa
     6121 taagattaga aaagcagata aaaatgtggt caatattttt atttgcatca atgatgtttt
     6181 taatgccatt attaatgcat aaaataacgt tttcataatc acacaggtca ataaaagaaa
     6241 tgatgttctt ctcaatttca attggataaa acagtttact gtcttcagaa taattgatat
     6301 tataggcatc tactatatca cccataataa aactatactt cagagccttt aaagattctt
     6361 tatatgaaag ttcaatatcc aggacatctt cgtgcatctt tccgatacct agaataactt
     6421 gggaacttag ataatgttct atattatttt ttattgtttg catgcatttg gaaattaaag
     6481 aatccagctg ttctttaact gtctcagtat atatcttaat aagaataaga atattatcat
     6541 cagtgtcctg ggagcaaatt acatcaattc cattattctt aaaagctgct tgtacaatat
     6601 taattattgc catcttctta acttcaaact cttcaatatc taaatcaccg gggtaaatac
     6661 tgatattctt tatcgtagcg acacgataat atttacagtt tatatgaatg ttaaaatatt
     6721 cggctttctt tgtaataaat tctcttgtcc taatttttcc ggttatcaga ttacttataa
     6781 aatattccct gaacaacagt ctattttcat caagaacttt ttccaggtct tttctatctt
     6841 gaagcaacgt cttcaatccg ttctttatta taaagatatc actgttataa tatttatttc
     6901 ttgtatcaca aatttcatcg gcttcattaa tttcttcagc atcattgcta gtattgttat
     6961 tagtattgtt attagtggaa tcatcatttt tatgattagt ttctgatttt aaaagtaaag
     7021 taatttcttt aatcggtttg taaatttttt tagatagcag taatgatact attattataa
     7081 taattaaaat ggtaattagt accaaaacaa ttattaaacg tatttcaagg gattttttga
     7141 atattatatt tgaaggtatc attatgcaaa gtttccaacc atttatatct acatcggaat
     7201 aataacagac catcttacag tcggccagat ttatatcggt tttccctaat ttttcatctt
     7261 tgaaatggat ctgtccaaaa gtaatctcat taatatcttt atataaatat tcatcatgat
     7321 ttgatgataa tactatacca gcttcattta tcaaaaaagc aagagattgg ctattatttg
     7381 taatgttttt caatatatct gatacaattt cataattcaa attaataact attgtaccta
     7441 gctttttttc attatcatat ggaatcccga taaaaaatgt tatgacattg ttattgtaat
     7501 ctgtataatt gcctcttcga aattttttac tattatccac agatctggta tcaattattt
     7561 tacaattacc cgtcatttcc tcataatatt tttcccaacc tgtttcataa aaaaaatggg
     7621 gtgcatatat ccccgtataa ttaagtacca cttcgctttt cccataatat acaaaaatat
     7681 cattaatata gttgcttgaa tttattacac cggataaaat atccataagc ttctttatag
     7741 tgcgataatc aagaataact tcacttcttg taagtgatac aaactggtta aagtaaggat
     7801 gtgaggcata atacagaccc atattttcaa attccttaaa cctcatttta atactatttg
     7861 cagttttttc aacctgacct ttctcaaatt tctctatttc cctgtcaaat tgctgcatta
     7921 catagttatg agtaaatata ccaactatga tagtcaacag caaagatata gatatacata
     7981 ctataaatat tttcaaggaa agatttatac tacgcttgtt aaaaatgttg ttaattccat
     8041 gttttatatc catcctatac cactccaatt tgataatata ttgtaatgat attatgttga
     8101 atatataaga agaaagccca tgtggtattt tttgtcataa tcaaagtaca cctgactttc
     8161 aattttctta actcacaatg cttgatcatg attttagtat ataactcaat taacagacta
     8221 acaagcttca tatttttact ttttgtatcc atttttgtgg tatacaaaat aaggtaaaaa
     8281 gtatatacat atgtaaaatt tatttacaag ataaatacac ttccttcttt aaactttcaa
     8341 agtgttaatc taatatagct gcctttttta ttgcaaatca atatccggtt ttttataaag
     8401 atttcgtata gtccagaata acagtcccat cttccttata aataactgca tttttatctt
     8461 gagtcaatgc atctatatct ttaaaatatt cagtttttga tgcatacctc aaatcttttt
     8521 cagatattac tctttttgct tctgcagcat cattagacaa aagtgtatgc agcattacaa
     8581 gctggcattt tgcggaattt atacaggcag tctcatgatc ttctatatgg taattactgc
     8641 catgagagtt gcctgctgca cctcctacat atggatgtat agcaggcata acagcagata
     8701 tatctcccat atcggtacaa ccggtattcc agctatcact tatagcaata tttccttcgg
     8761 gtacaataat cttcatggct ttctccgcaa tatgtaatag atttttgtca ttaattaatg
     8821 gagtgtagcc aggacgatca ctgagaaaca caccggcacc aattgatgct gcagcgccag
     8881 caagggcacg gttaactttt ttattaattt cactgacgac ctttatatca gcaccacgaa
     8941 catagctctc cattgcaacg tcattgggaa ttgcattgac agcactgccc ccccttgtaa
     9001 taatagggtg aactcttatg tgatctgagt ctctgaatgt ctcacgaagt gcgttaattg
     9061 cctgtatgcc caagtttgca gcatagagag cattcacacc ctcatggggt gaacctcctg
     9121 catgggaagc aacaccttta tagtttatgt tcttaacaat acatccatta gaacctctat
     9181 atgcaaaaaa atgatccttt ccaactttgg tatgaatcat gaaagccata tctacatcat
     9241 caaaataacc acgatataag aattccactt taccgccaaa ataacggata atacctgctt
     9301 ctcgcaaagt ttcccggtaa ccggtctcaa ttagttcttc ggccggtact gcacagaggc
     9361 gaatagatcc tgatagtcca tctaatgcgc catcttcttt taatgaagca gctaatccaa
     9421 tcagagctgc acactgtgca tgatgcccac aggcatgtac tgcaccggtt ttaggatctg
     9481 catcaggatg atctgcacat ataagtgaat ccaactctcc cataatgagc acacaaggac
     9541 ctggacgccc tgtgtccaga gttgtataaa agcccggaat attacctgct ttcacgagat
     9601 tataacccag cctctcaaat tctgcttcca gataagcact ggtcttccat tcccggtagc
     9661 cggtctcggg atgctcccag atatactgtt ctgctgcaaa aattagatct ctgtacatat
     9721 caacactttc caaaagctct ttatagtctg acatatagtc tgacataatc atagtttttg
     9781 ctcctctttg tttacagtaa ttatttacag tgaattaagc aatatttcac catttctgta
     9841 tgcaaccttt tcaatttcag atacatcaag atcactatag ttcagtttga acacatttcc
     9901 ttcaggataa tagaaagcgc tatgtgatga aaacaaaatt ttgtcaagag agtaagcatc
     9961 acggattgta tgaagtacat tcgggcattc aacacttgaa atatcaatat atacattact
    10021 cgtcgaatga agagcaggag caacttcacc ccaataaaca ttattgagta taaatgcttg
    10081 ctgagggaaa ttcgatagtg ctcttatagt tgatagcata tcaacatcag gtatcttcat
    10141 tagaggatat tgccctcttg tatcctcaat tctcatttga attgctatga tgagcttatt
    10201 atacattgta agatttacaa gcttttcaag tttttcttcg cttagctcat atatgtggta
    10261 atttggtatg agttttatca ttttaactct tttatcctgt attgccagtt cgactacctt
    10321 ttcccagttg gcataggaaa gatcgacaac agggacaggg gagaaaaagt catccttgat
    10381 atcttgaaga agctgtttat ccgcacgcat tggatctcgt tcaagaatac tttcaatagg
    10441 ggatacaaat gcctttatca cattttgtga tttcaacttt tttttcaggt catcaagtcc
    10501 cgaattcttc aggtggataa atggccaatt tccggtccag gttgaaaaat caataatttt
    10561 atttatatta tacatgcctg ttcacccttt caccctttct ataaaattct aatgcattta
    10621 aggataagat tttttctcta tcctcatcac tcatatcaac tgagaatatc cgtccaagct
    10681 gtgtagcaat atctcttcca ccatagtcag aaccaaaaag taccctgttg ggtcctaatt
    10741 ctgcaatggc atattccaga tatccatctt ccggctgccc tccgctggta tctatccaga
    10801 cattcgggca atccttgata tcctgaacac ctctaagtct acagcctgta aggtgagcca
    10861 taaggatcct caatttaggg aattttcgtg aaagaacagc aatgtccgaa ggatcagatt
    10921 ctgctccata tttactaact gttttgtacc agctgtggaa aagaacaggt atatcatgtt
    10981 tttcaaggta ttctgccaga ggttcaagtc ttttattgcg tgcattaatt tcaatccaaa
    11041 gcttaacacc tataatattg ccattgatta tgtacctgtc aattaactct tttgtaaagt
    11101 caaatggaag tgttgggtta atccatagta atggatatag tatgtcttta tgcttttgac
    11161 ctatatcgtc tagaattgct gcctgaccgg tcatgtcatc cttgctgaaa tagagtgaag
    11221 atacaggctc cggcattacg caggaacgtt ccactccaac aagcttcatg ctgtccagaa
    11281 gcttttgaac tgcaccttca gtgttttttt caccgactaa agtcacatgc agatgactgt
    11341 caaatatcat caaaataccc cctctaataa ggctgaatta gtaaaatact catatacaac
    11401 aaaataatta tatatcagtt attacattaa tgcaaaaatg attctgcacg gagggtgcat
    11461 atttcaaaag tgaagaaata tttaccagac caatattttc ttcacttaaa ttatatgcac
    11521 ctatttagca atatcccttg aaatgtgaaa aataggagag taatataaat atcaacgaat
    11581 ttaacagcaa ttatattagg agaaaaatac tatgcaggca atttttgaaa ctctttttga
    11641 taccatatac cttataggtg ttattacagt tggtataata atgatgaaaa atgcaaaaag
    11701 caacaagcaa acttttttgt ttggttttat gtcagtgctt ttaggcttag gcgattcatt
    11761 tcatctggta cctcgtgtat atgcattgtg gacaacagga cttgaggcta atgctgccgc
    11821 tttaggtact ggcaagctta ttacctcaat aacaatgact attttctatg tcattttata
    11881 tcatatttgg cgtgaacgtt ataaaataag taatagaagc agcatcattt attcagtcta
    11941 tggaatggca atttgcggtt tgttattata tatcaaacgg taaaattgtt tgatcaaaac
    12001 aaaaggggta gtgtaaatgt cactcccagc aagattttaa atacttcctt cactgataat
    12061 attgtacatc ttgttgttta agtattatta atgtttgagt atttattttt tgattttttt
    12121 atgttttgat aagtcttcat aatttatttt aaatacagca tagtctccca taaaatgagg
    12181 aactcctata gaaataacca atgagtccct ggttaaacat gaataaaccc cgctataatt
    12241 atcaacacct gcggtatcag cattgtttaa tctatttatc cagccatttg tatcagtctg
    12301 gttaagttct tccgtaagtg ctgcctttaa ttcagatgtt atttcttcat cgcccattga
    12361 gatatatttg ccatttttaa agatattaac aaagtcctca tcaatattta caaagtcttt
    12421 taattttaaa acttcctgct tttcaatatc gatattagta gtataaataa agttatttgg
    12481 atgtgcagct cccttaaaaa aactatatcc ttcatattta atacttagca gattttcgct
    12541 ggtatacatt acatcatatg ttaattcata tgtcacctct tcagtcactt ttgcaagttc
    12601 atctttgtcc tttagtgcat ttcccttaat aatttcattt atattatttt gaactgcact
    12661 atcagtcata ttcaccactt gtggataatt tatagctatt ttgccgtaag taaaggtttc
    12721 agtttttaat tcatatttta tttgtttgtc tgcatgtttt atttctgtag atatatcttt
    12781 catttctgta gctgcatctt ttatttttgt ggctgctttt tcttcttctc tttccttacc
    12841 tgcttctgtt atttttgctt tttcagttga taaaacagct ggttgtctgt ttctgccgca
    12901 tcctataaaa cacactccta atagtgttat aagaataaaa gccagtatta atatattttt
    12961 cttttttatc attcctgctt ttacctccca actttctaaa tatagattgt ttaataaact
    13021 tttctgtata tctgcactcc ttttgtatag cagacatcgg cttttctagt gaatgaccac
    13081 aggtcttcaa aataaggctc gcatgtacgg atactgtata acgataacgt tttagtctgg
    13141 tatactgtat ctggcaacct tttacattat cccaggtaag ttttgcctgg gaccatgaag
    13201 aaccctcgtc ggacataatc cataatttcg ttgtaagata tgtaaataat tcatatatat
    13261 ggttctgtgt tattgggttt tcttcatccc tgcaaattga agatttcctt tcgtatcaag
    13321 ggtgaatgta taacattcat caatcacttt atggaaaaag gtatcctttc cacagggata
    13381 cactgacaaa cgcttatagc aaagccagga tccattgcaa aaggaaaccc ttcacaataa
    13441 ctgtcaaccc atttatctag ttgttctgtc atcattttga tatctcctat ttgattttat
    13501 ggcatctaca acaatacatc tgtatttatg gtattattat caacatcatt agcaatatta
    13561 ttatcaacat tatcagcatt attatcaata tcaatttcaa catcaataaa atcatgtgca
    13621 gcatcacgca taagcttttt cttgacatat gcatagcata ttaaatgtat agagaaggta
    13681 acaatccatg aagcaggata gcacagataa agtacctcta gcgttctatt cattgcaaaa
    13741 atagtatata tccaggctat tctaattccg catacaccag ctactgacac aagcatcggt
    13801 gtcagtgaat atcccattcc tcttaaagaa cctacaataa cttccataat accgcatgta
    13861 aagtatgtca atcccataac acgtagtctt gtcataccat attttaccac atccatatta
    13921 tccggtgaat agatacttat caaagtttct ccgaaaatgt atagaagcgt accgagggac
    13981 aaacctataa ttgttacaaa aaagatacat atccttagaa ctttaccaat ccgctcatat
    14041 ttttttgcgc ctacattctg tcccgtaaaa gctaacgaag catgataaaa tgaattcgtt
    14101 gccatataaa tcaaaccgtc taaatttgaa ctggcagtat ttccggccat tgctaactgg
    14161 ccgaaagagt tcacagaaga ttgtaccaat acgttggata ttgaaaaaat ggtgccctgc
    14221 aaacctgctg gaataccaat tttcatcatc tttattaact tatctttatg aattttaata
    14281 tcacacagtc ttaaacggca agatccttca aatcttataa ggcacagcac aaccagaact
    14341 gcagaaataa tttgtgaaac taccgttgct atagctacac ctgctacacc catattaaac
    14401 tttattacaa atattagatt tagcatgaca tttataagcc ctgaaattgt caaaaacata
    14461 agtggccgct ttgtatctcc aactgctctc aaaaccgaac tgccaaaatt aaagaccatt
    14521 gttgccagca tacccataaa atatatcctc atatataata cagagtattt caatacttca
    14581 tcaggtgttc ccatagcttt aagaaacact tctgccatgc ttatcccgat tatgcttgtt
    14641 ataatgccaa aaataacact aatacctatg gctgtatgaa ctacctggct aacattctct
    14701 gaatcttttg ctccataata ttgggcaaca cacacgctta cgccaacaga caaacccatg
    14761 aaagcagtta taataaggtt tataagtgaa ccggtagaac ctaccgcagc tagtgctatg
    14821 ctccctgcat aacgcccaac gacgataaca tccgccgcat tatatgccaa ttgaagaact
    14881 cccgatatca ttagagggat agaaaaaata ataatctttt tgaacagtga gccactgctc
    14941 atatccattt cattcctatt agcactcaca ttaataccca catttattca ccaaaataaa
    15001 attattaaaa catattctaa taattttata tccatctatt tcatatcttt ctatttttat
    15061 taatgattta atacaacact tcacaagata tgagtttacc atatacaggc ataagaaaca
    15121 agacaaaaat tctggcagga catatatatg taataaaaaa agaattgtcc ctgataaatc
    15181 tctctatata tctttatatc tccctatatc tccctatatc cccctatatc tccctatatc
    15241 tccatgataa atccctaaat ccctggtaac attaacgtat gagagaaatc atcttgttca
    15301 acgatagttc atagaagcta tctataatta gatctgcttc agataaatct tgattcccag
    15361 agtttggact tctgtatgct atacatttta taccagctgc ttttgcagcc ctaactccgc
    15421 tatgggcatc ttcgataact atacagtttt caggggtaat ataagatgaa gcatcagtgg
    15481 aggtttcaat catgttattc aactgtgata cagccaattc aaatatatct ggagcaggtt
    15541 taccattttt tacatagtct ccgctcacaa taacattaaa gtattcccta aaaccggttt
    15601 tgttcaatac agtttcaata tctctaattg agcttgatga agctaatgca atttttatgc
    15661 tattgttttg taattcttcc aatagttctt taatacctgc aattgactta atcttctttt
    15721 gtgactccaa aaattcactt attcgttttg cttctatttc caccagtttt tctactgaaa
    15781 attctatatc atatctttgt tttaactcct gccacatatc cacagttctg atacctacat
    15841 acatataccg atgctcatca gtaaggtcta gccctaattc cttgaacata ctcatttcaa
    15901 ctttatggta tataggctca ctgtctatta taatgccatc catatcaaaa attactgctt
    15961 tcagcataaa ttaaaatctt tcctttctga ctgtcactat caattaattg cataccatac
    16021 ctaatgaact acaaaatata ttttaatata tcttattgtt ttaaaaatct catctcaatc
    16081 tcaatctcaa tctcgcctca ttattctaat tttaacgatg aatgatgact gcaccatcac
    16141 tcaagcaatc aagttttatt tccatggagt atttgagaat ctgcgtatat tgatttcatc
    16201 tatcactgca tttcccctat gagtcaaaag aaacattaca acatccgcta tttcctgcgg
    16261 tgttgccagc atggattcat caaggtcggg tcttgttttt gttaccatat cagtagcaac
    16321 ccccccagga gcaatgatgt gaactctcac attatccttg aagacctcct gtgccaaaac
    16381 tttggtcatt cccattagag catgctttga tgccgtatat gcaccttggt taatataccc
    16441 cttataccct actacagaag atatattgat aattgttgcc atatctgatt ttttaagata
    16501 aggcagcgct tcacgactta ataagaaagg tgctcttgca ttgattgcca tatgcatgtc
    16561 ccattcattt accgttgttt cactcaagga tttaggcaca gctatccctg cgttattgat
    16621 aagaatatct attccaccaa agtgttgtac aaccttctca attattttta cacatgcttc
    16681 aggatcgcta agatcagcag gacatgataa tacatctatg tttaaacctt taaattcctc
    16741 tttcattgcc tcaagttggt ccaaagacct gctgtttaat gccagattta ctccaaactc
    16801 agcaaggctt ctggaaatag ctttccctat tcccctgctt gctccggtta caattgctac
    16861 ttttcctttt agtgtcaaca tattttccct ccttaattct actatttgca tgaacatgaa
    16921 gaaatatccg atatcctatt attcaaccta atgcatttaa aacattgtat cattattgca
    16981 ttaataattt caagaattta aaggggacat tcctgcacac taaatactct tattaacatt
    17041 tgtcacattt ataacaatat atagcaatat atgttaatag ctataaaaaa tcttaagtag
    17101 tagaagagaa aatctataga gttcgtgtat catgtaatgc tgaggagcat tattgaaata
    17161 aacctgtatc aatctagtga agacaaaggc cactatctta aacttaaaga cttaaagagc
    17221 tcataatgga ataaaaaaag gaatgtccct gtaatttcac agaagaaatg tccctataat
    17281 ttaagtttcg gcggaagagt cttttgctct cccgcccacg caatctgtaa cgttccagct
    17341 cctgaaatgc gtgatttcag tagcaagttt tcaacatgct tccaagttgc attcgtcttt
    17401 tgggtaaaac ttgctttttg tgtaacaatc atattactga ttctgctgat tctgcgttat
    17461 aacttttata ttgtccactg attgtgaata attcctacca gcttccagcc atcctcatat
    17521 tgttcaaaga ctaatctaag gcttttccaa tccatgcctg catattcagg attaaaaccc
    17581 ggaaaataat attctactac gatagcattt ttataaactt caaattgatt ttcaatcata
    17641 ttcccgttgc ttaatacttc attatatcca atctttttag catttacaaa atcttcagag
    17701 tatatgaatt tgtcgtaata ctcacccgga gttaatctga tttcctctcc gcttccgtca
    17761 aaatgtcccc ataaataaag gttcctatca tcaaaaaagt ttttcatttg ctgcttgtta
    17821 aagacagcat cgtcttccaa tgaaacactg gtataagggg taaatcttat acccttatcc
    17881 gggtgtacat attccgaaat tgtttgcgca tctttattac tcatagcact aattaattta
    17941 tctgctgttt cttttataat ttgctcggcc gcatctggtt ttttgagttc ttcaccgaga
    18001 ctaaattcta ttacagttgt tagct
//
LOCUS       DAOSDD010000068        18007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1267_length_18007_cov_4.895276, whole genome shotgun sequence.
ACCESSION   DAOSDD010000068 DAOSDD010000000
VERSION     DAOSDD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18007
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_18007_cov_4.895276"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="ilvB"
                     /locus_tag="PK733_07940"
     CDS             complement(<1..1373)
                     /gene="ilvB"
                     /locus_tag="PK733_07940"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOJ10508.1"
                     /translation="MKLTGAEIIIECLREQGVDTVFGFPGGAVLNIYDALYKAQDKIN
                     HILTSHEQGAAHAADGYARATGKVGVCIATSGPGATNLVTGIATAYMDSVPMVAFTGQ
                     VATSLLGKDSFQEVDITGITMPITKHNYIVKDVNKLAGIIREAFYIAGKGRPGPVLID
                     VCKDVTAAVTEYEPEAPKLPDNISIGLKNNDIEIAAQAINRSHKPIILSGGGVSIAGA
                     DKEVRILAEKARIPVTTTLMGLGSFPATHELYMGMVGMHGARTTNTAIYESDLFIAIG
                     ARFSDRVISDVKRFAPEAEIMHIDIDPAEIGKNINVQYPLVGDIQEILRLLADKVNGR
                     EDNEWNSKIKKWKSDFPVKYIQDGSLKPQYVVERFYELTNGDAIITTEVGQNQLWAAQ
                     FYKYTKPRQFISSGGLGTMGYGLGACIGAQIGKPDKKVINIAGDGSFRMNCTELATAV
                     EYKLPIIV"
     gene            complement(1466..3124)
                     /gene="ilvD"
                     /locus_tag="PK733_07945"
     CDS             complement(1466..3124)
                     /gene="ilvD"
                     /locus_tag="PK733_07945"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOJ10509.1"
                     /translation="MKSDIIKSGIEKAPHRSLFKAMGYTDEEINRPLIGIANSKSEII
                     PGHIHLDKITEAVKAGIRMAGGTPVEFGVIGVCDGIAMGHIGMKYSLATRELIADSCE
                     AMALAHSFDGMVFIPNCDKIVPGMLMAAARIDIPSIFISGGPMLSIKHNGEYIDLNSV
                     FEAVGAYKAGKMTEDEVRDCEENACPGCGSCSGMFTANSMNCLTEALGLGLPGNGTIP
                     AVYAGRIRLAKLTGMKILELVEKNIKPSDILVPEAFENALTVDMALGCSTNSVLHLLA
                     IAHEAGVNINLDIINEISSRVPNICKLSPAGKHHVQDLHAAGGVQAVIKELTGRNLIN
                     KKLMTVTGSTIEDNIEKSRVLDREVIRNLDNPYGITGGIAVLKGNLAPEGAVVKRSAV
                     APEMLCHIGPAKVFNSEEEAIDAIYNGKIYKGDVLIIRYEGPKGGPGMREMLSPTSAI
                     AGMGLDKDVALITDGRFSGATRGASIGHVSPEAMEGGPISVVCDGDIISIDIPGGKIN
                     IEITDEELERRMSMWKAPTPKITKGYLGRYSKIVSSASRGAVVS"
     gene            complement(3415..4125)
                     /locus_tag="PK733_07950"
     CDS             complement(3415..4125)
                     /locus_tag="PK733_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOJ10510.1"
                     /translation="MNWSDLAVAAIIIGFGLIGLNAGFMLSVFKIASYFLSIWLSFKL
                     YPKVSQLLMNTAIFENIKNAIFDNLLKQKGALINGAGEQVKQAAADMVINKLPLPEFL
                     KNNLTSQIPDPAKLIDITKVMESISGELAKIIIQILSMLLLYISIRIILIICKYLLEG
                     ISKLPLFKQIDKLGGFALGAMEGLLTVYIVCAVLMLFNTTPSFRPVYEAIEKSMFAKF
                     FFEKNFIIDFMFPGKMKI"
     gene            complement(4240..5403)
                     /locus_tag="PK733_07955"
     CDS             complement(4240..5403)
                     /locus_tag="PK733_07955"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOJ10511.1"
                     /translation="MGTNEKKSGVFLFILLLIAIMFTAYIAYLKSNNIDISKIGLKKL
                     VSAAIQGHEIDADNANIFEIGYEAKDKPSFEVYREWLIKCTLYSVTAIDKKGEEQWSI
                     PILLNKPMLQSQGGRLLVSDIGGRSIYVIEGKNIKWQKAVDGNIINASINETGYVSVV
                     HETEGYKGMVSVYDVDGQEIFHRYINETFVLSSQVMASGQQIIINNIDASGSTAYTCI
                     DFTDLMGNPFAALVPEQGAIYPFILALSDDSFALANDVSIIYYNKNREKLWENKYKKI
                     YGCKEISDKSFIAAVDDINDTGGRQKFSGVIILNKDGRITARCPIDGQVCNISTFKDI
                     AAVNNKREVYFINSKGTLEGKYSSITDIEKVIFFNKNEAALITKSRIIIINIK"
     gene            complement(5585..6979)
                     /locus_tag="PK733_07960"
     CDS             complement(5585..6979)
                     /locus_tag="PK733_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOJ10512.1"
                     /translation="MKLYRNAIILVIILALLFGVYAFAKNQKSKTEVTERKSFEKIID
                     LDTTEMEELTVQKGSDKLTIAKKDKEWELKYPEGIRYDSSKLSSIAINFSSVFIEKEV
                     EENATDFEQYGLDKPIIVSAKMKDGKVETLEIGNLTPTGGAYYVKKKDSSKVYTTDTY
                     TINKVIVGKNDIRDVALFTIESTDITKISMERKGSVVFEANINSEENNWTLTSPIKGN
                     ANISALSPMLDAITQSQITEFVEENPSDLEKYGLAKPSYVLEFESSQGENRLLLGKEI
                     SENAKVYAMLDGVNEVYTISSEPFNFLDKPIEEIVEVFAYIVNIWDVEKINVEMDGYN
                     LNLELQTDPDQDTDKDKFFVNGKDASMKDENDRQPFRSYYQSLIGVTLSQVQPDGQPS
                     VKPDITFTYYLKKDPKVMKVEFIPKDDRYYYVVKNGEYSGILVDKKKFDEPEGVRESY
                     AKLMETLKKAETAQ"
     gene            complement(6976..8634)
                     /locus_tag="PK733_07965"
     CDS             complement(6976..8634)
                     /locus_tag="PK733_07965"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOJ10513.1"
                     /translation="MNKNEEMNKINNNAENEVTVTADINEETDNISKACNNEENNNDN
                     NENDNNIKNAEETNNIKNSNSKNKGTNNVKKTFNKVVKNRSFRYGTNSIILIAAVVAI
                     AVVLNILVGLGQVKWDLTPNKLFSIGDTTKTILKELDQEVVIYGLFDDGKIGPSSEYK
                     EVVELLDHYAKYPNITVKYVDPDKNPGILKQIDADGVLKDISKNAFVVKSGRRIKGLS
                     YYDLFSADFNQQTFQYYKTGSTAEQGFTGAIKYVTAEFTPTIYFTTSHDEKDIEKNFS
                     TLKAQMEYNNYDVKPINLITEANVPEDTEILVIASPKKDLTASEKDKIKEYLKNGGKA
                     IFMFDYLELDPQFPQFENILMDYNLSLNYDKVKENDQNRHLPQNPYVVLMDVKSGAVI
                     SDNYNILLANSRSINILKNTKEYINVIPLARTSSKAVGEQVDKSKGNDIEGPLDVAVA
                     VEHKGWQKISKIAVIGNSIFLEDSAQDAFGPYFSNGMAFFLETMIWMQDKKDETIIKP
                     KNYQQQYLNITAEQAQYMGIIVVIVVPMLILLAGVFVYLRRRHL"
     gene            complement(8640..9344)
                     /locus_tag="PK733_07970"
     CDS             complement(8640..9344)
                     /locus_tag="PK733_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOJ10514.1"
                     /translation="MRAVFWKEVKSYFYSPIAYVLIGMFTLISSIMFWPNLLSGYGEF
                     NGNLGSMGYILIFLVPILTMRIIAEDRKNGTEVLLLTSPINLGGVVLGKYFAALSVFL
                     MMILVSFIYPIILVIFGASITPQIVGGYAGLILLGASFISVGIFASSLTENQVIASII
                     SFVALLIIWIADGIGGMVGGFTAKILYWFSLLSRYEDFNRGILSLSAVVYYLSFISVF
                     LFVTIMIIERRRWSQR"
     gene            complement(9346..10293)
                     /locus_tag="PK733_07975"
     CDS             complement(9346..10293)
                     /locus_tag="PK733_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10515.1"
                     /translation="MIEIQNLTKYYGQIVGVKDLNFTVEKGEILGFLGPNGAGKSTTM
                     NIITGYMPSTQGTVKVCGYDILEQPIEAKKRIGYLPEQPPVYMDMTVLDYLNFVSELK
                     KVDKKIRKGQLDDIMEIVKITDVRKRLIKNLSKGYKQRVGLAQALIGKPEVLVLDEPT
                     VGLDPKQIIEIRKLIKALGKEHTIILSSHILTEVSAVCERVVIIHKGEIAAIDTPENL
                     SKNFGNMSKMSVTISGPKNSVVNSIKGIYGVKYIEPSAEKEKDVITYIIESDKDIDVR
                     KPIFFAMAKAGYPIMELKSLGMTLEEIFLQVVTEEKEVG"
     gene            complement(10520..13318)
                     /locus_tag="PK733_07980"
     CDS             complement(10520..13318)
                     /locus_tag="PK733_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOJ10516.1"
                     /translation="MSEDFPSVKEGRNKSLGLSEREAREKFNKYGPNSLAGKAGISPF
                     KILVEQFSDFMVLILLAATAISAFMGETMEAMTILAIVIVNAFLGFIQEYRTERTLEA
                     LKSLAAPSAKVIRDGKPVTVSADKVVPGDLILVEAGDRVPADAILIESANLNVDESLL
                     TGESVPVEKHHRRNNGKKIIKEQNIDIYHGREFKREHEDYINSELDFSDKSRHIYMGT
                     TLTMGRGKAMVYATGMNTEMGKIAHMIQNIDDEETPLQKKLAHLGKFIVFACLVICAV
                     VSVTGIMRGEEVFNMLLAGISLAVAAVPEGLPAIVTISLALGVQRMLTRNALIRKLPA
                     VETLGCANVICSDKTGTLTENKMTVRKIYAGENIYDITGIGYDIEGDFILKGRKIEPL
                     KSNHLKLALEIGALCNNSQLIKKRHNKESLNNKIKSVFVHTEKWTINGDPTEGALLVA
                     AAKSGITQESLEKDYYRYHEIPFDSERKCMSVVCKNLKGEEFIFTKGAPDIIIKKCSK
                     MLTSKGIVNLTNIMKMGILKVNDNMAVEALRVLGVAYRKIDISNMNTSMDPSKSNLES
                     ELIFVGLLGMIDPPRQEAALAVQKCKMAGIKPVMITGDHKITACAIAKELDIFSEEDK
                     VLTGNDIETMGEDELLRQVENVSVFARVSPKNKLSIVKAFRKLRHIVAMTGDGVNDAP
                     AVKEADIGVSMGIMGTDVTKEASSMILMDDNFATIVAAVEEGRVIYSNIRKFIRYLLS
                     CNIGEVLTMFLGMLLGLPVPLLPIQILWVNLVTDGLPAIALSFEPAEKDIMMRPPRDS
                     NDNIFSDGLLSLIIVRGTLLGISTLAVFTAILHFTHSIDLARTGGFVTLVITQLIHVF
                     ECKSERRSIFEIQVFSNIYLVLAVLCSLFMILSVVYVPMLQGIFKTVPLTHHEWLFVI
                     GFSAIGPVGSSFFTKKRC"
     assembly_gap    13633..13642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13643..14183)
                     /locus_tag="PK733_07985"
     CDS             complement(<13643..14183)
                     /locus_tag="PK733_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOJ10517.1"
                     /translation="MPYFLEILARLVLALLLGGAIGYEREHENRPAGFRTHILVCIGS
                     ALVMTTSEYIFKLFSPIVNLDPARLGAQVISGIGFLGAGTIVREGFNVKGLTTAASLW
                     AVSCVGLAAGIGFYEGAITATVLIYITLIMLKKIESQFNVRSQYKNIFVETAAIPEII
                     YKLTDIFCRYEVMIKNIEYF"
     gene            complement(14245..16062)
                     /locus_tag="PK733_07990"
     CDS             complement(14245..16062)
                     /locus_tag="PK733_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOJ10518.1"
                     /translation="MKKAFLRTILVTIVLAVIGAFGYSAYYFFFMPNNSITAAFETEG
                     LHLVIEDEEVVSKYPPKIMDDEILLPLEIVKKYFDKTIYWDESLKKVTITTKDRVIRM
                     KTNELEALVNNKPVNLNIPAIEENGEVYIPIEFLSDFYNIDIDYIKSDNVIIIDPRYS
                     MVQIAEPISPKAVIRKGPSKNHPIIKKLQFTEINNETNSYKVDDDNGNSGKTLLRIFK
                     EYDKWYKVRTQEGEIGYIEKKFVSVKWLTVNLNILPREKEMELWNPEAGKINLVWEMM
                     YGQRPDLSKIGKIEGLDVISPTWFQLKDDEGNVINRADATYVDWAHKNGYKIWALFSN
                     DFQDIKASSRFLNNTDARDNAIRQILTYASLYKLDGINIDFENIYKEDKDALTQFVRE
                     ITPFLKEQGLVVSMDVTIPDGSDTWSKCYDRTSIGEIVDFVMLMAYDQHWSSSPVAGS
                     VAQLSWVENNIVKMLKIVPKEKLLLGLPFYTRLWQEETVKGGRVKVTNPKVLSMDNAR
                     KMIAENDTTVKWDEESGQFYSQFNKDGKVYKIWLEDENSINLKSSLVHKYKLAGTAAW
                     RRSDEAAVIWNILNENLKIVNNYQEWQDRNKITNYVYNR"
     gene            complement(16241..16744)
                     /locus_tag="PK733_07995"
     CDS             complement(16241..16744)
                     /locus_tag="PK733_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HOJ10519.1"
                     /translation="MDSFIEKIVVKKRTAKDSLIITGVVLGGLVLFFVLQLIPFLRPF
                     FIVIVAALVYFIYQFVISRNIEFEYIVTNGDLDIDKIIAQRRRKRIFSANCKDFDMVS
                     RLKGGNNDRRIQDIKNRIEAVSSMDSENVFFATLLYKGEKTAVFFEPDEKMLNSFKTF
                     IPRKMEI"
     gene            complement(17029..>18007)
                     /gene="groEL"
                     /locus_tag="PK733_08000"
     CDS             complement(17029..>18007)
                     /gene="groEL"
                     /locus_tag="PK733_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ10520.1"
                     /translation="ILITDKKISNIQDILPVLEKIVQQGKKLVIIAEDVEGEALATLV
                     VNKLRGTFTCVAVKAPGYGDRRKDMLRDIAILTGGEVISEELGLELKDTELSQLGRAR
                     QVKVEKENTIIVDGYGKPEEIKKRIAAIKAQIEETTSDFDREKLQERLAKLSGGVAVI
                     QVGAATETEMKEKKLRIEDALAATKAAVEEGIVPGGGVAYINVIPKVKELLEKVAGDE
                     KTGVQIIVRALEEPIRQIAGNAGLEGSIVVEKVKNSEPEIGFDALNEKYVNMVEAGIV
                     DPAKVTRTALQNAASVAAMVLTTESLVADIPEPEPPMPAGGGMPGGGMY"
BASE COUNT         5238 a         3869 c         2595 g         6295 t
ORIGIN      
        1 actataatag ggagtttata ttcaactgct gtagccagtt cagtacaatt catcctgaag
       61 ctcccatcac cggcaatatt gattaccttt ttatcaggtt tccctatttg tgcacctata
      121 catgctccaa ggccatatcc cattgtcccc aggccacctg aggaaataaa ctgccttggc
      181 tttgtgtatt tatagaattg ggcagcccac aactgattct gaccaacttc agtggtaata
      241 atagcatcac catttgttaa ttcataaaac ctttcaacaa catactgagg cttcaaagaa
      301 ccatcctgaa tatactttac tggaaaatca gatttccact tttttatttt gctattccac
      361 tcattgtctt ctctaccgtt tactttatca gctaaaagcc ttaatatttc ttgaatatct
      421 ccaactaaag gatactgtac attgatattt ttacctattt ctgctggatc tatatctata
      481 tgcataattt cagcttcagg tgcaaatctt tttacatcgc ttataaccct gtcactaaat
      541 ctggctccta tggcaatgaa taaatccgat tcatatatgg cagtgttagt tgtcctcgct
      601 ccatgcatgc ctaccattcc catataaagc tcatgagtgg caggaaacga ccctaaaccc
      661 ataagggtag ttgttacagg tatcctggct ttctccgcaa gaatacggac ctccttatca
      721 gcccctgcta tggatactcc gccccccgat aatattatcg gcttatgaga tctatttata
      781 gcctgtgcag caatttcaat atcattattt tttaatccaa tagaaatgtt atcaggcagt
      841 tttggagctt ccggctcata ttcagtaaca gcagcagtta catctttgca tacatcaatt
      901 aatacaggcc ctggccgtcc ttttccggcg atgtaaaaag cttcccttat tatacctgct
      961 agtttattta catcctttac tatataatta tgctttgtta tgggcatagt tattcctgta
     1021 atatcaacct cctggaaaga atcctttcca agaagggaag ttgctacctg tcctgtaaac
     1081 gcaaccatag gcacagaatc catatatgca gttgctattc ccgtaacaag atttgtcgcc
     1141 cctggccctg aggtagcaat acatactcca actttccccg tagcacgggc atatccgtca
     1201 gcagcatgag cagcaccctg ttcatgagaa gtcaaaatat gatttatttt atcctgtgcc
     1261 ttatataatg catcataaat atttaacact gctcctcccg ggaaaccgaa tacagtgtct
     1321 acgccttgtt ctcttaaaca ctctataata atttcagcac ctgttaactt cattaaatcg
     1381 cctccttctc ccgttgagta tcacccggta ccgtttctaa cctctatcct ctcacttcca
     1441 acttctaact tctaacctct cacctctaac ttaccactgc tcctctgctt gcagaggata
     1501 caatctttga gtaacgtccc agataaccct ttgtaatttt aggtgttggg gctttccaca
     1561 tactcattct tctctcaagt tcctcatcag ttatttctat atttatcttt ccacctggaa
     1621 tatctatact tataatgtct ccatcacaaa caacactaat aggtccgcct tccatagctt
     1681 caggcgaaac atggcctata gatgcacctc tggtggctcc tgaaaatctg ccatcggtaa
     1741 ttagtgctac atccttgtcc aggcccatgc ctgctatagc tgaagtagga ctcagcattt
     1801 ccctcatccc cggtccgcct tttggaccct catacctgat aatgagcaca tctcccttgt
     1861 agattttccc gttgtatata gcatcgatag cttcttcctc cgaattaaac acttttgcag
     1921 gtcctatatg gcagagcatt tcaggtgcaa cagccgaacg ttttactact gcaccttctg
     1981 gggcaagatt accttttaat actgctattc ctcctgttat accatatggg ttgtccaggt
     2041 ttctgattac ttctctatct aagacccggg atttttcaat attatcctct attgtacttc
     2101 ctgtaaccgt cattagtttt ttgtttatca aattcctgcc ggttaactct ttaattacag
     2161 cctgtactcc gccggctgca tgcagatctt gaacatgatg ttttcctgca ggagacaatt
     2221 tgcaaatatt aggaactctg ctgctgattt catttattat atccagattt atatttacac
     2281 ccgcttcatg ggctatagcc agaagatgca gtactgaatt tgtagaacat ccaagtgcca
     2341 tatcaacagt gagagcattt tcaaaagctt ccggtacaag tatatcagaa ggctttatat
     2401 ttttttctac aagctccaaa attttcatcc ctgttaactt agccagtctt attcttccgg
     2461 catacacagc tggaatagtt ccattcccgg gcaacccaag cccaagagct tctgtcagac
     2521 aattcatgga atttgctgta aacatgcctg aacatgatcc gcatcccggg caagcatttt
     2581 cttcgcaatc ccgaacttca tcctcagtca tttttccggc tttgtaagct cccacagctt
     2641 cgaatacact attaaggtca atatactcgc cattatgttt tattgagagc attggccctc
     2701 cacttataaa tatggaggga atatctatcc ttgctgcagc cataagcata cctggcacaa
     2761 ttttgtcgca gttaggtata aataccattc catcaaagct atgggcaaga gccattgctt
     2821 cacaggaatc agcaattagt tctctagtgg caagggaata tttcatacca atgtgtccca
     2881 ttgcaatacc gtcacatact ccaatcactc caaattcaac gggtgtacct cctgccattc
     2941 tgataccggc ttttacagcc tctgttattt tatcaaggtg aatatgtcct ggtataattt
     3001 cactttttga attggctata cctatcaaag gacgatttat ttcttcatca gtataaccca
     3061 tggccttaaa tagagacctg tgtggagctt tttctatacc acttttaatt atgtcacttt
     3121 tcataacaaa accctccgat ttattcctta taacttacgt caaaggaaaa tattatatat
     3181 tgacgtatta gtcagattat cataaatgca caaaactgta gtactccagt ttcgatagta
     3241 cttcagtatc ttcttcgagt tatatttgga ttactggctc atcattatta tgccttattt
     3301 tactacgcac tacttttata gtctataact ttacttaata aaagtttaat tcattaattt
     3361 atcggataat ttagataatt ttcgttaaac attcaacact tatattgtat gaacttatat
     3421 tttcatcttg ccgggaaaca tgaaatcaat aataaaattc ttttcaaaga agaattttgc
     3481 aaacattgat ttttctattg cttcataaac cggtctgaat gaaggtgttg tattaaaaag
     3541 cattaaaacc gcgcagacga tatatacagt aagcaaccct tccatagccc caagagcaaa
     3601 tcctcccagt ttatctatct gcttgaataa aggaagcttt gaaatgcctt ccagtaaata
     3661 tttacatata ataaggataa ttcttatgct tatatacaat aacagcatac ttaatatttg
     3721 gatgataatt ttagcaagtt ccccacttat agattccata acctttgtta tatcaataag
     3781 cttagcaggg tccggtatct gacttgtcaa attatttttt aaaaactccg gtagaggcag
     3841 cttgttaata accatatcag cagcggcttg ttttacctgt tcaccagctc catttattaa
     3901 cgcacctttc tgcttgagaa ggttatcaaa aattgcattt ttaatattct caaatattgc
     3961 agtattcatt aataattggg aaacttttgg atataattta aatgatagcc agattgatag
     4021 aaaatatgat gcaattttaa aaacagaaag cataaaacct gcgtttagtc caattaagcc
     4081 aaatccgata attattgctg ctacagcaag atcactccaa ttcatatgtt aacccccttt
     4141 acaataaatg tatctctttt atcaggttta tttactaatt tcttttaata ttgatttaat
     4201 gcaccctgtt ttatcatact tactaattat taataaatct tattttatat taattataat
     4261 tattctactt tttgtaatca gtgcagcttc atttttatta aaaaagatta ccttttctat
     4321 atcggtaata gaactgtatt ttccttccaa agttcctttg ctatttataa agtatacctc
     4381 tcttttattg ttaacagcag ctatatcttt aaatgtggaa atattacaaa cctgtccgtc
     4441 aataggacat ctggcagtta tcctgccatc cttgttgagt ataataaccc cggaaaattt
     4501 ctgtcttcca cccgtatcat tgatatcatc aacagcagca atgaaagact tatctgaaat
     4561 ctccttacaa ccatatatct ttttatactt attttcccat aacttttccc tattcttatt
     4621 ataatagata attgatacat catttgccaa agcaaaagaa tcatcgctta atgcaagtat
     4681 aaatgggtaa attgctccct gttccgggac taaagcagcg aaaggattcc ccatcaagtc
     4741 ggtaaaatct atgcatgtat aagcagtcga ccccgacgca tcaatgttat taataataat
     4801 ctgctgccct gaagccataa cctgggatga caaaacaaaa gtctcgttaa tgtatctgtg
     4861 gaatatctcc tggccatcta catcgtagac tgataccatc cccttataac cttcagtttc
     4921 atgtacaaca gacacatacc ctgtctcatt gatagatgca ttaattatat taccatcaac
     4981 tgctttctgc cattttatat ttttaccctc tattacataa atagaccttc caccaatatc
     5041 agataccagc agccgccccc cttgcgattg taacataggt ttattaagca atataggaat
     5101 actccactgt tcttctcctt ttttatcaat ggcagttacg ctgtataaag tgcattttat
     5161 aagccattct ctgtatactt caaatgaggg cttatcttta gcttcataac caatctcaaa
     5221 tatattcgca ttatctgcat caatttcatg tccctgtatg gcagcagaaa ctaacttctt
     5281 taagccaatt ttagaaatat caatattatt gctctttaga taagctatgt atgcagtaaa
     5341 cataatagca atcaaaagta atataaacaa aaatacacca gatttttttt cattagttcc
     5401 cattctgaat ttcccttcag tagtgtttac taaaaccttt tcattttgta tctaaaaaat
     5461 atcttatcac aatcggggac attcctcaat ataaggaaaa aaactccggt atgctaataa
     5521 atttcattaa taacataccg gagtttaaac tcatatctta acaaatgtct atgacattac
     5581 tttactattg tgctgtttca gctttcttca aagtctccat aagtttggca taactttctc
     5641 ttacaccttc aggttcgtcg aatttctttt tatcaacaag tattccgcta tattccccat
     5701 ttttgacaac atagtaataa cggtcgtctt ttggaataaa ctctaccttc attaccttag
     5761 gatctttttt cagataatag gtaaatgtta tatcaggctt tactgaaggt tgaccgtcag
     5821 gctgtacctg gctcagagta actccaataa gagactgata atatgacctg aatggctgtc
     5881 tgtcattttc gtctttcata cttgcatctt taccatttac aaagaattta tccttatctg
     5941 tatcctggtc agggtcagtc tgcaattcca aattcaaatt gtagccatcc atttcaacgt
     6001 tgattttttc aacatcccat atatttacta tataagcaaa cacttctact atctcttcta
     6061 taggtttatc caggaaatta aatggttcac tggatatggt atacacttca tttacaccat
     6121 ccagcattgc ataaacttta gcattttccg aaatttcctt acctaaaagg agacggtttt
     6181 ccccttggga agattcaaat tccaacacgt atgatggttt tgccaaccca tatttctcaa
     6241 gatccgacgg attttcctcc acaaactcag taatctgtga ttgtgttatg gcatcaagca
     6301 ttggcgacaa agcagaaata ttggcatttc ccttaattgg agaggtcagt gtccaattat
     6361 tttcttcgct gttaatattt gcttcaaaaa ctactgaccc ttttctttcc attgatattt
     6421 ttgttatatc agtagattca atggtgaata gtgctacatc tcttatatca tttttcccaa
     6481 ctattacttt attaattgta tatgtgtctg tggtataaac cttgctactg tctttctttt
     6541 ttacataata tgctcctcct gtcggtgtaa ggttaccaat ttcaagagtt tcaacctttc
     6601 cgtctttcat tttcgcagac actattatgg gcttgtcaag accatattgt tcaaagtctg
     6661 tggcattctc ctcaacttct ttttcaataa atactgaaga aaaatttatt gctatactgc
     6721 ttagtttgct tgaatcatac cttatacctt caggatactt gagctcccat tctttgtctt
     6781 tcttagcaat ggttaattta tcgcttccct tttgtacagt taattcttcc atttccgttg
     6841 tatcaagatc gattattttt tcaaagctct tgcgttctgt aacttcagtc ttgctttttt
     6901 ggtttttagc gaaggcatac actccaaaca acaatgcaag tataattact aaaattatag
     6961 cattcctgta aagcttcata gatgtctcct ccttaaatat acgaatacac cagccaggag
     7021 aattaacatt ggaacaacta ttactacaat aattcccata tattgtgcct gttctgcagt
     7081 gatattaaga tactgttgct ggtagttctt aggctttata atggtttcat cctttttatc
     7141 ctgcatccag atcatagtct ccaggaaaaa tgccatgcca ttgctgaaat atggtccaaa
     7201 agcatcctgt gcactgtcct cgagaaatat actgttacct ataacagcta tcttggaaat
     7261 tttttgccat cccttgtgtt caactgcaac agcaacatca agaggtccct ctatatcatt
     7321 tcccttggac ttgtccacct gttctcccac agctttgctg ctggttctgg caagaggtat
     7381 aacattaata tattccttgg tattcttcaa tatatttata cttctcgagt tggcaagaag
     7441 tatgttgtaa ttgtcagata ttacagcccc gctttttaca tccattaata ccacatatgg
     7501 gttctgaggc aaatgtctgt tttgatcgtt ttcttttacc ttatcataat tcaatgaaag
     7561 gttgtaatcc attaaaatat tttcaaattg cgggaactgt gggtccaatt caagataatc
     7621 aaacatgaat atagctttac cgccattttt caaatattcc ttaattttat ctttttcact
     7681 tgcagtaagg tcttttttag gtgaagcaat taccagaatc tccgtatctt caggtacatt
     7741 tgcttcagtt ataagattaa taggcttaac atcatagttg ttatactcca tctgagcttt
     7801 tagtgttgaa aaattctttt ctatgtcttt ttcatcatgg ctggttgtaa aataaattgt
     7861 gggagtaaat tcagcagtaa catattttat tgctcccgta aaaccctgct ccgctgtgga
     7921 tcctgtcttg taatactgga atgtttgttg attaaagtct gcactaaata ggtcataata
     7981 tgaaagcccc tttatccttc tgcctgactt tacaacaaaa gcatttttgc ttatatcttt
     8041 caaaacaccg tcggcatcaa tctgcttaag tataccagga ttcttatccg ggtctacata
     8101 ttttacagtg atattagggt attttgcgta atggtcaaga agttcaacaa cttccttgta
     8161 ttcgcttgag ggccctattt tgccatcatc aaacaatccg tatattacaa cttcctggtc
     8221 taattccttt aatatggttt tagtagtatc tcctatagag aaaagcttat taggagttag
     8281 atcccattta acctgaccca gacccactaa aatattcaaa actactgcaa ttgcaaccac
     8341 cgcagcaatt aaaataattg agtttgttcc gtatctgaac gatctgtttt ttacaacctt
     8401 attaaacgtc ttcttaacat tatttgttcc tttgtttttg gaattactat ttttaatatt
     8461 atttgtttct tcagcatttt taatattatt gtcattttca ttattgtcat tattgttttc
     8521 ttcattgttg catgctttgc tgatattatc agtttcttca ttaatgtccg cagttacagt
     8581 cacttcattt tcagcattat tattgatttt attcatctct tcatttttat tcatctatat
     8641 cacctctgac tccaacgtct tctttcaata atcatgatgg taacaaacaa aaatactgat
     8701 ataaaactca ggtaataaac aacagcactt aaactaagaa tacctctatt aaaatcctca
     8761 taccttgata gcagagaaaa ccaatacaga attttagctg taaatccgcc taccatacct
     8821 ccaataccat ctgcaatcca gattatcaaa agagccacaa aactgataat tgaagcaata
     8881 acctggtttt cggtaagtga agatgcaaaa atacctactg aaataaaaga tgctcccagg
     8941 agaattaagc cggcatatcc ccccactatc tgaggagtaa tggatgcgcc gaaaatcacc
     9001 agtattatcg ggtatataaa tgatacaaga atcatcataa gaaatacaga aagtgcagca
     9061 aagtattttc caagcactac accgcctaga ttaataggtg atgtaagcaa taagacttcc
     9121 gtcccgtttt ttctatcttc agcaataata cgcatggtta atataggtac taaaaatatt
     9181 aatatataac ccatactgcc aagatttccg ttaaattccc catatccgga taataaattt
     9241 ggccagaaca ttattgaaga tataagagta aacatgccaa tcaaaacata cgcaatcgga
     9301 gaataaaaat aagacttgac ttctttccag aaaacagccc gcatattaac ctacctcctt
     9361 ttcttctgtt actacctgca ggaatatttc ttccagggtc atacccaaag actttaactc
     9421 cattattggg tagcctgctt tagccattgc aaagaaaatc ggctttctca catctatatc
     9481 cttatcagat tcgataatat atgttataac atctttttcc ttttccgcgc tgggttcaat
     9541 atacttaaca ccatatattc cttttatgct gtttactacg gaatttttag gtcctgaaat
     9601 agtaacagac attttagaca tgttcccgaa gtttttggaa agattttcag gagtatctat
     9661 tgcagctatt tctcctttat ggataataac aactctctca catactgcgc taacctctgt
     9721 caaaatgtga gagcttaata ttattgtgtg ctctttaccc aacgctttaa taagcttcct
     9781 tatttcaatg atttgtttag ggtccaagcc tactgtgggc tcgtccaata caagtacttc
     9841 cggcttgcct attaatgcct gggcaaggcc tactctctgt ttatatcctt ttgaaagatt
     9901 tttgatgagt ctcttcctta catcagttat ttttacaatt tccataatgt catccagctg
     9961 accttttctg atttttttgt caaccttttt taattcactg acaaaattaa gataatcaag
    10021 aacagtcatg tccatataga caggcggctg ttcaggtaaa tagcctatcc tttttttggc
    10081 ttctataggt tgttcaagaa tatcatagcc gcagactttt acagtacctt gtgtggatgg
    10141 catataaccg gtaataatat tcatggttgt ggatttacca gctccattgg gtccgaggaa
    10201 accaagaatt tcgccttttt ctacggtgaa attcaaatcc tttacaccta ctatctgccc
    10261 ataatactta gtaagatttt gtatttctat cattcttttt tcttcccccc tgtttttcta
    10321 tactggaaca caaagctatt acctacatta ttatggatat tttttaacaa agtgtgaaca
    10381 atctgtaaca aaattttaat atctatataa cgcaaaaatg attttacaag gaacaagttg
    10441 gatttttgca tattaattaa ggtaacataa atgagcaaaa cttttatagt gtagtttctt
    10501 ttgtgcgtta tctatatcat taacaccttt tctttgtgaa gaagcttgaa cctacaggcc
    10561 ctatagcgct aaacccaata acaaacaacc attcatggtg agttaggggc actgtcttaa
    10621 atattccttg gagcatcggt acatacacca ccgataaaat cataaataat gaacagagca
    10681 ccgcaagcac tagataaata ttgctaaata cctgaatttc aaatatgctt cttctttctg
    10741 atttacattc aaaaacatgt attagctgag taataacaag tgtgacaaat cctcctgttc
    10801 ttgccagatc tatactgtgg gtaaaatgaa gtattgctgt aaagacagcc agggtactta
    10861 tcccaagcag ggttcctctt actataatca agctcagcaa tccatcagaa aatatattat
    10921 catttgaatc tctcggtggt ctcatcataa tatccttctc agcaggctca aagcttagag
    10981 ctattgccgg aagtccatca gttacaagat tgacccataa tatttgaata ggcagaaggg
    11041 ggacagggag ccctaaaagc attcccaaaa acattgtaag tacctcgcca atattacacg
    11101 atagcagata ccttataaac tttctgatat tactgtatat tactcttcct tcctctacag
    11161 ctgctacaat tgtagcaaag ttatcatcca tcaatatcat ggatgaggct tcctttgtaa
    11221 catcagtacc cattattccc atggatactc caatatccgc ttcttttaca gccggagcat
    11281 catttactcc atcaccggtc atagcaacta tatgcctaag ttttctgaag gcttttacta
    11341 tggataattt atttttagga gatactctgg caaatacaga aacattttct acctgtctta
    11401 aaagttcatc ttctcccatt gtttcaatgt catttccagt taacacctta tcttcttcag
    11461 aaaaaatatc aagttcttta gctatggcac aggcagttat tttgtgatct ccggttatca
    11521 ttacaggctt tatacctgcc atcttgcatt tttggacagc caaagctgct tcttgcctgg
    11581 gaggatcaat cataccaagc agtccaacaa atattagttc agattcaagg ttgctttttg
    11641 aaggatccat agaggtgttc atatttgata tatcaatttt cctgtatgca actccaagaa
    11701 cccttagagc ttccacagcc atattatcat ttactttaag aatccccatc ttcattatat
    11761 tggtaagatt aacaatacct tttgaagtaa gcattttaga gcatttttta attattatat
    11821 caggagcccc tttagtaaaa ataaattctt caccttttag gtttttacaa acaacactca
    11881 tacattttct ttcagaatcg aatggtatct catgatacct gtaataatcc ttttccaatg
    11941 attcctgtgt tataccggat ttagcagctg caacaagcag tgcaccttcc gtaggatccc
    12001 catttatagt ccatttctcc gtatggacaa acaccgactt tattttatta ttcaagcttt
    12061 ccttattatg acgtttttta attaattgtg aattattgca caaagcacct atctcaagtg
    12121 ccagctttag atgattgctt tttaagggtt ctatttttct tccctttaag ataaaatctc
    12181 cttctatatc atagccaatt ccggtaatgt catatatatt ttcaccggca tatatctttc
    12241 ttactgtcat tttgttttca gtaagagtac ctgttttatc agagcatatt acattcgcgc
    12301 atcctagggt ttctactgcg ggcagcttgc ggatcagtgc atttctcgtc aacattctct
    12361 gaacgccaag tgcaagtgat atggttacta tagcaggaag gccctccggc acagcagcaa
    12421 ctgccaggct tattcctgcc agaagcatgt taaatacttc ttctcctctc ataataccgg
    12481 ttacagaaac gacagcacat attacaaggc aggcaaaaac tataaacttg ccaaggtgtg
    12541 ccagcttttt ctgaagaggt gtctcctcat catcaatatt ctgaatcata tgagcaattt
    12601 tacccatctc ggtattcatt ccagttgcat ataccatggc ttttcctctg cccattgtta
    12661 atgtggtacc catatatata tgcctgctct tatcactgaa atcaagctct gaatttatat
    12721 agtcttcatg ttcacgtttg aattcgcgtc catgataaat atcaatattt tgttccttaa
    12781 ttattttctt tccattattt cttctatgat gtttctcaac tggaacagat tcgcctgtta
    12841 ataatgactc atccacatta agatttgctg attcaataag gattgcatcc gctggaaccc
    12901 tgtcaccggc ctcaacaaga attaaatctc cgggaacgac tttatctgct gaaacagtaa
    12961 caggttttcc atctcttata acttttgcag aaggagcagc caggcttttt aaagcttcaa
    13021 gtgttctttc agttctgtat tcctgtatga aaccaagaaa agcatttact attacaatag
    13081 caagtattgt cattgcttcc attgtttctc ccataaaagc tgaaatagca gtggctgcca
    13141 atagaattaa aaccataaaa tcactgaact gctcaactaa aattttaaag ggagatattc
    13201 cggcttttcc agccaaacta ttaggcccgt atttgttaaa cttctccctt gcttctcttt
    13261 cacttagtcc tagactctta tttcttcctt ctttgactga tggaaaatct tcagacaata
    13321 aattgtcctt tttcaaaacc gatttcaagt gaatcaaacc tcctttttcc tgtccttcct
    13381 catattctta ttcacttaaa aacagtttta gtacaggtct tattattgac tgcatattat
    13441 tgattgatta ttctgattat gttattgata gcctagccct gattatggcg gtgcattagt
    13501 gcataaaaat tatgtatatt atttttcata agccttataa actccctcaa tattgcacaa
    13561 gtcaatcata atatcttcta cacggaatgc atgaggaggt cttacaaaga atttgacagt
    13621 catatttcca tcnnnnnnnn nntaaaatac tcaatattct taatcattac ttcatacctg
    13681 caaaatatat cagttaattt atatattatt tcaggtattg ctgcagtttc tacaaaaata
    13741 tttttatact ggcttcttac attaaactga ctttctatct tttttagcat tattaatgta
    13801 atatatatta atactgttgc tgtgattgct ccttcataaa accctatgcc cgcagcaagt
    13861 ccaacacagg atactgccca aagactggcc gcagtagtca gacctttcac attaaatcct
    13921 tctctgacaa ttgtccctgc acctaagaag cctataccac ttataacctg ggcacccaac
    13981 cgagcaggat ccaaattcac gataggggaa aacagcttaa aaatatattc agaagtagtc
    14041 attacaaggg ctgagcctat acaaacaagt atatgtgtcc taaatcctgc aggtctgttt
    14101 tcatgttccc tttcatatcc aatggctcct cccagcagta aagctaatac caatctggct
    14161 aatatttcta gaaaataagg catactatcc actccaaaat attattataa cttttgctaa
    14221 taatttctgt tatcaattat gtctttatct gttatatacg taatttgtaa ttttatttct
    14281 gtcctgccat tcttgataat tattcacaat tttcaagttt tcatttaaaa tattccatat
    14341 caccgctgct tcatcacttc tcctccaggc ggctgtacct gcaagtttat atttgtgtac
    14401 caaggatgat ttaagattaa ttgagttttc atcctcaagc catattttgt atacttttcc
    14461 atccttattg aattgactat agaattgtcc actttcctca tcccatttaa ccgtagtatc
    14521 attttctgca atcatttttc tggcattgtc catagaaagt actttaggat ttgttacctt
    14581 tactcttccg cctttcactg tttcttcctg ccagagtctg gtataaaacg gaagaccaag
    14641 taaaagtttt tcttttggaa ctatttttaa cattttaaca atgttgtttt ctacccagct
    14701 taactgtgcc acagaacctg ctacaggact tgaactccaa tgctggtcat atgccatcag
    14761 cataacaaag tcaacaattt ctcctataga agtgcgatca tagcattttg accaggtatc
    14821 acttccatcg ggaatggtaa catccatgga tacaaccagc ccctgttcct taaggaaagg
    14881 ggtgatctct cttacgaact gggttagtgc atctttgtct tctttatata tattttcgaa
    14941 atcaatattt attccatcca gtttatacag ggatgcatat gtcagaatct gtcttatggc
    15001 gttatctctt gcatcagtat tattcaaaaa cctgcttgag gcctttatat cttgaaaatc
    15061 attactaaaa agggcccata ttttataacc gtttttatga gcccagtcta cataggttgc
    15121 gtctgcccta ttaattacgt tgccttcatc atcttttaat tggaaccatg taggtgaaat
    15181 tacatcaagc ccttcaattt taccaatttt acttaagtca ggcctttgtc cgtacatcat
    15241 ttcccatacc agattaattt tccctgcttc cgggttccat aactccattt ctttttccct
    15301 aggtaatata ttcaagttta ctgtaagcca ttttacagat acaaactttt tctctatata
    15361 acctatttca ccttcttgag ttctaacttt ataccattta tcatattcct taaatattct
    15421 taataaagtt ttaccactat tgccattatc atcatcaacc ttatagctat tagtttcatt
    15481 atttatttca gtaaattgta acttttttat tatcggatga ttttttgatg ggcctttacg
    15541 tattacagct ttagggctta tgggttctgc aatctgcacc atgctgtatc ggggatcaat
    15601 aataataaca ttatcagact taatgtagtc aatgtcaata ttataaaaat cacttagaaa
    15661 ctcaattggt atatacactt ctccattttc ctcaatagca ggtatattaa ggttaacagg
    15721 tttgttattt acaagggcct caagttcgtt tgttttcatc cttataaccc ggtcttttgt
    15781 tgttattgtg acttttttca aactctcatc ccaataaata gtcttatcaa aatatttttt
    15841 aacaatttct aaaggtagta aaatttcatc atccataatc ttagggggat actttgaaac
    15901 aacttcctca tcttcgataa caagatgaag accttctgtt tcaaaagcag ccgtaatact
    15961 gttatttggc ataaaaaaga aataatacgc tgaatacccg aaagccccta taactgccaa
    16021 tactatagta accaaaatcg tccttaaaaa agctttcttc attaacattg acccccatgc
    16081 tacaataatt taactataga tagtgcgtaa aagaaactac actataaaag ttttgtgcgc
    16141 ttatattacc ttaattaata cgcaaaaatt caatttattc cttgtaaaat catttttgca
    16201 ttatatatag atcaccggaa agaagttctc tgcttctcta tcatatttcc atctttctcg
    16261 gtatgaatgt cttaaaggaa ttaagcatct tctcatccgg ttcaaagaaa accgcagtct
    16321 tttcaccttt atataaaaga gttgcaaaaa atacattttc agaatccatg gaacttacag
    16381 cttctattct gtttttgata tcttgaatcc tccggtcgtt atttccacct tttagtcgtg
    16441 ataccatatc aaagtccttg caatttgcac taaatattct tttccttctt ctctgagcta
    16501 tgattttatc tatatccagg tcaccatttg tcacgatata ctcaaattct atatttcttg
    16561 atattacaaa ctgataaata aaatatacca aagctgcaac aataactata aaaaacggtc
    16621 ttaaaaaagg tataagctga agtacaaaaa agagtacaag ccctccaaga acaactcccg
    16681 ttatgataag actgtcttta gcagttcttt tttttacaac aattttttct ataaaactat
    16741 ccattaccaa ccccaacctc ctattttcaa atatacttga ccaaaaacct tgaactaaat
    16801 tttatcatac attttcaata taaacaatat gttaggaaaa catagggggt gcattttcta
    16861 tatttgaggg aaaatatggc aagaaacgat ttccatgtat aatattcatt tcagcaacgg
    16921 attgtagagt ttaagtctac agaaaaaaca ggtcaggaat tttgcatctc tgacctgttt
    16981 tttactactt gcttaatatt gctttactat cttttatttt aggctttttt aatacattcc
    17041 accgcctggc attccgccac ctgcaggcat cgggggttct ggttcgggta tgtctgcaac
    17101 caggctctct gtagtaagta ccatggctgc tactgaagcg gcattctgca acgctgttct
    17161 tgtaactttt gcagggtcta caataccggc ttctaccata tttacatact tctcattcaa
    17221 agcatcaaaa cctatttctg gttcgctgtt tttaactttc tcaacaacga ttgaaccttc
    17281 aagtcctgcg ttaccggcaa tttgtcttat aggctcttca agtgctctta caattatctg
    17341 cacgcctgtc ttctcatcgc ctgctacttt ttcaagaagt tcctttacct taggaataac
    17401 atttatatat gcaactccgc ccccaggtac aattccttct tctacagccg cttttgtagc
    17461 tgcaagagcg tcttctattc ttaatttctt ttctttcatt tcagtttcgg tagcagctcc
    17521 aacctgtata acagcaactc cacctgacag ctttgcaagt ctttcctgga gtttttctct
    17581 gtcaaaatca gaagttgttt cttctatctg agctttaata gcagcaattc tcttctttat
    17641 ctcttcaggt tttccatatc catcaactat tatagtattt tccttttcaa ctttaacctg
    17701 tcttgcacgt ccaagctgag acaattcagt atcttttaat tcaagaccca gctcttcgct
    17761 gataacttct cctcctgtga ggattgctat atcacggagc atatcttttc ttctatcacc
    17821 atatcccggt gctttaacag ctacacaggt aaatgtccct cttaatttat tcactaccaa
    17881 agtagccagg gcttcacctt caacatcctc agcaataatg acaagcttct tgccttgctg
    17941 aactattttt tccagaacag gcagaatatc ctgtatattg cttattttct tatcggtaat
    18001 aaggatg
//
LOCUS       DAOSDD010000069        17965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1272_length_17965_cov_4.701619, whole genome shotgun sequence.
ACCESSION   DAOSDD010000069 DAOSDD010000000
VERSION     DAOSDD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17965
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_17965_cov_4.701619"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2712
                     /gene="carB"
                     /locus_tag="PK733_08005"
     CDS             <1..2712
                     /gene="carB"
                     /locus_tag="PK733_08005"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ10521.1"
                     /translation="WEELEVEVVRDSKNQMAAVCYIENVDPLGIHTGDSFCVAPMLTI
                     SLGLQERLKNYAFKIVENIEVIGGTNVQFAHDPVSDRVVVIEINPRTSRSSALASKAT
                     GFPIAAISTILATGMTLDEIPYWRDGTLDKYTPSDDYVVVKFPRWAFEKFKGSYDKLG
                     TQMRSVGEVMSIGKNYKEALQKAIRSLEIGRYGLGFARNYNQKPLNELMELLHETTSE
                     RQFVIYEALRKGAGVEDICRLTNIKPWFIEQMKELVMLEEEILKYKGYKVIDGSNKGI
                     GNNIGSSNNSRNNSTGSLNIPDNLLIQAKKDGFSDRYLANLLGVSEGEIRNRRIALGL
                     SGAWEAIPVSGVVDAAYYYSTYNTSINGIDNNTDNSIDKNPVTNNQKVMVLGGGPNRI
                     GQGIEFDYCCVHAAFAIRDMGLETIIVNCNPETVSTDYDTSDKLYFEPLTTEDVLNIY
                     HKENPLGVIVQFGGQTPLNIAAELAREGVCILGTQPDVIDLAEDRDLFRHIMEKLDIP
                     MPASGMASNLEQALEIANQIGYPLMVRPSYVLGGRGMEIVYDEEMLKQYVAAAVDITP
                     ERPILIDKFLENAIESEADAISDGVNAYVPAVMEHIELAGIHSGDSACVIPSITIPKK
                     HLDTISEYTRMIGEKLNVIGLINVQYAIADDKVYVLEANPRASRTVPLVSKVCNVQMA
                     KLATEIMVQGRVHCKPVSLDIAPRIIPHYGVKEAVFPFNMFPEVDPVLGPEMRSTGEV
                     LGLSKSFGIAFFKSQEATQNILPTSGTVLISVAEQDRPDVLEVAQRFNKLGFKIKATR
                     GTQAFLAENGIEAEIINKLFEGRPNIIDGIMNREIQLVINTPLGKRSQADDSYIRKAA
                     IKHRVTYITTLAAALASAKGIAAYRENSLDRDTVKSLQSYHNDIKEV"
     gene            complement(2927..3739)
                     /locus_tag="PK733_08010"
     CDS             complement(2927..3739)
                     /locus_tag="PK733_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10522.1"
                     /translation="MTGKDIKMNRLFSKGENAVIVAIDHGYMDGPIPGMENLNKTVEK
                     IDSCIDGILLSPGMLKNIGHAFAYKGAPMPIVRLNWSTVFCFEWGYTKAYARQAFSVK
                     DAVALGAEIVLVSLTLKTGDEKTDIENVELFGKLCNEAREYGIPVIGECFPNNSDNIS
                     QEEMHDQVLRGCRILAELGADMIKTFYTHKFTDVIEGCPVPVFGLGGHTTPNPVDSLI
                     LAQKEIKEGAKGVVFGRNAIQRPDPIAYQKALCEVVKHGMSPEEAVKKFGLK"
     gene            complement(3897..4937)
                     /locus_tag="PK733_08015"
     CDS             complement(3897..4937)
                     /locus_tag="PK733_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10523.1"
                     /translation="MSRLFEVRPVDKRFYEDKLKDFLPEKIIDIHTHVWLDKFRIKAK
                     DAPIRSATWPSRVAKDNSIEDLIETYSLMFPDKQVTPLIFAYPKVEFDIDISNKYINE
                     CSDKHGFPSLALAVPEWSGLELEEQIIKGGFLGCKVYLNFSKSYIPQNEIRIFDFLPP
                     HQLEVLNKHGWVAMLHIPRSKRLKDPVNLAQLLEIEQKYPQVKLIVAHVGRAYCPEDV
                     GNAFEVLSNTKNMVFDFSANSNKWVFERLIEAVGPKRILFGSDMPILRMRTRRICENG
                     NYVNLIPRGLYGDVSGDSHMREVDGTESEKLTFFMYEEIDAFRCAANATGLSGDDTKD
                     IFYNNAVRILGL"
     gene            complement(5267..6748)
                     /locus_tag="PK733_08020"
     CDS             complement(5267..6748)
                     /locus_tag="PK733_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ10524.1"
                     /translation="MYVIGIDVGTTGTKAMLINDKGIVLKSAYRGYGIIKSGRNMVEQ
                     KAEDWWNSLVETVKECIQDVNDRYNILALSISSQGGSLVPVDKEGNPLSNVIVWMDKR
                     GIDQQNDLLNLHPGEFYYKKTGWKLSPGGNLIQIKWLKENASDLFEGTYKFLSTLDFI
                     NFKLTGRYVIDPTNGAMTHLIDINRGLWDEELLSAAGINEKKLAEVMEPGLAVGELAA
                     SAAEELGLGTTVKVINGGHDQYCAAVGAGAIEEGELFLSTGTAWVLLGTFKQPVFDLD
                     TYIAPGRHIVNNLWGGLVSVPTGGVSMEWFKDNFASESFRDIDNKASLLMKRAENVFF
                     YPYFNGSGYPNYNPNVKASLMGLGLEHDRYDMALAIMEGVAFEINHAIEEFRNKGCKT
                     GHLRMLGGGSKSNLWTEIIANVTGAPVLRFKETEAACIGAAIIAGVGCGIFPDYRDGY
                     TSIMREEILTEADKNKSEFYREKYAMYKQGIKFIEEYYNKIFN"
     gene            complement(6940..7725)
                     /locus_tag="PK733_08025"
     CDS             complement(6940..7725)
                     /locus_tag="PK733_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ10525.1"
                     /translation="MEDEKLIEYGTEERRNKILEILTRQGKVKVNQLSRLFEISEVTI
                     RSDLAKLESMGLLERVHGGAVSTYRAYYRMSISDRMKANAEEKRRIAAEVASMISDDD
                     TLMISSGTTTLYVLEELKNAKNLTIVTNAIYFSQESANLRDFNIILLGGILEPRDQFT
                     YGDDVINQVKRYKADKFILSVDGINSEDGISTYHYLEAEINRQMIARANKTIVVGDYT
                     KVGRTSFAHISPLENVDVIVTNKNANEDEINEIKEKGVEVRQV"
     gene            complement(7888..9018)
                     /locus_tag="PK733_08030"
     CDS             complement(7888..9018)
                     /locus_tag="PK733_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10526.1"
                     /translation="MDEIRVGIVGFGFMGKTHAYGYKTIPLYYSNLPFKIKLVGVCDA
                     VPEVAQKAKESLDFEFATSNPDDIFSREDINVVDICTPNIYHKEAVLKALKSGKNVYC
                     DKPLAVSYEETKEITDVLDQYDVITQMALQYRFFPATMRAKELIDEGKIGKIMSFRAC
                     YLHSGSVDSKKPIGWKQEKKFGGGGVLFDLGSHVLDVMYYLLGEYSSLFAETKIIYPE
                     RPDKSGNMVKIEADDVAFMMVKMKSGGIGTIEASKIATGTNDELRFEIHGDKGAIRFN
                     LMEPNWLEYYDNTLPDAHYGGLKGYTKIECVQRFKKPGGDFPSSKFSIGWIRGHVHSL
                     YNFLSCVYEKRQASPSFWEGAYIQHVMEKAYESDQKKSWIEL"
     gene            complement(9178..10530)
                     /locus_tag="PK733_08035"
     CDS             complement(9178..10530)
                     /locus_tag="PK733_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ10527.1"
                     /translation="MYDKNWAEVDAKSLPAIKTELPGPKSREMHSRAAKYMKGYSSQV
                     RLFPVVFDSGKGCTLTDVDGNVFIDFSSGIYVTGLGHCHPKISEAVATYANKLMNCHD
                     FTTEIKMKLLEKLASITMGDLKGIQLYDSGTTAVEAGLRCARAATGKFEFVSFWRDFH
                     GKTMGAVSCAGVDKSQGMRAPGFFLAPRPNCYRCPFKMKHPECNCFCVEHLENVIKNE
                     TTGQVAAIVLEPIQGWGGSVVPPDDFMPKLRQLCDKYGMLLFADEVLTCMGRTGKMFC
                     MEHWNVTPDITTLGKGFGNGFPVTAMCVSDKYKDNIEKISASSSYGGNPMACAAALAS
                     IEVIEDENLCERSAHLGEVFLNRMNKMKEEHKIIGDVRGKGCLLAMELVKDKSTKEPF
                     DEAGKLVYQRAFSKGLAWVPAGHILRLSPPLIMDDDLALKGLDIIDEAVGEVEKELGY
                     "
     gene            complement(10887..12155)
                     /locus_tag="PK733_08040"
     CDS             complement(10887..12155)
                     /locus_tag="PK733_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10528.1"
                     /translation="MNVSQKKIKVGVFGARRGMTMINVLANHPDAELVAICDKSEYLQ
                     GKCEKIARDSDSKISLYSHFNEFFNHDMDAVVLANYANQHAPYAAKFLLSGRHVLSEV
                     LPVQTLAEAVMLVEAVEKSGNIYSYAENYCYFPATQEMKRLYRLGEIGEFMHGEGEYV
                     HDCESIWPSITYGDKRHWRNNMYSTYYCTHSIGPIITVTGTRPVVVVGFETPNVENMA
                     ALGYKGGTSGMIVMQMNNGATAKSLHGNLKREPGSIWYSIYGKKGMMETDRWHEGISR
                     INVFKEGNNLTPYETSYSPAPRAASELAKRTLGHGGSDFYTVHYFLERILGRSDGEEC
                     IDIYQALDMGIPGILAYRSICNGNIPIAVPDFRDKTIREKYRNDNWCTDPEVAGDNTA
                     PNCSFGSPDIPDNVYDKVRDTWEKSIKGKG"
     gene            complement(12816..13844)
                     /locus_tag="PK733_08045"
     CDS             complement(12816..13844)
                     /locus_tag="PK733_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOJ10529.1"
                     /translation="MPTLFNEVLDKRQIFERVGSLSQICGPRSYIMADGRAKGMSAVD
                     IRTGGGLNYTVLPDRGMDIAWAEFKGVPLGFISKSGVISPEYFCTDKTTWGESFYGGL
                     LTTCGLMQAGKGCMYHDRKFSPHGKVSNVPSEKTGIIYNWENDELVMGIRGILNEAQL
                     YKENLQMTRTITSKLGENKIIIHDVVENNGFEKVPLMIIYHMNFGYPLIGKDTELLVS
                     SKEIKPGVETSEADLLNYNRFDNPSVEYTSKMFYHNLETDENGYAICRLLNRKLKTCV
                     SIKFNKNELWNFMEWKHLKAGDYVLGLEPCNNYAMGLAEEEENETLEYIEPGVIREFT
                     LEIGVTEE"
     gene            complement(13923..14879)
                     /locus_tag="PK733_08050"
     CDS             complement(13923..14879)
                     /locus_tag="PK733_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10530.1"
                     /translation="MRKLKELKREGSSYKLSYFFIKIKRIKYWSNFIYRLGIYILLFG
                     LGLVFIFPFLYMIVTSLKSPDDLIDITVRWIPKTIYWSNYLVASGALKYFTYLKNSIF
                     VTVLCVIGHNISCSYIAYGYSRFKFKGRDFLFMFVLLSIIIPPEVLVTPLYIQYSKFR
                     WINTYLPLIVPSFLGFGLRGGLYIFIFRQFFLGLPKDLENAALIDGCGCIQTFWRIIF
                     PVAKAPILVSSVLSMIWHWNDSFEATLYLTSSKKWFLPTMLPKMYDTLKSLQPDDMMA
                     AIFYNEGVIMAGTFLTVLPLLLAYLFLQRQFMEGIERSGLVE"
     gene            complement(14893..15774)
                     /locus_tag="PK733_08055"
     CDS             complement(14893..15774)
                     /locus_tag="PK733_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ10531.1"
                     /translation="MNNRRISLRGRTLRVSQGYLFVFPWIIGFLLFFVYPLVQSIHLS
                     FSKITSIKGFKMEWVGAANYIKAFVWDINFVPMFISVVKSTLVNTPIIIVFSLFIAIL
                     LNKKIKFRAFFREIFFLPVVLGSGFIMQELIDMGVQQQVIDLSKIILLPQQVLQYLGS
                     NFSAIIQNYLNLITLLLWKSGVQILLFLSGLQSISKSLYEAARCDSATEWEMFWKITL
                     PMISPIILLNVVYTLVDSFTDINNPIVTYLVNVSIKQFNLEYGAAMGWIYFIFIFIMI
                     ILMFILSKLFIHSSYER"
     gene            complement(15771..>17965)
                     /locus_tag="PK733_08060"
     CDS             complement(15771..>17965)
                     /locus_tag="PK733_08060"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ10532.1"
                     /translation="NSMKSKVIAMIITIILCIIFIIGVRSTVFGSQVIDKEKYKEYKI
                     ISETKYLELYYSYENHSIMVHDKRSGDIWFSMANQEKHGTENINAFTRTSMQSLFSFT
                     YNDFKNESKESSKLSNSVLEESEVSEKDIENGISISYNFTKLGINATVDIWIDDNSLY
                     AKVPVEKIKETGKYGLMNIELMPFFGAADDNENGYIFYPDGCGAIMHFGENPNIYANK
                     YRWFVYGTDKVDIDSYEAYEQKKQEQAMLPVFGMIIGNSGYLAIIEDGACDTAINLCP
                     SGHIVNLNRIYPEFTYRRSFKDPRPNVENASKVEKEIIKADHSVRYIFLNHDEAGYSG
                     MANVYRNYLIESNGIKRNISVQEDIPLALELFMGIKERNLLFDRYIAMTTFDQAQEIL
                     DTFKNQGIDRIQVSLKGWTKNGYGILNAHLPPNRLLGGSSGLKRLVEYTSENDIQLLL
                     QDNFFNLQKGAGTFVGRYDIAYQMYKMVVTDRKKEYFLYNPVNAFEKFRNYLFDINKY
                     RINGLQLEKVGQLLYYDYNIKYPVIRQNTADYWCKMIEESSNKLGFTAVHGGNAYILP
                     YANRLCNIPVEDTGYFITSEQVPFYQMVVHGLIPYTSSNPANLFYDYPREKLKWVEYG
                     CMPYFELTYNEAELLRYTDYNKNFSSCYEDWVKTAVDIYKEFNERLGDIWSKNIIEHK
                     KLNQGLYSITYEDGSVVYVNYNEQEASINEHKIKALDYLVVDRRGEER"
BASE COUNT         5409 a         3812 c         2782 g         5962 t
ORIGIN      
        1 tgggaagaac tggaggtaga agttgtccgc gactcaaaga atcagatggc tgctgtttgc
       61 tatattgaaa acgtagatcc tctgggaatt catacaggtg actctttttg cgtagctccc
      121 atgcttacca taagcctggg acttcaggaa aggcttaaaa actatgcttt taaaattgtg
      181 gaaaatatag aggtaattgg tggaaccaat gtccaatttg ctcatgaccc agtaagcgac
      241 agggtggtag taatagaaat taacccacgt acttcaaggt cgtctgctct tgcctcaaaa
      301 gcaacaggtt tccccatcgc agcaatatcc acaatactgg caacaggaat gacgctcgat
      361 gaaataccat attggcgtga cggaactctg gacaaatata caccttcaga tgattatgta
      421 gttgttaaat ttccaagatg ggctttcgaa aaatttaaag gttcttatga taaacttggg
      481 acacaaatgc gctctgtagg tgaagtaatg agtataggca aaaactacaa ggaagcactt
      541 cagaaagcta tacgctcatt ggaaatcgga cgctatgggt tagggtttgc caggaactac
      601 aaccaaaagc ctttgaatga attaatggag cttctgcatg aaacaacaag tgaaagacag
      661 tttgtcatat atgaggcatt acgtaaagga gccggtgtag aagatatctg cagattaacc
      721 aatataaagc catggtttat tgagcaaatg aaagaactgg taatgcttga agaagaaatt
      781 ctcaaatata aaggctataa agtaatcgat ggcagtaata aaggcattgg aaataatatc
      841 ggcagcagca ataacagcag aaataacagt acaggctctc tcaatattcc tgacaacctg
      901 ctgatccagg caaagaaaga tggattctca gaccgctacc ttgcaaatct tttaggagta
      961 tctgaaggtg aaataaggaa ccgccgtata gcacttggat tatcaggagc ctgggaagca
     1021 attcctgtta gtggagtagt agacgcagcc tattattact caacatataa tacctctatt
     1081 aatggtattg ataataatac tgataacagc attgacaaaa acccggttac taataatcaa
     1141 aaagttatgg tgcttggcgg cggaccaaac cgtattggtc aaggcattga attcgattat
     1201 tgctgtgttc atgcagcttt tgcaatacgt gatatgggac ttgaaacaat tattgtaaac
     1261 tgcaatccgg aaacagtttc aacagattat gatacttcag ataaattgta ttttgaacct
     1321 ttaactacgg aggatgtact aaatatttat cataaagaaa atccattagg tgttattgtc
     1381 cagtttggag gacagacacc tttaaatata gcagcggagc ttgccagaga aggcgtatgt
     1441 atcctgggta cacaaccgga tgttattgat cttgcagagg atagagatct tttcagacat
     1501 atcatggaaa aacttgatat acccatgcct gcctcaggta tggcaagcaa ccttgagcag
     1561 gctcttgaaa ttgcaaatca aatcggctac ccgttaatgg tccgtccttc atatgtttta
     1621 ggcggccgcg gcatggaaat tgtatatgac gaagaaatgc tcaagcaata tgttgctgct
     1681 gcagtcgata taactcctga acgcccaata ttgatagaca agttccttga aaatgctata
     1741 gaatctgaag cagatgcaat atctgatgga gttaatgctt atgttcctgc cgtaatggaa
     1801 catattgaac ttgcaggcat acactcagga gactcagcat gtgttattcc atcaataacc
     1861 atccctaaaa agcacctgga tactattagt gaatatacac ggatgattgg cgaaaaactt
     1921 aatgtaatcg gcttaataaa tgtccagtat gctattgctg atgataaagt atatgtattg
     1981 gaagcaaatc caagggcatc gaggacagta ccgcttgtat ccaaggtatg taatgttcag
     2041 atggctaagc tggcaacgga aattatggta cagggcagag ttcactgcaa gcctgtatct
     2101 ctggacatag caccacggat aattcctcat tatggggtga aagaagctgt attccccttt
     2161 aatatgttcc cggaagtaga ccctgttcta ggacccgaaa tgcgttcaac aggtgaagtg
     2221 ctgggattgt caaaatcatt tggaatagcc ttctttaaat cccaggaagc tacccagaat
     2281 attctgccaa cttcaggtac cgtattaata agtgtagctg aacaggatcg accagatgta
     2341 ctggaagtag cccaacgctt taataagctg ggattcaaga tcaaagcaac cagaggtact
     2401 caagccttcc ttgctgaaaa tggaatagaa gctgaaataa ttaacaaact ctttgaaggc
     2461 aggcctaata ttattgacgg tattatgaac agagaaatac aacttgttat aaatactcca
     2521 ttaggaaagc gaagccaggc tgacgattca tatataagaa aggccgccat aaagcataga
     2581 gtaacttaca tcacaaccct ggctgcagct cttgccagtg caaaaggtat agcagcctac
     2641 cgggaaaatt ccctagatag agatactgtt aaatcactac aaagctatca taacgatatt
     2701 aaagaggtat agtttaaaac tactatgcca atttgaaaag cattggtaac ataacgaagt
     2761 agtattaatt tactataact catgatcaag cagcacctgc actttataaa gtacaggtgc
     2821 tgctttttgt gaaacaatgt aagcagatac ttaaaagaga taatccgtat tgctgccaat
     2881 atagattatc tcaatcatat attactttta ttacttaaac caaatattac ttcaatccaa
     2941 actttttcac tgcttcttcc ggtgacatac catgttttac aacttcacaa agtgctttct
     3001 gatacgcaat tgggtcaggc cgttgaatgg catttcttcc aaatacaact ccttttgctc
     3061 cttcctttat ttctttctgt gcaagaataa gagagtcaac gggatttgga gtagtatgtc
     3121 cacccaatcc aaatacaggt accggacatc cctcaattac atcagtaaat ttatgggtat
     3181 aaaatgtttt tatcatgtcc gcgccaagct ctgcaagtat tctgcatccg cgaagaacct
     3241 ggtcatgcat ttcttcttgt gaaatattat cactgttgtt aggaaaacat tcgcctataa
     3301 caggtatacc atattctctt gcttcgttgc aaagcttacc gaaaagctct acattctcta
     3361 tatctgtttt ctcatcacct gtctttaagg taagtgaaac caaaactatc tcagcaccca
     3421 gtgctactgc atcttttaca ctaaaagcct gtctcgcata tgcctttgta taaccccact
     3481 caaaacagaa taccgtactc cagttaagcc ttacaatagg cattggtgcg cctttataag
     3541 cgaaagcatg gccaatattt ttcaacatac ctggacttag tagtatacca tctatacatg
     3601 agtcaatctt ttctactgtt ttgtttagat tttccatacc aggtatggga ccatccatat
     3661 aaccatggtc aatagcgaca attactgcat tctctccttt tgaaaaaagt ctgttcattt
     3721 tgatatcttt gcctgtcatt attaattcct ccaattatta tttattagtc caatcgtttt
     3781 gtaatcttta ttattggaac gagtaaatgg caagaaaact tgccctcaaa gtgtcctaga
     3841 tgcaagaaat gctgttcctc ctgcacgagc ccgcttttcc gagcagactt ccgagctcac
     3901 agccccaata ttcttacagc gttattataa aaaatatctt ttgtatcatc cccgctcaat
     3961 ccagtagcat ttgcagcaca cctgaacgca tcaatctctt cgtacatgaa gaaagtcaac
     4021 ttttccgact cggttccgtc aacttcacgc atatgactat ctccagaaac gtccccatat
     4081 aaccctcttg gaataagatt tacataattt ccattctcac agattcgcct ggtgcgcata
     4141 cgaagtattg gcatgtcgct tccgaaaagt attcttttag gtcctacagc ctcaattaac
     4201 ctttcaaata cccatttatt tgaatttgca ctgaagtcaa agaccatgtt cttagtattc
     4261 gaaagtactt caaaagcgtt tcccacatct tcgggacagt atgctcttcc tacatgagct
     4321 actatcagct tgacctgagg atatttctgt tcaatttcaa gaagctgtgc aaggttcaca
     4381 gggtctttca gcctcttgct tcggggtata tgaagcatag caacccaccc gtgcttattc
     4441 aatacttcaa gctggtgggg aggcagaaaa tcgaaaatcc taatctcatt ctgcggtata
     4501 taggatttgg aaaaattaag gtatactttg catccaagaa aaccgccttt aataatttgt
     4561 tcctcaagtt ccagtccgct ccattccgga acagccaggg ctaatgatgg aaatccatgt
     4621 ttatccgagc attcatttat gtatttattt gatatgtcaa tatcgaactc aacttttgga
     4681 tatgcaaata ttaaaggtgt aacctgttta tcaggaaaca ttaagctgta agtttcaatc
     4741 aggtcttcta tagaattatc ttttgccaca cgagaaggcc atgtggccga ccgaattggt
     4801 gcatcctttg cttttattct aaatttgtca agccaaacat gggtgtgaat atcgataatt
     4861 ttttcaggaa gaaaatcttt taacttatct tcataaaatc ttttgtcaac tggtctaact
     4921 tcaaataacc tgctcatcag taacctccca gacgtcaaat cttaattatt tcattcttat
     4981 tagcattact ttttctaaaa tagtatatag ggggtgcatt tttttatatg aagaaattat
     5041 tggtaagggg cataagtgga agatgctgta gagcctttta atctgagtga aggaacagca
     5101 aaatcagtgt ttgagcaaaa taactacaac tatcaaatta ttatttatca agttgttttg
     5161 tgagtttgat tttgcccgta gctgaattta aaggctctaa gcaccgtaac gacagcccct
     5221 ggtaaatatt tcttcatatt tagaaaatgc actccatata ggcatatcaa ttaaatattt
     5281 tgttgtaata ctcttctata aactttattc cctgcttata catggcgtat ttttctctgt
     5341 aaaattcgct tttattttta tcagcttctg taagaatttc ttctctcatt atgctggtat
     5401 atccgtctct gtaatcagga aatattccac atcctacacc tgcaattatt gctgcaccaa
     5461 tacaagccgc ctcagtttct ttgaacctaa gtacaggagc acctgttaca ttggcaatta
     5521 tttccgtcca tagattgctc ttgctgcccc cgcctagcat cctcagatgt ccagttttac
     5581 aacctttatt tctaaattct tcaattgcat ggttaatttc aaaagcaaca ccttccataa
     5641 ttgccaatgc catatcatat ctgtcatgtt ccagtcccag gcccattagt gatgctttca
     5701 cattcgggtt atagttaggg tatcccgacc cattaaaata tgggtagaaa aaaacatttt
     5761 cagctcgctt cattaagagt gaagctttgt tatcgatatc cctaaaactc tcacttgcaa
     5821 aattatcctt gaaccactcc atagatactc cacctgttgg aacagagacc agtcctcccc
     5881 ataagttgtt tactatatgc ctccctggtg caatatatgt atccaggtca aatacaggct
     5941 gtttgaatgt acccagaaga acccaggcag tacctgtaga cagaaaaagt tcaccttctt
     6001 ctatggcacc tgctccaacg gctgcacagt actgatcatg tcctccatta attactttta
     6061 cagtagttcc aaggcccaac tcttcagccg cacttgcagc taattcccca actgcaagtc
     6121 ctggttccat gacttcagca agtttttttt cattaattcc tgctgcactc aacagctctt
     6181 catcccataa tccacggttg atatctataa ggtgggtcat tgctccatta gtaggatcaa
     6241 tcacatatct gccagtaagt ttgaaattta tgaaatcgag agttgaaagg aacttatatg
     6301 tcccctcaaa taggtctgaa gcattttcct tcagccattt gatctgtatc agatttcccc
     6361 cgggtgaaag cttccagcct gtctttttat aataaaattc accaggatgc aaattcagca
     6421 aatcattctg ttgatcaatt ccgcgtttat ccatccacac tataacattt gatagagggt
     6481 ttccttcttt atctacaggt acaaggcttc ctccctgtga tgaaatagat aaagccagaa
     6541 tattatatct gtcatttaca tcttgtatac attcctttac ggtttcaacc agagaattcc
     6601 accaatcctc agctttctgc tctaccatat ttctaccgga ctttataatc ccataacctc
     6661 tatatgcact tttcagaaca atacctttat cattaataag cattgcttta gttccggttg
     6721 tacctacatc tattcctatt acatacattt ttataccccc tgtgtgagtc gataagctaa
     6781 aatcagtgct tgaacaaaat tatcacaact attatttatt caagttgttt tgtgagtttg
     6841 attttgttca catctaaatc taaaggcttt aagctctgca acgacagcct ctggtaatta
     6901 cttctttgta ttcaataaat gaactcactc ctcatctcat tatacctgcc ttacttctac
     6961 acctttttct tttatctcat taatttcatc ctcattggca tttttattgg taactattac
     7021 atcaacattt tccagtggag agatatgtgc aaagctcgtc cttcccactt ttgtataatc
     7081 tccaacaaca attgtcttat tagcacgggc aatcatttgt ctgttaattt ccgcttcaag
     7141 ataatgatat gtggaaattc catcttcaga atttatccca tcaacggaaa gtataaactt
     7201 atcagcttta tatcttttta cctgattaat aacatcatcc ccataggtaa actggtcccg
     7261 gggttccaaa attcctccca gcagtataat attaaaatct ctcaaattgg cagattcctg
     7321 tgagaagtaa atggcgttgg ttactatcgt aaggttttta gcatttttta gttcttccag
     7381 aacatacagc gtggtggttc cggaactaat cattaacgtg tcatcatcag aaatcataga
     7441 agcaacttca gcagcaattc tccgcttttc ttccgcattt gctttcattc tatctgaaat
     7501 agacatccta taataagccc tataagtact taccgcacca ccatgtacgc gctccagaag
     7561 tcccatgctt tcaagcttgg ccaagtcact ccttattgtt acctctgaaa tctcaaacaa
     7621 tctgcttaat tgatttactt taactttacc ctgtctggtc agtatttcca atattttgtt
     7681 ccttcgttct tcagttccat attctataag tttttcatct tccataacaa taatttacct
     7741 catataacca gcaatattat ttttaacatt ttaatatttt taacatttat ataattaaat
     7801 attttattat ttaattataa ttattacata tataattgca acactgacct cttaacttat
     7861 cttaacttat cttaacttac ttttcactta cagctcaatc caggattttt tctggtccga
     7921 ctcatatgcc ttttccataa catgctggat atatgcaccc tcccaaaatg atggactggc
     7981 ctgcctcttt tcgtatacgc atgaaaggaa attgtaaaga ctgtggacat gtcctcttat
     8041 ccagcctatt gaaaacttgg aacttggaaa atctccaccg ggttttttga agcgctgtac
     8101 acattctatt tttgtatatc cctttaatcc gccataatga gcatcaggta aagtattatc
     8161 ataatactcc agccaattgg gttccataag attgaacctg attgcaccct tgtctccatg
     8221 aatttcgaat cttaattcat cattggtgcc ggtagctatt ttagaggctt caattgtacc
     8281 aattccgccg cttttcatct tcaccatcat aaatgcaaca tcatcagctt caattttaac
     8341 catatttcca cttttatcag gtctttcagg atatattatt ttagtttctg caaataatga
     8401 tgaatattct ccgaggagat agtacataac atcaagtaca tgagacccca gatcgaatag
     8461 tactccgcct cctccaaact tcttttcctg tttccatcca atgggcttct tcgagtctac
     8521 tgaccccgaa tgcaggtaac atgccctgaa agacataatt tttccgattt tcccttcatc
     8581 aataagttcc ttagctctca ttgtggccgg gaaaaaccta tactgcagtg ccatctgggt
     8641 tataacatca tattggtcaa gtacatctgt tatttctttt gtttcttcat agcttacagc
     8701 caaaggcttg tcgcagtata cgtttttacc tgactttaat gcttttagaa cagcttcttt
     8761 gtgatatatg ttaggagtac atatatcaac tacatttata tcctctcttg agaaaatatc
     8821 gtcaggattg ctcgttgcaa attcaaaatc aagactctcc tttgctttct gtgcaacttc
     8881 aggtactgca tcacatactc ctaccagctt tattttaaaa ggcagattac tgtagtataa
     8941 cggaattgtt ttataaccat atgcatgagt tttgcccata aacccaaatc caactatacc
     9001 aactctgatt tcatccattt ttcttccccc ttgttccgtt caggttaaat tattttataa
     9061 agcaaaggga cactcctttc catatattag gtcacaagtt gggagacaca tctccgcaaa
     9121 tgaagaagtg tcgagaagtg tccccgatag aagtgtcccc gataaattcg tattaattta
     9181 atatcccaat tccttctcta cttccccaac tgcctcatca ataatgtcaa gtccctttaa
     9241 tgccagatca tcatccataa tcaatggagg tgacaacctc aatatatgac cggccggtac
     9301 ccatgcaagg cctttcgaaa atgccctctg gtaaaccagt tttcctgctt catcgaaggg
     9361 ttcttttgta cttttgtctt ttacaagttc catagcaagg agacagccct tacctcttac
     9421 atcgccaatg attttatgtt cttccttcat tttgttcatt ctattaagga atacttctcc
     9481 aagatgtgct gatctttcac aaagattttc atcttcaata acttcaatag aagccagggc
     9541 agcagcgcat gccattggat ttccgccata acttgatgat gctgaaattt tctctatgtt
     9601 gtccttatat ttatcactta cgcacattgc tgtaaccggg aagccatttc caaaaccttt
     9661 acctaatgtc gtaatatcag gagttacatt ccagtgttcc atacagaaca ttttacctgt
     9721 tctccccata catgtaagta cctcatctgc aaaaagcagc atgccatatt tatcacataa
     9781 ttgtcttaat tttggcataa aatcatcagg aggaactaca gaaccgcccc aaccctgtat
     9841 cggttccagg acgatagcag caacctgacc ggttgtttca ttctttatca cattttccag
     9901 atgttcaaca cagaaacaat tacattcagg atgcttcatc ttgaaagggc acctatagca
     9961 gttaggcctt ggagccagga aaaaacccgg agctctcata ccctgacttt tgtcaacacc
    10021 ggcacaactt actgctccca ttgttttccc atggaaatct ctccagaagg atacaaactc
    10081 aaatttaccg gtagcagctc ttgcacatct taaacccgct tccactgctg ttgttcccga
    10141 gtcataaagt tgaataccct tcaaatcccc cattgttatg gaagccagct tttcaagaag
    10201 cttcattttt atttcggtag taaaatcgtg acagttcatt aatttatttg catatgtagc
    10261 aactgcttca ctaattttgg gatgacaatg ccccaatcct gttacatata taccagatga
    10321 aaagtcaata aatacattac cgtccacatc agttaaagta cagcccttac ctgaatcgaa
    10381 cactaccgga aacaatctta cctggctgga atatcctttc atatactttg ctgctctgga
    10441 atgcatctcc cttgatttag gtcctggaag ttcagtttta attgctggta atgattttgc
    10501 atctacctct gcccaatttt tatcatacat ttttaactct ttgccactga aaatatagat
    10561 agtggtaaat ccccatctgt ttttatgttt gcttttaact tttactctgc atatttcgaa
    10621 attcctaatg aagatcctgt acttattata cttgcatatc tatatttttt tgcagatttc
    10681 atggcatccc ctttcttttt gtttgttttc atattttctg gataagcgtc tctgctcacg
    10741 cttaagttta tttaccagtt tttggctttc tttatttaat tattattttt tataccataa
    10801 ataactactg ctaatttttt aatactccat taaaatttaa caatacctat attatctaca
    10861 ctcccttacc ctgtagatat gtccctttat cctttaccct taatactctt ttcccaagta
    10921 tctcttacct tatcatatac attgtcaggt atatcaggac ttccaaaaga gcagttgggc
    10981 gcagtattat cacccgctac ttccgggtcg gtacaccagt tatcgtttct atatttttct
    11041 cttatagttt tatctcggaa atcaggtact gcaattggta tatttccatt gcaaattgaa
    11101 cggtatgcaa gaattccagg aatgcccata tcaagagcct ggtaaatatc tatacactct
    11161 tctccatccg aacgtccaag aattctctca aggaaataat gaactgtata gaaatcactc
    11221 cctccatgcc ccaatgtcct ttttgccaac tcagaagctg ctcttggtgc aggactgtag
    11281 ctggtttcat acggtgtcag gttgttccct tctttaaata cattgattct ggatattcct
    11341 tcatgccatc ggtctgtttc catcatacct tttttaccat atatagaata ccagatagat
    11401 cccggttccc tcttcagatt accatgcaaa ctcttggcag ttgcaccgtt attcatctgc
    11461 ataactatca ttcctgatgt tccacccttg tatcccaatg ctgccatgtt ctctacattt
    11521 ggagtttcaa agccaacaac aacgacaggt ctggttccgg ttacagttat gattgggcca
    11581 atgctgtgag tacaatagta agttgaatac atattatttc tccaatgtct tttgtctcca
    11641 taagttattg aaggccatat ggactcacag tcatgtacat attcaccctc gccgtgcata
    11701 aactccccaa tttcgccaag cctgtacaat cttttcatct cctgggttgc agggaaataa
    11761 cagtagtttt cagcatatga atatatgttt ccgctttttt ccactgcttc tacaagcatt
    11821 actgcttctg caagtgtctg gacaggaagc acttcactta acacatgcct tcctgataat
    11881 agaaattttg cagcatacgg agcatgttga ttggcataat tggctaacac tacagcatcc
    11941 atatcatgat taaagaactc attgaagtgg gaatacagtg aaatttttga atcagaatcc
    12001 cttgctatct tctcgcattt tccctgcagg tattctgatt tgtcacaaat agcaaccagt
    12061 tctgcatccg gatgatttgc cagcacgtta atcatcgtca ttccccgtct ggcaccgaat
    12121 acacctactt taattttttt ctgtgaaaca ttcattggag aacctcctca ttactacttt
    12181 ccagttttca gtttccagat ttaatttagt atcagtacat tattagtata cagtatttgt
    12241 ttttatgctt ttgtttcgtt tacatttcgg ttctattata ttgtatttca ttttccttgt
    12301 caatatgttt cacttttttc gaggaatact ttatccttta tttatttttt attaattggc
    12361 ctggattcta tgttaaaaaa ggcacagtat aacaattttc attgcgtatc gcagcggtta
    12421 gctgcaaaca aataagcatg cgctgcgata aattaaggta ctatatattc tcaaaaagtg
    12481 ccatgtaacg cttatactac atagctttga gcaaaaaagt ttgttttttt tgttaacttt
    12541 ttgagaataa tagtataact gtgccaacgc tataccttta tatacaacaa ctttcttttg
    12601 aatttatgtc tttcagttat catgatatgt tcatgataac ttgtgattgc atattttaca
    12661 taagggctat attatacatt atttttttac tgaacaacta tagataacgt acaaaagaaa
    12721 ctacattaca aaagttttgt acgcttatgt tacattaatg agcacgcaaa aatttaattt
    12781 attccatgta gaatcatttt tgcgttatat atacattatt cctcagttac acctatttcc
    12841 agagtaaact ctcttatcac gccaggttct atatattcaa gagtctcatt ttcctcttct
    12901 tcagccagtc ccattgcata attattgcag ggctccaagc ccagaacata atctcctgct
    12961 ttgagatgct tccactccat aaaattccac aattcatttt tattaaattt tatagaaacg
    13021 catgttttta gtttcctatt tagcaacctg catatagcat agccattttc atcggtctcc
    13081 agattgtgat aaaacatttt acttgtgtat tctacagaag gattgtcaaa cctgttataa
    13141 ttaagcaaat cagcttcaga tgtctccacc cccggcttta tttcctttga tgatacaagt
    13201 aattccgtat cttttccaat tagaggataa ccgaagttca tatggtaaat aatcattagg
    13261 ggtaccttct caaacccatt attttcaaca acatcatgga ttataatttt attctcccca
    13321 agcttggatg ttatagttct tgtcatttga agattctctt tatatagctg agcttcattt
    13381 aaaatacctc tgatacccat taccagttca tcattttccc agttataaat tatcccggtt
    13441 ttttcagaag gaacattgga aacttttcca tgaggactga acttcctgtc atgatacatg
    13501 catccctttc cagcctgcat caatccacat gtagtcagca gtcctccgta aaaactttcg
    13561 ccccatgttg ttttatctgt acagaaatat tccggagata ttacaccgga tttgctaatg
    13621 aaccccaggg gaacaccttt aaattcagcc caagcgatat ccatccccct gtcaggcaaa
    13681 accgtataat tcaaacctcc cccggtcctt atatcaactg cagacatgcc ctttgctctt
    13741 ccgtcggcca ttatatatga tcttggacca cagatttgcg ataagctgcc tactctctca
    13801 aatatctgcc ttttgtcaag aacttcatta aaaagtgttg gcactatgta aacgcctgcc
    13861 tttcatatta cattgcatta ggtgtcgcat tgcataagac attgaacatt caaatgctcg
    13921 aattattcaa ctaatcctga acgttctatt ccttccataa attgtcgctg taaaaataaa
    13981 taagcaagca gtaaaggtaa tactgtaaga aacgttccag ccataattac tccctcatta
    14041 taaaaaatag ctgccatcat atcatcaggt tgtaaacttt ttaacgtatc atacattttg
    14101 ggcagcattg taggtaaaaa ccatttctta ctgcttgtta aatatagagt agcttcaaag
    14161 ctatcattcc aatgccatat catggataat actgagctta ctaatattgg agcctttgct
    14221 acgggaaaga ttatgcgcca aaatgtctgt atacatccac atccatctat tagagcggca
    14281 ttctctaagt cttttggaag acctaaaaag aattgtctga atatgaatat atataatccg
    14341 ccgcgtaacc caaatcctaa aaaagatggt acaatcaggg gtaagtacgt atttatccat
    14401 ctaaatttag aatactgaat gtataaagga gttacaagga cttccggtgg gattataatt
    14461 gataataata cgaacataaa taaaaagtct ctccccttaa atttgaacct tgaataaccg
    14521 tatgctatat atgagcatga gatattgtgt ccaattacac atagtacagt cacaaaaatg
    14581 gaatttttta aatacgtaaa atatttaaga gcaccactag ccaccaggta atttgaccaa
    14641 taaatagtct taggtatcca gcggacagtt atatctatta aatcatcagg cgattttaga
    14701 gaagttacaa tcatataaag aaatggaaat ataaatacta gccccagtcc aaataataaa
    14761 atatatatcc ctaaacgata tataaaattg ctccagtatt ttattctttt tattttaata
    14821 aagaagtacg ataatttata tgaacttcct tcccttttca attcctttaa ttttctcata
    14881 aatacacctc ttttaccttt catatgaact atgtataaat agcttcgata aaataaacat
    14941 taatattatc ataataaaaa taaatatgaa atagatccat cccattgctg caccatattc
    15001 cagattgaat tgtttaatac ttacatttac cagataagtt acaatcgggt tatttatatc
    15061 cgtaaatgag tcaactaatg tataaacaac atttaatagt attattggtg atatcatagg
    15121 cagggttatt ttccagaaca tttcccattc tgtcgcagaa tcacagcgtg cagcttcata
    15181 caatgatttt gaaatacttt gcaatcctga aaggaaaagt aatatctgta ctcctgattt
    15241 ccataaaagc aaggttataa gatttagata gttttggatt attgcagaaa aattggagcc
    15301 caggtattgt aaaacttgct gtggtagcag aattattttt gataaatcaa ttacctgttg
    15361 ctgcactccc atatcaatca attcctgcat aataaatccg gatcccagaa caacaggtaa
    15421 aaagaatatc tccctaaaaa atgctctgaa ctttattttt ttattaagaa gtattgcaat
    15481 aaataacgaa aaaacaatta taataggtgt attgaccagt gtgcttttaa caacactaat
    15541 aaacataggc acaaaattaa tatcccaaac aaatgcctta atataatttg ctgcacccac
    15601 ccattccatt ttgaaacctt taatactggt aattttacta aaactaagat gtattgattg
    15661 tactaaaggg tatacaaaaa aaagtaagaa tccaataatc cacggaaaga caaataaata
    15721 cccttgagat actcggagtg ttctacctct tagcgatatt ctgcgattgt tcatctttct
    15781 tcacccctcc tgtctacaac taaataatcc agtgctttta ttttgtgttc gttaatgctg
    15841 gcttcctgtt cgttataatt aacatatacg acactgccat cttcgtaggt tatactgtat
    15901 aaaccctgat ttagcttttt atgttcaatt atgtttttcg accagatatc tcctaatctt
    15961 tcgttgaact ctttataaat atctactgct gttttaaccc agtcttcata acaagaggaa
    16021 aagtttttat tataatccgt ataccgtaga agctcagctt cattatatgt taattcaaaa
    16081 tatggcatac acccatattc cacccatttg agtttttctc ttgggtaatc ataaaataaa
    16141 tttgcagggt tagaagaagt ataagggata agaccatgta ccaccatttg ataaaatggt
    16201 acctgttcac ttgttatgaa ataacctgta tcctctacag gaatattgca aagcctattt
    16261 gcatacggca aaatataagc attacctcca tggacagcag taaatccaag tttattactt
    16321 gactcctcaa tcatcttaca ccaataatca gccgtgttct gcctaattac aggatatttt
    16381 atattatagt cataatacaa tagctgccct accttttcca attgcaaccc attgatcctg
    16441 tatttattaa tgtcaaataa ataatttcta aacttttcaa aggcatttac ggggttataa
    16501 aggaaatatt cctttttccg atcagttaca accatcttat acatttgata ggcaatatca
    16561 tatcttccta caaatgtccc agctcctttt tgcaaattaa agaaattatc ttgaagcaat
    16621 agctgtatat cgttttccga agtatactca accaaacgtt taagacctga actcccgcct
    16681 aatagcctat taggtggtaa atgtgcattt agtatcccat acccgttttt tgtccatccc
    16741 tttaagctaa cctgaattct atcaatccct tgatttttaa aagtatccag tatctcctgt
    16801 gcctgatcaa aggttgtcat agctatatat ctatcaaaca gcaaatttct ttcttttata
    16861 cccataaata actctaatgc cagtggaata tcttcttgaa cactaatatt tcttttgatt
    16921 ccattgcttt ctattaaata attcctatat acgtttgcca ttccactgta gccagcctca
    16981 tcatgattaa gaaaaatata tcttactgaa tgatcagctt tgattatctc tttttcgacc
    17041 ttgcttgcat tctctacatt aggtcttgga tccttaaatg agcgtctata agtaaactca
    17101 ggatatattc tattaagatt tactatatga ccactggggc aaagatttat agctgtatca
    17161 catgctccat cttctataat agcaagatat ccactgtttc cgattatcat tccgaaaacc
    17221 ggtaacattg cctgctcctg ctttttttgt tcatacgcct catagctatc aatatcaact
    17281 ttatccgttc catatacaaa ccatctatat ttatttgcat atatattggg attttcacca
    17341 aaatgcatta tagcaccaca accatccgga taaaatatat aaccgttttc attatcatct
    17401 gctgctccaa aaaaaggcat taattcaata ttcatcaaac cgtatttccc ggtttctttt
    17461 attttttcca ctggtacttt agcatatagc gaattatcat ctatccaaat gtccactgtt
    17521 gcatttatcc ccagtttcgt aaaattataa cttatgctaa taccattttc tatatctttc
    17581 tcactaacct cagattcttc caaaacagag ttacttaatt tactgctttc ttttgattca
    17641 tttttaaaat cattatacgt aaaactaaat aatgattgca tagatgtccg ggtaaaagca
    17701 ttaatatttt ccgtcccatg cttttcctgg ttcgccattg aaaaccaaat atctccgctt
    17761 cttttatcat gcaccataat actatgattt tcataagaat aatataattc caggtattta
    17821 gtttcactta ttattttgta ttctttatat ttctctttat ctattacttg ggacccaaaa
    17881 actgtgcttc gcactcctat aataaaaata atacagagaa ttattgtaat tatcatcgca
    17941 attaccttgg atttcatact attat
//
LOCUS       DAOSDD010000070        17952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1276_length_17952_cov_5.157289, whole genome shotgun sequence.
ACCESSION   DAOSDD010000070 DAOSDD010000000
VERSION     DAOSDD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17952
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1276_length_17952_cov_5.157289"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PK733_08065"
     CDS             complement(<1..1010)
                     /locus_tag="PK733_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ10533.1"
                     /translation="MVKKAAGINISDKNGLRVVEGLGYYKPNEINDLRDVIKDSTIKY
                     GNLIGFKFKDKNGIITGRTYAQFDKDIDYLGTALISLGLKGTHISIISENRYEWGVCY
                     FSVVNGTGVVVPLDKYLPQNEVENLIIRGKVDAVFYSPAYHNMMMTIAKSNNRVKYYI
                     CMESISAEGDSRFLTLPDLIKKGKVLFENGNRSFTNAPIDRSNMSILLFTSGTTNLSK
                     GVMLSHSNIVSNIKAISSVIHAGPGDVHLSLLPLHHTFENTAGLIYMVYRGVCIAYCD
                     GIRYIAQNLKEYNVTLLVAVPAILEAIYRRMREGIKKSGKEKIFNMMVKISDALRTAG
                     ID"
     gene            complement(1159..1791)
                     /locus_tag="PK733_08070"
     CDS             complement(1159..1791)
                     /locus_tag="PK733_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOJ10534.1"
                     /translation="MKNIDFFKNKLKEFGKNAEETIKTMNENRTADQEANYPTELSNY
                     DNHPADTSTQLYTLGLNNALMIHQENLLGEVRNALEKIENGAYGSCELCGKEIDENRL
                     KAIPYTRLCLECEEEKRPDQEIIRNMRPNEELVLDAPMGRKYLNKQEDDEYEGLDILN
                     DLMKYGSSDTPQDMGGYHDYEDFYTNKTDKQGIVDDMDQVSNEEYKKNLE"
     gene            1893..2018
                     /locus_tag="PK733_08075"
     CDS             1893..2018
                     /locus_tag="PK733_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10535.1"
                     /translation="MKKILVRGISESAVEPFNLSGGRAKSVFEQNNYNSFIQIIL"
     gene            2187..2972
                     /locus_tag="PK733_08080"
     CDS             2187..2972
                     /locus_tag="PK733_08080"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOJ10536.1"
                     /translation="MELRADIGVFGGSGFYSFLDKVEEIKLDTPYGSPSDSIAVAVYE
                     GKRIAFLPRHGKNHQYPPHMIPYRANLYAMKKLGVKKIIAPTAAGSLQPYIKPGDFVI
                     SDQFIDRTSGRKDTFFDGPETKHPSAAYPYCPELRKLAIETGKNIGITIHEKGTVVVI
                     QGPRFSTVAESRWFSKMGWEVINMTQYPECYLAKELGICYANIALITDYDAGLEGHDD
                     IKPVTHEEVLKVFDANNEKVKKMLFEMIKKIDLVHCNCNCGEE"
     gene            2974..4005
                     /gene="mtnA"
                     /locus_tag="PK733_08085"
     CDS             2974..4005
                     /gene="mtnA"
                     /locus_tag="PK733_08085"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOJ10537.1"
                     /translation="MRPLEYKDGVLKLIDQTRLPGEVVWVECRTYNDAAKAIKDMIVR
                     GAPAIGVSAAYGTAIGALSIDTDSKDVFFGELKNICDTISKTRPTAVNLFWAIDRVYK
                     KALVNKNKAIDEIKSVIMNEACLMDQEDVETNKRIGRYGKSLIKEESTVLTHCNAGAL
                     ATCDYGTALGVIRAAHESGKKIQVFADETRPYLQGARLTAWELMQDGIPVTLICDNMA
                     GHFMKEGLIDCIIVGADRIAVNGDTANKIGTYSLSVLAKENDIPFYVAAPVSTIDFSI
                     KTGESIPIEERNAEEVTHIKGIPIAPVGVKVRNPAFDVTPNKYITAIITDKGVVYPPF
                     DTNLPELKM"
     gene            complement(4062..4457)
                     /locus_tag="PK733_08090"
     CDS             complement(4062..4457)
                     /locus_tag="PK733_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10538.1"
                     /translation="MEDKTFELMTKLYSEVTSQMETMNTRLADMDTKLADMDTRLSDM
                     DTRLSDMDRKLDTKADKHDIIRLENKMHNDISALYDGYKQTYEKIEILEKKVDGISDK
                     VEKQEVEIKVIKGGKQAKAKYHPDDANNF"
     gene            complement(4576..5655)
                     /gene="aroB"
                     /locus_tag="PK733_08095"
     CDS             complement(4576..5655)
                     /gene="aroB"
                     /locus_tag="PK733_08095"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOJ10539.1"
                     /translation="MNSLKIGLGDRSYPIYITTDYSFIGECLKKAGIDGKLVVITDHN
                     VDKYQSADFMRVLQTSGYSAEKYVLEPGENSKKLGTLESIYRYLIRMKLDRKSALIAF
                     GGGVVGDITGFAASTYLRGIDFIQVPTTLLAQADSSVGGKTGIDFEGAKNIIGSFYQP
                     RLVYINVNSLRTLPVREMRAGLAEVIKHGVIQDRKFFYYIHNNIDKIFNYDEEVLKYM
                     SKINCSIKGNVVEQDEKENDMRAVLNFGHTIGHAIESVSKFTFLHGECVSVGMIGSCK
                     MSQYMGMISENVTTHIAETLKKAGLPVKVNGIDREIVYQTLFYDKKIKKGKLLFILPK
                     DIGEVVQRFVDDEDLIKRVLSEVIE"
     gene            complement(5841..8969)
                     /gene="ileS"
                     /locus_tag="PK733_08100"
     CDS             complement(5841..8969)
                     /gene="ileS"
                     /locus_tag="PK733_08100"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOJ10540.1"
                     /translation="MYKKVSTSLNFVEREKEVLEFWKEHKVFEKSIEKRKEGPTFTFY
                     DGPPTANGKPHIGHILTRVIKDIIPRYKTMKGYKVLRKAGWDTHGLPVELEVEKLLGI
                     DGKPQIEKYGVEPFIQKCKESVWKYESEWRKMSDRVGFWADMDNPYVTYHNTYIESVW
                     WALKQIWDKGLIYKGHKIVPYCPRCGTSLSSHEVAQGYKDVKEPSIYVKFHVKGQPGV
                     FFLAWTTTPWTLPSNTGLTVNPAETYVKAKCGEETYILAEALAESVLEEKYEILDKEK
                     GNELVGMEYEPLFDFALDTLEKKAYFVVADDFVTLTDGTGIVHTAPAFGEDDARVGRK
                     YDLPFVQLVNEQGRFVDSVTPWKDVFVKDADPEIIKNLDGRNLLYKSIDYEHSYPFCW
                     RCDTPLLYYARSTWYIKTTDIKENMLRNNQKINWLPENIRDGRFGNFLESNIDWALSR
                     ERYWGTPLPIWECECGHRHMIGSIAELKEMGENVPDDIELHKPYIDNVFLKCPKCGSN
                     KMRRVSEVIDCWFDSGAMPFAQWHYPFENKELFDENFPADFISEALDQTRGWFYTLHA
                     ISTLLFDEPAYKNCIVLGLVQDKDGQKMSKHKGNVVDPWTVLDKQGADAIRWYFYTGS
                     APWLPSRFYEDAVNEAQRKFMGTLWNTYAFYILYANIDKFDPTKYKLEYDKLSVMDKW
                     VLSRLHSLIKNVDKSLENYRITEAGRAIQEFTDDLSNWYVRRSRERFWGNGMEQDKIN
                     AYMTLYTVLDTLIKVSAPFVPFMAEEIYQNLVGSIDACAPESIHLCNFPKYKEEYINE
                     ELEKNMALVLKIVVLGRAGRNTANIKTRQPIGKMYVKADSQLPDMYMDVVKDELNVKE
                     VVFTDDTKGFTQYKLKPQLKTVGPKYGKLVGKIGKALNEADGFEVVKTFEDGNMYSFD
                     IDGEEVELSEGDVLVEVSQKEGFISTNENNVTVVLDTNLTPELIEEGFVREIISKVQT
                     MRKEAGFEVEDRIKFFYSNNAKIVEIIEKNKELIAEEVLGNQLNEGEGCCGAYSKEWN
                     INGENVEFAVAR"
     misc_binding    complement(9063..9306)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9376..9828)
                     /locus_tag="PK733_08105"
     CDS             complement(9376..9828)
                     /locus_tag="PK733_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOJ10541.1"
                     /translation="MNYTPNDLQNIAFKKTIMGYSEDEVNQVLDRVIEDYNAYIRENL
                     EIKDKLALLNEGIQHYKSIEESLQNALLVAQKTGEEIKKNSYEKAENIIKEAEIKAQR
                     MVNDANQEVMKIKFEYEEMRKKLYLFKSKSESLLLSQLEILKQMFEDD"
     gene            complement(10029..10313)
                     /locus_tag="PK733_08110"
     CDS             complement(10029..10313)
                     /locus_tag="PK733_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOJ10542.1"
                     /translation="MGTIYQAIDYLLVFIQYAIFARVIISWIPIPRDNKFIRLLYQVT
                     EPVLAPLRGIIERSALGKNMMFDFSPVLAFLIIGLLRNIIRSMMFRASIF"
     gene            complement(10329..10811)
                     /locus_tag="PK733_08115"
     CDS             complement(10329..10811)
                     /locus_tag="PK733_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOJ10543.1"
                     /translation="MAKLFDKVLNLVGWELEEEPEDGFEENEESGPEAYKPDTNEERP
                     RFSFSTNASRKQQSKVVNIHSSSQLKVVVIQPHNFEDAQDICNHLKDNKPVIVNLESM
                     EKEPAQRIVDFFCGSVYALDGSIYKVSSSIFLITPYNVDVMGEFKDELKNKGVFPWMK
                     "
     gene            complement(10906..11619)
                     /locus_tag="PK733_08120"
     CDS             complement(10906..11619)
                     /locus_tag="PK733_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ10544.1"
                     /translation="MHGSEEYQSNIEDNINNIKEKVAAAAQRAGRNPDEIRIIAVTKN
                     VEPLRILEAINAGILDLGENRVQELVDKITVINRDINWHMIGHLQKNKVKYIIDKIKM
                     IHSLDNIELAREINVRAEKVQRVIDVLIQVNVAEEETKYGIYRHQLMDFIKEISNYRN
                     IRVKGLMTIAPKVQDPNDVRPVFQELRKIFIDISRKNIDNIDMDFLSMGMSNDFEVAI
                     EEGANIIRIGTSIFGKRTA"
     gene            complement(11685..13061)
                     /locus_tag="PK733_08125"
     CDS             complement(11685..13061)
                     /locus_tag="PK733_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ10545.1"
                     /translation="MDNGRRNKKIHKIVRKTVKKSSGNSNRYKNNENKMESVANPDKS
                     HRNVRRGTFIVVAFLFVYIPSLLNWFYGNNIATGIIYNGKIEDSINVNGLIVRDETLI
                     KSSFAGKYIPVAEEGEKVAAGSVIATVLKETSSGILSQINEINKKIIDAQNDKSKVNT
                     VFSQDILKLDNEIGRKINLLVDEVNSNRMSNIGRIQSEINEVIQRKAVIIGSEQSDDI
                     YIKSLKEQREKLNKQMVQNVEEKKSKFSGIISYTIDGYEEILKTGAVEKITPDIFNKV
                     ISENLTSRSVEQRDTEADKPFAKIIKDMSCMVVLCQDPKDISFLKESSEAYLRINDIN
                     KILKGRLEYISDEQSGKHIAVLQIDKYAGELSAMRRINVDLIKSTYEGLIVPLRSLID
                     INLTDMTAKIVIVKASYASVREVIIEGMNNEYAVIISDNKTKNGISLYDTYVINPQNI
                     QDGQVITQ"
     gene            complement(13082..14377)
                     /locus_tag="PK733_08130"
     CDS             complement(13082..14377)
                     /locus_tag="PK733_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOJ10546.1"
                     /translation="MKYSKGGFNKDFYKQAFNIDESIIDISQGAEKECREIFEEIDIA
                     REFNQLKVIKAMQDNNLSDSHFAGSTGYGYGDRGREVLEQVYADIFKAEDALVRQQIV
                     SGTHALALCLFGNLRPGDELLAASGKPYDTLEEVIGIRESGEGSLKEFGVTYKQVDLL
                     PDGAFNYDDIRASINEKTKMVLVQRSRGYSWRQALKIKDIEDITAFIKNIKKDIIVLV
                     DNCYGEFVEKQEPLEAGADIVAGSLIKNPGGGIAPTGGYVAGKECYVRNAACRLTTPG
                     IGKEVGASLGQNRLFFQGLFFAPHVVAESLKGAVFTAAVMRKLGFEISPCVNDERGDI
                     IQAIKFNNPENLILFCQGIQKGSPVDSYVTPEPWDMPGYDSQVIMAAGGFIQGASIEL
                     SADAPIKPPYIAYMQGGLVYEHVKLGVMNALQLMKEKRK"
     gene            complement(14849..>17952)
                     /locus_tag="PK733_08135"
     CDS             complement(14849..>17952)
                     /locus_tag="PK733_08135"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ10547.1"
                     /translation="TEVAEKYSQTRATERAFELAWTRSRIESGYLGFKADEIELYLKM
                     LPHIIIPGPLRRKWESYVIANIKGQSGLWPFGISGDVPVILLFIDRKEDIEAVYKILK
                     GHEYWRMKGLEVDLVIITGEEDGYLQPLQEMVKEAVAAGHARDLLGKRGGVFVINKSW
                     LSEEDMALFHAAARIILKSDLSAFDEQIEINYKTTYGERLKNNLINTNGTNYKNDEGI
                     QLYQAEETRKLKFFNETGGFANDGSEYVIYLKNRESTPTPWANIISNENFGFIITESG
                     GGYTWAGNSRENKLTPWSNDPVKDTMGEVIYIRDDETGEFWSITPSPVRNKGEYLITH
                     GAGYTSFKYAHQGIDQELVQFAAVNDPVKISVIKLKGKTGQTSQASQTRKLSLFFFVR
                     PILGVSEKITSQYITTEMDSKSGVFTIKNNYNVDFPGRITFIDSSEQKQSFTGDRMEF
                     IGVEGSLEKPEAVSKENLSGTIGAGLDPCAAIQVEVSLNQGEEKELVFLLGQGKSMEQ
                     VLDIIEKYRNVNRAKRELKEVKKAWKERLEVIQVQTPDDSMNIMLNYWLLYQVISCRL
                     LSRAAFYQSGGAFGFRDQLQDVMSLTYALPEFTRWQILLHSSRQFVEGDVQHWWHVEV
                     GKGIRTRYSDDLLWLPYVTADYIMCTGDWSVLDVEMPYIEDDLLPEDQDERYSIPRVS
                     DKRATLYEHCINSISRALNLGEHGIPLMGSGDWNDGMNKVGSGGRGESVWLGWFLCSI
                     LEKFIPICKKAGDEDRAGQYKEISANIIQAIENNAWDGSWYLRAWFDDGKPLGSTENT
                     ECRIDSIAQSWAAISGLGDIERTKEALKAVQNYLINKEEGIIKLFTPPFDKGDLEPGY
                     IKGYVPGVRENGGQYTHAAVWVILAFAMIGDGDKAHQLFHMINPINHSRTQIETARYK
                     TEPYVMAADVYAIPPNTGRGGWTWYTGAAGWMYRVGVEHILGIKKRGDAILIDPCIPS
                     GWKEYLVKYKYENSLYNIEFKNSNGVNKGVQRIYLDGIEIKGNLIQLKKDGKEHKVEV
                     IMG"
BASE COUNT         5105 a         3915 c         2675 g         6257 t
ORIGIN      
        1 atatcaatgc cagcagttct taacgcatcc gagattttaa ccatcatatt aaatattttt
       61 tcttttcctg attttttaat tccttccctc attctccggt aaatagcttc caatatagca
      121 ggaactgcta ccaggagagt aacattatat tccttaaggt tctgtgctat atatcttatt
      181 ccatcacaat aagctataca tacccctcta tacaccatat agattagtcc tgcagtgttt
      241 tcaaatgtgt gatgcaatgg caataatgac aggtgtacat caccaggccc tgcatgaata
      301 acagatgaaa ttgctttgat attactgact atgtttgaat gagagagcat tacacccttt
      361 gataaattcg tagtacctga tgtaaatagc aatatagaca tattacttct gtcaattgga
      421 gcatttgtaa atgaccggtt tccattttca aacaaaacct ttcctttttt tatgagatca
      481 ggtaatgtaa gaaatctgga atctccttca gcagaaatac tctccatgca aatatagtat
      541 ttaactctgt tgttcgactt tgcaatagtc atcatcatat tatgataggc aggactatag
      601 aaaacagcgt caaccttgcc ccttataata aggttttcaa cttcattttg aggaagatat
      661 ttatctaagg gaacaactac tcctgtccca ttaacaactg aaaaatagca tacaccccac
      721 tcatacctgt tctcactaat aattgatata tgtgttcctt tcagtccaag tgaaataaga
      781 gcagtgccaa gataatcaat atctttatca aattgcgcat atgtccgtcc tgtaattata
      841 ccgttcttat ctttaaattt gaaacctatt aaattcccat acttgatagt gctgtccttt
      901 ataacatccc gcaggtcgtt tatctcattg ggtttataat atccaagacc ttcaacaacc
      961 cgtaatccat ttttatccga aatattaatg cctgcagcct ttttcaccat tctcacttct
     1021 cccccgctat ttgcataata aaaagttttc attaatatta tatgataata tattaaatat
     1081 aatgttatca ggtattatca tctggtaata attatactcg cttttgcaga gaaaatcaag
     1141 tactcatttt taatcgtact actccaaatt ctttttatat tcctcattgg atacctgatc
     1201 catatcatcc acaataccct gtttatctgt tttgtttgta taaaaatctt cataatcgtg
     1261 ataaccgccc atatcctgcg gagtgtcgga agaaccgtat ttcataagat cgttaagtat
     1321 atccagacct tcatattcgt catcttcctg cttatttaag tattttcttc ccataggggc
     1381 atcaagcacc aattcctcat ttggacgcat gttccttatt atctcctgat caggtctttt
     1441 ttcttcctca cattctaggc ataaacgtgt atatggaata gccttaagcc tgttttcatc
     1501 aatttccttt ccacataatt cacatgatcc gtatgcccca ttctcaattt tctctaatgc
     1561 attacgcacc tctccaagga gattctcctg atgaatcatt aaagcattat ttaagcccag
     1621 tgtatataac tgcgtgcttg tatctgcagg atgattatca taatttgata gttcagttgg
     1681 atagtttgcc tcctgatcag ctgttctatt ttcattcatg gtctttatgg tttcttcagc
     1741 attcttgcca aattccttta atttattttt aaaaaaatct atatttttca ttgtattatt
     1801 tccctccatt acagtgttca catatagtgt atagtatcca ttttgtataa aattattaat
     1861 gcatacatcc ttaaggggtg catttaattt atttgaagaa aatattggta aggggcataa
     1921 gtgaaagtgc tgtagagcct tttaatctga gtggaggaag agcaaaatca gtgtttgaac
     1981 aaaataatta caattctttt attcagatta ttttgtgagt ttgattttgc ccgtagctga
     2041 atttaaaggc tctaagcact gtaacgacag cccctggtaa atatttcttc aaattcagta
     2101 aatgcacccc atctttcaca tcatccttga tttttactga aattggggta caatatctta
     2161 tatactatga atttgggagg tattttatgg aattaagagc tgacattggc gttttcggtg
     2221 gttcaggttt ttattcattt cttgacaaag ttgaagaaat taaattagat actccatatg
     2281 gtagtcccag tgattctatt gccgtggctg tttacgaggg taaaagaata gcgtttctgc
     2341 cccgtcacgg gaaaaatcat cagtatcccc ctcacatgat accatataga gcaaatttat
     2401 acgcaatgaa gaagcttggc gtaaagaaaa taattgcgcc tactgccgca ggaagtttgc
     2461 aaccctatat aaaaccgggg gatttcgtga tttccgacca gtttattgac aggacaagcg
     2521 gccgtaaaga cacctttttt gacggtccgg aaacaaagca tccaagtgct gcataccctt
     2581 attgcccgga gctcagaaaa cttgcaattg aaacaggaaa gaatatcggt attactattc
     2641 atgaaaaagg aactgttgtt gtaattcaag gtccaagatt ttctactgtt gcagaaagcc
     2701 gctggttcag caaaatgggg tgggaagtaa taaacatgac ccaataccct gaatgctatc
     2761 tggctaaaga acttggaata tgttatgcga atattgcatt aataactgat tatgatgcag
     2821 gtctggaagg acatgatgac attaaacctg ttacccatga agaggtttta aaagtttttg
     2881 atgcaaacaa tgaaaaagtt aaaaaaatgt tatttgaaat gattaaaaag atagatttgg
     2941 tgcattgcaa ttgcaattgt ggggaggaat agcatgcgac cattggaata taaggacgga
     3001 gtcctaaaac tgattgatca gacaaggctt ccgggggaag ttgtctgggt agaatgcaga
     3061 acttataatg atgctgcaaa agctatcaaa gatatgattg tcaggggagc acctgctatt
     3121 ggggtatcag cagcctatgg gacagctata ggcgccctgt ctatagatac agattcaaag
     3181 gatgttttct ttggtgagct taaaaatata tgtgatacca taagtaaaac ccgtcctact
     3241 gcagtaaact tattctgggc gattgataga gtatataaaa aagctcttgt aaataaaaat
     3301 aaagcaattg atgaaataaa aagtgttatt atgaatgaag cctgcctaat ggatcaggag
     3361 gatgttgaaa caaataagag aattggccgt tatggaaaat cgttaattaa agaagagtca
     3421 acagttctta cacattgcaa tgcaggtgct ctggcaacat gtgactatgg aacagccctt
     3481 ggcgttatta gagcagcaca tgaatcaggt aaaaaaatac aggtgttcgc tgatgaaacc
     3541 aggccctatc ttcaaggtgc cagactgacg gcttgggaac ttatgcagga tggcatacct
     3601 gtcaccctca tatgcgataa tatggcaggt catttcatga aagaaggctt aattgattgc
     3661 ataattgtag gtgcagatcg tattgcagtt aatggagata cggcaaacaa aattggcacc
     3721 tattctcttt ccgttcttgc taaagaaaat gatatacctt tttatgtggc agcgcctgta
     3781 tcaacaattg atttttcaat aaaaacaggg gaaagcattc ccatagaaga aagaaacgct
     3841 gaggaagtaa cccatataaa aggaattcct attgcacctg taggagttaa ggtaagaaac
     3901 ccggcttttg atgttacacc caataagtat ataactgcaa taataacaga taaaggagta
     3961 gtttatccgc cctttgatac taatttaccg gaacttaaga tgtaatatga ttagatatta
     4021 agtaaacaga attaatgtta tatgccactc ttgatggttc tttagaaatt atttgcatca
     4081 tcagggtggt atttagcttt agcttgcttt ccgcctttaa ttaccttaat ctcgacctcc
     4141 tgtttttcaa ctttatcaga aataccatct accttttttt ctagtatttc aattttttca
     4201 tatgtctgtt tataaccatc atataaagcg ctaatatcat tatgcatttt attttccagt
     4261 cttattatat catgcttgtc tgctttagta tccagtttcc tatccatgtc actcagcctg
     4321 gtatccatgt cactcagtct ggtatccatg tcagccagct tggtatccat gtcagccaac
     4381 ctggtgttca ttgtttccat ttgtgaagtt acttcactat ataatttggt cattaattca
     4441 aatgttttat cttccatgac agagacctcc tttgttttct ttattttacc atatatttta
     4501 agagcagtca acagtatttt ggtatttatt acattaattg tttatatttg ttagcatcat
     4561 gactatatta tatgattatt caattacttc tgatagaact ctttttataa gatcttcatc
     4621 atctacaaaa cgctgaacaa cttcaccgat atcttttggc agaataaata agagcttgcc
     4681 ttttttaatt tttttatcat aaaatagtgt ctgataaact atttctctgt caataccatt
     4741 tacctttaca ggcagccctg ctttttttaa agtttctgca atatgggtcg tcacgttttc
     4801 actaatcata cccatgtact gtgacatttt gcaggatcct atcattccta ctgatacaca
     4861 ctctccatgc aggaacgtga atttggaaac actttctata gcatgcccta tagtatgtcc
     4921 gaaattcagt accgctctca tatcgttttc cttttcatcc tgctcaacta cattcccttt
     4981 aattgaacag ttaattttac tcatatattt aagtacttct tcatcataat tgaatatttt
     5041 atcgatgtta ttatggatat aataaaaaaa ctttctgtcc tgaataactc catgttttat
     5101 tacctcagcc aatccagccc tcatttctct tacaggaagt gtccttaaag aatttacatt
     5161 gatataaacc agtctgggtt gataaaaaga tccaataata ttctttgctc cctcaaaatc
     5221 tattccagtc tttcccccta cgctgctatc tgcttgagcc agcaaggtag tcggcacctg
     5281 aatgaagtca atacccctta ggtatgtaga agcggcaaat cctgtaatat cacctacaac
     5341 tccgccgcca aaagctataa gtgcagattt acgatccagc ttcattctga ttaaatatct
     5401 gtatatactt tctaaagttc caagtttttt gctgttttct cctggctcaa ggacatactt
     5461 ctcagctgaa taacctgaag tctgcaatac tcgcataaag tctgcagact gatatttatc
     5521 aacattatga tctgttatta caaccaattt accgtcaatt cctgcttttt taagacattc
     5581 tccaatgaag ctgtaatctg tagttatata tatgggataa ctcctatccc ccagtcctat
     5641 ttttaaactg ttcaaaacca acactcctaa cttgtcactt gccgcttacc actagccact
     5701 tctaatctct ataagtctac tcaaaaaagc ggactcgtgc agtccgcttt gatagctcag
     5761 actttccgag cagactccca gcctctagct tctaacttcc aacttctcac ctcttacttc
     5821 caatttctca cttccaatat ttatctcgct acagcaaact caacattttc tccgtttata
     5881 ttccactctt tgctgtatgc tccgcaacat ccctcacctt catttaattg gtttccaagg
     5941 acctcctctg caataagttc cttatttttc tcaattattt caacaatttt tgcattattg
     6001 ctgtagaaga attttattct gtcttccact tcaaagcctg cttcctttct catggtctga
     6061 accttgctga taatctctct tacaaatcct tcctcaatta gttcaggagt cagatttgta
     6121 tccaatacta cagtaacatt gttctcgttt gtagaaataa atccttcctt ttgacttact
     6181 tcaacaagaa catcaccctc agatagttca acttcttctc catcaatatc aaatgaatac
     6241 atattgccat cttcaaatgt tttcactact tcaaagccat cagcttcatt taaggccttc
     6301 ccgatctttc ccactagttt tccatatttg ggtccaactg ttttcaattg gggtttaagc
     6361 ttgtattgcg taaatccctt agtatcatca gtgaaaacta cttcctttac attcagttca
     6421 tctttaacaa catccatata catatccggt agctgggaat cagcttttac atacatttta
     6481 ccaataggct gccttgtttt tatattagcg gtattccttc ctgcacgacc aaggacaaca
     6541 atcttcaaaa caagagccat attcttttcg agttcttcat taatatactc ttccttgtat
     6601 ttagggaaat tacacaaatg aatactttcc ggtgcacatg catcaatact tccaaccagg
     6661 ttctgataaa tttcctcagc cataaacgga acaaatggtg ctgatacctt tattaatgta
     6721 tcaagtactg tataaagagt catataggca tttattttgt cctgttccat tccattgccc
     6781 cagaaacgtt ctctgcttct tcttacatac caattgctta aatcatcagt aaattcctgg
     6841 atagctctcc ctgcttcagt aatacggtaa ttttccaagc ttttgtcaac attttttatc
     6901 agtgaatgca atcttgacaa aacccattta tccataactg atagcttgtc atattccagc
     6961 ttatattttg ttgggtcgaa tttatcaata ttggcgtaca gtatatagaa tgcataggta
     7021 ttccataatg tacccatgaa ttttctctgc gcttcgttta ctgcatcttc ataaaagcgg
     7081 ctgggaagcc agggggcact tccagtataa aaataccagc gtattgcatc tgcaccctgt
     7141 ttgtcgagta cagtccatgg gtcaacaaca ttacccttat gcttgctcat tttctgtccg
     7201 tccttatcct gaaccaatcc tagtacaata cagttcttat atgcaggctc atcaaagagg
     7261 agtgttgata tagcatgcag agtatagaac catcccctgg tttggtcaag agcttcactt
     7321 ataaagtccg cagggaaatt ttcatcaaac agctctttgt tttcaaaagg ataatgccat
     7381 tgggcaaatg gcattgcacc agaatcaaac cagcagtcaa ttacttcgga aactcttctc
     7441 attttattgg aaccgcattt tgggcactta aggaaaacat tatctatata tggcttgtga
     7501 agttcaatat catctggaac attttcgccc atttccttca attctgctat gctgccaatc
     7561 atgtgcctgt gtccgcattc acattcccat atgggaaggg gtgtgcccca atacctttca
     7621 cggcttaaag cccaatcaat attgctttca aggaaattgc caaatctgcc gtctctgata
     7681 ttttctggga gccaattaat cttttgatta ttccggagca tattttcctt aatatcagtt
     7741 gtctttatat accaggtact ccttgcataa tagagtaaag gtgtatcaca tctccagcaa
     7801 aaaggatatg agtgttcata atcaatggat ttataaagca ggtttctccc atccagattc
     7861 tttattatct ccgggtctgc atcttttaca aacacatctt tccatggggt tacagaatca
     7921 acaaacctgc cctgctcatt aacaagttga acaaatggaa ggtcatattt ccggcctact
     7981 ctggcatcat cttcaccaaa agcaggtgca gtatgaacaa taccggtacc atccgtaagc
     8041 gtaacgaaat catcagctac tacaaagtat gccttttttt ccagggtatc cagtgcaaaa
     8101 tcaaacaatg gttcatactc catcccgaca agctcattgc ctttttcctt atctagtatt
     8161 tcatatttct cttccaatac actttctgca agagcttctg ccagaatata ggtttcttct
     8221 ccacattttg ctttaacata ggtttctgca ggatttacag taagacctgt atttgaaggc
     8281 aatgtccatg gggttgtagt ccatgccagg aaaaaaactc ccggctgccc ttttacatgg
     8341 aattttacat aaatagatgg ttctttaaca tccttatagc cctgggcaac ttcgtgactt
     8401 gaaagggaag ttccacagcg gggacaataa ggaacaattt tatgcccttt atatataagg
     8461 cccttatccc atatttgctt taatgcccac catacagact caatataggt attatgataa
     8521 gtcacatatg ggttatccat atctgcccag aagcctactc tgtcactcat ctttctccat
     8581 tcactctcat atttccatac actttcctta cacttctgaa tgaacggttc tacaccatat
     8641 ttttctattt ggggtttacc gtctattcca agcagctttt caacctccag ctctacaggc
     8701 agtccatgag tatcccatcc tgcttttcta agaaccttat aacctttcat tgtcttgtat
     8761 cttggtatta tatccttaat tactcttgtt agtatatgtc caatatgagg ctttccattt
     8821 gctgttggcg gtccatcgta aaaagtgaag gtaggccctt ctttcctctt ttcgatactc
     8881 ttctcaaata ctttatgttc cttccagaac tccaggactt ctttttctct ttctacaaag
     8941 tttaagcttg ttgatacttt cttatacaac ttataaatcc tccttcattt agataattta
     9001 tattttcata tgcaatagat acattgctat tttaataaaa aatcaaaaac cctctcatcc
     9061 tgcataggga cgagagggca ttatctcacg cggtaccacc caaattatta tgactatggt
     9121 atcataatca cttctttttt taacggtttt tgccggctta ccttactttc acatacatta
     9181 caaatgtaat aatttcagtc agcaactcat ggatgatttt caaagacaag ccttacaggg
     9241 tttccaccag cacctgctct ctttaaaggt ttactgcctt ttactctttc cagtcatagt
     9301 ttttagctaa cattaaatta tgaaaattat atatcatcag gtatacagct gtcaaggggc
     9361 agattattaa gccttttaat catcctcaaa catttgcttt aaaatctcaa gctgtgacaa
     9421 taaaagcgat tctgatttcg acttaaataa atacagcttc tttcgcattt cctcgtactc
     9481 aaacttgatt ttcattactt cctggttagc atcatttacc attctctgag ctttaatttc
     9541 agcttcctta atgatatttt cagctttttc ataggaattc ttcttaattt cctctcctgt
     9601 tttttgagca acaagaagag cattctgcaa tgattcctca atgcttttat aatgttgaat
     9661 accttcattc agtaatgcaa gcttgtcttt tatttccagg ttttccctta tgtaagcatt
     9721 ataatcttct attaccctgt ccagtacctg attaacttcg tcttcactat aacccattat
     9781 agtcttttta aatgctatgt tttgcaaatc attgggagta taattcatac taagctccat
     9841 tcctgctacg tgcgaaatga attcgccctt gcaggaacaa aacttttttt gattaaaaaa
     9901 gtttttatgg gcttaaattc attttagccg cgcgtgcaat ttattttttt taaaattctt
     9961 gtgcaaaaaa tttgcacatg cgcattagtt tttcagaaat ttattgattt ctgaaaaact
    10021 ttatcacctc aaaaaataga tgcacggaac atcatgcttc taataatatt tctcaataaa
    10081 cctattatta aaaaagccaa gaccggagaa aaatcaaaca tcatgttttt tccaagagcg
    10141 gatctttcaa ttattcccct taacggcgcc aaaacaggct cggtaacctg atacaaaagc
    10201 cggataaatt tgttatccct cggtataggt atccatgata ttattaccct tgcaaaaatt
    10261 gcatattgta taaatactaa aagataatct attgcctgat aaattgttcc cactattatt
    10321 atcctccatt atttcatcca gggaaataca cctttatttt taagctcgtc cttaaattct
    10381 cccattacat caacattata tggtgtaatt aaaaatattg aactggaaac cttataaata
    10441 ctgccatcaa gtgcgtatac agaaccacag aaaaaatcta ctattctttg agcaggttct
    10501 ttttccattg attccaaatt cacaataaca ggtttgttat cttttaaatg attgcatata
    10561 tcttgcgcat cctcaaaatt gtgtggttga ataacaacta ccttaagttg ggaagatgaa
    10621 tggatattaa ctactttact ctgctgtttt ctgctggcat ttgtagaaaa ggaaaatctt
    10681 ggccgttctt catttgtatc aggtttatat gcttccggac cactttcttc attttcctca
    10741 aatccatctt caggctcttc ttccaactcc catccaacaa gatttaatac cttatcaaaa
    10801 agttttgcca taatatactt ctcccttctt ttcattcgta aaaagcaagc ttagctcatt
    10861 taaggcagtc tatacttttc tatgtgataa aaaatgctga ttgtttcacg ctgtcctctt
    10921 tccaaatatg gatgtaccta ttcttattat atttgcacct tcttcaattg cgacctcgaa
    10981 atcattgctc atccccatag acaggaaatc catatctata ttatcaatgt ttttcctgct
    11041 tatgtcaata aatattttcc tgagttcttg aaaaacaggc cttacatcat taggatcttg
    11101 aaccttaggc gctatggtca tgagtccttt aaccctaata tttctgtaat tgcttatttc
    11161 tttaataaaa tccataagct ggtgtctgta tatcccatat tttgtttctt cttcagctac
    11221 attaacctga atcaacacat caataaccct ttgaactttt tcagccctta catttatttc
    11281 ccgggcaagc tcaatattat ccaaagaatg tatcatcttt atcttatcaa ttatgtattt
    11341 tactttattt ttttgaagat gaccaatcat atgccagtta atatctctgt taataactgt
    11401 aattttatca accaattctt gcactctatt ttcacccagg tccaaaatac cagcattaat
    11461 agcttcaagt atcctcaaag gctctacatt cttcgttact gcaataattc taatttcatc
    11521 agggtttcgc cccgctcttt gtgctgctgc tgcaactttt tctttaatat tattaatatt
    11581 atcttcaatg ttgctctgat attcttcaga gccatggatt cccttataga tttgagattc
    11641 ttcatagttc tgagtttctt tatatttcct aatatcttca tcgttcattg tgttatcacc
    11701 tgaccatcct ggatattttg ggggtttatt acataagtat cataaagcga tatcccattt
    11761 ttagtcttgt tatcgcttat tataacagca tattcattgt tcataccctc aattataact
    11821 tcccttacac tggcgtaact tgcttttaca ataactattt ttgcagtcat atcagtcaga
    11881 ttaatatcaa tcaggctcct taaaggcact atcaggcctt catatgtaga ttttattaaa
    11941 tcaacattaa ttctcctcat tgctgacaat tcacctgcat acttgtcgat ttgcaaaaca
    12001 gctatatgtt tacccgactg ttcatcagaa atatattcta atcttccttt caaaattttg
    12061 ttgatatcat taatcctcaa atatgcttca ctactttcct tcaaaaaact tatatctttc
    12121 ggatcctggc atagaacaac catacagctc atatctttaa taattttagc gaagggttta
    12181 tctgcttctg tatctctctg ttctacggac ctgctcgtca aattttcaga aattacttta
    12241 ttaaatatgt cgggagttat cttttccaca gcaccagtct tcaggatttc ctcatatcca
    12301 tcgattgtat aagatataat acctgaaaat ttagattttt tttcctctac attttgtacc
    12361 atttgcttat taagtttttc tctctgttcc tttaaagatt ttatataaat atcatcactc
    12421 tgttcgctcc caattattac agcttttctc tgaataactt cattaatttc cgattgtatt
    12481 ctaccaatat tgctcattct attgctattt acttcatcga ccagtaaatt tatttttcgc
    12541 ccaatttcat tgtccagttt caaaatatcc tggctgaata ctgtatttac tttgctttta
    12601 tcattttgtg catcaataat ttttttattt atttcgttta tctggctaag gataccagag
    12661 gatgtttctt tcaaaactgt ggcaataact gagccggcag ctactttttc cccttcctct
    12721 gctacaggta tatatttccc agcaaaagaa gactttatta atgtctcatc tctaacaatt
    12781 agtccattta cattaattga atcttcaatt ttgccattat atattatacc ggttgctata
    12841 ttatttccat aaaaccagtt aagtaatgaa gggatatata caaacagaaa agcaactaca
    12901 ataaaagttc cccttctcac atttctatgg cttttatcag gatttgcaac actttccatt
    12961 ttattttcat tatttttgta cctatttgaa ttaccactcg atttttttac agtctttctg
    13021 acaattttat gtattttttt atttcgtctt ccgttatcca tatttccctc acctgatcat
    13081 actatttcct cttttccttc atcaactgca aagcattcat aactcccagc ttaacatgtt
    13141 catatactaa tccgccctgc atataggcaa tatagggtgg ctttatgggt gcatcagcac
    13201 ttaattctat tgacgctccc tgaataaatc cgcctgcagc cattataact tgactgtcat
    13261 agcccggcat gtcccatggt tcaggtgtaa cataagaatc cactggagac cctttttgta
    13321 ttccctgaca gaaaagtatt aggttttccg ggttattaaa cttgattgcc tgaattatat
    13381 cgccacgttc atcatttaca cagggagaaa tttcaaatcc aagttttctc attacagcag
    13441 cagtaaaaac agcgcctttc aaactttcgg caactacatg gggcgcaaaa aacaggcctt
    13501 gaaagaacaa tctgttttgc ccaagagatg cccctacttc cttgccaata ccaggcgtag
    13561 tcaatctgca tgcagcattt cttacataac attcctttcc tgctacatat cctccagtag
    13621 gtgctattcc ccctcccgga tttttaataa gtgaacctgc aactatatca gctcctgcct
    13681 ccaaaggctc ctgtttttct acaaattcac catagcaatt gtccacaaga actattatat
    13741 cctttttgat gttcttaata aaagctgtaa tatcctcaat atctttaatt tttaaagcct
    13801 gcctccagct atatcctctt gaacgctgta ccaataccat ttttgttttt tcatttattg
    13861 atgctcggat atcatcataa ttaaaagccc catcaggtaa taaatccacc tgcttatatg
    13921 taacaccaaa ttcttttagt gaaccttctc cgctctccct gattcctata acctcttcaa
    13981 gagtatcata gggcttcccg gaagctgcca gcaattcatc tcccggtctt aaatttccaa
    14041 aaaggcaaag agccagagca tgagttcctg aaacaatttg ctgccttact aatgcgtctt
    14101 ccgccttgaa aatatctgca tatacctgct ctaatacctc acgccccctg tcgccataac
    14161 catatcccgt tgaacctgca aaatgcgaat cactcaagtt gttatcctgc atggctttaa
    14221 tcactttcaa ctggttgaac tctcttgcga tgtcaatctc ttcaaaaatt tccctgcact
    14281 ctttttcagc accctgcgaa atatcaataa tgctttcatc tatattaaaa gcctgtttgt
    14341 aaaagtcttt attaaatcca cctttgctgt acttcattat gctgcctcca tgataagtta
    14401 atttcttttc attattgtat tatatcatga atttcgaaga aattgcatga tataattgtg
    14461 tatgtgcgtt ttttgcaaag caaaaatatt cgcacgcaca taaattccgc cggaggcata
    14521 taatatccga atttatgagg atattatatc atgaatttcg aagaaattgc atgatataat
    14581 tgtgtatgtg cgttttttgc aaagcaaaaa tattcgcacg cacataaatt ccgccggagg
    14641 catataatat ccgaatttat gaggatatta tatcatgaat ttcgaagaaa ttgcatgata
    14701 taattgtgca tgtgcgtttt ttgcaaagca aaaatattcg cacgcacata aattccgccg
    14761 gaggcatata atatccgaat ttatgaggat attatatcat agccaggctt acagctgcaa
    14821 taaggtaaaa tctccattga atccagggct accccataat aacctcgacc ttatgctcct
    14881 ttccatcctt tttcaactgg atcaagtttc cctttatctc tatcccatct aaatatattc
    14941 tctggactcc tttatttact ccattggagt ttttaaattc tatgttgtac aatgagtttt
    15001 catatttata ctttaccaaa tattctttcc atcctgaagg aatacatgga tcaataagta
    15061 ttgcatctcc ccgcttctta atacccagaa tatgctcaac tcctacgcgg tacatccatc
    15121 cggctgctcc tgtataccat gtccatcctc ctctgcctgt attgggcggt atagcatata
    15181 catcagctgc cataacataa ggctctgtct tatacctggc tgtttcaatc tgagtacgtg
    15241 aatggtttat agggtttatc atatgaaata actgatgagc tttatcccca tctcctatca
    15301 ttgcgaaggc aagtataacc catactgctg catgggtgta ttgtcctccg ttttctctta
    15361 ctcccggcac atagcctttt atataaccag gctccagatc ccccttatcg aaaggaggtg
    15421 tgaaaagctt tataattcct tcttccttgt taattaaata attctgtact gcttttaatg
    15481 cttcctttgt gcgctctata tcacccagtc cggatatagc agcccaagac tgagctattg
    15541 agtctattct gcattccgtg ttctcagtgg aacccagcgg ttttccatcg tcaaaccatg
    15601 ccctcagata ccagcttcca tcccaggcat tattttcgat tgcttgaata atattagctg
    15661 aaatctcttt atattgccct gctctatctt catcccccgc ctttttgcaa atagggataa
    15721 atttttcaag tatgctgcac aagaaccacc ccagccatac actttctccc ctgcctccgc
    15781 tgcctacttt attcattccg tcgttccagt ctcctgaacc catcagagga ataccatgct
    15841 cacctaaatt taacgctctt gaaatagaat ttatgcaatg ctcatataag gttgcccttt
    15901 tatctgaaac acgtggtata ctataccttt catcctgatc ttcaggcagc aggtcatctt
    15961 caatataagg catttctaca tcaagtacgc tccagtctcc tgtacacatt atataatctg
    16021 ctgttacata tggcagccac aaaagatcat ctgaatatct ggtgcgtatt ccttttccca
    16081 cttcaacatg ccaccagtgc tgtacatcgc cctcaacaaa ttgtctggat gaatgcagaa
    16141 gtatctgcca tcttgtgaat tcaggcaaag cataggtcag agacattaca tcctgcaact
    16201 ggtctctaaa accaaaagcc ccacctgact gatagaaagc agctctgctc aaaaggcggc
    16261 aggatataac ctgatatagc agccaataat tcaacataat gttcatagaa tcatcaggag
    16321 tttgaacctg aataacttca agtctttcct tccatgcttt tttaacctcc tttaattccc
    16381 ttttggctct atttacattc ctatattttt ctattatatc cagcacctgt tccatacttt
    16441 ttccctgtcc cagaagaaat accagctctt tttcctctcc ctggttaagg ctgacttcaa
    16501 cctgtattgc tgcacaagga tctaatcctg cacctatggt acctgatagg ttttccttcg
    16561 aaaccgcctc tggtttctca aggcttccct caactcctat aaattccatt ctatcccctg
    16621 taaagctttg tttctgctca gaagaatcta taaaagttat tcttccggga aaatcaacat
    16681 tataattgtt tttgatggta aaaacacctg atttactatc catctccgtt gttatatatt
    16741 gtgaagtgat tttttcactt actcccaaaa taggtcttac aaagaagaac agggagagct
    16801 tccttgtctg acttgcctgg cttgtctggc ctgtttttcc ctttaattta ataactgaaa
    16861 ttttaacagg atcatttact gctgcaaact ggacaagttc ctgatcgatg ccctgatgtg
    16921 catacttaaa agaagtataa ccggctccat gggttattaa atattctcct ttatttctta
    16981 caggggaagg tgttatactc caaaactctc ctgtttcatc atcccttata taaattactt
    17041 ctcccatcgt gtcctttaca gggtcatttg accatggagt aagcttattc tctctgctgt
    17101 ttccagccca ggtatagcca cctcctgatt ctgtaattat gaaaccgaaa ttttcgtttg
    17161 atataatatt agcccagggt gtaggagtgc tttctctatt ctttaaatag atgacatact
    17221 cacttccgtc attcgcaaac cctcctgttt cattaaagaa ttttaatttt ctggtttctt
    17281 cagcttgata tagctgtatc ccctcgtcat ttttgtaatt agtaccgttt gtatttatga
    17341 gattgttttt taacctctct ccatacgttg ttttataatt tatttcaatc tgctcatcaa
    17401 aggcgcttaa gtcacttttt aatattatac gggctgctgc atgaaacaag gccatatctt
    17461 cttctgataa ccaggactta tttataacaa atactccacc ccttttaccc agcaagtctc
    17521 ttgcatgtcc tgctgccacc gcttctttaa ccatttcctg cagcggctgg agatacccgt
    17581 cttcctcgcc tgttattatt accagatcaa cttccagtcc tttcatcctc caatattcat
    17641 ggcctttcag gatcttgtaa acagcttcaa tatcttcttt tctatcaata aatagaagta
    17701 ttacaggtac atcacctgat ataccaaatg gccacagccc ggactggcct tttatatttg
    17761 caattacata gctttcccat tttcttctta agggtccggg tattataata tgaggaagca
    17821 tcttgagata tagttctatt tcatctgctt taaatcccag ataaccggat tctatacggc
    17881 ttcttgtcca ggcaagttca aaagctcttt cagtagctct ggtctgactg tatttttcag
    17941 caacctcagt ga
//
LOCUS       DAOSDD010000071        17877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1281_length_17877_cov_5.424812, whole genome shotgun sequence.
ACCESSION   DAOSDD010000071 DAOSDD010000000
VERSION     DAOSDD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17877
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_17877_cov_5.424812"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1592)
                     /locus_tag="PK733_08140"
     CDS             complement(57..1592)
                     /locus_tag="PK733_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10548.1"
                     /translation="MYNFTNKQYVFKQSSGKIWNIYYDKQLGICYSTLTKRNTWTAPA
                     SLEKKAHESFSACMDSKDKFHIIYQSIHGDIYYIRLDESSVVTLPVLNSRYPSLYNKY
                     FEIIPIKNTIHFFYVLQHKNNLLLSHQILSDKEVQNPQAVGYISMMNLPYTAVADKSG
                     NIYTFYQPAVTGALHKNSSLQLGYSRFSTHHNLREEFISISTPGINTDYPSTAIDNSG
                     LIHFSYQNLVSGRYDLVYRQKAPNSMEWSSEAIIHSSSNPFDNSTIICIDDKVVVYWV
                     KNDVIYYCYSSDKGNSWSKSGRYNFPVGRQLACISYGTNDFYEVNRISAGDIPGSFIN
                     GLKMAFYQDLLNTDNTNTDKEGLRNLISDNIKSLSENIEDLSESNNSLNAEIQRINLF
                     HKNILKELDKYSIRISFLENELKQIKSMAQHTFVSHNKYNIRSVVRNEANETNSTNEA
                     NKANKVNEINEINEINEVDVVDEVDEIDEIDEIDEVKGINEINRPNENNDSDRINSFD
                     ENS"
     gene            1763..2692
                     /locus_tag="PK733_08145"
     CDS             1763..2692
                     /locus_tag="PK733_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10549.1"
                     /translation="MSFDNDNDRVVSGLLCDKDIAKVREAVCIQVEKVYDSCKEKDCI
                     EDVKVLFKHYHKVQKIINNSINVKCKKAEVIDVYADVEPVPFKRGFFTVDVKFFIKVT
                     LDFFVSRLRGGTKICTIPGIVLFDKKVILFGSEGNIKIFKSHFTDPCYDVPIKSALQQ
                     DNQPIAKVEVAEPIALNAKIQDLMDKCFEDTCCFDQFPRGVAEALDADNFDDSDIVEI
                     AGEDERCYPTRRVVVTIGLFSIIKLVRDVQLLIPAFDFCVPKKKCIASTEENPCELFE
                     TIEFPVDEFFPPQKFDFPRALEEEAVMQRKAEE"
     gene            complement(2934..3308)
                     /locus_tag="PK733_08150"
     CDS             complement(2934..3308)
                     /locus_tag="PK733_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOJ10550.1"
                     /translation="MDNQYENTQAQTGTFNITHFIIRLIVGAVVLAITAALTPGFTIS
                     GIWPLIFGAIVLAILDYLVLRVLGVNATPFGRGLTGFILAAVIIYVTQFFVAGFNVTV
                     WGAIIGALVYGLVDLIIPGRAM"
     gene            3465..4391
                     /locus_tag="PK733_08155"
     CDS             3465..4391
                     /locus_tag="PK733_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOJ10551.1"
                     /translation="MKLNLWLNNEMPDIAKSLEGINNIYYFNQKTIGLSGKEKIYKII
                     HDVRSALFPGVYEKCPIDETRINIFIGNNIREAAIELSGLIEKTLIDICDTEDKETRN
                     CTKCRSRANDITIHLMKKLPEIREKLHMDIQAAYTGDPAALTTEEILLSYPSIDAISI
                     YRIANILYEQKVPIIPRVMSEYAHQRTGIDIHPGAKIGKYFFIDHGTGVVIGETCTIG
                     NNVKIYQGVTLGAKSFQLDEHGNPVKGVKRHPDIEDNVVIYAGATILGGDTVIGHDSI
                     IGGNVWLTHSVPPYSKVYNAQPSPIIKQGKPE"
     gene            4611..6020
                     /gene="cysS"
                     /locus_tag="PK733_08160"
     CDS             4611..6020
                     /gene="cysS"
                     /locus_tag="PK733_08160"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOJ10552.1"
                     /translation="MKVYNTLTRKKEEFESIQSGKVRIYSCGPTVYNYAHVGNLRTYV
                     FMDILRRVLKYEGYEFKHVMNITDVGHLVSDADEGEDKLVKTAKEQKKSPWEIADYYT
                     RIFFEDIDSLNIERPEIIAKATEHIKEMLEFVEGLVEMGYGYETSDGIYFDIGKFEGY
                     GKLSGINLEEQLAGARVNVNEEKRHPADFALWKKAPKGHIMQWPSPWGMGYPGWHIEC
                     SAMGRKYLGDFFDIHTGGVDHIPIHHENEIAQSEALLGKPAVKYWMHGEFLLVDNGKM
                     SKSLGNTYTIADLKKKGFSSLAYRYFCLNAHYRNKLNFTWEAMKAAQVSYDRFLEGAL
                     AHKKAINSVPSEVIAAFKKEFEEAITDDLNIPKALGIAWNVVRYDSKSKELYDLLMEF
                     DNVLGLGIDKAEEKALDEGKGSYDELSPEIQELIDKRQKARKEKSWKVADEIRDKLKD
                     MGILLEDTPQGVKAVKRDS"
     gene            6161..6580
                     /locus_tag="PK733_08165"
     CDS             6161..6580
                     /locus_tag="PK733_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOJ10553.1"
                     /translation="MEEFFRNTLDEFDFHGKDIRDYSPLVLAYLGDAVYEVFIRTLVV
                     SKNDAPVCRLHKCSTAYVKAKAQSDTIHNIIEHLTQEEQDIVRRGRNAKSATIPKNAD
                     ISEYKYATGLESLLGYLYLKKDYQRLMKVLKMCVEDS"
     gene            6919..7710
                     /gene="rlmB"
                     /locus_tag="PK733_08170"
     CDS             6919..7710
                     /gene="rlmB"
                     /locus_tag="PK733_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOJ10554.1"
                     /translation="MNSALDNVVVNAERTDDRELDIIEGRNPVIEAVKSGRSINKILI
                     SKGEKEGSINYLVAIASEKKIVVQEVERAKLDKLSITGAHQGVIAFVSPIDYVELEDI
                     LEIAQSSGQQPFILILDGITDAQNLGAILRTADAAGVHGVVIPKRRAIGLTAAVAKSS
                     AGAVEYVPVARVTNIPQTIEYLKKKNIWVAGTDSAPENKSFQKSDLKGPIALVIGSEG
                     EGLGRLVKEKCDFIVNIPMKGKISSLNASVAAAIVMYEVLRQRDD"
     gene            7725..8234
                     /locus_tag="PK733_08175"
     CDS             7725..8234
                     /locus_tag="PK733_08175"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ10555.1"
                     /translation="MEYLLVDGYNIINAWKEDFKLDQESLEECREKLLNILSNYEGYG
                     KLKIIVVFDAHMVKGSQKKVEQHDNITVVYTEENQTADNYIERFVYLYGNIHNIRVAT
                     SDYLEQTIVLKHGAARMTPRELKQQIESIHRNMTNEFGSKPKKERNMLMSNISQDLLE
                     ELEKIRRSK"
     gene            8284..8943
                     /locus_tag="PK733_08180"
     CDS             8284..8943
                     /locus_tag="PK733_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10556.1"
                     /translation="MKKWLDILLFPRSLYKKISDKKLTLYIGIIFIGLVDLGFPLADN
                     FKSFYTGDGYSRIVYNIALTIIFTILLGAIDILFFSIPISDLFKRFKKGNKPADGNQV
                     YEKGLFIKLIKVYMIAHFLILLPEIISFIIYRNIANTLVSGVTTNNLNSVLLYIAFFI
                     DVLIPFWFSGAITRGVDVVYEFKPLYKRLVFLVIFVWNYLLGYALSYMISNWILKLFR
                     V"
     gene            9078..9152
                     /locus_tag="PK733_08185"
     tRNA            9078..9152
                     /locus_tag="PK733_08185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9111..9113,aa:Arg,seq:ccg)
     gene            9258..10235
                     /gene="dusB"
                     /locus_tag="PK733_08190"
     CDS             9258..10235
                     /gene="dusB"
                     /locus_tag="PK733_08190"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOJ10557.1"
                     /translation="MKLRTVKIGNVNIDNNVFLAPMAGITDRPFRLLCKEQGCGLVYT
                     EMVSAKGIHYGDIKSHKLAEINQDERPACVQIFGSEPDIMAEIARNLDSSDADLIDIN
                     MGCPTPKITKGGEGSALMLDLELAGKIVKAVSSATNKPVTVKMRKGWDESHITAVEMA
                     KIAEENGAKAVAVHGRTREQFYSGKADWNIIAEVKKAVSIPVIGNGDVFNPHDAERMF
                     QHTGCDAIMIGRGTQGNPWIFRRVIHYLKIGELLQEPSKSDKYEMIKRHLDMIVEEKG
                     EYTGVKEMRKHIAWYIKGMYNSSCVKDQIFKLNSRSEIIKILEGYFMSY"
     gene            10530..12806
                     /locus_tag="PK733_08195"
     CDS             10530..12806
                     /locus_tag="PK733_08195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ10558.1"
                     /translation="MNLEQLLDYIKNNERIVRNITYWERIPSKEAQFEDFPANIDIRI
                     KKALEKKGIYKLYSHQAAAISKISQGKNIVVVTPTASGKTMCYNIPVLDAIVKDEESR
                     ALFLFPTKALAQDQLAELHELITQAGVEVKTYTYDGDTPQSARKAIRQAGHIVVTNPD
                     MLHSGILPHHTKWTKLFENLKYVVIDEIHHYRGVFGSHMANVLRRLERICEFYGSNPQ
                     FICCSATIANPGELAGRITGSEIEVIDNNGAPSGEKHIIFYNPPVVNKELGIRRSSAL
                     ETKYLAEMLIKNGIQTIVFTRSRLNVEVLVTYLKDIFRGKMGQGDSVRGYRGGYLPNL
                     RREIERGMRKGEITGIVATNALELGIDIGSLEACIMCGYPGTIASTWQQSGRAGRNNS
                     ISAAFLVASSSPIDQYIISNPDYFFGRSPENGLVNPDNLAILLNHIKCAAFELPFSDD
                     EKFGVETTQEILSYLEQAKILRHVGKRWHWMSEVFPADDISLRSASNENFIIIDISSP
                     DRRVIGECSRFSAPMLIHEEAIYIHEGQQYQVEKLDFEEKKAYVRAVDVDYYTDANLA
                     VDLKVIDVFREEENNNILKSCGEVMVSSLVTMFKKIKFYTHENIGSGPVNLPETSMHT
                     TSYWISFPEELPEDMTQTDIQNGLLGLSNVLSNSAPIYLMCDPRDISVVYQVKSTFTQ
                     RPTIYVYENYPGGVGFSDKLFELHEEILLTAKRMIVQCGCESGCPSCVGPLNEFAGSE
                     NPKKTTMKLLELAESKKQ"
     gene            12845..14197
                     /locus_tag="PK733_08200"
     CDS             12845..14197
                     /locus_tag="PK733_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOJ10559.1"
                     /translation="MERDIISKLKLYMEQSKTVTETVTGDHSQYTGHKAHTDNLAGQG
                     IKDVNTSSGSKDNCSKYHKYDIDKILAGYIWEDTENEENEVSSCFILESQFPLSYIHG
                     GYPVGKALDIDADSLKRLCPDIMCSNSISDFLFLDTETTGLSGGTGTVAFLIGTGFFK
                     DDVFIVRQYFMRDYDEEIAMLKALNHLAVSYKGLVTFNGKAFDWNLMQTRYTFSRIKI
                     PIEEILHIDLLYPARRIWGSSLDSCSLSSLEENILGEYRVNDIPGAMIPSVYFKYLED
                     RDAREIKRVIKHNEQDVLSMVSLLLKINDLLQNPIANTECEHELLGLGRIFEACGENE
                     NFIECLENCLNSENIFVKETASKKLSQIYKKTGNYEKAVEYWNSMISDRETLNIYPLI
                     ELAKYYEHKEKNIFKALEITEKAVQLSLKMGINNSIYREDLKKRLTRLTRKAGKIDNA
                     "
     gene            14190..14696
                     /locus_tag="PK733_08205"
     CDS             14190..14696
                     /locus_tag="PK733_08205"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOJ10560.1"
                     /translation="MHDDIVQVAGISKGKFSSESQVEMTEMVLPNDTNILGNLLGGRL
                     MHWIDIVGAMAASRHSNRVVATVALDSLDFRHPVRCGELLILKAKLTWVGRTSMEVTV
                     RVYAENYRTGAVIQTNKAYITYVALDDNGIPTPVPPLILQTEEESLEYNEAEKRRNAR
                     LEHKKSEI"
     gene            14841..16451
                     /gene="spoVB"
                     /locus_tag="PK733_08210"
     CDS             14841..16451
                     /gene="spoVB"
                     /locus_tag="PK733_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HOJ10561.1"
                     /translation="MAKRKKSFVSSAIILMIAGLVVRLFGFVYRVYLSNLIGSEGIGL
                     FQLISPVYSLIILTLTSGISIAVSRLVAHEAARGHNVNIRRITLSGLMVVVAAGIVVS
                     LLILFNINFIVNSVLKDSRTYSSMIFLIPCIPVIATASVLKGYFYGMQEVTPTALSQI
                     AEQVMRISLVFFISKRFQGMGLEFACAMATAAIAIGEITNLLVLFIMYKPSIKKVTKN
                     RSKKGLMRKRRIMWEISKIAVPVSSNRLITSLMSAVELILIPRRLLTGGMDYQTSIEL
                     YGKLTGMAMPLVFFPSIFTSSLSTTLVPAISEALSLKNLKTLNYRISKSIQMTFILGF
                     LFTAIFMVYPNEISNSIFTKANVGELLYLLSFTCLFFYLQQTLLGILNGLGKEATILS
                     NSIISYAIRIVFVYFLLPLYGIKSYIIAIIISSVFVCLCNLAVVMKTTGMSIDLRNWI
                     IKPGMVCMIMIFIGKYIYSFFTLFMEHELFIAAFSVAANFAIGLILMTVTGVVSYREV
                     LKMLGLEKLPFAINIGQKLKLTNPKIRN"
     gene            16744..17214
                     /locus_tag="PK733_08215"
     CDS             16744..17214
                     /locus_tag="PK733_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ10562.1"
                     /translation="MFGLVPYRRNFGLKRKDGFNDIADMVDSFFNDSPLAPFISEINP
                     LRGNVNSMRADIKETDDEFIVDVEVPGVKKEDIKLDLRDDVLSICVEQNEEVSEEKDN
                     YIRKERRYGSCSRSFYVPDIKHDAVSAKYADGILTVTLPKADEVKQKNRKIDIE"
     gene            complement(17477..>17877)
                     /locus_tag="PK733_08220"
     CDS             complement(17477..>17877)
                     /locus_tag="PK733_08220"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOJ10563.1"
                     /translation="NLPTHAESIAYIKFLEESGIEYVASYIRWIYIRRKSSDGVFDIY
                     SDADSKIKHYKRVNTLWSALMWSEFVIGISNIIAGVEDVDKTNIWNSLNYINLFSGIF
                     LVMIGLLFFRLGSPIRKKIRRLQQEKVIIE"
BASE COUNT         6103 a         2803 c         3685 g         5286 t
ORIGIN      
        1 tgaagcactt tccctgtgcc tattattaac gtaaatatac aaagttacat attttttcaa
       61 ctgttttcat caaagctatt aattctatca ctgtcattat tttcattagg cctattaatc
      121 tcattgattc ctttaacctc atcaatctca tcaatctcat caatctcatc aacctcatca
      181 accacatcaa cctcattaat ctcgttaatc tcgttaatct cgttaacctt gttagccttg
      241 ttcgcctcat tagtgctatt ggtttcatta gcttcattcc ttacaacact acgaatgtta
      301 tacttgttat gtgaaacaaa agtatgctga gccatagact ttatttgctt taattcattc
      361 tccaggaaac tgattctgat tgaatacttg tctagttctt ttagaatatt tttatgaaac
      421 aggttaattc tttgaatttc tgcatttaaa ctattattgg attctgaaag atcttcaata
      481 ttttcactta aactctttat gttatcagat attaaattcc ttaatccctc cttatcggta
      541 ttggtattat cagtattaag taaatcctgg tagaaagcca tttttaaacc gtttatgaaa
      601 cttccgggta tatcgccagc tgaaatcctg ttgacttcgt aaaagtcgtt ggttccatat
      661 gaaatgcatg caagttgccg ccccacgggg aaattatatc tgcccgattt actccaggaa
      721 ttacccttat cgcttgaata gcaataatat attacatcat ttttaaccca ataaactaca
      781 actttatcgt caatacaaat aattgtagag ttatcaaaag gatttgaaga tgagtgtatt
      841 atggcttcgg atgaccattc catgctattt ggagcttttt gccggtatac cagatcataa
      901 cgcccggaaa caagattttg ataagaaaaa tgaattagac cgctgttatc tatagcggta
      961 gatgggtaat cagtatttat gccaggcgta gatattgaaa taaattcctc ccggagatta
     1021 tgatgagtag aaaatctact ataacccaat tgtaaggagg aattcttgtg taaagcccct
     1081 gttactgctg gttgataaaa agtataaata ttacctgatt tatctgcaac agcagtatat
     1141 ggcaaattca tcatgcttat gtaaccaact gcctggggat tttgaacttc tttatcagat
     1201 agtatttgat ggcttagtag aaggttattc ttatgttgta gaacataaaa aaaatgtatg
     1261 gtgttcttta taggtattat ctcaaaatac ttattgtaaa gtgaaggata tctgctattt
     1321 aaaacaggca atgtaactac tgaagattca tccaatcgta tataataaat atcaccatga
     1381 atactctggt aaattatatg gaacttgtct ttagaatcca tgcaggcgct aaaggattca
     1441 tgcgcctttt tctccaagga agcaggagcc gtccaggtat ttcttttagt aagagtactg
     1501 taacagatac ctaattgttt atcataatat atattccata ttttaccgga tgattgttta
     1561 aaaacatatt gtttattagt aaaattatac atatttactt caattccttg tttatatatc
     1621 tctataaaat atatatgaaa aagaatgttt acctattaca tttatgaatt ctgtttacaa
     1681 aaaatattca ccaaaaaccg taattcacat attatgaaaa tgagagagaa taatataaag
     1741 ttcatataaa gggggttaat ccatgagctt tgataatgat aatgacaggg ttgtttccgg
     1801 gttgctgtgc gacaaggata ttgccaaagt aagagaagca gtttgcatac aagtggagaa
     1861 agtttacgat tcatgcaagg agaaagattg tattgaggat gttaaggtat tgttcaagca
     1921 ctatcataaa gtacaaaaga taattaacaa ttcaattaat gtaaaatgca aaaaggcaga
     1981 agtaattgat gtatatgcgg atgtagaacc ggtacctttt aaaagaggtt tctttacagt
     2041 tgatgtaaaa ttctttataa aggttactct tgacttcttt gtatcaagac taagaggtgg
     2101 aactaaaatt tgtacaattc caggtatagt cttgtttgac aagaaagtta tactcttcgg
     2161 ttctgaagga aatataaaaa tattcaaatc tcactttaca gatccttgct atgatgtacc
     2221 aatcaaatcg gctttacagc aggataacca gcccatagcc aaggttgaag tggcagaacc
     2281 aattgccttg aacgcaaaga tccaagacct tatggataaa tgctttgaag atacttgctg
     2341 ctttgatcaa ttcccgagag gggttgcaga ggccctggat gcagataatt ttgatgacag
     2401 tgacatagtt gaaatagcag gtgaagatga gaggtgctat cccacacgca gagtggttgt
     2461 aacaataggt ctgttctcca tcattaagct tgtgagagat gtacagctgc ttattcctgc
     2521 ttttgacttc tgtgttccga agaaaaaatg tatagcttca actgaagaaa atccttgtga
     2581 actgtttgaa acaatagaat tccctgttga tgaattcttc ccgcctcaga agtttgattt
     2641 tcccagagct cttgaggaag aggctgtaat gcagagaaaa gcagaagaat aggactgata
     2701 tggtctatat acaggggaat ccggagactt acaccgcatg ctgcttaaaa ggaaaatcag
     2761 attataaaag gaaaatcaga ttataaaagg aaaaacaggg tagccttagt tgggtagtat
     2821 aaatacaggc agccctgttt tcttacttgc acttacattt agtatgattt catgttatgc
     2881 caaatttgat acaacatttt gatttattga cattagtatt ccaagttaaa tgcttacatt
     2941 gctctgcccg ggattatcaa atctactaac ccataaacaa gtgctccaat tatagctccc
     3001 caaacagtaa cattaaatcc tgcaacaaaa aattgagtaa catatattat tactgctgca
     3061 agaataaacc ctgttaatcc tctaccaaat ggtgttgcat taacacctag aactctcagt
     3121 acaaggtagt ccagtatggc gaggacgata gctccaaata taaggggcca tatacctgat
     3181 atggtaaatc ccggggttag agctgctgta attgccaaaa ctacagcacc aacaataagc
     3241 ctgatgataa aatgtgttat attgaaagtg cccgtttgtg cttgtgtatt ctcatattga
     3301 ttatccattt attcacgtcc tttctatttt ggcttattac tattatttta ttttatttta
     3361 ttatttattc agcgaaataa ttttgattag tgtgtaaata tagaaccttt acataataaa
     3421 taaatattgg tagaattaat ttgaatttta aaagggaggt taacatgaaa cttaatttat
     3481 ggctgaataa tgagatgccg gatatagcaa aatctcttga aggaataaat aacatttatt
     3541 attttaatca aaagactatc gggctttcag ggaaggaaaa aatttacaaa ataattcatg
     3601 atgtacggtc cgcccttttt cccggtgttt atgaaaaatg tcctatagat gaaaccagga
     3661 ttaatatttt tataggaaac aatatacgtg aagctgcaat agagctaagc ggccttattg
     3721 aaaaaacatt aattgatatt tgtgatactg aagataagga gactcgcaat tgcacaaaat
     3781 gcaggagcag ggccaatgac ataacaatcc accttatgaa gaagcttcct gaaataaggg
     3841 aaaaacttca tatggacatt caggctgctt atacgggaga tcctgccgct cttacaacag
     3901 aagaaattct tttaagttat ccctctatag atgctataag catctataga atagctaata
     3961 ttttatatga acaaaaggtt cccataattc ccagagttat gtctgagtat gctcaccaga
     4021 ggactggaat agacattcat ccaggtgcaa aaattggaaa atatttcttt attgaccatg
     4081 ggacaggagt tgttatagga gaaacctgca ccatagggaa taatgttaag atatatcagg
     4141 gtgtaacact gggagctaaa agttttcagc tggatgagca tgggaatcct gtaaagggag
     4201 ttaaaagaca tcctgatatc gaggataatg ttgtaatata cgcaggcgcc acaattctgg
     4261 gaggagatac ggtcataggt catgattcaa taattggcgg caatgtttgg cttacacatt
     4321 ctgtacctcc gtattccaaa gtatataatg cgcagccatc tcccataata aaacagggga
     4381 agccggagtg aaaatgcgta tgcgggcaac tacctctttt attcgcttgg ataatgctgt
     4441 ataatatcct catataatcc ataagtgagg atattatgtg cctccggagg aatttatgcg
     4501 cttgcgaata tttttgcttc gcaaaaaacg cacatgtaca attataccat gaattattta
     4561 atccatggta taataagaaa catagtgcag taaacgaaag gagttaaaca atgaaggtat
     4621 ataacacctt aaccagaaaa aaagaagaat ttgagtctat acaaagtgga aaagtacgta
     4681 tttattcctg cggaccgaca gtttacaact atgctcatgt aggaaacctc aggacatatg
     4741 tgtttatgga tatattaagg agagttttga aatatgaggg ctatgagttt aagcatgtta
     4801 tgaatataac ggatgtgggg catcttgtat ccgatgctga tgagggagaa gataagctgg
     4861 taaagaccgc aaaagagcaa aaaaagtctc cgtgggaaat agctgattat tacactagaa
     4921 tattttttga agatattgat tctttaaaca tagaaagacc ggagatcatt gccaaagcaa
     4981 cggagcatat aaaagaaatg cttgagttcg tggaaggtct tgttgaaatg ggctacggtt
     5041 acgaaaccag tgacggaata tattttgata taggtaaatt tgaaggctat ggaaaattgt
     5101 caggaataaa ccttgaagaa cagcttgcag gagcaagagt caatgttaat gaagaaaaaa
     5161 ggcaccctgc tgattttgcc ttatggaaaa aggctccaaa gggacatata atgcagtggc
     5221 caagtccatg gggtatgggg tatccaggat ggcatattga gtgttccgcc atgggaagaa
     5281 aataccttgg ggactttttt gacattcata ctggtggtgt tgatcatatt ccaatacacc
     5341 acgaaaatga aatagcacaa tctgaagctt tattgggaaa accagcagta aagtactgga
     5401 tgcatggaga gtttctgctg gtggataacg gtaaaatgtc gaaaagtctg ggtaatacct
     5461 atacaatagc agatttaaag aaaaagggct ttagctctct tgcctatagg tacttttgct
     5521 tgaatgccca ttatagaaac aagctgaatt ttacatggga agctatgaag gctgcacagg
     5581 tttcatatga tagatttctt gaaggagctc tggctcataa aaaagccatc aacagtgttc
     5641 catctgaggt tattgcggct tttaaaaagg aatttgaaga agctattacc gatgatctta
     5701 atatacccaa agctcttggc atcgcgtgga atgttgtaag atatgatagc aagtcgaaag
     5761 aactatatga tttattaatg gaatttgaca atgtgcttgg actgggaata gacaaggctg
     5821 aagaaaaggc tttggatgaa ggaaagggca gttatgatga actaagtcct gaaatacagg
     5881 aattaattga taaaagacag aaggctagaa aagagaaaag ttggaaggtt gccgatgaaa
     5941 ttagagataa gctgaaggac atgggaatat tgctggaaga tactccccag ggggtaaagg
     6001 cagtgaaaag agacagttaa agcaatgcac ccaatgcaaa acagcattat aacgaggcgg
     6061 tagatatccc ctatctctat aggtggtggg taccgtatct gttttcaggc ggtgagtata
     6121 tatcctgcgt ttataaaagt tatacgaaag gagttgcggg ttggaggagt tttttagaaa
     6181 tacattagat gaatttgatt tccacggaaa agatataagg gattattcac cccttgttct
     6241 tgcttatctg ggggatgctg tatatgaagt ttttatacgt actttggtag tttcaaaaaa
     6301 tgatgcaccg gtatgcagac ttcacaaatg ctcaacagct tatgttaaag caaaagcaca
     6361 atcagatact attcataata ttatagagca tttaacacag gaagaacagg atattgtgcg
     6421 ccgggggcga aatgccaagt cggctacaat tcccaaaaac gctgatatat ctgagtataa
     6481 atatgctaca ggtttggaat cattgcttgg ctacctatat cttaaaaagg actaccaaag
     6541 attgatgaaa gttctgaaaa tgtgtgttga agacagctga taatagaatg cacccatggg
     6601 aaacataata atgtggttta cacagataac gattaagaag gaacaggtaa ttgtatggga
     6661 aaaagggatt atagaaacaa aaaggaaaac gaagcagaaa gtcgggatga ggggaaaaga
     6721 aggagacagg gagaaaaatc tcggcaggaa cataggtata gcagcaatat tggcaaagga
     6781 aataaagctg gcagggccaa taacaatagt tataaggctt tgggcaaata tgaagacaaa
     6841 tacaaagaca aatataaaga caaatataag ggcaaatatg aggacaaata caagtgcaaa
     6901 tacgataaag aaactgtaat taatagtgct ctagataacg tagttgttaa tgctgaaaga
     6961 accgatgata gagaattgga tataattgaa ggcagaaacc cggttataga ggcagtaaaa
     7021 tcaggcagaa gtattaataa aatactgata tcaaagggtg aaaaagaggg ttcaattaat
     7081 tatcttgtgg caattgcatc tgagaaaaag attgtggttc aggaagtgga gagagcaaag
     7141 ctggacaagc tttccataac cggagctcat cagggtgtaa ttgcattcgt ttctcccata
     7201 gattatgtag aacttgagga tattcttgaa attgcccaat ccagtggaca acaacctttt
     7261 attctaattt tggatggaat aaccgatgca caaaacctgg gagccatatt aagaactgct
     7321 gatgctgcag gagtgcacgg agtggtcata cccaaacgaa gagcaatagg gcttaccgct
     7381 gctgtagcca agtcgtcggc gggagccgtg gaatatgttc ctgtagcccg cgtaaccaat
     7441 attccccaga ccatagagta tttaaagaaa aaaaacatct gggttgcagg tacagactca
     7501 gcacctgaaa acaaatcttt tcagaaaagt gatttaaaag gtcctattgc tctcgttatt
     7561 ggcagtgaag gagaagggtt gggaagactt gttaaagaaa aatgtgattt catagtaaat
     7621 attcccatga aaggcaaaat cagctcgtta aatgcttcgg tagctgcagc aattgtaatg
     7681 tatgaagtat taaggcagag agatgattga ggtttaactg aaaaatggag tatctgctgg
     7741 tagatggata taatataatt aatgcctgga aggaagattt taaactggac caggaatccc
     7801 ttgaagaatg cagagaaaag ctcctcaaca tattatcaaa ttatgaagga tacggaaaat
     7861 taaaaatcat tgtagtattt gatgctcata tggtaaaggg gagccagaaa aaagtagagc
     7921 aacatgataa cattacagtt gtttataccg aagaaaacca gactgccgat aattatatag
     7981 aaaggtttgt ttatttatat ggaaacatcc ataatattcg agtcgctaca tctgattatt
     8041 tggagcaaac aatagtgttg aaacatggtg cagcgagaat gacgccacgg gagctcaaac
     8101 agcaaataga aagtattcat aggaatatga caaatgagtt tggcagcaag ccaaagaagg
     8161 aaagaaacat gttaatgtcg aatatatcac aggatttgct tgaagaactt gaaaagataa
     8221 gaagaagcaa gtgatatgga tgggatgtaa gtaatattat taaaaatgca gggagtatat
     8281 gttatgaaaa aatggcttga tatattatta tttcctagaa gtttatacaa gaagatatct
     8341 gataaaaagc ttacccttta tatagggatt atttttattg gattggtgga tttaggcttt
     8401 cctttagcag ataattttaa aagcttttat accggtgatg gttatagcag aatagtttat
     8461 aatattgccc ttactataat atttactata ttattgggtg ccattgacat tttgtttttc
     8521 tcaataccta tatccgactt gttcaaacgc tttaaaaagg gtaataagcc tgctgacgga
     8581 aatcaagttt atgaaaaggg actttttatt aaacttataa aggtttatat gatagcacat
     8641 tttttaatac tactgccgga aataatttct tttataattt accgcaatat tgccaatacc
     8701 ttagtttccg gagtgactac taataacttg aattctgttc ttttgtatat agcatttttt
     8761 attgatgttc tcataccttt ttggtttagc ggggcaataa caagaggggt ggatgtggtt
     8821 tacgagttca agcctttgta taaaagactg gttttccttg ttatatttgt gtggaattat
     8881 ttgctgggtt atgctttgtc atatatgata tcaaactgga tacttaagtt gttcagagtg
     8941 taaaaaaagt gccctagaaa aaatagcatg gatttattgc tattttagca aatttagttt
     9001 atactagagt agtgaattta atagaagttt ttattagccg attatttttt ataaattgtt
     9061 ttatatgtat tttataggtc ccggtggcct aatggataag gctgtagatt ccggatctac
     9121 caatgcgggt tcgattcctg cccgggacgc catagtgaat aaattgcctc caatatatcg
     9181 aaagtattgc agaggctttt tcttttaaga acatataacg tgtattataa tatttgcaaa
     9241 agtggattgg agatgctatg aaactccgga ctgtaaagat aggaaatgta aatattgata
     9301 acaacgtttt tcttgcacca atggcaggga ttaccgatag gcctttcaga ttgctttgca
     9361 aagaacaggg gtgtggactt gtttatacgg aaatggtaag tgctaaaggc atacattatg
     9421 gggacataaa aagtcataag ctggctgaaa taaaccagga cgagagaccg gcatgtgtac
     9481 agatttttgg ttctgaacct gatattatgg ctgaaattgc ccgaaacctt gattcatccg
     9541 atgcagatct tattgatatt aacatgggtt gtcctactcc caaaataaca aaaggtggtg
     9601 agggcagcgc tcttatgctg gaccttgaac ttgctgggaa gatagtaaaa gcagtgtcgt
     9661 cggctacaaa taagccggta actgtaaaga tgaggaaggg atgggatgaa tctcatatta
     9721 cagctgttga aatggcaaaa attgctgaag aaaacggtgc aaaagctgtt gctgtccatg
     9781 ggagaacaag agagcagttt tacagtggga aagctgactg gaatataatt gctgaagtta
     9841 agaaggcagt gtctatacca gttattggaa atggagatgt tttcaaccct catgatgcgg
     9901 agcggatgtt tcagcatact gggtgtgacg caattatgat aggtagaggt acacagggca
     9961 atccatggat atttaggaga gtaatccatt acctgaaaat aggggagctg ctgcaggaac
    10021 catccaaatc agataaatac gaaatgatta agcgccattt ggatatgatt gttgaggaaa
    10081 aaggtgaata tacaggtgtg aaggaaatga gaaagcatat tgcctggtat ataaagggca
    10141 tgtataattc cagctgtgtg aaggatcaga ttttcaaact caattcaagg agtgagatta
    10201 taaagatttt ggaagggtat tttatgtctt attagaatcc tttacttttt cattaatata
    10261 taatacaatt taattgttag aagaatatat gttccacaaa ttttataaat attagaggtt
    10321 gtagtgtgaa agactttaat ctgaaacgct atgcacatct gcctgtttta gaggataggg
    10381 gtgaaaaaat agatgatgca caagccaaat aatctgggtg tgcattatgt tacattaatg
    10441 tattcttttg caagattatt aattcagtaa attttgcaaa acaattaatg ggacaattca
    10501 tttggtacat tggggacatg gaggttttta tgaatcttga acagctgctt gactatatta
    10561 agaataatga aagaatagta cgcaacataa cttattggga aaggatacct tcaaaggaag
    10621 ctcaatttga agattttcct gcaaatatcg atattagaat aaagaaggct cttgaaaaaa
    10681 aaggtatata caagctgtat tcacatcagg cagcagccat atcaaaaatc agtcaaggca
    10741 aaaacattgt agttgtaaca cctacagctt ccgggaaaac tatgtgctac aatatacccg
    10801 tactggatgc aatagttaaa gatgaggagt ccagggctct atttctcttt cctacaaagg
    10861 cactggccca ggatcagctt gcagaacttc atgaacttat aactcaggca ggtgtagagg
    10921 taaaaactta tacatatgat ggtgatactc ctcaatctgc gagaaaggca ataaggcagg
    10981 caggtcatat tgttgttaca aatcctgata tgcttcacag cggtattctc ccgcatcata
    11041 caaaatggac aaaattattt gaaaatctta agtatgttgt tattgatgaa attcaccatt
    11101 acaggggagt attcggaagt catatggcca atgtcctcag gcggttggag cggatatgtg
    11161 aattttacgg atccaatcct caatttatct gttgctcagc cacaatagcc aatcccggtg
    11221 aactggcagg aaggataaca ggctctgaaa tagaggttat tgataataat ggggctccct
    11281 caggcgagaa acacataata ttctataatc ctcctgtagt aaataaagaa ctgggaatac
    11341 gcagaagcag tgctctcgaa acgaaatacc ttgcagaaat gctcattaaa aacggaatac
    11401 aaacaatagt atttacaaga agcagattga atgttgaagt gctggttaca taccttaagg
    11461 atatatttag gggcaaaatg gggcaagggg acagtgttag aggttataga ggagggtatc
    11521 ttccaaacct gagacgggaa attgaaagag gtatgaggaa aggcgaaatt acggggattg
    11581 ttgcaactaa cgctttagaa ctggggattg atataggcag cctggaagcc tgcattatgt
    11641 gcggttatcc cggtacaatt gccagcacat ggcagcagtc gggaagagcg gggagaaaca
    11701 acagtatatc agctgctttt ctcgtagcaa gcagcagtcc cattgatcaa tatattataa
    11761 gtaatcccga ctattttttt gggagaagtc ctgaaaacgg tcttgtaaat ccagataacc
    11821 ttgccatatt gcttaatcac ataaagtgtg cggcatttga actacccttc agtgacgatg
    11881 aaaaattcgg agttgaaaca actcaagaaa tattgtcata ccttgaacag gctaaaatct
    11941 taagacatgt aggtaaaagg tggcattgga tgtcagaagt gttcccagct gatgatatca
    12001 gcctgagaag cgcatctaat gagaatttta ttattataga tatttcttca cccgacagga
    12061 gggttatcgg agagtgcagc cggttcagcg ctcccatgct tattcacgag gaagccattt
    12121 atatacatga gggacagcag taccaggttg aaaaacttga tttcgaagag aaaaaagctt
    12181 atgtaagggc ggttgatgta gattactata ctgatgcaaa tctggctgtg gatcttaaag
    12241 tcattgatgt gttcagggaa gaagaaaaca ataatatttt gaaaagctgc ggggaggtta
    12301 tggtgtcttc cctggttaca atgtttaaaa aaattaaatt ttacactcat gagaatattg
    12361 ggtcgggacc ggttaacctt ccggaaacca gcatgcatac tacatcttat tggataagtt
    12421 tccctgaaga gcttcctgaa gacatgacac agaccgatat tcaaaacgga ctgctgggat
    12481 tatccaatgt tttatccaat tccgcaccca tctaccttat gtgtgatcca cgggatatta
    12541 gtgttgttta tcaggtgaaa tcaaccttca ctcaaagacc caccatatat gtttatgaaa
    12601 attaccccgg aggtgtaggc ttcagcgata aactttttga attgcatgag gagatacttc
    12661 ttacagcaaa gaggatgata gtacaatgtg gatgtgagtc cggctgtcca tcctgtgtag
    12721 gacctttaaa tgaatttgcc ggaagcgaaa acccgaagaa aacaacaatg aaactactgg
    12781 aactggctga aagtaagaag caataagcaa gtagtgtaaa gttggaagtg agtagtgaga
    12841 gattatggag agggatatta taagtaaact caaactttat atggaacagt ccaaaaccgt
    12901 aactgaaacc gtaacaggag accattcaca atatacagga cataaagcgc atacagataa
    12961 tttagcggga cagggaatca aggatgttaa taccagttcc ggcagcaaag acaactgcag
    13021 caaataccat aaatacgata tagataaaat acttgccggc tatatatggg aggatacgga
    13081 aaacgaagaa aatgaagtca gctcctgctt tattctggaa agccaatttc ccttgtcata
    13141 tatacatgga ggttaccctg ttggaaaagc tctggatata gatgcagatt ccttaaaaag
    13201 gttatgccct gatattatgt gttccaattc aatctcagat tttcttttcc ttgacactga
    13261 aactacaggc ctctcgggag gaacagggac agtagctttt cttataggca caggtttttt
    13321 caaggatgat gtgtttatag taagacagta ctttatgaga gactatgatg aagaaatcgc
    13381 catgttaaag gccttaaatc atctggcagt ttcatataag ggccttgtta cctttaacgg
    13441 caaagcattc gactggaacc tgatgcagac caggtatact ttcagcagaa taaaaatacc
    13501 catagaagag atactgcata ttgacctttt atatccggca aggcggatct gggggtcaag
    13561 tcttgatagc tgttctcttt cttctcttga agaaaatata cttggagaat atagggttaa
    13621 tgatataccg ggagcgatga taccttcagt ttattttaaa tacctggaag acagagatgc
    13681 ccgtgaaata aagagagtaa taaagcataa tgaacaggat gtcctttcca tggtgtcttt
    13741 gcttttaaaa ataaatgatc tcttgcagaa tccaatagcc aacactgaat gtgagcatga
    13801 attgcttggc cttggcagaa tttttgaagc ctgcggggaa aatgaaaact tcatagagtg
    13861 tctggaaaat tgtttgaact cagaaaatat ttttgtaaaa gaaactgctt caaaaaaact
    13921 ttcacaaata tataagaaaa ccggcaacta tgaaaaggca gttgaatact ggaacagcat
    13981 gatatctgat agagaaacgc ttaatatata ccctttaatc gaattggcca agtattatga
    14041 gcataaagaa aaaaacatat ttaaagcact tgaaattaca gaaaaagctg ttcaactctc
    14101 tttaaagatg ggaataaata atagtatata tagagaagat ttaaaaaaac gtttgacccg
    14161 attaacgaga aaggcaggta aaatagacaa tgcatgatga tatagtacaa gttgcaggta
    14221 ttagtaaagg taaattttca tcagaatcac aggtcgaaat gactgaaatg gtgcttccaa
    14281 atgatacaaa tattcttgga aatttacttg ggggaagatt aatgcactgg atagatatag
    14341 ttggggctat ggcagcttca agacactcca acagagttgt agcaactgtt gcacttgata
    14401 gtcttgattt caggcacccg gtaagatgtg gagaattatt gatcttaaaa gccaaattaa
    14461 catgggtggg aagaacatcc atggaggtaa ctgtgagagt ttatgcagaa aactatagga
    14521 ccggagctgt aatacaaaca aataaagctt acataaccta tgttgcactt gatgataatg
    14581 gcattcccac tcctgtacct ccacttattc tccaaactga agaagaaagc cttgagtata
    14641 atgaagcgga aaagagaaga aatgcacgtc tcgaacacaa aaagtcagag atatgatgca
    14701 ggacaaatct tcgactcatg acctgaaaat taatataatg aactgtttat tacatcaaat
    14761 tttttctttt taatcatatg ttgtgggaat tattaataat tatatcatga cacttatatt
    14821 aaagaggggt catgataatt atggcaaaga gaaaaaaatc atttgtaagt agtgcaatta
    14881 ttttaatgat agccgggctt gttgtccgtt tatttggttt tgtttacagg gtatatcttt
    14941 caaacttgat aggttcagag ggaataggcc ttttccaact tatatcaccg gtctattccc
    15001 tgataatttt aacgcttact tcgggaatct ctatagctgt ttccaggttg gtagcacatg
    15061 aagcagcaag agggcataat gtaaatataa gacgtattac tttaagtggg cttatggtag
    15121 tagttgctgc gggtattgtg gtatcgcttc ttattttatt taacatcaat ttcatagtaa
    15181 attcggtatt gaaagactca agaacatata gttcgatgat ttttctcatt ccatgtatac
    15241 ctgttatagc tacagcatct gtcttaaaag gctattttta tggaatgcag gaggtgaccc
    15301 cgactgcctt gtcacagata gcagagcagg taatgagaat atcattagta tttttcattt
    15361 caaaaaggtt ccagggtatg ggattggaat ttgcttgtgc aatggcaact gcggctatag
    15421 ccataggcga aattaccaat ttacttgttc ttttcataat gtataaacca agcataaaaa
    15481 aagtcacaaa aaacagaagc aaaaaaggtt taatgagaaa acggcgaatt atgtgggaaa
    15541 tttcaaaaat tgccgtccct gtgtcctcaa acagattaat tacttcacta atgtcggctg
    15601 ttgagctaat attgataccc aggaggctcc ttacaggtgg gatggattat cagaccagta
    15661 ttgaattata tggaaaactt acaggaatgg ctatgccgct ggtatttttc ccttcaattt
    15721 tcacttcatc cctttccaca acacttgtac cggcaatatc tgaagcttta tctttgaaaa
    15781 atcttaaaac cttgaactac agaatatcaa aatccataca aatgacattt atactcggat
    15841 ttctttttac agcaattttc atggtttatc ccaatgaaat cagcaacagc atatttacca
    15901 aggcaaatgt gggggaactt ctttaccttc tttcatttac ctgcctgttt ttttaccttc
    15961 agcagacatt gcttggaatt ctgaacggcc tgggtaaaga agcgaccata ctgagcaact
    16021 caattataag ttatgcaata aggatagttt ttgtatattt tctgctgcct ttatatggta
    16081 taaaaagcta tattattgcg attattataa gctcggtgtt tgtatgtctt tgtaatctgg
    16141 ctgtagtaat gaagacaaca ggtatgtcca ttgatttgcg taattggatt ataaagcctg
    16201 gaatggtatg tatgataatg atcttcatag gtaaatacat ctacagcttt tttacattat
    16261 ttatggaaca cgagcttttt attgcagcct tttcagttgc cgctaatttt gcaataggtc
    16321 ttattcttat gacggtgaca ggcgttgtga gttatcgtga agtcttaaaa atgcttgggc
    16381 tggaaaaatt accatttgcc ataaacatag gccaaaagct aaaattaaca aacccaaaaa
    16441 tcagaaatta gaaacgaacg atatattgag gaataacacg aaaagctatg aaaaataaac
    16501 ataaatacat acaattctat aatatggtag tatattagta ttgcaccgtt agttatatct
    16561 gtgatataaa ataactagaa ggtcaaagaa agtcaaagac aaaaacaaaa aacgaataac
    16621 aaagaataaa aaggcaacaa taaaaacaaa gataagcaaa acacatgaac aaatttataa
    16681 aatacttaga aagcattaaa aataaaaaaa taattatttt aaataattca ggaggtgttc
    16741 ttaatgtttg gtttagtacc gtatagaaga aattttggtt taaaaaggaa agacggtttt
    16801 aacgatattg ctgatatggt tgacagcttc ttcaatgact ctccgttagc tccctttata
    16861 tcagaaatta atcctttgag gggtaatgtt aatagcatga gggctgatat taaagagaca
    16921 gatgacgaat ttatcgtaga tgtagaagtt ccgggagtta agaaggaaga tatcaagctt
    16981 gaccttagag atgatgtttt aagcatatgt gtagaacaaa atgaagaggt cagtgaagag
    17041 aaagataatt acatcagaaa agagagaaga tacggctcat gcagcagaag cttctatgta
    17101 cctgatatta aacacgatgc tgtatccgct aaatatgcag atggaattct gacagttact
    17161 ttgccaaaag cagatgaagt caagcagaaa aatcgcaaaa ttgatattga ataaaaagaa
    17221 agaaaagaaa aagagtgacg ggttaggcac attctcatag ggagatttta tgaaccgtgg
    17281 cgtgggtttt gggtgaggca gacagcagat caagttgtct gcctttgtca ttgtgaaaca
    17341 aagtaaacag ttccatttaa ataaatgcac cttgatatag gtaaattctt aaaaaaaata
    17401 tagatgtttt tcttaagaat ttcttacaat taattattag taatttgtaa tggtaatctt
    17461 tggttgagat gtagtgtcat tcaatgataa ctttttcttg ctgaagcctt ctaattttct
    17521 ttcttatagg tgagcctaat ctaaaaaata gtagtcctat cattaccaga aaaatcccac
    17581 taaataggtt gatataattc aaactattcc atatgttagt tttatctaca tcttctaccc
    17641 cagcaatgat attcgaaatc cctataacaa attccgacca cattaatgca ctccataaag
    17701 tattcactct tttataatgc tttatttttg aatcggcatc tgagtaaata tcaaatacgc
    17761 catccgagga ttttcttcta atatatatcc aacgtatata agaagctaca tactctatgc
    17821 cactttcttc taaaaattta atgtatgcta tactctctgc atgggtaggt agatttt
//
LOCUS       DAOSDD010000072        17780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1288_length_17780_cov_5.327165, whole genome shotgun sequence.
ACCESSION   DAOSDD010000072 DAOSDD010000000
VERSION     DAOSDD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_17780_cov_5.327165"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1483)
                     /gene="nagZ"
                     /locus_tag="PK733_08225"
     CDS             complement(131..1483)
                     /gene="nagZ"
                     /locus_tag="PK733_08225"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOJ10564.1"
                     /translation="MRSKKKMYSSTILVVLLVLAIVLVPVVLALKGYFPGRQKKDIDN
                     KGTSSNNGMSSNKGTSSNKGTSGKDMPGDITTAPEIDLTRQQINEMTLQEKIGQMVIV
                     GFDGYTIDEDIKMIIENYQIGGAILFQRNVKDAEQLLDLNNALKSINSKNKIPLFISV
                     DEEGGRVSRMPKELEKLPVSGIIGEVNNENISYEIGSILAEELKCFGFNINFAPVMDI
                     NSNPQNPVIGDRSFGSDAQIVSKLGVQMMKGIQSGGVISVVKHFPGHGDTSEDSHLNL
                     PVVSHDLNRLMNFELIPFKSAIDDNVDAIMIAHILLNKIDPENPSSLSKTVIMDILRN
                     KLKFNGLVISDDITMEAIVKNYNISDAAIRAVNAGCDIILVCHGYDNEIAVMDALKKA
                     VESNIIPLERINESVYRILKLKGKYKITDDQMDVRNLINIQNINNKIKKIWEENVEKK
                     "
     gene            complement(1748..5371)
                     /locus_tag="PK733_08230"
     CDS             complement(1748..5371)
                     /locus_tag="PK733_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOJ10565.1"
                     /translation="MRPKLLEIEGLQSFCDIQRIDFEALGETGLFGIFGPTGSGKSTV
                     LDAITFALYGKVKRADGGTQGIINANMNTVKVSFTFELVKNSTRKTYRVERVYQRKKG
                     SANSCEPKIARLIQISDVGEIPICDKATDVSNNIKELLGLSHEDFTRAVVLPQNSFQE
                     FLLLNNSERRGMLERIFYLEEYGKQLQEKLNLKMTKLKSRIDVLSGELRGYVDATDEA
                     LDEAHKMMETAVSERNRVENEWKQLESKYNEAKEVWMLVQEFGFINRKEQQHLSSKEI
                     FAEKRVQLEKAVKADGLLEMILKNRELNEKLKETEKQLEIVLNSLPDVDENLSEIRQK
                     YEYLKNEVVIEQPKLVGLRTRLADALGIRNEIKSVEGKINGFLASAAKLKGAISKNNV
                     NIRNSAGELENLEHALGKLKLEMESLKTEPEYRQQIQDGMKLENEVETLKGNVKETEN
                     RAAELNKAIAGLEQELAGISEKIKLSQRNLENLNAEILEYQKSKPEDRNLVLKYIDKL
                     RSMQVVYDVLRFRKNELDGMKSKITRQEAELGKLTEKAAVLEEAGEKAGALFEKCRKE
                     LEDANRAMDRNAAYILSKNLKEGEPCPVCGSEHHPRLAVHGEETELAALEKHIEEAGK
                     KLAGAEKAFKESEKQALVAGEQVKSVTGQIHQLMQELKTKSDEYNEEKLKLPEELKEL
                     DLDQIHLELMKMNSIGAEKLIAVEAWEKKQEEYKEELLKINNIISEHRLSENGIISEL
                     KVNRENLKQIEKSMSDAKKIFSEKQHKYSEFLQQHKIVSASFELKRLSENDRRINVLQ
                     KQMDNGQKLITDKRALLEKWREELNTLNNEHIKVQSEIDNLNAQKDEKTGKLKELAGD
                     ANIEDEIRRIDEKLDEYMRFEKQYQEKLQALEKQHNDLLSLKSLLVNQRNIYCENLVN
                     EETRLKNVMDERGFTDLSEVERAVIPSERQKTLKNEIDEYDQAGTNIKAQKEMVQKKL
                     NFRSITEEEWNHTSNDYQELASLKEECVSRSEVARNNFHNIKSKHDKWVKLNNRYKEL
                     NYKQGLFEQIQKLLRAEHRKDNSFIDFIAEERLRYVAAKASESLGFMTKYRYELELDT
                     DAGFIIRDNANGGVHRMVTSLSGGETFLTSLSLALALSEQIQLKGQSPLEFFFLDEGF
                     GTLDSNLLDSVIDSLERLSRKERVIGLISHVPELRNRISRRLIVDPPTSQGDGSRVKI
                     EKA"
     gene            complement(5352..6836)
                     /locus_tag="PK733_08235"
     CDS             complement(5352..6836)
                     /locus_tag="PK733_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOJ10566.1"
                     /translation="MRILHTSDWHLGKTLEQVNRIDEQREFIDCLCKTVEEQRIDLVL
                     VAGDIYDTYNPSAAAEELFYDAIDRLNGKGTRAVVVIAGNHDNPERLCAASPLAYKNG
                     IILLGYPGSDAGMYKISSGNIRIEDSGPGWLELCVPGCENHAVIITIPYPSESRLEEV
                     LSQQADEQILQKAYSDKVGSIFTLHSRKFREDTVNLATGHFFIRGGKESESERTLQVG
                     GALTVDPGILPSNAHFVALGHLHRPQEIRNAPCPVFYSGSPLAYSFSEAEHSKAVYII
                     DAIPGEKAKISPLYLDCGKPLRRWIAEEGIEQAIKWCEDGRDANAWIDLEIVTDRVLT
                     ADEQKALRSLHPGIINIRPRLKSEDMEILSYQSREGKKIDELFKDYFKYRMGTEISDE
                     LMGAFIEVVNGTDEEDDAGEYDDEAKYGEADEYDDEAKYGEADEYDDEAKYVEADKYD
                     DVTSYDNKDKNDYDNYNDNYNYNDNDNDNDKDGKEAEADETQAS"
     gene            complement(6895..8775)
                     /locus_tag="PK733_08240"
     CDS             complement(6895..8775)
                     /locus_tag="PK733_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10567.1"
                     /translation="MNNVIKKPFRSVLIITLSIIVLVIPLVPIHADENSVIRFSDVPE
                     GHWADNAIHELRLLKITDGIGNNKFGLGLTIKRCEFVAYLVRLMGWELVKPEKGSFID
                     NMDNSKWYYPYIETALKSGVIPSTTPNDNTTILNNDNAFRPDDPITREEMAVMIVHTL
                     GYEMLTKQLTYMESPFDDVSKNKEYIIIAKDFGIITGTGNNKFKPYNTATREEAAAMM
                     IRMYNRLNQPINELHAFYAIRSANQMDMINDLDSVGFGWSRLEYDEKNRQVVLNTTRN
                     NNNEYAVPEGFLQPYNMARQSKVSAQLMVFADNETVLDIGSNEIIVSSTEDKDKISSA
                     EDKDKISSTGDEDKVSNAIDQGEIPLLEYVLTKPEIRGQVIESIVNQVNATAKDGITI
                     SFDGVVIDFESMKGELLKQSFNSFLTELKQELDKTSKILYVAVHPKRKSGQDYYNAYD
                     YKAIGEIADKVILMAHDYYAKKLSDADMQNGYTITPLSPFDEVYYALKAITDKDSGVE
                     DLNKIWLQISFDAVQWKLKDGKVINRYPYSPSYEAIRQKLVADAEMNYSNINQNPYIT
                     FYNSGDETKNVIWYEDSRSVQAKIKLAGMFGINGVSLWRLGNVPDYDIWQQILSNKKK
                     LF"
     gene            complement(9010..9858)
                     /locus_tag="PK733_08245"
     CDS             complement(9010..9858)
                     /locus_tag="PK733_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOJ10568.1"
                     /translation="MKRFNEFLIINFGLFLVALGIYLFKIPNNFAIGGVSGIAIVVGR
                     FFPGSPVGLIMMAINILLTIAGIIFVGFSFGFKTIYSSFALSGMVWLLEKIYPISKPL
                     TRDTILELVYAVILPAVGSAIVFNKNASTGGTDIIAKILAKYMRINIGKTLLLGDFII
                     TVMAGMVFGIEIGMYSILGLFLKGFIIDFVIEELNLCKQVVIISANSDQVKKYITENI
                     KRGATVYKAIGAFTNQEKEVITTIVTRKQAVKLRDYIGNADPKAFVTITNTSEIIGKG
                     FRNVVF"
     gene            complement(10081..10800)
                     /locus_tag="PK733_08250"
     CDS             complement(10081..10800)
                     /locus_tag="PK733_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ10569.1"
                     /translation="MNMEVSNGNARYSLRYKVLGQLEESILNGEYQPGDYLVETKLSR
                     QFGVSRTPIREAIRQLEREGLVKIVTNKGAMVLGVTDEDIEDIYNIRMEIEKLAVRWA
                     IQKITPDEIEKLKEQVELAEFYINKKHCTRLQDLDTEFHRIIYDACKSRPLKHMLSDF
                     HRYVQRARKTSLQTPGRAKEALKEHKEILETIIEENVDKAEALICKHIRNAKENLLKN
                     HILLKTEEKDSIENSNNYNES"
     gene            complement(11272..11892)
                     /locus_tag="PK733_08255"
     CDS             complement(11272..11892)
                     /locus_tag="PK733_08255"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOJ10570.1"
                     /translation="MTSLIDLSHTIEDNMPVYPGDIRTNLFQIKYLSVNKYNDHRLDI
                     SMHSGTHIDSPMHLTESNEYISQASLEPFIATGCVLDVRNQAVIKMKAEYEKLIKDNS
                     IVLFYTGQDKYYGMTKYYEEHPVVGIDICRFLLEKNIKMLGMDMPSPDKYPFEIHKIL
                     FANEIFILENLTNLDKLLGVENFEVIALPLKIKSDSSMARAVARIL"
     gene            complement(11977..12756)
                     /locus_tag="PK733_08260"
     CDS             complement(11977..12756)
                     /locus_tag="PK733_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOJ10571.1"
                     /translation="MFLKNEENHIRNVVQTSLLIALAIVVRNFSYMVYLGGAPGMRIS
                     FSGVFTKITAILFGPLFGGIASGLVDITGFIIKPEGAYIPWLTVTAVSGGVITGLVWK
                     MLRRVNAKRLQVSFLLFFIIIGVLGIVNYIHVALIPASPWAQIINSIGKYRDFTTIGL
                     IVACLFGSVFILADQIFRKIYNNSQLYNDFFKILMSTGVSGIIVTTVNTYILRIFIPA
                     LGKMGFWVLWIPRVIQEILMVVIQAYVVSILLFTYKKYVRK"
     regulatory      complement(12842..12949)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(13037..14761)
                     /locus_tag="PK733_08265"
     CDS             complement(13037..14761)
                     /locus_tag="PK733_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ10572.1"
                     /translation="MKTQIEGKTQFENINIIDEPGNEDNEGNECIEVQAAEVQMTKIQ
                     IPEIQAGEVQIFEIQAGEVQISEIQAGEVQISEIQTGEIKIPEAQIAEVKTGKIQMAL
                     DLVEPGNDDAYSHGKQERVSKHLPSEVSSRMLYKDIVRIAWPSFVELTLTQLTSMVDM
                     MMVGQLGPWAISAVGLTNQPKFLMMTMFMAMNVGATALVARYKGSGDYNKANTILRQS
                     LLLTFVLSVISSIIGYIYSEPLIRFMGAADAETLAGGTIYLKIQMAGFVVMALTSTIT
                     AALRGIGDSRTAMIYNLTANGVNVVFNYLMIYGHLGFPKMEVAGASLATVIGQCVAFI
                     LAVNAILRGNQYLRLRLKDGFKPHWESMKNIFIIGIPALIEQIVMRAGMIIYSITVAS
                     LGTVAFATHQVCMNIQAMSFMNGQAFAVSATSLMGQSLGKKRPDMAQAYCSRTRRIGM
                     TVSILLGLIFFFFAKPIVALYSDDSAVIHQGAKILMLVALIQPFQSSQFILAGALRGA
                     GDTKATAVVTFITVLLVRPGLALLGIHVFGWGLEGAWYALVADQLVRSLLILIRYNSG
                     KWKTIKLS"
     gene            complement(15001..16347)
                     /locus_tag="PK733_08270"
     CDS             complement(15001..16347)
                     /locus_tag="PK733_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HOJ10573.1"
                     /translation="MNYEYDENIIKNALSTVEIPEYNIIPEVDKKIKEQKSHMNFKRI
                     VAAVLAVCLCLMFSVGLVAAAIPNFKKLLFFVASDIALMLQPVEISSEDNGIKMEVVA
                     AMNDDEMAVIYLTMQDLTGNRIDKTLDIYDYSLTGMHAFNCQIVNYDEITKTATLRMQ
                     ANGGEKLDGKKVKFRIDSFLSDKLIFDAVETGINLLDIASANVSQTVPLDMNNIPGGG
                     GSLFEEFKEQGIVKVLKADQMKHSLPGIEFMYISNIGYIDGRLHIQTKWVGEGIDDHG
                     YFYFVNTAGDKINISASNIYFGTDESGDTKYGNDYVEYIFDVNNLNLNEIKLMGFFVS
                     NGNYTTGNWEVAFKIQSVGQQKQTDCNIKFDTWTVTSISVSPMGITLTGSGENKEPQS
                     ITVSANMTDGSVQTFESVRSFSDNGKVKIKFTSSLPLDVSKVQSVTVDGIEINNKN"
     gene            complement(16344..16868)
                     /locus_tag="PK733_08275"
     CDS             complement(16344..16868)
                     /locus_tag="PK733_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ10574.1"
                     /translation="MEARELDYAFTAGGEYELHCAIELYGQPLLRYCHNILCDYFEAQ
                     DAVQITFIKAYNKRKSFKNGTSLSAWLYRIAYTTCIDSLRKKKLLFFVQQTNTDSKFI
                     SDDLKEALMTLSPAERALVFSRVIDEKSYADLEAIYNASATTLRKRYERAKTKLLKAL
                     KETNSYYARLEERK"
     gene            complement(17200..>17780)
                     /gene="ung"
                     /locus_tag="PK733_08280"
     CDS             complement(17200..>17780)
                     /gene="ung"
                     /locus_tag="PK733_08280"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ10575.1"
                     /translation="STKIIYPDKDNIFEALKLTTYKDVKVAIIGQDPYHGENQAHGLA
                     FSVQKGVDIPPSLLNIFKELVNDLNCYIPNNGCLVPWARQGVLLLNTSLTVVADAANS
                     HRNKGWEVFTDSIIQLLNKKDTPIVFLLWGNNARDKAKYITNQKHLILTSAHPSPLSA
                     NRGFFGCKHFSKANNFLRETGQTEIDWQIPNI"
BASE COUNT         5001 a         3918 c         2623 g         6238 t
ORIGIN      
        1 ttattcagat tattttgtga gtttgatttt gcccatagct gaatttaaag gctctaagta
       61 ctgtaacgac agccactgat aaatatttct tcaaaaattt taaatgcacc caaaccattg
      121 ttatttttta ttatttcttt tctacatttt cctcccatat tttctttatc ttgttgttta
      181 tattttgaat atttatcaag ttccttacat ccatttggtc atctgttatt ttatactttc
      241 cctttagctt caaaattctg tatacacttt cgtttattct ctccagcgga ataatattgc
      301 tttctactgc ttttttcaaa gcatccatca ctgctatttc attatcatag ccatgacaga
      361 ccaatataat atcgcaccct gcatttacag ctcttatagc tgcgtcagat atgttgtagt
      421 tttttacaat agcttccatt gtgatgtcat cactaattac caacccgtta aactttagtt
      481 tgtttctaag tatatccata attaccgtct ttgataatga agaagggttt tccgggtcta
      541 ttttatttaa aagtatatga gctatcatta ttgcatccac gttatcatct attgcagatt
      601 tgaaaggtat aagctcaaaa ttcataagtc tgttcaaatc atggctaaca acaggtaaat
      661 ttaagtgaga atcttcagaa gtgtccccat gtcctggaaa atgcttaact acggaaatca
      721 ctccccctga ttggatgcct ttcatcattt gtacacccaa tttacttaca atttgtgcat
      781 cagaaccaaa tgacctgtcc cctattacag gattttgcgg attgctgttt atatccatga
      841 caggagcaaa gtttatattg aatccaaaac attttaattc ttccgctaga atactgccta
      901 tttcatatga aatattttca ttgttaactt ctcctattat tccactgaca ggaagctttt
      961 ccagctcttt cggcatcctg cttaccctgc cgccttcctc atcaaccgat ataaacagtg
     1021 gtattttatt tttagaatta atacttttta gagcattgtt caaatcaagc agttgctctg
     1081 catcttttac attgcgttga aataaaatgg caccacctat ctggtaattt tcaattatca
     1141 tttttatatc ttcatctatt gtgtaaccat caaaccccac tattaccatt tgcccaattt
     1201 tttcttgcag ggtcatttca ttaatctgtt gtcttgtaag atcaatttca ggtgcagtag
     1261 taatatctcc gggcatatct tttcctgatg ttcctttatt acttgatgtt cctttattac
     1321 ttgacattcc attattactt gacgttcctt tattatcaat gtcttttttc tgccttccgg
     1381 gaaaataacc ttttaaagcc agtactactg gcaccaatac aattgccagc accagaagta
     1441 caactagtat agtactacta tacattttct ttttacttct catcttaacg cgtattcctt
     1501 tcatacttct gatttactag tatacctaca tatttacata ttattacatg gctgtaaaag
     1561 ggaacatatt cgctatgcca aaggggcatg tatattccaa gggaaatgtc cccctgctct
     1621 gactcaagtt gttgagcaca gtcccccttg caagctgttg agaaccgtcc ccttactccc
     1681 ctcgagaacc gtccccttac tcccctcccc ttacttccgc attccgcttc ccggtctctt
     1741 agcctctcta agccttctca attttaaccc tgcttccatc tccctgtgat gttggcgggt
     1801 caactattaa ccttcttgat atcctgttgc gcagttccgg aacatggctg attaggccta
     1861 tgactctttc cttcctgctc aggcgttcca gagaatcaat aacagagtcc agcaggttac
     1921 tgtctaaagt tccgaaccct tcatccagga agaaaaattc caggggactc tgtcccttta
     1981 attgtatctg ttcagatagt gccaacgcaa gagataagga tgttagaaag gtttctcctc
     2041 ccgaaaggga agtgaccatt cggtggaccc ctccgtttgc attgtccctg atgatgaaac
     2101 ctgcatctgt gtccagttct agttcatatc tgtactttgt catgaatcca agggattcgg
     2161 aggctttggc agcaacatac ctaagccgtt cctcggcaat aaaatctata aagctgttat
     2221 cttttctgtg ttcagccctc aaaagcttct ggatctgctc aaatagtccc tgcttatagt
     2281 ttagctcctt atatctgttg ttcagtttta cccatttatc atgcttgctt ttaatgttgt
     2341 ggaaattgtt tctagctacc tcgctgcggg atacacattc ctccttgaga gatgccaact
     2401 cctggtagtc attacttgtg tgattccatt cttcttcagt gatgctccga aagttcagtt
     2461 ttttctgcac catttccttc tgagccttta tatttgttcc tgcttggtca tattcatcaa
     2521 tttcgttttt taaagttttt tgcctttcag acgggatcac cgctctttcc acttcactta
     2581 agtccgtaaa tcctctttca tccataacgt ttttcaaccg ggtctcttca ttcaccaagt
     2641 tttcgcaata gatattccgc tggtttacaa gaagagattt cagtgataaa aggtcattgt
     2701 gttgtttttc cagagcctga agcttttctt gatactgctt ttcaaacctc atatactcat
     2761 ctagtttttc atcaatcctc ctgatttcat cttcaatgtt tgcatctcct gccaattctt
     2821 taagtttgcc tgttttttca tctttctgtg cattcagatt atctatttcg gattggactt
     2881 taatatgctc gttgtttaat gtattgagct cttctctcca cttttcaagc agagctctct
     2941 tatcagttat taatttctga ccgttatcca tttgcttttg caatacattt attctgcggt
     3001 cgttttctga aagccttttt aattcaaaag atgcactgac tattttatgc tgctgaagaa
     3061 attctgagta cttatgctgt ttttcactaa atattttctt tgcatcagac attgattttt
     3121 caatctgctt cagattttca cggttcactt tcaattctga aataattcca ttttccgaaa
     3181 gcctgtgttc tgaaattata ttattgattt tcaggagttc ctctttatat tcctcttgtt
     3241 ttttctccca ggcctcaact gctataagct tttcagcacc tatgctgttc attttcatca
     3301 gttccaggtg aatttgatct aaatccaatt cctttaattc ttcaggcagc ttaagctttt
     3361 cctcattata ttcgtctgat ttagtcttaa gctcctgcat taactgatgg atttgcccgg
     3421 tcacagactt tacctgctca ccagccacaa gagcctgctt ttctgattcc ttgaaagcct
     3481 tttcagcacc agccagcttt tttccggcct cttcaatatg tttttccaat gccgccagct
     3541 ccgtttcttc cccatgaaca gcaagccttg gatgatgttc tgaaccgcat accgggcatg
     3601 gctcaccctc ttttagattc ttagatagga tataggctgc atttctatcc attgctctat
     3661 tcgcatcctc cagttccttt ctgcattttt caaataacgc accggctttt tctccagctt
     3721 cctccaaaac agctgctttt tccgtaagtt tccccagttc agcctcttgt cttgtaattt
     3781 tggatttcat tccatctaat tcgtttttcc tgaatcttaa aacatcataa acaacctgca
     3841 ttgacctgag cttatctatg tattttaaaa ccaggtttct atcttcgggc ttagacttct
     3901 gatattcaag tatttctgca tttaaatttt ccagatttct ctgggacaat ttgatttttt
     3961 cgcttatacc ggccaattcc tgttcaagac cggcaatagc cttatttaat tctgccgccc
     4021 tattctcagt ctcctttaca ttacctttca aagtctctac ttcattttca agcttcattc
     4081 catcctgtat ctgctgacga tattcaggtt cagtttttaa ggattccatt tccagcttaa
     4141 gctttccaag agcatgctct agattttcaa gttcacctgc actatttctt atattcacat
     4201 tattttttga tatggcacct ttcagcttgg ctgcagaggc caaaaatcca ttgatttttc
     4261 cttcaacgga ttttatttca ttccttatac ctaaagcatc cgcaagcctt gtccttaagc
     4321 caactagttt tggttgttct attactactt catttttaag gtattcatat ttctgtcgta
     4381 tctcactaag gttttcatcc acgtccggaa ggctgttcag gactatctcc agttgttttt
     4441 cagtctcttt aagcttttca ttcaattctc tgtttttaag gatcatctct aacaatccat
     4501 ctgcttttac agccttttca agctgcaccc ttttttctgc aaaaatctcc ttggaagata
     4561 aatgctgctg ttctttccta ttgataaatc caaactcctg gacaagcatc cagacctctt
     4621 tggcttcatt gtatttgctc tccagttgtt tccattcgtt ttcaaccctg ttcctttcag
     4681 atacagctgt ttccatcatc ttgtgagctt catcaagtgc ttcatcagta gcatccacat
     4741 atcccctcaa ttctccggat aaaacatcaa tcctgctttt aagcttggtc atcttcaggt
     4801 taagcttttc ctgcagctgc tttccgtatt cctcaaggta gaatatcctt tccagcattc
     4861 ctcttctttc tgagttgtta agaagtagaa attcctggaa actgttttgc ggcagcacaa
     4921 cagcacgggt aaagtcttca tggcttaatc ccagcaattc tttaatgtta ttgctcacat
     4981 cagtcgcctt atcacaaata ggaatttcac caacatcaga aatctggatg agacgtgcta
     5041 ttttaggctc acatgagttt gcactacctt tctttctctg gtacactctc tctactctat
     5101 aggtctttct ggtggagttt tttacaagtt caaatgtaaa agaaactttc accgtattca
     5161 tatttgcatt gataatgcct tgtgtacccc catcggctct cttcacctta ccatacaatg
     5221 caaaggtaat agcatccaga actgtagatt tgccgctccc ggttggcccg aatataccaa
     5281 acaaaccagt ttcacccagt gcttcaaagt ctatcctctg aatatcacaa aagctttgca
     5341 gtccttctat ctcaagaagc ttgggtctca tctgcttcag cctccttgcc atccttatca
     5401 ttatcattat cattgtcatt ataattataa ttatcattat aattatcgta atcattctta
     5461 tctttattgt catatgaagt tacatcatcg tacttatctg cttcaacata cttagcttca
     5521 tcatcgtact catctgcttc accatactta gcttcatcat cgtactcatc tgcttcacca
     5581 tacttagctt catcatcgta ctcccctgca tcatcttcct catcagtacc attcacaact
     5641 tcaataaaag ctcccatcag ttcgtccgaa atttctgttc ccatcctgta cttaaaatag
     5701 tctttgaaaa gctcgtcgat ttttttccct tccctgcttt gatagctgag aatctccata
     5761 tcttcagatt tcaatctggg acggatattg attatccccg gatgaagact tcttaaagct
     5821 ttttgctcat cggcagtcaa aaccctgtct gtcacaattt caagatcaat ccaggcattg
     5881 gcatctcttc catcttcaca ccacttaata gcttgttcta ttccttcctc agcaatccac
     5941 ctcctcaaag gtttaccgca gtctaagtaa agagggctga ttttagcctt ttctcccggg
     6001 attgcatcaa ttatatacac agctttgcta tgctctgctt cggaaaagct gtatgccagc
     6061 ggggaaccgg aataaaacac cggacatggc gcattgcgta tttcctgcgg acgatgcaga
     6121 tgcccaaggg caacaaaatg tgcattggaa ggcagtatgc caggatccac tgtcagtgct
     6181 cctcccacct gtagtgtcct ttcagattct gactccttcc cccctcttat gaaaaaatgg
     6241 cctgttgcca gatttactgt atcttctctg aatttcctgc tatgcaaagt aaatatactg
     6301 cctactttat ctgaatatgc tttttgtaga atttgttcat cagcttgttg tgaaagcact
     6361 tcttccaaac gagattccga aggataggga atagttatga taaccgcatg attttcacag
     6421 cctggcacac atagttccag ccaacccggc cccgaatctt ctattcttat attcccgctg
     6481 cttatcttgt acatacctgc gtcacttcct ggatatccca atagaattat gccgttttta
     6541 tatgccaagg gacttgcagc acacagacgt tcagggttat catggtttcc ggcaatcact
     6601 acaactgccc tggttccctt tccattcagc ctgtctattg catcataaaa aagttcctct
     6661 gctgctgcag aggggttata tgtatcataa atatcaccgg caacaagaac aaggtcaatt
     6721 ctttgttctt caactgtttt gcaaaggcag tctataaatt cccgctgctc atcaatacgg
     6781 tttacctgtt ccaaagtctt tcccagatgc cagtcagatg tatgtaatat tcgcatatta
     6841 tcctccagaa atttattaaa gagctctaaa ttaattataa tgctctcaaa ctcattaaaa
     6901 aagctttttt ttattactta atatttgctg ccaaatatcg taatcaggca catttcccaa
     6961 tctccacagt gaaaccccat ttattccaaa catcccggcc agtttaatct ttgcctgaac
     7021 acttcgggaa tcctcatacc atataacatt tttagtttcg tccccactat tataaaatgt
     7081 aatatatgga ttttggttta tgtttgaata attcatttca gcatctgcaa caagcttctg
     7141 tctgattgcc tcgtaactgg gtgaatatgg gtatcgattg ataaccttcc catcttttag
     7201 tttccattga actgcatcaa aagatatttg cagccatatc ttgtttaaat cttcaactcc
     7261 tgaatcctta tcggttattg cctttaaagc ataataaact tcatcaaaag gtgagagcgg
     7321 cgttatagta tatccattct gcatgtctgc atcagaaagc ttttttgcat aataatcatg
     7381 tgccataagt atgaccttat ctgcaatttc tccaatagct ttgtaatcgt aggcattata
     7441 gtagtcctga cccgacttcc ttttgggatg aactgcaaca tacaggattt tagaagtctt
     7501 atccagttcc tgtttcaatt ctgtcaaaaa cgaattaaag gattgtttta atagttcgcc
     7561 tttcatgctt tcaaaatcaa ttaccacacc atcaaatgaa atggtaatac catcttttgc
     7621 agttgcatta acctgattta ctattgattc tattacctgt cccctgattt cgggtttggt
     7681 caatacgtac tcaagcaagg gaatttcccc ttgatctata gcatttgata ccttgtcttc
     7741 atctccagtg ctcgaaatct tgtctttgtc ttcagcgctt gaaatcttgt ccttgtcttc
     7801 agtgcttgaa actattattt cattacttcc aatatctaaa actgtttcat tatctgcaaa
     7861 caccatcaat tgtgcagaaa ctttactttg gcgggccata ttatatggct gcaaaaatcc
     7921 ttcgggaaca gcatactcat tattattatt tctggtagta ttcaaaacaa cctgccggtt
     7981 tttctcatcg tattccaagc ggctccagcc aaagccaacc gaatcaaggt cattaatcat
     8041 atccatctga ttagccgatc gtattgcata aaaggcatgc agctcattta taggctgatt
     8101 aagcctatta tacatccgta tcatcatggc cgctgcctct tctctagtag ctgtattata
     8161 aggtttaaac ttattattgc ctgttccggt gataatgccg aaatctttgg caataattat
     8221 atattcttta tttttggaaa catcatcaaa tggactttcc atataggtta actgctttgt
     8281 caacatttca tagcccagag tatgtacaat cattactgcc atttcctctc gtgtgatagg
     8341 atcgtccggg cgaaaagcat tgtcattgtt taatattgtt gtattgtcat tgggcgttgt
     8401 acttggtata acaccgcttt ttaaggctgt ctctatatat ggataatacc atttgctgtt
     8461 gtccatatta tcgataaagc ttcctttttc cggctttacc aattcccatc ccattagtct
     8521 taccaaatag gcaacaaatt cacatctttt aattgtcagc cccaaaccga atttattatt
     8581 gcctataccg tcggtaatct ttagcaaacg caattcatgt atggcattat ccgcccaatg
     8641 cccctccggc acatcactaa aacggataac tgagttttca tctgcatgta tggggactaa
     8701 tggtattact aatacaataa ttgaaagtgt tatgataagc actgatctaa atggcttttt
     8761 tatcacatta ttcatattat tcttatttat aaataattca cctgctttcc agcttaattt
     8821 cctgcaatgg tttctcttaa attaaccttg agcggaatca aaaaccactc aattttaaca
     8881 ttaattatac acttcgtagg aaaaaatatc aaagctaaaa agcagttcga gtgttcgggt
     8941 gggtgcatat tttggagaac cgtcccctca tcaccctcat cagaaggtta ttggagaacc
     9001 gtcccctcat cagaagacta cattcctaaa gcccttccca ataatttctg aggtattggt
     9061 tattgtaacg aaggctttag gatctgcatt tccaatgtaa tccctcagct ttactgcttg
     9121 ctttcttgtc actattgtag taataacctc tttctcctga tttgtaaacg ctcctatagc
     9181 cttatatact gtggcacctc ttttaatgtt ttctgtaatg tattttttca cttgatcaga
     9241 attggcacta atgataacga cttgcttgca aaggttaagt tcttcaatta caaagtcaat
     9301 gataaacccc tttagaaaaa gtcccagaat tgaatacatt cctatttcta taccaaatac
     9361 cattcctgcc attacagtaa tgatgaaatc acctaacaaa agtgtctttc cgatatttat
     9421 gcgcatatat tttgccagaa tctttgcaat aatatctgtc cctcctgtag aggcattttt
     9481 attaaatact atggcagatc ctactgccgg caaaataaca gcatacacca actccaatat
     9541 tgtatctctt gtaagtggtt tgcttatagg ataaatcttc tcaagcagcc aaaccatccc
     9601 tgacaatgca aaactcgaat agatcgtttt gaatccaaaa ctaaagccta caaaaataat
     9661 tcctgcgatt gtcaataaga tatttatcgc catcataata agtcctacgg gtgatccagg
     9721 aaagaacctg cctactacta tagctatgcc gcttactccg ccgattgcaa aattattagg
     9781 tatcttgaac aaataaatac caagagcaac taaaaaaagc ccaaaattaa taattaaaaa
     9841 ctcattgaac cttttcaaat cactttcccc caaatcaaat attatataaa agctttataa
     9901 tttatcattc aattatcatt ccaattttca ttccaattag gatccgttat gccactatct
     9961 gaactgcaat tttctgtaaa ataattgaca ctaaaattcc ttatatcata atcaacatta
    10021 gaattcttta taatcgatat tagaattctt tatgtcataa ccagcgtcag gattctttcg
    10081 tcatgattca ttgtaattat tggaattctc tatactatct ttttcttccg tcttaagcag
    10141 tatatgattt tttaacagat tttcttttgc attccttata tgtttgcaaa ttaatgcttc
    10201 agccttgtcc acattctctt caatgattgt ttcaagaatt tccttatgtt cttttagtgc
    10261 ttcttttgct cgtcccggtg tttgaagaga ggtctttctg gctctctgaa cgtaacggtg
    10321 aaaatcactg agcatatgct tcagtggtct gctcttgcag gcatcatata tgatcctgtg
    10381 gaactcagta tccaggtcct gaagtcgtgt acaatgcttc ttatttatgt aaaactccgc
    10441 cagttctacc tgctccttta gtttttctat ttcatcaggt gttatcttct gaattgccca
    10501 acgcactgca agtttttcga tctccatccg tatgttgtat atatcttcaa tatcctcatc
    10561 agttacccct aaaaccatag cccctttatt agttacaatt tttaccagac cttcgcgttc
    10621 caattgtctt attgcttcac gaataggtgt acgactcact ccaaactgcc ttgataattt
    10681 tgtttcaact aaataatcac cgggctgata ctctccgttt aatatacttt cttctaattg
    10741 ccctaaaact ttgtatctta atgaataccg ggcatttcca tttgatactt ccatattcat
    10801 acagcatctc ccttaagtgc attactgata ttgctgatat cgctaatatt tctaatattt
    10861 ctaatattgc taatattact aatattattt ctatgatttt cagccatatc cattattata
    10921 cttcaatcaa tacaattcaa cacatataag aaatatactt cgattcatgc atgctaatgt
    10981 acatgtatac aggctaataa atttgattat caattctaat aggttttggg ggtacattta
    11041 ctaaaagtga agaaaaattt accaggaact gtcgttacgc tgcttaccaa tattttcttc
    11101 acttaaatta agtgcaccca ggttttggag aactgtcccc taaaataaaa ttgagaactg
    11161 tccccatttt ggagaactgc ccacctaaaa agccccccta aaaaaaataa aagatatagt
    11221 tcacagatgc aaactatatc ttcttaagtg taataagcat gttggaaggt tctaaagtat
    11281 tcttgcaaca gctcgtgcca ttgaactgtc agattttatt tttagtggta aagctatgac
    11341 ctcgaagttc tctacaccta gtaatttatc aagattagtt agattctcaa gtataaatat
    11401 ttcgtttgca aaaagtattt tatgaatttc aaaaggatat ttatctggag aaggcatatc
    11461 catacccagc atttttatat ttttctctaa taaaaatcta catatatcaa taccaacaac
    11521 aggatgctcc tcgtaatatt ttgtcatacc ataatattta tcctgtcccg tataaaataa
    11581 cacaatgcta ttatccttta ttaatttctc atattccgct ttcattttta ttacagcttg
    11641 atttcttacg tccaggacac atccagttgc aatgaatggc tccagtgacg cttgagatat
    11701 atattcatta ctttctgtta gatgcatagg tgaatctata tgagtacccg aatgcatact
    11761 aatatcaagc ctgtggtcat tatacttatt tacgcttaag tattttatct ggaataagtt
    11821 tgttcttata tccccgggat aaaccggcat attatcttcg attgtatgcg ataaatcaat
    11881 taaacttgtc aaatgcccac cttcgttcta ttttctattt tgctattata gtatctaacc
    11941 tgaattcatt ataacccata atagggcaca ttttatttat ttccgcacat actttttgta
    12001 agtaaataat aggattgata caacataagc ttgtattact accatcagta tctcctgtat
    12061 tactcttggt atccataata cccaaaagcc cattttcccc agtgcaggta taaagattcg
    12121 caaaatatac gtatttacag tagtaactat tattcctgaa acaccggtgg acattaatat
    12181 tttaaaaaag tcattgtaca attgtgaatt attatatatc tttctaaata tttgatcggc
    12241 taaaataaat actgatccga ataaacatgc aactatcaat ccaatagtcg taaaatctct
    12301 atattttcca atgctattta taatctgggc ccatggggaa gcagggatta aagcaacatg
    12361 aatgtagttt actattccca gcacaccaat aattatgaaa aataataaaa aggatacctg
    12421 caatctttta gcattaactc ttctcagcat tttccacact aatccggtaa taactccgcc
    12481 agatacggca gttaccgtaa gccatggaat ataagcacct tctggtttta tgataaatcc
    12541 ggtaatatct acaagtcctg atgcaattcc accaaataat ggtccaaata gaattgcagt
    12601 aattttcgta aacactcctg aaaagctaat gcgcattccc ggagctccac ctaaatagac
    12661 catataacta aaatttctta ctactattgc gagagcaatc aataagcttg tttgaacaac
    12721 atttctaata tgattctctt catttttcaa aaacataaat tatccctcac ttcaataata
    12781 tgatttgcat tcaacagaaa atagccatca catcatcgaa atagaaagac atcttatatg
    12841 taacagcgga tgtaatatta tgtcgcaaac cctcaatttt tagataggtt ttcattttag
    12901 agactctact cccattctct aactctttac gcataatacc tgctctgttc aacacacaca
    12961 atattttatt cagattagat tataccactt ttgtaaaatg aagactattg gttttttgca
    13021 taaaattttt acatcattaa gacaacttta tggttttcca cttgcctgaa ttatatcgga
    13081 ttagtattag caaagaacgg accagctggt cggcaaccaa agcataccat gcaccttcca
    13141 agccccatcc aaaaacgtga atacctaaca aagcaagtcc gggacgaacc aatagaacag
    13201 ttataaatgt aacaacagct gtggcttttg tatctcccgc tcctctaaga gcaccagcaa
    13261 gaataaactg ggaagattga aatggctgga ttagagccac taacattaat atttttgcgc
    13321 cttgatgtat aactgcagaa tcatcactat ataaagctac aataggtttg gcgaagaaga
    13381 aaaagatcaa cccgagcaat atagaaacag tcatgccaat acgtctcgta cgactgcaat
    13441 atgcttgtgc catatcaggc cgttttttcc ccagactctg ccccataagc gacgttgcag
    13501 atactgcaaa ggcctggcca ttcataaagg acatagcctg aatattcata catacctgat
    13561 gtgttgcaaa tgcaacagtt ccaagagaag caacggttat tgaatagatt atcattcctg
    13621 ctcgcatgac aatttgttct atcaatgcag gaatgcctat tataaatatg ttcttcatag
    13681 actcccaatg aggtttaaac ccatctttta aacgcagacg cagatattgg tttccacgta
    13741 gaatagcatt tactgctaat atgaaagcca cacactgtcc aattactgtg gctaatgaag
    13801 cacctgcaac ttccatcttt ggaaatccta aatggccata aatcatcaaa taattaaata
    13861 ctacattaac cccatttgca gtcagattat atatcattgc tgttctagaa tcgcctattc
    13921 ctcttaaagc tgctgttatg gtgctggtta gtgccataac aacaaatcct gccatctgaa
    13981 tttttagata aatagttcct ccggctaagg tttcagcgtc agctgctccc ataaaacgaa
    14041 ttaatggttc cgagtatata tatccaatta tagatgatat aaccgataga acgaaagtaa
    14101 gcaataatga ctgtcgaaga atagtgtttg ccttgttata atcacctgag cctttatacc
    14161 ttgctactaa tgcagtagcg cctacattca tagccataaa cattgtcatc ataaggaatt
    14221 tcggctgatt ggtcaatcct acggcagaaa ttgcccatgg acctaattga cctaccatca
    14281 tcatgtctac catggatgta agctgtgtaa gggttaattc aacaaaagac ggccacgcaa
    14341 tccttactat atctttatat agcatcctgg aacttacttc ggacggtaga tgtttgctta
    14401 cccgttcctg ttttccgtgg ctatatgcat catcattacc aggttccacc agatcaaggg
    14461 ccatctggat tttacctgtt ttcacttcag ctatttgagc ttcaggtatt ttgatttcac
    14521 ctgtttggat ttcagatatt tgaacctcac ctgcttggat ttcagatatt tgaacctcac
    14581 ctgcctggat ttcaaatatt tgaacctcac ctgcctggat ttctggaatt tgaattttag
    14641 tcatttggac ttcagctgct tgaacctcaa tacattcatt gccttcattg tcttcattgc
    14701 caggttcatc tataatatta atattctcaa attgagtctt accttctatt tgtgtcttca
    14761 actttattga tacacatcct ttatcaacac aaatcccact tagtgttcaa attattatta
    14821 tcataccatg gagctaaagg aatgtctaca atgatttaat tttttaaaac ctgcattttt
    14881 aaatcttcca ttttccagga taacaatggt atcactgctc attatagtgc atagcacatc
    14941 cttcacaaaa agaagccata ttttggactt aggatgcatt ttcttcctaa tatataatac
    15001 ttaattttta ttatttattt caattccatc aacagttact gattgaacct ttgagacgtc
    15061 caatggcaga gaagatgtaa atttaatttt tacttttcca ttatcactaa agcttctcac
    15121 cgactcaaaa gtttgaacac tgccatcagt catattggcg ctaacagtta ttgattgcgg
    15181 ctctttgttt tcaccactgc cggtcaatgt tattcccatt ggtgatacag aaatactagt
    15241 tacagtccac gtatcaaact taatattaca atcagtttgc ttttgctgcc ctacagactg
    15301 aattttaaat gcaacttccc agtttcctgt tgtgtaattg ccattggata caaaaaaccc
    15361 catcaatttt atttcattaa gattcaagtt atttacatca aagatgtatt cgacataatc
    15421 attgccatat ttggtatccc ctgattcatc tgttccgaaa tatatgttgg atgcactaat
    15481 atttatcttg tcacctgcag tattaacaaa atagaaatat ccatggtcat caataccctc
    15541 tcccacccat ttagtttgaa tgtgaaggcg gccatctata taaccaatat tagaaatata
    15601 cataaattct atcccgggaa gactatgttt catttgatct gcctttaaaa cttttactat
    15661 gccttgttct ttaaactctt cgaataaaga gcctcctccc cccggaatat tgttcatatc
    15721 aagaggaaca gtttgtgaaa cgttggctga tgctatgtct aaaaggttga tacccgtctc
    15781 aactgcatca aatattaatt tgtcacttaa aaatgaatca atgcgaaact ttactttctt
    15841 tccgtctaat ttttctccac cgttagcctg catacgcaag gttgcagttt tcgttatttc
    15901 atcgtaattg acaatttggc aattaaatgc gtgcattcca gttaatgaat agtcgtaaat
    15961 atcaagagtt ttatctattc tattgcctgt taaatcctgc atggtcagat aaataactgc
    16021 catttcatca tcattcatgg ctgccactac ttccatttta attccattgt cttcactgct
    16081 gatttccaca ggttgaagca ttagtgcaat atcagaggca acaaaaaaca aaagcttttt
    16141 aaaatttgga atggccgcag ccactaaacc cacagagaac ataaggcaca aacaaactgc
    16201 caatactgct gcaacaattc ttttaaagtt catatgagat ttttgctctt taattttttt
    16261 atctacctcc ggaataatgt tatattcagg tatctcaaca gtgcttagtg cattttttat
    16321 aatattttca tcatactcat aattcatttt ctttcctcca atcgcgcata atatgaatta
    16381 gtttccttca gtgctttcaa caactttgtt tttgcccgtt cataacgttt tcgtagtgtt
    16441 gtggcagaag cattatagat agcttctaag tctgcataac ttttttcatc aataacccgg
    16501 ctaaacacca gggcacgttc tgccggtgac agtgtcataa gcgcctcttt taaatcatcg
    16561 cttataaact ttgagtctgt attcgtttgc tgaacaaaga acaataattt cttttttctc
    16621 agagaatcaa tacaggtagt atatgcaata cgatataacc atgctgacag agatgtcccg
    16681 tttttaaagc tttttctttt attataagct ttgataaatg ttatttgcac agcatcctga
    16741 gcttcaaagt agtcacaaag tatgttatga caatatctta gcagaggttg tccatatagt
    16801 tctatagcac agtgcagttc atactctccg cctgcagtaa atgcataatc aagttccctt
    16861 gcttccatcc ccccgctccc ttcgttaatc tctattcatt agtatctatt cattagtgcc
    16921 tattcattag tatctattca ttaaactata tcttaactaa actctccctt caatatttaa
    16981 aatcattttc ccgtaaatta gatttgcgcg caaaataaaa aacagttttc acataaatta
    17041 aaacgatttc tacttatata acgtaagcaa atatcaattt gtgacatgaa attaaaatta
    17101 atcaaacgaa aatcagaatg aatcaaatca ccataactaa ttttatatta agatatgcag
    17161 cattaataat gccaataatt gttacagcag catcaatagt tatatatttg gaatttgcca
    17221 gtctatttcc gtttgcccag tctctctcaa aaagttattg gcttttgaga aatgcttgca
    17281 gccgaaaaac cctctattag ctgaaagcgg gctgggatgc gcagaagtta agatcaaatg
    17341 tttttgattt gttatatatt ttgctttatc tcttgcatta tttccccaca ataaaaatac
    17401 tataggggta tcttttttat ttagcaattg tattattgaa tctgtaaaaa cttcccaccc
    17461 tttgtttcta tgggaattag cagcatcagc tacaaccgtt aagcttgtat taagaagcag
    17521 cacgccctgc cttgcccacg gcactaagca tccgttattg ggaatatagc agtttaaatc
    17581 gttaaccagt tccttaaaaa tattaagcaa agatggtgga atatcaactc ctttttgaac
    17641 ggaaaaagcc aatccatgag cttggttttc tccatgatag ggatcctgtc ctataattgc
    17701 tactttaaca tctttatatg ttgttaattt caaagcttca aaaatattat ctttatcagg
    17761 gtatatgatc tttgtactat
//
LOCUS       DAOSDD010000073        17743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1291_length_17743_cov_4.988410, whole genome shotgun sequence.
ACCESSION   DAOSDD010000073 DAOSDD010000000
VERSION     DAOSDD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17743
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_17743_cov_4.988410"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..375)
                     /locus_tag="PK733_08285"
     CDS             complement(148..375)
                     /locus_tag="PK733_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10576.1"
                     /translation="MENSSFYPDVSGYYLKNALKIINECGVEIVDIKITAPPRCRSKD
                     YDDDFRVLRIRSVGPNKVELVVCNPYILANS"
     gene            complement(378..935)
                     /gene="frr"
                     /locus_tag="PK733_08290"
     CDS             complement(378..935)
                     /gene="frr"
                     /locus_tag="PK733_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOJ10577.1"
                     /translation="MYKDELKQTEEKMKKSINVLKQELSYLRAGRANPAILDKVLVDY
                     YGTLTPINQIANIAVPEARVILIQPWDARSLKDIEKAIQKSDIGINPSNDGKVIRLVF
                     PVLTEERRKELTKVVKKTGEENKVAIRSIRRDSIEKFRALKKDGEMTEDDLRNAEKEV
                     QTLTDKYIEETDKIVEAKEKEILEI"
     gene            complement(953..1660)
                     /gene="pyrH"
                     /locus_tag="PK733_08295"
     CDS             complement(953..1660)
                     /gene="pyrH"
                     /locus_tag="PK733_08295"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ10578.1"
                     /translation="MENIKYKRVILKISGEALAGSQGFGLNTETLDGITESIKEAFNL
                     GVEIAIVVGGGNFWRGRSGKGMDRITADHMGMLATTINALALQDSLEAKGIPVRVQTA
                     IEMRAIAEPYIRRRAIRHLEKKRIVVFACGTGNPYFSTDTAAALRAAEIDAEVILLAK
                     KVDGVYDSDPVKNPNAKRYNKLSHLDVINQRLGVMDSTATSLCMDNEIPIIVFGLDKP
                     ENIVKVILGEDIGTSIT"
     gene            complement(1775..2419)
                     /gene="tsf"
                     /locus_tag="PK733_08300"
     CDS             complement(1775..2419)
                     /gene="tsf"
                     /locus_tag="PK733_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ10579.1"
                     /translation="MITADMVKQLRERTGAGMMDCKKALVETDGNIEKAVEYLREKGL
                     AAAAKKSGRIAAEGLVESYIHGDGRIGVLVEVNIETDFAAKNEEFRMFVKDIAMQIAA
                     SKPEYIRKEDVPAEIVEKEKEILKVQARNEGKPEKVIEKMVEGRLEKFYKEICLLEQP
                     WIKDPDKTINQLLVEKIATIGENINIRRFARFERGEGIEKKQDSFADEVMKQIG"
     gene            complement(2499..3353)
                     /gene="rpsB"
                     /locus_tag="PK733_08305"
     CDS             complement(2499..3353)
                     /gene="rpsB"
                     /locus_tag="PK733_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOJ10580.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTVKKVEEAYLFVREAAMNGEDVLFVGTKKQAQDSIKEEAERCGQYYVNERWLGGMLT
                     NFKTIRRRIERLNELVKMEEEGIFDVLPKKEVIKLKGEMEKLEKNLGGIKDMKRIPGA
                     IFVVDPRKERIAVLEARKLGIPIVAIVDTNCDPDEVDYVIPGNDDAIRAVKLIAGKVA
                     DAVLEGRQGEQLTDTNSVNTSYNNNADEDIIQEPSSSDLTSDLTSDLTMEAAEAADDK
                     TDETATEE"
     gene            3806..4894
                     /gene="ytvI"
                     /locus_tag="PK733_08310"
     CDS             3806..4894
                     /gene="ytvI"
                     /locus_tag="PK733_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOJ10581.1"
                     /translation="MANLSNIKRARLLKIGLALLGVIVGVFLIYKLSVYITPFIIAFV
                     IASIIEPIIRFFMRRAKMPRKVAAPIVLTFFIVSFGLIITLIIVRLIDEMKSVATVLP
                     KIVPDIYAYLNMLLNKGIDIFEWLPPEITDNIGNLVAKLSESLSNILNSIIKGAYITA
                     VSIPEALVFTVITIVSTYFMSSDRERISKFFSSQFPEKWINKGKSIIENMFSALIGYI
                     KAQLILMMITFTELFIGFTLIRVRYSLLLAFIISFLDALPILGTGGFLTPWAIYSFLT
                     GNIRLGISLLILYGVVLIVRQSIEPRILGQQIGVYPLVTLIAMYSGLKLFGFIGFILG
                     PILVLMFKNILSGILKNRSIKDILEHQS"
     gene            4994..5704
                     /gene="sigK"
                     /locus_tag="PK733_08315"
     CDS             4994..5704
                     /gene="sigK"
                     /locus_tag="PK733_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOJ10582.1"
                     /translation="MFTGIFSAIFETLSNACFLLGYVSNPNSFPQPLTPEEEARFLEE
                     YKNGSDDARNILIERNLRLVAHIVKKYSNNCEDTDDLISIGTIGLIKAISTFDNEKGA
                     RLATYAARCIENEILMQLRYTKKIQNEVYLQDPIGVDRDGNEISLIDIIENDNESIVD
                     KVELKMQVKKLYDIMKSALKNREKIVLELRYGLCNGTSKTQREIAKMLGISRSYVSRI
                     EKKAIKKLTRELKPENCN"
     gene            complement(5786..7444)
                     /locus_tag="PK733_08320"
     CDS             complement(5786..7444)
                     /locus_tag="PK733_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ10583.1"
                     /translation="MEAKRLIAVLLIFSAIFIGLGYRFFNLQIVQGEKLSRLASAQRI
                     SSLEIEKLRGDILDRNEIPFTNRIQKYLVLLKPLLLQGHENDLKNICNILEIDFENVM
                     RDIAFSRKPYVVEADEKKKNIINDLKHEGISIINSLKRYDDTTLARHILGYVNKIDGA
                     GEAGLEKFYNDALEYDTESFVGVITDGKNNVLPGMGYRLMKVQGRNKKLNVKLTLDYH
                     IQKIVEDVMDKHNITGAVVVEEVDSGNITAIASKPDFDQYNIGNYLNSTGKELFNRAV
                     ASYNLGSIFKIIDAAAMFELKEHWDEEYFCTGSIKVGDREFKCYSYDKGGHGYVDFDK
                     AFALSCNTYFIDAAIKMDKKSLLEKARRFGLGNITGIREQGIDESGGNIPSLDGWYSD
                     GDIANISIGQGAIMATPLQVADIVATVANGGIKNSVNIVDSIVDDYGNKVREVKRKQG
                     HRVIEKDIADRIKNLMEKVIDEGTGTMVDINEYGGAAGKTGSAETGQYIEGQRVVHAW
                     FAGYFPRKNPRYSIAVFVENGRMGGSAAGPVFEEIAKKIMEKRF"
     gene            7666..8904
                     /locus_tag="PK733_08325"
     CDS             7666..8904
                     /locus_tag="PK733_08325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ10584.1"
                     /translation="MDRTILHCDLNNFYASVECLYNPDIRDKPVAVCGSQSTRHGIVL
                     AKNYIAKKYGVITGEPAWEAKRKCPGLVTVSPNYLLYLRFSTNAINIYRRYTDLIEPF
                     GIDECWLDVTDSIRLFGSGSDIAYKIKGQIKEELGVTASVGVSYNKIFAKLGSDLKKP
                     DAVTEITSRNFKDTLWPLPVRELMYVGKSTEAMLKKAGIYTIGNLACTPPAFLIRHLG
                     KWGESLWVFANGYDDLSVSKADTGPLIKGIGNSLTTPRDLTCNEDAKILFYVLSESVA
                     LRLRKNNLKGKTVQITIRDSNMVSIERQGQLKLHTFISSEIAEKAMEIFAYSWNWSNN
                     IRLLGVRVTDLVSADSYVQLSFFNDDKRAKKQLLEQSIDKIRSRFGYYSVQRALLIKD
                     SNLNANPAEDNVIHPVSYIR"
     gene            8921..9157
                     /locus_tag="PK733_08330"
     CDS             8921..9157
                     /locus_tag="PK733_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10585.1"
                     /translation="MKKVFVEVIVKYLCDGTKIPLSITWENGKEYIIDKVTDIRRAAS
                     LKAGGQGVRYKCRILGKERYLWFEDDGRWFVEGK"
     gene            complement(9186..10427)
                     /locus_tag="PK733_08335"
     CDS             complement(9186..10427)
                     /locus_tag="PK733_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ10586.1"
                     /translation="MDKIELLAPAGNLEKLKMAIIYGADAVYLGGEAYGLRAYADNFS
                     LEEMKEGIEYAHSRGKRVYVTMNIIPHNSDLIGIQDYAAQMQRIGADALILSDPGIFM
                     LVKEAAPEMEIHLSTQANNTNWASAQFWYEHGIKRIILARELSLKEIGEIRHKVNKKL
                     ELEIFIHGAMCISYSGRCLLSNYMAGRDSNKGLCAHPCRWKYFLMEEKRPGEYMPVFE
                     NESGTFIFNSKDLCMIEHIPEIIQAGVTSVKIEGRMKSSYYVATVVRAYRKVIDSYCN
                     DPESYRFDPMWMEEISKASHREYTTGFYFKKPGAEEQVYNTSSYIRDYDFVGLVRNYD
                     KATGIATVEQRNRMFNGEEIEVVIPGGEYFTQVIESMKNEDGENIDSAPHPQMTVYMP
                     MAREVAEYAILRRKPQYIKPA"
     gene            complement(10420..11073)
                     /locus_tag="PK733_08340"
     CDS             complement(10420..11073)
                     /locus_tag="PK733_08340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOJ10587.1"
                     /translation="MICYDYINKYIRDTIKKDEGILLELRNYALLNNVPIVHPEVARL
                     LVVLGHMVKPSKVLEIGTAIGYSSILMSQILNQGGIIDTIESNDAMIDLAKENIKIAG
                     CKDVINIIAGDAAEVLPCLDKKYDFIFLDAAKGQYPEFLPHCIRMLNNNGVLISDNVL
                     YKGMVANEELVVRRKKTIVKRLKDYLDTICSDNSLETCILPVGDGVAVSFKRGDNIG"
     gene            complement(11208..12302)
                     /gene="mltG"
                     /locus_tag="PK733_08345"
     CDS             complement(11208..12302)
                     /gene="mltG"
                     /locus_tag="PK733_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOJ10588.1"
                     /translation="MKKSIIRWSIAVLMLAIVFGCGYATYSYIVKNYSAVSTIQQILL
                     EDQLLIEIPTGSGTEAIANILNKEGVIKNAFIFRMLSRVNGYDGTYRSGVHAIDKNTN
                     YNSLKGYDILMEIMSGSPLNFVGVRVMIPEGYNNSQIIELLEKANLANKKNFIAAEKS
                     VKFDFKFLKNLPERENRLEGYLFPDTYEFDPKSGEKEIIHKMLKNFDTKFKPEYYIRA
                     EELNMSVDDIIILASIIEREAKVAEERPIIAGVFYNRLRSKNGSLRKLQSCATVQYVL
                     YKREGKMKEIISIEDEKIEDPYNTYLYEGLPPGPICNPGQESIEAALYPKEHDYLYFA
                     LKRDGTGEHYFTKTYTEHLGAQLKAQLNAK"
     gene            complement(12353..14170)
                     /gene="typA"
                     /locus_tag="PK733_08350"
     CDS             complement(12353..14170)
                     /gene="typA"
                     /locus_tag="PK733_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOJ10589.1"
                     /translation="MKVRDDIRNVAIIAHVDHGKTTLVDGMLRQSGIFRENERLQERV
                     MDSNDLERERGITILAKNTAVVYKGVRINIIDTPGHADFGGEVERTLKMVDGVLLLVD
                     AFDGPMPQTRFVLKKALQMNLKPIVVINKIDRPGARPLEVVDQVLELFIELGADDGQI
                     DFPIVYAAARDGIAILDLNDERVDLEPLFKVILDKVPAPKGDTEGSLQMLVSSIDYNE
                     YTGRVAIGRIERGKIKWGQQAVICKKDGTKQESKLSRVYIFEGLKKIECSEAAVGDIV
                     AVPGLEGINIGETICDLDNPEPLPFINIDEPTVSMTFLVNNSPFAGREGTYVTSRHLR
                     DRLFRELENDVSLRVEETDTPDAFNISGRGELHLSILIETMRRQGYEFQVSKPRVIMK
                     EIEGVLHEPIEYLLIDVQEDYMGMVMEKIGPRKAEMVNMHSANEGYMRLEFKIPARGL
                     IGYRSEFLTDTKGNGIMNHVFYGYEPYKGDLQVRPRGSIIAWEEGESITYGLYNAQER
                     GTLFIGPGVKVYEGMVVGENSRPEDIVVNVCKKKHVTNMRASGSDEALRLTTPKVLSL
                     EQALEFISDDELVEITPKNIRLRKKILDTEQRAKARSKG"
     gene            complement(14333..16717)
                     /locus_tag="PK733_08355"
     CDS             complement(14333..16717)
                     /locus_tag="PK733_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOJ10590.1"
                     /translation="MNEKTLKVLEFDKIINQLAGFTASQLGREIAESLMPSNDMTVVA
                     DRLRETDDGVSFILRRGNPSLGGIRDIRDSLKRVDMGAVLNMGDLLRVADVLRLCRNL
                     KSHAAGDRKVDEENNVIFGFIDCLEANKRIEDKISYSIVSEEEMADDASPTLNSIRRQ
                     IRERQESIKEKLNGIIKSPHYQKYMQDPIVTIRAGRYVVPVKQEYRNEVPGLVHDSSA
                     SGATMFIEPMAVVEANNSIKQLQIKEQAEIERILAELTEDVAGILPQLKTDMSILAKL
                     DFILAKANLSLKYNGVSPKLNTDGCIMIRKGRHPLLNAETVVPIDFWVGERFTSLIVT
                     GPNTGGKTVTLKTVGLFALMTQAGLNIPAGDNTEICVFNKIFADIGDEQSIEQSLSTF
                     SSHMTNIVKILENADSQSLVLLDELGAGTDPTEGAALAMAILEELHEKRAITVATTHY
                     SELKIYAMTSEHVENASCEFDVETLRPTYRLLIGVPGKSNAFAISKRLGLQEEVLDRA
                     RDYLTQEDIRFEDVLLSMEKDRTKAETERVKAEALRLEIEKLKNELEEQKRKISSEKD
                     KIIREAREESKRIITGAKAEAEAVIEEVKRIEKDRQNVHKSRALEEIKQKLRNKVNKI
                     DESLVETIMPRQSYVKPPENLKPGDTVLIVNLGQKGIVIEPPGANGEAIIQAGIMKIN
                     VHVTNLKLVDEQKIEIEKVGAGEIGRAKAMNISTEIDLRGFNLDDAIDSVDKYLDDAS
                     MTGLKSVTLIHGKGTGILRAGIHRHLRTHPHVQSFRLGKFGEGETGVTVVELKS"
     gene            complement(16895..>17743)
                     /locus_tag="PK733_08360"
     CDS             complement(16895..>17743)
                     /locus_tag="PK733_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ10591.1"
                     /translation="FRSVFEQIGPGLRLAVSGAAPLDPDVVIGFEKIGLYLLQGYGLT
                     ETSPVICANNDFASKPGTVGHPLGGIEVAIDSPDENGMGEIITRGGNVMLGYYEDPDT
                     TAQVMYKDGWFRTGDLGIVDDNGFLKITGRAKTMIVFTNGKKAFPEEYEVLLNNIPGV
                     KDSFVWGNRAFDGGIQVCAKIVVDKENLPALTEDTHSKFADDTTLETKLAAYFNNAIK
                     EINKNVPQYKIIRYFVMTYEDLIKTTTLKIKRHIEYENTMKVLDKSGLGMRKASGLLI
                     DELKYR"
BASE COUNT         5019 a         3799 c         2715 g         6210 t
ORIGIN      
        1 tcctcatttg tgaggatatt atatcatgaa tttcgaagaa attgcatgat ataatcgtgc
       61 atgtgcgttt tttgcgaagc aaaaatattc gcacgcacat aaattccgcc ggaggcatat
      121 aatatcctca tttgtgagga tattatatca tgaatttgct aaaatgtaag gattgcacac
      181 aacaagctca accttgtttg gtccaacact gcgtatcctc agtactctga aatcatcgtc
      241 ataatcttta ctcctgcacc ttggcggtgc tgttattttt atatcgacaa tttcaacacc
      301 acattcattt attattttta atgcattttt caagtaatac cctgaaacat cgggataaaa
      361 gctgctattc tccatattta aatttcaagt atttcctttt cctttgcttc tactattttg
      421 tcagtttctt caatatattt gtcagtcagt gtctggactt ccttttcagc atttcttagg
      481 tcatcttcgg tcatttcgcc atctttctta agggctctga atttttcaat tgaatctctc
      541 cttatagacc ttatagcaac cttgttttcc tctcctgtct tcttaacaac ctttgtaagt
      601 tctttccttc tctcctcggt aagaactggg aatactaatc tgataacttt tccatcatta
      661 ctgggattaa ttccaatatc agatttctga attgcctttt caatatcctt taaactcctg
      721 gcatcccaag gttgaatcaa tataactctg gcttccggca cagcaatatt ggctatttgg
      781 tttatgggag ttagcgttcc gtaataatca accagcactt tatccagtat ggccggattt
      841 gctctgcccg ctcttaaata agacagttcc tgttttaaaa cgtttatgga ttttttcatc
      901 ttttcttccg tttgtttcaa ttcatcttta tacatgaaaa tcctccttat cgttatgtaa
      961 ttgaagttcc tatatcctca cccaggataa ctttaacaat attttcaggt ttgtcaagtc
     1021 caaagactat tattgggatt tcattatcca tacataatga agtagctgtg gaatccatca
     1081 ccccaagcct ctgattaata acatcaaggt gggataactt attatacctt tttgcattgg
     1141 gattcttaac agggtcacta tcatacaccc catccacttt tttggcaagt aaaattactt
     1201 ctgcgtcaat ttctgcggct cttaatgcag ctgcagtatc tgttgaaaaa taaggattcc
     1261 ctgtaccaca tgcaaacact acaatcctct ttttttccag atgccttata gctcttcttc
     1321 gtatataggg ttcagctatt gctctcattt caattgcagt ttgaaccctt acaggtatgc
     1381 cttttgcttc taatgaatcc tgaagagcaa gtgcattaat tgttgttgcc agcatgccca
     1441 tatgatctgc tgtaatgcga tccattcctt tgccgcttct tccacgccag aaattgcctc
     1501 ctcccactac aattgctatt tccactccca gattaaatgc ttctttaata ctttcagtaa
     1561 taccatcaag tgtttcagtg ttaagtccaa aaccttgtga acctgccaga gcctccccgc
     1621 ttattttcag tatcaccctt ttatatttga tgttttccat tgatgttatt cccccgccct
     1681 tttaaccatt ataccatatg gtataattaa tataatattt attactctaa aaaaggaaca
     1741 tataacaagg tatatgttcc tttttttaat tcttttaacc tatttgcttc ataacttcat
     1801 cagcaaagct gtcttgcttc ttttcaattc cttctccacg ttcaaacctt gcgaatcttc
     1861 ttatgtttat attttcaccg atagtagcaa ttttctcaac aagaagctga tttattgttt
     1921 tatcaggatc ttttatccat ggctgttcca aaaggcaaat ttccttataa aacttttcca
     1981 atcttccctc aaccattttt tcaataactt tttcaggttt gccttcattg cgagcctgca
     2041 cttttaaaat ttccttttct ttttcgacga tttctgcagg tacatcttct tttcttatat
     2101 attccggttt acttgcagca atctgcattg ctatatcctt tacaaacatt ctgaattctt
     2161 cattcttcgc tgcaaaatcc gtttcaatgt taacctcaac taatacacct attctgccat
     2221 ccccgtgaat ataggattct acaagacctt cagcagctat ccttcctgat ttctttgctg
     2281 ctgctgcaag tcccttctct cttaaatact caacagcttt ttctatgttt ccatctgttt
     2341 ctacaagggc ctttttacaa tccatcattc ctgccccagt tctctcacga agctgtttaa
     2401 ccatatcagc agtaatcatt aaaactcctc caaacccata tttatatttt ttaagcattt
     2461 aatcattttc ttcaagccat tcactttttc tgaagttatt actcttctgt agctgtttca
     2521 tctgttttat catctgcagc ctcggctgct tccatagtta ggtcgctagt taggtcacta
     2581 gttaggtcac tacttgaagg ttcttgtatt atatcttcat cagcattatt attatatgat
     2641 gtattaacac tgtttgtatc agtcaattgt tcgccctgtc ttccttccaa tacggcatca
     2701 gctactttcc ctgcaatcaa cttaacagcc cttattgcat cgtcatttcc tggaattaca
     2761 taatctactt catcaggatc acaattagta tcaactattg caactattgg tatgcccaat
     2821 ttccttgcct ctaaaacagc aattctctct ttccttgggt caacaacaaa aatggctcct
     2881 ggaattctct tcatgtcttt tataccgccg aggttttttt ccagcttttc catttcgcct
     2941 tttaatttta ttacttcttt tttaggcaaa acatcaaata taccctcttc ttccatcttt
     3001 acaagttcgt tgagacgttc tattctgcgg cgtatggtct tgaagtttgt aagcatacca
     3061 ccaagccatc tttcatttac atagtattgt ccgcatcttt cagcctcttc cttaatggaa
     3121 tcctgtgctt gtttttttgt tccgacaaaa agaacatctt ccccgttcat agctgcttct
     3181 ctgacaaata gataagcttc ttcaaccttt tttactgttt tctgaaggtc aataatataa
     3241 ataccgtttc tttcggtaaa tatatattcg gccattttgg gattccatct tctggtctga
     3301 tgtccaaaat gtactcctgc ttccagcagt tgtttcatag aaattactga catataaaat
     3361 cctcctaaat tttgttttat acctccacag cagttaactc catagaagac catggactga
     3421 accctattgc tcaaagattt ttcattctat ggcacccttc cataaatcgt actgtgtgtg
     3481 tatttttcgc tatgtagtat agcacatgaa aaacttttta tcaactgttt ttgcaaattt
     3541 cttggaattc tctgattgat ttatgataat gtctcaatat accacttttg attatgtccc
     3601 aattacatga aaaatatagt ataataaaat cgtatcattg caatataaca gattaggtgg
     3661 gaaaaatgag aatggtgaat ttaacatgac tgaagaaaat atagaaaatg caccccattt
     3721 gtattttaat gttgtgcaat tcatttacga tgctttataa taaaaatata tacataaaaa
     3781 gaaaggtgtt gtgagactga aatatatggc taacttaagt aatataaaaa gagcaaggct
     3841 tcttaaaata gggctggctt tattaggagt aatagtgggt gtttttctta tatacaagct
     3901 cagcgtctat ataacaccgt ttataattgc atttgttatt gcatccatta tagaacctat
     3961 cataagattt tttatgagaa gagctaaaat gccccggaaa gttgcagctc ccatagtatt
     4021 gacattcttt atagtttctt tcgggttgat aataactttg ataattgtaa gattgattga
     4081 tgaaatgaaa tcagtagcca ctgtattacc caaaattgtc cctgatatat atgcctactt
     4141 aaatatgctt ctaaataagg ggatagatat atttgaatgg cttcctcctg aaattactga
     4201 taatattgga aatttagttg caaaactttc ggaatcactt tcaaatatac ttaactcaat
     4261 aataaaaggt gcttatatta ctgcagtatc aatacctgaa gctttagtat tcacagttat
     4321 aacaattgtt tcaacatatt ttatgtcaag cgacagagag cgcatatcaa agtttttttc
     4381 ctcccagttt cctgaaaaat ggattaacaa aggcaaaagc ataatagaaa atatgttttc
     4441 cgctctcatc ggatatataa aagctcagct tatattgatg atgataactt ttacggaact
     4501 tttcattggc tttactctaa taagagtaag atattcactg ctgctggcat ttataataag
     4561 ttttttggat gctctgccca tattgggaac aggaggtttt ctaacgccct gggccattta
     4621 ttcattcctt acgggaaata tcagacttgg aatatcattg cttattttat atggtgttgt
     4681 tcttatagtg cggcaatcca ttgaacctag aattctgggg caacagatag gagtgtatcc
     4741 tctggttact ttaattgcca tgtattctgg actgaaattg tttggtttta ttggatttat
     4801 acttggacct atactggtat tgatgttcaa gaatattttg tcaggaatac tcaagaacag
     4861 atccattaag gatatactgg aacaccaatc atgatatatt acccttgcat caaaaatatt
     4921 tacaatgtac aagcacaaag cgtttttgtt tttcatatat ttataaggta ctgcttatac
     4981 ggaggtgcct tatttgttta ccggaatatt ttctgcaata ttcgagacac ttagcaatgc
     5041 atgtttttta ttggggtatg tatctaatcc taactccttt ccacagccac taacacctga
     5101 ggaggaagcc aggtttctgg aagagtataa aaacggaagt gacgacgcaa ggaatatatt
     5161 aatagaaaga aatttaaggt tagtagctca tattgtaaag aaatacagca ataactgtga
     5221 agacaccgat gacctaatat caatagggac aatagggctt ataaaagcta tttccacttt
     5281 tgataatgaa aagggagcac ggcttgcaac ttatgctgca agatgcattg aaaatgagat
     5341 ccttatgcag ctcagatata caaagaaaat ccaaaatgaa gtatatctgc aggacccaat
     5401 aggcgtagat cgggatggca atgaaatatc tttaattgat attattgaaa atgacaatga
     5461 atctatagta gataaagtcg agttgaaaat gcaggtaaag aaactatacg atataatgaa
     5521 atctgctctt aagaacagag aaaaaattgt cctggaactg agatatggtc tttgtaacgg
     5581 taccagcaaa acccagagag aaatcgcaaa aatgcttggg atatcaagat cttatgtatc
     5641 aaggattgag aaaaaagcca ttaaaaagtt aaccagggag ttaaaacccg aaaattgtaa
     5701 ttaaattata attagcttat aataaaactg taagcaactg gcattttata tttgcctgga
     5761 tttagaattc cgggcaatta actttttaaa atcttttttc cataattttt ttagcaattt
     5821 cctcaaacac cggaccggca gcggatcctc ccatccttcc attttcaaca aatactgcta
     5881 tggaatacct ggggttcttt ctgggaaagt atcctgcaaa ccaagcatgg accacccgct
     5941 gaccctctat atattgaccc gtctcggcac ttcctgtttt tcctgcagct cctccgtatt
     6001 cgttaatgtc taccatagtt cctgtgcctt catctatcac cttttccatt aaattcttta
     6061 ttctatctgc aatatccttt tctattaccc tgtgcccctg cttgcgcttt acttcccgga
     6121 ctttgtttcc ataatcatca acaattgagt caactatatt tacactgttc tttatgcccc
     6181 cgtttgccac agttgcaaca atatctgcta cctgcaacgg tgtagccata attgcgccct
     6241 gccctatcga tatatttgca atatctccat cggaatacca gccatcaagt gaaggaatat
     6301 ttccaccgga ttcatcaatc ccctgttccc ttattcccgt tatatttccc aacccgaatc
     6361 ttcgtgcttt ttccagtaaa ctctttttat ccatcttaat agctgcatca ataaaataag
     6421 tattgcagga taaagcaaaa gctttatcaa agtcaacata tccatgtccg cctttatcat
     6481 aggaatagca tttaaactct ctgtctccta cctttattga acccgtgcaa aaatactctt
     6541 catcccaatg ttcctttaac tcaaacatag cagctgcatc gattattttg aatatagaac
     6601 ctaaattata tgaagctaca gctctattaa acaattcctt cccggtactg ttaagataat
     6661 ttcctatatt gtactgatca aaatccggtt tgcttgctat ggcggtaata ttaccgctat
     6721 ccacttcttc taccactaca gctcctgtta tattgtgttt atccataaca tcttctacaa
     6781 ttttctgtat atggtaatcc aaagttaatt ttacattaag ttttttattc ctgccctgta
     6841 ctttcattaa ccgatatccc atgccgggta atacattgtt ttttccatcg gttattaccc
     6901 caacaaagct ttccgtatcg tattcaagag catcattata aaacttttca agacctgctt
     6961 ctcctgcgcc atcaatttta ttaacatatc ccagaatatg ccgtgcaaga gtagtatcgt
     7021 catacctttt taaggaatta ataatagaaa tcccctcatg ctttaaatca ttaattatat
     7081 tttttttctt ttcatctgcc tctactacat aaggctttct actaaaagct atatctctca
     7141 taacattttc aaaatctatt tcgagaatat tacaaatatt tttgagatca ttttcatgac
     7201 cctgtaacag caaaggtttc aatagtacaa gatacttttg tattctattt gtaaaaggga
     7261 tttcatttct atccagtata tcccctctga gtttttcaat ttcaaggctt gatatccttt
     7321 gtgcagaagc aagccttgat aatttttctc cttgaactat ttgtaaatta aaaaatctat
     7381 atccaagtcc aataaatatt gcactaaaga ttaaaaggac agcaattaat cttttcgcct
     7441 ccattttaac ggttcatctc ctttttatat gttttattaa attctcccgt aaaatttcat
     7501 attttataca agtaaacgta atttttctgt gttttcaggt ctttttttct attctctatt
     7561 tatatcaatt gttttataaa attgccttta caaaatcgca cacatgttct ataatgtact
     7621 tatgaacgtg cgttccgttt atttttggaa ttaaggtgat gacatgtgga tagaaccatt
     7681 cttcattgtg atttgaataa tttttacgca agcgtagaat gcctttataa tccagacatt
     7741 agagataagc ctgtagccgt atgcggctct caatcaacca ggcatggtat agtacttgcc
     7801 aaaaactaca tagcaaaaaa gtatggggtt ataaccggag aacccgcctg ggaagcaaag
     7861 agaaagtgcc cggggcttgt tactgttagt cccaattatc tcttatatct caggttttca
     7921 actaatgcaa ttaatattta cagaagatat acagatttaa tagaaccttt cggcatagat
     7981 gagtgttggc ttgatgtaac ggacagcatc cgcctgttcg gcagcggtag tgatattgca
     8041 tataaaatta agggtcaaat aaaagaagaa ttgggtgtta ctgcatcggt aggagtgagt
     8101 tacaataaaa tatttgcaaa acttggttca gatttgaaaa agcccgatgc tgttactgaa
     8161 attaccagta gaaattttaa agatacttta tggcctctcc ctgtaagaga attaatgtat
     8221 gttggaaagt caacggaagc catgttgaaa aaagcaggca tatataccat aggaaacctt
     8281 gcctgcaccc ctcctgcttt ccttatacgt cacctcggaa aatggggtga aagcttatgg
     8341 gtgtttgcca acggctatga tgatttgtcc gtatcaaaag ctgatacggg gcctttaata
     8401 aagggaatag ggaattcctt gacaaccccc agggacttaa cctgcaatga agatgcaaaa
     8461 atactgtttt atgtgctttc tgaaagtgta gccctgcgtc tcaggaaaaa caaccttaaa
     8521 gggaaaaccg tccagataac aataagagat tccaatatgg tctctattga gcgccagggt
     8581 cagcttaaat tacatacatt tatatcgtcg gaaatagctg aaaaggccat ggaaatattt
     8641 gcatattcct ggaattggtc aaataatata agattgttgg gcgtaagagt aacagacctt
     8701 gtttcagcag attcctatgt acagctttct ttttttaatg atgataagag agctaaaaag
     8761 cagcttttag agcaaagcat tgataaaata aggtccaggt ttggctacta ctcagtacaa
     8821 agagcacttt tgattaaaga cagcaattta aacgcaaatc cggctgaaga taatgttatc
     8881 catccggtgt catatataag gtaatgtatg gtgatgtata atgaagaagg tttttgttga
     8941 agttatagta aagtatttgt gtgatggtac gaagatacct ctttcaatta catgggaaaa
     9001 tggtaaggaa tatataattg ataaagtcac ggatataagg cgggctgcct ctctaaaagc
     9061 cggaggccag ggagtgcggt acaagtgccg aattttagga aaagaaagat atttatggtt
     9121 tgaagatgat ggacgatggt ttgtagaagg gaaataaggc attggatata ctaagccccc
     9181 ggaacttaag cgggctttat atattggggt tttcgcctga gaatagcgta ttctgcaact
     9241 tccctggcca tgggcatgta aaccgtcatc tggggatgag gtgcggaatc tatattttct
     9301 ccatcttcat ttttcatact ctcaattacc tgagtgaagt attcaccacc gggtatcact
     9361 acttctattt cttcaccgtt aaacatacgg tttctctgtt caactgtggc aatacctgtt
     9421 gccttatcat aattcctgac cagacctacg aaatcataat ctctaatata tgaacttgta
     9481 ttatatacct gctcttccgc cccgggtttc ttaaaataaa aacctgttgt atattccctg
     9541 tggcttgcct tggatatctc ctccatccac atgggatcaa atctgtagct ttccggatca
     9601 ttgcagtagg aatctataac cttgcgatat gctcttacta ctgtagcaac atagtaggaa
     9661 cttttcatgc gcccttcaat tttcacactt gtaactccag cctgtataat ttcaggtata
     9721 tgttctatca tgcacaggtc ttttgaattg aaaataaaag taccgctttc attttcaaaa
     9781 acaggcatat attctcccgg tcttttttct tccattagaa agtacttcca tctgcatggg
     9841 tgggcgcata aacctttatt ggaatcccgc cccgccatat aattactgag aaggcatctt
     9901 cctgagtagg atatgcacat tgccccatgt ataaatatct ccaattccag ttttttgtta
     9961 actttatgtc ttatttcacc aatttctttc aaagaaagtt ctcttgctaa tattatacgc
    10021 tttataccat gctcatacca aaattgtgcg cttgcccaat ttgtattatt tgcctgcgta
    10081 cttaagtgta tctccatttc cggagctgct tctttaacca gcataaatat tccgggatct
    10141 gataatatta aagcatctgc acctattctc tgcatttgag ctgcataatc ctgtatacca
    10201 attaaatccg aattatgggg tattatattc atagtcacat atactctctt gcccctggaa
    10261 tgggcatact caattccttc tttcatttct tcaagagaaa aattgtctgc atatgccctt
    10321 aggccatatg cctctccacc taaatataca gcgtccgcac cgtatataat tgccattttc
    10381 aatttttcca ggtttcctgc aggagcaagc agttcgattt tatccaatat tatcacctct
    10441 tttaaaactt acagcaactc cgtctcctac aggcaatatg catgtttcaa gactattatc
    10501 gctgcagatg gtatctaggt aatctttcaa tcttttcaca atagtttttt tccttcttac
    10561 taccagttct tcatttgcaa ccatgccttt atagagtaca ttgtcggata tcaatacacc
    10621 gttattattg agcatgcgaa tacaatgggg aagaaattca gggtattgtc ccttggccgc
    10681 gtcaagaaaa ataaagtcat atttcttatc aaggcagggt aaaacctcag ctgcatctcc
    10741 cgcaataata tttatgacgt ccttacatcc tgctatcttt atattttctt ttgcaagatc
    10801 tatcatggca tcattacttt ctatggtatc aataattcct ccttggttta aaatctgtga
    10861 cattaaaatg gaagaatatc ctatagcagt acctatttca agtactttgg acggttttac
    10921 catatggccc aaaacaacca atagccttgc aacctcagga tgaacaattg ggacattatt
    10981 taataatgca taattgcgca gttccagtaa aatgccttca tcttttttta ttgtatctct
    11041 tatatattta tttatataat cataacagat cataatacca aatgaccttc ttttttattt
    11101 aattaccgta tatttcggca aaatacaggt ggtgccaggc ataccgggtg cattcaatct
    11161 atttcttcac ttttaataaa tgcacccaga tacacacatt tttacattta tttagcattc
    11221 aactgagcct taagctgtgc tcccaggtgc tcagtgtaag tctttgtaaa ataatgctca
    11281 cctgtaccat ctctctttaa ggcgaaatac aaataatcat gttctttagg ataaagggct
    11341 gcttcaatag attcctgtcc cgggttgcaa ataggccctg ggggcagtcc ctcatataaa
    11401 taagtattat atgggtcttc tattttctca tcctctatac ttattatttc tttcatttta
    11461 ccttctcttt tgtatagaac atattgaact gtggcgcaag actgaagttt ccttaaagaa
    11521 ccgtttttgc ttcgcaacct gttgtaaaat actcctgcaa ttataggtct ttcttcagct
    11581 acttttgctt ctctttcaat aattgaagct agaataatta tatcatccac cgacatattt
    11641 aattcttcag ctcgtatata atactcaggt ttaaattttg tatcaaagtt tttaagcatt
    11701 ttatgaatta tttctttttc tcctgacttg ggatcaaatt cataggtatc aggaaacaaa
    11761 tatccttcaa gcctgttttc tctttcaggc aaatttttca aaaacttaaa atcaaatttt
    11821 acgcttttct ccgctgcaat aaagttcttt ttatttgcaa ggtttgcttt ttcaagcagc
    11881 tctattattt gactattatt atacccctca ggtatcataa ctctaactcc aacaaagttc
    11941 aatggactgc ctgacataat ttccataagg atgtcatacc cttttaatga attatagttt
    12001 gtatttttat caattgcatg aactcctgac ctgtaagtac catcgtaacc gtttactcta
    12061 gacagcattc taaaaatgaa agcgttttta attacacctt ctttatttag aatatttgct
    12121 atagcttctg ttcctgaacc cgtgggtatt tcaattaata attgatcttc cagcaatatc
    12181 tgctgtattg tacttactgc actgtagttt tttacaatat atgaatatgt cgcatatcca
    12241 catccaaata ctatagccag cattaagaca gctatagacc acctgataat tgattttttc
    12301 ataaaaccac catcaccctt tacttacagg tcttgttctt cacctattaa ttttatccct
    12361 tgcttctggc ttttgccctc tgttctgtat caagtatctt ttttcttaat cttatatttt
    12421 taggagtaat ttcaaccaac tcatcatcgg agataaattc aagagcctgt tcaagagata
    12481 aaacctttgg tgtagtcagt ctcaaggctt catcggaacc tgaggcccgc atattggtta
    12541 catgtttttt cttgcataca ttaactacaa tatcttcagg acgtgagttt tctccgacca
    12601 ccattccttc atatactttt actccaggac ctataaacag ggttcccctt tcctgggcat
    12661 tatataaccc atatgttata gattcccctt cttcccaggc aattatagag cctcttggcc
    12721 taacctgcaa atctccttta tatggttcat atccataaaa gacatgattc ataataccat
    12781 ttccctttgt gtctgttaaa aactctgaac ggtatcctat caatcccctt gcaggtattt
    12841 taaattcaag ccgcatatag ccttcattgg ctgaatgcat attcaccatt tcagcctttc
    12901 ttggtccaat cttttccata accataccca tgtaatcttc ctgcacgtca attaatagat
    12961 attctatagg ttcatgtaat actccttcta tttccttcat tattactctt ggttttgaaa
    13021 cctgaaactc atatccctgt cttctcatgg tttctatcag tattgaaaga tgcagttcgc
    13081 ctctccctga aatattaaat gcatcaggtg tatcagtttc ttcaaccctc aaactaacgt
    13141 cattttccag ttcacggaac agcctgtctc ttaaatgcct tgaagttaca taagtgcctt
    13201 cccgtccagc gaatggactg ttgttaacca gaaaagtcat agataccgta ggctcgtcaa
    13261 tattaataaa aggaagaggc tcaggattat ccaaatcaca tattgtttct ccaatattga
    13321 tgccttccaa tccgggtact gccactatat cacctacagc agcttctgaa cattcaattt
    13381 tctttaaccc ttcaaaaata tataccctgc taagcttact ttcctgttta gtaccatcct
    13441 ttttacatat aacagcctgc tgaccccatt tgatttttcc cctttcaata cgtcctatgg
    13501 ctacacgtcc ggtatattcg ttataatcga tgctggacac cagcatttgt aatgaacctt
    13561 cagtatcacc ttttggagca ggaaccttat caagtattac cttaaacaaa ggttcaagat
    13621 ctactctttc atcgttcagg tcaagtatag ctataccatc tcttgcagct gcatatacta
    13681 tagggaaatc aatctgccca tcatcagctc ccagctcaat aaataattcc aaaacctgat
    13741 ctactacttc aagaggcctt gcaccaggtc tgtctatttt attgataact acaatcggct
    13801 ttaaattcat ctgtaacgcc tttttcaaaa cgaatctggt ttgaggcatt ggtccatcaa
    13861 atgcatcaac cagaagaaga acaccatcaa ccattttaag cgttctttca acttctcctc
    13921 caaaatcagc atgacccggt gtgtcgatta tgtttattct aactccttta tataccaccg
    13981 cagtattttt agccaatata gttattccac gttctctttc caagtcgttt gaatccatta
    14041 ctcgttcctg taaacgttca ttttccctaa atattccgct ctgcctaagc atcccgtcaa
    14101 ccaatgttgt ttttccatgg tccacatgag caattattgc tacatttctt atgtcgtctc
    14161 taacttttat cacaatatta tccctcttct ttttaagttt agcacttgtg tagttttaac
    14221 ttagtatttg tacagttttg tatgttaata atttattgcc tgtattgtct gcaaaatatt
    14281 cttagcgtaa ttttaaaaac aaatcttatt ttaaattatt ggtgtcaggt tgtcaacttt
    14341 ttagttcaac aacagtgacc ccggtttctc cctcaccaaa tttaccaagc ctgaaggatt
    14401 gtacatgagg atgggtcctc agatgcctgt gaatacctgc tcgcagtata cctgtcccct
    14461 tgccgtgaat caaggttact gatttcagtc ctgtcatgct ggcatcatcc aggtatttat
    14521 cgacactatc aatagcatca tcaaggttaa accctcttaa atcaatctct gttgatatat
    14581 tcatggcttt tgctctgcct atttcccctg caccaacttt ttctatttca attttttgtt
    14641 catctacaag ttttaaattg gtaacatgaa catttatctt cattatccct gcctggatta
    14701 tggcttcacc attagctccg ggaggctcta ttactatacc cttctgaccc aggttgacaa
    14761 taagtaccgt atcccccggc ttaagattct caggaggctt tacataactc tgtctcggca
    14821 ttatggtttc cacaagagac tcatctattt tattgacttt attcctgagt ttttgtttga
    14881 tttcttctaa agctctgctt ttatggacat tttgccggtc cttctcaatt ctttttactt
    14941 cttcaataac agcttcagct tctgcctttg cccccgttat aattcgtttg gactcttccc
    15001 ttgcttctct gatgatttta tccttttcag agctgatctt ccttttctgt tcttccagtt
    15061 catttttcag tttctcaatc tcaagcctta aagcttcagc ttttaccctt tctgtttcag
    15121 cctttgtacg gtccttctcc atagacaaga gtacatcctc aaaccttata tcttcctggg
    15181 taagataatc cctggccctg tcaagcacct cttcctgaag cccaagtctt tttgagattg
    15241 caaaagcatt actcttgccc ggtacaccta tcagcagtct gtaggtaggt ctcagagtct
    15301 ctacatcaaa ctcgcagctg gcattttcaa catgttcgga agtcatagcg taaattttta
    15361 attcactgta atgtgttgtt gcaacggtaa ttgcccgctt ttcatgcagc tcctcaagta
    15421 ttgccattgc aagggctgca ccttctgtag gatcagtccc ggctcccagt tcatctaaaa
    15481 gaacaagaga ttgactatct gcattttcca gtattttgac tatattggtc atatgtgagg
    15541 aaaacgtact caaactctgc tcaatactct gttcatcacc tatatctgca aaaattttat
    15601 taaatacaca aatctccgtg ttatctcctg caggtatatt tagtcccgcc tgggtcataa
    15661 gagcaaataa ccctacagtt tttaaagtta ctgtttttcc tcccgtatta ggtcctgtta
    15721 caataagtga cgtgaacctt tctcccaccc aaaaatcaat aggaacaaca gtttcagcat
    15781 tcaacagggg gtgtcttcct tttcttatca taatacagcc atcagtattt aactttggag
    15841 aaacgccgtt atactttaga cttaaattag cttttgcaag tataaaatca agctttgcaa
    15901 gtatggacat atctgtcttt aattgaggca atataccagc tacatcctct gtaagttcag
    15961 ccagtatccg ttcaatctca gcttgctctt tgatctgcag ctgttttata ctgttatttg
    16021 cttctacaac agccataggc tctatgaaca tagttgcacc gcttgccgat gaatcatgaa
    16081 ccagtcccgg aacctcatta cgatattcct gctttacagg aactacatat cgtcctgctc
    16141 ttatagtaac gataggatcc tgcatatatt tctggtaatg gggtgatttt attattccat
    16201 tcaacttttc cttaattgat tcctgacgtt ctcttatttg tcttcttata ctgttcaaag
    16261 tagggcttgc atcgtctgcc atttcttctt cacttacaat tgaataactt atcttatcct
    16321 caattctctt atttgcttca aggcagtcaa taaacccaaa aattacatta ttctcttcat
    16381 cgacttttct atcacctgca gcatggcttt tcaagtttct gcacagtctc agtacatcag
    16441 caactcttaa tagatctccc atgtttagaa cagcccccat atccaccctt ttcaaactgt
    16501 cccttatatc acggatccca cccaaagaag gattgcctct cctcaatata aagctgaccc
    16561 cgtcatcagt ttccctcagc ctgtctgcaa caacagtcat gtcattggaa ggcataaggc
    16621 tctcagctat ttctctgccc agctgggaag ctgtaaaacc ggcaagttga tttataattt
    16681 tatcaaattc taaaactttc aaggtttttt cattcattta tctgtccctc tataataaac
    16741 taataataaa taactatttg aataatccgt atattgttgc tttgaatgcc agattatatc
    16801 tattatattt taccagagct atttaaaaga tacaaatgaa tatttaaaaa aaataatgcc
    16861 tgcttttgca ggcaaatgta gtttcttttg tacgttatct atatttaagc tcatctataa
    16921 gcaagcctga ggcttttctc ataccaagtc ctgatttatc aagtactttc atggtatttt
    16981 catactcaat atgtctttta atttttaagg tagttgtttt aataagatct tcataggtca
    17041 taacaaaata ccggataatt ttatactgag gtacattttt atttatttct tttatagcat
    17101 tattaaaata tgctgccagt tttgtttcaa gcgttgtatc atctgcgaat ttcgaatgtg
    17161 tatcttctgt taaggcagga aggttctcct tatcaactac aatttttgca catacctgaa
    17221 tacccccatc aaaagccctg ttgccccaaa caaaagaatc ctttactccc ggaatattgt
    17281 tgagcaatac ctcgtattcc tccgggaatg cttttttacc atttgtaaat acaatcatgg
    17341 tcttggctcg gcccgttatt tttaaaaaac cattgtcatc aacaattccc agatcaccgg
    17401 ttctgaacca accatcttta tacatgacct gtgccgtagt atcaggatct tcataataac
    17461 ccagcattac attgccgccc cgggtgataa tttctcccat tccattttca tcaggagaat
    17521 ctatggctac ctcaattcca cccagagggt gccctacagt gccaggttta ctggcaaaat
    17581 cattgtttgc acatataaca ggagatgtct ctgtgagacc ataaccttga agcagataaa
    17641 gccctatttt ttcaaatccg ataaccacat ccggatctaa gggagcagca cctgaaactg
    17701 ccagtcttaa gcccggccct atctgttcaa atacacttct aaa
//
LOCUS       DAOSDD010000074        17711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1297_length_17711_cov_6.086713, whole genome shotgun sequence.
ACCESSION   DAOSDD010000074 DAOSDD010000000
VERSION     DAOSDD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1297_length_17711_cov_6.086713"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..5612)
                     /locus_tag="PK733_08365"
     CDS             complement(63..5612)
                     /locus_tag="PK733_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10592.1"
                     /translation="MKNTRKISLILIISIILSLFVIPNISLLTVDAASDSEKYYEQGY
                     YPIFYSESNKATFDSLKDILPAGTPSTITKDGNVYPFNYRLWFAKNVVVYGDYSTVNS
                     RPNDFKAETDSPHGTGYYSKSGVRGEYRYHGYDRVGNKYNNTWWTLDVVTGSSINDNY
                     WLYRPWDYPNKIEKNISLYNAAAERYKTPEDINTRDWINRPIEKDGDLLTFANAEISS
                     EREAYRYIHIMSPPTINYPGEGKAWFKYNNDLLYRTFTIDKLDHVDKLMTDVVTSVKI
                     LTSENRFTILDFGPENTPEYLDREIEVEVEVAGVLLDEEYYRDPVKKTARYTREDIKD
                     WTISLGSHIQNNVKSADYNKGKAKFILRYKIASILAQNTYTLNGNTRVNFKNNKSNEN
                     NGSANLAFKIQKVPPSNKITKGKLMTEVETTIEVLTPPEYLNILDYGEINGPEYLDSF
                     VNVQVKVKGVLQDDTFYNDLKEREKRYNRDDIIRWDISLGGLTESVSPNTYQKNAGEA
                     VFTLQFKRRDIFDHQSWPMNGYVKVYFNDSKDNKDNSDTTIVFTITKVPAGETPKFDD
                     NEELPPPPPPPPPPIIVQPLCHIPNIGFDIVPFPAYDETDPGTVLLREVYINGEIVDD
                     DLFFSGNYVFGDEKDGMKRIEVYYESVEGIISTSIHWAYIYDTKPVAQFKLDGTFKEN
                     RKLSAVENCTIGNTQIVLDHYPIISYEWKFITVDGESSSLKQRTPTSDKHREFLYKKP
                     GVYRLELTITNTLGRVSKPYTLDFVICEDTPPAIEMNIWNGVLTRGEELKLHYSASSV
                     DNDTVASKTLQLYYDSNNDGVCDQLVKTFPNGQFTGYAPNKLGKYKLVAIATEEFGQD
                     TLTEFITAADKKKKTIESEFWVDNLIPMTDIYVDIPIVRPEIDLYIMMDKSLSDTKKQ
                     YITENRINFNNILRTKNILPKVETWDMKTYEYSQTVSDTYRSGESDPPSSISYSSGGY
                     SGTLYRDRVVDNGEYIDYGGWETETEYKTFTDSDSYTITKVYRDGSLESTEGSEAPSS
                     IYINQDGYSGYIYGTTSDPSISSSTSYDSESRYFTASHSNTVETWYHISGKSQTYESD
                     PAPSSYNIDEDGYSGYIQRTGTSGPYDYQHYSSDEWDVYIQTWVAEYSGYLYKSVPVE
                     TVYESYTVSYSGTLSKEVDVYVPDIRWRSDYIGYYSGTIYKNVRQDYINPFRATSDKY
                     VVYVSDGNINELTDLGMVMSKADVRLILVGSSSIRTQIAHDHYISKDKPIEEIMQEVL
                     DYIATNSPAVEKHYVLIGVDTFDIKTMDVDEENDPIVEKKFQYVQNPSYFDNPTGMES
                     FAAASFSENSNWVDTKVSNFNRVGEFRIFRRIKDQPSPDPNFANYSYYSGTPEIRIYA
                     HRKPIVRAILDWDYDNTKGVYKTKWVCQSYDLDHQYSREDKGIVERKIMFRRTGGEWY
                     YYIPEELSPGTYELQYYVKDVEGVWSDPFTMNFTLASAPSIQFDAKLRPKDSSFSLSN
                     IPASEYLEVYDVWTRYPYDIKLEMVLKDGTISRKQTQYYNSTRDTKTGNDIHWGSINY
                     EIPAAVKDGTYTFEIAAIDITNPSKKEVKVFTVIVNTPINLAPSMTETVMTETTVNVK
                     ATTTRYANTTRAILFYGTAYQKTLDLIGIQEGNMKNWANRYTIPTNIPEGTYTARFTA
                     TTLSGKSETKNVQFRVESLMITDITIEGGWNHWRGQADLFDPQIILTNEPHRFLSLEE
                     VTVRVSTAGYAEKVEIRFSPELEAMQFIDAYGNIYDYKNDFGLDYISFPKVILLDSTK
                     KEYTEEWKYILPLANDTKAWDNVRKNQPYKMTVTAWKGTTSVTEEISDIEITGSIYDL
                     IYMQPIN"
     gene            complement(5664..6920)
                     /locus_tag="PK733_08370"
     CDS             complement(5664..6920)
                     /locus_tag="PK733_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10593.1"
                     /translation="MNKKIKLAVILCLITTLLFANIVPVYADSEGTTIINKITTTLSD
                     LINPLLEKLASFKIESVTNAVNKFTDMLTHWSREYVGKLNLLEIIAGYGDGRFGPEDT
                     LKVDEFLKMTLRAMGHKVEEGIDYWAEPYIKLAKEEKIIDEKEFTDYRRPILRQEAAR
                     IIVKAALKTEEAPIPNHTSYAKLRIPDYYDIGDDYKQHVLYSYSMGFITGISDGSFLP
                     KKTLTRGEGSAIVMRYLDKGLRKPMKPKDEEIAVITDTYTGVTYEIYPPSKPEVIDVI
                     KVMRDSMGKSKGFPDIGYNATDELVFLSFYKSKEAYEESSIFIDSGIMIYMPEWYLNG
                     YSITIHEPEAVKELHRDVYIELFNHLFEKEADKVIKDFDYCIELGIKGGELKKQYKAN
                     NRSVYFTKFEGDKSTGLYVGFKETNN"
     gene            complement(6977..7618)
                     /locus_tag="PK733_08375"
     CDS             complement(6977..7618)
                     /locus_tag="PK733_08375"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOJ10594.1"
                     /translation="MKRTKLIIITVILSVVLLAVQTYIVKTGSEYEAKVKVVFAKEYI
                     PANTEITEDMLEMREVNIGLAHLKSVTNAGKLIGKKTKMDIEAKEMILESKIYKENEL
                     MEKIAVKNDNNRLFTLELKGDQANGWWIEADQYVDILFVPTQKPEERKVERMEKIRVA
                     ALISQEGKLIKNNIDRNVIPKYICLEVDTKQDELLAFAKGNGRIELSVIPSRE"
     gene            complement(7659..8207)
                     /locus_tag="PK733_08380"
     CDS             complement(7659..8207)
                     /locus_tag="PK733_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10595.1"
                     /translation="MIEVKDMIKNNKGSATAHVIALLGLIIFIILPLFAVVMERYILY
                     NKVNIIKDAVDVANLASYNAISTEDAAKNDAAIRLNSEITIYKNILAKNLKLKADMTP
                     EGNSIAEGEVVIDELKAYLHINTTFPITCSQGNSITRPTMHSVIKVPIKPMLFRRVIL
                     SAIGKDVIYIKVHVDTELPINN"
     gene            complement(8204..8584)
                     /locus_tag="PK733_08385"
     CDS             complement(8204..8584)
                     /locus_tag="PK733_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10596.1"
                     /translation="MDNIIIFSIITVVIISLIVGIVWMFVPMSKKNAMDIECRKTLLK
                     MENDNGLTITEEINLQNKLTSLGFKEITITGTPTFTKKKGEELNLKVEVKYDYRSFKS
                     MFTTEKTTSTMVYDRTITSRQVVK"
     gene            complement(8666..8926)
                     /locus_tag="PK733_08390"
     CDS             complement(8666..8926)
                     /locus_tag="PK733_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10597.1"
                     /translation="MKNIKEKLIVTELKMRSNLMTSKEKAKTALKDNKGSAVSYILIL
                     VAGVIITVVVMYPALDTLYESIAGHLQDWWSRVTTKVFIDTI"
     gene            complement(8967..9875)
                     /locus_tag="PK733_08395"
     CDS             complement(8967..9875)
                     /locus_tag="PK733_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10598.1"
                     /translation="MNEIINLAKSNIVFSIIMLILNGIIISNLLLKLKLKRGRYSREA
                     KGSRTSEKALFLKIRRKATLELEKVSGGDKERDIYTKAKEKVKKAGYKNKHAALIYLA
                     CKYIVPLLIFLIAMLTNYRKNILGPIIAATACYVTTELVIYQNKIEINKKFQRHVYKI
                     YKFLHNQISSGVTIPEAIKIVYQIMDDEGIKMSLIRMAATYELTTDIDLAIQEISNEY
                     DNLEADTLVIAIKQGVATGGEGNLLEQQEELMFNKYMNYIQAETDRRTYIGIAAALSF
                     AFIIVLMVTMPMIMEALSGIEEMFSF"
     gene            complement(9966..10931)
                     /locus_tag="PK733_08400"
     CDS             complement(9966..10931)
                     /locus_tag="PK733_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ10599.1"
                     /translation="MREIIFYIVSALLVISTVVLSFLIAEQIVYKKIIHIKLTNMNKK
                     LEERLIENEIKKYTTKLDTKKGFIERIEINLIHRTNVNKKFRYIQINAYILLIFMLII
                     FLLSIKPAYRMVNWLPTSIIISAIISMIPVVILDMIGKINSQKTAQQLAGFITILNRW
                     CSVKDDIFYAFEKSVESLDYPLRTYIAEMVIQIKSGIDPEIALDILQIKVNNEQFSEF
                     VINIKQNIKSRGDTKKLLTNMESQFYKIKNEMDRRKISTLTDRVVIYIIMGLVFVVAT
                     AILKYSLEGRAYYLYTEQGRILLAIFSLVYAVGIYLTMKITQFRY"
     gene            complement(10928..12181)
                     /locus_tag="PK733_08405"
     CDS             complement(10928..12181)
                     /locus_tag="PK733_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ10600.1"
                     /translation="MPTVLTLNQLLEGSQVKTNRTAESNMNDIVTEITFNILQESPKL
                     VADIANGIIDKKVLETAIIKNIDKNKYNILERDKFIKRVFDFMFGYGPLQEYLEDESI
                     SDIDGTKYNEFTITRKGMRQKIDIDFGSEEQFDKYCKLLVRRNYGIINENDSHCRVTD
                     NQKRLRINVSVPPRNISGPAISIRKHPEKSLTLDELQKEGMLDEEIEVFLKKLAVSDA
                     NVVISGKGAAGKTTLLKAIINNMPELDRVLICEKDAEIFPEKPNCIEQRVKKEGEGGR
                     TVTLRDLIKDGLTMSLDTYVIGESVEAETWEGIRAALSGHRFLTTTHSRKAYDCLERL
                     LTLSKMANIGESEDTVKDIIGRSIDCIIHMEKFKIVNIVEILGYDKAKDDFIYNELYK
                     YDKTKHLKTNNFRERLNDKLGGEEI"
     gene            complement(12203..12994)
                     /locus_tag="PK733_08410"
     CDS             complement(12203..12994)
                     /locus_tag="PK733_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10601.1"
                     /translation="MNKIITIWGNPEFGCELAYRIAKETGKNILLLDVDLLSPKADLY
                     LNIKKESDDLLERKTGLDILIEWSNRKYLNGELIRKAAIQREELKNLYIITGNYDLDN
                     FEYYTIENLGDIIEKANQNFEITILLANKHIYDAYTITSLLRSDINIAAIEPNTVELR
                     EYNNYLMFLEDKQGMRLDKTKFVGYEYIAGIHETNAELKEITEDTYIGKVRYSKARVL
                     SRSKNKCYAKTMEKAVKRDYIKIIEQLELTPKLKTKENFKFKKID"
     gene            complement(13193..13948)
                     /locus_tag="PK733_08415"
     CDS             complement(13193..13948)
                     /locus_tag="PK733_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10602.1"
                     /translation="MAKLVGRYRRLARSKLKDSYTYDIGERYSVELGNDLNRVLSSEF
                     AMLSNPHSKLVFLKKYAGKQLKQFKKREKVGKGSGNMVICCDESGSMNSGKDFWSKAI
                     CCSLLEVALSNNKNFASIHFSSKGKCVTNEINKENYSLEAVMKMACNFLDGGTDFETP
                     LLEAKKLLETDKYKYADLVFITDGECDISDDFLKEFLKTKNEMKFNVIGVVLDKGIGS
                     VSDFTLKKFCDIIYKSSEFDEDDIAEKILSKVS"
     gene            complement(14339..15091)
                     /locus_tag="PK733_08420"
     CDS             complement(14339..15091)
                     /locus_tag="PK733_08420"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOJ10603.1"
                     /translation="MSRRKLEINKKHCSYEELEASYKDCNDAKLRTRMLAVLQTWDGK
                     PSIEVAKDIHMADTNVRKWVHRYNEYGIAGLIDARHSNRTSYLSPEQRQAVKEALLKS
                     PRECGFNKSNWNIPLLKLWISKQWGINYKASSLYDLVHKLGFTLQRPKKQSRNAKKEL
                     QDQYKKELQDILENSDDDTVILYEDEAIITDEPTTILKWSLKGNQPIIPTDSSGSRER
                     IVMFGAVNPIDGKVHYSTSEAGNSDSFKDFLK"
     gene            complement(15195..16031)
                     /locus_tag="PK733_08425"
     CDS             complement(15195..16031)
                     /locus_tag="PK733_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10604.1"
                     /translation="MKVNPNFEELFRSVLSPGWLKAADNILKASSDIGNDNVIKHSMI
                     DKMVYEEKRKEVEQDNGVLKNIEHLGQEKLKTFPALTQDIFNAFYNVKPEIKDESELS
                     LPAQKFNKHIIIKTMQDDEYHVLKQLTEGCDTESIEATKEFIVNIFDNLDELLKDVSG
                     EKKTLDVMEKKVLQSKEKQGELQDLLDKAKEFSEKGELEQSEIMIQQAIATAKDLEKQ
                     KKQIEYLEKIVDQNSAKNKEQIEQKISAALKKAINKTEEIKDILDAWGDNDPKYMATE
                     TI"
     gene            complement(16151..17398)
                     /locus_tag="PK733_08430"
     CDS             complement(16151..17398)
                     /locus_tag="PK733_08430"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10605.1"
                     /translation="MQSKKFNFSFELVDKLKAVMADVATLAIEKEELIEYLALAMLAR
                     MNIFLLGLPGNNKSDTIRNLVNRIDGAKHFEAQLHEQFNAEELFGVLDITELAKGNKR
                     IDTSNMLPEADVVVLEETFKARSVLNSLLKAMNEREYTNYGMTYDIPAFTFIGASNEI
                     PDTRDPEERVFAALIDRFEIKVKVDYITGKSNREKALNVVMQRKREKLLNKNLMVNAT
                     IPKSELEKMQKELLFVEVPTPIKELLEKILINLRAKGIHVTDRRYLNCTTLLQAVAYL
                     NKRNAVAIEDLAYLKNYFWDMPSDIPMVQEIIEQNIKNPMKESIDATMAIARDLYNHN
                     VETINKGLEEREKMVLCKKFRKEIVRIYDIMLGYDKNIRSDSDRELYIKSIDELEELN
                     MKINSALGFSQLPLKETKMMMDR"
     gene            complement(17484..>17711)
                     /locus_tag="PK733_08435"
     CDS             complement(17484..>17711)
                     /locus_tag="PK733_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10606.1"
                     /translation="SKSEKIIGRWLEKIKELNDKKGKYELLFNKSLSKLNDEFENVEF
                     LATELQMRLRETSNFKTGTKPLDGQIGFVAA"
BASE COUNT         4615 a         3441 c         2554 g         7101 t
ORIGIN      
        1 ttggtaatgt attcttccct tggtttattt ttcttcgaat gaaaaaatca cacccagtaa
       61 tattaattga ttggctgcat atagattaag tcgtaaatac tccctgttat ttctatatcg
      121 ctaatttctt cggttactga tgtggtacct ttccatgctg ttaccgtcat tttatatggt
      181 tgatttttgc gtacattatc ccatgctttt gtatcattag ccaatggtaa tatgtatttc
      241 cattcttctg tatattcttt ttttgtacta tctagtagaa taacttttgg gaaactgata
      301 taatctaaac caaaatcatt tttatagtcg tatatgtttc catatgcgtc tataaactgc
      361 atggcttcca attctggact aaaccttatt tcaacctttt ctgcatatcc agctgtactt
      421 acccttactg ttacctcttc taaacttaaa aatctatgcg gttcatttgt taatattatt
      481 tgtggatcaa acagatccgc ttgtcctctc caatgattcc aaccaccttc tatggttata
      541 tctgtaatca ttaatgattc aaccctgaac tgtacatttt ttgtttcact tttaccacta
      601 agtgtagttg ccgtgaacct agctgtatat gttccttcag gtatattggt aggtatggtg
      661 tatctgtttg cccaattttt catattacct tcttgaattc caataaggtc taatgtcttt
      721 tgatacgctg ttccataaaa caaaattgct cttgtagtat ttgcatacct tgttgttgtt
      781 gctttcacat ttactgtagt ttctgtcata accgtttcag tcattgatgg ggctaagtta
      841 ataggtgtat ttactattac ggtaaacact tttacttcct ttttgctagg attggtaata
      901 tcaatagcag ctatttcaaa tgtatatgtc ccatccttta ctgcagctgg tatttcatag
      961 ttaatactgc cccaatgtat atcattgcca gtcttcgtat ctcttgtaga gttgtaatat
     1021 tgggtttgct ttctacttat tgttccatct tttaaaacca tttccagttt tatatcgtaa
     1081 gggtatcttg tccatacgtc atatacctcc aggtattcgg aagcaggaat attgctaagg
     1141 ctaaaacttg agtccttggg acgtagtttt gcatcaaatt gaatggatgg tgcggatgct
     1201 aatgtaaaat tcatagtaaa cggatctgac catactcctt caacatcttt aacatagtat
     1261 tgtaattcat aagttccagg tgataattct tccggaatat aataatacca ttcgccaccg
     1321 gtccttctga acattatttt tctttctaca attcccttat cctctcttga atattgatga
     1381 tcaaggtcat aggattggca cacccatttg gttttatata ctcctttggt attatcataa
     1441 tcccagtcta atatagctct tacaataggt tttctgtgag catatattct tatttcagga
     1501 gttcctgaat agtaggaata gtttgcaaag ttgggatcag gtgaaggttg gtcctttatt
     1561 cttctgaata tacggaactc tcctactcta ttaaaattac ttaccttggt atctacccaa
     1621 ttactatttt cagaaaagct tgctgctgca aaactttcca ttcctgttgg attatcgaag
     1681 tagcttggat tttgaacata ttggaatttt ttctccacaa ttggatcgtt ttcttcatca
     1741 acatccatag tttttatatc aaatgtatct actcctataa gtacataatg tttttctact
     1801 gctggactat ttgtggcaat atagtcaagt acctcctgca taatctcctc tataggctta
     1861 tcttttgata tataatggtc atgcgctatc tgtgtcctaa tagagctgct tcccacaagt
     1921 attagcctga catctgcctt tgacattacc attccaaggt ccgtaagctc atttatgttt
     1981 ccatcactta catacacaac atatttgtct gaagttgctc ggaatgggtt tatatagtcc
     2041 tgacgcacat tcttgtatat tgtaccggaa taatatccta tataatcact tctccatcta
     2101 atatcgggta catatacatc aacttcctta ctcaatgttc cgctgtagga gactgtataa
     2161 gattcataca cagtctctac cggaacactt ttataaagat accctgaata ttctgcaacc
     2221 catgtttgta tatatacgtc ccattcgtct gaagaataat gttgataatc atatggccca
     2281 ctggtacctg tcctttgaat ataaccgcta tagccatctt catcaatatt ataacttgaa
     2341 ggtgcagggt cgctctcata ggtttggctt tttccgctta tatggtacca tgtttctacc
     2401 gtattactgt gagatgcagt aaagtatctt gattcactat cataacttgt gctcgaactt
     2461 attgatgggt ctgatgttgt cccatatata taaccactat aaccatcctg attaatataa
     2521 atacttgaag gagcttcaga accttccgtg ctttctaatg atccatccct atatactttt
     2581 gttatggtat agctatccga atcggtaaaa gttttatatt ccgtttcagt ttcccaacct
     2641 ccataatcta tatattctcc attatctaca accctgtcac gataaagcgt tccagaatat
     2701 ccaccgcttg aatacgatat ggaagatgga gggtccgatt ctccggatct ataagtatca
     2761 cttaccgttt gcgaatattc ataggttttc atatcccagg tttcaacctt tggcagtata
     2821 ttctttgttc ttaaaatgtt gttaaagttt attctgtttt ctgttatata ctgtttttta
     2881 gtatcgctta aacttttatc catcatgata tataagtcta tttcaggcct tactatggga
     2941 atatctacat atatatctgt cattggtatt agattatcta cccaaaattc actctctatt
     3001 gttttcttct ttttatcggc agcagttata aattctgtta gagtatcctg cccgaattct
     3061 tcagttgcta ttgccaccag cttatatttc cccagcttgt tgggtgcata gcctgtaaat
     3121 tgaccattag gaaatgtttt caccagctgg tcgcatactc cgtcattatt gctgtcatag
     3181 tatagctgca gagtttttga tgctacggta tcattatcaa cactgcttgc cgaatagtgc
     3241 agttttaatt cctcacccct agttaatact ccgttccata tattcatttc tattgcaggc
     3301 ggagtatctt cgcatatgac aaagtctaag gtataaggct tggatactct gcctaatgta
     3361 tttgttatag ttaactccaa tctatatacc cccggcttct tatatagaaa ttccctgtgt
     3421 ttatctgatg ttggagttct ttgtttgagt gaacttgatt ccccatctac agtaataaat
     3481 ttccactcat aagatattat tgggtaatgg tccagcacta tttgagtatt gcctattgta
     3541 caattttcaa cggctgatag ctttctattt tccttaaatg tgccatccag tttaaattgt
     3601 gcaacaggtt ttgtgtcata tatatatgcc caatgaatag atgtgcttat tatgccttct
     3661 acactttcat aatagacttc tattcttttc atcccgtctt tttcgtcgcc gaatacatag
     3721 ttgccactaa aaaacagatc atcgtccact atttcaccgt ttatatatac ttctcttaat
     3781 aaaacagttc ctggatcggt ttcatcatag gcgggaaaag gtactatatc aaatcctata
     3841 ttgggtatat gacataaggg ttgaactata attggtggtg gtggaggcgg cgggggtggt
     3901 ggtaattcct cgttatcatc aaatttcggt gtttcccctg cgggcacttt tgtaattgtg
     3961 aatactatag ttgtatcgct attatctttg ttatctttac tgtcattaaa atataccttt
     4021 acatatccat tcattggcca tgattgatga tcaaatatat ctcttctctt aaattgtagc
     4081 gtaaatactg cttctcctgc attcttttga taggtatttg ggcttacgct ttccgttagc
     4141 ccacctaaag atatatccca tcttattata tcgtccctat tatatctttt ttccctttcc
     4201 ttcagatcat tataaaaagt atcatcctgg agtacacctt ttacctttac ctgaacattt
     4261 acaaagctgt ctaagtattc gggaccatta atttcaccat agtccaatat attgagatat
     4321 tccggtgggg tcaaaacctc aatcgtagtt tcaacctcag tcatgagttt cccttttgtg
     4381 attttgtttg atggtggtac cttttgaatt ttaaatgcta aattagcact gccattattc
     4441 tcattacttt tattattttt aaaatttacc ctcgtatttc cgttcaatgt atatgtattt
     4501 tgggcaagta tgcttgcaat tttatatctt aagataaatt ttgctttgcc tttattatag
     4561 tctgcagact ttacattgtt ctgtatatgg ctcccaagtg atattgtcca gtcttttata
     4621 tcctcccttg tgtaccttgc tgttttcttg acagggtctc tataatattc ttcatccagc
     4681 agtacgcctg ctacttctac ttctacctct atttctctat caaggtattc gggcgtattt
     4741 tcgggaccaa aatctagtat tgtaaaccgg ttttcagatg tcaatatctt tacggaagtt
     4801 actacatcgg tcataagctt gtctacatga tccaacttgt ctatggtgaa agtacggtac
     4861 aggagatcgt tgttatactt gaaccatgcc ttgccttcac cgggatagtt tatggtaggc
     4921 ggagacatta tatgtatata ccgataggcc tccctttcac ttgatatttc tgcgttagca
     4981 aaggttagaa gatcgccatc cttttcgatg ggccggttta tccagtctcg ggtgtttata
     5041 tcttcgggtg ttttatatct ttctgcagct gcgttataca gacttatgtt tttttctatc
     5101 ttgtttggat aatcccatgg tctgtataac caataattgt cgtttatgct tgaacctgta
     5161 accacatcta aggtccacca ggtattatta tacttgtttc ctactctatc gtaaccatga
     5221 taacggtact ctccccttac accactttta ctatagtagc ctgttccatg aggactgtcg
     5281 gtttcagcct taaagtcatt cggtcttgag ttgacggtag aataatctcc atacactaca
     5341 acgtttttag caaaccataa cctgtagtta aagggataaa cattaccatc cttggttatt
     5401 gtagaaggag ttcctgcggg taaaatatcc tttaaactat caaaagtagc tttattgctt
     5461 tcgctgtaga atatggggta atatccttgt tcatagtatt tttcactatc tgaggctgca
     5521 tctacggtca ataatgatat gttgggtata acaaataatg ataatattat tgatattatt
     5581 aatattaagc ttatttttct agtattcttc atattttttc acctgtttta ttttctaaga
     5641 tacattggaa aatatttaat tgattagtta ttagtttctt taaaccctac atataatcct
     5701 gtgcttttat cgccttcaaa ttttgtaaaa tatacactcc tattatttgc cttatattgt
     5761 tttttaagtt ctccaccctt aatacctaat tctatacaat agtcaaaatc ttttattacc
     5821 ttatctgctt ccttttcaaa aagatggttg aataattcaa tatatacatc tctgtgtaat
     5881 tcctttactg cttccggctc atgtattgta atgctataac cgtttaaata ccattcaggc
     5941 atatatatca ttataccact atcaataaat atgctacttt cttcataggc ttctttgctt
     6001 ttataaaaac ttaaaaatac taattcatct gtggcattat atccgatatc cggaaagcct
     6061 ttgcttttcc ccatgctatc cctcattact tttattacgt caattacttc cggtttagat
     6121 ggtggatata tctcataagt tacaccagta tatgtatctg ttatgactgc aatttcttca
     6181 tccttgggct tcattggctt tctcaagcct ttatctaggt acctcattac aatggcactt
     6241 ccttcgcccc tggtaagcgt ctttttaggc aggaaacttc catcgctaat gcctgttata
     6301 aagcccattg agtaggaata tagcacatgc tgtttatagt catcgcctat atcgtaataa
     6361 tcaggtattc ttaatttagc gtaacttgta tgattgggta tgggtgcttc ttcggttttt
     6421 aacgctgcct ttacgattat tcttgctgct tcttgcctta aaattggtct tctgtaatcg
     6481 gtaaattcct tttcgtctat gattttttct tccttagcca gctttatgta aggctctgcc
     6541 caatagtcta ttccttcctc taccttatgg cccattgcac gcaaggtcat tttcaaaaat
     6601 tcgtctactt ttaaggtatc ttccggaccg aacctgccat ctccgtatcc tgcaattatt
     6661 tctaataaat taagtttccc tacgtattcc ctgctccaat gtgttagcat gtctgtgaat
     6721 ttatttactg cattagtgac actctctatt ttgaaacttg ctaacttctc taataatggg
     6781 ttaattaagt cggataaagt agttgttatt ttgtttataa ttgttgtacc ttcgctatca
     6841 gcataaactg gtactatatt ggcaaatagt agagttgtta ttaaacataa tataaccgca
     6901 agtttaattt tcttattcat acgcttattg ccccctttta tttgtaatgt tataaaatac
     6961 atatcatgcc tttaatttat tctctactcg ggataaccga taattcaatc cttccattac
     7021 ctttagcgaa agctagtaat tcatcctgtt tagtatctac ctcaaggcat atatacttag
     7081 gaataacatt tctatcaata ttatttttta tgagttttcc ttcttgggat ataagtgcag
     7141 ctactcttat tttttccatg cgttctactt ttctttcttc aggtttttga gtaggtacaa
     7201 ataatatatc tacatactga tcagcttcta tccaccatcc gttagcctga tcgcctttaa
     7261 gttctaatgt gaatagtctg ttattatcat ttttaactgc tattttttcc attaactcgt
     7321 tctccttata tattttgctt tccaatatca tttcctttgc ttctatgtcc atttttgttt
     7381 ttttcccaat taatttacct gcattggtta ctgattttaa atgcgcaagc cctatattta
     7441 cttctctcat ctcaagcatg tcttcggtta tttctgtgtt cgccggtata tattcctttg
     7501 caaaaactac tttgaccttt gcttcgtact cagaacctgt ttttactata tatgtttgta
     7561 ctgcaagcag taccactgac aatattaccg ttattattat taatttagtc cttttcatag
     7621 gcttcctcac taaagatttt tattatttgt ctataatatt agttgtttat tggcagttct
     7681 gtatctacat gcaccttgat gtatataaca tctttgccta ttgcagacag gattaccctt
     7741 ctaaacagca tcggttttat aggaactttt ataactgagt gcatcgttgg ccttgtaata
     7801 ctattgcctt gcgagcatgt tatagggaag gttgtattta tatgcagata tgcctttaat
     7861 tcgtctatca ctacttctcc ttcagcaatg ctatttcctt caggtgtcat atctgctttt
     7921 aattttaggt tttttgctaa aatattttta taaattgtaa tttcagaatt tagtcttatt
     7981 gcagcgtcat tttttgcagc atcttcagta gatatagcat tatatgaggc taaattcgct
     8041 acatctacgg catcctttat tatgtttact ttgttatata atatatatct ttccatgact
     8101 acggcaaata aaggtaatat aataaatatt attaacccta gtaaagctat tacgtgggcc
     8161 gttgcactac ctttattgtt tttaatcatg tcctttactt ctatcatttc acaacctgcc
     8221 ttgatgttat tgtcctgtca tataccatcg tactggtagt cttttctgtc gtaaacattg
     8281 atttaaagct tctataatcg tatttcactt ctacttttaa gtttaattcc tcaccttttt
     8341 tcttagtaaa agttggtgtg ccggttatag ttatttcttt aaaacctaag cttgttaact
     8401 tattttgaag attgatttct tctgtaattg ttaacccgtt atcgttctcc atttttaata
     8461 gtgtctttct gcattctata tccattgcat ttttctttga cataggtacg aacatccata
     8521 caataccaac gataaggctt attattacta ctgtgattat agaaaagatt attatgttgt
     8581 ccatatcgac ctccatgagc atatatagag aacagttagc cagagaagtc cgaattacaa
     8641 aaaacttctc tggccttaga aaccattaaa tcgtatctat gaatactttg gttgttactc
     8701 tgctccacca atcctgtaaa tgacctgcta tgctttcgta caaggtatct aaagctgggt
     8761 acattactac cactgttatt attacccctg ctacaaggat gagaatatag cttactgcac
     8821 ttcctttgtt gtcttttaat gctgtttttg ctttttcttt acttgtcatc aaattgcttc
     8881 tcatttttaa ttctgttact attaattttt ctttaatgtt tttcatatta ataaaactcc
     8941 gtttctgtat taataatttt atttgcctaa aaactaaaca tttcttctat accggacaat
     9001 gcttccatta tcattggcat tgttaccatg agaacaatta taaatgcaaa agataatgct
     9061 gctgctatgc caatatatgt acgtctatct gtttctgcct ggatatagtt catatactta
     9121 ttgaacatca attcttcttg ctgttctaat agattgccct cacctcctgt ggctactcct
     9181 tgttttatag ctattactaa tgtatctgct tccagattat cgtactcgtt agatatttct
     9241 tgtattgcaa ggtctatatc agttgttagt tcataggttg ctgccatcct tatgaggctc
     9301 atttttattc cttcatcgtc cattatttga tatactattt ttatagcttc tggtatcgta
     9361 acacctgatg atatttggtt atgtaagaat ttatatattt tgtatacatg tctttgaaac
     9421 ttcttgttta tttctatttt attttgataa atcactaatt ccgttgttac atagcaggct
     9481 gtcgctgcta ttataggtcc taagatattt tttctataat ttgttagcat agctattaga
     9541 aatattaata acggtacaat gtatttgcag gcaagatata ttaatgctgc gtgcttgttt
     9601 ttatatcctg ctttttttac cttttccttg gctttagtat atatatctct ttctttatca
     9661 cctccactta ctttctccag ttccagtgtt gcttttcgcc ttatctttaa aaacaatgct
     9721 ttttcgctag tcctgcttcc ttttgcttcc cggctatacc tcccccgttt taatttaagt
     9781 tttagtagca aatttgatat aattatgccg tttaaaatta gcattattat actgaaaaca
     9841 atattgcttt ttgccagatt aattatttca ttcataaatt caggcgtgga gacttgtgac
     9901 ttcagtcatg agaggaaacg cccgtcctcc tttctgctaa taattatttt atgttacatt
     9961 aatttttaat atctaaactg tgtaattttc atagtaaggt atatgcctac tgcatacact
    10021 aacgaaaata ttgctaataa tattctccct tgttcagtat atagataata tgctctgcct
    10081 tccaaactgt attttaatat agctgttgct acaacaaata ccaggcccat tattatataa
    10141 attacaactc tatctgttaa agttgaaatt ttacgtctat ccatttcatt ttttatttta
    10201 tagaactgcg actccatatt agttaaaagt tttttagtat ctcccctgga cttaatgttt
    10261 tgttttatat ttataacgaa ctctgaaaat tgttcgttgt tcaccttgat ttgtaaaata
    10321 tctaatgcta tctcaggatc tattccactt tttatttgta taaccatttc agctatatat
    10381 gttctcagag gataatctaa agattctact gatttttcaa atgcgtaaaa tatatcatcc
    10441 tttacagaac accatctgtt taatattgtt ataaagccag ctaattgttg tgcagtcttt
    10501 tgcgagttta ttttgcctat catatctaaa ataactacgg ggatcattga tattattgca
    10561 cttattatta ttgatgttgg caaccaattt accatgcgat atgcaggttt tatagagagt
    10621 aagaatatta ttaacatgaa aataagtagt atatatgcgt ttatttgtat atatctaaat
    10681 ttcttgttta cgtttgtacg gtgtattaaa tttatttcta ttcgttctat aaatcctttt
    10741 tttgtatcaa gcttggttgt gtattttttg atttcatttt ctattaatct ttcttctaac
    10801 tttttgttca tattagttag ttttatatgt atgatttttt tatacacaat ttgctccgca
    10861 atgaggaaag ataaaactac tgttgatatt actaaaagtg cagatactat ataaaatatt
    10921 atttctctca tatctcttcg cccccaagtt tatcatttag gcgttcccgg aaattatttg
    10981 tttttaagtg ttttgtttta tcgtatttat ataattcatt gtatataaaa tcatctttcg
    11041 ctttatcata gcctagaatc tcaactatgt ttacgatttt gaatttctcc atgtgaatta
    11101 tgcagtcaat acttctacct attatgtctt ttacggtgtc ttcactttct ccaatgtttg
    11161 ccattttgct taatgtcaat aatctttcta agcaatcgta ggccttgcgt gaatgtgtag
    11221 ttgttaaaaa tctatggcca cttaacgcag ctcttatccc ttcccatgtt tcggcttcta
    11281 cactctcgcc tatcacataa gtatcaagac tcatggttaa accgtccttg atcaagtctc
    11341 ttagcgttac tgttcttcca ccttcacctt ctttttttac tctttgctct atgcaattgg
    11401 gtttctcagg gaatatctct gcatcctttt cacatatcag tactctgtcc aattccggca
    11461 tgttatttat tattgctttt agcaatgtcg ttttccctgc cgcccctttt cctgatataa
    11521 ctacatttgc atccgatact gcaagctttt taaggaatac ttctatttct tcgtcaagca
    11581 ttccttcctt ttgcaattca tctaatgtaa gacttttttc aggatgtttt cgtatgctta
    11641 tagcaggacc cgatatattt ctaggcggta ccgatacgtt aattcttaac cttttttggt
    11701 tatcggttac acggcaatgg ctatcatttt cgtttataat tccatagttc cttcttacga
    11761 gtagtttgca atacttatca aattgttctt cgctcccaaa atctatatct atcttttgcc
    11821 gcataccttt tcttgtgata gtaaactcat tatattttgt accatctata tcacttatgc
    11881 tttcgtcttc taagtactcc tgcaatggac catagccaaa catgaaatca aatacccttt
    11941 ttataaactt atctctttcc aatatgttgt atttattttt gtctatattt tttataattg
    12001 ctgtttctaa tactttttta tcaattatgc catttgctat gtcagctact aattttggac
    12061 tttcttgcaa tatattaaaa gtaatctctg tcactatgtc attcatatta gattcagcgg
    12121 ttctgtttgt tttaacttga cttccttcca gcaactgatt aagagttaga actgtcggca
    12181 cttatatttt catcccttct tgttaatcta tttttttaaa tttaaaattt tcttttgttt
    12241 tcagtttagg tgttagctcc agctgttcaa ttatttttat ataatccctt ttaactgctt
    12301 tctccattgt tttcgcataa cacttatttt tacttctgct tagtactctt gctttgctat
    12361 atcttacttt acctatataa gtgtcttctg ttatttcctt taactctgca tttgtttcat
    12421 gtattcctgc tatgtattcg taacctacaa attttgtttt atctagcctc attccttgct
    12481 tgtcctctaa aaacataagg taattattat attctctcaa ctctactgta ttaggctcta
    12541 ttgctgctat attaatatcg gaccttaata agcttgttat tgtgtatgca tcgtaaatgt
    12601 gtttgtttgc taataatatt gttatttcaa aattctgatt tgccttttct atgatgtctc
    12661 ctaaattctc aatcgtatag tattcaaagt tatctaggtc ataattgcct gtgatgatat
    12721 ataaattttt tagttcttcc ctttgtattg ctgcttttct tattaattca ccatttaaat
    12781 acttcctgtt tgaccattca attaaaatat ctagtcctgt ttttctctct aaaaggtcat
    12841 cagactcttt ttttatattt aaatataaat ctgctttagg acttaataaa tctacatcaa
    12901 gcaacaatat attttttcct gtttctttcg ctatccggta tgctaattcg catccaaatt
    12961 ccggattgcc ccatattgtt attattttgt tcattctgcc ccccttcctt tacttgagca
    13021 aaattaattc ttttttttat tataccatcc actttttgat gtcaaatgca tattacaatt
    13081 tattgtaata tctgtttgtc atgggggttt tgactatata ttatagtaat acctaaattt
    13141 atagccttgt gagatttggg gcaaagagta ttcccacaag gctataaata atttatgaaa
    13201 ctttagataa aatcttttct gcaatatcat cttcgtcaaa ctctgaagat ttataaataa
    13261 tatcgcagaa ttttttaagc gtgaaatcag atacacttcc tatgccttta tctaatacca
    13321 ctccgattac gttaaacttc atttcatttt ttgtttttaa aaattctttc aaaaaatcgt
    13381 ctgaaatgtc gcattcaccg tcggttataa atactagatc ggcatatttg tacttatctg
    13441 tttctaataa cttcttagct tccagtaatg gtgtttcaaa atcagtgcca ccgtctagaa
    13501 agttacatgc cattttcatt acagcttcca ggctataatt ttctttattg atttcattgg
    13561 taacacattt tcctttactg gaaaaatgaa tgctggcaaa atttttgttg ttagataatg
    13621 ctacctctaa taaggaacaa catatcgcct tgctccaaaa gtctttacca ctattcatcg
    13681 acccggattc atcgcaacaa attaccatgt ttcctgaacc cttgccaacc ttttctctct
    13741 ttttgaactg ctttaactgt ttcccggcat acttctttaa aaatacaagt ttgctatgag
    13801 gattagataa cattgcaaat tcacttgata gcaccctgtt taaatcattg cccagctcta
    13861 cactataccg ttcgccaata tcataagtgt atgaatcttt tagtttgctt cttgcaagtc
    13921 tgcggtaacg acctactaac tttgccattt cgcgaaacct attgttttta cgtacttttt
    13981 caataaggtc cttcttatat aatttaggtt ttcaaagatc taattatttg taaagtatta
    14041 taatatatat gcacaaagcc gtttgatctt ggcaggtgat ctttgcacag tcataaaata
    14101 tttacggata gcctgcacaa tgtctgctat tgattcgaac aagtggttat gtgttacatt
    14161 ctttcggatg tctttccaaa ggtgttcgat tgaattcaaa tcagaactgt aagggggtag
    14221 atactttaaa gttatatttt ttaatgagtc caataactcc ataacactgt tggtatgatg
    14281 atatcttaca ttatcaagga gcagtatcac tttcttgtta acataatgtg ccgacagctt
    14341 attttaaaaa atccttgaag ctatcagagt tgccagcttc cgaagttgaa taatgcacct
    14401 tgccgtcaat ggggtttacc gctccaaaca taacgattct ttccctggag ccggaactgt
    14461 ccgtcggtat gattggctga ttacccttaa gcgaccattt taatattgta gtaggttcat
    14521 ccgtaattat cgcctcatct tcatagagta ttacggtatc atcatcggaa ttttcaagaa
    14581 tatcctgcaa ttctttctta tattgatcct gcagctcctt cttggcattc cggctctgct
    14641 ttttaggtct ttgcagagtg aacccaagtt tgtggactaa atcatacaag cttgaagcct
    14701 tataattgat gccccattgc ttgcttatcc acaattttaa taggggaatg ttccagttgc
    14761 ttttattgaa tccgcattcc cggggagatt ttagtaaggc ctctttcaca gcttgcctct
    14821 gctctggaga taaataacta gttcggttgc tgtgccgggc atcaataaga ccagctatac
    14881 catattcgtt ataccggtgc acccacttac ggacattggt atctgccata tgaatatcct
    14941 tggctacttc tatgcttggt ttaccatccc aagtctgtaa tacagcaagc attcttgttc
    15001 gaagttttgc atcattacag tccttgtaag aagcttcaag ttcttcatag gaacagtgtt
    15061 ttttatttat ttcaagcttc cttctactca taatatcaga gcctttctaa aaaatgttct
    15121 gatattatga taacctatag tggtgtcggt attatacagg taatataatg taattacatt
    15181 tatgaaaacc taaactatat agtttcggtt gccatgtatt taggatcatt atcgccccat
    15241 gcatctaaaa tatcttttat ttcttctgtt ttattaattg cttttttcag cgcagctgat
    15301 attttttgtt ctatttgctc tttatttttt gcgctgtttt ggtcaactat cttttccagg
    15361 tactctatct gtttcttttg tttttctaaa tcttttgctg tagctattgc ttgctgtatc
    15421 attatttcag attgctccag ctcccctttc tctgaaaact cttttgcttt atctaataaa
    15481 tcttgcaatt ctccttgttt ctctttactt tgtaaaacct ttttttccat tacatccagt
    15541 gtctttttct caccggacac atcctttaac aattcgtcta aattatcaaa tatgtttact
    15601 ataaactcct ttgttgcttc aatactttcc gtatcacaac cttctgttaa ttgcttcaat
    15661 acatgatatt catcatcttg cattgttttt attatgatgt gcttattaaa tttttgagcc
    15721 ggcaaactta actccgactc atctttgatt tcaggcttta cattataaaa tgcattaaaa
    15781 atatcttgtg ttaaagccgg aaaggttttt aatttttcct ggcccagatg ttctatattt
    15841 tttagcacac cattatcttg ttctacttct tttctctttt cttcatagac cattttatct
    15901 atcattgaat gtttaataac attatcattg cctatatcgc tactggcttt tagtatgtta
    15961 tcagctgctt ttagccagcc tggacttaat acactcctga acaattcctc aaaattagga
    16021 ttgactttca agtgttttca tctcctttac tgttttattt ttttgtattt gtcgggaaaa
    16081 tataagaaag gggattaaat tgaacttcgt agttttcccc cttcttttat gtgacaaatt
    16141 tcgactgttt ttacctatcc atcatcattt ttgtttcttt taatggtagt tgtgaaaacc
    16201 ctaatgcaga gtttattttc atattaagtt cttctaattc atctattgac ttgatataca
    16261 gttctctatc ggaatctgac cttatgtttt tgtcgtaccc caacatgata tcgtatatac
    16321 ggactatttc cttcctaaat tttttgcaaa ggaccatctt ttctctttct tccagcccct
    16381 tattgattgt ttcaacatta tgattgtata agtccctagc tattgccatg gttgcatcta
    16441 tagattcttt cataggattt tttatatttt gttcaattat ttcctggacc atcggtatat
    16501 ctgatggcat atcccagaaa tagtttttta agtatgccaa atcttctatt gcaacagcat
    16561 ttcttttatt gaggtacgca acagcttgca gcaaggtagt acagtttaaa taccttctgt
    16621 ctgttacatg aatacctttt gctctaaggt ttattaatat tttttctagt aattccttaa
    16681 tgggtgtcgg aacttctaca aacaataatt ccttttgcat tttttctaac tcagactttg
    16741 gtatagtggc attaaccata aggtttttgt ttaacaattt ctctcttttc ctttgcataa
    16801 ccacattcaa agccttttct ctgttagact taccggttat ataatcaact ttaactttga
    16861 tttcaaaccg atctattagt gcagcaaaaa cacgttcttc aggatctctt gtatcgggta
    16921 tttcgtttga ggcaccaata aatgtgaaag ccggtatgtc atatgtcatg ccatagtttg
    16981 tatattctct ctcattcatc gcttttaaca aagagtttaa tacggaccta gctttgaatg
    17041 tttcttccaa aactacaaca tcagcttcag gtaacatatt tgatgtatct attcttttgt
    17101 tgcctttagc aagttctgta atgtctaata cgccaaacag ttcttcagca ttaaattgtt
    17161 catgcaattg tgcttcgaag tgttttgcgc catcaattcg gtttaccaga tttctaatag
    17221 tatctgattt gttattgccc ggcagaccaa gcaaaaatat attcatcctg gctagcattg
    17281 caagtgctaa atactctatt aattcctctt tttctatggc taatgttgca acatcagcca
    17341 ttactgcctt tagtttatca actagctcaa aactaaaatt gaatttttta ctttgcattg
    17401 attacctctc cttttttaaa aaaaatttta gaggtgggct ttttccacct ctaattctag
    17461 ttttgtattt gtttttcttg aatctaggca gctacaaaac ctatttgacc atctagaggc
    17521 tttgtacctg tcttaaaatt tgatgtttct ctaagcctca tttgtagttc cgtggccaga
    17581 aattccacat tttcaaattc atcattgagt ttacttaaac ttttattgaa aaggagctca
    17641 tattttccct ttttatcatt aagttctttg attttttcca gccatctgcc tataatcttt
    17701 tccgatttgc t
//
LOCUS       DAOSDD010000075        17671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1301_length_17671_cov_5.261637, whole genome shotgun sequence.
ACCESSION   DAOSDD010000075 DAOSDD010000000
VERSION     DAOSDD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17671
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1301_length_17671_cov_5.261637"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..402)
                     /locus_tag="PK733_08440"
     CDS             complement(169..402)
                     /locus_tag="PK733_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10607.1"
                     /translation="MDDFVRNSLADTILHSNMSFIGDKLTIYNRKFYKGSAVKLMGTG
                     AEVAFTLAGDELSIVQGLERNNAGAALIELYVV"
     gene            681..1157
                     /locus_tag="PK733_08445"
     CDS             681..1157
                     /locus_tag="PK733_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ10608.1"
                     /translation="MYFARKHKGHKGIAGTPVEHYHGTLVHDHDITFYCYGDNHQECL
                     IHVLRHLKDTMENEPNLKWSQRMRKLLQEMIHYRKGLSEESPYPDPDKVKEFENRYIE
                     ILELAKEEYEYEPPSNYYKDGYNLYKRLDEYRNSHLLFLHDIRNPTNNNLCERKTS"
     gene            complement(1227..1793)
                     /locus_tag="PK733_08450"
     CDS             complement(1227..1793)
                     /locus_tag="PK733_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10609.1"
                     /translation="MTWGFVEIVNIRTRKRRNLNMLEQQIYNCSIIEAEDKDIERVVE
                     IAINAWQPIFEGYKNELGDIIYNCEYPDWKKEKARQIYESFKPQSGARVLLAEINGVI
                     AGFITYYVDKSNSSGIICNNAVDPKFQGKGIAGNMYKYVIEEMKKHKLKYVKVTTGGD
                     AAHLPARKAYEKAGFKKNIPEVNYYMEI"
     gene            complement(1759..3957)
                     /locus_tag="PK733_08455"
     CDS             complement(1759..3957)
                     /locus_tag="PK733_08455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ10610.1"
                     /translation="MKITNENQHYCTNMHTPEGRYIPTEQLRFAFAGDSIPWGEGLLD
                     ESFVSSVDDFIRNSLADTILHSNMSFIGDKLTIFNRKFYKGNAVKLMGTGAEVAFTLA
                     GDELSIVQGLERNNAGAALIELYVDGVLHDRFSNYNELPCGSETKIFTGDGQTVKFDL
                     ERCFTYNHTVTIDGVQVTGKINDSGYGAAFDSTQDYMIIRKYGTNPETGEIEVHHVIW
                     FEHAPADGTNIAAAFSYGENISYTKSTVGEVSANINSPLESCYGDDVVAFDPAKPSGI
                     SSGLDFRYTDERAVKTWKFDTYANRTFKFKIKNLDSRASSGTPYFIINFATNRFHHFM
                     NAGIGGWTTEKFYTHNLLNFNTMMKFKPDIVFLAFGTNDDWEAGKFVATRQITGVTEA
                     EIRRYHTLFLKSCKYSDDGNYVIETANMVIRETGENSLTIDNVGATLTDIKKGDILAI
                     GDYHGDNRTVAIRIIDTWDISTNTATFKEKLIPENIEGITSLSELAGKTVRVKRIDRF
                     INTLSDCIDGIRSYNPRARIGLVDTGLSNYYERVLMGYTEKIIELAHLKKCFHVGNYK
                     HIQNWQYSQEKDANAYIGYKSLDISTGAAEYPIVDSSNFDIHEAYGTMLRNFSMKVNG
                     VERLNRGCFIDGGYAVAFKDSSPANELTICNWNNRTKNTSIEYRYIPARLKFTFDIPV
                     KGDIINIKFTSVKWSSDDCHIGKGSGGDAMYSKAVIDSLVDMGICRNSEY"
     gene            complement(3964..5025)
                     /locus_tag="PK733_08460"
     CDS             complement(3964..5025)
                     /locus_tag="PK733_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10611.1"
                     /translation="MVIFTEKEELKNGEWLTIKAVKSPADKYEDRIFSYINPKMPWRQ
                     DTYKRVNGDVADQCKDVFFIGEIGGEIVSLMWYTVSDDVGTYGMVSTSEKHRNKGICG
                     CIMKYCIQFMDSEPGLQAVYLAVSNPMAYKIYEKNGWQPYNNGTLNDTCIMRRLINCN
                     IDEKEFDDNYYKKQQNDGSGIVIRRVIRGDLPKLEALYNLVESTVMIKDYNASIYKGT
                     AVECQIIDLINKMETGEGQFYCIENGLGRIVGAATLYNKANGYSTEGSNTEGSNTKSS
                     TTNLTDNERSKKILDFFVHPNYEEYGKSLVSKIIECNDGCSKNKIVSYIAPNDKSKAD
                     ILYDEKLKAKIAKIVTKKE"
     gene            complement(5040..5810)
                     /locus_tag="PK733_08465"
     CDS             complement(5040..5810)
                     /locus_tag="PK733_08465"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10612.1"
                     /translation="MKKIDAHQHYYSNPHTLEDLIKRSKDAGIVKICLSGAGKPFGQP
                     GNDEVKQAFNIYPDMIVGFGFIRLGIDSWHLVDELYEQGFRGLKTISPRKNYSDKEYY
                     PIYKRAEELNMPILFHTGIKIFNPLDVKHDVACERMQPIFLDAIARAFPKLKIIGAHL
                     GVPWYNEACMVARANPNVFFDLSGAINLLYELKPWFFETLTYWEMAKEKFVFGVDDHY
                     ERIPEAVNKFNVLMDKLNFSQELREKIFYGNMEKILGL"
     gene            complement(5877..7163)
                     /locus_tag="PK733_08470"
     CDS             complement(5877..7163)
                     /locus_tag="PK733_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10613.1"
                     /translation="MEFKIALIGAGSRGTQYSSHALKHGGTKIVAVVDPNEFRRNIAA
                     EKFNIDSKNCFSSVDELLENRVEFDGAINGTMDNMHVKTALPLLRRGKCVMLEKPMCL
                     NKEELFELYNTSVKYNAKLMVCHVLRYTPFYSTIKQRVVNGEIGEIKHIYASESVANY
                     HMAAAFIRGKWGNSVECGSKILMAKSCHDMDLLTWFKSGVRPVKVSSMGSLFEYKAEK
                     APEGAGKKCIVDCEIESECPYSANLNYLKRDDFDVYSWRAIIEKGTDLSGKTIPFEEK
                     VKYLKSDDRFGRCVYYLDNDQYDTQNVSVQFEDGSVATFGLDAGTPYGERYIRLEGTK
                     GMIIGQFMKQQFIIRKPKLDLDERYTEEVVDINCSLDGHGGGDTRIISDFINLALGKE
                     TSISCTSIEDSMYGHLIGFNADIAVKEERVVRIEKL"
     gene            complement(7502..8272)
                     /locus_tag="PK733_08475"
     CDS             complement(7502..8272)
                     /locus_tag="PK733_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10614.1"
                     /translation="MKIIDAHQHYYSKTHTLEDLIKRSKDVGIYKVCLSACGEQFNQP
                     GNDAVKQTFNLYPDMIVGFGYVRLGIDPPSLVDHLHDEGFKGLKVINPKKNYSDKEYY
                     FVYEKAARYKMPILFHTGIMGRGSGNGDFDTACERMRPIFLDGIARAFPNLNIIGAHI
                     GVPWYNEACMVARANPNVYFDLSGAINILYEMEMCFFDTLTYWNMAKEKFIFGVDDHY
                     EGIQGAVNKFNILMDKLSFSRELREKIFHGNMERILGL"
     gene            complement(8458..9408)
                     /locus_tag="PK733_08480"
     CDS             complement(8458..9408)
                     /locus_tag="PK733_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ10615.1"
                     /translation="MARYTKVSSIGARPVLSDIDIDYSQTVEKVISYWKHELDQVLPD
                     KPDIIVLPEGCDRPLNYPKLEKEKTEYYKNRGNQMLEFFSNIALNNNCYIAYSAIREA
                     GDGTLRNSTQIIDRTGKVVGIYNKNYVVIEETTESGILCGKDAPIIDCDFGRVACAIC
                     FDLNFDELRLRYVKAKPDIILFSSMYHGGLMQNYWAYSCRSYFAGCVAGLQNTIISPV
                     GEIIASSTNYHDFVTAMVNLDYCVVHLDYNWEKLKRIKAKYGPKVKLTDPGYLGSVML
                     SSETDEFTISDITKEFEIELLDDYMRRALAHNNNKMNVEP"
     gene            complement(9588..10361)
                     /locus_tag="PK733_08485"
     CDS             complement(9588..10361)
                     /locus_tag="PK733_08485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ10616.1"
                     /translation="MQIIDAHQHYYPEPQTIEELVERSERAGITKICLSGLGDYSCDK
                     YNEDVKKAFEKYPNTIIGFGFIRLGIDTWHLVDELYEQGFRGLKTISPRKNYSDKEYY
                     PIYKRAEELKMPILFHTGMKISNPADIKHDVACERMRPIYLDSIARAFPKLNIIGAHL
                     GVPWYDEACAVAAFNRKNVLYDLSGAINLLYEFEPWFFKTLSHWESAKERFVFGVDDH
                     YERIPGAIDKFNALFDKLEFSEELREKIFYGNMAKILGL"
     gene            complement(10392..12542)
                     /locus_tag="PK733_08490"
     CDS             complement(10392..12542)
                     /locus_tag="PK733_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10617.1"
                     /translation="MKNSNLKRGYNSMNLNENQYLSIIEYDSYINVDNDCLNIKIDRE
                     TGNIVEFLLKEKNIDILDGEKGYIALHYKTAGQLCFTPDDVKMKSYSINKSDKSNVLY
                     INLASCKCDTELNIELKMEDGNIRLNCTPITSKPKMSEVEVVYVLPFFKANITGFAPY
                     SNADLLPEEIDHKLLIYGGDQFRGELGDSISLPLAGFYNKNENYGITVIQPVDIYKPQ
                     VQYYYIRENAPLSFVVKYCNLRLSKYDSPKTSMIFSIHEGDWRCSLKAIYDLYPEYFN
                     VNNNSIYETEGTMLCSTLQPEENIKNWALQQGLKWQEIHANIFPYYGLYAPEEEEWND
                     ISHTYDISKLNLKRLVDAEYWEIDNCMMKRSNNKKITRKDVNDYIEMLHKYGVGAFIY
                     INAVILNKYEKVKYKDSEAIGLDGLPCFKNYYLNNPMNPDPGLSWGQNIIYQTEKILN
                     YFPECDGLFFDELHFRHFDMNHDDGITAVNDRPSYMIGFAIEKISKLIYEKIKNRGKS
                     IWGNGPTSLEVMRYIDGLMAECSWEWLGTVQYYALNKPIILLLRRTEKITELEEALKN
                     AIICGSQMHVEWKPDDKKGTKETAKMLPADDEERLALLKGYGKILELLKKRSWYLKAH
                     CLNISDDNYKGNIFKIPSGGYVVTLINKTHGYCYNDIGIELLLEDDFTVNKIYGVDPE
                     NNKESEIEWKKDENMVNKISIKLKIKNSIAIVVE"
     gene            complement(12706..14379)
                     /locus_tag="PK733_08495"
     CDS             complement(12706..14379)
                     /locus_tag="PK733_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10618.1"
                     /translation="MKKILAVILTALMLLSMVACSSGKNSSNQADTTTKAAPEVKEKE
                     PVELNIITTIAEDDEKFYNTPVYKEFTKQSGVKLTSTYVDKTKFDLLIAGGDTTDLLA
                     CYHAENYKILASGGLVLKLNDLIDKYCTNLKKPENDIMWTILKDKYDNGDGSIYGIIN
                     SIGPAGGNPRATNLGTYTIWDYYKEIGAPELNDFGDFVDALEQIVKLHPKTADGKPIY
                     AFSLYNVENQLYPFYGPLLSVVGVSTYNPYIALKIEDNSLSYMFLDEKGPYWLAAKYM
                     NDAYNRGILDPDSFIQTSADFSTKMENGQILNHYYAMSTKGWEIIPLKGARMTENIFD
                     IGGNCICIASTCKDKIAAIKLLDYLNSPEAARLIYSGIKGEHWDYNDKGEACLTEKTM
                     ALINAGGDEWAATGVDNYTLYSIVGVARGVKCADGSITALDRTKSYFTAGLNENGKDY
                     IKFYSDRDKANYSYPNEVLYARGVKDGSNRNTAILKGFGTAPDDIARIDSALLEIAIN
                     SVPKLVMAAPKDFETVKANVLADFKSAGVQKSYEYWQSRWKVLADKFMK"
     gene            complement(14488..15378)
                     /locus_tag="PK733_08500"
     CDS             complement(14488..15378)
                     /locus_tag="PK733_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10619.1"
                     /translation="MIGKKSFGSKLFDISNILIVIMFSIFCVYPFYYMLCYSLSDPVL
                     ATKGITLWVRGFTFNNYINVIKLKEIPQAALVSVTRTILGSSVTLFCSGFFAYLMTQQ
                     KMYFRKFIYRFVILTMYVGGGLIPTYLVMRYLGLRNNFLVYILPGAVSAYYVILIKTF
                     IEQLPQSLEESAKIEGAGILMCWFRIIMPLSKPILATIVVFTMVGQWNSWFDAMIYMT
                     RNNLKPLQLILYEYLQEAQRLSDRIREGIESGVKVDVSLTPDAIRMTVTAVITIPILL
                     VYPFMQRYFVKGIMIGAVKG"
     gene            complement(15375..16304)
                     /locus_tag="PK733_08505"
     CDS             complement(15375..16304)
                     /locus_tag="PK733_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10620.1"
                     /translation="MKIGLDSTLRKRKISIEGIKLTLLALPFVIFVFVFCYLPLFGWS
                     FAFFDYKPGLSLSQAEYVGLKYFKYIFSYNKDMIRVLKNTLAMSFMSLATSVLPVIFA
                     ILLNELRNLRFKKFIQSFVTLPHFISWVIAYSLCFAIFSTNGMYNNLMQVLNITDKPN
                     TVLSNSNYVWTFMTTLDIWKGLGWGTIIYLAAIAGIDAELYEAAIIDGAGRFRCIWHI
                     TVPNLMETYIVLLLLSVGNLLSIGLDKYLNFKNPLNASKIEALDLYIYRIGLMTHDYS
                     FATAIGIAKSLVSIVLLFTVNKLARKVRGESII"
     gene            complement(16647..>17671)
                     /locus_tag="PK733_08510"
     CDS             complement(16647..>17671)
                     /locus_tag="PK733_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ10621.1"
                     /translation="IITGSNKGQYTDTGTESQMIWETMPDAKLIITSDILLQEYFSEL
                     TEISQTLLCIIIPGFILACILFYLWLGKAFKPLITLKRSMRRTGQGSCDELLPATGYD
                     ELSMLIMSYNDMLKNMQKLKKQNEIVEQEKREGELKALRNQINPHFLFNTLSTIKWMC
                     LIEGNRNAADCITMLCGIISPMFKSSTPVCTLCEELETIDLYINIMNTRLDDGIIYEK
                     RVDESLLDTKILRLIIQPLVENAITHGFEKSLGQGNIILSAFKQNNAMIICVDDNGSG
                     MSDEDIAQLNQRMRNGNDSNGIGLMNTNRRLRLQYGAGYGVHVERGHVCGLRVTITLP
                     LDEQPA"
BASE COUNT         5319 a         3692 c         2635 g         6025 t
ORIGIN      
        1 taaatttcaa ccttgcagga atataacgat attctatgct tgtatttttt gtacgattat
       61 tccagttaca aatagtaagt tcatttgcag gagatgaatc cttaaaagcc actgcgtagc
      121 caccatctat aaaacaacct ctatttaatc tttcaacgcc attaactttc acacaacata
      181 aagctctatt aaagcagcac cagcattatt tctttcaagc ccctgtacaa tacttaattc
      241 atcacctgcg agtgtaaagg ccacttctgc acctgtcccc atgagtttta cggcgcttcc
      301 cttataaaat tttctattat atattgttaa tttatctcca ataaacgaca tattactatg
      361 tagtattgta tctgccagac tattcctaac aaaatcatcc acacttgaaa caaactttca
      421 tcatttggtg caatatagga aattatatta tcctctgaac atccatcatc acattcaatt
      481 atttggctta ctacggtgaa ttacagattt caaagggtat gatcaacgga cttagcaaag
      541 aattctccac caagacggag gaagaacaga aaaagcgttt gccgatcttc tgctctcacc
      601 tgtgataaac gcgatttcac aaatgccagg gtcaacggta aaacgttcag attgccatat
      661 gcgccacacc tgccataacc ctgtatttcg ccagaaaaca caaagggcat aaaggcatag
      721 ccggaactcc ggtcgaacat taccacggta cactggtgca tgaccacgat attacattct
      781 actgttatgg ggacaatcac caagagtgcc taatccatgt actccgtcat ctgaaggaca
      841 ccatggagaa cgagccaaat ctcaaatgga gccaaaggat gcggaaattg ttgcaggaga
      901 tgatccacta taggaaaggt ctttcagagg aaagccccta tcctgacccc gataaagtga
      961 aagagttcga gaacaggtac attgaaatat tggagctagc gaaggaagag tacgaatacg
     1021 agccgccaag caattattac aaggatgggt ataacctcta taaaaggctg gatgagtata
     1081 gaaatagtca tctgctgttt ttgcacgaca taagaaaccc aacaaacaat aacctatgcg
     1141 agcgcaaaac ttcttgacca cagtactttc agccaaatta tataatgctt caagcttagg
     1201 aaaatcgcat cgtacaaatt ttacatttat atttccatat aataattaac ctcaggtata
     1261 ttttttttga atcctgcttt ttcatatgct tttctagcag gtaagtgtgc tgcatctcct
     1321 cctgttgtca ccttaacata cttcaattta tgctttttca tttcctctat tacatattta
     1381 tacatgtttc ctgctatacc tttgccttga aattttggat cgactgcatt attgcaaatt
     1441 attccagaac tattagattt gtcaacgtaa tatgtaataa atcctgcaat tacgccattt
     1501 atttctgcta aaaggactct agcaccactt tggggtttga agctttcata tatctgcctt
     1561 gctttttctt ttttccaatc cggatattcg caattatata taatatctcc cagttcattc
     1621 ttgtagccct caaaaatagg ttgccatgca ttaatagcga tttcaacaac tctttcaata
     1681 tccttgtctt ctgcttcaat aatagagcaa ttataaattt gttgttctag catattcaaa
     1741 ttcctcctct tcctagttct aatattcact atttctacaa atccccatgt caacaagtga
     1801 gtcgataact gccttactat acatggcatc cccgccgctt cccttgccta tatgacaatc
     1861 atcgctgctc catttaactg atgtaaattt aatattaatt atatcacctt ttacgggtat
     1921 atcaaaagta aatttcaacc ttgcaggaat ataacgatat tctatgcttg tattttttgt
     1981 acggttattc cagttacaaa ttgtaagttc atttgcagga gatgaatcct taaaagccac
     2041 tgcataacct ccatctataa aacaacctct atttaatctt tcaacgccat taactttcat
     2101 agagaaattt ctcagcattg ttccataagc ttcatgaata tcaaaattag atgaatcaac
     2161 aatgggatat tctgccgctc cggtagaaat atcaaggctc ttgtaaccta tgtatgcatt
     2221 tgcatctttc tcctgtgaat attgccaatt ttgaatatgc ttatagttgc ccacatgaaa
     2281 acattttttt aaatgagcaa gctcaatgat tttttctgta tatcccatca acactctttc
     2341 ataataattg gacaggccgg tatcaacaag accaatccgg gctctgggat tatagcttct
     2401 tattccatca atacagtcac ttaatgtatt aataaatcta tcgatccttt ttaccctaac
     2461 agttttaccg gcaagttcac tcaaggaagt aatgccttct atattttcag gaattaattt
     2521 ttctttaaag gttgctgtat ttgttgaaat atcccatgta tcaataattc taattgcaac
     2581 tgttctatta tcaccatgat aatctccaat ggcaagaata tcgccttttt tgatatctgt
     2641 aagcgttgcc cctacgttat caatagtcag actgttttct cccgtttctc ttattaccat
     2701 gtttgcagtc tctataacat aatttccatc atcagaatat ttacaagact ttagaaatag
     2761 tgtatgatat cttcgtattt cagcctctgt aactccggta atttggcggg tcgcaacaaa
     2821 ttttccggct tcccagtcat catttgtgcc aaatgctaaa aatacaatgt ccggcttaaa
     2881 cttcatcatt gtattgaaat ttaataagtt atgcgtataa aatttttcag ttgtccaacc
     2941 ccctattccc gcattcataa agtggtgaaa acggtttgtc gcaaagttta ttataaaata
     3001 tggagttcct gaagatgctc tggagtcaag attcttaatc ttaaatttga aagttctgtt
     3061 tgcataagta tcaaatttcc acgtctttac tgccctttca tcggtatacc taaagtccag
     3121 cccactacta atgccagaag gctttgcagg atcaaaagcc actacatcat ctccatagca
     3181 gctctctaaa ggagaattta tattagccga tacttctcct actgtagatt ttgtataact
     3241 gatattttca ccataactaa aagcagcagc tatatttgtg ccatccgcag gagcatgttc
     3301 aaaccatatt acatgatgca cctcaatttc tccagtttcc ggatttgtgc catattttct
     3361 aataatcata tagtcctgcg tgctgtcaaa tgcagctcca taacctgaat cattaatctt
     3421 acctgtcact tgtacaccat ctatggtaac agtatgattg taagtaaagc aacgttcgag
     3481 gtcaaatttt acagtttgac catcacctgt aaatatttta gtttcacttc cacaaggcag
     3541 ctcattgtaa tttgaaaatc tatcatgtaa aacaccatca acataaagct ctattaaagc
     3601 agcaccggca ttatttctct caagcccctg tacaatactt aattcatcac ctgcgagtgt
     3661 aaaggccact tctgcacctg tccccattag tttcacggcg tttcctttat agaatttcct
     3721 gttgaatatt gttaatttat caccaataaa cgacatatta ctatgtagta ttgtatctgc
     3781 tagactattc ctgataaaat catccacact tgaaacaaag ctttcatcaa gcagtccttc
     3841 tccccagggt atactgtctc cggcaaaggc aaatcgcaac tgttcagttg gaatatacct
     3901 gccttccggt gtatgcatgt tggtacaata atgttgattc tcattagtaa ttttcatatc
     3961 ttattattcc tttttagtaa caattttcgc aatttttgct ttgagtttct catcatataa
     4021 aatatcagct ttgcttttat catttggtgc aatataggaa actatcttat tttttgaaca
     4081 tccatcatta cattcaatta ttttacttac taatgatttt ccatattcct cataatttgg
     4141 atgtacaaaa aaatccagta tttttttact tctttcgtta tcggttagat tagttgtact
     4201 actttttgta ttactacctt cagtattact cccttcagta ctgtacccat ttgctttatt
     4261 ataaagggtt gcagcaccca ctatacgtcc taaaccattt tcaatacaat aaaactgtcc
     4321 ttctcctgtc tccatcttat ttatcaaatc gatgatttgg cattctacag ccgtaccttt
     4381 ataaattgat gcattataat cttttatcat aacagtactt tcaaccaggt tatataatgc
     4441 ttctagttta ggtaagtcac cgcggataac ccttcggata acaatcccac tcccatcatt
     4501 ctgttgcttt ttataataat tatcatcaaa ttctttttca tcaatattgc aatttatcaa
     4561 tcttctcata atgcacgtat cattcaaggt tccattattg tatggctgcc atccgttttt
     4621 ttcatatatt ttatatgcca tcggattgga tactgcaaga tatacagctt gtaaacccgg
     4681 ctcactatcc atgaattgta tgcaatactt cataatgcat ccgcatatgc ctttatttct
     4741 atgcttttca gaagtagata ccatgccata tgtaccaaca tcatcggata ccgtatacca
     4801 catcagagaa acaatttctc cacctatttc tcctataaaa aacacatctt tacattgatc
     4861 ggcaacatca ccgtttactc tcttatatgt atcctgtctc caaggcattt tgggatttat
     4921 gtaagagaat atcctgtctt catatttatc tgcaggagat tttactgctt ttattgtcag
     4981 ccattctcca ttttttaact cttctttttc tgtgaatata accattatca tttcacctct
     5041 tataatccaa gtatcttttc catatttcca taaaatatct tttcccttaa ctcctgtgaa
     5101 aaatttaatt tgtccattag aacattaaac ttgttgacag cttcaggtat tctctcgtaa
     5161 tggtcatcta ctccaaaaac aaacttttcc tttgccattt cccaatatgt taaggtttca
     5221 aagaaccaag gtttcaactc atataaaaga ttaattgcac cgctcaaatc aaaaaacaca
     5281 tttgggtttg cccttgcaac catgcaagct tcattatacc aaggtacacc cagatgtgct
     5341 ccaattattt ttaatttagg gaatgctctg gcaatagcgt caagaaaaat cggctgcatt
     5401 ctttcacagg caacatcatg ttttacatcc agtggattaa atatctttat gccggtatga
     5461 aagaggatag gcatatttag ttcttcagcc cttttatata tagggtaata ttctttatca
     5521 ctataatttt ttctcggact gattgttttc aaccctctaa aaccctgttc atacagttca
     5581 tctacaaggt gccatgaatc aattccaagg cgtataaacc caaacccgac tatcatgtca
     5641 ggataaatgt tgaaagcctg ctttacttca tcatttcccg gttgaccaaa tggttttcct
     5701 gcacctgata agcaaatttt aacaataccg gcgtcttttg atcttttaat caagtcttcc
     5761 aaagtatgtg gatttgagta gtaatgctga tgtgcatcaa tttttttcat ataaaacccc
     5821 tccaatttag ttattattat ttatttgaaa caaccgtata ttcatttgcg attattttat
     5881 aatttttcta ttcttacaac tcgctcctct ttaactgcaa tgtcggcatt aaagccaatc
     5941 aaatgaccgt acatcgaatc ttcaatgctt gtacaggaga ttgatgtttc ctttcccaat
     6001 gccaggttta taaagtcact gattatcctt gtgtctcctc caccatgacc gtcaagagaa
     6061 caattgatat ccacaacctc ttcagtgtat ctctcatcga gatcaagctt gggttttcgt
     6121 ataataaatt gctgtttcat aaactgtcca attatcatac cttttgtacc ctcaagtctt
     6181 atgtaacgtt caccatatgg agttccagcg tctaagccaa atgtcgcaac acttccatct
     6241 tcaaactgca cactgacatt ctgtgtgtca tactggtcat tgtcaagata atatacacat
     6301 cgtccaaagc gatcgtcgga ttttaagtac ttgacctttt cctcaaaagg aattgttttt
     6361 cctgataaat ctgtaccctt ttctataatg gcacgccatg agtatacatc gaaatcatct
     6421 cttttcaagt aattgagatt agcggaatat gggcactccg attcgatttc gcaatctaca
     6481 atgcattttt ttccagcacc ctccggcgct ttttctgctt tgtattcaaa aaggcttccc
     6541 atgcttgata cttttaccgg tcttacaccg cttttaaacc atgtaagaag gtccatatca
     6601 tgacagcttt tcgccattag tattttagaa ccgcattcaa cggaattacc ccattttcca
     6661 cgtatgaatg ctgctgccat atggtagttt gcaacagact ctgaagcata aatatgctta
     6721 atttctccta tttcaccgtt tacaacacgc tgctttattg tgctatagaa tggagtatat
     6781 ctgagcacat gacataccat taattttgca ttgtatttta ctgatgtatt gtacaactcg
     6841 aaaagttctt ccttattgag gcacatgggc ttttcaagca ttacacactt gccccttctt
     6901 aaaagaggaa gagcagtttt tacatgcatg ttgtccatcg taccgtttat tgccccgtcg
     6961 aattcgactc tgttctctaa aagttcatca acactggaaa aacagttttt ggaatcaata
     7021 ttaaattttt ctgccgcaat attcctgcga aattcgtttg gatcaacaac tgcaactatc
     7081 tttgtgccgc catgctttaa tgcatgactt gaatattgcg tgcctctgga accggcacct
     7141 ataagagcaa ttttaaattc cattttcata tcctagacat attcaagaaa tatgccatac
     7201 tccttctata ttttctatta tgctttttgt tatattttaa ttacatttta tctattgaaa
     7261 aatgttctta ataacaatgc tccctcttct ctagtatatt acaccctcgg aaaatcacaa
     7321 agtattaaat ttgcatattt caaagtacaa catttttgct tcaccctact tttttgatag
     7381 ttgcaacttt gggcttgaga tagagtgcct gctatagctt cttattatgg ctttccagtt
     7441 cacttatgga tgaaaaatca taaacttcag tttaatattc acttggatgt tatgtaataa
     7501 attataatcc aagtatccgt tccatatttc catggaatat tttctccctt aactcccgtg
     7561 aaaagcttaa tttatccatt agaatattaa acttattaac tgctccctgt attccctcat
     7621 aatggtcatc taccccgaaa ataaactttt ccttagccat attccagtat gttagagtat
     7681 caaaaaaaca catttccatt tcatataaaa tattaattgc tccactcaag tcaaaataaa
     7741 cattaggatt ggctcttgca accatgcatg cttcattgta ccagggaacg cctatatgtg
     7801 ctccgattat gtttaaattt ggaaaggctc ttgcgattcc atccaaaaat attggccgca
     7861 tcctttcaca tgcagtgtca aagtctccat ttccggatcc ccgtcccatt attccggtgt
     7921 ggaaaagtat tggcatcttg tacctggccg ccttttcata aacaaaatag tattccttat
     7981 cactataatt cttcttagga tttatgacct ttagaccttt aaatccttca tcatgaagat
     8041 gatctaccag acttggaggg tctataccaa gacgcacata tccaaaccct acaatcatgt
     8101 caggataaag attgaaggtc tgctttactg catcatttcc aggctgatta aattgttctc
     8161 cacaagcaga taaacaaact ttataaatac cgacatcctt tgacctttta atcaaatctt
     8221 caagagtatg tgtctttgag taataatgtt gatgtgcatc tattattttc ataattaatt
     8281 gttcctttcg ctgttaaatt aatatcatta ctactatttt aacaaagcgt caagaagttt
     8341 cccgcttcta caagcgggag ctgaattggc gcaaattata atagcaacct gttcagtgga
     8401 ggattacaag accctcactg aatctccgaa cgaagcaagt ttactacaat acaaaaatca
     8461 cggttcaaca ttcattttgt tattattgtg agctaaagct cttctcatat aatcatccag
     8521 aagctctatt tcaaattcct tagtaatatc tgatattgta aactcatctg tttcgctaga
     8581 aagcattact gagccaaggt atccaggatc tgtcagcttt actttaggcc catattttgc
     8641 ttttattctc ttaagttttt cccaattata atctaaatgt acaacacagt aatctaaatt
     8701 aaccatcgct gtaacaaaat catggtaatt tgtacttgaa gctattatct caccgaccgg
     8761 agaaataatt gtattttgaa gtcctgcaac acaacctgca aagtaggaac ggcatgaata
     8821 agcccaataa ttctgcatta aacctccatg atacattgat gagaaaagaa taatgtccgg
     8881 ttttgcttta acatatctta atcttagttc atcaaaatta agatcaaaac atatagcaca
     8941 agcaacacgt ccaaaatcgc aatcaataat gggagcatcc tttccacata atatcccgct
     9001 ctctgttgtt tcttcaatca ctacatagtt tttgttatag attccaacta ctttgccagt
     9061 acgatcaata atttgtgtag aatttctcaa tgtaccgtct cctgcttctc ttattgcgga
     9121 ataagctata tagcagttat tatttagtgc tatgttagaa aaaaactcca acatttgatt
     9181 tcctctgttt ttatagtatt ctgtcttctc cttttccagc tttggataat ttaaaggcct
     9241 atcacaccct tcaggtaaaa caattatatc cggcttatca ggcaagacct gatccagttc
     9301 gtgtttccaa taacttatta ctttttctac tgtctgagaa tagtcaatat caatatccga
     9361 taaaactggg cgtgcgccaa ttgaacttac ttttgtatat ctagccatat tatggttatc
     9421 ctcctgctta gaggccttct cctcttgaat tatctctttg tcattaccaa cttcccctta
     9481 tatttccttc tctatctgca atcatacttc cctttgtagc tattaattat ataaaacttt
     9541 caataagttt taatgaccag ctggttttgc acaatcgtta atataatcta taatcctaat
     9601 atttttgcca tatttccata gaaaatcttt tctcttaatt cctctgaaaa ctctagttta
     9661 tcaaataatg cattaaattt atcaattgca cccggtattc tctcataatg atcatccact
     9721 ccaaaaacaa acctttcttt tgcactttcc cagtgtgaca atgtcttaaa aaaccatggc
     9781 tcaaattcgt ataaaaggtt aatagcgccg cttaaatcat acaaaacatt tttccggtta
     9841 aatgccgcca ctgcacatgc ttcgtcatac cagggtacac caagatgcgc tccaattata
     9901 tttaattttg ggaatgctct tgcaatacta tcaaggtaaa ttggccgcat tctttcacag
     9961 gcaacatcat gttttatatc tgcaggattg cttattttca taccggtatg aaaaagtata
    10021 ggcattttta attcttcagc tcttttatat ataggataat attctttatc actataattt
    10081 ttcctcggac tgatagtctt caatcctcta aacccctgtt catatagttc atctacaagg
    10141 tgccatgtat caattccaag acgtataaac ccaaacccta taattgtatt gggatacttc
    10201 tcaaaagctt ttttaacatc ttcgttatac ttatcacatg aataatctcc caacccgctc
    10261 aagcatattt tagttatgcc tgctctttcc gatctttcca ccagttcctc gatagtttgc
    10321 ggttccggat aataatgttg atgtgcgtca atgatttgca taaatcattc ctcctatttt
    10381 tttaacgaag tttattcaac tacaatagct atagaattct taatttttaa cttgattgaa
    10441 attttgttta ccatattttc atcttttttc cactcaatct cactttcttt attattttct
    10501 ggatctacac cgtaaatttt attaacggta aaatcatctt ctaaaagaag ttcaattccg
    10561 atatcattat aacagtatcc atgcgtttta tttatcaaag ttacaacata cccaccagat
    10621 gggattttga atatgttacc tttataatta tcatctgaga tattcaaaca atgtgcttta
    10681 agataccatg aacgcttctt tagcagttct aatattttac cgtatccctt tagtaaggca
    10741 agcctttctt catcatcggc aggaagcatt tttgctgttt ccttagtacc ttttttatca
    10801 tcaggtttcc attcaacatg catctgtgag ccgcaaataa ttgcattttt aagcgcctct
    10861 tcaagctctg ttattttttc agttcttctt agcaacaata ttataggctt atttaatgcg
    10921 taatattgaa ctgttcccag ccattcccaa ctgcattcag ccattaagcc atcaatatat
    10981 ctcattactt caaggctcgt tggcccattg ccccatatgg atttacctct atttttaatt
    11041 ttttcataga ttaacttact aattttttct atagcaaaac caatcatata cgaaggcctg
    11101 tcgtttacag ccgtaatacc atcatcatga ttcatatcaa aatgcctgaa atgcaattca
    11161 tcaaaaaaca aaccatcgca ctccggaaag tagtttagta tcttctctgt ctggtaaatg
    11221 atattttgac cccagctcaa acccggatca ggattcatgg ggttattcag ataataattt
    11281 ttgaagcatg gaagtccatc aagtccaatt gcttcagaat ccttatactt tactttctca
    11341 tatttattta atataactgc atttatatat ataaatgcac ctactccata tttatgcaac
    11401 atttcaatat aatcgtttac atcttttctt gtaattttct tattatttga acgcttcatc
    11461 atacaattat ctatttccca atattctgca tcaaccaacc ttttcaaatt tagttttgat
    11521 atatcataag tatgtgaaat atcattccat tcttcttcct cgggagcata taacccgtaa
    11581 taagggaaaa tatttgcatg aatttcctgc catttaagac cttgctgcaa agcccaattt
    11641 ttaatatttt cttccggctg caaagtggaa cataacatgg taccttcagt ttcataaata
    11701 gaattgttgt ttacattaaa atattcagga tacaaatcat atatggcctt taaagagcat
    11761 ctccaatctc cttcatgaat ggaaaaaatc atcgatgtct taggtgaatc atattttgac
    11821 agccttaagt tacaatattt aaccacaaat gacaaaggag cattttctct gatataataa
    11881 tattgtacct gaggtttata tatatcaaca ggttggatta cagtaattcc atagttttca
    11941 tttttattat aaaaacctgc taaaggtaat gatatactat cacctaattc acctctgaat
    12001 tgatcccctc catatatgag cagcttatga tctatttctt ccggaagtaa atccgcattg
    12061 ctataaggtg caaaaccagt tatgtttgct ttaaaaaatg gaaggacata gactacttca
    12121 acttcagaca ttttaggttt agaagttatt ggagtacaat ttaatctgat atttccatcc
    12181 tccattttta attcaatatt taattctgta tcacatttac aggacgccaa atttatatat
    12241 aaaacattac tcttatcact tttgtttatt gagtaacttt tcattttcac atcatcagga
    12301 gtaaaacata attgcccggc agttttgtaa tgaagtgcaa tgtatccttt ttctccatcc
    12361 agtatatcaa tatttttttc tttaagtaga aattccacta tgttgcctgt ttctctgtca
    12421 atttttatat taaggcaatc attatcaaca ttaatataac tatcatattc gataatgctc
    12481 aggtattgat tttcatttaa attcatgcta ttataacccc tttttaagtt gctgttcttc
    12541 ataatctcct ctcaattttt gagtaaatga atgtttttat gaaatctccc caggtaaaga
    12601 aataaaatcc ttgcctgggg ggatttttgt gttaaattgt tattaattca aattcaatat
    12661 tgatttttac tgtaaactct gagttattac aataaaatca actttctatt tcatgaactt
    12721 gtctgcgagc actttccatc ttgattgcca atactcatat gacttctgaa cgcctgcgct
    12781 cttgaaatct gccaaaacat ttgcctttac agtttcgaaa tccttcggag ctgccataac
    12841 aagctttgga acagagttaa tagcgatttc gaggagagct gagtcaattc ttgctatatc
    12901 atcgggagct gtaccgaaac cttttaagat agctgtgtta cggttgcttc catctttaac
    12961 cccgcgagca taaagaacct cgttcgggta ggaatagtta gccttgtctc tatcactgta
    13021 aaatttaata tagtccttgc cattctcgtt gagacctgcg gtaaaatatg atttcgtcct
    13081 atctaaggca gtgatggaac catcagcaca cttaactccg cgtgctacac ctacaataga
    13141 gtagagagta taattgtcaa cacctgtagc tgcccattca tcgccgccag catttataag
    13201 ggccatggtt ttttccgtca agcatgcttc ccctttatca ttataatccc agtgttcgcc
    13261 tttaatacca ctgtagataa gccgggcggc ttccgggcta ttcaagtagt cgaggagctt
    13321 tatagcagca attttatcct tgcaggtgga agctatgcaa atgcagttac cgccgatatc
    13381 aaaaatgttt tcagtcatcc gtgcaccttt caaaggtata atttcccaac cttttgtgct
    13441 cattgcataa taatgattga gaatctgacc attctccatc ttcgtgctga aatcagctga
    13501 agtctgtata aagctgtcag gatcaagaat acctctgttg tatgcatcat tcatatattt
    13561 agccgccagc cagtatgggc ctttttcatc caggaacata taggataaag aattatcttc
    13621 aatttttaag gcaatatatg gattataggt actgacgcct acaacggaaa gtaatggacc
    13681 gtagaatgga taaagctggt tctcaacatt ataaaggctg aatgcataga tgggcttacc
    13741 atcagcagtt ttgggatgga gctttacaat ctgttcaagt gcatccacaa agtcaccaaa
    13801 gtcattcagc tcaggagcgc cgatttcttt ataataatcc caaattgtgt aggtgccaag
    13861 atttgtagca cgtgggttgc ctcctgccgg accaatggaa tttattatac cataaatact
    13921 tccatcgccg ttgtcgtatt tgtccttaag aattgtccac atgatgtcat tttcaggttt
    13981 cttaaggtta gtacaatatt tgtctatgag atcattaagt tttaatacaa gaccaccaga
    14041 agcaaggatc ttataattct ctgcatgata gcaagcgagc aaatcagtag tatcaccacc
    14101 tgcgataagc aagtcaaact ttgtcttgtc aacgtaagtg cttgtaagct ttacaccgga
    14161 ctgttttgtg aattctttat aaactggggt attatagaac ttttcgtcat cttccgcaat
    14221 ggtggtaatg atgttaagtt ctacaggttc tttttccttt acctcagggg cagcttttgt
    14281 agttgtatct gcttgattgg atgaattctt gccggatgaa caagccacca ttgataaaag
    14341 cataagagct gtgagaatta ctgccaatat tttcttcatt aaattatgcc tcctttaaaa
    14401 tatattgata tattctatat agaaagtctt cttctccaat tgcatgctca ttgggttaaa
    14461 agcttctata aatagacaaa taatgattta cccttttacg gcacctatca tgataccctt
    14521 tacaaaataa cgctgcataa aaggatatac taataaaata ggtatagtaa taaccgcagt
    14581 gacagtcata cgaatagcat caggggtcag ggagacatct acctttacac cgctttcaat
    14641 accttccctg atgcgatcgg ataaacgctg tgcttcctgt aaatattcgt ataagataag
    14701 ctgtaatggc tttaaattat tacgggtcat ataaatcatt gcatcaaacc atgaattcca
    14761 ttgtcctacc atggtaaata ctacaattgt cgccaggatg ggttttgaaa gtggcattat
    14821 tatcctaaac caacacataa gtatgcctgc accttcaatt ttggctgact cctcaagtga
    14881 ctggggaagc tgctcaataa aagtctttat taaaatcaca taatacgcag aaacagcacc
    14941 aggtaatata tacactaaaa agttgttacg caagccgaga tatcgcatta caagatatgt
    15001 gggaataaga cctccaccca catacatcgt taaaataaca aaacggtaaa tgaactttct
    15061 aaaatacatc ttttgctgcg tcatcaagta tgcaaaaaag cctgagcaaa aaagggttac
    15121 agaagaacct aatatagttc tggtaacaga aaccaatgct gcttgcggaa tttccttaag
    15181 ttttatgaca tttatatagt tgttaaatgt aaaaccgcgg acccaaaggg ttattccctt
    15241 cgtagcaagt accggatcac tgagcgaata acaaagcata tagtaaaatg gataaacaca
    15301 aaaaattgaa aacataatga caatcaaaat atttgatata tcgaacaatt tggaaccaaa
    15361 acttttttta ccaatcatat tatcgactct cctcttactt tcctcgccag tttattgact
    15421 gtgaaaagaa gtacaatact gacaagggat tttgcgatgc ctatagccgt tgcaaacgaa
    15481 tagtcgtggg tcattaggcc tatacggtat atatacagat ccaaagcctc aatttttgaa
    15541 gcattaagag gatttttaaa attcaagtat ttatccagac caatagataa tagattcccc
    15601 actgatagaa gaagcagtac aatataagtt tccatgagat taggcactgt gatatgccaa
    15661 atacagcgga agcgtcctgc accgtcaatt atcgccgctt catagagttc agcatcaata
    15721 ccggcaatag cggcaagata aataattgta ccccatccca ggcccttcca aatgtcaagg
    15781 gttgtcatga atgtccagac gtaatttgaa tttgaaagga cagtgtttgg tttatcggta
    15841 atatttaaaa cctgcatcag gttattatac ataccgttag tggagaaaat agcaaagcac
    15901 agagaatatg caattaccca actgataaaa tgaggaagcg ttacaaagct ttgaatgaat
    15961 tttttaaatc ttaaattacg aagttcgtta aggagtattg caaatataac tggtagtacc
    16021 gaagttgcca gcgacataaa actcattgca agggtgtttt taagtacacg gatcatatcc
    16081 ttattgtagc tgaatatata cttaaagtat tttaatccca catattctgc ctgtgacaaa
    16141 gatagtcccg gcttataatc aaaaaatgca aatgaccacc caaaaagcgg caggtagcaa
    16201 aatacgaaca cgaaaattac aaacggcaga gccaaaagag ttaactttat tccttcaatg
    16261 cttattttcc ttttccgaag tgttgaatcg aggccaattt tcaatattta caactccttt
    16321 gtaagtcata ttgaattatt aatccttata ataactacat tgtataatga gttaaaaaat
    16381 gtgtcaattt aacctttctg ttatttatga tacttatcaa cacttatgaa gtttatatcg
    16441 tacatttgtg tcacagaatt gtactattta ttcaaaattg ttcaattcta tatataacgc
    16501 aaaaatgatt cgaaaaggta caaattgaat ttttacatac cttcatttag gtgcaagtat
    16561 gcacctttat tcactaaggt aacataagca cataaaattt atataatgta ttttcttttg
    16621 tgcattatct atagtttata tctatttcat gccggttgct catccaacgg cagtgttata
    16681 gtaacccgca gcccgcatac atggcctcgc tctacatgca caccgtagcc tgcaccatac
    16741 tgaagtctca acctgcggtt cgtattcata agccctatac cattggaatc gttgccattg
    16801 cgcatccgct ggttgagttg agctatatct tcatcactca taccgcttcc attgtcatct
    16861 acgcaaataa tcatcgcatt attttgctta aatgcgctaa gaatgatatt tccttgccct
    16921 aaactcttct caaatccatg ggttatcgca ttttctacca gtggttgtat aataagacgc
    16981 agtatcttcg tatccagcag actctcatct acccgctttt catatataat accatcatca
    17041 aggcgtgtgt tcatgatgtt tatgtataaa tctatggttt caagctcctc acaaagtgtg
    17101 caaaccggtg tactgctttt gaacataggt gaaatgatac cgcacagcat tgtgatacaa
    17161 tcggcagcat tacgattacc ttcaataagg cacatccact ttatggtgct gagtgtattg
    17221 aataaaaagt gcgggtttat ctgattccgc agagctttta gttctccttc acgtttttcc
    17281 tgctctacta tctcattttg tttcttaagc ttttgcatgt tctttaacat gtcattgtag
    17341 ctcatgataa gcattgacaa ctcatcatac cctgtagcag gtaaaagttc atcacaggat
    17401 ccttgaccag tgcggcgcat actgcgtttg agcgtaataa gcggtttgaa tgccttaccg
    17461 agccataggt aaaataaaat gcatgccagg atgaagcccg gtataattat acacaaaagc
    17521 gtctgtgata tctccgttaa ttcactgaaa tactcctgta aaagtatatc gcttgtaata
    17581 atcagctttg catccggcat tgtctcccat atcatctggc tttctgtacc tgtgtcggta
    17641 tactgccctt tattgcttcc ggtaataata t
//
LOCUS       DAOSDD010000076        17551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1313_length_17551_cov_5.141861, whole genome shotgun sequence.
ACCESSION   DAOSDD010000076 DAOSDD010000000
VERSION     DAOSDD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17551
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1313_length_17551_cov_5.141861"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK733_08515"
     CDS             <1..248
                     /locus_tag="PK733_08515"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10622.1"
                     /translation="SWMMDYQLRQFLSENSGIVRFQHRYLLYPRFTTGKAVFTFVFNK
                     PFIEKLEDLPEDTSLLHAIKYHYLNGKYIYEQGGVFF"
     gene            complement(688..852)
                     /locus_tag="PK733_08520"
     CDS             complement(688..852)
                     /locus_tag="PK733_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10623.1"
                     /translation="MSKRKLEINKKHSSYEELEAFYRGCNDTKLRTRILAVLQTWDGK
                     PSTEVAKIFI"
     gene            998..2302
                     /locus_tag="PK733_08525"
     CDS             998..2302
                     /locus_tag="PK733_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ10624.1"
                     /translation="MINKIKYMPGELSRVLAEDGISVVITGDSLAYNRYDFDHEPRVN
                     AYDCYPGMPSWSFMLRDAIHQNDKWYVFGDEINYQGRSFIKENALLGDDVPITGNAPE
                     SYDHEYESFHDAKYSCLNHGKTASLFIDKSDRNISFLYSHSNEDTNKAVLYMQKRPDE
                     FSCSFDIYLDGKLALSGINIDGKDGQFQGWEPFEVEIPVKGDGKPHEITFRNIRNVNT
                     INNIKKINKINNIKHGQNTNRIEVHEGRTANLESRINIAGIGTKRARIHLTGNGGKTT
                     QYFLDNMEERIMQYEPDVLIFIIGANDRAYLSVEEFNANLRRIMNIIKGKRPACEILL
                     ITPPSSANMEKPAEDNLPVYITDKSSQSYIEAMYRISREYGCMFLNLIELLKNVPIKE
                     WRYDNVHFTKYGNTLVAKAVLNMLLGNGGYYRKELVNAEVWF"
     gene            2343..2666
                     /locus_tag="PK733_08530"
     CDS             2343..2666
                     /locus_tag="PK733_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10625.1"
                     /translation="MIPVFLQGCGADIKPYITADNDRFKSCNYEELEEAVKSLAKEIQ
                     GLIENSEEKDEIVVIGEKLISILKQKAVRLSYIQKYGILRNGKLLLMIRKNRYTGKLL
                     RKRYF"
     gene            2892..3926
                     /locus_tag="PK733_08535"
     CDS             2892..3926
                     /locus_tag="PK733_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOJ10626.1"
                     /translation="MECLWVLEPDAAYGGLNNKHDKGYKHLLSTKRVFIQLLKSFVDQ
                     DWVNKIDEESAEKIDKSFILQDFSGKEADLVYKIKINGREVFFYILIELQSTVDYQMP
                     YRLLQYMLEIWRAILRDTEVKESERKDFKLPVIVPCVLYNGQYNWRVCTNFKETLVAN
                     ELFDDYILDFNYILFDVARYSEEDLLKLGNLIGAVFYIDQKPQYEEMIKRLNNLVEHL
                     KMLSDEDFKLFRVWLKNVAARGMTKEETGEIEKIIDENEEAGKMVYAVEIAIRNKIEE
                     MKKQGLEEGFKEGIEKGIEKGFEKGINEGIVRGKIEVVKNLLDMGMDIEQISIATGFP
                     KENIQEFIVT"
     gene            4042..5160
                     /locus_tag="PK733_08540"
     CDS             4042..5160
                     /locus_tag="PK733_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10627.1"
                     /translation="MLKLEMLQKEILKRELPPLLTMNDGRLCTAELWRERRTEILDIL
                     QKNIYGYTPKSLKKTSGEVVEQSRKNAFAGKVNQQHISIKFDTPNGEFSFPLYLFLPR
                     SNSCAPLFLHIAFRPDIPDIYCPIEEITDNGFALAFIYYKDIVNDNLHGDYSDGLGKL
                     YIDKRQRHPDEWGKIGMWAYAASRALDYLLTRDEVDHKHIAVVGHSRLGKTALWCAAQ
                     DERFFMGISNNSGIGGAAIAKHSTGERVSDFIRAGSWDWFCETFKGYSGYEDINMPYD
                     QHFVLAAIAPRHVCVGSAELDKGADPKSEFLSCVAASQVYKLLGYHGIVTPDEYPAAG
                     TNLHEGEIGYHIRTGLHYFSRYDWNQYMKFMKKKIDSE"
     gene            complement(5369..8887)
                     /gene="nifJ"
                     /locus_tag="PK733_08545"
     CDS             complement(5369..8887)
                     /gene="nifJ"
                     /locus_tag="PK733_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOJ10628.1"
                     /translation="MGKKMKTMDGNTAAAHVAYAFTDVAAIYPITPSSTMAELVDEWS
                     ANGRKNIFGQTVKLSEMQSEAGAAGAMHGALASGALSTTFTASQGLLLMIPNMYKIAG
                     ELLPGVFHVTARALATHALSIFGDHTDVMATRQTGVAMLASGSVQEVMDLAGIAHLAA
                     IKARIPFLHFFDGFRTSHELQKIEVMEYEDLAKLIDYEAIERFRKNALNPEHPVTRGT
                     AQNPDIYFQAREACNKFYDAVPDIVESYMQEITKITGREYHPFVYYGAEDAENVIVAI
                     GSVNEAIEETVDYLMKKGEKVGVLKVHLFRPFSEKYFFNVLPKTVKRIAVLDRTKEPG
                     AVGEPLYQDVRTMFYNKESAPLIVGGRYGLSSKNTTPAQIKAVFDNLKADQPKDGFTI
                     GIVDDVTHLSLPVGERIKTTPEGTISCKFWGFGSDGTVGANKNAIKIIGDNTDMYAQA
                     YFAYDSKKSGGVTVSHLRFGKKLIKSTYLIEEADFIACHKESYVHQFDILEGLKDGGT
                     FLLNCQWSPDELKEKLPFKMRKYLKEHNIDFYIVNAFDIAAKIGLGGRINMLMQTVFF
                     KLANIIPVEDAVKYLKDAIQKTYGKKGEKVVKMNWDAVDHALDAAVKVDVSTLISDGE
                     QEQPAAEGFKPDFVKNILEVINRQQGDTLPVSAFTGVEDGTFPQGTAAWEKRGVAISV
                     PEWQISNCIQCNQCSLVCPHAVIRPYLLDDDEKAKAPDTFVTKSALGKELKGLHYRMQ
                     VSVMDCTGCGVCAQACPAKEKALIMKPFEEQVDVQSANFEFAANNVKTKDNLIDKFTL
                     KGSQFSKPLLEYSGACAGCGETPYAKLVTQLFGDRMFIANATGCSSIWGGSAPAMPYT
                     VNDKGCGPAWANSLFEDNAEYGFGMYLGISQIRSKLAELANELVNANIDSDLNTALNE
                     WLENMNKGEESKAAVEKLLPLLEKNSGNSIAAEILSKKEFLIKKSQWIFGGDGWAYDI
                     GFGGLDHVLASGEDVNVLVFDTEVYSNTGGQSSKSTPTAAVAKFAASGKKIRKKDLGR
                     MAMTYGYVYVAQISLAANMNQAIKAIKEAEAYPGPSLIIAYAPCINHGIKGGMTNSGL
                     QQKKAVETGYWHLYRYDPQLKDEGQNPFSLDSKEPTGSFRDFISSEVRYTSLKTTFPE
                     IAEEYYAKAEQDAKERYETYKKMAEG"
     gene            complement(9527..10072)
                     /locus_tag="PK733_08550"
     CDS             complement(9527..10072)
                     /locus_tag="PK733_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ10629.1"
                     /translation="MEYIIPGIILLIVLIGIFSYNGLVGKRNRVRNSWSQIDVQLKRR
                     FDLIPNLVETVKGYASHEKGTLEAVVSARTKFLSAKTPEDMMKANGELSQVLGRLFAV
                     AEAYPDLKANTNFMHLQEELSKTEDKIGYARQFYNDVVLDYNNAVQMFPTVIIASMFG
                     FREEPFFKAEEEERNNPKVSF"
     gene            complement(10164..10835)
                     /locus_tag="PK733_08555"
                     /pseudo
     CDS             complement(10164..10835)
                     /locus_tag="PK733_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(11388..13310)
                     /locus_tag="PK733_08560"
     CDS             complement(11388..13310)
                     /locus_tag="PK733_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOJ10630.1"
                     /translation="MDQRKKYILRVISSISFILSIFLILCFYAEPAIAENNRKYFISK
                     YNIYINISTDGSANIEEHITYSFSGNFNGVTFDIDLSGTKGIKDEQVFILKDGKQVEV
                     VRNNSESPGTYLYNLNSSMAKFKVYEPSRNEEKTFIYKYTLVDAVVKYNDIAEFNRKM
                     IGTEWQAPLNNIHIKTILPQGASKGDIRVFGHGPLTGESDIIDEKTVEFKVPAVAPGT
                     FVETRVLFPVKLVPDSKNIVSKGALQDILQSEGKLAEEANLKRENAREQLRKQQEKEK
                     ALNVIGTVIFYILLPIWLLLVIYIYLKYDREFKRRFFGKYYRELPGEYTPAEMSLLMT
                     MGKVQSRDVMATLMDLVRKKNLLLEQVTVNKKGLFKNKEVKDYKISLNTEKPLTSLKS
                     HEAFLIKWFINKIGDGNSVLLDDIKDFARDKDNALDFKADYDLWCEKAENEAKLNKFF
                     DDSAKKGKIIGILSSIVIIIIGILMPGIFKNTVGITLLPLGIIMLLFSARIKRRSTYG
                     NEQYAMWKAFKRFLKDFSRLEQAEVPSIILWEHYLVYAISLGVAREVIKQLPLVFKED
                     DLGNPQLTYLYGMSYGHFSNLDRVFNNTIKTVESSVMTATTIANSRNSSSTGSGGGFS
                     GGSSGGGGGGGGGGAF"
     gene            13647..14096
                     /locus_tag="PK733_08565"
     CDS             13647..14096
                     /locus_tag="PK733_08565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ10631.1"
                     /translation="MAVLKFKADAYGENPTKTVVEARNFKITIDEPENLGGTDQGANP
                     VEYILAALAGCLNVVGHVVAKEMGFELGRMHIELEGDLDPARFMGRTKAERAGYKEIR
                     VKISPETNIDHNSLEKWLETIEDRCPVSDTIQNITPVKIELNTTAGY"
     gene            14409..15134
                     /locus_tag="PK733_08570"
     CDS             14409..15134
                     /locus_tag="PK733_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOJ10632.1"
                     /translation="MNYSIMAYIISAGAVALAEMADKTQLMAMAFASKYKVSKVSLGV
                     FLSIVFSQGMAVAMGNYMGQHQGLNVWIQAIASLSFIFFGLWTMRGEKSEDMKVQNSK
                     FGVVITVAIAFFIAELGDKTQLATIAFAIKYPSNPISVLLGTITGMVVANALGIFVGV
                     VLCKRIPEEKLKFLSAGAFILFGFIGVYQVMADKLNFTTGTIAVIMILIALITGTAMY
                     FIFKNREIPEKRGFNQYCRIKGR"
     gene            15453..16673
                     /locus_tag="PK733_08575"
     CDS             15453..16673
                     /locus_tag="PK733_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10633.1"
                     /translation="MGKNVSLLLVGIGGYGNNYVKGILNNMGDMAKDRFTIKGIVDPN
                     PSGCQYINELKDMGIPFYNSLDEFYAVDSADLAVISSPIQYHAPQSCCALEHGSNVLC
                     EKPICATIQDALKMIDARDKSGKFLAVGYQWSYEEAMLDLKRDIINGKLGRPKRLKTI
                     VLWPRDKKYYTRSSWAGRKQDDRGNWILDSVANNATAHYLHNMFFVLGNKLDTSAYPA
                     SLRAELYRANEIENFDTSVIQAYTDDGVEILFYATHAVKETKGPIFQYEFENGIVNFG
                     GETGEKRIISATFNNGTTKIYGEPYSTEIRKLWVCIDAAAGIGDEITCKAETAIPQIL
                     CINGAQESMPEIVKFPDTLIKREGEPELTWVEGLSESLIQCYNNNTVPTAMGISWAKT
                     GKEINLKGYCSITL"
     gene            16869..>17551
                     /locus_tag="PK733_08580"
     CDS             16869..>17551
                     /locus_tag="PK733_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ10634.1"
                     /translation="MFAYWAMMLSISGMYAMYIAQIGFSKKEISIAVTIFTFAALIGQ
                     NVFGYLADRFKCIKKILLVAISVGIIASIALNFSKQSWFISILIFLWGFFLYGTVPLS
                     EAWYIGILKANGDQREFGKIRGIGSIGYGISGIILGLLLQNIGWNLYSWYILISSCAV
                     LLVITLMSESKEIALYKAGGGDGCGDTNNASFKEAFEQIITIKSLRTIIIIVFIYSFV
                     LKGIYSYLGV"
BASE COUNT         5808 a         2959 c         3259 g         5525 t
ORIGIN      
        1 cttcatggat gatggattac cagcttagac agtttctatc ggagaattca ggcatcgtac
       61 gatttcaaca tagatatcta ttatatcctc gatttacaac aggcaaggca gtatttacat
      121 ttgtatttaa taagccattt attgagaaac ttgaagatct gcctgaggat acaagtctcc
      181 tgcatgctat taaatatcac tatctgaatg gtaaatatat ctatgagcaa ggtggtgtat
      241 ttttctgagc aaattctaca attgaatatt gaagtattaa atatatagta ttgattttag
      301 tgcaataact tagattttgc tgcaaaaggc aaataaaggt taaggtatag aataggattt
      361 aaaaaggctt ttttgaaaaa catgaaagga atgattataa caatgaaaaa gattgatatg
      421 cacatccatt taagtgaagg tataatacag aaccatattg gacctaggac attcacagcc
      481 cttttgggca gaaattagtt ccgatgtaaa ttaagatagt agaaaaggtt atcaagtagg
      541 taaagttatt cccggaaggc ttgttgagct tatgcgtaaa tatccaaatt tatatgggga
      601 tctttctgcc agaagtggat ttaatgctat ttcaagagac cccgaattcg ttataccgat
      661 gcacccattt acggacatta gtatctgcta tatgaatatc ttagcaactt cggtacttgg
      721 tttaccatcc caagtctgta atacagcaag tattcttgtt ctaagttttg tatcattgca
      781 gcccctgtaa aaagcctcaa gttcttcata ggaggagtgc tttttattta tttcaagttt
      841 ccttttactc ataatattag agcctttcta taaaagtgtt ataatattag tataacttat
      901 agcagtatag gtattataca ggtaatataa tttaagtaca tttatgaaag cctaaactat
      961 ataggtaaaa ttctgaaata aggaggcata aaaaataatg attaataaaa ttaaatatat
     1021 gcctggagaa ttatccaggg ttttagctga agacggcata agtgttgtta ttactggaga
     1081 cagccttgct tataatagat acgattttga ccatgaaccc agggttaatg cttatgattg
     1141 ctacccggga atgccttcgt ggagctttat gcttcgtgat gccatacatc agaatgataa
     1201 gtggtatgtt tttggagatg agattaatta ccagggacgt agttttataa aagagaatgc
     1261 gctgttaggt gatgatgttc cgataacagg caatgcacca gagtcctacg accatgaata
     1321 tgaatcattc catgatgcta agtattcctg ccttaaccat ggaaagacag cttcactttt
     1381 cattgataaa tctgaccgga atatctcatt tctgtacagt cattcaaatg aagatactaa
     1441 taaagctgtg ttgtatatgc aaaaaaggcc ggatgagttc tcctgcagtt ttgatatata
     1501 tttagatgga aaacttgctt tatcaggaat aaacatagat ggaaaagacg gtcaattcca
     1561 gggatgggaa ccatttgagg tggaaatacc cgtaaaagga gatgggaaac cccatgaaat
     1621 tacttttcga aacattcgca atgttaacac tattaataac atcaaaaaaa ttaacaaaat
     1681 taacaacata aaacatggtc agaatacaaa tagaatagaa gttcatgaag gaagaacagc
     1741 taatttagaa agcaggataa atatagcggg tataggaaca aaaagagcaa ggatacatct
     1801 tacaggcaat ggaggcaaaa caacccaata ttttcttgat aacatggaag aacgtattat
     1861 gcagtatgaa cctgatgtac ttatttttat aataggggca aacgataggg catatctcag
     1921 tgttgaggaa tttaatgcaa atctcagaag aattatgaat atcattaaag gcaaaagacc
     1981 agcatgtgag attttgctga ttacacctcc gagtagcgcc aacatggaga aacctgcaga
     2041 ggataatttg ccggtttata ttacggataa atcatcacaa tcatacatag aagccatgta
     2101 taggataagc agagaatatg gctgcatgtt tcttaacctt atagagctgc tcaaaaatgt
     2161 gcctatcaag gaatggcgtt atgataatgt tcattttaca aagtatggaa acactcttgt
     2221 tgcaaaagct gtacttaata tgttattggg aaatggcggt tattacagaa aagagcttgt
     2281 aaatgcagaa gtctggtttt agcggatcac tttttaacct ttgccgctaa ttaaatccag
     2341 gaatgattcc ggtatttcta cagggctgtg gtgctgacat aaaaccgtat atcactgctg
     2401 ataatgatag atttaaaagc tgcaactatg aagaattaga agaagcagta aagtcccttg
     2461 ccaaggaaat acaaggtcta atcgaaaatt ctgaagaaaa agatgaaata gttgtaattg
     2521 gagaaaaatt aatttcaata ttaaaacaaa aagcagtgag gttaagttac atacagaaat
     2581 atgggatact gagaaatggg aagctattat taatgatccg gaagaaccgt tatacaggaa
     2641 agcttctgcg aaaaagatac ttttagatat gaaagatgat aaaattatct tccatattac
     2701 ataccatttt taaggctgga tgaaaaaaca agtatagttg ctttttcaat taaattgaat
     2761 acattaattt gtatgttaaa agatggatta aagtggaaac aattcaatat atttcttgac
     2821 aataaacata tgctcaggta aaatgttatt gaaaattacg ttgttatact ataaaatagg
     2881 ggtgaatatg tatggaatgt ttgtgggtat tagaacctga tgcagcatat ggaggattga
     2941 acaacaaaca tgataaaggg tataaacact tactttcgac gaaaagggtg tttatacagt
     3001 tattgaagag ttttgtagac caagactggg tgaataaaat tgatgaagaa agtgctgaaa
     3061 aaatagataa atctttcata cttcaggatt ttagcggaaa agaagctgat ttggtataca
     3121 aaataaagat taatggcaga gaagttttct tttacatttt aatagaattg caaagtactg
     3181 ttgactatca aatgccatac aggttactgc aatacatgct ggaaatatgg cgggctattt
     3241 taagggatac ggaagtaaaa gaatctgaga gaaaagattt taagcttcct gtaattgtgc
     3301 cgtgcgtatt atacaatgga cagtataatt ggagagtatg tacaaatttt aaggaaactc
     3361 ttgttgcaaa tgaattattt gacgattata tacttgattt taattatata ctttttgatg
     3421 tagcaagata tagtgaagag gatttactaa aacttggaaa tttaattggc gcagtatttt
     3481 acatagatca aaaaccgcaa tatgaggaaa tgataaaaag actaaataat ctggtagagc
     3541 atttgaaaat gcttagtgat gaggatttta aattattcag agtttggctt aagaatgttg
     3601 cagcccgtgg aatgacaaag gaagaaactg gtgaaataga aaaaataatt gatgaaaatg
     3661 aggaggctgg taaaatggtt tatgctgttg agatagcaat acgtaataag attgaagaaa
     3721 tgaaaaaaca aggcttggaa gagggattta aggaaggaat tgagaaagga attgagaaag
     3781 gatttgagaa gggaattaat gaaggcattg taagaggtaa aatagaagtg gttaaaaacc
     3841 tgcttgatat gggaatggat atagagcaaa taagcattgc caccgggttt ccaaaagaaa
     3901 atatacagga gtttattgta acctgattag ataattggaa gttaacggga taaagatatc
     3961 attgattaag gaaacgtaga gtgaaaataa aatttaatta ttatttatgt tgccattggt
     4021 agcagcggaa tggaggttac aatgttaaaa ctagaaatgc tgcaaaagga aattttaaag
     4081 cgtgagttgc cgcctctgct gacaatgaat gacggaagat tatgtactgc agagctgtgg
     4141 agagaacgca ggacggaaat attagatatt cttcagaaaa atatttatgg ttacacacca
     4201 aaatcactga aaaaaactag cggtgaagtt gttgaacaga gtcgtaagaa tgcttttgca
     4261 ggaaaagtga atcaacagca tataagtata aaatttgaca ctccaaatgg tgaattctca
     4321 ttcccactat atctcttttt acccagaagc aattcttgtg caccattgtt tttacatatt
     4381 gcatttcgac ctgatattcc tgacatatac tgccctatag aagagatcac cgacaatggt
     4441 tttgcgctag ccttcattta ttataaagat atagtaaatg acaatttaca tggtgattat
     4501 tctgatggac taggtaagtt atatatagac aaaagacaaa ggcatcctga tgaatgggga
     4561 aaaattggta tgtgggcata tgctgcaagc agggcactgg attatcttct gacccgtgat
     4621 gaggttgacc acaaacatat agctgttgtt ggacattcca gactgggcaa aacagcactt
     4681 tggtgcgctg ctcaggatga acgttttttc atgggaatat ctaacaactc aggaattgga
     4741 ggtgcagcta tcgcaaagca ttccaccggt gaacgtgttt cagattttat aagagcagga
     4801 agttgggact ggttttgcga aacatttaaa gggtattccg gttatgaaga tattaatatg
     4861 ccatatgacc agcactttgt gctggcagca atagctccaa ggcatgtgtg tgtaggaagt
     4921 gcagaacttg ataaaggcgc agatccaaaa tccgagtttt tatcttgtgt agcagcaagc
     4981 caggtgtata agcttttagg ttaccacgga attgtaactc cggatgagta tcctgcagca
     5041 ggcacaaacc tgcatgaggg agaaatcggt tatcatatcc gtactggtct tcattacttt
     5101 agcagatatg actggaacca atatatgaaa tttatgaaaa agaaaataga tagcgaatag
     5161 taagaaatag atatagatag cgaataataa gacatagata ataaataata aggtatatgg
     5221 gagtatcaga catattaaat aaattacctg cttattttaa attagcccag gttaaaatca
     5281 aatttaacct gggctaatat taattctact aattctacta attcaatatt gaaactaata
     5341 ttagttttaa tattagttag cttaaccctc agccttcagc cattttctta taagtttcat
     5401 acctttcctt tgcatcctgc tcagcttttg cataatactc ttcagctatt tcagggaagg
     5461 tggtttttaa gctggtataa cgcacttcac tagagataaa gtctctaaag gaacctgtag
     5521 gttcttttga atccagagag aaggggtttt ggccttcgtc tttaagctgt gggtcatacc
     5581 tgtaaaggtg ccagtaaccg gtttcaacag ctttcttctg ctgtagaccg ctgtttgtca
     5641 ttccgccttt tataccatgg tttatacatg gagcataggc aattattaat gatggtcctg
     5701 gataagcttc agcttcctta attgctttta tggcctggtt catgtttgct gcaagtgaaa
     5761 tctgggccac atatacataa ccataggtca ttgccattct gccaaggtct ttctttctta
     5821 tctttttacc ggatgcagca aattttgcta ctgcagctgt aggtgttgat ttggatgatt
     5881 gaccgccggt attggagtag acttctgtat caaataccaa tacatttaca tcttcgccgg
     5941 atgccaatac atggtcaaga cctccgaatc caatgtcata tgcccaaccg tcaccaccga
     6001 agatccactg agatttctta attaaaaatt ccttcttgct aagaatttca gcggcaatac
     6061 tattaccaga gttcttttca agcaaaggca gcaacttttc aacagcagct tttgactctt
     6121 cgcccttatt catattttcc agccattcat taagtgctgt gtttaaatca gagtcaatat
     6181 tggcatttac aagttcattg gcgagttctg caagtttgct gcggatctga gatataccca
     6241 gatacattcc aaagccatat tcagcattat cttcaaatag tgagttagcc catgcaggac
     6301 cgcaaccctt atcatttaca gtataaggca ttgccggagc ggaaccaccc caaattgaag
     6361 aacatccggt agcattggca ataaacattc tgtcgccgaa aagctgagta acaagttttg
     6421 cataaggagt ttcgccacat ccagcgcaag caccggagta ctcaagtaat ggttttgaga
     6481 actggctgcc tttaagagta aatttatcta ttaaattatc ttttgttttt acattattag
     6541 ctgcaaattc gaagtttgca ctctgaacat cgacctgttc ttcaaaaggt ttcataatta
     6601 aagctttttc tttagcggga caagcctggg cacaaacacc gcagcctgta caatccataa
     6661 cacttacctg catgcggtag tgcaaaccct tcaactcttt accaagagcc gattttgtaa
     6721 caaaggtatc gggtgctttt gccttttcat catcatccag caaataaggt cttataactg
     6781 catgtggaca taccagtgag cactggttgc actgtataca gttactaatc tgccattcag
     6841 gaacactaat agctacgccg cgtttttccc atgcagcagt accttgtggg aatgttccat
     6901 cttcaacccc tgtaaaggca cttaccggca gtgtatcacc ttgctgacgg ttgataactt
     6961 caagtatatt tttaacaaaa tcaggcttaa atccttcggc cgctggttgt tcttgttcac
     7021 catcggatat taaagttgaa acgtcaactt taactgctgc atccagagca tgatctactg
     7081 catcccagtt cattttaaca actttttcgc cttttttacc ataggtcttc tgaatagcat
     7141 ccttcaaata ttttactgca tcttcaacgg gtattatatt agccagcttg aagaatacgg
     7201 tttgcattaa catattgatt ctgccgccca gtccgatttt agcagctata tcaaaagcat
     7261 taacaatata gaaatcaatg ttatgctctt taaggtattt tctcatttta aaaggaagtt
     7321 tttccttcaa ctcatcaggt gaccattgac aattcaataa gaaagtaccg ccatctttca
     7381 gaccttcgag gatatcaaac tgatgtacat aagattcctt atgacatgct ataaaatcgg
     7441 cttcttcaat aagataagtg gattttataa gtttcttgcc aaaacgcaga tgagaaacag
     7501 ttactcctcc tgatttcttg gaatcataag caaagtaagc ctgagcatac atatcagtat
     7561 tatcaccgat tatcttaata gcatttttat ttgctccaac agtaccatca gacccaaaac
     7621 cccagaattt acagcttata gttccttcgg gagttgtctt tattctttca ccaacaggta
     7681 gtgacaggtg ggtcacatca tcaacaatac ctattgtgaa tccatcttta ggctgatctg
     7741 ctttaaggtt gtcaaatact gctttaatct gtgcaggagt agtattcttg gaacttagtc
     7801 catatcttcc accaacaata agaggagcac tttccttatt atagaacata gtgcgtacat
     7861 cctgatacag aggttcgcct actgcaccag gttcctttgt tctatcaagt actgcaattc
     7921 ttttaactgt ttttggaagt acattaaaga aatatttttc agagaaagga cggaaaaggt
     7981 gaactttaag tactccaacc ttttctccct ttttcataag ataatctact gtttcttcta
     8041 tggcttcatt aactgaaccg attgcaacaa tcacgttttc agcatcttca gcaccataat
     8101 atacgaaagg atgatattct cttcctgtga ttttggttat ctcctgcatg taggattcta
     8161 caatatcagg tacagcatca taaaatttat tgcaagcttc tcttgcctgg aagtatatat
     8221 cagggttctg agcagtaccc cttgttacag gatgctcagg atttaatgca ttcttcctga
     8281 atctctctat ggcttcataa tctatcagtt ttgccaggtc ttcatattcc ataacttcga
     8341 ttttctgcag ttcgtgagat gttctgaaac cgtcaaagaa atggaggaag ggaattcttg
     8401 cctttattgc ggccaggtgg gcaattccag cgagatccat aacttcctgg acacttcctg
     8461 atgcaagcat agctacacct gtttgacggg tagccataac atcagtatgg tctccaaaaa
     8521 tcgaaagtgc gtgagtagct aatgcacgag cagttacgtg aaatactcct ggaagaagct
     8581 ctccagctat tttatacata ttaggtatca taagtagcaa gccctgagag gctgtaaatg
     8641 tagtagataa tgcgcctgaa gctaatgctc catgcatggc acctgcagct cctgcctcag
     8701 attgcatttc agaaagtttt actgtctggc cgaaaatatt tttacgtcca ttggcactcc
     8761 attcgtcaac cagttctgcc atggttgatg atggggtgat aggataaata gctgctacat
     8821 cggtaaacgc gtatgccaca tgggcagctg cggtattacc atccatagtc ttcatctttt
     8881 ttcccatttg attttcccct ttcacaacat aatttaatta aataaaatta aataattttt
     8941 tattatataa cttaacttta tcccggaatc cggcgcattt tgcttccaac tgcagaaacc
     9001 tttcgagttt tatagtaaac tgtcgtataa tttccttatc ggaaccggtt aaagtttaaa
     9061 tagcaacata tataacacca agtatcacaa tatataatat cataataaaa gcacttgtac
     9121 caataataaa ctaattatat tttagaaaat ataattagtt gttcaaaagt gctaaaaaac
     9181 gcactttaca gcactaatac aataaatata atgtaatact gtatacatta ttacttatta
     9241 ttgtgccaat gatattatac ataataattt aaaaaatctc aaataaaatt agcaacacaa
     9301 taaaataaat tttattgatt tttcttgaat aacacttgat gaaattgagt tacaaggcag
     9361 tagaggccta tatcaagcat agggcctcca caatcttctg aaactctgca caactaaagt
     9421 cggcatgttc taatgtactt tttaacactc tgaatctaaa agtatgggga gcacaatcct
     9481 gtttatctgg aattcattgt acctgacata gcatgtatag atctcattaa aaactaacct
     9541 ttggattatt tctttcctct tcttctgctt taaagaaagg ttcttccctg aacccgaaca
     9601 tagatgcaat tataactgtt ggaaacattt gtactgcatt attataatcc agcacaacat
     9661 cattataaaa ttgacgggca tagcctatct tgtcttcagt ttttgagagc tcttcctgta
     9721 aatgcataaa atttgtgtta gcctttaaat cagggtaagc ctcagctact gcgaagagtc
     9781 tgcctagaac ctgagataac tcaccatttg ctttcatcat atcttccgga gtttttgctg
     9841 aaaggaattt tgttctagca gaaacgacag cctcaagagt tcccttttca tgagaagcgt
     9901 atccttttac tgtttccaca agatttggaa taaggtcaaa ccttctttta agctgtacat
     9961 caatttgact ccatgaattt cttaccctgt ttcgctttcc tacaagacca ttgtaggaaa
    10021 aaatgcctat aagaacaatt aacaggatga ttccgggaat aatatattcc atataatagc
    10081 ctccaatcag aatattatat ctaataatat ttatatatta ttactgcatt attaatacgt
    10141 caactattat ttaaattact tacttagccc accacacaat tacgtcctgc ttgctttgcc
    10201 ttataaagga attgatcggc tctttgcacc aagctttctt catcatcatt ttcctgcagt
    10261 tctgttaccc cgaaactgca tgttaaagat atactttggt gaaaattatg ctctgaaatc
    10321 agtatcctca acctttcagc cagattaaca gcatcaactt tttttgtatt tggcagaaga
    10381 ataacaaatt cctcacctcc ccatcatgca aaaatatcag tttttctgac tacctgcttt
    10441 ataaggccaa cagtttccag catgacttta tcacctgtaa gatgtccaaa acggtcatta
    10501 atatttctaa aatagtccaa atcaaatatt attattgata atgctgtatt gtaccttttt
    10561 agaagattta tccatcttcc aagctcttca tcaaacttaa aacgattgta taatccggtt
    10621 aggggatcta tagtggataa tacttctaaa tatttactat taatatattc caatcgcttg
    10681 taataattta ccctatagga ggttatggca ttcaaagaaa taacaagtat tgtataaaca
    10741 acagctgccg aaaattctga aaattgtgtt tggggcaaac gaaatttgga caaaattaga
    10801 aagcctattg taataaatag tgaagatgct aacatgtaaa ctaatctatt aggtataaga
    10861 aatataacta ttataataat tataactcca aatgtctgga ttataaaatt tggagattca
    10921 tattggtaaa agatggataa gtacgaaata gacaaggcaa tttcatatag cgtgaaatac
    10981 ataaatattt tagaataact ctttatatgt ccaatcccaa catatagtag aattactaaa
    11041 ataaaaaagg taagtctatt taaaaatatt attctaaaag taaatggatt atttataaga
    11101 aaatagtcag gtattgcgaa aaaaaggtag aacaccccga agcataatac tgcagggcgc
    11161 agatatttta taatacttag gatttcataa tcaatatatt caaattcaag aaatctatct
    11221 ttgaattctg ctaataatgg agatatattt tcgcgtatac ctgcactttt atttgtgtca
    11281 atcatataaa ctagccctta tattgttttt taaatataac gaggcaatgg atgtccctca
    11341 cctccatatg acaatgatgt gaaaaaatta cagatctaaa tctggtgtta gaaagcaccg
    11401 ccgccgccgc cacctccgcc gccacctgaa cttcctccgc tgaagccgcc acctgaacct
    11461 gttgatgaag aattacggga attagcaata gtagttgcag tcattactga tgattccaca
    11521 gtcttgatag tattgttgaa aactctatca agattgctga aatgcccgta ggacatgcca
    11581 taaaggtatg tcaactgagg attgccaaga tcatcctctt taaataccag tggcagttgt
    11641 tttataacct ctcttgctac gccaagtgat attgcgtaaa caaggtagtg ttcccaaagg
    11701 atgatggatg gcacttcagc ctgttccaat ctgctgaaat ccttgagaaa acgcttgaaa
    11761 gccttccaca ttgcatactg ttcattgccg taagtggaac gcctttttat cctggcagag
    11821 aaaagcagca tgataatgcc aagaggcaaa agagtaatac caactgtatt tttaaatatg
    11881 ccgggcataa gtataccgat aataataatt acgattgagg aaagtatgcc aatgatttta
    11941 cctttttttg ccgaatcatc aaagaactta ttcaattttg cctcattttc agctttttca
    12001 caccacagat catagtctgc tttgaaatcc aatgcattat ctttatctcg ggcaaaatct
    12061 tttatatcgt ccaataaaac agaatttcca tcaccaattt tattaataaa ccacttaatt
    12121 agaaaggcct catgggattt caaacttgtt aaaggctttt cagtgttcaa tgatatctta
    12181 tagtctttta cttctttgtt tttaaacaac ccttttttat ttactgtaac ctgctctagc
    12241 aaaaggtttt tcttccttac caggtccata agagtagcca taacatctct tgattgaact
    12301 ttacccatag tcatgagcaa actcatttct gcaggagtat attcaccagg taattccctg
    12361 taatacttac caaagaatct tctcttaaac tccctatcat atttcaggta gatgtaaata
    12421 actaatagta accaaatggg taaaagaata taaaatatta ctgttccaat aacattaaga
    12481 gctttctcct tttcctgctg ctttcttaac tgctccctgg cattttctct tttaagattt
    12541 gcctcttctg caagtttgcc ttcactttga agtatatcct gcagggcacc ttttgaaaca
    12601 atattttttg agtcgggaac aagtttaaca ggaaataata ccctggtttc aacaaacgtt
    12661 ccgggggcta cagctggcac tttgaattct acagtttttt catcaattat atcagattca
    12721 cctgttaaag gcccatgccc aaatactctt atatctcctt tcgaggctcc ctgaggaagt
    12781 attgtcttaa tgtggatatt atttagaggt gcctgccatt cagtacctat catttttctg
    12841 ttgaattctg caatatcatt atattttaca acagcatcaa ctaaagtata tttatatatg
    12901 aaggtttttt cttcattacg ggagggttcg tatactttaa attttgccat gctgctgttt
    12961 aaattatata aatatgttcc gggactttct gaattattcc tgacaacttc aacttgttta
    13021 ccatctttaa gtataaatac ctgctcatct tttattcctt tagttccgga caagtcaata
    13081 tcgaaagtta caccgttaaa attacctgaa aaactataag ttatgtgttc ctctatatta
    13141 gctgaaccgt cggtactaat atttatataa atattatact tcgaaataaa gtatttcctg
    13201 ttattctctg ctattgcggg ctcagcataa aaacatagta tcagaaatat ggataatata
    13261 aacgatatag atgatattac cctcagtata tacttttttc tttgatccat acatatccct
    13321 tctttcaaaa atttgattgc ccgggcgagt aattattact atgattctgt aacataaaat
    13381 catatttatt attattattt gttgtaatta tatcatagag atttttgaat gaaaatatac
    13441 cagtattaat aaatatagcc acttatatat ataaggcctt gcgaccattt acttattatt
    13501 tattccctat gggttggcaa aaattaccct tcagaaatgt gctttccatt ggcaacataa
    13561 caaagcagta atagtttaga taaataaata acaatacaaa ggtaataata tgattattaa
    13621 cttgatgaat gaagggagac tttaaagtgg cagtcttaaa atttaaagct gatgcttatg
    13681 gcgaaaaccc tacaaaaaca gtagtagaag cacgaaattt taaaataact atcgatgaac
    13741 cggaaaatct gggaggtacc gatcaagggg caaatcctgt ggagtatata cttgctgcgc
    13801 ttgctggctg tttaaatgtc gtcggtcatg ttgttgcaaa agagatggga tttgaacttg
    13861 gaagaatgca tattgaatta gaaggtgacc tggaccctgc aagatttatg ggtagaacga
    13921 aagcagagag agcaggctat aaggagatcc gggttaaaat tagtccggaa accaatatag
    13981 atcataattc tctagaaaaa tggcttgaaa caatagaaga caggtgccct gtaagcgata
    14041 caatacagaa tatcacacct gtaaaaatcg aattaaacac aactgccggt tattaaagca
    14101 gagagcttgt atgatgtatg gaaacggaac ttccagatgt attgcatagg cgcagagtat
    14161 ttatttgaat atttgaagaa aatattggta aggggcataa gcactttaac gatagaccct
    14221 gataaatata tcttcaaata ttaaatgtac cccatagtaa atgcgccctt gcacatttta
    14281 acatattgac atagaatggg tgattgtgta acaatcataa tatatataca aagtacaggc
    14341 tcaaatttac atacagtgaa attgccaaac gtaagtatat aaggattatg cataggggga
    14401 aagggtacat gaattacagt attatggcat atattatatc tgcaggagct gtagcacttg
    14461 ctgaaatggc tgacaaaaca cagttgatgg caatggcatt tgcatcaaaa tataaggtct
    14521 caaaagtgtc actgggagta tttttgtcta ttgttttcag ccaggggatg gcagttgcca
    14581 tgggtaatta tatggggcag catcagggtt taaatgtttg gatacaggct attgcttcat
    14641 tatcatttat tttctttggg ctttggacta tgcgtggaga aaagtcagaa gatatgaagg
    14701 tgcaaaattc taaattcggt gtagtaataa cagttgctat tgcatttttt atagcagaac
    14761 ttggagataa aacacagctt gccactattg cttttgctat taagtatcca tcaaatccta
    14821 ttagtgtttt gcttggtaca ataacaggta tggttgtggc taatgctctt gggatatttg
    14881 ttggagttgt attatgcaag agaattcctg aagaaaaatt gaaattcctt tcagctggag
    14941 cattcatatt atttggtttt attggtgtgt atcaagttat ggcagacaag ttgaatttta
    15001 ccactggcac aattgcagtt attatgatac ttatagcact tataacggga acagctatgt
    15061 attttatttt caagaaccga gaaatacccg aaaaacgtgg atttaatcaa tattgcagaa
    15121 taaagggccg gtaacctgat agctactgat ataaaagagc gggatttggt tatgggtgca
    15181 tttcctttat attaaaatat gcacctcttt attatttggg cgcattttct tcctatgata
    15241 caaaatgcac atttattatt tgtactattg aaaactcaca gtaaaaatgc tattattgga
    15301 aatacgctta cagtgttttc aatttaaggg aatatacctt aaagctaaaa aagtagtact
    15361 tgctaaataa taatatataa gtattattaa gatgttattt ctaaacatta tatgtaaatg
    15421 gtgcataatt aaattaaacg aggtgtaaaa atatgggtaa aaatgtgtcg cttctgcttg
    15481 tgggtatcgg agggtatggc aataactatg ttaaaggtat tcttaataat atgggtgata
    15541 tggccaaaga taggtttaca ataaagggca tagtagatcc taatccttcg ggatgtcaat
    15601 atattaatga acttaaggat atgggtattc ccttttataa ttcacttgat gaattctatg
    15661 cagttgattc tgctgattta gcagttatat cttcaccaat tcagtaccat gcaccccaat
    15721 cttgttgcgc acttgaacat ggcagcaatg tattatgtga aaaaccaata tgtgcaacaa
    15781 ttcaagatgc actaaagatg attgatgcaa gagataaatc agggaaattc cttgcagttg
    15841 gttatcagtg gtcttatgaa gaagccatgt tggatttaaa aagggacatc ataaatggta
    15901 aattagggag accaaaacgt ttaaaaacca ttgtactttg gccaagagat aaaaagtact
    15961 atacaagaag cagctgggct ggcagaaagc aggacgaccg tggtaactgg attcttgaca
    16021 gtgttgcaaa caatgctaca gctcattatc tacataatat gttttttgtt ttaggtaaca
    16081 aattggatac cagcgcatat ccagccagct taagagctga actatataga gctaatgaaa
    16141 tcgagaactt cgatacttca gttatacagg catatactga tgatggagtc gagattttgt
    16201 tttatgcaac ccatgcagta aaagagacca aaggtccaat tttccaatat gagtttgaaa
    16261 acggtatagt taactttgga ggagaaacag gggaaaaaag aataatatct gcaacattta
    16321 ataatggaac cacaaagata tacggtgaac catactcaac cgaaatcaga aagctttggg
    16381 tatgtataga tgcagctgca ggtattggag atgaaattac ctgcaaggca gaaacagcaa
    16441 tacctcagat tttatgtatt aatggtgctc aggaatctat gcctgaaata gtgaaatttc
    16501 ccgatacttt gataaaaaga gaaggagagc ctgagttgac ctgggttgaa ggactctctg
    16561 aatctttaat acaatgctac aacaataata ctgttccgac tgctatgggc attagctggg
    16621 cgaaaaccgg aaaagagatt aatttaaaag gttattgcag tataacccta tagacaagac
    16681 aggcaaataa tatgctataa taatgaggat actcttcaaa gttggaggaa ttccctttac
    16741 ttgagagtgg gtgtcctttt atagaaaatc atatgtcagg tggggtcata ataaataata
    16801 tatatactaa atatacaaaa tgggcgaaat ataccaaaca caataaatat agcatgttta
    16861 aagtttttat gtttgcttat tgggcaatga tgctaagcat ttcaggaatg tatgccatgt
    16921 acattgctca aataggcttt tctaagaagg agataagcat tgctgttacc atatttacct
    16981 tcgctgcgct gattggacaa aatgtatttg gctatcttgc cgaccgcttc aaatgcatca
    17041 aaaagatact cctggttgca attagtgtag gaataatcgc atcaattgca ctaaactttt
    17101 ctaaacagag ctggttcata agtatactta tatttctatg gggatttttt ttatatggaa
    17161 cggtacccct ttcagaagca tggtatattg gcattctgaa agctaatgga gaccaaaggg
    17221 aatttggtaa aatcagaggt atagggtcta ttggatatgg gatctctgga attattcttg
    17281 gccttttgct gcaaaatata ggctggaatc tttatagctg gtatatttta ataagcagct
    17341 gcgctgtttt attagtaata accttaatga gtgaaagcaa agagattgct ttatataaag
    17401 caggcggcgg agacggttgc ggggatacta ataatgcttc ttttaaagag gcttttgagc
    17461 agattataac aattaaatct ttaagaacta taataataat agtatttata tatagctttg
    17521 ttctaaaagg catatatagt taccttggtg t
//
LOCUS       DAOSDD010000077        17524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1315_length_17524_cov_4.615490, whole genome shotgun sequence.
ACCESSION   DAOSDD010000077 DAOSDD010000000
VERSION     DAOSDD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17524
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_17524_cov_4.615490"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..808)
                     /locus_tag="PK733_08585"
     CDS             complement(425..808)
                     /locus_tag="PK733_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10635.1"
                     /translation="MRDVRYADKNKIIDILEEGFKLLDEMLEKDRVIHYIESCLRYIL
                     SVRNDIDKDEMVRIASQISIEGSELVMTIAEQLRQEGRQEGIKEGVIRITKNAIVKGM
                     KVEDIIKLTGLTKGDIDKIRKEMLQ"
     gene            complement(1118..2173)
                     /locus_tag="PK733_08590"
     CDS             complement(1118..2173)
                     /locus_tag="PK733_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10636.1"
                     /translation="MKKKIIIFSIIICVLCIAFAGYAWVDSEYGIGMKDVIYIRNNPD
                     SLKKTEVLIPDVKNADKIEITNSSEIFDDGSLIFNVKSVYDNKEIGTFLYKPWVEKRS
                     IFYRLDNGDVILGKMNIYSSKENKIIRLVEGTDMRTVFDYDVYENSLAVIAKSHSGSS
                     DICIAIKDMPDGEFKVIDTFAYPPFNYAEIVYLGWGDDGKLYYDYAQNDTPVIKVYDM
                     KTNSSQIFMNEARSPNISPDRKHIVVSIADSFNKNKQTKSGIQLLDLQKNIKLTDLNC
                     TWRIFWDSEYLIVSNVDDITKWEVYDLSDSGKKVKEFNFEEQPYEMKVSNNILKIKSY
                     KFDKSVITESEKDVVVE"
     gene            complement(2193..2474)
                     /locus_tag="PK733_08595"
     CDS             complement(2193..2474)
                     /locus_tag="PK733_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10637.1"
                     /translation="MRGKVLTSSGAYARYTRISGATKGTDINYGASYSYYVMEDGAPF
                     PDEVVYGINYYIARINTGNITLNFNNGAITKGVTVSNNTDSTVSDTTLP"
     gene            complement(2456..3343)
                     /locus_tag="PK733_08600"
     CDS             complement(2456..3343)
                     /locus_tag="PK733_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ10638.1"
                     /translation="MLKLIDGILQQFRICFKREETFNWFVIIVVGMMLRIEMKGITTI
                     IGCLGLDPGCYETMLHFFRSKAYDLEQVKSKWQSMVLGHIQPVMMDGYMMLIGDHIKV
                     SKEARHMPGVKKLHQDSENAGKAEYIFGHQHGMLGILAEGKTNQCIPMDIELQDRIDE
                     ITCLKSDTRVTQEALKKLKDENNSIMKMIQMAGRFVKEKGHKAIVLLDAFFTSGDAFH
                     AVDQTNQELGAHDVVLITRAKANTVAYEEPVTQEMQQDRICILRYGWVRIIGISREIR
                     KYCLKEVPLMGMVRCVEKF"
     gene            complement(3479..3673)
                     /locus_tag="PK733_08605"
                     /pseudo
     CDS             complement(3479..3673)
                     /locus_tag="PK733_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(4262..5038)
                     /locus_tag="PK733_08610"
     CDS             complement(4262..5038)
                     /locus_tag="PK733_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ10639.1"
                     /translation="MKTRIYIIKLNSIISYFAILVAFTTVLSLLGITGMKAVEVFASV
                     RSIPIYSVDYTEKKASITFDCAWGSDDIPDILNTLERENIKATFFIVGEWAQKFPQMV
                     KLIAEKGHDVANHSHTHLRMGVLDEERIYKEIVQCNNKLEEISGKKVELFRPPYGDYN
                     DLVVKTAGKAGCYTIQWNVDSLDWKPGISKEEIMNRITKRIQPGSIILFHNDTPHTAK
                     LLPTIITDLKKNGYSFLPVSELIMRENYYIDHEGVQKRKK"
     gene            5301..8900
                     /locus_tag="PK733_08615"
     CDS             5301..8900
                     /locus_tag="PK733_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HOJ10640.1"
                     /translation="MVETENSEFLLNEEIIRREAAVQPIFDLGRKLYFENSILYLDVN
                     LEERSLIGCIKDGNSLYTSEIQFDKEGNLQRYGCTCDIFKNYLVACPHIVAMMQAIYG
                     NLKIIAYLTSERNTAKALNMKVPLNPVIVLNKVSFQDKPAQRPETVELSKRLPNSPKK
                     TVELFKTLKDKYQIQTDYQKNTKHTDNIEELKQGKTLKQALKQVLKKSEAKPVFSLDS
                     NAKEFFQAFNNFVSVKPVGKNKSTKKVKLIPTYYINNSYGGTTHWLEFTTGINKTYIV
                     KNIVQYINCIAFRKPCKFGKSFNYDPETMEFEEGASKKMAELLTEICIDNKHLNSSDY
                     IYYGTSYQSRRIVLSNSRLLEFLEIMKEHNQSFRLVIDELALDNMEIKTETPEIKMKL
                     DRINGGFKLSFDILSDKIFMLDYEGRFIFYENSIYKLTPENAIIMSPIINYVNASRRP
                     EVLIPDNEISNFYSNIAPHVENIGEIKIHKDIKTEFIREELSASIYFDRIKHKGKDGI
                     SARLEYAYGDITINPLMLQNELYLDDKIIIRNKHQENQIENLLINAGFSESRDVYCLM
                     DEELMLDFITNELPVLKDMAEIYYSESFKTIKLSSNINFGAGVRLSTDGQWLEFTIKH
                     DGIDSEELMELLSSYRLKKRYYKLNNGSFVILNDHQINTIADVIAKLELTNKDISKEI
                     IQLPRYRAMYLDSLAGETEDFHIERSSQFKNLVQNIKEPGDMEFEVPASLKGILRDYQ
                     KTGFKWLKVLSEYGFGGILADDMGLGKTLQVITFLLYQKQESSEKPSIVIAPTSLVYN
                     WEEEVKKFTKDLRVAVISGTVNERQQQLIEIKDADLVVTSYALIKRDIELYENIEFSH
                     CFLDEAQYIKNPGTLNAQTVKRLKARNYFALTGTPIENSLTELWSIFDFLMPGYLFSH
                     NKFNQIYETPIVKKNDSEALKGLARHISPFILRRMKKDVLKELPEKTEIRVSCTMEEN
                     QKKAYMAYLLKAKNIFEAEVNENGFEKSQIKILALLTRLRQICCHPSLFIENYNGGSG
                     KVELFFDLINGAIEGGHRILVFSQFTSMLAIIKQELDAQKEGYFYLDGNTDPQLRIKM
                     VSAFNKGENNLFLLSLKAGGTGLNLTGADTVIHLDPWWNPAVEDQATDRAYRIGQKNA
                     VSVYKLVTKGTIEEKIYGLQERKKELINSIIKPGENFLTKMSFEEVKSLFETE"
     gene            9017..9736
                     /locus_tag="PK733_08620"
     CDS             9017..9736
                     /locus_tag="PK733_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOJ10641.1"
                     /translation="MIIAGLAGYGSYQVQTADLIYTILNSYDKKTSFLDCKSLEEFST
                     SKLKAYINEIGKNGTDYLILKIDLSDFDPELFEYLQEYLQFDIILYNNETDKFLDFDA
                     SAYMESIEKLMPFLSEKGIVIINEDFKEAWLPVDNFEYQIVTYGFNLKAGVTTSSISD
                     TLFGSELMFYQQQTIRTINGKIITPQEYRVYIEKKIMDIYIILGAVTFAIVSGVDPSR
                     NLSIIMPYEAMNVNIASNNEC"
     gene            9985..10674
                     /locus_tag="PK733_08625"
     CDS             9985..10674
                     /locus_tag="PK733_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ10642.1"
                     /translation="MVGNLIENNIVTVSGKVVSEMEYSHEIYGEGFYTFILEVPRLSD
                     SSDKIPVTISERLISKQELKEGCMVEIEGQFRSYNNYAREGNKLLLTIFAREINFLED
                     DGKIKNPNQVYLNGYICKKPIYRMTPFGREITDVLLAVNRPYNKSDYIPCIAWGRNAR
                     YSESLSVGDNIKIWGRIQSREYQKKYDSGEIITRTAYEISISKMEVCNKEQTDYQELP
                     YTENEETDDHT"
     gene            10881..11291
                     /locus_tag="PK733_08630"
     CDS             10881..11291
                     /locus_tag="PK733_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10643.1"
                     /translation="MIKVIYGEKGTGKTRILVESSNVMAERNYGHVVFIEDSNQSMYK
                     LIHKVRFINISEFPIYGEDGFIGFICGIISQNYDTKGIFIDGLTYILKKDVNDLEPFF
                     SKLKVIASKFNVEFILSVNGNAENIPGFLKEYIL"
     gene            11413..13935
                     /gene="priA"
                     /locus_tag="PK733_08635"
     CDS             11413..13935
                     /gene="priA"
                     /locus_tag="PK733_08635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOJ10644.1"
                     /translation="MDNIASVIISNSTREFDKEYHYLIPDNMKGIIKSGMRVIVPFGK
                     ANTLREAFVFRTFESNEAMGLTGLKYIKKLIDKTPALGENMINMAIWMKERYICTYYD
                     AIKCMLPSGTKVKTYKIVKLEGSSNTDEIQVNNNKILTDENIYKNIDKNEELIIKTLA
                     QSGNQCEMEELKGIINIKNISRYIKKLSDKGLICIQEGYTARVREKFVKAAYAAIPAE
                     EIAEIIDSNRIKRIQQIKVLEILMENEYVAVQDIVRFAGVSQGVLDTLKKYGYIDFRE
                     IEIKRDPFANREYKRTEPLKPTLEQEAALKILKEQKDRQLFAEDLLYGVTGSGKTEVY
                     LQLIQYALDKGEEAIVLVPEISLTPQMVERFKGRFGNQVAVLHSRLSLGERYDQWRLI
                     KDGKIKVVIGARSAIFAPLANIGLIIIDEEHEASYKSEITPKYHAADIARYICKHRNA
                     VLLLGSATPSVETYYKAVNGEIGLINMEERANNMTMPHVEIIDMREELNQGNRSVFSR
                     KLAVEIDKNIAGAEQTIILLNRRGYSPFVLCRSCGYVAKCINCSITMTYHAVGERLTC
                     HYCGYTIKVPEKCPKCGSGYIRHFGTGTQKLEEETKKQFPGASVIRMDMDTTGYKNSH
                     EEILGKFRDKNINIMVGTQMVAKGHDFPNVTLVGVLAADSILNIGDYRASERTFQLLT
                     QVAGRAGRGELPGRVVIQAYNTDDFSILAASRHDFISFYSKEIKIRREMSYPPFTNIA
                     TAVLSGRYDKNVFECSKRFEDILNSLFIYYEIHKDSYEILGSMRPPIGKIKNKFRWRI
                     VIKSTDINTLVRVLSGASDEYYKLKYKEKAQLSIDINPVSML"
     gene            14064..14522
                     /gene="def"
                     /locus_tag="PK733_08640"
     CDS             14064..14522
                     /gene="def"
                     /locus_tag="PK733_08640"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ10645.1"
                     /translation="MAIRKIRKDGDEVLRKKSKKVEVINDKIISLLKDMAETMYESDG
                     VGLAAPQVGILKRVVVIDVNDDRGLIELINPEIIFSEGEQNESEGCLSVPGVYGQVKR
                     PARVIVEALNSKGEKITVEGSKLLAVALCHEIDHLDGMLFTDKVINYTHK"
     gene            14555..15502
                     /gene="fmt"
                     /locus_tag="PK733_08645"
     CDS             14555..15502
                     /gene="fmt"
                     /locus_tag="PK733_08645"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ10646.1"
                     /translation="MKIVFMGTPEFALPSLDMLVREGHEIAAVVTQPDKPKGRGQKLC
                     CPPVKEYADKNGIIVLQPYKVKTPDFIYSIKALEPDLLVTAAYGKILPLEILNIPKYG
                     CINVHASLLPEYRGAAPINWAIINGELKTGVTTMLTDIGMDTGDILLKKEMNIPDDMI
                     AGELHDKLAVFGAECLKETLAKLKDNTLYRIPQQHEKASYAPMLKKDVGLISWGKPSR
                     EIHNLVRGTNPWPVAYTFYKEKRVKIWKTQTELRLENENDACYDAYQPGTICKRDKDG
                     IFVKCGEGFIKILELQFDGCRKLCSDECWHNFESGESLG"
     gene            15554..16255
                     /locus_tag="PK733_08650"
     CDS             15554..16255
                     /locus_tag="PK733_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOJ10647.1"
                     /translation="MGYFYGFDKYYLMLVLPAFIFSLYAQFRVKSTFSKYQRLSNRSG
                     YTGADVARAILDKNGLGNVSVQMVPGQLTDHYDPRTNVVRLSQSVYGSTSVAALGVAA
                     HETGHAIQHKVGYRPLVLRTTLVPIANIGSMAGPYLAIFGLLFDFLQLAYIGIILYAG
                     AVAFYLITLPVEFNASTRAIEALETTGILTWDESEPAKKVLKAAAMTYVASAAVAIAS
                     LLRLLLLTGNRRRND"
     gene            16286..>17524
                     /gene="rsmB"
                     /locus_tag="PK733_08655"
     CDS             16286..>17524
                     /gene="rsmB"
                     /locus_tag="PK733_08655"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOJ10648.1"
                     /translation="MPVDLPREIALKILYEINEKGAYSNIAINKHVENPGLRDIDKVF
                     ITGLVYGTIKWRLSIDFVIGQFSKLPMKKLSPWILNILRLGIYQILYTDKIPVSAACN
                     ESVSLALKYGHKASGGFVNGILRNVARNKDNIVYPDKNADLIRYLSIKYSHQEWMIKK
                     WAKLYGEEFTENLLQSNNENPVFSIAVNTLKISREGIRQKLHEEGIDCIEGKYSDNAL
                     ILENPAGIVHSYLHKEGYFQIQDEASMLVSKVLDPAPGELVIDVCAAPGGKTANIAQM
                     MNNCGTVVARDIHPHKIKLIEEASKRLGLNIVKTQLFNAEEIDPSCKSRADRVLVDAP
                     CSGLGIIRRKPDIKWTRTPEDMKTIPPLQFKILCTAAEYVKPGGVLVYSTCTIMPDEN
                     IDVVSKFLELNSDFKMVGFET"
BASE COUNT         5993 a         2791 c         3250 g         5490 t
ORIGIN      
        1 ttatgcggtt cataatctct tccttgctaa taccgggctt ccagtctaag ctatcatgta
       61 tgcatatgaa agtaaaccga gaatatgtca ataatattag atttaccaat caatattaat
      121 ttccttatct ttaatgtaag ctaaacttta tctataaaga agcctcctat ctgcttcttt
      181 atagataaag cttagttttg ccaattttta actttttata atacttcctt tacaggctga
      241 gtcttacgat tttcaatagt aagtttttta tttttcccta ataatatgtt tttattatct
      301 tataattgcc aagtattaaa tttgcttaac actgtgaaat tatatcaatt ttatctttca
      361 aaaccatatt tctataactt ctaccatcca tcgtatctat tacatttctt gcctagtact
      421 cacattattg aagcatttcc tttcttattt tatctatatc acctttggtt aaccctgtaa
      481 gtttaattat atcttctact ttcatccctt ttactattgc atttttagta atccttataa
      541 ctccttcttt tataccttct tgtctgcctt cttgtcttaa ttgttcggct attgtcatta
      601 ccaattcact cccttctatt gatatttgac tagctattcg taccatttca tctttatcaa
      661 tatcatttct tacacttaat atatatctta agcatgattc aatataatgt attacccgat
      721 ccttttctaa catttcgtct aagagtttga atccttcttc taatatatct atgattttat
      781 ttttatctgc atatcttaca tccctcatta tttttagtat taccctagtt atggactcta
      841 attttatatc ttcatcttat gtccatgtta taacccccta tttttcaaga aaagctgtat
      901 ttctgataaa atgaaaaaac tctattctct tttcttgata atttaaatta atcatcttta
      961 tcatccctta ataatacttt tcataaattt attatactta ttaaagagtt cttcacaact
     1021 attatatcaa acatatgtgt ctcaaaaagc ggaaaataag cttgtattaa ttgagcacaa
     1081 gcctattttc tgcttcaatt gcgtttaaaa atcaggctta ttcgacaacc acatctttct
     1141 cactttcagt aattacagac ttatcaaact tatatgattt tatttttaaa atgttgttac
     1201 tgactttcat ctcatatggc tgttcctcaa aattaaactc cttaactttt ttaccgctat
     1261 cactcaggtc gtatacttcc cattttgtta tgtcatctac attgctaaca atcaaatatt
     1321 ctgaatccca gaatattctc catgtacaat taaggtccgt aagcttaata ttcttttgta
     1381 aatccaaaag ctgtattcct gatttagttt gtttattctt attaaaagaa tcagctatag
     1441 aaacaacaat gtgttttcta tccggagata tattaggact cctagcctca ttcataaaaa
     1501 tctgtgaact atttgttttc atatcatata ctttgattac cggagtatca ttttgtgcat
     1561 aatcatagta taattttccg tcgtcacccc atcctaggta aactatttca gcataattaa
     1621 atggaggata agcaaaggtg tctattacct tgaattcacc atccggcata tccttaatag
     1681 ctatacatat atcagaagaa ccagaatgac tctttgcaat aaccgcaagg ctgttttcat
     1741 aaacatcata atcaaaaaca gttctcatat ctgttccttc aactaaccgg ataatcttat
     1801 tttctttgct gctgtaaata ttcattttgc ctaaaattac atcaccatta tccaacctat
     1861 aaaatataga tcttttctcc acccaaggtt tatataaaaa tgtgccgatt tctttattat
     1921 cataaacgct ttttacatta aaaattaagc ttccatcatc aaaaatctca gaagaatttg
     1981 ttatttcaat tttatctgca tttttcacat caggtattaa aacttctgtt ttctttaaac
     2041 tatccggatt attcctaata tatataacat ctttcatgcc tatgccatat tcggagtcta
     2101 cccatgcata gcctgcaaaa gctatacata atacgcaaat aatgattgaa aatataatta
     2161 tctttttttt catactaatc cctccctaat atttatggta gtgtcgtatc acttactgta
     2221 ctatcagtat tattacttac tgtgacacct tttgttattg ctccattgtt aaaattcaat
     2281 gttatattac ctgtattaat tcttgcaata taataattga tcccatacac aacttcgtca
     2341 ggaaacggag cgccatcttc catcacataa tatgaataag atgctccata atttatatca
     2401 gttccttttg tagctccaga tattcttgta tatcgtgcat atgcaccaga tgaagttaaa
     2461 acttttccac gcaacgcacc atacccatca gaggcacttc tttcaagcaa tatttccgga
     2521 tttctctgct tataccaatt atcctcaccc atccgtatct caaaatgcag atgcggtcct
     2581 gttgcatttc ctgtgtcacc ggttcctcat atgcaactgt gttggctttt gcgcgtgtga
     2641 ttaaaacgac atcatgggca cccagttctt ggtttgtttg atcaacagca tgaaaagcat
     2701 ctccacttgt aaagaaggca tctaacagga ctatagcttt gtgccctttt tcctttacaa
     2761 aacgaccagc catttggatc attttcataa tgctgttgtt ttcatccttc agtttcttca
     2821 aagcctcctg agtaaccctg gtatcactct ttaagcaggt gatttcatca atcctatcct
     2881 gtagttcaat gtccattggg atgcattgat tcgtttttcc ctccgcaagg atccccagca
     2941 taccatgctg gtgcccgaat atatactctg ctttccctgc gttttctgaa tcttgatgaa
     3001 gcttctttac tccaggcata tgtcttgctt ccttggatac tttgatatgg tcaccaatca
     3061 gcatcatata accatccatc atgaccggct gaatatgtcc aagtaccatg ctttgccatt
     3121 tgcttttaac ttgttccaaa tcatatgctt ttgaccggaa aaagtgtagc atcgtctcat
     3181 agcaccctgg atcaaggcct agacacccaa taatcgttgt aatacctttc atctctattc
     3241 tcaacatcat acctaccaca ataatcacaa accagttaaa tgtttcctcc ctcttaaaac
     3301 atatgcgaaa ttgctgtaga attccatcta taagcttgag cattgacgtt gaccccccgg
     3361 ttttttatta cttcacaatt ttaatttacc atgggtgttc ccgtaatctc aagcttatta
     3421 catatgcaag ttctggtaag aacttttaac tctcaagaaa ttttctgcat tcaggtattt
     3481 ccaagaagca tttaagcatg ctgaagttaa gagtcttacc aaaacgcctt ggccttgtga
     3541 agagtataac cttgctcaga ttattcatta tttcccttat aagtagggac ttatccacaa
     3601 agtatccatt ctttgttata agctctttaa agttatctat tccgataggc atctttttta
     3661 ataaattttg cataaacaca ttcctccagc ttcacttgtt ttcttttagc tgcatttgat
     3721 acatctgagc ctcatttaaa tcttctgatg gctttattta gtaccatttc atctgttact
     3781 aatattatta taccattcat atagctttta taaagggatt tcagactgat ttcccaataa
     3841 aattgttact cccatgaatt aagcaatgat gtatatactg atagaaaaat tagaagacag
     3901 ttatcctgta ttattgctaa atcaagataa ctgtcgccat aatttatctc ttatactctc
     3961 tttatactat tattctcaaa aagttaacaa aaaacaaact ttcatataca aatccatgta
     4021 gtgcaagcgt tacatggcac ttttcgagaa tatattgtat cttaattcat cgcagcgcac
     4081 acttatttgt ttgtggctat acgctgcggt ataatatatg cctatataac gaggcaatga
     4141 agatttcccc cacatctata ggtggcgggt acctatttgc atttcaagcg gcgagcatat
     4201 ctcccgcctt gttgaaacgt gctgaggtaa gaaaattttt ctactgatgc gttataagca
     4261 cctacttctt tctcttctgt accccttcat ggtcaatata atagttttcc ctcattatca
     4321 gttccgacac cggcaaaaag ctgtatccat tctttttaag gtctgtaatg attgttggca
     4381 aaagtttggc agtatgaggt gtatcgttat gaaataaaat tattgaccct ggttgaattc
     4441 gttttgttat gcggttcata atctcttcct tgctaatacc gggcttccag tctaagctat
     4501 ctacattcca ttgtattgta tagcagcccg ctttccctgc agttttcact accaaatcat
     4561 tataatcacc gtaaggaggt ctgaaaagtt caactttttt gccgctgatt tcttcaagct
     4621 tattattgca ttgaacaatt tccttatata ttctttcttc atccaagaca cccattctaa
     4681 ggtgtgtatg agaatgatta gcaacatcat gacctttctc ggcaattaat tttaccatct
     4741 gaggaaattt ttgtgcccat tcaccaacta taaaaaaagt agctttaata ttttctcttt
     4801 caagagtatt aagaatatca ggtatatcat ctgaccccca ggcacaatca aatgttatgg
     4861 atgctttttt ctcagtataa tcaacagaat aaattggaat gctcctaact gaggcaaaaa
     4921 cctccacagc tttcattcct gtaatgccta atagtgataa aactgtggta aatgcaacta
     4981 gtattgcaaa ataggaaatt atgctgttca attttattat ataaattctt gttttcatcc
     5041 tataccaaac tccattctct tgatattaat cacattaatt aatatttaat aaataaatat
     5101 tatatgcata tttatataat aattagaatc tattttgtgg tgagaaatgt ccctgttaca
     5161 aatatataaa catacataca acttattaca agatcggaaa tataataatg aataaatgca
     5221 ccttattgta tgtattgtat aactcctgta aattggttat aatctaacaa gtagcaacta
     5281 gcatttatag gagaattggc atggtagaaa cagaaaacag tgaattttta ctaaatgaag
     5341 aaataataag gcgtgaagct gcagttcagc ctatatttga tttaggacga aaactttatt
     5401 ttgaaaattc catactatat ctggatgtga atctggaaga acgaagctta attggatgta
     5461 tcaaggatgg taacagctta tatacttctg agattcagtt tgataaagaa ggcaatttac
     5521 aaagatatgg ttgtacatgt gatatattca agaattattt agttgcatgt cctcatattg
     5581 tggcaatgat gcaggctatt tacggaaatt taaagattat agcttattta acttctgaac
     5641 gaaatactgc aaaagcttta aatatgaaag taccactgaa tccggttatt gtattaaaca
     5701 aggtcagttt tcaagataaa cctgcacagc gtcctgaaac tgttgagctt tctaagagac
     5761 tgccgaatag ccccaaaaaa actgtggagc tttttaaaac attaaaagac aaatatcaga
     5821 tacaaaccga ctatcagaaa aatacaaagc atacagacaa tatcgaagaa ttgaaacagg
     5881 gtaaaacttt aaagcaggcg ctaaaacagg ttctaaagaa aagtgaggca aaacccgttt
     5941 ttagtcttga tagcaacgct aaagagtttt ttcaggcttt caataatttt gtatcagtta
     6001 agccggtcgg aaaaaataag agtacaaaaa aggttaaatt aataccgact tactatataa
     6061 ataatagtta tggagggaca acccactggc tggaatttac tacaggaatc aataaaacat
     6121 atattgtaaa aaatattgta caatacatta actgtatagc cttcagaaag ccatgtaaat
     6181 tcggcaagtc attcaattat gatccggaga caatggagtt tgaagagggg gcctcaaaaa
     6241 agatggctga attactgact gaaatttgta tagataacaa acacttgaat tcttcggatt
     6301 acatttacta tggtacatca taccagagta ggaggattgt tttatcaaat tcaaggctgt
     6361 tggaatttct cgaaattatg aaagagcata accagtcttt cagattagtc attgatgaac
     6421 ttgccttgga taatatggag ataaaaacag agactccaga aataaaaatg aagctggata
     6481 ggattaatgg aggcttcaaa ctctcttttg acatattaag tgataagata tttatgctgg
     6541 actatgaggg taggtttatt ttttatgaaa acagcatata caaattaact cctgaaaatg
     6601 ctataatcat gtctccgata attaattatg ttaatgccag caggcggccg gaagtgctta
     6661 ttcccgataa tgagatctcg aatttttatt ccaacatagc accgcatgtg gaaaatattg
     6721 gtgaaattaa aatacataag gatattaaaa cggaatttat aagagaggag ctgtcagcaa
     6781 gtatatattt tgacaggata aaacacaaag gaaaggatgg aataagtgcc cgactggagt
     6841 atgcatatgg tgatataact attaatccac taatgctgca aaatgaactt taccttgatg
     6901 ataagattat cataagaaat aaacatcaag agaaccaaat agaaaacttg ttgataaatg
     6961 caggatttag cgaatctagg gatgtttact gtctgatgga cgaagagctg atgttagatt
     7021 ttattactaa cgagctgcca gttttgaaag atatggcaga gatttactac tccgaatcat
     7081 tcaaaacaat caaattaagc agtaatataa atttcggcgc tggagttaga ttaagtacgg
     7141 atggtcagtg gctggaattt actattaaac atgacgggat agatagtgag gaactaatgg
     7201 agctgttatc ttcatataga ctgaagaaaa ggtactataa gttaaataat ggttctttcg
     7261 ttatactaaa tgatcaccaa ataaatacta tagctgacgt aatagcaaaa cttgagttaa
     7321 ctaataaaga tattagcaaa gaaatcatac aactgccccg ttacagagcc atgtacctgg
     7381 atagtcttgc aggagaaaca gaggattttc atatagaaag gagcagtcag tttaaaaatc
     7441 tggttcagaa tataaaggag ccaggtgata tggaatttga agtacctgca agcttgaaag
     7501 gtattttaag agattatcag aaaacaggat ttaaatggct gaaggtcctt tcagaatatg
     7561 gatttggtgg aatattggct gatgatatgg gtttgggaaa gaccttgcag gttattacat
     7621 ttcttcttta ccagaaacaa gaatcatcag aaaagccttc tatagtaatt gctccgacct
     7681 cccttgttta caactgggag gaagaggtta aaaagtttac aaaagacctg agggttgctg
     7741 taatttcagg aacggtaaat gaaagacagc agcagttgat tgaaataaaa gatgcggatc
     7801 tggttgtgac atcatatgca ttgataaaga gagatattga attgtatgag aatattgagt
     7861 tttcacactg ctttttagat gaggctcagt atattaagaa ccctggaaca ttaaacgcgc
     7921 aaacagtgaa aagacttaag gcaaggaatt attttgcttt aaccggaact cccattgaaa
     7981 actctctaac agaactctgg tctatttttg actttttaat gccaggatat ttgttttctc
     8041 ataataaatt taaccagatt tatgaaactc caatagtaaa aaagaacgat agtgaagcat
     8101 tgaaaggatt ggctaggcat ataagcccat ttattttgag aagaatgaag aaagatgttt
     8161 tgaaagaatt gccggagaaa actgaaatcc gtgtatcctg cactatggag gaaaaccaga
     8221 aaaaggccta tatggcttac ctgctaaaag caaagaatat ttttgaagca gaagtaaatg
     8281 aaaatggatt tgaaaagagc cagataaaga tattggcatt attgaccaga ttgaggcaaa
     8341 tttgctgtca tccttcatta tttatcgaaa actataatgg aggcagcgga aaggtagaat
     8401 tgttttttga tctgattaac ggtgctatag agggaggaca cagaatactt gtgttttcac
     8461 agtttaccag catgcttgca ataattaaac aagaactgga tgctcaaaag gaagggtact
     8521 tttatcttga tgggaatacg gacccacagt tgaggattaa gatggtaagt gcatttaata
     8581 aaggagagaa caaccttttt ttactatcac taaaagcagg agggacaggc ctgaatttga
     8641 caggagctga tactgtaatt cacctggacc catggtggaa tcctgcagta gaagatcagg
     8701 ctactgacag ggcatacaga attggtcaaa aaaacgctgt ttctgtttat aaactggtaa
     8761 caaaaggcac aattgaagaa aaaatatatg gattacagga aagaaaaaag gaattaatta
     8821 attcaataat aaaaccaggt gaaaactttc tgacaaaaat gagttttgaa gaagttaaaa
     8881 gtctctttga aactgaatga aaatattcgt tttttatcct atgatacata aaatcattaa
     8941 aaaatgcaca ttatataaat tgcgcaaaca aaaccgggtt tgatgtccaa agaaatacga
     9001 gaaacggggt tttttgatga ttatagctgg attggccggt tatggcagct accaggttca
     9061 gacagcagat ctgatctata ctattttaaa ctcttatgac aaaaagacca gctttttgga
     9121 ttgtaaaagc ttagaagaat ttagtacatc aaaattaaaa gcatatatca atgaaattgg
     9181 gaaaaacggt actgattatt taatacttaa aattgatctt tctgattttg atcctgaatt
     9241 atttgaatat cttcaggaat accttcagtt tgatataatt ttgtataata atgaaacaga
     9301 taaattccta gactttgatg catctgctta tatggagagt attgagaagt taatgccatt
     9361 tctttctgaa aaaggtattg taataattaa tgaggatttt aaagaagctt ggttaccggt
     9421 agataatttt gaatatcaaa ttgttactta cggcttcaat ttaaaagcag gggtaacaac
     9481 ttcaagtata tcagatactt tgtttggcag tgaacttatg ttttatcagc agcaaacaat
     9541 aagaacgatt aatggaaaaa taattactcc tcaggaatac agagtatata ttgaaaaaaa
     9601 aataatggac atatatataa ttcttggcgc tgtaactttt gcaatagtca gtggcgttga
     9661 tccaagtcgt aatttatcta ttattatgcc ttatgaggct atgaatgtaa atattgcatc
     9721 aaacaatgag tgctaatgct tcttctaata atttcttcaa gaataaaata tgcacccaca
     9781 tccaggtttt taattgaata tgttaatatt ctgtaatata atgtaacata aagtcatgga
     9841 gtatgttatt agaagtacat ttttagaagt atattatcaa tagaaaaaat aaaaatactt
     9901 ctaaatttat ctctctatga atagtattaa attagattaa ttttgcagat atacatgaga
     9961 gaaagagaga ggagtggggg tcggatggtt ggtaacctca ttgaaaataa catagtaaca
    10021 gtttcgggta aagtggtttc ggaaatggag tacagccatg aaatatatgg agaaggattt
    10081 tacactttta tccttgaagt tccgcggctt agtgatagca gtgataaaat accggtaact
    10141 atatcagaac ggttaataag caagcaggaa cttaaagaag ggtgtatggt agaaatagaa
    10201 ggacaattca gatcctacaa caactatgcc agggagggca acaaactgtt gctaactata
    10261 tttgccaggg aaattaattt tttggaagac gacggcaaga ttaaaaaccc aaatcaggtt
    10321 tatttaaatg gatacatatg taaaaagcct atatatagaa tgacaccttt tggcagggaa
    10381 attactgatg ttttattagc agtcaacaga ccctacaaca aatccgatta tataccatgt
    10441 attgcatggg gtagaaatgc aagatattca gaaagccttt ctgtaggaga taatattaag
    10501 atttggggga gaattcaaag ccgtgagtat cagaaaaagt atgattcggg agaaatcata
    10561 acaagaacgg cttatgaaat ttcaatatca aaaatggaag tatgcaataa ggaacaaact
    10621 gattaccagg agcttccata cacggaaaat gaggaaaccg atgatcatac atgatggcag
    10681 ctgtaattaa atttttgcag atacacttgg ctggaatacc gtaaaaagat attttatttg
    10741 ccaaatagaa atatggctga taaagtatct ttttattttg tttaatatgt acaccactaa
    10801 taaagtaata aagtttcatg cttgaaataa cattcaaaat atattattat ataagtaaca
    10861 gggaggggtg gtgtgtatga atgattaaag ttatttatgg agaaaagggt acgggaaaaa
    10921 caagaattct cgttgaaagt tccaatgtaa tggcagaaag aaattatgga catgtagtat
    10981 ttatagaaga tagcaaccag tctatgtata aacttataca taaggtaaga tttataaata
    11041 tatcggagtt tccgatatat ggcgaggatg gatttattgg ctttatttgt ggaattatat
    11101 ctcagaatta tgatacaaaa ggtattttta ttgatggctt gacctatata ttaaaaaaag
    11161 atgttaacga tttggaacca tttttttcaa agttgaaagt aatcgcctct aaatttaatg
    11221 ttgagtttat attaagtgta aatggcaatg cggaaaatat acccggcttt ttgaaagaat
    11281 atattttata gtatatagta taatggatag aggtttttat ttatatttaa aaaacatgca
    11341 taaagataag gctttatgca tgtttttttg aaagaaatga attttgaaag attgaatttg
    11401 gaaggactga aaatggataa tattgcttca gttattataa gtaacagtac aagagaattt
    11461 gataaggaat accattacct gattcctgac aatatgaaag ggataattaa atcgggtatg
    11521 agagttatag taccttttgg aaaggcaaat actctgaggg aggcatttgt ttttagaact
    11581 tttgaaagta atgaagcaat ggggcttacc ggacttaagt acataaaaaa attgatagat
    11641 aaaactcctg ctcttgggga gaacatgatt aacatggcca tatggatgaa agaacgttat
    11701 atatgtacat actatgatgc aataaaatgt atgcttccat ctggtacaaa ggtaaaaacc
    11761 tacaagatag taaagcttga aggctccagt aatactgatg aaatacaggt aaataataat
    11821 aaaatattaa cagatgaaaa tatatataaa aatatagata aaaatgaaga attaataatt
    11881 aaaactcttg ctcaaagtgg taaccaatgt gaaatggaag agttaaaagg cataattaat
    11941 attaaaaaca tttctagata tataaaaaag cttagtgata aaggcctgat atgcatacaa
    12001 gagggatata ctgcgagggt aagggagaaa ttcgtaaaag ctgcatatgc tgccattcct
    12061 gctgaagaga tagctgaaat aattgatagc aacagaatta aaagaattca gcagataaaa
    12121 gtacttgaaa tactaatgga aaacgaatat gtggcagtac aggatattgt aagatttgca
    12181 ggggtatccc agggggtcct tgatactctc aaaaagtatg gatacataga ttttagggaa
    12241 attgaaatca aaagggatcc ctttgcaaat agggagtata aaagaacaga acctttaaaa
    12301 cctacccttg agcaggaagc agcattaaaa atacttaaag aacaaaagga caggcagttg
    12361 tttgctgagg atctccttta tggtgttaca ggcagtggga aaactgaagt atatttgcag
    12421 ctgatacaat atgcactgga taaaggagag gaagccatag ttcttgtacc tgaaatatca
    12481 ttaactcccc agatggtaga aaggtttaaa ggcagatttg gaaatcaggt ggctgttctt
    12541 cacagccggc tctccctggg tgaaaggtat gaccagtgga gacttataaa ggatggaaaa
    12601 ataaaagttg taattggtgc cagatctgcg atttttgcgc cccttgcaaa tattggatta
    12661 attattattg atgaagagca tgaagcaagt tacaaatcgg aaataactcc caaataccat
    12721 gcggctgata tagccagata tatatgcaaa catcggaatg cggttttatt attaggttct
    12781 gcaacaccat cagtggaaac ctattataaa gctgtaaatg gtgaaatagg acttatcaat
    12841 atggaagaaa gagctaacaa tatgacaatg cctcatgttg agattattga tatgagagag
    12901 gagctaaacc agggaaaccg ttcagttttc agtagaaaac tagctgtaga aattgataag
    12961 aatattgccg gcgcagagca aaccattatt cttcttaaca gaagaggata ttcccccttt
    13021 gtactttgca gaagctgcgg ttatgtggca aaatgcatca attgcagtat taccatgact
    13081 taccatgctg taggagaaag attaacctgt cattattgcg ggtacactat taaagtaccg
    13141 gaaaaatgtc ctaaatgcgg gagcggctat attagacatt tcggtaccgg aacacagaag
    13201 cttgaagaag aaactaaaaa acaattccca ggtgcttctg taataagaat ggatatggat
    13261 acaacaggat ataagaattc acatgaagag atacttggaa aattcagaga taaaaatatt
    13321 aatattatgg ttggaaccca gatggtggcc aagggacacg attttcccaa tgtaactcta
    13381 gtaggtgtgc tggcagcaga cagtatattg aacattggag actacagggc atctgagaga
    13441 acattccagc tgctgaccca ggtggcagga agagcgggca ggggggaact gcccggaagg
    13501 gttgtaatac aagcgtataa tacagatgat tttagcatac ttgctgcttc cagacatgat
    13561 tttatttcgt tttatagtaa ggaaattaaa atacgcaggg aaatgagtta tcctccattt
    13621 acaaatatag ctacagctgt tttaagtgga aggtatgaca aaaatgtatt tgaatgctca
    13681 aagagatttg aggacatatt aaacagctta tttatatatt atgaaattca taaggatagt
    13741 tatgaaattc taggatctat gaggccccca ataggaaaaa taaaaaacaa gttcagatgg
    13801 agaatagtta taaagagcac ggatattaat actttggttc gagttttaag cggggcttct
    13861 gatgaatatt ataaattaaa gtataaagaa aaggctcagt taagtataga tataaatcct
    13921 gttagtatgt tataataaat acgatcaagg gagtaaaacc gaggcggttt tatccttatg
    13981 tatttattga aacggtgtga agcaatccga ttggcggatt gcacctatgt tataaataaa
    14041 ggggattcag ttggaggatg aatatggcta ttaggaaaat tagaaaagat ggggatgaag
    14101 tcctgaggaa aaagtcaaaa aaagtagagg tcattaatga taaaattata tccttactta
    14161 aagatatggc tgaaaccatg tatgaaagtg atggagtagg actggctgca cctcaggttg
    14221 gaattttaaa aagggttgtt gtaatagatg ttaatgatga tagaggcctt atagaattaa
    14281 taaacccgga aatcatattt agtgagggcg agcagaatga atcagaggga tgcctgagcg
    14341 taccgggtgt atatggacag gtgaaacgtc ctgccagggt tattgttgaa gcattaaatt
    14401 caaagggcga aaaaataacc gttgagggtt caaagctgct tgcagtagct ttgtgtcacg
    14461 aaatagacca tcttgacggg atgcttttta cagataaagt aattaattac acccataaat
    14521 agttaacttt aatatagatc ggagtgaaga attgttgaaa attgttttca tggggactcc
    14581 tgaatttgct cttcccagcc ttgatatgct tgtgagggaa ggccatgaaa tagctgctgt
    14641 tgtaacacaa ccggacaaac caaaaggccg gggccaaaaa ctctgttgtc cccctgtaaa
    14701 agaatatgca gataaaaatg gaataattgt tttgcagcct tataaggtta aaacacctga
    14761 tttcatatac tctataaaag cattggagcc tgatttgctt gttactgcag catatggtaa
    14821 aattttacca cttgaaatcc tcaatatacc aaaatatgga tgcattaatg ttcatgcatc
    14881 tcttttacct gaatataggg gagcggcacc tattaattgg gcaataataa atggagagct
    14941 aaagacaggt gtaaccacga tgcttacaga tataggaatg gatacaggtg atattttgtt
    15001 aaaaaaggaa atgaacatac ctgatgatat gattgcagga gaattgcatg ataaattggc
    15061 agtttttggt gctgaatgcc ttaaagaaac acttgcaaaa cttaaagata acacattgta
    15121 taggatacct cagcaacatg aaaaggcctc atatgctcca atgcttaaaa aggatgttgg
    15181 acttattagc tggggaaagc cctcccggga aattcacaat cttgtcaggg gtactaatcc
    15241 ctggcctgtg gcatatactt tttacaaaga aaagcgagta aaaatatgga agacccagac
    15301 cgaattaagg cttgaaaatg aaaatgatgc atgttatgat gcataccagc caggtactat
    15361 ttgcaaaaga gacaaagatg gtatttttgt aaaatgtgga gaaggattta taaaaatatt
    15421 ggagctgcag tttgatggat gcaggaagtt atgttccgat gagtgctggc ataactttga
    15481 gtctggtgag tccctggggt agagcaaaca gggacggttc ttagaaaaga aggaaaaaag
    15541 gagatgaatt tttatgggtt atttctatgg cttcgacaaa tattacttaa tgctggtttt
    15601 acctgcattt atattttctt tatatgcaca gtttagagtt aagagcacat tcagtaaata
    15661 ccaacgtcta tccaacagga gtggttatac aggtgctgat gttgctcgtg ccatattgga
    15721 taagaacgga ctgggaaatg tgtcggtaca aatggtacca gggcagttga cagatcatta
    15781 tgaccccagg acaaatgtag tacggctttc acaatcagta tacgggagca cttctgtagc
    15841 agctttggga gtagctgcac atgaaacggg tcatgcaatt cagcataaag ttggatacag
    15901 gcctcttgtt ttaagaacca cccttgttcc aattgcaaat ataggctcta tggccggtcc
    15961 ataccttgca atattcggat tgctttttga ctttttgcag ttggcatata taggaataat
    16021 attatatgca ggggcggtag ctttttacct tataaccctt cccgttgaat ttaatgcaag
    16081 taccagagct atagaagctt tggaaactac aggtatatta acatgggatg agtcagaacc
    16141 tgcaaagaaa gtcttaaaag cagcagcaat gacttatgta gcatccgccg ctgtagcaat
    16201 tgccagcttg cttcgtttgc tgcttcttac aggaaacaga agaagaaatg attaatgtaa
    16261 tattagatgg attgcaggtg taataatgcc agttgattta cctagggaaa tagcattgaa
    16321 aatactatat gaaataaatg aaaaaggggc ttattcaaat attgctataa ataagcatgt
    16381 agaaaacccg ggtttgaggg atattgacaa ggtttttata acaggacttg tatatggtac
    16441 tataaaatgg aggttgtcca ttgattttgt tatagggcag ttttcaaagc ttcctatgaa
    16501 aaaattgtcc ccatggatat tgaatatttt aagattaggc atatatcaga tactatatac
    16561 agataagata cctgtgtcag cagcctgtaa tgaaagcgta agccttgctt taaaatacgg
    16621 acataaagca tcaggcggtt ttgttaatgg aatcctcagg aatgttgcaa gaaacaagga
    16681 taatattgtg taccctgata agaatgcaga tttaataaga tatttatcta ttaagtattc
    16741 ccatcaggaa tggatgatta aaaaatgggc aaagctttat ggtgaggagt ttactgaaaa
    16801 cctgctacag agcaataatg aaaacccggt attcagcata gcggtaaata cattaaaaat
    16861 atcccgggaa ggcataagac aaaagcttca tgaagaaggt atagactgca ttgaaggaaa
    16921 atattcggat aatgctctta tactggagaa tccggcagga atagtccatt cgtacctgca
    16981 taaagaaggg tactttcaga ttcaggatga agcatccatg ttggtttcaa aagtcctgga
    17041 ccctgcgccg ggggagctcg ttatcgatgt ctgtgccgct cctggaggta aaacagctaa
    17101 tattgcacaa atgatgaata attgtggaac agtagtagca agagatatac atcctcacaa
    17161 gataaaatta attgaagaag cttcaaaaag attggggttg aatatagtta aaacacagtt
    17221 atttaatgca gaagaaatag acccttcatg caaaagcagg gcggatcggg tattggtgga
    17281 tgcaccttgt tcaggactgg gtattataag gagaaaaccg gatataaaat ggacaaggac
    17341 acctgaagat atgaaaacta ttcctcctct tcaatttaaa atattatgta ctgcagcgga
    17401 atatgtaaaa ccgggaggag tgctggtata tagtacctgt actattatgc ctgatgaaaa
    17461 tattgatgtt gtaagtaaat tccttgaatt aaatagtgat tttaaaatgg ttggcttcga
    17521 aact
//
LOCUS       DAOSDD010000078        17501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1317_length_17501_cov_4.807750, whole genome shotgun sequence.
ACCESSION   DAOSDD010000078 DAOSDD010000000
VERSION     DAOSDD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17501
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1317_length_17501_cov_4.807750"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..508)
                     /locus_tag="PK733_08660"
     CDS             complement(368..508)
                     /locus_tag="PK733_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10649.1"
                     /translation="MADSRGGCGLGCGFGNEWIWIIIIIIIIFCCCPFSFGGFGCGCD
                     HK"
     gene            858..2915
                     /locus_tag="PK733_08665"
     CDS             858..2915
                     /locus_tag="PK733_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HOJ10650.1"
                     /translation="MKKTIGIFAHVDAGKTTFSEQLLYYTGSIRNIGRVDYKTSCMDT
                     NEIEQKRGITIFADQGIFTFEGDTYYLIDTPGHVDFSSETERAICALDYAIMLISGSS
                     GVQAHTTTLFRLLKSYRIPTFFFINKTDIDGFNMKSIIEDIKNKLTDDILYFNSIKDI
                     LKTGYKEAEATPEAAPNATPEAAPDAERTAELVAEFAAERDEEFMEAYLQGDYTVDSL
                     QEAIIGLIRKQECFPVMSGSALKGSGIATFLETFSKLSSTFYEGAAERKPSFAGNVYK
                     IRHDEKGNRLTFIKALEGKLKVKDEFTFEKSGGTYVEKVNEIRIYNGKKYDNKNVVSA
                     GEVFAVTGLKTPVCGTTLETGKITVRAGKNYYLVPALQSKINILDETDITTCIEKLRI
                     LESEDPMLSVSFQKESGQILVSVMGKIQLEVLEQLIATRFGISITFEKPQVQYRETIG
                     ASTVGYGHFEPLRHYAEVHLRLEPNPRGDGIIFASECHVDTLTLNYQRSIEIHVFEKP
                     HKGVLTGSPITDVRIVLQNGRAHLKHTEGGDFREAVYRAIRQGLEKAESILLEPFYRF
                     EIYAEAAYLGRIMSDIERMRGTYESPIQTGENVYIRGRGPVETFMEYSVDLVAFTKGN
                     GSISFMFDGYDVCQNADEVIKKIRYDKNKDVENTSSSVFCAKGTSFIIPWDKAENYMH
                     IPL"
     gene            3189..4514
                     /gene="uvsE"
                     /locus_tag="PK733_08670"
     CDS             3189..4514
                     /gene="uvsE"
                     /locus_tag="PK733_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HOJ10651.1"
                     /translation="MNTRIGYACLTVGVQHTGQKSCMLKNASEEKLLELIHFNLNSLE
                     NIIDYNIKNNIKLFRISSDLIPFGSSSVNNLSWWNIFASQFTKIGEKIKNNGMRVSMH
                     PGQYTVLNSPDAGVVERAIKDLNYHARVLDSLGMGAEHKIILHMGGVYNDKKQAIKRF
                     IANYYHLDDSVKQRLVLENDDKLYNICDVLEVGTILNVPVIFDNLHNKINSCDNQKDD
                     FYWISECRKTWNMKDGKQKIHYSQQDPAKKPGSHSNSICINEFMDFFKSLSAKDTKDT
                     KDTKDTKDMKDTKDMKDIDIMLEVKDKNLSAIKCINCISVNKGIKALELEWARYKYKI
                     LENSPSGYVEIRELLQNKNSYPAVPFYNLIEDALQKECTIGNSINGALHIWGYFKNTA
                     SEKEKNNFIKSIDVYKQGTTSIKTVKNSLWKMAVKYQQLYLLDSYYFVL"
     gene            4803..5270
                     /locus_tag="PK733_08675"
     CDS             4803..5270
                     /locus_tag="PK733_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10652.1"
                     /translation="MKEYKILFGVGTFIAVNLIIALFHFLSDLKLTADGVIGTISLTK
                     VIFSMLFIFGLFVLAFIYGYQCNISGLIGLAMVFVLPFIGLLGGVFYLLIFWSAAPYL
                     PIFYLLEISTGSDYYIGFSVLATITAIAWFLGKFIKIKRAKYTKSSNLQNDKS"
     gene            5459..6208
                     /locus_tag="PK733_08680"
     CDS             5459..6208
                     /locus_tag="PK733_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10653.1"
                     /translation="MELVNINQYIRSVELCKDKIPSFSSYPFSLPAVRNMNELKLHPN
                     VTFIVGENGTGKSTILEAIAVAYGFNPEGGTKNFNFSSMNTHSELYNFVKLTKGVKRP
                     KDGFSLRAESLYNVATNIDELDKEGWAPKIIDSYGGLSLHEQSHGESFMAIFLNKFHG
                     KGIYILDEPEAALSPSRQMTIIARMHELVGQESQFIIATHSPILMAYPNSIIYEIKDN
                     YKIVKYEETEHYQITRAFINNTQKMLDVLIG"
     gene            6367..7071
                     /locus_tag="PK733_08685"
     CDS             6367..7071
                     /locus_tag="PK733_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10654.1"
                     /translation="MKILFVEDDKIIASGLCYSLTQEGYFVTHCLNVESAKTLINENS
                     YDLAILDLSLPDGSGYDICKMIKAKKDIPVIFLTAIDDEVNVVMGLDMGADDYVTKPF
                     RIRELLSRIKSVLRRYDKTGSSKTLLEFGDVKIDTTQAKVYKENQEIMLTSLEYRLLL
                     IFANNEGQVLSRNQLLEGLWDFAGDFVNDNTLTVYIKRLREKIEDDSQEPKIIKTVRG
                     VGYMGHGNKECGHKGR"
     gene            7150..8151
                     /locus_tag="PK733_08690"
     CDS             7150..8151
                     /locus_tag="PK733_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ10655.1"
                     /translation="MLRNKEIRIYIVASVLVAATGTAACFIIDIIAGIIVFSALVLMF
                     AAFLLFTIWRYKQIEELSQYLKRIANGEYSLDVRDNNEGELSILKSEIYKVTVTLREQ
                     AELLKKDKLFLADSISDISHQLKTPITSMFVMTELICDENLPQDKRVEFTQNIRSQLE
                     RLQWLVASLLKLSKIDAGTVEFRKENVNVKELIKKAVEHLLIPMEIKEQTLEISGDER
                     VHFIGDLNWSAEAVTNIVKNCIEHTHCGGIIGINYDQTPIYTIIKISDNGVGIHKDDL
                     PNIFKRFFKGKNAHSDSIGIGLAMSKSILQKQGGIIEVASEKDKGTQFVIKIYKNIV"
     gene            8625..9308
                     /locus_tag="PK733_08695"
     CDS             8625..9308
                     /locus_tag="PK733_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10656.1"
                     /translation="MEILRVENLSKIYGKGETAVKALNNVSFSVNKGEFVAIIGPSGS
                     GKSTLLHILGGVDTPTSGRVFVENTDVYALDETKLAIFRRRQIGLIYQFYNLIPVLNV
                     EENTTLPLLLDGRKVNKNQFNEIISTLGLTDRLNYLPNQLSGGQQQRVSIGRALINNP
                     ALVLADEPTGNLDSKNSADIIELLKLSNKRYNQTLIIITHDERIALQADRIISIEDGQ
                     IAKDEVIHA"
     gene            9305..12034
                     /locus_tag="PK733_08700"
     CDS             9305..12034
                     /locus_tag="PK733_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ10657.1"
                     /translation="MNIMNALTLRHLKLNKKRTVVTIIGVILSVSMITAVPTIMTSLL
                     SMMQRSVIADTGNWHVLYADILGQNVNIVMNDKNTDTAALSKDVGYAVLRGSKNKNKP
                     YLFIKSYDEQGFINFGLKLIEGRFPQSSNEIVISSHVAENGGVEYKVGDVLKLDIGQR
                     YMVEDEKISTLGQTSGFKEASDDNKGEKFAEQFTKEYTVTGIISRPGFEPYWAPGYTV
                     ISYLEKNELTPNDTVNVSVAWKSINKKANEHANKLFESINTSGSSSITYNRQLLRYYG
                     IIGEDLLTMLYTLAAIVTLLIITGSVALIYNSFAISISERSRHLGMLASVGATKKQKS
                     RSVFFEGFVVGIIGIPLGVFFGTLGIGVTFVLVQPLIEGLNNTGVKLTLVTSPGTIVV
                     AVLLSLLTIFVSAYVPARRASKVSPVDALRQSQDIKLTSRAVKTSRLTRKIFGFEAEL
                     GLKNLKRNSRRYKATVFSLVISIVLFLSVSSLSLLTRKSVDIVSGGYPYDVRVFVVSS
                     ATAQEKKDFYTAITKMAYADKSVIEQTMNAVFTADGKLIPDQIKEIIEPNYKDIEPNY
                     KDIEPNYKENKSETNTNTFDMEAEIKSIDEAALERYAKEIGVDPARLKDTENPCGVLV
                     NTVTIKVGNKYSRMKHFNIEQGQKLKFIHHIYDGKSSGEPQKPGELGGPGEPEEYGTI
                     LEIAALADSTPIGETTFTDPLRAVFIVSEEVYAHMQAKLPEESHNTRVLLLIKSSNPS
                     KLVKGIEEYQKQTSIASVDIFDVAALDKANRQFETFIFVFFYGFVALITAICAANIFN
                     TISTSISLRKREFAMLKSVGMTPEGFNKMINYESLFYGIKALLYGLPISFGIMYLIYR
                     VLSNSFGFAFAIPWSSVLAAVIVVFALVGSTMLYASSKIKKENIIDALKTENI"
     gene            complement(12202..12981)
                     /locus_tag="PK733_08705"
     CDS             complement(12202..12981)
                     /locus_tag="PK733_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ10658.1"
                     /translation="MEYTVQKLGKIAGISTRTLRYYDEIGILKPARINSSGYRIYGQS
                     EVDRLQQILFYRELGVSLNEIKDIVTVPSFDKHKALQEHREKLLEKRKQLDLLIANVD
                     KTIALAEGRVKMTDTEKFEGFKQKMIDDNERKYGIEAREKYGDQAFNKSIQKIKGMSE
                     EQYEESVRLGNEVIETLHTAFKTGNPAGDLAQKAADLHRQWLSFFWDSYTKEAHAGIA
                     QMYVDDDRFTAFYDKNQPGAAKFLRDAILIYTGMKHGDSPA"
     gene            13234..13539
                     /locus_tag="PK733_08710"
     CDS             13234..13539
                     /locus_tag="PK733_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOJ10659.1"
                     /translation="MYPNIYHNNRVGIIPYVLQPNDNLWLLAQRYNTTVYDIAAANPG
                     IDFNFLQAGQLIYIRPGFGQYSTVSNIGTPETPVCGINKKELNLINYLRMLWEEHVT"
     gene            13573..14019
                     /locus_tag="PK733_08715"
     CDS             13573..14019
                     /locus_tag="PK733_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ10660.1"
                     /translation="MPDVDLVTSRLLQNPKDFEEALKPFYGNKIASSFADLFTNHLVI
                     AAQLVKAAKAGDSNAAKDAEKRWYSNADKIAAFLAGINPYWSQDKWKRMLYEHLAMTK
                     SEAVYMLTKNYGAGITEYDKIEKQALKMADVMAYGIIKQFPDIFAY"
     gene            14253..15410
                     /locus_tag="PK733_08720"
     CDS             14253..15410
                     /locus_tag="PK733_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOJ10661.1"
                     /translation="MDYLWVLEPEAEYGTIGDKLDTAGNEHDTVWNKHDKGYKYLLSV
                     KRVFMQLLRSFVDESWVGSIDEDSIEYINKSFILQDFKGKEADIVYKLKIDGREVFFY
                     ILLELQSTVDFQMPYRLLQYMLEIWRTILKDTKKAIEKQKEASKDNTENRSEGKIGDE
                     VERKIEVETKNEVENDINDIEKNKEMEIGKDGKDFRLPVIIPCILYNGEYNWTAPRSS
                     NDILIANGLFDDYIIDFNYILFDVVRYDEEELLRLGNLVGAVFYIDQKQQYDEIIERL
                     KNLVDQLKDLREEDFELFKIWLKNVVTRGMPKEEAKEIEKIIESNKEADSMVYNMEIA
                     IRNKMKEVELKGKQEGKLDVAKNLFDAGMDIEQIGIVTNIPVDELQKLIVK"
     gene            complement(15517..16287)
                     /gene="fetB"
                     /locus_tag="PK733_08725"
     CDS             complement(15517..16287)
                     /gene="fetB"
                     /locus_tag="PK733_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HOJ10662.1"
                     /translation="MNGSNTINITSLLIVSTLVMVSLFFSYWQKLKLEKETIISVVRA
                     VIQLIAVGYILEYVFGYKNPVFTTLLLLFMTFNAAYNAAKRGKAIKNGLLISFLSILT
                     GTLVTLTILILSKAVQYEPYQIIPVSGMIISNSMVALGLCYKQMTSDFKDKREEVETK
                     LSLGADILPSSIEIIRNSIKTGMLPTIDSAKTLGIVSLPGMMTGLILAGTPPLEAIKY
                     QIMVTFMLLSTTSISSFIACYLSYRGFFNSRKQLIYMK"
     gene            complement(16325..16981)
                     /locus_tag="PK733_08730"
     CDS             complement(16325..16981)
                     /locus_tag="PK733_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ10663.1"
                     /translation="MSLLQFENVYFDNDGKTILKNICVDIESGDFISVVGPSGSGKST
                     FFKLCSHLISPTRGNITFKDKNITEYSPTELRKSIVYCFQTPYLFGNTVLDNINFPFS
                     IRNMKPDIKKINELFSIFHMSADFLNEDVKSLSGGEKQRLALIRSLLFKPEILLLDEI
                     TSALDVNNTEIVENVIVSLNREGMTVLWITHNPEQSRKHANKLLTIEAGEIKSLDVLV
                     "
     gene            17386..17487
                     /locus_tag="PK733_08735"
                     /pseudo
     CDS             17386..17487
                     /locus_tag="PK733_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
BASE COUNT         5977 a         2812 c         3443 g         5269 t
ORIGIN      
        1 actgtacgga aacttgtgta caaagtagag agttatattg atattttgtt ttaagataac
       61 aggcaagtgt aacagacaag tgtaacagac aattgtaaca ggtaagtggg gacggttctt
      121 gacttgcgag tatttacctt taaattggca ggtagagaac cgcccgagaa ccgccaactt
      181 ttgtctgttg aggacttttc ccatttgcta tttacatttt ctactctaac ctccatttat
      241 ccagcccctt atgccatgca tttgccacat gatacacttg taagtcaaga accgtctcca
      301 cttgtttccc tcctaagaac cgtcctcatt tgctatattg gttaattaac accgtcaatt
      361 aacaccatca tttatggtcg catccacatc caaatccacc aaaggaaaat gggcagcaac
      421 agaatataat tatgattatt ataataatcc atatccattc attgccaaac ccgcagccca
      481 atccacatcc tcctcttgaa tcagccattt aaatcactcc tttcttattt agttagggta
      541 cagctacatg gttgaatatt ggtcctgcac agtttcagaa gtgcaataaa attacaagta
      601 taagggacaa aatcacaaat cgtaaaaacc gccagaagca tgtctattat ctgctgtatt
      661 tacctctctt aatgttgtat catagtaata ttttatgtta acatatatct aattgttaca
      721 ttatctcatt taaatcccga atcaaatcca gtaaatgcac ccctgtaaat gctatacatg
      781 gcaagatgca tatttttatg ttaaactatt tattgatgaa aataaaacag gaaccagcaa
      841 aatgaggaat taaatttatg aagaaaacta taggaatttt tgcacacgta gatgcaggaa
      901 agactacatt ttcagagcag ctgctttatt atacaggaag catccggaat attggaagag
      961 tagactataa gacttcctgc atggatacaa atgagataga acaaaaaaga ggcattacta
     1021 tatttgcaga tcaaggcata tttacctttg agggagatac ctattatctt atagataccc
     1081 ctgggcacgt ggatttctcg tcagaaacag aacgtgctat ttgcgccctg gattatgcaa
     1141 ttatgttaat tagcggaagc tcaggagtgc aggctcatac aacaaccttg ttccgacttt
     1201 tgaagtctta tagaattccg acatttttct ttattaataa aactgatata gatggcttca
     1261 atatgaaatc cataatagaa gatatcaaaa ataagctgac tgatgatatc ttgtatttta
     1321 actctattaa ggatatttta aaaacaggct ataaggaagc agaagcaaca cctgaagcag
     1381 cacctaatgc aacacctgaa gcagcacctg atgcagaacg tacagcagag cttgttgcgg
     1441 aatttgcagc ggaacgagat gaagaattca tggaagctta tctccaagga gattacactg
     1501 tagattcttt acaagaggct ataattggtc taataagaaa acaggagtgc ttccctgtta
     1561 tgagcggatc cgccttaaaa gggagcggaa ttgctacctt cttggaaacg ttttccaagc
     1621 tttcatcaac tttctacgaa ggggcggctg agagaaaacc ttcctttgcg gggaatgtct
     1681 ataagatacg ccatgatgag aaaggcaatc ggcttacatt tataaaagcc ctggaaggta
     1741 aattgaaggt taaggatgaa tttacttttg agaaatccgg agggacttat gtagaaaaag
     1801 tcaatgagat ccgcatttat aatggtaaaa agtatgataa taaaaatgta gtttctgcag
     1861 gagaagtatt cgctgtaaca gggctaaaga cacctgtttg cgggaccact ttagaaacgg
     1921 gaaaaataac cgtcagggca ggcaagaact attacttggt tcctgctctt cagtcaaaga
     1981 taaacattct ggacgaaaca gacattacca catgtataga aaagcttcga atattagaat
     2041 cagaggatcc aatgttatct gtatctttcc agaaagaatc aggacaaata ttggtaagtg
     2101 taatgggcaa aatccaactt gaagtattgg aacaattaat tgcaacaagg ttcggcatat
     2161 ccattacttt tgaaaaacct caggtgcaat accgggaaac aataggggca tctacagttg
     2221 gatatggaca ttttgaacca cttcgccatt atgcagaggt ccatttgagg cttgagccaa
     2281 atccccgggg agatggtatt atatttgcca gcgaatgtca tgtagacacc ttgactttaa
     2341 actatcagcg atcaatagag atccatgtct ttgagaaacc tcacaagggt gtattgaccg
     2401 gttcaccaat aacagatgtc aggattgtac tgcaaaatgg acgtgcccat ctaaaacaca
     2461 ccgaaggcgg cgactttagg gaagctgttt accgggcaat caggcaaggt cttgagaaag
     2521 cggaatccat attattagaa ccgttttata gatttgaaat ctatgcagaa gcagcctatc
     2581 ttggacggat aatgtcagat atagaaagaa tgagaggaac ttatgaatca cctatacaga
     2641 caggtgaaaa tgtatacatt cggggaagag gacctgtaga gacattcatg gaatacagtg
     2701 ttgacctggt tgcctttaca aaaggaaatg gcagtattag tttcatgttt gatggttatg
     2761 atgtgtgcca gaatgcggat gaagttatta aaaaaattag atatgataag aataaagatg
     2821 tggaaaatac atcgtcgtcg gttttctgtg ctaaagggac ttcatttatt attccctggg
     2881 acaaggcaga aaattatatg catataccac tataatttac actaattggg gagtgtattt
     2941 aagttttgaa gaaatatatt ccaaagggta ttgctatggt acttagagcc ttaaattcag
     3001 ctacgtgatt gagttcccgc ttgtagaggt agacgacttt cagatgattt gattaaagga
     3061 ggttatcgat gaacatgcca gaaaatatgc aagtgaatat gctggtgaac atgtcagaaa
     3121 atatgccagc gaatatgcca gtgaacatgc cagcgaatat gccaatgaac atgccagcga
     3181 atatgccaat gaacacaaga ataggatatg catgcctgac tgttggagtt caacataccg
     3241 gacagaaaag ctgcatgctt aaaaatgcaa gcgaagaaaa attgttggaa ttaattcact
     3301 tcaatttgaa ttctcttgaa aatataattg attataacat taagaataac ataaagcttt
     3361 tcaggattag ctctgactta attccattcg gttcgagttc tgtgaataat ctttcgtggt
     3421 ggaatatatt tgcatctcaa tttacaaaaa ttggagaaaa aatcaaaaat aatggtatga
     3481 gggtttctat gcaccctggg caatatactg ttttgaactc tcctgatgca ggagttgtag
     3541 aaagagccat taaggattta aactatcatg cccgtgtgtt ggacagcctt ggtatgggag
     3601 cagaacataa aattatactg catatgggag gggtatataa cgataaaaag caggccatca
     3661 agaggtttat agcaaattat tatcaccttg atgattctgt gaaacaaagg cttgtcctgg
     3721 aaaatgacga taaattatat aatatatgcg atgtattgga ggtaggcact atactgaatg
     3781 tgccggttat tttcgataac ctccacaaca aaataaattc ttgtgataat caaaaggatg
     3841 atttctactg gatcagtgaa tgcagaaaaa catggaatat gaaagatggc aaacagaaaa
     3901 ttcattattc ccagcaggat cccgcaaaga aacccggatc acattctaac agcatatgta
     3961 tcaatgaatt tatggatttt tttaaaagtc tcagcgcaaa agacacaaaa gacacaaaag
     4021 acacaaaaga cacaaaagac atgaaagaca ccaaagacat gaaagacatt gatataatgc
     4081 ttgaagtaaa agataaaaac ctttcagcca taaaatgcat taactgcatt tccgtaaaca
     4141 aagggatcaa agcacttgag cttgaatggg ccaggtataa atataagatt cttgagaatt
     4201 caccatcagg ttatgtagaa atacgtgaat tacttcaaaa taaaaacagt taccctgccg
     4261 ttccttttta taatctaata gaagatgctt tacagaaaga atgcacgatt ggaaattcca
     4321 taaatggagc actgcacatt tggggatatt ttaaaaatac tgcatcagaa aaagaaaaga
     4381 acaactttat aaagagtatt gatgtatata aacaggggac tacatccata aagacagtta
     4441 aaaacagctt gtggaaaatg gctgttaaat accaacaatt gtaccttttg gattcttatt
     4501 attttgtgct ttgaaaaata atctatgtga ggaaaaataa ttctaataag gtattcaata
     4561 ttaagatgat caggtaaaag atcataaggt gaaaaatgta aatatgagat tgacttttta
     4621 attcatgtag tataaattag ggctaaaggg tataggagta tcgggatgag gctaaaatac
     4681 caaacctcgg aatatacaaa aattgatgct cttcggcttt tcgagaacat gctttattag
     4741 ctgtaggata cacaggaggt acatagtagg gtttaaatta taggaggtat agaaggtaca
     4801 taatgaagga atataaaatt ctattcggag taggcacatt tattgctgta aacttgataa
     4861 tagcgttatt tcatttcctt tcagatttaa aattaacagc tgatggcgtt attggaacaa
     4921 tttcccttac gaaagttata ttttccatgc tttttatttt tgggctgttt gtccttgctt
     4981 ttatttatgg ataccaatgt aatataagtg gtttaatagg gttagctatg gtatttgtgc
     5041 tcccatttat tggattgtta ggaggcgttt tttatttgct gatattctgg tcagcagcac
     5101 cctatttgcc tatattttac cttttggaga ttagtaccgg gtccgattac tatattgggt
     5161 tctcggtatt agctacaata actgctatag cttggttcct gggaaagttt ataaaaataa
     5221 agcgtgcaaa atatacaaag tcatcgaatt tgcaaaatga taaatcataa gctaaatctg
     5281 aagctaaatg tttatgtatt cgcaaatttg gcaatattta tatttatgtt ttaggttaag
     5341 taaaggattt acgatagatt tactatagat ttactataga tttacggata gatttacgga
     5401 tagatttacg gatagattta ttgatagatt tatggatggc attcacggag aataaagaat
     5461 ggaattagta aatattaacc aatatataag aagtgtagaa ttatgtaaag ataaaatacc
     5521 gtccttttcc agttatcctt tttctcttcc tgctgttcgc aatatgaatg aattaaagtt
     5581 acatccaaat gttacgttta tagtaggtga aaatggaacg gggaaatcaa caattttaga
     5641 agctatcgct gtagcatatg gttttaatcc tgaaggggga acaaagaatt ttaacttctc
     5701 ctctatgaac acacattcag agctatacaa ttttgttaag cttactaaag gagttaaaag
     5761 acctaaagat ggtttttccc ttcgggcgga aagtttatac aatgttgcta ctaatattga
     5821 tgaacttgac aaagaaggtt gggcacccaa aataattgat tcatacggcg gactctcatt
     5881 acatgagcaa tctcatggtg agtcgttcat ggcaattttt ttgaataaat ttcatggaaa
     5941 aggtatatat attttagacg agcctgaggc ggctctttca ccctcaaggc aaatgacaat
     6001 aattgcgagg atgcatgagc tggtcggaca ggaatcccaa tttataatag ctacccattc
     6061 cccaatatta atggcatatc ctaattccat aatatatgaa ataaaggata attataaaat
     6121 tgttaagtat gaggaaacag agcattatca gatcacgagg gctttcatca ataacaccca
     6181 aaaaatgctg gatgttttaa taggctgaat tttaacctgc ccccgtcgca atgtatagct
     6241 gcaaacaaat aaatgtgcgg atgtgcattg cagcgaatca agctgtgccg acggctatag
     6301 tgacggaata gagatttata tcagcaaaat tctggttaat aacgatgaaa tggagattta
     6361 tataatatga aaatactgtt tgttgaagac gataaaataa ttgcatcggg gctttgctac
     6421 tcactcaccc aggagggata ttttgttacc cactgcttga atgtggagtc ggcaaaaact
     6481 cttataaatg agaatagtta tgatttggca atacttgatt tatcccttcc tgatggaagt
     6541 ggttatgata tatgtaaaat gattaaggcc aaaaaagata tacctgttat atttctgaca
     6601 gcaatagatg atgaggttaa tgtagttatg ggcctggata tgggggcaga tgactatgta
     6661 acaaagccct tccgtataag agagcttctt tctcgcatta aatctgtact tcgcagatat
     6721 gacaagacag gatcatcgaa aactttgctg gagtttggtg atgtaaaaat tgatactaca
     6781 caagcaaaag tgtataagga aaaccaggaa attatgttaa cttctcttga ataccggctg
     6841 cttctgattt ttgcaaataa tgagggacaa gttctaagcc gtaatcagct tttagaaggt
     6901 ctgtgggatt ttgctggaga ttttgtaaat gataacacct taacagtata tattaaacgt
     6961 ttacgcgaaa agattgagga tgacagccaa gaaccgaaaa tcataaaaac agttcgtgga
     7021 gttgggtata tggggcatgg gaataaggag tgcgggcata aagggcgcta gtataaagga
     7081 tgcgagtata aggaatgaaa atataaggga ctaactataa tggatgatgg tatatggggt
     7141 gcgagtaata tgctgagaaa taaagaaata agaatatata tagtggcttc ggtgttagtt
     7201 gcagcaacag gaactgctgc ctgctttatt attgatatca ttgcgggaat tatagtattt
     7261 tcagctctcg ttctaatgtt tgctgctttt ttgctgttta ctatttggag atacaagcag
     7321 atagaagaac tgtcccaata ccttaaacgg attgcaaacg gcgaatactc tcttgatgtt
     7381 agagataata atgagggcga gcttagcata ttaaaaagtg aaatatataa agtgactgta
     7441 actttacgtg aacaagctga gctattgaaa aaagacaagc ttttccttgc agattcaatt
     7501 tcggatattt cgcaccagct taaaactcca ataacatcaa tgtttgttat gacagaactg
     7561 atatgtgatg aaaatctgcc acaggataag cgtgtggagt tcacacaaaa tatcaggtca
     7621 caacttgaaa ggctgcaatg gcttgttgca tcactgctca aattatccaa gattgacgcg
     7681 ggtacagttg agtttagaaa ggaaaatgtt aatgtaaaag aacttatcaa aaaagctgtg
     7741 gaacacctgt taatccctat ggaaataaag gaacaaactc ttgaaataag cggtgatgag
     7801 cgtgtccatt ttattgggga tttaaactgg tcagcagagg ctgtcacaaa tattgtaaaa
     7861 aactgtattg aacatacaca ttgcggtgga ataataggta tcaattatga ccagacccca
     7921 atatatacaa taataaagat atctgataac ggtgtgggta tacataagga tgatttgccg
     7981 aatatattta agcggttttt caaaggcaaa aatgcgcata gcgacagtat aggcattggc
     8041 cttgccatgt caaagagcat cctgcaaaaa caaggaggta ttattgaggt tgccagtgaa
     8101 aaagacaaag gcacacaatt tgtaataaaa atttataaaa acattgtata gaaaaatgta
     8161 taaaaacaat atcgggggac agtcctgctt atatatatat catcggggac agtcctgttt
     8221 atataagaaa cagtaatcga atgtgtacaa gcaatttgtg acaaaattgt caccaaaata
     8281 gtcaccgtaa agtcatatta ggaagataca ctatagatac actgcatgca cattgcaaat
     8341 gcactgcagg cacactgcat gtacactata gatgcactat tagtaatacc taaattaata
     8401 ctacactact atgccaatta ggaaaacgat gccttctgag ataatttttg atagcatctg
     8461 gaagatgttg cacgggaaaa tgtctcaata gaaggccttg tgccctttcc ttaagatgta
     8521 tttcttatgg gtcagcaaaa attaccctcg agaaatgagc tttccattgg taacataaca
     8581 aagtagtact aaagagaagt taaactgaag aggagtgagt tgaaatggaa attttaagag
     8641 tggaaaatct gtctaagata tatggtaaag gagaaacagc cgtcaaggcg cttaacaacg
     8701 tttcattttc tgtaaataaa ggtgaatttg ttgcgattat cggaccgtca ggttctggga
     8761 aatcaacact tttacatatt ttaggtggtg ttgatacgcc cacaagcggc agagtttttg
     8821 ttgagaacac tgatgtttat gctcttgacg aaacaaagct tgccatattt agacgcagac
     8881 aaataggttt gatataccaa ttttataatt taataccagt actgaatgta gaagagaata
     8941 ctacccttcc gttattactg gatggacgca aggtaaataa aaatcagttt aatgaaataa
     9001 tcagcacgct tggtttgaca gatagattaa actatttacc taatcagctt tcaggagggc
     9061 agcagcaacg cgtatccatt ggcagggcat taatcaataa tcctgcactt gtacttgccg
     9121 acgagcctac gggaaatctt gacagtaaaa atagtgcgga tataatagaa cttttaaagc
     9181 tatcgaacaa aagatataat caaacattaa taataattac ccatgatgaa cgcattgcct
     9241 tgcaggcaga tcggattatt tccattgaag atgggcaaat tgccaaggat gaggtgattc
     9301 atgcatgaat atcatgaacg cgttgacact tcgtcatttg aaactaaata aaaaacgtac
     9361 tgttgttacc attatcggtg ttatattgtc ggtttccatg ataacagcag tgcctaccat
     9421 tatgacatcc ttgctgagta tgatgcagag aagtgttatt gcggatacag ggaattggca
     9481 tgtgctctat gcggatatat tgggacaaaa tgttaatata gttatgaatg ataaaaacac
     9541 tgatacagca gcacttagca aggatgtagg atatgctgta ttgagaggat ctaaaaacaa
     9601 gaacaaaccc tatcttttta taaaatccta tgatgagcag ggattcataa attttggttt
     9661 aaagttaatt gaaggacgtt tcccccagag ctcaaatgaa atcgtcatat cttctcatgt
     9721 tgcagaaaat ggtggtgtgg aatataaggt gggcgacgtt ctcaaattgg atattggcca
     9781 gcgttatatg gttgaagatg aaaagatatc tacacttggg cagacttcag gttttaagga
     9841 agcaagtgat gataataagg gcgaaaagtt tgcagagcaa tttacaaaag aatatactgt
     9901 aacaggaatt ataagcaggc cgggatttga gccttattgg gcaccggggt atacggtgat
     9961 ttcctacctt gaaaaaaatg aacttacgcc aaacgacact gtcaatgtat cggtagcttg
    10021 gaaaagtatt aataaaaaag caaatgagca tgcaaataag ctttttgaaa gcataaatac
    10081 ttctggtagc agcagtataa cctataatcg gcaacttttg cggtactacg gaattattgg
    10141 tgaagattta ttaacaatgc tatataccct tgcagctata gttacactcc tcatcattac
    10201 aggttctgtg gcgcttatat ataattcatt tgcaatttca atctcggaga ggagccgcca
    10261 tcttggtatg ctggcaagtg tgggggcaac aaaaaagcaa aaaagccgtt ctgtattttt
    10321 tgaaggcttt gttgttggaa taataggtat accattaggt gtatttttcg gtacattagg
    10381 aataggtgtt acttttgttc ttgtacagcc tttgatagaa ggcttaaaca acacaggggt
    10441 taaattaaca ctggtaacct cccccggcac aattgtagtt gctgtgctgc tttcattgct
    10501 tactattttt gtatcagcct atgttccggc aagacgtgca tctaaggtat cgcctgttga
    10561 tgccttgcgt caatcacaag acatcaagct tacaagtcga gctgtaaaaa cctcgagatt
    10621 aactcgtaaa atatttggct ttgaagcaga actgggactt aagaatttaa aacgaaacag
    10681 ccgcagatat aaagctaccg ttttttcact tgttattagc atagtgctgt ttttatctgt
    10741 ctcatctctg tcattgctta ctcgaaaatc agtagatatt gtatccggcg gttaccctta
    10801 tgatgtaaga gtgtttgtgg tatcatctgc tacagcacag gagaaaaagg acttttatac
    10861 tgcaataaca aagatggcgt atgcggataa aagtgtaatt gagcaaacta tgaatgctgt
    10921 attcactgca gatggcaaat taataccaga tcaaattaaa gaaattattg agccaaatta
    10981 taaagatata gagccaaatt ataaagatat agagccaaat tataaagaaa ataaatctga
    11041 gacaaatact aatacctttg atatggaagc cgaaataaag tctattgatg aagcagctct
    11101 tgaaaggtat gcgaaagaaa taggagttga tcctgcccgt ttaaaagata cggaaaaccc
    11161 ctgtggtgta ttggttaaca ctgtgactat taaagttggt aataaatatt cgagaatgaa
    11221 acactttaat attgagcagg gtcaaaaatt gaaattcatt caccatatat atgacggtaa
    11281 gagttccgga gaacctcaaa aaccggggga acttggagga cctggagaac ctgaagaata
    11341 tggtaccata ttagaaatag cagcccttgc cgatagtaca ccgattggag aaacaacatt
    11401 tactgatcct ttacgggcag tgtttattgt ttcagaagag gtatacgctc atatgcaggc
    11461 gaagctgccg gaagagtctc ataatacaag agttcttctg ctcattaaga gttctaatcc
    11521 cagcaagctg gttaaaggta tagaagaata tcaaaaacaa acatcaattg caagtgtaga
    11581 tatttttgat gttgcagcac ttgataaagc caacagacag tttgagactt ttatatttgt
    11641 attcttttac ggctttgtag cactcattac tgctatttgt gcagcaaata tttttaacac
    11701 aatatcaacc agcatatccc ttcgtaagcg tgaatttgca atgttaaaat cagttggcat
    11761 gacacctgag ggatttaata aaatgataaa ttatgaaagc ttgttttatg gtattaaggc
    11821 tttgctctat ggtttgccca ttagctttgg gattatgtat ttaatttaca gagttcttag
    11881 caattcattc gggtttgcat ttgctattcc ctggagcagt gttttagcag cggtgattgt
    11941 ggtgtttgca cttgttggat caacaatgct atatgcaagt tcaaagatta agaaagagaa
    12001 tattattgat gcattgaaaa cagaaaatat ataaatgtga attcagagga caagtagggg
    12061 gatagtactc caggacatat aagggacagt tctgcataat gtatagaatg taatgtatag
    12121 aatgtaatgt atagaatgta gaactagaat gtagaactgt ccctctactt cactacttca
    12181 gtgctgcccc catacttcac tttatgcagg actgtcccca tgcttcattc cggtatatat
    12241 aagaatagcg tctcttaaaa attttgcagc accgggctgg tttttgtcat agaatgctgt
    12301 aaaccgatca tcatctacat acatttgagc aatgcctgca tgtgcttctt tagtatagct
    12361 gtcccagaaa aagcttaacc actggcgatg caagtctgct gctttttggg ctaaatctcc
    12421 tgctgggttg ccggttttaa aagctgtatg cagtgtttca ataacctcat ttccaagcct
    12481 gacagactct tcatactgtt cctctgacat acctttgatt ttttgaattg atttgttaaa
    12541 agcttggtca ccatattttt ctcgagcttc aatgccatac tttctctcgt tatcatcaat
    12601 cattttttgc ttaaaacctt caaatttctc tgtatcagtc atttttactc tcccttctgc
    12661 caatgctata gttttgtcca cgttggcaat cagcaaatcc aattgttttc ttttttcaag
    12721 aagcttctcc cgatgctcct gcagtgcttt atgcttgtca aaagagggca ctgttacgat
    12781 atctttaatt tcgttcaagc ttacaccaag ttccctgtag aaaagtatct gctgtaatct
    12841 gtcaacctct gattgcccat aaatacgata tcctgaagaa ttgattcttg ccggcttaag
    12901 aatcccgatt tcatcgtaat accgtaatgt tcttgtgctg ataccagcaa ttttcccaag
    12961 cttttgcacc gtatattcca tatgccaacc tcctgacaat attagtataa acccttacgt
    13021 aacgttaatg tcaataagtt ttaaataaaa agttcagaat taatttagca ttagttatag
    13081 cagaatataa ccgggccgcc ccatgcttca ttttatacag gactgtcccc gtacttcatt
    13141 ttatgcagga ctgtcccctt tctttcagga actttttcat tcataaagga ataatctagt
    13201 atagatattc tttaaataaa tgtggtgata tatatgtatc ctaatatata tcataataat
    13261 agagttggaa taattccata tgtacttcag cctaatgata acttatggct gttagcccaa
    13321 agatataata ctaccgtata tgatattgct gctgcaaacc ccggcataga ttttaacttc
    13381 ctgcaggctg ggcagcttat atacattcgg cctggattcg gacagtattc cacagtttca
    13441 aatattggta ctccagagac tccggtttgt gggattaata aaaaagagtt aaatttaatc
    13501 aattatttgc gtatgctttg ggaagagcat gttacctaga cgagattaac tattgtaagt
    13561 atagtttttg gtttgccgga tgtggacctt gttaccagca ggcttctcca gaatccaaaa
    13621 gattttgaag aagctttaaa gcctttctac ggaaacaaaa ttgcctccag ctttgccgat
    13681 ttatttacaa atcatcttgt cattgctgca cagcttgtta aggcagccaa agctggtgat
    13741 agcaatgctg caaaagatgc cgagaaaagg tggtattcta atgcagataa aatagctgct
    13801 tttctagcag gcattaatcc ttattggtct caggataaat ggaaaagaat gctgtatgaa
    13861 cacttggcaa tgaccaaatc tgaagcagtt tatatgctaa ctaaaaacta tggggctggc
    13921 attaccgaat atgataaaat tgaaaagcaa gctctgaaaa tggctgatgt aatggcttat
    13981 ggtattataa agcaattccc ggatatattt gcatattaaa taaagtttat tttatagtat
    14041 gaagttccaa atacaggtgc atccaatgat atagaacaag caacaaggaa agcaatgtcc
    14101 gtataaatgt tttttacaat agtatggaac aggaaaaaca tttccgtgac aattaattac
    14161 atagtataca caaaatagtt gaaatggaac aataagccgt atataatatt gttgataagt
    14221 gtaataaaat attataaaat aggagtgtag atatggatta tttgtgggtg ctagagccgg
    14281 aggcggaata tggtactata ggggacaaac ttgatactgc agggaacgag catgatacag
    14341 tatggaacaa acatgataag ggatataaat atcttctgtc agtaaagagg gtatttatgc
    14401 aattgctaag aagttttgta gacgagagtt gggtaggtag tatagatgag gatagcatag
    14461 agtatataaa taaatctttt atactacaag attttaaggg aaaagaagct gatatagtat
    14521 ataagcttaa gattgacgga agagaagtat ttttctatat tttacttgag ttacaaagta
    14581 ctgtcgactt tcaaatgcca tatagattat tacagtacat gttggaaata tggcgtacta
    14641 ttttgaaaga tacaaagaaa gcaatagaaa aacagaaaga agcatctaag gataatacag
    14701 aaaatagatc agaaggtaaa ataggagatg aagttgagag aaaaatagaa gttgaaacaa
    14761 agaatgaagt agagaatgac ataaatgata tagaaaaaaa taaggaaatg gaaataggga
    14821 aagatggaaa agatttcaga ctacctgtaa taattccttg catattatat aatggtgagt
    14881 acaactggac agcacccaga agttccaacg atatactaat agcaaatggg ttatttgatg
    14941 attatataat agactttaat tatatacttt ttgatgtagt aagatatgat gaagaagaat
    15001 tactaaggct tggaaattta gtaggtgcag tattttacat agaccaaaag caacaatatg
    15061 atgagattat tgaaaggcta aaaaaccttg tagatcagtt aaaagactta agagaagagg
    15121 actttgaact atttaaaata tggcttaaaa atgtagtaac acgtggtatg ccaaaagaag
    15181 aagccaagga aatagaaaaa ataatcgaaa gtaataagga ggctgatagt atggtttata
    15241 atatggagat agcaatacgt aacaaaatga aagaagtgga acttaaagga aaacaagaag
    15301 ggaaattaga tgtcgcaaaa aacttatttg atgcgggaat ggatatagag caaataggca
    15361 tagttaccaa tattcctgta gatgaattac aaaaacttat tgtaaaataa gagagcagtt
    15421 cctttatcct gttcccaata aatagaaaaa gtaggtataa tgcccgattt tagggggcgc
    15481 atacctgttt ttttttacct atattttgct tcgcgtttat ttcatgtata ttaactgctt
    15541 tctgctgtta aaaaagcccc tatatgaaag gtaacaggca ataaaagaag atatagaggt
    15601 agtagagagc agcataaacg taaccattat ttgatatttt attgcttcca gcggcggtgt
    15661 acctgcaagg atcagccctg tcatcatacc tggaagagat acgatgccta atgtttttgc
    15721 agagtcaatc gttggcagca tacctgtttt tatagagttc cgaattattt cgatagagga
    15781 aggcaatata tctgctccta atgaaagctt tgtttcaact tcttcacgtt tgtctttaaa
    15841 atctgaagtc atctgtttat agcacaatcc caatgcaacc attgaattac tgataatcat
    15901 accactgaca ggaataattt gataaggctc atattgtact gcttttgaca aaataagaat
    15961 cgttaaagta actaaagttc ctgttaaaat ggacaaaaag gatattaaaa gtccattttt
    16021 gatagccttt cctctttttg cagcgttata tgcagcgttg aaggtcataa aaagcagcaa
    16081 tagagttgta aaaacaggat ttttataacc aaaaacatat tcaagaatat atcccacagc
    16141 aataagctga attacagctc tgacaacgct aataatagtt tccttctcaa gtttaagttt
    16201 ttgccagtag gagaagaaaa gtgacaccat tacaagagta gaaacaataa gcaatgatgt
    16261 gatatttatt gtatttgaac cattcatttt agctccattc cttccttttc tgctaatacc
    16321 tgcttcaaac taatacatcc aatgatttaa tttcgccagc ctcaatagtt aataacttgt
    16381 tggcatgttt tctgctttgt tcagggttat gtgttatcca aagcactgtc atgccttccc
    16441 tgtttaatga aacaattaca ttttcaacaa tttcagtatt gttcacatca agtgcagagg
    16501 tgatttcatc caaaagcagt atttcaggtt tgaaaagcaa acttctaatc agtgccagcc
    16561 tttgcttttc tccccctgaa agacttttta catcttcatt tagaaaatcc gctgacatat
    16621 gaaatattga aaatagttcg tttatttttt taatgtcagg tttcatattc cttatagaaa
    16681 aagggaaatt tatattatcc aatactgtat tgccaaaaag ataaggcgtt tggaagcaat
    16741 acactatact tttcctcaat tcagtaggac tgtattctgt aatatttttg tccttaaagg
    16801 ttatattacc tctggtagga cttatcaaat ggctgcaaag cttaaaaaat gtacttttac
    16861 cactgcccga tggaccaact actgaaataa aatcccctga ctcaatgtct acacatatat
    16921 ttttaagaat ggttttacca tcattatcaa agtacacatt ttcaaattgt agtaatgaca
    16981 aaccaacacc tccggaagta ttgataaagc tatttgaaaa caatctgtct tcttctgtat
    17041 gcttttctac agctatttca tattttcaag ttatctgaat atcataatat cataagatgt
    17101 gagctatgag ctgtgacatc caaattatat taataataat acttttagtt attaacatca
    17161 atatgctatt tctctcaaaa gttaataaaa tgagaaactt atctggactt tttgcagcaa
    17221 tttccatttt gggtgcataa ttggccttaa atcaatgcaa ctcaatatca tgttgacggt
    17281 gaatgagttc ttagactaaa attatcttat aaatggtata ataatagata ttaataattt
    17341 attataaaac aaagaaatag tggaatggtg ggatggtggt taattatgat tacaactaat
    17401 aatgattggg acgaattgct taaggatgaa tttcaaaagg aatatttcat agaattaaag
    17461 cagtttctta taaaagaata tagtacatag atcatatacc c
//
LOCUS       DAOSDD010000079        17481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1321_length_17481_cov_4.532021, whole genome shotgun sequence.
ACCESSION   DAOSDD010000079 DAOSDD010000000
VERSION     DAOSDD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17481
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1321_length_17481_cov_4.532021"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..416)
                     /locus_tag="PK733_08740"
     CDS             complement(276..416)
                     /locus_tag="PK733_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10664.1"
                     /translation="MVYRLFGNTTHDQAVVGVDDEDKNADDIHILPGFTEEEVASAHA
                     VV"
     gene            complement(524..2713)
                     /locus_tag="PK733_08745"
     CDS             complement(524..2713)
                     /locus_tag="PK733_08745"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOJ10665.1"
                     /translation="MLSIMVYLVGLGSIFSLIFAYYNFRKGTKMAQGTPIMQDIAKSI
                     REGADAFLKREFSISIPIIFGIAVLFGVFFTHWAGLAFLVGAAMSSFAGLVGMKAAVI
                     YNERVANEARLGVERKDPSALGKALNVAFRGGSVMGLSVGGFAMFGLLMVYLIVGMLF
                     QYANPENLVETTSWVGLKFVLFPQVLACYSLGCSSIAVFNRLGGGIYTKGADMGADLV
                     GKTELGLPEDDPRNPAVIADCVGDNVGDVNGNGSDLLESTIGAVVSSSVLPTAIHTAA
                     INSSKPISTELIVRMMQYPLAFMGIGLLACVLGIAYVLIRKPSNKLSRELNISLWSSA
                     AIIAAATFVLSYLFFSGQDHTGVDFRFGWISPWLSAITGIIAGIALGMLAERYTSEQY
                     KETKNVAHLAKEGPALVIIGGTSTGWRSCLPACIVLGCAIIISSLFSGLYGSAIAAVG
                     MLSFVVATVTVDTYGPISDNAGGIAEMSQLDEDVRNITDSLDSQGNTTAAIGKGFAIG
                     SGVLAALAMMMSFANSGASGGELNILNYLALGGAFMGVGVMHWLSGVMLNAVCHNAEQ
                     MVDEVRRQMRENPLILAGEVKPDSNRCIDICTKSAIAEMKKPVLVSLLSPIIGGFIFG
                     PLFVGGFLLGTILDAVVMATSTANSGGTWDNAKKYIKSLKADLIKEYGEKRYNEIHNA
                     GIIGDTVGDGYKDVVGPNQDIMIKMMATVSVIMLPVMNTFNLISLLK"
     gene            3117..4160
                     /locus_tag="PK733_08750"
     CDS             3117..4160
                     /locus_tag="PK733_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10666.1"
                     /translation="MAVQTVLGAIESSELGIISPHEHVFIDLRGFFTEGYIKGCVATS
                     TDSVTIEKLGILNRDPYAIKDNLIIDDMEIQIKELLRFKQAGGSTVVDATTLGIGRDP
                     IALKKIAIATGLNIVIGTGFYVGTTHSKELLQMEINEIANIMQSEIEIGIDGTDIRAG
                     VIGEIGISEVFSDSEIRVLKASAIAQKTSGLGILVHINPWTTNGIKASKILLDDGIKP
                     EKIAICHVDVENNKEYIMELLRAGIFIEFDNFGKEYYVQRQVRRSGYGLFAKDTERVT
                     LLKQLIDKGYINQILLSCDVCLKTLLVRYGGWGYAHILENIVEMMEDEGISHAMLDIM
                     LRENPARFLDSQT"
     gene            4208..5383
                     /locus_tag="PK733_08755"
     CDS             4208..5383
                     /locus_tag="PK733_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOJ10667.1"
                     /translation="MPHEIPVSEFRQRIINLQTNMQSEGYDLLLAYGNEAEPQFVRYL
                     CDYWPSFETAGVLIPAQGDPLLLIGPESLTYASDRSKVEKIRLLKAFRESSEPEYPGK
                     KLDTFSTVINECIKGGELKKFGVAGLSLMTVGTYFDLAETLKDYGNLEITKADSLITN
                     LRMIKSENELACMREAANITRKTFDYVLNNIKPGMTENQVIGLALGKMHQLGADRESY
                     PMWALTGKGSNQAISRARHSVIQSGDMMQIQVGARVGGYASSIGRPVVFGKATPEQHE
                     LIMAGYTAYKAVIDKLKANIPAYEVALHHEKTVKDIGYGDWLLYGPCHGNGLMEGEPP
                     WIETNAKWLLQENMTFCVDIFLGSNITQTGLRIEDVVRITSSGVEQLTNYPCELFEL"
     gene            5440..6231
                     /locus_tag="PK733_08760"
     CDS             5440..6231
                     /locus_tag="PK733_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10668.1"
                     /translation="MSTIKDKLWIWGQTPGTHHGETNPYKLPGQNRMTSLEGAYYLGV
                     PNCCRVVSLGLPNPPFDQEAMVLDTLDRVIWSIIGCNASKRSNDMEEVIKIAKKHPNV
                     IGGIVDDFMRKSRLEEYPPHAIRGLRERLTEEVGRKMELWTVIYTHEFDLSIKEHLAE
                     FDVATMWTWWAKNIVDLDKNLVKLRSLFGAEKPILAGCYMWDYGGQNPMPIDLMKYQL
                     DKYYAWLKSKEIDGIIFCSNCIADLGLEAVDITREWIEKVGGELL"
     gene            complement(6411..7361)
                     /locus_tag="PK733_08765"
     CDS             complement(6411..7361)
                     /locus_tag="PK733_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10669.1"
                     /translation="MYSAQINTNSAIPIFAGKDFFKRDRNIHIHISTEFPAYIGIMHK
                     HKFIEIVYIISGRGTHIVGNDLYTVNKGDLFVINYDTPHAFCADSNTEENFLAYDLMF
                     TPDFLDPSMVGCQGFESLQSSFLFHDFCVNEQKPVYHLQFSANNYDLLGILFNKIYLE
                     YNSKQKGYVDLARAYVIELILKITRTLGNVSESNISYQKVHIVNTVLKYLQMYYQKNI
                     TLENLSNEMYLHKDYISKTCKNVTGLPVSILLQKMRIEQACLLLRTTDKKVVEIAKCC
                     GFNDIKFFYKIFKKMMGVTPGDYRNKLTSINCQNLHIQDK"
     gene            complement(7394..9571)
                     /locus_tag="PK733_08770"
     CDS             complement(7394..9571)
                     /locus_tag="PK733_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10670.1"
                     /translation="MKLIGTKSQYIMSYASIALVSCILIGLVFFRISVNELNEAATRE
                     QINKLELATEYLENQQKLMMEIAYKIQNTIYYKPSQLEVNKYKDIILLKDFVLYKNYI
                     PVSQEYFLLYHDSNSVYGPSTTNTFQHYMQHIVNITDYQGIYDKLNSVSEFTVICPVE
                     NENVFLLAFPIYLTEKMNLVGDATLCFVVQKANVLKRIKETIGTFNGDIHIYYDNLLI
                     ISSNTLELLKPSNVSQTSSKSNETKYIKLNSYSSNFTLALKVPEFGIYDRLGKYVTFS
                     KALIIAITAIMIIFAALVAYYSYRPIKRLVNQYEPFFNMELKNKNEITQISLMLNSAF
                     EKSMLTSNKLAEEMTRLENQRKIILRQFIQLLLSGEIDENISESMRYLDIPFACPYYN
                     TFVIQVDPEKAYKMDHLMTTIENLSDDTMILYAVKFKHLYSFSVLTIISEKTQTKKIK
                     GFIEAIGEVEKINLKISVGLICDDIKRIQESIMDAIFKQRCDDFPQQFGQRDIANSFL
                     YNDSYLHQMTVGLENGDSIETINIFEQFIRDVKDSPLSPIIQHCIFSDVVSMLIRVSS
                     NYRISIPQEIIGTLMFLKDADSFLAETTKLIIDICTRIRFNIEEKEKSARLEIVEYIN
                     NNCGDYNMDLLFLSDKFNLSGKQISRIINEITGMPYKEYLTYVRINLARKMLVEHDLS
                     VAEISRHVGFMNTSYFIGVFRKTTGLTPAIYKKRYSIVTASSN"
     gene            complement(9988..11049)
                     /locus_tag="PK733_08775"
     CDS             complement(9988..11049)
                     /locus_tag="PK733_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ10671.1"
                     /translation="MFKYNCKKFVILVLTVYCIVSLLVSCTNSSEGLQSIQKKTQDKS
                     PDTPETLENNGTLPTSRLSEVLDPNKYAEQFTIRAFNVKHEEKDGNIITGDCSLLIAP
                     DGTTMLIDAGYKSDFPSIMEDIKKIGVTKLDYIVATHMHSDHVGGIPYALETMPVGKV
                     MTTKYTDPRYGSSSSLLEALQKRNMQLHVLKEEDSFFLGEGVKVEVLNPENRDDVEFS
                     DNDYLIQHLNNELSLVLKITYGDHIFLFTGDIQTMTEFRLAEKYGEQFEVDLLQVAHH
                     GYHSSSSSVFLKAFSPKMAWIPQCSTPPFSVYSRIKKVCSNIYITGLDGILLFASNGK
                     EITVITEKDRESEDLKLYN"
     gene            complement(11070..11966)
                     /locus_tag="PK733_08780"
     CDS             complement(11070..11966)
                     /locus_tag="PK733_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ10672.1"
                     /translation="MIMKLSTSTNLFSSVMKNGELLSILDRITRCYKAGFDVIDINFC
                     PYKGDFGFLRNNNWEETVNKMNEIKEELSLEYTQSHIPFYGALEPFCFENPNGEWMEE
                     MVRRSIMASGMLGVKWAVMHPGTAVDSNCSQSISKQRNIEYFAPWIEFAEKQNVGIAI
                     ENMADLPNHKIVRRYGSDPEELCDLVDYFNSENVGVCWDTGHANLMYYEQTISLKVIG
                     SRLKALHINDNHNKDDEHLIPFYGNIKWDSIMKTLIEIKYDGNFTYEIQGHVQRLPDE
                     LVDEIAIHAINIGNYLLNSTNS"
     gene            complement(12037..12948)
                     /locus_tag="PK733_08785"
     CDS             complement(12037..12948)
                     /locus_tag="PK733_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10673.1"
                     /translation="MKKTSSDIIFDSINITFVALIIIVMVYPLYFVIIASLSDPYAVA
                     NGKVTFWIKGLSFDAYKYTFKHKEIWLGYMNTIFYTLVGTGLNLLLTIPTAYSLSKKK
                     LPGRSFISWYFLFTMYFGGGLIPTYLLVRDLNLLNKPYTIPLLGGISIYNTIVTRIFY
                     QTTIPDELYEAAYIDGASDFRQFFQIALPLSAPIIAVITLFYGVGRWNDYYTALIYVS
                     KSDYYPLQMVLRSILIQNKSTLDAIDTVSTQFVDDEYVAKVARQAYMAEAMKYSLIII
                     ASAPLLVAYPFVQKHFVKGVMIGSLKG"
     gene            complement(13018..13941)
                     /locus_tag="PK733_08790"
     CDS             complement(13018..13941)
                     /locus_tag="PK733_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10674.1"
                     /translation="MRNIQGKFSNNLKVILSYWQLYALLLPAIIYVFIFSYIPMYGVQ
                     IAFKDYRTSIGIWGSKWVGFKNFKRFVTFPNFWLYIKNTMSISIYSISTFPCSIILAL
                     SINELQKAKFKRFVQMITYAPHFISTVVLCGMIHIFLDRNSGIVNTLIKALGGTAIPF
                     MSKPQCFRSIYVWSGVWQGIGWGTIIYIATLAGIQQELVEAARIDGANRFHIIRHINI
                     PHIMPTVMILLIMNFGSILSVGFHKIYLLQNPLNLSVSQVISTYVYQVGLISGQFSYS
                     AAIGLFNTAINLSLLIIVNLIVKKASSISLW"
     gene            complement(14348..16099)
                     /locus_tag="PK733_08795"
     CDS             complement(14348..16099)
                     /locus_tag="PK733_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10675.1"
                     /translation="MKRTLIALTLVIIMLTSIISGCTVNKEIKSDTKTASNGNDSTTT
                     PDALDGKLEHINYASEIPIVKEGHKVSLRFCLLTNPALYASVENCWFFKFIEKFLNID
                     PKIEAVPVEAHSERLNLMFASKDVPDIVLGFGINTREIVKYGVNEGQLLSLNDYINPT
                     YMPNVYKLSKEDPGLMASATAPDGNVYKIPRILDDEGTVEMVNIRQSWLDKLNLESPS
                     TLDEFIETMRIFKKEDVDGHGSENVYPLGGSLNYLNPLATIINAMGYNFPYSWASAAK
                     GNGLTIAIRDGKATFPCNDDIFYDFLSIAKTLYDEELISPDFFTMDQTKVNAMAVEGV
                     YGYMCQPMHVLLTEPKKYQEWFYIKPLTSKWNDTPQVPQTSKYDMQGIAISANTKYPE
                     VCARFIDLYYGDNCFFMWNGAMDGTEETLGFKGWIWDESANSEYYPDEPSAYNYISNI
                     LMPYSLQFGLHSWRGEKMAKYAGKEYKKGLDISTLDLNQGGNWNTYGRVKMFYPYAKP
                     AYPPIVYRSEEDLEMLAEYSAVIGPYVTEQVALFITGTRSLNEFEQYLKELKSLGMDE
                     YEQYYIDEYQSYLEKQK"
     gene            16577..16792
                     /locus_tag="PK733_08800"
     CDS             16577..16792
                     /locus_tag="PK733_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10676.1"
                     /translation="MKNILMSAKDYHEAYPNGHVQDNAPDDTALHADKRHAGAFSRPA
                     AGSAGIRAGIDLFADFYAAVRMIDGIV"
BASE COUNT         5650 a         3287 c         2963 g         5581 t
ORIGIN      
        1 ctaaaaacac aacacaaact gcacattaag ggatataaca acccctcctg aatatgtgaa
       61 tgactttatc taaagttttc gtatgcttat gctgatttgg tgaaatcttt gcattgttaa
      121 taatatctta tactcattac caactttagt cattttacaa aaaagaaaaa accgtcaatc
      181 aaggaattta tatctcgatt gacggtcaac taccaatact atgtttatat atatgtttca
      241 acatccgatt ttaatataat tgaacgataa tgttcttaaa ctacagcatg ggctgaggct
      301 acttcctctt cagtaaaccc tggtaatata tgaatgtcat cagcgttctt atcttcatca
      361 tctacaccaa caactgcttg atcatgtgtt gtattaccaa acaacctgta aaccatatcg
      421 cttcgtacat aggtaagaga accaactatc cattaagagg ccttcagata gccgaactca
      481 ccattgtcga atttgacagc gataaagctg caatttccga ttattacttc aataaggaga
      541 taaggttgaa tgtgttcatg accggcagca tgattacaga aaccgtggcc atcatcttga
      601 tcatgatatc ctgattgggg ccaacaacat ccttatatcc atctccaaca gtatcaccga
      661 taatgccggc attatgaatc tcattatagc gtttttcacc atattcctta attaaatcag
      721 cctttagaga cttaatatat tttttcgcat tatcccatgt accgccacta tttgctgttg
      781 aagttgccat tacaactgca tctaagattg ttccaagcaa aaatccgcct acaaacaaag
      841 gtccgaatat aaagccgcca atgattggag atagaagaga aacaagcaca ggctttttca
      901 tctcggcaat agcacttttc gtacagatat caatgcaacg attggagtcc ggcttcacct
      961 ctccagccaa aatgagtgga ttctcacgca tttgtctgcg tacctcatct accatctgct
     1021 cggcattgtg gcatactgcg ttaagcatta cccctgaaag ccagtgcata acaccaacac
     1081 ccataaaggc accacccaag gcaaggtagt taagtatatt gagttcaccg ccgctagctc
     1141 cactatttgc aaaggacatc atcatagcca gagctgcaag cacaccggaa ccgattgcaa
     1201 agcccttgcc tatagctgcc gtagtgttac cctgagagtc gagactatcg gtgatgttgc
     1261 gtacgtcttc atccaattga gacatttctg caataccacc ggcattgtca gaaatcggac
     1321 cgtaagtatc cactgtaacc gttgctacaa caaaggaaag catgcctact gccgcaatgg
     1381 cagaaccata tagtcctgaa aagagacttg aaataatgat agcacagccc aggacgatgc
     1441 aggcaggcaa acaactacgc caaccggtac tcgtaccgcc gatgatgact aacgccggac
     1501 cctctttggc cagatgggct acattctttg tttctttata ttgttcgctt gtgtagcgct
     1561 cagccaacat acctagtgcg atacctgcga taattccggt tatagcactt agccagggag
     1621 aaatccatcc gaaccggaag tcaacgcctg tatgatcctg accgctaaag aaaagatagc
     1681 ttagtacaaa ggtggcggca gcgattattg ctgcagaact ccacaaagag atgttcaatt
     1741 ctctgctaag cttattgctt ggttttctaa tcaatacata ggcaatgcct aaaacgcagg
     1801 caagaagacc aatacccatg aaagcaagcg gatattgcat catgcggacg ataagttctg
     1861 tactgatagg tttagaggaa ttaatggctg cggtatgaat tgcagtcggc agaacagatg
     1921 acgacactac cgcaccaatg gtgctttcca gaagatccga accattacca ttcacatcgc
     1981 cgacgttatc tccaacacag tctgcgatga ctgccgggtt tcgcggatcg tcttccggaa
     2041 gccccaactc agtcttgccc acaagatcag cacccatgtc tgcaccctta gtatagattc
     2101 cgccgccaag acgattaaac actgcgattg aagaacaacc cagggaatag catgctagca
     2161 cctgagggaa aagcacaaat ttcagaccaa cccaactggt cgtttcaaca aggttttccg
     2221 gattggcata ctgaaacagc atacccacaa ttaagtacac catcaacagc ccaaacatgg
     2281 caaacccgcc aactgacaaa cccattacag atccgccgcg aaatgcaacg tttagtgcct
     2341 ttcccaaggc tgatgggtct tttctttcta caccaagacg ggcttcgttg gcaacacgct
     2401 cgttatatat aactgcagct ttcatgccaa ccaaaccggc aaaagagctc atggctgcgc
     2461 caaccaagaa cgccaaccct gcccaatgcg taaagaacac accaaataat actgcaatac
     2521 cgaatattat tggaatacta atagaaaact ctctcttcaa aaatgcatct gcgccttctc
     2581 tgatcgattt cgcgatatcc tgcattattg gagtaccttg tgccatcttt gttcctttac
     2641 ggaaattata atatgcaaag ataagagaga aaatagaacc taaccctaca agatagacca
     2701 tgattgataa cattaactga atacccccta aatatcgaca atattttttc ctttaaaaat
     2761 cattatacca gcagcttaaa cggaaagcaa tatatatcct ttttaagttt tctgagtttt
     2821 ctgccatccc cgttgcattt tgtgccgggc acaaaacaca acacctgcat atctactaac
     2881 tttaatacaa tgaggtacta tttcccaaca ttgaacctgg acacttttca gatgacaggg
     2941 ttgctgatac ctttaaacag ataatatcat tatgatatat cttaataccc taataagata
     3001 tcctaatgca ccattgtcta gaactacaga aacaattaaa atataaaata gtaagggtaa
     3061 aaagatattt tttgcatatc tctgaaaata gtagataaaa agcgggagtg agcataatgg
     3121 cggttcaaac agtattagga gcaatagaga gtagtgaact gggtatcatt tctccacatg
     3181 aacacgtatt cattgatctc aggggttttt ttacagaggg ttatataaaa gggtgtgtag
     3241 caacatcaac ggactcggta actatcgaaa aactaggaat tcttaataga gatccgtatg
     3301 ctatcaagga caatttaatt atagatgata tggaaataca aataaaggaa ctccttcgtt
     3361 ttaagcaggc aggaggatcc actgttgtag atgctactac tttgggaata ggacgtgatc
     3421 caatagcatt aaaaaaaata gctattgcaa ctggacttaa tattgtaata gggacagggt
     3481 tttatgttgg gacaacgcat agtaaagaac ttcttcagat ggagattaat gaaattgcta
     3541 atattatgca aagtgaaatt gaaataggta ttgatggaac cgatattagg gcaggtgtta
     3601 tcggtgaaat aggtataagt gaagtattta gcgattcaga gattagagtt ttaaaagctt
     3661 ctgcaatcgc acagaaaaca agcggtttgg gcattcttgt tcatatcaat ccgtggacta
     3721 caaatggaat aaaggcttca aaaatccttt tggatgatgg tattaaacct gaaaaaattg
     3781 ctatttgcca tgtagatgta gaaaataata aagagtatat catggaatta ctgagggcgg
     3841 ggatatttat tgaattcgat aacttcggta aggaatatta tgtccaacga caagtacggc
     3901 gatctggata tggactattt gcaaaggata cagaacgtgt aaccctattg aagcagttaa
     3961 ttgacaaggg atatattaat cagatccttc ttagctgtga tgtttgctta aaaactttac
     4021 ttgttagata tggtggttgg gggtatgctc atatccttga aaatatagta gaaatgatgg
     4081 aagacgaagg aataagtcat gcaatgctag atataatgtt acgagaaaac cctgctagat
     4141 ttctagattc tcagacataa aaatattgtt ttaaatatta tttaaatata ttacaggggg
     4201 tatcaaaatg ccacatgaaa taccagttag tgaatttagg caaagaatta taaaccttca
     4261 gacaaatatg caaagtgaag gatatgacct tttactggca tacgggaatg aagcagagcc
     4321 gcagtttgta agatatttat gtgattattg gccttcgttt gagacggcgg gagttttaat
     4381 acctgcgcag ggtgatccct tgctgctgat aggccctgaa agtttaacat atgcatcaga
     4441 ccgttcaaag gtagaaaaaa ttcgtttact caaagcattt agggaatcct cagagccaga
     4501 atatcccggt aaaaaactgg atacctttag cacagttata aatgagtgca taaagggtgg
     4561 tgaattaaag aaatttggtg tcgctgggct tagtctaatg acagtaggta cttactttga
     4621 ccttgctgaa acattaaaag attatggcaa tttagaaatt acaaaagcgg attcacttat
     4681 tactaatcta agaatgataa aaagtgaaaa tgaacttgca tgtatgcgtg aagcagctaa
     4741 tattacaaga aaaacttttg actatgtact aaacaatatt aaacctggaa tgactgaaaa
     4801 tcaggttata ggccttgcac ttggaaaaat gcatcaacta ggggcagata gggagtctta
     4861 cccaatgtgg gctttgaccg gtaaagggag caaccaggct ataagtcggg cacgccatag
     4921 tgttatccag tctggagata tgatgcaaat ccaggtgggt gcacgagttg gagggtatgc
     4981 ttcttccata ggccgcccag ttgtatttgg gaaagcaact ccagagcagc atgagttgat
     5041 aatggctgga tatactgcct ataaagcagt tattgataaa ttaaaagcaa atatacccgc
     5101 atatgaggta gctttgcatc atgaaaaaac agtaaaagat ataggatatg gggattggct
     5161 cctttatgga ccttgccatg gtaatggact catggaggga gaacctcctt ggatagaaac
     5221 taatgcaaaa tggttacttc aagaaaatat gacgttttgc gtagatatat tcttaggaag
     5281 caatataaca caaactggat tacggattga agacgttgtc cgtatcacat ccagtggggt
     5341 tgaacaactt acaaattatc catgcgagtt gtttgaatta taggaaaatt tattgttata
     5401 tagaaaatat aagatagcta ataaaggagt gaaataaaat tgtctacaat aaaagataaa
     5461 ctttggatat ggggtcaaac accaggaact catcatggtg aaacaaatcc gtacaaactg
     5521 ccaggtcaaa atagaatgac gtcattagaa ggtgcatatt atcttggggt acctaattgt
     5581 tgccgggtag tttcacttgg tcttcctaat ccgccatttg atcaggaagc aatggtcctc
     5641 gatacacttg atagggtcat ctggtcgata ataggctgca atgcttcaaa acggagtaat
     5701 gatatggagg aagttataaa aattgcaaaa aaacatccta atgtaatagg tggaatagtg
     5761 gatgatttta tgagaaagtc acgtctagaa gaatatccac cacatgcaat caggggttta
     5821 cgtgaacgtt tgactgagga agttggccgt aagatggagt tgtggacagt catatatact
     5881 catgaatttg acctttctat taaagaacat ctagctgaat ttgatgtagc aacaatgtgg
     5941 acatggtggg caaagaatat tgttgatctc gataaaaatt tagtaaaatt aagaagtcta
     6001 tttggtgcag aaaaacctat tctagcaggt tgctatatgt gggattatgg aggacaaaac
     6061 ccaatgccta tagatttaat gaaatatcaa cttgataaat attatgcatg gcttaagtct
     6121 aaggaaattg atggtattat tttttgttct aactgcatag ctgatctagg ccttgaagct
     6181 gtggatatca ctcgtgaatg gattgaaaaa gtcggagggg agctacttta agatagagaa
     6241 tagatatatt atgtctataa ttttgagagg gtgctaatat ttatatgaca ggataaacaa
     6301 ccctggagag taaaatttag tgtaatattc tttactcttc agggttgact tttatttagg
     6361 tctatgccat gaagtaaact ttttgctttt aaggagattt tcaacacgcc ttacttatcc
     6421 tggatgtgta agttttggca atttatgcta gttaacttat ttctataatc tccaggagtt
     6481 acacccatca tttttttaaa tattttgtaa aaaaatttaa tatcgttaaa cccgcagcat
     6541 ttagcaattt caactacttt tttatctgta gttcttagta gtaaacaagc ttgctcaata
     6601 cgcattttct gtaacaatat actcactggt aagcctgtga catttttaca tgttttacta
     6661 atataatcct tatgtagata catttcatta gatagatttt ctagtgttat atttttttga
     6721 taatacattt gtaaatattt caacacagta ttaactatat gaaccttttg gtatgaaata
     6781 ttgctttcgg aaacgttccc taacgttcga gttattttga gtattagctc tataacataa
     6841 gctctagcta aatcaacata gcctttttgc ttagaattgt attcaagata tatcttatta
     6901 aacaatattc ctaataaatc gtagttattt gctgaaaact gtaaatgata gactggtttt
     6961 tgttcattaa cgcaaaaatc atgaaaaaga aaagaggatt gcaaagattc aaagccttga
     7021 caacccacca tagaagggtc gagaaaatct ggcgtaaaca ttaaatcata ggctaaaaaa
     7081 ttttcctcag tgttgctatc agcacagaag gcatggggcg tatcatagtt aattacaaat
     7141 aaatcacctt tattaactgt atacaaatca tttccaacaa tatgggttcc ccgacccgaa
     7201 ataatataaa ctatttcaat aaatttatgt ttatgcatta tgcctatata ggcaggaaat
     7261 tctgtagaaa tatgtatgtg gatgttccta tctcgtttaa aaaagtcttt accagcaaaa
     7321 ataggtattg cagaatttgt atttatttgt gcgctataca ttattagaac agccccttta
     7381 ttaataaatt attttaattg ctactggctg taacaatcga atatcttttt ttgtatattg
     7441 caggcgttaa accagtcgtt ttcctaaaaa ctccaatgaa gtatgaagta ttcataaatc
     7501 ccacgtgcct gcttatttca gctacactca aatcatgttc tactaacatt ttacgtgcaa
     7561 gattaatacg aacatatgtt aagtattctt tgtagggcat gcctgttatc tcatttatta
     7621 tacgtgaaat ttgcttccca gataaattaa atttatccga aaggaaaagc agatccatat
     7681 tgtaatctcc gcaattatta ttaatatatt cgacaatttc aagccttgca ctcttttcct
     7741 tttcttctat attaaatcta atacgagtgc atatatcaat aatcagttta gtagtttctg
     7801 ccaaaaaact atctgcatcc tttaaaaaca taagtgttcc tattatttct tggggtatac
     7861 ttatcctgta attactagaa actcgtatta acatgcttac tacgtcagaa aaaatacagt
     7921 gttgaataat cggtgaaagg gggctatctt taacatctct tataaattgt tcgaaaatat
     7981 taattgtctc aatagaatcc ccattctcta accctacagt catttgatgc aaatatgaat
     8041 cattataaag aaaactattt gctatatccc tttgtccaaa ctgttggggg aagtcgtcac
     8101 atctttgctt gaaaattgca tccataattg attcctgaat tcttttaata tcatcgcata
     8161 ttaaccccac actgattttt aaattaattt tttctacttc cccgatggct tcaataaatc
     8221 cctttatttt cttggtttgt gttttttcac ttataattgt aagtactgaa aaactatata
     8281 aatgcttaaa tttgacggca taaagtatca tagtgtcatc agataaattt tctatcgtag
     8341 tcattagatg atccattttg tatgcctttt ctggatcaac ctgaatcacg aatgtgttat
     8401 aataagggca tgcaaaaggt atatctagat atctcataga ttcggatatg ttttcatcaa
     8461 tttctccgct caataaaagc tgtataaatt gtcggagaat aatcttacgt tggttttcta
     8521 atcttgtcat ttcctcagca agcttattgc ttgtcagcat acttttctcg aaagcagaat
     8581 ttaacattaa cgaaatctga gttatttcat ttttgttttt caactccata ttgaaaaatg
     8641 gttcatattg gtttactaat cttttaatag gacgataact atagtaggct acaagggctg
     8701 cgaaaataat cattatagct gttatggcaa tgatcaatgc tttgctgaaa gttacatact
     8761 tgcctaaacg atcataaata ccaaattcgg gaactttaag tgctaatgta aaattactgc
     8821 tatatgagtt taactttata tattttgttt catttgactt tgagcttgtt tgagagacat
     8881 tggaaggttt gagtaattca agagtgttgg aagatataat aagcagatta tcataataaa
     8941 tatgtatgtc accattgaag gtgcctatag tttcttttat acgcttaagt acgtttgctt
     9001 tttgtactac aaagcatagt gtagcatctc ctactagatt cattttttct gttaggtaga
     9061 taggaaatgc taatagaaat acattttcat tttccacagg gcaaattacc gtaaactcac
     9121 ttacggaatt taatttatca taaattcctt gataatctgt tatattaaca atatgttgca
     9181 tataatgttg aaacgtattg gtcgtggaag gaccatatac tgaattgcta tcatggtaga
     9241 gcaaaaagta ttcttgtgat acaggaatgt aatttttgta aagtacgaaa tccttaagta
     9301 aaataatatc tttgtacttg ttaacttcta gctggctggg cttgtaataa atagtatttt
     9361 gaattttata cgcaatttcc atcataagtt tttgttgatt ttctaaatat tcggtcgcaa
     9421 gttctagttt gtttatctgt tcccgtgttg cggcttcgtt aagttcgtta actgatattc
     9481 taaagaaaac tagcccaatc aatatacagg agactagtgc tatgcttgca taagacataa
     9541 tatattgtga ttttgtacct ataagtttca tcacccctac taaaccctcc ttcagacatt
     9601 tatatcttat ccggtcttcc agttttcacc tattcatctg catatcctct atctctcaac
     9661 tagtacttat ctacaaaact tatctgctaa gatctccgct atttcttcaa ttacaactat
     9721 ccgcaagggc attcattttc cccaaaatat ccgcaaaccc tgcataaagc attttcatag
     9781 aaatacctcc tttgagtttg tgacaataac attgtatagg atatagggtt gcgattcaag
     9841 ctgttatttt tctccacttt atttccggac tcgcgaaccc gaaaaaatgt caaaactgac
     9901 tatacttatg cgcagtcttt agttacatag tctttactcc cacaataatt acaatatttg
     9961 taaaaggtct taacaacaat tagggtctta attataaagt tttaaatcct cagattctct
    10021 atctttttcc gtgatgacag ttatctcttt accattggat gcaaataata atataccatc
    10081 tagtcctgtt atatatatat tgctacatac ttttttaatc ctagaatata cagaaaaagg
    10141 tggtgtagag cactgaggaa tccatgccat ttttggcgaa aaggctttta aaaacacact
    10201 agatgatgaa gagtgatacc cgtggtgggc tacttgtaat agatccacct caaattgctc
    10261 cccgtacttc tctgctaatc gaaactctgt catggtttgg atatcgccag taaataggaa
    10321 aatatgatct ccataggtta tttttaaaac caaagaaagt tcgttgttta agtgctgtat
    10381 caagtaatcg ttatcagaaa actcaacatc atccctgttt tcaggattaa gtacttccac
    10441 ttttacaccc tcacctagga aaaaagagtc ctcttccttt aacacatgaa gttgcatatt
    10501 tcttttttgc agtgcttcta gcaatgagct agaagatcca tacctagggt ctgtatattt
    10561 tgttgtcatc accttaccta caggcattgt ttctagagca tatggaatgc ctcccacgtg
    10621 atctgagtgc atatgagtag ctacaatata atctaatttt gtaactccta ttttttttat
    10681 gtcctccata atactgggga aatccgattt ataaccagca tcaatcaaca ttgtagtacc
    10741 gtcaggggca ataagtaaag agcaatcacc tgtaattata tttccatcct tttcctcatg
    10801 ttttacatta aatgctctga ttgtaaactg ttctgcatat ttattcggat ctaaaacctc
    10861 cgatagtctt gatgttggta aggttccatt gttttccagt gtttctggtg tatcagggct
    10921 tttgtcctgt gtttttttct gtatagattg taatccctct gaagaatttg tacaactaac
    10981 aagtaatgaa acaatacaat aaacggttaa gactaagatt acaaattttt tgcaattata
    11041 tttaaacatc ctacacctcc gaactatctt tagctatttg tcgaatttaa taaatagttt
    11101 ccaatattaa tagcatgaat agcaatctca tctaccaatt catcaggtag tctttgaaca
    11161 tgaccttgaa tttcataggt aaaattccca tcatacttga tctctataag ggtcttcata
    11221 atactatccc actttatatt gccatagaat gggatcaaat gctcatcatc tttattatga
    11281 ttatcgttaa tatgcaaagc ctttagacgg ctacctataa ctttcagact aatagtctgt
    11341 tcataataca ttaaattcgc atgacctgtg tcccaacaaa cccctacgtt ttcgctatta
    11401 aaataatcga ctagatcaca taattcttca ggatctgatc catatcttct tactatttta
    11461 tgatttggaa gatctgccat attttctatg gctattccaa cattctgctt ttcagcaaat
    11521 tctatccatg gtgcaaagta ttctatattc cgctgttttg aaatgctctg gctgcaatta
    11581 gaatcaacgg cagtaccagg atgcatcact gcccatttta ctccaagcat tccagatgcc
    11641 ataatactac gccttaccat ttcttccatc cactccccgt tcggattctc gaaacaaaat
    11701 ggttccagcg caccatagaa tggtatatgt gattgagtat attcaagaga caattcctct
    11761 tttatttcat tcattttatt tactgtttct tcccaattat tatttcttaa aaaaccaaaa
    11821 tctcctttat aagggcaaaa attaatatca attacatcaa atccagcttt gtaacatcgg
    11881 gttatcctat caagtatgga taatagctct ccatttttca tcactgaaga aaacaggtta
    11941 gtagatgttg ataatttcat aatcattagt tcctccttaa aaaatcaaag ttttattgtt
    12001 ataggctact taaatttctt tattattata atttttttaa ccttttaaag aacctatcat
    12061 aacgcctttt acaaaatgct tctgaacaaa tgggtaggca acgagtaaag gagcagatgc
    12121 tataataatc aaagagtact tcatagcttc tgccatataa gcctggcgcg ctaccttagc
    12181 aacatattca tcatctacaa actgtgtgga gaccgtatca attgcatcca aagtgctttt
    12241 attctgtatt aaaatcgatc ttaaaaccat ttgtagggga taataatcac tttttgatac
    12301 atatattagg gcagtgtagt aatcattcca acgcccaacc ccatagaata atgtaataac
    12361 agctataatc ggtgcagaaa ggggcagagc aatttggaaa aactgacgaa aatctgaagc
    12421 cccatcaata taagctgctt catatagttc atctggtata gttgtctgat agaagattct
    12481 tgtaactatc gtattatata tactaatgcc acctagtaaa gggatagtat acggcttatt
    12541 gagaaggtta agatccctca caagtaaata tgttggtata agtccgccac caaaatacat
    12601 tgtaaataaa aagtaccaag aaataaaaga gcgtccaggt aatttcttct tactcaatga
    12661 atatgccgta gggattgtaa gcagaagatt taatccagta cccactaggg tataaaaaat
    12721 agtattcata taccctaacc atatttcttt atgtttgaaa gtatatttat acgcatcaaa
    12781 ggaaagccct ttaatccaga atgtaacttt tccatttgct acagcataag gatcggatag
    12841 agatgctata attacaaaat aaagtgggta aaccattact atgatgatta aggctacaaa
    12901 agtaatattt atgctgtcaa atataatatc cgagcttgtc tttttcatct taatcctcca
    12961 aattttatta ataatcttta caagtataat tgttctgtta aactccccaa aactacatca
    13021 ccataagcta atggaagaag cttttttgac tataagatta actataatta aaagagataa
    13081 atttatagca gtattaaaaa gtcctattgc agcggaataa ctaaattgac cgctaataag
    13141 acctacttgg tatacatatg tggatataac ctgggatacc gataggttta acggattttg
    13201 gagcaagtat attttatgga acccaacaga taatatactt ccaaagttca taattagcaa
    13261 aatcatcact gtaggcataa tatgcggaat attaatatgt ctaatgatat ggaaacgatt
    13321 agcaccatct atgcgtgcag cttccactag ctcttgctga atacctgcca gcgtagctat
    13381 atagataata gttccccaac caattccctg ccatacacct gaccacacgt aaatactccg
    13441 aaaacattga ggttttgaca taaagggtat ggctgtacca ccaagggctt taattaatgt
    13501 atttacaata ccactgttac ggtctaaaaa gatatgtatc ataccacata acacaacagt
    13561 agatatgaaa tggggagcgt aagtaatcat ttgaacaaag cgtttaaatt ttgctttctg
    13621 caattcattg attgaaagag caagaattat ggagcaaggg aatgtactta tgctatatat
    13681 actaatactc atcgtattct ttatatagag ccaaaagttt ggaaaagtaa caaagcgttt
    13741 aaagttttta aacccaaccc atttgctccc ccatatacca atgcttgtgc ggtaatcttt
    13801 aaaagcgatt tgtactccat acataggtat ataggaaaat atgaatacat atataatagc
    13861 tggtagcaat agtgcataca attgccagta tgataatatt actttcaaat tatttgaaaa
    13921 cttgccttgt atatttctca tgatttatcc tcttcatttc tttcatcaat tttaataaac
    13981 ttttggtatg ccctagatat agtaatcaag aaaattcgtt tcgctcatga gttaaaacaa
    14041 tgagacgaat tttcttgttc gctagggggg actactcccc ccttcgacgg atgttaggat
    14101 cctagtgcat cctgagcacc aaagcgtttt gcgaagcaaa atcgcctgct cgctctacaa
    14161 tgcccatcta ccctccaaat cggaaagggg aatccccttt ccaagcccca gtatagtaat
    14221 tttctaggag atttataaat tactatactc taaaaaatat gatgaagggc tataaatatg
    14281 gcattccaaa atattattat ttcaagtttc ttactaggct acttaaatga ctatttcttt
    14341 gtttgtttta tttttgtttt tcgagatatg attggtactc atcaatatag tactgctcat
    14401 attcatccat tcccagagat ttcaattcct taaggtattg ttcaaattcg ttaagtgaac
    14461 gtgtgccagt tataaataat gcaacttgct ctgtaacata aggcccgatt acggcgctat
    14521 actcagcaag catttccaaa tcttcctcac tcctataaac aattggagga tatgcaggtt
    14581 tggcataagg gtagaacatt ttaactctgc cataagtatt ccagttacca ccctggttta
    14641 aatctaaagt tgatatgtcg agaccttttt tatattcttt accagcatat ttcgccattt
    14701 tttcgcctct ccacgaatgc aatccaaatt gcaagctata tggcattaga atgttggaaa
    14761 tataattata agcacttggc tcatcaggat aatattcgct gtttgctgat tcatcccaaa
    14821 tccaaccttt aaagccaagt gtttcttcag taccatccat tgcgccattc cacatgaaga
    14881 aacaattatc accataatat aaatcaatga atcttgcaca aacttcagga tatttagtat
    14941 tagcagatat tgctattcct tgcatatcat atttactagt ttgaggcact tgtggggtat
    15001 cattccattt actagttaaa ggttttatgt aaaaccattc ctgatatttt tttggttcag
    15061 taagtaatac atgcattggt tggcacatat aaccataaac tccttctact gccatagcat
    15121 taactttggt ctgatccatt gtgaaaaaat caggcgaaat aagttcttca tcgtatagtg
    15181 tttttgcaat agagagaaaa tcgtaaaaaa tatcatcatt acatggaaat gttgctttac
    15241 cgtctcttat tgcaattgta agcccattac ctttagcggc tgacgcccat gaataaggaa
    15301 agttataccc catggcattt attattgtgg ctaacgggtt taaatagttt aaacttccac
    15361 ctaaaggata tacattctca gagccatggc catcaacatc ttctttctta aaaatcctca
    15421 ttgtttctat gaattcatcc aaggtggagg gtgattcaag attaagtttg tccagccatg
    15481 attgtctaat atttaccatt tcaactgttc cttcatcgtc aagtatcctt ggtattttgt
    15541 atacattccc atctggggca gttgcacttg ccataagccc tggatcttcc ttgcttagtt
    15601 tgtatacatt aggcatatag gtagggttga tataatcatt taatgataaa agttgacctt
    15661 cattaacccc gtattttaca atttctcttg tattaatacc gaaacctaaa acaatatcag
    15721 gaacatcttt ggatgcaaac attaaattta accgttcact atgtgcttcg actggcactg
    15781 cttcaatttt ggggtctatg tttaagaatt tttcgataaa tttgaagaac caacaatttt
    15841 caacactagc atataatgcc ggatttgtta ataaacagaa cctaaggctc actttatggc
    15901 cttccttaac tattggtatt tcgctagcat aatttatatg ctcaagtttt ccatctaatg
    15961 catctggtgt agttgtagaa tcattaccat tagatgctgt ttttgtatca gatttgattt
    16021 ctttattgac cgtacagccg ctaattatgc ttgtcaacat gattattact aaggttagcg
    16081 ctattagtgt tcttttcata aattattcat cctttctatg tacattgtta atttactttg
    16141 acttcaaata gatcctaggt agtacaagtt gcgtgaattt tgattgtgat taatagaaat
    16201 ttgtaagctt acatttcacg catattataa accctgcaaa ttatatgttc aatgacaggt
    16261 ttaggcgtcg ataatataaa acggacattt tgttgtttaa atgcccaaat gtccgtttta
    16321 tattatcaaa cttttttgtg ctattttaaa tgattagctt tatgatatgc caacattttt
    16381 ttaacatatt atcaccgaga gctcaataaa cccccaaaag tatctattct atttcctttg
    16441 tggcattcca agtattgaaa acaatgaagt ttccgtaagc caaatgtgta aaaatacaag
    16501 tattccaact ggacagcctc accacccact agatcaagat ctcgatatat tagaacagga
    16561 gaaaacagtt ttctgtttga aaaatatact aatgagcgca aaagattatc atgaggcata
    16621 cccaaacggc catgtccaag ataatgctcc agatgacacg gcacttcacg cagataaacg
    16681 ccacgccgga gctttttccc gtcccgccgc aggcagtgca ggcatcagag ccggaataga
    16741 ccttttcgcc gatttttacg ctgctgtccg aatgatagac ggcatagttt aaataataaa
    16801 ttaaatacaa taaccataaa tatttaaaat cattatacaa gtatagaaat gaattatcaa
    16861 tgacattttg tcaatgacta taatgctgtt gacaaaattt ttcgttaagg taattaatat
    16921 taacatcacc cataatgttg gtttgataat tcaagtaaaa aacgccgcag agcttcatta
    16981 ctcttcagca tttcatatta tatttgaaac cttgaaaagc ttcgcaccca ctttgacagc
    17041 aaatgccgtc tgaagttggt gcaaatactt tatttataat ttatgtgttc tataaaatag
    17101 atcaatttgt acctacatcc aacctgacca aacaaaccct gcgacatccg aaaacatcaa
    17161 aattaaccac atgttaaccg ctgacatcta acctgaccac acgccaaact atgacatcta
    17221 aatcactctg tcgcactgaa aattaccagc tttgacttat tcccacgaac cgataaaatc
    17281 acgattttca tataagggtt ttcaaactca agtgcactta aagccttgat atataaggct
    17341 tttccataca ctcacccctt ttcccttgac atcaatatct cgcactccac atgccttgaa
    17401 gatacaaaaa agatgccgtt agaagctcat tcctttaact caggtactcc agccttttta
    17461 caagtcgaat caagtaccca t
//
LOCUS       DAOSDD010000080        17299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1343_length_17299_cov_4.837973, whole genome shotgun sequence.
ACCESSION   DAOSDD010000080 DAOSDD010000000
VERSION     DAOSDD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17299
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_17299_cov_4.837973"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..3697)
                     /locus_tag="PK733_08805"
     CDS             complement(293..3697)
                     /locus_tag="PK733_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10677.1"
                     /translation="MTTLKPRVLNYETKISKSEKITIIAPSSEYGIKGAHQIFNKIKV
                     YSNADIADILVNPDKEILYDARGHVILIGNLSDSKCLKELYYKQLAVTDLHYPGLEGY
                     EIRTLINPLGTGYNIIQIGYSDEKGLSKAIDIFCSEITLSSDSNSKTMPSSNLDATAF
                     SIRYFNDLKPTRLHFPEGKVQELLNSSYSLDDPTIHYTAPIDEVGYLAYLTGNKKLLD
                     LYYNSWKVLLKLEYGHLKLHKRVVCWRLHEITGMVPEEIRSDIVNFFYGWADGDEGVG
                     SILRNIYQMPDFPRQNHGLIPSMGLVLLHDYFTKYYPELQRPAVWRETADRVHSVYFN
                     GNWKPLCDGLCHGWWLSQPAMLEYGLFDDEHKYFENGGAGNAALCAMAVVNNEGWLPN
                     SGDSDIIRQFPGYVLDMTSEYYRNGKAKYISNLAPIWRRGYSGPISFLPRMYDIGLKP
                     EEPEELIGVTVIPIDPNVYNAWRDYPDIAHMVADTPPVAPIEKCFDKLAVRTGFNRKD
                     EFLLIDGLGGGSHSYADAMGILDYEKYGVSFVVSEDQLYWIEPENHSMVTMYRDGKAN
                     KIPSFAEIDKIEEISGDKIYISMILKNKEEADWRREIYFIPDKFVMINDTIIANEEGD
                     YSLETHFRTPGYVEINGNNFQSRKQTRYGDNVEFLLQINCSHQLNCSVKEIPLNYSYR
                     ALPGEGKPISIETDPLEYAKIRYHLQDDNIVLSACTGKTSIYMNKGDKVTFTSLLFAS
                     DEAKEVSIKEINGKFNVKIENEIYPTYFDSYSIQSAEKQKDGTLSATATELFKSHSDI
                     AAINVFDSKIWCGLSNGDIISVDKSGNIRGIASLNTQIHDFTLHNGFIYTGCGTNSII
                     KMTDKGDLVWNIETVRIPTIYPWWELDYPGAMSLKIIKYKDKDTLIAGCGDNNIRFYD
                     LDGNLLDSYYFFAAVPNVIESYDVNNDGKNEILLAGNIITCVSNVDVLDDNGKLLHKF
                     GTENWTSIATVLKCFDIHGKKTVALGVNHRSNFKLYQFNPDSKDIKGEYIINDRLAGA
                     VTGIDLDYDNGVIVAGTSQGFIAAYTVTGEQKWVKMINGIICDVKYFNKLFWIADEAG
                     YVYKYNKDGVLIDTIYVNDKVKKLENVDEVMYIITKGSIYVLNEIY"
     gene            complement(3821..6136)
                     /locus_tag="PK733_08810"
     CDS             complement(3821..6136)
                     /locus_tag="PK733_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10678.1"
                     /translation="MSGSKRSEKKLNTLFIKLICFTALITILSVMFAGMFPYIYLQNE
                     MSDDIKQFNNQKLQKLKDVIEIDLFDMTLTFLSDFVNSSNIYDIINTMEDSDGKINAY
                     SIVQMRNLLRSKITTFSYPFDDILVYHEKDRKIISAKMGLQDLNDKSHKMRSNDEWIK
                     HLDDLSPHKTIKWLDNLEVPDYSSNNFTSTMKCISIIIRLNPKLQSGSLVYICITIPE
                     IEIQRMMRKVNPDNQIQMKLVKANGDCVASTNHIEEQVQFKKPIGTGMFSGDESIYSE
                     VQDNTVYSIMKSNTNDWYYLIETPRDHYYKRLDTFNRNVILAGIAIFSLMFSVSMFVI
                     FRLTSPFGDLLNIVSGLTRTADNKCKKTEMRLVVDAFSGLVETSNLQQQLLSGNRSLV
                     RQSLLTQLLTGIKVSSAELESCIKFLNTSLKYPLYTVVLLQFIPPNNERAAGILSSLE
                     QYINENKSLDAEFICYKFDEKLYSIIINHNNIDISEIKDFFEKLLITFENSLMITGSA
                     VIGSTKETMEEIFKSYRNATDALCYAAYYPSISVLCYQETKLWDMNTQFDETLISKLT
                     KSINMMHTDNVCMYIKLICDYIHNNFISYKTCMKTVEYIINVVEMEAMSMEISLGNEE
                     TTNLANQVENCYFITDMFDCIKNFIVNMIASADKIDASGVSKEYANAAIAYMNENFNK
                     DVSVQDIADEVGVSRYHLSRIFKENTGVTLVDYLNRIRFSKAEVFLKNTDMSINEIAK
                     AVGFNNISYFNRKFKQMFGMTPSQFYGEINN"
     gene            complement(6171..9524)
                     /locus_tag="PK733_08815"
     CDS             complement(6171..9524)
                     /locus_tag="PK733_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10679.1"
                     /translation="MSYLKARKISKITSLTEASCISIFTPKDDEASDIIEDLIKAFQK
                     ETRVDVFTNPDSTELYNVRNPIILIGNLSNSFCVEAMYYRYLLVTDLTYPGIGGFEAR
                     TLLDPLATGYNIIHLGYSDISGLKKAVEALIKNISKDYIEYMCEINPTQLPMPQSRVE
                     SICADKIDFTDQTFYYTALQLEDKGYIGYLTGDSQQLEEFNIAFEMLLDAPLDHLKLF
                     KRCIIWRLLEVTGMLRDDLHEQSINFFYNWADSKEGIGSFSSQMYQSPYFPRQNHGLM
                     PAFGVYLLANYFEMYYPDLEHPAIWKETAKRVFAPYENGNWKPLCDGLCHGWWMSQPP
                     MLDYALFDDAHAYFISGGAEKAGLCAMSVINNEGWMPCAGDASPIRNFPHYTLDINAA
                     YFKDGRYRFISELAPLWRRAYNYPTLDILRRFDIGVEPIEPTELLDIIITPMDPLVYN
                     AWKYEPEIAKKAADTPPTYPIEKCFDKIAIRTGFKLEDDYLLIDGLGGGSHSYADAMA
                     ILDYEVFGISFIVGEDAIGLSDPTNENTITIYRDGYTAPVPAFPAIEEAKKIGGNYYV
                     KLKSGNYNGAIWERDYYLVPAAGLIIQDKVTAETKGMYAIESHYRCPGIAELKKDCNV
                     SKLHSKRRNINGDIVNFEISTISNVELDYTVREMSFTEYRYRNYPGSKLPINKEVDGV
                     HYLKERYHLEDNELYLNSYDAKTCISLEAGDSVVFTSLAFPTKTNTKAFLDMTGKYVI
                     LDIDGNRIETPLKAHNVFAAAQVENNVDCRMPVNVFTSNITYSGICSEGSLLCGLQDG
                     RLMNISSAGIICAATLDDEINAAEYNGEFYVVSHGKASISLLDKTGISKWTIEVKRIP
                     TLYPWWELNYPTAVRLLIGKYKGQDAIIAGCGDDHVHFYSLKGEPLGTFYHKITGVPD
                     MIEFYDVNQDGQYEILITSSMLTCTSVVDIINTEGESLHRFGGEGWKSNSTCIRCFNI
                     EDKVYIAHGIYQKCNLELRVFDKTGPNELIEGRYIIKEHVAGAVAGFAVLPEVDVIAA
                     GTSQGFVIAYNFAGERIWFNGIKGALCDTIEYKNKILAADLGGYVYILSPEGAIVDKL
                     KSGIKKPKFYLAGEELFAVGENRVVRLDF"
     gene            complement(9685..11277)
                     /locus_tag="PK733_08820"
     CDS             complement(9685..11277)
                     /locus_tag="PK733_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10680.1"
                     /translation="MKRKLALLLSMFMLVSLFAACTTNKSNSTATGGKDETTSTTSAN
                     KEERYKITVVAPTAAPYDNNSDMVKFFTENSNIDWDMQYIESAKAAEIMNLRFASGDI
                     PDIYSNLGYAKFYDLVNEGIGGTISEKLLRSTAPNIAKYFDELGDNCWTVAKHENGEL
                     FTFPGINAEYMYGSAIIWRQSWLDKFGEEIPRTLKDAERIFYKFAKEDPDGNGKDDTY
                     ALSADGMTQVFRAYGVMDIFEDENGTLQYPVTSPKYTEALNLLAKWYKDGVLDPEYIT
                     GENKGGYAALSHSFCSGRIGFTAKGINNHWRPVEWLKEYPSIAPAANQELFNQTFPEE
                     TYSYGYPLEGPYGDKSTGMTNYSYWSGFSKKFTDDIPKLTAFLKFVEWSNGFNNPEDY
                     LLVRYGIKGKHWDYDQNNFPAYNEEYRTSDERYGAGLVSVFTFTNNYHALNLVNPVYS
                     TWRDKLYAEPGRSVMNKSYFVRSLPSAAEFSELSKLEGEFKVDVITGKRSVSEYDKFI
                     AEWRKLGGDKMTKEANEIRIDK"
     gene            complement(11438..12337)
                     /locus_tag="PK733_08825"
     CDS             complement(11438..12337)
                     /locus_tag="PK733_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10681.1"
                     /translation="MAKIGLQKNLKNEGIGSMVFDFTNNIVIFLFCLTIIIPFWYLFV
                     QSVDNLASPSGSIYLWPQQFTLENYKMVIRNKYIGIAYLNTIFRTVAGTTLSLFFTSM
                     GAYAISKKRFPNRVFWSIFIIVTMFFSGGLIPTYLWISKLKLINSRAVLIIPGIIGAY
                     NLVLIRNFFSQIPGELEESARLDGSGDFNTFIKIILPVSKPILATIALWLAVGHWNSW
                     FDCMIYIRDTKKIVLQVVLQRIIIQGTTKEIIEGSEQMLFDAKPEMIKAACIYVATLP
                     ILCTYPFLQKYFVKGIYVGSLKG"
     gene            complement(12337..13290)
                     /locus_tag="PK733_08830"
     CDS             complement(12337..13290)
                     /locus_tag="PK733_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10682.1"
                     /translation="MEKTLKNKSKPDINKCTLIFQNYAKEWQLVFMFLPVVLCLGIFC
                     YAPMYGIVIAFKDYRIIDGIMGSPWVGLKHFQSLFTAPSFRQVFGNTIKISFLRMLFG
                     FPAPIILSLLLNEIKNVHYKKIVQTVSYLPHFLSWVILSGIFINFLSPTTGFVNQVIS
                     YYGGKPIYFVADIDYFVPMLIVTGIWKGVGWGTIIYLAAISNINPELYEAAIVDGANR
                     FKRIIHITLPGIAPVVSVMFILNSSNLINAGFDQIFNFYNEAVYRVSDIIDTYVYRRG
                     IQQAQYSFSTAVGLFKNIISFSLVLTTNLIVKRVNKEGGIW"
     gene            complement(13572..14588)
                     /locus_tag="PK733_08835"
     CDS             complement(13572..14588)
                     /locus_tag="PK733_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOJ10683.1"
                     /translation="MYSFMNDYSEGAHPRILERLIKYNFEQNTGYGLDVHSEKANEYI
                     KKQIEKEDADIHFIPGGTQTNLLVISSFLRPHQCVIAASTGHINVHETGAIEATGHKV
                     FTMPCTDGKLTPADIQKALDYHCDEHMVQPKMVYISNSTELGTIYSKAELSAIHHICR
                     SNDLILYLDGARIGSALTCKANDLQLSDIARLVDVFYIGGTKNGALLGEALVICREEL
                     KKDFRYLIKQRGAMMAKGFVIGMQFEVLFQDNLFFELAIHANRMAEKIAAILKDYGCS
                     FYAPPCTNQIFPILPNDVLEKLAQRVEFSVQTKIDEERSAVRFVTSWATTEESVEELS
                     KFFR"
     gene            complement(14810..14886)
                     /locus_tag="PK733_08840"
     tRNA            complement(14810..14886)
                     /locus_tag="PK733_08840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14850..14852),aa:His,seq:gtg)
     gene            complement(15004..17088)
                     /locus_tag="PK733_08845"
     CDS             complement(15004..17088)
                     /locus_tag="PK733_08845"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ10684.1"
                     /translation="MLKYGFLRIAAAVPVLKVADCSYNTDKIIGIIREAYSEGAQFIL
                     FPELSITAYTCGDLFLQEALLREANYKLAEILSQTADMDIISIVGLPLRLQNRLFSCG
                     TVLQKGRILGVVPKCCIRNGKGYNEGRWFSTGKNILNCNINICNQEVPFGTNLLFSAQ
                     FNTRYDTEPNFEPNFEPNFEPNLQPNLQTLAVPFALELGGDLQMTLPQSSFHSTAGAL
                     IIFNLSAAFEETGLYEYKKDLVRLQSARCNTAYIYASAGVNESTTDGVFGGHSIISEN
                     GTILGEGERFRSESQIIYADIDVEKLISEKIKNSCFMEDNMCLSVDYKNYSTSTSAFR
                     QYRNICFPIDDIIIDDAIGKSSPKKLYRHIDPYPFIPADESIRAARCLEIFEIQTWGL
                     VKRLEYTGSKYAVIGISGGLDSTLALLVTANAFDKLGLPRKNIIAVTMPGFGTTKTTH
                     KSSLQLMEAVGASIREIDIKDACLQHFRDIGHDVNQLDTTYENVQARERTQILMDIAN
                     KEGGLVIGTGDLSELALGWCTYNADQMSMYSVNCGIPKTLIKFMIYWAADNIVDESTK
                     DILYRIIDTPITPELLPPDPKGEIRQKTEDIVGPYELHDFFIYYVVRYGASPRKVLFL
                     AQQAFSEKYSGSEIKKWLEVFYKRFFSQQFKRSCMPDGPKVGTISFSPRGGWSMPSDA
                     QRTLWLRQLEEI"
     gene            complement(17163..>17299)
                     /locus_tag="PK733_08850"
     CDS             complement(17163..>17299)
                     /locus_tag="PK733_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOJ10685.1"
                     /translation="EEAGTIEDVYEPYLLQLGFINKTTRGRVATRRAYEHLGMKYADE
                     "
BASE COUNT         5367 a         3471 c         2630 g         5831 t
ORIGIN      
        1 aaagaacatc ctcctatatt agcctttcct aatataagct caacatcctt acctgatgct
       61 gcaatctctt ctaaatatct tgttgcatta tcagctaaac tatttcctat agtaagtact
      121 tttatcattc ataacatctc cttagaaatt atttaactat tgggtgtatt taatttatat
      181 gaagaaaata ttggtaagga gtattggttg aagtgctgca gtttggtata gatataagca
      241 ctgtaacgat agcttctgga atatatttct tcataaattc aaaatgtgca ctttaatata
      301 tttcatttaa cacatagata cttccttttg ttataatata cataacttca tcaacatttt
      361 ccagcttctt aaccttatca ttgacataga ttgtatctat caatacacca tctttattgt
      421 atttgtaaac atacccggct tcatctgcaa tccagaacaa tttgttgaaa tattttacat
      481 cgcatattat tccattaatc atttttaccc atttttgctc tcctgttaca gtgtaagcag
      541 ctataaatcc ttgacttgtg cctgcaacaa taacaccatt atcatagtct aagtctattc
      601 ctgtaacagc ccctgctaac ctgtcattaa taatgtactc acctttaata tccttgctat
      661 ctgggttgaa ttgatataat ttgaaattgc ttctatgatt aacccccagt gccacggtct
      721 ttttaccatg tatatcaaaa cacttaagta ctgtagcaat gctagtccag ttttcagttc
      781 cgaacttatg caatagcttt ccattatcat caagtacatc aacattagaa acacatgtta
      841 taatattacc ggcaaggagt atttcatttt ttccgtcatt attaacatca tatgactcaa
      901 taacattagg cactgctgca aagaaataat aagaatctaa taagtttcca tcaagatcat
      961 aaaacctgat gttattatct ccacaccctg ctatcaaggt atctttatct ttatatttta
     1021 taattttcaa actcattgca ccagggtaat ccaattccca ccaaggatat atagtaggta
     1081 tacgaactgt ttctatattc catactaaat ctcctttatc tgtcatttta ataatactgt
     1141 tagtcccgca tcctgtatat ataaatccat tatgcaaggt aaaatcatgt atctgtgtat
     1201 ttaaagatgc aattcccctt atatttccag acttatccac actgattata tctccatttg
     1261 aaagtccaca ccatattttg ctatcaaaaa cattaatggc cgctatatca gaatgagatt
     1321 taaataattc tgtcgcagtg gcagataagg ttccatcctt ttgtttttca gctgactgta
     1381 ttgagtaact atcaaaatat gttgggtata tttcattttc tattttaaca ttgaacttgc
     1441 catttatttc tttaattgat acttcttttg cttcatctga tgcaaataac aaacttgtaa
     1501 aagttacttt atctccttta ttcatataaa tggatgtttt gccggtgcaa gcactcaata
     1561 caatattatc atcttgcaaa tgatatctta tttttgcata ttctaaggga tcagtttcta
     1621 ttgatattgg ttttccctca cccggtaagg ctctgtatga ataattaagg ggaatttctt
     1681 ttacagagca gtttagctgg tgagaacaat taatttgaag aagaaattct acattatctc
     1741 catatcttgt ttgcttcctg ctctgaaagt tattaccgtt tatttctaca tatcccggtg
     1801 ttctaaaatg tgtttctaat gaataatccc cttcttcgtt ggcaattatt gtgtcattta
     1861 tcataacaaa cttatcaggt ataaaatata tttcacgacg ccaatcagcc tcttctttgt
     1921 ttttcaatat cattgatata tatattttat caccgcttat ttcttctatt ttatcaattt
     1981 ctgcaaaaga aggtattttg tttgccttgc catctctata cattgtaacc atagaatgat
     2041 tttccggttc tatccaataa agctgatctt cagaaacaac aaaacttact ccgtacttct
     2101 cataatccaa aattcccatg gcatcagcat atgagtgaga accaccgcct aatccatcaa
     2161 tcaataaaaa ttcatctttc ctgttaaacc ctgttcttac agcaagtttg tcaaaacatt
     2221 tttcaatggg ggcaacagga ggtgtatctg caaccatatg agcaatatcc ggatagtctc
     2281 tccaagcatt atatacattg gggtctattg gaataacagt gactcctatt aattcttcag
     2341 gttcttcagg ttttaatcca atatcataca tccttggcaa aaatgaaata ggaccgctgt
     2401 aacctcttct ccatataggt gctaaattgc ttatatactt tgctttccca ttcctataat
     2461 attcagatgt catatccagt acatacccgg ggaattggcg aattatatca ctatctcctg
     2521 aatttggcag ccacccttcg ttatttacaa ctgccatagc acaaagtgca gcatttccgg
     2581 caccaccatt ttcaaaatat ttatgttcat catcgaacaa tccatattca agcattgcgg
     2641 gttgagatag ccaccagcca tgacacaatc cgtcacataa cggtttccag tttccattaa
     2701 agtaaactga gtgaactcta tctgcagttt ctctccatac agccggtctt tgtaattcag
     2761 gataatattt cgtaaagtaa tcatgtaata aaactaaccc catagaaggt attagaccat
     2821 gattttgtct ggggaaatcc ggcatctggt aaatattacg taatatggat cctacccctt
     2881 cgtcaccgtc cgcccaccca tagaaaaagt taacaatatc acttctaatt tcttcaggta
     2941 ccatccctgt aatttcatga agcctccagc acacaactct cttatgaagc tttaaatgac
     3001 catactcaag tttcaatagt actttccatg aattgtaata taggtccaga agttttttat
     3061 ttcctgttaa atatgctaaa taacctactt catctatagg agcagtataa tgtattgtcg
     3121 gatcatctaa agagtaagaa ctgtttaata attcctgaac tttgccttcc gggaaatgca
     3181 agcgggtggg tttaagatca ttaaaatatc taatggaaaa agccgttgca tctaaattgg
     3241 aggaaggcat tgtttttgaa ttcgaatcag aagaaagggt tatttctgag caaaagatat
     3301 caattgcttt tgaaagacct ttttcatctg aatatcctat ttgaattata ttgtatcccg
     3361 taccaagtgg atttatgagt gtacggattt catatccttc caatccaggg taatgcaaat
     3421 ctgtaacggc taattgttta taataaagtt cctttagaca cttactgtca gataaattac
     3481 caataagtat tacatgacct ctggcatcat ataaaatttc tttatccggg ttaactaata
     3541 tatctgctat atctgcattt gaatagactt ttatcttatt gaaaatttga tgtgcacctt
     3601 ttatgccata ttcacttgaa ggtgcaataa ttgttatttt ttctgatttt gatattttgg
     3661 tttcgtaatt caatactcta ggttttagtg ttgtcatagt aagtctcctt ctcatcaagg
     3721 gaacacttct tcaaggtgac actcttttct cgagtaatga tatttctttt acgttaaaga
     3781 gcgtatcctt gcgatgttaa agtgcgtccc tctacaattt tcagttatta atttctccat
     3841 agaattgtga tggcgtcatg ccaaacatct gtttaaattt acggttgaaa taagatatat
     3901 tattaaaacc aacagcctta gcaatctcat taatgctcat atcagtattt ttcaagaaca
     3961 cttctgcttt actaaaacgt attctattta gataatcaac taaggtaact cctgtatttt
     4021 ctttgaatat tcgtgaaaga tgatacctgc ttactcctac ttcatcagca atatcttgaa
     4081 ctgatacatc tttattaaag ttctcattca tataagcaat tgctgcattt gcgtattctt
     4141 tagacactcc tgatgcatca attttatcag cagacgcaat catgttaaca ataaagtttt
     4201 taatacagtc gaacatatcg gtaataaaat aacagttttc aacctgattg gctaaattag
     4261 tagtttcttc gttacctaaa ctaatttcca tagacatagc ctccatctct acaacattga
     4321 tgatatattc aacagtcttc atacaggttt tataggagat aaaattattg tgtatatagt
     4381 cacatattaa ttttatgtac atgcaaacgt tatctgtatg catcatgtta atactctttg
     4441 tcagctttga aattaatgtc tcatcaaatt gagtattcat atcccatagt ttagtttcct
     4501 gataacacaa tacgcttata cttggataat atgcagcata acaaagtgca tctgtggcgt
     4561 ttcggtacga tttgaaaatt tcttccattg tttctttggt tgaaccaata actgcagatc
     4621 ctgtaatcat aagactattc tcaaaagtga ttaaaagttt ctcaaaaaag tcctttattt
     4681 ctgagatatc tatgttatta tggtttataa tgatggaata taacttttca tcgaatttat
     4741 aacaaatgaa ttctgcatct aagcttttat tctcatttat gtattgctca agagatgata
     4801 aaataccagc tgctctttca ttattcggtg gtataaattg caatagtacc actgtatata
     4861 aaggatattt caaagatgta ttcagaaatt ttatacatga ttcaagttca gcagagctaa
     4921 ccttgatacc ggtaagcaat tgtgttaata gactttgacg gacaagggac cgatttccgg
     4981 atagaagctg ctgctgcaaa ttacttgttt ctacaagtcc gctaaaagca tctacaacta
     5041 accgcatttc tgtttttttg cacttattat ctgcagtccg cgtcaaacca gaaacaatat
     5101 taagcaaatc tccaaatggc gatgtcaatc tgaaaataac aaacatgctt actgaaaaca
     5161 ttagcgaaaa gattgcgatg cccgcaagta taacatttct attaaatgta tctaaacgtt
     5221 tatagtaatg atccctgggt gtctcaataa gataatacca atcattagta ttggatttca
     5281 taatagaata tactgtatta tcctgcactt cagaatagat tgattcatcc ccggaaaaca
     5341 tgcccgttcc tataggtttt ttgaattgta cctgctcctc tatatggtta gtagatgcaa
     5401 cacaatcacc attggctttt acaagtttca tttgaatttg attatccgga ttaaccttcc
     5461 tcatcatcct ttgaatctct atctccggta ttgttataca tatatacacc aaactgccgg
     5521 attgtagttt gggattaagc cgtataataa ttgaaataca tttcattgta cttgtaaaat
     5581 tattactgct ataatcgggc acttccagat tatcaagcca cttaatagtc ttatgaggag
     5641 atagatcgtc cagatgctta atccactcat cattgcttcg cattttatgg cttttatcgt
     5701 tcaagtcttg taagcccatc tttgccgaga taatctttct gtctttttca tggtatacaa
     5761 gaatatcatc aaaagggtaa gaaaatgttg ttattttcga acgtaaaaga ttccgcatct
     5821 gtacaatcga gtatgcattg attttgccat cactatcctc catggtgtta ataatatcgt
     5881 aaatattaga cgaattaaca aaatcgctga gaaatgtcaa tgtcatatca aatagatcaa
     5941 tttctattac atccttaagt ttttgtagtt tttggttgtt aaactgtttt atatcatcac
     6001 tcatttcatt ttgaagataa atatatggaa acattccggc aaacataaca gataatatgg
     6061 taattaaggc tgtaaaacat atcagtttta taaaaagtgt atttagtttc ttttcacttc
     6121 ttttactacc agacatattt aacctccatg ttttcttctt acctgtgtcc tcaaaaatct
     6181 aatcttacta ctctattttc gcccacagca aacaactctt cacctgccaa ataaaattta
     6241 ggtttcttaa tcccgctttt tagtttatct acaattgctc cttctggtga aagaatatat
     6301 acataccccc ccaagtccgc tgcaagtatt ttatttttgt actcaattgt atcgcataat
     6361 gctcctttaa tgccgttaaa ccatatacgt tctcctgcaa aattgtaggc aataacaaaa
     6421 ccttgactag tgcctgcagc aattacatct acttccggta gcacagcaaa acccgcaaca
     6481 gctcctgcaa catgctcctt aataatatat cgtccttcaa ttaattcatt tggaccggtt
     6541 ttatcaaata ctctcagctc caaattacat ttttggtaaa taccatgagc tatatatact
     6601 ttatcctcaa tattgaagca tcttatacat gtagaattgg atttccagcc ttctcctcca
     6661 aatctatgca agctttcacc ttctgtattt ataatatcca caacagatgt acatgtcaac
     6721 attgagctgg taattagaat ttcatattgc ccatcttgat ttacatcata aaattcaatc
     6781 atatccggaa caccggtaat cttatgataa aacgtgccta aaggctctcc ttttaaacta
     6841 taaaaatgta catgatcatc tccacaccct gcaattattg catcctgccc cttatattta
     6901 cctattaaca atcttactgc tgtaggataa ttgagctccc accaagggta tagtgtgggt
     6961 atacgtttaa cctcaattgt ccatttggat atacctgttt tatccagcaa gctgatagag
     7021 gctttaccgt ggctaaccac ataaaattcc ccattatatt cagctgcatt aatttcatca
     7081 tcaagagttg ctgcacatat tatacctgca ctacttatat tcataagcct gccatcctgt
     7141 aagccgcaca atagcgaacc ctcagaacat atgcctgaat atgtaatatt acttgtaaat
     7201 acatttacag gcattctgca atctacattg ttctcaacct gtgctgctgc aaaaacatta
     7261 tgggctttta gtggtgtttc tattcgatta ccatcaatat ctagaattac atacttgcct
     7321 gtcatatcaa gaaaagcctt tgtatttgtc tttgttggaa aggcaaggct cgtgaacact
     7381 acactgtcac cagcttccag agatatgcaa gttttagcat cgtaagaatt caaatacagc
     7441 tcattatcct ctaaatgata gcgttcctta agataatgaa caccgtccac ctctttattt
     7501 atcggcaatt tggatccggg gtaattacgg tatctatact ctgtaaatga catttcacgt
     7561 actgtgtaat ctaattctac attagatata gtagatattt caaaattaac tatatcacca
     7621 tttatattac ggcgcttaga atgtaatttg cttacattgc agtctttttt aagctctgct
     7681 atacccgggc agcgataatg agattctatt gcatacatac cctttgtttc agcagtgact
     7741 ttatcttgaa ttatcagacc ggcagccggt acaagataat agtcgcgttc ccaaatggct
     7801 ccattgtaat tgccggattt tagttttaca taatagttac caccaatttt ttttgcctct
     7861 tcaatagcag gaaatgcagg aacgggtgca gtatagccat ctcgatatat agttattgta
     7921 ttctcatttg tagggtcgct aagtcctata gcatcctctc caacaatgaa acttattcca
     7981 aacacttcat aatcaagaat agccattgca tctgcatatg aatgtgaacc gcctccaagc
     8041 ccatctatca atagataatc gtcttctagc ttaaaacctg ttcttattgc tattttatca
     8101 aaacatttct ctattggata agttggtggt gtgtctgcag ctttctttgc tatttccggt
     8161 tcatatttcc atgcattata gactaatgga tccataggtg taataataat atcaagaagc
     8221 tcagtaggct ctatcggttc tactccaatg tcaaatcgtc gtaaaatgtc aagagtagga
     8281 taattgtatg ctcttctcca taatggtgca agctcgctta tgaagcgata acgtccatct
     8341 ttaaagtaag cagcattaat atccagtgta taatgtggaa aatttcttat gggacttgca
     8401 tcacctgcac aaggcatcca cccttcatta tttattacac tcatggcaca aaggccggct
     8461 ttttccgcgc caccggaaat gaaataagca tgagcatcat caaataatgc ataatcaagc
     8521 attggaggct gtgacatcca ccagccatgg cacaatccat cacataatgg tttccagttg
     8581 ccattttcat aaggtgcaaa tactcgcttt gcagtttctt tccagattgc agggtgttct
     8641 aaatccggat aatacatttc aaagtaattt gctaataaat atactccaaa agcaggcatt
     8701 agaccatgat tttgtctggg gaaatacgga gactgataca tttgacttga gaaagaacca
     8761 ataccttctt tactatctgc ccaattatag aagaaattaa tactttgttc atgtaaatca
     8821 tcccttaaca ttcctgtaac ttctaaaaga cgccaaatta tacatctttt aaataacttt
     8881 aaatggtcaa gaggcgcatc taacaacatt tcaaaagcaa tattgaattc ctcaagctgc
     8941 tgactgtctc ccgtgagata gccgatatat cctttatcct ctaattgtaa tgctgtatag
     9001 taaaatgttt gatctgtaaa atcaatttta tctgcacaaa tagactcaac tcttgattgt
     9061 ggcatcggaa gctgtgtagg atttatttca cacatatatt cgatataatc tttacttata
     9121 ttttttatca atgcctctac agcttttttt agtcctgaaa tatcagaata acctaaatga
     9181 attatgttat accctgtcgc caaaggatcc agcaaagtcc ttgcttcaaa gccgccaata
     9241 cctggatatg ttaaatctgt aacaagaaga tatctatagt acatagcttc aacacagaaa
     9301 ctattagata agttgccaat aagtatgatt gggtttcgta cattatataa ttcagtagaa
     9361 tcaggatttg taaatacatc tactctggtt tctttctgaa atgccttaat taggtcttca
     9421 ataatatcgg aagcttcatc atctttaggg gtaaaaatgc ttatacatga tgcttctgtg
     9481 agagaagtaa ttttagatat ttttcttgcc tttagataac tcattggttc cctcctggaa
     9541 ttattggata tatcatttca tctgtctaaa ttattcgggc tttcctcaac atatgaggga
     9601 cattcctcaa caaattcaac aaaaacagat ataaccgttc atgaggtatg tccccttact
     9661 ttgaaagcga tatgtccctt acttttactt gtctattcta atttcattag cctctttcgt
     9721 catcttgtct ccgcccaatt ttctccattc agcgataaac ttatcatatt ctgaaactga
     9781 acgtttgcca gtaataacat caaccttaaa ttccccctca agtttagaga gttcagagaa
     9841 ttcagcagca gatggcaggc tccgtacaaa ataagattta ttcataacgc ttctgcctgg
     9901 ctccgcatat aatttatctc tccaggtgga atatacaggg ttgacaaggt ttagtgcatg
     9961 gtagttattg gtaaaagtaa atacactgac taagcctgca ccatatcgct cgtctgatgt
    10021 cctatattct tcattatatg caggaaaatt attttgatcg taatcccaat gtttaccttt
    10081 tatcccatat ctgacaagaa gataatcttc gggattattg aaaccattag accattcaac
    10141 aaattttaaa aatgccgtta actttggaat gtcgtcagta aactttttag aaaatcctga
    10201 ccaatatgaa taattagtca tacctgttga tttgtcacca tatggtcctt ctaaaggata
    10261 gccatatgag taagtttctt cagggaatgt ttggttaaat aattcctggt ttgctgccgg
    10321 tgctatgcta ggatattctt ttaaccattc aaccggtctc cagtggttgt taataccttt
    10381 tgcagtaaag ccaatacgac cgctgcagaa actatgactt agagctgcat aaccgccttt
    10441 attttcaccg gttatgtatt ccgggtcaag tacaccgtct ttataccact ttgccagtaa
    10501 attcaaggct tcagtatact taggagaagt tacagggtac tgcaaagtac cattttcatc
    10561 ctcaaaaatg tccatcactc catatgctct aaaaacttga gtcatgccat ctgcagataa
    10621 agcgtatgtg tcatcttttc catttccatc cgggtcttct tttgcaaact tataaaatat
    10681 tctttctgca tcttttaatg ttctgggtat ctcttcacca aatttatcaa gccatgactg
    10741 gcgccaaatg attgccgagc catacatata ttcagcatta attcccggga aagtgaatag
    10801 ttcaccattt tcatgcttag caacagtcca gcaattatct cctaattcat cgaagtactt
    10861 tgctatatta ggagcagttg aacgcaacaa tttttctgaa atggtacccc caattccttc
    10921 attaactaag tcataaaact tagcataacc aagatttgaa tatatatcag gaatatcacc
    10981 ggatgcaaag cgtaaattca taatttcagc ggcttttgca ctttctatat actgcatatc
    11041 ccagtcaata tttgagttct cagtaaaaaa cttaaccatg tctgagttgt tgtcataggg
    11101 tgcagcagta ggggcaacaa cagttatttt gtaacgttct tctttgtttg ccgatgttgt
    11161 actagtagtt tcatcttttc cgccagttgc agttgaatta gatttgttag tagtacatgc
    11221 agcaaaaaga ctaaccaaca tgaacatcga taataatagt gccagttttc ttttcataat
    11281 aattcaatct ccttttgttt atattttata ttatatataa agggcaattt tatgaaaacc
    11341 ctatatatcc aaattaataa tttatataaa gcacaaatga ttaaagataa ttattaaaga
    11401 tcattgaaat actttatgaa gtttcagcct gaatcactca accttttaag gaacccacat
    11461 aaattccctt tacaaaatat ttctgtaaaa aaggataagt acaaagtatg ggcaaggtcg
    11521 ccacgtatat acatgcagct ttgatcatct caggttttgc atcgaaaagc atctgctcgc
    11581 taccttcaat aatttctttt gttgtacctt gaattataat acgttgaagt acaacctgaa
    11641 gtacaatctt tttagtgtca cgaatatata tcatacaatc aaaccatgag ttccaatgac
    11701 ctactgccag ccaaagcgct attgttgcaa gaataggctt ggaaacaggt aatataattt
    11761 ttataaatgt attaaaatca cccgagccat caagcctggc actttcctca agttcaccag
    11821 gtatttgaga aaagaagttg cgtattaata ccaggttata tgcaccaata ataccaggaa
    11881 tgataagaac cgctctgcta ttaattaatt tcagcttaga tatccaaaga taagttggaa
    11941 taaggcctcc tgaaaaaaac atagttacga taataaaaat tgaccaaaac actctattgg
    12001 gaaaacgttt ctttgatatt gcatatgccc ccatagatgt gaaaaatagt gacaaagtag
    12061 tacctgctac agtacggaat atagtattta aatatgctat accaatatat ttattacgta
    12121 taaccatttt ataattttcc agtgtaaatt gttgcggcca taggtaaatg ctgcccgatg
    12181 ggcttgctaa attgtcaaca gactgtacaa ataaatacca aaatggaatt attatagtta
    12241 aacaaaatag gaaaattact atattatttg taaaatcgaa gaccatactt ccgatgcctt
    12301 cattttttag atttttttgt aaaccaatct ttgccattac caaatccccc cttctttatt
    12361 aactctttta actatgagat tagtagttag tacaagagaa aaactaataa tgtttttgaa
    12421 taacccaaca gctgtagaaa aactatactg agcctgttgg attcctctgc gatatacata
    12481 agtgtcaatt atatcagaga cacggtaaac tgcttcatta taaaaattaa atatttggtc
    12541 aaatcctgca ttaattaaat tgctggagtt gagaatgaac ataacagaaa caaccggagc
    12601 aataccaggt aaagtaatat gtataatcct tttaaaacga ttagcgccat caactatagc
    12661 agcttcatac agctctggat taatattact aattgcagca agatatatta ttgtacccca
    12721 gcccacacct ttccatatac ctgttacaat aagcattggt acaaaataat ctatatcagc
    12781 aacaaaatag atgggtttac cgccataata ggaaataacc tgatttacaa agcctgtagt
    12841 aggtgaaaga aagtttataa atattcctga aagaataacc catgataaaa aatgcggtaa
    12901 ataactaact gtctgaacga tttttttata atgaacattc tttatttcat ttaacaaaag
    12961 ggaaagaata atgggtgcag gaaacccaaa aagcatccta agaaaactta tcttaatagt
    13021 attgccaaac acttgccgga aactaggtgc agtgaacaat gactgaaaat gctttagtcc
    13081 aacccaagga cttcccataa ttccatcaat aatcctataa tctttaaatg ctataactat
    13141 tccatacatt ggcgcataac agaatattcc aagacataac acaacaggca ggaacataaa
    13201 cacaagttgc cattcctttg cataattttg aaatatcagt gtacatttat ttatgtctgg
    13261 cttcgatttg tttttaagtg tcttctccaa agtgagtctc ctcctatata attacctagc
    13321 aaatgacttt atgtgagctt tgcaaggaaa atctaatttc tacatacaca ttaatctcat
    13381 caaaacggaa tgtcaattac aacattgtgc ttaaaacaac aatagcacaa taatgctatt
    13441 tgcactattg tgctattgtt gctatgatta agagagagct ttggtaaatg ccacatcaca
    13501 aatagaattg gttaaatgca caaggaacag ctcctatgca tttatagaaa caaaagaacc
    13561 atccttatgt tttacctgaa gaatttgctt aattcttcca cactttcttc tgtagttgcc
    13621 catgatgtaa caaaacgaac tgcactacgt tcttcgtcta ttttcgtttg aactgaaaat
    13681 tcaacccttt gtgccagttt ttccaataca tcattaggca ggataggaaa tatctgattt
    13741 gtgcatggcg gtgcatagaa tgaacaacca tagtctttta gtatagcagc tattttctcc
    13801 gccatcctgt tagcatgtat agccagttca aagaatagat tgtcctggaa taatacttcg
    13861 aattgcattc caataacaaa gcctttggcc atcatagctc ccctttgttt gattagataa
    13921 cggaagtcct tttttagttc ttccctgcag attaccagtg cttctcccag taatgcaccg
    13981 tttttggttc ctcctatata gaatacatcc actaatcggg caatgtctga taattgcagg
    14041 tcattggctt tacaagtcag ggcagatcct atacgtgcac catcaaggta aagaattaaa
    14101 tcattactgc ggcaaatatg gtgaatggcg gataattcag cctttgaata tatagttcca
    14161 agttccgtgg aattggatat atacaccatt ttaggctgta ccatatgttc gtcacagtga
    14221 taatccagtg ctttctggat atcagccgga gtaagcttgc catccgtaca gggcatggta
    14281 aataccttat ggcctgtagc ttcaattgct cctgtttcat gaacattgat atgccctgtg
    14341 ctggcagcaa ttacacattg gtgaggtctt aaaaatgaag aaatgaccag aagattagtc
    14401 tgtgtcccgc caggaataaa atgaatgtct gcatcttctt tttcgatttg ctttttaata
    14461 tattcatttg ctttctcgct gtgtacatcc aatccgtatc ctgtattctg ctcaaaattg
    14521 tactttatta gtcgttctaa tatgcgtggg tgtgcaccct cgctgtaatc attcataaaa
    14581 ctgtacatat tttcatctcc atcttttcat aagcatatta catctggcat actattcctg
    14641 gtatactatt tcctgcatac tattcctggc atactatttc cggcatgcaa ttcctggcat
    14701 gctactttga taatataaca ccactgttcg aaaagctctt atataaagca aaaagtctct
    14761 catactttta attgagagac tttatacttc aatatttatt aatattgttt ggggtgaata
    14821 gtgggattcg aacccacgac ctccagggcc acaacctggc gctctaacca gctgaactat
    14881 atccaccata taaccaacac taatattatt cccaaaaggg acattaccta ttgtaagata
    14941 gaattaagtt tttgtcaata gtcaaaattt aaaaaaactg gcgaatttct attaaggttt
    15001 acactatatt tcctccagtt gtcttaacca taatgtcctc tgggcatcac tgggcatgct
    15061 ccatccacct ctgggtgaaa agcttatggt gccaaccttt ggtccatcgg gcatgcatga
    15121 acgcttgaac tgctggctga aaaacctttt atagaatact tccagccatt tcttaatctc
    15181 actaccagag tatttttcac taaaagcttg ctgagcaagg aatagcactt tcctggggga
    15241 tgcaccatat cttactacat agtagatgaa aaaatcatgc aattcatatg gtccaacaat
    15301 atcttccgtt ttttgcctta tctcaccttt tggatctgga ggcaataatt ccggagttat
    15361 gggggtatca attattctat aaagaatatc tttggtactt tcgtcaacta tattatcagc
    15421 agcccaatat atcataaact taatgagggt tttaggtata ccgcagttta cgctgtacat
    15481 ggacatttga tcggcattat aagtacacca gccaagggca agttctgaca aatctcctgt
    15541 ccctataacc aggccgcctt ctttattggc aatatccata agaatttgtg tacgctctct
    15601 tgcctgcacg ttttcataag ttgtatccag ttgattaaca tcatgcccaa tatctctgaa
    15661 gtgctgcaga caagcatctt ttatatcaat ctcccttata cttgcaccaa cagcttccat
    15721 caattgaagt gaactcttat gtgttgtttt tgttgtccca aatcctggca tggttacagc
    15781 tattatgttt ttccgtggta atcccaattt gtcaaaggca ttggcggtaa ccagcagggc
    15841 aagtgttgaa tccagtcctc ctgaaatacc tattacagca tattttgaac ctgtatattc
    15901 cagccttttt accagacccc atgtttgaat ctcaaatatt tcaaggcatc ttgcggctct
    15961 tatactttca tctgccggaa taaaaggata tggatctata tgtctgtaaa gcttcttagg
    16021 cgaggatttc ccaatagcgt catctattat aatatcatct atggggaaac aaatattcct
    16081 atattgtcta aaagcagagg tagaagtcga gtaatttttg taatccacac ttagacacat
    16141 attatcttcc ataaaacatg aatttttaat tttttcagaa atcagctttt ctacatctat
    16201 gtcagcataa attatttgac tttcacttct aaacctttca ccttccccaa gaatagtccc
    16261 gttttcagat ataattgagt gtcccccgaa tacaccatcc gtagtagatt catttacacc
    16321 tgcagaagca tatatatacg cagtattgca cctcgcagac tgaagcctca caagatcctt
    16381 tttgtattcg tacagtcctg tttcctcaaa ggctgcagaa aggttgaaaa taattaatgc
    16441 tcctgctgtt gaatgaaaag aactttgcgg caaagtcatc tgaagatcgc cacccaactc
    16501 caaagcaaaa gggacggcaa gggtctgtaa attgggctgt aaattgggct caaaattggg
    16561 ctcaaaattg ggctcaaaat tgggctcagt atcgtaccgg gtgttaaact gagcactaaa
    16621 taaaagatta gtcccaaaag gcacttcctg attgcaaata tttatattgc agtttaaaat
    16681 gtttttcccc gtagaaaacc atctaccttc gttatatcct tttccatttc gtatacagca
    16741 tttggggaca actcctaaaa tacgtccttt ttgtaataca gtcccacagc tgaacaagcg
    16801 gttctgcaac cgtaatggca atccaacaat tgaaatgata tccatatcag ctgtttgaga
    16861 caaaatctcc gccagtttat aatttgcttc tcttaataaa gcctcttgaa ggaaaagatc
    16921 accacaggtg tatgctgtta ttgaaagctc agggaataaa ataaattggg caccttctga
    16981 atatgcttct cttattattc ctattatttt gtctgtgtta taactgcaat cagctacttt
    17041 aagaaccgga accgcagctg ctattcttaa aaaaccatac tttaacatag tataaaccac
    17101 cttttctaaa ccaaactgtt aaccttcaac cattcatcaa ctactcatca actactcatc
    17161 atctattcat cagcatattt cattcccaaa tgctcatatg cccttcttgt tgcaactctg
    17221 ccccgggtag ttttattaat aaatcccaat tgaagcaggt aaggttcata aacatcctca
    17281 attgtaccgg cctcttcat
//
LOCUS       DAOSDD010000081        17227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1352_length_17227_cov_4.769567, whole genome shotgun sequence.
ACCESSION   DAOSDD010000081 DAOSDD010000000
VERSION     DAOSDD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_17227_cov_4.769567"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK733_08855"
     CDS             complement(<1..191)
                     /locus_tag="PK733_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ10686.1"
                     /translation="MYKAIIVDDERIVLEGLKNTFKWEEYGFRVCGLAENGEEALNIL
                     ETTSSNLVVTDIRMPDMDGL"
     gene            complement(255..443)
                     /locus_tag="PK733_08860"
     CDS             complement(255..443)
                     /locus_tag="PK733_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10687.1"
                     /translation="MHFQSSTTEKADKRTMQDAWSEMEKFSVGLPTTAILDDGSILVV
                     YYSGVHIDLTNIYWSRIG"
     gene            complement(638..1741)
                     /locus_tag="PK733_08865"
     CDS             complement(638..1741)
                     /locus_tag="PK733_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ10688.1"
                     /translation="MIERERLIKVLKGETPDRVPWFADLGHWYRAESGEKWDLFKVSN
                     CTEGIANLHREVKAGWYIEVGSLHEEYYEDGVVREKELKGDIAVEVYRTPIGEIRMER
                     KWSPFSYSWDITKYIVENINDLKILLYAVERKKFKPKYKNWETIEKVGGDIGLGFPGI
                     AYTGLGSLMSYYMGVENTIYALYDEPELMGKYINTYNSKHLELVKLYCSSPAPHILFS
                     DNLSGDVQPSPIFKKYSYKHYKNIADLLHKADKTVSAHLDGLLNNMIGIVAECGIDVA
                     DACTPKPSGDLTPGEIRKQAGDKMILMGGISPVMWLPETSEKDFINHVKEWLELRKHN
                     SRLVQSAGDQVPPGTDIKRIKLVYDIVEEYGRY"
     gene            complement(2107..3024)
                     /gene="metA"
                     /locus_tag="PK733_08870"
     CDS             complement(2107..3024)
                     /gene="metA"
                     /locus_tag="PK733_08870"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOJ10689.1"
                     /translation="MPIKIPNHLPAAEVLSNENIFIMHEDRALHQDIRPLNIVVLNLM
                     PTKIVTETQILRLLGNSPLQVDVTLLHPKSHVSKNTPREHLIRFYNTFDEIKNRKYDG
                     MIITGAPVELLDFEDVDYWDELKGIMDWSIYNVFSTFHICWGAQAALYHHYGIPKYPL
                     DMKKFGVFSHQVRVKNQKLMRGFDDEFYAPHSRYTEVRREDIEKKSELEIMAESDKAG
                     VYIIASKNGRQVFVTGHSEYDPLTLKTEYDRDVEKGLCINVPENYFPGGDPEKPPLVK
                     WRGHASLLFSNWLNYYVYQETPYDMEKIK"
     gene            complement(3116..4051)
                     /gene="cysK"
                     /locus_tag="PK733_08875"
     CDS             complement(3116..4051)
                     /gene="cysK"
                     /locus_tag="PK733_08875"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ10690.1"
                     /translation="MSKIAKKLTDLIGNTPLLELGNYSSVNGLNARLLAKLEYFNPGG
                     SVKDRIGFAMIEDAEKKGLISKGSTIIEPTSGNTGIGLALVAASKGYRLILTMPETMS
                     SERRSLLKALGAELVLTPGTEGMKGSIKRAQELAESIPGSFIPLQFSNPSNPEIHRKT
                     TAEEIWRDTDGHVDIFVAGVGTGGTLTGTGEVLKQKNPDINIVAVEPYDSAVISGGKP
                     GPHKLQGIGAGFIPDVLNVKIVDEVFKVKNEEAFETTRNLAKTEGLLVGISSGAAVYA
                     ASEIAKRDENKGKTIVVILPDTGERYLSTDLFKEN"
     gene            complement(4076..4537)
                     /locus_tag="PK733_08880"
     CDS             complement(4076..4537)
                     /locus_tag="PK733_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ10691.1"
                     /translation="MNISTKGRYGIKAMVDIALNYSDENSDETISVKSIAERQNIPEK
                     YLEQLFSVLKKAGLVKSIRGSYGGYFLARPPKNITIGEILRALEGDLAPVDCVIEKRK
                     IGKCKRSNICITKYVWAKIRDGINHVVDEITLKQLAEGYKAETADSYMYYI"
     gene            complement(4718..6007)
                     /locus_tag="PK733_08885"
     CDS             complement(4718..6007)
                     /locus_tag="PK733_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HOJ10692.1"
                     /translation="MDERKLKFDTLQVHAGQKPDPATGSRAVPIYQTTSYVFDNSDHA
                     ARLFSLEEEGNIYTRIMNPTTDVFEKRIAALEGGIGALGVASGSAAVMYAILNITEAG
                     DGIVSASTLYGGTYNLFSSTLSKFGIKTTFVNPDDPENFRKAICENTKALYIETIGNP
                     GINLIDIEVVAQIAHDNGIPLIVDNTFGTPYLIRPMEYGADVVIHSATKFIGGHGTSI
                     GGVIVDSGKFDWTKGNKFSGLTQPDPGYHGISYTETFGSSAYIAKARVQLLRDTGACL
                     SPFNSFMLLMGLETLSLRVERHVSNTVRIVEYLKNHPLVSWVNYPGLSESKYYDLAQK
                     YFLKGVGSIFTFGLKGDIEACKRFIDSLEIFSHLANVADAKSLVIHPASTTHAQLSED
                     EQRAAGVTPDMIRVSVGIEDADDLIYDIEQAISKALS"
     gene            complement(6023..6478)
                     /locus_tag="PK733_08890"
     CDS             complement(6023..6478)
                     /locus_tag="PK733_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ10693.1"
                     /translation="MKISTKGRYGLRAMVDLAINSREGCVTLVSIAERQNVSVKYLEQ
                     VFSILKKGGLVQSVKGAQGGYVMSDNTENITVGDILRTLEGDLSIVPEQDNYCGVSGM
                     HNKQDNQGKMDRYLNKNLWRKINEEINHIVNSTTLKQLVDEYDYMVSNN"
     gene            6848..7972
                     /locus_tag="PK733_08895"
     CDS             6848..7972
                     /locus_tag="PK733_08895"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ10694.1"
                     /translation="MKKFILFTISAVLSLSLLFTACGKGKDLQNDNDGTITGTGNTQT
                     DENSKGSSNEDLKVSDFFPFTKDMHMKYKGIGNEYAEFETYVEFIRDNLMQVRNINPG
                     TSLAIVYELKDGELRKIFNKGEVYYRYDYTASAGEKEVLLKEPIKEGTSWTLKDGIKR
                     SITAIDKKIETPAGEYPALEVTTEEANSVTTDYYVKNLGHVKKVYKSKDSDFTVISEL
                     EKAESGVPYKERIIFYYPDFNKDKLAYVEKEIELFTNTDMKDVFKKEFRNIPKNSSLS
                     KVISENTRILKIVLNSPSSNDVNQSVIADFSSQLVTEMNAGTSLESMILSSITNTFGN
                     YYQTQKVGITLDGKPYESGHVLMKQDEYFTVDTKNTYEYK"
     gene            complement(7984..9522)
                     /gene="hemZ"
                     /locus_tag="PK733_08900"
     CDS             complement(7984..9522)
                     /gene="hemZ"
                     /locus_tag="PK733_08900"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HOJ10695.1"
                     /translation="MIFVKLDEKTLWYDIYNVIRLFFSNDEIRLLETGEESDIYINDI
                     MNIDSDYIYPGTVIKGGNFCVKIPDPGSFKPTNEELIHHNELSQYEELTKYKKLAQHK
                     ELTRKVYIALSEYTGRKMPWGMLTGVRPAKIPYEMMEEGMKQEEILEKMESWYMVSPQ
                     KASLAYSVAKAERKILERNMKDMVSLYIGIPFCKTRCLYCSFTSNCINKNAHLVDKYL
                     ESLKREIKIINDDILRTRGLKIQTIYIGGGTPTSINAQQLESLLRFIEQTLDLKYLEE
                     YTLEAGRPDTIDKEKLLVIKDSRVSRISINPQTMNIETLNAIGRSHTPEELEKAFYIA
                     RETGFDNINMDIIVGLPGENVAMFENTLKKINSMNPENLTVHALAVKKASKLKEELDS
                     SENLELKSLNLINDEMAKTMTDMAYQYAISMGMYPYYLYRQKNIAGNLENVGYCKPGL
                     ESIYNIQIMEEKQTIIAFGAGAVTKVFYPNENRLERAFNVKNLDDYINRVDEMTQRKK
                     KLLI"
     gene            complement(9590..10201)
                     /locus_tag="PK733_08905"
     CDS             complement(9590..10201)
                     /locus_tag="PK733_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ10696.1"
                     /translation="MVFKRLPTGMLASNCYILGDNGEGIIIDPGAKSQDILDVVEQLG
                     LKIKYIILTHAHVDHIISMDEIRYKLGVEVLIHEEDADSLSDLWRNGSNLFGIVKTFN
                     SADVLLKDGDIISVGGLELEIIHTPGHTPGGICIKVDDKVFTGDTLFRMSIGRTDLGN
                     GDSDDLMNSIREKLMKLDDKIMVYPGHGTATTIGYERKNNPFF"
     gene            complement(10302..10754)
                     /gene="dtd"
                     /locus_tag="PK733_08910"
     CDS             complement(10302..10754)
                     /gene="dtd"
                     /locus_tag="PK733_08910"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ10697.1"
                     /translation="MRAVVQRVTQANVKVKNILIGEIKKGLLVFLGIGNEDDEKDIEY
                     LAGKIVNLRVFNDIDDKMNLSLLDIKGELLVVSQFTLYGDCRKGRRPSYDMAAKPERA
                     ESLYEKFVKYCCDNYDIKVQTGKFQAEMNVELLNDGPVTLLLESKKDF"
     gene            complement(10892..13060)
                     /locus_tag="PK733_08915"
     CDS             complement(10892..13060)
                     /locus_tag="PK733_08915"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOJ10698.1"
                     /translation="MSISFNRLVERIKLYDTNCDIQTLRKAYILSRNAHKGQQRVSGE
                     PYITHPVEVAFILAELELDCTSIIAALLHDTAEDTTYTLEQLKVDFGKEVAALVDGVT
                     KLGKIPYSTKEEQQVENLRKMFLAMANDIRVIMIKLADRLHNMRTLKYLPPEKQLEKA
                     RETLEIYSPIAHRLGISKIKWELEDTCLRYIDPKGYYELVDKIATKRKEREAFITQII
                     NTLKIKTEELGIKAQIDGRPKHFYSIYRKMKEQNRTLDQIYDLFAVRLIVDSVKDCYA
                     VLGMVHELYKPIPGRFKDYIAMPKPNMYQSLHTTVIGFQGQPFEVQIRTWEMHRVAEV
                     GIAAHWKYKEGVKDKSSLDDKLTWVRQLLEWQKEMPDAREFMENFKIDLFNDEVFVFT
                     PKGDVINLPKGSTPIDFAYAIHSAIGNKMVGARVNGRIMPLEYKLNNGEIIEILTSSN
                     SGGPSRDWLKIVKSSQAKSKIKQWFKKEKREENIVRGKELIEKELKKHGLTHSQLFKP
                     EWLELVLKKYTFNNTDDLYAAIGYGAITPNRIVSRLKEEYKKTLKAEEFEQNILEGAL
                     QKSDRKKKNAPVNGVIVKGIDNCLVRLSRCCNPVPGDNIIGYITRGRGVSVHRSDCIN
                     VKNNPGDEERLIEVSWYTGSNAAYKADINLMANDRSSLLMDITNTISDAKIPLKGINA
                     RTTRDQTAIMTLTLEITDTEQLNKILRKLRKIEGVFEVTR"
     gene            complement(13236..13754)
                     /locus_tag="PK733_08920"
     CDS             complement(13236..13754)
                     /locus_tag="PK733_08920"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOJ10699.1"
                     /translation="MDLESKLRHVMDFPKPGIDFIDITPVLQDADALRQCLEEMRAKI
                     LELGEVDLIIGPESRGFIFGAPLAYMLDKGFVPIRKKGKLPYKTISVEYELEYGTDIL
                     EMHEDAVKPGDKVVIVDDLLATGGTAESNIKLVEKLGGEVVGLVYFIELSFLNGRDRL
                     KNYKVESLVKIG"
     gene            complement(13852..15624)
                     /gene="recJ"
                     /locus_tag="PK733_08925"
     CDS             complement(13852..15624)
                     /gene="recJ"
                     /locus_tag="PK733_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOJ10700.1"
                     /translation="MLKNERWLLKNPKANFCEMSKALGVSELLCKILVNRKITDLDNA
                     NSFIKSDVKRLHSPKLMKDMQKAVTIIKNKISQDRKIRIVGDFDVDGVISTYLLYIAL
                     SRCSAKVDYDIPHRIFDGYGINKNIIEKAKNEGVDTIITCDNGISAIEQIEYAKELGL
                     TVVITDHHEIPFIEDEKGSRVYRTPDADAIIDTKQIDCNYPFKHLCGAGIAFKFIQVL
                     YCEMGIPESEAYSLIEFVAIATVCDVVDLIGENRIFVKNGLEMLNNTQNIGLRALMRH
                     TGIEGKKLNVYYLGFIIGPCINASGRLDCALKGLKLLLTSNTEEADALAEELHQLNTE
                     RKDMTMKGVEEVSQIIENTNIKNDKVFVIYEPDIHESIVGIIAGKIRERYNVPTIILT
                     NGEHGVKGSGRSIEEYNMFEELLKCKDLLEKFGGHPMAAGLSLDVENVDILRERVNKL
                     TALTDDDLIPKIYIDAHIPLEYINLSLAEDLTILEPYGKGNPKPLFAEKDIRIFRGMV
                     LGANKNVLKLRLATKNNNCLDCIYFGDIKAFDNYIISRFGQNELDKMYSGISNSIKLD
                     LIFNIEINEYNGNRTAQLIMQYYR"
     gene            complement(15860..16807)
                     /locus_tag="PK733_08930"
     CDS             complement(15860..16807)
                     /locus_tag="PK733_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOJ10701.1"
                     /translation="MESLFFTINALAPTFAIVVLGYLFKRYGLFNDNFLTIANKFCFR
                     VALPCLLFKNITQYGIQGFLKGPYILFVVLSILFAVGVLMTFIPLVVHNRKKCGAIIQ
                     GIFRSNYIILGLPIVINMFGEDKIGSAAALVPFSIIVYNFMAVMVLSWFSHGKEVNPL
                     SWSLVKKIATNPLIIGSLLGIIYVLMPAELPSIILKPVFDLASLATPLALLAVGGQFQ
                     LKTVKNNRKELFLTVVGRLAIMPAIFLFIAVLLGYRGTDLAPLIVLFGGPTAVSSYVM
                     AKDMDSDSDLAAQIVIFSTLFSMVTLFGWVFLMRSLGFL"
BASE COUNT         5121 a         3642 c         2531 g         5933 t
ORIGIN      
        1 agaccatcca tatccggcat tcgaatatca gtaacaacca aattactact cgtggtttcg
       61 agtatattca atgcctcttc tccattttcg gcaagtccac ataccctaaa accatattcc
      121 tcccatttaa aagtgttttt taaaccttca agaactattc tttcatcatc tacaatgatt
      181 gccttataca tattaacacc tgccacaaaa taagtatagc atatttttta tgtaaaggat
      241 aatttgctaa tttattagcc tattcttgac cagtatatgt ttgttagatc tatgtgaact
      301 cctgaataat aaacaacaag tatgctgcca tcgtctaaaa tagcagtagt aggcaaacca
      361 accgaaaact tttccatttc actccaagca tcttgcattg tccttttgtc ggctttttct
      421 gtagtgctgc tctgaaagtg caaatccatc gtccatttgg caagatacat attccgggga
      481 atgcacaact ttgttggttt gtaccttcct gacccttgaa aactatacct ttatcaataa
      541 ttttcatata ctttactctc cttattatca atatttgcat taccaaatcc actgtatttg
      601 cattattgaa ttttctgcat ttgtggttct aaatcaatta gtatcttcca tattcttcaa
      661 caatatcata aactagtttt atccttttta tatctgttcc tggtgggact tggtcacctg
      721 ccgattggac aagtctgcta ttatgttttc ttaattcaag ccattctttt acatgattta
      781 taaaatcctt ctcactagtt tcaggcagcc acattacagg agatattcct cccataagaa
      841 tcattttatc tcctgcctgc tttcttattt ctccaggggt taaatctcct gaaggtttcg
      901 gtgtacaggc atctgccaca tcaataccgc attctgcaac aattcctatc atattattca
      961 gcaatccatc aagatgggca gaaactgttt tatctgcttt atgcaataag tctgcaatat
     1021 tcttataatg tttataactg tattttttga aaatgggcga tggttggaca tcaccggata
     1081 gattatcgct gaataatatg tgaggagccg gagaactgca gtatagtttt acaagttcaa
     1141 gatgcttact attatatgta tttatgtatt ttcccattaa ttcaggttcg tcatacaatg
     1201 cgtagatagt attttcaacc cccatataat atgacataag tgaaccaagt cctgtatatg
     1261 ctattcctgg aaagccaaga ccgatatcac ccccaacttt ttcaattgtt tcccagttct
     1321 tgtatttggg tttgaatttt tttctttcta cagcatataa aaggattttt aaatcattta
     1381 tattttcaac tatatatttg gtaatatccc atgaatagct aaaaggactc cattttcttt
     1441 ccatacggat ttcacctata ggtgttctat atacttcaac tgcaatatca ccttttagtt
     1501 ccttttctct tacaacccca tcctcataat actcttcatg aagactacca acttcaatat
     1561 accatccggc tttaacttct ctgtgaaggt ttgctattcc ttccgtacag ttcgacacct
     1621 taaataggtc ccatttctca ccactttcag ccctatacca atgtccaagg tcagcaaacc
     1681 aaggtactct atcaggtgtc tcacctttaa gtacttttat aagacgctct ctttcaatca
     1741 tttctccctc ctgttggctt aatatttaaa ctagcatact atattagaaa taataacatt
     1801 atttattaaa gaaagggtcg caaggccttc catagagaca ttcttactaa tactaatatt
     1861 ataatctaca ataatataaa tttgtttaca tttatatgta aaatttatag gtgcgtatta
     1921 gtcaataaaa ttctccaata agtactagca gttcctgaac aatcatatca ccagcttcag
     1981 ccaccaaaat acttcttata tgatcaatag agcatgttat gtacatatta taactgcatc
     2041 tattaaaaaa actatagttt taattatctg tatatctaca ttctatatat tcttcatttt
     2101 ccaaacctac tttattttct ccatatcata cggtgtctcc tgatatacat aataattcag
     2161 ccaatttgag aataacagac ttgcatgccc tctccatttt accaaaggtg gtttttcagg
     2221 gtcaccacct ggaaagtaat tttcaggcac atttatacaa agtccctttt ctacatctct
     2281 gtcatattct gtttttagag taagagggtc atattctgaa tggcctgtta caaaaacctg
     2341 cctgccgttt tttgaagcta taatgtaaac tcctgcctta tcagactcag ccatgatttc
     2401 caattcagac ttcttttcaa tatcttctcg ccttacctct gtatatcttg aatgaggagc
     2461 atagaattca tcatcaaaac ctctcataag cttttgattt ttaacacgaa cctgatgaga
     2521 gaaaacgccg aattttttca tatcaagagg atacttgggt atgccataat gatgatatag
     2581 agctgcctga gcaccccagc atatatggaa tgtagaaaat acattgtata tactccaatc
     2641 cataataccc tttagctcat cccagtaatc tacatcctca aaatcaagaa gctccaccgg
     2701 tgctcctgta ataatcattc catcatactt tctatttttt atctcatcaa aagtattata
     2761 gaaccttata agatgctcac gcggcgtatt ttttgacaca tggcttttgg gatgaagaag
     2821 ggttacatca acctgaagtg gagaattccc caatagccgc aatatttgag tttctgtaac
     2881 aatcttggta ggcataaggt tcaaaacaac tatgtttaaa ggccttatat cctgatgtaa
     2941 ggccctgtct tcatgcataa taaaaatatt ttcattgctt aacacttcag cagcaggaag
     3001 gtgatttggt attttaattg gcataaaccg ctcctttctt aaagtatggg gacacttctc
     3061 tcttgagcaa agagtatttc ttttacattg agggatgtcc ctttttgttt ctaagttaat
     3121 tttctttaaa taaatcggtt gacaaatatc tttctcctgt atcaggcaaa attacaacaa
     3181 ttgtttttcc tttgttctca tctctttttg caatttcgga agctgcataa actgccgcac
     3241 cagaagatat accaacaagc agcccttctg tttttgcaag gttccttgtt gtttcaaaag
     3301 cttcttcatt tttcactttg aaaacctcat caacaatttt tacatttagt acatccggta
     3361 taaaacctgc acctattccc tgaagtttat ggggtcccgg tttaccgcct gaaattaccg
     3421 cagaatcata tggctcaact gcaactatat taatatctgg atttttctgt ttcaggactt
     3481 ccccggtacc tgtcagtgta cctcccgtac ctactccggc aacaaatata tccacatgtc
     3541 catcagtatc cctccatatt tcttcagctg tagtttttct atggatttca ggattggatg
     3601 gattgctgaa ctgaaggggt ataaaagatc ccggtatgct ttcagccagc tcctgtgccc
     3661 tctttataga acctttcatc ccttctgtac caggagttaa aacaagttct gcgccaaggg
     3721 cttttaagag gctcctgcgc tccgagctca tggtttcagg catggtcaat ataagcctgt
     3781 atcctttgga agcagctaca agagcaagac caataccggt attaccactt gtaggctcaa
     3841 ttatagttga acccttgcta attagtcctt ttttttctgc atcctcaatc atggcaaaac
     3901 caattctatc ttttacactt ccacccgggt taaaatactc aagtttggca agtaatcttg
     3961 catttaatcc attaacactg ctgtagtttc ccagttctaa aagtggtgta ttacctataa
     4021 ggtcagttaa ctttttggct atttttgaca tattatcatc ctccggaata tttatttaaa
     4081 tataatacat ataactatca gctgtttcag ctttatatcc ttctgcaagt tgttttaatg
     4141 ttatttcgtc aactacatgg ttaataccgt ctcttatttt agcccataca tatttcgtta
     4201 tacatatatt tgaacgtttg cacttcccta tctttctttt ttcgattaca caatctactg
     4261 gtgccagatc cccctccaat gctctcagaa tttcccctat tgtaatgttc ttaggtggtc
     4321 ttgcgaggaa ataacctcca taggaacctc ttatgctctt taccagtccg gccttcttta
     4381 acactgaaaa caattgttca agatactttt caggtatatt ttgcctttcg gcaatgcttt
     4441 taacggatat tgtttcatct gaattttcat cagaatagtt taaagctata tctaccatag
     4501 ctttaatacc atatctgcct tttgtagaaa tattcaatac atcggctcct ttcatttctg
     4561 agcaagctga tggaaaccaa gctgttgagg actaaactgt agtgacagac ttctagacta
     4621 tcccgcaaag taactaagat tatctcttaa taacttatga ctgtgcttta gtaactctta
     4681 ccgcagttca accaaccggc ttaactcact tgttgaatca gctcagcgcc ttgcttatag
     4741 cctgttcaat gtcatatatt aaatcatccg catcttctat tccaacagaa acccttatca
     4801 tatcaggtgt tacgccagca gctctttgtt catcctcaga cagttgagca tgagtagtgc
     4861 tcgccggatg aataaccagt gatttggcat cagctacatt agcaagatga gagaatattt
     4921 caaggctatc aataaatctc ttgcatgctt ctatatcacc tttaagacca aatgtaaata
     4981 tagagcccac acctttgaga aagtattttt gtgccaaatc ataatatttg ctctctgaaa
     5041 ggccaggata attcacccag gaaaccagag gatgattctt aagatattct acaattctaa
     5101 ctgtatttga cacatgcctt tcaaccctca gtgaaagtgt ttcaagtccc ataaggagca
     5161 tgaatgaatt aaaagggctc aagcatgcac ccgtatccct aagcagctgt actctggctt
     5221 ttgcaatata tgctgaagaa ccaaatgtct ctgtataact tataccatga taacccggat
     5281 ctggctgggt aagtcctgag aatttatttc cttttgtcca gtcaaacttc cctgagtcaa
     5341 caatcactcc tccaatagag gttccatgcc ctcctatgaa tttcgttgcc gaatgtatta
     5401 ctacatcagc accatattcc atgggcctta ttaaatatgg cgtaccgaat gtattatcca
     5461 ctataagggg aattccatta tcatgggcta tttgggctac aacttctata tctatgagat
     5521 taatgcccgg gttgcctata gtttctatgt atagcgcttt agtgttttca catattgcct
     5581 ttctaaaatt ttcaggatca tcaggattca caaatgttgt ttttataccg aatttgctca
     5641 atgttgatga aaaaaggtta taggtacctc catataatgt gctggctgaa acaatcccat
     5701 ctcctgcttc agtaatattt aaaattgcat acattacagc tgctgacccc gatgcaacac
     5761 caagcgctcc tattccacct tcaagagctg caattctctt ttcaaacaca tcagttgtag
     5821 gattcataat ccttgtatat atgtttcctt cttcttccaa agaaaacaac cgggctgcat
     5881 ggtctgaatt gtcgaagaca taagatgttg tttgataaat aggaacagcc cttgaaccag
     5941 ttgcgggatc aggcttttga cctgcatgaa cctgtaatgt atcaaatttt aattttcttt
     6001 catccattaa ttttgccccc ttctaattat tgcttaccat ataatcatac tcatctacca
     6061 actgtttaag agtagttgaa tttactatat gattaatttc ctcatttatt tttctccaca
     6121 gatttttatt taagtatcta tccatcttac cttgattatc ctgcttattg tgcataccgc
     6181 tcacaccaca ataattatct tgttcaggaa ctattgacaa atcaccctca agagttctca
     6241 gaatatcacc aactgtaata ttctcagtgt tatctgacat cacataacct ccctgagcac
     6301 ctttaacact ttgaaccaac cctccttttt ttaatattga aaatacctgt tccaggtatt
     6361 ttaccgaaac attctgcctt tcagctatac tgactagagt tacacaccct tctctagaat
     6421 tgatagcaag gtctaccata gccctcaaac catatcttcc ttttgttgat atcttcattt
     6481 taagctccat tcctgcagca tgtgatatga ccaattccta gtattcctat atgaattgtt
     6541 actataatag tatatctgct ttctcttgtc aatattttta tgagaaatat tatgattatt
     6601 ctttgataat gccatattga aatttttatg agaaatatat tcagatttct gtgaaatata
     6661 ttaagattag aaaaagtata atatattttg gtccttttgc acttttttac cactctgatg
     6721 taacattatt atttccttct tattggttta ttattacatt tgtttgaatt gcatagaata
     6781 ttgcatagaa tatgatataa atgtgatata attgtaaact agattgtaaa ctaggaggta
     6841 gattatattg aaaaagttta ttttatttac aataagcgca gttttatcat tatctttgtt
     6901 atttactgcc tgcggaaagg gaaaagacct tcaaaacgac aatgacggca caattaccgg
     6961 tactggcaac acacaaacag atgaaaatag taaaggcagt agtaatgagg atttaaaagt
     7021 atccgatttc tttccattta caaaggatat gcatatgaaa tataaaggta taggaaacga
     7081 gtatgctgag tttgagacat atgtggaatt tataagagat aaccttatgc aggtcaggaa
     7141 tataaaccct ggtacatccc tggcaattgt atatgagcta aaagacggtg aattgagaaa
     7201 aatattcaac aaaggtgaag tctattacag atatgattat acagcttcag caggcgaaaa
     7261 ggaagttctt ttaaaagaac caattaaaga aggcacatcc tggactctta aggatggtat
     7321 taaaagaagc attactgcga tagataagaa aattgaaact ccggccggtg aatatccagc
     7381 tcttgaggtt acaaccgaag aagctaattc tgtaactaca gactattatg ttaaaaattt
     7441 aggtcatgta aaaaaagttt ataaatcaaa agatagcgac tttactgtaa tcagcgaact
     7501 ggaaaaggcg gaaagcggtg tgccatataa agaaagaata attttctact atcctgattt
     7561 taataaagat aaattggctt atgtcgagaa agaaatagag ttgtttacca atactgatat
     7621 gaaagacgta tttaaaaaag agttcaggaa tatacctaaa aatagcagtt tatcaaaagt
     7681 tatatcagag aatacaagga ttctgaagat tgtacttaat tcaccatcat ccaacgatgt
     7741 taaccaatct gtaatagcag atttttcttc tcaactggtt actgaaatga acgcgggtac
     7801 ttctttagaa tcaatgatcc taagtagtat cacaaacact ttcggcaact actatcaaac
     7861 gcaaaaagtc gggataacgc tggatggaaa gccatatgaa tcggggcatg tgctaatgaa
     7921 acaagatgaa tatttcaccg ttgatacaaa aaacacttat gaatataagt gatgaatata
     7981 catctaaatc aaaagcttct tctttctctg ggtcatttca tccacacgat taatatagtc
     8041 atcaaggttt ttaacattga aagccctttc cagtctgttt tcgttaggat agaacacctt
     8101 tgttacagcc cctgctccaa aagcaataat ggtctgcttc tcttccataa tctgaatatt
     8161 atatatactt tccagtcctg gcttgcaata acctacattt tccagatttc ctgcaatgtt
     8221 cttctgacgg tagagataat atggatacat acccattgat atagcatatt ggtacgccat
     8281 atcagtcatg gtttttgcca tttcatcatt gatcagatta aggcttttga gttcaagatt
     8341 ttcggaacta tccagttctt cctttaattt tgatgctttt ttgactgcta atgcatgtac
     8401 cgttaaattt tccggattca tactattgat ttttttaagc gtattttcaa acatggcaac
     8461 attttcacca ggtaaaccta caataatatc catattgata ttatcaaaac ctgtttccct
     8521 ggcgatataa aaagcttttt caagttcttc aggagtatga ctgcgcccta tagcattaag
     8581 cgtttcaata ttcattgtct gcggatttat gcttatcctg cttacccggc tgtcttttat
     8641 aaccagcaat ttttctttat caattgtatc cggtctcccc gcctcaagcg tatactcttc
     8701 aaggtatttc aagtccaacg tttgctcaat aaacctgagc agactttcca gctgctgtgc
     8761 attaatggat gtaggtgttc caccgccgat atatatagtt tgtattttca atccccttgt
     8821 tcttaatata tcatcattta taatttttat ctctctcttc agtgactcca aatacttatc
     8881 aaccaagtga gcatttttat taatgcaatt agaggtaaaa gagcaatata ggcatcgggt
     8941 tttacagaaa ggtataccaa tatataaact gaccatatct ttcatattgc gttccaatat
     9001 ttttctttcg gctttagcaa cactatatgc aagggaagcc ttctggggtg acaccatata
     9061 ccagctttcc attttctcaa ggatttcttc ctgcttcatt ccttcttcca tcatttcata
     9121 aggtatttta gctgggcgta cacctgttag catcccccag ggcattttcc ggcctgtata
     9181 ttcggataag gcaatataaa ccttgcgtgt taattcctta tgttgagcta atttcttata
     9241 tttggttaat tcctcatatt gggataattc attgtgatgg attaattcct cattagttgg
     9301 tttaaatgac cctggatcag gaatcttcac acaaaaattt ccacctttaa tcactgttcc
     9361 aggataaata taatcactat caatattcat tatatcattg atataaatat ctgattcttc
     9421 gccagtctcc agcaatctaa tttcatcatt gctgaaaaac aacctgataa cattgtatat
     9481 atcataccaa agggtttttt catctagctt tacaaagatc ataatcccat tcctccaaga
     9541 aaattcgttt tgctcatgac ttaagcaatg atgcgaattt tcttattcgc taaaaaaacg
     9601 gattgttttt tctttcatat ccgatcgtag ttgcagtccc atggcctgga taaaccataa
     9661 ttttatcatc cagcttcatt aacttctctc taatagaatt catcagatca tcagagtctc
     9721 catttcccag atcggttctt cctattgaca ttctaaataa tgtatcacct gtaaaaacct
     9781 tatcatcaac ttttatgcat attcctcccg gtgtgtgccc cggagtatgt ataatctcaa
     9841 gctccaatcc ccccacacta ataatatcac catctttcaa taatacatct gcggaattga
     9901 aagttttgac tataccaaaa agattggatc cgttccgcca caaatctgaa agtgaatctg
     9961 catcttcttc atgaataagt acttcgacac caagcttgta cctgatttca tccatggata
    10021 taatgtgatc cacatgtgca tgtgtaagta ttatatactt tattttcaat cctaattgct
    10081 caaccacatc caaaatgtcc tgtgactttg ctccggggtc tatgataatt ccttcaccat
    10141 tatcgcccag tatataacaa ttagatgcca gcattcctgt aggtaatctt ttaaaaacca
    10201 ttttcccgac tccttaatag ttcctctagt atatattcaa ttatttatcc cgttttttat
    10261 ctacttgtat actcctatat ttatacaagc attttccaag atcaaaaatc tttcttgctc
    10321 tccaggagca atgttaccgg gccatcgtta agtaattcta cattcatttc agcctggaat
    10381 tttccggtct gaaccttaat atcataatta tcgcaacagt atttcacaaa cttttcatag
    10441 aggctctcag ctctttcggg cttcgcagcc atatcataac ttggtcttct gccttttctg
    10501 caatcaccat ataaggtaaa ttgggatact accagaagtt ctcctttaat atccaacaaa
    10561 gacaggttca tcttatcatc aatatcatta aataccctca ggttaacaat cttaccggcc
    10621 agatattcta tatctttttc atcatcttca tttcctattc caaggaaaac aagtaaccct
    10681 ttttttattt caccaattaa tatattctta actttaacat tagcctgggt aaccctttgc
    10741 acaactgctc tcacttaata aattctcctt tcagttctta tataatactg aaaatgtact
    10801 atatatttaa tatttacctg gcaacaaaga actatcccta cttgcatttt acccgccggc
    10861 aagcaagaag catccccatt tgctgggtac actaccttgt cacctcaaat actccttcta
    10921 tttttcttag ttttcgcaat attttgttca attgctctgt atcagttatc tcaagagtga
    10981 gagtcataat tgcggtctga tcccttgttg ttctggcatt aatacccttc agagggattt
    11041 ttgcatcact gatagtatta gttatatcca ttaaaaggct cgacctgtca ttagccataa
    11101 gattaatatc tgccttatag gcagcattgg aacccgtata ccagctaacc tctatcaatc
    11161 tttcctcatc gccgggatta tttttcacat taatacaatc acttctatgg accgatactc
    11221 cccttcctct tgttatataa ccgattatat tatccccagg tacagggttg cagcatctgg
    11281 agagtcttac aaggcaatta tctattccct ttacaataac tccatttaca ggtgcatttt
    11341 ttttctttct atctgatttt tgcaaagctc cttctagaat attctgttcg aattcctctg
    11401 cctttaatgt ctttttatat tcttccttta atctggatac tatcctgttt ggcgtaatag
    11461 ctccatagcc tatggcggca tataagtcat cagtattgtt aaatgtatat ttttttagta
    11521 ccagctccag ccattctggc ttaaaaagct gactgtgggt aagtccatgc tttttcagtt
    11581 ctttttctat taattcttta ccacgtacaa tattctcttc tcttttctct tttttaaacc
    11641 attgttttat tttgcttttg gcttgcgagc tttttactat tttaagccaa tcacgacttg
    11701 gcccgccaga gtttgacgaa gtcaatattt caataatctc accattgtta agtttgtatt
    11761 caagtggcat gatccttcca tttactcttg ctcccaccat tttattgcca atagcactgt
    11821 gaattgcata agcaaaatca attggtgtag atcctttagg cagattgata acatcaccct
    11881 ttggtgtaaa aacaaatact tcgtcgttaa acagatctat cttgaagttc tccataaact
    11941 ccctggcatc cggcatttcc ttctgccatt caagcagttg tctaacccac gtaagcttat
    12001 cgtcaagact gcttttatcc ttaacaccct ccttatattt ccagtgtgct gcaatcccaa
    12061 cttcggccac cctatgcatt tcccaggttc ttatttgtac ttcaaagggc tgcccctgaa
    12121 aacctattac tgtagtatga agagattgat acatattagg tttaggcatt gcaatatagt
    12181 ctttaaacct tcccggaata ggcttgtaca actcatgtac cattcccaaa acagcataac
    12241 agtcctttac tgaatcaact atcaaacgta ctgcaaaaag gtcatagatt tgatccagag
    12301 ttctgttttg ctctttcatc ttcctgtaga tactgtaaaa atgcttgggt ctcccgtcga
    12361 tttgagcttt aatgccaagc tcttctgtct taatcttcaa agtattaatt atttgagtta
    12421 taaaggcttc tctttctttc cttttcgttg ctatcttgtc aacaagctca taataacctt
    12481 ttggatcaat atacctaagg caggtatctt ccagctccca tttaattttg gatataccaa
    12541 gcctatgggc aataggtgag taaatttcaa gggtttctct ggctttctct aactgttttt
    12601 ccggggggag atactttaga gtacgcatat tatgaagcct gtcagccagc tttatcataa
    12661 taacacggat atcattagcc atggccagaa acattttcct taggttctca acctgctgct
    12721 cttcttttgt actatacgga atcttgccca actttgtaac tccatcaaca agagcagcaa
    12781 cttccttccc gaaatcaact ttcaactgtt ccagtgtgta ggtagtatct tcagcagtat
    12841 catgtaaaag tgccgctatt atcgatgtac agtctaactc cagctcggcc aggataaaag
    12901 caacttcaac ggggtgtgta atataaggct ctccagatac cctttgctgt cctttatgag
    12961 catttcggga aagaatatac gctttcctta atgtctgtat gtcacagttt gtatcatata
    13021 gctttattct ctcaaccaag cggttaaaac taatggacat caatattccc ccatacgtat
    13081 tgggtttgct tattattatt ttactagtat tcttttctgc agtttcattt gcaatttcat
    13141 ccatatatta ttacccatta tcatgtatta ctcaatctgg tacattaact tgtgcttgct
    13201 ttggggaagc ccattcagaa atttatcaat ttcttttaac ctattttcac caatgattca
    13261 actttataat tttttaatct atccctgcca tttaagaatg acaactctat aaaataaacc
    13321 aagcctacaa cttctccacc caacttctca acaagtttaa tatttgattc tgctgtacct
    13381 ccggttgcca gcaaatcatc cactataaca accttatccc cgggttttac agcatcttca
    13441 tgcatctcca gtatatcagt accatactcc aactcatatt caacacttat agttttataa
    13501 ggtaatttgc cttttttccg tattggcaca aatcctttat ccagcatata tgctaaaggt
    13561 gctccaaaaa taaatcctct ggattccggg cctattatta agtcgacctc acctaattca
    13621 agtattttcg ccctcatttc ctccaaacac tgcctcaatg catcagcatc ctgcagaaca
    13681 ggtgtaatat caataaaatc aatacccggt ttcgggaaat ccataacatg cctgagttta
    13741 gattctaaat ccatattaat tgtcctcctg tttatttttt cagtttcata aataccataa
    13801 ataaattata tacctcaata aagaatgaag caaacaaaat aaaatttata tctatctgta
    13861 gtattgcatt atcagttgcg ctgttctatt cccattatac tcattaatct caatattaaa
    13921 tatcaagtcc aattttatgc tgttgctgat accactgtac attttatcca gctcattctg
    13981 tccaaacctg ctgataatat aattatcaaa agcttttata tcaccaaagt aaatacaatc
    14041 aagacagttg ttattctttg ttgcaagtct tagtttcagt acattcttat ttgctccgag
    14101 aaccatacct ctgaatattc ggatatcctt ctctgcaaac agaggttttg ggttcccctt
    14161 accatatggt tcaagtattg ttaagtcctc tgctaaagaa agattaatat actcaagagg
    14221 tatatgagca tcaatgtata tctttggaat caagtcatcg tctgtaagtg cagtcagctt
    14281 gttcacccgt tctctaagaa tatccacatt ttcaacatcc aatgataacc cggcagccat
    14341 gggatgccct ccgaattttt ctagtaaatc cttacatttt agcagttctt caaacatatt
    14401 atattcttct atggacctgc ctgaaccctt tacaccatgc tcaccattag ttaggattat
    14461 ggtaggtaca ttatatcgtt ctctaatttt accagctatt atgcctacga tgctttcatg
    14521 aatatccggt tcatatataa caaaaacttt atcattttta atattggtat tttcaataat
    14581 ttgggaaacc tcttccacac ctttcatagt catgtctttt ctttctgtat taagttgatg
    14641 aagttcctct gctaaagcat cagcttcttc agtattactt gtcagcagca gcttcaaacc
    14701 cttcagtgca cagtcgagtc ttcctgaagc attaatacat gggcctatta taaaccctag
    14761 ataatataca ttcaactttt tcccttcgat tcctgtatga cgcattagtg ctcgaagacc
    14821 tatattctgt gtattattca acatttcaag cccattttta acaaatatcc tgttttcacc
    14881 aatcaaatca actacatcac atacagttgc tattgctaca aactcaatca aagaatatgc
    14941 ttcagattcc ggaataccca tttcacaata taaaacctgt ataaacttaa aagcaatgcc
    15001 cgctccgcaa aggtgcttaa aaggataatt acaatctatc tgtttagtat caataatagc
    15061 atctgcatct ggggtcctat agacccggct tcccttttca tcttcgataa acggtatttc
    15121 atgatggtct gtaataacaa ctgtcaatcc cagctcttta gcatattcta tctgttcaat
    15181 agctgaaatg ccgttatcac atgtaattat agtatctacg ccttcgtttt tagctttttc
    15241 tataatattt ttattaattc catatccatc aaaaattcga tgaggtatat cataatctac
    15301 tttggcacta caacgagata gagctatgta aagcaaatat gtgcttataa ctccgtccac
    15361 atcaaaatca ccgactatac gaatttttct gtcttgagat attttatttt taatgattgt
    15421 tacagctttt tgcatatcct tcattaactt tgggctgtgt aatcttttaa catcggactt
    15481 tataaagcta tttgcgttat ccaggtctgt aatttttctg tttaccaata ttttgcacaa
    15541 taattcacta acgcccaaag ctttggacat ttcgcaaaaa ttggcttttg gatttttcaa
    15601 cagccacctt tcattcttaa gcataaaatc acctgtatcc ttttttagat atagtcattc
    15661 atttgcagca taaataccat tataaagtat ttgcattttt tatacaaatt aaattctgcg
    15721 agagggtgca tttattaaat atgatgaaat atttatcagg tgctgttgtt acggcacctg
    15781 ataaatcgta gaacaccctt acttaactgc agctataccc caccatattt gaccatattc
    15841 attgtaaaat tctatttact tacaaaaagc ccagtgacct cattaaaaat acccaaccga
    15901 aaagagttac catagaaaat aaagtggaaa agattactat ctgagctgca agatcgctgt
    15961 cactatccat atccttggcc attacatagc tggaaacagc tgttggaccg ccaaataata
    16021 caataagcgg agccaggtca gttcctctgt atcccaaaag taccgcaata aacaaaaata
    16081 ttgcgggcat gattgccaat cgtcctacaa cagttaggaa cagctctttt ctattatttt
    16141 ttactgtttt caattgaaac tgtcctccaa cagctaatag cgcaagtggt gtagccaatg
    16201 aagccaggtc aaaaacaggc ttcaatataa tggagggtaa ttctgcaggc atcagtacat
    16261 agataatacc taacaaagaa ccgataataa gtggatttgt tgcaattttt ttaacaagac
    16321 tccaggaaag tggattaacc tcttttccat gggagaacca actcaaaacc ataactgcca
    16381 taaagttata aacaataata gaaaatggta ccagtgcagc agccgatcca attttatctt
    16441 ccccaaacat gttaattaca attggaagac ctaatataat ataattgctg cggaaaatac
    16501 cctgtattat cgctccgcac ttttttctat tatggacaac taatggtata aatgtcataa
    16561 gtacacctac tgcaaataaa atagaaagaa ctacaaaaag tatatatggt cctttcagaa
    16621 agccctgaat accatattgg gtaatattct taaacaacag acaaggcaat gccacccgaa
    16681 aacagaattt gttggctatt gttaagaagt tatcattaaa caagccataa cgcttaaata
    16741 gatagcctag aacaacaatg gcaaaagtag gtgcaagtgc attaattgta aaaaaaagac
    16801 tctccaactt tctatccccc ccttagttaa acatttaatt taacttcatg caattgtttc
    16861 ttatttttct ttcagacaat tttatgagta caactttgcc cggagttgaa tttaaagctc
    16921 taagcagcgt aacgtcagct cctgttaaat atttctgcac tttaaaaata cacccataag
    16981 ctttatttta tttgtaatat tcggatatta gagtatcata atagtaaagc attgtaaaga
    17041 aacttgggtg cattttctat atttgaggga aaatatggca agaagcgatt tccatgtata
    17101 atattcactt cagcaacgga ttgtagcgtt taagtctaca gaaaaattct acccgtcaca
    17161 tttatcatcc atgtgtctcg tatgttgaca gcttatgcat acgagacaca acattaatgt
    17221 tgtgcat
//
LOCUS       DAOSDD010000082        17053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1366_length_17053_cov_5.429639, whole genome shotgun sequence.
ACCESSION   DAOSDD010000082 DAOSDD010000000
VERSION     DAOSDD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17053
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_17053_cov_5.429639"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="PK733_08935"
     CDS             complement(<1..1676)
                     /locus_tag="PK733_08935"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ10702.1"
                     /translation="MKQHLLLMHLKQFFGGIRIRFICYFIIGMILTLTLSSSLSYRTM
                     HSVIERINRRNSKAEFMQIATALNSLYENLNRQMNMLIYAPSIKSLLNYNNYSEVDII
                     YSINDFAHVVNDMFLNFPYIHSVYLFLDEGHTLCITAKNVRYFFYPRDIKKSAQVLAM
                     LEKAVRKLTLHSGLSTADFQLYSADNISIPLVSAVKQITGGTLVINLEEKEIDMLYSG
                     IASNSSCIIRLVDVNGTIFSSGNKAEIGTIYKQFHYININTPGEISYSKEEQRLLLWY
                     PALSSGNLIITNEVLLGEYWSDLSLIQRTLFLMFFLGLSITCLSFFMWMKKVFSPLNK
                     LEESMCRAGAGYYDQLLNAHGNDEISTLVWHYNEMLTNLKMLTERNKIIEQERRKSEL
                     NALRNQINPHFLFNTLNTIKWMAIIANAKPIVKSVTALGNIIAPMFKTDNPMCSLQEE
                     LQMLKYYLHIMNMRYGGGIIFETKIEEGLENVQLLRFILQPIIENAIIHGFKGNGNQG
                     TILLKAYTKGDFMYICISDNGTGMSEEELKRLNILLYNGGETKGIGIANT"
     gene            complement(1682..2905)
                     /locus_tag="PK733_08940"
     CDS             complement(1682..2905)
                     /locus_tag="PK733_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ10703.1"
                     /translation="MYRVMIVEDEMLVRIGLKSMLNWEQLGMTVIADVSNGKEALEAY
                     TTQRPDILITDLRMPVMDGLTLIRKIREQDKQLIIIILTCLDEFSLAQEALSLGVSNY
                     ILKLTMSPEEIEQILVKCRETLDKRHETSIFETQWIDRNLIKGQMLDDFLLYKSLPVD
                     VFASFVNHSRMRLSEDKLCLVIMQLPDYKSLIESSGDTRGRKFCNFLLGVLDKVFTDY
                     NRGEAYCDYKSEYILLFSFGDVEHARIPSLINEILYAVKDAITHFIGADAYFGVSSIQ
                     NGYHYLPELRIEALKALEQNVKNTLPLKIDAALSYMNAHYQDDISLETVADYAGVTPN
                     YLSYLFTHTVGESFIDTINKIRIEHAKIFLSDHSKTVYMVGEQVGFTNTTYFIRVFKK
                     HTGMTPNEYRNNLKR"
     gene            complement(3365..12736)
                     /locus_tag="PK733_08945"
     CDS             complement(3365..12736)
                     /locus_tag="PK733_08945"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOJ10704.1"
                     /translation="MKKYYNVLLSVLLSLTLLASTLLGIGSCITVNAGDEDTVLQPIE
                     NLQEPLEVDSSGEHGEVDMENNINNNDTDEPYVPKLLITELVPNPSGEGIPSDVFEFV
                     ELYNSSNQTINLKDYKLMYEVSQTDIRINDIDYDANIAPGKVAVIWIYNSDSLSKIGP
                     YENIDNNILNKFREHYNMSSDAALYTVLGSGTGTFNLQNSGKKTIMVARDDAEIGDYI
                     CIATYNEGTDTTEDTKVEDGAIIYKCPSDGSNCMVKIGNNQKPTPGEIIAKQKPINYD
                     DPDEPVIEHQTPADSVEPEPLTITAKVTDETELRTVKLYYETEKTSGMKCVKMIPEQE
                     SENSFQHTICPLDMWGSTYIKYYIEASDGLHTVTLPTNSSEPYNISINDTNKRYVPEL
                     LFTEVCPNPTGEGKETVDYFEFVELYNSSDNSINLKDYKLLYETPSSIRIINLDYNVD
                     IEPGKVAVIWIYNNDSSNKFGSFESIDGSILARFRNHYSMPDDAVLYAVFGSFEGATF
                     NLQNSGERFVMVARDDAEIGDYICVVTYNKGGGSADSGTDGSSIIYKCPTDNTNNMEK
                     MEVISPPTAGVVHDEQKPIDYDNISAAPVIVHQQPADSIDLGPLTITATVTSEAEFYS
                     VKLYYETEKTSGMKCVEMILGQDSENSFQYEIPEEDILESKYIKYYIEASDGLHTVTS
                     PTDSSEPYNISINDTNKTYVPELLITEICANSSGITDASDLFEFVEIYNSSDQKINLK
                     DYKLMYQQTADKVRSTDIDFDAYIEPGDIAVIWIYNDDALKKLAPFENINDDILSEFR
                     KHYNMPPDAALYPIIGTFDVGTSFNLQNTNERSVMIARDGAQIGDYICIATYNKGGGD
                     ADTKIDGSSIIYKCPVDNTNNMEKMEVILKPTAGEIYPEQKPINNNDPAAPVIVHEPL
                     PKSVKSGALTITAEVTDETEVRSVKLHYETEKTSGFKCIRLIPEQDNKNLYQYEIPEE
                     YILGSTYLKYYIEASDGLHTITSPTDSSEPYNISITGSDRPYVPELLITEIVANATGE
                     IDTSDVFEFVELYNTTDQTINLKSYKLMYQQTATKTRSTDIDFDAYMKPGDVAVIWLY
                     NDDSLKALGSYEDINDGILSRFRKHYNVPPDAALYSIIGTFNIGTSFNLQNTEKRSVM
                     IARDDAEIGDYICIATYNEGGGNADTAAQDGSILYKCPTDNTNCMVKMGVFPEPTPGK
                     IRAEQKPINYDDTAAPVIEYEPPVGSIEPGALTISVVVTDDDEVRTVDLYYETFNTCG
                     MKCVRMIPEQENENLFQYVIPEKDILGSAYVKYYIEASDGQNTVTSPIESSSPYITSI
                     TDNDGPSVNIMNPVDGNCFYNYYKPVISASYSDASGIDSEAIKLYLNDEDITDKAEIN
                     DNSIIYSPDVPLFEGKYIVRLEVSDNAPSKNTTIKAWTFSIVTVETQVGPVIPEILIT
                     ELLPNPIGEIINGKQDRSDVYEFLELYNASNNDINIKDYKLMYLPDKENPQDIKTLDL
                     AEDAIIYPGQTAVIWIYNMDSRKFFKTGESDTMDADMKNSAISAFRKHYNMPFSTPVF
                     LADGDSDKRPGAFHLQNTGEKTIMVARDGAEIGDYICTATYNDELDLDIKYVIESKSI
                     IYKYPTDNSNKMVKIKGGLDPTPGSITYEQRPLNYNDTVSPTIQNEPLQGEVDPAQLK
                     ITAYVEDETQVRMVKLYYETFNTPGVVSIEMNEDYTNPGTYEVGIPYKDIVGSSYIKY
                     YIEASDGINNVISLLQPGDFNTVTVKDTLGPTITKMHPIDEFYIEENYRPEIYAEYKD
                     SSSVDSSSVKLFVNETDVTSKTVKNDTYIKYIPDSDLSAGDCNVRLELCDNSPAKNKT
                     VKAWSFRIGPAVYNLYSGQIHSHSSYSDGTGTPDEAYSFARDISVVDFFGLTDHGDYV
                     SQQQWRELLDIADSYNKSDEFVTIPGFEMTWNSNTGWWGHINSYNTGWVETRVNNTSV
                     CMPNYYNNLVNTPGSVSQFNHPGFVWGDFSEYGYYSEEVDEVVNLMEVYTPDHEIEYA
                     RALDKGWHISPTNNDDTHIGDWAKYTRQTVVLAPRLTKENILESLRKNRTYSTQDREL
                     RIMYSINGYVMGSRLNDPEQLNVSIEAYHPDYNITKISVIADGRSIAASKQFNSNDVK
                     WNFELPPIYSYYYVKVEFAHNYWAVTAPIWIENSKPLMLSNLETGLDDGNNEKPVQIK
                     AEIQNDGEAVITDIKVELFRVNTIESSLLDEKSISSIGISEKREVRFNIPNIPNNIDL
                     RTVYVRVTGKAGEKVVGAVKAITIPELLITEILPNSSIDEGCNSDTYEFVEVYNNCNV
                     PVDIKDYRIEWYASNGASVKTWDLTTSKIIPPKGVLVIWIKPNDAGVKAKTLTDFNKK
                     YGTSLTNEQVVELAGVTLENTGGRTLFISRDTPVEKLSQARYNVGMNLNKDTLEDLSI
                     QYAYPNEGTVILKKLESGTAPTPGVITANQVPYVTNSLTDATLNNITINGISLKDFSP
                     TTMNYDIVLPFGTKEVPKTEAVPTVEGVECAITPASGLPGTTVILVTAKDGITTSEYK
                     INFAVSEEPEEPDEPEEPEQPEEPEQPEEPEQPEDPGEPEQKVEPEKPKPEEPKEPGK
                     PEEPEEPEPGDTEPDEAKTIIDIIDQIDEGAISKAAGKDLEKLAKDIATKISQVAELI
                     LDIEDSETVVRLTNDLIEKTRYLVAKLTEENINENYDLIEKRLNNFANTIMGRIGTLI
                     VDAETVDVDGKCEAEVIFGGTKDGELIDAEINNKLDFIVDTVKQLNDALKKAGVDDAL
                     EAILCLYAQSGSDNVVNADVKIPANLVIALEEKGINRMKVETDIATFIVPTDAIALEN
                     ASSITLNSEILNVSELSDEIKSLVGDAPVYSFCITADDERVKNFNRPIMVSVPYIPKP
                     GEDMEKMTVFYINENGELENIIGVYDEQTGRVFFKTEHFSKYAIKLNDVKFSDVTSQF
                     WAARYIEVMASKEIIKGIGGNMFAPDKNVTRAEFATMIVRVFKLFDDKAENIFSDVNT
                     SDWFYREVISAASFGIVQGRPGGIFAPNDKITREEMAAMLSNVLKGIMGKKVPENKAA
                     YLDTFKDSSDIASYAKDAVAIGTRYKFFTGKPDGTFAPKDTANRAEASKLIYMLFDIL
                     FSLKS"
     gene            complement(13021..13179)
                     /locus_tag="PK733_08950"
     CDS             complement(13021..13179)
                     /locus_tag="PK733_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ10705.1"
                     /translation="MYDSGWHIHSEAFYDASIPVEELIASAKDTGLKQFGITDHANIN
                     IPSFYMYK"
     gene            complement(13301..15055)
                     /locus_tag="PK733_08955"
     CDS             complement(13301..15055)
                     /locus_tag="PK733_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10706.1"
                     /translation="MLKKCIKNLSLVISILLVLSVISGCITKDKEDTKDKGASKLSDT
                     ISSEGDVRPMKDNLYLQGIPIVKNRETFDLMITYGSGHVDTNDLPLYKEREERTNVKL
                     NYIYVMSTTLNERKMTMFATNDFPDAMVGIIKNDDIVRYSPTGIFLPVDEYVDKYMPN
                     LKKLMKEYPDVKGMSKMPDGKMYGFPRLNFWSVWPGGDTYINTTWIINQEWLDTLKLK
                     TPETTEEVKEVLMAFKTKDPNGNGKPDEIPYSFCYSDGRSFRESMFGPFGLIGPGKKE
                     NVENGKVFFVATDPRYKDVVKYVRDLYTNGLIDKEAFSHTREQFRSKGVQADPPIYGM
                     TNGWMGDDEVGPRIRTPGNSDAPYVPMPPVAGPNGTRLWGNQIEGIVPHIFVVSSKCK
                     SPETLARYVDELYSEDESVQECWGAFGEWVEKRDDGTYARRITPEGRNTTEWMMESSS
                     RMMPYALTDALVERMRIQSYDMKIEDEAERKMEKNIIYLGKDAETKYIYSVVYAPYAK
                     KEVYPGGVIRTPEEIDEIARLLPQIQNLIEKKEVEWMTGVADIDAEYDDFIKKLKEYK
                     IDRITELYQAAYDRYMGK"
     gene            complement(15156..16091)
                     /locus_tag="PK733_08960"
     CDS             complement(15156..16091)
                     /locus_tag="PK733_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10707.1"
                     /translation="MKNEKRKSRIRNIAENGISGDDVFDFFNIVLLTIFAIIVFYPMY
                     FVVIASISSPSAVASGQVVLLPKGNNFLGFQRVLRDERLKTGYLNSTIYTVVGVIVNL
                     VMTLPAAYVLSRDDFKLKKPLTIFIIFTMFFGGGMIPNFIVVKQLGLLDKIWALVLPG
                     AISTSKLILARTFFQTTIPDELFDSALIDGCGNFKFFINIVIPISGAIIGILALQYGI
                     EHWNAYGSALLYIYNPKKYPLQLILRDILLRSQITAKQMESEESDALLSVLMQQISES
                     IKYVIVIAACLPIVVVLPLIKKYFAKGIMIGSIKG"
     gene            complement(16137..>17053)
                     /locus_tag="PK733_08965"
     CDS             complement(16137..>17053)
                     /locus_tag="PK733_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10708.1"
                     /translation="MKTKGRIRKELIKNYDLYLMALPMILYYIIFHYWPLYGIQMAFR
                     DYNIVLGFLKSPFVGLKHFRNFFSLPIAFMYIKNTLRINLTYLMLYTPVPVLLALMLN
                     SLNKEKTKNRIQAVTYVPQFISTVIAVAMIRIFLNPYTGIVNIILTKLGIEVIDFLNK
                     PELYAYVYVIPGILQYAGWSSLIYTGALTGINPELYEAARIDGASKFRQIINIELPII
                     AKVFVIQLILKMGGLMSLGWEKSYLLQTPFNIEVSEIISTYTYKVGIIGMQYSYSAAI
                     GLFNSVVNFILLLIANFVSRQVSESSLW"
BASE COUNT         5009 a         3379 c         2596 g         6069 t
ORIGIN      
        1 cttgtattag caattcctat acctttagtt tcccctccgt tgtatagcaa gatattaagg
       61 cgctttaatt cttcttcaga cataccagta ccattatctg atatacatat atacataaaa
      121 tcaccttttg tataagcttt taatagtata gttccttgat ttccgtttcc tttaaatcca
      181 tgaataatag cattctctat tatcggttgg agaataaaac gcagcaattg tacattttct
      241 agcccttctt caattttagt ctcaaatata atacctccac cgtagcgcat attcataatg
      301 tgcagataat acttaagcat ttgtagttct tcttgcaagc tgcacatagg attgtccgtt
      361 ttaaacatgg gagcaataat gtttccaagg gcagtaactg atttaacaat aggttttgca
      421 ttagctataa ttgccatcca ttttattgtg tttaatgtgt taaataagaa gtgcggatta
      481 atctggttac gtaaggcatt taattcgctt tttcgtcgtt cctgctctat aattttattt
      541 cgttcggtta acatcttcaa gttcgtaagc atttcattat aatgccacac caaagttgat
      601 atctcgtcgt ttccatgagc attcaacaac tgatcataat aaccggcacc tgcacggcac
      661 atgctttctt ctaacttatt cagcggtgaa aacacctttt tcatccacat aaagaaggat
      721 agacaggtaa tacttagacc taaaaagaac ataaggaaca gtgtcctttg aatcaatgat
      781 aaatcactcc agtattcacc aagtagtact tcatttgtaa taataagatt gccagatgac
      841 aaagcgggat accaaagaag cagacgctgc tcctctttac tgtatgatat ttcacctggt
      901 gtatttatat ttatatagtg aaactgttta tagatagttc cgatttctgc cttattgcca
      961 gaagagaaaa tggttccatt tacatctaca agacgtatga tgcaactgga attggatgct
     1021 attccactat aaagcatatc aatttccttt tcttccagat taattacaag tgtaccacca
     1081 gttatttgtt ttactgcact aacaagtggt attgatatat tatctgctga gtaaagttga
     1141 aaatccgcag tggataaacc actgtgaagt gtaagctttc ttacagcttt ttcaagcatc
     1201 gcaagtacct gagctgattt tttgatatcc cggggataga aaaaataacg tacattttta
     1261 gcagtaatac aaagagtatg cccttcatct aaaaacagat aaacagaatg tatgtatgga
     1321 aaattcaaga acatgtcatt aaccacatga gcaaaatcgt taattgaata aattatatcc
     1381 acctcggaat aattattgta attgagcagt gatttgattg aaggtgcata aataagcata
     1441 ttcatctgtc tgttaaggtt ttcatataaa ctatttaaag ccgtagcaat ctgcataaat
     1501 tcagctttag aattgcggcg gttgatacgc tcgattacag aatgcattgt gcggtaactt
     1561 aatgaagatg ataaggtaag tgttaatatc attcctatta taaaataaca tataaatcgt
     1621 atacgtatgc cgccaaaaaa ttgtttaaga tgcatcaata acaaatgttg cttcacgcag
     1681 ttcacctctt caaattgttc cggtattcat taggagtcat acctgtatgt tttttaaata
     1741 cacgtataaa atatgtggta ttagtaaatc ctacttgttc accaaccatg taaacagttt
     1801 tactatggtc cgaaagaaat attttagcat gttcaatacg gattttgtta atagtatcta
     1861 taaacgattc accaacagta tgtgtgaaaa gataacttaa atagtttggt gttactcctg
     1921 cataatccgc cactgtttca agggaaatat catcttgata atgtgcattc atataagata
     1981 atgctgcgtc tatcttgagg ggcaaggtgt ttttcacgtt ctgctcaagc gctttcaatg
     2041 cctcgatacg aagctcagga aggtagtgat agccattttg aatagaacta acaccaaagt
     2101 atgcatctgc acctataaaa tgggtaattg catccttaac agcatacagg atttcattaa
     2161 tcagggatgg gatccgtgca tgttctacat ctccaaaact aaagagtaag atatattctg
     2221 acttgtaatc acaataggcc tccccacgat tataatctgt aaatactttg tccagaacac
     2281 ccaataaaaa gttgcagaat ttgcgtccac gggtatctcc gcttgattcg ataagggact
     2341 tatagtcagg taactgcatt ataacgagac atagcttatc ttcggacaga cgcatgcggg
     2401 aatgattaac aaaggaagca aaaacatcaa caggcaagct tttatataat aagaagtcat
     2461 ctagcatttg tcccttaatc aaattccggt cgatccattg agtttcaaag atactggttt
     2521 catgtcgttt atccagtgtt tcacgacatt ttacaagaat ctgttctatc tcttccggac
     2581 tcatagtcag ttttagtata tagtttgata caccaagaga aagcgcttct tgagcaagtg
     2641 agaattcatc aaggcatgtt aatatgataa ttattagttg cttatcctgt tcccgaattt
     2701 tgcgaatcaa tgtcaaaccg tccatcaccg gcattcgcag gtctgtaatt aagatgtccg
     2761 ggcgttgagt ggtataggct tccaatgctt ccttaccatt ggaaacatcg gcaattactg
     2821 tcatccctag ctgctcccaa ttgagcatac tctttagtcc tattcgaaca agcatttcgt
     2881 cctctacaat cattacacgg tacattataa atccaccccg tactaataca agataatcct
     2941 ttgatatttt atttcatttt cattataaca tattttatat catattttca cgtcctttgc
     3001 tacttccttt ttcttgcatt ttcctactct tatcacgaac cctttcagag gaagacagct
     3061 attttgatcc tttcttcctg gaaattaatt gttattaacc tatcttaatt cataaatgtt
     3121 actggtcaat ttcagtcctc ctcgttttgt ttatttgaga ttgctctgtt cacaggtgta
     3181 tgtttcctca taaaagaagc atcctctatg actcaccctg tcaaatggct tatagtatat
     3241 cgcagctaag ctgcaagctt tcgaagtgtg aaaaacgctg cgataaatta aggtgctgta
     3301 tttcctcaaa aagccatgta gcggttgcac tacatggctt tgaataaaat gtttgttttt
     3361 attattaact ttttaatgaa aatagtatat cgaaaagcat ataaattaac ttggatgctt
     3421 cagctctgtt tgcagtatcc ttaggtgcaa aggtaccatc aggttttcct gtaaagaatt
     3481 tatacctggt tcctattgct acagcatctt ttgcatagct tgcaatatca ctgctatctt
     3541 taaatgtatc caaataagcg gctttgtttt ctggaacctt tttccccata attcctttaa
     3601 gtacattgga aagcattgca gccatttctt ctcttgttat tttatcatta ggtgcaaata
     3661 ttcctccggg ccttccctgt acaataccaa agcttgcagc tgatattact tcacgataga
     3721 accagtcgct tgtattgaca tcgctgaaga tgttctctgc tttatcatca aacagcttaa
     3781 ataccctgac tatcattgtt gcaaattcag ctctggtaac atttttatca ggtgcaaaca
     3841 tgtttccacc tatacctttg ataatttcct tagatgccat aacttcaatg tatcttgcag
     3901 cccaaaattg gcttgttaca tcactaaact taacatcatt aagtttaatt gcatatttgc
     3961 taaaatgctc agttttaaag aatactctac cagtttgctc atcatataca ccaatgatat
     4021 tctcaagctc accattttcg ttaatataga atacagtcat tttttccatg tcttctccgg
     4081 gctttggtat ataaggtact gataccataa taggcctgtt gaagtttttc actctctcat
     4141 cgtctgctgt aatgcaaaaa ctatacacag gagcatctcc cacaagactc tttatttcat
     4201 ctgatagttc actaacattc agaatctcag aattaagagt tattgagctg gcattttcaa
     4261 gtgcaatagc atctgttgga actataaatg ttgcaatgtc agtttcaacc ttcattctgt
     4321 tgataccctt ttcttcaaga gcaataacca gatttgcagg tatcttcaca tcagcattaa
     4381 ctacgttatc agagccgctc tgtgcataga ggcaaagaat agcttctaga gcatcatcaa
     4441 ctcctgcttt tttgagagca tcgttcagct gtttaaccgt gtccactata aagtcaagtt
     4501 tattatttat ttcagcgtct ataagttccc catctttagt tccgccaaat attacttcgg
     4561 cttcacattt tccatctaca tctacagttt cagcatcgac gattaaagtt cctatcctac
     4621 ccattatagt atttgcaaag ttatttaacc gcttctcaat taaatcataa ttttcattta
     4681 tattttcttc tgtaagtttt gcaaccaaat atctagtctt ttctatgaga tcatttgtaa
     4741 gtctaactac agtttcagag tcttcaatat ctaaaataag ttcagcgacc tggcttatct
     4801 ttgttgctat atctttcgca agcttttcta aatctttccc agctgctttt gatatagcac
     4861 cttcgtctat ttggtcaata atgtcaataa ttgtttttgc ttcatccggt tcagtatcac
     4921 ctggttccgg ttcttcaggc tcttctggct ttcctggctc ctttggctct tcaggcttag
     4981 gtttttctgg ttctactttt tgttcaggct ctcccggatc ttccggttgt tctggttctt
     5041 ccggttgttc tggttcttct ggttgttctg gttcttctgg ttcatcaggt tcttcaggct
     5101 cttcagaaac tgcaaagtta atcttatact cgctggttgt tattccatcc tttgctgtta
     5161 ccaaaataac agttgttcca ggcagtcctg atgctggagt tatagcacat tcaacacctt
     5221 caactgtagg tactgcttct gttttaggta cctctttagt gccaaatggt aatacaatgt
     5281 catagttcat tgttgtcgga ctaaagtctt taagggatat accattaata gtaatattat
     5341 tcagcgtcgc atccgtcaaa gagtttgtaa catatggaac ttggttcgca gtaatcactc
     5401 caggtgtcgg agcagtacca gattcaagtt ttttcaatat aactgttcct tcattgggat
     5461 aagcatactg tatgctcaaa tcctcaagtg tatccttatt aagattcatt cctacattat
     5521 atctggcttg cgatagtttt tcgactggtg tgtctcttga aataaatagt gttcttccgc
     5581 cagtattttc aagggtcact cctgccaatt ccactacctg ctcattagtc aggcttgttc
     5641 catatttttt gttgaagtct gtcaatgttt ttgccttcac tccagcatca ttaggtttta
     5701 tccatattac taaaacaccc tttggaggta tgattttgct tgttgtcagg tcccaagttt
     5761 taactgatgc accattgctt gcataccatt caatcctgta atctttaata tcaacaggca
     5821 cattacagtt gttataaact tcaacaaatt cataagtatc actgttacaa ccttcatcta
     5881 ttgaagaatt aggcaatatt tctgtaataa gcaattccgg tattgtgatt gcttttacgg
     5941 ctccaactac tttttcacct gcttttcctg tcaccctgac ataaactgta cgtaaatcta
     6001 tgttatttgg aatatttgga atattgaatc ttacttcacg cttttcactt atccctatag
     6061 agcttattga tttttcatct aataaactac tttctatagt attaaccctg aataactcaa
     6121 ccttaatatc tgttattact gcctctccat cgttttgtat ttccgctttt atctgtaccg
     6181 gcttttcatt attcccatca tctaacccgg tctcaagatt tgacagcatt agcggcttac
     6241 tattttcaat ccaaattggt gcagtcacag cccaataatt atgagcaaat tcaactttaa
     6301 catagtaata actatatata ggaggaagct caaaattcca cttaacatca tttgaattaa
     6361 actgctttga tgctgctatg gaacgtccat ctgctataac actaatttta gtaatattat
     6421 aatcaggatg gtatgcttct atgcttacat taagttgttc agggtcatta agccgtgaac
     6481 ccatgacata tccgttaatg ctgtacatta ttcttaattc tctatcttgt gtggagtaag
     6541 tcctattctt acgcaatgac tccagaatat tttctttcgt cagtcttgga gcgagaacaa
     6601 cagtttgtct tgtgtacttt gcccaatctc caatatgggt gtcatcattg tttgtaggac
     6661 taatatgcca tcctttatca agagctctag catattctat ttcatgatct ggtgtatata
     6721 cctccataag atttacaacc tcatctacct cttcactata gtatccatat tcactaaaat
     6781 ctccccatac aaagccgggg tggttaaact gtgaaactga acctggtgta ttcactaaat
     6841 tgttataata atttggcata catacagatg tattgtttac ccttgtttca acccatcctg
     6901 tattatatga atttatatgt ccccaccatc cagtgttgga attccatgtc atctcaaagc
     6961 caggaattgt tacaaattca tccgatttat tgtaactatc tgcaatatcg aggagttccc
     7021 tccattgttg ctgagaaaca taatctccat gatcagtcaa cccgaagaaa tctactactg
     7081 aaatgtcacg agcaaaagaa taagcttcat ctggagtacc tgtaccatca gaatatgaag
     7141 aatggctgtg aatttgaccg ctatataagt tataaacagc cggaccgatt ctgaatgacc
     7201 atgcttttac tgttttgttt ttagctggag aattatcaca caactctaat cttacattgc
     7261 agtctcctgc actcaagtct gaatcaggta tatatttaat atatgtgtca tttttaactg
     7321 tctttgaagt aacgtctgtt tcatttacaa acaacttaac agaggaagaa tcaacagatg
     7381 atgaatcctt atattctgca tatatttcag gtctataatt ttcttctata tagaattcat
     7441 caattgggtg catcttcgtt attgtagggc ctaaagtatc ttttacagtt acagtattaa
     7501 aatcccctgg ttgcaatagt gaaatcacgt tattgattcc atcacttgcc tcaatataat
     7561 attttatata agaagatcca acaatgtctt tatacggtat accaacctca taggtacctg
     7621 ggtttgtata gtcttcattc atttctatac tcacaactcc aggagtatta aatgtttcat
     7681 agtaaagttt taccattctt acctgtgttt catcttctac ataggctgtt attttcaatt
     7741 gtgcaggatc tacttctcct tgcaatggtt cattttgtat tgtaggacta actgtatcat
     7801 tataattcaa aggtctttgc tcatatgtta tgcttccagg tgtaggatct aagccacctt
     7861 taatctttac cattttgttt gaattatcag tcgggtattt atatattata cttttactct
     7921 ctataacata tttaatatct aaatccagtt catcgttata cgtagccgta catatataat
     7981 caccaatttc tgcgccatct ctggctacca taattgtctt ttcaccagtg ttttgcaaat
     8041 gaaaagctcc aggcctttta tcactatctc catccgccaa aaatacaggt gtactgaaag
     8101 gcatgttata atgttttctg aatgctgaaa tagcactatt cttcatatcc gcatccatag
     8161 tatcactttc gccggttttg aaaaactttc ttgaatccat gttataaatc caaataactg
     8221 cagtttgacc gggatatatt attgcgtctt cagctaaatc taatgttttt atatcttgcg
     8281 gattttcctt atccggtaaa tacatcagtt tataatcttt aatgttaata tcattattgc
     8341 ttgcattata tagctccagg aattcgtaaa catcacttct atcctgcttt ccattaatga
     8401 tttcaccaat cggattggga aggagctcag taatcaatat ctcgggaata acaggcccta
     8461 cctgcgtctc tactgtaact attgaaaatg tccatgcctt aatagttgta ttctttgaag
     8521 gtgcattgtc gcttacttca agcctgacaa tatatttacc ttcaaaaaga ggaacatcag
     8581 gtgaatatat gattgaatta tcgtttattt ctgctttgtc tgttatgtcc tcatcattca
     8641 gatacaattt gattgcttct gaatcaattc ccgaagcatc tgaataactt gcacttataa
     8701 caggtttata gtaattataa aaacagtttc catctaccgg attcataatg tttactgatg
     8761 gtccatcatt atccgtaata gaagtaatat atggactact gctctctatt ggtgacgtaa
     8821 ctgtattctg accatcactt gcttcaatgt aatatttaac atatgctgac cctaaaatat
     8881 ccttttcagg aattacatat tggaataagt tttcattctc ttgttccggt atcatcctca
     8941 cgcatttcat accacatgtg ttaaaagttt cataataaag atcaacagta cgaacttcgt
     9001 cgtcatctgt tactacaaca gatatcgtta atgctccggg ctctatactg cctaccggtg
     9061 gctcatactc tatcacaggt gcagcagtgt catcgtaatt aatgggtttt tgttctgctc
     9121 ttatcttccc aggagtaggt tctggaaaga cacccatttt taccatacaa ttagtgttgt
     9181 ctgtaggaca cttgtataaa attgaaccgt cctgagctgc agtatctgca tttcctccac
     9241 cttcattata tgtcgctata cagatgtaat caccgatttc tgcatcatct ctggctatca
     9301 tgacagatct tttttcggta ttttgaaggt tgaatgatgt ccctatattg aaagtaccga
     9361 taatcgaata caaagcagca tcaggcggca cattataatg ttttctgaat cttgaaagta
     9421 taccgtcgtt tatatcttca taagaaccaa gtgcttttaa agaatcatca ttatataacc
     9481 atattactgc aacatctcca ggtttcatat aggcatcaaa atctatatca gtgcttcttg
     9541 ttttcgttgc tgtttgctga tacattaatt tataactttt caggtttatt gtttggtcag
     9601 tagtattata aagctcaaca aactcaaaaa cgtcacttgt gtctatttct ccagtggcat
     9661 tagcaactat ctcagtaatc aacagttccg ggacataagg tctatcacta cccgttatag
     9721 aaatattgta tggttcactg ctatctgttg gtgatgtaat tgtatgcagt ccgtcacttg
     9781 cttcgatata atatttaaga tatgttgacc ccaaaatata ttcttcagga atttcatatt
     9841 ggtataagtt tttattatct tgttccggta tcagtcttat acatttaaat cctgaagttt
     9901 tttcagtttc atagtgtaat ttgacacttc gaacttctgt ttcatctgtt acttcagcag
     9961 ttattgtcag agctcccgat tttacacttt tggggagcgg ttcatgaact atcacaggtg
    10021 cagcagggtc attgttatta attggttttt gttccggata tatttccccc gcagtaggtt
    10081 tcagaataac ttccattttt tccatgttat tagtgttgtc tacaggacat ttgtatataa
    10141 ttgaactgcc atcaatttta gtatctgcat ctccgccacc tttattatat gtcgctatac
    10201 aaatgtaatc accaatttgg gcaccatctc gagctatcat gacagatctt tcattggtat
    10261 tctgaaggtt gaatgatgtc cctacatcga aagtaccgat aatcggatac aaagcagcat
    10321 caggcggcat attataatgt tttctgaatt cggaaagtat atcgtcattt atgttttcaa
    10381 aaggagcaag tttttttaaa gcatcatcat tgtatatcca tattactgca atatctccag
    10441 gttctatata agcatcaaaa tctatatcag tgcttcttac tttatctgca gtttgttgat
    10501 acattaattt atagtcttta aggttaattt tttggtcact actattatag atttcaacaa
    10561 actcaaataa atcgcttgcg tctgttattc cggaagagtt agcacatatc tcagtaatca
    10621 acagttccgg gacataagtc ttattagtat cgtttataga aatattgtac ggttcactgc
    10681 tatctgttgg tgacgtaact gtatgcagtc catcacttgc ttcaatataa tatttaatat
    10741 attttgactc caaaatatct tcctccggaa tttcatattg gaatgagttt tcactatctt
    10801 gtcccaatat catctccaca catttcatac ctgaagtttt ttcagtttca tagtatagtt
    10861 taacactata gaactctgct tcagatgtta cggtagcagt tattgtcaga ggtccgagat
    10921 ctatgctgtc tgccggttgc tgatgaacta tcacaggtgc agcagaaata ttgtcgtaat
    10981 caattggttt ttgttcatcg tgcactacac ccgcagtagg cggtgaaata acctccattt
    11041 tttccatgtt attagtatta tctgtcggac atttgtatat gattgaactg ccatcagttc
    11101 cgctatctgc acttccacca cccttattat atgtcactac gcaaatgtaa tcaccaattt
    11161 ctgcgtcatc tctggctacc ataacaaacc tttcaccgga attttgaagg ttgaatgtag
    11221 cgccttcgaa gctaccgaaa actgcatata aaacagcgtc atctggcata ctataatgat
    11281 ttctgaatcg tgcaagtata ctgccatcta tgctttcaaa agaaccgaat ttattgctag
    11341 aatcattatt ataaatccat attactgcaa ctttaccagg ttctatatca acattataat
    11401 ctaaattaat aattcttatt gatgatggag tctcatacaa taatttataa tcttttaagt
    11461 taatactatt atcactactg ttatatagct caacaaattc aaaataatct actgtttctt
    11521 ttccttctcc ggtagggtta ggacacacct cagtaaacag cagttcaggg acataacgtt
    11581 tattggtatc atttatagaa atattgtacg gttcactgct atttgttggt aatgtaactg
    11641 tatgcagtcc atcacttgct tcgatataat atttaatata tgttgacccc cacatatcta
    11701 aaggacaaat tgtatgttgg aatgagtttt cactctcttg ttccggtatc atctttacac
    11761 atttcatacc tgaggttttt tcagtttcat agtataattt aacagtacga agttctgttt
    11821 catctgttac tttagcagtt atcgtcagag gttcaggttc aacactgtct gctggtgtct
    11881 gatgctctat tacaggctca tcagggtcat cgtaattaat gggtttttgt tttgcgatta
    11941 tttcccccgg agtaggtttc tggttgttac ctatctttac catgcaatta gatccatctg
    12001 aaggacactt gtaaataatt gcaccatcct caacttttgt atcttccgtg gtgtctgtac
    12061 cttcattata tgtcgctata caaatgtaat caccaatttc tgcgtcatct ctggctacca
    12121 tgatagtctt tttgccagaa ttttgaaggt tgaaggtacc tgtgccgcta cctaaaactg
    12181 tatacaaagc agcatcagac gacatattat aatgttctct gaatttatta agtatattgt
    12241 tgtctatatt ttcataagga ccaattttgc ttaaagaatc actattgtaa atccatatta
    12301 ctgcaacttt tccaggtgct atatttgcat cataatctat atcattaatc cgtatatctg
    12361 tttgacttac ttcatacatt aatttataat cttttaggtt aattgtttga ttactactgt
    12421 tatatagctc aacaaattca aaaacatcac ttggtattcc ctctccggaa ggattaggaa
    12481 ccagctcagt aattagcagt ttcgggacat aaggttcatc agtatcgttg ttgtttatat
    12541 tattttccat atcaacttcc ccatgctcgc cactactatc tacttccagg ggttcctgta
    12601 aattctcaat tggttgcaac acagtatctt catcacctgc atttaccgtt atacaggagc
    12661 caatccccaa taatgtactt gctagtaagg ttaatgatag taatactgat agtagtacat
    12721 tataatattt tttcatattt attaactcct ttcttactcc ttgtaatctc aacttgttgt
    12781 tttcattttc acgatgatca agataattcg gatcatgcga atcagaacca aatattatct
    12841 tgataccata tcctcaagag ttagcataaa ttcaggttca cagggcttat catttaacag
    12901 aaaagtgtag ccatcctttg tgccatattt agcacaataa tcattcaatg attttgaaat
    12961 aggtgtaagc tctacgctat aataaaaacc ctcacactga tatttctcat ataagagctt
    13021 ttatttatac atataaaagg aagggatatt aatattagca tgatctgtta taccaaattg
    13081 ctttagcccg gtatcctttg ctgaagcgat tagctcttcg acagggatgc ttgcatcata
    13141 aaatgcttca gaatggatgt gccagccgga atcatacata aaaaataccg cctttctttt
    13201 taatgtaaat atttcccata aggtaataat tatcacccta tgggaaacta taagcacatt
    13261 taaaacaagc acaaaacaaa tatccaattc tttaattaac ttatttcccc atatatctat
    13321 cataagcagc ttgatatagt tcagtaattc tatcaatctt atattctttc aattttttaa
    13381 taaagtcatc atattctgca tctatatcag caacccctgt catccactca acttctttct
    13441 tttctattaa attctgtatc tgtgggagca agcgggcaat ttcatctatc tcttcaggag
    13501 ttcttattac tcctcccgga tagacttctt ttttagcata aggagcataa accaccgaat
    13561 aaatatattt agtttcggca tctttcccca agtaaatgat atttttttcc attttccttt
    13621 ctgcttcgtc ttctattttc atgtcgtaag attgtatacg cattctttct acaagtgcat
    13681 ctgtcaaagc atacggcatc attcttgaag aagattccat catccactcc gtagtattac
    13741 gtccttcagg tgtaatcctg cgtgcataag ttccatcgtc tcttttttca acccattcac
    13801 caaatgcacc ccagcattcc tgcacactct catcttcaga gtacaattca tcaacatacc
    13861 ttgcaagtgt ttcaggtgat ttacatttgc tactcactac aaatatatgg ggtactatac
    13921 cttctatttg attaccccat aatcttgtac cattcggtcc cgctacagga ggcataggta
    13981 catatggtgc atcagaatta cccggagttc ttattcttgg acctacttcg tcatcaccca
    14041 tccatccatt tgtcattcca tatataggcg gatcagcttg tactccttta cttctgaatt
    14101 gttccctagt gtgtgaaaag gcttctttat caatcagtcc atttgtataa agatctctta
    14161 catatttaac aacatcctta tatcttggat ctgtagctac aaaaaagact ttcccatttt
    14221 caacattttc ctttttaccc ggacctatta acccaaaagg tccaaacatg gattctctaa
    14281 aagaacgacc atcgctataa cagaacgaat atggaatttc atccggtttt ccattaccat
    14341 tcgggtcttt tgtcttgaaa gccatgagaa cttcttttac ttcttcagta gtttcaggtg
    14401 tttttaattt cagtgtatca agccattcct gattaataat ccatgtagta tttatgtatg
    14461 tatctcctcc aggccatact gaccagaaat taagccttgg aaaaccatac atttttccat
    14521 ccggcatttt tgacattcct ttgacatccg gatattcttt cattagcttt ttcaaattgg
    14581 gcatatattt atcgacatac tcatcaacag gaagaaaaat cccagtaggt gaatatctta
    14641 caatgtcatc attttttatt atgcccacca tagcatcagg aaaatcattt gttgcaaaca
    14701 ttgtcatttt tctttcattc aatgtagttg acataacata gatgtaattc aatttcacat
    14761 ttgttctctc ttctctttct ttgtaaagag gtaaatcatt agtgtctaca tgtccagatc
    14821 cataagtaat catcaagtca aaggtttctc tattctttac aattggaatg ccttgaagat
    14881 aaagattgtc tttcattggg cgtacatcac cttcgcttga aatagtatca gataattttg
    14941 acgctccttt atctttagta tcttccttat ctttggtaat acaacctgaa ataacagata
    15001 atactaaaag aattgagata actaaagaca aatttttaat gcattttttt aacattttat
    15061 tgcctcctta ttatattatt ttaaaactat actgaagttt tacatttatt tattaactct
    15121 atcattaacg atagagtaat aaccttgtgt cgcacctaac ccttaataga accaatcatt
    15181 atgcctttag cgaaatactt ttttataaga ggtaatacta caacgatagg taagcatgcc
    15241 gctataacaa tgacatattt tatagattcc gaaatttgct gcattaaaac tgacagcaaa
    15301 gcatccgatt cttctgattc catttgtttt gcagttattt gagaccgcag taaaatatct
    15361 ctaagtataa gttgtaatgg atatttttta gggttatata tatataataa cgcgctccca
    15421 taagcattcc aatgttctat tccatattgc aaggctaata ttccaatgat tgctcccgat
    15481 attgggatta caatatttat gaaaaatttg aaattaccac acccatcaat taaggcagaa
    15541 tcaaaaagct catccggtat agtagtttga aaaaaagttc ttgctagtat taattttgat
    15601 gttgaaattg ctccgggtaa cactaaagcc catattttat ctaataagcc caattgtttt
    15661 acaacaataa aattaggtat catacctcca ccaaaaaaca tagtaaatat tataaatata
    15721 gttaatggtt ttttcaactt gaaatcatct cttgatagca cgtatgcagc aggaagtgtc
    15781 atcaccagat ttactattac accgacaaca gtataaattg tactattaag atatcctgtt
    15841 tttaggcgtt catctcgtaa tacgcgttga aaccctaaaa aattatttcc ctttggtaaa
    15901 agaacaactt gaccactcgc tacagctgat ggagaactga tagacgcaat tacaacaaaa
    15961 tacatagggt aaaatacaat aatagcaaaa atagttaaaa gtactatatt aaaaaaatca
    16021 aacacatcat caccactaat accattttcg gcaatatttc taattctcga cttccgcttc
    16081 tcgtttttca ttcttattct catccttttc tccataaatc ttttcttttt ctccttttac
    16141 cataaactac tttcggatac ttgtctggat acaaaattag ctattagtaa taatataaaa
    16201 ttaacaactg agttaaataa accaattgct gctgaatatg agtattgcat acctataata
    16261 ccaaccttgt aagtatatgt tgatataatt tcacttactt caatattaaa aggagtctgc
    16321 aggagataac tcttttccca accaagactc ataagtcccc ccatcttaag tataagttgt
    16381 attacaaata cttttgcaat aatcggaagc tcaatattaa ttatctgcct aaactttgat
    16441 gctccgtcta ttctggcagc ttcatataac tctggattta ttcctgtaag agcgcctgta
    16501 tatataagtg aactccaacc ggcatattgc agtatgcctg gaataacata aacataagca
    16561 tatagctccg gtttattaag aaaatctatt acttctatcc ctaatttagt taaaataatg
    16621 ttaacaatgc ctgtataagg atttaaaaag attcttatca ttgcaactgc tataactgtt
    16681 gaaatgaatt gtggaacgta tgttacagct tgaattctgt tttttgtttt ttccttgttt
    16741 agtgaattta gcatcaatgc caacaaaaca ggaactggtg tatataacat taggtatgta
    16801 agatttatcc gtaaagtatt ttttatgtac ataaaagcaa ttggcaaaga aaaaaagttg
    16861 cgaaaatgtt ttaatccaac aaacggactt tttagaaagc ctaaaacaat gttataatct
    16921 ctaaatgcca tttgtattcc gtataacggc caataatgaa atattatgta atacaagatc
    16981 attggcaagg ccattagata gagatcataa ttctttatga gttcttttct tattcgacct
    17041 tttgtcttca tat
//
LOCUS       DAOSDD010000083        16718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1401_length_16718_cov_5.141631, whole genome shotgun sequence.
ACCESSION   DAOSDD010000083 DAOSDD010000000
VERSION     DAOSDD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_16718_cov_5.141631"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PK733_08970"
     CDS             <1..1205
                     /locus_tag="PK733_08970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ10709.1"
                     /translation="LPIILSLLIAFIPAFTSSSYIYGVTEYDAETVAKIMKDTDVFSL
                     KCKASILMDSDTGTIMIENNSHEKLPIASITKIMSMYLIMEAIENGKLKFDDFVPVSE
                     YSYGFGGSQVYLKPGEEFTVHEMLKAIAIHSANDATVAMAEKVAGSEETFVAIMNEKA
                     KELGLKDTHFLDCTGLTNEGHYSTAYDIAVLSRELIAKYPKILDYTKIWHDTFRNGEF
                     SLDNTNKLVRHYSGTVGLKTGFTSIAGHCLSAVVKRENLTLISVVLGGPDSNTRFAET
                     RKLMDYGFANFESIVLENKDTEAGNIEVRKGIETEVKGILASDVIVLLKKGTKQNIER
                     HVNIEPFIIAPVEMGQKVGEIVYKLDGKEIARVNIITEKGVDKASFMKLFFRMVLEWF
                     GIGKNKSE"
     gene            1334..2047
                     /locus_tag="PK733_08975"
     CDS             1334..2047
                     /locus_tag="PK733_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ10710.1"
                     /translation="MLLRYPITILIYLVPVILISITLHELSHAYCSYRLGDPTAKNMG
                     RLSLNPLKHLDILGTLMILISGFGWAKPVPINPMYYRDRKKGSMLVALAGPLSNVFLA
                     ITFSFPMAFLGSKYGKIPIENMFDINMTLFYSGFDFRVILFNLSRFFYMININLAVFN
                     ILPVPPLDGSKILSGILPQRQYFALMQYENYIGVIFLILIFIFPGFLYKIMFPFIWLL
                     ETAIKSIAVPVLKLLAGVW"
     gene            2212..3210
                     /gene="trpS"
                     /locus_tag="PK733_08980"
     CDS             2212..3210
                     /gene="trpS"
                     /locus_tag="PK733_08980"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ10711.1"
                     /translation="MKNDTIVSGMRPTGALHLGNYFGALYNWIKLQNDYKCLFFVADW
                     HALTTGYEDTSDINNNITSLVTDWLSAGLDPDKCSIFLQSSVKEHAELHLLLSMITPL
                     SWLFRCPTYKDQLAQIKDKNITNYGFLGYPCLMSSDILLYKAKFVPVGEDQLPHLELT
                     REIARRFNYLYGEVFPEPAPKLTEAKVLPGTDGRKMSKSYGNTISLSDSPETVKNKVS
                     TMITDPARIRKNDPGHPEVCTVYAFHKVFNPNDISEIEEQCCGGKIGCVQCKKKLANS
                     MVESLAPIYEKRQEILSKPDYVNDIVQKGNKEARSIAEKTIEEVRSVMKIDWNASK"
     gene            3271..4020
                     /locus_tag="PK733_08985"
     CDS             3271..4020
                     /locus_tag="PK733_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOJ10712.1"
                     /translation="MISSLTNGCKIKVGNFEGPFDLLFHLIEKNKIDIYDIPIGEITD
                     QYMDYLFAMQKMDLELASEFLVMAATLLHIKSRVLLPPEKDKEKEEESDPREELVIKL
                     IEYKKYKEVSLQLQKRETEWSKFYYKSPENIEAKDVKQKLDLNLDNFREAYLNLMKKN
                     RLKENNRTQEIQQILQKDKVSIKSKIKHIMNILLSKKQFVFTDVFPFREKSLTEIVTA
                     FLALLVVVKSGKAAVYQKKQYSDIIVEHQDS"
     gene            4127..4723
                     /gene="scpB"
                     /locus_tag="PK733_08990"
     CDS             4127..4723
                     /gene="scpB"
                     /locus_tag="PK733_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOJ10713.1"
                     /translation="MYLEENNDNNPIDKYENIDEKTNMEEADKIEGIIEGLLFASGVP
                     LHKEKIMELLDIDEGKVVDTLERMNMKYGRNSRGIMIRKINDCYQLCTKPQYSDYIAK
                     LVEPGYKSTLSQAAYETLAIIAYNKPITKAGIEKIRGVNCDSSIAKLLEGNLIREAGR
                     MDSPGRPILYETTEDFLRYFGFSSNDDLYSLKGIADLK"
     gene            4896..5540
                     /locus_tag="PK733_08995"
     CDS             4896..5540
                     /locus_tag="PK733_08995"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOJ10714.1"
                     /translation="MLYPVIFILAAIIISLILFIKVNTKFECVKKSENSDLVIYFFIF
                     NGIIKKKHVIPITDIYTIGNKVYELLLSRYGILKDTDKEYTNLEELLKEYNYLDKAAK
                     LLRLFLTSIQKYLIGGKVPEIKLKINIIYGTDDALWTGVLGGVIWAFAGNIDSLFSNK
                     FKLTEREISIKPDFTKIVLDIEVLCILSIRVVHIIIAGFLFIFELVSEKIKNKR"
     gene            5559..6035
                     /gene="ytfJ"
                     /locus_tag="PK733_09000"
     CDS             5559..6035
                     /gene="ytfJ"
                     /locus_tag="PK733_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOJ10715.1"
                     /translation="MLEHPIEGLMTTAMESIKEMVDVNTIVGDAVQAPDGTVIIPISK
                     VSFGFAAGGGEYSGNNKGGKKDEERNEEGNAENSKFPFAGGSGAGVSINPVAFMVVGN
                     GQIKLLPVNINSSVEKILDLIPELINKANESVKKKIIIKKDIGPDKEHDADTDKKE"
     gene            6149..6856
                     /locus_tag="PK733_09005"
     CDS             6149..6856
                     /locus_tag="PK733_09005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ10716.1"
                     /translation="MDKVRLQKYIAHSGVASRRKAEELIKQGKVEVNGRIVSEPWLMI
                     GNEDSVKVNGKVVSIEKNLIYIMLNKPLGYVTTVKDQFHRKTVLDLVVHVNERIYPVG
                     RLDYDTSGLILLTNDGDFAYKLTHPSHEIKKVYIAEVNGIPDKDDIRKFVGGLEIGSY
                     KTWPAEVCIQEKGKDRSTLAITIHEGRNRQVKKMCEAIGHPVIKLKRIAIGKVNLGSL
                     SEGKWRHLTKEEVESFS"
     gene            6996..7568
                     /locus_tag="PK733_09010"
     CDS             6996..7568
                     /locus_tag="PK733_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ10717.1"
                     /translation="MSTIKNALNQSHDYIKNLVKSGDVVVDATAGNGNDTLMLAKLVG
                     EKGKVYSFDIQEKALENTLRKLIDAGVEERVTVIKADHQYMDTYITEKGQTKAIMFNL
                     GYLPGGDHSISTKPETTIQAVKKAMDLIMPGGLISIVVYYGGDSGFNEKNSIMEFIKT
                     IDPKKYVVMKTEFVNQINCPPILLFIERID"
     gene            7885..9435
                     /locus_tag="PK733_09015"
     CDS             7885..9435
                     /locus_tag="PK733_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ10718.1"
                     /translation="MANNDKINRLREKRECVELGGGQEAIEKQHRAGKKTARERINLL
                     LDEGSFVETDAFVETRSVDFGIQGKKAPGDGVVTGYGTVDGRPVFVAAQDFTVIGGTL
                     GEANAKKIVKVMDMAIKTGAPFISVNDSGGARIHEGINALSGYGDIFLRNTLASGVIP
                     QISLVLGPCAGGACYSPAITDFIFMVEEKGQMYITGNQVVKAVTGEDVSLEELGGALV
                     HNSVSGVAHFKYPSEDSCIKDLKRLLSFLPDNNISGIPSYSCSDDLNRISEDMIDLIP
                     DEPNKPYDMMDVITRVVDNGDFFEIHKYFAQNIIVGFARINGKTIGIVANQPKVMAGS
                     LDINSSDKAARFVRFCDAFNIPLLTFTDVPAFLPGVGQEHNGIIRHGAKLLYAFAEAT
                     VPKVNVIVRKAYGGAYIAMNSKTLGADMVYAWPTAEIAVMGAEGAANIIFRKEIAQAG
                     DPIATRKEKVEEYREKFSNPYAAAARGFVDDVIDPSATRPMVASAFEMLAGKRENRPS
                     KKHGNIPL"
     gene            9521..9952
                     /locus_tag="PK733_09020"
     CDS             9521..9952
                     /locus_tag="PK733_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ10719.1"
                     /translation="MKKFLIKVNGSQYEVEVEEIGKGNFDVEEIHEAASTRTVPAAPA
                     PSPAPAAPKAAAPAAPAKNTTSAAVPAGAQTISAPMPGVINSVNVNSGDSIKKGDILL
                     ILEAMKMENEIISPVDGTIASVNVSKGASVNAGDVLLSINK"
     gene            10035..11432
                     /locus_tag="PK733_09025"
     CDS             10035..11432
                     /locus_tag="PK733_09025"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOJ10720.1"
                     /translation="MAKVQITETVLRDAHQSLIATRMKTEDMLPVIKKLDQVGYFSLE
                     CWGGATFDASLRFLDEDPWERLRKIRDGAKNTKLQMLLRGQNLLGYKHYADDVVEYFV
                     QKAIANGIDIIRIFDALNDVRNIETAIKACKKEGGHAQAAVSYTISPVHDLDYFVKVA
                     KILVDYGADSICIKDMAGLLLPYAAYDLVKALKENVSIPIQLHTHYTSGVASMTYIKA
                     IEAGVDVVDCAISPMAMGTSQPPTEPLVASLKNTPYDTGYDLSLLSEIADYFKPLKEK
                     FVESNLMDIKVMGVDVNTLKYQVPGGMLSNLVSQLKQANKEDKFDDVLKEIPRVREDF
                     GYPPLVTPTSQIVGTQAVMNVISGERYKMVPKESKALVKGEYGKTPAPISKEIQDLIL
                     KGEKPITCRPADLIKPELESIKSQMNEYMEREEDILSYALFPQVAEKYFKYRQAQKYK
                     IDSNLVDYENQTHPV"
     gene            11776..12687
                     /locus_tag="PK733_09030"
     CDS             11776..12687
                     /locus_tag="PK733_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOJ10721.1"
                     /translation="MGRGVGLNKKSYDLLKEIQDKYREFSKGQRAIAEYIINNYDKAA
                     FMTASKLGEVVNVSESTVVRFAVKLGFEGYPELQKMLQDLIKSKLTSVQRIAVSSSRI
                     DSENILKSVLQSDMDKLKITLDNIDQEEFNNVVQCILNARKIYILGVRSSAALASFLG
                     FYFNLIFDNVRLVHTTSVSEMFEQIVRASEGDVVIGISFPRYSKRTIKAMQFAKDKGC
                     KVIAITDSKKSPISEYADYSLIARSDMASFVDSLVAPLSVINALIVAVGMKKERDVYD
                     IFEKLETIWDEYQVYEKYDDIDITPFD"
     gene            12883..14112
                     /locus_tag="PK733_09035"
     CDS             12883..14112
                     /locus_tag="PK733_09035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ10722.1"
                     /translation="MKKRVAVIGGGPAGLMAASKAGERGLDTVVFEKNKVLCKKLMIT
                     GKGRCNITNNTDVEGLIENVPGNGHFLYSSFYTFSNADIIDFFEKHGLKTKVERGGRV
                     FPVSDDAKEVADVLIEYAKRNNVKIMLESSLKEIVVNNNSIKGVLMDNGRMYDCESVI
                     LATGGASYPRTGSTGDGYVIAKKIGHRIIDPRPSLVPLETEEPWVKELQGLTLKNVSV
                     TFKDINGKKLYSDFGEMLFTHFGVSGPLILSGSRHLIDTHIKGSKLVIDLKPALSEAK
                     LYERIGRDFENYSRKMFKNALDDLLPGKLIPIIIMLSNIHPEKPVNQITRDERRGLTL
                     LLKNIVITIKGTRPINEAIITSGGVAINEIDPSTMESKIIKGLYFAGEVIDVDGYTGG
                     FNLTIAFSTGYLAGLNC"
     gene            14144..14662
                     /locus_tag="PK733_09040"
     CDS             14144..14662
                     /locus_tag="PK733_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10723.1"
                     /translation="MKYKSYKIISKPVKKQKGSSFRSILLGINVERFLIVSFFVLFSL
                     LIFTQAILATTGIGKGLAANSNFEGFPLQKEEFLYNEGELVLKLVPIYGQQGEDAKIL
                     VNGEEVGNFADYEYALKLINGDVIEIDANCISDYVDVMVKSKSSNIKLDNLDKKYRLR
                     SEVKKIIKVKID"
     gene            14786..15211
                     /locus_tag="PK733_09045"
     CDS             14786..15211
                     /locus_tag="PK733_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HOJ10724.1"
                     /translation="MDNYLKQYGSKEIATAAIKIALTADRRDEKILQQEFSKSGILTA
                     AVDYGGEFISSVMKIVERAVVSSKREGVIGDSHAEEGAVAGATREALSQIMPKAIGLN
                     VGGKIGIARYNDHISVAIFFGIGLLHLNEVAIGLGHRVV"
     gene            15301..15660
                     /gene="aroH"
                     /locus_tag="PK733_09050"
     CDS             15301..15660
                     /gene="aroH"
                     /locus_tag="PK733_09050"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOJ10725.1"
                     /translation="MSVRAVRGAVTVDNNSSEDVMNETIKLLSRMIDDNVINKDDIIS
                     IIFSTTHDINAAFPAAAARKMGWTDIPLMCTNEMDVPGSLPKCIRILMHINSDKTNAQ
                     LRHIYMKDARKLRPDLT"
     gene            15774..16445
                     /gene="cmk"
                     /locus_tag="PK733_09055"
     CDS             15774..16445
                     /gene="cmk"
                     /locus_tag="PK733_09055"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ10726.1"
                     /translation="MINIAIDGPAGAGKSTIAKAVSKRLDIIYLDTGAMYRTVAFKAI
                     KEGIGCLERGKLSEIVSNINMKIEFLDNEQCMILDNEDVTELIRTPEISIGASNVAVI
                     PEVRIKMVELQRKFASENDVVMEGRDIGTYVLPDATVKIFLTASIEERAARRYKQLVD
                     KGDPKISLDGVIKDIEFRDKNDSSRNFAPLIKAKDAIEIDTTFLSIEQVVDKIIQNVR
                     ESINY"
     gene            16487..>16718
                     /locus_tag="PK733_09060"
     CDS             16487..>16718
                     /locus_tag="PK733_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ10727.1"
                     /translation="MAEFVKFLSRIIFKIFYKFELKGIENVPAEGPAILCSNHPGTLD
                     MFFIGCKLKRLVHYMAKEELFKNPFLAFILPKL"
BASE COUNT         6118 a         2326 c         3338 g         4936 t
ORIGIN      
        1 agcttcctat aatactaagt ttgctaattg catttatacc agcatttaca tcctcatcat
       61 atatttatgg tgtaactgaa tatgatgctg agacagtagc aaaaataatg aaggataccg
      121 atgtatttag cttgaaatgt aaagcgtcta tattaatgga tagtgatact ggaacaatta
      181 tgattgaaaa taacagccac gaaaaacttc ctatagccag tattacgaaa attatgtcca
      241 tgtatctcat aatggaagct atagaaaacg ggaaactgaa atttgacgat tttgtccctg
      301 tatctgaata ttcatatggt tttggaggct cacaggtgta tcttaaacct ggagaagaat
      361 ttactgtcca cgaaatgtta aaggcaattg caatacattc cgccaatgat gctactgtcg
      421 ccatggcaga aaaagttgca ggcagtgaag aaacttttgt agctataatg aatgaaaaag
      481 cgaaggaact tggcctgaag gacacccatt tccttgattg cacaggcctt acaaatgaag
      541 gacattacag cactgcatat gatatagctg ttttgtcaag agagttaatc gcaaagtatc
      601 ccaagatact tgattataca aaaatatggc atgacacatt cagaaatggt gaattttcac
      661 ttgacaatac aaataaactt gttaggcatt attcgggtac agtaggctta aaaacaggat
      721 ttacttctat agcaggtcat tgtctttcag cagtagttaa aagagaaaat ttaactttga
      781 tttctgttgt actgggaggg ccggactcaa atacaaggtt tgctgaaaca agaaagctga
      841 tggattatgg ttttgcgaac tttgagtcaa ttgtattgga aaataaagac actgaagcag
      901 gtaatattga ggtaaggaaa ggtattgaaa cagaagtaaa gggaatactg gcaagtgatg
      961 taatagtttt gctaaaaaaa ggtactaaac aaaatattga acgtcatgtg aatattgaac
     1021 cttttattat tgctccggta gaaatgggtc aaaaagttgg tgagatagtt tataagctgg
     1081 atggaaagga aattgcaaga gttaacataa taactgaaaa gggtgttgat aaagcatcat
     1141 tcatgaaact gttttttagg atggttcttg aatggttcgg tattggtaaa aacaaatcag
     1201 aataataatg gaataataat tctgtagttt cttcatttct tctgctataa tgatttatat
     1261 gaatatgaat aaatactcat tatgatcagg gagacaggca ctctgattta tagtaaataa
     1321 aggagagata aatttgctat tgagatatcc tataactata ctgatttacc ttgtaccggt
     1381 aatattaata tcaattactc ttcatgagct gtcacatgca tattgttctt acagattagg
     1441 ggatcctact gcaaaaaaca tgggaaggct atctctaaat ccgttaaaac accttgatat
     1501 tttaggaaca ttgatgattt taatctctgg gtttggatgg gcaaaaccag taccaattaa
     1561 ccccatgtac tatcgtgata gaaaaaaagg ttcaatgctt gtagctcttg caggacctct
     1621 atcaaatgtt tttcttgcca taactttttc ttttcctatg gcttttctgg gaagtaaata
     1681 tggaaagatt cctattgaga atatgttcga tattaatatg acactttttt actctggttt
     1741 tgattttaga gtaatacttt ttaatttaag ccgttttttc tatatgatta acataaatct
     1801 tgcagtattc aatatattgc ctgtaccacc tctggatggt tccaaaatac tttcgggaat
     1861 acttcctcag agacaatact ttgcccttat gcaatatgaa aactatatag gagtaatatt
     1921 tcttatttta atatttattt ttccaggatt tctatataaa attatgtttc cttttatatg
     1981 gctgttggaa acagctataa agtctattgc tgttcctgtg ttaaagttat tggcaggtgt
     2041 atggtaatca gttaatagga aacatacccc atacagataa atagattgac aaacatctgt
     2101 aatatatcta aaataaatta atactactgt gtcaatttag gaagcgatgt cttccaggat
     2161 aatttttgat agcatcattc atcagtgtga tgagatagga gagtgaaaat attgaagaat
     2221 gacactattg ttagcggtat gagaccaaca ggagcactgc atcttggtaa ctacttcggg
     2281 gctctttata attggataaa attgcaaaat gattacaaat gcctattttt tgttgccgat
     2341 tggcatgcat taactacagg atatgaggat acatcagata ttaataataa tataactagc
     2401 cttgtaacag attggctgag cgcaggcctt gatcctgata aatgctcaat atttctgcag
     2461 tcttctgtca aagagcatgc tgaactgcat ttgcttttat ccatgataac acctttgtca
     2521 tggcttttca gatgtcctac atataaggac cagcttgctc aaataaagga caagaatatt
     2581 acaaattatg gattcctagg atacccgtgc cttatgtcat ctgatatttt actttataaa
     2641 gcaaaatttg tacctgtagg tgaagatcaa ctgccgcacc ttgagttgac cagggaaatt
     2701 gcaagaagat ttaattacct gtatggtgag gtgttccctg aacctgcacc gaaattaacc
     2761 gaggcaaagg tgctgccggg tactgatggg agaaaaatga gtaaaagcta tggaaacacc
     2821 atatctcttt ctgacagtcc cgaaacagtt aaaaacaagg tgagtacaat gattacggat
     2881 cccgccagaa ttcgcaaaaa tgacccggga catccggagg tatgcactgt atatgcattt
     2941 cataaggtat ttaaccccaa tgatatctct gaaatagaag aacaatgttg cggaggcaaa
     3001 ataggctgtg tccaatgtaa gaagaagctt gcaaatagca tggtagaatc tcttgctcca
     3061 atttacgaaa aaaggcagga aatattatca aaacctgatt atgtaaatga tatcgttcaa
     3121 aagggaaata aagaagcacg aagcattgct gaaaaaacca ttgaagaagt aagaagcgtc
     3181 atgaagattg actggaatgc aagcaaataa cctacagaag ataaaaagat ggtaaaaaga
     3241 ggttaaacca gtaatattga gggagttaca atgattagtt ccttaacaaa tggctgtaag
     3301 ataaaagttg gtaattttga aggccctttt gatctgttat ttcatttaat agaaaagaat
     3361 aaaatagaca tttatgatat accaataggt gaaataacag atcaatatat ggattatctt
     3421 tttgccatgc aaaaaatgga cctggaactt gccagtgaat ttcttgttat ggctgctacc
     3481 ttattgcaca ttaagtcaag ggttcttctc ccgcctgaaa aagataagga gaaagaagag
     3541 gaatcagatc ctagagaaga acttgtaata aagcttatag aatataagaa atataaggaa
     3601 gtatcattgc agcttcaaaa aagggagaca gaatggtcca aattctatta taagagccct
     3661 gaaaatatag aagcaaaaga tgtcaaacaa aaactggatt taaatttgga taacttcagg
     3721 gaagcatact tgaatttaat gaaaaaaaac agactcaaag aaaataatag aactcaggaa
     3781 attcaacaaa tattacaaaa ggataaggta tcaataaaaa gcaaaataaa gcacataatg
     3841 aatattctat taagcaaaaa gcaatttgtt tttactgatg tatttccttt tagagagaaa
     3901 tctttaactg aaattgtaac tgcatttcta gcactgcttg tagtggttaa atcagggaaa
     3961 gctgctgttt accaaaagaa acagtattcg gatattattg ttgagcatca ggattcataa
     4021 cccttgatat gcaaaacaaa gtttacaaca ttgcgtatac aagcattaga tacattgcaa
     4081 atgaaactta tacattacta ataaacttgt tgggagcgtt cttcaaatgt atttggaaga
     4141 aaataatgat aataatccaa tagacaaata tgagaatata gatgaaaaaa caaatatgga
     4201 agaagcagat aaaatagaag gtataataga agggcttctt tttgcatccg gtgtaccgct
     4261 gcataaagag aagataatgg aattactaga tatagatgaa ggaaaagttg tagatactct
     4321 ggaacgaatg aatatgaaat atggcagaaa cagccggggt attatgataa gaaaaataaa
     4381 tgattgctat caattatgca cgaaacccca atactctgat tatattgcca aactggtaga
     4441 acccggatat aaatccacat tatcacaagc tgcctatgaa actctagcta ttattgcata
     4501 taataaacca ataacaaaag caggaattga aaaaataaga ggagtaaatt gtgacagttc
     4561 aattgcgaaa cttctggaag gtaatcttat cagggaggca gggcgcatgg attctcccgg
     4621 aagacccata ttatatgaga ctacagaaga ttttttaagg tactttggct tttcttcaaa
     4681 tgacgattta tattcattga aaggtattgc tgatttaaaa tagtatattc atttttgcga
     4741 gatcaatttt gcccggagct gaatttaaag gctctaggca gtgtaacgtc agcttctggt
     4801 aaatatttct tcaaaaattt taaatgcaca cttcatttag caataatgtg taatgtgaat
     4861 attatttgga tataataacc tgatgaggtg tatttatgct ttatccggtt atttttattt
     4921 tagctgccat tattattagc ttaatactat ttatcaaagt aaatactaag tttgaatgtg
     4981 taaagaaaag tgaaaattca gatttagtaa tatatttttt catttttaac ggaattatta
     5041 aaaaaaagca tgttattcct ataactgata tttatactat aggaaataaa gtttatgaac
     5101 tgctattaag tagatatggt atattaaaag atacagataa agaatataca aatttggaag
     5161 aattattaaa agaatataat tacctggata aagctgcaaa attattaagg ctttttttaa
     5221 caagcatcca gaaatatctg attggaggaa aggttccaga aatcaagctg aaaattaaca
     5281 tcatctatgg cacagatgat gcactttgga caggtgtttt aggaggtgtt atatgggcct
     5341 ttgcaggaaa tatagattca ttattttcaa ataaatttaa acttaccgaa agagaaatat
     5401 ctattaaacc cgattttact aaaatagtac ttgatataga agttttatgc atactaagta
     5461 ttagagttgt ccatattata atagcagggt ttttattcat atttgaacta gttagtgaaa
     5521 aaataaaaaa taaaagatag aataaggcgg tgttttttat gttggagcat cctattgaag
     5581 ggcttatgac aactgcaatg gaaagtataa aagaaatggt tgatgtaaat accatagttg
     5641 gggatgctgt tcaagctccg gatggaacgg taataatacc aatatccaaa gtaagtttcg
     5701 gattcgcagc aggtggtggt gagtatagcg gtaataataa aggaggtaaa aaagatgaag
     5761 aaaggaatga agaaggaaat gctgaaaaca gtaaatttcc atttgccgga ggaagtggcg
     5821 caggagtaag tattaatcct gtagcattta tggttgtagg aaacggtcaa atcaaactac
     5881 ttcctgtaaa tattaactcg tctgtagaaa agatactgga tttgatacct gaattaatta
     5941 acaaggctaa tgaaagtgtt aagaaaaaaa taataatcaa aaaggatata ggcccagaca
     6001 aagaacatga tgcggatact gataaaaaag aataaattca catttttatg gttcgacgaa
     6061 aaataacggt attaactgca tgtacaaaat gatataatat attttactta gtattttata
     6121 tatattgaaa agaattggtg ggtcaactat ggataaagta aggttgcaaa agtatattgc
     6181 ccattccggt gttgcatcac ggagaaaggc ggaggagtta ataaaacagg gaaaagttga
     6241 agttaacggc aggatagtat ctgaaccatg gctaatgata ggaaatgagg atagcgtaaa
     6301 agtcaatgga aaagttgttt ctattgaaaa gaatcttata tatattatgc ttaacaaacc
     6361 tttaggatat gtaacaaccg ttaaggatca atttcacaga aaaactgtgc tggatttggt
     6421 tgtgcatgtt aatgaaagga tctatcctgt cgggagattg gattatgata catcaggtct
     6481 tatcttattg accaacgatg gagattttgc atataaactg actcacccaa gccatgagat
     6541 aaaaaaagta tatattgcag aagttaatgg tattccggac aaagatgata ttagaaagtt
     6601 cgtaggtgga ctggaaattg gctcctataa gacatggcct gcagaagttt gtatacagga
     6661 aaagggaaaa gatagatcaa cactggcaat aacaattcat gaaggtagaa accgccaggt
     6721 aaaaaaaatg tgtgaagcca tagggcatcc ggtaattaaa ttaaaacgca tagcaatagg
     6781 aaaagttaat ttaggttctc tatctgaagg taaatggagg catcttacaa aagaagaagt
     6841 tgagagcttt tcttagcaat acaaaaatgc aatacaaaaa ggtatttccc agtatttacc
     6901 aagtaaaagg cactaaataa gaagtgggag gtgagagaac ataacctctc attctttcat
     6961 gctttgctaa taatagaaag tgaggttgtg cgtaaatgtc tacaattaaa aatgctctta
     7021 atcaatccca tgattacata aaaaacctgg tgaaatcggg tgatgtggtt gttgatgcta
     7081 ctgcaggtaa tggtaacgat actttgatgc ttgcaaaact tgtaggggag aaaggcaagg
     7141 tatattcctt cgatatacag gaaaaagcct tggaaaatac attgagaaaa ttgattgatg
     7201 caggggttga ggaaagagta actgttataa aagccgatca tcagtacatg gatacatata
     7261 taactgaaaa aggacaaaca aaagctataa tgtttaatct gggatatctt ccgggtggag
     7321 atcatagtat ttcaacgaaa cctgaaacca ctattcaggc agttaaaaaa gcaatggacc
     7381 ttatcatgcc gggcggactt attagtatag ttgtgtatta tggcggtgac agcggtttca
     7441 atgaaaaaaa cagtattatg gaatttataa agacaataga tcctaaaaaa tatgttgtaa
     7501 tgaaaacaga gtttgttaat caaataaact gtcctcctat actattattt atagagagaa
     7561 tagattgaaa ggcttgaaag gcttgaaagg cttgaaaacc attttccctt tcaatccagc
     7621 atgagtggta attaaatgca ccaataaatg attttttaat atgcttgata ttatgatata
     7681 acaggcttat attacataat aactaaatat tggcctgtaa aattatctat aaataacatt
     7741 tgcatattat tttacattat attataataa tttcatatgc gctttgttaa ataactatca
     7801 aatagcaact tgagattaca taaagtaaca ctaataaatt gaataagtaa aataaattat
     7861 aaagtatata aggtgggaaa atttatggca aacaatgaca aaataaaccg gctgagggaa
     7921 aaaagagaat gcgttgaact tggtggagga caggaagcca tagaaaagca gcatcgggca
     7981 ggtaaaaaaa cagctcgaga aagaataaat cttcttcttg atgaaggcag ctttgttgaa
     8041 actgatgcat ttgttgaaac ccgcagtgtg gattttggga ttcagggaaa gaaagcaccg
     8101 ggagacggcg ttgtaacagg atatggcact gttgatggac ggccggtatt tgtggcagct
     8161 caagacttta ctgtaatagg aggtaccttg ggagaggcaa atgcgaaaaa aattgtaaaa
     8221 gtaatggata tggctattaa aacgggagca ccttttatta gcgttaatga ttccggcggt
     8281 gccagaattc atgaaggaat taatgcttta agtggatatg gcgatatctt tttgagaaat
     8341 actctggcgt caggtgttat acctcaaata tcattagtac tgggaccatg tgcaggtggt
     8401 gcatgttatt ctcctgcaat cacagatttt atatttatgg tagaagaaaa aggacaaatg
     8461 tatattactg gtaaccaggt tgtaaaagct gttacaggtg aagatgtctc acttgaagaa
     8521 cttgggggag cgttggttca caactctgta agtggtgtag ctcatttcaa atatccttct
     8581 gaagatagtt gtataaaaga tcttaaaagg cttttgagtt ttctgccgga taacaacatt
     8641 tcaggtatac cttcttattc atgcagcgat gatcttaaca ggatttcaga ggacatgatt
     8701 gaccttattc ctgatgaacc aaacaaacca tatgatatga tggatgtaat aacaagagtt
     8761 gttgacaacg gggatttctt cgaaatacat aagtattttg ctcaaaatat tattgtagga
     8821 tttgcccgta taaatgggaa aactataggc atagtggcaa atcagcctaa agtgatggcc
     8881 ggcagtctgg atataaactc atctgataaa gcagcaaggt ttgtaaggtt ctgcgatgca
     8941 tttaacattc cgttattaac ttttacggat gtacctgctt tcctgcctgg tgttggacaa
     9001 gaacacaatg gaataattag gcatggagcg aaattattat atgcttttgc agaggcaaca
     9061 gtaccaaagg ttaacgtaat agtaagaaaa gcctatggcg gagcttatat tgcaatgaac
     9121 agcaaaacac ttggggcgga catggtatat gcatggccta cagctgaaat tgcagtaatg
     9181 ggagcagaag gtgctgcaaa tataattttc aggaaagaaa ttgcccaggc gggagatcct
     9241 attgcaacga gaaaagaaaa ggtggaagag tacagagaaa agttttcaaa tccatatgct
     9301 gctgctgcaa gaggttttgt tgatgacgta attgatcctt cagcaaccag acctatggtt
     9361 gcaagtgcat ttgaaatgct tgcaggtaag cgcgaaaaca gaccttccaa gaagcatggt
     9421 aatataccac tgtaatgtat gcacagcaga cagtaataat tggtcatatt ttataatttg
     9481 aaactttaca ataattgaca atagttcagg aggatttatt atgaaaaagt ttttgataaa
     9541 agttaatggt tcacaatatg aggttgaagt tgaagaaatt ggtaaaggaa attttgatgt
     9601 agaagaaata catgaagcag caagtacaag aactgttcca gcagctccag ctccttcacc
     9661 ggctcctgca gctccaaaag cagcagctcc cgccgctcct gcaaaaaata caacaagtgc
     9721 ggcagtacct gccggagcac aaactataag tgcacctatg cctggtgtta ttaatagtgt
     9781 aaatgtgaat tcaggtgatt ctataaagaa gggtgacatt ttacttattc ttgaagccat
     9841 gaaaatggaa aatgagatta tatcacctgt agatggtaca atagcatctg taaacgtttc
     9901 aaagggtgca tcggtaaatg caggagatgt gcttttgtct ataaacaaat aaaatattta
     9961 ttgtataaag agacatacaa atattgtttt taaaagttaa aagcacatat attttgaaag
    10021 gagtatgttt aataatggct aaagttcaga taacagaaac agtattaaga gatgctcatc
    10081 agtcccttat agctaccaga atgaagacag aagatatgtt gcctgtaata aaaaagctgg
    10141 accaagtagg gtatttttca ctggaatgtt ggggtggtgc aacctttgat gcatctttaa
    10201 gatttttgga tgaagatccc tgggaaagac ttagaaaaat aagagatgga gcaaaaaata
    10261 ccaagcttca aatgcttcta aggggtcaaa accttcttgg atacaaacat tatgctgatg
    10321 atgttgtgga atattttgtt caaaaagcaa tagcaaatgg tattgacatt ataaggatat
    10381 tcgatgctct taatgatgtc agaaatatag aaacagctat aaaagcatgt aaaaaagaag
    10441 gaggacacgc gcaggctgca gtgtcttata caataagtcc tgttcatgac cttgactatt
    10501 ttgtaaaagt agctaaaata cttgttgatt atggtgcgga ctctatctgc attaaagata
    10561 tggcaggcct ccttcttcca tatgcagcat atgatcttgt aaaagcttta aaagaaaatg
    10621 tttcaatacc aatacaactg catactcatt acacaagtgg tgttgcatcc atgacttaca
    10681 ttaaagcaat tgaagcaggt gttgatgtag tggactgtgc aatatcacct atggccatgg
    10741 gtacatcaca acctcctaca gagcctctgg ttgcatcatt aaagaatacc ccttatgata
    10801 caggatatga tctttctttg ttaagtgaaa ttgctgatta tttcaaacct ttaaaagaga
    10861 aattcgtaga aagtaattta atggatatta aggtaatggg agttgatgta aatacactta
    10921 aataccaggt tccgggtggt atgctttcaa accttgtatc acagcttaaa caggcaaata
    10981 aagaagacaa atttgatgat gtgctaaaag aaataccaag ggtccgcgaa gatttcggat
    11041 atccgccgct tgtaacccct acaagccaga tagtaggaac ccaggctgta atgaatgtta
    11101 tcagcggaga aagatataaa atggtaccta aggaatcaaa agctcttgta aaaggagagt
    11161 atggaaaaac tccagctcca atttcaaagg aaatacagga tttaatatta aaaggtgaaa
    11221 aaccgattac ctgcagacct gcagacctta ttaaacctga acttgaaagc attaaaagtc
    11281 aaatgaatga atacatggaa cgggaagagg atatattatc ctatgcctta ttcccacagg
    11341 ttgcagaaaa gtattttaaa taccgtcagg cacaaaaata taagatcgat agcaatttag
    11401 tagattatga gaatcaaacc catccggttt aagcaattaa ccattgaaca aaaaattgac
    11461 ggaatttgat gaaagttgaa tagttctgaa tttaaaaaat tttaaaaata cccaccttct
    11521 aaaaggtggg ttatttttaa ataatatagc caatttgcaa tataacaaag tagcatgcaa
    11581 tttagcaacg aaaatcaaat taaaggctct aggcaccgta acgacagccc ctggtaaata
    11641 tttcttcaaa tttagaaaat gtacccgtgc atgttatatt tctacaaaaa ttaaaaatgc
    11701 acccaaaaat aaaaataagt atttggttta tctctgaaaa atggtaaaat aaaaatatac
    11761 taaactatac taaagatggg gcggggagta ggattgaata agaaatctta tgatttactt
    11821 aaagaaatac aggacaaata cagggaattt agtaagggcc aaagagctat tgccgaatac
    11881 attataaata attatgacaa agcggctttt atgacggctt ccaaacttgg tgaagtagta
    11941 aatgtaagtg aatcaacagt agtaagattt gctgttaaat tgggatttga aggctatccc
    12001 gaacttcaaa aaatgctgca ggatcttatt aaaagcaaat tgacttctgt tcaaaggata
    12061 gctgtatctt caagcagaat agattcagaa aatatcttaa aaagtgttct gcaatctgac
    12121 atggataaat taaaaattac tcttgataat attgatcagg aagaatttaa taatgtagtc
    12181 cagtgtatac taaatgctag aaaaatatat attttaggag ttagaagctc agctgctctt
    12241 gctagttttt taggatttta tttcaacttg atatttgata atgtgagatt agtccataca
    12301 accagtgtaa gtgaaatgtt cgaacaaata gtcagagctt ctgaaggaga tgtggttata
    12361 ggaataagtt tcccaagata ttccaaacga acaattaaag ctatgcagtt tgctaaggat
    12421 aaaggttgta aagtaatagc aataactgac agcaaaaaat ctcccatatc cgaatatgca
    12481 gattattcac ttattgctag aagtgatatg gcttcttttg tagactcact ggttgcacca
    12541 ttaagcgtta ttaatgcact cattgtagca gtaggaatga aaaaagaacg tgatgtatat
    12601 gatatatttg aaaaattgga aacaatatgg gatgaatacc aggtatacga aaaatacgat
    12661 gatattgata ttaccccttt tgattaagtt atggcaaatc gcagctaagc tgcaagcctt
    12721 cgaagcatga aaatcgctgc gataaattaa ggtacaatat ttcctcaaaa agtgccatga
    12781 agtgccatgt aaggcttacg caacatagct tagtgcgtaa tagcttgtat ttttattaac
    12841 tttttgatga aaatagcgta taaaggagtg tgcattttac cattgaaaaa aagagttgct
    12901 gttattggcg gaggtccggc gggcttaatg gcagccagta aagcaggtga aagaggactg
    12961 gatactgttg tttttgaaaa aaataaagtt ctttgcaaaa agttaatgat aacaggtaaa
    13021 ggaagatgca atataacaaa taatacggat gtagaaggtt taattgaaaa tgtccctggt
    13081 aacggccatt ttttatactc ctcattttat acattttcta atgcagatat aattgatttt
    13141 tttgaaaaac atggtttaaa aaccaaggtt gaacggggag gaagagtttt ccctgtttct
    13201 gatgatgcta aggaagttgc tgatgttctt atagaatatg caaaaagaaa taatgtaaaa
    13261 attatgcttg aatcttcact taaagaaatt gttgtaaata ataattctat aaaaggtgta
    13321 ttaatggata atggcagaat gtatgattgt gaatctgtca ttttagctac tggtggagca
    13381 tcataccctc gaacaggttc tacaggggat ggatatgtga ttgcaaaaaa aattggacat
    13441 agaataattg atcccagacc ttccctggta ccccttgaaa ctgaagaacc atgggtaaaa
    13501 gaattacaag gccttacact gaaaaacgtt tcagttactt ttaaagatat aaatgggaaa
    13561 aaactatact ctgatttcgg cgaaatgctt tttacacatt ttggagtttc aggtccatta
    13621 atattaagcg gaagcagaca tctaattgat actcatataa aaggcagtaa actggttata
    13681 gatttgaaac ctgctttatc tgaagcaaaa ctatatgaac gaataggtag ggattttgag
    13741 aattactcaa gaaaaatgtt taaaaatgct ttggatgatt tattgcctgg gaaattaata
    13801 ccaataatta ttatgctttc caatatacat cccgaaaaac ctgttaatca aataacaagg
    13861 gacgagagaa gaggtttaac attactgtta aaaaacattg ttattactat taaaggtaca
    13921 agaccaatta atgaagcaat aataacttca ggtggagtag caataaatga gattgatcct
    13981 tctactatgg aatcaaaaat aataaaagga ctctattttg caggtgaagt aatcgatgta
    14041 gatggttata caggtggttt taaccttacc attgcttttt ctaccggata tttagccgga
    14101 ctaaactgtt aagataacaa caaagaaagt aggagtttac ataatgaaat ataagagtta
    14161 taaaattatt agtaaacctg tgaaaaaaca aaagggtagt tcttttagaa gcattcttct
    14221 tggaataaat gtcgagaggt ttcttatagt ttcatttttt gtcctttttt cattgctgat
    14281 atttacccaa gccatacttg caaccacagg aattggaaag ggattggctg caaatagtaa
    14341 ctttgaagga tttcctcttc agaaagaaga atttttgtat aatgaggggg aattagtatt
    14401 gaaattagta cccatttatg gacagcaagg agaagatgct aaaatattag taaatggaga
    14461 agaagttggt aatttcgcgg attatgagta cgctttaaag cttataaatg gagatgtaat
    14521 cgaaatagat gcaaactgca ttagcgatta cgtagatgtt atggttaaat ctaaaagctc
    14581 caatataaaa ttagacaact tagacaaaaa atataggtta agatctgaag taaaaaaaat
    14641 aattaaagta aaaatagatt aatttgttgt ttttagattc ttaatcttat ataataagaa
    14701 ctgttatata aaaataacga aaaagttatg ttattgttgc aggtatgatt ccttataata
    14761 ctaataaagg atgtataata atttaatgga taactattta aaacaatatg gtagtaaaga
    14821 aatagctaca gcagctataa aaattgcctt aactgcagac agacgcgatg aaaaaatatt
    14881 acaacaggaa tttagcaaaa gtggtatatt aactgcagcg gtcgattatg gaggagaatt
    14941 tatttcatct gtgatgaaaa ttgttgaaag agctgtagtt tcctcaaaaa gagaaggagt
    15001 cataggtgat agtcatgctg aagaaggcgc agtcgcaggt gccacaagag aagcattatc
    15061 gcaaataatg cctaaagcca ttggtttgaa tgttggtggg aaaataggta ttgcaagata
    15121 taatgaccac attagtgtgg cgattttctt tgggattgga ttattgcact tgaatgaagt
    15181 tgccatagga ctagggcatc gtgtagtata agcaaggcaa ttaatatgta tagtgttgta
    15241 tgagcatata aagcagtata acagtattgt aaaaccaata tactaaaagg aggaatagct
    15301 ttgtctgtta gggctgtaag aggtgctgta acagttgata acaattcgtc tgaagatgta
    15361 atgaatgaaa ctattaagtt attaagcagg atgatagatg ataacgtcat caataaagat
    15421 gatattatta gtataatatt ctcaactact catgatatta atgcggcttt tccggcagca
    15481 gcagccagaa aaatgggatg gacagacatt cccctgatgt gtactaatga aatggatgta
    15541 cctggaagtc ttccaaaatg tattagaatt ttaatgcata taaactctga taaaacgaat
    15601 gcacaattaa ggcatatata catgaaggat gccagaaaat tacgtcctga tttaacttaa
    15661 ataataagtt attaatagat caaaatagac gcatattatt tttataaaga aattgtagga
    15721 ggaagcattt aaaaattatt aacatttgat atattgctgg aggtttatat ataatgatta
    15781 atatagctat agacggacct gcaggagctg gaaaaagtac tatcgccaaa gcagtttcaa
    15841 aaagactgga tataatatat ctcgatacag gagcaatgta taggactgta gcttttaagg
    15901 caattaaaga gggcattggc tgtcttgaac gcggaaaatt atccgaaata gtaagcaata
    15961 taaacatgaa aatagaattt ttagataatg agcagtgtat gattttggat aatgaggatg
    16021 taactgaact cataaggaca ccggaaattt caattggtgc ttcaaacgtg gctgtaatac
    16081 ctgaagtaag aataaagatg gtcgaattgc agagaaaatt tgcctcggaa aatgatgttg
    16141 tgatggaagg aagagatatt ggaacatatg tactacctga tgcaacagta aagatttttt
    16201 tgactgcttc catagaagaa cgtgcagcaa ggagatataa gcagttggtt gataaaggtg
    16261 atcctaaaat atctttagat ggagtaataa aagatattga gtttagagat aaaaatgaca
    16321 gcagcagaaa ttttgctccc cttattaaag caaaggatgc aattgaaatc gacactacat
    16381 ttttatcaat tgaacaggta gtagacaaaa taattcaaaa tgtcagggaa tctattaatt
    16441 attaaaataa acacataagg ataaagaatt aataaaggaa attaatatgg cggaatttgt
    16501 taaatttctt tcaaggataa tatttaaaat attttataaa tttgagttga aaggtattga
    16561 aaatgtgcct gcagaaggtc cggcaatact ttgttctaac catccgggta ctctggatat
    16621 gttcttcatt ggatgcaaat taaaaagact tgtgcactat atggcaaaag aagaactttt
    16681 taaaaacccc tttttagcat ttattttgcc aaaactcg
//
LOCUS       DAOSDD010000084        16682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1405_length_16682_cov_5.459073, whole genome shotgun sequence.
ACCESSION   DAOSDD010000084 DAOSDD010000000
VERSION     DAOSDD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1405_length_16682_cov_5.459073"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4788)
                     /locus_tag="PK733_09065"
     CDS             complement(<1..4788)
                     /locus_tag="PK733_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ10728.1"
                     /translation="MNKKSDKKLFLRLTAIYIALVMMFEVAVMTPVQAIGFQPIASKI
                     VSGVESNMRNSLISDTIVRNPLTASMLESTDTDSGEEEYREIALPVIIDMKNSEDNTL
                     PTTDDNPAGSIEGIKSLSKNYVTAGIPEDGLILEIINPGGGSDSSGGSGSGATGTPAS
                     GALVTVHHAFYNEEEKYMSYEEIWYGYADDDGMVIIPGTEATYGNDFFVTARGFASET
                     SQIETDFIYHATVRAPAKAVIDDSNASVAKISATDLEGQPLAEAKVIIEPLESNVPGF
                     KDPDAILDFFGREDYDNAPILDEEGKASIVLTRGRYNIRIVGRDEVSKDKVSKYYLFE
                     YGVEITSETQKIEFYPTDETVTKLELVSGPDVFDASLALYCTGNLNFGFDMIVPGETI
                     IVTPGTYSAWTDVTYRIPSESPDLPDAGTDWFWSFGNEELKLDAGEPEQYQVGGVINA
                     EIKINEYYEKFYSGDLANFIPHFSDEHGNTITGVYKRSYEKQGNNTVYGDSINVVPQL
                     EIIDSSTGNKYYGISGMDYAYWRIPNDITGSFTASVRLEADPLGPVESDSITFEVVSG
                     SIDPYHLHVNVLDRNGNPMKRARVIPVAQKDSSISYIMSLARITDDNGKASLYLHTYE
                     DYQDYTHGLIIYGNSPDPANPDNPAEPIFMYVPINIEKLPMDLTVNIAEYPMKKVELE
                     SIDVSGEILSVPHYNAIYTKNQNGRTVGAILNNAGYGKQVLWLPYGEYKYQTFINSTD
                     WDDDWQDKPLYFMTKNINIEEQTPETQILKLGGNTLAMLSPGGAQGDKTTPIQALALF
                     GGEGSLSPVFYPNKYDKNESPEVLQKKLYITPGTYKAQGVFTRYDQEDSGNWNFWIEK
                     ELALEDKGNMEWSPGSTYNTEITTYNTSAGINDTVEFENKIVDNAGNRLVAAANNFYF
                     TGWENYLSPNNGTIGIMNHYEIAPFITIFDDNEQEVFRYKNDAGNYSILTKWKQWDIS
                     KQWDISDGAVNSLSEDSTYFKGSYTIPQDLSAGECKANLKLGMGPFGVMEAQTAFAVT
                     GGPGTPVLDQPVTMTNESSITVTGKASPYADIIVYYSLNDGQKTEAASVEASQEGNFQ
                     ADIELSAEGEYKITAVAIVDKIVSNESIPITITVDRTAPGSPMNLGAESPDYSHINLS
                     WEAPESEDISLIKYEIKRDGNVIAKEISGGLSLSYQDSELSEDTEYSYQIIAVDGAGN
                     RSEPAQINIKTVKGPDKIPPEAPKNIKAVLDDESTVTITWDASEDNVATTGYIIYKSI
                     DGSNFTGITRVSVPDELLFTDGELEYNVEYKYQIIAFDLADNKSEAAEITIIIEKPAK
                     LEIISVKWVGSWDNYSHLNAGSNIEVTVVGTAEKTAKVVLEYINSSDVTVTEETILQE
                     INNESEPGIEADAESITATYTGTIAIPDGAKNITKITGSINLDDNKEEMKAEGLPAPV
                     NGGLKVKLSADTLTKLNQLEGAELSLRSKTKNSGGKAIIGTSTEYMITGLIPSGDYEL
                     SLTNGTAYLGDYKNIEIFGGLIKEQEFSFSKSTASLDVRVIDEQGNPFYAASVYVIPV
                     DGTLSAVGYPDMDGWVRQKSARSDILRNPILKNSLEGIVINVSV"
     gene            complement(5069..5419)
                     /locus_tag="PK733_09070"
     CDS             complement(5069..5419)
                     /locus_tag="PK733_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ10729.1"
                     /translation="MRFFDRFKKETRHKNENENNSLQNECVESNYTDNQSVSGLIKQF
                     PFSEDEKKDFVLSLTPREKDIYLLLIEGFTLKEVAKQSGIKYSTVNTHMTQIYKKLKV
                     NTRAELIINYRNTR"
     gene            complement(5632..7080)
                     /locus_tag="PK733_09075"
     CDS             complement(5632..7080)
                     /locus_tag="PK733_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ10730.1"
                     /translation="MKAIFQKTINSFLGTFRQEGVLNINKLTKKNLPFFVSWIAIILW
                     LDFYILPIGTYGEVGKLSAYSPMTIFTFLYPLATTASVFLLDLKKFLPFVKYSAIVGI
                     IGILINTITSNTVILYIGTTITAIAMGNILASIDYGFFMVMNNLERLYSVSIGIFISK
                     IMLVAKVIFADTNYENGIFNVLPLVGFLPILICAWLYGKTTEDERFEPGKKTELRDYT
                     VLILACFVFLFNDFLAPALWRSITSIASLTLNTYHVIGVLLGIVITLFLHLILHCNIC
                     YILNFSFAILTMGFVINAFGSNGEEWILLQVLLFGVSYSMGFVSIYYMMGIIAKKSRS
                     IIFFRVGIFSVAIFYILGSSIISAIKDVNSAALLSAIALISVGIILIIFTLTPLLTKI
                     LHSAEWLDDLYRSDVTHTSRLTARLTELKLTPREIEVCVLLLDGYTMRQISSLINIAY
                     STVNTYCTSIYRKLGINSRTELVVMFSEYAVK"
     gene            complement(7237..7509)
                     /locus_tag="PK733_09080"
     CDS             complement(7237..7509)
                     /locus_tag="PK733_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10731.1"
                     /translation="MVKDVCANCNNNRISYIDSYAKEIIFDYFVRKYEKDDVLDFTYD
                     YTLLQKMLLKYAFNDLRSHKDDTSFFTAPGKYFFTFKICTHVLTLS"
     gene            complement(7561..7740)
                     /locus_tag="PK733_09085"
     CDS             complement(7561..7740)
                     /locus_tag="PK733_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10732.1"
                     /translation="MVYNSNNKKPNNQLLRDDSAGEFIKIGSEAIVICAYCGQEAKGT
                     KEHIISCAILDLFPE"
     gene            complement(7785..8078)
                     /locus_tag="PK733_09090"
     CDS             complement(7785..8078)
                     /locus_tag="PK733_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10733.1"
                     /translation="MIYSVHFYYHKRNSRKAASKFEGIVFAKNQGHAEELVKKLISDY
                     PIEAEEPFSIIGGLDKMLEEIYKERPELIGITPEQGYIYNEFMHRNSISRYVR"
     gene            complement(8275..12879)
                     /locus_tag="PK733_09095"
     CDS             complement(8275..12879)
                     /locus_tag="PK733_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10734.1"
                     /translation="MANEKNGILDKRGENMNWQHFQTYNEAPARAFECMCNQLFELWC
                     KKTYPDSLKAVTIVNGSGGDGGVESFAIKKEDTIIGMQAKWFLNSISANQFGQISNSI
                     KTALRVRPSIKQYIVCIPRDLSSDRIGKGKKVIDNTESNRWQQLKNRLEDDYPGLKIE
                     LWNETQLLTKLQDSDAAGIYRYWFEKSEISKELIVYSYEKQKSGWLSQKYTPCLHIQG
                     QMQKSILKFVGTLDDRQNSISTLSAMSQQCSKFLKACDDYLQFVQDHSSQEQLIHEIQ
                     GTQEQVIKLLGEIKCTIDAVNNDQQVASSINENNWYIDFAMLLELLKQNEQFRGNYFH
                     INEIKKTAEALQENEIHTIISDIRFKLDNKKYLILGNPGSGKTHGIANMVESLLNEKL
                     HIPILIRAKDVNPNNSWIDILKKSLGLASVWNETELWQALEALSYRAEINNVSTADTI
                     VAKIIPKILICIEGVDESTPYELWYDRLREIEAICYKYPRLRFCITSRPYVFRELAYD
                     DSLLSNTLRLPSDGDVPVNELFQEYIEYFRINIEGCSWIKWSLKTPLALRIFCENYRH
                     RSISGVEKSSVTITNLLSKKFHIIEREFNSKYETGYGDREIIVQNSLLAVARKFLINT
                     TVYRNDLIDSLKDVPGLITVAHDYRREFIDFIEDYGILQSYTANSDTILEPPKTYYSI
                     GIQPFFDYILALLITDQIQNPSKVTLTETLTGHEGALQMVSILLLEDYSYLITENKSF
                     RDNLSNNELFDLVCFSLSNVSPNPASKYIPLVKRMMNYNSDCLKSVVNKIVLPVSRIE
                     NHSLGAQILHEYLIGFETAGKRDIIWSVPSYLRYPGDAPWKCYTKIDLSDKRYKLCSE
                     DKYNGIPLVYAWMLTTVNNIERVGYRRELMKWAFTQPFEFYKLFEYTWNTNDPQMKED
                     LFSIAMGTVFMLEKEHPCIKLFSDWMIKNIFASDKISMHYNCAIRYYSRAIVERSYAF
                     GTINDEDVGESRPPYHTSGTIPLNREATSGTRMGGFGPIDYDLARYVLCDPMDRMFFS
                     RRGSDKIAEEIDYTWYFSEKEIEDILTNEEIFLNEKCREKLREIQLAYKKQRKFWDHL
                     DDLIDDGADTTIPNSKSPAYKYNKEADSFLLEHARILEMESLEPNQFVLSAAYAYILL
                     QGWNEEEFYGCPNGGKPGELIGVDIAILRQHHPATHGSKSSIMTFCEKYTWCARNEIL
                     GYLSDRLPFRDFERVPSLLSDYGLLDDFPNPAQELYQENPDSIMEKTSWFIPEELSPL
                     INKDMGEDAIRNWIINAPTPNFEKWIDISDFNHEIVQEEKKNWISLYSFNSVSNALGG
                     ESLMWISSAIITNEQFKYLKNDLINRKDSLVYNLSNPEDLHSSTETQCYITPKEICWM
                     NWKNECYNKIYNISIDDGDFVKYTIDKTVEECTANYPEYGDVYYKLPSRIIRELLDIC
                     DGDGYKYYNGEKQLEAIRFEAGEKWHDSQSYLCVDREKLLLQLEHLNFKIFWTVRLLR
                     QATSKALEKYPKLRSRNDKCWLVWFEDGELRTQLFSEQVD"
     gene            complement(12852..13418)
                     /locus_tag="PK733_09100"
     CDS             complement(12852..13418)
                     /locus_tag="PK733_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10735.1"
                     /translation="MNQHDSWKHFTPNEYESDWRNTDGFLKYCKRINREVGKFPESVL
                     EQSFYKLYNFSGFVDEFSCINYYEVKFELMKLSVDNIMKIKPPKFSNLYLQERIESLK
                     KINGDINRSSYGHLHELINHWYTYGTWKIPIIVMKSSEFNEGNYKLPYQLVEGHNRLS
                     WVQYFANNKSDFKLADSHEIWLMRKMAY"
     gene            complement(13525..14148)
                     /locus_tag="PK733_09105"
     CDS             complement(13525..14148)
                     /locus_tag="PK733_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10736.1"
                     /translation="MDINLSSPNPTLESVFSNPAQVITLDANFLIPPDRSRYAKKSFD
                     FLVFQRIWLEPIFEAFPNLAIHEAVYDELVLPSVRTYIDTNISNSPPRLTIHKDSSLT
                     AEEYMLRNSIEEKIYPLTKYDPLLDNKDDRGEVKSLAYIAVKGLIYFATHDSNTVQLI
                     EKAEEWMTGLDNVQVIKMYELIFYLYKIDKSDNKALRMLYKVLPYVT"
     gene            complement(14151..15023)
                     /locus_tag="PK733_09110"
     CDS             complement(14151..15023)
                     /locus_tag="PK733_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOJ10737.1"
                     /translation="MNHSISLNEIQIENIRKLAGDERRKLGFIGETPIANDIFTILEN
                     MNILLLEYPIKLDDVKPAFSAALMCSTEGDKELVFIGLNTADFFDKQIFAIAHELYHF
                     YTKTGSHLSRLSEEEDSYVEAEANRFAAEFLLPESTLKSIVLDEFKTSSLRKIQTKML
                     LRFIVRLQCVWWLPYRSLVKRLREIDAVSDEQYERLYSIDERDLDGEYGRMGKAINKE
                     VFVKLNTATNNIGTSPKDIEIIIRNFEDGIIDEDKFINTLNLFNKKPDDFGYKFEVSD
                     EDIDEFEEFFNEEG"
     gene            complement(15001..15366)
                     /locus_tag="PK733_09115"
     CDS             complement(15001..15366)
                     /locus_tag="PK733_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10738.1"
                     /translation="MKKLSVAKLAEIVKLKREEKGLTQEQLSDLTGINRVMIGRIERE
                     NFIPSIIQLESLANTLEFDIVDMFIEKQESNSFIALRSETLSESEKEGVEKLFTMMLS
                     LRQQILLRSKFENESLNLS"
     gene            complement(15610..16216)
                     /locus_tag="PK733_09120"
                     /pseudo
     CDS             complement(15610..16216)
                     /locus_tag="PK733_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225161.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            complement(16292..16645)
                     /locus_tag="PK733_09125"
     CDS             complement(16292..16645)
                     /locus_tag="PK733_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10739.1"
                     /translation="MSLLDNSKDSQDFILNNLVKRVEELFGEIDNDIVMNLLHSNDEY
                     SKLYYRRIEIQRQYPAVQKAVDGEGGVSLNNDEHKALVEYLRVNEQANSMEHMEIYFQ
                     GHADCMAYLKKIGIL"
BASE COUNT         4970 a         3340 c         2562 g         5810 t
ORIGIN      
        1 tactgaaaca tttataacta taccttcaag tgagtttttt agtatgggat ttcttagaat
       61 gtcacttctt gctgattttt gcctcaccca accatccata tcaggatatc caacagctga
      121 tagtgttccg tctaccggta taacatatac acttgcagcg taaaaaggat ttccctgttc
      181 atctataacg cgcacatcca gtgatgccgt agatttacta aaactgaatt cctgctcttt
      241 tattaaacct ccgaatatct caatattttt ataatctccc agatatgctg tgccattggt
      301 taaactaagt tcataatctc ctgatggaat taatcctgta atcatatatt ccgttgaagt
      361 acctatgata gctttccccc cggaattttt agttttgctg cgaagagata attctgcacc
      421 ttcaagttga ttcaatttag tcaaagtatc agcagacagt ttcaccttaa ggcctccgtt
      481 aacgggtgcc ggaagacctt ctgccttcat ttcttcttta ttatcatcca gatttatact
      541 acctgttatt tttgttatat tcttcgctcc atcaggaata gcaatggttc ctgtatatgt
      601 tgcggtaata ctttctgcat ctgcttcaat acctggttcg ctctcattat taatctcctg
      661 cagtatagtt tcctctgtta cagttacatc acttgaattt atatattcca gaactacttt
      721 tgccgttttt tcagctgttc caactacagt tacctctatg tttgaacctg cattcaaatg
      781 actataatta tcccatgagc ccacccattt tacagagatt atttcaagtt tagccggttt
      841 ctcaatgatt attgttatct ctgctgcttc cgatttatta tcggctaaat caaatgctat
      901 tatctgatat ttatattcaa cattgtattc aagttctccg tctgtaaata atagttcatc
      961 cggcacagaa actctggtaa ttcccgtaaa attagatcca tctatactct tgtatattat
     1021 atacccagta gttgctacat tgtcctctga tgcatcccat gttattgtta cagttgattc
     1081 atcatctaaa acagccttaa tattcttagg ggcctcggga ggaatcttat caggaccttt
     1141 gactgtttta atgttgattt gtgccggttc agatcggttt ccagcaccat caactgcaat
     1201 aatttgatag ctgtactcag tatcttccga aagttcacta tcctgataac tcaaacttaa
     1261 tcctccggat atttctttag ctattacatt tccatccctc ttgatttcat attttattaa
     1321 gctgatgtct tcgctttcgg gtgcttccca ggacaagttt atatgagaat agtcaggaga
     1381 ttccgctccc agattcatag gagatcctgg tgcagttcta tctacagtta ttgtaattgg
     1441 tatactttcg ttacttacta ttttatcaac tatagcaaca gcagttattt tgtattcacc
     1501 ttctgcgcta agctcaatat cagcctgaaa atttccttct tgagaagcct ctacagaagc
     1561 tgcttctgtt ttttgaccgt catttaggct gtaataaact attatgtctg cataagggga
     1621 tgctttccca gtaactgtaa tgcttgattc atttgtcatt gtaactggct gatcaagcac
     1681 cggtgtccca ggacctccag taacagcaaa agctgtttga gcttccatta ctccaaaagg
     1741 tcccatcccc agttttagat tggctttaca ttcccctgct gaaagatcct ggggtattgt
     1801 atagctgcct ttgaagtagg tactatcttc tgaaagactg tttactgcac catcactgat
     1861 atcccattgt ttacttatgt cccactgctt ccattttgtc aggatgctgt agtttcctgc
     1921 atcattctta tatctgaata cctcttgttc attatcatca aatatagtaa taaagggagc
     1981 aatttcataa tggttcatga taccgattgt tccattatta gggcttaaat aattttccca
     2041 gcctgtaaaa tagaaattat ttgctgcagc aaccagcctg ttacctgcat tatctactat
     2101 cttattttcg aattcgactg tatcatttat tccggctgat gtattatatg tagtgatttc
     2161 cgtattataa gtgcttcctg gtgaccattc catatttcct ttgtcttcca gggcaagttc
     2221 tttttcaatc cagaaattcc agtttccgct atcctcctgg tcataacgag tgaatacccc
     2281 ctgtgctttg tatgtacctg gtgttatata gagctttttc tgaagcactt ccgggctctc
     2341 atttttatca tatttatttg gatagaacac gggcgataaa gagccttctc cgccaaataa
     2401 ggctaaagcc tgaatcggag ttgttttatc tccctgtgca cctcccggag aaagcattgc
     2461 aagggtgttt cctccaagtt ttaatatttg agtttcaggt gtttgttctt ctatattaat
     2521 gtttttagtc atgaaatata gtggtttgtc ctgccagtca tcgtcccagt ctgtagaatt
     2581 tatgaatgtt tggtacttat attctccata tggcagccag agtacctgtt tgccataacc
     2641 tgcattattc aatattgcac caacagtccg gccattttgg ttttttgtat aaatagcatt
     2701 gtaatggggt acagataata tttccccact tacatcaata gattccagtt caactttttt
     2761 cataggatac tctgcaatat ttaccgtgag atccattgga agtttctcta tgttgatagg
     2821 aacatacata aaaatgggtt cagccggatt atcgggatta gccggatcag gagaatttcc
     2881 atagataata aggccatgtg tatagtcttg ataatcttcg tatgtatgca gatacaggga
     2941 tgctttccca ttgtcgtctg taatacgagc aagactcata atataagaga tggatgaatc
     3001 cttttgtgcc accggtatga ctctggctct cttcatagga tttccgtttc tgtctaatac
     3061 atttacatgc aggtgataag gatctatact acctgataca acctcaaatg taatgctatc
     3121 gctttcaaca ggacccagtg gatctgcttc aagccttaca ctggcagtaa aacttccggt
     3181 tatgtcattt ggtatcctcc agtatgcata atccattcct gagattccat aatatttgtt
     3241 tccggtgctg ctatcaataa tttcaagttg tggtacaaca tttattgaat caccgtatac
     3301 agtattattc ccttgttttt catatgacct tttatacact cctgtgatag tatttccatg
     3361 ttcatcacta aagtgtggta taaaattggc taaatctccg gaatagaatt tttcatagta
     3421 ctcattaatt ttaatttctg cattaataac cccacctacc tgatattgtt caggctctcc
     3481 tgcatctaac ttaagttctt cgtttccgaa gctccagaac cagtctgtgc ctgcatctgg
     3541 caaatccggc gactctgatg gaatccgata agtaacgtca gtccatgcag agtaggttcc
     3601 tggtgtaaca attattgttt caccaggaac aatcatatcg aatccaaaat ttaaatttcc
     3661 tgtacagtaa agggcaaggc tggcatcaaa aacatcggga cccgatacaa gctctaattt
     3721 tgtaacagtt tcatctgtgg gatagaattc aattttttga gtttctgaag taatttcgac
     3781 accatattca aaaagatagt atttactcac tttgtcctta ctcacttcgt cccgacccac
     3841 aattcgtata ttatacctgc ccctggtcaa gactatgctg gcttttccct cttcatccag
     3901 tatgggagca ttatcataat cttctcgtcc aaagaaatcc agtattgcat caggatcctt
     3961 aaaaccaggt acatttgatt caagaggttc tataataacc tttgcttccg ccagtggttg
     4021 cccttctaaa tctgttgcag atatttttgc aacagatgca ttactgtcat caataactgc
     4081 ttttgcaggt gccctgacag tagcatggta aataaaatca gtttcaattt gacttgtttc
     4141 agatgcaaat cccctggctg taacaaaaaa atcattccca tatgtagctt cagtaccggg
     4201 tataattacc attccatcat catctgcata accgtaccat atttcctcat aagacatata
     4261 cttttcttct tcgttgtaga aagcatgatg taccgttacc agtgcccctg aagcaggtgt
     4321 acctgttgcg ccgctaccag aaccaccaga actatcagaa ccaccacccg ggttaattat
     4381 ttcaagtatc agtccgtcct ccggaatacc agcagtaaca taatttttcg ataatgactt
     4441 tataccttct atgctacctg caggattatc atctgtcgtt ggtaatgtgt tgtcctcaga
     4501 atttttcata tctattatta ccggcaaggc aatttcccgg tattcctctt caccagaatc
     4561 tgtatccgtc gattcaagca tagaagctgt taaagggttt cttacaattg tgtctgagat
     4621 aagcgaattt ctcatattgg attcaacgcc ggatacaatt tttgacgcaa taggttgaaa
     4681 gcctattgcc tgtaccggag tcataacagc aacttcaaac atcataacta aagcaatgta
     4741 aatagctgtc aaacgtaaaa atagcttttt atctgatttt ttgttcatgg cttactatcc
     4801 ccccattatt atactggtgt tttctatctt catttaaaat catcgcttat agaaattttt
     4861 taacttttat acccgtatat tgcagataga tcataatatt ccatccctga ttcatatgtc
     4921 ccaatgttta tgttactgtt tatattacta tttatattac tgcttatatt actgcttata
     4981 ttactgttta tattactata ttaactactt ttttcattat tgattggtga tactaaaaaa
     5041 ttacattttc catgtcacaa gttattcatt acctggtatt tctatagttg attattaatt
     5101 ctgctctggt gttaactttc aactttttgt aaatttgtgt catatgggta ttcactgttg
     5161 aatattttat gcccgattgc tttgctactt ccttaagagt aaagccttca atcagtaata
     5221 aataaatgtc cttttcccga ggtgttaagc tcaatacaaa gtctttcttt tcatcttcac
     5281 taaaaggaaa ttgctttatc aggcctgata cactttgatt atcagtataa ttcgattcca
     5341 cgcattcatt ttgcaatgaa ttgttctcat tttcattttt gtgccttgtt tcttttttga
     5401 acctatcaaa aaacctcact tacttcacct cctttaatga gctacaagcc tgatttattg
     5461 tatttggtgc tgttgtattt gctgatgtgt ttggtgtcag acgccaaata catcagcatt
     5521 taattctatt ttatgcaaaa tctcctgttt gactattcat tagttctagt gaaaacaaga
     5581 aaagcctcct cattagaact aatgaggagg cttttaaggg tactgtacaa actactttac
     5641 agcatactca ctaaacataa ctactaattc tgtcctgcta tttattccca atttacggta
     5701 aatggatgta caataagtat ttacagtgga gtatgcaata tttattaatg aagaaatctg
     5761 gcgcattgta tatccatcca ggagtaaaac gcatacctct atttctcttg gtgtaagctt
     5821 taattccgtc aatcgtgcag ttagtctgct ggtatgagta acatcagagc gataaaggtc
     5881 gtctaaccac tctgcagaat gaagtatttt tgttaataaa ggggtcagtg tgaatattat
     5941 caaaataatg ccaactgaaa ttagggcaat agcagataac aatgctgcag aattaacatc
     6001 ttttatagca cttattattg aagaacctaa aatgtaaaat atagcaactg aaaaaatacc
     6061 gactctaaaa aatataatac ttcgagattt ttttgcaata atacccatca tataatatat
     6121 actaacaaaa cccattgaat aagaaactcc aaacaataat acctgcagta gaatccattc
     6181 ttcaccattg cttccaaaag cgtttataac aaaacccatt gtaagaatag caaatgaaaa
     6241 attaagtata tagcatatat tacagtgtaa aatcaggtga aggaaaaggg taataacaat
     6301 tcccagcaaa acaccaatca catgataagt atttaaagtt aaggacgcta ttgatgtaat
     6361 tgatctccac aaggcaggag ccagaaagtc gttaaataga aaaacaaagc aggcaagtat
     6421 taacacagta taatctctta gttctgtctt tttcccgggt tcaaaacgtt catcctctgt
     6481 tgttttgcca taaagccatg cgcaaatcaa aattggcaaa aatccaacta atggtaatac
     6541 attaaaaatt ccattttcat aattcgtatc agcaaaaatg actttagcaa caagcataat
     6601 tttagatata aatataccta tactaactga ataaagcctt tccaggttat tcataaccat
     6661 aaaaaatccg taatctattg atgctaaaat gtttcccata gcaatagctg ttattgttgt
     6721 accaatatac aaaattacag tattacttgt tatagtgtta atcaaaatcc ctattattcc
     6781 aacaatggca ctatacttta caaatggcaa aaactttttt aaatcaagca gaaagacact
     6841 ggcagtagtg gctaaaggat acaaaaaagt gaaaattgtc attggtgaat atgctgatag
     6901 tttaccaacc tcaccatatg tcccaatagg tagaatataa aaatccagcc aaagaattat
     6961 agcaatccag ctaacaaaaa aaggcaagtt cttttttgtt agcttattta tgtttagaac
     7021 tccttcctgt ctaaatgtac cgaggaagga atttattgtc ttctgaaaaa ttgctttcac
     7081 ccaatgtccc cccttatttg ttttgcatct gcaaacaaaa gttgcagatt gaatatcttc
     7141 tttattgtta gtataaaatg taaagacctc atatttataa tatatgcttc caaaatttgt
     7201 accattaaat atagtatatc taagtttgca gaagtttcaa ctcaatgtta atacgtgggt
     7261 gcatatttta aaagtgaaga aatatttacc aggagctgta aagaaagaag tatcatcttt
     7321 atgtgaacgc aaatcattga atgcgtactt caagagcatt ttttgaagca aagtatagtc
     7381 gtaagtaaaa tctagcacat catctttttc atatttccga acaaaataat caaaaattat
     7441 ctcttttgcg tacgaatcga tatatgaaat tctattgttg ttgcaatttg cgcacacatc
     7501 ttttaccatt ggatctccca agtggacttt gtctctaata ctatcaattg tggcaaagca
     7561 ctactctggg aaaagatcta atatggcgca agaaataata tgctcttttg ttccttttgc
     7621 ttcctgccca caataagcac aaatcactat ggcttcactt cctatcttta taaactcacc
     7681 agcagagtca tcgcgtaaaa gctggttgtt aggcttctta ttattgctat tatataccat
     7741 cagttaaaaa ccagtttcct atacagataa tggaaaggtt acttttagcg aacgtacctg
     7801 cttatagaat ttctgtgcat gaattcatta taaatatacc cttgttccgg cgttattcct
     7861 ataagctctg gacgctcttt atatatttct tccaacatct tatctaagcc tccgataata
     7921 ctgaaaggtt cttcagcttc aataggataa tcagaaatta gttttttaac caattcttcc
     7981 gcatgaccct ggttcttggc aaatacaata ccttcaaact tgctggcagc ttttctgctg
     8041 tttcttttat gataataaaa atgtactgaa taaatcatat ggttctcctc tcattaccct
     8101 attgcttata aacggttact acctgctgac tccacgatat agttatagct ctggcccttg
     8161 atttatataa aatcacatac cacttttttc acaaacatct tgtgagctta ttcaatggta
     8221 taaacttaca tacccgcttt aattatccaa agataaatct cttttataat taatttaatc
     8281 aacctgctct gaaaaaagct gagttcttaa ctctccatct tcaaaccaaa ctagccaaca
     8341 tttatcattt cgactgcgca gtttaggata tttctcaagt gcttttgaag tagcttgtct
     8401 aagtaatcta acagtccaaa aaatcttgaa attcagatgt tctaattgca aaagcagttt
     8461 ttctcgatca acacataaat atgattgtga gtcatgccac ttttctcctg cttcaaacct
     8521 aattgcttca agctgttttt ctccgttata gtacttatac ccatctccat cacatatgtc
     8581 cagtaattcc cgtataattc gggaaggtag tttataatat acatcaccat attcaggata
     8641 attagctgtg cactcttcaa ctgtcttatc aattgtatat ttcacaaaat cgccatcatc
     8701 aatagaaata ttatagattt tattgtaaca ctcatttttc caattcatcc agcatatctc
     8761 tttaggggtt atataacatt gagtctcagt agatgaatgt aaatcctcag gattactcag
     8821 gttataaact aaagagtctt ttcgatttat taagtcattt tttaagtatt taaattgctc
     8881 atttgtgatt attgccgaag aaatccacat aagtgattct ccacccaaag cattagatac
     8941 agagttaaaa ctatataacg atatccaatt ctttttctct tcttgtacaa tttcatggtt
     9001 gaaatcacta atatcaatcc atttttcaaa attaggagta ggagcattta ttatccaatt
     9061 ccttatggca tcttccccca tatctttgtt aattaaaggt gatagttcct ctgggataaa
     9121 ccaagaggtt ttttccataa tgctatctgg attttcttga tataattctt gcgctggatt
     9181 tggaaaatca tcaagcaaac cataatcgct cagcaatgat ggtacccttt caaaatctct
     9241 aaatggtaac cgatctgata aatagcctaa aatttcattg cgagcacacc atgtatactt
     9301 ctcgcaaaaa gtcattatac tacttttgga accgtgcgtt gcaggatgat gttggcgcaa
     9361 aattgcaata tcaacaccga ttaattcccc aggtttacct ccattaggac aaccgtagaa
     9421 ctcttcttca ttccatccct gtagtagaat gtaagcatat gcagctgata ggacaaattg
     9481 attaggttca agtgattcca tttccaaaat tcgcgcatgt tctaacaaga agctgtctgc
     9541 ttctttattg tatttgtaag caggcgattt cgaatttgga atggtggtat ctgcaccatc
     9601 atcaatcaaa tcatccagat gatcccaaaa tttcctttgc tttttgtatg caagctgaat
     9661 ctctctcaat ttttctctac atttctcatt aagaaaaatt tcttcgttag tcaatatatc
     9721 ttcaatttct ttttcagaaa aataccatgt atagtcaatc tcttcagcaa ttttatcact
     9781 accgcggcgg ctgaaaaaca ttctatccat aggatcacac agaacatatc ttgccaaatc
     9841 ataatcaatt ggtccaaagc cacccatccg ggtaccagaa gtagcctcac gattaagagg
     9901 gatagttcca cttgtatgat atggtggacg gctctcacct acatcctcat catttatggt
     9961 accaaatgca taggaccgtt caacaatagc tctgctgtaa tatcgaatag cacaattata
    10021 atgcatacta attttatctg aagcaaaaat attttttatc atccaatcac tgaataattt
    10081 aatgcatgga tgttcttttt caagcataaa tacagtcccc attgctatgg aaaacaaatc
    10141 ttctttcatc tgaggatcgt tagtgttcca tgtatactcg aacagcttat aaaattcaaa
    10201 aggttgcgta aaagcccatt tcattagttc tcgtctataa cccactcttt caatattatt
    10261 aacagtagtt agcatccatg catagactaa gggaatacca ttatatttgt cctcgctaca
    10321 taatttatat cttttatcgc ttaaatcaat ttttgtataa catttccaag gagcatctcc
    10381 tgggtacctt aaatagctag gtacagacca tataatgtca cgttttcccg cagtttcaaa
    10441 acctataaga tactcatgta atatctgtgc accaagagaa tgattctcaa ttcgtgatac
    10501 cggaagaaca attttattca caacactttt taaacagtcc gaattataat tcatcatcct
    10561 tttaaccaaa ggtatgtatt tagatgcagg gtttggtgat acgtttgata atgaaaagca
    10621 tactaaatca aataattcgt tattacttaa attatctctg aagcttttat tctcagttat
    10681 caaatagctg taatcttcca aaagaaggat agagaccatt tgcagagcac cctcatgacc
    10741 tgttaatgtt tctgtaagtg taacttttga tggattttgt atttgatcag taattaatag
    10801 tgcaagtata taatcaaaaa aaggttgaat gccaatcgaa taataggttt ttggtggctc
    10861 aagtatagta tctgaatttg ctgtatagct ctgaagtatt ccataatctt caataaaatc
    10921 aataaattct cttcggtaat catgggcgac ggtaataagc cctggaacat ctttcagaga
    10981 atcaattaaa tcatttctat acaccgttgt atttattaga aactttcttg ctactgctaa
    11041 taatgaattt tgcacaataa tctctctatc tccataccct gtttcatatt tggagttaaa
    11101 ttctctttca attatatgaa atttcttcga taaaagatta gtaatagtaa cagaagattt
    11161 ttctacgccg gatatacttc tatgcctata attttcacaa aaaatacgca atgcaagggg
    11221 agtttttaag ctccatttaa tccaagaaca cccttcgata tttatacgaa aatattcaat
    11281 atattcttga aataattcgt ttactggaac atcaccatca gaaggtagtc ttaatgtatt
    11341 actcaataag ctatcatcat atgccaattc tctaaataca tatggtcgac ttgtaataca
    11401 aaaccttagc cggggatatt tgtaacatat tgcttctatc tctcgaaggc gatcatacca
    11461 caactcatat ggtgtagatt catccacccc ttcaatgcaa attaatatct tcggaatgat
    11521 tttagctaca atagtatctg ctgtagagac attattaatt tctgcacgat atgacaatgc
    11581 ttctaaagcc tgccataatt ctgtttcgtt ccaaactgaa gctaacccta aagacttttt
    11641 taagatatca atccagctat tatttggatt aacatctttc gcgcggatta atatcggaat
    11701 atgtaatttt tcgtttagta atgattccac catgttagct atcccatgtg tttttcccga
    11761 accaggattg cctaatatca gatatttttt attatctaat ttaaaacgaa tatccgaaat
    11821 aatagtatgg atttcatttt cctgaagtgc ttcggcagtt tttttaatct cattaatgtg
    11881 aaagtaattt cctctaaatt gctcgttttg ctttaacaac tctaatagca tagcaaagtc
    11941 aatataccaa ttattttcgt taatcgagga tgcaacttgt tggtcattat ttacagcatc
    12001 tatagtacat tttatctcac ctaataattt aattacttgc tcttgagttc cctgaatttc
    12061 atgtattaac tgctcctgtg atgaatgatc ttgaacaaat tgtaaatagt catcgcatgc
    12121 tttcaaaaat ttgctgcatt gctggctcat agcactcagt gtacttatac tattttgacg
    12181 atcatctaat gttcctacaa attttaaaat gcttttttgc atttgtcctt gaatatgcag
    12241 acaaggagta tacttctgcg ataaccatcc acttttttgt ttttcgtaag agtacacaat
    12301 aagctctttg gaaatctcgg atttttcaaa ccaatatcgg tatatccctg cagcatcact
    12361 atcttgcaat ttagttaaaa gttgagtttc attccataac tcaattttca accccggata
    12421 atcatcctct aatctatttt tcaactgctg ccaacggttt gattcagtat tatctataac
    12481 cttttttcct ttaccaatac gatccgatga taaatcacgc ggaatacata caatatactg
    12541 ctttatcgat ggtcttactc taagtgcagt tttaatcgag ttgctaattt gcccgaattg
    12601 atttgcagaa atactattca agaaccactt tgcttgcatc ccaataattg tatcttcttt
    12661 ttttatagca aaggattcaa ctcccccatc tcctccagag ccatttacaa ttgtaactgc
    12721 ttttaaagag tcaggatatg tttttttgca ccacaattca aacaactgat tgcacatgca
    12781 ttcaaaagct cgtgctggtg cctcattata agtttggaaa tgttgccagt tcatattctc
    12841 acccctttta tctaatatgc catttttctc attagccata tttcatgact gtctgccagc
    12901 ttaaaatctg acttattatt agcaaagtat tgcacccagg ataatctatt atgaccttcg
    12961 acaagctgat atggaagctt ataattccct tcattgaatt ctgaagattt cataacaata
    13021 atagggattt tccatgtccc atatgtatac caatgattga tcagttcgtg caaatgacca
    13081 taagaacttc tgttaatatc tccatttatt tttttcaagc tttctattct ctcttgtaga
    13141 tagagattgc taaacttggg aggctttatt ttcataatat tatcaactga aagtttcatt
    13201 aattcgaatt tgacttcata ataattaatg catgaaaatt catcaacaaa ccctgaaaaa
    13261 ttgtatagct tataaaaact ttgctcaagc acactttcag ggaattttcc tacttctcta
    13321 ttaatcctct tgcaatattt gagaaatcca tctgtattcc tccaatcgct ttcatactca
    13381 tttggagtaa agtgtttcca actatcatgt tgattcataa ggttttctcc aggatctatt
    13441 tttcagttta tgtatttatc gacaacaggg tataatgctt ccatagcatt aataaactga
    13501 ccccattccg gattcttttg tttttcatgt gacgtaaggt aatactttat atagcattct
    13561 caaagcttta ttatcacttt tatctatttt ataaagatag aaaatcagtt catacatttt
    13621 tatcacttgt acattgtcca aacctgtcat ccattcttcc gctttttcta ttaactgaac
    13681 agtgttggaa tcatgggtag caaaataaat caatcctttt acagctatgt atgcaagaga
    13741 ttttacttca ccccggtcgt ctttattatc aagcaaagga tcatattttg ttaaaggata
    13801 aattttttct tctattgaat ttctaagcat atattcttct gccgtcagtg atgaatcctt
    13861 atgaatagta agacgcggag gtgaattgct gatgtttgtg tcaatgtaag tcctgactga
    13921 tggtagtacc agctcatcat atacagcttc atggatagcc aggttaggga atgcttcaaa
    13981 tattggctca agccatatgc gttgaaatac gagaaaatcg aaggattttt ttgcatacct
    14041 gcttctatcc ggtggaatta gaaaattggc atctaaagta atgacttgag ccggattact
    14101 aaaaacactt tctaaggtcg ggtttgggga ggagagattt atatccatca ctaaccctcc
    14161 tcgttaaaga attcttcaaa ttcatctata tcttcatcag acacttcgaa cttataacca
    14221 aagtcgtcag gttttttgtt gaagaggttt aatgtattta tgaatttgtc ttcatctata
    14281 atgccatctt caaagttgcg aataataatt tcgatatcct ttggagaagt accaatatta
    14341 tttgtagccg tgttaagctt tacaaatact tctttattaa tagccttacc catacgacca
    14401 tattcaccgt ccaaatcacg ctcgtcaatg ctatacagtc gttcatattg ttcatcagaa
    14461 acagcatcaa tttctcttaa tctcttgaca agcgaacgat atgggagcca ccaaacacat
    14521 tgcaatctaa ctataaagcg aagtaacatt tttgtttgta ttttcctaag tgaagatgtt
    14581 ttaaattcat caagcacaat actttttaat gtgctttcag gaaggaggaa ttcagcagca
    14641 aatcgattag cctcagcttc tacataacta tcctcttcct ctgacaatcg actaagatgt
    14701 gaaccggtct ttgtgtaaaa atgatatagc tcatgggcaa tggcaaatat ctgcttgtca
    14761 aaaaagtcag cagtatttaa accaatgaaa acaagctctt tatccccttc cgtggagcac
    14821 atgagtgccg cagagaatgc cggttttaca tcatctaact taataggata ttctaacaat
    14881 agaatattca tattttcaag aattgtaaaa atgtcattag caattggtgt ttcacctata
    14941 aagcccagtt tgcgacgctc atcccctgct aacttacgaa tattttcaat ttgaatttca
    15001 ttaagagaga ttgagtgatt cattttcaaa tttactcctt aacaatattt gctgcctaag
    15061 agatagcatc atggtaaaaa gtttttctac cccttctttt tcagattcac ttaatgtttc
    15121 actgcgcaaa gcaataaatg agttcgattc ttgtttttca atgaacatat caacaatatc
    15181 aaactcaagc gtattggcca aagactccaa ctggataata gaaggaataa agttctcacg
    15241 ttctatgcgg cctatcatta ctctatttat accggtaaga tcacttagct gttcttgtgt
    15301 cagtcctttt tcctcacgtt ttaacttcac tatttctgca agttttgcaa cagataattt
    15361 tttcattttt caagcctcct tttaatacat agtattccca ctcttaacta tatgatacta
    15421 taaatttcat ttttggtcaa caaataagta aaatatgagc tttgatttgt ataatgaaat
    15481 ttaggattgc atcattttaa acaaagacct attcatttaa accacgcaaa gtgtaaaata
    15541 gaatattttt attttcctcc cgctatcact tttatttcag cgctaacatg tcataaataa
    15601 actttcggct tattctcgct cccgcggctg ctctattctt aatacaattc tcagatagtc
    15661 gttataatcc tttccacgct tcgtcggctc atcgtaaaca gcatatctgg acgggagaat
    15721 ggtttgaatg gctttcgtcg ccaatcgccc tggtatgtcg ttatccaaat gcaaaacaat
    15781 atcttcaatg tacggaaaat cctttaagta ttgcatgagt ggggcaggcg gagtactctc
    15841 ctcaatattc tttttcggcc tgtaaattcc cgcaagggat agacagtttt ccaacttcca
    15901 gtccctgcca gaaagcagtt tcagtgtgcc ataggacaat aaatcaatag cgctttcaaa
    15961 cagatgcagc ctgcagcatt gcttcgggga gtgtcatgtc cctcactttg atgagatagt
    16021 ccagggcaga acgtccaccg atgtcgcgtg accaccagtt ccattttcta ttgctaattt
    16081 tcagactgtc atgacttcgt gtagtataga cattgcctga aaagcgtacc agctcataag
    16141 gctcgtaata ttgcagataa gtaagcagat ccatttgctt tgctcgttgg atttgttctg
    16201 gtgttacata gggcatagtt tatcacctct aaaaatgaaa cagcatcggg gtatttaccg
    16261 atgctgtttc atagattgtt ttgttgtaag ttcataatat accgattttt ttcaagtagg
    16321 ccatacagtc cgcatgtcct tgaaaataaa tttccatgtg ctccatagaa ttcgcctgtt
    16381 cattcactct taaatattca accagtgcct tatgttcgtc attgttcaat gatacgccgc
    16441 cctcgccatc cacagctttt tgtacggctg gatattgccg ctgtatttct attcgcctgt
    16501 aataaagctt gctatattcg tcgttgctgt gaagcaggtt cataacaata tcattgtcga
    16561 tttcaccgaa taattcttcc actcgtttga ctaaattgtt caggataaaa tcctgagaat
    16621 cctttgaatt gtcgagtaga ctcattcttt tctcctttct tgtgagttag gcgaaaatta
    16681 ag
//
LOCUS       DAOSDD010000085        16529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1417_length_16529_cov_5.155214, whole genome shotgun sequence.
ACCESSION   DAOSDD010000085 DAOSDD010000000
VERSION     DAOSDD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_16529_cov_5.155214"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..8889
                     /locus_tag="PK733_09130"
     CDS             373..8889
                     /locus_tag="PK733_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10740.1"
                     /translation="MSKKVIALILVLSLVLSAAPFSYASEIADIPSDTFRVSDEYAAQ
                     YPNGVIEFESVGFDTTEGDEDFDVIVLRRGGTSGRVEVDFKVIEITAKYGDDFSIFVP
                     KWYGYNILKKETDSPTLLESSLDKNKDGFITSAGDETATDKQPATEEESIDEDETAAE
                     EQPVAEQEAVPQEELVQLEAAAPVYKSSLHKLRDEALGEISEAPELINTPMNIFDIQD
                     PEQIKIGDALNTLMPGVAGHLVFEDGENYKTFKVRINDDSIFEGPEQFIIGLFNPTDG
                     AVLGDAYNATFNIIDNDVGAGQIIGFEQDYYYVYPSEKKAEVIITRTGDIHTYAKIEI
                     STLSGSAKADEHYAPVMTDAMFLPGETNKRVLIPLLADSVAEMLSFEIVTTQDEKPTS
                     GTNRATISIMPEEEKNGMLLMSDWETNSISFMPNQEASRISLMSAQNDFSLQTIRDDG
                     SYIYISGNEFTHENSFYYNLQGYKYSGPQPANWGSEGKQMSLEYSRYAPYGYSSIYKW
                     VDLRGISGITLKWRNTGTNYYNVSTVQINDTNYYQLNGSFGGDAGGDTYISASTNKYP
                     RIQAPLRLVYNTSNCSGSLGIWSMKLHKQQFKVRVEDAIPLTYYTWDGETKRESEPFN
                     PGTVIPGNWNPYRDETITLTPELSDEGKLRGVEFVGYQIKRAQKSEGIIGTPLGGKIG
                     YSSIIESKAITLTPEFIAGNIYGNGSSEYEEELIIRPVFKRVETSLKIANYDDTKGDL
                     LIGGKKYKNEALNTEGFLSGDVLITSLVPASFYSPAGFTQYTVNNYGQIVPKEYKFPS
                     DIILTDKKLNPIVEPIFIEDNKTISIEWKSLGIGTAEPKGINNLRGMILHDHPLNMPQ
                     NERNILYNGVPYMSPPKFENYNDFESYKAGMAYYRSILTQKEAKIAQNYNTYRQQYDK
                     FTLERLHIGDIVTLYARPDKDYTVSWMIDEGQSGPVLDKKVTRKLVHIGSSFSFEVTD
                     KIKKVYYSFVNVNQPGNAIFEGKVVKSKNTIKDQREYAIDPEDPSTFTGVPGVTVSIA
                     VAESKNHSVTVFGQEYETTALTDSEGDFAIYAPYAVNGFLYSVKLTNGDIVYPKSVSV
                     SDYMYLEILFMDNFKVMDMDIKLDKIAQDKITIEDKQAVISLKTYSEDNRRISKVRLR
                     SYNSLGYLWQTMDAEIVGNNTWQVTANLRESFKDGGRLTVELYDDRDIGHGEIESGYM
                     ISDLPEPKDVVLPVLQSTGPGYNLQPIGEIKPETDLGRTRSLSPVQEGSNSKTYAIAV
                     GAGEVLKQVVADNPRKFDNQKVAQKAKILASYLNDGRYSSNLVNKSWDKSKGASKTGP
                     GKGGLTVDFDVGFYIQLTKATDGNITDYYFDYAMIYVASKVQAKQDINASICGVPVYL
                     TIKGSGMVRGLMVAEGGNDTRVLTTYGYFPVGENYEGIDKFGLFHINLKFAIGTGIGF
                     RGPAAIGVEGEMYLDVASQPPCEGKGTLTFALNAEIDFLMIPIKFNVAEYTVELFKSD
                     GYIENDWLPEVTKRNTMQMLNNMVNEQGVSNISVLDRASMSGWNTVPDGENAGIREIE
                     TTTLQTGIYKHPQPKLISLDGNKKILFFINDDLSREGYDRTALYYSIYDGQWNPPVML
                     QDDGKIDYDPYARIVGDKLLVTWSGSNRKFGETQPSVADLLNSTDIYAQFFSLDGEKI
                     GETAQLTFDNGTYGNMSPKALYDEKTGSIMVIYNKTDYKTEGVTFDGDALKAGDYLHN
                     SYSTIAYRMYVNGEWQTTYNSDETTYLDYEQKYGAGSLYGQRFLDFGIGGLTPKVTEI
                     AASSRDGRALIMYTLDIDNNPHTESDTEIFMKIYDFTDRSITPPIRITNNLVEDSNPQ
                     AVEYNGHSYLFWNNDGNISYLDINALIDLNLMEETIDGVLYYTLNKDYESFSTVLNEK
                     NAEAAESFTVTIGDEGNLYVVWSELGQTVFNTGEQETVIKERQLYATMYDAQFTGIFD
                     VEDGELVYMGGWGTKQKLTETSGEYNNEQTVAVDSDGMVTIVNRRFEIVDDADAPGSK
                     KESDTSALVARRFEPKSTLYINSSDIKVYPEYPGAEDNVVLTISAVNEGFMPSDSVTF
                     KFEMYDDEHGWVQYCDNIVAHSHISSGSNVTADTIFTVPDDFSEENPVKLRITVWEDD
                     FEDSATVEHYTIWPKENLVIENQDGYIAAKNKVRITGLFLNTGSKDAENVKVTIEKIV
                     DEAMTIVDESMIEEDSEIPDPEIIKTFTYDNVKTSNVYDIDELIEVSDADFGKDERIE
                     FSINIKKVINNEETTIYSKPVIVYKNPQMDTEISDIIINNNLPINIKKGTSKNADARI
                     QPYGAASAYKLRYTSLNPEIAEIDASCGIISALKKGTAEILVEAIRVNSVLLSDTNNQ
                     IYTSAGSFVDYNDDGSIKGYIEADRSDIVMATKSVFINVTDPEPDPDKEPDPDKEPDP
                     GKDPEPDEEPAPDEKPDTNKDTEPDEKTGSDKESENSTNGDSTDASDVIEIIIEDDRV
                     TLYIKGDSTLGQSDVAEALSKLGNKIQQINNLVINEGDKLNIDAAALKTLSDNELTAE
                     IILKNGGILLSKDVLEEIAAKADSNVVISINKTESSDGRPIVDIEITSGSEKITGFGG
                     KEITIKIPYTLKDDEDENAIVVYYIDENREQRMVTNGEYKDGFVIFKTNHLSKFAVGY
                     NKVEFMDVSGWAKDYITYLAARRIVNGIGSNKFAPENNVTRAEFVKMLAVLSGDTLSE
                     NVETQFSDVAKTEWFAPYVAWAAKNGYVLGTSKTTFAPKDSITREQIAVIIERFLKAK
                     NYKFQSQGVPKAFTDEADISTYAKDAVMTLSNINIISGKGSNIFAPKENATRAESAKM
                     LAALISIMLE"
     gene            9183..9842
                     /locus_tag="PK733_09135"
     CDS             9183..9842
                     /locus_tag="PK733_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HOJ10741.1"
                     /translation="MNTYKPNEFAEMIGVSVKTLQRWDNDGKLKAFRNPSNRRYYTHN
                     QYVEYMGKIVQDKDKRKTVIYTRVSTNGQKDDLKNQSEFLKQYANEKGIIVDEVFEDV
                     GSGLNYNRKKWNKLLEDCMLGLIKTIIVSHKDRFIRFGFEWFERFLKSNGVELIVVNN
                     ESLSPQEEMIQDLISIIHVFSCKIYGLRKYKTKIKEDEEIVKSLQNRDKPHQPAKTKN
                     S"
     gene            9772..10947
                     /locus_tag="PK733_09140"
     CDS             9772..10947
                     /locus_tag="PK733_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ10742.1"
                     /translation="MKRLLKAYKTEINPTNQQKQKIHKTIGVCRFVYNFYITHNKEVY
                     EKEKRFVSGMDFSKWLNNDFIPNNPGYKWIKDVGSKAVKQSIMNAEQAFKRFFKGQSK
                     IPCFKKKKNQDVKAYFPKNNKTDWAVERHRIKIPTLGFVQLKEKGYIPTNGKVKSGTF
                     SYKAGRYYVSVLVEEDIKILYNNTNDGLGIDLGIKDLAVVSNIEKPFKNINKTKKVKK
                     LEKKLKREQRKLSRKYESLKIRNKKEGGNATRQNIQKQVVTVQKLHNALTSIRDNYIN
                     QVVNEIVKTKPSYITIEDLNVSGMMKNKHLARAIAQQKFYQFKTKLQSKCVLNGIELR
                     IVDRFYPSSKMCSKCGAIDKTLNLSDRTYICKECGCVIDRDKNASINLANAKTYKIA"
     gene            11148..12644
                     /locus_tag="PK733_09145"
     CDS             11148..12644
                     /locus_tag="PK733_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ10743.1"
                     /translation="MNIKKKTVLAMIAAVILIAAAVFIFFIVSKKSDAQKSDFSKLSW
                     TDAFSKLHGRISKEYAFTEWKGIEWQKLYEEYLEKIEAAQLKNDFEVYYTNLRKYLHE
                     VPDGHVGMNNIREIDDKYIGGGFGLAAAKLDDGRIIITWVDESGPAWAAGIRTGAELI
                     EWDGQPVTDAVVAVSTIFTSIPATEENLESKRLQYLVRAPISKQISIKFLNQKNSELQ
                     LVSITAYDDNKKSLTKNYPDSVVSDKLRNMIVGVEDPNPMPEAMVEKKTLNGNISYIK
                     IWGEFDADLQETGKAQSTLELLRLAVQESIEEKSTGIILDLRNNLGGLDDMAADILGS
                     FYSEKTFYEYQNVYNPATGERKIQPANPKTGSLALYIEPAKQCFNGRIIALINTKCVS
                     SGEGIAMGIRNLPNGETLGFFGTNGSFGLSGAEAVMPGGLIVRWPSGQSLDENKNIQL
                     DSRDGIGGVSPSIPIPMSFENAIRVSNGEDIELEEAIRILGVCSKILG"
     gene            12841..13119
                     /locus_tag="PK733_09150"
     CDS             12841..13119
                     /locus_tag="PK733_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10744.1"
                     /translation="MADCKAFGTPLSDEQLDDMGGGTDSGNDCGVPESQFPCIEKCCS
                     KSISMSGTVYKKNCPYCSIWSSFPDSTSLEVTYILDCILYGYGKRMKV"
     gene            complement(13232..14290)
                     /locus_tag="PK733_09155"
     CDS             complement(13232..14290)
                     /locus_tag="PK733_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOJ10745.1"
                     /translation="MKIFDYLEKYDHEQLVFCHDRETGLKAIIAIHDTTLGPALGGTR
                     IWNYESEEDAIIDALRLSRGMTYKNAAAGLNLGGGKTVIIGDASKIKSEELFRVFGRY
                     VEGLSGRYITAEDMNTTTQDMAYVNDETDYVVGLEGKSGDPSPVTAFGVFRGIQSAVD
                     EVYGSDDLTGKAVAVQGLGAVGYNVCKHLHEAGAKLYVTDIRKESIERVVNEMDAIAV
                     APDEIYKVDCDIFVPCAMGAVINDFTVDQLKCKIVAGSANNQLAEEKHGDTLMEKGIL
                     YIPDFVINSGGVINVYEEMKGYNKERAMARASTIYDSIKKLIEISKRENISTNKAAER
                     MAEERIEKIGRAKTIYLK"
     gene            14702..15733
                     /locus_tag="PK733_09160"
     CDS             14702..15733
                     /locus_tag="PK733_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HOJ10746.1"
                     /translation="MGDKIILRKAILHILDTNIDIPVLSNTELELDSDICDFLEKHIE
                     KLLDDPNMKIAEFLCSANEMGETNYIRDICANLAKDAEYFYQASFDITNILFNILIKN
                     VDIAPADLICCLFDYCRQSYLGILKLNYKTSFTHFVQQCEEGNINTIIKHRTILPSES
                     QKIDECAFVNLQDLSIKLIEKAYEINGEKELYFSRLFLCCSPELSDNEKLKIIDKVTK
                     KINKKFFDEDFDKVAKLKKVVSENLEESNSIQVDTIIEKVFDNNIEIQNEYVSEIQKE
                     GLLEKSISVPEKLAEKKFKTHKIKTDTGIEISFPPSYYSDNEKIEFINNPDGTISIII
                     KNIGKIVNR"
     gene            complement(15798..16187)
                     /locus_tag="PK733_09165"
     CDS             complement(15798..16187)
                     /locus_tag="PK733_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10747.1"
                     /translation="MRANFFCYAKGEGNSQNVNNQGQKCIAKLTVLQPVFQYVENNLD
                     RPMKIDELSAIVNMSEKYFISYFKSALGITPGNYITQLKMNKARDYLYQEKYSIKEIA
                     NLLGYSDQYTFSKAFKKYFNVSPSKFI"
BASE COUNT         5801 a         2648 c         3362 g         4718 t
ORIGIN      
        1 acaggtaccc catacttttc tgccttttgt tttatgtact cttgcagctg ataaatggtc
       61 tattgtaaaa aaatatagaa aattttgttt ttgttagtga ttttgttagt aattgcgtga
      121 tataatgaaa ctaaaaattt cataaaaata acatatttaa atgtgcacta tatactatta
      181 ttctcaaaaa gcaaacaaaa aggaaaactt ttttgcgtaa aaccatgtgg tgcaggcgta
      241 acatgagact ttttgagaat atatagttcg ttaatttatc gtagtgcacg ctcatttgtt
      301 tgtagctata cgctgagata taaatataac aaaatgggaa aaaggtaaaa tgttaatttc
      361 tgggggtgtt ttatgtctaa aaaagtaatt gcattgattc tagtattatc tcttgtttta
      421 tctgctgctc cattctcata tgctagtgaa attgcagaca ttccaagcga tactttccgg
      481 gtttctgatg aatatgcagc gcaatatccc aacggtgtga ttgaatttga atcagtgggg
      541 tttgatacaa cagaaggtga cgaagatttt gatgttatcg ttttaaggcg tggcggcact
      601 tctggcaggg tagaggttga ttttaaggtt attgaaatta cggctaagta cggagatgat
      661 ttttctatct tcgtgcctaa gtggtatggt tacaatattc tgaaaaagga aacagattca
      721 ccaaccctgt tggaatcatc attagataaa aataaagatg gttttattac ttcagctgga
      781 gatgagaccg caactgataa acaacctgcc actgaggagg aatccataga tgaagatgaa
      841 actgctgctg aggaacaacc ggtggcagaa caagaagctg taccacagga agaactggtg
      901 cagttggagg cagcagctcc cgtttacaaa tcatcactgc ataaattgcg tgatgaggct
      961 ttgggagaaa taagcgaagc ccctgagctg attaatacgc caatgaatat atttgatata
     1021 caggatcctg aacaaataaa gattggtgac gcattaaaca cacttatgcc gggagtagcc
     1081 ggtcatctgg tttttgaaga cggggaaaat tataagacat tcaaggtaag aattaatgat
     1141 gacagcatat ttgaaggacc ggagcaattc attatcggac tttttaatcc gacagatggt
     1201 gctgttttag gggatgctta taatgcaacc tttaacataa ttgataatga tgtaggtgca
     1261 ggacaaatca tcgggtttga acaagattat tactatgtat atccttccga aaaaaaagcg
     1321 gaggtcatta ttacacgtac cggtgatatt catacatatg caaaaataga aatatcaact
     1381 ttatccggaa gcgcaaaggc ggatgagcat tatgctcccg ttatgaccga tgccatgttt
     1441 ttgcccggtg aaacaaacaa aagggtttta ataccgcttc ttgctgacag tgtagctgaa
     1501 atgttaagct ttgaaatagt gactacccaa gatgaaaaac caacaagtgg aacaaatcgg
     1561 gctaccataa gcataatgcc ggaggaagaa aaaaacggaa tgttgcttat gtctgactgg
     1621 gaaacaaaca gtatttcatt tatgcccaat caggaagcaa gcagaatttc gcttatgtct
     1681 gctcaaaatg attttagtct gcaaacaatc agggacgacg gatcgtatat ttatataagc
     1741 ggcaatgaat ttacacatga aaattcgttt tactataatt tgcaaggtta caaatatagt
     1801 ggaccccagc ctgcaaattg gggatctgaa ggaaagcaaa tgtctttaga gtacagtcgt
     1861 tatgctccgt atggttattc ttccatatat aaatgggttg acttaagagg tattagtggt
     1921 ataactctca aatggcggaa cacaggaact aattattaca acgtatcaac agtccaaata
     1981 aacgatacca actattacca gttaaacgga tcttttggag gcgatgccgg cggtgatact
     2041 tatataagtg cttctactaa taaatatcca agaatacagg caccactccg tcttgtatat
     2101 aatacatcaa attgttccgg aagtcttgga atttggagta tgaaattgca taagcagcag
     2161 ttcaaggtca gggtagaaga tgcaataccg cttacttact atacatggga tggagagaca
     2221 aaaagagaat ccgaaccttt taatccgggt acagtaatac ctggtaattg gaacccgtat
     2281 cgggatgaaa caataacact aaccccggaa cttagcgatg aagggaaatt acggggagtt
     2341 gaatttgtcg gctatcagat taagagagcc caaaaatcag aaggtattat agggacgcct
     2401 ttggggggaa agataggtta cagcagcatt attgagtcaa aagcaataac actgacaccg
     2461 gaatttattg caggcaacat atatggaaac ggtagcagtg aatatgaaga agaactgata
     2521 atacgacctg tttttaaaag ggtggaaaca tctttgaaaa tagctaatta cgatgataca
     2581 aaaggcgact tgttaatagg tgggaaaaaa tataaaaatg aagcattaaa tacggaaggc
     2641 tttttatcag gtgatgtcct tataacatcg ttggtacctg catcttttta ttcacctgca
     2701 ggctttaccc agtacactgt taataactat ggtcaaattg taccaaaaga atacaagttt
     2761 ccttcagaca tcatattgac cgataagaag ctgaatccca tcgtagaacc aatttttatc
     2821 gaagacaaca agacaatatc cattgaatgg aaatcattgg gaataggtac tgctgaacca
     2881 aagggaataa acaatttaag aggcatgatt ttgcacgatc atcctcttaa tatgccgcaa
     2941 aatgagagga atatcctata taatggtgta ccatatatgt caccacccaa atttgaaaat
     3001 tataatgatt ttgaatcata taaggcaggt atggcgtatt accgcagcat acttacccaa
     3061 aaagaagcga aaatcgcgca aaactataac acctaccgtc agcagtacga taaatttaca
     3121 ctggaaaggc tgcatattgg tgatatcgta accctctatg caagacctga taaggattat
     3181 acagtctcct ggatgattga tgaaggccaa agcggtcccg tgcttgacaa gaaagtaaca
     3241 cgaaagcttg tccatattgg gagcagcttt agctttgagg tgacagataa aataaaaaaa
     3301 gtatattatt cttttgttaa tgtaaaccag cccggtaatg ccatatttga aggtaaggtg
     3361 gttaagtcta aaaatacgat aaaagatcaa agggaatatg ccattgaccc ggaggatccg
     3421 tctactttta caggtgtgcc gggggtaaca gtatccattg cagtagcaga aagtaaaaac
     3481 cattcagtaa cggtatttgg acaagagtat gaaacaacgg cattaactga tagtgaaggg
     3541 gattttgcca tatatgcgcc ttatgcagta aacggctttc tctattctgt caagctgaca
     3601 aacggtgata tagtttatcc caaatctgtt tcggtaagcg attatatgta tttggaaata
     3661 ctgttcatgg ataattttaa agtaatggac atggatataa aactagacaa aatagcacag
     3721 gataagataa ccattgaaga caagcaagct gttatatctt taaaaacata ttccgaagat
     3781 aatcgcagga taagcaaggt gcgcttgaga agctataact ctttaggata tttatggcag
     3841 accatggatg cggagatagt tggcaataat acctggcaag ttacagcaaa tctccgtgaa
     3901 agcttcaaag acggaggaag attgaccgtt gaattgtatg atgacaggga cataggacat
     3961 ggtgaaatag aatcagggta tatgattagc gatttgcctg aacccaaaga tgttgtacta
     4021 cctgttttac agagtaccgg acccggctat aatctgcagc caataggcga gataaaacct
     4081 gaaaccgacc tgggcagaac tcgtagttta tcacccgtgc aagaaggttc gaattctaaa
     4141 acctatgcta ttgcagtagg agcgggggaa gtattaaagc aggtggtagc cgacaatccc
     4201 agaaaatttg acaatcaaaa ggttgctcaa aaggctaaga ttttagcaag ttatctcaat
     4261 gatggaaggt actcttcgaa ccttgtgaat aaaagctggg ataaatccaa gggagcatca
     4321 aaaacaggtc cgggtaaggg cggtcttact gttgactttg atgtgggatt ttatattcag
     4381 ttaaccaagg caacggatgg gaatataact gattattatt ttgactatgc aatgatttat
     4441 gttgcaagca aagttcaagc caaacaagat attaatgcaa gtatatgcgg tgttcccgta
     4501 tacttaacta taaagggaag cggaatggta cggggattaa tggtagctga aggtggtaac
     4561 gatacaaggg ttttaacaac ctatggatat tttcccgtag gtgaaaatta cgaaggtata
     4621 gataaattcg gccttttcca cattaattta aaatttgcca ttggaacagg aattggtttt
     4681 agagggcctg cggctattgg ggttgaaggg gagatgtacc ttgacgtggc ttcccaacca
     4741 ccctgtgaag ggaaaggaac ccttactttt gcccttaatg cggaaattga ctttttgatg
     4801 atacccataa aattcaatgt tgcagaatac acagtagaat tatttaaaag tgacggttat
     4861 atcgaaaatg actggctgcc tgaggtaacc aaaagaaata ccatgcaaat gctaaacaat
     4921 atggtcaatg agcaaggcgt gtcaaatata tccgtgcttg accgggcaag catgtccggt
     4981 tggaatacag ttccggatgg cgaaaatgca gggataaggg aaatagaaac cacaacgctg
     5041 caaaccggta tctacaagca tcctcagccg aagttaattt ctctggacgg taataagaaa
     5101 atactgtttt ttataaacga tgatttgagc agggaaggct atgacagaac tgccctgtat
     5161 tattcgattt atgacggaca atggaaccct ccggttatgc ttcaagacga cggaaagatt
     5221 gattatgacc cctatgctag gatagtaggg gataagctgc tggttacctg gtccggttca
     5281 aacaggaagt tcggtgaaac gcaaccctct gtggccgatt tactgaacag caccgacatc
     5341 tatgcacaat tcttcagttt ggacggtgaa aaaatcgggg aaacagcgca attaaccttt
     5401 gataacggaa cctatgggaa tatgtcgcca aaggcgcttt atgacgaaaa aacgggaagt
     5461 atcatggtta tttacaataa gacggattat aagaccgagg gtgttacatt tgacggcgat
     5521 gcattaaaag caggtgatta cctgcataat tcatacagca caatcgctta caggatgtat
     5581 gtaaacggag agtggcagac cacttataac agcgacgaaa ctacatacct tgattatgag
     5641 caaaagtacg gggccggttc actttacgga cagagatttt tagacttcgg tataggcggc
     5701 cttactccga aagtaaccga aatagcagct tcaagccggg acggcagggc attgatcatg
     5761 tatacccttg atattgataa taatccgcat acagaaagcg atactgaaat atttatgaaa
     5821 atatatgatt ttaccgaccg gagcattaca cctcccatca ggattacaaa caatttagtt
     5881 gaggattcca atccgcaggc agtagaatac aacggacatt cctatttatt ctggaataac
     5941 gacggtaata tttcttatct agacattaat gcactaatag atttaaacct tatggaggaa
     6001 acaatagacg gcgtattgta ttatacattg aacaaagact atgaaagctt ttcaacagtg
     6061 ctgaatgaga aaaatgccga ggctgcggaa agctttaccg taacaatagg agatgagggc
     6121 aatctttacg tggtatggag cgagttaggg caaactgtat ttaataccgg agaacaggaa
     6181 acagtcataa aagaaaggca gttgtacgca actatgtatg atgcacagtt taccggtatt
     6241 tttgatgtcg aagatggaga actcgtttac atgggaggct ggggtactaa acaaaagctg
     6301 accgagactt ccggggaata taataatgaa cagactgttg ctgtggattc ggatggcatg
     6361 gttaccattg tcaaccgcag atttgaaatt gttgacgatg ccgatgctcc agggagcaaa
     6421 aaggaatcgg atacatccgc actggttgca agaaggtttg agccaaaatc cacactttat
     6481 ataaattcat ctgatataaa ggtatacccg gaatatccag gagctgaaga taatgtagtg
     6541 cttaccatca gtgctgtgaa tgaaggcttt atgccatcgg atagtgtaac atttaaattt
     6601 gaaatgtatg atgatgagca tggctgggtg caatactgtg ataacatagt cgcccattcc
     6661 catatttcat ctggcagtaa tgttacggca gatacaatct ttacggttcc agatgatttt
     6721 tcagaagaaa accctgtaaa attaagaata acagtttggg aagatgattt tgaagatagt
     6781 gccaccgtag agcactatac catatggcct aaagaaaacc ttgtcataga aaatcaggac
     6841 ggttatattg ctgcaaagaa taaagtaagg atcactggct tgtttttaaa tacaggaagt
     6901 aaagatgctg aaaatgttaa ggttactatt gaaaaaattg ttgatgaagc aatgactatt
     6961 gttgatgaat caatgatcga agaagattct gaaataccag accctgaaat tattaaaaca
     7021 tttacatatg ataatgtaaa aacttcaaat gtatatgata ttgatgagtt aatcgaagta
     7081 tctgatgctg attttggaaa agatgagcgc atagagtttt ccattaatat taagaaggtt
     7141 attaataatg aggaaacaac gatatattca aagcctgtca ttgtatacaa aaacccgcaa
     7201 atggatactg aaatttcgga tattataatc aataataacc ttcctatcaa tataaaaaaa
     7261 ggaacctcga aaaatgcaga tgccaggatt caaccctacg gagccgcaag tgcatataaa
     7321 ttgcgatata caagcttgaa tcccgaaata gcggaaattg atgcatcatg cggtattatc
     7381 agcgcattga agaaaggaac tgcagaaatt cttgttgagg ccataagagt aaacagtgta
     7441 ttattatctg atacaaataa ccaaatttat acctcagcag gaagctttgt agattataac
     7501 gatgacggtt caataaaagg ttatattgag gctgatcgaa gcgatatagt gatggcaact
     7561 aaaagtgttt ttattaatgt tacggatccg gaacctgatc cggataaaga acctgatccg
     7621 gataaagagc ctgatccggg caaagatccc gagccagacg aagaaccggc tccagatgaa
     7681 aaacctgata cgaataaaga tactgaaccg gatgaaaaaa ccggttcgga taaagaatct
     7741 gaaaattcca ccaatggtga ttcaaccgat gcatctgatg tgatagaaat cataattgaa
     7801 gatgaccggg tcacacttta tattaaaggg gattcaaccc ttggacaaag tgatgtagct
     7861 gaagccttaa gcaaacttgg caataaaata caacagataa acaatctcgt aataaatgaa
     7921 ggtgacaaac tgaatataga tgcagcagca cttaagactc tgtcagataa tgaattaact
     7981 gctgaaatta tactcaaaaa cggtgggata ttgttatcca aagatgtact ggaagaaatt
     8041 gctgctaaag ctgattccaa tgtagttatt agtatcaata agactgaaag tagtgacgga
     8101 agaccaattg ttgatattga aattacatcg ggcagtgaaa agataaccgg cttcggcgga
     8161 aaggaaataa caataaaaat accatacaca cttaaagatg atgaggatga aaatgcaatt
     8221 gtggtttatt atattgacga aaatagagag caaaggatgg ttacaaatgg tgaatacaaa
     8281 gatggcttcg taatttttaa gaccaatcat ctttcaaaat ttgcagttgg ttataacaag
     8341 gtagagttta tggatgtttc aggctgggcg aaggattata ttacctatct tgctgcaaga
     8401 agaattgtaa atgggattgg cagcaataaa tttgcaccgg aaaataatgt gaccagggct
     8461 gaatttgtaa aaatgcttgc tgttttgtca ggagacactc tatcagaaaa tgttgagact
     8521 caatttagcg atgttgccaa aactgaatgg tttgcacctt atgtggcatg ggcagcaaaa
     8581 aatggttatg ttcttggtac atctaaaaca acattcgcac caaaagacag tataacccgt
     8641 gagcagatag cagtgattat tgaaagattc ttaaaagcta aaaattataa attccagagt
     8701 cagggcgtcc caaaagcatt tacagatgag gcggatataa gcacttatgc aaaagatgca
     8761 gtaatgacgt taagcaatat aaatatcata tccggtaaag gtagcaatat ctttgcaccc
     8821 aaagagaatg ccacaagagc ggaaagcgca aaaatgcttg ctgcactaat atctataatg
     8881 ctggagtgaa ctcgttcagc taaagctgaa catcggggaa tcagatggag actctactcc
     8941 acctgattta ggtcccacct atagaggtgg gagtcttaac ttaataaggt gtaatgataa
     9001 atttttgcta aacgtagtta agcttagccc aatataaaaa attaagtcct gataaataaa
     9061 aacaagccat ctgcttggat ttaagattcg atattgatat cgaagaggag ctaacaactg
     9121 taataaaata tgttgacaaa catagacgta cgtggtagta tatagacagg aggtgtttat
     9181 atatgaacac atataaacca aatgaatttg cagaaatgat aggtgtgtca gttaaaacat
     9241 tgcaaagatg ggataatgat ggtaaattaa aggcttttag aaatccttct aatagaagat
     9301 attatactca taatcagtat gttgaatata tgggtaaaat tgttcaagac aaagacaaga
     9361 gaaaaactgt tatttataca agagtttcaa caaatggaca aaaagatgat ttaaaaaatc
     9421 agtctgagtt tttaaaacaa tatgctaatg aaaaaggtat aatagttgat gaagtatttg
     9481 aagacgttgg tagtggttta aattacaatc gtaaaaaatg gaataaacta ctagaagatt
     9541 gcatgttagg gttaataaag acaattatcg tttctcacaa agacagattt ataagatttg
     9601 gatttgaatg gtttgaaaga tttttaaaat caaacggagt agaattaata gttgttaata
     9661 atgaaagttt gtcgccacaa gaagaaatga tacaggattt aatttcaatt attcatgttt
     9721 tctcttgtaa aatatatggt ttaagaaaat ataaaactaa gataaaggag gatgaagaga
     9781 ttgttaaaag cctacaaaac cgagataaac cccaccaacc agcaaaaaca aaaaattcat
     9841 aaaactattg gagtttgtcg atttgtttac aatttttaca ttactcataa taaggaagtt
     9901 tatgaaaaag aaaaaaggtt tgtttcagga atggattttt ctaaatggtt aaacaatgat
     9961 tttattccta ataatcctgg atacaaatgg attaaagatg ttggaagtaa agcagtaaag
    10021 caatccatta tgaatgctga acaggctttt aaaagatttt ttaaagggca gagtaaaatc
    10081 ccctgtttta agaagaaaaa gaatcaagat gtaaaagcct atttccccaa aaataataaa
    10141 actgattggg ctgtagaaag acatagaata aaaattccaa cactaggctt tgttcaatta
    10201 aaagaaaagg ggtatatccc taccaatggc aaagtaaaaa gtggtacatt ttcatacaaa
    10261 gcagggagat attatgtttc agtattagtg gaagaagata ttaagatatt atacaataat
    10321 actaatgatg gattaggaat tgatttagga attaaagatt tagcagttgt aagtaatata
    10381 gaaaaaccct ttaagaatat taataaaact aaaaaagtaa aaaagttgga aaagaaacta
    10441 aaacgtgagc aaagaaaact ttcgaggaaa tacgaaagtt taaaaataag aaataaaaaa
    10501 gaaggaggaa atgctactag gcaaaatatc caaaaacaag tagttacagt acaaaaactt
    10561 cataatgcac ttacaagtat aagagataac tatatcaatc aagtggttaa tgagatagta
    10621 aaaaccaaac catcttatat aactatagaa gatttaaatg taagtggaat gatgaaaaat
    10681 aagcatttgg caagagcaat agcacaacaa aagttctatc aatttaaaac taaacttcaa
    10741 agcaaatgcg ttttaaacgg gatagaatta cgaattgtag ataggtttta cccatcaagt
    10801 aagatgtgca gtaaatgtgg agcaatagat aagactctca atctatcaga tagaacatat
    10861 atatgcaaag aatgtggctg cgtaattgat agagataaaa atgcatcgat aaatcttgca
    10921 aatgcaaaaa cgtataaaat agcataataa agctaatatg cgtatgtacc gatggctagt
    10981 cgggagttta agcctttgga gtgttaaacc aaatgttagt agggtagtaa tatccgagaa
    11041 cagacacgat gaataaggaa atttctcaat atgggtatgt ttgtccatat tttgagtagc
    11101 agatgatttc actgaataac atgaacataa cagaaaggat gtccacaatg aacattaaga
    11161 aaaaaacagt acttgcgatg attgcagcgg taattctgat tgcggcagca gtatttatct
    11221 ttttcattgt ttcaaaaaaa tctgatgcac agaaaagtga ttttagtaag ctttcgtgga
    11281 cggatgcttt tagtaaatta catggaagaa tttctaaaga atatgctttt acggaatgga
    11341 agggaattga atggcagaaa ctatatgaag aatacctgga gaaaatagag gctgctcaat
    11401 tgaaaaatga ctttgaggtg tattatacga atcttcgcaa atatctgcat gaggttccgg
    11461 acgggcatgt aggaatgaac aacatcaggg aaatcgatga taaatatatt ggcggaggat
    11521 ttggtttagc tgctgcaaaa cttgatgatg gcagaattat cattacatgg gtcgatgaat
    11581 ctggtcctgc atgggcagcg ggaatacgta caggtgctga actcatagaa tgggatggcc
    11641 agccggtaac cgatgcagtg gtagctgttt caactatatt tacaagcatt ccggctacag
    11701 aagagaattt agaaagtaaa aggctgcaat atcttgtccg tgccccgatt agcaagcaga
    11761 tcagtattaa attcctaaac cagaagaact ccgaactgca actagtttca atcacagcct
    11821 atgatgataa caaaaaatca ctgacaaaaa actatcctga ttcagttgtt tcagataagc
    11881 taaggaatat gatcgtgggt gtggaggatc ctaatcccat gcccgaggca atggtagaga
    11941 agaaaacttt aaatggaaac atcagttata ttaaaatatg gggtgaattt gatgcggatt
    12001 tacaggaaac gggaaaagca caatcaaccc tggaactatt gcgtctggct gtacaagaat
    12061 caatcgaaga aaaatccaca gggataattc ttgaccttcg gaacaattta ggcggattag
    12121 atgatatggc tgctgatatt ctgggttcgt tttattctga aaaaacattt tatgaatacc
    12181 agaatgtcta taatccagcc accggagagc gtaaaatcca gccggccaat cccaaaaccg
    12241 gttcacttgc tttgtatatt gaacctgcga agcagtgctt taacggccgg attattgctt
    12301 taataaacac aaaatgtgtc agctccggag aaggaatagc tatggggatt cggaatcttc
    12361 caaacggtga aaccttaggt ttctttggaa caaatgggtc gttcggtttg tctggtgctg
    12421 aagccgtaat gccgggtggg ctcatagtcc gttggccatc cggccaatcc cttgacgaga
    12481 acaaaaatat ccaattggat agccgggatg gaattggagg agtaagcccg tctataccga
    12541 tacctatgtc gtttgagaac gctatacgtg tttcgaatgg tgaagacata gagttggagg
    12601 aagcaattcg aattctagga gtctgctcga aaatactggg ttaacaggtt ggactgcaag
    12661 aggaacggtc atggactgcg ttatgataca agaagttgca ggttcccagg tatactttct
    12721 gaagataatt agcatttgat acctatgagt tgctcttcaa aataaactac gggtactttt
    12781 tgggaaaata tgagaaaaat ccatttttgt taacgatttt tgggtggagg tggaattcag
    12841 gtggcagatt gcaaagcatt tggcacaccc ttaagtgatg aacagcttga tgatatggga
    12901 ggtggtaccg attcgggtaa tgattgcggt gtgccggaga gtcagttccc ctgcattgaa
    12961 aaatgctgca gcaaaagtat ttctatgtct ggtactgttt acaaaaagaa ctgcccatat
    13021 tgcagcatat ggtcatcttt tcctgatagt acaagtcttg aagtgacata tatacttgat
    13081 tgtattttgt atggttatgg caaaaggatg aaagtataaa gtatagtgat acaagaaaat
    13141 aagacagaca aaatgctaag actgccactc ctgccggaaa aggtggtctt agcattttaa
    13201 ttaatatcga ttaatatctt agaaaagatt attattttaa atagattgtt ttagctctgc
    13261 ctatcttttc tattctttcc tcagccattc tttcagctgc cttattggta gatatattct
    13321 ctctcttgga tatttcaatt aatttcttta tactatcata aatagtagag gctctagcca
    13381 ttgctctttc tttattatag cctttcattt cctcgtacac attaattacc ccgccgctgt
    13441 ttattacaaa gtctgggata tagaggatcc ctttttccat taacgtatct ccatgttttt
    13501 cctctgccaa ttggttattt gcagagccgg caacaatttt acattttaat tggtcaactg
    13561 taaagtcatt tattactgcg cccattgcac atggaacaaa tatgtcgcaa tcaaccttat
    13621 atatttcatc tggagcaact gctatggcat ccatttcatt tacaactctt tcaatgcttt
    13681 cttttctaat atctgtgaca tacaattttg ctccagcttc atgtaaatgt ttacatacat
    13741 tgtatcctac tgcacctaat ccttgaactg caactgcctt tcctgttaaa tcatcactac
    13801 cataaacctc atcaactgca gattgtattc ccctaaatac tccaaaagct gttactggag
    13861 atggatctcc gcttttacct tccaagccta ctacataatc agtttcatca tttacatacg
    13921 ccatatcttg ggtagtagta ttcatatcct ctgcagttat atacctacca cttaaacctt
    13981 ctacatatct tccaaatact ctgaataatt cttcactttt aattttcgat gcatctccaa
    14041 taattacagt ctttccaccc ccaaggttta atcctgctgc tgcattttta tacgtcatcc
    14101 cccttgataa tctcaatgca tcaatgattg catcttcttc tgattcataa ttccaaattc
    14161 ttgtgcctcc caaggctggt cctaaagtag tgtcatgtat agcaataata gcctttagtc
    14221 cagtttcgcg atcatgacaa aaaactaatt gttcatggtc atatttctct aagtaatcaa
    14281 atattttcat aaataaccct ccaactattt agtatgttat tataatacta atatgccaat
    14341 ttggaaagcg atgtcttccg ggataatttt tgatagcatc gggaagatgt cgcggggaaa
    14401 atgtctctat agaaggcctt gcagcccttc cttcagatgc attccttacg ggtcagtaaa
    14461 aattaccctc tggaaatgtg ctttccattt acaatataac aaagtagtat taataatatt
    14521 ttttatattg tataattata aacctgaata gaaaggtttg caagatttgg ttttaaatat
    14581 gagtcattgg tgtgaacata tttgcatgtt tgcagttaaa taaattttgc atttaaatta
    14641 aaaataattt ataataattt ataatatttt aatatttaca aaataatgaa aggctaaaaa
    14701 aatgggtgat aagataatat taagaaaagc aatactgcat attttagata ccaatattga
    14761 tattcctgta ctttccaaca ctgaacttga actggatagt gatatatgtg actttcttga
    14821 aaaacatatt gagaagctgc tagatgaccc caatatgaaa attgctgagt ttctttgtag
    14881 tgctaatgaa atgggtgaaa ctaattatat aagagatatt tgcgcaaatt tagccaaaga
    14941 tgcagagtat ttttatcaag caagttttga tataacgaat attttattta acattttaat
    15001 taagaatgtt gatattgcac ctgcagattt aatatgctgc ttatttgatt attgccggca
    15061 atcttatttg ggtatattga agcttaacta caaaactagt tttacccact ttgtccaaca
    15121 atgtgaagaa ggaaatataa atactatcat aaagcataga actatattgc cttcagaatc
    15181 acaaaaaatt gatgaatgtg ctttcgtgaa cctgcaggat ttgtccatta agcttataga
    15241 gaaagcatat gaaattaatg gtgagaaaga attgtatttc tcaaggcttt tcttatgttg
    15301 ttcgcctgaa ctctccgata atgagaagtt aaaaataata gataaagtta ctaaaaaaat
    15361 aaataaaaag tttttcgacg aagatttcga taaagtagct aaattgaaga aagttgtatc
    15421 agaaaatttg gaagaaagca actccattca agttgataca ataattgaga aagtttttga
    15481 taataatatt gaaattcaaa acgagtatgt ttctgaaatc cagaaagaag gtttactgga
    15541 aaaatctata tcggtacccg aaaagcttgc agaaaaaaag ttcaaaactc ataaaataaa
    15601 aacagatacg ggaatagaaa taagctttcc tccaagctac tatagcgaca atgagaaaat
    15661 tgaatttatc aataaccctg atgggacaat ttctatcatt ataaaaaaca ttgggaagat
    15721 agttaacaga tgatgagaag gtgttatggt agaaaaaggt gttgtatatg cagatcaagt
    15781 attcatatca agtattgcta gataaatttt gatggcgata cattaaaata cttcttaaac
    15841 gcttttgaaa aagtatattg atcagaataa cctagaagat tggcaatttc ttttattgag
    15901 tatttctcct ggtaaaggta atctcttgct ttattcattt taagctgggt gatataattg
    15961 ccaggagtaa tacccagagc gcttttaaaa taagatatga aatatttttc cgacatattt
    16021 acgatagccg aaagctcatc aattttcatt ggcctatcca aattattttc tacatactga
    16081 aaaactggct gaaggactgt tagctttgct atgcattttt gcccttgatt gtttacattt
    16141 tgactattcc cttccccttt tgcatagcaa aaaaaattcg cacgcacata gatcccgccg
    16201 gaggtatata attcacgctg tgtactgtaa gacatcatca ggttactata ggacatttta
    16261 aaaatatatc taagccttat aatgaaaatt aagtgaatac agtgtatata agtaaaaaac
    16321 gtgatgcttg aaaaatatct atttaaaaca tcaaaattca tcaaaattaa taataaacaa
    16381 cgaaattaaa aattattaat aagcaaggag tgataaaaac atgaaaatcg aaaagatcca
    16441 acatcccaaa gtagtcgtta ttggtgcagg aagcttgttt tttggaagac aggctatatg
    16501 gcaaatggtg acttcacctt atctaaatc
//
LOCUS       DAOSDD010000086        16468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1425_length_16468_cov_5.299945, whole genome shotgun sequence.
ACCESSION   DAOSDD010000086 DAOSDD010000000
VERSION     DAOSDD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16468
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_16468_cov_5.299945"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK733_09170"
     CDS             <1..480
                     /locus_tag="PK733_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10748.1"
                     /translation="VSVVGSTYCKFAEDDTAPDSDNAKFGDSKAGGIFDVEDLAMGFV
                     RFDNEACLSLEFSWASNIEEECRYVELLGTAQGAKWNSSDPILKIFGEANGEIIDIFP
                     KISSKGKRMGHAGNIDHFVDCLVNKAEPVFVPEQGVDMMKILDAFYRSAKEKREIIL"
     gene            498..1505
                     /locus_tag="PK733_09175"
     CDS             498..1505
                     /locus_tag="PK733_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ10749.1"
                     /translation="MSFLANNPFKTKYSLEFRKDETFKVIQFADLHWESLGEELDDID
                     QKTLGIMEKLIGIEEPDLIVLTGDIMTSGRKSPVRLSKTVDDVKKAVSIILKPVLESG
                     IPFAFTMGNHEHCHPVDSFTLIKALSEFPSSLVQSGNRKSGGAGDSVIKIKGSKDKKD
                     KWIIYFLDSGCKYEYPVKTYGWIEREQINWYVKQSRRITQKNRRTVPALAFFHIPLPE
                     YMEVWNRKKCYGVKGEEVSCPVLNTGLFSAMVQNKDIKGVFVGHDHDNDYNGNLYGVE
                     LCYGRTTGYRMHQKDSFALGARVILLNERENGFNTWIRLENGMVLKKDIDDIQQITLS
                     N"
     gene            1611..2357
                     /locus_tag="PK733_09180"
     CDS             1611..2357
                     /locus_tag="PK733_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ10750.1"
                     /translation="MIINEPIYDLNCLAQYNLHIHSTYSGCAKPEMTAPNVLECAKNN
                     HIKIIAFTDHYNSKDTKIINNNENLKKQAEACNTNIKVLYGSELSAYGIGKYLDDIDI
                     NQQLGYRLYSYNHYHLEYWEHPEEKTPRGYVNHALALLEHLLKSKRADCIAHPFIGRF
                     IRCFDDRTLVTKEIKDNELGDILELGKDNEVAWELNVNSIIADPEFGRRYWNIGKEVG
                     VVFNMGTDAHRLQYISTLQYLEKIKEILGE"
     gene            2478..3194
                     /locus_tag="PK733_09185"
     CDS             2478..3194
                     /locus_tag="PK733_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ10751.1"
                     /translation="MQVMRNLFQVGGSLNGITHATKEDASYEDCNTYILKTDKGLIMF
                     DCGCGSTLEQIYKNMEYWSLDPSDIKFCFITHPHLDHAGAAYLLSKQQIKLYAHKNTV
                     DAIAAGDERCCGYLYHKKFIPCTLDYGLSDGDIVNILGVNIKAMYMPGHTMGCTAYLF
                     QHEGKLIVVSGDIIGTLLDGYFGWNGSIDFNKQTYIETLRQFAKLDSDVMLPGHGMIY
                     FHKPRRRVEEALNMALIQWR"
     gene            3382..5043
                     /locus_tag="PK733_09190"
     CDS             3382..5043
                     /locus_tag="PK733_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10752.1"
                     /translation="MRRKIFCIVLAISIFLTVFAAACSQKTTDAGNRNDSGTSSATTG
                     GSDNKNETTEGYTLPIVKEPITLVFLKRENSTPGSSFMNNRPLVWEEYEKKTGIKVKF
                     EALPSNELKEVIQLRLAAKQNIPDVIEIPGTQDGTYLEKYYADGVIIALNNYIDKYAT
                     NIKSIFEKYPDYKKSLTLSDGSITGLGALRATKYEFQATGIRVDILKKLNLQMPKTPD
                     ELFNVAKAFKAYDINGNGKADEFGITGNGDQWKELGDAWGLHYVTGDGWTVRDGKVVY
                     EPVTPEYQDFLRFLRKCVKEGIFPADWNTVDSKTNDARIINGQTGIMARMNALSSVYY
                     LDPNGTMLKNFPDAEWSVIPPLDGPYGKGILTKEPVAQRWRTFVVTSANKYPGETVKW
                     MDYAAFSEEGQTYNRFGVENMNYRIENGKIIRFTKEEQEKAMPEGAWSGSGYLPSVDT
                     DESTEMGFAHIVALDHPKVQELRDLWKYIEEPFVPPIPSVNDGKKLASLLTDVKTQVD
                     EMFFKFLNGDANIDTEWDNYVKRLNANGLDDVISIYQKGYDSLNK"
     gene            5156..6109
                     /locus_tag="PK733_09195"
     CDS             5156..6109
                     /locus_tag="PK733_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10753.1"
                     /translation="MKIEAINRASITTSIKQSPFIRNFKNEIFMHALVLPTVVYFFIF
                     AYMPMYGILVAFKDFSFAKGIWGSNWVGLEHFMTFFKSMYFPRLVKNTVLISVYSIIW
                     GMPFPIIFALLLNEIKNRHFKRIIQTVSYFPHFVSTVIIVGLMVSFMSPVDGIVNILR
                     VRYGHEAINFIQSTKWFRTLYIGSGIWQGFGWGSIIYLAALSGISNELYEAAYVDGAT
                     RWQQTIHISIPGIMPTFIILFILSVGGIMSVGSSKIILMYTPATYDVADVISTYVYRK
                     SLIGGEFSFGAAVGLFNTVINYALVFVTNKISKKVSDISLW"
     gene            6124..7026
                     /locus_tag="PK733_09200"
     CDS             6124..7026
                     /locus_tag="PK733_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10754.1"
                     /translation="MAMKMSMGEKVFEKFNYIILTILAFLTVYPFIYIISNSVSSLDA
                     IMKQEVYLLPKGINIDSYKLVFKHGGILTAYYNTIWYTVVGTAISVFLTTSSAYPLSR
                     KTYSIRSQLMVYFAITMYFGGGLVPVFILVNQLGLYNTRWVMVILGAVGVSNMVIARV
                     FFQTNVPEEMTDAARIDGANDVIIYFKIALPLSKAVVAVLTLQYAVSHWNDFFKAMIY
                     LNETHLQPLALYLRRLLIMGTAVEFAGEQEERLREMDQFALNAVSQQFKYASVVITML
                     PIMCFYPFLQKYFVKGIMVGAIKE"
     gene            7067..7819
                     /locus_tag="PK733_09205"
     CDS             7067..7819
                     /locus_tag="PK733_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ10755.1"
                     /translation="MSQQQQKLLLESKKYKAVFFDFGDTLAFNNETFPESLYKIVKSI
                     GIGVDINCLRSVIKKADYGELAEERMKCRDRDSYRNFRIKYYRYVLKMIGYSHMEKHA
                     EYIHNIINFYNRTYLKPESLYVLDTLRSDRYKLGVVSNFSHALPWYCDELGLTEKLDF
                     ITYSDDIGHEKPESHIFNDALDKACVKPDEVIHVGDSYKADVLGAKQIGITPVLVLPN
                     YDKEYEDCLCINNLIDILNIMGIKHKYSVDFI"
     gene            7883..9223
                     /locus_tag="PK733_09210"
     CDS             7883..9223
                     /locus_tag="PK733_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ10756.1"
                     /translation="MEYKNDKLNIVQIIWHDLGRHLSCYQEGSVSSPNLDRMAREGIL
                     FTNHYSTGPVCVPSRCSILTGRYPHAQELWRFSNKEVTLPKTLKEAGYSTYRFGFHEE
                     KEFNTLNKDIDIELMGKELLGYEYTWKESRKPMDIADRFCEFLGNQLTDKPFFANVAF
                     YHVHRPNIEEVDEETVNNTKLPAILPDLPDTYESKEDVAILEKKIKTADEAVGKILDC
                     ICSQGLKDKTLVYFTTDHGIDLPRAKMTVYDAGIQAALIFWGPDFIQAGKVDNNLHSH
                     MDIFPTLMELINNPIPKRVQGSSFAPVFTGTDYTPREFVIVERTWEASDDPVRAIRTP
                     KYKYIRNYCPGWPIPVPPDYTRKVGKEIIEKIYSNPRPFEELYDIENDPCELNNLAND
                     ENHKEVKDEMWRTLENILIEDSDPVLSISEYMSYLKELVPGRWVKEGKRFTFNI"
     gene            9239..10999
                     /locus_tag="PK733_09215"
     CDS             9239..10999
                     /locus_tag="PK733_09215"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ10757.1"
                     /translation="MYIVKNRNSAMKIVISENASISVRHAARELQHFLRQMTGVTMPI
                     KTDNMGVTGSEIYVGYSKRTEQAGIFPHAELGGEGYVIKHVNGNLIIAGNDPRGTLYG
                     VYSFLEEYCGCRWYTSDFSYIPQCKNIELDDNLDITYSPVFEYRETFYYDSFDGDFAV
                     RNHLNGMHMRLKDEHGDKIKYAKGYFVHTFSRLVPPEKYYDDHPEYFALRNGVREREN
                     AQLCLTNPDVLKISIEKTLDELRKNPDVNIISISQNDGTQPCTCPQCQAVVEEEGSEA
                     GPILRYVNKIAEEVEKEFPNVAIDTLAYDYSRNPLKITKPRSNVIIRLCSFECCFSHP
                     LDDCRQTADLKKRSKKKSDDFAGDVEKWSKISNKLYIWDYVTNFEFYQLFHPNLHILA
                     PNIRFFARNNVKGVFEQGNGQGEYGEMAELRTWLLAKLLWDPDFDVEKGMNEFLAAWF
                     GPAAGAMREYINLMKEALLESGYHISLYDAPKAPHITVELLSKADEIFDRAEKLTSQM
                     PIENRRVRKERVGIKYARFFNEEPGTDREAKVEEFMNYTKDLRIKVFSEKWGSENIRR
                     LAIRGVWPPNKEDSIVIRDK"
     gene            11052..14411
                     /locus_tag="PK733_09220"
     CDS             11052..14411
                     /locus_tag="PK733_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ10758.1"
                     /translation="MSIMRKKILLNKINQLKNCIYASFENLDNWETRIGVFKAAGNNE
                     ITNEQWENIAIGTRWEAKDDIVRWFRRNITIPSDFQGKKVILDIEVGGEGLVYINGEM
                     KSAITSYIEPWAATRTRVLLTPAAKGNESLDVLIEAGLNYMEFADYRRKGMESVQYEF
                     RSAKLRQIDTKVEKYYFDAIVTYEVIEVLEGKGSERLPRNIKEMITSLVGFDKYGTLI
                     HAKLVDALESSLLTLDFDFSRERLLASIDEAECILNEKLNEIPHFPQGQVILTGHSHI
                     DTAWLWPIKETIKKCSRTFSNTIALMDEFPEHNFAQSQPQLYEYTKKYYPELYKKIRE
                     KVKGGKWELIGNSWVECDTNVPSGESLVRQILYGRNFFKDEFGKWSDIFWMPDVFGYS
                     WALPQIIKRSGMEFFYTAKLNNNDVNRFPYSLFWWQGIDGTRVISYLQRATYNGEINP
                     SFINELWTNYDEKELHSEVLGTYGFGDGGGGPTYEMLEYSKRLMNFPGMPATKIDSAY
                     SFFENVKLHSCNLPVWNDEMYYEFHRGTYTSQAHTKRNNRKSELLYRQSEIASSIALN
                     KFNNEYSIEQLTEGWKLILLNQFHDIIPGSSINEVYRDCAVDYKKIFAIGSDIEDKSL
                     QAIISNVNFDYMNENTEKISIENAGIIEEEALYSYIVVFNYLSWPVTGPVEAVISINN
                     SKGYTFESLALEDIKGDKIPCTFKKIDEQTVAVSFEALDVPSMGYTSYKLVKVDEEMQ
                     VGKTIDNSTFDHIKRITVTKELMENQFFKINVDNNGTICSIFDKKAGREVLSEGGHGN
                     VLQIFEDRPARESAWNIDIEYQKKSWVINEVKFVEVKEVSETKGVLRVVKAFNKSEIV
                     QDITIYASTPRIDFYTHVNWQETEKLLKAAFEVEILSPCATYEIAFGSIRRPTHWNTS
                     WDQAKFEVPAHKWADLSEGGYGVSILNDCKYGYDIKDNEMRITLLKAPVYPDPVADKG
                     THEFVYSLYPHIGDWINGNVVNAGYELNVPLKAICINKDKNDQKSASQGVKNAKASLP
                     PVASFVQIDKVNVIIDTLKAAEDGDGLIMRLYESSGRRGKVKISTELDITNVTECNLM
                     EEDEGRIDFKNGELCIDIKPYEIKTLRLRKS"
     gene            complement(14705..16285)
                     /locus_tag="PK733_09225"
     CDS             complement(14705..16285)
                     /locus_tag="PK733_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOJ10759.1"
                     /translation="MPITEMLSRNARMYSDEVSLIEREPAIGSRKEITWLEFENQSNK
                     IANCLIEHGITRGDKVILLLMNCLEWLPSYFGILKAGAMAVPLNFRFTAEEIKKCIEA
                     AEGKVLIYGPEFCERVDAIRNSLLSVESYIFVGEKRKTEETCYMDITYSELMGQFPSD
                     NPGIEISDYDEAALYFTSGTTGMPKPIILTHANLVSACITENRHHLQSHEDNFLCIPP
                     LYHTGAKMHWFGSLIVGSKAVILKGVKPQWILEAVSEEKATIVWLLVPWAQDILDAIE
                     SGEVDLKNYNIKQWRLMHIGAQPVPPSLIQRWKKYFPDHLYDTNYGLSESTGPGCVHL
                     GIENIHKVGAIGLPGFNWEIKIVDCEGNPVTNGTVGELIIRGPGVMKGYYKNPEATAK
                     VLRNGWLYTGDMAKKDEDGFIYLVDRKKDVVISGGENIFPVEIENYLQAHEDIKDAAV
                     IGMPDRRLGEIPVAVIETKPGRQLTESMVIEFCRDLPKYKRPRKIIFDKVPRNPTGKI
                     EKPKLREKWCGNTKTVFV"
BASE COUNT         5576 a         2526 c         3430 g         4936 t
ORIGIN      
        1 gtttcagtgg ttggctctac ttattgcaag tttgctgagg atgatactgc acctgattct
       61 gataatgcta aatttgggga tagcaaagct ggaggaatct ttgatgttga agatctagct
      121 atgggttttg tacggtttga taatgaagca tgtctgtccc tagagtttag ctgggcatct
      181 aacattgaag aagagtgcag gtatgttgag ttattgggaa cagctcaagg tgctaaatgg
      241 aattcgtcag atcccatatt gaagatattc ggtgaagcca atggggagat tattgatata
      301 tttcccaaga taagcagcaa gggtaaaaga atgggccatg cagggaatat tgatcatttt
      361 gtagactgtc ttgttaataa ggcagagcct gtctttgtgc cagaacaggg agtggatatg
      421 atgaaaatac tggatgcctt ttaccgttct gctaaagaaa aacgggagat cattttataa
      481 atttatgaga ggtggctatg agttttttag ctaataatcc ttttaaaact aaatattctc
      541 ttgaattccg caaggatgaa acatttaaag ttatccaatt tgctgatctt cactgggagt
      601 ctttaggaga agaattggat gatattgacc aaaaaaccct tgggattatg gaaaaactta
      661 ttggcattga agaaccggat ttaatagtat taaccggtga catcatgaca agcggacgta
      721 aaagtcctgt aaggctttca aagactgtgg atgatgttaa gaaagctgtt agtattattt
      781 tgaagccggt attggaatca gggataccat ttgcttttac catggggaat catgaacact
      841 gtcatcctgt agatagtttt actttgataa aggctttaag tgaatttcca tccagcttag
      901 tgcaatcggg caaccgaaaa agtggagggg ctggagatag tgttataaaa atcaagggat
      961 caaaagataa aaaggataaa tggataattt attttttgga ttcaggatgt aaatacgaat
     1021 atccggtaaa gacttatggc tggatagaaa gagaacaaat aaattggtat gtaaagcagt
     1081 ccaggagaat aacacaaaaa aatcgaagaa cagttccagc ccttgcgttt tttcatattc
     1141 ccctgcccga atacatggag gtatggaata gaaaaaaatg ttatggtgta aagggtgaag
     1201 aggtttcatg cccagtatta aatactgggt tattttctgc tatggtgcaa aataaagata
     1261 ttaagggagt tttcgtgggg catgaccatg ataatgatta caatggcaat ttatatggtg
     1321 ttgagttatg ttatgggaga actaccggat atcgtatgca tcaaaaggat tcttttgctt
     1381 taggagccag ggtcatatta ttaaacgaaa gggaaaatgg atttaatact tggattcggc
     1441 ttgaaaacgg gatggtgctc aaaaaggata ttgatgatat tcaacaaata actctatcca
     1501 attgatgctg cattgctgct tttatattag taacataaca taataatatg tattgacatg
     1561 tcattatata aatgatataa taaaattaat tagtataggg gttgctaata ttgataataa
     1621 atgaacctat ttatgatttg aactgcttag cgcagtataa tttacatata cactctacat
     1681 attcaggctg tgcaaagcct gaaatgacag cccccaatgt tttggaatgt gcaaaaaaca
     1741 accatataaa aatcatagca tttactgatc attataacag taaagataca aaaataataa
     1801 acaataacga aaatttaaaa aaacaggcag aagcttgcaa tacaaatatt aaagttctat
     1861 atgggtcaga attgtctgca tatggaattg gaaaatatct tgatgatata gatataaatc
     1921 agcagcttgg ctatagatta tattcataca atcactacca cttggagtat tgggaacacc
     1981 ctgaagaaaa aacccctaga gggtatgtaa atcacgctct tgcattattg gagcatcttc
     2041 ttaaatccaa aagggcagat tgtatagctc atccttttat aggaaggttt atcagatgct
     2101 ttgatgatag aacattagta acaaaggaaa ttaaagataa tgaattggga gatattttag
     2161 agcttggtaa agataatgaa gttgcctggg aactaaatgt taactcgatt atagctgacc
     2221 cggaatttgg aaggcgatat tggaatattg ggaaagaagt gggagttgtt ttcaacatgg
     2281 ggacagatgc ccataggtta cagtatatta gcacattaca gtatttagaa aaaataaagg
     2341 aaatattggg agagtagttg caatctgtta atgctgtaaa atatcaagat tttcagttta
     2401 ctcttatatc ctaatacgtt acctgtacag caataataga aataacaaaa atataattaa
     2461 aagaaaggta attgtctatg caagttatga gaaatctttt ccaggtaggc ggcagcttaa
     2521 atggcataac acatgcaacc aaagaagatg caagctatga agattgcaac acatatattc
     2581 taaaaaccga taaaggtctc attatgtttg attgcggatg cggcagtact ttggagcaaa
     2641 tctacaaaaa catggaatac tggtctctgg acccttctga tattaaattc tgttttatta
     2701 cccaccctca cctggaccat gcaggagctg cttatttgct ttcaaaacaa cagataaagc
     2761 tttatgctca taaaaatacc gttgatgcta ttgcagcagg agacgaaaga tgctgtggtt
     2821 atttatatca taaaaagttc attccatgta ctttggacta tgggctttcc gatggtgata
     2881 tagtaaacat acttggtgta aacatcaaag ctatgtatat gccaggccac accatgggtt
     2941 gtacagcata cttattccaa catgaaggca aacttattgt agtcagcggg gatattatag
     3001 gtacactttt ggatggatat tttgggtgga acggttcaat agatttcaac aaacaaacat
     3061 acatagaaac tttaaggcaa tttgcaaagc tggactctga tgttatgctt ccaggccatg
     3121 gaatgatata tttccataaa ccaagaagac gagttgaaga agcattaaac atggctttaa
     3181 tacaatggag gtgagcagca tattttattg agcacttgac taaaggcagg aactttataa
     3241 atcaatagca atagaattaa tactgtaata agtgaagtgc cagagaggaa ggtgatggat
     3301 aaagagattt gaaggaaatt gtttgctgat atttcttgcg taagtctatg cttgtactaa
     3361 aaaataaagg gggattttga aatgaggaga aaaatattct gtattgtatt ggcaataagt
     3421 atatttttaa cagtatttgc agcagcttgt tcacagaaaa caactgatgc gggaaacagg
     3481 aatgattccg gaacatcttc tgctacaact ggcggaagcg ataataaaaa tgaaactaca
     3541 gaaggatata cccttcctat agtaaaagaa cctattacac tggtatttct gaaaagggag
     3601 aattcaacac caggaagctc tttcatgaat aatagacctc ttgtttggga ggagtatgag
     3661 aagaaaactg gaattaaagt caagtttgaa gcattacctt ctaatgaatt gaaggaagtt
     3721 attcaattaa gacttgcagc aaaacagaat atacctgatg taattgagat acctggaacc
     3781 caggatggaa cttacctgga aaaatattat gctgatggtg tcatcattgc attaaataac
     3841 tacatcgaca aatatgcaac aaatattaag agtatttttg aaaaatatcc tgattataaa
     3901 aaatcgttaa cgttgtcaga tggcagcatt acaggtctgg gagccttaag agcgacaaaa
     3961 tatgaatttc aggctaccgg tattagagta gatatactta aaaaattaaa cctgcagatg
     4021 cctaaaactc cggatgagct ttttaatgta gccaaagctt ttaaagctta tgacataaat
     4081 ggaaatggta aagcggacga atttggtatt acaggtaacg gagatcaatg gaaggaattg
     4141 ggcgatgcat gggggcttca ttatgtaact ggtgacggat ggacagtaag agatggaaaa
     4201 gtggtgtatg agccagttac cccggaatat caggattttt taagattttt aaggaaatgt
     4261 gttaaggagg gaatctttcc tgccgattgg aacactgttg acagcaaaac aaatgatgca
     4321 agaataataa atggtcagac tggtattatg gcaagaatga acgctttgag ttctgtttac
     4381 tacctggatc caaacggaac aatgcttaaa aactttcctg atgctgaatg gagtgttatc
     4441 cctccacttg atggacctta cgggaaaggt attcttacaa aagaaccagt agctcagcgt
     4501 tggcgtacct ttgtggttac aagtgctaac aagtatcctg gagaaacagt taaatggatg
     4561 gattatgcgg cattcagtga agaaggccag acatataata gatttggtgt tgaaaatatg
     4621 aactatagaa ttgaaaatgg taaaattata agatttacaa aggaagagca agaaaaggct
     4681 atgccggaag gagcctggtc aggatctggc tatctgcctt cagtagacac tgatgaatca
     4741 acagaaatgg gatttgctca tattgtagca ctagatcatc ctaaagttca ggaactaagg
     4801 gatttatgga aatatattga agagcctttt gtgccgccga ttcccagtgt taatgacggg
     4861 aaaaaacttg catcccttct aacagatgtt aaaactcagg tagatgaaat gttctttaag
     4921 ttcttaaacg gtgatgcgaa tattgatact gaatgggata attatgtaaa gagacttaac
     4981 gccaatggtt tggatgatgt tataagtata tatcagaaag ggtacgactc tcttaacaaa
     5041 taaccggtaa agtattacta aattcaagct ttaatgctgt taacacggca ggggtacaga
     5101 gttaatttca tccctgtcgt gttaaagaat tttataaaaa ggatggagag acattatgaa
     5161 aatagaagct ataaatagag cctctattac aacaagtata aagcaaagtc cgtttattag
     5221 aaatttcaaa aatgaaatat ttatgcatgc tttggtttta cctacggtgg tatatttttt
     5281 catatttgcg tatatgccaa tgtacggtat acttgttgcc ttcaaagatt tttcatttgc
     5341 taaagggata tggggcagca attgggtagg tttagaacat tttatgacat tttttaaatc
     5401 tatgtatttc ccccgattgg ttaaaaatac tgtattaata agtgtatatt caattatctg
     5461 gggaatgccg tttcctatta tcttcgcact tctcttaaat gaaataaaaa acaggcattt
     5521 taaaagaatt atacaaactg tcagttactt ccctcatttt gtgtccacag ttataatagt
     5581 tggtctgatg gttagtttta tgtctcctgt tgacggaatt gtaaatatat taagggtaag
     5641 atatggtcac gaagctatta attttataca gagtactaaa tggtttagga cactgtacat
     5701 aggttcagga atatggcaag gctttggctg gggttctatt atatatcttg ctgcactatc
     5761 aggtatttca aacgagcttt atgaagcggc ttatgttgat ggtgctacaa gatggcagca
     5821 gaccattcat atatccatac ctggaattat gcctacattt ataatattgt ttattctttc
     5881 tgttggagga ataatgagtg tcggatcttc gaaaattata cttatgtata cacctgcaac
     5941 atatgatgtg gctgatgtta tatctaccta tgtctatcgt aaatccctta taggcggaga
     6001 atttagtttt ggagctgcag tagggttgtt taatacagta attaactatg cattggtatt
     6061 tgttaccaat aaaataagta aaaaagtttc agatatatca ttatggtaag gtggtgaata
     6121 tagatggcca tgaaaatgag tatgggagaa aaagtatttg aaaaatttaa ctatattata
     6181 ttaactattc ttgcgttcct gacggtatac ccgtttatat atataatcag caattctgta
     6241 agcagtcttg atgctattat gaaacaggaa gtgtatttgc tgcctaaagg tatcaatatt
     6301 gactcatata aactggtatt taagcatggt ggtatattaa ctgcctatta taatacaata
     6361 tggtatacag ttgtaggaac agctattagt gtttttctaa caacaagttc agcttatcca
     6421 ttgtcgagga aaacttattc tatcagaagc cagcttatgg tatattttgc gataaccatg
     6481 tattttggtg gaggattggt tccagtcttt attttggtta atcaattggg gctttataat
     6541 acaagatggg ttatggtaat tcttggagct gtaggtgtat ccaacatggt tattgccaga
     6601 gttttttttc agactaatgt tcctgaagaa atgacagatg cagcaaggat tgatggtgcc
     6661 aacgatgtta taatatattt taaaatagcc ttgccacttt caaaggctgt tgtagctgta
     6721 cttacactgc agtatgcagt tagtcactgg aatgatttct ttaaagcaat gatttattta
     6781 aatgaaaccc atcttcaacc tttagcgcta tatctcagaa ggcttttaat tatgggtacg
     6841 gctgtagagt tcgccggtga acaggaagaa agattgcgtg aaatggatca atttgcactg
     6901 aatgcagtat cacagcaatt taaatatgct tctgtcgtta taactatgct tccgataatg
     6961 tgcttttatc ctttccttca gaagtatttt gtaaaaggta ttatggttgg agcaattaaa
     7021 gaataaaaat aaatagatag tattatcaaa gataggagtg aatattttga gtcagcaaca
     7081 gcaaaaactg ctattggaga gtaaaaaata taaggctgta ttctttgatt ttggggacac
     7141 ccttgcattt aataatgaga cctttccaga atctttatac aaaattgtaa aaagtatagg
     7201 tataggtgta gatataaatt gtttgcgttc tgtaatcaag aaagccgact atggagagtt
     7261 ggcggaagaa cggatgaaat gtagagacag ggatagctat agaaatttta gaattaagta
     7321 ctataggtat gttttaaaga tgattggata ttcacatatg gaaaagcatg cggagtatat
     7381 acataacata attaatttct ataacagaac ttacctgaaa cctgaatctt tgtatgtctt
     7441 agatacctta agaagcgata gatataaact tggagtagtg tcaaacttta gccatgcact
     7501 gccatggtac tgtgatgaac taggccttac ggaaaaactg gattttataa catattcaga
     7561 cgatataggg catgagaagc ctgaatctca tattttcaat gatgcattgg acaaggcttg
     7621 tgttaagcct gatgaagtta tacatgttgg ggattcatat aaagctgatg ttttgggtgc
     7681 gaaacaaata ggcataacac ctgtgttagt cttgcctaat tatgataagg aatatgaaga
     7741 ttgtttatgc attaataatt taattgacat tctaaacata atggggataa agcataaata
     7801 ttctgttgat ttcatataaa aaatgtacta taaatgcacc ataatcatac tcccctaaaa
     7861 ataggaaagg gaatggtacc atatggaata taaaaatgat aaattaaata tagttcaaat
     7921 aatatggcat gacctgggaa ggcacctttc ctgttatcag gaaggctcgg taagttctcc
     7981 aaaccttgat agaatggcca gagaaggtat tctttttact aatcattatt ctacaggacc
     8041 tgtgtgtgtg ccaagccgtt gtagtatcct gacgggcaga tatcctcatg cgcaggaatt
     8101 gtggcgtttc agcaataaag aagtcactct gcctaaaacg ctgaaggaag cagggtattc
     8161 tacttataga tttggattcc atgaggaaaa ggaattcaac acattgaata aagatattga
     8221 tatagaactt atgggtaaag aattactggg ttatgagtat acctggaaag aatccagaaa
     8281 gccaatggat atagcagata ggttttgtga atttctagga aaccagctta ctgacaagcc
     8341 attttttgca aatgtggctt tttatcatgt tcacaggccc aatatagaag aggtagatga
     8401 agaaactgta aataatacta aattgccagc tatactgccg gatttgccag atacatatga
     8461 atcaaaggaa gatgttgcca tactggaaaa aaagataaaa acagcagatg aagctgtagg
     8521 aaaaattttg gattgtatat gttcgcaagg tttaaaagac aaaacccttg tttatttcac
     8581 aacagaccat ggtatagatc tgccgagagc caaaatgact gtatatgatg cgggaataca
     8641 ggcagcttta atattttggg gaccggattt tatacaagcc gggaaagtag ataataatct
     8701 tcatagtcat atggatatat tccccacatt aatggaactt attaataatc ctatacccaa
     8761 gagggttcag ggaagtagtt ttgctccggt ttttacggga actgactata cacccagaga
     8821 atttgttata gtagaacgca catgggaagc ttcagatgat cctgtgaggg cgattcgtac
     8881 tccaaaatat aaatatataa gaaattattg ccccggatgg cccatacctg tgccaccaga
     8941 ttataccaga aaagttggaa aggaaatcat tgaaaagata tactcaaatc cacggccttt
     9001 tgaagaacta tatgatatcg agaatgatcc ctgtgaactg aataacctgg cgaatgatga
     9061 aaatcacaag gaagttaaag atgagatgtg gagaacactg gagaatatat tgattgaaga
     9121 cagtgatcct gtacttagta tttcagagta tatgtcttat ctaaaagagc tcgtacctgg
     9181 aaggtgggta aaggagggaa aaaggtttac ttttaatata taatagagga gtgttagaat
     9241 gtatattgta aaaaaccgta attcagcgat gaaaatagtt atatcagaaa acgcatcgat
     9301 atcggttcga cacgcagctc gtgagctaca acatttttta cggcagatga caggtgtaac
     9361 catgccaata aaaacagata atatgggtgt aaccggttca gaaatttatg tgggttatag
     9421 caaacgtacg gaacaagcag gtatattccc ccatgctgag cttggtggtg aaggttatgt
     9481 aataaagcat gtaaatggta atcttataat agccggcaat gatccaagag gtactttgta
     9541 tggtgtatat tcctttctcg aagagtattg cggctgccgc tggtatacct cggatttctc
     9601 atatataccg caatgtaaaa atattgagct tgatgataat ctggacataa cctattcacc
     9661 tgtatttgaa tatcgtgaaa cattttatta tgatagtttt gatggcgatt ttgctgtgcg
     9721 taatcatcta aatggtatgc atatgcgact aaaagatgag catggtgata aaatcaagta
     9781 tgctaaggga tattttgtac atacattttc aagattagta cctcccgaga aatactatga
     9841 tgatcatcct gaatattttg ctttacgaaa tggggttcgt gagcgagaaa acgcccaact
     9901 ttgtcttacc aatccggatg tactaaaaat atccatagaa aaaacccttg atgaactccg
     9961 taaaaatccg gatgtaaata taatttccat atcgcaaaat gatggtacac aaccctgtac
    10021 atgtccccaa tgccaggctg ttgttgaaga agaaggcagc gaagcaggtc cgatattgcg
    10081 gtatgttaat aagatagcgg aggaggtgga aaaggagttt ccgaatgttg caattgatac
    10141 tctagcatat gattattccc gcaatcccct aaaaatcacc aagccaagaa gtaatgtgat
    10201 aataaggcta tgttcctttg aatgttgttt ttctcatcct ttggatgatt gcagacaaac
    10261 agcagacttg aaaaagcgta gcaaaaagaa atccgatgat tttgcaggcg atgtagagaa
    10321 atggtctaaa atatctaata aattatacat ttgggattat gtaactaatt ttgaattcta
    10381 ccagttgttc caccctaatt tacatatcct ggcacctaat atcagatttt ttgcaagaaa
    10441 taatgtcaaa ggcgtgtttg agcaaggaaa cggacaaggc gagtatggtg aaatggctga
    10501 actcagaacc tggcttctgg caaaattact atgggatcct gattttgatg tagaaaaagg
    10561 tatgaatgaa tttctggctg catggtttgg gcctgcggca ggagcaatga gggaatatat
    10621 aaatctgatg aaagaagcac tacttgaatc aggctatcat ataagtcttt atgatgctcc
    10681 aaaagcaccc catattacag ttgaactttt atccaaagct gatgaaatct ttgatagagc
    10741 agaaaagctt accagtcaaa tgcctataga aaatcgccgt gtccgaaagg aacgggtggg
    10801 tataaaatat gcaagatttt tcaatgaaga accgggaact gatagagaag ctaaagttga
    10861 agagtttatg aattatacaa aagaccttag aattaaagta tttagtgaaa agtggggatc
    10921 tgagaatata agacgtttag ctatcagagg ggtatggccg cctaataagg aagatagtat
    10981 agtaattaga gataaataaa atcacttata attaattata tgtcggtatg cggcagaaaa
    11041 tggaggcagc tatgagtata atgagaaaaa agatactttt aaataagata aatcaactaa
    11101 aaaattgcat ttatgcatca tttgagaatt tagataactg ggaaaccagg ataggagttt
    11161 tcaaagctgc aggcaataac gaaataacaa atgaacaatg ggagaatata gctatcggta
    11221 caaggtggga ggcaaaagat gatattgtta gatggtttag acgtaatata actattcctt
    11281 ctgattttca agggaaaaaa gtgattcttg atatcgaagt gggaggtgaa ggattagtat
    11341 acataaatgg tgaaatgaaa tctgcaatta catcatatat agagccgtgg gctgcaacaa
    11401 gaacaagagt tctgttaacc ccggctgcaa agggtaatga atccttagat gtacttatcg
    11461 aagcaggact taattatatg gagtttgcag attatagaag aaaaggcatg gagtctgtac
    11521 aatatgaatt caggagtgct aaattacgcc agatagatac aaaagtagaa aagtactatt
    11581 ttgatgcaat tgtaacatat gaggttattg aggtccttga ggggaaggga tctgaaaggc
    11641 ttcccagaaa tataaaagaa atgatcacat cattggtagg gtttgacaag tatggtacat
    11701 taattcatgc gaaattagtc gatgctctgg aaagctcact ccttacttta gattttgact
    11761 tcagccgaga aagactttta gcttctatag atgaagcaga atgtatacta aacgaaaaat
    11821 taaatgaaat ccctcatttt ccacaaggac aggtaattct taccggacat tcccacattg
    11881 atacagcttg gctttggcct ataaaagaaa ccataaagaa atgttcccgt actttttcca
    11941 atacaatagc tttgatggat gaattccctg agcataattt tgcacaaagc cagccacagc
    12001 tttacgaata tacaaaaaaa tattatccgg agttgtacaa aaaaatcaga gagaaggtaa
    12061 agggtggaaa gtgggaatta ataggaaact catgggtaga gtgtgacact aatgtacctt
    12121 caggagaatc attggtaagg caaatattat atggaaggaa tttttttaaa gacgaatttg
    12181 gtaaatggtc agatatattc tggatgcctg atgtgtttgg atacagttgg gcacttccgc
    12241 aaattattaa aaggtcaggt atggaatttt tctatactgc caagcttaac aataatgatg
    12301 taaacaggtt tccatacagt cttttctggt ggcagggaat cgacggtaca agggtcatat
    12361 cttacctgca gagggcaacc tataatggtg aaataaatcc ttcatttata aatgaacttt
    12421 ggaccaatta tgatgaaaag gaattacaca gtgaggtttt aggcacttat ggatttggtg
    12481 atggaggagg aggaccaact tatgagatgc ttgaatatag taaaaggctc atgaattttc
    12541 cggggatgcc tgcgacaaaa attgatagtg cctattcatt ttttgaaaat gttaagttgc
    12601 attcttgtaa tttacctgta tggaatgatg aaatgtatta cgagtttcat agggggacat
    12661 atacaagcca ggctcatact aagaggaata accgtaaaag tgaacttttg tacagacaaa
    12721 gtgaaatagc ttcctccatt gctctaaata agttcaataa tgaatattca atagaacagc
    12781 ttacagaagg atggaagctt atacttctta accaattcca tgacattata cccggatcat
    12841 ccataaatga agtgtatagg gattgcgcag tagattataa aaagatattt gctataggat
    12901 ctgatattga agataagtct cttcaggcga ttataagcaa tgttaatttt gattatatga
    12961 atgaaaatac tgaaaaaatt agtattgaaa atgctggtat tatcgaggaa gaagctttat
    13021 acagctatat agtagtattt aactatttat catggcctgt taccggccct gtggaagctg
    13081 taatttctat taataattca aagggctata cttttgaatc gttggcactg gaagatataa
    13141 agggagataa gataccgtgc acattcaaaa agatcgatga acaaactgta gcagttagtt
    13201 ttgaagcttt agatgtacct tccatgggct acacctcata caaactggtt aaggttgatg
    13261 aagaaatgca agttggtaaa actattgata atagtacatt tgaccatata aaacgaataa
    13321 ctgttacaaa agaacttatg gaaaatcaat tcttcaagat caatgttgat aataatggta
    13381 caatttgcag catttttgat aagaaagccg ggcgggaagt actatcggaa ggtggacatg
    13441 gtaatgtatt gcagattttt gaagaccgcc cagccagaga aagcgcatgg aatattgata
    13501 tagaatacca gaaaaaaagc tgggtaataa atgaagtaaa atttgttgag gttaaagaag
    13561 tatctgaaac taaaggtgta ttgagagttg tgaaggcttt caataagtct gaaattgtcc
    13621 aggatataac catttatgca tctacaccac gtatagattt ctatacccat gttaattggc
    13681 aggagactga gaagctttta aaagccgctt ttgaggttga aattttatcc ccttgtgcta
    13741 cctatgaaat tgcctttgga agcattagac ggccaaccca ctggaatacc agctgggatc
    13801 aggcaaaatt tgaagttcct gcacataagt gggctgatct gtccgaaggc ggctatgggg
    13861 taagcatctt aaacgattgt aaatatggat acgatattaa ggataatgaa atgagaatta
    13921 ccttgctaaa agcccccgtt taccctgatc ctgttgctga taaaggcact catgagtttg
    13981 tatattcctt atatccacat ataggtgatt ggatcaatgg aaatgttgtg aatgcaggtt
    14041 atgagctgaa tgtcccatta aaagcaatat gcataaataa agataaaaac gaccagaaat
    14101 ctgcaagtca gggggttaag aatgcaaaag cttcattgcc gcctgtagca tcatttgtcc
    14161 aaatagataa agtaaatgta attatagata ctttaaaagc tgctgaagat ggtgatggtt
    14221 taatcatgag attatatgag tcatcgggca ggagaggaaa agtcaaaata agtacagagc
    14281 tagatattac caatgttaca gaatgtaatc ttatggaaga agatgaaggc cggatagact
    14341 ttaagaacgg ggaattatgt attgacatta agccgtatga gattaaaaca ttaaggttga
    14401 gaaaaagcta gaatagaaac agtaagaaac atatcagttg ataaatttag ctagaagact
    14461 gcatgagtcc gctttcccgt gcagtctcct agtaaggagc attctgaagt attttatgta
    14521 gccgggaaca ggtaacccgg ctacacgtta gcagaggaat tacgggagat attcagagaa
    14581 aaaagagaac tgctgtcagt attgccaaaa tgtgcagcag aagtaattaa aagctggtgg
    14641 agagaacaaa acaaagagga aaaatataca ctctgtccag tctcttgatt ttgtattatc
    14701 atttctaaac aaatacagtt ttagtatttc cgcaccactt ttcacgaagt ttgggttttt
    14761 ctatcttgcc tgtaggatta cggggaacct tatcaaagat aatcttccga ggccgtttat
    14821 atttaggcag atcccggcaa aattcaatta ccatgctttc tgtaagttgt cttccaggtt
    14881 tggtttctat aaccgctaca ggaatttctc ctaatcttct atctggcatt cctataacag
    14941 cagcatcctt tatatcttca tgagcctgaa ggtagttttc aatttctaca gggaatatat
    15001 tttccccgcc tgatattact acatctttct tacgatcgac caaatatata aaaccatctt
    15061 catctttttt tgccatatct cccgtataaa gccagccgtt tctcaatact tttgcagtcg
    15121 cttcaggatt tttgtaatag cccttcataa ccccagggcc tcgtataatt aattcgccta
    15181 cagttccatt ggtgacagga ttgccttcac agtctacaat ttttatctcc caattaaaac
    15241 caggtaaacc aatagctcca actttgtgaa tattttctat tccaaggtgt acacagccgg
    15301 ggcctgttga ttcactaaga ccataattgg tatcatacag atgatccgga aaatattttt
    15361 tccacctttg aatcagactt ggagggactg gctgtgcgcc aatatgcata agtctccatt
    15421 gtttaatatt ataattttta agatcaacct caccgctttc gatagcatca agaatatcct
    15481 gagcccatgg tacgagaagc cacacaatcg ttgctttttc ttcagatacc gcttcaagaa
    15541 tccattgagg cttaactcct tttaaaataa cggcttttga accaacaata aggcttccaa
    15601 accagtgcat ttttgccccc gtatgatata gaggcggaat gcataagaaa ttatcttcat
    15661 ggctctgaag atgatgacgg ttttctgtaa tgcatgccga taccagattc gcgtgtgtca
    15721 aaataatggg tttcggcata ccggtagtac ctgatgtaaa ataaagcgct gcttcatcat
    15781 aatccgaaat ttctataccc gggttatcag atggaaactg tcccataagt tcgctataag
    15841 taatatccat atagcatgtt tcttcagttt ttcttttttc tccaacgaag atataacttt
    15901 caacagaaag aagactattg cgaattgcat cgactctttc acagaattcg ggcccgtaaa
    15961 tgagtacttt cccttctgct gcctctatac attttttaat ttcttctgct gtaaacctga
    16021 aatttaaagg aacagccata gcacctgcct ttagaatgcc aaaataagaa ggcagccatt
    16081 caaggcaatt cattagaagg agtatgactt tatctcctct ggtaatacca tgttcaatca
    16141 aacaatttgc aattttattg gattgatttt caaactccag ccatgttatt tctttacggc
    16201 ttcctattgc cggctcccgt tctattagac tgacttcgtc agaatacatt cttgcattac
    16261 gtgaaagcat ttccgtaatt ggcatattaa aaactacccc cattctaccg ttcttcactg
    16321 tatatcttaa atttatatta gatattttag tttaccgaga atattttgtc aacttaataa
    16381 cctaagaggg gtgcatttaa aatttttgaa gaaatatatt ccagaagcta tcgttacgat
    16441 acttagagcc tttaaattca gctaaggg
//
LOCUS       DAOSDD010000087        16390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1432_length_16390_cov_5.391736, whole genome shotgun sequence.
ACCESSION   DAOSDD010000087 DAOSDD010000000
VERSION     DAOSDD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1432_length_16390_cov_5.391736"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..2522)
                     /locus_tag="PK733_09230"
     CDS             complement(399..2522)
                     /locus_tag="PK733_09230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ10760.1"
                     /translation="MKELLLDGLDCANCAAKIEKEVGDLDGVKAASVNFVTKKLTIET
                     IETNAKGNLEEIINEAKKIINRIEPDVSIVENDKDNSKSWKYKEDIDQSNKKQLAKLS
                     IGAVLLGIATIFDFSLWIKFSIYIISYILVGGEVVLKAAKNIARGQVFDEYFLMSIAT
                     IGAFLIKEFPEAVSVMLFYQIGEFFQNITVNRSRNSISALMNIRPDFANLIVGGEEKR
                     VNPNDVKIGDIIIVKPGEKIPLDGNVLDGGSMVNTSALTGESIPKEVGSGDEVLAGFV
                     NENGVLTIEVTKEFDESTVSKILDLVQHSTSKKAPAENFITKFARYYTPVVVIVALFL
                     AIAPPLIIPGAEFSDWVYRALVFLVVSCPCALVISIPLGFFGGIGGASKNGILVKGSN
                     YLEALNSVDTVVFDKTGTLTKGVFKVTKIKPENGITSDELLEYAAFAESYSNHPIAKS
                     ILAEYKNEVDRDKITDYKEIPGYGVKVKIGSNEILAGNIKLMTKEGTVLHFSEPTESG
                     TVLHLALNCKYMGYIVISDEIKEDSKDTILELKKLGIKKTVMLTGDNKQVGEKIAEQL
                     GLNEVYSELLPDQKVEILELLDMQKSTKGKLVFVGDGINDAPVLARADIGIAMGGLGS
                     DAAIEAADIVIMTDEPSKTVRAIKIAKKTRYIVWQNIIFALGVKITVLLLGAAGIATM
                     WEAVFADVGVTIVATINAMRTIENC"
     gene            2833..4194
                     /locus_tag="PK733_09235"
     CDS             2833..4194
                     /locus_tag="PK733_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ10761.1"
                     /translation="MGKKADLTSGNILKTLTKLALPIMGTSFIQMAYNLTDMLWIGQT
                     GSDAVAAIGTAGFFLWFAQSLIALCRIGAEVFVAQSIGEKDEESARKYAVNSVQLNIF
                     HSVIYGIFLFVCRHQLIAFFKLGEEQVINMAVTYLSYTVIGIIPTFINPVFTGIFNGS
                     GISKVPFMINAVGLVTNIILDPILIFGVGPFPKLGVVGAALATIFAQFLVTMLFLVVM
                     KKHEEIYFRVNVFQKPNFQNIKAIIRLGFPVAIQSGIFSIIGMVLGRIIAAYGPAAIA
                     VQKVGSQIESISWMTAGGFSTALSAFVGQNYGAQKYDRVIKGYKAGLLAVSIIGLFAT
                     ILLVFFAEPIFSIFIPEKEAVDLGIVYLQILGLSQWFMTLEISTQGAFNGLGKTSIPS
                     VVGIVFNALRIPAAYFLSTYTALKLNGIWWSISISSVFKGTVLVILFYHLVVRKYNKY
                     RTL"
     gene            4626..4844
                     /locus_tag="PK733_09240"
     CDS             4626..4844
                     /locus_tag="PK733_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10762.1"
                     /translation="MRYRKIFVIFWPFVAIWNLIELILRITGRLVAVILGLVLMIVGV
                     ALCFTVVGAVAGIPLAAFGFTMMIRGFF"
     gene            5004..5504
                     /locus_tag="PK733_09245"
     CDS             5004..5504
                     /locus_tag="PK733_09245"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ10763.1"
                     /translation="MKNIVLIGMPSAGKSTIGVILAKTLGMCFIDTDLVIQEKECRLL
                     QDIINTYGISRFLQIEENAVLTLKYKAAVIATGGSVVYSDKAMTHLKQDGIITYLKVG
                     YEEIERRINNISTRGIIFNKGQSLLDMYNQRMPLYEKYSDITIDCSNKDVEQIIQVII
                     DKVSSR"
     gene            5535..6026
                     /locus_tag="PK733_09250"
     CDS             5535..6026
                     /locus_tag="PK733_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HOJ10764.1"
                     /translation="MISKEKLMKTLIKGRYECYVRKNSTYNPYTKSLLYDWNFKLQME
                     DLIFTDSYRGFNPYSGVEYVYEKGIDIPIWSCDYVGYVKQGMNVPAQEVYEFLKEARG
                     NHLKNCSDNLFSNYKHENSVFQYETFFQGDVTSLLQIENFYYKNQLIAQQMTSGRLRI
                     SFP"
     gene            complement(6282..7361)
                     /locus_tag="PK733_09255"
     CDS             complement(6282..7361)
                     /locus_tag="PK733_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HOJ10765.1"
                     /translation="MAERCKMLLSQLETPALIIDLDIFEYNINKMKEMINGKLIKLRP
                     HYKSHKCSVIAHKQMKAGAKGITCAKLSEAEDLAYSGIKDILIANQIVDPVKILRLAQ
                     IARVCRITICVDNEENIIQLSQGAKYVGSQIYCLVELNSGMERCGVNSFEEVYKLAKL
                     INNSENLVFEGIQSYAGHISHEENMEKCKTTIVQLEKKLMNLKFFLENKGIKVKENSG
                     GSTGTSLIKSNSNIYTELQAGSYILMDATYKKTCPEFKNALFILSTVISSRKDVFITD
                     TGIKSCSVDQDLPQLLQFPDAAIYLNEEHSIINFKGHNFKIKDKILYIPGHCCTTVNL
                     FDKIYFVRKGQVEDVSSITSRGRSI"
     gene            complement(7408..8091)
                     /locus_tag="PK733_09260"
     CDS             complement(7408..8091)
                     /locus_tag="PK733_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ10766.1"
                     /translation="MYKYDIHTHTSEVSPCGRVNASEVVKLYKQAGYKGIVITDHYYK
                     GFFDKIDLPNWKDRLAQYLKGYRIAYNEGIQLGLTVILGIELRFIDGPSDYLIYGIDE
                     KFLLENPELYNLDLKKFRKMIEGKGMLIYQAHPFRDGFTVPGLELLDGIEIYNGNQKQ
                     NSKNDIAHSFAINNKLKMVSGSDFHIVQDLAKGGVILKEEVSTPKELADALRDNKIIQ
                     LIINGNYSS"
     gene            complement(8149..9015)
                     /locus_tag="PK733_09265"
     CDS             complement(8149..9015)
                     /locus_tag="PK733_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ10767.1"
                     /translation="MRLSTSTNIMDKVAGVSGAIPLEKCLEKCSNAGYKVMDMNFCHV
                     SGKEGYLTRNNWESWINDLAENAVKYDIEFSQSHNIIYNICDPSISNREWLEELTHRA
                     IICSGELGVKWVVMHAGTSFEKGYSALESKKKNVEYFKTYADFAQKHNTGIAIENVFD
                     FDGRQYTACVEELIDLVDSLNDFKVGICWDFSHANITNENQCLSLQRIGKRLKATHVA
                     DNNGHDDEHLAPFYGNINWEPIMKTLVEIGYEGDFTYEIQKFSENLPETVRDTMLYHT
                     VDIGNYLLSMAR"
     gene            9530..10000
                     /locus_tag="PK733_09270"
     CDS             9530..10000
                     /locus_tag="PK733_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOJ10768.1"
                     /translation="MAIDSVREYLKKWDKDNDILEFETSSATVELAAQALGVEPARIA
                     KTISFKNGESAMLIVTAGDAKIDNGKFKAEFGFKAKMLAPEETLEYTGHAIGGVCPFG
                     LKNEIPVFLDISLKRFETVFPACGSSNSAIELTCEELDIYSSNKKWIDVCKGWI"
     gene            10029..11057
                     /locus_tag="PK733_09275"
     CDS             10029..11057
                     /locus_tag="PK733_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10769.1"
                     /translation="MTLGNLKQQMLLPNKEYIINQHVNIRGLDVLLLSFTIEENKNKL
                     WLMYEDKALIEDNFHDEFHKEFKTNREEMLCRIEVRSRHINFYIKEMEIQGHIVRFSS
                     SRNGPVYESNMEARMQLQHFAEKGLIPSEWDDVRLESLVVAQYEQMEGEVAPYYDNTR
                     KLPVVLHIDRNPREAPIQCPFKVNFGKQDIGTKITYYDPVIGRESYFFINEIYSYDAY
                     DDILKKTEQIEDTEMRENMIKHHMEAMENLCPKDKNLAVIRYETADNVQLRFMMKDYL
                     EAQPVHHSSATAIGFICRSDEMGINGYKVRECALQPVDKDFNGELELELFSRSHEIPE
                     ETVYSFLP"
     gene            11080..11442
                     /locus_tag="PK733_09280"
     CDS             11080..11442
                     /locus_tag="PK733_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOJ10770.1"
                     /translation="MDIFFWITDLFIPATMISLGVIFKKWPIKKINMVCGYRTRRSML
                     SQETWDYANYRMGVLWLKWGIVLFAAVAISKIITPVEKEQLSIIHAGVELVALFASIP
                     IIETELKQKFDKSGKPKE"
     gene            11511..11819
                     /locus_tag="PK733_09285"
     CDS             11511..11819
                     /locus_tag="PK733_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ10771.1"
                     /translation="MDHVLMQICKIAEKYNADKVILFGSRARGDNSPVSDYDIAVIGE
                     KMSDLEKANFCLDVEEVETLKKIDVAFITEDIKDDFTGNIMKEGVVVYEKTRDKINEF
                     "
     gene            11785..12183
                     /locus_tag="PK733_09290"
     CDS             11785..12183
                     /locus_tag="PK733_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOJ10772.1"
                     /translation="MKKLEIKLMNFSNALQRLKEAAKELEARDASDVIRDGVIQRFEF
                     TYELAWKAAKEYLEDAGIVDKNSPKAVIKEAYAQKLISDEQTWLLMLNDRNLTTHAYK
                     EEIAKEIAARIINSYISEFENLLLKMTNYY"
     gene            12226..12435
                     /locus_tag="PK733_09295"
     CDS             12226..12435
                     /locus_tag="PK733_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOJ10773.1"
                     /translation="MQKAYVVGDIGMTDMVLARNINAKGILVLTGVGKGSLNEFRDTW
                     KHIEDDYIAENVLDAVKWIMNDLSK"
     gene            12670..13425
                     /locus_tag="PK733_09300"
     CDS             12670..13425
                     /locus_tag="PK733_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ10774.1"
                     /translation="MDKKYTEINSKCIDKWVEEGWEWGQPISHEVFEKARNNDWFVLL
                     TPTKPVPKDWFCEMKNAKILGLASGGGQQMPIFTALGAKCTVLDYSEKQLLREKEVAE
                     RENYEIETVRADITNPLPFEGESFDLIFHPVSNCYIENALPVWKECYRVLKKGGILLA
                     GLDNGINYIFDDEEKMVIRKLPFNPLKDKQLYEDSVKNNWGIQFSHTIEEQIGGQLQA
                     GFVLTDIYQDTNGVGRLHEFNVPTFYATRAIKK"
     gene            13535..13738
                     /locus_tag="PK733_09305"
     CDS             13535..13738
                     /locus_tag="PK733_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10775.1"
                     /translation="MKSYSIITGNPYEQLIIINISVSYEDFNNGNPYNTTFNVKVISG
                     DFTGVSEFEYNIKDFITKNAYRL"
     gene            13903..14514
                     /locus_tag="PK733_09310"
     CDS             13903..14514
                     /locus_tag="PK733_09310"
                     /inference="COORDINATES: protein motif:HMM:NF040123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HOJ10776.1"
                     /translation="MLNTKQKATRITLFFVIGILSGFLAKYIDTIPSNGTIGSLINII
                     SNISSRIGIWVFIATIIAEWSRTPKAGAIHVFAFFVGMLLAYYIYSMKLFNFFPTYYF
                     IRWGLIALLSPLAAYIVWFSKRNGWISALCAALPIGLLISEGYNFFYTFSPVSGFDLI
                     AAIILFCILPNDKYQYLKVSIFTILTSVFLSKFSLLSYIIGGL"
     gene            15027..15176
                     /locus_tag="PK733_09315"
     CDS             15027..15176
                     /locus_tag="PK733_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10777.1"
                     /translation="MALGIALVIAVIVGIIYGIINKNKPLGIVSVIILIMIIAVWVYF
                     YKNPY"
     gene            15269..15634
                     /locus_tag="PK733_09320"
     CDS             15269..15634
                     /locus_tag="PK733_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10778.1"
                     /translation="MKEVPELWELTGLFEIGPTYVYGKEKEIPWFYSTVNFRLKRGSE
                     TLDIIISPAYGIIAFSVFTGDREIMQVNLENVEGMKIEKLHNKETLHIIFNDNDMIKE
                     FFIETKPQIFVYCNKPQYL"
     gene            15719..15946
                     /locus_tag="PK733_09325"
     CDS             15719..15946
                     /locus_tag="PK733_09325"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HOJ10779.1"
                     /translation="MRCPYCNGEMETGILEGQRYLLWAKQPHKVSYHPKVGEVLLGEK
                     AVSSVRVDSYICKQCKKIITDYANLDGVKEG"
     gene            16008..16286
                     /locus_tag="PK733_09330"
     CDS             16008..16286
                     /locus_tag="PK733_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10780.1"
                     /translation="MTDFLMQYGEIFSTIMFFIPPILSVLILYFTKRNWIWLSIPITI
                     VVDLLVWGKAIFESSHSGIALAFLIPQVTVVAIISFVILYIEQCRKRA"
BASE COUNT         5464 a         2537 c         2934 g         5455 t
ORIGIN      
        1 gtatccataa ggatatatga gtaaatactg caatgatatt tatctttgta ccgctttaag
       61 agtttaagat aatgatcttt atcattatca gaacgataaa ggtctatttc gctaatactc
      121 ttcgacataa catgatacat accctctggg gattttcgcc tttcagctct tgccatttat
      181 tatcacctca gtatatatgt taccataaaa agaaaatatt gtcaataaaa aataatttgc
      241 aggcatttgc cggaatattt gcagaactgt ccccttaatt tctcctgaaa catttgcaga
      301 actgtcccct taatttgtga aggaaggtta aaacctttat ccgataagcc tccatctgat
      361 actcctacta ccttttctca aaaatagcaa tttcgaaatt aacaattttc tatagttctc
      421 attgcattga tagtagcaac tattgtaact ccaacgtctg caaatacggc ttcccacatt
      481 gtagcaatcc cagctgcacc taaaagaagg acggtaatct taacccccag agcaaatatg
      541 atgttctgcc aaactatata tcttgtcttt ttagcaattt ttattgctct gacagtcttt
      601 gaaggttcat ctgtcataat aacaatatca gcagcttcaa tggcagcatc agaaccaaga
      661 ccgcccattg caataccaat atctgctctt gcaagtacag gggcatcgtt tatgccatct
      721 cccacaaaaa ccaatttacc tttagtagat ttttgcatat ccagtaattc caatatctca
      781 accttctggt caggcaggag ctctgaatag acttcattca atcccaattg ctctgctatc
      841 ttttcaccta cctgcttatt gtcaccagtt aacataacag tcttttttat accaagtttc
      901 tttagttcta agattgtgtc ttttgaatct tcttttattt cgtcagatat aactatatac
      961 cccatatatt tacaatttaa agccaagtgg agaaccgtcc ccgattctgt tggttctgaa
     1021 aagtggagaa ctgtcccctc tttagtcata agctttatgt tgcctgcaag aatctcattg
     1081 ctgccaattt taacttttac gccataaccc gggatttctt tataatccgt aattttgtcc
     1141 ctgtcgactt catttttata ttcggctaaa atggattttg caataggatg gtttgaatag
     1201 ctttctgcga atgctgcata ctccaataat tcatcagaag ttattccatt ttcaggtttt
     1261 atttttgtaa ctttaaatac tcctttagta agagttcctg tcttatcaaa tacaactgta
     1321 tcaacactat taagtgcttc caaatagtta cttcctttta caagtattcc gttctttgaa
     1381 gcaccgccga ttcctccgaa aaacccaagt ggtattgaaa taactaatgc acatgggcat
     1441 gataccacaa gaaatacaag tgctctgtaa acccagtctg aaaactctgc acctggaatt
     1501 atcagtggag gagcaattgc aagaaataaa gcaacaataa caacaacagg tgtataatat
     1561 cttgcaaact tagttatgaa attttcagct ggtgcttttt tgctggttga atgctgaact
     1621 aaatctagga tttttgatac tgttgactca tcaaattctt ttgtaacttc aattgtcaaa
     1681 acaccatttt cattaacaaa gccagccaga acttcatcgc ctgatccaac ttcctttggt
     1741 atggattctc ctgttagagc agatgtattt accatggatc ctccatcaag cacattaccg
     1801 tcaaggggga ttttttcacc tggctttaca atgattatat ccccaatttt cacatcgtta
     1861 ggattaactc ttttttcctc accaccaaca atcaagtttg caaaatcagg tcgtatattc
     1921 attaatgcag aaatagaatt ccttgaacga tttacagtaa tgttctggaa gaactcacct
     1981 atctgataaa aaagcataac tgaaacagct tccgggaatt cctttattaa gaatgcacct
     2041 attgttgcta tggacattaa gaagtactca tcaaaaacct gtccccttgc tatatttttg
     2101 gcagctttta atactacttc accaccgacc aagatataac ttataatgta tatggagaac
     2161 ttaatccata acgaaaagtc aaatatcgtt gcgatgccaa gtagtacagc tccgatgctc
     2221 agttttgcaa gctgtttttt attggattga tcgatgtctt ctttatattt ccatgacttg
     2281 ctattatctt tatcattttc aacaatgctt acatcgggct caattctgtt aataattttt
     2341 tttgcttcgt ttattatttc ttctaagtta cccttggcgt tagtttcaat agtttcaata
     2401 gtaagtttct tcgtaacaaa gttaacagaa gctgccttta caccatccaa atctccaact
     2461 tctttttcta ttttagccgc acaatttgca caatctaaac catcaagtaa taattctttc
     2521 acaaatacag ccccccatat ttattattta ccttttcagt aaatcatatc ttatctattt
     2581 acatcttatt atattacaat gtatgaatat gtgttcatat gttgtaataa gattatatta
     2641 tatttgttat taaaaatcaa caacattgtt taacttttat tgagaaaaat aatttatttt
     2701 aaagaactgt cccaatattt actgaagatt agcttgtctg ctaataaaaa ttatgtttac
     2761 attttcaaga gaatagtttt ataatagaag cattcataaa ttttacattt tagcttagga
     2821 gattttgaac agatgggaaa aaaagcagat ttgacttcgg gtaatattct taaaactttg
     2881 acaaaattgg ccttgcccat aatgggaaca tcatttattc aaatggctta taaccttaca
     2941 gatatgctat ggataggtca aacaggcagt gatgcggtag cagctattgg aacagcaggt
     3001 ttttttctct ggtttgccca atcattaatt gcattatgca gaatcggtgc tgaggttttt
     3061 gttgctcaaa gcatcggtga aaaggacgaa gaaagtgcca gaaaatatgc tgttaattca
     3121 gtccagttga atatatttca ttctgtaata tatggaattt ttctttttgt atgcaggcat
     3181 caactcattg ctttctttaa gttgggtgaa gagcaggtta taaatatggc tgttacatac
     3241 ttaagctata cagtaatcgg aataattcca actttcatca acccggtttt tacggggatt
     3301 tttaatggct ccggaattag caaagttccg tttatgataa atgctgtagg actagttaca
     3361 aatattattt tagatcctat attgatattt ggtgttggcc catttccaaa gcttggtgta
     3421 gttggtgccg ctttagctac aatatttgca cagtttctgg taactatgct tttccttgtt
     3481 gtaatgaaga aacacgaaga gatttatttt agagtaaatg tatttcaaaa gccgaatttt
     3541 caaaatatta aggcaataat tagattaggg tttcctgtag caatacaaag cggtatattc
     3601 tccataatag ggatggtgct gggaaggatt attgcagcct atggacctgc agcaatagca
     3661 gttcaaaagg tgggctctca aattgaatca atttcctgga tgactgccgg aggattttcc
     3721 acagccttga gtgcatttgt tgggcaaaac tatggggctc aaaaatatga tagagtgatt
     3781 aagggctaca aagcaggact tttagctgtt tccattatag gcttatttgc aacaattttg
     3841 ctcgtatttt tcgctgaacc gatttttagc atctttatac cagaaaagga agctgttgac
     3901 ttgggaattg tgtacttaca gatattagga ctttctcaat ggtttatgac tttggaaata
     3961 tccacacagg gggcatttaa tggacttggt aagacttcaa taccttctgt tgttggtatt
     4021 gtatttaatg cattaagaat tcctgcagca tattttctat caacttatac tgcattaaaa
     4081 ttaaatggaa tttggtggtc cataagcata agcagtgtat ttaagggaac agttttagtg
     4141 attttattct atcatttggt tgttagaaaa tataataagt ataggacatt ataaaggtaa
     4201 gggtgcattt agaatttttg aagaaatata ttccaaaagc tatcgttaca gtgcttagaa
     4261 catttaaatt cagctacggg caaaattgaa ctcataaaaa tgcctgatat aaaattgaga
     4321 aacaatttta ttcaaacaac aattttgctt ctccttcgct caaattaaaa ggctttacag
     4381 tacctacact aatgcttctt ccaataattt cttcaaaaat gaaatatgca ccttaaaagt
     4441 aaaaaacaat tgaataattt attaattgaa ttttttgatg tgttatttat tattgaatac
     4501 tgttattaaa ccttatttct gcaatttatg aatgaaaaca caataagaaa tgaagactta
     4561 ttttggattt cacggtataa ttaattataa taattgataa tagttgtagt agaggggtgc
     4621 tgtaaatgcg atataggaaa atatttgtaa ttttttggcc atttgttgct atatggaacc
     4681 tgattgaatt aattcttaga ataacaggaa gattggttgc agtaatatta ggtctggtac
     4741 ttatgattgt gggtgttgct ttgtgcttca ctgttgtagg agctgtagca ggaattcctc
     4801 ttgctgcatt tggatttaca atgatgatac ggggattctt ttagaaaaag aatggatagg
     4861 gaaaagtaaa caggaaaagg aaagtggaca cttcttaaca gcattccgat gacttttaga
     4921 agcgtcctat acgaaaatgg gcagataaga gttggataag agtaagatag gagttggaaa
     4981 agagccagag ctggaggtta ttcatgaaaa acattgttct tattggaatg ccaagtgcgg
     5041 gtaaaagtac aataggtgtt atacttgcca aaacacttgg aatgtgtttt attgatactg
     5101 atttggttat acaggaaaag gaatgcaggc ttttacaaga tattattaat acatatggca
     5161 tcagcaggtt ccttcaaatt gaagagaatg cagtgcttac gctgaaatat aaagctgccg
     5221 tcattgctac tggaggtagt gtagtataca gtgataaagc aatgacgcat ttaaaacagg
     5281 atggaataat tacttattta aaggtaggat atgaagaaat cgaaagaagg attaacaaca
     5341 tatcaacaag aggaattatt tttaataaag gtcaaagtct tcttgatatg tataatcaaa
     5401 gaatgccctt gtatgagaaa tattcagata ttactatcga ctgctcaaac aaggatgtgg
     5461 aacaaataat tcaggttata atagataagg taagcagcag ataaataata aaagtaataa
     5521 ggagaattcc agagttgatt tcaaaagaaa aattaatgaa aacacttatt aaaggacggt
     5581 acgaatgcta tgttagaaaa aattctacat acaatccata tacaaaatct ttgctgtatg
     5641 actggaattt caaattgcaa atggaggacc taatattcac agattcttat agagggttca
     5701 acccctatag tggtgtagaa tatgtatatg aaaagggaat tgatattcct atctggtcat
     5761 gcgactatgt aggatatgta aagcaaggca tgaatgtacc tgcccaagag gtttatgaat
     5821 tcctaaagga agcaagagga aaccatttga aaaattgcag tgataatttg ttctcaaatt
     5881 ataaacatga aaacagtgta tttcagtatg aaaccttctt tcaaggtgac gtaacttcat
     5941 tattgcaaat agaaaatttt tactataaaa accaattaat agcacagcaa atgacatcag
     6001 gcagactgag gatatctttt ccgtaaggtg atacttcccc ctgcaaggta ctgcgatatg
     6061 ttattacctt catagctata ttccatattc aaactcatag tttctgtcgc cataatattc
     6121 agcttttgac ctttcatcat ataactttta taaaccgagg tataaagata ttaggtaatt
     6181 ccctacattt gttgtataca tgagtgagca tggagagtga tttacctaaa ttataccaca
     6241 agggaaagaa tggaaacagc ttgagaagga ataaaattat gctaaatgct tctacctcta
     6301 ctggtaatac tacttacatc ctccacttga ccttttcgaa caaaatagat tttatcaaaa
     6361 aggttgactg tagtgcagca atgacctggg atatataata tcttatcttt tattttaaag
     6421 ttatgtcctt taaaattaat aatggaatgt tcttcattaa ggtatatagc agcatccgga
     6481 aattgaagca attgtggcag atcttggtcc acactacagc tttttatccc agtatcagtt
     6541 atgaaaacat cttttcttga acttataacg gttgagagaa taaagagagc atttttaaac
     6601 tctggacatg tttttttata agttgcatcc atcaaaatat atgacccagc ctggagttca
     6661 gtatatatat tgctattact ctttatcaaa ctagttccag tgctgcctcc gctattttct
     6721 ttaaccttaa tacctttatt ttcaagaaaa aattttagat tcatgagttt tttctcaagc
     6781 tgaacgattg ttgttttaca tttttccata ttttcctcat gcgagatatg tccggcatat
     6841 gattgaattc cctcaaatac aagattttct gaattattaa ttaattttgc taatttataa
     6901 acctcctcaa aactatttac accacaacgt tccattcctg aattaagttc aacaagacaa
     6961 tatatttgtg aaccaacata ttttgcacct tgagaaagtt ggataatatt ttcttcatta
     7021 tctacacaga ttgttatacg gcatacccgg gcaatttggg caaggcgcaa tattttaaca
     7081 ggatctacaa tctggtttgc aattaaaata tctttaatac ctgagtaagc cagatcttca
     7141 gcctcactaa gcttagcaca ggtaattccc tttgcacctg ctttcatttg tttatgagca
     7201 attacactgc atttatgtga tttataatgt ggccttagtt ttattaactt cccattaatc
     7261 atttctttca tcttgttaat attatattcg aatatatcaa gatcaataat taatgccggg
     7321 gtctccaatt gacttaataa cattttacac ctctctgcca tttatttaat atccccaaat
     7381 cttggattgt tggatcaaca tctattccta tgacgaatag ttcccattta ttattaattg
     7441 aatgatttta ttatctctta aagcatctgc taattcctta ggagttgaaa cttcttcttt
     7501 taaaataaca ccaccctttg ccagatcctg aactatgtga aaatctgaac cggataccat
     7561 tttgagttta ttattaattg caaaagaatg tgcgatatca tttttggaat tctgcttttg
     7621 atttccatta tatatttcaa taccatccaa tagctctagt cccggaactg taaacccgtc
     7681 tctgaaagga tgggcttgat atattaacat tcctttacct tcaatcattt tgcggaattt
     7741 ttttagatca agattataaa gctcaggatt ttctaataga aacttctcat ctatcccata
     7801 tatcagataa tcgcttggcc catcaataaa tcttaattca atacccagta ttacagttaa
     7861 tcctaactgt atgccttcat tataagcgat tctgtatccc ttgagatatt gagctaatct
     7921 atctttccaa ttcgggagat ctattttatc aaaaaatcct ttataataat gatcggttat
     7981 tactattcct ttatatccgg cttgtttata gagcttaact acttctgaag cattaactct
     8041 tccacatgga cttacctctg aagtgtgtgt atgtatgtca tatttataca tagtaaatct
     8101 tttacctccc tttatatttt ctgtatttat ttgtattaca tctaataatc atcttgccat
     8161 acttaaaagg taattaccga tgtctacagt atgatacagc attgtatccc ttacagtttc
     8221 cggtagattt tctgaaaact tttgaatttc atacgtaaag tctccttcat agcctatttc
     8281 tacaagtgtt ttcataatag gttcccaatt tatattccca taaaacggtg ctaagtgttc
     8341 atcatcatga ccgttattgt ctgcaacatg ggttgctttt aaccgttttc ctattcgctg
     8401 cagacttaag cattggtttt catttgtaat atttgcatgg ctgaaatccc aacatatacc
     8461 tactttaaag tcattaagtg aatcaacgag atctattaat tcctctacac aagcagtata
     8521 ttgtctgcca tcaaaatcaa acacattttc aatagcaatt cctgtattat gtttttgcgc
     8581 aaaatcagca taggtcttga aatactcaac attctttttt tttgattcta aagctgaata
     8641 tcctttttca aagcttgtac cggcatgcat tacaacccac ttaactccaa gttctcctga
     8701 acagataata gcacgatgtg taagttcttc taaccattcc ctattactga tacttggatc
     8761 acaaatattg taaataatgt tatgggactg cgaaaattcg atatcatact taactgcatt
     8821 ttctgccaaa tcgttaatcc aagattccca attatttcgt gtcagatatc cctctttacc
     8881 tgatacatgg caaaaattca tatccataac tttatatccg gcatttgaac atttttcaag
     8941 acatttttcg agaggaatgg ctccagatac tccagccacc ttatccataa tattcgttga
     9001 tgttgaaagc ctcatggtat ttccatcctt ccttattata tattcataat aatatattca
     9061 tgttttttat tatagcattt tgtttcagtt aagcatttgg acttattcct ctttattcat
     9121 cacatataca agaagtttag cataaaaata aatgatatgc aaagtcttgt acttttaacc
     9181 agttcattat ttcattgaaa gacacatgaa aatagtgtac acttttaatg gaggtgttgc
     9241 aaatgctcga aaacataaca ttcactgctg ataaggacat aattgaaaaa gcacgcaaga
     9301 aagcaataga gaataatata atcctgaatg agctttccag agaatgactt tctaattatt
     9361 ttatgaatat aaatagtaat aaaattaagc atttcattga taatgtatcc tattataagt
     9421 aatatatgta cagaaattga tgctgatgta aatagcagtt atcaaataat aaaaaaagta
     9481 tttccaataa agcatgatgg aataagcatt tatgaaagga ctaattatta tggccattga
     9541 tagtgtacgt gagtatttaa aaaagtggga caaggacaat gatatattgg aatttgaaac
     9601 atcaagtgct acagttgaac tggcagcgca agctttaggg gtcgaacctg caaggatagc
     9661 aaagactatt tcatttaaaa atggagaaag tgcgatgctg atagttacag cgggagatgc
     9721 aaaaatagat aacggaaaat ttaaagcgga atttggtttc aaagcaaaga tgctggcacc
     9781 tgaggagact ttggaatata ccgggcatgc tattggtgga gtttgtccct ttggtttgaa
     9841 aaatgaaata cctgtattcc ttgatatatc acttaaaaga tttgaaactg ttttccctgc
     9901 ctgcggaagc agtaattctg caattgaact tacatgtgaa gagcttgata tatattcctc
     9961 gaataaaaag tggattgatg tttgcaaagg gtggatttaa ttttcgaaaa aaaggatggg
    10021 gaaaaataat gacattaggt aatttaaaac agcaaatgct gctgccaaat aaagaataca
    10081 ttatcaatca gcatgttaat atacggggtt tggatgtttt gctattgagt tttacaatag
    10141 aggaaaacaa aaacaaattg tggttgatgt atgaagacaa ggcattaatt gaggataatt
    10201 ttcatgacga attccataaa gagtttaaga ccaatcggga agaaatgctg tgccgaattg
    10261 aggtgagaag cagacatata aatttttata taaaggaaat ggaaatccaa ggacatatag
    10321 tcaggtttag ttcatcacga aatggtccgg tttatgaaag caatatggaa gcgagaatgc
    10381 agttacagca ctttgcagag aaaggcctga tcccttcaga atgggacgat gtaagattag
    10441 aaagtcttgt tgtcgctcaa tatgagcaaa tggagggcga ggttgcccca tattacgata
    10501 acaccaggaa attgcctgtt gttttgcata ttgacagaaa tccaagagaa gccccaattc
    10561 aatgcccttt taaagttaat tttggcaaac aggacattgg aacaaagatt acatattatg
    10621 atcccgttat tggaagggaa agctactttt ttataaatga aatttattcc tatgatgctt
    10681 atgatgatat cttaaaaaag acggagcaaa tcgaggatac cgaaatgcgt gaaaatatga
    10741 taaaacacca tatggaggct atggaaaacc tatgcccgaa ggataagaat ctggctgtta
    10801 ttagatatga aacagcagat aatgtccagc ttcgttttat gatgaaagat tacctggagg
    10861 cacagccggt gcatcatagc tctgccactg ccattggatt tatatgcaga tctgatgaaa
    10921 tgggaatcaa tggctataaa gtgcgagaat gtgcgttgca gccggtggat aaagatttca
    10981 atggtgaatt ggaactggaa ttgttttcac gatcacatga aataccggag gaaactgtat
    11041 atagtttcct accttaaatt tgtaaaatgg ggtggtgtaa tggatatatt tttttggata
    11101 actgacctat ttattcccgc aactatgatt tctctgggag taatttttaa aaagtggcct
    11161 attaagaaaa taaatatggt atgtggttat agaacaagac gttctatgct ctctcaggaa
    11221 acatgggatt atgcaaatta ccgcatggga gtcttatggc taaaatgggg cattgtatta
    11281 tttgcggcag ttgcaataag caaaataatt acacctgttg aaaaggaaca attaagcata
    11341 attcatgcgg gcgtcgaatt agtagccttg ttcgcttcca tacctattat tgagacggaa
    11401 ttaaaacaga aatttgacaa gtccggaaaa cctaaagaat agatgttgga tgtgcacagt
    11461 aagaagtaca ttttacgttt agtacaaaat agtgaatgga gaattataac atggatcatg
    11521 ttttaatgca aatatgtaaa attgctgaga aatataatgc ggataaagtt attttatttg
    11581 gttcacgggc aagaggggac aattctcctg ttagtgatta tgatattgca gttattggag
    11641 aaaaaatgtc cgatttagaa aaggcaaact tctgtttaga tgttgaagaa gtggaaacac
    11701 ttaaaaaaat tgatgtagcc tttataactg aagatataaa agatgatttt acaggaaata
    11761 tcatgaagga aggcgtagtt gtatatgaaa aaactagaga taaaattaat gaattttagt
    11821 aatgctcttc aaaggcttaa agaagcagcc aaagagcttg aagcacggga cgccagtgac
    11881 gttattcggg atggtgttat ccaaagattt gaatttacat atgaattagc atggaaagcc
    11941 gcgaaggaat accttgagga tgcaggaatt gtagataaaa actcaccaaa agcagttatt
    12001 aaagaggcgt atgcacaaaa gcttatttct gatgaacaaa cctggcttct tatgttgaat
    12061 gatagaaacc ttactacaca tgcatataag gaggaaatcg caaaagaaat agctgctaga
    12121 ataattaaca gttatattag tgaatttgag aatctattat taaagatgac taattattat
    12181 tgattgacat aacaggcagg tgattttcat ggatttaaaa ttgatatgca aaaagcatat
    12241 gtagttggag acattggaat gaccgatatg gtcttagcaa gaaacattaa tgcgaaagga
    12301 attcttgttt taacaggagt tggaaaaggt agtttaaatg aattccggga tacatggaag
    12361 catattgagg atgactatat agcagaaaac gtgttggatg ctgttaagtg gattatgaat
    12421 gacttatcaa aatgatgcta aaactacttg aagatattat atgtattgat ttgacttgtg
    12481 acattcaaat gcagggcttc tatgaacaat ttggaatgct gaaatctcat ggtatggttt
    12541 ttcgcaagta tctcaaacaa acctaatatt tcacattata aattacggtg cataagtaga
    12601 atattatgca gtagaatact aaagggaatt aatgattgct aatattttct aatgagggga
    12661 gaggtttata tggacaaaaa atatactgaa attaactcga agtgtattga caaatgggta
    12721 gaagagggct gggagtgggg acaacctatt agtcatgaag tttttgaaaa agcaaggaat
    12781 aatgattggt ttgtgctatt aacacccaca aaacccgtgc caaaagattg gttttgcgaa
    12841 atgaagaatg ctaaaatatt gggcttggct tctggcggtg gtcagcaaat gccaatattt
    12901 acggcattag gtgcaaagtg tacggtactg gattattcag aaaaacaatt attgagagaa
    12961 aaagaggttg cagaaagaga aaactatgag attgaaacag ttagagcaga cataacaaat
    13021 cctttgcctt ttgaaggtga atccttcgac ttgatttttc atccagtttc taattgctat
    13081 attgagaacg ctctccccgt ttggaaagaa tgttatagag tattgaaaaa aggtggaatt
    13141 ttattggctg gattggataa cggcattaat tatatttttg atgatgaaga aaaaatggta
    13201 atccgcaaac tgccatttaa tccattgaag gataaacaac tatacgagga ttcagtaaaa
    13261 aataattggg gaattcagtt ttcacatacc attgaagaac aaattggagg acaattgcaa
    13321 gcaggatttg tacttacaga tatataccaa gatacaaacg gcgtaggtag acttcatgaa
    13381 ttcaacgttc caacttttta tgcaacaaga gcaattaaaa agtaagattc tcaatatagg
    13441 ctaaagtgat tttaattcac attttttata tgttgtgaca gaacagtatt aaaatgctaa
    13501 ccaagtacaa tgtaagtttt gagaggtaaa aaacttgaaa tcgtactcta ttattacagg
    13561 aaatccctat gagcaactaa ttattatcaa cattagtgtt tcctacgaag atttcaataa
    13621 tgggaacccg tataatacaa cttttaatgt taaagtaatt tcaggagatt ttacaggggt
    13681 ttctgaattt gaatacaaca ttaaagattt tattacgaaa aatgcatata gattgtgacc
    13741 attttcctcc aattatacca aaagattttc attttgatgt gtacgataga taattttaat
    13801 agttatgaag tatatgagat taatataatc acctggtaga taatagagaa tattattgag
    13861 gagtattaaa attaatactg ttagtggaag agggatgaag ccatgctaaa taccaaacaa
    13921 aaggcaaccc gaattacatt attctttgtt ataggaatcc tttctggatt tctggcaaaa
    13981 tatattgata ctataccttc taatggtacg attggcagtt taattaacat aattagtaat
    14041 ataagttcca gaataggaat ttgggtcttt attgctacta ttattgctga atggagcaga
    14101 acacccaaag caggagcaat tcatgttttt gcttttttcg tcggaatgct gctggcttac
    14161 tatatatatt ctatgaaatt atttaacttc tttcctacat attatttcat tcgctgggga
    14221 ttaattgctt tattatctcc gctggctgcc tatattgtat ggtttagcaa aagaaatgga
    14281 tggatttcag cattatgtgc tgctcttcct attgggttat tgatttcaga agggtacaac
    14341 tttttttata ccttttctcc agtttcaggg tttgatttaa ttgctgctat catacttttt
    14401 tgcattttac cgaatgacaa atatcaatat ttaaaagtat cgatatttac aatattaaca
    14461 tcagtatttc tcagtaaatt tagtttgttg tcctatataa ttggtgggtt atagaagaag
    14521 tatacggcgt gtaaagagtg ctattgcgaa aacccaaaaa atgccacttt taaggctgag
    14581 cattgtttaa atgctctttt ttttacagtt catttttatg tttttataat tggcagaagt
    14641 gtagtatttc tattgccgag cgtaatttgc tttatttctg ctgtatattt taaaaacaaa
    14701 agtatataaa ataagcattg cctttatatg aaactaagcc atattcttta tatgagctaa
    14761 ggtattgacc atgactaata ttgctgccat gatagaaata agaaatagat ataggagaca
    14821 ggttgtgggg gagattaaga gggcgctaga ggaactaggt gaaaggtagc aaggtgtaag
    14881 aaagtaggtg cttttacggg gtgtatggtg taagataaag gtatatggta cacattagta
    14941 gaaaaatgcg accttattta aatagaattt gaatccttta aaaggtggcg gtaaacggta
    15001 atatcaaaat aaaggagtgg tgaggtatgg cgttaggcat tgctcttgta atagccgtaa
    15061 tagtaggtat aatctacgga ataattaaca aaaacaagcc tttaggaata gtttctgtaa
    15121 ttattttgat tatgattatt gctgtatggg tgtattttta taaaaaccct tattaaagtt
    15181 cagatttgaa gaatactatt ttgtaacaca acattctaat tttatgtggc aatggtaaaa
    15241 tactctgaac caaaggagta agaattaggt gaaagaagtg cctgaattat gggaacttac
    15301 gggacttttt gagataggac ctacatatgt ttatggaaaa gaaaaagaga taccgtggtt
    15361 ttatagtacg gttaatttta ggcttaaaag aggaagtgag actttagata ttatcatttc
    15421 tcctgcttat ggtattattg ctttttcagt gtttacaggt gatagagaaa taatgcaagt
    15481 taaccttgaa aatgtagaag gaatgaaaat tgaaaaattg cataataaag agactttgca
    15541 cattattttc aatgataatg atatgataaa agaattcttt atagagacaa aaccgcaaat
    15601 ctttgtatac tgtaataaac cacaatattt ataatattac aaattcttct tattatgtaa
    15661 cgtaaaaatt atttatataa cacgaatgtg aacagaaatt tgaatggagg taaaattaat
    15721 gagatgtccc tactgtaatg gtgaaatgga aactggtata ttagaaggac agaggtatct
    15781 tttatgggca aaacaaccgc ataaagtatc ctatcatcct aaagtgggtg aagtcttact
    15841 tggagaaaaa gcggttagtt ctgtcagagt agattcctat atttgcaagc aatgtaaaaa
    15901 gattatcaca gactatgcta accttgatgg tgtaaaagag ggatagatag tacatattgg
    15961 taggaatatg taaagcaagg attatttatt gatgaggtga tgatgtcatg acagactttt
    16021 taatgcaata tggcgaaata ttttccacaa ttatgttttt tataccacca attttatcgg
    16081 tgctaatatt atattttacc aaaaggaatt ggatatggct gtctatacca attacgatag
    16141 tggttgattt attagtttgg ggaaaggcaa tttttgaatc atcacatagt ggaattgcac
    16201 ttgccttttt gattccacag gttacagttg tggcaatcat ttctttcgtg attttatata
    16261 ttgaacaatg tagaaaaaga gcataatctt tagatgctta catgtaacgt tcttcttata
    16321 ttacatatta gtaagatatt gtgcaacact aattttaaaa aaacaaaaga tttatatgga
    16381 ggagtgaaat
//
LOCUS       DAOSDD010000088        16345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1436_length_16345_cov_5.101044, whole genome shotgun sequence.
ACCESSION   DAOSDD010000088 DAOSDD010000000
VERSION     DAOSDD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16345
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1436_length_16345_cov_5.101044"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1413
                     /locus_tag="PK733_09335"
     CDS             178..1413
                     /locus_tag="PK733_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOJ10781.1"
                     /translation="MQTAYKLQRSIQKERFEILKESIHDYAQIKDSVKTGIEDRNNII
                     KQKKLIMEYLNATEEDWNDYKWQLKSRFTNLESVAEILNLSKEEIDEINTVGSKYRYA
                     ISPYYLSLIDPKNKKCPIKLQSIPSILELDEKGVLDPMNEKDSSPVELVTRRYPDRLI
                     IKVTNMCGMYCRFCQRRRLIGEFDTNAPKNKLVKAIEYVRKNREIRDVLITGGDAFMM
                     PDSEIEWLLQELRNIEHVEIIRFGTRTPVTLPQRITDDLVKILKKYHPIYVNTHFNSP
                     REITCEAKTACEKMANAGIPLGNQMVLLKGVNDDPFIVRKLNQSLLTIRVKPYYIFHP
                     KTVKGTSRFWVNIQTGLEIMESLRGRTSGMAIPTYIVNGPGGLGKTPILPNYMLFIGE
                     DRAVMRNWQGEIFEVENGI"
     gene            1643..2671
                     /locus_tag="PK733_09340"
     CDS             1643..2671
                     /locus_tag="PK733_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10782.1"
                     /translation="MSKLKFGIIGCGLISNWHADAILKINGAELSGATDVSEKSRQAF
                     ASKYNIKAFDSIEELLASDVDVVSVCTPSGLHAPLAIKVANAGKHIIVEKPMAITLEQ
                     ADQMIEACEKNNVKAAIISQMRFTPAVRKLKEAIDTGVLGRIVSGDMYMKFYRSQEYY
                     DKGGWRGTWKMDGGGALMNQGIHGVDLLRYVMGPVKSIHALTRTLARKIEVEDTAAAV
                     MEYANGALGVIQATTSIYPGLPRRMEISGDKGTVILEESSIVKWCVEGQEVPEDINIG
                     SPQDSSASDPAAFGVEGHVMQISDMVDAINNNRRPLVDQYEGRKPVELILAIYESSKT
                     GKTIELEF"
     gene            2980..4272
                     /locus_tag="PK733_09345"
     CDS             2980..4272
                     /locus_tag="PK733_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10783.1"
                     /translation="MTIMIKNTNSNFEREPLLAPFGFKGGYLSELWQVVALLESESGK
                     RGTGLGVQSVLWSDAAVFAANSQAAGNSAMFLMTEFALQKARDLSFKTPIELLEQLLP
                     LTYEYGRKITNNDKLRMTFALNSLVAVDNAAWIVYCKEKGISNFDDMIPEEVKPALSF
                     KHDKLASIPLITYGVSMDGIKKTVDDGYFFLKIKIGSDPERDGNLNKMLEWDKQRLTE
                     IHNAVKDIKIPYTENGRIPYYLDANGRYDSRDRLMKLLDYADKIGALERIILLEEPFP
                     EEYKIDVSDVPVRLAADESAHSDMDAIERIDLGYSAIALKPIAKTMSMSFKIAKIAHE
                     RGVPCFCADLTVNPVMVDWNKNVAARLSPLPGMKIGVLESNGHQNYCNWKKMKEYHPC
                     YGAEWIETVDGIFSLDEEFYLESGGIYKESKHYESLVK"
     gene            4278..5384
                     /locus_tag="PK733_09350"
     CDS             4278..5384
                     /locus_tag="PK733_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ10784.1"
                     /translation="MFKNSLSSENLKSILEAFSIKDFDEVLPIANGTANKNYLVVASP
                     SLVIRERSSKYSSEEQMLFEEEYLNHVFSKGIPVPVPLKTLNHLCWYSCHNRVYQVYP
                     FIGSNPGDNRDYNVGDGKGNCADSIEGDGENDYNGKNDVEEAGGFLGRLHSAVNGFTP
                     QTLRNLPRYDDPSIIIHEVSRIIEKSINTITVEERKTLEYILEQAKLVNMHINDEVYY
                     SLPKLLIHGDYHPANVKYMNGKISGLFDFDWVSLQPRIRDITDGIIYFSSKRNENLTG
                     NNIFSLATGYIFDLEGMRLFIKGYSQNVSLPVLEEEIKNIPYFIRARLMHSRVQALKK
                     IPEALSIKMLTEGMKHLLSWIDENQNRLIENLMD"
     gene            5640..6485
                     /locus_tag="PK733_09355"
     CDS             5640..6485
                     /locus_tag="PK733_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10785.1"
                     /translation="MHILLPVDVMWISKIKYDHKVLEKHTHQFYHMFFIRKGECIMHV
                     AGKEYTADENEVYFCPPEIVHGMIAQGNIPLSTIEVKFTVLDKNFAEKLSELDYRVKC
                     KPHNMYLKLEELVMEGLHKPVLYKEIVNTGFTRVLLELMRNEENPFSINIDRVKNSMN
                     NEIKDKEGSSFVLGKVLDYMNRMYAEDITLQNLADVGAVSSAHLNKLFRSTFNISPMQ
                     YINNMRLDKAKELMMYSDFNISQISELVGFESIHYFSRYFKKKEGISPSEFRNGVKDN
                     IYVYL"
     gene            6689..7699
                     /locus_tag="PK733_09360"
     CDS             6689..7699
                     /locus_tag="PK733_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10786.1"
                     /translation="MRSLKVGLIGCGGFAKGMHIPILKSNPKYTIHAAMDVYEDAAKE
                     VAESTGAAYWTKDVDRLLSDREIDVVFITTRHDSHAELSIKAANAGKHVMCEKPMGLN
                     RDQCKAVVEAVKRSKVKYTVGYNRGMAPMVTKARDLIDNELHGSKKMIYHRIQAPFRE
                     SHWTHLPEIGGGRFVGEGCHIFDLLCEIVQAPPVTVFASGGTFLNPEKVKIPDSGVVT
                     ITFADGSIGTTLINSAGCPDFPKEATEIYCDGKAIFINDFTKMEYYGFEGHKKTYMEF
                     DKVDKGHAIELDMLADAIVYDTESPNGLVKASRAAVISFMVNESIKTGAPVQINEKDY
                     IW"
     gene            7693..8658
                     /locus_tag="PK733_09365"
     CDS             7693..8658
                     /locus_tag="PK733_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10787.1"
                     /translation="MVKKGRISDMIRIGVVNIDVSHPKTFSEYLKKGNRARYVAVYND
                     GFRGDDEVQGFIKNYNLEKRCESVEELADMVDVGFIQGCNWDKHIKYIKPFIERKKPV
                     FIDKPIVGSLADCRKIEQLVAEGAVILGSSSVRYAEEVVKFASTSEEERGKILNIFGT
                     SGVDEFNYSIHIVEAIGGIAGTGAVSCTFAGRSEVDGKMCETFFVKFANGITAVYNAF
                     QGLWQPFEMVIMTTKGTYQFRIDTGKIYGALLDRICDYMETGNNTLAPIEALTESVKI
                     MLAGRISREKSGVEVKLLDIPEDDPGYDGDRFEKGYASKAAKTYL"
     gene            8836..10068
                     /locus_tag="PK733_09370"
     CDS             8836..10068
                     /locus_tag="PK733_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ10788.1"
                     /translation="MMYFAVALGILFCSFWVAQLIFVYLHLHWTNTIIESNDGDADVP
                     VTVIHPIKDLDFEFEKNFESWMIQNYRGKVQHIFSFQEPDDPAIKVVKKLKERYNDRD
                     IEIIVNPVMEGLNGKSSNMVNGMKLAKYDIVIFIDSDIRIKPDFIAKMTRPLADEKVG
                     ITTCGQINIGGSDFWTRFFTFVQNSETDFIWAFLTKMGFDVGATGAAFGMRKKLLQEI
                     GGLEAFGGSLLEDLHLGNTLYKMGYKLVLGPFLECHVDRIAREKSFNYAKRIAVGIKT
                     HIAVDLPAFIIILSWYWVLFIIAVLTQNEKLLYLSLFYMAFRTVHGLFHRKVTMNKVL
                     SVDLPMALFFDLFGTFYLLYAFNRPYVTWRGIEYKVKKGGYIENVSFNEAAILDEDFI
                     NEQFIDVDVGDEVEDVQK"
     gene            complement(10240..11373)
                     /locus_tag="PK733_09375"
     CDS             complement(10240..11373)
                     /locus_tag="PK733_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10789.1"
                     /translation="MDIQVIPVRSMTDLKKFIMLPWGIYKGNSNWVPPLIRDMKKNLA
                     PLINPDKREKECELFIAMLEGKPVGRIYAGINRLLNEKKNAQMGYFSLFECIDNIEVA
                     KSLFDTAFSWFKEKGINLVRGPVSTEGADLDESKGLLIDCFDRPPVLMNSYNPEYYIR
                     LFEEYGLEKDYDVFAYYLDPDKIFSKNPSKVLDYAKRKYDFRVDSLNLKNLDKELEDI
                     KYILDLAIPAEWSDLAAPDMDEVRSIAKSYISYVDPDLVAIARTNNNRAIGFAVALPD
                     YNQLLIHLNGRINPISILKYAYYKKRINGARVFIMFVIPEYRKKGVSFAVYHYAFNNG
                     VKKGYTWGEGSTIGEENVNMRNDIESIGGQHYKTYRIFRKELA"
     gene            11746..12039
                     /locus_tag="PK733_09380"
     CDS             11746..12039
                     /locus_tag="PK733_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOJ10790.1"
                     /translation="MNTKLRDPLIDKLFEAVLLLKDAEECYKFFEDICTIAEIKALAQ
                     RLEVARLLREKKTYNDISDLTGASTATISRVNRALNYGADGYNMILDRLDSKN"
     gene            12163..14892
                     /gene="secA"
                     /locus_tag="PK733_09385"
     CDS             12163..14892
                     /gene="secA"
                     /locus_tag="PK733_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOJ10791.1"
                     /translation="MLKFIEKMFGNYSEREIKRLMPLVDAIEELEPDIEKLSDENLKG
                     KTEEFKERLSNGATLDDILPEAFAVVRETSKRVLGMRHFSVQLLGGIVLHQGRISEMK
                     TGEGKTLVATLPVYLNALEGKGVHVVTVNDYLAKRDSEWMGKIYNFLGLSVGLIVHGL
                     DNNQRRKAYASDITYGTNNEFGFDYLRDNMVIYKEDMVQRDLTYAIVDEVDSILIDEA
                     RTPLIISGAGDKSTDLYSKANSFVNRLKPVVFTETDDKEFMDDVDADYIVDEKANSVT
                     LTEKGIKKAESYFGLENLSDPDNMTISHHINQALKARSLMKRDKDYIVKDGEVIIVDE
                     FTGRLMYGRRYSDGLHQAIEAKEGVKVERESKTLATITFQNYFRMYKKLAGMTGTALT
                     EEEEFRTIYKLDVIEIPTNKPMIRADNSDVVYKTEKGKFNAVIDEIAECNKRGQPVLI
                     GTISIEKSELLSAMLTRRGITHNVLNAKHHEKEAEIVSQAGKYSAVTIATNMAGRGTD
                     IVLGGNAEFLAKMEMRKQGYPSDVIVEAVGFNETDDEVILESRKTFRELYEHFKNQTD
                     GEKEKVIAAGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYISLEDDLMRLFGS
                     DRLSGIVDALGLEENQPIEHRMLSNAIESAQRRVEGKNFDIRKHVLQYDDVMNKQREV
                     IYGQRRNVLDGENLKDSFIKMIENISEQIVAAYCSESPHPDHWDLDGIISYVENILLP
                     HGTFKIQDEDMEFLTKDSLKEKILEMTMETYEAREKELNSELMRELERVVLLRVVDEK
                     WMDHIDAMDQLRYGIGLRAYGQRDPVIEYKFEGHEMFEEMIRNIQEDAVKIVLKSHID
                     TGRALERRKVAEPVNAVHNDQPGGPVVRSQKAGRNDPCPCGSGKKYKKCCGSN"
     gene            15019..16335
                     /locus_tag="PK733_09390"
     CDS             15019..16335
                     /locus_tag="PK733_09390"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOJ10792.1"
                     /translation="MTYDEALKYIHGTHKFGVKLGLDNVAKLLELLDNPHQKLKYVHV
                     AGTNGKGSTVAFISSILIEAGYKVGIYTSPYIEHFTERISINHKEISEGDLARITEYI
                     KGKVQEMVSMGYNHPTEFEIVTAIAFQYYYENKCDIVVLEVGLGGRYDSTNIIGTPLV
                     SVITSISYDHMSILGNTLSKIAFEKAGIIKKDGDVVLYPQSQEVEEVIAKTCEEMHST
                     LRKADFSKIISLEYGADGQYFNCGLYEGLKTKLLGEHQLKNAAVAITACEVIMDKECK
                     ITENTGCKITVDAIRNGIKKATWPGRMEIVSKQPLFLIDGAHNEDGARVMVDTLLKYF
                     PGKRKVFIMGVLKDKEYEKIVEIVAPIANQFITVTPLSDRALPSDDLAETIKRYCKNV
                     IICDKIEKGIETSLRISDSNAIICAFGSLYYIGEVRRYFELIKLTI"
BASE COUNT         5599 a         2407 c         3562 g         4777 t
ORIGIN      
        1 gataatttta atagcatcgg gaagatgttg tgtactacta atttattatt cattatcata
       61 agcaccaggt ttaattataa acattaggcc tgaattataa acatcaggtc ttaagcttcg
      121 gtcaaataag caggctgtaa agcttgcatg catttaagtt aaaaaggggg ttttcacatg
      181 caaactgcat ataaattaca aagaagtatt caaaaggaaa gatttgaaat attaaaggaa
      241 agtattcatg attatgcaca aattaaagat agtgtaaaaa caggaattga ggataggaat
      301 aatattataa aacagaagaa gctgattatg gaatatctta atgccacaga agaagattgg
      361 aatgattata aatggcagct gaaaagcagg tttaccaacc tggagagcgt tgctgaaata
      421 cttaacctct caaaagagga aatagatgag attaatacag taggatcaaa atacaggtat
      481 gccatatctc catattattt atctttaata gatcctaaaa ataaaaaatg ccctataaag
      541 ctgcaatcga ttccttcaat tttagaactt gacgaaaaag gtgtacttga tcctatgaat
      601 gaaaaagatt cctcacctgt ggaacttgtt acaagaaggt atcctgacag gctgattata
      661 aaggttacca atatgtgcgg catgtattgc aggttctgtc aaagaagaag actaatagga
      721 gagtttgata ctaatgcacc aaaaaataaa ttggtaaaag ccatagaata tgtaagaaaa
      781 aacagagaaa tcagggatgt tcttattaca ggaggagatg catttatgat gccggattcc
      841 gaaatagaat ggttattgca agaattaaga aatatcgaac atgtagaaat aatcaggttc
      901 ggaacaagaa ctcccgtcac actgccgcag cgtattacag atgacctggt aaaaatactg
      961 aaaaagtacc atccgatcta cgtaaataca cattttaaca gtccgagaga aataacttgt
     1021 gaggcaaaaa ctgcttgtga aaagatggca aatgcaggta ttccactggg taatcaaatg
     1081 gttttactaa aaggggttaa tgatgatcct ttcattgtaa gaaagttaaa tcaaagcttg
     1141 ttaacaataa gagtaaaacc atattatata tttcatccta aaaccgtaaa agggacatcg
     1201 cgtttttggg taaatataca aacagggctg gaaataatgg aatcattgag aggacgaact
     1261 tcaggcatgg ccattcccac atatattgtt aatggaccgg gaggacttgg caaaacccct
     1321 atattaccaa attatatgtt attcatcggt gaagaccggg ctgttatgcg aaattggcag
     1381 ggagaaatat ttgaagtaga gaatggcata tagaatatga aattgtaaat gctaaaataa
     1441 aagtgataaa tgcaaagatt cttgtttgga aactatatat aattaatata ttgttctatt
     1501 taatatgata ttcactcgta tgataatggc aataattaaa ggtaaagaat tttttgcggc
     1561 attttcttta tcaaaattaa cctgtaaaaa aatattataa ttattattat atgttacata
     1621 gtttaatgga ggtgcgtttt ttatgagtaa gttaaaattt ggaatcatag ggtgtggctt
     1681 aatttctaac tggcatgcag atgctatatt aaagattaat ggagctgagc tttcaggtgc
     1741 tacagatgta agtgaaaagt ccaggcaggc ttttgcatcc aagtataata ttaaagcctt
     1801 cgattcgatt gaagagctcc ttgctagtga tgttgatgta gtatctgttt gcacaccaag
     1861 cggcctgcat gcaccccttg ctattaaagt agcaaatgcc ggtaagcata ttattgttga
     1921 aaaaccaatg gcaattacgc ttgaacaggc tgatcaaatg attgaagcat gtgaaaaaaa
     1981 taatgtaaaa gctgcaataa tatcacagat gaggtttact cctgctgttc gcaagcttaa
     2041 agaggcaatt gatacaggcg ttttgggaag gatagtatca ggagacatgt atatgaaatt
     2101 ttaccgttcc caggaatatt atgataaggg gggatggaga ggaacatgga aaatggatgg
     2161 agggggagcc ttaatgaatc agggtattca tggggttgat ttattacggt atgttatggg
     2221 ccctgtaaag tctattcatg ctcttacacg aactcttgcc agaaaaattg aggtggaaga
     2281 tacggctgct gctgtgatgg aatatgccaa tggcgctctt ggagttattc aagccactac
     2341 ttccatatat cctggactac ccagaagaat ggaaataagt ggtgataaag ggacggtcat
     2401 tcttgaagaa agttctattg taaagtggtg cgtagaggga caggaagttc ctgaagatat
     2461 aaatatagga agcccgcagg acagttctgc cagtgatccg gcagcttttg gagtagaagg
     2521 tcatgttatg cagataagcg atatggttga tgctataaat aacaatagaa gacccctggt
     2581 tgatcaatac gaaggaagga aacctgtaga actaatttta gcaatatatg aatcctcaaa
     2641 gaccgggaag actatagaat tggagtttta gctgcagcag cagtacttaa tttaagagca
     2701 attgaaggta ttattaagga gcatggggat aggaaacatc ccggctctct ttttttaact
     2761 gcaaacaagg gaatatgatt tgatgtataa gtttcgagta aagcaaatta tcttataata
     2821 atatgttaaa ataataataa taaaatgcaa agattatatt aactcaattg ttgtataatt
     2881 tatatagagt atctcagttt agctggaaaa taaggtatgg taaaccttac catgctattt
     2941 tgcataacaa aattctatta cgggaggtat atgagtcata tgacaattat gataaaaaat
     3001 acaaacagta attttgaaag agaaccgcta ttggctcctt ttggttttaa aggaggttat
     3061 cttagtgaat tgtggcaggt tgtagccctt cttgaaagtg aatcgggaaa aaggggtacg
     3121 ggtctaggag tccagagtgt tctttggtct gatgcggcag tctttgcagc aaactcacag
     3181 gcagcgggta acagtgccat gtttcttatg actgaatttg ctttacaaaa agccagagat
     3241 ttaagtttca aaactcccat agaacttcta gaacagcttt tgcctttgac ttatgaatat
     3301 ggcagaaaaa taacaaataa cgacaaactg cgaatgacat ttgcattaaa ttcacttgta
     3361 gccgttgata atgcggcctg gatagtttat tgcaaagaaa aaggcataag caattttgat
     3421 gatatgattc ctgaagaagt taagcctgct ttgtctttca agcatgataa actagcttca
     3481 ataccattaa ttacatatgg ggtatcaatg gatggcataa aaaagacagt agatgatggg
     3541 tatttcttcc ttaaaattaa aattggatct gaccctgaaa gagacggaaa tttaaataaa
     3601 atgctggagt gggataaaca gcgacttacc gaaattcata atgctgtgaa agatataaaa
     3661 ataccttata ctgaaaatgg gaggattccc tattatttag atgcaaatgg ccggtatgac
     3721 agcagggaca gacttatgaa gctgcttgac tatgcagata aaataggtgc tcttgagagg
     3781 attattcttc ttgaagaacc ttttccagaa gaatacaaaa ttgatgtaag tgatgtacct
     3841 gtaagattgg cagcagatga aagtgcccat tcagatatgg atgcaattga aagaattgac
     3901 ctgggatata gtgcaattgc actaaagcct attgcaaaaa ctatgagtat gagttttaaa
     3961 attgctaaaa ttgctcatga gagaggagta ccatgctttt gcgctgattt aacagttaat
     4021 cctgttatgg tagactggaa taaaaacgta gctgccaggc tctcaccatt acctggtatg
     4081 aaaattgggg tgcttgaaag caatggacac cagaattatt gcaactggaa gaaaatgaag
     4141 gaataccatc catgttatgg tgctgaatgg atagaaacag tagatggaat ttttagcctt
     4201 gatgaggagt tctacctgga aagtggtgga atatataaag agagtaaaca ttatgagtcg
     4261 ttggtgaaat gaaaaatatg tttaaaaatt cgttaagcag tgaaaattta aaatctatat
     4321 tagaagcatt tagcataaag gattttgatg aagttcttcc tatagcaaat ggaaccgcca
     4381 ataagaatta tcttgtcgtg gcttcaccaa gcttggtaat acgggaaaga agttccaaat
     4441 attcatctga agaacaaatg ctttttgaag aagagtacct gaaccatgtt ttctcaaaag
     4501 gcatacctgt tcccgtaccg ttaaagactt taaaccacct atgctggtat agctgccata
     4561 atagggttta ccaggtatat ccttttatag gcagcaaccc gggtgataat agggactaca
     4621 atgtaggtga tggcaaaggt aattgcgcag atagcattga aggtgacggt gaaaatgatt
     4681 ataatggtaa aaatgatgtt gaggaagccg gaggttttct tggaagactt cattcagctg
     4741 taaatggatt cacaccacag actctgagga atttgcctcg ctatgacgac ccttccataa
     4801 ttatacatga ggtttcgcga ataatagaaa agagcatcaa tactataaca gtggaagaaa
     4861 ggaaaactct tgaatatata ttggagcagg caaagcttgt aaacatgcat ataaacgacg
     4921 aagtttatta tagcttgccc aaattactga ttcatgggga ttaccaccct gccaatgtta
     4981 agtacatgaa tggtaaaata agtgggttat ttgactttga ctgggtaagc cttcaacctc
     5041 gtatacgcga tatcacagat ggcataatat atttttcttc aaaaagaaat gaaaacttaa
     5101 caggaaataa tatattttca ttagctacag gatatatctt tgatttagaa ggaatgagac
     5161 tgtttataaa aggttattct caaaatgtca gccttcctgt tttagaggag gagatcaaaa
     5221 atataccgta ttttatccga gccaggctga tgcatagtag agtgcaggca cttaaaaaaa
     5281 tacctgaagc actttctatt aaaatgctga ctgaaggtat gaagcattta ttgtcatgga
     5341 tagatgaaaa ccaaaacagg cttatagaaa atttgatgga ttgaataagc aaataaaact
     5401 ggggcgcatt ttctattccg ccaggttagg gtgcatttac tttatctgaa gaaaatattg
     5461 gtaaggggca taagtggagg ctctaggcac cgtaacgata gcccctggta aatatttctt
     5521 cgaatttgat aaatgcacct gccaggttat tctcccttga atacaaaggt acaatagtat
     5581 atactattat ttgaagtcta taattcaagt ggtgtcagat acaacagaag ggagaatata
     5641 tgcacatttt actaccggtt gatgtaatgt ggatttcgaa aatcaaatat gatcacaagg
     5701 ttcttgaaaa acatacccac caattctacc acatgttttt tataagaaaa ggcgaatgta
     5761 taatgcatgt tgctgggaaa gagtatactg cagatgaaaa tgaagtgtat ttctgccctc
     5821 ccgaaatagt tcatgggatg atagcgcagg gaaatatccc attaagtaca atagaagtta
     5881 aatttaccgt tcttgacaag aattttgctg aaaagttgtc agaactagat tatagggtaa
     5941 agtgcaaacc acataatatg tacttgaagt tagaggaact tgtaatggaa ggcttgcata
     6001 aaccagtgct ttacaaggaa attgtaaata cagggtttac cagagtattg ctggagttga
     6061 tgagaaatga agaaaatcca ttttcaataa acattgatcg ggtaaaaaac agtatgaaca
     6121 atgaaattaa ggataaagag ggaagtagtt ttgttcttgg gaaagtcctt gactatatga
     6181 accggatgta tgctgaagac ataactcttc aaaacttggc ggatgtagga gctgtaagtt
     6241 ctgcacatct gaacaagcta tttagaagta cttttaatat ttctcctatg cagtatataa
     6301 ataacatgcg tctcgataaa gcaaaagaac ttatgatgta ttctgatttt aacatttctc
     6361 agatatctga gctggtaggc ttcgaaagta tccattattt cagcaggtat ttcaagaaaa
     6421 aagaaggcat ttctcccagc gaatttagaa atggcgttaa agataatatt tatgtctatt
     6481 tgtgatttcc gttcaaaatg ttcaaaatat agctaaaaaa gtgtaaagaa tttatattca
     6541 atgtcttata taatcaatac taggagctgc tttgactttt atcttgctta aatttggcaa
     6601 aaaactatta gcagtcttgc atgatatatg tattggcagt tatacatgat gtttgtattc
     6661 aaaattaaat gaaagggaat ggtgagatat gagaagcttg aaagttggtc ttattgggtg
     6721 cggaggtttt gctaaaggta tgcatattcc catacttaag agtaatccca aatatacaat
     6781 tcatgcggcc atggatgtat atgaagatgc tgcaaaagaa gtagcagaga gtactggagc
     6841 tgcttactgg acaaaggatg tagataggtt gctttctgac agggaaatag atgtagtatt
     6901 tattacaaca cgtcatgact cacatgcaga gcttagtata aaggctgcga acgcaggtaa
     6961 gcatgttatg tgtgaaaaac ccatgggcct taaccgtgac cagtgtaaag cagtcgttga
     7021 agctgtaaaa aggagtaaag taaagtatac cgttggctac aatagaggga tggcacctat
     7081 ggtaacaaaa gccagagatc ttattgataa tgagctgcac gggagtaaaa agatgatata
     7141 tcatcgtata caggctccat tccgggaatc gcactggaca catctgcctg aaataggtgg
     7201 aggacgcttt gttggagaag gatgccatat ttttgacctg ttatgtgaaa tagtacaggc
     7261 tcctcctgtt actgtttttg cttcaggggg gacattcctc aaccctgaga aagtaaaaat
     7321 acctgatagt ggtgttgtta caattacatt tgctgacggt tctataggga cgacactaat
     7381 aaacagtgcg ggttgtcctg attttcctaa agaagccaca gagatatact gtgatgggaa
     7441 agctatattt ataaacgatt ttactaaaat ggagtattat gggttcgaag ggcataaaaa
     7501 gacttatatg gaatttgaca aagtagacaa aggccatgca atagaattgg atatgctggc
     7561 agatgctata gtatatgata cagaaagtcc taatggcttg gtgaaggcat caagagccgc
     7621 agttataagc tttatggtaa atgaatcgat aaaaacagga gcacctgttc aaattaatga
     7681 gaaggattat atatggtgaa gaaagggaga atcagcgata tgattagaat tggagttgta
     7741 aatattgatg tgtcacaccc gaagacattt tcggaatatt tgaagaaagg gaatcgtgcc
     7801 cgttatgtgg cagtttataa cgatggcttc cgtggtgatg acgaggttca gggatttatt
     7861 aaaaactata atctggaaaa acgctgcgag tctgtagaag aactggcaga tatggttgat
     7921 gtaggtttta tacagggatg taattgggat aaacatataa aatatatcaa accttttata
     7981 gaacgcaaaa agcctgtatt tatagacaag cccatagtag gctcccttgc agactgcagg
     8041 aaaatagagc agctggttgc agaaggcgct gttattttag gtagttcttc agtcagatat
     8101 gcagaagaag tagttaaatt tgcaagcacc agtgaagaag agagaggtaa gattcttaac
     8161 atttttggga cttccggtgt agatgagttc aactatagca tacatattgt agaagccatc
     8221 ggaggaatag caggtacagg tgcagtttca tgtacatttg caggcagaag tgaagttgat
     8281 gggaaaatgt gtgaaacatt tttcgtgaaa ttcgctaatg gaataacagc tgtatacaat
     8341 gctttccagg ggctgtggca gccttttgaa atggtgatta tgactacaaa aggaacatat
     8401 cagttccgta ttgatacagg aaaaatttat ggtgctttac tcgatagaat atgtgattat
     8461 atggaaaccg gaaacaatac acttgctcca attgaagctt tgacggaatc tgtaaaaatt
     8521 atgcttgcag ggcgaatttc aagagaaaag agcggagtag aggtaaaact attagatatt
     8581 cctgaagatg atcctggata tgatggagat agatttgaga aggggtatgc ttcaaaagct
     8641 gcaaaaactt atctttaata ttacttcaat gactaagagc agcttagatt ttgcagcaaa
     8701 atcatttttt agatgttgtc tacaatgcaa aaacattata taatgatagc gaaagccagt
     8761 tacatcatga agacagaaag ccggtatttg cagtttaaaa agaggctaat aaattaatat
     8821 gggggaatca ttaatatgat gtattttgct gttgcgctag gtattttgtt ctgctctttt
     8881 tgggttgcac agttaatatt tgtgtacctg caccttcatt ggactaatac tattattgag
     8941 tcaaatgatg gagacgctga tgtacctgta actgtaatcc atccaatcaa agacttggac
     9001 ttcgagtttg agaagaactt tgagagttgg atgattcaaa attatagggg aaaggtacag
     9061 catattttca gttttcagga accggatgat cctgccatta aggttgtaaa aaaacttaaa
     9121 gagcgataca acgacaggga tatagaaatt attgtaaacc ctgttatgga agggttgaac
     9181 ggtaaaagct ccaacatggt taatggtatg aagctggcaa agtatgatat agtaatattt
     9241 atagacagtg atataagaat taaacctgat tttattgcta aaatgacaag accgctggct
     9301 gatgaaaaag ttggaatcac aacttgcgga cagataaata ttggaggaag tgatttttgg
     9361 acaaggtttt ttacttttgt acagaatagt gaaactgatt tcatatgggc atttttaaca
     9421 aagatggggt ttgatgtagg agctacagga gctgcttttg gtatgagaaa aaaacttctt
     9481 caggaaattg gtggacttga agcttttggc ggctctttgc tggaagacct tcatcttggg
     9541 aacactcttt ataaaatggg atataagctt gttcttggcc catttcttga atgccatgtg
     9601 gaccgtatag caagggagaa gtcgtttaac tatgcaaaaa gaatagcggt agggataaag
     9661 acccatatag cagtagacct gcctgcattt ataataattc tttcatggta ttgggtatta
     9721 tttataattg ctgtactaac acaaaatgag aagctcctgt acctttcatt attctatatg
     9781 gcctttagga cggtacatgg attatttcac aggaaagtta ccatgaacaa ggtgttgtca
     9841 gtggacctgc ctatggctct gttttttgat ttatttggta ctttttatct attatatgca
     9901 tttaacaggc cttatgttac atggagaggt atagagtata aagtaaaaaa aggcggatat
     9961 attgaaaacg taagctttaa tgaagcagcg attcttgatg aagattttat caatgagcaa
    10021 tttattgatg ttgatgttgg tgatgaagta gaagatgtac aaaaataggt tgcagtttaa
    10081 attatattat tttatgagta tgtcatatgc ccaggttgga gtatcccagc ccgggcataa
    10141 taattcgggc gtaataatta ttgatgaaac tggagaacca actggtatag tgttctagac
    10201 agtggaatat tcattatggt agatacatga gctgtactcc taagccaatt ctttcctgaa
    10261 aattctatat gttttatagt gttgaccacc tatgctttct atatcattac gcatattaac
    10321 attttcttca cctatagttg agccttcacc ccaggtatat ccttttttca ctccattatt
    10381 aaaggcataa tgataaactg caaaggaaac acctttcttt ctgtattcag gaattacaaa
    10441 cattataaat acgcgggcac cattaattct tttcttataa tatgcatact ttagtatgga
    10501 aatagggttt atacgcccgt taaggtgaat cagaagctga ttataatcgg gtaaagcaac
    10561 tgcaaatcct attgccctgt tgttattggt tcttgcaatt gcaaccaggt caggatcaac
    10621 gtatgatata tagcttttag ctattgacct gacttcatcc atatcgggag ctgccaggtc
    10681 cgaccattcg gcaggaatag caagatcaag gatgtatttt atatcttcaa gttccttatc
    10741 tagattcttt aggttcaaag aatcaaccct gaaatcatat tttcttttag cataatccag
    10801 tacttttgat gggtttttgc taaagatctt atcagggtcc aggtaatagg caaagacatc
    10861 ataatctttt tccaacccat actcttcaaa aaggcggata taatactctg gattataaga
    10921 attcatgagc actgggggac gatcaaaaca atctatgaga aggcccttgc tttcatccag
    10981 atctgcacct tcagtggaga ccggtcctct tacaagattt ataccctttt ccttaaacca
    11041 ggaaaatgct gtatcaaata aggattttgc aacttcaata ttatctatgc attcaaatag
    11101 agaaaaatac cccatctgtg cattcttttt ttcgttaaga agcctgttta tccctgcata
    11161 tatccttccc acaggttttc cttccagcat ggcaataaac aattcacact ctttttcccg
    11221 tttatcggga ttaatcagcg gagccagatt ttttttcata tctctgataa gaggaggaac
    11281 ccagttgctg tttcctttat atattcccca gggaagcatt ataaattttt ttaagtcagt
    11341 catggacctg accggaataa cttgaatatc catataattt ccgtccttat ttgaaattgt
    11401 tatttgaaat tattgaaatt aaataacatg tttattatat taaataactg ttccattatc
    11461 aacacctcat atcaaacaaa acactgggtg taggggtgca tttaaatttt ttggagaaat
    11521 agattccata ggttatcgtt acggtgctta gaacttttaa atgcacctgc actaatgctt
    11581 cttccaataa tttcttcact caaattatat gcacccctta gtgcatttta cctttgactt
    11641 tatctgtcat ttgaattaaa attgtattaa tgttaaacat aatctttgaa atgtgcaata
    11701 tatctaatgt tacattaaga attttttgat ggaggctggg tttttatgaa tactaaactg
    11761 agagatcctc ttatagataa actatttgag gctgttttac tactgaaaga tgcagaagag
    11821 tgttacaagt tttttgagga tatatgcaca atagcagaaa tcaaggctct tgctcagaga
    11881 ctggaggtag caaggctttt aagagaaaag aaaacataca atgatatcag tgatttaacc
    11941 ggtgcaagta cggcaactat aagcagagtg aatagggcgt taaattatgg cgcagatgga
    12001 tataatatga tccttgacag gctggatagc aagaactgaa gagtatgttg cagagaagca
    12061 taccatgcag tgatggatat agtatagtag tggatatagt gcggtgaagt attccattca
    12121 acaacggata tactgaagtg tttgataggg aggaaactaa ttatgttgaa gtttatagaa
    12181 aagatgtttg gaaattacag tgaaagagaa attaaacggt taatgccttt ggttgatgct
    12241 atagaagaac tggaacctga catagaaaag ctcagtgatg agaatctaaa aggaaaaact
    12301 gaggaattta aagaaaggct gtcaaacgga gctactcttg atgatatact gcctgaagct
    12361 tttgctgttg taagagaaac atcaaaacgt gttttaggta tgaggcattt tagtgttcag
    12421 ctattaggtg gcattgtcct tcatcagggg cgtatttctg aaatgaaaac cggtgaaggt
    12481 aaaacgctgg tggccacatt gccggtatat cttaacgctc ttgaaggaaa aggagttcat
    12541 gttgttactg taaatgacta ccttgcaaaa agagacagtg agtggatggg taaaatatat
    12601 aatttccttg gcttaagtgt tggactgata gttcatggac ttgataacaa ccagagaagg
    12661 aaggcgtatg ccagcgatat tacctatggt acaaacaatg agtttgggtt cgattacctt
    12721 agggataaca tggttattta taaagaagat atggttcaaa gggatttaac ctatgcaatt
    12781 gttgacgaag tggacagcat attaattgac gaagcccgta cccctctcat aatttcagga
    12841 gcaggagaca agtcaactga tttatacagc aaagccaatt cttttgttaa caggcttaag
    12901 cctgttgtat ttactgaaac agatgacaaa gagtttatgg atgatgttga tgctgactat
    12961 attgtggatg aaaaggccaa ctcggttact ttaactgaaa agggcataaa aaaagctgaa
    13021 agttattttg gactggaaaa cctttccgac cctgataata tgactatatc gcaccacata
    13081 aatcaagctt taaaagcaag aagtcttatg aagagggaca aagactatat agttaaagat
    13141 ggagaagtaa ttattgtaga tgaattcacc gggcggctta tgtacggcag aaggtacagt
    13201 gacggactgc atcaggctat tgaggcaaaa gagggtgtaa aggtagaaag agagagtaag
    13261 actcttgcta caattacatt tcagaattac ttcagaatgt acaaaaaact ggctggaatg
    13321 acaggtacag ctcttacgga agaagaagaa ttccgcacaa tatacaaact ggatgttata
    13381 gaaattccaa caaacaaacc aatgataagg gcagataatt ctgatgttgt atataaaaca
    13441 gaaaagggta aatttaatgc tgttattgat gaaatagcag aatgcaataa aagaggacag
    13501 ccggttctga ttggcactat atccattgaa aaatcagagt tgttaagtgc aatgcttaca
    13561 agacgtggta taactcacaa cgtgctgaat gcaaaacatc atgaaaagga agcggaaata
    13621 gtttcccagg cggggaaata cagtgctgtc actatcgcaa caaatatggc cggtcgtggt
    13681 acagatattg tgctaggagg taatgctgaa tttcttgcta aaatggaaat gagaaaacaa
    13741 gggtatcctt ccgatgtaat tgttgaggct gtaggattta acgaaaccga tgatgaggtt
    13801 attctggaat caaggaaaac tttcagagaa ttgtatgaac attttaagaa tcaaacagac
    13861 ggtgagaagg aaaaggttat tgctgcagga ggattgcata ttataggaac agaaagacat
    13921 gagtccaggc gaattgataa tcagctgaga gggcgttccg gccgccaggg agacccggga
    13981 tcttcaagat tctatatatc tcttgaggat gatctgatga gactgtttgg atctgacagg
    14041 ctttcaggaa ttgttgatgc tcttggacta gaagaaaatc agcctataga gcatagaatg
    14101 ctgtcaaatg ctatagaaag tgcccagaga agagtagaag gcaagaactt cgatataaga
    14161 aaacatgtac tgcaatacga tgatgtaatg aacaaacaaa gagaagtaat ttatggtcag
    14221 agaagaaatg ttctggatgg agagaactta aaagatagtt ttataaaaat gatagaaaat
    14281 atatcggaac aaattgttgc tgcatattgc agtgaaagtc cgcaccctga tcattgggac
    14341 ctggacggaa ttatttcata tgtagaaaac atattgttgc cccatggtac ctttaagatt
    14401 caagatgagg atatggagtt tcttacaaag gacagcttaa aagaaaaaat tcttgagatg
    14461 acaatggaaa cttatgaggc cagggagaag gaacttaatt ctgaattaat gagagaactg
    14521 gaacgggttg ttttactgcg tgtagtagat gaaaaatgga tggaccatat agatgcaatg
    14581 gatcagctta gatatggtat tggtctccgt gcttacggac agagagatcc tgtaattgaa
    14641 tacaaatttg aaggccatga aatgtttgaa gaaatgatta gaaacattca ggaagatgca
    14701 gttaaaattg ttcttaagtc ccatattgat acagggcgtg cacttgaaag acggaaagtg
    14761 gctgagccag ttaatgcagt ccataatgat cagccgggcg gcccagtagt gagatctcaa
    14821 aaagcagggc gcaatgaccc atgcccgtgt ggaagcggta aaaagtacaa gaaatgctgc
    14881 ggttcaaatt aggaagtaga caggtgctgt ggcacaaatt agaaagcaaa caagtgctgc
    14941 ggtttatggt ttgatagtgg aacgaatagg tgctgcagtt aaacttaaag agaatgtgga
    15001 ggttgtaaac agaaatctat gacttatgat gaagctctaa aatatataca tggaacacat
    15061 aaatttggag tgaagcttgg actggataat gtggcaaagt tgctggagct gctggataac
    15121 ccacaccaaa aactaaaata tgttcatgtt gcaggtacca acggcaaagg ttctactgtt
    15181 gcttttataa gcagtatact tattgaagcc ggttataaag ttggtattta cacttctcca
    15241 tatattgagc atttcacaga aagaataagc attaatcata aagaaatatc tgaaggggat
    15301 ttggcaagga ttaccgagta tataaaggga aaagtacaag aaatggtaag tatggggtat
    15361 aatcacccga cagaatttga aatagtaaca gcaatcgctt ttcaatatta ttatgaaaac
    15421 aaatgtgata ttgttgtact tgaggtgggc cttggaggca ggtatgattc tacaaacata
    15481 atcgggactc ctcttgtatc agttataact tctattagtt atgaccacat gagtattctt
    15541 ggaaataccc tatctaagat agcttttgaa aaggcaggaa ttattaaaaa agatggagat
    15601 gtagttctct atcctcagtc tcaagaagtt gaagaagtta ttgcaaaaac ttgtgaagaa
    15661 atgcattcga cacttcgtaa agctgatttc agtaaaatta tatctcttga atacggagcg
    15721 gatgggcagt attttaattg tggactatat gagggattaa aaacaaaact tttgggagaa
    15781 caccagttaa agaatgctgc agttgcaatt actgcctgtg aagtaataat ggacaaggaa
    15841 tgtaaaatta ctgaaaatac cggatgtaaa attactgtag atgctattag aaatggtatt
    15901 aaaaaggcaa catggccagg gagaatggaa atcgtatcaa aacagccttt gtttcttatt
    15961 gatggcgccc ataatgaaga tggagcaaga gttatggttg atacactttt aaaatatttc
    16021 ccgggtaaaa ggaaagtttt cataatggga gtactaaaag ataaagaata tgagaaaatt
    16081 gttgaaattg ttgcccccat tgcaaatcag tttataaccg taacaccttt gagtgataga
    16141 gctcttcctt ctgatgattt ggcggaaact ataaagcgct attgtaaaaa tgttataata
    16201 tgtgataaaa tagaaaaagg tattgaaaca tctcttcgga tatctgacag taatgctatt
    16261 atatgtgctt tcggatcctt atattatatt ggtgaagtga ggcggtactt tgaactaatt
    16321 aaattaacta tataaactat atgct
//
LOCUS       DAOSDD010000089        16319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1439_length_16319_cov_4.796606, whole genome shotgun sequence.
ACCESSION   DAOSDD010000089 DAOSDD010000000
VERSION     DAOSDD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_16319_cov_4.796606"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..564)
                     /locus_tag="PK733_09395"
     CDS             complement(268..564)
                     /locus_tag="PK733_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10793.1"
                     /translation="MAAIYSYYPKQGKQPLKPETDSFTGTINSSKNNETPYQNIDNRS
                     LNNSSENIENHNTELEYNTGMLKKIIEFKSEDILQGIIYSEILGKPKALRQKKR"
     gene            complement(580..1572)
                     /gene="floA"
                     /locus_tag="PK733_09400"
     CDS             complement(580..1572)
                     /gene="floA"
                     /locus_tag="PK733_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254616.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOJ10794.1"
                     /translation="MPDILSFILIVGVVIFLISSFFSLVPVGLWISAYAAGVKVGIFT
                     LVGMRLRRVIPSRIVNPMIKAVKAGLPASINKLEAHYLAGGNVDKVVNALIAAQRANI
                     PLEFERAAAIDLAGRDVLEAVQMSVNPKVIETPIIAAIAKDGIELKAKARVTVRANID
                     RLVGGAGEQTIIARVGEGIVTTVGSANSHKEVLENPDLISKTVLEKGLDSGTAFEILS
                     IDIADIDVGRNIGAQLQTDQAEADKRIAQAKAEERRAMAVAMEQEMKAQVQEMRAKVV
                     EAEAEVPKALSEALRQGKLGVMDYYNMQNIMSDTQMRSSISSAAKPESGYQDKK"
     gene            complement(1671..2189)
                     /locus_tag="PK733_09405"
     CDS             complement(1671..2189)
                     /locus_tag="PK733_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ10795.1"
                     /translation="MWLYELFGFVYRMTWFPALCFIFGFAFITFELFHPGFGAPGISG
                     IILLILGIMFTARSFLDAIVMVILVLVIIGIILLLVIRSASKGRLAKKLILSDSSNVE
                     SGFAGRENLESFMDKTGTATTILRPSGTGDFDGIKLDIVTEGEFIPKGTKIKVVKVEG
                     IRVVVKRTDDIS"
     gene            2513..3382
                     /locus_tag="PK733_09410"
     CDS             2513..3382
                     /locus_tag="PK733_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10796.1"
                     /translation="MKRQLKCTYFKKTGYPQLISERSCILLTFIIYLLPIFLSGCGAC
                     KKVTGHIKESISYDEKIIIFERSPEIEISGDAGNIEVFNHEEKSVKFEILKKIKRVYT
                     SQEELESDFKKFNITTEYKADKILLKFKYDTNKLNPENQSIDIRMYIPKNIKSINFKL
                     SSGKIKIYDDLNCNIFADLNMASMDIKKMNGILNFKGEMSNIKISCGTIKNGSIVKVG
                     TGNIDIKSEYEEGGYYEFETNTGNIDLSISSNFNISFESTGFVQMNEFKMGEFPTKIK
                     VKSDIGRICINKY"
     gene            3480..4781
                     /locus_tag="PK733_09415"
     CDS             3480..4781
                     /locus_tag="PK733_09415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ10797.1"
                     /translation="MWRNKQIIFSLILLVIMLIGIKLTNILKSTDKNTETSESLRTQE
                     SDNEVLNAQVQLSKESEQQNSSNTGSALLQKHTENISDSTNNPVSRQLRENHENNKIL
                     KEPVRIVAVGDIMLGRGVEYHLNRLGKDYTYPFQYAVDILRSGDMVFGDLEAPLTESN
                     HGLDPSGKIVLKSSEKAAEGLKYAGFNILNLANNHIMDYYEKGLLDTISVLDKHEIAY
                     SGAGKTIDEARKPAIIEKKGVKIGVISYTDMAELIFKGNPMISFAAEKEKSGVAPRKY
                     ELIKEDIESVRENVDILIVSLHWGVEDSFHATEEQREFARKLIDDGADIILGHHPHRF
                     QGVEIYNDKPIVYSLGNFIFDQNDPNNQESFIVNMEITDDKLSSLELIPIRTIEKTHV
                     IIPKGEEPFEMLKRELELCGRLDSKCAIVDNVIKFNIKCPE"
     gene            4983..5414
                     /gene="mraZ"
                     /locus_tag="PK733_09420"
     CDS             4983..5414
                     /gene="mraZ"
                     /locus_tag="PK733_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ10798.1"
                     /translation="MFYGEYQHSLDSKGRIIMPSKFREELGDKFILTKGLDECLFVYS
                     SQEWSNLESKLKALPFTDKDVRAFVRFFFAGATECELDKQGRILVPQNLREYAKLEKD
                     LYVIGVSTRVEIWDKGKWEKYCSEDNISPDKIAEKMAMLGI"
     gene            5443..6384
                     /gene="rsmH"
                     /locus_tag="PK733_09425"
     CDS             5443..6384
                     /gene="rsmH"
                     /locus_tag="PK733_09425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ10799.1"
                     /translation="MEFYHLPVLLDECIKMLSIKPDGTYLDGTIGGAGHSSEILKRLG
                     KDGTLLGLDRDEYALESSRKRLDTIETKAKVILVNSNFMYIKKVCSENGIDEADGVLL
                     DLGVSSHQFDEAERGFSYQKDAPLDMRMDRTQPLTAEVVVNEYSESDLYRIIRDYGEE
                     KWASRIASFIINARAERRVSTTLELVEIIKAAIPAAARREGPHPAKRTFQAIRIEVNN
                     ELGILRDTIDDAVSVLKTGGRLCIISFHSLEDRIVKTEFQRLVNPCSCPVDFPICVCG
                     KRPVAILTVKKPILPSEREICSNPRARSAKLRVIEKL"
     gene            6717..7205
                     /locus_tag="PK733_09430"
     CDS             6717..7205
                     /locus_tag="PK733_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOJ10800.1"
                     /translation="MVQKNNKYVYGTAAEKIQYDVYEKNKVLKEKKQYKANNAIKLKM
                     VASILLAFLIGFAVMYRYALIADMNYKISNMERQYEKLKNENSRLKVAIENETNLSRI
                     SEIAQNELGMQKPDKYQIVHIRVPKTSYTVISDAYKNTTAKENLFVELVRKGVAIKKL
                     FN"
     gene            7311..9488
                     /locus_tag="PK733_09435"
     CDS             7311..9488
                     /locus_tag="PK733_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ10801.1"
                     /translation="MAGPGLQAKKRLLIVLIGFFLIFVILLGHVGYIQIVQGQILKSM
                     AYEQQTRGRVITPKRGTIYDRNGKQLAVSASVDTIAVNPKDVAKSGISKEIIAKKLSE
                     ILEIDEEKIIAKLEKNTWYEEIKEKVDKNIGDEVRKWIKDEKIEGVRVDEDSKRYYPH
                     RNLASHIIGFIGKDQGLDGIEAIMEKYLKGVPGKILSEMDAGGRTMPYTPERRVDTQD
                     GLDVVLTIDETIQYLAEKAIEKAIDDNKVLNGAVVIVMAPRTGDILALVSKPDYDLNN
                     PFAAPPGVDPEKWKGYTNEDVTKLQETVWRNKAVSDTYEPGSTFKAITAAAALEEGVI
                     KPDSPVNDFPVKVGGWNIGCWRAAPHGQETFREGVYNSCNPVFVRVAQDLGIKTFYDY
                     VRGFGFFEPTGVELPGESNSIFHKPPVEIDMAVASFGQRFQITPIQLITAYCAIANGG
                     YLMKPRLIKELRDSQGNIVKKFEPEVVRQVISSETSDTLRDILEGVVSDGTGRNAYIK
                     GYRVAGKTGTSETTETKSEGRYIASFSSFAPADNPVVNVLIVLDHPAGDSYMGGVIAA
                     PVAQKLLDDVLNYLGVEKRYTEKDKETIRLPVYVPEVRNISLNDAKSTLAKYGLEYRV
                     EGDGNNKDAIILEQMPKPGASIPEKSVVILYTYKPDTEVMAKVPNLLNKTVYEATQAL
                     KNAGLNIKVNGDGTAVKQEIVPGSEVPVGKVIEVDFRHLDNVE"
     gene            9555..11042
                     /locus_tag="PK733_09440"
     CDS             9555..11042
                     /locus_tag="PK733_09440"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOJ10802.1"
                     /translation="MKLHELIEGLNPNVISGDCDLDIRDIVYDSRKAGKNSLFVCIDG
                     SVVDGHAFIPEAIENGAGALLVEKEVNVPPNITVIKVKDTRYGLAFVSDKIYDHPSRK
                     LNLIGITGTKGKTTTTYMIKHILEDAGLKTGLIGTIQNMIGDEVLYAERTTPESYDLQ
                     ALFSEMCEKDVKSVVMEVSSQGLQLNRVSCSHFEIGVFTNLYKDHISPTEHKDMEEYL
                     NAKIKLFKMCEKGLVNIDSPYAAKVLAEAECPIYTFGIENDCDIRAYDIIKHPHSVEF
                     QVCSPWVNGHILVNIPGKFSVYNALAAIGVCCLSGVSFDNIKNGLKSISVPGRAETIH
                     TGRNFTVIVDYAHTADSLENILSTVKDFSKGRVVSIFGCGGNKDKGRRFGMGEVSGKI
                     ADFTVITSDNPRSEDPAAIINDIETGIKKTEGKYIKIVDRREAIRYAIESAKPEDIIV
                     IAGKGHETYQIFKDKTIHFDDREVVREILSETASEKSEEIVLQKE"
     gene            11102..12517
                     /gene="murF"
                     /locus_tag="PK733_09445"
     CDS             11102..12517
                     /gene="murF"
                     /locus_tag="PK733_09445"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOJ10803.1"
                     /translation="MKILRCGEIVEATEGILLYGSSEQEIMRISTDSRSVQPGSLFVP
                     IKGERFDGHNFIKDALAAGAAGTLTQYDLQELKCCPEHEGSFDGKLIIKVNDTLKALR
                     DIAAYYRQMFRIPFTGITGSVGKTSTKDMIACVLEQEYNVLKTMGNFNNEIGVPLTIF
                     NLNDSYEAAVVEMGMSGFGEISRLTSIVKPKIAVITNIGMSHMEKLGSRQNILKAKME
                     IFEGLPRDGLVVMNGDDTFLYGLKGFLNFRTLYYGMDEGLDYQAYNVQSAGETGIYFD
                     INVKNVEYKVHVPVPGIHNVYNALAAIAVGVESGISMKNIIQGIRNFIPGKMRLNIVK
                     NNGIKVINDAYNASPQSIDAALKVLIDLEDVNRRIAVLGDVLELGEWARQAHLDIGKL
                     AFNMGIDYIITVGENGRYIAEGALESGASVKNVNHFSDNKEVTRFLEDFITDGDAILV
                     KGSRGMKMEEIVEQLIRGENA"
     gene            12514..13509
                     /gene="mraY"
                     /locus_tag="PK733_09450"
     CDS             12514..13509
                     /gene="mraY"
                     /locus_tag="PK733_09450"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ10804.1"
                     /translation="MNEYFKVSIQVVAFILTFILSLIAGPIIIPMLRRLKFGQTVRDD
                     GPSTHIKKTGTPTIGGVIFIIPITLVSVYFSAKYPDIIPLIIVTLGFGAVGFIDDFLK
                     VVKKSKDGLFWKQKMFALLVIAVAFTMYGINKGILNTEMIVPFMGMDYTVALPMWFFI
                     PFTIFVLLAITNAVNLTDGVDGLATGVTLIVVVLFTIIAMTRSDWDYLKIFSSTVAGG
                     CLGFLAFNAYPARVFMGDTGSLALGGAVGAISILMKMPWIILVAGFIYVIEALSVIIQ
                     VSYFKLKGKRVFRMAPIHHHFELLGWKETKVVSVFWVITFVSCIIGLLTLRLKFF"
     gene            13534..14640
                     /gene="ftsW"
                     /locus_tag="PK733_09455"
     CDS             13534..14640
                     /gene="ftsW"
                     /locus_tag="PK733_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOJ10805.1"
                     /translation="MKGRKPFDFWIFMTVLILLSIGIITVFSASAPFSYSNFRDIYYI
                     LKKQLLYGAIGLVAMFVAMNIDYRILAKYSSLLLIISIVMLIAVLIPGIGKEENGSTR
                     WIEFGSLRFQPSEIAKIAIILYFSSSLSKRKSPLNSFFKDLLPYLILIGVISGLLLLE
                     PHLSCTIIILIVAMIILFSAGAKIKHFMILFVPAASAFAALVAFVPYMNRRVLSFLDP
                     WKDMQGDGYHAVQSLYAIGSGGLFGRGLGKSMQKFLYLPYPHNDFIFSVLAEELGFLG
                     VLTVMVLFLMFTWRGIKVAMSAPDLFGSYTAVGITSLISVQTVLNIAVVSSAIPPTGV
                     SLPLFSYGGTSLVLIMGAIGILLSISRYTSYGRI"
     gene            14828..16087
                     /gene="murA"
                     /locus_tag="PK733_09460"
     CDS             14828..16087
                     /gene="murA"
                     /locus_tag="PK733_09460"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ10806.1"
                     /translation="MSKIIINGGRKLKGEISVEGSKNAILPILAATLLNSGRSVIRNC
                     PKLKDVEVTLRILMKLGCKVEVDKDTVMVDSSNITSTEIPVDLAAEMRSSIIFMGPML
                     ARCGRVVISFPGGCEIGPRPIDLHLKGLRSMGANILDVQGGFIYCNADHLKGTEIHLS
                     YPSVGATENIMLASVFAEGETYIHNAAKEPEITDLQNFLRAMGADVSGAGSGIICVKG
                     TAQKLKDVEYTIMPDRIVAGTYMVAAAITGGEVTLNNVMPEHLSAVISALSEAGCTIK
                     NDENHIYICGPDRNEAVDIIRTLPYPGFPTDMQAPFVSLLTVCRGTSIVVETVFENRY
                     KHVDELMRMGANITLEGRIAVIKGVDKLNGAVVYARDLRGAAALAIAGLAAKGETVVL
                     DIKHLDRGYENMEVKLAQLGALIRRQD"
     gene            16107..>16319
                     /locus_tag="PK733_09465"
     CDS             16107..>16319
                     /locus_tag="PK733_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10807.1"
                     /translation="MHQDKKYKINTNTGNLKYKDKSIRHIRKKLEYITILFLIVAGLI
                     VLIMSPIFNISNVYIYGNSHYSTEEIS"
BASE COUNT         5681 a         2432 c         3381 g         4825 t
ORIGIN      
        1 cgatttgtgg aaacaggaac agagacgaat atggatgtgg aaattaccaa aggcctcaac
       61 ccgggagaca aggttataga tagctaattg tgtggctggg aagggaaaaa cttagaggtg
      121 agaagtggag gtgagagtga gaaattgggc agacaaagca agtttcttgt tttattatta
      181 attctcacaa ctcacaactc acaactcaca acttatcctt aacttcaaac atctaatttc
      241 taacctctaa cctctaaact gttttgatta gcgttttttt tgccttagag ctttgggttt
      301 gcccaatatt tcagaataaa ttataccttg cagaatatct tcagatttaa attcaataat
      361 tttcttcaac attcctgtgt tatactctaa ttctgtatta tggttctcaa tattctcact
      421 tgaattattt aaactcctat tatcaatatt ctgatatggt gtttcattat ttttactgct
      481 attaattgta cctgtaaatg aatcagtttc aggttttaat ggttgctttc cctgctttgg
      541 atagtatgaa taaatagccg ccacctaatg tcaactcctt tattttttat cctggtagcc
      601 tgattcaggc tttgccgctg aagaaattga ggatctcatt tgagtatcag acataatgtt
      661 ttgcatatta taatagtcca taactcctaa tttcccctgc ctcagggctt ctgataaagc
      721 tttgggaacc tcagcctctg cttctacaac ctttgctctc atttcctgaa cctgagcttt
      781 catttcctgt tccatagcta cagccatagc tcttctttct tcggctttag cctgggcaat
      841 acgcttgtcg gcctcagcct ggtcagtttg caattgagcc ccgatatttc tgccaacatc
      901 tatatcggca atatcaatag aaagaatttc aaaagccgtt ccggagtcaa gacctttttc
      961 aagaactgtt ttagatataa ggtcagggtt ttccagtact tccttatgag agtttgctga
     1021 gccaacagtt gtaacaattc cttcaccaac acgggcaatt attgtctgtt cacctgcacc
     1081 cccaacaaga cggtcaatat ttgcgcggac agtaaccctt gctttggctt taagttcaat
     1141 accatccttg gcaattgcag caattatggg tgtctctata accttaggat ttacactcat
     1201 ctgaacagct tccagaacat cacggcctgc aagatcaata gctgcagccc tttcaaactc
     1261 aaggggaata ttagctcttt gtgcagcaat aagagcattt acaaccttat caacatttcc
     1321 accagcaaga taatgggctt caagcttatt tattgatgca ggcagaccag cctttactgc
     1381 tttaatcata ggattaacta tcctggaagg tattactctt cttaaacgca tacctacgag
     1441 ggtaaagata cctaccttca ctcctgctgc atatgcggaa atccagagcc ctacaggcac
     1501 taaggaaaaa aatgatgaaa taaggaaaat cacaactcca acaattaaaa taaagcttaa
     1561 tatatcaggc atttaaaaaa cccctttcaa aaattatata ttattgccat acacatatac
     1621 ataatgactt ttgcattaaa agtataggcg tattttaaag ttaaaagaac ctaactgata
     1681 tcatcagtcc tttttacaac tactcgtatg ccttcaactt ttacaacctt aatttttgta
     1741 cctttaggta taaattctcc ttctgtaaca atatcaagtt ttataccgtc gaagtcacct
     1801 gttcctgaag gacgtaagat agtagttgca gtgcctgtct tatccataaa tgattctaga
     1861 ttttctcttc ctgcaaaacc ggattctaca ttcgacgaat ccgacaggat cagcttttta
     1921 gccagacgtc cttttgatgc tgatcgtata accagtaaaa gtattatgcc aatgataact
     1981 aatacaagaa ttaccatcac aattgcatct aaaaagctac gagcggtaaa cattataccc
     2041 aaaataagga gtataatccc tgaaattccc ggagcaccaa aacccggatg aaaaagctca
     2101 aaagttataa atgcaaatcc aaaaataaag caaagtgcag gaaaccaagt cattctataa
     2161 acaaaaccga acaattcata caaccacatt aaaaatcacc ccctcataag agaaaaataa
     2221 atatagtaaa aaatatggga aattaatact gctttgtcaa gtttccaatg gaaatcgctt
     2281 tccaaattgg cgtagcagta ttaaatattt atttatacat taattatata tgattttcaa
     2341 aaataataaa acacctaaat gaaaattaaa caagaaattt tataagtggg aaatatttgt
     2401 agtgtaaaag ctttattgtt actatttaat tagaagatag aagatagaag atagaagata
     2461 gaagccagat attatgttcc ttaatttaac aattttaatg ggaggataaa taatgaaaag
     2521 gcaattaaaa tgcacatatt ttaaaaaaac tggatacccg cagttgatct cagagcggtc
     2581 ttgcatctta ttgactttta ttatttatct tttaccaatt tttttatcag gatgtggggc
     2641 ttgcaaaaag gttacaggcc atataaaaga aagtatttca tatgatgaaa aaataattat
     2701 ttttgaacga tctccggaaa tagaaatatc aggagatgca ggtaacatcg aggtatttaa
     2761 tcatgaagaa aaatctgtta agtttgagat cctcaaaaag ataaaaagag tatatacttc
     2821 acaagaagaa ttggaatcag attttaagaa atttaatatt actactgaat ataaagctga
     2881 taaaattttg ctcaaattta aatatgatac aaataaatta aaccctgaaa atcagagtat
     2941 agatattaga atgtatatcc ccaaaaacat aaagtcaata aattttaaac ttagttcagg
     3001 aaaaatcaag atatatgatg atctaaactg taatattttt gcagatttaa atatggcaag
     3061 catggatata aaaaagatga atggcatact taatttcaag ggtgagatga gtaatattaa
     3121 aatatcatgt ggaacgatta aaaatggttc tattgtaaag gtaggtactg ggaatattga
     3181 cattaaatca gaatatgaag aaggtgggta ttatgaattc gaaactaata ccgggaacat
     3241 agatttatca atatcctcaa attttaatat aagctttgaa agcacaggtt ttgttcaaat
     3301 gaatgaattt aaaatgggtg aatttccgac aaaaataaaa gtcaaaagcg atataggaag
     3361 aatatgcata aataagtatt aaaagctcta gaatttaatg tatatccttt gctatcaact
     3421 ataatcttta gcttttaata tttgtttatt tgtgatagta ttttaacggg gtgaattaaa
     3481 tgtggaggaa caaacagatt atattttcac ttatattatt agtcataatg ttaataggta
     3541 taaaattaac aaatatttta aaatctactg ataaaaatac tgagaccagc gaaagcttaa
     3601 gaactcaaga atcagataat gaggttttaa atgctcaggt gcagttaagt aaagaaagtg
     3661 aacagcaaaa ctcatcaaat acaggtagtg ctttactgca aaaacatact gaaaatatta
     3721 gtgattcaac taataatcca gttagtagac agcttcgcga aaatcacgaa aacaacaaga
     3781 tcctgaagga acccgtaaga atagtagctg tgggtgatat tatgcttgga agaggtgtgg
     3841 aatatcattt gaatagattg ggcaaagact atacatatcc tttccagtat gcggttgaca
     3901 ttttgcgttc aggtgatatg gtttttggtg atctggaagc tccgttgaca gaaagcaacc
     3961 atggccttga tccaagtggt aaaattgtgc ttaaaagtag tgagaaagca gcagaaggtt
     4021 tgaagtatgc agggtttaat attttaaatc tggccaacaa tcatattatg gattattacg
     4081 aaaaaggtct acttgatact atttccgtac tcgacaaaca tgagattgct tattcaggtg
     4141 caggtaaaac aattgatgaa gcccgtaaac ctgcaattat tgaaaaaaag ggagttaaaa
     4201 ttggagtaat atcctataca gatatggcag agttaatatt taaaggtaat ccaatgataa
     4261 gttttgctgc agaaaaagag aaaagtggag tcgccccaag aaaatatgaa ttgataaagg
     4321 aagatattga atctgtaagg gaaaatgttg atattttaat tgtatcactt cattggggtg
     4381 tggaagatag cttccatgca acggaagagc agagagagtt tgcacgcaag ttaatcgatg
     4441 acggtgctga tattattcta ggacatcatc ctcacagatt tcaaggtgta gaaatatata
     4501 atgataagcc tattgtatac agccttggga actttatttt tgaccaaaat gatcccaata
     4561 atcaggaatc atttatagta aatatggaaa ttacggatga taaattaagt tcacttgagt
     4621 tgatacctat aagaaccatt gaaaaaactc atgttattat accgaaaggt gaagagcctt
     4681 ttgaaatgct taaaagagag ctggaactgt gtggcaggct tgactctaaa tgtgctattg
     4741 tagataatgt tataaagttt aatattaagt gtccggaata gtattatgta aaacctgaat
     4801 aatgatgata aaaagtggac attccgttaa taagataaat gaaaacaacg gagtgtctat
     4861 tttttttgga aataattctt agaaaacact tgattatttt aacaatatgc aataaaataa
     4921 cttataggtg gagcgaagtg gtgagaagtg gttgaaagtg gtatagatat gaggtgaatt
     4981 aattgtttta tggtgaatat cagcattcac tggattcaaa aggtcgtata ataatgcctt
     5041 ctaaatttag agaagaactg ggagacaaat ttattcttac aaaaggcctg gatgagtgtc
     5101 tgtttgtgta ttcatcacaa gaatggagta accttgaatc aaaactgaaa gcccttccct
     5161 ttactgataa ggatgtaaga gcatttgtca ggtttttctt tgcaggtgct accgaatgcg
     5221 aattggacaa gcagggtaga atattggtac cgcagaatct gagggagtat gcaaaacttg
     5281 agaaagatct ttatgtaata ggtgtttcta ccagagtgga aatttgggat aagggcaaat
     5341 gggagaaata ctgcagtgaa gataacatca gcccggataa aatcgctgaa aaaatggcaa
     5401 tgcttggaat atgacattat tattagttta tgaggtgttg aattggagtt ttatcattta
     5461 ccggtattac tggatgaatg cattaaaatg ttaagtataa aacctgacgg aacgtattta
     5521 gatggtacaa taggcggtgc aggacattca tcggaaatcc tgaaaaggct tggtaaagat
     5581 ggtacgctac taggacttga tagggatgaa tatgctttgg aaagttcccg taagaggttg
     5641 gacacaattg aaactaaagc aaaggtaata ctggttaaca gcaatttcat gtatatcaaa
     5701 aaagtttgtt ctgaaaatgg aattgatgag gcagatggcg tacttctaga ccttggagta
     5761 tcatctcatc agttcgatga agctgaaaga ggtttcagct accagaaaga tgcacccctt
     5821 gatatgcgaa tggataggac tcaaccttta actgcagaag ttgtagtaaa tgaatatagt
     5881 gaaagcgatt tatacaggat tataagggac tatggtgaag aaaaatgggc atcaaggata
     5941 gcttccttta ttataaatgc aagggctgaa agacgagtaa gtacaacgct ggagcttgta
     6001 gaaattatta aagctgcaat accggcagca gcaaggcgtg aaggcccgca tccggctaaa
     6061 aggactttcc aggcaatcag aattgaagtt aacaatgagc tgggaattct gagagacaca
     6121 attgatgatg cagtttcagt tttaaagacc gggggcagat tatgtataat cagtttccat
     6181 tcccttgagg acaggatagt gaagacagag ttccagagac ttgtaaatcc ttgcagttgc
     6241 cctgtagact ttcccatatg tgtatgtggt aaaaggccgg ttgctatact tactgtgaag
     6301 aaacccattt taccttcaga gagggaaata tgcagcaatc ctagagcaag aagtgcaaaa
     6361 ctaagagtaa tagagaaatt atagaaacta aagacaaaaa taagtatgaa atacaaaaga
     6421 taaaacgttc gtaaagaatg caaaagaaaa aatacaagta aagattacaa aagaaaaaac
     6481 ataagcaaag aatgttaata caaaaaataa gcaaatatac agaagataaa ataccagtaa
     6541 ggaatacgaa agataagact gtagaaaaga tatgactata gaaaagatac taaagataag
     6601 gagttatgct aaattacacg aggccggatt tcgaatgaga tccggctata tcttcctgga
     6661 ataagttaat aataagcaaa taatgaatgt aataaattgc cgtagaggtg aatacattgg
     6721 tacaaaagaa taataaatat gtgtatggta cagcagctga aaaaattcaa tatgatgtat
     6781 atgagaaaaa taaggtcctt aaagaaaaga agcaatacaa agcaaataat gctataaaac
     6841 tgaaaatggt agcatctatt ttattagctt tccttattgg atttgctgtt atgtacaggt
     6901 atgctctgat agctgacatg aattacaaaa tcagcaatat ggaaagacaa tatgaaaagc
     6961 tgaagaatga aaattccagg cttaaagttg caatagaaaa tgaaaccaac ctttcaagga
     7021 tttctgaaat tgcacagaat gagcttggaa tgcaaaaacc tgataagtat cagatagtac
     7081 atataagagt tccaaaaacc agttatacag taatttctga tgcatataaa aatactacag
     7141 caaaagaaaa ccttttcgtt gaattagttc gaaagggagt agcaattaaa aagctattta
     7201 actaaaataa aagtctgcta ttttatacaa ttatatgaaa ctcattataa tttcaggctg
     7261 tgatatgttt tcactgcctt taattttaag caggtttggg gggacattct gtggcaggac
     7321 caggcttaca ggcaaaaaaa aggttattaa ttgttttgat aggattcttt ttgatattcg
     7381 ttattctatt gggacatgta ggatatatcc agatagtaca gggtcagata ttaaaaagta
     7441 tggcctatga acaacaaaca agaggaagag tcataactcc caaaagaggg acgatatatg
     7501 acagaaatgg taagcaactt gcagtgagtg catcagttga tactattgca gtaaatccaa
     7561 aggatgtagc gaaatcaggt atttcaaagg aaataatagc aaagaagctc agtgaaatac
     7621 ttgaaattga tgaggagaaa ataatagcca agcttgaaaa aaatacatgg tatgaagaga
     7681 taaaggaaaa agttgacaag aatattggtg atgaagtaag aaaatggata aaggatgaaa
     7741 aaattgaagg tgtacgtgtt gatgaagatt caaaaaggta ctatccacat aggaatctgg
     7801 catctcacat tattggattt attggtaagg atcaggggct ggatggcatt gaagctataa
     7861 tggaaaagta tctgaaagga gttcccggta aaatattaag tgagatggat gcaggggggc
     7921 gaacaatgcc ttatactccg gagagaaggg ttgatactca ggatggtctt gatgttgtac
     7981 tgaccataga tgaaaccata caatatcttg cagaaaaagc aatagagaaa gcaattgatg
     8041 acaataaagt gctaaatggt gccgtagtaa tagttatggc ccctagaacc ggtgatattc
     8101 tggcattggt atccaaaccg gattatgacc ttaataatcc ctttgcagca ccccctggag
     8161 ttgaccctga aaaatggaag ggttatacta atgaagatgt tacaaaactc caggagacag
     8221 tatggcggaa caaggctgta tcagatactt atgagccggg ttcaactttt aaagcgataa
     8281 ctgctgcagc agctctggaa gagggagtaa taaaacccga ttctcctgta aatgatttcc
     8341 cggtaaaggt gggaggctgg aacatcggat gctggagggc agctcctcat gggcaggaaa
     8401 ccttcaggga aggcgtctat aattcatgca accctgtgtt tgtaagagtt gctcaggatt
     8461 taggaatcaa aactttttat gattatgtca gggggtttgg tttttttgaa ccaacgggtg
     8521 tagaattacc aggggaatct aattctatat ttcacaaacc tcctgtagag atagatatgg
     8581 cagttgcatc atttggccag agatttcaaa ttacaccaat acagcttata actgcttatt
     8641 gcgcaatagc aaatggcggt taccttatga aaccgagact gataaaagaa ctaagagatt
     8701 cccagggaaa tattgtaaaa aaatttgaac cagaagtagt aagacaggtt atttccagtg
     8761 aaacatcaga tacattaagg gatattttgg agggtgttgt ttctgatggt accggaagaa
     8821 atgcctatat aaaaggttat agagttgcag gtaagactgg tacttcagaa actacagaaa
     8881 ctaaatcaga gggaagatat attgcatctt tctcatcctt cgctcctgca gataatcctg
     8941 tagtaaatgt cctcatagtg cttgatcatc ctgcagggga ttcctatatg ggtggagtta
     9001 ttgctgcacc ggtagcacaa aaactacttg atgatgttct gaattatctg ggtgtagaaa
     9061 aaagatatac tgaaaaagat aaagaaacta taagattacc tgtttatgtg cctgaagtaa
     9121 gaaatatatc acttaatgat gcaaaatcta cattagcgaa atatggtttg gaatatagag
     9181 tggaaggtga tggcaataat aaagatgcga ttattttgga gcagatgccg aaacctggcg
     9241 caagtatacc tgagaaatca gttgtgatat tatatacata taaacctgat actgaagtta
     9301 tggctaaagt tccgaatctt ttaaacaaaa ccgtatatga agcaacccag gcattaaaga
     9361 atgcaggctt aaatattaaa gtgaatggcg atggaactgc tgtgaagcag gaaattgtcc
     9421 ctgggtctga agtgcctgtt gggaaggtaa tagaagttga ttttaggcat ctggacaatg
     9481 tagagtagag atgtgtataa gatacccttg ataacgcagc atactataat taattttaaa
     9541 attggaggta gcctatgaaa ctgcatgaat taattgaagg actaaaccct aatgtaatct
     9601 caggagattg tgatttggat ataagagata tagtatatga ttccagaaaa gcaggaaaaa
     9661 attctttatt tgtatgtata gacggctctg tcgttgatgg gcatgcattt atcccggaag
     9721 caattgaaaa tggagcagga gcactgctgg tagaaaaaga ggtaaatgtt ccccccaata
     9781 ttaccgtaat aaaagtaaaa gacacaagat atggacttgc ttttgtttca gataaaattt
     9841 atgaccatcc ctcaagaaaa ctaaatttaa ttggtattac aggtacaaag gggaagacta
     9901 caactacata catgattaag catattcttg aagacgcggg tctaaaaaca ggacttatag
     9961 gtacaataca gaatatgata ggcgatgagg tgctgtacgc agaaagaaca acaccggaat
    10021 cttacgactt acaggctctg ttttctgaaa tgtgtgaaaa agacgtaaaa agtgttgtta
    10081 tggaagtgtc ttctcaagga cttcaactca acagagtaag ttgcagccat tttgaaatag
    10141 gagtatttac caatctttat aaagaccata tctcacctac agagcataaa gatatggagg
    10201 agtatcttaa cgcaaaaata aagctgttta aaatgtgtga aaaagggctg gttaatatag
    10261 atagtccata tgcagcaaaa gttttagctg aggctgaatg tcccatatat acttttggta
    10321 tagaaaatga ttgtgatata agagcttatg atattataaa gcatccacac agtgtggaat
    10381 tccaagtttg ttcaccatgg gtgaatggtc acattcttgt taatatacct ggtaaatttt
    10441 ctgtatataa tgcgttagca gcaataggag tttgctgttt atccggagta tccttcgata
    10501 atattaaaaa tggtcttaaa agtattagtg tgcctggaag agctgaaaca attcatacgg
    10561 ggaggaactt tactgttatt gtagattatg ctcatacggc ggatagcctt gaaaacattc
    10621 tgagtactgt taaggatttc tcaaagggta gagttgtcag tatatttgga tgtggaggga
    10681 acaaagataa gggaagaaga ttcggcatgg gagaagtatc aggcaaaata gcagatttta
    10741 ctgtaattac ttcagataat ccaagatctg aagaccctgc agctataatc aatgatatag
    10801 aaacaggaat aaaaaagaca gaaggtaaat atataaaaat agtggatcgc cgagaagcaa
    10861 taaggtatgc aattgaaagt gccaaacccg aggacataat agtaatagca ggtaagggtc
    10921 atgaaacata tcagatattc aaagacaaaa caatacattt tgatgacagg gaagtagtaa
    10981 gggaaatatt gagtgaaacc gcttcagaaa aaagtgaaga aatagtttta cagaaagagt
    11041 gaagaaacta tttctgagaa gtattcaggg aatagcttcg atacgatagc ggaggtatat
    11101 tatgaaaata ttaagatgcg gggaaattgt agaagctaca gaaggtattt tattatatgg
    11161 cagcagtgag caggaaataa tgagaatttc taccgactcc aggtcggtac agccaggaag
    11221 cctttttgtc ccaataaaag gagaaaggtt tgatggtcac aatttcatta aagacgcttt
    11281 agcagctggt gctgcaggta ctttaaccca atacgattta caggaattaa agtgctgtcc
    11341 tgagcatgag ggctcttttg atggaaaact aattatcaaa gttaatgaca ctttaaaagc
    11401 actaagagat attgctgcat attacagaca aatgttccgt attcccttca caggtattac
    11461 cggaagtgtc ggaaagacaa gtaccaagga tatgatagct tgtgtccttg agcaggaata
    11521 taatgtactt aagactatgg gaaattttaa taatgagatt ggtgttccgt taactatatt
    11581 caacttgaat gatagttatg aagcagcagt tgttgaaatg ggaatgagcg gttttggaga
    11641 aataagcagg cttacttcca ttgtcaaacc caaaatagcg gtaataacaa atattggcat
    11701 gtcccatatg gaaaaactag gatcaaggca gaatattcta aaagcaaaaa tggagatatt
    11761 tgaaggctta ccccgagacg gactggttgt aatgaatgga gatgatactt ttttatacgg
    11821 cttgaagggt tttttaaact ttcgcacatt atattatggc atggatgagg ggttggatta
    11881 tcaggcttat aatgtccaga gtgcagggga aacaggcatt tattttgata ttaatgtaaa
    11941 aaatgtggaa tacaaggttc atgttcctgt tccaggtata cataacgtat ataatgcact
    12001 ggcagcaata gctgtaggag ttgaatccgg catttcaatg aaaaatataa tacaaggaat
    12061 ccgtaatttc atacctggca aaatgaggtt aaacattgtt aaaaacaatg gaatcaaggt
    12121 tattaacgat gcatacaatg caagtcccca atcaatagat gcagctttaa aagtgttaat
    12181 tgatcttgaa gatgtaaaca ggcgtattgc tgttttaggt gatgtcctgg aactaggtga
    12241 atgggctcgg caggctcatc tagatatagg gaaacttgca tttaatatgg gaatagatta
    12301 cattataact gtaggggaaa atggaagata tatcgcagaa ggtgccctgg agtcaggtgc
    12361 ttctgttaaa aatgtaaatc atttttccga taacaaagaa gtaacaagat ttttggaaga
    12421 ctttattact gatggtgatg ccatacttgt aaaaggatcc aggggaatga aaatggaaga
    12481 aatagttgaa caactgataa gaggtgaaaa tgcttgaatg aatactttaa agtatctata
    12541 caggttgttg catttatatt aacttttata ttatctttga ttgcgggacc aataattata
    12601 cctatgctaa gaaggcttaa atttggtcag actgtaagag atgatggacc atctacgcat
    12661 attaagaaaa cgggtacacc aacaataggc ggggttatat ttattatacc aataactctt
    12721 gtatctgttt atttttctgc aaaatatcct gatattatac cattaataat agtaactctg
    12781 gggtttggag cagttggttt tattgatgac ttccttaaag ttgtaaaaaa aagcaaggat
    12841 ggattatttt ggaaacagaa aatgtttgct ttgcttgtta ttgcagtagc atttactatg
    12901 tatggtatta acaaaggaat attaaatact gaaatgatag tacctttcat gggcatggat
    12961 tatacagtag cgttgccaat gtggtttttc ataccattta cgatttttgt cctactcgcc
    13021 attaccaacg ctgtcaatct gactgatgga gttgatggtt tggcaacagg tgtaacatta
    13081 atagttgtgg tattattcac cattattgcc atgacccgca gtgactggga ttacttgaaa
    13141 atattttctt caactgttgc cggaggatgc cttggatttt tagcatttaa tgcctatcct
    13201 gccagagtct ttatgggcga tacgggatcc cttgctcttg gaggtgcggt tggagctata
    13261 tcgattttaa tgaaaatgcc atggattata cttgttgcag gttttattta tgtaatagaa
    13321 gcattatctg ttatcataca ggtttcatat tttaaactta aaggcaaaag agtatttaga
    13381 atggcaccta tacatcatca ttttgagctt ttaggatgga aagaaacaaa ggtggtatca
    13441 gtattttggg tgattacttt tgtttcctgt ataataggat tattgacctt aaggctaaag
    13501 ttcttttaaa aaaaactatt gggggtagca tatatgaaag gaagaaaacc ttttgatttt
    13561 tggatcttta tgacagtgct tatattgcta tctattggca taataacagt tttcagcgca
    13621 agtgcaccgt tttcctacag caactttcgc gatatatatt atatacttaa aaaacagctg
    13681 ttatatgggg cgatagggct tgttgcaatg tttgttgcca tgaatataga ttacagaata
    13741 ttagcgaaat attcatcctt acttcttatt ataagtatag ttatgttaat agctgtactt
    13801 attccaggca tagggaaaga agaaaatgga tcaacaagat ggattgagtt tggttctttg
    13861 cgttttcagc cttcagaaat cgcaaagata gcaataatac tatatttttc ctctagttta
    13921 tctaaaagaa aatccccttt aaattcattt ttcaaggatc ttttacctta tcttatacta
    13981 ataggtgtta tttccggact cttacttctt gaaccacatt tgagctgcac tataattata
    14041 ttaatagtgg ctatgatcat tctcttttca gcaggtgcaa aaataaagca tttcatgata
    14101 ttatttgttc cagctgcttc tgcttttgca gctctagtag cttttgtacc atatatgaat
    14161 agaagagttc tttcttttct agatccatgg aaagatatgc agggagatgg ttatcatgca
    14221 gttcaatctc tatatgccat aggttcaggc gggctgtttg gaagaggttt aggaaagagt
    14281 atgcagaaat ttctttatct gccatatccc cataatgatt ttattttttc agtgttggca
    14341 gaagagttgg gctttttagg agtgctaacc gttatggttt tgtttttaat gtttacatgg
    14401 agaggaataa aagtagccat gagtgcaccg gatctatttg gaagctatac tgctgtagga
    14461 ataacatcac taataagtgt gcagacagta cttaatattg ctgttgtatc ttcagcaata
    14521 cctcctacag gtgtatctct tcctctgttt agctatggtg gaacatcact tgttttgata
    14581 atgggggcta taggtatact cttaagtata tcacggtata cgagttatgg gaggatataa
    14641 gactagaagg ggtgcatatt tgcttatcct acgctcagat taaaaggctc tacagcagct
    14701 tcacttatgc tccttttgat actttgttaa atctgctcaa tttcccacct cccacttctc
    14761 acctctaaac tgagtaacac cttttaaaca cagcgcataa tatggtaatg tatgcgaggt
    14821 gtttaaaatg agcaagatca taataaatgg tggtagaaaa ttaaagggcg aaatatctgt
    14881 agaaggctca aaaaatgcga ttttaccaat attggctgca acacttttaa atagcggaag
    14941 aagtgtgata aggaattgtc cgaaactgaa ggatgttgaa gtaactctta ggatactaat
    15001 gaaattgggt tgcaaagttg aagttgataa agatactgtt atggtagatt cctccaatat
    15061 taccagcaca gaaatacccg tagatctggc tgcggaaatg agatcatcca taatttttat
    15121 gggacctatg cttgcaaggt gcgggagggt tgtaataagc tttccgggag gatgcgagat
    15181 tggcccgagg cccattgatt tgcatcttaa aggattaaga agtatggggg ccaatatact
    15241 tgatgttcaa gggggattta tttattgcaa tgctgaccat cttaaaggta ctgaaatcca
    15301 tttaagctat ccaagtgttg gtgctacaga aaatataatg ttagcctcag tatttgcaga
    15361 aggagaaaca tacatacata atgcggccaa agaaccggaa ataacagatc ttcagaattt
    15421 cctgagagct atgggagctg atgtttcagg agctggttca ggaataatct gcgtaaaggg
    15481 tacggcacaa aagcttaagg atgtggaata cactataatg ccggatagaa ttgtagcagg
    15541 aacatatatg gttgctgctg cgataacggg aggagaagta actttaaata atgtcatgcc
    15601 tgagcatctt agtgcggtaa tttccgcttt aagcgaagcg ggatgtacta tcaaaaatga
    15661 tgaaaaccat atttatatat gcgggccgga tagaaatgaa gcagttgata ttataagaac
    15721 actcccctac ccgggttttc ctactgatat gcaggctccc tttgtttcgc tgttaactgt
    15781 ttgcagaggt acaagtatag ttgttgagac agttttcgaa aacagataca agcatgttga
    15841 tgaattaatg agaatgggtg caaatataac attggaaggc cggattgcag ttatcaaggg
    15901 agttgataaa ttaaatggag cggttgtata tgcaagagat ttgcgtggag ctgcagcatt
    15961 ggcaattgca gggctagctg cgaaaggaga aactgttgtg ttagatatta agcatcttga
    16021 tagaggctat gaaaatatgg aagttaaact agcacaatta ggagcattaa ttaggagaca
    16081 agattaagag attaggaagt catattatgc atcaggataa aaagtataaa ataaatacga
    16141 atacgggaaa tctaaaatat aaagataaaa gcatcaggca cataagaaag aaattggagt
    16201 atattacaat tctttttcta attgttgcag gtctcatagt gcttataatg tcgccaatat
    16261 ttaatatttc taatgtatat atatatggta atagtcatta tagtacagag gaaatatcg
//
LOCUS       DAOSDD010000090        16251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1449_length_16251_cov_4.989318, whole genome shotgun sequence.
ACCESSION   DAOSDD010000090 DAOSDD010000000
VERSION     DAOSDD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16251
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_16251_cov_4.989318"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PK733_09470"
     CDS             <1..825
                     /locus_tag="PK733_09470"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOJ10808.1"
                     /translation="TPHLQGKSIVTLFYENSTRTRLSFELASKYMGASSANISASSSS
                     VAKGETLIDTGRTIERMGTDVIIIRHPMSGAPALLAKNVKSSVINAGDGMNEHPTQAL
                     LDMFTIKEARGNLNGLKVAIIGDIYHSRVARSNIWGMTKLGAEVCVAGPATLLPPCLE
                     KTGIRVYSTVQEALIDADVVMGLRIQLERQKKGLFPTIREYARFFGLDEKRLKLAKED
                     ALILHPGPVNRGVELTTSVIDSDSSLINEQVTNGVAIRMALLYLLTRRSGMVEAVN"
     gene            809..2095
                     /locus_tag="PK733_09475"
     CDS             809..2095
                     /locus_tag="PK733_09475"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOJ10809.1"
                     /translation="MKLLIKNGHVVDARTERNGIFDILIDEGKIQEIGNGLDDASCDF
                     IDASGKYILPGFVDAHCHLRDPGYEYKEDIESGSASAAKGGFTSIACMPNTNPPIDNQ
                     AVIKYILNKAKQEAYVNIYPIGTITKGQKGEELSEIGELKFAGAVAISDDGKPVMNPS
                     LMKKALQYASMFDITVISHCEDLDLADEGVMNEGYQSTIMGLKGIPSAAESVMVAREL
                     LLAEYTNTSIHIAHVSTEASVDLIRNAKRRGVKVTAETCPHYFSLTEDACEGFNTIAK
                     VNPPLRTQKDVKAVIEGLQDGTIDIIATDHAPHHIDEKNVEFNLASNGIVGFETALPL
                     VITYLVRPGILTMDQIVKKMCYNPSKILGLNKGVLEEGRTADITIIDINEEQTINTNK
                     FKSKSKNSPYNGFKLNGVVYHTIVNGRVVVREKVLL"
     gene            2113..3054
                     /gene="pyrF"
                     /locus_tag="PK733_09480"
     CDS             2113..3054
                     /gene="pyrF"
                     /locus_tag="PK733_09480"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOJ10810.1"
                     /translation="MFIDNLIKQIKEKNNPTVVGLDPRIDYVPSFIKEKAFNTYGKNL
                     KGCSEAIYEFNRKIIDAIADIVPALKLQMAYYELYGFYGIEVLNKTIQYGRSKGLIII
                     CDGKRNDIGSTAEAYSSAYLGKVNIDDGIEEAAFDADALTVNPFLGYDGIKPFNDDCR
                     KYGKGIFVLVKTSNKSSGQLQDLLTHQGKSVYEIIAEYVNEWGRNLLGEYGYSSVGAV
                     VGATYPNQAKILRKIMKNAYILVPGYGAQGGTARDVIHSFNCDGLGAIINASRSIMCA
                     HNSDIWKDQYDESRFDEAARAEVIRMRDDINNELNAR"
     gene            3140..3925
                     /locus_tag="PK733_09485"
     CDS             3140..3925
                     /locus_tag="PK733_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOJ10811.1"
                     /translation="MSCILMEKIISNREVCPDIFEMTIRSDYIAQRAKPGQFINIKCC
                     EGLNTILRRPISICSVNRNGSTITILFQKKGTGTEYLSKKNPGDFLDLIGPLGGSFDL
                     SDQYEKIAVIGGGIGIFPLLFLLKESKSIHKTAFIGFRNKDNSVLMDDFKVNTHELLV
                     STDDGSMGYKGFITDLLEERIMTEKYDIIYTCGPELMMKKAVDTAIKYGIKCQASLEQ
                     RMGCGIGACLACACKIKTGDGWQYKRVCKDGPVFWGEEVEFRG"
     gene            3933..4838
                     /locus_tag="PK733_09490"
     CDS             3933..4838
                     /locus_tag="PK733_09490"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOJ10812.1"
                     /translation="MEVEIAGIRLKNPVIAASGTFGFGREYSNYINLNKLGGISVKGL
                     TLKPRKGNEPPRIAETPAGILNSIGLQNPGVKGFIEEELPFLKQFNTAIIANIAGNTI
                     EEYCEIAEILSDTDIDAFELNVSCPNVKMGCVAFGNTTGGIAEITRQVRKYCKKPLIV
                     KLTPSVLDIKEIALAAEINGADAVSLINTLLGMVIDVHKKRPVLANNFGGLSGPAVKP
                     VAVRMTYEVSQTVKIPVIGMGGISCGEDAVEFMLAGASAVMVGTWNFVNPNTCIDVVN
                     GIEKYLDEYNHGSIYDIIGKIQMNG"
     gene            4843..5568
                     /locus_tag="PK733_09495"
     CDS             4843..5568
                     /locus_tag="PK733_09495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ10813.1"
                     /translation="MVTRLILVRHAEAEGNINRVFHGWTDSEITEKGHAQAKLIAQYL
                     RDTDIDVLYSSSLKRTLQTAEYIAKAKGLPIIRTDKLKEINGGNWEDVAWDILPKKWP
                     QEYYSWENEPHAHKMPGGESMKEFQRRLIEELKYIISSNKGNNICIVTHGTAIRSLMC
                     YFHHCDLEEMVGFPWVDNTSFTIVDYEDDKDDKDNKDCEGGQFTVITEGNTSHLSQEM
                     KTLGNQEWWQKNIKDKKGVSAYE"
     gene            5561..6424
                     /locus_tag="PK733_09500"
     CDS             5561..6424
                     /locus_tag="PK733_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOJ10814.1"
                     /translation="MSNKLVQWLFKTDALRVSSADKPFWYTSGTIGPYYINTHFLYGS
                     EKKATELLEFINEAKEDICSFPLKLTELLWENYNKDAIFKGLIDHMCIFIKNNININE
                     VDYISGGERRDWFFSFLVAQLLEKPHLTIYKDLSINEFYKNKVNTITDLQNKNVLHIA
                     DLVTEASSYERAWIPAINRINGIIKWSVVVVDRKQGGKELLARMGVNLYPMVCIDRGT
                     FDNALNMGLIDEKQYNMIIDFINDPKGSMKRFLIGNPDFLENALTSGGKDAERAKLCI
                     EKNIYGLEKGA"
     gene            6433..7851
                     /gene="miaB"
                     /locus_tag="PK733_09505"
     CDS             6433..7851
                     /gene="miaB"
                     /locus_tag="PK733_09505"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ10815.1"
                     /translation="MEKRENITVSLEEIKAQKKYTSLVKQINQGSVKKYHITTFGCQM
                     NENDSEKLAGMLSLMGYEETDDINESDIILYNTCCVRENAEMKVFGHLGALKKVKREN
                     PDTVIAVCGCMMQQEEVVEKIKKKYRNVDLIFGTHNLYKFPELLYSVLKDKHRLYDIW
                     WDTEGQIAEDLPVLRKDGIKAWVSIMYGCNNFCSYCIVPYVRGRERSRRPEDIINEVR
                     MLGQQGYKEITLLGQNVNSYGKDIEGYPDFSKLLYEVDAIETIERIRFMTSHPKDLSD
                     DLIKAMRECKKVCEHLHLPVQSGSSRILKEMNRRYTKEKYLELIDKVKVNIPGISITT
                     DIIVGFPGENDEDFQDTLDVIKRVQYDTAYTFIYSIRKGTPAADRKDQVPEEVKKARF
                     EELLEVQNAISKKINDKLLGKDLEVLVEGPSKTNPGIYTGRTCTNKIVNFQGNEDLII
                     GSLRTVKIEKVQTWSLEGTLLK"
     gene            7861..10482
                     /gene="mutS"
                     /locus_tag="PK733_09510"
     CDS             7861..10482
                     /gene="mutS"
                     /locus_tag="PK733_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ10816.1"
                     /translation="MTNLTPMMQQYLDIKEQYNDCILFFRLGDFYEMFFQDAEIASKE
                     LEITLTGRDCGLDERAPMCGVPFHSVDPYISKLINKGYKVAICEQTEDPALAKGLVKR
                     EVIRVVTPGTVIESSMLDERKNNFLLSIFKNKVFFGIAAVDITTGEFLTTQIIWGNTV
                     VKLLDEVAKYLPSEIIVNTELYNDKSIINQITARFNTYITPFADEYFEVSSAIERIKS
                     GFEESDILKNEGELSINASGALLAYLEQTQKVSLTHIRKIESYHIEEYMAIDVSTRRH
                     LELSETMRDKTRKGSLLWVLDRTVTSMGGRTLRKWIEQPLIKIPDIQERLDAVAEMKD
                     KYMVRMEIRELLKKVYDIERLMSKVIIGNVNCRDLVALKESIGQVPYIKNILENCSAD
                     FNIKNYEKMDNLEDVYELIEKSIVDDPPILVKEGGIIKDGYNEEVDKLRQASTNGKRW
                     IADLEASERAKTGIKNLKVGFNKVFGYYIEVTKSYYSLVPDYYVRKQTLANCERYIIQ
                     ELKEIEDTILGAEEKVVELEYRIFNEVKEEIASQVGRIKSTASSLAETDVICSLAEVA
                     DRESYCMPEVNEGGELVIVDGRHPVVEKMINEGTFVPNDTFLDMEKNRVSIITGPNMA
                     GKSTYMRQVALIVLMAQIGSFVPADSAKIGVVDKIFTRVGATDDLAAGQSTFMVEMTE
                     VANILDNATDRSLLILDEVGRGTSTFDGLSIAWAVIEYISDKNKVGARTLFSTHYHEL
                     TDLEDKISGVKNYKVTIEEKGEDIIFLRKIVRGGADESYGIQVARLAGLPQQVIDRAH
                     EILRELEEADISKKEARLRKIRKPLEGQIDIFTYNSFHKKHEELVNEIKKIDFLSLTP
                     IEAMNILYNLQQKVLKA"
     gene            10549..12561
                     /gene="mutL"
                     /locus_tag="PK733_09515"
     CDS             10549..12561
                     /gene="mutL"
                     /locus_tag="PK733_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ10817.1"
                     /translation="MGKIVTLDENTINKIAAGEVVERPASVIKELVENSIDAGSTRIV
                     VEIKKGGIPFIKVIDNGIGIDEDDVEIAFERHSTSKIRHSDDLPSILTLGFRGEALAS
                     IAAVSKVEMITRTQNKHYGIKIIVNGGNVEEIKQTGAPAGTSITIRDLFYNTPARYKF
                     LKRDSTEAGYVSDIVNRIALGHPEISLKFVNNGTALIHTPGNNDLLSTIASIYGTDIT
                     RHLSEINYQDEFIKLIGYAGGSETSRANRNHQSIFINGRYIRSKLITSAIDEAYKTFL
                     MKNKFAFVILKIEINTAAVDVNVHPAKMEVRFSNEQEIFRSIYHAVSNTLLGGAKIPE
                     VKYEKHDINTNIVSEKQQNINFENRNININDKNIISRQNTILNENEYKSEINPRTGTA
                     DTADTANYIHKPALKINKDIYDSNIYTNVNKNTNASTKTNDNADINTNVNYNEISSVN
                     INTTTYHNDNNFNSNTNNNTTSNNKINDIKDARIIGQLFSTYILLQKNDNFFVIDQHA
                     AHERIMFEKMKEKFFNNQQMAQILLVPVVVELTYQELEVLLENKELFTKMGFTFEDFG
                     NNSIILRSIPYKEQGDDGKELFLQLLDYISTYSRSPGIALEEEALYLIACKAAIKANM
                     HLDDLEKFELIKELMMLDNPYTCPHGRPTILKITKDELEKMFKRKI"
     gene            12680..13600
                     /gene="miaA"
                     /locus_tag="PK733_09520"
     CDS             12680..13600
                     /gene="miaA"
                     /locus_tag="PK733_09520"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOJ10818.1"
                     /translation="MNNIIVIAGPTASGKTKVSIELAKAINGELISADSMQVYKYMDI
                     GTAKPTIIERQGIQHYLIDEVYPNEEFSVAKYQSLAINYIKEIFYKGKIPIIAGGTGL
                     YINSLIYNIRFSEIETDWDLRKDLLNIANEKGNEYLHNQLREFDPEAAERIHVNDVKR
                     IIRAIEVFRATNKTMTYHQRISRSEPSPYNYLLFGLTMDRQVLYDKINKRVDLMIEKG
                     LINEVKNLMNMGYDQSHVSMQGLGYKEILWYLKGEASLDEAVYLLKRDTRHYAKRQMT
                     WLRKIKEIVWININEETRDKDIIQHIINFI"
     gene            13756..13998
                     /gene="hfq"
                     /locus_tag="PK733_09525"
     CDS             13756..13998
                     /gene="hfq"
                     /locus_tag="PK733_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOJ10819.1"
                     /translation="MNKSNINLQDVFLNQVRKEHITVVIYLTNGFQLKGIVKGFDNFT
                     VILDSEGKQQLIYKHAISTISPLKVVNLIFNDGVKE"
     gene            complement(14160..14855)
                     /gene="lexA"
                     /locus_tag="PK733_09530"
     CDS             complement(14160..14855)
                     /gene="lexA"
                     /locus_tag="PK733_09530"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOJ10820.1"
                     /translation="MNNNNLNRSDENVKLNDKQKKILNFMIKEVDEKGYPPSVREICK
                     ALDIKSTSTVHRQLELLMKYGFILKNPSKPRAIKIINNAKAESKDMISSISSSMVNCN
                     LFNKEIASVPIIGRVTAGKPILAVENIEDTFPVPVDFTQNATMFMLKVEGESMIEAGI
                     LDGDLVLVKQQHSAENGDIVVALIDDEATIKSFYKEKDYVRLQPQNPYMDPIIVKDNL
                     SVLGKVVGVYRRL"
     gene            15044..15325
                     /locus_tag="PK733_09535"
     CDS             15044..15325
                     /locus_tag="PK733_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ10821.1"
                     /translation="MSRRYILKNKVRFAIFLSTTFLLLSIVFFKTTVDGYKEKTYQEV
                     KIKKGDTLWEIAKANKKGGDIRDFIYNIKEINELSDSIIYPGMVLKIPD"
     gene            15465..>16251
                     /locus_tag="PK733_09540"
     CDS             15465..>16251
                     /locus_tag="PK733_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ10822.1"
                     /translation="MKKSISIWSFPQDMKIVDCIKLAKNAGFEGIELALNETGELGLE
                     STEEEILDYKKAAEEIGIELSSLATGLYWSYSLTSNCTETREKAKNIVRKQLDAAALL
                     GVDTILVVPGAVGVDFVPGSEVIDYDIVYERALEAMKELAPYAESKKVCIGIENVWNK
                     FLLSPLEMRDFIDKIGSGYVGSYFDVGNVIYSGYPEHWIKILGRRIKKVHFKDYRKDV
                     GTLAGFVDLLSGDVNYPAVVDALNKVGYNSFVTAEMIPPYKNYT"
BASE COUNT         5821 a         2293 c         3405 g         4732 t
ORIGIN      
        1 actccgcatc tgcaaggtaa atcaattgtt actttattct atgaaaacag tacaagaaca
       61 aggctttcat tcgagcttgc ttcaaagtac atgggcgcca gttctgctaa tatttctgca
      121 tcttcaagca gtgtagcaaa aggtgaaacc ctcattgata caggtcgcac tatagaaaga
      181 atgggtactg atgtaataat aataaggcac cctatgtcag gagcacctgc attactggcc
      241 aaaaacgtaa aatcctcagt tattaatgcg ggcgatggta tgaatgaaca tccaacgcag
      301 gcacttttgg atatgtttac cataaaagag gccagaggga atttaaatgg ccttaaagta
      361 gccattattg gggatatata tcatagccgt gttgccagaa gcaatatatg gggaatgaca
      421 aagcttgggg cggaagtatg tgtggcagga cctgcaactt tactgcctcc ttgtctggaa
      481 aaaactggga taagagtcta tagtaccgtt caggaagcat taattgatgc tgatgtggtt
      541 atgggattaa gaatacaact tgaacggcag aaaaaagggt tattccccac aataagggaa
      601 tatgccagat tctttggtct tgatgaaaaa cgtcttaaac tggctaaaga agatgccctt
      661 atattacacc cgggaccggt taacaggggg gttgaactta caacatcagt aattgacagt
      721 gatagttctc ttataaacga gcaggtaaca aatggtgtag caataagaat ggcactttta
      781 taccttttaa caagaaggag tggtatggtt gaagctgtta attaaaaatg gacatgtagt
      841 tgatgcaaga actgaaagaa atggaatatt tgatatttta atagatgaag ggaaaattca
      901 ggaaataggt aatggtcttg atgatgcaag ttgtgatttt atagatgctt caggcaagta
      961 tatacttcca ggatttgtgg atgctcactg ccatttaaga gaccctggct atgaatacaa
     1021 ggaagatata gaaagcggtt ctgcaagtgc agcaaaggga ggttttacat ctattgcgtg
     1081 catgcctaat acaaatccgc ctattgataa tcaggcagtc ataaaatata ttttaaataa
     1141 agcaaaacag gaggcatatg ttaatatata tcctataggt acaataacaa agggtcaaaa
     1201 aggtgaagag ctttcggaaa taggtgaatt gaaatttgca ggagctgttg ctatttctga
     1261 cgatggtaaa cctgttatga atccatcatt aatgaaaaaa gccctgcaat atgcatctat
     1321 gtttgatatt actgtcatat cccattgcga agatttggac ctggcagatg aaggtgtaat
     1381 gaatgagggg tatcaatcta caattatggg attaaaagga ataccatctg ctgcagaatc
     1441 tgttatggtt gcaagggagc ttttgctcgc tgaatataca aatacaagca ttcatatagc
     1501 tcatgtaagt acggaagctt cagttgatct tataaggaat gctaagagaa gaggtgtaaa
     1561 agtaactgct gaaacatgcc ctcactactt ctcgttaacc gaagatgcct gtgaaggttt
     1621 taatacaatt gcaaaagtaa acccgcctct tagaacacaa aaagacgtaa aagctgtaat
     1681 agaaggactg caggatggta ctatagatat tatagctact gatcatgcac cgcatcatat
     1741 tgatgaaaaa aatgttgaat ttaatttagc atcaaatggc attgtagggt ttgaaactgc
     1801 cctgcctctt gttattacat acctggtaag gcccggaatt cttacgatgg atcagattgt
     1861 aaaaaaaatg tgttataatc cttctaaaat actcggatta aataaaggtg tacttgagga
     1921 aggacggact gctgatataa ctattataga tattaatgaa gagcaaacca taaatacaaa
     1981 taaattcaaa tcaaaaagca aaaattctcc atataatgga ttcaaattaa atggtgttgt
     2041 ttaccataca atagttaatg gtagagttgt tgttcgagaa aaagtgttgc tttaataaaa
     2101 gggggcagaa acatgtttat agataattta attaaacaaa taaaggaaaa aaacaatcca
     2161 actgttgtag gactagaccc gcggattgac tatgtaccat catttataaa ggaaaaggct
     2221 tttaacacat atggaaagaa tttaaaaggt tgttctgaag ctatatatga atttaacagg
     2281 aaaataattg atgccatagc tgatattgtt cctgctttga agcttcagat ggcatattat
     2341 gagctatatg gattttatgg aattgaagtt ctcaacaaaa ccatacaata tggccgcagt
     2401 aagggactta ttattatttg tgatggcaaa agaaatgata tcggcagtac agcggaagca
     2461 tattcttcag cgtatttggg caaggttaat attgatgatg gtattgaaga agctgctttt
     2521 gatgctgatg cattaacagt taatcctttt ctcggttatg acggtataaa accttttaat
     2581 gatgattgca gaaaatacgg caagggtatt tttgtccttg taaagacctc aaacaaatca
     2641 tcaggacaat tgcaggattt attgacccat cagggcaaaa gtgtttatga aattattgcg
     2701 gagtatgtaa atgagtgggg caggaacctt ttgggggaat acggttatag tagcgtaggt
     2761 gctgtagtag gagctacata cccgaaccag gcaaaaattc tcagaaaaat tatgaaaaat
     2821 gcatatattc ttgtaccagg ctatggagca cagggcggga cagcaagaga tgtaattcat
     2881 tcctttaatt gtgacgggct tggagcaatt attaatgcat cgagaagcat catgtgtgcc
     2941 cacaattctg atatatggaa agaccaatat gatgaaagta ggtttgatga ggcggcaaga
     3001 gcagaagtaa tcagaatgag agatgacata aacaatgagc taaacgcaag gtaaaggtag
     3061 tgggtagtgg atagtgggta gtggatatat aacagataat agagaacaga gaaagaaagc
     3121 agaagaggag aagaagtaca tgtcatgtat attgatggaa aagattatca gtaacagaga
     3181 agtatgtcct gatatttttg aaatgaccat caggtctgat tatattgccc aaagggcaaa
     3241 accgggacag tttataaata ttaagtgctg tgaaggttta aatacgatcc ttagaaggcc
     3301 tataagcata tgttccgtaa atagaaatgg gagcactatc acaatcttat ttcaaaaaaa
     3361 aggaacagga actgaatatc tctcaaaaaa gaaccccggt gattttcttg accttatagg
     3421 tcctcttgga gggtcctttg acctttcgga ccaatatgaa aaaatagctg taataggtgg
     3481 aggtataggc atctttccac tgctttttct gttaaaagaa agtaagagta tacataaaac
     3541 ggctttcatc ggctttagga ataaggacaa ttctgtcttg atggatgact tcaaagtgaa
     3601 tacccatgaa ttactagtgt ctactgatga tggaagcatg ggctacaaag gctttataac
     3661 tgatttgctt gaagaaagaa tcatgactga aaagtatgat ataatatata cctgtgggcc
     3721 tgaacttatg atgaaaaagg ctgtagatac agccatcaag tatggaataa agtgtcaggc
     3781 aagccttgaa cagagaatgg ggtgtggtat aggagcatgt cttgcctgtg cctgcaagat
     3841 aaagacgggt gatggctggc agtataaaag agtctgtaag gatggacctg ttttttgggg
     3901 agaagaggtg gagttccgtg gatgataaaa gaatggaagt agaaatagca ggcataaggc
     3961 ttaaaaatcc agtgattgca gcatccggta cttttggttt tggaagggag tattctaact
     4021 acattaattt aaataaactt ggtgggattt cagtcaaggg tctcactcta aaacccagaa
     4081 aaggaaatga acctccaaga attgctgaaa cacctgcagg aatattgaac agcataggtt
     4141 tgcaaaaccc gggtgttaaa ggatttattg aggaggaact gccatttctc aaacaattta
     4201 atacagctat tattgcaaac attgctggca atactataga agagtattgt gaaattgcag
     4261 aaatactctc tgatacggat attgatgctt ttgaattgaa tgtatcatgt cctaatgtaa
     4321 agatgggctg tgtagctttt gggaacacta cgggcggtat agcagaaata acaaggcagg
     4381 taaggaaata ctgcaaaaag cctcttatag tcaaattgac tcccagtgta ttggatatta
     4441 aggaaatagc ccttgcagca gaaataaatg gagcggatgc agtctctctt attaatactt
     4501 tgttaggaat ggttatagat gtacataaaa aaaggcctgt attggctaat aatttcggcg
     4561 ggctttctgg tcccgcggta aaacctgttg ctgtgcgaat gacatatgaa gtttcacaga
     4621 ctgtgaaaat acctgttata ggtatgggag gtatttcctg tggggaagat gctgttgagt
     4681 ttatgcttgc cggagccagc gctgtaatgg taggtacatg gaactttgtt aaccctaata
     4741 cctgcattga tgttgtgaac ggtatcgaaa aatatttgga tgaatacaat cacggcagca
     4801 tatatgatat aataggtaaa atacaaatga atgggtgaag agatggtaac aagattaatt
     4861 ctagtaaggc atgctgaagc agaaggtaat atcaatagag ttttccatgg atggactgat
     4921 tcagaaataa ctgaaaaggg tcatgctcag gcaaagctta tagcccaata tcttagagat
     4981 actgatattg atgtacttta ttccagcagc ttgaagcgaa cacttcagac agctgaatat
     5041 attgcgaagg caaagggatt accaattatc agaacagaca agctaaagga aataaatgga
     5101 ggaaactggg aggatgttgc ctgggatata ctacctaaaa agtggcctca agagtattat
     5161 tcctgggaaa atgaacctca tgcccacaaa atgcctggcg gtgaatccat gaaggagttt
     5221 cagaggcgtc ttatagagga gcttaagtac atcattagca gtaataaagg taataacatc
     5281 tgtattgtta cacatggtac tgccataaga tctttaatgt gctatttcca tcattgcgat
     5341 ctggaagaaa tggttggttt tccatgggtt gacaacactt cctttacaat agttgattac
     5401 gaagatgata aagatgataa agataataaa gattgtgaag gtgggcagtt tacggttata
     5461 accgaaggta acacttccca ccttagtcag gaaatgaaaa ctcttggcaa ccaggaatgg
     5521 tggcagaaaa atattaaaga taaaaaagga gtgagtgctt atgagtaata aacttgtaca
     5581 gtggcttttt aaaactgatg ctttgagagt aagtagtgca gataaacctt tttggtacac
     5641 atcaggcact ataggccctt attatattaa cactcatttt ctctatggaa gtgaaaaaaa
     5701 ggcaactgaa ttacttgagt ttattaacga ggcaaaggaa gacatatgct cttttccttt
     5761 aaaattaact gaacttttat gggaaaacta caataaagat gctattttta aaggcttaat
     5821 cgatcatatg tgtatattta ttaagaacaa tataaatata aatgaagtcg attatatttc
     5881 aggcggagag agaagagatt ggtttttttc atttttagtt gcccaattac ttgaaaaacc
     5941 tcatttgaca atttataagg acctgagcat taacgaattt tataaaaata aagttaatac
     6001 tattacggac cttcaaaata aaaatgttct tcatatagca gaccttgtaa ctgaagcatc
     6061 aagttatgaa agggcatgga tacctgcaat aaacagaata aatggtataa ttaaatggag
     6121 tgttgttgta gtagatagaa aacagggtgg gaaagaatta cttgctagaa tgggagtgaa
     6181 tttgtacccg atggtatgca ttgatagggg aacctttgat aatgcattaa atatgggttt
     6241 aattgatgag aaacaataca atatgataat tgattttatt aacgatccta agggttctat
     6301 gaaaaggttt ttaattggca accctgattt tcttgaaaat gcattgactt cgggtggaaa
     6361 agatgctgaa agagcaaagc tttgcataga aaaaaacata tatggactag agaaaggcgc
     6421 atgatagagt taatggaaaa aagagaaaat ataactgtta gcctggagga aattaaagca
     6481 caaaaaaaat ataccagcct ggttaaacaa attaaccagg ggtctgttaa aaaatatcat
     6541 attactactt ttggctgtca gatgaacgaa aatgattcgg aaaaacttgc cggcatgctt
     6601 tcattgatgg gatatgaaga aactgatgat attaatgaaa gtgatattat actttataac
     6661 acatgttgcg ttagagaaaa tgccgaaatg aaagtattcg ggcatttggg ggcattgaaa
     6721 aaagttaaga gagaaaaccc cgatacagtt atcgcagttt gcggctgtat gatgcagcag
     6781 gaggaagttg ttgaaaaaat aaagaagaaa taccgtaatg ttgatcttat atttggaacc
     6841 cataaccttt ataagtttcc tgagcttttg tactctgttt taaaggacaa gcacagatta
     6901 tatgatatat ggtgggatac agaaggacaa atcgcagaag acctgcctgt tttacgtaaa
     6961 gatggtatta aagcctgggt aagtattatg tatggatgta ataacttttg ctcatactgt
     7021 attgtacctt atgtaagagg aagagaaaga agcagaagac cggaagacat aattaatgaa
     7081 gtcaggatgt taggacaaca gggctacaag gagattaccc tgctgggtca gaatgtaaat
     7141 tcctatggca aagatattga aggataccct gatttttcaa aattgctata tgaggtagat
     7201 gccatagaaa ctatagaaag aatacgtttt atgacatctc acccgaaaga tttatcagat
     7261 gatttaatta aagctatgag agaatgcaaa aaggtatgcg agcatcttca tcttcctgtt
     7321 caatcaggaa gcagcagaat attaaaggaa atgaatagaa gatatacaaa agaaaaatac
     7381 cttgagttaa tagataaagt aaaagttaat atccccggaa tttcaattac tactgatatt
     7441 attgtaggct ttccggggga gaatgatgaa gattttcagg atacactgga tgtaataaaa
     7501 agggtacaat atgatacggc ttatacattt atttattcca tcagaaaagg tactcccgca
     7561 gcagacagaa aagatcaggt accggaagaa gtgaaaaaag caaggtttga agaacttttg
     7621 gaagttcaga acgcaataag taaaaagatt aatgacaaat tactgggaaa agacctggaa
     7681 gttttagtag aaggaccaag taaaaccaat ccgggaatat acaccggcag aacgtgtact
     7741 aataagattg taaatttcca gggaaatgaa gacctcataa tagggagctt gagaacagta
     7801 aaaattgaga aggtccagac ctggtccctt gagggtacgt tattaaagtg aggtatagga
     7861 atgaccaatt tgacaccaat gatgcagcag tatttagata taaaagagca atataatgac
     7921 tgtattcttt tttttaggtt gggcgatttt tatgaaatgt tttttcagga tgcggaaatt
     7981 gcttcaaaag agttggaaat aactttgaca ggacgggatt gtggtcttga tgaaagggct
     8041 cccatgtgtg gtgtaccttt tcattccgta gacccatata tatccaagct aataaataaa
     8101 ggatataagg tggctatttg tgagcagaca gaagatcctg cacttgctaa aggacttgta
     8161 aaaagagaag ttataagagt tgttacgcct ggtactgtaa ttgaatcatc catgctggat
     8221 gaaagaaaga ataactttct tttatcaata tttaaaaata aggtattctt tggtattgca
     8281 gcagtagata ttacaacagg agaatttttg acaacacaga taatatgggg caacactgtt
     8341 gtaaaacttt tggacgaggt tgcaaaatat ttaccgtcag agatcattgt aaacaccgaa
     8401 ttatataatg ataagagcat tataaaccag ataactgcaa gatttaacac atatattact
     8461 ccttttgcag atgaatattt tgaggtaagc agcgcaatag agcgaataaa atcaggcttt
     8521 gaagaaagtg atattctcaa aaatgaggga gagttatcta ttaatgcctc cggtgcgttg
     8581 ctggcttatc ttgagcaaac acaaaaagta agccttacac acattcggaa aatcgaatca
     8641 tatcatattg aggaatacat ggctatagat gtatctacaa gacgacacct tgaactttcg
     8701 gaaaccatga gggataaaac aagaaaaggt tctctgttat gggtactgga caggaccgtt
     8761 acctcaatgg gagggcgtac tcttagaaaa tggattgagc agcctttaat caaaattccg
     8821 gatattcagg aaagacttga tgcagtggct gaaatgaaag acaaatatat ggttcgcatg
     8881 gaaatcagag agcttttaaa aaaagtttat gatatagaaa gactaatgag caaggtaatt
     8941 atcggtaatg tcaattgcag agacctggtt gcattgaaag aatcaattgg acaggttcct
     9001 tatataaaaa acatattgga gaactgctct gctgatttta atatcaagaa ttatgagaaa
     9061 atggataacc ttgaggatgt ttatgagctt attgaaaaat ccatagtgga tgaccctcct
     9121 attttagtta aagaaggggg cattataaaa gatggatata acgaggaagt tgataagctt
     9181 agacaagctt caacaaatgg caaaaggtgg attgcagacc ttgaagcatc agagagagca
     9241 aaaacaggaa taaaaaactt aaaagtaggt tttaataaag ttttcggtta ttatattgaa
     9301 gtaacaaaat catactattc tcttgtacct gactattatg tacgaaaaca aactcttgca
     9361 aattgtgaaa ggtatataat acaagagtta aaagaaattg aagatacgat tttaggtgca
     9421 gaggaaaagg ttgttgaact ggaatacagg atttttaacg aagttaagga agaaatagct
     9481 tctcaggttg ggagaatcaa aagtacagca tcaagcttag cggaaactga tgttatatgt
     9541 tctcttgctg aagtagcgga cagggaatcc tactgtatgc cggaagtaaa tgaaggcgga
     9601 gagttggtta ttgttgatgg ccgtcaccct gtggtagaaa aaatgataaa tgaggggact
     9661 tttgtcccta atgatacctt ccttgatatg gaaaaaaaca gagtgtctat tattaccggg
     9721 cctaatatgg cgggaaaatc tacttatatg aggcaggtag ccctaatagt tttaatggct
     9781 cagataggca gttttgtacc tgctgattct gccaaaatag gggttgtaga taagatattc
     9841 acaagagtgg gtgctacaga tgatcttgcc gcaggtcaga gcacttttat ggttgaaatg
     9901 acagaggtgg ccaatatact ggataatgcc actgacagga gtcttcttat cttggatgaa
     9961 gtgggcaggg gtacaagcac atttgatgga ttaagtatag catgggcagt tatagaatat
    10021 atatctgata agaataaagt tggggcaaga actctttttt caactcatta tcatgaatta
    10081 acagacctgg aagataaaat atcgggtgta aaaaattata aagtaactat agaagaaaaa
    10141 ggtgaagata ttatattttt aagaaaaata gtccgtggcg gagctgatga aagttatgga
    10201 attcaagtag cacggcttgc agggctgcct cagcaggtaa ttgacagagc tcacgaaata
    10261 ctcagggaac ttgaagaagc tgatataagt aaaaaagaag caaggttaag aaaaatcagg
    10321 aaacctcttg aaggtcagat tgatattttt acatataaca gctttcacaa aaagcatgag
    10381 gaactggtta atgaaattaa gaaaattgat tttttatcac taacacctat tgaggccatg
    10441 aatattctct acaatttgca gcagaaagtg ttaaaagctt aggtgtgtta tatgttaggt
    10501 gtattatatg ttaggtgtgt tatataatga gaaaggtttt ggtttagcgt gggtaaaatt
    10561 gttacgcttg atgaaaatac aataaataaa atagctgcag gcgaagtagt tgaaagaccg
    10621 gcatcggtaa tcaaagagct ggttgagaat tctatagatg caggttctac aagaattgtg
    10681 gtagaaatta aaaaaggagg cattcctttt attaaggtta ttgataatgg catcggtatt
    10741 gatgaagatg atgttgaaat agcttttgaa agacattcta ccagtaagat aaggcattct
    10801 gatgatttac cctccatatt gactcttgga ttccgtggag aagcccttgc aagtattgct
    10861 gccgtttcaa aggttgagat gataacaagg acacagaata aacattatgg cataaaaatc
    10921 atagtgaacg ggggaaatgt tgaagaaatc aagcaaacag gagcacctgc aggtacaagt
    10981 attactatta gagacttgtt ttacaatact ccggcaagat ataagttttt aaaaagggat
    11041 tccacagaag caggatatgt atcagatata gtaaacagga tagccttggg acaccctgag
    11101 atatcattga aatttgtgaa caatggtaca gcattaattc atacacctgg taacaatgat
    11161 ttgttaagca cgattgcaag tatttatggc actgatatta ccaggcatct ttctgaaatc
    11221 aattatcagg atgaatttat aaaactgata ggatatgcag gcggctctga gacttccagg
    11281 gctaatagaa atcatcaatc tattttcatt aatggtagat atataaggag caagttgata
    11341 acatctgcta tagatgaagc ctataagaca tttttaatga aaaataagtt tgcatttgtt
    11401 attttgaaaa tagagataaa tactgctgca gttgatgtaa atgttcatcc cgctaaaatg
    11461 gaggttaggt tttctaatga acaggaaatc ttcaggagta tttaccatgc tgtaagcaac
    11521 actttgctgg gtggtgcaaa aattccggaa gtcaagtatg aaaaacatga tattaataca
    11581 aatatagtta gtgaaaagca gcagaacatt aattttgaaa acagaaatat taatattaat
    11641 gataaaaata taatcagtag acaaaatact attcttaacg aaaatgaata taaatctgaa
    11701 attaaccccc gtacaggtac cgcagatact gcagatactg caaattatat tcataaacca
    11761 gcgttgaaaa tcaataaaga tatatatgat agtaatattt atacaaacgt taataaaaac
    11821 actaatgcaa gcactaaaac aaacgataat gccgatataa atactaatgt aaattataat
    11881 gaaatttcta gcgtaaatat aaatactact acttaccata atgataataa ttttaatagt
    11941 aatactaaca acaatactac tagtaataat aaaataaatg atattaaaga tgcaagaatt
    12001 attggacagt tgttttctac ctatatactt ttgcaaaaaa atgataattt ttttgtaata
    12061 gatcaacacg ctgcacatga aagaattatg ttcgaaaaaa tgaaggaaaa attctttaat
    12121 aatcagcaaa tggcacaaat tctactagtg cctgtagttg ttgaacttac ttatcaagaa
    12181 ttggaagtac tattggaaaa taaagaatta tttacgaaaa tgggttttac ctttgaagat
    12241 tttgggaata attctataat attaaggtct ataccatata aggaacaggg tgatgatgga
    12301 aaagagctat ttcttcagct tcttgattat atttctacat attccagaag tcccggtatt
    12361 gctttggaag aagaagcttt atacctaata gcctgtaaag ctgcaattaa agcaaatatg
    12421 catcttgatg atttggaaaa gtttgagttg ataaaggaac tgatgatgct tgataaccca
    12481 tacacttgcc cccatggcag accaacaata ttaaaaataa ctaaagatga actggagaaa
    12541 atgtttaaga gaaaaatata gtttaagatt agtactataa tgccaatttg aaaagctatg
    12601 tcttctggta taatttttga tagtatcgtg atttcgatgt taaagataaa taaaatacgg
    12661 gtatttatgg aggtttgtgg tgaataatat aattgttata gcaggtccga cggcatctgg
    12721 caaaacaaaa gtatctatag aacttgcaaa ggcaattaat ggtgaactaa tttctgctga
    12781 ttctatgcag gtttataaat atatggatat tggtacagct aagccgacaa taattgagag
    12841 acagggaatt caacattatc ttatagatga ggtttatccg aacgaagagt ttagtgtagc
    12901 aaaataccag tcacttgcga taaactatat taaagaaatc ttttataaag gtaaaattcc
    12961 tattattgcc ggaggtaccg ggctttacat aaattcatta atatataata taagattttc
    13021 tgaaatcgag actgattggg acttgagaaa ggacctactg aatattgcta atgaaaaagg
    13081 gaatgaatat ttgcacaatc agttgagaga atttgatcct gaagctgctg aaagaattca
    13141 tgttaatgat gtaaaaagga ttataagagc tattgaagtc tttagggcta caaataaaac
    13201 catgacttac caccagagaa tatcaagatc tgagccttcg ccatataatt atcttttatt
    13261 tggattaaca atggataggc aagttctcta tgataaaatt aataaaagag ttgaccttat
    13321 gattgaaaaa ggtttgatta acgaggttaa aaacttaatg aacatgggat atgaccaaag
    13381 ccatgtatca atgcaaggtt taggttataa ggaaatatta tggtatctca agggagaagc
    13441 ttctttagat gaagcagttt atcttttgaa aagagataca cgacactatg ccaaacgaca
    13501 aatgacctgg ctcagaaaaa taaaagaaat tgtatggatt aatataaatg aagaaaccag
    13561 ggataaagat attatccaac atattattaa ctttatatga caatgttgta tattatcttg
    13621 aaactattgg tataattcgg tagaatctat ttataactag gcgatagata ttacctatac
    13681 aaaaagattt gtttagtata aagatatatt ttttaactaa actgacatag aaaaagatat
    13741 agaggaggat tttccatgaa caaaagcaac attaatttac aggatgtgtt tttaaatcag
    13801 gtaagaaaag agcatataac agttgtaatt tatcttacca atgggttcca attgaaaggt
    13861 atagtaaagg gattcgacaa ttttacagtt attctggaca gcgagggtaa acagcagctt
    13921 atatataagc atgcaatatc tactataagc ccattaaaag tagtgaacct catatttaac
    13981 gacggtgtaa aagaataaaa atcatcatag tattaagggc gcatttttta attttttaag
    14041 aaatatattc aagaagctat cgttacagtg cttcaccaaa taatattttt aaataattat
    14101 atgcgcccag cataaattta aaataatttt tatgcaatca ggcacttgaa ctgaaatcct
    14161 tataatcttc tatatacacc tacaacctta cccaatacag ataagttgtc ttttacaatg
    14221 attggatcca tataagggtt ctgtggctgt aatcttacat aatctttttc cttgtaaaag
    14281 gattttatcg tagcttcatc atcaattaat gcaactacaa tatcaccatt ttccgctgaa
    14341 tgctgctgct tgaccagaac aaggtcaccg tcaagaattc cagcctcaat catgctttca
    14401 ccctcaactt taagcataaa catcgtggca ttctgagtaa aatctaccgg gacgggaaaa
    14461 gtgtcctcta tattttctac ggcaagtata ggcttacctg ctgttactct gcctataata
    14521 ggaacacttg caatttcttt attaaacaaa ttacagttaa ccatactgga cgaaatgctg
    14581 cttatcatgt ctttactttc agctttagca ttattgatta tttttatagc ccttggtttt
    14641 gagggatttt tgagtatgaa tccatatttc atcaacaact caagttgacg atggaccgtt
    14701 gaagtcgatt ttatgtccag agctttgcaa atttctctaa cagaaggcgg ataacctttc
    14761 tcatctactt ccttaatcat aaaattaagg attttctttt gcttatcatt aagcttcaca
    14821 ttttcatctg acctgtttaa attgttgtta ttcataatta cacctcatat tcatgatatt
    14881 ggagagacct acttatacaa aaataatact ttacatatat tatatcattt cttcagcaat
    14941 tgtcaaacgt gtgttcggaa aatttttaaa aactattgac aacagaacaa gcgtttattt
    15001 ataatagtat tagaacgggc gttccataat aggagggttg tatatgagta gaagatacat
    15061 attaaaaaat aaggtaaggt ttgcaatttt tttatccacg actttcttac ttctatcaat
    15121 tgtttttttt aagacaacag tcgatggtta taaagaaaaa acatatcagg aagtaaagat
    15181 aaaaaaaggt gatacactgt gggaaattgc aaaggcaaac aaaaagggcg gagatattcg
    15241 tgactttatt tataatataa aggaaataaa tgaattatcg gatagtataa tttaccctgg
    15301 aatggtttta aagatacctg attgaagaat gaaaataggt aaacttgctt gcaaaagttt
    15361 taattaattg atattataat gactagtaat cttttaaaat agatatgtct acataaatgc
    15421 tatttttgtt caatatcact actttaatca gggaggtatg taaattgaaa aaaagtatta
    15481 gtatatggtc gtttcctcaa gatatgaaaa tagttgattg tattaaactg gctaaaaatg
    15541 ccgggtttga aggaattgaa ctggcattga acgaaacagg tgaactaggc cttgaaagta
    15601 ctgaggaaga aatattggac tataaaaaag cagcagaaga gattgggatt gaattaagca
    15661 gccttgcaac aggattatat tggtcatatt cacttacaag caactgcacc gaaacaagag
    15721 aaaaggcaaa gaacatagtt agaaagcagc ttgatgcagc tgcattgctt ggagtagata
    15781 caatcctcgt tgtacccgga gcagtaggag ttgatttcgt gcccggcagc gaagttattg
    15841 actatgatat agtttatgaa agggcacttg aagcaatgaa agaacttgct ccttatgctg
    15901 agtcaaagaa agtttgcata ggtatagaaa atgtatggaa taaattcctg ctatctccac
    15961 tggaaatgag ggattttatt gataagattg gttcaggtta tgttggctca tattttgatg
    16021 ttggaaacgt aatatattct ggttacccgg agcattggat taaaattctg ggtagaagaa
    16081 taaagaaagt tcactttaaa gactatagaa aagatgtagg aactttggca ggttttgttg
    16141 atcttctatc tggtgacgta aattatcctg cagtagttga tgccttgaat aaagttggat
    16201 acaatagttt tgttactgcg gaaatgatcc ctccgtataa gaattatact g
//
LOCUS       DAOSDD010000091        16133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1462_length_16133_cov_5.020027, whole genome shotgun sequence.
ACCESSION   DAOSDD010000091 DAOSDD010000000
VERSION     DAOSDD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1462_length_16133_cov_5.020027"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PK733_09545"
     CDS             complement(<1..1305)
                     /locus_tag="PK733_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10823.1"
                     /translation="MKNVVERNYEFRKRLLEVHKQDRRDYNIVPQQDEIVVDDTWDIA
                     IPDNADRVILNAAKDLQDYFFTSMGVSLKLIRTNNIEKEAESGLKKIIFITKEIAKKL
                     GKCLDTPRSYHFSCSDDRIILCGYDERGAAQASYYLEDLMNFKCAPIVKKGEECRKPL
                     FSPRMVHSGYGLDMFPDAHLNAIAHAGMDAILVFTKDADTTPHGYLDFNELIYRAAGY
                     GIDVYAYSYLKSLKHPDDADAESYYENTYGKVFDKCPGLKGIVMVGESVEFPSKDSHT
                     SGKFHRENTEDGLPTGKPSPGWWPCQDYPQWLNLVKKVIRQHKPEADIVFWTYNWGYV
                     SKEYRIELLKNIPTDISLLVTFEMFEQVKTGNITSTCVDYTLFFEGPGKYFISEAEIA
                     HERGIKLYTMCNTGGLTWDIGVIPYEPAPYQWMRRYKGLHDAR"
     gene            complement(1472..2206)
                     /locus_tag="PK733_09550"
     CDS             complement(1472..2206)
                     /locus_tag="PK733_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ10824.1"
                     /translation="MKLEYIFPKDVDKAKAEKWPLVIPVGTIEYHGPHCSFGCDTLIA
                     YGLLERLEKEKDIIIAPPVWYSPASYAVAGPEKGTVHVGADIFEQNIYCILKSLLYGG
                     WRNIYLLIHHQYEQENLLPMTLSCMKAAKKLTFEFLEDNRGIGWWGSNSNKEFYDQLD
                     GQDNPWNWITVLPCMSREVQSATGYDHAGKWECSILSALYPETVVKEYIKDSDEWFIQ
                     DAVESSPKIGEKMVSLCLDDLRKKIK"
     gene            complement(2459..3628)
                     /locus_tag="PK733_09555"
     CDS             complement(2459..3628)
                     /locus_tag="PK733_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOJ10825.1"
                     /translation="MSRIMIPDWEYKERVKKASKLAGEKGLDIFLVNSNEADYANARY
                     FSGFWPLFERAGVAITPEGNAALIVGPESVIFASDVSRIEKIFTSLDYRESADPSYPE
                     TKTSTYKDIFNALGVKGEKIKIGIGSFLDTNAVMLESLRANYPEAEIVKADDIMLNLR
                     GIKSENELACMREGFRITELAIQEVIKNLKPGVTELQMVGIAQRVIYENGAEYEGLPM
                     YVFSEKSTRHAISRSSHRIIEKGDLVQLNLSAKVDGYSPSVGMPVCVGKLTEEKRTLV
                     EFGLRAHNWTGKQIRAGIPASDIAKGYYDLFVSNGYKDNYVYGPCHGTGMIEVEAPWM
                     ETNSKYLLKPNMTFQIDSFVSGSTFGLRWEKGIVVTEAGCESLCSPIGEIIELDF"
     gene            complement(3814..4194)
                     /locus_tag="PK733_09560"
     CDS             complement(3814..4194)
                     /locus_tag="PK733_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOJ10826.1"
                     /translation="MIKIELNNGKKIWYFPDGELPPPGDEPLKGHESIIILNDNEGTA
                     NVIMTLYFADRDPIEDIKITVTPKRVKCIRMDNPEHLGGVILPRETQYAAKLVSDIPV
                     VIQYGRLDTRQEKMAFYTTMGLSF"
     gene            complement(4424..5410)
                     /locus_tag="PK733_09565"
     CDS             complement(4424..5410)
                     /locus_tag="PK733_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ10827.1"
                     /translation="MNTKLVDIAKHLNISVSTVSRVINGKDRVSDETREKVLNAIKEF
                     NYQPNEIARSLRSRSSMTIGVILPDISNEFFALLIKGAEAVAKNNGYLVILCNSDYDE
                     TMEKEYLNMLAQKQVDGIIVATVCRDGEYFDKILNGSIPAVFVDNLPQVKRNYNFVTI
                     DNEKASYDLTKYLISSGYKDIAIITGKLQETSAIERLDGWKKAMNDSGVKVNNDFIGI
                     GDFKIESGYKTMKKMLELNKKPQALLAANNYIAYGAVKAIREKGLRVPEDVYVVCFDA
                     TDETGLMSIRLPSMIQPAEKIGEIALEIIIKRIDNKEFKIYDNVILEPEFVK"
     gene            complement(5585..7552)
                     /locus_tag="PK733_09570"
     CDS             complement(5585..7552)
                     /locus_tag="PK733_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ10828.1"
                     /translation="MKPMKRAIHLDFHTMPGIYNFNENWDAKVFADTLKEAKVEYINA
                     FAKCNLGFAYYPTKIGIPYPGMKGDMFGDLLRECHKKNIGVTAYFNVGLDHEQARLHR
                     EWCILNKDGQVIYGDRTANFFRNMCYNNKGYRSYIMGMMKEVLDNYDVDGLFLDCMGL
                     WPCYGNECLEDMVNQGLDPLNDAHVREHSEEVLMQFSRDVKKMAGNDKYLYLNGMPYR
                     SVKDLDTHIEIECLPSGWSYDYFGAQAAYARNIQKKVLYMTGRFQRSWGDFGGLKSKA
                     SLEYDMWDAISNGLDVSIGDHMHPAENLEPEVYKVVGEIYSDIEKYEPWTEDASYIAD
                     IGVLTDSSGFLDNTYKGLSRMLGELKYGFDIVNEDMDLTKYQILILPDNMKVTPALKE
                     KLEKHLSDNKGILSTGEGGLNPDKTGFAMTQWKFEYDGLDTSNSSYFRMVKDHDCGIG
                     DMRWAMYSHGILMKAGKEIEILGETRGPAENNETEILAEYIKPYFNRHWDGFHGYYYT
                     PPEKATGHAAVAGSGNIYHICFKVFNAYYESAMLSHKAIVKYCLEKLLPEPSLKCKDI
                     PSTSRVTLTKKEYTVQLHVKATFPEVRGNMDIIEEHQIIPEGAVVSVKGEYKKAYIAP
                     CKTPIEMIQNKNYTDVILPQVKGYIMIVMEK"
     gene            complement(7629..8831)
                     /locus_tag="PK733_09575"
     CDS             complement(7629..8831)
                     /locus_tag="PK733_09575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ10829.1"
                     /translation="MSGISGRNNKLIVGVAGDICMRNLAELMDAQYSKTALSKIQPIL
                     DKADVRLINLENAVIKGGTPIHKSGPPLKANPENLAFLKEGHFDCAILANNHVGDYGE
                     EGVFSTLRSLKKEGIAYVGAGKNLDDSYTPWYYEANNCKLAVIATCENEFGIAEAKKA
                     GAAGFDLYRIIHTIKEAKANADFIMIIMHGGNEYNPIPSPRVVALYRTFVDIGADAVI
                     GMHPHCVQGFETYNNAPIVYSIGNFLFSKFGDEDHKSPWYYGYIPLITFEKGKPAELE
                     INPYRFNQECTLISPFEGEGKSAMLEYLMEISEPLKDKQLLQKFFKGWCIISGIPHAE
                     RVVFKKEFLDETEFPTGHPLLAIRNMYTCEAHNELLTVFLKMLADGKLDIGRSMVDEI
                     RRLQKMPI"
     gene            complement(9337..9684)
                     /locus_tag="PK733_09580"
     CDS             complement(9337..9684)
                     /locus_tag="PK733_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10830.1"
                     /translation="MELKMFIDIHAHAYRKPVPFIVQFCTAEELLKRYDELGIEKGVL
                     LPIVSPEIYLPQTNEDILDMAGKYPDRLIPFCNIDPRALTNSAEAPLDKLLSYYKDKG
                     CKGIGEVMLNVPD"
     gene            complement(9739..10839)
                     /locus_tag="PK733_09585"
     CDS             complement(9739..10839)
                     /locus_tag="PK733_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOJ10831.1"
                     /translation="MHWSTIISLDKSFFSEKEKVLVANQGICASTFLFDSGVHGLRIK
                     NNYGNVVILPYQGQQVWSCNFHGRELAMKSMFSEPVATSEFLKTYGGFLLHCGATAMG
                     VPSEKDKHPQHGELPNAPYQKAYLEAGSDEKGHYISIGGQYHHMVAFNYNYIAEPLIK
                     IYENSSVFNVSMKITNLKKTDMELMYLMHVNFRPIDNGVLIYSADCNPKDVRVHADIP
                     THIKSNPDIIKLVDFLEALKVNPELHHTLTPDLVFDPEILFTINYKADEEGNAHSMLA
                     HPDGYASYISHRPSELEYGLRWISRTKDEDALGIVLPATAEHKGYTAEKEKGNIKIIK
                     GGENVEFHAQAGLLIPYDAERMENKIKNILKK"
     gene            complement(11211..11543)
                     /locus_tag="PK733_09590"
     CDS             complement(11211..11543)
                     /locus_tag="PK733_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOJ10832.1"
                     /translation="MATARINLTVDAAAKEEALKLFDIFGLDASTAVNMFFKSVVRTK
                     SIPFDISIANDKSNIFDMSNNEFMGAVQRAIDTKDDAPERAYTVTMDLNEKKPYKLFN
                     DGRREYIE"
     gene            11922..12389
                     /locus_tag="PK733_09595"
     CDS             11922..12389
                     /locus_tag="PK733_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10833.1"
                     /translation="MSYEKSISEAKQEFTNIVREVGMGYEVIVSNHKSPKRGRVSIIS
                     TDVYEDILKQGFKFNPIIEKDEESKGFTIFLNELLIHGDGPTTYEALFDLASNVMDYA
                     DDYIKRFSFFRQIDNRKSHYPYLRRINKCSDINEVMEVIAECHIDLQQAILRK"
     gene            12350..12595
                     /locus_tag="PK733_09600"
     CDS             12350..12595
                     /locus_tag="PK733_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10834.1"
                     /translation="MPYRFTTGDIKEIAHRLGLHKVNEKKWNGIDINGNFLQTYIHDH
                     GDGVEILTGTAKQHSMQMSFKDLDDMYNFLMNKKRKR"
     assembly_gap    12719..12728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12729..15177)
                     /locus_tag="PK733_09605"
     CDS             complement(<12729..15177)
                     /locus_tag="PK733_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ10835.1"
                     /translation="MKEVYKDFVVVGGGLAGACAAISAARNGLRVALVHERPVLGGNS
                     SSEIRVWSLGAIGGGNHFYEETGIIGEMKLENLYRNPEGNPYLWDMVLLDFVKREKNI
                     ELFLNTSVYKITMNNDKVYEDQADTSQIYEDQLVASQSCANQSNEYRREILSCTGWQL
                     GSELEVTFYSPIFADCTGDGTLGYLAGAEYMMGREGKNEYNENMAPEKADNYTLGSSI
                     FFYTKKTDKKIRYVKPDFAYDWEKIKQILSIGNRIVSVKGSGCDYWWFEYGGIQNTIS
                     DNEAIKDKLQKLVFGIWDYIKNSGEFEADNYTLEWISYIPGKRESRRLLGDYVLNQRD
                     LESQRIFEDAVCYGGWSIDTHPPEGIYSKEPGCKQIKVGAYTIPFRSMYSKNINNLLF
                     AGRNISATHIAFASTRVMNTTAMMGHAIGTAASFCVSNEANSEGNHKINPRGIYENKT
                     MLEAYKNRLLKEDCYIIGMKNSDPLDIAREALVSASSLRVPENTTPANSLIQLTYAKI
                     ANQSISEQATSDRQIDYKYLSPISKKLDLNKGMYITFPAKGRMDAFQILVDVNEDTDL
                     GVILYIPEKPQNFLPEKLIKQQTISINKGTAIWMDIPINLKYTGTMVAKILPNENVKI
                     YESEVSCTGIFCCLHSGMRTTFNPCFKYKINTTVPNTEWSDANLTGPGIGESNSDNIN
                     SIYSPNNIINGYNRPYLLPNLWISDRINADKSQHLDLQFKEEKSINEAILFFNSDLNT
                     ERNNLRPGIWSENDCSMPVELVKEYKIYALNADGQPSRILVHEENNIKRYVKHTLAPG
                     TKAYGIRIEVIKTWGNPY"
     gene            15545..>16133
                     /locus_tag="PK733_09610"
     CDS             15545..>16133
                     /locus_tag="PK733_09610"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOJ10836.1"
                     /translation="MDKLKYAGLLEYLDFISKTYNMEICINDFTGFTMLEPDLMSVLQ
                     PYMIHKNKFCMYLKSNNQLWQKCIKNKKKILDKCNKEKQIFKGICPSGVEEYIVPILY
                     DNMAIGFISVGIYSSNKRISEYLLRKKCLRYNLDFEKALDLYNSEYSSSCHSDQLIRS
                     VFSILVQYFQELYIRHINSITYKHKQQNKQHQLSPQ"
BASE COUNT         4766 a         3288 c         2523 g         5546 t
ORIGIN      
        1 ccgtgcatca tgcaaacctt tatagcgtct catccattga taaggagcag gctcatatgg
       61 tataacccct atatcccagg ttaatcctcc ggtattacac atggtataaa gttttattcc
      121 tctttcatgt gctatttcgg cttcactaat aaagtacttt cccggtccct caaagaatag
      181 agtataatca acacatgtac ttgttatgtt tcctgttttt acctgttcaa acatttcaaa
      241 tgtaacaagc agtgatatat ctgtaggaat attctttaac aattcaattc tgtattcttt
      301 gcttacatac ccccaattgt atgtccagaa tactatatct gcttccggct tatgctgcct
      361 tataaccttt ttcacaagat taagccactg gggataatcc tgacatggcc accagcctgg
      421 acttggcttt ccggttggta aaccatcctc tgtattctct ctatggaact tacctgaagt
      481 atgagaatct ttgctgggaa actcaaccga ctcaccaacc atcacaatac cttttagccc
      541 gggacactta tcgaaaacct ttccatatgt attctcatag tagctttctg catctgcatc
      601 atcaggatgc ttgagactct ttaaatagct ataagcataa acatcaatac cataacctgc
      661 tgctctatat ataagctcat taaaatcgag gtatccgtgt ggagttgtat ctgcatcttt
      721 tgtaaacacc agtatggcat ccatccctgc atgagctatg gcattaagat gggcatccgg
      781 gaacatatcc aaaccatagc cagaatgcac cattctaggt gaaaatagcg gttttctgca
      841 ttcttcacct ttttttacta taggagcaca tttaaaattc atcaaatcct caagataata
      901 agatgcttgt gccgctcctc tttcatcata tccgcataaa attattctgt catctgaaca
      961 tgaaaaatga taactgcgag gtgtatccag acatttgcct agtttttttg ctatttcttt
     1021 cgttatgaat ataatttttt tcaaaccaga ttcagcttct ttttcaatat tattggttct
     1081 gattaactta agcgatactc ccattgaagt gaaaaaataa tcctgcaagt cttttgctgc
     1141 atttaaaata actctatccg cattatcagg tatagctata tcccaagtat catctaccac
     1201 aatttcatcc tgttgtggaa ctatgttgta atctcgtcta tcctgtttat gcacttcaag
     1261 caatcgcttc ctaaattcat aatttctttc taccacattt ttcatattaa tgtaactctc
     1321 ccttccagct acggcaaaac aagttatgga catttacctg taataagaca cgaaaatgtc
     1381 tcatcaaatc tttaaattta atatgataaa cacatatttc aaataaaggt agacacacaa
     1441 aatctatgcc tacctaccct atgctttaag attacttaat cttctttcta agatcatcca
     1501 agcaaagact aaccattttc tctcctattt tcggtgatga ttcaacagcg tcctgaatga
     1561 accactcgtc gctatctttt atatactctt ttacgactgt ctcaggataa agggcactta
     1621 gtattgagca ttcccatttg cctgcatggt catatcctgt tgcggactgt acttccctgc
     1681 tcatgcaagg taatactgta atccagttcc atggattatc ctggccatca agctgatcat
     1741 aaaactcttt gttgctattg ctgccccacc atccaatgcc tctgttatct tcgagaaatt
     1801 caaaggtaag ttttttagct gctttcatac agcttaatgt cattggaaga aggttttcct
     1861 gctcatattg atgatgtata agcaggtata tattacgcca cccgccatag agaagtgatt
     1921 tcaatatgca gtatatgttt tgttcgaata tgtctgcgcc aacatgcact gtaccttttt
     1981 caggcccggc tacagcatag cttgcagggc tataccatac aggaggggca attattatgt
     2041 ctttttcctt ttccagtctt tcaagcagac catatgcaat aagagtatca cagccaaaag
     2101 agcaatgcgg tccatggtac tcaattgttc caacaggaat tacaagcggc catttttcag
     2161 cctttgcttt atcaacgtct tttggaaata tatattccag tttcatattt ttgctccttt
     2221 caaaggcaag tgagaatgac aagccagagc gaaagacaag ggtggacggt tcttgcttgt
     2281 catccagcct gccaccttaa tttatcttga taaccttatc agtattatcc ttataagtat
     2341 tattaaatca ttttggtaaa tgatcatatg gaggagtccc acattgattt agaaccattg
     2401 gcaccgtcct cacatgcgaa ctgcctccac ttgttaccat attgacttac acagcaactt
     2461 aaaaatccaa ttcaataatt tctcctatag gactgcaaag gctttcgcag ccagcttcag
     2521 taacaacaat tcctttttcc catctcagac caaatgtgct tccagataca aaactgtcaa
     2581 tttgaaaagt catattgggt ttaagcagat attttgaatt agtctccatc catggagctt
     2641 ctacttctat cattcctgtt ccatggcaag ggccatatac ataattatcc ttataaccat
     2701 ttgatacaaa taaatcataa taaccttttg caatatcaga tgcgggtatt cctgctctaa
     2761 tttgttttcc cgtccaatta tgtgccctaa gaccgaattc tactaatgtt cttttttcct
     2821 cagtaagttt gccaacacat acaggcattc caacacttgg tgaatagccg tctacttttg
     2881 cggataggtt aagttgtacc aggtctcctt tttctataat tctgtgagat gatctggaaa
     2941 ttgcatgcct ggttgatttt tcactgaaaa catacattgg aagtccttca tactctgcac
     3001 cattttcata tataaccctc tgtgcaatac ctaccatctg caactctgta acaccaggtt
     3061 tcaggttttt gataacttcc tgaattgcca gttcagttat gcggaatcct tctctcatac
     3121 aagccaattc attttctgat tttatacctc gcagattaag cataatatca tcagctttta
     3181 ctatttctgc ttccgggtag ttggctctca ggctctctag cataacagcg ttagtatcaa
     3241 gaaaactgcc tattcctatt tttatttttt ctccttttac accaagagca ttgaatatgt
     3301 ctttatatgt actggtttta gtttctggat aagacgggtc tgctgattca cgatagtcaa
     3361 gtgacgtgaa aattttctcg attctgctta catcagaagc aaatattaca ctttccgggc
     3421 ctacgatcaa agcagcatta ccttcaggtg taatagcaac tccggctctt tcaaataaag
     3481 gccaaaatcc gctaaagtac cttgcatttg cataatctgc ctcattggaa tttacaagga
     3541 atatatcaag tcccttttct cctgcaagct ttgaagcctt tttaaccctt tctttgtatt
     3601 cccaatcagg tatcattatt ctactcatgt gtgaaacctc actttcaatt atataatata
     3661 ttttataaaa taaatactat ctttcctttt cgaggacagg gctatatcca aagcactgaa
     3721 agcatatatc taaaggtata caatcgaacg cataataaat aaaagctggt ctctttacac
     3781 ttgatgcttt gaaaattaaa cttggagcta aagctaaaag cttaatccca tcgttgtgta
     3841 aaacgccatt ttttcctgcc ttgtatcgag cctcccgtat tgtattacta caggtatatc
     3901 gctgacaagt tttgctgcat actgtgtttc acggggtaaa attactcccc caaggtgctc
     3961 aggattgtcc attcgtatgc attttaccct tttaggtgtt acagtaattt taatatcttc
     4021 aattgggtcc ctgtccgcaa aatagagagt cataattaca ttagctgtgc cttcgttgtc
     4081 attcaatata ataattgact catgaccttt aagaggttca tcacccggtg gaggcagttc
     4141 accatcaggg aaataccata ttttcttgcc gttattgagt tctattttaa tcaacctcct
     4201 taaaatgttt ttcatatacc cttgacagga attagctggg cttgataaat aatttattgt
     4261 ctatttgtaa aaacgttatt acaaccttgg tcagacttgc tttggagttt taataacatc
     4321 ttaacaattc ctgcactact tgcaggggtt atgttttaga ttgaaagtca gcaaagcaaa
     4381 tgcatgtcct gcaaacacac tacctaatag aggtaggaaa accttatttt acaaattccg
     4441 gttcaagaat tacattatca taaatcttaa attctttgtt atctattctc tttataataa
     4501 tttcgagtgc tatttcacca attttttctg caggctgtat cattgaagga agtcttatac
     4561 tcattaatcc tgtctcatcg gttgcatcaa aacatactac ataaacatct tcaggaactc
     4621 tcaagccttt ctcccttatt gcttttacag caccataagc tatatagtta ttagcagcaa
     4681 gtagtgcttg aggcttcttg tttagttcca acatcttttt cattgttttg taaccgcttt
     4741 caattttaaa atctccgatc ccaataaagt cattattcac tttcacacca ctgtcattca
     4801 ttgctttttt ccagccatcc aatctttcaa tggcagatgt ttcctggagt ttaccggtta
     4861 taatggcaat atctttataa cctgaactta taagatactt tgtcagatca taactggctt
     4921 tttcattatc tatagtcaca aaattatagt ttctttttac ttgcggaagg ttatcaacaa
     4981 aaactgcagg aatgctacca ttaagtattt tgtcaaaata ttcaccatcc ctgcatactg
     5041 ttgcaacaat aattccatca acctgctttt gagcaagcat atttaaatac tctttctcca
     5101 ttgtttcatc ataatcactg ttgcacagta taacaaggta tccattattt tttgcaactg
     5161 cctctgcccc tttaatcaat agcgcaaaga actcatttga tatatcaggc aatatcacac
     5221 ctatagtcat gctgcttcta cttcttaagc tgcgtgctat ctcattgggt tgataattaa
     5281 actcttttat ggcatttaaa actttttccc gggtttcatc gcttactctg tctttcccgt
     5341 taataaccct tgatacggtt gatactgata tatttagatg ttttgctatg tctacaagtt
     5401 ttgtgttcat atctgctccc ttttgtaata acgttttcct taatattatt atatatgcta
     5461 tattgcataa agtcaattta tttaatatat agagggtgca tattttatta gtaagtcacc
     5521 caaaatcctg aagattctgt tatcaaaaaa tcacaatgtg ttcttgtatt gtttattatt
     5581 ggttttactt ttccattact atcattatgt aaccctttac ctgtggaaga attacatcag
     5641 tataattttt gttctgtatc atttcaatgg gtgtcttgca tggtgctata taggcttttt
     5701 tatactcacc ttttaccgat acaactgcac cttcaggaat aatctggtgt tcttcaatta
     5761 tatccatatt gcctcttact tcggggaaag ttgctttcac atgcagctga acagtatatt
     5821 cctttttagt aagagtaaca cgagaggtag aaggtatatc tttgcatttt aatgatggtt
     5881 caggtaaaag cttttccagg caatacttta caatagcttt atgtgaaagc attgcacttt
     5941 cataataagc attaaagacc ttgaaacata tatgataaat atttccactt ccagctacag
     6001 cagcatgccc tgttgctttt tcaggcggag tatagtaata gccgtggaat ccatcccagt
     6061 gtctgttgaa atatggtttt atgtattcag caaggatttc agtttcatta ttttctgcag
     6121 gtcctctagt ctctcctaat atttcaatct ctttaccagc tttcatcaat attccatggc
     6181 tgtacattgc ccatcgcata tctccgattc cacagtcatg atcttttacc atacggaaat
     6241 atgatgaatt ggaagtatca agcccgtcat attcgaattt ccattgagtc atcgcaaaac
     6301 cggttttgtc agggtttaag ccaccttctc cagtactgag tatcccctta ttatcagata
     6361 aatgcttttc aagtttctcc tttaaggccg gtgttacttt catattatca ggaagtatta
     6421 atatctgata tttggtaagg tccatatctt catttacaat atcgaaacca tattttaatt
     6481 cgccaagcat tcttgagaga cctttatatg tattatccag gaatccgctt gagtcggtca
     6541 gtacacctat atctgctata taagaggcat cttccgtcca aggctcatac ttttcaatat
     6601 cactataaat ctcacccaca accttatata cttctggttc caggttttct gcaggatgca
     6661 tgtgatctcc tatagaaaca tccaagccat tggaaattgc atcccacata tcatactcaa
     6721 gagatgcctt gctcttaaga cctccaaaat cgccccagct tctttgaaat cttcctgtca
     6781 tgtaaagtac tttcttttgg atgtttctag cataagcagc ctgtgctcca aaataatcat
     6841 agctccatcc gctgggaaga cattcaattt ctatatgtgt atccaaatct ttcactgagc
     6901 gataaggcat tccattaaga tataaatact tatcgtttcc tgccattttc ttaacatctc
     6961 tgctgaattg cataagaact tcttctgaat gttcacgcac atgagcatca ttaagaggat
     7021 ccaacccctg atttaccata tcttcaagac attcgttgcc atagcagggc caaaggccca
     7081 tacaatcaag gaaaaggcca tctacatcgt agttatcgag tacttccttc atcattccca
     7141 ttatatagct gcgatatccc ttgttattat aacacatgtt gcggaaaaaa ttagctgttc
     7201 ggtctccata aataacctgg ccatctttgt tcaatataca ccattctcta tgaagccttg
     7261 cttgttcatg atccaggcct acattaaaat aggctgtaac accaatattt tttttatgac
     7321 attcccttag taggtcacca aacatatcac ctttcatgcc cgggtaaggt atgcctatct
     7381 ttgtaggata ataagcaaat cccagattac acttggcaaa ggcgtttatg tattctacct
     7441 ttgcttcttt gagagtgtct gcaaaaacct ttgcatccca attttcattg aagttataaa
     7501 tgccgggcat ggtgtgaaag tctagatgaa ttgctctttt cataggtttc atgattttaa
     7561 ccccttccta ataatactta agtcagcatt attttttaac ctacatcttt cttaaaaagg
     7621 catctttctc aaataggcat cttttgtaga cgccttattt cgtccaccat tgatctgcct
     7681 atatctaatt ttccatctgc aagcatttta agaaataccg tcaacaattc attatgggct
     7741 tcacaagtat acatattacg gattgccagc agcggatgac ctgttggaaa ctccgtttca
     7801 tcaagaaatt cctttttaaa gactactctt tctgcatggg gtatcccaga tataatacac
     7861 cagcctttaa aaaatttttg aagcaattgc ttatctttta gtggttcgga tatttccatc
     7921 aaatattcca gcatggcact tttcccttca ccttcaaagg gcgatataag tgtgcattct
     7981 tgattgaaac gatatgggtt aatctctaat tcagcaggtt ttcctttctc aaatgtgata
     8041 agcggtatat atccgtaata ccatggactt ttatgatcct catcaccaaa tttggaaaac
     8101 aaaaaattac ctatgctgta cactattggg gcattattgt aggtctcaaa tccttgcaca
     8161 cagtggggat gcatacctat aaccgcatca gctcctatat ccacaaaagt gcggtaaaga
     8221 gctacaacac gtggactggg aatcggatta tactcatttc cgccatgcat tatgatcatt
     8281 ataaaatcag cattggcttt tgcttctttt atagtatgta taattctgta cagatcaaaa
     8341 cctgcagctc cagctttttt tgcttctgca attccaaatt cattttcaca tgttgctata
     8401 actgccagtt tacagttgtt tgcttcataa taccaagggg tataactgtc atcaagattc
     8461 ttccctgctc ctacataagc aattccctct tttttaaggg atcttagcgt agaaaataca
     8521 ccttcttcac catagtcacc aacatgattg tttgcaagta tggcacagtc aaagtgcccc
     8581 tcttttagaa atgctagatt ttcagggttt gcctttaatg gcggacctga tttgtgaata
     8641 ggtgtaccac ctttaatcac tgcattttca aggttgatta agcgtacatc agctttatct
     8701 aaaattggct gaatcttact taaagcagtt ttagaatact gagcatccat aagttctgca
     8761 agattacgca tacatatatc ccctgcaact ccaacgatta gtttgttatt cctgcctgat
     8821 atacctgaca tgacttgccc tcccttatga ttttgtataa tatataatca tcttttctag
     8881 ttctattttc ctcatattcc tatattccaa gtaattttgc agcattttct cttgctactt
     8941 taccgaatgt ttcctgactg attttatttg tatcacgcca ttttaccaga aggtccgtga
     9001 gcgggaccgg catgtcagcg aaacacatat cagttccaaa gaataacctg tcctggaatt
     9061 cttccagaaa cttaggacca tattctggat cacgagataa tgcattgtat gcagtgtagt
     9121 cggaaaggtc accgtaaagg ttttcatatc tacggaaaag tttcggaaca acaccttctt
     9181 ctttaattgg accgccaggt aatcatccaa cctgaccacc ttttaaatca aaaattattg
     9241 atcgttctcc cggagtttct aatcttccga tttctgacca gaatacaggt ccgtgtccaa
     9301 agattattaa attagggaac ctttgcaggg tatgttctaa tccggaacat ttaacataac
     9361 ctctccaatt cctttgcacc ctttgtcttt ataataagaa agcagcttat ccagcggtgc
     9421 ttctgctgaa tttgttaaag cacgggggtc aatattacag aaagggatca atctgtcagg
     9481 gtattttcca gccatgtcta aaatatcctc attggtttgg ggtaaatata tttccgggct
     9541 gacaatggga agcagtactc ccttttcaat acctaactca tcgtatcttt ttagtaattc
     9601 ttcggcagta caaaactgta ctatgaaagg tacaggcttt ctgtatgcgt gggcatgaat
     9661 atctataaac attttaagct ccattcctgc tatgcggctc cattcctgcg atgcgtaaag
     9721 cgaactcgcg catgcaaatt attttttcag aatatttttt atcttgtttt ccattctttc
     9781 agcatcataa ggtataagga gaccggcttg agcgtgaaac tctacatttt caccaccttt
     9841 aattattttt atatttccct tttctttttc agcagtataa cctttatgtt ccgctgttgc
     9901 aggcaataca ataccaagag cgtcttcatc tttagtcctc gatatccatc gcaacccgta
     9961 ttctagctca gaaggtctgt gtgatatata actggcatat ccatcaggat gtgcaagcat
    10021 actgtgagca ttgccttctt catcagcttt atagttgatt gtaaataaga tttccggatc
    10081 aaacaccagg tcaggtgtta atgtgtgatg aagttcagga tttaccttaa gagcttctag
    10141 aaagtcaacc agttttataa tatctggatt agacttaatg tgagtgggta tatctgcatg
    10201 tactcttaca tccttaggat tgcagtcggc actgtaaatc agtacaccat tgtcaatcgg
    10261 tctgaaatta acatgcatca ggtacataag ttccatatca gtttttttca ggtttgtaat
    10321 tttcatagaa acattgaata ccgaagaatt ctcgtatatc tttattaaag gttctgcaat
    10381 ataattatag ttgaatgcaa ccatgtgatg atattgaccg ccaatagata tgtaatgccc
    10441 tttttcatca ctacctgctt ctagatatgc tttttgatat ggagcatttg gtaattctcc
    10501 gtgttgggga tgtttatctt tttccgaagg cacccccatg gctgttgctc cacaatgtag
    10561 cagaaatcct ccatatgtct ttaaaaattc acttgttgca acaggttcgg aaaacattga
    10621 cttcatcgca agttcacgtc catgaaaatt gcagctccat acttgctggc cttggtacgg
    10681 taaaattaca acgttaccgt agttattttt aattctcaat ccatggacac cgctatcgaa
    10741 aagaaacgtt gaagcacaaa taccctggtt agcaacaagt actttttcct tttcactaaa
    10801 aaaactttta tcaaggctta taatagttga ccaatgcata aaacccaccc ctacgaatta
    10861 aatttcttag tgctagtctt ggttttcttt aaaaatatct agaatagctt ttatgtaata
    10921 acgtttttac acattaataa tatactgttt attcttaaaa gtcaatagtt atagttgtaa
    10981 taaagaatat acaaaatcag tgtttgagca taataaacac aactgttata tgatcaggct
    11041 atttcgcaag tttgattttg cccgtagttg aatttaaaag ttctaagcac cgtaacgaca
    11101 acccctgata aatatttctt cagatttgat aaatgcacct ttatattaat tcgtgcagtt
    11161 tttcaaaagt ccatacatca ttctcataga ccggtatttt aggagcattt ttattcaata
    11221 tactccctcc tgccgtcatt gaacagctta tacggttttt tttcattcaa atccatagta
    11281 acagtgtaag ctcgttcagg tgcatcatct ttcgtatcaa tagcacgctg tacagccccc
    11341 ataaattcgt tattgctcat atcaaatata ttacttttat catttgcaat ggaaatatca
    11401 aacggaatac tttttgttct tactacactt ttaaaaaaca tatttacagc agtagacgca
    11461 tcaagtccaa aaatatcaaa aagcttaaga gcttcctctt ttgcggctgc atcaacagta
    11521 agattaattc tcgcagttgc cataaccaat acctccttat aaattgttca atattattat
    11581 attatattat gctcactttg tcaacagtta tgcacaaaaa tgcgcataac tgttgacaaa
    11641 cctcagttgg caaaagtggt atatacataa attcttcaca tggtatgtac gattttctga
    11701 tgaataagaa aaggaaacgg tgaaatagaa acaaaagaac cgtcccccag tttcacatct
    11761 ccagtttcat gcataagggg tgcataagtc caatatggac atttagatca tattgcagta
    11821 ttatataaat ataacgaggc gataaatgtc cctacctcta tatgtcttgt ataaaagcaa
    11881 gaattaaatt acatttttat taaggagtga aaattattaa tatgtcttat gaaaaatcca
    11941 taagtgaagc taagcaagaa ttcacaaaca tagtcagaga ggttggtatg ggttatgaag
    12001 taatagtgtc caatcacaaa agccccaaaa gaggccgagt ttcaataata tcaactgatg
    12061 tttatgaaga tattttaaag cagggcttca agtttaatcc cattattgaa aaagatgaag
    12121 aaagtaaagg atttaccatt tttttaaatg aattacttat acacggggat ggtcctacaa
    12181 catatgaagc tttgtttgac ttggcaagca atgttatgga ctacgcagat gattatatta
    12241 aacggtttag ttttttcagg caaattgata acagaaagag ccattatcca tatcttcgaa
    12301 gaataaataa atgctccgat ataaatgaag taatggaggt aattgctgaa tgccatatag
    12361 atttacaaca ggcgatatta aggaaatagc acatcgcctt gggctgcata aggtaaatga
    12421 aaaaaaatgg aatggcattg atataaatgg taacttcctt caaacgtata ttcacgatca
    12481 tggtgacgga gtagagatat taaccggaac tgcaaaacaa cactccatgc aaatgagttt
    12541 taaagattta gatgatatgt acaattttct gatgaataag aaaaggaaac ggtgaaatag
    12601 aaacaaaatg accgtctccc tttctcgctc ttcatcacaa ttgattaaaa cccggccatc
    12661 tacctttagt accactatgg caatttgctt catcagtata ctcttacctc aaatatttnn
    12721 nnnnnnnnca tatgggttgc cccaggtttt aataacctca attctaatac cataagcttt
    12781 tgttccgggt gctagagtat gtttaacata tcttttaata ttgttttctt catgcaccaa
    12841 tatccgtgat ggctgcccat ctgcatttag tgcatatatt ttgtattctt ttacaagttc
    12901 taccggcatt gagcaatcgt tttcactcca aatacctggt cttaggttat ttctttctgt
    12961 attaaggtct gaattgaaaa acaaaattgc ttcatttata cttttttctt ctttaaattg
    13021 cagatcaagg tgctgacttt tatcagcatt gattctatca gatatccata gattaggaag
    13081 taaatatgga cggttataac cgtttataat attgtttggg ctatatatgc tatttatatt
    13141 atctgaattt gattcaccta tgccaggtcc ggttaaattt gcatcagacc attcagtgtt
    13201 tggaacagtt gtattaattt tatatttgaa gcatgggtta aaagtcgttc gcattcctga
    13261 atgcaaacag caaaatatac ccgtacatga tacttctgat tcatatatct taacgttttc
    13321 gttaggtaat atttttgcaa ccattgttcc tgtatatttt aagttaatag gaatatccat
    13381 ccagattgca gttcctttgt ttatagatat ggtttgttgc tttattagct tttctggtaa
    13441 aaaattctgt ggtttctccg gaatataaag tattactcca agatccgtat cttcatttac
    13501 gtctacgaga atttgaaatg catccattct gccttttgca ggaaatgtta tatacatgcc
    13561 cttgttaagg tcaagctttt tagaaatcgg gcttaggtat ttataatcaa tttgccggtc
    13621 ggatgtagct tgttcagata tagattggtt agcaattttg gcgtaagtaa gttgaataag
    13681 agaatttgcc ggagtcgtat tttcaggcac tctcaatgat gaagctgaca ctaaagcctc
    13741 cctggctatg tccagaggat cgctgttttt cattcctatt atatagcagt cttcttttaa
    13801 aagtcgattt ttgtatgctt caagcatggt tttgttttca taaattcctc taggatttat
    13861 tttatgattt ccttctgaat ttgcctcatt gctaacacaa aaagatgcag ctgtgccgat
    13921 tgcatgaccc atcattgcag ttgtattcat tactcttgtt gaggcaaaag cgatatgggt
    13981 tgcactaata tttctgcctg caaatagaag attgttaata ttctttgaat acattgatct
    14041 gaaaggtatt gtataagcac ctacctttat ctgcttacat cctggttctt ttgaatatat
    14101 tccttcaggt ggatgagtgt ctatgctcca gccgccatag cataccgcat cttcaaatat
    14161 ccgctgggat tcaagatctc tttgatttag aacataatcc ccaagcaatc tcctggattc
    14221 acgcttgccg ggaatatatg atatccattc aagagtataa ttatctgctt caaattcacc
    14281 actatttttt atgtaatccc atataccaaa tacaagcttt tgtaatttat ctttgattgc
    14341 ttcattatca ctaatagtat tttgaatgcc accatattca aaccaccaat aatcgcaacc
    14401 cgagcctttt acactgacaa tacggttacc aattgaaagt atctgcttta ttttttccca
    14461 atcatatgca aaatccggtt ttacatagcg aattttttta tctgttttct ttgtataaaa
    14521 aaatatactg cttccaagtg tataattgtc agctttttca ggtgccatat tttcgttata
    14581 ttcatttttg ccttctcttc ccatcatata ctctgctcct gccaaataac ctaaagtacc
    14641 atcacctgta cagtcagcaa atattggaga atagaaagta acttccagtt ccgatcccag
    14701 ttgccatcct gtgcacgaaa ggatctctct tcggtattca tttgattgat ttgcacatga
    14761 ttggcttgca accagctggt cttcatatat ctggcttgta tctgcctggt cttcatatac
    14821 cttatcatta ttcatagtta ttttatatac gctggtattt aggaaaagtt cgatattctt
    14881 ttcccttttt acaaaatcaa gaagaaccat gtcccataaa taaggattac cttcagggtt
    14941 tctatataga ttttccagtt tcatctcacc tataattcct gtttcctcat aaaaatggtt
    15001 tcctcctcct atagcaccaa gagaccatac tcttatttca cttgaagagt tcccacctag
    15061 aacaggtctt tcatgtacaa gagcaactct taagccattc ctggcagccg atattgctgc
    15121 gcaagcaccc gccagccctc ccccaacaac aacaaaatcc ttataaactt ctttcatatt
    15181 gcttataccc ccccaatatt ttctttatac atacataact ttcatatata actttcaaag
    15241 ataacacaca agtataactc ataaaagaaa ttacttaact taaattatga tatatgtaaa
    15301 agtgctgata acatgagcat accaatcttg ttcaataaaa tcattgaatt acttgcaatt
    15361 aatcttgaaa attctgcatt aatttttctg ctgcacttta aagtacattt tattaatgca
    15421 ccctgcactt aaataaagat atgcttaata tacataatat gttatactta atactactat
    15481 accaatttgg aaatttagca aagtagtatt aatatattat gttaagtata tacggagtaa
    15541 tattatggat aagttgaaat atgcagggtt actggaatac ttggatttta tatcaaaaac
    15601 atataatatg gaaatatgta taaatgattt caccggcttt acaatgcttg agcctgatct
    15661 tatgtctgta ctccagccat atatgattca taagaataaa ttctgcatgt atctaaaatc
    15721 gaataaccaa ctatggcaaa agtgcataaa aaataaaaaa aagattcttg ataaatgtaa
    15781 taaagaaaaa cagattttta aaggcatatg cccatctggt gttgaagagt atattgtccc
    15841 tatactctat gataatatgg ctattggatt tatcagcgta ggaatataca gttcgaataa
    15901 gagaatctcg gaatatttat taagaaagaa gtgccttaga tataacctgg attttgaaaa
    15961 agctcttgat ttatataact cagaatatag cagtagctgc catagtgacc aattaatacg
    16021 gtctgttttc agcatcctgg tacaatactt tcaagagtta tatataagac atataaactc
    16081 aatcacttat aagcataaac agcaaaataa gcaacaccaa ttgtctcctc aaa
//
LOCUS       DAOSDD010000092        15770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1498_length_15770_cov_5.182119, whole genome shotgun sequence.
ACCESSION   DAOSDD010000092 DAOSDD010000000
VERSION     DAOSDD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15770
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1498_length_15770_cov_5.182119"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK733_09615"
     CDS             complement(<1..916)
                     /locus_tag="PK733_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ10837.1"
                     /translation="MKWLQSFRMAFKSIAGNKIRATLTMLGIIIGVGAVIGMVSLIEG
                     ATRQIKDTLQGMGTNLISINVLGRGSTRTATAEEIIEFAETNSDVIEGVAPLISGTAT
                     VKYGNKNLSTNLEGTNEAYRTVRNTDIQEGRFFNALDIERRQKVVLIGTYISQELFGN
                     TDPVGQNVKINGEIFKVIGILETKSNSTAQSGDDKVIIPYTTASRLQRNSQIRTYYVQ
                     GKTPETVDRAMQVLEAFLFKKFNSTSAYRVFNQADMLESISETTKMLTMMLGGIAGIS
                     LLVGGIGIMNIMLVTVSERTREIGIRKAI"
     gene            complement(917..1594)
                     /locus_tag="PK733_09620"
     CDS             complement(917..1594)
                     /locus_tag="PK733_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10838.1"
                     /translation="MIRIENMYKIYRMGTNDVYALNGISLHIKPKEFVAIVGPSGSGK
                     STLMNMIGCLDTPTSGKYFLDGKEVSKLKDDALADIRNKKVGFVFQTFNLLPKLTALE
                     NVELPLVYMGLGRKEIHKLAVEALEKVGLGDRFHHKPSELSGGQQQRVAIARALSSRP
                     SLILADEPTGALDSKSGEEIMQMIHELHKEGKTIVLITHENDIANQAERIIRIHDGIV
                     VEESGVA"
     gene            complement(1628..3586)
                     /locus_tag="PK733_09625"
     CDS             complement(1628..3586)
                     /locus_tag="PK733_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ10839.1"
                     /translation="MKKIFSSKILKNKIYKRILIVLITAGICLTGYTIYDRIAAKAST
                     SKNLVELTAWAARGNIDVKLSGTGTVQPVSRCDIVPLVKGKILNASFEQGMEVKKGDL
                     LYQIDDSDLHNSIEKARNSIEKQKLNNKSTDNSIKNLIVLAPGTGWLKNFSIKEGDQI
                     GNANSKIGDIVNDKKFTVQAPFYASQVHKIKSGQKAQLVLAKYMTYIEGEVTDISRNL
                     KLAQDGSVLCDVEIIADNPGTLIEGMQVSAIIEVDNEEIESAGESGIKCYEEYPLYAE
                     TTGEVGKILVKNDEWVEKGQKIMELENENLNVTSQRNALDLKDLELALALQIKQLDDY
                     NILSPINGTIIKIDYKAGDTINAANSNTVLMTIVDTSKMKFTMDVDELDISKIAEGQK
                     VSIASDALPETTLEGKVTAIPVEGKSQNGVTTYPVEITIDQPGNLKSGMNINAEIFVE
                     SKQNVLYVPMAAVNKTGGRTFVYVKDDSAKQQNGNTQQSASPWQNGSAQQNAGSGQQR
                     PSRQDAMNGDRTQQYSGNWGQGNFQGRMQEGAQQTPGEGTQDFQNRFANARRNTAGSS
                     AAAVNSLEGRTIREVVVGINNEDYIEIVSGLNEGEIVILPTVATNNNSSNTPSGIFGQ
                     PGMGGFRIQGGGGAGGAVTRTTGGFNGR"
     gene            complement(3769..5292)
                     /locus_tag="PK733_09630"
     CDS             complement(3769..5292)
                     /locus_tag="PK733_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ10840.1"
                     /translation="MMFSIWYSIRFKLFAAIGGIILFFVAFSWLLNSTFLEKYYYSVR
                     KQYLIENYKYVYDIYNGDLEEVAFDFEMLERTKSLHIFIIDKNYNIKYASIREIRYNR
                     RRQPGWNVLPSEYSMPEFLIKQRIERLSKGEVLVENRRDDRLQTNFISLFSSLKNGDF
                     IILNIPITSIKESVNISNNFFMFTGVITIFIGSIFVILITGKFTKPILELNEIAQGMS
                     QLDFSRRYPVKNRDEIGKLGESINSLSEQLEKSISELTRANQKLREDIEKERKIDEMR
                     KEFISNVSHELKTPIALIQGYAEGLKVNVNEDPENKDFYCNVIIDESMKMNKLVKQLL
                     ELSQMESGKTSLERVDFSINDLIEQVLRKNELIFKEKNINVIIKMDRDIYVNADFEKT
                     EQVLMNYLSNALNHVDERKIVKISAKLPDDISDNHKKGKIIISVFNSGKLIPGKDIKK
                     IWTSFYKIDKARTRAYGGTGLGLSIVKAIQEAHGNRYGVINLEKEGGVEFWFELDLR"
     gene            complement(5289..5966)
                     /locus_tag="PK733_09635"
     CDS             complement(5289..5966)
                     /locus_tag="PK733_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ10841.1"
                     /translation="MSGVKILLVDDELRMRKLVSDFLKRNGYAVSEAEDGKRALELFY
                     STPDIKLVILDVMMPELDGWSVCSEIRKSSSVPVIILTARSEEADELFGFDLGADEYI
                     TKPFSPMILMARVQALLRRTVNGTKNIKSFEGLEIDETGREVKVDGESIDLSPKEFEL
                     LLYLANNQGIALSRDQILNSVWDYDYFGDERTVDTHIKKLRLKLGEKGDYIQTIRGLG
                     YKFEVKK"
     gene            complement(6146..7894)
                     /locus_tag="PK733_09640"
     CDS             complement(6146..7894)
                     /locus_tag="PK733_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10842.1"
                     /translation="MPATKTKKESKSIGLHNKSILHNLLFVILMIFTFTMSSTISSIS
                     SAAGISGANTGEDSSGFKFNMTYIHFNDKNSYLKYVENTKDSINEVSPNYLSLKYDGT
                     LQISSTLDKGFIDEMHKRGIKVVPFLSNNWDRTLGRFAMGKRYKLSEQIAEAIEKYNL
                     DGVNIDIEDLTEKDRDRHTDFIRMLRKKIPADKVIAVSVASNPKGITTGWHGSYDYEG
                     LAEYCDYLMIMTYDEHYGGGPSGPVASIGFVERSIEYALKKVPKEKIVLGIPFYGRMW
                     KNGGGFNGQGVSLKEVSSLIARYGGRVSFSNAYLSPKAVVTIKSDDDKPYINGKKLEA
                     GTYTIWYENEDSLKHKLSLVKKYGIKGTGSWSLGQEPSSIWNYYSLWLNGHYYKDVQG
                     HWAQNPIIFALNNGWITTKTVKDSGVFAPDKPLTRAEAAFALVRSLELDKDRKQESKI
                     TFSDISNHWAKNEIDIAAQHRIITGRGDGTFGPDDAVSREEMSVMLDRAISGLSGRST
                     VLASRGMGSGSNMNISYVDVSRELCTWSYDSIIKMSQQGIITGTPDGKFNPKSKISRA
                     ETAALLFKVRGHSSLE"
     gene            complement(8037..9206)
                     /locus_tag="PK733_09645"
     CDS             complement(8037..9206)
                     /locus_tag="PK733_09645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ10843.1"
                     /translation="MENFNFCSPTKIIFGKATEDCAGQETAKYGKRVLLHYGGGSIKK
                     TGLYDKVVKSLKEAGVDYIELSGVEPNPRLSLVKEGIKICRTENIDFILAVGGGSVID
                     SAKAISIGVPYDGDVWDFYSGKAEPEKSLGLGVILTISAAGSEASKSSVITNEDGWYK
                     RGLGFDIVRPEFAIMNPELTFTVSAYQTACGSVDIMAHIMERYFTNTLNVELTDRLCE
                     GALKTVINNTPIALKEPGNYNARAEIMWTGTLAHNDLLGTGRIGDWASHQIEHELSAI
                     YDIAHGAGLAIVFPAWMKYVFKSNTKLFAQFANRVWNVDYSFESPEQTALEGINRMDK
                     FFREMGLPTTLRDAGIPEDRLEEMAQKCRLPNGDSVGNVVKLTRDDVLNIYMLAK"
     gene            complement(9292..10374)
                     /locus_tag="PK733_09650"
     CDS             complement(9292..10374)
                     /locus_tag="PK733_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ10844.1"
                     /translation="MKIFDNKMTELKFFVESGRRPDFNNFLKLLKGEKPERPTLFELF
                     LNNNLYEELANEEFGDTSDKINRYTIIIKAFANAGYDYATVYGSDITFETARHAHGNA
                     KTISLNEGAVITDLQSFKQYRWPDPDKYDYSHLKSLETVLPEGMKLMVMGPGGVLENV
                     IALVGYEQLCYMIMDDPELVAAVFEEVGRRLVRYYEICAPYDTVGMLMANDDWGFKGQ
                     TMLSVEDMRRFVFPWHKRIVEVIHAAGKPAILHSCGNLSMIMDDIIDDMKFDAKHSYE
                     DNIQPVEEVYERWRGRIAILGGIDVDFICRSTPEEVYDRSCAMLERTADRCGYALGSG
                     NSIPYYVPEENYFAMIAAAFTYMSKK"
     gene            10785..11657
                     /locus_tag="PK733_09655"
     CDS             10785..11657
                     /locus_tag="PK733_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10845.1"
                     /translation="MRTLHENINMPDSGFPIKAFIRSYRSESNIFVHPHWHDVIEILY
                     IIEGKAVQQINNRIFEANKNELVVIAGGQVHSTYTQKENKNEILVLQFSPDLFSSEDH
                     LFPENKLVKQFISSIDFPNSISNKTVAGTEAINCIKDIYEELEYKIYGFEIFVKACIL
                     KFIGIMVRNFGHMKRKREDVVKLNSTKKILNNTFKLIDDNFSSDIPLEKAAKASNLSI
                     PHFCRLFKSSTGMTFTEYLLFYRINRAEEMLRSDLTITEIAHECGFSSSASFSRAFRK
                     SRALSPTEFRKKYL"
     gene            complement(11741..12982)
                     /locus_tag="PK733_09660"
     CDS             complement(11741..12982)
                     /locus_tag="PK733_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOJ10846.1"
                     /translation="MKRIISMLISIIIVACLFMGCVGSAGNGNNRIGGSKQASYDLNK
                     VDPSLTDGNELFAFDIFKELNTEDADKNIFISPLSISTALAMTYNGAGSTTKEAMGEA
                     LRYRGIELENLNTGYKNLLAYLNNVDPKVQLNISNSIWFRKGEEIKQDFLSVNKDNFN
                     AYITEMDFQDSKAADTINGWISKATKGKIDKMIEPPISHDVIMYLINAIYFKGQWTKQ
                     FDKKLTFTGTFNTEDGQKKEVQMMHRKGKIEYGEVEKSKIVRLPYGNEKTAMYCILPE
                     TGIKINDFIRQLSVEKWIELKDSLSKVDDVVLQLPKFKMEYGIKNLNDSLTALGMGEA
                     FSDSADFSGIRDDICISRVLHKAVIEVNEEGSEAAGATVVEMKETAAVEPVTFIGDRP
                     FIFIIADDETGSILFMGKFME"
     gene            complement(13144..15165)
                     /locus_tag="PK733_09665"
     CDS             complement(13144..15165)
                     /locus_tag="PK733_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ10847.1"
                     /translation="MDIYVSTMGNDNWTGLLPNPDNTGTDGPVATIERARDIIGEIKY
                     TGRFDGPVNVWIRGGRYAVEKPVTFKPEDSAPVCYKAYPGEQPVIHGSKRITEWSIGT
                     VADSSICCWIADIPEVREGKWYFRQLFVNDQRRQRAKFPKDGFYRMESVPGLTVEPWY
                     RYWDGQDAFVAADGDFKEWQNINDIELVTFHKWIEERIPIKSYDEVSRVVTLSRKSTM
                     ALSDDFEKKYPRYYIENIFETLSEPGEWYLDRKLGKLYYIPFPGEEPDTAEVFAPCTT
                     QLLKIEGCPDVEKYVEFLSFEDLVFEHGDWNLDNGPSVQGAHTIPGVITMKGARCCAI
                     KNCTIRHAGFYGIEIADGCMGNRITGNKIYDMGAGGIKLGGSDAKGPGTGLTGNNVIT
                     SNHIYSAGQVFYSSCGILSMNSFGNDISHNHIHDLYYSGISCGWVWGYSESVSKNNRI
                     EKNYIHDIGHGLLSDMGGIYMLGVQPGTVVWGNVVHDIEKYCYGGWGIYTDEGSSHIL
                     IENNICYRTGSQCFHQHYGRENILRNNIFAFGREGNAALSTMENHLAFTIERNIFVTD
                     GPPVFLSSSTDKFKKQNFISDLNLFWSVKGNIAAAGGWEYLSLEDGMLPENTIDMDEW
                     KRMGYDNHSICADPKFADIMNFIFVLEPDSPAFSLGFKQIDISDVGPLT"
     gene            complement(15201..>15770)
                     /locus_tag="PK733_09670"
     CDS             complement(15201..>15770)
                     /locus_tag="PK733_09670"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10848.1"
                     /translation="APGDRHITEFFTEYFPGGKYYSYTLGYDAYSFEDTIALGDEIHN
                     EAMKKAFSSESLDDDFLRHISGEQEQLTSIIDSMERDSRDVYYMNVQNNGAIPNLPPW
                     AIIEMPVLATAAGPKPIYLDDFPDVLAGFTSRFLSVIEIAVDAALEGSRKLMEEAILA
                     GGYISDRKAVAKMADELIKAQIRYLPQFE"
BASE COUNT         4449 a         3497 c         2464 g         5360 t
ORIGIN      
        1 caattgcttt ccttatacct atctctctgg ttctttcact tactgtaacc agcataatat
       61 tcataatgcc tatacctcct acaaggaggg aaatacctgc tattccacca agcatcattg
      121 taagcatctt tgttgtttca cttatactct ccagcatatc cgcctggttg aatactctat
      181 aggcactggt actattgaat tttttaaaaa ggaaagcctc caatacctgc atagcccggt
      241 caactgtttc aggtgtcttg ccctgtacat aatatgtacg tatttgtgaa tttctctgca
      301 atctactggc tgttgtatat ggaattatta ctttatcatc accagattgt gcggtactat
      361 ttgattttgt ttctaaaatt cctataacct tgaatatttc accgtttatt tttacatttt
      421 gtccaacagg gtcagtattc ccaaacaatt cctgggatat ataggtccca attaaaacaa
      481 ctttttgtct tctttcgata tccaaagcgt taaaaaatct gccttcctga atatcagtgt
      541 ttctaactgt ccggtaagct tcatttgtac cttcaagatt tgtagaaagg ttcttattgc
      601 catattttac tgttgctgta ccacttatta gaggggcaac gccctcaatg acatcgctgt
      661 ttgtttcagc aaactcaata atttcttctg cagttgcagt tcgtgtactt cccctgccta
      721 acacattaat actgatcaga ttagttccca tgccctgcag ggtgtctttt atctgtcttg
      781 ttgcaccttc tataaggctt accattccta ttaccgcacc aactccaata ataataccaa
      841 gcattgtaag ggttgcacgt atcttgtttc ctgctatgct tttaaaggcc atccgaaatg
      901 attgaagcca cttcatctat gccaccccgc tttcttcaac cactattcca tcatgtattc
      961 ttataattct ttcagcctga tttgctatat cattttcatg agtaataagt acaatggttt
     1021 ttccctcttt atggagttca tgtatcattt gcataatttc ctcaccggat tttgaatcaa
     1081 gagcccctgt aggttcatca gcaagaatga gagatggtct gctggataat gcgcgggcta
     1141 ttgctactct ctgctgctgt cccccggaaa gctccgatgg tttatggtga aatcggtctc
     1201 caagtcctac cttttcaagg gcttctaccg ccagcttatg tatctctttt cttcccaggc
     1261 ccatataaac cagaggaagc tcgacatttt caagggcagt tagtttcgga agaagattga
     1321 atgtctgaaa cacaaaacct acctttttat tgcgtatatc agccagggca tcatctttta
     1381 gtttacttac ttctttacca tccagaaaat attttcctga tgtaggagta tcaagacatc
     1441 ctatcatatt cataagcgta gattttcccg accctgatgg cccaactata gcgacaaatt
     1501 cctttggttt aatatggagt gaaatgccat ttaaagcata aacatcattt gttcccattc
     1561 tatagatttt gtacatattc tcaattctaa tcactttctc acccctgttt ctccggttta
     1621 tgctttccta gcgcccgtta aatcctccgg ttgtccttgt tacggcacct cctgcaccgc
     1681 ctcctccttg aattctaaat ccacccatac cgggttggcc aaagatgccg gatggagtgt
     1741 tgcttgaatt attatttgta gcaactgtag gaagaataac tatttctcct tcatttaggc
     1801 cgctgacaat ttcaatgtaa tcttcattat taatgcctac gaccacttct ctaattgttc
     1861 ttccttcaag gctgttaact gcagctgctg atgatcctgc cgtgttgcgt cttgcattag
     1921 caaacctatt ttgaaaatcc tgggttcctt ccccgggcgt ttgttgtgct ccttcctgca
     1981 ttcttccctg aaagtttccc tgaccccaat tgccggaata ctgctgagtt ctgtcaccat
     2041 tcatagcatc ctgacgggag ggacgttgct gaccggatcc agcgttttgc tgtgcacttc
     2101 cgttttgcca cggacttgca ctttgctgcg tatttccatt ttgctgcttt gcactatcgt
     2161 ccttaacata aacaaaggtt cttccccctg ttttattaac tgcagccata ggtacataaa
     2221 gcacattttg cttgctctct acaaatattt ctgcattgat attcataccg gacttaagat
     2281 tgccaggttg gtcaattgtt atctccacag gataagttgt aacaccgttt tgtgatttcc
     2341 cttctacagg aattgcagtg actttccctt ccaaggtagt ctcaggcaaa gcgtcggagg
     2401 ctatggaaac tttttgtccc tctgcaatct tggaaatgtc aagttcatct acatccatag
     2461 taaatttcat tttactcgta tctactattg tcattaaaac tgtattgctg ttagcagcgt
     2521 ttattgtatc gcctgcctta tagtctattt ttataattgt tccattgata ggtgaaagta
     2581 tattataatc atcaagctgc tttatctgca aagcaagagc aagttcaaga tccttaaggt
     2641 caagagcatt cctctgactt gtgacgttta agttttcatt ttccaattcc attattttct
     2701 gacctttttc aacccactca tcatttttta caagaatttt cccgacttct cctgttgtct
     2761 cagcatataa aggatactct tcataacact taataccaga ctcgccggca gattcaatct
     2821 cttcgttatc tacctctata attgcggata cctgcatacc ttcaataagt gttccaggat
     2881 tatctgcaat tatttctaca tcacaaagca ccgaaccatc ctgggcaagt tttaggttcc
     2941 tgcttatatc tgtaacttca ccttctatgt atgtcatata ttttgccagt acaagctggg
     3001 ccttttggcc tgatttaatt ttatgtactt gggatgcata aaatggagcc tgaacagtaa
     3061 attttttatc atttacaata tctcctattt tactgttggc gtttcctatt tgatcccctt
     3121 ccttaataga aaaattctta agccatcctg ttccaggagc aagtactata agatttttta
     3181 tgctgttatc ggtagatttg ttgtttaatt tctgcttttc aatgctgttt cttgcttttt
     3241 caatactgtt atgcaggtcg ctatcatcaa tttgatatag aagatctcct ttcttcactt
     3301 ccataccctg ttcaaaagaa gcattcagta tcttgccctt aaccagaggc acaatatcac
     3361 atctggagac aggctgcact gttcctgtgc ctgaaagttt aacatcaata ttgccacgtg
     3421 ctgcccaggc agtcagctca accagatttt tcgaggtgct cgccttagct gcaattctat
     3481 catatattgt atatccggta agacaaatac ctgcagtaat aagcacaata agaatcctct
     3541 tatagatttt attcttaagg atcttggaag aaaatatttt tttcaaagtc ttgtctcctc
     3601 ccttgctttc atacaactct gttttattgc ttcaatattc gtttatctcc ttctgatatt
     3661 aaaaaaacat tgtgttcatt ttgtgtccat tccgttaata ttctgttaaa taaaggggac
     3721 attcttcaac gaaaaagaaa ttctcccgct cgatagagga gtgtccccct accttagatc
     3781 taactcaaac cagaattcca ctcctccttc tttttccaag ttaattacac catatctgtt
     3841 gccatgtgcc tcctgaatag ctttaacaat tgacaggccc aggcctgtcc ctccatatgc
     3901 tcttgttcgt gctttatcaa tcttataaaa gctcgtccat atttttttaa tatcctttcc
     3961 cggaatcaat tttcctgaat taaatactga tattatgatt tttccttttt tatgattatc
     4021 agaaatatcg tctggtagtt tagcagaaat tttaactatt ttcctttcat caacatgatt
     4081 taatgcattg ctcaagtaat tcatcaatac ctgctctgtt ttttcgaaat cagcattaac
     4141 ataaatatcc ctatccattt taataatcac attaatgttt ttttctttaa atatgagttc
     4201 attctttctc aaaacctgtt caattaaatc atttatactg aaatccactc tttccaggct
     4261 ggttttacct gactccatct gagataattc caaaagctgc tttacaagct tattcatttt
     4321 catggattcg tcaattatta cattacaata gaaatcttta ttctccgggt cttcatttac
     4381 atttaccttt aaaccttctg cataaccttg aataagagca attggtgttt ttagttcatg
     4441 ggaaacgttg gatataaatt ctttcctcat ttcgtctatc ttccgttcct tctcaatatc
     4501 ttcccttagt ttctgatttg ctctcgtaag ttcagatatg gacttttcaa gctgctccga
     4561 aagagaattt atactttctc caagctttcc tatctcatct ctgtttttaa cagggtacct
     4621 tctgctaaaa tccaactgtg acattccttg ggctatttca ttcaattcaa gtatgggttt
     4681 ggtaaattta ccagtaatta aaattacgaa aatactacca ataaatattg ttattactcc
     4741 tgtaaacata aagaagttat ttgaaatatt aacactttcc ttaattgatg ttataggaat
     4801 atttaatata ataaaatcac catttttaag tgaagaaaac agactgatga aattagtctg
     4861 taatctgtca tccctgcggt tttcaactaa aacttcgccc tttgaaagcc tctctatcct
     4921 ttgctttatt agaaattcag gcatactata ctcggaaggt aatacgttcc atccaggctg
     4981 tcttcgccta ttatacctta tttcgcgtat tgaggcatac ttaatattat aatttttatc
     5041 tataataaat atatgaagac tctttgtccg ctctaacatt tcaaaatcaa aagcaacttc
     5101 ttcaaggtct ccattatata tgtcatagac atatttgtaa ttttcaatta gatattgttt
     5161 tcttacagaa taataatatt tttctaaaaa tgtactgttt agcagccacg aaaaagcaac
     5221 aaaaaaaagg attattccgc ctatggcagc aaagagttta aaccttattg aataccaaat
     5281 ggaaaacatc attttttcac ctcaaactta tatccaaggc ctcttatggt ttgtatgtaa
     5341 tcaccttttt cacccaattt tagtcttaac ttttttatat gagtgtcaac agtcctttca
     5401 tcaccgaaat aatcatagtc ccatactgaa tttaatatct gatccctgga caaagcaata
     5461 ccctggttgt ttgctaaata taaaaggagt tcaaattcct ttggacttaa atcgatgctt
     5521 tctccgtcaa cttttacctc gcgccctgtt tcgtctattt caagcccctc aaaagatttt
     5581 atatttttcg taccatttac cgttcttctc agtaaagctt gaactctggc cataagaatc
     5641 atgggactga aaggcttggt aatatattca tctgcaccaa ggtcgaaacc gaaaagttcg
     5701 tctgcctcct cacttctggc agtaagtatt attacaggaa ctgatgagga ttttcttatt
     5761 tctgagcata cagaccaacc gtcaagttcg ggcatcatta catcaagaat caccaattta
     5821 atatcagggg tactataaaa tagctctaat gctcttttcc catcctcggc ttcacttact
     5881 gcataaccat ttctttttaa aaaatccgat accaattttc tcattctcaa ttcatcatca
     5941 accaacagta tctttactcc agacatatca accaccattc cgccgccatg caggtgtgtc
     6001 cactaatcct acatttattt tttaaataat attttagctt ataaatgtgt cctttttgtg
     6061 aacaaaaaat aactgtggaa taaagagaga agtgtcaaag cagataatca aaaggggaca
     6121 ctcctcaaca tagaaggaat atctgctact caagagagga atgtcccctt accttaaaca
     6181 acaaagctgc agtttctgcc ctgctgattt tagattttgg attaaatttt ccatcgggag
     6241 taccagtaat aatcccttgc tgtgacattt ttattattga atcatatgac caggtacata
     6301 attctctgct tacatctaca tatgaaatat tcatattact tcctgaaccc atgccccggc
     6361 ttgcaagtac tgtgcttctt cctgaaaggc ctgaaattgc tctgtcaagc ataactgaca
     6421 tctcttcacg tgatactgca tcatcaggtc caaatgtacc gtcccctctt cctgttatga
     6481 ttctatgctg agcagctata tcaatttcat ttttcgccca atgatttgaa atgtcgctaa
     6541 atgtaatttt cgattcctgt tttctgtctt tatcaagttc aagagaacga accaaagcaa
     6601 aagcagcttc agctctcgtt agtggcttat cgggggcaaa tacgccgctg tcttttactg
     6661 tcttggttgt tatccatcca ttatttaatg caaatattat aggattttgt gcccaatgac
     6721 cctgcacatc cttataataa tgcccattaa gccataaact gtaatagttc cagatacttg
     6781 agggttcttg tcctaagctc cagcttcctg taccttttat tccatatttt tttacaaggc
     6841 ttagtttgtg tttcagggaa tcctcattct cataccatat tgtatatgtt cctgcttcca
     6901 gcttttttcc gtttatatat ggtttatcat catcggattt tattgtaaca actgcctttg
     6961 gtgaaagata tgcgttactg aatgaaacgc gccctccata cctggcaata aggcttgata
     7021 cttctttgag acttactccc tgtccgttga atccgcctcc attcttccac attcttccat
     7081 aaaaaggtat acccagaacg attttctctt taggtacctt tttcagagca tattctattg
     7141 aacgctcaac aaaacctata ctggctacag gacctgaagg gcctcctcca taatgctcat
     7201 cataagtcat gatcattaaa taatcacagt attctgccag accttcgtaa tcataagaac
     7261 catgccagcc ggttgttata ccctttggat tactggcaac agaaacagca ataaccttgt
     7321 ctgcaggaat tttttttctt aacatcctga taaaatcagt atgtctgtcc ctatcttttt
     7381 ctgtaagatc ttcaatatca atattaacgc cgtcaaggtt atatttttct attgcttcag
     7441 cgatttgctc agataatttg tatcttttgc ccatagcaaa ccttcccaaa gtgcgatccc
     7501 agttgttgct aagaaaaggt acgactttaa tacccctctt gtgcatctca tcaatgaagc
     7561 ccttatccag ggtagatgat atctgtaaag ttccgtcata tttcaaactt aaataatttg
     7621 gagaaacttc atttattgaa tcttttgtat tttccacata tttcaaataa gaatttttat
     7681 cattaaaatg tatatatgtc atgttaaatt taaaacctga actgtcttca ccagtatttg
     7741 cacctgatat accggcagca ctggagattg aacttatggt tgaagacatg gtaaatgtaa
     7801 aaatcattaa gataacaaat agtaaattat gtagtattga cttattatgt aatcctatgc
     7861 ttttgctttc tttttttgtt tttgttgctg gcattcatat cgaccccctc cgctattaag
     7921 attacaggat tttagttcta attgcaatgt atattattat acaatattat acagaagggg
     7981 ccgaattgat gcagcttcat ataaattaaa tgtaccctga taaaccgaaa ttttctttat
     8041 tttgcaagca tataaatatt cagtacatca tcccttgtca gttttacaac attccccaca
     8101 gaatcaccgt tgggaagtct gcacttctga gccatttcct ctagcctgtc ttcgggaatg
     8161 cctgcatctc tcaaagtggt gggaagcccc atttctctga aaaatttatc catcctgttt
     8221 attccctcta aggcagtttg ctccggcgac tcaaaagaat aatcaacatt ccaaactcta
     8281 ttggcaaact gtgcaaagag ttttgtattg ctcttaaata catatttcat ccaagcagga
     8341 aaaacaattg caagacctgc accatgagca atatcatata tggcactcaa ttcatgttct
     8401 atttgatgtg aagcccagtc tcctattctt ccggtaccaa gcaaatcgtt gtgagccagt
     8461 gtacccgtcc acataatctc agcccttgcg ttataattac caggctcttt taaagctatt
     8521 ggtgtgttat ttattactgt ttttagggcc ccttcacaca acctgtcagt aagttcaaca
     8581 ttgagtgtgt tagtaaaata cctctccatt atatgagcca taatatctac agatccgcat
     8641 gcagtctgat aagctgatac cgtaaaggta agttcgggat tcataatggc aaattcaggt
     8701 cttacaatat caaaccccaa gcccctttta taccagccat cttcatttgt aataaccgaa
     8761 cttttgctgg cttcactgcc ggcagcagaa attgtaagaa taactcctaa gccaaggctt
     8821 ttttcaggtt ctgcttttcc tgaataaaaa tcccatacat ctccatcata aggtacccct
     8881 atagaaatgg cctttgctga atcaataacg cttcctcccc caacagccaa gatgaaatca
     8941 atattttccg tgcgacatat tttaatgcct tctttaacaa ggcttaacct ggggttgggc
     9001 tcaactccgg aaagctctat gtaatcaaca cctgcttctt taagggattt cacaacttta
     9061 tcataaagac ctgttttctt tatgctgccc ccgccataat gcagaagaac cctcttgcca
     9121 tattttgctg tttcttgacc tgcacagtct tcagttgctt tgccaaatat aattttagta
     9181 ggactacaaa aattaaagtt ttccatatgc atcctccttt aaatactgta tacatatcat
     9241 atactattat tcgcaaaaat aaaagctttg ccgtactcat ggatgcattt tttacttttt
     9301 tgacatgtag gtgaacgctg ctgctatcat tgcaaaatag ttttcttccg gtacatagta
     9361 aggtatgctg tttcctgatc caagagcata accacacctg tctgcagttc tttccagcat
     9421 ggcgcaggat ctatcataaa cttcttcagg tgtacttctg catataaaat caacatctat
     9481 tccgccaagt attgctatcc gtcctctcca gcgttcatat acttcctcaa ccggctgaat
     9541 gttgtcttca taggaatgct ttgcatcaaa tttcatatca tctattatgt catccattat
     9601 catggacaag ttgccacagg aatgcaatat agccggtttt cccgcagcat gtatgacttc
     9661 cactattctt ttgtgccagg gaaatacaaa gcggcgcata tcctccacag atagcatggt
     9721 ttgcccttta aagccccaat cgtcattagc catcaacatt cctacagtat catatggagc
     9781 acagatttca taataacgca caagacgccg gcctacctct tcaaacactg cagcaacaag
     9841 ctctggatcg tccataatca tataacatag ctgctcatat cctaccagcg caattacatt
     9901 ttcaagaaca ccgccgggac ccataaccat cagtttcatt ccttcaggca aaacagtttc
     9961 aagacttttc aggtgagaat aatcatactt gtccggatct ggccatctat attgcttaaa
    10021 gctttgcaaa tcagttatca cagcaccttc attcaatgat attgttttgg cattcccatg
    10081 ggcatgtctt gcagtttcaa aggtaatatc tgaaccatat acggttgcat aatcataccc
    10141 ggcattggca aatgctttaa taatgattgt ataccggtta attttgtcac tggtatctcc
    10201 gaactcttcg tttgcaagtt cttcgtataa attgttatta agaaacaatt cgaacaaagt
    10261 cggtcgttcc ggcttttccc ctttcaatag ttttagaaaa ttattaaaat ccggtctgcg
    10321 tccgctctca acaaagaatt taagttccgt cattttgtta tcaaatattt tcatcgtaac
    10381 ctccatttcg ccgataccat cggcaacaca aataatagtg aaaattcatg aattcgttta
    10441 aataattcca tatcgcagcg tatagctaca aacaaattag cgtgcgttgc gataaattta
    10501 ggttctatat attctcaaaa agtgctatgt aacgcttata ctacatggtt ctgaggaaaa
    10561 aagtatgttt tttgttaact ttttaagaat aataatatat cgtgcaaatc tattatatat
    10621 tgtataattg attgtatcag atagattcat tcggtatttg gcatatatta tcaaaaagtt
    10681 tgccctggtt atcaatatta tagaggattg ctatgaagga ttgatataat agaagattac
    10741 tgcagaggat tatcactata taagattgtt atggaggatt cttaatgaga actttacatg
    10801 agaatataaa catgcctgac tccgggttcc ctattaaagc ctttatcagg tcttacaggt
    10861 cagaaagcaa tatttttgtt cacccgcact ggcatgatgt tattgaaatt ctgtacatta
    10921 tagaaggtaa agcagtacag cagattaaca acaggatatt cgaagcaaac aaaaatgaac
    10981 tggtagtgat tgcaggtgga caggttcatt ctacctacac acaaaaagag aacaagaatg
    11041 aaatccttgt actccagttt tctcctgatc tcttttcttc tgaagaccat ttgtttccgg
    11101 aaaataaact ggtgaagcag ttcatatcct ccatagattt ccctaattct attagcaata
    11161 aaacagttgc tggtactgaa gctatcaatt gtatcaagga tatttatgaa gagttggaat
    11221 ataaaatata tggctttgaa attttcgtca aagcttgcat tttgaagttt ataggtataa
    11281 tggtaagaaa tttcgggcat atgaaaagga aaagagagga tgttgttaaa ttaaacagca
    11341 caaaaaagat actaaataat acatttaagc ttatagatga taatttttca tctgatatcc
    11401 ctcttgaaaa ggcagcaaaa gcttccaatc taagtatacc tcatttctgc aggttattta
    11461 aaagctccac cggcatgacc tttacagaat accttctgtt ttatagaatt aaccgggccg
    11521 aagaaatgct ccgctctgat ctgactatta ctgaaattgc ccacgaatgc ggcttcagca
    11581 gttcagcttc cttcagcaga gcttttagaa aatcgagagc cttaagccca acagaattca
    11641 ggaaaaaata cttatagaaa aaaaagtata cactgcagac tttgtcccaa ggacagttca
    11701 tattcccagg agtctgctcg aaaaagcaga ctcccagcat ttattccata aatttaccca
    11761 tgaaaaggat gctcccggtt tcatcgtcag ctatgatgaa tataaatggc ctgtcgccta
    11821 taaatgtaac cggttcaact gctgctgttt ccttcatttc aaccacagtt gcacctgcag
    11881 cttcgcttcc ttcttcgtta acttcaataa ccgccttgtg taatactctg cttatacata
    11941 tatcatccct tatgccggaa aaatcagcag aatcgctgaa agcttctccc atgccaagcg
    12001 ctgttaagct atcattcaaa ttctttatac catactccat cttaaatttt ggtaattgaa
    12061 gtacaacatc atctactttt gaaagactgt cttttagctc tatccatttt tcaacactta
    12121 actgccggat aaaatcgttt atttttatgc ctgtttcagg taatatgcaa tacatggctg
    12181 tcttttcatt gccgtagggt aatcttacta tcttcgattt ttcaacctcg ccatactcta
    12241 ttttaccttt tctgtgcatc atttggactt cctttttttg cccgtcttct gtattgaaag
    12301 tcccggtaaa ggtaagtttc ttgtcaaact gctttgtcca ctgtcctttg aagtatatgg
    12361 cgttgataag gtacattatt acatcatggg atataggcgg ttcaatcatt ttatcaattt
    12421 tcccttttgt tgccttgctg atccaaccat ttatagtatc agcagctttt gaatcctgaa
    12481 aatccatctc ggtaatatag gcattaaagt tatctttatt tacagacaaa aaatcctgtt
    12541 tgatctcctc accttttctg aaccaaattg agttgcttat attaagctgg acttttggat
    12601 ctacattatt aaggtacgcc aggaggtttt tatacccggt attcagattt tcaagctcta
    12661 tacccctata acgaagggct tcccccattg cttcttttgt ggttgacccg gcaccattgt
    12721 atgtcattgc aagcgcagtt gaaatactta gaggtgatat gaagatattc ttatcagcat
    12781 cttctgtatt caactctttg aatatgtcaa acgcgaataa ttcattccca tctgtaagac
    12841 tcgggtcaac tttatttaaa tcatatgatg cttgtttgct tccaccaata cgattgtttc
    12901 catttcccgc agaccccacg caccccataa aaagacaggc aactattata atagatatca
    12961 gcatacttat aattcttttc atccgtccca ctccttcata ttgatttttc agattaaata
    13021 ctttgtattt gcatcaaatg agtttgtatt tattttttga cggaatatgg atgcctgaag
    13081 ttccacaagc agcttgttat actttagggg aaacatggaa ctatatatta ttacttcttt
    13141 aaatcaagtc aaaggtccca catctgaaat atcaatctgc ttgaagccta gtgaaaaagc
    13201 cggagagtct ggttcaagaa caaagatgaa gttcataata tcagcaaatt tgggatctgc
    13261 acaaatcgag tggttatcat accccattct tttccattcg tccatatcaa tggtgttttc
    13321 aggtaacatg ccgtcttcaa gacttaagta ttcccacccc cctgcagcag ctatattacc
    13381 cttaacactc cagaaaaggt ttaaatcact tataaaattc tgctttttaa atttatcagt
    13441 tgacgaactt aaaaatacag gaggtccatc agtgacaaat atgttacgtt caattgtgaa
    13501 cgcaagatgg ttttccattg ttgacagagc tgcatttccc tccctgccaa aagcaaagat
    13561 gttgtttctc agtatgttct ctctcccata atgctggtgg aaacactggc ttcctgttct
    13621 gtaacagata ttattctcta taagtatatg agagctgcct tcatcagtat atattcccca
    13681 gcctccatag cagtattttt caatgtcgtg tacaacatta ccccatacta ccgtacccgg
    13741 ttggacacca agcatatata tgccacccat atcgctcagc aaaccatgcc ctatatcatg
    13801 tatataattt ttctcaatac ggttattttt tgatacactt tcactatagc cccataccca
    13861 gccacatgaa attccgctgt aataaagatc atgaatgtgg ttgtgggaaa tatcattccc
    13921 aaacgagttc atggatagta tcccacaact gctgtaaaat acctgacctg cagaataaat
    13981 atggttgctc gtaataacat tattcccggt caaccctgtt ccaggtcctt ttgcatctga
    14041 acctccgagt tttataccac ctgcacccat atcataaatt ttatttccag taattctgtt
    14101 gcccatgcag ccatcagcta tttcaatacc ataaaaccct gcatgcctta ttgtacagtt
    14161 ttttattgca cagcaacgtg cacccttcat agttatgaca ccgggaattg tatgtgcacc
    14221 ttgtacagat ggcccattat caaggttcca gtctccatgc tcaaatacca ggtcttcaaa
    14281 gcttaagaat tcaacatact tctcaacatc cggacatcct tcgattttca atagctgggt
    14341 agtacatgga gcaaatactt ccgctgtatc aggttcttca cctggaaatg gaatatagta
    14401 aagttttccc aatttacggt cgagatacca ttcaccaggt tcgctcaaag tctcaaatat
    14461 attttctata taatatctag gatatttttt ctcaaaatca tcacttagag ccattgtgct
    14521 cttccttgaa agtgtaacaa cccgggatac ttcatcatat gacttaatag gtatacgctc
    14581 ttcaatccat ttgtggaatg taactaattc aatgtcatta atgttttgcc actctttaaa
    14641 atcaccatct gcagctacaa aggcgtcctg cccatcccaa tatctatacc atggctctac
    14701 cgttaatccc gggacacttt ccatacggta aaacccatcc tttggaaatt tcgctctctg
    14761 acgtctttga tcatttacga aaagctgccg gaaataccac ttcccttctc ttacttcagg
    14821 tatatcagct atccagcagc atatagacga gtcagccaca gtaccaatac tccattctgt
    14881 tatacgcttg ctgccatgaa ttacaggctg ttcacccggg taagctttat agcaaacagg
    14941 tgctgaatcc tcaggtttaa aagtaactgg cttttcaact gcataccgtc caccccgtat
    15001 ccatacattt acaggaccgt caaacctgcc tgtgtacttt atttcaccaa tgatatcacg
    15061 ggctctctcg atagttgcaa caggtccatc tgtacctgtg ttgtctggat ttggcaaaag
    15121 acctgtccag ttatcatttc ccatcgtaga cacataaata tccatatatt ttcactcccc
    15181 ttgttctatt ttattcaact ttattcaaac tggggcaaat accgtatttg tgcctttatt
    15241 agctcatctg ccattttagc aacagcttta cgatctgata tgtaacctcc ggcgagaatc
    15301 gcttcttcca ttaacttcct gctgccttca agtgctgcgt caacagcaat ttcaattact
    15361 gataaaaatc ttgaagtaaa tcctgccagc acatcaggga aatcatccag atatataggt
    15421 tttggccctg ctgcagttgc caatacaggc atttcaataa tagcccatgg cggtagattt
    15481 ggtatagcac cattattttg tacattcata taataaacat ctctcgaatc tctctccata
    15541 gaatcaataa tacttgtaag ttgttcctgt tcaccgctta tatgtctgag gaaatcatca
    15601 tctaatgatt cggaagaaaa agcttttttc attgcttcat tgtggatttc atctcccaaa
    15661 gcaatagtat cttcaaagga gtatgcatca tatccaagag tataactata gtatttgcct
    15721 ccgggaaagt attccgtgaa aaattcggta atatgcctgt ctccaggtgc
//
LOCUS       DAOSDD010000093        15304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1550_length_15304_cov_5.202702, whole genome shotgun sequence.
ACCESSION   DAOSDD010000093 DAOSDD010000000
VERSION     DAOSDD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_15304_cov_5.202702"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..805
                     /locus_tag="PK733_09675"
     CDS             296..805
                     /locus_tag="PK733_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ10849.1"
                     /translation="MDELLVRKAIKGDRESFGQLIVLVRDHAYRVAYCYLRNEEDSMD
                     AVCDAVEKAFCNLKKLKEPRYFKTWFIRITINECKVQLRNRKKVEYLADDIFVQPDSN
                     FKDEKLDLNKVLGQLQAIDRLLIYMKYYMGYTLDEIAAATDMPAGTVKTRIYGNLKVL
                     KSRLEVSEA"
     gene            802..2274
                     /locus_tag="PK733_09680"
     CDS             802..2274
                     /locus_tag="PK733_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10850.1"
                     /translation="MKDVNEMYSKDISEICSDEKMLDQYMQDNMKDEIIVPGELEQKV
                     NARISALKRPIPRARYIIAAAALFILAFIGSIRFSPVIASYAAEIPGLAAVVDWLRGD
                     SGIRNAREHGYGGISPVVIEQDGFILEIANVFFDEDRLRFSAVVTGPEIAGMLNKTDS
                     MLPAVQDDSKSVQAGDELPLKDKNDNLHLQFDFKDFDNMGYIMTSHGQNSEYLAFDVE
                     KAFEPGEASAFIEKQTDMLNLGLKIKNGKSEVYAFDNIQLPFDASKFNFSDEYDQNKR
                     IVLQHTEIMIDSFIISPTRMKLDVSFNMEDGYFFTDFENPYLRDEKGNIYKGEGIISR
                     HSSMNERIMYFVPSTYFDRKPEKLYFCFDGIRIGSEEGKSFTLSLEEKYPKKLLYMGK
                     EISVTKVEWHEPGKLTVEFEYSTEDGIFRIQGVDIVDHKDIRSSGTGSYIWPYGASSN
                     FITKSLSEFEVEKCASYNMEFSNPGYLIPSDMDIELTLDK"
     gene            2567..3196
                     /locus_tag="PK733_09685"
     CDS             2567..3196
                     /locus_tag="PK733_09685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ10851.1"
                     /translation="MSNLIKDNSLVLLQGDSVTDCGRSRTKDTELGGGYANMIAALFS
                     AKYPEKNVRFINRGVSGNRVKDLQTRWKEDCLDLEPDWVSILIGINDCWRKYDSNDPT
                     SVEAYEKGYRDILKRTVNETDARLILCEPFVLPVPEDRKKWREDLDPKIQAVRLLAIE
                     FNAIYVPFDGIFAAASVKREPAFWAADGVHPTSAGHALMAIEWLKAVSA"
     gene            3211..4560
                     /locus_tag="PK733_09690"
     CDS             3211..4560
                     /locus_tag="PK733_09690"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HOJ10852.1"
                     /translation="MVPNSSYNICNYRDKKIQIKDIPENFNDLVKSLSIKNPPDIWLD
                     KWEESQKTYPGLDAIDFIKNDFIIDANKYLQLPDEAVQAILVTLNIIRNNEELARLLW
                     LSHYILFPSEIGVGIPDNLNNIKQHGVAFPDICIPQVNMDFLKGMFPAVLLLSGLLRL
                     LRFYKERSIPEQVIIDTLSDVDIWMEDYYAKNGTWGLMEFWWLYNHFSGRLFRIGRLQ
                     FIYAGFKGNVRVFRNIRTGVVVSLSEVDIRYRADGQVDGTNDIYDRHGAWVSTLVVTD
                     NSIKGNPISPLGFAENKVIELAKDEWREVLSKGSPVLDVHIATGSKLSHEACGESFKA
                     SVKFFERYFPELQFDGYMCTSWLLDPQLQCILPRESNIVKFQREFYLYPKKSDDFQTF
                     ERVFGNKPDNMKTLKAETSMQKAILDYVNKGNNMRQASMFLLKDDLQYWGQAFYQEQ"
     gene            4917..5243
                     /locus_tag="PK733_09695"
                     /pseudo
     CDS             4917..5243
                     /locus_tag="PK733_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
     gene            5496..7607
                     /locus_tag="PK733_09700"
     CDS             5496..7607
                     /locus_tag="PK733_09700"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOJ10853.1"
                     /translation="MQSESKVAEAPKLNAKASTTSETSNAGASASSDLQADNSSDYST
                     TNVQVQGVDEADIIKTDGKYIYQVNRDRIVIAKAYPAEEMKIESIISFNDAEFYPQEM
                     YLHGNKLVVIGNSYVRNIIPQNSDSGQTKNSGSLQTRDSALTEKKIYPQPYYYQSQTV
                     KAIMYDVSNKSDIKKLREFEIEGYYISSRKVGPYLYMVANKNIDYYYIMERSPVQIAP
                     VDPEISVKSNQDGFKSAPSYRDTASGDKFIDIDLKNVCYFPGIIQPNYMIVAGINLDK
                     DDEKANLSTYLGTGQNIYASLENLYVTVTNYSIIEQVQEINPQNNIEGSIKDAPTDGS
                     TTAVETNKDTASVAVTVTAKDVLADSNAAVTVTAKDAVTVIASAPAIKPNVAVIRRPP
                     MNQENTLIYKFSLNNGTVTYLGKGEVPGRILNQFSMDENDKYFRIATTRGEIWRNDEF
                     TSKNNIYVLDEMLNIKGKVEDIAPGEQIYSVRFMGDRAYMVTFKLTDPLFVIDLKNPQ
                     EPCILGALKIPGYSDYLHPYDENHIIGFGKDSVEIKGQAFYLGMKIALFDVTDVSNPI
                     QKFSETIGDRGTDSELLRNHKALLFSKEKELMAFPVTVMEIKSGSKFADGGFPNYGQF
                     TFQGAYVYNIDLIKGFTLKSRITHLDKDDMLKAGVSWYDNEKNINRIIYINDTLYTLS
                     NRMFKAHGLNDMKEKNIVTIP"
     gene            7986..8522
                     /gene="raiA"
                     /locus_tag="PK733_09705"
     CDS             7986..8522
                     /gene="raiA"
                     /locus_tag="PK733_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ10854.1"
                     /translation="MNFIVSGKNIDITEALRNTVVKKLGKLDKFFNPDTDVYVTMSVE
                     KNRHIVEVTIPFNGVVLRAEELSDDMYASIDEVIDVLEGQIRKNKTRLKRKLYNGAIK
                     PEYFAQIETQADVEEHEFKVVRTKKFAIKPMTVEEAILQMNLLGHQFFVFSNAESDEV
                     NVVYKRKDGNYGLIEPEF"
     gene            8707..9657
                     /locus_tag="PK733_09710"
     CDS             8707..9657
                     /locus_tag="PK733_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ10855.1"
                     /translation="MQYSKAKNINLSKFTLGTVQLGMNYGIANKSGKPDMNRSFNILE
                     TAIEEGINSFDTSLHYGDSERVLGKYFQTEKYEKRNCRSPLITTKFKLEADKNATPCE
                     IEKKIRERIESSLGNLGINKIPIYLAHDPKEMLMHKGVLADILKKLKNEGLIEKAGVS
                     VYNCEEADEMLKYDVYEAIQLPMNILDTRFVKSGILAKLHKAGIIVFIRSIFLQGLFF
                     LDPENLTGNLTQAGKYLNQLNELAQGEGMSVAQLALSYIRDMEEVTSLVIGAEAPQQV
                     KENVKLFEGPGITEKTRQNILDIFEVVPMHILVPSMWSVR"
     gene            9735..12125
                     /gene="pcrA"
                     /locus_tag="PK733_09715"
     CDS             9735..12125
                     /gene="pcrA"
                     /locus_tag="PK733_09715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOJ10856.1"
                     /translation="MNNDLLKKLNNEQREAVLYTEGPLLLLAGAGSGKTRVVTHRIAY
                     LIKEKGVLPGSILAITFTNKAAREMKERVEALIGEAGNDIWISTFHAACVRILRRDIE
                     KIGFDRSFVIFDTSDQQTVIKDCLKELNLNDKNYPPTNVLSEIGKAKDELIEPENFAR
                     KNESDFRMSKIAEIYKLYQQKLKQNNALDFDDIIMYTIKLFMDNPPVLEYYQKRFKYI
                     HVDEYQDTNTAQYTLISLLAHLHRNLCVVGDDDQSIYGWRGANIRNILDFEKDFKGAK
                     VIKLEQNYRSTKTILDAANNVIKNNMGRKKKTLWTDNDAGCKIKCFEGQNEHEEAFFA
                     ANEIKKIVQTENKDYKDFAILYRMNAQSRVLEEMLMREGIPYRIFGGLRFYDRKEIKD
                     VLAYLRLVQNLADNYAFKRIINVPKRGIGNVTVDAVESLAAKEECSMFDITCSAGEYP
                     DLQRSAAKLEKFRNLILELRALKDTLPVSVLIGEVIEKAGILGELENDKTTESESRIE
                     NIKELISAALDFEKRIDADQGEWQNEVEDKSLEAFLAHISLVADIDNLNEERNNVVLM
                     TLHSAKGLEFPVVFMVGVEEGIFPSYRSLYSESELEEERRLCYVGITRAMDKLYITNT
                     HCRTLFGKTTNNRPSRFVDEIPHRLIDGHFPLNQNDEYDEDDDEFDIDCSEDRDYKGY
                     SYKDNNYRYNSYKDNSYRDNNYYSDYYNNKGHYKKDHENSFTRNSSVGKANSKPGTSS
                     RVKFNIGDFVMHKKFGKGVITNIEKEKEDYKLEIIFNNVGMKRLMAAYANLEKIDK"
     gene            complement(12244..12894)
                     /locus_tag="PK733_09720"
     CDS             complement(12244..12894)
                     /locus_tag="PK733_09720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ10857.1"
                     /translation="MKYKAVIFDLDGTLLDSIDDLADSMNAVLEDAGYPAHDIQSYKY
                     FVGDGMEKLVFRSLPEEYRDTETVKKYAGKMKQEYEKRWNNKTRPYQGIPELLDALSS
                     RDVHIAVLSNKPDNFTKLMIKELLPNWEFRAVYGDRNYIPRKPDPAGAIEIARTMGLQ
                     SREILYLGDTGTDMQTACSAGMYAVGALWGFRGKDELLANGAKALIEKPLDLLNLL"
     gene            13374..14435
                     /locus_tag="PK733_09725"
     CDS             13374..14435
                     /locus_tag="PK733_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10858.1"
                     /translation="MEIIINNGVTPYGRVITTLRGGCPDKIPFTVYENMLPRCAAERD
                     LRNRGLCIVKRITSYNIRRPNVKTKSVHYLDEKGRDLIRTIYETPYGELSTLDQPAGF
                     TSWHHERLFKSPDDYKALLFMIKDSIIEPAYDNASKVVKDLGEDFIVRDNLPLEPMQN
                     LVSSYMGAETFSYEWMDNRDEVLKLYDAFVDVARKIYPIVAGGPLEFSNYGGNVIPQV
                     IGVENFKKYYVPHYNEAAEILHKKGKLIGCHLDADNKIIMNAVADTDLDYIEAYDPGI
                     SPPVKEARKIFGSKVLWINWPSAWHLNSVNNVLKDTIKLIEEAKPGNGFIIGITEDVP
                     EERWKENFLAIMDGIDSCK"
     gene            14613..15032
                     /locus_tag="PK733_09730"
     CDS             14613..15032
                     /locus_tag="PK733_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOJ10859.1"
                     /translation="MEYRVFGDKIVARIDKGEEIIESIKKICIKEKTALGKVSALGAV
                     NRAKIGLFETGIKKYHSAELSGDFEITSLSGTITEMDGEPYLHIHINLAGADHKTFGG
                     HLNEAYVSATCELVIDVINGRVGRKFSKEIGLNLMEF"
     gene            15082..15303
                     /locus_tag="PK733_09735"
     CDS             15082..15303
                     /locus_tag="PK733_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HOJ10860.1"
                     /translation="MDPEYFAIFEVKYIKKSEYEMYGEKIVEEKKKEATVQIMKYHTS
                     GELQKLPQLKKWALVFAGDKCVVNNEIIL"
BASE COUNT         5316 a         2297 c         3191 g         4500 t
ORIGIN      
        1 agcgtaacgt cagctcctgg aaaatatttc ttcactttta aaatatgcac ccctgttaat
       61 tgcttctctt ttacgtttca tatatatccg ctccatttca atatttattt tatttattac
      121 atcatgctgc gattatcata gactttacat acaaaatgtc tgctcgtaat attagactac
      181 tgttattaaa agatattaca tctgactcca gatttgctat aattcttaat aaatatataa
      241 attttaaaac tttttgcctc ttatatgcat ctaatgtttg aaaggaggtt gaagtatgga
      301 tgaattgttg gttagaaagg ccattaaggg cgaccgtgaa agcttcggac aattaatagt
      361 tttggtaagg gatcatgcat accgggttgc ctattgttat ctcagaaacg aagaagatag
      421 catggacgct gtatgtgatg ctgtagagaa ggctttctgt aatttaaaaa aattaaagga
      481 acccaggtac ttcaaaacat ggtttattcg aatcactata aacgaatgca aagtgcagtt
      541 gaggaacaga aagaaggttg aatatctggc ggatgatatt tttgtgcagc ctgattccaa
      601 ctttaaggat gaaaaactgg atctcaacaa ggttttagga cagcttcaag caatagatcg
      661 gttgctgatc tatatgaaat attatatggg atatacactc gatgaaatag cagcagccac
      721 agatatgcca gcaggcactg ttaaaacaag gatttatgga aatcttaagg ttcttaaaag
      781 cagattggag gtaagtgaag catgaaagat gttaatgaaa tgtattctaa agacatcagc
      841 gaaatatgtt ctgatgagaa aatgctagat caatatatgc aagataatat gaaggacgaa
      901 attattgtac cgggggagct ggaacaaaag gttaacgcaa ggatctccgc acttaagcga
      961 ccaattccga gagcaagata cattatagca gcagcggctt tatttatact tgcatttata
     1021 ggaagcatac gtttcagccc ggttattgct tcgtacgcag cggaaattcc gggtcttgca
     1081 gctgtagtag actggctgag aggagacagc ggtataagga atgcccgtga acatggttat
     1141 ggaggtataa gtccggtagt aattgaacag gatggattca tactggaaat agctaatgta
     1201 ttttttgatg aagaccgttt aagattttca gcagttgtga ccggtcctga aatagccggt
     1261 atgctgaaca aaacagacag tatgctgcct gcagtacaag atgacagtaa atcggttcaa
     1321 gcaggtgatg aacttcccct aaaagataaa aatgataatc tgcatttaca atttgatttt
     1381 aaggattttg ataatatggg atatattatg acctctcatg gacagaacag tgaatatctt
     1441 gcatttgacg ttgaaaaagc ttttgaaccc ggtgaggcgt cagcttttat agaaaaacaa
     1501 acagatatgc ttaatttagg gcttaagatt aaaaatggca aaagcgaagt ttatgccttt
     1561 gataatatac agcttccgtt cgatgcttca aagtttaatt tttcagatga gtacgaccaa
     1621 aacaagcgta ttgttctgca acatacagag attatgattg acagctttat aataagtccg
     1681 acaagaatga agcttgacgt aagctttaac atggaagacg gttatttttt taccgacttt
     1741 gagaatccct atttgaggga tgaaaagggc aatatttata aaggagaggg aataatatcg
     1801 aggcatagca gtatgaatga aagaattatg tatttcgttc cctcaactta ttttgacagg
     1861 aagcctgaaa agctttactt ctgctttgac ggtatcagaa tagggagtga agagggcaaa
     1921 agctttacat taagtctgga ggaaaagtat cctaaaaagc ttctgtacat gggtaaggaa
     1981 atcagtgtaa caaaggtgga atggcatgag cctgggaaac tgacagtaga gtttgagtac
     2041 tcaacggagg atggaatctt cagaatacag ggagttgata ttgttgatca taaagacata
     2101 aggtcaagtg gcacaggaag ctatatatgg ccatatggtg caagttccaa tttcattacg
     2161 aaaagtttaa gtgaatttga agttgaaaag tgtgcctcct acaatatgga gttttctaac
     2221 cccggatatc tgataccatc agatatggat attgagctta cactcgacaa atagtatgtg
     2281 tcagtaatta ataggcgcat tcaagaaatt gcgcctgctc cccccttttg gtaagtattg
     2341 catttcattg atttttatta tatattatct ttgatataat taatacatgg agatatatta
     2401 atatatgtat atattgatcc agctgcagga agtcatgttc caacaaaatt tcgatagcat
     2461 tggaagatgt tacgagggaa aatgtctcta aagcggacat tgcaatactt tcctcaggaa
     2521 tatttccagc ttatattcag gtataatata tgaagggatg gtgcttatgt caaatttaat
     2581 aaaagataat tctcttgtac ttttacaggg agatagtgta acagattgcg gccgcagccg
     2641 tacaaaggat actgaacttg gaggcggata tgcaaatatg attgcagctt tattttcagc
     2701 taaatatccc gaaaaaaatg taaggtttat aaacagaggg gtaagcggca atagggtgaa
     2761 agaccttcaa acaagatgga aggaagattg tttggattta gagccggact gggtctccat
     2821 acttattggt attaatgatt gctggagaaa atatgacagt aacgacccaa cttctgtaga
     2881 agcgtatgaa aaaggatata gggatatatt gaaaagaacg gttaatgaaa cagatgcacg
     2941 ccttattcta tgtgagcctt ttgttttacc cgtaccagaa gatagaaaga aatggagaga
     3001 ggatctagat ccaaaaattc aagctgtaag gctgcttgca atagaattta acgcaatata
     3061 tgttccattt gatggtatat ttgcagcagc atctgtaaaa agagagcctg cattttgggc
     3121 agctgatgga gtacatccaa ccagtgcagg gcatgctctt atggctattg aatggctcaa
     3181 ggctgtgagt gcttaaagag gtgattttgt atggttccta atagtagtta taatatatgt
     3241 aattatcgtg ataaaaaaat acagattaag gatatacctg aaaattttaa tgatttggtt
     3301 aaatctctca gcattaaaaa tccgccggat atatggcttg ataaatggga agaatctcaa
     3361 aagacatatc ccggactgga tgcaatagat tttattaaaa atgattttat cattgatgcg
     3421 aacaaatacc tgcaacttcc ggatgaagcg gttcaggcca tcttggtaac acttaatata
     3481 ataaggaata atgaagagct tgcaagatta ctatggttaa gtcattacat acttttccca
     3541 tctgagatag gggttgggat tcccgataat cttaataata ttaagcagca tggagtagca
     3601 tttccggata tttgcatacc tcaagttaat atggactttt taaaaggaat gtttcctgca
     3661 gttttgcttt tatccggtct gctgcgtttg ttgagatttt ataaagagag aagtatcccg
     3721 gagcaggtga taattgatac cttaagtgat gttgatatat ggatggagga ttattacgct
     3781 aaaaatggaa cttggggatt aatggagttc tggtggttgt acaatcattt ttcaggcaga
     3841 ttattcagga ttgggcgact gcaatttatt tatgcagggt tcaaagggaa tgtaagagtt
     3901 tttcgcaata taagaacagg agtggttgtt tcactatccg aagtggatat caggtacaga
     3961 gctgatggtc aggttgatgg aactaatgat atttatgacc ggcatggagc atgggtgtca
     4021 acacttgttg ttactgacaa ttcaataaaa ggcaatccta tttctcctct gggttttgca
     4081 gaaaacaaag taatagaact ggctaaggat gagtggagag aagtgctttc taaaggtagt
     4141 cctgttcttg atgtgcatat tgctaccgga agtaagcttt cccatgaagc atgcggcgag
     4201 tccttcaaag catctgttaa attttttgag agatactttc ctgaactgca atttgatgga
     4261 tacatgtgca cgtcctggct tcttgacccg cagcttcagt gtattttacc ccgggaatca
     4321 aatattgtaa agtttcagag ggagttttat ctatatccta aaaagtcaga tgattttcaa
     4381 acctttgaaa gggttttcgg aaataaaccg gataatatga aaacacttaa ggctgaaacc
     4441 tcaatgcaaa aagccatact tgactatgtt aataaaggaa acaacatgcg tcaggcatcc
     4501 atgtttctct taaaggacga tttgcaatat tgggggcaag cattttacca ggaacagtaa
     4561 atgtaagttt caatttattt ttttagagtt ttcattaatc aatacactta tactttctat
     4621 caacatactt ttcttaagcg tagagggctt gattgagagc cctcattttt ttgtatgatg
     4681 gaacttttta taatttcact tacgtcaaat atatgaaatc agttaaggaa aataatacat
     4741 aaaaagatat tagaattgaa aggaggatat ttatcatgaa aaaatcaatc agacttccga
     4801 ttatattaat tattgtcatg attactgttg tcatatttgt gatggcacca ttcagcaaga
     4861 ttaatgactt taattctgtg gttcatgctg aagacaaatt gaaaaattca gttataatgt
     4921 acattggaag tcctaatgca attgtaaatg gctccagggt tttagttgac aaattaaacc
     4981 ctgaacttgg acctgttata tcgaatgata cagttcttat tcctttgaga tttactgctg
     5041 aaggtttcgg agcagaagta ggatgggacg gaaaaactgc agctattacc gttgatctgg
     5101 gcaaaagcaa cattaaatta aaacagggcg gtgacacaat aatttctaat ggtcgtgagt
     5161 ataaattaag ctctcctgtt acagctataa aaggcagaac ttatgtaccc ctggagattt
     5221 ttgctgaagc cttgggtaaa aaagttttca acaaccgggg tctcatagtt attagtgata
     5281 atgaaaatat attagattcg gaggaagaca aatctttaat agacgatctt gtttccagga
     5341 ttggagggct tccgacagtt ggttcttatg aaaagctggt tgaattatta atgcctttaa
     5401 tggcagctaa ttcatcagaa ataatgtatg gaacaagacc cgcaatagct ccttcaatag
     5461 ctatggatgt taaaagagat gatgattcta taactatgca atcagaatca aaagtggcgg
     5521 aagctccgaa attaaatgct aaagcatcca ctacttcaga aacatcaaat gcgggtgctt
     5581 ctgcttcatc tgatttacaa gcagacaact catcggatta ttctactaca aatgttcagg
     5641 ttcagggcgt agatgaagcg gatattatta aaacggacgg aaaatatata tatcaggtga
     5701 acagggatag aatagttata gcaaaggcat atcctgcaga ggaaatgaaa attgagagta
     5761 taattagttt caatgacgca gaattttacc ctcaggaaat gtatctccat gggaacaagt
     5821 tggtggttat aggtaattcc tatgtaagaa atataatacc tcagaactca gatagcgggc
     5881 aaaccaagaa ctccggcagc ttgcaaaccc gggacagtgc gctaactgaa aagaaaatat
     5941 atccacagcc ttattactat cagagccaaa ctgttaaagc tattatgtat gatgtaagca
     6001 ataagtctga tattaagaag ctcagagaat ttgaaattga aggctattat atatcttcaa
     6061 gaaaagtagg accttacctc tatatggtgg caaataaaaa tatagattac tattatatta
     6121 tggaaagaag cccggttcag attgccccgg ttgatcctga aatatctgtt aaaagcaatc
     6181 aggatggttt caaatctgca ccttcttata gagatactgc atcaggagat aaattcattg
     6241 acattgatct taaaaatgtt tgctatttcc cgggtattat tcaaccaaat tatatgattg
     6301 tggcaggcat taatcttgat aaggatgatg aaaaagctaa tttatcaaca tacttgggta
     6361 caggacaaaa tatatatgct tctcttgaga atttatatgt tactgttaca aattatagca
     6421 tcatagaaca agttcaggaa ataaacccac aaaataatat agaaggtagc attaaagatg
     6481 caccgacaga tggtagcacc acagcagtgg aaacaaataa agatacagca tcagtcgctg
     6541 tcacagtaac agctaaggat gtgttggcag atagtaatgc tgcagtcaca gtcacagcta
     6601 aagatgcagt aacagttatt gcatcagctc cagctattaa acctaatgtt gcagtcatta
     6661 gaagacctcc catgaaccag gaaaatacat taatatataa attttcattg aataatggta
     6721 ctgtcaccta tcttggaaaa ggggaagtac ctggccgtat tcttaatcaa ttttcaatgg
     6781 atgagaatga taaatatttc cgcattgcaa caacccgggg agaaatatgg cgtaatgatg
     6841 agtttacatc taaaaataac atttatgtac tggatgaaat gcttaatata aaaggtaaag
     6901 ttgaagatat tgctcccggt gagcagatat attctgtaag atttatgggt gacagggcat
     6961 atatggttac tttcaaactt acagacccat tgtttgttat agacctaaag aacccgcagg
     7021 aaccatgtat acttggagca cttaagatcc cgggatatag tgattatctg cacccttatg
     7081 atgaaaacca tataatcggg tttggaaagg attctgttga gataaaaggg caggcttttt
     7141 accttggtat gaaaattgct ttgtttgatg taacggacgt ttccaatccc atacaaaagt
     7201 tctcagaaac aataggagac aggggaactg actcggaact ccttagaaat cataaagcct
     7261 tgcttttctc aaaagaaaaa gagttgatgg catttcctgt tacagtcatg gaaataaagt
     7321 caggtagcaa atttgcagat ggtggattcc caaattatgg acagtttaca tttcaaggcg
     7381 catatgtgta taatattgac ctgattaaag ggtttacttt aaagagcagg ataactcatc
     7441 tagataagga tgacatgctt aaagcaggag taagctggta tgacaatgag aaaaatatta
     7501 acagaattat ttacattaat gacacattat acacattatc aaacagaatg tttaaagctc
     7561 atggattaaa tgatatgaaa gaaaagaata tagtgacaat tccttaaaaa aagcagtaat
     7621 tgtttacata aaacaagttc ctgttaatat aaataacaac agagttatcc acaggagaaa
     7681 tagtgataaa taagttattc acagctttat acacattatc cacaggattt ttatatacaa
     7741 aacgcatgtt ttgtgggaaa actgtggaaa tgtacttcaa atatatgaaa tttaaataaa
     7801 aactcatagt cgcagtttta gaattatcac aattaattat gtataaattg tggataaatt
     7861 ttagaggaat tacaagtttt atgtcgaatt atattatatg attgaaattt ataaaaaagt
     7921 tgtttggtat attaatttgt tggtaagaat attaatatta ggtaaaagcg aaaggggttg
     7981 tagttatgaa ctttatagta agcggtaaga atattgacat tacagaagca ttaagaaaca
     8041 ctgttgttaa gaaactgggc aagctggata agttctttaa tcctgatact gatgtttatg
     8101 ttacaatgag tgttgaaaaa aacaggcaca tagttgaagt tacaattcct tttaatgggg
     8161 tagtgttaag agccgaagaa ttgagtgacg atatgtatgc ttctatcgat gaggttattg
     8221 atgtccttga aggccagatt aggaaaaaca aaacaaggct gaagaggaag ctctataatg
     8281 gggcaattaa gccggagtac tttgctcaga ttgaaacaca agctgatgta gaagagcatg
     8341 agtttaaagt agtaagaacc aagaaatttg ctataaaacc catgactgta gaagaagcta
     8401 ttctgcaaat gaatttactg gggcaccaat tctttgtatt ctccaatgca gaatctgatg
     8461 aagtaaatgt tgtttataag agaaaagatg ggaattatgg acttattgag ccggagtttt
     8521 agcagacaga tattataaac aatatataaa gtgaatagaa tcaagtgagt agatatcaag
     8581 tgaagtagat ataaagctgg tagatatgag gatgatagat attattcgcc ataagtatta
     8641 agcggggcag cttatttgac cccgctttaa attttttatg atttttgtaa aggagcgtat
     8701 aaaataatgc aatattccaa agcaaaaaac attaatctat cgaagtttac tctcggaaca
     8761 gtacagttgg gaatgaatta cggaatagcc aacaaaagtg gcaagcctga catgaacaga
     8821 agttttaata ttttggaaac tgcaattgaa gaaggaatta attcatttga tacttcattg
     8881 cactatgggg attctgaaag ggtacttggt aaatattttc aaacagaaaa atatgagaaa
     8941 cgtaattgca ggtccccact aataacaact aaatttaagc ttgaagcgga caaaaatgct
     9001 acaccctgtg aaattgaaaa gaaaatccgt gaaagaattg aatcatctct tggaaatctt
     9061 ggcattaata aaattcctat ctatcttgca catgatccca aagaaatgct aatgcataaa
     9121 ggtgttctag ctgatatcct taagaaactt aaaaatgagg gattaattga aaaggccggt
     9181 gtttctgtat acaattgtga agaagcagat gaaatgctta aatatgatgt atatgaagca
     9241 attcaacttc ccatgaatat tcttgatacc agatttgtaa aaagcggcat tctcgctaaa
     9301 cttcataaag ccggaataat tgtatttata aggagcatat ttttgcaggg actgtttttt
     9361 ttggaccctg aaaacctgac aggtaatctg acccaagcag gaaaatacct taatcagctg
     9421 aatgagctgg cgcaaggaga gggtatgagt gtggcacagc ttgctctttc ttatataaga
     9481 gatatggaag aggtaacaag ccttgtaata ggtgctgagg caccacaaca ggtcaaggaa
     9541 aatgttaaat tatttgaagg acccggtatc accgaaaaaa ccaggcagaa tatactggac
     9601 atctttgaag ttgtaccaat gcatatttta gtaccaagta tgtggagcgt gaggtaaaaa
     9661 attacaggtt gctctattcc tgagaatcta atataataga taaatactat tattgaactg
     9721 gggaagataa aaggatgaat aatgatttac ttaaaaagct gaacaatgaa cagagagaag
     9781 ctgttttata tacagaaggt ccattacttt tactcgcagg tgcaggaagt ggcaaaacca
     9841 gggttgttac ccatagaatt gcctatctaa ttaaagaaaa gggagtactt ccaggaagta
     9901 tactggctat tacttttaca aataaggccg ccagagagat gaaggaaaga gtggaagctc
     9961 tcataggcga agctgggaac gatatatgga taagcacttt ccatgcggct tgtgtgagaa
    10021 tcttaagaag agatatagaa aaaatcggct ttgaccggag ttttgtaata tttgatacct
    10081 cagaccagca gactgtaatc aaagactgct tgaaggaact taatcttaat gataaaaact
    10141 atccgcctac aaacgtactg agcgaaatag ggaaagctaa agatgagtta atagaaccgg
    10201 aaaacttcgc caggaagaat gaaagtgatt tccggatgag taaaattgcg gaaatataca
    10261 aactttatca gcaaaaatta aagcagaata acgcattgga ttttgatgat ataattatgt
    10321 ataccattaa gctctttatg gataatcctc cagtgcttga atattaccag aaaaggttta
    10381 aatatataca tgtagatgaa tatcaggata caaatactgc tcagtatacc ttgataagcc
    10441 ttcttgccca tttgcacagg aatctttgtg tggtcggcga tgatgatcag tccatatatg
    10501 gatggcgcgg ggcgaatata agaaatatcc tggactttga aaaagatttt aaaggtgcaa
    10561 aagtaattaa gctggaacaa aattatcgtt ccactaaaac cattctggat gctgcaaaca
    10621 atgttattaa aaataatatg ggaagaaaaa agaaaactct gtggacagat aatgatgcag
    10681 gctgcaaaat aaaatgtttt gaaggccaga atgagcacga agaagccttt ttcgcagcaa
    10741 atgagattaa aaaaatcgtc cagactgaga acaaagatta caaggatttt gcgatactat
    10801 acagaatgaa tgcccaatct cgtgttcttg aggaaatgct tatgagagaa ggcatcccct
    10861 accgtatttt tgggggtttg aggttttatg atagaaaaga aattaaggat gtacttgctt
    10921 atttaagact tgtacagaat ctggcagata attatgcttt taaaaggatt attaatgtac
    10981 ccaagagagg tatcggaaat gttacagttg atgcggttga gagcctagcg gccaaggaag
    11041 aatgcagcat gtttgatatt acatgctcag ccggtgaata tccggatctc cagaggtcgg
    11101 cagctaagct ggagaagttt agaaatttga tacttgaatt aagagcattg aaagacactt
    11161 tgcctgtatc tgtattaata ggtgaagtaa ttgaaaaagc aggaatcctg ggcgaattgg
    11221 aaaatgataa aacgaccgag tctgaatcca gaattgaaaa tattaaggaa ctgatttccg
    11281 cagcactcga ttttgaaaaa cggattgatg cggatcaagg tgaatggcag aatgaagttg
    11341 aagataaaag cctggaagca tttttggcac atatttcact tgtagcagat atagataacc
    11401 ttaatgaaga aagaaataat gtagtgctaa tgactttgca cagtgccaag ggattggaat
    11461 ttcccgtggt gtttatggtc ggagttgaag agggcatatt ccccagttac cgttctcttt
    11521 atagcgagag tgaactggaa gaggaacgca ggctttgcta tgtaggaatt accagggcaa
    11581 tggataaact ttatataacc aatacacatt gcaggacatt atttggtaag actaccaata
    11641 ataggccatc gaggtttgtg gatgaaattc cgcacaggct catagatggg cattttcctt
    11701 taaatcaaaa tgatgaatat gatgaagatg atgatgagtt tgatatagat tgttccgaag
    11761 atagggatta taaaggctat agttacaaag ataataacta tagatataat agttataaag
    11821 ataatagcta tagagataat aattattata gcgattatta taataataaa gggcactata
    11881 aaaaagacca tgaaaacagt tttactagaa acagctctgt tggcaaggca aatagcaaac
    11941 caggtacaag cagcagggta aagtttaata ttggcgattt tgttatgcat aaaaaattcg
    12001 gaaaaggcgt tataactaat atagaaaaag aaaaggaaga ttacaagctg gaaataattt
    12061 ttaataatgt ggggatgaag aggcttatgg cagcttatgc aaatcttgag aaaattgata
    12121 aatgaatcca catatagact gaaaatgagg ggtgcatatt ttaaaagtga agaaatattt
    12181 accagtattt tctttactta aattatatgc acccaaaagc gagtcaacat attgactgct
    12241 gactcacaat aaattcaaaa ggtctagggg cttttctatc agagcttttg ctccattagc
    12301 cagtagctca tcctttcctc tgaaccccca taatgcacca accgcataca tacctgcact
    12361 acatgcagtc tgcatatctg tacctgtatc accaagataa agaatttctc tggattgcaa
    12421 tcccatagtg cgggcaattt ctatagctcc tgcgggatca ggtttcctgg gtatgtaatt
    12481 ccgatcacca taaactgctc taaactccca attagggagc aattccttta tcataagttt
    12541 agtgaagtta tcaggcttgt tggataatac cgctatatgt acatcccttg aagaaagtgc
    12601 atcaagcaac tcaggaatac cctgataggg acgggtctta ttattccacc ttttttcata
    12661 ttcctgcttc attttaccgg cgtatttctt taccgtttct gtatctctgt attcttccgg
    12721 gagggatcta aataccagct tttccatgcc atcccctacg aaatatttgt agctttgaat
    12781 atcatgagcc gggtatcccg catcttccag cactgcattc attgaatcag ccagatcatc
    12841 aatagaatcc aataatgtac cgtccaaatc aaaaatgact gctttatatt ttatcatttg
    12901 taaccacctt cctttatggt ttgtatagtt tgtgtatttc cccaataaat tagaaaatta
    12961 cattgcgaaa tcaaagttaa tttttgcagt tgatctacta ttaccttata ttataccaca
    13021 acttggcatt gtttagttaa aatatatgga acatgttatg ctccttacca atattttctt
    13081 cagataaatc aaatgcaccc aataaaatta aataaattgt gccagtcaag gaaatagggt
    13141 ataattgtta atataacgag gcaataggtg tccttcacct ctgctgatgt gttataaaag
    13201 gctctaaaga ttctaactca aacaatctac aaactgactt taaactggct ttgtatgaaa
    13261 attgatttcc agggcttaaa aagagaggat ttgggatgaa aagacttcca tatagtagga
    13321 aatatagaag caatatttta ccgcattaac taaaaaaggt tggtgaaaac aatatggaaa
    13381 taattataaa caatggcgtg acaccgtatg gcagagtgat aacaacatta aggggagggt
    13441 gccctgataa aatacctttt acagtatatg aaaatatgtt accccggtgt gctgctgaaa
    13501 gagaccttag aaacagaggc ctttgcatag ttaaacgcat aacctcttac aatattagac
    13561 gacccaatgt aaaaacgaaa tccgttcatt atttggatga aaaaggcagg gatttgatac
    13621 gcacaattta tgaaacacca tatggtgagc tttctactct tgatcaacct gcgggtttta
    13681 catcatggca tcatgaaagg ttatttaaat ccccggatga ctataaagct cttctattta
    13741 tgattaaaga cagtataatc gagcctgcat atgataatgc ttcaaaagtt gtcaaagacc
    13801 tgggcgaaga ttttattgta cgtgacaatc tacctttgga gcctatgcaa aatcttgtat
    13861 catcatacat gggagcagaa acattttcat atgaatggat ggataacagg gatgaggttt
    13921 tgaaactcta tgatgcattt gttgatgttg caaggaaaat ttatcccatt gttgctggtg
    13981 gaccattgga attttcaaat tatggaggga atgtaatacc tcaggtaata ggagtggaaa
    14041 attttaaaaa gtattatgta ccacactata atgaagctgc tgagatattg cataagaaag
    14101 gcaaacttat agggtgccat ctggatgcag ataataaaat tattatgaat gcagttgcgg
    14161 atacggatct tgattatatc gaagcatatg accccggcat aagccctccg gttaaagagg
    14221 cacgtaaaat ttttggcagc aaagtattat ggatcaactg gccgagtgca tggcacttga
    14281 attcagttaa taatgtttta aaggatacta ttaaattaat tgaggaagca aagccgggta
    14341 atggatttat tatcggcata acagaggatg tgcctgagga aagatggaaa gaaaattttc
    14401 tggcaattat ggatggtatc gacagttgca aatgattgca tgtatagtat attatgcata
    14461 aaattggtaa ttacaaataa ctatatgtgt gatatattat gtaaaaattt ttggatgaat
    14521 gtaattataa gcagttatta aagtgaataa ttactaattg aataattaaa tgatgaacat
    14581 aaatatatta cataggtatt tggaggtgtt ttatggaata cagagttttt ggtgataaaa
    14641 tagttgcccg aattgataag ggagaagaga ttattgaatc tataaaaaag atctgcatta
    14701 aggaaaaaac agcacttggt aaagtatctg ctttaggtgc ggtaaacagg gctaaaatag
    14761 ggctgtttga aacaggcata aaaaaatacc attctgcaga actatcggga gattttgaaa
    14821 taacttcatt atcaggtaca ataaccgaaa tggatggaga gccttatctt catatacaca
    14881 ttaaccttgc aggcgcagat cacaaaacat ttgggggaca cctgaacgag gcatatgtaa
    14941 gtgcaacctg tgagcttgtt attgatgtca ttaatggacg ggtgggaaga aagttcagta
    15001 aggaaatcgg acttaactta atggagtttt aaactggctt tgtatgaaaa ttgattttta
    15061 gggcttaaaa ggagagaacc aatagatcct gagtatttcg caatatttga agttaagtat
    15121 attaaaaaat cagagtatga aatgtatggc gaaaaaattg tggaagaaaa gaaaaaggaa
    15181 gctacagtac aaattatgaa gtatcataca tctggcgagc ttcaaaaatt accacaactt
    15241 aaaaagtggg cgcttgtatt tgcaggggat aaatgtgttg tcaataatga aattatacta
    15301 tagg
//
LOCUS       DAOSDD010000094        15294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1553_length_15294_cov_5.349498, whole genome shotgun sequence.
ACCESSION   DAOSDD010000094 DAOSDD010000000
VERSION     DAOSDD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1553_length_15294_cov_5.349498"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK733_09740"
     CDS             <1..335
                     /locus_tag="PK733_09740"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HOJ10861.1"
                     /translation="EIYPVKKNLLKETTKKRMANTFQLFKKAAGEQTTIPYHGQEIDE
                     DYILIQNIKDAKNEWLNADLNLQYVNETEFVEYYIYKLKAAQIKYEFFLKKAKERGLK
                     IRIDTGIQ"
     gene            378..620
                     /locus_tag="PK733_09745"
     CDS             378..620
                     /locus_tag="PK733_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOJ10862.1"
                     /translation="MLNKHIFLFILRRLAMAPLKIIFKLVGKVIIGGIVLVLINMVGR
                     LFGFHISINVISAFVTGTLGVPGVILIIALKSIFRF"
     gene            890..1879
                     /locus_tag="PK733_09750"
     CDS             890..1879
                     /locus_tag="PK733_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HOJ10863.1"
                     /translation="MYDVIDSIIVSKYGLKIKSSIPFREGFILATSKGRFLCKETRMK
                     AGRILFVHSAKEHLVNNGFKNLDRYLCTQEGEPYVINNGKCYIVSYIIEGKECDFENR
                     NDVKRASNLLACMHAASKGYIPPAGSLIRDDVGKTPSLFCKRLKEIKRMEKIASRRKS
                     NFDYIYAAYSNYFYEVGKDALDYILSPIYDKLVNETRLEKIFCHNDLTYSNLILNDNR
                     DSLINFDFCCYDLKVYDLANLIRRRMRRCNWDIREAKVITDEYNSVNKLTPEDFYVMK
                     IMLQFPQKFWRVANKYYNSRRSWAERALTDRLEDVINEIDCHKKFLDNYEIII"
     gene            1960..2856
                     /gene="yabG"
                     /locus_tag="PK733_09755"
     CDS             1960..2856
                     /gene="yabG"
                     /locus_tag="PK733_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOJ10864.1"
                     /translation="MGNVKVGDIVARNSYGCDIFFKVVEIKSDGKQNIITLKGITYRI
                     QADAPESDLIVQSDANVREYRNKVDTEAYRKLKQTEYYSGRMGNRVAWPKKGFHRDTS
                     NDDTRTFTRPGKILHFDGDEDYLETCIKEYIKRGVDAVGKHIAEKEQPSRIYKLLQEY
                     KPDILVITGHDSISKDQKDYLNINSYVNSRYFIESVKEARRYNPDMDSLVIFAGACQS
                     LYDGIIKAGANFASSPDRVLIHALDPVLVAEKLSLAPIDRVVMPMDVINNTITGATGI
                     GGLQTRGKFRKGYPKEPYTGNI"
     gene            3248..3514
                     /locus_tag="PK733_09760"
     CDS             3248..3514
                     /locus_tag="PK733_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOJ10865.1"
                     /translation="MAEKSDLLEIKRNIETCIGEKVQLKSNKGRKRTFIREGVIENTY
                     PSIFTIKFENEYDMTRRVSYSYTDVLTNAVELIVCRNNRRIQVS"
     gene            3719..5293
                     /locus_tag="PK733_09765"
     CDS             3719..5293
                     /locus_tag="PK733_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOJ10866.1"
                     /translation="MSLDLAREVIKVSNIINREITQIIVENDIIVPDTKPDVVKILIA
                     EAEAYTTSAVVNTDEKSTFIKGIIRYKILYKPDDPDIDIKSININAEFSHEMDTPVAG
                     KDVRNIVKCLAEHVECEIINGRKINTKTILQLNSKVMAEEEYVLINDISDNGDIQVLR
                     NNMEINNFIGSSEALYTIKESMEVPEGNPPIREILSNDVKIINKDFRLSDNKVIVTGE
                     MNIVTLYAGDDQESSIRLMEHATPFTQFVDLSGINDESDCEIGYEVRDYSFEAAEDAD
                     GELRVMNMEVEVSINVYGSVRKNIDIVHDAYGLRSGVTLDKKVVKTEEKVTENISQAV
                     LKDTATIGEGNPEIVEIINMVSKPVLTDYKVQEDKIEIEGFVANNLIYLSNNSEQNMS
                     CLQSDIPLKQTIDIKGVKPGMACEIELLTEHANYSMLSSKDVELRLVVGVVSKVYKNV
                     EKHIIEKAIEIPFDESRKADKKAGIIVYFIQPGDTLWEIAKKYTTTVNDIQRVNMFQD
                     QDSIDDGMQILITRKS"
     gene            5552..6541
                     /gene="ispE"
                     /locus_tag="PK733_09770"
     CDS             5552..6541
                     /gene="ispE"
                     /locus_tag="PK733_09770"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOJ10867.1"
                     /translation="MQLNMQQNIMDFCPYRTISLMARAKVNLALDVTAKRLDGYHEIK
                     TIMQTIKLHDKVIIDVIDKGIEIECNKCIGFSGYNNYSDCSDCSDCSDLRLVAIPLNH
                     KNTAYKAAELFIKKYNIDCGVKIRLEKNIPVAAGLAGGSSDAAAVLTGMSRLFNCGED
                     ENELMCLGREIGADVPYCIKGGTALAEGIGEILTGLKNIPAAYIVLVVPRISVSTAWV
                     YENLDIRRINKRPDMKLLISAINEGNINMIGANMLNVLETVTAEKFGVINIIKEKLRE
                     YGAAGSVMSGSGPSVFGLFTDKKTAEDTYKSMAMYANKSTMFKNWDLFITETV"
     gene            6558..7241
                     /locus_tag="PK733_09775"
     CDS             6558..7241
                     /locus_tag="PK733_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ10868.1"
                     /translation="MANKLGKINLNDYKPLRDVIFTTLREAIIMGELKPGERLMEVQL
                     AEKMGVSRTPVREAIRKLELEGLVVMIPRKGAHVAKLTDKDIMDVLEVRASLDGLATA
                     LATQRINDEELKELKHVQSQFENYVEKGNLQGIIKKDIEFHDVIYRASKNEKLIQIVN
                     NLREQVYRFRVIYLKDFSSTEDVIKEHVEIYSAILERNIEAARESAKRHIRNQEQVIV
                     KAIKKSELN"
     gene            7387..8136
                     /locus_tag="PK733_09780"
     CDS             7387..8136
                     /locus_tag="PK733_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ10869.1"
                     /translation="MINAIIVAGGQTIKGLEEAGNKALYSINGRAMIEYVIDAVRMAD
                     DVQKVIVVGPKNSLDKHLKGKIDALIDSDGSVMENVMAGISYLGDDNYILICTCDIPL
                     ITVEAVNDFITKAKVLGADLCYPIVEKELNDKKYSGMERTYVKIKEGQFTGGNIFYVN
                     PKTVKENYSFAEKLIVYRKKPIKMARLLSFNFMIKFLMGKVTIDMVEKKFSEITGISG
                     RAVISLYPEIGQDVDKSCDVYAATGYLNSRE"
     gene            8155..8535
                     /gene="acpS"
                     /locus_tag="PK733_09785"
     CDS             8155..8535
                     /gene="acpS"
                     /locus_tag="PK733_09785"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOJ10870.1"
                     /translation="MGISCGIDIIEVDRIRGAMEKRGQAFKERVFTENEIIYCEMKKA
                     SKYHSYAARFAAKEAVSKALGTGIGRGVDWKDIEVLNDDYEKPFAVLTGSANDYYNSM
                     KAKSMSLSLSHCKNYAVAQVVIET"
     gene            8553..9713
                     /locus_tag="PK733_09790"
     CDS             8553..9713
                     /locus_tag="PK733_09790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOJ10871.1"
                     /translation="MKNIKFLHCADIHLDAPFSSLGPNDGKSSIRRQDLKDTFLSIIK
                     IAKKEDVDFIFICGDLYEHNYIRKSTINFINGIFRTISHIRIIIIPGNHDPRVKGSYY
                     KNSDKYFEWAENVYILTDDMPYIEFKEKNVCIYRYVNQQALSVDKEKINILLAHGTVD
                     FNFDKNSYNPVTSGQLASTGMDYIALGHFHNRMDNIGDRGNIFNPGSPEPLGFDEPGE
                     HGVFIGSITKDRLDVRDLDVRFITVNKKEYIDINVDITICATDEQIAEKISSALANHA
                     DNANYIKKVSDLLVSVTLTGYIDRQLTLDVEYITSLLKETFFFIKIKNETLPDYDFDE
                     IKKETGLKGLFARKIFDLIDRTDNVNEKKLLMKSLYYGIEALEKGQVDIFGY"
     gene            9703..12012
                     /locus_tag="PK733_09795"
     CDS             9703..12012
                     /locus_tag="PK733_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ10872.1"
                     /translation="MVIKRIGIKGFGKIKDLEIKPIDGFNIIYGENEAGKSTIQWFIR
                     GMFYGLKGGREKEGVLPPLKSLSPWNSGEYSGFIEYCMSNGEIYTVSRNFVDNSVRIL
                     DSSFNDITDTFEIGKDKSVRFAEQHLGLSEVCFEKTVFIKQMETKIDGNAKDELLNKI
                     MNAGETGFEDVSFKKAEKALKDALITYVGTDKTTVRPLDKVNQRLKELKNTFNELDEE
                     RCKLVEVETQLGEALLLMDKYRTEKGSLEILTEVAAIAKIINENKRIQLELDEIYEGL
                     LKVSNEKREITSEITGLENTTNQKRGQWTCCSKGQECFYDSKPEYNMQLECNVPFKYD
                     FQEVITLKDNIDNAKYEYNKSISDIKASEQELNKKKKRKKLADIFFITSLIASFIFSC
                     GAAYLFLNSLKIMNLPGITATVALLSWIVSIVLFCIRKNINKAFNNTIEKVNSFKSYS
                     ETLKVNLEQYREKLLNLKELIQLKERHEDLSNRIQYYLKRASFLTGVNILNAGNIQSE
                     IANIQAQTSELEASLKPLPGLEEETIKIKEMKTEKIEQFELDLKQKSSSLNQNMADLA
                     LNIKEYETIIDASSYDNEQLQKITEEIDALESERERLLNLRFTLTKALDVLTEASNEI
                     KKDYIPLLNEKMSCYIRKITDEKYADLRIDDKFALKILGPETPMVVPALQLSGGTADQ
                     MYLALRLALSDIFSYGGEKLPLIMDEVFAQYDDTRVEAALKMMNELSRERQIFLFTCK
                     KREVELAEKYFGNMGTVLSIEDDLQPLIL"
     gene            complement(12002..13135)
                     /locus_tag="PK733_09800"
     CDS             complement(12002..13135)
                     /locus_tag="PK733_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ10873.1"
                     /translation="MNILFLSVSTGGGHIKAAKALEETVVSKYSGAKCLIIDTLKYIS
                     PFLDRMIVGSYLGIVRNIPEFYGRLYSLSEHNNRINNITINLSRLLSKYLISLIKDFT
                     PSVIVCTHTLPLQMVSFLREKKIISVPVVAVVTDYVNHPFWNLEYVDALIVPGNHVMD
                     DMVKAGIPESIIYPYGIPLSENFLNKWEKAEIRKALGIENKLTGLIMGGSLGIRSVYN
                     PFKSLMNCMNCKPDFQIIVVAGLNSKLQRKIERDLLNYPETHHNRVKVLGYANNISDL
                     MDASDFIITKAGGMTISEALIKELPIFLMSPIPGQEERNTTFLVNSGAAVEISPRDDI
                     GDVLGKTIYIPEKLTMMKEKAKALACPYACYDTVKLLESLSIR"
     gene            13570..13923
                     /locus_tag="PK733_09805"
                     /pseudo
     CDS             13570..13923
                     /locus_tag="PK733_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
     gene            14034..>15294
                     /gene="ypeB"
                     /locus_tag="PK733_09810"
     CDS             14034..>15294
                     /gene="ypeB"
                     /locus_tag="PK733_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HOJ10874.1"
                     /translation="MGFREKLQDFKRRLSDRKMYSIVIVVIAAITIWGIYQYKHAANL
                     RQELDNQYNRSFYDMIGYVNNVETLLLKSLITSTPEKTASILQEAWRQSNFAQAYLGQ
                     LPVSQHVLANTSKFLSQVGDYSYSLNNNTMGGKKISDKDYKTIEDLHGYAAALGKSLG
                     TLQADLASGRIKWGELSQKGPSVFQKASASLTAQKFENIDKTFQEYPTLIYDGPFSDH
                     LVTAEPKGVTGDVLSAENVKEKVADFFGRDKVASMDNTGKNDSGPIKTYSYVVKFKGA
                     PDDQTASIDFTQKGGHPFWMIYNRPVNEKKIDVDKAKGIGREFLAKHGFKDMADTYYL
                     SEDNTAIINYAYQQNDVIVYPDLIKLKVALDTGEIIGFEAKGYLSAHTVRDIPKPQIT
                     MEEARTKINTKVSISSSGLAIIPTDFKT"
BASE COUNT         5578 a         2143 c         3082 g         4491 t
ORIGIN      
        1 tggaaattta tcctgtaaag aaaaatttgc taaaagaaac tacaaaaaaa agaatggcta
       61 atacttttca attatttaaa aaagcagctg gggagcaaac aactataccg tatcatggcc
      121 aggaaattga tgaggattat attcttattc aaaatatcaa agatgccaaa aatgaatggc
      181 taaatgcgga tttaaacctc caatatgtaa atgaaaccga atttgttgaa tattacatat
      241 acaagctaaa agccgcacaa ataaaatatg aattcttttt aaaaaaagca aaggaaagag
      301 gcttaaaaat aagaatagac acaggtatac aataaaaact taatttaata gtaataaatt
      361 cttggccaag tatatatata ttaaacaagc atatattttt atttattttg aggagattgg
      421 caatggctcc gttaaagata atttttaagc ttgttggtaa agtaataatt ggaggaattg
      481 ttctcgtttt aattaatatg gttggcaggt tatttggctt tcatattagt attaatgtta
      541 tttctgcttt tgtcacaggg acacttgggg ttcctggagt gatacttatc attgccctta
      601 aatcaatttt ccggttttga gaccatctaa gtaattggcg atcttaatat cctgctatct
      661 gatactatct gagtatttgg gctaaataat gactgaattt tgggagaact gtccccttct
      721 gagcccttct gagctattac ctgttaagtt aaagagaagt gcgtcttccc aggttatttc
      781 gtccttaatt taaaatatgt ctcagtattc tacgtattaa ttagcaatta tcttgcatat
      841 atttatatac gggagaaaga ttatagtaac taactactgg agggcgctta tgtacgatgt
      901 tatagatagt attattgttt caaaatatgg cttgaaaata aaaagctcaa taccttttag
      961 agaagggttt attttagcaa catcaaaggg aagatttcta tgcaaggaaa ctcgtatgaa
     1021 agctggcagg atattgtttg tacacagcgc taaggaacat ttagtgaata atggctttaa
     1081 aaacctggat cgctatttat gtacccaaga gggggaacct tatgttataa acaatgggaa
     1141 atgctatatt gtttcatata ttattgaggg taaagaatgt gattttgaaa atagaaatga
     1201 tgttaaaagg gcctctaatt tgcttgcctg catgcatgcg gcatcaaaag ggtatattcc
     1261 gcctgcaggc agtttaataa gagatgatgt tggtaaaact ccatctttat tttgtaaaag
     1321 actaaaggaa attaaaagga tggaaaaaat agcatctagg agaaaaagca attttgatta
     1381 tatatatgcg gcttattcca actattttta tgaagtggga aaagatgcct tggattatat
     1441 tttaagtcca atatacgata agcttgttaa tgaaaccagg ttggaaaaga ttttttgtca
     1501 caacgattta acatacagta acctgatttt gaacgacaac agagattcat taattaattt
     1561 tgatttctgt tgttatgacc taaaagttta tgacttagcc aacctcatca gacggagaat
     1621 gaggagatgc aactgggata ttcgtgaagc aaaagtaatt acagatgaat ataatagtgt
     1681 aaataaactt acacctgaag atttctatgt catgaaaata atgctgcaat ttcctcaaaa
     1741 attctggaga gtggcaaata aatattataa tagtaggaga agctgggctg aaagagcttt
     1801 aactgacagg ttggaggatg taattaatga aatagattgt cataaaaaat tccttgataa
     1861 ttatgaaatt attatataag ttacagtatc aatcacatta aacacccact cgcatattat
     1921 gtaatatgag tatgaagtat aataagatgg gtgggaaata tggggaatgt aaaagtcggt
     1981 gatattgtag caagaaattc ctatggatgt gatatatttt ttaaagtagt agaaataaaa
     2041 agtgatggaa aacaaaacat cataactcta aaaggcatta cttatagaat acaggcagat
     2101 gctccagaat cagatcttat agtccaatcc gatgcaaatg taagagaata tagaaataaa
     2161 gtggatacag aggcatatag aaaattaaag cagacagaat actattcagg ccggatggga
     2221 aatagagtgg cgtggccaaa aaaaggtttt catagagata cttccaatga cgacacccgt
     2281 acttttacaa ggccgggtaa aatacttcat tttgatgggg atgaggatta tcttgaaaca
     2341 tgcattaaag aatatataaa gcgtggagtg gatgcggtag gaaagcatat tgctgaaaag
     2401 gaacagccat cgcgtatata taagcttctc caggaatata agcctgatat acttgtaata
     2461 actggccatg atagtatttc aaaagaccaa aaggattatt taaatataaa ctcttatgtt
     2521 aactcaagat attttatcga atctgtaaag gaggctagaa ggtataaccc ggatatggat
     2581 agtctggtta tttttgcagg agcatgccaa tctttgtatg atggaattat aaaagcagga
     2641 gcaaactttg caagttcacc ggatagagta ttgattcatg ctttggatcc tgtgcttgta
     2701 gcggaaaaat tatcccttgc accaatagat agagtagtaa tgcctatgga tgtcataaat
     2761 aatacaataa cgggcgcgac gggaataggc gggcttcaga caagaggaaa attcagaaaa
     2821 ggatatccca aagaaccata tacaggtaat atttgacccc aaacccatat aacgggaata
     2881 ttgaatatat gaaagtaact atatgttatg gttcatataa aatagaattt ttaagttaaa
     2941 tatataattt atttttaata ttgcgtgtat ttcttggatt tacgccttaa aatggtactt
     3001 gacagcacat aatattaact gttataatat ttaacttttt tttgactttt gtaccaacat
     3061 gtgatataat aaatacgaac tatgaagcaa tcagattggc ggattgcatt gatgttataa
     3121 taaatacaaa taacgaaata aaacctgatg gctttacttt tatgtattta ttgaatgatg
     3181 tgaagcaacc ctattagcgg attgcactta tgttataata ttaacaaacc gaagatgagg
     3241 tgatgtaatg gcagaaaaaa gtgatctact tgaaataaag agaaatattg aaacttgcat
     3301 aggtgaaaaa gtacagttga aatctaacaa gggcaggaaa aggaccttta taagagaagg
     3361 tgttattgaa aacacctacc cgagtatttt tacaataaaa tttgaaaatg agtatgatat
     3421 gaccagaaga gtttcatata gttatacaga tgtccttaca aacgctgttg aactcattgt
     3481 atgcagaaac aaccgcagga tacaggtaag ttaaatatca taatcctcat ttataaatat
     3541 ttgttattaa tatcctgcat taagcaacca taaatttaaa aaggttgctt ttctttctcc
     3601 tttttttcta tcattttgat ttatataatt taaaagtaat attttataca ccttgtcaat
     3661 atatattatt atagttaagg ttttgtttta tattctgata gataagaggt gggttggaat
     3721 gtctcttgat ttagctagag aagtaataaa ggtaagcaat ataattaatc gtgaaattac
     3781 ccagattata gttgagaatg atattatagt accggataca aaaccggatg tagtgaaaat
     3841 actgattgcg gaggcagagg cgtatactac aagtgcagta gttaatacag atgaaaaaag
     3901 tacctttatt aagggaatta tacgttataa aatactttac aaaccggatg atcctgatat
     3961 agatattaaa agcataaata taaatgctga attttcccat gagatggata ctcctgtggc
     4021 cggtaaggat gtaagaaata ttgtaaagtg tttggcagag catgtagaat gtgaaattat
     4081 caatgggcgg aaaataaaca ccaaaaccat attacaatta aactcaaagg ttatggctga
     4141 ggaagagtat gttttaataa atgatataag tgataatggt gacattcagg tacttagaaa
     4201 caatatggaa ataaacaatt tcataggtag cagtgaagca ttatatacaa ttaaagaatc
     4261 catggaagtg cctgagggca atcctcccat tagggaaata ttgagtaatg atgttaaaat
     4321 tatcaacaaa gatttcaggc tttccgataa taaagtcatt gttacaggtg agatgaatat
     4381 tgtcacatta tatgcaggag atgatcagga gtcaagtata aggttaatgg aacatgcaac
     4441 tccattcacg cagttcgtag atctgtctgg aattaatgat gaatctgatt gtgaaatagg
     4501 ttatgaagta agggactata gttttgaagc tgcagaagat gctgatggag aattaagggt
     4561 aatgaatatg gaagttgagg tatctattaa tgtatatgga agtgtaagaa aaaatattga
     4621 tattgtacat gatgcctatg gactgagatc tggagtaacc cttgataaaa aggtagtaaa
     4681 aacggaagaa aaggttacag aaaatatttc ccaggcagtt ttaaaagaca ctgcaaccat
     4741 aggagaaggg aaccctgaaa ttgttgagat aataaatatg gtcagcaaac cggttttaac
     4801 ggattataaa gttcaggaag ataaaattga aatagaaggt tttgttgcta acaatttaat
     4861 ttatttatca aataattctg agcaaaatat gtcatgcctg caaagtgata ttcctctaaa
     4921 acaaactatt gatattaaag gtgtaaaacc cggtatggct tgtgaaattg agcttctaac
     4981 agagcatgcc aattatagta tgctgtcctc gaaagatgtg gaattaagat tagtagttgg
     5041 tgtagtttct aaagtatata agaatgtcga aaaacacata attgaaaaag caatagaaat
     5101 tccatttgat gaaagcagga aagctgataa aaaagccggt ataattgtgt atttcataca
     5161 accgggggac actctctggg aaattgcaaa gaaatatacc acaacggtta acgatataca
     5221 gagagttaat atgtttcaag atcaggattc gatcgatgat ggaatgcaaa tacttataac
     5281 tcgcaaatcc taaaaggttg caaatgggaa gcagtttaaa atgggggacg gttctcaact
     5341 gaactactga gaaccatctc ccattttttc aagggtgcat ttacagtacc ttcactaatg
     5401 cttctttcaa taatttcttc gaaaataaaa tgtgaaccct tttgtaaaaa aattgattga
     5461 aagatacaac ttaataatat ataatgtata caaattgagc agcacttgat ggtatttcag
     5521 aaaattaaaa agagcgggtg tgcaacaaaa tatgcagcta aatatgcaac aaaatataat
     5581 ggacttctgc ccataccgta caattagtct tatggccagg gcaaaagtta atttagccct
     5641 ggatgtaacg gcaaaaagac tggatggtta tcatgaaata aaaacaataa tgcaaacaat
     5701 caaactccat gataaagtaa ttattgatgt tattgacaaa ggtatagaaa ttgagtgtaa
     5761 taaatgcatt ggctttagtg gttataataa ttatagtgat tgtagcgatt gcagcgattg
     5821 cagcgattta cgtttagtag caataccgct taatcacaaa aatactgcat ataaggctgc
     5881 agagctgttc attaaaaaat ataatattga ttgtggtgta aaaataaggt tagagaaaaa
     5941 tattcctgta gctgcaggtc tggctggagg aagctccgat gctgctgctg tactgacagg
     6001 aatgagcagg ttattcaact gcggggaaga tgaaaatgaa cttatgtgcc tggggcgtga
     6061 gataggagca gatgtacctt actgtatcaa aggtggcaca gcattggctg aaggtattgg
     6121 ggaaattctt actgggttga agaacattcc agcagcttat attgttttgg tagtaccccg
     6181 aatatcggtt tctactgcct gggtatatga gaatttagat attagaagaa taaacaaaag
     6241 accagatatg aaattattaa tatcggccat taatgaagga aatattaaca tgatcggggc
     6301 caatatgcta aatgtattag agacagttac agctgaaaag tttggtgtta taaatattat
     6361 taaagagaaa cttcgtgaat atggggcagc gggaagtgtt atgagtggca gcggaccttc
     6421 tgtgtttggc ctattcacag ataagaaaac tgcagaagat acgtataaat caatggcaat
     6481 gtacgccaat aaatctacta tgtttaaaaa ttgggattta tttattacag agacagtgta
     6541 agaggagata ggataaaatg gcaaacaaac ttggtaaaat aaatttgaat gactataaac
     6601 ctttaagaga tgttatattt accacactaa gagaagcaat aataatggga gaattaaaac
     6661 ctggtgaaag gttgatggaa gttcaactgg cagaaaaaat gggagtaagc agaactccgg
     6721 tcagagaagc tataagaaag ctggaactgg aaggccttgt tgttatgatt cccagaaagg
     6781 gagctcatgt ggccaaactc actgataagg atattatgga tgtacttgaa gtaagagctt
     6841 ctcttgatgg acttgctaca gctctagcta cacaaagaat caatgatgaa gaattaaagg
     6901 aattaaagca tgtacagtcc cagtttgaaa actatgtgga aaaaggtaat ttacagggta
     6961 ttattaaaaa ggatattgaa tttcatgatg taatatacag agcatcaaaa aatgaaaaac
     7021 ttattcagat agtcaacaat ttgagagagc aggtatatcg ctttagagtc atatacttaa
     7081 aagattttag cagtactgaa gacgtgataa aggaacatgt ggaaatctat tctgctattt
     7141 tggaaaggaa tatcgaagca gcaagagaat cggcaaaaag gcatataaga aatcaggaac
     7201 aggttatcgt taaagcaata aagaaaagtg aactaaatta aagcatattt cttgcaaaga
     7261 aattttgaaa aaaataaatt gcatgcgcgg ctaaaatgaa tttaaatcta taaaaacttt
     7321 ttcatgaaaa agttatgttc ctgcaaaggc gaattcattt cgcacgcagc agaaatggag
     7381 cttaatatga ttaatgctat aatagttgca ggaggacaga caataaaggg tttggaagaa
     7441 gccggaaata aagctctata ctcaattaac ggaagagcta tgatcgagta tgtaatagat
     7501 gctgttagaa tggctgatga tgtccaaaag gtaattgtag taggcccgaa gaacagcttg
     7561 gataagcatt taaagggtaa aattgatgcg ctgatagatt cagatggttc agtaatggaa
     7621 aatgttatgg caggaataag ttacctgggt gatgataatt atatccttat ttgcacttgt
     7681 gatattccct tgattacagt tgaggcagtt aatgatttta taaccaaggc aaaggtattg
     7741 ggtgcggatc tgtgctatcc aattgttgaa aaggaattaa atgataaaaa atactctggc
     7801 atggaaagga cttatgtaaa aataaaggaa ggacagttta caggcggaaa tatattttat
     7861 gtaaacccaa agactgttaa agaaaattat tcttttgcag aaaaacttat agtataccga
     7921 aagaagccga ttaaaatggc taggcttcta agctttaatt ttatgataaa gttccttatg
     7981 ggaaaagtta caatagatat ggtggaaaaa aagttttcag aaataacagg aataagcgga
     8041 agggcagtta tttcccttta ccccgaaatc ggacaggatg tcgacaagtc atgcgatgtg
     8101 tatgcagcga cagggtatct taactcaaga gaatgaaggg taggtgtaaa gcgcttggga
     8161 attagctgtg gtattgatat tatagaagta gacagaatac gcggggctat ggaaaaaagg
     8221 ggacaggctt ttaaagaaag ggtttttact gaaaatgaaa tcatatactg tgaaatgaaa
     8281 aaagcttcca agtatcatag ttatgctgca cgttttgcag ccaaagaagc tgtttcaaag
     8341 gcactgggca caggtattgg aagaggagtt gattggaagg atattgaggt cttgaatgat
     8401 gattatgaaa aaccttttgc agttttaacc ggtagtgcaa atgattatta caattcaatg
     8461 aaagccaaga gcatgtcttt aagcctctca cactgtaaaa actatgcagt agcccaagtt
     8521 gttattgaga cataaggaga ggaaagtata gattgaaaaa tattaaattt ttacactgtg
     8581 cagatattca tctggatgct cctttttctt ctctggggcc aaatgacggg aaatcatcta
     8641 ttagaaggca ggaccttaaa gatacttttc taagtataat taaaatagcc aaaaaagagg
     8701 atgttgattt catatttatc tgtggtgatc tgtatgaaca taattatatt cgaaaatcca
     8761 caataaattt tattaatggt atatttcgta ctataagcca tattagaatc attattattc
     8821 ccggaaatca tgatccacgg gtaaaaggct cctactataa aaatagcgat aaatattttg
     8881 aatgggcgga aaatgtctat atacttacgg atgatatgcc ttatattgaa tttaaggaga
     8941 aaaatgtttg tatatataga tatgtaaacc aacaggcttt atcagtggat aaagagaaaa
     9001 ttaatattct tcttgctcat ggcacagtag attttaattt tgataaaaac agttataacc
     9061 ccgttacaag cgggcagctt gcttcaacag gcatggatta tattgctctg gggcattttc
     9121 acaatagaat ggataatata ggtgacaggg gcaatatatt taaccctggg agtcctgaac
     9181 ctcttggatt tgacgaaccc ggagaacatg gtgtttttat tggaagcata actaaagacc
     9241 ggttggacgt gagagacctt gatgtaaggt ttattacagt aaataaaaaa gaatatatag
     9301 acatcaatgt ggatataacc atatgcgcta ctgatgagca gattgctgaa aaaatatcaa
     9361 gcgctttggc aaaccatgct gataatgcaa actatattaa gaaggtgtca gatcttctgg
     9421 taagcgtcac tctcaccggt tatattgaca gacaattaac tttggatgtt gagtatatta
     9481 cttcgctctt aaaagaaaca ttttttttta taaaaataaa aaatgaaacg ctgcctgatt
     9541 atgattttga tgaaataaag aaggaaaccg gattaaaggg cttatttgca agaaaaattt
     9601 ttgatttaat agatagaaca gataatgtta atgaaaaaaa gcttttaatg aaatctcttt
     9661 attatggtat tgaggcatta gaaaaaggac aggttgatat atttggttat taaaagaata
     9721 ggaataaaag gttttggtaa aataaaagat cttgaaataa agcctataga tggctttaat
     9781 ataatatatg gagaaaatga agcaggtaaa tcaactatac aatggttcat taggggtatg
     9841 ttctatggtt tgaagggtgg gagagaaaaa gaaggtgtct tgcctccact gaaaagtttg
     9901 agcccgtgga atagcggtga atactcaggt tttattgaat attgcatgag taacggagaa
     9961 atatacacag taagcagaaa ctttgtggat aactctgtaa ggattctaga ctcatctttc
    10021 aatgatatca ctgatacttt tgaaatagga aaagataaaa gtgtcaggtt tgcagaacag
    10081 cacttaggtt tgagtgaagt ctgctttgaa aaaactgttt tcattaaaca aatggaaacg
    10141 aagattgatg ggaatgccaa ggatgaactt ttaaataaaa tcatgaacgc aggagaaacc
    10201 ggttttgaag acgtttcatt taaaaaagcg gaaaaagctt taaaagatgc ccttataact
    10261 tatgtaggta cggataaaac tactgtccgc ccattggaca aagttaacca gcggcttaaa
    10321 gaactgaaaa atacttttaa tgaattggat gaagaaagat gcaagctggt tgaagtagag
    10381 acacaacttg gtgaagctct gttgttaatg gataaataca ggactgaaaa gggatcattg
    10441 gagatattaa cagaagtagc tgctatagca aaaattatta acgagaataa aaggatacag
    10501 ttagagcttg acgaaatata tgaggggttg ttgaaagttt ccaatgaaaa gagagagatt
    10561 acttcagaaa taaccggact ggaaaatact actaaccaaa aaagaggaca atggacatgc
    10621 tgtagtaaag gccaggaatg tttctatgat tcaaaaccgg aatataatat gcagcttgaa
    10681 tgtaatgtac cattcaaata tgattttcag gaagttatta ccttaaaaga taatatagat
    10741 aatgccaaat atgaatataa taaaagcata agcgatatta aggcatctga gcaggaatta
    10801 aataaaaaga agaaaagaaa aaagttggct gatattttct ttataacatc gctgattgcc
    10861 tcatttatat tctcctgcgg agctgcatat ttatttctga actcactgaa aatcatgaat
    10921 ttgcccggta taactgcaac tgttgctctt ttatcatgga tagtttcaat tgtattgttt
    10981 tgtattagaa agaatattaa caaagctttt aataatacta tagaaaaagt aaactccttt
    11041 aaatcatata gcgagacttt aaaagttaat cttgaacaat atagagaaaa gcttttaaat
    11101 ctaaaggaac tgatacaact taaggaaaga catgaagatt taagtaacag aattcaatat
    11161 tatctcaaaa gagcttcgtt cttaacgggt gtcaatattt tgaatgcagg taatattcaa
    11221 agtgaaattg ctaatattca ggcacaaacc tccgaactgg aagcaagttt aaaaccactg
    11281 cccggactgg aagaagaaac aataaaaata aaagaaatga aaaccgaaaa aatagaacaa
    11341 tttgagcttg atcttaaaca aaaaagcagc tcgcttaatc aaaacatggc tgatttagct
    11401 ttaaatataa aagagtatga aactataata gatgcttcaa gttatgataa tgaacagctg
    11461 cagaaaatta cagaggaaat tgacgccctg gaaagtgaga gagaaaggct cctgaatctt
    11521 cgatttactt taactaaagc acttgacgtt ttaactgaag caagtaatga gataaaaaaa
    11581 gattatatac ctcttttgaa tgaaaaaatg agctgctata taagaaaaat tacagatgag
    11641 aagtatgcag acttgcgaat agacgataaa tttgcactaa aaattttggg gcctgagaca
    11701 cctatggtag tacctgcact tcaacttagc ggaggcacag cagaccagat gtaccttgct
    11761 ttgaggcttg ctttatctga tatattctct tatggaggag aaaagcttcc tcttataatg
    11821 gatgaggttt ttgctcaata tgatgatacc cgagtggagg ctgcccttaa aatgatgaat
    11881 gaactatcca gagagcggca gatcttttta tttacatgca aaaaaagaga ggtggagtta
    11941 gctgagaaat attttggaaa catggggaca gttctgagta tagaagatga tctccaacct
    12001 cttatcttat agacaagctc tccagtaact tcacagtatc ataacaggca taaggacatg
    12061 ccagcgcttt tgccttctcc ttcatcattg ttagcttttc aggaatgtaa atagttttac
    12121 ctaaaacatc acctatatca tcccgtgggg atatttccac agcagcaccg ctatttacaa
    12181 gaaaagtagt gtttctttcc tcctgacctg gaataggaga catcaagaat atggggagct
    12241 ccttaataag tgcttcagat atggtcatac cgcctgcttt agtaataata aaatctgatg
    12301 catccattaa atcagaaatg ttatttgcgt atcctagtac cttaaccctg ttgtgatggg
    12361 tctcaggata atttaacaaa tccctttcaa tctttcgctg caacttacta ttcaagcctg
    12421 caactacaat aatttgaaaa tcaggtttgc aattcataca attcataagt gatttaaaag
    12481 gattgtaaac cgatcttata ccaaggcttc ctcccataat aagacctgta agtttatttt
    12541 caattccaag tgcttttctt atttctgctt tctcccactt gttgagaaaa ttttcagaaa
    12601 gaggaatacc atatggatat attatacttt caggaatacc tgcttttacc atatcgtcca
    12661 ttacatgatt gcccggtact ataagtgcgt caacgtattc aagattccaa aaaggatgat
    12721 taacataatc tgttactaca gcaactacag gaacagaaat aatcttcttt tctcttagaa
    12781 aggataccat ttgcaacggg agagtatgag tacatacaat aacagaagga gtaaaatcct
    12841 tgattaatga aatcaggtac tttgataaaa gacggcttaa attaatagtt atattattta
    12901 ttctgttgtt atgctccgac agactgtata atcttccata aaattctggt atattcctta
    12961 caataccaag ataactgcca acaatcatac gatctagaaa ggggcttata tatttaagag
    13021 tatcaataat aaggcatttt gcgcctgaat attttgatac aacagtttct tccaaagctt
    13081 tagcagcttt tatgtggcct cctccggtag aaacggataa aaacagaata ttcattctac
    13141 accaccattt aataaatttt ttttcgtcgt atataattat ttgcagtaat acgatagttg
    13201 atacaaagta tgtgcatatt ttcttattaa atacaaaaaa tataatttga agtcgtgaaa
    13261 gtagaaattg aaaaatcata atcaaggtta aaaccaaggt caggattatg gtcaaaatta
    13321 aggctgaaat taaggctgaa attaaggctg aaattaaggt tgaaatcaac ttatagtcat
    13381 ggggaggagg aaattataag attgaaaagg tttaaattaa tcactttatt tattatagtt
    13441 attgtttcta cattttttgt tcttaactat gatacaggca ctatttcaaa agctgctgaa
    13501 agattaattc cgggaatcgg tgaaatgttt aagagagttg ttgtatatgc tcagggacag
    13561 ggccagacta gtgaagaaca gctgcttgcc agggctataa acggtgaagc taggggagaa
    13621 ccctatgaag gccaggtagc agtgggagct gtgatattaa acagaacaag acatccgagc
    13681 tttcctaaaa ccatagcagg agtaatatat caaccgggtg catttactgc ggtttctgat
    13741 ggccagatta atgtggctat agaccctaat tccagtgttg taaaggcagc aagagatgca
    13801 ttaaatggat gggaccctac aaatggatgc atatattatt ggaaccctgc tacagcaacc
    13861 agtaaatgga tatggagcag gcaaataatg aaaacaatag gcaaacatca ttttggaatg
    13921 taagtggtat ttagtactac tgtaccaatt tggaaagaaa atgccgtaat attatttaaa
    13981 atgcggcggt attaatattt taagaattat aagaatttaa tggaggtgta accttgggat
    14041 ttcgagaaaa actgcaggac tttaaaagaa gactcagtga tagaaaaatg tatagtattg
    14101 ttattgtggt tatagctgca ataacaattt ggggtattta tcaatataag catgcggcta
    14161 atttacgcca ggaattggat aatcaatata atagatcttt ctacgatatg attgggtatg
    14221 taaataatgt tgagacttta ctgctaaaat cattaataac ctcaactcct gaaaagacag
    14281 cctcaattct ccaagaagcg tggagacagt caaactttgc tcaggcctat cttggtcaat
    14341 tgcctgtttc acaacacgta ctggcaaata cctccaagtt tctttctcag gtaggtgatt
    14401 attcttattc tttgaataac aataccatgg ggggaaaaaa aatatccgat aaagattaca
    14461 aaactattga agatcttcac ggatatgctg ctgctttggg aaaaagcctt ggtacgcttc
    14521 aagcagatct ggcttcaggc agaattaaat ggggggagct ttctcaaaaa ggcccttcag
    14581 tatttcaaaa agcttcagca agtctcacag cacaaaaatt tgaaaacatt gataaaacat
    14641 tccaggaata tcccacactt atatatgatg gaccattttc ggaccacctt gttacagcag
    14701 agccgaaagg tgttacagga gatgttttaa gtgctgaaaa tgttaaagag aaagttgctg
    14761 acttttttgg cagggataaa gtagcgtcta tggataatac aggtaaaaat gatagcgggc
    14821 caattaagac ttacagttat gtagttaagt ttaaaggcgc tcctgatgac cagacagctt
    14881 caattgattt tacccagaag ggaggacatc ctttctggat gatttacaac aggccggtga
    14941 atgagaaaaa aatcgacgtt gacaaggcaa agggtattgg cagggaattt ctcgcgaagc
    15001 atggcttcaa ggatatggca gatacctatt acttaagtga agacaatacg gctataataa
    15061 actacgctta tcagcaaaat gatgtaatag tatacccgga tcttataaaa ctgaaagtgg
    15121 ctctggatac aggcgaaata attggttttg aagctaaagg atatctttcg gcacacactg
    15181 ttagagatat ccccaagcct caaattacaa tggaagaagc cagaacaaaa ataaatacaa
    15241 aggtttcaat atcgagttcc ggtctggcca ttatacctac tgatttcaaa actg
//
LOCUS       DAOSDD010000095        15060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1581_length_15060_cov_5.694169, whole genome shotgun sequence.
ACCESSION   DAOSDD010000095 DAOSDD010000000
VERSION     DAOSDD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15060
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1581_length_15060_cov_5.694169"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK733_09815"
     CDS             complement(<1..656)
                     /locus_tag="PK733_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10875.1"
                     /translation="MLPFHGNLIKAENGPLNWQEIDIKISADTWYNFMLHFKADNTFE
                     IYINGKQARIAGSYSGQRIAPGKRMNAIGYIGDIRSSYHDGCAYFDNFKVYTAEDIIF
                     EEGFNNGNLDGWEIYGSTDLADPAVDCPKVNLESAAINTSTTGLLIGASTNISLSGLF
                     SNGKAVDLAISEKIDIQSSDESVAKVEYNDGVPAIVGVGKGTTQITASIRYKGEVATA
                     S"
     gene            complement(586..2049)
                     /locus_tag="PK733_09820"
     CDS             complement(586..2049)
                     /locus_tag="PK733_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ10876.1"
                     /translation="MGKFKLGNFTRRNILTYLLVFIFLFVNLSQIVTSAQNVIQYENK
                     FYVSINGDNNNDGSIESPFRTITHALAVIPSNSELVIRGGTYTDEAAVLSGKSGIVIE
                     NYDNEIVSIINQAEKYFSVSKCSDITIKGINIAYSGGRSKNTLIVSDSSNINISHVHS
                     TGGDVLLFKVDTFKLYSSTFSAAQGDSGATVRDTKNGKVYNNIVYNNTNYGIYVLGNS
                     SNNSIYNNTLFNNAKGDIYILSHPTLGTPQNNRICNNIITRGLYLLSMNEADIVGLNQ
                     FNYNAYNENLAGNIGIISPTHPDGISFTDFKALPSAPEQNAVIGNISFIDSSINDFRL
                     KGDSICIGNGTPADAPTHDYRGLERITGVDIGAYQFSEDDIFRLAIVSYSPQGQSNGI
                     DVPVVVSFNNNISADNIDGLIKVVDPQGNEISGSISVEGKTITFTISAFLEYETKYSV
                     TVDGNIKDIDGNVLGAPFSWEFNKSRKWTIKLARDRH"
     gene            complement(2287..3939)
                     /locus_tag="PK733_09825"
     CDS             complement(2287..3939)
                     /locus_tag="PK733_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10877.1"
                     /translation="MKQKFIKFTALMIAVLMAMSLFAGCVVKDSSKDNTTPKSESAKD
                     NKAANEEKDKYQPIDGKVYEITYLTMTNAPVNEDSYIGRKFNEMFNVKLKPVFVESSK
                     WDDLLNIRLSSGDIPDVFPSKSSDRFLRYQHGELIQELPIEVINKYAPDMLKAISRDD
                     PKSWSVCTFEGKNYGIPYLVANYMIRDAIVWRDDWLRNVGIEKIPDTITEWEEALIKF
                     RNDDPDKNGNKDTYGCSGTIIGPVFGAYGAMIDMWWNTFWLEKDGKIVNSAIEPGTKE
                     ALKLLSRWYGMDLIDPEFVAGENKGGYWAVSTDFVNSKIGVSTMGSAYHWQKEGNGLV
                     GGANYNEMKKINPDATYEFGNPPLGPDGKSRGTTKLPLSTGASYVFSKNVEPDKLGRI
                     LQMFNWPMADYKNYIFNRYGEEGIHHIVTKEAGYDVYNIAEPYKSDPARATGEVGYAA
                     FFWQADVNNTRKINGAYFDNFLKKGFDRYGIEDMGYKLYIPSGATFATDLHSLMQETY
                     INIITGKKPIESFDEFVEKWKKNGGDQMLKEANDHYAKTKSN"
     gene            complement(4061..4936)
                     /locus_tag="PK733_09830"
     CDS             complement(4061..4936)
                     /locus_tag="PK733_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10878.1"
                     /translation="MSTVKRSSGEMVFDVANAICLIIFSLLCIYPFLYVFNISVSKGA
                     SSAQYGLNLLPKGFTLEYYKQLFRTDAILTGLKNSVLRTISGTFLAVLVTSCGAYPLS
                     KKYLPNRKFWTTIILIPMFFGGGLIPTYLLIKSLRLIDTRWVLILPGLVSTYNLILIR
                     NYMISLPESLEESAKIDGANDIIILFRIILPICKPILATVALWVAVGHWNAWFDSLLY
                     ITKKELVVLQVVLYQVLNWGSERTVTGTMDNYNPAQEIIKACTIMVTTIPIILTYPFL
                     QKYFIGSIMVGSVKG"
     gene            complement(5010..6005)
                     /locus_tag="PK733_09835"
     CDS             complement(5010..6005)
                     /locus_tag="PK733_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10879.1"
                     /translation="METKTLNLNKTKEHGVLKQNSGIVILNKVINGLKKYKDNKYLFI
                     LLLPTLLYFGIFHYAPMYGIQIAFKDYKFSRGIWGSEWIGFEHFKLIFQGNNFLRVFK
                     NTLIISGLKFLFGFPAPIILAILLNEIRVSWFKRSVQTISYLPHFLSWVILSGIVKQF
                     LSPSIGPVGYVMNALNIKPIYFVVDPNWFRFVLVITSIWKEVGWGTIIYLAGMANVNP
                     EIYEAATVDGASRFQKAVKITLPSLVPIITFVFILSIGNIIHDDFDQIFNLMNPGVMQ
                     ISDVISTYTYRMGMVLMQYSYGAAVGLFKNVIAIALIFLTNYILKKISDNEYRII"
     gene            6585..8981
                     /locus_tag="PK733_09840"
     CDS             6585..8981
                     /locus_tag="PK733_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10880.1"
                     /translation="MYSGIKLPVRARNTVLIRFFLYFLMVILLTLTTISYFLYNYFSS
                     LFKDEVLSLNNRILSQVSDFSDAFILKNMNELAAGISMPYPHETFISQFFSDSDEDYW
                     KVILTTRNKLDSLIFQNRDIVDSIYLYSKDKKLALSQKVMKYIDEKEARNLDEFSWIN
                     QASMSNSSVIWLNTRIAKIYGSQSADNGNIITLICTYPVSSRPQDAKGYVAVNIREEA
                     LNNHLVKYNSTKLGHLLIVDKNGSIISHSDKSKLYNDISKERFVKEIMLSEKPKSFQS
                     KFENTDYIISFKQSAYNNWYYVAMVPTELLYQKDYHIKRKIFAASITIFFIVLLVSNV
                     FTYKMYKPLKKLLDKFASTINSEKKGKLDEYLMLENAFTDMSLKIDNLEDTLCKNVDM
                     IVHNYLIDLLNKPSIDNPNIGTTLNSLGVAFSEKYFTVIIFNISKAVVDLHKQTDIQL
                     CKYKIFEYIKMVDSTNTDNSYIDMDKGTDTNTKPICRYYPIDTDSRTISVIVNSNIND
                     LALVRNFITHTQFHCYNNFGFYLTVGVGKYFDNLLSVRQSNIDAKIAILYKFLHPGQN
                     EFYYEEISRKPANPEVLNQDYMEKLFKSLKLCNITEVNNILDTRISTISSNNFNYQYI
                     KKEISQLRKLYIECFEEMSVKIDSNSEIKDIFFYPGDVFEFFDACKVISNKFIDYMKE
                     KRTSKTSELIESVELYVLNNINGNLSLNLVADSLRISPQHLSKIFKEEKGINFIDYVT
                     DKKIDKAKNLLSTTNMSLDEISVCVGYSHATYFSRKFKESTGYTPGEYRQQEVKKQLK
                     "
     gene            complement(9150..10964)
                     /gene="ftsH"
                     /locus_tag="PK733_09845"
     CDS             complement(9150..10964)
                     /gene="ftsH"
                     /locus_tag="PK733_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ10881.1"
                     /translation="MKYLKGLSFYIVLFVIILFILALSQGTDNPVDMDYSDLIREIQN
                     NNVSEILLDNQKATVVLKKPYITDRVNRYVIYIPDLSSFMNEVSSPIKAQQIRFKTAF
                     PQTAPWWIAILPTVGLIVIFVLFWVFFLQQSQGGGGNRVMSFGKSRAKMSTDEKRKVT
                     FNDVAGADEEKEELEEIVEFLKQPRKFIELGARIPKGVLLVGPPGTGKTLLARAVSGE
                     AGVPFFSISGSDFVEMFVGVGASRVRDLFDQAKKNAPCIVFIDEIDAVGRHRGAGLGG
                     GHDEREQTLNQLLVEMDGFGENEGVIVLAATNRPDILDPALLRPGRFDRRIFVGLPDI
                     KGREEILKVHSRGKPLSQDVKLDELAKSTPGFTGADLENLLNEAALLAARKNKRKVEM
                     EEIKEATFKVIMGPEKKSRVMSEKEKKLTAYHEAGHAIAVKVISSTDKVDRISIIPSG
                     RAGGYTAFKPDEDRTYLTRGQLLEQISIALGGRAAEEIVLGEVSTGAYNDLKQANGVA
                     RNMITKYGMSENLGNLIFGNETDEVFLGRDFAHTRNYSEQVAAKIDNEVKKIIDTAYV
                     KIIDILKENISKLHSIANSLMEKEKLEGQEFEELFANT"
     gene            complement(11140..11739)
                     /gene="hpt"
                     /locus_tag="PK733_09850"
     CDS             complement(11140..11739)
                     /gene="hpt"
                     /locus_tag="PK733_09850"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOJ10882.1"
                     /translation="MGTNKSTNNAANVAVNNVTNRSEIKEVLVSREKIAEKVRELGQI
                     ISKDYEGKELVLIGVLKGAFIFLADLIREITIPVDMDFIAVSSYADGTKSSGVVRLIK
                     DMDVSISDKHVLIVEDIVDTGLTLNHLKNLFSTRGPKSVKICSIFDKPSRRLVDINVD
                     YKGIEIPNEFVIGYGLDFAERYRNLPDLCILDPNVYRHD"
     gene            complement(11801..13405)
                     /gene="tilS"
                     /locus_tag="PK733_09855"
     CDS             complement(11801..13405)
                     /gene="tilS"
                     /locus_tag="PK733_09855"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ10883.1"
                     /translation="MLNKGQNKLPDKLLGKVPVKVPDKIPGKVPDKVPDRVLDTIREH
                     DLIEPGDNILVGVSGGPDSVCLLHILYSFSHEMRIKLYAVHVNHMLRGEESDMDERYV
                     KELCESLGIQLFTVSLNVGDIAEKQNISIEEAAREARYGQFNTLADKIGNCKVAVAHN
                     RNDQAETIIMNIIRGTGLDGLRGMDYKRNRIIRPLLDIERKEIEEYCREHNLKTRTDS
                     TNLQSIYTRNKVRLELIPFISHNFYINIVESINRMADLIRDDYSYMDLTADEEYKRCL
                     LYDKNGDEGNYIKNVKNMVNLDIRRIRSLHNAIRRRVIRKAVYSVRGNLKCIESIHID
                     KILELCLDGRTGAEIHLPFGIRAAKSYDILKIYVNNNYKNELNNPDIKTIENPDDLEP
                     GSFRYKAILPGVAYMASLNASLEATILNRETFDEIFNIEKFRNMGYNSLVQFFDYEKL
                     KEGAEVTVRSREKGDVIKPLKSTGTKKLKEYMIDRKIPAEKRDLIPLLAKDNEIIWVV
                     GYRISDKFKVTENTKSILKVKYKLLK"
     gene            complement(13439..14773)
                     /gene="dnaB"
                     /locus_tag="PK733_09860"
     CDS             complement(13439..14773)
                     /gene="dnaB"
                     /locus_tag="PK733_09860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOJ10884.1"
                     /translation="MTELNLLGRIPPQNIEAEQSVLGSMLLDKEVIPVVIEILKKEDF
                     YRDDNAEIFDAIMDLFDKGEPVDIITTAEQLKLRGTLEKTGGLEYLTNIATGVHTTAN
                     AKYYANIIEEKSILRKLIRASSEITGMSYEAAEEVSIILDTAEKNIFDILQKRSSKGF
                     YHVKEILVDTFNRLEELYNNKDYITGVPSGFVDLDYKTSGFQNSDLILIAARPAMGKT
                     AFALNIAQYASVYKKIPVAIFNLEMSKEQLVNRLLCSEALIDSQKMRTGSLEDEDWDK
                     IARTLGPLSEAPIYIDDTPGSSVMEIRAKCRRLKLEKNLGLVVIDYLQLMQGRGRTEN
                     RQQEISEISRSLKILAKEINVPVITLSQLSRAPEARTDHRPILSDLRESGAIEQDADM
                     VMFLYRDDYYNPETEKKNIAEVIIAKHRNGSTGTVELIWLGQYTKFGNLKKE"
     gene            complement(14856..>15060)
                     /gene="rplI"
                     /locus_tag="PK733_09865"
     CDS             complement(14856..>15060)
                     /gene="rplI"
                     /locus_tag="PK733_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ10885.1"
                     /translation="KAGENGKLFGSITSMDIADRLNKSFKLDIDKKKIHLADAIKSLG
                     TTDVEIRLYPGVSTTVKVNVVQE"
BASE COUNT         4585 a         2986 c         2213 g         5276 t
ORIGIN      
        1 gaagctgttg ccacttcacc cttatatctt atgcttgcag taatttgggt tgtccctttt
       61 cctacaccaa cgatagcagg taccccgtcg ttatattcta cttttgctac actttcatca
      121 ctgctttgta tatcaatttt ttctgatatg gcaagatcta ctgctttacc attgctaaag
      181 agccctgata agcttatgtt agtggaagcg ccaattagaa gtcctgttgt tgatgtattt
      241 attgctgcac tttcaagatt aacctttggg caatcaactg ccggatcagc aaggtctgta
      301 gaaccataaa tctcccatcc atccagattt ccattattaa aaccttcttc gaatattata
      361 tcttctgcag tataaacttt aaaattatca aaatatgcac aaccatcgtg ataactagac
      421 cttatatcac ctatataacc tattgcattc attctcttgc caggagctat tctttgtcct
      481 gaatatgaac ctgcaatgcg tgcctgttta ccattaatat aaatttcaaa tgtgttgtca
      541 gctttgaaat gaagcataaa gttataccat gtatcagcag aaatcttaat gtctatctct
      601 tgccaattta atggtccatt ttctgctttt attaaattcc catgaaaagg gagcacccaa
      661 aacattacca tctatatctt ttatattacc atctacagtg acgctatatt tagtttcata
      721 ttcaaggaaa gccgaaatgg taaatgtaat tgttttgcct tctacagaaa tacttccgga
      781 gatttcattt ccctgaggat ctactacttt tattaatccg tcaatattat cagctgaaat
      841 attgttatta aaactaacaa caactggtac atcaatgccg ttactttgcc cttgaggact
      901 ataagaaact atcgcaagtc taaaaatatc atcctcgctg aattgatatg ctcctatgtc
      961 aactcctgta attctctcaa gtcctctata atcatgagta ggtgcatctg caggtgttcc
     1021 attgccaata caaatactat cgccttttaa tcggaaatca ttaatgctgc tatcaataaa
     1081 tgagatgttc ccaataacag cattttgctc cggagctgat ggaagcgcct taaaatctgt
     1141 aaaacttatt ccatcaggat gtgttggact tatgatccca atattaccag ctagattttc
     1201 attatatgcg ttgtaattga actgattcaa gccaacaata tcagcttcat tcatgcttag
     1261 cagatatagg cctcttgtaa ttatgttatt acagatccta ttgttttgcg gggtgcctaa
     1321 ggttggatgc gaaagaatgt agatatcacc ttttgcattg ttaaaaagag tattgttgta
     1381 tatggagttg ttactactat tgccaagtac atatatccca taatttgtgt tattatatac
     1441 aatattgttg tagacttttc catttttcgt atctcttact gttgctccgc tatcaccttg
     1501 tgctgctgaa aatgtgcttg aatataattt aaatgtatct actttaaata aaagcacatc
     1561 tccgcctgta gagtgtacat gtgaaatatt aatattactg ctgtctgaaa ctattaatgt
     1621 gttttttgat cttcctccgg aataagctat attaattccc ttgatagtaa tatcagagca
     1681 tttgctaact gaaaaatatt tttctgcttg atttattatg ctaacaatct cattatcata
     1741 gttttctata acaattccgc ttttaccgct taatacagct gcttcatcag tataagtacc
     1801 tccacgtatt acaagttcac tatttgaagg tattacagct aatgcatgtg taattgtcct
     1861 aaaggggctt tcgatacttc catcattatt gttatcacca ttaatagata cataaaattt
     1921 attttcatat tgaataacat tttgtgcact agtaacaatt tgcgataagt ttacaaaaag
     1981 aaaaataaat actaataaat atgtaagtat atttcttctg gtaaaattac ctagtttaaa
     2041 tttacccatt atcttcacct ctttaaattt aattttttaa attagaaata aaaatgtttt
     2101 gataattata attaaatgta attgatttat tctgattact tcataatagt taatctattt
     2161 acataattac gctgcatagt atgtcactca ataaagaaac ccttaaaaac aaggtttctt
     2221 tattgagtat taagtattat atgaacaaca attgtaagat tggaaaggtt tattgtttat
     2281 tgttttttaa ttgctttttg tctttgcata gtggtcgtta gcttctttaa gcatttggtc
     2341 tccaccattc tttttccact tttctacaaa ttcatcaaaa ctctcgattg gtttctttcc
     2401 tgttataata tttatataag tttcctgcat taaactatgc aaatcagtag caaatgttgc
     2461 gcctgaagga atgtacagtt tataccccat gtcttctatt ccatatctgt caaaaccttt
     2521 tttcaggaag ttatcaaaat atgcaccatt tatctttctg gtattattaa catcagcctg
     2581 ccagaagaac gctgcatatc ctacttcgcc tgtagctctt gccggatcgc tcttataagg
     2641 ctcagcaata ttgtacacat catatccggc ttctttagta actatatggt ggataccctc
     2701 ttcaccatat ctgttgaaaa tatagttttt ataatctgcc ataggccaat taaacatctg
     2761 aagtattctg cctagcttat ctggctctac gtttttagaa aatacataac ttgcgccggt
     2821 agacaaaggt agctttgttg ttccccttga tttcccatca ggtccaagag gtgggttccc
     2881 aaattcatat gttgcatcag gattaatttt tttcatttca ttgtaatttg caccaccgac
     2941 cagcccgttt ccttccttct gccagtgata agcactgccc atcgttgaaa ctccaatttt
     3001 actattaaca aaatctgtag aaactgccca ataaccgccc ttattttcac cagcaacaaa
     3061 ctcagggtca ataaggtcca tgccgtacca tctgctcaaa agtttcaggg cttctttagt
     3121 accgggctct atagcgctat ttacaatttt cccatccttt tcaagccaga aggtgttcca
     3181 ccacatatca atcatagcac catatgcgcc aaaaactggt ccaattatag ttcctgaaca
     3241 tccatatgta tctttgtttc catttttatc cggatcatca tttctgaact ttataagtgc
     3301 ttcttcccat tcagtgattg tatcaggaat cttttctatt cctacattcc taagccagtc
     3361 gtctctccat acaattgcat cacggatcat gtaattagct acaagataag gtataccata
     3421 gtttttgcct tcaaatgtac atacactcca ggattttgga tcatccctgc taattgcctt
     3481 taacatgtcc ggagcatatt tgttgattac ctctattggg agttcctgta tcaattctcc
     3541 atgctgatag cgtaaaaacc tatctgatga ttttgaagga aatacatcgg gaatgtctcc
     3601 agaggaaaga cgaatattca aaagatcgtc ccacttgctg ctttcaacaa aaaccggttt
     3661 caacttaaca ttaaacattt cattaaactt tctgccgata taactgtctt catttactgg
     3721 agcgtttgtc atagtgagat aggttatttc atataccttc ccatcaatag gctgatattt
     3781 gtccttctct tcgtttgcag ctttattgtc tttagcgctt tcacttttgg gtgttgtatt
     3841 atcttttgaa gaatctttga caacacagcc tgcaaataat gacatagcca ttaggacagc
     3901 tatcattaat gctgtaaatt tgataaattt ttgcttcatt gattttccct ccattattat
     3961 tttattattt tgttgtttta ctatattaac agacctgggt attcaaatac acagcctgcc
     4021 aattagtcaa ttacacagct ctgttacatt aatctgccga ctaaccttta actgacccta
     4081 ccataattga acctataaaa tatttctgca ggaacggata tgtaagaata ataggtattg
     4141 ttgtaaccat aattgtacat gctttaatta tctcctgagc tggattgtaa ttatccatag
     4201 ttcctgtaac agttctctct gacccccaat taagaacctg atataacact acctgaagta
     4261 caacaagttc ttttttcgta atatatagaa ggctgtcaaa ccatgcattc caatgaccta
     4321 cagcaaccca taaagctacc gttgcaagaa taggcttgca tattggaagt attatgcgaa
     4381 ataatattat tatgtcattt gctccatcta ttttagcgga ttcctccagg ctttcaggta
     4441 aagatatcat ataattccta atgagaataa gattgtatgt tgaaaccagt ccgggaagaa
     4501 ttagtaccca tcttgtatct atgagcctca ggctttttat caatagatat gtaggaatta
     4561 accctccccc aaagaacata ggtattaaaa taattgttgt ccagaatttt ctgtttggga
     4621 gatatttttt tgacaaggga tatgctccac atgatgtcac caatactgcc agaaaggttc
     4681 cggatattgt tcttagaaca gaatttttca agccagttaa tattgcatcg gtcctgaaca
     4741 gttgtttata gtattcaaga gtaaaacctt tgggtaaaag gtttaaacca tactgggcag
     4801 aagaagcccc tttgctcacg gatatattaa acacgtatag aaaaggatat atacataaca
     4861 atgagaatat aatcaaacat attgcattag ctacatcaaa taccatttct cctgaacttc
     4921 tttttactgt tgacataaaa cctgacctcc aagaaataat ctatatttat accaaagtat
     4981 tttgtaataa ttgttaaaat ctatacactc taaataatcc tgtattcatt atcgcttatc
     5041 ttttttaata tatagtttgt caggaaaata agagcaatcg ctattacatt tttgaaaagt
     5101 ccaactgctg cgccataact atattgcatt aataccatac ccattctata ggtgtatgta
     5161 ctaataacat cacttatctg cataacacca gggttcataa ggttgaaaat ctgatcaaaa
     5221 tcatcgtgta taatattacc tatactaagt ataaaaacaa aggttataat tggcacaagg
     5281 gatggaagtg taatcttcac cgctttctgg aatctgcttg ctccatctac tgttgcagct
     5341 tcgtatatct caggatttac gtttgccata ccagcgaggt atattatagt cccccaaccc
     5401 acttccttcc atatagaagt tatcacgagc acaaatctaa accagttggg atctacaaca
     5461 aaatatattg gcttaatatt caaagcattc attacatagc ctacgggtcc aattgaaggt
     5521 gataaaaact gctttactat acctgacagt attacccaag ataaaaaatg tggaagatag
     5581 cttatagttt gtactgacct tttaaaccag cttaccctta tttcatttaa aagtattgca
     5641 agaataattg gtgcaggaaa tccaaataga aactttaatc cgcttattat tagtgtattt
     5701 ttaaatactc ttaaaaagtt attaccttga aatatgagct taaaatgctc aaatccaatc
     5761 cattcactac cccatattcc cctggaaaat ttataatctt taaaagcaat ttgaatacca
     5821 tacattggtg cataatgaaa tataccaaaa tataaaaggg taggtaaaag caatatgaac
     5881 aaatatttat tatctttgta cttttttaaa ccattgataa ctttatttaa aatcacaatt
     5941 ccggagtttt gctttaaaac tccatgttcc ttggttttat ttagattcaa cgttttcgtt
     6001 tccatataat atgatgcctc cctaaagtct tcaaaacaaa aacttcttta agttgtcttg
     6061 tttgtgtttg gttgttcttg caattgctat tctaacttga gttaatataa aacacaatta
     6121 tatagatttc atattcttaa gaaatgtatt tataatgttt taatattctt ataatatttt
     6181 aatatggtta tatttaccaa tacagttaaa ttggcttaaa tagcatatta cagcactttt
     6241 actatgtata ttgattaata agcgaatatt ttgaataatc attccaggca tgttataata
     6301 aaaacaatta aggaaataaa acctatataa tttacgattt gaaagagcta atgaagaatt
     6361 tgcaaatcgt aagttacatc aaggtaacag aagcaggttt aaatgtttta aacctgttct
     6421 atatagcagt tttatatttt tgtatttatt gaaacggtgt gatgcaatcc gattggctgg
     6481 ttgcatttat gttgtaatgg tgtcatacaa atatataaca acctgtaaaa tctaacaagg
     6541 tgaataacgc agaaatttat tgttaagata taaggagtat gcctatgtat tcaggaatta
     6601 aattacctgt acgtgctcgg aatacagttt taataaggtt ctttctctac tttttaatgg
     6661 taattcttct aacattaact acaataagct actttttgta taattacttt tcatccttat
     6721 ttaaagatga agttttaagt ctgaataata gaatacttag ccaagtttct gatttctcag
     6781 atgcttttat attaaaaaat atgaatgagc tagctgcagg tatttctatg ccatacccac
     6841 atgaaacttt tatttcacaa tttttttcag attctgatga ggattactgg aaagttattc
     6901 ttactacccg taataagctt gatagcctca tttttcagaa cagggatatt gttgattcta
     6961 tctatcttta ctcaaaagat aagaaattgg cattatctca aaaggtaatg aaatatattg
     7021 atgaaaagga agccagaaat ctggatgaat tttcatggat aaatcaggct tccatgtcaa
     7081 attcaagtgt tatatggcta aatactcgaa ttgctaagat atacggttca caatctgcag
     7141 ataatggaaa tataattacc cttatctgta cttatcctgt ttcatctcgt ccgcaagatg
     7201 caaaaggata tgtagctgtt aacattaggg aagaagcctt aaataaccac cttgtgaaat
     7261 ataactcaac gaagcttggg catttgctga tcgtcgataa aaatggttca attatatcac
     7321 acagtgataa gtcaaaacta tataatgata tatcaaagga acgttttgtg aaggaaatca
     7381 tgttgtcaga gaaacctaaa agctttcaat ccaaatttga aaatactgac tatatcatat
     7441 cattcaaaca atcagcatat aacaactggt attatgtagc tatggttcct actgaactcc
     7501 tttatcagaa agattatcac ataaaaagaa aaatttttgc tgcatcaatt acaattttct
     7561 ttattgtact tctggtttca aatgttttca cctacaaaat gtataaacct ttaaagaaat
     7621 tacttgataa atttgcatct acaataaata gcgaaaaaaa gggtaaattg gatgaatatc
     7681 ttatgctgga aaatgcgttt acagatatgt ctttaaagat agataatctg gaggatacac
     7741 tttgcaagaa tgtagatatg atagtgcata actatttgat tgatctcctt aataaaccct
     7801 ctattgataa tcctaacatt ggaacaactc ttaactcctt gggtgtagcg ttttctgaga
     7861 agtattttac tgtaataata tttaacatat caaaagcagt agtcgatttg cataaacaga
     7921 ctgatattca actttgcaaa tataagatat tcgaatatat aaaaatggtt gattctacca
     7981 acacagataa ttcatatata gatatggata aaggcacaga tacaaataca aaaccaatct
     8041 gccgctatta tccaatagat actgacagcc ggactatttc tgttattgtt aactcaaata
     8101 ttaatgatct ggcgcttgtt cgtaatttta tcactcatac acagtttcac tgctataata
     8161 actttggttt ttacctgact gtgggagttg gaaaatattt tgacaatctt ctttccgtac
     8221 gccaatcaaa tattgatgca aaaattgcaa tactttataa gttccttcac ccgggccaaa
     8281 atgaatttta ctatgaggag atcagccgca aacctgcaaa tcctgaggtt ctaaatcagg
     8341 attacatgga aaaactcttt aagtcgctaa agctttgcaa cataaccgaa gttaacaata
     8401 tacttgatac ccgtatatca acaatcagca gcaacaattt taactatcaa tatataaaga
     8461 aggaaatatc tcagttaaga aaactttata ttgagtgttt tgaagaaatg agtgtaaaga
     8521 tcgacagtaa ctctgaaatt aaagacatat ttttctatcc tggagatgtc tttgaattct
     8581 ttgatgcttg taaagttatt tcaaataagt ttattgacta catgaaagag aaacggacca
     8641 gcaaaacctc agaattaatt gaatccgtgg aactgtatgt tttgaataat attaacggaa
     8701 acctttccct taatctggtt gctgattcat tacgtattag tcctcagcat ttaagcaaaa
     8761 tttttaaaga agaaaagggt attaatttta ttgattatgt aactgacaaa aaaatagata
     8821 aggcaaagaa ccttctctca acaacaaaca tgagtttgga tgaaatctca gtttgtgtag
     8881 gctatagcca tgctacctat ttttcaagga agtttaagga gtctacaggg tacacccctg
     8941 gtgaatacag gcagcaagag gtgaaaaagc agttgaagtg agtattgagg aaccgtcctc
     9001 aatgactcac ttcaaagtaa cttgctgatt ttattcactt gcctacttat tcactacatt
     9061 tactatattt actgcaatac tatatttact gtctgccact tattaccgat taccaattag
     9121 ctattgtcgc ttaccaattg tcaacagcct tacgtattgg caaataactc ctcaaattcc
     9181 tgtccttcga gcttctcctt ctccataaga ctgtttgcta tggaatggag cttgctgata
     9241 ttttctttta aaatatcaat tatcttaacg taagcagtat ctataatctt tttaacttca
     9301 ttgtctattt tggcagctac ttgctcacta tagtttctgg tgtgagcaaa atcccttccc
     9361 agaaatactt catctgtttc gttgccaaat ataaggtttc caaggttttc actcattccg
     9421 tatttggtta tcatgtttct ggcaacacca tttgcttgct taaggtcatt ataagcacct
     9481 gtgcttactt ccccaagaac gatttcctct gcagctcttc caccaagagc aattgaaatc
     9541 tgttccagca actgacccct ggtaagatag gtcctatctt catcaggttt aaaagctgta
     9601 tatccgcctg ctcttcctga aggtattatg gaaatcctgt cgaccttgtc ggtagatgat
     9661 attactttga cagcaatagc atgaccagct tcatggtatg cagtgagttt cttttctttc
     9721 tcactcatta ccctgctttt cttttcaggc cccataataa ctttgaaagt ggcttcttta
     9781 atttcttcca tttccacttt tcttttattt tttcttgctg caagtagagc cgcctcattt
     9841 aaaagatttt ccaggtcggc acccgtaaac ccgggtgtgc tctttgcaag ttcatcaagc
     9901 tttacatcct gagaaagagg tttgcctctt gaatgaactt tcaatatttc ttctcttccc
     9961 ttaatatcag gtaatcctac aaatattcgt ctgtcaaatc ttcccggtct caataaggca
    10021 gggtcaagaa tatcaggcct gtttgttgct gctaatacga taacaccctc attttctcca
    10081 aagccatcca tctcaaccag cagctgattc agagtttgct ccctttcatc atgtccgcca
    10141 cccaagccgg ctcctctgtg tcttcccact gcgtcaattt catcaataaa tactatacat
    10201 ggggcgtttt ttttggcttg atcaaaaaga tctcttactc tggatgcacc tacaccaaca
    10261 aacatctcca cgaaatctga accactgatg ctgaaaaacg gcactccggc ttctcctgat
    10321 acagcccttg caagaagagt tttgcctgta cccggaggac caacaaggag cactccttta
    10381 ggtattctgg caccaagctc aataaacttt cttggttgct tgaggaactc aactatttcc
    10441 tccaactcct ctttttcttc atcagcacca gccacatcat tgaaagttac tttccttttc
    10501 tcatcagtac tcatttttgc cctgctttta ccgaatgaca taacccggtt tccaccaccg
    10561 ccctgcgact gctgtaggaa aaatacccaa aacagtacaa agataacaat aagccctaca
    10621 gtgggcaata tcgctatcca ccatggagcc gtctgcggaa aagcggtttt aaatcttatt
    10681 tgctgggcct taataggact ggaaacttca ttcataaatg aagataaatc cggtatatat
    10741 atgacatacc gattaactct gtcagtaata tatggctttt tcaaaacaac agttgctttt
    10801 tgattgtcaa gaagaatttc acttacatta ttattttgga tttcccttat gagatctgag
    10861 taatccatat ctacaggatt atcagttccc tgtgataaag ccaatataaa taatattatt
    10921 acaaatagta ctatatagaa gctcaatccc tttaaatatt tcaaatatct ccctccctgc
    10981 ctgcagtcca atcattgggt ttggcagaat tcaatgttct ttatttacca ttaatgcagc
    11041 atttttcccg ttattttatc ttagcatata taaatttaaa atacaataaa atatatcata
    11101 aaaaaggaac cctttttttt aatacatttt aataatgcct taatcatgtc tgtaaacatt
    11161 cggatcgagt atgcataaat ccggtaaatt tctatatctc tcagcaaagt caagtccata
    11221 accgataaca aactcattag ggatttctat tcccttataa tcaacattga tatccaccaa
    11281 tctccttgaa ggtttatcaa atatggaaca gattttaaca cttttaggac ctcttgtgct
    11341 gaaaaggttc tttaaatggt ttaatgtaag ccctgtatcc acaatatcct caacaataag
    11401 aacatgcttg tcggaaatac ttacatccat atccttaata agccgaacta caccagatga
    11461 ttttgtacca tcagcatagc tggaaacagc tataaaatcc atatcaacag gaatagtaat
    11521 ttccctgatc aaatctgcaa gaaaaataaa cgctcctttt aatactccaa tcaggaccaa
    11581 ttccttacct tcataatctt tagaaattat ctgtccgagt tctcttacct tttcagctat
    11641 tttctctctc gaaaccaata cttcctttat ttcacttcta ttagtaacat tattaacagc
    11701 aacattagca gcattattag tacttttatt tgtacccatg ttttcacccc atcatttatt
    11761 gcagaaaata ttaaattaac accatatcaa attcaattta tcattttagt aatttgtatt
    11821 taacttttaa tatactttta gtattttcag ttactttaaa tttatcactg attctgtatc
    11881 cgacaaccca gattatttca ttatctttcg ccaataatgg tattaagtcc ctcttttctg
    11941 caggaatttt tctgtctatc atatattcct taagcttttt agtgccggtt gatttaagag
    12001 gttttatcac atcacctttt tcacggcttc ttacagttac ttcagcacct tctttcagtt
    12061 tttcgtaatc aaaaaattgt accagtgaat tatatcccat atttctgaat ttctcaatat
    12121 taaatatttc atcaaatgtt tctctattta atatcgttgc ttccagactt gcattaagag
    12181 atgccatata ggcaactcca ggcaatatgg ctttgtatct gaaacttccc ggctccagat
    12241 cgtctggatt ttctattgtc ttaatatcgg ggttgtttaa ctcattctta taattgttat
    12301 ttacatatat ttttaaaata tcataggact ttgcagctct aataccaaag ggaaggtgta
    12361 tttccgctcc tgttcgccca tccaggcaca gttcaagtat tttgtctata tgtatacttt
    12421 caatacactt taaatttcct ctgacactat ataccgcttt tcttattact cttctcctaa
    12481 ttgcattatg taaacttctt atgcgcctga tatccaaatt caccatattc ttaacattct
    12541 taatgtaatt accttcatca ccgttcttat catacagcag gcacctttta tattcctcat
    12601 ctgcagttaa atccatataa ctgtagtcat ctcttattag gtcagccatc ctgttaatgc
    12661 tttctacaat attaatataa aagttatgag atataaacgg tataagctct aacctgactt
    12721 tgtttcttgt ataaatactt tgaaggttcg tactgtcagt ccgggttttc aggttgtgct
    12781 ccctgcaata ctcctcaatt tcttttcttt caatatctaa caaaggtctg attattctgt
    12841 ttcttttata gtccattcct ctcagcccgt caagtccggt accccttatt atgttcataa
    12901 taatagtctc agcctgatca tttctgttat gagctacagc aaccttgcag ttgccgattt
    12961 tatctgccag agtattaaac tgtccatatc ttgcttctct tgcagcttcc tctattgata
    13021 tattttgttt ttctgcaatg tcccctacgt tcagggatac ggtaaaaagc tgaatcccca
    13081 ggctctcaca caactccttt acatatcttt catccatatc cgattcctca ccacgaagca
    13141 tatgattaac atgaacagca tatagtttta tcctcatttc atgagaaaaa gaatatagta
    13201 tgtgcagcag acatacagaa tccggccctc cggaaacccc gacaaggata ttatccccag
    13261 gttctataag gtcatgttct cttattgtat caagcactct atccggtact ttatccggta
    13321 ctttacccgg tattttgtca ggtactttga ccggtacttt gcctagtaat ttgtccggta
    13381 atttattctg gcctttattt agcattttat tctggtattt actccttttt aatggtcttt
    13441 actccttttt taaattcccg aattttgtat attgcccaag ccagattaat tctacggtac
    13501 ctgttgaacc gtttctgtgt tttgctatta ttacctcggc aatattcttt ttctcagtct
    13561 cgggattata gtaatcatct ctatataaaa acataaccat atccgcatcc tgttcaatag
    13621 ctcctgattc tcttaaatca ctgagaatag gcctgtggtc ggtcctggct tccggtgctc
    13681 tgcttagctg tgatagagtt attacaggaa catttatttc ttttgccaat atttttagtg
    13741 aacgggatat ttccgatatt tcctgctgcc tgttttcagt tcttcctcgt ccctgcatca
    13801 actgcaaata gtcaattacc accagtccaa gatttttctc aagtttcagg cgcctgcatt
    13861 tagctctaat ctccattaca gaacttcccg gagtatcatc aatataaatc ggggcttctg
    13921 atagcggacc taaagtacga gcaattttgt cccagtcttc atcttccaga ctccccgtcc
    13981 tcattttttg gctatcgatc aaagcttcgc tgcataacag tctgtttaca agctgctcct
    14041 tggacatttc aagattgaaa atggctacag gtatcttttt gtaaactgaa gcatattgag
    14101 caatgttaag agcaaaagca gttttaccca tagcaggtct ggcagcaatc aatattaaat
    14161 cagaattctg aaagcctgat gttttataat ccaggtccac aaaccccgat ggcacaccag
    14221 ttatatagtc tttattattg tagagttctt caaggcgatt aaaggtatca acaagtattt
    14281 ccttaacatg ataaaaacct tttgaactgc gtttttgaag gatatcaaaa atattctttt
    14341 cagctgtatc aagaataata gaaacttctt ctgcagcttc atagctcata cctgtaattt
    14401 cagaagaagc tcttataagt tttctcaata ttgatttttc ttcaataata tttgcataat
    14461 attttgcatt tgcagttgta tgtacgcctg ttgctatatt ggtaaggtac tccagtccgc
    14521 ccgttttttc caatgtaccc cttaacttaa gctgctctgc tgttgttata atatcaacag
    14581 gttcaccttt atcaaaaagg tccataattg catcaaatat ttctgcattg tcatccctgt
    14641 agaaatcttc ctttttcaga atttcaataa caacaggtat aacctccttg tccaaaagca
    14701 tcgaaccaag gacagactgc tctgcttcaa tattttgtgg tggtattctt ccaagtaaat
    14761 tcaattctgt catttcttat aatcctcaat ttaatatttc tgcgaaatat caatgccatc
    14821 actcccgcag aatggctatt atgtcatcaa tgttatcatt cctgcacaac attaactttt
    14881 acagtagtgg aaaccccggg gtaaagcctt atctccacat cggttgttcc aagacttttt
    14941 atagcatcag caagatggat tttcttttta tcaatatcaa gtttaaagct tttattcaat
    15001 ctatctgcaa tatccattga tgtaatagat ccaaaaagtt tcccgttttc tcctgccttc
//
LOCUS       DAOSDD010000096        14849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1607_length_14849_cov_5.054076, whole genome shotgun sequence.
ACCESSION   DAOSDD010000096 DAOSDD010000000
VERSION     DAOSDD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_14849_cov_5.054076"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..296
                     /locus_tag="PK733_09870"
                     /pseudo
     CDS             153..296
                     /locus_tag="PK733_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
     gene            complement(364..1578)
                     /locus_tag="PK733_09875"
     CDS             complement(364..1578)
                     /locus_tag="PK733_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ10886.1"
                     /translation="MGKLQQVIIAVNNFSLGILLPVLNLVLMERGATLQTLPLLVGIY
                     SVTVLCLELPSGVCADIYGRKMIFLMSTVFMLMSFSLLIMANSMGWLIFAMIFFGMGR
                     AFSSGSLDALFIEQAIDLHGECCLAGVTARISILESVGLAVGAIAGGIIANATGTYLT
                     NIVVRLVLTAILFILCLFFVKEQPLHGKSHHTTLMEHVKQGKQVLTSVHELGFVFIGV
                     FFLGFLFFTVETYWQPAFMQMSNTQESTWMLGFITFIGFFSVAFGNTIVKKILDKYIN
                     KWWNVYNICRIVFAGCIVIFAIQKNSISFVVCYAFIYMALGASNLAESALINKFTQNH
                     MRASMLSLNSLVVQIGSLCASLFSSIMILKLQFAGLWIVAGVLLGGYAVISIAVINKY
                     NQKNRNNTTVIP"
     gene            complement(1571..2146)
                     /locus_tag="PK733_09880"
     CDS             complement(1571..2146)
                     /locus_tag="PK733_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10887.1"
                     /translation="MKKIKLTTREELNIYMSPVRQQLLRELSISKDPMTPKALSDKLK
                     ISASSVQHHIKKLMSLGLIELDHIALINGIRASFYRRAQVLVQIGLDRSDDLSLQRFA
                     LMQDSIAQVYDGFCKQMKKRTSLQGNNDLKSMWKWGDILTGVAHLNEQESDVLMKLIV
                     SFLEQHSRPITGSTPWEYAIIAYKAGDICDG"
     gene            2327..2500
                     /locus_tag="PK733_09885"
                     /pseudo
     CDS             2327..2500
                     /locus_tag="PK733_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966811.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
     gene            complement(2624..3103)
                     /locus_tag="PK733_09890"
     CDS             complement(2624..3103)
                     /locus_tag="PK733_09890"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HOJ10888.1"
                     /translation="MNIYVFDSSALMHLFDYYYESRFPTLWRRFYQSVESGQVVSVRE
                     VRNEINGHHNKERRINQWAKQNTELFTIPTLEEMQFVQEIFKVEHFQVIISRKNLLSG
                     KPVADPFVIAKAKIIDGTVVTNEANKPHGAKIPNICEHFNVKCMDLEKFMEVEDWRF"
     gene            complement(3100..4263)
                     /locus_tag="PK733_09895"
     CDS             complement(3100..4263)
                     /locus_tag="PK733_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ10889.1"
                     /translation="MVKAPVNPDVMRWARESANLTVDEVMVKLKKSFEVIEAWEKGLA
                     SPSYAQLEKLAYEVYKRPIAVFFFPHPPKEDDIKKSFRTITEAEFNRLPSTLIRQIRK
                     ARVKQENLYELCNGVNPSSKQLPNDLKDIVGHDLKEIATYIREYLDISLSKQKSWDTI
                     ETALETWREAFEDSGVFVFKEAFRNDDFSGFCLYDKVFPLIIINNSMAKTRQIFTLFH
                     ELGHLLLEISGIDKLNDDFVNILSEQDRDIEILCNRLAAEILVPSDDFDLSVRGMRVD
                     EASIAKLAVIYKVSREVILRKFLDRGAVSESTYKELTTKWIEEAKKKKLGSKGGGDYY
                     NTQIAYLGDKYLEIAFNAYYRKTINDMQLADYLNMKIDNLNSLELSLHRRWSR"
     gene            4496..4672
                     /locus_tag="PK733_09900"
                     /pseudo
     CDS             4496..4672
                     /locus_tag="PK733_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
     gene            complement(4830..4985)
                     /locus_tag="PK733_09905"
     CDS             complement(4830..4985)
                     /locus_tag="PK733_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10890.1"
                     /translation="MIIIGIIVTIVIGVIGTSIGYGMNAAELGPILSIATMGGFIMAY
                     IKKNNIK"
     gene            5657..6352
                     /locus_tag="PK733_09910"
     CDS             5657..6352
                     /locus_tag="PK733_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10891.1"
                     /translation="MRETTLRQIVYSAVSIILKQDGLTRTELHELADFILDNSSLNLS
                     AEKICLAEGNYFGVGEYNKDRGFALPRFITFELTRPSAFDIMNNNKSKTWRLRSHETF
                     EELENYLTYSRQDQPAVFIDGTHTEYNLMGTETWGYEVQWIGCDAVYIATYVLNNKEE
                     KELINSKLQLQRKLIELVEQGAEEIQAYNVVQGENMPFKVQGVLYNSNQLKDNRKGLV
                     WVTKNEDECEDPF"
     gene            complement(6425..7177)
                     /locus_tag="PK733_09915"
     CDS             complement(6425..7177)
                     /locus_tag="PK733_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10892.1"
                     /translation="MGKYNKTAISRKLEGITEGEFEHLGIPELTQSEIESITGVPKQQ
                     INSYINGTIPRMDTVVKLSEGLGVSVDFLVKDEVVEPKADTQAIFQETGLSSDAFKIL
                     KTFKLKKEINLPLVINALELLIKNAGNWYNDPLTAIGKFLQLSADDTIYLLNEKRFDD
                     FEDILNSSNSVEDIRNEYEKLIHGSPETLDSNGQPPAIQTISRNQLLLQNIQNELSSL
                     KRTLWSDIEEVTANNLDNDDVIPVDWSSSAIL"
     gene            7515..7643
                     /locus_tag="PK733_09920"
     CDS             7515..7643
                     /locus_tag="PK733_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10893.1"
                     /translation="MGAMKNEGKQSKAVNNVFGMTGFINGAVPFGYKVNKNKELGR"
     gene            7671..8063
                     /locus_tag="PK733_09925"
     CDS             7671..8063
                     /locus_tag="PK733_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10894.1"
                     /translation="MKNITFIVTEATARDMGYSGNADLVRVNLQVDLSRLSYKARKLA
                     ESVWTLERADDLSKVYVTGPTQRELYIKDGRLDELKRYECIYGSEYFDKPTVIVWGWD
                     CLKKHETPEEYFERHVKMIGEAGGFPFQ"
     gene            8145..8489
                     /locus_tag="PK733_09930"
     CDS             8145..8489
                     /locus_tag="PK733_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10895.1"
                     /translation="MANTMKTKIRLPISTSSLEAIKKEFQHISVEKDGVYAITRGFVL
                     HNLLKALSKENNEEIIAEHSSSIDWYSTLYIERYKDGNSETIKTKTSEIIMYEITDPE
                     EIEEILNEQESC"
     gene            8470..8712
                     /locus_tag="PK733_09935"
     CDS             8470..8712
                     /locus_tag="PK733_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10896.1"
                     /translation="MSRRVVNKNLIPALLAGAVGQTPKQSATDLKMLHDYNFAFNIIL
                     IAADDIVNLGDKIDMNWCIQTAYELDNGPKKYFWEV"
     gene            8714..8971
                     /locus_tag="PK733_09940"
     CDS             8714..8971
                     /locus_tag="PK733_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10897.1"
                     /translation="MYSNVRGKLVESKVITIAPKGLKEYIVNKLDSGLEINETQIWKL
                     RARTFEKQRLSMVKFLRMRIFLPLQDSLSFNYGRNTTSYQI"
     gene            9110..9646
                     /locus_tag="PK733_09945"
     CDS             9110..9646
                     /locus_tag="PK733_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ10898.1"
                     /translation="MLITYKANNDEIRFADPDINELSKEQVIIELGKSKFRLNKLQQQ
                     IDREKALHEKLLKRRLEFYKYEKAEELKANNNMNLSSKKSQIELYYSQGKGYDEIALL
                     TEASPAYIYNVVCSYRKKNTVPIKNPEKTEIEEAIIMYSRVNKNIKEIAKNSNVSRQY
                     VYKVLKKHNIPYPSIKRK"
     gene            10243..10464
                     /locus_tag="PK733_09950"
     CDS             10243..10464
                     /locus_tag="PK733_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10899.1"
                     /translation="MGFSYDKLWKLLIDKKMKKVDLGNKAAIGPNTMARLSKGQPVQM
                     NVLGKICKELQCNIGDIVDYIDEAGGDSK"
     gene            10461..12989
                     /locus_tag="PK733_09955"
     CDS             10461..12989
                     /locus_tag="PK733_09955"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOJ10900.1"
                     /translation="MSAKEAKARIKINKLLEEAGWRFFDEDGKPANIQVEPNVKISKK
                     QVDDMGENFDKVKNGFIDFLLLDDKGHPFIVLEAKSEDKDPLVGKEQAREYAKSLFIK
                     YVILSNGNQHYFWNIYKGNPQIITSFPNCESIRENKAFNADPSRLYNEDVKEDYIAVV
                     KNPRYAEAPEYQNPETRKKFLKDSGLRFLRPYQLNAVKALQKSVKKGNQRFLFEMATG
                     TGKTLTSAAVIRLFLRSGNAHRILFLVDRIELENQAKKNFQNYLSPDYHTVVFKESTE
                     DWRKAEVVVSTVQTLMFNNKYKKYFKPTDFSLLIVDESHRSINGNSRAVFEYFLGYKL
                     GLTATPKDYIKNVDIDKLKSTDPRAWEKRQLLDSYKTFGYESGDPTFRYSLLDGVKDG
                     YLINPTVVDARTDITTQLLADEGYAVINQTEDNDDEEEVYYAKDFERKFFSEETNKIF
                     VKTFMENAKRDPLSGEIGKSIIFCVSRKHAAKITQILNEFADKLFPDKYYSDFAMQIT
                     SDVMNAQTYTINFQNNNLSGTTKFLENYKSSKTRVCVTVGMMTTGYDCEDLLNIGLLR
                     PIFSPTDFIQIKGRGTRKFEFNYKDKIAGEIQEHKFEKDTFKLFDFFGNCEYFEEKFN
                     YDEVINLPKPQSKGADTKTPPVDGTMYENFDPDPLKFIKETPIGYEGMKIDRMFFDKF
                     ESTVKEDETAKEQYEQGNYAAVQHYIEEQIMNKPSEYYTWDKLKRSIGIDRRVTVKEI
                     LDKIFGAIPAFKSKRELIEDEFESYLLTRPIQADKYTEIKRFFETYIMDKDVRRIIED
                     EKYQLLGTEVSTYTMSDLKRLGVDNMRQVVDYINDNVNVTRFVA"
     gene            13000..>14849
                     /locus_tag="PK733_09960"
     CDS             13000..>14849
                     /locus_tag="PK733_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ10901.1"
                     /translation="MLNFDVKRKINTLRDILVGKVPDPKAQVEQITIALIYKFMDDMD
                     IEGMEFGGSREFFKEDYEKYAWTNIMQPENSGQQRANLYAEGIDKMSMNPHLPQLFRD
                     IFRGAYVPYRDAETLNMFLKEINDFSYDHSEELGNAFEYLLSIMGSQGDAGQFRTPRH
                     IIDMMVEIVDPQKTDSILDPACGTSGFLISSYKHILEKNKDNDGNSTLSADERKRMTE
                     NFAGYDISPDMVRLSRVNMYLHKFAKPQISEYDTLSSLDKWDETYDVILANPPFMTPK
                     GGIIPHNRYRIPAKRAEVLFVDYIAEHLNPTGKAGIIVPEGIVFQSANAYKSLRKYLV
                     EDDLLYAVVSLPAGVFNPYSGVKTSILLMDRTLAKQKNEILFVKINNDGFDLGAQRRE
                     VKGSDIPEVINIIKDYQNGIDISDNTLVTIASKKDIAVQDYILVGERYKEAVVVNSDY
                     PLVELGEICLVERGTSITSKELIDGDIPVIAGGQQPAYFHNISNRTGKVITVSGSGAY
                     AGFINYFEEPIFASDCSTIQSNNSNVNLTYVYHTIKAQQDRFYELQSGMGQPHVYARD
                     IKPFKIPLPPLHIQEEIVKEIEGYQKIIDGARQVVENYKPTIKIDPSWEMV"
BASE COUNT         5227 a         2418 c         2764 g         4440 t
ORIGIN      
        1 caaaatttct tacgtcagtt gaaacttaat atagtaccga gaggccctag gataacggag
       61 gcactattac tctggacatt gaatatgtgt aatgatcata aaaataatag tatttaaacc
      121 tgtaatactg ggtatgatat atcttataaa gaatacccaa tagattagcc aatgagaagt
      181 ctccgtactt acttcagcat gctcacaacc ctgttgattg gttcccttgg agtgacgagg
      241 cgtttcagaa agcgaaagca gaagataaac ctgttttttt atctattggg tatagctgaa
      301 tttgctgtta atttcgcact tgtcactgat ggcaaaccgg ttactagaac caaatattat
      361 gggttacggg atgacggtag tattattcct gttcttctga ttgtatttat ttatgacagc
      421 aattgaaata acagcgtacc cgcctagaag cacacctgca acaatccata atcctgcaaa
      481 ttgtaacttt aatatcatga tgctactaaa taaggaagcg catagtgatc cgatttgcac
      541 caccagagaa ttcagtgaaa gcatgcttgc gcgcatgtga ttttgtgtaa atttgttaat
      601 taaagcactt tcagccaaat tgctggctcc taaggccata tatataaagg cataacagac
      661 aacaaagctt attgagtttt tttgaattgc aaaaataaca atacagccgg caaatacaat
      721 acggcaaatg ttatagacat tccaccattt gttaatgtac ttatctaata tttttttaac
      781 aatagtattt ccaaaggcta ctgaaaaaaa gcctataaag gttataaagc ccagcatcca
      841 tgtgctttcc tgagtatttg acatttgcat aaaagcaggc tgccaatagg tctctacggt
      901 gaaaaacaaa aaacctaaaa agaaaacccc aataaataca aaacctaact cgtgtacgga
      961 agttaaaacc tgtttgccct gctttacatg ctccattaat gtggtgtgat ggcttttacc
     1021 atgtaatggt tgctctttta caaaaaacag gcacaagata aaaagtattg cagtaagtac
     1081 taagcgcaca actatatttg ttaaataagt gccggtagca ttagctataa ttcctccagc
     1141 aatagcgccg accgccaggc cgacactttc caggatggat atacgggcgg ttactccggc
     1201 aaggcagcat tctccatgca ggtcaatggc ctgttctata aataatgcat ccaaacttcc
     1261 tgaagaaaaa gcccgaccca tgccgaaaaa tatcattgca aatatcagcc aacccatact
     1321 gtttgccata attaacagcg aaaaggacat taacatgaac acagtagaca tcaggaatat
     1381 cattttgcgt ccatatatat ccgcacatac accgcttggt aactccaggc acagaacagt
     1441 cactgaatat attccaacaa gcaaagggag agtctgcagg gttgcacctc tctccattaa
     1501 aaccagatta agaacaggta acaatatacc caaagaaaaa ttgttaacag caataatcac
     1561 ttgttgcagc ttacccatca catatatccc ccgctttata tgcaataatt gcatattccc
     1621 atggagtgct gccggttatt ggcctggaat gctgctctag aaatgataca ataagtttca
     1681 ttaatacatc agattcctgc tcgtttaaat gagcaacacc ggtcagaata tctccccatt
     1741 tccacatact ttttaagtcg ttattcccct gcaggctcgt ccgctttttc atctgtttgc
     1801 aaaaaccatc atacacctgt gcaattgaat cctgcatcag tgcaaatctc tgcaaagaca
     1861 gatcatctga tctatccagg cctatctgta ccagtacttg agcccgtcta taaaaactgg
     1921 ctcttatacc gtttattaat gcaatatgat ctaactctat aagtccgagg gacatgagct
     1981 tctttatatg gtgctgtaca cttgaagcag atatcttgag tttatcagaa agcgcctttg
     2041 gagtcattgg atcttttgaa atgcttaatt cgcgtaacag ctgctgccgt acaggactca
     2101 tatagatatt tagctcttcc ctggttgtaa gtttaatctt tttcataagg aaacacctcc
     2161 atacgttaca tacattataa cattacgaac cgtgcaacgt caagcatata gtataccaca
     2221 tgaggctgtt tctcgtttgt tttttttata gcaaatcaat aaatttattg tttttatcta
     2281 attagggtaa taataaacaa aacatttttt taggagttga aaataaatga atgaacataa
     2341 atatacaaac cgcctttccc aggaaaagtc tccctacttg ctctaacacg cccataattc
     2401 tgttgattgg ttttcttggt ctgaaaaagc ctttacaaaa gcaatcatag aggacaaacc
     2461 aatattcctc agcatcggct attctacatg tcactggtgt cgtgcccgtt attaacagca
     2521 ataaagagcc ttctcacgcc tgcatttgaa tgcctttctc accattcaat attttctata
     2581 ttgtgtttga taaatatgca tcagattgaa ttagatatgt acgttagaat ctccaatctt
     2641 caacttccat gaacttctca agatccatac atttcacatt gaagtgttca caaatatttg
     2701 gaattttagc tccgtgtggc ttgttagctt cattagttac tacagttccg tcaattatct
     2761 ttgcctttgc tataacgaag gggtcagcaa caggtttacc acttagtaga ttctttcgag
     2821 atatgataac ctggaagtgt tcaaccttaa atatttcttg aacaaattgc atttcctcca
     2881 aggtcggtat tgtgaataat tcggtgttct gcttcgccca ttggttgatt cttctctcct
     2941 tgttatgatg tccattaatc tcattcctta cttctcgaac cgatacaact tgtccgcttt
     3001 caacggattg atagaacctc ctccaaagag taggaaatcg tgattcataa taataatcaa
     3061 acaagtgcat caatgcactt gaatcaaata cgtagatatt catcttgacc acctcctatg
     3121 aagtgatagc tctaatgaat tcaggttatc tatcttcata ttcaggtagt cagctaactg
     3181 catatcattt attgttttcc tataataagc attgaaggca atttcaaggt atttgtctcc
     3241 aagataggcg atttgagtat tgtagtaatc tcctccgccc ttactcccaa gtttcttctt
     3301 ctttgcttcc tcaatccatt tggtagtcag ttccttgtaa gtcgattcac ttacagcacc
     3361 tctatcaagg aacttcctca agataacctc tctgcttacc ttgtatatga cagccaactt
     3421 agcaatagaa gcctcgtcaa ctctcattcc tctcacagat aagtcaaaat catcggatgg
     3481 aacaagaatc tcggcagcaa gtctattgca tagtatttca atatctctat cctgttcaga
     3541 taatatgtta acaaaatcat cattcagttt gtctatacca cttatttcaa gcaaaaggtg
     3601 ccctaactca tgaaacaatg taaatatctg tcttgtttta gccattgagt tgttaataat
     3661 aattagaggg aatactttat catataagca gaaaccagag aaatcatcat tcctaaatgc
     3721 ttccttaaaa acaaacacac ccgaatcttc aaaggcctct ctccaggttt ctaatgcagt
     3781 ttcaatagtg tcccaactct tttgtttact taaagatata tccaggtatt ctcttatata
     3841 agttgctatc tctttaagat catggccaac tatatccttc aagtcatttg gcagttgttt
     3901 gcttgaaggg tttactccat tacatagttc ataaagattc tcctgtttaa cccttgcctt
     3961 ccttatctgt ctgattaaag tagaaggtaa tctattgaac tctgcttccg taattgttct
     4021 aaatgatttc ttaatatcat cttcctttgg tggatgagga aagaagaaca cagctattgg
     4081 tcttttatag acttcataag ccagcttctc aagctgagca tatgatggtg aagctaatcc
     4141 cttttcccat gcttcaatta cttcaaaact cttcttaagt tttaccatta cttcatctac
     4201 tgttaggttg gctgattccc ttgcccacct cataacatct gggtttaccg gagccttcac
     4261 cattttactc acctcttttg aaacatggtc taagggtatt ataccattta tcggtatata
     4321 aaactacaaa gataagatta ttcgtaatat atatcccaaa ttcaaatcac aatcgaattt
     4381 atccccgaat aatgggtatt atacataaag aagcagatgt actaactgcc tctttatact
     4441 attagaacaa attacgcatt agtaaatcgt ataagataaa ggaagtgatg cccacatgtc
     4501 aactaataaa caagccaata gactgatcca tgaaaagtct ccctacttac ttcaacacgc
     4561 tcacaaccct gtaaactggt ttccttggag tgatgaagct tttcataaag ccaagttaga
     4621 agataaacct atcttccttt ccatcggcta ctccacctgt cactggtgtc attccgatta
     4681 ctataaccat ttagaaacct tatatatcaa tgcttatgat ctttttaatt ttggctatat
     4741 taaagaacag tgttgatttt attacataat aacaagaggt cagtgtgcct ccttgttcca
     4801 taatataagg attgtacgca tctaaatgtt tatttaatgt tatttttctt tatataagcc
     4861 ataataaatc cacccattgt tgcaatcgat aatattggac ctaattcagc agcattcatt
     4921 ccgtaaccaa tactggtgcc tatcacgcca ataacaatag tcacgataat cccaattata
     4981 atcatcttca tcccctcctt taaatatagt gattttaatt tcatataatt ctacaattgt
     5041 gaaatagcta tataaagatt atatcatttc caacacattt tatcaaatac aatattacta
     5101 ccattactta aaaatcattt taattattga ttaagaatct tcctgtaaag aagatatgat
     5161 tccttactcc ttaataccta attagcccga aaaactgatt tttgtaaatt gggcaattct
     5221 ttatgttgca aggactttag caagtataat agcagaatat ttttatgtgt gggcaaacat
     5281 agactatgag tagattttag agcaagttat ttaaaacgga taaaaaaacc gaaaaattat
     5341 gattgtaaaa attctatgag cttaaatggt aaacagtaag gattctttaa actccctcac
     5401 actataaaaa atatcctgaa ataatgcagc tttttgagtt tattaaacat gtctattttt
     5461 cgagaatatt agtgatctaa attataataa ctgtagtagc aaattaggaa acatagacca
     5521 ttgctatttt ccctatattc atgtttcccg aaatattact gggttcctct gtttatttgc
     5581 gttatgatga tgtgaccaat tgtaatcaac taaattagga ttggcaaaaa aacattggga
     5641 aggtgctgat ggaaaattga gagaaacaac attgcgtcaa attgtatatt ccgcagtcag
     5701 tataatacta aaacaagatg ggttaacaag aacagaatta catgaattgg cagactttat
     5761 acttgacaat agtagtttga atctttccgc agaaaaaatt tgtttggcgg agggaaacta
     5821 ttttggtgtc ggagaatata acaaggatcg gggttttgcc ctgccacgtt ttataacctt
     5881 tgaacttaca cgaccgagtg cttttgacat tatgaataat aataagagta aaacttggag
     5941 gttgcgttcc cacgagacat ttgaagaact tgagaactat ctaacgtatt caaggcaaga
     6001 tcaacctgct gtttttattg atggtaccca tacagaatat aatttaatgg gtacagaaac
     6061 ttggggttat gaagtacaat ggataggctg tgacgctgta tatattgcta catatgtatt
     6121 aaataataaa gaagaaaagg aacttataaa tagtaagctg caactacaaa gaaagttgat
     6181 tgagctcgtg gagcaagggg cagaagaaat tcaagcctat aatgtagtcc agggtgaaaa
     6241 tatgccattc aaagtgcagg gtgttttata taattctaat cagttaaagg ataatcgaaa
     6301 aggtttggtt tgggtaacta agaacgagga tgaatgcgaa gatccttttt aattaagtga
     6361 ttttagcttc agaactatat atatctaagc gaaagacact acatgagggg tagtgtcttt
     6421 tgttttatag aatagcacta cttgaccaat caacgggtat aacatcatcg ttgtctaaat
     6481 tattagcagt aacctcttct atatcgctcc ataaagttcg ctttaatgaa gataattcat
     6541 tttgaatatt ctgtagtaaa agctggtttc tgctaattgt ttgtatcgca ggaggttgtc
     6601 cgttagagtc aagggtctct gggctaccat gaataagctt ttcatattca tttcttatat
     6661 cttcaacaga atttgaagaa ttcaagatat cttcgaaatc atcaaatctt ttttcattaa
     6721 gcagatatat agtgtcatca gcgctgagct gtagaaattt accaatggct gtgaggggat
     6781 cattgtacca gtttccagca tttttaatta aaagctccag agcatttatt actaatggta
     6841 aattgatttc ttttttcagt ttaaaggttt ttaatatctt aaaggcatca gagcttaacc
     6901 ctgtttcttg gaagatagct tgtgtatctg ccttaggttc aacgacctca tctttaacta
     6961 aaaaatctac actcacacct aatccctcgg acagcttaac aacagtatcc attcttggta
     7021 ttgtaccgtt aatatatgag tttatctgtt gcttaggcac accagtaatg ctttcaattt
     7081 cagactgggt taattcggga atacctaaat gttcaaattc accttcagtt attccttcaa
     7141 gtttacgaga aatagcggtt ttgttatatt tgcccatgtt aaatatctcc ttgtataaag
     7201 tgttttagac gattatctca taatattggt aatttatcaa ggattaaaaa cttaagttgt
     7261 catgaataaa tgacaaaatg ttaaacaata tgggtatttc aaataattgt aattatttca
     7321 tgtaagttct tttgaagtga ttgacggcgg aattcatata tgcgatactt aggtcggtaa
     7381 ttaaataatg acagtattag tattaagtaa ttatataatt acaaagttaa aggtggtaaa
     7441 aagcatgacg gaatcggtaa acagattaat aaggtaatac agtagaaaat ttaaaacaga
     7501 aaggggtaaa cgaaatggga gctatgaaaa atgaggggaa acaaagcaag gcagtaaaca
     7561 acgtatttgg catgactggg tttattaacg gtgctgttcc ctttggatat aaggttaata
     7621 aaaataaaga attaggcaga taaaaaagaa atgaaattca ggaggtaaat atgaaaaata
     7681 ttacatttat agtaacggaa gcaacagcaa gggatatggg atatagcgga aacgctgatt
     7741 tggtgagagt taatctacaa gtggatttgt caaggctctc gtataaagca cgaaaacttg
     7801 cagaatcagt atggacctta gaaagagccg atgatttaag taaagtgtac gtaactggac
     7861 ctacccaaag agaattgtat ataaaagatg ggagattaga tgaattaaaa cgctatgaat
     7921 gtatttacgg cagtgaatat tttgataagc caacggttat tgtgtggggt tgggattgct
     7981 taaaaaaaca tgagacacca gaagaatatt ttgaacgtca tgtcaaaatg attggagaag
     8041 caggtggctt cccatttcag tagttggtaa aaggaattaa gaaatgagtt gtatctctta
     8101 ttgatacaac tcaccaaaat taaagattta ggggtatata ttatatggca aatacaatga
     8161 aaacaaagat tagactacct ataagcacca gttcacttga agctataaag aaagagtttc
     8221 agcatatatc agtagaaaaa gatggtgtat atgcgataac cagagggttt gtattacata
     8281 atcttctaaa agcactttca aaagaaaata atgaggagat aattgctgaa cactcatcaa
     8341 gtatcgattg gtattctacg ttatatatag agcgatacaa agatggcaat agcgaaacaa
     8401 ttaaaacaaa aaccagcgag attattatgt atgaaattac tgatccagaa gaaattgagg
     8461 agatattgaa tgagcaggag agttgttaat aaaaatttaa tacctgcact actagcaggt
     8521 gcagtaggac aaacacctaa gcaatcggca accgatttga aaatgctaca tgattacaat
     8581 tttgcattta atataattct aattgcggct gatgatattg tgaatctagg tgataagatt
     8641 gatatgaatt ggtgtataca aactgcgtac gagcttgata acggtccaaa aaaatacttt
     8701 tgggaggtat aacatgtatt caaatgtaag gggaaagttg gtagaaagca aagttatcac
     8761 aatagcgccg aaagggctga aggagtatat agttaataaa ttggattctg gtttagagat
     8821 taatgaaact caaatatgga aactaagggc aagaacattt gaaaaacaga ggttatcaat
     8881 ggtaaaattt ttgaggatga ggatattttt gcctttgcaa gattcattga gcttcaacta
     8941 tggcaggaat acaacgtctt atcagatata gttcaagcaa tattctatct tggtgatggg
     9001 tcaagtcata ataaaatgat agcattggat caggtaagaa cggcattaga tattactttg
     9061 attttgatta tgaaagccag ggcaagaaag aaacaaaggg gtaattcata tgttaattac
     9121 atacaaagct aataatgatg aaatcagatt cgctgatcct gatattaacg agctcagtaa
     9181 ggaacaagtt attattgaac tgggaaaatc caagttcaga ctaaataagc tgcaacagca
     9241 aatagataga gaaaaggcac tgcatgaaaa acttttaaag cggcgccttg aattctataa
     9301 gtatgaaaag gcggaagaac taaaagcaaa taacaatatg aatctttcca gcaaaaagag
     9361 ccagattgaa ttgtactact cacagggtaa gggctatgat gagattgctc tgctcacaga
     9421 agcctcccct gcctatatct ataatgtggt atgcagctac agaaagaaaa atactgtacc
     9481 aatcaaaaac ccagaaaaaa cagaaataga agaagccata ataatgtata gtagagttaa
     9541 taaaaacatt aaagagattg ctaaaaattc aaatgtaagc aggcagtatg tgtataaggt
     9601 ccttaaaaag cacaatatcc cctacccttc tataaaaaga aagtaagtta gaacgagttt
     9661 acaagattga atgttagggt agtttacaaa gagtaaaaaa taaaggttaa aatagatcga
     9721 tcccctcact tttagcaata ctaaatcaac gaaatgcaca aacgacaact gaacgcttga
     9781 ttttggaact aaaaaagaaa ttaggaaata taaattaaaa aataatacct gattggttat
     9841 agaatagttc aatataatca atataaaact gggtattgta aaaaatgaat actacagtta
     9901 taaaaaagta atagacataa ataaagatta atagaagtaa tgaataagag agaaaaatgg
     9961 agaaaaattt ttcggaggtt caaagttgaa caaaaattct cggaggttca atacaaggcg
    10021 aaaagtagaa atgctaatag gatggatcga tgtaaatagc gatatatcag ctgtgagtag
    10081 ggtcaagtag tattaaaaac agaatataaa gtggattttt gggatagtgt ttcatagcac
    10141 tatctttttt aatatcaaaa atatctcaat cgatagcaaa agtattgaaa aagcaagacc
    10201 tatgtgatat tatatatagc aatttatggg aggtacgctt atatgggatt tagttatgac
    10261 aagctctgga agcttctaat agataagaaa atgaaaaaag tagatcttgg gaataaggct
    10321 gctattggcc caaatacaat ggcacgactt tctaaggggc agccagttca aatgaatgtg
    10381 ctgggcaaga tttgtaagga attgcaatgt aatattggtg atattgttga ttatatagac
    10441 gaggcaggtg gtgatagtaa atgagtgcaa aagaagccaa ggcgagaatt aagataaaca
    10501 aattacttga agaagctggc tggagattct ttgatgagga tggaaaacca gcaaatatcc
    10561 aagtagaacc taatgtaaaa atctctaaaa aacaagtaga tgatatgggt gaaaattttg
    10621 ataaggtcaa gaatgggttt attgattttt tgttgcttga cgacaaaggg catccattta
    10681 ttgtgcttga agccaaatca gaggataaag atccgttggt gggtaaagag caagcaagag
    10741 aatatgcaaa atcactattc ataaagtatg ttatcctttc aaatgggaac cagcattatt
    10801 tctggaatat ttacaagggt aatccacaga tcatcaccag ctttccgaac tgtgaaagca
    10861 tcagagaaaa taaagctttt aatgcggatc catcaagatt atataatgag gatgtaaaag
    10921 aagattatat tgcggtagtc aaaaatccga gatatgcgga agcaccagaa tatcaaaatc
    10981 ctgaaacaag aaaaaagttt ctaaaggaca gtggattaag atttttaaga ccttatcagc
    11041 tcaatgcagt taaagccttg cagaaatcag taaaaaaagg taaccagaga tttttgtttg
    11101 aaatggcaac aggaacaggt aaaactttaa cgtctgcagc agtaattaga ttgtttttaa
    11161 gaagcggaaa tgctcatagg atattatttt tagtagatag aattgagttg gaaaatcagg
    11221 caaagaaaaa ctttcagaat tatctatccc ctgattatca caccgttgta tttaaggaaa
    11281 gtactgaaga ctggcgaaaa gctgaggttg ttgtatctac agtacagacc ttaatgttca
    11341 ataataaata taagaagtat tttaaaccaa ctgacttctc actgctcatt gttgacgaaa
    11401 gtcacagatc aatcaatgga aatagcagag cggtatttga atattttctg gggtataagc
    11461 tgggccttac agcaacaccc aaagattata ttaaaaatgt tgatatagat aagttgaaat
    11521 caaccgaccc aagagcatgg gaaaaaagac aattgttgga ttcatataag actttcggtt
    11581 acgaaagcgg tgatcctaca ttcagatatt ctctattaga tggtgtaaag gatgggtatc
    11641 ttataaatcc aacagtagtg gatgcaagaa cagacattac cacacagctt cttgcagatg
    11701 aggggtatgc cgttataaac caaacagaag ataatgacga tgaagaagaa gtttactatg
    11761 caaaggattt tgagaggaaa ttcttctcag aggaaaccaa taaaatattt gtaaagacat
    11821 ttatggaaaa tgcaaagagg gaccctttat caggtgaaat cggaaagagt attatttttt
    11881 gtgtaagccg taagcatgca gctaaaatca cacagatact aaatgaattt gctgataaat
    11941 tgttccctga taaatattac tcggattttg ccatgcagat aacttctgac gtaatgaatg
    12001 ctcaaactta tacaattaac ttccagaaca ataatttaag tggaactaca aagtttttag
    12061 agaattataa atcctcaaaa acaagagtat gtgttactgt gggtatgatg acaacggggt
    12121 acgattgtga ggatttactg aatatcggat tattaagacc tatcttctct cctactgatt
    12181 ttatccagat taagggcaga ggcacaagaa agtttgaatt taactataag gacaaaatcg
    12241 ccggagaaat acaagaacac aaattcgaaa aggatacatt taagttgttt gacttctttg
    12301 gaaactgcga gtattttgag gaaaagttta attatgatga agtaataaat ctgccaaagc
    12361 cacagagtaa aggagcagat actaaaacac caccagtaga tggcacaatg tatgaaaact
    12421 ttgacccaga ccctttaaaa tttatcaaag aaacacctat tggctatgag ggtatgaaaa
    12481 ttgataggat gttctttgat aaatttgaaa gcaccgtcaa agaagatgaa acagcgaaag
    12541 agcaatatga acaaggcaac tatgctgcag ttcaacatta tattgaagaa cagattatga
    12601 acaagccttc agaatactat acttgggata agttaaaacg gtccattggt attgataggc
    12661 gggtaacagt aaaagaaatt cttgataaaa tatttggagc aattccagcc tttaagtcaa
    12721 agagagaatt aatagaggat gaatttgaaa gttacttact aaccagacca atacaagccg
    12781 ataaatatac ggaaataaaa agattctttg aaacctatat catggataag gatgttagaa
    12841 gaattataga agatgaaaag tatcaacttc ttggtacaga ggtcagcacc tataccatgt
    12901 cagacttaaa gcgattaggg gtagataata tgagacaggt tgttgactat attaatgaca
    12961 atgtaaatgt tacaagattt gttgcatagg aggatcagaa tgttaaactt tgatgttaaa
    13021 agaaagataa atactttaag agatatatta gtaggtaagg taccagaccc aaaggcacag
    13081 gtagaacaga ttacaatagc tttaatctat aagttcatgg atgatatgga tatagaggga
    13141 atggaatttg gtggcagcag ggaatttttt aaggaagatt atgaaaaata tgcctggaca
    13201 aatataatgc agccagaaaa cagcggccag caaagggcta atttgtatgc agagggcata
    13261 gataaaatga gcatgaatcc tcatttgcca cagttattca gagatatatt cagaggagct
    13321 tatgttccct atcgtgatgc agaaacatta aatatgttcc tgaaagaaat taatgatttt
    13381 agttatgacc atagtgagga attgggcaat gcttttgaat atctgctttc tataatgggt
    13441 agtcaaggcg atgcaggaca atttagaaca ccaagacata tcattgatat gatggttgag
    13501 atagttgatc cgcaaaagac ggatagcata cttgacccag cctgtggtac atcaggattt
    13561 ttaattagtt cctataagca tattttagag aaaaacaaag ataatgacgg aaacagcaca
    13621 ctgtcagctg atgagagaaa gagaatgact gaaaactttg caggatatga tatttcacct
    13681 gatatggtta gattatcaag ggtgaatatg tacctgcaca agttcgcaaa accgcagatc
    13741 agtgaatacg atactctttc cagccttgat aaatgggatg aaacctatga tgtaatttta
    13801 gctaatccac cgtttatgac accaaagggc ggtattatcc ctcacaacag atatagaatt
    13861 ccagcaaagc gtgcagaggt attgtttgtt gactatatag cagagcatct taacccaaca
    13921 ggaaaagcag gtattattgt accagaggga attgtattcc aatcagctaa tgcttataaa
    13981 tcgcttagaa aatatttagt tgaagatgat ttactttatg cagttgtatc tcttcctgct
    14041 ggggtattta atccttatag tggtgtaaaa acaagcattt tactgatgga taggaccctt
    14101 gcaaagcaaa agaatgaaat cttatttgta aagataaata atgatgggtt tgaccttgga
    14161 gcacaaagaa gagaagtaaa aggtagtgat attccagagg taatcaatat tattaaggat
    14221 tatcagaatg gaatcgatat atcagataat acgcttgtaa caatagcaag taaaaaggat
    14281 attgcagtgc aggattatat tttggttggt gagagatata aggaagctgt tgttgttaat
    14341 agtgattatc cattagtaga attaggcgag atatgtttgg ttgagcgagg aacttccatt
    14401 acaagtaaag aattaataga tggagatatt cctgttattg caggaggaca acaaccagct
    14461 tattttcata atatttccaa tagaactggt aaagttatta cagtaagtgg ttcaggtgca
    14521 tatgcggggt ttataaacta ttttgaagaa cctatttttg catccgattg ctcaactatt
    14581 cagtcaaaca attcaaatgt aaatttaaca tatgtatatc atacaattaa ggctcagcaa
    14641 gatagattct atgaattaca aagtggaatg ggccaacctc atgtgtatgc aagagatatt
    14701 aaaccattta aaatccctct tccaccttta cacatacaag aggaaattgt aaaggaaatt
    14761 gaggggtatc aaaagattat tgacggtgca aggcaggttg ttgagaatta taagccgact
    14821 atcaaaatag acccaagctg ggaaatggt
//
LOCUS       DAOSDD010000097        14782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1617_length_14782_cov_4.849189, whole genome shotgun sequence.
ACCESSION   DAOSDD010000097 DAOSDD010000000
VERSION     DAOSDD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14782
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1617_length_14782_cov_4.849189"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PK733_09965"
     CDS             <1..1360
                     /locus_tag="PK733_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ10902.1"
                     /translation="LSGNKEMLEKIDLFDNAIIDGHSTGISAKELCAYRSVGVMTNHE
                     CTSIEEVLEHLRLGMYVQIREGSGARNLESIVKGMLERGQSFDHCLFCTDDKHLEDIR
                     QEGHISYNIRKAIQCGLDSIEAIRMATLNPAQCYKLKRLGAIAPGYSADMLLLSDLEN
                     FTVEKVLFRGKVISERGSKPYIDVSVQDRNVFNTVRIQPVTAEDLKIKIKGCEPHKAE
                     VNESCKSEIAKSLRAGINEPCEIGVITVIPNQLITKKEMVKVQTDKEGFLILDSILDH
                     DNIILDNDSKLNVNNNLNPNSRLNKVAVIERHKATGCIGLGIVKGFGIRGGALASTVA
                     HDSHNLIVVGDNDEDMLAAIDELKRVNGGMTVVSDGKVLGTLPLPIAGLMSDHDSDWV
                     EKKLASMMQSVRELGVREGVDPFITMSFLALPVIPEIRVTDRGLFDVSRFEFINITYR
                     RE"
     gene            1480..2763
                     /locus_tag="PK733_09970"
     CDS             1480..2763
                     /locus_tag="PK733_09970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ10903.1"
                     /translation="MNRRLLSFVTYTGYFIIGIVSFIISPTLPVIIKDFNITKGVAGA
                     IFTANAAGNFVGVLAGGFFSDLIGKKPLVVLGCLLQIIGFTFTAITGSWALVLGFFFI
                     TGMGRGFLNTCFNALIADINTHRRGAAFNTLHGIYGAGCLIGPALAGLMLSLDYEWRV
                     VYYCAAALWMLYMIAIIPITYPAASTVNNDVNNSVHSPAKGLAAWNSKKTYSKRISLK
                     NSDINDFSIRTILFSPAFIMLFIVSFIYNSSATGLIGWINTYLEGMEFSILLGAGMVS
                     IFYLGLTIGRFICRFLSEKVGYSKTILACAIGCFVFYPLIIYATNSTLIAAGVFFSGL
                     FFSGLHPTGLAYANKLFPSISGTITGLLSTAMSLGAMSLPWLIGLVAEKSDFKAGFSI
                     GFVLIIILLGVAISLLLYEKKHRNGSQNMRYITQK"
     gene            complement(3167..3538)
                     /locus_tag="PK733_09975"
     CDS             complement(3167..3538)
                     /locus_tag="PK733_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ10904.1"
                     /translation="MKRSMMQVFVKGSKEALELYRKGFDTEVLCTYPDDNGGYMHTEL
                     NAYGQEIAISEINQNVIPGNTMMFCFHFGEGGESKVKKAYAMLKDGAAVNNPPEACDY
                     SPCQCVLTDKFGVTWCLFENV"
     gene            complement(3666..4127)
                     /locus_tag="PK733_09980"
     CDS             complement(3666..4127)
                     /locus_tag="PK733_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOJ10905.1"
                     /translation="MNKSICGLDCNGCELKDTCGGCVATNGRPFGGECVIAVCSQSKG
                     QEHCSKCSDSLCNLKKQLIAEFNALGIEDMAEVTDLNALKGSFINLEYMLPSGQAIKF
                     WDDDRIYLGNQICKKDSDRCYGLTADENYLLVCEYGNSGSDAEIIIYKKKR"
     gene            4485..4844
                     /locus_tag="PK733_09985"
     CDS             4485..4844
                     /locus_tag="PK733_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ10906.1"
                     /translation="MDNLKLFDAEYRFMCVLWEHEPVNSTELVRLCFDRFEWKKSTTY
                     SMIKRLGERGVLKNENAIVMALVKKEDVQKYESEVLLEKTFDNSLPVFLTTFLRDKKL
                     SKSEAEKLKKMIEEATK"
     gene            4841..7576
                     /locus_tag="PK733_09990"
     CDS             4841..7576
                     /locus_tag="PK733_09990"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ10907.1"
                     /translation="MTNVFITVLNMSLIASFVALGVILIRILLKKAPKVFSYALWAIV
                     LFKLLFPFTIETALSLIPMKAEPVPQNIIYSQTPSIDSGIPFVDHSINQVITNTVPPA
                     NPAASVNPMQVILFVLSCIWLAGIAVLLLYSLVSYFRLKYRLRVAIFIQENIYETDRI
                     KTAFVLGFVGPKVYIPVGLSAQEIDYILKHEQTHIRRRDYLIKPLAFLAVCVHWFNPV
                     IWFSYYLAMRDMELSCDESVLKHYGSDIRRDYSNSLLSLSVKQIALVSPLAFGETGVK
                     DRIKNVLNYKKPAVWVSIMAITAVIVAAVLLLPNAVQDTKDISDEFSDTTTENITLEY
                     SDESGVYTENYIKKTTDDSVTLFTEDSKEIISGEDIIPLPYIYAVKTNGQYDLLDKGT
                     HAKVSNISYDEIFCEKYPDGRLSTTIMKGRVGKYWGLISAKGKTLTNLQTQTFEDIQI
                     NTYEEAWPIIAVIEGGKYGAIDYNGKTVIEAEWGYLAMDVYNVPDTVFVFDGSKWGGI
                     KLDKNLLASTVDYSLIPPEWIIQNYNQMLLTSSSVNSTQLASMFDFADKYRFDYVPFF
                     TENNVPQSSSEYLYYAFVINLDNWGADKGIMSKTHVENVIKAHFEVKTIVHEALRRAW
                     NFDGINYTAVPMGIKDKPVYVLTDLRVSTKDGKTVYEAAMNNCNLADGSLASDEDRVK
                     IKAEIMNGDYSSLVSLQRETFTYYLNDNGEPVFIAHIMTDRPEMKWYYQMFRNDTAFK
                     VLGSTSGDFTDDQMATYAILKMAHHYSYENGNTKEEYNAITQKYFGRDIKNFNNGSTE
                     IIPGTDKIRATGWSYNSSMYVIVKDIWDNGDGSMTGDFYCLNISDSHWTGKDGEFQEA
                     EYALLNGDISSFTDCDISIKRVVYEVKAENEKQYFKYRSVKNLEENVKSIVLYSK"
     gene            complement(7979..8200)
                     /locus_tag="PK733_09995"
     CDS             complement(7979..8200)
                     /locus_tag="PK733_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10908.1"
                     /translation="MYSIKFSNVQSLLYDKGILLLSREICKNKINLISGVYPVQLLPH
                     LLKLYGHLLRMILKLWKGLLLFSLLFIRS"
     gene            8606..8980
                     /locus_tag="PK733_10000"
     CDS             8606..8980
                     /locus_tag="PK733_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ10909.1"
                     /translation="MVWQFKADRPIYTQLVEQIELRIVSGEYSPGSKLPSVRDLAAEA
                     SVNPNTMQKALAELEQNGLLYSQRTSGRYVTEDENMIRKVKDTIALEEIKDFLQKMSK
                     LGFNSQETISLVERIIKEMIPQ"
     gene            8977..9675
                     /locus_tag="PK733_10005"
     CDS             8977..9675
                     /locus_tag="PK733_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10910.1"
                     /translation="MNPILECRALTKKYGNTQALSDVNLTIERGRIIGLLGPNGSGKT
                     TLIKLANGLLTPTGGEILIDGKHPGIETKKIVSYLPERTYLNNWMCVREVIDFFKDFY
                     SNFNPEKTYEMLKRLAINPDDKLKTLSKGTKEKVQLILVMSREADIYLLDEPIGGVDP
                     AARDYILNTIISNYNENAAVIISTHLIADIEAVLDDVVFLANGTIALAEPVDKIREEQ
                     GKSIDGLFREVFKC"
     gene            9669..10487
                     /locus_tag="PK733_10010"
     CDS             9669..10487
                     /locus_tag="PK733_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ10911.1"
                     /translation="MLGKLFKYEIKASARIFLPLYASLILFAIINNIFIRINNSSNNN
                     QLFMNIMQGISTFAYVFLIISIFVFTLFVMIQRFYKNLLGDEGYLMFTLPVRTGEHIA
                     AKLLASMMWVITSVIMTLFSVMLIAYNEKFFASFQEAISQILTEINRVFGVNGYAYIV
                     EFAVFILMVLATNILMIYASISVGQMISKHKLLGSFGAYMGFYLISQFIISIILVIGY
                     SAGSRHWTSIENIMQNNTTIPHFLFAGIMLLNLVFAAAYYTITNLILSRRLNLE"
     gene            complement(10776..11543)
                     /locus_tag="PK733_10015"
     CDS             complement(10776..11543)
                     /locus_tag="PK733_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ10912.1"
                     /translation="MSKVIQFSRRNKLLILVALSYILLSIFMPDKAVQSLKNSLYYIK
                     EMLMIMPVILLLTALITAWVPKKAIEKNLGKDSGIKGSIFSFLLGSFSAGPIYAAFPV
                     CKALLSKGASISNIVILLSTWAVIKVPMLLNEAKFLGPKFMIVRWILTTIAIFIMGYV
                     TSKLVKKEDLPLDDIKENSSVDTLNISSEYCIGCGLCAKIAPEYFKVENKKAKVIKQC
                     IKQDIKQDIKQGEMKIVNEAIEKCPSKAINQNKQVNF"
     gene            complement(11540..12022)
                     /locus_tag="PK733_10020"
     CDS             complement(11540..12022)
                     /locus_tag="PK733_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ10913.1"
                     /translation="MDVFTLAFWIITLIWFIGSIIKDKKKTIDSVKMSSGMMKHIAGE
                     IVAILFLIGLILTFLPPETIKQYAGQSNSIVTTIVFALVGCITLIPAFVAFPLAGSLV
                     NAGASIVPVVAFLTTLTMVGIVTFPLEKKEFGTKFTLIRNSLSFIFAISIALIMGVIL
                     "
     gene            12330..12923
                     /locus_tag="PK733_10025"
     CDS             12330..12923
                     /locus_tag="PK733_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ10914.1"
                     /translation="MFNSSNYRVGKYDKGQVLHIQNELCESMDMILEGKLSVQKIDEE
                     GNVLKVAVFSDGDILGANLLFSNSNSYPMTIVSESKTAILHIYKELILELSQNNVTFT
                     SNLLTIISNKTLVLTDKIDAISLKTIRQRINDFLKYEYHIQKSNVIKLNISKKDLAER
                     LGIQRTSLSRELNKMRKDRLIDFDSKTITVKDAKYLV"
     gene            13215..14111
                     /locus_tag="PK733_10030"
     CDS             13215..14111
                     /locus_tag="PK733_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10915.1"
                     /translation="MSFNDLINRIPANIHARTKTLISGDIAVFKPEKYVIESKIRMND
                     YHFIIFHSTPPMAKIGDREFQFKKGSLVSLEPGTEITVYPHEKNQQCQYMSISAKKNF
                     VERIAFEAFGRDKIKFDKLENTYSKQLLESINNFEFELTNFGDKYPIMLQSIATQIAF
                     LLLRDTFAGSQRYSEMSITNDDDYVAKAIDYMKKYYSCNITIDGICRAIYLSPSYFSR
                     KFKNKTGMTPYKFLLELRLAKAKELLSEGDFSIEEASRLCGFANSAHLSTAFKKSEGL
                     SPSEYRKQFQYIHIHDMKNQQR"
     gene            14407..>14782
                     /locus_tag="PK733_10035"
     CDS             14407..>14782
                     /locus_tag="PK733_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10916.1"
                     /translation="MFKKVVPAIICLALFLTNTLFTMAEHQNIPAPENLTAEILNYSD
                     GTPYFKITFDTPSSLKALAGKEYEYNEAGGNYFIELEFKIGDGSWYSGGSGFSYEAWQ
                     NVNLGPEDVGVDGNIDIKANTYH"
BASE COUNT         4780 a         2514 c         2894 g         4594 t
ORIGIN      
        1 gctttcagga aataaagaga tgcttgaaaa aatagattta tttgataatg ctataataga
       61 tggacactcc acaggtattt cagcaaagga actttgcgcc tatcgctcgg taggagttat
      121 gacaaaccat gaatgcacta gtatagaaga agtattggag catctgcgtc ttggaatgta
      181 tgtccagata agagagggtt caggagcaag aaatcttgag agcatcgtta aaggcatgct
      241 ggaaaggggg caatcttttg accattgcct tttctgtact gatgacaagc atctggaaga
      301 cataagacag gaagggcata taagttataa tatacgaaaa gctatacagt gcggcttaga
      361 ctcaattgaa gccattcgta tggcaaccct gaatcctgcc caatgctata agctcaaacg
      421 tcttggagca attgctccgg gatattcggc tgatatgcta ttattatcgg atcttgagaa
      481 ctttacagta gagaaagttt tatttcgggg caaggtgata tcagaaagag gaagtaaacc
      541 ttatatcgat gtttcggtgc aggacagaaa tgtttttaat acagtccgca tacaacctgt
      601 tactgctgaa gatttgaaga taaagataaa aggttgtgag cctcataaag ccgaagttaa
      661 tgaatcctgt aaatctgaaa ttgctaagtc ccttagggca ggaattaatg aaccttgtga
      721 aattggggtt attactgtaa ttccaaatca gctgattaca aaaaaggaaa tggtaaaagt
      781 acaaacagat aaagagggat ttctaattct tgatagcata ttagatcatg ataatattat
      841 attggataat gatagtaagt taaatgttaa taacaatcta aaccctaata gcagattaaa
      901 taaagttgct gttatagaaa ggcacaaagc cacaggctgc atagggttgg gaattgtaaa
      961 aggctttggt atcaggggcg gtgctttagc ctctactgta gctcatgatt cccataatct
     1021 tattgtggta ggtgataatg atgaagatat gctggctgcc atagatgaac taaaacgagt
     1081 caatggtgga atgaccgtag taagtgacgg aaaggtactg ggtacattgc cgctgcccat
     1141 agccggacta atgagtgacc atgacagcga ttgggttgag aagaaactgg caagtatgat
     1201 gcagagtgtc agagaacttg gggtgaggga aggagttgat ccatttatca ctatgtcctt
     1261 tttagcattg cctgtgatac cggaaataag ggttacggat agagggcttt tcgatgtatc
     1321 aaggttcgaa tttataaata taacatatcg cagagaatag ctgtaaacaa ataaatgtgt
     1381 gctgttggtt acagcataag tttacaagaa taggaaaaca gtagagcagt atttttcaaa
     1441 aaacttaacc taaagatatg tgtttgagga gggctagttt tgaatagacg acttctttcg
     1501 ttcgttacat atacaggcta ttttattata gggatagtgt cttttataat ttcacctaca
     1561 ttgcccgtta ttataaaaga tttcaatatt acaaaaggag ttgcgggggc aatttttact
     1621 gcaaacgcag caggaaattt tgtaggtgta cttgcaggag ggtttttctc agatttaata
     1681 ggtaagaaac ctctagtagt tctgggatgc cttttgcaga ttataggatt tacattcaca
     1741 gcaataacag gaagctgggc tttggttctg ggtttctttt tcataacagg tatgggcagg
     1801 ggtttcctta atacttgttt taacgcttta atcgcagata taaatactca tagaagagga
     1861 gcagcattca atactcttca tggaatttat ggtgcaggtt gtcttatcgg tccggcactt
     1921 gcagggttga tgttatctct tgattatgaa tggagggttg tatattattg tgcggcagct
     1981 ttatggatgt tatatatgat tgcaattatc cctataacct atcctgctgc aagtactgtt
     2041 aataatgatg tcaacaactc tgtgcatagt cctgctaaag gtcttgccgc ctggaattca
     2101 aaaaagacat attcaaaaag gataagtcta aaaaatagtg atataaatga tttttcaatt
     2161 aggactattt tattcagtcc ggcatttata atgcttttca tcgttagctt tatatacaac
     2221 agctcagcta cgggtctgat cgggtggata aatacatatc ttgaaggaat ggagttttct
     2281 atccttttag gggcaggtat ggtatcaata ttttatctgg gtttaaccat tgggagattt
     2341 atctgcagat ttctatcgga aaaagttggg tactcaaaaa ctattctagc ctgtgcaata
     2401 ggttgttttg tattttaccc tttaataata tacgcaacaa actctacttt aattgctgca
     2461 ggtgtatttt tcagcggctt gtttttttca ggacttcatc ctacaggtct tgcatatgca
     2521 aataagctct ttccatcaat atcaggaaca ataaccggac ttttatcaac agcaatgtca
     2581 ctgggagcaa tgtctttgcc atggctaata gggcttgttg cagaaaaaag tgactttaag
     2641 gcaggttttt caattggttt tgtgttaata attattctgt taggagttgc tatatcactg
     2701 ttattgtatg aaaaaaagca cagaaacggg agtcaaaata tgcgatatat aacgcaaaaa
     2761 tgattttcaa tcaagaaaat atgcgtagct ttgcttagcg taatgacagg ggtatatcca
     2821 tctaacttag tcgactgtgt ccagaaagta gaaatggatt tacagtaagt aatgacctat
     2881 attagaaatt ttcactcctc aaaatcaata tatgggaata ttctattcta ttctttactt
     2941 tacctatttg aagctttgga gatagatatc ccctaccttt tcatgcagct tccaattcac
     3001 atacaggtga ggcaaatata actcctcttg ttataccatt aatgaatttt tgcgaataag
     3061 tatttttgaa gcaccttact aatactaagc aaatattaat gaagtaaacc ctactatttt
     3121 aaaatctttc gccgtaatat ccttcaatat cggttctctg atagattcat acattttcaa
     3181 acaggcacca tgtaacgccg aatttatcag tcaggacaca ttggcacggg ctgtaatcgc
     3241 aagcttccgg tggattgttt acagctgcgc catctttcag catcgcatat gcctttttta
     3301 ccttgctttc gccgccctcg ccgaagtgaa agcaaaacat cattgtgtta ccgggtatta
     3361 cattttgatt gatttctgat attgcaatct cttgcccgta ggcgttaagc tcggtgtgca
     3421 tataaccgcc gttatcatcc ggatatgtac acaatacttc tgtgtcaaaa ccttttcggt
     3481 aaagctccag cgcctcttta cttcctttta caaatacctg catcattgat cttttcattc
     3541 tattctccta tgaaaatttt aagatacgga ttaccattcg tttcgaaaca gccgccacat
     3601 ccgcaagtta ctttgtattc gcagcctgtg cagtgaagtc cacatcttga atcaaccatt
     3661 atgtattacc tcttcttttt gtaaatgata atttccgcgt cagaaccgct gttgccatat
     3721 tcgcaaacta aaagatagtt ctcgtcagca gtcaagccat agcacctgtc gctgtctttt
     3781 ttacagattt ggttgcccaa ataaattctg tcatcatccc agaatttaat cgcttgtccg
     3841 cttggaagca tatattcaag gttgatgaaa gaacctttaa gagcattaag atctgtaact
     3901 tctgccatat cttcaatacc gagcgcatta aattcggcaa tcaactgttt ttttagattg
     3961 caaaggctgt cagagcattt gctgcaatgc tcctgccctt tgctctgaga acatacggcg
     4021 atgacacatt cgccgccaaa cgggcgtccg tttgttgcaa cacaaccacc gcatgtgtct
     4081 tttaactcac acccgttgca atccaatccg catattgatt tgttcattat gtattacctc
     4141 cgtttttgct tttatgatac cacaccgaac acgacaacaa aatatgaaaa atacttataa
     4201 atatgttaca acaaaattag aagacacgcc agaagggagc ggcattcttg gattcagaaa
     4261 cctgtagtct catggcatac accgagtgca aattttattt atgaagaatt tatagacaga
     4321 tgcattagtg gaagctttat gtattgtaac gatagcttct ggaatatatt tcttcataga
     4381 ttttaaatgc accccaaaac agagatttct attgacaaac gaggtcggat gcgttagact
     4441 aacaataagg tctgatgcat tagaccttat tgagggggct aaatatggac aatttaaaat
     4501 tatttgatgc cgaataccgt tttatgtgcg ttttatggga gcatgaaccg gtgaactcta
     4561 ccgagcttgt aaggctttgt tttgaccgtt tcgagtggaa gaaatccacc acatattcta
     4621 tgatcaaaag actcggtgag cgcggtgttc tgaaaaacga aaatgccatt gtgatggctt
     4681 tggtgaaaaa agaagatgtg caaaaatacg agagcgaagt tttgcttgaa aaaacctttg
     4741 acaactcgct accggtgttt ctgacaactt ttttgcggga taaaaaactc tctaaaagcg
     4801 aagctgaaaa gctcaaaaaa atgattgagg aggcgacaaa atgaccaatg tatttattac
     4861 cgttcttaac atgagcctta tcgcctcctt tgttgcgctg ggcgttattt taattcgtat
     4921 tctgctcaaa aaggcgccaa aggtattttc ttatgcgctt tgggcaatcg ttcttttcaa
     4981 attgcttttt cctttcacta ttgaaacggc gttgtctctt atccctatga aagccgagcc
     5041 tgtaccgcag aatatcatat attctcaaac tccgtccatc gacagcggca ttccgtttgt
     5101 ggatcattcc ataaatcagg tgattacgaa tacggttcct cctgcaaacc ctgccgcaag
     5161 cgtaaaccca atgcaggtta tattgtttgt gctttcctgc atatggcttg ctggcatcgc
     5221 ggtgctgctt ttgtattctc ttgtttccta ttttcgtttg aaatatcgtt tgcgtgtagc
     5281 aattttcata caggaaaaca tttatgaaac cgaccggata aaaacagcct ttgttctggg
     5341 ttttgtgggt ccgaaagttt atattcctgt tggtctttcc gcacaggaga ttgactatat
     5401 cctaaagcac gagcaaacgc atatcagacg gcgcgattat cttataaaac cgcttgcctt
     5461 tcttgcagtt tgtgtccatt ggtttaatcc cgtcatatgg ttctcatatt atcttgccat
     5521 gcgtgatatg gagctttcct gtgatgaaag tgttctgaag cattacggca gtgacattcg
     5581 tcgtgattat tccaactcgc tgctgtccct ttcggtaaag caaatcgccc tggtttctcc
     5641 gcttgctttt ggtgaaaccg gagtgaaaga cagaatcaaa aatgtgctga actataaaaa
     5701 gcccgccgtt tgggtgagta ttatggcaat tactgcagtt attgtggcgg cggttttatt
     5761 actccccaat gctgtgcagg atacaaagga tatttctgat gaattttctg atacaacaac
     5821 agaaaatatc accctcgaat actccgatga gagcggagtc tatacagaga attatattaa
     5881 gaagaccaca gacgattcgg tgacattgtt tactgaagac agcaaagaaa tcatttctgg
     5941 cgaggatatt atccccttgc cctatatcta tgcggtaaaa acaaatggcc aatatgatct
     6001 gctggacaaa ggaacccatg caaaagtgtc aaacatttct tacgacgaaa ttttttgtga
     6061 aaagtaccct gacggaaggc tttccacaac aatcatgaaa ggccgggtcg gcaaatactg
     6121 ggggcttatc agtgcaaagg ggaaaaccct cacaaatctg caaacccaaa cctttgagga
     6181 cattcagatc aatacatacg aagaggcatg gcctatcatt gccgtgatag aaggcggtaa
     6241 atacggtgcg atagattata acggcaaaac ggtcattgaa gctgaatggg gctaccttgc
     6301 aatggatgtc tacaacgttc ccgatacagt ctttgttttt gacggcagca aatggggtgg
     6361 cataaagctt gataaaaact tactggcgtc aaccgtagat tatagcctga taccgccgga
     6421 atggataatc cagaattata atcagatgct attgacctca tcctcagtca atagtacgca
     6481 gcttgcaagt atgtttgatt ttgcagacaa atatcgtttt gattatgtac cgttttttac
     6541 cgaaaacaat gtaccgcaaa gcagcagtga gtatctttat tatgcttttg taatcaattt
     6601 ggacaattgg ggtgccgaca agggcataat gtcaaagaca catgttgaaa atgttattaa
     6661 ggcacacttt gaagtaaaaa ccatagttca tgaagccctg aggagggcgt ggaattttga
     6721 tggtataaac tacaccgctg ttccgatggg tattaaagat aaacctgttt atgttcttac
     6781 agatcttaga gtaagcacaa aagacggaaa aactgtttat gaagctgcga tgaataattg
     6841 caatttagcc gacggaagct tagcaagtga cgaagatagg gtaaagatca aagcggaaat
     6901 aatgaacggc gattattctt ctctggtttc attgcagagg gaaacattta catattatct
     6961 taatgataac ggtgaaccgg tatttatcgc acacataatg accgatcgtc ctgaaatgaa
     7021 atggtattat cagatgttca gaaatgacac tgcatttaag gtgctcggca gcacatcggg
     7081 cgactttacc gatgaccaga tggcaaccta tgcgatttta aaaatggctc atcattacag
     7141 ctatgaaaac ggcaatacaa aagaagaata taatgccatt actcaaaaat acttcggccg
     7201 ggatattaaa aatttcaaca atggttcaac cgaaataata ccgggcacag ataaaatcag
     7261 ggctacaggc tggagctata acagcagcat gtatgtaatt gtaaaggata tctgggataa
     7321 cggcgacgga agtatgacag gcgattttta ctgcttaaac atttctgatt ctcattggac
     7381 aggtaaggac ggagagtttc aagaagcaga atatgcattg ctaaatggcg atatttcttc
     7441 ttttactgac tgcgatatca gtattaaaag ggttgtttat gaagtgaaag cagaaaacga
     7501 aaagcagtat tttaaatacc gtagtgtgaa gaatcttgag gaaaatgtaa aatcaatcgt
     7561 tttgtacagc aagtaactta atgctatgag aaaacccctt atttatcatt aatcacaaaa
     7621 gcagatggcc acaaaattgt ggatggccat ctcactggat tcgggtaaaa aagattcccg
     7681 gctcaggagg agccatttgc cgtgcctatt gtgataaact cttccggtta gaacgaaaat
     7741 ggaaagagct gatcccggaa gaacggaaaa agaaccgact gatttacaat gtttgaatca
     7801 agcttttttt cttttcttgg ttgttgcact attgttgcac ttttttattt cagtgccttg
     7861 taatgtggaa ccttcactcg ggatcgaact gatgacctca tccttaccat ggaaaggttc
     7921 agaatttgat gcaagctaca caagacttga tattgctgaa ctatagaatt ttcaatatct
     7981 atgatcttat aaaaagcaaa ctaaatagaa gtaatccttt ccatagcttc aatatcattc
     8041 tcagtaaatg tccatatagc ttcaacaaat ggggcagtaa ttgcaccgga tatacaccgg
     8101 atatcaggtt gattttattc ttgcatattt ccctggatag aagtaaaata cctttatcat
     8161 ataaaaggct ttgaacattt gaaaatttaa tactatacat tgtaaaacct cctgaattat
     8221 taattttttt catggtaaca tgggtgccaa aatggtgccg aactttataa agttacctgt
     8281 taaaaagaaa taagaagaaa aaaggcaata taaaaaaacc tgatttttgt gcatatttca
     8341 acaggtaagc tgtaagaaca cacaaagctt tataagcaaa tatttcaaat cacatatctg
     8401 gaaataatat attttaaatt tcattattga caaatcaaat ttataggtgt attattgtgt
     8461 tactgtacta agaacttaat acagtaacac aataatacaa caacccaaat atataaataa
     8521 acaaaatagg taatatttgt ttataaagta aaatctattt ttaagtataa acaaatacat
     8581 ttattttgat aaggaggttt aaaatttggt atggcaattc aaggctgata ggccaattta
     8641 cactcagtta gtagagcaaa tagagcttag gattgtttca ggggagtata gtccgggcag
     8701 caaacttccg tctgtacgag atttggcggc tgaggcatca gtaaacccaa atacaatgca
     8761 aaaagctctt gcagagctgg agcagaatgg acttctttac tctcagcgaa ctagcggcag
     8821 gtatgttacg gaggatgaaa atatgatacg aaaagtgaaa gatacaattg cactagaaga
     8881 aataaaagat tttttgcaaa aaatgagcaa gctgggattc aattcccagg aaacaatctc
     8941 tttagtagaa agaataataa aggagatgat accacaatga atccaatctt agaatgtaga
     9001 gctcttacaa aaaaatatgg aaatacacaa gcactttcag atgtaaattt aacaatcgaa
     9061 agaggaagaa ttatagggct tttaggaccc aacggaagtg gtaagactac ccttatcaag
     9121 cttgcaaacg ggcttttgac gccaaccggg ggagaaattc ttattgatgg aaagcatccg
     9181 ggtattgaaa caaaaaaaat tgtgtcatat cttcctgaac gtacttactt aaacaattgg
     9241 atgtgtgtca gggaagttat agactttttt aaggactttt acagcaattt taatccggag
     9301 aaaacctatg aaatgttaaa gcggcttgcc attaatcctg atgataagtt gaaaacattg
     9361 tcaaagggta caaaggaaaa agtgcagctc attttggtta tgagccgtga agcagacatt
     9421 taccttctgg atgagcctat tggcggtgtt gaccctgcag caagggatta tattctaaat
     9481 accataatca gtaattataa cgaaaatgct gcggtaatca tatctacgca tttgattgcg
     9541 gatatagagg cggtacttga tgatgtagtt tttcttgcca atggaacaat agctcttgca
     9601 gaaccggttg ataaaatcag ggaagaacag gggaaatcaa tagatgggtt atttagggag
     9661 gttttcaaat gctaggaaaa ttatttaagt atgaaataaa ggcatctgca aggatatttt
     9721 tgccattata tgcttcttta atcctatttg ccattattaa taacattttt ataagaatta
     9781 ataatagcag taacaacaac cagttgttta tgaacatcat gcaaggaatt agtacatttg
     9841 cttatgtatt tttaataatt tctatttttg tattcacatt atttgtaatg attcagcggt
     9901 tctataaaaa tttgcttggt gacgagggtt atttgatgtt tactctgcca gtaagaacag
     9961 gagaacacat tgcagcgaaa ttgttggcct ccatgatgtg ggtaatcacc agtgttatta
    10021 tgactttatt ttctgttatg cttattgcat ataatgaaaa gttttttgca tcttttcaag
    10081 aagcaataag tcaaatactt acagagatta accgtgtatt tggtgtcaac ggatatgcat
    10141 atatagttga atttgcagtt tttatattaa tggttttagc aacaaatata cttatgattt
    10201 atgcttctat atcagtgggg caaatgataa gcaaacacaa gctgctgggt tcgtttggcg
    10261 catatatggg attttatttg atttcgcagt ttattatttc aattatttta gtaatcggat
    10321 attctgccgg atcacgacat tggacttcta ttgagaatat aatgcaaaac aatactacaa
    10381 tacctcattt cttatttgca ggtataatgc ttttgaattt ggtctttgca gctgcttatt
    10441 atactattac aaatttgatt ctttcaagac gtctgaattt ggagtaaatt tgaaatgtgt
    10501 tgcggggcgg gagactggcc acaagcatag cttgcgacca gcccaagcat cccggatatg
    10561 ctcaccgcta tagtaactgt atacagcagc cttgcatttt agcaagcaat gctatgcttt
    10621 ggaacagcaa ggctgcttat agcggttatt ttttgagatt tttctgtacc tctcaagatt
    10681 attggggtta tctgaagata attatgttta tttagttact ccaaagcaaa tttgcttgtt
    10741 taggtcactc caaaataaat ttgctgttta atttatcaaa agttaacttg cttattttgg
    10801 tttatggctt ttgatggaca tttttctatt gcttcattca ctattttcat ttccccctgc
    10861 tttatatcct gctttatatc ttgttttata cattgcttaa taacctttgc ttttttgttt
    10921 tctactttaa aatactccgg cgctattttt gcacaaaggc cacaaccaat acaatattct
    10981 gaggaaatgt tgagtgtatc tacactagaa ttttctttta tgtcatccaa tgggagatct
    11041 tcttttttta ccaacttaga tgtaacataa cccattataa aaatggcgat tgtagtaagt
    11101 atccatcgta caatcataaa tttaggaccc aggaatttag cttcgtttag aagcataggc
    11161 acttttataa ccgcccatgt actgagtagg attacaatat tagagatact tgctcctttt
    11221 gaaagaagcg ctttacacac gggaaatgct gcatatatcg ggcctgctga aaaactgcca
    11281 agtaaaaagg aaaaaataga accttttatg ccagaatcct tacccaaatt tttctcgatt
    11341 gctttttttg gaacccaggc tgttataagt gcggtcagca gtaatattac cggcataatc
    11401 ataagcatct ctttaatata atataagctg ttcttaagtg attgcactgc cttatcaggc
    11461 atgaagatcg ataataaaat atacgaaaga gcaacgagga tcaaaagctt gtttcttctg
    11521 gaaaattgta tgactttgct cataatatca cccccataat caaagcaatt gatatagcaa
    11581 atataaagct taaagagttt ctaattaaag tgaatttcgt accaaattct tttttctcaa
    11641 ggggaaacgt aactatccca accatcgtta atgtggttaa gaatgctacc acaggaacaa
    11701 tgcttgcccc ggcatttacc agggagccgg caagaggaaa agcaacaaaa gctggtatta
    11761 aggtgatgca gcctacaagt gcaaaaacaa ttgttgtaac tatggaattt gattgtcctg
    11821 catactgctt tattgtttca ggaggaagaa aggttagaat cagacctatt aaaaatagaa
    11881 tagctactat ttctcctgct atatgcttca tcattccact tgacatttta acggaatcaa
    11941 tggttttttt cttatcttta ataattgaac cgataaacca aattagtgta ataatccaaa
    12001 aggctaaagt gaaaacatcc atattattaa ttacctccat aattcttagt attaggtgat
    12061 tttactacat gactgctaat actgcttcat aatcaattag cttttcaaat gtttctgcat
    12121 ctgttattga aaaactataa ctttctctaa cataaaaacc tccaaacccc cttttaatat
    12181 gtaattttct atagtatact ttaaaacagg cataaaaaga gttaccatgg taactaattg
    12241 gggtggtaaa tataaaaatc gaaaattata ttgatgccat attaagtacg aatcttttta
    12301 gcggcttttc aaaagatgat ttaattcaat tgtttaattc atctaattat agggtgggaa
    12361 aatacgacaa aggacaggtt cttcatatac aaaatgaact atgtgaatcg atggacatga
    12421 tattagaagg taaactatct gttcaaaaaa tagacgagga agggaatgta ttaaaagtgg
    12481 ctgttttttc agatggggac atattaggag caaacctttt gttttcaaac agcaactcat
    12541 atcctatgac aattgtttcc gaatcaaaaa cagctatact tcatatttat aaggaattaa
    12601 ttctggagct aagccaaaat aacgttactt ttacatctaa cctattgaca ataatatcta
    12661 ataaaacact tgtgctaaca gataaaatcg atgcaatatc tcttaaaact attcgacaac
    12721 ggatcaatga ttttttgaaa tatgaatatc atattcagaa aagcaacgtt attaaactaa
    12781 atatatccaa gaaggattta gccgaaagat taggtataca gagaacttcc ctcagcaggg
    12841 aattgaataa aatgcggaaa gacagactaa tcgatttcga ctcaaaaact attacagtca
    12901 aggatgctaa atacttagta taaaatctgc gagactgtaa ccaacgtttc cataggaatt
    12961 tcccgtatta ctgaatgaga tactgattat aatggagcgg ttaagcgggc ggatattgca
    13021 ctttacaggt ctaaagacca aggggaaaac caggtgaaca tagaacagaa atacaaaaaa
    13081 caataaactt gtggcggttg aataaatata ttgaataagc atttgttatg tgatatcgtg
    13141 taagtgagtt aagtgagtta agtgagttaa gtgagcttgt gcgaggtgat atttatttag
    13201 ggggttatct gaaattgagt tttaatgatt taattaatag aattccagcc aatattcatg
    13261 ctcgtactaa aactttaata agcggagata ttgctgtctt caaaccagaa aaatatgtta
    13321 ttgaatcaaa gatacgaatg aatgattatc attttataat atttcattca actcctccca
    13381 tggctaagat tggagacagg gaatttcagt ttaaaaaggg aagtcttgtt tctctggagc
    13441 caggaactga aattactgtg tatccccatg agaaaaatca acagtgtcaa tatatgtcaa
    13501 ttagtgcaaa aaagaatttt gtggagagga ttgcatttga agcttttggc agagataaaa
    13561 taaaatttga caagcttgag aatacatata gcaagcagct tctcgagtcc atcaacaact
    13621 ttgagtttga actaactaac tttggggata aatacccgat aatgcttcag agtattgcaa
    13681 cccagatagc ttttctatta ctaagagata catttgcagg gtcacagaga tatagtgaaa
    13741 tgagcattac gaacgatgat gattatgtag ctaaggcaat agattatatg aaaaaatatt
    13801 acagttgcaa tattacaatt gacgggatat gtcgagcaat atatcttagc cccagttatt
    13861 ttagcaggaa gtttaaaaat aaaaccggta tgacaccata taaatttcta ttagagttac
    13921 gattggcgaa agccaaagaa cttctttcag agggtgactt ctctattgag gaggcatcaa
    13981 ggctgtgcgg ctttgcgaat tcggcgcatt tatctacagc ttttaagaaa agtgaaggat
    14041 tgtctccatc cgaatataga aagcaattcc aatatattca tatccatgat atgaaaaatc
    14101 agcagcgcta agtttattta taacttatcg gaaagtataa atttccttaa tgtgcacttt
    14161 ccgttaaatg agtttcaata aagtttacct ttaaataaag gttgtttttt gaagataaat
    14221 aggtagtatt atagaaatat tctcctccat tatgtgttaa aatttagccg ttaaggtgtt
    14281 ttcaaaagat tcattatcca gtatttccag ttaagttaat aaggcattaa atcaactttt
    14341 ttgaaaaccc aatctactag tttatttttt attataccta aactatatat agaggagagg
    14401 attaatttgt tcaaaaaagt agtaccagct ataatttgcc tggcattgtt tctaacaaac
    14461 accttgttta ctatggctga acatcagaat attcctgcac cggaaaacct gactgcagag
    14521 attttaaatt atagtgatgg aacaccctat ttcaaaataa ccttcgacac cccttcatct
    14581 cttaaagctt tagcgggtaa agagtatgaa tataatgaag caggtggaaa ttactttatc
    14641 gaattggaat ttaaaattgg ggatggcagt tggtattcgg gagggagcgg tttttcttat
    14701 gaagcgtggc aaaatgtaaa cttagggcct gaagatgtgg gagtagatgg aaatattgat
    14761 ataaaagcaa acacatacca ta
//
LOCUS       DAOSDD010000098        14743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1621_length_14743_cov_4.893859, whole genome shotgun sequence.
ACCESSION   DAOSDD010000098 DAOSDD010000000
VERSION     DAOSDD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_14743_cov_4.893859"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK733_10040"
     CDS             <1..629
                     /locus_tag="PK733_10040"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ10917.1"
                     /translation="VVENYKPTIKIDPSWEMVKLGDVCTILNGYAFRSSMYVDEGIQI
                     VKMGNVQRGKLELDKNISYYPKDLEEEFEKYLLKYGDILISMTGTQGKEDFGNVCMVD
                     AHNKLFLNQRVGKFEVNNSNICREYLYYILQMDIFRKQVFKNASGVRQGNISKIDLES
                     FSIPLPILATQQQIVVKINEEMAIVDQNKRLIGIFEQKIKDKISEVWG"
     gene            734..1765
                     /locus_tag="PK733_10045"
     CDS             734..1765
                     /locus_tag="PK733_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10918.1"
                     /translation="MSYIVKSTERLRKPGAEMETKALLYLMNFREDSDKIHYFVVDFF
                     NDLTGMDRASSKLWDMQSKGASNSSPNSIGRELVTLYKNYLSDFTFSYLILFLGGVSN
                     TVRIDNSKNVFGIENIRPQALIKVIEGLKEESDTKTYIDSSKITTTSINNFLKDVIFV
                     IDDKSPCEYVKAIIKDHPCIIPEEKVLNAIFNEIRDEQSGKKNSHVIEGLVISTSDEA
                     LNYYRHLTNNEIRLMTLQRIINRDPVAQGLPLSFMPILNQCPPERQKDMIDECKGSLC
                     RALFNKNTAQSFWSIFEQIYFLVLNNPTDDVQHLYRKVDSTLIYNCPDFDTLSVKYFI
                     SVVKDGIQT"
     gene            1762..3372
                     /locus_tag="PK733_10050"
     CDS             1762..3372
                     /locus_tag="PK733_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10919.1"
                     /translation="MIIKKIAVGNAEESFVEDSLSEGFNIISSDDNNKGKTIIIQSLM
                     YALGNEPAFPKSFSYKKYFHYVEFEVDEITYYICRRNDGFVLKTPSALMMFDNVSELK
                     RYWTKNIFVLPEIYKNEMLRIVDPVLFLQLFFIGQDKKTTNNISNAGYYNKIDFINML
                     FNFCGLGFPHLSSEDVLITKKQISQLKEERDNLLKQHKILKSSKTAVNYLSAISDKTS
                     FDEKIKQLNKINDKIADLRKERNIAATRKARWETTLKELRSLNRTIDCGELRCMECDS
                     TNISFSRAKKSSYAFDVSNVDMRNEIIGSINEKIASYNEEIEKVASLINKEQEYLQEL
                     MSDDNISLESIVAYKNQIFGSSDAELKIKEIDSQLEILNNSLHSNEISTKKEKEQQDK
                     LIDAIVSLMIAAYTTIDSNSNSIIDGIFTKRDELFSGSEATVFHLAKLYSIQKNTDHN
                     FPLVVDSFRAEDLSTPKEKIVLDLYKELKNQKIFTTTLKTEELGKYDNLSYINSIDYK
                     EHVPSKMLNDLHNTEFKKLLSNILIELN"
     gene            3663..4052
                     /locus_tag="PK733_10055"
     CDS             3663..4052
                     /locus_tag="PK733_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10920.1"
                     /translation="MKNNQPIRLFPTTTISIPVDNVVTELLRTMFQSKLGSIELKIDK
                     TDKTGSQLVISCFLSNEASQHFNSLTQMYAVSEKTIDYADLKEIIIQILNDYLSLGDD
                     DTLDNYNEEDDLEDWDDEDWDDDELRF"
     gene            4377..4913
                     /locus_tag="PK733_10060"
     CDS             4377..4913
                     /locus_tag="PK733_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ10921.1"
                     /translation="MDFVYFIENTTGDFNDNSGVFNLIQDLNIDDNEVYTDSGTDKQE
                     LEKLLNALDAGDRLIIRSVVDLSETAKGLLDILSDLQDREIQLCSVSEVFLNGTDYYT
                     AMKGFLEIHWVYREKKRLNAYTLAKEQGKVGRPPNPNIDKAIKLYNLKFSLREIEDLT
                     GVPSSTLYRALKDSKKKE"
     gene            5024..5596
                     /locus_tag="PK733_10065"
     CDS             5024..5596
                     /locus_tag="PK733_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ10922.1"
                     /translation="MIAGYIRVSSRGQADNNSFEEQEKVIKSKYPDAKIYKEVFTATK
                     ATEDRQIFNSLIEKLEPNSILVVSKLDRFCRNTIEGLDVINLLLKRKVSIHILNMGMI
                     DDSPMGKLIVTVLLAFAEFERNMIVERTTRGKEIARQDKNFREGRPKKFTEKQLTLAL
                     SLLKNHTYKQVEEMTGISKSTLVRTKRKEA"
     gene            5859..6032
                     /locus_tag="PK733_10070"
     CDS             5859..6032
                     /locus_tag="PK733_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10923.1"
                     /translation="MIKNKENVDKNKNGTVTSVGKIEVTEVNTPSSQALKNFSVKLIE
                     IKKKLQGEVKVSA"
     gene            6025..7569
                     /locus_tag="PK733_10075"
     CDS             6025..7569
                     /locus_tag="PK733_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ10924.1"
                     /translation="MHDEIIIEDVAIYLRKSRGETEKDLDKHSDALIEICSDKGWKYE
                     IYKELVSGDTIEMRPIIQALLEDVEDDLYDAVMVIDQDRLSRGGTADAEFIKETLVNS
                     KTYLIESSKVFDLSTEQDEFVYDIKSFIARQEYKLITKRLRRGKIQGAKKGLWTNGPP
                     PLPYVYNKEKKILEVDEEKLVTYREIIDSVVKYKKPTNQIAYELNRKGLKTNGKRGKQ
                     LWSSKTVRDTLLDLTHLEYEDGKGKGHIVMNKTKGNGHKKKPAKAPNCEKIDESEWKK
                     YKGLHESLKTKAEHEQIELFLGRKTKAPRKTTAKQIYPLTELLKCSICGHFLGFTERK
                     DRNGLLSVKKCWYTDPYGKKCNNRSGSMNSVIEKINIAIQGHIKGIEEEIEQIDMDKL
                     NSIAEKIQINKKKVKSKEEEIDRHVEAFGAGAYTIDFYTKLLSRLNDEKKALSEDIRL
                     LEIEYKYMQEQSSTDRVNILKEFIEIIKNPNLTWEDQNQLYKTIIDHIDYTRTDDDIQ
                     IQIIYK"
     gene            7656..9944
                     /locus_tag="PK733_10080"
                     /pseudo
     CDS             7656..9944
                     /locus_tag="PK733_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306815.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(9988..10434)
                     /locus_tag="PK733_10085"
     CDS             complement(9988..10434)
                     /locus_tag="PK733_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOJ10925.1"
                     /translation="MVMKEIILPFLGSLAPAILFNTSRKKLIWCGFSGVAGWVAYKLL
                     LRITGDVVLSIFAGSIAIGLYSETMARILKTPATVYTLPGIYPIVPGVPAYYTIEFLV
                     KHDLINAASTGLETIASAGAIAFGIMLMSAVFRLFSKATHGDSSPE"
     gene            complement(10434..11225)
                     /locus_tag="PK733_10090"
     CDS             complement(10434..11225)
                     /locus_tag="PK733_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOJ10926.1"
                     /translation="MIEEINTLAGETHNISIKEVTTIAVKAGEILLTSGAEIYRVEDT
                     INRICKTYKVECESFVLPTGIFLTAYGIDGENTTLVKRIRDRTIDLERIELVNSFSRN
                     LSNNIMDYDSAMNLLKKIEETKGYPFVLKLMAASLTAFVFVFIFQGNVFEGIAAAIIG
                     SIIYFFKEKVLQVGLFPFLGFFISGIIGGGMSVASATLFKGLNVYKIIIGAIIILLPG
                     MAITNGIKDALYGDITSSLNRLSEGIFIVTAVGAGVALILFLLLR"
     gene            complement(11250..12113)
                     /locus_tag="PK733_10095"
     CDS             complement(11250..12113)
                     /locus_tag="PK733_10095"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ10927.1"
                     /translation="MNSFQSSQKNIISGCNNNNRNDTNNGNLESTNRCNPGAIKLEVK
                     NVKKSFDKLPVIDEVSLYMREKEFVSILGLSGSGKSTIFNIISGLLIPDEGEIIIDGK
                     NYTGKIGRVSYMYQKDLLLQWKKIIDNVALPLVIKGEGKKTAREKVRDYFKEFGLEGF
                     EYMYPSQLSGGMRQRTAFLRTYMFSRDIMLLDEPFGGLDAITRSKIHYWIMDVLDKLD
                     TAVMFITHDIEEAVFLSDRIYILSDKPAKVMEEVIIDLPRPRNKEIITTGRFNDFKRH
                     ILKIMDSISRN"
     gene            complement(12094..13152)
                     /locus_tag="PK733_10100"
     CDS             complement(12094..13152)
                     /locus_tag="PK733_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ10928.1"
                     /translation="MQKAKSNLNFKKIMSSIISVLIIMASISLLVTGCNKENVEKKGK
                     DETKDIKDMKEVKVLLDWVPNTNHTGLYVAVEKGFYKEEGLNVTIIQPPEGGGPELIA
                     AEQGQFGISYQEQITYARTTKNPLPVKAIAAIIQHNTSGFASPLEKNIKTPKDFEGKK
                     YGGWGSPMEEAMLKGIMEKNGADFSKLQIVNIGTADFFTSVQRDVDFSWIYYGWDGVA
                     AKIKDFSINFIKLQDVDPILDFYTPVIITNENILKNDPDMVEKFMKATSRGYEYAIEN
                     PEDAVKLLLKHAPEIDEKLAIESQKYLAGEYKSDAKRWGEMKLEIWERYGNWMYEKGL
                     IESKLSAEEAFTNEFLPK"
     gene            complement(13344..14108)
                     /locus_tag="PK733_10105"
     CDS             complement(13344..14108)
                     /locus_tag="PK733_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ10929.1"
                     /translation="MKKLQNIGNRIIPVAFTAVIVLIWQLVSDNAGIPKFVLPSPADI
                     GSAIFKILPELKMHTMTTIWEALSGLAISIGLSIVLAVVMDSILVVKKALYPVLIISQ
                     TIPIIALAPLFIIWFGFGMLPKILVVVLVCFFPIVISLTDGLASVDTDMLNLMKSMGA
                     GKIQIFRHLKLPAACMSFFSGLRIAVTYSIMGAVIGEWLGGEKGLGVYMIRVKHSYSL
                     DKFFAVIVVIVILSIILFKITALIEYALMPWNRAQK"
     gene            complement(14080..14370)
                     /locus_tag="PK733_10110"
     CDS             complement(14080..14370)
                     /locus_tag="PK733_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOJ10930.1"
                     /translation="MAICNVSLQVIPKIRSDKTYEVVDKVIAYIASCNIRYEVGPMET
                     TMEGELDELLDIVKKAQYICIDEGADRVLSVVKIDFKPEGVTIDEKVAKYRK"
     regulatory      complement(14461..14571)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         5199 a         2425 c         2569 g         4550 t
ORIGIN      
        1 aggttgttga gaattataag ccgactatca aaatagaccc aagctgggaa atggtgaaat
       61 taggtgatgt gtgtacaatc ttaaatggat atgcatttag aagtagtatg tatgttgatg
      121 agggtattca gattgtaaaa atgggtaatg ttcaaagagg gaaattagag cttgataaaa
      181 atatttctta ttatcctaaa gatttagaag aagaatttga aaaatattta ctaaagtatg
      241 gtgatattct tatttcaatg actggcactc aaggtaaaga agactttgga aatgtatgta
      301 tggtagatgc tcataataaa ttatttctaa atcaacgagt aggaaaattt gaagttaata
      361 actcgaatat atgtagagaa tatctatatt atattttaca aatggatatt tttaggaaac
      421 aagtatttaa aaatgcaagt ggagtaaggc aagggaatat tagcaaaatt gatttagaaa
      481 gcttttctat tccgcttccc attcttgcta ctcaacaaca aattgtagta aaaatcaacg
      541 aagaaatggc cattgttgac caaaacaaac ggttaatcgg aatatttgag cagaagataa
      601 aggacaaaat ttctgaggtc tgggggtgag taagtatgaa tgatgaaata aatgcttatt
      661 atatcccata attaaacagc catttgttgg caagatcatt gtgactataa acaaaaatga
      721 tgaggtgatg taattgtcat acatagttaa atctacagaa aggttacgaa aacctggtgc
      781 tgaaatggaa actaaagcac tattatattt gatgaatttt cgtgaagata gcgataaaat
      841 tcactacttt gttgttgatt tttttaatga tttaacaggt atggatagag catcatcaaa
      901 actttgggat atgcagtcta agggtgcttc taattcatct cccaattcta taggaagaga
      961 attggtaaca ctttataaaa actacctaag cgattttact tttagttatc ttattctttt
     1021 tttaggaggg gtttccaata cagtaagaat tgataactct aaaaatgttt ttggaataga
     1081 aaacataaga ccacaagcat taattaaagt aattgaaggt ttgaaagaag aaagtgatac
     1141 taaaacatac atagattcaa gtaaaataac aaccacatct attaataatt ttttaaaaga
     1201 tgtaattttt gttattgatg ataaatcacc ctgtgaatat gttaaagcta ttataaaaga
     1261 tcatccttgc attatacctg aagaaaaagt tcttaatgca atctttaatg aaattagaga
     1321 tgaacaatca ggcaaaaaga atagtcacgt tatagaaggt cttgtcattt ctacaagcga
     1381 tgaggctttg aactattatc ggcatttgac taataatgaa ataaggttga tgactttgca
     1441 aaggattatt aatcgtgacc cagttgccca ggggctgcct ttatcattca tgccgatatt
     1501 aaatcagtgc ccacccgaaa gacaaaaaga tatgattgat gaatgtaaag gttcattatg
     1561 tagagcatta tttaataaaa atacagcgca gagtttttgg tcaatatttg agcaaatata
     1621 ttttttggta ctaaataatc ccactgatga tgtccagcat ttgtatagaa aggtagattc
     1681 aacattaatt tacaattgtc ctgattttga tacattatcg gtaaaatact ttatttcagt
     1741 tgtaaaggat gggattcaaa catgataatt aaaaaaatag ctgttggaaa tgcagaagaa
     1801 tcttttgtag aagattcact gtctgaagga tttaatataa tttcaagtga tgataataat
     1861 aaaggtaaaa ccataatcat acagtcgtta atgtacgctt taggaaacga accagcattc
     1921 ccaaagtcct tctcttataa aaaatatttt cattatgttg aatttgaagt agatgaaatt
     1981 acatattaca tctgtagaag aaatgatggt tttgtgctaa aaactccatc tgctcttatg
     2041 atgtttgata atgtttctga gttaaaacgg tattggacaa aaaacatctt tgttttacca
     2101 gaaatatata aaaacgaaat gttaagaata gtggatcctg ttcttttttt gcaattgttc
     2161 tttatagggc aagataaaaa aactactaac aatatttcta atgctggcta ttacaataag
     2221 attgatttca taaatatgct gtttaatttt tgcggtttgg gcttcccgca tttaagttca
     2281 gaagatgttt tgattacaaa aaaacaaatt tctcaattaa aagaggaacg ggataactta
     2341 ttaaagcaac acaaaatatt aaaatcatct aaaactgccg ttaattattt aagtgcgatt
     2401 agtgacaaaa catcttttga cgaaaagata aaacagttaa ataaaattaa tgataaaata
     2461 gctgatctgc gaaaagaaag aaatattgca gcaactcgaa aagcaagatg ggaaacgaca
     2521 cttaaggagc ttaggtcttt aaatagaact attgattgtg gcgaattacg ttgtatggaa
     2581 tgtgattcaa ccaatatttc tttcagtaga gcaaaaaaat cctcatatgc ttttgatgta
     2641 tctaatgttg atatgagaaa tgaaattatt ggttcaataa atgaaaaaat agcttcttat
     2701 aatgaagaaa ttgaaaaagt agcttcttta atcaacaaag aacaggaata cttacaagaa
     2761 ttgatgtccg atgataatat ttcgcttgaa tcaatagttg catataagaa tcaaatcttt
     2821 ggttcatcag atgctgaact taaaataaag gaaattgact cacagttaga aatattaaat
     2881 aattctctcc attcaaatga aattagcaca aagaaagaaa aagaacaaca ggataagttg
     2941 atagatgcta ttgtttctct tatgatagca gcatatacaa ctattgattc taatagcaat
     3001 tcaattattg atggaatatt taccaagaga gatgaattat tctctggaag tgaagcaact
     3061 gtttttcatc ttgcaaagtt gtattccatc cagaaaaata cagatcacaa ctttccgtta
     3121 gttgttgact cattcagagc tgaagattta tcaacaccaa aagagaaaat agtattggat
     3181 ttatataaag aattaaagaa tcaaaaaatc ttcacaacaa cactaaaaac agaagagctt
     3241 ggcaagtatg ataatttgag ttatataaat agtattgatt acaaagaaca tgtgccaagc
     3301 aagatgttaa acgatttaca caatacggaa tttaaaaagt tactttcaaa tatattaata
     3361 gagctaaact aaaaattgat tcctttatct gtgtgaaaga tagaattaca taaaattcaa
     3421 aaaaagactt atagctacct gcaatgtcaa tagaatatta tcttacaaat cccgtatagt
     3481 tcatgcggga ttttttgtcg taaactttta tgcattgtct tggtcatgaa aacatttttt
     3541 atctaatgca attattcgat gggcaaacaa gaagactttt ggacatagtt ttattcagac
     3601 actgcagtaa atattactat tttttgaata cagaaaaatt caataattgg aggtcattta
     3661 aaatgaaaaa caatcagccg atcagactat tcccgactac cactatatca attccagtag
     3721 ataatgtggt gacagagctt ctgcggacaa tgtttcagtc caaattgggg tcaatagagc
     3781 tcaagattga taaaacagat aaaactggtt cgcagcttgt tatatcttgt ttcctttcga
     3841 atgaggcaag ccaacatttc aatagtctaa cacagatgta tgcagtaagt gagaaaacca
     3901 tagattatgc agaccttaaa gaaattatca tccaaatcct aaacgattat ttatccctgg
     3961 gagatgatga cacattggat aattacaatg aagaagatga tttagaggac tgggacgatg
     4021 aggattggga tgatgatgaa cttagatttt aacagctcaa tcctctaccc tgttccatat
     4081 tttgtccaac agggttgtcc taagtaaaat aagtgcagat tttgagtatc tcaaaatctt
     4141 tagagcttta tggatgccca aagcaaacat attgggaata tctcaattcc attcccaata
     4201 ttattcggta tatttctttt aacagaaatc cgttccgaaa gtcagtactt gaacttcagg
     4261 aaaatagtaa taatattggg aatgaatttt gggataaaat catgatgaat taaaatatta
     4321 gaatttttgg gattgccaaa atctcaaggt attacaggag aggagcagaa aaaattatgg
     4381 attttgtata ttttattgaa aataccacag gagattttaa tgataactca ggggttttta
     4441 acttaatcca agatttaaat attgatgata atgaagttta taccgacagc ggcactgata
     4501 aacaggaact ggaaaagctt cttaatgcat tagatgctgg agatagactg ataatccgtt
     4561 ctgttgtgga cctatctgaa accgctaagg ggcttcttga tattttatct gacttgcagg
     4621 atagagaaat tcagctatgt agtgtatcag aggtatttct aaacggaaca gattactata
     4681 cagccatgaa aggctttctg gaaattcatt gggtataccg tgaaaaaaaa cgtctaaatg
     4741 cgtatacgtt agcaaaagaa cagggtaagg tcggcaggcc ccctaatccc aacattgata
     4801 aagcaattaa gctttacaac cttaaattca gtttgcgaga gattgaggac ctgactggag
     4861 ttccatcctc tacactttac agagccctta aggatagtaa gaaaaaagaa tgatattaga
     4921 ttaaatagcc aaaacttata agttttgatt gtaaaaaaca aaccgcataa aatatagtgt
     4981 tgaatggtgt cataactaat gagcaaaaga gaaggtggta aatatgatag ctggatacat
     5041 aagagttagc tccagaggac aggctgacaa caattcattt gaagaacaag agaaagtgat
     5101 aaaaagcaaa tatcctgatg caaagatata taaagaagtt ttcacagcaa ctaaagctac
     5161 tgaagacaga caaatattta attctctaat tgagaaactt gaaccaaata gtattttggt
     5221 ggttagtaag cttgatagat tttgcagaaa tactatagaa ggattggatg taatcaacct
     5281 tttgttaaaa aggaaagtat ctatccatat attgaacatg ggcatgattg atgatagtcc
     5341 aatgggaaag ctaattgtca cagttctatt ggcttttgca gagtttgaaa gaaatatgat
     5401 agtcgaaaga accactcgcg gaaaagaaat agcaaggcaa gataaaaatt tccgtgaggg
     5461 tagaccgaag aaatttactg aaaagcagct gactcttgct ttgagtttac tgaaaaacca
     5521 tacctataag caggttgaag aaatgactgg aatttctaaa agcacacttg taagaaccaa
     5581 aagaaaagag gcatgaaata ttattctttg agataacact tattacttca aattacatga
     5641 acaaggtgtt taagcaatat aagctgttct gtttataaga atagaagatc cagagagcat
     5701 ccctttgtat tatgagttta tctcattacg aaatgggtgc ttttttattt atgaaaagga
     5761 cataaatgaa aaagttgata gcagttgaac cataaatgcg aactgttttt ataacagatg
     5821 aaagtaatca atgcaaacga aatttagggg ggtttgcatt gataaaaaat aaagaaaacg
     5881 ttgacaagaa caagaacggg acggtaacat ctgtaggaaa aattgaggtt acagaagtaa
     5941 atacgcccag ttcacaagcg ctgaaaaatt tcagtgtaaa attaattgaa atcaaaaaaa
     6001 agctacaagg tgaggtgaaa gtaagtgcat gatgaaataa taattgaaga tgtagctata
     6061 tacttaagaa aatcaagggg tgaaacagaa aaagaccttg ataagcatag tgatgcactt
     6121 atagagatat gcagtgataa gggctggaaa tacgagatat ataaagaatt agtatcaggt
     6181 gataccattg aaatgcgacc tataatacag gcgttgcttg aagatgtgga agatgacttg
     6241 tatgatgctg ttatggttat agatcaagat agactttcaa gaggtggtac cgctgatgct
     6301 gaatttatta aagaaacttt agtaaatagc aaaacatatc taatagagag tagcaaagta
     6361 tttgatttgt ccactgaaca agacgagttt gtctatgata ttaaaagctt tattgcaaga
     6421 caggaataca agttaataac gaaaagatta agaagaggca agatacaggg tgcaaaaaaa
     6481 ggtttatgga caaatggacc acccccactt ccctatgtat ataacaaaga aaagaagata
     6541 ttagaagtgg atgaagagaa gcttgttaca tatagagaga ttatagattc tgttgttaaa
     6601 tataaaaagc ctacaaacca aattgcttat gagttaaata gaaaaggttt gaaaacaaat
     6661 ggaaaaagag gaaagcaatt atggtcaagt aaaacagtaa gagacacgct cttagattta
     6721 acacatcttg agtatgaaga tggcaaagga aaaggtcata tagtaatgaa caagacaaaa
     6781 ggtaatggtc ataagaagaa gccagcgaaa gcacctaact gcgagaaaat agatgaaagt
     6841 gagtggaaaa aatataaagg gttgcacgaa tcattaaaga ccaaagcaga acacgagcaa
     6901 attgagctgt ttttgggtag aaaaacgaaa gcaccaagaa aaacaacagc aaagcagata
     6961 tacccgttaa ccgagttgtt aaagtgtagt atctgtggtc attttcttgg atttacagag
     7021 agaaaggaca gaaacggctt attgtcagta aaaaagtgct ggtatacaga tccttatggt
     7081 aagaaatgca ataatagaag tggttcaatg aactcagtaa tagaaaagat aaatatagct
     7141 attcaagggc atataaaggg tattgaagaa gaaatagaac aaatagatat ggataaatta
     7201 aattctattg ctgaaaaaat acaaataaat aaaaagaaag tcaaaagtaa agaggaagag
     7261 atagatagac atgttgaagc atttggtgct ggtgcttata caattgactt ttatactaag
     7321 cttttatcaa ggttaaacga cgaaaagaaa gcgctttctg aggatattag acttcttgaa
     7381 atagaatata aatatatgca ggagcaaagc agcacagaca gggtaaatat actcaaagaa
     7441 ttcattgaaa taataaaaaa tcctaattta acttgggaag accaaaacca gttgtataag
     7501 actataatcg accatataga ctacacaaga acagatgatg atatacagat tcagataata
     7561 tataaataag ttattaagga tattgctttt ttagtagtat ccttaaattt ttgggtaata
     7621 ctaaaatgaa atactttatg aaggaaggtg ataaaatgac taacaataat gttgtaccca
     7681 atagattgat aaatgaaaaa tcgccatatc tattacagca tgctcacaat ccagttgatt
     7741 ggttcccttg gggccaagag gcgtttgata aagcaaaagc tgaagataag ccgatatttc
     7801 tttctattgg gtactcatag cgttggttat agaaaccaac ttgtcattgg tgtcacgtaa
     7861 tggaaaaaga atcctttgag gatatagaag tcgctcatat cctcaataat cactttatag
     7921 caattaaggt agaccgtgaa gagcggcccg acatcgacca tatttatatg tcagtatgtc
     7981 aaactcttac aggcagtgga ggctggcccc ttacaatctt tatgactcct gatgccaaac
     8041 ctttttatgc gggtacctac ttccccaaaa atgatcgagt aggtataccg gggttaataa
     8101 atattcttca gcgggtaaat gaggcatgga aaaataacag ggatgctctt ttgcattcca
     8161 gtgaagaaat cctgaatgca gttaataagg ccaccaatgc cgaaaatgat gtaaattatg
     8221 taaatactgc aaatgctgta aacgttgcaa ataacgcaaa tggtgtttat actgcatata
     8281 acaagtcatc aggattaatg aaaggtttat atagtgatgc tatacatgaa gccttttctc
     8341 tatataaacg ctcttttgat agtcaatatg gaggattcgg aaggacccct aagtttccca
     8401 cacctcataa cttattattt ctgctcagat actggtataa aacgcaagaa cccaccgctc
     8461 ttgaaatggt tgaaaaaaca ttggattcaa tgtaccgggg tggtatatat gaccatattg
     8521 gatttggttt cagccgctat tccaccgata gaaaatggct tgtgccccat ttcgagaaga
     8581 tgctttatga caatgccctt ttggcaatag catatttgga aacttaccag gccacttata
     8641 ataaaaagta tgcagatatt gcaaaggaaa tatttactta tattttgcgg gatatgacct
     8701 ctcctgaagg tggtttttat tctgctgaag atgctgactc tgaaggagaa gaagggaaat
     8761 tctatgtatg gtctgatgat gaaatcaaaa aagtgctcgg cgaagaagat ggagagaaat
     8821 tctgcgctta ttataatata acagcacagg gtaattttga aggcaaaaac attcccaacc
     8881 tgattacaaa gtcttctggc ataaatacta ttgataccga agtcactggc atcggaattg
     8941 tcaatactgt ggctaaaaat actaagtcaa taaatactga gccaaataat accgagttaa
     9001 ctaatactga gcttattaat actgaatcaa taaatgctga atttattaat aacaagttta
     9061 ttaaagcctg ccgtgaaaag ttgtttaaac ataggaaaaa tagaattcat ccttataaag
     9121 atgataaaat attaacatcc tggaatggtt tgatgattgc cgcaatgtcc attggaggaa
     9181 gagttcttaa agaaaaacaa tatacttctg ctgcagaaaa ggctgttgat tttatattta
     9241 cgaagctcat acgagaagac ggcagattac tggctcgttt ccgtgatggt gatgcttcat
     9301 ttccggcata tgcagatgat tatgcctttt tgatctgggg tttgattgaa ctatatgaaa
     9361 caacattcaa acctgattat ctgcaaaagg ctttatatct taataatgaa cttatagaat
     9421 acttttggga tgaaagaaat ggaggattat ttatttatgg cagcgatagc gaacagttga
     9481 ttattaggcc gaaggaaata tacgatggag caacaccttc cggtaattca gtatcagcat
     9541 taaattttct aaggctgtca aaattaaccg ggcagtatga acttgaggat aagacagagc
     9601 agttgcttaa agcatttagc agtgatataa aaaatcatcc gatcagctat gcattttccc
     9661 ttgtagctgc aatgtttgca caatcatcta caaaagaagt ggtaatcgta tcagatttcc
     9721 acgatactgc aactgatgaa atgcttaata ttatccacca ggggttcaga ccgtttattg
     9781 tttctatgct ctatgcaaac gaatacaaaa gtctgaaatc tgttgtacct tttattattg
     9841 gtcataaatc tattgatggt aaacccaccg cctatatttg tgaaaacttt acctgccagg
     9901 agccggtaac agatattgaa cacttaagag aattgctgaa ttaagggtga tacaggagga
     9961 cagcatagag ggacagtccc cttataccta ttcaggggaa ctgtccccat gtgtagcctt
    10021 tgaaaataac ctgaaaaccg cagacataag catgattcca aatgcgatag cgccggcact
    10081 tgctattgtt tcaaggcctg tactggcagc attgatcagg tcatgtttta caagaaattc
    10141 aatggtataa tatgcaggca ctcccggcac aatagggtat atacccggca atgtataaac
    10201 tgtagcaggt gtttttaaaa tcctggccat agtttcactg tacagcccta ttgcaattga
    10261 acctgcaaaa attgataata caacatcacc tgtaattctt aataacagct tgtatgcaac
    10321 ccacccggct acgcccgaaa atccacacca tataagtttt ttcctgcttg tgttgaaaag
    10381 tattgcagga gccaggcttc ctaaaaaagg aagtattatt tctttcatta ctatcacctc
    10441 agaagcaaaa ataaaataag tgcaactcct gcacctactg cagttacaat aaatataccc
    10501 tcgctcaacc tgttcaggct ggatgtgata tctccgtaaa gagcatcctt aataccattt
    10561 gttatagcca ttcccggcaa taggattata atagctccaa taataatttt ataaacattc
    10621 aatcctttga aaagtgttgc ggatgccaca ctcataccgc caccaataat gcctgagata
    10681 aaaaaaccca aaaacggaaa taatccaacc tgtaaaactt tttccttgaa gaaatatata
    10741 attgagccga taattgctgc agcaatacct tcaaatacat ttccctgaaa tatgaataca
    10801 aatacaaaag cagttaaaga tgcagccatc agttttaaaa caaaaggata ccctttggtt
    10861 tcttctattt ttttcaggag attcattgca ctgtcataat ccattatatt attactaaga
    10921 tttctggaaa aagaattaac caattcaatt ctttcaaggt ctattgtccg atcccttatt
    10981 cgttttacaa gagttgtgtt ttctccatca atcccatagg ctgtaagaaa tatacctgtg
    11041 ggcagtacaa aactctcaca ctccaccttg taggtcttac aaattctgtt tattgtatct
    11101 tctaccctgt atatctctgc gccacttgtt aaaagtattt caccagcttt tacagctatg
    11161 gtagttactt cttttatact tatattatgt gtttctccgg ccagtgtatt aatttcctca
    11221 atcaatttat ctcctccagg gttgctaaat taatttctgc ttattgaatc cattatcttc
    11281 aaaatatgtc ttttaaaatc attaaacctg cctgttgtta ttatttcctt gtttcttggc
    11341 ctggggaggt ctattatcac ctcttccatt acctttgcag gtttatcaga tagaatatat
    11401 attctatctg ataaaaaaac tgcttcctct atgtcatgag taataaacat aacagctgta
    11461 tcaagcttat caagcacatc cataatccaa taatgtattt ttgacctggt tatagcatcc
    11521 aggccgccaa aaggctcatc aagaagcata atatcccggg aaaacatata tgtccttaaa
    11581 aaggctgtcc tctgccgcat cccccctgaa agctgtgatg gatacatata ctcaaaccct
    11641 tccagcccaa attccttaaa ataatctcta accttttctc tggcggtctt ttttccttct
    11701 ccttttataa caaggggtaa agctacatta tctattatct tcttccattg cagaagcaga
    11761 tctttttgat acatgtagct gactctgcct atttttccag tataattttt cccatctatt
    11821 attatttcac cttcatcagg aataagcaag cctgaaatta tgttaaatat tgtacttttg
    11881 ccgctgccac tcaatcctaa aatagacaca aattcctttt ccctcatata aagtgatacc
    11941 tcatctatga ccggcagttt gtcaaaggac ttttttacat ttttaacctc taatttaata
    12001 gctcctggat tgcatctatt agtgctctct aaattaccat tgttggtatc attcctatta
    12061 ttgttattgc aaccactaat aatatttttc tggctacttt ggaaggaatt cattggtaaa
    12121 cgcctcttct gcacttaatt tgctctcaat caaccctttt tcatacatcc agttgccata
    12181 tctctcccat atttccagtt tcatctctcc ccatctcttt gcatcagatt tatactcacc
    12241 tgcaagatac ttctgacttt cgatagcaag tttttcatca atttcaggtg catgtttcaa
    12301 taggagcttc acagcatctt ccgggttctc tatagcatat tcgtaacctc ttgaggttgc
    12361 tttcataaat ttttcaacca tatcagggtc atttttaagt atgttttcgt ttgttataat
    12421 aacaggagtg taaaaatcaa gtataggatc tacgtcctgt agtttaataa aattgataga
    12481 aaaatctttt attttagccg ctacaccatc ccagccataa tatatccagg agaaatctac
    12541 atccctctgc acacttgtaa agaagtctgc agtacctata ttaactattt gaagtttact
    12601 gaaatcagct ccgtttttct ccataatacc ttttaacata gcttcttcca ttggcgaacc
    12661 ccaaccacca tattttttgc cttcaaagtc ctttggtgtc ttaatattct tttccaaagg
    12721 agaagcgaag cctgaagtgt tatgctgtat gatagctgca atagctttga ccggcaatgg
    12781 gttttttgtg gttcttgcgt atgttatttg ttcctggtag cttatcccaa attgtccctg
    12841 ttcagcagct atgagttccg ggcctccacc ttcaggaggt tgtattatgg ttacgtttaa
    12901 accttcttct ttgtaaaatc ctttttcaac agcaacatac aaaccagtat gattggtatt
    12961 gggaacccag tcaagaagaa cctttacttc tttcatgtct tttatgtctt ttgtttcatc
    13021 ttttcctttt ttctctacgt tttctttgtt acatccggtc actaataatg atatactagc
    13081 cattataatt aaaacactaa taatacttga cattattttc ttaaaattta aatttgattt
    13141 tgctttttgc atattatttc ctccatgttc attctatatt tgcttacata tgctaatact
    13201 actttgttat attgacaatg tgaagcgcat ttcccctcac atcaccttcc cgatgttgta
    13261 aaaattatcc aggaagacat cgctttccaa attgcatagt agtgctaaag tgtatattta
    13321 gctttctgcg taatgcatat ccattatttt tgagctctat tccatggcat taatgcatat
    13381 tcaataagtg ctgttatctt aaatagtatt atgctcagaa taacaattac aactatgaca
    13441 gcgaaaaatt tatcaagtga gtatgagtgt ttcactctta tcatgtatac acccaaaccc
    13501 ttttccccac caagccattc accgattact gctcccataa tgctgtaagt aactgctatt
    13561 cttagtcctg aaaaaaagct catacaagca gcaggaagtt tcaaatgcct gaatatttgt
    13621 attttacctg cgcccataga tttcataagg tttaacatat ctgtatcaac agaagccaaa
    13681 ccatcagtaa ggcttattac aatagggaaa aagcacacca ggacaactac aagaatttta
    13741 ggcagcatac caaatccaaa ccaaattata aataacggtg caagagctat tataggtata
    13801 gtttgcgaaa ttattaaaac aggatatagt gcttttttaa caactaatat actatccata
    13861 acaactgcca acactataga taaccctatg gatatcgcca gtcccgaaag agcttcccaa
    13921 atggttgtca ttgtatgcat tttcagctca ggaagtattt tgaatatagc tgacccaata
    13981 tctgcagggg atggaagtac aaacttgggt ataccagcat tgtcactaac caactgccat
    14041 attaatacta ttacagctgt aaaagccact ggaataattc tatttcctat attttgcaac
    14101 tttttcatca atggtcacac cttcaggttt aaaatctatt ttaacaaccg aaaggactct
    14161 gtcagctcct tcgtcaatac aaatatattg agccttttta actatatcca aaagttcgtc
    14221 cagttctccc tccattgttg tttccatagg tccaacctca taccttatat tacacgatgc
    14281 tatataagct atcaccttat ccactacttc atatgttttg tcacttctta ttttaggaat
    14341 aacttgtaag cttacattgc aaattgccat atgagcacct ccttaaagta ttttaaattt
    14401 ttgcctttaa ataaaataag ccctccatcc tgaacagaat aaagggttta tgtctgtata
    14461 caaaaataca aatattctcc ctccgctggc attatccagt tcaggttaaa ttgggtcggg
    14521 acaacacagc cccctctcag ccgatttaat tcagctcccc agataacgaa gttctatttt
    14581 atatatctat aataatataa ttaattggca aagtcaacgg ggcatatttt cttcttttga
    14641 tgcaaaatat aaaaaatgct cccaaattat cagaacaaat taatgaaact ccctaattct
    14701 gcagtaaaaa tttaacaaat cattaatcaa acccttgata caa
//
LOCUS       DAOSDD010000099        14658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1631_length_14658_cov_5.115045, whole genome shotgun sequence.
ACCESSION   DAOSDD010000099 DAOSDD010000000
VERSION     DAOSDD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1631_length_14658_cov_5.115045"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..701
                     /locus_tag="PK733_10115"
     CDS             177..701
                     /locus_tag="PK733_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ10931.1"
                     /translation="MRRPKVQFITRTAILLALTIMFQMMGRFLGPNNNFIVGPLVNAA
                     LLIATAATGLWGATTISVIAPFVSALTNKAPIAPILLPFTPFIAIGNFILVLFFYLFM
                     KKNKIAGIITGSVLKFSFLYASISVFLKVMKVPEGPSKVLNSLFSWPQLVTALAGGVI
                     ALVVIKALGKNIEM"
     gene            complement(613..2127)
                     /locus_tag="PK733_10120"
     CDS             complement(613..2127)
                     /locus_tag="PK733_10120"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ10932.1"
                     /translation="MDKRQSINKKKIANKLKYIFLGLAIIILVTGVFGRDFIVNLFTK
                     LQMDELVSPVEGTRLLVVAPHCDDETLGCGNLISKTIESGGTVKVVLLTNGDGHTSAV
                     ALNYLTVKPNAKNYISSAYLRQDETERAMKRAGVDRENIIYLGYPDGGLYHMWGENWD
                     ENNLYVSRFTKCSHSPYSNSYKSDAPYCGKSIADDLESIVMTYNPSIIAFPHPNDRHP
                     DHFAAYCLVRYVLAKNNMTPDQYLYLVHRGKWPSPFGQFSKLFLVPPKKLVDIGTKWV
                     SSPMSDIEQKQKADMLSEYKSQMNVNSPFLKAFIRQNELFGIYPDLEYDFSMRDGPKI
                     LLLEDPVSDKIKQNASKSADIKNVYLSTDSSNMHLFLDTVESARRNINYYIDMYFFSS
                     DGKTFRINMTVNYLKTIDLQTDTSENIASIDGLRLMPDSEMLHIKIPYSGIPDFDSIL
                     IGASTSSASNGIDRTAWRLVKWDRGSTSLYSSQGPLLLPMQLLPLPKLLPAAAS"
     gene            complement(2233..2895)
                     /locus_tag="PK733_10125"
     CDS             complement(2233..2895)
                     /locus_tag="PK733_10125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ10933.1"
                     /translation="MPEILIRKYNDTDLEQVLNICHKTGYMGKDLEGTGEFGDRKLFG
                     YFFCAYYLLYEKEHCFVAVDSEHDNMVVGYIIGTMNTKRQIIMFMMKMSWRIGLRLLY
                     TFCMYPSILKSIRNMLKNKTYKYMPKNLYNEYPAHLHINMLPEYQGRSIGTSLLATFE
                     NHIKNNPGIQEKGIHLKTSNKNCSAIKFYIKNGYTKLLEFSDTLWGNVEDYKTIIFVK
                     KL"
     gene            complement(3103..4071)
                     /locus_tag="PK733_10130"
     CDS             complement(3103..4071)
                     /locus_tag="PK733_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10934.1"
                     /translation="MLPLQLAGLALAGRALLTKYNNYKPDTQREPGSGTQNQASKNMV
                     IPMAIANSLESLSKTLTSSFSQLKESASKLNRKTESYYTIAKKFVPENTKLLLPESPS
                     YTREIHLSDIDGDLSEELITSFKLNDEIRTVILKKQNDSWYTAAEITNNEYEILDHRS
                     VADLTGAGKKQLILGLSSRGKPSQVYGYSLENGKTNELFSRNYDRLDVLRESKNRAAQ
                     SKAELGVWTRQNDGTYNIEVLGWNGLRLEPVENITTYYNEKAIPYFVQRIRKEPYTVC
                     NWCNLADALVKAGTKRDALTAIDTGMALDRNSKFKNKFTELKNKIK"
     gene            complement(4225..5319)
                     /locus_tag="PK733_10135"
     CDS             complement(4225..5319)
                     /locus_tag="PK733_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ10935.1"
                     /translation="MQAITGIERMSNILKRKPVDHIGLYEHFWSDTYKKWTSDGYIKE
                     SEGFADHFDFDLDECWAFNMTANLDFKPETIEETEETILVRDGNGALLRRHKLHDTTP
                     EHVDFLVKERVDWEEHIKPLLKPERRRINFETYRKAKKYAAEKNRFFVWSGVNVFELM
                     HPVCGHEYMLIGMALDPDWIIDMVDTYSRLTMDLQEILFAEEGYPDGIWYYEDMGFKG
                     KPFFSPAMYKEIIQPGHKRTFDFAHAHDLPVIVHSCGMVESLVPGLIEAGMDCLQVIE
                     VKAGMDLLKLYREYGDILSFMGGIDVRVLYTNDKELIKKELESKIPVAMGNYGYALHS
                     DHSIPNTVDYDTYKYFIEKGLELGSYKSNC"
     gene            5648..6325
                     /locus_tag="PK733_10140"
     CDS             5648..6325
                     /locus_tag="PK733_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent methionine synthase"
                     /protein_id="HOJ10936.1"
                     /translation="MDFMLLDNIKVEISNEELFKSLKIDMEDDIAPEVLGLFEEAWKT
                     ARPKAVYGAAYIEDRGRNWVVINGIRFTSRILSVNLKDAHRVFPYVVTCGRELYEWAM
                     SINGPLERYWADTICEIFLRKAFKTMSEDFIKKFNPGKTATMNPGSLEDWPISEQKPL
                     FSLIGDDNIKTAGIELTESFLMIPVKSMSGIKFLTEYKYENCMLCPRERCPGRRAEYD
                     SLLKEKL"
     gene            6538..7047
                     /locus_tag="PK733_10145"
     CDS             6538..7047
                     /locus_tag="PK733_10145"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HOJ10937.1"
                     /translation="MDVKKQLEEKISDMEKVARIFEIEPFDIYFLGGSACILGGYTDR
                     ATRDFDFIDLNYPSRLGKVFVQLRDFDMLEYESTIISPKYKERAIKLNEFKYLNIYLL
                     SVEDIIVSKIIRLEQKDLQDIYELIKVADKELINKIIDEVLTRNDLYESKKKQFIKQL
                     SLFRERYNV"
     gene            7040..7384
                     /locus_tag="PK733_10150"
     CDS             7040..7384
                     /locus_tag="PK733_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10938.1"
                     /translation="MYRIVRESYENYINDFLPNSASDYRYKIMQPFRLIFDLSAYKEE
                     KNKNSTDYLKLQDFIYLANKNIKEYPNFKSFLWSLESRNIFGKNNGVLSREEFLEQAK
                     IINMFLKLSYWN"
     gene            complement(7436..9652)
                     /gene="feoB"
                     /locus_tag="PK733_10155"
     CDS             complement(7436..9652)
                     /gene="feoB"
                     /locus_tag="PK733_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ10939.1"
                     /translation="MGLTNQSTGAGVLESELKIERISPNDKVIALAGNPNVGKSTVFN
                     SLTGLNQHTGNWPGKTVTNAQGSYIHKDRNFILVDIPGTYSLMANSVEEEVARDFVCF
                     GNPDATIIVTDATCLERNLNLVLQTLEITKNLIVCVNLLDEAKRKKIHINLKELSNRL
                     GVPVVGTNARSSKGLKELMDAVYDITSNKIQINPMRFNYDDLIEEAIKIVEPNLVKIL
                     TEVHRNTLLNEASIDLFSKNVIIDTQRIRWISLKLIDGDKTLLKAMEKYLGFDLMENQ
                     NLLIYIENARRFLSKNGVDHDQLRDRIVSCIVKLAEEISRDTVTFHNEKYNSMDRKID
                     SVLTSKIFGIPIMIGLLGVIFWLTITGANYPSEIIATLLFGIERHLTDFFIWAETPQW
                     VCDLLVMGIYRTLVWVVSVMLPPMAIFFPLFTFLEDLGYLPRVAFNLDSFFKRACAHG
                     KQALTMCMGFGCNAAGIVGCRIIDSPRERLIAIITNNFVPCNGRFPTLIAIITIFFAG
                     MVPGTLKSIVSTLALLIVIILGVVMTFLISNILSKTILKGLPSSFTLELPPYRKPQIG
                     RIIIRSIFDRTLFVLGRAIAVAAPAGLIIWIMANVHVGALSLLDHCAVFLQPFAHLAG
                     LDGYILMAFILSFPANEIVIPIIIMSYMATGNILELDSLNELRDLLLSHGWTWLTAVC
                     VMLFSLMHFPCGTTCWTIKKETQSLKWTIISFLIPTITGIVICFITASVVRLLGLV"
     gene            complement(9685..9921)
                     /locus_tag="PK733_10160"
     CDS             complement(9685..9921)
                     /locus_tag="PK733_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOJ10940.1"
                     /translation="MEIIIPLNNLPLGKKAKVCELKSSGIMRRRMLDLGLILDTEVEA
                     LQKSPSGDPTAYNIRGAVIALRSEEAGNILVRAN"
     gene            10173..10685
                     /locus_tag="PK733_10165"
     CDS             10173..10685
                     /locus_tag="PK733_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOJ10941.1"
                     /translation="MKKEREFHTVRGYQLLGQNKTLLTSALEDYLEMIYRNDQGEGYI
                     RINMIAELLNVRSSSATKMVQKLASMGLLNYEKYGIISLTKKGKEIGEFLLKRHNIIE
                     KFLKTIGVSEKLLVETELIEHNVSLNTLNNISILNSFFEDNPDIFEIFIQYKDKMGDN
                     RDSKCNDKHQ"
     gene            10936..12237
                     /locus_tag="PK733_10170"
     CDS             10936..12237
                     /locus_tag="PK733_10170"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOJ10942.1"
                     /translation="MKMGNSGFNKNNCADRIEKAYEYASSVYRSLGVDTEDILKTMQS
                     MQISLHCWQGDDVTGFETSAEGLSGGGIMATGNYCGKACNGDELRQDMEKAMGLIPGK
                     HRVNLHAIYAETGGKFVDRDELEVIHFSKWIDWAKTRGIGIDFNPTFFSHKNAKSGFT
                     LSSKDKDIRSYWIKHGKVSRKIAAEIGKELGKTCINNVWIPDGSKDLTVSRLEHRLIL
                     RDSLDEIFQQNYDKKHLLDAVESKLFGIGSESYVVGSHEFYMGYALNRNIMLCLDAGH
                     FHPTEGIADKISSILAFSDELLLHISRGVRWDSDHVALLTDDVLAIAQEVKRSNAFDS
                     VHFALDFFDASINRIIAWVVGARSVLKAIMISLLEPTDLLMKEEINGNLGNRLALMEE
                     LKTLPFAAVWDKYCFENNIPVGPAWLNDIEEYEEKVLLRRE"
     gene            12275..13441
                     /locus_tag="PK733_10175"
     CDS             12275..13441
                     /locus_tag="PK733_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ10943.1"
                     /translation="MDCKGFRELERISHVVGNKAEYVQGGGGNTSVKLEQRQGLEQNL
                     NLETKVEFNKLMAVKASGYKLKQITTEDGFVVVNYKNIKEYYEKVDFEQDIDYEKDSV
                     KFTKDNIVKIEGLKELRPSVEAGFHSILKKYVIHTHPVYANIVCCSTNGEELVREIFP
                     EEQWNTLWIPYVNPGFFLTVKIKDGIEKCEKKGKFPEVIFMENHGLIVTSDDMENCIT
                     LHNKVNDQIKKYFKIDKPYPEIKIIRLDKEISKNYNISGGYHITESEDVCGNRDAYIS
                     MTGYLKDFFKGSNLCRNFFEKYVLYPDQLVYLNDSASLDKDENRLNINTLTGELIYRT
                     NYNEALTIEETLLAYIYIIKNIEKCGLKVKSMTLEEIDYIRNWESEKYRKSLMK"
     gene            complement(13585..14334)
                     /locus_tag="PK733_10180"
     CDS             complement(13585..14334)
                     /locus_tag="PK733_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOJ10944.1"
                     /translation="MNYFNNKQNTTFPMQGMPLNMPPCMMMQQGQGQYAAGPQPLMID
                     TEAILGGMYPQDLLMDHNNWAAWGGGIPMDQNFMPVSMQMPINSMPMSPANLNLGTDI
                     CSPALVSPGELQTALELIKEAVSGETEDRLFYEYLISVAPSEEDKNIIKGIRDDEIKH
                     FGMFREIYREFTGENLPIVTDGKFQKPKSYCEGLRDALLGEQRAVEKYRKILFALRDR
                     RQINKLVEIITDELRHGSLYNYLYSKNKCGK"
     gene            complement(14468..>14658)
                     /locus_tag="PK733_10185"
     CDS             complement(14468..>14658)
                     /locus_tag="PK733_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOJ10945.1"
                     /translation="IKDVVLTGNLTNVPQTRNIFDQLGVIFGVNFHIPEHAEYATAAG
                     AAIICAKEMTFTEIRANQ"
BASE COUNT         4727 a         2517 c         2645 g         4769 t
ORIGIN      
        1 ctgaagtgaa tattatacat ggaaatcgtt tcttgccata tttttcttca aatatgaaaa
       61 atgcacccaa atgcaccttg caccttataa agattgttaa atttctgttt aatgatgtat
      121 aatgtgtgat atataatata aagttaaatt attgatggaa atttagaggt gatactatga
      181 gaagacctaa agtacaattt ataacaagaa cagcaatttt gctggcatta actattatgt
      241 tccaaatgat ggggcgtttc ctgggaccta ataacaattt tattgtgggc cctcttgtta
      301 atgctgcctt acttattgca actgctgcaa ccgggctgtg gggggcgaca acaatatcag
      361 ttatagcacc ttttgtttca gctttaacaa ataaagctcc gattgctccg atacttttac
      421 catttactcc ttttatagct attggtaatt ttatattggt actattcttt tacctgttta
      481 tgaaaaagaa caagattgca ggcataataa caggatcagt tctaaagttt tcattcctgt
      541 atgcatcaat cagtgttttc ttaaaagtta tgaaagttcc tgaagggcca tcaaaagtac
      601 tgaattcact tttcagctgg ccgcagctgg taacagcttt ggcaggggga gtaattgcat
      661 tggtagtaat aaaggccctt gggaagaata tagagatgta gaacctctgt cccatttcac
      721 cagcctccat gccgtacgat ctataccatt ggaggctgat gaagtacttg caccaattaa
      781 tatggaatca aaatcaggaa taccactgta aggtattttg atatgcagca tttcgctatc
      841 aggcattagc ctcaggccat caattgatgc tatattttct gaagtatctg tctgcagatc
      901 aatagtctta agataattaa ccgtcatatt tattctgaat gtctttccat cactggagaa
      961 gaaatacata tcgatatagt aattaatatt cctgcgggca ctttcaactg tatccaaaaa
     1021 taaatgcata ttagaactat cagtacttaa atatacattc tttatatctg ctgatttgct
     1081 tgcattttgt tttattttat cgctgacagg atcctctaat aacaatattt tcggaccgtc
     1141 cctcatacta aaatcatact ccaaatcagg atatatgcca aaaagctcat tttgtcttat
     1201 aaacgctttt aaaaacgggc tgtttacatt catttgagac ttgtattcag aaagcatatc
     1261 tgctttttgt ttttgctcaa tatcagacat aggtgaggat acccactttg taccaatatc
     1321 aaccagtttc tttggcggta ctaaaaataa tttactaaac tgtccaaaag gggatggcca
     1381 tttaccccta tgaaccaaat atagatattg atcaggcgtc atattgtttt ttgccaatac
     1441 atacctcacc aggcaatatg cagcaaagtg gtccggatga cggtcattag ggtggggaaa
     1501 ggcaataatg gaaggattat atgtcattac aatactttcc aggtcatcag ctatgctttt
     1561 tccacaatat ggagcatcgc ttttgtatga gttgctatag ggtgaatggc tgcacttggt
     1621 aaaccggctt acatataggt tgttttcatc ccagttttca ccccacatat ggtataggcc
     1681 accatcagga tatcccaggt aaataatgtt ttctctatca acacctgctc ttttcatggc
     1741 tctctctgtt tcgtcctgcc gcaaatatgc ggaacttata taattttttg cgttcggctt
     1801 gacagtcagg taatttaatg ctacagcact tgtatgtcca tctccatttg tcaaaaggac
     1861 aacttttacc gtaccccctg attctatagt ttttgaaatc aagtttccac atccaagtgt
     1921 ttcatcatcg caatgaggag caaccacaag cagccttgtt ccttcaactg gtgacacaag
     1981 ctcatccatt tgaagctttg taaataaatt aacaataaag tctctaccaa atactcctgt
     2041 taccaatatt atgatagcta aaccgagaaa gatatatttt aacttattag caattttttt
     2101 cttattgata gattgccttt tatccacttt aattttatca acctcatatc tttttatttt
     2161 agtatttaga tttagtctat atttttaata aatattacag ttttcttaga agatattaca
     2221 gttataaaaa tattacagtt ttttaacaaa tattattgtt ttgtaatctt ctacatttcc
     2281 ccatagcgta tcggaaaatt caagcagttt agtatatcca ttttttatat agaacttgat
     2341 agcactgcag tttttattgg aagttttaag atgaattcct ttttcctgta tgccggggtt
     2401 gttttttata tgattttcaa aagtggccag cagtgatgta cctatagacc tgccctggta
     2461 ttcaggcaac atatttatat gtaaatgagc aggatattca ttatataagt tttttggcat
     2521 atatttatat gttttatttt taagcatatt tcttatactt tttaatatag aaggatacat
     2581 gcaaaaagta tataataatc ttaacccgat tctccaggac attttcatca tgaacattat
     2641 tatttgcctt ttagtattca tagtacctat aatataccca acaaccatat tatcatgttc
     2701 actatctact gcaacaaagc aatgctcttt ttcataaaga agataataag cacaaaagaa
     2761 atagccgaaa agttttctgt cgccgaactc tccggtacct tccaaatctt ttcccatata
     2821 gcctgtttta tggcatatat ttaatacctg ttccaagtcg gtatcattat attttctaat
     2881 aagtatttcc ggcataccat atcctttttc gcttaactat gtattatgct ttatattatt
     2941 actactttgt tatgctgcca attggcatgg tagcattaat gtattattaa ttttaacata
     3001 ttatcaaata ataatgtata agtttttcaa aataaggaag gacagtccca taaaaagaac
     3061 tgtccccatc ataatagtgg agaactgtcc ctcatttatt tctcatttaa ttttattttt
     3121 taactccgta aatttatttt taaacttgga atttctatca agagccattc ctgtatcaat
     3181 agcagttaat gcatctcttt ttgtccctgc ttttaccagg gcatcggcaa gattacacca
     3241 gttgcaaact gtatatggtt cctttctgat tctttgaaca aaatagggga ttgctttttc
     3301 attatagtaa gtggtaatat tttcaacagg ttccagcctt agaccattcc atcccagcac
     3361 ttcaatattg taagtaccgt cattttgcct ggtccaaacc ccaagttctg cttttgattg
     3421 tgcagccctg ttttttgatt cacggagtac gtcaagccta tcatagttcc gtgaaaagag
     3481 ttcatttgtt ttaccatttt ccagtgaata tccatatacc tgtgaaggtt ttcctcttga
     3541 tgataaacct aatatgagct gctttttacc tgcccctgta agatcagcaa cacttctatg
     3601 atctaatatt tcatattcat tattcgttat ttctgcagcc gtataccagc tatcattttg
     3661 ttttttaaga ataacagttc ttatttcgtc attaagtttg aatgaagtaa tcaactcttc
     3721 agataaatca ccatcaatgt ccgacaggtg aatttccctg gtgtatgaag gactttcagg
     3781 tagtaaaagt ttcgtgttct caggaacaaa ttttttcgcg attgtataat aactctctgt
     3841 tttccggttt agcttggaag cgctttcttt caactgtgaa aaggacgatg ttagagtttt
     3901 gcttaatgat tccagagagt ttgcaatagc catgggtata accatatttt tagatgcttg
     3961 attttgtgtg cctgatcccg gttctctttg ggtatctggt ttataattgt tatattttgt
     4021 taataatgcc ctccccgcaa gtgcaagtcc ggctaattgt aatggaagca taagttatat
     4081 cacctttgca gatatattca tatagtatta tatgcaatga agatgaatat gggaccatgt
     4141 aaggaaagga caatataatt tatggttaaa aacattcgag tcacagaatc tatgaatcat
     4201 acactgattc tatgacccgg gaaatcagca gtttgactta taagacccta attccaggcc
     4261 tttctcaata aaatacttat atgtatcata atcaacggta tttggaattg aatggtcgct
     4321 atgaagggcg tacccataat tccccatagc tacaggtatt ttagattcta attctttttt
     4381 tattagttct ttatcatttg tgtatagaac tcttacatca ataccaccca taaaagacaa
     4441 aatatcccca tattccctat ataatttaag gagatccata ccggctttta cttctataac
     4501 ctgaaggcaa tccatacctg cttcaattaa ccccggaaca agagattcta ccataccgca
     4561 tgaatgaaca attacaggca gatcatgtgc atgagcgaaa tcaaaagttc ttttatgacc
     4621 cggttgtata atttccttat acatggcagg tgagaagaat ggtttgcctt taaaacccat
     4681 atcttcatag taccagattc catcagggta tccttcttca gcaaatagaa tttcctgaag
     4741 gtccatagtc agacggctgt atgtatctac catatctata atccaatccg ggtcaagagc
     4801 cattcctatt aacatatact catgaccgca aacaggatgc attaattcaa atacatttac
     4861 acccgaccat acaaaaaaac gatttttctc tgcagcatat ttcttcgctt ttctgtatgt
     4921 ttcgaaattt atacgcctgc gttcaggctt caatagcggc tttatatgtt cttcccaatc
     4981 tactcgctcc tttacaagaa aatccacatg ctcgggagta gtatcatgaa gcttgtgtct
     5041 gcgcaggagg gcaccattac catctcttac aagtatagtc tcttcagttt cttctatagt
     5101 ttccggttta aaatccaaat tggcagtcat attaaatgcc caacattcat ccaggtcaaa
     5161 atcaaaatga tcggcgaagc cttcgctttc tttgatatat ccgtccgaag tccatttttt
     5221 ataggtatca ctccagaaat gttcgtaaag tcctatatgg tcaacaggtt tacgctttag
     5281 tatattactc atacgttcaa taccggtaat agcttgcata tgctgcacct ccaatgctta
     5341 ttcagtgata tattcaacat ttacattttg caattaacga attatatata gcatatagca
     5401 tccgaacatt aaattcaatt gaaaaaggta acatttctaa actgcaaaat aaactacaaa
     5461 aaatccataa tttctaatag aaaaagatgt aataagccat aaaatttaag ttgtatatat
     5521 ttatttgtaa tgataatgga tttctgcaca ggtttgcata atgaacagat atgatataat
     5581 taaataatga acaagatatt ttttaagtga acggtggata agacactttt taaatggggg
     5641 agtagaaatg gattttatgc ttctggataa tataaaagtt gaaatcagca atgaggaatt
     5701 atttaaatca ctaaaaatag atatggagga cgatattgca ccggaagtgc ttggattgtt
     5761 tgaagaagca tggaaaacag ccagacctaa ggcagtatat ggggctgctt atatagaaga
     5821 ccgcgggaga aactgggtag ttatcaatgg tatcaggttt acaagcagga tattgagtgt
     5881 aaatctgaaa gatgcacata gagtgtttcc ttatgttgtt acatgcggga gggagctata
     5941 cgaatgggct atgtctatta atggccctct agagcgatat tgggcggaca ctatatgtga
     6001 aattttcctg cgcaaagcgt ttaaaacgat gagcgaggat tttattaaaa agttcaatcc
     6061 gggcaaaact gcaactatga atccgggatc actggaggac tggcctataa gtgaacaaaa
     6121 accgttgttt tcgttaatag gtgacgataa tatcaaaacc gcaggtattg agttgactga
     6181 aagttttctt atgattcctg ttaaatccat gtcaggtatc aagttcctaa cagagtataa
     6241 gtatgaaaat tgtatgttat gtccaaggga aagatgtcct ggaaggaggg ctgaatatga
     6301 ttcgctgttg aaagaaaaac tatgatataa tatcctcatc ctcataaatg aggatattat
     6361 atgcctccgg cggaatttat gtgcatgcga atatttttgc ttcgcaaaaa acgcacatgc
     6421 acaattatat catgaatttg ttaaaattca tgatataatg tacgtgttat aataaattca
     6481 aaatatgtta aatttgataa atataatggt tttatgtaac gtgatatggt gaaatatatg
     6541 gatgtaaaga aacaattgga agaaaaaatt tccgatatgg agaaggtagc aagaattttt
     6601 gaaattgagc cttttgacat atactttttg ggcggttctg cctgtatatt aggtggttat
     6661 acagataggg ccacgagaga ttttgatttt atagatttaa attatccttc aagactaggg
     6721 aaggtttttg tacaattaag agattttgac atgttggaat atgaaagtac tattatatca
     6781 cctaaatata aagagcgggc tataaaacta aatgaattta aatatctaaa tatttatttg
     6841 ctatctgtag aggacattat tgttagcaaa ataatcagac tggaacaaaa ggacttgcaa
     6901 gacatttatg aacttattaa agttgcagat aaagagctta taaataaaat tattgatgaa
     6961 gttttaacta gaaatgattt atatgaatcg aagaaaaagc aatttataaa gcagctttct
     7021 ttatttaggg agagatataa tgtatagaat agtacgtgaa agttatgaga attatataaa
     7081 tgattttttg ccaaacagcg catctgatta tcgttataag attatgcagc cttttagatt
     7141 gatttttgac ttatctgcgt acaaagaaga gaaaaacaaa aacagtacag attacttgaa
     7201 attacaagat tttatatatt tagcaaataa aaatattaaa gaatatccga attttaaatc
     7261 ctttttgtgg agcctggaat caagaaatat atttggtaag aataatggag ttttatcccg
     7321 ggaagagttt ctagaacaag ctaaaattat aaacatgttt ttaaaattgt cttattggaa
     7381 ttgaatagaa ttcgcgtggt aaatggttta gtacgacatt gcgtccacac ctaaattaaa
     7441 ccaatcccag caggcgcaca acgcttgctg taataaaaca tattacaatt cctgtaatgg
     7501 taggtataag gaaggatatt atagtccatt ttaaactctg agtttccttt tttattgtcc
     7561 aacaggtagt accacatggg aagtgcatta gagagaatag catgacacat acggcagtaa
     7621 gccaggtcca tccatgtgac aaaagtaaat cccgcagctc attaagactg tcaagttcta
     7681 aaatattgcc tgttgccata taactcataa taattatagg tattacaatt tcattagcag
     7741 ggaaactgag tatgaaggcc atgagaatgt atccatccag acctgctaga tgagcaaagg
     7801 gttgcagaaa aactgcacag tggtccagca ggcttaatgc tccaacatgg acattagcca
     7861 taatccaaat aataagacct gcaggagcgg caactgcaat agcccttcca aggacgaata
     7921 aagttctgtc gaatatggaa cgaataatta tcctgccgat ttgcggctta cggtatggtg
     7981 gtagttccag ggtaaatgat gatgggagcc cttttaaaat tgtttttgat aatatatttg
     8041 atattaaaaa tgtcattact acaccaagaa ttataacaat taatagtgct aaagttgata
     8101 caatagactt taatgttcct ggtaccattc ctgcgaaaaa tattgttatt attgctatta
     8161 gtgtagggaa ccgcccattg catggtacaa aattattagt aattatagca ataagacgct
     8221 ctctgggaga atcaataatt ctgcatccta caatccctgc agcattgcat ccaaagccca
     8281 tacacatggt taaagcctgc tttccatgtg cacatgccct tttaaaaaag ctgtcaaggt
     8341 taaaagccac ccttggtaaa tatcccagat cctccagaaa cgtaaacagt ggaaagaaaa
     8401 ttgccatggg ggggagcata acagatacta cccacacaag tgtcctatat atacccatca
     8461 ccagcagatc gcagacccat tgaggagtct ctgcccatat aaagaaatct gtcagatgtc
     8521 tttcaatccc aaaaagcaaa gttgcaatta tttctgaggg gtaattggct ccggttatag
     8581 ttaaccagaa tatgacacct aaaagtccaa tcattatagg tatgccaaat atttttgaag
     8641 tcaggacgct atcaattttt ctatccatac tattatattt ttcattatga aatgtgaccg
     8701 tatccctgct gatttcttct gcaagcttta ctatacagga aacgatccta tctctcagtt
     8761 gatcatgatc aactccattt ttacttaaaa accttcttgc attttctata tagattagta
     8821 aattctggtt ttccattaaa tcaaaaccaa gatatttctc catggcttta agcagtgttt
     8881 tgtcgccatc aattaatttt agtgaaatcc aacgaattct ctgcgtatct ataataacat
     8941 ttttggagaa aaggtctata gaagcttcat tcaaaagggt gtttctgtgt acctccgtta
     9001 atatttttac taaattgggc tcaacaatct taatagcctc ttctattaaa tcatcatagt
     9061 tgaatctcat gggatttatt tgaattttat tgcttgttat atcatatact gcatccatta
     9121 attcttttaa gcctttgctg cttcttgcat ttgtacctac cacaggtaca ccaaggcggt
     9181 ttgagagttc cttcaagtta atgtgtattt tttttctttt tgcttcatct aaaaggttta
     9241 cgcacacaat caaattcttt gttatttcca gagtttgaag cacaagattc aggttccttt
     9301 caagacatgt agcatctgta actattatag tggcgtcagg attgccaaaa catacaaaat
     9361 cccttgctac ctcttcttct acagaatttg ccatcaatga gtaggttcct ggtatatcta
     9421 ccagtatgaa atttctatct ttatggatgt aacttccctg agcatttgtg acagtcttgc
     9481 ctggccagtt gcccgtatgc tgatttaaac ctgttaaact gttaaaaacc gtgcttttac
     9541 ctacattcgg gttgcctgcc agtgcaatca ccttatcatt aggtgatata cgctctattt
     9601 ttagttcaga ttcaagaaca cctgctcctg tagattgatt tgtcagtccc atattccaat
     9661 tccccctgat tattttatgc agtattaatt tgcccgtaca agaatgttcc ctgcttcctc
     9721 cgaacgcagt gcaatgacag cacctcttat gttataggca gtagggtcac ctgaagggct
     9781 tttctgcaga gcctcaactt ccgtatctaa aatcaaacca agatccagca ttcttcttcg
     9841 catgattcca ctggatttaa gttcacatac tttagccttt tttccaagtg gcaaattatt
     9901 tagtggtata ataatttcca tagttctaca aacccccatt ttatacttgt attataaaag
     9961 ctttatttca tatttgcatt acaaaggcta cgttttcata catgaattac aaaactttgc
    10021 ccggctcttt atgttcatat attatttccc ctaaataagc cgcgagaaaa tatctttctc
    10081 aagactaata tatgaaaaat aaaatgcaag tgttacgaat atacattaaa taagttatgt
    10141 agattcatat ttatgaaatg gagtgatact atatgaaaaa ggaacgtgaa tttcatacgg
    10201 ttcgtggcta tcagcttctc ggacagaata aaactctttt aacttctgca ctggaagatt
    10261 accttgaaat gatatacaga aatgatcaag gggaaggtta tattaggata aatatgattg
    10321 ctgagctatt gaatgtaagg tcttcatcag ctacaaaaat ggtccagaag ctagcttcca
    10381 tgggactttt gaattacgag aaatacggca ttatctcctt aactaaaaaa ggcaaagaaa
    10441 ttggcgagtt tcttcttaaa agacataata tcatagagaa atttttaaaa actatagggg
    10501 ttagtgaaaa actgctggtt gaaacagaat tgatagagca taatgtaagc ttaaacacgt
    10561 taaataatat ttccatttta aatagcttct ttgaggataa ccctgatatc tttgaaatat
    10621 ttatacaata taaggataaa atgggtgata atagagatag taaatgcaat gataagcacc
    10681 aatagctaac ttgcgtatat taatagagaa aaaaaagcaa tcaagcctct tacaatgcac
    10741 ccttaaattc aggggcacat ttaaaatttt taaagaaata tactccggga aattcagata
    10801 gggtttgact tttatatggt aatttgtata ataccgttat taatttagct gtgaattgtg
    10861 agcaacttat cttatgcaac cggtaatatg atataattat aagtgtttta tatttatatt
    10921 ccaagaacag ggggcatgaa gatgggtaat tccggcttta ataaaaataa ttgtgcagat
    10981 agaattgaaa aggcttatga atatgcaagc agtgtttaca ggtcattagg tgtggatact
    11041 gaagatatat tgaaaacaat gcaaagcatg caaatctccc ttcactgctg gcagggtgat
    11101 gatgtaacag ggtttgagac aagtgcggaa ggactttccg gtggtggaat tatggcaact
    11161 ggcaattatt gtggtaaggc ttgcaatgga gatgagctaa ggcaggacat ggaaaaagca
    11221 atgggattga taccaggtaa gcaccgtgta aacctgcatg caatctatgc tgaaaccgga
    11281 ggtaaatttg ttgatagaga tgaacttgag gttatacatt tttcaaaatg gatagattgg
    11341 gccaaaacaa ggggaatagg aattgatttt aaccctacgt ttttttctca taaaaatgcc
    11401 aaaagtggtt ttacattgtc cagcaaagat aaagacatac ggtcatactg gataaagcat
    11461 ggaaaagtat cacgtaaaat tgccgctgaa atagggaaag aacttggtaa gacatgtata
    11521 aataatgtat ggattcctga tggttcaaag gacctcacgg taagccgttt agaacatcgg
    11581 cttatattaa gggactctct tgatgagatc tttcaacaga attatgacaa aaagcacctg
    11641 ctggatgcag ttgaaagcaa gctctttggt ataggctccg aaagctatgt agttggatca
    11701 catgaatttt atatggggta tgctttgaat agaaatatta tgctttgcct tgacgcaggg
    11761 catttccatc ctacagaagg tatagcggat aaaatttcat caattcttgc tttttcagat
    11821 gaattgctgc tgcatataag caggggtgta agatgggaca gcgatcatgt agctctatta
    11881 actgatgatg tccttgcaat tgctcaggaa gtaaagagaa gcaatgcttt cgatagtgtg
    11941 cactttgccc ttgacttttt tgacgcaagt attaacagaa ttatagcatg ggtagtggga
    12001 gcaagatcag ttctcaaagc cataatgatt tccctgcttg aacctactga tttattaatg
    12061 aaagaagaaa tcaatggaaa cctgggaaat cgtcttgcct taatggaaga attaaagacc
    12121 ctgccctttg ctgctgtttg ggataaatac tgctttgaaa ataatatacc tgttggcccg
    12181 gcctggctta atgatataga agaatatgaa gaaaaggttt tgcttagaag ggaataacgc
    12241 aaaatcataa ataaaagaag gaggaggaga tggaatggat tgcaaaggct tcagagaatt
    12301 agaaagaatt tcgcatgtag ttggtaataa agctgaatat gttcaggggg gcggaggcaa
    12361 tacttcagtc aagctcgagc agaggcaggg cctggagcag aatctaaatc ttgaaactaa
    12421 agttgaattt aacaagttga tggctgtaaa agcgtcgggg tataagttaa agcaaattac
    12481 cacagaagac gggttcgtag ttgttaacta taaaaatata aaagagtatt atgagaaagt
    12541 tgattttgag caggacattg attatgaaaa agatagcgtt aagtttacta aagataatat
    12601 tgtaaaaatc gaagggctaa aggaattgcg accttctgtg gaagccgggt ttcattcgat
    12661 tcttaaaaaa tatgtaatac atacccaccc tgtttatgcc aatattgtat gctgctctac
    12721 taatggagaa gaactggtac gagagatatt cccggaagaa caatggaata cattatggat
    12781 accatatgtt aaccccggct tttttctaac ggtaaaaata aaagatggca ttgaaaaatg
    12841 tgagaagaag ggaaaatttc ccgaggtaat ctttatggag aaccacggac tgatagttac
    12901 atcagatgat atggaaaact gtataactct ccataataaa gtaaatgacc aaattaagaa
    12961 atattttaaa atagataaac cttatcctga aatcaaaatt ataagattgg ataaagaaat
    13021 atccaagaat tataatatat caggaggtta tcatataact gaaagtgagg atgtatgcgg
    13081 gaacagagat gcatatataa gcatgaccgg atatttgaaa gactttttta aaggaagcaa
    13141 tttatgcagg aacttttttg aaaagtatgt tctttatccc gatcagcttg tatatctaaa
    13201 tgacagtgcc tctttagaca aagatgaaaa taggcttaat ataaatacat taacaggtga
    13261 gttaatatat agaacaaact ataatgaagc tcttactatt gaggaaacat tacttgcata
    13321 tatctatatt ataaaaaaca ttgagaaatg cggattaaaa gtcaaatcta tgaccctgga
    13381 ggaaattgac tatatcagga attgggaaag tgaaaagtat aggaaaagtt tgatgaaata
    13441 agcaagtgaa gaggagcggg tgcatttaat ttatatgaag aaaatattgg taaggggcat
    13501 aagtggagat gttctaagca ccgtaatgac agcccctgat aaatatttct tcatatttga
    13561 taaatgcatc ccatatgctt tcactcactt accacattta ttttttgaat aaaggtaatt
    13621 atacagtgac ccgtgtctta attcatcagt tataatttca accagtttat ttatttgacg
    13681 tctgtctcta agagcaaaaa gaatcttcct gtatttctca acagctcttt gttcgcctaa
    13741 aagcgcatcc cgtaaaccct cacagtagga tttcggtttc tgaaactttc catctgtgac
    13801 aatgggaagg ttttcacctg taaattccct gtaaatctct ctgaacatgc caaagtgctt
    13861 gatttcatca tctcttatgc cttttattat gtttttgtct tcttcgctgg gagcaacact
    13921 tattaagtat tcataaaaca atctgtcttc agtttcccct gaaacagctt ccttaataag
    13981 ctcaagagct gtttgcaatt cacctggtga tactaaagca ggtgaacata tatctgttcc
    14041 caaattaaga tttgcagggg acattggcat actgttgatg ggcatttgca ttgatacagg
    14101 cataaaattc tgatccatgg gaataccgcc tccccatgca gcccagttgt tatgatccat
    14161 tagaagatcc tggggataca ttccgcccaa tattgcttct gtatcaatca taaggggctg
    14221 ggggcctgcg gcatattgtc cctgtccctg ctgcatcatc atacatgggg gcatattcaa
    14281 gggcatacct tgcatcggaa atgtagtgtt ttgtttgtta ttaaaatagt tcatattatt
    14341 accaacctca tacaatacat aatatcttct atagttatac tatgaaggga aataatagat
    14401 tgttacaggt taatttactg ctttttatgt gtagacttaa ttaattctca aggtcaagtt
    14461 gcttctatta ctggtttgct cttatttcag tgaaagtcat ttccttagca caaataattg
    14521 cggctcctgc agcagtagca tattctgcat gttcgggtat atgaaaattt acaccaaaaa
    14581 tgactcccaa ctggtcgaat atgtttcttg tctgaggaac attggtcagg ttgccggtca
    14641 aaacaacatc ttttatgt
//
LOCUS       DAOSDD010000100        14438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1656_length_14438_cov_5.461308, whole genome shotgun sequence.
ACCESSION   DAOSDD010000100 DAOSDD010000000
VERSION     DAOSDD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1656_length_14438_cov_5.461308"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK733_10190"
     CDS             <1..259
                     /locus_tag="PK733_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase"
                     /protein_id="HOJ10946.1"
                     /translation="FVSMVEKGDIEALMESKSLWICLSCFTCVERCPRNVEPAKLIEA
                     VRLAAIRKQGENYLKADDIPLLLDENIPQQAIVSAFRKYSK"
     gene            601..2586
                     /locus_tag="PK733_10195"
     CDS             601..2586
                     /locus_tag="PK733_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOJ10947.1"
                     /translation="MQKIGVFVCWCGSNIAATVDVEAVVNALKNEPGVVYAADYQYMC
                     SEGGQSKIRHAIKDFGLTGIVVCSCSPRMHEATFRKTVASAGLNPYMVEIANIREQCS
                     WIHKNKEEATQKAIILARTAVAKLNLNKPLKAGESAVVKRALVIGGGIAGIQSALDIA
                     EAGYEVDIVEKSPTIGGKMAQLDKTFPTLDCAACILTPKMVDAASNEKINLYTYSEVE
                     AVSGFVGNFTVKIGIKARSVKADLCSGCGVCTEKCPSRKAKSEFNMGLSNRGAIYIPF
                     AQAIPNVPVIDREQCIKFKTGKCGICEKNCSAGAIDFTQQDKIVEKQYGAIVVATGFN
                     PISLKNFDEFSYSRCPDVVTSLEFERIMNAAGPTKGKLLRPSDNTHPRTIVFVQCVGS
                     RDTSGCGKTYCSKICCMYTAKHAMLVREKYPYVDVHVFYIDVRTPGKNFDEFYRRAVE
                     EYGVNYIKGMVGKIAEKNGRLMVQGSDLLADEQIVIEADMVVLATAIEPDKTAKDLAT
                     MLTASIDTDNFFTEAHPKLRPVESPTAGIYLSGVCQGPKDIPETVAQASAAAAKVIGL
                     LAKDKLLCNPCVSSSDEMLCNGCSSCEKVCPYGAISYVDKEFRAAGKIEVRRVAQVNE
                     AVCQGCGACTVACPSSAMDLKGFTNKQVMAEVDAICR"
     gene            2577..3023
                     /locus_tag="PK733_10200"
     CDS             2577..3023
                     /locus_tag="PK733_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOJ10948.1"
                     /translation="MPIKDEFRPLIVAFCCNWCSYAGADLAGTSRLSYPADVKIIRVP
                     CSGRINPMFILRAFQRGADGVIICGCHPGDCHYSTGNYYARRRMTLLFSLLDYLGIER
                     ERTRVDWVSAAEGVKFSTVMNEFSKTIHQLGENKRLGDLRCKEFLD"
     gene            3002..4165
                     /locus_tag="PK733_10205"
     CDS             3002..4165
                     /locus_tag="PK733_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ10949.1"
                     /translation="MQRIFGLIKDKTEKLLADGTVNRVIGWKKGELFYDNTPAVFNIS
                     TVPGTTLTGDEMESFVYDGFCASNLSKYLISESKKDGKILALLKPCDTYCFNQLAKEH
                     RINKDNVYILGIECRGMIDINKIKDLGIKGIISIEEKDYEYEKYDQLIIQTIYGEKTC
                     KKREVLLEKCLCCKGKRHVTYDELLSRGDHTPEDTRADIRVLTGENTPEVTPEVTHED
                     TSKDIRKGTPEATRKKASQLDSNRFELVEKLEAMTPDERFSFWRGELSKCIRCNACRN
                     VCPACSCIKCVFDNDNSGIASKANTDTFEENMFHIIRAFHVAGRCTDCGECSRVCPQH
                     IPLHLLNRKIIKDINELYGEYQAGADTESRAPLADFTKEDVEPSIVAKKGGEH"
     gene            4165..5358
                     /locus_tag="PK733_10210"
     CDS             4165..5358
                     /locus_tag="PK733_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ10950.1"
                     /translation="MFKISTQCLTELFSLISDNRALYMPIEKNGQVQFGKWQRNEKVR
                     LDKLNAVKSPKEFFFPQSENIVAFKTKGKNISIIDNRDESEAFVVFGVRACDAASLGI
                     LDRVFLAEPVDTFYKTRRDKGIIITLACSEPEETCFCSVFGIDAANPAGDVATWIIGD
                     VLYWKSLTPKGEALTETVKSKSKINSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNG
                     NGNSNSNSIFEMADSSDEEKLEGYKENVKEIIQKLPFYDLSLDSFNSEVLGEALKDKF
                     NSPKWKELSQSCIGCGTCTFVCPTCQCYDIRDYDTGHGIQRFRCWDSCMYSDFTKMAH
                     GNPRTSQLERFRQRFMHKLVYFPANNDGVYSCVGCGRCVAKCPVSMNIVKVIKAMGVK
                     ESVRL"
     gene            5417..6226
                     /locus_tag="PK733_10215"
     CDS             5417..6226
                     /locus_tag="PK733_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOJ10951.1"
                     /translation="MPKLGVVTDIKIDTPDVKTFRVNALDGGVCFKHMPGQCAMLSIP
                     GVGEAMFSITSSPTNTEYMEFSIKKCGHLTEWLHQIDMGQQITIRGPYGNAFPMETEL
                     KGKDLLFIAGGIGLAPLRSVINYARHYRKNYGHIDIVYGSRSKDDLVNYDEIINEWCN
                     DDGINVYLTIDREQEGWDGHVGFVPNYIKELNFNTNKTAIVCGPPIMIKFTLVALQEL
                     GFDKTQIFTTMELKMKCGVGKCGRCNIGSKYVCKDGPVFRCDELDELPNEY"
     gene            6519..7193
                     /locus_tag="PK733_10220"
     CDS             6519..7193
                     /locus_tag="PK733_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOJ10952.1"
                     /translation="MEEMVNVFLMGKKYAVPSNLTIMGAMEYAGYQLVRGCGCRNGFC
                     GACAIIYRIKGERELKVCLACQTKVEDNMYIATLPFFPLVKEVYNINEIKPTEQIMMQ
                     LYPEIYSCIGCNACTKSCTQGLDVMQYIAYAQRGEYKKCAEASFDCVMCGVCSSRCPA
                     GISHPQVALLARRLTGKYLAPKSQHLENRVCEIGNGDFKELLEGLMQKPIEKLKELYN
                     NREIEE"
     gene            7376..8977
                     /locus_tag="PK733_10225"
     CDS             7376..8977
                     /locus_tag="PK733_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ10953.1"
                     /translation="MYTQDMLESIRKVEATRKERLSMEPERMTADEKEVLLKKYHPDY
                     RQENFSVLKIGPNKGDKVPDELADLLQANSRIKDVDIDLSKVDYDIDVLIIGGGGAGA
                     SAAIEANEGGADVMIVTKLRIGDANTMMAEGGIQAADKENDSPMIHYLDALGGGHYAN
                     KPELLNKLVTEGPSAVKWLNDLGVMFDKAPDGTMITTHGGGTSRKRMHAAKDYSGSEI
                     MRVLRDEVINRGITVVDFTAAVELILDDEGKAAGAILMNMETKEYMVARAKTIIIATG
                     GAGRLHYQGFPTSNHYGATADGLILGYRAGAKLLYADTIQYHPTGAAFPEQIFGALVT
                     EKVRSLGAMLVNADGEAFMHPLETRDVCAASIIRECKKREKGVSTLEGSGVWLDTPMI
                     EIINGEGTIEKRLPGMLRMYKKYGIDITKVPILVYPTLHYQNGGLQINAEGLTAIDNL
                     YAAGEVVGGIHGRNRLMGNSLLDIIVFGRSAGKNAAKKCKNVKVGKLTLDHVNKYAKE
                     MESAGVKSNCASPKLLPTYTHRQVK"
     gene            9118..9393
                     /locus_tag="PK733_10230"
     CDS             9118..9393
                     /locus_tag="PK733_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HOJ10954.1"
                     /translation="MQGLASVLETFMIVSFGVSWPISIIKSHRSRSTKGKSLFFMCFI
                     LFGYICGIASKCISNTYNLAFWFYFPNVIMVATDICLYFRNKRLEAK"
     gene            complement(9550..10704)
                     /locus_tag="PK733_10235"
     CDS             complement(9550..10704)
                     /locus_tag="PK733_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ10955.1"
                     /translation="MANSELLNKFKEKISDTKNRDTSKKLKVGIIGTGWIAEYHLIAY
                     KNMPDVEVVAGADLIEGKAKKFFEDHDVKGARVYLSHKEMLDNEELDAVSVCTYNATH
                     AECTIYALKKGVNVLLEKPMCVTLDEAIEIMRAEKESGKVLSIGFQPRFDENMKMIKK
                     IVESGELGEIYYIQTGGGRRRGIPTPYGTSFIEKETAGIGALGDIGCYSLDMVLNAIG
                     YPKPLTVTGYKSDFFGKSKEYSNKPEYADIFGVDDFAAAFIRLEGGIILDFRIAWAMH
                     PDTTGDTIIFGKKGALRIPSTECWNGSVGGAMTIYHDVAGLQTETKVPILKTKEDLFN
                     LKVRSFLDAIIDGTSAPVPASQILYNQAIIDGISKSAELGREIEIVIPEI"
     gene            complement(10924..11757)
                     /locus_tag="PK733_10240"
     CDS             complement(10924..11757)
                     /locus_tag="PK733_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ10956.1"
                     /translation="MPLSIGLQLYTLRNEMEKDFKGTLEKVKEMGYEGVELAGLFGKK
                     GKEIKKMLDELSLKAVSAHVPLDELLEEPDKTFQTYKDIGLEYIAIPHLPEEDRPQAG
                     NFDTTLKNITFICEKAKEYGITMLYHNHNFEFDIVDGEYYLDTIYKSISKDLLQTQID
                     TCWVNVAGVNPSEYLMKYQDRAPIVHLKDFVMKNRARKENLYEFLGTNSSVEIDEDVK
                     EDFAFRPVGYGVQDFKSILDACKKVNTKWVIVEQDRPAKGQTELESAKLSCEFIKNLG
                     L"
     gene            11989..12810
                     /locus_tag="PK733_10245"
     CDS             11989..12810
                     /locus_tag="PK733_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10957.1"
                     /translation="MVAFYENKDKDLTFNYGSRLDFRAHLHKHIELVYMLEGETITYV
                     DGKKAILKEGDVFISFPNQIHQYFSKGPEKYLIIIFSPDILPEMKNIFINYIPESQVI
                     EKAYKCPYLYTILSSVVKAYESEYEYRGLQVKGYLLTFFGELLKNMKLTPIKASNLSM
                     LQSILNYCSENYMKDLSLESISDALHVSKYYVSHLLKKKINIGFSDYINSLRISAACD
                     HLLSEEKSITEIAYFVGFNTTRTFNRAFIKHTGQTPTYFRASMSGAKCTIMNKYM"
     gene            13043..13207
                     /locus_tag="PK733_10250"
     CDS             13043..13207
                     /locus_tag="PK733_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10958.1"
                     /translation="MSYIKTVLPMEGYRLLMERGNGSTVIADLSSKLHTMKYADLADK
                     VFLITATTEG"
     gene            13218..13424
                     /locus_tag="PK733_10255"
     CDS             13218..13424
                     /locus_tag="PK733_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10959.1"
                     /translation="MPEDTVMLVARQKLFKADICNPWKLGYESSDADLLYALYADPIL
                     YADPIKRWERPTYEESRNMKRVEI"
     gene            complement(13603..13965)
                     /locus_tag="PK733_10260"
     CDS             complement(13603..13965)
                     /locus_tag="PK733_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10960.1"
                     /translation="MAANPVYHPDLCNRNNICAHSTVRLSDAGGALSYFVLIGALLLI
                     WAAKRAHSHWKSITWGLVTMVALLVGGQAIAVISGLTSGAIEPAGWIWALVITSIALY
                     SLTLIVIGIAGVLLVREL"
BASE COUNT         4641 a         2653 c         3026 g         4118 t
ORIGIN      
        1 attcgtttct atggtggaaa aaggcgacat tgaggcactt atggagtcaa aatcactttg
       61 gatatgtctt tcgtgcttta cctgtgtgga gcgctgccct agaaacgtcg agcctgcaaa
      121 gcttattgaa gctgtaagac tggcagcaat acgcaaacaa ggggaaaatt atttaaaagc
      181 agatgacatt ccgttattat tggatgaaaa tattccacaa caggcaattg tcagcgcatt
      241 cagaaaatac agtaaataat cagcatataa gtactgcttt actatgtttc caaattggca
      301 tagcagtatt atattgtaaa taattggcga attacattat aagtaattag caaatcagat
      361 tataagtaat cagcaaatca gattataagt aatcagcaaa tcagattata agtaatcagc
      421 aaattagatt gtaaataatc agcaaattag agcaaattag attgtaaata atcagcaaat
      481 tagagcaaat tagattgtaa ataatcggtg atttagattg taaataatcg gtgatttaga
      541 ttgtaaataa ttagtaaatt agattgttaa taatcagcac attagattgg aagtgagtaa
      601 atgcaaaaaa taggcgtgtt tgtttgctgg tgcggaagca acattgctgc aactgtcgat
      661 gtagaagcgg ttgttaatgc tttaaagaat gaacccggtg ttgtttatgc ggccgattat
      721 cagtatatgt gctccgaagg cggtcagtca aaaatcagac atgcaatcaa agactttgga
      781 cttacaggca ttgttgtttg ctcatgctct ccaagaatgc atgaggcgac cttcaggaaa
      841 acagttgcta gtgcgggatt aaacccgtat atggttgaaa ttgcaaatat ccgtgagcaa
      901 tgttcgtgga tccacaagaa caaggaagaa gcaacccaaa aagcaattat tcttgcgaga
      961 actgcagttg caaagcttaa cttaaataag ccgctgaaag caggagaaag tgctgttgta
     1021 aagcgtgcac tggttatcgg cgggggtatt gcgggcatcc agtctgcact tgatattgca
     1081 gaagccggat atgaagtaga tattgtagaa aagtcaccta caataggggg caaaatggca
     1141 cagcttgata agacattccc gacccttgac tgtgcggctt gtatccttac acccaaaatg
     1201 gttgacgcag catcaaacga aaagataaac ctctatacat acagtgaggt cgaagctgtt
     1261 tccggatttg tggggaattt cactgttaaa atagggataa aagcccgcag cgttaaggct
     1321 gacctttgca gcggttgtgg agtatgtaca gaaaaatgtc cgtcaagaaa agcaaaaagc
     1381 gaattcaata tgggcctgtc caacagagga gcaatttaca ttccctttgc ccaggcaatt
     1441 cccaatgttc ccgttattga ccgtgaacaa tgcattaagt tcaaaaccgg caagtgcgga
     1501 atctgcgaga agaactgctc tgcaggagct atcgacttta cacagcagga caaaatcgta
     1561 gaaaaacagt atggtgcaat tgtagttgca acgggcttta atcccatttc ccttaaaaat
     1621 tttgatgagt tttcatattc acggtgtcct gatgttgtga cctctcttga atttgaacgc
     1681 attatgaatg cagcaggtcc tacaaagggt aaactgctcc ggccgtcgga taatacacat
     1741 ccccgtacaa ttgttttcgt acagtgcgta ggttcccgcg atacatccgg ttgcggaaag
     1801 acatactgtt ccaaaatttg ctgcatgtat acagcaaagc acgctatgct tgtaagagag
     1861 aaatacccct atgtggatgt tcatgtattc tacatcgatg taagaacacc cggtaagaat
     1921 tttgatgaat tctatcgccg tgcagtggaa gaatacggcg tcaattatat aaaaggaatg
     1981 gtcggcaaaa ttgctgaaaa aaatggcagg ctaatggtcc aaggctctga tttgcttgca
     2041 gatgaacaga ttgtaattga agcagatatg gttgttcttg caacagccat tgaacctgac
     2101 aagactgcta aagatcttgc tactatgctg actgcgagca ttgatactga taatttcttt
     2161 actgaagctc atcctaaact tcggcctgta gaaagtccta cagcaggtat ttatctttcc
     2221 ggtgtatgcc aggggccgaa ggatatacct gagactgtgg cgcaggcgtc agcagcggct
     2281 gcaaaggtaa tcgggctcct tgctaaggat aaacttctat gcaacccatg tgtatccagt
     2341 tcagatgaga tgctctgcaa tggctgttcc tcatgtgaaa aggtttgccc atacggtgct
     2401 atatcctatg tagataaaga atttagagct gcaggaaaga ttgaagttcg cagagtcgct
     2461 caggtcaacg aggcagtttg tcagggctgt ggtgcttgta ctgttgcatg cccatcaagc
     2521 gctatggatt tgaaaggttt tacaaacaaa caggttatgg cggaggtgga tgcaatatgc
     2581 cgataaaaga tgagttcagg ccgttgattg ttgcattctg ttgcaactgg tgcagttatg
     2641 caggcgcaga ccttgcagga accagcaggc tttcataccc tgcagatgta aaaataatta
     2701 gagtgccttg ttccgggcga attaacccta tgtttattct tcgtgcattc caaagaggtg
     2761 ccgacggtgt tatcatctgc ggttgtcatc cgggagactg ccactattca actggcaact
     2821 actatgcaag aagaagaatg acactgcttt tcagtctgct tgattatctg ggaattgaac
     2881 gggaacgtac aagagttgac tgggtttcag cagcagaagg cgttaaattc tctacggtta
     2941 tgaacgagtt ttcaaagaca attcatcagc ttggtgaaaa taaaaggttg ggggatctga
     3001 gatgcaaaga atttttggat taattaagga taaaaccgaa aaactgcttg ctgacgggac
     3061 cgtcaacagg gttatcggct ggaaaaaagg tgagcttttt tatgataaca ctccggctgt
     3121 tttcaacata agtaccgtgc ctggcacgac gcttaccgga gatgagatgg aaagctttgt
     3181 gtatgatggc ttttgtgcaa gcaaccttag caagtacctt attagcgaaa gcaaaaaaga
     3241 cgggaaaata ttagcactgc ttaagccttg tgatacgtac tgctttaacc agctcgctaa
     3301 agaacataga attaacaaag acaatgttta tattctcggc attgagtgca gaggaatgat
     3361 agatattaac aaaatcaagg acttgggaat caaaggaatc ataagtatcg aagagaagga
     3421 ctatgagtat gagaaatatg atcaacttat aattcagaca atttatggtg agaaaacctg
     3481 taagaagagg gaagtattgc ttgaaaagtg cctttgctgc aaaggcaaac ggcatgtgac
     3541 ttacgatgag cttttgtcaa ggggcgatca tacccccgag gatactcgtg cagatattcg
     3601 cgtattgact ggcgagaata ctcctgaagt cactccggaa gtaacccatg aagatactag
     3661 caaagatatt cgcaaaggta ctcccgaagc aactcgcaaa aaagcttccc agcttgattc
     3721 gaatcgcttt gagcttgtgg aaaagcttga ggctatgact cccgatgagc gattttcatt
     3781 ttggcggggc gagctttcaa agtgtatccg ctgtaatgca tgcagaaatg tgtgccctgc
     3841 atgctcctgt ataaaatgtg tttttgataa cgataactca ggcattgcaa gcaaggcaaa
     3901 cactgatacc ttcgaagaaa acatgttcca tatcatccgt gcgttccatg tagcaggacg
     3961 ttgtactgat tgcggagagt gttctagagt ttgcccccaa cacatacccc ttcacctttt
     4021 aaatcgcaag attatcaagg atataaacga gttatatggt gaatatcagg ccggtgctga
     4081 tacggaatca cgtgcaccgc tggcagactt cactaaagaa gacgtggaac caagcattgt
     4141 agcgaaaaaa ggaggagagc actaatgttt aaaatatcaa ctcagtgttt gacagagctt
     4201 ttttccttaa tttccgataa cagagcactg tacatgccca ttgagaaaaa tggtcaggta
     4261 caatttggca agtggcaaag gaatgaaaaa gttcgccttg ataaactcaa tgcagtaaaa
     4321 tccccaaagg agtttttctt tccccagagc gaaaatattg tggctttcaa aaccaaagga
     4381 aagaatatct ccattataga taacagagat gaaagtgaag catttgtcgt tttcggtgtt
     4441 cgagcatgcg atgcagccag cttgggaatt ctagacaggg ttttccttgc agagcctgta
     4501 gacacatttt acaaaacaag acgggacaag ggcattataa tcaccctcgc atgcagtgaa
     4561 cccgaggaaa catgcttctg cagtgttttt ggaattgatg cagctaatcc ggcaggagat
     4621 gttgccacat ggattatagg tgatgtgctt tactggaaga gtcttacccc aaaaggcgaa
     4681 gcacttacag aaacggttaa gagcaagagt aagattaaca gcaacagtaa cagcaacagt
     4741 aacagcaaca gcaatagcaa cagtaacagc aacagcaata gcaacagtaa cagcaacagc
     4801 aacagcaaca gcaacggtaa cggtaacagc aacagtaaca gcatatttga aatggctgat
     4861 tcttctgacg aagaaaagct tgaaggttat aaagagaatg ttaaagaaat cattcaaaaa
     4921 cttccttttt atgatttgag tcttgatagc ttcaatagtg aagtattagg cgaagcatta
     4981 aaggataagt ttaattcgcc aaagtggaag gaattatccc aatcctgtat tggctgcggc
     5041 acatgcactt ttgtatgccc aacctgccaa tgctacgata ttagggatta tgatacagga
     5101 cacggcatcc agagattccg ctgctgggat tcctgcatgt attccgattt tacgaagatg
     5161 gcccacggca atcctagaac aagccagctt gaaagattca ggcagagatt tatgcataag
     5221 ctggtttact tccctgcaaa caatgatgga gtatattcct gcgtaggctg cggaagatgc
     5281 gttgctaaat gcccggtttc tatgaatatt gtgaaagtta ttaaagcaat gggggtgaag
     5341 gaaagtgtgc gactgtaatc acagtacagg tgaatgtaca tgtaaaggca caggtggaag
     5401 tacgggtgaa gtattaatcc cgaagctggg cgttgttact gatataaaaa tcgatacacc
     5461 cgatgtgaaa accttccggg taaatgctct tgacggtggt gtatgtttta agcacatgcc
     5521 cggtcagtgt gctatgctct caatccctgg cgttggtgag gctatgtttt caatcacatc
     5581 ttcaccaacc aataccgagt atatggagtt tagcataaag aaatgcggac atctcaccga
     5641 atggctgcat cagatagata tgggacagca gattacaatc agagggccat acggaaacgc
     5701 ctttcctatg gaaactgaac taaaaggcaa ggatttgctt tttatagcag gtggaatagg
     5761 acttgcgccg ctcaggtcgg ttatcaacta tgctcgccat tacagaaaaa actacggtca
     5821 tattgatata gtttatggtt caaggagtaa agatgatctg gtcaattatg atgaaattat
     5881 aaatgagtgg tgtaatgatg atggtattaa tgtctatttg acaattgacc gtgagcagga
     5941 aggctgggac ggtcatgtag gatttgtacc taattatata aaggaattga actttaatac
     6001 taataaaaca gcaattgttt gcggacctcc catcatgatt aaatttacgc tggtagcctt
     6061 gcaggaattg ggcttcgaca aaacacagat atttactaca atggagctta agatgaaatg
     6121 cggtgttggc aagtgcggac gctgcaatat tggttctaaa tatgtctgca aggatggtcc
     6181 ggtatttcgt tgtgatgagt tggacgaact accaaatgaa tattaatcaa aataaggaaa
     6241 atcagggaga aattcaggga cactcctcta cattagagac actttaggga cactcctcta
     6301 cattagagac acattaggga catttcacaa cataaagaac actccttaaa gtaaaaagaa
     6361 tgcctgatgc ttgagagagg agtgtatccc tattcctaaa gtgttcctat acctaaaaag
     6421 agaggagtgt ccctatacct tggatggata ccttggtata cctttagaga aaaagactca
     6481 acaagaaagg acgtgaagta atggaagtaa tggaagtaat ggaagaaatg gttaatgtat
     6541 ttttgatggg taaaaaatat gctgttcctt caaaccttac aatcatggga gcgatggagt
     6601 atgcaggata tcagcttgta cgcggctgcg gatgccgtaa tgggttttgc ggtgcttgcg
     6661 ctataatata tagaatcaaa ggcgaacgtg agcttaaagt ttgcctagca tgtcagacaa
     6721 aggttgaaga caacatgtat attgcaacac tgccgttttt cccgcttgtt aaagaggttt
     6781 ataatataaa tgaaataaag cccacagaac agattatgat gcagctttac cctgaaattt
     6841 acagttgtat tggctgcaat gcatgcacca agagttgtac acaggggctt gatgtcatgc
     6901 agtatattgc atatgcacaa agaggagaat acaaaaagtg cgcagaagca tcatttgact
     6961 gtgtaatgtg tggtgtatgc tcatcacgct gccctgcagg tatatcacat ccacaggtgg
     7021 cacttcttgc aagaagactg acaggaaaat atcttgcacc aaaatcccag caccttgaaa
     7081 acagggtttg tgaaattgga aatggtgatt ttaaggaact gctggaagga cttatgcaga
     7141 aacccattga gaaattaaaa gaactgtata acaatagaga aattgaagag taagggggga
     7201 tgattggatg aagatagctg ataggaagaa gatggatgat aggatgatga ctgataacag
     7261 gacgatgact gaaaacagga cgataactga taacaggacg ataactgata acaggacgat
     7321 gacggctaac aggacaatga ctgaaaacag gacgataacg gataacagga taattatgta
     7381 tacacaagat atgcttgaat caataagaaa agttgaagcc acaagaaaag aaagactcag
     7441 catggagcct gaaagaatga cagcagatga gaaagaagtg ctgctaaaaa agtatcatcc
     7501 cgattatagg caggagaact tttctgtctt gaaaattggt ccaaacaagg gtgacaaggt
     7561 tcctgatgag ctagctgatt tgcttcaggc aaacagccgc ataaaggatg tagacataga
     7621 cctttcaaag gttgactatg atattgatgt attaattatc ggcgggggcg gtgctggagc
     7681 atctgctgca atagaggcca acgaaggcgg cgctgacgta atgattgtta ccaagctccg
     7741 tatcggcgat gcaaacacta tgatggcaga agggggaata caggctgccg acaaggaaaa
     7801 cgattctcct atgattcact atcttgatgc acttggcggg ggacattacg caaacaagcc
     7861 cgaacttctt aataaacttg taacagaagg tccttcggca gttaagtggc taaatgacct
     7921 tggtgttatg tttgataaag cacctgatgg cacaatgata acaactcatg gcggaggaac
     7981 ttctcgcaag aggatgcatg ctgcaaaaga ctattccggt tcggaaatca tgcgtgtttt
     8041 aagagatgaa gttataaacc gtggtattac tgttgttgat ttcactgcgg ctgtagaact
     8101 tattcttgat gatgaaggca aggctgccgg tgccattttg atgaacatgg aaacaaagga
     8161 atatatggtt gcaagagcaa agacaattat tatcgcaaca ggcggtgcag gacggctgca
     8221 ttatcaggga ttccccactt ccaatcacta tggtgctaca gctgatggtc tgatattagg
     8281 ctatcgtgca ggtgcaaagc ttctttatgc cgacacgatt cagtatcacc cgactggtgc
     8341 tgcatttcct gagcagattt ttggagccct tgtgaccgaa aaggttcgtt ccctgggtgc
     8401 catgctggtc aacgctgatg gagaagcatt tatgcatccc cttgaaaccc gtgatgtttg
     8461 cgctgcatct attatcagag agtgcaagaa aagagaaaag ggagtatcaa ctctcgaagg
     8521 ctcaggtgta tggcttgata cgcccatgat tgaaataatt aacggtgaag gtacaataga
     8581 aaagagactt cccggtatgc ttcgcatgta taaaaaatat ggaattgata ttacaaaagt
     8641 accaattctt gtttatccta cgctgcatta tcagaacggt ggtttgcaga ttaatgcaga
     8701 aggacttact gcaattgata atctctatgc tgcaggagaa gtagtcggcg gcattcatgg
     8761 ccgaaaccgg ctcatgggaa actcacttct tgatattatt gtatttggca gaagtgcagg
     8821 taagaatgct gctaagaagt gcaagaatgt taaagtggga aagctgactc ttgaccatgt
     8881 aaataagtat gcaaaagaga tggaatcagc aggggtaaaa agtaattgtg cttcaccaaa
     8941 acttttaccg acatacaccc atagacaagt taaatgataa aaataatata aggttaataa
     9001 caataataca aggcgttttt ataaaatcat tatcgaatgg ccagtatatt tttaattatg
     9061 ccaaaaaata tattataata tataaaaata acgtacaatg aaaaggagac aataattatg
     9121 caaggattag ctagtgtttt agagacattt atgattgtga gtttcggagt ttcatggccg
     9181 atctccatta taaaatcaca ccgttcacgt tcaacaaaag ggaaaagcct gttttttatg
     9241 tgctttattc tttttggata tatttgtgga attgcgtcaa aatgtatttc caatacatac
     9301 aatcttgcat tttggttcta ttttccaaat gttattatgg tagccacaga catttgcctt
     9361 tatttcagaa ataagagact tgaagctaag tgaaaaggaa agtgagggac agtccttcac
     9421 tttctccact tctgttaaag tcttgaaatg taggggacag tccttcagga aaggtagggg
     9481 acagtccttc atatttgtta caaaaaaagt gggggactgt cccccacttt tttaccctca
     9541 ctttttcaat taaatctccg gtattacgat ttcaatttct cttccaagct ctgctgattt
     9601 agaaattcca tcaattattg cctggttata caatatctga cttgctggaa caggtgcact
     9661 agttccgtct attatagcat ccaagaaaga tcttaccttt aaattaaaaa gatcctcttt
     9721 cgttttaagt attggtactt ttgtctcagt ttgcaatcct gctacatcgt gatatatagt
     9781 catagccccg ccaacagacc cattccagca ttcggttgat ggaatacgaa gagctccttt
     9841 tttaccgaaa ataattgtgt ctcctgtggt atcaggatgc atggcccatg caattctaaa
     9901 atccaatata atgccgcctt ccagcctaat aaaagctgca gcaaaatcat ctactccaaa
     9961 tatatctgca tactctggtt tgtttgaata ttctttactt ttgccaaaga aatcagattt
    10021 ataaccagtg acagtgagtg gctttggata accgattgca tttaatacca tatcaagtga
    10081 ataacaacct atatcgccaa gtgcgccaat accagcagtt tccttttcta taaatgaagt
    10141 tccatatgga gtaggtattc ctcttcttct tccgccgcct gtttgaatat agtatatttc
    10201 accaagttca cctgattcaa ctattttctt tatcattttc atattttcat caaaacgggg
    10261 ttggaatcct atggaaagga ctttgccgct ttctttttct gctctcatta tttcaattgc
    10321 ttcatcaagg gtaacacaca taggtttttc aagtaaaaca ttcacacctt tttttagtgc
    10381 atatattgta cactcggcat gagttgcatt ataagtacat acactaactg catccagttc
    10441 ctcattatcc aacatttcct tatggcttag gtagactctt gctcctttaa catcatgatc
    10501 ttcaaaaaat ttcttggctt ttccctctat taaatcagca cctgcaacaa cttctacatc
    10561 cggcatattt ttatacgcta tcaaatgata ttctgcaatc catcctgtac cgattattcc
    10621 aacctttaat ttttttgaag tatccctgtt tttagtatca gaaatttttt ctttaaactt
    10681 gttcaatagt tcactattgg ccataatacc ctccattccg ccactactat ccgcggctaa
    10741 aatagtaata taaattattt ttcacgttaa acctttcagt tgaaattcca gttacaattc
    10801 tagttccagt tgaaattaaa gatccaactt gaatcccagt tcaaattcca gttcaaattc
    10861 cagttcgaat tccagttcga attccagttc gaattctagt ttcagttcaa attaaagatc
    10921 caattataat cctaaattct taataaattc gcagctaagt ttggcagatt caagttcggt
    10981 ttgaccttta gcaggtctat cctgctccac tataacccat tttgtattaa cttttttaca
    11041 agcatctaaa atacttttga aatcttgaac cccataacct acgggacgaa aagcaaaatc
    11101 ttcttttaca tcttcatcta tctctacaga tgaattagtc cctagaaatt catataaatt
    11161 ttcttttcta gctctgtttt tcattacaaa atcctttaaa tgcacaattg gagccctatc
    11221 ttgatacttc atcaaatatt ctgaaggatt tactcctgca acattaaccc agcaggtgtc
    11281 aatttgtgtt tgaagcaaat cttttgaaat agatttgtaa attgtatcca agtaatattc
    11341 tccatctaca atatcaaact caaaattgtg attgtgatat agcattgtga taccatattc
    11401 tttagctttc tcgcaaataa aagttatgtt tttcaatgtt gtatcaaaat ttcctgcttg
    11461 tggtctatct tcttccggta aatgtggaat agcaatgtac tcaagtccaa tatctttata
    11521 tgtttgaaat gttttatcag gctcttccag caattcgtct aaaggaacat gagctgatac
    11581 agcctttaaa gaaagctcat ccagcatctt tttaatttcc ttgcctttct ttccaaaaag
    11641 cccggctaac tctactcctt catatcccat ttcttttact ttttccaatg tgcccttaaa
    11701 atctttttcc atttcgtttc ttaaagtgta tagttgtaaa ccgattgata atggcataga
    11761 aatacccccc tttaagatat attctgattg tattataaga cgcaatatgc tatgatacaa
    11821 gtcaataagg tataattata ggacatatat tgtgcaatat gtaaatgaac aacagataag
    11881 gtgtaaagaa ataaggagta aagaaataag gaacatgaag ttaatagtaa agaataaaga
    11941 acatagaata tagaataaag tgaaaagtaa agtgtaagga gcaataacat ggttgccttt
    12001 tacgaaaata aagataaaga tttaacattt aactatggaa gcagactgga ctttcgtgct
    12061 catctacata aacatattga acttgtttat atgctagaag gtgaaaccat tacctatgta
    12121 gacgggaaga aagcaatttt aaaagaaggt gatgtattta tttcttttcc aaatcagatt
    12181 catcaatatt ttagtaaagg tcctgagaag tatttaatta ttatattttc accagatata
    12241 ctgccggaaa tgaagaatat atttataaac tatataccag aatcgcaagt tatagaaaag
    12301 gcatataagt gcccctattt atatacaatt ctatcttctg tagtaaaagc atacgaatcc
    12361 gaatacgaat atagaggcct gcaggtaaag ggatatttgc tgacattttt tggtgaactt
    12421 ttaaaaaaca tgaaactcac accaataaaa gcttcaaatt tatcaatgct tcaaagcata
    12481 ttaaattatt gctcagaaaa ttatatgaaa gatttatctt tagaatctat ttcagatgca
    12541 ctgcacgtaa gcaaatacta tgtttctcat ttgcttaaga aaaaaatcaa cataggattt
    12601 agtgactata tcaattccct tcgtatttct gcagcatgtg accatctatt atctgaagaa
    12661 aaaagtatca ctgaaatagc atattttgtt ggatttaata ccacaagaac ttttaacaga
    12721 gcttttataa aacatacggg acagacacca acttatttta gagcctccat gtcaggtgca
    12781 aaatgcacta taatgaataa atacatgtag ttatgacacg tacatgttac atgttatcgt
    12841 tttaacccaa atgttcaagc aaaatatgcc cactgttgct atgtgctatt tataccccaa
    12901 attagccatt cggacgatgc gcaattcaaa attctgtctt atagtacata gtatggtcta
    12961 tagtatattc tattctattc tatagtatat agtatatata ggatgggatt tttttacggc
    13021 ctgcgaagaa aggggtatgt gtgtgagtta tattaaaact gtactgccca tggagggcta
    13081 tcggctgctt atggagaggg gaaatggcag cactgttata gcagaccttt ccagcaaact
    13141 gcataccatg aaatatgcag atcttgcaga taaagtattc ttgataaccg ccacaactga
    13201 aggatgacta taattccgtg cctgaagata ctgttatgtt agtagcaaga caaaaattat
    13261 ttaaagcgga tatatgtaat ccttggaaac ttggttatga gagttcagat gcggacttat
    13321 tatatgcatt atatgcagat ccaatattat atgcagatcc aataaaacga tgggagagac
    13381 ctacctatga agagagtaga aatatgaaga gagtggaaat atgaagagag tagaaatagt
    13441 gaaattatat tcacggatga ttatggcaac cagcttgagc cggaagtagt gtttagcttt
    13501 aaagaaaaag tgggggacta gaaaagtggg ggagaaaagt gggggacagt cccccacttt
    13561 tctccccttc acttttctgt ccctcacttt tctcccattt tttcatagct ctctcactaa
    13621 cagcacacct gctataccga tcacaataag tgtcagtgaa taaagggcga ttgaggtgat
    13681 aaccagtgcc caaatccagc ccgctggttc gattgctcct gatgtgagcc cgctgattac
    13741 ggcaatagcc tgaccgccaa caagcaatgc taccatagta actaaacccc aggtgatgga
    13801 tttccaatgt gaatgcgccc gttttgcagc ccatatgagc agcagagcac caatcaggac
    13861 aaaataggaa agagctccgc cggcatcaga tagtcgaaca gtagaatgcg cgcagatatt
    13921 gttccgatta cagaggtcag gatggtaaac aggattggca gccatacaag gactgttcct
    13981 gctacagcta agatcttatt gaaaatatct ttattcctca ttctattata acctcctcag
    14041 catttaaaaa ataatcatac aaagcaatta ctcaaagcag tcacctaaaa caattaccca
    14101 aagcaatcac tcaagacgac aatacattcc atgacgtttc ttgtttctat tatatcactt
    14161 atctccacca cttatatttt ctacaacaag aggaatagta ccatatgtta tcatatgtta
    14221 ttgccttgat tgtatccata ttaaatcgtg ggtgcattta atttacttga agaaaatatt
    14281 ggtaaggggc ataagtgaaa gtgctgtagc caggcagggg aaacttttca ctcaagtaac
    14341 aggtattcct tttatgctaa agagtttccc cgtatctgag cgaaggaaga gcaaaatcag
    14401 tgtttgaaca aaataattac aattctttta ttcagatt
//
LOCUS       DAOSDD010000101        14354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1667_length_14354_cov_6.837961, whole genome shotgun sequence.
ACCESSION   DAOSDD010000101 DAOSDD010000000
VERSION     DAOSDD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1667_length_14354_cov_6.837961"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>8914)
                     /locus_tag="PK733_10265"
     CDS             complement(<1..>8914)
                     /locus_tag="PK733_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ10961.1"
                     /translation="GSTISWESDKDTIVDHTGNVTRPPYTSRDEIVTLTATISNGSYI
                     TTKAFTVTVIKLEQTNAEKLAADDLWLTEALILKENSALDNITGNLYLASLGPNGSNI
                     SWSSSDPLVVDTNGTVERQPHACGDQTVILTAIISNGSSITRTFTVTVKALEQTDAEI
                     LEADKEWLTELQILNGNTSIDNIMGNLSLPVEGPNGSTISWESDKNTVVDTEGKVIRP
                     AFTHGDESVTLTATISKGSFTATKSFTAIVKALEQTDMEAVNDALPWLTDYMTYINGP
                     LDNVIYNIKLPITGPDGVTINWASDNTAVVDSTGKVTRPTFTSGDKEVTLTATISKGA
                     SATAEKAFTVIVKKLPQNAQEKVEADAVWLTDAQILNGNSALDNITNDLTLPTLGENG
                     SNITWYSVYPNVVSAEGKVTRSGFTAGSKTVTITATITMGSGTFARKTFVLTVKPLDI
                     TDREAVFLDEQWLYRYNILALNPTVNSVTRNLSHPGSGPNNSFITWVSDTPTVISDDG
                     TVTRPEHGEGHKSVKVIATLTKGESQTERTFTYVVLEKPDTTPPAVVGTTPENNSTGV
                     SWDINTIAVTFNENIVRGSAADGSLQTFGIALQASGTVRFDAAIEENKLKITPYGDLS
                     PGENRLIIPAGAVTDAARNPAEAYELNFTVEPKPTRKIAVVSTTPQDLEKEVDINIKE
                     IEIRFDSSNLVLGEEYYMMMLRDKRHGFMRSNVGSLTGDIATLKVFGPLDPGTVYEIV
                     IPAGVVRDRFDNINEEKVIQFRTKYAAGNPLVISTNPSNGQTGVNVNQAEIEVFFSQE
                     VKPFYHQFTLNDDKGNQYVLTGYTTPDKNKLRLVPNAAKQFRPNTVYTLSGQYGTMEN
                     PSQMEFNCQFTTSTNELVLESTTPVAWKREVPLDAVVEVMFSAPCEKGSSFADITIKD
                     SEGVSVEFTGQEAGKKVILTPASMLSPSKSYTVTIPEEAFKGERDTTNDPYQFTFSTV
                     NKLDLNLRNIHIPDMGFVNKHAMFDASEAENIARRTGYKIVSYEWSVDGEAAGTKSYI
                     YHLFRSAGSHEVELTIKDNKGISYVFKKNIEIQNLTDIRMTIKDSELFPRSVIAEEIG
                     SQPGLVYELKLLQEGQFIYGERISVELFKDGVRQRALNVISAQYGEDVYKFTFKPQYG
                     EHGIYELEFTYKGIGYNQVIRIPVNVISGKPAIAEEFKFRLYDVDTAGYYEAPESLNV
                     FLNGKKMHAVKKWYPDKNYYAYTIVNESIYANTYYEFQVEAWISGNIHKNVYVGKDTS
                     DPFVIYGRSIKPEGIKNVSINYSESTVKDPYFRDLYFEGVSAKLVLNVEGDWKNAEEG
                     YYEIKTWKDDKFILKVNCNNRDGIQKITLDPGLQLKAGDTLIIRMVTKYGVASIWYYC
                     PYIHVITQPSFLGKELPISIQNGEYAVNWPTVFGGALGGTIGILDGIPVVGGGNFGIG
                     SDMPKFEGYLTGNVVNPYIDLEFGISGGYGEKSKTKADTKYKKLKKVTVVGYEFEISV
                     EGELNFIYNSETKEWSMYNFYIWIIGDGTKEWSKEYEFMGIGFSAGVGIGAEVYGGLK
                     IDNSKSKTQYSGIIGIAPHAYLRVSGDFVLARVDGYLNARLPAEIHFPTGYIGADITA
                     NAEIYAKALSWGDYIYKKDLYKVHWDNGKEKVILGSLQEQMRDFACKENTSLQLIPRD
                     YSNRQSSWIAVDRAIVPEEQIAVLASLGGLGLAGTWKAAAETNPAVSVMMENIYPHAE
                     TQLVRNQDELWLVWSDDNPARDAVNRTQLRYSVLKDDGMWSEPVWIGDDGTADFTPAV
                     AAAGNGALMAWHNIRKSVTEEEGLAGMLENSEISVTESVYTADGISPNIITLTNDDFI
                     DHSPKLAADGDKALLVWTKSESMGFSIGDEEVSESEKGNQLYFSAWNSGTWSAPESIG
                     DASSTVLDSSLAMGGDKGLLLYTLDMDNDLFTFEDREVFARLYEEEAWSEAIQLTSNT
                     FDDSAPKAVYADGQWFITWLQEGDILYKVGLNGETKAEERLKNIQGDYQLAAKSGTRP
                     LISLVYMQPGENKAVGISSSFYDLDNGQWGADVSLAAGNKYADAISVVFTEDGKLKVA
                     FSQAEIITEAVPMTVDDVEQLVEQPNISNKVDMKLLTYTPVHDVALLKEDGIFLSKEF
                     PLPGTVTTVYATLRNEGDFAENARIRIYDGNPDAGGELIGEAPSQLIPARSSEEVEIA
                     WLVGPEERDTYDLYAVVQPSEGVQEINTDNNTLNLEFSTADLSITGLTCENMAKDDYL
                     VTVTIANSGGKTLEGATVVMENVNDGRILETTSLKPIEPGQQTVLTTMVSSTGLARDV
                     KGQINLRICVNPPEGVKEDFTENNIRSFSLEPAAITVDRVNPGPGDKHVVPSSALNIT
                     FNMNIAQDAGYDRIRLVDEDLNEIAANKVIQGNTLTITPKHVLDNGTEYTLTIPADAL
                     GDAYGHAMKNDFSLSFMTTLTNPEIISAYPGEFMENTALDTDIRLKFNQNILKGNNFS
                     DISLFGPEDRMIPVNAAIEGEFLTLDYNGSLSENTTYSLEVPRGAVQNDNGEVLYEDY
                     VLVFTTDDANEGDDEPDGDTNEGDDEPDGDTNEGDDEPGGDANEDGDEPGGDANEGDD
                     RPGGEENDDDSPFSPDNTSNHEYQARLDIGGTKKTIEVHTEAGKAVIKLGTLAAEIFN
                     GNQNAILNIPAVPGTNTYMLEMPAETLASGNSSLTVNTVLGSVDIPSGMMSTLENIGG
                     KTFGITIAAVDKSTLPADVRAAIGNRPILQITLTINGVETAWSNPAAPVRVSIPYTPT
                     TAELESPESIIIWYIDANGNIITIPNGIYDRHAGEVSFRITHFSDFAVAYNKVVFKDV
                     PDDVWYDRAVSFIAARGITNGTGKGNFSPDNRLTRGEFITMMMRAYGIEPDTSPEDNF
                     SDAGNTWYTGYLAAAKRLGISKGVGNNMYAPDKDITRQEMFTLLYNALGVINQLPETK
                     TSDGANLGRTLSDFSDTGEIASWAREA"
     assembly_gap    8915..9014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9015..10444)
                     /locus_tag="PK733_10270"
     CDS             complement(<9015..10444)
                     /locus_tag="PK733_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10962.1"
                     /translation="MKVHRKIISIALTFIFVFTGLPVPFTNFDGIPDVYAAESTPSVD
                     ISYDNFFNTENLQLNGGSLIENNAIQFESGGDTGESVFTTDKVTLGENFSFSTAFSFR
                     NISPSTPAAGTKGGFTFTLQSVGNSAVASDFQDESIYPSLSIAFISNYMEAGATASIM
                     KQTENLHLASLTNVTLAEGPMNLCDIFAVPYLDGDYNNPISHYPINTYYVVDETSDYY
                     HVWIGYDGEEELLHFLCLDPLGQYKYFCDSVKLADIMLPNEVYAGFMGSLGNAGNASE
                     ISSWYFKNDLSIMDEALADADEAELTFLMEILEADPISYYYLPLTGQYGSTISWVSSN
                     TDLVAEDGTVNPPSLEQGNQTVILTATITKGSAERTTRSFTITVKVPDLDIVTADYDW
                     LTNSMILNENDALDNIVSDLLLRTSGQYGSTISWSSTNAVVADWDGTVSRPVYYDGDK
                     EVTLTAYITMNSVTLDKSFNITVKALG"
     gene            10632..11255
                     /locus_tag="PK733_10275"
     CDS             10632..11255
                     /locus_tag="PK733_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ10963.1"
                     /translation="MMKKKNMSKRKLQAAETKRKIFEAARQLIIEHGIENVSVDSIVK
                     AAGVSKGAFYVHFESKDALAAAFVNDYTNIADMDYKSFLASLSEHKSALDILVLLAEK
                     ISDFIDINIGFENMRVLYKAHLTKTINTASAMSYNRELYKLFTDALEKGVHQGEIRED
                     IPVDSLAKHLILAIRGITYEWCIRYPELSLKEQVLDHFKILLYGLKR"
     gene            complement(11543..11791)
                     /locus_tag="PK733_10280"
                     /pseudo
     CDS             complement(11543..11791)
                     /locus_tag="PK733_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            11997..12308
                     /locus_tag="PK733_10285"
     CDS             11997..12308
                     /locus_tag="PK733_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ10964.1"
                     /translation="MGTLYVEHAKVFKALCDENRLRILDLLRGDEKCACVLLEQLDLG
                     QSGLSYHMKILVESGIVESRQEGKWTHYKISEKGSTYAVALLKELTAPNTVIEENNCC
                     K"
     gene            12399..13412
                     /locus_tag="PK733_10290"
     CDS             12399..13412
                     /locus_tag="PK733_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ10965.1"
                     /translation="MQVLKTGWGFFQNQILGMKWLNDLIGSVLSVLGLDTSNRWIGSI
                     QFFIYDVIKIVVLLCVLIFIISYIQSYFPPERSKKILGRFHGIGANSVAALLGTVTPF
                     CSCSSIPLFIGFSSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGTKVAILYVIFGLA
                     IAIIGGTLIEKAHMEKHVESFVLTAGSVDIESPNLAKKDRLIYANEQTLSTFKKVFPY
                     ILIGVGIGAIIHNWIPEEWVVSLLGSNNPFGVILATVMGVPMYADIFGTIPIAEALLF
                     KGAQLGTVLSFMMGITTLSLPSIIMLRKAVKPKLLALFITVCTVGIILVGYLFHFLQQ
                     ITI"
     gene            13542..13895
                     /locus_tag="PK733_10295"
     CDS             13542..13895
                     /locus_tag="PK733_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH895/ArsE family thioredoxin-like protein"
                     /protein_id="HOJ10966.1"
                     /translation="MAFLNFGKKKGKTPPCCCGGSCDTESMAKAEKAKAEGASVKVLG
                     SGCAKCNQLEGATKAALEQLGMDATIDHVTDFAQIAAYGVMTTPALVVDGKVVSYGKV
                     LKTDEVVKILQKVRV"
     gene            complement(13885..14163)
                     /locus_tag="PK733_10300"
     CDS             complement(13885..14163)
                     /locus_tag="PK733_10300"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ10967.1"
                     /translation="MLTGKAEVSYDDEKRKEFWNDGMKKYYPLGYTDPGCMASSELVR
                     YQPDFALIRFTASKGSYYHGWAILYRYYQVFFRKANSCIFAFFYIFIL"
     gene            complement(14163..>14354)
                     /locus_tag="PK733_10305"
     CDS             complement(14163..>14354)
                     /locus_tag="PK733_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10968.1"
                     /translation="FLEQHFSYSNNDKGFVVYGPPNKSKSGIKEFWFCSNTSSKRVVQ
                     IQKTLMPAFISMMRKPSRV"
BASE COUNT         3790 a         3289 c         2866 g         4309 t
ORIGIN      
        1 ttgcctctct tgcccaggaa gctatttctc ccgtgtccga gaagtcagaa agggttctgc
       61 ccaggttggc tccatcagat gtttttgttt caggtaactg atttataact cccaacgcgt
      121 tatacagcag tgtaaacatt tcctgacggg tgatatcttt atccggagca tacatgttgt
      181 tccccactcc tttagatata ccaagccgct ttgctgctgc caggtagcct gtataccagg
      241 tgtttccggc atctgagaag ttatcctccg gactggtgtc cggctctatg ccgtaagctc
      301 tcatcatcat ggtgatgaac tctcctctgg tcaacctgtt atcagggctg aaattgcctt
      361 tcccggtacc attggtgatt ccccttgcag cgataaagct tacagccctg tcataccaga
      421 catcgtccgg tacatcctta aagactacct tgttataagc tactgcaaaa tcactgaaat
      481 gtgtgattct gaagctgact tccccggcgt gccggtcgta aattccgttt ggtatggtta
      541 tgatattacc attagcatcg atataccaaa tgattatact ttccggactt tccagctctg
      601 ctgtcgttgg tgtgtatggg attgataccc ttacaggggc agccggatta ctccaggcag
      661 tttctacgcc gttgatcgtc agagtaatct gtagaatcgg cctgtttcca atagcagctc
      721 tcacatctgc gggcagtgtg gacttgtcaa cggccgcaat ggttatacca aaggttttac
      781 cgccaatatt ctccaacgtc gacatcatgc cggatggaat atcgacgctg cccaaaacag
      841 tatttacagt taaggaagaa ttgcctgagg caagggtttc agctggcatt tccagcatat
      901 aggtatttgt accgggaacg gcaggaatat ttaggatagc attttggttc ccattaaata
      961 tctctgcggc cagggttccc agttttataa cggctttacc ggcttctgtg tgtacttcta
     1021 ttgtcttttt tgtgccaccg atatcgagcc ttgcctgata ttcatgatta ctggtattat
     1081 caggagagaa tgggctatcg tcatcatttt cttcaccacc aggcctatca tctccttcat
     1141 ttgcgtcgcc gccaggctca tcgccgtctt catttgcatc accgccaggt tcatcatctc
     1201 cttcatttgt gtcgccgtca ggctcatcat ctccttcatt tgtgtcgccg tcaggctcat
     1261 catctccttc atttgcgtcg tctgtggtga ataccagtac ataatcttca tacagtactt
     1321 ctccgttgtc attttgcact gctcctctgg gtacttcgag agagtaagtg gtgttttcac
     1381 taaggcttcc gttgtaatcc aaagtcagga actccccttc tatggctgcg ttaacaggga
     1441 tcatcctgtc ttcaggccca aacagggaaa tgtctgaaaa attgttcccc ttgagtatat
     1501 tctggttgaa tttcaatcgg atatcggtgt ccaatgccgt attttccatg aactctccag
     1561 gatatgcaga gatgatctcc ggattggtta aagttgtcat aaaactcaaa ctaaagtcgt
     1621 tcttcattgc atgtccatag gcatcaccca gagcatcggc gggtatggtc agggtatatt
     1681 cggtcccatt atccagaaca tgcttaggtg ttatggttaa ggtatttccc tgaatcactt
     1741 tatttgccgc aatttcattt aaatcttcat ccaccagcct tatgcggtca tatcctgcat
     1801 cttgtgcaat attcatgtta aatgtgatgt tcaaggcact ggatggaaca acgtgcttgt
     1861 cacccgggcc ggggtttacc ctgtctacag taatcgctgc cggttccaaa gaaaagctgc
     1921 ggatattgtt ctcggtaaaa tcttccttta ccccttccgg cgggttgaca caaatacgca
     1981 ggttgatttg tccctttaca tcccttgcca gccccgttga agaaaccata gttgtcaaaa
     2041 cggtctgctg cccgggttct atgggtttaa gtgatgtagt ctccaatatc cggccatcgt
     2101 taacattctc catcaccacc gtggctccct ccagtgtctt gcctccactg tttgcgatgg
     2161 ttaccgtcac cagataatca tcttttgcca tgttttcaca ggtcagaccc gtaatggaaa
     2221 gatccgcagt tgaaaactcc aaattgaggg tgttattatc ggtatttatc tcctgtacac
     2281 cttcactggg ctgaaccact gcatataaat catatgtatc cctttcttcc gggccaacca
     2341 gccacgctat ctccacctct tcggatgagc gtgcgggtat aagctgcgaa ggtgcctcac
     2401 cgatgagttc tcctcctgca tcggggtttc cgtcatagat gcggatcctt gcattctccg
     2461 caaaatcccc ttcattccga agggttgcat ataccgttgt caccgtaccg ggcaacggga
     2521 attctttcga caggaaaatg ccatcctcct tcaaaagggc tacatcatgc accggtgtgt
     2581 aagtcagcag cttcatatcc accttgttgc tgatattcgg ttgctccacc aactgctcaa
     2641 catcatcaac tgtcatcggt accgcttcgg ttatgatttc tgcctgtgaa aaagccacct
     2701 tcagctttcc gtcttcggta aacaccacgc tgatggcatc ggcgtactta ttcccggctg
     2761 ccagtgaaac atcggcaccc cattgcccat tgtccaaatc gtagaaggaa gatgaaatgc
     2821 ccactgcctt attttcacct ggctgcatgt acaccagcga aatcagcggt cttgtacctg
     2881 atttagcggc aagctgataa tcaccttgaa tgttcttcag ccgttcctct gcttttgtct
     2941 ccccgttcaa accaaccttg tacagaatgt ccccctcctg cagccatgta atgaaccatt
     3001 gtccatctgc ataaactgcc tttggagcag aatcatcgaa agtattactg gtaagctgaa
     3061 ttgcttcact ccaggcttct tcttcataaa gccttgcaaa gacctcccgg tcttcaaagg
     3121 tgaagagatc gttatccata tccagggtat aaagcagcaa gcctttgtct cctcccatgg
     3181 ccaggctgga atcaagcact gtagaagacg catccccgat ggattcgggt gcactccatg
     3241 tgccgctgtt ccatgcggaa aagtataatt ggttaccttt ctctgattcc gaaacttctt
     3301 catcaccaat actaaagccc attgactcgg acttagtcca taccagcagt gctttgtcac
     3361 cgtctgctgc aagttttggt gagtggtcta tgaaatcatc atttgtcaga gttataatat
     3421 tgggacttat gccatccgct gtataaacac tttcagtaac actgatttcg gaattttcaa
     3481 gcattcctgc aaggccttct tcttcggtta ctgattttct gatattatgc cacgccatga
     3541 gggctccgtt tccggcagct gccacagcag gggtaaaatc ggctgttccg tcatcaccaa
     3601 tccacacagg ttcactccac ataccatcat cctttaagac agaataacga agctgtgtcc
     3661 ggtttaccgc atccctagcg ggattgtcat cagaccacac cagccataac tcatcctggt
     3721 ttctgaccaa ctgtgtttct gcgtgaggat atatattttc catcattaca ctgacagccg
     3781 gattagtctc tgctgcagcc ttccaagttc cagccagacc taagcctcct agagaagcca
     3841 ggacagctat ctgctcttcc ggtacaattg ctctatccac agctatccaa ctggactgcc
     3901 tgttgctgta gtctctaggt atcaactgga gagacgtatt ttctttacag gcaaaatctc
     3961 tcatctgctc ctgcaaggag cctaaaataa ccttttcctt tccattgtcc caatgcacct
     4021 tatagagatc ttttttatat atgtagtccc cccacgataa cgcttttgcg tatatttcgg
     4081 cattggcagt gatatctgcc ccgatatagc ccgttgggaa atgtatttcc gccggaagac
     4141 gtgcgttcaa atacccatcc accctggcca gtacaaagtc tcccgatacc ctgagatagg
     4201 catgaggagc aatccctata attccactgt actgggtttt acttttacta ttatcaatct
     4261 tcaagcctcc atacacttca gctccaatgc ctactcctgc tgaaaatcca atgcccatga
     4321 attcatactc cttggaccac tcctttgtac catccccaat aatccaaata taaaagttat
     4381 acatgctcca ctctttagtt tctgaattat aaataaagtt taattcgccc tcaaccgata
     4441 tttcaaattc atagcccacc acagtgactt tcttcagttt tttgtatttt gtgtctgctt
     4501 ttgtcttgct cttttctcca tagcctccgc ttattccaaa ttcaagatcg atataaggat
     4561 taacaacgtt cccagtaaga tatccctcaa atttaggcat atcgctgcca ataccgaagt
     4621 ttcccccgcc tacgacaggg atgccatcaa gaataccaat ggttcctccc aaagcaccgc
     4681 caaacactgt aggccagttt actgcatatt ctccgttctg aatcgaaatt ggcagttctt
     4741 ttcccaaaaa gctcggctga gtaataacat gaatataagg gcaataatac cagatggacg
     4801 caactccata ttttgtcacc atacgaataa ttaacgtatc tccggcttta agctgaagac
     4861 cgggatcaag cgtgattttc tgaatcccat ctctgttatt gcagtttact ttcaggataa
     4921 atttgtcatc cttccaggtt ttgatttcat aatatccctc ttcagcgttc ttccaatccc
     4981 cctccacgtt taataccagt ttggccgaca caccttcaaa atatagatct ctgaaataag
     5041 gatccttaac agtgctttca gaataattga tgcttacgtt ttttatacct tctggtttaa
     5101 tggatcttcc atatattacg aagggatcgg aagtatcctt tcccacataa acatttttat
     5161 gaatgttccc gcttatccag gcctccacct gaaattcata atatgtattg gcataaatag
     5221 actcatttac tatggtatag gcataataat ttttatccgg gtaccatttt ttaaccgcat
     5281 gcatcttttt accatttaaa aacacattaa gcgattccgg tgcctcataa tagcctgccg
     5341 tatctacgtc atacaatctg aatttgaact cctcggctat ggcaggttta ccggatatga
     5401 catttaccgg tatgcggata acctgattat accctatgcc tttataggta aactccagct
     5461 catagatacc atgttccccg tattgaggtt taaatgtaaa tttatagaca tcctctccgt
     5521 attgagcgct gattacattt aatgctcgtt gtcggacacc atccttgaac agctctaccg
     5581 aaatcctttc accgtatata aactgaccct cctgtagcag tttcagctcg taaacaaggc
     5641 cgggctgact gccgatttcc tctgcaatta cgctacgggg aaataattcg ctgtctttta
     5701 tggtcatcct tatgtctgtc aaattctgaa tttcaatgtt ctttttgaat acataggaaa
     5761 tgcccttatt atccttaatg gttaattcca cctcatggga tcctgcagag cggaataaat
     5821 ggtagatata actcttagta cctgcagctt caccatcaac gctccattca taggatacaa
     5881 tcttgtaacc tgttcttctg gcaatgttct cagcttcgga cgcatcaaac atggcatgct
     5941 tgtttacaaa ccccatatcg ggtatatgta tgttgcgcag gtttaaatca agcttattta
     6001 cagtagaaaa tgtaaactga tatgggtcat tcgtggtatc cctctcacct ttaaaggctt
     6061 cctcggggat tgttaccgta tagctctttg atggtgaaag catggaagca ggtgttaaaa
     6121 ttactttttt ccctgcttcc tgcccggtga actccacaga aaccccttca gaatctttta
     6181 tggtgatatc tgcaaaagaa gagccttttt cacaaggggc actaaacatg acttccacaa
     6241 cagcatcaag aggaacttct ctcttccatg caacaggagt tgtgctttcc aataccagct
     6301 catttgtact ggttgtaaat tggcaattga actccatctg ggatggattt tccatggtac
     6361 catactgacc ggataaggta tacaccgtat tgggccggaa ctgtttggca gcgttcggca
     6421 ctaacctcag tttattttta tcgggtgtgg tataccctgt taagacatat tgatttccct
     6481 tatcatcgtt aagcgtaaac tgatgataga agggttttac ttcctgtgaa aaaaacactt
     6541 ctatttcagc ctggtttaca ttcacaccag tctgtccatt ggaaggattt gtactgataa
     6601 ccagcggatt tcctgcagca tattttgttc tgaactgaat aaccttttct tcgtttatat
     6661 tatcaaacct atcccggaca acacctgccg gtatgacaat ttcatatacc gttccggggt
     6721 caagaggtcc gaataccttc agagtcgcta tatctcctgt cagtgaaccc acgttcgacc
     6781 tcatgaatcc atgccttttg tccctcagca tcatcatgta atattcttca cccagcacca
     6841 aattgcttga atcgaaacga atttctattt ctttgatatt aatatccact tctttttcaa
     6901 gatcctgcgg tgttgtggaa acaacggcta tttttctggt tggtttcggt tcaacagtaa
     6961 aattcaattc ataagcctct gccggatttc tcgccgcgtc ggtaacagcg cctgcaggta
     7021 tgataagcct gttttctccc gggcttaagt caccataggg tgttatcttc agcttatttt
     7081 cttcaattgc agcatcaaat cgtacagtgc cggatgcctg aagcgctatg ccgaaggttt
     7141 gcaggctacc atcggcagcc gatccccgga caatattctc gttgaaagtt acggcaatcg
     7201 tattgatatc ccatgaaact cctgtgctgt tattctccgg cgttgtaccc actactgcag
     7261 gaggagtcgt atcaggtttt tctaaaacaa cataggtgaa agttctttca gtttgagact
     7321 cacccttagt cagagtggca ataactttta cagatttatg tccttcccca tgttcgggtc
     7381 gggtaactgt gccatcatcg gaaatcaccg taggggtatc agatacccag gtgatgaagg
     7441 aattgtttgg accagagcca ggatgggaaa gatttcgtgt cacagaattc accgtaggat
     7501 tcagagccaa tatattgtac ctgtaaagcc attgttcatc taaaaatacg gcttcccgat
     7561 cggttatatc cagcggcttc acagtaagta caaaagtttt ccttgcaaag gtaccggaac
     7621 ccatggtaat ggttgccgtg atggtaactg tcttactgcc tgcggtgaat cctgaccttg
     7681 tcactttccc ttcggcagat accacattgg gatatacgga ataccaggtg atgttacttc
     7741 cattttcacc gagggtcgga agcgttaaat cgtttgtaat attatccaaa gcactgttcc
     7801 cgttaaggat ttgtgcatcg gtcagccata cggcatctgc ttccactttt tcctgggcat
     7861 tttgaggaag ttttttaaca ataaccgtga aagccttctc ggcagtagct gaagcaccct
     7921 tgctgatggt tgcagttagc gtaacctcct tatcgccgga agtaaaggtc ggccttgtta
     7981 ccttgccggt gctgtctaca acagcagtat tatcggatgc ccagttgatg gtgacaccat
     8041 ctggcccggt gatcggaagt tttatattat agataacatt atccaaagga ccattgatat
     8101 acgtcatata gtctgtcagc cagggaagag cgtcattcac tgcttccata tctgtttgtt
     8161 caagggcttt tacgattgct gtgaatgatt ttgttgcggt aaacgagccc ttgctaatgg
     8221 ttgcagtcag ggttacgctc tcgtctccat gcgtgaatgc cggccttatg accttgcctt
     8281 cggtatccac aactgtattt ttatcagatt cccaactgat ggtgcttcca ttagggcctt
     8341 ctacaggcag gctcagattc cccatgatat tatctataga agtgttgcca tttagaattt
     8401 gaagttcggt cagccattct ttatctgcct cgagaatttc ggcatcggtt tgctcaagtg
     8461 cctttactgt gactgtgaag gttctggtga tggatgaacc gttactgatg attgctgtga
     8521 gaattactgt ttgatcgcca caagcgtgag gctgtctttc tacagtacca ttggtgtcca
     8581 caaccaaggg atcggaagat gaccagctga tgttgctccc gtttggccca aggctggcaa
     8641 ggtataaatt ccctgttata ttgtccaagg cactgttttc tttcagtatt agtgcttcag
     8701 tcaaccataa atcatcagca gctaactttt ccgcattggt ttgttcaagc tttattactg
     8761 ttactgtgaa tgcctttgtt gtaatatacg agccattgct gattgtcgct gtcagggtta
     8821 cgatctcgtc tctgctcgta tatggcggtc ttgttacatt acctgtatga tctacaatcg
     8881 tatccttatc cgactcccaa ctgatggtgc ttccnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnncccaat gcttttacgg tgatattgaa tgacttgtcc agtgtcaccg
     9061 aattcatagt gatatacgca gtaagggtaa cctccttgtc gccatcataa tatacaggcc
     9121 tggacaccgt tccgtcccaa tccgctacga cagcattagt ggaagaccaa ctgatggtgc
     9181 ttccgtattg accggaggtg cgaagaagta aatccgatac aatattgtcc aaagcatcat
     9241 tctcgtttag tatcattgaa ttggtcagcc agtcataatc ggcagttact atatccaaat
     9301 ccggcacttt aactgtaatt gtaaaagatc ttgttgtacg ctctgcagac ccttttgtta
     9361 tcgttgcagt cagtatcacc gtctggttcc cttgttccag ggagggtgga ttcaccgttc
     9421 catcctcagc caccaaatct gtattgctgg agacccagct gatggtactt ccgtattggc
     9481 cagtaagagg aagatagtaa taagaaattg ggtcagcttc aagtatttcc atcaagaagg
     9541 tcaactcggc ttcgtcagca tctgctaagg cttcatccat aatgctcaaa tcgtttttga
     9601 agtaccagct gctgatttca cttgcgtttc cggcattccc caatgaaccc ataaagcctg
     9661 catatacttc atttggcagc attatatccg caagcttcac agaatcacaa aaatatttat
     9721 attgacccaa gggatccagg cacaaaaaat ggagaagctc ttcctctccg tcatacccga
     9781 tccatacatg ataataatcg gatgtttcat caaccacata atatgtattt atcggataat
     9841 gggatatggg gttgttataa tctccgtcaa ggtacggcac agcaaagata tcgcacaaat
     9901 tcatcggccc ttcagccagc gtaacgttag taagagaggc aagatgcaaa ttctcggtct
     9961 gcttcattat gctggctgta gcgccagcct ccatataatt tgaaataaaa gcaatactca
    10021 agctggggta aatactctca tcctggaaat cacttgcaac cgcagaattc ccaacggact
    10081 gcagagtgaa ggtgaatccg ccttttgtcc cggctgcggg cgtggagggc gaaatatttc
    10141 tgaatgaaaa agctgtactg aaggaaaagt tctcacccaa ggtaaccttg tctgttgtaa
    10201 agacactttc gcctgtatca ccaccgcttt caaactgtat ggcattgttt tcaatcaggg
    10261 agccgccgtt caattgcaga ttctctgtat tgaaaaaatt gtcataactg atatcaacag
    10321 atggcgtact ttcagcagca taaacatctg gaattccatc aaaattcgta aatggaacag
    10381 gcagaccagt aaaaacgaaa ataaaagtaa gggcaatact aataattttt ctatggactt
    10441 tcatgacaat ttcccccttt taaacctcct aataataaaa gacacaagct aaaattttta
    10501 tttattggag ctccactaca aaaagaataa aatatgtgtt ataattattt tatagttata
    10561 atgaccgtag tcacatacta tagtcattat acaatatttg catgagacca gtcaatcaat
    10621 aggagaaata aatgatgaaa aaaaagaata tgagcaaaag aaaattgcag gcagctgaaa
    10681 caaaaaggaa aatctttgaa gccgcccggc agctgatcat agaacatgga attgaaaacg
    10741 taagtgtgga ttccatcgta aaagctgcag gagtatccaa aggcgctttt tatgtgcact
    10801 ttgaatccaa agatgcctta gcggctgctt ttgtcaatga ttatacaaac atagcagaca
    10861 tggattacaa atcctttttg gcatcgcttt ccgagcataa atccgctctt gatatccttg
    10921 ttctgttggc agaaaaaatt tccgatttta ttgatattaa tattggattt gaaaatatga
    10981 gggttctcta taaagcgcac ttaaccaaaa ctatcaatac agcatctgca atgagctata
    11041 acagggagct ctacaaattg ttcaccgatg cattggaaaa aggagtccat cagggagaga
    11101 taagagaaga catacctgtt gattctctgg caaaacactt aattctggcc atcagaggca
    11161 ttacctatga atggtgtatc aggtatcccg aactcagctt gaaggaacag gttctggatc
    11221 attttaaaat actgctgtac ggcttgaaaa gataaaaatt atttcgcctc ttcaaccctc
    11281 taaggattta tcaagctcac cgcaggccgg gcagataatg cgtgtatcgt ttatgatcat
    11341 atgtcacttc ctcacaaccc gtattttatg atctcaataa tttcttcctc gcacaaacaa
    11401 caggcaagtc cacaataaga aatttgttta ttcattgcat tacagccttt cggagatgac
    11461 ttttatattt ttgattacac ctttgtcatc ttttgcttaa acagctttgt accttttcta
    11521 aactaaaagg tgtcttcaat taataatcac agatgtggct cggcataaaa aacttcaata
    11581 ctggcatttt tttacaataa tattgtggtg tcatgccaaa ttgcttgtaa aacgctcttg
    11641 taaagccttc atgtgaatca aaaacaaagt caaacgccac atcaattatt ttagcattat
    11701 cgtctcgcaa cttgatagct gcccgtgaca atcgaagttc ctgaatgtat gcaaatggca
    11761 tcatacctgt cagttctttg aaaattgtgg ctcataagaa acaatatagc tggatgataa
    11821 aatattctca atcgtttttg ctgccagccg ccagtccctg ccggagaaca attctaaagt
    11881 gcagcatgac aataaatgag tgtctttttt ttaaaacatt ttcaaacata ttgacagaat
    11941 ttgatttaat gcatatactg ataacatatc aaacattttt gatatgaggt gataacttgg
    12001 gaacacttta tgtggaacac gcaaaagtat tcaaggcttt atgcgacgaa aaccggctac
    12061 gaatacttga tttattgcgt ggtgatgaaa aatgcgcttg tgtcttattg gaacagttag
    12121 acttgggaca atcagggctc tcctaccata tgaagatttt ggtagagtca ggtattgtgg
    12181 aaagtcggca agaaggtaaa tggacacatt acaaaattag cgaaaaggga agcacttatg
    12241 ctgtagctct tctaaaagaa ctgacagcac cgaacacggt tatagaagaa aataattgct
    12301 gcaaatgata gaagccatct gaacgatggc ttataacctg cataatacat caaaactttt
    12361 tgatatgatt gtccattttt agaaagaagg gttattttat gcaagtatta aaaacaggct
    12421 gggggttttt ccagaaccaa attctcggca tgaaatggct gaatgacttg atcggaagtg
    12481 tgttatcggt tttgggcctg gacacctcca atcgctggat cgggagcatt cagtttttca
    12541 tttatgacgt tataaaaatt gtagttctgt tatgcgtcct gatctttatt atcagctata
    12601 tacagagcta cttcccccca gaacgaagca agaagatatt aggacggttt catgggatcg
    12661 gagctaattc cgttgccgcc ttgcttggga ctgtaacgcc cttttgctcc tgctcgtcca
    12721 ttccactatt tattggcttt tcatccgcag gtttgccgct cggagtcact ttttcattct
    12781 tgatttcctc accaatggta gacctgggct ctcttgttct gctaatgagt atatttggta
    12841 caaaagttgc catattatat gtaatatttg gtttggcaat cgctataatc ggtggcactc
    12901 taatcgagaa agcgcacatg gaaaagcatg ttgagagctt tgtcttgaca gctggaagcg
    12961 ttgatatcga gtcacctaat ctggcgaaga aagaccggtt gatttatgca aatgaacaaa
    13021 cgctatctac ctttaaaaaa gtttttccat acatactaat cggtgtcgga ataggtgcaa
    13081 ttattcacaa ctggataccc gaggaatggg tggtatccct gcttggaagc aataacccct
    13141 tcggcgtaat cttagcgaca gtaatgggcg tgccgatgta tgctgatatc tttggcacaa
    13201 ttccgattgc tgaagcgctg ctcttcaaag gtgctcaact gggtacggtg ctgtctttta
    13261 tgatgggtat caccactctg tctttgccat ccataataat gctccgcaag gcggttaaac
    13321 cgaaactact ggcgttattt attaccgtct gcactgtggg tattatcctt gttggttatc
    13381 tattccactt tttacagcaa attacaatat aggaggaact tgatatggaa aaaacactgc
    13441 atcacgtcaa agactattca aaagggaaag agaaacaaat cgtcctgtgg ttgcggcggc
    13501 ggttgctgct gattatatga tttataaagg agaatacaga tatggcattt ttaaattttg
    13561 gaaagaaaaa aggaaaaact cccccctgct gttgcggagg tagctgcgat actgaaagta
    13621 tggcaaaggc agaaaaagca aaagccgagg gcgcaagtgt gaaggtactt ggcagcggct
    13681 gcgcaaaatg caatcaactc gaaggtgcaa caaaagcagc gcttgaacaa ctgggcatgg
    13741 atgcaacgat cgatcatgtg accgattttg cgcaaattgc agcgtacggt gtgatgacta
    13801 ctcctgctct tgttgtggac gggaaagtag tttcttatgg caaggttctc aagactgatg
    13861 aggtcgtaaa gattctgcag aaggtcagag tataaatata tagaaaaaag caaaaataca
    13921 ggaattagct tttctgaaaa atacctggta gtacctgtac aaaattgccc atccatgata
    13981 gtagctgccc ttacttgcag taaatctaat taatgcaaaa tccggctggt agcgaacaag
    14041 ttcgctgctc gccatgcatc ccgggtcagt ataccccaaa gggtagtatt ttttcattcc
    14101 gtcattccag aattctttcc tcttttcatc atcataggaa acttctgctt ttccggttag
    14161 catcaaaccc tcgaaggttt tcgcatcata gaaataaagg ctggcattag ggttttctgt
    14221 atctgtacta ctctctttga ggatgtattg gaacaaaacc agaattcctt tatcccgctt
    14281 ttacttttat ttggcggtcc atatactaca aatcctttat cattattact gtaagaaaaa
    14341 tgttgttcaa ggaa
//
LOCUS       DAOSDD010000102        14349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1669_length_14349_cov_4.893662, whole genome shotgun sequence.
ACCESSION   DAOSDD010000102 DAOSDD010000000
VERSION     DAOSDD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14349
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_14349_cov_4.893662"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="PK733_10310"
     CDS             complement(<1..1988)
                     /locus_tag="PK733_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10969.1"
                     /translation="MKKYKLVSFLLVAIFCMNIILPVILSSTTVLAIETTNTIYINSA
                     EDLISLAKKCSLDIWSQEKTVILNNNIDLSGVKFISIPTFGGIFDGQGYTISGLSLTD
                     YGSPQGLFCYVQEEALIKKLTVKGVVTPIGTKSIIGGVAGNNNGTIQNCIFNGVIRGD
                     KYIGGIAGINQAKGLISNCSVQGIAYGEHNVGGIAGENLGTILLSTNEANINTTVEES
                     SFNLENINSINVENLTSFSTADIVDISDIGGIAGFSSGIIQSCSNIGTVGYRNVGYNI
                     GGIVGRQSGYVNGCKNDGVIYGRKDVGGIAGQIEPYAFWELSENSLGKLRKELNTLQS
                     LINVTINNTDQYSSAISTQLAAVGKHISDTRTAGDVLVDQTISLLNDNIDNINNVSAR
                     ITQTLVAMEPIMGSFSAAIGDMKMAIGRFKTAMEQLEVTTRSAKFGMNRLNPALDKLD
                     AALGTTKKALDNISNSLTSLKAGMGDPKAVKDALSDMQKGITSLIDGMVQIADGARAL
                     YDAADTLQNSEVWQDNSTTIKNGANELIIGAGKMNDAMQAISNALILLEGDFDKEKLS
                     AALTSLESALANFASATEKLYSGFSKISSGFETGLEQIIHAYKGSEETQYTDSEETQR
                     EWYREIAEGVQEIASSMEDIQKSVRDLQDMLNYLQETN"
     gene            complement(1975..4551)
                     /locus_tag="PK733_10315"
     CDS             complement(1975..4551)
                     /locus_tag="PK733_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ10970.1"
                     /translation="MADSINIDKIGNNNQDNKFTIKFATFIVDKHNLFFFIFAALCIF
                     SVFSVNWVHVENELSVYLSDDAETKQGLKIMQEEFITYGTANVMIANISYEQAESIAE
                     QMKGLKGIFSVGFDKTKDHYNDASALFNVTFAYDEKDDNCVDALNVLLEEFKEYDIYV
                     GTGIGDIFIDTITREVGIVSIYIGIIVVAVLLFTSQSYAEVPVMLITFLSAALINMGT
                     NFLFGTISFISNSVAIALQLALSVDYAIILIHRFKEERETLPAREAIINALSKAILEI
                     SASSLTTICGLLAMTFMKFKVGLDMGIVLIKSIFLSLLSVFILMPGVLLAFSNLMDKS
                     RHRNFVPKIPFIGKFAYSTRKIIPPIFVLVLVASIFFSGKCPYVYGYDLLKTTKQNEL
                     QIVKAMIKDTFGSENPLALVVPAGKYESEAKLLAELENYDEVEYAMGLSNIEAMNGYM
                     LIDKLTPREFSELTDMDYEVVKLLYAAYAVKDKNYSEVVTGLDVYGVPLIDMFMFLYE
                     QVQEGYVKLDKDLQETLDDAYAQMNSAKQQLQGKNYSRILVNLNLPAEGDKTFRFLDT
                     IHEIAGKYYDDDVYLVGESSNNYDQSKAFSNDNTLVSVLSVVFVIIILLFTFKSVALP
                     IFLIMVIQGSIWTNFSFPYLTGSNMLFMNYLIASAIQMGANIDYAIVISNRYLELKLK
                     MPQREAIIETMNQSFPTLITSGSMMAVSGVLIGTMTSDPAISGMGESLGRGTIISLVL
                     VMFVLPQILLMGDKIIEKTSFTVNSPIRTQNTKGMIYVNGRIRGQVSGTIIGNLRGIV
                     RGDVNAVVESGEMKLLSEDPKFEDHKSEGPNSEDPNSKYHNSEDPKSEDPKSQEEKYE
                     KV"
     gene            5595..6392
                     /locus_tag="PK733_10320"
     CDS             5595..6392
                     /locus_tag="PK733_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ10971.1"
                     /translation="MGKGERNFHCGTLHHIIERGNNKNYIYKDTENKKKFFELLLQAM
                     EECDFKILYYVLMDNHYHLIIESGEIQASVGFHSLNNLYSKYYNKKYNRTGGNYEGKY
                     TSILITSARQYFQVLKYIANNPVRAKIVKNARDYRWSAHASFISGDNSIVEIERALSY
                     FPSPKAQVMEDYINLIEEGMKANYDYGLVPVKEAQLLSDSLDYILNSLNFSEDVFTKI
                     KNGDKRSDIKPERDCFIKEAYEAGFKIREIAEHISFTYEGVRKVVRG"
     gene            6437..6562
                     /locus_tag="PK733_10325"
     CDS             6437..6562
                     /locus_tag="PK733_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10972.1"
                     /translation="MEEGGGYGGRRRIWRKEVDMNEEGGYGGGRQIRRKEVGMKI"
     gene            6830..7525
                     /locus_tag="PK733_10330"
     CDS             6830..7525
                     /locus_tag="PK733_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ10973.1"
                     /translation="MKKLKIAAISIIAVLLILIGVFYVYTLDYYKADDFALNAFATEK
                     NNIQVQNNMIVFSPDKQNENEAALIFYPGGKVEAIAYIPLLEKLMQNGVTCVLVKMPF
                     NLAVFNINAADSIYEKLPEIKNWYIGGHSLGGAMASSYAEKNNNKLKGLILLGAYPVN
                     DSDITTITIYGSEDKILDKSKLENTGNMLEILGGNHAYFGNYGEQKGDGIAFITREEQ
                     QEETVEAIIRFIR"
     gene            7786..8529
                     /locus_tag="PK733_10335"
     CDS             7786..8529
                     /locus_tag="PK733_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOJ10974.1"
                     /translation="MEYFVIVIVIVIVIFITKLPSVKGNLGEISIRIWLKFLNKDKYK
                     VINNVMIPSGNGKPAQIDHIIVSVHGTFVIETKNYKGWIFGSENSPNWTQVIYESKNQ
                     FYNPILQNEGHVKALKNLLCDYPFIRYIPIVVFTSKAHFKKLDVTSHVIYSTELFRII
                     KNYTGQNISYDDAEKISKAILAANKDDEVTRKEHIARIQRNKFNRMKSNNGECPWCGK
                     ALVNRQGKYGRYTGCSNFPKCRFIMKGKR"
     gene            9067..9984
                     /locus_tag="PK733_10340"
     CDS             9067..9984
                     /locus_tag="PK733_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ10975.1"
                     /translation="MKFDFISRSVVKELLEENDYISNRNIEITIFNAIKLQVPLLIDG
                     PAGVGKTEIAKVLTKIFNASLIRVQCYEGIDFTKVLYDYNYSKQLLFVNLVKDNVKTI
                     IKGKDIHSSIELLNRETDFFGENFLIERPLLRAISPKDSSPKVLLIDEIDKSDVEFEA
                     FLLEMLSDFSVSIPEYGTISAEERPIVVLTSNATRELSEALKRRCIYLYIDYPVPEVE
                     AKILITKVNIEYDFANKIASAVKKIRSLPLKQKPSVAETINWSKALLLTMEEPDFSVH
                     NKVEIDSTLNVLLKNHNDIETVINSSYLV"
     gene            10004..11860
                     /locus_tag="PK733_10345"
     CDS             10004..11860
                     /locus_tag="PK733_10345"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOJ10976.1"
                     /translation="MENEYKWTSQDYLKLFGSIVNVPLEEDTFAITGFDKRGSSNHKL
                     INKNINASCKIIAYLSQIKQELGKSCFKKYSVEEIKEKLSVRSLEIIYKGLIQTKAIR
                     DYLPNITFDKAKYEDPFAKAHTEEWENIRRIIANYKNELSYLSDIYTREEYKSVIKNN
                     IKETLTIINNAEKVPCRNKRFIYSMERNYSGIIIEVKCSACEFSIKYNIEYNTKYNTE
                     QNTEHDIEHNTEHTTEYGAKHNTEYDASGCAIIDKAHLADRYLGFCSSINIEYKWFLS
                     KKDTVDALNIMVGEIRAHFADKVQKLLAEANRQNSNKVIGLLKRITDVSYIYNPKKDY
                     FTVFDVILTAENVQNNPEQIKRKIAGRIEQNIENAISEIQKHKQFYETLKNLSSLKIV
                     LECINAVAGYGRTTYIELIKGSRTQKMNEYGLHSSSYYGVLKQHTKKDIEEIINSLEE
                     HQLIKTYYGRYDRLYKYPKLKLTQAGVAEISKSLHEDRHEDRHEDRYEDRYEDKCEDK
                     CKNKYKGTQNEETNPLAFLKMFNMYTASEKVRLFKCVDMSKYTEIEFMEFLQFVLNRR
                     SSYKDVVNSMIQAFSTMPEKYIPIIEMNSQLTSGVAKETLEMIIDFVKAHNA"
     gene            11860..13356
                     /locus_tag="PK733_10350"
     CDS             11860..13356
                     /locus_tag="PK733_10350"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ10977.1"
                     /translation="MLDMDMDTNMDMDMLDLKDKFVMFIINFASFLRKCNFSIAPSAV
                     IDFFTICPQYDIFQYEEMLITIRSLFAKSQNEYTNFEILLQAYLYEIIDDRTSDYVKK
                     KAHEVNALLEELKRQKHSNISIKTRTTKEDKLLAQQADENKEAIVKILQDSGTVEILP
                     VIMLKEMEIVQLLASSSQEKVKSKLNQAIMFNIMYQNNALFNQLCIKGAKLFTKLNTK
                     VQKEITVRQRKITELEKLSTYTHRKDFIYGSRAVRTVYDIMDKPITDLGEDEYAQLVQ
                     YIKQNAAKFRTRISRNMKVSKRKIFDFKHTIEKSIETYGEPVRLYYKKPQIKKTRIVC
                     IADISGSVKQYIKLMLQLVYEISSVFKGGVRSYAFVGNLYNITEYMTRYPLEKGIMLA
                     SQQATREYSNYDKAFGIFVEDYIGFLDKHTIVLIIGDARNNRNDAGINYLKQIKNRSK
                     SIIWLNPEEKLKWNTGDSIIGKYKPIVNAVYQTNTFKEIIEFLDHFHL"
     gene            complement(13553..14287)
                     /locus_tag="PK733_10355"
     CDS             complement(13553..14287)
                     /locus_tag="PK733_10355"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOJ10978.1"
                     /translation="MILLDRYWKEIENLYYKVKSTQRENIIRAGNLIAEAVDQGACVH
                     IHDSGHIIDSELIYRGGGLILYKKFKYNLVVENPVRKRNRSDMDTSMEGLAAYALKAS
                     GARPGDVFILGSVSGRTFSVVDLAYEAKKLGMKLIVVTSMSYTTSVDSPHSSGKKLYE
                     MADVVIDNCAPAAEALMEAEGLEPRLCAASGLSAAFILWSVTTVVVEELMKRGKTPGV
                     LKSANFPGGNDYNKNYVEPRYEKEGL"
BASE COUNT         4976 a         2377 c         2714 g         4282 t
ORIGIN      
        1 atattagtct cctgcaaata attaagcata tcctgaagat ctcttacaga cttttgtata
       61 tcttccattg agcttgctat ctcctgtact ccttcagcta tttccctata ccattctcgt
      121 tgtgtttctt cactatccgt atactgtgtt tcttcactgc ctttataagc atgtatgatt
      181 tgttcaaggc cggtttcaaa accagaggat atctttgaaa aaccggaata taatttttca
      241 gtggctgacg caaagttcgc aagtgcactt tccagcgagg tgagtgcagc cgacagtttt
      301 tccttatcaa agtcaccctc caggagaatt aatgcattgc tgatggcttg cattgcatcg
      361 ttcattttgc ctgcaccaat tattagttca tttgcaccat ttttaatagt ggtagaatta
      421 tcctgccata cttcagaatt ctgcaaagta tcggctgcat catacaaagc ccttgcaccg
      481 tcagcaattt gaaccatccc gtcaataagg gaagttattc ccttctgcat atccgataat
      541 gcatccttga cagctttggg gtcacccatt ccggccttca aagaggttag tgagttcgaa
      601 atgttatcaa gagctttctt tgttgtgccc aaagcggcat caagcttgtc aagggcaggg
      661 ttaagcctgt tcataccaaa ttttgcactt cttgtcgtaa cctctagctg ttccattgct
      721 gtcttaaacc tgccaattgc catcttcata tcgcctatag cagcagaaaa tgagcccata
      781 attggttcca ttgcaaccag tgtttgtgtt atccgggcac ttacattatt tatgttgtcg
      841 atattatcat tcagcaatga aatagtctgg tctaccagca catcacctgc agttctagta
      901 tctgatatat gttttccgac tgctgcaagt tgtgtagaaa ttgcagatga atattggtct
      961 gtgttattga ttgtcacatt aataagtgac tgtaaagtat taagttcctt gcgaagcttg
     1021 ccaagggaat tctctgaaag ctcccaaaag gcatatggct caatttgacc tgcaatacca
     1081 cctacatcct tccgaccata gatgacccca tcatttttac atccgtttac atatccggat
     1141 tgtctcccta caatcccgcc gatattgtat cccacattcc gatatccaac tgtaccaatg
     1201 tttgaacagc tttggatgat tccgctggaa aaccctgcaa tacctccgat atctgaaata
     1261 tccacaatat ctgcagtgga aaaggaagtg aggttttcta cattaatgct attaatgttt
     1321 tccaggttaa agctgctttc ttctactgtt gtgtttatat tagcctcatt agtacttaat
     1381 agaatagtcc caaggttctc gccggctatg cctcccacat tatgctcgcc ataagcaatc
     1441 ccctgtacag aacaatttga aatcagtcct tttgcttgat ttatacctgc aataccgcct
     1501 atgtatttgt cgccccttat cactccgttg aaaatgcaat tctggattgt gccattgtta
     1561 ttgcctgcta caccaccaat gatagatttt gtaccaatag gagtaacaac tcctttaacg
     1621 gtaagtttct tgatcaacgc ctcctcctgg acataacaaa aaagcccctg gggagaaccg
     1681 taatctgtca gggaaaggcc gcttattgta tatccctggc catcaaaaat tcctccaaag
     1741 gttggtatgg aaataaactt tactcctgaa agatcaatat tgttgtttag gatgacagtc
     1801 ttctcctgag accaaatgtc aagtgaacat ttttttgcta aagaaataag gtcttctgca
     1861 gaattgatgt atattgtatt agtagtttca attgcaagca ctgtggtgct ggacagtatc
     1921 acaggcagta ttatattcat acaaaaaata gctacaagaa gaaatgaaac caatttatac
     1981 tttttcatat ttttcctcct gtgatttagg atcttctgat ttaggatctt ctgaattatg
     2041 atattttgaa ttaggatctt ctgaattagg accttctgat ttatgatctt cgaatttagg
     2101 gtcttcggat aataacttca tttcaccgga ttcaacgaca gcattgacat ccccgcgaac
     2161 gatgccccga aggtttccga tgattgtacc cgagacctga cccctaattc gcccgtttac
     2221 atatatcatt cccttggtat tttgtgtacg tatgggagaa tttactgtaa aggatgtctt
     2281 ttcaatgatt ttatctccca tcagcaaaat ttggggcaaa acgaacataa ccaaaacaag
     2341 tgaaataatt gtccctcgac caaggctttc acccattccg gaaattgcag ggtccgatgt
     2401 cattgttcca attaaaacgc ctgagaccgc catcattgaa ccggaagtaa ttaatgtagg
     2461 gaatgattgg ttcatcgtct ctatgattgc ttcccgctga ggcattttaa gcttcaattc
     2521 caaataacgg ttagaaatga caatggcata atcgatattt gcacccatct gaattgcact
     2581 agcaatcagg taattcataa aaagcatatt cgaacctgta agatatggaa aagagaagtt
     2641 agtccatatg ctaccctgaa taaccataat taggaatatg ggcagggcta ccgatttgaa
     2701 agtaaatagt aaaataataa taacgaacac gaccgataaa acactaacca gtgtattatc
     2761 attggaaaag gccttgcttt ggtcatagtt gttggaagat tcgcccacta aatagacatc
     2821 gtcatcatag tatttccccg caatttcatg aatagtatcc aaaaatctaa aggttttatc
     2881 cccttctgca ggaaggttta aatttactag aatacgactg taattcttac cctggagctg
     2941 ctgtttggca ctgttcatct gtgcataggc atcatccaat gtttcctgca aatccttatc
     3001 aagcttcaca tatccttcct gtacttgttc atataaaaac atgaacatat caatcaaagg
     3061 aacaccatac acatccaatc cggtaactac ctcactgtaa tttttatctt ttactgcata
     3121 ggccgcatac agaagcttaa ctacttcata gtccatatct gtcaactctg aaaactctct
     3181 tggagtcagt ttgtcaataa gcatatagcc gttcattgcc tctatattgg aaagccccat
     3241 ggcgtactct acttcatcat agttttctaa ttctgcaagc agcttggcct cagactcata
     3301 tttccctgcc ggtactacca gcgcaagggg attttctgaa ccgaaggtat ccttaatcat
     3361 agcttttaca atctgcaact cattctgctt tgttgttttt aagagatcat agccgtaaac
     3421 atagggacat ttccctgaaa aaaatattga tgcaactaaa accaaaacaa aaattggcgg
     3481 tattatcttt ctcgtagagt aggcaaactt cccaataaaa ggtatcttag gtacaaagtt
     3541 tctatgccgc gatttatcca ttaaattact gaatgctaac agaactcccg gcataagtat
     3601 gaatacggac agcaggctta aaaagattga ctttattaga acaataccca tgtctaatcc
     3661 aaccttgaac ttcataaagg tcattgccaa aagaccgcag atggtagtta aactactggc
     3721 tgaaatttcc agaattgctt tgcttaatgc gtttataatt gcctccctgg caggaagtgt
     3781 ttcccgttct tccttgaatc tatgaatcaa tattatggca tagtcaaccg ataatgcaag
     3841 ttgaagggct atggcaacag aattagatat gaaagatatt gtcccgaaaa gaaagtttgt
     3901 acccatattt attaatgcgg ctgataaaaa agtaatcagc ataaccggca cttccgcata
     3961 ggattgggaa gtaaaaagca aaacagctac aacaataatt ccaatatata tagatactat
     4021 tcctacttct ctggtaatag tatcaataaa tatatcacca ataccagtgc ctacatatat
     4081 atcatattcc ttgaactcct ctagcaaaac attaagagca tcaacacaat tatcatcctt
     4141 ctcatcataa gcaaaggtca cattaaaaag tgcggaagcg tcattataat gatcttttgt
     4201 tttgtcaaac ccaacggaaa aaataccttt aagacctttc atctgttccg caatagactc
     4261 agcctgttca taagaaatat ttgcaatcat aacatttgct gtaccatatg taatgaattc
     4321 ttcctgcata atttttagtc cttgttttgt ttccgcatca tcagaaaggt ataccgacaa
     4381 ctcattttcc acatgtaccc aattgacgga aaatactgaa aaaatacaca gggctgcaaa
     4441 tataaagaaa aataggttgt gcttatctac gataaaggta gcaaacttta tagtaaattt
     4501 attatcttga ttattgtttc ctattttatc tatgtttatg ctatctgcca tattttcatt
     4561 gcctcccagc gtattatgct actctattta ttataacgga gcccggttga aaatacaacg
     4621 ataagctcag tggtgttgta tttggtgccc ggcaccaaat acaacaccac caaaagtaga
     4681 atcaagaagt attcatttaa aatatggtta taattttgat aattatagta ataaatattt
     4741 tcgccagaaa aagcagagag ttataatatt tataaagaaa aaagatagta gttggatttc
     4801 gaaagatgaa ggtacaataa atgatgtttg taaaatatcc ggaattatcg atgagatgtt
     4861 gaaggtagta aaataatggt ttttatctat aaaattttaa cagaattatt aaattgggta
     4921 ataatgatga aacaattaat gatattataa aattgtaaat atataagaca tgcacaggtt
     4981 tataaggaag gtaaaattgg ctgtatcaat acttccggga aactggttgt gctttgtgtg
     5041 tacgatgaaa ttatcatgtt ctcagagggt ttggccatgg ttaaaaaaga tgacaagtgg
     5101 ggatttatca actctgtcgg ggaattaatg atctcttgca tatatagttc tgttcggcag
     5161 cttaatgttg tatttggtgc ctggcacaaa atacaacaag gcaccaaata caacaggcac
     5221 aaaataaaat ttattacaaa taaaaaaagt tattgaaata cttacaaaat taggttataa
     5281 tattttacat aaatagataa tatttactta agaggcgaat tattcatcta ttattaagaa
     5341 tatttgcatt ttaggtcttg aagtttgcct tattaatatg gaggtgaatg tttgtgatgg
     5401 tgtaataatg ataagtattg cagacctggg tgatgctgct gctaaagaga ccggagacgg
     5461 agttgagtct tgtataaatc agaatgaagg ggagtgattt aacattgatt tttcttatat
     5521 atctgacaac caagatataa ttgaatatgt tatttcagat actgagaatc acaattttat
     5581 ggcaggggga taatatggga aaaggagaaa gaaacttcca ctgcggaaca ctacatcaca
     5641 tcattgagcg tggaaataat aaaaattata tttataaaga tactgaaaat aagaaaaagt
     5701 tttttgaatt gcttttacaa gctatggaag aatgcgattt taagattctg tactatgttc
     5761 ttatggataa tcattatcac ttgattattg aatcaggaga aatacaagca agcgttggat
     5821 ttcatagctt gaataattta tatagcaagt attacaacaa aaaatacaat cgaactggtg
     5881 gtaactatga aggtaagtat acatcaattt taataactag cgcaagacag tatttccagg
     5941 tacttaaata catagctaat aatccggtca gagctaaaat tgtgaaaaat gcaagagatt
     6001 atagatggtc tgcacacgca tcctttattt caggagataa tagtatcgta gaaattgaaa
     6061 gggcactttc ttattttcca agtcctaaag ctcaggtcat ggaggactat attaatctga
     6121 ttgaggaggg tatgaaggct aattatgatt atgggcttgt tccagtaaaa gaggcacaat
     6181 tgttgtctga ttcactcgat tatatactga atagtttaaa cttttctgag gatgtgttta
     6241 caaaaatcaa aaatggtgat aaaaggagtg atatcaaacc cgaaagagat tgcttcatta
     6301 aggaggcgta cgaagccggg tttaaaataa gggaaatagc agagcatatt tcatttacat
     6361 atgaaggggt aaggaaggtg gtcagggggt aggtggatat gaacggggaa ggtgggtatg
     6421 gaggaaggag gcggatatgg aggaaggagg cggatatgga ggaaggaggc ggatatggag
     6481 gaaggaggtg gatatgaacg aggaaggtgg gtatggagga ggtaggcaga tacgaaggaa
     6541 ggaggtaggt atgaagatat agagaatatg caggagatag attgtttcca gtagcacata
     6601 ttaatgctat gagaaggtaa attattgagt ttctacgaaa ttggttatga tcctcacaaa
     6661 gatatgacaa tcgtcacgat aacaataaca ataacaataa caataacgat aacggtaacg
     6721 gcaacggcaa ctgtattggc gataataatg ataattagta tggtataatg gtgtatgaag
     6781 ctagacgggt atagctaatg gagcggcggg gaccagggag gaataggcgt tgaaaaaact
     6841 taaaatagct gccatcagta taattgcagt tctattaatt ttgataggtg tattctatgt
     6901 ctatacattg gattactaca aagcagatga tttcgcgtta aatgcctttg caacagaaaa
     6961 gaataatatt caagttcaaa ataatatgat agtatttagt cccgacaagc agaatgaaaa
     7021 cgaagcagca cttatatttt atcctggtgg taaggttgag gctattgctt atataccact
     7081 tctggaaaag cttatgcaaa atggagttac ttgtgttctt gtgaaaatgc catttaatct
     7141 tgcagtattt aatattaatg cagctgatag tatctatgaa aaactgccgg agattaaaaa
     7201 ctggtatata ggaggacatt ccctaggtgg agctatggca agcagttatg cggaaaagaa
     7261 caataacaaa ctgaagggtc ttattctatt gggtgcttat cctgtcaacg actcggatat
     7321 tacaactatt accatatacg ggagcgagga taaaattctt gacaaaagta aacttgaaaa
     7381 tactggaaac atgctagaga tattaggtgg caatcatgca tattttggca attatggcga
     7441 gcaaaaaggt gatggcattg cctttataac aagagaggaa caacaggaag agacagtgga
     7501 ggcaatcatt agatttatac ggtaggatta gtttgaaaga atgatgagat aaaactaaaa
     7561 tgagaacaag tccatttcag acgaaagtgg caccttgtgt tcacatatag aaagtacaac
     7621 aagcttgtgc gccagggtgt attttatgac cagcagcgtg gatgtgataa ttgaagtgta
     7681 gaattgaata acttagtacc ggcccaggta agttcgcgac cagataggaa aatgatgaaa
     7741 cacttgaagc gtaatccaat acatattttt gtgaggagaa acaaaatgga gtattttgta
     7801 attgtaattg taatcgtaat tgtaatattt attactaaat taccatctgt gaaaggtaat
     7861 cttggagaaa tatctattag gatatggcta aagtttttaa ataaagataa atataaagta
     7921 ataaataatg tgatgattcc atctggaaat ggtaaaccgg cacagattga tcatattatt
     7981 gtatcagtac atggtacttt tgttattgaa acaaagaatt ataaaggctg gatttttgga
     8041 agtgaaaaca gcccaaactg gacacaagtg atttacgaat caaaaaatca gttttacaat
     8101 ccaatcctgc agaatgaagg tcatgttaaa gcattgaaaa atttattatg cgattatccc
     8161 tttataagat atattccaat tgttgttttt acttcaaaag ctcattttaa gaaattagat
     8221 gtcacatcgc atgtcatata cagtactgag ctttttagaa taattaaaaa ttatactggg
     8281 caaaacatct catatgatga tgcagagaaa atatcaaaag ctattcttgc tgccaacaaa
     8341 gatgatgagg taacaagaaa agagcatata gcaagaatcc agcgaaataa atttaacaga
     8401 atgaaatcta ataatgggga atgcccatgg tgtggaaaag cccttgttaa taggcaaggc
     8461 aaatatggaa gatatacagg gtgtagtaat ttcccaaaat gcaggtttat tatgaaagga
     8521 aaaagataag ggagggatac aacaagctta aaagataatg gtttaatatg cgcaaatgtt
     8581 cgcttgtttg catataattt ttatgcattt attataatac ataacaagtt atatctgtaa
     8641 aagtgataac aggtataaat actgaaacag agaagttacg tgcagcaacc tacgccagtg
     8701 tttccacgtc gttgtatttt cagccgggca ccaaatgcaa caccaaatgt cgttgtattt
     8761 tcagccaggc accaaatgca acaggcatcg ttgtgttttc agccgggcac caaatgcaac
     8821 aggtaacaaa tgcaacaaaa atccaataca atttattaca tataatacaa aagatgttgc
     8881 aatatacata aaaatagttt ataataaatt accgtaatat acaataattc tatgagtggg
     8941 taaattattg cttctctaca agtacaaaaa tgaaatagaa tggattaatt aatgaaaggg
     9001 aaataaaaac gcattgcaaa cgcggctaaa atgaatccgc ccttgcagaa atggagctta
     9061 gttataatga aatttgattt tatatctcgt agtgtagtca aagaactatt ggaagagaat
     9121 gattatatta gtaacagaaa tattgaaatt actatattta atgcaataaa acttcaggtt
     9181 ccacttctta tagatggtcc tgcaggagtt ggcaagacag aaattgcaaa ggtacttacc
     9241 aaaattttta atgccagtct aattagagtt caatgttatg aaggcataga tttcacaaag
     9301 gtattgtatg attataacta ctcaaagcag ctgctttttg tcaatttagt aaaagacaat
     9361 gtcaaaacca ttattaaagg caaagatata catagttcta tagaattact caacagggaa
     9421 acagattttt ttggagaaaa ttttttaatc gaacggcctc ttctaagagc tatatctccg
     9481 aaagatagta gtccaaaagt gttattgata gatgaaattg ataaaagtga tgtggaattt
     9541 gaagcatttc tcctggagat gctatctgat ttttctgtat caattcctga atatggaacc
     9601 atatccgcag aagaaagacc tattgttgtt ttaacctcaa atgctaccag ggaactaagt
     9661 gaagctttaa aaagacggtg tatatatctt tatattgact atcccgttcc ggaggtggaa
     9721 gctaaaatat taatcacaaa agtaaatatt gaatatgatt ttgctaataa aatcgcgtca
     9781 gcagttaaaa aaatccggag tcttccatta aagcaaaagc cttctgttgc agaaactatt
     9841 aactggtcga aagcattgct tcttaccatg gaggaacctg atttttctgt acacaataaa
     9901 gtagaaatag atagtacatt gaatgttttg ctaaaaaatc ataatgatat tgaaacagta
     9961 ataaacagta gttatttagt ataggtttgg taaaggtgtt actatggaga atgaatataa
    10021 atggacaagc caggattatt taaagttatt tggtagcatt gtaaatgttc cactggaaga
    10081 ggatacattt gcaattactg gttttgacaa aagaggaagt tcaaatcata agctaattaa
    10141 taaaaacata aatgcaagct gtaagataat agcatactta tctcaaatta aacaagaatt
    10201 agggaaaagt tgttttaaaa aatatagtgt ggaggaaatt aaggaaaaat tatctgtacg
    10261 aagtttagaa attatatata aaggtttgat tcaaacaaag gctataaggg attacttacc
    10321 gaatattacc tttgataagg caaaatatga agatccattt gcaaaagccc atactgaaga
    10381 atgggaaaat atacggagaa ttattgctaa ttacaaaaac gagctttcat atctttccga
    10441 tatatacact agagaagaat ataaaagtgt tattaagaac aatataaagg aaacattaac
    10501 tataattaac aatgcagaaa aagtaccatg caggaacaaa cgctttatct acagtatgga
    10561 aagaaactat agtggaatta ttattgaagt aaagtgctct gcttgcgaat tttctataaa
    10621 atataatata gaatacaata caaaatataa tacggaacaa aatacagaac atgatataga
    10681 acataatact gaacatacta cagaatatgg tgcaaaacat aatacagaat atgacgcttc
    10741 tggttgtgca atcatagata aagcccatct agcagataga tacttgggtt tttgcagcag
    10801 tataaatatt gaatataaat ggttcttaag taaaaaagat actgttgatg ccttgaatat
    10861 catggttgga gaaataaggg cacattttgc tgataaggta caaaagttgt tggcagaagc
    10921 aaataggcaa aactcgaata aagttatcgg gttattgaaa agaataactg atgttagtta
    10981 tatttataat cctaaaaaag attactttac tgtgtttgat gtaatattaa ctgcagagaa
    11041 tgtacagaac aatcctgaac aaatcaaacg aaaaattgcc ggcaggatag agcagaatat
    11101 tgaaaatgct attagcgaaa ttcaaaagca taaacaattt tatgagactc taaaaaactt
    11161 gagcagttta aaaattgttc tcgaatgcat aaatgcggta gcaggctatg gacgcactac
    11221 ctatatagag ctgataaaag gctccaggac tcagaaaatg aatgaatatg gtttgcattc
    11281 gtcttcttat tatggtgtat taaaacagca cacaaaaaaa gatattgaag aaattataaa
    11341 ctctttagaa gagcaccaac taataaaaac atattatgga cggtatgacc gtttatataa
    11401 atatccaaaa ctcaagctga cgcaagcagg tgttgcggaa ataagcaaat cactgcatga
    11461 agatagacat gaagatagac atgaagatag atatgaagac agatatgaag ataaatgtga
    11521 agataaatgt aaaaataaat ataagggaac tcaaaatgag gaaacaaatc cattagcatt
    11581 tttaaaaatg ttcaatatgt atactgcttc ggaaaaagtc aggttattta aatgtgttga
    11641 tatgagcaag tatacggaaa tagaatttat ggaattccta caatttgtcc tgaaccggcg
    11701 atcttcatac aaggatgttg taaatagtat gatacaggcg tttagtacaa tgccggaaaa
    11761 atatattcca ataattgaaa tgaactctca attaacgagt ggtgttgcga aagaaacatt
    11821 agaaatgatt attgattttg taaaggcgca taatgcttaa tgctagatat ggatatggat
    11881 acaaatatgg atatggatat gttagattta aaagataaat ttgttatgtt tattataaat
    11941 tttgcttctt ttctaagaaa gtgtaatttt tcaattgctc caagcgcagt tattgatttt
    12001 tttacgattt gcccgcaata tgacattttt caatatgaag aaatgctgat aacaatcagg
    12061 tcgttatttg ctaaaagcca aaatgaatac actaatttcg aaatactttt acaggcgtat
    12121 ttatatgaaa taattgatga cagaacaagt gactatgtaa aaaagaaggc acatgaagtg
    12181 aatgctcttc tagaagaact gaaaagacaa aagcatagta atataagtat caaaacacgt
    12241 acaactaaag aagataagct gctggctcaa caagctgacg aaaataaaga agctatagta
    12301 aaaatcttgc aggattccgg tacggtagaa attttaccag taataatgct taaagagatg
    12361 gaaatagtcc aattgcttgc ttcttcctct caagaaaagg taaaaagcaa gttaaaccaa
    12421 gctataatgt ttaatataat gtaccaaaac aatgctcttt ttaatcaatt atgcattaaa
    12481 ggggcaaagc tttttactaa gttgaataca aaagtgcaga aagaaataac tgtacgacag
    12541 cgaaaaataa cagagttaga aaaactatct acatatacac atagaaaaga ttttatatat
    12601 ggaagcaggg cagtaaggac ggtttatgat attatggata aacctattac tgatctagga
    12661 gaagatgaat atgcacagct ggtgcagtat ataaaacaaa atgctgccaa gttcagaaca
    12721 cgtatatcta gaaatatgaa agtttcaaaa cgtaaaatat tcgactttaa acacactatc
    12781 gaaaaaagca tagaaactta tggagaaccg gtaagattat actataaaaa gccgcaaatc
    12841 aaaaaaacac gtattgtttg tattgcggat atttcaggtt ctgtaaaaca atatattaaa
    12901 ctcatgctgc agcttgttta tgaaatatcc tctgttttca aagggggtgt acgctcttat
    12961 gcgtttgttg gtaaccttta taatatcact gaatatatga caagataccc tctcgaaaaa
    13021 ggtattatgc ttgccagtca gcaagcaaca agggaatact cgaactacga caaagcattt
    13081 ggcatatttg tagaagacta tataggtttt ttagataagc ataccattgt attgattatt
    13141 ggagatgcaa gaaataatag aaatgatgca ggtataaatt atcttaaaca aataaaaaac
    13201 agatcgaaaa gtattatatg gttaaatcct gaagaaaaat tgaagtggaa cacaggtgac
    13261 agtatcatag gaaaatataa accaattgta aatgcagtat accaaaccaa tacattcaag
    13321 gaaataatag aatttttaga ccattttcat ctataatttt ctagtactac ggcacccgat
    13381 gctacagagc caggcatgtt tacaacgcta cagagccagg tatgttttac ggcgtcgcaa
    13441 ggggaaagtg ggcatttagg cgggtttggg gcagtgttgt gtttccgttc cgttgcattt
    13501 tcagccaggc accaaatgca acaccaaatg caacatttta aggcaagctt acttacaatc
    13561 cttctttttc atagcgtggt tcgacgtaat tcttgttata atcattccct ccagggaaat
    13621 tggcactctt gaggacaccg ggtgttttgc cccgtttcat caattcttcc actactactg
    13681 tggttacgct ccagagaata aacgccgcag ataagccgga tgcagcacat agacggggct
    13741 ccaggccttc tgcttccatt agtgcttctg ctgcaggtgc gcagttgtcg atgaccacat
    13801 cggccatctc atagagcttt ttgccgctgg aatggggact atccaccgat gttgtatagg
    13861 acatggaggt caccacgata agtttcatac caagcttctt cgcttcatat gcaagatcca
    13921 ccacgctgaa tgtgcggcca gatactgatc ccaggataaa gacatcgcct ggtctcgccc
    13981 cggaagcctt gagagcgtag gctgccagcc cttccattga agtgtccata tccgaacggt
    14041 tacgtttgcg cacgggattt tcaaccacca ggttatattt gaacttcttg tataatatca
    14101 atccgccgcc ccggtagatc agctcggagt caatgatatg cccggaatca tgaatgtgaa
    14161 cgcatgcccc ctgatcaacc gcctcagcaa tcaaatttcc tgcccggatg atattttcac
    14221 gctgggtaga tttcacttta taatatagat tttcaatttc cttccagtac cgatctagta
    14281 atatcatatt accctccttc ttctattaag tttaaattcc tttaaatatc tcctgttttc
    14341 ttagttttg
//
LOCUS       DAOSDD010000103        14293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1675_length_14293_cov_5.413190, whole genome shotgun sequence.
ACCESSION   DAOSDD010000103 DAOSDD010000000
VERSION     DAOSDD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_14293_cov_5.413190"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK733_10360"
     CDS             <1..258
                     /locus_tag="PK733_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10979.1"
                     /translation="IEVFGCFNKKRRENMYYYINLGVTYIKSINIDGKEYINNDYIPN
                     ACIKLGVPYIKTLKYNGVEYFNLDYKEPVEHNKDYRGKSLK"
     gene            complement(266..451)
                     /locus_tag="PK733_10365"
     CDS             complement(266..451)
                     /locus_tag="PK733_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10980.1"
                     /translation="MAIKYLHENPELVVINSNMIPPVLKFLGVQSMLLDRHVLTKGLP
                     QEDIEKIMDAVLALASN"
     regulatory      complement(729..819)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1049..1444)
                     /locus_tag="PK733_10370"
     CDS             complement(1049..1444)
                     /locus_tag="PK733_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ10981.1"
                     /translation="MIGNRIKQLRKEYNLTQHQFGNLFGLYDSTICLYESGKRTPEYN
                     IILKIADYFNVSVDWLLGRVDNKNLTMIENDKMPTELRNIGVEYLELAMEMSEQSIPP
                     EDVRKIMDAISALKNSISDDNNSKNSSEA"
     gene            1861..2529
                     /locus_tag="PK733_10375"
     CDS             1861..2529
                     /locus_tag="PK733_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOJ10982.1"
                     /translation="MELKEYFNILLKRAWLVIALPVIAALISAYVSFYMLTPVYEANT
                     TLYIINKTNGSEFQIAYNDLLISQQLVKDYKEFVKSRRVISQVIEQLGIEGISASAMA
                     AKVNVNAKNETRFIEIRVQDSNPALAADIANKLADVFKNEVVEYMKVENVNIVDTAQQ
                     PGNPIKPKPMMNIAVAFIAGLLFGAGLAFAIEYLDDTIKSAEDVEKYLGVTVLGAIPE
                     FSGK"
     gene            2578..3345
                     /locus_tag="PK733_10380"
     CDS             2578..3345
                     /locus_tag="PK733_10380"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ10983.1"
                     /translation="MALVEKKIYKLLYELNIPAEEAYKTLRSNIRFCGVVNKIKTITI
                     TSCVPGEGKTTTAVNLAMSMAKAGIKTLLVDVDLRRPMAEKLLKVNQDIGMTNYITGE
                     NSISEVVHSTGIENFYLIPCGPIPPNPAELLSSEMFSDFTKIIKDQYFEIIEGSFDMI
                     IFDTPPLGSVIDAALLAAQTDGTILVIKSKSINCKLAQQVKEQLDKANAYLLGVVLNH
                     VKRKDYQYGYGYGYKHYDYYYTKDKPKKTLFEKLFKR"
     gene            3399..4094
                     /locus_tag="PK733_10385"
     CDS             3399..4094
                     /locus_tag="PK733_10385"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10984.1"
                     /translation="MIDIHSHVIFDIDDGSKDLEESLKMMSDAEKAGVKTIIATPHFR
                     EHVLSPDKTFENLQILKEKSRDLGVDIRIGFEIILHPMLLQGEDIIEKHTIEGTRFIL
                     IEFPFTGFFNQSFTLLTKLQFRKLKPIIAHPERIEAFYKNRDIIVDLKGKGCLLQVNA
                     GSIVGYYGERARTFAKYIIKSRMADFVASDAHKPQYYLPMYKSAFSKVIKWTDNEYAE
                     KLFTKNGEAIFEV"
     gene            4257..5450
                     /locus_tag="PK733_10390"
     CDS             4257..5450
                     /locus_tag="PK733_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ10985.1"
                     /translation="MDQNKNKRIYLSPPHLNGYELAFVKEAFDTNWIAPLGPHTDAFE
                     NEIARYAGVKHALAISSGTAAIHLALKYSGVRQGNYVFCSSFTFAGSCNPIMYEKATP
                     VFIDSEPDSWNMSPAALEKAFQWADTRGKMPKAVIVVNLYGQSADWDRILPICQHYSV
                     PVIEDAAESLGATYKERQTGSFGYFGVFSFNGNKIITTSGGGMVVSNDEDAIKKLKFW
                     ATQAREPVRHYEHREIGYNYRLSNICAAIGLGQLKTIDERIDAKKRIWDKYKKAFKDI
                     PVKMMPVMPEGNPNWWLSAITVDNNCSKKPEDIIVALENENIESRPLWKPMHMQLVYK
                     DYPFFTHFSNERKSSNTCKNTEISKSIEVSEDLFKRGICLPSGSAITEEEQERVISVV
                     NEVLY"
     gene            5764..6003
                     /locus_tag="PK733_10395"
     CDS             5764..6003
                     /locus_tag="PK733_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ10986.1"
                     /translation="MNATGIVRKLDPLGRIVLPAELRKSLDLNVGAPLEIFVDGNKIL
                     LKKYEPVCVFCGEARDVSSYKGKLICNVCIDELRS"
     gene            6103..6603
                     /locus_tag="PK733_10400"
     CDS             6103..6603
                     /locus_tag="PK733_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ10987.1"
                     /translation="MIDKKQRKTKLRVYTHWLVVLFYMTMIFYFSSQDGTKSHNVSAG
                     LLGYIKLAVSLFSERFLEFFSYTGKNYEFFLRKTAHFTEYFVLSLLFYKTMIVSNIRA
                     GKSIVITLAFCFLYAVSDEIHQIFVPGRAFALRDVIIDTLGAAFGLSVLMIGKIIKER
                     TVKCKE"
     gene            6686..8563
                     /locus_tag="PK733_10405"
     CDS             6686..8563
                     /locus_tag="PK733_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOJ10988.1"
                     /translation="MRRMFKAFGVALVDVMLVLMSILLAFLLKYDFSITNIMNNYMAD
                     IAYILLVSLVIKIICFKIFKLYSSLWKYAGIYEAINAGMAVFVSNTLVIALCFMLPIS
                     VPVSVLIMNFLLDCILIIGIRFIYRIAYIVMKHKFNNNNSDESESKKLMIIGAGEAGS
                     VIVKELEHSFLNSRAAAIIDDDELKKGKKLHGVPIVGNRKDIVSIAERNNIDEIIIAI
                     PSAPRKDINEIFNECSKTKCKVRILPSISHLIDGKVSIKKVRDVQLEDLLGREPVKLD
                     IENIALYLKDKVVLVTGGGGSIGSELCRQIAAFLPQKLIILDNYENNAYDIQNELYHK
                     FPKLNIAVVIANIREKQRLESIFMKHSPEVVFHAAAHKHVPLMEDNPTEAIKNNVFGT
                     LNVAECADKYRVSRFILISTDKAVNPTNVMGATKRIAEMIIQAMDKHSKTEFAAVRFG
                     NVLGSNGSVIPLFKKQIEQGGPVTVTDPEITRFFMTIPEAAQLVIQAGAIAKGGEIFV
                     LDMGQPVKIHDLARNLIKLSGYEPDKDIKIVFSGLRPGEKLYEELLLKEEGLKATKHN
                     KIYVAKPIFTDLALLKRELGILYNLLTKDTENIVAYIPTIVPAYSRNNKVVSDRHYTN
                     I"
     gene            8590..11235
                     /locus_tag="PK733_10410"
     CDS             8590..11235
                     /locus_tag="PK733_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ10989.1"
                     /translation="MRKKLLAFVIIVAVLATQFCVTAVFADKADVKYAVDQLKGLSDE
                     NKGLLLQNIWDFCVAKIGDGMVISEDEILSAIINSLNDAGSGDLISDNQVAGDNKIGK
                     ESVRIIIRELLDYKDIIMDYYNQYKDIVTKSYVKSFLGLPPEATEGQVYVALLPYSVP
                     VITTDASTGKFDRNEDVSAAVARKLGADKGTFELLLGDMNSRIDELVDKIEAKIGVNG
                     ITRENVIYVLDIYTLYEYDPSAPAVSSSTPANNATGVGINSSITVKYSKNIYEDKLAA
                     ISLKSGSTEYAVQCSIEGGTLKITPKSPLAYNTTYTVTIPKGAVASEVMRQADAYTLT
                     FTTESQGGGGPVTPPSDDGTGGTTPTEPEDPKTDEPETEETKTLTEIEKIIESIDEKA
                     LSEATGTSLEKLAEETEAKVAKVSQLVDQVKDPEKVFEYAKELLDNIGTLPAKLEAAN
                     NYEAYDSISKELSKLAETVISIAGTLKVDVEAAEGKVEGEAKLGEVKAGELIDEGTSE
                     SLDLILAMAKQLNELLEKAGMEAEIEPLLYLNAVSGSDNVASAELKIPANLIIAAKEK
                     GIAGVVVTTDVAEFTVPADAIELKKDSVIVLNSEKKDNEALSDEVKKRVGDAPVYDFY
                     FTVDNERVAKFNKLITVSIPYTLKEGEDAEKVTVFYINEKGELENIIGVYDDETKRVY
                     FETEHFSMYTVKVNNVKFNDVASTFWAANYIEVMASKGVVKGIGGNLFAPDKNVTRAE
                     FVAMIVREFKLFDDKAVNNFSDVNEKDWFYPEVTSAAKLGIIQGRPGGIFAPNDKITR
                     EEMAKILSNVLTGIMGKKMPANKAAYLDSFKDKDKIADYAIDAVALGSRYKFFAGRPD
                     GTFAPKDNANRAEASKLIYMLFYME"
     gene            11484..12494
                     /locus_tag="PK733_10415"
     CDS             11484..12494
                     /locus_tag="PK733_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10990.1"
                     /translation="MKWKPLIIIVVVVSVLGVLSKVAIDHIFSFILMSQFSEINIDMN
                     SLLSGDNGGNIQSLPGSLDNKNNNIAEVNNINNANNKANNNANNKDIKNADNIKDPEN
                     ISTANGKNNNDGIIHNTKDSISNGDGSKIDVRASGNTNSDADCSNISSNTHGNEGSDI
                     SDTSGIDGKNQVVSNSDGDINNKIDSNSDDAANSNAGKNVTGNATGNIADGNSNNNGN
                     KSGDNIVNGIDDSHTVGGRNNSTDSQTSNGNSSIIVTEDKIKEAEKQVSTGDKLKALQ
                     ILLSKLEPSDIAILKNMLLGGKVTAEEMSKAKSIIKKRVTEEEKETLKEMYNKYNYLV
                     ED"
     gene            12580..14001
                     /gene="amrA"
                     /locus_tag="PK733_10420"
     CDS             12580..14001
                     /gene="amrA"
                     /locus_tag="PK733_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOJ10991.1"
                     /translation="MHGIINAYIFPHPPIIIPEVGRGRETDAANTIEAARRAAKDIRN
                     DNPDTIIITTPHGPFFEDYFHIPVTDFLQGDMGNFGAPKIKLKFKNNIDLINRIKTNA
                     LSEGIYAGGLDDKLMKEMRISQGMDHGAMVPLYFVSRELTDFKLVLISLANMPFKDLY
                     RFGMCISKAVAESGERIVFLASGDLSHSLTEDAPCGYNKKGEEFDKLLIKYISEGDVE
                     SILNFDRNFCEQAAECGLRSFIIMLGALDGYEIHPEVYSYEGPFGVGYSVAKIGIGKK
                     NSKRELLKEYERILKEKIDKIRAGEDHYISLARMALEAYVREDRLIKPYEIGYLPEEM
                     LNERAGAFVSIKKDGNLRGCIGTTGPTRGNLAEEIIHNAISAGTKDPRFYPVEEVELG
                     ELVYSVDILKEPERVKSIEELDVIRYGVIVRSGFKSGLLLPNLEGVNTPEEQVSIALK
                     KAGIKPYEKYTMERFEVIRHEVK"
     gene            14098..>14293
                     /locus_tag="PK733_10425"
     CDS             14098..>14293
                     /locus_tag="PK733_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ10992.1"
                     /translation="MGNRLMSRKAMYFEKIENGKVHCYLCPHNCIISPEKVGMCRVRQ
                     NIDGELYSLNYGKITSIALDP"
BASE COUNT         5174 a         2045 c         2871 g         4203 t
ORIGIN      
        1 atagaggttt tcggctgttt taacaaaaaa aggagagaaa acatgtacta ttacataaat
       61 ttaggtgtta catatataaa atcaataaat attgatggca aagaatacat aaataatgac
      121 tacataccta atgcttgtat aaaattgggg gttccctata tcaaaacatt aaaatataat
      181 ggggttgaat attttaatct ggattataag gaacccgtag aacataataa ggattatagg
      241 ggaaaatcct taaaatgaaa aaaacttaat tactagcaag agccagtact gcgtccatga
      301 ttttttcaat atcctcctgg ggcagacctt tagttaaaac atgtctgtca agcagcatgg
      361 attgtactcc taaaaatttc aaaacaggag gtatcatgtt gctgttaata actacaagct
      421 ccggattttc atgtaaatat tttattgcca ttagtactaa ccaccttttt ataataatca
      481 ataaattagt aactatttga gcataaattg gtatttttta tagtatgcac aatatgtaca
      541 atatagaacg aaaaacagaa aataaaaaca aacaaataca aaacaaatgg gcacctggat
      601 ttaattaacc agttgcccac aacgattact tgaattataa atcgaataat atttgacttg
      661 tagaaaacaa atttgcatat caatgcatat atatattgtt attattattc taatataatt
      721 aataatgctt cggcaacctg gcaatcatag aaagaaaaac ctttccttag acccatagct
      781 ttgcgtcact gcctttcgac agatatgcta ttatcgtgca acaatattca gtttttgcaa
      841 acaccactac ttaagtgttt atcctattta cacatgttat atcaatagta catcattcaa
      901 aaatacatca ttcaaaaata catcattcaa aaatacatca ttcatactta actatctaat
      961 tatatataat attccatatt tcaaaaaaaa gcaataatat ttttcaaatt tcctaaattt
     1021 tcttaaattt ttaaaaaatt catgttaatc atgcctcact actgtttttg ctgttattat
     1081 catcgctaat gctgtttttc aaagcactta tagcgtccat aatttttcgt acatcctcag
     1141 gtggaatgct ctgctcactc atttccattg caagttccaa atattcgaca cctatatttc
     1201 gcaattctgt gggcatttta tcattttcaa tcatagtcag atttttatta tcaactcttc
     1261 ccaggagcca gtctacgctg acattaaaat agtcagctat ttttaagata atattatatt
     1321 caggagttct ttttccactt tcatacaagc atatagtact gtcgtataaa ccaaaaagat
     1381 ttccaaactg atgttgagtc agattatatt cttttcttaa ctgtttaatt ctatttccta
     1441 tcattaccta ttatgctcct tattggttaa attacatgat aatattaaat tctattacaa
     1501 catattatat tttacaagag gaattcttag taaagaggag gcaacattga ttaaaatgta
     1561 aaatgtaatc aaattgtaat aaataaacac taaaaaatta tataaaaaag tcgaaaaaat
     1621 ataaggtgac aaatttcatt aataaatata taataaataa tatgataaat gagcattgtt
     1681 atatgcctat gccataaatt aaattgttta atatgcaaaa atcataaaat atagaataaa
     1741 catggtaaat cggggaaata ggatatattt gggttgacaa aagtatgtta aaaaagtaaa
     1801 atgtcaatca gtaattatct agcataaccg agtcaataat tatgaaaagg tgaattaata
     1861 atggaactta aagaatattt taatatttta ttaaagagag cgtggctggt tattgcattg
     1921 cctgttatag cagccttaat ttcagcctat gtgagtttct atatgttaac tccggtatat
     1981 gaggcaaaca caactttata tataataaac aaaactaatg gatcggagtt tcaaattgca
     2041 tataacgatc ttctcataag tcagcaattg gtaaaagatt ataaggaatt tgtaaaaagc
     2101 aggcgggtga tcagccaggt tatagaacaa cttggtatag aaggtatctc tgcatctgct
     2161 atggctgcga aggttaatgt taatgccaaa aacgaaacaa ggtttattga gataagagta
     2221 caggatagta atccagcatt agctgcagat attgccaata agctggcaga tgtttttaaa
     2281 aatgaagtag tggaatatat gaaggttgaa aacgtaaata tagttgacac tgcgcaacaa
     2341 ccgggcaatc ctattaaacc caagccgatg atgaacatag ctgtagcttt tattgcgggt
     2401 ctgctttttg gtgcaggact ggcctttgca attgaatatc ttgatgatac cattaagtca
     2461 gcagaggatg tagaaaaata tctgggtgtt acagtacttg gcgcaatacc ggagttctca
     2521 ggtaagtaac actgatattt aaaatatgaa gttacaaata aattggggga aagatggatg
     2581 gcattagttg agaaaaaaat atataaatta ctttatgaat taaatatacc ggcagaagaa
     2641 gcctataaaa ctttaagatc taatatacgc ttttgtggtg tagtaaataa aattaaaaca
     2701 ataactataa cgagttgtgt gcctggagaa ggtaaaacaa caactgcagt aaacctggca
     2761 atgtctatgg caaaagcagg tataaaaaca ttgcttgtag atgtagactt aagaaggcca
     2821 atggccgaaa aattactgaa agtaaatcaa gatattggaa tgacaaacta tataacaggg
     2881 gaaaatagta ttagtgaagt tgtacatagc actggtattg aaaacttcta ccttattcca
     2941 tgcggtccaa tacctccaaa ccctgcagaa cttctaagct cagagatgtt ttccgacttt
     3001 acaaaaatca ttaaagacca atactttgaa attattgaag gaagttttga tatgataatt
     3061 ttcgacactc caccgctggg aagcgttatt gatgcagcat tgctggcagc gcagaccgac
     3121 ggtaccatac tggtaatcaa atcgaagtct attaactgca agcttgcaca acaagtaaaa
     3181 gagcagttgg ataaagcaaa tgcatacctt cttggtgtgg ttcttaatca tgtaaagagg
     3241 aaagattatc aatatggata tgggtatgga tacaagcact atgattatta ctatacaaag
     3301 gataagccaa aaaagacatt atttgaaaaa ctgtttaaaa ggtgataact atataaatat
     3361 agtagtcact aaaataagga tagaagggtt aaattacagt gattgatata cacagccacg
     3421 taatctttga tatagatgat ggttcaaagg atcttgaaga gtccctgaaa atgatgtctg
     3481 atgctgaaaa ggcaggggta aaaacaatta ttgccacacc acatttcagg gaacatgtat
     3541 tatcccctga caaaaccttt gaaaatttac agatattaaa agagaaatca agggatttag
     3601 gcgtagatat cagaatagga tttgagataa ttctccatcc catgttacta cagggtgaag
     3661 atattattga aaagcataca atagaaggaa caagatttat acttatagaa ttcccgttta
     3721 caggtttttt taatcaaagt tttacacttt tgacaaaact tcaatttagg aaactaaaac
     3781 ctataatagc gcatcctgag aggatagagg ctttctataa aaatagggat ataatagtcg
     3841 atctaaaagg taaaggctgc cttctgcagg taaatgcggg aagcatagtg ggttattatg
     3901 gtgaacgtgc aaggacattt gccaaatata taatcaagag cagaatggct gattttgtag
     3961 cgtctgatgc tcacaaaccg caatattatt taccaatgta taaaagtgca tttagtaagg
     4021 taataaagtg gacagacaat gaatatgctg aaaaattgtt tacaaaaaat ggggaagcta
     4081 tttttgaagt ataggaatct ataggagccc ccaaaaatta ccattaggtg attagaaagg
     4141 ggattcacct tcccgcttta agggtatatg ataacataaa tgaacataaa attggttcaa
     4201 ttattggaac ttattaatat catagtatag gaacatttat ttaggggccg attattttgg
     4261 accaaaataa aaataagcga atatatttat cacctcccca tctaaatggg tatgagcttg
     4321 catttgttaa agaagcattt gatacgaatt ggatagcacc cctaggacct catacagatg
     4381 catttgaaaa tgaaattgca agatatgcag gtgttaagca tgctttagcc atttcatcag
     4441 gaaccgcagc aatacattta gccctcaagt attcaggcgt ccgtcagggc aattatgtat
     4501 tttgttcatc atttacattt gcaggaagct gtaatccgat tatgtatgaa aaagcaacac
     4561 ctgttttcat tgactcagaa ccggattcct ggaatatgtc acctgcagcg ctggaaaaag
     4621 cttttcaatg ggctgataca cgaggcaaaa tgcctaaagc agtgattgtt gttaaccttt
     4681 atggccagag tgcggactgg gacaggatat tacccatatg ccagcattac agtgtacctg
     4741 ttatagaaga tgctgctgag tctctgggag caacatataa agaaaggcag acaggaagct
     4801 ttggatactt tggtgtattt tcttttaatg gcaacaagat aataactaca tccggcggag
     4861 gaatggttgt atcaaatgat gaagatgcaa taaagaaact taaattctgg gcaacacagg
     4921 ccagggaacc cgtcagacat tatgaacaca gggaaatagg ttacaactac cgattatcaa
     4981 atatttgtgc tgctattgga ttgggtcagc ttaaaacaat tgatgaacga atagatgcaa
     5041 aaaagagaat ctgggataaa tataaaaaag catttaagga tatcccagta aagatgatgc
     5101 cggtaatgcc ggaaggaaac ccaaattggt ggttatcagc aattactgtt gataataatt
     5161 gcagcaaaaa accggaggac attatcgttg cgttggagaa tgagaatatt gaatcccggc
     5221 cactatggaa gcccatgcac atgcagcttg tttacaaaga ttatccgttt tttacacatt
     5281 tcagcaatga aagaaaaagc tcaaacacct gtaaaaatac tgaaataagt aaaagtatag
     5341 aagtaagtga agatttattt aagaggggga tttgcttgcc ttcggggtcg gcaattacgg
     5401 aagaagagca ggaaagggta atatccgttg ttaatgaagt gctctattag ggtaaatttt
     5461 gagaataaca tataaaaatt cctgatactt gctaaaattt gctctaaatt atggaaggct
     5521 tacccataat tggcgggcgc atattttatt ttttaacaat ataattataa ttgttttatt
     5581 cagattattt tgtgagtttg atttcgcccg tagctgaatt taaaggctcc aagtaccgta
     5641 acaatagctt ctggagtata tttcctgaat aattttaaat gtacccagaa ttgtccaaaa
     5701 gtattgtata gaagtagcaa taatggtata atgtaaacaa aaatataaag gtggaatgct
     5761 taaatgaatg ctacggggat tgttagaaaa ttagacccgc ttggaaggat tgttttacct
     5821 gcggagctta ggaagtcgtt agatttgaat gtgggtgctc cattagaaat ctttgttgat
     5881 ggcaataaga tattgcttaa gaagtatgaa cctgtatgtg tattttgcgg agaagcaagg
     5941 gatgtttcaa gctataaggg gaaactaatt tgtaatgtat gtatcgatga attacgaagt
     6001 taatatgcat tgtatatttc agtttttctc aaaagtatat tagtagacat ggaaggaaac
     6061 aaacggatag gttaatttgt actgttctgt ttaatttgaa taatgataga taaaaaacaa
     6121 aggaaaacaa aattacgagt ttatacccat tggctagttg ttttatttta tatgactatg
     6181 attttttatt tttcaagtca ggatggaaca aaatcccata acgtttctgc ggggttactt
     6241 gggtatatta agttggctgt ttctttgttc tctgaaaggt ttcttgagtt tttttcttat
     6301 acaggtaaaa actatgagtt ttttttgaga aaaacagccc attttacaga atactttgtt
     6361 ttatcactgc ttttttataa aacaatgata gtcagcaaca taagagctgg aaaaagtatt
     6421 gttataactt tggcattttg ttttctatat gcagtttctg atgagattca tcagatattt
     6481 gttcctggga gagcctttgc tttaagagat gttattattg atacattagg tgctgctttt
     6541 ggacttagtg tattgatgat aggtaaaatt attaaggaaa gaacagtaaa atgtaaagaa
     6601 taagcaaatg atgacaataa aagaggcttt tagatatatg taaattctgg gaataaaata
     6661 aataataggg ggtggacgcg atattatgcg tcggatgttt aaagcttttg gggtagcttt
     6721 agttgatgtt atgttagtac taatgtcaat tttacttgct tttttgttga aatatgactt
     6781 tagtattact aatataatga ataattatat ggcagatatt gcatacatat tactagtttc
     6841 attagttatt aaaattatat gttttaagat tttcaaactt tacagcagcc tttggaaata
     6901 tgcaggtata tatgaagcta ttaacgcagg catggctgtt tttgtatcta atacattagt
     6961 tattgcttta tgttttatgt tgcctatatc tgttccagtc tctgtattga taatgaactt
     7021 tttgctggat tgcatactaa taattggtat aaggtttatt tacagaattg cttatattgt
     7081 tatgaaacat aaattcaata acaataattc tgatgaatct gaatctaaaa agcttatgat
     7141 tatcggtgct ggagaagcag gatcggttat tgtcaaagag cttgaacaca gctttttaaa
     7201 tagcagagca gcagctatta ttgatgatga tgaacttaag aaagggaaaa aactccatgg
     7261 agttcctata gtaggcaata gaaaagatat tgtaagcata gcagagagaa acaatataga
     7321 tgaaataatt attgccatac catcagcacc aagaaaagac attaatgaaa tattcaacga
     7381 gtgttcaaaa accaaatgta aggtaagaat acttccatcc atttctcatc tgattgatgg
     7441 aaaagtttcc attaaaaagg taagggatgt acaactggag gatttgcttg gaagggagcc
     7501 ggtgaagctt gatattgaga atatagcttt atatcttaaa gataaggttg tattggtaac
     7561 cgggggcgga ggatccattg gttctgagct ttgcaggcaa atagcagctt ttttgccaca
     7621 aaaattaata atacttgaca attatgaaaa taatgcctat gatatacaaa acgaactata
     7681 ccataaattc cctaaactga atatagctgt tgtaatcgca aatataagag aaaaacagag
     7741 acttgaaagt attttcatga agcacagtcc ggaggtagta ttccatgcgg cggctcacaa
     7801 gcatgttccc cttatggagg acaaccctac agaggccatt aagaataatg tgttcggtac
     7861 tttaaatgtt gctgaatgtg ctgataaata cagggtgtcc agatttatac ttatatctac
     7921 cgataaagca gtaaatccta ctaatgttat gggagctaca aagagaattg ctgaaatgat
     7981 tatccaagct atggacaagc atagtaaaac tgaatttgca gctgtaaggt ttggcaatgt
     8041 tttagggagt aatggaagtg ttattccatt atttaaaaag cagattgaac agggaggccc
     8101 ggttacggta actgatcctg aaataacaag gttttttatg accatacctg aggcagctca
     8161 attagttatt caggcaggtg ctattgctaa aggaggtgaa atattcgtcc ttgatatggg
     8221 gcaacctgta aaaattcatg atttagcaag aaatcttatt aagctttccg gatatgaacc
     8281 cgataaagat attaagatag ttttctcagg gttaaggccg ggagaaaaat tgtatgaaga
     8341 gctcctcctt aaggaggagg gtctaaaggc aacaaagcat aataaaatat acgttgcgaa
     8401 accaattttt accgatctgg cactgctaaa aagagaatta ggcattttat ataacctgct
     8461 tacaaaggat acagaaaaca tagttgctta tatacctact attgttccag cgtatagcag
     8521 aaataataag gtagtgtcgg acaggcatta caccaatata taatttatta aattaggagg
     8581 ttttatgcaa tgaggaaaaa gttattagca tttgtaataa tagtagcagt actagccaca
     8641 cagttctgtg tgacagcagt atttgcggat aaagctgatg taaagtatgc tgttgatcag
     8701 ttaaaagggt taagtgatga aaacaagggg ttattgctcc agaatatttg ggatttctgt
     8761 gtagcaaaga ttggtgatgg gatggtaatt tctgaagatg aaattctatc agcaattata
     8821 aacagcttaa atgatgctgg cagtggagac ttgatttctg ataaccaagt ggcgggcgat
     8881 aataaaattg gtaaagaatc cgtcagaata ataataagag aactattaga ttataaagat
     8941 ataattatgg attattataa tcagtacaaa gatattgtta cgaaatcata tgtaaagagt
     9001 tttctaggtc ttcctcctga agcaacagaa ggacaggttt atgtggcttt actgccttat
     9061 tctgttcccg taataacaac agatgcttca acaggtaaat ttgacagaaa tgaggatgta
     9121 tcagcagcag tagccagaaa gctaggagca gataagggaa catttgaact actccttgga
     9181 gatatgaaca gcaggattga cgagcttgtt gataaaatag aagctaagat tggtgtaaat
     9241 ggaattacca gggaaaatgt gatttatgtt ctggatatat atacccttta tgaatatgat
     9301 ccttcagctc ctgctgttag ctcatctact cctgcaaata atgctacagg cgtaggtatt
     9361 aattcttcaa ttactgttaa atatagcaag aatatatatg aagacaaatt ggctgcaatt
     9421 agtctcaagt cgggttcaac tgaatatgca gtgcaatgtt ccatagaagg tggtactctt
     9481 aaaattactc ccaaatcacc tctggcatat aatactacat acactgtaac aataccaaag
     9541 ggagctgtag caagtgaggt aatgaggcaa gcagatgcat atacacttac attcaccaca
     9601 gaaagccaag gtggtggcgg tcccgttact cctccctcag atgatggtac aggcggaaca
     9661 actccaacag aacctgaaga tccaaaaact gatgaacctg aaaccgaaga aacaaaaaca
     9721 ttgactgaaa tagaaaaaat tatcgaatca atagatgaga aagctttatc tgaagctaca
     9781 ggcacttcac tcgaaaagct tgcagaggaa acagaagcta aggttgccaa agtatctcag
     9841 cttgttgacc aagtgaaaga tcctgaaaag gtatttgagt atgcaaaaga acttctagat
     9901 aatattggaa ctctgccagc taaacttgaa gctgcaaaca attatgaagc atatgattca
     9961 atttcaaagg aattaagcaa gcttgctgaa acagtaataa gcattgcagg caccttaaag
    10021 gttgatgttg aagcagctga ggggaaagtt gaaggcgaag ctaaacttgg tgaggtaaaa
    10081 gctggagagc tcattgatga aggtacttca gaatcacttg atttgatact tgctatggca
    10141 aaacaattaa atgaattact tgaaaaagca ggaatggaag ctgaaattga gccattactt
    10201 tatcttaatg cggttagcgg ttctgataat gtagcttctg ctgaattgaa aatacctgca
    10261 aatctaatta ttgccgcaaa agagaagggt attgccggag tagttgttac aactgatgtt
    10321 gcagaattta cagttccagc tgatgctatt gaactgaaaa aggacagtgt tatagtactt
    10381 aattcagaga aaaaagacaa tgaagctctg tccgatgaag taaaaaagcg tgtaggtgat
    10441 gctccggtat atgattttta ctttacagtg gacaatgaaa gagtggcgaa attcaataag
    10501 ttaattacgg tttccatacc ttacacactc aaggagggtg aagatgcaga aaaagtaact
    10561 gtattctata taaatgaaaa aggtgaactg gaaaacatca ttggtgtata cgatgatgaa
    10621 accaaacgtg tatactttga aacagaacat tttagcatgt atacagttaa agtcaacaat
    10681 gtaaaattca acgatgtagc aagtactttc tgggcagcaa attatataga agttatggca
    10741 tcaaagggag ttgtcaaagg tataggcggt aatttatttg ctcctgacaa aaatgttacc
    10801 agagctgaat ttgtagcaat gatagtaagg gaattcaagc tgtttgatga caaagctgtg
    10861 aataacttta gcgatgtcaa tgaaaaagac tggttctatc cagaggtaac atctgctgca
    10921 aaactgggta ttatacaggg aagacctgga ggaatatttg ctccaaatga taaaataaca
    10981 agagaagaaa tggctaaaat acttagtaat gttcttacag gaataatggg caaaaaaatg
    11041 cctgcaaaca aggctgctta tctggattca tttaaagaca aggataagat tgcggattat
    11101 gctatagatg cagttgcatt aggctcaaga tataagttct ttgcaggaag acctgatggt
    11161 acctttgcac ctaaggataa tgccaacaga gctgaagcat ccaaactgat ttatatgcta
    11221 ttctacatgg aataaacagg taacgaatat ataataaaca ggtaacgaat atataagtaa
    11281 aaaaatcact ctgcataata gaatgcaggg tgatttttta ttttcattaa tataaataaa
    11341 aattttgata tacttgtaca aaatatgtta aaatagttat atcacaaagt gtttgttaga
    11401 gtagttatct gcccactacc ctaaagggta attgcttgca agaagtaaaa ggaaacaatt
    11461 atatattgaa ggggtatgtt tagatgaaat ggaaaccatt aattatcatt gtagttgttg
    11521 tttcggtgct tggagtattg agtaaagttg caattgacca tattttcagc tttattttaa
    11581 tgtcacaatt ttctgaaata aatatagaca tgaatagtct tttgtccggc gataatggtg
    11641 gcaatattca aagcttgcca ggtagtctgg ataataagaa taacaacata gctgaagtta
    11701 ataatattaa taatgcaaat aataaagcaa ataataatgc aaataataaa gatataaaaa
    11761 atgccgataa tattaaagac cctgaaaata ttagtactgc taatggtaag aataataatg
    11821 atggtattat acataacaca aaagacagca taagtaatgg agatggaagt aagatagatg
    11881 tcagggccag cggaaataca aatagtgatg cggactgtag taatataagc agtaatactc
    11941 atggtaatga aggtagtgat ataagcgata cttcaggtat tgatggaaaa aatcaagtag
    12001 taagtaactc agatggagat attaataata aaatagatag taattcagat gatgctgcaa
    12061 atagtaatgc aggtaaaaat gtaactggaa atgcaactgg aaacattgcc gacgggaaca
    12121 gtaacaataa tggtaataag agtggtgaca atattgttaa tggaatagat gacagccata
    12181 ctgtaggtgg ccggaataat agcactgaca gccaaaccag caatggaaat agttctatta
    12241 ttgttactga agataaaatc aaagaggctg aaaaacaagt aagtacaggt gataagctga
    12301 aagctttgca aatacttctt tcgaaacttg agccttcaga catagcaatt cttaaaaata
    12361 tgcttttggg cggcaaggtt acagctgaag agatgagtaa ggccaagagt attattaaaa
    12421 aaagagttac tgaagaagaa aaagaaactc taaaggaaat gtataacaag tacaactacc
    12481 ttgtagaaga ttaaaggaaa tgtttaagaa aaaaacaact acgaaaaata taattatcaa
    12541 aagtaaatat aaatgcaaat gtaaaggcaa ggtgatttta tgcatggaat aataaatgca
    12601 tacatttttc cccatccacc tataatcata cctgaagtag gcagagggag ggaaactgat
    12661 gccgctaata caatagaagc tgctagaaga gcagcaaagg atataagaaa tgataatcca
    12721 gatacgatta ttataactac accacatggg ccgttttttg aagactattt ccatatacct
    12781 gttacagatt ttttgcaagg tgatatggga aatttcggag caccaaaaat aaagcttaag
    12841 tttaaaaata atattgatct tattaataga attaaaacaa atgccctatc agaaggaata
    12901 tatgccgggg gattagatga caaattaatg aaagagatgc gtatttcaca agggatggat
    12961 cacggagcca tggtaccttt atattttgtc agcagggaac tgacggattt caagctggtg
    13021 ctcatatcac tggctaatat gccatttaag gatctatata gatttggtat gtgtataagc
    13081 aaggcagtgg cagaatcagg cgaaaggatt gtgttccttg caagcggcga cctttctcat
    13141 agtttgactg aagatgcgcc ttgtggttat aataaaaagg gagaagaatt tgacaagctt
    13201 ttgattaaat atatctcaga aggtgatgtc gagagcatac tcaactttga cagaaatttt
    13261 tgtgagcagg ctgccgaatg cggtctaaga tcctttataa ttatgctggg ggctctggat
    13321 ggttatgaaa tacatcccga agtgtactca tatgaaggcc cttttggggt tggatattcc
    13381 gtagccaaaa tcggcatcgg taagaaaaat tcaaaaagag agttattgaa agaatatgag
    13441 agaattctta aggaaaagat agataaaata agagcaggtg aggatcatta tatatctttg
    13501 gccaggatgg cattagaagc atatgtaaga gaagatagac tcataaaacc gtatgaaatt
    13561 ggatatttac cagaggaaat gcttaacgaa agggcagggg cgtttgtatc cataaaaaaa
    13621 gatggtaatt tgcgtggatg cataggtact acaggcccca ccaggggaaa tctggctgaa
    13681 gaaatcatcc ataatgctat aagtgcaggg acaaaagacc caaggtttta ccctgtggaa
    13741 gaggttgagc ttggagagct ggtatattca gttgatatat tgaaagagcc tgaacgagtc
    13801 aaatcaatag aagagttgga tgtaataaga tatggggtaa ttgtacgatc aggatttaaa
    13861 tcaggacttc tgcttcctaa tttggaaggc gttaatacgc cggaagagca ggtttcaata
    13921 gcccttaaga aagcagggat aaagccctat gaaaaatata caatggagag atttgaagtt
    13981 atcaggcatg aagtgaaata ggaacagcaa aataagaatt atcgtgcagc aaaactacag
    14041 tcacatagat atgacccggt ggaaaataca aggttgtata agaaggagct attggaggtg
    14101 gggaacagat taatgagcag aaaagccatg tactttgaga aaattgaaaa tggaaaggtg
    14161 cattgttatc tctgcccgca taactgcata ataagcccgg agaaggttgg tatgtgcagg
    14221 gtcaggcaga atattgacgg tgagctatat tccttaaatt atgggaagat tacgtctatt
    14281 gctcttgacc cta
//
LOCUS       DAOSDD010000104        14183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1686_length_14183_cov_5.009343, whole genome shotgun sequence.
ACCESSION   DAOSDD010000104 DAOSDD010000000
VERSION     DAOSDD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_14183_cov_5.009343"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            597..1502
                     /locus_tag="PK733_10430"
     CDS             597..1502
                     /locus_tag="PK733_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ10993.1"
                     /translation="MLKDIKYDIKNNKNLIIMSLIGILWVILFRYLPMWGVLTAFKRY
                     NLYKGFWASEWIGFKYFHQFFTDMYFTRLLRNTILLSFYSILWGFPAPIILALLLNEL
                     KDGPFKRISQTISYLPHFISTVIIVGLMMQILASDGVVNKLIVLLGMEKINFFMEPKY
                     FRTLYISSGIWQSVGWSSIIYLAAISGIDPQLYDAADIDGANKLRKARHITLPGISNT
                     IVVLLILSIGSLLSVGFEKVYLLQNPAIYETSDIISTYVYRRGIIDGEFGYATAIGLF
                     NSLVSFILLVFSNTLSKKYFDKGLY"
     gene            1536..2414
                     /locus_tag="PK733_10435"
     CDS             1536..2414
                     /locus_tag="PK733_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ10994.1"
                     /translation="MKSSNKINNKPMNIIIGVFLLLLDAVMLYPIIYVFSVSISDTFR
                     VIAGDVVLFPKGFSLESYKIILNDTYFTRSYLNTVIYSVLGTFITLMLVTLTAYPLSI
                     RKFRSKKYITYIHVFTMFFGGGMIPTYLVIKSLGLIDTIWAMVLPNAIGVWYLLIFRS
                     FFSDISYSLHESAYIDGANDWIILYKIIIPLSKPVIATISLFTIVAIWNDFFSALLYL
                     NDRNKHPLQMTLRRILLASQVTRKENFQAEDLTKTVSLTMKSAAIFITILPIILIYPF
                     IQKHFVKGVMIGSIKG"
     gene            2567..4180
                     /locus_tag="PK733_10440"
     CDS             2567..4180
                     /locus_tag="PK733_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ10995.1"
                     /translation="MKKYKKSLSFLLGLVIIIGLLAGCSTVNKSKESDKSTGSTTSDI
                     KEPVVNQKISPEKITVSFFTTEDAVPMNENWLVFKAIEEKTNINMKLMTVSYANYEEK
                     MNSLIASGDLPDLMTLRGNGEAAKKYGPDGAFVNLSANMDKMPNFKKYMDKFDSSANS
                     YKLSKASDGNIYSAPRINDFVWITETYSVRADILKKHSLKTPDTFDEIYNMLVELKKL
                     YPDSTPVVNRWGTNTFLGSVARFMHTGIGMQFDHAKNEYRYYPYDEDFKEALLFVQKL
                     YKNGLLDPEFASTSMDVFTEKQTTGKAFFMYLYFVENDQNTMLGKEISKNPEFSIEPI
                     LPPMYKGSRGKGNVVSSVDLGFSKAISSKSKYVEPLIKFIDWLYSEDGIETVLFGLEG
                     ETFKRDSQGRPVLLDTVKTTTNPTGQNDIESYYGLYKQEIYSVQSEYSVDVIFRGPLY
                     SKAQKMMVENGCYGKVSPVISLTAQDKEEVGNINTQVNTLRDETIIKIITGEYSIDKV
                     DDFLKKAKELKIDRVIEIYNNQYKKEYGK"
     gene            4615..6954
                     /locus_tag="PK733_10445"
     CDS             4615..6954
                     /locus_tag="PK733_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ10996.1"
                     /translation="MLNKKSEMPKRIMTATIYKVIASLLLIILIVATNSFILRNLFMR
                     ELTGRILTQFSYSITASDEKLSNVRNILAAYMSDPDISFISSTVDDVPKGESLIKTLK
                     VINKFSKDSVYNSTIENIYLYYKERGWIINKNEAIFSKDAFLNKLMVYENDDFANHLE
                     NISTAAIFPKTAVARRIAGTNPKTYYGVTFMTPINSKCVIIASVNSDKFMESIYMPEK
                     DKGGHFLITDNNDKLITYDSNFDISDNDLKYLTSAFPNNKSEIQSKNLYILHNKYIVF
                     YKHSASTNFKYYVLAPATLVDKQVLIINLLLIGSLLFSGVIVILIFYILKKDIYIPVT
                     RINNYICNLFPDENKVENDMEYIYSLIEKLHNKNIVMMKNNEKQKSMLSELFIRRVIE
                     GSSVEFEMPFIDDIFAKIRNYYIVYLNLEIYDDKSYDFLWNQIVNEIQSEIEIYSFQI
                     NRNEMYCIVNLHGNNSGINVEDKIVNNFRNNGNFPHVDNQGLKMIIDEIISKIASDVN
                     INLVCGISRKYSGNGNIKKALDESKNTVLYRCESNTNKNVFVYDEAIQNFKEKHPDIQ
                     ISLAEEIELATFTSTGCVESVQFFFKTKYEKLNALPFIVKKETYRYLFNMAFVIIKHK
                     LNDDKKEEFQNSYIRYYDTVENSLNLKLIRRCVEDLYYDIAVQINSLEDEWKTCRIIE
                     YVNNNYYKNDFSLGKIAEEFNVTTSYLSTYFKKATGQNFTYYVNNLKVEKAKVLLQST
                     SMDIKHISDDLGFTHPKHFIRLFKQFEGTTPGSYRKLLC"
     gene            7028..9394
                     /locus_tag="PK733_10450"
     CDS             7028..9394
                     /locus_tag="PK733_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10997.1"
                     /translation="MEKSNPSMNPDTNPNINKAYLEYIYNFTRDEFFNSDGEAYFGRQ
                     ILFLGDSHGWGEGAPNYQGASSGFNIHAPWIHNRGFFNRVIEYLYKKMDTQPWRCIPL
                     TGMNCNLKKDDIPEDIYVGGVYYYDGIYFGSQDGINVASVFIDEVTFTTVPAFIKGRY
                     WIYQSAADDYKDSEKRALNVFCNAETRGDLYSAKNYSYNADIGLLGKNVFSLAPDIDR
                     PTEEFYRVTAYNAPVENFSVVEKSSDLCAFQRGSRYDYRLETEAVPANYRGGGHLSPR
                     YNGVQHEINEEQYKFCVNKCGGIDRDGVLFLVLKGDLNTLPSWLKTKKQRIYVPELGA
                     LEIVSTEKLFANEDIFVKLSFQKADNNDYDQSNKADYGQLNKAGFDQPDIDFKVLESS
                     IYPGMIIYKSYYGESCVLIDIKTPTRYLVLNFITQREYGAKAKIELLSNLVKLNCRGS
                     RICSRCDKGLGCYDYLDYYGNEIPSVYRLALPGNNKSSLVPALNAIVDYNGSENLNIS
                     MNQINESDLNISVHSSMRHAGDRYANSIIIDTSDDINAGHEEFYIIDFRQKKKGTVKI
                     SIAGPGKYNTKKHPATNSEVLMSRGMLCGNMDLFRNWSMGGHSTGAWLGQEESYWKDT
                     ENHIDDAIRYMTASVKSVVIQAPLVNEWLKQTPVDIFKTRLTNIIEMLQCRDVIILTT
                     LGLKDKEFGAFGEDISGNNENNNKNDSESSKISFEQYSLAVQEWAKSQPVSEHYNITF
                     IDCRKYLMDFVYKGILRPDDLYINNGHPSPAANEIIADMLITAINFKY"
     gene            9514..11607
                     /locus_tag="PK733_10455"
     CDS             9514..11607
                     /locus_tag="PK733_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ10998.1"
                     /translation="MLNGSDKQCCSKKNSYQYVLRFAVQPGNNEDERFAQLVEFCKTA
                     HIDEVMFFINCEELNQGHLTIEETKPWMKVIEKGKKLLDPLDIKTSINPWTTTLHTDR
                     GRKLKEGQNFRLMVDPYGNKASAVACPLCSNWQKYIAEMYSYYATIKPNVVWVEDDFR
                     LHNHDPLIWGGCFCEHHMEEYSKLAVKFGAKHEGEKLTREEFVEGILKQGEVHPYRKV
                     WLDISRKTMIDLAELLGEAVHKVSPDTKVGLMSSSPSVHCAEGRNWNAVFKCLSGDNP
                     PVSRPHLPSYNEVTSQNYLLGFSDVSRMTKAFLPENTEVYPELENFQDTRFAKSKAFT
                     RFQIETSTLIGSQGITLNIFDMMGNGVLLNEGYQYTLADTKEFVNKLTELGLDTLKQK
                     GVKIPICQESSYTLKIQKGSRMDELYPKEAFWAQLLSAYGIANTYTRDRKHSDSIIAV
                     SGQYFRNLNNEELVDLFENNYILMEGEAAYTLWDMGFGYLAGIYDATWYKVNSGVQAY
                     EETCNGEIYSGIHNSRISSQGSIGPYLAINYSENKSFNKITEVKSPDGKTVGPGMALY
                     NDKVFILPYSASGGGYGAYLNTTRQEITQACLEKLFAYARPTMVVDAPYVSVYDYDLE
                     DRRVLVIVNASGDDLDEVKIKLTDDCAALALMNIREISRCERDDTRLSAAIENGILSV
                     EGLKRLEVKAFVIEC"
     gene            11893..12657
                     /locus_tag="PK733_10460"
     CDS             11893..12657
                     /locus_tag="PK733_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ10999.1"
                     /translation="MFRVLLVDDEPWDLLGMEKGFQWKDAGFSIIGQTTNPVDALKII
                     LEEVPDVVFTDIRMPEISGIELIKRARENDIHSEFVIVSGYSEFQYAQEVIKYNGYYY
                     LLKPIDLDEAQELLKRLSAHLIQKQNLKGEIHVEDDKKTQKDIYTYIPNESFKELLDY
                     VNTNYCNEVSLNELSEVFHLNTTYICDLFKAVTGMTLIEYVTDIRMKKACELLKTTRL
                     SLAEVAEETGYSDYYYFNKLFKRKMGMPPGKYRRGE"
     gene            12662..>14183
                     /locus_tag="PK733_10465"
     CDS             12662..>14183
                     /locus_tag="PK733_10465"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11000.1"
                     /translation="MLSFTNLRLRYQLSFLVIVAILAMIAVQLFYYLSFQSLTKERAQ
                     VYFNSMTNQIETKIDAINEDIERIANLITYSQSVQKYVFEEDILKRFKMFEFVKDNLN
                     YTLSSNNNIQNIIIVSKNSGVVKSSSDDVVKVYNKINKEYDLDNYNISKPLYTRIYTD
                     EIGKKLYYAYITPIYSFLQQPYDEKVSAACVILLKVDLIQNLIDNVSMPSNSLFILHD
                     KYETVIASSKRENIGEKLDQKTSAFLSSSSVYSSNGNKITYNKKECLYQLTLIKNTEW
                     KIICMVPIAELMKDMKPMLKIGFIINITTTIILLIIGIFIIKSVTNPVSNIIMDMERI
                     GERGNEYRIRIPGDNEIGKISENINLMLERIDTINRSNLAIKENLYTAEIAKKQAELS
                     FLQSQINPHFLYNTLECMKNIGVVYNIPEIIKISTSMARIFRYSIKEHEIVSVREEIE
                     SIKDYFSIISIRFMGKYQMIIDLKGEILNHKMPKMILQPIVKNSVYHGLETRSREGM"
BASE COUNT         5161 a         1899 c         2625 g         4498 t
ORIGIN      
        1 atagagtaat atatggcagt gatatgataa atctggatcc aaggtatgat tttggaagag
       61 ttgtactgtc aacactttca tatgaagtaa agaaaaagat attagctgaa aattttctaa
      121 gcttactgca aaattctcaa atgggaaagg tagcctgttg ttttgctaat ttctcataac
      181 tacccagagt gtaatgatac caagtagctt cctatttctt ttgcttttaa attgtgccct
      241 acccctgatc gaactagcag ctttagcagc tagttttttc aatgcaaggt atactttaat
      301 tctaattcta ataatttggg tattgtataa aaattgggta ttcaaataac aaaaattatg
      361 ggaatgaaag aataaattgg gtattgtaca tagcagcaac atcagataaa ataaaattca
      421 taataaacaa tatagttact aaatgctttt aaataaaatc tctattattt taaataagat
      481 ctttattagt tgtttataaa tgttcatata gtataaacaa taaatgtgta gttaaagcaa
      541 aagagaaatt caaataattc catatagaac agtgaaagga ttggtgatta tattaagtgc
      601 ttaaagatat taagtatgat ataaaaaata ataaaaatct aattataatg tctctcatag
      661 gcatattatg ggtaattcta tttagatatt tacctatgtg gggcgtgtta acagctttta
      721 aaaggtataa cctatacaaa ggcttttggg caagtgaatg gataggtttt aagtattttc
      781 accagttctt tacggatatg tattttacaa gattattaag gaatacaatt cttctcagtt
      841 tttatagcat tctatggggg ttccctgccc ccataatact tgctcttctt cttaatgaat
      901 taaaagatgg accattcaaa agaatcagcc agactataag ttatttgcca cactttattt
      961 ctacagtaat aatagttgga ttaatgatgc aaatacttgc ttcagatgga gtagtaaata
     1021 agcttattgt attgctagga atggaaaaga ttaatttttt tatggaaccc aaatacttcc
     1081 gtactttata tatttcatct ggaatatggc agtctgtagg ttggagttct ataatatact
     1141 tagcggctat atctggtata gatccacagc tatatgatgc agcagatata gatggagcta
     1201 acaagctacg caaagccagg catattacgt tacccggaat ctccaatact atagttgttt
     1261 tgcttatatt aagcataggt agtcttcttt cagttggctt tgaaaaagta tatttattgc
     1321 aaaatcctgc catatatgag acatcagaca tcatttccac atatgtttat cgaagaggga
     1381 taattgatgg agagtttgga tatgccactg caataggtct ttttaattct cttgtttcgt
     1441 ttatactctt ggtattttca aatacattat ctaaaaagta ttttgataag ggtttatatt
     1501 agctattagc atgaggaaag gttgtgtatt cgtgtatgaa atcttcgaat aaaataaata
     1561 acaagcctat gaatattata ataggtgtat ttttattact ccttgatgca gtaatgcttt
     1621 atccaataat atatgtattt tcagtttcaa taagtgatac tttccgggtt attgcagggg
     1681 atgtggtttt gtttcccaaa ggattttctt tggaatcata taaaataatt ttgaatgata
     1741 catattttac acgatcatat ctgaatacag taatttattc tgtacttggc acatttataa
     1801 ccttgatgtt agtaacttta acggcttatc cgctatcaat aaggaaattc agaagtaaaa
     1861 aatacataac atatattcat gtatttacaa tgtttttcgg tggcggcatg atacctactt
     1921 atttggtaat taaatcatta ggcctcattg atactatatg ggcaatggta ttacctaatg
     1981 ctataggtgt ttggtacttg ctaatattca ggtcattttt tagtgatata tcctattctc
     2041 ttcatgaatc agcatatatt gatggagcaa atgattggat aatactgtat aagataataa
     2101 tacctttgtc aaaaccggta atagctacaa tatcactttt tactatagtt gctatatgga
     2161 atgatttttt cagtgcatta ttgtatttga acgataggaa caagcatccg ctccagatga
     2221 ccttacggag gatcctatta gccagccagg taacgagaaa agagaatttc caggctgagg
     2281 atttaactaa aactgtaagt ttaactatga aatcagcagc aatattcatt acaatcttac
     2341 caataatttt gatatatcct tttattcaaa agcattttgt gaaaggagtg atgatagggt
     2401 caattaaagg ctaaaatgat ttcccacaaa ctacataagt atgttggggg tgataaaaaa
     2461 ttaaccgatg ctataaaaaa ttatcatata agacatcgct taacaactta gcatattagt
     2521 accaatttat ttattaaaat aataattgta taggaggttt tgcaaaatga aaaaatacaa
     2581 aaaatcatta tcttttctac tggggttagt tataataatt ggtttattgg caggttgcag
     2641 tactgttaat aaaagcaaag aaagtgataa gagtacgggt agcacaacat cagatatcaa
     2701 agaacctgtt gtaaatcaga aaataagtcc ggaaaaaatt acagtttcat tttttactac
     2761 agaagatgca gtaccaatga atgaaaactg gttagttttt aaagctattg aagaaaaaac
     2821 caacatcaat atgaagctca tgactgtttc atatgctaac tatgaagaaa aaatgaattc
     2881 tcttattgca tctggtgatt tgccagactt aatgacactt agaggtaatg gtgaagcagc
     2941 taaaaaatat ggtcccgatg gtgcttttgt taatttaagt gctaacatgg ataaaatgcc
     3001 aaacttcaaa aagtatatgg ataagtttga tagctctgca aattcatata agcttagtaa
     3061 agcaagtgat ggaaatatct attctgctcc aagaatcaat gattttgtgt ggataaccga
     3121 aacttattct gtaagagctg atatactaaa aaaacatagt ttgaaaactc cggatacatt
     3181 tgacgaaata tataatatgt tggttgaatt aaagaagctc tacccggatt caactcctgt
     3241 tgttaacaga tggggtacta atactttttt agggtctgta gcaagattta tgcacacagg
     3301 tattggaatg cagtttgatc atgctaaaaa tgaatatagg tattacccat atgatgaaga
     3361 ttttaaagaa gctttgttat ttgtgcaaaa attatataaa aatggattgc ttgatcctga
     3421 atttgcttcc acatcaatgg atgtatttac tgaaaagcaa acaaccggta aagcattttt
     3481 catgtattta tactttgttg aaaatgatca aaacacaatg ttaggtaaag aaattagtaa
     3541 aaaccctgag ttttctattg agcctattct tcctccaatg tacaagggtt ccagaggaaa
     3601 aggcaatgtg gtatctagtg ttgatttagg tttttctaaa gcaatatcca gcaaatctaa
     3661 atatgttgaa ccactaatca aatttatcga ttggttatat agtgaagatg gaattgagac
     3721 tgttcttttt ggacttgaag gggaaacctt taagcgagat tctcaaggta ggcctgtatt
     3781 actggatact gtaaaaacaa ccacaaatcc tacaggacaa aatgatattg aatcatatta
     3841 tggtttatat aagcaagaga tttattcagt acaaagcgaa tattctgtag acgttatttt
     3901 tagaggtcca ttatacagca aagctcaaaa aatgatggtt gaaaatggat gttatggaaa
     3961 agtatcgcca gttataagct taacggccca agataaggag gaagtaggca acattaatac
     4021 acaggttaac actttaagag atgaaaccat aataaaaata ataacaggtg agtattcgat
     4081 tgataaagtt gatgacttct taaaaaaggc aaaagagctt aagattgata gagttatcga
     4141 aatatataat aatcaatata aaaaggaata tggaaaataa aattatatag cactgtggaa
     4201 agtaaaatta tatagcacgg tattgattgc atagaaagaa tacaatataa ttatattcga
     4261 caagaggaca atactcacaa agcagaacat aaattcttca ttgctgagga aggtcctctt
     4321 tatagttata tttgaactat agtaaattat ttatattcac ttagctaatt attgggcagc
     4381 agagcagtac cattgtgaaa ttcttactat gaatgggaaa gaggtatagt ttggaagaaa
     4441 ttggtaaatt tctggaaaac taatgcgcat gtgcatattt ttgcacaaga attttaaaaa
     4501 aataaattgc acgcgcagct aaaatgaatt taagcctata aaaacttttc cataaaaaaa
     4561 gttttgttcc tgaaaggacg aattcatttc gcacgctgca ggaatggagc ttgaatgctt
     4621 aataaaaaaa gtgagatgcc aaaaagaatt atgacggcaa ctatatataa agtgatagct
     4681 tcgttactat taataatttt gatagttgct acaaatagtt ttatattgcg aaatttattt
     4741 atgagagaat tgacaggacg gattttaact cagttcagtt attcaataac tgctagtgat
     4801 gaaaaactct ctaatgtaag aaatatttta gcagcatata tgtctgatcc tgatatttct
     4861 tttatatcaa gcacagttga tgatgtacct aaaggagagt ctttaataaa gactttgaaa
     4921 gttataaata aattttctaa agacagtgta tataacagta ctatagaaaa tatatattta
     4981 tattataaag agagaggatg gattataaat aaaaatgaag caatttttag taaggatgcc
     5041 ttcttaaata agcttatggt atatgaaaac gatgattttg ctaatcatct ggagaatatt
     5101 tctacagcag caattttccc taaaaccgct gttgcaagaa gaattgcagg cactaatccc
     5161 aagacctatt atggtgtaac tttcatgaca cctataaata gtaaatgtgt gattattgca
     5221 tcagttaatt ctgataaatt tatggaaagt atttatatgc ctgaaaagga taaaggtgga
     5281 catttcctta taactgataa caatgataaa ctgattacat atgatagtaa ttttgatatc
     5341 tccgacaatg accttaagta tcttacatct gcttttccaa ataataaatc tgaaattcag
     5401 agtaagaatt tgtacatatt gcataataaa tatatagtat tttacaagca ttctgcaagt
     5461 acaaatttca aatattatgt attggctcct gctactttgg ttgataagca ggttttgatt
     5521 attaatcttc tattaatagg atctttactc ttttctggag tgatagttat tttgattttt
     5581 tatattctta aaaaggatat atatataccg gtaacaagaa ttaacaatta catatgcaat
     5641 cttttccctg atgaaaacaa ggttgaaaat gatatggaat atatatatag cctgatcgaa
     5701 aaacttcaca ataaaaatat tgtaatgatg aaaaacaatg agaaacagaa aagcatgctt
     5761 agtgaattgt ttatacgaag agtaattgaa ggtagttccg ttgaattcga aatgcctttt
     5821 attgatgata ttttcgctaa aatacgtaat tactatatag tatatttgaa tttggaaata
     5881 tatgatgata agtcatatga ctttttgtgg aatcagatag tgaatgaaat tcagagcgaa
     5941 attgaaatat attcgtttca aataaataga aatgaaatgt actgtattgt taatttgcat
     6001 ggaaacaatt ccggaattaa tgtggaggac aaaatagtaa ataattttcg aaataatgga
     6061 aactttccac atgtggataa tcagggttta aagatgataa tagatgagat aattagtaaa
     6121 attgcctcag atgtaaatat caatttggtc tgtggtatca gtagaaaata ctcgggaaat
     6181 ggaaacataa aaaaagcatt agatgaaagt aaaaatactg ttttatatag atgtgaaagt
     6241 aatactaata aaaatgtatt tgtatatgat gaagcaatac aaaatttcaa agagaagcat
     6301 cctgatatcc aaataagttt ggctgaggaa atagaattgg ctacttttac cagcacaggt
     6361 tgtgtagaaa gtgtgcagtt tttctttaaa actaaatatg agaaattaaa tgctttacct
     6421 ttcattgtaa aaaaagagac atatagatat ctttttaata tggcttttgt tattataaag
     6481 cataaattaa atgatgataa gaaagaggag tttcaaaata gctatatcag atattatgat
     6541 actgtagaaa atagcttgaa tttaaagctc ataagaagat gtgtagagga tttatattat
     6601 gatattgcag ttcaaataaa tagtcttgaa gatgaatgga aaacatgcag aataattgaa
     6661 tatgtaaata ataattatta caaaaatgat tttagtcttg gtaaaattgc tgaagagttt
     6721 aatgtaacaa catcatattt atcaacatat ttcaagaagg caaccggaca aaatttcact
     6781 tattatgtta acaatttaaa agtagaaaag gcaaaagtat tgcttcaatc tacttctatg
     6841 gatattaaac atatttccga tgatcttgga tttactcatc caaagcactt tatcagattg
     6901 tttaagcagt ttgaaggtac tactccggga tcttacagaa aattattatg ctgattgatt
     6961 tttcatatta ttgcggatga aaacttttta ttactatatg aggaactggt aaattggagg
     7021 tagttttgtg gaaaaaagta atcctagcat gaatcctgat acaaatccta acataaataa
     7081 ggcgtacctt gaatatattt ataatttcac ccgggatgaa tttttcaatt cagatggaga
     7141 ggcgtatttt ggaaggcaga tattattcct tggagatagt catgggtggg gagagggtgc
     7201 gcctaattat caaggtgcat cttccggttt taatattcac gccccctgga tacataatcg
     7261 agggtttttt aatagagtaa tagagtattt gtataaaaag atggatactc agccttggag
     7321 gtgtattcca ttaactggta tgaattgtaa tctgaaaaaa gatgacattc cggaagacat
     7381 atatgtaggc ggagtatatt attacgatgg tatatatttc ggtagtcaag atggtataaa
     7441 tgttgcatcg gtttttattg atgaagtaac attcacaact gttcctgctt ttattaaagg
     7501 aaggtattgg atatatcaat ctgccgctga tgactataaa gattccgaaa agagggcact
     7561 aaatgttttt tgtaatgcag aaacgcgagg tgacttatat tccgcaaaaa attactctta
     7621 caatgcagat attgggcttt taggcaagaa tgtgttttcg cttgcacctg atattgacag
     7681 acccactgaa gaattttaca gagttacagc ttataacgca ccagttgaaa atttttcggt
     7741 tgtggaaaaa tcttccgacc tttgcgcgtt tcaacgaggc tcaagatatg attatcgcct
     7801 ggaaaccgaa gcggtacccg ccaactatag aggcggggga catctatcgc ctcgttataa
     7861 tggtgtacag catgaaatta atgaagaaca gtacaagttt tgcgttaaca aatgcggagg
     7921 gatagataga gatggagttt tatttttggt attaaaaggt gatcttaata cattgccaag
     7981 ctggctaaaa acaaagaagc aaagaatcta tgtaccggag cttggagcat tagaaattgt
     8041 aagtactgaa aaactttttg caaatgaaga catttttgtt aaactatctt ttcagaaagc
     8101 tgataataat gattatgatc aatcaaataa agctgactat gggcaattaa ataaggctgg
     8161 ttttgaccag ccggatatag attttaaggt tttagaatca tctatatatc ctggtatgat
     8221 aatatataaa tcatattatg gtgaatcctg cgttttaata gacataaaaa ctcctacaag
     8281 atatttagtt ttaaacttta tcactcaaag agaatatggt gcaaaagcca aaattgagct
     8341 attaagcaat ttagttaagc taaattgcag aggaagtcgt atttgttcaa gatgtgataa
     8401 aggtttaggc tgctatgatt acttggacta ttatggtaat gaaattcctt cagtatacag
     8461 gctggcctta cccggaaata ataaaagctc cttggtacct gccctaaatg ctatagttga
     8521 ctataatggc agtgaaaact taaatataag tatgaaccaa attaatgaat cagatttaaa
     8581 tatttcagta catagcagta tgcgacatgc cggtgataga tatgccaata gcataataat
     8641 agacacatcc gatgatataa atgcaggaca tgaagaattc tatattatag actttaggca
     8701 aaaaaagaag ggtacagtaa aaatatccat agctggacct ggaaaatata atactaaaaa
     8761 acatccggca acaaattctg aagtgttaat gtcaagggga atgctatgcg gcaacatgga
     8821 cttgttcaga aattggtcta tgggtggcca ttcaacgggt gcatggcttg gacaggagga
     8881 aagctactgg aaagataccg aaaatcatat agatgatgca attagatata tgacagcaag
     8941 tgttaaaagt gtcgtaatac aagcacctct agtaaatgag tggttaaaac aaacacctgt
     9001 tgatatcttc aagacaagac taaccaatat tattgaaatg cttcaatgca gagatgtgat
     9061 aatattaaca actcttggcc ttaaggataa agagtttgga gcatttggcg aagatatatc
     9121 aggaaataat gaaaataaca acaaaaatga tagtgaaagt agcaaaatat cttttgaaca
     9181 atattcactt gctgtgcaag agtgggcaaa atctcaacct gtgtctgagc attataatat
     9241 tacctttatt gactgcagaa aatatttaat ggattttgtt tataaaggta tcttaagacc
     9301 tgatgaccta tacattaaca atggacatcc ttctcctgct gcaaatgaaa ttattgctga
     9361 tatgcttatt actgcaatta attttaagta ttgattatat tatctcagta agttataaga
     9421 tattaaatgg atatataaaa gcatataaaa gcatataaaa gcatataaaa gtacataaaa
     9481 atatatataa ataagaaaca tggagggata tttatgttga acggaagtga caaacagtgt
     9541 tgtagtaaaa aaaacagcta tcaatatgtt ttaagatttg cagtacagcc tggtaacaat
     9601 gaggatgaaa gatttgcgca gttggtagaa ttctgtaaaa cagcccatat tgatgaggtt
     9661 atgtttttca ttaactgtga ggaactaaac cagggacatt tgacaatcga agaaaccaaa
     9721 ccatggatga aggttataga aaaaggcaag aaattgcttg atccattaga tattaaaact
     9781 tccattaatc cttggactac cacattacat actgatagag gcagaaaact aaaagagggg
     9841 cagaatttca gactgatggt agatccatat ggaaacaagg catccgctgt agcatgtccc
     9901 ttatgttcaa actggcagaa atatatagca gaaatgtatt catattatgc aactataaaa
     9961 ccaaatgtag tatgggttga agacgatttc agactgcata atcatgatcc gcttatatgg
    10021 ggaggctgtt tttgcgaaca tcatatggaa gagtattcaa agcttgcagt gaaatttgga
    10081 gcaaaacatg aaggtgagaa gctgactcgt gaagaatttg tagaaggaat tcttaaacaa
    10141 ggagaagttc atccatacag aaaagtctgg cttgatattt caagaaaaac catgatagat
    10201 ttagcagaac tattaggaga ggcagtacat aaagtatcac cagatacgaa agtcggcctt
    10261 atgtcatcaa gcccttcagt acattgtgca gaaggaagga attggaatgc agtttttaag
    10321 tgcctttcag gtgataaccc accggtgagc agaccacacc ttcccagcta taacgaagtt
    10381 acttcacaaa attatctttt aggcttttca gatgtttcaa ggatgacaaa agcatttcta
    10441 cctgagaaca ctgaagtata tcctgaattg gagaattttc aggacaccag gtttgcaaaa
    10501 tcaaaagcat ttaccagatt ccagatagaa acctcaacac ttattggcag tcaaggaata
    10561 accctaaata tatttgacat gatgggtaat ggagtgctgt tgaatgaagg ttatcaatat
    10621 actttagcag atactaaaga atttgttaat aaattgacag aacttgggct tgacacatta
    10681 aaacagaaag gggtcaaaat tccaatttgc caggaatctt cttatacctt gaaaattcaa
    10741 aagggtagtc gtatggatga actatatccc aaagaagcat tctgggccca gcttctatct
    10801 gcatatggaa ttgcaaatac ctacactcgt gatagaaaac attcggattc tattattgca
    10861 gtttccggac agtatttccg aaacctgaat aacgaagaac tggtggattt atttgagaac
    10921 aactatattc ttatggaagg tgaagcagca tatactttat gggatatggg ttttggttac
    10981 cttgccggga tttatgatgc aacatggtat aaagttaatt ctggagttca ggcctatgaa
    11041 gaaacctgta atggagagat atatagcgga atccataaca gcagaatatc cagccagggt
    11101 tcaatagggc catatcttgc aattaactat agtgaaaata aaagttttaa taaaattaca
    11161 gaagtcaaga gtcctgatgg gaaaacagtt ggccctggga tggctttgta taatgataag
    11221 gtgtttatct tgccttattc tgcttctggt ggagggtatg gagcttacct taatacaacc
    11281 aggcaggaaa ttactcaagc ttgtcttgaa aagctttttg cttatgccag gcccacaatg
    11341 gttgtggacg ctccatatgt aagcgtttat gattatgacc tggaagatag gagggtgctt
    11401 gtcattgtaa atgcttcagg agatgacctt gatgaagtaa aaataaagtt gacagatgat
    11461 tgcgctgctt tagccttaat gaatattcgt gaaataagca gatgtgaaag agatgatacg
    11521 agattaagtg ctgctattga aaatgggatt ttaagtgtag agggactaaa acggcttgag
    11581 gttaaggctt ttgtgataga atgctaatgg agatttaata aaaattctgt tgaattatat
    11641 gcggagctgc aattgaatga aggcggttcc gctgtatcta tagcaacaca atagtgcatc
    11701 ccatatagaa attaccatgt tcaaattgag acaccttctt gcaccgttct taaatgtata
    11761 ctataatgat actcaagatg gcagaaaata tattaaagct taaatattag gtgcatactt
    11821 ggatcaaaat tagtataaac caatatcgtt taatttcaag cagattttgg ttttcttata
    11881 tggggggatt ttatgtttcg tgttttgctt gtagatgatg agccttggga ccttcttggc
    11941 atggaaaaag gtttccaatg gaaagatgca ggtttttcaa taattggaca aacaacaaat
    12001 cctgtggatg cacttaaaat aattttggaa gaagttccag atgtggtttt cacagatatc
    12061 agaatgccag agatatccgg tatagagctc ataaaaagag cacgcgaaaa cgatattcat
    12121 tctgaatttg taatcgtaag tgggtatagt gagtttcaat atgcgcagga agtaataaaa
    12181 tacaatggat attattattt gcttaaacct atcgatttgg atgaagcgca ggaactgctt
    12241 aaaaggcttt ctgcacattt gattcaaaaa caaaatttaa agggagaaat tcatgttgaa
    12301 gacgataaga aaactcagaa agatatatat acatatatac caaatgagag tttcaaagaa
    12361 ttactagatt acgttaacac caactattgc aacgaagtgt ctctaaatga gctttcagag
    12421 gtatttcatc tgaatacgac ttatatatgt gatttattta aagcggttac tggaatgact
    12481 ttaattgagt atgttaccga tattagaatg aaaaaagcct gcgaactgct aaaaactacg
    12541 agactttcat tagcagaagt ggcggaagaa acaggttata gtgactatta ttactttaat
    12601 aaattattta aaaggaaaat gggtatgcca ccggggaaat ataggagagg tgaataaata
    12661 gttgcttagc tttactaact tgagattacg ttaccagttg agttttctgg taattgtagc
    12721 tattttggca atgattgcgg ttcagctatt ctattatctg agttttcaat cgctgacaaa
    12781 agaaagagca caggtttatt ttaatagtat gacaaatcag attgaaacta aaatagatgc
    12841 tattaatgaa gatatcgaga gaatagctaa tttgattaca tacagccaaa gcgtacaaaa
    12901 atatgtattc gaagaagata tactaaagag atttaaaatg tttgaatttg taaaggacaa
    12961 tttgaattat acactatctt ccaataataa tatccagaac attataatag tcagtaaaaa
    13021 ttcaggtgtt gtgaagtcaa gttcagatga tgttgtaaag gtttacaata aaattaataa
    13081 agaatacgac ctagataatt ataatatttc taaaccatta tatacaagga tttatactga
    13141 tgaaattggg aaaaaattat attatgcata tattacacca atttattctt tccttcaaca
    13201 accttatgat gaaaaagtat ctgctgcatg tgttatttta ctaaaagtag atttaattca
    13261 gaatttgatt gataatgttt ctatgccttc aaattcactt tttatattac atgataaata
    13321 tgaaacagtt atagcttcca gtaagagaga aaatataggt gaaaagcttg atcaaaaaac
    13381 atctgcattt ttgagctctt cgtcagttta ttcttctaat ggcaataaaa taacttataa
    13441 taagaaagaa tgtttgtatc agcttacttt gataaaaaac acagaatgga aaattatttg
    13501 catggtacct attgccgaac ttatgaaaga tatgaaacca atgttaaaga taggattcat
    13561 tattaatata acgacaacta taattttatt aatcattggg atttttataa taaaaagtgt
    13621 tacaaatcct gtatcaaata ttattatgga tatggaaaga ataggcgagc ggggtaatga
    13681 gtatagaata aggatacctg gagataatga aatcggaaaa atctcagaaa atattaacct
    13741 catgctagaa agaattgata ctataaatag aagtaatttg gcgattaagg aaaatttata
    13801 cacagctgaa atagctaaaa aacaggcaga actgtctttt cttcagagcc agatcaaccc
    13861 tcattttctt tataacactc ttgaatgtat gaaaaacatt ggagtggtat ataatattcc
    13921 tgaaataatt aaaatatcta cctctatggc taggattttc agatatagta taaaggaaca
    13981 tgaaattgta agtgttagag aagaaattga aagtataaaa gattatttct caattatatc
    14041 catacgattt atgggtaaat accaaatgat aattgactta aagggagaaa tattaaatca
    14101 taaaatgcct aaaatgattt tacagccaat tgtaaaaaat tcagtatacc acggccttga
    14161 aactagaagc agagaaggta tgg
//
LOCUS       DAOSDD010000105        14056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1703_length_14056_cov_5.070638, whole genome shotgun sequence.
ACCESSION   DAOSDD010000105 DAOSDD010000000
VERSION     DAOSDD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_14056_cov_5.070638"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK733_10470"
     CDS             complement(<1..548)
                     /locus_tag="PK733_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ11001.1"
                     /translation="MSIKIDSALNETGKEVQMAGTQVVRVRMALDLVEEGNDDAGSHG
                     KQERVSRQLPPGVSSRMLYKDIVGIAWPSFVELMLTQLASMVDMMMVGQLGPWAISSV
                     GLTNQPKFLMMAMFVAMNVGATALVARYKGAGDKQKANMVLRQALLLTFFFSLVSSII
                     GFIYSESLIRFMGAADAETLAGG"
     gene            complement(869..1774)
                     /locus_tag="PK733_10475"
     CDS             complement(869..1774)
                     /locus_tag="PK733_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010167807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11002.1"
                     /translation="MDEVLRKKIQDPIEDKILYTIVYTVLILFTLTVLYPLIYILSAS
                     FSSGSAVSTGRVVLWPVEPSLEGYKAVFRHKHIVSGYTNTIFYTFAGTLINVAMTMVA
                     AYPLSRRDMQFKKFYMFLFVFTMYFGGGLIPTYILMTQIKFINTIWVMVIPGALSVYN
                     MIVTRTFIMSNIPQELLEAAQIDGCSDAKYFFIIVLPLSKAIIAVITLFYAVGHWNSY
                     FNALIYLNEPKRYPLQLILRTILVANQVNLSDIDDPELIAAKQGLADLLKYSLIVVST
                     VPILALYPFVQKFFIKGVMIGSIKG"
     gene            complement(1789..2739)
                     /locus_tag="PK733_10480"
     CDS             complement(1789..2739)
                     /locus_tag="PK733_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11003.1"
                     /translation="MDVLKNKIKKQDTWEIFKKRWQLHVFMLLPVIYILIFAYYPMFG
                     VQIAFKDFMPAEGIWGSQWVGFKHFEIFFKSYQFGRVITNTLRISLYSLVVGFPLPIV
                     FALLLNTIRNMRFKKIVQTITYIPHFISVVVLVGILVQVLNPVTGLYGSIYRLFNGAG
                     YPKNIMASPAAFTHLYVWSGIWQQLGWSSIIYVAALSNVDQELYEAAEIDGASRWKRV
                     LHIDLPSILPTIAILLILRAGSIMSVGFEKVYLMQNNLNLMQSEVISTYVYKVGMAAG
                     GGNFSYASAIGLFNSIINCTLLVFVNAVSRKLSDENISLW"
     gene            complement(2830..4611)
                     /locus_tag="PK733_10485"
     CDS             complement(2830..4611)
                     /locus_tag="PK733_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11004.1"
                     /translation="MNKRYISIMLLVCLVIGVFTSCSGSGKNVNETGSNNTSKTEQTS
                     ATDDTSARMSDVPGMTAPGVLPIVTKPVKMTLGLAQNAFVMDYEDNYMTKRVLDETGI
                     SLDFYLFPTAEANQKFEMMVSANQELPEIIFLYFSDAARANYGKNGILKPLNSYFKKY
                     SHFYDLAAITKEERKKIEAFGTSPDGNIYAFPTYVDTIADIPMYTWFINMPWLDNVGL
                     EIPTTTDELYNVLKTFKERDPNKNGKPDEIPLISSNGWNGDIYYLLINSFIHYNPSYL
                     FNVENGVLSVPVITEEYREALRYLNKLVKEDLLSPLSFTLDNAGLKAMLSIPNEQDTI
                     VGMFGGSTTTLFDSANEKSFEYEGAPVVKGPKGVSWCPNRIPAYIYAASITKYCKNPE
                     AAFRLFDYFTEEKRSLSNRYGEPGVHWMYRPDDPEAFDKMFPYGNAAGYEPLHARIPG
                     VDTPWLTQNKTMWNIQFCSILPNNVYSAGSSASPLVKYYGEAKEKGMSPESHRSYIFN
                     KSCKDKVSNIPPETVTTLIYTEEENAAITEISNSINTYISESLALFCTGGLDIEKDWD
                     SYVKNLKSMGLDLYLKTAQTAYDRMFK"
     gene            complement(4883..7096)
                     /locus_tag="PK733_10490"
     CDS             complement(4883..7096)
                     /locus_tag="PK733_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11005.1"
                     /translation="MYKRLIGKKFINTPVYWRFIITYLIIVVAVIAVLTPIYYKAMYI
                     SKGVYLQESNYGLMRSADDFEKTVEAVWRIPNTMDQSLYYTKVKLFRDEKFTNDYYIY
                     LNLTRNFFAQQCYSVNLPGESFMLFTNSGAAVTRNRIFESIENCFSEQLVYDEFGVDK
                     MIETAINMDKFKAFIPVSGISNNYTGKYDRFLTILTRQTNDSVVIGTLFSESKLLELF
                     NIPNLPKNTFFYIKDTNNNMLISHNLEYDPNKIKGDMTEITYSGITYSILRHVMSSIG
                     YEVIIGIPDNYFTELLVPLRVLYNNYIISAIIIGIILSIIFALFNYIPLRRLVDIPLL
                     INRNMKKPCTNEYRYLRELMEHSDYEIQKLRSSIEDMDNTLRTNLFIRLLHGNVNADG
                     ERSLAMQLIPQLASDYRIVIIKADISEDGKNVDYLSFLVYDQLLHFLPSDFLFGQLDR
                     AKTAVLMPDTQQNSKLFSEVIRQINSNISIHNITLAAGVSEAFTGMDKINTAFYHAQF
                     SLFIKGGDLISYYKSVPESEPLSIDLIELQKLYELIQAGKSWEVEDMLDETAFILLNG
                     ETYSSEDVRKTFYMMRFVFESVINNMQCKSVVLPEYRENATVQELFASLGNIANKVAL
                     ELEERKRNVNSELKKEFLNYINENFSSKSIYATTIADALGVSESYVYKLAKECTGFSL
                     NDYIKKLRAEKAAQLLKTTDLSVANISDACGFDVLKTFYRTFRKYYGITPSQYRE"
     gene            complement(7359..8675)
                     /locus_tag="PK733_10495"
     CDS             complement(7359..8675)
                     /locus_tag="PK733_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11006.1"
                     /translation="MSNFKAGYARLDITPPFGISIAGYYNQRIADGILDNLYASAVAV
                     SDGSNTAVVISADLIGIPQKKMNVYRKSIAERNSIPLEAVFIACTHTHTGPVLQSRLF
                     PEDPNYNDYLQRRLSDVTTMAIEDLKPASVSIGRSTAPGISFVRRFRMKDGKIQTNPG
                     VGNQNILEPIGTPDETVQLVRLHRDNAKEILLVNFQVHPDVIGGNKFSADYPKFVRDT
                     LEKALDNVHCIYFNGAQGDTNHINVFTTLDKANRGYEHSKHMGRCIAGSVLQIYTKTT
                     LVSSGQISFDQHNIEVPLNRVDKSMIPEAEKIIALHEAGRDEDIPFKGMELTTVVAEA
                     YRIKRLENGPDTFTLPLTAISIGNVCIAGVPGEPFTDIGRSIKSGSPFEMTFVCCCAN
                     GYEGYYPMQSAYDEGGYEARSSKYKPGVAEAIIADSINLLMKLKKV"
     gene            complement(8981..9361)
                     /locus_tag="PK733_10500"
     CDS             complement(8981..9361)
                     /locus_tag="PK733_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOJ11007.1"
                     /translation="MFILIDTNIFVDHLRGRNEATELLKSCINKYDSVYYSVITRIEL
                     MTGMRQSEESIISGLLEAFKEVDVSKEIAYSAGLYMNKYIKSHGINVPDAIIAASARN
                     LDACLFTLSLKHFPMQDVKIERPY"
     gene            complement(9369..9629)
                     /locus_tag="PK733_10505"
     CDS             complement(9369..9629)
                     /locus_tag="PK733_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOJ11008.1"
                     /translation="MERTQVYLDRSHKQELQKMAKEKRTTMAELVREAVVEYLAKSNE
                     ESEKKIINTKGLWADRTDITDSDSYVNEMREKWNITPSDTEV"
     gene            9875..10081
                     /locus_tag="PK733_10510"
     CDS             9875..10081
                     /locus_tag="PK733_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11009.1"
                     /translation="MKIIIKDPNTLRRAGIESLAKNLGPVGAVYFLRQYDIGEDDYTK
                     ERKELLKIISIEDVKKDMENFKDS"
     gene            complement(10215..11462)
                     /locus_tag="PK733_10515"
     CDS             complement(10215..11462)
                     /locus_tag="PK733_10515"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOJ11010.1"
                     /translation="MGKNICISKTAVKTAAKTTAMSILSLFLVMSLTSCQSANPKSIS
                     SLAANISDEALKKAGISLIAREGKGALLEKDGQRIIVLKGTPYEMGYQHGVLLKDEVA
                     ALAKIVIEKISEIKPGELKKAWDMAEKYIPERYKEELKGLAEGAEIKLEDAQLVNMFP
                     ELFHCSGIVLFGKATKDGELLHARILDYATEQGIQNHSIVMLMQPDGYNTFFNAGMAG
                     LIGSVTGMNDKQISVGEIGGGGEGQWEGIPMTFLMRMALEETEKLKDSVNTFKKNPRT
                     CEYFYVISDAKIKDARVLYCTPKTFMTVKPGQNHPFLPSPPPKDALIASAKERYTEAL
                     KRVKENYGNIDKDILIEILKRPVSMKSNLHNAVFIPKDLKMWLAVADDPSKTDFQACY
                     QTYYEYDMPLILKYYSSMVEIMK"
     gene            complement(12119..>14056)
                     /locus_tag="PK733_10520"
     CDS             complement(12119..>14056)
                     /locus_tag="PK733_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11011.1"
                     /translation="KISGLTIGGPHMNVTPIDPGRYKFTASYYIAEGTNTIGRISLAA
                     IYRDKDGKEIGTAPDDTRYKLSATAGEWHTIERDLYIESEDIKYIQIIVTVENVRRDE
                     ALYIDDVGLCRVGEEKNEKPDPNRPLNKGSSFEEPERNLWTFWTHEGAVMEYSTDNPK
                     TDDYSLKISGLTVGGPHMNISQMEPGKYKFRASYYIAEGTNTVGRISLHAYYKDKDGN
                     IIEELPEGTRYRLSATAGKWHTIERELNIENEAVKILHIIVKVENIRRDEVLYIDDVG
                     LYKAGEEDYRVDDEGNKIFEFQPDQNKVTVPLGIVDDMDEKGNNIVITNESIEIILTY
                     PWISNVLHQISSDKADKYNIEVKADKLTADIAEKLLNNVEAKEEVELELIGNMWFIEL
                     DVLDENNQKINNLKPLPVTVSLKTPRNKATKLTYIYNFTVDGQIKHLGGKIKDDTIEA
                     EVNYPITFAVLTYEKRFTDVLQEFWAIDVIKEMAAKGIIKGTSLTEFSPNKEATRAEF
                     IAMISRALGIEESSTIAFSDVKEGSWYSSLISGAAKAGLVTGTEDEKFYPDRNISREE
                     IAAIILRSYELLTGSKVLKDVELAFKDSAEISPWARSVVGTVSEMKLMIGTDKGTFEP
                     KRSATRAECAKSIKNLLDIIQ"
BASE COUNT         4258 a         2816 c         2159 g         4823 t
ORIGIN      
        1 ccaccagcta atgtttctgc atctgctgct cccataaaac gtatcaatga ttcagaatat
       61 ataaaaccaa taatggatga taccaaagaa aagaaaaagg taagtaatag tgcctgacga
      121 agcaccatat tagccttttg tttatcacct gctcctttat accttgcaac taaggctgta
      181 gcacctacat tcatagctac aaacattgcc atcattaaaa atttcggttg attggtcaac
      241 ccaacagaag aaatagccca tggtcctagt tgtccaacca tcatcatatc taccatagat
      301 gccagctgcg tcagcatcaa ctcaacaaaa gaaggccatg cgattcccac tatatcttta
      361 taaagcatcc tggagcttac acccggaggt agctgcctgc ttacccgttc ctgctttcca
      421 tgactacctg catcatcatt tccctcttct actaaatcca aagccatccg tactctaact
      481 acctgagttc cagccatctg aacttctttt ccagtttcat ttaacgcgct atctattttt
      541 atgctcacct ttatttatac acacccttta gcagtataag tgtaagtgcc aaggcaaata
      601 taaacacata tatttgcgta ttttctgcaa tattttatca tggatatgag aaattgccta
      661 attcgtgtta tcctgtttac tggtaaataa catacatata tatttttttc tcatttcaca
      721 ttcctggcaa agacattttt cttagtcagc ttgatattat acatttctga tctggtaaaa
      781 tgcattcctg atttaatgat atgcaattcc agttatatgc ctgaatttat ggcttaataa
      841 tacatgtttg tttaataata cctaactgct agcctttaat tgatcctatc ataacacctt
      901 taataaaaaa cttttgtaca aacggataca aagcaagaat tggcactgta gacactacaa
      961 tgagagagta tttcaaaagg tctgccagcc cctgcttagc ggcgataagt tcagggtcat
     1021 caatgtcgct taagttgact tgatttgcaa ctaagattgt acgcaaaatt aattgcagag
     1081 gatatcgctt gggttcattc aaataaatta atgcattaaa ataggagttc cagtgaccaa
     1141 cagcataaaa tagtgttata accgcaatga tagcctttga caacggcaag actattatga
     1201 aaaaatactt tgcatctgaa cagccgtcga tttgagcagc ttctagtaat tcctgcggta
     1261 tgtttgacat aataaaggtt cgagttacta tcatattata gacacttaac gcaccaggga
     1321 ttaccattac ccagattgta ttgataaatt ttatctgtgt cattagaata tatgtaggta
     1381 ttaggcctcc accaaaatac atagtaaaga caaacagaaa catataaaac tttttaaatt
     1441 gcatatcccg tcttgataat gggtatgccg caaccatagt cattgctaca tttattaaag
     1501 ttccggcaaa tgtataaaaa atcgtattag tatatccgga aacaatgtgt ttatgcctga
     1561 atactgcctt gtacccctca agacttggtt ccaccggcca aagtacaaca cgccctgttg
     1621 acactgcact gcctgaagaa aaagaagctg atagtatata tattaacgga tacaaaacag
     1681 ttaaagtaaa aagtattaat actgtgtaga ctatcgtata aagtatttta tcttcaattg
     1741 gatcttgtat tttttttctc aaaacttcat ccatttcatt catctccctt accaaaggct
     1801 tatgttttca tcactcaatt tacgtgatac agcatttaca aaaactagaa gagtacagtt
     1861 tataattgaa ttgaacaatc ctatagctga tgcataggag aaattacccc caccggcagc
     1921 cataccaact ttatatacat aagtagatat aacctcggat tgcatcaggt ttaagttgtt
     1981 ctgcatcagg tagacctttt caaaaccaac actcattatg gaccctgcac gtaaaatcag
     2041 aagaattgcg atagtaggca aaatgctagg caggtctata tgtaaaactc tcttccaacg
     2101 ggatgctcca tcaatttcag cagcttcgta tagctcttga tcaacatttg aaagtgcggc
     2161 tacgtatata atggaactcc atcctaattg ctgccatatc cccgaccata catagagatg
     2221 ggtgaaagcc gcaggagatg ccattatgtt tttcgggtaa cctgcgccat taaataagcg
     2281 gtagatgctg ccataaagtc cagtaacagg gtttagaact tgcactagta taccaacaag
     2341 tactactacc gaaataaaat gcggtatgta tgtgattgtc tgtactattt tcttgaatct
     2401 catattccgt atagtgttta gtaatagtgc aaacactatt ggcagaggga aacctactac
     2461 aagtgaataa agcgagatac gaagagtatt ggttataact ctcccgaact gataagattt
     2521 gaaaaatatt tcaaaatgct taaagcctac ccactggctc ccccatatac cttccgcagg
     2581 cataaaatcc ttaaaggcaa tctgaactcc gaacattggg taataagcga atatcaatat
     2641 gtatatcact ggcagcagca tgaatacatg aagctgccag cgttttttga agatttccca
     2701 tgtatcttgt ttctttattt tattctttaa aacatccata agctacccct tcccagtccc
     2761 ctctgaacct taatttcaaa aggggcagtt ttactacccc ttttgaataa atgcatatat
     2821 actttctgat tatttgaaca tcctgtcata tgcagtctgg gcggttttga gatatagatc
     2881 aagacccatg ctcttgagat tttttacata gctgtcccaa tccttttcaa tatccaaacc
     2941 acctgtgcag aaaagcgcca gactttcact tatataagta ttaattgagt ttgaaatttc
     3001 agtgatagcc gcattttcct cttctgtata gataagtgtt gttacagttt caggaggaat
     3061 attgctgact ttatccttgc aacttttgtt aaatatatat gatctatgac tttcaggaga
     3121 cattcctttt tctttcgctt caccataata tttaaccagt ggtgatgcag agctgccagc
     3181 actgtataca ttatttggca gtatagagca aaattgtatg ttccacattg ttttattttg
     3241 tgttaaccac ggagtatcta caccggggat tctggcatgt agtggttcat agcctgctgc
     3301 attaccatat ggaaacattt tatcaaaggc ctcaggatca tcaggcctgt acatccagtg
     3361 tacacctggt tcaccataac gatttgaaag gctccttttt tcctcggtaa aatagtcaaa
     3421 gagacgaaag gctgcttcag gatttttgca gtatttggtt atagaagcag cataaatata
     3481 agctggtatt ctgttgggac accaactgac acccttagga cctttcacca caggtgctcc
     3541 ttcgtattca aaagattttt cattagctga atcaaaaaga gtagttgtac tgccgccaaa
     3601 catacctaca attgtatctt gttcattagg gatggaaagc attgccttga gacctgcgtt
     3661 atcaagagta aatgacagag gcgataacaa atcctcttta accagtttgt taagatagcg
     3721 cagagcttca cgatattcct ctgtgataac aggaacagag agaacaccat tttcgacatt
     3781 gaatagataa gagggattgt agtgaatgaa agagttaatt aaaagatagt aaatatcccc
     3841 attccagcca ttactactaa taagcggaat ttcatccggc tttccatttt tatttggatc
     3901 tctttccttg aaagtcttca aaacattata cagttcgtca gtggttgtag gtatctcaag
     3961 ccctacatta tcaagccatg gcatattgat aaaccatgta tacattggta tatctgcaat
     4021 agtatctaca taagtcggaa aggcatatat attgccatca ggtgatgtgc cgaaggcttc
     4081 tatcttttta cgttcctcct tggttatagc tgctaaatca tagaaatgtg agtatttctt
     4141 aaaataactg ttaagtggtt ttaaaatacc atttttacca taatttgcac gtgcagcatc
     4201 cgaaaaataa aggaaaatta tttcaggaag ctcttggttt gcagaaacca tcatttcaaa
     4261 cttctgatta gcctcagcag taggaaaaag gtagaaatcc aagcttatcc cagtttcatc
     4321 taaaactctt ttggtcatat aattatcctc ataatccata acaaatgcat tttgtgccag
     4381 gcctaaggtc atttttaccg gtttggttac aattggtaaa acaccaggag cggtcattcc
     4441 gggtacatct gacattctgg cacttgtgtc atctgtggct gaagtctgct cagttttact
     4501 tgtattattg cttccggttt cattaacatt ttttccactt cctgaacagc tcgtgaacac
     4561 tccaatcacc agacacacaa gcagcataat tgatatataa cgtttattca tgtacttacc
     4621 ccctcataaa tatgaaattg aataaaactt ttagtatagt cgggtaccct cacttactta
     4681 atatgatttt aaattaattc atgtctaatt taaagtatca ttctaagatt agagtgacaa
     4741 attcgtcaat tttacagtga caatttgaaa atcaggtgac aatttcgtca taggaacatg
     4801 ggtatagaaa catgaggacc gttctcacgt ttaatctcat atttcgacaa aaatgaatga
     4861 cccgatacag taatacttta tattattctc tgtactgact gggtgtaatt ccatagtatt
     4921 ttctgaatgt tctgtaaaat gttttcaata catcaaatcc acaagcatcc gatatgttcg
     4981 caaccgacag atccgtggtt ttgagcagtt gtgcagcctt ttcggcccga agtttcttga
     5041 tataatcatt aagactaaat cctgtacact cttttgccag tttatagaca tagctttccg
     5101 atacacccaa agcatctgca atagttgtag cgtatattga tttactagaa aaattctcat
     5161 ttatataatt taaaaattct tttttaagct ctgaatttac attgcgtttt ctctcctcca
     5221 attccaaagc tactttattt gcaatattcc ccagtgaagc aaacagctct tgtacagtag
     5281 cattttcacg atattcaggt aatactactg atttacattg catgttatta attacagatt
     5341 caaaaacgaa acgcatcata tagaaagttt tccgtacatc ctcgctggaa tatgtctcac
     5401 catttaataa tataaaggct gtttcatcca gcatatcctc aacttcccat gacttgcctg
     5461 cttgaataag ttcataaagt ttctgtaatt caattaaatc tattgacagt ggttcgctct
     5521 ccggtacgga tttgtagtag ctgattaagt caccaccctt gataaataag ctaaactgag
     5581 catgataaaa agcagtattt atcttatcca tacctgtaaa tgcttcgctt acacctgcag
     5641 caagcgttat attatgaata ctgatattgc tgtttatctg cctgattacc tcagagaaaa
     5701 gcttggaatt ttgttgtgta tcaggcataa gtactgctgt ctttgcccga tccaactgcc
     5761 caaataaaaa atcagaaggg aggaagtgca gcaactgatc ataaacaaga aagcttaaat
     5821 aatcaacatt cttcccatct tcagaaatat cagcttttat aataacaatg cggtaatccg
     5881 atgcaagctg tggaataagc tgcatggcta aacttcgttc tccatcagca tttacatttc
     5941 catgcagcag tctgataaaa agatttgtgc gcagagtatt atccatatcc tcaatactac
     6001 tacgcagttt ctgtatttca taatctgaat gttccataag ttctctcaaa taacggtatt
     6061 catttgtaca tggtttcttc atgttgcgat taatcagcaa tggtatgtct acgagtcttc
     6121 taagcggtat atagttgaat aaagcaaaaa taatacttag tattatacct attataatag
     6181 ctgatataat gtaattatta tataatactc ttaaaggaac aagcagctca gtaaaataat
     6241 tatccggaat accgattatt acctcataac ctatagagct cattacatgc cgcaatatac
     6301 tatacgtaat accactatat gtaatttctg tcatatcccc cttgatttta tttggatcat
     6361 attctaaatt atgagatatc agcatattgt tgttcgtatc ttttatataa aaaaacgtat
     6421 ttttcggaag attaggaata ttgaacaact caagcaactt gctctcacta aaaagcgtcc
     6481 caataacaac actatcgttt gtctgacgag ttaaaattgt tagaaatcta tcatacttgc
     6541 cagtataatt atttgaaata cccgatacag gaataaatgc tttgaattta tccatattaa
     6601 ttgctgtctc aatcatcttg tcaacaccaa actcatcata tacaagttgt tctgaaaaac
     6661 aattttcaat ggattcgaaa atgcggtttc ttgttactgc tgccccactg tttgtaaaga
     6721 gcatgaagct ctctcccgga agattaacag aatagcactg ctgtgcaaag aaattccgcg
     6781 taagattcaa atatatataa taatcatttg taaatttttc atcacgaaaa agctttactt
     6841 tagtataata cagggattga tccatagtgt ttggaatgcg ccaaactgcc tctactgttt
     6901 tttcaaaatc atcagcactt cgcattaggc catagtttga ttcctgcagg taaactcctt
     6961 tcgatatata catagcttta taatatatgg gagttagcac agctataact gcaacaacaa
     7021 taataagata tgtaattata aatcgccaat acactggagt atttataaat ttttttccta
     7081 ttaatctttt atacattagt attaaccccc tgtaaatgct tcgcagttca tttttgcaat
     7141 gagactttct ataactatgc atcattaaag aaacacaaga accgtctcca tgtttccagg
     7201 aacaccgccc ccatgtttct atgctttttc ttccctgact atatagaaca ggtataaaat
     7261 atttgaaacc tgcttctgtt accttgatgt aacttacgat ttgcaaattc ttcattagtt
     7321 ctttcaaatc gtaaattata tagcaacatt tattcagttt ataccttttt aagtttcata
     7381 agtaaattta tagaatctgc aataattgct tcggcaacac ctggtttata tttagaacta
     7441 cgagcctcat accctccttc atcataagcc gattgcatag gatagtagcc ttcatagcca
     7501 tttgcacagc agcaaacaaa ggtcatttca aatggagaac cactctttat gctgcgtccg
     7561 atatctgtaa acggctcacc aggtacaccg gcaatacaaa cattaccaat gctgatagca
     7621 gtaagaggaa gagtaaacgt atcaggccca ttttcaagcc gttttatgcg atatgcctca
     7681 gcaactactg ttgtaagttc cattccttta aatggaatat cttcatctcg tcctgcttca
     7741 tgtaatgcaa ttatcttttc tgcctccgga atcattgatt tatccacacg gtttaaagga
     7801 acttctatat tatgctggtc aaagcttatc tgccccgatg atacaagagt agtttttgta
     7861 tagatttgaa gtactgagcc agctatgcac cttcccatat gctttgaatg ctcgtagccc
     7921 ctattcgctt tatcaagagt agtaaataca tttatatggt ttgtatcacc ttgtgcacca
     7981 ttaaagtaaa tacaatgtac attatccagt gctttttcaa gagtatcacg aacaaattta
     8041 ggataatcgg cagaaaattt attaccgcct atcacatcag gatgcacctg aaaattaaca
     8101 agtagtattt cctttgcatt atcacgatgc aaacgcacca actggaccgt ttcatcagga
     8161 gttccaatcg gttccagaat attctgatta ccaacacctg gattagtctg gattttgcca
     8221 tctttcatgc ggaagcggcg tacaaaagaa ataccaggtg cagtactgcg tccaatactt
     8281 acagatgcag gctttaggtc ttctattgcc atagttgtca catcacttaa ccgcctttga
     8341 agataatcgt tgtagttggg atcctcagga aacaaacggc tctgtaatac aggcccggtg
     8401 tgcgtatggg tacacgcaat aaatactgcc tcaagaggta tggaattacg ctcagctatt
     8461 gactttcggt acacattcat ctttttctgc ggaatgccta taagatctgc actaattaca
     8521 acagctgtat tggaaccatc actgacagct acggctgaag catataaatt atcaagtatg
     8581 ccatcagcta tacgttggtt gtaataacca gcaatactga tcccaaatgg cggtgttata
     8641 tcaagacgtg cataacctgc tttaaaattg ctcatactat attgcccccc ttattataat
     8701 gcatccttta ttataatgca tctttcaaat aataacctgc ttcatatata ctacaatata
     8761 tttattcatt ttcaaagtga caattttcgg tatgagtgac aaattcgtca attttatagc
     8821 tgtaatccct taacatataa tcttctaatg caattgtctt gtaaatacca gtaatatatg
     8881 tcggtgtaac caatttaacc cacttgtttt aactttttaa aacccataaa actttttaag
     8941 agtattatca tccaattttt ggatgttata attaaaatat tcaatatggt ctttctattt
     9001 tgacatcctg cattggaaaa tgttttaaac ttaaagtaaa caaacaggcg tctaaatttc
     9061 ttgctgatgc tgcaattata gcatctggta cattaattcc atgacttttt atatatttat
     9121 tcatatataa cccagcagaa tatgcaattt ccttcgaaac atcaacttct ttgaatgctt
     9181 ctaaaagtcc acttattatt gactcttcgc tttgccgcat tcctgtcatt aattcaatac
     9241 gagtaatgac agaataatat acagaatcat atttattaat acagctcttt agaagttctg
     9301 ttgcctcatt cctgcctcta agatgatcta caaatatatt tgtatcaatt aatataaaca
     9361 ttatctattc atacctccgt atctgaaggt gttatattcc atttttccct catttcatta
     9421 acataactat ccgaatcagt tatatcagtc ctatcagccc ataatccttt agtatttatt
     9481 attttttttt cagattcttc attgcttttt gcaagatact ctactactgc ctcacgtacc
     9541 agctctgcca ttgtggttct tttttcctta gccatctttt gtaattcttg tttatgactc
     9601 cgatcaagat aaacttgtgt tctttccatg tatacatcac ctttctgcta taatgtatac
     9661 atatatgata tacatttgtt ttgaaaatgt caactacttc catgcaaaaa ttttattcaa
     9721 taattcttaa agttgtgatt tattaatttt gtggtatatt tttgttcata agatgtttgc
     9781 tagaaaggta gagaaatgaa aatatattat caatactatt gcagtgtcag cagagtagaa
     9841 aatccagtta tctggttaat ggaggtatta aataatgaaa attataatta aagatcctaa
     9901 cacattaagg cgtgctggaa ttgaatcgct tgcaaaaaac cttggtccgg taggtgcggt
     9961 atacttccta cgtcaatacg atattggaga agatgattac accaaggaac gcaaagaatt
    10021 actaaagatt ataagtattg aagatgtaaa aaaggatatg gaaaatttta aagattctta
    10081 aagtctgcat ctttttttac tgccttttaa aaaggttcct aaacaaattc ccagaactaa
    10141 tatctgttca ggaacctttt tatattataa tcaaaatgtc ttatatgttt acaaatacca
    10201 aattcttgtt ttcttcactt cataatctca accatactag aatagtattt aaggattaga
    10261 ggcatatcat attcataata agtttgatag catgcctgaa aatcagtttt tgaaggatca
    10321 tcagccactg caagccacat cttcaggtct tttggtataa aaacagcatt atggaggttg
    10381 cttttcattg atacaggtct tttaagaatt tcaattaaaa tatccttatc aatatttcca
    10441 tagttttctt ttaccctttt caatgcctcc gtataacgtt cttttgctga agctatcaaa
    10501 gcatcttttg gtggaggtga cggcaggaaa ggatgattct gtcccggctt aacagtcatg
    10561 aatgtttttg gagtgcaata cagaactctt gcatctttaa ttttcgcatc ggaaattaca
    10621 taaaaatatt cacatgtacg cgggtttttc ttaaaagtat ttacactatc ttttagtttc
    10681 tctgtttcct ccagtgccat tctcatcaaa aaagtcatag gtatgccttc ccattgtcct
    10741 tccccgcctc ccccgatttc gccaacagaa atctgcttat cattcattcc cgtaacactt
    10801 cctattaaac ccgccatacc tgcattaaaa aatgtattat atccatcagg ctgcattaac
    10861 ataacaattg agtgattctg tataccttgc tcagttgcat aatcaagaat ccttgcatgc
    10921 aataattccc catcctttgt tgcttttccg aataatacaa ttccactgca atgaaataac
    10981 tctgggaaca tattcaccaa ttgagcatct tccagtttta tttctgcacc ttctgcaaga
    11041 ccttttaatt cttccttata ccgctcaggg atatactttt ctgccatatc ccaggctttt
    11101 tttagttctc caggctttat ttctgatatt ttctctatta ctatctttgc aagggctgca
    11161 acctcgtctt ttagtaaaac tccatgctga tatcccattt cataaggtgt ccctttaaga
    11221 acaattattc tttgaccgtc cttttcaagc aaggctccct ttccttcccg ggcaattaat
    11281 gatatacctg cttttttaag ggcttcatca gaaatgttag ccgctaaaga acttatgctt
    11341 ttaggatttg cactctggca agaagtaaga gacattacaa gaaataaaga gagaatagac
    11401 atggccgtag tcttagcagc tgttttaact gcagttttgg aaatacagat attttttccc
    11461 attgtacaac ctcctcatat tggttttaac tgcttacttg acactactca taattataat
    11521 aaaaaactac taaatacttt gttttttaac tataagtact ctaactccca tttataactt
    11581 aatttgcagg tgtcctcaat agttacataa aaaatgatat tcggaacaag catattggaa
    11641 acctttatga taattatgac gaggcgttag atgtccccta cctctgcagg tggtgggtaa
    11701 cgctttagtt tttaggcggt gagcatatct cacaccttgt tgtaatgcgc aaaggttgta
    11761 aatattttct actgatgtgt tataacacac atgcttaatt tttacaatta aggaaatcgt
    11821 acagtgtgca ttttccgtat cacaaaaatc agcacctaat aaaattgtac tgattttagt
    11881 tgtactacga gagatattaa acatatctat taatttttat gctttgtcag tatttttaga
    11941 atatgagtat cataagttgc agcaatcttt tcagaaatta tcgcttttat agatactgat
    12001 actacttgat ttaaagtttt tgctaagtgc tgtttcccta ttttaaacga tatcctagtg
    12061 gcagtttcag aatatttctg aaactgccgc ttctttatta accttctttt gtttctcttt
    12121 attgtattat gtctagcaga ttttttatgc tttttgcgca ttctgcacgt gtagcagatc
    12181 ttttgggttc aaatgttccc ttatctgttc ctatcataag tttcatttcc gatacagttc
    12241 ctactacaga tctagcccat ggacttattt cagctgaatc cttaaaggcc aattcaacat
    12301 cttttagaac tttagaacct gttaataatt cgtacgagcg taatataata gctgcaattt
    12361 cttcccgact aatatttcta tcgggataga atttttcatc ctcagtacca gttactaagc
    12421 ctgcctttgc tgctccggat attagacttg aataccagga accttctttg acatcactaa
    12481 acgcaattgt gctgctttcc tcaataccta aagctctaga tatcatagca ataaactctg
    12541 cacgtgtcgc ttctttatta ggtgaaaact cagtaagact agtcccttta attataccct
    12601 ttgcagccat ttctttaata acatcaatag cccagaattc ttgcagaaca tctgtaaatc
    12661 gcttctcata agtcaataca gcaaatgtaa ttgggtaatt aacttctgcc tctatagtat
    12721 catcttttat ctttccacct aaatgtttta tttgtccatc tacagtaaaa ttgtaaatat
    12781 atgttagttt tgttgcttta tttctaggtg tttttaagga aacagttaca ggtaaaggtt
    12841 ttaagttatt aatcttttga ttgttttcat ctaaaacatc tagttcaata aaccacatat
    12901 ttcctattag ctccagttca acttcttctt tagcctctac attattaagc agtttttctg
    12961 ctatgtctgc tgttaactta tctgccttta cttcaatatt atatttatca gctttatcag
    13021 atgatatttg atgtaataca ttacttatcc atggataagt taatattatt tcgattgatt
    13081 cgttagttat tactatatta ttaccttttt catccatatc atcaacaatt cctagtggta
    13141 ctgttacttt attttggtca ggctggaatt caaatatttt attaccttca tcatcaactc
    13201 tataatcttc ttccccagct ttgtacaatc ctacatcatc aatatacaat acttcatctc
    13261 ttctaatgtt ctccactttc actataatgt gtagaatttt taccgcttca ttttctatat
    13321 tcaactctct ttctatagtg tgccattttc ctgcggttgc acttaatcta tatctagtac
    13381 cttcaggaag ttcttctatt atatttccat ccttatcctt ataataagca tgcagactta
    13441 ttcgtccaac agtgtttgtt ccttctgcta tataatacga agctctgaat ttatattttc
    13501 caggttccat ttggcttatg ttcatatgag gtccaccaac ggtcaaaccc gatattttta
    13561 aactgtaatc atctgttttt gggttatctg tgctgtattc cattacagca ccctcatgtg
    13621 tccagaatgt ccataggttc ctttcaggtt cttcaaaaga tgagcctttg ttaagtggtc
    13681 ggtttggatc gggcttttcg tttttctctt ctccaaccct acacaatcct acatcatcaa
    13741 tatacaatgc ttcatctctt ctaacattct ccactgtaac tattatttgg atgtatttta
    13801 tatcttcact ttcaatatac agatctcttt ctattgtgtg ccattctcct gctgttgcac
    13861 ttagtttgta tctagtatca tcaggcgctg ttcctatttc cttcccatct ttatcccggt
    13921 atattgcagc gaggcttatc cttccaattg tatttgtacc ctctgctata taataagagg
    13981 ctgtaaattt gtaccttcct ggatctattg gcgttacatt catgtgaggt ccgccaattg
    14041 ttaatccaga tatttt
//
LOCUS       DAOSDD010000106        14050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1706_length_14050_cov_5.326617, whole genome shotgun sequence.
ACCESSION   DAOSDD010000106 DAOSDD010000000
VERSION     DAOSDD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_14050_cov_5.326617"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK733_10525"
     CDS             complement(<1..334)
                     /locus_tag="PK733_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOJ11012.1"
                     /translation="MYKVGFIGLGRMGSGICNNIIKNGHDTVVFDTNPEAYRNIEERM
                     HAAKDVSEVFSLCEITFLSLPGSIQVEETIGKFMECGVEGKTVIDLSTSYPISSRKIY
                     ELFKNNGGY"
     gene            complement(441..1817)
                     /locus_tag="PK733_10530"
     CDS             complement(441..1817)
                     /locus_tag="PK733_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ11013.1"
                     /translation="MEYISFDPQNVKVVGEYDLIIVGGGTAGAIAAISASQEGVNTLV
                     IEQFGALGGSGTNGLVTPVMPSGVCGNPEYCSISKKIFNKMESYGFAIEDIEESWHKD
                     WFDPMMMKFVLEELCVEAGCNILYYTVLVDVIKEEEKIKYVIVKNKNGLSAYSAKCFI
                     DCTGDGDLAYLSGVPMESGNHKGLNQAVSLRFQMTNIDLERFGLYLESIGQKKDTRYP
                     FLHTAAVTGGKWPLYELFLEKFAEGYLGKTEITYFQAFSLPGKPRDLAFNCPELGQNK
                     NVIDAWYMSRKQIEGKKSIMRLTAFLKKFVPGFEEAYLCDVAAMLGVRESRRIISDYY
                     LKTEDLLSYKKFADGIAKSNYPVDIHGDEGAEGLVYKDISDNEKYYEIPFRSLVPVKI
                     ENLVVAGRCMGADFIAQSSTRVQHTCRATGEAAGIAAVLFIKEGTIFRNIDGSKIRNI
                     MVSRGVDL"
     gene            complement(1862..2566)
                     /locus_tag="PK733_10535"
     CDS             complement(1862..2566)
                     /locus_tag="PK733_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ11014.1"
                     /translation="MRIMAFAAHPDDELTCAGTLAKYKDNGHDVGIVIATNGEVGSST
                     LSKQEIAALRKKEAENSASVIGAEFFWLNYPDEFLFNTKEVRLHFIDIIRGFKPDIII
                     CHDKDMDYHPDHTTTGQIVWDVHVMVTVPNIKTKNPPCITIPEIYFMDTAAGVNFQPE
                     FYVDISGQWDKKAKMYSCHTSQEEWMLSQYNVSFVEFGRIQAIYRGFQAGSIYAECFR
                     KAKFFPGGVKVNSLLL"
     gene            complement(2579..3682)
                     /gene="menC"
                     /locus_tag="PK733_10540"
     CDS             complement(2579..3682)
                     /gene="menC"
                     /locus_tag="PK733_10540"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOJ11015.1"
                     /translation="MYIDAVNVYYLKMPLVTPWRTAYGEDAYMHTVLVCLNSSGKKGW
                     SETGPLVTPTYSPEYTYGVYYLLTEFFAHHIKGREISSPDRLLELLSQYKGNPFAKAA
                     LETAWWTLESNVKGVPLYKLLGGSLKEVEVGADFGIHDNINELILKMERAYSSGFKRI
                     KLKVKPGKDNSVLKEVRKHFPDQIIHVDCNSGYTLKDLPVFIEMDKYNLHMIEQPLAY
                     NDLHDHAVLQSKINTPVCLDESINSVKAAREALELKSCKLMNIKTGRVGGLKNSVEII
                     ELCKEKGVGCWVGAMLEAGIASGAGIAISTLDNIVYPGDIFPSGTMCREEISKVEIKL
                     SSPGHMKPLDFPGFPYEPREDILKKYTVFSKTI"
     gene            complement(3775..6504)
                     /locus_tag="PK733_10545"
     CDS             complement(3775..6504)
                     /locus_tag="PK733_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11016.1"
                     /translation="MMVTKAKNEVPTKKEVSIVWKKGIAEGTVEIINGRLLSIITMRD
                     SVITAEKIIDGTGPVKNKDNTASYRLCDADSRLIIEAEGNIDLITEGAARITINNFEN
                     PFTFILGDISNEYPVYIPQYGVIITTGEDNRSYSEIENAILAKGLKKELQLLDEEKEE
                     SFISADEATRDLSCMTWLGLSRDVRTFWIGFRGSVDKEKRVYDYIIPKYHNSGVKPPE
                     LDKDSQFTILTGRGFGCSENVTRWLEDGYLPILRAKIEDGNITYNITSFTAPEISDFT
                     KEKLRGTHCIAADGYSAGHMFTEEQQQLFDSLGEKEVNGEEQVILYYRIEAVNNSKMP
                     DYAHFIAPVPSHRIGMLEETEYNFDEEKGYGRYKSGRVFSISRINGQILRKPEFSTLL
                     NPGGKAVYDFLIPHSPLEGDRADNLSKADFNKSLAQCKDFWKEKLKEAAQFTIPEKRI
                     NDMVKAGICHIELIAYGNEPNGSITPTTGIYSAIGSESAPIILFMDSMGYHNTAKRMI
                     QFFFDKQHDDGFIQNFANYMVETGPVLWCAGEHYRLTLDKEWAKSISVNLVKACNFTI
                     NWINKNKQEKYKGRGYGMIDGRVGDPEDPYHSFMLNGYAYLGMARAVEILEAINHCEA
                     SRIRKETDEFKKTIRESFYDNLKKSPVVPLDDGSWCPTAAPWPENHGPLAFHFEDGKW
                     ITHGGLVNRDSLNGPLYLVMTEVLDINEPVVEFLVDYHSRYMCMRNVGFAQPYYARHD
                     WVHIKRGDVKAFIKAYYNCFAGMSDKETYTFWEHYFHASPHKTHEEAWFLLQTRWMLY
                     LEDRAALKILPGAPRAWLKDGNIIDIRGVASYFGHLDFRIEAKLNSKCGTNCIIAEIK
                     CEKGRLPEILEVRLPHPDGLKAVKCSGAEYIPEKEKAIVYDFNGEAKIVLTFE"
     gene            complement(6510..7412)
                     /locus_tag="PK733_10550"
     CDS             complement(6510..7412)
                     /locus_tag="PK733_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11017.1"
                     /translation="MHRYAKKGIKNLSIFDCINIIIMLFVMIVTVYPFYYCIVLSFND
                     GKDALFPGIYFWPRMFTLDNYAFIFNNKQLLFAFKNSILRTIIGTAIAVIITSAGAYA
                     LTKKHLIGRRFYLIFFILTMYFSGGMIPTYLVYRKLGLIDNFLVYILPPAWSYYNALL
                     CMAFFESIPDSLEESAFIDGAGPIYIFIKIIFPISTPIIATISLFIGVWHWNYWFDTM
                     IYTTRSDLQTLQNLLMIVIREAENMIKLSQQAAQLGAVAVFNVTPVTVRVATMIVTTF
                     PIIIVYPFVQKYLIKGIMLGAVKG"
     gene            complement(7428..8345)
                     /locus_tag="PK733_10555"
     CDS             complement(7428..8345)
                     /locus_tag="PK733_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11018.1"
                     /translation="MYFRKFIKKFRKQSAYHLMLFPGFLAALVFSYVPMFGIIIAFKD
                     FSLRRGVFASKWVGFSNFKHFFTDMGLMPAIINTVAIGFLSIVLAFPAAIIFALLLNE
                     LKSRKFKRLIQTVSYFPHFISWVVISVIMITFLSPSEGIVNHIIVSLGISDNPILFIG
                     KPEYFWWIAVLSVIWKETGWKAIIYIAAISGIDQTIYEASLIDGAGRFKRIWYITLPS
                     ISGTISILLILSISSIPFVAFDQSYLLGNTLNYERSIVLSHYVYKLGIINGNYSYSSA
                     VGLILSVVSAAFMIAANYISKKTKGTGVV"
     gene            complement(8417..10195)
                     /locus_tag="PK733_10560"
     CDS             complement(8417..10195)
                     /locus_tag="PK733_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11019.1"
                     /translation="MSKNLKMSIILFVILALIGTMSGCSTAGKDTKDEKASTTAATKD
                     ATQQTTTAAQTVSDKPYVWKPDPADLEWKDDTSPVTLTAFLNFPSTIPFEWGKNDVTK
                     EITKRTGVTIEATYAPDNESQRLSVMLASGDKLPDFIIGPKPPDPLYYNMIKDDVIWS
                     YTELMDKYAPNMRKVMMSGNEEICAEDDGKLYWLGMTMVGYDQTDDIRIAPPPKFAVR
                     NDVWEALGKPQIKTTDDLLDVLRKVKANNFEGIKYPIFLDVYNNIPNGTMFGVPGSQN
                     GWIYDYENNKVNYWTELPKGKESLRFINGLVREGLFPEEAFTVQNYNDEIDKGSIFLL
                     GNQNIFYVYGANKRLSESNPAKYYRYIEPIAKENEEFNILFNFRAYSWRGLTITKDCK
                     NPERAIKYIEYMISDEGQQLFLYGIDGVHHKMKKTEYGWSYPVFNDDLSKLISTDFLS
                     LGSKYGVYAHLTHCWLIKDIYDYNFVYGLGFSEDNKLKREGEMAYLKYRTPNRVDAIP
                     NSVPSPSTDTLKPIPTKITEFNKQQFGKLLSAKTDEDFEKEYKVLIDGYSKVGIESLK
                     EYYVKECKQQLEIAKNTRLNINYPTK"
     gene            complement(10466..12223)
                     /locus_tag="PK733_10565"
     CDS             complement(10466..12223)
                     /locus_tag="PK733_10565"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11020.1"
                     /translation="MVKRIVKGLINNIRIKYKFIIFFSFIFFLFISFTTYILFTRSYS
                     LIVEKKNETYLQNINQTNRNIVFTLNSYEKILNTFFLNKSFRESLVQEFANIAEQLDF
                     QTWVYNYVDSLTLNYEPLPSIKIYTGNESLKVDNRMFFSLNEFKSTEWYNMVHNRPER
                     INIHRWLNTIKRRKHESDFYIICGLVEILDVYTKEKIAVITIEIDVKQLFSFLDTPNN
                     KLVITDDRGNIIYSNDSDIITTNISANEYFSVIKNEENGSFVTKIDGNSMMIFYNTIN
                     PMGWRLINIVEESFFLNETLAVRKFAIFLAILGIVTSIIITFVYVTIITKRIMILTKA
                     MTEVGKGNFEVSVSIAGSDEIGKMNDVFINMVQDTKKLIEKVRTSENSLYRFEIMSMQ
                     EQIKPHFLYNTLSAIGLMAIDIEAYDIYSALLSLSEYYKISLSNGDIIIPIKNEIIHI
                     KAYVNLLKLRFANKFNVDYFIDESVYEYYTPKTILQPIIENSVIHGFYNKKSETNLIR
                     IKVFRKNDNIIYEISDNGVGIAEDRIKEIFNSNSESFALKNIDSRIKLICGNKYGLDI
                     TSIPGEGTTTNIKLPLINK"
     gene            complement(12247..13878)
                     /locus_tag="PK733_10570"
     CDS             complement(12247..13878)
                     /locus_tag="PK733_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11021.1"
                     /translation="MIKLFLVDDEVSDRDLIVRGINWDDIFIELVGYAANGEDAIQLI
                     RNTRPNIVLTDISMPVKNGIHLANLLKEEFPDIKVIFISGYQEFCYAKAAIEYNVVHY
                     IVKPVNIFSLSNLLSDVAKQCIEENKKKFEMEIFKLQLEQSLPLLKESFFREWIFGSF
                     KDEDILKRKLDFLNIPFIVPNKSEKNVETIVIVLQLDDFKDKYDYSEYEKQLLNARVL
                     DCINDAIERYGISYCFSSKFGEYVIIIQNQSGCDSNFNEEVISKTCDIMRRNIELSCM
                     QTCTIGIGGFFYNLVSVPDYYKKCIDAIQYKFYYGANQIFFYNDVKLIGISGFDSENY
                     KLEIIKFLKIGDGRSISNLLDEILGITSYSQYFNIHYIKNLSIEILSACLNTINEYPT
                     IAESDLKDITDIVAKLEKFGSIDSIWNWLKKIILEMSFKIYSNVKNKNAIIVKHIKEI
                     ISAKYDSIVSVEQIAEDINLSPGYASTVFKNETGETIIQYLTYVRMQNAIKLLEDTNL
                     MVFEIAQRIGYNNTTHFASVFKNNIGISPGEYRKK"
BASE COUNT         4344 a         2747 c         1981 g         4978 t
ORIGIN      
        1 aatatcctcc attattttta aaaagctcat aaattttcct gctagatatt gggtagcttg
       61 tgcttagatc tataactgtt tttccttcaa ctccgcactc cataaacttt ccgatagttt
      121 cttcaacctg tattgaaccg ggcaaactca gaaatgttat ttcacataag ctaaatactt
      181 cacttacatc ctttgcagca tgcattcttt cttcaatatt cctatatgct tcaggatttg
      241 tatcaaatac aacagtatca tgaccattct taataatatt attacatatt ccacttccca
      301 ttctccccaa gccaataaaa ccaactttat acactaaaaa cacctccaat taaatgttag
      361 ccgttatata cccagtaact tccagtttat aacatatgcc ttagctatat gctagtttta
      421 aatgtaaatc gtcacctaaa ttataaatcg acaccccttg aaaccattat gtttcttatt
      481 ttacttccat caatatttcg gaatattgtt ccttctttta taaacaggac agcagcaatt
      541 cctgcagctt caccggttgc gcggcaggta tgttgtacac gtgttgatga ttgtgcaata
      601 aagtctgcac ccatgcatct tcctgcaaca accaagtttt ctattttcac gggaacaagg
      661 cttctaaaag gaatttcata atacttttca ttgtcagata tatccttata aacaagccct
      721 tctgcaccct catctccatg tatatctaca ggataattac tttttgctat tccatcagca
      781 aattttttat aactaagcag gtcttctgtt tttaaatagt aatcagatat tatccgcctg
      841 gattctctta ctccaagcat tgccgcaaca tcacataaat aagcctcttc aaaccccggt
      901 acaaactttt tcaggaacgc ggttagtctc ataatagatt ttttcccttc tatttgtttt
      961 cttgacatat accaggcatc aataacattt ttattttgtc caagttcggg gcagttaaaa
     1021 gctaaatctc tgggtttgcc cggtaaagaa aatgcttgaa aatatgtaat ttctgttttt
     1081 cccaaatatc cttctgcaaa tttttctaag aacagctcat aaagtggcca cttcccaccc
     1141 gtaacagctg cagtatgcag gaaagggtac ctggtatcct ttttttggcc aatactttcc
     1201 aggtatagtc caaatctttc aagatcaatg ttagtcattt gaaaccttaa agaaacagcc
     1261 tggttcaagc ccttatgatt acccgattcc ataggaacac cgcttaagta tgcaaggtct
     1321 ccgtcgccgg tgcaatctat aaagcatttt gctgaatagg cagataaacc atttttattt
     1381 ttaactatta catattttat tttttcttct tcctttatta catcgacaag tactgtataa
     1441 tatagaatat tgcagccagc ttcaacacaa agttcttcaa gcacaaattt catcatcatc
     1501 ggatcaaacc agtctttatg ccaggattcc tctatatcct ctatggcgaa tccataactt
     1561 tccattttat taaaaatctt tttgctaata gaacaatatt caggatttcc acatactccg
     1621 gatggcataa caggagtaac caacccgttt gtccctgaac cgcccagagc accaaactgc
     1681 tctattacta aagtatttac accttcctgt gaagctgaaa ttgctgctat tgcacctgca
     1741 gtaccacctc caacaataat caaatcatat tctcctacaa ctttaacatt ttgtggatca
     1801 aatgaaatat actccatatt tatctccatt tctgcaagga ataaattaat tacaccttgt
     1861 cttaaagtaa tagactattt acttttacac cgccggggaa aaacttagct tttctaaaac
     1921 actcagcata tatactacct gcttgaaacc ctctataaat tgcctgaatt ctgccaaatt
     1981 ccacaaaaga tacattatat tgcgatagca tccattcttc ctggctggta tgacagctat
     2041 acattttagc tttcttatcc cactgtccgc taatatcaac ataaaactcc ggttgaaagt
     2101 ttactcccgc tgcagtatcc ataaagtata tctccggaat agttatacat ggtggatttt
     2161 tggtcttgat attaggaaca gtcaccatta catggacatc ccatacaatt tgtcctgtcg
     2221 tagtatgatc cggatggtaa tccatgtctt tatcatggca tattataata tcaggcttga
     2281 atcctcttat gatatcaata aaatgcaatc gtacttcctt agtattaaat agaaattcgt
     2341 cagggtaatt caaccagaaa aactcagccc caataacact tgcagaattt tcggcttctt
     2401 ttttccttaa tgctgcaatc tcctgcttgc tcaaagtcga tgatcctact tcgccatttg
     2461 tagctattac aatccctaca tcatgaccat tatctttata ttttgcaagt gtgcctgcac
     2521 atgtaagttc atcatccgga tgagctgcaa aagccataat tctcataaac attacctcct
     2581 aaatcgtttt agaaaaaact gtgtactttt taagaatatc ttcacgcggt tcatatggga
     2641 atccggggaa atcaagaggt ttcatatgtc cggggcttga aagttttatt tcgacttttg
     2701 atatttcctc acggcacata gtaccggacg gaaatatatc acccggatac acaatattat
     2761 caagtgttga aattgcaata ccggctcctg aagctatacc agcctcaagc attgctccga
     2821 cccaacatcc gacgcctttt tctttgcata gttctataat ttctacagag ttttttaaac
     2881 ctccgaccct tcctgtttta atattcataa gcttgcatga tttaagttca agcgcttctc
     2941 ttgccgcctt taccgagttt atgctttcgt caaggcaaac aggtgtattg atttttgatt
     3001 gcaaaactgc atgatcgtgt aaatcattat atgcaagtgg ctgttctatc atatgtaaat
     3061 tgtatttatc catttcaatg aaaacaggta aatctttaag ggtataacct gaattgcagt
     3121 caacatgaat aatttggtcg ggaaaatgct ttctcacttc ctttaaaaca ctattatcct
     3181 ttcccggttt tactttcagc ttaattcttt tgaagccact agaataagcc ctttccattt
     3241 ttaatatcaa ttcatttata ttatcatgta ttccaaagtc tgcacctact tcaacttctt
     3301 ttaaagaccc tcctaataat ttatataatg gaactccttt cacattgctt tcaagagtcc
     3361 accaggcagt ttcaagagct gcttttgcaa aaggatttcc tttatattgt gacaaaagct
     3421 ctagaagcct atcaggtgat gaaatttctc taccttttat gtggtgtgca aaaaattctg
     3481 ttagcagata atatactcca tatgtatatt ctggtgaata agtgggtgta acaagagggc
     3541 ccgtttcaga ccatcccttt ttaccgcttg aatttagaca tacaagaaca gtatgcatgt
     3601 aggcatcttc accatacgct gttctccagg gtgtaactaa aggcattttt aaataatata
     3661 catttacagc atcgatatac atctaattac ctccatctgc aatatccatt atttgctgca
     3721 ctatacggta ttttagcttt attatttgaa tactatacag ttgcagcagt aatatcattc
     3781 aaatgttaaa acaattttag cttctccatt aaagtcatat acaatagctt tttccttctc
     3841 gggaatatat tctgcgccgc tgcattttac agcttttaac ccatcaggat gtggtaatct
     3901 aacttcaaga atttcgggca atctgccttt ttcacattta atttcagcta ttatgcaatt
     3961 tgtaccacat ttactgttaa gcttggcttc tattctaaaa tccaaatgtc caaaataact
     4021 tgcaacacct ctaatatcaa taatattacc atccttgagc caagctcttg gtgctccggg
     4081 taaaattttg agcgcagctc tatcttccag gtaaagcatc catcttgtct gcagaaggaa
     4141 ccatgcttct tcatgagttt tatgcggact ggcatggaaa tagtgctccc agaaggtata
     4201 ggtttcttta tcggacattc cggcaaaaca attataataa gctttaataa atgcttttac
     4261 atcaccacgt tttatgtgaa cccagtcatg cctcgcataa tagggctgtg caaaaccaac
     4321 atttctcata cacatatatc tggaatgata gtcaactaaa aattccacca ccggttcatt
     4381 tatatctaaa acttccgtca ttacaagata taaaggccca tttagagaat cccggtttac
     4441 aagtccgcca tgtgtaatcc atttaccatc ttcaaagtgg aaagccagag gtccgtgatt
     4501 ttccggccag ggtgctgcag taggacacca actgccatca tcaagaggca caacaggact
     4561 tttctttaaa ttatcataaa aactttctcg aatagtcttt ttaaattcat cagtttcctt
     4621 cctaattcta gaagcctcac aatgattaat agcttccagt atttctacag cccttgccat
     4681 acccagatat gcatagccat tcaacataaa agaatgatat ggatcttcag gatctcccac
     4741 cctcccgtca atcattccat aacctctccc tttatacttc tcctgtttgt tcttgttaat
     4801 ccaattgata gtgaaattac atgctttaac aagattcaca cttatactct tcgcccattc
     4861 cttatcaagt gttaaacggt aatgttctcc ggcacaccac aataccggac cagtttcaac
     4921 catataattt gcaaagtttt gtataaagcc atcatcatgc tgcttatcaa aaaagaattg
     4981 aatcattctt ttagcagtat tatggtatcc catggaatcc ataaataata taataggagc
     5041 actttctgat cctatagcac tatatattcc ggtggttggt gtaatactcc cgttaggttc
     5101 atttccataa gcaataagtt caatatggca aatacctgcc ttaaccatat cattaatacg
     5161 tttttcaggt attgtaaatt gtgcagcttc tttaagcttt tccttccaga aatctttaca
     5221 ttgtgccaga cttttattaa agtctgcttt agataaatta tccgctctat ctccctccaa
     5281 tggactgtgg ggtataagga aatcatagac agcttttcct cccgggttta ataaagtgga
     5341 aaactctggt tttctgagaa tctgtccatt aattctggat atgctgaata ctcgtccact
     5401 tttatatctg ccatatcctt tttcttcatc aaaattatat tccgtttctt caagcatacc
     5461 tatcctgtgt gaaggaaccg gtgctatgaa atgtgcataa tcaggcattt tactattatt
     5521 tactgcttca attctataat ataatattac ctgttcttct ccattaactt ccttttcacc
     5581 caaactgtca aataactgtt gttgttcttc agtaaacata tgtcctgcgg aatatccatc
     5641 tgcagcaatg caatgtgttc ctctgagctt ttcttttgta aaatcagata tttccggagc
     5701 agtaaacgaa gtaatattat atgtaatatt accatcttct atttttgctc tcaatatagg
     5761 aagataccca tcttccagcc atcttgttac attttctgaa caaccgaaac ctctacctgt
     5821 cagaattgta aattggctgt ccttatctaa ttcaggcggc tttactcctg aattatgata
     5881 tttgggtatt atataatcat atactctttt ttctttatca acacttcctc tgaaacctat
     5941 ccagaaagtc ctgacatctc tgcttaggcc cagccaggtc atacaactta aatcgcgtgt
     6001 agcttcgtca gcagaaataa agctttcttc cttttcctca tcaagcagtt gtaactcttt
     6061 ttttaaacct ttggctaata tagcattttc aatttctgaa taacttctat tatcttctcc
     6121 tgtagttatt atcactccat attgaggtat atatacaggg tattcattgc taatatcacc
     6181 aagtataaaa gtaaatggat tttcaaaatt attaattgtt attcgtgcag ctccttcggt
     6241 tattaagtca atattacctt ctgcttcaat tattaacctg ctgtctgcat cgcaaagtct
     6301 ataacttgct gtattgtctt tatttttaac aggaccggta ccgtctataa ttttttcggc
     6361 tgtaatgaca ctgtctctca ttgttattat cgacagtaga cggccgttta taatttcaac
     6421 agttccttct gctatgcctt ttttccatac tattgatact tctttttttg ttggtacttc
     6481 attttttgcc tttgttacca tcattatcat catcctttca cagccccaag cattattcct
     6541 tttattaaat atttctgaac aaacggatat actattataa ttggaaatgt tgtaactatc
     6601 atagttgcaa cccttactgt aacgggggtc acattaaaaa ctgccactgc cccaagttgc
     6661 gctgcctgtt ggcttagctt aatcatgttt tctgcttctc tgattacaat catgagcaga
     6721 ttttgtaatg tctgcaaatc tgatcttgta gtgtaaatca tagtatcaaa ccagtaattc
     6781 caatgccata caccaataaa taatgaaatg gttgcaataa tcggtgttga aattgggaaa
     6841 attattttta tgaaaatata tattggacct gcaccgtcaa taaatgcaga ttcctctaat
     6901 gaatccggaa ttgattcaaa aaaggccata cataataaag cattataata actccaggca
     6961 ggagggagta tataaactaa aaaattatca attaggccaa gcttcctata tacaagataa
     7021 gtaggtatca tgcctcctga aaaatacatt gtcaagataa aaaagattag gtaaaacctt
     7081 cgaccaataa gatgcttttt agttagagca tatgctcctg ctgatgttat tattacagca
     7141 atagcagtac cgataattgt cctcaatatt gaatttttaa atgcgaataa caactgcttg
     7201 ttattaaata taaatgcata gttatcaagg gtaaacatcc tgggccaaaa ataaatgccc
     7261 ggaaacaatg catctttacc atcattaaag gacagtacaa tacagtaata gaatggatat
     7321 accgtaacta tcataacaaa taacataata attatattaa tacaatcgaa aattgataaa
     7381 ttttttatgc ctttttttgc atacctatgc ataatctttt atacccccta aacaaccccg
     7441 gtacccttgg tttttttact tatatagttt gctgcaatca taaaagctgc tgaaactact
     7501 gataaaataa gtcctactgc agatgaatac gagtaatttc catttatgat gcctaactta
     7561 taaacatagt gtgaaagcac tattgaccgt tcataattca atgtatttcc caaaagatat
     7621 gattggtcaa aagccacaaa tggtatgctg cttatactta aaatgagtaa tattgatatt
     7681 gtcccgctaa tcgatggaag tgttatatac caaatacgtt taaatctacc ggcaccatca
     7741 attaatgatg cttcataaat agtctggtca attcctgata ttgctgcaat atatattatt
     7801 gctttccatc ctgtctcctt ccagataact gataatactg caatccacca aaaatattcg
     7861 ggtttcccaa taaagagtat tggattgtct gagatgccca aagatactat tatatggttt
     7921 acaattcctt cagatggtga caggaaggta atcattatta ctgatattac gacccacgaa
     7981 ataaagtgcg gaaaataact tacagtttgt ataagccttt taaatttccg gcttttcaat
     8041 tcatttagaa ggagagcaaa aataatagct gcaggaaatg ccagtactat actcaggaaa
     8101 cctatagcta ctgtatttat aattgccggc attaaaccca tatcagtaaa aaagtgttta
     8161 aagttgctaa aacctaccca tttgctggcg aatactccgc gtctgagtga aaaatctttg
     8221 aaagcaataa taataccaaa catgggtaca tagctaaata ccaaggctgc taaaaagccc
     8281 gggaaaagca ttagatggta agctgattgt tttctgaatt tttttataaa ttttctaaaa
     8341 tacatataca atgttacctc tgtttcaaat ggaaggtcgc tatactgcga ccttcccatt
     8401 ttattttaaa tgtaaactat ttagtaggat aatttatgtt tagtctcgtg ttctttgcaa
     8461 tttcaagctg ttgcttacat tcttttacat aatattcctt taacgattct atgcctactt
     8521 tagaatatcc atcaattaaa actttgtatt ccttctcaaa atcttcatct gtttttgcac
     8581 ttaacaattt accaaattgt tgtttgttaa attcagtaat ttttgtcggt atgggtttaa
     8641 gagtatcagt acttggactt ggcacagagt taggaattgc atccaccctg tttggagttc
     8701 tatactttag ataagccatt tccccttctc tcttaagttt attatcttct gagaaaccaa
     8761 gaccatatac aaaattgtaa tcatatatgt cttttataag ccagcaatgg gttaaatgag
     8821 catatacacc gtattttgaa cctaatgaaa ggaagtctgt agatattagt ttggacaaat
     8881 catcattaaa tacgggatat gaccagccat attcagtctt tttcatttta tgatgcactc
     8941 catctatacc ataaagaaaa agctgctgcc cttcgtcgct tatcatatat tcaatatatt
     9001 ttatagctct ttcaggattc ttacaatctt ttgtaattgt aagacctctc catgaatatg
     9061 cacggaagtt gaataagata ttaaactctt cattctcttt tgctataggt tcaatatacc
     9121 tgtaatattt agcaggattg ctttctgaaa gtcttttgtt tgctccgtat acatagaata
     9181 tattctggtt tccaagaagg aatatactgc ctttgtcaat ttcatcatta taattctgta
     9241 cagtaaatgc ttcttcaggg aataatcctt ctcttacgag tccattaata aatctgaggg
     9301 attctttgcc tttaggaagt tcagtccagt aattaacctt attattttca taatcgtata
     9361 tccagccgtt ttgtgaacca ggaactccaa acattgtacc attgggaata ttattatata
     9421 catcaaggaa tattggatat tttatacctt caaagttgtt tgcttttact tttcttaaaa
     9481 catccagcag atcatcagtg gtttttatct gaggtttacc gagagcttcc cacacatcgt
     9541 ttcttacagc aaattttggc ggtggagcta ttctaatatc atcagtctga tcatatccaa
     9601 ccattgtcat tcccagccaa taaagcttgc catcatcctc agcgcaaatt tcttcattac
     9661 cactcatcat tacttttctc atgttaggtg catacttgtc catcaattca gtatagctcc
     9721 aaatgacatc atcttttatc atattgtagt acagtggatc cggtggtttt ggacctatga
     9781 taaaatccgg aagcttatcg ccggatgcaa gcatgacact cagcctctga ctttcgttat
     9841 caggtgcata agttgcttct attgttacac ctgtacgttt ggttatttct tttgtaacat
     9901 cattcttgcc ccattcgaaa ggtatagtgc ttggaaaatt caagaatgct gttaatgtaa
     9961 ccggagaagt atcatccttc cattctagat cagcagggtc cggcttccat acatatggtt
    10021 tgtctgatac agtttgtgct gctgtggttg tctgctgtgt agcgtcctta gttgctgcag
    10081 ttgttgatgc tttttcatct tttgtatctt tgccggctgt actgcagcct gacatggtgc
    10141 caataagtgc aaggataaca aataagataa tagacatctt taggttttta ctcataaagt
    10201 tacctccttt attattttgt atttttgtaa ttatattgtt attatggtaa taattacatc
    10261 cattagtgca caaaatactc atgcggaaag ctttacaatt aaatctaaaa acaaataaac
    10321 ctgcaaacag ttaagtctat aacgcctgaa tagaactata caattattta accctgatat
    10381 aattatattt caatctttgt taaggagtca atttagattt tttcataatt atttagattt
    10441 tcttggatat tgcagatttc acacattatt tattaatgag aggcaattta atattagtag
    10501 tagttccttc cccgggaata cttgtaatat ctaaaccata cttgtttcca catataagct
    10561 taattctgct atctatattt ttaagagcaa aactttcaga attgctgtta aatatttctt
    10621 taattctatc ttcagctatt ccaactccat tatcactaat ttcataaata atgttatcat
    10681 ttttcctaaa tacctttatt ctaataaggt tggtttcgga ttttttatta tagaacccat
    10741 gaataacaga attttctata attggctgaa gaatggtttt aggagtataa tattcataaa
    10801 cactttcatc aatgaaataa tcaacattga acttattagc aaatctcagt tttaacaggt
    10861 taacatatgc tttaatatga atgatttcgt tttttattgg tataattata tctccattgc
    10921 tgagtgaaat cttgtaatat tctgaaagag ataataacgc agagtaaata tcataagctt
    10981 caatatcaat tgccattaaa ccaattgcag aaagagtatt gtaaagaaag tgaggtttga
    11041 tttgttcctg catagacata atttcaaagc ggtataacga attttcggaa gtccgtactt
    11101 tttcaattaa tttttttgta tcctgaacca tattaataaa tacatcattc attttcccta
    11161 tttcatcaga gcctgctata cttacagaca cctcaaaatt gcctttccca acctcagtca
    11221 ttgcttttgt taatatcata atacgttttg taattatagt aacataaaca aatgttataa
    11281 ttatactggt aacaatccct aaaatagcaa gaaatatagc aaattttctt acagcaagag
    11341 tctcgtttag aaaaaatgat tcctcaacta tattaataag tctccatccc attggattaa
    11401 tagtattata aaatatcatc atggaattac catcaatttt agtaacaaaa ctaccatttt
    11461 cctcattttt aataacagaa aaatattcat tagctgatat atttgttgta attatatcac
    11521 tgtcattgct gtatattata ttacctctat catcagtaat tacaagcttg ttatttggtg
    11581 tatcaagaaa agagaaaagt tgttttacat caatctcaat agtaattaca gctatcttct
    11641 cttttgtata tacatcaaga atttctacaa gtccgcaaat aatataaaaa tcactttcat
    11701 gcttacgcct tttaattgta ttcagccacc tatgtatatt aatcctttcc ggcctattat
    11761 gtaccatatt ataccattca gtggatttaa actcattcaa ggaaaagaac attctattat
    11821 caactttcaa agactcgttt cctgtatata tttttattga cggcaaaggt tcataattca
    11881 gtgtaagaga atctacgtaa ttatacaccc aagtctgaaa atctagctgc tcagcaatgt
    11941 ttgcgaattc ctgcacaaga gattctctaa agcttttatt aaggaaaaag gtattaagta
    12001 ttttttcata gctatttaat gtaaatacaa tattgcgatt tgtttggtta atattctgaa
    12061 gataagtttc atttttcttt tccactataa gagaataaga ccttgtaaat aagatgtatg
    12121 tggtaaaact aatgaaaagg aaaaatatga aactaaaaaa tataataaat ttatacttta
    12181 tcctgatatt attaatcaaa cccttaacaa ttcttttaac catattgtct ccttgacaaa
    12241 aataatttac tttttacgat attcacctgg gcttatacct atattgttct taaatacaga
    12301 tgcaaaatgc gttgtattat tatatccaat tctttgtgct atctcaaaaa ccattagatt
    12361 cgtatcttca agcagcttta tagcattttg cattcttacg tatgtaaggt attgtattat
    12421 tgtttctccg gtttcatttt taaacactgt acttgcatac ccgggagaaa ggttgatatc
    12481 ttctgcaatt tgctcaactg atactatact gtcgtatttt gcgctaataa tttctttaat
    12541 atgttttaca attattgcat ttttgttctt aacatttgaa tatatcttaa agctcatttc
    12601 gagtatgatc ttctttaacc aattccatat gctatcaatg ctcccgaatt tttcaagctt
    12661 ggctacaata tccgttatat ctttcaagtc agactcagct atagtaggat attcatttat
    12721 agtattaaga catgcactta gtatttctat ggaaaggttc ttaatataat gaatattaaa
    12781 gtattggctg taggaagtta taccaagtat ttcatcaagt aaattagata ttgatcttcc
    12841 gtctcctatt tttaggaatt taatgatttc cagtttataa ttttcgctat caaaccctga
    12901 tattccaatt aatttaacat cgttatagaa aaatatctga ttagctccat aataaaactt
    12961 atattgtatg gcatcaatac attttttgta ataatccgga acacttacta agttataaaa
    13021 aaatcctcct attcctattg tacaggtttg catgcagctt agttctatat tccgtctcat
    13081 aatatcgcat gttttagaaa ttacctcttc attaaagttc gaatcacatc cactctgatt
    13141 ctgtattata attacatact ctccgaattt tgaggaaaaa caatatgaaa ttccatatct
    13201 ttctatagca tcattaatac aatccaaaac cctggcattc agaagctgtt tttcatattc
    13261 gctgtaatca tacttatctt taaaatcatc aagctgcaat acaattacta tagtttctac
    13321 atttttttct gatttatttg gtactatgaa aggtatattt aaaaaatcta attttctttt
    13381 taatatatct tcatccttaa atgagccaaa tatccattct ctaaaaaagc tttcttttag
    13441 taaaggaaga ctttgttcaa gttgcaactt gaatatctcc atttcaaatt ttttcttatt
    13501 ttcctcaata cattgtttgg caacatcact gagcagatta ctcagagaaa aaatattaac
    13561 aggcttaact atataatgaa ctacattgta ttctattgct gcttttgcat aacagaattc
    13621 ctggtaccct gaaataaaaa taacttttat atccgggaac tcctccttaa gcaggttagc
    13681 aagatgtatg ccgtttttta caggcatgct tatgtctgtt aaaacaatat taggccgcgt
    13741 atttcttatt aattgtatag catcttctcc atttgctgca tatccaacaa gctctataaa
    13801 gatatcatcc cagtttatcc ctctaacaat taaatctcta tcagatactt catcatcaac
    13861 aaggaaaagc tttatcatat aaccctcaca tgtatattct aaatgcgttt atatgtatgt
    13921 aatgtttata atccataatt atattatatc ttactatata ttatttgcaa gtataactgt
    13981 atgagagtat gagaggggtg cattttctat atttgaggga aaatatggca agaagcgatt
    14041 tccatgtata
//
LOCUS       DAOSDD010000107        13965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1716_length_13965_cov_5.925306, whole genome shotgun sequence.
ACCESSION   DAOSDD010000107 DAOSDD010000000
VERSION     DAOSDD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_13965_cov_5.925306"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PK733_10575"
     CDS             complement(<1..140)
                     /locus_tag="PK733_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ11022.1"
                     /translation="MIIVSCFKHVVAGILIRNGKVLLQKPTNDTGYAFPGGHVEFGET
                     NE"
     gene            complement(412..2178)
                     /locus_tag="PK733_10580"
     CDS             complement(412..2178)
                     /locus_tag="PK733_10580"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOJ11023.1"
                     /translation="MNAIDIRKKFIEFFSNHQHSVIPGSSLIPENDPSVLFTTAGMHP
                     LVPYLLGEPHPLGKRLVNYQKCLRTDDIDEVGDDIHLTFFEMLGNWSLGDYFKDEAIR
                     MSYDFLVNWLQLDPDRLAVTVFEGDGDAPKDEEAAKVWKSLGIKEENIYFLGKKHNWW
                     GPAGQTGPCGPDTEIFYDSLKPKCSENCNPTCDCGKYWEIWNNVFMQYYKNADGTFTA
                     LQQRNVDTGLGLERMTAILQGKESIFETELFSDIIKQIKTLAKKENEKSTRIIADHIR
                     ASCFILIDGVIPGNVEQGYILRRLIRRAIRHMRKIEINPDQISELSYVVIESLRELYS
                     ELDGQQDYIINSLIEEKNKFTQTLEKGEKQFYKALEVCRKEERCTLDSKTVFTLYDTF
                     GFPPEITAELASENGLTIDYAGFEELYKQHQELSRQGAQQKFRGGLADNAEETIALHT
                     ATHLLHKALKIVSGEHVHQKGSNITKERLRFDFSHGQKMTPEEIQKVEGIVNAVIEEG
                     LQVECTEMLLEEAEKIGVEGLFTEKYGDIVKVYSIGNFSREVCGGPHASNTADLGHFK
                     IVKEESVASGVRRIKAVTTRRE"
     misc_binding    complement(2281..2511)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2559..4184)
                     /gene="gpmI"
                     /locus_tag="PK733_10585"
     CDS             complement(2559..4184)
                     /gene="gpmI"
                     /locus_tag="PK733_10585"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ11024.1"
                     /translation="MSNKKPVVLVVMDGIGLNDNEYGNAVKAAYMPTLSSLMRTCPNI
                     AIKAHGTAVGLPSDEDMGNSEVGHNALGCGQIYAQGARLVNESIENGKMYSSQTWNTI
                     IGNCIENRSVLHFIGLLSDGNVHSNIAHLKSMLKRAKEQGVSKTRIHILLDGRDVEAT
                     SALEYVDELEKLLLELNCADFDGRIASGGGRMKITMDRYEANWEMVRLGWETHVLGMG
                     RQFGSAASAIETYRKEYGVIDQDLPPFVIADDGDPVGIIYDNDSVVLFNFRGDRAIEI
                     SKAFEYENFSKFDRVRYPKVIYAGMLQYDGDLKLPRRFLVNPPDIKNTLSELLVKHGI
                     KQYAVSETQKYGHVTYFWNGNRSEKFDEILEDFEEVPSDKVPFEQRPWMKAAEITDKL
                     IDAIKSGKYTFLRANFPNGDMVGHTGNFAAARIAVEAVDLCLGRILEVVDGVGGILVV
                     TADHGNADEMYEKVKGNETPRPKTAHTLNPVPFIICDKLEQHEIAKGSFGLANAASTI
                     VELLGIEKPDMWEESLLHRSTADEECIGVKFNF"
     gene            complement(4349..5242)
                     /locus_tag="PK733_10590"
     CDS             complement(4349..5242)
                     /locus_tag="PK733_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ11025.1"
                     /translation="MNNFLVGMYGKFDDFKYKRDFRPGFWGIEACMFPDEQEVDKLVS
                     KTIKDGFNFGVHYPLVKKDTPYRDPFLIALSADEREKAWEYFENEVLFASGKGAAYIL
                     THFPKPVLVNRSLDLNYWRFAGDKEWMFTDEYPLEAMKENLAIMFKRLSDISDRYDIQ
                     IFLENDAMSAVLTESRLLVDLLKENDKIRLCLDIGRLHLQEKLDPSFNAMEFAEIMAP
                     YTGHIHLWNTNPTGNLSGGHYPVLPKQKPSEGWADISRFIHIIKGFNSKVKVLFEHRS
                     DLVNDEELDECYRWVEELLGG"
     gene            5441..5869
                     /locus_tag="PK733_10595"
     CDS             5441..5869
                     /locus_tag="PK733_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11026.1"
                     /translation="MSLCKNCGKEARVHLTDLGGALFDISKFNDVCRNVIATLSSDEI
                     YNALAEWSAQYDKEFHDLITKYRGYTIKVLSIGRGSNKPRKGYANWSDVKPAISYFYD
                     EFYSDCFVPNCWPEAVSKVDAVEIIRAYINIYDETNDNTV"
     misc_binding    6204..6447
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6497..8170
                     /gene="argS"
                     /locus_tag="PK733_10600"
     CDS             6497..8170
                     /gene="argS"
                     /locus_tag="PK733_10600"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ11027.1"
                     /translation="MEKYHKKISEVINLSFPGLDVIFTTPPDSSLGDISLPCFAFAKT
                     LRKSPNMIADELKSKLSNLEFVEKIESNDGYLNFFIKKAILFEDVITGILCMKDEYGS
                     SKSGIGKQALVEHTSINPNASPHVGRARNALIGDSIVRLLRFEGYFVDVHYFVNDIGK
                     QIAMLLLGAMDKVSVSFHDLLGIYIDINNKVNENPELEKEVFDLLYKLENGDDNIRSM
                     FRKLVNVCIDGQTSMLGELGIKYNTFDYESDYIYSQTTSKLLDEFKKTGRLEEDAEGR
                     LVLNQAEYKLPMKAPYLVLTRKDKTSLYPLRDIAYTIDKVKANADMNIIVLGEDQKLY
                     FRQVCSALDLLGYRTPSSVHYSFVLLAEGKMATRKGNVVLLEDFMREATSKASVEIEK
                     RHGHVDVNTAKAIGYGAVKYAILRASNDKNVVFDWESALSFEGDSGPYLQYSLARINS
                     IFRNYGEALPEEIDFSALQNNHEIDLVLELANFPATVKTALKELSPHVIANYVYGLTK
                     KFSVFYHYNSVLNANNENLKMARLALMAAVRQVILNCFELLGIEPVEEM"
     gene            complement(8233..8451)
                     /locus_tag="PK733_10605"
     CDS             complement(8233..8451)
                     /locus_tag="PK733_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11028.1"
                     /translation="MNIKALLLDFYGTLVYEDGEVISRVCEKIRKMSPYCPETREIAT
                     YWWEKFFSSGDNRADIECYNLLDLVGYV"
     gene            complement(8448..8666)
                     /locus_tag="PK733_10610"
     CDS             complement(8448..8666)
                     /locus_tag="PK733_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ11029.1"
                     /translation="MKVDINMADRRLKKSLDFANKQGIPYVVVIGENELESGELLLKE
                     MKIGNEYRTCMEKLEGFSMKLLSEGERA"
     gene            complement(8814..9488)
                     /locus_tag="PK733_10615"
     CDS             complement(8814..9488)
                     /locus_tag="PK733_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11030.1"
                     /translation="MIVEGGCDKKYLEEFAKYFSIKQPKIISLDGVDNAEKYLNFYDS
                     YYKNNTSIYKPKVKILFDNDAAGRDIYAKIKQKIYKSIDVSFHLIQNFDGSSNTKLTK
                     NNCNHEIEDLLYPEVMCWLVNNLLIRKGLNKVNTEEVAKKILSPAYKNDGILSLLQSE
                     KNNNNLEKGNEISFISSRKATNDIKNGLAGMFNIEGDRQLITIMDVCRSKYPFIERYL
                     KQLFDF"
     gene            complement(9531..10634)
                     /locus_tag="PK733_10620"
     CDS             complement(9531..10634)
                     /locus_tag="PK733_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ11031.1"
                     /translation="MISMTGVNIKRFRSILNLEFSIDSVYNLVAICGENNVGKTNTLR
                     AIDLFFNPDNYDITTDRPTLKQAQGGASIDPKITIEFYDSDSNFFYSVSRDFKLYEFN
                     SNNGLSGEKYHKSKTGKTKNNTTKRNLDKKELTSFIDNFEFRYIESININIPKIVEQL
                     TEDIIDVEYKGTRMTESKKALKEAYDNYTSGLQNILNVFSNSITGTFNDFRNNWNISF
                     SVSKSVDTFRDLISDDVEFYVDDKGCTSVEQKGSGLQRLAVILLNFEILKRLKRKKNI
                     IVCVDEPDIYLHEGLQRKLMQFFELQSSSMQVLYTTHSKIFINSYSMKNVILLGSKIY
                     EQYSARKKKNINVSETITIDTNDDNGYEKICTH"
     gene            complement(10634..11386)
                     /locus_tag="PK733_10625"
     CDS             complement(10634..11386)
                     /locus_tag="PK733_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOJ11032.1"
                     /translation="MVEKRIKKWEPDDFELEMNTVWSFPDRGSWATHDSKWRGNWSPY
                     IPRNLLLRYSSEGDLVLDQFAGGGTTLVEAKLLNRNIIGVDVNQDALERCREKIDFEY
                     KNAGQVYLYKGDARNLDFVSDESIDFICTHPPYANIIEYSENIDEDLSHCKVPQFLEE
                     MKSVASESYRVLKKGKFCAILMGDTRQKGHVIPMSFEIMKTFEAQGFKLKEIIIKEQH
                     NCKATGYWKTNSVKYNFLLLAHEYLFVFRKDK"
     gene            complement(11396..12259)
                     /locus_tag="PK733_10630"
     CDS             complement(11396..12259)
                     /locus_tag="PK733_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOJ11033.1"
                     /translation="MRNFDDWLSKFRLSISGYDYYVNFDKVVENVEKLKIELNILNSL
                     IGSKDIENEFEKVITKYPETLKCIPLLLAVRGNEIYAQDEDGAFTYKFSEMNYSLEQY
                     KVFMRKTGLFDLIANHLVNNLVDYALGVETGLDSNGRKNRGGHQMENLVESYIRKAGF
                     IKDETYFKEMYLADIESKWGIDLSALSNQGKARKRFDFVVKTGAMIYAIETNFYGGTG
                     GGSKLNETARSYKMLAQEADTVDGFTFVWITDGTAWRSARGNLRETFDVLDTIYSIDD
                     MEHGIMKTLFI"
     gene            complement(12264..12794)
                     /locus_tag="PK733_10635"
     CDS             complement(12264..12794)
                     /locus_tag="PK733_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11034.1"
                     /translation="MEIRRGTKKIRKNTLQAIIEFVFENYIIYVFQGALSQFDILVKY
                     KKDDSRIRTPKHIHWVVDMLMKMQGNEVLAKQFLTEIQRCWVECVPLENNNYETLKEL
                     IENGEETLNICEYAPLNGYGEYDVEFLYVLMQLLAVQEKTNRADAYMFGDIIDQLLEV
                     DRDIFKIVSTAGFNGR"
     gene            complement(12807..13706)
                     /locus_tag="PK733_10640"
     CDS             complement(12807..13706)
                     /locus_tag="PK733_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOJ11035.1"
                     /translation="MKKVQLKPFVKWAGGKGQLINVIRNTYPRDLGKEINKYAEPFVG
                     GGAVLFDILSNYELDSIYISDINPELINAYRIIRDDVEELISLLSVMQNEYVPIDTES
                     RKQYYYKKRERYNDLKINGDEAINIEKAALFIFLNKTCFNGLYRVNRKGLFNVPIGAY
                     KNPCICDTDNLRNISIALQKVTIVCGDYKESDKFIDQNTFVYFDPPYRPLTETANFTS
                     YTENLFDDKAQIELAEYVKILDKKGAKVVVSNSDPKNSNTEDEFFDELYKEHRINRVQ
                     ATRMINCNGESRGKISELLIANF"
BASE COUNT         4231 a         2924 c         2098 g         4712 t
ORIGIN      
        1 tcttcatttg tttcgccaaa ttctacatgc ccgccgggga aggcatatcc tgtgtcattg
       61 gttggctttt gcagcagaac cttgccatta cggataagaa ttccagcaac aacgtgttta
      121 aaacaactta ctatgatcat attattaaag atttaggtga cgcactaaat ctaacattag
      181 acaggcgttt cctaagagcc ggagacataa gacaaatcgt ggcagatagt aaaaaagaaa
      241 tctaaaatac acaacatatt tttgaaatag aaaaacctct gtattccttt attattatgg
      301 attacagagg tttgttttaa ttttcaccgt taaagttgag ataatggtgg tgtaaagtaa
      361 taaaagaatc tttgaaattt tccttgtaaa atagcagctg cttacattct tttattctct
      421 tcttgtagta acagctttaa ttctgcgcac tccagaagcg acactttctt ccttaacaat
      481 tttgaaatgt cccagatctg cagtattact agcgtgaggc ccaccacata cctccctgct
      541 gaaattacct atcgaataaa ccttgactat atcaccatat ttctctgtaa aaagaccttc
      601 aacaccaatt ttttctgctt cttcaagaag catttcagta cattcaacct gcagcccttc
      661 ttctattact gcattaacaa taccttcgac tttttgtatt tcttctggag tcattttctg
      721 tccatgtgag aagtcgaacc tcaatctctc cttggtaatg ttgctgcctt tttgatgaac
      781 atgctcaccc gatacaattt ttaatgcttt atgcagtaga tgagtagctg tgtgtaaagc
      841 aatcgtttct tctgcattat cagccagtcc acccctaaac ttttgctgag ctccctggcg
      901 ggataactcc tgatgctgct tatacaactc ttcgaagcca gcataatcta tagttaagcc
      961 attttctgaa gccagttcag cagttatctc cggtggaaag ccaaaagtat cgtaaagagt
     1021 aaacactgtt tttgaatcca gggtgcatct ttcttctttc cggcacactt ccaaagcttt
     1081 atagaactgc ttttcacctt tttcaagcgt ttgtgtaaac ttgtttttct cctcaataag
     1141 actgtttata atataatcct gttgcccgtc caattcacta tatagttccc gcaaagactc
     1201 tattactaca taactcaatt cagatatttg atcagggttt atttcaattt ttctcatatg
     1261 ccggatagct cttcttatta gtcttcgcag aatatacccc tgctctacgt tgcccgggat
     1321 tactccgtct atcaatatga agcatgacgc cctgatatga tccgcgatta ttctggtact
     1381 tttctcattt tctttctttg caagagtctt aatctgcttt atgatatctg aaaagagttc
     1441 tgtttcaaat atactttcct ttccttgtaa aattgcggtc attctttcaa gcccaagtcc
     1501 ggtatctaca ttcctttgtt gcaatgccgt aaaggtacca tcagcatttt tgtaatactg
     1561 cataaaaaca ttattccata tctcccagta ctttccgcaa tcacatgtgg gattgcagtt
     1621 ctcagaacac tttggcttaa gactgtcata aaaaatctca gtatcgggac cacatggtcc
     1681 ggtttgacct gctggccccc accagttatg ttttttcccc aggaagtaaa tattctcctc
     1741 cttaatacca agtgacttcc atacttttgc tgcttcttca tcttttgggg catctccatc
     1801 cccttcaaac actgttacag ctaaacgatc cggatccaac tgaagccaat tgacaaggaa
     1861 atcataactc atccggattg cctcatcttt gaaatagtca cctaacgacc agttaccaag
     1921 catttcaaag aatgtaaggt gaatatcatc tccgacctca tcaatatcat ccgtcctgag
     1981 acacttctga taatttacca gacgtttccc taacgggtga ggctcaccca gaagataggg
     2041 tacaagtgga tgcatccctg cagtagtgaa taatacactt ggatcatttt ctggtataag
     2101 ggatgatcct gggattacac tgtgctgatg gttactaaag aattcaataa acttttttct
     2161 gatgtcgata gcgttcattt ttattacctc ctcaattttt tggcaatttt gtttgaactt
     2221 cgacacagca attatacttc ccggcaggca ttaacaaata aaaaaaccct tcaatcccta
     2281 taaagggacg gaagagttat ccgtggtacc accctaatta gcttaatgct cactcaatca
     2341 gtactaacat actgtgcctt tgtaacgtaa ggtaacgtcg ttctctactc gttttacctt
     2401 tcaagaagaa gctcaggagc tgcgttcaat gtagttacct gttagatttc caccaatctc
     2461 taactctctg aaaggagttc tacacctaat cctctccgtc attgccgttg atattttctt
     2521 tatattatat atattatatc caaagtctgc aagatatatc aaaaattaaa tttaactcct
     2581 atacattcct catcagcagt tgacctatgc aacaaacttt cctcccacat atctggtttc
     2641 tctatcccaa gaagctcaac aatagtggat gctgcgtttg caagaccaaa actccccttt
     2701 gctatttcat gctgttccag cttatcacag atgataaatg gaacaggatt taatgtatgt
     2761 gcagttttag gccttggagt ttcattacct ttcacttttt catacatttc atccgcgttt
     2821 ccgtgatctg cagttacgac caatattcct ccaaccccat caacaacttc taaaatcctg
     2881 ccgagacata gatcaaccgc ttcaaccgca attcttgcag cagcaaaatt accggtatgc
     2941 cctaccatat ctccattagg aaaattggct cttaaaaatg tgtacttacc cgactttata
     3001 gcatcaatca atttatctgt gatctctgca gccttcatcc agggacgctg ttcaaaaggc
     3061 actttgtctg atggcacttc ctcaaaatct tcaagaatct catcaaactt ttcacttctg
     3121 ttgccattcc agaaatatgt aacgtgccca tatttctgag tttctgatac agcatattgc
     3181 tttataccat gcttcaccaa caactccgaa agagtatttt taatatcagg cggatttaca
     3241 agaaagcgcc tgggaagttt taaatcacca tcatactgca gcataccggc atagataact
     3301 tttggatacc tgactctatc gaatttggaa aaattctcat attcaaatgc cttgcttatc
     3361 tcaatagccc ggtcacctct gaagttgaat agaaccacac tatcattatc atatattata
     3421 cctacgggat caccatcatc agctatcaca aagggcggca agtcctgatc aattacacca
     3481 tattcttttc tgtaggtttc aatggctgat gctgcactgc caaattgcct tcccattccc
     3541 agtacatgag tttcccaacc taatctgacc atttcccagt tagcctcata cctatccatt
     3601 gtgatcttca ttcgcccgcc acctgaagct atcctgccat caaaatctgc acagtttagc
     3661 tcaagaagca gtttttcaag ctcatcaaca tattctaaag ctgaagttgc ttcgacatca
     3721 cgaccatcta gcagaatatg aattcgggtc tttgatacgc cctgctcttt tgccctcttt
     3781 aacattgatt tcagatgagc tatattggag tgcacattac catcagataa tagcccgatg
     3841 aagtgcagaa ccgacctgtt ttcaatacaa ttacctataa tagtgttcca ggtttgtgag
     3901 gaatacattt ttccattctc aatggattca ttaacaagcc tggctccctg cgcatatatc
     3961 tgtccacaac ctaaagcatt atgccctact tcactgttac ccatatcctc atccgagggt
     4021 agaccgacag ctgttccgtg tgcttttata gctatattgg gacaggttct cataaggctg
     4081 cttaatgtag gcatatatgc agcttttacc gcgtttccgt actcattatc attcaaacct
     4141 attccatcca ttactactaa aacaacaggt ttcttattac tcattaaaac tcccccaatc
     4201 atattatcca ggtttatcca ttatcatctt caaaacttta tattcattta ttcatttata
     4261 acgatctctg atcatgcgca tagtcgccag ttctatattc gtcacagctt taaccccact
     4321 tcatttagaa actacattat tacacatgct atcctccaag caactcctca acccagcgat
     4381 aacattcatc cagttcctca tcattcacca gatcagatct gtgctcaaat aacaccttaa
     4441 ccttgctgtt gaatcctttt ataatatgaa taaacctgct tatgtctgcc cagccctcag
     4501 acggcttttg ttttggcagc accggataat ggcctcctga taggttcccg gtaggatttg
     4561 tattccataa atggatatgg ccagtatatg gtgccattat ttctgcaaat tccatagcgt
     4621 taaaagacgg atcaagcttc tcctgaagat gaagtcttcc aatatcaaga cataacctga
     4681 ttttgtcatt ttccttaagc aaatcaacca ggagcctgct ttcagtcaac acagccgaca
     4741 tagcatcatt ttcaagaaaa atctgaatat catatctgtc tgagatatca cttaaccttt
     4801 tgaacattat agcaaggttt tctttcattg cttccaatgg atattcatca gtgaacatcc
     4861 attccttgtc tccggcaaat ctccaataat tcagatccag acttctgttt acaagaacag
     4921 gcttagggaa atgtgtaaga atataagcag cacctttacc agatgcaaat aatacttcat
     4981 tctcaaagta ctcccatgct ttctcccttt catctgcact caaagcaatc agaaatggat
     5041 ccctgtaggg ggtgtctttt ttaacaagag gatagtgaac accaaagtta aaaccatctt
     5101 ttatagtttt gctcactaac ttatccactt cctgctcatc gggaaacata catgcctcta
     5161 ttccccagaa gcccggtctg aagtctcttt tgtatttgaa atcatcaaac ttaccataca
     5221 tacctacaag aaaattattc atttctggat taatctccct ttatttattc tatcttttca
     5281 ttatctttta aaaacacaat aaaaacaaca gtaattttca aatatttgta tatggaagct
     5341 ttgtccggca aaataataat atgctgatta aggaaatctg cggaatgata aggtagtaat
     5401 tttttattga aaatgcattt taaatctgat ggaggctttt atgagtttgt gcaagaattg
     5461 cggaaaagaa gccagagtac acttaaccga cctaggtggt gccctatttg atattagcaa
     5521 gttcaatgat gtatgcagga atgtgattgc cacattaagc agcgatgaaa tatataatgc
     5581 acttgcagaa tggtctgcac aatatgataa ggaattccat gacctgataa ccaaatacag
     5641 gggttacacc ataaaagttc tgagtattgg cagaggttca aataaaccca gaaagggtta
     5701 tgcaaattgg tccgatgtaa aacctgctat atcttacttc tatgacgaat tttatagcga
     5761 ttgttttgtc ccaaattgtt ggcctgaagc agtcagtaaa gtagatgcag tagaaattat
     5821 ccgggcatat attaatattt atgatgaaac caatgataat acagtgtagt ttgaaaaaat
     5881 aaaaagttta tgtgaacaac tgggatatgc tagagatatg aagtctttta agaaaaaccc
     5941 ctctgctttt aaaggtcatg ttagtgatgt aacgatggtg ttgaggatcg cattgacagg
     6001 ccgtactaat acccccggat ctttataaaa tcatacagat aacggggaaa agcagggtta
     6061 tcgaacggtt gaaaagtata tatagcagta tagtataaat tgaaattctg tttcactcta
     6121 caaaatttac aaattttagt cataacatat tgacttatgc ggtaatacta tatagaataa
     6181 ggaacaataa aagtttaata atggcaaact caatgaaccg gaaaagtact atggagtcac
     6241 ttttggcagc gaatcaggga ttggtgcaag cctgatcttg atactctggg gaaaagagcc
     6301 ggggagaatt ctctttaaaa atcactgctt attgcatgtt gaatatagag aacgtaggtc
     6361 tgcgataaag ataagagtgg tttggatgca tgttgaaata tacgctttca ggcaattaga
     6421 gtggtaccac ggaaacccgt ctctttttta taaagagata gggtttttta tttaccagaa
     6481 aggagagatt aatattatgg aaaaatatca taagaaaata tctgaagtca tcaacttatc
     6541 atttcctggg ttggatgtga tctttacaac acctcctgac tccagtcttg gagacatttc
     6601 actgccatgc tttgcatttg ccaaaactct aaggaaaagt cccaatatga tcgccgatga
     6661 attaaaaagc aaactttcaa acctcgagtt tgtcgaaaaa atcgaatcaa acgatggcta
     6721 cttaaacttc tttattaaga aagctattct tttcgaggat gttataactg gtatactctg
     6781 tatgaaagat gagtatgggt cctcaaaatc cggaattggg aaacaggcat tagtcgaaca
     6841 caccagtatc aacccaaatg catcaccaca cgttggcaga gcccgaaatg cccttatagg
     6901 ggattcaatt gtaagacttc taaggttcga aggttatttc gttgacgttc actattttgt
     6961 aaatgatatt gggaagcaaa ttgctatgct gcttctcggt gcaatggata aagtaagtgt
     7021 gtcctttcat gacctgcttg gaatttatat cgacatcaac aacaaggtta acgaaaaccc
     7081 cgaacttgag aaagaagtat ttgacctcct ttataaactt gaaaacggtg atgacaatat
     7141 acgaagcatg ttccgaaagc tggtgaatgt ttgcattgat ggccagacat caatgctcgg
     7201 tgaacttggc ataaaatata atacctttga ttatgaatca gactatattt attcccaaac
     7261 caccagcaag cttctcgacg aattcaaaaa aaccggcagg cttgaagaag atgcagaagg
     7321 caggcttgtt ttaaaccagg cagaatataa gctccccatg aaagctccct acctagtatt
     7381 aacaaggaaa gacaaaacct ccctgtatcc gcttcgtgat atcgcctata ccattgataa
     7441 ggtgaaagct aatgctgaca tgaacatcat tgtactaggg gaagatcaga agctgtattt
     7501 tcggcaggtt tgttcagcac ttgatctatt gggctatagg acaccttcat cggtacacta
     7561 ttcatttgtt ctgctcgctg aaggaaaaat ggctacacga aaaggaaacg tcgttttatt
     7621 ggaagatttc atgagagaag ctacttcaaa ggcttctgtg gaaattgaaa aaaggcatgg
     7681 ccatgtagat gttaatacag ctaaagccat tggatacggt gcagtaaagt atgctatttt
     7741 acgtgcgtcc aatgacaaaa atgtggtgtt cgattgggaa tctgccctga gttttgaagg
     7801 tgactcaggt ccatatcttc agtatagcct ggcaaggata aattcaatct ttagaaatta
     7861 tggggaagca ttgcctgagg aaattgattt ttcagcactt caaaataatc atgaaataga
     7921 tttagtgctg gagctggcta actttccggc tacagttaaa acagccttga aggagttaag
     7981 tccacatgta atagctaact atgtatatgg ccttacgaaa aaattctcag tattttacca
     8041 ttacaattct gtcttgaatg ctaacaacga gaatttgaaa atggcacgac ttgctcttat
     8101 ggcagcagtg cgccaggtca tcctgaactg ctttgaactt cttggtattg aacccgttga
     8161 ggaaatgtaa atccaaattt aaaaccgctc cgaagttcat aacagctcct gactttcgga
     8221 gcggtgaatt tgtcagacat atccgaccaa gtctaaaaga ttataacatt caatatccgc
     8281 cctattatca cctgatgaaa agaatttttc ccaccaataa gttgctattt ccctggtttc
     8341 cggacaatac ggagacatct ttcttatctt ttcacatact ctgctgatta cttcgccatc
     8401 ttcatatacc aaagtaccat agaagtctaa taataaagct tttatgttca tgctctttct
     8461 ccctcagata gtaatttcat agaaaacccc tccagtttct ccatacaagt tctatactca
     8521 ttgccgattt tcatttcttt taacagcaat tcccctgatt ccagttcatt ttcaccaatt
     8581 acaactacat aaggaattcc ctgcttatta gcaaagtcca atgatttctt cagccttctg
     8641 tctgccatgt ttatgtcgac tttcagtcct ttattcccac tcgctcacta aaaacgtttt
     8701 ctaaacaatt tcgtttatgt tttgtaagct gtattactct atttatatca attatccaaa
     8761 tattttaggc tatctagttt tctgttgcca tattgttgcc gtaattcact attctaaaaa
     8821 tcaaacaatt gtttcaaata tcgttctata aacggatact tacttctaca gacatccata
     8881 attgttatta gctgtcgatc tccctctatg ttaaacatcc ctgctaaccc atttttaata
     8941 tcatttgttg cttttcgaga tgatataaaa gaaatttcat ttcctttttc taaattatta
     9001 ttgttttttt cactttgcaa caaagacaat attccatcat ttttatatgc tggtgatagt
     9061 attttttttg caacctcttc cgtattaact ttatttaggc cctttcttat taataaatta
     9121 tttactaacc aacacattac ttctggatat agtaaatctt caatttcatg attgcagtta
     9181 ttttttgtaa gttttgtatt actactacca tcaaaatttt gtattaaatg aaaagatacg
     9241 tcaatactct tatatatttt ttgttttatt ttggcataaa tatctcttcc agcagcatca
     9301 ttatcaaaaa gaattttaac ttttggtttg tatatagatg tattgttttt gtaatatgaa
     9361 tcataaaaat ttaaatattt ttctgcatta tccacgccat ctaatgatat tattttaggt
     9421 tgctttatcg aaaagtactt ggcaaattct tccaaatatt ttttatcaca acctccttca
     9481 acaatcaaat tatatctgtc taaaatatca taattatttt tttctattcc ttaatgagtg
     9541 catatttttt catatccatt atcatcatta gtatcaattg tgattgtttc agaaacatta
     9601 atattcttct tttttcgtgc agagtattgt tcataaattt tacttcctaa aagaatgaca
     9661 ttcttcatag aatatgaatt tataaaaatc ttcgaatgtg ttgtataaag cacttgcatc
     9721 gaagaagatt gtaattcaaa aaactgcatt aattttcttt gcaatccttc atgtagataa
     9781 atatctggtt catctacaca aacaatgata tttttctttc tttttaacct cttaagaatt
     9841 tcgaaattta ataagattac agctaatcgt tgcaagcccg aacctttttg ctcaacgctt
     9901 gtgcaacctt tatcatcaac ataaaattca acatcatcgg aaattaaatc tctaaaagta
     9961 tccacagatt tcgacacgct aaacgatata ttccaattat tacggaaatc attaaatgta
    10021 cctgtaatac tgtttgaaaa tacatttaat atattttgta atcctgaagt ataattgtca
    10081 tatgcttcct ttagtgcttt tttggattcg gtcatacgtg ttcctttata ttcaacatca
    10141 attatatctt ctgttagttg ctcaacaatt ttaggaatat taatatttat tgactcaatg
    10201 tatctaaatt cgaaattatc tataaaactc gttaattctt ttttatctaa atttctcttt
    10261 gttgtattat ttttcgtttt tccagttttg cttttatgat atttttcacc agataatcca
    10321 ttattgctat taaactcata aagtttaaaa tctcgactaa ctgaataaaa gaagttgcta
    10381 tcagaatcat agaattcaat tgtaatttta ggatcgatac ttgctccgcc ttgtgcttgc
    10441 tttaaagttg gacgatcagt tgtaatatca taattatctg ggttaaaaaa taaatctata
    10501 gctcgaagtg tgttcgtttt accaacatta ttttccccac atattgctac caaattatat
    10561 acgctatcta tactaaattc taaatttaat attgaacgaa atctttttat attaacacca
    10621 gtcattgaaa tcattactta tccttcctaa acacaaacaa atactcgtgc gcaagcaaca
    10681 aaaagttgta cttcacacta tttgttttcc aatacccagt tgccttgcag ttgtgttgtt
    10741 ctttaattat tatttctttc agcttaaacc cttgtgcttc aaaggttttc ataatctcaa
    10801 aactcatagg aataacatga cctttttgcc ttgtatcacc cattaaaatc gcacaaaact
    10861 tgcccttttt aagaacacgg taactttccg aagcaacact tttcatttct tctaaaaact
    10921 gtggaacctt acagtgagat aaatcctcgt ctatattctc actatactca ataatatttg
    10981 cataaggcgg gtgagtacaa ataaagtcaa tactttcatc agaaacaaag tcaaggttac
    11041 gtgcgtcgcc tttatataaa taaacttgtc ctgcattttt atattcaaaa tctatttttt
    11101 cgcggcaacg ttcaagtgca tcctgattta catcaactcc gatgatattg cggtttaata
    11161 gttttgcttc gacaagcgta gttccgccac cagcaaattg atcaagcact aaatcgccct
    11221 cactcgaata acgcagaagc aaatttcttg gtatgtacgg cgaccaatta cccctccatt
    11281 ttgaatcatg tgttgcccaa cttcctctat cagggaaact ccaaaccgta ttcatttcga
    11341 gctcaaaatc atccggctcc cattttttta tcctcttttc taccacatcg cacctctaaa
    11401 taaacaatgt cttcatgata ccatgttcca tatcatctat gctataaata gtatccagta
    11461 catcaaaagt ttctcttaag tttcctcttg cacttctcca agccgtacca tctgtaatcc
    11521 aaacgaaggt aaatccatca actgtgtccg cttcttgtgc taacatttta tagctacgag
    11581 cagtttcgtt aagctttgag cctccgccag ttccaccata aaaattagtt tcaatggcat
    11641 atatcatagc accggttttc actacaaaat cgaaacgctt ccgtgccttg ccttgattag
    11701 agagagcaga caaatctata ccccatttgc tttctatatc agccaagtac atttctttaa
    11761 aatatgtttc gtctttgata aaacctgctt ttctaatata gctttcaacc aaattttcca
    11821 tttggtgtcc accacgattc tttcttccat ttgagtctaa acctgtttct acacccaaag
    11881 cataatcaac aagattgttc actaagtgat tggcaatcaa atcaaacaac cctgtttttc
    11941 gcataaatac tttgtattgc tccaaagaat aattcatttc actaaacttg tatgtaaatg
    12001 caccatcttc atcttgagca tatatttcat taccgcgtac agcaagtaac aaaggtatac
    12061 attttaaggt ttcaggatat ttcgtaataa ccttttcaaa ttcattttca atatccttag
    12121 aacctattag tgaatttaga atattaagct caatttttaa tttctctaca ttttcaacta
    12181 ctttgtcaaa attaacataa tagtcgtacc cagagatact taacctgaat ttgcttaacc
    12241 aatcatcaaa attccgcata ttattatctc ccattaaatc cagctgttga aactatttta
    12301 aaaatatctc tatctacttc aagcaactgg tcaattatat ctccaaacat atatgcatct
    12361 gctctgttag ttttttcttg aacagctaag agctgcatta acacatataa gaactcaaca
    12421 tcatattctc cgtatccatt cagaggagca tattcacaaa tatttaaagt ttcttcacca
    12481 ttttcaatca gctcttttag tgtttcatag ttgttattct ccaatggaac acactcgacc
    12541 caacatcttt gtatttccgt taaaaactgt tttgctaaca cttcatttcc ttgcattttc
    12601 attaacatat ctactaccca atgtatatgc tttggagttc taatacggct gtcatccttt
    12661 ttatatttta caaggatatc aaattgtgaa agtgccccct gaaaaacata tattatgtaa
    12721 ttctcgaaga caaattcaat gatagcctgt aacgtatttt ttcgtatttt tttagttcca
    12781 cgccttattt ccatactgta tatccctcaa aagtttgcta tcagtaattc gctaattttc
    12841 cctcttgatt ctccattaca gttaatcatt cgagtagctt gtacacggtt aatcctgtgc
    12901 tctttatata actcatcgaa aaattcatct tctgtattag agttttttgg atctgaatta
    12961 cttacaacta cctttgctcc ttttttatct agtattttta catactctgc aagttcaatc
    13021 tgtgccttat catcaaataa gttctctgtg taggatgtaa aattagctgt ttcagtaagt
    13081 gggcgatagg gtggatcaaa atatacgaaa gtgttttgat caataaactt atcggattct
    13141 ttgtaatcgc cacacactat ggttactttt tgcaaagcta tagaaatatt tctcaaatta
    13201 tcagtatcac atatacaggg atttttataa gcacctattg gaacattgaa taatccctta
    13261 cgattaaccc tatataaacc attaaaacag gttttattca aaaaaataaa caatgcggct
    13321 ttttcaatat ttatagcttc atcaccatta atctttaaat cattatatcg ttctctcttt
    13381 ttataataat attgcttgcg actttcagta tcaataggta catattcatt ttgcataaca
    13441 gatagtaaac taataagctc ttcaacatca tcacgaataa ttcggtatgc atttataagc
    13501 tctgggttaa tatcacttat ataaatggaa tccagttcat aatttgaaag aatatcaaat
    13561 aatactgctc caccgccaac aaagggttca gcgtatttat ttatctcctt tcccaaatct
    13621 ctaggatatg tatttctaat aacgttaatt aattgtccct tgccgccagc ccatttaaca
    13681 aaaggcttta actgtacttt tttcatatat ctcaaactcc taccatcaac aggtttctca
    13741 gcatcttttg gaataatcca catattgccc aacatatacg cttccggaat tctaccctca
    13801 gaacatagta ctgctacgcg tctttgtgag atattccatt tctctgctgc ttcttttgca
    13861 gatatgtact gcataatgga cctccttgca tactacatat tgacattata ttctatatct
    13921 cgaacaatag caacaccttg tttgcatatg ttactaacaa ggggc
//
LOCUS       DAOSDD010000108        13752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1742_length_13752_cov_7.491348, whole genome shotgun sequence.
ACCESSION   DAOSDD010000108 DAOSDD010000000
VERSION     DAOSDD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_13752_cov_7.491348"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6150)
                     /locus_tag="PK733_10645"
     CDS             complement(<1..>6150)
                     /locus_tag="PK733_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ11036.1"
                     /translation="KLDIPIASSEIKDIAADYDNHLYILKKDGTIAAFDFNPDSREYG
                     EAMEVPVRITNVKELYAGYGYCMVIKNDGTVDGWGDIELSEFSEEQFGEVVSISMDKT
                     SEDGGFSVLRRDGTANSYRRRYSYVVTVRYTDLLAICGGSESQVIVKKDGSVSCYENG
                     NLVSDIPQINIFKHKLISELVQKDEEIYVEIHINDISGSPIKSAGIYGSPGFINPVDA
                     DDFVVDIVKGSQSLIDVCIDSVKHQLQGSDGEGMGGGLWARLYLKIYGTLTGGEQIVI
                     RPKNDFSIYGNAHKNLISIQSTEPMFLNEAEAKTHLKYERWLDAGWAHGIMMKETGYG
                     FHWGDNYFGQTPFPIPVSDIYQVSAGYYFTNVVLKDGTIAGWGDNSFGQLDVPEDTGK
                     IHQISSGGYHVLVLNTNDQVIAWGNNQYNQCDVPEGLDSVIRITAGGNHSLALKKDGT
                     VTAWGDNYFGQCDVPEGLKDVIAIAAGDKHSLALKNDGTVVGWGDNSSSQISIPEGLK
                     DVLLIKAGYTHSLAIKRDGTLVAWGDNGKGQCDIPEDLENVAAVAGGRDFTIVMEKDG
                     TLHAWGDNTKKQCEFPEAANPPILLDSSIDPKNRYIDLVFNKGIYGDRLGTTPVMLDN
                     FVIEYMTNSDIEDTGNTDDAVTATVTSLKSMDSEVEGEASGLIGGDSRIRMFLQVEGK
                     PEGTETIEIKVNKDKMIYDIFGIPVNENMSDKINIIRPYDDIIRETGYFSGSSTTKIQ
                     RLEYYDNFGNKISEWHEVWGYYSDDSNTIEWKHIIENVGRNDRLLIVTLSSFEPNHSN
                     QVIEWVSLQSYPGATQPVIPLKHIATYYEGDYRNDIYYLLDNDLPYFDGEYFIKAKYR
                     GPNRAISSKSYSYINVAQMPPENVEIFDSELIPHSNKKRTTSVTTLTDRALTIEQGYF
                     YTKSSNGKEKDLGGISQIILRGDAGTYITEKNVYEGAYRGYAVTFRNVTHGTELPPNP
                     SPLPDPIELPNFPEANNIAFEKQPFAGTPIQFGDKIVVYGYGGLYISDDGEEWAGPYE
                     YPGADSFISRPDVYVCDSKLLTFVFNSDKVIVNTSFDGINWTSTECKMPVVSDEGGAY
                     IYFDDGVIVDFDWSTDSVTGQISMVGVGNVWIAQLGPDGYPKTRQGENGWVYDLMTPI
                     NIWISSQDNGNTWTARYIPEFFFQYTPESRGQYGARLFLKVLSNTNGIYIAFDNRKVY
                     FSSDKGISWIEALSFDDPDDPYAYTRYFTDIFVHNNMFITTASRLNDKLNIYWSTDGV
                     EWTVGEIGYVDPKFETYNPITREYYYNYSIDFHNMAYANNTYATVNGNTIAESVDGKT
                     WNLYYCDFPSYKLESYNNRFYRMIAYKTEKGTEYQLLGSDNLRDWYFESTTGTVGSIY
                     KDDSLSISGKVIVGSRSLLNNSVVIGYSPLPIVFTAPDDKDTHVPVNSILMVKFSEGI
                     KEGGGWADISLTDSGGMDVPVTVSMDGDNELVKIIPQSTLQHEEEYDLLIPEGFVKDK
                     NGSKTNKRKIVSFKTGVSEVPVVMGTNIREEGLPVESSIELYYSREIKIDSQGQYDKI
                     SLVEKNNPEKSIPIYKDIDGNKLIITPALPLMYEIEYIITIPEGSVEDNYEISTPAWS
                     LEFKTSADAQNPLVYETNIPVHGIPFQADRSIEITFNEEVIQSGNFENIVLKNIRSVE
                     NTSIRLELENIIYDGFQRTKLKIWPLNELTFFGEYTLIIPSNAVKDISGNWLEDEQIW
                     KFIVENKAGYPILIGLKPMNLSNLVKLSDDIIFYFDRPIKATEDFNESVFFEYIETDE
                     YGYEIARHDIEFQVVIKDNKIIITPDRNPSSGPYYYKLDSRYEVRVKSEAISNIYGNT
                     QKGEVYTIEFITERMNILTTSPENGQTNVPLNGLITVEIDDWDAYEVYYGSQFYNISL
                     QKVEDRNGTKIYTKVPAGLYLGERTIYIAPENDLEPHTEYMVDLPRNAIRMVTDITNI
                     HYTFTFITGTEMVETGTIDIMGEIMIGDSVVEGAKVTFTPNHLPVAKEDIVSYVWSYG
                     DNPYHYEYGSVGVHTYTDYGIFRVFMIATDKEG"
     assembly_gap    6151..6250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6251..>9580)
                     /locus_tag="PK733_10650"
     CDS             complement(<6251..>9580)
                     /locus_tag="PK733_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11037.1"
                     /translation="YIDITFNTGVYGDSHHMNPVNREDFTLINETNGGNATIVDLADV
                     RKPDAAEIEQASFLTGGEKTVRVFLYIPKLATGEETIEIKARGDSTIFSYSGLCMHET
                     ETTGIINLNHIPRITKTEMAIDNTYVDVHFSTGIHGSPDGLSPIEKGDFNLSLNLDTE
                     GCGLTQAEIVSVKKPDNTSEGSATPLTGGETVVRFFLALSGNETKNDNIIIGPRDGSS
                     LYDQRGTAMQIDKTTGVIYFNQRELNMLSAELAEDNSYIDVYFSTGVYGDTNGKTTVN
                     LNNFYLSGDSTIVQFSSIKKPDSTNENEASPLKGGETAVRFFLKILQHPGDDKVHILA
                     SKYDHPIYNSWGSIIKNASTDDLTLHDMRIKIVSAEMESYNRYLDIIFNKGIYNSDGS
                     PVTADNFFITLAAGGTLEKIKIISLKKPDSKIEADATPLEGGENSVRMFLELQGSPSG
                     EETFSIRVSHVEDETGVLAHYAIVVEDIHVLPPTPEIVSSEFVTEPGLYVDITFNTPV
                     FYYVLDTWGPLTKAAFKINAINLMGSSSHMEIIGVRNPDGATPEEASPLVGGAKKVRV
                     FLKQTGLSNGDDKFQISPYDSRFIVSSDKKTILTLGTQFFHPEPNLPVFTGIYSPGGM
                     NYLDISFNYGVYSYDWSWDGPIQKECFAISFKDPDKSLQVIRKSFRKPNHEIELEASP
                     LVGGEKTIRAFFEVTGEYTGDETISFQYFNEHPVYSYIQGEMRYMLLYQSSGEFKIPA
                     PGLKIEHVSKVIPVDSFGQYVDVSFSGEVYGDSQKLSSVHSDAFILELKNNEGEVTTI
                     KPRYILTVNDENLTGGEKFVRIYFNNTDENNVTMTIRAEENSIFNITGIPMKATEAVS
                     DIRLAKVHPRLTDASMGTGSKYIDIYFDRPVRSIDQDALSLDDFEFVFNQNDGNVKNP
                     KFYHISDTDGEEWHEASRPSIYSTAFRIFMEYDGTPTGAETVEIKGKQGTITDSKGNV
                     VPIDISTDPVHFVTPYLSTMEDSYTEGWMNGLVSRSSEHTLVVDTNGDVTAFGSNVYG
                     ECDVPEDLEHVKTVAAGDYFSVALKKNGQLSGWGRIKVPENVWGVKNISHSKYGVFAL
                     RYDGTIAFIGNHEFRFDPETQE"
     assembly_gap    9581..9680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9681..12962)
                     /locus_tag="PK733_10655"
     CDS             complement(<9681..12962)
                     /locus_tag="PK733_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11038.1"
                     /translation="MRKLEKLLAIVLITAFTVTQLPLAALASSAVHAASNVIGQLNLS
                     SGFSDEDILNMNTNTNNDTNMDIDDSDDTDDSNDLDDSDSGSDSKGETGTDNGTASDK
                     GDSSTEGSEDTIGNKSNNKSDDKASDEAGFTAAFATTDISGLILSHSGYMKKISSGPD
                     YTLAITSDGRVAAWGDNTQGQCNVPYDLVDVIEVSAGAYHALALRADGIVVAWGDNTY
                     GQSDVPAGLSGVIAVAAGGSHSLALKADGTVVGWGGYTPDDNSYNHDVPENIQNPESA
                     NVVSIKAGGFHSLALKADGTVVAWGNNSWNQLQIPYELTDPGTANVAAIAAPWGHNLA
                     IRKDGTVIAWGYDVFGQISVPEYLDNVVAADGSYFDSIVLKADGTIIAWGYHSGALAP
                     PPEAAGAMAIACQQTGAAALKPDNTLTSWGIADTPNGTSLIARPRIIHSYLDSTNQYI
                     DIYFSTGIYGTGMTPVSNDAFELVFENNGGDAAGAAITSVTKQDGTPLLGGESVVRVS
                     LSITGTPSGEETIEVNIPEEYEIFDSEGTYMTDRRTTGPVRLKPASLKLISTYIAPDN
                     SYIDVEFNMGVYGDLSHSVPVEIEDFDLTFSQNTGTATGIRATSIINPETEEALTGGE
                     IIIRFILEIEGNPNGEETIVIGSSSDEAVYSREGIALLQGSTTGIIQLNPPEIRFIGI
                     HLAEDNSYLDIYFNTDVYGDMDFIGKKRIGIGPVTSMNIRLVYEQNEGNIEDVTILSL
                     KKPDSTIESEATDLEGGERVIRMFLDLSEGQPNGTETVAIQVEGVFSKSGQPYFTMEP
                     EHTTNLNVLPLFTSSTVGPNNTYVDVNFNMGVYGAADGSTPVDKSDFQLNFYKNNGHA
                     ENITIISVKKADSEMENSASPLSGGETHIRIFLALSGPAAGSPDGGGTNEAIEIYPAD
                     SQSIFSQSGKSMTDITTTGMIKLKPNFPRILSAQLSNDNSYIDISFDYGVYGDINHTS
                     PITREHLHLGFYSNGGNAYNASIHKVTKCNSAVSEEASPLEGGETEARVFLDITGTPS
                     GTEECFIGSTIDNPIYNFVGNPINYFTERTGNIKFDGIPAIIKVTPAADLSYIDMELS
                     TPVYGD"
     gene            complement(13237..>13752)
                     /locus_tag="PK733_10660"
     CDS             complement(13237..>13752)
                     /locus_tag="PK733_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOJ11039.1"
                     /translation="TTLHYVKKALLLSGIEISIQYDHGSYRINMDNLYCDYLQFCTFA
                     ENTGIIGRDNILECEEALALYAGEYLSGWDYEWVAGKRLLLEEKFIELLLKMTEYYKR
                     IKNYSKVEKWLKEGLFYEPLHRELNYRLIEVLLHAHERALAAKHYNLYCTGLYKKFGL
                     EPDAAFKKLIR"
BASE COUNT         3393 a         3063 c         2650 g         4446 t
ORIGIN      
        1 taccctcctt atccgttgcg atcataaata cccggaaaat cccatagtcc gtataggtgt
       61 gcacaccgac ggagccgtat tcataatgat acggattatc tccataggac cagacatatg
      121 acactatatc ctcttttgca acaggtaagt gattcggggt gaatgtcacc tttgctcctt
      181 cgacaactga atcccctatc atgatttctc ccattatatc aatggtgcct gtttcaacca
      241 tttcagttcc agtgataaag gtaaatgtgt aatggatatt tgtaatatct gttaccattc
      301 taatagcatt tctcggtaag tccaccatat actcggtatg gggctcaagg tcattctccg
      361 gagcaatata tattgttctt tctccaagat acaaccctgc aggaactttg gtatagattt
      421 ttgttccgtt tctatcttcc accttttgaa ggcttatatt atagaattgt gagccgtaat
      481 atacctcata ggcatcccaa tcatcaattt caacggtgat gaggccattg agaggcacat
      541 ttgtttgccc gttttccgga gaggtggtta aaatattcat cctttcggtt ataaattcta
      601 tggtgtatac ctcacccttt tgtgtattcc cgtaaatatt cgagatagct tcgcttttga
      661 ctctgacctc gtatcttgaa tccaatttat agtaataagg tccgctactg gggttcctgt
      721 ccggtgttat gataatttta ttatccttaa tgaccacctg gaattctata tcatggcgag
      781 ctatttcata accatactca tcggtttcaa tatattcaaa gaacacactt tcattaaaat
      841 cctccgtggc ttttataggc ctgtcgaaat agaatataat gtcatcgctc aattttacca
      901 ggttggataa attcatcggt ttcagaccga tcagaatggg atatcctgct ttattttcta
      961 cgatgaactt ccatatctgt tcgtcttcca gccaattacc tgaaatatct ttcacggcat
     1021 tagaaggtat tatcagagta tactccccaa agaaggtcag ttcatttaaa ggccatatct
     1081 tgagctttgt tctttgaaaa ccgtcatata ttatgttttc aagctctaag cgtatactcg
     1141 tattctcaac ggatcgtatg ttctttaaaa cgatattttc aaaattgccg ctttgtataa
     1201 cctcttcgtt gaaggtgatt tctatgctcc tgtctgcctg gaagggtatc ccgtggacag
     1261 gaatattggt ttcatataca agtgggtttt gagcatccgc ggaagtttta aactccaaag
     1321 accaggctgg ggtactgatt tcataattat cttcaacact tccttcgggt atggttatga
     1381 tatattctat ttcatacatt aatgggagtg caggagttat gatcagttta tttccgtcga
     1441 tatccttgta tatagggatg gacttttcag ggttattctt ttccacaaga gatatcttgt
     1501 cgtattgccc ttgggaatct atttttattt cccttgagta atagagttct atggaggatt
     1561 ctacaggcag accttcttct cttatgtttg tgcccatgac cactggcact tccgacaccc
     1621 cggttttaaa gctaacgatc tttcgtttgt ttgttttgga gccattctta tcctttacga
     1681 acccttcagg aattaagagg tcatactctt cctcatgttg aagggtgctt tgcggtatga
     1741 ttttcaccaa ctcgttgtcc ccgtccatcg atactgtgac aggcacatcc atccccccgg
     1801 aatctgtaag ggatatgtct gcccaacctc ccccttcctt tattccttcg ctgaacttaa
     1861 ccatgaggat gctgtttacg ggcacatggg tatccttgtc gtccggtgct gtaaatacta
     1921 tggggagagg tgaataccct attactacac tattgtttaa taagcttctc gacccaacaa
     1981 taaccttgcc actgatagaa agactgtcat ctttatagat ggatcctaca gtgcctgtgg
     2041 tgctctcaaa gtaccagtcc ctgagattgt cactgcctag gagctggtat tccgtgcctt
     2101 tttccgtttt ataggcaatc atcctataga agcgattgtt atagctttca agcttgtagc
     2161 ttggaaagtc acagtagtag agattccatg tctttccatc gacagattcc gcaatggtgt
     2221 ttccattcac ggttgcatag gtattatttg cataagccat gttatggaaa tctatggagt
     2281 agttgtaata gtattctctt gtgatgggat tgtaggtttc aaatttcgga tctacatatc
     2341 ctatttcccc aacagtccac tctaccccgt ccgttgacca gtatatattc agtttatcat
     2401 tcagcctgga ggctgttgtg atgaacatat tgttatggac aaatatatcg gtaaaatagc
     2461 gggtatatgc ataaggatca tccgggtcgt caaaactcag ggcttcaatc cagcttattc
     2521 ccttgtctga agaaaaatat accttccggt tatcaaatgc aatatatatt ccgttggtgt
     2581 tggagagcac cttcaggaac aacctggcac catattgccc gcggctctcc ggggtgtatt
     2641 ggaaaaagaa ttccggtata taccgggcag tccaggtatt gccgttatct tgtgacgaaa
     2701 tccatatgtt aatgggggtc attaggtcat atacccatcc gttttctcct tgtcttgttt
     2761 tcgggtagcc gtccggacca agttgagcaa tccagacatt acccacacca accattgata
     2821 tttgacctgt tacagaatcc gtagaccaat caaagtctac tatcactcca tcgtcaaaat
     2881 agatgtaggc tcctccctca tcacttacaa caggcatctt acattctgta ctcgtccagt
     2941 ttataccatc gaaagaggtg ttgacgatta ccttgtcgct attgaacaca aaggttagaa
     3001 gcttgctatc acatacatac acatccggtc tgctaataaa tgagtctgct cccggatatt
     3061 cgtacggtcc cgcccattct tcaccgtcat cagatatata aagaccacca tatccataga
     3121 ccacgatttt gtctccaaat tggatgggtg taccggcaaa gggctgcttt tcaaaagcaa
     3181 tattgttggc ttccggaaaa ttcggaagct ctatggggtc aggaagtgga gaaggattag
     3241 ggggaagctc agttccatgg gttacgtttc tgaatgtaac agcataacct ctataagcgc
     3301 cttcatacac attcttctct gttatgtatg tgcctgcatc tccccttagg attatttgac
     3361 tgattcctcc taaatctttt tccttcccgt ttgaggattt tgtataaaaa taaccttgtt
     3421 cgatagttaa cgcacgatca gtcaacgttg tgaccgatgt ggtccttttt ttgtttgagt
     3481 gtggtatcag ctccgaatca aatatttcaa cattctccgg aggcatttga gctacattga
     3541 tataactgta ggatttactg gatattgctc tgtttgggcc tctgtatttt gcttttataa
     3601 agtattcacc atcgaagtaa ggaagatcat tatcaagtaa atagtatata tcatttcgat
     3661 aatcaccttc ataataggtt gcgatatgtt tcaatggtat gacaggttgt gttgcaccgg
     3721 ggtagctctg caatgacacc cattctatca cttgatttga atgatttggt tcaaagctgc
     3781 ttaaggttac aatcagtaat ctgtcattac gtcctacatt ttctattata tgcttccatt
     3841 caattgtgtt tgaatcatcg gaataatacc cccatacttc gtgccattcg ctgattttat
     3901 tcccgaaatt atcataatat tctaaacgtt gaatcttagt cgtgctgctg ccggaaaagt
     3961 agccggtttc ccttataata tcgtcgtaag gccttattat attaattttg tcgctcatat
     4021 tctcgttaac aggaatacca aatatgtcat agatcatctt gtccttgttc acttttattt
     4081 ctatagtttc cgtcccctca ggcttgcctt ctacctggag gaacattctt attctgctat
     4141 ctcctcctat aagcccggag gcttcgcctt caacctcact atccatagat ttcagagagg
     4201 ttaccgttgc tgtaactgca tcatcggtat tcccggtatc ctcgatatcg gaattagtca
     4261 tatattcaat gacaaagttg tccaacatta cgggggttgt tcctaggcga tccccatata
     4321 ttcccttgtt aaataccagg tcaatatagc gattcttggg atctatgctg ctgtccaaca
     4381 atatgggggg attagcggct tcgggaaatt cacattgttt cttggtattg tcgccccaag
     4441 catggagagt tccgtctttt tccatgacta tggtaaagtc tcttcctcct gccactgccg
     4501 ccacattttc aaggtcttca ggaatatcac attgcccttt tccgttatct ccccatgcta
     4561 ccagagttcc gtcccttttt atagcaaggg agtgagtata gcctgctttt atgagaagaa
     4621 catccttaag gccttcaggt atgcttatct ggcttgagga attatctccc cagcctacaa
     4681 cagttccatc attttttaat gcaagagaat gtttgtctcc tgcagctatg gctatgacat
     4741 cctttaatcc ttccggtaca tcgcattgcc cgaagtagtt gtcaccccag gctgtcaccg
     4801 tcccatcctt ctttaaagca agggaatggt ttcctcctgc agttatacga ataacagaat
     4861 ccaagccttc cggtacgtcg cattgattat attgattgtt tccccaggca attacctggt
     4921 catttgtgtt aaggacaagg acatgatagc ctcctgagga aatttgatgg atttttcctg
     4981 tgtcttctgg tacatccaac tgtccgaagc tattatctcc ccatccagcg atagttccgt
     5041 ccttcaagac tacattagtg aaataatatc ctgcagatac ctggtatatg tcggatacag
     5101 gtatgggaaa gggtgtctgc ccaaagtagt tatctcccca gtggaagcca tatcccgttt
     5161 ctttcatcat gattccgtgg gcccagccgg cgtcaagcca cctctcatac ttaagatggg
     5221 ttttcgcttc tgcttcattt aaaaacatgg gttctgtgga ttggatgctt attaaattct
     5281 tatgagcatt cccgtaaatg ctaaaatcat ttttcggcct gattacaatt tgctcacctc
     5341 ccgtgagggt tccatatatc ttaaggtaaa gccttgccca aaggcctcct cccatacctt
     5401 caccatcact tccttgcagt tgatgtttta ctgaatctat gcatacatct attaaacttt
     5461 gacttccttt aacaatatca acaacaaagt cgtcggcgtc aaccggattt atgaatcccg
     5521 gagagccgta tataccggca ctttttatag gacttcccga gatgtcgttt atatggatct
     5581 ctacatatat ttcttcatct ttttggacca actcactaat caatttatgc ttgaaaatgt
     5641 tgatctgggg aatatctgac acaaggtttc cattttcata gcatgataca ctgccatctt
     5701 tctttacaat aacctgactt tcagatccgc cacatatagc cagtaagtcc gtatatctaa
     5761 ctgtaacaac atagctgtat cttctcctgt agctgttagc agttccatcc cttcttagta
     5821 cactgaaacc tccatcttca gaagttttgt ccatggatat tgatactacc tctccaaatt
     5881 gttcttcaga aaattcagag agttcaatat caccccatcc gtcaactgtg ccgtcgtttt
     5941 ttataaccat acagtatcca tatcctgcgt agagctcttt tacgttggtg attcgtacgg
     6001 gcacttccat ggcttctccg tattcccggc tgtctggatt gaaatcgaag gccgctattg
     6061 tgccatcttt ttttaggatg tacaggtgat tatcataatc tgcggctata tccttaattt
     6121 ccgaagaagc tatgggtata tccagttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn ttcttgggtt tcaggatcaa atcgaaattc gtggtttcca ataaaggcaa
     6301 tggttccgtc atatcttagt gcgaagacac catatttact atgagaaata ttctttactc
     6361 cccatacatt ttccggcacc tttattctgc cccatcctga taactgtcca ttctttttca
     6421 aggctacaga gaaatagtct ccggcagcta cagttttaac atgttcaaga tcctccggca
     6481 catcacactc tccgtacaca ttagagccaa atgctgttac atctccgttg gtgtccacaa
     6541 ccagggtatg ctccgaagac cggctgacaa ggccgttcat ccagccttcg gtataggagt
     6601 cctccattgt ggaaagatat ggagttacaa aatgaaccgg gtctgtggaa atatcaatcg
     6661 gaacaacatt accttttgag tctgtaatag tcccttgctt tcctttgatc tcaacggttt
     6721 ctgccccggt tggcgtccca tcatactcca taaatatcct gaaagccgta ctgtatatgc
     6781 ttggccgtga agcttcatgc cattcttccc cgtccgtgtc gcttatatga tagaattttg
     6841 gattctttac atttccatca ttttgattaa aaacaaactc aaaatcatct agtgacaaag
     6901 catcctggtc aatcgatcga accggcctgt caaagtaaat gtcaatatat ttgcttcccg
     6961 ttcccataga agcgtctgtt agcctgggat gaaccttagc taaccttata tcagatactg
     7021 cctctgttgc tttcataggg atgcctgtga tattaaatat ggaattctct tccgccctga
     7081 tggtcattgt tacattattt tcgtctgtgt tattaaaata gatcctgaca aatttttctc
     7141 ctcccgtaag attttcatcg ttcactgtca gaatataccg cggctttatg gttgtaactt
     7201 ctccctcgtt gtttttaagc tcaaggataa aagcatctga atgaaccgag cttaattttt
     7261 gggaatcgcc gtaaacttcg ccgctgaaag atacatctac atattggccg aaagagtcaa
     7321 ccgggataac cttgctgaca tgttcaattt ttaaccccgg ggcgggaatc ttgaattctc
     7381 cggatgattg gtaaaggagc atgtaacgca tttctccctg tatatagctg tagaccggat
     7441 gttcgttaaa atactggaag gaaattgttt catctcctgt gtattctcct gtaacctcaa
     7501 agaatgcccg gattgttttc tctccgccga ccaggggtga tgcttccaat tctatttcat
     7561 ggttcggctt tctaaaagat tttctaatta cttgcaaact tttatccgga tccttgaatg
     7621 agatggcaaa acattctttt tgtattgggc cgtcccagct ccaatcataa ctgtacaccc
     7681 cataattaaa ggatatgtcc agataattca ttcctccggg tgagtatatt ccggtaaata
     7741 ccggcaggtt gggctccggg tggaaaaact gggtccctag ggtcaatatg gtttttttgt
     7801 ccgaggatac aataaatcga gaatcgtagg ggctaatttg gaatttatca tccccgttag
     7861 aaaggccggt ttgcttcaaa aagaccctca ctttttttgc tccgcctaca aggggtgatg
     7921 cctcctccgg cgttgcccca tcagggtttc ttaccccaat aatttccata tgggaactgc
     7981 tgcccatcaa atttatggca tttattttaa aagctgcttt tgtcaaaggc ccccaggtat
     8041 ctaaaacata gtagaacaca ggtgtgttga aggtaatgtc aacatacagt cccggctctg
     8101 ttacaaattc cgaggaaact atttcaggtg tgggcggcag tacatgaata tcttcaacaa
     8161 caattgcata gtgcgccaaa acccctgttt catcctcaac atgactgaca cgtatgctga
     8221 aggtttcttc ccccgatggg ctgccctgaa gctccaggaa cattcttacg ctgttttctc
     8281 ctccttcaag gggggttgca tccgcttcaa ttttgctgtc aggctttttg agggatatta
     8341 tcttgatttt ttctaaggta ccgcctgcag ccagtgtaat gaaaaagtta tccgccgtaa
     8401 caggacttcc gtcactatta tatatcccct tattgaaaat aatgtccaaa tatctgttat
     8461 aggattccat ttcggcagac actattttaa tcctcatatc atggagagtt aaatcatctg
     8521 ttgaggcatt tttgatgata ctgccccagg aattgtatat ggggtggtca tattttgagg
     8581 ctagaatatg tactttatca tcccctggat gctgaagtat tttaaggaag aaccgcactg
     8641 ctgtttctcc tcccttgaga gggcttgctt cgttttcatt agtgctgtcc ggctttttaa
     8701 tggagctaaa ctgaactatt gtagaatctc cacttaaata gaagttgttc aggttaacag
     8761 tagtttttcc gtttgtatcc ccgtatactc ccgtgctgaa atatacatct atgtaactgt
     8821 tgtcttcagc tagctctgca gataacatat tcaattcccg ctgattgaag tatattactc
     8881 ctgtagtttt atcaatctgc atggctgttc ccctttggtc ataaagggaa cttccatccc
     8941 ttggtccgat gattatgttg tcattttttg tttcattccc tgataacgcc agaaaaaatc
     9001 taacgacagt ttctcctcct gtaaggggcg ttgctgatcc ttcacttgta ttgtcaggct
     9061 ttttgaccga gactatttcc gcctgggtga gtccgcagcc ctcagtatcc aggttcagac
     9121 ttaagttaaa gtctcctttt tctatagggc taagtccatc aggggagcca tgaattcccg
     9181 tgctaaaatg cacatctaca taggtgttgt ctattgccat ttctgtcttt gtgattctcg
     9241 gaatgtggtt taaattgatt atgcctgtgg tttctgtttc gtgcatgcaa agccctgaat
     9301 aggaaaatat tgtgctgtcg ccccgggctt taatttctat ggtttcctcc cctgttgcga
     9361 gtttggggat atataggaat acccttacgg ttttttcgcc gcctgttaag aaggatgctt
     9421 gttctatctc tgcagcgtca ggcttcctta catcagctaa gtctacgatt gtggcgtttc
     9481 cgccattggt ttcattgatc agggtaaaat cttctctgtt tacaggattc atatggtgac
     9541 tgtccccata aactccggtg ttgaaggtaa tgtctatata nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn atcaccatac acaggagtgc tcagttccat gtcaatatag
     9721 cttaaatccg ctgcaggagt aaccttgatt attgccggaa tcccgtcgaa ctttatattg
     9781 ccagttcttt ccgtgaagta gttaatagga ttccccacaa agttgtatat gggattgtca
     9841 atagtcgacc caataaaaca ctcctccgtg ccacttggtg tacctgttat atcaaggaaa
     9901 accctggctt ctgtttctcc tccttccagt ggggacgctt cttcacttac tgcactattg
     9961 cattttgtga ccttatgtat agatgcatta tatgcatttc caccgttgct atagaagcct
    10021 aagtggagat gttcccttgt tatcggactt gtatggttta tatcaccata gactccgtaa
    10081 tcaaagctta tatcaatgta gctgttgtca tttgacaatt gggcggatag tattcttgga
    10141 aaattaggtt taagcttaat catccctgtt gtagttatat ctgtcatgga ctttccactc
    10201 tggctgaaga tggattggct gtcagccgga tatatttcaa tagcttcgtt tgttcctccg
    10261 ccgtcaggtg agcctgcggc agggccggat aatgccagaa aaattcttat atgggtctct
    10321 cctcctgata agggagaggc tgagttttcc atttcgctgt ccgctttctt tacagatatt
    10381 attgttatgt tttcagcatg tccgttgttt ttgtagaagt ttagctggaa atctgattta
    10441 tcaacaggag tgctcccgtc agctgcgcca tatactccca tgttgaagtt gacatctaca
    10501 taggtgttgt ttggtcctac ggttgaggat gtaaacaagg gcagtacatt caggttggtg
    10561 gtatgctccg gttccatagt gaaatatggt tggccgcttt tgctgaatac gccctccacc
    10621 tgtattgcta ctgtttcagt accatttggc tgtccttcgc tcaaatccag gaacattctt
    10681 ataacccttt cgccaccttc cagatcagtg gcttcggatt ctatagtgct gtcgggtttt
    10741 tttaatgata atatagtaac atcttcaata tttccttcgt tttgctcata aaccaagcgg
    10801 atgttcatgc tggtaacagg gcctatacct atgcgttttt tcccaataaa atccatatcc
    10861 ccgtagacat ctgtgttaaa gtaaatgtca aggtagctgt tgtcctctgc caggtgaatt
    10921 ccaatgaacc ttatttccgg cggatttagc tggattatcc cggtagtgct gccctggagc
    10981 aaagcaattc cttcgcgaga atatacggcc tcatcacttg aggaaccgat tacgattgtt
    11041 tcttctccgt tagggttccc ctctatttcc agtataaacc ttattattat ttctccacct
    11101 gtgagagctt cctctgtctc aggatttatg atggaggttg ctcttatgcc cgttgccgtt
    11161 cctgtgtttt gtgagaaggt gagatcgaag tcttctattt ccacggggac tgagtgactt
    11221 aagtccccgt atactcccat attgaattcc acgtcaatat agctgttatc tggggcgata
    11281 tatgtagata ttagttttag tgaggcaggt tttagcctta ccggacctgt ggtgcgtcta
    11341 tccgtcatgt aagttccttc tgagtcaaat atttcatact cttccgggat atttacttct
    11401 attgtttcct cgccgcttgg agtgcctgtg attgaaaggc tgactctgac tactgattct
    11461 ccgcctaata agggggtacc atcctgtttt gttacggatg ttatggctgc acctgctgcg
    11521 tcccctccat tattttcaaa aaccaattca aaagcgtcat tagatacagg ggtcatcccg
    11581 gtgccgtaga ttcctgtgct aaaatatatg tcaatatatt gattggtgct gtccagataa
    11641 gaatgtatta ttctgggtct tgctataaga ctcgtcccgt taggggtatc ggctattccc
    11701 cagcttgtga gcgtattgtc gggttttagt gctgcagcac ctgtctgctg gcatgctata
    11761 gccattgccc ctgcggcttc tggaggtggt gccagtgctc ccgaatggta gccccaggct
    11821 atgatggtcc cgtctgcttt caggacgata gaatcgaagt atgagccatc tgctgctact
    11881 acattgtcga ggtattctgg aactgatatc tgaccaaaaa catcatatcc ccatgcaata
    11941 accgtaccgt cttttctaat tgcaaggtta tgcccccagg gagcagctat ggctgctaca
    12001 ttagcagttc caggatctgt aagctcatag ggaatctgaa gctgattcca gctgttattc
    12061 ccccaggcaa cgacagttcc gtcagcttta agagcaagag aatgaaatcc tcctgctttt
    12121 atagatacaa catttgcact ctcagggttc tggatgttct caggaacatc gtggttatat
    12181 gaattgtcat cgggggtata tccgccccag ccaacaacag ttccgtcagc tttaagagcc
    12241 aggctatgag agccgccagc tgccacagca atgactcctg aaaggcctgc aggcacatca
    12301 ctctgtccat aagtattatc accccaagcg acaacaatac catccgccct taaagcaaga
    12361 gcatgataag cacctgcgga aacctcaata acatcaacca ggtcataggg aacgttgcac
    12421 tgaccttgag tattatcacc ccaggcagca accctgccat cagaggtaat ggcaagagta
    12481 taatccggtc cgctgcttat ttttttcata taaccgctat gggaaaggat aagaccgcta
    12541 atgtctgtgg ttgcaaatgc agctgtaaat ccggcttcgt ccgaggcttt gtcgtcggat
    12601 ttgttattgg atttgttacc gattgtatct tcactacctt cagtactgct gtcaccttta
    12661 tcgctggctg ttccgttatc tgtgccagtt tctccttttg aatctgaacc agaatctgag
    12721 tcatccaggt catttgaatc atcagtatca tctgaatcat caatatccat atttgtgtca
    12781 ttattggtat tggtattcat atttagaatg tcttcgtcgg aaaagcctga agataagttt
    12841 aattggccta ttacgttact cgctgcatgt accgcacttg aagccagagc cgctaaaggc
    12901 agctgagtaa cagtaaaagc agtgataagg acgattgcta aaagcttttc tagttttctc
    12961 atacattttt ccccctaata aatacttcaa atttaaatat ttcatagttt agttaacatt
    13021 agcacgattt caatgtgaag atactttcac ttcttacacc tgttttattt taataattca
    13081 attagagatc gcataaacat tttttttatt taattctaca attttccact taaccaccac
    13141 ttaacaaata aaaaagcagc atatgaacaa gtttttatca tgtgccgcat tcaaaatttt
    13201 cctgtatgaa gtttcacaag ggtgcattta tcaaatctac cttatgagct ttttgaaagc
    13261 tgcatccggc tccaacccaa actttttata caagccagtg cagtaaaggt tataatgctt
    13321 tgctgccaat gcccgttcat gtgcatgcag taaaacttca atcagccgat agttaagctc
    13381 tcggtggagg ggctcgtaaa acaggccttc ttttaaccat ttttcaactt tcgaataatt
    13441 tttaatcctt ttataatact ctgtcatttt aagcagcaat tcaatgaatt tttcctccag
    13501 cagtaaacgc tttcccgcta cccattcata gtcccatcct gaaaggtatt ctcctgcata
    13561 aagtgctaaa gcctcttcac attctaaaat attatcccgt ccgatgattc cggtattttc
    13621 tgcaaaagta caaaattgca gataatcaca gtaaaggtta tccatattta tacggtaact
    13681 tccatggtca tactggatag aaatctctat tccagaaaga agcagtgctt tttttacata
    13741 atgaagagtg gt
//
LOCUS       DAOSDD010000109        13737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1748_length_13737_cov_5.664011, whole genome shotgun sequence.
ACCESSION   DAOSDD010000109 DAOSDD010000000
VERSION     DAOSDD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13737
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1748_length_13737_cov_5.664011"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK733_10665"
     CDS             <1..528
                     /locus_tag="PK733_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ11040.1"
                     /translation="KQTPDIVLMDIRMPVMDGLAAAKSISSGTSVRPLILTTFDDDEF
                     IINAIKSGAKGYLLKNNPPERMKDAIKLVHDGGTVMQDAVMDKLKESIDGIDITKKKS
                     KGSIADTLFSRFSEREMEVIELISKGMSNKEIAGKMFISEGTVKNYITSVLNKTGLEH
                     RTQIAIYYLNTLRQA"
     gene            722..1660
                     /locus_tag="PK733_10670"
     CDS             722..1660
                     /locus_tag="PK733_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11041.1"
                     /translation="MEIVKIRELTKRYNGTVAVDNISLNINEGEIYGLLGPNGAGKST
                     TINMILGLISMDKGSITIIGKDIKKNSYETRGNIGIVPQDIAIYEDLTAIENVMFFAS
                     LYGLKGNILRGKAEEALEFTGLTDKAKAFPKEFSGGMKRRLNIACAIAHNPKLIIMDE
                     PTVGIDPQSRNHILQSVKTLNSRGCTIIYTSHYMEEVEEICSRVAIMDHGKIIAEGTK
                     EELISIITDKNTVTITVAVQAENIDIGTIKDIYGVSDAEIHGNTVIVSSESDINNLDK
                     VIMYFTSNGISIKNVETKIPDLETVFLTLTGRKLRD"
     gene            1767..2912
                     /locus_tag="PK733_10675"
     CDS             1767..2912
                     /locus_tag="PK733_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ11042.1"
                     /translation="MALLEILKKEFKHNLREKRGNIMMVLFPILLMLILGTALSGMFE
                     SNITFDDAKVLYSIEDEGNITEAFKSFMQQGENLGIGFEETENLNDGIEKIKNAGFSC
                     FIIVNNEDSVLRFYKNDRYNFEANFVESILQVFVGRYNAIMEVAKENPAYLGTILSQD
                     NIAIQQESKGFSTVKTLDRTRQPRAIDHYAVVMLTMILMYSSLTGFWSIKNEQNLKTG
                     NRLLCARVQKHEILLGKVLGSIIATLVQAFIVIFFSKFALKAYWGQHIGIVILIIISE
                     AIMAISIGTGIAFAIKNETISIGILNTIIPIMVFLGGGYIPLQVFRKTLLNLSAISPI
                     RWINQSIFRVIYNNDFSLVPLTVTMNFTLAAVFIILSSLLYRKESVK"
     gene            2912..4057
                     /locus_tag="PK733_10680"
     CDS             2912..4057
                     /locus_tag="PK733_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ11043.1"
                     /translation="MKNIIKLIKNILKVTFRNKGNYIVYLILPIIGITTSIGLYAASD
                     TKPLTLGIIDHDGDRISKDMGNAFTRAGNFKIVELDEDKLNDHLLKGSVNCVVVIPED
                     FAESIYSYNPKTLEIITLEGQETAIWLESFANIYIRNVYDLAKASNGDKSAFERMYEG
                     YMAQNLKLNVGRVDDKAKNRMITVSSLGFLIMFIMLGANMTTEMVLKEKRNRTYFRIC
                     SAPVSSREYILANILAGTLIIVVQVILLIVIMRRIFHIETYIPDYQLFIILTLFGILA
                     IGVGMLIVSFSKSSYQSGTLANLIITPTCMLGGCFWSVKFMPEVLQKASYFMPQRWVL
                     DAVETIQKGGSIRDVAMHLGILAAFAAVFFIVAAYKFSTSDDIRTFV"
     gene            4257..5033
                     /locus_tag="PK733_10685"
     CDS             4257..5033
                     /locus_tag="PK733_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOJ11044.1"
                     /translation="MALIHCNFTSETLGVASSMCAILPQPQYIPENKDISWKNGGYPV
                     LYLLHGLSDDHTIWQRRTSIERYVASMNIAVVMPAVNRSFYSDMAYGQKYWTFVSEEL
                     PFIVRNLFPISNKREDTFVAGISMGGYGAFKLALTHPERFAAAASLSGVMDVAAKIDE
                     LGHENNECRLIFGDNSEIRRTKSDLFYLAEKVAKSDGLKPKLFQCCGTEDFLYTDNIR
                     FRDFCKNLPLELTYEEETGTHEWGYWDKKIQRVLAWLNKE"
     gene            5133..6257
                     /locus_tag="PK733_10690"
     CDS             5133..6257
                     /locus_tag="PK733_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ11045.1"
                     /translation="MDKLFIGFSYLDYKQIIMFLIGGALIYLAVKKDYEPMLLLPIGF
                     GTILVNIPLSTVLEYEGTPGVLKILLDAGIKTELFPVLIFIAVGAMIDFTPLFVNPSM
                     LFYGAAAQFGIFATIIIVLLFGIDDLKVASAIGIIGAADGPTAIFVASKFAQKYLGPI
                     TVAAYSYMSLVPMVQPPVIKLLTTKKERMIRMEYKDAKVSKTVKILFPIFVTIIAGIM
                     APISVPLVGLLMFGNLIRESGVLERLSKAAQNELANIVTILLGVTIGSTMTAEDFLQP
                     TTLLILLAGLAAFIFDTAGGVLFAKFLNIFRKNKINPMIGAAGISAFPMSARVIQKLA
                     TKEDPTNFILMQAVSANVAGQLGSIIAGGMILAIVPLLLG"
     gene            6400..6549
                     /locus_tag="PK733_10695"
     CDS             6400..6549
                     /locus_tag="PK733_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11046.1"
                     /translation="MFEYLINGTNLQKALFVMLAGILGVFAVLILFFILIIVLVRVFP
                     EKEEK"
     gene            6712..7386
                     /locus_tag="PK733_10700"
     CDS             6712..7386
                     /locus_tag="PK733_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOJ11047.1"
                     /translation="MVLDFKLFLEVIRKRILMIMLLPIISAGLSILISFYFIEPKYES
                     SATFYLINKKSGYAFPGEYGEMLLNRQLIRDYTKILKSKSNMESIINKYKLEKYTPAE
                     LAKRISISFTDDTNVIEIKAYSEFPELAKVIADAAYNIFVQNLAEFTEVFDVKLVDDA
                     ELPEKPSNSNVLVNTAIAFLAGVLISVSMAVFLEYIDHTIKTREDVETYLGLTVLGRI
                     PVIERE"
     gene            7416..8156
                     /locus_tag="PK733_10705"
     CDS             7416..8156
                     /locus_tag="PK733_10705"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ11048.1"
                     /translation="MTKNIIQVVNRLSAQAEEAYKVLRTNIQFCELDRQIKTLTITSC
                     LPGEGKSTTSMNLGVSFAETGKKVLVVDADLRKPILMKHFDNTDFKGLSNYISGRVSL
                     DDIIYNTNVNGLHFITSGVKPPNPAELIGSAKFRDFLKIVREQFDLIIFDTPPLGSVI
                     DGVIIASQTDGTLLVIECNVVSYQTAQQVTKQLKMAEARILGVVLNKIGSKYYKGYNN
                     YYDFYENRNNTDRELMGRLREKRGVMRL"
     gene            8153..8998
                     /locus_tag="PK733_10710"
     CDS             8153..8998
                     /locus_tag="PK733_10710"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOJ11049.1"
                     /translation="MIDIHSHLIYEVDDGPATIKESIRMVLEAEKIGIQSIIATPHFH
                     ENIYSTEKVIYNFNELVHRTRDCGVELYLGYEVFINTLKNICMSGNMQENTTLKGIPQ
                     KDMSSADMTQKVTDRAGTAQRDSNQADMARKNASLAGITLGGSRYLLFELPFDSIPAY
                     SYETIYTLNLENIIPVIAHPERNRSFVRSFGSFISFIEKGCLLQVDAASIIGIYGTEV
                     RSFTKRLIKLKLANFIASDAHCSQDYIEWYVSAYNTVCRWAGIEYAEKLFYGNPMMIV
                     DQLKK"
     gene            9025..9645
                     /locus_tag="PK733_10715"
     CDS             9025..9645
                     /locus_tag="PK733_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ11050.1"
                     /translation="MKKISSNIEYIIDQKKLNKSVSGRYVIIKRICDVFLAALCIILF
                     LPAIVTAAAALKLNSYRTVFIYDYVYGQKGRIFKLYSFNVKTNSRRQSLNNLNDVGEI
                     IEKTGVKKLPYLFNILKGELSFVGPAPLSKTICNIDEHNSWYALRFSVKPGLICLYTI
                     TSGKCPDIDEMVRADLKYIKERNLLYDFKIFLAYIRIIAGNYRKLI"
     gene            9658..10266
                     /locus_tag="PK733_10720"
     CDS             9658..10266
                     /locus_tag="PK733_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOJ11051.1"
                     /translation="MENNDCFVHESSYVDEDVYIGKGTKIWHFSHIQKGARIGCNCTI
                     GQNVNIANNVCVGNNVKIQNNVSIYEGVELEDYVFCGPSMVFTNIKNPRCKYPQRGSE
                     YYMRTLVKEGASIGANATIICGNTIGKHALVAAGAVVTKDVPDYALVTGVPARITGWV
                     CECGQKLPGNLKNILDSNLDDNLVCKHCGRVYKEEYGQLSEE"
     gene            10402..11526
                     /locus_tag="PK733_10725"
     CDS             10402..11526
                     /locus_tag="PK733_10725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ11052.1"
                     /translation="MIPFIDLMEQYRRLKSEIGSGIQQVLEHGKYIMGPEVYELENRL
                     ADYVGVRHVISCSSGTDALLMPLMAWNIGKGDAVFTSTFTFIATAEVVSLLGATPVFI
                     DIDPGTWNIDPNSLEEKIRQTIAEGKLIPKVVIPVDIFGLPADYEAIESIAAKYGIKV
                     LEDAAQSFGGIYKGKKTGSFGNAAGTSFFPAKPLGCYGDGGAVLTDDDKLAGELRSIR
                     AHGQGGDKYNNIRTGINGRLDTIQAAVLLAKLNVFDMELLRRNEIADKYTRGLRGVVK
                     TQTIPDGCSSSWAQYSIAVGSYDEREYIREKLTVCEIPTGIYYPRPLHLQEAFSYLGY
                     SEGDFVVAEDVSKRIMSVPMHPYLPDETIEKIIEIIEISI"
     gene            11774..13099
                     /locus_tag="PK733_10730"
     CDS             11774..13099
                     /locus_tag="PK733_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ11053.1"
                     /translation="MKDCAGAKDGLLQKIHDKTAVAGVLGLGYVGLPLAVQKAKTGFK
                     TIGFDIQKHKVDMVNQGRNYINDIPDDDLSHVVSTGMLNATDDIAFIGLTDCIAICVP
                     TPLDIHRQPDLSYVINSAKAIAEYLHRGMLVVLESTTYPGTTEEVIKPLLESSGLKCG
                     EDFFLAFSPERVDPGNKLYKTKNTPKVVGGVTKDCTEVASAFYRNILEDGIVEVSSPA
                     VAEMEKILENTYRNINIALANEMAILCNKMGINVWEVIEAAKSKPYGFVAFYPGPGIG
                     GHCIPIDPFYLTWKAREFDYHTRLIEVAGEINSGMPEFICTRVMFLLNKRKIALNGAH
                     IFIIGVAYKQDIDDLRESPVLKVMEKLEEQGALLTIHDPFIPVFQHNGKEYRSEELSA
                     EKLKNADIVVITTAHSCLDYNFIQRNSTTIFDTKNAMKNAKDRDNVVLL"
     gene            13159..>13737
                     /locus_tag="PK733_10735"
     CDS             13159..>13737
                     /locus_tag="PK733_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ11054.1"
                     /translation="MAKYLVTGGAGFIGSNLAEHLVNEGYSVRVVDNLATGSFENIKS
                     FADKIEFICGDLADSKVAEKAVDGVDYILHQAAIPSVQRSINDPVSTNRSIITATVNL
                     FKAAVDCGSIKRIVQASSSSVYGDTPILPKREDMTPNPLSPYAVAKFTQECYAKVFYR
                     VYGLEVLSLRYFNVFGPKQNPYSLYSSVIPKFS"
BASE COUNT         4614 a         2060 c         2983 g         4080 t
ORIGIN      
        1 aagcaaacac ctgacattgt tcttatggat attagaatgc ctgtaatgga cggactggcg
       61 gctgcaaaat caatatcctc aggcacatct gtaagacctc ttatacttac aacttttgat
      121 gatgatgagt ttattattaa tgcaataaaa agcggagcaa aaggctattt actcaagaat
      181 aaccctcctg aaagaatgaa agatgcaata aaactcgttc atgatggcgg tacagtgatg
      241 caggatgcag taatggataa gctaaaagaa agtatagatg ggatagacat aaccaaaaag
      301 aaaagcaagg gaagcattgc cgatactctt ttttccaggt tctccgaaag agaaatggaa
      361 gtaatcgagc taatttcaaa aggaatgtca aacaaggaga ttgcaggtaa aatgtttatt
      421 tctgaaggca cggtaaagaa ttacattact tctgttttaa ataaaacagg acttgaacat
      481 cggacgcaga tagccatata ctaccttaat accttaaggc aggcataaat tttctttcaa
      541 gcgtttgaat attaagaggg gcattcctga ataattaaaa acatgactaa agtcatgtaa
      601 aaatagtgac ctattggact aataaaaatt agtccttttt tttataatgt aatcacaaga
      661 gattatgagc aacttcataa taacaaatag tacttattat gggataatga ggtgattata
      721 tatggaaata gtaaaaataa gggaacttac aaaaaggtat aatgggactg tagctgttga
      781 taatatcagt ctcaatataa acgaaggtga aatatatggg cttcttgggc caaatggagc
      841 aggaaaaagc acaacaatca atatgattct tggcctgatt tctatggaca aaggcagtat
      901 aactattatc gggaaagaca taaaaaagaa ttcatatgag actcggggaa acattggaat
      961 tgttccgcag gacatagcta tttatgaaga tcttacggcc attgaaaacg taatgttctt
     1021 tgcaagctta tatggactaa aaggaaatat tctcaggggt aaggcggagg aagctcttga
     1081 gtttacaggg ttaacagaca aagcaaaagc ttttccaaag gagttttcag gaggaatgaa
     1141 aaggagacta aatattgcct gtgccatagc acataatcct aagctaataa taatggatga
     1201 gccgacagta ggtatagacc ctcagtccag aaatcacata cttcagtcgg tgaaaaccct
     1261 taatagcaga ggatgtacaa taatatacac cagtcattat atggaggaag tagaagagat
     1321 ctgcagcagg gttgcaatta tggaccatgg aaagattatt gcagagggaa cgaaagaaga
     1381 acttatatca ataataaccg ataaaaatac tgttactatt acagtagctg ttcaagctga
     1441 aaatatagat ataggtacta ttaaggatat atatggcgtt tcggatgctg aaatccatgg
     1501 gaacactgta atagtcagca gtgaaagtga cattaataat cttgataaag ttatcatgta
     1561 ctttacatca aatggtatat ctattaaaaa tgttgaaact aagataccgg atctggaaac
     1621 agtatttttg acccttaccg ggagaaaact gagagattag gaagttttga gattaagcta
     1681 ggattaagat tagggttaga attagaattg gagctggaat tagggctgga attagaattg
     1741 gagctggagt tggagaggtg aatagtatgg ctttattaga aatattaaaa aaagagttca
     1801 agcataacct gcgggaaaag aggggaaata ttatgatggt gctgtttcct attttattga
     1861 tgttgatact tggaaccgca ctttccggta tgtttgaatc caatattacc tttgacgatg
     1921 ctaaggtgtt gtattccata gaagatgaag gaaatataac tgaagcattt aaaagcttta
     1981 tgcagcaggg tgaaaacctg ggaatcggct ttgaggaaac agaaaatttg aatgatggaa
     2041 tagaaaaaat taaaaatgca ggattttcct gctttataat tgtaaataat gaagatagtg
     2101 ttctaagatt ctacaaaaac gacagatata actttgaagc taactttgtt gaatcaatac
     2161 ttcaggtttt tgttggaagg tataatgcta ttatggaggt agcaaaagaa aatccggcat
     2221 atctaggtac aattctttca caggataata ttgccatcca acaggaaagc aaaggtttca
     2281 gcacagttaa gacattggac agaacccggc agcccagggc tatcgaccat tatgctgttg
     2341 taatgctgac aatgatatta atgtattctt ctcttaccgg attctggagt ataaagaacg
     2401 aacagaattt gaaaaccgga aatcgattgc tatgcgccag agtccaaaag catgagatac
     2461 ttctgggaaa agttttagga agtattattg caacacttgt tcaagcattt attgttatat
     2521 ttttcagcaa gtttgcttta aaggcatatt ggggtcagca tattggtatt gtaatattga
     2581 ttattataag cgaggctata atggctataa gcatagggac aggaatagcc tttgcgataa
     2641 aaaacgaaac catatcaata ggtatattaa acactataat accgataatg gtctttcttg
     2701 ggggaggata tatcccactg caggtgttta ggaagacact cttaaatctt tctgctatat
     2761 cgcctataag atggataaac caatctatat tcagggttat atataataat gacttttcac
     2821 tggtgccttt aacagtgaca atgaacttta cgcttgctgc agtatttatc attctgtcat
     2881 cgttgctata tagaaaggag tctgtgaagt aatgaaaaat attattaagc ttataaaaaa
     2941 cattctaaag gtaactttca gaaataaagg gaattatatt gtctatctaa tacttcctat
     3001 tattggcatt acaacatcta tcgggttata tgccgcatcg gatacaaaac ctttgacgtt
     3061 aggtattatt gaccatgacg gcgatcgaat ttccaaagat atgggaaatg catttaccag
     3121 ggcaggcaac tttaaaattg tggaactgga tgaggataag ttaaatgatc acctattaaa
     3181 aggcagcgtt aactgtgttg tagttatacc tgaggacttt gctgaaagta tttacagcta
     3241 taacccaaag acacttgaaa ttataacgct ggaagggcag gaaactgcta tatggctgga
     3301 gagttttgca aacatatata tcaggaatgt atatgatctt gcaaaagctt ccaacggaga
     3361 taaaagtgct tttgagagaa tgtatgaggg atatatggct caaaacctga aactaaatgt
     3421 tgggagagtt gatgataagg ccaaaaatag aatgattacc gtttcaagcc tcggatttct
     3481 tataatgttt ataatgcttg gcgctaacat gacaacagaa atggtactaa aggaaaaaag
     3541 gaacaggaca tatttcagaa tttgctcagc tcctgtaagc tcaagagagt atatattggc
     3601 aaatattcta gccgggacat taattatagt tgtccaggta attctgctaa tagtaataat
     3661 gagaagaata ttccatatag aaacctatat tcctgattat cagcttttca taatattaac
     3721 actctttgga atattggcta taggagttgg aatgcttatt gtatcctttt caaagagttc
     3781 ataccagtca ggaacccttg ctaatttgat aataactcct acatgtatgc ttggaggatg
     3841 tttctggtcg gtaaagttta tgcctgaggt cttacaaaaa gcttcatatt ttatgcctca
     3901 aagatgggtg cttgatgctg ttgaaacgat acaaaaaggc ggcagtatca gggatgttgc
     3961 catgcatctc ggcatacttg cagcttttgc tgcagtattc tttattgttg cagcatataa
     4021 attcagtaca agtgatgata taaggacgtt tgtgtgagag gggaagaggt gtattccctc
     4081 cgcttttctg tttgtatctc tattgacata ttacctttct agtgagaaaa taccattatc
     4141 atcagtacag tatttgcagt agcctaatac ttaaaaaccc aggtgcacac ttgcacctga
     4201 aactggtatg acttaatgtc ataccaaatt ttattattac tgaggaggaa atattcatgg
     4261 ctttgattca ctgtaatttt acgtctgaaa cacttggcgt tgcttcatca atgtgtgcaa
     4321 tcctgccaca accccagtat attcctgaaa ataaggatat aagctggaag aatgggggat
     4381 atccggtatt atatctgctc catgggttat cggatgacca taccatatgg caaaggagga
     4441 cttcaattga aaggtatgta gcttctatga atattgcagt tgtaatgccc gcagttaaca
     4501 gaagttttta tagcgatatg gcttatggcc agaaatactg gacttttgtg agcgaagaac
     4561 tgccttttat tgtacggaat cttttcccca tatccaacaa gcgggaagat acttttgttg
     4621 ctggcatttc catgggaggg tatggagcat tcaagcttgc tttaactcat ccggaaagat
     4681 tcgcagctgc tgcaagcctt tctggcgtca tggatgttgc agcaaaaatt gatgagttag
     4741 gtcatgaaaa taatgagtgc aggttaatat ttggtgataa ttctgagatt agaaggacta
     4801 aaagcgacct cttttatctg gcagagaagg ttgcaaaatc tgatgggcta aagccaaagt
     4861 tgtttcagtg ctgtggtacg gaagatttcc tttatactga taacataagg ttcagagatt
     4921 tttgcaaaaa cttacccctt gaactgactt atgaggaaga aaccgggaca catgagtggg
     4981 gatattggga taaaaaaata cagagagttt tagcatggtt aaataaagaa taaagagatc
     5041 ttaagttact atgtgtaact ataaactata ctatctatac tatagttaac tatgagtatt
     5101 ttgaactcat accgtgaatt ttggaggttt ttatggacaa gctttttatt ggattttcgt
     5161 atttggatta taagcagata ataatgttcc ttattggtgg agcacttatt tatcttgctg
     5221 ttaaaaagga ttatgagcct atgttgctct tgccgatagg ttttggcacc atattagtta
     5281 atattcctct ttcaactgtg ctggagtatg aaggtacacc gggagtatta aaaattctcc
     5341 tagacgcagg aataaagact gaactttttc cggtacttat atttatagca gtaggggcaa
     5401 tgattgactt tacacccctt tttgtaaatc catcgatgct tttttatggt gctgcagccc
     5461 agttcggtat atttgcaact attattattg tgctgctatt tggcatcgat gatttaaaag
     5521 tagcatcggc aataggtatt attggtgctg cagacggacc aactgcaatt tttgtagctt
     5581 cgaaatttgc acaaaaatat cttggaccga tcacagttgc cgcctattca tacatgtctt
     5641 tagtaccaat ggttcaacca ccggtaataa aacttcttac aactaaaaaa gaacgtatga
     5701 taagaatgga atacaaagat gcaaaggttt caaaaactgt taaaatacta tttcccattt
     5761 ttgttaccat aattgcgggt ataatggcac ctataagtgt tccgctggta ggacttttga
     5821 tgtttggtaa tcttatccgg gaaagtggag tacttgaaag gctttccaaa gctgctcaga
     5881 atgaactggc caacattgtt acaattcttc tgggggtgac tattggctcc actatgacag
     5941 cagaggattt tttacagcca actacactgt tgattctttt agcaggtctg gcagctttta
     6001 tttttgacac ggcaggtggg gttttgtttg caaaattcct taatatcttc cggaaaaaca
     6061 aaataaaccc catgatagga gctgcaggaa tatcggcatt tccaatgtct gcaagggtta
     6121 tacagaagct tgcaacgaaa gaagatccca caaattttat tttaatgcag gcagtaagtg
     6181 ccaatgttgc aggacagctt ggctcaataa tagcaggggg aatgatactt gctattgttc
     6241 cattgctgtt agggtagctt atcattgctg ctgttttggt taaacgggtt ttattagttt
     6301 tgtactttaa atttagaggg tgcagctgat ataacgaatg taacaaacat aattaatata
     6361 attaatgtaa tgaatgtaaa gaaaggaatg catattaaca tgttcgaata tttaattaac
     6421 ggtacgaatc tgcaaaaagc actttttgtt atgcttgctg gtatcttggg ggtatttgcg
     6481 gtgcttatac ttttctttat tttaattata gtattagtca gggttttccc tgaaaaggaa
     6541 gaaaaataac taaaaaagca gattgcaaaa caaagttctt ctataataaa gttcttctat
     6601 attgggtata tttatgaaca cccatgtgga agaacttttt ttcgtcacac ttaaccctga
     6661 taaaaagtat aatatattac tgattaaagt agcaaagaga aagggaatag tatggtgtta
     6721 gattttaaat tatttcttga ggtaataagg aaaagaattt tgatgattat gttgcttccg
     6781 ataatatcag caggactttc cattttaatc agcttctatt ttattgagcc aaagtatgaa
     6841 tccagtgcta cattttatct tataaataaa aaaagtggtt atgctttccc gggggaatat
     6901 ggagaaatgc ttcttaacag acagttgata agagattata ctaaaatcct caaaagtaaa
     6961 tcaaatatgg aaagcattat caataaatat aaattggaaa agtatacacc agcagaattg
     7021 gcaaagagga tcagcataag ctttactgat gatactaatg ttattgagat aaaagcatat
     7081 tctgagtttc ctgaattggc aaaagttatt gctgatgctg cctataacat atttgtgcaa
     7141 aaccttgcag aatttacaga agtatttgat gtaaagctgg ttgatgatgc ggagcttcct
     7201 gaaaagccct caaattccaa tgtacttgta aatacagcta ttgctttttt agctggtgtt
     7261 ttaatatccg tgagtatggc agtattttta gaatacattg atcatacaat aaagacccgt
     7321 gaggatgtgg aaacgtatct cgggcttacg gtacttggta gaattcctgt aattgaacgg
     7381 gagtaatggg cctagaatag acgggggttg aagaaatgac aaaaaatatt attcaagttg
     7441 ttaataggct gagtgctcaa gcggaagaag cctacaaagt gttaagaaca aatatacaat
     7501 tctgtgagtt ggataggcaa atcaaaacat taactataac aagttgtttg cctggagagg
     7561 gaaagtcaac aacatcaatg aatcttggtg tttcttttgc tgaaactggt aaaaaggttc
     7621 tggtggttga tgctgatttg agaaagccaa tcctgatgaa acattttgat aatacggatt
     7681 ttaaggggtt gtccaattat atatcagggc gtgtctccct ggatgatatc atttataata
     7741 cgaatgtaaa cggactgcac tttataactt caggagtaaa accccctaat cctgctgaac
     7801 ttataggctc agcaaaattt agagactttt tgaaaatagt cagagaacaa tttgatttaa
     7861 taatatttga tactccacct cttggtagtg taatagatgg agtaattata gcttctcaaa
     7921 cagatgggac actacttgtc attgaatgta atgtagtaag ctatcagact gcacaacagg
     7981 taaccaagca gctgaaaatg gctgaagcgc gaatattggg tgtagttctg aataaaattg
     8041 gcagtaaata ttataaaggg tacaacaatt attatgattt ctatgagaac agaaataata
     8101 cagacaggga attgatgggg agattaaggg agaaacgagg ggtaatgagg ttatgattga
     8161 catacacagt catcttattt acgaagtaga tgacgggccg gcaactataa aagagtccat
     8221 aagaatggta cttgaagctg aaaagattgg tatacagtcc ataatagcta cacctcattt
     8281 tcacgaaaac atctatagca ctgaaaaggt tatctacaac tttaatgaat tagttcacag
     8341 gacaagagat tgcggcgtag aactctattt gggatatgaa gtatttatta acacattgaa
     8401 gaatatctgt atgtcaggca atatgcagga gaatacaact cttaaaggta taccccagaa
     8461 ggatatgagt tctgcagaca tgactcaaaa ggttaccgat agggcaggca cagctcagag
     8521 agattcaaat caggcagaca tggctcggaa gaatgcaagt cttgcaggta taaccctcgg
     8581 aggctcgaga taccttttat ttgaactgcc ttttgatagt atacctgcat acagttatga
     8641 aactatatat actttaaatc tagaaaacat tattccagta attgcacacc ctgagcgtaa
     8701 cagaagcttt gtcagaagct ttgggtcatt tataagtttt atagaaaaag gctgtctgct
     8761 gcaggttgat gcagcaagta taattgggat ttatggtact gaagtgagga gttttacaaa
     8821 aaggctgata aagcttaaac ttgcgaattt tattgcgtct gatgcccatt gctcccagga
     8881 ttacattgaa tggtatgtat ctgcttataa cacagtatgc aggtgggcag gtatagagta
     8941 tgcagagaag cttttttacg gtaatccaat gatgatagta gatcaattaa aaaagtaata
     9001 aacaggcggg aggttaagtt atttatgaag aaaatatcat caaatataga atacattatt
     9061 gatcagaaaa agctaaataa aagtgtttct gggagatatg taataattaa gagaatatgt
     9121 gacgtttttc tagctgcatt atgtataata cttttcctac cggctattgt tactgctgct
     9181 gcagctttaa agttaaattc atataggact gtgttcattt atgactatgt ttatggacaa
     9241 aaaggaagga tttttaaact atatagtttt aatgtcaaaa ctaatagccg cagacaaagc
     9301 ttaaataatt tgaatgatgt aggagaaatt atcgagaaaa caggagtaaa gaagctgcct
     9361 tatttattca atatacttaa gggtgaattg agttttgtag gacctgcacc cctatcaaaa
     9421 acaatatgta atattgatga gcacaattcg tggtatgctc ttcgtttttc agtaaaaccc
     9481 ggcttaatat gtttatacac tataacttcc ggaaaatgtc cagatattga tgaaatggtg
     9541 agagctgatc ttaagtatat aaaagagaga aatttattgt atgattttaa aattttctta
     9601 gcatatattc gcattatagc aggtaattac agaaaactta tataagaggt gggcaatttg
     9661 gaaaacaatg attgcttcgt gcatgaaagc tcatatgtag atgaggatgt ttatataggc
     9721 aaggggacta aaatatggca tttttctcat atccaaaaag gtgcgagaat cggatgtaat
     9781 tgtaccattg ggcaaaatgt taatattgca aataatgtct gtgtaggaaa caatgtgaaa
     9841 atacagaata atgtatcgat ctatgaagga gtagaacttg aggattatgt tttttgtggt
     9901 ccttccatgg tttttacaaa tattaaaaat ccccgctgca agtatcctca aagggggagt
     9961 gagtactata tgagaactct ggtgaaggaa ggggcttcaa taggtgcaaa tgcaactata
    10021 atatgtggta atacaatagg aaaacatgca cttgttgcgg caggagcagt ggttacaaag
    10081 gatgtacctg actatgccct ggtgacaggt gttcccgcaa gaataacagg gtgggtatgt
    10141 gaatgcggcc aaaagcttcc aggtaatctg aaaaacatac tggatagtaa cctggatgac
    10201 aacctggtat gtaaacattg tggaagagtt tataaagaag aatatggaca attaagtgag
    10261 gaataaggca gaaaaaatca gagaaaagtt agtgaaaaag ccggagaaaa gttggcgaaa
    10321 aatcagtgaa aaagttggta aaaagccagt gaaaggtcag ttggaagtca gttaaaaaat
    10381 cagcgaaagg aatgattagg aatgataccc tttattgatt tgatggagca gtaccgcaga
    10441 ctaaaaagtg aaattggttc aggtatccag caagtgctgg aacatggtaa atatataatg
    10501 ggaccggaag tgtatgagct tgagaacaga cttgcagatt atgttggtgt aaggcatgtt
    10561 attagttgtt caagcggtac cgatgccctg cttatgccat tgatggcatg gaatataggt
    10621 aaaggtgatg ccgtatttac ttcaacattt acttttatag ctactgcaga agtagtttcc
    10681 ctacttggag caacaccggt ttttatagat attgatcccg gaacatggaa tatagatcca
    10741 aactctcttg aagaaaaaat caggcaaact attgctgaag gtaaactaat accaaaagtt
    10801 gtaatacctg tggacatttt tggattgcct gctgattatg aagcaataga atcaatagct
    10861 gccaaatatg gaattaaagt tcttgaggat gctgcccaaa gttttggcgg aatatataaa
    10921 ggtaaaaaaa ccggaagctt tggtaatgct gccggaacat ctttcttccc tgcaaaaccc
    10981 ttaggctgtt atggagatgg tggagcggtc cttacagatg atgataaact ggcaggcgaa
    11041 ttgaggtcaa taagggctca tgggcagggt ggagataaat ataataatat tagaaccggt
    11101 ataaatggaa ggttggatac catccaggct gctgtactac ttgccaagct taatgttttt
    11161 gatatggaat tattacgaag aaatgaaatt gcagataaat ataccagggg tttgagaggt
    11221 gttgtgaaaa cacagacaat accggatggc tgcagttctt catgggctca gtattctatt
    11281 gcagtcggga gttatgatga aagggagtat atcagggaaa aacttacagt gtgtgaaatt
    11341 cctaccggta tatattatcc caggccctta cacttacagg aggcttttag ttatctgggc
    11401 tatagtgagg gagattttgt tgtggcggag gatgtaagca aacgaataat gagtgttcct
    11461 atgcatcctt acttgccaga tgaaacaata gagaaaatta ttgaaataat tgaaatatcc
    11521 atatgagcta tgagctaatc agggaaccgt tcgtaacagc ttaaaatggg gaaagtggag
    11581 ggctgtcccc taccctaaaa gtcggaaagt ggaggactgt cccccaaaat tggaaagtgg
    11641 gagactgtcc ccggagacgc aaggtaagag ccatccctgc tacatactgt agctaagaga
    11701 ggttgaatgt aatatgtgtg atatagatgg aatggtagat tatgttggta gaaaagtaga
    11761 ttgtgtagat agaatgaaag attgtgcagg cgcaaaagat ggtttgctgc aaaaaataca
    11821 tgataaaact gcagttgcag gggttcttgg attaggttat gtagggcttc ctcttgctgt
    11881 gcagaaggca aaaaccgggt ttaaaacaat aggttttgat atacaaaaac ataaagtgga
    11941 tatggtgaat caggggagga actatataaa tgatataccg gatgatgatt tatctcatgt
    12001 tgtaagcacc ggcatgttaa atgcaacaga tgatatagct tttattggct taactgattg
    12061 tatagcaata tgtgttccca caccgcttga tattcaccgg cagccggatc tttcatatgt
    12121 aataaattct gctaaagcta ttgcagaata tcttcacagg ggtatgcttg tggtcctgga
    12181 aagcacaact taccctggaa ctaccgaaga ggtaataaag ccgctgcttg agagctcggg
    12241 acttaaatgc ggagaggatt ttttccttgc tttttccccg gaaagagttg atccaggtaa
    12301 taaattatac aagactaaaa atacaccaaa ggtagttggc ggggtaacga aagactgtac
    12361 agaggtagca tctgcatttt accgcaatat actggaagat ggtattgttg aggtatcttc
    12421 ccctgctgtg gcggagatgg aaaaaatatt agagaacact tacagaaata taaacatagc
    12481 acttgcaaat gagatggcta ttttgtgcaa taaaatggga attaatgtat gggaagttat
    12541 agaagcagca aagtcaaagc cttatggatt tgttgcattt taccctggcc ctggaatagg
    12601 aggacactgc atccccatag atccctttta tttaacgtgg aaagcccggg aatttgatta
    12661 tcatacaagg cttatcgaag tagcaggaga gatcaactca ggtatgcccg aatttatttg
    12721 cacaagggta atgtttctcc ttaataagcg taagattgcg ttaaatggtg cacatatttt
    12781 tattatagga gttgcttata agcaggatat tgatgattta agggaaagcc cggttctaaa
    12841 ggttatggaa aaactagagg aacagggagc tctgcttaca attcacgacc ctttcatccc
    12901 tgtatttcaa cacaatggca aagagtacag aagtgaagag ctatctgctg aaaaactgaa
    12961 aaatgctgat attgtagtta ttacaacggc acatagctgt ctggattata attttataca
    13021 aagaaattct accacgattt ttgatacaaa aaatgcaatg aaaaatgcaa aagataggga
    13081 taatgttgtg cttttataag cctgagcaag tcaattcaag tcacttatta aataattagt
    13141 ggaggttaga agttatatat ggctaaatac ctggtaacag ggggagcagg ttttattggt
    13201 tcaaacctgg ctgagcacct ggttaatgaa ggatattcgg tgagagtggt agataacctg
    13261 gcaacgggtt catttgaaaa cattaaatct tttgcagata aaattgaatt tatctgtggt
    13321 gatttagctg atagcaaggt agcggaaaaa gcagtggatg gtgtggatta tattttgcat
    13381 caggctgcaa ttccttcagt acaacggtca attaacgacc ctgtatctac taatagaagc
    13441 attattacag ctacagtaaa cttatttaaa gcagctgttg actgtggcag cataaaaaga
    13501 atagttcagg cttcttcatc atcagtatat ggtgatacgc ctatattacc taaaagggag
    13561 gatatgactc cgaatccttt gtcaccatac gcagtagcta aatttactca ggaatgctat
    13621 gcaaaagtat tctatagagt gtatggactt gaagtattat cattaagata ttttaatgta
    13681 ttcggtccta aacagaatcc atattcctta tattcatcag ttataccaaa attttca
//
LOCUS       DAOSDD010000110        13587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1766_length_13587_cov_5.605306, whole genome shotgun sequence.
ACCESSION   DAOSDD010000110 DAOSDD010000000
VERSION     DAOSDD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_13587_cov_5.605306"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK733_10740"
     CDS             <1..222
                     /locus_tag="PK733_10740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ11055.1"
                     /translation="WSGGYGKLVIIDHENGYTTYYAHNDTINVKKGQRVARGDTIGTA
                     GSTGNATGPHLHFEVRKNGVPVNPLNYVK"
     gene            475..888
                     /locus_tag="PK733_10745"
     CDS             475..888
                     /locus_tag="PK733_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11056.1"
                     /translation="MFRNTFKDIFKGKYGIDNLTLTLVFAALVFYNIKYLWVIGIVLS
                     GYAIFRTFSKDINKRYQELQKFNMLVNKIWQYINRSIQWIRFQYGVCRTRLQQRKQYV
                     FFKCPKCKKTLRLPKNKGKLQVTCPVCKYEFFKKT"
     gene            1041..2120
                     /locus_tag="PK733_10750"
     CDS             1041..2120
                     /locus_tag="PK733_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11057.1"
                     /translation="MTEFITIGKSKQFYDFLKNEILSGEYKAGDKFPSIRDLAEKYSV
                     SKTTVNLVISNLVNEKLLYVEQGKGTFVSDKKPDSRSSKKMIGVMLFDFRYENNVEAP
                     IFNSIQENLKDDYFLIPYNSYDNNEIFYKGIKGLMELDVDGMILVPPTMEDHDADYIK
                     SLITKDIPIVFINRRIPGIKGDFFSMDFEKGVYKAAKYIIDSGKKRIAMFKNKSPSLQ
                     EQMYRGFVAAHKEAGLEFKDEYMLEWPYWSKMGMGGDPKIAEISLKKVINEIDGLIAS
                     DYFIYKNRNVIYESGKVIPSDISIVGLNDSAYSRFMNPKLTAIPFLSEEIGVQAVKTI
                     MERIEGKKKAEVNMWLEPDIIIRSS"
     gene            2299..3237
                     /locus_tag="PK733_10755"
     CDS             2299..3237
                     /locus_tag="PK733_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ11058.1"
                     /translation="MINVKFKGIMPAIISPVNEDGSIREKELRRLINWQLAAGCSGFY
                     ICGATGEGTVMKPEARKAMAEIAVDEVKGRGCIIDHIGAIDLKTAADLARHASDIGVD
                     AISSVPPFFYTYGENEIMNYYQAVSDASDVPILMYASPLAGTNITAEIVEKMLSIKNM
                     IGLKWTSYNYYEMRKIKELNNSDINVINGPDETLLCGLAMGADGGIGATYNPMPKIFV
                     DIYNNFTEGKIDEAQKAQFKANKVIRILLKYGVLCGVKDMLDMLGFDVGYCTYPLKRF
                     TPSEQEAFRAELKAINFVEKYLHNYVDNHADNYVDN"
     gene            3298..4077
                     /locus_tag="PK733_10760"
     CDS             3298..4077
                     /locus_tag="PK733_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ11059.1"
                     /translation="MRDLFSMENKAVVITGGAGYLGSAMVEGLLEFGAKVMVADIAKK
                     KPEEIVNNGKSYTNLYSIACDISRTESIRELFKNAREICGEIDVLINCATYGAGGSIE
                     TMSDEAWLKGVDGSLGTVFRCTREVIPYMAENGGGSIVNIASMYGMVSPDPRIYGTSG
                     QNNPPNYGAAKAGVIQLTRYCAGHLADKNIRVNSVTPGPFPSPVKLPPEEFMNNLKNK
                     TMLGRVGKPRDIVGAVILLASDASSFMTGSNIVVDGGWTAW"
     gene            4182..4394
                     /locus_tag="PK733_10765"
     CDS             4182..4394
                     /locus_tag="PK733_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ11060.1"
                     /translation="MEPEHDFHAGAIETSLMMYWKPELVKNTIVMDEPEIARMMRTDQ
                     DWFEKVERKMEHKTHSNKFALYFLFL"
     gene            complement(4374..6764)
                     /locus_tag="PK733_10770"
     CDS             complement(4374..6764)
                     /locus_tag="PK733_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ11061.1"
                     /translation="MNIYSHIIFTALTINLFIMFYGFYYNHKTKSALNRLHICIILCV
                     SIWSLGSFFIYTAANRNTCWFWYKFSSVGQCFFSSFILHFSLVFTRKNLLLKKWWAYA
                     IIYIPSLLLLYGFLASNVIVNDFLITGSGHLELVIDYSFWYFYYAFLHLVYILSAIII
                     GLLWRAKLKYSSEKRQALNILISDILSLLTAIFLGLILPNLFGSTLLLLTPVAATFGI
                     IGTMYAAVKYEYIVLTPSLAAENILDTITDSVVLVNAELKILTVNNETCRLLKYTKNE
                     IIGNELKIFFGGDEIFNNINLKSMLQNGSVRNYEAGYVTKEGKTIPVIFSASEHKDKD
                     GNLIGIVVISRDITEQKKTEERLKYLAHHDYLTGLPNRVLFTDRLEQALFRASRYETM
                     AAVILLDIDHFKEINDTFGHTNGDAFLMEVAERLSSVIRQTDTIARLGGDEFIFIIND
                     LKYTSDIQKVVNKIHKAFYKSFIIGTRELHITASIGISLYPLNGSNADTLIKNADLAM
                     YHAKNQGRNNYQPFSHSLGVISTEKLLFENRLQKAFEQNEFIVHYQPIIDINTTKLVS
                     MEALVRWQHPEQGLIPPAEFIPSAEKTGLIVQIGEFVLRTACIQSRQWIEQGLSSIPI
                     SVNLSPYQFRQKNLVDRILCILAETGLESRFLTLEITETTAMFDVEKTSVILSQLCSH
                     GIKCILDDFGMGYSSLNYLSKFPIHAIKIDKTFIQDIINNSCNAAIISAILLMANAMK
                     LKVIIEGVENFEQLNYIRSLHWEPIEPQNRVAIQGYYFSKPLSANSFSQFLQKQKI"
     gene            7229..7642
                     /locus_tag="PK733_10775"
     CDS             7229..7642
                     /locus_tag="PK733_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11062.1"
                     /translation="MDKQIKFLPMSEGPINDYLSLMHKKGYQLINACQYFKGRKLHIN
                     NDNKCRDNYLILSFKKCSNPRIFFYKDFLAGGKMMEDIETELNDYTVKKQEEGYELFD
                     LVTNKKYCVSIDGLIHCSVANIGSFFMKEAKRDLL"
     gene            complement(7647..7722)
                     /locus_tag="PK733_10780"
     tRNA            complement(7647..7722)
                     /locus_tag="PK733_10780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7687..7689),aa:Ala,seq:cgc)
     gene            complement(7920..8912)
                     /locus_tag="PK733_10785"
     CDS             complement(7920..8912)
                     /locus_tag="PK733_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11063.1"
                     /translation="MIKVALIGAGRMGKVHFDAYKSIPDVKIVAAVDIEIQAAKERLN
                     DADVVFYDDLDCMLKKEKVDMVDICTPTYLHAEHAIKAMDKGVHVICEKPISLNVDDA
                     RKIVDAAHRNNVFFMVAQVIRFWPEYEYLKKVYDEKTYGKLYQILFTRVGEMTKSSWK
                     DWMRDISKSGMAPIDLHIHDVDFILHMFGKPNAVTSFAKQDGKNISWINTHYDYNDAI
                     IEAEGAWYQPKYSFSMSYRASFEDAILEYKSSKLTLYKSCGETTQIHLEDVKSTSQLN
                     VGSVNGYQYEIEYFVNCVRNNIPPKRIIPEESVMSLELVLKELESAREAKKVIV"
     gene            complement(9132..10865)
                     /locus_tag="PK733_10790"
     CDS             complement(9132..10865)
                     /locus_tag="PK733_10790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ11064.1"
                     /translation="MENLIFKDINVSDEVKKAIMDMGFEELTHIQDLSIPHILEGKDV
                     IGQAQTGTGKTCAFGIPAIELIDPESSTIQVLILSPTRELAIQISEELKSVSKYKKGI
                     GILPIYGGQPIDRQINALKKHPQIIIGTPGRVMDHMRRHTLKFSDLKMLVLDEADEML
                     NMGFREDIDTILERVPDERQTILFSATMPKEIMDLTNKYQKDPIYIQATRKELTVPGI
                     QQFYLEVSEASKLEVLSRLVDTNNIRLSLVFCNTKKRVDELTSALQSRGYGAEALHGD
                     MKQEHRDRVMAKFRKGHIDILVATDVAARGIDVDDIEAVFNYDIPEDVEYYVHRIGRT
                     GRAGKTGKAFSFVVGREIYKLRDIQRYTKSSVSLIKPPTIMDVEERKTVSLLEKLKET
                     INENNYKKYVPYIERFLQEIGDGSNNDNYITSLDAAAALLKMVSTQKGSQVNHVPDID
                     ERDINYESFKGDNIDGINIDENEEMVRFFINIGRKNGIQPRHIVESIASSTSLPGKLI
                     GAIDIYDAYTFVDVPGKYAAEVLSAMKNNTVKGKRVNIERSNKSRRKGSNRTGGSGSG
                     NRSGSRGRRRF"
     gene            11617..12600
                     /locus_tag="PK733_10795"
     CDS             11617..12600
                     /locus_tag="PK733_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11065.1"
                     /translation="MDLTEATLKKSTDQQKFNRAAARKDNFRKFIKNVSRGRYIYLLM
                     LPGVLYIFIFNYIPIYGLQIAFKEFSYSGSFTSGKWIGLVNFRDLVTEVEFWRAFKNT
                     IIISFMKIALGFPIPMILAILINEVFLKRYKRVLQTVLTFPHFLSWIVIGGIAFNLLA
                     NQGAINNLLSVLGLQRVNFLMNPGSFRYILVYSGVWKEAGWSSIIYLAAITNIEPELY
                     EAATVDGANRYQKMWYITWSGIKSMVVLMLIMSIGGMIQGNFLQVFFLYNPVVYSTGD
                     IIDTYVYRLTFQRGVDFSFTTAVGLFKGVVNLLLLLFANFVARRYGNRGVV"
     gene            12613..13479
                     /locus_tag="PK733_10800"
     CDS             12613..13479
                     /locus_tag="PK733_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11066.1"
                     /translation="MNKKAKFDAFDVVNLIILSIYGVLVFFPFYNTFIVSITTEFEYA
                     KTPFLLFPKKPDFLSYEVIFASKHVWKGFGVTIFVVLVGVIYQLFLTTTMAYGLGKKY
                     PGIKFISLAIVFTMYFGGGLIPFYLLMVNLKLVNSLFSMIIPAGIGIGYMVIMRDYFS
                     KIPKELEESALIEGANDMIVFYKIHLPLSTPLLVTISLYYGVDRWNDYYLAMLFIKDA
                     WKRPLQLILMEILTEYLKASENIPSTIQKRLYSEGMKMATIMFTTLPIMLVYPFLQKY
                     FVRGLMSGAVKA"
BASE COUNT         4462 a         2240 c         2459 g         4426 t
ORIGIN      
        1 tggagtggag gctatgggaa attagtaatt attgaccatg aaaatggtta taccacatat
       61 tatgcacata atgatactat aaatgtgaag aagggacaaa gggttgcaag gggtgataca
      121 ataggaactg caggctctac gggtaatgct acaggtccgc accttcattt cgaagtacga
      181 aagaacggag ttcctgttaa tccgcttaat tatgtcaagt gaataaggtc aagcgggtac
      241 atttataaaa tctgaagaaa tatttaccag gggctatcgt tacggtgctt agagccttca
      301 ttaatgcccc ttgccaatat tttcttcgaa taaatcaaat gcacccgggt caagaaaggc
      361 atatactacg ggagaataca tattacgcgt attatatttc ttcataaatt aaaaatgtac
      421 cctttttttc tacgggaagt tacacaaaaa catacagaag gagatatttg atatatgttc
      481 cgcaacactt tcaaagatat ttttaaaggt aaatatggta ttgacaactt aactcttact
      541 ttagtatttg cagcccttgt attttataac ataaaatatt tatgggtaat aggaattgtc
      601 ctttcaggat atgcaatatt cagaactttt tcgaaggaca ttaacaaacg ttatcaggaa
      661 ctgcaaaaat ttaatatgct ggtaaataag atatggcagt atattaacag aagtatacaa
      721 tggattcgct tccaatatgg tgtttgcagg acaagattgc agcaaagaaa gcaatatgtt
      781 ttttttaaat gccccaagtg taaaaaaacc cttcgacttc ccaaaaataa agggaagctg
      841 caggttacct gtccagtatg taaatatgaa tttttcaaga agacctagga atattccctc
      901 acgaatgctt cttccgataa tttcttcata ataggtgctc ataatttttt atacatgtgc
      961 ttgtaattat ttttatgttg acttttacta atttatcata tactattagt gtataagaca
     1021 ctaatacacg gagtgattac atgacagaat tcattactat aggtaaatca aaacagtttt
     1081 acgatttttt aaaaaacgaa attctcagtg gagaatataa agcgggagat aaattcccat
     1141 caatacggga tctggccgaa aagtatagtg tgagtaaaac cactgtaaat cttgttatat
     1201 caaaccttgt aaatgagaag ctcctatatg ttgaacaggg gaaaggtact tttgtttcag
     1261 ataaaaaacc ggattctaga agcagtaaaa agatgatagg agtaatgtta tttgatttca
     1321 ggtatgagaa taatgttgaa gctcctattt ttaacagtat ccaggaaaat ctcaaagacg
     1381 attattttct tataccatat aattcatatg ataacaatga aattttctat aaggggataa
     1441 aaggactaat ggagctggat gtagatggta tgatacttgt acccccaaca atggaagacc
     1501 atgatgctga ttacataaaa agtcttatta ccaaagacat tcctatagtt tttattaata
     1561 gacgaattcc tggaattaaa ggagatttct tttctatgga cttcgaaaaa ggggtttaca
     1621 aagcagcaaa atacattata gatagcggta agaaaagaat tgctatgttt aaaaacaaaa
     1681 gtcctagcct gcaggaacaa atgtataggg gatttgttgc tgctcataag gaggcgggat
     1741 tggagtttaa agacgagtat atgctggaat ggccatattg gtctaaaatg ggaatgggtg
     1801 gagatcctaa aatagctgaa atcagcctca aaaaggttat aaatgaaatc gatggactta
     1861 tagcaagtga ctattttata tataaaaaca gaaatgttat atatgagagc ggtaaggtta
     1921 ttccaagtga tatatctatt gttggtttaa atgatagtgc ttattcaaga tttatgaacc
     1981 caaaacttac agctatacca ttcctttccg aagaaattgg tgtgcaggct gtaaaaacaa
     2041 ttatggaaag gattgaggga aagaaaaaag ccgaagtaaa tatgtggctt gagcctgata
     2101 ttattatacg atcaagctag aagctaatat tactttgtta tgttgccaat agaaagcgta
     2161 tttccggaga gtaatttttg ctggtccgta aggaatacat cttcccgatg ctatcaaaaa
     2221 ttgtctcgga agacatcgcc ttccaaattg gaaagtcgta ctgatttgat taaaatataa
     2281 catgaaaggg gtctaaaaat gattaatgtt aagttcaagg gtattatgcc tgcaattatt
     2341 agtcctgtaa acgaggatgg ttctattagg gaaaaggagc ttcgccggct tataaattgg
     2401 caattggcgg caggttgttc agggttttat atttgcggtg caactggaga aggtactgta
     2461 atgaagccag aggcaagaaa agcaatggct gagatagctg ttgatgaagt aaagggacgt
     2521 ggttgtataa tagaccatat tggagctata gacttaaaaa cagccgctga tcttgccaga
     2581 catgcttctg atattggagt tgatgctata tcttctgttc ctcctttctt ttatacttac
     2641 ggagagaacg aaataatgaa ttactaccag gctgtttctg atgcatctga tgtacctata
     2701 ctcatgtatg catcacctct agcaggtaca aatataactg ccgaaatagt tgagaaaatg
     2761 ctgagcataa aaaatatgat aggtttaaaa tggaccagct ataattatta cgaaatgcgt
     2821 aaaatcaagg agctgaataa tagtgatata aatgtaatta acggacctga tgaaaccctt
     2881 ttgtgcggat tagctatggg tgctgatgga ggtattggtg ctacatataa tcccatgcca
     2941 aaaatttttg ttgatatata taacaatttt acagagggta aaattgatga agcgcagaag
     3001 gctcagttta aagcaaacaa agttatcagg attttactga aatatggtgt tttatgtggt
     3061 gtaaaagata tgctggatat gctcgggttt gatgttggtt attgtacata ccctttaaaa
     3121 aggtttacac cttcggagca ggaagctttc agagcagagc ttaaagctat caattttgta
     3181 gaaaaatatc tacataatta tgtagataat catgcagata attatgtaga taattaaata
     3241 tagccttata tagtcctgaa aagattatat tcatatttgc agaggagggt taatgaagtg
     3301 cgagatttat tttcaatgga aaacaaggct gtagtaatta caggtggggc cggatattta
     3361 ggttcagcga tggttgaggg cctgctggaa tttggtgcaa aggttatggt cgctgatatc
     3421 gctaaaaaga aacctgaaga aatagttaac aatggtaaaa gctatacaaa tctttatagc
     3481 atcgcctgtg atatttccag aactgaatct ataagggaat tatttaaaaa cgccagggaa
     3541 atttgtggag aaatagatgt tctcattaac tgtgcaacat atggagcagg aggatcaatt
     3601 gaaaccatga gtgatgaggc atggttaaaa ggtgtagacg ggagtctggg cactgtattc
     3661 aggtgcacaa gagaagtaat tccctatatg gctgaaaatg gtggaggttc tattgtcaat
     3721 atagcttcaa tgtatggaat ggtttctcct gacccgagaa tatacggaac aagcggacag
     3781 aacaatcctc ccaactatgg agccgccaaa gctggtgtta tacagctgac aagatactgt
     3841 gccggtcacc tggctgataa gaatatcagg gttaatagtg ttacaccagg cccatttcca
     3901 agcccggtga aacttccgcc ggaagagttt atgaataacc ttaaaaataa aaccatgctt
     3961 ggaagagtag ggaagccaag agatattgtt ggtgcagtaa ttctgctggc atcagatgca
     4021 tcaagtttta tgacaggatc caatattgtt gttgatggag gatggacagc atggtaataa
     4081 gggcttcgga agtgattctc gtttaaagga taggaatgag gaatatataa tagataggta
     4141 aacggaaggt agaaaaaaac aggtaattgg agtattttaa aatggagcct gagcatgatt
     4201 tccatgcagg tgctattgaa acctcactaa tgatgtactg gaaacctgaa cttgtgaaga
     4261 atacaatagt tatggatgaa ccggaaatag cacgaatgat gaggacagat caggactggt
     4321 ttgaaaaagt tgaaaggaaa atggaacata aaacgcacag caataaattc gcattatatt
     4381 ttctgttttt gtaaaaattg tgagaagcta ttagctgaga gcggtttgct gaaatagtac
     4441 ccttgtatgg caacacggtt ttggggctca ataggttccc aatgaagaga acgtatataa
     4501 tttagttgtt caaaattttc aactccttct ataattactt tcagcttcat ggcatttgcc
     4561 attagcaata tggcacttat aattgcagca ttgcatgagt tgttaattat atcctgtatg
     4621 aaagttttat ctatttttat agcgtggatt gggaatttac tgaggtaatt gagtgatgaa
     4681 tatcccattc caaaatcatc gaggatacac ttgataccat gtgaacaaag ttgactaaga
     4741 attacagatg tcttttccac atcgaacatt gctgtagtct cagtaatctc aagagtaagg
     4801 aaacgtgatt ctaaaccagt ttctgcaagt atacaaagaa ttctatctac caaatttttc
     4861 tgcctgaact gataagggga caagtttaca gaaatgggga ttgacgacaa accctgttca
     4921 atccactgcc tgctttgtat acacgccgta cgcagtacaa attctccgat ttgtacaata
     4981 agtccggttt tttcagctga tggtatgaat tctgcaggtg gtatcagacc ttgttcggga
     5041 tgctgccagc gtaccaatgc ttccatactt acaagtttag tggtatttat atctataatt
     5101 ggctgataat gaactataaa ctcgttctgt tcaaaagcct tttgaagtct gttctcaaat
     5161 agaagttttt cggtactaat tacaccaaga gagtgtgaga atggttgata gttgtttcta
     5221 ccctgatttt tggcatgata catggccagg tcagcatttt ttataagggt atccgcattg
     5281 ctcccattaa gtggatacaa gctgatacca atgcttgctg ttatatgtaa ttcacgggtt
     5341 ccaattatga agctcttata aaatgcttta tgtattttat taacgacttt ctgaatatcg
     5401 ctggtgtatt tcaaatcatt aattataaag ataaattcat caccacccaa acgtgcaatt
     5461 gtatcagttt ggcggattac tgagcttagc ctctctgcta cttccatcag gaaggcatca
     5521 ccattagtat gtccaaatgt atcattaatc tccttaaaat ggtctatatc aagaagtatt
     5581 actgcagcca tggtttcata acgagatgct ctgaacaaag cttgttcaag acggtcagta
     5641 aatagaaccc ggttagggag gcctgttaaa taatcgtggt gagcaaggta tttcaaacgt
     5701 tcctcagttt tcttctgctc tgtaatatct cttgagatca ctactatacc aattaaatta
     5761 ccatctttat ctttatgttc ggaagctgaa aaaataaccg gtatggtttt tccttctttt
     5821 gttacatatc ctgcttcata attccgtaca gaaccatttt gcagcatact ttttaaattt
     5881 atattgttga atatttcatc tccaccgaag aatattttca gttcattgcc tataatttcg
     5941 ttttttgtgt atttcaataa cctgcaagtt tcattattca ctgtaagtat ttttagttca
     6001 gcgttaacca gcacaacaga atcagttata gtatccagaa tattctcagc tgcaagggaa
     6061 ggcgtaagca caatatattc atattttacg gcagcataca ttgttccgat tattccaaag
     6121 gttgctgcaa ctggggttaa cagcagtaaa gtggaaccaa aaaggttagg aaggatcaaa
     6181 cctagaaaaa tggcggttaa caaggagagt atgtcagaga ttaaaatatt taaagcctgc
     6241 cttttttctg atgaatattt taattttgcc ctccaaagca gacctatgat aattgcgctt
     6301 aagatataga ctaaatgcag aaatgcgtag taaaaatacc aaaatgaata atcaataacc
     6361 agttccaaat gtcctgaacc tgttattagg aaatcattta caattacatt acttgctaag
     6421 aaaccatata ataaaagcag tgaaggtatg tatataatag cataagccca ccatttcttc
     6481 aggagcaggt tttttcttgt aaaaacaagg gaaaagtgca gaatgaagga tgaaaagaaa
     6541 cactggccga cagaagaaaa tttataccag aaccagcatg tatttctatt tgctgcagta
     6601 taaatgaaaa aggaaccaag agaccatatg gaaacacaaa gaattatgca aatatgtaac
     6661 cgatttaaag ccgatttggt cttgtgattg taataaaacc cataaaacat aataaaaaga
     6721 tttatagtaa gagctgtaaa tataatatga gaataaatat tcatactaat cacctcacca
     6781 taaacttaaa aagtactttc tcaacaaaac ccagcaattg atagaatatc tagtacttta
     6841 taccaatact tagttgaaat aaattttctt aaataaatat tcttgcaact aaacaataaa
     6901 aataaacttt tattttatgt ttcttgacat agttaattaa tgttttctaa aagcaaaaaa
     6961 ttactttatt cagcatatat ctacactatt tcatagtaac attcttacaa atattattca
     7021 agacttaaaa tgaattattt gtaaaactaa aatttgcaca tttattgttt atatccaaaa
     7081 agaatagcaa tattttcatc aaatgatatt ttgcaaaact tagagtagga attcatattt
     7141 tttcatgata tactgataat ataaaggttg atcaaaatac tttgcaagag gtaacattaa
     7201 aaaatgttaa agtgaggtgt aactatttat ggataagcag ataaaatttc tacccatgtc
     7261 tgaaggtcca attaatgatt acttgtcatt gatgcacaaa aaaggttacc aattaattaa
     7321 tgcatgtcag tatttcaaag gccgcaaact acatattaat aatgataaca aatgtagaga
     7381 taactatttg atattatctt ttaaaaaatg cagcaatcca cgcatattct tttacaaaga
     7441 ttttcttgcg ggtggaaaaa tgatggaaga tattgaaact gaattgaatg attatactgt
     7501 aaaaaagcag gaagaaggtt atgaattatt tgaccttgta acaaataaaa aatattgcgt
     7561 ttctattgat ggacttattc attgctctgt agctaatata gggagttttt ttatgaaaga
     7621 ggccaagaga gatttactat aagaattggt ggagcatagg ggatttgaac ccctgatctt
     7681 cgcgatgcga acgcgacgct ctcccagctg agccaatgcc ccataaatgt gcaataaatg
     7741 cataatatgt cataatatgt agttctttaa tttatcgcaa tactggtgca tttgtcaaat
     7801 atgaggaaat atttgataaa tgcacccgca gctcagctta tttatatgaa gctatacgcc
     7861 gcaataatac gcgataatat ataatgaaaa aattaaacaa tgatatatat ttattaattt
     7921 caaacaatca ctttcttagc ctctcttgca gattcaagct ccttcaacac caactcaagg
     7981 ctcattacag attcttcagg tatgatcctt tttgggggta tattgtttcg tacacaattt
     8041 acaaagtact ctatttcata ttgataacca ttaactgaac ctacatttaa ctggctggta
     8101 gattttacat cctctagatg aatttgggtt gtttctccgc agctcttgta aagtgtaagc
     8161 ttactgcttt tgtattccaa aatggcatct tcaaagcttg ctctataaga cattgaaaaa
     8221 gaatactttg gctgatacca ggccccttca gcttcaatta ttgcatcatt ataatcgtaa
     8281 tgggtattaa tccagcttat atttttacca tcctgctttg caaaacttgt tactgcattt
     8341 ggttttccaa acatatgtaa aataaagtct acatcgtgta tgtgaagatc tataggtgcc
     8401 attccgctct ttgatatatc tctcatccaa tctttccatg aagattttgt catttcaccc
     8461 acccgggtaa ataatatctg atagagtttc ccatatgttt tttcatcata tacttttttc
     8521 agatattcat attccggcca aaaccttata acctgagcaa ccataaaaaa cacattgttt
     8581 ctatgtgctg catctacaat tttcctggca tcatccacat tcaaagatat gggtttttca
     8641 caaatcacat gcacaccttt atccatagct tttatagcat gttccgcatg caaatatgta
     8701 ggggtgcaaa tatctaccat atcaaccttc tcttttttga gcatacaatc caaatcatca
     8761 tagaaaacta catcagcatc attaagacgt tctttagcag cttgtatctc aatatccact
     8821 gctgccacaa tttttacatc ggggatactt ttatatgcat caaaatgcac cttacccatc
     8881 ctgccggccc ctatcaatgc tacttttatc atattcagca cctcatttct aattagtact
     8941 atcaaataat tttcacagtt agtgccacta aataattttg gaaatagata taataatcca
     9001 atattaatcc taattttaat ctaaatagtt aaatggcgca atatgcatta agtataaccg
     9061 atgaataaat ttctctataa aaattggtga atataaacaa ataccctgtt aaaatgggat
     9121 ttattcacat atcaaaatct tcttctgccc ctgcttccac ttctatttcc acttccactt
     9181 ccaccagttc tattgctgcc cttcctgcgg cttttgtttg atctttctat atttactctt
     9241 ttgcctttta cagtattgtt tttcattgcc gataaaactt cagcagcata ttttccggga
     9301 acatcaacaa aagtatatgc atcataaata tctattgctc cgatgagctt accgggaaga
     9361 cttgttgaag atgcaatact ttcaacaata tgtcttggct ggataccatt ttttcttccg
     9421 atatttataa aaaatcgtac catttcttca ttttcatcta tatttatacc atctatattg
     9481 tcacctttaa aactctcata gttaatgtct ctttcatcaa tatcaggaac atggttaacc
     9541 tggctgcctt tctgggtaga taccattttt agcaaagctg cggctgcatc cagggaggta
     9601 atgtagttgt cgttattgct accatcacct atttcttgaa ggaatctctc aatataaggt
     9661 acatatttct tatagttgtt ttcgttaata gtttctttta gcttctccaa aaggctaact
     9721 gttttccttt cttccacatc cataatagtc ggtggcttta ttaacgaaac agatgatttt
     9781 gtgtatcttt gtatgtccct taatttatat atttcacgtc ccacaacaaa agaaaaggct
     9841 ttgcctgttt ttccggccct tcctgtcctt ccgattctat ggacataata ttccacatct
     9901 tcaggtatat cgtagttaaa aacagcttca atgtcgtcta catcaatccc gcgtgcagca
     9961 acatcggtag caactaaaat atctatatgg ccttttctga atttcgccat aactctatcc
    10021 cgatgttcct gcttcatatc accatgaaga gcttcggcac cataacctct ggattgcaaa
    10081 gcagaagtca gctcatcaac acgtttcttt gtattgcaaa aaaccagtga taatcttata
    10141 ttatttgtat caaccaacct tgatagcact tcaagcttgg aggcttcgct tacttcaaga
    10201 taaaactgct gaatcccggg gactgtaagc tctttcctgg ttgcttgaat atatattgga
    10261 tctttctggt atttgttggt caaatccatt atttctttag gcattgtagc agaaaacagt
    10321 attgtttgcc tttcgtcagg gactctttca agaattgtgt cgatatcttc cctgaaaccc
    10381 atattaagca tttcatcagc ttcatccaga accagcattt tcaggtctga aaactttaaa
    10441 gtatggcgtc tcatatggtc cataacacgg cctggtgtac ctattataat ttgcggatgc
    10501 ttttttaggg catttatttg cctgtctata ggctgtcctc cataaatagg gagtatcccg
    10561 atacctttct tgtatttaga tacacttttt aattcttcag agatttgtat ggcaagttca
    10621 cgggtaggag ataaaattag tacttgaatt gtggaagatt ccggatctat tagttctatt
    10681 gcaggaattc cgaatgcaca ggtttttcca gtaccggtct gagcctggcc gataacatcc
    10741 ttcccttcaa gtatatgagg aatagataag tcctgaatat gtgtcaattc ctcaaaaccc
    10801 atatccataa tagccttttt tacttcatct gaaacattta tgtctttaaa aatcaaattt
    10861 tccatttctt cattcctttt ttaatttatt attttgagtg catagcatgc ttgcacctta
    10921 attcagtaca atcggagcgc atactgatta tatttaaatg aacatcggta ataatattat
    10981 tattattaac agactaaatt taaataaaac atcattacca atatgtttgc aaatttttaa
    11041 tataatatag cattttcagt tatcggaaaa caaatatttt tggagtaaag ccttactatt
    11101 acaaatattc acttatactt gcgataggaa gacaatgaaa tccccaatca aatccagcat
    11161 gaaattattc acacggaatt caggtgcagg cttgcaacta tataacatta tgcctttaac
    11221 tggtgcccag gttaatgtac tttattgggt gtaaccctac accctccatc ccatctgcaa
    11281 atatttttgg gaatgctttt aaatagatta tatgtgattt attttataaa atcaatctat
    11341 tatgaaaaat ttttctttta tctattcatt tccttatgtc ttaaattttt tatatttatc
    11401 tattttaatt acctcccatt atatgacgat tctaatttca tatatttaac taaacaattt
    11461 tacaacataa taatgtgaaa tgcaaaaaaa aacagaaaat gcaaaaatga gtttagtgca
    11521 aaaactactg agtttattgc attttcccaa tcatggacaa ttgatataat atttttgtaa
    11581 catatataac gttaaagaat cggggcgaaa caagatatgg atcttaccga agcaacattg
    11641 aaaaaatcaa cagatcaaca gaaatttaat agagctgcag caagaaaaga taattttaga
    11701 aagtttataa aaaatgtatc aaggggcagg tatatatatc ttttgatgct tccgggcgta
    11761 ttatacattt ttatatttaa ttacatacct atttatggtt tacagattgc atttaaggaa
    11821 ttttcatatt caggatcatt taccagtgga aagtggatag gactggtaaa ttttagagat
    11881 cttgtgacag aagttgagtt ttggagagca tttaaaaata caattattat ttcttttatg
    11941 aagatagctt tgggctttcc aatccccatg atactagcaa tacttattaa tgaagtcttt
    12001 cttaaaaggt ataagcgcgt acttcaaaca gttctcacct tcccgcattt cctttcctgg
    12061 atagtaatag gaggtattgc attcaacctt ttagccaacc agggagctat aaataacctg
    12121 cttagtgttc ttggccttca gagagtgaat tttttaatga atccggggag cttccgttat
    12181 atccttgtat acagcggtgt gtggaaggaa gcagggtggt caagcatcat atatttagca
    12241 gcaataacaa atattgagcc agagctatat gaagcagcaa cagtagatgg agccaacagg
    12301 tatcagaaaa tgtggtacat aacctggagt ggtataaaga gtatggtagt acttatgtta
    12361 ataatgagca tcggaggcat gattcaaggc aattttctcc aggtattttt cttatataat
    12421 ccagtagtat attcaaccgg tgacattatt gatacttatg tatataggct tacattccag
    12481 agaggtgtag atttttcatt cacaacagca gtagggttat ttaaaggagt tgtaaacctg
    12541 cttctacttt tattcgctaa ttttgttgca cgaagatatg gaaacagggg agttgtataa
    12601 gggggtatga gtatgaataa aaaagcaaag tttgatgcgt ttgatgttgt aaatcttata
    12661 atattaagca tatatggagt tctggtgttc tttccatttt acaacacatt tatagtttca
    12721 attactactg aatttgagta tgctaaaact ccgttcttgc tttttccaaa gaaacctgat
    12781 tttttatcat atgaggttat atttgcttcc aaacatgtat ggaaaggatt tggagtaaca
    12841 attttcgtag tactggttgg cgttatttac caattatttc ttacgactac tatggcatac
    12901 ggacttggca aaaaatatcc gggcataaag tttataagtc ttgcaatagt ttttaccatg
    12961 tattttgggg gaggattgat accattttac ttgcttatgg taaatcttaa actggtgaac
    13021 agccttttca gtatgataat tcctgcaggt ataggtattg gatatatggt tattatgagg
    13081 gactattttt ccaagatacc taaggaactt gaagaatcag ctctaattga aggagccaat
    13141 gatatgatag ttttttacaa aatacatttg cctctttcta cacctcttct tgtgacaatt
    13201 tccttatatt atggggttga taggtggaat gattattatc ttgccatgtt atttataaaa
    13261 gatgcctgga agcgtccatt acagctcata cttatggaaa tattaacaga gtacttgaag
    13321 gcatctgaaa atataccgtc tacaattcag aaaaggcttt actctgaagg tatgaaaatg
    13381 gcgactatta tgtttaccac tttgcctata atgcttgttt acccatttct acagaagtat
    13441 tttgtaagag gacttatgtc aggagctgtg aaagcatgaa aaagagtttt aacacggctt
    13501 gcataaggtc ggttagaata aaataaaaaa aagaagataa ctcgtgatgg aaaaacagag
    13561 tagtcgaagt agttgaaagg agtgatt
//
LOCUS       DAOSDD010000111        13492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1784_length_13492_cov_4.989283, whole genome shotgun sequence.
ACCESSION   DAOSDD010000111 DAOSDD010000000
VERSION     DAOSDD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1784_length_13492_cov_4.989283"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PK733_10805"
     CDS             complement(<1..131)
                     /locus_tag="PK733_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11067.1"
                     /translation="MYNVNNLGKRIASYRKFMGITQEELADRLNITAQAVSKWENDL"
     gene            complement(330..1037)
                     /locus_tag="PK733_10810"
     CDS             complement(330..1037)
                     /locus_tag="PK733_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ11068.1"
                     /translation="MNKYLFWDFDGTLGGRIDGHRGLAWSMSLLEAIHEMHPKSRLTV
                     NDIVPHLKQGFPWHEPEKSHLHLNTSKLWWDNLEKVFIQAYENLGFSQEMTSELAQLA
                     RKRFLDLSRWELYEDTIPVLRKLQLHGWKHIIVSNHVPELDLIVSHLELDMYITDIIN
                     SAVVGFEKPNPRIYKAAFERALHAKDIWMIGDNINADVLGAEQAGIKAILVRSMDPRA
                     RYSFQDLYSVERFLQAN"
     gene            complement(1075..1509)
                     /locus_tag="PK733_10815"
     CDS             complement(1075..1509)
                     /locus_tag="PK733_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11069.1"
                     /translation="MSVFYYYISKKQLPSQVIEKAHSINYSLNEITRFVKDAKKGYCY
                     YNCDPVSVYISTTKAVNINEIINYRHTKENNYLLWLAEEFRRLGYFNFIRVWEDNTKC
                     DTGYYEAFFKKCKHTKISYIDFCWCLASNERGLEDDVLYKVR"
     gene            complement(1634..1792)
                     /locus_tag="PK733_10820"
     CDS             complement(1634..1792)
                     /locus_tag="PK733_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11070.1"
                     /translation="MAEIYDKMGYTQNYDLRGWNADVLFDNEAEDGKWTYISHVRLKK
                     TPDDRLYK"
     gene            complement(1957..2124)
                     /locus_tag="PK733_10825"
                     /pseudo
     CDS             complement(1957..2124)
                     /locus_tag="PK733_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2202..2954)
                     /locus_tag="PK733_10830"
     CDS             complement(2202..2954)
                     /locus_tag="PK733_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11071.1"
                     /translation="MINKFSNEPLYCQLKAIILRKIENNEYPEDSKIPSELELCEEYG
                     ISRPTVRQAISELTNNGYLYKVRGKGTFVAKSKKSIDIKDYSGFTPSILDGQNPENRI
                     IIHTIAVSDSEFEKLRSVFNISVSQYGKISFAEIIYHIKNSNDMYSLNKSYIPLNLFP
                     DIIDSVISAQPSHVIMKGKYPLIPAKSKSIMEIIYSDKEDTRFLEIQEGQPLIKIENM
                     LYSKNGQVVEYIVTKYRADKCRFIFENLEGRK"
     gene            complement(3206..4633)
                     /gene="purB"
                     /locus_tag="PK733_10835"
     CDS             complement(3206..4633)
                     /gene="purB"
                     /locus_tag="PK733_10835"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOJ11072.1"
                     /translation="MKNTYESPLNTRYASKEMLELFSPDKKFRTWRELWIALAETEKE
                     LGLDINDEQIYELKQFKDIINYETAEKKEKEVRHDVMAHVYAYGEQCPKARGIIHLGA
                     TSCYVGDNTDLIIMKQGLKILRDKLLTIIKKLSEFALKYKDMPTLGFTHFQPAQPVTV
                     GKRACLWLQDLLIDLEDLDYLLSNMKMLGSKGTTGTQASFLELFNGDYEKVRKLDKLV
                     AEKMGFDSVYPVSGQTYTRKQDSRIMNLLSSIAQSAYKFSNDMRLLQHLKEIEEPFEE
                     KQIGSSAMAYKRNPMRSERISSLARYVIVDALNPAITASTQWFERTLDDSANKRISIS
                     EGFLAVDAILNIYINIASNMVVYPKVIEKHLMEELPFMATEIILMEAVKKGKDRQEMH
                     ERIRVHSMAAASRVKIEGKPNDLIERISMDETFNISMEELQVLLKPENFVGCAPHQVE
                     DFINNHVLSVLSDMDITEVKIDLKV"
     gene            complement(4828..5763)
                     /locus_tag="PK733_10840"
     CDS             complement(4828..5763)
                     /locus_tag="PK733_10840"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOJ11073.1"
                     /translation="MIIVDAHSDTLTKIINENQSLYSNNCNIDFKRMVKRGKYIQFFA
                     AFIDPVYCQAYAMRRALELIDKLHEECNKHNDVVMLCCNYNDILSAFSQEKAVAMLSI
                     EGGEALQGSLSALRMFYKLGVRSICLTWNHRNDIADGVADSISGGGLTAFGRQVIIEM
                     NHLGMLIDLSHMSERGFWDVMTLSQQPVILSHSNAYKVCMHRRNLNDDQIAAVKKNGG
                     VIGINLYPDFLNQSGKAGIDDVIKHIEHIISIAGEDHIGIGADYDQTEPVFEGIDGIE
                     YTSGILEELLKRNYSSNTVRKIAGENFLRVIKEVL"
     gene            complement(5802..6206)
                     /locus_tag="PK733_10845"
     CDS             complement(5802..6206)
                     /locus_tag="PK733_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOJ11074.1"
                     /translation="MPSKKQEDNNNNSFINKRELGSFGENIAVDYLTKNNFIILERNY
                     RYGRFGEIDIIARENEYICFIEVKTRSSNLFGTPSEAVNIKKQNSIKTLAQIYLKQMD
                     MKNKNLRFDIVEILIQYNGLAVKCINLIRNAF"
     gene            complement(6252..7034)
                     /locus_tag="PK733_10850"
     CDS             complement(6252..7034)
                     /locus_tag="PK733_10850"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ11075.1"
                     /translation="MKKVSKCKITLKQVEESISELNPSEALKQLYILRENQELNLDRL
                     IEKYLKKEDSFKKEKVRFNNMCKYESEAYKKGFKYIAGIDEAGRGPLAGPVVAAVVIL
                     PENIFINNLKDSKKLSPKQRDELYEQIKNKALAFEIGMADEKSIDDINIFNATKKAME
                     QAVNSLKLKPDILLIDAMKLDNLNISQISITKGDNLSISIAAASIIAKVTRDRIIEEY
                     DNIYPQYGFAKHKGYGTKEHIEAIKKYGICPIHRISFVKNFI"
     gene            complement(7018..7893)
                     /gene="ylqF"
                     /locus_tag="PK733_10855"
     CDS             complement(7018..7893)
                     /gene="ylqF"
                     /locus_tag="PK733_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOJ11076.1"
                     /translation="MNIQWFPGHMAKTRKLITENLSMVDVVIELLDARIPLSSKNPEI
                     DVLIKNKPKIVAFNKSDLADKKISEAWGIWYNSNGCPIVFINSIQGSGINNLKSKLHD
                     MMKDKIEQEKQKGKLYRPIRTMILGIPNVGKSSLINKLAGKAKAETGDKPGVTRQKQW
                     VRLDRGIEMLDMPGILWPKFDDPEVGINLAITGAIKDDVFDIVEVASYLMEHLNLTYP
                     DLLKERYKLNIENMENIDGVRKGYELLEAAGRNRGCLIAGGKVDFNRIAAIILDEFRG
                     GKIGKITLERPEKSE"
     gene            complement(7994..8602)
                     /gene="lepB"
                     /locus_tag="PK733_10860"
     CDS             complement(7994..8602)
                     /gene="lepB"
                     /locus_tag="PK733_10860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ11077.1"
                     /translation="MEKAEKKKNSFIEFLKWIETFVIAIFIALVIRGFIMEPVIVHGI
                     SMEDTLVNGQRLIIYKLGYFLHPPERGDIIVLQYQKGSMINIPFLNKFAFWDKVLPSP
                     SEVDYIKRVIGIPGDKLDVKEDGYIYINDVKQEEPYIKGKTYKQIMDFPTKVPPNTVF
                     VMGDNRQNSRDSRMIGFIEFDRIKGKAVLRIWPFKDWGFINR"
     gene            complement(8719..9108)
                     /gene="rplS"
                     /locus_tag="PK733_10865"
     CDS             complement(8719..9108)
                     /gene="rplS"
                     /locus_tag="PK733_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOJ11078.1"
                     /translation="MDYIKAIEEEQIRTDLQEVEVGDFVKVHLKIKEGNRERIQVFEG
                     TVIAKKGGGIKETITVRKISYGIGVERILPIHSPKISKIEVVRKGKVRRAKLYYLRDR
                     VGKAAKVKQKLQSKASLNADSTVTDEE"
     gene            complement(9281..10015)
                     /gene="trmD"
                     /locus_tag="PK733_10870"
     CDS             complement(9281..10015)
                     /gene="trmD"
                     /locus_tag="PK733_10870"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOJ11079.1"
                     /translation="MTFEVLTLFPEMFQAVLGESIIGRAQEKGLIRVNTVNIRDYSLD
                     KHRKTDDYPYGGGPGMVMMAQPIYDAYLSTMENIDYKPYVVYMSPQGKVLNQEMVKKL
                     INLKHIVILCGHYEGVDERVINKIVDEEISIGDYVLTGGELPAMVLIDCISRCIPGVL
                     ASGEAYTDESHYNGLLEYPQYTRPYEYLDMKVPDILISGHHANISIWRKQQALLRTYL
                     KRPDLYEKLSLNDEDLNLLKEVLEDS"
     gene            complement(10005..10583)
                     /gene="rimM"
                     /locus_tag="PK733_10875"
     CDS             complement(10005..10583)
                     /gene="rimM"
                     /locus_tag="PK733_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516314.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOJ11080.1"
                     /translation="MELKMQPFLEIGKIINTYGIKGELKVIPLTDSPQRYYDLEWAFV
                     GDDNVLEKYNIEKVRILKDLVIVKFKELNDINQAEKMKGQFIKVNRENAVKLPENTFF
                     ICDLINCEVLNYIDEKKLGTLKDIIQTGSNDVYIVQEESSEGSQGSQGSQGSEGFREI
                     LIPALKSVVKKVSVEEKKMWVSLPKGLVDHDF"
     gene            complement(10844..11074)
                     /locus_tag="PK733_10880"
     CDS             complement(10844..11074)
                     /locus_tag="PK733_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOJ11081.1"
                     /translation="MKELLESIVRALVDNPEEISVNEIEGEKSLILELKVASDDMGKV
                     IGKQGRIAKAIRTVMKAAAVKENKRVVVEIIQ"
     gene            complement(11146..11391)
                     /gene="rpsP"
                     /locus_tag="PK733_10885"
     CDS             complement(11146..11391)
                     /gene="rpsP"
                     /locus_tag="PK733_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOJ11082.1"
                     /translation="MAVKIRLKRVGAKKSPHYRVVVADSRYPRDGRFIEEIGYYNPAS
                     EPSVVNVDKEKAIQWIKNGAQPTDTVRALLKRLEIIN"
     gene            complement(11515..12858)
                     /gene="ffh"
                     /locus_tag="PK733_10890"
     CDS             complement(11515..12858)
                     /gene="ffh"
                     /locus_tag="PK733_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOJ11083.1"
                     /translation="MALFEGLSSKLQETINKMRGKGRVTEKDVKDMMREIRLALLEAD
                     VNYKVVKDFVNKISERAVGNEVLESLTPGQQVIKIVHEELIELLGNVPSKITFTSTPP
                     TVYMLVGLQGAGKTTFAGKLGNMLRKQGKSPLLVACDVYRPAAIKQLEVVGGQLNIPV
                     FSMEGVKNPVKIASEAIKHAKSKQYDIVIIDTAGRLHINDELMDELLNIKNAVKPQEI
                     LLTVDAMTGQDAVNVADSFNQKIGIDGVILTKLDGDTRGGAALSVKSITGKPIKFAGI
                     GEKLNDIEPFYPDRMASRILGMGDVLSLIEKAQEAFDEKKALEMEKKLRMQQFTLDDF
                     LDQMQELKKMGPLDQILGMIPGMNPKVKNNIKIDESKFTRMEAIINSMTRKERTNPSI
                     VNGSRRKRIAAGSGTAVQDVNKLLKDFEEMKKLIKTFDGMGKRGKKALGNFKMPF"
     gene            complement(12957..13313)
                     /locus_tag="PK733_10895"
     CDS             complement(12957..13313)
                     /locus_tag="PK733_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HOJ11084.1"
                     /translation="MEKIYEIGLLLDYYGQLLTKRQYEILDLYYNNDYSLGEISEELG
                     ISRQGVYDNLRRGMALLNKYESSLGLLERRFKQKDKVKFLLELVEEIDATVMNNNDKE
                     ILTKIRETVKSLFDDI"
BASE COUNT         3955 a         2744 c         1843 g         4950 t
ORIGIN      
        1 cataaatcgt tttcccattt ggatactgct tgtgcagtaa tatttaatct gtcagcaagt
       61 tcctcctgag ttatgcccat aaatttccta tatgatgcaa tccttttgcc taaattatta
      121 acgttataca tacaaaacat ctccttattt catattgtgc atgcatgcct tgtattttta
      181 gtatatatat tatattatac cgttacaaca tattaatagt ttattaaaag acaaaactat
      241 actgtccaaa ttcaaccatt ggttgactca accgcaggtt taaaatactt gttttctaaa
      301 gccatattca aaacatcctt ttatgaatct taatttgctt gcaaaaaacg ttctacagaa
      361 tataaatcct gaaagctgta tcttgctctc ggatccatac tgcgtactaa tattgctttt
      421 atacctgctt gttctgcccc caaaacatca gcattaatat tatccccaat catccagata
      481 tcttttgcat gaagtgccct ttcaaaagct gctttatata tacgggggtt gggtttttcg
      541 aatccaacta cagcagaatt aattatatca gtaatataca tatctaattc caaatgactc
      601 acgattaaat ctagttctgg aacatgattg gagacaataa tgtgcttcca cccatgcaat
      661 tgtaatttcc ttaagacggg aattgtatct tcatacaatt cccagcgact taaatctaga
      721 aaccttttcc gagctaattg ggctaattca gaagtcatct cttgagaaaa gcccaaattt
      781 tcgtatgctt gaataaaaac cttctcaaga ttatcccacc ataattttga agtatttaaa
      841 tgtaaatgac ttttttctgg ttcatgccat ggaaaaccct gttttaggtg tggaactata
      901 tcattaacgg tcaatctgga ttttggatgc atctcgtgta ttgcttccaa caaagacata
      961 ctccatgcca gcccacgatg gccatcaatt ctacctccga gagttccatc aaaatcccaa
     1021 aacaaatact tattcatttt acttcttcct ccaattttaa ttatatacac agcattatcg
     1081 cactttatat aaaacatcat cttctaagcc gcgttcatta cttgcgagac accaacaaaa
     1141 atctatgtag cttatttttg tatgtttgca tttcttaaaa aaggcttcat aatagcctgt
     1201 atcgcatttc gtattatcct cccataccct tatgaaatta aagtatccaa ggcgtctaaa
     1261 ctcttctgct aaccacaaaa ggtaattatt ttccttagta tgtctatagt taataatttc
     1321 attaatgttt actgccttag tagttgaaat atagacacta actgggtcac aattataata
     1381 acaataacct tttttcgcgt ctttaacaaa tctagttatt tcatttaaac tataattaat
     1441 ggagtgagct ttctcaataa cctgagaggg taattgcttt tttgaaatat agtagtaaaa
     1501 tacactcata aaaatcacct tcacataatt attctaaaca aattctctca tcgtccgaag
     1561 gattgagtga tgtccgtata taaggatata taaaaaatgt gtactgaagg ttatgctcat
     1621 ttagcgaagt tgattatttg tataatctgt cgtcaggagt tttcttaagc cgcacatgcg
     1681 aaatatatgt ccatttccca tcttcggctt cattgtcaaa gagtacatcc gcattccaac
     1741 cgcgcaaatc ataattttga gtgtatccca ttttgtcgta tatctctgcc atcaagtaac
     1801 cttcaccgtg tataccatac atgtgcgtca taacttattt tcgatacatt atagcaaaac
     1861 ggaaagaggg aattgacggg attttcctcc catttaccgc ctttcggctc aaattacctg
     1921 taatagaccc acctgaagcc ttcaggaatc acgtaaacgt tccgggaatg tatcaagagt
     1981 ttcgattgcg tcagctattc tgttgtactg tcccatggcc gtttccggtg cctgtagctg
     2041 ctctgcgata taattataaa tctcctccat atcggaaagt gccttgtccg ttgtaagctt
     2101 tcctgaacgt tttgtatttg tcaacctaag attagatcaa aagatcattt aattttaagt
     2161 cactttataa gaatagaatt aaccaaaaca tgtaaaactc tttactttct accttcaagg
     2221 ttctcgaaaa taaacctgca cttatctgct ctgtactttg taacaatata ttcaactact
     2281 tgtccatttt tagagtagag catattctct atttttatta atggctgtcc ttcctgaatc
     2341 tcaagaaaac gtgtatcttc cttatcacta tatattattt ccataatgct ttttgatttg
     2401 gcaggaatta aagggtattt tcccttcatt attacatggg atggctgtgc agatataacg
     2461 ctatcgataa tatctggaaa taaatttaaa ggtatatagg atttattaag agagtacata
     2521 tcattactat tttttatatg gtatattatt tcagcaaaac ttattttgcc gtattgtgaa
     2581 actgaaatat tgaatacact tcgcaatttt tcaaattcag agtcagaaac agctattgta
     2641 tgaataataa ttctgttctc cggattttgt ccatcaagta tagaaggtgt aaatccagaa
     2701 taatctttaa tatcaatact tttttttgat tttgctacga aggttccctt acctctaacc
     2761 ttatataaat aaccattgtt agtaagctca gaaattgctt gacgtacggt aggccggcta
     2821 ataccatatt cctcgcacaa ttctaattct gaaggtattt tagaatcttc aggatattcg
     2881 ttgttttcaa tctttcttaa tataattgcc ttcagctgac agtataaagg ttcattacta
     2941 aatttgttta tcaatggttt agccacctcc aatattacta caacacagca aactgcatta
     3001 atataatttc tttaaccata gataacgcac gaaagaaact acattattta ggttttgcgt
     3061 gcttatgtta ccttagtgaa taaaggtgca cacttgcacc taaatgggag gtacgcaaaa
     3121 agctaatctg ttccttgtag tatcattttt acgttatatt atgaagtata taaattatat
     3181 aattttcata ttttatatgc taattttata ctttcaaatc aatctttact tcagtaatgt
     3241 ccatatctga caacactgat aatacatgat tgttaataaa atcttcaact tgatgtggag
     3301 cgcatcccac aaaattttcg ggtttcaaaa gtacctgaag ttcttccatg cttatgttga
     3361 aggtttcatc cattgatatt ctttcaatca aatcatttgg cttgccttca attttaaccc
     3421 tggatgcagc tgccattgaa tgtaccctta tcctttcatg catttcctgc cggtcttttc
     3481 ccttctttac agcctccatc aaaatgattt ctgttgccat aaatggcaat tcctccatga
     3541 gatgcttttc tattacctta ggatatacaa ccatattaga agcaatattt atatatatat
     3601 ttagtatggc atccacagct aaaaagcctt cggatatgct tattcttttg tttgcggaat
     3661 catcaagagt tctttcaaac cactgggtag atgcagtaat cgcagggttt aaggcatcca
     3721 caataacata ccttgccagt gacgaaattc tttcacttct cataggattc cgtttatatg
     3781 ccattgctga ggaacctatc tgtttttctt cgaaaggttc ttcaatttcc ttcaaatgtt
     3841 gcagcaatct catgtcatta ctgaatttat atgcactttg ggctatactg ctaagcaaat
     3901 tcataatcct gctgtcctgt ttcctcgtat aagtttgtcc ggatacagga tatacactat
     3961 caaaacccat tttctcagca acaagcttgt ccagtttcct taccttctcg taatcaccat
     4021 tgaaaagctc aagaaaagat gcttgagttc ccgttgtacc ttttgatcca agcattttca
     4081 tgtttgaaag taaataatca agatcctcca ggtctattaa aagatcctgc agccacaaac
     4141 atgctctttt tccaactgtt acgggttgag caggctgaaa atgcgtaaat cctaatgtag
     4201 gcatatcttt atattttagt gcaaactcag ataacttttt tataatagtt aaaagcttgt
     4261 cccttaatat tttcagacct tgcttcatta ttataagatc agtattatct cctacgtagc
     4321 atgatgtggc acctaaatga ataatacccc ttgctttggg acattgttcc ccgtatgcat
     4381 aaacatgtgc cattacatca tgtctgacct ctttttcttt cttttcggcc gtttcataat
     4441 ttattatatc tttaaattgt tttaattcgt atatttgttc atcattaata tccagtccca
     4501 attccttttc tgtctctgca agagcaatcc acaattctct ccaggttcta aactttttat
     4561 cgggtgaaaa caattccagc atttctttac ttgcgtacct ggtatttagg ggactttcat
     4621 atgtgttttt cattagtctt tcctccatta gaataatgcg tattaagtat tctataaatt
     4681 caattataca tcgtttatat aaaactaagc aagtataatt aagttaactg gatgcagagc
     4741 gcatttttaa tttatgaaaa aatataatta gcgtctccag atacacctat aatgtctaga
     4801 taatcgaata tgtgaagtag gactaaccta caatacttcc ttaattactc ttaaaaagtt
     4861 ttcgccagct atctttctta cagtattgct tgaatagttc cttttcagta attcctccaa
     4921 aataccactt gtatattcaa tgccatcaat tccttcaaat acgggctcag tctgatcata
     4981 atccgcacct attcctatat ggtcttcacc tgcaatgcta ataatatgct caatatgctt
     5041 tattacatca tcaataccag ctttacccga ttggttcaaa aaatccggat ataaattgat
     5101 accaataact ccgccgttct tctttaccgc agcaatttga tcatcattaa ggtttcttcg
     5161 gtgcatgcat actttataag cattagaatg agataaaata acaggctgtt gtgataatgt
     5221 cattacatcc caaaaacctc tttctgacat atgagataga tcaataagca tgccaaggtg
     5281 gttcatttct attattactt gacggccaaa agctgtcaat ccacctcctg aaattgagtc
     5341 ggcaactcca tctgcaatat cattcctatg attccatgtt aagcaaatac ttcttacccc
     5401 aagtttataa aacattctca atgctgacag gcttccctgt aaagcttctc caccttcgat
     5461 cgacagcatg gccacagcct tttcctgtga aaatgcggat aaaatatcgt tgtaattaca
     5521 gcataacata acgacatcat tatgtttatt acattcttca tggagtttat ctataagttc
     5581 aagtgctctt ctcatagcgt atgcctgaca atatacagga tctatgaaag cagcaaaaaa
     5641 ctgtatatat ttgccccttt ttaccatacg tttaaagtca atattgcagt tgtttgaata
     5701 gagactctga ttctcattta tgatttttgt cagtgtgtcg gaatgagcat caacaataat
     5761 catataaaaa cctccgctaa agtcatctcc acatagtatt attagaatgc atttcttatc
     5821 aggtttatac acttaaccgc taaaccattg tattgaataa gtatttctac gatatcaaat
     5881 ctcaaatttt tattcttcat gtccatttgc tttaagtata tttgggccag tgtttttatg
     5941 ctattttgct ttttaatatt tactgcttca gaaggtgtgc cgaatagatt gctgctcctt
     6001 gtttttactt ctataaagca tatatattca ttttccctgg caataatgtc gatttctcca
     6061 aatctgccat acctatagtt acgctccaat ataataaaat tgtttttagt taaataatca
     6121 actgcaatgt tttctccaaa actgcctaat tctcttttat ttataaaact attattatta
     6181 ttatcttctt gtttctttga aggcaaagtt ttaccccttt ctgtaagata cacattcaga
     6241 aatctatgct gctaaatgaa gtttttaaca aagcttattc tatgtatcgg acagattcca
     6301 tatttcttta tagcttctat atgttctttt gtcccataac ccttatgctt tgcaaaacca
     6361 tattgggggt atatattgtc atattcttct attattctgt ctcttgtaac tttggctatt
     6421 attgaggctg ctgctattga aatgcttaag ttgtcacctt tggtaataga tatttgagat
     6481 atattcaaat tatcaagttt catagcatct atcaataata tgtcgggttt gagtttcaaa
     6541 gaatttacag cttgctccat tgctttcttt gttgcattaa agatatttat atcgtctata
     6601 gatttttcat cagccatacc tatctcaaaa gcaagtgcct tgtttttaat ttgctcataa
     6661 agctcatcac gttgttttgg acttaatttc tttgaatcct ttagattatt aataaaaata
     6721 ttttccggca gtatgacaac tgcagctact accggacctg caagagggcc acgccctgct
     6781 tcatctattc cggcaatgta tttgaagcct tttttataag cttcagattc atacttgcac
     6841 atattattaa atcttacctt ttctttttta aaagaatctt ctttttttag gtatttttca
     6901 ataagtctat ccaaattaag ttcttgattt tcacgtaata tataaagctg tttaagagct
     6961 tctgaagggt ttagttcaga aatactttct tcaacttgct taagcgtaat tttacattta
     7021 ctcacttttt tcaggccttt ccaaggttat tttgccgatt tttcctcctc tgaattcgtc
     7081 taggataatg gcagcaattc tattaaaatc aactttgcca cctgcaataa ggcatccacg
     7141 gtttctcccg gcagcttcga gaagttcata tccttttctg acaccatcta tattttccat
     7201 attttctata tttaatttgt atctttcttt gaggagatct ggatatgtta aattcaaatg
     7261 ctccatcaaa tacgaagcaa cttcaacaat atcaaataca tcatctttta tagctccggt
     7321 tatagccaaa ttaataccta cctctggatc atcaaactta ggccataata taccgggcat
     7381 atcaagcatt tcaattccac ggtcaagcct gacccactgc ttttggcgcg ttactccggg
     7441 cttgtcgccg gtttcggctt ttgcttttcc tgcaagttta tttataagag aagacttgcc
     7501 tacattgggt attcccaata tcatagtcct tattggtcta taaagcttgc ctttctgttt
     7561 ttcctgttca attttatcct tcatcatatc atgtagcttt gatttaagat tgtttatacc
     7621 gctgccttga attgaattta tgaaaacaat aggacatcca tttgaattat accaaatacc
     7681 ccaggcctca gatatttttt tatcagccag atctgattta ttaaaagcaa ctattttggg
     7741 cttgtttttt atcaatacat ctatttcagg atttttactg cttaatggaa ttcttgcatc
     7801 aagcagttca ataactacat ccaccatgct tagattttct gttattaatt tcctggtctt
     7861 tgccatatga cctggaaacc attgtatgtt cattgtttcc tccttagtta aatatatata
     7921 gcttgttttg gttaaatata tataacttgt taattaatta gtcaattaat aactatcatt
     7981 gatgcttatt attttacctg ttaataaaac cccaatcttt aaaaggccat atccttagta
     8041 cagcttttcc ctttatcctg tcaaattcta taaacccaat cattctgcta tctctgctgt
     8101 tttgtctgtt atctcccata acaaaaacag tatttggtgg aacttttgta gggaaatcca
     8161 ttatttgctt gtatgtttta ccttttatat aaggctcctc ctgcttaaca tcgttaatat
     8221 atatatagcc atcttcctta acatcaagtt tatcccctgg tataccaatt actcttttta
     8281 tgtaatctac ttctgaaggg gaaggaagta ctttatccca aaaagcaaat ttgttaagga
     8341 agggaatatt tatcatagac cccttttggt attgaagaac aataatatcc ccgcgctcag
     8401 gaggatgaag aaagtatcca agcttataaa taataagtct ttgaccatta acaagagtgt
     8461 cttccattga aataccatgt actataacag gctccataat aaatcctctt attaccaaag
     8521 ctataaatat tgcaataaca aatgtttcaa tccatttaag aaactctatg aaactatttt
     8581 ttttcttttc tgctttttcc ataataatat tacccgcttt atatttaaat atacttaaca
     8641 atgttgaact tgagtgaaag ttataacata aaagggacgc ttgtcccttt tatgtatact
     8701 ttattattca ttttggttct attcttcatc ggtaactgta ctgtctgcat tcaatgaagc
     8761 tttgctctga agtttttgtt tgactttagc agctttacca actctatcac gaagataata
     8821 aagtttagct cttctgacct taccttttcg tacaacttca atcttgctga ttttgggaga
     8881 atgaataggt agaattctct ctacaccaat accataagat atctttctta cagttattgt
     8941 ttcctttatt cctccgcctt ttttggcaat tactgtccct tcaaaaacct gtattctttc
     9001 tctattacct tctttgattt ttaaatgaac ttttacaaag tcaccaactt caacttcttg
     9061 aagatctgtt cttatttgct cttcttctat tgctttaata taatccacta tcttttcctc
     9121 cttccttcca ggacgttcat gcaaaaaaca ctaaataatt tacagaggac cgcccgtaat
     9181 tcacacgata agtattatat cacaagtgat tttctttgta aactgaattt tttccaaata
     9241 ttttgtaaat atttggaaat atttagtaat tataaatcct ttatgaatcc tccaaaactt
     9301 ccttaagcag attcaaatct tcatcattta aagataactt ctcatataga tccggacgtt
     9361 tcaaatatgt tctcagcaat gcctgctgtt tcctccatat gcttatattg gcatgatgcc
     9421 ctgaaataag tatatcgggt actttcatgt ccaaatactc atatggtctt gtatactgag
     9481 gatattccaa taaaccgtta taatgggatt catctgtata tgcttctcct gaagctaata
     9541 cacccggaat acatcggctt atacaatcta taagtaccat ggccggcaat tctccgccag
     9601 ttagtacata atctccaata gatatctctt catctactat tttgttaata actctttcgt
     9661 caacaccttc gtagtgacca caaagtataa caatatgctt caaatttata agcttcttta
     9721 ccatttcttg atttaaaact ttaccctgag ggctcatata aactacatat ggcttatagt
     9781 caatattttc cattgttgaa agatatgcat catatattgg ctgtgccatc ataaccatac
     9841 cggggcctcc tccataagga tagtcatctg ttttcctgtg cttgtccaga gaataatccc
     9901 tgatatttac cgtatttact cttattaacc ccttttcctg agctcttccg ataatacttt
     9961 cacccaaaac tgcctgaaac atttcgggaa agagggttaa aacttcaaaa gtcatgatcc
    10021 accaaccctt tgggcaaaga tacccacatt ttcttttctt ctacagaaac cttttttact
    10081 acacttttca aggcgggaat aagtatttct ctgaagcctt ctgagccttg tgagccttgt
    10141 gagccttgtg agccttctga gctttcttcc tgaactatat aaacgtcgtt gcttcctgtt
    10201 tgaataatat ctttgagagt accaagtttc ttttcatcta tataattaag gacttcgcag
    10261 ttaattaaat cacaaataaa aaatgtattt tcgggaagtt tcactgcgtt ttccctattt
    10321 accttaataa attgtccttt catcttttca gcttgattta tatcatttaa ttctttaaat
    10381 ttaactatta caagatcctt taaaatcctg accttttcta tattatactt ttcaagcaca
    10441 ttatcatcgc ctacaaatgc ccattccaag tcataatatc tttgtggaga atcggttaag
    10501 ggtattacct taagttcccc ttttatacca taggtattaa taattttgcc aatttccaaa
    10561 aaaggttgca ttttaagctc cattcctgct gcgtgcgaaa tgaattcgcc cttgcaggaa
    10621 caaaactttt ttatgaaaaa gtttttatgg gcttaaactc attttagccg cgcgtgcaat
    10681 ttattttttt agaaatactt atgaaaaaaa tatgcacatg cgcattagtt tttcagaaat
    10741 ttatcaattt ccttcaaact aagctccatt cctgctgcgt gcgaaatgaa ttcgtctttc
    10801 ttcaatagtt atccccccaa aagagggggg atattacggt aagttactgg ataatttcta
    10861 caactactct cttattctcc ttaactgccg ctgccttcat aaccgtcctt attgccttcg
    10921 caattctgcc ttgttttcct attaccttcc ccatatcatc agaggcaacc tttagctcta
    10981 atattaatga tttttcacct tcaatttcat taaccgaaat ttcctcggga ttatctacta
    11041 acgctctgac aatagattcg agcagctctt tcattctcaa tacctcccgc atttactata
    11101 attaataatt ataaatagta ctatttatgt taccgccaaa ttcaactagt taataatttc
    11161 aagtctcttt aaaagggctc taactgtatc ggtaggttga gcaccatttt taatccattg
    11221 aatggctttt tctttatcta cattaaccac tgaaggttct gatgcagggt tataataacc
    11281 aatttcttct ataaatctgc catcacgggg atatcttgaa tctgcaacaa caaccctgta
    11341 atggggactt ttctttgcac ctactctttt aagtctgatt ttaactgcca ttaaattcac
    11401 ctcctaattt gattattaat tgagatatag tttatataag tgatatatcc acaaatacaa
    11461 atgtatatct gatatattac acttctgtcg gttttgcgac atgaagtttg ctaactaaaa
    11521 aggcatttta aagtttccaa gggctttttt ccctcttttc cccataccat caaaagtttt
    11581 tatcaatttc ttcatttcct caaaatcctt taaaagcttg ttaacatctt gaacggcagt
    11641 accgctgcct gcagctattc ttttgcgtct gctgccatta acaattgatg ggttggtcct
    11701 ttctttccta gtcattgaat ttattattgc ttccatcctt gtaaatttac tctcgtcaat
    11761 tttgatatta ttcttcacct ttggattcat gcctggaatc atacccagaa tttggtccaa
    11821 tggtcccatt ttcttgagtt cctgcatctg gtcaagaaaa tcatccagcg taaattgctg
    11881 catacgaagt ttcttttcca tttcaagagc ttttttttca tcaaaagcct cctgtgcctt
    11941 ttcaataagg ctaagcacat cacccatacc aagaattctt gatgccatac gatcaggata
    12001 aaaaggttct atatcattga gcttttctcc aatacctgca aatttaatag gttttcctgt
    12061 tatagatttt actgataacg cagcaccgcc tcttgtatca ccatcaagtt ttgttagaat
    12121 aacaccatct ataccaattt tctgattgaa gctatctgct acatttacag catcctgccc
    12181 tgtcattgca tcaacagtaa gaagtatttc ctgaggtttt actgcatttt ttatatttaa
    12241 cagttcatcc atcaattcgt catttatatg caatctacca gctgtatcta tgattacgat
    12301 atcatactgt ttgctctttg catgtttaat agcctctgag gctattttca caggattttt
    12361 taccccctcc attgaaaata ctggtatatt caactgacct ccgacgactt ccagctgttt
    12421 aatagcagct ggcctgtaaa catcacaagc caccagtaaa ggtgatttcc cctgcttgcg
    12481 aagcatattg cccaatttac ctgcaaaggt agttttacct gcaccctgaa gtccaaccag
    12541 catatatact gttggaggag ttgatgtaaa agttatttta ctaggtacat tacctaaaag
    12601 ctcaatcaac tcttcgtgca caatttttat tacctgctga ccaggagtaa ggctttccaa
    12661 tacttcattt cccactgctc tttccgatat tttatttacg aagtctttta caactttata
    12721 attaacgtca gcttcaagta aagcaagtct gatttctctc atcatatctt ttacatcttt
    12781 ttctgtaact cttcctttac cgcgcatttt atttatggtt tcctgcagtt tggaagacaa
    12841 accttcaaat aaagccattt caaaactcct ttccatatca tcagataaat acattaatat
    12901 aattaacaaa tgtaattaac aactaaatgc ataaaaatag ttaacatcta aatactttat
    12961 atgtcatcaa ataagctctt tactgtttcc ctgattttgg ttaatatttc cttgtcatta
    13021 ttattcataa cagttgcatc tatttcctca acaagctcca ataaaaattt gactttatcc
    13081 ttttgtttaa agcgtctttc taaaaggccc agtgatgatt catatttatt taacagagcc
    13141 attcctcttc ttaaattatc ataaactccc tgcctgctta ttcccaattc ttcggaaatt
    13201 tcccccaaag aataatcatt attataatat aaatcaagta tttcgtactg gcgtttggtc
    13261 aacaattgcc cataatagtc aagcaataag cctatttcat atattttttc catgtaattc
    13321 accaatgtaa agtatttata ctttacacct tattttaaag tgttttatgt gatttgtcaa
    13381 tataaatatt tggcgctcgc aattttatta ttcattttat tcttcgtaga atagcgcctc
    13441 cctaattctg cagtaaaaat ttaacaaatc attaatcaaa cccttgatac aa
//
LOCUS       DAOSDD010000112        13488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1787_length_13488_cov_6.171965, whole genome shotgun sequence.
ACCESSION   DAOSDD010000112 DAOSDD010000000
VERSION     DAOSDD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1787_length_13488_cov_6.171965"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK733_10900"
     CDS             <1..357
                     /locus_tag="PK733_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11085.1"
                     /translation="SLLARNGDPITDLLYSMNWLPIGFIQAYFITGDDYFKKLWKDVA
                     RFMMSAQIHSDNKAINGAWARAFDVELNEIYGMPKDRGWGPCVIESGWTVAEIVSGLI
                     MGMLEDKISQYYKQLA"
     gene            695..1876
                     /locus_tag="PK733_10905"
     CDS             695..1876
                     /locus_tag="PK733_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ11086.1"
                     /translation="MADISKEETTKVPYAPLGIIAMDGSKELCGMVDYYLTQWRGEYV
                     DSNENLNAYPGYRRNSFIVSASCHRFSSGEGKAVINDTVRGHDLYIISDIGNYNCTYK
                     MFGMDCPMSPDDHYQDIKRIISAIGGKARRITVIMPMLYEGRQHRRISRESLDCAIAL
                     QELERLGVDNILTFDAHDPRVQNAIPLIGFDNIHPTYQIIKALLKNEKDLVIDKSKMM
                     IISPDEGGMSRSLYYSSMLGLDLGLFYKRRDYSRVINGRNPIVAHEFLGDSVEGKDIL
                     IVDDLLSTGESVLDIATELKRRKANRIYVAVTFALFTDGLEKFQECYEQGIITKLFAT
                     NLTYRKEELKSAPWYVDVDMSKFIAYLIDTLNHDQSISSLLNPSEKINKLLDNYRANK
                     G"
     gene            complement(2086..3783)
                     /locus_tag="PK733_10910"
     CDS             complement(2086..3783)
                     /locus_tag="PK733_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ11087.1"
                     /translation="MSENSKFKIKVLSSLTKVFADEELRAFDWNTGSMLLNEVYSFQV
                     AYCWSEFMIKQVQVDVISELSPWITVRKVGLVPSEMPCYADHDDNVLRTTPGLYPDPL
                     MPIDADGITFLPEQWRSLWITVNPDGQVNPGSYPIAIVFKTSAGEELGKAEFTLEIIN
                     AALPKQSLINTQWFHTDCLATWYKVDIFSEEHWNIIKQYIQTAVKHGINMILTPLFTP
                     PLDTAVGGERPTVQLVDVEKLSGKCSDKLSDACSVTYKFGFKRLKRWVDMCLDNGVEY
                     FEFSHLFSQWGAKHAPKIIATENGGLTHLFGWDTDASGEEYKNFLDQFLPALIIFIKE
                     NGLERRSYFHISDEPSSAHYESYKNASDIIKKHVEGFPVIDALSDYKFYETGLVEKPI
                     PATNHIEPFLENNVPSLWTYYCCGQYKEVANRFFYMPSARNRILGLQLYKFNIYGFLQ
                     WGYNFWYSQYSRYPIDPFKVTDAGYAFPSGDAFMVYPGENGPISSLKFEVFYEALQDL
                     RALQLLEELIGHNEVIKLLEEGLDEPITFSRYPTDTEWLLLKREQINRKIANTMDIG"
     gene            complement(4021..4209)
                     /locus_tag="PK733_10915"
     CDS             complement(4021..4209)
                     /locus_tag="PK733_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ11088.1"
                     /translation="MADKKLVCKDCSEEFLFTEGEQAFYKERGFENEPQRCPACRKAR
                     KQNRNSNNNRSRSFGSRW"
     gene            complement(4512..5900)
                     /locus_tag="PK733_10920"
     CDS             complement(4512..5900)
                     /locus_tag="PK733_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ11089.1"
                     /translation="MLKDMTKGSETKHILVFAIPMFIGNIFQQLYNMVDAVVVGRYVG
                     KNALAAVGTSFPAMFLLIALVMGLAMGASVIISQLFGAGDLKKVKRAVSTTFIFLIIC
                     AIVLTFVGLFVSRDLLKLLKTPEEIIDDAAIYLNIIFSGLIFMFIYNTISAILRGLGD
                     SKTPLYFLIIASILNIVLDILFVTSLNMGVAGVAWATVISQAVSSVLCIIYVYAKVDL
                     LKIHPRDMVFDKELFVKSVKLGIPASIQQTVVSLGMMAIQGLVNSFGSVTMAAYTAAS
                     RIDSIAMMPIMNLGMATSTFTAQNIGAGEMERVRRGYKRSLAMVSVCCIVTSIVILIF
                     GPNLISIFIDAKEVDVITQGNDYITVVSFFYILMGLMFVTNGVLRGSGDMIVSMASTL
                     TSLGIRIIAAYILSSILMPGYKGIWWSIPIGWALGTTIAFIRYRSGKWVEKAVAGKPA
                     DSEQLKVAEDLS"
     gene            6280..7554
                     /locus_tag="PK733_10925"
     CDS             6280..7554
                     /locus_tag="PK733_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ11090.1"
                     /translation="MTQQNELSNLALYGGPKTRTKPFYGRGLIGPEEKAAVDELFDNS
                     ISSGEAFGYNGVEEEMYCKEFAEYMGGGYADAVNSGTTAVYVALRALDLEPFTEVIVS
                     SITDPGGMMPIALMNCIPVVADSAPGKYNTSAEEIEKLISPLTSAILVAHIGGEPCDI
                     DAIVALGKKHNIPVVEDCSQSHHAKLNGKLLGTFGDVAAFSTMYGKHHCAGGQGGMVF
                     TKNEELYWKIRRASDRGKPFGLPAGSTNSVASLNMNLNDLAAVIGRQQLKKLPGIVER
                     RRNIVAKLAEGFKNLKTLKIPEQLPGAEASYWWWRLEVNTDMITCDKETYCNALIAEG
                     ISLNPSYRSAMPHNMDWFKNRSVFGSSGYPWAAPEYKGDRNRQFPCPNAIDATGRQFN
                     LTVFESWGDEEVEDILKAFNKVEKAFLKYRNL"
     gene            8307..8855
                     /locus_tag="PK733_10930"
     CDS             8307..8855
                     /locus_tag="PK733_10930"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOJ11091.1"
                     /translation="MISKAIGEDSDKDAEEARNIEIKYEARDKTVFEIYNNWIIKCMI
                     YSVTAIDKNGKEPWNMPVLLNKPLLKFSMAGLLVAYIRGRNIYLIKDKVIKWQTVVGG
                     DIIYADINKDGFVSVVHKTEGYKALLDGITQLPLIKQFNKLGGFVFGALEGLLTVYII
                     FAVIMLFNSSPSFITVYKTIEQ"
     gene            8944..9942
                     /locus_tag="PK733_10935"
     CDS             8944..9942
                     /locus_tag="PK733_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ11092.1"
                     /translation="MKKLLGIDIGGTKCAIILGNYKDEAIEQTTSQYVTILDRITFPT
                     ETEEGLNYTLDRIFENIDIILNKNNCKAKNLSGIGISCGGPLDSKRGIILSPPNLVGW
                     DEVHIVETIEIKYGVKTKLQNDANACALAEWKFGAGRGYRNIVFLTFGTGMGAGLILD
                     GKLYSGTNDMAGEVGHIRLDSNGPVGYGKSGSFEGFCSGGGIAQLAKIKALEKFQLGE
                     KVSFCNSMDELNKLNAKIVAEAAKNGDELAKEIYAISGYYLGKGLSILIDILNPEIII
                     IGSIFSRSRDLLWPHAEKIIEKECLSYTKKVCKVVTSGLGEKIGDYAALAAAVSDL"
     gene            10131..10724
                     /locus_tag="PK733_10940"
     CDS             10131..10724
                     /locus_tag="PK733_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ11093.1"
                     /translation="MVCREDIISAFSTIVQCYEKGGKAMVCGNGGSAADSEHIVGELM
                     KGFILKREIPFKDKERIKAAFPENAEYLAKNLQGALPAISLVSQTAISSAFINDVAAD
                     MVYAQQVYGYAREGDVLISISTSGNASNVANAVKIAKAFGVKTIGITGKSGGMLRNLC
                     GVTIRVPAEETFRIQEYHLPVYHAVCAAVEEEFFGGK"
     gene            10736..11896
                     /locus_tag="PK733_10945"
     CDS             10736..11896
                     /locus_tag="PK733_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ11094.1"
                     /translation="MKKNATNSTDMKFGNRQLILNIIRKMPVSRAELARLTGLTRAAI
                     TLIVDELIHENILEEIGVAETSIGRKPILLDLNIKSHFAIGLYISREDCCISISNIKA
                     EPVLKMDVLLESYADAEECIAKIISQIDTLIADSKLTYERFIGIGVVTPGPVDIFTGT
                     ILNPPNFDNWHNVNIAQKLQEHFKLPVYLENYSASLALIEKNYGRGVEFENFMLMVVN
                     EGVGAGIIMQDKLYRGMGGFGHEVGHTTINFHGPKCSCGNRGCMEVYASIPSILKLIA
                     DSGTVLSSWQDVVDKALAGDEICKKAIEDEARYLAAGITNTINVLELEAVILTGDIIY
                     KPELLLNLINKNIENTAITRHLRKLPVMISSIACNIEVKAAVSVIIEKIFKK"
     gene            12147..12950
                     /locus_tag="PK733_10950"
     CDS             12147..12950
                     /locus_tag="PK733_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ11095.1"
                     /translation="MEILIEPLHKKNGLKAVVFDFDGTISTLRYGWEMIMEPLMLEMI
                     AGNTEIDDALKKKVREYIDQSTGIQTYYQMKWLAEAVKRYGRNPGASEDPWWYKAEYN
                     RRLMEPVNRRIESVKSGQKAASDFLIKGSKELLEALFNKGIEIYVASGTDDPDVKNEA
                     EILGLRRYFKEVSGAPLGRADCSKEAVLRKLVEDNRLHGTELAVIGDGKVEIALGNEV
                     GAVTLGIASNEADRCGINNSKRERLIRAGAHAIVGDFMQLGAILDWMDL"
     gene            12968..>13488
                     /locus_tag="PK733_10955"
     CDS             12968..>13488
                     /locus_tag="PK733_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11096.1"
                     /translation="MDKNIINPGQIDFSNIRTYPVKERVNLVTIDNMARPGIDEVPEW
                     FNRNFEELVDKIVQARRLRRPVIWSMGAHVIKNGMSRYLIELIKRGLITHVSGNGAGS
                     IHDFELAFNGGTSEDVPAAIEDGSFGMWEETGRWMNEALQEGAGKGLGYGESLAIYID
                     KNHEKFPYKEDCVL"
BASE COUNT         4529 a         2158 c         2785 g         4016 t
ORIGIN      
        1 tcacttttag ctagaaatgg tgatccaatc accgacctgc tgtactccat gaattggctt
       61 cctataggat ttattcaagc ttactttata acgggggatg attattttaa aaaattatgg
      121 aaggatgttg caaggttcat gatgtcagct cagattcata gtgataataa agcaataaac
      181 ggtgcctggg cacgggcttt cgatgttgaa ctcaatgaaa tttacggtat gcccaaggac
      241 aggggatggg gaccttgcgt tattgaaagc ggatggacag ttgcagagat cgtgtcaggg
      301 cttataatgg gcatgctgga ggataaaatt tctcagtatt ataaacaatt agcatagtct
      361 accggtgctt ccacctatta tagctatatc agcatgcatt tccttcaaca ttattattta
      421 cctccatata cttttttaac taaatttgca cgctataaaa aaggtcatgc agaattatgt
      481 agaaatattg tgatttttgt ataattaaag tataatataa attgtatgaa aattaattgt
      541 taaatggcaa gatattaatg aatttaatta tatcatacta acagattaaa ttatacaata
      601 actagattga agcaggagaa ctgtcctcaa gcttctacta catagaggca ggagaactgt
      661 ccctatgctt tcgataaatg gaggacacat aaaaatggca gatatatcta aagaagaaac
      721 aactaaagtg ccttatgccc ccctgggcat tattgcaatg gatggaagca aggaactctg
      781 cggaatggtt gattattacc ttacccagtg gcgcggcgaa tatgttgata gtaatgaaaa
      841 tcttaatgca tatccaggat acagaagaaa ttcttttata gtctcggcta gttgccaccg
      901 gtttagttca ggagagggaa aggcggttat aaatgataca gtaagaggtc atgatctcta
      961 cattatttct gatataggaa attacaattg tacctataaa atgtttggta tggattgtcc
     1021 tatgagtccc gatgatcact atcaggatat aaaacgcatt atttcagcta ttggcgggaa
     1081 ggcaaggcgt attaccgtta ttatgcccat gctctatgaa ggcagacagc acagaagaat
     1141 atcacgtgaa tcccttgatt gtgcaattgc gttgcaagag ttggagaggt tgggggtaga
     1201 caatattctt acttttgatg cccatgatcc aagagttcag aatgccatac ctttaattgg
     1261 ttttgacaat atacatccaa cataccagat tattaaagct ttactaaaaa atgagaagga
     1321 tcttgtaata gataagtcaa agatgatgat tataagtcct gatgaaggtg gaatgagcag
     1381 aagcctttat tattcctcaa tgctgggctt agatctggga ttgttttata agcgcaggga
     1441 ttatagcaga gtcattaatg gcagaaaccc tattgtagca catgaatttt tgggagattc
     1501 ggtggagggt aaagatatat taattgttga tgacttgctt tcaacaggtg agtcggtatt
     1561 ggatattgct acagaattaa aacgccgcaa agcaaataga atatatgttg cagtaacttt
     1621 tgctttattt accgatgggc ttgaaaagtt tcaggaatgt tatgagcagg gcattataac
     1681 caagcttttc gccactaatc taacatatag gaaggaagag cttaaaagcg caccatggta
     1741 tgttgatgta gatatgtcaa agtttattgc atatttaatt gatacattga accatgatca
     1801 atctataagc tccctcctta acccttcaga aaaaatcaat aaactgctgg ataattacag
     1861 agcaaataag ggatagaagg gttaaaggag taaaggagta aaggagtaaa ggatcagcga
     1921 aaagattgtg gtaataggtc aggattaaaa accaggacaa aagccagggg cagaagtcgt
     1981 cagaacaatg gctatgagca atattacagt acaaaacgct ggcacaaaga gaatcagtaa
     2041 tattacgggg tgcacccaat attactgatt ccctttgcaa ttcatttagc ctatatccat
     2101 tgtatttgca atctttctat taatctgttc ccttttcagc aagagccatt cagtatctgt
     2161 gggataccgg gaaaatgtta ttggttcgtc caatccttct tcaagaagtt tgataacctc
     2221 gttgtgacct attaattctt ctaataattg taaagctcgt aaatcctgaa gagcctcata
     2281 aaacacctca aactttaacg aagatattgg tccattctcc ccgggataaa ccataaaagc
     2341 atctcctgaa ggaaaagcat aacctgcatc agtgactttg aaaggatcaa taggataacg
     2401 tgaatattgt gaataccaga agttatatcc ccattgcaaa aagccgtaga tgttgaactt
     2461 atacaactgc aatcctaata ttctgtttct tgcagaaggc atataaaaga agcggtttgc
     2521 aacttcctta tattgcccgc agcaataata agtccatagg ctgggaacgt tgttttcaag
     2581 gaaaggttct atgtggtttg tagctggtat gggcttttca accaggcctg tctcgtaaaa
     2641 cttataatct gataaagcat caataacagg aaatccttca acatgtttct ttataatatc
     2701 gcttgcattt ttataagact cataatgagc tgaagaaggt tcgtctgata tatgaaaata
     2761 gcttcttctc tcaagaccat tttccttgat gaatattatt aaggcaggga gaaactgatc
     2821 caaaaaattc ttatattcct ctccagatgc atcagtatcc cagccgaata ggtgtgttag
     2881 ccctccgttt tcagttgcta taatttttgg agcatgtttg gctccccatt ggctaaatag
     2941 gtgagaaaat tcaaaatact ctactccatt atcaagacac atgtcaaccc agcgtttgag
     3001 ccttttaaag ccaaatttat atgtaacact gcatgcatca ctaagtttat cactgcattt
     3061 cccacttaat ttttcaacat ccaccagctg aacagtaggt ctttctcctc caacagctgt
     3121 atccagaggt ggtgtaaaca aaggagtcag gatcatgtta ataccatgtt ttactgcagt
     3181 ctgaatatat tgctttatta tattccagtg ttcttcacta aaaatgtcaa ccttatacca
     3241 tgtggcaaga cagtcggtat gaaaccattg ggtattaatc aatgattgtt ttggtaatgc
     3301 cgcattaatt atttccagag taaattcagc ttttccaagt tcttctcctg cagaagtttt
     3361 aaagactatt gcaatagggt aactacccgg attaacctgc ccatcagggt ttactgtaat
     3421 ccataaagaa cgccattgtt ccggtaaaaa agtaatgcca tcagcatcta ttggcataag
     3481 cgggtcagga taaagcccgg gagtagtccg cagaacgtta tcatcgtggt ctgcataaca
     3541 tggcatttct gaaggaacga gtccaacttt tctgacagta atccatggcg agagttcaga
     3601 aattacatca acctgaacct gcttaatcat aaattcgctc cagcagtaag cgacctgaaa
     3661 ggaatatact tcgtttagaa gcatggaccc tgtattccaa tcaaaagccc ttagttcctc
     3721 atcggcaaaa acctttgtta gagaacttag aactttgatt ttaaatttgc tattttctga
     3781 caatctaatc acctctccat tctgttgtaa tatatgggaa attatatata gaattatatc
     3841 tagtatatct actatatcta ggattatata gggattatat acaattatat ttgaaaagac
     3901 aatgggaaaa taaaaacaac aaaggagagc ttttgctctc ctttatatgt cggtattaaa
     3961 ttatttgctc aaacattttg ctcaaacaat tataattcta ttttgaaact agaattcaat
     4021 ttaccatctg cttccgaagc ttctgcttct attgttgtta ctatttctat tttgctttct
     4081 tgcttttcta caagcagggc atctctgagg ctcattttca aaacctcttt ctttgtaaaa
     4141 agcttgttct ccttctgtga aaagaaattc ttcgctgcag tctttgcaaa ccaatttttt
     4201 atcagccatg tctacatacc tccttaattt acttggatct tacctattct tgaattttct
     4261 actccaaata aattaaagag agttatattc aaaaaagtat caatccataa tacttacata
     4321 gaatagcatg ataattatat catgcggttt taaaatattc aataggttta aagatatttc
     4381 caaaaatatt tccagtaaaa aggacctatt tccagtaagt ttatagtata acacgagggg
     4441 caagttggga gcaagtcaag aactgtcctc ccttgctact ccttgttaca cttatcagtt
     4501 tctctgctta cttaagagag gtcttcagct actttaagct gttcactgtc tgcaggcttt
     4561 cctgcaactg ccttctcaac ccattttccg ctcctgtaac ggataaaagc tattgtagtt
     4621 cctaaggccc agccgatagg tattgaccac catattccct tgtatcccgg catcaatatg
     4681 gatgacagaa tataagcggc tattatcctt atcccaaggc tggtaagtgt acttgccatt
     4741 gatacaatca tatcaccgct gccacggagc actccattgg ttacaaacat tagccccatt
     4801 aatatataaa agaaggatac aactgtaata taatcatttc cttgagtaat tacatccact
     4861 tccttagcat caataaatat ggatataaga tttggtccaa atataaggat aactattgaa
     4921 gttactatac agcaaacgct taccatggca agggaacgtt tatatccacg tcttactctt
     4981 tccatttccc cggctccaat attttgtgct gtgaaagttg atgtggccat gccaaggttc
     5041 ataattggca tcattgctat agagtcaatt ctagatgcag cagtatatgc agccatagtt
     5101 acacttccaa aagaatttac cagtccctgg atggccatca tgcccaatga aactactgtt
     5161 tgctgaatgg aggcaggtat gccaagctta accgatttaa caaacagttc tttatcaaag
     5221 accatatctc tgggatgaat ttttaagaga tcaacttttg catatacata gattatacac
     5281 agtacagagg atacagcctg agatatgaca gttgcccagg caacacctgc aacacccata
     5341 ttaagtgatg taacaaacaa aatatcaagt acaatattta atattgatgc aataataaga
     5401 aagtataatg gcgtcttaga gtccccaaga cctcttaata ttgctgatat tgtattatag
     5461 ataaacataa atattaatcc gctgaagatt atattcaaat atatggctgc atcatcaatt
     5521 atctcctccg gtgttttaag aagctttaaa aggtctctgg agacgaacaa tccaacaaag
     5581 gtaagcacta tggcacatat tataaggaat atgaaggttg tggatactgc acgtttaacc
     5641 ttttttaaat cacctgcacc aaaaagttgt gaaataataa cagaagctcc catagcaagc
     5701 cccattacaa gagcaattag aagaaacatg gcaggaaagc tggtaccgac cgctgccaga
     5761 gcatttttgc caacatacct tcctacaaca acagcatcta ccatattata aagctgttgg
     5821 aatatattac ctataaacat tggtatggcg aataccagta tatgctttgt ttctgaacct
     5881 tttgtcatgt cttttagcat ttcttaatgt cactcctcaa aaaatagttc cgtatgcaat
     5941 acacgaatat ataatttcat aatatatagt tttatagttt cataatatat agttccataa
     6001 tatatagttc tataatatga cacagtgaat gctcatgcat atttatttgc tgcaatttta
     6061 cactgtgacg tgtggaatat aatgaagtat attagagtat attgtatcag ttatttgccc
     6121 aacaaggcaa ttgttttttt tatttttgct gaaaaggttt attttagagt aaaatatgcg
     6181 aagctaataa tgaaatgaag atgtattatt gaaatagatg tattggtaaa ttaaatgtca
     6241 aattcatatt ggttatattg attaatagga ggtagtaata tgacacagca gaatgagctt
     6301 tcaaatctgg ctttatatgg aggaccgaaa acaagaacaa agccttttta tggcagaggt
     6361 ttaattggac ctgaagaaaa agctgcagta gatgagcttt ttgataattc aatatcaagt
     6421 ggtgaagcat ttggctataa tggtgttgaa gaagaaatgt attgcaagga atttgccgaa
     6481 tatatgggcg gtggatatgc agatgctgtt aattcaggaa caactgcagt ttatgtggct
     6541 ttaagagcat tggatcttga gcctttcaca gaagtaattg taagttctat taccgatccg
     6601 ggcggaatga tgcccatagc tcttatgaat tgcattcctg tagttgctga ttcagctccg
     6661 ggaaaataca acacaagtgc agaggaaatt gagaagctta tctctccatt aacaagtgcc
     6721 attcttgtag cgcatatcgg tggtgaacct tgtgatattg atgctatagt agcattaggt
     6781 aaaaaacata atatccctgt agtagaagat tgttcacagt ctcatcacgc aaagctaaat
     6841 ggtaaactat tgggtacatt cggtgatgtt gcagcttttt caactatgta tggaaagcac
     6901 cattgtgcag gaggacaagg tggaatggtt ttcacgaaaa atgaagagct ttactggaaa
     6961 attagaagag catcagaccg tggaaaacca tttggacttc cggcaggttc tacaaactca
     7021 gtagcttcat taaatatgaa tcttaatgat ctggcagcag ttataggaag acagcagctt
     7081 aagaaacttc ctggaatagt tgaacgtcgc cgtaatattg tggcaaaact tgcagaaggt
     7141 tttaaaaatt tgaaaactct taagattcct gagcagcttc ctggtgcaga agcttcatat
     7201 tggtggtgga gattggaagt taatacagat atgataacat gtgataagga aacatactgt
     7261 aatgcgttaa tagcagaagg tatttcactc aatccatcat acagaagtgc tatgccccac
     7321 aatatggact ggttcaagaa ccgttctgta tttggatcta gcggatatcc atgggcagct
     7381 cctgaatata agggtgatag aaaccgtcaa ttcccatgcc caaatgcaat agatgctaca
     7441 gggcggcagt ttaatctcac agtttttgaa agctggggag atgaagaggt tgaagatata
     7501 ttgaaggcat ttaacaaagt tgaaaaggca ttcttgaagt ataggaattt ataaaaaatt
     7561 cctatactga ggtttggaaa ggggattccc cttcccggtt taaggagtag atgggcgttg
     7621 tagagcgagc aggcgatttt gcttcgcaaa acgctttggt gctcaggatt ctgcaataaa
     7681 cattaatgag gcagaatccg tcgaagggaa cctagggttc cctagcgaac aagaaaattc
     7741 gcatcattgt tttaggtcat gagtgaagcg aattttcttg aagaaatagg gtacaaatta
     7801 aactttaaag aaaaataatt aatccgggca ggtttttcat aaaggatttc ctgcccggat
     7861 atgtgtctgc caggaaagta caaatctgct ttaaaccgta tggaactggt gaaaattact
     7921 gatgtaagga aaagaacata ctattatact aatttcccat atactgtcag aaattagcgc
     7981 agtttgtgaa agagttgtta taatccacaa tggggaaact gagaaaagta aagattaaaa
     8041 ttaatgtaaa tcagattcaa gctataacta tctctcttaa caggctctgg catattggta
     8101 attctaatga ttgctaatat tccggagttt tttatatgca taagaaagta taattctacc
     8161 ttaaatcgga gaagccgact tttttatggt gggtatattt taatattaca taatctatgg
     8221 taaaatgttt cttataatat atacaatcag gaggagcatt ggtactttgc agaatgaaaa
     8281 aaagaggcga agaacagtaa aaaatattga tatctaaagc aataggcgaa gattctgata
     8341 aagacgctga ggaagccagg aacattgaaa ttaagtatga agccagggat aaaaccgttt
     8401 ttgaaatata caataattgg attattaagt gtatgatata tagtgtaaca gctatagaca
     8461 agaatgggaa ggaaccgtgg aatatgcctg tattactgaa caagcctttg ctaaaattca
     8521 gcatggcagg gttgcttgta gcatatataa ggggaagaaa catttacctt atcaaagata
     8581 aagtcataaa gtggcagact gttgttggtg gtgatatcat atatgctgat attaataaag
     8641 atggatttgt ttctgtggta cataaaacag aaggttacaa agctttattg gatggaataa
     8701 cacaactgcc cctaatcaag cagtttaata aattgggtgg ttttgttttt ggtgctctgg
     8761 aaggactgct aacagtatat ataatttttg cagtaataat gttatttaat tcatcaccgt
     8821 cttttatcac ggtatataaa actatagagc agtaaatgat tgcaaaatat ttcttttgta
     8881 gtttaggagg ctgctcaaga aataatataa ttaattcaaa atacaagttt gtaggtgata
     8941 agaatgaaaa aactcctggg aattgatatt ggtggaacaa aatgcgccat tattctaggg
     9001 aactataagg atgaagctat agaacagaca acttcacaat atgtaacgat acttgataga
     9061 atcacctttc caacagagac agaggaaggt cttaattata cacttgatag aatatttgaa
     9121 aatatcgata ttatattaaa taagaataac tgtaaagcta agaatttatc aggtataggt
     9181 ataagctgcg gagggccgct tgacagcaaa aggggaataa ttttatcgcc tcccaactta
     9241 gtaggatggg acgaagtaca tattgtagaa acgattgaaa taaagtatgg agtgaaaact
     9301 aaacttcaga atgacgctaa tgcatgcgct ctggcagaat ggaaatttgg tgctggaaga
     9361 ggatatagaa atattgtgtt tcttactttc ggcacaggca tgggtgcagg ccttatactt
     9421 gatggaaagc tctacagcgg tacaaatgac atggctgggg aagtgggaca tataagactt
     9481 gatagtaacg gacctgtagg atatggcaaa tcaggctctt ttgaaggttt ttgcagcgga
     9541 ggaggtattg ctcagttagc aaaaataaag gctctggaaa aattccagtt aggagaaaag
     9601 gtatctttct gcaacagcat ggatgaattg aataaactaa atgcaaagat tgtagcagag
     9661 gctgccaaaa atggagatga gctggcgaaa gaaatatatg caatatcagg atattatctg
     9721 ggaaaagggc tttcaatatt aatagatatc ttaaatcctg aaataatcat tataggtagc
     9781 attttttcca gaagcagaga cttattgtgg ccacatgctg agaaaatcat agaaaaagaa
     9841 tgcctcagct atactaaaaa agtatgtaaa gtagtaactt caggacttgg tgaaaaaatt
     9901 ggcgactatg ctgctctggc agcagcagta agtgacttgt aaagtcagtt ttcgcatata
     9961 aacaggaggc attgtgatac tttaatggaa aagatttgta taaaaaataa gataatatta
    10021 ctataatgat tagtatagta atatgaatat attaataagg tggtgttata catatgaaaa
    10081 aggctgtaga tgagattttt aacaggttat ttttaaattt tcctgagctg atggtatgca
    10141 gagaagatat aataagcgct ttcagtacta tagtccaatg ttatgaaaag ggcggaaagg
    10201 caatggtttg tggaaatgga ggaagtgcag ctgactctga acacattgtg ggcgaattga
    10261 tgaaagggtt tattctaaaa cgtgaaattc catttaagga taaagagaga ataaaagctg
    10321 cttttccgga aaacgcagaa tatctggcta aaaaccttca aggggcttta cctgcaattt
    10381 cattggtaag tcagactgcc atttcatcag cctttatcaa tgatgtagca gctgatatgg
    10441 tatatgcaca gcaggtatac gggtatgccc gcgaaggtga tgtattgatc agcataagta
    10501 catccggtaa tgcttctaat gttgcaaatg cagtaaaaat tgcaaaggct tttggtgtta
    10561 aaacaatcgg aattacaggt aaaagtggag gtatgctgag aaatttatgt ggcgttacaa
    10621 ttagagtgcc ggcagaagaa actttcagaa ttcaggagta tcatcttcct gtataccacg
    10681 ctgtatgtgc tgcagtagaa gaagaattct ttggtgggaa gtgagacgga tttcaatgaa
    10741 aaagaatgca acaaattcaa cagatatgaa atttggtaat aggcagctta ttttgaatat
    10801 tatcagaaaa atgccggtat caagggctga attagctaga ttaactggcc ttacgcgtgc
    10861 tgcaattact ctgattgtcg atgaattgat acatgaaaac atattggaag aaatcggagt
    10921 agcagaaaca agtataggaa gaaaaccaat actgctggat ttaaatatta agagtcattt
    10981 tgccattggc ttatacattt cacgtgaaga ctgctgtatc agtatttcaa atattaaggc
    11041 ggagcctgta ttgaaaatgg atgttttgct ggaatcatat gctgatgcag aagagtgcat
    11101 agccaaaatt attagtcaaa ttgatacgct tatagctgat tctaagctca catatgagag
    11161 attcatagga ataggtgttg ttacaccggg gccggttgat atttttaccg gtactatcct
    11221 caatcccccg aactttgata attggcataa tgttaacatt gctcaaaagt tacaagaaca
    11281 cttcaagctt cctgtatacc ttgagaatta ttcagcttct cttgccctca tagaaaaaaa
    11341 ttacggaaga ggagtcgaat ttgaaaactt tatgcttatg gtagtaaatg aaggagttgg
    11401 tgcaggaatt attatgcaag ataaactata tagaggtatg ggaggattcg gacatgaggt
    11461 aggtcataca actataaact ttcatggacc aaaatgtagc tgcggcaata gaggctgcat
    11521 ggaagtttat gcatcaattc cttcaattct taagttaatt gccgactcag gtactgttct
    11581 atcttcatgg caggatgttg tcgacaaagc cctggctgga gatgaaattt gtaaaaaagc
    11641 cattgaagat gaagcacgtt atcttgccgc aggtattaca aatactataa atgttctgga
    11701 attagaagca gtgattttaa caggagatat tatctataag ccggagcttt tgctaaacct
    11761 tataaataaa aacatagaaa atacagcaat aaccaggcat cttcgcaaat tgcctgttat
    11821 gatctcatca atagcttgca atatagaagt aaaagcagca gtgtctgtga taattgaaaa
    11881 gatttttaag aaatagaaat aagagttaat agattagact gttttccaaa tgattttttt
    11941 aaaaaaagta ttgctaaata acgtaatgac gaatataatg tttacatagt taaatcattt
    12001 aacaaatgta ttgctattta ttatatgtac gtaaaccaat tacctttata tatattgact
    12061 aatatgcaaa tttacctaat gggtaaattt atctgatttt gaatggtatc taattcatat
    12121 tgagaaggag aaaaagggga aatagaatgg aaattttaat tgaaccactt cataaaaaaa
    12181 atggtctgaa agcagttgta tttgattttg acggtactat atcaacctta cgctatggtt
    12241 gggagatgat tatggagcct cttatgcttg aaatgattgc aggaaatacg gaaattgatg
    12301 atgctttaaa aaagaaagta agagagtaca tagatcaatc aacaggtatt caaacatact
    12361 atcagatgaa atggctggca gaggcagtga aaagatatgg cagaaatccc ggtgcaagtg
    12421 aggatccctg gtggtataag gcagaatata atcggaggct tatggaacca gtgaacagaa
    12481 gaattgaaag cgtaaaaagt ggtcaaaaag cagcttctga ttttttaata aaaggaagta
    12541 aagaacttct tgaagcttta ttcaacaagg gaatagaaat atatgttgca agtggaactg
    12601 atgatccgga tgttaagaat gaagctgaga ttctcggctt aagaaggtac ttcaaagagg
    12661 tttcaggtgc accattaggt agagctgatt gttcaaagga agctgttctg cgaaaacttg
    12721 tggaagataa tagattgcat ggtacagaac ttgctgttat tggtgacgga aaggtagaga
    12781 ttgctcttgg aaatgaagtc ggagctgtaa ctcttggtat agcaagcaat gaggcagata
    12841 gatgcggaat aaacaattca aaaagggaaa ggctcataag ggcgggagct catgctattg
    12901 ttggtgattt catgcaactt ggtgcaatac tggattggat ggatttgtag aagggggaat
    12961 gtgtacgatg gacaaaaata taataaatcc cgggcagata gatttctcga atataaggac
    13021 atacccggtt aaagaaaggg ttaatcttgt tactattgat aatatggctc gacccggaat
    13081 tgatgaagtg ccggaatggt ttaatcgcaa tttcgaagaa ttagttgata aaattgttca
    13141 ggccagaagg ttgcgaagac ctgtaatatg gtccatgggg gcccatgtaa taaaaaatgg
    13201 aatgtccaga tatttgatag agctaataaa aagagggtta attacccatg tatcaggaaa
    13261 cggagccgga agtattcatg actttgaact tgcttttaac ggaggtactt cggaagatgt
    13321 acctgcagca attgaggatg ggtcttttgg catgtgggag gaaaccggaa gatggatgaa
    13381 tgaagccctt caggagggcg ccggaaaagg actcggatat ggagaaagcc tggcaattta
    13441 catcgacaaa aatcacgaga aatttccata taaagaagac tgtgtact
//
LOCUS       DAOSDD010000113        13363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1803_length_13363_cov_5.028329, whole genome shotgun sequence.
ACCESSION   DAOSDD010000113 DAOSDD010000000
VERSION     DAOSDD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_13363_cov_5.028329"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..633)
                     /locus_tag="PK733_10960"
     CDS             complement(10..633)
                     /locus_tag="PK733_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11097.1"
                     /translation="MNAVQLVLSVLIHPIDTFRIIKSRKLKINYLQLSSLLLFMVVVV
                     RIIYIYMLHYPLSNLTIRDTNVLLEIIRMTVPIITWVVACYALTSIMDGETTFLETLI
                     SATLSMMPYIFFIIPFALLSKIMSGTDIGTFNLILKFIWIWVAMLIIIGVKELNDYSI
                     IKTLNICVLNLITMILICAIIVLFLALASQLFTFINELFLEIRLIFN"
     gene            complement(650..2083)
                     /locus_tag="PK733_10965"
     CDS             complement(650..2083)
                     /locus_tag="PK733_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOJ11098.1"
                     /translation="MLRQMLRRICIGVALISLFFNTVYVSYVSAADINYVLDGKQRVP
                     IPLTYKVKNVIDKLDSNTNTLKQPEDIFIDRYDNIYIADTGNNRIVKYNSDGKLIYVY
                     GDEDEKDKQFNNPMGLFVDDDEDIYVADTANNCIAHLSPYGEFVEQFVKPESLLLNDS
                     FTFEPTKIYINSTGFIYILKSHSFVTIDADNNFRGYVGANQVEFDFTKVLIRLFASKE
                     QKERIAKNLPDSYTNFVIDKNGVIYATCYTEKQNQIVKLNSVGKNIFRKGSFGETIDD
                     NNRPIKPRFIDIAVDANGIINALDQISGKVYQYDQEGNLLTVFGGIGTWKGTFQMPSS
                     ISVDSHGNIYVLDRILNNVQVFEPTSFIKLIHEAIGYYDEGEYVKAKDIWNEVIKFDK
                     NYKLAHTGIAKVLMKERKWKEAMNEYEMADDKAGYTKAFNEYRKSAIQEYFGLIILIV
                     IVIITILIIITGKVKNAIGNLIENFKM"
     gene            complement(2097..2912)
                     /locus_tag="PK733_10970"
     CDS             complement(2097..2912)
                     /locus_tag="PK733_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11099.1"
                     /translation="MYLFMLLLVAFTALPLVYMVSTAFKPIDELFIYPPQFLVRRPTL
                     ESFTDLFTALDSSVVPFTRYVFNSLFTTIIIVSGTVIISSMGAFAMGKYNLPGTGILF
                     NIIILALMFSPYTTQIPSYMIVNKLGMINTYWALIIPKLAVAYNFFLMKQFSSQIPDA
                     LLEAARIEGATEWRIFWKIAMPILQPAWATLIVFSFVNNWNDYFTALIFTSSQVMKTL
                     PLALQTIAGGPGAIARSGASAAATFIVTLPTILIFTIMQAKVMKTMAYSGIKA"
     gene            complement(3010..3963)
                     /locus_tag="PK733_10975"
     CDS             complement(3010..3963)
                     /locus_tag="PK733_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ11100.1"
                     /translation="MSTVINNKVINIGKKPSFNNKKAISKWLNKYGIGYLFMLPFIIL
                     FFTFIIIPVFVSIGLSFTQYNMIQLPKWAGLVNYKTLLTKDDIFLLALKNTMFFALIT
                     GPIGYIMSFIAAWIIDQLKFRNAFSLAFYAPSITSGIALSIVWLYFFSGDRYGLINNI
                     LINFGFIKEPVLWNINPKTIMPVIIIISVWMSMGTGFLVFLAGLQNVPKELYEAGAID
                     GIKNKFQELYYITLQLMKPQMLFGAINSIVGSFGVFDIAVSVAGMPSPNYAGHTIVAH
                     LYDHAFIRFEMGYASSISVILFLITFILGRLSMKLFSTRDE"
     gene            complement(4004..6943)
                     /locus_tag="PK733_10980"
     CDS             complement(4004..6943)
                     /locus_tag="PK733_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11101.1"
                     /translation="MVEKRWFSGNNLYKRICIILVLSILTFSSYVSGEPGTKLKEKFT
                     AKTENIYKEIDEYLDSEPSYSLIQKLYEKKGYMPTQNIEIILDMDSIVASSTGDIPLT
                     ANIGGRQEPVLLWTDTYDWFEWSVDITHEGLYEILMEYYTIAGSCSDVYRSIKIDNDV
                     AFSEMYNIRFPLIWEYTGKPTVNNIGDEVLPRQEQVLEWRTTAVVDSEGKYAQPLIFY
                     FSEGKHTIRMSFINQSVVFGNMIIKSPERIKTYDEVKNSYEKNGYNYVDKTIKFQAEE
                     AVLGKSNQTIKMVTDGDPLTEPVYNDTRKLNAMGGWSWRKGNDTITWEFNIPEDGLYN
                     IGFRAAQWWSKGIPVYRQIAIDDCIPFEDMLLYKFDYSKNWKMEILHDKDDEPYLFYF
                     KKGEHKLTMTVKLGPIAEIADSLKVDLLMLSDILRQIIMLTGNNPDYNFEYELERNIP
                     HLTDNLEILMESMQSKIDILNSISTDKPTFINDLLLIKDQLRKMIADPDSITRKLNDL
                     SNTQTILGSWLLCFQDQPLIIDYVNIGKYDKKWDIVQSNILQKVRKTWQNFVRSFIKD
                     YDSVGGKNTSKERTTLDIWVSNGKEWAEIMKSLADEDFTSKTGISINLNVLPSSQVNA
                     GTVNALMLAVCSGEAPDIASGMAANSPVEFAIRDSVVDISQFSEFSAIVKDRFFEGVM
                     IPFKYRNGYYALPETMNFNVLFYRKDIINELGIDIPQTWKEVYENVLPILYRNGMKFY
                     CPQDFSTFLYQNNGKFYNETGTLSGLDTPEAYKAFKEWTELYTSYGVPVVSNFFSRMR
                     TGDTPMGIGGYTNYIQFMSAAPELIGKWGIALIPGHEDINGNIKRASGGIEGQALVIM
                     NQSKHKNEAWEFLKWWTSTDVQTRFGNELEALMGIEARWNTANKEAFKELPWKTEDTK
                     IIMNQISNSIAQPVVLGGYFTNRHIVNAWNSVVLGGENVRDSLEKAVIEINKELQSKQ
                     EEYGVVN"
     gene            complement(7263..8900)
                     /locus_tag="PK733_10985"
     CDS             complement(7263..8900)
                     /locus_tag="PK733_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11102.1"
                     /translation="MKKIISLLLAVAMIFTISACTVKKANDSDGRVDITTAGKDPANT
                     ASNTEKEQVYTGQLPLTKEKVKLVFAVPAPGSTTIPPASIELPVYKKYEEITNVEVEW
                     EVIMKDYDATIKSRIAAGTDIPDMFYVSLADAPQFVEDGVIVQLDDLVEKNAPYLMKY
                     VKENPSEIKLRTSDDGHFYFLAGYSIINANAVTPLLNVKWVERLNQEIPKTLDDFYNI
                     LKLFKTNDLNNNGQNDELPLCGWMASNSADGSLGNSFGLELSVGGFQLDKNGKVTCDF
                     VNPRYKEYMAYINKLYKEDLWHKKEALKTVETYEYAKSNIMGCTITYSTFAGIMSGLS
                     PETNDTTGNEPVFVPLDPLRGPYGDQYFIAGSSSISSGVAVSGKCKNKDIAVRYIDFL
                     SRNPEAIILQNYGVEGITYDKVNGKIVVRESPNGVPFNEFLQSVGGGQPSFPHTQDYE
                     AWKTKYCTWVMDINDAQQKYFNKPSLLRIRFTKEETDNLNLIWPDMNKYIQDMKVKFM
                     TGQEPLDKYDDYVAQVKGMGLDKVLSIYQSRQDRAAK"
     gene            complement(9211..10461)
                     /locus_tag="PK733_10990"
     CDS             complement(9211..10461)
                     /locus_tag="PK733_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11103.1"
                     /translation="MNQNKNIRVGVVGIKRGLEYIKDINKHSSIFNMELAAICDIEEK
                     LLNEISNQYKVKGYTDYAKFLEHDMDAVILCNYFHEHAPFAIKALQAEKHVMSEVGAC
                     KTLGEGVSLARAVEKSNKIYLFGENYPYTRYIQEMRKLYKEDAVGEIQFGEGEYIHPC
                     SADGFNQLAMGLDHWRNVKPATYYCTHSIGPLMYITDTRPTSVNALCIPFSEKDEQKL
                     HVRIHDIAATIMCRMNNDAVVVLNGVSLRGDGNWFRVHGTRGFMENMRTGNKQMVRIK
                     HNSMDLKPDEEVEKTYLPNFPDFNDIAPSSSHNGADFFVLYNFAEAIKTNKQPYHDVY
                     KGLDMTLVGIQAYKSALANGAPFEIPDFRDENVRKRYENDNWSPYPEDYAPGQPYSSI
                     KGKITPSQEAIENAKRIWIERPYH"
     gene            complement(11095..12090)
                     /locus_tag="PK733_10995"
     CDS             complement(11095..12090)
                     /locus_tag="PK733_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOJ11104.1"
                     /translation="MEEKIAIISKSQVEKYLSVSSVIDRVEQTYKWYAEDKIIMPSKI
                     TLDMASLNVPGWINSMPSYVQPCDIAGIKWVGGFINNHKIGLPFIKAKIMLTDPRTGL
                     MKALVDGDWISDMRTGAQTAVFAKYLAPKNTDIVAIIGAGLQGYTTLVCLCELFKFKE
                     VRICDIYSEACKNFIAKAEKTVPCEIKAYNSTEEAVRDADIIVTATTADAPLVMNDWV
                     KPGALVSTIGSYQELDEELVFKADKVIVDHIEQHLHRGEFVKLFEEGRMKENDFYGEF
                     PNIITGSLNGRESEKERIVISIVGMGCLDVSIAAMLYDIAVKDPENKLLIYNLAE"
     gene            complement(12194..>13363)
                     /locus_tag="PK733_11000"
     CDS             complement(12194..>13363)
                     /locus_tag="PK733_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11105.1"
                     /translation="NYLFEKGLDKILKKVGEELNVPTYTEYDKFLEHDMDAVVLANFF
                     NEHAPFALKALEAGKHVISETSCNATIADGVALCRAVEKSGLVYMLAENYPYMKFNQE
                     MRKLYRQGEIGEVTYADGEYNHPMDARSRLRISPGKNHWRNHIPPTYYNTHALAPLMY
                     ITDTMPKKVNGLSIACREIDKETMRVRDPGGVILCRMDNGAVFRLFGLSLPGHSTWYR
                     LHGSKGAMERIRSSAHYASDKIRIWHEEWDLEPGKVIEKEYIPEWPEHGELAQKAGHG
                     GGDFWTNFYFAEAIRTGQQPYLDVYRGVTMSTVGILAWKSALQDGVPFDVPDFTSECE
                     RKKYENDKWSPFPEDAGPGQPLPSILGKIEPSEEAKKLAKEVWSGIGYKDVDNLV"
BASE COUNT         4071 a         2682 c         1861 g         4749 t
ORIGIN      
        1 tatattagcc taattgaaaa tcaatcttat ctctaaaaac aattcgttta taaacgtaaa
       61 taactggctt gctaaagcta agaataatac tataatagca catattaaaa tcattgtaat
      121 aagattaagc acgcatatat tcaatgtttt tataatagaa taatcattta attctttaac
      181 gccaataata atcagcatag ctacccaaat ccatataaat ttcaaaataa gattgaatgt
      241 acctatatcg gtgccggaca ttatttttga cagtagtgca aatggtataa tgaaaaaaat
      301 atatggcatc atagataggg ttgctgaaat taaagtctcc aaaaaagtag tttctccatc
      361 cattatagaa gtaagcgcat aacatgcaac tacccatgta attattggta cagtcattct
      421 tattatttcg agtaaaacat tcgtatctct tatagtaaga ttgcttaaag gatagtgaag
      481 catatatatg tatattatac ggacaactac taccataaat aataataacg aagacaactg
      541 caaataattt attttcaact ttctggattt aataatcctg aatgtatcta taggatgtat
      601 aaggactgat agaaccagct ggactgcgtt cattatttac ctccccctgc tacattttaa
      661 aattctctat gagattacca atagcatttt ttactttgcc tgttataatt attaaaatag
      721 taattattac tattacaata aggattatca agccaaaata ctcttgaata gccgattttc
      781 tgtattcatt aaatgctttt gtataacctg ctttatcatc agccatttcg tattcattca
      841 ttgcttcttt ccattttctt tccttcataa ggacttttgc aatgcctgta tgggcaagtt
      901 tatagttctt atcgaattta ataacctcgt tccatatgtc ttttgctttc acatattcac
      961 cttcatcata ataccctatg gcttcatgaa ttaattttat aaagcttgta ggctcaaaaa
     1021 cctgtacatt gttaagtatt ctatctaaaa catatatatt tccatgtgaa tctactgaaa
     1081 tacttgatgg catctgaaaa gtgcccttcc aagtccctat acctccaaat accgtaagaa
     1141 ggtttccttc ctgatcgtat tggtacactt ttcctgatat ctgatcaagt gcattaatta
     1201 ttccatttgc atctacagct atatcaataa aacgcggttt gatgggtctg ttattatcat
     1261 caatagtctc tccaaacgac cccttcctaa atatattttt ccctaccgaa tttaacttta
     1321 caatttgatt ttgtttctct gtgtaacatg tagcatatat aaccccattt ttatctatta
     1381 caaaatttgt atatgaatcc ggaagatttt ttgcaattct ttctttttgt tcctttgaag
     1441 caaaaaggcg gattaagact ttggtgaaat caaactccac ctgatttgcg ccaacatagc
     1501 ctcgaaagtt gttatcagca tcaattgtga caaaagaatg gcttttaagt atataaataa
     1561 agccggtaga gtttatatat atctttgtcg gttcaaatgt aaaagaatca tttaaaagca
     1621 aagattccgg tttcacaaat tgctcaacaa attccccata aggtgaaaga tgcgctatac
     1681 agttattcgc cgtatccgct acatatatat cttcatcatc atcgacgaac aagcccattg
     1741 gattattaaa ctgcttatct ttttcgtctt cgtctccata tacatatatc aattttccat
     1801 cactattata tttgacaatt ctgttattac ctgtatctgc aatatatatg ttatcatacc
     1861 tatctataaa aatatcttcc ggttgcttta aagtgttcgt gttgctatct agtttatcta
     1921 ttacattttt aactttatat gttaaaggga tcggtacacg ttgttttcca tccaatacat
     1981 aatttatatc cgctgccgat acatatgata catatacagt attgaaaaaa agtgatatta
     2041 acgctacacc gatacatata cgtctcaaca tttgccgcaa cattttttcc tcctcattac
     2101 gcttttatgc ctgaatatgc cattgttttc attacttttg cctgcataat tgtaaatatc
     2161 agaatagttg gtaatgttac tataaatgtg gctgctgccg aagctccact ccttgcaata
     2221 gctcctggtc cgccagctat tgtttgaagt gcaagaggca acgttttcat aacctggctg
     2281 cttgtaaaaa tcaatgccgt aaaatagtca ttccagttgt taacaaagga aaatacaatc
     2341 aacgttgccc atgcgggctg caaaattggc atagctatct tccagaatat tctccattca
     2401 gtagctcctt caatacgtgc tgcttctaat aaagcatcag gaatttgaga agaaaactgc
     2461 ttcataagaa agaaattgta tgctactgcc agtttaggta ttattaatgc ccaatatgtg
     2521 ttaatcatac ctaatttatt cactatcata taagatggta tttgtgtagt atatggtgaa
     2581 aacatcaatg ccaagataat tatgttgaat aatatacctg taccaggcag attgtatttc
     2641 cccatggcaa aagctcccat gctagatatt attacagtgc ctgatactat tattattgta
     2701 gtaaacaagc tattgaatac atatctggta aatggcacaa ctgagctatc taatgctgtg
     2761 aaaaggtccg taaagctttc tagtgtcggt cttctaacaa gaaactgcgg aggataaata
     2821 aataattcat ctattggttt aaaagcagtt gataccatgt acactaaggg taaagccgta
     2881 aatgctacta atagcaacat aaataagtac actaaaaacc acccaaagct aaacttgaac
     2941 ttaaacctaa tatacaagca gttttttatt ttcttcaaag aatatttaat atgcatgtat
     3001 tatctcctct tattcatctc tggttgagaa taatttcata cttaatcgtc caagaataaa
     3061 agtaattaaa aacagaataa cagaaattga agaagcatag cccatttcaa atcgtataaa
     3121 agcatgatca tataaatgag caacaatagt atgtcctgca tagttagggc taggcatgcc
     3181 tgctacagaa actgctatat caaaaacacc aaaagagcca acaattgaat tgattgcacc
     3241 aaatagcatc tgtggtttca taagttgtaa ggtaatataa tatagctcct gaaatttatt
     3301 ttttatacca tctattgctc ctgcttcata taactcttta ggtacattct gtaatcctgc
     3361 aagaaatact agaaatcccg tacccatgct catccataca gatattatta taataaccgg
     3421 cataatagtt ttggggttaa tattccacaa tacaggttct ttaataaacc caaaattgat
     3481 taggatatta ttaatcaatc catatctgtc tcctgaaaag aaatagagcc atactataga
     3541 aagtgcaatt ccactggtaa ttgatggagc gtaaaaagcg agtgaaaatg catttcggaa
     3601 tttaagctgg tctattatcc atgccgcaat aaacgacatg atatacccta taggtccggt
     3661 tattaatgca aaaaacattg tattctttaa tgccaataaa aatatatcat cttttgttaa
     3721 taatgttttg taatttacta acccagccca tttaggaagt tgtatcatat tatattgggt
     3781 aaaacttaga ccgatagata caaataccgg gataataata aatgtaaaaa ataaaatgat
     3841 gaatggcaac ataaataaat aaccaatgcc atatttattc aaccatttac taattgcttt
     3901 tttattatta aatgatggtt ttttaccgat atttattacc ttattattaa ttactgtgga
     3961 catattactc tctccatttc attaaacgaa aattttaata ttttcaatta acaactccat
     4021 attcttcctg tttagattgc aattctttat ttatctcaat cactgctttt tcaagtgaat
     4081 cgcgaacatt ttctccacca agtactacgc tattccatgc atttactata tgccggtttg
     4141 taaaataacc tcctagcact accggttgag caatagaatt actaatttga ttcataataa
     4201 ttttcgtatc ctcagttttc catggtaatt ctttaaaggc ttccttattt gccgtattcc
     4261 atctggcttc tatacccatt agagcttcca attcatttcc aaagcgtgtc tgtacatcag
     4321 tactggtcca ccatttaaga aattcccatg cttcattctt gtgttttgat tgattcataa
     4381 taactaatgc ctgtccttct atgcctccgg atgccctttt gatatttcca tttatatcct
     4441 catgacccgg tataagtgca attccccatt tcccaattaa ctcgggtgct gcagacataa
     4501 attgtatata atttgtataa ccgccaatac ccatcggtgt atcacctgtt ctcatcctgc
     4561 taaaaaagtt tgagacaaca ggtactccat agcttgtata taactccgtc cattccttaa
     4621 acgctttata tgcttcaggc gtgtccagcc cggacaatgt tccggtctca ttataaaatt
     4681 taccattatt ttgataaaga aatgtagaga aatcctgcgg gcaataaaat ttcataccat
     4741 ttctgtaaag gatcggcagc acattttcat atacctcttt ccaagtttgc ggtatatcaa
     4801 tacctaattc gtttattata tctttcctat aaaataacac attaaaattc atcgtttccg
     4861 gaagtgcata atatccattt ctatatttga acggtatcat cacaccttca aagaacctat
     4921 ccttaactat cgcagaaaac tcactgaatt gactaatatc gacaacacta tcacggatcg
     4981 cgaattctac aggagaattt gcagccatcc cacttgctat atcaggagct tcaccggaac
     5041 acaccgcaag cattaacgca tttactgtac cagcattaac ctgtgaagaa ggtaatacat
     5101 ttaaattaat actaatcccc gttttcgatg taaaatcttc atccgctaaa gatttcatga
     5161 tttcagccca ttcttttcca ttggacaccc aaatatcaag tgttgttctc tcttttgatg
     5221 tatttttacc tcctacacta tcataatctt ttataaaaga acgtacaaaa ttctgccatg
     5281 ttttccgcac tttctgtaga atattggact gaacaatatc ccatttcttg tcgtatttgc
     5341 ctatgttaac ataatcaatg attaaaggct gatcctgaaa gcataaaagc catgacccaa
     5401 gaattgtttg tgtattgctt aaatcattta atttgcgcgt tatactgtct gggtcagcaa
     5461 tcatttttct caactgatct tttattagaa gtaagtcatt aataaatgta ggtttatcag
     5521 tggaaattga atttagaata tcaatcttgc tttgcatact ttccataagt atttcaaggt
     5581 tatctgttaa atgaggtata tttctctcta attcatattc aaaattgtaa tcagggttat
     5641 tacccgtaag cataataatt tgacgtaaaa tatctgacag cattaataaa tctaccttca
     5701 acgaatcagc aatttctgct ataggaccta attttactgt catcgttaac ttatgttcac
     5761 cttttttaaa ataaaaaagg tatggctcat catctttatc atgcaatatt tccattttcc
     5821 agttcttgct ataatcgaat ttataaagca acatatcttc aaaaggtatg caatcatcaa
     5881 ttgcaatttg tctgtataca ggtattcctt tactccacca ctgcgcagct ctgaacccta
     5941 tattatatag accatcctcc ggaatattaa attcccatgt aatggtatca ttcccttttc
     6001 tccatgacca cccacccatt gcattaagtt tccgggtatc gttataaaca ggttctgtta
     6061 atggatctcc atccgttacc attttaatcg tttgattact tttccctaaa acagcttctt
     6121 ctgcttgaaa ctttattgtt ttatctacat aattatatcc atttttttca tatgaattct
     6181 taacttcgtc atatgtttta attctttcag gagattttat tatcatattt ccaaatacca
     6241 cagattgatt aataaaactc attctaattg tgtgttttcc ttctgaaaag taaaatatta
     6301 gtggctgtgc atacttacct tcactatcta ctacagcagt agtcctccat tccaatacct
     6361 gctcctgtct gggcaatact tcatcaccta tattgtttac agtaggcttt ccggtatact
     6421 cccatattaa agggaatcta atattataca tttcgctaaa agcaacatca ttgtctattt
     6481 taatagaacg atatacatca gagcatgaac ccgctattgt gtagtactcc attaatattt
     6541 catatagccc ctcatgagtt atatctacag accattcaaa ccaatcatat gtgtctgtcc
     6601 acaataatac cggttcctgc cttccgccaa tattagccgt aagagggata tctcccgtgc
     6661 ttgaagccac tattgaatcc atatcaagaa taatttctat attctgagtt ggcatatatc
     6721 cttttttttc ataaagtttt tgaataagag aataactggg ttcactgtct aaatattcgt
     6781 ctatttcctt atatatattt tcagttttag ccgtgaattt ttcttttaac ttcgtaccag
     6841 gctcaccact tacatatgag ctaaatgtaa gtatgctaag aactaaaata atgcatatac
     6901 gcttataaag attattacct gaaaaccacc ttttttcaac cactatttag cccattcctt
     6961 tcatattaat ccattattta tcatcattta taaataataa ttttaacata ttcattttat
     7021 tgcattccta ccgtgggtta cctaatatta tatcatgaat ttcgaagaaa ttgcatgata
     7081 taatcgtgca tgtgcgtttt ttgcgaagca aaaatattcg cacgcacata aattccgccg
     7141 gaggcatata atatcctcat ttgtgaggat attatatcat gaatttgcca aaattcataa
     7201 agatattcta tcacagtagg aatgcaatac ataattattt cataattact acccgtcacc
     7261 acttatttag cagctctgtc ctgtcttgat tggtaaatac ttaatacttt atccaaaccc
     7321 attcctttta cttgagctac ataatcatca tatttatcta atggttcttg acctgtcata
     7381 aactttacct tcatgtcttg aatatattta ttcatatccg gccaaatcaa attcaaatta
     7441 tctgtttctt cttttgtaaa acggattcgt aatagtgatg gtttattgaa atatttttgt
     7501 tgagcatcat ttatatccat tacccatgtg caatatttcg ttttccaggc ttcataatcc
     7561 tgagtatgcg ggaaagaagg ctgcccacct cctacagatt gcaggaactc gttaaaaggt
     7621 actccattcg gtgattctct tacaactatt tttccattaa ctttatcata ggtaattccc
     7681 tcaacaccat aattctgaag gattatagct tcaggatttc tgctgagaaa gtctatatat
     7741 cttactgcaa tatctttatt cttacatttg cctgaaacag ccacaccact tgaaatacta
     7801 ctgcttcctg caataaaata ttgatccccg tacggtcctc ttaaaggatc aagaggaaca
     7861 aacaccggtt cattaccggt tgtgtcgttt gtttctggag aaagtccgct cataatacct
     7921 gcaaatgtac tgtaagtaat tgtacacccc attatatttg actttgcata ttcataagtt
     7981 tctactgttt ttagagcttc ttttttgtgc cataaatctt ctttatacag cttatttata
     8041 tatgccatat attctttata tcttgggttt acgaagtcac atgttacttt tccgtttttg
     8101 tcaagctgga atccaccgac actcagctca agaccaaaac tgttgcctaa agatccatcg
     8161 gcggaatttg aagccatcca tccacataaa ggtaattcgt cattctgacc attgttatta
     8221 agatcatttg tcttaaacaa tttcaatatg ttgtaaaaat cgtccagtgt tttaggtatc
     8281 tcttgattta atctttctac ccactttaca ttaagcaatg gggtaacagc attagcattt
     8341 ataattgaat aaccagctaa gaaataaaaa tgcccatcat cagatgtcct taattttatt
     8401 tcagaagggt tctccttaac atatttcatc aaatatggag cgtttttttc caccagatca
     8461 tcaagctgta cgataactcc atcttctaca aattgcggtg catcagcaag agaaacgtaa
     8521 aacatatcag ggatgtcagt tcctgctgct attctgctct taatggttgc atcataatct
     8581 ttcatgataa cttcccattc aacctcaaca ttggttatct cttcatactt tttgtaaaca
     8641 ggtaattcta tacttgcagg cggtatagta gttgaacctg gtgccggtac tgcaaatacc
     8701 agtttaacct tttcttttgt tagcggaagc tgccccgtat aaacttgttc tttctccgta
     8761 ttacttgcag tgtttgcagg atctttacct gccgttgtaa tatcaaccct tccgtcacta
     8821 tcgtttgctt ttttaactgt acatgctgaa attgtaaata tcatggctac tgctaacaaa
     8881 agactaataa tttttttcat aaattattcc tccctcttgt aattataata cataataaaa
     8941 caaacttatt aaatatatag ttctagcccc ccataatttt tcacctcgca tttctacatt
     9001 acattcttga ttttttaaaa aatgaaatgt gtattatata tcgctgctat gctaaaaaag
     9061 tagtacaaaa tttgctgcga aaaattatat tttatcaata agtacaatgt gacaattact
     9121 gtgcatttgt acaatactct gcgaacactt gtagtttcta atattagtag ttattaaaag
     9181 tatattggta tattttagca tattagtata ttaatgatac gggcgttcaa tccaaattct
     9241 ttttgcattt tcaattgctt cctgacttgg cgtaattttc cctttaatgc tgctatatgg
     9301 ttgtccagga gcataatcct ccggatatgg agaccaattg tcattttcat atcttttcct
     9361 tacattctca tctctaaagt ccggtatctc gaaaggtgct ccattggcaa gtgctgattt
     9421 gtatgcctga atccctacaa gtgtcatatc taagccttta taaacatcat ggtatggctg
     9481 cttgtttgtt tttattgcct cagcaaaatt atatagaaca aaaaagtctg cgccgttatg
     9541 agatgatgat ggtgctatat cattaaaatc agggaaatta ggcaagtatg ttttttcaac
     9601 ctcttcatca ggtttcaagt ccattgaatt atgttttatt ctaaccattt gcttattacc
     9661 ggtacgcatg ttttccataa atccacgggt tccatgaact ctaaaccaat ttccatctcc
     9721 ccgtaatgaa acaccattta acacaacaac agcatcgtta ttcatacgac acataatagt
     9781 tgcagctata tcatgtatgc gaacatgcaa tttttgttca tctttttctg aaaatggaat
     9841 acataatgca tttacacttg taggtcttgt gtctgtaata tacataagag gtccaattga
     9901 atgagtacaa taatatgtag caggttttac atttctccag tgatccaatc ccattgcaag
     9961 ttgattaaaa ccgtctgcag aacacgggtg tatatattca ccttcaccaa attgaatttc
    10021 accaactgca tcttccttat acagttttct catttcctgg atatatcttg tatatggata
    10081 attttcgcca aacaaatata tcttattact tttctcaacg gctctggcaa ggctgactcc
    10141 ttctcccaat gtcttgcatg ctccaacttc actcattaca tgtttttctg cctgtaatgc
    10201 tttaattgca aatggtgcat gttcatgaaa ataattgcat aatattacgg catccatatc
    10261 atgttcaagg aatttcgcat aatcagtata tcccttaact ttatattgat tactaatttc
    10321 atttaataat ttttcttcga tatcacatat ggctgctaat tccatattaa atatgctact
    10381 atgtttattt atatctttta tatactctaa acctcttttt ataccaacta caccaacacg
    10441 aatattctta ttttgattca tacttaacca ccttcctact gccacataga cacagaattt
    10501 gtttttgtat ataaaaacat ttttattatt agcaattatt actcagtatt aaataatagt
    10561 caacacaacg aatttagtaa tttagtaatt tagtaatgtt ttagcaatgc atgctttggt
    10621 ttatagtttt tcgcacttat gctttttctt ctgttttgtt ggagtattta tattttccgt
    10681 taacgtatac gttatatttt atctgtattt attatattat atatagcata cattgtcaat
    10741 attcttttcc gaaaaaacct agtattataa attgtaactt taaatgccgt acatttaatg
    10801 gaaaccctgt ataacgttgc ataatgtttg atgtttagta ctcactccgc tggtgtcaag
    10861 gactaactta gtgaattcat cttaaccatt gacatctagt taggctgttt actttttgca
    10921 aaatcactaa taatgctatc ctcatactcg ctctcatact tgctctcatt tttattactc
    10981 atttttatta tacttattct aatttcggta acgttgccga aatagagcta atattttgtt
    11041 taaatttgta actgttaaat aattataaga gcaattcatc atacattcat atatttactc
    11101 tgccaaattg tatatcagta gtttattctc aggatccttc actgcaatat catatagcat
    11161 tgctgcaatt gaaacatcaa ggcatcccat ccctacaata cttataacaa tacgttcttt
    11221 ctcactttcc ctgccattaa gacttccagt gatgatgttg gggaattcac cataaaagtc
    11281 gttttccttc attctcccct cttcaaaaag ctttacaaac tcgcctcgat gcagatgctg
    11341 ctcaatatga tccacaataa ccttatcagc tttaaaaaca agctcttcat caagttcctg
    11401 gtaagaacct atagttgaga caagtgctcc cggcttaacc caatcattca ttaccagcgg
    11461 tgcatctgca gttgttgcag taacaataat gtcagcatcc cttacagctt cttcagtact
    11521 attatatgct ttaatttcac atggcactgt tttttcagct tttgctataa aatttttaca
    11581 tgcttcagaa taaatatcgc aaattctgac ttccttgaat ttaaacaatt cacaaagaca
    11641 aacaagtgtt gtatatccct gaagacccgc accaataatt gctactatat ctgtgttttt
    11701 gggagccaga tattttgcaa acactgcagt ttgagcaccg gtacgcatat ccgatatcca
    11761 atcgccatca actaatgctt tcatcagtcc tgttctgggg tcagtaagca tgatttttgc
    11821 ttttataaag ggtaagccta ttttatggtt attaatgaaa cctcctaccc actttattcc
    11881 agctatatca caaggctgga catatgaagg catggaattg atccatcctg gaacattgag
    11941 agaagccata tccagggtta tcttggaggg cataataatt ttatcctctg cataccactt
    12001 atacgtttgt tcaactctat caataacact tgaaacagac aaatattttt ctacctgaga
    12061 tttgcttatt atcgctattt tttcttccat aatattacct ccacgtaaca ttttgacttt
    12121 catattttat tcgctttaag ggcacaaata ttttcagcta ttttcacttc acttatcaat
    12181 agtcacgtta tcactatact aaattatcta catctttata tccaattcct gaccatacct
    12241 cttttgcaag tttctttgct tcttcacttg gttctatttt tcccagaatg cttggcaaag
    12301 gctgtcccgg gcctgcatct tcagggaaag gcgaccattt gtcattctca tattttttcc
    12361 tttcacattc acttgtaaag tcaggtacat caaaaggaac accatcctgc agagcacttt
    12421 tccatgctaa tattcctact gttgacattg ttactcctct gtatacatca agataaggct
    12481 gttgaccagt tcttatggct tctgcaaagt agaaattagt ccagaaatcc cctcccccat
    12541 gtcctgcttt ctgagcaagt tcaccatgtt ccggccattc cggtatatat tctttttcaa
    12601 ttacctttcc tggttcaaga tcccactcct catgccatat ccttatttta tcggatgcat
    12661 agtgggcaga gcttcttatt ctttccatag ctcccttact gccatgcagc ctgtaccaag
    12721 tactgtggcc cgggagagaa agaccaaaca gtctgaatac agccccatta tccatccggc
    12781 acaatattac gcccccggga tcccttaccc tcattgtttc cttgtcaatc tctctgcaag
    12841 ctattgatag accatttact ttctttggca tggtatctgt aatatacata agcggagcca
    12901 gggcatgagt attataatat gttggtggta tatggtttct ccagtgattt ttacccggtg
    12961 aaattctaag tctgcttctt gcatccattg gatggttata ttctccgtca gcatatgtta
    13021 cctcaccaat ttctccctga cggtaaagct tcctcatttc ttgattgaat ttcatataag
    13081 gataattctc tgcaagcatg taaacaagac ctgacttttc aacagccctg cacaaagcaa
    13141 caccatctgc aatggttgca ttgcagcttg tctcacttat aacatgcttg cctgcttcca
    13201 aagcttttaa tgcgaaaggg gcatgttcat taaagaagtt tgcaagtact acagcatcca
    13261 tgtcatgttc aaggaatttg tcatactcag tatatgttgg cacatttaat tcttctccaa
    13321 ccttttttag gattttatct aatccttttt caaaaagata gtt
//
LOCUS       DAOSDD010000114        13183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1828_length_13183_cov_4.638559, whole genome shotgun sequence.
ACCESSION   DAOSDD010000114 DAOSDD010000000
VERSION     DAOSDD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_13183_cov_4.638559"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="PK733_11005"
     CDS             <1..1842
                     /locus_tag="PK733_11005"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ11106.1"
                     /translation="ARINNGISLNYDFTSLGIGLTLEIKLDGDSMTVCIPADKIQESE
                     NKGIKSIELMPFFGAADHTVDGYLLYPDGCGAITKYANVDQRSKNVKAFTWDIYGAEQ
                     VDFNECKQMEKDQKYQAMLPIYGIKNGDNAILAAVEKGAEDTSIKVSPDGFIVNLNRA
                     SFAFNYRHFYKINLSNIVVNGASVLRNTMGTRVDKEMIKTDREVRIFFLSGETANYSG
                     MANTYREYLIKSNLLKKVIIENDNIPLALELFMGIKENRLLFDKYVTMTTFKNAISIS
                     EELKNAGVDSMQVMLKGWTKGGYGLYPVNWPPEKKLGGKKGLREFSDYARKNNMQIFM
                     RNNFIQADSKNRGFSTRNDVVMQGSNLPVTGMGKTEFLFNPFFALQRFVSFFNEFSRW
                     CSEGVALERIGINIYHDYNKANPAGRAETIEKWEDMLDTVIRNQRPAAVEGGNQYVLK
                     YADRLCDIPVRSSQYNITDEEVPFYQMVVHGMIPYTSEAANLSYDLTMHKLKLVEYGC
                     MPYFELTYSKSTNLNNTEYNKLFTSYYINWYKQAADIYNEFNERLKCVWSQKIIEHDK
                     MEENTYRIRYSNGITVYVNYSKNDIECDGYSIKGKDYIVVGKGGVIN"
     gene            1839..2744
                     /locus_tag="PK733_11010"
     CDS             1839..2744
                     /locus_tag="PK733_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ11107.1"
                     /translation="MKKLKLSMEKRNMVEGYIFISPFLLGTLIFFAFPLFMSIKLSFG
                     KILKMEGFKIKWIGIDNYVRAFLVDINFVPMFINVVKQTFIKVLLIIIFSLLLSILIN
                     KNIKFRGFFRTVFFIPFLLGTGDVMKQLLGQGVDKQVISLADGTLIPRGMLAYLGTNV
                     VTVIDVLFSIIVVVLWSSGVQILLFLSGLQTIPISLYESSKVDGATEWEMFWKITLPM
                     ISPIMLLNIIYTLVDSFTDVTNPILSYIQNTAFINLNFEYAAAMGWIYLLFIFILVLL
                     VMRVLKSYTHMDDVKGVNKNAKRND"
     gene            2725..3720
                     /locus_tag="PK733_11015"
     CDS             2725..3720
                     /locus_tag="PK733_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11108.1"
                     /translation="MQKGTTDVSIERKVIYKSGNVVLRNVMEWVKKPQKTGEKVAKLA
                     TYVLLFDLAFVFLFPFLYMIVESLKTNADLYDLTVNWVPRTLKFQNYVIAFRVLEYTK
                     YIKNSLVITIMATIGHVLACSFVGYGFARHDFPGKNWLFILVIVALIVPVQTVIVPMY
                     MLYANLKWLNTYLPVILPTFFGYGLKGALFIFIFRQFYLGLPKELENAAKIDGCGFLR
                     TYWNIVLPVARSAFMVAVVLSLVWHWNDFYEPAIYVTKPVLTILPARLNSLVTLVNNP
                     PENLFAEMSIGDGEDTLNNAVLMAGTFMIVLPVIISFAFLQKKFIQGIERTGLVE"
     gene            complement(3816..6485)
                     /locus_tag="PK733_11020"
     CDS             complement(3816..6485)
                     /locus_tag="PK733_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOJ11109.1"
                     /translation="MYLGSIAQKESGIAGSYTYMRRGTCIPSFPDLYPDLYSEPHKNL
                     HLDLLKDSSLENSASNECIPFEKGSLFNKETIGWKWLTVLDIGVDIVIKLDKPCFIDR
                     IAIHQGSESAVKSVRALCSDTDGVYACKGRVDAATSEMLESNHLEVTLGVEAQNIIIR
                     LDACFKDIVIEGLDIIGAVFDDYMVYPVPASINMSKGTAMHISNIEHIVIGKESSDDT
                     AFAANLLKEKLHENYSLSLPIVYSEELQSHSNSIVLGKCNEIAMLDSGINSAPSAEGY
                     IINACEGAVYLQGADRRGLIYGVEALLMLINDKEIPACIVNDYPFMGIRGVHFGLPPR
                     EEIPFIKRLIRYVFAPMRMNTIFLEFAGGMRYDRHPEINKVWEEGNKKAKDGKWPPFP
                     HGDMVSGGSVLEKQEVADLVDYARSYGIEVIPEVQSLGHVQYITKAYPEVAETEYEDN
                     NTDKDKADIDLKKEDAPPDKFYKDSYCPSNEKSYSIIFDLIDEIVEVVRPTEYVHMGH
                     DEVYTMGTCPLCKDKDHADLYASDVNKLYNYLASKNLKMMIWGDMLHNVTKYKTPPAI
                     DKIPKDIVLLDFIWYFHLDKDIEEHLLDHGFKVIMGNMYSSHYPRFEKRARENGIIGA
                     EVSTWKRMDEYNMGFEGKIYDFIYSANMMWSSSYRSDLRLSYDFIISRLMPRIRSQIH
                     GEAYPSLSETKRCLPVVLPEQQSTAPIPWELSTILGKQGGHTLNGLPFNIGAPQVVLT
                     KTSSMGQSYETLTIPVNHAFDSLIFLHAAGENVERIPWKNPYQIGSYIVEYEDGTRLE
                     VPIEYGANIGVWYKRYGEPLKHAYYRHQGYVSTYFADAYIQSKTSSGKDVTVYGYEWI
                     NPYKDKTIVSVELAAKGDTDASVMLFAVTGIKR"
     gene            complement(6622..8250)
                     /locus_tag="PK733_11025"
     CDS             complement(6622..8250)
                     /locus_tag="PK733_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11110.1"
                     /translation="MKKKVVVIFLVFSMILSFTIVGCRKDNKTNINDESKNSASSQTD
                     NINKTGLPIVKDKVTLKMMAAKWHYQKNFADIETFRELEAKTNVSISWDLIPNESYVE
                     KKNLLLASDDLPDAFFGTSAGGLSLDDIMKYGPQGVLIPLEKLIDDYSPNITSLFAKK
                     PDVLRVSKSPDSHIYTLPFVQELESNTVFSRMYINKTWVKKLGFEIPTSTDELYKVLK
                     AFKEQDPNGNKKPDEIPMSFRYKNHNYGHYGLFGAFGSLENTNHVSVQDNKVIITPTT
                     NEYKNAILYFNKLYKEGLIDQEAFTQDGSQYSAKGSGSDMIYGLYFGWGGAEIVGNER
                     YEENYIPFAQVKGPEGHQMWLSRSPAFWRGYFAITSKNKYPEITMRWADELYNETVSI
                     QLQYGPLGVNLKQRDDGKIEYLPTPSDKSYGEFRLHAAPGIGPFAITAETFANKIIPN
                     DNMEVDNAELEVYKPYIGNNYLPDVIFTAEQTAKLANLKTDIFEYMNTMQARWITGDG
                     SIEKEWDSYISELKKMGLDDLQKVYQDAYDIYMK"
     gene            complement(8466..9359)
                     /locus_tag="PK733_11030"
     CDS             complement(8466..9359)
                     /locus_tag="PK733_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11111.1"
                     /translation="MVKTRSPLHDKFFDTINYIILTLILLIVIYPLYFIVIASISNPN
                     FVANGEVWLLPKGINFEGYKSIFKDPIIGIGYKNTLIYTCLGTIINVIITLSAAYALS
                     RKDLAGRNLLMFVFIFTMFFSGGMIPTYLIVKKLHLIGTIWAMVIPNALSVYYLIISR
                     TFFQSTIPDELLEAALISGCSNMRFYTSIVLPLSKALIAVMILFYGVAHWNSFFNALL
                     YLNNEEMYPLQLILRNILLANMMREKMHDALVDSVNQVQNKADMIKFGIIIVASLPVL
                     IIYPFIQKFFIKGVMIGAIKG"
     gene            complement(9380..10357)
                     /locus_tag="PK733_11035"
     CDS             complement(9380..10357)
                     /locus_tag="PK733_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11112.1"
                     /translation="MKGMAIQKKRAQTPVSISKENKKRLKRLLKNTLKNYQLYLLVLP
                     ALVYIIIFHYGAMYGLQIAFKDYFVTKGISGSPWVGFVHFKRFFNSAQFSVVIKNTVF
                     LSLYGLLLGFPIPILLALSINNVQNKFFKSLVQTVTYAPHFISTVVIVSMLLIFLSPR
                     SGFVNIVIQSFGGTPVHFIAKPEWFKSLYVFSDIWQSSGWSAIIYIAALSNISFELHE
                     AAIIDGANKFQRTKYIDIPGIMPTVITLLIINIGNLMSVGFEKAFLMQNSLNVMSSEI
                     IQTYVYKIGLVSADFSYSAAIGLFNSAINFLLLIGTNRLSKKLTDISLW"
     gene            complement(10672..12993)
                     /locus_tag="PK733_11040"
     CDS             complement(10672..12993)
                     /locus_tag="PK733_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11113.1"
                     /translation="MSPLKNKILFQSIFAKYLVSYFIILLIPIVFTLLFSYNYFINIF
                     KNEIIINNSNMLEYIRNKIDDEIYQFKIISNEIQNNSKLTSYAINKNTISKMSAINEL
                     KNYSIRSDLIEKLVLYIYDDRYLYTSSGTYTINSFIDVYKYNGWSYEEFCSILNSNSN
                     SGLISENISHSEIKADNYTPGYIAYTQQLPSNSMYPSMSILFLLREDTIKNSISDVIK
                     MHNANTLILDNNYKIITCTQNTDYLSKINIGSILQNKTGNSGIITIDKVKYYYLYMKS
                     EITKWTFLTLIPEKDAMQYVFDVRKRSIYVMISIFLFGSLIIYLLVNYNYNPIKQLKE
                     FADRTWDKSQDTVQHKTSDAGYSNEIEAIKSIIGQMQKSDENLRIKIKDSVPAVKEFL
                     LKTLLQGRFSSIEEFNNTAEEANLAFTKPCFRVILISTEKYNTVADKIPKNDIIKYIE
                     NYFIDFAEVYGIDNFEGSNLIFIMASDKIKSASFKETLVLLQKAINDIWGLHITIGAG
                     GLHISHENIPQSYIEASVALDYSFVLGKNKIIFFDEINNSQKDIDYPINELNDLPFYM
                     KQGDTGKIVKSLESIILNINRNNAPLFMVRCVCYDIINTVIKTMNQLDIGFKSTSNDY
                     LDAFSSCDTADKLIGIIKELCIDICDIISMSKESCNSNLRERVLEYLSTNCEDPNFSV
                     RVMAQDLDVSQSYLSRFFKDQIGITITDYIMLQRIEKAKYLLKTTDEPLRNIITKIGY
                     FSEASFVRMFKRVEGMTPGEYRKLTFSPEKSGK"
BASE COUNT         4193 a         2255 c         2286 g         4449 t
ORIGIN      
        1 gcacgtataa acaatggaat atctttaaac tatgacttta cctctctggg tatagggctg
       61 accctggaaa taaagcttga cggggacagt atgacagtgt gtatacctgc agataaaata
      121 caggaaagcg aaaataaggg aattaaaagt attgaactta tgccgttttt tggtgcagca
      181 gatcatacag tagatgggta tctgctttat cctgatggat gtggtgctat aacaaaatat
      241 gccaatgtag atcagaggtc aaaaaatgtt aaggcattca cttgggatat atatggtgct
      301 gaacaagttg attttaatga atgcaagcaa atggagaagg atcagaaata ccaggccatg
      361 cttccaatat acggaataaa gaatggtgat aatgccatcc ttgctgcagt tgaaaaggga
      421 gcggaagaca caagtattaa ggttagtcct gatggtttta tagtaaatct taatcgtgca
      481 agctttgcat ttaactacag gcacttttac aagataaacc tgtcaaacat tgtagtaaat
      541 ggggcaagtg tattaagaaa cactatggga accagggtag acaaggaaat gataaagaca
      601 gacagagaag tgcgtatttt ctttctgagt ggggaaactg caaattatag cgggatggcg
      661 aatacctaca gggaatattt gattaaaagc aatctcctga agaaagtaat aatagagaac
      721 gataatatac cgttggctct tgaattattc atgggtataa aggaaaacag gcttcttttt
      781 gataaatacg ttacaatgac tacctttaaa aatgccatat ccataagtga agagcttaaa
      841 aatgccggag ttgatagtat gcaggttatg cttaagggct ggacaaaagg gggctatggc
      901 ttatatccgg ttaactggcc gcctgagaaa aagctaggag gaaagaaagg acttagagaa
      961 ttttctgatt atgctcggaa aaataacatg cagattttta tgagaaataa ctttatacaa
     1021 gcagattcca aaaatagggg attttcaacc agaaatgatg tagtaatgca gggcagcaat
     1081 ctaccggtaa caggtatggg aaagacagaa tttcttttta atccattttt tgcattgcaa
     1141 agatttgtta gcttctttaa tgaattttca agatggtgca gtgaaggagt tgctcttgaa
     1201 agaattggca ttaatatata ccatgactac aacaaggcaa atcctgccgg cagagcggag
     1261 accatagaaa agtgggaaga tatgctggat actgtaataa gaaatcaaag gccggcagca
     1321 gtagaaggcg gaaatcagta tgtattaaaa tatgcagaca ggttgtgtga catacctgta
     1381 agaagctctc aatacaatat aactgatgag gaggtaccgt tctaccagat ggtagttcat
     1441 ggaatgatac catatacatc agaggctgca aatttatcgt atgatcttac aatgcataag
     1501 ttaaaattgg tagaatatgg ctgcatgcca tattttgaac tgacatatag caaatctaca
     1561 aatctaaaca acacagaata taacaagctg tttacttctt attacattaa ctggtataag
     1621 caggctgcag atatctataa cgaattcaat gaaaggctta aatgcgtgtg gagccagaag
     1681 ataatagaac acgataaaat ggaagaaaac acatatcgta ttagatatag caacggaatt
     1741 acggtatacg taaattatag taaaaacgat atagaatgtg atggctatag tataaagggt
     1801 aaagactata ttgtagtagg aaaaggggga gtgattaatt gaaaaagtta aaactcagta
     1861 tggagaaaag gaatatggtt gagggataca tatttatatc acctttttta cttggaacac
     1921 tgattttttt tgcatttcct cttttcatgt ccataaagct cagctttgga aagatattga
     1981 agatggaagg ttttaaaatt aaatggattg ggattgacaa ctatgtacgg gccttcctgg
     2041 tagatattaa ctttgtgcca atgtttataa atgtggtaaa gcagacattt atcaaagtat
     2101 tacttatcat tatattctcg ctgcttttgt ccatactaat aaataaaaac ataaaattca
     2161 gaggtttttt caggactgta ttcttcattc cttttctgct tggtacggga gatgtaatga
     2221 agcagctact gggacagggg gttgataaac aggtaatctc tcttgcagat ggtacattaa
     2281 ttcccagggg gatgcttgcg taccttggaa caaatgttgt aacagtcata gatgtattat
     2341 tcagcattat tgtagttgtc ctttggagct caggagtaca aattcttcta tttttgtccg
     2401 ggcttcagac aatacccata tcgctttatg aatcatctaa agttgatgga gctacagaat
     2461 gggagatgtt ttggaaaata acacttccta tgatatcgcc gataatgctt ttaaatataa
     2521 tatacacact ggtagactca tttacagatg tgactaaccc catattaagc tatattcaga
     2581 acactgcgtt tattaacctg aatttcgaat atgctgcggc aatgggatgg atatacctgt
     2641 tatttatttt tatccttgtt ctgcttgtaa tgagggtgct caaaagttat acccatatgg
     2701 atgatgtgaa gggagtgaat aaaaatgcaa aaaggaacga ctgatgtatc catagaaaga
     2761 aaagtaatat acaaaagtgg caatgttgta ctacgtaatg ttatggaatg ggtgaaaaag
     2821 cctcaaaaaa caggtgaaaa agtagcaaaa ctggctacat atgtgctctt gtttgacctt
     2881 gcttttgtat tcttatttcc gttcctttat atgatagtgg aatcactgaa gacaaatgca
     2941 gatctatatg accttacagt aaactgggta cccagaacat tgaaattcca aaactatgtg
     3001 attgctttta gagtgcttga atataccaaa tatataaaaa actccttggt aattacaatt
     3061 atggcaacca tagggcatgt attggcctgc tcatttgtag gctatggatt tgcgagacat
     3121 gactttcccg gaaagaactg gctgttcatc ctggttattg ttgcactgat agtaccggtg
     3181 cagaccgtta ttgtacctat gtacatgcta tatgcaaacc ttaaatggct taacacctat
     3241 cttccggtga ttctgcctac gtttttcgga tatggcctta agggtgcgct gtttatattt
     3301 atattccggc agttttacct tggacttccg aaagaactgg agaatgcagc aaagattgat
     3361 gggtgcggct tcttgagaac ttactggaat attgtgctac ctgtagcaag atccgctttc
     3421 atggtagcag ttgttttatc tctggtgtgg cactggaatg acttctatga acctgctata
     3481 tatgtaacaa agcctgtact gaccatttta cctgcaaggc taaattcact ggtaactctg
     3541 gtaaataatc cgcccgagaa tctgtttgct gagatgagta taggggatgg agaggataca
     3601 ttgaataatg ctgtattaat ggcagggaca tttatgattg tattaccggt aataataagt
     3661 ttcgcatttc tgcagaaaaa attcatacag ggaattgaac gtacgggttt ggtagaataa
     3721 tttataatta aggtaaaagc gagggggaaa taaaacaaaa ttccccctgc tgcagtacct
     3781 ctaaatttga aaatgcacct aataaaataa accttttatc ttttaattcc tgtaaccgca
     3841 aaaagcatta ccgatgcatc agtatctccc tttgcagcaa gttccacaga aacaatagtc
     3901 ttatccttat atggattaat ccattcatat ccatacacag taacatcttt acctgatgat
     3961 gtttttgatt gtatatatgc atcagcaaag taagttgaaa catagccctg atggcgataa
     4021 taagcatgtt ttaaaggttc accatatctt ttgtaccata ctccaatatt ggcaccatat
     4081 tcaatgggta cttccagtct ggtaccgtct tcatattcta cgatatatga accaatttga
     4141 tagggatttt tccacggaat gcgctcaaca ttttctcctg cagcatgcag aaagattaaa
     4201 ctatcaaaag catgattaac aggaatagtt aaagtctcat aactttgacc catacttgat
     4261 gtttttgtca gtacaacctg tggtgctcca atgttaaatg gcagtccatt taatgtatgt
     4321 ccaccttgct ttcccaaaat tgttgagagt tcccatggaa ttggagccgt gctttgctgt
     4381 tcagggagaa caacaggtaa acatctcttt gtttcactaa gagatggata tgcttctcca
     4441 tgaatttgtg aacgtattcg aggcatcagc cgtgatataa taaagtcata gctaaggcgc
     4501 aggtcacttc tgtaagaaga ggaccacatc atgttggctg aatagataaa atcatagatt
     4561 ttgccttcaa aacccatatt atattcgtcc atacgcttcc atgtagatac ttccgcccca
     4621 atgataccat tctcccttgc acgtttttca aagcgcggat agtggctgga gtacatattg
     4681 cccataatta ctttaaaacc atgatccagc agatgctctt caatatcttt atcaagatga
     4741 aaataccaga taaagtccaa taggactatg tctttaggta ttttatcaat tgcaggtgga
     4801 gttttatatt tagttacatt atggagcatg tcgccccaga tcatcatttt taaattcttg
     4861 ctagcaagat agttatatag cttattaaca tcagatgcat acaaatctgc atgatcctta
     4921 tccttacata gaggacaagt gcccattgta taaacctcat catgccccat gtgtacatat
     4981 tcggttgggc ggacaacctc aacaatttca tcgattaaat cgaatatgat tgaatagctc
     5041 ttttcattgg aaggacagta gctgtcttta taaaatttgt ccggcggtgc atcttctttc
     5101 ttaaggtcaa tatcagcttt atccttatct gtattattat cttcatattc agtctctgct
     5161 acttccggat acgcctttgt aatatattga acatgcccta aagactgtac ttccgggatt
     5221 acttcaatac cgtaggaccg ggcatagtcc accagatcgg caacttcctg tttctcaaga
     5281 accgatccac ccgaaaccat atctccatgt ggaaatggcg gccatttccc gtctttagcc
     5341 tttttattgc cttcttccca aactttattt atttcaggat gcctatcata gcgcatacca
     5401 cctgcaaatt caagaaaaat tgtgttcatt cgcattggcg caaaaacata gcgtatcaac
     5461 cgcttgataa aaggtatttc ttcacgggga ggaagtccaa aatgtacacc gcggattccc
     5521 ataaatggat aatcatttac tatgcatgca ggaatttcct tatcattaat taacataagc
     5581 aatgcttcga caccgtatat aaggcctctt ctgtctgccc cctgcagata tactgcacct
     5641 tcacatgcat tgataatata gccttctgca gatggtgcac tgtttatgcc tgaatcaagc
     5701 atagcaattt cattacattt accgagaaca attgagttgg aatgtgactg caattcttct
     5761 gagtatacta tcggcaatga aagagaataa ttttcatgta atttttcttt taaaagattt
     5821 gcagcaaatg cagtgtcatc tgatgactct ttcccgatta caatgtgttc aatattagat
     5881 atgtgcattg cagtaccctt gctcatatta atagaagcag gtacagggta caccatatag
     5941 tcgtcaaata cagctcctat aatgtcaagc ccttcaataa caatatcttt aaagcatgcg
     6001 tcaagtctga tgattatatt ttgcgcttca acacccaagg ttacttcaag atgattgctt
     6061 tccagcattt cgctagtcgc tgcatcaacc cttcctttgc aggcatatac accatcggtg
     6121 tcagaacata gtgctcttac cgatttgaca gctgattcgc tgccctgatg aattgcaata
     6181 cgatcaataa aacatggctt atcaagttta attactatat ctacacctat atcaagaact
     6241 gtaagccact tccagccaat ggtttcttta ttaaaaaggc ttcctttttc aaatggaatg
     6301 cattcattac ttgcactgtt ttcaagactt gaatctttaa gtaaatctaa atgcaaattt
     6361 ttgtgcggtt ctgaatacaa atccgggtat aagtccggaa aagaaggtat acatgttcca
     6421 cggcgcatat atgtatatga accggcaata cctgattctt tttgtgcaat acttcctaaa
     6481 tacataaaac ctaacctcct tgttcaattg tatatttaca aaaaacccat tacaaaatct
     6541 ttagtattat tgttatatta agcaaaagat ctcgcaatgg gttaattgta ctatataatt
     6601 acattatata tcaacagttt attatttcat atatatgtca tatgcgtctt gataaacttt
     6661 ttgcagatca tcaagtccca ttttcttaag ttctgatata tagctgtccc attctttttc
     6721 gattgagcca tcacctgtaa tccatcttgc ttgcatggta ttcatgtact caaaaatatc
     6781 agtttttaaa tttgcaagct tggcagtttg ttctgctgta aatataacat caggaagata
     6841 gttgttgccg atatatggct tgtacacttc taattctgca ttatctactt ccatgttatc
     6901 gttaggaata attttattgg caaaggtttc tgctgtaatt gcaaatggcc ctataccagg
     6961 ggcagcgtgc aatctgaatt cgccataact cttatcggat ggagtaggaa ggtactctat
     7021 tttaccatca tcacgctgtt tcaaatttac tccaagcgga ccatattgca gttgtattga
     7081 gactgtttcg ttataaagtt catctgccca tctcattgtt atttcaggat acttattctt
     7141 tgatgttata gcaaaataac ctctccaaaa agcaggggac cttgataacc acatttgatg
     7201 gccctcagga ccttttactt gagcaaaggg gatataattt tcttcatatc tttcattgcc
     7261 tactatttca gcgccgcccc atccaaaata tagtccatat atcatgtcac ttcctgagcc
     7321 ttttgcagaa tactgtgagc cgtcttgagt gaaagcttcc tgatctatca gaccttcttt
     7381 gtaaagtttg ttaaaataca ggattgcatt cttatattcg ttagttgttg gtgtaattat
     7441 taccttatta tcttgtacgc tgacatgatt agtattttca aggcttccaa aagctccgaa
     7501 taatccataa tgcccataat tgtgattttt atatctaaat gacataggaa tttcatctgg
     7561 ttttttattt ccgttgggat cttgctcttt aaatgctttt aatactttat atagttcatc
     7621 ggttgatgtt ggaatttcaa agcccaattt cttaacccat gttttattta tatacattct
     7681 tgagaaaaca gtatttgatt ccagttcctg aacaaaaggc agggtatata tatggctgtc
     7741 tggtgatttt gaaacacgta agacatcagg ctttttggca aatagggatg taatatttgg
     7801 agaataatca tcaatgagtt tttcaagagg tataagaacc ccctgcggac cgtatttcat
     7861 aatatcatca agacttaatc cgcctgcact ggttccgaaa aaagcatccg gtagatcatc
     7921 acttgcaaga agcaaatttt tcttttcaac ataggattcg ttaggtatta gatcccagct
     7981 gatagataca tttgttttag cttcaagctc tctaaatgtt tctatatccg caaagttttt
     8041 ctgataatgc cactttgctg ccatcatttt aagggttact ttatctttaa ctataggaag
     8101 ccctgtttta tttatgttat cggtttgact tgatgctgaa tttttacttt catcattgat
     8161 attggtttta ttgtctttcc tgcaaccaac tattgtaaat gatagtatca tagagaaaac
     8221 taaaaaaata actactactt tctttttcat aataaattac ctccatattt catagtttat
     8281 catattaata acatgatata ataaaagtgt gtggggaagc tgtaattcac cagttcataa
     8341 accacaattt tatttaatgg ttgaatttat agcttcttca ttttgaccag acatataatt
     8401 atgtgtatca catctcctcc ttcctaattc agtaaagccg gctgtgacgt ctcacctcct
     8461 tcatgttagc cttttatggc tccaatcatt actcctttaa taaaaaactt ctgaatgaat
     8521 ggatatataa ttaataccgg cagactggct actataatta taccaaattt aatcatatca
     8581 gctttatttt gaacctggtt aacagaatca accaaggcat catgcatttt ttcacgcatc
     8641 atattagcta ataatatgtt tctgagaatt aactgcaagg gatacatttc ttcattgttt
     8701 agatataaaa gtgcattgaa gaatgaattc cagtgagcta caccataaaa gagtatcata
     8761 actgcaatca gtgctttaga taatggcagt acaatggaag tatagaatcg catatttgaa
     8821 caacctgaaa tcaaagctgc ttccagcagt tcatcaggaa tagtggattg gaaaaatgtt
     8881 ctggaaatta taaggtaata tactgataaa gcatttggaa ttaccattgc ccaaatagtg
     8941 ccaataaggt gcagcttttt tactataaga taagtaggta tcattccacc agagaagaac
     9001 attgtaaata taaatacaaa cataagcaga tttctgcctg caaggtcttt ccttgatagt
     9061 gcatacgcag cagatagtgt aataataaca tttataattg taccaagaca agtataaatt
     9121 aatgtgtttt tatatcctat cccaattata ggatctttaa aaatgctttt atacccttca
     9181 aaatttatac ccttcggtaa taaccatact tctccgtttg caacaaaatt aggattactg
     9241 attgatgcga ttacaataaa atatagtgga tatattacaa ttagtaatat taatgtaagt
     9301 ataatataat taatagtatc aaaaaattta tcatgcagtg gacttcttgt ttttaccata
     9361 ttgttatcct ccttttaact taccacaaac tgatatcagt aagttttttt gatagtctgt
     9421 tagttccaat aagaagtaaa aaatttattg cagagttaaa taggcctata gctgcagagt
     9481 aactgaaatc agcacttact aagccaattt tataaacata tgtttgaata atttctgaag
     9541 acataacatt tagggaattc tgcataagaa atgctttttc aaatccaacg ctcataagat
     9601 ttcctatatt tattattaat aatgtaataa ctgttggcat aattcccggt atgtctatgt
     9661 attttgttct ttgaaatttg tttgcaccat ctataattgc ggcttcatgc agctcaaagc
     9721 tgatattgga taaagctgca atatatataa ttgcagacca cccggatgac tgccatatat
     9781 cggaaaagac atataatgat ttaaaccact caggctttgc tataaaatgt acaggtgttc
     9841 ctccgaaaga ttgaataaca atattaacaa aaccgcttct tggcgaaagg aatataagca
     9901 acattgaaac aattactact gttgatataa agtgtggagc ataggtaact gtttgaacaa
     9961 gggatttaaa gaacttgttc tgaacattat ttattgaaag agccagtaaa ataggtatgg
    10021 gaaatcccag cagcaatcca tataaactta agaaaactgt atttttaatg actactgaga
    10081 attgagctga attaaaaaat cttttaaaat gtacaaatcc aacccaaggg ctcccggata
    10141 tgcctttagt tacaaaataa tctttaaatg ctatttgcag accatacatt gccccataat
    10201 gaaatataat tatatatact aaagcaggta atactaacaa atatagctgg tagttcttta
    10261 aagtattttt cagtaacctc ttaagtctct tcttattttc cttagatata gatacaggtg
    10321 tttgcgccct tttcttttgt attgccatcc ccttcattta tgcgtttgcc tccttaatat
    10381 agatttaaat taatattgat ttaaaattat tgctgattta gattcatata atttttatca
    10441 tgaattctta ttaaagtaaa tatgcacttt ataactctgt ttgcatgata ttacaattga
    10501 gaatttgctt tagtatagcg tatttccagg cttacagatt atgtaatgat ttgttgatgt
    10561 aataggaggc atatatagta acaagaggca catatgcatt atattcctgc aattactaat
    10621 atgcatcttg ctttagaatt ataaatttaa acaattaaat attagctggg tttatttacc
    10681 ggatttttct ggactaaagg taagttttct gtattctccg ggtgtcattc cttctactct
    10741 tttaaacatt cttacaaaac ttgcttcgct aaaatagcca atttttgtta taatatttcg
    10801 taaaggttca tctgtagttt ttaacagata ctttgccttt tcaattcttt gaagcattat
    10861 ataatctgta atggttatgc caatttggtc tttgaaaaaa cggctaaggt atgattgtga
    10921 gacatccagg tcttgagcca taactcttac agaaaaattc gggtcttcac agttcgttga
    10981 aaggtattct aaaactcttt ctctaagatt actgttgcag ctttcctttg acatacttat
    11041 aatatcgcat atatcaatac ataattcttt tattatgcca atcaatttat ctgctgtatc
    11101 acaactgctg aatgcatcaa gataatcatt gcttgttgat ttaaagccga tatcaagctg
    11161 gttcatagtt tttataactg tattgataat gtcataacat acacagcgaa ccataaatag
    11221 aggagcatta ttcctattta tattgagaat aatactttcc agtgatttta ctatctttcc
    11281 ggtatcacct tgtttcatat agaatggaag atcattcaat tcattaatgg ggtaatctat
    11341 gtctttctgt gagttgttta tttcatcaaa aaagataatc ttgtttttac ctaacacaaa
    11401 ggaatagtct agtgctacag atgcttcaat ataagattgg ggtatatttt cgtgtgatat
    11461 atgcaagcca cctgctccaa tagtaatatg tagcccccat atatcattta tggctttttg
    11521 cagcagaaca agagtttctt taaacgaagc tgattttatc ttatcagatg ccatgataaa
    11581 aatcaggttg ctgccttcaa aattatcaat gccgtatact tcggcaaagt caatgaaata
    11641 gttttctata tacttaatta tatcattttt cggtatttta tcggcaacag tattatattt
    11701 ttctgtagaa atcaatataa ctctaaagca aggctttgta aaagcaagat ttgcttcttc
    11761 tgctgtatta ttaaactctt ctatactgct gaatctgcct tgaagaagtg tttttaataa
    11821 aaactctttg acagcgggaa cactgtcttt aatttttatt ctcaaatttt catcactttt
    11881 ctgcatctgc ccaataatcg attttattgc ttcaatttca ttgctgtaac cggcatcgct
    11941 ggttttatgc tgaactgtat cctgactttt atcccatgtc cggtcggcaa attcctttag
    12001 ttgttttatg ggattatagt tatagtttac cagaagatat ataattaagc taccaaaaag
    12061 aaatatgctt atcataacat aaatacttct tttccttaca tcaaatacat attgcatagc
    12121 atccttttct ggtattagcg taagaaacgt ccatttggta atttctgatt tcatataaag
    12181 ataataatat ttgactttat caattgtaat aattcccgaa ttgccggttt tattttgcaa
    12241 gatgctgcct atattgattt tagacaaata atccgtattc tgcgtacatg ttattatctt
    12301 ataattatta tcaagaataa gagtatttgc attatgcatt tttatcacat cagatatact
    12361 gtttttaatt gtatcttctc ttagcaaaaa cagtatggac atagaaggat acattgagtt
    12421 tgatggtaat tgctgggtat acgcaatata tccgggagta taattatctg ctttaatttc
    12481 gctatgactt atgttttccg atattaaccc ggagttgctg tttgaattta gaatgctgca
    12541 aaattcttca tagctccagc cattatactt gtaaacatct ataaagctat ttattgtata
    12601 ggtgccggag gaagtataga ggtatctatc atcataaata tatagaacca gcttttctat
    12661 aaggtcgctt cttatgctgt aattcttcaa ttcgttaatt gcactcatct ttgaaattgt
    12721 attcttgttt atagcatatg aggtcaactt gctgttgttc tgaatttcgt ttgaaataat
    12781 tttgaactga taaatttcat cgtcaatttt attccgtata tattccaaca tattagagtt
    12841 gtttattata atttcatttt tgaaaatatt tataaagtaa ttatatgaaa atagcaaagt
    12901 aaaaactatt ggtattaata gtataataaa gtaagataca agatattttg cgaatattga
    12961 ctgaaataaa attttgtttt tcaggggaga cactttatca cttccatatt ctatattatt
    13021 ttgcaccttg tattttaaat attttattat ttaatacaaa taatatatca caaacttttg
    13081 cagatatcaa tttatttaaa taatgcccta taaatgggtg cattttccat atttgaggga
    13141 aaatatggca agaagcgatt tccatgtata atattcactt cag
//
LOCUS       DAOSDD010000115        13135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1836_length_13135_cov_4.550765, whole genome shotgun sequence.
ACCESSION   DAOSDD010000115 DAOSDD010000000
VERSION     DAOSDD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_13135_cov_4.550765"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1242
                     /locus_tag="PK733_11045"
     CDS             418..1242
                     /locus_tag="PK733_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11114.1"
                     /translation="MYYYISFNHQNFISNNEVSFLENNDLSPSKPLFLQIKEAIKEKI
                     LNGEYQYGSKIPSENELCKLFNVSRITVRSAISEAMKEGLLFTVQGKGTFVKDLKDTK
                     ISQALPYINSFESTIKNLGLSAGTEILKVETIPADIEISKILDINIGTNVLILSLLGK
                     ADNEGYVIYNSYFSEIIGNKIYELAKKEVEKGAAFSTLDLYKKLNQPVPAFIEQTFEA
                     VAADASTSKVLNLKKDSPLFLVSSIIYTGNSAPLEYRKAFYRSDKYKFHIKRELSL"
     gene            complement(1370..2269)
                     /locus_tag="PK733_11050"
     CDS             complement(1370..2269)
                     /locus_tag="PK733_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11115.1"
                     /translation="MELKMKKIDIHVHTRAFKGIPRKDAGTTYATPEELIAMYDKLGV
                     EKGVIMTSFCLECGLQGQSNEETMYLVEKYPGKFDWFCNIDPRMGDNTPNYDLSYFIS
                     YYKERGAKGVGEVSYNLYMDDPFMENLYYHCEKNSMSIIIHIAPKIGGCYGLVDELGL
                     PRLEKILAKYPGLTILGHSHAFWSEISADNNEKIRNTYPTGKVIPGGRVVELMRKYPN
                     LHGDLSAGSGYNAVSRDEDFGCAFIEEFQDKLYFGTDICAPNNKPQLSFWLDRMMNEG
                     RISKEAYKKVSRENALWILAGAK"
     gene            complement(2824..4437)
                     /locus_tag="PK733_11055"
     CDS             complement(2824..4437)
                     /locus_tag="PK733_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11116.1"
                     /translation="MKKIISLLLAFVLVLSFTGCAKTSDKGNGNTESKTTDNQKQGSE
                     NEKTEPKFDTAGLALPISKDKVEIEYMISEHANNPVKEDWTVFQELEKVTNIKLKLQP
                     VGADYSQKLNLMVSSGQLPELFTSTVDFGKMYGVEGALIPISDYLKKMPNYSKFLAEN
                     PQTKKDLAASDGKIYNAVHYGHEVFGQGWLYRMDILKKHNLSVPKTYDEFYEVCKKLK
                     EIYPDSYPFSHRKKNGIAAEIGVQWGTGYGVYYNLDTKRFQYGCIEDNFRDMLMFLKR
                     LYDENLMDREWATISAKQWEDRMTNEQSFITYDYLARIKVFNPAMAEKNPEYDLEAAL
                     PPKAPNGKALIKTTYDKNGTAMEGFMVSKNNKHPEETFKLLDFLYSRYGQIITNLGVE
                     GATYKKDANGNLVWVDDIKSSISPNGTKHYTNDFGFIALGTYTVVTPEVRMLTREKGY
                     KEAVELYENNDVATGSDPQISMTEAELDVVKSKTSLGDYVDEQIMKFVLGERSLSEWD
                     AFVKEVKAKGVDELVKVYNDALDRYNKVN"
     gene            complement(4497..5393)
                     /locus_tag="PK733_11060"
     CDS             complement(4497..5393)
                     /locus_tag="PK733_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11117.1"
                     /translation="MKRKTSGLNNQTMSSKLFDIFNILLMLFICFVTLYPFLYVLATS
                     MSSNIAVMQNKVTFYPVELNFTAYKKILKDPYLVSSYYNTIKYTLIGTFINLLFTSTI
                     AYPLSRKNLVFKGFFNTMIIITMFFGGGLIPTFLLVNALGMYDTIWAIVIPGAISTYY
                     LIVMRTFFQSIPVELEESAKIDGASEVRVYFQIILPLSKAALAAIGLFYAVGHWNSFF
                     SALIYLKSKAKMPLQILLRNMLISSEMAQQNKIASRDEEVVVAETIKSAAIIVTVIPI
                     ICVYPFLQKYFVKGVMIGSIKG"
     gene            complement(5411..6382)
                     /locus_tag="PK733_11065"
     CDS             complement(5411..6382)
                     /locus_tag="PK733_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11118.1"
                     /translation="MNNAGISSSKIDLSRNRKKESYLFNKIKEIKRDKFLLLLILPGF
                     LYFVIFRYIPMGGILIAFKKYSVYKGILKSPWNHFQYFKEFFTDFNSWRLFRNTLLLG
                     VYSLFWSFPAPIIFALLLNEIKNLKFKKAVQTISYLPHFISTVIIVGMVLNFLSPETG
                     IINRLIMFFGREPIYFMIKNEWFRTVYISTGIWKGLGWSAIIYLAAISNINEELYDAA
                     KIDGAKRFRQALHVTLPGIAPTISVLFIMNVGGILGADFERVLLLQNPAIYETADIIS
                     TFVYRRGIAGADFGYATAVGLAQSIVGVVLLVSANYISKKFSENSLW"
     gene            complement(6615..8678)
                     /locus_tag="PK733_11070"
     CDS             complement(6615..8678)
                     /locus_tag="PK733_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11119.1"
                     /translation="MKKKMKIRVLSSIIVLLAFILPILIQPAFVHGAAEQDKAAITIN
                     PSYNTLTGKLSVQGKITGGNTEDGIKVTIKILNPSNKLLYLGEEISSADGSFVFEGMK
                     TNEEGTYKIYVGSEITEKAFMVNFKVQKETPVEPDLQPKPEEPGSEAGSSAGNLPPKP
                     EDKNKPTVGQIKEKFNEVLESIDITPADSTISALENIIGLVGDLSEHEQNDIKEELVS
                     MINKTIKKLSSLNIGMLAEKDGNVIITDEEASLNVEIKDEVISAAIKETNQYYKKITE
                     TLKLKGLTQAADTILSNRKVITILTDKSEKDLNISLMVKSIDALSSNNIDIVIDSTKA
                     GIESSSTQILSLRIPSGTLDMEYILKYLKDKNKDTNDIEGIKNSRVVIALKEMDKKDE
                     KEYIQNITDEQKSHRYAVYINVSPVIDLQFILDKEDEICIEKFNGNLFIEIPLDNEKI
                     SNNKVNTASKKIGVYCFNPDKKVWEYVGGRVNPDTGRMAFLTNHLSIYSVYEYNKTFK
                     DIQDNWAREFIEVLASRHIINGVNDEIFQPERNVTRTEFSKMLLEALDLVDYEAKTNF
                     TDVPVNAWYYKYVASAQKLGIAKGTTGNLFKPEEVISRQDMAVMAYRAVKAAGIGLPQ
                     EKEYKEFTDNRDISAYAKEAVEVLQQAGIFEGNGKGMFLPNNSSTRSEAAKIIYMLVE
                     ALELK"
     gene            complement(8786..11722)
                     /locus_tag="PK733_11075"
     CDS             complement(8786..11722)
                     /locus_tag="PK733_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11120.1"
                     /translation="MKCKSLLSIMTTLLSLILFTVVCYAIPLDNESNVSNSYGIASEN
                     SNAVNVTDYGAKDFVEPIECQLNGTNVIAVANGIGNLSVGDNIKIINGVGIRGTHKVK
                     LTLTGLPSKPIKVFFSMYYSSSGTYDLDPANHTLKDIMDMLVSDSRLNQYWTITRIDD
                     VTIEAESKAENDLTQKTATQLYGIAAKQPDETYDGTWSMEILRVGSPGVDLTAKITTI
                     EENNIILDKKCGSTTTTKAYLDSTQAFKDAFNFANSNNYDVVIPDGNYYIDSDIYIQT
                     NVRSTGKLYTSNKGTNSLFIISRKESPVKINGSSIAGSLEAGTRNIPELEQYADYDVI
                     FDSQERIMWRNNGVGQFYSKREANRINSDGSLLVDLAESYNDKSLLTMNLYKTESPIR
                     LEGLDILCIDNTITARGKNIIQVKRSDVTFDSLILDNENLSTKEGQNTGIGISDVVNI
                     VINDSKIRGFMLDGYGYPVLVSRTLYVMLNNTAISEARHAVAGNADNYTIINGGSYEG
                     YSGTLDSHWGHNYTVNNATVKGSIVYDGENFTINNCNITTESPTLIGRRMDCPTIKGN
                     VEIKDTTVTYTGTSDFNFYRAELTDFRHDAEIYNPNLIIENVKLVLENDIPAVDIYAI
                     NHHLNAVYIMQKLPETIRVKDLYITGKDDAKLDNTHYRMMAATNMYYTGNPNIYLENI
                     KINRNIQLDGSMNYDGEIAYSFLPNNTSIPDTHYNVEMKDCGTLSVNIVPTILGDMII
                     DNCIIVNYSYAGDLTNDWPNNPVWWAKGKGKTVFKNIKFDRTGLATSSISVMNDAEFE
                     NCEFINYGTIDYMDFGEQLPTVKILKVKDCIFNEGGITGGSLSEFMTPGTGGDEPGGD
                     EPGGDEPEDILFTVGDINFTDLDGAKITKLIGDTDIKASVAISSSTENVVPAAIILAL
                     YDENERIVSFSIVSIDIAGNTEATLATGFRLPQNIDGCKVKAFIWDSWENMKPLSNVV
                     TFPLSK"
     gene            complement(12154..>13135)
                     /locus_tag="PK733_11080"
     CDS             complement(12154..>13135)
                     /locus_tag="PK733_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11121.1"
                     /translation="DRYINSYFTEAVTFQVENNTIISIINVEDNMVAVMNKIRLIADK
                     LGTEKDYVYFTVIVSQIYNEISRLNQAYKKVGELEKYRTLSEKTQIIVEGDTKVNDTL
                     YFTKMQKEQIENLFLKNKYEECIEHIISILMYNAEQGVSSFSMFLLSTEIFNSCNDVL
                     IKLFNKIPSDFNIKKMYSMLKEYETIEEYKKIWKNFIKEYSGYIYANKGEDDEIIYSV
                     KQYIKENYTSDISVDIIAEKLKMSRSYLSRYFKEKTNECLSDYINRYRIQIASELLKT
                     SSLPINDVYLSVGINNNVTFNRLFKKYTGMTPNTYRRINNFKEEQGLNSL"
BASE COUNT         3859 a         2491 c         2011 g         4774 t
ORIGIN      
        1 tattaggttt agaacaaact gcaaaggcaa aatttaacct ttgtttttag ttgttataac
       61 atgttatgtc atgtattatt atagtccctt tatttgcagg tgtcaagagg tgacaacaaa
      121 catgacacat tttcttacta tgaaataaaa tatggcaata tacgattttc caagtataaa
      181 atttgaataa agcttacgga ttgttatgtt taaggcaatg aaaaattcgt aattcacgac
      241 aaggatgtcg tgaggcgtca cctgagccat ggacggcaaa tgtgacgggt agaatttttc
      301 tgtagactta aacgctacaa tccattgctg aagtgaatat tatacgtgga aatcgatttt
      361 tgccatattt ttcttcaaat atagaaaatg tgtcccaata aacgcacttg acataacatg
      421 tattattata tatcatttaa tcatcaaaat tttattagta ataatgaggt gagttttctg
      481 gaaaataacg atttatctcc ttcgaaacct ttatttctac aaataaagga ggctataaaa
      541 gaaaaaatac ttaatggcga atatcaatat ggaagtaaaa ttccttctga aaatgaatta
      601 tgtaaattgt ttaacgtaag cagaataact gttagaagcg caataagcga ggctatgaag
      661 gaaggtttgc tttttacagt tcaaggaaaa ggcacatttg tgaaggattt aaaagataca
      721 aaaatatcac aggcccttcc ctatatcaat tcctttgaaa gtacaattaa aaacctgggt
      781 ttatcagcag gtacagagat actcaaggtt gaaactatcc ctgctgatat cgaaatatcc
      841 aagatactgg atataaacat cggcaccaat gtacttatat tgagtctcct tgggaaggca
      901 gataatgaag gttatgttat ttataattca tatttttcag aaatcatcgg gaataagatc
      961 tatgaacttg ctaagaagga agtagaaaaa ggtgctgctt tctcaacttt agatttatat
     1021 aaaaagctga accagcctgt tcctgcattc atagaacaaa cctttgaagc tgttgcagct
     1081 gatgcatcaa cctcaaaggt tttgaactta aaaaaggatt ctccgctttt tctggtaagc
     1141 tcaataatat atacaggaaa cagtgcaccc cttgaataca ggaaagcttt ttacaggtcc
     1201 gataaataca agtttcatat aaagcgtgaa ttgagcttgt gaactaaagc acgaattaag
     1261 cttttgacca tttgtttggg aattcaccca actttatgcg agtgatattt ttggagtcat
     1321 tatgcataac ttttatcccc aagtgaagca ggcttgcttc actttccact catttagcac
     1381 ctgcaagtat ccacagtgca ttttcacggc ttactttttt ataagcctct ttggatatcc
     1441 tgccttcatt catcattcta tccagccaga atgataattg aggcttgtta ttaggggcac
     1501 atatatctgt cccaaaataa agtttatcct ggaattcctc aataaaagca catccaaagt
     1561 cttcatctct ggaaactgca ttataaccgc ttcctgctga aagatcccca tgtaaattcg
     1621 ggtattttct cattaattca acaactcttc cacctggtat tacttttcct gttggataag
     1681 tgtttcttat tttttcgtta ttatcagcac taatttcaga ccagaaagca tgagaatgac
     1741 ctaggattgt caaccccgga tattttgcaa gaatcttttc cagacgcggt aatcccagtt
     1801 cgtcaacaag tccgtagcat cctcctattt tgggagcgat atggattata atagacatgc
     1861 tgtttttttc acagtgataa tataggtttt ccataaaggg atcatccata tataggttat
     1921 aacttacctc acctacccct ttagcgcctc tttccttgta atagcttata aaatatgaga
     1981 ggtcataatt cggagtgttg tcacccatac gtggatctat attgcagaac cagtcaaact
     2041 taccaggata tttttctacc agatacattg tttcctcgtt tgactgtccc tgtagaccgc
     2101 attcaaggca aaagcttgtc attataaccc ctttttcaac tcccagttta tcatacattg
     2161 cgattaattc ttcaggtgtt gcataggttg tgccagcatc ctttctcggt atccctttaa
     2221 atgctcttgt gtgtacatga atatctattt ttttcatttt aagctccatt cctgctgcgt
     2281 gcgaaatgaa ttcgcccttg caggagcaaa actttttcag gaaaaagttt ttatgggctt
     2341 aaattcattt cattcacgcg tgcaatttat tttttaaaat tcttgtgcaa aaaatatgca
     2401 catgcgtatt agtttttcag aaatttatca atttcctgca aactaagtct ccattcctgc
     2461 tgcgtgcgaa atgaattcgc ccttgcagga gcaaaacttt ttcaggaaaa agtttttatg
     2521 ggcttaaatt catttcagtc gcgcgtgcaa tttatttttt aaaattcttg tgcaaaaaat
     2581 atgcacatgc gcattagttt ttcagaaatt tatcaatttc ttgcaaacta agtctccatt
     2641 tctgattata gtctatagct caaaagccct ggttgttgta aaaacagaat acagccaacc
     2701 agagccttca gagtcttaaa tacgctcatt tatgcttaat tacgtttatt tatgttattt
     2761 atttcaccta tattacctat gttgcctatg tcatttatca gttaacttta ctttcatcac
     2821 ttctcagtta actttattat acctgtcaag ggcatcgttg taaactttta caagctcgtc
     2881 tacgcctttg gctttaactt ctttcacaaa agcatcccat tcagagagac ttctttcacc
     2941 aagtacaaat ttcattatct gctcatctac atagtctcca agagaagtct tgctctttac
     3001 aacatccaat tctgcttcag tcatgctgat ctgaggatct gaaccggttg caacgtcatt
     3061 attctcataa agctcaactg cttctttata gcctttttct cttgtaagca tccttacttc
     3121 aggtgttaca acagtatatg tgcccagtgc tataaaaccg aagtcattag tatagtgttt
     3181 tgtaccgtta ggacttatgg aactcttgat atcatcaacc caaaccagat tcccgttagc
     3241 atccttctta tatgttgccc cttctactcc caggttggtg attatttgac catagcgact
     3301 gtagaggaaa tcaagcagtt taaaggtttc ttcgggatgt ttattgtttt tggaaaccat
     3361 aaacccttcc atagcagttc cgtttttatc gtatgttgtc ttaattaaag cttttccatt
     3421 aggtgctttt ggaggtaaag ccgcttcaag gtcatattca ggatttttct cagccattgc
     3481 agggttaaat acctttatac gtgcaaggta gtcatatgtt ataaaactct gttcgtttgt
     3541 cattctatct tcccactgtt ttgcactgat tgttgcccat tccctatcca ttaggttctc
     3601 atcatagagc ctctttagga acatgagcat atctctgaaa ttgtcttcaa tacatccata
     3661 ctggaatctt tttgtatcaa gattataata aacaccataa cccgttcccc attgaacacc
     3721 aatttctgcc gctataccat ttttctttct gtgtgagaaa ggatagctgt ccggataaat
     3781 ctctttgagt tttttacata cttcataaaa ctcgtcatat gtttttggaa cactgaggtt
     3841 gtgcttttta agaatatcca ttctgtaaag ccagccctga ccgaatactt cgtgtccata
     3901 atgaactgca ttgtaaattt tgccgtccga tgctgccagg tcttttttag tttgaggatt
     3961 ctctgccaaa aactttgaat aattcggcat ctttttcaaa taatcactaa ttggaattaa
     4021 tgcaccttca accccataca tttttccaaa atcaacagtg ctggtaaata gttcaggcag
     4081 ctggccggat gaaaccataa gattaagttt ctgtgaataa tcagcaccta caggttgaag
     4141 ttttaacttt atattcgtca ctttttctaa ttcctggaac actgtccagt cttcttttac
     4201 agggttgtta gcatgctcag aaatcatata ctctatctca actttatctt ttgagatagg
     4261 tagagctagc cctgctgtgt caaacttggg ttctgttttt tcattctcac taccttgctt
     4321 ttggttatcg gttgtttttg attctgtatt gccgttccct ttgtctgatg tctttgcaca
     4381 tccagtaaag cttaatacta gaacaaaggc taataagagt gatataattt ttttcattta
     4441 acttgcctcc ttttattttg attctgtaat atttttacag aaaattgttg acacatttag
     4501 ccttttatag accctatcat tacacccttc acaaaatact tttgcaggaa aggataaaca
     4561 caaattattg gtattacagt aactattata gctgcagatt taattgtttc agctacaaca
     4621 acttcctcat ccctgcttgc tattttattc tgctgagcca tttcagatga gatcaacatg
     4681 ttccttagta atatctgtaa tggcatctta gcttttgatt ttagatatat caaagcgctg
     4741 aagaaactat tccaatgccc aactgcatag aacaatccga ttgccgcaag agctgctttg
     4801 gacaatggaa gtattatttg aaagtaaact cttacttcac ttgccccatc aatttttgca
     4861 gattcttcaa gttcaacagg gattgattga aagaatgtac gcattactat aaggtagtat
     4921 gtggatattg caccaggtat aactatggcc catattgtat catacatccc aagagcgtta
     4981 actagtaaaa atgtgggaat aaggccgcca ccgaaaaaca ttgttattat aatcattgta
     5041 ttaaaaaaac ctttaaacac aagattcttg cgggaaagcg ggtatgctat cgttgatgta
     5101 aacaaaaggt ttatgaatgt tccaatcaag gtatatttta ttgtgttata gtaactacta
     5161 accaggtatg ggtcttttaa tattttcttg taagccgtaa agttcagttc cacagggtaa
     5221 aatgtaacct tgttttgcat aactgcaatg ttgctgctca ttgaagttgc cagaacatat
     5281 aaaaatggat aaagagtaac aaaacatatg aacagcatta acaatatatt aaatatgtcg
     5341 aacaatttgg atgacatagt ttgattattt aaaccggaag ttttgcgttt caatatcata
     5401 cacatccttt ctaccataag ctgttttctg agaatttttt cgatatatag tttgcagata
     5461 ccagtagaac cactcctaca atagattggg cgagtcctac agcagtcgca tacccaaaat
     5521 ctgctcctgc aattcctctt ctataaacga aggtgcttat tatatctgct gtttcatata
     5581 tggcaggatt ctgcagaagc aatactcttt caaagtctgc acctagaata ccgcctacat
     5641 tcattatgaa caagaccgaa atagttggtg ctataccagg cagtgtaacg tgaagagctt
     5701 gtctgaatct ttttgcaccg tctattttag ctgcatcata cagttcttca ttaatattac
     5761 ttatagccgc aagataaata atagcactcc aaccgagtcc cttccatatt cctgtactta
     5821 tgtaaacagt tctaaaccat tcatttttta tcatgaaata aataggttct ctcccaaaaa
     5881 acataattaa gcgattgatt atccctgttt caggagatag gaaattaagc accataccaa
     5941 caataataac tgttgatata aagtgtggca ggtaacttat tgtttgcaca gcctttttga
     6001 atttcagatt ttttatttca tttaatagca aagcaaatat tattggagcc gggaaactcc
     6061 agaataaact atatacccct aacagcaaag tatttctaaa aagccgccag ctgttgaaat
     6121 ccgtaaaaaa ttctttaaag tactgaaaat ggttccatgg acttttcagt atacctttat
     6181 atacgctata ttttttaaaa gctattaata tgccgcccat tggaatgtat ctaaatatta
     6241 caaaatataa aaaaccaggt agtataagta ataatagaaa tttgtcacgc tttatttctt
     6301 ttattttatt gaataaatag ctttcctttt ttctgtttct agataaatca atcttactac
     6361 tagatatacc agcattattc atgtcatgtt ttcctcctcg tgatgcatac tgtagttttg
     6421 attgtatatc tgcttttagg agatgtaaag gtacaattcg ttactggaat aacttctggt
     6481 agcaaaaaga tacagagtgt attaaaaagc acaaaatgta atgttaaaaa agcaaggaag
     6541 tcctagattt aattaggatc gctcctcgct ttttactttt acttattact tttgctattc
     6601 ttttattatt acttttattt taattcaagt gcttcaacta gcatataaat aatctttgca
     6661 gcttctgatc ttgtagagct gttattaggc aggaacatac cttttccgtt tccctcaaag
     6721 atacctgcct gctgcagtac ttcaaccgct tcctttgcat aagccgatat atctctgttg
     6781 tctgtaaact ctttgtattc tttttcctga ggtaaaccta ttcctgcagc tttgacagct
     6841 ctgtaagcca taactgccat atcctgcctg cttattactt cttcgggttt gaaaagattt
     6901 cctgttgtgc cttttgcaat gccaagtttc tgagctgatg ctacatattt atagtaccat
     6961 gcatttacag gtacatcagt aaagttagtt tttgcttcat agtcaacaag atccagtgcc
     7021 tccaagagca tcttggaaaa ctccgttctt gtaacattcc tttctggttg gaatatttca
     7081 tcatttacac cgtttattat atgtctggat gccaacactt ctatgaattc ccgtgcccaa
     7141 ttgtcctgaa tatctttaaa ggttttgtta tattcataaa ctgaatatat gcttagatgg
     7201 tttgtcagaa aggccatcct tcctgtatcc gggttaaccc tgcctcccac atattcccac
     7261 acttttttat cgggattaaa gcaatatacc ccaatcttct tacttgctgt atttaccttg
     7321 ttattactga ttttttcatt atcaagagga atttctataa aaagattgcc attaaatttt
     7381 tcaatgcata tttcatcttc tttatctaat ataaactgca aatcaataac cggagataca
     7441 ttaatatata cagcatatct atggcttttc tgctcatccg taatattttg aatatattcc
     7501 ttttcatcct ttttatccat ttctttcaaa gcaattacaa ctctgctgtt ctttatccct
     7561 tcaatatcat tcgtatcctt attcttatct tttagatatt ttaatatata ttccatatcc
     7621 aaggttcctg atggtattct taaagataat atttgagtcg aactgctttc tatgcctgct
     7681 ttcgtagaat caattacaat gtctatgtta tttgaagata atgcatcaat cgatttaacc
     7741 ataagactaa tattaagatc tttttcactt ttatctgtta aaattgtaat tacttttctg
     7801 ttagaaagta tagtatcagc tgcttgtgta agtcctttta atttaagtgt ttctgttatc
     7861 tttttataat attgattagt ttctttaata gctgcagata ttacttcatc tttaatttca
     7921 acatttaaag atgcttcttc atcggtaatt attacattac catctttttc agccaacata
     7981 ccgatattta aagaagacag tttctttatg gttttgttaa tcattgagac taactcttct
     8041 tttatatcgt tctgctcgtg ttcagacagg tcaccgacaa ggccaataat attttcaagt
     8101 gcacttattg tactgtcagc aggggttatg tcaattgatt caagaacttc attaaatttt
     8161 tcctttatct gtcccactgt aggcttattt ttgtcttcag gttttggtgg taaatttcca
     8221 gctgatgagc ctgcctctga accaggctct tccggctttg gctgcagatc tggctccaca
     8281 ggtgtttctt tttgaacttt aaagtttacc atgaaagctt tttcagttat ttcacttcct
     8341 acatatattt tatatgttcc ctcttcattt gttttcatgc cttcaaaaac aaatgagccg
     8401 tctgcggaac taatttcttc acctaaataa agtagtttat tagaaggatt taatattttt
     8461 atagtaacct ttataccatc ttcagtgttt cctcccgtta tttttccctg tacagaaagt
     8521 ttgcctgtta aggtattata ggaagggttt attgtgatag cagctttgtc ctgctctgca
     8581 gctccatgca caaaagctgg ctgaattaat ataggcagga tgaaggcaag gagtactatg
     8641 atgctgctta aaaccctgat tttcatcttt ttcttcatta cagtctactc ccttcttaac
     8701 atcctggggg gttgtttacc accccaggtt atagttttta tattttggcg ataatacttg
     8761 ctcaacatca aaatttaatt gtatattatt tgctcagtgg gaatgttacc acatttgaaa
     8821 gaggtttcat gttttcccaa ctatcccaaa taaatgcctt cactttacag ccatcaatat
     8881 tctgaggtaa tctaaaacct gttgcaagag ttgcttcagt atttccggct atatctattg
     8941 atactattga aaagctgaca atcctttcat tttcatcata cagtgctaag ataattgctg
     9001 caggtactac attctcagta ctgctgctaa ttgctacaga tgctttaatg tctgtatcac
     9061 caattagttt agtaatttta gctccatcta aatctgtaaa attgatatct cccaccgtaa
     9121 aaagaatatc ttccggttca tctccacctg gttcatctcc acctggttca tctccacctg
     9181 tcccaggagt cataaattca ctcaagctgc cgcctgtaat gccgccttcg ttaaaaatac
     9241 agtcctttac tttaagtatc ttcactgttg gtaactgctc cccgaaatcc atatagtcta
     9301 ttgtgccata attaataaat tcgcagttct caaattcagc atcgttcata acggatattg
     9361 aagaagtcgc aagaccagtt ctgtcaaact ttatattttt aaaaactgtt ttacctttcc
     9421 ccttagccca ccatacagga ttatttggcc aatcgttagt aaggtctcct gcataactgt
     9481 agtttactat tatgcaatta tctataatca tatcccctag tattgtaggt acaatattaa
     9541 cagatagtgt tccacaatct ttcatttcca cattgtaatg tgtatcaggt atacttgtat
     9601 tattaggtaa gaaagagtat gcaatttcac catcataatt catgctgcca tctaattgaa
     9661 tatttctgtt gattttgata ttttccaggt aaatattggg gtttcctgta taatacatgt
     9721 tggttgcagc catcattcta tagtgggtat tatctaattt agcgtcatct ttacctgtta
     9781 tatataaatc tttcactctt atagtttcgg gcagtttctg cattatatac acagcgttaa
     9841 ggtgatgatt tatagcatat atgtctactg caggtatatc attttccaat actaatttta
     9901 cgttttcaat tatcaggtta gggttatata tttcagcgtc atgtctgaag tctgttaatt
     9961 ccgccctata aaaattgaaa tcagaagtac ctgtatatgt tacagttgta tctttaattt
    10021 ctacattgcc ttttatagtg gggcaatcca tccttcgtcc tattaaggta ggactttctg
    10081 tagttatatt gcagttatta attgtgaagt tctcaccatc ataaacaatg cttcccttta
    10141 cagtagcatt gttcactgta taattatgtc cccaatggct gtcaagagtt ccgctgtaac
    10201 cttcatagct acctccgttg ataatcgtat aattgtctgc attacctgct actgcgtgac
    10261 gcgcttcact aattgcagta ttattaagca ttacataaag tgttctacta actaataccg
    10321 gatagccgta accatcaagc ataaaacctc tgattttgga atcgttaatc actatgttaa
    10381 caacatccga tatacctatt cccgtattct gaccttcttt tgtactaagg ttttcattat
    10441 ccagaataag gctatcaaat gttacatcgc ttctcttcac ctgaataatg ttcttacctc
    10501 ttgcggttat ggtattatca atacataata tatcaagtcc ttccaatcgt atgggacttt
    10561 ctgtcttata taggttcatt gttagtaatg atttatcatt atagctttct gccagatcaa
    10621 ccagaagaga accatcacta tttatcctgt tagcttccct cttggaatag aattgcccaa
    10681 caccattatt tctccacatt atacgttcct gtgaatcaaa tattacatca tagtctgcat
    10741 attgttccag ttcaggtata ttcctagtgc ctgcttcaag gcttccagca atactgctgc
    10801 cattaatttt tactggactt tctttacgcg agattataaa cagtgagtta gtccctttgt
    10861 ttgaagtata cagctttcct gtagatctaa cattagtttg aatatatata tcactatcta
    10921 tgtaataatt accatcaggt attactacat cataattgtt gctgtttgca aaattaaatg
    10981 catctttaaa agcttgagtg ctgtctagat atgcttttgt agttgttgtg ctgccacatt
    11041 ttttatcaag tataatattg ttttcttcaa tagttgtaat tttagctgtt aagtctacac
    11101 ctggactgcc aactcttaga atttccatac tccaggtgcc atcatatgtt tcatcaggct
    11161 gtttagctgc tattccatat agctgggtag ctgttttctg agtaagatca ttttccgcct
    11221 tactttcagc ttctatcgtt acatcatcga ttcttgtaat tgtccaatat tgatttaagc
    11281 gcgaatctga taccagcata tccattatat ctttaagagt atggttagca gggtcgagat
    11341 cgtatgtacc gctgctactg tagtacatag aaaagaatac cttaataggt ttagaaggaa
    11401 gaccggtaag cgtaagtttt actttatgcg tccctcgaat tccaactcca ttgataatct
    11461 taatattatc tcctacactt aaattaccaa tcccatttgc aacagcaatt acatttgtac
    11521 cattgagctg gcattctatg ggttctacaa aatccttcgc cccataatct gttacattta
    11581 ctgcatttga gttttcgctg gcaatgccat aactattact aacatttgat tcattatcca
    11641 atggaattgc ataacatact acagtaaaca gaatcaagct gagcagagtt gtcataattg
    11701 ataaaagact tttacatttc ataaaacaaa accctccttg atattttata tttggttaag
    11761 cataatcaaa aaactagtta gttaggtatt caaaaaatag attttttaat aaaaagaatg
    11821 gataataata tttaataata atttttttga aaatgcctaa tatgttaaaa attataattt
    11881 gtaggttgag gcaagtaaag aaacaatttg ttgtaaaagt aactggttgt tacacgaagt
    11941 aacaggatgt tttagaaagc acaaaatgtt attttagatt ttttcacttg ttttctattt
    12001 agttttacta tataatttac gatttgaaag agttaatgaa gaatttgcaa atcgtaagtt
    12061 acatcaaggt aacagaagca ggttttaatg tctaaaacct gttctatata gcaatttatg
    12121 cttgacccaa gtctatctca atttatatga ctttcataaa gagttcaatc cttgctcttc
    12181 tttaaaatta ttgattcttc tataggtatt tggagtcatt cctgtatatt ttttaaataa
    12241 cctgttaaat gtaacattgt tatttatacc tacacttaaa tagacgtcat ttattggcaa
    12301 ggatgaagtt tttaacaatt ccgaagctat ctgtattcta tacctgttta tatagtcact
    12361 caagcattca ttggtctttt ctttaaaata tcgggataag tagcttctcg acattttaag
    12421 tttttctgca ataatatcca cactgatatc ggaggtatag ttttctttta tatattgttt
    12481 aacactgtat attatttcat catcttctcc cttatttgca tatatgtagc ctgaatactc
    12541 tttaataaaa tttttccaga tctttttgta ttcttctatt gtttcatact ccttaagcat
    12601 ggaatacatc tttttaatat tgaaatctga tgggatttta ttaaacaatt ttattaatac
    12661 atcattacag ctgttaaaaa tctcggttga gagtaaaaac attgagaaac tgcttacacc
    12721 ctgttccgcg ttatacatta aaatgctaat aatatgctca atgcactcct catatttatt
    12781 ttttagaaat aaattttcga tctgctcttt ttgcattttc gtgaaataca atgtatcgtt
    12841 tactttagta tctccttcaa caattatctg agtcttttca gataatgttc tatacttttc
    12901 aagttcgccg acttttttat aagcctgatt taaccgtgat atttcattat atatttggct
    12961 gacaatcaca gtaaaataca cataatcttt ttcagtacca agtttatccg ctatcaatct
    13021 aattttattc attaccgcaa ccatgttatc ttcaacatta attatagaga taattgtatt
    13081 attttcgacc tggaatgtta cagcttctgt aaaataacta tttatgtacc tatct
//
LOCUS       DAOSDD010000116        13132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1837_length_13132_cov_4.920012, whole genome shotgun sequence.
ACCESSION   DAOSDD010000116 DAOSDD010000000
VERSION     DAOSDD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13132
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_13132_cov_4.920012"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1192
                     /locus_tag="PK733_11085"
     CDS             38..1192
                     /locus_tag="PK733_11085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11122.1"
                     /translation="MSLLDNRELNNFVDIKVCDTHFHLSFQHRIEITIGIFRDIRKYF
                     EYDRIALLALNETTSKGCDPTNSIKALYCKHCMSPYTYAFGSLFHYHDERDTSDSYLK
                     QARKMWEMGFDGVKMLEGKPGLRKKLGKPLDDPIFDAFYSYLEENSIPLTMHVADPIS
                     AWDISKVSDYALKVGWYYGDGTFPTKEQLHSEVDGILTKFPKLRLNLAHFYFMEEQLE
                     CADAFLDKWENVSFDLTANSQIYKYASLKPKKWHDFFVKHQNRLLFGTDTYNIPCKYE
                     ELEQNVGYRPNFLRTFLERYEPFEYPNFENKIIPLGMEYSVLENIYNNNCTRFLGAKP
                     REIDCNMAIEECQRLLHAFECGESKVTNESDVPIVLENLKIMIGDGNKKR"
     gene            1272..2576
                     /locus_tag="PK733_11090"
     CDS             1272..2576
                     /locus_tag="PK733_11090"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HOJ11123.1"
                     /translation="MEHSAIPTFSNVMKSLNISENVDILQKYWELSQTVFSIEDINFL
                     KKDYIIEVNKILQLPNEALKAFFDVQALIKNSPDLARYIWHCHYLLFHSIDYPDNNIL
                     KWPSLKACMGNQEGMFAAILCVSGIPAAVQYHKKKNIPYEVTIATFSDIRHRMKCYYD
                     RNGTWGLEPLRWFMEHIRGKIYKLSRLQFVLSSISDSEKVFRNISNKMVVAISEAGVK
                     YRSDGQIDGTNGVYDYESSWVSDLTIDSNYITGNVIRPDGYALKQAVRLDSTKWKQIL
                     NKGDTVLQIHIPVGGKMDFEECGHAFYEAMDFFPKFFPERPFIGFHCNSWLLDSQFEN
                     MLSPDSNIVKFLKEFYLLPFLSDDSYAMNVIFQGKPSNFKVAPRNTQLQRVVAEHYLS
                     GKHLRRMKGFILKDDLDWGKQVYRQNYNNFLYDYLFTALSYR"
     gene            2933..4213
                     /locus_tag="PK733_11095"
     CDS             2933..4213
                     /locus_tag="PK733_11095"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ11124.1"
                     /translation="MNEKLAIHGGPKAKTTPNIPMYPGGLEIGEEEKKQVLEVLDRKY
                     LFRYYGPEQYPSKVRELELKFAEKIGAKYALAVNSCTSALITALVACGVGPGDEVIVS
                     GYTFFASCAAIVAAKAIPVIAEVDETLTIDPEDIEKKITPSTKALVVVHMRGVPCNMD
                     KIMAIAEKHELRVIEDVAQAMGGTYKGKNLGTFGDCGCYSFQYHKIITAGEGGMVVTN
                     NQKLYDRCMGYHDTAACWRPDRFAEERYEGELFCGVNYRMSELTGAVMLAQLGKLYTL
                     LSQMKRNQSKILDQIKNTKRIKVRPVNDPDGDTGICIMFYLDSKDKIQPFIEALRAEG
                     IAAAGVFNSGIPDWHIYAHWKHVIEKKTPTLEGCPWTCPYHKGDPVEYSEEMNPKTLE
                     YLSRVIHIDIPSQLTLEDCDMIAKGINKVAAELA"
     gene            4347..5102
                     /locus_tag="PK733_11100"
     CDS             4347..5102
                     /locus_tag="PK733_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ11125.1"
                     /translation="MIKKSLCIESVLTDMDFYDRIKVAAEIGFDAIEFWDPVGKDIAK
                     IGRLAAENNISIAICCVKNAWITRMNFPSLIVEKNITESIKLAKEMGCCSLIALSGDV
                     EGKGDSQKNILIENLKRVAGIAVKENVTINIEALNSLVDHKGYYLDSSYIGFEIMKSV
                     DCDNIKLLYDIYHMQIMEGNIIDNIKRNIDFIGHFHSAGVPGRHEHFNGENDYKNILK
                     VVAETEYNRYFGLEYWPTYEHMQSLKDVLKYLS"
     gene            complement(5184..6218)
                     /locus_tag="PK733_11105"
     CDS             complement(5184..6218)
                     /locus_tag="PK733_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ11126.1"
                     /translation="MAKLTIKEIAKMAGVSPSAVSIVLNNRKGVSEETRNRVMEIVEK
                     LNYYPNPNSRRLLFNKTNNIGILFKKNASPMEHFFYSELNSIILQECEALGYNLMFSS
                     VAFNNKTNEVILPNVVNYRDVDGVIFYGDIDIQIINKFNTLDIPFIIVDSHHTSGDIL
                     SVSADYNEASYTATSYLISIGHTSIAFIGTNLIPFYGNQTFSGFKKAIEENRISLPIN
                     WIQLDAFDENSSYICMKNILSYNQLPTAVVCSADIFAIGAMRCLRDNGTKIPENMSII
                     GIDDILLAQYVDPPLTTVKIDKVKMGKIAIDLLVKKIENKKAENNVISSNEIMLRNST
                     RPANSLHSEI"
     gene            6466..8529
                     /locus_tag="PK733_11110"
     CDS             6466..8529
                     /locus_tag="PK733_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11127.1"
                     /translation="MDMMAQFESTEFRKTETDKTGTNKTMTDKAGTNITKINETENGM
                     TERVRIGIEVGEHGHNYESLTKDIAAKFDLKKLSFVLIYMSPVEIPERLWYEWAEFFR
                     ENDIHFAFLYTQQRGAPKGKISHLTVDIVEEIKRIAGEYFLGDMIGETGGLASWQEGY
                     YEDIGMDKPEFKNMSEAKQHYVDHVASRVKIDKDLKLPAVLAVEATTFSHYNFEAGVD
                     NTFIEMMCGNPEIMFASARGASKAHNRNWWASHIAQEWYGGFRNDDPLKYKRMKLAYY
                     YSFMAGAKYIYPESGHFKIESYGDNYGLESPFCEAYRKAWDEFADFLQQHERPVGGPK
                     VKIGFIQGNLDSWTGWGGATVWNQFRKEEWTYGASERGWDYLDEIFKSEKWHVPTVYG
                     ENDLTGSIPYGQYDIVPAEASIETLKQYSCLIMLGWNTMTPDIYNNLKQYVFQGGKLF
                     LSVPHLNTSDVRDDVRNGDFQLINNGDYSDLFGCKVRGKGMTVNWGVKFIEESSIPGY
                     LYPYTKSGYCDPICACGTVCCADIEVAGGRTIAIMADKFHKWRDASPVLLVENKYGKG
                     YASLITSWDYPGANGLEKFMKVLLKAIISGEQQSTDIKVIGSDKIHYAVYDNSGCGCN
                     TGTDCSMDNHTVDCSVISHNINDYVVYLLNTDYNVPAIAKIEYRGKTTEVKIEPCGFE
                     IAYIK"
     gene            complement(8591..9436)
                     /locus_tag="PK733_11115"
     CDS             complement(8591..9436)
                     /locus_tag="PK733_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11128.1"
                     /translation="MNSNIYSELLNLNMSPHAKISNPEGGAFHLHDTYEVYFFISGDV
                     TYFIEKSNYPLKYGDLIITNTTEIHTPVFHSDTTYERIIFHFSPEMINCLSFSEYSLE
                     RCFIDRPNGKLNKVSLNEQQIAEYFDIYRKIIFLNTDLNNGNIILKLSKLMELLVLID
                     KAFFNNEPNLAGQSSANIPDQLINIINYIDCNLMNNISLDSLGKIFYINKTYLCTLFK
                     KHIGKTVHQYIIYRRISYAKQLLASGYNVTETCLLCGFNDYSNFIKTFKNITGKPPGI
                     YKKGF"
     gene            9585..10640
                     /locus_tag="PK733_11120"
     CDS             9585..10640
                     /locus_tag="PK733_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11129.1"
                     /translation="MQKSKIKAAILGCGNISEAHARAIQVNSNKVELYAVCDIVEEKV
                     VKLVEKYSAVKYYTDYKEMLADDNIELVSICTPSGMHSEMAVDCAKAGKHVLCEKPLD
                     VTREKLDNMVQAFSNRELKFGTVFPYRTYPGLLTAKHILDSGELGRVYMGDGYCKVYR
                     SAEYYKSAGWRGTWELDGGGCLMNQGIHTLDVLCWLMGGVSSAEAKTFTIARDIEVED
                     TAFAFLKFRNGSYGVFQGTTLSYPGTGVKVEIQCEKGKIVFTDPNTYLYKTDDNGKEI
                     EICLDSIDDGNVEKGNTANDPSAIAITGHSWLMSDIVDAIIEDREPFVSVIEGRHAVD
                     VILAIYESSRTGKEIKV"
     gene            11014..12087
                     /gene="leuB"
                     /locus_tag="PK733_11125"
     CDS             11014..12087
                     /gene="leuB"
                     /locus_tag="PK733_11125"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOJ11130.1"
                     /translation="MSYNITLIKGDGIGPEIINAALPVLDKIGKIYNHNFQFEEILAG
                     GCSIDKIGEPITDEALEVCRRGDAVLLGAVGGPKWDNLSGNLRPEKALFRLRGELGLF
                     TNIRPAFLHHALRDACPLKKELTDKGIDLIIVRELTGGLYFGERGRYNKNGIETAFDT
                     MPYSVAEIERIGRVAFEIARKRRKKLCSVDKANVLETSRLWREVMHRLREDYPDVEYS
                     DMLVDNAAMQLVRAPYQFDVIVTENMFGDILSDEASIITGSIGMLPSASMGSTTFGMY
                     EPIHGSAPEIAGRDIANPIATILSVAMMLRYSFDLETEAQAIEKAVVEVLNKGYRTGD
                     IADKESVVVGTREMSRLVCESIN"
     gene            complement(12165..12878)
                     /gene="nagB"
                     /locus_tag="PK733_11130"
     CDS             complement(12165..12878)
                     /gene="nagB"
                     /locus_tag="PK733_11130"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOJ11131.1"
                     /translation="MKIIKATDYNDMSKKAAMIISAQVVLNPHSVLGLATGSTPLGTY
                     AKLVEWCNKGDLDFSDVITFNLDEYRGLTSEHEQGYRYYMNNNLFSKINIKPENTHVP
                     NGVANNIEEECSNYDKQISELGGISLQLLGIGHDGHIGFNEPAEYFEKSTHCVNLTEE
                     TIKANSRFFENADMVPKQAITMGMKSIMQAKKIVLVVNGKDKKEILNKALFGPITPKV
                     PASILQLHPDLTVIACDAL"
BASE COUNT         4511 a         1958 c         2705 g         3958 t
ORIGIN      
        1 cttaattttc gcctaactca caagaaagga gaaaagaatg agtctactcg acaatagaga
       61 actgaacaat tttgtcgaca taaaagtatg tgacacacat tttcatttaa gttttcaaca
      121 tcgaatagaa attacaatag gtatttttag ggatatcaga aaatatttcg aatatgacag
      181 aatcgcccta ttggctttga atgagactac atcaaaaggt tgcgatccta caaacagcat
      241 taaagcgtta tactgcaaac attgtatgtc accgtacaca tatgcttttg gaagcctttt
      301 tcactatcac gatgaacgtg atacatctga cagttatctc aaacaggcaa ggaaaatgtg
      361 ggaaatgggt ttcgatggag taaaaatgct ggaaggtaag ccgggactgc gtaaaaaact
      421 tggtaaacct cttgatgatc ctatttttga tgcattctat agctatcttg aagaaaattc
      481 aatcccgctg acaatgcatg tagctgatcc tatatcagcg tgggatatta gcaaagtttc
      541 tgattatgcg ttaaaagtag gatggtacta tggtgacgga acatttccaa caaaggaaca
      601 gcttcatagt gaagtagacg gaattttaac taaattccca aaattgaggc tgaatcttgc
      661 acacttctat tttatggagg aacagcttga gtgtgcagac gcatttttag ataaatggga
      721 aaatgtatca ttcgatttaa cagcaaatag ccaaatatat aaatacgcat cactaaagcc
      781 aaaaaagtgg catgatttct ttgtaaagca ccaaaatagg ctattatttg gaacagacac
      841 atataatata ccttgcaaat atgaagaact tgaacaaaat gtcggataca ggccaaattt
      901 tctgcgtaca ttcctagaaa ggtacgaacc ttttgagtat cctaactttg aaaataaaat
      961 tataccctta ggtatggaat acagtgtact ggagaatatc tataataata attgcacacg
     1021 ttttcttggt gcaaagcccc gcgaaataga ttgcaacatg gcgatagaag aatgtcagag
     1081 gttactccat gcttttgaat gtggcgaatc aaaggtcacg aatgaaagtg atgtaccgat
     1141 tgtattagaa aatcttaaaa tcatgattgg ggacggtaat aaaaaaagat aaacattgaa
     1201 aatcatacag taaaagcaac acattcgagc aaaatcaaag ttagattata gttagagagg
     1261 attgaattaa aatggaacac tctgcaattc caacatttag taacgtaatg aagtccctta
     1321 atataagtga aaatgtagat atccttcaga agtattggga gctttcacaa actgtatttt
     1381 ctattgaaga tatcaatttt ttaaaaaaag attatatcat agaagtcaac aaaattcttc
     1441 agctacctaa tgaggcctta aaagcatttt ttgacgtgca agcattaata aaaaacagtc
     1501 cagatttagc aagatacata tggcactgcc attatttact gttccactct attgattacc
     1561 cggataataa tatcctaaaa tggccgtcat tgaaagcctg catgggcaat caggagggga
     1621 tgtttgcagc cattctgtgt gtctcgggga tacctgcagc agttcaatac cataaaaaaa
     1681 agaatatacc atatgaagta acaatagcta cctttagtga cattagacat aggatgaagt
     1741 gctattatga tcggaatggc acatggggct tagagccatt aaggtggttc atggagcata
     1801 tccggggtaa aatttataaa ctatcccggc ttcagtttgt gttaagtagc attagcgata
     1861 gtgaaaaagt attcaggaat atttcaaata aaatggttgt ggccatctca gaagcaggtg
     1921 taaaatatcg gagtgatggg caaattgatg gtacaaacgg tgtatatgat tatgaaagtt
     1981 catgggtatc cgatttaact atagatagta actacattac tggaaatgtt atcaggccag
     2041 atgggtatgc tttgaaacag gctgtaagat tggacagcac aaagtggaag cagatactta
     2101 acaaaggtga taccgttctg caaatacata ttccggtggg aggtaaaatg gacttcgagg
     2161 aatgcggcca tgctttttat gaggcaatgg atttctttcc aaagtttttc ccggaaaggc
     2221 cttttatagg tttccattgt aattcctggc tcctggattc acaatttgaa aatatgcttt
     2281 caccagattc aaatattgtt aagttcctaa aagagttcta tcttttacct tttttgtcgg
     2341 atgacagcta tgcgatgaac gtcattttcc aaggcaaacc ctctaatttc aaagtagctc
     2401 ctaggaatac tcagttgcaa agagttgttg ctgagcatta cctgtcagga aaacaccttc
     2461 gcagaatgaa aggatttata ttgaaagatg atcttgattg gggaaaacaa gtataccggc
     2521 aaaattataa taattttctt tatgattatt tgttcacagc actatcatat aggtaaggca
     2581 agtaatacgg aatacttatg ataaaaaaga tttttaagat gcttattaac agcaaaaaaa
     2641 ataaaatgat aaaaaaataa tagagttaat gtatgaatat ggtaacaggg atgattaagg
     2701 ttaagaactt taggaaagaa aaagcaatat ttaagttcca taacttatca gctttgctaa
     2761 acacaaatat gtattgacaa tccaatttac taatgataat ctatattata taaatgcatt
     2821 tagtaaaagc cttatattta caccaaagaa ttataataaa tactcataag tacttgttat
     2881 taaaaacata actatatata tcaaaccatt tagtataagg aggtcatttc atatgaacga
     2941 aaagttagca atacatggag gaccaaaggc aaaaaccaca ccaaatattc ctatgtatcc
     3001 gggaggattg gaaattggtg aagaagaaaa gaagcaggtg ctggaggtct tagacaggaa
     3061 atatcttttc aggtattatg gtccggagca atatccttcc aaggtaagag aacttgaatt
     3121 gaaatttgca gaaaaaattg gagccaaata tgctctggcc gtcaactcat gtacatcagc
     3181 tttaataacc gcacttgttg catgcggggt aggtcctggc gatgaagtaa tagtatctgg
     3241 ttatacgttt tttgcatcat gtgcagcaat agttgcagca aaagctatcc ctgtaattgc
     3301 tgaggttgat gaaacattaa cgattgatcc tgaggacata gaaaagaaaa tcaccccttc
     3361 aaccaaagct ctggttgttg ttcatatgag aggcgtgccc tgcaatatgg ataaaattat
     3421 ggctattgca gaaaagcatg agctaagagt gattgaagat gttgctcaag caatgggggg
     3481 tacatataaa ggcaagaacc tgggaacatt tggtgattgc ggatgctatt ctttccagta
     3541 ccacaagatt attacagccg gagaaggcgg tatggtagtg acaaataatc agaaattata
     3601 cgacagatgt atggggtatc acgataccgc agcatgttgg agaccggaca ggtttgctga
     3661 agaaagatat gagggagagc ttttttgcgg ggttaactac agaatgagtg agttaacagg
     3721 agctgtaatg ctggctcaat taggaaaact ttatacgctt ctttcacaga tgaaaagaaa
     3781 tcaaagtaaa atacttgacc agattaaaaa tacaaagaga ataaaggtaa gacctgtaaa
     3841 tgatcctgat ggtgacacag gaatatgtat aatgttttat cttgatagca aagataaaat
     3901 ccagccattt attgaggcat tgagagctga aggaatagct gcagcaggag tatttaattc
     3961 gggaattcct gattggcata tctatgcaca ttggaagcat gtaatagaaa agaaaacacc
     4021 gacactggaa ggctgcccat ggacatgtcc ttaccataaa ggagatcctg tagaatattc
     4081 tgaagaaatg aaccctaaaa ctcttgagta tttaagtagg gtaattcata ttgacattcc
     4141 atctcaactt acattagaag attgtgacat gattgctaaa ggtataaaca aggtggcagc
     4201 agaacttgcg tgacaatctt gagttggtat tgtattaggt tgttttaacc atgagaaagt
     4261 tcaaatagaa aatgatgcag atacctaata aagcaaatgc ctaataatac ataaagataa
     4321 tgaataaaag ctgggagtga agcattatga taaagaaatc attgtgtatt gagtctgtat
     4381 tgactgatat ggatttttac gacagaatta aagtagctgc agaaataggc tttgatgcca
     4441 tagaattttg ggatcctgtg ggaaaagata ttgctaaaat cggacggctt gctgctgaaa
     4501 acaatatatc tattgctata tgctgcgtaa aaaatgcatg gataaccaga atgaattttc
     4561 catccttaat agtggaaaaa aatattacag aatctataaa gcttgcgaaa gaaatgggtt
     4621 gctgctcttt aatcgctttg tcgggtgatg ttgagggtaa gggagattct cagaaaaata
     4681 tacttattga aaacttaaag agagttgctg gtatagcagt aaaagaaaat gtaacaataa
     4741 atatagaggc tttaaattcc cttgtagatc ataaagggta ttatctggat tcatcataca
     4801 taggattcga aataatgaaa agtgtagact gtgataatat taaattgctt tatgatatat
     4861 atcatatgca aataatggaa ggaaatataa ttgacaatat aaaaaggaat attgatttca
     4921 taggtcattt ccattctgca ggagttcctg gaaggcatga acattttaat ggagaaaatg
     4981 attacaaaaa tattttaaag gttgttgcag aaacagaata caacagatac tttggtttgg
     5041 agtactggcc tacatatgaa catatgcaat cattgaagga tgttttgaaa tatctcagtt
     5101 aaggacctaa tacctgggtg aatgcgaact tatgtattcc ggaaagccat accaacgttt
     5161 agtgagggat tagcaatccc tcactaaatc tctgagtgga gtgaatttgc tggtcttgta
     5221 ctatttctta gcataatctc attagaactg atgacattat tttctgcttt tttattttct
     5281 atttttttta cgagcaggtc tatagctatt ttacccattt tcaccttgtc gatttttact
     5341 gttgtcaaag gaggatctac atactgagct aaaagtatgt catctattcc tattatggac
     5401 atgttttcgg gtattttggt gccattgtcc cgtaaacatc tcattgctcc tatagcaaaa
     5461 atatctgctg aacaaacaac ggcagtaggc agctggttat atgataaaat atttttcata
     5521 caaatatatg aagaattttc atcaaatgca tccagttgaa tccaatttat tggaagtgaa
     5581 attctatttt cttctatagc tttcttaaac cctgaaaatg tttgattacc gtagaaaggt
     5641 attaaattgg tgcctataaa agcaatggag gtatgtccaa tacttattaa atagcttgtt
     5701 gcggtatatg aagcttcatt ataatcggcg ctgacactta atatatcacc gcttgtatga
     5761 tgactgtcaa ctattataaa tggaatatcc agagtattaa acttgtttat tatctgaata
     5821 tcaatatctc catagaatat taccccatca acatcacggt aatttactac attcggcaga
     5881 ataacttcat ttgttttatt gttaaatgcg actgaagaaa acataaggtt gtacccaaga
     5941 gcttcacatt cttgcaaaat aatgctgttt aattctgaat agaaaaaatg ctccatagga
     6001 gaggcattct tcttaaataa tattcctata ttattagttt tgttaaaaag aagtctccgg
     6061 gaatttggat taggataata gttaagcttt tcaactattt ccatcactct attccgagtc
     6121 tcttcactta cacctttcct gttgtttaac acaattgaaa cggcagaagg tgatactccg
     6181 gccattttag caatttcttt tatagtcagc tttgccataa aacgttattg actccttgat
     6241 aaaaattctt gatttaattt aatgataaaa ggttttaatt tacctttaat acaattatac
     6301 tttatatttg tatataaaac aataaagcat tttatagagg gtgcattatc ttcaaaaata
     6361 aaacatgcac ccagaagctg atatgccgtt gttaacagaa atgccgtctg gtataataaa
     6421 catatagtaa gcagataatg tgaagtacaa gaaaggtgga aatgaatgga catgatggca
     6481 cagtttgaaa gtacagaatt cagaaaaaca gaaactgata aaacgggaac taataaaaca
     6541 atgactgata aagcaggaac taatataaca aaaattaatg aaacagaaaa cggaatgact
     6601 gaacgggtac gaattggcat tgaagttggt gaacatggtc ataattatga atctctaaca
     6661 aaagatatag cagctaagtt tgacctgaaa aagctgagct ttgtgcttat atacatgagt
     6721 cctgtagaaa tacccgaaag gttgtggtat gaatgggctg aatttttcag agaaaatgat
     6781 atacactttg catttcttta tacccagcaa agaggagctc ccaaaggtaa aatcagccat
     6841 cttacagttg atattgttga agaaataaaa aggattgcag gtgaatactt tcttggagac
     6901 atgataggag agaccggggg gttggcaagc tggcaggaag ggtattacga agatattggg
     6961 atggataaac cagaatttaa gaatatgagt gaggcaaaac aacactatgt agatcatgtg
     7021 gcatcaaggg ttaaaattga caaggatttg aagcttccag cagtgcttgc tgtagaggca
     7081 acaaccttca gtcattataa ttttgaagcg ggtgttgata atactttcat agaaatgatg
     7141 tgcgggaatc cggagattat gtttgcatct gcaagaggag cctcgaaggc acataaccgc
     7201 aactggtggg caagccacat tgctcaggaa tggtatggtg gatttagaaa tgatgaccca
     7261 ttaaaatata aacgtatgaa gcttgcctat tactattctt tcatggcagg agcaaaatac
     7321 atatatcctg aaagcggaca tttcaagatt gagtcctatg gggacaacta tggtttggaa
     7381 agtccattct gtgaggccta tcggaaagca tgggatgagt ttgcggattt tcttcagcag
     7441 cacgaaagac ctgtaggagg accaaaggtg aaaattgggt ttattcaagg caatcttgac
     7501 tcatggacag gctggggtgg agcaacagtc tggaaccagt ttagaaaaga agaatggaca
     7561 tatggtgcat ctgaacgggg atgggattac ctggatgaaa ttttcaaaag tgaaaaatgg
     7621 catgtaccta cagtttatgg agaaaatgat ttgacaggtt caatacccta tggtcaatat
     7681 gatatagtac cggcagaggc atcgattgaa acgttgaaac agtacagctg ccttattatg
     7741 ctcggctgga acacaatgac tcctgatata tataataatt tgaagcaata tgttttccag
     7801 ggcggaaaac tatttctgtc agtgcctcat ttgaacacat cagatgtgag ggatgacgtg
     7861 aggaatggag atttccaact tatcaataac ggagattatt cagatttatt tggctgcaaa
     7921 gtgagaggaa aaggcatgac tgtaaactgg ggtgtcaagt ttattgaaga aagtagtata
     7981 ccagggtatt tatatccata tacaaaaagc gggtattgcg atcctatttg cgcttgtgga
     8041 actgtatgct gtgctgatat agaagttgca ggaggcagga caattgctat tatggctgat
     8101 aaattccaca aatggcgtga tgcaagtcct gtattattag ttgaaaacaa atatggaaaa
     8161 ggttatgctt cattaataac atcttgggat tatcccggag caaatgggct tgaaaaattt
     8221 atgaaagtac ttttgaaagc tattatttca ggagagcagc agagtactga tataaaggtc
     8281 ataggaagcg acaaaatcca ttatgcggta tatgataata gtggctgcgg atgtaataca
     8341 ggtactgatt gcagcatgga caatcatact gttgattgca gtgttatcag ccacaatata
     8401 aatgactatg ttgtatacct tttgaatacc gattacaatg ttccggctat agcaaaaatc
     8461 gaatacagag gaaagacaac tgaggtaaaa attgaaccat gtgggtttga aatagcttat
     8521 atcaaataaa ttgaaaaggc ttttttgatt gttaatttgt tttcattata ttttagtcta
     8581 ccttatttta ttagaagcct tttttataaa ttcccggtgg cttgcctgtt atgtttttga
     8641 aggtttttat aaagttggaa taatcattaa acccacataa caggcaggtt tcagtaacat
     8701 tatagccgct tgccagaagt tgttttgcat atgatatcct tctgtatata atatattggt
     8761 gtacagtttt acctatatgc tttttaaaaa gagtacacag ataggttttg ttaatataaa
     8821 atatttttcc aaggctatca agagatatat tattcataag attacaatca atataattaa
     8881 taatatttat aagttgatca ggaatatttg cagatgactg accagccaaa ttcggttcat
     8941 tattgaaaaa agctttatca attaatacca gtaattccat cagcttgctt aattttaata
     9001 taatgttccc gttattcagg tctgtgttaa gaaaaataat ttttctgtat atatcaaaat
     9061 actcggctat ttgctgttca ttgagagaaa ccttatttaa cttaccattg ggcctgtcaa
     9121 taaaacacct ttcaagacta tattcagaaa aactcaagca attaatcatt tccggtgaaa
     9181 aatgaaatat tattctttca taagtagtat cagaatggaa aaccggtgta tgtatttctg
     9241 tagtattggt aataatcaga tctccatatt ttaaggggta gttacttttt tcgataaaat
     9301 aagttacatc acctgaaata aaaaagtaaa cttcatatgt atcatgaaga tgaaaagctc
     9361 caccttccgg gttacttatt tttgcgtgag gactcatatt taaattcagt aattcactat
     9421 aaatattgct gttcaacttg ggcgtgtacc tccttttggg ctaaaaacat ttttgcgtta
     9481 tcaaaagaaa aaccataaaa attgaaataa aagcaataaa cataaaataa ttatactata
     9541 gaatagtatt tgagtaaaac aaaaatggca gggagggtta ttttatgcag aaaagcaaga
     9601 ttaaagcagc aatactgggg tgtggcaata tttcagaagc acatgccaga gccatacaag
     9661 taaatagcaa caaagttgag ctttatgctg tttgcgatat tgttgaagaa aaggtagtaa
     9721 aacttgtaga gaaatatagt gctgtgaaat attacactga ttataaagaa atgcttgcag
     9781 atgacaatat agaacttgta tctatttgta caccaagcgg tatgcattct gagatggctg
     9841 tagattgcgc aaaagcaggc aagcatgtat tatgtgaaaa acctcttgat gttactcgag
     9901 agaagcttga taatatggtt caggctttca gcaatagaga attgaagttt gggactgttt
     9961 tcccttatag aacttatccg gggcttttaa cagcaaaaca tatattggat agcggggaac
    10021 ttggcagagt ttatatggga gatggttatt gcaaggttta tagaagcgcg gagtattata
    10081 agagtgcagg gtggaggggg acctgggaac ttgatggggg aggatgcctc atgaaccagg
    10141 gtatacatac tcttgatgtg ctatgctggc ttatgggcgg tgtatcaagt gcagaagcga
    10201 aaactttcac aatcgcaagg gatattgaag tagaagatac tgcttttgca tttttgaaat
    10261 tcagaaatgg cagttatgga gtatttcagg gaactacact ttcatatccc ggtacaggcg
    10321 ttaaagttga aattcaatgt gaaaaaggta aaatagtatt cacagaccct aatacttatt
    10381 tatacaaaac tgatgataat ggtaaagaaa ttgaaatttg tctggattct attgatgatg
    10441 gaaacgttga aaaaggcaat actgctaatg acccgagtgc catagcaatt acagggcata
    10501 gctggcttat gtcggatata gttgatgcta taattgaaga cagagaacca tttgtatccg
    10561 ttatagaagg aagacatgca gttgatgtta tcctggctat ttatgagtct tccaggacag
    10621 gaaaggaaat taaagtttaa ggttcgataa agagaattat ggcttaagat ttggtaaaga
    10681 gaactatagc ttaaggttcg gtaaagagaa ttaaggttta ggtttggtaa aggaaattaa
    10741 ggtttatagt tattgaattc aactctcctc agcatcatga atgaaagtaa taatcagtaa
    10801 aaaaaaactc ataaaagcta tgaatcagaa tagaaaatta aagggattta tagtaaaata
    10861 ataatataac gtcgtaatag aaggcttaaa cagttaaata ttatgttaat tagcgttagt
    10921 agacaaatta aactggacac taaagacata atataagatg cggtataatg caaaacctgg
    10981 tcaaatataa gctttaaaaa ggagagaggt atattgagct ataatattac attaattaaa
    11041 ggagatggaa taggtccgga aattattaat gcagccttac ctgtgttaga taaaatcggt
    11101 aaaatatata accataattt tcagtttgaa gagatattgg caggtggatg ctctattgat
    11161 aagatcggtg aacctataac tgatgaagcg ttagaagtat gcaggagagg tgatgcagtg
    11221 ctgcttggcg ccgtaggagg acctaagtgg gataatctgt cgggaaactt gcggccggaa
    11281 aaagctcttt tcagattacg gggagaactt ggattgttta caaatatacg tccggctttt
    11341 ctccaccatg cattacgtga tgcatgtccc ttaaagaagg agttaactga caaagggatc
    11401 gaccttatta ttgtcaggga attgacgggt ggcttatatt tcggtgagcg tggccggtat
    11461 aataaaaatg gaatagaaac agcttttgat actatgccat atagtgtggc tgaaatagaa
    11521 agaattggca gggtagcttt tgaaattgca cggaaacgca gaaaaaaatt gtgtagtgta
    11581 gacaaagcca atgttctgga aacttcaaga ctatggagag aagttatgca caggcttagg
    11641 gaagattacc ctgatgttga atattccgat atgctggttg ataatgctgc aatgcagctg
    11701 gtgcgtgctc catatcaatt tgatgtaatt gtaactgaaa acatgtttgg agatatttta
    11761 tctgatgaag caagtataat aaccggatct atcgggatgc tgccatctgc cagcatggga
    11821 tctactacct ttggaatgta tgaacccata catggttccg caccggaaat tgcaggacgt
    11881 gatattgcaa atcccatagc aacaatttta tctgttgcaa tgatgttaag atattccttt
    11941 gacttggaaa ctgaagccca ggcaattgaa aaagctgtgg tagaggtatt aaataaaggg
    12001 taccgtactg gtgatatagc cgataaggaa tcagtggttg ttggaacaag agaaatgagc
    12061 aggttggttt gcgaaagtat aaattgatta gtattgatat agatatggat aagctatgta
    12121 aggtgcaaaa aaggggagtg acaaatcact cccttaaggt tttattataa tgcatcgcat
    12181 gcgataacgg tcaaatccgg gtgaagctgc agtatggaag ctggaacttt cggagttata
    12241 ggaccaaaga gggctttgtt aagtatttcc ttcttatctt tcccattaac aacaagaacg
    12301 attttcttag cttgcataat tgatttcata cccatagtta ttgcttgctt tggaaccata
    12361 tcagcgtttt caaagaatct tgagttggct tttattgtct cttcagtgag atttacgcag
    12421 tgggttgatt tttcaaaata ttcggctggt tcattaaagc caatgtgacc atcatgacct
    12481 attccaagaa gttgaaggga tatgcctccg agttcgctga tttgcttatc ataatttgag
    12541 cattcctctt caatattatt tgctacaccg tttggaacat gtgtattttc cggcttaata
    12601 ttaattttgc tgaataaatt attgttcata tagtaacggt aaccttgctc gtgttcagaa
    12661 gtcaaaccac gatattcatc aagattaaat gtaataacat cggaaaaatc caaatcacct
    12721 ttattgcacc actcaaccaa ttttgcataa gtacccagtg gtgtagagcc tgttgcaagg
    12781 ccaagaacgc tatgtggatt cagtactact tgtgctgata taatcatggc agcttttttg
    12841 ctcatgtcgt tataatcagt agctttaata attttcataa ataacatcaa cctccatttt
    12901 acgctaaatt taacattata tgttattgta attacttaca tttataaaat cgatccattt
    12961 atgtataata gtttgattct tctcttttat actggatttc atacatatag tatattattt
    13021 attctgaaaa tgtcaaatat atctgggaga ggagggtgca ttttctatgc ttgagggaaa
    13081 atatggcaag aaacgatttc catgtataat attcacttca gcaacggatt gt
//
LOCUS       DAOSDD010000117        12986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1861_length_12986_cov_4.915242, whole genome shotgun sequence.
ACCESSION   DAOSDD010000117 DAOSDD010000000
VERSION     DAOSDD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_12986_cov_4.915242"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK733_11135"
     CDS             <1..380
                     /locus_tag="PK733_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ11132.1"
                     /translation="IAVGAYYLDGHKPDDDKRTYIIPREYLNVPPYDIPLRTLIAKDG
                     ENLMMAGRCMSADQLALSSARVTTTCSMTGQAAGITAALAVKSNKTVREVPAAKVQDI
                     IVNNGAILDKQKVKEVYLYRVKP"
     gene            675..3035
                     /locus_tag="PK733_11140"
     CDS             675..3035
                     /locus_tag="PK733_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11133.1"
                     /translation="MDKKKSRWSKFRKTYLKLLVSFLAFAILVFIVSFYIYGYSTNLL
                     RNEFKSKLETNFKHNISELDSSLTLNRSSLISIFYNPDHLQFFQPYEKMPLDVQVNIK
                     QIINVMSSAKTISSSYMDSVFYYLDDKKVYNRDGADDFITFFTKFHVYEGYNDDYWKE
                     KLVEYNLEETIGPVNLKIPVQIISAEKKIIAYQLTVIVNGNKIILVIDFDYDKIINAI
                     TINSLFLETGYIVLDNSNNVVYYTAPGNLKENRFENAVYNNDITHNINTIYNNKEYFI
                     CESNINLFGYKVLAVTPVRAFDKYLSNILIILVSSCFVFAITSLVLVMLFTNSIYRPV
                     NKISEFVAGNIGDKLAHKRDDTEFDMIRAGIEYLVEQSNSYKDNMLRVAEEFVKTSVI
                     NAINTGAPMNQMMFEILFGTQIKNEVISIKPEHSKKYFCCVTIIFRFAHSFLERSEEG
                     NVAYIFRSLKTIISSIFNEYMHCTVIEYEQGKFICVIGSFDEDINSKVKNAAKRMEES
                     FLFNSEYYNFFIGVGGTSSDICQIKQSYIEAATALVKAHTSSGERIVYIGEGKTSDSI
                     DLSREDELKIGQLLQSCELDPLMNIIEELTKRNMSNFLAHDKMLLFFTRLIEIGCSFM
                     ENMNYDCEDYLSIFADKIRGRIELVEPDYSDKIIFVMDFYGIVIEKHKKELNRSISNL
                     VENVKNYISKNYFKNISLESTAIQFNVSPKYLSRCFKEQQDINFTDFLNYARIKNAER
                     LLKETDMKIHEVAEAVGLPSKTTFVRLFKKYTSLVPRDYKRYLEVK"
     gene            3080..4006
                     /locus_tag="PK733_11145"
     CDS             3080..4006
                     /locus_tag="PK733_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ11134.1"
                     /translation="MNRKYMDIVDGVWPTMITPYTHDNKVDYKGLEELITWYKKKGVD
                     GLFAVCQSSEMFFLSLEERVEIAGFVKEKAGRDFPVIASGHVSDSIEDQIKEIKAISA
                     TGIDAFVVVSNRFAGQNESDDIWRINAEKVLCAVPDIPFGIYECPYPYKRLISPELLK
                     WCADTGRFFFLKDTCCNLEQLKAKVEAVRGSQLKIFNANSATLLDSMKFGISGFSGVM
                     ANFHPDIYVEITRQWKSNPEKTQILSDYAGFSSLAELQYYSMNAKYFLQLEGLNIGLH
                     SRSKSLEAFTGDKRLQIEQFFRFNKLIENKYL"
     gene            4324..4740
                     /locus_tag="PK733_11150"
     CDS             4324..4740
                     /locus_tag="PK733_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ11135.1"
                     /translation="MKSYRKELFFDIKKRREFVNITPQVQQCLRESGIKEGLLLCNAM
                     HITSSVFINDDESGLHRDFERFLEKLAPEKPYSQYDHNGYEDNADAHLKRTIMGREVV
                     VAITEGKLDFGPWEQIFYGEFDGNRRKRVLVKIIGE"
     gene            4862..6799
                     /locus_tag="PK733_11155"
     CDS             4862..6799
                     /locus_tag="PK733_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11136.1"
                     /translation="MTFTKKHYKAEAVKNSDKQKKISFIRKYIKNYWVPFLLAIVFLA
                     LEAFCDLMQPTIMSKIIDIGVAGKNVKYVISGGGLMLAVTALGAIAAIIRNIVSSNVS
                     QKFGCELRADLFKKIQTFSFDNIDKFEPASLITRLTNDVTQVQHFVHGTMRIFVKAPI
                     LCIGSFIMAWMLNPRMTVVLLVVVPIITFLIIMSAKIGYPFFAKVQKAIDDVNAVTRE
                     YLSGVRVVKAFNRFDYEKERFESSNRKLTSLTTTTMRIMTVFSPGMSLAVNLGIAAVL
                     WMGGIWVNYGQMNVGKIIAFINYMTQLLHSLMMISFVFNMFVRSRASTARIKEVFAQE
                     NTIPEPVNVYGVDDKYTGIADEDNANAGNASVDNANVDNVYPDIGDMKNEVIHENATT
                     SPKKAKVEFEDVSFLYAGASGEPVIKNVSFTCMPGETVGIIGSTGSGKTTLVNLIPRF
                     YDVTYGSVKIDGVDVRDIPTKKLRHMISIVPQKTILFTGTIIDNIKWGKDYATFGEIE
                     EAASAAQAHNFISAFPEGYDTLLGQGGVNLSGGQKQRVSIARALVKKPQILILDDCTS
                     AVDVATEAAIRKALKERSESMSCTVFIIAQRITSVMRADKILVLEDGALAGIGTHDEL
                     LKTCSVYHDIYHSQMGEDAVS"
     gene            7039..8862
                     /locus_tag="PK733_11160"
     CDS             7039..8862
                     /locus_tag="PK733_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11137.1"
                     /translation="MPGRPGGGFRGPRGMMPVEKAKDVKGTISKLWGLIRKEYGLLLI
                     VLMLIISASLISLRGPYLVGKSIDAMRLGKDAVDFDILKIMVTAFIVVYITDAVIVFI
                     QGWIMAGVSQRIVLKLRTALFAKLQKLPLKFFDSHPHGELMSRLTNDIDNVSSTISQS
                     TIQLMTSVITITGSLVMMIRLSPLLTLVSLITVPLVFILTRTIARKTKNFFKQQQVML
                     GKLNGHIEETISGFQVVKAFNYEEKSIQEFERLNTTLRESSIKAQIWSGFIMPLMNVI
                     NNLGFTAIAGFGGMLAVKDQITIGVIASFLSYSRQFTRPLNEIANTFNTLQSAIASAE
                     RVFEVLGEEEEPSDIPGALDINEPEGQVIFNDVSFGYRQDVPILKNISFRAEKGSRIA
                     FVGPTGAGKTTIVNLLVRFYDILEGEILVDGKDIREYKKESLMKYFSVVLQDTYLFSG
                     TIRENIRYGRLDATNEEVEDAAKMSNADAFIKRLPDGYETVLSESGGNLSQGQRQLLA
                     IARAFLARPTILILDEATSSVDTRTELHIQEALLKLMEGCTSFIIAHRLSTIRNADTI
                     IVINDGQILERGTHEELIEMGGFYSELYFSQFMGMETAQGS"
     gene            8945..12151
                     /locus_tag="PK733_11165"
     CDS             8945..12151
                     /locus_tag="PK733_11165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ11138.1"
                     /translation="MLLHGNFIHSEFKDNEPGIFIFWGENGNTLYKRRGKIPANAFPI
                     YPGLVRTDEIFQFLGQEKDEKETLVKNTSIGVLRHESYEIDGSNDSNGSSGSNNSNGF
                     NGSDDLNGSISSNGLIGSIIPYEIKGVSYSPWATLNILLTIEDKASKEAELDLAPEII
                     YWAQCAKFAAELIVKHRYYPKMGINQQGSLYSCWSMLADRNEDRERINILSKGMPPSC
                     KAVFLLSEWKNAADGKLEKYRFSGPNKILEVFINWIIDYIARSVIFDNNGIQLYTSLK
                     SDEERAWVRSIGDDKPDLVLEGGRKKQEPIYDIYKTWEASLKTSLIEQPFRTCFRITP
                     PEGREKVWKLDYFLQARDDQSLLVPAEEIWRKAGGAATFLQRNFGNPQERLLEDLSIA
                     SRAFEPILKSLYSAKPSGCTLSTEQAYNFFREGAVLLEESGFGIIAPSWWKKPVNISL
                     RLKVGIQGKSQGAVSKGVLNLNTLLEYDWKAAIGDVVLSEEDFIKLSKLKVPLVRVRG
                     EWVQLDRDQIEKLEEIWKRKGQEDAKRLGDILRMGLGNEELADGIPVESIVGDTELAD
                     FMQKLSSQDKLKEIPSPEGLHGNLRPYQKRGFSWLAFLRERGIGACLADDMGLGKTVQ
                     LISLALFERQEKMTDRPTLLICPTSVAGNWIKELEKFAPGMKVMLHHGHGRRSGSSFT
                     EEASRHDMVISTYSLAHRDEEEFKSVNWAGVVLDEAQNIKNPGSKQTMAVKRLKGDYR
                     IALTGTPVENRLSELWSIMDFLNPGYLGSNAYFRTHYAVPVEKDRNVEKSKKLKSIIA
                     PFVLRRLKTDPNIIKDLPEKIETKVYCGLTKEQATLYEAVVQDMLEKIEESDGIERKG
                     MVLAALSKLKQICNHPVQFLKDGSNIEGRSGKLERLFGILEEILAEGDKVLVFTQFAE
                     MGQILKNVVKKQFKTDAYFLHGGVPRKTREKMIESFQDDEKSPQIFILSLKAGGVGLN
                     LTRASHVVHFDRWWNPAVENQATDRAFRIGQKKNVQVHKFICSGTLEERIDKMLEEKK
                     ELAESIVETGEEWLTEMSAKELGELMRLNYNDVLVDED"
     gene            12245..>12986
                     /locus_tag="PK733_11170"
     CDS             12245..>12986
                     /locus_tag="PK733_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOJ11139.1"
                     /translation="MGRGKKDEFGSSWWAQKWNDALASYGWSNRLQRGRSYARTGHVL
                     EVNLKPGLVTAKVQGSRPRPYNVKIQIDKLTPSEWENVIGRMSKKAIFSAKLLTGEMP
                     ENIDEAFASAGTPLFPTSSESINTRCSCPDYANPCKHIAAVYYVIGQEFDRDPFMIFR
                     LRGMEKDELMEALRKARGGDVGVEDANEDNIDDSIGIEELQEEMQGFKGLDDAVFNMA
                     FSFTPPKVPYSVVKRLGTLPLFKEKEDFE"
BASE COUNT         4367 a         1948 c         2920 g         3751 t
ORIGIN      
        1 ctatagcagt tggagcatat tatcttgatg ggcataaacc tgatgatgat aaaaggactt
       61 atataatccc tcgcgaatat ctaaatgtac ctccttatga tataccatta aggacactta
      121 ttgcgaaaga tggagaaaat ctgatgatgg caggaaggtg tatgtcagca gatcaattgg
      181 cactttcttc tgcaagggtt actactactt gttctatgac cgggcaagcg gcagggatta
      241 cagctgcttt agctgttaaa agtaataaga ccgtacgtga agtacctgct gcaaaggtac
      301 aggatataat tgtaaacaat ggggctatat tagataaaca aaaagtgaag gaagtgtatt
      361 tgtacagggt aaagccctag gaatctgctt tttcgagcag actcataata aactgtggaa
      421 gcttattcgg agagcattat gaattctgat tcctgaattc tttggttgca gctgcatgcg
      481 tcagcatgtt ataataaata caatcaagga agtaaaacct agcgttttta caagatgtat
      541 ttattgaaac ggtgtgaagc aatccgattg gcggattgca cttatgttat aataaataca
      601 cagcatgatt tatgagttgc aggctggatg aaaatggtat agttatcatt atagaaatct
      661 gggggtatat aaatttggat aagaaaaagt ccaggtggag caaatttcgc aaaacatact
      721 taaaactact cgtaagtttt ctagcctttg caattcttgt gtttatcgta agcttttata
      781 tttatggata tagtacaaac cttttacgta atgagttcaa aagtaaactt gagactaatt
      841 ttaaacataa tatttctgaa ttggattcta gcctgacatt aaacaggtca tcgttgataa
      901 gtatatttta taatcctgat catttacaat tttttcaacc atatgaaaaa atgcctcttg
      961 atgttcaagt taacattaaa caaataatca atgtaatgtc gagtgccaag acaataagca
     1021 gcagttatat ggatagtgtt ttttactatc ttgatgataa aaaagtgtat aaccgtgatg
     1081 gagcagatga ttttataaca ttttttacca aattccatgt ttatgaaggc tataatgatg
     1141 attattggaa agaaaaattg gtagaatata atcttgaaga aactatcgga ccggtgaatc
     1201 taaaaatacc tgtacaaatt atatctgctg agaagaaaat aattgcatat cagcttactg
     1261 ttatagtaaa tggaaataag ataattttag tcattgattt tgattatgat aagattataa
     1321 atgctataac gattaatagt ctgtttttgg aaacagggta cattgtcctt gataatagta
     1381 ataatgtagt ttactataca gctcctggca atttaaagga gaacagattt gaaaatgctg
     1441 tatataataa tgatattact cacaacatca atactatata taataacaaa gagtacttta
     1501 tttgtgaaag caacattaat ttatttggct ataaggtttt agccgtaact cctgttaggg
     1561 cttttgataa gtacctatcc aatatactta ttattctggt ttcctcttgt tttgtatttg
     1621 ctattacgag tctagtactt gttatgttat tcactaattc catatatagg cctgtaaata
     1681 agataagtga atttgttgca ggcaacatag gggataaact tgcacacaag cgtgatgata
     1741 cagagtttga tatgataaga gcgggcatag aatatttagt tgaacaaagc aatagctaca
     1801 aggataatat gttgcgagtt gcagaggaat ttgtaaaaac atcagtaatc aacgctatta
     1861 acacaggagc accaatgaat caaatgatgt ttgaaattct ctttgggacc caaattaaaa
     1921 atgaagttat ttcaatcaaa ccggaacaca gcaaaaaata tttttgctgt gttaccatta
     1981 ttttcaggtt cgcacatagc tttcttgaaa ggtctgagga gggtaatgta gcctatatat
     2041 tcagatcctt gaaaacaata atttcttcaa tatttaatga gtatatgcat tgtaccgtta
     2101 ttgaatatga acagggtaag tttatatgcg ttattggctc atttgatgaa gatattaatt
     2161 caaaggtcaa aaatgcagca aaaaggatgg aggaatcctt cttatttaat tctgaatact
     2221 ataacttttt tattggggta gggggtacaa gttcagatat atgtcaaatt aagcaatcct
     2281 atattgaagc agctactgct cttgtaaaag cccatacttc atcaggtgaa agaatagttt
     2341 atattggaga gggcaaaact tctgattcaa tagatttgag cagggaggat gaattaaaaa
     2401 tcgggcagct tttacaatcg tgtgaattag atccattaat gaatataatt gaggaactta
     2461 caaagagaaa tatgtccaat tttcttgctc atgataagat gctgcttttc tttacccggc
     2521 ttatagaaat cggatgctca tttatggaga atatgaatta tgattgtgag gattatttaa
     2581 gcatatttgc cgacaaaata aggggcagga tagaattagt tgaaccagat tactctgata
     2641 agataatatt tgtgatggat ttttatggaa tagttattga aaaacataag aaggaactta
     2701 atagaagcat tagcaatttg gttgagaatg taaaaaatta tataagtaag aactatttta
     2761 aaaatattag ccttgaaagt acagctatac aatttaatgt ttcgcccaaa tatctatcaa
     2821 ggtgctttaa agagcagcaa gatattaact ttactgattt tctaaattat gcgcgcatta
     2881 aaaacgcaga aagactttta aaagagacag acatgaaaat acatgaagtg gcggaagctg
     2941 ttggtttgcc aagtaaaaca acttttgtaa ggttgttcaa aaaatacaca tcactggtac
     3001 caagagatta taaacgttat ttagaggtca agtgaacctc ttgatataga atacagacat
     3061 atttgaagga ggcaggagaa tgaacagaaa atacatggat attgttgacg gtgtatggcc
     3121 tacaatgatt acaccctaca cccatgacaa taaggtggat tataagggac ttgaagaatt
     3181 aataacatgg tataagaaaa aaggtgttga tggtcttttt gcggtttgtc aatctagtga
     3241 gatgtttttc ctctctcttg aggaaagggt ggaaattgct ggatttgtca aggaaaaggc
     3301 agggagggat tttcctgtta ttgcttcagg gcatgtatcc gacagtatag aagatcaaat
     3361 aaaggaaata aaggcaatat ctgcaacagg tattgatgct tttgttgtag taagcaaccg
     3421 tttcgcaggt caaaatgaaa gtgatgatat ttggagaata aatgcagaaa aggtgctttg
     3481 tgcagtgccg gacatccctt tcggtatcta tgaatgcccc tacccttata aaagattaat
     3541 ttcgccagag cttttaaaat ggtgtgcaga tacgggaaga ttcttcttcc ttaaagatac
     3601 ctgctgtaat ttagagcaat taaaagctaa agtggaagca gtaagaggaa gccaacttaa
     3661 aatatttaat gcaaattcag ctacattact ggattcaatg aaattcggca tatctggttt
     3721 tagtggtgta atggccaact tccaccctga tatttatgtt gagataaccc gccaatggaa
     3781 atccaaccct gaaaaaacac aaattttgag cgattatgca ggattttctt ctttagcaga
     3841 gttgcagtat tactcaatga atgcaaaata tttcctgcaa ttagaaggcc ttaatatagg
     3901 acttcattct agaagcaaaa gcctggaagc atttacaggg gataaacgtt tgcagataga
     3961 gcaatttttc aggttcaata aacttataga aaataaatac ctgtgaaggt aaatgtatga
     4021 gtttcaaaat aacaatcagc gaacatgttt ttgaaccaga aaaatatttt ccataactgt
     4081 gttatagcct gcatgggaga aatcctatgg aggcttttgt tatgcaggat taacgctaaa
     4141 tacagagtcc ttaaatctaa aatttaggat gtgtgcccaa gtactgggtg catattttat
     4201 ttttgaagaa attattggaa gaagcattag tggaggctct aagtactctt attaattgta
     4261 ataattatga aaatagttta taattaaaat cagttttaat tttgattttg caggaggtaa
     4321 ataatgaaaa gttacaggaa agaattattt tttgatatca aaaagagacg tgaatttgta
     4381 aatataacac cccaagtgca gcaatgtctg agggaaagcg gcatcaaaga aggtttactc
     4441 ttgtgtaatg caatgcatat tacctcaagc gtatttataa atgatgatga aagcggactt
     4501 cacagggatt ttgaaagatt ccttgaaaaa ttagcacctg aaaaacccta tagccaatac
     4561 gatcataatg gttatgaaga caatgctgat gcccatttaa aaagaactat aatgggacgg
     4621 gaagttgtag ttgcaataac tgaaggtaag ctggattttg gaccctggga gcagattttt
     4681 tacggtgaat ttgatgggaa tagaaggaaa agagttctgg ttaaaattat tggggagtaa
     4741 aaggaggtat cttgggagtg acgagggaac ttatccgaat gataagagga tgattaccta
     4801 ggagaaataa tgagctgtca ggtaataatg gcctattgta gaatgacaag gggagtgtgg
     4861 tatgaccttt acaaagaagc attacaaagc agaggctgta aagaattcag acaagcaaaa
     4921 gaaaataagc ttcataagaa aatatataaa aaactactgg gtaccatttt tattagccat
     4981 agttttttta gcactggaag cattttgtga tttaatgcaa cccactataa tgtctaaaat
     5041 aattgatatt ggtgtagcag gaaaaaatgt gaaatatgta atttccggtg gcgggctgat
     5101 gcttgctgtt actgcactgg gtgctattgc agcaattatt cgcaatattg tttccagcaa
     5161 tgtatctcaa aaattcggat gtgagttgag agcagatctt tttaagaaaa tacaaacttt
     5221 ctcttttgat aatatagaca agtttgaacc tgcttcactt attacaaggc ttacaaatga
     5281 tgtaactcag gttcagcact ttgtacatgg caccatgaga atattcgtaa aagctcctat
     5341 tctttgcatt gggagcttta ttatggcttg gatgcttaac ccgcgtatga ctgttgtttt
     5401 gctggttgta gtccctatca tcacttttct tataataatg agtgcaaaaa tcggataccc
     5461 attttttgca aaagtacaga aggcaatcga tgatgtaaat gccgttacaa gagagtattt
     5521 atccggtgta agggtggtaa aggcctttaa taggtttgat tatgaaaaag aacggtttga
     5581 gagttccaat cgtaaactta catcattaac aacgaccaca atgcgaataa tgacagtttt
     5641 cagccctggc atgtcgttgg cagtaaacct gggaatagct gctgtccttt ggatgggagg
     5701 catatgggtt aattatggtc aaatgaacgt tggtaaaatt attgctttta ttaactacat
     5761 gacgcagctg ctgcattcct taatgatgat atcatttgta tttaatatgt ttgtaagatc
     5821 aagggcttcc actgcacgta taaaagaagt attcgcacag gaaaacacca tacctgaacc
     5881 ggttaatgtt tatggagtag atgataaata tacgggcatt gccgatgaag ataatgcaaa
     5941 tgcaggtaat gcaagtgtag ataatgcaaa tgtagataat gtatatccgg atatcgggga
     6001 tatgaaaaat gaagtaatcc atgaaaatgc aactacatcc ccaaaaaaag caaaagtaga
     6061 atttgaggat gtgtcttttt tatatgccgg tgcatcaggg gagcctgtta ttaaaaatgt
     6121 atcttttacc tgcatgccag gagagactgt aggaattatt ggttctacag gttcagggaa
     6181 aacaactctg gtgaatctta ttcccagatt ttatgatgtt acatacggtt cggtgaaaat
     6241 tgatggtgta gatgtaaggg atattccaac taagaagtta cgccatatga tttccatagt
     6301 acctcaaaaa actatactat tcacgggaac aattattgac aacataaaat ggggaaaaga
     6361 ttatgcaact tttggggaga ttgaagaagc agcttcagca gcccaggcac ataattttat
     6421 ttcggcattt cccgagggat atgatactct attggggcag ggaggggtta atttatcggg
     6481 aggtcagaag cagcgtgttt ccattgcacg tgcgttggta aaaaaacctc aaatactaat
     6541 attggatgat tgtaccagcg ctgttgatgt agcgacagaa gctgctataa ggaaagcatt
     6601 gaaagagcgt tcagaaagta tgtcatgtac tgtatttatt atagcacaga gaatcacttc
     6661 agtaatgaga gccgacaaga tactcgtgct tgaagatggc gctcttgcag gtattggtac
     6721 tcatgatgaa ttattgaaaa cctgcagtgt atatcatgat atctatcact ctcagatggg
     6781 cgaagatgct gtaagttaag ggggctattt atgacacagt ttaataacag aagtaaggtt
     6841 aataattggg gaaattcaga tatagtacat tcaagaactc aacagtcggg aataatgcac
     6901 caggccggag ctcaaaattc aggaatgcaa caggtcaggt cacaaaaaac agaggctcag
     6961 cagtcaggca tgcaaagacc tgggacaggc ggaacgggag gcattcaagg aagaccggga
     7021 atgtccggaa tgggcgggat gcctggaaga cctggtggag gctttagagg accacgaggc
     7081 atgatgccgg ttgagaaagc taaagatgtc aaaggtacaa tttccaaact atgggggcta
     7141 atacgaaaag aatatggact acttctgatt gtgcttatgt taataatcag tgcctcatta
     7201 attagcttga gaggccctta tttggtaggt aaatccatag atgccatgcg gctggggaaa
     7261 gatgcagtgg attttgatat tttaaagata atggttacgg catttatagt agtatatata
     7321 actgatgcag ttatcgtgtt cattcaaggc tggattatgg cgggggtttc acagcgtata
     7381 gtcctcaagc ttcgtacagc gctttttgca aaacttcaga aactaccgtt aaagttcttc
     7441 gattcacatc ctcacggtga attgatgagc aggttaacaa atgatataga taatgtaagc
     7501 agtaccatat cgcaatcaac tatacaatta atgacaagtg tcataacaat aaccggttca
     7561 cttgtaatga tgattagatt gagtcccttg cttaccctgg taagtcttat tactgtgcct
     7621 cttgtgttta ttctaacccg tactattgcc agaaaaacaa aaaatttttt caaacaacag
     7681 caagtaatgc ttggaaagct gaatgggcac attgaagaaa caatttccgg ctttcaagtg
     7741 gttaaagctt ttaactatga agaaaagtca attcaggagt ttgaaaggct taacactaca
     7801 ctgcgtgagt ccagtataaa agctcagatt tggtcgggtt ttattatgcc tcttatgaat
     7861 gtaataaata atttgggttt taccgccata gcaggatttg ggggaatgct ggcagtaaag
     7921 gaccaaataa ctataggggt aatagcaagt ttcttaagtt attcaaggca atttacgcga
     7981 ccgcttaacg aaattgcaaa tacttttaat acccttcaat cagcaatagc aagtgcagag
     8041 agggtttttg aagtacttgg agaagaagaa gagccttcag atattcccgg ggctttagat
     8101 attaatgaac ctgaaggaca agttatattt aacgatgtat cttttggtta ccgccaggat
     8161 gtacccatac taaaaaatat tagctttaga gcagagaagg gaagtagaat tgcctttgtt
     8221 gggcctactg gtgcaggaaa aacaaccatt gtaaatctat tggtgcgttt ttatgatatc
     8281 ctggaagggg aaattcttgt tgacggtaag gacatccgtg aatataaaaa ggaaagcctt
     8341 atgaaatact ttagtgttgt gcttcaagat acatacctgt tttcgggaac aataagagaa
     8401 aatatcaggt atgggcgcct tgatgctacc aatgaagaag tggaggatgc tgcaaaaatg
     8461 tccaatgctg atgcttttat aaagcgtctt cctgatggat atgaaacagt attgtctgag
     8521 agcgggggaa acttaagtca aggacagagg cagcttctgg caatagccag ggcatttttg
     8581 gccagaccaa ctatcttgat tttggatgag gctacaagca gtgttgatac acgtactgaa
     8641 ttacatattc aggaagcttt gctaaaatta atggaaggat gcacaagttt tattattgcc
     8701 catagattaa gcactatccg taatgccgat actattattg taataaatga tgggcagatc
     8761 cttgagagag ggactcatga agaactgata gaaatggggg gcttctacag cgaactatat
     8821 ttcagccaat ttatggggat ggaaacagct caaggatcat aattgtatat tggataggtt
     8881 agaattttgt aaaagaaatc aggataggct aagataaaac aaaaaatggg aagggatatc
     8941 cataatgttg cttcatggaa attttataca tagtgaattt aaagataatg agcctggaat
     9001 ctttatattt tggggagaga atgggaatac cttatataag agacggggaa aaatcccggc
     9061 aaatgcattt ccaatatatc caggactggt caggacagat gaaattttcc aattccttgg
     9121 acaggaaaag gatgagaagg agaccttggt taagaatact tctattggag tgctgagaca
     9181 tgaatcatat gaaattgatg gttcgaacga ctcaaatggc tctagcggtt cgaataattc
     9241 caatggtttc aatggatcag atgacttgaa tggctctatc agttcgaatg gcttgattgg
     9301 ttctattatt ccttatgaaa taaaaggcgt ttcatactcc ccctgggcta cattaaatat
     9361 tttattaaca attgaagata aagcatcgaa ggaagcggaa ctggatttgg ctccggaaat
     9421 aatttactgg gctcagtgtg caaaatttgc tgctgaatta attgtaaaac acagatatta
     9481 tcctaaaatg ggaataaatc aacaaggttc actttattca tgctggagta tgcttgcaga
     9541 tcgaaatgaa gatagagaac gaataaatat tttgagcaaa gggatgccac cttcctgcaa
     9601 ggcagttttt cttttatcag aatggaagaa tgcagcagat gggaaattag agaaatatag
     9661 gttttcaggg cccaataaaa tccttgaagt atttataaat tggataattg attatattgc
     9721 cagaagtgtc atatttgata ataatggcat ccaattatat accagcttaa aatccgatga
     9781 agagagggcg tgggtaaggt ctataggaga tgataaacct gatcttgtgc ttgagggtgg
     9841 cagaaaaaag caggaaccta tatatgatat ctataaaaca tgggaggctt ctctcaaaac
     9901 atccttaata gaacaaccct ttagaacatg tttccgaata actcctcctg aaggaaggga
     9961 aaaagtctgg aaattggatt actttctaca ggcaagggat gatcagagtc ttttagtacc
    10021 tgccgaggaa atctggagga aagcaggagg tgcagctact tttttgcaaa gaaattttgg
    10081 caatcctcag gaaaggcttc ttgaggattt aagcattgcc tccagagctt ttgaaccgat
    10141 tcttaagagc ctttattcag ctaagccttc aggatgtacg ctttctacag aacaggcata
    10201 caattttttc agggagggtg cagtgcttct tgaggaaagt ggatttggaa taattgctcc
    10261 ttcatggtgg aaaaaacctg tcaatatatc actgaggctt aaagtcggga ttcagggcaa
    10321 atctcagggt gcagtatcaa aaggtgtgct gaacctcaat acgcttcttg aatatgactg
    10381 gaaagcagca ataggagatg tagtattaag tgaagaagat tttataaagc tttccaagct
    10441 taaagtacca cttgtaaggg tgaggggtga atgggttcag cttgaccgtg accagataga
    10501 aaaacttgaa gaaatttgga aacgaaaagg acaggaagat gctaaaagac ttggcgatat
    10561 tttgaggatg gggcttggta atgaagaatt agcagatgga attccggttg aaagtattgt
    10621 tggtgataca gaattggctg attttatgca aaaactttca agccaggaca agcttaaaga
    10681 aataccctca ccagaggggc tgcatggaaa cttaaggcca taccagaaaa gaggtttttc
    10741 ctggcttgca tttttaagag aaagaggaat aggagcatgc cttgctgatg atatgggcct
    10801 tggaaagaca gtgcagttga tttctcttgc tctttttgag cggcaggaaa aaatgacgga
    10861 caggcctact cttttaatat gtcctacttc tgtagcagga aactggataa aggagcttga
    10921 gaaatttgca cctggtatga aggttatgct gcaccatgga catggaagga gaagcggcag
    10981 cagttttact gaagaagcat cacgccatga tatggtaata agcacatatt cccttgcaca
    11041 tagggacgag gaggagttca agagcgtaaa ttgggcaggt gtagttttgg atgaggctca
    11101 gaacataaaa aatccgggct ccaagcagac aatggcggta aagaggttaa agggggacta
    11161 ccgtattgca ttaacaggga caccggtgga aaaccggctt tcggaactat ggtccataat
    11221 ggatttttta aaccccggat atctaggtag caatgcttat tttagaacgc attatgctgt
    11281 acctgttgaa aaagaccgta atgtggaaaa aagcaaaaag ctaaagtcta ttatcgctcc
    11341 gtttgttctg agaagattga aaacagatcc gaatattatc aaggatttgc ctgaaaagat
    11401 tgaaaccaaa gtatattgcg gattaacaaa agagcaggca acgctttatg aggctgtggt
    11461 gcaggatatg cttgagaaaa ttgaggaatc ggatggcatt gagagaaaag gaatggttct
    11521 tgcagcactt tcaaaactta agcaaatttg caatcatcca gtacagtttt taaaggatgg
    11581 aagcaatata gaaggaagat ccggtaaact tgagagactt ttcggcatac ttgaggaaat
    11641 acttgcagaa ggagataaag ttcttgtgtt tacccagttc gccgagatgg gtcaaatact
    11701 taagaatgtt gttaaaaaac agtttaagac agatgcgtat tttttacatg gaggtgtacc
    11761 cagaaaaaca agggagaaaa tgattgaatc tttccaggat gatgaaaaat cccctcagat
    11821 ttttatatta tctctaaaag cgggaggagt aggactgaat cttaccagag caagccatgt
    11881 tgtacacttt gaccggtggt ggaaccctgc tgtagaaaat caggccactg acagagcttt
    11941 ccgtattggg caaaaaaaga atgtgcaggt gcacaaattt atatgctccg gtactcttga
    12001 agagcgtatt gataaaatgc ttgaagaaaa gaaagaattg gcggaaagta ttgtagagac
    12061 aggtgaagag tggctgaccg aaatgagtgc aaaggaactg ggcgagttaa tgaggttgaa
    12121 ttataatgat gtgctggtgg atgaggacta aacagcagta actgaactac aatacaattc
    12181 tacgcaattc cggtggtgga ttacgtaatt ttggtagtgg caggtgcaaa ggaggagagt
    12241 aattatggga agaggtaaaa aagatgaatt cggaagcagc tggtgggcac agaaatggaa
    12301 tgatgcattg gccagttatg ggtggagcaa cagattgcaa agaggacgtt cctatgcaag
    12361 aaccggccat gtattagaag ttaatctgaa acccgggctg gtaacggcaa aagtacaggg
    12421 aagcagacca agaccatata atgtaaaaat acaaatagat aaacttacac cgtcagaatg
    12481 ggaaaatgta attggcagga tgtccaaaaa agctatattt tctgccaaac tactaaccgg
    12541 cgaaatgcct gaaaacatag atgaagcttt tgcttcagct gggacaccgc ttttccctac
    12601 atcttccgag agcattaata ccagatgttc atgcccggat tatgcaaatc cctgcaaaca
    12661 tatcgctgca gtgtattatg tgattggtca ggaatttgac agagatcctt ttatgatatt
    12721 ccgccttagg gggatggaga aggatgaatt gatggaagct ttaagaaaag ccaggggcgg
    12781 agatgtgggg gttgaggatg ccaatgaaga taatatagat gattccatag gtattgaaga
    12841 gctgcaggag gaaatgcagg gctttaaagg tcttgatgat gcagttttta acatggcttt
    12901 ttcatttact cctcccaaag taccctatag tgtagtaaaa cgcctgggga cacttcctct
    12961 ttttaaagaa aaagaggact ttgaaa
//
LOCUS       DAOSDD010000118        12978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1864_length_12978_cov_4.862880, whole genome shotgun sequence.
ACCESSION   DAOSDD010000118 DAOSDD010000000
VERSION     DAOSDD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_12978_cov_4.862880"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK733_11175"
     CDS             <1..785
                     /locus_tag="PK733_11175"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOJ11140.1"
                     /translation="GKDVELILGKANIGGCSLEKHWNLVEQCDLLPDVKPYNFYMTGA
                     ETCSATLKEALVSEKWDFVTLQQVSDLSWRAETFFPYIQKLYDLVRKLATQAQIVIHQ
                     TWAYRCDAEELKNYGITQEEMFAHLKQAYAEASKKLGCRIIPCGEAFQKARAIFNYTP
                     DESYNPENVQPLELPEQSKSLIKGYYWRTGNTITGKAELHIDGRHGNEKGCYLANAVW
                     YEMFTKNSILDNKFCPEGVSQEELDILKNVAHETMKEYGGSY"
     gene            969..1100
                     /locus_tag="PK733_11180"
     CDS             969..1100
                     /locus_tag="PK733_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11141.1"
                     /translation="MAMEKDNVIAKAEETLKKLGSINEIRAEYEYREKAIMKDPICK"
     gene            1272..2186
                     /locus_tag="PK733_11185"
     CDS             1272..2186
                     /locus_tag="PK733_11185"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HOJ11142.1"
                     /translation="MVGFIYGKNYKGLEYFNKKFPYQELEEAIRRKGEITPILLDALD
                     KLVEHPEIATENSDYMLHIYALHLLAQFKEKKAFPKIISSISLDPDDLDEILDDTITE
                     GLASILYSTFDGQLSLLQSTIENPKINIYARCAALNTYAELYSDKALSKETVISYLKK
                     LIYDEQYNDGSDLATHIQDVVIEKHLFEMIDDIQYLYDEDRIDIGINGKHDDFIDSIF
                     SYEYSQERVKYIDDTIAEMEWWACFEQTQKKHIQREMDVDEISNQMLKKEKGSPTLVE
                     IKNMKVGRNNPCPCGSGKKYKHCCLNKI"
     gene            2283..2870
                     /locus_tag="PK733_11190"
     CDS             2283..2870
                     /locus_tag="PK733_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11143.1"
                     /translation="MNELGKLSINKKRELEEDRKRWLKGYPKEYYSENDAENEENNSA
                     KCDARQEGQVLITDIYDRESIEIDKLVYLALHYRTIPLWVRRNMAAENENKIIYLTEA
                     CERFLTKCKEEGITSFEEYDEKYKIHYRSKEWFERFKKLLTGNSDDLENDGYNFWDNK
                     QDYNYEQEHNKVIKEEYNDIFEKVNKIMLDFSTNS"
     gene            2911..3357
                     /locus_tag="PK733_11195"
     CDS             2911..3357
                     /locus_tag="PK733_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOJ11144.1"
                     /translation="MKIFIDADGCPVVDIAVKIAKEYKLEIIIVKNYNHEILNDYATI
                     ITVDQTPDSADYYIGNNAVEGDIVVTQDYGLAALALSKGALCLNQNGLIISSENIDSL
                     LDQRYFNQEMRRKHKKYTKFKKRTIEQDKKFEISLIELIQKLIRDR"
     gene            4225..4476
                     /locus_tag="PK733_11200"
     CDS             4225..4476
                     /locus_tag="PK733_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOJ11145.1"
                     /translation="MIAISVTKARKNIYQILTDVNTNCQPITITNNRGKNGVLISEDD
                     WNAIQETLYLNSIPGVAESIVIGGNTPIEDCLSADEVEW"
     gene            4811..6982
                     /locus_tag="PK733_11205"
     CDS             4811..6982
                     /locus_tag="PK733_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11146.1"
                     /translation="MVTIKKVLSDILKNRKSIFWKFLISYILIVSITLITLVPFLLNL
                     YEKDFEFNLREYADLCDDILLEFVAEINLINNTIQSIHLTEGFKRLILIKGEIKLEDY
                     LYLELARKSMLTYSCLDNNIADVILMFNNNDIVISKYNAFDNSSSFFSYYNFDKLDVN
                     INKNKKSVKLYPEMVIKSDTYISKDVLPLSFTLYNGDSSYGFAYFFLDKHKLFNSLNY
                     IINNGGYIKIFYDNNPILTKGDTNNFDEYYNVNVTNNDLSLMLTLGIPRDLFYTNYAS
                     LLTVMVQYLVLAFLVGIILALILTIMRFKPIDELLSSIYESENIDIKNSNEFELVKDK
                     YKEIKNQNLVRQQQIEIYKKTVENNMIERVLSSQNIHTSVYEVPKVFCDKYIVCYSVI
                     EVREDLVYDNSTELYYNSVEYLKAKIDSNNIIHIITEDSFIVIISAVNENASNELATI
                     FSNYSCNGSVHITYSMSDIFYGLENINLAYEQARKRYYYFKKAQIDNILEDNNSNINI
                     NSLKNLYYLVISANKDEACQLVKNSFICDIAPMFDFEQLYYSIRGTLLNCLGQLSEIS
                     RKKINIPKYNISKRPSQIVDDLCKTIIKLCDLFLSERKSNNIELKDEILKYIQDNFAD
                     QNLYINTIAEHFSISEKYLCSFFKEHTGTTINTILNEMRLEYVAKLIATTDIPINTLY
                     LKAGFNTYNSLYKAFKRKYGMSLREYKTDFGAKSHSTVNTL"
     gene            7311..8234
                     /locus_tag="PK733_11210"
     CDS             7311..8234
                     /locus_tag="PK733_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11147.1"
                     /translation="MKKDQLVKDMYRYRQLYFLLALPIAYLIIFHYVPMLGTQIAFKN
                     FKPSEGIWRSKWIGLDHFSKFVNSYMFSRVIKNTIVVSLYSLIAGFPIPIILALALNC
                     LRCERWKKVIQTILYMPHFVSVVVIIGMLTQMFNPVVGLYGHIYRTLFEGMPPDLLSE
                     PKSFPHLYVWSGIWQNMGWNSILYFAALSHVDLSLHESAMIDGASRFKRIMHIDIPTI
                     LPTVVIVFILNTGHIMNVGFEKAFLMQNNLNLVASEVISTYVYKIGLTGLGGSNFSYA
                     TAIGLFNSVINLIILIIVNKIAKKVGEISLW"
     gene            8228..9112
                     /locus_tag="PK733_11215"
     CDS             8228..9112
                     /locus_tag="PK733_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11148.1"
                     /translation="MVKLSTKSDKMFMFIVNVTVVIITLIVLYPLVYVLSSSFSSPKA
                     VVGGKVVLFPVEPTLRGYQEVFSDKNIISGFFNSVFYTVSGTILSVSMTMIAAYSLSQ
                     KKLPFNNVLMFLFTFTMLFSGGMIPSYLLVSSLRLLNTPWAIILPGCISAYNLIITRT
                     FLQNIPEALREAANIDGCSDIKYFISIIIPLSSAIIAVLCLFYAVGLWSNYFSAFLYL
                     TNRRLFPLQVVLREILLKNQIAANSMLDSESLTHLSGMEELLKYSLIVVTSVPMLLLY
                     PFIQKHFLEGVMIGSLKG"
     gene            9134..11047
                     /locus_tag="PK733_11220"
     CDS             9134..11047
                     /locus_tag="PK733_11220"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOJ11149.1"
                     /translation="MFISKKAKNTITPVEMNIGNTIVFTLLNGFTNKIELISSNATIV
                     ETNKKDLTADYPAGGTLLRMDASILINGFPMNLRRFIGSQESFYEPYVINGMQLWFDA
                     AKCIFDIIQENHGECKPKKDVRFVLADATMPVCPEELSIWCELYGQNKIDIADCYDAD
                     DCYLGAYYGASAHGGLDINHPEGTPIYAPFRLDDQYYFNSLKNGDKNNRWRGVRHWND
                     ETTWVIQVHHTGIPLVDEYIPIENGTEIAKGAMVFVGSHNHSHFVFKIYEDGYEYIVD
                     PWILFHQMFINAKEDYLSISIKPACCISGEKIYFEAVGASRDAELFWRMSDGSYYYGR
                     SVKHVFTKPSIYSVLLYAKDKNGISCSLQYITVMGKEITSPRLSIEAEDRGFVQLQHP
                     YTMTFGEIIDMPNVLDIATFETEKRIFYKTIKLNNNSPLPFSVENCSIECSSDFISIE
                     SIDAENMVCKIKIDTTGLDNHEYLEMIEIKCNMLNGNQTIWIKVNILPEINSNTVICS
                     CTDKEFWKTGYTLVGHKFKNWYTSKGYSNIYLTNGYDLNKEIKAVFQPILKAGKYKVG
                     FCKHDKLDRLSIIKIKVKEKYGVKLIDYVPQGELEIGEFTFDAGNEGRVEILTGECIG
                     PVIIDAMYFKKID"
     gene            11204..12850
                     /locus_tag="PK733_11225"
     CDS             11204..12850
                     /locus_tag="PK733_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11150.1"
                     /translation="MFKKSILHMMIAVILLISIVLSGCSNQNPGGTVSKENPAKQTQE
                     LEEKGPYTLSITMPQDVLVEDYETNAFTKYIEENCNIDIKFNFLPADATEAKNKLTLI
                     LSSGEKLTDVINMNLSETEAFSYGQDGIFLDLTKYYETAVNIQKANSVYPQYNILKNS
                     MSPDGKTYFVPWLAKEEIGLAPQKAWICQKFLDELSMKAPTNAQELYDFAKAVKKNDP
                     NKNGSQDEIPIVGKKDTAAHWLMNAFIYYDTGNHYIVKDGVVDVAYRQEEYREGLKYM
                     HMLCKEGLFSPLSFTMDLNQLKQLADGDGQHNVVGVAFTFATSMLMNNYKENPFIKDY
                     VGLAPITGPNGVRTTSYAEPSVVPRWFVTKDCKNPELAFDVADFMWSEESYCFSRFGV
                     KGADWNPIDPAEKKQANIAGFVALINQPNSNWNKPQNSHWRTGCPTFSYDFLDSRLFD
                     GNNLNTTYTAGLISQEYNKVLPPKGTFISNLKFMTEELNEVNEIQATLKTYSEESLAR
                     FVTGDLNIESDWDNYIKELEKIGLKKFLEVSQTAWDRMNK"
BASE COUNT         4651 a         1695 c         2465 g         4167 t
ORIGIN      
        1 caggtaagga tgttgagctt atattaggaa aggctaatat aggaggatgt tctttggaaa
       61 aacactggaa tttggtagaa caatgcgatt tgcttccaga tgtaaagcca tacaactttt
      121 atatgacagg agcagaaact tgcagtgcca cactaaaaga ggctcttgta agtgaaaagt
      181 gggattttgt cactctacag caggtaagtg acttgagttg gagagcagag acattttttc
      241 catacataca aaaactatat gatttggtta gaaagcttgc aacacaggcg caaatagtaa
      301 ttcatcaaac ttgggcgtac cggtgtgatg ctgaagagtt gaaaaattat ggaattaccc
      361 aagaagaaat gtttgctcat ttgaaacagg cctatgctga agcctcaaag aaattgggat
      421 gcaggataat accatgtgga gaagcatttc aaaaggcgcg tgcaatattt aattatacac
      481 cggatgagag ctacaatcct gaaaatgtgc agccacttga attacctgag caaagcaaat
      541 ccttaattaa gggttattac tggagaacag gaaatactat tacgggaaag gctgaattac
      601 atattgatgg caggcatggc aatgaaaaag gatgttacct ggcaaatgct gtatggtatg
      661 aaatgttcac aaaaaacagt atacttgaca acaaattctg tccggaagga gtttcacaag
      721 aagaactgga tatcctgaaa aacgttgccc atgaaaccat gaaggaatat gggggtagtt
      781 attaattaaa ttttaattta agcataaaga gcctgttgta tttggtgccc ggcaccaaat
      841 acaacatgtt ttactgggca aaaatgtaca ctgagcaatt gaagcaaggt caattataca
      901 atgagcttgg tgaagcttta gaaagatggc ttatattttt agatgattcc cctgaggagg
      961 tgctgaaaat ggctatggaa aaagataatg tgattgccaa agcagaagaa accttaaaaa
     1021 aacttggaag cataaacgag atacgggcag aatacgaata cagagaaaag gcaattatga
     1081 aagatccaat ttgcaaataa gaacttttag aaaaaaatcg caacatatag aaaaaatcta
     1141 ataaatattt tataattagt atataaaaaa tgttaataat tgatatagta tagttaggta
     1201 tatttgctaa tgtatagttg cttaaataat agttgactgt aatatatcaa tttggtattt
     1261 tatggtactt aatggtggga tttatctatg gaaaaaatta taaaggctta gaatatttta
     1321 acaagaagtt cccttaccag gaattagagg aagcaataag aagaaaaggt gaaatcacac
     1381 ctatattact tgatgctttg gacaagcttg ttgaacatcc tgaaattgcc actgaaaatt
     1441 ctgattatat gctgcatata tatgcattgc atttattggc tcaatttaag gagaaaaagg
     1501 catttcctaa aattattagt tcaatatccc ttgatcctga tgacctggat gaaattttag
     1561 atgatactat tacagaagga cttgcatcaa ttttgtatag tacttttgat ggtcaattat
     1621 cgctactgca aagtacaatt gaaaatccaa agattaatat ctatgctcgt tgtgcagcct
     1681 tgaacactta tgcagaatta tattcagata aagcacttag caaagaaacg gtaataagct
     1741 atttgaaaaa attgatttat gatgaacaat ataatgatgg ctctgatttg gcaacgcata
     1801 tacaggatgt tgtcatagaa aagcatttat ttgaaatgat tgatgatata caatatttat
     1861 atgatgaaga tcgaattgat attggcatca atggtaaaca tgatgatttt atagatagta
     1921 ttttttccta tgagtacagc caagaaagag taaaatacat agatgatact attgctgaaa
     1981 tggaatggtg ggcttgtttt gaacaaacac aaaaaaagca tatacagagg gaaatggatg
     2041 tcgatgagat ttccaatcaa atgttgaaaa aagaaaaagg aagcccaaca ttggtagaaa
     2101 taaagaatat gaaggttggg cgtaataatc catgcccttg tggcagtggt aagaaatata
     2161 aacattgctg tctgaataag atttgagaaa agactgatga tggttttgat tcacctgatg
     2221 gttttgattc acctgaaacc ggtgaactga gactacatga aaataaagga ggaactattt
     2281 ttatgaacga gttaggaaaa ttatctataa ataaaaaaag agaattagaa gaagatagaa
     2341 aaagatggtt gaagggctat cctaaagagt attattcaga gaatgatgcg gagaatgaag
     2401 aaaataatag tgcaaagtgt gatgcaaggc aggaagggca agtattaatt actgatatat
     2461 atgacagaga atcgatagaa attgataaat tggtgtattt agcactgcat tatcgtacta
     2521 ttccgctatg ggttagaagg aatatggctg cagaaaatga aaataaaatc atatatttaa
     2581 cagaagcctg cgaacgcttt ttgaccaaat gcaaggagga aggaatcact tcgtttgaag
     2641 aatatgatga aaagtataaa attcactatc gcagtaagga atggtttgaa agatttaaaa
     2701 aactgttaac tggaaatagt gacgatctgg aaaatgacgg atataatttt tgggataata
     2761 agcaggatta taattatgag caagagcata ataaggtgat taaagaggaa tataatgata
     2821 tttttgaaaa ggtaaacaaa atcatgcttg atttttctac taatagttag gattttttaa
     2881 tattaacaac ctaatggggg tgggggttac atgaaaatat ttatagatgc tgatggttgt
     2941 cctgttgttg atatagctgt gaaaatagct aaagaatata agctggagat aatcatagtt
     3001 aaaaattata atcacgaaat tttgaatgac tatgcaacca tcataacagt ggatcaaact
     3061 ccggatagtg cagattatta tattgggaac aatgcggtag agggtgatat tgtagtcaca
     3121 caggattatg gacttgcagc tttagcatta tctaaagggg ctctctgttt aaatcaaaat
     3181 ggtttaatta tatcttcaga aaacattgat tcattacttg atcaaagata ttttaatcaa
     3241 gaaatgcggc gaaagcataa aaagtacact aaatttaaga agagaacaat agaacaggat
     3301 aaaaaatttg agataagcct tatagagctt attcaaaagt taatcagaga tagataattg
     3361 gctgaatgca gtgcctccca atatcaggag tgtcccaaaa atttgcagca agggtaatct
     3421 ttcattgagg aagattgcag aaaaaatcaa ggctgatacc gggtccatat agctcaaagc
     3481 agcgattgtc tgaccgggaa gttactgcat ggaagaaaag tacaaataac aagcaattcc
     3541 tgtatgtaat agtcctaatg ttatcagtaa aaatatttcc aatccgccgc taaaggaata
     3601 acagaaatag atacaaggct cttagatatt atccttgaac ttaatgaaaa aggatataaa
     3661 actatacttg gattattaca atcaaattga tgactataaa tgatgtaaat ctgtgcgtgc
     3721 gagcaggagc acatgttctt gttgttttca tgcaattttt gtataataag cattaagtca
     3781 tattgaaaat attattgtcg cacaaataac caggcagttt aggatataat aagaataaca
     3841 tatctcttga tgtacgccta tgcgtacgct ataataaaga tgaaagtcag ccgggcggtc
     3901 agccactaca atgacacggg tgttgtaaag gttgtgcctg gctaatatta caattagttt
     3961 tggtggtaac cggcatagta gcattttgca gtatacccat gatattttgg cggttgacgc
     4021 tttagcaggc tatcatatgg gacctaatca ctgtaaactg cacttaaaaa cttttttcgc
     4081 ctatgttgga ggtttgaaac ccaaacagtt tccagttaat ttgtggtaat agtgtaaggt
     4141 ttgtaactga aaaaactcat tttaaaacct tataaataat attatacgat ataattaaac
     4201 ttaataaaag aaggagatga tgtcatgatt gcgattagcg taacaaaagc cagaaagaat
     4261 atctatcaga tacttactga tgttaatacc aactgccagc caattactat tacaaataac
     4321 aggggtaaga atggtgtgct tatatctgaa gatgattgga atgctattca ggaaacatta
     4381 tatctcaact caattccggg ggtagcagaa tcaattgtta taggcggaaa tacccctatc
     4441 gaagattgct tatcggctga tgaggtggag tggtaaatgt atcgaatcgt ttatgatgag
     4501 caggctgcaa agaatattaa aaatcttaag tctgtaggat tggatggaaa agcaaaagaa
     4561 ttcatataac aacatctctc atgccatgat aagcctgctt tttggtccta ttcctatacc
     4621 ttttatatgt tctaactctg gaaattactc ttgtaattca ccacacttca tttctattat
     4681 tactcccctg gcatctgctt cattgccgaa cctctgaata gtaacctatc aaagaaagaa
     4741 taattatata tctgttgaaa atatattgaa taagaatata gaatatagta aaattcaggg
     4801 ggaggttatt atggttacta taaaaaaagt tttaagcgat attcttaaaa ataggaaaag
     4861 catattttgg aaatttttaa tttcttatat acttattgtt agcataactt taatcacatt
     4921 agtacctttt ttactcaatc tttatgaaaa agattttgaa tttaatctaa gggaatatgc
     4981 agatttatgt gatgatattt tattggaatt cgttgcagaa attaacttaa ttaataacac
     5041 tattcaaagt attcatttga cagagggctt taaacgtttg atattaataa aaggagaaat
     5101 aaagctagag gactatttgt atcttgagtt agcaagaaaa agtatgctta cttattcctg
     5161 cctggataat aatattgcag atgttatatt aatgtttaat aataatgata ttgtcatatc
     5221 caaatataat gcatttgata attcttcaag tttttttagc tattataatt ttgataagct
     5281 agacgtgaac attaataaaa acaaaaaaag tgttaaatta tatcctgaga tggtaataaa
     5341 atcagataca tacataagta aggatgtttt acctttatct ttcacattat ataatggtga
     5401 tagcagctat ggatttgctt actttttcct ggacaagcac aaattgttta actctttgaa
     5461 ttatatcatt aataatggtg gttatataaa aattttttat gataataatc ctattctaac
     5521 taaaggcgat acaaataatt ttgatgaata ttataatgtt aatgtaacaa ataatgattt
     5581 gtctttaatg ctgacattag gcataccaag ggatctgttt tatacaaatt atgccagctt
     5641 gttaactgta atggtacaat atttagtatt agcttttttg gtaggtataa tacttgcttt
     5701 aatcttaact ataatgagat ttaagccaat tgatgagttg ttaagtagta tatatgaatc
     5761 tgaaaatata gatataaaaa actctaacga atttgagtta gttaaagata aatataagga
     5821 aataaaaaat caaaacctgg ttaggcagca acaaatagaa atttataaaa aaactgttga
     5881 aaacaacatg attgaacgtg tattatcatc tcaaaatatt catacttcag tttatgaggt
     5941 tcctaaagtt ttttgtgata aatatattgt ttgttattca gttatagaag taagagagga
     6001 tttggtatat gataactcta ctgagttata ttataattca gtagaatatt taaaagccaa
     6061 aatagatagc aataatatta tacatattat cacagaagat tcctttattg ttataatatc
     6121 agcagttaat gaaaatgctt caaatgagtt agccactatt tttagcaatt acagttgtaa
     6181 tgggagtgta catataactt attctatgag tgatattttt tatggtttgg agaatattaa
     6241 tttagcatat gagcaagcca ggaaacgtta ttattatttc aaaaaggccc agatagataa
     6301 tatattagaa gataataatt caaatattaa tataaattca ttaaagaatt tatattatct
     6361 tgttatttct gcaaataaag atgaagcttg ccaactggta aaaaatagtt ttatctgtga
     6421 tatagcccct atgttcgatt ttgaacagct atattattca atcagaggga cattactaaa
     6481 ttgtttaggt caattatcag aaatatctag aaagaagata aatataccta aatataatat
     6541 ttcaaaaagg ccaagccaaa tagtagatga tctgtgtaaa acaattataa aattatgtga
     6601 tttgttttta agtgaaagaa aaagtaacaa tatagaatta aaagatgaaa ttctaaaata
     6661 tattcaagat aactttgcag atcagaattt atatattaat actatagcgg aacacttttc
     6721 tatatctgaa aaatatttgt gtagtttttt caaagagcat acaggaacaa ctataaatac
     6781 cattttaaat gaaatgcggt tggaatatgt cgctaaactt attgccacaa ctgatatacc
     6841 cattaatact ttatatctga aggctggatt taatacatat aatagcttat ataaggcttt
     6901 taaacgtaag tatggtatgt ctcttagaga atataaaact gacttcgggg caaaaagtca
     6961 ttcaacggtc aacacgttgt aatttgtaac acttcatctt gcatttttat aggtacgcac
     7021 tgattcagca ttgtgtttcc atgcttcatt caccttttgt accgaaattt gagtcaatgc
     7081 acatgtaact ttttatgcaa aaaatggttt tatatgttac taattttctt taatcttact
     7141 aatctcatag tttctttttt ttttaagacc tttattctta ctttggtaaa actaatttct
     7201 aaatttaata tttgcaattt gtgctatagg tgataatgtt ttattagcat tattacacat
     7261 tttaatttta taggaagagt gattagtgtg aaagactcat ggaggttttt atgaaaaagg
     7321 atcaattagt taaagacatg tataggtata ggcagttata ttttctatta gcattgccaa
     7381 ttgcttactt aataattttt cattacgttc ctatgctagg aactcaaata gcgtttaaaa
     7441 attttaagcc aagtgaagga atatggagaa gcaagtggat aggactggat catttttcta
     7501 agtttgtaaa ttcatatatg tttagtcgtg tcattaagaa tacgattgta gtatcattat
     7561 atagtttaat tgccggattc cctataccta ttattttagc attagcttta aattgccttc
     7621 gatgtgaaag atggaaaaaa gtaattcaga ctatattata tatgcctcat tttgtatctg
     7681 ttgttgtaat tataggtatg ttaacccaaa tgttcaatcc tgtagtagga ttatatggtc
     7741 atatatatag gacattattt gaagggatgc cgccagattt attatcagaa cctaagtcat
     7801 ttccacattt atatgtgtgg tcaggtattt ggcaaaatat ggggtggaac tctatactat
     7861 actttgcagc gctttctcat gttgacttaa gtcttcatga atctgccatg attgatggtg
     7921 cttcaagatt taaacgaata atgcatatag atattcccac tatattgcca actgttgtaa
     7981 ttgtttttat attaaatacc gggcatataa tgaatgttgg ctttgaaaag gcatttttga
     8041 tgcagaacaa tctaaatctg gttgctagtg aagtgatttc tacatatgtg tataaaattg
     8101 gcttaacggg attaggagga tccaatttct cgtacgctac agcgataggg ttatttaact
     8161 cagttattaa tttaataatt ttaataatag tcaataaaat tgccaaaaaa gtaggtgaaa
     8221 taagcttatg gtaaagcttt caactaaatc tgataaaatg tttatgttta tagttaatgt
     8281 tacggtagtt ataattacgt tgattgtatt atatcctcta gtatatgtgt tgtcatcatc
     8341 attttcttca cctaaagctg tggtgggtgg taaggtagtt ctatttccgg tagagcccac
     8401 cctcagagga tatcaggaag tattcagtga caaaaatata attagtgggt tttttaattc
     8461 agtattttat actgtttcag gtacgatatt aagtgtaagt atgacgatga tagcagcata
     8521 ttctttatct cagaagaaac taccatttaa taatgtttta atgtttcttt ttacatttac
     8581 tatgctattc agtggaggaa tgataccatc ttatttattg gtttcgtcat tacgtttact
     8641 taatactcca tgggcaatta tacttcctgg atgtataagc gcttataatt taattatcac
     8701 tagaacattt ctgcaaaaca taccggaggc gcttagagaa gcagctaata tagacggatg
     8761 ctcggacata aagtatttca taagtataat aatcccacta tcttctgcta taattgcagt
     8821 tttatgttta ttctatgcag ttgggctttg gagtaattac tttagtgctt tcctatattt
     8881 aacgaataga aggttgttcc ctttgcaagt tgtactaagg gaaatattac ttaaaaatca
     8941 gatcgcagcc aacagtatgc tggactctga aagcttaaca catctatctg gtatggagga
     9001 gttgctgaaa tactcattga tagttgtaac atcggttcct atgcttttat tatatccatt
     9061 cattcaaaaa cattttttag aaggcgttat gattggatcc cttaaaggat aagaaaaaaa
     9121 ggaggaaatg ttaatgttta taagcaaaaa ggctaaaaat actattactc cggttgaaat
     9181 gaatattggt aatactatag tttttacact attgaatggg tttactaata aaattgagct
     9241 tattagcagc aacgctacta ttgtagaaac aaataaaaaa gatttaactg ctgattatcc
     9301 tgctggtgga actttactta gaatggatgc cagcattttg ataaacggat ttcctatgaa
     9361 cttaaggaga tttataggat ctcaagagag tttttacgaa ccatatgtca ttaatggtat
     9421 gcaactttgg tttgatgcag ctaagtgtat atttgacatt attcaggaaa accatggtga
     9481 atgtaaacct aaaaaagatg taaggtttgt cttagctgat gctactatgc cggtttgccc
     9541 tgaagagtta tctatctggt gtgaattgta tgggcaaaat aaaatagata ttgccgattg
     9601 ctatgatgca gatgattgtt atttaggtgc ctattatggg gcttctgccc atggtggatt
     9661 ggatattaat catccagaag ggacacctat atatgcgcca tttaggttgg atgatcaata
     9721 ttactttaat tctcttaaaa atggagataa aaacaataga tggcgtggag ttcggcactg
     9781 gaatgatgaa actacttggg taatacaagt gcaccacaca ggaatccctt tagtggatga
     9841 gtatataccg attgaaaatg gaacagaaat agcgaagggt gctatggttt ttgttgggag
     9901 tcataatcat agtcattttg tattcaaaat ttatgaggat ggttatgagt atatagtaga
     9961 tccatggata cttttccatc agatgtttat taatgcaaaa gaggattatt tgagtatatc
    10021 tattaaacct gcatgttgca tttctggaga aaaaatatat tttgaagctg taggggcatc
    10081 tagagatgca gagctatttt ggaggatgag tgatggatct tactattatg gcaggagtgt
    10141 taaacatgta tttacaaaac caagcattta ttctgttcta ttatatgcga aagataaaaa
    10201 tggtatatca tgtagtttgc aatatataac tgttatggga aaagagataa catcgccaag
    10261 attatcaatt gaggcagaag atagaggatt tgtacagttg cagcatcctt atacgatgac
    10321 ttttggggaa ataattgata tgcccaatgt gcttgatatt gctacgtttg aaacagaaaa
    10381 gcgtattttc tataaaacaa ttaaattaaa taataatagt ccattaccat tctcggtaga
    10441 aaattgttct attgagtgta gtagtgactt tatctcaata gagtcaattg atgctgagaa
    10501 catggtgtgt aaaataaaga ttgatactac aggcttagat aatcatgaat acctcgaaat
    10561 gattgaaatt aagtgcaata tgcttaatgg gaatcaaaca atttggataa aggtaaatat
    10621 tttacctgaa ataaatagta atactgttat atgcagttgt actgataaag agttttggaa
    10681 aactggttat acgcttgtag gccataaatt taaaaattgg tatacctcta agggatatag
    10741 caacatttat ttaactaatg gatatgatct taataaagaa attaaagctg tattccagcc
    10801 aattttaaaa gcagggaaat ataaggttgg tttttgcaaa catgataaat tggatcgtct
    10861 ttctataatc aaaattaaag taaaagaaaa atatggagtt aagttaattg attatgttcc
    10921 ccaaggagag ctggagatag gagagtttac gtttgatgcg ggaaatgaag gcagagtcga
    10981 aatattaact ggcgaatgta taggacctgt aattattgac gcaatgtatt ttaaaaagat
    11041 tgattaacct aagcaggaag cgttttataa aagaggaagg caataaagga aggcaataaa
    11101 ggaaggcaat aatggcagtt gttgatgctg ctggttaaca tgtatttaaa gcacttagta
    11161 atagtgctgt aaataaaaaa ataagaaaat gggggattaa aagatgttta aaaaaagtat
    11221 tcttcacatg atgattgcag taattttatt gataagcatt gtgctgtcag gctgcagtaa
    11281 ccaaaaccct ggtggaacag tttcaaagga aaatccggca aaacaaactc aggaactaga
    11341 agaaaaagga ccatatactc tatctattac tatgcctcaa gacgttttag ttgaggatta
    11401 tgaaacgaat gcatttacaa aatatattga agagaattgt aatattgaca ttaagttcaa
    11461 ttttttaccg gcagacgcaa cggaagctaa gaataagttg acactgattc tttcatctgg
    11521 tgagaaacta actgatgtca ttaatatgaa tttgtctgaa acagaagcgt tctcatatgg
    11581 acaggatgga atctttttag acttaacaaa atattatgaa actgctgtta acattcaaaa
    11641 agcaaacagt gtttatccac aatataatat tttaaaaaac agcatgagtc cagatgggaa
    11701 aacctatttt gtgccttggt tagcaaaaga agaaattggg ttggcaccgc agaaagcctg
    11761 gatatgtcaa aagtttttgg atgaactgag tatgaaagca ccgacaaacg ctcaggaatt
    11821 gtatgatttc gctaaagccg taaaaaagaa tgatccaaat aaaaatggct cacaagatga
    11881 gataccgata gtaggtaaaa aagatacagc agcacactgg cttatgaatg catttatata
    11941 ttatgacact ggtaatcatt atatagtcaa agatggagta gtggatgtag catataggca
    12001 ggaagaatat agggaaggct taaaatatat gcatatgctt tgtaaagaag gcttattttc
    12061 tcctctgtcc tttacaatgg acttaaatca gttaaaacaa ttggcagatg gtgatggaca
    12121 acataatgta gttggtgttg cattcacttt tgcaacttca atgcttatga ataactataa
    12181 ggaaaatcct tttataaagg actatgttgg gttagcacct attactggac caaatggagt
    12241 gagaacaact tcatatgcag aaccatctgt agtacctcgc tggtttgtta ctaaggactg
    12301 taaaaatccg gaattagcat ttgatgtagc agactttatg tggtctgaag aatcatattg
    12361 ctttagtaga tttggagtaa aaggtgcaga ttggaacccc attgatcctg cagagaagaa
    12421 acaagcaaat attgctggtt ttgtagcttt gattaaccag ccaaattcaa attggaacaa
    12481 acctcaaaat tcacattgga gaacaggatg tccaacattt tcatatgatt tcctggattc
    12541 tcgtctattt gatggtaata atttaaatac aacttatacg gcgggtttaa tttcccaaga
    12601 atataacaag gtattaccgc ctaaaggaac atttatttct aatttgaaat tcatgacaga
    12661 agaacttaat gaagtaaatg aaattcaggc aacacttaaa acttattccg aagaatcgtt
    12721 ggccaggttt gtaaccggcg atcttaatat tgaaagtgac tgggataatt atattaaaga
    12781 acttgagaaa ataggattga agaaattcct ggaagtttct caaacagctt gggatagaat
    12841 gaataagtga gataagggta tccttataaa ttagcaaggg cgcatctttt acacttgacc
    12901 caaagattac cttgagtggt aatatgttct tttgaaatat attgacgtaa cccttagaat
    12961 gtagacaaag agaggatt
//
LOCUS       DAOSDD010000119        12954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1868_length_12954_cov_5.694938, whole genome shotgun sequence.
ACCESSION   DAOSDD010000119 DAOSDD010000000
VERSION     DAOSDD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_12954_cov_5.694938"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PK733_11230"
     CDS             complement(<1..1099)
                     /locus_tag="PK733_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11151.1"
                     /translation="MIYSFSRLYLGWSDYQKAKELKTIKDITSPFTDALKNFMFERGR
                     TNVVLSANEMISKENRSFIDERRVASDKFFEVGFSTMEGNYPEEAEVLRKEYEKFKAL
                     RIEVDEEMQKSIIQRDPDGRTVWYTNCTNYINLVSATLKKIGGRQLNNNMIANYNELI
                     IDTLRFRSIVGNESSIFTSAITGRKAINDDEYAALLFMRGESKQVWLDMENEIEMIGS
                     DNLIKAAQTVKDRYYRQFRTNQDKLLELVLKGQIYEGAGKEIANLSIPALDSILLLAD
                     EAIQEIDIENQKNMKNGYGSFIKGLLQLLAGILIIIFIPAYMRTRLIQPLDNIVELIE
                     NLSIGKTDINIPFLQRKDEIGKLANGVEMLQK"
     gene            complement(1276..2553)
                     /gene="serS"
                     /locus_tag="PK733_11235"
     CDS             complement(1276..2553)
                     /gene="serS"
                     /locus_tag="PK733_11235"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ11152.1"
                     /translation="MLDLKFVRENPEIVKQNIRNKFQDSKLGLVDEVIALDIELRKTK
                     QEAESLRADRNRISKQIGSLMGQGKKEEAQVLKNQVNADAKRLAELEIKENELDKKVR
                     NIMMTIPNIIDPSVPIGRDDSENVELERFGEPSVPDFEIPYHTEIMERLNGIDLDSAR
                     KVAGNGFYYLMGNIARLHSAVISYARDFMIDRGFIYCIPPFMIRSEVVTGVMSFAEMD
                     AMMYKIDGEDLYLIGTSEHSMIGKFIDTILPESALPQTLTSYSPCFRKEKGAHGLEER
                     GVYRIHQFEKQEMIVVCKPEDSMTWYHRMWKNTVDLFQSMDIPVRTLECCSGDLADLK
                     VKSVDVEAWSPRQKKYFEVGSCSNLGDAQARRLRIRVDGADGKYFAHTLNNTVVAPPR
                     MLIAFLENNLNSDGSVNIPVALQPYMGGMTVIK"
     gene            complement(3043..4071)
                     /locus_tag="PK733_11240"
     CDS             complement(3043..4071)
                     /locus_tag="PK733_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ11153.1"
                     /translation="MKRNMKTWIYNLLNSPDRKAMPILSFPGAEMKSINIIQVVNEAK
                     AQYECIKAITEKYPSIANVACMDLSVEAQAFGCEIKFVENEVPTVIKGAINDIEEVEE
                     LNVPEIGAGRTLEYIKAVELAAKNLKERPTLAGMIGPFSLAGRLFDMTEIMVNIMVEP
                     DGMKLLLEKTTQFLIEYAKAFKNAGANGIIIAEPAAGLLSPQHCHEFSSEYVRKIVEA
                     VQDEFFMAILHNCGNTENQVESMVSTGAMGLHFGNAVDMARIMPKIPWGRVAMGNVDP
                     AGVMKNGSIDEVLKKTGELLWETAIYKNFVLSTGCDVPPGTPQSNIQAFFEELESFNS
                     SVMAGETA"
     regulatory      complement(4207..4388)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4591..5586
                     /locus_tag="PK733_11245"
     CDS             4591..5586
                     /locus_tag="PK733_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ11154.1"
                     /translation="MARPNRKKSPEGLYHVMSRSISEINLYQTDEDKDHYLQLLKRYK
                     EEYHCSIYSYILMDTHVHIYINTCGADISKFMLCLNTAYVIYYNRKHQRHGHLFGGRF
                     ASFIVDNETYSLTLSAYIHNNAKDLPGYRGKEELYPYSSYGIYTGHRDNTEGIVDTDY
                     LLKHFSSNKKAARKSYRAFSESMCGTGIMKEIDDNIIRAYTENNYRSEKSYIVRNEIP
                     DELLAKIQEIFDKKISAGLLAPEGLTEELTLGLSVGLKAGLRLKYSRGTSNIRAFITY
                     TLRILCGYSYKQLCEYIGNMSLSGISKLSNKGFRLFKEHTLYQNAFNALIGAIWR"
     gene            complement(5826..5957)
                     /locus_tag="PK733_11250"
     CDS             complement(5826..5957)
                     /locus_tag="PK733_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11155.1"
                     /translation="MSYKGWDKLIEEFKASGKSQAQWCREKDLIDSLGHSFLHYEIS"
     gene            complement(6293..8416)
                     /locus_tag="PK733_11255"
     CDS             complement(6293..8416)
                     /locus_tag="PK733_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOJ11156.1"
                     /translation="MKKRLMSILLILCVALTLLPMTAFAAGGAATIKNDWYYLRCMDN
                     YLNITADGKAELRKLSKNEAFYVESKGGGAFTLKMKDGRYLGLEGTRKEGMRVIAVSK
                     PYTWNIYAESTNFDTEKTDIFSLRPPEVSTMLVNASGEKNADGTPIIIWTHKAKYGWY
                     NAPEHGEFRFITAYTSETPDTTGESWKTYKENGLIGYKDQNGKVVIPAQFEEARNFSQ
                     GIARVRAKDKDLFAYIDTTGKFITPSKYYGSICDHTVRDGLMRVGTWGDDITSALMNG
                     ERLDYWVDEKGKTIVVMKSGATFNYSMKAGFIDTTGKEVIPLQFDEANSFQNDMAAVY
                     KFQGKMGNVDTNKVGWIDTTGKLVIPYMSDGDLYYDGNVHNFKDGLVCYFVETDKDPF
                     TLPMAGIIDKTGKVIIPANKDEWFHSSNFGLLWRDGVIAVTPTKEVNAKGVPTKGGGH
                     SWSFSSLYDYSGKLIKKLEGYVYALPIVGGYTLAMHQLPEDVYSCYWTVFDRSGNIVI
                     DKAESNNFNLLNQGVGIENGYIFFGDSHYEISSMPAPAPAPIPPAAPAKQPSAPAGLI
                     AKPMDTKFMLNGAEVALPAYNISSNNYVKLRDVGALLKTRFDVRWEDGKVKLYNHAAY
                     SPVGGELVAIGTDSKSAIPSNTEFMLFDKWGQWVGNVADLTAYNIGGNNYTKLRDIAK
                     LFDFDVDWRDGKVWIEPDVSPYTED"
     gene            complement(9159..9281)
                     /locus_tag="PK733_11260"
     CDS             complement(9159..9281)
                     /locus_tag="PK733_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11157.1"
                     /translation="MTYTAFTPYAPIENPADLTEAAWNEWAEYFENSAKIEPRL"
     gene            complement(9360..10442)
                     /gene="rsgA"
                     /locus_tag="PK733_11265"
     CDS             complement(9360..10442)
                     /gene="rsgA"
                     /locus_tag="PK733_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ11158.1"
                     /translation="MDIKNYGKLNLADYGFIPTMMPEDVSGTPARITAVHKERYEIIC
                     EYGQVFGRLKTSIYFGECEESFPTVGDFVLIQYNCSGDSQIIKTLERKSKFARNDFSG
                     HAVGYVKTVKEQVVASNFDYVFIMASLNHDFNLKRIERYLTLAWQSGAIPVVVLTKAD
                     LTDEFTEQARAVEKIAADVGVYAVSAKTGYGIDRLSEYLKPHKTIVFLGSSGVGKSSL
                     VNALAGEEIMMVNEIREEDSRGRHTTTHRQLIMLSSGVMIIDTPGMRELGMWEVSTGL
                     GEAFTDVESYFGKCRFSDCQHKSEPGCAIKEAMANGELSMERWSSYLQIKREAKFADD
                     KAGFLLDKSKRNKSIAQLSRQNKNKL"
     gene            complement(10442..10594)
                     /locus_tag="PK733_11270"
     CDS             complement(10442..10594)
                     /locus_tag="PK733_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11159.1"
                     /translation="MTKMKGTFEYIKEIRGGWAINPRTRVQENEMKNKKKRRQKDKKL
                     IKDGFE"
     gene            complement(11460..11978)
                     /locus_tag="PK733_11275"
     CDS             complement(11460..11978)
                     /locus_tag="PK733_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11160.1"
                     /translation="MFEAKKDLEKGLDTIWSAKRVDDNYQNWIVRDLNLLQRHFEEDR
                     LSEDEYAYLAERGYIRTYGQYNGEHFAASQVVWIRSAETKQKILDVGDKIKGMHKAEF
                     DSLKAPFVKAVMDSTPKQLRKMQAYGMQYIFYSDGWFILHCLKELVNNGKLKLPTEEQ
                     KKSLSTIVVFNN"
     gene            complement(12811..12939)
                     /locus_tag="PK733_11280"
     CDS             complement(12811..12939)
                     /locus_tag="PK733_11280"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOJ11161.1"
                     /translation="MPVSKEQGHGFGTKSMAYIVEKHGGVFQFSVKDGWFIFQATT"
BASE COUNT         3677 a         2829 c         2185 g         4263 t
ORIGIN      
        1 ttttctgcag catttcaaca ccattcgcca gctttcctat ttcgtctttt ctttgtagaa
       61 atggaatatt aatatctgtc tttccaatac taagattttc tattaattct actatgttat
      121 ccagcggttg gatcaatctt gttctcatat aagcgggtat aaaaataatg atcagaatac
      181 ctgcaagtaa ttgcaataag cctttgataa aacttccata tccatttttc atattttttt
      241 gattttctat atcgatctcc tgtattgctt catcagccag aagaagtata gagtcaagag
      301 ctggtatgga taaattggca atttctttac cggcaccttc atatatttga ccttttagaa
      361 caagctcaag aagtttgtcc tgattcgtcc ggaattgcct gtaatatcta tctttaacgg
      421 tttgcgccgc ttttatgaga ttatctgaac caatcatttc aatttcattt tccatgtcca
      481 gccaaacctg ttttgactct cccctcataa ataatagcgc agcatactca tcatcattta
      541 tcgctttacg accagtaata gcagacgtaa aaatggaaga ttcgttacca acgatgcttc
      601 tgaaacgaag ggtatcaatg atcaactcat tataattggc aatcatattg ttattcagtt
      661 gacgtccgcc aattttctta agcgttgcac tgacaagatt aatgtagttt gtacaatttg
      721 tataccatac tgtccgacca tcaggatccc tttgtattat tgatttttgc atttcttcat
      781 ctacctcaat ccgtaaagcc ttaaattttt catattcctt acggagcact tccgcttctt
      841 ccggatagtt gccttccata gtagaaaatc ccacttcaaa aaatttgtca gaggccaccc
      901 tccgttcatc gataaaggaa cggttttctt tagaaatcat ctcatttgca gaaagaacta
      961 cattcgttcg acctctttca aacataaaat ttttcaaagc atccgtaaaa ggacttgtta
     1021 tatccttaat ggttttcaat tcttttgcct tctggtaatc agaccatcca agataaagcc
     1081 tggaaaatga ataaatcata acaatgccac ataatacagt aactaatatt aaaattttat
     1141 acaaaatcga gctgcaagta tatattcttt ttggatgaaa acctttcata gattacactc
     1201 cttgctttag tacacaattt tgctcaccga agaaaggttg gtgagtaata tgttcagctt
     1261 gttattcggt tttcattatt taatcactgt catgccaccc atatatggtt gtaatgcaac
     1321 tggaatattt actgaaccat ccgaatttaa attgttttct aaaaatgcaa tcagcattct
     1381 tggtggtgct actacagtat tgtttaacgt atgtgcaaaa tatttaccat cggctccgtc
     1441 tacacgaatc cttaaccggc gtgcctgggc gtcccccagg tttgaacagc ttccaacctc
     1501 aaaatatttt ttctgtctag gagaccaggc ttcaacatca actgatttta cctttagatc
     1561 tgccaggtca ccagagcagc actccaaagt cctgactgga atatccatgg actgaaatag
     1621 atccactgtg tttttccaca tcctgtgata ccatgtcata ctatcttccg gcttacagac
     1681 aacaatcatc tcctgtttct caaactgatg aatcctgtat acccctcttt cttccaggcc
     1741 atgtgctccc ttttcctttc taaagcatgg tgaataactg gtaagtgttt gcggtagagc
     1801 tgattccggc agaatagtat caataaattt accgatcatg gagtgttcgc ttgtaccaat
     1861 caggtataaa tcttcaccat caattttata catcattgca tccatttctg caaagctcat
     1921 aacaccggtt acaacttcac tgcggatcat aaaaggcgga atacaatata taaaaccgcg
     1981 gtctatcata aagtctctgg cataagaaat gactgctgag tgaagtcttg cgatattccc
     2041 catcaaataa tagaaaccat tacccgccac tttcctggca ctatccaggt caataccgtt
     2101 caacctttcc ataatttcag tatgatacgg aatttcaaaa tcaggtacag aaggctcgcc
     2161 aaaacgttct aactctacat tttcgctgtc gtctctgcct ataggaacac ttgggtcaat
     2221 aatattgggt atagtcatca taatatttct aacttttttg tccagttcat tttcttttat
     2281 ttctaactca gctaaacgtt tagcatctgc attaacctga ttttttaata cttgcgcttc
     2341 ttccttttta ccttgaccca ttaaagaacc aatttgcttt gaaattctat ttctatcggc
     2401 tcttaaggat tctgcctcct gctttgtctt tctcagttca atatcgaggg ctattacctc
     2461 atccaccaaa ccaagtttac tgtcttgaaa tttgttcctt atattttgct tgacaatttc
     2521 agggttttcc cttacgaatt ttaaatccaa catgtttttt cctccccgta taaatattga
     2581 taatgctatc tttaaattaa aaataaaatc ctttcacccc gcatatatgg gacgaaagga
     2641 ttccgcgttg ccacccaaat tgccggtatc aagcataacc ggcctctcaa ctacagtatg
     2701 ataaaggata ccagcctttc agttcgtcac tgacagctac aaaagtggaa agatttaacc
     2761 tctcaccgat tcacaccaac catcggctct cttcagaaat ttataaatca tagcttttat
     2821 aatcgccatt ttttaatatg ttatttgttt tattatatat acaatttaag caaatttcaa
     2881 tacaatatat attgttatta ttatatattg ttattatgct caaatctttg tatttacttg
     2941 caggatcggt tccaggtgaa ttttgcaaca gcgcattatg ctaatattgg tataaaaatt
     3001 tgaaaccctt caaacatata atttatagta gccaatacct ttttacgctg tttctccagc
     3061 cattacagag ctattgaagc tttcaagctc ttcaaagaag gcttggatat tgctttgtgg
     3121 agtcccgggc ggtacatcgc aaccagtaga tagcacaaaa ttcttataaa ttgctgtttc
     3181 ccataaaagc tccccagtct ttttcaaaac ttcatcaatg ctaccgtttt tcataacacc
     3241 agctgggtca acattcccca tagctactct tccccaaggt attttaggca tgatccttgc
     3301 catgtcaaca gcatttccga agtgcaatcc cattgctcct gttgaaacca tggactctac
     3361 ctgattttcc gtgttaccgc agttatgcag gatagccatg aagaattcat cctgaaccgc
     3421 ttcaacaatt tttctaacat attctgagga aaactcatgg cagtgttgtg gagacagcaa
     3481 gcctgctgct ggttctgcga taatgatgcc gtttgctccg gcatttttaa atgctttcgc
     3541 atattctata aggaactgag tggttttttc taatagcagc ttcattccat ccggctcaac
     3601 cattatattc accataatct cagtcatgtc aaacaaacgg cctgctaaag aaaatggccc
     3661 gatcatacct gcaagggtag gtctttcttt caggttcttt gctgccagtt ctacagcctt
     3721 tatatattca agcgtacgcc ctgcaccaat ctccggaaca ttcagttctt ccacttcttc
     3781 tatatcatta atggcaccct tgataacagt aggaacttca ttttctacaa atttgatctc
     3841 gcatccaaag gcctgcgctt cgacagacaa atccatgcag gcaacattcg ctattgaagg
     3901 gtatttctct gtaattgctt ttatgcactc atattgtgcc tttgcttcat ttactacctg
     3961 tataatattt atacttttca tttccgctcc gggaaaagac agtattggca ttgcctttct
     4021 atctggtgaa tttagtaaat tgtatatcca tgttttcata tttctcttca tattctattc
     4081 tcctttctac ttttggtttt cagttattac aactaaaaaa ccccttaact caaagttaag
     4141 aggcatttga acacaagaaa tttgtaagta gcttcatcat atcacctctc tatcgctcgt
     4201 agagtttaat ggtgcaacaa aacaggcagg tcttctggct taagcatcat cgtcagtgca
     4261 gcctttccgg tttcccagtg gcatcttgca aggattcctc tttcacagcg gcgggaccgc
     4321 gtgggatttc tacccacttc ccttttcacc aattcatgca tttgtccacc aaaaacataa
     4381 ttggtacctg tttccgacgt ttaattttca tctttactat atctcagcaa aacaaccttt
     4441 gtcatgtaaa ttttctattt ttttataaat attttattcc aattaaaaaa ttttggttac
     4501 taaacggggg acactcctgt attgtttttt aatgtccgct atttacatta ttttcatttt
     4561 atggtataat gtatattgag gtgataaata atggcaaggc ccaatagaaa aaaatctccg
     4621 gaaggtttgt accacgtaat gtcaagaagc attagtgaaa taaatcttta tcaaactgat
     4681 gaagacaaag atcattatct tcaactcctt aagcgctata aagaagaata ccattgcagt
     4741 atttactcat atatcctcat ggacactcat gttcatatat atataaatac ttgcggtgcc
     4801 gatatttcta aatttatgct ttgtctcaac actgcctatg taatatatta taacagaaaa
     4861 catcaacggc atgggcatct atttggtggc aggtttgcaa gcttcatagt tgataacgag
     4921 acctatagcc ttactctttc tgcatatatc cacaacaatg caaaggactt gccagggtat
     4981 agagggaaag aggaattata tccttattca tcatacggta tatataccgg tcacagagat
     5041 aatactgaag gcattgtgga tactgactat ttactaaagc attttagcag caacaaaaaa
     5101 gccgctcgca aaagctatag ggctttttca gaatccatgt gtggcacagg cattatgaag
     5161 gagatagatg acaatataat cagggcttac acagaaaaca attacaggag tgagaaaagt
     5221 tacattgtga gaaatgaaat accagatgaa ctattagcta agattcagga gatttttgat
     5281 aagaagatat cagcgggatt acttgcaccc gaaggtttaa cagaagaatt aactttaggt
     5341 ttatcagtag ggctgaaagc aggattaaga ttaaagtaca gtagaggaac gagcaatatt
     5401 agggcattta taacatacac tttaagaatc ttatgcggat actcttataa gcaattatgt
     5461 gaatatatag ggaacatgtc tttgtcaggt atttcaaagc tttctaataa gggattcagg
     5521 ttatttaagg aacatacgtt ataccagaat gcctttaatg ccttgatagg ggcaatatgg
     5581 cgctagattt aattttattt gggacaacat aatatcaatt cttataacat agtgtaacat
     5641 agtgtgatca ttatatctaa acataatagc aataataagg ttcagcaatg agctctctat
     5701 ttacaagcct tgtacaaagt attaaggtct ttttgctatt gtacttactt ctgtattttt
     5761 caggataaca gtatattatc taaatattaa ctgaatatat ctgaataatg ctctctgaat
     5821 aatgctcaag atatttcata atgaaggaag gaatgtccca aactatctat taaatccttt
     5881 tctctgcacc actgagcctg actttttcca cttgctttga attcttcaat tagcttgtcc
     5941 cagcctttat atgacatgag acacctcctg gttttttgta aattatctca tgtcggcgct
     6001 ttaattaaaa gacgggtttt attatacgct tacatacttc tggaaaagtg caaccacctt
     6061 cataaatatg aaatctcttc gtccattgca tacattgtgt ttcgtacctg gcaaaaaaca
     6121 caacaccaaa acctgaagcc cgcttaaatg gctaactttc tttctgttgt attatctgcc
     6181 aggctgcgta ctaaagctct ccgcttgcca ttggtacata agggtattac tccaatgaga
     6241 tgctttcaaa catatcggtc agattagcct caacgatatt tcaattagtg ggttagtcct
     6301 ccgtgtatgg tgacacatca ggctcaatcc aaaccttgcc gtcgcgccag tcaacatcga
     6361 agtcgaacag cttcgctatg tcgcgcagtt tggtatagtt gttgccgccg atattgtacg
     6421 cagtcaagtc tgccacatta ccgacccact ggccccattt gtcaaataac ataaactcgg
     6481 tgttgctggg tattgcactc ttgctgtctg tgccgattgc gacaagctcg ccgccgacag
     6541 gtgagtatgc cgcgtggtta tacagcttca ctttgccgtc ctcccatcgt acatcgaacc
     6601 gcgttttcag taacgccccc acgtcgcgca gtttgacgta gttattgctg cttatattgt
     6661 atgctgggag tgcaacttca gcgccgttta gcataaattt ggtgtccatc ggcttggcga
     6721 tgagccctgc gggagcagac ggctgctttg ccggcgcggc aggtggtatc ggtgcgggtg
     6781 caggtgcggg catcgatgaa atctcgtagt gactgtcacc aaagaatata tagccgtttt
     6841 caatgcccac accttggttc agaagattga agttattgct ctcagctttg tcaataacaa
     6901 tattgccact tcggtcaaag acagtccagt aacaagaata cacatcttca ggcagttgat
     6961 gcatagccaa cgtatagcct ccgacaatag gcaaggcata gacgtaacct tcgagttttt
     7021 taatgagctt ccccgaatag tcgtataatg aggagaagga ccaactatgt ccaccacctt
     7081 tggtgggtac tcctttggcg tttacctctt tagtaggagt tacggcgatg acaccatccc
     7141 tccaaagaag accgaagttg cttgaatgga accattcatc tttgttagcg ggaataatga
     7201 ctttgcccgt cttatctatt atgccggcca taggaagcgt aaacgggtct ttatccgttt
     7261 caacgaagta gcataccaag ccgtctttga aattatgcac attcccatca tagtaaaggt
     7321 caccatcaga catataggga ataaccaact taccggtggt gtcaatccat ccaactttgt
     7381 tagtgtcaac attacccatc ttaccttgaa atttgtacac tgctgccata tcattttgaa
     7441 aggaattagc ctcatcaaat tgcagaggta tgacttcttt tcctgtagtg tcaataaaac
     7501 ctgccttcat ggaatagtta aatgttgcgc cgcttttcat tacaacgata gttttgcctt
     7561 tttcgtccac ccagtaatca agacgttctc cattcataag agcgctagtt atgtcgtccc
     7621 cccacgttcc tactctcata agaccgtctc taacggtatg gtcacatata gaaccataat
     7681 acttggatgg cgtaatgaac ttgcctgtgg tgtcgatata ggcaaacaaa tctttgtctt
     7741 ttgctcgtac acgagctatt ccttgtgaaa agtttcttgc ctcttcaaac tgggcgggaa
     7801 tcaccacttt gccattttga tccttgtaac caattaatcc gttttctttg taagttttcc
     7861 agctctcacc ggtggtatct ggggtttcac ttgtgtatgc ggtaataaaa cggaattcac
     7921 cgtgttcggg agcgttatac caaccatatt tagctttatg tgtccatatt ataattggag
     7981 ttccgtcagc atttttttct cccgatgcat tgacaagcat tgtggaaact tcaggaggac
     8041 gtaaactgaa aatatccgtc ttttccgtat cgaaattagt cgattccgca taaatattcc
     8101 aagtataggg cttacttact gctattaccc gcataccttc ttttctcgta ccctcaagcc
     8161 ccaaatatct gccgtctttc attttcaggg taaatgcacc tccaccttta ctttccacat
     8221 aaaaggcttc atttttggaa agtttgcgga gttctgcctt cccgtccgca gtgatattga
     8281 ggtaattgtc catacagcgg agataatacc aatcgttttt aatagttgcc gctccccccg
     8341 ccgcaaaggc cgtcattggc agcagggtaa gagccacgca taagataagc agaatgctca
     8401 ttaatcgttt tttcataatg gttttcctcc tttagaaatg tttcttctca tctttaagtt
     8461 atcagaaatt tcacgaaata gacatcctct gcatgaaaca tcgttttttc gacatgaatc
     8521 atagttttgg atatctagtt tttatgttaa acttattatg tagaatacgc cgtctttgtt
     8581 gtttacctat taaacgtttt cgcaaattat ctgtcagatc tacatccata gggcatattt
     8641 ttttgcattt tccaaaagat acacatttat ctacatcatt atcaggctta ccagttgttt
     8701 ttgagcttga aaaggtattc gtctttggtt aactcataat ccactgatga ctgcaattca
     8761 cccaactgat ttttccatga attatagttt acagtgactt tacggaagcc aagcttttca
     8821 tacacatgct gtgcacgtat attattcaga ttgcaggaga gctaaagtat agtcaccctc
     8881 ctgtttattt ctagatttat caacactgta tttttcatat gataccattc ctttcgtttt
     8941 ggattttatt ataacgctgt agcggtataa cctttttgcc tagcttttac attttcagcc
     9001 cagctgtcat taaaattggt tgccttgcct atcgagtgaa ggtatcgaac aataccatta
     9061 aaattatgtt caaaaggttt tttctctttg tgaatagcta gaatatcatt tgtcttggca
     9121 acattattcg cttttatata tgcacataag ccttcagact acaatctcgg ctctatttta
     9181 gccgaatttt caaagtactc tgcccattca ttccacgcag cttccgttaa atctgcagga
     9241 ttttcaatag gtgcataagg tgtaaaagca gtatatgtca ctgtaagctt gaatcccagt
     9301 gcggaagaac cttaaagtat tcatccagat ttaatttata catatattcc tcctgttcgt
     9361 tataatttgt tcttattttg cctacttaac tgagcgatag acttgtttcg tttcgattta
     9421 tcaagcaaaa aacctgcttt gtcatctgca aactttgcct ctcgcttgat ttgaaggtaa
     9481 ctgctccaac gttccattga cagctctcca ttggccatgg cttctttgat ggcacagccc
     9541 ggctcacttt tatgctgaca atcggaaaac ctgcatttac caaaatagct ttccacatcg
     9601 gtgaacgctt cacccaagcc tgtgctcacc tcccacattc caagctcgcg catgccggga
     9661 gtatctataa tcataacacc gcttgaaagc ataatgagct ggcggtgcgt tgttgtatga
     9721 cggccgcggc tgtcctcctc ccggatttca tttaccatca taatttcctc accggcaagc
     9781 gcattgacaa gactcgattt accaacaccg gaggagccaa gaaagactat tgttttatgt
     9841 ggcttaagat attcggaaag cctatcaatg ccataccctg tctttgcact aacagcataa
     9901 acaccgacat cagctgctat cttttctaca gcccgggctt gttcagtaaa ttcgtccgtt
     9961 aaatctgctt tagttagtac aacaacagga attgcaccgc tttgccatgc tagagtgagg
    10021 taacgctcaa ttcttttaag attgaaatcg tggttgagtg atgccataat aaacacatag
    10081 tcaaaatttg aagctacaac ctgctctttt acagttttaa catagccgac tgcatgccct
    10141 gaaaaatcat tgcgtgcaaa ctttgactta cgttctaatg tttttattat ttgactgtca
    10201 ccactacaat tatattgaat taatacaaaa tccccaactg taggaaagct ttcctcacat
    10261 tctccgaaat atatgcttgt ttttagtctt ccaaaaacct gaccatattc acagatgatt
    10321 tcataacgct ccttgtgcac tgccgtaatg cgtgccgggg taccacttac gtcttccggc
    10381 atcattgttg ggataaatcc ataatctgct aaattcaact ttccataatt ttttatatcc
    10441 attattcaaa tccatccttt attaatttct tatctttctg cctgcgtttc tttttgttct
    10501 tcatttcatt ttcctgtacc ctggttcgag ggtttatcgc ccaaccacca cgaatttcct
    10561 ttatatattc aaaagtacct ttcatttttg tcatactcat cattctcccc atattcaaaa
    10621 caattagatt cctcaacctg aaaaagcata taaaaagccc gcagataacg accacctgcg
    10681 ggttcaaaaa gagacattcg tcctactgat gtgtgtccct tcaacttgta tgaaagcaac
    10741 aaaaaaacac gctaccaatg cgtgttgcta cttgttatat acacgattca gggccggtcg
    10801 caagctttgc ttgctgctcg cccatgccac ccggttgctc gaaagaagct gcataggcaa
    10861 cacaaacaaa acctaattaa taatgggaca ttccttgact taaaaggaat acaacatgtt
    10921 caaaaaggtg tcccataacc gcttacggta ttcctgttca cctgtgccga tattaagtta
    10981 gttaaaaatc acctcaacat tttttacaga catcaaaaca actcctttct caccaaaatc
    11041 taatacttgc agtatactgc atcaatttta ttatatgggt gattccaaac attgtcaacg
    11101 gagattttaa tttatagttg ttttatccaa aatgtccatg aaggtgaagc accaaatact
    11161 caactggcat tttggctttt tagctttttg tctttttggc tttttgttat attggttaga
    11221 gttcctgatc agtatcaatt acttccacta attcagataa tgctcttgtg aatgctatat
    11281 atttttcatt cttgctcata agaggtatcc cattcacagt taaaaagaga ccagtcatgc
    11341 caagcaatta tgtttaaaca tgactggtct cttttattac gacttagaac aattaaacta
    11401 tgagccaaat caaaacatcg tactagtaag gttcgctaat atcaggatgc ctttcttgct
    11461 taattattaa atacaacaat agtcgagagt gattttttct gttcttctgt aggaagcttc
    11521 agcttgccgt tgttgacaag ctctttcaag caatgcagaa tgaaccagcc atcggaataa
    11581 aaaatgtatt gcattccata agcctgcatt tttcgaagct gcttcggtgt agaatccata
    11641 acagctttga cgaatggtgc tttcagcgaa tcaaactctg ccttgtgcat tcccttgatt
    11701 ttgtcgccga cgtcaaggat tttttgtttt gtttcagctg agcgtatcca cacaacttga
    11761 gaagctgcaa agtgttctcc gttatattgt ccgtatgtcc ttatatagcc acgttcagct
    11821 aaataggcat actcatcttc ggaaagtcta tcctcttcaa agtggcgctg caacaaattc
    11881 aggtcccgta ctatccaatt ttggtaattg tcatccactc tttttgccga ccatatggta
    11941 tccaatcctt tctctaaatc tttttttgct tcgaacaaat gccatttgac tgtacagaga
    12001 ggaacttcaa gtgcttttgt aattcatcga tgtaagcaat aacttcatct gcatccttac
    12061 cttctacttg ttcaactaat gtagatgaat ctttcgggtc aatataagtt ccatcaacag
    12121 aagtaagaat aagtaagtat tttggatgga ctaacgtagc aattactgaa gctgtctcat
    12181 cgttgtcaat acattccaca attcctttat gacctttgct gcgaagctgt tctaattccc
    12241 agcgacggtt ttcttcgaaa ctaacggtat cattgtaatt tacgctatta tatgtattta
    12301 tattattgat gtaaagtaat atgaggcact tgggtgcata ttttaaaagt gaagaaatat
    12361 ttaccaggag ctgatgttac tctgcttaga gcctttaaat taagctcctg gcaaaattga
    12421 actcataaaa ttgtctgaaa gaatattaag taacaatttt actcaaacaa caattttgct
    12481 cttccttcgc tcagatatgg ggaaactctc tagcgtaaaa ggaatgcctg atacttgaga
    12541 gaagagtttc ccctgcccag ctacagcagc ttcacttatg ctccttacca atattttctt
    12601 cacttaaatt aaatgcaccc taagcacttt gcttgaactt cgacacagca attatacttc
    12661 ccggaaggca ttaacaacaa ataaaaagac tcttcaatcc ccataaaggg acgaaagagt
    12721 tgtccatggt accaacctaa ttagcttaat gcttacttaa ccagtactaa catactgtgc
    12781 ctatgtaacg taaggttgat tttttggata ttatgtagtt gcctgaaata taaaccagcc
    12841 atccttgact gaaaactgaa atacaccgcc atgcttttct actatataag ccatgctttt
    12901 tgtgccaaag ccatgcccct gctcttttga tacgggcagg ccctgatgaa atat
//
LOCUS       DAOSDD010000120        12924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1876_length_12924_cov_4.853835, whole genome shotgun sequence.
ACCESSION   DAOSDD010000120 DAOSDD010000000
VERSION     DAOSDD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_12924_cov_4.853835"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1177
                     /locus_tag="PK733_11285"
     CDS             2..1177
                     /locus_tag="PK733_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOJ11162.1"
                     /translation="MDKIVAAGNYPAKVIATKDRAEALKDADGVVCTILAGGVHVWRY
                     DVEIPKKYGVDINVGDTRGPAGIFRALRTIPVMLDICRDIETYCPNAIFLNYTNPMAM
                     LCRAMQGESKVQLTGLCHSVQGTAEMLARWIGAPMDEVTYLCAGINHQAWYLNFKWNG
                     KDAYPLIREAVENPEIYNVEQVRNEMFLHLDYYVTESSGHNSEYNAWFRKRPDLIEKY
                     CTHGTGWNPGEYAYILNCYLDREDNWKKDILDWLSKDEVNLKRGHEYAASIFNATIGD
                     GTMFEFNGNVRNFGLIDNLPEGCCVEVPILASKRGLDPIHVGPLPDHLAILNNISARC
                     EELAVEAAITGDPRKVFHAVCFDPLTSAVLSLAEIKQMVDEMFEANKDWLPQFKYLK"
     gene            complement(1288..2109)
                     /locus_tag="PK733_11290"
     CDS             complement(1288..2109)
                     /locus_tag="PK733_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11163.1"
                     /translation="MLEKYYFNQYNHTDLNVYRCGMEDCKPGHFWGPALRDHYIIHYI
                     SGGKGIYQVNGVTYNLVKGDGFLICPYTIVYYKADLEDPWSYAWVGFHGLKAESCLNQ
                     AVLTANNPIFRYNKDDFLLDCFSQMIEAMHLTRGRESRLIGLLYILLSQLMESANSVN
                     RQSKGENFKEEYIKKSIEYISMNYSRKISIKEIASHVGLDRSYLYSLFKEYLSSSPQD
                     FLINFRINKACDLMGNSSLSIGDIARSVGYEDPLLFSKVFKKVKNAPPREYRRGV"
     gene            2336..3304
                     /gene="galT"
                     /locus_tag="PK733_11295"
     CDS             2336..3304
                     /gene="galT"
                     /locus_tag="PK733_11295"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOJ11164.1"
                     /translation="MAELRWHPLIKDWVIIASHRQNRPQMPKDWCPFCPGSGKVPDNF
                     DVLKYDNDFPSLSQNPSIPDNVPTKFYKMKEAYGKCEVILYSPQHTITLPQLPVEHVK
                     KLVDLWTERFIELRKDEKIKYIFIFENRGELVGVTMPHPHGQIYGYSYIPKKLELELD
                     SCKEYYDQNNSCLICDMTRDEMEFKERIIFESEHFVVFLPFFVEYPYGIYITSKKHIQ
                     NLAQFTESEKLDLARTVRGAAGTLDSLFGFQFPYMMCMHQEPVNSGDFSKYYHFHIEF
                     FPPLRASNKQKFNASSETGAWAHANPTKPEEKAQELREAYKKFMSR"
     gene            3432..4622
                     /locus_tag="PK733_11300"
     CDS             3432..4622
                     /locus_tag="PK733_11300"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOJ11165.1"
                     /translation="MNTDQLKQKFLEIFGGPEEGIRVFAAPGRVNLIGEHTDYNGGYV
                     FPAALTMNTTIIVRSRKDRIIKLAATDLDDRVEASLGRLEDYKRIKWGNYQLGVADQL
                     QKNGYKLTGCDMLYHGTIPYGAGLSSSASIEVSTALALATIGNEAFSMNRDIGLIEMA
                     KIGQKAENIYVGVNCGIMDQFASAMGKANHAILLNARDLSYELVPLDLKDSKIVITNT
                     NKKRSLTDSKYNERRRECEDGLEMLKQAFPDANFLGEICYEKYKERQYLIKDEVIKKR
                     VQHVMSENNRVLKSVEALNKGDLCEFGNLMVQSHNSLRDFYEVTGLELDTLVEEALKI
                     DGVVGSRMTGAGFGGCTVSIVKEDAIDKFIENVGKGYSERVGLKASFYISETGEGARE
                     IIKL"
     gene            4652..5638
                     /gene="galE"
                     /locus_tag="PK733_11305"
     CDS             4652..5638
                     /gene="galE"
                     /locus_tag="PK733_11305"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOJ11166.1"
                     /translation="MAVLVTGGAGYIGSHTVLELLEASEDVVVLDSLEKGHRDAVTGG
                     RFYCGDLRDEKLLDSVFNENEIEAVIHFAAYSLVGESTGDPLKYYNNNVVSTLKLLTK
                     MKEYGVGKIVFSSTAATYGEPECIPISEQSRTSPTNPYGETKLAVEKVLKWADSAYGI
                     KYASLRYFNAAGAHISGKIGEDHSPESHLIPLVLQVALGKRDNITIFGSDYDTFDGTC
                     IRDYIHVTDLAQAHILALNQLRDGGNSVIYNLGNGQGFSVKEVINIAKEVTGVNINTV
                     MGDRRPGDPAVLVASSDKIKNDLKWKPEYNQLHTIIETAWKWHKTHPNGYDK"
     gene            complement(5681..6010)
                     /locus_tag="PK733_11310"
     CDS             complement(5681..6010)
                     /locus_tag="PK733_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11167.1"
                     /translation="MLGERLRQLREENKLSQKELAKYLGVTGRTISYYEYGKRFPSPE
                     TLNRIADYFNVSLDWLFGRTNIRTPIAKVAEKNSNREYNDPYSNNHEYYISEKELLEF
                     LQQKKKG"
     gene            6179..6418
                     /locus_tag="PK733_11315"
     CDS             6179..6418
                     /locus_tag="PK733_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11168.1"
                     /translation="MNYNQNFRLKEYRKMHKMTQKDLARLVNVSSDYISQLERGRRPG
                     MVTAIKIARIFDTTLENVFFQKIVDSDFTQHKVNK"
     gene            6601..7476
                     /locus_tag="PK733_11320"
     CDS             6601..7476
                     /locus_tag="PK733_11320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ11169.1"
                     /translation="MLVASDWKDFELIDAGNGEKLERWGKYILRRPDPQAIWPVAGDP
                     DNWEKVHAHYHRSRSGGGSWEFFSKLPEDWSIKYKELSFNVHPTGFKHTGLFPEQAAN
                     WDWIIEKIKNRIELGSGSVKVLNLFAYTGGATVAAAYAGAEVCHVDAAKGMVLRAKAN
                     LELSGLGHRTVRFITDDVIKFTQREKRREHFYDAIIMDPPSYGRGPDGEMWKLEDNFY
                     DLIQLCQEVLSENPLFFLINTYTTGLSPVVLKNILELTIKSKHGGRVSSDEIGLPISS
                     TGLILPCGAFGRWER"
     gene            7480..8325
                     /locus_tag="PK733_11325"
     CDS             7480..8325
                     /locus_tag="PK733_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOJ11170.1"
                     /translation="MRQNNEQINRQNDGFINKKKYGQARIKVIFEDNHLLVVEKPVNM
                     LSQGDSTQDRDLLTILKEDIKERYNKPGNVFLGLVHRLDRPVGGVMVFAKTSKAASRL
                     SEQIRMGEFDKKYLAVVNGKPASEKARLEHFLIKDEKTNMVKVLDNGSIILSNDNEII
                     RNDDKRIVEGNIKGIKGNKDAKKAVLDYQVLETGDKLSLVYVNLITGRSHQIRAQFSA
                     SGCPLYGDQKYGVELNKKGQQLALWSYKITLKHPVGKHEMSFQCNPPDAFPWNIFNSG
                     YLILT"
     gene            complement(8443..9279)
                     /locus_tag="PK733_11330"
     CDS             complement(8443..9279)
                     /locus_tag="PK733_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ11171.1"
                     /translation="MLKKGIGQSLVFFILFIVVMIMLFKANALYGLAFILFSLVAFCI
                     IKRAAIFAFLGRTSYYKGDMEKGLRWFKKAYKSGSAKPQTITSYAYLLLKTAKIQEAE
                     DILESLLRTKLEANDRMMVRSNMALVQWKKGNLDEAIQTLEEVITSYETTNIYGSLGY
                     ILIQKGDLDKALEFNLKASDYNSTNTIIMDNLGQTYYLRGEYDKAVEIYENLMDSNPS
                     FPEAYYNYALVLKEKNEIEKALETARKSLNYSLSFLSTIVKEDIDNLIKDLEEIAISN
                     QG"
     gene            9609..10289
                     /locus_tag="PK733_11335"
     CDS             9609..10289
                     /locus_tag="PK733_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOJ11172.1"
                     /translation="MKRSELNIIMQDAISFMETMNFKLPPFAYWSPDEWRNRGMEYDE
                     IRDNMLGWDITDFGSGNFRKTGLFLFTIRNGNSNSDKYSKPYAEKIMIVEEEQVTPFH
                     FHWNKMEDIINRGGGNLLIQLYNPTKDGKFSNEQVRVSVDGRNYYVDAGSIIRITPGE
                     SISLPLRQYHKFWGEKGAGKVLVGEVSKVNDDRTDNRFYEDIGRFPEIEEDEPPLYLL
                     CNEYPPAY"
     gene            10567..11520
                     /locus_tag="PK733_11340"
     CDS             10567..11520
                     /locus_tag="PK733_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ11173.1"
                     /translation="MKPRERFIAALERKPLKGLVPHFELVFFLTMEAFGKIHPSNRYY
                     SQWGQMSEKERQLHRNDIADIFITTAERYNHSAIFLHPNPWQLDEILRQIDLVREKTN
                     DKYFLMIHGDATFSIPDGESMQEFSYRLADEPEKLKEEAERLVDNALIKAEKIHKHGG
                     LDGFALCSDYCLNTGPFLSPNMFSEFVTPYLTKLIKGYKEMGFYTIKHTDGNIMPILD
                     QLIETNPHALHSIDPQAGVDIAEVKKMAGDKLCLIGNVNCGLLQTGTEDEVIESVRYA
                     LENGMPGGGYIFSTSNCIYTGMELERYELMLDIWKQEGIYK"
     gene            11630..12919
                     /locus_tag="PK733_11345"
     CDS             11630..12919
                     /locus_tag="PK733_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11174.1"
                     /translation="MSTSKKTEIPLYMQIKNSIIDQIEKGELQPGDMLLPELELCKVY
                     DASRFPVRQAMMELVKEGYLQRTRGRGTFVSKELSQVNSGSENINSQKTNAQNTSPEN
                     INSKNINSKNMNSDYRNPEYGNNRINNAEHKNLGCNCFKNKNPILGLVLGNYTSGFTS
                     SIFSGFQKQAWKRGYFTIACYSENSSDEESLCIDRLIENQVDGIVLFPCDKTLIGSKL
                     GKLKENNTYLGLIDRNPGLADVDYTGSDNRGGAYSAVRHIAMQGFRNVAFVSDMSNVS
                     SINERLEGYLKAVEDFGMNAVTRIDIQEDLSKYPYYMHRFFIEKLKDELMDLQKYLPL
                     GIFAINDGIAVQCVNILDERGLAIGKDVGIVGFDNNPETQYMNKPLTTVAQNGLLIGS
                     NAADIAADKIEGKANLVYRSIVPTQLIIRNSCGERQI"
BASE COUNT         4334 a         2088 c         2695 g         3807 t
ORIGIN      
        1 cgtggataag attgtggctg caggaaatta cccagccaaa gttattgcta caaaggacag
       61 ggcagaagca ttaaaggatg ccgatggagt tgtatgcaca attcttgccg gtggagttca
      121 tgtgtggcgc tatgatgttg agatacctaa aaaatacggg gtcgacatta atgtaggaga
      181 cacaagagga ccggccggaa tattcagagc acttagaaca atacctgtta tgcttgatat
      241 atgccgggac attgaaacat attgtcccaa tgctatattc cttaattata ccaacccaat
      301 ggccatgctt tgcagagcta tgcagggaga atcaaaagtt cagcttacag gtctctgcca
      361 tagtgtacag ggcacggctg aaatgcttgc cagatggata ggtgctccta tggatgaagt
      421 tacatatctc tgtgccggta tcaaccacca ggcctggtac ttgaatttta agtggaatgg
      481 aaaagatgca tatcctttaa taagggaagc cgttgagaat cctgaaatat acaatgtaga
      541 gcaggtaagg aatgaaatgt tcctccacct tgattactat gttacagaat ccagcggaca
      601 taattccgag tataatgcgt ggttcagaaa aagacccgat cttattgaaa agtattgcac
      661 ccatggaaca gggtggaatc ccggtgagta tgcctatatc cttaattgtt atcttgatag
      721 ggaagataac tggaaaaagg acatattgga ttggctcagc aaagatgaag ttaatcttaa
      781 gagaggacat gaatatgctg ccagcatttt taatgcaact attggggatg gaaccatgtt
      841 tgagtttaat ggaaacgtac gtaatttcgg acttattgac aacctgccgg aaggctgctg
      901 cgtagaggtt cctattcttg cttccaagag ggggcttgat ccaatacatg tgggaccatt
      961 accggatcat ctggctattc ttaataacat aagtgcaagg tgtgaagaat tggcagttga
     1021 ggctgcaatt accggggatc caaggaaggt tttccatgct gtatgctttg acccgctgac
     1081 ttctgcagtt ctgagcctgg cggaaattaa acagatggtc gatgaaatgt ttgaagcaaa
     1141 caaggattgg ctgccacagt ttaaatattt aaagtaatgt aaggtaatat aaggcaatat
     1201 aaggtattat aaagcgatat gaagtaatat aaagtaatat aaagcaatta aaattttatt
     1261 aagtagtaca tgcctcttta ttaagtatta cacgcctctc ctgtattctc tgggaggggc
     1321 attttttact ttcttaaaaa cttttgaaaa cagcagcgga tcttcatatc ccactgaacg
     1381 tgctatatca cctattgata gagaagaatt ccccataaga tcacaggctt tattgatccg
     1441 gaaattaatg agaaaatcct gtggtgaact ggatagatac tctttaaaaa gagaatataa
     1501 atagcttctg tctaaaccaa catgggaggc aatttctttg atactgattt tccttgaata
     1561 attcatggaa atatattcta tagacttctt tatatactct tctttgaagt tttccccttt
     1621 gctttgcctg ttgaccgagt tggcagattc catgagttgt gaaagaagga tatataagag
     1681 tccaataagt ctggattctc ttcctcttgt aaggtgcatt gcctcaatca tttggctaaa
     1741 acaatctaaa aggaaatcgt ctttgttgta tctgaatata gggttattag cggtaaggac
     1801 agcctggtta aggcagcttt ccgcctttag cccgtgaaat ccaacccatg cataactcca
     1861 gggatcttcc agatcagctt tgtaatatac aatagtgtag gggcaaatta aaaacccatc
     1921 accttttacc aggttatatg ttaccccatt aacctgataa atgcctttcc cacctgaaat
     1981 gtaatgaata atgtaatgat ctcttagagc aggcccccag aaatggcctg gtttacagtc
     2041 ttccataccg caccggtaca catttaaatc tgtatgatta tattgattaa aataatactt
     2101 ttccagcatg tcacaccctc aaatcatcag ttactataaa acattatacc atatttaaat
     2161 aacatacttc tatttacttt cagttaatat agggtatgat attagtgaaa atgtttattg
     2221 gcaattatta ttggtaatta ttattggtaa ttattatcgg taagtatggt caaatgctgg
     2281 cattagaata tgtattttga caaatttgtg ctaaattaag gatggtgaat tatttatggc
     2341 tgaattaaga tggcaccctt taattaaaga ctgggttatt atagcatcac atcgtcagaa
     2401 ccgtccccag atgcctaagg attggtgtcc cttttgtccc ggttccggaa aagtgcctga
     2461 taactttgat gtactgaaat atgataatga ttttccgtca ttatctcaaa acccttcaat
     2521 tccagataat gtcccaacaa aattctataa aatgaaagaa gcatatggaa agtgtgaggt
     2581 tattctttat tcaccccaac ataccattac tttacctcag cttcctgtag agcatgtaaa
     2641 aaagctggta gacttgtgga ctgaacgatt tattgaattg aggaaagatg aaaaaatcaa
     2701 gtatattttt atatttgaaa acaggggtga acttgtaggt gtaacaatgc ctcatccaca
     2761 cgggcagatt tatggatact catatattcc taaaaaactg gaattggaac ttgattcatg
     2821 taaggaatac tatgatcaaa ataattcatg tcttatttgt gatatgacca gggatgaaat
     2881 ggaattcaaa gaaagaatta tatttgaaag tgagcatttt gttgtatttc tacctttttt
     2941 tgttgaatac ccctacggta tatatataac ttccaaaaag catattcaga atctggctca
     3001 atttacagaa agtgaaaagc ttgatttggc aaggacggta aggggcgctg ccggtaccct
     3061 tgattctctt tttggttttc agtttccata catgatgtgt atgcatcagg aacctgttaa
     3121 tagtggtgat tttagcaaat attatcattt tcacattgaa tttttcccgc ccttgcgagc
     3181 atctaataag caaaaattta atgcatcaag tgaaacaggt gcttgggcac atgccaatcc
     3241 tacaaaaccg gaagaaaaag ctcaggaatt aagggaggca tataagaagt ttatgagtag
     3301 gtgaaaaaaa ttgcaatttt gcagccaaat agttaaaata gattcataat cgtcagggtg
     3361 atacacagca cgaaaaatga attttcatta ctatttgtgt tatcaatgat aggtatgaaa
     3421 tggaggcttt tatgaacact gaccagctta aacaaaaatt tttagagata tttggcggtc
     3481 cggaagaagg aattcgcgtt tttgcagctc ccggccgtgt aaaccttatt ggtgaacata
     3541 ccgactataa tggcggatac gtatttcctg ctgcccttac aatgaataca accataattg
     3601 taagatccag gaaggacagg attataaaac ttgctgctac agatctggat gacagggttg
     3661 aggcttctct gggcaggttg gaagattaca aaagaataaa atggggtaat tatcagttgg
     3721 gtgtggctga ccagctccaa aaaaacggtt ataagttaac tggctgcgat atgctttatc
     3781 atggaacaat accctatggt gcaggacttt catcatctgc ttctattgaa gtatccacag
     3841 cgctggcatt ggcaaccata gggaatgagg cattttccat gaacagagac atcggtttaa
     3901 ttgaaatggc aaaaatcggt caaaaagctg aaaatattta tgtaggtgta aattgcggta
     3961 taatggatca gtttgcttct gccatgggaa aggccaacca tgcaatacta cttaatgcca
     4021 gggatttgag ctatgaactt gtacccttgg acttaaaaga cagcaaaata gtaataacaa
     4081 atacaaacaa gaaaagaagt cttaccgatt caaaatataa tgaaagaaga agggaatgcg
     4141 aagacggact tgagatgctg aaacaagctt ttcctgatgc gaattttcta ggggaaatat
     4201 gttatgaaaa gtataaggaa cggcagtacc ttataaagga tgaagtaatt aaaaaaaggg
     4261 ttcagcatgt aatgtccgaa aacaatagag tgctgaaatc tgttgaagcg ctgaataaag
     4321 gtgacttgtg tgaatttggc aatcttatgg ttcaatccca taattcctta agagattttt
     4381 atgaggttac gggtttggaa cttgatactc tggtagaaga agcattaaaa atcgatggtg
     4441 ttgttggttc aagaatgaca ggcgctggat ttggcggttg tacagtgagt attgtaaaag
     4501 aagatgcaat tgataagttt attgaaaatg ttggcaaagg atatagtgaa cgtgtcggtc
     4561 ttaaggcttc gttttatata tcagagacgg gtgagggcgc acgggagata ataaaactgt
     4621 gaaagatcaa tgagcaattg gagggaaaat aatggctgta ctggttaccg gaggtgccgg
     4681 ttatatagga agtcatactg tgcttgagtt attggaagct tctgaggatg ttgttgttct
     4741 agatagcctt gaaaaggggc atagagatgc agttacaggg ggtaggtttt actgcggaga
     4801 cttaagagat gagaagctcc tggactcagt atttaacgaa aatgaaattg aggctgtgat
     4861 tcattttgca gcttattctc ttgtgggtga gagtacggga gatccattaa aatattataa
     4921 taataatgtt gtttctacat taaaacttct tacaaaaatg aaggaatatg gggttgggaa
     4981 gatagtcttt tcatcaactg ccgccacata cggggaacct gaatgtatac caatttcgga
     5041 gcagagcagg acttctccta ccaatccgta tggtgaaaca aagctggcgg ttgagaaagt
     5101 tttaaagtgg gctgatagtg catacggaat aaaatatgcg tctctcagat actttaatgc
     5161 agcaggggcg catatatccg gaaagattgg agaagatcac agccctgaat ctcatttaat
     5221 cccattagtg ttgcaggttg cactggggaa aagagacaat ataactattt ttggaagtga
     5281 ctatgatacc tttgatggca catgcattag agattatatt cacgtaacag acttggctca
     5341 ggctcatatc ctggcattaa atcaattgag agacggcggt aatagtgtta tttacaactt
     5401 gggcaacgga caggggtttt ctgttaagga agttattaat atagcaaaag aagttaccgg
     5461 ggtaaacatc aacactgtta tgggagacag gagaccagga gatcctgcgg ttttggtagc
     5521 gtcatcagat aaaataaaga atgatttaaa gtggaaacct gaatacaatc aattgcatac
     5581 tatcatagaa actgcatgga agtggcacaa aactcatccg aatgggtatg ataaatagag
     5641 gaagaggggg taaagaatat cttatagata ttctcaaaaa ttaccctttc tttttttgtt
     5701 gtaaaaactc aagcaactct ttttcagata tgtaatattc atgattattt gaatatggat
     5761 cattatattc cctattgctg tttttttctg caacttttgc aataggagtc cgaatgttag
     5821 ttcttccaaa caaccaatca agtgaaacat taaaataatc tgctattcta tttaaagttt
     5881 caggggacgg aaatcttttt ccatattcgt aataacttat ggttcttcct gttaccccaa
     5941 gatatttagc taattccttt tgggacaatt tattctcttc tcttaattgt cgaagtcttt
     6001 ctccaagcaa aaaattcacc accctaaaaa aattatagca tataggccaa tctaaattga
     6061 aaaacggaac gtaaagttct tgactaagaa cgtaatgttc cgctatacta tatatagttt
     6121 cttatatatg cttttttatg ataagtttag ttaaaaaatg tattttggag gaggaattat
     6181 gaactataat cagaatttta ggttaaaaga atacagaaag atgcataaaa tgacacaaaa
     6241 agacctggca agacttgtga atgtatcaag cgactatatt tcacagcttg aaagaggcag
     6301 aagaccaggg atggtcactg caattaaaat agcaagaatt tttgatacaa ctcttgagaa
     6361 cgtttttttt caaaaaatag tagattcgga ttttacacaa cataaggtta acaagtaatt
     6421 tttcggtcaa atcaacatat tagttgctat acaatttaga aaaagtgtat tatattatat
     6481 aggtataatg cgggtataac gtggaaaagt aatgttgtgt tgtatttggt gcctgttgta
     6541 tttggtgccc ggcaccaaat acaacaggca ccataaataa ttttggagga aacaattttt
     6601 atgttggttg caagtgactg gaaagacttt gaattaatag atgcaggtaa tggagaaaag
     6661 ctggaacgat ggggaaaata cattttaaga aggcccgatc cgcaagcaat ctggcctgta
     6721 gctggtgacc ctgataattg ggagaaggta catgctcatt atcaccggag tcggagtggg
     6781 ggcggaagct gggagttttt ttcgaagttg ccggaagact ggtcaataaa gtataaggaa
     6841 cttagtttta atgttcatcc tacaggtttc aagcatacgg gccttttccc tgaacaggct
     6901 gcaaattggg actggattat tgagaaaata aaaaacagaa ttgaattggg ctctggttca
     6961 gtaaaggttc tcaacctgtt tgcatataca ggtggagcaa ctgtagcagc tgcctacgcg
     7021 ggagcagagg tatgccatgt tgatgcagcc aaaggcatgg tgttaagggc aaaggcaaac
     7081 ctggagcttt caggacttgg tcatcggacc gtcaggttta ttaccgatga tgttataaaa
     7141 tttacacaga gggaaaaaag aagagaacat ttttatgatg ctataataat ggatccgcct
     7201 tcttatggaa ggggtcctga tggagaaatg tggaaactgg aagataactt ctatgacctt
     7261 attcaattat gtcaggaggt cttatcagag aacccacttt tctttttgat taatacatat
     7321 actaccgggc tttcacctgt tgtgctaaaa aatatattgg agttaactat taaaagcaaa
     7381 catggaggaa gggtaagcag tgatgaaata ggattgccaa tatcttctac cggactgata
     7441 ctcccctgcg gagcttttgg aaggtgggaa cgataatcta tgagacaaaa taatgagcaa
     7501 attaacagac aaaatgatgg attcattaat aaaaaaaaat atggacaggc tcgtattaag
     7561 gtaatattcg aggacaatca tcttcttgtt gttgaaaaac cggttaatat gctttcccag
     7621 ggtgattcaa cgcaagacag agatctattg acaattctaa aagaagatat aaaagaaagg
     7681 tataataaac cgggaaatgt attccttggt cttgtacatc gtctggacag gcctgtgggc
     7741 ggcgtaatgg tatttgctaa aacttccaag gcagcatcaa gattatccga acagataaga
     7801 atgggagagt ttgataagaa atatcttgca gttgttaatg gaaaaccagc ttcagagaaa
     7861 gcacgcctgg aacacttttt aataaaagat gaaaaaacaa atatggttaa ggtattagat
     7921 aacggttcta ttatacttag taatgataac gagattataa gaaatgatga taaaagaatt
     7981 gtagaaggta atatcaaagg aattaaagga aataaagatg ccaagaaagc agtacttgat
     8041 taccaggtcc tggaaaccgg agataagtta agccttgtgt atgtaaacct aattacagga
     8101 aggtctcacc agatccgtgc ccagttttca gcatcgggct gccctttata tggtgatcaa
     8161 aaatacggcg ttgaattaaa taaaaaaggc cagcaactgg cattatggtc atacaaaata
     8221 actttaaaac atccggttgg aaagcatgaa atgtcttttc agtgcaatcc gccggacgct
     8281 tttccatgga acatatttaa ttcaggatat ttaattctga cttaatttag gatacttaac
     8341 tccgaatact caatactact atgccaattc gaaaagcgat gtattctggg ataatttttg
     8401 acagcataaa gtagtattaa tctggaatat ctaatctgaa tattaacctt ggtttgaaat
     8461 agctatttcc tctaaatcct tgataagatt gtcaatatct tctttaacaa tggtactcaa
     8521 gaaggaaaga ctataattta aagatttcct ggcggtttca agagcctttt caatttcatt
     8581 cttctcctta agaaccagtg cgtagttgta ataagcctca gggaatgaag gattagaatc
     8641 cataagattc tcatagatct caacggcctt atcatattct cctctcaaat aatatgtttg
     8701 tcccagatta tccattatta ttgtattcgt actgttgtag tcacttgcct taaggttgaa
     8761 ttcgagagcc ttatcaaggt cgcctttttg aataagaata tagccgagac tgccgtaaat
     8821 atttgtagtc tcgtagctgg taataacctc ttcaagagtt tgaatagctt catctaaatt
     8881 cccttttttc cattgaacta gagccatatt cgatctgacc atcatcctgt cgtttgcttc
     8941 aagttttgtg cgaagcaaac tctctaatat atcttctgcc tcttgtattt ttgctgtttt
     9001 caagagaaga taagcatatg aagtaattgt ttggggcttg gcagatccgg atttatatgc
     9061 ttttttaaac cacctaagtc ccttttccat atcaccctta taataactgg ttctgcctaa
     9121 aaaagcaaaa atagcggctc ttttaattat gcaaaatgcc accaaactaa acaatataaa
     9181 agctaatccg tatagggcat tagctttgaa aagcattatc attactacaa taaacaaaat
     9241 gaaaaaaact aaagattgac caattccttt tttcaacata actccccctt tggttcattt
     9301 acatgataac acccgtaaat actgctttta ttatagttta ttagctttag atggtcaact
     9361 ttttattagg aaaaggtgca tttaaaattt ctgaagaaat atattccaga agctatcgtt
     9421 atagtactta gagctttcac caatgcttct tccaataatt tcttcaaaaa taaaatatgc
     9481 acccttaggg gaatatgctc ttaagggaag tgtgccagga tatcttatat tcagaaaata
     9541 attgaaacaa tctatgttct atactacaat atatataata tacagaatat gacatggggt
     9601 gattctttgt gaaaaggtcg gagttgaaca taatcatgca agatgcaatt tcatttatgg
     9661 aaaccatgaa ttttaaactg cctccatttg cttactggag ccctgacgaa tggagaaaca
     9721 gaggcatgga gtatgatgaa ataagagata atatgctggg atgggatata accgatttcg
     9781 gaagcggaaa ttttagaaaa acaggacttt tcctatttac tatacgtaat ggaaattcga
     9841 atagtgataa gtattcaaag ccttatgcag agaagataat gattgttgaa gaagagcagg
     9901 ttaccccttt ccatttccac tggaacaaaa tggaagacat tatcaataga ggcggaggca
     9961 acttgttgat tcaattatac aaccctacca aggatggtaa attcagcaat gaacaagtaa
    10021 gggtttcagt tgatggaaga aattattacg tagatgcagg tagtattatt agaataaccc
    10081 ctggtgaaag catatccctg cctctgaggc agtatcacaa attctggggt gaaaagggag
    10141 cgggcaaggt actggttggc gaagtatcaa aagtaaatga tgacaggaca gataaccgct
    10201 tttatgagga tattggcaga ttccctgaga tcgaagaaga tgagccgccg ctatatcttc
    10261 tatgcaatga atatcctcct gcatattgaa cttaacttaa ggaacaagct tcagttatta
    10321 ctgaagcttg ttccttagat atgcctgctt taagcagctg gttttataga ccgttttata
    10381 tatggtgtgt ttttcatatt gacaataaca acaacataca atagaataaa catagataac
    10441 atataaataa caaaaacata accaatgtga taaaagcata aaacaaacat aacaaatgca
    10501 tgacaaatat caagcataac aagcgtaaat tgtatgcttt tcacactatt tatgggggcg
    10561 gatttaatga aacctagaga aagatttatt gctgctcttg aaaggaagcc tttaaaagga
    10621 ctggtacctc attttgaact tgtatttttc cttaccatgg aggcttttgg gaaaatacat
    10681 ccatcaaaca gatattacag ccagtggggt caaatgagcg aaaaggaaag gcagctgcat
    10741 agaaatgata tagcagatat ttttattaca actgcagaaa gatacaatca tagcgctatt
    10801 tttcttcatc caaatccatg gcagcttgat gaaatcctaa ggcaaattga cctggtaaga
    10861 gaaaaaacta atgataaata ctttttaatg attcacggag atgctacttt ctccatacca
    10921 gacggcgaaa gcatgcagga attcagttac agactggcag atgaacctga aaaattgaag
    10981 gaagaagctg aaaggcttgt ggataatgcg ctgattaaag ctgaaaagat acataaacat
    11041 ggtggtcttg atggatttgc cttgtgctct gattattgcc taaacactgg tcctttccta
    11101 agtccgaaca tgttttcgga atttgtaaca ccctacctga caaaacttat taaaggctat
    11161 aaagaaatgg gtttctacac aataaaacat actgatggaa atataatgcc catacttgac
    11221 cagcttatag aaaccaatcc gcatgctctt cattcaatag atccccaggc aggtgttgac
    11281 attgctgaag ttaaaaaaat ggcaggagat aaattatgtt taatagggaa tgttaattgc
    11341 ggacttctgc aaaccggaac agaggatgag gtcatagaat cggtgagata tgcactggag
    11401 aatggtatgc cgggaggagg atatattttc tcaacatcca actgcatata tactggaatg
    11461 gagcttgaaa ggtatgaact tatgcttgat atatggaaac aggaaggtat atataaataa
    11521 agataactaa ttcccgatgc tatcaaaaaa tatcccggaa ggcatggctt tccaaattag
    11581 catagtagta ttagtgtaat ttaatatcgt taggagaatt aaaagcatta tgagtacaag
    11641 caaaaaaacg gaaataccgt tatacatgca aataaaaaac tctataattg atcagataga
    11701 aaaaggggaa ttgcagcctg gagatatgct gttacctgaa cttgaactgt gcaaagtata
    11761 tgatgcaagc agattccctg taaggcaggc aatgatggaa cttgtaaaag aaggatatct
    11821 tcaaagaaca agaggcaggg gtacttttgt ttcaaaagag ctctcacagg ttaattcagg
    11881 tagtgaaaac attaattcac aaaaaacaaa tgcacaaaat actagtccag aaaatataaa
    11941 ttctaaaaat ataaattcta aaaatatgaa ttcagattat agaaaccctg aatatggcaa
    12001 caacagaatt aataatgctg aacataaaaa cctcggctgt aattgtttta aaaacaaaaa
    12061 ccctatacta ggtttggtat tgggtaatta cacttcaggg tttacctcct ctattttttc
    12121 agggtttcaa aaacaagcct ggaaaagggg atactttaca attgcctgtt actctgaaaa
    12181 ttcttctgac gaggagtcat tgtgtattga caggcttata gagaatcaag ttgatggaat
    12241 tgttcttttc ccatgtgata aaacgttaat tggctcaaaa ttagggaagt tgaaggaaaa
    12301 caacacatat ttggggttaa ttgatagaaa cccgggactt gctgatgttg attatacagg
    12361 ctctgacaac cggggtggtg cttattctgc agtgaggcat attgctatgc agggattcag
    12421 aaatgttgct tttgtttcag acatgtccaa tgtttcttca ataaatgaac gccttgaagg
    12481 atatctgaag gctgtagaag actttggaat gaatgctgtt actcgaattg atatacaaga
    12541 ggatttaagt aaatacccat attatatgca tagatttttt atagaaaagc taaaggatga
    12601 gcttatggat ttacagaagt accttcctct tggaatattt gctataaacg atggaattgc
    12661 agtacaatgc gtaaatattc ttgatgaaag ggggcttgcc attggaaagg acgtaggtat
    12721 agtaggcttt gataacaatc ctgaaacaca atatatgaac aaaccactga caacagttgc
    12781 acaaaatggg ttattgattg gcagcaatgc ggcagatatt gcagcagaca aaattgaagg
    12841 taaggcgaac cttgtgtata ggtcaattgt gcctactcag cttataatta gaaattcatg
    12901 cggggaaagg cagatttaag gata
//
LOCUS       DAOSDD010000121        12782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1897_length_12782_cov_5.060816, whole genome shotgun sequence.
ACCESSION   DAOSDD010000121 DAOSDD010000000
VERSION     DAOSDD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_12782_cov_5.060816"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK733_11350"
     CDS             complement(<1..351)
                     /locus_tag="PK733_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ11175.1"
                     /translation="MKFNSEMPRLGSKRILSKTLGILLTFCLVLSNFPILNNVSAEEP
                     QPELIQMPNMVVNSGFESDLASWTKVIQTATISIDDTVAYEGSKSLKITNTTASTRGC
                     IDQQYFTLDPGATYV"
     gene            complement(902..1774)
                     /locus_tag="PK733_11355"
     CDS             complement(902..1774)
                     /locus_tag="PK733_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOJ11176.1"
                     /translation="MTWSVIATWKFSLTGVAEAGKLVACGRKSLDAVEQVARYVEDDP
                     SVDTVGYGGIPNVAGEVELDAAIMNGEDLSIGAVMGVKGYRNPISIARKVLEDTPHNI
                     LVGQGAEDFAARKGFEKTMLLSEKSIETWFKMMNELKAGNKHIEGHDTVGVVALDTFG
                     RMASGTSTSGISMKYRGRVGDSPLVGSGFYVDDYVGGAAATGVGEDIMKGCTCFYAVE
                     LMREGCNPREAAELAVMRTHKRIFEKTGKVGNIAIVCADNKGNFGGAANHEGFEYAAA
                     SDKCLPKIYVVEPV"
     gene            complement(1931..2995)
                     /locus_tag="PK733_11360"
     CDS             complement(1931..2995)
                     /locus_tag="PK733_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11177.1"
                     /translation="MSNYCNNNLKLGVAYHGNRMLKHVRDDMLDIIQHHFNLVVHMFS
                     HNDWDRHKNIMKEIIEISEDAGLEVWVDNWGLGGPPGDKSHFLSYYPEAHQVYSNGEM
                     DPVRVCLNNKDFRKFTKEWIDVVDYSGGKSIFWDEPHLAGKEMENGKPKVWTCYCSTC
                     QKLFEEKYGKKMPSELTSEVEEFRIHSVVDYFREVTAYSKAKGMYNIVCVMLGDSYGI
                     NLATIDQISGLDTLDNIGSDPYWIGHQGVNPYEFVYNATKKNLDICTKFKKDHNLWIQ
                     CFNNPRGREEEIIYATDAAYDAGARTILAWGYRGSESNDYRAKNPDLTWKVTGDAMYR
                     LLERERNTNRDNYRKLVDCR"
     gene            complement(3080..4231)
                     /locus_tag="PK733_11365"
     CDS             complement(3080..4231)
                     /locus_tag="PK733_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11178.1"
                     /translation="MSQAYCDKNLIQFINPINGDVLNEFDGQIKGKSLEIKVKLLAGN
                     QSRVIVNGVPATIVNSLASKSDCCCSFSKGEGTLYEAAINLDGYRNTVVAVDENSGCK
                     NEMVVYWLKNATGKYRISMDDNIWFLKDIAKNSDKYKSIFENPYLKLYKDVHDMYGTK
                     VHINLFYQTEGFNLSQMPDKYKSEWKDNAHWIKLTFHSLQEFPDRPYANATYDEIKKD
                     YELITNEIIRFAGEELLSPVTTVHWGATSLEAARALRTMGMKGLVGYFKFAEDGAPLV
                     SYYHDNERIKHINKRDYWKDDKEDIMFVKHDIVLDGTNLKDICPFLDNLKKDPHQSGL
                     IEMLIHEQYFYPHYKAYQPEYREKVMTAARWATENGYKPAFWSDVVFEK"
     gene            complement(4409..5446)
                     /locus_tag="PK733_11370"
     CDS             complement(4409..5446)
                     /locus_tag="PK733_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ11179.1"
                     /translation="MERVVDFFYEKSKIEAHSGCMFKGRHYRCFYVTFPTLYKTPAPG
                     TETVQLYDFRPKDKIRASVVILHGLGTRNIEFLLWMGTHLASAGVNAVVPILPGNFTR
                     IEDGMIYGSRYLWPDINVMYDVYQHAIVDIRSTIDYLEQQNRWKDNNCMVGYCLGGML
                     TTIVSCLDERINQKILMTTGGNIPQLLFESSSTRFVRRLIAEGRVEDRTLNDKNILYE
                     IYDKQFPQIKQMSLEEIITSRDIHPLFKVDPLSYAHLLDKSKVSVIDALFDRTLSITS
                     RKSLFKEMEGAKRYVLPISHVNWLPFEFLLGRYILHKVNIYDRKTASRILTKEIVRDA
                     IEDEQEIWMKK"
     gene            complement(5448..6572)
                     /locus_tag="PK733_11375"
     CDS             complement(5448..6572)
                     /locus_tag="PK733_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ11180.1"
                     /translation="MLQKNIQKKIESLKNSVFIVNKLYLWVLFLLLAVTDWSLAFYSF
                     LSYIVIYLWNIFTARLPIENKGEFQYITKVYKTIEDSELKMDIWYPNQEKGKGDYPLV
                     LFAHGGGWVSGFRNQPNNISWCRYLAANGFVAASIDYRFGIKNDMEDILSDYDNALSY
                     LKSHAKELRICPHKIILMGLSAGGHLSLLYSSHYSNRRDKGRMKGICGVVAYYAPSDL
                     TDLFSPESRSLFARLATIQTLKGKPDSTLKGKPDKAFKGKYNKILKENLDGKLKRNLN
                     RKSNEKLDQQKNYEYYSPIMWISKRMPKVFLAHGKNDRTVPFSSSVKMAGKLEEFRVS
                     YKLMAHPKGDHCFEMNSKDLQTIRILRMTISWMREVIGEE"
     gene            complement(6594..8297)
                     /locus_tag="PK733_11380"
     CDS             complement(6594..8297)
                     /locus_tag="PK733_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ11181.1"
                     /translation="MKKLPYGISNYKMMVEENLYYVDKTMYIEELEKLHSYYLFFLRP
                     RRFGKSLFVSLLQNYYDINEKDNFDTLFRNTYIGQNPTKERNSYYVLNFDFTGVTTDT
                     KEQLEESFINITKNAFELFQNRHNIHFDYLQTGSAANIFEDFFTKVTTRIEKPIYVII
                     DEYDHFANELLSFQFDFFKESVSNTGFVRKWYEVLKKQTKTIVKRIFATGVSPITLDS
                     LTSGFNIADNITREEAFNEMMGFTEDEVRYMIKETVKFPIGRNEMDTLMEVLHKNYNG
                     YLFSEKAHKRIFNSDMILYYLKTYIEDKEGPIELIDQNIASDYGKLGKMFELKNRKGN
                     MEVLEKILNDEEIVAQITRQFSMEKDFTTDDFKSLLFYLGLLTIDERVLDSVSLKTPN
                     YIIKGLYYQYYAKKINEETGYDINTSFIKEGVRQIAIEGKNDKFISVIEKTLHKLSNR
                     DYINFDEKYIKLIMLSYLMLSNIYMVKSEYEVEDGYIDIALLRREPIEPSYFAIFEVK
                     YIKKMEYEIYGDKIVEGKKNEATKQIRKYHSSSELQSLSKLKKWVIVFVADRCVVNMD
                     I"
     gene            complement(8321..9682)
                     /gene="glmM"
                     /locus_tag="PK733_11385"
     CDS             complement(8321..9682)
                     /gene="glmM"
                     /locus_tag="PK733_11385"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ11182.1"
                     /translation="MGRLFGTDGVRGIANVELTPELAYKLGKAGAYVLTQETKHVPKI
                     LVGMDTRISGHMLKSAIVSGICSMGAEAVVLGVIPTPAIAYLTRYYGADAGIVISASH
                     NPFEFNGIKFFDGRGYKLPDHIEDSIEGIILDGREALPEIAGRGIGRENIVGSAAEDY
                     LAFIDDSIDVDLKGIRIAVDCANGASYRVAPEILKRLGAEIFITAKNPNGININNGCG
                     STYIENICSFTRKCGADIGLAFDGDADRLLACDEIGNEVDGDKILAITGLQMKKEGRL
                     AKNTIVVTVMTNIGFDVMAAREGINIVKTKVGDRYVLEEMLNNGYILGGEQSGHVIFL
                     DYNTTGDGLITALQLLRVMKVTGKKLSELAGVMEVFPQVLRNARVSNDNKYKYLEDQV
                     IADMCRHLEEELHGEGRVLIRPSGTEPLVRVMIEGKNLDYITNRAEELVRLIEIRMMK
                     INQ"
     gene            complement(9772..11136)
                     /locus_tag="PK733_11390"
     CDS             complement(9772..11136)
                     /locus_tag="PK733_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOJ11183.1"
                     /translation="MRYIINRLNNKLSEDLATKLISVFLGIILWFIVLNINNPYIEKK
                     FYIELDVRNENTLQEKNIYLVNKNYRRTVEVVVQGRQSTLDSLSSADFEAVLDFSKVR
                     SVNDKSIKVDDPYYTKNDKQITITGMSPKEIPIELEHVLKKELPVMIELKGTPKQSYM
                     VIKTATKPEYVSLHDRESLINTVSSVKAVVDINGIDRDKKVINQSCTVYNTKGEEISS
                     LSNQFTVDIVLEVGKEVPIEPVITGEPAADHVISGIKVNAPNAVITGSYDIISKITSL
                     STETINVDGLTAAKDYKVPIKLSEGVKLYNTEADATVAIEVEKLEEREIVISKMSINI
                     ENVAANMGNMGNNSDNGGSQLKYEILTDSCKVILKGRTASLNNINPSILTPHIDVEGL
                     QEGVHHVQLKVSPSPDIDVIKLPSVEVKITKVKNEKPPEENTNEDGRTDGKDSSNNTE
                     SITS"
     gene            complement(11133..11975)
                     /gene="cdaA"
                     /locus_tag="PK733_11395"
     CDS             complement(11133..11975)
                     /gene="cdaA"
                     /locus_tag="PK733_11395"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOJ11184.1"
                     /translation="MFNNFVGYVGITGPLSVIRAIVDISLVTYVIYKIIILVRETRAW
                     QLIKGIIFILVAAELSKLLKLNTIAYILSNTVSILALAMVVLFQPELRRGLEQIGRSR
                     FRDFFNFEDSEDKLRTTAMIEDIVKACIELSRNFIGALIVVERDTKIGEIIKTGIELN
                     SNISSELIINIFTPNTPLHDGAVVIRDNKLKAASCFLPLTDNPNLSKELGTRHRAALG
                     ITEVSDAIAVVVSEESGKISFALGGGLTRNLTPDTLRKALNKNLLENSPSNRKLTLWK
                     VKNK"
     gene            complement(12155..>12782)
                     /locus_tag="PK733_11400"
     CDS             complement(12155..>12782)
                     /locus_tag="PK733_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ11185.1"
                     /translation="TFGCNLKCSFCQNWTIAHDIPESFEITPSRLVQETEKNVSEGNI
                     GIAYTYNEPSIWYEFVYETSKLIKGKGLLNVLVTNGFINSEPLENLMPYIDAMNIDVK
                     AFNSSFYKDVCKGDGALEYIKSTIEQASQKCHLEVTTLVIPELNDSMDEIGELAKWLA
                     SISKDIVLHLSRYFPNYRMNKPATPLKTLNAAAEKASHYLRYVYLGNV"
BASE COUNT         3709 a         2736 c         1846 g         4491 t
ORIGIN      
        1 cacataggta gctcccggat caagggtaaa atactgctga tcgatacatc cccttgtact
       61 tgctgtagta ttggttattt ttaaggactt ggaaccttca taagcaactg tatcatcaat
      121 agaaattgtt gctgtttgga ttacttttgt ccaactggca aggtctgatt cgaaaccact
      181 attaacgacc atgtttggca tttgaataag ttccggctga ggttcttctg ctgatacgtt
      241 gtttaatatg ggaaaattac tcagcactaa acaaaaagtc aataaaatac ccagcgtctt
      301 tgaaaggatt cttttggatc ccagccgagg catttctgag ttaaacttca tattgctaat
      361 tcctcctctt ttgatttaaa taattgtttt cttattcttt tttcttttga cattatttta
      421 ggtgttataa ttaataacca aaatcgccaa aaaaattgtt gctttaatga ataaataacg
      481 ctagactgtc acctcccacc ctccagaata agaaatagaa cgatagagca aaaagagttt
      541 tcataatata taaaaattct acaaattgaa tatatattta aagatttttt ttgtaaagtg
      601 ccactttatg agtgctttaa caaatcatgc gcattacatg aatttattat atttatatgc
      661 tgatatatgc ttttggaaat atggctttaa ttgaattaaa ttgtatttga cgtgttaact
      721 atctgaatta taatgtttaa cattacattt gtatttaaca ttttattacc tttaagcaat
      781 ttaaaatgtg acaaagggtg catagtgtaa taaggtcagg ggacacacct ctctggaagt
      841 atagaaattt ataaagttcc taaaaaattc ttataccaag gtatgtccct gcttattttc
      901 gttacacagg ttcaactaca tatatcttag gcaagcattt atccgaagct gctgcatact
      961 caaatccctc atgatttgca gctccgccga aatttccttt attatccgca catacaatag
     1021 cgatattgcc tacctttccc gttttttcaa atattctttt atgagttctc attactgcca
     1081 gttctgcagc ttcccgggga ttacatcctt ctctcattaa ctctacagca taaaagcagg
     1141 tacagccttt cattatatcc tcacccacac ccgttgcagc ggctccgcct acatagtcat
     1201 caacataaaa tcctgaccct accaacgggc tgtctccaac cctgcctctg tatttcatac
     1261 ttatcccact ggttgaagtg ccgcttgcca tccttccaaa ggtatcaaga gcaacaacgc
     1321 ctacggtatc atgaccttca atatgcttgt ttcctgcttt taattcattc atcattttaa
     1381 accaggtttc aatgcttttt tcacttaaga gcattgtctt ttcaaagccc ttcctggcag
     1441 cgaaatcttc tgctccctgt cccacaagaa tattgtgagg agtgtcttca agaacctttc
     1501 tagctatact gattggattt ctatatcctt tcacacccat aacagcgcct attgaaaggt
     1561 cttcaccatt cattattgca gcatcaagct caacctcacc cgcaacattg ggtattccgc
     1621 cataacccac tgtatctact gaagggtcat cttcaacata tcttgcaacc tgctctacag
     1681 catccaggga tttcctcccg caagcgacca attttccagc ttcagctacc cctgttaatg
     1741 aaaacttcca tgtagcaata acagaccaag tcattgttaa tacctcccaa aataaatact
     1801 ttaataagca accgctttat atttcttatt caggcgttac aaatctgctt ccttgatgtt
     1861 gtggtctgct aactccaaaa ataatctgtt gcagaccaca aaacttttgt aatgtagttt
     1921 cttttgtacg ttatctacag tcaaccaatt ttctatagtt gtccctatta gtgtttcttt
     1981 ctctttcaag gagtctgtac attgcatccc ctgttacctt ccatgttaaa tcagggttct
     2041 ttgcacggta atcattggat tcagagcctc tatatcccca tgctagtata gtcctagcac
     2101 ctgcatcata ggcagcatcg gttgcgtaaa taatttcctc ttccctgccc ctcgggttgt
     2161 tgaaacattg aatccataag ttatggtcct ttttaaactt tgtgcaaata tccagattct
     2221 ttttagttgc attataaacg aattcataag gatttacacc ctgatgtcca atccagtatg
     2281 ggtctgaacc tatattatca agggtatcaa gtcctgatat ctggtctatt gttgccagat
     2341 taattccgta tgaatcgccc agcataacac atacaatatt atacatacct ttagctttac
     2401 tgtatgcagt aacttctctg aaataatcga ctacactatg aattctgaac tcttcaactt
     2461 cactcgtcaa ctctgaaggc atctttttcc catacttttc ttcaaagagc ttctgacagg
     2521 tgctgcaata acaggtccaa accttaggct tcccgttttc catttctttg cctgcaagat
     2581 ggggttcgtc ccagaatatg gattttcctc cgctgtaatc aactacatca atccattcct
     2641 tggtaaactt tctgaagtcc ttattgttaa ggcatactct tactggatcc atttcaccat
     2701 ttgaataaac ctggtgcgct tcaggataat atgaaagaaa atgggattta tcaccaggag
     2761 gccctcccag tccccagtta tcaacccaaa cttccaggcc tgcatcctcg gatatttcga
     2821 taatctcttt cataatattc ttatgtctat cccagtcatt atgggaaaac atatgaacaa
     2881 caaggttaaa atggtgttga ataatatcca gcatatcgtc tcttacatgc tttagcattc
     2941 tattgccatg atatgcaaca cccaatttaa gattgttatt acaataattg gacataattt
     3001 cacacatcct ttctcactta ataccaacta tttaaattta acttctcaat tcaaactttt
     3061 aattctaatt ttttatttct tatttctcaa aaactacatc actccagaat gcaggtttgt
     3121 aaccattttc agtagcccaa cgagccgcag tcatgacctt ttctctgtat tccggctgat
     3181 aagccttata atgaggatag aaatactgtt catgtatcag catctcaatt aagccgctct
     3241 gatgggggtc ttttttcaaa ttgtccagga aaggacatat atctttaaga ttggttccat
     3301 cgaggactat atcatgcttt acaaacatta tgtcttcttt gtcatctttc caataatccc
     3361 gcttattaat atgctttata cgctcattat cgtgataata tgataccaga ggtgccccat
     3421 cttcagcaaa cttaaaatat cctaccagcc ctttcatacc catagttctt aaagctctgg
     3481 cagcttccaa tgaagttgca ccccaatgca ccgttgttac agggcttagc aattcctctc
     3541 ctgcaaatct tatgatttca ttggttatca gttcgtaatc cttctttatc tcatcataag
     3601 ttgcatttgc atatggcctg tctggaaatt cctgcagtga atgaaatgta agctttatcc
     3661 agtgggcatt gtctttccat tcactcttat atttatcagg catctgagaa agattgaatc
     3721 cttcagtctg ataaaataaa tttatatgta ctttagtccc atacatatca tggacatctt
     3781 tatataactt caaatatggg ttttcgaata tggatttata cttgtcactg ttttttgcaa
     3841 tatccttaag aaaccagata ttatcatcca tagaaattct atatttaccg gtggcgtttt
     3901 tcagccaata tacaaccatt tcatttttgc agcctgaatt ttcatctaca gctactactg
     3961 tattcctata tccatccaga ttaattgcag cctcatacaa agttccttct ccctttgaaa
     4021 atgaacagca acagtcagat tttgaagcta agctatttac aattgttgct ggtactccat
     4081 taacaataac cctgctttga tttccggcca gaagctttac ctttatttca aggcttttcc
     4141 ctttaatctg tccatcaaat tcatttaata catcaccatt tatcgggttt atgaactgta
     4201 tcaggttctt atcacaatac gcttgtgaca ttaaaaatca ccgccttata agattatttg
     4261 agttaatatt ttaaaatgag tctaacataa ttaaataaat aataatcatt atactgattt
     4321 aataatttat ttactgccta aaataattat atcacaggtg ggactttttg caatatttta
     4381 tatatacttt tagcatgtac ttttgtattc actttttcat ccaaatttcc tgctcatctt
     4441 caatagcatc acgtacaatc tctttcgtaa gaattctgct tgctgttttg cggtcatata
     4501 tattaacttt atgtaaaata tacctgccta aaagaaattc gaaaggcagc cagtttacat
     4561 gggatatagg aagaacatag cgtttggcac cttccatttc cttaaataat gacttacggc
     4621 tggttattga taatgtacga tcaaacagtg catcaataac agataccttg gacttgtcca
     4681 gaagatgtgc atatgacaat ggatcaacct taaaaagtgg atgaatatcc cgggatgtta
     4741 ttatttcttc gagggacatc tgctttatct gcggaaattg tttatcatat atttcataaa
     4801 gtatattctt atcattcaga gttctatctt caactctccc ctctgctata agtcttcgta
     4861 caaaacgtgt tgaactagat tcaaacaata actgtggtat attgcctccc gtagtcatta
     4921 aaatcttctg attgatcctt tcatccagac aagacacaat agtggtcaac atgccgccca
     4981 aacaatagcc caccatacaa ttgttatcct tccagcgatt ttgctgttca agatagtcta
     5041 tagtggaacg aatatccact atagcgtgtt ggtatacatc atacataaca tttatatcag
     5101 gccataaata tcggctgcca tatatcatac catcttctat acgtgtgaaa ttgcccggca
     5161 gtattggtac tactgcattc acccccgctg atgccaagtg agtacccatc cacagcaaaa
     5221 actctatatt acgcgtgcca aggccatgta atataacaac agatgcccgt attttatcct
     5281 tgggcctaaa atcatatagc tgaactgtct cagtgcctgg cgccggtgtt ttgtataaag
     5341 taggaaatgt aacgtaaaag catctgtaat gccgtccttt gaacatgcac ccggaatgcg
     5401 cttctatttt ggatttttcg tagaaaaagt caaccactct ttccatgtca ttcctcccca
     5461 attacctctc tcatccagct tatagtcatc cttaatattc gaattgtttg taaatccttg
     5521 gaattcattt caaagcaatg atcacctttt gggtgcgcca tcaatttata tgataccctg
     5581 aattcttcca gtttaccagc cattttcaca gaagatgaga aaggtacagt acggtcattt
     5641 ttaccatggg ctaaaaatac tttaggcata cgttttgata tccacataat aggcgaatag
     5701 tattcataat tcttctgttg atcgagcttt tcatttgatt ttctattaag atttctcttt
     5761 aactttccat caagattttc ctttaatatc ttattatatt tccctttaaa tgctttatca
     5821 ggctttcctt ttaatgtgct atccggtttc ccttttaatg tttgaatggt agcgagacgg
     5881 gcaaacaatg aacgtgattc aggtgaaaat agatccgtaa ggtcagaggg tgcataataa
     5941 gcaactactc cgcatattcc tttcattctg cctttatcac gcctatttga ataatgtgat
     6001 gaatatagca aagacaagtg tcctcctgca gacaacccca ttaaaataat tttatgcgga
     6061 catatgcgta attctttagc atgggatttc aggtagctta gtgcattgtc ataatctgat
     6121 agaatgtctt ccatatcatt ttttattcca aatcgataat caattgaagc agcaacaaat
     6181 ccgtttgcag caagatacct gcaccatgaa atattatttg gctgattgcg gaatccagat
     6241 acccatcccc caccatgtgc aaatagtacc agcgggtaat ctcctttacc tttctcctga
     6301 ttaggatacc agatatccat ttttaattca ctatcttcta tggttttgta tacttttgta
     6361 atatactgga attcgccttt gttttcaatt ggaagacggg cggtaaagat attccacaga
     6421 taaatgacaa tatagcttaa gaaactataa aaagcaagtg accaatcagt tacagccagt
     6481 aacagaaaaa gtacccacag atatagtttg tttacaataa aaactgagtt ttttaagctt
     6541 tcaattttct tttgtatatt cttctgcagc atgtattagc tcccgtccca tacctatata
     6601 tccatattga caacacacct gtctgctaca aatacgatta cccatttttt aagttttgat
     6661 aaactttgaa gctcactgga actgtgatac tttctaattt gttttgttgc ttcgtttttc
     6721 tttccctcta ctattttatc gccatatatt tcatattcca tttttttaat atatttaacc
     6781 tcaaatatgg cgaaatagga tggttctatc ggctcccttc ttagtaatgc tatatctata
     6841 tatccatctt ccacctcata ttctgattta accatataaa tattacttaa cattaaatat
     6901 gaaagcataa taagctttat atatttttca tcgaaattaa tataatcccg attggaaagt
     6961 ttgtgcaatg tcttttcgat tactgatata aatttatcat ttttgccttc aatagcaatt
     7021 tgccttacac cttccttgat aaaggatgta tttatatcat agcctgtttc ttcattaatt
     7081 tttttagcgt aatactggta gtataagccc ttaataatat agttaggtgt ttttaaactt
     7141 acactatcca atactctttc atctattgta agaaggccaa gataaaataa cagtgattta
     7201 aagtcatcag ttgtaaaatc tttttccatg ctgaattgcc tggttatctg cgctactatt
     7261 tcctcatcat ttagtatttt ttccagaact tccatattac ctttccggtt ttttaactca
     7321 aacattttac ccaatttacc atagtcactg gcaatgtttt ggtctattag ttctataggt
     7381 ccttctttat cttctatata tgttttcaaa taatatagaa tcatgtcgct attaaatatt
     7441 cttttatgtg ctttttccga aaacaaatac ccattataat ttttgtggag tacctccatc
     7501 aatgtatcca tttcatttct gccaattgga aattttactg tttctttaat catatacctt
     7561 acttcatcct cagtaaaacc catcatctca ttaaaggctt cttcacgggt tatgttatct
     7621 gcaatattaa atccactggt aaggctatct aaagtaatcg ggcttactcc tgttgcaaat
     7681 atacgcttta caattgtttt tgtctgcttt tttagaactt cataccattt tctaacaaat
     7741 ccagtattac tcactgattc cttaaagaaa tcaaactgga aacttagaag ttcgtttgca
     7801 aaatgatcat actcatctat aataacataa attggttttt caattcttgt agtaactttc
     7861 gtgaaaaaat cctcaaaaat attagctgca gatcctgttt gcaaataatc aaaatgaatg
     7921 ttatgtctgt tttggaataa ttcaaaagca ttctttgtta tattgataaa actctcttct
     7981 aattgttctt ttgtatcagt agtaacccct gtaaaatcaa aattaaggac ataatagcta
     8041 tttctttctt tggttggatt ttgtcctata tatgtattcc taaataaagt atcaaaatta
     8101 tccttttcgt ttatgtcata ataattctgg agcaaagata caaacaggct tttgccgaat
     8161 cttctcgggc gcaggaaaaa taaataatac gaatgtaatt tttccagctc ttctatatac
     8221 attgttttgt caacatagta taaattttcc tctaccatca tcttataatt gctgatacca
     8281 tatggtagtt ttttcaagct aatccactcc ttttaaacac ttactggttt atcttcatca
     8341 tccttatctc tataagccta accagttcct cagcccggtt tgtgatataa tccagatttt
     8401 ttccttctat catgaccctt accaggggtt cagtacctga tggccgtatt aataccctgc
     8461 cttcgccatg taattcctct tccaggtgcc tgcacatatc cgcaattacc tgatcttcaa
     8521 gatacttata tttattatcg ttactcactc ttgcatttcg gagcacttga ggaaatactt
     8581 ccattactcc ggcaagctct gaaagctttt taccggtaac cttcataact cttagaagct
     8641 gaagagcagt tataaggcca tcccctgttg tgttgtaatc cagaaaaata acgtgaccgg
     8701 actgctcgcc ccccagaata tatccattgt taagcatctc ctccaggaca taccggtctc
     8761 ctactttagt ttttactata ttaatgcctt ccctggcagc cataacatca aaacctatat
     8821 tggtcattac cgttacaaca atagtgttct ttgcaagacg tccttctttt ttcatctgca
     8881 gtcctgttat ggccaatatt ttatctccat caacctcatt tcctatttca tcacatgcaa
     8941 gaagtctatc ggcatcgccg tcaaaagcaa gcccaatatc agcaccgcat tttctggtga
     9001 agctacagat attctcaata tatgtagagc cgcatccatt atttatattt ataccattag
     9061 ggttctttgc agtaataaaa atttcagctc caagcctttt caatatttcg ggtgcaactc
     9121 tataagacgc tccattggcg caatccactg caatccttat acctttaaga tccacatcaa
     9181 ttgaatcgtc tataaaagcc aggtaatctt cagcagcact accgacaatg ttttctctgc
     9241 caatgcctct ccctgctatt tcaggcaatg cttcccttcc atcaagaata atgccctcta
     9301 tactatcttc tatatgatca ggcagcttat aaccccttcc atcaaaaaac tttattccat
     9361 taaactcaaa tggattgtgg gaggcagata taacaattcc tgcgtctgcg ccataatacc
     9421 ttgtaagata agctattgca ggtgtaggta taacaccaag aacaacagct tctgcaccca
     9481 ttgaacaaat acctgatact atagctgatt ttagcatatg cccggatatt cttgtatcca
     9541 tacctacaag tattttaggt acatgttttg tttcttgcgt aagtacataa gcaccggcct
     9601 tacccagttt atatgcaagt tccggtgtca attccacatt agctatacct cttactccat
     9661 cagtaccaaa aagccttccc attatttttc cccctaaaaa aaatacatag tgacaagtat
     9721 atatattaat gacctgtatt gtatatataa cttgctttcg ctattaatga cttagctggt
     9781 tatactctcc gtattgttac tactatcctt accatctgtc cttccatctt cattggtgtt
     9841 ttcttcagga ggcttttcat tcttgacctt agttatcttc acttcaacag aaggcaattt
     9901 tataacatct atatccggac ttggtgaaac cttcaactgc acatgatgta caccttcctg
     9961 cagcccttct acatcaatat ggggcgtaag gatacttggg tttatgttgt ttaaactggc
    10021 agttcttcct tttaatatta ccttgcaact atctgtcaat atttcatatt taagttgact
    10081 gccaccatta tcggagttat ttcccatgtt tcccatatta gcagcgacgt tttctatatt
    10141 aatactcatt ttgctaataa caatttctct ctcttccagt ttttctactt ctattgcaac
    10201 agttgcatca gcttctgtat tataaagctt tacaccctca gataatttta taggcacttt
    10261 gtaatccttt gcagctgtta acccatcaac attaatagtt tctgtgctca aagacgttat
    10321 ttttgaaatt atatcataag aacctgttat aacagcatta ggtgcattca cctttattcc
    10381 cgatattaca tggtcagctg caggttcacc tgtaataacc ggttctatgg gtacctcctt
    10441 ccccacttca agaactatat ctacagtaaa ctgattacta agggatgaaa tttcttctcc
    10501 ttttgtatta tatacggtgc atgactgatt gatgactttt ttatccctgt caataccatt
    10561 tatatcaact acagctttta ctgaggatac agtatttata agggactctc tatcatgcag
    10621 agatacatat tcaggcttag ttgcagtttt tataaccata taggattgct ttggagttcc
    10681 cttcagttca atcattacag gcaattcctt tttaagtaca tgctcaagtt caatgggaat
    10741 ctctttagga gacatgccgg ttatagttat ctgcttgtca ttctttgtat aatatgggtc
    10801 atctactttt attgatttgt catttaccga tcgtactttt gagaaatcca gtacagcttc
    10861 aaaatcagct gagcttaaac tatccaaggt actttgtctt ccctggacaa ctacctcaac
    10921 tgttctccta taatttttgt ttaccaggta tatgtttttc tcctgaagtg tattttcatt
    10981 tctcacatcc aattcaatat aaaacttctt ttctatatat ggattgttta tattcaatac
    11041 aataaaccat aatataatac cgaggaatac agatattaac tttgtggcaa ggtcttcact
    11101 taacttgtta tttaacctgt taattatgta cctcatttat tcttcacctt ccataacgtt
    11161 aactttctgt tggatggact attttctaaa agattcttat ttagcgcttt tctcaaagta
    11221 tcaggagtaa gatttctggt aagtccacca cctaatgcaa aggatatctt ccccgattct
    11281 tcagatacta caactgctat ggcatcagat acctccgtta tccccagtgc cgccctatgc
    11341 cttgttccca gttccttact caaattgggg ttatcggtga gcggtaaaaa gcaagatgca
    11401 gcctttaatt tgttatctct tattacgaca gcaccatcat gcaacggagt attaggagta
    11461 aaaatattaa taatcagctc tgacgaaata tttgaattga gttcaatacc tgttttaata
    11521 atctccccga tcttggtatc tctttcaaca actataaggg cacctataaa attccttgat
    11581 aactcaatgc atgcctttac aatatcttca atcattgctg tagttcttag cttgtcttcg
    11641 ctgtcttcaa agttgaaaaa atccctgaat ctgctccttc ctatttgttc cagtccacgc
    11701 cgcagttcgg gctgaaacaa aacaaccatc gcaagagcaa gtatggatac cgtattactc
    11761 aaaatatatg ctattgtatt aagttttaac agtttgctca actcagcggc tacgagtatg
    11821 aaaataatac ccttgatcag ctgccaagct cttgtttccc taacaagaat aataatcttg
    11881 taaattacat atgttacaag actaatatca actatagcac gtatgaccga taaaggtccc
    11941 gtaattccta cataccctac aaagttgtta aacaactcct taatatttat tttgtagaaa
    12001 aaatccaagc aacctcacac ctttatgaat actcttaaaa agtatcgttg tgttataaat
    12061 ttatcatttg aattatacct tgttttttca ataaatgtaa atacatttta tattttttaa
    12121 tataaatgaa atacattcac tacccactac ccacttatac attacccaga taaacatacc
    12181 ttaaataatg agaagctttt tccgctgctg cattaagagt ttttagcggc gttgctggct
    12241 tgttcatcct gtaattggga aagtatctgg ataaatgcaa tactatgtct tttgatattg
    12301 atgcaagcca cttggccaac tctcctattt catccatgga atcatttaat tccggtatta
    12361 ccagagtagt tacttccaaa tggcactttt gggaagcctg ctcaatagta ctctttatat
    12421 attccaaagc tccatctccc ttgcacacat ccttgtaaaa agaggaatta aaagccttta
    12481 catctatatt cattgcatct atatatggca tcagattctc taatggctca ctatttataa
    12541 atccatttgt taccagaaca tttaaaaggc cttttccttt tatgagctta gatgtttcgt
    12601 acacaaattc ataccatatc gacggttcat tataggtata tgctatacca atattacctt
    12661 cagaaacatt tttttcggtt tcctgcacca accgtgacgg agtaatttcg aaggattccg
    12721 gaatgtcatg agctatagtc cagttctggc aaaatgaaca ctttaaattg caaccgaagg
    12781 tg
//
LOCUS       DAOSDD010000122        12670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1910_length_12670_cov_4.951249, whole genome shotgun sequence.
ACCESSION   DAOSDD010000122 DAOSDD010000000
VERSION     DAOSDD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_12670_cov_4.951249"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..769)
                     /locus_tag="PK733_11405"
     CDS             complement(173..769)
                     /locus_tag="PK733_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ11186.1"
                     /translation="MQLQHTQLKAGRQKILFFNKCRNCFIRYIYYNETRLITMFTFAE
                     GVPIKTTLEKNLAMRFGEIIADLHKKFNEFKSEHQRYKIDIDYLISEPIKMLQVYSNK
                     HGLENLNFFEPYIKYLYSQLRELPVNNEAYGIIHDDLNPSNIHIDNSGNITVFDFDHC
                     AYGWRIHDLAVIKLCYDKNTYEAILKGYINRRKLDKKK"
     gene            complement(790..1002)
                     /locus_tag="PK733_11410"
     CDS             complement(790..1002)
                     /locus_tag="PK733_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOJ11187.1"
                     /translation="MNYSAGKYLNICLGYEYEKGLELIKNVTGNSPLLFKAYPDESRI
                     KIIELVKKGELCATDISESLDIPMST"
     gene            1325..1780
                     /locus_tag="PK733_11415"
     CDS             1325..1780
                     /locus_tag="PK733_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOJ11188.1"
                     /translation="MVHCGHDIGTEELAHLEIVGTMVRQLIEGASIDEIKAADLGSYY
                     ADHDKAVYPVSAAGVPFSAAYIQSKGDPVTDLHENMAAEEKARSTYEWLINLSDDPDV
                     IDPLRFLREREVVHFQRFGESLRIVQEYLDSQKYFTVVKTPDMACPTKK"
     gene            complement(1843..2031)
                     /locus_tag="PK733_11420"
     CDS             complement(1843..2031)
                     /locus_tag="PK733_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11189.1"
                     /translation="MYKKNKNLDVEATDSRNDDINSVTKGYLYTKVFCPRCGGVNIYR
                     HRIFSRKYLCNDCRNKWQ"
     gene            complement(2267..3727)
                     /locus_tag="PK733_11425"
     CDS             complement(2267..3727)
                     /locus_tag="PK733_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11190.1"
                     /translation="MKKNWIGYLLVFILLIPNIVHATPLDFSGGVHNEYVYEEMVFIT
                     GAPVKFTGTYSVSENERKGKITVNYKFSLKPEDKSIDGKLDRNITFITTLTNHGDKSQ
                     TVGQTEISKYSETIKIDGNTYTLTDYQFSKSDNIDNMPASDFYLGIIKGRKHYNINRT
                     EGEVIIDISGGNAGYKNFWGSTETLIIDQVITSKYYISDAANEGTQSWQGTISIQVSD
                     SLTKTLKYSENSVNLSSFEGGFARVTNRSVVSRYEYNLPDDKGTIKLNQSMVPKLEML
                     IVPKFRDVNGHWAQDEIKKLYSLEVFEDNSEIFAPDITVNRLEFIKAVIRACDIRPSL
                     DEGKTAVKSKNEPLEVSKFIDISVKHPDYKYIKEGLNKGIITGTSGNTFGPAEPLTRA
                     QAITILIRALGFENKAPNPGYVTSFADDSKIPNWAKDSIYVAREVNLVSGDTYNRINP
                     DKVLTRAEASSMLVRFLEFLEKDLQKDYRENIMNFY"
     gene            complement(3797..5491)
                     /locus_tag="PK733_11430"
     CDS             complement(3797..5491)
                     /locus_tag="PK733_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11191.1"
                     /translation="MSRMDGIKRINRMNRKISIALTALIFFSLFMSVVHADKTTPYIT
                     GIIEGYFIKYEEGKVWVEEYDGTLHSIPLIRNPSLLIDGRNVSITDFKYGMEIYGDIK
                     DKMISNMNAFSVDLPGYILPGGKVRTGLVREIRRDKIKIKLPSGKEETYLISPATVTL
                     KNGEYMSLSTLYEGDMVKLYFDDIKTTLISRIEIEGNSIIIKDIYKATLIIADEWEDA
                     IVLGNVEVFRNGKWESVSNSLKLPYNTDFPLYVGSVKVPYKNMKYYKGKTVYMIIKDF
                     FGKDRIERIIVKNQYESAFSDKIEKINWYASQIELANYKNISFYEGTIVIKSGRLSDI
                     YALTPKTDGLIIADSSGDFATADIICVYNEDINNSNIGQDCIYAGRLDTILEDKLYIK
                     DFSLLSKNDWAYHGYEKELYYYSDTFIYDLENKKQISSKEFLAGNYAVDEDNLQYRNL
                     KDWYGYIYTDEDRITSIYVKKTMDYLSQQRITAGIIETRPEHNQLMGWTVKLADAKDW
                     SSRHRLWMPKNASLNLYIRETMIMKNSVRVEPEDLKEGDRLYIVRDDANCKVVIVK"
     gene            complement(5469..5609)
                     /locus_tag="PK733_11435"
     CDS             complement(5469..5609)
                     /locus_tag="PK733_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11192.1"
                     /translation="MERNMGTVLLFQTHEVTKGQSFHIISGEQTKDSGGMVVNYEQNG
                     WN"
     gene            complement(5618..11836)
                     /locus_tag="PK733_11440"
     CDS             complement(5618..11836)
                     /locus_tag="PK733_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOJ11193.1"
                     /translation="MKRYLSFVLALMIILGGLPVCNTVVYAADGEPVFTGITVTKVFR
                     DTYGAEKYSILINGQNFIKDGNNLVEVGIMSDDGTIRSFSPVYKSPVLVQYELKPNEI
                     SNQVYINGYRLDISETDIPNITNKTPTAGIVDGNGEAEVTLVGSKFHNITSRKVEASL
                     FQGNTDLPITEVEADNKIKSAALKGKTGIWSVQFKKTSSVNIGNSTNVMFIIQHRYLD
                     IFTVLNKLNVSDKVELIPTQGTAESIVTLKGDSLKPVNEMSVFFLKSLDNKYASANLG
                     TGENYRRDEKEQRDIFAVKVPKGLSVGSYYVVLTNNVDPAKDIKDQIKSYKVLDTKFT
                     VIDRKNSVSLDSITPGKGPAKGIDAVVKGINIGRISSAIYESGTRSEPDITTNSSKMS
                     IEYTGGKYKLIGENGVQVKKLTREITIHVGGPVRFREGSDFNKDTFDYINIRVEENND
                     NQKLVKEIIVEITTRITYIENGVEKIAEIVETDTLKNGFTYEPLDYIPKITSIVPDRI
                     PVNNENKVLPGLKLSIIGENFLLYRYMDKDGNIRYKYPKIDLGGQRVLDPNNEKIEIK
                     VFDKSGREIDGTANNDIGSKILITIPRDVLVKEDIINNPVDLRVTNPIRSPDSNDEGS
                     SATGKIIFARPAYDRTPVITSIIPNTVTIDGEKGIKIIGQNFGQNASLFINGVKISGI
                     KRNGTGTEITFDAPPNNEGYVQIVVQNEEGGIAIYNDFLYVKTYTSPKFIDFNPKKGT
                     ANTLVNVKGENFVPPNPLVKDLEGIGIIKLIGTRIMLGGEDVNSYNLGKDGRTIELQP
                     YNTPAANPLFQNNGGLLKLADYYYSVILEDEKTTAYYKIYFDAKTGKTLLTDGYKEVY
                     ELYVKDNKENKFYGKKVGETETELKINSEPDTGSTESIEIDSIILNIRTPYEIEKVNG
                     IDTITGNRVKVINSNELIFKVPSKQREGYYDLAIVNPDTKKDEKKGEQGFYYSFHPND
                     PPPFIEKIEPDEGSVDGQYYINIIGRNFIDRGSNDQKSQVIIGGIAVDPKDVEGSPDG
                     KILTVKAPKYPGDLAKETDMDRKTVPVIVVNPDGGSDSKEDGFTYVVPISHPKITKLI
                     LDRGSAAGGELVTIEGSDFRFFEPYRDLNNNSQWDYGEPFTDLNNNEKWDDLRYWLSP
                     KLKEEYDSLAENLITPILPKIYFNGKPAKVKGFTAATIEVETPKGVKGPAEVYLVNND
                     YGVSNKVTFTYEATNPKIRKIVPNTGKKQGGDKIEILGEYFFESRIKVYTSEKKIADK
                     NMPLIQFGSKNDANISNKEVPIELPNSGRIKDKFAEVKVGNLTVSYSAAEDIRKLSFI
                     IEENKEKFMLNDVNYDDTEAFLPVNLLKNSKDESYNGYEYVRIRLERITGAVSTNRLR
                     IDRGFSPEAVLQNIGQILIRTPSYYTVGATPVIVSNPDGGEASGEFIYKNPNSKPVIT
                     NILKDGEQGKLEDGKKIIKVNYKGGNLVDVLGLDFRRPVTIKIGDVVEIKQGIEYSPA
                     NEAISNKLTFKMPAVAEKYVNNVYRLVAENEDGGVAGSDEAATPIYIQIIKGESDGLA
                     ITKVTPNTGPAAGGTLVTIEGKDFRSKMDGFPDGKFSVYFGDGKNQVRIRNENIVSIA
                     TNKIQLRTPAYSYGTVPIKVENPDGNMAELANAFTYISNPEISYIADPENGDIVINVI
                     SVEGGRKIKIVGNDFMPGARIIFNPVIRNIRNEQNATGEVIIIDTEKYVLESGNNGTE
                     VEVINGQVITVVVPPGKIDTKGVMVINPDKGASPIYNIAYGIPEIGAPQNVRAEIAYD
                     QYIKVSWKGVTGAEEYELYVSEEGKNFEYIGSTELLNYVYKKIKPTTTYKFMVRAFGK
                     YGSSKPLYASRSNLVKTGPGVVPNNNDGELAEKTTIQRMGNTVNVVIGFGDYRNLYDA
                     DLVIDITKGSLAGSAEVIVSIPASIVVNDRNKSIAIVGKDYKVKFKPSVFKSLSMTEN
                     ENRDDAGVKFKISTYKGSIELNNNDMTVLSNKYILEAYTFAGADSTAVDHLNDNMELD
                     LDYDGRKSIARGMKRIYTARYEGRSNVWEKLPYSSPYAGSTEIIDRMGIYAIIGRK"
     gene            12173..12667
                     /locus_tag="PK733_11445"
     CDS             12173..12667
                     /locus_tag="PK733_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ11194.1"
                     /translation="MIEKTSCKKYRDADQKNYDKVMNIIDLYKDKEGCLIQVLHEAQK
                     IYGHIPLELQQIIADGLGLPLSEVAGVVSFYSFFSVQPRGQHTIRVCMGTACYVRGGK
                     KLVEHLLKTLNVELGGTTSDGKFTFEVARCIGACGLAPAIMIDDVVYKQMTPAKVNIL
                     LSEF"
BASE COUNT         3591 a         2610 c         1810 g         4659 t
ORIGIN      
        1 attttgaaag gttttgtcgc attttattcc gaagaatttt tactcctcag agctgaaata
       61 tgtataatat acatgtgttg aaatctataa tataggtttt gcctagttgc acattatatt
      121 tctgaaatgt agcaataaga gaagaatact atccaaagca tataaagact ctttacttct
      181 ttttatcaag tttccttcta ttgatatatc cttttaatat tgcttcatac gtatttttat
      241 cataacaaag ctttataaca gccaaatcat gtattctcca accatatgca caatgatcaa
      301 aatcaaatac tgtgatattt ccactattat ctatatgtat attggatggg tttaaatcat
      361 catgaattat gccgtatgct tcattattga caggtagttc tcttagttgg ctatataagt
      421 attttatata aggctcgaaa aagttaagat tttcaagacc atgtttattg gaatatactt
      481 gcaacatttt aataggttca cttataagat aatcaatgtc tattttatac ctttgatgct
      541 cgcttttaaa ttcattaaat ttcttatgta aatctgcaat aatttcgcca aatctcatag
      601 ctaaattttt ctctaatgta gttttaatag gtactccttc tgcaaatgta aacattgtta
      661 ttaaccttgt ttcattatag tatatatagc ggataaaaca gtttctacat ttattgaaaa
      721 ataagatctt ttgccttcct gccttcagtt gagtatgctg gagctgcaaa gaatgtctaa
      781 atgatgtgat taagttgaca ttggaatatc caagctttca gaaatatcag ttgcacaaag
      841 ctctcccttt tttactaatt caataatttt tattctgctt tcatcaggat aagctttaaa
      901 taacaatggg ctattaccgg ttacattttt aataagctcc aatccttttt catactcata
      961 tccaaggcat atatttaagt atttgcctgc actataattc atttattgct atttcctgta
     1021 cacgctccag ctttttatcc tgccgttgat cttcggtagt aaacaactca tcaatcggca
     1081 gtaaatttat ctcagaatcc tttttcactt atcatcaccc cctttcccgc agggtatggc
     1141 aagcaggata tccacttcat attgaggctt gtccgatata ataaaatcac attgattttg
     1201 cgggctgtag cagctgcact tctcaaaaat actgatatgc tttaggaaat tcagattttg
     1261 cacattcgcc ttttggcact tttccgagcc gtaaaccccg cataaatcaa gacttttatc
     1321 gcctgtggta cattgcggac acgatatagg aaccgaagaa cttgctcacc ttgaaattgt
     1381 aggtactatg gtaaggcagc tgattgaagg tgcaagtata gatgaaataa aggctgctga
     1441 tcttggatct tattatgctg atcatgataa ggctgtttat ccggtaagtg cagcaggtgt
     1501 gccattttct gctgcttata ttcagtccaa gggcgaccct gttacggatc tgcatgaaaa
     1561 tatggccgct gaggaaaagg caagatccac atatgaatgg cttattaact tatccgatga
     1621 ccctgatgtt atagatcctt taagatttct aagagaaaga gaagttgtcc attttcagag
     1681 atttggagaa tcactgcgca ttgtacaaga atatttggac agccagaaat atttcactgt
     1741 tgttaagact ccggatatgg catgcccaac aaaaaagtaa tactaaataa tgaattttaa
     1801 ccacccgtct tccagatagc gggtggtttg ctcctatctc gtttactgcc atttatttct
     1861 gcaatcgttg cataaatatt tacgagaaaa tatacgatgc ctgtaaatat taaccccccc
     1921 acatctaggg caaaaaactt tagtataaag atacccttta gtaactgagt tgatatcatc
     1981 gtttctagag tctgttgctt caacatcaag atttttgttt tttttataca taacacattt
     2041 cctcccccaa aaaagctttc tcccctaaaa aaagcattat tacaagtatc tatgttatgg
     2101 ttttttaaac ttttgtaatc agtttatgcg gatttcatta aagtaaaatt gcaatcatac
     2161 cccgcttcct tatattaatc cttataacct atgttagcat actcgccatc atattatcac
     2221 atttaccaaa gattgtatac ccgtattttt catcgctaat gtttttttaa taaaaattca
     2281 taatattctc tctgtaatcc ttctgcaaat ccttttctaa aaattcgaga aaccttacaa
     2341 gcattgaaga agcctctgct ctggtcagaa ctttgtccgg attgattcta ttatatgtat
     2401 ctcctgacac aagatttact tctctagcta catagatgct gtcctttgcc cagttgggta
     2461 ttttgctgtc gtctgcaaag gatgtcacat agcccgggtt tggtgccttg ttctcaaaac
     2521 ctaaagctct aataagtatt gtaatagcct gtgctctggt caatggttct gcaggtccaa
     2581 aggtattccc tgatgtacct gttattattc ctttatttaa tccctctttg atatatttgt
     2641 aatcaggatg ttttacagat atatctataa attttgatac ttccagaggc tcatttttgc
     2701 tttttactgc ggttttccct tcatccaggg aaggccttat atcacaagct cttattaccg
     2761 ctttaataaa ctccagcctg tttactgtaa tatcaggtgc aaatatttca ctgttatcct
     2821 cgaatacttc caatgaatac agcttcttta tctcatcctg tgcccagtgg ccattaacat
     2881 ctctaaattt tggcacaata agcatttcca actttggaac catactttgg tttaatttga
     2941 tggtaccctt atcatcaggc agattatatt cataccttga tactacactg cgattggtaa
     3001 cacgagcaaa tccaccttca aaacttgaaa ggttcacact gttttcagaa tattttaatg
     3061 tttttgtgag gctgtcagat acttgaatgc ttatagtacc ctgccatgat tgtgttccct
     3121 catttgctgc atcacttata taatattttg atgttataac ctgatctata atcaaagttt
     3181 cagtacttcc ccagaagttt ttataacctg cgtttcctcc actaatatct attataactt
     3241 ctccttcggt tctatttata ttgtaatgct ttcttccctt tatgatccct aaatagaaat
     3301 ccgaagcagg catgttgtcg atattgtcag attttgaaaa ttgataatca gttaatgtgt
     3361 atgtattccc atcaatcttt atggtttcac tatatttgct tatttctgtt tgtccaacag
     3421 tttgactttt atccccatgg ttggttaaag ttgttataaa cgtaatattt ctatccaatt
     3481 tgccatctat ggatttatct tcaggtttta atgaaaattt ataattaaca gttattttac
     3541 ctttcctttc attttctgaa acactgtaag taccggtaaa tttaacaggt gcccctgtta
     3601 taaataccat ttcttcatat acatattcat tgtgaacacc accagaaaaa tccagtggtg
     3661 tagcatgcac aatatttgga attaacaata taaaaactaa taagtaacct atccaatttt
     3721 tcttcataaa aacctccata tctttaatgt gccaaatgaa ttgtcacatt atctcttgtg
     3781 cacaaaaagt tctatattat tttacaataa caactttaca gtttgcatca tctcttacaa
     3841 tatacaacct gtctccctct ttcaaatctt caggctctac tcttacacta tttttcataa
     3901 tcatagtctc tctaatgtat aaattaagag atgcattttt gggcatccat aacctatgtc
     3961 tgctgctcca atctttagca tctgcaagtt ttacagtcca gcccatcaat tgattatgtt
     4021 caggcctggt ttcaattatt cccgcagtaa tcctctgctg tgaaaggtaa tccatggttt
     4081 ttttaacata tatgcttgta attctatctt catcagtata aatatatcca taccaatcct
     4141 tgagatttct atattgcaga ttgtcttcat ccacggcata atttcccgca agaaattcct
     4201 tactagatat ctgttttttg ttttccaggt cgtatatgaa ggtatcacta tagtaatata
     4261 attccttctc atagccatgg tatgcccaat cgtttttgct aagcaaagaa aagtccttta
     4321 tataaagctt atcctctaaa atagtatcca gtcttcctgc ataaatgcaa tcctgcccaa
     4381 tatttgagtt attaatatct tcattgtaaa cacagattat atcggctgtt gcaaagtctc
     4441 cactgctatc agctattata agtccatcag tcttaggagt aagagcatat atatctgaca
     4501 gtcttccact ttttatgact attgttcctt cataaaaaga aatattctta tagtttgcca
     4561 attcaatctg agaggcatac cagtttattt tttcaatttt atctgaaaat gcagattcat
     4621 actggttctt tactatgatt ctttctattc tgtccttgcc aaagaaatcc tttataatca
     4681 tatatacggt cttgccttta taatatttca tgtttttata tggtactttt acgcttccaa
     4741 catatagagg aaaatccgta ttataaggca gctttaatga attgcttaca ctctcccact
     4801 ttccatttct gaatacttca acatttccca atacaatagc atcttcccat tcatcagcta
     4861 taataagagt tgctttgtag atatccttaa ttataatgga atttccttct atttcaattc
     4921 tgcttataag ggtggtttta atatcatcaa agtaaagttt aaccatgtct ccttcatata
     4981 aagtgctcaa ggacatgtat tccccatttt taagtgtaac agtagcaggt gaaattaaat
     5041 aggtttcctc ttttcctgat ggaagcttta tttttatttt gtctcttcta atctccctta
     5101 ccaaacctgt tctaacctta ccgccaggaa gtatgtatcc aggtaaatca acagagaagg
     5161 cattcatatt gctaatcatt ttatctttta tgtctccata tatttccatt ccgtatttaa
     5221 aatcagtaat ggaaacattt ctcccgtcaa ttagaagaga tggattcctt attaatggta
     5281 tagagtgcag agtaccatca tactcctcta cccatacctt accttcctca tatttaataa
     5341 aatacccttc aatgatccct gttatataag gggtagtttt gtcggcatgt actacagaca
     5401 taaataagct aaagaaaatt aaagctgtca gtgcaattga aattttcctg ttcattctgt
     5461 taattctttt aattccatcc attctgctca tagttaacca ccattccccc actgtctttt
     5521 gtttgttcac ctgatatgat atggaaggac tgtccctttg tgacctcatg tgtctgaaat
     5581 agtaaaactg tccccatgtt tctttccaag tttcttttta cttcctccca ataatagcat
     5641 atatccccat cctatcaata atctccgtag agcctgcata aggcgagcta tagggaagct
     5701 tttcccatac gttgctgcgg ccttcgtacc gggctgtata tattcttttc atcccacggg
     5761 ctatggattt ccttccatca taatctaaat ctaattccat attgtcattc aaatggtcta
     5821 cagcagtaga atccgcacct gcaaatgtat aagcttccaa aatatattta ttagacagga
     5881 cagtcatatc attattatta agctctatac ttcctttgta ggttgaaatc ttaaacttca
     5941 ctcctgcatc atctctattc tcattttctg tcattgaaag gcttttaaat acactcggtt
     6001 taaatttgac tttgtaatcc tttccaacta tagctatact tttatttctg tcatttacaa
     6061 ctatagaagc aggaatgctt acaataacct cagcacttcc ggcaagactg ccttttgtaa
     6121 tatctataac caaatctgca tcatataaat tcctgtaatc tccaaaccct ataactacat
     6181 taacagtatt gcccatcctc tggattgtgg ttttttcagc aagttcacca tcattattat
     6241 tgggtactac tcccggacct gtctttacta aattgctcct gctggcatac agtggttttg
     6301 aagaaccata tttgccaaaa gctcttacca tgaatttata tgttgtagtg ggctttattt
     6361 tcttatacac atagtttaaa agctctgttg atcctatata ttcaaagttt ttaccttctt
     6421 cacttacata cagctcatac tcctcagccc ctgtaacacc tttccagctt acctttatat
     6481 attgatcata agctatctct gctcttacat tttgaggcgc tcctatttcc gggattccat
     6541 aggctatatt atatataggt gaagctccct tgtccgggtt tattaccata accccttttg
     6601 tgtcaatttt cccaggagga acaactacag ttataacttg tccatttatt acttctactt
     6661 ctgttccatt attgccggat tccagaacgt atttttcagt atcaataatg atgacttctc
     6721 ctgtagcatt ctgttcgttt ctgatattcc tgattaccgg attaaaaatt atccgagcac
     6781 ccggcataaa atcattaccc actatcttta ttttccttcc gccttcaact gaaattacat
     6841 ttataactat gtcaccgttt tcaggatctg ctatgtaact aatctcggga ttacttatat
     6901 aggtaaaggc attagccagt tccgccatat ttccatcggg gttctccacc tttatgggta
     6961 cagtcccata gctgtaggca ggagttctaa gttgtatttt attagtagca atagatacaa
     7021 tattctcatt tctaattctt acctgatttt tcccatcgcc gaaataaaca ctgaactttc
     7081 catccgggaa accatccatc ttgcttctaa aatcttttcc ttctatagtg actaaagttc
     7141 ctccggcagc aggtccagta tttggagtta ctttagttat agccaggcca tcactttctc
     7201 cttttattat ctggatataa attggtgtgg cagcttcatc agaaccagcc actcctccgt
     7261 cttcattttc cgctaccagc cggtatacat tgtttacata cttttcagct acagccggca
     7321 ttttaaaggt tagtttattt gaaattgcct cattggctgg agaatattca atgccctgct
     7381 ttatctcgac tacatcccct attttaatag ttacaggccg tctaaagtct aaacctaaaa
     7441 catctacaag atttccacct ttatagttaa ctttaattat tttcttacca tcttctaatt
     7501 tgccttgttc tccatctttt aaaatatttg taattacagg tttactattg gggtttttgt
     7561 agataaattc ccccgaagcc tctccgccat caggatttga gacaataaca ggtgttgctc
     7621 ccacagtata atatgatgga gttctaataa gtatctgtcc aatattttgc aaaacagctt
     7681 ctggagaaaa tcctctatct atacggagtc tgttggttga aacagcccct gttatccttt
     7741 caagcctaat tctgacatat tcataaccat tataagattc atctttactg ttttttaaaa
     7801 gatttactgg taaaaatgct tctgtatcgt cataattcac atcatttaac ataaactttt
     7861 ccttgttttc ttcaataatg aagcttaatt ttctaatgtc ctctgcagca ctataggata
     7921 cagtcaagtt gccaaccttt acttcagcga acttgtcttt tatcctgcct gaattaggca
     7981 gttctattgg cacttccttg ttggaaatat ttgcatcatt tttgcttcca aattgaatca
     8041 gcggcatgtt tttatctgca attttctttt cagatgtata aacctttatt ctactttcaa
     8101 aaaaatattc cccaagaatt tctatcttat cccctccttg tttcttacct gtattaggca
     8161 caatttttct tatctttgga ttagtagctt cataagtgaa tgtaaccttg tttgaaactc
     8221 cgtagtcatt attcaccaga tacacttccg caggtccttt tactcctttt ggagtttcca
     8281 cttcaattgt agcggctgta aagcctttta ccttggctgg tttaccgtta aaatatatct
     8341 ttgggagaat aggtgtaata agattttcag ctaaactatc atactcttcc tttaattttg
     8401 gacttaacca atatcttaga tcatcccatt tctcattatt atttaaatct gtaaaaggtt
     8461 caccataatc ccattgacta ttgttgttta aatccctata tggctcaaag aatctaaaat
     8521 cagagccttc aatagttact aactcaccac ctgctgcgct tcctctgtct aaaatcagct
     8581 tggttatctt tggatggctg ataggaacta cataggtaaa tccatcttct ttactatcgc
     8641 ttcctccatc aggattcact actataactg gtacggtttt tctgtccata tccgtttcct
     8701 ttgctaaatc ccccggatat tttggcgctt taaccgttaa tatttttccg tcaggcgatc
     8761 cttctacatc tttgggatca actgctatac ccccgataat cacctgggat ttctggtcat
     8821 tactacctct gtctataaaa ttcctaccta taatattaat ataatattga ccatccacgg
     8881 aaccttcatc tggctctatc ttttcaataa aaggtggcgg gtcattagga tggaatgaat
     8941 aataaaaccc ctgttcacct tttttttcat cctttttagt atccggattt actatggcca
     9001 gatcataata accttccctt tgtttagacg gcactttaaa tattagttca ttgctgttaa
     9061 ttactttcac tctgtttcct gttattgtat caattccatt taccttctct atctcatatg
     9121 gggttcttat atttaatatt atagaatcta tttctataga ttctgtagaa ccggtatcag
     9181 gttctgaatt gattttaagt tctgtttcag tttcccccac ttttttacca taaaacttat
     9241 tctctttgtt atcctttaca tatagctcat atacctcttt atatccatct gtaagcagtg
     9301 ttttgccggt tttggcatca aaataaattt tgtaataagc agtagttttt tcatcctcca
     9361 agataacgct ataataataa tccgctaatt ttagcagtcc accattattt tgaaataaag
     9421 ggttggcagc gggtgtatta taaggctgca gttcaatagt cctgccgtct ttccctagat
     9481 tataactatt gacatcttct cctcccagca ttatcctggt ccctataagt tttataatac
     9541 ctattccttc caaatctttt actaaaggat tcggaggtac aaagttctca ccttttacat
     9601 ttaccaacgt attagcagtt cccttttttg ggttaaaatc aataaatttc ggacttgtat
     9661 aggttttgac gtaaagaaag tcattgtaga tggcaatacc gccctcttca ttttgaacga
     9721 ctatttgcac ataaccttca ttattaggtg gtgcatcaaa agttatctcc gttccggtac
     9781 cattcctttt tatgcctgaa atttttaccc catttataaa taaagatgca ttctgtccaa
     9841 aattttgtcc aattattttt attccttttt ctccatcaat agttacagta ttaggaataa
     9901 tactggtaat tacaggtgtt ctatcataag ctggtctagc aaaaataatt ttccctgttg
     9961 cagaggagcc ttcatcatta gaatccggac ttctaatagg gtttgtaact cttaaatcta
    10021 cgggattatt tattatatcc tctttaacta atacatcacg gggaattgta atcaatattt
    10081 tagaacctat atcattattg gcagttccat ctatttctct gccactttta tcaaatactt
    10141 ttatttctat cttctcatta ttaggatcta gaactctttg tcctcctaaa tcaatttttg
    10201 gatatttata tctgatattt ccgtctttat ccatatatct atataataaa aagttttcac
    10261 ctattataga taattttaat ccgggcaaaa ctttgttttc attatttact ggaattctat
    10321 ccggtactat acttgtgatt ttaggtatat aatcaagagg ttcatatgta aatccatttt
    10381 ttaaagtatc agtttctact atttctgcta ttttttctac tccattttct atgtatgtta
    10441 ttctggttgt tatttctact attatctctt ttactaactt ttggttgtca ttattttctt
    10501 ccactctaat atttatataa tcaaatgtat ccttattaaa gtcggaccct tctctgaacc
    10561 tgacggggcc tcctacatga atagttattt ctcttgtaag tttctttact tgaacgccgt
    10621 tttctcctat taatttatat ttccctcctg tatattctat agacattttg ctgctatttg
    10681 tagtaatatc cggttcactc cgggttccgc tttcataaat agcactgctt atcctaccta
    10741 tattgattcc ttttactact gcatctatac ctttggcagg tcctttgcca ggggttatac
    10801 tatctaaaga aacactattt tttctatcta taacagtaaa ttttgtatcc agcaccttat
    10861 agcttttaat ctggtctttt atatcctttg ccggatcaac attattagtc agaacaacat
    10921 aataggagcc aactgacaaa ccttttggaa cttttacagc aaaaatatcc ctttgctcct
    10981 tttcatctct tctatagttt tcacctgtcc caagatttgc agaagcgtat ttattatcca
    11041 gtgatttcag gaaaaacaca ctcatctcat tcacaggctt taaactgtcc ccttttaaag
    11101 taacaatact ttctgcagta ccctgagtag gtatcaattc aactttatcg gaaacattca
    11161 gcttgttaag cactgtaaaa atatccaaat atctgtgctg aattataaac atgacgtttg
    11221 tactattgcc tatatttaca gaagaagtct tcttaaactg tacagaccat ataccggtct
    11281 ttccctttaa agcggcagac tttattttat tatcagcctc aacctcggtt ataggcaaat
    11341 ctgtattacc ctgaaacaag gatgcttcta cttttctact tgtaatatta tgaaatttgc
    11401 ttccaactag agtgacctca gcttctccat tgccatctac aatcccggcg gtaggagttt
    11461 tgttggttat attgggaata tcagtctcag atatgtccaa ccgataacca ttgatgtaaa
    11521 cctgattgct aatctcattc ggtttcagct catactgaac caaaacaggc gacttatata
    11581 ccggactaaa tgatctaata gtaccatcat cgctcattat gcctacctca acaagattat
    11641 tgccatcttt gataaaattt tgtccattaa tcaaaatact gtatttttca gctccataag
    11701 tatccctgaa aacttttgtt acagtaatac ctgtaaaaac aggttctcca tcagcagcat
    11761 acaccactgt attacatacg ggcaagcctc ccagaataat catcaatgct aaaacaaaac
    11821 ttaaatacct tttcaaacat atcccaccta ttctatcttg taccatttta atataccttg
    11881 tcctatattt ttatatcggc aaaatgctgg ctttctttat aacgcatcag tataaatttt
    11941 tttgacacgt ggggacaacc ctgcattctt actggttttt tattatgaac ttcctgtgaa
    12001 gcagaacact gtgaataaca tattcccatt aaaataatac gatttttcca gtataagaga
    12061 cctaattttt catttatttt tcacattatt agcgtattat taatctatca ggtaattgtg
    12121 gtaatttatt ccttacataa aaattacgat aatacgagta aggagggaat ttatgattga
    12181 aaagacttca tgtaaaaaat accgggatgc agaccaaaaa aactatgata aggtaatgaa
    12241 tatcattgac ctgtataagg ataaggaagg ctgccttata caagttctgc acgaagctca
    12301 aaaaatatac ggtcatattc cattggaatt acagcagatt attgcagacg gacttggact
    12361 tcctctttct gaagtagcag gagtggtatc cttttattcc tttttctcgg ttcaaccccg
    12421 cgggcagcac actatccgcg tctgtatggg cactgcatgc tatgtacgcg gaggtaaaaa
    12481 gcttgtggag catctactaa aaacccttaa tgtggaacta gggggaacca cttcagacgg
    12541 aaaatttaca tttgaggttg cccgctgcat cggcgcctgc ggcttggctc ctgctataat
    12601 gattgatgat gttgtttaca aacaaatgac acctgcaaaa gtaaacattc tattatcgga
    12661 attttaaatt
//
LOCUS       DAOSDD010000123        12634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1921_length_12634_cov_5.143573, whole genome shotgun sequence.
ACCESSION   DAOSDD010000123 DAOSDD010000000
VERSION     DAOSDD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_12634_cov_5.143573"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..160)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(525..1478)
                     /locus_tag="PK733_11450"
     CDS             complement(525..1478)
                     /locus_tag="PK733_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ11195.1"
                     /translation="MILKKMIMIICIFVIPILFIDGIAIIHFANQELEQEMKSRIQEK
                     KNNNSDEYSGNMDNKNRNGNEGEIDEIPVNLLILGLDEDMVRSDVIILVNYKPWESTI
                     NILSISRDTKVHINGADEKINAMIGMGGEKLLISRIEEITGLPVDYYITIGFLAFRKI
                     VDELGGVEIDVPFHMKYDDPVQNLHINLQKGIQVLNGEKAEEFVRYRKGNNPGQGYID
                     GDLGRIEAQQKFIKALIQQKFNLRYFSKTDEIFSILKEHARTNISLNDFRYYLRNINK
                     INITNIKAYTLPGDSVYTDNLWYYIYDKDKTAKLIEEKFSR"
     gene            complement(1681..2919)
                     /locus_tag="PK733_11455"
     CDS             complement(1681..2919)
                     /locus_tag="PK733_11455"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOJ11196.1"
                     /translation="MVRVNENYLKLPGSYLFAEIGRRVARFKDEHPEADIIRLGIGDV
                     TRPLPESVIKSLHEAVDEMAHEKTFKGYGPEQGYKFLIQKVIEYDYKPLGISLDEDEI
                     FISDGSKCDTGNIQEIFGLDNIIAVTDPVYPVYIDTNVMAGRTGSLSETGKFEKVVYI
                     SCNAENNFIPELPKEKVDMIYLCYPNNPTGMTLPKSELKKWVDYARENKAVILYDGAY
                     EAYIQEKGIPHSIYEIEGAKDVAIEFRSFSKNAGFTGTRCAYTVVPKTVMAYTEKGDA
                     VPLNKLWNRRQTTKFNGVSYIIQKGAAAVYTDEGQKQIKSLIDYYMTNAKIIREGLLS
                     LGLQVFGGVNAPYIWLKTPNNMDSWTFFDKLLNEANIVGTPGVGFGPSGQGYFRLTAF
                     GSRESTMEAIERLKVRLKLD"
     gene            complement(2998..3852)
                     /gene="dapF"
                     /locus_tag="PK733_11460"
     CDS             complement(2998..3852)
                     /gene="dapF"
                     /locus_tag="PK733_11460"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOJ11197.1"
                     /translation="MEFTKMHGLGNDYVYVNCVDKCPEKIIENPSAVAKFISDRHFGI
                     GSDGLVLIMPSKAADFRMRMFNSDGSEAEMCGNAIRCVGKYVYDNKLTQKTAVSIETL
                     AGIKFLDMVTEYNQVVLVTVDMGEPVLLPRDIPIKSDKEKFISEPVEIDGRTYKATGV
                     SMGNPHAITYVDDVMNFPLEVIGPKMEKHDLYPRKINAEFVQVIDRSTLKMRVWERGA
                     GETMACGTGASAVLVASVLNNVSERKATIKLLGGDLVIEWKEKDNHVYMTGPATKVFE
                     GRIDISHL"
     assembly_gap    4100..4199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4285..5016)
                     /locus_tag="PK733_11465"
     CDS             complement(4285..5016)
                     /locus_tag="PK733_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOJ11198.1"
                     /translation="MKIRVTVWNEYRHEKRGGKPAEIYPEGIHGAIAAYLKQYDELEV
                     RTATLEEPEHGLTDEVLNNTDVLIWWGHTAHEKVNDEIVNKIYNRVLCGMGLIALHSS
                     HMSKIFTKLMGTSCTLKWREIAEKERLWVVEPGHPIAEGIGEYIELEHEEMYGERFDI
                     PEPDTLVFIGWFQGGEVFRSGCCYNRGYGKVFYFQPGHETFPIYYNQDILRVIKNAVI
                     WARPVKTMAQVSCTQVEPLEEIISK"
     gene            5293..5991
                     /locus_tag="PK733_11470"
     CDS             5293..5991
                     /locus_tag="PK733_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ11199.1"
                     /translation="MDILIIIPAFNEEKSITSVISQIRLHIPEADILVINDGSTDSTG
                     ERAKAAGANVVNLPFNLGIGGAMQTGYLYARYNNYDIAIQVDGDGQHDPSYIHQLIKP
                     VLEDYADMVIGSRYIQETAYKSSAVRRVGMVFFSLLVKFLTDQHVKDTTSGFRAINRN
                     IINYFSLNYPVDYPEVDVLVKLKRKKFKVMEIPVEMKTRKTGKSSITPIRSIYYMIKV
                     TLSLLIETVKTVEY"
     gene            6010..6348
                     /locus_tag="PK733_11475"
     CDS             6010..6348
                     /locus_tag="PK733_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOJ11200.1"
                     /translation="MILNVYSISIAFSVVFLIIVIELVRKNKLQERYSLLWILMSIVL
                     LILSSTPVIINTIADWLEIKNPPSFLFLFGMVYLIIYNLHITTVVSKQSERITKLAQE
                     IALLKEKNEI"
     gene            6454..7371
                     /locus_tag="PK733_11480"
     CDS             6454..7371
                     /locus_tag="PK733_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11201.1"
                     /translation="MGSIKIVKKEYKGWENCVELSNGLVDIIAVTDIGPRIIRFGFTG
                     KENEFCEVADQVGTKGGDEWKIYGGHRLWHSPEHKPRSYEPDNSKINYYEKEDGIVLC
                     QPVEQRALIKKEIEISLSPDEAKVTILHRLTNKGLWPIELAVWALTVMAPGGMEIIPQ
                     ANRETDLLPNRMLSLWPYTKLNDPRVYWGEKYITLQQDSSINTPFKIGMSNEHGWAAY
                     ANHGNLFVKQYNHVLNAVYPDFGASSYETYTCDFMTEMESLSPMVTLEPDEYIEHTEV
                     WTLFHGVKAPQNENEIDNLILPLIPDAKR"
     gene            7484..7897
                     /locus_tag="PK733_11485"
     CDS             7484..7897
                     /locus_tag="PK733_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ11202.1"
                     /translation="MTIKMMTKLKSKDTIPQFLRYITTGGLAFILEYSTFYILSNLLD
                     FWYIWANSIAMTLGFAISFILNRHWSFKSGANPVKQLILYGILFLINLGISNLLMLLF
                     TGTMGIKPLISKIITIGILVCWNFVIYKKIIFKNE"
     gene            complement(8032..8116)
                     /locus_tag="PK733_11490"
     tRNA            complement(8032..8116)
                     /locus_tag="PK733_11490"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8080..8082),aa:Tyr,seq:gta)
     gene            complement(8257..8472)
                     /locus_tag="PK733_11495"
     CDS             complement(8257..8472)
                     /locus_tag="PK733_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOJ11203.1"
                     /translation="MRKKLIIEGMSCSHCVDHVTEALGELPGVESVKVDIDDMSAEVE
                     LTDNIEEEDFRNVIDDIGYELIEVEDI"
     gene            complement(8605..10284)
                     /locus_tag="PK733_11500"
     CDS             complement(8605..10284)
                     /locus_tag="PK733_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11204.1"
                     /translation="MIENIVKKTWLNTVIILIAVISLTFSGCISKVETSDKIMAPQVE
                     GTPLAGQWEIVDILPGKSRGDAEAIEENWTGKKVEMAKDGLLVDNDFWSNISYKIKRV
                     NAEEYFLYRFRQPAKNIDIKDKEIFVINISSEGKFLCEIIKTAEGEAILSIEEQYFRM
                     KKISEKTGEHFKETAKMEIGSRSIYAPDENTTSSSGLLLGIRTPVKAGKGDKEKENID
                     NIQKYIYRTLWISYQYGELRPIFEADDIFLPRISGFWRLGVKERTFENKSEEVLYTHE
                     IVRDETEENIEGNEWDSFWNNKEGIIRREILYAGNDYVSIALTGEGVSKPNDFTYYDT
                     IGRAELKVNNINSRGKIDEKQDYLRWSINRYQTLTVDYIDSFKGIKISDIAGENANLA
                     LQNAIDEVIGTLDSEKVEKIDKTRQDENFALFRKTGHWFFKGRLGLLNNEGSSPNSYQ
                     DFYINLIPPLKLVSYDELHASWTHIKDRIPQALDVYTSPNKDLAIVLTSSSLLIYKIN
                     RKLLSDFPIGIIKLNNGNSVVMAEWATGDYVEKWERAFIMNNNWRKVEIEK"
     gene            complement(10416..11870)
                     /locus_tag="PK733_11505"
     CDS             complement(10416..11870)
                     /locus_tag="PK733_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ11205.1"
                     /translation="MKSARMRIAANFIFIIIITVIILEALIIGIIKQNYYKNLENSLL
                     NQVKISADIYRRYFSDASLNDNVLNNVDTFWKQATAQVEIIDTDGRVLMDSIGMMPPE
                     SARMDDVREALKGGTGKWIGKAYYSRENVISVSYPLKSKDNNIVGALRFISSLKEVDK
                     DVGQVAGGFLLIGIAVVLVSGIISLLLADTIVGPLEKITVAAEKMVDGDFHARCEKKF
                     DDEVGKLSDTLNYLAEEILKKDQLKNDFISSVSHELRTPLTSIKGWAVTLKTMEYADG
                     NKVDCDLDDGSNGSNRNSDREIFIDGLEIIEKESDRLTSMVEELLDFSRFVSGKVTIK
                     KEEVIVENIMHHIERQLMPRAKRENIQLSIDFEDEMPDIITDENRLKQVFINILDNSF
                     KFTTSGGEISFTANILEGDRIRFSIKDTGCGIPSEDLPKVKEKFYKGKNSKSKNGIGL
                     SICDEIIKLMNGTFEIKSEIGKGTEAIVILPVKS"
     gene            complement(12001..>12634)
                     /locus_tag="PK733_11510"
     CDS             complement(12001..>12634)
                     /locus_tag="PK733_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ11206.1"
                     /translation="NLKRNGFTVLEASSGEEALEMVKTYNPEIIILDIMLPGISGFEV
                     CSRIRQDMPEIIIIVLTARGQDIDKITGLELGADDYMVKPFNPLELVARIKSILRRTG
                     NNNDNNNEDRLISQNFVMDNKAQRFFKDESEIELTPTEFLILKTFMENPGKAISRDEL
                     LNAVWGKNYFGDMKTVDVYIRRIREKIEDNPSRPKYIETVWGRGYRWQIE"
BASE COUNT         3832 a         2538 c         1783 g         4381 t
ORIGIN      
        1 tcaacgcttc cgaggttagc tgacggattc ggactgaaag gaattagccc taccaaaaaa
       61 catcagataa aattctaact caatgttttt ggattcaccc caatttatat ggttcccccg
      121 tatcctgcat aaaattacag gaattcagca attaaatatt agttttagtt atatatttgt
      181 ctctttgtat acaaatgaca ttaatatatt ttctaattat attattttat acttttttcc
      241 aaaataatgc aaacactata atgtgttatt attaatattt atagttaatg ttatagtgta
      301 tactaatact ttcttgcata tcctttattt ttcaatcacc gtttcaataa atacatctag
      361 ataaaaccgc catggatctt catggtttta tttcctttgt tgtatttatt ataacataag
      421 gcaattcgcc aatcgaattg cttcacaccg tttcaataaa tacatctaga taaaactgcc
      481 atggatcttc atggttttat ttcctttgtt gtatttatta taacttatct agaaaatttt
      541 tcttcaatta attttgctgt tttgtcttta tcatatatat aataccataa attgtctgta
      601 tataccgagt ctcctggcag agtatatgct ttaatatttg taatattgat tttatttatg
      661 tttcttaaat agtacctaaa atcgtttaga ctgatatttg ttcttgcatg ctcttttaat
      721 atgctaaata tttcatctgt tttggaaaaa tacctcaggt tgaatttctg ttgtatcaag
      781 gctttaataa atttctgctg cgcctctatt ctccctaaat caccatctat atacccctgt
      841 ccgggattgt tgcctttacg gtatctaaca aattcctctg ctttttcacc gttcaatacc
      901 tgtatgcctt tttgcagatt aatatgtaaa ttctggacag gatcatcata tttcatatgg
      961 aaaggtacat caatttcaac tccgcctaat tcatctacta tttttctaaa tgcaagaaaa
     1021 cctatagtaa tataataatc tacaggcagc ccggttatct cctcaattct agatattaac
     1081 agcttttctc ctcccatacc aatcattgca ttaattttct catccgcacc gttaatatgc
     1141 actttagtat cacgggaaat ggataatata ttaattgtac tttcccaggg cttatagttc
     1201 acaagtatta ttacatccga cctaaccatg tcttcatcca agccaagtat aagaaggttt
     1261 acaggaattt catcaatttc gccttcattc ccgttcctat tcttgttatc catatttccc
     1321 gaatattcat cggaattatt attctttttc tcctgtattc ggcttttcat ttcctgctca
     1381 agttcctggt ttgcaaaatg gattatggca attccatcta taaacaggat gggaattaca
     1441 aatatgcaaa ttatcatgat catttttttt aaaatcatat catccaccat cgcagtcgct
     1501 ataatttacc tatataatat tatgtcttat atttgagcat ataatactgt caagttaatg
     1561 actggtgcaa gacgcatttt caatttttga tgtgaaaaag gcgctgaact cagcgccttt
     1621 tttatatgat cacttttatg caatttctac ttcctgttga tttgttaata ttttaatcat
     1681 ttaatccagt tttaatctta ctttcaaccg ctcaatagcc tccattgtac tctctctgct
     1741 tccgaatgcg gttaatctga aatatccctg accgcttggt ccgaaaccta ctccaggtgt
     1801 tccaacaata ttggcttcgt ttagcagttt gtcaaaaaat gtccaggaat ccatgttgtt
     1861 tggggtctta agccagatat atggagcatt cactcctccg aaaacctgca aacccaaact
     1921 taaaaggcct tctctgatga ttttggcatt ggtcatataa taatcaataa gagactttat
     1981 ttgtttctgc ccttcatctg tgtaaactgc agctgctcct ttttgaataa tgtatgaaac
     2041 accgttaaat ttagttgtct gtcttctgtt ccatagttta tttaatggaa cagcatctcc
     2101 tttttccgta taagccataa cagttttagg tacaactgta tatgcacacc ttgtaccggt
     2161 aaaacctgca tttttggaga agcttctgaa ctcaattgct acatcctttg ctccttcgat
     2221 ttcataaatg ctatgaggta tgcctttttc ctgaatatat gcttcatatg cgccatcgta
     2281 taatattaca gctttattct ctcttgcata gtcaacccat tttttaagtt cagactttgg
     2341 aagggtcatc cctgttgggt tattaggata gcaaaggtat atcatatcaa ccttttcctt
     2401 tggcaattca gggataaaat tgttctcagc attgcatgaa atatatacaa ccttttcaaa
     2461 tttgccggtt tcacttaatg aacctgttct tcccgccata acatttgtat caatatatac
     2521 aggatataca gggtctgtaa cagcgattat attatcaagc ccgaatatct cctgaatgtt
     2581 gccggtatca cattttgaac catcacttat aaaaatttca tcttcatcaa gtgatattcc
     2641 taaaggtttg taatcatatt caataacttt ctgaataaga aatttgtagc cctgctcagg
     2701 tccataaccc ttaaaggttt tttcatgagc catttcatct acagcttcat gcaagctttt
     2761 tattacactc tcaggtaagg gtctggtaac atcacctata ccaagacgta taatatccgc
     2821 ttcaggatgt tcatctttaa acctggcaac acgtcttcca atttcagcga aaaggtagct
     2881 tccaggaagt ttcaaataat tctcattaac tcttaccata attttcccct ctcagcaatt
     2941 ttttgagtaa tactttcagt agcattttca gcaatatttt cagcaatatt atttattcta
     3001 taaatgactt atatcaatgc gaccctcaaa gacctttgta gcaggcccgg tcatataaac
     3061 atggttatct ttttctttcc actcaataac cagatctcct ccaagcaact taatagtagc
     3121 ttttctttca cttacattat taagtacgga ggcaacaaga acggcagatg cccctgtacc
     3181 gcaggccatg gtttctccgg ctcctctttc ccacacccgc atcttgagag tacttctatc
     3241 tatgacctgt acaaactctg cgtttatttt tctggggtag agatcatgtt tttccatttt
     3301 aggtcctatc acctcaagcg gaaaattcat aacatcatct acataagtaa tggcatgagg
     3361 atttcccatt gatactcctg ttgctttata tgttctccca tcaatttcaa cgggttcgga
     3421 tataaacttt tccttatcac tttttattgg tatatcccgc ggcaaaagaa ccggttctcc
     3481 catatcaaca gtcacaagca ccacctgatt atattctgtt accatatcaa ggaatttaat
     3541 tccagcaagt gtttctatgc ttactgcagt tttttgagta agcttgttat cataaacata
     3601 ttttccaaca caacgtattg catttccgca catttccgct tcagacccat ctgaattaaa
     3661 cattctcatc ctgaaatccg ctgctttgga aggcataata agtacaagtc cgtcagagcc
     3721 aattccaaaa tgtctgtcac taataaattt agcaactgct gacggatttt caattatctt
     3781 ttcaggacat ttatctacgc agttcacgta tacataatca ttgcccaatc catgcatttt
     3841 tgtaaattct atcatacatc aacctcccgc tgtatattat aacatcagtg caatccgcca
     3901 atcggattgc ttcacaccgt ttcaataaat acacaaagat aaaaccgcca tggaacttta
     3961 tggttttatt tcctttgcag tatttattat aacatcagtg caatccgcta atcggattgc
     4021 ttcacaccgt ttcaataaat acaaaaagat aaaaccgcca tggaacttta tggttttatt
     4081 tcctttgcag tatttattan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     4201 aacatatccc ggggtatggc tgcaaacaaa ttaacgtaca tgaaaagagt ttcaccctgc
     4261 cctaatgcag aaccctaact aacatcactt actaataatt tcttctaatg gttctacttg
     4321 ggtgcatgat acctgtgcca ttgtttttac cggccttgcc catataactg catttttaat
     4381 aactcttaag atatcctgat tataatatat tgggaaggtt tcatgcccag gctggaaata
     4441 aaatacctta ccatagcctc tgttataaca gcaaccactt ctgaacactt cccctccctg
     4501 gaaccatcca ataaatacaa gggtatccgg ttcggggata tcaaaccttt ctccatacat
     4561 ttcttcatgc tccaattcta tgtattctcc tattccttca gcaataggat gtccaggttc
     4621 aactacccat aatctttcct tctctgcgat ttctctccac ttgagggtac aacttgttcc
     4681 catcaattta gtaaatattt ttgacatatg ggaagaatga agtgcaataa ggcccatccc
     4741 gcaaagaact ctattataaa tcttattaac aatctcatca tttacttttt catgagcagt
     4801 atgtccccac cagatcagaa catccgtatt attaagcacc tcatccgtga gtccatgctc
     4861 aggttcttct aaagttgctg ttctaacctc cagttcatca tattgcttta ggtatgcggc
     4921 aatggctcca tgtataccct cagggtatat ttcggctggt ttcccgcccc ttttttcatg
     4981 cctgtactca ttccacactg ttactctgat tttcatataa tttaccccct ttgaaaaatt
     5041 aatgtaacgg attacattga atattgtaac ctataaagta gaaatgttca ataaacatta
     5101 taaaaattaa aagtctctac tatatcctcc acttatacgg cttatcaata ttttcttcaa
     5161 ataaaatacg ctcaaattta agctattatg atatttatgc acccgaagaa cttcacctga
     5221 tgcattttta tataaaaaat aaggcataat taatatatat tgtttcaagc ggagaagatt
     5281 ggagtgtttt atttggatat acttattata attcctgcat ttaatgaaga aaaatcaata
     5341 acatcggtaa ttagtcaaat ccggcttcat atacctgaag ctgatatttt agttataaat
     5401 gacggttcta cagattctac cggcgaaagg gcaaaagctg caggagcaaa tgttgttaat
     5461 ttgcctttta accttggtat tggcggagct atgcagacag gatatctgta tgcaagatat
     5521 aataattatg atatagccat acaagttgat ggggacggtc aacatgatcc ttcatacata
     5581 catcaattaa taaagcctgt attagaagac tatgccgaca tggttattgg ttcaagatat
     5641 attcaggaaa cagcttataa atcttctgca gtaagaaggg tgggaatggt atttttctct
     5701 ctgcttgtta aatttctgac tgatcaacat gtgaaagata caacatcagg gttcagagcc
     5761 attaacagaa atattatcaa ctatttttca ttaaattacc ctgtagacta tccggaagtt
     5821 gatgttcttg tgaaacttaa aagaaaaaaa tttaaggtaa tggaaatacc tgtggaaatg
     5881 aaaacccgta aaacaggtaa atcatcaata actcccatac gttcaatata ttacatgata
     5941 aaagttacac tatcattgtt aatagagact gtaaaaacag tagaatattg aaaaaggtgt
     6001 gaataggtta tgattttaaa tgtatactcc atttcaatag cttttagtgt tgtctttctt
     6061 ataatagtaa tagagcttgt caggaaaaac aaactgcagg aaagatattc tcttttatgg
     6121 attttaatga gtattgtatt attgatactg tcatcaaccc ctgtaataat aaatactatt
     6181 gcagattggc tggaaattaa aaatcctcct tcctttctat ttctctttgg gatggtatat
     6241 ctaattatct ataaccttca cattactact gtagtttcaa aacaatccga aagaatcacc
     6301 aaactggcac aagaaattgc cctgttaaaa gagaaaaatg aaatatagaa aatgcaattc
     6361 aacggacatt aatgctttat aaactaattt tatgtggtaa aatcaataca tatcaataat
     6421 tattaacaac attttttagg aggtgtgtgt tttatgggat caataaaaat tgtaaaaaag
     6481 gagtataagg gttgggaaaa ctgtgttgaa ttatctaacg ggcttgtgga catcatcgct
     6541 gttactgata ttggaccaag gattattcga tttggtttta ccggtaaaga aaatgaattt
     6601 tgtgaagttg cagatcaggt aggtacaaag ggaggagacg aatggaaaat ttacggaggc
     6661 catagattat ggcacagtcc cgaacataaa ccacgtagct atgaaccgga taattcaaaa
     6721 ataaactatt acgaaaagga agatggcatt gtactgtgtc agcctgttga acaaagagca
     6781 ctaataaaaa aagaaattga aatctctcta tctcctgacg aagcaaaagt tacaatcttg
     6841 cacaggctta ctaataaagg cttgtggcca attgagcttg cagtatgggc attaacagtt
     6901 atggcccccg gaggcatgga aataataccc caagctaata gagagaccga tttacttcct
     6961 aatagaatgt tatctctgtg gccttataca aagttaaatg atcccagggt atattggggt
     7021 gaaaaataca taacattgca gcaggacagc agtataaata cacctttcaa gataggaatg
     7081 tccaatgagc atggctgggc ggcatatgcc aatcatggca atctttttgt aaagcagtac
     7141 aatcatgtac ttaatgctgt atatcctgat tttggagcat cttcatatga aacatatacc
     7201 tgtgacttta tgacggaaat ggaatcccta agtcctatgg taacacttga accggatgaa
     7261 tatattgaac ataccgaggt ttggaccctt ttccacggag tcaaagctcc gcaaaacgaa
     7321 aatgaaatag ataatttaat cctgcctctc ataccagatg caaaaagata attttatctg
     7381 gaaaccaata ccaaaggaga acaaatatta taaagtagat gcatactgat aatagataaa
     7441 tactactatg atagaaagtt accaggagga aaaactttag aaaatgacaa taaaaatgat
     7501 gacaaaattg aaatcaaagg acactatacc gcagttttta aggtatataa caacaggagg
     7561 tctggctttt atacttgaat attctacctt ttacatactg agcaatttgc ttgatttctg
     7621 gtacatatgg gcaaattcaa tagctatgac tcttggcttt gctataagct ttatcttaaa
     7681 caggcattgg tcgtttaaat ccggtgccaa tcctgtaaag cagttgattc tgtatggcat
     7741 tctatttctg ataaatctgg gtatatccaa tctccttatg cttctattta ctggtacaat
     7801 gggtataaag cctcttatat caaaaattat tacaatagga atccttgtat gctggaactt
     7861 cgtaatatat aagaaaataa tctttaaaaa tgagtaaccc aaatgagtaa acttaatggg
     7921 caagcaaaat gcgtaaactg aatgagcaag caaaatgaag aagcaaaatg ataaaacaaa
     7981 atgatagaac cggaaaagaa gaaagcgaaa ccttttaaag gtttcgcttt ttggtggagg
     8041 gagatggatt cgaaccatcg aagtcgatga caacagattt acagtctgcc ccctttggcc
     8101 tctcgggaac ccctccaaaa attttcaatg tgcttagtcc catttcagaa gcacaagcac
     8161 atataataaa atacactgtt tttatatttt tgtcaatact tttatcgaaa attaattttc
     8221 taattttcta aaatattttt ttaatttttt cgctctctaa atatcctcta cttcaatcag
     8281 ctcatatcca atatcatcta taacatttct aaaatcttct tcttctatat tatcagttaa
     8341 ttctacttcc gcagacatat catcaatatc cacttttact gattctactc caggtaattc
     8401 acctaatgct tccgttacgt gatcaacgca atgactacat gacatacctt caattatcaa
     8461 cttttttctc acaataaatt cctccttcaa aattaaatta acttttgtaa ttaatgcagt
     8521 atttcattta aactgtttgc tatattatta tatccacatt taacgataaa ataacaaaac
     8581 tatgaactat tttcactgtg cctatcattt ttctatctcc acttttctcc aattattatt
     8641 cataataaaa gctctttccc acttttcaac gtaatctcct gttgcccatt ccgccataac
     8701 aacagaatta ccattattaa gttttattat tccgatggga aaatctgaca gtaactttct
     8761 gtttatctta tatataagaa gactgctgct tgttagtaca atagccaaat ccttattggg
     8821 agatgtatac acatcaagtg cctggggaat tctatctttt atatgcgtcc aggatgcatg
     8881 aagctcatca tatgatacta gttttaaagg tggaattaga tttatataaa aatcctggta
     8941 tgagttcgga ctgctgcctt cattatttag taaacccaat cttcctttaa aaaaccaatg
     9001 tccggttttt ctaaataagg cgaagttctc atcctgtctt gttttgtcaa ttttttcaac
     9061 tttttcacta tccaatgtac caattacttc gtctatggca ttctgcaaag ccagattggc
     9121 attttctcca gcaatgtctg aaattttaat acctttaaaa ctatcaatgt agtcaacagt
     9181 caatgtttgg tacctattaa tactccacct caaataatcc tgtttttcat ctatctttcc
     9241 cctgctattt atattattca cttttagctc agctctgcca attgtatcat aataggtaaa
     9301 atcatttggt tttgataccc cttctcctgt tagagcaatt gatacataat cattacctgc
     9361 atataatatt tcccttctta taattccctc tttgttattc caaaatgaat cccattcatt
     9421 cccctctatg ttttcttctg tttcatccct gacaatttca tgtgtatata aaacctcttc
     9481 gcttttattt tcaaaagttc tttctttcac ccctaaacgc cagaacccgc taattctagg
     9541 gagaaatata tcatctgctt caaatatggg tctgagttcg ccatattgat atgaaatcca
     9601 taatgttcta tatatatact tctgtatatt gtcaatattt tctttctctt tatccccttt
     9661 tcctgccttc acaggtgtac gtatccccaa taatagtcct gaagaagaag ttgtgttttc
     9721 atcaggcgca taaattgacc tggatccgat ttccattttt gctgtttcct taaaatgttc
     9781 tcctgtcttt tcggatattt tcttcattcg aaagtactgc tcttcaatac taagaatagc
     9841 ctcaccttca gctgttttta ttatttcaca aagaaacttt ccctcagagg aaatatttat
     9901 gacaaatatt tctttatctt ttatgtcaat atttttagcg ggttgtctaa acctatacaa
     9961 aaagtattct tcagcattta ctctttttat tttatagctt atattactcc agaaatcgtt
    10021 gtctacgaga agcccatctt ttgccatttc aactttcttt cctgtccagt tttcctctat
    10081 tgcctccgca tctcctctac tcttgccggg caaaatatcc acaatttccc actgtcctgc
    10141 caatggagta ccttctacct gtggtgccat aattttgtct gatgtttcta cctttgatat
    10201 acacccgcta aaagtcaaag atattacagc aatcaatata attacagtat ttagccatgt
    10261 cttttttaca atattttcaa tcatataaac caccctaaat ctacttgatg cattcaatta
    10321 aatttatcat taacaataca atttattaac aatacaattt attaacaata cgatctataa
    10381 taacgtcgat aataataata ataacataaa tagtattaag atttaaccgg gagaataact
    10441 atagcctcgg tgccttttcc aatctcactt tttatttcaa atgttccatt cattaactta
    10501 attatttcat cacaaataga taaccctatc ccattctttg atttactatt cttgccttta
    10561 taaaattttt ctttaacttt tggcaagtct tcagagggaa ttccacaccc tgtatctttt
    10621 atggaaaaac gtattctgtc tccttccaga atattggcag taaaacttat ttccccaccc
    10681 gaagtggtaa atttaaaaga attatccaaa atatttatga aaacctgttt tagtctgttt
    10741 tcatccgtaa taatgtccgg catttcatct tcaaaatcaa tactaagctg tatattttcc
    10801 ctttttgctc ttggcataag ctgcctttca atatggtgca taatattttc tacaataacc
    10861 tcttcctttt ttatagtaac cttacctgat acaaacctgg aaaaatccag aagttcttct
    10921 accattgatg tcagtctgtc actttctttt tcaattatct ccaggccgtc tataaaaatc
    10981 tctctgtctg aattcctgtt gctaccgtta ctaccgtcat ccaaatcaca gtcaactttg
    11041 tttccatctg catattccat agttttcaat gtaacagccc acccttttat ggatgttaaa
    11101 ggagtacgca gttcatggga tactgatgaa ataaaatcgt tttttaactg atccttcttt
    11161 agtatttcct ccgccaagta atttaaagtg tccgacagtt tacccacttc atcatcaaac
    11221 tttttttcac atcgggcatg gaaatccccg tcaaccattt tttcagctgc aacagttatc
    11281 ttttcaagag gccctacaat agtatctgca agcagtaaac tgataatacc cgataccaat
    11341 actactgcaa tacctatcaa aagaaatcct cctgcaactt ggccaacatc cttatctacc
    11401 tcttttaatg acgatataaa ccttaaggct ccaacaatat tgttatcttt tgattttaaa
    11461 gggtatgaca ccgaaattac gttttcgcgg ctgtaataag ctttccctat ccatttgcct
    11521 gtaccacctt ttaaagcctc cctaacatca tccatcctgg cactttcagg aggcatcata
    11581 ccaattgaat ccatcaatac ccttccgtca gtgtcaatta tctctacttg tgctgtggcc
    11641 tgtttccaga atgtatctac attattaagc acattatcat taagagatgc atcagaaaaa
    11701 tatcttctat aaatatctgc agatatttta acttgattta agagactgtt ttctaagttc
    11761 ttgtaataat tctgttttat aatacctata atcagcgctt ccaatattat tactgttatt
    11821 attattataa atatgaaatt agctgcaatt ctcattctgg cacttttcat aatatccacc
    11881 ttaacaaaat agtatctggc gccacccaaa atatatcaaa aatatttgga gtcatgcctg
    11941 atacagcaaa atgacttttc ggatatggca aaatggcttt cgcctaaatt aacattgctt
    12001 ctactctatc tgccacctat atcctctacc ccatacagtt tctatatatt taggtcttga
    12061 tgggttatct tctatttttt ctctgattct tctaatataa acatctacag tcttcatatc
    12121 gccaaagtaa ttcttccccc atacagcatt tagaagttca tccctgctta tagcctttcc
    12181 gggattctcc ataaaagttt tcaatattaa aaactccgta ggtgtaagtt caatttctga
    12241 ctcatcttta aaaaagcgct gtgctttatt gtccattaca aagttttggg aaataagcct
    12301 gtcctcattg ttattatcat tattattacc tgttctccgt agaattgatt taattcttgc
    12361 aacaagctca agagggttaa aaggcttgac catatagtca tcagcaccca gttcaagtcc
    12421 tgtgatttta tcaatatcct gtcctcttgc tgttaatact attattatta tttcaggcat
    12481 atcctgtctt atcctgctgc acacttcgaa tccactgata ccgggcagca ttatatcaag
    12541 aataatgatt tcaggattgt atgttttaac catttcaaga gcttcttcgc ctgatgatgc
    12601 ctctaaaaca gtaaatccgt ttctttttag attt
//
LOCUS       DAOSDD010000124        12631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1923_length_12631_cov_4.931695, whole genome shotgun sequence.
ACCESSION   DAOSDD010000124 DAOSDD010000000
VERSION     DAOSDD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1923_length_12631_cov_4.931695"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..2135
                     /gene="lepA"
                     /locus_tag="PK733_11515"
     CDS             324..2135
                     /gene="lepA"
                     /locus_tag="PK733_11515"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOJ11207.1"
                     /translation="MASKREKRIRNFCIIAHIDHGKSTLADRLIETTGLLTEREMQEQ
                     VLDNMDIERERGITIKARAVRMIYRHKDGQDYVFNLIDTPGHVDFNYEVSRSLAACEG
                     AVLVVDAAQGIEAQTLANVYLALEHNLEIVPVINKIDLPSAQPDHVKKEIEDVIGLEA
                     ENAPLISAKNRINIQSVLESIVERVPNPEGDEDAPLRALIFDSYYDNYKGVITFVRIK
                     DGKVKVDDEIKMMNTGKEFIVTEVGYLKPDALVQCEELFAGDVGYIAASIKNVRDARV
                     GDTITLAADPAQKPLPGYKKVNSMVFCGIYPADGAKYVDLRDALEKLQLNDAALTFEP
                     ETSVALGLGFRCGFLGLLHMEIMQERLEREYDLDLVTTAPSVIYRITKTDGDVINIDN
                     PTNMPPVTEIEKMEEPVVKASIMTPSEYVGNIMELAQDRRGTLRNMEYIDENRVMMNY
                     EMPLNEIIYDFFDALKSRSRGFASFDYELLGYKESELVKLDIYLNGEIVDALSFIFHK
                     DKAYTRARKMAEKLKDAIPRHQFEIPIQAFIGGKIIARETVKAYRKDVLAKCYGGDIS
                     RKKKLLEKQKEGKRRMRQVGSVEIPQEAFMSVLKLDS"
     gene            2177..3352
                     /gene="hemW"
                     /locus_tag="PK733_11520"
     CDS             2177..3352
                     /gene="hemW"
                     /locus_tag="PK733_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOJ11208.1"
                     /translation="MILNDGELGLYVHIPFCKSKCYYCDFNSYASKEYLISSYCEALI
                     KEIEYYGNLAGTNRKPGEHYNSYQIKSIYLGGGTPTHIPPQFILQIIEKCRNNFFIKD
                     EAEITLEANPGTLSDEKLEVYRNAGVNRLSIGLQAWQDRLLKMLGRIHNVQDFISNVN
                     AAQKLGIGNISVDLIFGLPMQTLDEWFQTLDEVLKLNIMHLSCYSLKVEEDTVLEEKI
                     RKGELPVPDEDIDREMYHNTILKLKSKGFKHYEISNFSKPGFESRHNLIYWKAEEYIG
                     LGAGAHSYFEGKRYNNEVKPEKYISEIQKGNIPMRNIVSLSEKDRICEFLILGLRLTD
                     GVDTQEFKNKFGKDLFELYGANVDILIKRGLLEIEGEIIRLTPLGLDLANQVFIEFI"
     gene            3520..4584
                     /gene="hrcA"
                     /locus_tag="PK733_11525"
     CDS             3520..4584
                     /gene="hrcA"
                     /locus_tag="PK733_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOJ11209.1"
                     /translation="MMLLDDRKRMILKAIIDDYISTAEPIGSRTIARKHEIGLSSATI
                     RNEMADLEYMGLLEQPHTSAGRVPSDKGYRIYVDQLMTERSLTASEIESVKSALKLRI
                     SELGQLIKQASAVVSEITKYTSMAITPQMNKCTIKTLQLIPVEHDKLLIVAVTNAGVI
                     KNSLVKTGCKVSVDSLIKLTNLLNDVLSGLTLEKINLADLMCEAENLLEKEKEIILPV
                     LVGIKDCLNQVFSSEVYLEGTTNMFNFPEFQDLIRARQFLNILDTKEILCTLMYNGIE
                     NGDIKVKIGAENELDELKDFSLITATYSLDYEIIGSIGVIGPTRMQYPKVIASLNYIR
                     REINRELNNLIRENTDDSSR"
     gene            4673..5251
                     /gene="grpE"
                     /locus_tag="PK733_11530"
     CDS             4673..5251
                     /gene="grpE"
                     /locus_tag="PK733_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ11210.1"
                     /translation="MNKEINNDIDNNTIEERKAENPEISKDDLIVENEKLSTELENVK
                     SELEEKLAKCDEYLNMLQRTAAEFDNFKKRTQKEKDALSKEVVCDTVSAFIPVADNLE
                     RAMKAIDNDCNYKTVIEGIELVYRQFMEVLEHLGVEEINCIGEKFNPELHNAVMHVDD
                     EAHGDNEVVEVFQKGYIMKDKVIRHSVVKVAN"
     gene            5318..7192
                     /gene="dnaK"
                     /locus_tag="PK733_11535"
     CDS             5318..7192
                     /gene="dnaK"
                     /locus_tag="PK733_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOJ11211.1"
                     /translation="MGKVIGIDLGTTNSCVAVMEGGEPAIIANAEGNRTTPSVVAFSK
                     TGERLVGQVAKRQAITNPDRTIISIKRDMGSNRKIRIDNKDYTPPEISAMVLQKLKAD
                     AESYLGETVTQAVITVPAYFSDSQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKQ
                     HDQKIMVYDLGGGTFDVSILEIGDGVFEVLATSGNNKLGGDDFDQRVMDYIADNFKKE
                     NGIDLRNDKMAMQRLKEAAEKAKIELSSVPSTNINLPFITADASGPKHLDMTLSRAKF
                     EELIADLVEKTMGPTRQALGDAGLTPDKVDKILLVGGSTRVPAVQEAVKKYIGKDPFK
                     GINPDECVAIGAAIQAGVLSGDIKDLLLLDVTPLSLGIETLGGVFTKLIERNTTIPTK
                     KSQTFSTAADGQTSVEIHVLQGEREMAAYNKTLGRFQLTGIPPAPRGVPQIEVTFDID
                     ANGIVNVSAKDLGTGNEQKVTITASSNLSKDDIDMAVKEAERYAEEDKKKKEEIEIRN
                     NADSLVYQSEKTLKDMGDKLGGDDKSKIETEIEKVKSALKGSDLEAIKNATDALQKVF
                     YDVSSKIYQQQGQPGGGAGFDPSGFNPGAGQDGNANPGQDGNVYDADYKVVDDDKEDK
                     "
     gene            7384..8535
                     /gene="dnaJ"
                     /locus_tag="PK733_11540"
     CDS             7384..8535
                     /gene="dnaJ"
                     /locus_tag="PK733_11540"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOJ11212.1"
                     /translation="MANKRDYYEILNVDKNASDDEIKKAFRKLAKKYHPDVNQGNSEA
                     EGKFKEVNEAYEVLGDPQKRAKYDRFGHSAFEQGGFDGGFGGFEGFGGFNDIFETFFD
                     GAFGGSGFSSRSSRSRKSPQRGADLKYRIEVTFEEAAFGTEKEISVTRTEICDNCEGS
                     GAKPGTGLETCKHCNGTGQIQYKHNTPLGSFMSIKTCDVCRGEGKIVTDPCDTCGGNG
                     RVRKNVKMSVNIPAGINNGQTISLRGEGEPGYKGGPAGDLYIDIFVKPHPIFQRQGND
                     IMCEIPVTFVQSALGAEIDVPTLEGTLKYNIPEGTQTGSVFKIKGKGIPNIRGIGRGD
                     LFIKVNIEIPRKLNEKQKAVLREFAAISGDEVYEQRKSFFDKMKNALGL"
     gene            8584..9606
                     /gene="prmA"
                     /locus_tag="PK733_11545"
     CDS             8584..9606
                     /gene="prmA"
                     /locus_tag="PK733_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOJ11213.1"
                     /translation="MKWIEITIETNREACDAISDMLITIGAGGVAVEDPNDIFGEIAG
                     TQYSGSIYEYVDENILNKFNGIFNAGEIVTIKSYFPESSNSVELMQLIKEKLSDISQY
                     LNTGDLKLRCSEVKDEDWANNWKKYYNTFNITKKVVIKPTWEEYEEQDGVIVIEMDPG
                     MAFGTGTHETTKMCAQMLENYINAGDTVIDVGSGSGILSIIAAKHDARNVIALDIDKE
                     AVRVTIENCSRNNVLDRVAAFQGTLSDVNAFDTPGFKEKINEISEGRKADLIVANIIA
                     SVIIDLSDKIDKFLKPGGLIVASGIIKEKKDMVLDSYEPRGFKCIDLMEDGEWVAIVF
                     KCPDSL"
     gene            9588..10361
                     /locus_tag="PK733_11550"
     CDS             9588..10361
                     /locus_tag="PK733_11550"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOJ11214.1"
                     /translation="MSRFFITDDNIYSDSIIISGEDFNHIKNVLRYKTGDSLILCDGN
                     GRDYYVYIEFIGDKEIKTKIIDVSENQTEASVNVTLFQGMAKGEKMDFIIQKSVELGV
                     SRIVPVITERTVVKLENSRDKEKKRIRWQRIALEAAKQCNRGVIPEVGLLVDFTVASD
                     LMHKFNLALIPYEKEEKLALKTVLLEFKNKYSNLDKDKYPEIALFIGPEGGFSDKEII
                     RSHEKGIIPVTLGPRILRTETAALTVQSIIMYELGDIIG"
     gene            10446..11324
                     /locus_tag="PK733_11555"
     CDS             10446..11324
                     /locus_tag="PK733_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOJ11215.1"
                     /translation="MIRSMTGYGRGVAQEAGKIFNVEVKTVNHRYSDISVKMPKQISF
                     MEDRVRSIVSRSLSRGKIDIFISYENSAEGSKKVIVDEILAKEYIYAIRHLRDEYNLA
                     DDITVSLIARFPDVIKVETAEEDEEKLWDILKRAIVSSLDSLIEMREREGLKLKEDLL
                     VKCDFISKSLSEIKKRAPNVVKEYGQKLQNRISELLDQTIVDETRIAMETALFADRCS
                     IDEEIVRLESHIQQLGETLNINEPIGRKLDFLIQEMIREANTIGSKASDLAIIREVLN
                     IKSEIEKMREQIQNIE"
     gene            11341..11622
                     /locus_tag="PK733_11560"
     CDS             11341..11622
                     /locus_tag="PK733_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOJ11216.1"
                     /translation="MKLINIGFGNIVSASRIIAIVSPESAPIKRIIQEARDRGMLIDA
                     TYGRRTRAVIITDSDHIILSAVQPETVAHRLSQKDTIDVVADDAEFIDE"
     gene            11643..12251
                     /gene="gmk"
                     /locus_tag="PK733_11565"
     CDS             11643..12251
                     /gene="gmk"
                     /locus_tag="PK733_11565"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOJ11217.1"
                     /translation="MVHRGLLVAVSGPSGTGKGTVIKALREKNNNITLLPSATTRSPR
                     YGEKDGVNYYFKSRDEFLKMIKNGEFVEWVEYCGNLYGTPAKQLNESLEMGLDVVLEK
                     EVDGVLKIKKAYPDSVSVFILPPSFDELKRRIQGRGTETAEAIQKRLDRALEEMKYAV
                     DYDYIVVNDFIESASNAVNCIINAEKLRTKRNCDIIKLIISE"
     gene            12281..12487
                     /gene="rpoZ"
                     /locus_tag="PK733_11570"
     CDS             12281..12487
                     /gene="rpoZ"
                     /locus_tag="PK733_11570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOJ11218.1"
                     /translation="MIFPSIGSLMEKVDSKYTLAVVAAKRARQLTEGAPRLSNCSSEK
                     EVTIAIYEINEGKISYVRTKNVIK"
BASE COUNT         4508 a         1785 c         2868 g         3470 t
ORIGIN      
        1 agcctttaaa ttcagctacg ggcaaaatca aactcacaaa ataatatgaa taaaagtttt
       61 gtaattattt tgttcaaaca ctgattttgc tcctccttta ttttctttac ttaaataata
      121 tgtaccccta tgatattaac ctgctatatc cactgtaatt ataaaccagg tattgacgtt
      181 gaatgagttt ttatgtcata atcacactat aatattagtt tgttcaagta taacttcgga
      241 tattttaata ttagctggaa atagttggta cgagatcaca atagttagta ttgattggta
      301 atcggaatga aaggaatgga cagatggcaa gcaaacgtga aaaaagaata agaaattttt
      361 gtataattgc ccatatagat catggcaagt ccactcttgc cgacagattg attgaaacta
      421 caggcctttt aactgaaaga gaaatgcagg aacaggttct ggacaacatg gatattgaga
      481 gggaacgtgg tattacaata aaagcaaggg cagtaagaat gatttatagg cataaggatg
      541 gtcaggatta tgttttcaat ttgattgata cgcccggtca tgtagatttt aattatgaag
      601 tttccaggag ccttgctgcc tgcgaggggg cagtattagt tgtggatgcc gcccagggca
      661 tagaggccca gacattagcc aatgtctacc ttgctcttga gcataacctg gagatagtac
      721 ctgtaataaa taaaattgat ttgcccagtg cccaacctga tcatgtaaag aaggaaatcg
      781 aagatgtcat aggtctggaa gcagaaaatg ccccgcttat ttctgcaaaa aatcgtatta
      841 atattcaatc tgtattggaa agtatagttg aaagagttcc aaatcctgaa ggtgatgaag
      901 atgctccatt aagagccctt atattcgact cctactatga taattacaaa ggagttatta
      961 cctttgtaag gattaaggat ggcaaagtaa aagttgatga cgaaataaaa atgatgaaca
     1021 cagggaagga attcatagtt actgaagttg gctatttaaa acctgatgct ttggtacaat
     1081 gtgaagaact ctttgcggga gatgttggtt atatagctgc aagcattaaa aatgtaaggg
     1141 atgcaagggt tggggataca ataactctgg cagccgatcc tgctcagaag cctttacctg
     1201 gctataaaaa agttaattca atggttttct gcggaatata tcctgcagac ggggcaaaat
     1261 atgttgattt gagagatgct cttgagaaac tccagcttaa tgatgctgct ttaacttttg
     1321 aacctgaaac atcagtagct cttgggctgg gcttcaggtg tggttttctt ggattactcc
     1381 atatggagat tatgcaggaa aggctggagc gggagtatga tttggacctt gttactactg
     1441 cgcctagtgt tatttacaga ataacaaaaa ccgatggaga tgtaattaat atagataatc
     1501 caacaaatat gcctcctgta actgaaatag aaaaaatgga agagcctgtt gttaaagctt
     1561 cgataatgac accttctgaa tatgttggaa atattatgga gctggcccag gataggcgtg
     1621 gcacattgag aaatatggaa tatattgatg aaaacagggt tatgatgaat tatgaaatgc
     1681 ccctaaatga aataatatat gacttttttg atgcattgaa atcaaggtcc agaggatttg
     1741 cttcctttga ttacgagctt ttgggataca aagaatcgga acttgtaaaa cttgacatat
     1801 atttgaacgg agaaattgtg gatgctcttt catttatttt ccataaggac aaggcttata
     1861 ccagggcaag aaaaatggca gaaaagctaa aagatgcaat accaagacat caatttgaaa
     1921 ttccaataca ggcatttata ggtggaaaga taattgccag ggaaacagta aaagcttaca
     1981 gaaaggatgt tttggccaag tgctacggtg gtgatataag tcgtaagaaa aagcttctcg
     2041 aaaaacaaaa agaaggcaaa cgccgcatga gacaggtagg ttctgttgaa attcctcagg
     2101 aagcattcat gagtgtgctg aagctggatt cataatgata agaagtggga agtggaaagt
     2161 gggaagtgag aattgaatga tattgaatga tggggaatta gggttatatg ttcacatacc
     2221 cttctgtaag tcaaagtgct attattgtga ttttaactct tatgcttcaa aagagtatct
     2281 aatatcttca tactgtgaag ctcttattaa agaaattgaa tattacggga acctggcagg
     2341 tacaaataga aagcctggtg aacattataa cagttaccaa attaaatcca tatatctggg
     2401 cggtgggaca ccaacccaca ttcctcccca atttatattg caaatcatag aaaagtgccg
     2461 taataatttt ttcattaagg atgaagctga aataacacta gaagcaaatc caggaacttt
     2521 gtctgatgaa aaacttgaag tttacagaaa tgccggcgtc aataggttga gcataggact
     2581 tcaggcatgg caggatagac ttttgaagat gcttggaaga attcataatg tgcaggactt
     2641 tatatctaat gttaatgctg cacaaaaatt gggtattggt aatataagtg ttgatttgat
     2701 ttttgggctt ccaatgcaga ctttagacga atggtttcaa actcttgatg aagtgttgaa
     2761 acttaatatt atgcatttgt cctgctatag cttgaaagtc gaagaagaca ctgtattgga
     2821 agaaaaaatc aggaaagggg agctgcctgt tccagatgaa gatattgata gggaaatgta
     2881 tcataatact atattaaaat taaagagtaa aggttttaaa cactatgaaa tatcaaattt
     2941 ttccaaacct ggttttgaaa gcaggcataa cctgatttac tggaaagcgg aagaatatat
     3001 tgggcttggt gctggtgcac actcttactt tgaaggcaaa agatacaata atgaagtaaa
     3061 accagagaaa tatatttccg agattcaaaa agggaatatt cccatgagaa atatagtaag
     3121 cttaagtgaa aaggatcgaa tatgtgaatt tttaattctt ggtttaagat tgacagatgg
     3181 tgttgacacg caggaattta aaaacaagtt tggcaaagat ttatttgaat tgtatggagc
     3241 taatgttgac attctaataa agcggggact tttagaaata gaaggagaaa taattagact
     3301 tactccattg ggtttggacc ttgccaacca ggtttttata gaatttatat agttgatttt
     3361 atatagataa catacaaaag caactgcttt gttccttgta aaatcatttt tgcgttatat
     3421 atagatattt aaaaaactct tgacaaaaaa aactactggt ggtattttaa atatagtatt
     3481 agcactcgat ataaacgagt gctaacaaga ggagtgttga tgatgttact ggatgacaga
     3541 aaaagaatga tattaaaagc aatcatcgat gactacatta gcactgcaga gcctataggc
     3601 tcaaggacta ttgctcgtaa acatgaaatt gggttgagtt cggcaacaat aaggaacgag
     3661 atggccgacc ttgaatatat gggcttgctt gaacagcctc atacatcagc agggagagta
     3721 ccatccgaca aggggtacag aatttatgta gaccagttga tgaccgagag gtcattaact
     3781 gcctctgaaa ttgaaagtgt aaaaagtgca ttaaagctta gaattagtga actggggcag
     3841 ttaataaaac aagcttctgc agttgtatca gaaattacaa agtatacatc gatggcaata
     3901 acaccacaaa tgaataaatg caccataaag actctgcaat tgatacctgt agaacatgat
     3961 aagctgctca tagttgctgt tacaaatgca ggtgttatta aaaacagtct tgttaagact
     4021 ggctgtaaag tatctgtaga ctctttgatt aaacttacaa acttgcttaa tgatgtactt
     4081 tcaggactta cccttgagaa gataaacctt gcagatttaa tgtgtgaagc agaaaatctt
     4141 ttagaaaaag aaaaagaaat cattttgcct gttttagtgg gaattaaaga ctgcttgaac
     4201 caggttttta gttcggaggt atatcttgaa ggtacaacga atatgtttaa ctttcctgaa
     4261 tttcaggatt taataagagc aagacagttt ctaaatattc tagatacaaa agaaatttta
     4321 tgtactctga tgtataacgg aattgaaaac ggagacataa aggtaaagat tggtgcggaa
     4381 aatgaactgg atgagctcaa agacttcagc ctgataactg caacatacag tctggactac
     4441 gaaataatag gttctattgg agttattgga cccacaagaa tgcaatatcc aaaagttatt
     4501 gcatctctta attatataag gagagaaatt aacagagaac ttaacaacct tatcagagaa
     4561 aataccgacg attccagcag gtaaatatcg ggttttaggt tcgcacagca gttatgtttt
     4621 tacattattc aggttgccga aaaaaattat taaataaggc ggtgaaatac ttgtgaacaa
     4681 agagattaat aacgatattg ataacaatac tattgaagaa agaaaagctg aaaatcctga
     4741 aataagtaaa gatgatttga tagttgaaaa tgaaaaatta agtactgaat tagagaatgt
     4801 taaaagtgaa cttgaagaaa aattagctaa gtgcgatgaa tatttaaata tgctgcaaag
     4861 gacagctgca gaatttgaca attttaagaa aaggacacaa aaagagaagg acgcattaag
     4921 caaggaagtt gtttgcgata ctgtttcagc atttattccg gtagccgaca acctggaaag
     4981 ggccatgaaa gcaattgata atgactgcaa ttacaagact gtaatagaag gaatagagct
     5041 tgtttacaga cagttcatgg aagtgctgga acatttaggc gttgaagaga taaattgcat
     5101 aggagagaaa ttcaacccgg aactgcacaa tgcagttatg catgtggatg atgaagcaca
     5161 tggggataat gaggttgtgg aagtattcca aaaaggttat attatgaagg ataaagtaat
     5221 aaggcatagt gtggtaaaag tagcaaacta aacaaagtat actaaaaata aatacaagct
     5281 ttatgaaagc tttatataaa acaggaggta gaaaaatatg ggtaaagtaa ttggtataga
     5341 tttgggtaca acaaattcat gtgttgctgt tatggaaggc ggagaacctg ctattatagc
     5401 taatgcagag ggaaatagaa ccactccgtc agtagtggct ttttctaaaa caggtgaaag
     5461 actcgttggg caggttgcca aaagacaagc tattacaaat cctgatagaa ccataatatc
     5521 aataaaaaga gacatgggtt caaatagaaa aataaggatt gacaataagg attatactcc
     5581 tccggaaata tctgcaatgg tactgcagaa attaaaagca gacgcagaga gctatcttgg
     5641 agaaactgtt acacaggcag ttattaccgt gccggcatat ttcagcgatt cccagagaca
     5701 ggctactaaa gatgcaggta agatcgcagg ccttgaagtg cttagaataa ttaatgagcc
     5761 tactgcagca gctcttgcat atggacttga caagcagcat gatcagaaaa ttatggttta
     5821 cgacctcgga ggcggaacct ttgatgtttc tatccttgaa ataggtgacg gagtatttga
     5881 agtcctggcg accagcggta ataacaagtt gggtggagat gactttgacc agcgggtaat
     5941 ggattatatt gcagataatt ttaaaaaaga aaatggaatt gatttgagaa atgacaagat
     6001 ggccatgcag cggctcaaag aagctgcaga gaaagcaaaa atagagcttt caagtgtacc
     6061 aagcactaac ataaatctac catttataac tgctgatgct agtggtccga aacatttgga
     6121 tatgacattg tccagggcaa aatttgaaga acttattgct gatctggttg aaaaaaccat
     6181 gggacctaca aggcaggctc ttggggatgc cggactgact ccggacaagg tagataaaat
     6241 attattggtt ggtggctcta cgagagtacc tgcagttcag gaagctgtta aaaagtatat
     6301 agggaaggat ccatttaaag gcattaaccc tgacgaatgt gttgctatcg gtgcagcaat
     6361 acaggccgga gttctttccg gtgatattaa agatctcctc cttcttgatg taactccatt
     6421 gtcacttggt attgagactc ttggcggagt gtttacaaag cttattgaaa gaaatacaac
     6481 tatacctacc aagaaaagcc agacattctc tactgctgcc gatggacaga caagtgttga
     6541 gatccatgta ctacagggtg aaagagaaat ggcagcatat aacaagaccc ttggcagatt
     6601 ccagcttaca ggtataccgc ctgctcccag aggtgtaccg caaatcgaag ttacttttga
     6661 tatagatgct aatggtatag ttaatgtttc tgcaaaagat cttggaacag gcaatgaaca
     6721 aaaggtaacc attactgcct caagcaactt atcaaaagat gatattgata tggctgttaa
     6781 agaagcagag agatatgcag aagaagataa gaagaaaaaa gaagaaattg aaatcagaaa
     6841 taatgccgat tctcttgttt accagtctga aaaaacttta aaagacatgg gcgacaagct
     6901 gggcggtgat gataaatcaa aaattgaaac tgaaatagag aaggttaaga gcgctttgaa
     6961 aggctccgat ctggaagcta taaagaatgc aacagatgct ctccaaaaag ttttctatga
     7021 tgtatcgtcc aaaatatatc agcagcaagg tcaacccggt ggaggagcag gttttgaccc
     7081 ttcaggcttt aatcccggag cagggcagga tgggaatgca aatcccggac aggacggtaa
     7141 tgtttatgat gccgactata aagtagtaga cgatgacaaa gaagataagt aaagcgggtg
     7201 gagtaaaatc atttcatttt actatagaaa gggcggataa tttccgccct ttctataatg
     7261 ttaaggggaa atttaatgtt accacaaaac tagttgtata aacctatgtt gttatgttaa
     7321 aataaatgcg gtcggagaaa taaaatcgtt tgggcagatg gtaagtagca aggagaggat
     7381 aggatggcaa ataaaaggga ttattacgaa atacttaatg ttgataaaaa tgcatcagat
     7441 gatgagataa aaaaagcatt tagaaaactg gctaaaaagt atcacccgga tgttaatcaa
     7501 ggtaatagtg aggccgaggg aaaatttaag gaagtaaatg aagcatatga agttttaggt
     7561 gatccgcaaa aaagagcaaa gtatgaccgg tttggacatt cagcttttga acagggtggc
     7621 tttgatggcg gctttggtgg ttttgaaggt tttggcggtt ttaacgatat atttgaaaca
     7681 ttttttgacg gagcttttgg cggctcaggt tttagcagca gaagttcaag gagcagaaaa
     7741 agtcctcaac ggggcgctga tttgaaatat agaatagagg ttacttttga agaggctgct
     7801 tttggtactg aaaaggagat ttctgtaacc cgtactgaaa tatgcgataa ctgtgaagga
     7861 agcggggcca aacccggaac tggtcttgaa acatgtaaac actgtaatgg tacaggccag
     7921 atacaatata aacataatac acctcttggt agtttcatga gtattaaaac ttgtgatgtt
     7981 tgccgtggtg aagggaaaat tgtaaccgat ccatgcgata cttgtggtgg taacggaaga
     8041 gttagaaaaa atgttaaaat gagtgtgaat atacctgcag gtattaacaa tgggcaaaca
     8101 atatcattaa gaggtgaagg agagcctggt tacaagggtg gacctgcagg ggatctttat
     8161 attgatattt ttgtaaaacc gcatccgatt ttccagagac aagggaatga cataatgtgc
     8221 gagatacctg taacatttgt tcaaagtgct cttggagcgg aaattgatgt gcccactctt
     8281 gaaggaacac taaaatacaa tattcctgaa ggaactcaaa caggtagtgt atttaaaata
     8341 aaagggaagg gaataccaaa tataagggga ataggcagag gagatttatt tataaaggtc
     8401 aacatagaaa ttccaaggaa gctaaatgaa aaacagaagg ctgttctccg cgaatttgca
     8461 gctatcagcg gtgatgaggt atatgagcag aggaaaagct tttttgataa aatgaaaaat
     8521 gcgttaggcc tataaaagtt agaagtagaa agcggaaagt gggagtaagg aaggaactga
     8581 tttatgaaat ggattgaaat tacaatagaa acaaacagag aagcctgcga tgcaatatct
     8641 gatatgctta ttactattgg agcaggcgga gtagctgttg aagaccccaa tgatatattt
     8701 ggggaaatag ctgggactca atattccggc tctatttatg aatatgtgga tgaaaacatt
     8761 ctgaacaaat ttaatgggat attcaatgct ggcgaaatag ttacaatcaa gtcgtatttc
     8821 cccgaaagtt caaattcagt tgaattaatg caacttataa aggagaaact gtctgacata
     8881 tcacaatatc tgaatacagg cgatttgaag cttcgatgtt cagaggtaaa agatgaagat
     8941 tgggcaaata attggaaaaa gtattacaac acatttaata taacaaaaaa ggttgttatt
     9001 aaaccaacct gggaggaata tgaagaacag gatggtgtaa ttgtaataga gatggacccc
     9061 gggatggctt ttgggaccgg tactcatgaa accacaaaga tgtgtgccca aatgctggaa
     9121 aattatatta atgcagggga tacggtaatt gatgtgggtt caggctccgg cattctttct
     9181 atcattgcgg ccaaacatga tgcaagaaat gtaatagcac tggatattga taaggaagct
     9241 gtgagagtaa ctattgaaaa ctgtagtaga aataatgtac ttgacagggt tgctgctttt
     9301 caggggactc ttagcgatgt taatgcattt gacactcctg gatttaaaga aaaaattaat
     9361 gagattagtg aaggcagaaa agcagactta atagtcgcca atataatagc cagtgttata
     9421 attgatttgt ccgataaaat tgataaattc ctaaaaccag gtggattaat cgtagcatca
     9481 ggtattataa aagagaaaaa agacatggtg ttagatagct atgaaccaag aggattcaaa
     9541 tgtatcgacc tgatggagga tggagagtgg gtggcaatag tatttaaatg tccagattct
     9601 ttataactga tgataacatt tactctgata gtataataat atcaggagaa gattttaacc
     9661 atataaagaa tgtacttaga tataaaacag gtgattctct gattttatgc gatggaaatg
     9721 gcagggatta ttatgtgtat attgaattta ttggtgataa agagattaaa acaaaaataa
     9781 ttgacgtttc agaaaatcag actgaggctt ctgttaatgt tacacttttt cagggcatgg
     9841 caaaaggcga gaaaatggat tttataatac aaaaaagtgt tgaacttggt gtcagtagaa
     9901 ttgtacctgt aataacagaa agaactgttg tgaagcttga aaatagcaga gataaagaaa
     9961 agaaaagaat aagatggcag aggatagccc ttgaggcagc aaaacagtgt aaccggggag
    10021 ttatacctga agttgggtta cttgtagatt ttactgtagc ttcagatttg atgcataaat
    10081 ttaacttagc cttgatacca tatgaaaaag aagaaaaatt agccttgaaa actgttttgc
    10141 tggaatttaa gaataaatat tcaaacctcg acaaggataa atatcctgaa attgcattgt
    10201 ttattggtcc ggaaggaggt ttttctgata aagaaattat ccggagccat gagaaaggta
    10261 ttattccagt tacactgggg ccaaggattt tgaggactga aacagcagca ttgactgttc
    10321 aatcaataat tatgtacgaa ttaggagata ttatcggctg aaatctcaaa aagactcttt
    10381 aaattggctg agattatgat ataatactta tctgtaaagc gaataataag aacgagggaa
    10441 gatatatgat aagaagcatg acaggctatg gtagaggtgt agctcaggaa gcagggaaaa
    10501 tattcaatgt tgaagtaaag actgtaaacc acaggtatag tgatatttct gtaaaaatgc
    10561 ccaaacagat atctttcatg gaagataggg taagaagcat tgtaagcagg tcattgtcaa
    10621 gagggaaaat tgatatattt atttcatatg aaaattctgc cgaaggatcc aaaaaagtaa
    10681 ttgttgatga aattttggca aaagaatata tttatgcaat aagacatctg agagatgaat
    10741 acaatttggc ggatgatatt acagtatctt tgatagccag gtttcctgat gtaataaaag
    10801 tagaaactgc agaagaagat gaagaaaaac tttgggacat tttaaagaga gccattgtat
    10861 ccagtctcga ttctcttata gagatgagag aaagggaagg gctgaagcta aaggaagatc
    10921 tacttgtaaa atgcgatttt ataagtaaaa gtttgtcgga aataaaaaaa cgtgctccca
    10981 atgtggtaaa ggagtacgga caaaagctgc aaaaccgtat tagcgaatta ttggatcaaa
    11041 caatagtaga tgaaacccgt atagccatgg aaactgcttt atttgcagac agatgcagca
    11101 tagatgaaga aattgtaagg ctggaaagcc atatccagca gcttggtgaa actctcaata
    11161 tcaatgagcc tataggtaga aaattggact tccttattca ggaaatgata agagaggcca
    11221 acactattgg ttccaaagca agtgacctgg caataataag ggaggtttta aatataaaaa
    11281 gtgaaataga aaaaatgaga gagcaaatac aaaatataga ataacgggga ggtattatta
    11341 gtgaaattga taaatatagg tttcggaaat attgtgtcgg caagcagaat aatagccatt
    11401 gtcagtcctg aatctgcacc tataaagaga atcattcagg aggcaagaga cagaggaatg
    11461 cttatagacg caacatatgg gcgcagaacg cgtgcagtca ttatcacaga tagcgaccat
    11521 attattttgt ctgcagtgca gcctgaaact gttgctcaca gattaagcca gaaggataca
    11581 attgatgtag ttgcagatga tgctgagttt atcgatgaat aggaaggggc agtgtggaaa
    11641 ttatggtcca caggggtttg ttggttgctg tgtcaggtcc ttccggtacg ggtaaaggca
    11701 ctgtgattaa agcattaaga gagaaaaata ataatattac tcttcttccc tctgctacta
    11761 caagaagtcc aagatacggt gagaaggatg gagttaatta ttactttaaa tcgagagatg
    11821 aatttttaaa aatgataaaa aatggagaat ttgttgagtg ggttgaatac tgtggaaatc
    11881 tttatggtac tcctgcaaaa caattgaatg agtctcttga aatggggttg gatgtggttt
    11941 tggaaaagga agtagatggt gtcctcaaaa taaaaaaggc atatcctgat agtgttagtg
    12001 tttttattct gcccccctct tttgatgaat taaaaagaag aatacagggg agaggtaccg
    12061 aaactgctga agcaattcaa aaaagacttg atagggcatt ggaagaaatg aagtatgcag
    12121 ttgattatga ttatatagtt gtaaatgact ttattgaaag tgcctcaaat gctgtaaact
    12181 gcataataaa tgcagaaaaa ttgaggacta aaaggaattg tgatattatt aaactaataa
    12241 tttcggagta gcaccggagc aaatgggagg ttaatgtagt atgatatttc cttcaatagg
    12301 ttctttgatg gaaaaggtgg atagtaagta tacacttgca gtagtagctg caaagagagc
    12361 tcgtcagctg actgaaggag ctccaaggct ttcaaactgt tcttctgaaa aggaagttac
    12421 aattgcaata tacgaaataa atgaggggaa gattagttat gtacgtacaa aaaatgtcat
    12481 aaaatagatg tcactacgga agggaggttt aagaatgacc gtcaataaaa caatggataa
    12541 aattgttgca atggctaaaa atagaggttt tgttttcccc ggttccgaaa tatacggagg
    12601 ccttgcaaat tcatgggatt acggtcccgt g
//
LOCUS       DAOSDD010000125        12521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1943_length_12521_cov_5.188031, whole genome shotgun sequence.
ACCESSION   DAOSDD010000125 DAOSDD010000000
VERSION     DAOSDD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12521
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_12521_cov_5.188031"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..924)
                     /locus_tag="PK733_11575"
     CDS             complement(310..924)
                     /locus_tag="PK733_11575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOJ11219.1"
                     /translation="MRKLTREFYNRTSLDVAKDLLGKYLVHIVDGVEVIGRIVETEAY
                     MGPFDKAAHSYNNRRTKRNEVMFGPPGFAYVFAIYGMYSCMNIVAEEAGKPQAVLIRS
                     LEPVAGLEKMAELRYEKSLSDCNKKELTGLTNGPGKLCQSMHITRINNGTDMCGGSFY
                     VAEGLEEQEFQVVSTTRVNIDYADEAIHYPWRFFIKGNKYISQK"
     gene            complement(1088..1915)
                     /locus_tag="PK733_11580"
     CDS             complement(1088..1915)
                     /locus_tag="PK733_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HOJ11220.1"
                     /translation="MIELPEAINIAKQINETVKGKRIINVIAAQSPHKFAWYHGNPQN
                     YHDLLAGKVIGEAAGFGGLVEIKAEDAVILLGDGVNLRFHAEEDKRPQKHQLLIEFDD
                     FTAISASVQMYGGLWCFKEGEFKNTYFLQAKEKPSPLSDKFDKEYFDNIITAPGVEKL
                     SAKALLATEQRIPGLGNGVLQDILFNSGIHPKKKVKTFTDEDKDNLYDSIKSTIADIT
                     VQGGRDTERDLFGCFGGYKTKLSKNTVDKPCPICGTLIKKEAYMGGSIYYCSGCQKN"
     gene            complement(1950..2834)
                     /locus_tag="PK733_11585"
     CDS             complement(1950..2834)
                     /locus_tag="PK733_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ11221.1"
                     /translation="MHYKEAKAILSPNNGMNIYRGCTHGCIYCDSRSKCYNMDHEFED
                     IEVKSNALELLETALRKKRKKCMLSTGAMCDSYMHIETKLNHTRKCLEIIDKYGFGLC
                     IQTKSNRILRDLELLKSINRKSKCVVQMTLTTYNESLCKIIEPAVCTTKERYKVLKIM
                     RDNGIPTVVWLDPILPFINDTEDNLRGVLDYCIKAKVYGIICFGMGLTLRDGNREYFY
                     KKLDEHFPGLKQKYQKKYGYNYEVMSDNNDKLMNIFYSECNKHGIVCSNGEIFKYMHT
                     FEDKKEMEQISMFENIEK"
     gene            complement(2824..4437)
                     /gene="gpmI"
                     /locus_tag="PK733_11590"
     CDS             complement(2824..4437)
                     /gene="gpmI"
                     /locus_tag="PK733_11590"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ11222.1"
                     /translation="MEYKRPVILVVMDGIGINKSEYGNAVKAAYTPTLDNLIRTCLNT
                     DIKAHGTAVGLPSDEDMGNSEVGHNALGCGQIYAQGARLVNESIENGKMYASNTWNTI
                     ISNCIENMSTLHFIGLLSDGNVHSNISHLKSMLKKSKEHGVSKARIHILLDGRDVEVT
                     SALKYVDDLENLLIELNYAGFDGRIASGGGRMKITMDRYEANWNIVKSGWETHVLGIG
                     KQFENAASAIETYREEYNVIDQDLPPFVIANDGMPIGTINDNDSVILFNFRGDRAVEI
                     SKAFDDEHFDKFDRVRWPKVIYAGMLQYDGDLKLPRHYLVSPPDIQNTLSELLVEHGL
                     KQYAVSETQKFGHVTYFWNGNRSEKFDEKLEVFEQIPSDKVPFEQRPWMKVAGITDKL
                     IDAIRSGKYAFLRANFPNGDMVGHTGDFAAAKIAVEAVDLCLGRIINAVDEVGGILVV
                     TADHGNADEMYEKVKDNGTVEHKTPKAKTAHTLNHVPFIVYDKMVKHEIKEGNFGLAN
                     VAPTIVKLLGIEKPEMWEDSIIEDYQYAL"
     gene            4589..4747
                     /locus_tag="PK733_11595"
                     /pseudo
     CDS             4589..4747
                     /locus_tag="PK733_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
     gene            complement(4847..5767)
                     /locus_tag="PK733_11600"
     CDS             complement(4847..5767)
                     /locus_tag="PK733_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOJ11223.1"
                     /translation="MLTREEYIRLSIELNLFFARIAKEHSIFIETSFTAKDARLAQEA
                     DNFKVQFEMLLGEFIPLANGVISPDVAASGELVTPLTLNAEQISQYYTGTQINTGLTQ
                     AEIGLAGSSGFMPIPMLEQRVFMLNQKAIALTTALAQFKSNILNNVLSCRLFTTNYPL
                     LLDHILREARFYIRMLTKLQKREEILTERDLLEQEIFWNRIMAEHSKFIRGLLDPTEV
                     NLISTANNFGNEFDELTQKAVAAHDKAMNLANISNESLEATKKLKDFKATGTKGLIDC
                     SIKAIAYPLLGDHVLREANHYIRILKLFKK"
     gene            complement(6211..6639)
                     /locus_tag="PK733_11605"
     CDS             complement(6211..6639)
                     /locus_tag="PK733_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOJ11224.1"
                     /translation="MVKQISVFLENKSGRLANVTRTLGMNNIDISALSIADTTDFGIL
                     RLIVNDPDKAVDVLEQEGFTVAGTDVLAIAVEDKPGGLSEALTVLENNSIGIEYIYAF
                     VGKCSNEALVILRVDEPHKAIEILGKNNIKVLEQEDVYKL"
     gene            complement(6734..8035)
                     /locus_tag="PK733_11610"
     CDS             complement(6734..8035)
                     /locus_tag="PK733_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ11225.1"
                     /translation="MEYWNPACECMDRDEMTRIQTERLIETVKRVYFNVPSYRNKMQQ
                     KGIEPGDIKTLDDLKKLPFTYKQDLRDNYPYGMFAVPLSEIVRIHASSGTTGKQTVVG
                     YTRKDLDTWAEVTARTLACAGTDKSSFIQIAYGYGLFTGGLGIHYGAERIGASVIPIS
                     GGNTKRQLQIMKDFGTTILACTPSYALYMAEEMEEAGIKKEDLHLKAGIFGAEPWSEQ
                     MRDEIERRLGILAIDIYGLSEVIGPGVASECKYKCGLHIHEDHFIPEIINPETEEVLP
                     PGSLGELVFTTITKEGLPLIRYRTRDITSLIYDICKCGRTSVRMNKVSGRTDDMIIIR
                     GVNVFPSQIESVLLDLGETAPHYQLIVDRVGKLDMLEVRVEMTQYMFSDQVRNIENTE
                     KKIRNAIESTLGISAKVKLVEPKSIERSEGKAKRVVDNRKI"
     gene            complement(8069..8647)
                     /locus_tag="PK733_11615"
     CDS             complement(8069..8647)
                     /locus_tag="PK733_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOJ11226.1"
                     /translation="MANLNIMIVGVGGQGSLLASRIIGNVAMKKSYDVKVSEVHGMSQ
                     RGGSVITYVKMGDKINSPLIEKGEADIIMSFEKLEALRWVGYLKKTGKLIINDQRINP
                     MPVITGKEKYPDDIINVLKEKFSSNVISVDALEIAEQLGNIRVVNMVLIGILAKTSSI
                     EKDIWLQALKEVVPGKFYSVNEKALEIGYIIE"
     gene            complement(8640..10421)
                     /gene="iorA"
                     /locus_tag="PK733_11620"
     CDS             complement(8640..10421)
                     /gene="iorA"
                     /locus_tag="PK733_11620"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOJ11227.1"
                     /translation="MKKLMLGNEAVARGAYEAGVTVATAYPGTPSTEITENIAKYEEM
                     YSEWSPNEKVALEVAIGSSIAGARSICSMKHVGLNVAADPLFTVSYTGVSGGLVIMVA
                     DDPGMHSSQNEQDSRYYARSSKVPMLEPADSSECKYFVKEAYKISEEFDCPVIIRLTT
                     RVAHSQSIVEIEGKEDYKLKDYSKDFAKYVMMPAMARKRHVEVEKRMTALKEFSNKTE
                     INKIEWGRKDIGVITSGISYQYSKEAFGDVSYLKLGMVYPLPTDLIKKFSEEVETLYV
                     IEELEPFIEDEIKKLGIKVTGKDLLPVTGELSASIIREKIPGLKGDKGQSASEDESIS
                     EKIEINTSVPMRPPVMCPGCPHRGVFYVLKKLKLIVTGDIGCYTLGALPPLESIDACV
                     CMGASIGMAHGIEKARGKEFRKKTVAILGDSTFIHSGITGLIDVVYNKGNSTVIILDN
                     SITGMTGHQHNPATGFTIKGEPTKQVDLVKLAHAIGIDRVKVADPYDLKEFEKIVKEE
                     VEADEPSLIIAQRPCALLKHVKFEGPLKINRDKCKMCRMCMKIACPAIVDNGSFIEVN
                     QALCVGCELCTKVCGFNAFEKAGDING"
     gene            complement(10722..12200)
                     /locus_tag="PK733_11625"
     CDS             complement(10722..12200)
                     /locus_tag="PK733_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659075.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOJ11228.1"
                     /translation="MAKFLFLGLFFAVMIIVGIYSRKKVTNVNDFLLGGRNMGPWISA
                     FSYGTTYFSAVIFIGYAGRIGWNFGISATWIGIGNAILGSLLAWLVLAKRTRKMTNDL
                     NASTMPEFFEKRYDSKAMKITTASIIFIFLVPYTASVYQGLGYLFEKAFNIPFIYCMM
                     GMAVLTGAYLMLGGYVATALNNFIQGIIMIAGIVFMLYYIITNPAVGGLREGLSKLGS
                     IDQNLVKPFGGLPVDLLALVTLTSLGTWGLPQMVHKFYAIKDEDAIKKGTVISTLFAL
                     LIAGGAYFVGAFGRLFFIYNNAQVPANPDMVMPLVLQWALPDALYGLIIILVLSASMS
                     TLSSLVLVSSSAITIDLIKGIWSPNIGKKREMLLMRVFCAVFVVLSFIVAVTPNAILT
                     LMSFSWGTISGAFLAPFMYGLFWKGTTRAGAWAGILSGFLCSVGGAMLLGMNAKLAPN
                     IGAVAMIVSLIAVPVVSLLTSKQSENALGKASVSVAETVKTR"
BASE COUNT         3726 a         2699 c         1874 g         4222 t
ORIGIN      
        1 cgacgaccga ttccttcaaa ggagctatct tgatatgttc cctgaagaat ggagcaatta
       61 tatgaggata tttaatccgg agtctgtaca gaaagtcctt gataggtttt ggctggatgg
      121 ataaaggaag aatactccag actatgtatc tactcatcaa ttgaacattg cagatataga
      181 taacgcacaa aagaaactac actaaaaaag ttttgtgcgc ttatgttacc ttaattaata
      241 cgcaaaaatc caatttgttc cttgtaaaac catttttgcg ttatagatag aataatagga
      301 ttaatatacc tatttttgtg aaatatattt gtttccttta ataaagaacc tccatggata
      361 atgaatggcc tcatcagcat aatcgatatt gactctagtt gtagacacta cctgaaattc
      421 ctgttcctct aaaccctcag caacatagaa gctacctcca cacatgtcag tcccattgtt
      481 tattcttgtt atatgcatgg attggcaaag ctttcccggc ccattcgtaa gacctgttaa
      541 ttctttttta ttgcaatctg aaagggattt ttcatagcgg agttctgcca ttttttccag
      601 gcctgcaact ggctctaatg atctaataag cacagcctgg ggtttgcctg cctcttctgc
      661 aactatattc atacaagaat acattccata aatcgcaaac acatatgcaa acccaggagg
      721 tccaaacatt acttcattcc tcttagttct tctattatta taagaatgtg ctgccttatc
      781 aaaaggaccc atataagcct ctgtttcaac tattctacca ataacctcta ccccatcaac
      841 gatgtgtacc aaatatttac ccaaaaggtc ttttgcaaca tctaaactag ttctattata
      901 aaattccctg gtgagctttc tcatatttta ctccatttaa agtagttttt aatgtatagt
      961 ttgtgcatta ttttctatta tatactcatt aaaaatgctt ggtgaatata ctgcatttag
     1021 ttctggtaag cacatctcta taattttcat tactgcatat gagccagcaa gtgccggact
     1081 tattgttcta gtttttctgg catccactgc agtagtatat actcccaccc atataagcct
     1141 ctttttttat aagagttcca catatagggc agggtttatc aacagtgttc ttgcttagct
     1201 ttgtcttata cccgccaaaa cacccaaaca aatctctttc agtatctcgc ccgccttgga
     1261 cagttatgtc cgcaatagtg gattttatag aatcatacaa attatcctta tcttcatcag
     1321 taaatgtctt tacctttttc ttaggatgaa ttccggaatt aaagagaata tcctgtaaaa
     1381 caccgttgcc aaggccaggg attctttgct cagtagcaag taaagctttt gcactaagtt
     1441 tctcaactcc aggtgcagta ataatattat caaagtattc tttatcgaat ttatctgaga
     1501 gtggcgaggg cttttccttt gcttgtagaa aatatgtatt tttgaactca ccctctttaa
     1561 aacaccatag tccgccatac atctgaaccg atgcacttat tgctgtaaaa tcgtcaaact
     1621 caattagaag ctggtgtttc tgaggtcttt tatcttcttc tgcatgaaac ctcaggttta
     1681 ccccatcacc caataatatt acagcatcct ccgctttgat ttctacaagg ccaccaaaac
     1741 ctgctgcttc acctattact ttacccgcaa gcaaatcatg gtaattctgt ggattgccat
     1801 gataccatgc aaatttgtga ggagactgtg ctgcaataac atttatgatt ctttttcctt
     1861 tgacagtttc attaatctgc ttagctatat taatagcttc aggtaattct atcatttata
     1921 tcatcctttc tgatattctc attaaatatt tatttttcaa tattttcgaa catacttatc
     1981 tgctccattt cctttttatc ttcgaaagta tgcatatact taaatatttc accattactg
     2041 cagacaatac catgcttatt acattcacta taaaaaatat tcattaattt gtcattgtta
     2101 tcgctcatta cttcgtagtt ataaccatat tttttttggt atttttgctt aagtcccgga
     2161 aaatgctcat caagcttttt gtaaaaatat tctctattgc catcccgcaa agtcaacccc
     2221 ataccgaaac aaataattcc atatacttta gctttgatac aataatccag aacacctctc
     2281 agattatctt cagtgtcatt gataaatggc aaaatcgggt caagccatac aacagttggg
     2341 attccgttat cacgcataat ttttaacacc ttataacgct ctttggtggt gcagacagca
     2401 ggttcaataa ttttgcataa gctctcgtta taagttgtga gtgtcatttg aacaacacat
     2461 ttggattttc ggtttatgct tttaagtaac tctaagtcac gcagaatacg attggatttt
     2521 gtttgaatac aaagcccaaa gccatattta tctataatct caagacactt tcgtgtatgg
     2581 ttcagcttag tttcaatatg catataggaa tcacacattg cacctgtact aagcatacac
     2641 tttttacgtt tttttcttaa tgccgtttcc agtaattcaa gagcgttact cttaacctct
     2701 atatcctcaa actcatgatc catattataa cacttacttc tgctatcaca ataaatgcag
     2761 ccgtgagtgc agccacgata tatattcatt ccattgttgg gagataaaat tgcctttgct
     2821 tctttatagt gcatattggt aatcctctat aatgctatcc tcccacattt ccggtttttc
     2881 tatgccaaga agcttcacta tggtaggagc aacatttgca agtccaaaat tgccctcctt
     2941 tatttcgtgc ttcaccatct tatcatagac aataaacgga acatgattta acgtatgagc
     3001 agttttagct tttggagttt tgtgctctac agttccgtta tcttttacct tttcatacat
     3061 ctcatctgca tttccatgat cagcagtcac aaccaatatt ccgcccacct catcaacagc
     3121 attgattatt ctaccaagac acaaatcaac agcttcaact gcaattttag cagcagcaaa
     3181 atcacctgta tgtccaacca tatctccatt agggaagtta gcccttaaaa atgcatattt
     3241 acctgatctg attgcatcaa ttagtttatc tgtaattccc gctaccttca tccatggacg
     3301 ctgctcaaat ggcaccttgt ctgaaggtat ctgttcaaat acttcaagtt tttcatcaaa
     3361 cttttcactt ctattaccat tccagaaata tgtaacatga ccaaacttct gtgtttcaga
     3421 tactgcatac tgctttaggc catgctccac caacaactcg gaaagggtgt tttgaatatc
     3481 aggaggactt actagatagt gcctgggaag tttcaaatct ccatcatatt gcagcatacc
     3541 tgcatagata acttttggcc agcgtactct gtcaaattta tcaaagtgct catcatcaaa
     3601 tgctttgctt atctcaacag ctcggtctcc cctgaaatta aaaagaataa cgctatcatt
     3661 atcattaata gtgccaatgg gcataccatc atttgctata acaaaagggg gaaggtcctg
     3721 atctattaca ttatattctt ccctatacgt ttcaattgcc gatgctgcat tctcaaactg
     3781 cttgcctata cctaaaacat gagtttccca gcctgatttg actatattcc agttagcttc
     3841 atacctatcc attgttatct tcattcgtcc gccacctgac gcaatcctac catcaaaacc
     3901 agcatagtta agctctataa gcaggttttc caaatcatca acatacttca gagcagaagt
     3961 aacctctaca tctcgaccat ccaggagaat atgaatgcga gcttttgaaa ctccatgctc
     4021 tttggatttc ttcaacattg atttcagatg agaaatatta gaatgaacat taccatcaga
     4081 aagaagtcca ataaaatgta atgtagacat attttctatg cagttgctta taatagtatt
     4141 ccatgtgttt gatgcataca tctttccgtt ttctatagat tcattaacaa gtcttgcacc
     4201 ctgtgcataa atctgcccgc agccaagcgc attgtgccca acttcgctat tgcccatgtc
     4261 ttcatcagaa ggcaaaccaa cagctgttcc atgtgcttta atatcagtat tgagacaagt
     4321 cctaataaga ttatccagtg taggcgtata tgcagccttt actgcatttc catattcact
     4381 tttatttatt ccaataccgt ccattacaac taaaataacc ggtcttttat attccatata
     4441 ctattctccc tcctgtccaa tatagagcaa atgctcaggt agtaattggc ctgataatct
     4501 taagaattat ttctataatt aaaagtatat catatgctga tactattaaa taatatacaa
     4561 taaaatttct aagcacatta tgcaagatga tgatatccta ttaaatctta tgattcataa
     4621 atatggtatt ccacctcatg gggggctggg tggaggactt gagcggctta ccatgaaatt
     4681 gctgggacta aataatgtaa gaaatgccag cttgtttcca agagatatga accgtgtaac
     4741 accataagta taaacaatag actccaccag gattcatctc aagattctgt gtaagattct
     4801 gttgaagata atatcctggt ggaatattaa tactttgttt aacaaattat tttttaaaca
     4861 attttaaaat cctaatatag tgatttgcct cgcgcagcac atggtcacct agaagtggat
     4921 atgctattgc ttttattgag caatctatca atccttttgt acctgttgct ttaaaatctt
     4981 tcaatttctt tgttgcctct aaactctcat ttgatatatt ggctaaattc atagccttat
     5041 cgtgtgcagc aactgctttc tgtgtaagtt catcaaattc gttgccgaaa ttgttcgctg
     5101 tactgattag gtttacttca gtgggatcaa gaaggccgcg gataaattta gaatgctctg
     5161 ccataatacg gttccagaaa atttcctgtt ccagcaggtc tctttctgta agtatttctt
     5221 cacgtttttg cagtttagta agcattctta tataaaatct agcttctctt aaaatatgat
     5281 ccaaaaggag cgggtaattg gtagtaaata acctacaaga aagtacatta tttagtatat
     5341 tggatttgaa ttgcgctaat gcagtagtta atgctattgc tttttggttt aacataaata
     5401 ctctttgctc cagcatcgga attggcataa aacctgaact tcctgccaat cctatttccg
     5461 cttgtgttag gccagtattg atttgtgttc ctgtatagta ttgtgaaatt tgctctgcat
     5521 ttagggttaa aggtgtaacc agttcgcccg atgcagctac atcaggactt ataacaccat
     5581 ttgcaagtgg tatgaattca cctaacagca tttcaaattg aaccttaaaa ttatcagctt
     5641 cttgtgcaag ccgagcgtct tttgctgtaa aactagtttc tataaaaatt gagtgttcct
     5701 ttgcaattcg agcaaagaaa agattaagct ctatagataa acgaatatat tcttctcttg
     5761 ttaacatata attctccttt ctgcttccaa aaatccggta ttgtcaattc aagcaatcga
     5821 agtaaagcct tcaaacaaat gtacatgccc atttgcatac cattcgaatc ctttatatat
     5881 tttatgttaa gttgtaaaag aatgttactg ttgcaaatat tgaagatctt attgcaacac
     5941 cataatctca tcgtatagct atatagaaca ggttttaaaa catttaaaac ctgcttctgt
     6001 tagaggtgca tacctgcacc taaactcagc atgactcaat gtcgtgttga gtttggttac
     6061 cttgatttta ctcactattt gcaaattctt cattagttct ttcaaatcgt aaattatata
     6121 ggcatgaaaa agccctgacg gtttaatcca tcagggcttt ttcatctaca tttttctatg
     6181 ctatcataat acagtacaga gatatcaaac ttataactta tatacatctt cctgttccag
     6241 cacttttata ttatttttcc ctaatatctc aatggcctta tgaggttcat caactcttaa
     6301 tataacaagt gcttcatttg agcatttgcc aacaaacgca taaatatact ctatacctat
     6361 tgagttattt tccagcactg ttaaagcttc tgataatcca ccgggtttat cttctaccgc
     6421 tatagccagt acatctgtac ctgcaactgt aaatccttcc tgttcaagaa catctactgc
     6481 tttatccggg tcatttacaa ttagtcttaa aataccaaaa tcagtggtgt ccgcaattga
     6541 taatgcactt atatcaatgt tgttcatccc aagtgtcctt gttacattag caagtctgcc
     6601 tgacttgttt tcgagaaata cagaaatttg ttttaccata caatatcact cctcattttt
     6661 taaaaagggt tcattattcc cgggtcagtc tttattacaa tgtactttga ttgcgtacta
     6721 ctaattttat tacttatatc ttccggttgt caactaccct tttggcttta ccctcacttc
     6781 tctcaatgct ttttggttct acaagtttca ctttcgcact gattcctaat gtactttcta
     6841 ttgcatttct aattttcttc tcagtatttt caatgtttct tacctgatca gaaaacatat
     6901 actgagtcat ttcaacacgc acttcgagca tatcaagttt tcccaccctg tcaacaataa
     6961 gttgataatg gggagcagtt tcacccagat cgagtaaaac actttcaatt tgcgatggga
     7021 aaacattaac tcctctgatt ataatcatat catctgttct gccagaaacc ttattcatcc
     7081 ttacactggt tcttccacat ttacagatat cataaatcag tgatgtaatg tccctggtac
     7141 gatatcttat aaggggtagt ccttcttttg taattgttgt aaataccagt tcacctaatg
     7201 aacctggtgg cagcacttcc tcagtttcag gattgattat ttccggaata aagtgatctt
     7261 cgtggatatg cagcccgcat ttatatttac actcactagc gacacccggg cctattactt
     7321 cacttaagcc atatatatca attgccagaa ttccaagtcg tctttcaatt tcatctctca
     7381 tctgctcaga ccatggttct gcgccaaata ttcctgcctt aagatgcaaa tcttcttttt
     7441 taatgcctgc ttcttccatt tcctctgcca tataaagagc atatgaagga gtacaagcta
     7501 aaattgttgt accaaaatct ttcattattt gaagctgcct ttttgtattc cctcctgata
     7561 ttgggattac agacgctcca attctttcag ccccataatg tattccaagg ccgccggtaa
     7621 aaagaccata accgtatgct atctgtataa acgaagactt atcggtacct gcgcatgcaa
     7681 gggtacgtgc tgtaacttca gcccatgtat caaggtcttt tctggtataa ccaactacag
     7741 tctgcttgcc tgtagttcct gaagaagcat gtattcttac tatctcgctt aaaggaactg
     7801 caaacatacc ataaggataa ttatctctta aatcctgttt ataggtaaaa ggtaattttt
     7861 tcaaatcgtc aagagtcttt atatctccgg gttcaatacc tttttgctgc attttatttc
     7921 tatatgaagg tacattaaaa tatactcttt ttacagtttc aatcaaacgc tcagtttgaa
     7981 ttcttgtcat ctcatctcta tccatacatt cacatgcagg attccaatat tccatagtcc
     8041 tctacctcac ttatgctcta tatttctatt attctataat ataccctatt tctaaggcct
     8101 tttcattaac actgtaaaat ttcccgggca cgacctcttt taaggcttgc agccatatat
     8161 ccttctctat tgatgatgtc tttgccagta ttccgattaa aaccatatta accacacgga
     8221 tattacccag ttgttctgct atctctaatg catcaacact aattacattg ctgctgaatt
     8281 tttcctttaa tacatttata atgtcatccg gatacttttc ttttcctgtt ataacaggca
     8341 taggattaat tctctgatca ttaattatca gtttcccggt tttcttcaaa tatccaaccc
     8401 accttaaagc ttcaagcttt tcgaaggaca ttataatatc tgcttctcct ttttcaatta
     8461 agggtgagtt tattttatct cccattttta cataggttat aacacttccg cctctctggg
     8521 acataccatg tacttcagat actttaacat catatgactt tttcattgct acattgccaa
     8581 ttattctgct ggctaataga gaaccctgtc ctcctacgcc tactatcata atattaagat
     8641 tagccattga tatcaccagc cttttcaaaa gcattaaagc cgcatacttt ggtgcaaagc
     8701 tcacaaccca cacatagtgc ctggttaact tcgataaaac tgccattatc tacaatagcg
     8761 gggcatgcaa ttttcataca cattctgcac attttacact tatccctgtt aattttcagc
     8821 ggtccttcaa attttacatg tttcaataga gcgcagggcc tttgagctat tataagtgat
     8881 ggttcgtctg cttcaacctc ttctttaact attttttcaa actccttcaa atcatacgga
     8941 tctgcaacct ttaccctatc tatgcctatt gcatgtgcca gctttacaag atcaacctgc
     9001 tttgtaggtt ctccttttat tgtaaatcct gtagcaggat tatgctggtg gcctgtcatt
     9061 ccagtaatag agttgtcaag aattataact gtagagttcc ctttgttata aactacatca
     9121 attagacctg taatgccgga atgaataaaa gttgaatccc ccaatatggc tactgttttc
     9181 ttccggaatt cctttccacg tgctttctct attccatgtg ccatcccaat acttgcaccc
     9241 atacatacac atgcatctat ggattcaaga gggggcaaag ctccaagagt gtaacagcct
     9301 atatcacctg taacaatcag ttttaacttt ttgaggacat aaaagactcc tctgtgcggg
     9361 caaccaggac acataacagg tggtctcata ggaactgatg tatttatttc aattttttca
     9421 gagattgatt catcttcaga tgctgattgg cctttatcgc cttttaatcc aggaatcttc
     9481 tctctaataa tcgatgcact taattcccct gtaacaggca ataaatcctt tcctgttact
     9541 ttaatgccca gctttttaat ttcatcctct ataaaaggct ccaactcttc aattacatat
     9601 agagtttcaa cttcttctga aaatttctta atcaagtctg taggaagtgg atagaccatt
     9661 cccaatttca aataggaaac atccccaaaa gcttcttttg aatattgata tgaaatgccg
     9721 ctggtaataa caccaatatc ttttcttccc cattctattt tattgatttc tgttttattt
     9781 gaaaactctt taagagctgt cattcgtttt tcaacttcaa catgcctttt tcttgccata
     9841 gcaggcatca ttacatactt ggcaaaatct ttgctatagt ctttaagctt gtaatcttct
     9901 tttccttcga tttcgacaat gctctgtgaa tgtgcaaccc ttgttgttag ccttataatc
     9961 actggacagt caaattcttc acttatctta taagcctctt taacaaagta tttacattcg
    10021 ctgctatctg caggctcaag catgggaacc ttcgaagatc tagcgtaata tctgctgtcc
    10081 tgttcatttt gcgaactatg cattcccggg tcatcggcca ccattattac aagtcctccg
    10141 ctaacacccg tataggatac agtaaacaac ggatctgcgg caacattaag acccacatgc
    10201 ttcatggaac aaattgatcg tgctcctgct atagatgaac caatagcaac ttccaaagcg
    10261 accttttcat taggcgacca ctcggaatac atctcttcat attttgcaat attttctgtt
    10321 atttcagtgc tgggtgtgcc cggataagct gtagctacag taacacctgc ttcatacgcc
    10381 cccctggcaa cagcttcatt acctaacatc agtttcttca ttaataaacc cccgttttta
    10441 gtgtaagggg acaaaccatc ccagggatct gatgcaagga accagaaatt ggaatgtccc
    10501 cttttaatat ttatagtaaa ttaataatta ttatacaatt cgaatttcct gtaaaacttc
    10561 atggtttaca ttttacaaca ttataataag ttttgcaata attacaaaca tttgtgggcg
    10621 cattttaatt tttgaagaaa tatattctag aggctatcgt tacggtgctt cttccaacaa
    10681 ttacttcaaa aaaataatat gcgcctaaca tttgaccttt gttagcgtgt tttaacagtt
    10741 tccgcaacag acacgctggc tttacctaaa gcattttcac tttgctttga tgtaagcagg
    10801 cttactacag gtactgcaat taaggatact atcattgcca cagcccctat attaggtgca
    10861 agttttgcat tcattccaag aagcattgct ccaccgacag agcatagaaa ccctgatagt
    10921 attcccgccc atgcacccgc tcttgtagtc cctttccaga aaagtccata cataaaagga
    10981 gcaagaaaag cacctgatat agttccccac gaaaaggaca tcaatgtaag tattgcatta
    11041 ggtgttacag caactataaa cgaaagtaca acaaaaacgg cacaaaatac acgcataagg
    11101 agcatttcac gttttttacc tatgttcggc gaccatatac ccttgattaa atcaatagta
    11161 attgcagaac ttgatacaag aaccagggat gaaagtgttg acatagatgc tgaaagtacc
    11221 aatattatta taaggccgta aagagcatcc ggcaaagccc actgtaaaac caacggcata
    11281 accatatcag gatttgcagg gacttgcgca ttattatata tgaagaacaa tcttccgaat
    11341 gcaccaacaa agtatgctcc acctgcaatt agtaatgcaa ataatgttga aataactgtt
    11401 ccttttttta ttgcatcttc atcttttatt gcgtaaaatt tatgaaccat ctgtggtaaa
    11461 ccccatgttc ccaaacttgt taaagtaaca agagccaaaa ggtctactgg taaaccacca
    11521 aaaggtttta caagattttg gtcaatagat ccaagttttg ataaaccttc acgtaatcca
    11581 cctacagctg gatttgtaat aatataataa agcataaata ctattccggc aatcataatt
    11641 attccctgaa taaagttatt tagggctgtc gcaacataac ctcccagcat taaataagct
    11701 cctgtaagaa cagccattcc catcatacaa taaataaatg gtatattaaa tgctttttcg
    11761 aaaaggtatc ccagcccttg gtacactgaa gcggtatagg gcacaaggaa tataaaaata
    11821 attgaggctg ttgtaatttt cattgctttg ctgtcgtatc ttttttcaaa aaactccggc
    11881 atagtggaag catttaaatc attagtcatc ttacgggtcc tttttgccag caccaaccat
    11941 gcaagcaaac tgcccaaaat agcgtttcca atacctatcc aggttgcaga aataccaaaa
    12001 ttccacccaa ttctgcctgc ataaccaata aaaataactg ctgaaaaata tgttgtacca
    12061 taggagaagg ctgaaatcca gggtcccata tttcttccgc ctaaaagaaa atcattaaca
    12121 tttgttacct tttttctgct gtatattcct acaataatca ttacagcaaa aaaaagtcct
    12181 aaaaataaga acttagccat ttttctacca tccttccata ctaaatcttt aatttttaaa
    12241 tttacaccgt actacctgcc tacaaggtga gtgaaaacaa tttgatttcc acaactacca
    12301 ttttacaaca aagcaaaaaa aaaataaagc ccttttttaa aaaaggggcg tattttcttc
    12361 ctatgataca aaatatggca gcatacgatt ttctgagtat aaaatttgaa caaaacttac
    12421 gggttgttat gtttaaggca aggaaaaatt cgtaattcac gacacggatg tcgtgagccg
    12481 tcaccaaaac atgtgcttat taacacaacc aaactattaa t
//
LOCUS       DAOSDD010000126        12479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1950_length_12479_cov_5.184884, whole genome shotgun sequence.
ACCESSION   DAOSDD010000126 DAOSDD010000000
VERSION     DAOSDD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1950_length_12479_cov_5.184884"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..746)
                     /locus_tag="PK733_11630"
     CDS             complement(6..746)
                     /locus_tag="PK733_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOJ11229.1"
                     /translation="MKTLTNGLIKENPSLRLVLGTCPTMAVTTSALYGLGMGISVTAV
                     LLGSNIVISMVRKLIPDKIRIPAFITIIAGFTTIVQLLLKAYVPVLDKALGIFIPLIV
                     VNCIILARAEMFASQNTVFLSALDGIGMGFGYTLALFTIGSLREILGNGTWMGLRVTA
                     GLIDPAVMMILPPGGFIAFGLVIALANKFSKVKVEHNNCQSCGMCGKTAAAFADSGQV
                     GIDSKKDLKEAQAMRKDAQKDGREAITK"
     gene            complement(755..1375)
                     /locus_tag="PK733_11635"
     CDS             complement(755..1375)
                     /locus_tag="PK733_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOJ11230.1"
                     /translation="MENIFKPALVLFIICLVVTAALAFTYNTTKDTIDQRAVLDAENA
                     RKEVLSESESFKQLEDDEVIDITEKIPGTGIVKEVYEGIKDTGTAGTGTAGFVFNVTN
                     KGYGGEIDVIVGIDMTGKVTGVKIGKNTETPGLGSKATDKPFLSQLTGIVPKGPLKVI
                     KGESSGKDEEISAVSGATVSSRAIVEAVQAALNVYKELAKERGIAQ"
     gene            complement(1359..2330)
                     /locus_tag="PK733_11640"
     CDS             complement(1359..2330)
                     /locus_tag="PK733_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOJ11231.1"
                     /translation="MGNTLILSSSPHIRGRDTTQRIMIDVVIALVPAIISGVRFFGPR
                     VLAVLLTTVVSCVLWEYLSEKAMKRRITISDFSAVVTGVLLALNLPPSIPLWIAAVGG
                     FVAIVLIKQLFGGIGQNFMNPALGARVILMLSWTQQMTNWTNPLAPDAVSSATPLAVL
                     KKGHEIVDMVRPDYLQFFLGHAVGSIGETSVLALLIGGLYLIYRKVISPVIPLSFIGS
                     AALFGWIFGGNTPFTGDFLYHILAGGIILGAFFMATDYSTSPVTTKGRIIMGIGCGII
                     TSIIRLYANYPEGVSFAILLMNVLTPMIDKYTIPATFGGEGKVGKHI"
     gene            complement(2343..3650)
                     /gene="rsxC"
                     /locus_tag="PK733_11645"
     CDS             complement(2343..3650)
                     /gene="rsxC"
                     /locus_tag="PK733_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOJ11232.1"
                     /translation="MISKGVFKGGVAAPHRKNTAEFETIRMGVPEKVIIPMLQHIGAP
                     CKPIVKKGDIVKVGQMIGQSEKYVSAPVHSSVSGTVTNVGSVVHSGGMYIEAVEIKTD
                     GNQEIHESIKPPVYSSDSELLELIKQSGLVGLGGAGFPAHVKLAPQTDKNIDTLIVNG
                     AECEPYITSDYREILENSWNVICGINILMDLLKVNNVLIGIEDNKPEAIKVLTELAAT
                     SEKINVVKLKSRYPQGAEKMLIYSITGRKVPPGKLPLDVGVVVMNVNSVSFIAEYVKT
                     GMPLIKRRVTVDGPAVKNPSNVDVFIGTPIVDVFEFCGGLKTDVEKVLMGGPMMGVSQ
                     SSLDNPVLKHTNALLAFGPEFGKLPEESACIRCGKCVEACPMNLLPLYLNLNAIHENV
                     DGLRKYKAMDCIECGSCSYVCPAKRHLVQSIRLGKALLRKKKV"
     gene            complement(4090..5376)
                     /locus_tag="PK733_11650"
     CDS             complement(4090..5376)
                     /locus_tag="PK733_11650"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ11233.1"
                     /translation="MLKEINIAFLGFGTVGTGAYTIISSNGRKLFEKEGISINVKRIL
                     VRDVAKKRSVVVDKSLLTDNIDDIINDPEISIVAEFIGGEEPAREYILAALKSKKTVV
                     TANKEVMARHWEELEKTARSTGAGLYYEASVGGGIPIIKVIRESLQANNINEIMGIIN
                     GTTNYILTKMSEDGRSFESVLEEAQKLGYAEPDPTFDIEGLDAMFKLSILSTLAFHTR
                     IHIDKIYREGITRITTDDIAFGKELGFSIRLLAIAKKHGQNIEVRVHPTFIPADHPLA
                     SVRDAYNAVFIRGDAVGNLMLYGRGAGDLPTGSAIVSDIVNACLHESGHTYSTLSNGD
                     WTPDKILFNDNWETEFFIRMTVKDQPGVLAKIAGIFGKYGVSIASVIQKRRNGDSTPI
                     IFVTHKARELSIDKAIDEIKEESVVIRVENVIRVER"
     gene            complement(5656..6522)
                     /gene="prmC"
                     /locus_tag="PK733_11655"
     CDS             complement(5656..6522)
                     /gene="prmC"
                     /locus_tag="PK733_11655"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOJ11234.1"
                     /translation="MNIRSALSTGTKILKSVGNQSPAADAGVILCFLLNCDKTFLFSH
                     DDYELTEEEQETFKKLIGKRSEGVPLQHITGRREFMSLDFIVNSHVLIPRQETEMLVE
                     YVIKYINNLTYKYTIKPEMFNILDIGTGSGCIAVSLAHYVKNCGITAVDISEEALNTA
                     RLNAVKNGVEDRINFILSDLFNEVAGNLYDVIVSNPPYIAKKEIETLQVEVREHEPII
                     ALDGGVDGFDFYTRITEEAPLFLKVGGILAVEVGYNQAGRVADLMRVCNFRKIEIIKD
                     LDGIDRVVIGQI"
     gene            complement(6519..7406)
                     /locus_tag="PK733_11660"
     CDS             complement(6519..7406)
                     /locus_tag="PK733_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ11235.1"
                     /translation="MIGPQKAATAIRKSDGEIVVEKKNLPPKDIYSKIPFVRGVFNFV
                     RQMVFGMKALMYSAEFMDIEDDSLEPSKFDKFVERLLGDKFKEAAIYFSVFIAIILGI
                     SLFMLLPNFLADFFKGIDKTTRGGVIQRNLLEGIIRLVIFLCYLFLASRLNDIKRVWQ
                     YHGAEHKTIHCYENKEELTIENVQKYSTKHPRCGTSFLFIVMIVSIIVFSFAGWHNRW
                     INLLLRLLLIPVVAGISYELLKLAGNSKNKIVGLINYPGMALQLLTTKQPDDDQVEVA
                     IAALNSVLKEDEAGERHEN"
     gene            complement(7760..7969)
                     /gene="rpmE"
                     /locus_tag="PK733_11665"
     CDS             complement(7760..7969)
                     /gene="rpmE"
                     /locus_tag="PK733_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOJ11236.1"
                     /translation="MKEGIHPKYNEAVVKCACGETFTTGSTKKELRVEICSKCHPFFT
                     GKQKFIDTAGRVEKFNRKYGLGNKE"
     gene            complement(8136..10310)
                     /gene="rho"
                     /locus_tag="PK733_11670"
     CDS             complement(8136..10310)
                     /gene="rho"
                     /locus_tag="PK733_11670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOJ11237.1"
                     /translation="MNNVNLWDKKLEDLRFIAKVMGIKSVTKYRKNELIEMILEEGKV
                     SSGEEYIKPVLDGKNDIETYNDEKADINQDIVTPDENQYIETPEDKQDIENPKEKQNV
                     EKSNEIDEISETKISSGAEDINQVNIQNEIASPQEKQKSQEIQKEEGKAGTKRTRRTR
                     KSAEKIEQTDGADVAVVDSKPEEQKPKRTRRGRPPKAKEPAETSDITEDKDAKTTDIT
                     DDKSAAKIVPKAQDDNIEDTETERKAEEKQEKALIEEKYGKVLTEEDNKEKVIEDNKL
                     RDIKEGKKQIIETKEERRPDSPKSTATGTAPVAPDTEQVETKERPREETKRYEEKLSG
                     RLYEPRRDNWNNGLEKIESDDPVEGVLEVLPDGYGFLRSDNYLPGSKDIYVSPSQIRR
                     FGLKTGDKLKGKGRIPKEGEKFQALLYVQTVNGDPPDTASRRTPFEYLTPIYPNKKII
                     LETSPKELSTRLIDLVAPIGKGQRGMIVSPPKAGKTILLQKIANSITTNYPEIELIVL
                     LIDERPEEVTDMQRSIKGEVVYSTFDKVPEHHIKVAEMVLERAQRLVEHKKDVVILLD
                     SITRLARAYNLTIPPTGRTLSGGLDPGALHSPKRFFGSARNIEFGGSLTIIATALVDT
                     GSRMDDVIYEEFKGTGNMEIHLDRKLSEKRIFPAIDINKSGTRREELLLSQKELEGIW
                     AIRKAMSNMGTSDVTEMLINRLVQTKSNEEFVNSINLAFVER"
     gene            11136..>12479
                     /locus_tag="PK733_11675"
     CDS             11136..>12479
                     /locus_tag="PK733_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11238.1"
                     /translation="MEKFLLSLLAAVLVVSVFVVALFLRKKNERLRLIGIIKERWGKN
                     PLRYSKSTINENTHCYYSNMKDNNITTNTSNTTNTNNTTNANNNTNNNCNNDNCGVSL
                     HIDDITWNDLDMDNVFNRINNTGSTVGEQYLYYLLRNPVFDGSVLKERERLTAFFQSN
                     RKQRMDLQFLLAKLGKTKSIDITNYFLNEQKYASYESLKYKLLSIGFLLSPLLMIFNL
                     SFGIFVAIAFLTTNITVYYKKKYEIEAHLEAFNYIVSLVDCADKISNLNIKEISTYQN
                     SLKACLKKLKGVISKSFLVLYRTEDPFLEYVKVILLGEIIAYESMFKLILKYKQDLMD
                     IFNTVGLIDSLISVASYRVSIDYYSRPELYSIKNVLNNSNNNEHNSSDSSGSGSLSKS
                     VDFKDICHPLINNPVPNSLCIDKPILITGSNASGKSTFLKTIAINAIFAQTIYTCL"
BASE COUNT         3638 a         2653 c         1846 g         4342 t
ORIGIN      
        1 ttgtctcatt tggttattgc ctccctgcca tctttttgag cgtcttttct cattgcctgt
       61 gcttctttca ggtctttctt gctatctata ccaacctgcc cgctatcagc aaaagcggct
      121 gcagttttac cacacatacc gcatgactga caattattat gttcaacttt cactttcgag
      181 aatttattgg ctaaagcaat aacaagccca aatgcaataa agccgcccgg aggaagtatc
      241 atcataactg cggggtcaat aagacctgct gtcaccctta gccccatcca ggtgccattg
      301 ccaagtattt cacgcaatga accaattgta aacaatgcca gggtatatcc aaaccccatt
      361 cctatcccat ccagggccga tagaaaaact gtattctgac tggcaaacat ctcagccctt
      421 gccagtatta tacaattaac cactattaac ggaataaata ttccaagtgc tttatctaaa
      481 accggtacat atgctttaag taaaagctgc acaatagtcg taaaccctgc tattattgtt
      541 ataaaagcag gaatccttat cttgtccggg attaatttcc ttaccatgga tattactata
      601 ttagacccca atagaactgc agttacagaa atacccattc ccaagccata tagggccgat
      661 gtggttaccg ccatagtggg acacgtacca agtacaagcc ttaaactggg gttttcttta
      721 atcaaaccat ttgtcaatgt tttcattgct cttttcactg cgcaataccc ctttcttttg
      781 ccagttcctt atatacattt aatgcagcct gcacagcctc aacaatagct cttgatgaaa
      841 ccgtagcacc gcttaccgca cttatttcct cgtctttacc gctgctttcc ccttttataa
      901 ccttcagagg ccccttaggt acaattcctg tcagctgtga aaggaaaggt ttatctgttg
      961 ctttggaacc aagtccggga gtttcagtgt ttttaccaat tttaactccg gtaacttttc
     1021 cggtcatatc tatacctaca attacatcaa tttcgccgcc gtagccttta tttgtgacgt
     1081 taaaaacaaa tccagctgtc ccagttcccg ccgtccccgt atctttaatt ccttcatata
     1141 cctctttaac tatgccagtt cccgggattt tttcagtaat atctataact tcatcatctt
     1201 ccaactgctt aaagctctca ctttcagaga ggacttcctt tctggcattc tccgcatcca
     1261 ggacagccct ctgatctatt gtatccttgg tagtgttata agtgaacgca agggcagcag
     1321 ttacaactaa acaaataatg aataaaacca gggcaggttt aaatatgttt tccaacttta
     1381 ccttcacctc caaaagtcgc agggattgta tatttatcaa tcattggggt tagaacattc
     1441 attaacagta tggcaaagga aacaccttca ggataatttg catacagcct gattattgaa
     1501 gttattatac cgcagccgat gcccataata attcttcctt tagttgtaac cggggatgta
     1561 gaataatccg ttgccataaa aaatgcacct aatataattc cacctgccaa tatgtggtat
     1621 aaaaagtcac ctgtaaaagg tgtatttcct ccaaatatcc agccaaataa tgctgcactt
     1681 ccaataaaac ttaaaggtat taccggagaa ataactttcc tgtaaatcaa atacaaccca
     1741 cctattaaaa gtgccagcac tgaagtttct cctattgagc caacagcatg ccccagaaaa
     1801 aattggagat aatccgggcg caccatgtcg acaatttcat gacctttttt caagaccgcc
     1861 aggggggttg cagaagatac tgcatccggt gccagaggat tggtccagtt tgtcatttgc
     1921 tgtgtccagg aaagcattaa aataacacgg gctcccagag caggattcat aaagttctgg
     1981 cctattcccc caaataactg ctttattaaa acaatagcca caaatccacc tactgctgca
     2041 atccataaag gtattgaagg cggcaggttt agtgcaagta aaactccggt aaccactgcg
     2101 ctgaaatcgg aaattgtaat tctccttttc atggctttct cagataaata ctcccataaa
     2161 acacagctga ccacagtagt tagaagtacc gctaaaaccc tcggcccaaa aaaacgcact
     2221 cctgatatga tagcaggtac aagagcaatt acaacatcta tcattatcct ttgtgtagta
     2281 tccctgcctc ttatatgagg agaagaagac aaaatcaagg tatttcccaa aataaacccc
     2341 tcctaaactt tcttttttct aagcaaggcc ttgcccaatc ttatagactg cacaagatgt
     2401 ctcttagccg gacaaacata agagcagctt ccacattcga tgcagtccat agccttatat
     2461 ttccttaatc catcaacatt ctcatggata gcattaagat ttaaataaag tggtaaaaga
     2521 ttcataggac aggcttctac acactttccg catcttatgc aggcactctc ttcagggagc
     2581 tttccaaact cagggccaaa agccaaaaga gcattggtgt gtttaagtac agggttatca
     2641 agagagcttt gtgatactcc catcataggt ccgcccatta aaaccttttc aacatctgtt
     2701 tttaatccac cgcagaattc aaaaacatcc actattggag tacctataaa gacatccaca
     2761 ttagacgggt ttttaactgc cggaccatca acagtcactc tccttttaat aagaggcata
     2821 ccggttttta catattcggc tataaatgat acgctgttaa cattcattac aaccacgcct
     2881 acatctaatg gcaatttacc aggcgggact tttcttcctg ttattgagta aattagcatt
     2941 ttctctgctc cctgaggata gcgtgattta agtttcacta catttatttt ctcgctggta
     3001 gctgcaagtt cagttaacac tttaattgct tcaggcttat tatcttcaat acctatcaac
     3061 acattattaa ctttgagtag atccattaaa atatttattc cgcaaattac gttccaggag
     3121 ttctccaata tctcccgata gtcggaagta atatatggtt cacactcagc tccatttaca
     3181 attaaagtat caatattttt atctgtttgt ggggcaagtt tgacatgagc cgggaagcct
     3241 gcaccgccaa gacctacaag acctgattgt ttaattagtt ccaacaattc tgaatcactt
     3301 gaataaacag ggggttttat gctttcatga atttcctggt tgccatcagt tttaatctcg
     3361 acagcttcta tatacatacc tcctgaatga acaacggaac ctacatttgt tacagttccg
     3421 gatacacttg aatggactgg agcagaaaca tatttttcag actgtcctat catttgccct
     3481 acttttacta tatctccctt tttaactatg ggcttacagg gtgcacctat atgttgaagc
     3541 ataggtatta ttaccttttc aggtaccccc attctgatag tttcaaattc agcagtgttt
     3601 tttctatgag gtgctgccac tcctccttta aatacccctt ttgaaattat cattttactt
     3661 gaccccattc ataagctatt tagtacaacc ggccaactat gcaactgatt aacttgctat
     3721 atagatgacc tactattggt ttttctatta aatacattaa tatatacggt attttctttt
     3781 ttactatgaa actatggtta tagcaatcct cttaattata attattacca ggtaataagt
     3841 caaataattg gcacttagga tttaaaggct ttatatcatt tatttaattt tatcttcatg
     3901 ttaatatgac atacttcata tattacagtt tacttgcagt tttgtatata ttatttaaca
     3961 tcatcccatt gcacctcttg ccagtatagg attccgggta ttggctatga acattgtata
     4021 caacattggt gatgctttta gagcatcatc atcccaatct atatctctat tcagagtgca
     4081 tttatatctt taccgctcaa cccttataac attttcaacc cttataacaa cagattcttc
     4141 ttttatctca tcaattgcct tatctattga aagctctctg gctttatggg ttacaaatat
     4201 gattggagtg ctatcaccat tacgcctttt ttgtataact gatgcaatac tcactccgta
     4261 ttttccaaaa ataccagcaa tttttgcaag cacacccggt tgatctttta ctgtcattct
     4321 tatgaagaac tctgtttccc aattatcatt aaataaaatt ttgtcaggtg tccaatctcc
     4381 attggataga gtggaataag tatgcccgga ttcatgcagg catgcattaa caatatccga
     4441 cacaattgcg cttccagtag gaaggtctcc tgctcccctg ccatacagca ttagattgcc
     4501 gactgcatca cctcttataa acacagcatt atatgcatca cgtactgacg ccaatgggtg
     4561 atccgccgga ataaatgtag gatgaaccct gacttcaatg ttttgcccgt gttttttcgc
     4621 tatagccaaa agccggattg aaaaacccag ttcttttcca aatgctatat cgtcggtagt
     4681 tattcttgtt atgccttctc tatatatttt atcaatatga attcttgtat gaaaagcaag
     4741 agtcgataaa attgagagtt taaacattgc atcaaggcct tcaatatcaa aagttgggtc
     4801 tggctctgca tatcctagct tttgagcttc ttccaataca ctttcaaagc ttcttccatc
     4861 ttcagacatt ttcgttaata tataatttgt ggtaccattt attattccca taatttcatt
     4921 tatgttattt gcctgcagag attcccttat aaccttaatt atgggaattc caccgccaac
     4981 acttgcctca taatacaagc ctgcaccagt tgatcttgca gttttttcca attcttccca
     5041 atgccttgcc attacttcct tatttgcagt aaccacggtt tttttgcttt tcagtgctgc
     5101 caggatatat tctctggctg gttcctcacc acctatgaat tctgcaacta ttgaaatttc
     5161 agggtcgttg attatatcat caatattatc cgttagcaaa ctcttgtcta caactacact
     5221 tcttttttta gcaacatctc tgacaagaat tctcttaaca ttaatgctta ttccttcttt
     5281 ttcaaataac tttctgccat tactgctaat tatcgtatat gcacctgtcc ctacagttcc
     5341 aaagcctaaa aaagctatat taatttcttt tagcattata tcctccaagt acaagtattc
     5401 catcaataaa atttattctg caagacacca tttttcaaat tgacaaaatt tattacatat
     5461 tataacgcat cagcagaaaa tttttcggtt gtagaaaaac tttctaacct tcgcgcattt
     5521 caacgaggca ggagacatct ctcacctcgt tatattgcat aattatatat tattttacag
     5581 caatttatca agtataagtt ttctataggg gcgcattttc ttcttatgat acaaaatata
     5641 gaagatgcgc cctttctata tttgccctat tacaacccta tcaatcccat ccaaatcttt
     5701 aataatttct attttcctaa aattacatac cctcattaaa tcagcaactc tgcctgcctg
     5761 gttgtatccc acttcaacag caagaattcc gccaaccttc agaaataaag gtgcttcttc
     5821 tgtaattctt gtataaaaat caaatccgtc cactccgcca tccagagcta ttattggttc
     5881 atgctctctt acctcaacct gaagtgtttc aatttctttt ttagcaatgt atggagggtt
     5941 tgaaacaatt acatcataaa gattccctgc cacctcgttg aataaatcgc tcaagataaa
     6001 attaattctg tcttccacac cgttttttac tgcatttagg cgagctgtat ttaatgcttc
     6061 ttcagatata tctacagctg ttattccgca atttttaaca tagtgtgcaa gacttacggc
     6121 tatgcagcct gagcctgttc ctatatcaag aatattaaac atttcaggtt tgatagtata
     6181 tttatatgtt aaattattaa tatatttaat aacatattct acaagcattt cagtttcctg
     6241 acgtggtata aggacatggg aatttactat aaaatccagg gacataaatt ctctccgccc
     6301 tgttatgtgc tgcagaggca ccccttcaga tcttttccct atgagtttct tgaatgtctc
     6361 ctgttcttct tcagttaatt cataatcatc atgagaaaat aaaaaggtct tatcacaatt
     6421 taagagaaaa catagaatca ccccggcatc agcggccggg gattgatttc caacagattt
     6481 taatattttc gttcctgtac ttaatgcact tctaatgttc aattctcatg cctctctccg
     6541 gcttcatctt ccttcaatac gctatttaat gcagctatag ccacttcaac ctgatcatca
     6601 tcgggttgtt tggtggtcaa aagctggaga gccattcccg gataattaat taaacccact
     6661 attttatttt tgctattccc tgccaattta agcagttcat atgatattcc tgctaccaca
     6721 ggtatcaata acagtctcaa cagcaaattt atccatctat tatgccaccc tgcaaatgaa
     6781 aatacaataa tacttacaat catcactata aacaaaaaag atgtaccgca tctagggtgt
     6841 tttgttgaat atttctgcac attttcaata gtaagttctt ctttgttttc gtagcaatgg
     6901 atggttttat gctctgcacc atgatactgc caaactcttt taatatcatt aagtctggaa
     6961 gccaggaaaa gataacataa gaatataaca agccttataa taccttctaa taaatttctc
     7021 tgtattacac cgccccgagt agtcttatct atccccttga aaaaatctgc cagaaaattt
     7081 ggcaagagca tgaaaagact tataccaaga attatcgcaa tgaatacaga aaaataaata
     7141 gctgcttctt taaatttatc ccccaacagc cgctctacaa acttatcaaa ctttgatggt
     7201 tccaggctat catcttcaat gtccataaat tccgcagaat acatgagagc tttcatccca
     7261 aataccattt gccttacaaa gttgaacacc cctcttacaa aaggaatttt tgaataaata
     7321 tctttcggag gaagattctt tttttctaca acaatttctc cgtccgactt tcttattgca
     7381 gttgccgcct tttgcggccc tatcattaag agtccttcaa taagtgcctg tccccctaca
     7441 ttgcaataat tatttttact actttccctt cttttatttg aatttttatt tatattatta
     7501 ttgcagtttt ctacgtagct atcagattta ttcttgtcca tgttaacacc tcactctgat
     7561 tatatcagat atttcaagct aatgtaaata cacgggtgca tttaatttat atgaagaaaa
     7621 tattttataa atgcacccaa aaatgcagaa atatatatta cccccatata cgaacaaagg
     7681 ctgagacata ttgtaagtcc cagcccttat atttagctca ctgtatatag ttcactgtat
     7741 ttagcttact ctatatagct tactctttat ttccaagtcc gtatttccta ttaaacttct
     7801 caactctacc tgcagtgtct ataaatttct gtttccctgt aaaaaacgga tgacactttg
     7861 agcaaatttc aacacgcagt tccttttttg tagaacctgt agtaaaagtt tcaccacatg
     7921 cacatttcac tactgcttca ttatactttg gatgtattcc ttccttcata ctttcacctt
     7981 ctttcgagat acttttatac taaaactcta tgcgattaaa gtcgttaagc attctgtaaa
     8041 taattattgt taatattcat aagtttaatc tatacaacaa aacacatttt aacataaatt
     8101 gatcatatag tgcaagtata ttttttaaaa aagttttacc tctctacaaa agccaaattt
     8161 atgctgttta cgaattcctc attgctcttt gtctgtacaa gcctgttaat tagcatttca
     8221 gtaacatctg aagtacccat attgctcata gcttttctta tagcccagat accttccagt
     8281 tccttctggc ttaagagcag ttcttctctc ctggttccag acttattaat atcaatagca
     8341 gggaatatcc tcttttcaga aagcttcctg tcaagatgta tttccatgtt tccagtcccc
     8401 ttaaactcct catatataac atcatccatc ctgctgcctg tatcaacaag tgcagttgca
     8461 attattgtaa ggcttccgcc aaactcaata tttcttgctg aaccaaaaaa tcttttgggg
     8521 ctatgaaggg ctcccggatc aagaccacct gacagagttc tgcctgttgg aggaatggta
     8581 aggttgtatg ctcttgccag ccttgttata ctgtccagca aaattactac gtccttctta
     8641 tgctctacaa gcctttgcgc tctctcaagc accatctcag ctactttaat gtgatgttct
     8701 gggaccttgt cgaaggttga atatacaact tcgcctttaa tcgaacgctg catatccgtt
     8761 acttcttccg gcctttcatc tattaataaa acaataagct caatttccgg ataattcgta
     8821 gttatgctat tggctatttt ctgcagaaga attgttttac cagctttagg aggtgaaact
     8881 atcattcctc tctgaccttt acctatagga gctacaagat ctattagcct tgtagacaat
     8941 tcctttggtg atgtttcaag tatgattttt ttgtttgggt aaataggcgt aagatattca
     9001 aatggtgttc tccttgaagc agtatcaggt ggatctccgt taacagtctg tacatataac
     9061 agtgcttgga acttctcccc ttctttaggt atcctgccct taccttttag tttgtctcct
     9121 gttttcagtc caaaacgtct tatttgtgaa ggtgatacat aaatatcctt ggagccggga
     9181 agatagttat cactcctaag aaacccgtag ccatcaggca atacttccaa tactccttct
     9241 acaggatcat cgctctcaat cttttcaagg ccattgttcc agttatctct tcttggttca
     9301 taaagtctcc ctgaaagctt ctcttcatat cttttagttt cttctctggg acgttccttt
     9361 gtttccacct gttcagtgtc tggtgcaaca ggtgctgtgc cggttgctgt actcttaggc
     9421 gaatcaggtc ttctctcttc tttagtttct attatttgct ttttaccttc ttttatatct
     9481 ctaagtttat tatcttcaat aaccttttcc ttattatctt cttctgtaag tactttacca
     9541 tatttctctt ctattaaagc tttttcctgt ttttcttcag cttttctttc agtttcagta
     9601 tcctctatat tatcgtcctg agcttttgga acaatttttg cagcagattt atcatctgta
     9661 atgtcggtag ttttagcatc cttatcctct gtaatatcag aggtttctgc aggctcctta
     9721 gctttaggcg gtcttcccct tcttgtcctt tttggttttt gctcttccgg tttggaatcc
     9781 acaacagcaa cgtctgctcc gtcagtctgc tctatttttt cagcactctt acgagttctt
     9841 cttgttcttt ttgttccagc ttttccctct tctttctgaa tttcttgaga tttttgtttt
     9901 tcctgggggg atgcaatttc gttttgaata ttgacttgat ttatatcttc agctccacta
     9961 gaaattttcg tttctgatat ttcatctatt tcgttactct tttcaacatt ctgtttctct
    10021 ttcggatttt caatatcctg cttatcttca ggggtttcta tatattgatt ttcatctgga
    10081 gttacaatat cttgatttat atccgctttt tcatcattat atgtctctat gtcgtttttc
    10141 ccatctaata caggcttaat atattcttct ccagatgata cttttccttc ttcaagtatc
    10201 atttctataa gttcattttt cctgtattta gtaacgctct taatccccat tactttagct
    10261 ataaatcgca aatcttcaag ttttttatcc cataagttta cgttgttaat gctttccata
    10321 atacaccacc ttatctatat aatcactatt caatatttta atcatatggt attctcgcat
    10381 tcccgacatt tttatataaa gttgaccggt tcagaatcca ccttttttga ttattcgctt
    10441 taattataca aattgaaatt tattgtcgat tcatctcaca acccaattcg cgagtattat
    10501 caacaaacag cactataaaa actaaataat taaaaaaaaa taaataaaaa tatatgtcct
    10561 ataagttcta tacgtccttt acaaattata ttaagcttcg aaataaggaa tagagaagat
    10621 ttatgaactc tatttaatta attatagcat tttaaaaact tccatgtcaa cacttttttc
    10681 cgcctttttc cgattttttg ctgttttacg ttattataat taccatgaca aacagattta
    10741 tgatataatt ctaaaggtat tcagttttgg gtaattgtat actgctttgt aatgttgcca
    10801 atggaaagcg catttctgga gggtaatctt tgccgacccg taaggaatgc atatgaggga
    10861 aggatcataa ggccttttat agggacattt ccccatacga catcttcccg atgcaataaa
    10921 aattatcctg gaagacatca ctttccaaat tggcatagta atatatattt aatgttttag
    10981 ttatagtatt aatagtatta atactattaa atatattatt accacttttt ccggtgttaa
    11041 actctaataa tctgaaattt gcagaattta ttagtacatc tgaatcaaat actatacctg
    11101 aacaaatact aaaatttatt tatataggtg atgctatgga aaaattttta ctatctttac
    11161 ttgctgctgt tttagtggtt tctgtttttg ttgttgcatt gtttttaaga aagaaaaatg
    11221 aacgtttaag attaattgga attattaaag aaagatgggg caagaatccc ttgcgttatt
    11281 ccaaatccac aattaatgaa aatacgcatt gctattatag taatatgaaa gataataata
    11341 ttactaccaa tacttctaat actactaata ctaataatac tactaatgcc aataataata
    11401 ctaataacaa ctgtaataat gacaattgcg gggttagtct gcatatcgac gatattacct
    11461 ggaacgatct tgatatggat aatgttttta atagaattaa taataccggt tccaccgtag
    11521 gcgagcaata tctttattat ctcctcagga atcctgtttt tgatggttca gtgctcaaag
    11581 aaagggaaag actcactgct ttttttcaaa gcaatcggaa gcaaaggatg gacttacagt
    11641 ttttattagc aaaactgggt aaaactaaaa gtattgatat tacaaattat ttcttgaatg
    11701 aacagaaata tgcttcatat gaaagcctta aatataagct cctctcaatt ggttttttat
    11761 tatcaccttt attaatgata tttaatttat cttttggtat ttttgtggca attgcatttc
    11821 ttactacaaa cataactgta tattataaga aaaaatatga gattgaagct catcttgaag
    11881 cattcaatta tattgtcagt ctcgtggatt gtgctgacaa aatatcaaat cttaatatta
    11941 aggaaattag tacctaccag aattcactaa aagcctgttt aaaaaaactc aaaggtgtaa
    12001 taagcaagtc atttctagta ttgtatagga cagaagatcc ttttcttgaa tatgtgaagg
    12061 ttattttgct tggagaaatt atagcttatg aaagtatgtt caagcttatt ctcaagtaca
    12121 agcaggatct aatggacatt ttcaatactg taggattgat tgacagcctt atatcagttg
    12181 catcatatag agttagtatt gattattatt caagacctga gctatactca attaaaaatg
    12241 ttctaaacaa ttcaaataat aatgaacata atagttcaga tagtagtgga agcggcagtt
    12301 taagtaaaag tgttgatttt aaagatatct gccatcctct tattaataac ccggtaccta
    12361 attccctttg catagacaag cctatactga ttaccggctc taatgcttca gggaagtcaa
    12421 catttctgaa aactatagct attaacgcca tcttcgctca aacaatatac acatgtctt
//
LOCUS       DAOSDD010000127        12450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1958_length_12450_cov_5.568294, whole genome shotgun sequence.
ACCESSION   DAOSDD010000127 DAOSDD010000000
VERSION     DAOSDD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_12450_cov_5.568294"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   8..760
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8..37
                     /rpt_unit_seq="gtagaaccgatacatatgatgtattgaaat"
     gene            2896..4578
                     /locus_tag="PK733_11680"
     CDS             2896..4578
                     /locus_tag="PK733_11680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ11239.1"
                     /translation="MTYNPNNPLVVQGDRTILVEVNHPMYEDVRDEISAFAELEKSPE
                     FVHTYRISPLSLWNAASQGMKANSIIDVLKRYNKYDLPQNVQRDIEDYVGRYGKVQLL
                     NENGQLVLVSEDPYIIKEIAHSKDIKIYLKGQVNASKLLVDPSMRGHLKYALTRIGFP
                     VEDVAGYMEGTSFDIYLKDNTNEGKKLQLRDYQKQAVSSFYDGGSKKGGSGVIVLPCG
                     AGKTIVGMAAMEKIKSETLILTTNITALRQWKEELMDKTSMTSENIGEYSGENKEIKP
                     VTIATYQILTHRNDKNDNFPHMELFNKKNWGLIIYDEVHLLPAPVFRVTAELQAKRRL
                     GLTATLVREDGLEEDVFSLIGPKKYDMPWKVLEKQGWIACANCTEIRVPMPESIKMDY
                     AISESRTKFRIASENYKKIDIIDKLLEKHSEDNVLIIGQYIDQLNEIAKKIGVPVITG
                     KTPNHERMALYDNFKKGKIKRLIVSKVANFAIDLPDANVAIQVSGTFGSRQEEAQRLG
                     RILRPKTGENIAFFYSVVTKDSREQDFAVNRQLFLTEQGYKYYIKTAEEIEE"
     gene            4587..6317
                     /locus_tag="PK733_11685"
     CDS             4587..6317
                     /locus_tag="PK733_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOJ11240.1"
                     /translation="MTLKVYLENMNVTDYGNNSLSKLIKIAGLKGVSTRKAAMVSALD
                     TFLSDIWNIQQTWKALNKYEKEILEEYIRSGRTLDSGDMKAIMEKYGKGVKFFSATKL
                     VDCFSENSPAKLFFLPTHMPEQIYNILKDLVKPLEIRFNPVTEISKENVSYELFIGEQ
                     FYQDFVQMIRLVNSNKLKTTKGSKFPTKASTVKINEVLINKEILTDSYIDIENIRTIE
                     QTTRIYGIIQLMFDSGLLEEKNGILALGPEAEKFLKLNYIDKSKELLHTYIDSKNINE
                     IERISEIKVRTYRQPYFKTSRETILRYLSMCPIDQWVERSEFLKVIKRNSRKFILDET
                     GNIESYDDYYRYYTDSDLGFNEIEGRFIDVMLIEYLSALGIVDVMLDEWSSEFYNYSY
                     FVSSYFKLTPMGAYILGVNEGYISDKEIDSAGFGLIVQPNYEILIPEGSMKDLHVIFF
                     DRFAEKISEDKVNIYKLSFKSIITALDNGIGVGEIIEYIKKYCQHDIPENVLITLNEW
                     EQESKRIRIRTVTIVEADSKYLIEELKAYKSISGNIKKELPFIFEIENNASNKVKREI
                     EKKNHFCLLE"
     gene            6755..7579
                     /locus_tag="PK733_11690"
     CDS             6755..7579
                     /locus_tag="PK733_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ11241.1"
                     /translation="MKLTFNGTSAAEGFPALFCECRHCQKARELGGKNVRTRTSCLID
                     DQYLIDFPPDTYMHVLHGKLKLSKIKHIIVTHSHEDHFQPYELLMRRPPFAYPGEDEI
                     LYVYGNDGVYEKFVQAGCTNENNKKYLQYVHVYPGKQFKAGEANVLPLRADHTNGNEI
                     CHIYIINIGEKHLLYGHDTGYFPEETWEALRSIKLDGVILDCTCGTIECERGHMGITA
                     NVKVKERLIEQGSADEKTTFIVTHFSHNCGPLYDDMVKLAGENGFIATFDGMEINI"
     gene            complement(7865..8164)
                     /locus_tag="PK733_11695"
     CDS             complement(7865..8164)
                     /locus_tag="PK733_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11242.1"
                     /translation="MSNFSYKLKAIDTPAEGSPGTKVSLKVSIEEATEEISRVYLSVA
                     MYGIFEVLKKESDTSFSLNYYIPYDAPYGNYDISIWAVNSGNVKGPVTNLSFRVS"
     gene            complement(8293..8862)
                     /locus_tag="PK733_11700"
     CDS             complement(8293..8862)
                     /locus_tag="PK733_11700"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ11243.1"
                     /translation="MRNEIINAAGSIFSRKGFYKASMDEIAVEAGVAKGTLYYYFKNK
                     SELFRTVVVEGIDYLVKDLISLANSSDDSIENIIKSMIKTNIDYYLDYYELANIVLNE
                     ITSGIDDDVLKEIKNSKDKYIEFLAELLSNGYKQGIICYSDFNLLAAGIIGMINGICK
                     YYLMNSSSVDRNSITEHACNMIMKGLFPK"
     gene            9189..9253
                     /locus_tag="PK733_11705"
                     /pseudo
     CDS             9189..9253
                     /locus_tag="PK733_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            9396..10076
                     /locus_tag="PK733_11710"
     CDS             9396..10076
                     /locus_tag="PK733_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOJ11244.1"
                     /translation="MNYKIFGDNMPAVSIRLNRGESIFTQSGGMVWMDSGITMITNMQ
                     GGFMKGLGRMFSGESLFMATYTSNAPNQEIVIASTFPGHIVDIDVGRTPIIAQRGAFL
                     CAQPSVSINAYVVKNMGAGFFGGEGFVLQRITGTGMVFLEVDGSLVERTLANGETIKV
                     DTGNVAAFEESVQYQVEMVKGFKNVLFGGEGLFLTTLRGPGKVWLQTMTMPSFAKQII
                     PFLPKSSN"
     gene            complement(10292..11413)
                     /locus_tag="PK733_11715"
     CDS             complement(10292..11413)
                     /locus_tag="PK733_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOJ11245.1"
                     /translation="MPPLSLLIKPASSSCNLRCKYCFYHSIAENRSIASYGMMNIETA
                     ELLIRKSFDYAENMCTFAFQGGEPTLAGLEFYKSFVELVNKYNTKKIGVHFAIQTNGT
                     LIDEQWADFLFKNNFLVGLSLDGPKEINDVNRVNHTLDGTFSKIMNTVNIFNKYGVQY
                     NILSVVNNSVARHTNKVYNFFKKYNFKYLQFIQCLDPLDEKPGGHSYSLTPLMYSNFL
                     KGLFDLWYNDITKGEIVSIRYFDNLVGMMMGHPPETCGMMGRCQCYFVIEADGGVYPC
                     DFYVTDQWKLGNIKENDFHELLNTDTGKRFIEVSMHVDDKCKECKWFNFCRGGCRRTR
                     EPFSDGLPVLNYYCESYYNFFEYAGERLYKLSRMFSRQV"
     gene            complement(11585..12235)
                     /locus_tag="PK733_11720"
     CDS             complement(11585..12235)
                     /locus_tag="PK733_11720"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOJ11246.1"
                     /translation="MKKTYTVKKIELNVTPIEKAWDSIEEINIDCFLWEDNGYKPKTM
                     AKLFYTDTHFHILFKSFEKEIKVKYFNMDEDVYKDSCVEFFINPNPDKDDRYFNFEMN
                     AAGTLLLGLGINRHGRKRFVIDNFREFFNIKTSVTKENLANYNGEFWTAQYSIPFTFL
                     EEHFGKLNFKPGCRLEANLYKCGDETGFPHYGCWNPITIITPDFHRSEFFGNLVLE"
BASE COUNT         4206 a         2074 c         2272 g         3898 t
ORIGIN      
        1 ttttgaggta gaaccgatac atatgatgta ttgaaatatt ggtgatacac ttggctttaa
       61 ccaaaacact tttagtagaa ccgatacata tgatgtattg aaatactaac agaagcgggt
      121 gtgcctgcat ggcagatgcg tagaaccgat acatatgatg tattgaaatc accctttata
      181 attaaagtgt atttcttgcc cactagtaga accgatacat atgatgtatt gaaattttta
      241 catttgtaaa ctatccccgg gtacatgtat atgtagaaac gatacatatg atgtattgaa
      301 atttgctttg ttaacggaag gaatccagcc taatatcgta gaaccgatac atatgatgta
      361 ttgaaataag ttgggcagta ggacaatttg cagacaaggt ccgtagaacc gatacatatg
      421 atgtattgaa attattgttt tgtttgtctt agttcttttg cttgttgtgt agaaccgata
      481 catatgatgt attgaaattt taaatgtctt attatccttt caggaatgtt ttctgtagaa
      541 ccgatacata tgatgtattg aaatctgtcc tgccccctta attctccagt ccggacctcg
      601 tagaaccgat acatatgatg tattgaaata ttttgcaggc tgctgttgaa tggcaagtca
      661 ttctggtaga accgatacat atgatgtatt gaaatttgcg gcttgatatg gaaaaattaa
      721 aggaagcttc gtagaaccga tacatatgat gtattgaaat aaatatagaa aatgtcaagt
      781 caacacctat ggattatgca aagtcaagaa ctccaatgtt tgatgataaa tgctatcctt
      841 taatacatac aataaggctt tggaatacca agacgcgtaa accagttgtg ttacagagct
      901 atgattgatg cctgtattga taagaaacct attatcacag cagatgctat caaaaagcta
      961 acagaaaaat atcctcatat ttttgataaa attgttcttt ccgaaagcag tacaaacctg
     1021 gtgcaaaatt aattatgaaa gtacaaaaat ggaggtgatt ttactttgga tatattaaga
     1081 aaccctacct ctatcaagct ccttgcagca tagataatta aagcttgtga cgagtatata
     1141 gctctacaaa ttatggaaaa acaattgaaa gaacttattt atcactatgc atcacaccac
     1201 ggcaataagt tattttctat cagaggtatt aatccgacta ttgtcaaccg tatagggaaa
     1261 aagaggttgg aacttctaaa cattacgctt gaaggatttc agcagaggct tttttaattg
     1321 tgcgtatgtc ctgttttata ttatactcta ttagtctgat cttaaggcct ggctctgtgt
     1381 agatagagtg ctctgcagga atgcaacact taaactccac aaccaggcta aaggatatta
     1441 tcctttcagg atgcagtaaa aaattctata taaatttcta tatatcagtg aaataatata
     1501 attttatttt atggtcttat tatttacaat gcaacctgcc cctgaagttt atcctgttca
     1561 aaccactgta gattagtttg tctcacaaca gtataacgaa gaatttcagc caactcatga
     1621 gtaaaatagt acgagccagt agtatagtgg aatgtataaa tagcctcata aggccatatc
     1681 ttttccttaa acgggtagtg ggtaataaat tttcagatct actccaatgt tactttaata
     1741 ctagggagta taaacgcagc tgtgtaccag agcgtaaggg taaaagctcg aaagagttgt
     1801 tgactggtaa gggctatccc tacttccttg aaattcttgg ctataagaga tgatattttg
     1861 aaccaatatt taggaaaagc ttattgaaat tacacattat ataatgttat attatggacg
     1921 tgtaatttcc taggaactgg tatgcttatt tttaaaaatt gtaaagtgaa tagacctgtt
     1981 tttgaaacac gccgaagatg tggtaccatg ctgcccgaac gattctactc ttccaatgaa
     2041 gcccgaaaca agtgactcgc atcttcgttg cggtatgcct acattaaagt tcgtctgtcc
     2101 caagatgtcg tgggaaaaat gcgaaaatgg aaagtacaga caccatactt cttgcgaaaa
     2161 cctctgcacg cattcacctt gcggttgtat gtctatccgg aaaaaatctt cgcaccttcc
     2221 ccggaaccgt tcgtggaact gaagaaagag acaataccta caaaatacat gtgaccttcg
     2281 agaaatccat caacccactt taaagacagt ttctgcattg cagggcaaaa aacaagaaat
     2341 gctcttacct cggaagctga tttgtattta gcaggcatta ctcagctagt tactgtgctt
     2401 cttactgata gcatacacca gcataaatac atacgatgcc ccaaaccgct gatttcagct
     2461 tagctgtact aaattctgta attcaaactt cctttcataa taatcctgct tttcataatg
     2521 caggtttatt tctacatgca ttcatgttga attaggatag ttatccacag cttttgtttg
     2581 tcgttattct cttttcaatg ttcactgctg cgcttgtgtc cgcagcattt ctagaatctg
     2641 gtatatgtgg ttctcttttt taagagtctc gcaattacct aaatatttga atgagcgtta
     2701 ttcaattata taacttttta tatatgttat ctatttatat cagcagcttg catttttgca
     2761 gaatattaat tttatcagct atcaattata attattgcct atggcatata aagtatatat
     2821 aatataaata ataaaaaagg atgctatatc catccgggat acttagcata aacactggaa
     2881 aggtagtgac attttttgac ttacaaccct aataaccctt tggttgttca aggggataga
     2941 actatattag tagaagtgaa ccatcccatg tatgaagatg taagggacga aatatccgcc
     3001 tttgcagaat tagaaaagag tcctgaattt gtacatactt atcgtatatc tccattatct
     3061 ctctggaatg cagcttccca aggcatgaaa gcaaattcca ttattgatgt tcttaaaaga
     3121 tataataaat atgatttgcc tcaaaatgtt caaagggaca ttgaagacta tgtaggccga
     3181 tatgggaagg tacaacttct gaatgaaaat ggtcaattag ttctggtatc tgaggatccc
     3241 tatatcatta aagaaattgc ccatagcaag gatattaaga tttatttaaa gggacaagtt
     3301 aatgcttcaa agcttctggt cgacccttcc atgagaggac atctaaaata tgcattaacg
     3361 agaattggat ttcctgtaga agatgttgcc ggttatatgg aaggaacatc ttttgatata
     3421 tatttaaagg ataatactaa tgaagggaaa aaacttcaac ttagagatta tcagaaacaa
     3481 gctgtgagct cgttttatga tggtggttct aaaaaaggcg gaagtggtgt aattgttctt
     3541 ccatgtggtg caggcaagac aattgtggga atggctgcca tggaaaagat caaaagtgag
     3601 acattgattc ttacaacaaa tattactgct ttaaggcaat ggaaagagga acttatggat
     3661 aaaaccagta tgaccagtga aaatatcggt gaatacagcg gggagaataa agagataaaa
     3721 cctgtaacta tagccacata tcagatatta acccatcgga atgataaaaa tgataatttc
     3781 ccccacatgg agctgtttaa caaaaagaat tggggattaa taatatatga tgaagtacac
     3841 ctgctacccg cacctgtatt cagggtgaca gcagaattac aagcaaaaag aagattaggg
     3901 cttactgcaa cgcttgtgag agaggatgga ctagaagaag atgtttttag tctgattgga
     3961 cctaaaaaat atgatatgcc atggaaggtg ctcgaaaaac aagggtggat agcatgtgct
     4021 aactgcactg aaataagggt accaatgccg gaaagtataa aaatggacta tgcaataagc
     4081 gaaagccgta caaagtttag aattgcctct gaaaattata aaaaaattga tataattgac
     4141 aagctgctgg aaaagcatag cgaagataat gtgctaataa ttgggcaata tattgatcaa
     4201 ttaaatgaaa tagctaaaaa aatcggggtt cctgtaatta ctggaaaaac accaaatcat
     4261 gagaggatgg ctttgtatga caattttaaa aaaggtaaaa taaagagatt gattgtttca
     4321 aaagttgcaa actttgcaat agatttacca gacgcaaatg tggcgataca agtatccgga
     4381 acctttggct cacgacaaga ggaagcccag cgcttaggca gaatcctaag gcctaaaaca
     4441 ggagaaaata tagcattttt ttatagtgta gtgactaagg atagtagaga gcaggatttt
     4501 gcagtgaaca gacaattatt tttaacagaa cagggatata agtattatat aaaaacagca
     4561 gaagagattg aggagtgata cggggaatga ctttaaaggt ttatcttgaa aatatgaatg
     4621 ttacggatta tggaaataat tcgctctcga aacttattaa aatagcaggt cttaagggag
     4681 tttcaactag aaaggcggct atggtttctg ccctggacac gtttttaagt gatatatgga
     4741 acatacagca aacctggaaa gcactaaata agtatgaaaa ggaaatccta gaagagtata
     4801 taaggagtgg aagaactctc gacagcggag atatgaaagc aataatggaa aaatacggca
     4861 aaggggtaaa gtttttttct gcaactaaat tagttgattg ttttagtgaa aactctcctg
     4921 caaaattgtt ttttcttccc acacatatgc cggaacagat atacaacatc cttaaagatc
     4981 ttgttaaacc tttagaaatc agatttaacc ctgttacaga gatatctaag gaaaatgtct
     5041 cttatgagct ttttattgga gaacaatttt accaggattt tgtacaaatg ataaggcttg
     5101 ttaacagtaa caaattaaaa acaacaaaag gcagcaaatt tcccactaag gcatccactg
     5161 taaaaatcaa tgaagtgtta ataaacaaag aaatccttac agatagttat atagacattg
     5221 agaatatccg tactatagag caaacaacca gaatatacgg aataatacag ttgatgtttg
     5281 actccggcct gcttgaagag aaaaatggga ttttggcttt aggcccggaa gctgaaaaat
     5341 tcctgaaact taattatatt gataaaagca aagaacttct tcatacgtat atagattcaa
     5401 aaaacataaa cgaaatcgaa agaatcagcg aaatcaaagt acgcacatac aggcagccct
     5461 attttaaaac aagtagagaa actattttaa gatatctttc tatgtgtcct atagaccaat
     5521 gggttgaaag atctgaattt ctgaaagtga ttaaaagaaa tagcagaaaa tttattctgg
     5581 atgaaacggg aaatatagaa tcttatgatg attattacag gtattataca gatagtgatc
     5641 ttggtttcaa cgaaattgag ggacggttta tagatgtaat gcttattgaa tatctttcag
     5701 cattggggat tgtagatgtt atgcttgatg aatggtccag cgaattctat aattattctt
     5761 attttgtatc ttcatatttc aaactaaccc caatgggcgc atatatcctt ggagttaatg
     5821 agggatatat ttcggataaa gaaatagaca gtgcaggatt tggtttgata gtacaaccta
     5881 attatgagat attgattcct gagggtagca tgaaagatct gcatgtcatc ttttttgaca
     5941 gatttgctga gaaaatttcc gaagacaaag ttaatatata caaactttca tttaaatcaa
     6001 ttatcactgc acttgataat ggtatcgggg ttggagagat tattgaatat attaagaaat
     6061 attgtcagca tgatattccg gaaaatgtcc ttattacttt gaatgaatgg gagcaggaaa
     6121 gtaagagaat tagaataagg acagtaacta tcgttgaagc tgatagtaaa tatctgattg
     6181 aggagctaaa ggcttataaa tctataagtg gtaatattaa aaaagaatta ccttttatat
     6241 tcgaaataga aaacaatgca tccaataagg ttaagcgtga aatagagaag aaaaatcatt
     6301 tttgtttatt ggaataaaag cagaaatttt ctattttggc agggtgtata taatataata
     6361 ctaataactg cagtgttttg gacaaggtaa agttcaggga gaatggttac aggagtatcc
     6421 cttgcaatga ataaatagga aaattgtcga tgaggtataa ttatggtata ataagtccac
     6481 gaaaggagtt gtaaattatt cctcagatta ttccaattcg agatttaaaa aacacaagta
     6541 aaatatccca aatgtgtcaa gaagcaaaag aacctattta aacgaggatg atatttatta
     6601 acttagtgaa ctacaccgcc gtttatggca aagaatttgg aaatacggat atttgattgt
     6661 agctaaccac tgttatatta gttacaatat ataatatcat ttaatatata tcacaaaagt
     6721 taaatttata atcctaatat aggaggtatt accaatgaaa ttaacattta atggaacttc
     6781 tgccgctgaa ggttttcctg cactattttg cgaatgcaga cactgccaaa aagccaggga
     6841 gcttggtgga aaaaatgtac gtacgaggac ttcctgtctg attgacgacc aatatcttat
     6901 tgacttcccg ccggatacat atatgcatgt tctgcatgga aagctgaagc tttcaaaaat
     6961 taagcacatt attgttacac attcccatga agaccatttc caaccttatg agctgcttat
     7021 gagacgtccg ccttttgctt acccggggga agatgaaatt ctttatgtat acgggaacga
     7081 tggagtatac gaaaaatttg ttcaagccgg ttgcaccaat gaaaacaaca aaaaatattt
     7141 acaatacgtc catgtttatc cgggaaaaca gtttaaggct ggtgaagcaa atgtgcttcc
     7201 tctcagagcg gatcacacta atggcaacga aatttgccat atatatatta ttaatatagg
     7261 agaaaaacat cttctttatg gccatgatac cgggtatttt cctgaagaaa catgggaagc
     7321 acttagaagt atcaaacttg atggtgtgat acttgattgt acttgcggaa caattgaatg
     7381 tgaaagaggt catatgggta ttactgcaaa tgttaaagtt aaagaacgcc ttatagaaca
     7441 aggcagcgca gatgaaaaaa caacatttat tgttactcat ttttcccata actgtggacc
     7501 tttatatgac gatatggtta agcttgcagg agaaaatgga tttattgcaa cttttgacgg
     7561 gatggaaatt aatatataaa ataatacata aataatatat atcaatatat atatcaataa
     7621 tatgtgatca ataatatgtg atcaataata tgcgatcaat aatatgcgat caacaatatg
     7681 tgataaataa tatgcggtaa ataatatgtg aaattaataa aaaagaattt acagaacaac
     7741 ttgacctgtc tataaaaaat gggggggaag ccccccctgc aagtgactta agcattattt
     7801 gtcttaccct gaggcattaa ctatctgacc ctgaagtatt ggctatctca ttctaaagca
     7861 tcaactaact taccctgaag ctaagatttg taaccggtcc ctttacattt ccactattta
     7921 cagcccaaat agagatatca tagttgccgt acggagcatc atatggaata tagtaattca
     7981 aactgaacga tgtatcactt tcctttttta atacctcaaa tattccatac attgctactg
     8041 aaagataaac tctgcttatt tcttctgtag cttcttcaat gctgactttt aaagatactt
     8101 ttgtacctgg ggaaccttcg gctggagtat caattgcctt tagtttataa ctgaaatttg
     8161 acattatatt cacctccctt actcagtcgt tttatactat tttgccaacc tgatatgcaa
     8221 aaacttaaat tgtaagtatt tcctaaagta tcggaaagac ttagcggtca gggcgttata
     8281 accatatatt aattacttgg gaaataatcc cttcataatc atattgcatg catgttcagt
     8341 aatactatta cggtccacag agctggaatt catcaggtaa tatttgcaaa tcccattaat
     8401 cattccaata atacctgcag ccaacaaatt aaaatcactg taacaaatta ttccttgctt
     8461 atatccattc gacagaagct ctgctaaaaa ctcaatatac ttatctttag aatttttaat
     8521 ttccttaaga acgtcatcgt caattccact tgttatttca ttaagaacta tgtttgctaa
     8581 ttcataatag tccagatagt agtcaatatt tgttttaatc atggatttta taatattttc
     8641 aatggaatca tcagaactat tagctaaact aataaggtcc tttactaaat aatcaattcc
     8701 ctctacaaca acggttctga agagttcgga cttgttttta aagtaataat ataaagttcc
     8761 ttttgctaca ccggcttcta cagctatttc atccatagaa gctttataaa agccttttct
     8821 gctgaatata cttcctgctg catttataat ttcatttctc actttgatac ctatctccat
     8881 tctaaaaaaa tctttaatgt aaatattgaa tataaaacaa ataaagtttc aacataattt
     8941 gttatactga ctggtcagta caatattatc agaatgaata aaataagtca agggttttta
     9001 cagtttccta cggttttcta aaaattcata aatagcggat ctacaccaca cttttcataa
     9061 atgtaaccgg ctttaaatac aataatttcg ataattctct atatacttat ggaatattac
     9121 gataacatct aatataatat gtatattgat aaaaattggg tgcgagttat ccaagggagg
     9181 ttttaattat gttttgtcca aaatgtggcg cacaattatc cgatgccgcg aaattctgta
     9241 acaagtgtgg ttctaaatta gagaagaatg ggtctaatct taatattgag caaaattcag
     9301 gacaaactac agggcaatat tcaggacaaa ctacaggaca atatgcaggc caaacggctg
     9361 aacaaagcag ttatgcttca cctgttcaaa ctgaattgaa ttacaagatt tttggagata
     9421 acatgcctgc agtttcgata aggctaaaca ggggagaaag tatttttacc cagtccggtg
     9481 ggatggtgtg gatggatagc ggtataacta tgataacaaa tatgcagggc gggttcatga
     9541 aagggcttgg acgcatgttt agcggagaat ctctttttat ggctacatat acctcaaatg
     9601 ctccaaatca ggaaatagtt attgcaagca cattcccagg tcatatagtt gatattgatg
     9661 ttggcagaac tcctattata gcacagcggg gagcgttttt atgtgcacag ccaagtgttt
     9721 cgattaacgc atatgttgtt aaaaatatgg gtgctggttt cttcggtggg gaaggttttg
     9781 tacttcaaag aataactggt acaggcatgg tattcctaga agtagatggc agtttagtag
     9841 aacgaactct tgcaaatggc gagactatca aagtggatac aggtaatgtg gcagcattcg
     9901 aggagagtgt gcaatatcag gtggaaatgg ttaagggttt taaaaatgta ttgtttggcg
     9961 gggaggggtt gtttttaact acattgagag gacctgggaa ggtttggctg cagactatga
    10021 ctatgcccag ctttgcaaaa caaatcatac cgtttttacc gaaatcgtcc aattgattta
    10081 gttaaattat ttgcttgatc aatttaataa tcaaatcaac aaattaacta gctaaactgg
    10141 tgtgcgctgt actgcgtact gtaacacgta ctgtaacgcg tgctgtattg cgtactgtac
    10201 tgcgtaatgt acagcagaaa ttgccgcagt acagtacgaa gcaacagtga gtggcagccc
    10261 tttaatttgt attgctttca tgttaagatg ttcatacctg cctgctaaac atcctgctca
    10321 atttataaag cctttcccca gcatactcaa agaaattata ataggattca caatagtaat
    10381 tgagtaccgg caatccatcg ctaaaaggtt cccttgtcct tcggcatcct cccctgcaga
    10441 aattaaacca tttacactct ttgcatttat catcgacatg catagatacc tcaatgaatc
    10501 ttttccctgt atcagtattt aacagctcat gaaagtcatt ttcctttata tttcccagtt
    10561 tccactgatc tgttacataa aaatcacaag gataaacccc tccatcggct tctattacaa
    10621 aatagcactg gcacctgccc atcatgccgc atgtttcagg gggatgaccc atcatcattc
    10681 ctaccaggtt gtcaaaataa cggatactga ctatttcacc ttttgtaata tcattatacc
    10741 agagatcaaa aaggcctttg agaaaattgg agtacatcaa cggcgtaaga gaataagaat
    10801 gtccccccgg tttctcatca agagggtcaa ggcattgaat aaactgcaaa tatttgaaat
    10861 tatatttttt gaagaaattg tatactttgt ttgtatgcct tgcaactgaa ttatttacta
    10921 cacttaatat attatactga acgccatatt tattgaatat atttacagta ttcatgattt
    10981 ttgaaaaagt cccgtcaagt gtatggttaa ccctgtttac atcgtttatt tcctttggcc
    11041 catcaagtga taaccctacc aaaaagttat ttttaaaaag aaaatctgcc cattgctcat
    11101 ctattagagt tccatttgtc tgaatagcaa aatgcacacc aattttcttt gtattatatt
    11161 tatttacaag ctctacaaag cttttgtaga attcaagccc ggcaagggtg ggttcacctc
    11221 cctgaaaagc aaaggtgcac atattttctg cataatcaaa agactttctt ataagtaatt
    11281 cagctgtttc tatattcatc ataccatacg acgctattga acgattttcg gcaatagaat
    11341 ggtaaaagca gtatttacat cgcagattac aactacttga tgcgggtttt atcagtaaac
    11401 ttagtggcgg catttgtaaa atcctcctgg taaaatataa gtaaaacata gggactaagg
    11461 aacataggga ctgttattat ttcgtttcag tctgcagttg atgcatgagg gcaaacaagc
    11521 tgcctccatg gctgaaccgc ttatataact agactgtccc tattgctatt gtactatttt
    11581 tctattattc cagcaccaaa ttgccaaaaa attcggatct atgaaaatct ggagttatta
    11641 ttgttatagg attccagcac ccataatgtg ggaacccggt ttcatctcca cacttatata
    11701 gattggcttc aagtctgcat ccaggtttaa aattcaactt tccaaagtgt tcttcaagaa
    11761 atgtgaatgg tatggagtat tgtgctgtcc agaattcccc attgtagttg gcgagattct
    11821 cttttgtaac agatgtttta atgttgaaga attcccggaa attatctatt acaaatcttt
    11881 ttcttccgtg cctgttgatt cctaaaccta gaagaagggt acctgcagca ttcatctcaa
    11941 aattgaaata cctgtcatct ttatccggat tggggtttat gaaaaattca acgcagctat
    12001 ctttatatac atcttcatcc atattaaaat atttaacttt aatttccttt tcaaaagact
    12061 tgaataaaat gtggaaatga gtatcagtgt aaaataattt agccattgtt tttggcttat
    12121 agccattatc ttcccaaaga aagcagtcga tatttatttc ttctatgctg tcccatgctt
    12181 tttctattgg tgtaacatta agctcaattt ttttaacagt atatgttttt ttcataaatg
    12241 tctatcctcc tgcctagatt tttatttatt tcccactctt caataataca tttccgaggg
    12301 aaaagggtca agtgccagga acgaccttta gatacttatt ctaaatactt ccacaaaaaa
    12361 ggttccgccg aaaaatttca aacaaaaaat ttcccccttc ttctattaag tttaaattcc
    12421 tttaaatatc tcctgttttc ttagttttgc
//
LOCUS       DAOSDD010000128        12339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_1980_length_12339_cov_4.990720, whole genome shotgun sequence.
ACCESSION   DAOSDD010000128 DAOSDD010000000
VERSION     DAOSDD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_12339_cov_4.990720"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..273)
                     /locus_tag="PK733_11725"
     CDS             complement(1..273)
                     /locus_tag="PK733_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOJ11247.1"
                     /translation="MPRRDGTGLIGAESMTGRGLGLCTGANVVKHGAGLGMGLGLACR
                     RGFGRSFGRGFAINEVSSKIQKELLHEQKDILQNRLEVIDKQLENL"
     gene            complement(746..1108)
                     /locus_tag="PK733_11730"
     CDS             complement(746..1108)
                     /locus_tag="PK733_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ11248.1"
                     /translation="MSNKIPSIKKEILRVAAYCRVSTVYEEQQSSLNALIQYYTDYIN
                     NHDGWILAGIYFEQASGTRFDNRDEFNRILKIIMRPLLVKKFLRKFNAGRVHRIVRMS
                     NTSPENFLSLPTLRLNHG"
     gene            complement(1171..1431)
                     /locus_tag="PK733_11735"
     CDS             complement(1171..1431)
                     /locus_tag="PK733_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOJ11249.1"
                     /translation="MKKAWTDEAWEDYIYWQTQDKKTLKRINEILKDINRDPFSGIGK
                     PEPLKHKRQGYWSRRIDEGNRIVYKVECDQIVIVQCKAHYDD"
     gene            complement(1434..1688)
                     /locus_tag="PK733_11740"
     CDS             complement(1434..1688)
                     /locus_tag="PK733_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOJ11250.1"
                     /translation="MAKTINVTIRLDREVKENAEKLFNDFGMNLSTAFNIFARQALRQ
                     GKIPFEIYDPFYSEKNQARLESSIKHAEAGQVTVHELIED"
     gene            complement(1913..1988)
                     /locus_tag="PK733_11745"
     tRNA            complement(1913..1988)
                     /locus_tag="PK733_11745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1953..1955),aa:Ala,seq:ggc)
     gene            2346..4196
                     /locus_tag="PK733_11750"
     CDS             2346..4196
                     /locus_tag="PK733_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOJ11251.1"
                     /translation="MPLDGIVADCIVLELENTLTGGRIDKIFQPEPDEIVIYIRARGV
                     NNKLLLSANPNYPRIHLTEVSKNNPSNPPVFCMLLRKHLSGGKVLGFDFHNYERIISI
                     NIESINELGDLSAKKLIIEIMGRHSNIILVNENGKIIDSIKHVSSDMSRVREVMPARE
                     YVLPPGQDKINPLNLDMYALFGNESTGLYKSAGLSVEKYLLNTIKGFSPFLCREICLG
                     AGINGKQSLSEVVENSGAFSAIKDAISAAILRIRGRDYRPYIVFQDNKLDIPLDFHCL
                     PLNKSINKPATVEYFSSISQVLDVFYSKKDFGEKLNHKKSVLIRVLDTHLDRCNKKLA
                     LQQESLREVSDREKLKLYGELLTAFIYSIPHNSKKVSLPNYYNQDNEYIDIPLNEYKT
                     PQENAQIYFKRYAKAKSTFEHASIQVKETIEEIEYLECVYHLLVNCTAFKEIDEIRDE
                     LVEQEYLTSKGKTRKGNASQGNTAHKRLSGQSSKTSAMSSPLHYKSSDDFDIYVGKNN
                     KQNDFLTFKFASSRDIWLHTRNIPGSHTIIRCQNKDIPDATLEEAAMLAALHSKAGKS
                     SKVPVDYTNVKYVRKPSGAKPGMVIYENFKTYIVTPDEKLAEKMAVKPAQ"
     gene            4474..5655
                     /locus_tag="PK733_11755"
     CDS             4474..5655
                     /locus_tag="PK733_11755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ11252.1"
                     /translation="MISEKIAHNLGSSSWIRAMFEEGEKLRKIYGPDKVYDFSIGNPE
                     TEPPAGVKKALESIVSGNKTGVHRYMSNAGYPDVREKVAQYINKEKGSSLTADNIIMT
                     CGAAGALNVVLKTILNPGEEVIVFSPYFVEYLFYIDNHGGKPVVVPTVSDTFEPDIDA
                     FEKQINSRTKAVIINSPNNPTGIIYSEKMLMKMAEVINKKEKDFNSTIFLIADEPYDK
                     IVYDEATVPSVLKIFKNSIIVNSFSKSLALPGERIGYIAANSLIDNISLLMSGFVFAN
                     RTLGFVNAPAIFQKVIADSLDQAVEVDGYKKRRDVLYNGLIDLGFQCVKPEGAFYLFP
                     KALIDDDVEFAKKAVKHNLLVVPGTGFGYPGYFRLSYCISLKTIENSLPAFKALVEEF
                     K"
     gene            complement(5781..6428)
                     /locus_tag="PK733_11760"
     CDS             complement(5781..6428)
                     /locus_tag="PK733_11760"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOJ11253.1"
                     /translation="MKKELKALIVCSGKYEDYSFYSSYIKDSDFIICVDGGASHIRKM
                     GATPHLLLGDFDSIDLEDYNYFSDLGVEIKEFPREKDETDTELAVDYAVNAGCREVTI
                     IGGIGTRFDHTLANIFLLKKMLNKGVEGWIINEYNRITLIDNKMCLKREDGIKVSLLP
                     LSEMVEGITTSGLYYPLSNAKMEIGPTRGISNEFSDEYAEISITSGLLLVILAKD"
     gene            complement(6652..7311)
                     /gene="rpe"
                     /locus_tag="PK733_11765"
     CDS             complement(6652..7311)
                     /gene="rpe"
                     /locus_tag="PK733_11765"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ11254.1"
                     /translation="MIKIAPSLLASDFSKLGEEIKKVEKAGADLIHIDVMDGHFVPNI
                     TMGPPVVKCLRPVTGLELDVHLMITDPDKYIDDFAAAGADIISVHAECCYHLHRTIQS
                     IKKKGIKASVALNPATSLSAIEWILDDVDMVLLMTVNPGFGGQSYIDAVTEKVRQLRK
                     LTKQKGLNTDIEVDGGIDLSNIYRVTEAGANVIVAGSTVYKAENTEKIISDLKNMAYK
                     G"
     gene            complement(7333..8184)
                     /gene="rsgA"
                     /locus_tag="PK733_11770"
     CDS             complement(7333..8184)
                     /gene="rsgA"
                     /locus_tag="PK733_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ11255.1"
                     /translation="MDKSSGMNVDYKTYECKARGVFRKDGMTPLPGDRVLFTVTDENG
                     EKGFIDEILLRKSVLARPAVANVDQVIAVIAACSPDPDLLLLDKLLITASRKDLDVFI
                     CINKIDLDPVMKYGSIIAAYEKAGYFVVPVSSKTDTGYDCLISKLKGRVSVMAGQSGV
                     GKSTILNKITGSLSMETGDISGKIMRGRHTTRHAQLFELDSGGYIVDTPGFSLLDLPD
                     TKPEELQFYYPEFHEFSCMCRFRGCSHIAEPGCLVKESVNSGKFDFQRYQRYILLYNA
                     LKQNKTF"
     gene            complement(8394..10331)
                     /gene="pknB"
                     /locus_tag="PK733_11775"
     CDS             complement(8394..10331)
                     /gene="pknB"
                     /locus_tag="PK733_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOJ11256.1"
                     /translation="MESNVLGNRYELIEKIGGGGMALVYKARCRLLNRYVAVKILRDE
                     FTGDEEFVKRFEVEAQSAASLSHPNIVSIYDVGNQNNTFYIVMEYVEGLTLKEYIADK
                     GSIEWKEAVKIAIQICSAIEHAHSKQIIHRDIKPQNILMTKDGIAKVTDFGIARAVTS
                     STITIVGSTIGSVHYFSPEQARGGYIDEKSDLYSLGIVIYEMITGKMPFVGETPVAVA
                     LKHIQDKPLQPIELKKEIPKALNNIVMKAIKKDQSLRYQTASEMLEDLYRVLKEPNGN
                     FAEYEDVYTNPTRKMKAIDEFELIKEEEYDIKGNKRRKQAQKPKKGDKASVWLAVIIS
                     VIVISVFSYIGYSVIVTSMSPESRKFIIKSYVNSNFNDVENELKSANFDVIEHWIYDD
                     TVEKNIIIKQSVEAGTEFKMEGYKTIEFYISKGPELVKIPSDLAGKECRKAEIDLQQL
                     NLKPKVVEEYSDVFPVDHVIGTYPDTGEEVKPETEVIIYKSLGPDIKKVAVPELSDRT
                     YSEAQVILEEHNLNVGELYPEGRSPGSSKIIKQEPAAGELVDEETAVNLYFEQAKYVS
                     IPVKLNEPERYADYVKVVAQITPSNTGETRIIYERNVEKGYFPITIEDIPVPENGSTK
                     LRVLINDGIYMEITLEWSALE"
     gene            complement(10351..11079)
                     /locus_tag="PK733_11780"
     CDS             complement(10351..11079)
                     /locus_tag="PK733_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOJ11257.1"
                     /translation="MYGIKSHKGMVREINEDSCNVIFGSSKMKAAFIIADGMGGHNAG
                     EVASRMAVEYISEKINEAEKDFSEDGVCQFLISTIKEANDIIFKKSNAPGPFSGMGTT
                     LIIAVFFAERVYIGHVGDSRVYVIRNNGIQQLTTDHSYVEELIKNGSLSRVEAENHPQ
                     KHVITRAIGCFEDVEADIYTYDIHTDDTFVLCTDGLTNMLSDDIIKSIIIQSDDPQSA
                     CAELVEKANKSGGNDNITLCIIRV"
     gene            complement(11105..12166)
                     /gene="rlmN"
                     /locus_tag="PK733_11785"
     CDS             complement(11105..12166)
                     /gene="rlmN"
                     /locus_tag="PK733_11785"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOJ11258.1"
                     /translation="MDEKPDLLSLSIEELKVFLSNLGQESFRTKQITRWLSAGIKDFN
                     EMTDLSKDFRNMLKKSACIGRLEVYRKAQSEIDGTTKYIFRLSDGNIIESVLMVYKYG
                     LSACISSQVGCRMGCRFCASTGLGLVRNLSSGEMLDQILSIQKDCGSRIGNVVIMGIG
                     EPFDNYDNVMKFIRQANLPEGLNLGYRHITISTCGLVPRILALAKEQIPINLSISLHA
                     PNDIKRQQIMPIGKIYSIDKIVEACKIYTETTKRRVTFEYALVDGFNDSEGDALELAR
                     KIKGMLCHVNLIPLNKVEGTGLRPVNRQKAEMFMRYLENYNIAVTIRRELGTDINAAC
                     GQLRRNVIEDKNAMTNNGN"
BASE COUNT         3643 a         2520 c         1991 g         4185 t
ORIGIN      
        1 ttataggttc tctaattgct tatcaataac ttcgaggcgg ttttgtaata tatctttttg
       61 ctcgtgaagc aactcttttt gtatttttga agatacctca ttaattgcaa aacctctacc
      121 aaaactacgt ccaaaaccgc gtctacatgc aagtcctaat cccattccga gaccagctcc
      181 atgctttact acattagcac ctgtgcaaag tcctaaacct cttccagtca ttgattcagc
      241 accaattagt ccggttccat ctcttctagg cataccattc acctccttat agtttatgac
      301 atatgccatt ttcaattctt attatactct atttctgaca tatgtcaata acttttatgg
      361 gaattttttt cacatcaaag agaccgccga tcatatgtat aaaacaatag taaacccctt
      421 acttatatag ttccttgaat aaaaaccatg tttcttcatt tttactggca acgaacaatc
      481 atgaaatgga caatgctcag gtggttctgg aaatatatcg ctgtttctgc ttgaaaaatc
      541 ttcaggtaaa tgattactaa aaaaacaatt tgcattatga tgcacctcct aacggaaatt
      601 atgcccataa ggagaatgct accatagcgt gaaaaaatat ttccacaata cgatagtatt
      661 aaatgcttta tcacattatt taatttgaga aaaaaagcct cttttcttaa attttaaaat
      721 gagaatttac attatatctc aaaagtcaac cgtggttcaa tcgcaaagta ggtagagata
      781 ggaaattttc tggcgatgtg ttgctcatcc gaacaattct atgaactctt cctgcgttga
      841 acttcctcaa aaatttcttt actaataatg gcctcatgat tattttcaat atgcgattga
      901 attcatctct gttatcaaac cgggtaccag atgcttgttc aaaataaatg cctgcaagta
      961 tccaaccatc atgattgtta atataatctg tataatattg gattaatgca tttagactac
     1021 tttgctgttc ttcatatacc gtactaacgc gacaataagc agcaacacgt aaaatttctt
     1081 tttttatgga cggtatctta ttcgacaaga aaatgtagct tttaatatgg aaagtcaggt
     1141 caaaaaagat gccattgttg aaatatgcct ttagtcatca taatgagctt tgcattgaac
     1201 aattactatc tggtcacact cgaccttata tacgattctg ttcccttcat caatacgtct
     1261 gctccaatac ccttgccgtt tgtgtttcag tggctccggt tttcctatac cagagaatgg
     1321 atcccggtta atatccttca aaatttcgtt gatacgcttc aaagtttttt tatcctgcgt
     1381 ctgccagtaa atataatctt cccaagcttc atctgtccat gcctttttca taatcaatcc
     1441 tctatcagtt cgtgtactgt tacctgacct gcctcggcat gcttaatcga actttcaagc
     1501 cttgcttggt ttttttcact gtaaaaaggg tcgtaaattt caaatggaat tttaccctgg
     1561 cgcagagctt ggcgtgcaaa tatattgaat gctgtggaaa gattcatacc aaaatcgtta
     1621 aacaactttt cagcattttc ttttacttcc cggtcaagtc gtattgttac attaattgtt
     1681 ttagccacaa aaaccacctc catctttaac tgtaaattaa ttataccata atatatcgtg
     1741 cgtttcaagt aatatgcact atattcgcta cctttcttgc cttcagaaat tatatactaa
     1801 ccactatatt ttgtattaga taaggctgtt cattgaacag gttggcaata tttaccatat
     1861 aaaaatgaaa gaagccaaac gcatcagcaa tcgacttctt tcgacaaaat tttggtggag
     1921 cataggggac ttgaacccct gacccccaca ctgccagtgt ggtgcgctcc caactgcgct
     1981 aatgccccat atttcccatt aaatgctata tttattaaca tatagtcaac aatattaaaa
     2041 gcaggatttg ttaattcaat tcctgctata ttataatata aatacttgca tttgacaaga
     2101 cctatttttc ccgggctttt tcccattttt tccaggtttt ttaagttttc gcattttaat
     2161 ttcttggagt ataggaattt ataaatctca tataaaattc ataaacatca atatttattt
     2221 cttgtaataa ttttgccaat tatttttttg ccaaacacta atactaatgc tataatagct
     2281 tataatatcc tgtaatgttg gattatatat acatatacac tattattgtg ttgggagtgt
     2341 ttgaattgcc attagacgga attgttgccg attgtatagt gcttgaacta gagaatacac
     2401 ttaccggtgg aaggattgat aaaatattcc agcccgaacc ggatgaaata gttatatata
     2461 ttagagccag gggggtaaac aacaagcttt tactaagtgc taaccccaat tacccaagga
     2521 tacacttaac agaggtttct aaaaataatc catcgaatcc tcctgttttt tgcatgcttt
     2581 tgagaaaaca tttatcaggt ggtaaggtac ttggatttga ctttcacaac tatgaaagaa
     2641 ttatatcaat aaatattgag tcaataaatg agcttggcga tttgtctgca aagaagctga
     2701 ttatcgaaat tatgggacgt cacagcaata taattcttgt gaatgaaaac ggaaaaataa
     2761 ttgactcaat taagcatgta agtagtgata tgagcagagt aagagaggtt atgcctgcca
     2821 gggaatatgt attgcctccc ggtcaggata aaatcaatcc tttgaatttg gatatgtatg
     2881 ctctctttgg taatgaatct accgggttat ataaatctgc cgggttatct gttgaaaagt
     2941 atcttctaaa tactataaaa ggattcagcc cttttctgtg cagagaaata tgtcttgggg
     3001 caggtattaa tggcaaacag tccctttcag aagtagttga gaattctggt gcattttccg
     3061 ctataaaaga tgcaatcagt gcggctatat taagaataag aggtagggat tacaggcctt
     3121 atattgtttt tcaagataat aaactggata taccattgga ttttcactgt ctccctttaa
     3181 ataaatctat aaataaacct gcaacagttg aatattttag ctctattagc caggttttag
     3241 atgtttttta ctcaaaaaag gattttggtg aaaaactaaa tcataagaaa tcagttttga
     3301 tcagagtcct tgatactcac cttgatagat gcaataaaaa gcttgctctg cagcaggaat
     3361 ctctcaggga agtatctgac agggaaaagc ttaagcttta tggtgagctt cttactgctt
     3421 tcatatattc tattcctcac aattcaaaga aggtttcttt gcccaattat tacaatcagg
     3481 ataatgagta tatcgatata cctttgaatg aatacaaaac accgcaggaa aatgctcaaa
     3541 tatattttaa gcgttatgct aaagcgaaaa gcactttcga acatgcatca atacaagtga
     3601 aggagactat tgaagaaatt gaatatttgg aatgtgttta tcaccttctt gtaaactgta
     3661 cagcttttaa ggaaatagat gaaataagag atgaacttgt ggagcaggaa tatttaacct
     3721 caaagggcaa aacaagaaaa ggaaatgcct cgcaaggaaa cactgcacac aaaaggctgt
     3781 cgggacaatc atcaaaaact tcggctatgt cttcccccct gcactataag tcttctgatg
     3841 actttgatat ttatgttgga aaaaataaca agcaaaatga ttttctcact tttaaatttg
     3901 catcttccag ggatatctgg cttcataccc ggaatattcc gggctcccat acaattatta
     3961 gatgccagaa taaggatatt ccagatgcca ctcttgaaga agctgccatg cttgcagctc
     4021 tgcatagcaa agcaggtaag tcttccaagg ttccggtaga ctatactaac gtaaaatacg
     4081 tgagaaagcc atctggtgca aaacccggta tggtaattta cgaaaacttt aaaacatata
     4141 ttgttacgcc tgatgaaaag ctagctgaga agatggccgt aaaaccagct cagtagattg
     4201 aaaagtagat ggaggagttg attgaagtgt agtatcatat tcactatagc aaattaaatt
     4261 tgatataatt gatataaata gatagcagta ttaaataaag caaataaagc tattttatag
     4321 ataacacaca aaagaaactt cattataaaa gttttgtgtg cttatgttac cttagtgaat
     4381 acgcaaaaat ttaatttgtt ccttgtagaa tcatttttgc gttatatata gcaaaataaa
     4441 taatttattg agatggagtg attaacaatg aacatgattt ctgagaaaat tgcacacaat
     4501 ctgggtagtt cttcatggat tagagccatg tttgaagaag gtgaaaagct tcgtaaaatt
     4561 tatgggcctg ataaggttta tgattttagc ataggaaacc ctgagacaga accgcctgca
     4621 ggagttaaaa aagctcttga atctatagtt tcaggtaata aaacaggagt tcaccgttat
     4681 atgagtaatg caggctatcc tgatgtgaga gaaaaagttg cacagtatat taataaagaa
     4741 aagggttcct cccttactgc agataatatt attatgacct gtggtgctgc cggagctctg
     4801 aatgtggtac ttaaaactat tcttaaccca ggtgaagaag taatcgtatt ttctccatat
     4861 tttgttgaat atctttttta tattgataat catgggggca aaccggttgt tgttcctact
     4921 gtcagcgata cttttgagcc tgacattgat gcctttgaaa agcaaattaa ttccagaaca
     4981 aaagctgtta ttataaactc accaaacaat cccacaggaa tcatatatag tgagaaaatg
     5041 ctcatgaaga tggcagaggt tataaacaaa aaagaaaaag actttaattc aaccatcttt
     5101 ttaattgcag atgagcctta tgacaaaata gtttatgatg aagcaacagt accaagtgtg
     5161 cttaaaatat ttaagaactc gattattgtt aattctttca gcaagtctct tgcacttccg
     5221 ggtgagcgta tagggtatat tgctgcaaac agcttaattg ataatattag cttgctaatg
     5281 agcggttttg tatttgccaa tcgtactctc gggtttgtaa acgcacctgc tatttttcaa
     5341 aaggtaattg ctgattcttt agatcaggcg gtggaagttg acggttacaa aaaaagaaga
     5401 gatgttttgt ataatggtct gattgacctt ggtttccaat gtgttaaacc tgaaggtgct
     5461 ttctaccttt tccctaaagc tcttattgac gatgatgttg agtttgcaaa gaaggcagtg
     5521 aagcacaatt tgcttgttgt accagggaca ggtttcggtt accccggtta cttcagactt
     5581 tcttactgta tcagtttaaa aacaattgag aattctttac cagctttcaa agcattagtt
     5641 gaggagttca agtagacaat caagcaggga ctgtttttgg taacaaaaag agacggttcc
     5701 tgcttgtcat gggttttcag aaatatggca aaaaagtaga ctgcacgagc cctcttttcc
     5761 gagcagactc cctggttttt ttaatccttt gccaatatca ccaaaagcag gccgcttgta
     5821 attgatattt cagcatattc atcactaaac tcattgctga tgcccctagt aggtcctatt
     5881 tccattttcg cattactcaa tggataatag agaccggatg tagtaattcc ttctaccatt
     5941 tcacttaaag gaagcaggga tactttaatc ccatcctctc tttttagaca catcttatta
     6001 tctataagag taatccggtt gtattcattg attatccaac cttcaacacc tttattaagc
     6061 atcttcttta agagaaatat gtttgctagt gtatggtcaa atcgagtacc tatgccgcct
     6121 ataattgtca cttcccgaca tcccgcattt acggcataat ctaccgccaa ttcagtatcg
     6181 gtttcatctt tttcccgggg gaattcttta atttcaactc ctaggtctga aaaatagtta
     6241 tagtcctcta aatcaataga gtcaaagtcg cctagcaata agtggggtgt tgcccccatt
     6301 tttctaatat gtgaagcacc cccatcaaca caaataataa agtctgaatc ttttatatag
     6361 ctactataaa aagagtaatc ttcatatttt ccgctgcata ctattaatgc ttttagttcc
     6421 tttttcatac atatagctcc tatcagtgtc tgaatcagcg caagtcacaa caggaagaca
     6481 gttcttctgt gctagtattt cttgcttctg ttgtgtttta gaacaagaga accttaccct
     6541 tacctcttat atccttaccc ttatcctctt atccccttcc tcttatcctc ttatcccctt
     6601 actctttaca tttatctcct tattctgctt acgcccttta tgtcacctat tctacccttt
     6661 ataagccatg ttttttaagt cgctgattat tttttccgtg ttttctgctt tgtaaactgt
     6721 ggatcctgcc acgataacat tggcccctgc ttcggtaact ctgtaaatat tgcttaaatc
     6781 gataccgcca tctacttcta tgtcagtatt aagtcctttt tgtttagtaa gcttccttaa
     6841 ttgcctgact ttttccgtaa cagcgtcaat ataggattgc ccgccgaatc ctgggtttac
     6901 agtcattaaa aggaccatgt caacatcatc aagtatccat tcaattgctg aaagtgatgt
     6961 agcaggattt agagctactg acgcctttat gcctttcttt tttatgcttt gaatggtacg
     7021 atgcaaatgg tagcagcact ctgcatgcac agatattata tcagcacctg ctgctgcaaa
     7081 atcatcaatg tatttatccg gatccgttat cattaaatga acgtccagtt ccagtcctgt
     7141 aacaggtctc aggcatttaa ctacgggtgg ccccatagta atattaggaa caaaatgtcc
     7201 atccataaca tcaatatgta ttaaatctgc tcccgctttc tccacttttt ttatctcttc
     7261 acccaatttg gagaaatctg aagcaagtaa tgatggtgca attttgatca tatataaaca
     7321 ctctccctta tgttaaaaag ttttgttctg ctttagggca ttatatagta aaatataccg
     7381 ctgatacctc tgaaagtcaa actttccgga atttacactt tcttttacca ggcagcctgg
     7441 ttcagctata tggctgcagc cccgaaatct gcacatgcag gaaaattcat gaaattcagg
     7501 ataatagaat tgaagctctt caggcttagt gtcaggaagg tcaagcaaac tgaaacccgg
     7561 agtatctaca atgtatcctc cggaatccag ttcaaacaac tgtgcatgtc tcgttgtatg
     7621 acgtcccctc attattttcc cacttatgtc ccccgtttcc atagataagg atccggttat
     7681 tttatttagg attgtggatt ttccaacacc ggactgacct gccattactg atactcttcc
     7741 tttcagtttc gaaatcaggc agtcatagcc ggtatcagtt tttgaactga caggaacaac
     7801 aaaatagccg gccttttcat aagctgctat tatactgccg tatttcatta cagggtcaag
     7861 gtcgatttta tttatgcata taaaaacgtc caggtctttc ctactggcag ttattaaaag
     7921 cttatctaaa agcagcaggt cagggtcagg agagcaggct gcgattacag ctataacctg
     7981 atcaacattt gcaactgcag gccgcgccaa aacagatttt ctgagcaaaa tttcatctat
     8041 aaaacccttt tctccatttt catcagttac agtaaataac actctatcac cggggagagg
     8101 cgtcatacca tcttttctaa agacacccct ggctttgcat tcgtatgttt tataatctac
     8161 attcatacct gaactcttat ccaagttcct atccaagctc ttatctaagt ttttatcagc
     8221 ttccttatta tcagcttcct tattatcgga ttttacatag taaaaaccac caacaccttt
     8281 taatattgta cctgttatta taatccctcc atgtgccttg cgttacccaa actatagtgt
     8341 attatgccaa tatatattta tgtgtatttt actgagtaaa cctaaacttc actttattct
     8401 aaagctgacc attcaagggt tatttccata tatatcccat cattaatcag taccctcagc
     8461 ttcgtgcttc cattttctgg cacagggata tcttctatag taatagggaa ataacctttt
     8521 tcaacatttc tttcatatat aattcttgtc tcaccggtgt ttgatggtgt aatttgagct
     8581 acaactttca cataatcagc atagcgctcc ggttcattaa gttttacagg tatactcaca
     8641 tattttgctt gctcaaagta cagattaact gctgtttcct catcaaccaa ttctcccgct
     8701 gcaggttcct gtttgattat ttttgagctt cctggacttc ttccttcagg gtataattcc
     8761 cccacattca gattatgctc ttccagaata acctgggctt ctgaatatgt tctgtccgaa
     8821 agttcaggta ctgcaacttt ttttatatcc gggcctaagc ttttatatat aataacttca
     8881 gtttccggtt ttacttcttc acctgtatcc ggatatgtcc ctataacatg gtcaacagga
     8941 aaaacatcac tatattcttc tacaactttg ggtttaaggt ttaactgctg caagtcaatt
     9001 tccgccttcc tgcattcctt acctgcaaga tcagaaggga ttttcaccaa ttccgggcct
     9061 ttacttatgt agaactctat tgtcttatac ccttccattt taaactctgt tccggcctct
     9121 acactttgtt ttataattat atttttctca acagtatcgt catatatcca gtgttctatt
     9181 acatcaaagt ttgcactttt aagttcattt tcaacatcat taaagttgct gttaacatag
     9241 ctttttataa taaattttct tgactccggt gacattgacg taactataac actatatcca
     9301 atatatgaaa atacgcttat tactataaca gatattatta cagcaagcca gacacttgcc
     9361 ttgtctccct tttttggctt ttgagcttgc tttcttcttt tgttcccttt aatatcatac
     9421 tcctcctctt ttataagttc aaattcatct atggctttca ttttccttgt aggatttgta
     9481 tatacatctt catactctgc gaagttccca tttggctctt ttaaaaccct gtaaaggtct
     9541 tcaagcattt cggaagctgt ctgataccgt aaactctgat ccttttttat tgccttcata
     9601 actatattat taagggcttt tggtatttcc ttcttcaatt caataggttg aaggggttta
     9661 tcctgtatat gcttcaatgc aacagctaca ggggtttcac caacaaaagg catctttcct
     9721 gttatcattt cataaataac tattcccaga gaatacaagt ctgatttttc atcaatatat
     9781 cctcctcttg cctgttccgg agaaaaatag tgaactgaac ctatggtact tccaactata
     9841 gttatagttg acgatgttac agctctggca ataccgaaat ccgttacctt tgctattcca
     9901 tctttagtca taagtatatt ctgtggttta atatccctgt gaataatttg cttgctatgt
     9961 gcatgctcaa tggcggaaca aatctgtata gcaattttta ctgcttcttt ccactcaatt
    10021 gaacctttgt cggctatata ctctttcaaa gttaaacctt caacatattc cataactata
    10081 taaaaagtat tgttctgatt acctacatca tatatggaaa ctatattggg atgagacaag
    10141 ctggctgctg actgtgcttc tacctcaaat ctttttacga attcctcatc tccggtaaat
    10201 tcatctctta gaatttttac agccacatac cggttaagga gtctgcatct agctttataa
    10261 acaagagcca tcccccctcc accgattttt tctattagtt catacctgtt tccaagtaca
    10321 tttgattcca tggttacacc tcttataatt ctagactcgg attatacata gtgtgatatt
    10381 atcattcccg ccgctcttat tagccttctc aaccagttca gcacatgcag actgcggatc
    10441 atcactttgt atgataatgc ttttaattat atcatcagaa agcatatttg taaggccatc
    10501 agtacaaagt acaaaagtat catctgtatg tatatcgtat gtatatatat ctgcttctac
    10561 atcctcaaag catccaatcg cccgggtaat tacatgcttc tgcgggtgat tttcggcttc
    10621 aactcttgat aaagagccat tcttaataag ctcttcaaca tacgaatgat cagttgtaag
    10681 ctgttgtatt ccattatttc ttattacata taccctgcta tctccaacat ggcctatata
    10741 caccctttca gcgaaaaaaa ccgctattat caatgttgtc cccatacctg aaaatggtcc
    10801 tggagcattg gattttttaa atataatatc atttgcttct tttattgtgc ttattaaaaa
    10861 ttggcacact ccatcttctg aaaaatcctt ttcagcttca ttaattttct cactaatata
    10921 ttctacagcc attctgctgg caacttcccc ggcattgtga ccacccatgc cgtcagcgat
    10981 tatgaatgct gccttcattt ttgaagaccc gaaaattaca ttacaagagt cttcattaat
    11041 ttctctaacc atacccttat ggctttttat accatacatc acaaaaccac cgcccaagtg
    11101 aaagctaatt accattattg gtcattgcat ttttatcttc aataacgttt ctacgcaatt
    11161 ggccacatgc ggcatttatg tcagtaccca gttcccgcct gatagtaact gctatattat
    11221 agttttcaag atatctcata aacatttctg ctttttgtct gttaaccggt cttaaacctg
    11281 taccctcgac tttatttaaa gggatcaagt taacatggca taacatgcct tttatctttc
    11341 gtgccagttc caatgcatcc ccttcagaat cattgaaacc atcaacgagg gcgtattcaa
    11401 aagtaactct cctcttagtt gtctctgtat atatcttaca tgcttcaact attttgtcaa
    11461 tagaatagat tttaccaatt ggcattattt gctgtctttt tatatcgttt ggagcatgca
    11521 gtgatataga aagatttatg ggaatttgct ctttagcaag ggctagtatt cgcggtacaa
    11581 ggccgcatgt tgaaatggta atatgtctat agccaaggtt aagaccttca ggcaaattag
    11641 cctgccttat aaatttcatc acattgtcgt aattgtcaaa aggctcacct atccccatta
    11701 ttacaacatt tcctatgcga gacccgcaat ctttttgaat tgacagtatc tgatcaagca
    11761 tttcacctga agagagatta cggactagtc ccaaccccgt agatgcacaa aatctgcagc
    11821 ccatcctgca gccaacctgg gatgatatgc atgcagacaa accatattta tataccatta
    11881 gaacgctctc aataatattc ccatcagaaa gcctgaatat atactttgta gttccatcta
    11941 tttctgattg agctttccga tatacttcca gccttccgat acatgctgat tttttcagca
    12001 tatttctaaa gtcttttgaa agatccgtca tttcattaaa atcttttatt cccgctgaaa
    12061 gccagcgtgt aatctgtttt gttctaaagc tttcctgccc cagatttgaa aggaatacct
    12121 taagttcttc aattgaaagg ctgagaagat ccggtttttc atccatattt ccttattcat
    12181 tctccttatc tttattcctg cgcagatttc tgcactaaat catccttttc tcattaattt
    12241 tgctataaaa aacccgtcta ttccctctac attgggaaat agctggacat atccttcgcc
    12301 ggctgtttcc tttattaacc cctttggaag cagagtttc
//
LOCUS       DAOSDD010000129        12208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2008_length_12208_cov_5.052004, whole genome shotgun sequence.
ACCESSION   DAOSDD010000129 DAOSDD010000000
VERSION     DAOSDD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_12208_cov_5.052004"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK733_11790"
     CDS             complement(<1..248)
                     /locus_tag="PK733_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ11259.1"
                     /translation="MKARLLYWWRRDGIGYLFMLPFLVLLLIFIVIPVFVALGTSFTN
                     YNMLQKPRWIGLNNYKLLFMDDAIFLTALKNTFVFACIT"
     gene            complement(277..3204)
                     /locus_tag="PK733_11795"
     CDS             complement(277..3204)
                     /locus_tag="PK733_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11260.1"
                     /translation="MNSFSKKTIVVTLCFIVLIGVFSPVRGVSDVPLRDKFNYGTEKD
                     TMLEYYRTVRYPYYLSILEDYEKNGYMPAIASAVVVEPEKIRLSNGENAVLETGIGGS
                     SIPAFKWSEDIPWVEWDFMISTQGLYEIEIEYYMMPGSGNPAVRSLKIDGKVPFLEAV
                     NIVFNRGWKDEGEPVINSIGDEVRPGQVEVPKWRNVNMCDGQGLYSQPFQFYLESGEH
                     VLQLEYIDQPMAISQLRVKPVTKIPVYEQVKEQYIQKGYSNVSRTVEFQAESSAIEKS
                     DPAIRRESDGDPICKPASYLDRKLNVLGGYRWRRGNQAITWKFSVPEDGLYKIGIRAA
                     QWWNDGLPSFRQIAVDNRVPFKELEEYKFVFDRKWRTEVLQDDKGDPFLFYLTQGEHT
                     LTMTVKLGSLTPVIYSLNEDSILLSNILRDIIKVTGSNPDPNYDYDFFKTIPNLKNDI
                     ETLVQSLQKKHDMMKDISEKLPAMASNMLTIKSQLEGMVKDPFSIAKRINDLNSAQTN
                     FGNWYLSLQNQPLLIDYFLAGSPDEIWKNKQSNIFQKLYFTFANFLISFKKDYDNIGS
                     TISNDMEINKTINVWVARGTEWAELIKEMADEDFTPDTGIKVNINVFPPSQLQAGSVN
                     ALLLSINSGKAPDAALGVGSNSPVEFAIRDAVLDLGQFQDFSEVSDWFIPNIFIPYKY
                     REGIYAIPETMDFTVMFYRKDIAGELGLKLPDTREDLYSHVLPVLYQNGLQFYYPADF
                     THFIFQNGGEYYTPDGLKSALDRPEAFRAFREYTELYTHYAVPVSASFFNRMRTGEMP
                     VGIGSYGLYMQLSVAAPELAGRWGIAPLPGTLRPDGIVDRSNGALAGQCGIIIKQTKN
                     PEETWKFLKWWMSTPVQTRFARELEALIGVEARWNTANVNAFTSLAWKKEDLDVIQEQ
                     WKWAKEIPVILGGYFTGRHIGNAWNSVVIGGVPVRDAIEKAVEEINRELRMKQEEYGI
                     ITN"
     gene            complement(3524..6403)
                     /locus_tag="PK733_11800"
     CDS             complement(3524..6403)
                     /locus_tag="PK733_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11261.1"
                     /translation="MTKLNLWQSSKYKINDKISDKISNNSFKWNIIGISTIIIAAIIP
                     VVAMITYISSVNLVKSQVSQINTEMLEQLSRFIDGELMEQERLAITIIENDSVQDLML
                     NQDKHSLFEKQLLIPQAERTLLEVAIQGNVRNIYILNTSKELVITNDAYAKQLDFKNE
                     YYHVFSYADINEDKFVWIEPSEGISSYKSSSEGQGSLSLIKTIKSKIDNQKLGYIIIT
                     FHDKFLKKLYSSNDREMLDVFILSNKGNLVSSSSLSVNRYDDFLKRAINTDSSNSNIN
                     VDNNVIRLDTDSIQVFFIIMQRTGWKLVLCADYSKDLSNMRRIRDLILQTSGLLVVFF
                     IIVNFNILSVIIKPLYRLAKRFIPGGRKQLETKKYEQRNGILLTPYGISLKQMLVINF
                     TVVIILPVAILVLFEFSLFYKIIENKIIDSYSYYLEQTAIQTDSKFNRIENSLKNFYL
                     NNDILSLMMNSKCQGNEKYSELTKKPAQIIKEEQAINPDIMYINIFDRDFKLIYSTSS
                     SQQYFPKGLVDSALSKQDGETVYYGPYNDIFSRQVISIGKRIRKVLSLETAGFIHAVI
                     NVVPLKESITGRMNGAHTCIYSSDGRTIISSDNAVSFDAISNEEIFKANRGIFKSKQL
                     GTTIIFNKLKANKWIITLGVSDKYIRQKIWKALLSNYYILIIYTTFILLIIFLLVKRF
                     YTPINILNQCVEDIKARLSAGVKDEISIGNFSGKKNEIGQIGQILQYMENEIRKQANE
                     LYETEKLKKATELYALQVEINPHFLFNTLETIRWKAVDQGKGDNEISGMIYQLSEFLK
                     ITLKNKASFITVEEEIKYAKTFSNILKYKYEDDLDVFFDIDSRANQYRTIKMILQPLI
                     ENSIVHGFKEKTGKCIIIVSVKITENKIIYQIKDNGAGISKTRLEELKNSRFSNDKSG
                     RTSIGLKNINERICMNYGEEYGIDIESKPGTGTTVIVVIPADKFM"
     gene            complement(6476..7744)
                     /locus_tag="PK733_11805"
     CDS             complement(6476..7744)
                     /locus_tag="PK733_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11262.1"
                     /translation="MYKCIIAEDEKQIRNGLCNYFDWSEAGFNILGTAGNGKEALELI
                     KKYSPHLVITDIKMPVMDGLELMEHALKIDQSIKFIVLSGYDDFEYAKKAIAYGVCDY
                     ILKPLNTQELNQSLTKVYTKLSEETSKSSLFDKGISLLKDDFLKNIAKGIITGNNFIQ
                     KKAWELGININEYMFYVIRISIEDESFYTAGNKNSVRAISIKRNTVSNIARETINKHG
                     GSCIFNDYENQISIIFYISKRNMKNAYDILQKILRDVKKSSDDYINCKVHIGLGGEFS
                     DIYSIKGLYEKSDLNLQNAMMEKDFSNKNEGKIVNAAMLAIKKSELKDISLESIAKQI
                     FISPYYLSRVFKQETGENFVDYVTKIRIEKAKELISNFNLDNDQVADIIGYSSTKYFL
                     KVFKKCEGVTPKEFKKTNNKIVYQHKECIL"
     gene            complement(8254..9027)
                     /locus_tag="PK733_11810"
     CDS             complement(8254..9027)
                     /locus_tag="PK733_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ11263.1"
                     /translation="MNSSKAECQKNISVSLNELGVKEGAIILVHSSLKSLGYVPGGPE
                     TVISGILDALGDKGTLLIPALSYKYVNADPDNPVFDVLNTPSNVGAFPEYFRERQGTI
                     RSIHPTHSVCGTGFCADEILKDHILDTTPCGPNSPFRKLPEYGGKILFLGCGLRPNTS
                     MHGVEELAEAPYLFKDTRVYRIIHPDEHVTFMTSKLHYFNDHKLEQRYDRVESMLDGD
                     EIKSGKILDANAYLIDASALWKKALEIMRKDPYYFVEEI"
     gene            complement(9130..11790)
                     /locus_tag="PK733_11815"
     CDS             complement(9130..11790)
                     /locus_tag="PK733_11815"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ11264.1"
                     /translation="MIKAKNLEKTYEPKQVEDRLYNEWIEKDYFHAVIDHNKKPFTIV
                     IPPPNITGQLHMGHALDNTLQDILIRWKRMQGYCALWLPGTDHASIATEAKIVEAMAK
                     EGISKEMLGREKFLERAWEWRKHYGGRIVEQLKKLGSSCDWKRERFTMDKGLSEAVKE
                     VFVRLYEKDLIYRGERIINWCPKCNTSISDAEVEYEEKEGHFWHIKYPIKDSNEYIVI
                     ATTRPETMLGDTAVAVHPDDDRYKHLVGRTVVLPLVNREIPIIADDFVDREFGTGAVK
                     ITPAHDPNDFEAGIRHNLPQIKVINDDATMSENAGQYSGMDRYEARKQVVEDLKQLDL
                     LLKIEDHTHNVGECYRCKTVIEPLISKQWFVKMKPLAEPAIEVVKNGTIKFVPERFSK
                     IYYNWMENIQDWCISRQLWWGHRIPAYYCRDCGEMMVEREAPEKCTKCGSSNIEQDPD
                     TLDTWFSSALWPFSTLGWPEQTEDLRYFYPTDVLVTGYDIIFFWVARMIFSGVEHMGR
                     EPFKYVFIHGIVRDALGRKMSKSLGNGIDPLEIIDQYGTDALRFALVIGISPGNDLRF
                     SEEKIEAGRNFANKIWNAARFVLMNFDEELDFSKVDPGKFDMADKWILSRINALTREM
                     TDNLEKFELGIALQKIYDFIWEEFCDWYIEMVKPRLYDRESESRLEAQYVLNYVLKSA
                     MKLLHPFMPFISDEIYRHLIIEEPSIMISPWPEYSDDYNFPQDEERMGFIMEAIRSIR
                     NIRSELNVPPSKRAKVIFVTQEKDKKEIIEEGSNFIEKLAWASGTTIQSDKTSISVNA
                     ASAVIPGAEIYIPLEELIDIDKEIERLEKEKANLEKELERVNGKLNNPGFVSKAPKKV
                     VEEEKAKKTKYQEMYDKIVERIKGLENRVQ"
BASE COUNT         3565 a         2504 c         1770 g         4369 t
ORIGIN      
        1 gtgatgcagg caaatacaaa tgtattttta agtgccgtaa gaaatatagc atcatccatg
       61 aatagtaatt tgtaattgtt taaacctatc catctgggtt tctggagcat attgtaattg
      121 gtaaaactgg tgcccagtgc tacaaacacc gggattacta taaaaataag gagtaaaacc
      181 aggaagggca gcataaacag ataacctatt ccgtcccttc tccaccaata taataacctt
      241 gctttcatct taattcctcc tatcctatga tcatctctag ttagtaataa ttccatattc
      301 ttcctgtttc atccgcaatt cccggtttat ttcttctact gccttttcaa tagcatcccg
      361 cactggtaca ccgcctatta caacactatt ccatgcattt ccaatatgtc ttcctgtaaa
      421 atacccgcct aggattaccg gtatttcctt tgcccatttc cattgttcct gtataacgtc
      481 caggtcttct tttttccatg ccagacttgt aaaagcattc acattggcag tattccaacg
      541 tgcttctact ccaataagag cctcaagttc tcttgcaaat ctggtctgaa caggagtgct
      601 catccaccat tttaagaatt tccaggtttc ttcaggattc tttgtctgtt ttataataat
      661 tccgcattgt cctgctaatg caccgtttga ccgatcaact ataccatccg gacgaagagt
      721 acccggtaaa ggtgcaatac cccatctacc cgccagttca ggagccgcta cagacagctg
      781 catataaaga ccatagcttc ctattccaac aggcatctct cctgttctca tgcggttaaa
      841 gaaactagca gatacaggca ctgcataatg ggtgtacaac tctgtatatt ctctgaaagc
      901 ccggaaagct tccggcctat ccagagcaga tttaagcccg tcaggagtat aatactctcc
      961 tccattctga aatataaaat gagtgaaatc cgcaggatag taaaattgta gtccgttttg
     1021 gtaaagaacc ggaagcacat ggctgtaaag atcctccctt gtatcaggaa gttttaatcc
     1081 cagttcacct gcaatatcct tccgataaaa cattacagta aaatccattg tttcaggtat
     1141 agcgtaaatg ccttctctgt acttatatgg aataaatatg tttggtataa accagtctga
     1201 tacttcacta aaatcttgaa actgtccaag atccaataca gcatctctta tggcaaattc
     1261 aacgggagaa ttggaaccga ctcccagagc tgcatcagga gccttacccg agtttatgga
     1321 gagcaataat gcattaacgc ttcctgcttg aagctgactt ggaggaaaca catttatatt
     1381 tacctttata cctgtatcag gtgtaaaatc ttcatctgcc atttccttta ttaattccgc
     1441 ccattcagtt cctctggcaa cccatacatt aattgtcttg tttatctcca tatcattact
     1501 tattgtactg ccaatgttat cgtaatcttt tttaaatgat attaagaagt tggcaaaagt
     1561 aaagtaaagt ttctggaata tgttagattg cttgtttttc catatttcat ccggtgaacc
     1621 tgcaagaaaa tagtcaatga gcaaaggctg gttctgcaga cttaaatacc agttcccgaa
     1681 attggtttga gcactattaa gatcatttat cctttttgca atactgaaag ggtctttgac
     1741 cataccttcc aactggcttt ttatagtcag catattgctt gccatggcag gtaatttctc
     1801 cgatatatct ttcatcatat catgtttttt ctgcaggctt tggactaatg tttctatgtc
     1861 attttttaaa tttggaatgg ttttaaaaaa atcataatca taattggggt caggattact
     1921 tcctgtaacc ttgataatat cacgcaatat atttgaaaga agaatagaat cctcattaag
     1981 tgaatatata acaggagtta aggaacctaa tttaactgtc attgtaagtg tatgctctcc
     2041 ctgggtaagg taaaaaagaa aaggatctcc tttatcatcc tgtaaaactt cagttctcca
     2101 ctttctatca aacacaaatt tatactcttc taattcttta aagggcaccc tattatctac
     2161 agcaatctgc ctaaacgatg ggaggccatc attccaccat tgtgcagccc ttatacctat
     2221 tttataaaga ccatcctcag ggactgagaa tttccatgtt attgcttgat tgccccgacg
     2281 ccagcgatat cctccaagta cattaagttt tctgtctaaa tatgacgccg gtttgcagat
     2341 aggatcgcca tcactttctc tcctgattgc aggatcactt ttttcaatag cagaactctc
     2401 tgcttgaaat tctaccgtac gcgatacatt ggaatatcct ttttgaatat attgttcctt
     2461 aacctgttca taaacaggaa ttttcgttac cggttttact cgcaactgac taattgccat
     2521 tggctggtct atatactcta gttgcagcac atgttcacca gattccaaat agaactgaaa
     2581 aggctgtgaa tataaaccct gcccatcaca catatttaca tttctccatt tcggaacctc
     2641 tacctgacct ggtcttactt catctccaat actgttaatt acaggctctc cttcatcctt
     2701 ccagccacgg ttaaatacaa tatttacggc ttctaggaaa ggtactttcc cgtctatttt
     2761 aagagaacga actgcagggt tgcctgaacc gggcatcatg tagtattcta tttctatttc
     2821 atacagaccc tgggttgaaa tcataaaatc ccattcgacc cagggtatat cttcagacca
     2881 tttaaatgca ggtatgctgc ttcctcctat tccggtttcc aagactgcat tttcaccatt
     2941 agacaaacgt attttctctg gctcaactac tactgcagac gcaattgcag gcatataacc
     3001 attcttttca tagtcttcaa gtattgataa ataataaggg tatcttactg tcctataata
     3061 ttctaacatt gtatctttct ctgtaccata attgaattta tctcgcagtg gtacatcgga
     3121 aacaccccgg accggagaaa acactccaat taaaacgatg aaacatagag taacaacaat
     3181 tgttttctta gaaaaactat tcatgacctt caccctttat tttttattag agatgcacag
     3241 tttgcaccta aaatcttata attattcttg tacccggtgc tgtaacattt taccgaaaca
     3301 tattgacgct gaatgagttt tttgcaccag aatcaatatt tgcaactatt aacatacaat
     3361 agcatacaat gctatatagt aaacttaaac taactttttt aactaaagag tcagtttttt
     3421 tgtcattttg cttatatttt ataaagtttg atcaatatct gtattttaaa accctttaag
     3481 gcttgattcg aataataaat cgtgaattgt aaatagaatt ggcttacatg aatttgtcag
     3541 caggaataac tacgattacc gtagtacctg tacctggctt actttcaata tcaataccat
     3601 actcttcacc gtaattcata catattcttt cattaatgtt ctttagtcct atacttgttc
     3661 ttccactttt atcattggaa aaccggctat tttttaattc ttcaagtctt gttttactta
     3721 ttccggcacc attgtctttt atctggtata ttattttatt ttcagttatc ttaacagaga
     3781 caataataat acacttccct gttttttcct taaaaccatg tactattgag ttttcaataa
     3841 gtggctgaag tatcatcttg atagttctat attgattagc tctgctatct atatcaaaaa
     3901 acacatctag atcatcctca tatttgtatt ttagaatatt ggaaaacgtc tttgcatatt
     3961 ttatttcttc ctccactgtt ataaaactgg ctttattctt tagggttatt tttagaaact
     4021 ctgaaagctg atatatcatt ccactgattt cattatctcc ctttccctgg tcaacagcct
     4081 tccatcttat agtctcaagg gtattaaata aaaaatgggg atttatctca acctgaagtg
     4141 catataattc tgtagctttt ttaagctttt ccgtttcata cagctcatta gcctgttttc
     4201 ttatttcatt ttccatgtat tgaagtatct gtccaatctg accaatttca tttttcttac
     4261 cggaaaaatt tcctatgctt atctcatctt taactcctgc actaagtctg gcttttatat
     4321 cttctacaca ctgatttaaa atatttatag gagtataaaa tcttttgaca agaaggaata
     4381 ttataagtag aatgaaagtt gtgtatataa ttaatatata ataattactt agaagggctt
     4441 tccatatctt ctgcctgata tatttatcag aaacacccaa tgtaattatc cacttattag
     4501 ctttcaattt gttaaaaata atagtagtac ctaactgttt gcttttaaaa atgcctctgt
     4561 ttgctttaaa aatttcttca ttggatatag catcaaaact aactgcatta tcagatgata
     4621 taatagtcct gccatctgat gagtatatgc aagtatgggc tccattcatc cttccggtaa
     4681 tagattcctt taatggaaca acattaatga cagcatgtat aaagcctgca gtttcaaggc
     4741 taagtacttt ccgtattctt tttccaatag atattacctg acgactaaaa atatcattat
     4801 aaggtccata gtaaaccgtt tctccatcct gcttgcttaa tgcgctatca acaagtccct
     4861 tagggaagta ttgctgtgaa gacgatgtag aataaataag cttaaaatct ctgtcaaaga
     4921 tatttatata cattatgtcg gggtttattg cctgctcttc tttgataatc tgggcaggct
     4981 ttttagtaag ctcgctatat ttttcatttc cctgacattt actgttcatc atcaatgata
     5041 gtatatcatt attcaaatag aaatttttta atgagttttc tattctattg aatttagagt
     5101 cggtctgtat tgctgtttgc tcaagatagt agctgtatga atcgataatt ttgttttcaa
     5161 taattttata aaacaaagaa aactcaaaca atacaagtat tgcaacaggt aaaataatga
     5221 ccacagtaaa attaattaca agcatctgtt ttagactaat gccgtatggt gttaaaagta
     5281 ttccattcct ttgctcatac ttttttgttt ccagttgttt tctgccacca ggtataaacc
     5341 ttttggcaag tctatataaa ggctttatta ttactgatag aatattaaag tttactatta
     5401 taaaaaaaac aaccagcaaa cctgaagttt gtaatataag atcccttatt cttctcatat
     5461 ttgataaatc cttagaataa tctgcacata aaaccagctt ccacccggtt cgctgcatta
     5521 taataaaaaa tacctgtatg ctgtctgtat caagcctaat tacattatta tcaacattga
     5581 tatttgaatt actagaatct gtatttattg ctctttttaa aaaatcatca tatctattaa
     5641 cacttaaact acttgatgaa actaaatttc ctttatttga aagaataaac acatccaaca
     5701 tttctcgatc gttactgcta tataattttt ttagaaattt gtcatgaaat gtaataatta
     5761 tatagcccaa tttttgatta tcaattttac tttttatagt ctttatcaat gatagactac
     5821 cctgaccttc ggagcttgat ttatatgagg atattccttc agatggttct atccatacaa
     5881 atttatcttc attaatgtcc gcatatgaaa atacatggta atactcattt ttgaaatcca
     5941 gctgtttggc atatgcatca tttgttataa caagttcctt actggtattt aggatatata
     6001 tattccttac atttccttga atagccactt ccaataatgt cctttctgct tgcggaataa
     6061 gtaattgttt ttcgaaaaga ctatgtttgt cttgattaag cataagatcc tgtactgaat
     6121 cattttcaat aattgtaatg gccagtcttt cctgttccat aagctcgcca tcaataaatc
     6181 tgcttaactg ttcaagcatt tcggtattta tttggctaac ctgtgactta acaaggttca
     6241 ctgatgaaat atatgtaatc attgcaacaa caggtattat tgcagcaatt atgattgttg
     6301 aaataccaat aatattccat ttaaaactat tattactaat tttatcacta atcttatcgt
     6361 taatcttata tttagagctt tgccataaat ttaatttagt catagaaacc tctgacagat
     6421 atatactatg tttacaataa ttttttcaaa aaatgacatg aacatgcacc catacctaca
     6481 atatacactc cttatgttgg tatactatct tattatttgt ctttttgaat tccttcggag
     6541 ttacaccctc acactttttg aaaaccttca aaaaatactt tgtactgcta taccctatga
     6601 tatctgccac ttgatcatta tccaagttaa aattggatat taattccttg gccttttcga
     6661 ttcttatttt tgttacataa tcaacaaagt tttctcctgt ttcctgttta aaaaccctgc
     6721 ttaagtaata tggactaata aaaatctgtt ttgctatgga ttccagactt atgtccttca
     6781 actcactttt tttaattgcc aacatagctg cattaactat ctttccttca tttttattgc
     6841 taaaatcctt ttccatcata gcattctgca aattcaaatc tgatttttca taaagcccct
     6901 taatactgta gatatctgaa aattctccac ccagtccgat atgtacttta cagttaatat
     6961 aatcatctga agatttttta acatctctta ggattttttg taaaatatca tatgcatttt
     7021 tcatatttct tttagagata tagaaaataa tcgaaatttg attttcatag tcattaaaaa
     7081 tgcaacttcc gccatgctta tttattgttt ctcttgcaat gttagaaaca gtatttctct
     7141 taatggaaat agctctgaca ctatttttgt tacctgctgt atagaagctc tcgtcctcta
     7201 tagaaatacg tattacatag aacatatatt cattaatatt tatgcctaac tcccaagcct
     7261 ttttttgtat aaaattattc ccggtaatta taccctttgc aatattttta agaaaatcat
     7321 cttttagtaa ggaaataccc ttgtcaaaaa gagagctttt actggtttcc tcagaaagct
     7381 ttgtatagac ttttgtaaga gattgattta attcctgcgt atttaaaggt tttagtatgt
     7441 aatcacaaac accgtaggct atggccttct ttgcgtactc aaaatcatca tacccgctta
     7501 atacaataaa ttttatgctt tgatcaatct ttagtgcatg ttccataagt tccaagccat
     7561 ccataacagg catttttatg tcggtaatta ctaaatgagg actgtacttt tttataagtt
     7621 caagagcctc ttttccatta ccagcagttc ctaatatatt aaacccggct tctgaccaat
     7681 cgaaatagtt gcaaagacca ttccttattt gtttttcatc ttcggctata atgcatttat
     7741 acactcatac caacccttta cagttcatta tatttacagt aacaattatt tcatattttt
     7801 ttgtttgtgt aaacgttctt acaatagcac aacaactact tattatctgt tttaagtgtt
     7861 atttgcgtaa aatctatatt tatcatagta ataatgatct aaatttattg tgacctattt
     7921 gaactctttc atgacttaag tcatgagttt cctctataat actataaaag ctagaatgat
     7981 gtacacttac catagtaccg ttatagtgca gcacatatag ttcagcacag cgcgccaaat
     8041 tatatatcaa caccagcatt gtataaactc ttcgtaaagt atttgactta tatagattgt
     8101 aacatatttg aaatattgaa aaggaacttt ttttgctcta aaagtgtttt tttttgcatc
     8161 ctgtttaata aggttctgca agttttcttg ttcaccccat tctaacttgg caggcagaaa
     8221 ccggataatt tgtcacgctt tccttttgta atttcaaatc tcctcaacaa aataatatgg
     8281 atctttcctc ataatttcaa gtgccttctt ccataacgca ctggcatcaa taagatacgc
     8341 atttgcatca agtatcttcc ctgattttat ttcatcaccg tcaagcatgc tctcaaccct
     8401 gtcataacgt tgttccagct tgtgatcatt aaaataatgg agcttgcttg tcataaatgt
     8461 cacatgttca tcaggatgaa taatacgata tacacgagta tccttaaaaa ggtatggggc
     8521 ctctgctaat tcctctactc catgcattga tgtattcggc cttagtccac aaccaagaaa
     8581 aaggatttta cctccatatt caggcagttt tcgaaatgga gaatttggtc cgcacggagt
     8641 agtatcaagt atatgatctt ttaagatttc atctgcacaa aaacctgtac cacaaaccga
     8701 gtgagttgga tggatactcc gtattgtacc ttgtctctcc cggaaatatt ccggaaatgc
     8761 ccctacattt gaaggtgtat ttaatacatc aaaaacaggg ttatcagggt ctgcattaac
     8821 atacttatag cttaaagcag ggataaggag agtcccctta tccccaagag catccaatat
     8881 accgctgata acagtttcag ggccgcctgg aacgtatcct aaagatttaa gagaagagtg
     8941 tactaaaata atagctcctt cttttactcc tagttcattt aaagaaacag aaatattttt
     9001 ttggcattca gctttacttg aattcatttt catatcccct attttcctaa tcttttatct
     9061 cctatctctt aactctcatc ccttattcct tatattctta actcttatcc cttattcttt
     9121 atatttgctt cattgaactc tattttctaa ccctttaatc ctttcaacaa tcttatcata
     9181 catctcttga tatttagtct tcttggcttt ttcctcttcc acaacttttt taggtgcttt
     9241 ggaaacaaaa ccagggttgt tcaatttgcc attaaccctt tccagttcct tttcaaggtt
     9301 ggctttttcc ttttcaagtc tttcaatttc cttatcgata tcaataagtt cctcaagggg
     9361 aatataaatc tcagcaccgg gtataaccgc actggcggca tttacagata tactggtttt
     9421 atccgattga attgttgttc ctgatgccca tgccagcttt tcaatgaaat tggagccttc
     9481 ttcgattatc tcctttttat ccttctcctg ggttacaaat ataacttttg ctctctttga
     9541 aggtggtaca tttagttccg accttatatt tcttatgctc cttattgctt ccataataaa
     9601 gcccattctt tcttcatcct gcgggaagtt gtaatcatca ctgtattctg gccatgggga
     9661 tatcattatg cttggttctt caattataag gtgccgatat atttcatctg aaataaatgg
     9721 cataaaagga tgcaggagct tcatagcaga tttcagtaca tagtttaata catactgtgc
     9781 ttcaagtctt gattcgcttt ccctgtcata aagccttggc ttaaccattt caatatacca
     9841 gtcgcaaaac tcttcccaga taaagtcgta gattttctgc aaagcaattc ccaactcaaa
     9901 cttctcaagg ttatctgtca tttcccttgt tagagcatta attctgctta atatccattt
     9961 atcggccata tcaaatttgc ctggatcaac ctttgaaaag tcaagttctt catcaaagtt
    10021 cataaggaca aatctggcgg cattccatat tttatttgca aagtttcttc ctgcttcaat
    10081 tttctcttct gagaatctta aatcatttcc cggagaaatt ccaataacaa gtgcaaatct
    10141 aagtgcgtca gttccatact ggtctattat ttccagaggg tctatgccgt tccccagaga
    10201 tttgctcatt ttccttccaa gagcatcacg aacaatacca tgtataaata catatttaaa
    10261 tggctctctg cccatatgct ccacgccgga aaaaatcatt ctggcaaccc agaagaaaat
    10321 aatatcataa cctgttacca gtacatctgt tggatagaaa tatctcaaat cttcagtttg
    10381 ctcaggccac ccaagggtag agaaaggcca cagtgcagaa ctgaaccagg tatccagagt
    10441 atccgggtcc tgctcaatat tgctgctgcc gcattttgta cacttttcag gtgcttctct
    10501 ttcaaccatc atctctccgc aatctcggca atagtatgca ggaattctat gtccccacca
    10561 aagctgcctt gatatacacc aatcttgtat attttccatc cagttataat atattttcga
    10621 gaacctttca ggaacaaatt tgatggttcc atttttaacc acttctatgg ccggctcagc
    10681 caatggcttc atttttacaa accattgttt tgatataagg ggctctatta ctgttttgca
    10741 gcggtagcac tcacctacat tatgagtatg atcttcaatt tttaataata aatcaagctg
    10801 ctttaaatcc tcaactacct gttttcttgc ctcatacctg tccatcccgc tatactggcc
    10861 tgcgttttcg ctcattgttg catcatcatt tattacctta atctgtggaa gattgtggcg
    10921 aatacctgcc tcaaagtcgt ttggatcatg agcaggagtt atttttacag caccagtacc
    10981 aaattccctg tcaacaaaat cgtctgcaat aataggaatt tctctgttta caagaggaag
    11041 tacaactgtc ctgcctacaa gatgcttgta tctgtcatca tcagggtgta ctgcaacagc
    11101 agtatctcca agcatggttt cgggtctggt ggtagcaatt acaatgtact cattgctgtc
    11161 ttttattgga tatttaatgt gccagaaatg gccttccttt tcctcatact ctacttctgc
    11221 atcagatatt gaagtattgc attttggaca ccagtttata attctttctc cgcgataaat
    11281 caagtctttt tcatatagtc ttacgaaaac ttcctttacc gcctcagaaa ggcctttatc
    11341 catggtgaac ctttcccttt tccaatcgca ggaactaccc agtttcttca attgttcaac
    11401 tattcttcct ccatagtgtt ttctccattc ccatgctctt tcaagaaatt tctccctgcc
    11461 cagcatttcc ttggaaatgc cttcttttgc catagcttca actattttgg cttcagttgc
    11521 aatactggca tgatctgttc ccggaagcca gagagcacaa taaccctgca ttcttttcca
    11581 cctgataaga atatcctgca atgtattatc aagagcatgc cccatatgaa gctgcccggt
    11641 aatattcggc ggtggaatca ctatagtgaa gggcttttta ttatggtcaa ttacagcatg
    11701 gaagtaatct ttttctatcc attcattata aagccggtct tcaacctgtt ttggttcata
    11761 tgttttttca agattttttg cttttatcat gttacggaac ctcctgtcaa tttgtttaaa
    11821 atctattaat tatttaaaat tagttattag ctattagcta ttagttatta gttattagct
    11881 gttagttatt agctattagc tattaattat tggtaatact ttgttatatt gccaatggaa
    11941 agcgcatttc tcgagggtaa tttttgctga cccacctggt tttattgcaa tcaatataaa
    12001 agcccttcat cctaaattaa ttggacgaaa ggctatgtct tccgcgttac caccaaactt
    12061 ccctctagag cattttttta tagcatttat cttattgcag ctgcttctat tccagtttat
    12121 cccactaatt cccatatgat gagagtagtt atgctacctt attaatgaca taattaatga
    12181 aatactaagt gatatactct aaaggaca
//
LOCUS       DAOSDD010000130        12162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2023_length_12162_cov_4.982407, whole genome shotgun sequence.
ACCESSION   DAOSDD010000130 DAOSDD010000000
VERSION     DAOSDD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2023_length_12162_cov_4.982407"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PK733_11820"
     CDS             complement(<1..1343)
                     /locus_tag="PK733_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11265.1"
                     /translation="MKKEQCTFLMATDKHIHYRPSTQRFVSAAVQHPGNPHIIPEAPW
                     EVHIGYNSQYRNPNTGCYQLWYQAFSGKKANERTHQCVVCYAESVDGVNWIKPNLGLY
                     SYNGIAETNIVLLGNGGFSCRYGAGVIVDENSKEPEKKYKMAYFDWNMSGGKEFPGLC
                     IAFSADGIKWNKYPHAPVLISSYGSRGDETPLRYERGKEWLVPLSISDAVDPFYDFEK
                     ECWAIYGKMWIDGPDGRMFWKHAMGRTESIDFIQWSKPELILTPDEYDDDYLEFHASP
                     VFYYEGLYFSLIQVLNRKNNGGTLEIELAVSDDGLKWERPFRRQPFISKSGKLFDCGS
                     ILTNSTPIILDDEIRIYFGGVSGGVVSGDDYNMATGIGYAVIKKDRFAGIKPINNIGQ
                     ITLKSIDFDGYSNIYINADASSGKVAGEILDEDGYRIPGFTREDAIPLQNVDNTR"
     gene            complement(1426..3093)
                     /locus_tag="PK733_11825"
     CDS             complement(1426..3093)
                     /locus_tag="PK733_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11266.1"
                     /translation="MKKLLASMLILTMAFSLLAGCVQKADNKGGTSITQKEGTKTDAD
                     LKDTKGTGNTGEPAEVSFALSYSMDYNNYSMPSQKTDWPILKEMEKNTNVKIKWIMYP
                     GSEYATTMNTQLASGSDLPDMLILPKGAKADILLQNGILIDLMPYLKTDGLDMIKAMQ
                     KYPEIIVANTLDDGRMHSFPSTITTGKENLPSICLREDWMTQLSLKVPVTIDDFTGVL
                     QAFKNGDANKNGKADEVPLTGRYWHFIQYLRPGFGIYDKIAYPGADGTFEIELLKPGY
                     KDYIAWVANLYKNNLIDHKMFEPGDQQARVVELLKSEDSTLGGVIWGSNSVCGGFTKT
                     YLKPVNENAQLTVVLPPISQYVSKIEIQRKNALAGGFAITKYCKNPNAAIRYFNYGFS
                     EEGRILVNWGIEGDSYKVVNGEKIMQEKMFNTKEDDEAGWYRAEAYGMHSFGTYEDWD
                     AWAQVIKWDERDKEWNNSLKNIATKLPFPLLVYNDSERILRDRIVTDLESFVDENTLK
                     FITGTRPIEQWDEFVKELTEKYKIKDLIDGVYNPAYKRFNEKIDNFK"
     gene            complement(3176..4090)
                     /locus_tag="PK733_11830"
     CDS             complement(3176..4090)
                     /locus_tag="PK733_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11267.1"
                     /translation="MEQRIKYKRSTGDIFYDTIIYLIIFFAIIITLYPFIHVLSLSVS
                     NFDQVLKGKVYLLPKGFTLDAYKIVVSEKGIWRAYLNTIVYTSISTVFVVTFTFMAAF
                     PLTRKDYIFKKIMTSLFLIPMFISGGLIPSFILIKKLHLYDTMWSVILPGIISTWNIV
                     LVRTYIQGNIHENLLEAARIDGMNDIGILFKIVVPLCKPILAVIALYTIVGHWNAYFN
                     VMIYIPSREKQTLQLYLMRVLLRNSDILSNAQNQLASAHGNESMSMLAGMQLKYALII
                     FVIAPIIMVYPFLQKYFIQGVMIGALKG"
     gene            complement(4128..5042)
                     /locus_tag="PK733_11835"
     CDS             complement(4128..5042)
                     /locus_tag="PK733_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11268.1"
                     /translation="MIPKSTLIKLSRDKYLIMLSVIPVALLIIFSYIPMYGIVIAFQD
                     YFPGQPFFSLEKWVGLSQFQRFFESPLAWRLIRNTFLLNIYSIFAGFPIPIIFALCLN
                     ELYSPRLRKVAQTISYLPHFISTVVICGLIVNMFSTEGGIINNFILSITGKKINFFMD
                     PKYFRHIYVWSGIWQTFGWSSILYLSAIASIDPTLYQVAEIDGASRMQRTKYITMPGI
                     LPTISIVLILNFASLFSIGSEKVILLYNPAIYETADVISTYVYRTGLIEAQYSFGTAI
                     SLMNAIISLVLLFTFNSIAKKIKMATFL"
     gene            complement(5188..6915)
                     /locus_tag="PK733_11840"
     CDS             complement(5188..6915)
                     /locus_tag="PK733_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11269.1"
                     /translation="MTDDEFHIREGLAKSIKWDNMGFGVCACASNGLEALEAIEKENP
                     DVVVIDIKMPTMDGIELCKILYETKPRIKKIILSGYEDFEYAQKAIEYNVNAYLLKPL
                     NEKKLFEELRKLKTLLDSEKAEEMGKINNRILLENNIKMHWQLLGFKLINSNFIPDAN
                     LLGFADGSILNISIENTCIIAATIQNEKSGVYLTDKYLNACMDYWTKMNYPVFLDNNI
                     LVILPNSNTKLFQKQIIYEANEFKEWIDLNIESVTLNAGIGSFVPFKDINKSKTEAYY
                     TLDYTFFKNKGTILTVSDIRSDISNIILKAFKKDRKLFDFDINIDIDKDIDKDIEKDI
                     DKDIDKDKDRIKSKLHEAFILAAEGFIDKMFEEKFKNFTIDLKHFFDEILLAGINNRL
                     TVFLRCVEVYILFVKRINDRKFSIEYITTDELYTKISRSINSTELLILFNDILLNMVN
                     QILAIINDEDSFIIQKIKAFIKSNFNTDLSLNKLAEEFFLNPSYLSTYFKKETGITIS
                     EYITKVRVNNSKELLKNLDLPIYNIAEKVGYSDYRYFCTVFKKLCGVSPLQYRLNELK
                     YGEIPENDI"
     gene            complement(6956..9697)
                     /locus_tag="PK733_11845"
     CDS             complement(6956..9697)
                     /locus_tag="PK733_11845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11270.1"
                     /translation="MIGSLESLIKKPRFTFRRMLISISIVFCTIILSISSYAFFMSIQ
                     DFIVSKFSDDYFNYIYLVEKEFTDTFSKLEKIASTISKNKLLLDNINNYERDISDKIH
                     TQKEISSSLTKFKNYNDNILMIYFLTENNNFFISTEPTSCTFKDLKQYGFAGLLKDKM
                     KFLEAGSEDYRLLRNLLKINVDSIVCQYPIFNNDIEEGQIIFVLRKDIFSRTLQKYLN
                     IALVDNKNNVIFNNTNISNMDILKNEDFRSANNKYMYKDKNENIPYLSQISITGNQYK
                     IFYFHQNTNIKIAVKNLKKYFFASLMTLIIVTVITSYFLAKIIVIPINNLSEAVDEYT
                     LKEKANSIPDSIPPKLTLREHIMYYFMIIALMPLFMFFASSFYYIKNQLSEYLIELNQ
                     SSFNNISSNLDTYFDNKKRILNYIAYDSNIQGALSGGINQSYNEKLVKVIDNYQLLDI
                     GHDDIYIYSLNLSPIISNTYDHKYPDIDISHINNSKRSNIFSFWLEPETDEYGRTYLA
                     YIMTINDINTYNVSGYLLCKTYETQIEKVYTVPSVNTQNTYIIDNGGKVISTFNKKLL
                     GSNYQKSISKIFTYDPLEKEIFKKNISDTEWSLIGFYDYKRIDESIMQIVYKQLYIVA
                     ILALFAVILSYIISIRFTSVIEKFNTLLRKMGISNIDIVFPEDTSINEIYRLAASFNN
                     MVLRIETLVDQLILSSTKQIQLEARKKNAEMLALQSQINPHFIYNTFESIQWLIKSKS
                     NEKAILMLDSFSNMLRYSTASKSMMITIEEEISHLNNYINIMDVRYNGRIKFICEIVS
                     SILQYKTIKLILQPIVENAIYHGIRAEQEDGIIKISCLEEENELIFTISDNGKGIDLI
                     ALEEINKCLSSNAYPDRIGIFNVQTRIKLFFGDDYGITIDSKYGKGTIVKVRIPKSI"
     gene            complement(9771..10148)
                     /locus_tag="PK733_11850"
     CDS             complement(9771..10148)
                     /locus_tag="PK733_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11271.1"
                     /translation="MVYPSVTWCPDIGKYRLWYEALPDMSVDAVRYLAVAESSDGINW
                     FPVEVQDKADEICARYKNVVYSGHGGIHGTSVYRDEHEKNPQWRYKATGMTRTDQMKK
                     VPKGEIVCPQKSISDPIQLGVDR"
     gene            complement(10490..10687)
                     /locus_tag="PK733_11855"
     CDS             complement(10490..10687)
                     /locus_tag="PK733_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11272.1"
                     /translation="MRRLVMEDADMKEMTGLGHEDPLKVLTPEAIKHATVIVTTIPIV
                     LLYPLLQKYFVKGIMIGAVKG"
     gene            complement(10710..10973)
                     /locus_tag="PK733_11860"
     CDS             complement(10710..10973)
                     /locus_tag="PK733_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11273.1"
                     /translation="MKITLVPKTALGKWSVILSIAFIVFIALKTQSFFPLPSFSIAAL
                     GLAGFVVGIIAIIKKDRALLFFIPILVGLVIILWIAGELIYPH"
     gene            complement(11245..11451)
                     /locus_tag="PK733_11865"
     CDS             complement(11245..11451)
                     /locus_tag="PK733_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOJ11274.1"
                     /translation="MNKKSRKELLEEYKQLKTYMGVIQIKNNASGKIHIAAYPNLKNK
                     WLTIQGQLNMGMHVNSQLQKDWNT"
     gene            complement(11605..11927)
                     /locus_tag="PK733_11870"
                     /pseudo
     CDS             complement(11605..11927)
                     /locus_tag="PK733_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
BASE COUNT         3880 a         2256 c         1612 g         4414 t
ORIGIN      
        1 caccttgtat tatcaacatt ttgcaatgga atggcatctt cccttgtgaa tcccggaata
       61 cgataaccat cctcatcaag tatttctccc gcaacctttc ctgaggaagc atcggcattt
      121 atgtaaatat ttgaatatcc atcaaaatct atgctcttta atgtaatctg tccaatattg
      181 tttataggtt ttatccctgc aaatctatct ttctttataa ccgcatatcc tatacccgtt
      241 gccatattat agtcatcgcc gcttaccaca ccgcctgaca caccgccgaa atatattcta
      301 atctcatcat caagtataat tggtgtggaa tttgtcaata ttgaaccaca atcaaacaat
      361 tttccacttt tagatataaa tggttgtctc ctgaatgggc gctcccattt taaaccatca
      421 tcacttactg caagttcaat ttctaatgta ccgccattgt tctttctatt aagaacctga
      481 attaatgaaa aataaagccc ttcataataa aacaccggcg aagcatgaaa ttccaagtaa
      541 tcatcatcat attcatcagg tgtaagtatt agctcaggtt ttgaccattg tataaaatct
      601 atactttctg ttcttcccat ggcatgtttc cagaacattc tgccatccgg accatcaatc
      661 cacattttgc catatattgc ccagcactct ttttcgaaat cataaaacgg atctacagca
      721 tccgaaattg aaaggggtac cagccattcc ttccctcttt catatctaag tggagtttca
      781 tctcctcttg aaccataact cgatataaga acaggagcat gaggatactt attccatttt
      841 attccgtcag cagagaaagc aatacaaagt ccgggaaact cttttcctcc tgacatattc
      901 cagtcgaaat atgccatttt atatttcttt tcaggctcct ttgaattttc atcaacaatt
      961 actcctgcgc catatctgca agaaaaacct ccgtttccca acaatacaat attagtctct
     1021 gctatgccat tgtaagaata gagaccgaga tttggtttta tccaattcac tccgtctaca
     1081 gattctgcat aacatacaac acactggtgt gtacgttcat tggctttttt cccggaaaat
     1141 gcctgatacc ataactggta gcaacctgta ttgggattcc gatattggct gttgtatccg
     1201 atatgcactt cccagggggc ttccggaata atatgagggt tgccgggatg ctgcacggca
     1261 gctgaaacaa atctttgagt tgacggtcgg tagtgtatat gtttgtcagt tgccataaga
     1321 aaagtacatt gttctttttt caaaattgtt acctccacac aaaattttgg caggaataat
     1381 tttctaaagt attagcaatt attcctgcca ggcaagcaca tgagtttact taaaattgtc
     1441 tattttttca ttaaatctct tgtatgccgg attatagact ccatcaataa gatccttaat
     1501 tttatatttt tcggtaagct cttttacaaa ctcatcccac tgttcaatag gccttgttcc
     1561 tgttataaat tttagtgtat tttcatctac aaaagattcc aggtcggtca ctattctgtc
     1621 tcttaaaatt ctttcactat cattataaac tagtaagggg aagggtaatt ttgttgcaat
     1681 atttttcaag ctattattcc actctttatc cctttcatcc catttaataa cctgagccca
     1741 tgcatcccaa tcttcatatg ttccaaatga atgcatgcca taagcctcag ccctatacca
     1801 gcctgcttca tcatcttctt ttgtattaaa cattttttcc tgcattattt tttcaccatt
     1861 aaccacttta tatgaatccc cttcaatacc ccaattaaca aggattcttc cttcttcact
     1921 aaagccataa ttaaaatacc ttattgctgc atttggattc ttgcaatatt ttgttatagc
     1981 aaaacctcct gctaaagcat ttttcctttg tatctcaatt ttgctgacat attgagaaat
     2041 tgggggtaat actaccgtaa gctgcgcatt ctcattaacc ggtttgagat aagtctttgt
     2101 aaaacctcca catacgctat tcgaacccca tattacacct ccaagagtag agtcttcact
     2161 ttttaacagt tcaacaactc ttgcttgctg gtcacctggt tcaaacattt tatgatcaat
     2221 cagattattc ttatataagt ttgctaccca tgctatataa tctttatatc ctggttttaa
     2281 aagctcaatt tcaaaagttc cgtcagctcc tggatatgca attttatcat atattccaaa
     2341 gcctggcctt aggtattgaa taaagtgcca gtatcttcct gttaacggaa cctcatcggc
     2401 ctttccattt ttatttgcat caccattttt aaaagcctgc aaaacaccgg taaaatcgtc
     2461 aatggtaaca ggcaccttta aggataactg agtcatccaa tcttctctta agcaaattga
     2521 tggcagattc tccttacctg ttgtaatagt agaagggaag ctatgcattc tcccatcatc
     2581 aagagtattc gcaacaatga tttcgggata tttctgcatt gcttttatca tgtccagtcc
     2641 atctgtttta agatatggca taagatctat caatatgcca ttctgaagca atatatcggc
     2701 ttttgcgccc ttgggtaata ttaacatgtc agggagatcg cttccagacg caagttgtgt
     2761 attcatggtt gttgcatatt cactgccagg gtacattatc catttgattt taacatttgt
     2821 gttcttttcc atttctttta atataggcca atcagttttt tggctgggca tagaataatt
     2881 gttataatcc attgaatagc ttagtgcaaa agacacctct gcaggttctc cggtattgcc
     2941 ggtccctttt gtatctttta aatctgcatc tgttttagtg ccttcttttt gcgtaatact
     3001 tgtgccacct ttgttatctg ctttctgaac acaacctgca agaagagaaa acgccatggt
     3061 taaaattaac atacttgcca gtaatttttt cattttaaca ctcctccttg tataatattt
     3121 tatatataat aatctggtac tccatttaat taataaagca taaatataga ccgattcagc
     3181 ctttaagggc tcctatcata acgccttgaa taaaatattt ctgcaaaaat ggatatacca
     3241 taattattgg ggctataaca aaaattataa gggcatattt aagctgcatt ccggctaaca
     3301 tagacatgct ttcatttcca tgagcagaag caagctgatt ttgtgcattt gataaaatgt
     3361 ctgaattcct cagaagtacc ctcattaaat aaagctgaag agtttgcttt tctctgcttg
     3421 gaatatatat cataacatta aagtatgcat tccaatggcc cacaattgtg tatagggcaa
     3481 taacagcaag tatcggcttg caaagcggaa caactatctt aaataaaatg cctatatcat
     3541 tcattccatc tatcctggct gcctcaagaa gattttcatg aatattgcct tgtatatatg
     3601 ttcttactaa aacaatattc catgttgaaa taattccggg aagtattacc gaccacatag
     3661 tatcatataa atgaagtttt tttatcaaga taaatgaggg aatcaatcca ccgctaataa
     3721 acatgggtat caaaaacagg ctggtcatta tttttttaaa aatataatcc ttccttgtaa
     3781 gtggaaatgc tgccataaaa gtgaatgtta ctacaaaaac tgtagatatt gaagtatata
     3841 caattgtatt taaatatgca cgccaaatac ctttctcact taccacgatt ttataagcat
     3901 ctaaagtaaa accttttggc aaaagataaa ccttgccttt aagcacctga tcaaagtttg
     3961 atactgataa gcttaaaaca tgtataaaag gatataaggt aattattatt gcgaaaaata
     4021 taataagata tattatcgta tcataaaaaa tatcaccggt acttctttta tatttaattc
     4081 tctgttccat tatacatatg acctcccata taagtttttt atacttatca taaaaaggtt
     4141 gccattttaa ttttcttagc tatactgtta aatgtaaata gcaacacaag gctaatgatt
     4201 gcattcatca ggctaattgc agttccaaag ctgtattggg cttcaatcag gcctgttctg
     4261 tatacataag ttgatattac atctgctgtc tcataaatag caggattata caaaagaata
     4321 accttttccg aacctatgga gaaaaggctt gcaaagttga gtatcaatac aatacttatt
     4381 gtgggaagta tacccggcat agttatatat tttgttcttt gcattctgga agccccatct
     4441 atttctgcca cttggtataa cgtagggtct atacttgcta ttgcggatag gtacaatatg
     4501 gatgaccacc caaaagtctg ccaaattcca gaccaaacat aaatatgcct gaaatattta
     4561 ggatccataa agaaattaat ttttttacct gttatcgata atataaagtt attaattatt
     4621 cctccttctg ttgaaaacat atttacaata agtccacata taacaacagt ggatatgaag
     4681 tgaggaaggt aacttattgt ttgcgcaact ttccttaatc tgggactgta taattcattt
     4741 aagcataatg caaatattat aggaattggg aatccggcaa aaatggaata tatatttaat
     4801 aaaaatgtat ttctaataag cctccacgct aaagggcttt caaaaaaacg ctgaaattgt
     4861 gataaaccca cccatttttc caaactaaaa aacggctgcc cggggaaata atcttgaaag
     4921 gcaattacaa taccatacat gggtatatag ctaaatatta taagtaatgc aacaggaata
     4981 acacttaaca taatcaaata tttatctctt gataacttaa ttaaagtaga ttttggtatc
     5041 acaattattt ccctctcact tcagtaataa ttttcttgtt atatctaaat tatatataaa
     5101 ttatatacta gctggtcaat gaatactgtt taataattct agaattcgtt taattttttt
     5161 gtttatatga aagtaaaacc agatttttca gatatcattc tccggaatct caccatactt
     5221 caattcattc aatctgtatt gcaacggaga caccccgcac aattttttaa aaactgtgca
     5281 aaaatatcta tagtccgaat atccgacctt ttctgctata ttatatatag gcaaatcaag
     5341 attctttaat agttcttttg aattattaac tctaacttta gttatatatt cagaaatggt
     5401 aattcctgtt tctttcttaa aatatgtact taaatatgaa gggttaagaa aaaattcctc
     5461 tgccaattta ttaagactta agtcagtatt aaaattggac ttaataaatg cctttatttt
     5521 ctgtattatg aaactatctt catcattaat aattgctaaa atttgattta ccatatttag
     5581 caatatatca ttgaataata ttagtagttc tgtagaatta atagaacgtg aaatcttagt
     5641 atataattca tcagttgtta tatactctat actaaacttt ctatcgttta ttcttttcac
     5701 aaaaagaata tatacttcaa cacatcttaa aaaaacagta agtctattgt taataccagc
     5761 taataatatt tcatcaaaaa aatgttttag atcaatagta aagtttttaa atttctcctc
     5821 aaacatttta tcaataaatc cttctgctgc caggataaaa gcttcatgaa gctttgattt
     5881 aatcctatcc ttatccttgt ctatatcttt atctatatct ttttctatat ctttatctat
     5941 atctttatct atatctatat ttatatcgaa atcgaataat ttcctgtctt ttttaaacgc
     6001 ttttagtatg atattactta tatcacttct tatatcactt acagttaata ttgttccctt
     6061 gtttttaaaa aacgtataat ctaatgtgta atatgcttca gtttttgatt tattaatatc
     6121 tttaaacgga acaaaagatc ctattcctgc atttagggtt acactttcaa tatttaaatc
     6181 tatccattct ttaaactcat tagcttcata aataatttgc ttctgaaaca gcttagtatt
     6241 actgttaggc aaaattacta aaatattgtt atcaaggaaa accggataat tcatctttgt
     6301 ccagtaatcc atacacgcat tcaagtattt gtcagttaaa tacacaccac ttttttcatt
     6361 ttgaatagtg gctgcaatta tgcatgtatt ttctatagat atattaagta ttgaaccatc
     6421 agcaaagcca agcaaattcg catcaggtat aaagttgctg tttataagct taaaccccaa
     6481 taattgccaa tgcattttta tgttattctc aagcaatata cgattgttga tttttcccat
     6541 ttcctcagct ttttccgaat ccagcaatgt tttaagtttt cttaattctt caaataattt
     6601 tttttcattt aggggtttta ataagtatgc atttacattg tattcaatag ctttttgtgc
     6661 atattcaaaa tcttcataac cactcaaaat tattttttta atacgaggct ttgtttcata
     6721 aagtatttta cagagctcaa taccatccat tgttggcatt tttatgtcaa taactactac
     6781 atcagggttt tctttttcaa tagcttctaa tgcttccaat ccatttgaag cacatgcaca
     6841 aactccaaaa cccatattgt cccacttaat agatttcgcg agaccttctc ttatgtgaaa
     6901 ttcatcatct gtaattacaa ccttataaat aaaatcatcc ccataaaaat aattactata
     6961 tgctcttagg aattcttact ttaactattg tacctttccc atacttactg tcaatggtta
     7021 ttccataatc atccccaaaa aaaagtttga ttcttgtctg tacattaaat atacctattc
     7081 tatcaggata tgcattgctt gaaagacatt tgttaatttc ctctaaagcg attaagtcta
     7141 tacctttacc attatcagaa atagtaaata ttaattcatt ttcttcctct aaacatgaaa
     7201 tttttataat accatcttct tgttccgccc ttataccatg ataaattgca ttttcaacta
     7261 taggttgaag tatcaattta attgttttat attgcagaat tgaagaaaca atttcgcata
     7321 taaatttaat tctgccatta taacgaacat ccattatatt aatataatta ttaagatgag
     7381 atatttcctc ttcaatcgtt atcatcatgc tttttgatgc tgtactatat cttaacatat
     7441 tgctaaatga atcaagcatt aaaatcgctt tttcattact tttcgatttg attaaccatt
     7501 gaatactttc aaaagtgtta tatatgaaat gcgggttaat ctgtgattga agcgccagca
     7561 tttcagcatt ttttttcctt gcttccagct gtatttgctt tgtagaagat aaaatcaact
     7621 ggtcaactaa tgtttcgatt ctcagaacca tattgttgaa ggaagccgct aacctatata
     7681 tctcattaat gctggtatcc tccggaaaca caatatcaat attgcttatt cccatttttc
     7741 ttaaaagagt attgaatttt tcaataactg atgtaaacct aattgatata atatatgaca
     7801 gaataactgc aaataaagct aaaatagcta ctatatatag ctgcttatat acaatttgca
     7861 taatactctc atctattctt ttataatcat aaaaacctat caatgaccat tcagtatcac
     7921 ttatattttt cttaaatatc tccttttcta atggatcata agtaaagatt ttacttatag
     7981 acttttggta gtttgaacct aaaagttttt tattgaaagt tgaaattact tttccgccat
     8041 tatcaataat atatgtattt tgtgtgttta cagatggaac tgtatatact ttttcaattt
     8101 gagtctcata tgttttacaa agcaaatatc cagaaacatt ataagtgttt atatcattaa
     8161 tagtcataat gtaagcaaga taagttcttc catattcatc agtctccggt tccagccaga
     8221 aagaaaatat attacttctc tttgaattat taatatggct aatatctata tcaggatatt
     8281 tatgatcata tgtattagaa attattggcg ataagttcaa tgaatatatg tatatatcat
     8341 catgacctat atctaaaagc tgataattat caattacctt tactaacttt tcattataag
     8401 attgatttat accgccggat agtgctcctt gtatgtttga atcgtatgca atataattca
     8461 gaattctctt tttattatca aaatatgtgt caagattaga agaaatatta ttaaaactgg
     8521 attgattcaa ttcaattaaa tactcactta attgattttt tatataataa aatgaactgg
     8581 caaaaaacat aaatagcggc attaaagcaa taatcatgaa gtaatacatt atatgctccc
     8641 gcaatgttaa tttaggcggt atggaatccg gtatgctgtt ggctttttct ttcaatgtat
     8701 attcatccac tgcttcagac aaattattaa tcggtatgac aataatcttt gcaagaaaat
     8761 atgaagttat tacggtaaca ataatgagag tcataaggga cgcaaaaaaa tattttttta
     8821 agtttttaac tgcaatttta atattagtat tttgatgaaa gtaaaatatt ttatattggt
     8881 ttccggtaat actaatctgt gataaatatg gaatattttc gtttttatcc ttatacatat
     8941 atttattatt ggcacttcgg aaatcctcat tctttaaaat atccatattt gatatatttg
     9001 tattattaaa aataacatta tttttattat caacaagcgc tatattcaag tatttttgca
     9061 atgtcctgct gaatatatct tttctcaata caaatatgat ctgtccttct tcaatatcat
     9121 tattaaatat tggatattgg catacaatag agtccacatt tatttttaga aggtttctta
     9181 agagcctgta atcttctgaa ccggcttcta gaaacttcat tttgtctttt agcaatcctg
     9241 caaaaccata ttgctttaag tctttgaaag tgcaggaagt gggttccgta ctgatgaaga
     9301 agttattgtt ttccgttaaa aagtatatca ttaatatatt atcattataa tttttaaact
     9361 ttgtaaggct tgatgaaatt tccttttgag tatgaatttt atcagatatg tctctttcat
     9421 aattatttat attatctaaa agcagcttgt ttttgctaat agttgaagca attttctcta
     9481 attttgagaa agtatcggta aattcttttt caacaagata aatataatta aaataatcat
     9541 cagaaaattt agacactata aaatcctgta tactcataaa gaaggcatag gatgatattg
     9601 aaagtataat cgtgcaaaaa actattgata tacttattag cattcttcta aatgtaaatc
     9661 ttggtttttt gattaagctt tcaagacttc caatcattta aattcctcct aattttgcat
     9721 atgccttgct tttcgttttt atttaggtgc acatttatat ttttctcctt ttacctatct
     9781 acacccaact gtataggatc cgatattgat ttctgagggc atacaatttc tcctttagga
     9841 acttttttca tttgatctgt tcttgtcata ccagtcgctt tatatctcca ttggggattt
     9901 ttttcatgct catccctgta aacagatgtc ccatgaattc cgccatgacc gctgtataca
     9961 acatttttat aacgggcaca tatttcatca gccttatctt gaacttctac aggaaaccag
    10021 ttaattccat cactgctttc cgcaacagcc aaatatctta ctgcatccac cgacatatct
    10081 ggcagagctt cataccaaag tctatatttg ccaatgtctg ggcaccatgt aacagatgga
    10141 tacaccacgg cttggactga ctcatcttta tagcttccta taaatttagg cgaataccac
    10201 ctgcgttgta tgtttatctt acaatccagc caaaaatcat caaaaaagct taagcttgtt
    10261 ctttcaatga cacttcctcc cccctagctt aatataaatt tagtaataaa tctctctata
    10321 tattatatca tgaatttttt taaattcatg atataattgt gcatatgcgt tttttgcgaa
    10381 gcaaaaatat tcgcacgcac ataaattccg ccggaggcat ataatatcct catttttgag
    10441 gatattatat cataaatgca atctgccaat cggattgctt cacaagcact caacctttca
    10501 ccgctccaat cataattcct tttacaaaat acttttgaag taatggatat agcaatacaa
    10561 tcggtattgt tgtaactatt actgttgcat gctttattgc ttcaggagtt agaactttca
    10621 atggatcttc gtgacccaat ccggtcattt ctttcatatc cgcatcttcc attacaagtc
    10681 gccgcaaaaa aacctgcaga gtgtattttt tagtgtggat aaattaattc acctgcaatc
    10741 cagagaataa ttaccaatcc caccaggata ggaatgaaaa ataaaagagc ccgatccttt
    10801 ttaatgatgg caattattcc tacgacaaaa ccagccagcc ccaaagcagc aatagaaaaa
    10861 gacggcaatg gaaaaaaact ttgtgttttt aaagcaataa aaacaataaa cgcaatactc
    10921 aatataacag accatttccc taaggcagtt tttggtacta aagttatttt catacgttta
    10981 atcccctttc ttgatatgcc aataatgaat aactcaggca aaactctata ctataactct
    11041 atatgcaact ctatatataa ctctatacta taactccatg atataaaaaa tatcaatata
    11101 ttaaagtaaa tatttacatt tttttattaa taaatatcca aaaaaacaag gctaaatacc
    11161 agctgtcttc caaatttaac atttctctta agcatatcca catgcaccaa ttaacactta
    11221 ttcacttgtc ggcttattat atccttacgt attccagtcc ttttgcagct gggaattcac
    11281 atgcataccc atgttaagct ggccctgtat ggtcaaccat ttgtttttta aattgggata
    11341 agctgctatg tgaattttac cactggcatt gttttttatt tgaattacac ccatgtaggt
    11401 ctttaactgc ttgtattcct ccagcagttc cttgcggctt ttcttgttca taattttcta
    11461 tcctttcagg tttacataat aataacaatt ataattaatt atcgtatcta caattctcct
    11521 atatacaatt ttcctatata ccttatctga tttatcttca caggcattta taataaaggc
    11581 agttcttttt tccctgtgta taaattaata tcccatctct ttcaaaattc ttgataaggt
    11641 gcgcaatcgt tcactaataa gctcatcgtc atcactgaga gcttggttga ataacttaat
    11701 atcttcatca attatttcgt tgtctgtaca tatgattgcc ttgaccactt ttctccagtg
    11761 tatctccaca ctttagctga aatgtctact ttaccacggt cattccattg tgctaatccc
    11821 agtgaaagtc cttttgcatc cgaattatag gcatatctgc catcaacatt ttcatagttt
    11881 tcagaaacaa taaccggctt atgtttcaga ctagttggta ttttaataat ttcatctcct
    11941 cgaatattcg taaatggctg tgaatgtgaa ttcatatgca catatattca tatgaattca
    12001 tgtttatata tacgctatat tgcttcgtaa attactgaat tagttattta ctaagctact
    12061 aaatatacaa tatcatttaa tatagcctct gtcaatgcat attgttattt gagtttcccc
    12121 attttcaata accaaaacag ctatctgcct tgataccaaa ga
//
LOCUS       DAOSDD010000131        12149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2028_length_12149_cov_5.618654, whole genome shotgun sequence.
ACCESSION   DAOSDD010000131 DAOSDD010000000
VERSION     DAOSDD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2028_length_12149_cov_5.618654"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PK733_11875"
     CDS             complement(<1..962)
                     /locus_tag="PK733_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ11275.1"
                     /translation="MFQGSFFTNLLFTKKGSDNIDRENIRSNQKALTLVELVITLAII
                     SFLTLIIIQVFAMNNAMANNQLKESAQRHEIRAAAAYLTEDIKFSKAINFDIAKPNEL
                     IITDKDDQSIIYYIAADAEGNNYLKRQAATETEFKEIKDVSFSLDNSTLVSAKLITDI
                     ANNKFNEFKITSLGTQVALSLPVELTPEEKFRQFVLNESVFIYGSELTFAGNECVFEE
                     GTIFVTDDFSGRINGGAKIKVKHLYTNGDLAVSQGHTLGLVDESNIIFINGTLTFDGG
                     AKMYGDVNVNENLVMNNGTINGNVNVAGNATLSGNPTINGDINV"
     gene            complement(966..1397)
                     /locus_tag="PK733_11880"
     CDS             complement(966..1397)
                     /locus_tag="PK733_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ11276.1"
                     /translation="MFKFINKKGFSIVEVLIAVAIMVVITGIFLNLLFTGYKARNTSR
                     ERLQMLAYSTSIVDEIKAYQYDWKNFAGLKARLLNEGYIETINNAFCKVASDSLGISY
                     DIKLYIEKDKGIDKLIQISIEVKPLNSYVNPIKMSVRLREV"
     gene            complement(1466..3166)
                     /locus_tag="PK733_11885"
     CDS             complement(1466..3166)
                     /locus_tag="PK733_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ11277.1"
                     /translation="MKWLPLTAKNSLLGELLINEGIITKEQLESALEKQKTKEQNKKK
                     GLIGNTLVELGYCTENDISRILARKAGVQFLEMNNYEIDMSAANLITPELAKRYNVLP
                     IGFENDRLLLAMKNPGDIIAIDDLRIITGCSIKPVVINDKELETAIKRFINMSMALQE
                     TDEIEEIEEDETENDDLNEKPAVQLANQIINQAVKAGASDIHIEPQEKRLRIRYRIDG
                     VLHEIMQQQINLHPTLVSRLKVMSNMDIAERRIPQDGRITLKVDGNIIDVRVASMPSA
                     YGEKITMRLLNRSDRLITLKELGFNEYELEKYEITTKSPYGFILITGPTGSGKSTTLY
                     ASLAKLNQPDRNIITLEDPIERRMDGLTQVQANMKAGMTFASGLRAILRNDPDIIMVG
                     EIRDHETARIAVESALTGHLVLSTMHTNDSAGAVTRLSDMGIEPYLTASSLIGVVAQR
                     LMRVLCKECKQPYIVTRQQLINSIPDFPFEGDEEEIQLFKSKGCVYCNGTGYRGRVGI
                     YEFLKVTESIQKLMLSRASAKEIRQAAIREGMLIMKQDGLQKAKKGITTVEEVLRVIV
                     "
     gene            complement(3179..3700)
                     /gene="pilO"
                     /locus_tag="PK733_11890"
     CDS             complement(3179..3700)
                     /gene="pilO"
                     /locus_tag="PK733_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOJ11278.1"
                     /translation="MYKRKLSIPVIVLCICILIASFLLIREIIILKGNLDNISMVNNE
                     IDGLNEKLIYLKGYEQRADEMLGMLDKYRRALPETPEEGNIILGLSKLASENSIELGE
                     IRFDEGTQEETLAKLPFHVNASGEYFDLVKFIVGIKDNERIFVIREVNIERNDDSQKL
                     IADINIVAYNIRK"
     gene            complement(3710..4288)
                     /locus_tag="PK733_11895"
     CDS             complement(3710..4288)
                     /locus_tag="PK733_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOJ11279.1"
                     /translation="MSKISLMPLEYKNLIIRNKKKRLFSMIGVIVVSALIMSYIMLIL
                     INIIYVNQLNSLNIEKNQLESMISTYGKYSDVYNSVKKQEKLIEKANIDSYNWDTLLN
                     TFAETIPQGMWFDSISLDYDGKKGTCVIAGRSTNKVIMAQWLNDIEKKEILKNIACKY
                     LQDYDDGEKKVYEYEINSEIDMSIIKTVKGME"
     gene            complement(4293..5432)
                     /gene="pilM"
                     /locus_tag="PK733_11900"
     CDS             complement(4293..5432)
                     /gene="pilM"
                     /locus_tag="PK733_11900"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOJ11280.1"
                     /translation="MSLYKKSVIGIDISCSQIHFVEMSRSSQTPVIQKTGKVFLDKDV
                     VKEGKVVNQDKLKNVLEELWKKNRIKSKEIILGISNNDIIMRFITLPKMPLDKLSNLI
                     KFQVADYMPVNIDDYELDYTIISENANESGSYYNVLLVAAGKKMLYEFINAFSTVNSS
                     IKDIKSSVLVMDKLVPGEYRNSVSVVVNLSSEACNLLIVNNNLPAFARTILLKENMHR
                     EIDIINKEKFSASWNANAGYSYRDTVPTENTKLVGISNTAVMEKEGLHSFEHSNLQCN
                     QNKLILNIVSFVGDEIRSSISYFQSQNRDSFIEKIFLTGCACSNEAVYEGLADYLGMN
                     VEILQPYENLFSVLSKKNKDKVIVDDYEPSKFAIALSLALHGLED"
     gene            complement(5452..6660)
                     /locus_tag="PK733_11905"
     CDS             complement(5452..6660)
                     /locus_tag="PK733_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ11281.1"
                     /translation="MADYIYECINRQGEIVKGKISADNNSIAIDKLKKMELMIVDLKQ
                     MDTTVRKTSGLFQKKVSIGELAMFSKQMAAMLNAGIPVTRALFTIGQQATNPTLKAAL
                     ITIAQNVEGGRSLTDAFSMYPKIFSDLYISMLHSGEVGGILDDSLTRLADQLQKEKVL
                     IDNIKASTFYPRMVAGFALILFVAMLVFMVPIFKGFIPENAAIPAITAMIFSLSDSIR
                     GSWFIWIAVLAVFVFVIYIFVRSPAGKRIWESIKFKIPAFGPIMHKAVLARFSRTLAT
                     LLEGGIPIIQALEGAGPTSGSLLVNEVINNTVKKIEEGKNISDQLKESYLFPPMMIQM
                     IAVGEETGSLSSMLDKMAEFYEDEVTTLTKGITALIEPIMLVIVGLIIGSMIIALYLP
                     IFTAITQSGY"
     gene            complement(6686..7783)
                     /locus_tag="PK733_11910"
     CDS             complement(6686..7783)
                     /locus_tag="PK733_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ11282.1"
                     /translation="MTENKTGLEYPVSMKGLLTLSSGKGASDLHLVCGSPPQIRIVGN
                     IEALDYPVIRPYQMKKLIFSVLEDWQQDKLKKKWELDFSYSIEGVARFRGNIMRQRGS
                     YAAAFRVVPYIVPQFDSLGLIPEIKNLCNLARGLVLVTGATGSGKSTTLAALTDIINK
                     TRCVSIITIEDPIEFLHKHNKSTVRQREIGTDTHSFADGLKYALRHDPDVILVGEMRD
                     EESMAIALTAAETGHLVFSTLHTQTAPQAISRIVDAFSNEKKNQIRQQLAGTLKAIIS
                     QQLIPSITGEGRVLALEYMVSIPAIRNLIREGKDHQLYNSIQTGQNFGMRTMDQSLAR
                     LYKQGKISYESVYEHCVEKQEVDRLINMMYI"
     gene            complement(7804..9789)
                     /locus_tag="PK733_11915"
     CDS             complement(7804..9789)
                     /locus_tag="PK733_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ11283.1"
                     /translation="MTIKFKNYRVGKKRTDDSGCLKNGNVVSLILIMFLCFNFAFSIN
                     SDILQKVYADAPSEVNVPNSSDEGHIIYIVEKGDTITEIAREYGVSKANIIKINDLQT
                     PSIIIPGQELKIPKKDTGNVNSIINNKDANLGSNTTETINNTAADLKASTNNTVSTGI
                     VPKAVSITFKNADLRDILSTLALAMNVNIILAEEPVTASFQIKNTAPRTALDYLLKTL
                     GLEYVESGNLIVVGNSETLQRDFMNQMTLTRFNLKYITSDIISSQIDALGISVKKITL
                     ENNEKAIWVQGTPPSLSKVRELISMIDLKENSPDENKTENGINLSSFKLEYVTAQTLD
                     KLIHQMGINSKTLIVDANPRTIWVDAAGQALKDIEQLIQKVDVVDNYAIESTMPLELI
                     PYKLSFVSPEDLNEIIQETGIDVKTIYFKSNPNKIWVDSRSKDILDFEELIVKVDLME
                     NTTWPLNIVTQNLKNITASKFKTIANEIGITTQIITLDSNAHTVWLVGDYRELLDIKS
                     LLKELDNNIVSDGANFFTYHLNYISPSEAVKRFGYLNVEDINVIALEYPFFSKEILVV
                     GPADRKPVITEMLNNIDVSGEKIRVPIDYSNSASGQSRLTSRRDLLVTLTKIPASSFF
                     ISNNVSRDDTPLYIMWVEETPENISKIRNMIDTISNP"
     gene            complement(9891..10433)
                     /locus_tag="PK733_11920"
     CDS             complement(9891..10433)
                     /locus_tag="PK733_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11284.1"
                     /translation="MNDLNKFKEAISDFFKKNKSLVYLVPLLMVLLIIVIVLYSCGDK
                     AATVNANKTQNPTTNQTTNNKSNATGNDKVEVSGNKVEVLPQTERTADKENQNVKTNS
                     NENKNTSNPVNPFDLPMRLSGVLTGSNDGSIAIIEISQNSYIVREGEVINNTWKVSKI
                     EENGVLLVNGEKESYITFSD"
     gene            complement(10711..11754)
                     /gene="ltrA"
                     /locus_tag="PK733_11925"
     CDS             complement(10711..11754)
                     /gene="ltrA"
                     /locus_tag="PK733_11925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOJ11285.1"
                     /translation="MQYEVTVPTVKDRVVQQAIVNIIEGIFDKDFHPSSYGYRPGKSQ
                     HKAVAKAEKFLNTYNLTYVADMDLSKCFDTLDHSIIMREVSAKISDSKVLKLIEKFLK
                     AGVMKDDSFSETELGSPQGGVISPLLSNIYLNVFDQKMKDKGIRIVRYADDILIFARN
                     KADAGNHLAYARRILEEELKLTVNEEKTTLTNVSKGVAFLGFIIFPHYVTIHPKRLKR
                     FKDKIRRITKRNCGIPIEDVIKFLNPVLRGWMNYYRAANIKSLCKDIMAWIRRRLRML
                     RMKQWKTYKKMHKDMRRKGIAGNGEKMDVRRWKNSKVHIIHILLPNKYFEELGLIDIT
                     TYDVGVLSSFYAG"
     gene            complement(11792..>12149)
                     /locus_tag="PK733_11930"
     CDS             complement(11792..>12149)
                     /locus_tag="PK733_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11286.1"
                     /translation="IRKLSATLTDSQIVDHLNESGLHTPEGRKFTVGSIKWIRYKNKI
                     PVLSMHSKGLSVKEVAARFNVGMDTVYYWISRGVLEAKKVAPGWPWDIQIDENKEAEL
                     REWVQKSGHLTKTKAR"
BASE COUNT         3505 a         2452 c         1771 g         4421 t
ORIGIN      
        1 ttaacattaa tatcgccgtt aatggtagga tttccagata aagtagcatt cccggcaaca
       61 ttgacattac catttatagt accattgttc attaccaagt tttcatttac attcacatca
      121 ccatacattt tagcgccacc atcgaaagtt aaagtgccat ttatgaaaat tatattagat
      181 tcatctacta accctaaagt atgcccttgg cttacagcaa gatctccgtt tgtatataga
      241 tgcttaactt taattttggc tcctccattt attcgtccgc taaaatcatc agttacaaat
      301 atagttcctt cttcaaatac acactcatta ccggcaaatg ttaattcgct cccataaata
      361 aaaacacttt catttaaaac aaactgacgg aatttttcct ctggagttag ttctaccggt
      421 agtgacaatg ctacttgcgt tcctaaactt gtaattttaa actcgttaaa cttattattt
      481 gcaatatcag ttataagctt tgctgaaact aaagtactat tgtccagcga aaacgaaaca
      541 tcctttattt ctttaaactc tgtttctgta gctgcttgcc ttttaagata attattacct
      601 tcagcatctg cagcaatata gtaaataatg ctttgatcat ctttatcggt aattatcagt
      661 tcattaggtt tagcaatatc aaaatttata gctttagaaa atttaatatc ttcagttaaa
      721 taagcagcag ccgctctgat ttcatgtctt tgtgcagatt ccttcaactg gttatttgcc
      781 atagcattat tcatagcaaa gacttgaatt ataattaagg ttaaaaaact aattattgcc
      841 agggtaataa ccaactcaac tagtgttaac gctttttgat tacttcgtat attttctctg
      901 tcgatattat cactaccttt ttttgtaaac aatagattgg taaaaaaact tccttgaaac
      961 atttattaca cctcccttaa acgcactgac atttttatag gatttacata tgaatttaaa
     1021 ggttttactt ctatgcttat ttgtattaat ttgtcaattc ccttatcttt ctcaatatat
     1081 aatttaatgt cataacttat acctaggcta tcactagcaa ccttgcaaaa agcattattt
     1141 atcgtctcaa tatagccttc gttaagtaaa cgtgctttga gcccggcaaa atttttccag
     1201 tcgtactggt atgctttaat ttcatcaact atacttgtag aatatgctag catctgtaat
     1261 ctttctctac tggtatttct cgctttatat ccggtaaata gcaaattaag aaaaattcca
     1321 gttatgacta ccataatagc cacagcaatt aatacttcaa caatcgaaaa acccttctta
     1381 tttataaatt tgaacatcct gcattaacct cattctacta aaaacttcaa taaaaaacta
     1441 gataaacttt tgattttttt aattcttaca ctataaccct taatacttcc tccacggtag
     1501 ttattccttt ttttgctttt tggagaccat cctgtttcat tatcagcata ccttctctta
     1561 ttgctgcttg acgtatttcc tttgctgatg cccttgaaag cattaatttc tgaatacttt
     1621 ccgtcacttt caaaaactca taaataccta ctctgcctct ataacccgtc ccattgcaat
     1681 acacacatcc ctttgatttg aaaagctgta tttcttcttc gtctccttca aatggaaaat
     1741 caggtattga atttattaat tgttgccttg tcacgatata tggttgttta cattctttgc
     1801 aaagaaccct catcaatctt tgcgctacaa cacctattaa agaagaagct gtaaggtatg
     1861 gttcaatacc catatcacta agtcttgtaa ctgctcctgc cgagtcgttg gtgtgcatgg
     1921 ttgataaaac aagatgccct gttaaggcag attccactgc tatcctggca gtttcatggt
     1981 ctcttatctc tccaaccatt attatatccg ggtcatttct taatatggca cgaagtcctg
     2041 atgcaaaagt cattcctgct ttcatattcg cctgaacctg ggttaagcca tccattctcc
     2101 tttcaatagg atcttccaga gttataatat ttctgtccgg ttgatttaat ttggcaagtg
     2161 aagcatatag ggttgtgctt tttccactgc ctgttggtcc tgttatcaat ataaagccat
     2221 atggagattt tgtggttatc tcatattttt ccaattcgta ctcgttgaat cccagttctt
     2281 tcaatgtaat taacctatca ctccggttta acaacctcat ggtaattttc tcaccatatg
     2341 ccgatggcat tgatgcaaca cgtacgtcaa ttatattgcc atcaactttt aaggtaatcc
     2401 ttccatcctg aggtatgcgt ctttctgcta tatccatatt agacataact tttaaccttg
     2461 atacaagagt tggatgtaaa ttaatttgct gctgcattat ttcatgtaaa acaccatcaa
     2521 ttctgtatct gattctaaga cgcttctcct ggggttcaat atgaatatca ctcgctcctg
     2581 cttttactgc ctgattaata atttggtttg caagctgaac tgcaggtttt tcatttaaat
     2641 catcattttc agtttcatct tcctctattt cttctatttc atctgtttcc tgcaaagcca
     2701 tactcatgtt tataaaccgt tttattgcag tttcaagttc tttatcatta ataacaactg
     2761 gttttatgct gcagcctgta ataatcctta agtcatcaat agcaattatg tctccagggt
     2821 ttttcattgc cagtaaaagc ctatcatttt cgaaaccaat gggcaataca ttatatcttt
     2881 tcgccaattc aggtgttatc aggttagcag cagacatatc tatttcataa ttattcattt
     2941 caaggaactg aactcccgcc tttcttgcca atatccttga aatatcattt tcagtgcaat
     3001 aacctaattc aacaagagtg ttgccgatta aacctttttt cttattttgt tcttttgttt
     3061 tctgcttttc taatgcagac tccagttgct cctttgtgat tataccttca ttaattaaca
     3121 attcaccaag aaggctattt ttagctgtca atggcaacca tttcacacct ttcctttttt
     3181 actttcttat attataggcg actatgttaa tatcagcaat aagtttttga gaatcatcat
     3241 ttctttcaat atttacttct cgtataacaa aaattctttc attatctttt ataccaacaa
     3301 taaacttaac taaatcaaaa tactctccgg aagcgttaac atgaaatgga agtttagcca
     3361 gggtttcctc ctgtgtacct tcatcaaacc tgatttcacc aagctcaatt gaattttcgg
     3421 aagccaactt gctcaatccg agaattatat taccttcttc aggagtttca ggaagagctc
     3481 ttctatattt atcaagcata cccagcattt cgtcggctct ttgctcataa cctttcaaat
     3541 agatcaattt ttcatttagc ccgtcaattt cattattaac catggatata ttatcaagat
     3601 taccttttaa aataatgatt tccctaatta agaggaatga agctattaaa atacaaatac
     3661 ataaaactat gacaggtata cttaatttcc gtttatacat tttccaatct cactccattc
     3721 ctttaactgt tttgattatg ctcatatcaa tttcagaatt tatctcatac tcataaactt
     3781 ttttttcacc atcatcataa tcctgtaagt acttgcatgc tatgttcttc aatatttcct
     3841 ttttctctat atcatttagc cattgagcca taattacttt atttgtcgat cttcccgcga
     3901 tgacacatgt tcctttttta ccgtcatagt caaggcttat actatcaaac cacattccct
     3961 gaggtattgt ttcagcaaat gtatttaaaa gagtgtccca attgtaactg tcaatatttg
     4021 ccttttctat aagtttttct tgcttcttta ctgaattata aacatctgaa tattttccat
     4081 atgtgctaat cattgattca agctggtttt tttcaatatt taaactgttt aattgattaa
     4141 cataaataat gtttatcaat ataagcataa tataactcat tattaaagca gaaacaacaa
     4201 taacgcctat catggaaaat agcctttttt tcttgtttct tatgatgaga ttcttatatt
     4261 ctaatggcat caaactaatt ttgctcaaaa tatcaatcct ccaatccatg tagggcaaga
     4321 cttaaagcaa tagcaaattt actaggttcg taatcgtcaa ctataacttt atctttattc
     4381 tttttagata gcacactaaa caaattttca tatggttgca aaatttctac attcataccc
     4441 aaataatcag caagtccttc gtagacagcc tcattactgc atgcacatcc tgtcagaaat
     4501 attttttcaa taaaagaatc cctgttttgt gattgaaaat atgatataga ggaccgtatt
     4561 tcatctccta caaaacttac gatatttaga atcaacttat tttgattgca ctgcaggttg
     4621 ctatgttcaa aactatgcag accctctttc tccataacag cagtattact tatgccgact
     4681 agctttgtat tttctgtcgg aactgtatct ctatatgaat aacctgcatt tgcattccag
     4741 gaagcgctaa atttctcttt gttaataata tctatttccc tgtgcatgtt ctcttttagt
     4801 aatatagttc ttgcaaatgc cggaagatta ttatttacta ttaataaatt acatgcttca
     4861 gaagaaagat ttactactac tgaaacacta ttgcggtatt ccccgggtac aagtttatcc
     4921 ataaccaaca cggaactctt tatatcttta atactggaat taactgtact aaaagcatta
     4981 ataaactcat aaagcatttt tttaccagct gccactaaca gcacattgta atatgagccc
     5041 gattcatttg cattctcact gataattgta taatcaagtt catagtcatc aatattaaca
     5101 ggcatatagt ctgcaacttg aaattttatc aaatttgaaa gtttatcaag gggcatttta
     5161 ggtaatgtga tgaatctcat tattatgtca ttattactaa tcccaagtat tatttctttc
     5221 gattttattc tattcttttt ccacaattcc tcaagtacat tcttaagttt atcctggtta
     5281 acaactttgc cttcttttac tacatcttta tcaagaaaaa cctttcctgt tttttgtatt
     5341 acaggtgttt gagagcttct gctcatttca acaaaatgta tttgggaaca actaatatca
     5401 attccgatta cacttttttt atataaactc attttttagc cctccataga attaatatcc
     5461 tgactgggta attgctgtaa aaatcggcag ataaagcgct ataatcattg aacctattat
     5521 taaacccacg attaccagca ttattggctc aattaatgct gttattcctt ttgtcagagt
     5581 cgtaacttca tcttcataaa attccgccat tttatccagc atgctggaaa gtgaaccggt
     5641 ttcttctccc acagcgatca tttgaatcat cataggagga aacaagtagc tttctttaag
     5701 ctgatcagat atatttttac cttcttctat tttcttcaca gtattattta tgacttcatt
     5761 taccagtaag cttcccgatg taggtcccgc tccctcaaga gcttgtataa taggtattcc
     5821 accttcaagc aatgttgcaa gagttcttga aaatcttgcg agcacagctt tatgcattat
     5881 tggaccaaaa gccggtatct tgaattttat actttcccaa attcttttac ctgccggact
     5941 tcttacaaag atgtaaataa caaaaacaaa caccgctaac acagcaatcc atataaacca
     6001 actccctctt attgaatcgg aaagtgaaaa tatcatagcg gttatagctg gtattgcagc
     6061 attttccggt ataaagccct taaaaatcgg caccataaaa accaacattg ctacaaaaag
     6121 tattaatgca aatcccgcta ccattctggg ataaaaagtt gatgctttaa tattgtcaat
     6181 aagcaccttt tctttctgca gctgatctgc cagtctggtt aaagaatcat caagaatgcc
     6241 tccaacttca ccggaatgca acattgatat atataaatcc gaaaatatct tcggatacat
     6301 actaaaagca tcagttaaac ttctgccgcc ttcaacattt tgagctattg ttataagtgc
     6361 agcttttagt gtcggatttg ttgcctgctg gcctatagta aacaaagctc ttgtaacagg
     6421 tatgccggca tttagcatag ctgccatttg tttgctgaac attgccagtt caccaattga
     6481 tacttttttt tgaaataacc cgctagtttt tctgactgtc gtatccattt gctttaaatc
     6541 aacaatcatc agttccatct ttttaagttt atctatagct atcgagttat tatcagctga
     6601 tatcttacct ttaacaattt ccccctgcct gtttatacat tcataaatat aatctgccat
     6661 tttaatttcc tcttttacct ctttttcaga tatacatcat gtttataagt ctgtctactt
     6721 cctgtttttc aacacaatgt tcatatacac tttcataact tatttttcct tgtttataca
     6781 atctggcaag agattgatcc atagtacgca taccaaaatt ctgtcctgtt tgaatagaat
     6841 tgtatagctg atggtccttg ccttccctta ttaaatttct tattgcaggt atactgacca
     6901 tatattccag ggcaagaact cttccttcac ctgttattga aggtattaat tgttgagata
     6961 ttatagcttt caaagtacct gctaattgct gtcttatctg gttctttttt tcattgctaa
     7021 aagcatctac aattcttgat attgcctgag gtgctgtttg ggtatgaagg gttgaaaaaa
     7081 ccaaatgtcc tgtttctgca gcagtaagtg caatcgccat gctttcttca tccctcatct
     7141 ctccaacgag aattacgtca ggatcatgcc ttaatgcgta tttcagtcca tctgcaaaag
     7201 aatgtgtgtc tgttcctatc tctctttgcc ttacagtaga tttattatgc ttatgcaaaa
     7261 attctatagg atcttcgatt gtaataatac ttacgcatct tgttttattt atgatatctg
     7321 taagcgctgc aagtgttgta gattttccgc tgcctgtagc acctgtaact aatacaagac
     7381 ctcttgctaa attgcacaga tttttaattt ccggtataag accaaggctg tcgaattggg
     7441 gtacaatata aggaacaact ctgaaagctg ctgcataaga tcctctctga cgcattatat
     7501 tgcctctgaa acgggctaca ccttcaatag aataagaaaa atccagttcc cacttttttt
     7561 tcaatttatc ctgctgccag tcttcaagta cagaaaaaat caactttttc atttggtatg
     7621 gcctaataac cggataatca agtgcttcta tatttccgac tatccttatt tggggaggag
     7681 aaccacagac taaatggaga tccgaagctc ctttcccgga agataaggta agcagccctt
     7741 tcatactgac cggatattcc aatcctgttt tgttttctgt catcctattc cgacctttct
     7801 cccctaagga ttacttatgg tatcaatcat gtttctgatt tttgaaatat tttccggggt
     7861 ttcttcaacc cacataatgt ataagggagt atcatccctg gaaacattat tagaaataaa
     7921 aaagcttgaa gcaggtattt tagtcagtgt tacaagaaga tcccttcttg aagtcaaacg
     7981 ggactggccc gaagcactat tggaataatc aatgggaaca cgtatctttt cacctgaaac
     8041 atcaatatta ttaagcattt ctgttattac cggttttctg tctgccggac ctactacaag
     8101 tatttctttt gaaaagaatg gatactcaag tgctatgaca tttatatctt caacattaag
     8161 atatccgaat cttttaactg cctctgaggg gctgatgtaa ttcagatgat atgtgaaaaa
     8221 gtttgctcca tcactaacta tattgttgtc aagttctttc aaaagagatt taatatcgag
     8281 gagctctcta taatccccaa ctaaccatac agtgtgcgca ttagaatcca aagttataat
     8341 ctgcgtagtt attccaatct catttgctat tgttttaaac tttgaagcag taatattctt
     8401 tagattctgt gtaactatat ttaaaggcca agtagtattc tccatcaaat caactttaac
     8461 aattaattct tcaaaatcca gaatatcttt gcttcttgaa tcaacccata ttttgttggg
     8521 atttgatttg aaatatatag tttttacatc tatcccggtt tcctgtatta tttcattcag
     8581 atcctcgggg gatacaaaac tgagcttgta tggtatcaat tccaaaggca tggtactctc
     8641 gattgcataa ttatccacaa catcaacctt ttgaataagc tgttcaatat ctttcaaagc
     8701 ctgcccggct gcatcaaccc atattgtccg gggatttgca tcaacaatca gtgtttttga
     8761 attaatgccc atctgatgaa tgagtttatc cagggtttga gcagtaacat actcaagttt
     8821 gaaagatgat agatttatac cattttcagt tttattctca tccggactgt tctcttttaa
     8881 atcgatcata gaaataagtt ccctgacctt tgaaagcgac ggaggagtac cctgcaccca
     8941 aattgctttt tcattattct ctagagtaat tttcttaaca gatattccta gtgcatctat
     9001 ctgactgctt ataatatccg atgtaatata tttgagattg aagcgggtaa gcgtcatttg
     9061 attcataaaa tctctctgaa gagtttcgct attgcctaca acaataaggt ttccactttc
     9121 tacatactcc aagccgaggg tttttaatag gtagtctaaa gctgtacgcg gtgctgtatt
     9181 ttttatctgg aaagaagctg taacaggctc ttcagctaaa attatattaa cattcatggc
     9241 taatgcaagt gtagaaagga tatctcttaa atccgcattt ttaaaggtaa ttgacactgc
     9301 cttgggaaca atgcctgtac tgactgtatt atttgtgctt gcttttaaat cagcagcggt
     9361 attgttgata gtttcggttg tgtttgatcc taagttggca tctttattat tgatgataga
     9421 attaacattg cccgtatctt tctttggaat cttaagctcc tgaccgggaa ttattatact
     9481 tggtgtttgc aaatcattaa tttttattat atttgcttta gatactccat attctctagc
     9541 aatttcagta atagtatcac ctttttcaac aatatatata atatgtcctt catcagatga
     9601 atttggtaca ttaacttcag agggtgcatc agcatatact ttttgaagta tatctgagtt
     9661 tattgaaaat gcaaaattga agcacaaaaa cataatcaat attaatgaaa caacattacc
     9721 attcttcaag caaccgctat catctgttct ttttttacca accctgtaat ttttaaattt
     9781 aatagtcata aatcaccatt tgccccctca actaaattgc tagtaacatg ttaatcaacg
     9841 taaaatcatt tttgcgttat atatactaat gtcgtaaata agctttaagt ctagtctgaa
     9901 aaagttatat atgattcctt ttcaccattt acaagtaata ctccattttc ttctattttt
     9961 gaaactttcc aggtattgtt tataacctct ccttctctta caatataaga gttttgagaa
    10021 atttctataa tagcaataga accatcattg cttcctgtaa gaacgcccga aagcctcatg
    10081 ggtaaatcga aaggattaac ggggttactg gtatttttat tttcatttga attagtcttt
    10141 acattttggt tttctttatc tgcagttctt tctgtttgcg gaagcacttc tactttattg
    10201 ccgctaactt caactttatc atttcctgtt gcatttgatt tattattggt tgtctgattt
    10261 gtcgttggat tctgcgtttt atttgcatta accgttgcag ccttatctcc acaagaatac
    10321 aatactatta ctataattag tagaaccatt aataatggta caagataaac aaggctttta
    10381 ttttttttaa aaaagtctga tattgcttct ttaaatttat ttaaatcatt cataaagatg
    10441 cctttcccat taaaatattt cttgtatgaa tccgtagtta tttagtagta atataatgtt
    10501 tatattagtt accataatat cattatacga cacttttgta ttaaatacca tatgtaaaag
    10561 caacattatg tcgaagaata ttatggtttt tttgtaaatg ttacgtatta attgtaaatc
    10621 gagtagctag aaaacaggag taatttcctg ttttctttgc tctcacagaa ccgtacatac
    10681 gtttcccgta tacggctcct gaaattggta tcatccagcg taaaaggatg acagtacccc
    10741 aacatcataa gtcgtaatat ctatcagacc taactcttca aaatatttat taggtaggag
    10801 tatatggata atatgcacct tggagttctt ccagcggcgc acatccattt tctccccatt
    10861 tccagcgata cctttccgac gcatgtcttt atgcattttc ttgtaggttt tccactgttt
    10921 catccgaagc atcctaagtc ttcttctaat ccaagccatg atatctttgc aaaggctctt
    10981 gatattagct gctctgtagt agttcatcca gcccctgagg acaggattca agaattttat
    11041 gacatcctct attgggatgc cgcaatttct cttggttatt ctcctgattt tgtccttgaa
    11101 tctctttagt cgttttgggt gtatggttac atagtgcgga aatattataa accctagaaa
    11161 cgccacgccc tttgagacat tcgttagagt tgtcttctct tcattgacag tcagctttaa
    11221 ttcttcttcc agtatccttc gggcataagc taggtggttc cctgcatctg ctttatttct
    11281 agcgaagatt aggatatcat ctgcgtacct cacgatacgg atgcccttat ccttcatttt
    11341 ctggtcaaag acattcaagt agatattgct gagcaatggt gagatgacgc caccttgagg
    11401 acttccgagt tctgtttcac tgaatgaatc atccttcatt actcccgcct taagaaactt
    11461 ttctattagc ttcagtactt tgctatcgct tatctttgcc gacacttccc tcatgatgat
    11521 actatggtcc agtgtgtcaa agcatttgct aaggtccata tccgcaacat aggtcaggtt
    11581 gtaggtgtta aggaatttct ccgcctttgc aactgccttg tgctgtgatt tgcctggtct
    11641 atagccatag ctggacgggt gaaagtcttt atcaaagatt ccttcaatga tgtttactat
    11701 tgcctgctgt accaccctgt cttttacagt tggcacggtg acttcatact gcacacctcc
    11761 caagactgtt ttggaatttt aatatttatc attaacgggc tttagttttc gtaagatgcc
    11821 cggatttctg tacccattcc cgcaattctg cttctttgtt ctcgtctatt tgaatatccc
    11881 aaggccatcc aggagcaact ttctttgctt ctaaaacacc tctgctaatc cagtaataga
    11941 cagtatccat tcccacgttg aaacgtgctg ccacttcctt tacagacaag cctttgctat
    12001 gcatggatag cactggtatt ttgtttttat atcggatcca cttaatggaa cccacggtaa
    12061 atttcctacc ttcaggtgta tgtaatccag attcatttag gtggtctaca atctgactat
    12121 cagtcaaggt agcagatagt ttgcgaatc
//
LOCUS       DAOSDD010000132        12125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2033_length_12125_cov_4.849130, whole genome shotgun sequence.
ACCESSION   DAOSDD010000132 DAOSDD010000000
VERSION     DAOSDD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_12125_cov_4.849130"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2402
                     /locus_tag="PK733_11935"
     CDS             <1..2402
                     /locus_tag="PK733_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11287.1"
                     /translation="TELVDVIEGMVSGYSATTLGSQTVTMSYGGKSVSVDVTVRDYVT
                     SINITAEPTKKLYYIGDALDLTGMVVESMYASGAKAILTEEDYSVSGFDNSKEEIEQL
                     ITISYIDGDGTYTASLNIRVTADTIDSIIVSVKPDKLIYYITEDTALDLTGGKITVKW
                     AGAGDVPVDLTDSLILVSGFDMDTAGTKSITLSYGGKSTSFTIEVKYRTYKVEFSTSG
                     GSTIPEQVVNKNGTVTLPVAPPTKGGYTFEHWYLDDPAIDFDFNTPIVADIILNAKWA
                     PVIASVASVSAVNGKTTVILAAQPAVAPALSDFTLMVYKDGDTTGSAITPASLVYDGN
                     KTVSINFAAIAQTSSTQSIVIGVSLSGGVELKTAPYTISATSSGGEVPGGGDIPGGGG
                     TPGGGDIPGGGGTPGTGGTPGGTGGDDKKPDEKPEDKPEDGKEDEKPAIGSKTVNLPD
                     GTTANIITKDDFKELISDIGQGGTIEIDMSRIGKSNSIILSLPSIEPEGKKDSTLIVT
                     TSTGQVTIDIDKLLQSGLGNKTDAEIIISPETGTAEIFVGGKKLDLPAFDNPISISMG
                     YAVKPVQNTDYLVAYWEDENGNRTILPMSYFENGAMNFSPPSSGRVGVMHNEVMFEDT
                     LDRWMDDDVCYLAARGIIKGTGGKNFSPDANINRADFVVLLVRMLGVSADTIENFDDV
                     DSNAYYARELAIARALGIVNGIGGNKFNPNGEISRQDAFVMLYRAMKKYSRLPDSISN
                     SNPEFKDFGDVSDYALESIESFTKSGIVKGSGGYLNPKDTTTRGEAAALFRRIIKYFM
                     K"
     misc_binding    2627..2965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3154..5202
                     /locus_tag="PK733_11940"
                     /pseudo
     CDS             3154..5202
                     /locus_tag="PK733_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559099.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            5424..5786
                     /locus_tag="PK733_11945"
     CDS             5424..5786
                     /locus_tag="PK733_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ11288.1"
                     /translation="MAIFENIGKKVTEAAQVAAKKSGELVEVTKLNMNISSEEDRIQK
                     LYEQIGREIYKYFTSEKDIPEELKDVCAEIRSREETIKGIKARILEIKNLQECNACGA
                     ELERNIAFCPKCGAKQEH"
     gene            6140..7204
                     /locus_tag="PK733_11950"
     CDS             6140..7204
                     /locus_tag="PK733_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ11289.1"
                     /translation="MSVTVKDVARMAGVSTATVSRVINDDPRISRETSEKVKKCISDL
                     DYRVNNIARSLKTSRTYTIGFIAPEITNDFFMSIAKGVEDESRKYGYSIIVCNSNESI
                     KEEEDRIRLLSEKCVDGIIIIPASDEGKHFNHLKELNMPVVLVDRLVNNFESDAVLVD
                     NINGTYSAIEYLINMGHGRIGFISGDLKLTSAMERYQGYKRALSDYCIPYDEKIVKFG
                     DFHIRGGYDMMKKLCEADNPPSAVFISNYFMHVGATKYLMEEDINKGKRSSGQNNKNI
                     IIASFDDMELSTLLGFCKLRVAQPMMEIGSKAAQLLLGRINNDFERINNDNDAAAGDS
                     QNNRVIRLKTRLVVNQQIIS"
     gene            7369..9189
                     /locus_tag="PK733_11955"
     CDS             7369..9189
                     /locus_tag="PK733_11955"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOJ11290.1"
                     /translation="MANPLSINVNPPRQRLRGSLPKVGIRPAIDGRRRGVRESLEEQT
                     MNMAKAAAKLITENLKHPNGMPVECVIADTCIGGVAESAMAAEKFAREGAGVSLTVTP
                     CWCYGSETMDMDPFTPKAIWGFNGTERPGAVYLAAALAAHSQKGLPAFSIYGRDVQDA
                     NDTSIPQDVRRKILQFAKAGLAVAEMKGKSYLSLGTVAMGIAGSIVNEEFFQNYLGMR
                     NEYVDMVEFIRRVDEEIYDKDEFEKAMKWVKENCKEGCDNNPEAVQHSRERKEKDWEM
                     SVKMAIIARDLMIGNPELADMGYGEEALGHNALAAGFQGQRQWTDHFPNGDFMEAILN
                     SSFDWNGIRQPFIFATENDSLNGVAMLFGHLLTNTAQVFADVRTYWSPEALKRVTGMK
                     PEGLAANGLIHLINSGAAALDGTGEQTINGKPAMKPFWEITPEEAAKCLDATQWRPAS
                     LGYFRGGGYSSDFLTKGGMPVTMSRVNIIKGLGPVLQIAEGYSVELPEKIHEILDNRT
                     DPTWPTTWFAPILTGRGPFEDVYSVMANWGANHGAFSYGHIGADLITLASMLRIPVSM
                     HNVSEGRIFRPSTWSAFGTCELEGADYRACANFGPLYGRK"
     gene            9683..10390
                     /gene="sigI"
                     /locus_tag="PK733_11960"
     CDS             9683..10390
                     /gene="sigI"
                     /locus_tag="PK733_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HOJ11291.1"
                     /translation="MKYTSINERVQDIKGNTEEISRFIEEYKPFIASSAGKISGKYLR
                     YGDDDELSIALMAFTESINSYDISKGKFLTFAQGVIRRRLIDYYRKERKHINVISLNE
                     YYYENTDDMDLSTEESIGKFQLDEINEYRKLEIAELDKELKGWDISFSDLVKVSPKHK
                     DTRETCKVIIKHILSKPDLLEFIKNKKYIPVSDLGKSLNLSRKKIDRLRKYIIAMLII
                     ISGDYQYLKEYIRDYIL"
     gene            10456..11748
                     /locus_tag="PK733_11965"
     CDS             10456..11748
                     /locus_tag="PK733_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HOJ11292.1"
                     /translation="MKGIVVETKGRFAVVMKNDGEFVKIRNHGNLIVGYETDISERNT
                     EFVNSATTRHAGIIYRMASVAAALILIFGMSYVVYSYNVPYAYISVDINPSIEITTNI
                     FDRIIDIKGVNTDGEKLIALEDYVNKKIDKGIGHIINSAVNEGYIKPENGNMVFMTVS
                     AKNEKKAGKLEKNIEAVALEEIQASGADSSLYIEEVAVDKHDEAEILGISPGKLVLID
                     KLRESDSQIKPSDYADVSVKEIMKSVIENKKEEIFQNKMLKNEEKAKKREEHELEKEE
                     NKQQGSIQQGNKLKEGKQQDKQNNPVLNAKMKNNNNEKPKNEPKNEPKNEAKNEAKDG
                     AKNPVKGNPNRKNEDIKEDNSEDINRHSDDDDINSIEDDEKDTDYSGFNKEKVNKGNI
                     KPNEKVNEKPNEKVNEKVNEKVKGKGNKKSNEKGNVKK"
BASE COUNT         4244 a         1860 c         2673 g         3348 t
ORIGIN      
        1 agacagagct cgtagatgta atagaaggaa tggtaagcgg ctatagtgca actaccttag
       61 gtagtcagac agtgacaatg agctatggcg gcaagagtgt atcagttgat gtaacagtga
      121 gagactacgt aaccagcata aacataacag cagagcctac taagaagcta tattatatag
      181 gagatgcctt agatttaacc ggaatggtag tagaaagcat gtatgccagt ggagcaaagg
      241 caatcttaac agaagaggac tacagcgtaa gcggcttcga caattcaaag gaagaaattg
      301 aacagctaat tacaataagc tatatagatg gagatggtac ttatacagca tcgctcaata
      361 taagagttac agctgatacc attgatagta ttattgtaag tgttaaaccc gataagttga
      421 tatactacat tactgaagat acagcacttg acctgacagg cggcaagata acggttaagt
      481 gggcaggagc aggtgatgta ccagtagact tgacagattc tctcatactt gtttcaggat
      541 ttgacatgga tactgccggc actaaatcca taacgctaag ctatggtgga aagagcacca
      601 gctttaccat cgaagttaaa tataggacat ataaagtaga attcagtaca agcggcggaa
      661 gcacaatacc ggaacaggtt gtgaataaaa acggtacagt aacattacca gtagctccac
      721 ctacaaaggg aggctataca tttgaacatt ggtacctgga tgatccggca atagactttg
      781 attttaatac tccaatcgta gcagatatca tcttaaatgc aaagtgggct ccggttattg
      841 caagtgttgc aagcgtttcg gctgtaaatg gcaagaccac agtcattctg gctgcacagc
      901 cggcagtggc gccagccctg agcgacttta ctttaatggt ttataaagac ggcgatacga
      961 ctggctcagc tattactcca gcaagccttg tatatgatgg gaataaaact gtttcaataa
     1021 acttcgcggc tatagcacaa acttcatcaa cacagagtat agttataggg gtatctctgt
     1081 caggaggggt agaattaaag acagccccat atacaatatc tgcaacaagc agcgggggcg
     1141 aggtgccagg tggcggtgac atcccgggtg gcggaggaac accaggtggt ggcgatatcc
     1201 cgggcggcgg gggaacccca ggcaccggtg gaactccagg tggaacaggc ggtgacgata
     1261 agaagcctga tgaaaaaccc gaagataagc ctgaagatgg gaaagaagat gaaaaacctg
     1321 ctataggaag caagactgtg aatcttcctg atggaacaac tgctaatatt attacaaaag
     1381 atgatttcaa agaacttata tctgatatag gtcagggtgg tactattgaa atagacatga
     1441 gccggattgg taagagcaac tcaattatac tctctcttcc gagcattgag ccagaaggca
     1501 agaaggattc aacattaata gttacaacat ccaccggtca ggtaactata gatatagata
     1561 agctgctcca aagtggatta ggtaacaaga cagatgcaga aattataata tcgcctgaaa
     1621 ccggaacagc cgaaatattt gttggtggta agaagcttga tttaccggct tttgataacc
     1681 caatcagtat ttctatgggc tatgcagtga agcctgttca aaacaccgat tacctggttg
     1741 catactggga agatgaaaat ggtaatagaa caatacttcc tatgagttac tttgaaaatg
     1801 gtgcaatgaa cttttcacca ccttcttcag gaagggttgg cgtaatgcac aatgaagtga
     1861 tgtttgagga caccttggat cgctggatgg atgatgatgt ttgctatctg gcagcaagag
     1921 gaataattaa aggcacagga ggcaaaaact tctctcctga tgcaaatatt aaccgtgctg
     1981 actttgttgt attgcttgta cgtatgctag gagttagtgc agacacgatc gaaaactttg
     2041 acgatgtgga tagcaatgcc tactacgcca gagagcttgc aatagccagg gctttgggta
     2101 tagtaaatgg aataggcgga aacaagttca atccaaacgg cgaaataagc cgacaggatg
     2161 cctttgtaat gctctacaga gctatgaaaa agtattcgag attacctgac tcgattagta
     2221 acagtaatcc tgaatttaag gactttggtg atgtctctga ttatgccctt gagagcattg
     2281 aaagctttac aaaatccggt atagtaaagg gctcaggagg ttatttgaat cctaaagata
     2341 caacaaccag aggagaagca gcagccctat tcaggagaat tataaagtat ttcatgaagt
     2401 gaagatgggt tttttagggg aaacaaagga aaaactaatg aaaaaatgag gatatgaagg
     2461 gggcagagat tctgccccct ttgctttcaa aaaaaatagt tttcttcact tttatcttta
     2521 agaatacttg ctaccgggaa atatgcacca cataattttt ttaatacttt acattatatc
     2581 cgtaagtatt tataataata tatataaata tgaatgttag atacagataa atgtaatgaa
     2641 aagggataat aggctaaatt tcttcatcta cagagaaccc tggttgctgt gaatgggcgt
     2701 tgcggaatta gctgaacagg ccttggaact ttgcaccgaa actatatttg agtattaggc
     2761 tgcaacgggt gtacccgtta ttgtactaaa gaattatgca gactgttaaa cgtactgata
     2821 tgcaaactgt tataaggatt attatgcaaa ctgtatatta ttgaagaatc tgtacagctt
     2881 tccgcatttg ttcttgatga gacctttgtt gtgagacaga ggtaaattgg agtggtacca
     2941 cgaagcatgt gctctcgtct ccaggatata tattcctgga ggtgggagct tttttgatat
     3001 agtttaaaac tagagcttaa taacaacttt ttagtgattt tatatttaac gacttgatag
     3061 taaagtgact caatgttttt aataatttag atttttaata aatgaatatt aattaataat
     3121 ttaatgcaaa ttcaatataa aggagacaat aatatgaata atgacagaga gaaaaacatt
     3181 aatgagacaa ataataatga aagaagcgca accagaccac aattccctaa acgtgcggta
     3241 ataactgcag gaatgcctta tggaaacaaa gagcttcact ttggacatat aggaggggtt
     3301 ttcgttcatg ctgatacttt tgcacgtttc atgagggatc gcattggaaa agaaaatgta
     3361 atctttgtat ccgggacaga ttgctacggt tcaccaatat tggagcatta caaccggttg
     3421 gtcgatgaag gcttgtttga aggcacaatt gaagactttg tacggcataa tcatgaactg
     3481 caaaaagagg ttctgcaatt atatcatatt gatctgaatc tttttgctgc ctcaagtatt
     3541 ggacgtgctg ccgagattca tcgtgagaat tcttctgatt tcattagaaa actatattca
     3601 aatgggcatc ttacaaaaat gacaacttca caattttatg atgcggaact tgatgttttc
     3661 cttaatggcc gtcaggttat tggacaatgt cccattgacg gatgtacatc agaaagagga
     3721 tatgcagatg aatgttcatt agggcatcag tatatgccca ttgatcttat caatcctaaa
     3781 agtactcttt caggagaaaa gccggaaatg agggatgtaa caaactggta ttttaaactt
     3841 gacaaatttc acgaattgtt gtcacagtgg gtggaaatta tggaagaaga ccccaaatcc
     3901 aggaattttg ttttaaaaag tatcaaagag ttcctggaac cgccagtaat ttatgtaaag
     3961 agagatcagc ttgatttgct gaatacaatt aatgataaac tcccggtatt tactttgaat
     4021 gatgaggata ataaatcatc agttgttatg atatttgata acctggagga tagggaaaag
     4081 gcatgtacag tacttgctga gaactcaatt cgtttccgga caggcaaaac tctggtaccg
     4141 ttcagactaa ccggaaatat tgaatggggg gttccggcac catcacttga aggattagat
     4201 gatttgacta tctgggtatg gccggaatct ctatgggctc ctatttcatt tacaatgacc
     4261 tgcctcgaaa tgcagggcaa ggacaagagt acctggaagg cttggtggtc ttcaaagaac
     4321 tcaaaagtat accaatttat tggtgaagac aatgtatact tttatggacc tgcagaaatg
     4381 gccatgttca tgggacagca aggcaaagaa ccgtctgtta acccgccgga gggataactg
     4441 cagcttccgg agctaatagt taacaaccat attctctttt tagataaaaa agccagtagc
     4501 agcggtaaaa tcaaaccacc tgctgctaaa gatctgcttg aatactatac agccgagcaa
     4561 ttaagggcac attttcttgg cttaggtctt ggaataagga gtgtaggatt taaaccaaaa
     4621 ccattagacc ctaaggctgg tgaaaaggat ggtgacccgg tattgaaaga gggcaatttg
     4681 cttgccaatg tatttaaccg ggctgctaga ccatgctttt ataccgcaca aaagtattat
     4741 aatgggaata taccggttgg caatgtaagt gttgaggtac ttgaggaggc tgaaaaaact
     4801 atccttgaat ttgaaagatt aatttcgaaa tatgatttcc atatggtaat gaatctcatg
     4861 gatacgtata ttcgtaatat aaataaatat tggtcaaaaa atatccggca ggcagaagac
     4921 aacaatgatt ccgaactccg ctttcaggta ttggttgaca cttttcatat ggtcagaaca
     4981 gcaacagttc tcatgcatcc tattgctcct gaaggaactg agatgatact tgagtatctt
     5041 aatcttgata aaaatgccga ggaattttgg agctgggaat atgtttttga acctgtatat
     5101 gtttttatga gcaatcctga aaaacataag ctcaaattcc tggaaccaag agttgatttt
     5161 tttaaaaaac atcccagtca gattaagaat attgagcctt agtaattctg tcataattgt
     5221 tggaattttg gtaaaggcat tggaactttg acaaaattac gtatttatgt atattttagg
     5281 taaagacatt aaatatatat ttaaagtatg catattgtaa tatatgaaca aactaacaat
     5341 tgaggacttt gccctggaaa catataaata aataatgcat aaatagatta tatataaata
     5401 aattatataa aggagatgaa tgtatggcaa ttttcgagaa cataggtaag aaagttactg
     5461 aagctgcgca ggttgcggct aaaaaatcgg gagaattagt agaagtaacc aaattaaaca
     5521 tgaatatcag ttcggaagag gatagaatac aaaagcttta tgagcaaatt ggcagggaaa
     5581 tatataaata ttttacttct gaaaaggata ttcctgaaga actaaaagat gtgtgcgctg
     5641 aaatacgatc aagagaagaa actattaaag gtattaaggc aaggattctt gaaattaaga
     5701 atctgcaaga atgcaatgcg tgtggagccg aactggagag aaatattgca ttttgcccca
     5761 agtgcggtgc aaagcaggaa cactaataaa atatgtaccc ggattacttc aacacgaaaa
     5821 acttgtacat caaaaaggct atttccacga aaagggaata gccttttgcc atcggcattt
     5881 tcatcaacag cttttatcta tccaattcaa atcagtagga ctcctgctgt attttccaaa
     5941 aacaaattca aaaaaatttt aaataaaaaa ggaatttaga aatttatgta gaatataatt
     6001 aatagatatg taatcgtgtg catgactatt aatactactt tgttatgctg ctaatggaag
     6061 acattgcttt ccaaattggc agtagtatta atttttatat ttaattaagt agaatgtctg
     6121 ttgaaacaag gagatgttta tgtcagttac tgttaaggat gttgcaagga tggcaggggt
     6181 gtcaactgca acagtttcaa gagtaataaa tgatgatccc agaataagca gggagacaag
     6241 tgaaaaagtt aaaaagtgca ttagtgatct ggattatagg gttaataata tagcaagaag
     6301 tttaaagacc agtaggacat atactatagg gtttatagct cctgagataa ccaatgattt
     6361 ttttatgagc atagccaaag gggtagagga cgaatccaga aagtatggat atagtattat
     6421 tgtatgtaat tcaaatgaaa gtataaagga agaagaagat agaataaggc ttttgagtga
     6481 aaaatgtgtg gatggtatta ttattatacc tgcatcagat gaagggaagc actttaatca
     6541 tttgaaagag cttaatatgc ctgtagttct tgtggaccgg cttgttaata attttgagag
     6601 cgatgccgtg cttgtagata atataaatgg tacatattct gcaatagaat atcttataaa
     6661 tatgggacat ggaaggatag gctttataag cggagattta aaattgacat cggctatgga
     6721 acgctaccaa ggatataaaa gggctttatc agactattgc ataccttatg atgagaaaat
     6781 agtgaaattt ggtgactttc atattagggg cggatatgat atgatgaaaa aactttgtga
     6841 ggctgataac ccgccatctg cagtctttat atcaaactac tttatgcatg taggagctac
     6901 taagtacctt atggaagaag atattaacaa gggaaaaaga agttccgggc agaataacaa
     6961 gaatataata attgccagct ttgatgatat ggaactatct actttactag ggttttgtaa
     7021 attaagagta gcacagccca tgatggaaat tggcagtaaa gcggcacaac ttcttttagg
     7081 tagaattaat aatgactttg aaagaattaa taatgataat gatgctgcag ccggcgacag
     7141 tcagaacaac agagtaataa ggttgaagac cagattggta gtaaatcagc aaattatcag
     7201 ttaatcctga tatggtgtgt taaatcacag aaacaacagg tgagtagtaa ataaaccatt
     7261 aagctgtcca tgacttaagt tcctggctta tagttttgaa aaccggatta aatgcaatcg
     7321 agtgcataaa ctcgaaatta aaaaataaat ttttaagggg gtttttccat ggcaaatcca
     7381 ttaagtatta atgtcaaccc acccaggcaa agactaagag gcagtcttcc caaagtaggg
     7441 atacgcccgg ctatcgatgg caggcgcaga ggtgtgagag agtctcttga ggagcagaca
     7501 atgaatatgg caaaagcagc agcaaaattg attacagaaa atcttaagca tcctaacggt
     7561 atgcctgtag aatgtgttat tgcggatacc tgtattggtg gagtagctga atcagcaatg
     7621 gcagctgaaa agtttgcaag ggaaggtgct ggtgtttccc tcactgtaac tccatgctgg
     7681 tgttatggtt cagaaactat ggacatggat ccctttactc ctaaagccat atgggggttt
     7741 aatgggacag agcggcccgg tgcagtgtat cttgctgccg cacttgctgc tcatagccag
     7801 aaaggactgc ctgcattcag tatttatggc agggatgttc aggatgcaaa tgacacaagt
     7861 atacctcagg atgttagaag aaaaatactt cagtttgcaa aagcagggct tgctgttgct
     7921 gaaatgaaag gaaaatccta tctatctctt ggaacagttg caatggggat tgcaggttca
     7981 atagttaatg aagaattctt ccaaaattac cttggtatga gaaatgaata tgtggatatg
     8041 gtggaattta tcagaagagt agatgaagaa atctatgata aagacgagtt tgaaaaagct
     8101 atgaaatggg ttaaagaaaa ctgtaaggaa ggttgtgata ataaccctga agctgttcag
     8161 cattccagag aaagaaaaga aaaagactgg gaaatgagtg ttaaaatggc tataatagcc
     8221 agggacctta tgattggaaa tcctgaactt gctgatatgg gctatggtga agaggcactt
     8281 ggacacaatg ctcttgctgc aggttttcag ggacagagac agtggactga tcatttcccc
     8341 aacggggatt ttatggaggc catacttaat tcttcttttg attggaatgg cataaggcag
     8401 ccttttatat ttgcaactga aaatgacagt ctaaatggtg tagctatgct gtttggacac
     8461 cttttgacca acacagctca ggtatttgcg gatgtaagaa catactggag tcctgaagct
     8521 ctaaagagag taaccggcat gaagcctgaa ggtcttgcag ctaatgggct gattcactta
     8581 attaattcag gagctgccgc cctggatgga acaggtgaac agacgattaa cggaaaacct
     8641 gcaatgaaac ctttctggga aataactcct gaagaagctg caaagtgcct tgatgcgaca
     8701 caatggagac ccgcaagtct gggatacttc aggggaggcg gatattcatc ggatttcctt
     8761 accaaaggtg gaatgcctgt aacaatgtcc agagttaaca taataaaagg tctgggtccc
     8821 gtacttcaga ttgcggaagg ctattcagta gaacttcctg aaaagataca tgaaatactg
     8881 gataaccgta ccgatcctac ctggcctact acctggtttg cccccatatt aacaggaaga
     8941 ggtccttttg aggatgttta ctctgtaatg gccaactggg gagcaaatca tggtgcattt
     9001 agctatggac atattggtgc tgacttgata actcttgcct cgatgctaag gatccctgta
     9061 tccatgcaca atgtttcaga aggtagaatt ttcaggccaa gtacctggtc agcttttggt
     9121 acttgtgagc ttgaaggagc agattacagg gcatgtgcca atttcgggcc tttatacgga
     9181 agaaaataaa taagaagaaa ataagtaatt agatgtgtat aaaaatgtga tgagatgttt
     9241 tggttactgc tgattttgta acataaattt aattcgggga ctatgattta acccccggat
     9301 tatttttttt acatcgaatt tatataaaca ggcattgatt taaccaaagc gccaaaaagc
     9361 ctcccgcctt tattacagac aggagctgag ttgctgcaga tcggactggg ttgttatttt
     9421 caaaatgcca ggtttgtacg atgcctatat ttgctttaac cggtttttag tcggagatat
     9481 agctcctggg tgcatttaat ttatctgaag aaaatattgg taaggggcac cgtaacgata
     9541 gcccctgata aatatttctt cagatttgat aaatgcaccc tagctcctgc actttagtgc
     9601 agtggtcttc aggtagtgct ataattaata taggtatatg gaagttagta atgcatgaac
     9661 agggaatttt tggaggctga taatgaagta tacttcaatt aatgaaagag tgcaggatat
     9721 taaaggcaat actgaagaaa taagcaggtt tattgaagag tataaaccat ttattgcatc
     9781 atccgctgga aaaatttccg gaaagtatct aaggtacggt gatgacgatg agcttagtat
     9841 agcacttatg gcttttacag aaagcattaa ttcctatgat atttcgaaag gcaaattctt
     9901 aacttttgca caaggagtta taaggagaag gttaattgat tactacagaa aagagagaaa
     9961 acatataaat gttatttctt tgaatgaata ttactatgaa aatacagatg atatggatct
    10021 gagtaccgaa gagtcaatag gaaagttcca gctggatgaa attaatgaat ataggaaact
    10081 tgaaatagca gaacttgata aggaactgaa agggtgggat atcagttttt cggatcttgt
    10141 gaaggtatcg ccgaaacata aggatactcg cgaaacatgt aaagtaataa ttaagcatat
    10201 tctttcaaaa cctgatcttt tagaatttat taaaaacaag aaatacattc ctgtttcaga
    10261 tttaggaaaa tcgttaaatt tatcccgtaa aaagattgat agactccgaa aatatataat
    10321 agcaatgtta attattatta gcggagatta ccaatatcta aaagaatata ttagagatta
    10381 catactttga tttatatgga ctatatataa tccatttaat atttagtata gtaaatctca
    10441 cagtggggtg attttatgaa agggatagtt gtagaaacaa aaggtaggtt tgctgttgtt
    10501 atgaaaaatg atggcgaatt tgtgaaaatt agaaatcacg gaaatctaat agttggatat
    10561 gaaacggata tttccgaacg aaatacagaa tttgttaatt cggctacaac cagacatgcc
    10621 ggtattatat acagaatggc ttctgttgct gctgcactga ttcttatttt tggaatgagt
    10681 tatgtcgttt acagctataa tgtaccatat gcatatatta gtgttgatat aaacccaagt
    10741 atagaaataa ctactaatat atttgataga ataatagaca taaaaggagt taacactgat
    10801 ggagaaaaac tgatagccct tgaagattat gtaaacaaaa aaattgataa gggaataggg
    10861 catataatta acagcgcagt taatgaaggg tatattaagc ctgagaatgg taacatggtt
    10921 tttatgactg tatctgcaaa gaatgaaaaa aaagccggca aacttgaaaa gaatatagaa
    10981 gctgtggcat tagaggaaat tcaagcatca ggagctgatt ccagtctcta tattgaggaa
    11041 gttgcagttg ataaacatga tgaagctgaa attttgggga tatcgccggg taaacttgta
    11101 ttaattgata agttgaggga aagtgattct caaataaaac caagtgacta tgctgatgta
    11161 tctgttaagg aaattatgaa atctgttatt gaaaacaaga aggaagaaat attccaaaat
    11221 aaaatgctga agaatgaaga aaaggccaaa aagagagagg aacatgaatt agagaaagaa
    11281 gaaaacaaac agcagggaag catacaacaa ggaaacaaac tgaaggaagg caagcagcaa
    11341 gataagcaga acaatccggt actgaatgca aaaatgaaaa acaataataa tgagaaacca
    11401 aagaatgaac caaagaatga accaaagaat gaagcaaaga atgaagcaaa ggatggagca
    11461 aagaatccgg taaaaggcaa tcctaacaga aagaatgaag acattaaaga ggataacagt
    11521 gaagatataa ataggcacag cgatgatgat gatattaatt ctattgaaga tgacgaaaaa
    11581 gatactgatt attcaggctt taataaagaa aaagtcaata agggtaatat aaaaccaaac
    11641 gaaaaagtta acgaaaaacc aaacgaaaaa gttaatgaaa aagttaatga gaaagttaaa
    11701 ggaaaaggaa ataaaaaatc aaacgaaaaa ggaaatgtaa aaaaataagc gagaacaaga
    11761 ttaaaagaag aaagatttaa gctatcacaa atagctttaa tttccctact agtagcattc
    11821 tgatgtgcat cagggtgcta ttttttatgc ataagcgggg acataaacag gtaagcaggg
    11881 acagttctcc agttattcat aaaatcttta tgagcataat cgacaaagca tatcataaat
    11941 tttacatttc gaagttaaag tatactacgc ctactacctg atataaatta gactatggtt
    12001 atctactcaa gtcttttggt ggtttatgtc tttaaaaatg ataagaatgc tgactgaaaa
    12061 cgacaaatat cagacattgt attgtttata attggcatta catatttcat actcagagaa
    12121 ctgcg
//
LOCUS       DAOSDD010000133        12123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2034_length_12123_cov_5.344133, whole genome shotgun sequence.
ACCESSION   DAOSDD010000133 DAOSDD010000000
VERSION     DAOSDD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_12123_cov_5.344133"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="PK733_11970"
     CDS             complement(<1..1644)
                     /locus_tag="PK733_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ11293.1"
                     /translation="MKDYTVGKLRNVCLLGHGGSGKTTLAEAMLFNTGVLDRFGKVPD
                     GTTTTDYDPEEIRRKFSINTAMAPCEWNDNKINIIDTPGFFDFVGEVKQGIRVAESAI
                     ISLPAKGGVAVGTEKSWAFAKEQGLAKMFFISKMDEEHANFFEVFDQLVSTFGSGIVA
                     FQIPIIENEKFVGIVDVITRKAGRFDKDKLVDSDIPANLKDKVEELWEGLKEAVAESD
                     EELMEKYFSGDDFTPEEIARGLRNGICQGLIVPVFCGSAINNAGIKLFMDAIVNYLPS
                     PSDTKSITAKKVGSEETVELKPTSDETFSALVFKTIADPFVGKISIFRVYSGTIKTDS
                     VVYNSTTEKTEKVSQLFVLRGKKQIPTDKLIAGDIGGVAKMQNANTNDTICDQSKPVV
                     LEKIVFPEPAISLAVEPKSKGDEDKIGTGLQRMQDEDPTFKVAINSETRQTLIYGMGE
                     QHLDVIISKMKAKFGVGVNLIDPKIPYRETIRKKVKAEGKHKKQSGGHGQYGHVFVEF
                     EPGENDDLTFEEKIFGGSVPRQYFPAVEKGLRESILKGVL"
     gene            2274..3314
                     /gene="aroF"
                     /locus_tag="PK733_11975"
     CDS             2274..3314
                     /gene="aroF"
                     /locus_tag="PK733_11975"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ11294.1"
                     /translation="MIIVMKPGSQEKDIRNISEVLESLGLGVHISKGVERTIIGIIGD
                     KRALRDIPLELMPGVEKLVPIVESYKLASKTFKPEHSTINVNGVKIGGSELIMIAGPC
                     AVENSKQIMEAAFAVKKSGARLLRGGAFKPRTSPYSFQGLEEEGLKMMKEAGDATGLL
                     LVSEVTSEKSLEISEKYVDIFQIGARNAQNFQLLREVGRSKKPVLYKRGPSTTIEEWL
                     NAAEYIMSEGNYNVVLCERGIRTFETATRNTLDLSAVPVVKNLSHLPIIVDPSHAAGK
                     AQYVSSLSLAAIAAGADGLIIEVHPEPRCALSDASQQLTPSDFDILCKDISKMAPIVG
                     REYEIPEVRSEK"
     gene            3498..4619
                     /locus_tag="PK733_11980"
     CDS             3498..4619
                     /locus_tag="PK733_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOJ11295.1"
                     /translation="MKYNLKYLENLKFAVIGLGLMGGSLVKALTQRLGIPDITAVDKN
                     QDSIAAALEDRCISRGFTAVNDQILCADVIFLCTPVKYTLDYVNQLHGKVKPGCIITD
                     VGSTKEQIISHVNNLHNPPCFIGGHPMAGSERTGYVSGSSNLFENAYYVLTPAKSANK
                     ESLEVMVEIINLIGAIPVVIEAHIHDRVTAGISHVPHIIASALVNMIEQIDSPDGKMR
                     MLAAGGFRDITRIASSSPEMWQNIILSNKQHIKEILHIFIEHLEEFLMYLDKIQDVQI
                     MNFFESAKDFRDTFVTNKKGAISPIYEITVDVADKPGIIGEIATILGRNGINIKNINV
                     SNSREFEQGCLVISLPDNESVDKSLNLLSSARYKVFIRN"
     gene            complement(4640..5287)
                     /locus_tag="PK733_11985"
     CDS             complement(4640..5287)
                     /locus_tag="PK733_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOJ11296.1"
                     /translation="MGDIKKISIAVIRRLPRYYRYLSDLLKMDISRVSSKELSQRMGI
                     TASQIRQDLNCFGGFGQQGYGYNVESLYAEIGNILGVNKAFQTIIIGAGNMGQALSNY
                     ENFEKRGFKLIGIFDDNPNLVGREIRNIEIKSMDSLDDFVKYNKVDIAILTVPYQHTP
                     EVAEKVASLGIKGLWNFSAMDLKLPYDDIIIENVHLSDSLMVLGYRLKEAKRLKD"
     gene            complement(5390..6211)
                     /locus_tag="PK733_11990"
     CDS             complement(5390..6211)
                     /locus_tag="PK733_11990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ11297.1"
                     /translation="MIVVLKKSNIALVGLIFILLLAIYSLNINGGSKTAGNIPGELEE
                     VSGKDGIGSIGDSTEGKKAITVTANDSKPKTVILDPGHGGEDPGAVSEYSGLREKDVT
                     LKIAFKVKELLEKENYKVIITRTEDILQYEEGTTDIYQKRKQDLSRRKSIMDDSGADI
                     VVSIHLNKFPETQYYGAQVFYPPESPESRKLANIIQSTLKEKVDPSNKRVAMEKTPPQ
                     GQKPIMILRDVKTTTVIVECGFLSNLEEEKKLGSGEYQDILAAAIKDGISMYFND"
     gene            complement(6335..7537)
                     /locus_tag="PK733_11995"
     CDS             complement(6335..7537)
                     /locus_tag="PK733_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11298.1"
                     /translation="MEILNNDFNREQILEKVGMIIDSFNSLFNESLDKFIDEWEKQTK
                     KKTKKLPGRHKELYDFEDELLSDYNIDKFYEYRDTIYEEWKISPKEELGKVSASDFIN
                     NINDFDLLIEIFLLFAVKGDYDEPEFLLQKLYSFGDKAVDRILDFALDKSLLSYKDSE
                     ENYLISLSSIKALGEWKEQKAITPLINFLLNLPDIISEVTNNENNCDEKSIHMNSQCN
                     EINKFDKHSNREDCEDCEDYENCGDCEEYEKDEMVDLFGEVIRDALVSIGENAIRPLI
                     DILTNTNEYGEPHEYLAMALADIGKDNRSDEIYRCLKKMFLKYDKDKKNITSDCLAVY
                     GDGRAIPALRGYIEKNLDRIDEDIFYAVKIAIDKLGGNMDDIVFYKMDNDDDNDDGND
                     DDGDDYMR"
     gene            complement(7864..8484)
                     /locus_tag="PK733_12000"
     CDS             complement(7864..8484)
                     /locus_tag="PK733_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11299.1"
                     /translation="MLKNHVKGGLKVLGDYAAALLIYFILLYTFIAITKDKFSDWLPL
                     YSFFMFMFMALILYNDMWSLAVKEKKPQYELNPYPLKGLVLGFIGFLPFIILEIVAYF
                     LNFNEPVLNNLKSAILQNLLLGPLYFFINLLGKTTLAYAAASLVIPVLSMISYMLGFY
                     GIEIRKTMGLKKEVTVERKNELSPWNPTRQNTGDGKKKKKNPKKNV"
     gene            complement(8561..8857)
                     /locus_tag="PK733_12005"
     CDS             complement(8561..8857)
                     /locus_tag="PK733_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ11300.1"
                     /translation="MIDLKIYEIKAEKLKALAHTHRLCIVRGLIENQCSVTEIQKCLG
                     LPQSTVSQHLAKLKSAGIIEGTRNGLEVCYKVVDEEVINILKNLFPFQNRDSIL"
     gene            complement(8960..11581)
                     /locus_tag="PK733_12010"
     CDS             complement(8960..11581)
                     /locus_tag="PK733_12010"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HOJ11301.1"
                     /translation="MALKVVIVGGVAGGASAAARLRRLNEDAEIIMFEKGEYISFANC
                     GLPYYIGEVIKEKEKLVVQTPQGMRERFNIDVRVNSEVVKINTESKTVEVFNHTENRR
                     YIEAYDKLVLSPGAEPVKPKLPGIDSPRVFTLRNIPDTYRIKEYVDAMNPKRAIVVGA
                     GFIGLEVAENLHMRGVKVTVVELADHVIGPLDYDMAAIVHQHMKSKDVEFYLKDAVKS
                     FMDEGPCTVVELTSGRFLKADMVILGIGVRPESRLAAEAGLKLGVTGGIFVNEFMQTS
                     NKDIYAVGDAVEVKDYISGNPALIPLAGPANKQGRIAANNICGIGEEYIGTQGTSIVK
                     VFDITVAITGNNERILQKNGVDYEKSFTHSPSHAGYYPGAIPMSIKLLFGKNDGKLLG
                     AQIIGYEGVDKRIDVISTAIRAGMTVYDLEKLELAYAPPYSSAKDPVNIAAYTAANIL
                     KGDCRIFHWNDITSIDKNKSILIDVRTKTEHDLGTIDGAINIPIDELRDRLDEIPKDK
                     DIYIFCQVGLRGYVAYRILTQKGYKSVKNLSGGYKTYQLAVQKQSNEDIYEYDKITKD
                     DMMKPVEKPVEKPADCCGKEEGIKKTAVSCENRDGCKTNIHNNVNVGTKANTDTKTDA
                     DAKANADIIVDACGLQCPGPIMQVYNAIKSMNYGEVLEIKATDPAFQQDVKVWCQRTG
                     NKLLSIGFDNNAFKALIRKEQLSEVNDTDSVKNNKTNDKTMVIFSNDLDKAIASFIIA
                     NGAAAMGRKVTMFFTFWGLNILRKPEKVKVKKDLISRMFGFMMPRGSKKLSLSKMNMG
                     GIGAKMIRHVMNNKNVSSLEDLILQAKANGINMIACNMSMDVMGIKKEELIDGVQIGG
                     VASFLGSAEESDMSLFI"
     gene            complement(11712..>12123)
                     /locus_tag="PK733_12015"
     CDS             complement(11712..>12123)
                     /locus_tag="PK733_12015"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOJ11302.1"
                     /translation="LADICDLYKRILEISEILTSDERFKDLLTSLTRVFNLASKASDV
                     ETDTSIFENESEHSLYSTNFIVKKAVEHKIKLRCFTDAVKSLYELIDPINAFFESTMV
                     IVDDDIIRNNRLSLLKTIADTALQICDFTKIIAM"
BASE COUNT         3639 a         2472 c         1881 g         4131 t
ORIGIN      
        1 caaaactcct tttaatatac tctctctcag acccttttct acagccggga aatactgtct
       61 tggcactgac ccgccgaata tcttttcttc aaaggtgaga tcatcgttct ctccaggttc
      121 gaattcaaca aatacatgtc catactgccc gtgaccacca ctttgttttt tatgctttcc
      181 ttcagcttta accttttttc ttatagtttc tctgtaaggt attttggggt caattaagtt
      241 tacaccaaca ccgaatttgg ccttcatctt gcttataata acatccaaat gctgctctcc
      301 cataccataa ataagtgtct gccgtgtttc agaatttatt gcaactttaa atgtcggatc
      361 ttcatcctgc attctctgca agcctgtacc tattttgtcc tcatcacctt tagactttgg
      421 ttcaactgca agacttatag caggttcagg gaaaacaatc ttctctaata ctacaggttt
      481 tgattgatca caaatagtat cattggtatt ggcattctgc atttttgcaa ctccgccaat
      541 atctcctgct atgagtttgt ctgtaggtat ttgtttcttt cctctcaata caaagagctg
      601 tgataccttt tcagtttttt ccgttgttga attataaaca acagaatctg tctttattgt
      661 accggaatat actctgaaaa ttgatatttt acctacaaac gggtcggcaa ttgtcttgaa
      721 taccaatgct gaaaatgttt catcagatgt aggtttcaat tcaacagttt cttcactgcc
      781 tactttttta gcagtaattg attttgtatc agaaggtgaa ggcaaataat ttacaatagc
      841 gtccataaaa agctttatgc ctgcattatt tattgctgaa ccgcagaata caggaacaat
      901 taaaccctgg catataccgt ttcgcaaacc tctggcaatt tcttcaggag taaagtcatc
      961 tccggaaaaa tatttttcca taagttcttc atcagattct gcaacagctt cttttaaacc
     1021 ttcccaaagt tcctcaactt tatcttttag atttgcagga atatcactat caaccagctt
     1081 gtctttatca aatcttccag ctttccttgt aattacatcc actattccaa caaacttttc
     1141 attttcaata attggtatct ggaaagcaac gataccactg ccaaaagtgc ttaccaactg
     1201 atcaaaaact tcaaaaaagt ttgcatgttc ttcatccatt ttgctaataa aaaacatttt
     1261 ggccaagccc tgctctttag caaatgccca tgacttttca gttcctactg ctactccgcc
     1321 ctttgcaggt aatgaaataa ttgcgctttc agcaaccctt attccctgtt ttacttcacc
     1381 tacaaaatca aaaaatcccg gagtgtcaat aatgtttatt ttattgtcat tccattcaca
     1441 aggagccatg gcagtattaa ttgaaaactt tctcctaatt tcctcagggt cataatcagt
     1501 tgtggttgta ccatccggta cttttccaaa cctatccaat acgcctgtat taaaaagcat
     1561 tgcttcggct aaagtagttt tgccagagcc tccatggccc aataaacata cattacgcag
     1621 tttcccaact gtataatctt tcattaaaac aactccccct taatcatata tttaaatgtc
     1681 cgggacaaca tttcagaaat caggcaacat aaaacagcaa ttaatacttt attgctgcaa
     1741 ccaagccaaa ttaatttggc tatatgagtt taaccaaaat taaggtttaa ccacttccct
     1801 gttttccgta aactgctttg cctaccgtac tttatttatt aattaaacaa attattaata
     1861 tatatatagt atagctattt atgcttgata tttaaacatt attaagcaaa ataatttaac
     1921 atttccatat taaaatttac ctatactaca atatgataac atataataaa ctaatatttc
     1981 aaaatataac aaatttattc ctatcaaagt attcatttaa acagccattt tattatagca
     2041 cataaataat atttttgaat agtgtttttt aaacatttat ggattttatg gattttggat
     2101 tttctaaatt cttgacattt atccgcacca ggtgtaaatt gttatttaat attatttatg
     2161 ataatattga agaatggtag aatagtagaa tagtagaatg gtaattaaaa aaatgtctca
     2221 gactatttaa tgctgaattt tattgtagaa atgtagaaag gaagaataac cgcatgatta
     2281 ttgttatgaa acccggttct caggaaaagg atataagaaa tatatctgaa gttcttgaat
     2341 ctctaggtct tggagtccat atttcaaagg gcgttgaaag aacaataata ggaattatag
     2401 gtgataaaag ggcattgaga gatatacctc ttgaacttat gcccggagtt gaaaagcttg
     2461 tgcctatagt agaatcctat aagttggcca gcaaaacatt caaacctgaa cacagtacta
     2521 ttaatgtaaa cggtgtaaaa ataggcggct ctgaacttat aatgattgca ggtccatgcg
     2581 ctgttgaaaa ttccaaacag attatggaag ctgctttcgc agtaaaaaaa tccggagcca
     2641 ggttattaag gggaggagct tttaaaccaa ggacatctcc atacagtttt caagggcttg
     2701 aagaagaagg tctcaaaatg atgaaagaag caggagatgc tacaggttta ctactggtca
     2761 gcgaggttac cagtgaaaag tctcttgaaa tatcagaaaa atatgtagat atattccaaa
     2821 taggagcaag aaatgctcag aattttcagc ttcttagaga ggtaggtcgt tcaaaaaagc
     2881 ctgtccttta taaacgcggt ccttctacaa caattgagga atggcttaat gctgcagagt
     2941 acatcatgag tgaaggaaat tacaatgttg tattatgtga aaggggtata cggacatttg
     3001 aaacagctac acgcaacact cttgatctga gtgccgttcc tgtagtaaag aacttgagcc
     3061 acctgcctat aatagtagat cccagtcatg ctgcaggcaa agcacaatat gtaagttccc
     3121 tctctcttgc tgcaattgct gcaggtgcag acggacttat tattgaagtg catccagaac
     3181 cgcgatgtgc cttgtctgat gcttctcagc agctaacccc atcagatttt gatattttat
     3241 gcaaggatat aagtaaaatg gcaccaatag tgggtagaga atatgagatt ccagaagtga
     3301 gaagtgagaa gtgagaagtg aaagttaaga ggtggaaagt gagatgtgag gtgcgagata
     3361 tgagagatga gatgttggag gtagaaagta gaaaaaaatt ttgcagttag tggtgggcag
     3421 ttcaagacaa ggtataaaaa gtaggttatg gaaataattg ttagtgatga tgtgtttgtt
     3481 ggttatggtg tgtagtgatg aaatataatc taaagtatct agaaaatcta aaatttgcag
     3541 taattggatt gggattaatg ggaggatctc ttgtgaaagc attaactcag cgacttggta
     3601 tccctgatat tactgctgtt gataaaaacc aggactcaat tgcggcagct cttgaagaca
     3661 gatgtatttc cagaggtttt acagctgtaa atgatcagat tctgtgtgcg gatgttatat
     3721 ttttatgtac tcccgttaag tatactttag attatgttaa tcaacttcac ggaaaagtaa
     3781 aacccggatg tataattaca gatgtgggca gcaccaagga gcaaataata tctcatgtca
     3841 acaacctgca caatcctccc tgttttatag ggggacatcc catggcaggt tctgaaagaa
     3901 caggctatgt ttccgggtca tccaatcttt ttgaaaatgc atactatgta ttaacaccag
     3961 ctaaaagtgc aaacaaggaa tcacttgaag taatggtgga aattattaat ttaattggag
     4021 ctattcccgt ggttattgaa gctcatattc atgatagagt tactgcaggt ataagtcatg
     4081 tccctcatat tattgcatct gcacttgtta atatgattga acagattgat tctcccgatg
     4141 gtaaaatgcg aatgctggca gcaggaggtt tcagggatat taccagaata gcttcatcaa
     4201 gtcccgaaat gtggcagaac attattttga gcaacaaaca gcatataaaa gaaatacttc
     4261 atatattcat agaacacctg gaggaatttt taatgtatct tgacaaaata caggatgttc
     4321 aaataatgaa tttttttgaa tctgcaaaag attttagaga tacttttgtc acaaacaaaa
     4381 aaggagctat aagtcctatc tatgaaataa ctgttgatgt agctgataaa cctgggatta
     4441 ttggagaaat cgctactatt ttaggcagaa acggaatcaa tataaaaaat attaatgtat
     4501 caaatagcag ggagtttgaa cagggttgcc ttgttatttc cctgcccgat aacgaaagtg
     4561 tggataaatc tttaaatttg ctcagttctg ccaggtataa ggtctttata agaaattagt
     4621 gttctttatt tcattttttt taatctttca acctttttgc ctcttttaat ctgtatccca
     4681 aaaccattaa actatcactt aagtgtacat tttcaataat tatatcgtca tatgggagtt
     4741 taagatccat tgcagaaaaa ttccataagc cctttatgcc cagactggct actttttccg
     4801 caacctcggg tgtatgttga tatggaacgg taagtatagc tatatcaact ttattatact
     4861 ttacaaaatc atctagtgaa tccatgcttt ttatttctat attccttatt tccctgccaa
     4921 ccaaatttgg attatcatca aatattccta taagtttaaa acctctcttt tcaaaattct
     4981 cataatttga aagggcctgc cccatatttc ccgctccaat gataattgtc tgaaaggctt
     5041 tatttacacc taatatatta cctatttctg cgtataggga ttctacatta tacccgtacc
     5101 cctgttgtcc aaatccgcca aaacaattca agtcctgcct gatctgagat gctgtaatac
     5161 ccattctctg gcttagttcc tttgacgaca ccctgcttat atccatcttc aacaaatcgg
     5221 acaggtacct gtaatatctg ggcaagcgtc taattaccgc tatcgaaatt ttctttatat
     5281 cccccattat cttcctcctt tgtgattttt tctatccttc cgctaaaatt atatcatcat
     5341 tgttattttt ttgtcaacgc tcggtttacc tgcttgctta ctcaattgac taatcattga
     5401 agtacattga aataccatct ttaattgcag ccgccagtat atcctggtat tccccgctgc
     5461 caagcttctt ttcctcttcg agatttgaca aaaaaccgca ctcaacaatt acagtagttg
     5521 ttttcacatc tcttaagatc ataattggct tttgtccctg aggtggtgtc ttttccatcg
     5581 ctactctttt atttgaggga tctacttttt ctttaagtgt gctttgaatt atattagcaa
     5641 gtttccggct ctcaggagat tcagggggat aaaatacctg agctccataa tattgagttt
     5701 ccgggaattt gtttaaatgt atgcttacaa caatatctgc ccccgaatca tccattatgc
     5761 tttttctccg gctcaggtcc tgctttctct tctggtatat atcagtggtt ccctcttcat
     5821 attgaaggat atcctctgtc cttgtgatta taaccttata gttttctttc tccagtaact
     5881 cttttacctt aaaagctatc ttcagcgtta catccttttc tcttaaaccg ctgtattcac
     5941 ttacagctcc cgggtcttcc ccaccatgtc ccgggtcaag aataaccgtc tttggcttgc
     6001 tatcattggc tgtaacagta atagcctttt tcccctcagt actatcacct atacttccaa
     6061 tgccatcttt acccgatact tcttccaact cacccggtat atttcccgca gtcttgctgc
     6121 ctccattgat attcaaacta tatatggcta acagcagtat aaatatcaat cctacaagcg
     6181 ctatattgct ttttttaagt actactatca tagtgtagct acaccccgtc cctttaaatc
     6241 aatttattat atgattattc agggacatgg gccatatatg catggaaaat gaatttaaat
     6301 aactatttaa tgcatatact catctctcat atgctcatct catataatca tcgccatcat
     6361 catcgttacc atcatcatta tcatcatcat tgtccatctt ataaaagact atatcatcca
     6421 tatttcctcc cagtttgtct atagctattt taacagcata aaagatatcc tcatcaattc
     6481 tatccagatt cttttcaata taacctctta aagcaggtat agcccttccg tctccatata
     6541 cagccaggca atcggaagta atattctttt tgtctttgtc gtattttaga aacatctttt
     6601 tcaagcacct gtaaatttca tcagatctgt tgtcttttcc tatatcagcc aaggccatgg
     6661 ctaaatactc gtgaggttca ccatattcat ttgtgttggt taatatatct attaagggcc
     6721 gaatagcatt ttcacctata gaaacaagag catcccttat tacttcacca aataaatcta
     6781 ccatctcatc tttttcatat tcttcacagt ctccacagtt ttcatagtct tcacagtctt
     6841 cacagtcttc acgattacta tgtttatcaa acttgtttat ctcattacat tggctattca
     6901 tatgaataga tttctcatca caattgtttt cattattcgt cacttcactt ataatatcag
     6961 gcaggttcaa taaaaaatta atcaaaggtg tgattgcttt ttgttctttc cattctccca
     7021 atgcctttat agaagataga gaaataagat aattttcttc agaatctttg taggatagca
     7081 atgatttatc aagagcaaaa tccaaaatac ggtctacagc tttatctcca aaggaatata
     7141 gcttttgcag taaaaactca ggttcatcat aatcaccttt tacagcaaac aaaaggaaaa
     7201 tttctattaa caggtcaaag tcattaatat tattgataaa atcactagca gaaacttttc
     7261 ccagttcctc cttgggactg atcttccatt cttcataaat tgtatctcta tattcataaa
     7321 atttatctat attgtaatcg cttaaaagtt catcctcaaa atcatataat tctttatgtc
     7381 taccaggcag cttttttgtt ttcttctttg tttgcttttc ccattcatca atgaacttat
     7441 caaggctttc attaaataag ctattaaaac tatcaataat cattcctact ttttcaagaa
     7501 tctgctccct attaaaatca ttatttaaaa tctccataaa tcctcccatt tgagatacat
     7561 attatatatg cgcttattcg aaaatatcct taaagctaac atatttgtat tatatatact
     7621 tgccatgatt aatctgcact atgctgacac ttttaatata tactgcatat acttaacata
     7681 tttcacatac tacatataat tcatcttttt attcacttaa tactacttgt caatgccaat
     7741 ttggggagcg atgtctccca ggataatttt gatagcatcg ggaagatgta ttgcatacgg
     7801 gtcagcaaaa gttaccctcc agaaatgcgc tttccattgg caatataaca aagtagtatt
     7861 aatttaaaca tttttcttgg gattcttttt cttcttttta ccatctccag tattctgcct
     7921 tgttggattc catggtgaaa gctcattttt tctctctaca gtaacttcct ttttcaaacc
     7981 catggttttc ctaatttcaa tgccgtaaaa gcccagcata tagcttatca ttgacaacac
     8041 aggtataacc agcgaagcag ctgcataagc cagagtagtt ttaccaagca gattaataaa
     8101 aaaatacagg ggtcccagca atagattctg tagtatagct gatttcagat tatttaatac
     8161 aggctcatta aaattcaaaa agtatgcaac aatttcaaga attataaaag gcaaaaatcc
     8221 tataaaaccc agaaccaatc ccttcaaggg gtatggattt aattcgtatt gaggtttttt
     8281 ctccttaact gccagtgacc acatatcatt atataaaata agtgccatga acataaacat
     8341 aaaaaacgag tataaaggca gccaatcaga aaatttatcc ttggttatag caataaatgt
     8401 atacagcagg ataaaataga ttaaaagtgc tgcagcataa tctcccagaa cctttaatcc
     8461 cccttttaca tgattcttca gcatgatctt tccccctaaa acaataaatt atagtacata
     8521 ggtacattag atatacactg tttatttact ttatatctta ttatagtata ctatctctgt
     8581 tttgaaaagg aaaaagattc ttcaaaatat ttattacctc ctcgtctacc actttgtagc
     8641 aaacctcaag cccattacgg gttccttcaa ttatacctgc ggattttagt tttgccaggt
     8701 gctgtgacac ggtagactga ggcagaccca ggcatttctg aatctctgta acactgcatt
     8761 gattttcaat gagtcccctt acaatgcaaa gcctgtgagt atgagccagg gctttaagct
     8821 tttcagcttt tatctcatat atctttaagt caatcattaa tttacctcca gcaaaaaagt
     8881 caaaaaacgg gacatatcct atcttaaaag aggtatgccc ccaaaattta atatcatgtt
     8941 ttaatgtata agttcatgtt taaataaata gactcatatc cgattcttca gctgaaccaa
     9001 ggaaagaagc tactcctccg atttgcacac catctattaa ctcttccttt tttataccca
     9061 ttacatccat ggacatatta catgcaatca tattgattcc gttagcttta gcctgcaata
     9121 ttaggtcttc aagggatgaa acatttttat tgttcataac atgccttatc atctttgcac
     9181 ctattccacc catattcatt ttagataatg agagtttctt cgaacctctt ggcatcataa
     9241 aaccaaacat cctggaaatt agatcctttt taaccttgac tttttcaggt tttctcaaaa
     9301 tattcaagcc ccagaaagta aagaacattg ttacttttct tcccatggca gccgctccgt
     9361 tagctattat aaaagatgcg atggctttgt ccaggtcatt actaaatatc accatcgttt
     9421 tgtcattagt cttgttattt tttaccgaat cagtgtcatt cacttcagac agctgttcct
     9481 tccttattaa tgctttgaac gcattattat caaaaccgat gcttaggagc ttgtttccag
     9541 tcctctggca ccatacctta acatcctgtt ggaatgcagg atcggtagct tttatttcaa
     9601 gcacttcgcc atagttcata gatttaatgg cattgtatac ctgcatgata gggcccggac
     9661 attgcagccc gcatgcatca actataatat ccgcattagc tttagcatct gcatcagtct
     9721 tagtatctgt attagccttg gtgccaacat taacattatt atggatattt gttttacagc
     9781 catctctatt ttcacaagag actgccgtct tttttatgcc ctcctcttta ccgcaacaat
     9841 ctgcaggttt ttctacaggt ttttctacag gtttcatcat atcatctttg gtgatcttgt
     9901 catattcgta tatatcctca ttagactgtt tctgtactgc aagctgatat gttttataac
     9961 ctccgcttaa attctttacg gatttatatc ctttttgtgt tagtattctg tatgcaacat
    10021 aacctctgag ccccacctga cagaatatat atatatcctt atcctttggt atctcgtcca
    10081 gtctgtctct tagttcatct ataggaatat taattgcgcc atcaatagta cccaggtcat
    10141 gttctgtttt cgttctgaca tcaataagta ttgacttgtt tttgtcaata gaggttatat
    10201 cattccagtg aaaaattctg caatcacctt ttaaaatatt agctgctgta taagctgcaa
    10261 tgttaacagg gtcttttgca gaggaatatg gaggggcata tgccaattca agcttttcca
    10321 ggtcataaac cgtcatacct gcccttatag ctgtagatat aacatcaatt ctcttgtcta
    10381 caccttcata gcctataatt tgtgctccaa gaagctttcc atcatttttc ccgaacaaca
    10441 gctttattga catgggaata gcgcccggat agtaccctgc atgtgaaggg gaatgagtga
    10501 aggacttttc atagtctaca ccgttttttt gcaatatccg ctcattgttc ccggttatgg
    10561 caacagtaat gtcaaatacc ttgacgatag atgtgccctg tgtccctata tattcttccc
    10621 caataccaca tatattgttt gcggcaattc tcccctgctt gtttgcaggc cctgcaagag
    10681 gtattaatgc aggatttccg ctaatataat cctttacctc aacagcatcc cctactgcat
    10741 atatatcttt gtttgaagtc tgcataaact cattaacaaa gattccgcct gttactccca
    10801 gcttaagtcc tgcctctgct gcaagcctgg attccggtct tacacctata cctagtatta
    10861 ccatatcagc ctttaagaat ctgccgcttg taagttccac aacagtacat gggccttcat
    10921 ccatgaaaga ttttactgca tctttaaggt aaaactcaac atccttagat ttcatgtgct
    10981 gatgcacaat agctgccatg tcataatcca gaggcccgat aacatgatct gccagctcaa
    11041 ctactgttac tttgacacct ctcatatgaa ggttttctgc aacttcaagt cctataaagc
    11101 ctgcacctac aacaatagcc ctctttggat tcatagcatc aacatattct tttatccggt
    11161 atgtgtcagg tatatttcta agtgtaaata cccttggtga atcaattccg gggagtttag
    11221 gcttcactgg ttctgcgcca ggcgaaagaa ccaatttgtc ataggcttca atgtaccttc
    11281 tgttttcagt atgattaaac acttcaacag ttttactctc agtattgatt tttacaacct
    11341 cgctgtttac cctgacgtct atattgaacc tttctctcat accttgaggg gtttgtacta
    11401 ccagtttttc cttttccttg attacctcac ctatataata tggcagtccg cagtttgcaa
    11461 aggaaatata ttcgcccttt tcaaacatta taatctccgc atcttcatta agccgtctga
    11521 gtcttgccgc agcacttgct ccaccggcaa ctccgcctac aataactact ttcaatgcca
    11581 taaagtaacc tccctctcaa acatataaaa taataatatc ttattattga tatattctaa
    11641 ttttacgata tcgttaatat attgtcaata aatatgcgga aattgaaatt agcggtgcac
    11701 attttatata cttacattgc aataatcttt gtaaaatcgc aaatctgcaa cgcagtatca
    11761 gcaatagttt ttagcagaga cagtctatta tttcttataa tatcatcatc tacaatgacc
    11821 atagtacttt caaaaaaggc atttatagga tctatcaatt catatagtga ttttactgca
    11881 tcagtaaagc atcttagctt gattttatgc tctacggctt ttttcacaat gaaattagtt
    11941 gaataaagac tgtgttctga ttcattttca aaaatactgg tatcagtttc tacatctgat
    12001 gcttttgaag caaggttgaa caccctggtc agggaagtca gcagatcctt aaatctctca
    12061 tcagaagtca gtatttcaga tatctccaat attcttttgt ataaatcaca tatgtctgct
    12121 agt
//
LOCUS       DAOSDD010000134        11773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2103_length_11773_cov_5.848097, whole genome shotgun sequence.
ACCESSION   DAOSDD010000134 DAOSDD010000000
VERSION     DAOSDD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2103_length_11773_cov_5.848097"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PK733_12020"
     CDS             complement(<1..769)
                     /locus_tag="PK733_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11303.1"
                     /translation="MDKQKSSKLRRIFGSIRSKFLFFFIAAMSFVLVFAVSLTYKKIY
                     EIITRTNERNARSEIKQISTNLNTLYNDLTRQTDIIMYSDYVKVIGDYDSYTPLNLVY
                     SIKDFYASVREMTINFPYIQSVYIFLKNNYVLCATSENMQRFMEVDMSEYNLIKEKII
                     SQNSRSITFVGGMLAKDFPFLFNLKKYSTESNRSLVSAVRNTKDYIIVVNIYEEQLQL
                     AYAGISEKSSCFVRILDLNGNIISSKNMQELGMPYKNR"
     gene            complement(762..1514)
                     /locus_tag="PK733_12025"
     CDS             complement(762..1514)
                     /locus_tag="PK733_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11304.1"
                     /translation="MYRVMIVEDEKFVRCGLRSMINWESLHMEVIVDAKNGVEAYEYY
                     LQYKPDIILTDLRMPLMDGIELIKKIRTQDKYIRFIIITCLDEFALVQEALELGVSSY
                     ILKHSSGVEEIENKLMNVYNELESEGITDNLCKMTNSQCCHPKFTAAMDYIRENFYKN
                     ISLQSTADYIGLTPNYLGRLFVEYIDETFTDTLNQVRIEKAKELLCDLKLPVYAIAKR
                     TGFSNATYFIRVFKRMAGCTPSEYRMRGMSDG"
     gene            complement(1968..4073)
                     /locus_tag="PK733_12030"
     CDS             complement(1968..4073)
                     /locus_tag="PK733_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11305.1"
                     /translation="MPEFNIEKNANILCISGSNFKFIWSIDKGVLSQIKFYDGIKWND
                     ALSQEAYPCSLPASNLVCFNIYDEKDDFVRITTQHKSEDWIIDAEYEVYIRGYIICNF
                     SIEALRNHAKLDELKVGISLDKDAVFSNWYNIKNNFDSSTWTYDRAISVDFSTDGRKV
                     TNSIDFILESVTVGTEGRTCKKVCEDYEKSKFLGWQLGNAAYLKKGFKYSNRWCVTVS
                     GINSAPNKVRAQRIYHWYGFWPTYPSQDILEEMAEYGCSLLALHMQVFKYIDGAIAID
                     EIELIRTINTAHKLGMKVIFYCQPYLISINSPTYNEYNDCLNDGGLMRWHSLKDTQIV
                     YYEPNSDCDNDELCLRCEKAFEYIKSRVINCYEKYCFDGLYVDFGWPGQAICNDTFHN
                     HERGLYNFYDYLKMLREWRKAIGPDAIMIGHGGGLLVSSDFIEGFDACLTGEAQKDIE
                     PQVIGQQFGLSPTLWAMHRKKEKVFRSKDTIAWLIREGLTPHVGVGIMGTSVLASLDP
                     AYNIPLIALWQMWRAFPVHKAAFYNYLTKKVVSLDNNEVTYSLFVTEEKQVMLLLCNM
                     GGKFSESMPAVGVNINIDTKKLKLPEKMKCWRMKGNTYETFRIAQIDDIQKGIINVPE
                     LGISEFIGFILAVENPPDEMINLVNHLDGRAERLYEIQKRKMTRLLEYDKLIDRFAKL
                     PIANNKKSYEEFMKGRVTE"
     gene            complement(4163..5044)
                     /locus_tag="PK733_12035"
     CDS             complement(4163..5044)
                     /locus_tag="PK733_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11306.1"
                     /translation="MQKSNGERVFSVFNGIFLLFIICTVVFPLAHILSISLSEKQAII
                     NQTVGLLPKGLRFDAYKYIFLKPAFLTSLANTVGLTAAYVFTSILVCVMAAYALSKDF
                     YGKRLVTYIFIITMYFSGGLIPSYLLITKYLKLYNTYWALLIPGLVNVFYIIVIRSQI
                     EAIPPSLSEAAIVDGANEFQVLINVIIPSVSATIAAIGMFLALSMWNMWFPVMIYTNK
                     ESLWTLQYFLRAVIFDKFLTAENTAVTNLFMDATDITPQNFQMAAIVLVALPIVSIYP
                     FVQKYFVKGILVGSVKE"
     gene            complement(5146..6099)
                     /locus_tag="PK733_12040"
     CDS             complement(5146..6099)
                     /locus_tag="PK733_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11307.1"
                     /translation="MEGAVVSKRKVVKMKKNRCIFKKERDIPIYALLFPALFLVIIFN
                     YIPMYGIIIAFKNFSPYKGIMGSEWKGIENFKYFLLDNNFWRVMRNTVVINFYKIIFG
                     FPMPIIFALSLNELIRVKIKKLIQTLSYLPYFISWVVAAGIFIQILSPTGGIINLLMN
                     RFLGIEPIYFLTKKEYFVSIVIISSIWKDFGMGSVYYLATLSTIDPTLYEAAKIDGAG
                     RWKQTVHITLPGIKYIAIVLFIINIGSMVTIGFEQIFLLYNPLVYDVGDVISTYTYRL
                     GIEQTRFSLTTAIGFTQSIVNFVLVYSANKISKKVAGWALW"
     gene            complement(6341..7960)
                     /locus_tag="PK733_12045"
     CDS             complement(6341..7960)
                     /locus_tag="PK733_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11308.1"
                     /translation="MRSKKYICLLMAVVFALSVLFTACSSNTNKNDNDNTQANKSQNT
                     DLGEKAKSAPAKIRFMYSDIGLGIPDDVDPGNNPFLNKIAEQANVEFTEVIRPPYTDY
                     TTKLNLVLSSGDIPDVLFPMWSNADVEKQGMNGAFLPLNDYIAKSRYASMYTEPMIDA
                     MKSADGNIYIWMSVPSGDPSALAGRKDLIDELNNGVIPKSPDDWYQLAKKLKQKYPES
                     VPFSSGFGIPPTFFASYGVNIGASGANWQYTNEKYINAFEAPLCRDSVLFHKKLYDEG
                     LLDRTFVTNKLEDYNERKWNKKLLFVPASYLGVAVYVGHYVRNNVTDAIINPIPLPIV
                     DDPKIDSANVYSGLGLLGGTGLAIASQTKEKEGCVRLIESFLSPETLELCAWGRNGVE
                     HTITDGKKVLNVENSLKTTYRFIYGIMRYYWDPEVSGVRARQEISLLDESVRDKVTKQ
                     FEDVLPTLVENAKKNPAISPDKFVKLSEDTMSRQSEASELSKSIILKAIIGEISMEEY
                     DSQVTAFLKKYSFITDEYNKKLAEVKQKWRY"
     gene            8356..10806
                     /locus_tag="PK733_12050"
     CDS             8356..10806
                     /locus_tag="PK733_12050"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11309.1"
                     /translation="MIRKFTSQVRNLTYSKVPYSDFKNSKFFFSFLTKLLLSYSIVII
                     ICISSMGLLSYKFIGKNIVEKNIKSNNEMLSQFKYVADTYILDNVNQLSIKIMQDLKI
                     NPNISYYLRNPLHGHLVNVLNVDNYLSSVKYASSNISMLGLYYKYNDLAISTDFIRYE
                     YTSNISSNDKLKYLKELIAAGKYYDNACWSFDTNYPYETASQSYNSGNSKYEHTNVIL
                     YIRKIPESISIPNSSGAIIIVMKETVLYNIIKSITPSGIDKIIVIDDEGKIISHSDKN
                     NLGRNIIDYNFGNKILKSGNKSDYFIDNISGISSVISYTTSDYNKWRYITITPMANYT
                     SATGFLTKTIIIISLLTVLLALVISIISARNFSSPVKNLVNYCRQISNNPELKSKNEF
                     FIIKTLLNNMSSLINTQEKRFISSLPLLKQNFVQNMITNNLKDKIEIQKRMEMLEIDF
                     PYKNYYVISALLTSQKDDNIVEYELNKINLKDDICKCFNRKNIRNISTEYNNYLVFII
                     NSELGYQNIIDEILPQIKEYENNSTSKIHMGIGNQVEDMTLLYQSFNQSISALKYRYV
                     YPEKMVFTVMESDKWDNNDNEINCKILKKIIQGIQGVNKKNTLNAFDDLFFCFQQQQL
                     KYSYIINVLIQCISLIESLLSGKNTTINEITGIKEDIYNQFYSNTNVIEAKKWFEGIS
                     IKIIDYFDGKNSAIPDELINKAKEFILENIHRQDLSLNMVANNIFISPNYLSKQFKTE
                     EGVLFIDYVNKLRMDKACELLLSTSYSISKISALTGFSSSQYFIKKFKYTYGMTPNNF
                     RHATVNKVDMQELGLQKK"
     gene            complement(10845..10920)
                     /locus_tag="PK733_12055"
     tRNA            complement(10845..10920)
                     /locus_tag="PK733_12055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10885..10887),aa:Pro,seq:tgg)
     gene            11348..>11773
                     /locus_tag="PK733_12060"
     CDS             11348..>11773
                     /locus_tag="PK733_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOJ11310.1"
                     /translation="MIKEGDRIAVGVSGGKDSLALLVALKDMQRFYPEKYELEAITLT
                     LGIGTPDFSPVEKLCRDLDINYTIEETLIGKIVFEARADQNPCSLCANMRRGALHNTA
                     KKLNCNKVALGHNRDDVIETLLLSLFYEGRIHTFSPVTYL"
BASE COUNT         3977 a         2197 c         1725 g         3874 t
ORIGIN      
        1 ttcggttttt atatggcatg ccaagctcct gcatgttttt cgaacttatt atattgccgt
       61 tcaaatcaag aatacgaaca aaacagcttg atttttcaga aattccagcg tatgccaatt
      121 gtaattgctc ttcgtatatg tttacaacaa tgatataatc tttagtatta cgcactgccg
      181 atactaagct tctattactt tcagtagaat actttttcag gttgaaaaga aaaggaaagt
      241 ccttcgctaa cattcctcca acaaaagtaa tacttcgtga gttttgagag attatttttt
      301 cttttatcaa attatattca ctcatatcca cttccataaa cctctgcatg ttctcacttg
      361 tcgcgcaaag aacataatta ttcttcaaaa agatatagac tgactgtata tatggaaaat
      421 ttatcgtcat ttcacgcact gaagcataaa aatcctttat tgaatatacc aaatttaaag
      481 gtgtatacga gtcatagtcc cctatcactt tcacataatc actatacatt atgatatctg
      541 tctgtcttgt taaatcgtta tagagagtgt ttaaattcgt tgatatctgt ttaatttcag
      601 atcttgcatt acgctcgttt gtcctcgtaa tgatttcata aatttttttg taggtaaggg
      661 acactgcaaa aacaagcacg aaggacatgg cagcaataaa aaaaaacagg aatttcgagc
      721 gtatgcttcc aaaaatacgg cgcagcttgc tgcttttctg cttatccatc agacatcccc
      781 ctcatacggt attcactggg tgtacatcct gccattcgct tgaatacacg tataaagtat
      841 gtagcattag aaaatcctgt gcgttttgct atagcgtaca ccggcagttt caagtcgcat
      901 agcagttcct ttgccttttc aatacgtacc tgattgagag tatcagtgaa tgtttcgtca
      961 atatattcaa caaacaaccg tccaaggtag ttaggtgtga gaccaatata gtcggcagta
     1021 ctttgcaagc ttatattttt ataaaagttt tcgcgtatat aatccatagc agcagtaaat
     1081 ttaggatgac agcattggga atttgtcatt ttacataaat tatctgttat cccttcactt
     1141 tcaagctcat tgtagacatt catgagctta ttttcgattt cctctacgcc ggaagaatgc
     1201 tttaaaatat aactggacac gcctaattcc aatgcttcct gcactaaggc gaactcatcg
     1261 aggcaagtga ttataataaa ccttatatat ttatcctgcg tccttatttt ctttataagc
     1321 tcaataccgt ccatcaatgg catacgaagg tcagtcaaaa ttatatcagg tttgtactgt
     1381 aagtaatatt cgtacgcttc tacaccattt ttagcatcga ctatgacctc catgtgaaga
     1441 gattcccagt ttatcatact cctaagaccg caccgaacaa acttctcatc ttcaactatc
     1501 ataacacggt acattgattt ctacaccccc aatactgtaa tgcaacaaac tataaacgta
     1561 tgcaagcctt actatgcgct ccctaccttt cgtgcattta ttttatcctt attaacagca
     1621 tcagtatatg taatccacat attctgcatt tcacaactaa tccaagctaa tattttcata
     1681 atttaactac ccaatcattc tatcaaatgt tatagtaata ttcaatatgc gttggaattc
     1741 gttgaatttc agtataaaaa agatataata aaactcaaat aggacattca ccgtcaagtt
     1801 atcgtctatg caaagtacgc aaaaaaccat gtaatgcttt tattacatgg ctttgtatat
     1861 cattacacct tattgagtta agactcccat ctctataggt gggacctaaa tcaggtggag
     1921 tagagtctcc atctgattcc ccgatgttca gctttagctg aacgagttca ctctgttacc
     1981 ctgcccttca taaattcctc ataactcttt ttattattcg ctataggaag ctttgcaaaa
     2041 cgatcaatta gcttatcata ttccaaaagt ctggtcatct tccttttttg tatttcatat
     2101 aatcgttcag ctctgccatc aaggtgatta accaaattta tcatttcatc aggtgggttt
     2161 tctacagcta aaataaatcc aataaattca gatattccaa gttcaggcac attgattata
     2221 cctttttgaa tatcatcaat ctgagctatt ctaaaggtct cataagtgtt tcctttcatt
     2281 cgccagcatt tcattttttc aggtaatttt aattttttag tatcaatatt tatatttaca
     2341 cctacagcgg gcatgctctc agagaatttt cctcccatat tgcataacaa aagcataacc
     2401 tgcttctctt ctgttacaaa caagctatat gttacttcat tattatccag agatactact
     2461 ttctttgtga gataattgta aaaggcagct ttatgtacag ggaaagccct ccacatctgc
     2521 cataatgcaa ttagagggat gttataggct ggatcaagag aggcgagcac agaggtaccc
     2581 ataataccta cacctacatg aggtgttagc ccttctctta tcaaccatgc aatagtatcc
     2641 tttgagcgga atactttttc tttttttcta tgcattgccc ataaagtagg tgaaagccca
     2701 aactgctgac ctattacttg tggttcaata tccttttgtg cttcccctgt gagacaggca
     2761 tcaaaccctt ctatgaaatc actagatact agtaagcccc cgccatgacc tatcataata
     2821 gcatcaggtc ctattgcctt tctccactca cgcagcattt tcagataatc atagaaattg
     2881 taaagaccac gctcgtggtt atggaaagta tcgttgcata tagcctgtcc aggccatcca
     2941 aagtcaacat atagtccatc aaaacaatat ttttcgtagc agttaattac tctgctttta
     3001 atatattcga aagctttttc acatcttaag cacagctcat cattatcaca atcactattt
     3061 ggttcgtaat atactatctg ggtatctttc agagaatgcc acctcattaa ccctccatca
     3121 ttcaggcagt cattatactc attataagta ggagaattaa tagatataag atagggttgg
     3181 caataaaaga ttactttcat cccaagttta tgtgcagtat taattgtgcg aatcagctct
     3241 atctcatcaa ttgctatggc tccatctatg tatttaaata cctgcatgtg taatgcaaga
     3301 agagagcaac catattcggc catttcctca agaatatctt gtgaagggta agttggccag
     3361 aatccatacc agtgataaat tctctgggct cttactttat taggtgcact gtttattccc
     3421 gatacagtta cacaccacct gttactgtat ttaaagcctt tttttaagta tgctgcattt
     3481 ccaagttgcc atcccagaaa cttagatttt tcataatctt cacatacctt tttgcatgtt
     3541 ctgccttcag ttccaacagt aactgattct agaataaagt caatactgtt tgttactttt
     3601 ctcccgtcag tgctgaagtc taccgatatg gcacggtcat acgtccatgt actgctatca
     3661 aaattatttt ttatattata ccagtttgaa aatacagcat ctttatcaag ggaaatacca
     3721 actttgagtt catcaagttt cgcatgattc cttaatgctt caatactaaa attacatatg
     3781 atatatcccc ggatgtaaac ctcatattct gcatcaataa tccaatcctc tgacttgtgt
     3841 tgggttgtaa ttcttacaaa atcatctttt tcatcatata tattgaaaca tacaaggtta
     3901 ctagcaggta gtgagcacgg atatgcttcc tgtgataatg catcattcca ctttattcca
     3961 tcgtaaaact taatttggct cagtactcct ttatcaatac tccaaataaa cttaaaattg
     4021 ctgccactaa tacataaaat atttgcattt ttctctatat taaattccgg cattttaacc
     4081 cctccacaaa taatattgct aaaatatcat attcccatac tattattcat tttatctttt
     4141 ttacactcat atttaccatt ctttattcct tcactgaacc aacaagaata cccttaacaa
     4201 aatatttctg tacaaatgga tagatggata ctataggcaa tgctacaagt acaattgctg
     4261 ccatttgaaa attttgaggt gttatatctg ttgcatccat aaataagttt gtaactgcgg
     4321 tgttttcagc cgttaaaaac ttatcaaaaa taaccgctct cagaaaatat tgaagtgtcc
     4381 aaagtgattc tttgtttgtg tagatcatga caggaaacca catattccac atacttaatg
     4441 caagaaacat ccctatggca gcaattgtag ctgatacaga aggaataatt acatttatta
     4501 atacctgaaa ttcattagcc ccatctacaa tagctgcttc cgataggctg ggtggtattg
     4561 cttcaatctg gcttctgata acaataatat aaaaaacatt taccaggcct ggaattagca
     4621 gagcccaata agtattatac aatttcaaat acttggtaat caaaagatat gatggtatca
     4681 agccccctga aaagtacata gtaataataa aaatataggt aaccaaccgt tttccataga
     4741 aatcttttga aagcgcatat gctgccataa cgcaaaccag aattgaggta aacacataag
     4801 ctgccgtaag ccctacagta tttgccaatg aggtcaaaaa tgcaggcttc aaaaatatat
     4861 atttatacgc atcaaatctc aaacctttgg gcagtaaccc tacagtttgg tttatgattg
     4921 cctgtttttc gcttaaagag atacttaata tgtgagctag tggaaataca actgtacaaa
     4981 ttataaataa taaaaaaata ccattaaaaa ccgaaaatac cctttcacca ttagactttt
     5041 gcataaaaaa acctccatgg aatcaaaatg taaatacttc aggcataata aaaagaatat
     5101 ccagtcggag ttcaaccgaa ttatcaaaaa atttgattat gccaattacc aaagagccca
     5161 ccctgccact ttctttgata ttttattggc agaataaact aaaacgaagt tcactattga
     5221 ttgagtaaac cctatagctg tagttaaact gaaccttgtc tgttctatcc ccaacctgta
     5281 ggtataggta cttataacat ccccaacatc ataaaccaga gggttataaa gtaaaaatat
     5341 ttgttcgaat cctattgtaa ccatactacc tatatttata ataaaaagta ctatagcaat
     5401 atactttatt cctggtagtg taatatgcac tgtctgcttc cacctgcctg ccccatcaat
     5461 ttttgcagcc tcatataatg ttggatctat agtggatagt gtcgccaggt aatatacaga
     5521 tcccatgcca aaatccttcc atatgcttga gataataaca atagaaacaa aatattcctt
     5581 tttagttaaa aaatatattg gttctatacc taaaaatcta ttcattaata aatttataat
     5641 tcctcctgta ggagataata tttgaataaa aatacctgca gctacaaccc atgatataaa
     5701 ataaggcaga taactcaatg tttgaataag ttttttaatt tttactctta tcaattcatt
     5761 tagcgaaagg gcaaatataa tcggcatagg aaaaccaaat ataattttat aaaagtttat
     5821 aactactgta tttctcatta ctctccagaa gttgttatcc aataaaaaat atttaaagtt
     5881 ttctatcccc ttccactcac ttcccataat acctttatac gggctgaaat tcttaaaagc
     5941 tataataatt ccatacatag gtatatagtt aaaaataata actaaaaata gtgctggaaa
     6001 taagagggca taaataggta tatccctttc tttcttaaat atacatcggt tttttttcat
     6061 tttaactacc tttctttttg atacaacagc accttccata tctctctcct tctacatact
     6121 tcaacataga aattttgctc ataactttaa gcaatgatgc gaattttctt gttcactaat
     6181 aaggtatggg gacacaccgc tgctacgtag gtgttccttg ttgcagaatg tgccccatcc
     6241 ctaggttccc ttcgacggat tctgctcttt tatctattgc agaatactga gtaccttgta
     6301 tttatattcc aagataccaa accccaagtt aggcttttac ctaatacctc catttttgtt
     6361 ttacttctgc cagtttcttg ttatattcat ctgttatgaa agagtacttt ttaagaaatg
     6421 ctgtaacctg tgagtcatat tcttccatgc tgatttcccc gattattgct ttgagtatta
     6481 ttgatttgga aagttctgat gcctcactct gccttgacat agtatcttca cttaatttta
     6541 caaacttatc aggagaaata gcaggattct ttttagcgtt ttcaacaaga gttggaagaa
     6601 catcctcgaa ttgcttagtt accttatctc taaccgattc gtccaacaag ctgatttcct
     6661 ggcgggctct tacacctgaa acttcagggt cccaataata cctcataatg ccgtatataa
     6721 atctataggt agttttaagg ctgttttcta cattaagtac ctttttaccg tcagtaattg
     6781 tatgttcaac tccatttctt ccccatgcac acaactctaa tgtttccggt gaaaggaaag
     6841 attcaattag ccttacacaa ccttcctttt ccttagtttg tgaagcaatt gcaagacctg
     6901 tgcctccaag aagccccaga ccactatata cattagcaga atcaattttg ggatcatcta
     6961 ctataggaag aggtatcgga tttattattg catcagtaac attattcctt acataatgcc
     7021 cgacataaac cgctacacct aaatatgatg caggaacaaa caacaatttt ttattccatt
     7081 ttctttcatt ataatcctca agtttattcg taacgaatgt tctatccaga agaccttcat
     7141 catagagctt tttatgaaac aaaacagagt ctctgcaaag tggtgcttca aatgcattaa
     7201 tatacttttc atttgtgtat tgccaatttg ctccactggc tccaatattt actccgtaag
     7261 aagcaaagaa tgtaggtggt attccaaagc cacttgagaa cggtacagat tccggatact
     7321 tttgtttcaa ctttttagct aattgatacc aatcatcagg cgattttgga attacaccat
     7381 tatttaattc atctataaga tccttcctgc ctgctagagc tgacggatct ccacttggca
     7441 cggacatcca gatataaata ttaccatctg cagatttcat agcatctatc ataggctctg
     7501 tatacatgct tgcataacgt gatttagcaa tataatcatt taagggtaaa aaagcaccgt
     7561 tcattccttg tttttcaaca tcagcattgc tccacatggg aaacaatacg tccggtatat
     7621 ctccggaaga taaaaccaga ttcagttttg ttgtataatc cgtatatgga ggtcttataa
     7681 cttcggtaaa ctctacatta gcctgttcgg caattttatt caaaaatgga ttatttccgg
     7741 gatcaacatc atctggtata cctaatccta tatcactata catgaatctt atttttgcag
     7801 gcgctgattt ggctttttcc cccaaatcag tattttggct tttatttgcc tgggtattat
     7861 cattatcatt tttatttgta ttagaagagc atgccgtaaa taaaactgat aaagcgaata
     7921 ctactgccat taataaacaa atatactttt tggatctcat aaaacatcct cctgttaaat
     7981 ataatactgt ataaaactac atatttgcat attacttgta aaaccagtta tgaaactata
     8041 agttaaatta aaaagtctaa tctaaaatat taatgcgcac tcaatatttg ttttgagtat
     8101 atcaacttaa aatacaggca tcaatattaa tatttagcta atggtgaaaa taattatcgt
     8161 tttgtcaacc atccatatta tactttttcg ctaaaatgaa aattttttat gcttttttta
     8221 tccctatatt taaaaaagca gtaaagcatg ttataattct tatgtaagca tgcttaagca
     8281 tgttttgcag gtttgctttg ttaatgaatc agcagcttga ttcaaatatt taagctatgg
     8341 agcatagaaa taaatatgat tagaaaattt acatctcagg ttagaaatct tacatatagt
     8401 aaagtaccat attctgattt taagaatagc aaattttttt ttagcttttt aacaaaattg
     8461 ctgttatcat attcaatagt aattattatt tgcatttcca gcatgggctt actttcatac
     8521 aaatttatag ggaagaatat tgttgaaaag aatattaaat ctaataatga aatgctctca
     8581 caatttaagt atgttgcaga tacatatata cttgataatg ttaatcagct ttccataaaa
     8641 attatgcagg atttaaaaat aaatcctaac atttcatatt atttacgtaa tccattgcat
     8701 ggtcatctcg tcaatgtact taatgttgat aattacctat cttcagtaaa atatgcaagc
     8761 tccaatattt ctatgttagg attatattat aaatataatg atcttgcgat ttcgacagat
     8821 tttattagat atgaatatac cagcaacata agcagcaatg ataagctcaa ataccttaaa
     8881 gaactaattg cagcaggaaa atattatgat aatgcctgct ggtcctttga tactaattac
     8941 ccatatgaaa ctgcatctca atcatataat tccggcaaca gcaaatacga acatacaaat
     9001 gttattttat atattagaaa aatacctgag agtataagta ttcccaatag cagcggagca
     9061 attattatag ttatgaaaga gacagtactt tacaatatta taaaaagtat tacaccctca
     9121 ggcattgaca aaattatagt aattgatgat gaaggtaaaa taatatccca ttctgataaa
     9181 aataaccttg gcagaaacat aattgattat aattttggca ataaaatact aaaatccgga
     9241 aataaatccg attactttat agataacatc tctggaattt cttcagtaat atcatacaca
     9301 acatctgatt ataataaatg gagatatatt acaattactc ctatggcaaa ctatacttca
     9361 gctacaggtt ttttaacaaa aaccataata attatatctt tgctaactgt actattagct
     9421 cttgttatat caataatatc tgcaagaaat ttctccagtc cggttaaaaa ccttgttaac
     9481 tactgtaggc aaatatctaa taatcccgag ttaaaatcca aaaatgaatt cttcataata
     9541 aaaacattgc ttaataatat gtcaagcttg atcaatactc aggaaaaaag atttatatca
     9601 agtcttccgc ttctaaagca aaattttgtt caaaatatga taacaaacaa cttaaaagat
     9661 aaaattgaaa tacagaagcg gatggaaatg cttgaaatag attttcctta taaaaactac
     9721 tatgtaattt cagccttact tacaagccag aaagatgata atattgtaga atatgaatta
     9781 aataaaatta accttaaaga tgatatatgc aagtgcttta accggaaaaa tattaggaat
     9841 ataagtacag aatataataa ctatcttgtt tttataataa acagtgaact cggttaccaa
     9901 aatattattg atgaaattct acctcaaatt aaggaatacg agaataatag tacatcaaaa
     9961 atccatatgg gaataggaaa ccaggttgaa gatatgacat tgctttatca atcattcaat
    10021 caatctattt cagcattaaa atacagatat gtttaccctg aaaaaatggt gtttacagtg
    10081 atggaatccg ataagtggga taacaacgac aacgagatca attgtaaaat attgaaaaaa
    10141 ataatacaag gtattcaagg agtaaataaa aaaaacacac ttaatgcgtt tgatgactta
    10201 ttcttttgtt ttcagcagca acaactaaaa tatagttaca tcattaatgt attaatacaa
    10261 tgtatatctt taatagaaag cttactttcc gggaaaaaca caacaataaa tgaaataaca
    10321 ggaattaaag aagatatcta caatcagttt tatagcaaca ctaatgtcat agaagcaaaa
    10381 aaatggtttg aaggtatctc tatcaaaatt attgattact tcgacggtaa gaactccgca
    10441 atacctgatg aattaattaa taaagcaaag gaatttatac tggaaaatat ccacaggcaa
    10501 gacttatctc ttaatatggt ggcaaataac atttttataa gtccaaacta cctaagtaag
    10561 caatttaaaa cagaagaagg tgttttattt atagattatg ttaataaatt aagaatggat
    10621 aaagcatgtg aacttctcct ttctacaagt tatagtataa gtaaaatatc tgcattgaca
    10681 ggattttcct catctcaata ttttataaag aagtttaagt acacatatgg aatgactcca
    10741 aataatttca ggcatgctac agttaataag gttgatatgc aggagcttgg attacaaaaa
    10801 aaataaaaac ttacagaaca atatatcctg taagtttttt aacctggtcg gagtgagagg
    10861 acttgaacct ccggcatctt ggtcccaaac caagcgcgct accaactgcg ctacaccccg
    10921 caaaaaatat attaacacat attaatcgaa aggtcaacag gttttttgtt tacttttttt
    10981 accaccccat tcgtttgaaa tgaatatctc atcagtgaag tacagagcta gaattatcat
    11041 atatacttag ctaattctat actgagaact actgggcact actgggcaca attattacag
    11101 taatgcaaca ttcctgtaat aatcatgtaa tattgtaaca aaacgttaac ttgatactgg
    11161 tttgcgtata ttctataatc tatttaggga aaaatgcagg caaaggaaat atcaaataat
    11221 aaatattgca atacaattaa tactaatgat aaaaaggtaa acaactatga gtactaagaa
    11281 agatgctaaa gcaagaactc gaaaggaaat attgggccag gtaagaagag ccgttcatga
    11341 ctatagtatg atcaaagaag gagaccggat agccgtaggt gtttcaggtg gaaaagacag
    11401 ccttgctttg ctggttgcac taaaagatat gcaaaggttt taccctgaaa aatatgaatt
    11461 ggaggcaata actctcactc ttggcatagg gacaccggat ttttctcctg tggaaaaatt
    11521 gtgccgggat ttggacataa actatacaat tgaagaaact ctcataggga agattgtttt
    11581 tgaagcaagg gctgaccaga atccatgctc tctttgtgca aacatgagac gcggagctct
    11641 gcataataca gcaaaaaaat tgaattgcaa caaggttgca ctcggccaca acagggatga
    11701 tgttatagaa acactactgt taagtttgtt ttatgaaggc aggatacata cattctcacc
    11761 agttacctat ctt
//
LOCUS       DAOSDD010000135        11729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2113_length_11729_cov_5.725715, whole genome shotgun sequence.
ACCESSION   DAOSDD010000135 DAOSDD010000000
VERSION     DAOSDD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2113_length_11729_cov_5.725715"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3019)
                     /gene="rpoB"
                     /locus_tag="PK733_12065"
     CDS             complement(<1..3019)
                     /gene="rpoB"
                     /locus_tag="PK733_12065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ11311.1"
                     /translation="MVHPVYMGRNTRMSYSRIDEVLEMPDLIEIQKNSYKNFLDEGLM
                     EVLRDVSPITDYTGNLIMEFVGYSLDENPKYSIEECKERDTTYAAPLKVKARLINKET
                     GEVKEQEIFMGDFPLMTETGTFIINGAERVIVSQLVRSPGTYYDVKIDKTGKRLYSAT
                     IIPNRGAWLEYETDSNDIIYVRIDRTRKLPITVLVRAMGYGMDLDVMEYLGEDERVLA
                     TIQKDNSKTEEEGLLEIYKRLRPGEPPTVESARQLINNLFFEPKRYDLAKFGRFKFNK
                     KLSLASRITGFVTADNVIDTRTGEIIISADEVIDRATAAKIQNAGINSVYLNVNGKRI
                     RVIGNHTVDIRQYLDLDLSDIGIEENVYLAELGKILAENNTPEDIKKAIKANIDNLLP
                     KNITSDDIMASINYLVNLGYGVGDVDDIDHLGNRRLRSVGELLQNQFRIGLARMERVV
                     RERMTIQDMDIVTPQSLINIRPVAAAIKEFFGSSQLSQFMDQTNALAELTHKRRLSAL
                     GPGGLSRERAGFEVRDVHHSHYGRICPIETPEGPNIGLIGSLSTFARVNNYGFIETPY
                     RRINKETGTVTDEIDYLSADIEDEYIVAQANEPLDKDGRFVNDRVTVRFKNDIFQVEK
                     SRVDYIDVSPKQIVSVATALIPFLENDDASRALMGSNMQRQAVPLIKTQSPIVGTGIE
                     YKSARDSGVVVLAKNGGTVEKVTAKEITIKRADNRRDSYKLLKYMRSNQGTCINQRPI
                     VRKGDVVKAGDVIADGPSTDNGELALGKNVLVGFMTWEGYNYEDAILISEKLVKDDTF
                     TSMHIEEYEAEARDTKLGPEEITRDIPNVSEDSLKDLDDRGIIRIGAEVRAGDILVGK
                     VTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGEQGIIVDVKVFSRENGDELPP
                     GVNQMVMVYVAQKRKISVGDKMAGRHGNKGVISRILPEEDMPFLPDGTPLEIVLNPLG
                     VPSRMNVGQVLEVHLGYAAKALGWKIATPVFDGA"
     gene            complement(3484..4344)
                     /locus_tag="PK733_12070"
     CDS             complement(3484..4344)
                     /locus_tag="PK733_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ11312.1"
                     /translation="MELNMKLKQPGAELFIPDNLTVEEGIARTTHMGISAHQDDIEIM
                     AYHGVMECFGKEDQWFFATILTDGAGSPRDDLYANYSDEDMKQVRKKEQKKAAYVGEY
                     GALALMNYPSKAVKDPNNEDVINELAELIAHAKPKVVYTHNLADKHDTHVGTSVKVIK
                     AIRRLPAEQRPEKLFGCEVWRCLDWINDNEKVLFDVASHQNISAAILEVHDSQICGGK
                     RYDLATAGRRLANATYAESHGTDNTTALTYAMDLTPLIQDESLDIISYVEGFIERFKE
                     DVYNRVKKMA"
     gene            complement(4724..6124)
                     /locus_tag="PK733_12075"
     CDS             complement(4724..6124)
                     /locus_tag="PK733_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ11313.1"
                     /translation="MKSGKIIINPLFTPVLDKNFEPAILANREFIKAVKESGKGVPLH
                     IALERNDGLISTYHTEVFREGTGMDEENFFYVERLLKTLLWMRGGWKIIIGGPVSIGE
                     YISRAYSQGGLREFDAKFMGRVYEKDFTVEITDLDNVPCAKEETQPIGRNFNGNRIGF
                     DAGGSDRKVAAVIDGNAVYSEEVIWHPKTESNPDYHYSEILKAIKTAASYLPHVDAIG
                     VSAAGIYINNRVMVASLFIKVPDDAFDKKVKNIFLDIQKEMGGIPIEIANDGDVTALA
                     GAMELNDTNVLGIAMGTSEAGGYVDCHGNLTGWLNELAFVPVDFNTESMVDEWSGDFG
                     CGVKYFSQDAVIKLAPAAGIELAGNLTPAEKLKVVQKLLHEGHEGAKGIFESIGCYLG
                     YSLAYYAEFYDIKHILILGRVTSGEGGNLILHKAEEVLKGEFPDLAAKVKLHLPDEST
                     RRVGQAIAAASLPEIR"
     gene            complement(6507..6890)
                     /gene="rplL"
                     /locus_tag="PK733_12080"
     CDS             complement(6507..6890)
                     /gene="rplL"
                     /locus_tag="PK733_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ11314.1"
                     /translation="MASEKITNLIEEVKNLTVLELSELVKALEEEFGVSAAAPVAVAA
                     APAAGAAAPVEEEKTEFNVVLKEVGSEKIKVIKVVREVTGLGLKEAKDLVDGAPNTVK
                     ENVSKEEAASIEAKFKEVGATIEIK"
     gene            complement(6981..7514)
                     /gene="rplJ"
                     /locus_tag="PK733_12085"
     CDS             complement(6981..7514)
                     /gene="rplJ"
                     /locus_tag="PK733_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ11315.1"
                     /translation="MPSEKILDQKKEVVSDIAGKISSAKGVVLADYRGLTVEQDTDLR
                     RALRKAGINYHVVKNTLTRIAADELGLNQLDPFLKGPTAMAYSSEDPVAPAKIMNEYA
                     GKYDKLQLKAGVVEGKVIDAEGVKSLANLPSREVLIAQVLGGLNAPITGFVNVLNANL
                     RGLVVALNAIAEKRANA"
     misc_feature    complement(7546..7690)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(7736..8422)
                     /gene="rplA"
                     /locus_tag="PK733_12090"
     CDS             complement(7736..8422)
                     /gene="rplA"
                     /locus_tag="PK733_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ11316.1"
                     /translation="MKRGKRYLELAKLVDRETLYEPESAFELVQKTGNAKFDETVEAH
                     IRLGVDSRYADQQVRGAVVLPHGTGKTVRVLVFAKGDKAKEAEDAGADFVGAEDIIAK
                     IQNENWFDYDVVVATPDMMGVVGKIGRILGPKGLMPNPKAGTVTMDVAKAIKDIKAGK
                     IEYRLDKTNIIHCPIGKVSFGTEKLLDNYRTLLDAIIRAKPAAAKGQYLKSIVISSTM
                     GPGIKVNSAN"
     gene            complement(8539..8964)
                     /gene="rplK"
                     /locus_tag="PK733_12095"
     CDS             complement(8539..8964)
                     /gene="rplK"
                     /locus_tag="PK733_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOJ11317.1"
                     /translation="MAKKITGYVKLQIPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTAKDAGLIIPVVITIYADRSFTFITKTPPASVLLKKACKIESGSGTPNKEKVAKISK
                     AEVRKIAELKMPDLNAATVEAAERMVAGTARSMGIVVED"
     gene            complement(9104..9637)
                     /gene="nusG"
                     /locus_tag="PK733_12100"
     CDS             complement(9104..9637)
                     /gene="nusG"
                     /locus_tag="PK733_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOJ11318.1"
                     /translation="MLPEEVSEGLKWYVVHTYSGYENKVKANLEKIVENRGMQEYIVD
                     VVVPMEEQIEIKEGKKKTTLKKVFPGYVLIKMIMTDETWYIVRNIRGVTGFVGPLSRP
                     VPLTDDEVKVMGVEDFPPVVDYEVGDSVKVISGPLENFIGTVEQISLDKKKVRVSISM
                     FGRETPVELELIQIQKL"
     gene            complement(9709..9930)
                     /gene="secE"
                     /locus_tag="PK733_12105"
     CDS             complement(9709..9930)
                     /gene="secE"
                     /locus_tag="PK733_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOJ11319.1"
                     /translation="MAEKIKETKGKQMWKKTVRFFKEVRTELRKVVWLTRKQLANSLI
                     AILAICLLFGIVIWVADIGLSKIVDITLK"
     gene            complement(10002..10151)
                     /gene="rpmG"
                     /locus_tag="PK733_12110"
     CDS             complement(10002..10151)
                     /gene="rpmG"
                     /locus_tag="PK733_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOJ11320.1"
                     /translation="MRVKITMACSECKRRNYNTMKNKKNDPDRLEMNKYCKFCRKHTA
                     HKETR"
     gene            complement(10356..11336)
                     /locus_tag="PK733_12115"
     CDS             complement(10356..11336)
                     /locus_tag="PK733_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11321.1"
                     /translation="MKKNKFLELLVSKKNSLKETAYNENTVSSEAFNKKSTNDKVHAE
                     KIKKAYEILGVREGAGRDEIEFKYYLLAKKHLILKRENIKSEVVGLNMEKINEAYYCL
                     IGIDREKSQDASGSHEKQRNTLLYMLGDHKTAVLICLVILVAAIAVFAWEPVENNINI
                     VIFGEFMDDLVDINSLKDEIRNKLCNTIKHENLIIEEFMLSKELPIPYKIQMMSSAYN
                     FVTQGDYHILIMDKKSFNEYASVANIAKLDSLAEELKLDNEGCYLLKKQFGDHEEHIY
                     GLHTYCNGNSGEKNEEDHLSESSYIVVIKDGLDTKKSKTVTETIKLLVKH"
     gene            complement(11377..>11729)
                     /locus_tag="PK733_12120"
     CDS             complement(11377..>11729)
                     /locus_tag="PK733_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOJ11322.1"
                     /translation="SLGYYPFPTRKYGDIVLPAGNYQALKVVIGSGKGANWWCVLFPP
                     LCIVDVTHGTIPDSVKNELKDALSSDEYKIITSADNDKDIPVKIRFKIVEIFQNSRMK
                     FSGAISRIFNISEK"
BASE COUNT         3268 a         2585 c         1683 g         4193 t
ORIGIN      
        1 ttgctccatc aaaaacagga gttgcgattt tccatcccaa tgcttttgca gcataaccca
       61 gatgaacctc caacacctga cctacattca ttcttgaagg cactccaaga gggttgagaa
      121 ctatttccaa tggagttcca tcaggcagga aaggcatatc ctcttccggc aatatccttg
      181 atatgacacc tttatttcca tgtctgcctg ccattttatc ccctacggat atttttcttt
      241 tctgagccac atataccata accatctggt ttacaccagg cgggagttca tctccatttt
      301 ctctagaaaa gacctttaca tctacaatta ttccctgctc accatggggt acacgcaaag
      361 aagtatccct tacttctctt gctttttcac caaatattgc tcttaaaagt ctttcctctg
      421 ctgtaagctc tgtttctccc ttgggcgtaa ccttgcccac caggatatca cctgccctga
      481 cttcagcgcc tatccttatt attcctctat catcaaggtc ctttagcgaa tcttcactaa
      541 cattgggaat atctcttgtt atttcctcag gcccgagttt tgtgtctctg gcttccgctt
      601 catactcttc tatgtgcatg gatgtaaatg tatcatcctt aaccagcttt tcacttatta
      661 gaatagcgtc ttcataatta taaccttccc atgtcatgaa acctacaaga acgttcttgc
      721 caagagccaa ttctccattg tctgttgaag gtccgtcagc aataacatca cctgctttta
      781 ccacatctcc ctttctgaca ataggtcttt ggttgataca tgtgccctgg tttgaacgca
      841 tatatttaag aagcttataa gaatctctac ggttatcagc acgttttata gttatttcct
      901 tagctgttac cttttcaaca gtaccaccgt ttttagccag aaccacaacg cctgaatctc
      961 tggctgattt atattcaata ccagtcccaa ctattggtga ttgggttttt attaagggaa
     1021 ctgcctgacg ctgcatgttt gaacccatta gtgcacggct ggcgtcatcg ttctcaagaa
     1081 aaggaatcag ggcagttgca actgatacta tctgtttagg tgatacgtct atatagtcaa
     1141 ccctggattt ttcaacctgg aatatatcat ttttaaatct tacagtcacc ctatcgttta
     1201 caaaacgccc gtctttatca agcggttcat tagcctgagc tacaatatat tcatcttcta
     1261 tatctgcaga aagatagtca atttcatcgg ttacagtacc ggtctctttg ttgatccttc
     1321 tatatggggt ttctataaag ccataattat ttacccttgc aaatgtacta agggaaccaa
     1381 tcagtccaat attcggacct tcaggtgttt caataggaca tatacggcca tagtgagagt
     1441 ggtggacgtc tctgacttca aatcctgccc gttctctgct caatccgcct ggtcctaacg
     1501 cgcttaatct ccttttatgt gttagttctg caagagcatt ggtctggtcc ataaactggg
     1561 aaagctggct gctgccgaaa aactctttta ttgctgcagc taccggcctt atatttatta
     1621 gtgactgtgg agttactata tccatatctt gaattgtcat tctttctctt acaactctct
     1681 ccatcctggc aagacctatt ctgaattggt tttgaaggag ttccccaact gaacgaagcc
     1741 ttctgttccc aagatgatct atatcatcca catcgcctac accataacca agattaacaa
     1801 ggtaatttat tgatgccata atatcatcag atgtaatgtt tttgggcagc aggttgtcaa
     1861 tatttgcttt tattgccttc tttatatctt caggagtgtt gttttctgca agtatttttc
     1921 ccaattcagc aaggtatacg ttttcttcaa ttccaatatc ggacaaatcc agatctagat
     1981 attgtcttat atctacagta tgattaccta taactcttat cctttttcca ttcacattca
     2041 ggtaaacaga attgataccc gcattctgaa tttttgcagc agttgctcta tcgattactt
     2101 catctgcaga aataataatt tctccagtgc gtgtatcaat aacattatct gcagtaacaa
     2161 aacctgttat tcttgaagcc agtgaaagtt ttttattaaa tttaaatctt ccaaacttgg
     2221 caagatcgta tcttttaggc tcaaaaaaga gattgttaat aagctgtctg gcactttcta
     2281 cagtaggcgg ttcacctggc ctcaatcttt tatatatttc cagcaagcct tcctcttcag
     2341 tctttgagtt atctttctgt attgtagcaa gtaccctttc atcttcaccg agatattcca
     2401 taacgtcaag gtccatgcca taacccatgg cccttaccaa tacagttata ggcagtttcc
     2461 ttgttctgtc tattcttaca tatataatat catttgagtc tgtttcatac tcaagccatg
     2521 ctcccctgtt gggtataatt gtagctgagt atagtctttt gccagtttta tcaattttga
     2581 catcatagta ggtacctggt gatcttacca gctggcttac tataactctt tctgcaccat
     2641 taattataaa tgtaccggtt tctgtcatta aagggaaatc tcccataaat atttcctgtt
     2701 ctttaacttc tccggtctcc ttattgatca agcgcgcttt tacctttaac ggggcagcat
     2761 aagtagtatc tctttcctta cattcctcaa tagaatactt cgggttctca tccagggaat
     2821 acccgacaaa ctccattatg agattaccag tgtaatctgt aatcggagat acatccctta
     2881 agacctccat aagaccttca tcaaggaaat ttttgtatga atttttttga atttcaataa
     2941 gatcaggcat ctctaatact tcatcgattc ttgaatagct cattcttgta tttcttccca
     3001 tatatacggg atgcaccatt aaaaatcacc tcataaactt tatgttataa agacatagta
     3061 tacaaatcaa cgaatttata cgttttaggt atattttata tgtaattaca tataattttc
     3121 atatattatc tatttttctc atttatgcca ttaaattatt attaacagtt attgttttat
     3181 ttatacttcc atgctataat cataaacata gaatgaaaat atattagtat tataattttt
     3241 caaaaaaata acaagttata ttgttatata acttttttta aaaacgcaaa attacacact
     3301 tccccttata ttacaatgca gaataagatt ttaacatagt gaatgtaact tgtcaacaac
     3361 tttttgcagc ttttttcgat ttttaaggat attactccag taaataagta aacaaaaagt
     3421 gcaatatttc cctatttccc aggaaatatt gcaccaattc agtctagttc aagctttgtt
     3481 cagttaagcc atctttttta ccctattata tacatcctct ttaaatcttt ctataaaccc
     3541 ctctacataa cttattatat caagactttc atcctgaata agtggtgtaa gatccatagc
     3601 atatgttaac gctgtagtat tgtctgttcc atgtgattca gcatatgttg cattggcaag
     3661 tcttctacct gctgttgcca agtcatatct ttttcctccg cagatctgtg aatcatgtac
     3721 ttcaagtatt gcggctgaga tattttgatg tgatgctaca tcaaataata ctttttcatt
     3781 atcatttatc caatcaagac atctccagac ttcacatcca aataattttt caggacgttg
     3841 ttccgcaggg agccttctta ttgcctttat aactttaact gatgttccta cgtgagtatc
     3901 atgcttgtcc gctagattat gagtataaac aacctttggc tttgcgtgtg ctatcagctc
     3961 agcaagctcg ttaattacat cctcattatt agggtccttt acggccttac ttggataatt
     4021 cattagagca agtgcaccat attcacccac atatgctgct tttttctgtt ctttttttct
     4081 tacctgtttc atatcttcat cactataatt ggcatatagg tcatccctgg gacttcctgc
     4141 tccatcggtt aaaatagtgg caaaaaacca ttggtcttct ttcccaaaac actccataac
     4201 accatgatat gccattatct caatatcatc ctgatgggcc gagataccca tatgtgtagt
     4261 ccttgctata ccctcttcta ctgtaagatt atcgggtata aataattcag cacccggctg
     4321 cttcaatttc atattaagct ccattcctgc tgcgtgcgaa atgaattcgc ccttacagga
     4381 acaaaacttt ttataaaaaa gtttatatgg gcttaaattc atttcagtcg cgcgtgcaat
     4441 ttattttttc taaaattctt gttcaaaaaa tatgcacatg cgcattagtt tttcagaaat
     4501 taacgatctc tttcaaacta aactccattc ctgctgcgtg tgaaatgaat tcacccttgt
     4561 aggaacaaga cttttaataa aaaagtttat atgggcttaa attcatttca atcgcgcgtg
     4621 caatttgttt tttaaaaatt cttgtgcaaa aaatatgcac atgcacatta gtttttcaga
     4681 aatttaacaa tttctttcaa actatcattc tataataaaa catttatctg atttccggca
     4741 agcttgcagc agcaattgct tgtccaactc ttcttgttga ttcgtcaggc agatgcaatt
     4801 ttacttttgc agcaagatcc gggaattctc ctttcagtac ttcttcggct ttatgcagta
     4861 ttaaatttcc tccttcgcct gaagtaacac gtccaagtat aagtatatgc ttaatatcat
     4921 aaaattctgc atagtaagca agagaataac ctaaataaca gcctatgctt tcaaatatac
     4981 cttttgcgcc ctcatgaccc tcatgaagca atttttgaac aactttcaat ttctccgcag
     5041 gggttaaatt acctgccagt tctattcctg ctgcaggagc aagcttaata acagcatctt
     5101 gcgaaaaata tttaactcca catccaaaat ctcccgacca ttcgtccacc atggattcag
     5161 tattgaaatc tacaggaaca aacgcaagtt cattgagcca acctgttaaa ttgccatgac
     5221 agtctacata tccgcctgct tcgcttgtgc ccatggctat tccaagtaca ttggtgtcat
     5281 tcagctccat agccccggcc aatgcagtaa catctccatc atttgcaatc tctatgggta
     5341 taccgcccat ttctttttga atatcgagga atatgttctt tactttttta tcaaaggcat
     5401 catcaggaac tttaataaat aaagatgcaa ccataaccct gttatttata tatatacctg
     5461 cagcacttac accaatagca tctacatggg gcaagtagga tgctgcagtt tttattgcct
     5521 taagtatctc actataatgg taatcagggt tgctctctgt ttttggatgc caaataactt
     5581 cttcgctgta aacagcgttt ccatcaataa ctgctgctac tttacggtcg ctccctcctg
     5641 catcaaaccc gatcctgttt ccattaaaat tgcgccctat tggctgagtt tcttcttttg
     5701 cacagggtac attatcaagg tcggttattt ctacagtaaa gtctttctca taaacccttc
     5761 ccataaattt ggcatcaaat tctctaagcc ctccctggga ataagcccta ctaatatatt
     5821 ctccgatgct gacaggtcct ccgattatga ttttccaacc gccacgcatc catagaagag
     5881 tttttaaaag acgttcaaca taaaaaaagt tttcctcatc cattccggta ccttccctga
     5941 atacttcagt atggtacgta gatattaaac catcatttcg ctcaagtgca atatgtaaag
     6001 gtacaccttt cccggattct tttacagctt ttataaattc acggtttgct agtattgcag
     6061 gttcaaaatt tttgtccaat acaggggtaa ataatggatt gatgataatt tttccggact
     6121 tcatggtagc cctccctctc tttttcaaat tgttaatttt agaaatatca attgtgcaat
     6181 tatgaaataa atattatgtc gaatatatcc tctaactatt atttatattt taaattaggt
     6241 ttgttaagaa gtcaatatat ggagatgtga agggaggcat tttcttccta tgatacgaaa
     6301 tatggcagca tacgattttc tgagtataaa atttgaacaa agcttacgga tttttctgta
     6361 gacttaaact ctacaatctg ttgctgaagt gaatattata catggaaatc gtttcttgcc
     6421 atatttttct tcaaatatag aaaatgtgcc ctgtaaaaaa ggaggtctta atgacctcct
     6481 ttttttgtcc gtaccttatc agtactttat ttaatttcga ttgttgcgcc aacttcttta
     6541 aatttggctt caattgaagc agcttcttcc ttggaaacat tttctttaac agtgtttggt
     6601 gctccgtcta caagatcttt tgcttcctta aggccaaggc ctgtaacttc tcttactacc
     6661 tttataacct ttattttctc agatccaact tcctttaata ctacgttaaa ttcagtcttt
     6721 tcttcttcaa ctggagctgc tgctccagct gctggtgctg ctgcaactgc tacaggtgct
     6781 gctgcggata ctccaaattc ttcttccagg gcctttacca gctctgataa ctctaatact
     6841 gttaagttct taacttcttc aattaaattt gttatttttt cacttgccat tatcatatac
     6901 ctccataaat tttcaatttt taatttttgt atatttttct ttttcttata aaacataaat
     6961 caagttatat tttacatctt ctatgcgttt gctctttttt ctgcaattgc atttagtgcc
     7021 actactagac ctcttaagtt tgcattcaat acatttacaa atccagttat tggagcattt
     7081 aatccaccaa gtacctgtgc aataagcact tctcttgatg gaaggtttgc cagggatttt
     7141 acgccttcgg catcaattac cttaccttct actacaccgg cttttaattg cagcttatca
     7201 tacttcccgg catactcatt cattatttta gcaggtgcaa caggatcttc actgctgtaa
     7261 gccatggctg taggtccttt aaggaatgga tcaagttggt ttaatcccag ttcatcggct
     7321 gcaatccttg ttaaggtatt ctttactaca tggtaattta taccagcttt tcgcaaggcc
     7381 cttctcaagt ctgtatcctg ttctactgta agtcctctat agtctgcaag aaccacccct
     7441 tttgctgatg aaatcttacc tgctatgtca cttacaactt cctttttttg atcaagtatt
     7501 ttctcacttg gcattgttgt acattcacct cctctgaaac tcggcattaa aataaattaa
     7561 cccttcattt gcacatagac acatgaaggg ttattgacct tttcaaatca actttcctta
     7621 cctcggcagg aagataatat atcaattatg ctttcgcacc tgctgtctat ggcaaagcgg
     7681 ctattcactt ttaagtgtta ctattttaca tcttcacatc tttatttata ccaggttagt
     7741 ttgctgaatt aactttaata cccggtccca ttgttgatga tataacaatg gattttaaat
     7801 attgtccctt ggcagctgca ggttttgctc taattatagc atccaataat gttctatagt
     7861 tatccaaaag cttttcagtt ccaaatgaaa ctttaccaat tggacagtgg atgatatttg
     7921 ttttatcaag tctgtactca attttaccag ctttaatatc tttaatagcc tttgctacat
     7981 ccattgttac agtacctgct ttagggttag gcataaggcc ctttggtccc aatattctac
     8041 caattttacc tactactccc atcatgtcag gtgttgctac aactacatca taatcgaacc
     8101 agttttcatt ctgaatttta gctataatat cttcggcacc tacaaaatcg gcacctgcat
     8161 cttcagcttc cttagcctta tcaccttttg caaagactag aactcttaca gtttttcctg
     8221 tcccatgtgg aagtactact gcgcccctta cctgctgatc agcatatctt gagtcaactc
     8281 caagtcttat atgtgcttca acagtctcat caaactttgc attacctgtt ttctgtacca
     8341 gttcaaatgc actctcaggc tcgtaaagag tttctctatc tacaagtttg gctaattcca
     8401 aatacctttt tcctcttttc atattccttt cacctccgtg gttagttttc gggaaaattt
     8461 tccctcccac tcaactaaat taacgacgac tgtactaaaa taaacgcatg cgtcatacaa
     8521 atctgttact actattaact aatcctctac tacaataccc atacttcttg ctgtgcctgc
     8581 taccattctt tcagcagctt caacagttgc tgcatttagg tcgggcatct ttaactcagc
     8641 tatttttcta acttctgctt tggatatttt agcaaccttt tctttattcg gcgtacctga
     8701 accgctttca attttgcatg cctttttcag taatacagaa gcaggcggag tttttgtaat
     8761 aaatgtaaac gatctgtctg catatattgt aattacaaca ggaattatca gaccggcatc
     8821 ttttgcagtc ctttcattaa actccttaca aaatcccatt atgttaacac catgttgtcc
     8881 taaagctggt ccaaccggtg gagccggagt tgccttcccc gcaggtatct gtagctttac
     8941 atagcctgtt atcttttttg ccatattttt tagtccacct cccaatttgt ttaaaaataa
     9001 attatagcat ataatatcaa taactttaag ccataatctt taaatcttta aaattttcga
     9061 ctgctttccc aatattaaat attaaaaaac tacgaacttt aaattatagc ttctgaattt
     9121 gaatcaattc caattcaacg ggtgtctctc ttccaaacat agatattgaa acacgcacct
     9181 ttttcttatc caggctgatt tgctcaactg tcccaataaa attctcaaga ggtccggata
     9241 taacctttac actatcccct acctcgtaat caacaaccgg cggaaaatct tcaacaccca
     9301 taacctttac ttcatcatca gttaaaggca caggacgtga taaaggtcct acaaacccgg
     9361 tgacacctct tatattcctt acaatgtacc atgtttcatc tgtcattatc atcttaataa
     9421 gaacatatcc ggggaaaacc tttttcaatg ttgtcttctt tttaccttct ttaatttcaa
     9481 tttgttcttc catagggacg actacatcca cgatatattc ctgcataccc ctattttcaa
     9541 caattttttc aaggtttgcc ttaaccttgt tttcataacc agaataggta tgtactacat
     9601 accatttcaa gccttcagac acttcttcag ggagcattaa actttatacc ctccttgctt
     9661 tttttgggca catacaacca gactgactta agctattacc ttaaaaactt attttaaagt
     9721 aatatctact atttttgata gtccaatatc tgctacccat ataacaattc caaatagcaa
     9781 gcatatggca agtatggcaa tcaaactatt agcaagctgt tttctggtaa gccatactac
     9841 ttttcttaac tctgtcctga cctctttaaa gaatctaaca gtctttttcc acatttgttt
     9901 gccttttgtt tcttttattt tttcggccat agcaatacaa ctccatcttt taattttgct
     9961 gcttctttta tcaattaaat tatgactata tcacaaaaat attaccttgt ctctttatga
    10021 gccgtatgtt tacgacagaa tttacaatat ttattcattt cgagcctgtc aggatcattc
    10081 ttcttattct tcattgtatt ataatttctg cgtttacatt cggaacaggc cattgttatt
    10141 ttcactctca acactaacac ctccagcttt gctaattggc aggtactttt cttttgacat
    10201 acctgtccat ccaatacatt gcacctaaat ggaatatatc attacataat aatgtttact
    10261 taataaaata ttgtcctggt ataggcacaa aaaaaaatcc tatagtagga gcaatattta
    10321 ctctaacata aaagtgtatg caagtcaaga tttttttaat gctttacaag caattttata
    10381 gtctctgtta cagttttgct tttttttgta tccaggccgt ctttgattac aactatatat
    10441 gaagattcac ttaaatgatc ttcctcattc ttttctccac tattaccatt gcaatatgta
    10501 tgtaagccgt aaatatgctc ttcatggtct ccaaattgtt ttttcaataa ataacatcct
    10561 tcattatcca acttcaactc ttctgcaagt gaatccaatt ttgcaatatt tgccacagag
    10621 gcatactcat taaagctttt cttgtccatt atcaagatgt ggtaatcacc ttgcgtaaca
    10681 aaattatagg cagaagacat catttgaatt ttatacggaa tgggtaattc tttcgagagc
    10741 atgaactcct ctataattaa gttttcatgc tttatagtat tacagagttt atttctaatt
    10801 tcatctttta gtgaatttat atcaacaagg tcatccataa attccccaaa aataactata
    10861 ttaatattgt tttcaacagg ctcccaggca aatacggcaa tagcagctac taatattaca
    10921 aggcaaatta aaaccgcagt tttatgatct ccaagcatat acaacagagt gtttcgctgt
    10981 ttttcatgag aaccacttgc atcttgagat ttttccctat ctattcctat taagcagtag
    11041 taggcctcat taattttttc catattgagc cctactactt cacttttaat attctcacgc
    11101 tttaatatca ggtgtttttt agccagcagg tagtatttga attctatctc atccctacct
    11161 gccccctccc ggacacctaa tatttcgtaa gcttttttaa tcttctcagc atgtacttta
    11221 tcattggtgc tttttttatt aaaagcttca ctactaacag tattctcatt ataagccgtc
    11281 tctttaagac tatttttctt tgaaactaat aattctaaaa acttattttt tttcatttcg
    11341 ctttccaaat tggcatagta gtactaatta ccagtgttat ttctcggaaa tattaaaaat
    11401 tctgctaatt gcaccggaaa acttcatcct ggaattttgg aaaatctcaa ctattttaaa
    11461 tcttattttt accggtatat ccttatcgtt atcggcggat gtaatgattt tatactcatc
    11521 actgcttaaa gcatctttca attcgttctt tacagagtca gggatagtac catgagttac
    11581 atccacaata caaagaggag gaaatagcac acaccaccaa ttggctccct ttccgctgcc
    11641 aattacaacc tttaatgcct gataattacc agcaggtaaa acaatatccc catactttct
    11701 tgttgggaaa ggataatatc ccagagatg
//
LOCUS       DAOSDD010000136        11706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2117_length_11706_cov_5.194833, whole genome shotgun sequence.
ACCESSION   DAOSDD010000136 DAOSDD010000000
VERSION     DAOSDD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2117_length_11706_cov_5.194833"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK733_12125"
     CDS             <1..343
                     /locus_tag="PK733_12125"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11323.1"
                     /translation="NMVGNLGKPFYINTYNKGSITNMKNDAFLELCCQVTMDGIKPYS
                     VGEMPKGIRGMQEIVLDTHELTAEAIYECNYDKLRRAFLTDPLICSIVDADNIIKELL
                     QVEREALPECW"
     gene            334..471
                     /locus_tag="PK733_12130"
     CDS             334..471
                     /locus_tag="PK733_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11324.1"
                     /translation="MLVKERRVDMRLYHLDLKFAMYKKEYIKNWFEKLKANGFDGRVN
                     I"
     gene            1492..4725
                     /locus_tag="PK733_12135"
     CDS             1492..4725
                     /locus_tag="PK733_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ11325.1"
                     /translation="MSDKFRIKDIVPTVFLKREENELKQLIKLEINSSISREAMILVI
                     AKEKINEISLGIVPNGVSIQRIFIDEMKEKTDIKFVLKSQGIVYDSRTESWNPPRHWR
                     VHVVQLSHHDPGYTDLPSNVLKTHIRWLDEALLFAEETGNFPEDARFRMVIEQAWSID
                     HYLKNSPESKVNRIIDLMRLGRIELTALFGNMITEICGHEEMIRTLYHAFSLKRKYDI
                     PIVSAEHNDITGISWGLCRVLTDAGIKIFCPGIPLYYNWAVKDANGLSLNSFWDEKAI
                     FTHGGPGAFWWESPSGKKLLFWCNNQGCGGDYHAGLPGLPEKLIQLEEQDYPYHVIRW
                     PVSGGARDNSPYIGGYAYTIKEWNEKWEYPHLICSTNAKFYEDFIGEEPKDLPVFKGE
                     LPGQDYPVGSTSTAASTAQNRNNHSAMITAEKLASMAEIVTDYTYQKEDISQAYEDML
                     WYDEHAWGHHFPCGPAMKASQYEKMIRAYRASAFAHDITSKAMAKIADNIKFEEDGFY
                     LVVFNSTSHKTIRPVRTLLREIDNCGSTMYEVPPEEDPKGEGYFKGVLLGDRWHASPS
                     LDIVNGRFELMDMSTGKCVPFQIIEIQTAMDAVPYAGERLGIGSGTSRYGFFEVPLGL
                     KKDLCFIAEDIPPLGYKTYRLISKKDTKGIEYSESKRYSESKRYSESERYSESERYSE
                     SERIMKYNCCNSVEDFIAIENEYYRILVDKCTGNVKSILDKEVARDIIDNACPHGFNS
                     FIVREPDFGDKKRTEYYIENVKVSKRLDEDICKAIEISSGACGHPVIKQEIVLYKGIK
                     AIYFNIKVIKDPTPLLDVHIAFPFKVDNPVFRYEGVLSVINPIEDYFPGAYLDTVAVQ
                     NWVKVTDGSYSVLWSGLDAPIAGFSGLHPGYVSPAHRCLIDGKAAHPPMKKEELTKGW
                     IYSNIFYNNFGTNFSVSQTGEMLFRYVITTCSHDISDSEASAFGWQAVTPVEQIFTKR
                     YSKDVKTGKMPVEYSFIEYDNKNAAILAFKKAEDDNGYILRLWNMENKAVNIDLKLNG
                     LRIDSLYLADVVERNSSIITSQKENSINVSIDKNSIVTIRIIKKNDN"
     gene            4850..6637
                     /locus_tag="PK733_12140"
     CDS             4850..6637
                     /locus_tag="PK733_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11326.1"
                     /translation="MIKRKGISIRTKLIFIFLAIILFTSFCISIFSYKASENTIKMQS
                     EELISGYLNQSVKRFDLFMENLHRASMSIAFNKDLNKILARINQGSYEDYNNLLRIND
                     LISTFNNTNRFVYLVNIYDFKNGVIIPSRGTKRKINLEDSEYKLVKSLLIDNSDAYKI
                     LYHWVESRIINLNGVEKNVLTFAIPIKDNFTGEIIGSVMTYVPEEHISGMCLEISFSS
                     GGNAILVNSSGKAISSSEPNLVGENFFDYLNVERQGNDSYIRKIDGKEYLVSYRKSNY
                     IDWSFLSIVPIKIIMQKNTGVLRNTFVFIGLGTILLAFLLSYIVNFYFYKPIRYLIDD
                     IKAHIKEKNLDLKPISRDDEIGFIFNSFYEILLENRVLIENIYEQKILLQEAEIRLLY
                     SQINPHFLYNSLDSIIWMLMFKQYDDIGILVKALVKFFRITLSEGNEIITVRQAKERI
                     ESYFIIQKIRYSDRLIVDVDFEESILEFKMLKLMLQPVVENSIYHGVEKKLGEGIIKV
                     TGRQEGDVLVFKVEDNGVGISPQRLDEVNKIINSEEQQLDQFYALQNINKRIKIFYGD
                     NYGISLESSEGEGTIVNIKVHVIEVPVSE"
     gene            6660..7712
                     /locus_tag="PK733_12145"
     CDS             6660..7712
                     /locus_tag="PK733_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11327.1"
                     /translation="MKLLIVDDEPMVRKGLLYGFDWLSIGVEAAGTASNGIEAIEFIK
                     LNQPDVVLADIKMPGMNGLELGKWVKNNYPYIDVIFLTAYGDFDYARQAIELEAANYL
                     LKPVDADVLFSTMEKIKKKTYDEKKRKIVYKTDKEKLKSIIVNHNMEALNEYLHEYFN
                     LYRKHLNIKLDNKELIIFELISAVYEVIREHEKNGSELALDSVTLEEFAKLQTFEELQ
                     KEITRFLIECVDNALNKNNECYKHDIDKIIKYTRNCYMKDINLKTVSSTFSINPSYLS
                     RVFSESTGVSFTNYLNELRMLNAKELLKNTDFSVKTVSKRVGYNNEKYFIRIFKKVEG
                     ITPQEYRTSNKINKSN"
     gene            7984..8787
                     /locus_tag="PK733_12150"
     CDS             7984..8787
                     /locus_tag="PK733_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11328.1"
                     /translation="MAFKDYQVGRGFKGIFTSPSVGFEHFQRLFKSYYFFSILRNTIL
                     ISFYKIIFCFPAPIIFAILLNELRITYFKRFVQTVTYLPHFLSTVVVTGLVLAMLSPS
                     YGLVNAVLGTMGLEPVHFLANSRYFRSIIVAIDVWKGTGWSAVIYLAAISSIDPQLYE
                     SATIDGAGKFRQIWSITLPSLLDLIILLLILRIGDILNAGFEQIFLLYSPPVYSVGDI
                     IDTFVYREGLVNSNYGFSTAVGLFKSVVGLVLIVGSNKLANRFGKQGIW"
     gene            8821..9705
                     /locus_tag="PK733_12155"
     CDS             8821..9705
                     /locus_tag="PK733_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11329.1"
                     /translation="MVKQSKGEKIFNFINILIMLIAVIAVIIPLFSVVSTSLVSNQEL
                     SRRRFIIFPQKVDFTAYKIIFEGTSIIISGYKITIFRVVVGTVINLIFSYFTAYAMSK
                     KDLPGRNVITIFFFFTMLFGGGLIPYYIVIKSVRLLNNVWVYILPQIISVWNTLLIRN
                     FIMNIPESLEESAEIDGANAVHIIFRIILPLSIPALVTIGLFYAVGHWNSWWDAFLFV
                     SDVRKQPIQLVLRNILASAEMRINTITGRIDATEIKPPSRAIQTATIVVTTVPIVIIY
                     PFIQKYFIKGIMIGAVKG"
     gene            9785..11326
                     /locus_tag="PK733_12160"
     CDS             9785..11326
                     /locus_tag="PK733_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11330.1"
                     /translation="MLKKRLLTMLLCLVLIASLFTACGKSGTNAEKTDGSDTSPLKEN
                     KEETVTIVMERPLWQNADPSSDPNMRIRQKIKEKINIDVEVVGQQNPPDQAEKPNLMI
                     AAGETLDIFQTPFGGSNDWRKYKREDTIIPLNDLLNEFGVDLLKHVNQDALKACTDED
                     GKIWALPDEQNPVTTFLMMRKDWLDKYNLSVPETFEEYENVLQVFKEKENDEGIVPFW
                     QGLEDNIFAGCFIPTGDQKFLDSDGKLKPNFMHPNYKEFLAKMADWYKKGFLHKEFAT
                     MQYQQAADLAFGGRSGLFVNWISGSFKFNVDTLKNSVPDGELIVIAPPKGPAGRLIRK
                     GLPITSNIMITKSSKYPEKSIEFLNWSMATDEGWLLSRFGEEGYDWNWIDKSKNILDY
                     TDKKVDVDRYGYAIFCSTRLNKFVNKYNIGGMVDSEATAFVHDENKYPTKNPIDLLVI
                     YDVNKMKSKDKLNSMNTFLNEAKYQIIMGQEQVSSWDNVIKKWLELGGKEYIDDITEQ
                     FNAQK"
BASE COUNT         4148 a         1634 c         2330 g         3594 t
ORIGIN      
        1 aaacatggta ggtaaccttg gcaagccatt ttatataaat acatacaata agggttcaat
       61 taccaatatg aagaatgatg catttcttga gttatgctgc caggttacta tggatggaat
      121 aaagccatat tctgttggag aaatgccaaa gggtataaga ggaatgcagg agatagtact
      181 ggatacccat gagcttacag cagaggctat ttatgaatgt aattatgata aattaaggag
      241 ggcatttcta actgatccat taatatgttc tatagttgat gctgataata ttattaagga
      301 attgcttcag gtggaaagag aggcactgcc tgagtgctgg taaaagaaag aagggttgat
      361 atgcgtttat atcacttgga tttaaagttt gcaatgtata aaaaagaata cattaaaaat
      421 tggtttgaga aactgaaagc caatggattt gatggtaggg taaatattta ataggtgcat
      481 ttatcaaatc cgaggaaata tttaccagga gctatcgtta cggtgcttag aacctttaaa
      541 ttcagctatg ggcaaaatca aactcacaaa ataatcttat caaaatagtt gtaattattt
      601 tgttcaaaca ctgattttgc ttatccttcg ctcagatacg gggaaactct tcaacaaaaa
      661 agggaggtct cttacttgag tgaagaggtt cccctgccca gctacagcac cttcactaat
      721 gccccttacc aatattttct tcagataaat taaatgcatc gatgtaactt acaatttgct
      781 aattcttcat tgattatttc aaatcgtaaa ttatatagtt taatctgaaa aaataaatga
      841 cagtaatttg gaaattgaca catagggaaa agaaaaattg aaatattgaa aaagaaagga
      901 aagtagtata cttaatcatt gtaggaactg tgctcaatat ccaatatcaa tatccaatat
      961 cactcaaaat aaatatccat atgagcatga aattggggat tgtatgacta aatgcattct
     1021 ggtgtttgta agacttattg caattaattt cattcgttaa agtcgaattt actttgtcaa
     1081 ctgaggaaaa ttatttaaag gaatgagaaa gcattttttc ttattgacat gataatgcta
     1141 atagtataca ataagctaaa ccggttttgt tttacactgt tttaatatta atgctatcat
     1201 gtggcgctga gaacccgatc tggagataag attataatac cgaattacat agactttatc
     1261 taaatatact agctgaaaag cgttgttctt ttcaactgat gcaatgataa atatataatt
     1321 aaggtatcat gagaattgtg atatacaaag ttacagttga aaagatttat tgtgaaaagg
     1381 gaaatagtaa atatagaagc attgatatga aagtatgaaa gatgtgtatg tgaatcatca
     1441 catagatttg agataaatga aaaccattat aaaaccgaga ggaatgattc catgagtgat
     1501 aagtttcgaa taaaagatat tgtacctacc gtttttttaa agagagaaga aaatgagtta
     1561 aaacaactta taaagcttga aataaattct tcgatttcca gagaagcaat gattctggtt
     1621 atagccaaag aaaaaattaa tgaaatatct ttgggaattg ttccaaacgg tgtatccatt
     1681 cagagaattt ttattgatga gatgaaggaa aagacagata ttaagtttgt tttaaaatcg
     1741 cagggtatag tctatgacag cagaacagaa tcctggaatc cccccagaca ttggagagta
     1801 catgttgtcc agctttcaca tcacgacccc ggctataccg accttccatc aaatgtattg
     1861 aaaacacata tccggtggtt ggatgaagct ttgttatttg ctgaggagac tggtaatttc
     1921 ccggaggatg caaggtttag aatggttatt gagcaggctt ggtctattga tcattatctt
     1981 aaaaattcac ctgaatcgaa agttaacaga ataattgatt taatgcgttt aggacggata
     2041 gagctgactg ccctttttgg caatatgatt acggaaatct gcggtcacga agaaatgata
     2101 aggactcttt accatgcttt ttcgctaaaa agaaaatatg atattccaat tgtatctgct
     2161 gaacataatg acattacagg aataagctgg ggtttatgta gggtgcttac tgatgcaggg
     2221 ataaagatat tctgtccggg gattcctctt tactacaact gggccgtaaa agatgcaaat
     2281 ggactaagtc ttaattcgtt ctgggatgaa aaggcaatat tcacacacgg cggtcctggt
     2341 gctttttggt gggaatcacc ttcgggtaaa aagcttctat tctggtgtaa taaccaaggg
     2401 tgcgggggag attaccatgc aggacttccg ggactaccgg aaaaactgat acagcttgaa
     2461 gaacaggatt atccatatca tgtaatacga tggccggtaa gtggtggggc aagagataac
     2521 tctccatata taggtggata tgcatataca ataaaggaat ggaatgaaaa atgggaatat
     2581 ccacacctaa tatgcagcac caatgcaaaa ttctatgaag acttcatagg agaagagcct
     2641 aaagatctac cagtatttaa aggagagcta cctggacagg attatccggt aggttccact
     2701 tcaacggctg cttcaactgc ccagaacaga aataatcatt cagcaatgat tacagcagag
     2761 aagcttgcct ctatggcaga aatagtgacc gactatacat atcagaaaga agatatttca
     2821 caggcttatg aggatatgct ctggtacgat gaacatgcat ggggtcatca ttttccgtgt
     2881 ggacctgcca tgaaagcatc acagtatgaa aaaatgatac gtgcctatag agcttcagct
     2941 tttgcccatg atattaccag taaggctatg gcaaaaattg cagataatat aaaatttgaa
     3001 gaggatggtt tttatctggt ggtatttaac tcaacctcac acaaaactat aagacctgtc
     3061 cgcacacttt taagggagat agacaactgc ggtagtacaa tgtatgaagt acctcctgaa
     3121 gaagatccga aaggtgaagg gtattttaaa ggagtgctct taggagacag atggcatgca
     3181 agtccatcgc tagatattgt taatggtaga tttgagctta tggacatgtc aacaggcaaa
     3241 tgtgtgccat ttcagataat tgaaattcaa acagccatgg atgctgtacc ttatgcagga
     3301 gaaagactgg ggattggatc aggtacaagt agatatgggt tttttgaagt accattaggc
     3361 ttaaaaaaag acctttgctt tatagcagaa gatattcctc cgttaggata taaaacgtac
     3421 agattgatat ccaaaaagga taccaaagga attgagtaca gtgaatctaa aaggtacagt
     3481 gaatctaaaa ggtacagtga atctgaaagg tacagtgaat ctgaaaggta cagtgaatcc
     3541 gaaaggataa tgaagtacaa ctgttgtaac tcagtggaag attttatagc aattgaaaat
     3601 gagtattaca ggatattagt tgataaatgt actggtaacg taaagagtat actggacaag
     3661 gaagtagcac gtgatataat cgataatgcg tgtccacacg gctttaactc atttattgtt
     3721 cgtgaaccgg attttggtga caagaagaga actgaatact atatagaaaa tgttaaagtc
     3781 agtaaaaggt tggatgaaga tatttgcaaa gctattgaaa tttcaagtgg tgcttgtggg
     3841 catcctgtga taaaacagga gatagtcctt tataagggga tcaaagcaat atatttcaat
     3901 ataaaagtaa tcaaggatcc aactccattg cttgatgtac acatagcatt tccatttaag
     3961 gttgataatc ctgtgtttcg gtatgaaggt gtattgtctg taataaaccc gattgaggat
     4021 tattttcctg gtgcctactt ggacactgtt gctgtacaga actgggtaaa ggttacagat
     4081 ggcagctaca gtgtgctatg gtctggcctt gatgctccta ttgcaggttt ttcagggctt
     4141 catcccggat atgtttcccc agctcacaga tgtcttattg atggaaaagc agcacatcca
     4201 ccaatgaaaa aggaagaact tacaaagggg tggatatatt caaacatatt ctataacaat
     4261 tttggcacta acttctctgt atctcagacc ggagaaatgc ttttcagata tgtgataacg
     4321 acatgcagtc atgacatttc agatagtgaa gcatctgctt ttggctggca ggcggtaaca
     4381 cctgttgagc aaatatttac aaagaggtat agtaaggacg ttaaaactgg aaagatgcct
     4441 gtagaataca gctttattga gtatgacaac aaaaatgctg caattcttgc ctttaaaaaa
     4501 gctgaggatg acaatggata tatactgcga ttatggaata tggaaaataa agcagtaaat
     4561 atagatctaa aactaaatgg tttaaggatt gatagtttgt acctggctga tgtggttgaa
     4621 agaaatagca gtattattac gagtcaaaaa gaaaactcta ttaatgtaag cattgataaa
     4681 aatagtatcg taaccataag aattatcaaa aagaatgata attaagtgtt tgagacatac
     4741 tgctataaat taaaagcaac cttccgaaag ataatacttg acttgatttt ggaaatgaag
     4801 taaaatttat ataaaccggt ttatataact gtaaaagggg gattccttca tgattaagcg
     4861 taaaggaatt tctatacgaa caaaattaat attcatcttt cttgcaataa ttctatttac
     4921 ttcattttgt atctccatat tttcatacaa agcttctgaa aatactataa aaatgcaatc
     4981 tgaggagttg atatcaggtt atcttaacca gtcggtaaaa aggtttgact tgtttatgga
     5041 gaacctgcat agagcgtcca tgtccattgc atttaacaag gacttaaaca aaatattggc
     5101 aagaataaac cagggaagtt atgaggatta taataatctt ctaaggataa atgacctgat
     5161 atcgactttt aacaatacaa acagatttgt ttacctggtt aacatatatg attttaaaaa
     5221 cggtgtaata attccttcaa gaggtacaaa aaggaaaata aatctggaag attcagaata
     5281 caagcttgtc aaaagtttgc ttattgataa cagtgatgca tataaaatac tgtaccattg
     5341 ggttgaaagt aggattataa atttaaatgg tgtagaaaaa aatgttttaa cttttgctat
     5401 cccaataaag gacaatttta ccggtgaaat cataggcagc gtaatgacat atgtcccaga
     5461 agaacatata tccggtatgt gtctggagat atccttttct tctggaggaa atgccatact
     5521 agtgaacagt agcggaaaag caatatcatc ttctgagcct aatctggttg gcgaaaattt
     5581 tttcgattat ttaaatgtgg aaaggcaggg aaatgattcg tatatcagaa aaatcgacgg
     5641 aaaagagtat cttgtttcat acagaaaatc aaattatata gattggagct ttctttctat
     5701 agttcctata aagataatca tgcagaaaaa tactggtgtt ttgaggaata cttttgtttt
     5761 tattggcttg gggacaattt tactagcatt tcttctttct tatatagtaa acttctattt
     5821 ttataaacca ataagatatt taatagatga tattaaagct catatcaaag aaaaaaacct
     5881 tgacttaaaa ccgatttcaa gggatgatga aataggtttc atttttaata gcttctacga
     5941 aatattatta gagaacaggg tacttataga gaatatatat gagcaaaaga ttttgcttca
     6001 agaagcagaa atcaggctgc tttattcaca gataaatcca catttcttat ataattcact
     6061 tgattctatc atatggatgc ttatgtttaa acagtatgat gacataggaa tccttgtaaa
     6121 agctctggtt aagtttttta gaattaccct tagcgaaggt aacgaaataa ttactgtaag
     6181 gcaagcaaaa gaacggatag aaagttattt tataatacaa aaaataaggt attcagacag
     6241 gcttatagtt gatgtggatt ttgaggaatc aatacttgag tttaaaatgc taaaacttat
     6301 gctacagcca gttgtagaga attccatata tcacggtgtt gagaaaaagt taggtgaagg
     6361 cattataaaa gtaacaggta ggcaagaggg tgatgtctta gtatttaaag tagaggacaa
     6421 tggtgtagga atatctcccc aaaggcttga tgaagtgaat aagattataa attcggaaga
     6481 gcagcaatta gatcaattct atgcactgca aaatataaat aaaaggataa agatctttta
     6541 tggcgataat tatggaatat ctcttgaaag cagcgagggt gaaggtacaa ttgtaaacat
     6601 taaagtgcat gtaattgaag tacccgtaag tgaataaaca ttaggaggat ctggatataa
     6661 tgaaactttt aatagttgat gacgaaccga tggtcagaaa agggctttta tatggtttcg
     6721 attggttaag tataggtgta gaagcagcag gaactgcatc caatggaatt gaagctattg
     6781 aatttataaa gcttaatcag cctgatgtag ttttagctga tattaaaatg ccaggaatga
     6841 atggtttgga acttggtaaa tgggtaaaga ataattatcc atacattgat gttatcttct
     6901 taacggcata tggtgacttt gattatgcaa gacaagctat tgagctagaa gctgctaact
     6961 atcttttaaa acctgttgat gctgatgtat tgtttagtac tatggaaaag atcaaaaaaa
     7021 agacttatga cgaaaagaaa agaaagatag tttataaaac tgataaggaa aagcttaaat
     7081 ctatcatagt aaaccataat atggaggcat tgaacgaata tttgcatgaa tactttaact
     7141 tatataggaa gcatctcaat attaaattgg ataataaaga gcttataatt ttcgagctta
     7201 tatcggcagt atatgaagtg ataagagagc atgagaaaaa tggcagtgag ctagcacttg
     7261 atagcgttac attagaggaa tttgcaaaat tacaaacatt tgaggaactt caaaaagaaa
     7321 tcacaaggtt tttaattgaa tgtgtagata atgcgttaaa taaaaataat gaatgctaca
     7381 agcatgacat agataaaata attaaataca ccagaaattg ctacatgaag gatataaatt
     7441 taaagactgt atcatcaact ttctcaataa atccatcata tttaagcagg gtattttctg
     7501 aaagtacagg ggtatcattt accaactatc tcaacgaact aagaatgttg aatgcaaaag
     7561 aattactgaa aaatactgac tttagtgtta aaacagtaag caaacgggta ggatataata
     7621 atgaaaagta tttcatcagg atatttaaaa aagtggaagg gataacgcct caagaatata
     7681 gaacatcaaa taaaattaat aaaagtaatt aaagtgcact gtcaaagtaa tgatttgtgt
     7741 actttacaca atagaatttc cactatagaa ttttaatata gagtcatacc ataaaatatt
     7801 aatatgaaga acgaaaggtt aataataatg gaatctatac taaaatgtaa gaccaatccg
     7861 aataaaagca aacaacctaa gctattaaaa aagataatta aatataagct ggtatatttt
     7921 ttgatacttc cttcagttat ttgttatata atttttgctt atctgcctat ggcaggtgtt
     7981 ttgatggctt ttaaagatta ccaggtaggc aggggattca agggtatatt tacaagtcca
     8041 agtgtagggt ttgaacattt ccagcgactg tttaaatcat attacttttt cagtatttta
     8101 aggaacacaa ttttaattag tttttataag attatttttt gttttcccgc tcctattata
     8161 ttcgctatat tattgaatga gctaagaatt acatatttta agaggtttgt gcaaaccgta
     8221 acatatctcc cgcatttttt atctacagtt gttgtaacag gtttggttct tgcaatgctg
     8281 tctccatcct acggactggt aaatgctgtt ctaggtacaa tgggcctgga acctgttcat
     8341 tttctagcaa acagcagata tttcagaagc attattgttg ccattgatgt atggaaaggg
     8401 acaggatgga gtgctgttat ttatcttgct gcaatatcat ctattgatcc acagctatat
     8461 gaatcagcaa cgattgatgg tgcaggaaag tttcggcaaa tatggagcat tacactacca
     8521 tcattgttag atttgataat attactccta attttgagaa taggagatat attaaatgca
     8581 ggttttgagc aaatatttct gctgtattct cctcctgttt acagtgtagg agacattata
     8641 gatacctttg tttacaggga aggactagta aattcaaatt atggtttttc aacagcagtg
     8701 ggtcttttta aatctgttgt cggacttgtg ttgattgtcg gttccaacaa actggctaac
     8761 aggtttggta aacaaggtat atggtgattt tgatattatg taaacaaagg gggtgctttt
     8821 ttggtcaaac agtccaaagg tgaaaaaata tttaacttta taaatattct tattatgctt
     8881 attgcagtaa ttgctgttat aataccactt tttagcgtgg tttccacttc attagtttct
     8941 aatcaggagt tgtcaagaag acgtttcatt attttccctc aaaaggttga ttttacagct
     9001 tataaaataa tttttgaagg aacaagcata attattagtg ggtacaaaat aactatattc
     9061 agagtagttg tgggtacggt tattaatttg attttttcct actttactgc atatgcaatg
     9121 tcgaaaaagg atttacctgg cagaaatgta attactatct tctttttctt tacaatgctt
     9181 tttggaggcg gtctgattcc ttattatatt gtgataaagt ctgtaagatt gttaaataat
     9241 gtttgggttt atattttgcc tcaaattata tctgtatgga atactcttct tattaggaac
     9301 tttattatga atattccgga aagtcttgag gaatctgcag aaattgatgg ggcaaatgct
     9361 gtacatataa tattcagaat tattttgcca ttgtcgatac cagctcttgt aactataggc
     9421 cttttttacg ctgtaggtca ctggaatagc tggtgggatg ctttcttatt tgtaagtgat
     9481 gtcaggaagc agccgataca gttggtatta agaaatatcc ttgcatctgc ggaaatgagg
     9541 ataaatacta ttacaggcag aattgatgca actgaaataa aaccgccttc gcgtgcaata
     9601 caaacagcaa ctatagttgt tacgacagtt ccaatagtta ttatttatcc tttcatacag
     9661 aagtatttta ttaaaggtat aatgatagga gctgtaaagg ggtaaaaacg caggtactgc
     9721 ccgtgtgtat tcaaaaacta ttaatataca ttaatataaa ttaataaaaa aggagagaat
     9781 gattatgtta aagaaaaggt tattaactat gcttttatgt ctggtattaa tagcatcatt
     9841 gtttacagca tgcggaaaat ctggtaccaa cgctgagaaa accgatggtt cggatacgtc
     9901 cccactgaaa gaaaacaagg aagaaactgt aaccattgta atggagagac ctttatggca
     9961 aaatgctgat ccatcatctg atcctaatat gagaattagg caaaaaatca aagaaaaaat
    10021 caatattgat gtggaagtag tagggcagca gaatccgcca gatcaggccg aaaagcctaa
    10081 tctgatgatt gctgcaggtg aaactcttga tatttttcaa acacctttcg gagggtctaa
    10141 tgattggaga aagtataaga gagaagacac aattattcca cttaacgatc tactaaatga
    10201 atttggtgta gatctgctaa aacatgttaa tcaagatgca ttaaaagcat gcactgatga
    10261 agatggcaaa atatgggctc tgcctgatga acaaaatcca gtaacaacct tcttaatgat
    10321 gcgaaaagac tggcttgata agtataattt aagtgtacct gaaacatttg aagaatatga
    10381 aaatgtactt caagtattta aggaaaagga aaatgatgaa ggaatcgtac ctttttggca
    10441 gggacttgag gacaatatat ttgcagggtg cttcattcct actggagatc aaaaattttt
    10501 ggatagcgac ggaaaactaa aaccgaattt tatgcatcca aactataagg aatttctggc
    10561 taaaatggca gattggtata agaagggctt tttacataag gaatttgcta ctatgcaata
    10621 ccagcaggca gcagatttag cctttggagg acgctcagga ttatttgtca actggatcag
    10681 tggttcattt aagtttaatg ttgatacgct taaaaattct gtgcctgatg gagaattaat
    10741 tgtgatagct ccaccaaaag gacctgctgg aagacttata aggaaaggac taccaataac
    10801 gagtaatatt atgattacca aaagcagtaa gtatccggaa aaatcaattg aattccttaa
    10861 ctggagtatg gcaacagatg aaggatggct gcttagtaga tttggagaag aaggctatga
    10921 ctggaactgg attgataaaa gtaaaaatat acttgattat acagataaaa aggtagatgt
    10981 tgatagatat ggatatgcaa tattttgcag caccaggctc aataaatttg taaacaagta
    11041 taatattgga ggtatggtag attctgaagc tacagcattt gtacatgatg aaaataaata
    11101 tcctactaaa aatcctattg atttgcttgt aatttatgat gttaataaaa tgaagtcgaa
    11161 agacaagtta aattcaatga acacattttt aaatgaagca aaatatcaaa ttattatggg
    11221 tcaagaacag gtttctagtt gggataatgt aataaaaaaa tggcttgagc ttggtggtaa
    11281 ggaatatatt gatgacataa cagagcagtt taatgctcag aaataacgaa cattgctata
    11341 tgtagaaaca aaaaagggga ttgatactcc ccttttttgt tgtatacgat atactattat
    11401 tctcaaaaag ttgacaaaaa aacatttttg ctcaaagcca tgtagtataa gcgttatata
    11461 gcactttttg agaatatata gtaccttaat ttatcgtagc gtacgcttat ttgtttgcag
    11521 ctatacgctg cgatatgtat attacgttaa gaagaattat taaatttact ttgtacggac
    11581 aatctaatta agggggctta tatgtgtgag tatagaattt gaaaagatat ttgaaaatga
    11641 tgttttatca aaaagagaaa gagtagaacg aattcttaac catcaaccag ttgaccgtgc
    11701 agcact
//
LOCUS       DAOSDD010000137        11682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2125_length_11682_cov_5.704911, whole genome shotgun sequence.
ACCESSION   DAOSDD010000137 DAOSDD010000000
VERSION     DAOSDD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2125_length_11682_cov_5.704911"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="PK733_12165"
     CDS             <1..>1113
                     /locus_tag="PK733_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11331.1"
                     /translation="KYLPNNAKLCFGSREAPPQDFLSFKVKGNITELTQKELAFTREE
                     IVGILGFDDPAIYGSTEGWPLAIRSFQVLLENGISIGDITSYGNETLYAYLFRECIAN
                     LNSDMVDFLKKSACFDVLDAQMLDDVLNKKNTRLMLESLVSRNIFTIKTGDGFYRYHA
                     LFRSGLLETGDNDQMPLLRQKAAQYYFDHKQYSRAARYAIESKDNELLENIILACYRD
                     YIKAGNYNELRIWFRVLNDAAVELNPRILVAKGAFLSVLGNFVQAKACLEAAIPLMSR
                     DDKELYFEAMIHKARVLRNFVSFEESNRLLDELLARLDDQTGELAYSVVIEKLYNLCW
                     NSQIGEAYALARHMIESCANAGNIKIMRWFERYLCTI"
     assembly_gap    1114..1213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1214..1561
                     /locus_tag="PK733_12170"
     CDS             <1214..1561
                     /locus_tag="PK733_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11332.1"
                     /translation="DQGATKEQIYNAIWWESESNNIKNLIAVNLRHIKNDLECAGIME
                     SVIYRENRYFICRDEIAYEEFKLHNTKDQAKKLLSLYKGEYLADFEALWAIGNRIKYR
                     EAYKSALNFSRNL"
     gene            complement(1580..1708)
                     /locus_tag="PK733_12175"
     CDS             complement(1580..1708)
                     /locus_tag="PK733_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11333.1"
                     /translation="MRIPRKFGERTAHFGKTQDKKRKYVLAEDMRCCLYDNIQYKY"
     gene            complement(1711..3135)
                     /locus_tag="PK733_12180"
     CDS             complement(1711..3135)
                     /locus_tag="PK733_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOJ11334.1"
                     /translation="MDIMVSIVFVLSNIIGITSCFIKKVKLSLCIRIKQKKFDYNIYK
                     RKPKSKSERVLDADKLKLLKFATVFGANASGKSNLISSFKYAQTAIVLGVPKKGQASY
                     FKIDASYANKPSYFEFEILLNNNNYAYGFEIFLEKSQIVSEWLIQLSSTSNDKTIFSR
                     DVLSGKFQSDLKVTSQTLRNKFNVYSDDSKNNTEILFLNEMNRAKAELYKDESELFIF
                     KEVLDWFVDKLDVNYPNRPISDYSYFRDVKNKEEVCRVIRSLGTGITNYDERKSSLIQ
                     LQQQLPKVIYERLEEDIKEWKIEKIKDKKASSMSVRINDMYYVISLDKNDKIEVTTIV
                     FNHGNDAVFEFSEESDGTRRILDLIEILFTVEDKVYIIDEIDRSLHPQLTYKFIQEFL
                     VAAQKRNIQLVTTTHESRLLDFGLLRQDEIWIANKLTSGETELYSLDEYNVRFDKKVD
                     KAYLEGRYGGVPIFSTIFPIKEDD"
     gene            3666..3944
                     /locus_tag="PK733_12185"
     CDS             3666..3944
                     /locus_tag="PK733_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11335.1"
                     /translation="MNIYSNKIPKSVVEKLVCAYGNKNTADNLKEEIINDPLNAIGNI
                     NATGVKNVNIKTNDSLKFQASLKLMLKVIGELEAFFELKTVKKAEFKI"
     gene            4142..4393
                     /locus_tag="PK733_12190"
     CDS             4142..4393
                     /locus_tag="PK733_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11336.1"
                     /translation="MKKHSTSRRTLEYIKAVELAAKNLKERPTLAGMIGPFSLAGRLF
                     DMTEIMVNIMVEPDGVMKNSSVDEVLKKTEELLNRYRLQ"
     regulatory      4468..4652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4734..7043
                     /gene="metE"
                     /locus_tag="PK733_12195"
     CDS             4734..7043
                     /gene="metE"
                     /locus_tag="PK733_12195"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOJ11337.1"
                     /translation="MQKISVVGYPRIGIRRELKKWVEGYFKGKISKEQLLKNSAKLRV
                     QQWLLQKDKGIDFIPSNDFSFYDMFLDTACLLGVVPERYRELGLDYLDTYFAMAKGFQ
                     AGSRDVKALPMKKWFNTNYHYLVPSISDPTNFQLSSSKPLDEYAEALRSGVKTKPVII
                     GPLTFLKLSNITEKEKMYFDYADSILRVYKQLLFKFNELGIELVQIDEPVLVTDLSYE
                     DISCFTAMYKSILTEKGNVKILLQTYFGDIRDIYNEIMFLDFDAVGLDFVEGEKNIDL
                     IEEYGFPKNKLLFAGVVNGKNIWINNHRKTLEKLNLLEKHINKGSIVLSTSCSLLHVP
                     YTIKNETLLEERYIKQFAFAEEKLEELKELSELWDLDYYTLNKKYVKNVLNVIEKHES
                     KEFFDEIVREDVASLIEDDFIRYPVFEERIKIQKERFKLPLLPTTTIGSFPQTVEVRE
                     LRKKYKNGEITLEQYNEKIKYKIAEAVQIQEEVGLDVLVHGEYERNDMVEYFGENLSG
                     FLFTQNGWVQSYGTRGVKPPIIFGDVKWVKPITSEYIMYAQSLTDKSVKGMLTGPVTI
                     LNWSFPREDLPLREIAYQIALAIRQEVKDLEAKGIKIIQIDEAALREKLPLRKEDWHN
                     EYLDWAIKAFRLVHAHAKPQTQIHTHMCYSEFKDIIKEIEEMDVDVITFEAAKSDLSL
                     LDILKESNFRPEVGPGVYDIHSPRVPDIDEIKEIILKMISRLNIEKVWINPDCGLKTR
                     GMDETTTSLKNMVAAVEDIRKEIKYLQNK"
     regulatory      7090..7288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7424..7621
                     /locus_tag="PK733_12200"
     CDS             7424..7621
                     /locus_tag="PK733_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11338.1"
                     /translation="MPGMVSLSTKPPVLEWIYTFDLERDIGQGQFYCIVGGNGTGKTT
                     ALFLISGLTTFFIYGGIINVG"
     gene            8077..8316
                     /locus_tag="PK733_12205"
     CDS             8077..8316
                     /locus_tag="PK733_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOJ11339.1"
                     /translation="MTLDKLPVGETAIITRVGGEGALRCRLLDMGIIPKTKVVVCKVA
                     PLGDPIEIRIRDYTLTLRMEDAEKIEIMHAEAYRL"
     gene            8313..10319
                     /gene="feoB"
                     /locus_tag="PK733_12210"
     CDS             8313..10319
                     /gene="feoB"
                     /locus_tag="PK733_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ11340.1"
                     /translation="MIFALVGNQNCGKTTLFNQLTGSNQHVGNFPGVTVEQKVGKIRS
                     SKNVSVVDLPGIYSIRPYTNEEVVTRDFILNNKPDGIINIVDATNIERNLYLTLQLIE
                     MNIPMVIALNMMDEVRGNGGTVDVGKMSNELGIPIVPVVASKNEGIGELTDIMLNTAR
                     NKICPKKIDFCESGPIHRCIHAVSHHIEDHADKCGMAARFAATKIIEGDPDIILKLEF
                     NQNELDMIEHSIVEMETEHGMDRNAALADMRYDFIEKVCAHTVVKCKESKEHIRSVKI
                     DNILTNKYLAIPIFLVMMFTIFWLTFSVIGTFLSDLLASGITALTNLVDAGLKLYGIN
                     PVVCSLVIDGIFAGVGSVVSFLPIIVTLFFFLSVLEDTGYMARVAYVMDKLMRKIGLS
                     GRSFVPMLIGFGCTVPAVMSARTLPSERDRKMTIMLTPFMSCSAKIPIYAVFTAAFFT
                     KYKALVMIGLYVTGILVGILAALISNKTVCRGRPVPFVMELPNYRFPSAKSVLLLMWD
                     KARDFLQKAFTVIFVATIIIWFLQTFDSRLNVVTDSSYSILAMIGKLIAPAFIPLGFN
                     DWRVATALITGFTAKEAVISTMSVLTGTSVTELSTTLSNLFTPLTAISFLVFTLLYTP
                     CVAAIAAIRRELNSGVLAIVVVAAQCSVAWVTAFAVYRLCSLIF"
     gene            10502..10879
                     /locus_tag="PK733_12215"
     CDS             10502..10879
                     /locus_tag="PK733_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOJ11341.1"
                     /translation="MKILEAAENYLEVILIENEKHGQVRSIDICNSLNYSKPTVSVMM
                     KRLRENGYIEMDQDGYITLTDKGSSIAEKMYERHMILAEVLTALGVDEETAYKDACKI
                     EHDISSTSFEAIKKYYYKRKGKD"
     gene            11144..11422
                     /locus_tag="PK733_12220"
     CDS             11144..11422
                     /locus_tag="PK733_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOJ11342.1"
                     /translation="MPRRDGTGPMGSGSMTGRGLGLCTGANAVKYGAGIGMGLGLGLA
                     CRRGFGRGFGRGFAVNQTSSKTQKELLQEQKNMLQDRLKVIDKQLENL"
     gene            11487..>11682
                     /locus_tag="PK733_12225"
     CDS             11487..>11682
                     /locus_tag="PK733_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOJ11343.1"
                     /translation="MARPMKWRKVCCLPESSRFGPLDSPIDAKNFVNMTVDEYETIRL
                     IDLEGFTQEECANQMNVARTT"
BASE COUNT         3777 a         1869 c         2444 g         3492 t
ORIGIN      
        1 aagtatctcc ctaacaatgc caagctttgt ttcggcagca gagaagctcc cccacaggat
       61 tttttatcgt ttaaagtaaa gggcaacatc acagaactga cccaaaagga gcttgcgttt
      121 acaagagagg aaatcgtcgg tatcctgggt tttgatgatc ccgctattta cggttccacg
      181 gaggggtggc ctctggccat ccgttctttc caggtattgc tggagaacgg tatatccatt
      241 ggcgacatta cttcctacgg taatgaaact ttgtatgcct accttttccg tgaatgcatc
      301 gcaaatctga actccgacat ggtggatttt cttaaaaaat ccgcttgctt tgacgtgctg
      361 gacgcacaga tgctggatga tgtcctgaat aaaaaaaata caagacttat gctggaaagc
      421 cttgtatcca gaaatatatt caccattaaa acaggcgacg gattttatcg ctaccacgcg
      481 cttttcagaa gtggcctgct ggaaacggga gataatgacc aaatgccttt actgaggcag
      541 aaagccgccc agtactattt tgatcataag cagtattcaa gggctgcccg gtacgccatt
      601 gaatcgaagg ataacgaact tctggaaaat ataatcctcg catgctatag ggattacata
      661 aaagccggca attacaacga gcttcggata tggttccggg tgcttaatga tgcggcggtc
      721 gaactaaacc caagaattct tgtggccaag ggtgcgtttc tgtccgttct cggaaatttt
      781 gtacaggcca aagcatgcct tgaagcagcg atacccttaa tgagcagaga cgataaggag
      841 ctttattttg aggctatgat acataaggca agggttctcc gtaatttcgt ttcattcgag
      901 gaatcaaaca ggctgcttga tgagctttta gcaaggcttg acgatcagac tggggaactg
      961 gcatattctg tagttatcga aaagctgtac aacctatgct ggaattcgca gataggcgag
     1021 gcctatgctc ttgcacggca tatgatagag agctgcgcca atgccggcaa tattaagatc
     1081 atgcgttggt ttgaaaggta tctttgcaca attnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnngaccagg gggctacaaa agaacaaata tataacgcta tttggtggga
     1261 atcggaatcc aataatataa aaaacctgat tgcggtaaac ctgaggcaca taaaaaatga
     1321 ccttgaatgt gccggcataa tggaatcagt catataccgg gaaaaccgct attttatctg
     1381 ccgggatgaa attgcatatg aagaatttaa gcttcataat accaaagatc aggcaaaaaa
     1441 gcttctatct ctatataaag gagaatactt agcagatttt gaagcacttt gggctatcgg
     1501 taataggatc aaataccgtg aggcttataa atcagcatta aactttagta ggaatctata
     1561 acctcatcta ctttttgctt taatatttat attgtatatt gtcataaagg cagcatcgca
     1621 tatcctctgc aagaacatat tttctttttt tatcttgcgt ctttccaaaa tgtgctgttc
     1681 tctccccaaa ttttcttgga attctcacaa ttaatcatcc tccttaatag ggaagatagt
     1741 actaaatatg ggtactcctc cataccgacc ttccaaatac gctttatcca cctttttatc
     1801 aaatcttaca ttgtattcat caagagaata aagctctgtt tccccagacg taagtttatt
     1861 ggcaatccat atttcatcct ggcgtaataa tccaaagtct agcagtcttg attcatgggt
     1921 tgtggtaacc aactgaatat ttctcttctg tgcagcaact aaaaattcct gaataaattt
     1981 ataagttagc tgtggatgta agcttctatc tatttcatca ataatatata ctttatcttc
     2041 tacagtaaaa agaatttcga tcaaatctaa aattctcctt gtaccatctg attcttcaga
     2101 gaattcaaaa acggcatcat ttccatggtt aaaaactatt gtagtaactt ctattttatc
     2161 atttttatct aaagaaataa cgtagtacat atcatttatc cgtacagaca ttgaagatgc
     2221 ttttttatct tttatctttt caattttcca ttctttaata tcttcctcta atctttcata
     2281 aatcactttt ggtaattgct gctgaagctg tatcaaagat gactttcttt catcgtagtt
     2341 ggttattcca gttcctaaac ttctaattac cctgcatact tcttccttgt tttttacatc
     2401 cctgaaatag gaataatcag aaattggtct gtttgggtaa tttacatcca atttatctac
     2461 aaaccaatca agcacttcct taaatatgaa taactcactc tcatctttat acaattcagc
     2521 ttttgccctg ttcatttcat ttaaaaagag tatttctgta ttgtttttgc tatcatcaga
     2581 ataaacatta aatttatttc ttaatgtttg tgaggtaact tttaaatcag attgaaattt
     2641 gccacttaaa acatctctac taaaaatagt cttatcattg ctggttgaac ttaactgaat
     2701 taaccactca gatactattt ggctcttttc caaaaaaatc tcaaagccat atgcatagtt
     2761 gttattgttt aatagtattt caaactcaaa atatgagggt ttgttggcat agctagcatc
     2821 aattttgaaa tatgaagctt ggcctttttt aggaacgccc agaacaatag ctgtttgagc
     2881 atatttgaag ctggaaatca gattggattt acctgatgca tttgctccaa aaacagttgc
     2941 gaactttaaa agctttaact tatcagcatc aagtacccgc tcagatttac tctttggctt
     3001 tcttttgtaa atattataat caaatttttt ctgttttatt cttatacaaa gtgataattt
     3061 caccttttta atgaaacaag aggttattcc aataatattt gataaaacaa agactatgct
     3121 taccattata tccatttact tttatacata tattaaaatt tgctttaata agaattatcg
     3181 aaaagaaata ttactaaaaa atcaaggcaa aaaatttaat aagactacct gatgaataat
     3241 ataagatttc tttttgatta taagatgcat ggaatgagca gcattggaga aataaaaaga
     3301 agatagagga aactatctct gataaaactg ttttgaagct ttggacggtt tcattaattc
     3361 ccgatgaggc aataacagca gatatcagat gaacactctt cagacataac taagctgctt
     3421 caaggctgtg cagaaaaaga gtacaaaaga aaaggagcga tagcatagag ttcatttggt
     3481 actttgataa atcgataata taaattcaat tgacagccga tttatataga aatagaaatg
     3541 gttttcaggg ttaatctgaa cggcttaatg acaatgttac ggcaatggtg tcagacaaaa
     3601 tattatgcca ttccattgcg caaaatattt cacgtattat gaaggagtac cagcatactt
     3661 ttgcaatgaa tatttattca aataagattc caaagtctgt agttgagaag cttgtttgtg
     3721 catatggaaa taaaaatacc gccgataacc tgaaggagga aataatcaat gaccccctaa
     3781 atgctatagg aaatataaat gcaactggtg taaaaaatgt aaacattaaa actaacgata
     3841 gtcttaaatt ccaagcttca ttaaagctta tgttaaaggt aattggggaa ctagaagcct
     3901 tttttgaatt gaagacagtt aaaaaggcgg aatttaaaat ataggcaaga ccatgtagac
     3961 cggaacatgc attgtaatat ataaatttga ctaaaataat ttatttctaa tggaacctga
     4021 tataatttat gttataaata ttattttctc attaagatat atatatagcc tacaaataaa
     4081 ggatttgaag tggtaaactt tcacttagca aaaaacatga catttcactt ttttaatggt
     4141 aatgaagaaa catagcacat ccaggcgtac gcttgaatat ataaaggctg tagaactggc
     4201 agcaaagaac ctgaaagaaa gacctaccct tgcaggtatg atcgggccat tttctttagc
     4261 aggccgtttg tttgacatga ctgagattat ggtgaatata atggttgagc cggatggtgt
     4321 tatgaaaaac agtagcgttg atgaagtttt gaaaaagact gaggagcttt taaataggta
     4381 tcgactccaa taaattgtat ataagtacca aaattgaatt tatatattta tttatttgct
     4441 attgacaagg caaatagatg taaataaaat aggaacaagc tataaggtgc ccctgctaaa
     4501 aggggagaat agggaaccgg gtgaaaatcc agggcagctc ccactactgt gagcttgacg
     4561 aaattccttc ataccactgc tgtaaaggcg ggaaggctgg aaaagtagga tgaaagcaag
     4621 ccaggagacc tgccttatat gccaagctca atctttgggt atggagatag aggcgatttg
     4681 tagtcaaatt gtatctattt cctccataaa ttttatttag gaggaagaag aatatgcaaa
     4741 aaatttctgt agtcggttat ccaagaatag gtatccggag ggaattgaaa aaatgggtgg
     4801 aaggatactt taagggaaaa atttcaaaag aacaactgct aaaaaattct gcaaaactaa
     4861 gggtgcagca atggctgctt caaaaggata aaggaattga tttcattcct tcaaatgact
     4921 tttcatttta tgatatgttt ctggacacag catgtctact tggtgttgta cctgaaaggt
     4981 acagggaatt ggggctggat tatctggata cctatttcgc catggcaaaa ggttttcagg
     5041 ctggatcacg agatgtaaaa gcgcttccta tgaaaaagtg gtttaatacc aactatcatt
     5101 atctggttcc atcaatcagt gatccaacta attttcagtt aagtagcagt aaaccgctgg
     5161 atgaatatgc agaggcattg aggtctggag taaaaaccaa gcctgtaatt attggaccgc
     5221 ttacatttct taagctttcc aatattactg aaaaggagaa gatgtatttt gactatgctg
     5281 attcaatact acgtgtatat aaacagctgc tattcaaatt caatgaattg ggtatagagc
     5341 ttgtacagat agacgagcct gtcctggtaa cagacctttc ctatgaagac ataagctgct
     5401 ttactgccat gtacaaatca atcttgactg aaaaaggcaa tgtaaaaata ctgttgcaaa
     5461 catacttcgg agacataaga gatatataca acgagattat gtttctggat tttgatgctg
     5521 ttggattgga ttttgttgaa ggagaaaaga atatagactt gatagaggaa tatggctttc
     5581 cgaaaaacaa gcttttattt gcaggagtag taaacggaaa aaatatctgg atcaataatc
     5641 atagaaaaac acttgaaaag ttaaatttat tggaaaagca tattaacaag ggaagtattg
     5701 tgttaagcac ttcctgctct ttgctgcatg tgccttatac aataaaaaat gaaacactgt
     5761 tggaggaaag atatattaag cagtttgcct ttgcagaaga aaagctggaa gaattaaagg
     5821 agctttcaga actctgggat ttggattatt atactttgaa taaaaaatat gttaagaatg
     5881 tccttaacgt catagaaaag catgaaagta aggagttttt tgatgaaata gtgagggagg
     5941 atgttgctag tttaattgaa gatgatttta tcagatatcc ggtgtttgaa gagcgaataa
     6001 aaattcagaa agaaaggttt aagctgccgc tgcttcctac gaccactatt gggtcttttc
     6061 cacaaacagt tgaagtgagg gaattgagaa aaaagtataa aaatggtgaa attacattgg
     6121 agcagtacaa tgagaaaata aagtataaga ttgccgaggc tgttcaaatc caggaggaag
     6181 ttggtttaga tgtactagtt cacggtgaat atgaacgcaa tgatatggtt gagtactttg
     6241 gagaaaacct gtcgggattt ttgtttacac aaaacggatg ggttcaatcc tatgggacca
     6301 ggggggtaaa accgccgatc attttcggag atgtgaaatg ggttaagcct ataacttcag
     6361 aatatattat gtatgctcaa agtttgacag acaaatcggt gaaagggatg ttgacgggac
     6421 ctgtaaccat attgaactgg tctttcccaa gggaggattt gcctctgaga gaaattgcat
     6481 accagattgc actggcgatt aggcaggaag taaaagacct cgaagcaaaa ggtataaaaa
     6541 ttatacaaat agatgaagct gctttaagag aaaaacttcc ccttcgaaaa gaggattggc
     6601 ataatgaata tctggattgg gcaatcaaag cattcaggct tgtccatgcg catgccaagc
     6661 cccaaacaca aatccatacg catatgtgct acagtgagtt caaagatata atcaaggaaa
     6721 tagaggaaat ggatgtcgat gtgattacct ttgaagctgc taaatctgac ttatcacttc
     6781 tggatatttt aaaagaaagt aattttaggc cggaggtagg accgggggtg tatgatattc
     6841 attcaccaag agtaccggat atagatgaaa tcaaggaaat aatattaaaa atgataagta
     6901 ggcttaatat agaaaaagta tggataaatc ctgactgcgg gctaaaaaca agagggatgg
     6961 atgaaactac taccagctta aaaaatatgg tagctgctgt agaagacata agaaaggaaa
     7021 taaagtattt acaaaataag taaaatttca catgataaga agtgtttaat gagatatagt
     7081 agatacgaaa actaaatatt agaaacaggt gccaattatg tttttacgga taaatacatg
     7141 aattggtgaa aagggaagtg ggttgaaatc ccacgcggtc ccgccgctgt gaaagaagag
     7201 tccttgcagg atgccactgg gaaaccggga aggctgtaag ggtgatgatg cttgagccag
     7261 aatacctgcc tgttttgtta caccattaaa ctctacgagc gatagggagg tgcaggatat
     7321 attatgtaaa tcataataat tattaaaaac cttctggcag cttttgttgg aaggttttat
     7381 ttgtaaagaa caattatgag tagctttgaa ggtgaggtga aatatgcccg gaatggttag
     7441 cttgtccaca aagccgccag ttctggaatg gatttacacc tttgatttag agcgtgatat
     7501 tggccaggga cagttttatt gtattgttgg aggaaacgga acagggaaaa caactgcttt
     7561 gtttcttatc agcgggctga ctaccttttt tatttacgga ggaattatta atgtcggtta
     7621 gctgttgatt tttacgtcac aattttcctg gaaggctttg cttaccacct atatttcagg
     7681 attttggttc gacatgatgc acgctatcgc tacagtattc ttcctatacc ttatttctca
     7741 gcctatgatt gaaaaattgg agcggattaa aataaagtat gggctaatcg aatcatagga
     7801 tgaagcagga ctaatagtgc agatgatatg ggtgctgaga ttttaactga agggaataaa
     7861 agaaaaagat gcagcatttt agttttcagc taatgatcta aaagaggtat tgtcataacc
     7921 tgagtactat tttcatactg gctatatgta gctggatgaa tttaaaaatt tgatagtata
     7981 ttgacaattc caggcagtta ggatatacta accttacgga gttagtatat cctaaccaag
     8041 gaaacctata tatgatacaa agggggttgc aataatatga cactggataa gttgcctgta
     8101 ggagaaactg caataattac aagagtcggt ggcgaaggag ccttgcgttg caggcttttg
     8161 gatatgggga taatacccaa aacaaaggtt gtagtttgca aggttgctcc tttaggtgat
     8221 ccgatagaaa tacgaatcag agattacacg cttacacttc gtatggaaga cgctgaaaag
     8281 atagaaatca tgcatgcgga ggcttataga ttatgatatt tgcattggta ggaaatcaga
     8341 attgcggtaa aactacgctg tttaatcaat taactggttc gaatcagcat gtgggaaatt
     8401 ttccgggtgt gactgttgaa caaaaagtag gaaagatccg ttcttcaaag aatgtatcag
     8461 tagtggactt gcctggaata tattccattc gcccatatac aaatgaagag gtcgtaacgc
     8521 gtgactttat tcttaacaac aagcctgacg gcataataaa tattgtagat gctaccaata
     8581 tcgagcgcaa tctgtacctt acattgcagc ttatagagat gaatatacca atggtgattg
     8641 cattaaatat gatggatgag gtgaggggta acggtggtac ggtggatgtt ggtaaaatgt
     8701 ctaatgagct gggaatacct attgtgccag tagttgcttc aaaaaatgaa ggtattggtg
     8761 aattaacaga tataatgctt aacactgcca gaaacaagat atgtccaaag aaaattgact
     8821 tttgtgagtc tggtcctatt cacagatgta tacatgccgt ttcccatcat attgaggatc
     8881 atgctgataa atgcgggatg gcggccagat ttgcggcaac aaagattatt gagggtgacc
     8941 cggatattat attgaagttg gagtttaatc aaaatgaact agatatgatt gagcacagta
     9001 ttgttgaaat ggagactgag catgggatgg acagaaatgc ggctcttgcg gatatgcgat
     9061 atgattttat tgagaaagta tgcgcacata cagtggtcaa atgtaaggaa agcaaggaac
     9121 atatcagaag cgtaaaaatc gataatattc ttaccaacaa atatcttgct attccaatat
     9181 tccttgtgat gatgtttaca attttctggc ttacattcag tgtaattggt acctttttaa
     9241 gcgatcttct tgcatcgggc ataactgcac tgacaaacct ggttgatgcc ggactgaagc
     9301 tatatggaat aaaccctgtt gtgtgcagcc ttgttataga tgggatattt gcaggagttg
     9361 gaagtgtcgt gagttttctt ccgattattg taacattgtt tttctttctt tccgtccttg
     9421 aagacaccgg ctacatggcc cgagtagctt atgttatgga caaactgatg cgcaaaatcg
     9481 gtttgtcagg cagaagcttt gttccgatgc taataggctt tggctgtact gttcctgcgg
     9541 ttatgtctgc ccgtactctg ccgtcggaac gagatcgcaa aatgaccatc atgttaactc
     9601 catttatgag ttgttctgca aagattccaa tatatgccgt atttacagct gcttttttta
     9661 ctaaatacaa agcactggtg atgattggac tttatgtcac gggtatacta gttggtattt
     9721 tggctgcgct tatttccaac aagactgttt gtcgaggaag gcctgttcca tttgttatgg
     9781 aacttcctaa ttatcgtttt ccatctgcaa agagtgtatt gcttttaatg tgggacaagg
     9841 ccagagactt tttacagaaa gcatttacag tcatatttgt ggcaacaatc atcatttggt
     9901 ttttgcagac ctttgatagt aggttaaatg tggttacaga cagttcatat agtatccttg
     9961 cgatgatagg caaattgatt gcacctgcat ttatacctct gggctttaat gactggagag
    10021 ttgctactgc actgattaca ggatttactg ctaaagaagc tgttatcagt actatgagtg
    10081 tcttgactgg tacatcagtt actgaactta gtacaacctt aagtaatttg tttactccgc
    10141 tgactgcaat tagctttctg gtattcactc tgctttatac gccgtgtgtt gcagctattg
    10201 ctgcaatcag gagagaactt aattcggggg tactggctat agttgtggtt gcggcacaat
    10261 gcagtgttgc ttgggtcacg gcatttgcag tctatcgtct ttgcagcctg attttttagt
    10321 ggtgattata tgaaaattat ggattacttt gttgttttac taattttaat atggttaatt
    10381 atggctctgt gttatataat aaggataaag aagcagggca aaagcatatg ctgcagtagc
    10441 tgtagatcat gttacaacaa ctgccgtata tgtagacaga aaaatttagg agttgaattt
    10501 gatgaaaata ctcgaagcag cagaaaacta tcttgaggtc atattaatag agaatgaaaa
    10561 gcatggtcag gttcgttcaa ttgacatatg caattcgctg aattattcca agccgacagt
    10621 cagtgtgatg atgaagcggc ttcgtgaaaa tggctatatt gaaatggacc aggacggcta
    10681 tatcacgctt acggataagg gcagcagtat tgcagaaaaa atgtatgagc gccatatgat
    10741 attagctgaa gtattaacag cattgggcgt cgatgaagaa actgcttata aagatgcatg
    10801 caagatagaa catgatatta gttcaacaag ctttgaagct atcaagaaat attactataa
    10861 aagaaaaggt aaagactaat aagtcttaac cggtgaagca aaatcattga tgttgaatgt
    10921 gcttctgctg ggctttgcct gccaacatgc tgaacagatg aaggctgtaa ttaaatgtat
    10981 aactgaattg gaacgtggag ttcttataaa aaagcaaaac atcattcttt gtgatgcttt
    11041 gctttttttg taaaaacgaa aatagttatt gacatatgtc agaaatgtag tataataaga
    11101 atagaaagtg acatatgtca gaaactataa ggaggtgaat ggtatgccaa gaagagatgg
    11161 aaccggccca atgggttctg gatcaatgac tggaagaggt ttaggacttt gcacaggtgc
    11221 taatgcagta aagtatggag ctggtatcgg aatgggatta ggactaggac ttgcttgcag
    11281 acgcggtttc ggtcgtggtt ttggtagagg ttttgcagtt aaccagacct cctcaaaaac
    11341 acaaaaagag ctgctccagg agcaaaaaaa tatgttacaa gaccgactca aagttattga
    11401 taagcaatta gagaacttat aactggtatc agaggtaact ggaggacgct ctggttattt
    11461 ctgattagaa ttgaggtgaa aaaattatgg caagaccaat gaaatggaga aaagtttgct
    11521 gcttacccga gagcagtagg ttcgggcctc ttgattcacc tatagatgcg aagaattttg
    11581 ttaatatgac agttgacgaa tatgaaacca taagactcat cgatttagaa ggctttacac
    11641 aagaagagtg tgccaatcag atgaatgtcg ctcgtaccac tg
//
LOCUS       DAOSDD010000138        11637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2133_length_11637_cov_4.919876, whole genome shotgun sequence.
ACCESSION   DAOSDD010000138 DAOSDD010000000
VERSION     DAOSDD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_11637_cov_4.919876"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK733_12230"
     CDS             complement(<1..423)
                     /locus_tag="PK733_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ11344.1"
                     /translation="MAEIFLRFPEGRAKSLTLSYDDGVEQDIQLIEIMNRHGLKGTFN
                     LNSGLYAAEGTVYPKGQIHRRMTEKQTTETFINSGQEVAVHSLTHSFLEQLPIDMAVN
                     EIIKDRENLEKQFGTIVRGMAYPYGTLSDSVVEALKFCG"
     gene            complement(431..1291)
                     /locus_tag="PK733_12235"
     CDS             complement(431..1291)
                     /locus_tag="PK733_12235"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOJ11345.1"
                     /translation="MVKALTIGNSFADDATRYLEEIAAAAGKDVGLVIGKANIGGCSL
                     EKHWNLVEQCDLLPNVKPYNFYMTGKETCSATLKEALVSEKWNFVTLQQVSDLSWKTE
                     TFYPYIEKLYDLVEKFAPQAQMAIHQTWAYRCDAGELEKYGISQNEMFARLKRSYYDA
                     SKKLGCSVIPCGEAFQKARAIFNYTPDKNFDYVNAKPLELPDQSKSLIKGFFWKTGNT
                     ATGKAELSLDGRHGNEKGCYLANAVWYEMFTKNSIFDNEFCPEGVSQDELDILKNVAH
                     ETVIEYGEGV"
     gene            complement(1428..2306)
                     /locus_tag="PK733_12240"
     CDS             complement(1428..2306)
                     /locus_tag="PK733_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ11346.1"
                     /translation="MELNENKNFRSFTPYFANMHLHSTHSDGQFTPYQLAILAKSLGY
                     GGVVLSDHDVISGVRELTRLAEKIGIKSMSGVEFTCRQWGVGFHILGYDFDVDNIELI
                     TFVKRLCKYRNDHTRELLEAGLSRGTLKGITWQEVIDRNPENEWFCNDQVCRAMVDKG
                     IIHKEDWEEIHKVNFSYDSPYALPIKEASVEEAVKYIRKAGGIAVLAHPKNQYEYVDK
                     LVKIGIQGIEVCHPMLDEEDEKMFRRKAEQLKLYKTGGTDHTGPMGGYMGSYAVPAFH
                     GALEDDYIEIKERRFG"
     gene            complement(2369..3484)
                     /locus_tag="PK733_12245"
     CDS             complement(2369..3484)
                     /locus_tag="PK733_12245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ11347.1"
                     /translation="MTNIIIGADIVPTEFNMELFINGDVEALFGRGILDVLDKSDLRI
                     FNLETPLCDVKKPIPKCGPNLIAPTASVKGISAIKPSLLCLANNHILDHDEQALFKTL
                     EVLDKEGINHCGAGKNLEEAKKPFILHKNGLKIGIYTCAEHEFTIANDNSAGANPYEP
                     LVSFDHVSELKKVCDYVIVLYHGGREEYRYPSPNTQKVCRKFIEKGADLIITQHTHCI
                     GCYEDYKGGTIVYGQGNFMFIYGEDDSEEWQTSILVNIRISDDKKVTVDYIPLTRHDN
                     GMRLSNDEEGKEILDAFYRRSRELSEDPGFAKDKWGEYCSKTNMIERLVKNFTAIGDN
                     EDNIKANMVVYNMIHCEIHREVFEEIMFTRWKRFMQK"
     gene            complement(3537..5120)
                     /locus_tag="PK733_12250"
     CDS             complement(3537..5120)
                     /locus_tag="PK733_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ11348.1"
                     /translation="MKLQIFSENDWVYPDSEITELSDSICLDTARNANISFQVLTNLQ
                     VDDDTPISIEWSGEDVEGLTLIPYQLIPVRVEENSGVKLYTTLDYDSVKDFVTRQAPF
                     YVYDATRDIDDGMLKKGRAAFYFRISVLSKLEPGEYESVLKLIFNGCEGKINIKLRVY
                     KAVVPGLENCRFSMVNWLFLDHVSKAHNLEYGSEKFWDVIGHYMDNQLDMRSSHLMLS
                     SGKPILDENGNVVDFDFSEMIKEGKMAIDKGFTYIYGGFVSHWKKWDATELYLLWNMD
                     IEVASREAYRQLKIYFTKLWSIILENGWENKYMQCLVDEPNFPNSEHYRILSGICRKC
                     MPGVVINDPVESTELGGAVDIWVVKQSFYEQHKAEHELLQAWGEEIWIYTCGYPAGKT
                     MNRVIDLSLMASRLPMWMCYKYNAPGFLHWGYNVYSDEPFEYTCLNRGKPEELLPPGN
                     SNIVYPGNDKPWYSIRAHLQRAGAEDFELLYQLGLRDRNKADEIIARVCTTFDEYNPS
                     AENFNEVRRTLLEALDELL"
     gene            complement(5214..7172)
                     /locus_tag="PK733_12255"
     CDS             complement(5214..7172)
                     /locus_tag="PK733_12255"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ11349.1"
                     /translation="MFSANIDSYYDIECQLPDYLKKLAKQYFEAESNKKLGIHTIQQF
                     EACRRKTRDFFLSSIGGLENVKTPLNTICTGSLKYDGYTIEKIIYESYPGFYVTSNLY
                     LPETCSGEKHPGILFACGHSDNGKAEPAYQRVCIDLVINGFVVLAVDPLGQGERVQYY
                     DSETGTSSIGIGTREHSYAGLQCELTGSNIARYFIHDLIRGLDYLSSHPAVDPERLGM
                     CGNSGGGTQTSYIMLVDERLKAAAPSCYINSRENYMKTGQAHDNEQNIFGSIAYGLNH
                     DDFITAFAPKPVLINSARYDFFCIEGAVKSFERAKKIYEIYGKKDLIEMCISDSVHGY
                     SDVLRQSTVNFFRKHLKNQKPDFITSKNIPTEKDNNLLCTKSGQILTDYENAKTVFDL
                     NLEYFSNNRYKNEVGAEGGLEFIKERVIKVMNFPQPLDEREEIIYDRVVSDKVIDGRR
                     YEKIFFFSEEDIAVDGIYITPEGRELSEKNCTILLLEEGTNALENEKELINHLLDNGS
                     VFVFDPRGMGAAKSRKVNPRGYNDIYGTEYKLNSDAMMMGTSLAALRIYDVIRAHDYV
                     KRRLNGIIPGIAGKGVCSFYALVEAILVKDVQSIYLKDPMPSFEEIVKTKFYQYNCKY
                     DIFSVLKEFDIPWMIDELKKAGYSIIKL"
     gene            complement(7673..9430)
                     /locus_tag="PK733_12260"
     CDS             complement(7673..9430)
                     /locus_tag="PK733_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11350.1"
                     /translation="MKKLKKTIVIIMCVLLVFSAFSGCTSKKGNDSNSSEGTKEPDGG
                     KTEPQEVIELDFMYVGGWPGSGTEDTAILKKIEELTGVRIKYSFADKEKKQVIFAGGD
                     TTDCMVISSQTDILPAIQGNLILPLTELIDKYGPNVKKYFPERLKMAKDFLSDGSGEV
                     YVLPCHAGSEGRPASVYHSIYLTRWDYYKELGCPEIKGIDDYLDLLVKMVEAHPKTEE
                     GNPVYGISFWADRIFSFSHRFNYTYGYYHSNDHIVTHISDGKMVYNFTDENSPYWQAA
                     ELYNKAYRLGILDPDSFTQKSADFRAKVDMGQILTPVFDNYCDTFEKRMLENDPKSIK
                     GFQVIPVEGTTYWADSVYKGGWNSCFITIPKTCKSPKHVMKVINLLSDNDGARLAYSG
                     IKGEHWDIIDGKPALTDKTVKLYLDRAGESKEEFNSIGFGHFQSTLQTITAAGMADIC
                     PDGGLVDLFMSPDIYKLSNKPVDTDFSSYYGAEYPMGVFAKKIDEGTIFTHDNDVFDL
                     RVVTGMGTAPEDIARIDVRLNDMALKAIPKIVMAKSEDEFKQLKAETIKKMNEAGAVE
                     SRAWWQGRHDELTKIFVGK"
     gene            complement(9530..10417)
                     /locus_tag="PK733_12265"
     CDS             complement(9530..10417)
                     /locus_tag="PK733_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11351.1"
                     /translation="MKKNVSISSFVFDIINFVVLTVFGFLCFYPFYYMLIYSLSDPVE
                     ATKGVALLPRGLTLKNYWAVIHLEEIPLATVVSVFRTILAAVITIGCSSFLGYLVTKK
                     EMYFRKFIYRYVVATMYVGGGLIPTYLTLRAYGLRNTFFVYIIPGAISAYYVVLTKTF
                     FEQIPESLEESAMLEGAGILTCWARIVMPLSKPILATIAVFSLVAEWNYWFDSHIYIT
                     NDKLYTLQYVLYLYNQKAQSVADALKINPILSEDAMKITPATVRMTITAVVTIPILMV
                     YPFMQRYFVKGMMIGAIKG"
     gene            complement(10414..11346)
                     /locus_tag="PK733_12270"
     CDS             complement(10414..11346)
                     /locus_tag="PK733_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11352.1"
                     /translation="MNSHLCKKNKKEVKRINPYGLKLFLLALPFVVLVFMFRYVPLLG
                     WSLAFFNYKPGLSFDKMQFVGLKYFKLIGFFKEDVINALINTLVMSGLSLLTSPLPVI
                     FAILLTELRSSKFRKVLQTTVTLPHFVSWVIVYSLCFGLFSSNGLVNNLLLNVGSGKQ
                     FNLLANADKAWLFMLYLGIWKSLGWDSIIYLAAISSIEQTLYEAAKIDGAGRFRCIWH
                     IVIPGVMPTFIVLLLLQVSNILSAGFEKYFAFNNVMVAEKLEVLEMYIYRIGIGTQDY
                     SFATAVGIIKSAVSLTLLFSVNSLAKHVRGESLI"
BASE COUNT         3560 a         2488 c         1716 g         3873 t
ORIGIN      
        1 accgcaaaat ttaagagctt caacaacact atcactcaat gttccatacg gatatgccat
       61 gccacgcaca attgtgccaa actgtttttc gagattctcc ctatctttta ttatctcgtt
      121 aacagccata tcaataggca attgttcaag aaatgaatga gttaatgagt gtactgcaac
      181 ctcttgtcct gaatttataa aagtttctgt agtctgcttc tcggtcattc tgcggtgaat
      241 ttgccctttg ggataaactg taccttcggc tgcatacaat ccgctgttta aattgaatgt
      301 cccttttaat ccatgtctat tcatgatttc aataagctgt atatcttgct ctactccgtc
      361 atcataactt aatgtaagtg attttgccct gccttcggga aatcttaaaa aaatttctgc
      421 cataattcct tcacactcct tcgccatatt caattacagt ctcatgagcc acatttttca
      481 agatatctag ttcgtcttgt gaaacacctt ctgggcaaaa ttcattatca aatatactgt
      541 tttttgtgaa catttcatac catacagcgt ttgcaaggta acaacctttt tcattgccat
      601 gcctgccatc gagactcaat tcagcctttc ccgtagctgt atttcctgtt ttccaaaaaa
      661 agcccttgat caatgacttg ctctggtccg gaagttcaag cggttttgca tttacataat
      721 cgaaattctt atctggagta taattaaaaa ttgcacgtgc tttctgaaaa gcttctccac
      781 atggaattac actgcatcct agtttctttg aggcatcata atatgaccgt tttaatcggg
      841 caaacatttc attctggcta atcccatatt tctctaattc accggcatcg caccgataag
      901 cccaagtttg atgaattgcc atttgcgcct gaggtgcaaa tttttcaacc aagtcataaa
      961 gtttttctat atatggatag aatgtttccg ttttccagct caagtcactt acctgctgta
     1021 gagtaacaaa gttccacttc tcacttacga gagcctcttt aagtgtagca ctgcaggttt
     1081 cttttcctgt catataaaag ttgtatggct ttacatttgg aagtaaatcg cattgttcca
     1141 ccagattcca atgtttttcc aaagagcagc cgccaatatt agcctttcct attacaagcc
     1201 caacatcctt acctgctgcc gctgcaatct cttctaagta tcttgttgca tcatcggcaa
     1261 aactatttcc tatagtaagt gcctttacca ttttaatatt ccccttttta tagttattta
     1321 tttcataata attggattcc atgatataat tttcgcgaaa attccacccg aactccaaag
     1381 taaagctgtt aaaactaata atatgtattt ttgtgttatg catttactca gccaaatctc
     1441 cgttccttaa tctctatata gtcgtcttcc aaagcaccat gaaatgcagg tacagcatag
     1501 cttcccatat aaccgcccat aggaccggta tggtcagtac caccagtctt atataactta
     1561 agctgctccg ccttccggcg aaacatcttt tcatcttcct cgtccaacat ggggtgacac
     1621 acctcgatac cttgtatacc tatttttaca agcttgtcta catattcata ctggtttttc
     1681 ggatgtgcca gcactgcaat gccccctgct ttcctaatat acttaactgc ttcttctaca
     1741 cttgcctcct ttataggaag agcataagga gaatcataag agaaatttac cttatgtatt
     1801 tcctcccagt cttccttatg tataattccc ttatctacca tcgcacgaca tacctggtca
     1861 ttgcaaaacc attcgttctc cggatttcta tctattacct cttgccaagt tatacccttc
     1921 agtgtaccac gagaaagccc cgcttcaagg agttcccttg tatggtcatt acgatattta
     1981 catagacgtt ttacaaatgt tatcaactct atattgtcta catcaaaatc atatcctaga
     2041 atatgaaacc ccacccccca ctgtcggcag gtaaactcaa caccggacat actcttaata
     2101 cctatctttt cagcaagccg tgtaagttca cgtactccac taataacatc atgatcactt
     2161 agtacaacac cgccatagcc taatgacttt gctagaattg caagctggta tggagtaaat
     2221 tgcccatctg aatgagttga atgcagatgc atatttgcaa aatatggtgt aaaactcctg
     2281 aaatttttat tctcatttaa ttccatgatc catattccct tctatcatta tgttttgtat
     2341 ctttatacgg ttagatcgaa tctttttact acttttgcat aaaacgtttc catcttgtaa
     2401 acataatttc ttcaaatact tccctatgta tttcacaatg tatcatgtta tagacaacca
     2461 tatttgcttt aatattatct tcgttatcac ctatagctgt aaaatttttt actaatcttt
     2521 ctatcatatt ggttttgctg caatactctc cccatttatc tttagcaaaa ccgggatcct
     2581 ctgagagctc cctggaacgc ctatagaaag cgtctaatat ttccttacct tcttcatcat
     2641 tagaaagacg cattccatta tcatgccttg ttaatggaat ataatcaacg gttacttttt
     2701 tgtcatcaga tatccttatg ttaactaata tggatgtttg ccattcttcc gagtcatcct
     2761 ccccatatat aaacataaaa ttaccctgac catacactat tgtgccaccc ttataatctt
     2821 cataacagcc aatacagtga gtatgctgag taattatcaa atctgctcct ttttctataa
     2881 acttccggca aactttttgt gtattgggac taggatagcg atattcttcc ctgccaccgt
     2941 gataaagaac aataacataa tcacaaactt tttttagttc tgatacatga tcaaaagata
     3001 caagcggttc gtaaggattg gctcctgctg aattatcatt tgctattgta aactcatgct
     3061 cagcgcatgt atatatacca atcttaagtc catttttatg caaaataaat ggttttttag
     3121 cttcttctaa gtttttacct gcaccacaat ggttaatccc ctctttatca agcacttcca
     3181 atgtcttaaa aagagcttgt tcatcatgat caagaatatg attatttgca agacacagta
     3241 aagaaggttt gatggctgat attcccttaa ctgaagctgt tggtgctatt aaattgggac
     3301 cgcacttcgg aataggcttt ttaacatcgc atagcggagt ttccagatta aatattctaa
     3361 gatcactttt atctaacaca tcaagtatac ctcttccaaa aagcgcttca acatcgccat
     3421 ttataaatag ctccatatta aattcagtag gcacaatatc tgcacctata atgatatttg
     3481 tcatttaata atttcttcct ttcatattat ttttttatat agctattctt acataactat
     3541 aaaagttcat ctaatgcttc taaaagcgtt cgtctaactt cattgaaatt ctcagcagac
     3601 ggattatatt catcaaaggt tgtacaaacc cgagctatta tttcatcggc tttatttctg
     3661 tcccgtaaac ctagttgata tagtagttca aaatcctccg cccctgctct ttgcagatgt
     3721 gcgcgaatac tataccatgg tttgtcgtta ccggggtaga caatgttgct atttccgggc
     3781 ggcaacagct cttcaggttt tcctctattt aagcatgtat actcgaaagg ttcgtcacta
     3841 tatacattat atccccaatg caaaaaaccc ggtgcattat atttatagca catccacatt
     3901 ggaagacggc ttgccatcaa agaaagatca ataacgcggt tcatggtttt acctgccgga
     3961 tagccacagg tataaatcca tatttcttca ccccatgctt gcaggagctc atgttccgct
     4021 ttatgctgtt cataaaagct ttgctttact acccatatat ctactgctcc tcccagctct
     4081 gttgactcaa caggatcatt aattacaaca cccggcatac atttacggca gattcccgat
     4141 aaaatcctgt aatgctcact attgggaaaa ttcggctcat ccaccagaca ttgcatgtac
     4201 ttgttttccc agccattctc caaaattata ctccatagct ttgtaaaata aattttaagc
     4261 tgccggtacg cttcacggct tgcaacttca atgtccatat tccaaagcag atacagttcg
     4321 gttgcatccc attttttcca atgggataca aaaccaccat atatataagt gaatccttta
     4381 tcaatggcca ttttaccttc tttaatcatc tcactaaaat cgaagtcaac aacatttcca
     4441 ttctcatcta aaattggttt tccgcttgaa agcattaaat ggctactacg catatccaac
     4501 tgattatcca tatagtgtcc aattacatcc caaaatttct cgcttccata ctctaaattg
     4561 tgcgcttttg atacatgatc taaaaagagc caattcacca tactgaaacg acaattttca
     4621 aggccaggta caactgcctt gtacactctt agcttaatat taatcttacc ttcacaacca
     4681 ttgaaaataa gttttaatac agattcatat tcacccggct caagcttgct taatacacta
     4741 attctgaaat aaaaggcagc cctgcctttt ttcaacatac catcgtctat atcacgtgtt
     4801 gcatcatata cataaaacgg tgcttgtcgg gtcacaaagt cctttacact atcataatcc
     4861 aaggtcgtat ataactttac accactattt tcttctaccc taacgggaat tagttgatac
     4921 ggaattagtg ttaaaccttc aacgtcctct ccgctccatt caattgagat aggggtatcg
     4981 tcatctactt gtaaattagt aagaacctga aaactaatat ttgcatttct tgctgtatcc
     5041 aagcaaatac tatctgatag ttctgttatt tcgctgtcag gataaaccca atcattttca
     5101 ctgaatattt gcagtttcat tataataaca caacctttcg tttattttct attatcactc
     5161 gtgtctccaa tatatgacta tagaactcat tatgtaagtt tttcgcgtct cctctaaagt
     5221 ttaataatag aataaccagc ttttttaagt tcatcaatca tccatggaat atcaaattct
     5281 ttcagaactg aaaaaatatc atatttgcaa ttatattgat aaaatttggt ttttacaatt
     5341 tcttcaaaag aaggcatcgg atctttaagg taaatacttt gaacatcttt aactaatata
     5401 gcttcaacaa gagcataaaa agaacataca cctttaccgg ctatcccggg tattatgcca
     5461 ttgagcctcc gtttaacata atcgtgagct ctaatcacat cgtatattct taatgcagca
     5521 agggatgtcc ccatcatcat agcatccgaa ttgagtttgt actctgtgcc ataaatatca
     5581 ttataacctc tgggattaac tttgcgactt tttgcagcac ccattcccct tgggtcgaat
     5641 acaaaaacac ttccattatc aagaaggtgg ttaatcaact ctttttcatt ttctaaagca
     5701 tttgtaccct cttccagcag aagaattgtg caattttttt cagaaagttc tctgccttca
     5761 ggagttatgt atattccatc aacagcaata tcttcttcag aaaaaaagaa tattttttca
     5821 tatcttcttc cgtcaataac tttgtctgat acaacccggt catatataat ttcttctctt
     5881 tcatctaagg gctgtgggaa attcattact ttaattactc tttcctttat aaattccaag
     5941 ccaccttcag caccgacttc gtttttatag cggttattgc taaagtattc aaggttaaga
     6001 tcgaaaactg tctttgcatt ttcgtagtct gttaatattt gcccgctttt tgtacacaaa
     6061 agattgttat ccttttcagt aggtatattc ttgcttgtta taaaatccgg tttttgattt
     6121 ttaagatgtt ttctaaagaa attaactgta gattgcctta aaacatcgct atatccgtgg
     6181 actgaatcgg aaatacacat ctctatcagg tcttttttcc catatatttc ataaattttc
     6241 tttgctctct caaaggattt aacagcacct tctatgcaga aaaaatcata tcttgctgaa
     6301 tttataagca ccggtttagg tgcaaatgca gtaatgaaat cgtcatgatt aaggccataa
     6361 gcaatgctac caaaaatatt ttgttcatta tcatgggcct gccctgtttt catataattt
     6421 tcacgagaat taatataaca gctgggagct gccgctttaa gcctttcatc cacaagcata
     6481 atgtatgaag tctgtgtacc tccgcccgag ttgccgcaca ttccaaggcg ttcggggtca
     6541 acagccggat gagatgaaag gtagtccaat cctctaataa ggtcatgtat aaaatatcgt
     6601 gcaatattac tacctgttag ttcgcactgg agtccggcat atgaatgctc acgtgtacct
     6661 ataccaatag aacttgtgcc tgtttcacta tcataatact gtaccctttc tccctgacca
     6721 agaggatcta ctgcaagtac cacaaacccg tttatcacca aatcaataca taccctttga
     6781 taggcaggct ctgctttacc attatcggaa tgaccacagg caaacaggat acccggatgt
     6841 ttttctccag aacatgtttc aggaagataa agatttgagg taacataaaa tcctggatag
     6901 ctttcataaa ttatcttctc aatagtatag ccatcatact ttaaagagcc tgtacaaatt
     6961 gtattaagtg gtgttttaac gttttctaaa cctccaattg aagataaaaa gaaatctctg
     7021 gtctttcgcc tacaagcttc aaattgttga attgtatgta taccaagctt tttatttgat
     7081 tctgcttcaa aatattgctt tgcaagcttt tttagataat ctggcagttg acattctata
     7141 tcataataag aatctatgtt tgctgaaaac acggacatct ccccttaata atatttgtta
     7201 acaatgtact aatttacctg gataatacca ttttattgga ataatatcta ttgttcaata
     7261 ttcaaaaggt gcattttaat atacccctca taactaaatt gctttagcag ataagtatta
     7321 tatatcaaat gtaagctagg atgtatggaa attatttata ctatttatgt aatttttgag
     7381 ttacaaccat gctttttcca tatagaaaag catataaatt aacctggaag cttccactct
     7441 gtttgcagca tgcattactt aacaatagaa ccagtcgtca tcaatctgca tcatctctgg
     7501 caacccataa taaacctttc gccggagttt gaatgttgaa tatgcctcaa atgcttcaga
     7561 acagatatta tgcattttat agttactaac accaatatat tgatgtaact tgataataaa
     7621 aggagttcac caaagatgaa cccccttttt ttagctcatt gtcagtttat gattatttac
     7681 caacaaatat ttttgtaagt tcatcatgac gtccctgcca ccatgccctg gattcaacag
     7741 cacctgcttc attcattttt ttgatggttt cagcttttaa ctgtttgaat tcatcttcac
     7801 tttttgccat aacgattttg gggatagctt ttaatgccat atcattaagt ctcacatcaa
     7861 tacgagctat atcttccggt gcggtgccca tgcctgttac aacacgtaaa tcaaacacat
     7921 cattatcatg tgtaaaaata gtaccttcat ctattttctt tgcaaatact cccattgggt
     7981 attcagcgcc atagtaactg gagaagtcgg tatcaacagg cttattagac aatttgtaaa
     8041 tatccgggct catgaataag tcaacaagcc caccatcagg acaaatatcg gccattcctg
     8101 ctgctgttat agtttgcagt gttgattgaa aatgtccaaa tccaatacta ttaaattctt
     8161 cttttgattc tcccgcacga tccagataca atttaactgt tttatcagtc aaagcaggct
     8221 tcccatcaat aatatcccaa tgttcaccct taatcccgct atatgccagt cttgcgccat
     8281 cattatcaga aagcagatta atgactttca ttacatgttt tggactttta catgttttag
     8341 ggatagttat aaagcatgaa ttccatccgc ctttgtaaac tgagtctgcc caataagttg
     8401 tcccttcaac aggaattacc tgaaaacctt taatagattt tggatcattt tccagcattc
     8461 ttttttcaaa tgtgtcacag taattatcaa aaaccggagt aaggatttgt cccatatcta
     8521 ctttggctct gaaatctgca ctcttttgtg taaagctatc agggtccaaa attcctaaac
     8581 gataggcttt attatatagt tcagcagctt gccaatatgg actgttttca tcagtaaagt
     8641 tgtaaaccat tttaccatct gagatatgtg ttactatatg atcatttgaa tgataatatc
     8701 cataggtata attaaaacga tgagaaaagg aaaatatacg gtcagcccag aaactaattc
     8761 cataaaccgg gttcccttcc tctgttttag gatgcgcttc taccatcttc accagtaaat
     8821 caagataatc gtcaatacct ttgatttcgg ggcatccaag ctccttatag taatcccagc
     8881 gtgttaaata aattgaatga tatactgacg caggcctccc ctcacttcca gcatgacatg
     8941 gaagcacata aacttcaccg gaaccatcac tcaaaaaatc tttagccatc tttaaacgtt
     9001 cgggaaaata ctttttcaca tttggtccat acttgtcaat caactctgta agcggtaata
     9061 taagattacc ttgaattgct ggtaaaatgt ctgtctgact acttattacc atacagtcgg
     9121 tagtatcacc gccggcaaaa ataacctgtt ttttctcttt gtcagcaaat gaatacttga
     9181 tgcgaacacc tgttaattct tcgattttct tcagaatagc agtgtcttca gttccggagc
     9241 cgggccaacc gccgacatac ataaaatcca actctatcac ttcttgaggc tcggtttttc
     9301 ctccatccgg ctcttttgta ccttcagacg aattgctgtc attacctttt ttgctggtac
     9361 aacctgaaaa ggcagaaaag acaagcaata cacacatgat gattacaatt gttttcttta
     9421 attttttcat attatttctc ctttcattat gtatatttga tttattcata catgcttaaa
     9481 ctcctaaaac aagaatttta agcaatatag tccttaatta tggaattgac tatcctttaa
     9541 tagcgcctat catcatgccc tttacaaaat aacgctgcat aaaaggatat accattaata
     9601 taggaattgt aacaacagct gtaattgtca ttctaactgt agccggcgta attttcatag
     9661 catcttctga aagaataggg tttattttta gtgcgtccgc aacactttgt gctttctgat
     9721 tatataagta caaaacgtat tgaagggtat ataatttgtc attagtaata taaatatgtg
     9781 agtcaaacca gtaattccat tcagcaacca gcgaaaatac tgcgatcgtc gcaagaattg
     9841 gctttgacaa tggcataaca atacgtgccc agcatgtcag gatacctgcg ccctccaaca
     9901 tagctgattc ctcaagggat tccggaatct gctcaaaaaa agtttttgtt aaaacaacat
     9961 agtatgctga tatggctcca ggtataatat aaacaaaaaa tgtattgcgc agtccatatg
    10021 ctctcaaagt caaataagta ggaataagac ctccgcctac atacatggtc gctactacat
    10081 aacgatatat aaattttcgg aaatacatct ctttctttgt tacaagataa cctaaaaagc
    10141 ttgaacaacc tatagttata actgcagcca atatggtcct gaatacggaa actacagttg
    10201 cgagaggaat ttcctcaaga tgtataacag cccagtagtt ttttaatgta agcccacgcg
    10261 gcagtaaagc tacgcctttt gtggcttcta caggatcact aagtgaatat atcagcatat
    10321 agtaaaatgg atagaagcaa agaaacccaa atactgttaa tacaacaaag ttaattatat
    10381 caaatacaaa agaactaatg gaaacatttt ttttcatatt agcgattccc ctcttacatg
    10441 ttttgccagg ctgtttacag aaaatagtaa agttaagctg actgccgatt tgattattcc
    10501 tacagcagtt gcaaatgagt aatcttgtgt tcctattcct atacggtaaa tatacatttc
    10561 caaaacttca agtttttctg caaccattac attgttaaac gcaaaatatt tttcaaatcc
    10621 cgcagataag atattactta cttgaagaag gagcagtaca ataaaagttg gcatgactcc
    10681 tggtatcaca atatgccaaa tgcaacgaaa cctccctgcc ccatctattt tagctgcttc
    10741 ataaagcgtt tgctcaatac ttgatatagc tgcaagatat attatggaat cccaaccaag
    10801 actcttccag attccgaggt atagcataaa tagccatgct ttatctgcat ttgctaaaag
    10861 attaaattgc tttccagagc ccacatttaa tagcagatta ttaacaagcc cattgctcga
    10921 aaacaatcca aaacaaagag aataaactat tacccagctt acaaaatgag gtagcgtaac
    10981 tgtagtctgt aagacctttc taaacttgga tgaacgaagt tctgttaata aaattgcaaa
    11041 tattaccggt aaaggcgatg taagtaaaga tagcccactc attaccaaag tattaattaa
    11101 tgcatttata acatcttcct taaaaaatcc tatcagtttg aaatacttta atccaacaaa
    11161 ttgcatttta tcaaaagata aaccgggttt gtaattaaag aaagcaagcg accaacccaa
    11221 aaggggcaca tatctaaaca taaatacaag cacaacaaat ggaagtgcta ataaaaaaag
    11281 ctttaaacca tacggattta tcctctttac ttcttttttg tttttcttac atagatgtga
    11341 attcatatat accaaccttc attatgtatt gttttcatat catatataat ataaatcatc
    11401 ctgtaactaa attatataat ggtgtatctg gatagtaaat aacaagtatc tacaatttgt
    11461 tgcctaataa tacgattggt ttgttttcac aaataactat atcgtacaaa tatacaatat
    11521 atcttattat tttaagataa taggtaatgt gagaactaca cagagtcccc cattctcagc
    11581 atgctccaat ctaatgccat acggtgtacc ataatgaaga aatatacgac ggcttgt
//
LOCUS       DAOSDD010000139        11592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2138_length_11592_cov_5.063968, whole genome shotgun sequence.
ACCESSION   DAOSDD010000139 DAOSDD010000000
VERSION     DAOSDD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_11592_cov_5.063968"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PK733_12275"
     CDS             complement(<1..730)
                     /locus_tag="PK733_12275"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11353.1"
                     /translation="MGETIINSVKKALNILDILAFEDFENKGIGLSDLSKMTGLKPNT
                     LHNILRTMVICGYVQQYENLKYAAGPKCRSIGLLNRFAPGLPLCNKIEKLLSALSKKI
                     GEAVVFAVLVEGNRIPIVICDNDNVIKVDYRKLEQKNIYSLVTGRVLAAFADENGLKL
                     IKEKWGYPGKEWKGIDNDTDLNEAFRVIKENGVAKELDRYSLFSVAVPALGDNGEIAS
                     IGCYAPIFRCDDNKQLQILNELKKA"
     gene            complement(935..3496)
                     /locus_tag="PK733_12280"
     CDS             complement(935..3496)
                     /locus_tag="PK733_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11354.1"
                     /translation="MDRLLTEKVKAEVLNNGMDLVGFGPVERWENAPYLLSPKAILPE
                     SETVIVAGIHITDAWTEMGGEPDPQTLGPGGWMDQNSLLDRIAYRVVRLLNDYGYRAI
                     GVASSNIWRYRKMDGLDSWFAPDLSHIHAATAAGLAQIGWHGISITPEFGPRVRFISI
                     VTDARLVPTPMYDGPNLCDLCMECVKACPSAALKKDFDGNPHEVIIDGKKFRYANKNI
                     WRCAWAEHFNLDLSSKTLEKDHIDENDIILEKVNHPDKGHERGVCQKVCIPPHLRTDK
                     DSIGRSDKKISLNRINRRYPDSMPTLRKMRDDIIAKAVELGVEIAGVSRLDAEIKAGK
                     AVLSQAPGMNTVMAFALSIPKEAKSRRNYSSYVSEVYDYAVGRKMHQILLRIARFIED
                     YGYYAASYTGMVPVKGINLKDKISNSYCESNYHDVQVPYIAYELAEMAGIGVINESFL
                     TPEFGEDVIVGAVVTDANLDELKNNLNNEIDQALNQDSKQDLKQNPGQNIKHDTGHDA
                     ELNEKYINKPYISSRMLNKPGALRSVIEGIARQNLVSLFGVASAQVFDSIVPTLKENI
                     NEDELGYGVIDKNRRPHGKWVSKVIKENVRINNPQDYIDGAKSVIVLGMNFPNELIEN
                     SGLDTSKQIGTYAYYTYQTIFELRFAALEVANYLNTLGYKTLITENMLGVGSKVDNPR
                     GLIPDFRCNALEAVAAGLGEIGRNGGLLTPEYGAHQRQIVIITDAELTINEPYNGSKI
                     CTECGNCSLKCPMNAYEERYFSINIGNKEVEYPLISRHRCDWAKRYSLNKDEGPALIG
                     NKTHAEPPHAGELNIEDIAEACSLKDEIMKHRTVILEPCIRYCTAHSIKQHKDNK"
     gene            3855..4688
                     /locus_tag="PK733_12285"
     CDS             3855..4688
                     /locus_tag="PK733_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11355.1"
                     /translation="MKYLYKNNSYSDCSYSNYFRKHCIKNNRSLYILISYLIIVVILI
                     TGCGDSNKHKTEDDQLAVLKQELESKNSEIESLNKQLKSMQEELDIKNDELNKTRFEL
                     ESFHESKEQKNEGSENQSDVYDNHRKTVSLADIKLGYTMEQVSGVLGKNYKESEWEHF
                     GDIKDKMWTYDNGVIIRLNRDMVYSIELTNAGYRTSFDVMVGDSALESINKCKKVFKE
                     FVSIHSGDNKPNLGWFYTDNDEMIVLYFNKENNRFNDGVELSEKTKVEKIELLYVSSF
                     D"
     gene            complement(4860..7103)
                     /locus_tag="PK733_12290"
     CDS             complement(4860..7103)
                     /locus_tag="PK733_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11356.1"
                     /translation="MRCSNIHTVFKNSMFLKLLAGFLLIILLTSTFYLLSYNFFINNI
                     ELEIVNNASHNIDFTTAKLDEKLSQLKNILLNFSLEEVFSPLKVGRQSLYTQKQILDN
                     FIEKYKTPNENVLKYVKAIFILPEDSYKNIITTDTTINSDRFFEAFYKSDTYTKEFWQ
                     IEMTKEFNFKYYPTSKFLDYRNYKPKNPLTHTLMPLAFKNTFKSNFIIVALIDIGLLY
                     DSFNKFTNDDFCIYNSDKVLLYPSVQNLDFSNTDINNSTVYSKTKNGYLFSRDSVEDN
                     LIYSTFLSNSKLKQQLFKTNTVFRLITATSIFVSILISIYVVKTFNDPVKQIAEIIKL
                     SGNKSNNDVIGLNNIRDDVKQIIEENEEKDSMLEAYFYQSKLKGNYSPFNEIKKHFII
                     NNNNALICFTIHYKESCYNQLFEETGKGIFFLKECISLYLNSYFKDSITFQIENNKVI
                     SIFNVPEDILDISTIVNEIANKLKNEEIYVFFTITYSKVCSDTSQLHSTYNKLFEITK
                     YRKLINCTQILSEDSIKSTNNIYYLSSEQTDQFKNLLINCRHKECLEFVDTTLQYNHK
                     KGVNNFNIQLLCTEIINCCIKCLTNLFYKLPSCFDVASIYSQFNSFTAINEYADSCKL
                     FTTQVINYILNNQKGSDYIIDFITDYIEKHFKEEIYLDLLAEKLNLTNNYISSYFKEK
                     TGTNLNFYINNFRIKKAVELLENSSLKIADISCKVGFSSDNTFRRIFKKHIGKTPDEY
                     RKNMILL"
     gene            7451..8989
                     /locus_tag="PK733_12295"
     CDS             7451..8989
                     /locus_tag="PK733_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11357.1"
                     /translation="MSKKFIILVLVLAMCFSLTACTITKKADTNKDGTNENQQDSSIT
                     AEIKIFPKDKVTISMLLPNVVSWPVQDDWFAIKAIEEATNVKLDIQKVDAQGDNYNTK
                     FSLLVAAGGLPDIVPLYSVTQGNIYAMQGAFINVENYMSKTPNLKKWIEENKDHTDTF
                     RASNGNLYAYPGMGSGIDNRRVWFYRKDIFEKHNLKLPETPVTPDGLYDILKKLKELY
                     PDSYPLGNRSHGIELMSPSWHTMERVYYDFDNNKFVFGPVEDEWKEFLMFMNKLIKEQ
                     LTPPDMFSITTTGWIDLLVQNKAFITVDYVGRMDSVGASGKEYNPNFEFGFMPPVNGV
                     AKYTAVEYGCTLVSSSSRKIDDVIKLMDWFYTDEAIELLTWGKEGKTYKIIDGKKQFI
                     ADSYHDFVKKYGLTTIGLRTVHKEDVIMLSYGKEAVKATLESPQYELRNNPVEYMQLT
                     EAENEIKSTIGADIGKYASESRAQFILGEKSFDEWDAYVKEIEKMGLAKLLDMYTKAY
                     ERYK"
     gene            9096..10064
                     /locus_tag="PK733_12300"
     CDS             9096..10064
                     /locus_tag="PK733_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11358.1"
                     /translation="MEIIKRELNIKRLLQPDKSRQRNDLLRKVIRSKYLLILLLPCII
                     YYILFAYLPMWGILIAFKNYQLFAGFLKSPWVGFKYFKLFFSSPDIFELLRNTFLIGL
                     YSLIWGFPFPLIFALILNEVKNLKFKKLVQTISYMPHFLSTVVVVGLIQLFLSPTYGF
                     VNQILQRLRCGTIVFLQKSSYFRTIYISSGVWQNLGWNAIIYIAALTNIDPNLYEAAK
                     IDGANRFNCLIYITLPCISQTIITLFLLSTGNIVSVGFEKVFLLSNDAVLNVADVIQT
                     YVWRQGLQNANFSYGTAVGLFNSVINLLFLVMGNFLARRYSETSLW"
     gene            10079..10942
                     /locus_tag="PK733_12305"
     CDS             10079..10942
                     /locus_tag="PK733_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11359.1"
                     /translation="MKKNRFSVFDLLIYVILIVIIICILFPLLHVVAVSFSSAKYIFT
                     NDITIYPKGFNVEVYKWILRDPKIYNAYKNTLYYVSLGTVLSLIATSTGAYALSKKKL
                     VFRKKFMGAIILTMFFSGGMIPSFLTIKNLGIYNTTWAVVLPGLVSSWYLIVMKSFFE
                     AFPGEIEESGELDGLNDIGTFWYLVLPVSKPVLAAIGLFYAVGLWNSYYTPFIYLDSQ
                     NKFPLQLILRSLLVQGQGLEGRAGQSDQPVILESIKYATIIVSVVPIIIVYPFLQKYF
                     VTGVLVGSIKG"
     gene            10973..>11592
                     /locus_tag="PK733_12310"
     CDS             10973..>11592
                     /locus_tag="PK733_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11360.1"
                     /translation="MSMLKNGKVSFGVIGCRMGQEHIQSTITNPFVELKAICDINKDK
                     IQNIAKIYNINEKNCYTDYKELIKRDDIDAVVVATPDNLHEEHAVAALKAGKHVLCEK
                     PMALNTDECKRMIKASKEAGKKLMIGQVVRHSPGVVLAKKLIDNGEIGELFYVESEYA
                     HDYTKARGVGDWRVDPRRHPFIGGGCHAVDLLRWIAGNPYEVFAYA"
BASE COUNT         3816 a         1768 c         2033 g         3975 t
ORIGIN      
        1 ctgctttctt taattcgttt aatatctgca actgcttatt atcatcgcaa cgaaaaatgg
       61 gagcatagca gccaattgat gctatttcac cattgtctcc caacgctgga actgccacac
      121 taaaaagaga gtatctatca agttcctttg caaccccatt ttcttttata actctaaaag
      181 cttcgttaag atccgtatca ttatcaatgc ctttccactc tttaccggga taaccccatt
      241 tttctttaat tagcttcagc ccattttcgt ctgcaaacgc tgcaagtacc ctaccggtaa
      301 caagggaata gatatttttt tgctcgagtt tcctataatc tactttaata acattatcat
      361 tgtcacatat tacaataggt atacgattac cctcaactag tacagcaaaa acaacagctt
      421 ctccaatttt tttacttaat gctgagagaa gtttttctat cttattgcat aatggcagcc
      481 cgggagcaaa tctgttcaga agtccaatac tcctgcattt cggaccagct gcatacttta
      541 aattctcata ctgctgaaca tagccacaaa taaccatagt tctaagtata ttatgaaggg
      601 tattgggctt aagacctgtc atctttgaaa ggtcagaaag tcctataccc ttattctcaa
      661 aatcctcaaa ggctagaata tccagaatat tcagtgcctt ctttacagag tttattatag
      721 tttcacccaa atggttacac cccttaaata tattttatat ttgtttttat atttatattt
      781 atatttgtac ttgtatttgt atttttattt gaagcctttg gaaagcaaat tgtacctttt
      841 tcgacttatt atgctatgcc caaaggcctt aaaattatta tagaacctta ttattcgata
      901 tacaagggta acatgtagct tgaatagcta tatattattt attatctttg tgctgtttta
      961 tactgtgtgc tgtacaatac cttatgcaag gttccaatat gactgttctg tgcttcatta
     1021 tttcgtcctt caagctgcag gcttcagcaa tatcttcaat atttagttca cctgcgtgag
     1081 gcggttctgc atgagttttg ttcccgataa gagcagggcc ttcatcttta ttgagactgt
     1141 accttttagc ccagtcacat ctgtggcggc ttattagcgg atattctact tctttattgc
     1201 cgatattgat tgaaaaatat ctttcttcgt atgcattcat aggacatttt aatgaacagt
     1261 ttccgcattc tgtacatatt ttactgccat tgtaaggttc attaatagtt aattcagcat
     1321 cagtaattat tacaatctgt ctttgatgag ctccatattc aggggttaaa agacctccat
     1381 tcctgcctat ttctccaagg cctgcagcaa ctgcctccag tgcattgcat ctgaaatccg
     1441 gtataagacc tcttggatta tctacctttg aacctactcc cagcatattt tcagttatta
     1501 atgttttata gccaagtgta tttaggtaat ttgcgacttc caaagcagca aatcttagtt
     1561 caaagatagt ttgatatgtg taatacgcat aagttcctat ttgtttggaa gtatccagac
     1621 ctgaattttc aataagctca ttaggaaaat tcattcccag cacaattacg gacttagcgc
     1681 catcaatata atcttgggga ttgtttatcc tgacattctc tttaataact tttgataccc
     1741 atttgccgtg aggccttctg tttttatcta ttaccccata acccagttca tcttcattta
     1801 tattctcttt aagagtgggg acaatgctgt cgaatacttg agcagatgca actccaaaaa
     1861 gggataccag gttctggcgg gctatacctt caataacact tctcaaagcg ccaggtttat
     1921 ttaacatgcg agatgagata tagggcttgt ttatgtattt ttcattcagt tcagcatcat
     1981 gcccagtatc atgctttata ttctgtcctg gattctgctt taaatcctgt ttcgaatcct
     2041 gattcaaagc ctggtctatc tcgttgttta aattattctt cagttcgtca aggtttgcat
     2101 cggtaacaac tgctccaact attacatctt ctccaaactc aggagttaag aaactctcat
     2161 ttattacacc aatgcctgcc atttcagcca gttcataagc tatatagggt acttgaacgt
     2221 cgtgataatt agattcacaa taactattgc ttattttatc tttaagatta atgcctttaa
     2281 caggtaccat acctgtgtaa gatgcggcgt aatacccgta atcttcaata aagcgggcta
     2341 ttcttaagag aatttgatgc atttttctgc ctacagcata gtcatatact tcactcacat
     2401 aagaagaata atttcttctg ctttttgcct ccttggggat actgagtgca aaagccataa
     2461 ctgtattcat tccgggagct tgtgacagta cagccttgcc tgctttgatt tctgcatcaa
     2521 ggcgggaaac accagctatc tctactccaa gttctacagc ttttgctatt atgtcatctc
     2581 tcattttcct aagtgtaggc atactgtctg gatatcttct atttattcta tttagtgata
     2641 ttttcttatc tgaacgtcca atagagtctt tatcggttct gagatggggc ggtatgcata
     2701 ctttttggca gacacccctt tcatgtcctt tatccggatg atttaccttt tcaagaataa
     2761 tatcattttc atcaatatga tctttttcaa gggttttgga actgaggtca agattaaagt
     2821 gttcagccca ggcacagcgc cagatgtttt tattggcata tctgaacttc ttgccatcaa
     2881 taattacctc atgaggattt ccgtcaaagt ctttttttaa tgctgcagac ggacatgctt
     2941 tgacacattc catacaaaga tcacataggt tgggcccatc atacattggt gtaggtacaa
     3001 gacgggcatc agtaactatg gaaataaaac gtactctggg gccaaattca ggtgttatgg
     3061 aaatcccatg ccacccaatt tgtgctagac ctgctgctgt tgcagcatga atatgtgata
     3121 agtcgggtgc aaaccaactg tccaatccgt ccattttacg atatcgccag atattggaag
     3181 aggcaacccc aatagcccta taaccatagt catttaacag tctgactaca cgatatgcta
     3241 tcctgtcaag aagtgaattc tgatccatcc agccgcctgg tccaagagtt tgagggtcag
     3301 gctcaccacc catttcagtc caggcatcag taatatgtat accggcaaca ataacagttt
     3361 ctgattccgg aagtattgct ttagggctta aaagataagg agcattttcc caccgctcaa
     3421 caggaccaaa ccctaccagg tccataccat tgttaagtac ttcagcttta actttttctg
     3481 ttaataacct atccataatt taacattacc cccttagtta gaattaaatt tcgccgtgca
     3541 gccagcatga cgcttattca acattatgga attgagttca acaataatgt ataatagtat
     3601 tataataatt ctgaaatggt ttgtcaataa gttggctgat atagaaaatg tacctgaaaa
     3661 gaggtgtatt tctgaatttt gtatcaaagg aagaaaattt gcccagaaat gtaatattgg
     3721 attgtaatat taaaggtttt gatttaaaat tataaaaaac ccctgatttg cttattgttt
     3781 aacgaatgta tataatgagg tagcggaata ttttgtatgc tacagtaaat taaactgtaa
     3841 gggggaacat gttaatgaag tacctttata agaacaacag ttatagtgac tgcagttata
     3901 gcaactactt cagaaagcac tgtattaaaa ataacagaag tctttacata ttaatatcat
     3961 atttgattat tgtcgttatt cttataacag gctgcggtga tagcaataaa cataaaacag
     4021 aggacgatca gctggcagta ttaaaacagg agttggaaag taaaaactcc gaaattgaat
     4081 cattgaataa acagctaaaa tctatgcagg aagaattaga tattaagaat gatgaattaa
     4141 ataaaacaag gtttgaactg gaaagctttc atgaatcaaa ggaacaaaaa aatgaaggtt
     4201 ctgaaaatca atccgatgtt tatgataatc acaggaaaac tgtaagcctg gcagatataa
     4261 aactgggata tacaatggaa caggtatcag gagtattagg taaaaattat aaagaatctg
     4321 aatgggaaca ttttggtgat attaaggata aaatgtggac atatgataac ggcgtaataa
     4381 taagattgaa tagggacatg gtctattcaa tagaattaac aaatgccggc tataggacaa
     4441 gctttgatgt tatggttggt gatagtgctt tagaatctat taataaatgc aaaaaagtat
     4501 tcaaagaatt cgtaagcatc cacagcggcg ataataagcc aaatttagga tggttttata
     4561 cggataatga tgaaatgata gtattgtatt ttaataaaga aaacaatagg tttaatgatg
     4621 gtgtagaact atcggaaaaa accaaagtag aaaaaattga attactttat gtaagctcct
     4681 ttgattaagg atgaatagta gaatttcaaa ccttctaata acattatatt tcacatcata
     4741 tatgtagatg ttttcctgca aaattatcaa cgattctata atattgttga tttacacaat
     4801 aatatttgtt cgtattataa ataaagctaa gtaaaaaggc aaatgcgatt ctaagaatat
     4861 tatagcaaaa tcatattctt acggtattca tccggcgttt tacctatgtg ctttttaaaa
     4921 atccttctaa atgtattatc gctggagaac cccactttgc atgaaatatc tgcaattttt
     4981 aaagaggaat tctctaaaag ttccacagct ttctttatcc taaagttatt tatgtaaaag
     5041 ttcaagtttg taccggtttt ttccttgaaa taagatgaaa tataattgtt agttaaattt
     5101 aacttttcag ccagcagatc caggtatatt tcttctttaa aatgtttttc aatatagtct
     5161 gttataaagt caataatata gtcactgcct ttttggttat taagtatgta gtttataacc
     5221 tgagtagtga aaagtttgca tgaatcggca tattcattta tggcggtaaa agaattaaac
     5281 tgtgaataga tacttgctac atcaaagcat gatggcaatt tataaaacaa attagtcagg
     5341 cattttatac agcagttgat aatttcggta cataaaagct gtatattaaa attatttact
     5401 ccttttttat gattatactg taaagtagta tctacaaatt caaggcattc cttatgacgg
     5461 caattaatta ataagttctt aaattgatct gtttgttcag atgataaata gtatatatta
     5521 ttagtagatt ttatgctatc ttctgataaa atctgtgtgc agtttattag ctttctatat
     5581 ttagttattt caaaaagctt attatatgtt gaatgtaact gggaagtatc actacatact
     5641 ttactatatg taatagtaaa gaaaacataa atttcttcat ttttaagctt atttgctatt
     5701 tcattaacaa tagttgaaat atccagtata tcctccggta cgttgaaaat ggaaataact
     5761 ttattatttt ctatttgaaa ggtgatagaa tctttaaaat aactatttag atataaactt
     5821 atgcattctt tcagaaaaaa aataccttta ccggtttctt cgaataattg attatagcat
     5881 gattctttat aatgaattgt aaaacatatg agagcattat tattgttgat aataaaatgc
     5941 ttttttattt cattgaaagg agagtaatta cccttaagct tggattgata aaaataagct
     6001 tccagcatgg agtctttctc ttcattttct tcaataattt gttttacatc atcacgaata
     6061 ttatttaagc caattacatc attattgctt ttatttcctg ataatttaat aatttctgct
     6121 atttgcttaa cagggtcatt aaatgtttta accacataaa tagatattaa tatactaaca
     6181 aaaattgaag tagctgtaat caatctgaac actgtattag ttttaaataa ttgttgtttt
     6241 aatttggaat tggaaagaaa tgtagaataa atcaaattat cttcaactga gtctcgtgaa
     6301 aacagatacc cgtttttagt tttactatac acagtactgt tgttaatatc tgtattgcta
     6361 aaatctaaat tttgcacaga agggtaaagt aataccttat cagaattgta aatgcaaaag
     6421 tcatcgttag tgaatttatt aaatgagtca taaagcaagc cgatatctat tagtgcaact
     6481 attataaaat tcgatttgaa tgtattttta aaagctaatg gcattaatgt atgggttaga
     6541 ggatttttcg gtttgtaatt tctgtaatct aaaaatttgc ttgtaggata atacttgaaa
     6601 ttaaattcct ttgtcatttc tatttgccag aattcttttg tgtaggtatc acttttataa
     6661 aaagcttcga aaaatctatc tgagttaatt gtagtatcag ttgttataat gtttttatag
     6721 gaatcttcag gcaatataaa aatagcttta acatatttta aaacattttc gttgggtgtt
     6781 ttatatttct caataaaatt atccaaaatt tgcttttggg tataaagact ctgccttcca
     6841 acttttagcg gtgaaaaaac ttcttcaagg ctgaaattca aaagtatatt ttttagctgt
     6901 gaaagttttt catcaagctt ggcagtagta aagtctatgt tatgactggc attattgaca
     6961 atttcaagtt ctatattatt tataaaaaaa ttataggaga gcagataaaa agttgatgta
     7021 agtaatatta taagtaaaaa cccagcaagc aattttaaaa acatagaatt tttaaatacc
     7081 gtatggatat tagaacatct catctaaaaa cctccatgtt aaattaattc tgactttttg
     7141 ctcatataaa ttcagataaa gtttagtaca gttatagttt aatataatta tttgcagctt
     7201 tcaatatgat ttatttaata ttttgtttaa tgaacattta aatcagcctg ttgagcttat
     7261 tccagcaagt accattcatt ttctacaata acaatcaaat taactgattt tagtaatatt
     7321 gaactatttt attgtttttg caggttgaat cactcatctt attaaattat catatacttg
     7381 taaatgcaga tctgcaaatt tgttttatga aatttatgtt caaattccaa tgttaaggag
     7441 gaataggatt atgtcaaaga agtttattat cttagtttta gtgttggcta tgtgtttttc
     7501 tttaacagca tgtacaataa caaaaaaagc tgatactaat aaagatggta ctaatgaaaa
     7561 tcaacaagat agcagtatca cagcagaaat aaaaatattt ccaaaggata aggtaacgat
     7621 aagtatgttg cttcctaatg ttgtttcgtg gcctgtccaa gatgactggt ttgctataaa
     7681 ggcaattgaa gaagcaacca atgttaaact ggatattcaa aaagttgatg cacaaggcga
     7741 caattataac acaaaattta gtttgcttgt tgcagcaggt gggttgcctg atattgttcc
     7801 tctttattct gtcactcaag gaaacatata tgcaatgcag ggagcgttta tcaatgttga
     7861 aaattatatg agcaaaacac ctaacttaaa aaaatggatt gaggagaata aagaccatac
     7921 agatacattc agagcttcga atggcaattt atatgcttat cccggaatgg gttccggtat
     7981 tgacaacaga agggtatggt tttatagaaa agatatcttt gaaaaacaca acttgaaatt
     8041 accggaaaca ccagtaacac cagatggatt atatgatatt ttgaaaaaat taaaagaatt
     8101 gtatccagat tcttacccgc ttggaaatcg atcacatgga atagaactta tgagtccgtc
     8161 atggcataca atggagaggg tatattatga ttttgataat aacaaatttg tatttgggcc
     8221 tgtagaagac gaatggaaag aatttcttat gttcatgaat aagcttataa aagaacaatt
     8281 aacacctccc gatatgtttt caattactac aacaggatgg attgacttac tggttcaaaa
     8341 taaagctttc ataacagttg attatgtagg tagaatggac agtgtaggcg cttctggaaa
     8401 ggaatataac ccgaattttg aatttggatt tatgcctccg gttaatggag ttgcaaaata
     8461 tacagctgta gaatatggct gtactttagt atcatccagt tcaaggaaaa ttgatgatgt
     8521 aattaagttg atggactggt tctatacaga tgaagctata gaattgctca catggggtaa
     8581 agaaggaaaa acttataaga ttatagatgg gaagaagcaa ttcatagctg atagttatca
     8641 cgattttgta aaaaaatatg ggttaacaac aataggatta cgtactgtac ataaagaaga
     8701 tgttataatg ctaagttatg ggaaagaagc tgtaaaagca accctagagt caccacaata
     8761 tgaattaaga aataatcctg ttgaatatat gcaattaact gaagctgaaa atgaaataaa
     8821 atcaactatc ggagcagata taggtaagta tgccagcgaa agcagggcac aatttattct
     8881 tggagaaaaa agttttgatg aatgggatgc atatgttaag gaaatcgaaa aaatgggctt
     8941 agcaaaattg ttggatatgt ataccaaggc atatgaacgg tataaataaa atttaggttg
     9001 tggcttatac aattaaattg gggaattgtg tcagattatt tcgcatagtc tgacacgata
     9061 ctttgctgat ttgagagtga ggagtgattt tgttcgtgga aattataaag agagaactaa
     9121 atataaaaag actgttacaa ccagataaaa gcagacaaag gaatgatcta ttaagaaaag
     9181 ttataagaag caaatattta ttaatactat tactaccatg tataatatat tatatattat
     9241 ttgcttacct tcccatgtgg ggaatattga tagcatttaa aaactatcaa ttatttgcag
     9301 gatttctcaa aagcccctgg gtagggttta aatactttaa gttattcttt agctcaccgg
     9361 atatatttga attacttaga aatacattct tgattgggct atattcactt atctggggat
     9421 tcccatttcc attaatattt gctcttattc ttaatgaagt aaaaaatcta aagtttaaga
     9481 agttggtaca gactattagt tatatgccgc attttctttc tacggttgtt gtagttggat
     9541 tgattcaatt gtttttatca cctacatatg gttttgttaa tcaaatatta caaaggctca
     9601 ggtgtgggac aattgtgttt ttacagaagt catcatattt caggactata tatatatcat
     9661 caggtgtatg gcagaatttg gggtggaatg caataatata tattgctgca ctaacaaata
     9721 ttgatccaaa tttatatgag gcagcaaaaa ttgatggtgc aaataggttc aattgtttaa
     9781 tatatattac actgccttgt atttctcaaa caataatcac tttattccta ttgtctaccg
     9841 gtaatattgt gagtgtaggt ttcgaaaaag tgtttttgct ttcaaatgat gctgttctga
     9901 atgtagctga tgttattcag acctatgtat ggagacaagg actgcaaaat gcaaatttca
     9961 gctatggaac tgcggttggt ctattcaatt ctgtaattaa tttactattt ttagttatgg
    10021 gtaatttttt agcaagacga tattctgaaa caagtttgtg gtagtcgggg ggagaaatgt
    10081 gaaaaagaat aggttttctg tatttgattt actaatatat gtaattttaa tagtaataat
    10141 tatttgtatt ttatttcctt tgcttcatgt agtagcagta tcattctcaa gtgcaaagta
    10201 tatatttacg aatgatatta ctatttatcc aaaagggttc aatgttgaag tgtataaatg
    10261 gattttaaga gatccaaaaa tatataatgc ttataagaat acgttatact atgtaagttt
    10321 aggtacagtt ttatcactta tagctacatc tacaggagca tatgctttaa gcaagaaaaa
    10381 actggtgttc cgtaaaaaat ttatgggtgc aattatttta accatgtttt tttccggcgg
    10441 tatgattcct tcgtttctta caataaagaa tctgggtata tataatacta cctgggctgt
    10501 agtattgcca ggtcttgtga gttcatggta ccttattgtt atgaaatcat tttttgaagc
    10561 ttttcctggt gaaattgagg agtctggtga gctggatggt ttaaacgata ttggaacatt
    10621 ctggtatctg gtcttacctg tttccaagcc tgttttagct gcaatagggc tattttatgc
    10681 agtaggacta tggaatagct actacactcc atttatttat ttggattcac agaataaatt
    10741 ccctttacag ttaattctaa ggtctttact tgtgcaaggt caagggcttg aaggaagagc
    10801 gggtcaatct gatcagcctg taatactgga atcaattaaa tatgcgacca taatcgtttc
    10861 cgttgtacct attattatag tttacccttt ccttcaaaaa tatttcgtta ctggggtgtt
    10921 agtaggttca attaaaggtt gagcgtaaaa aaggtaaaag aggtgtttga aaatgtctat
    10981 gttaaaaaac gggaaagtta gttttggtgt tataggatgc agaatgggac aggaacacat
    11041 tcaaagtaca ataactaatc cgtttgtgga acttaaggcc atttgtgata taaacaagga
    11101 taaaatacaa aacatagcaa aaatttataa tattaatgaa aaaaattgtt atacagatta
    11161 taaagaacta attaaaagag atgatattga tgctgtagtg gttgccacac ctgataacct
    11221 tcatgaagaa catgctgttg cagcattgaa agcgggaaag catgttttat gtgaaaagcc
    11281 aatggcactt aatactgatg agtgtaaaag aatgattaaa gcttctaaag aagccgggaa
    11341 aaagcttatg attggtcagg ttgtaaggca tagtccagga gtagtgcttg caaaaaagct
    11401 aattgacaat ggagagattg gggaattgtt ctatgtagaa tcggagtatg cccatgacta
    11461 taccaaggct aggggggtag gagattggag agtagatcca agaagacatc cttttattgg
    11521 tggtggatgt catgctgtag atttattaag atggattgca ggaaatcctt atgaagtgtt
    11581 tgcatatgct aa
//
LOCUS       DAOSDD010000140        11289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2209_length_11289_cov_4.881698, whole genome shotgun sequence.
ACCESSION   DAOSDD010000140 DAOSDD010000000
VERSION     DAOSDD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_11289_cov_4.881698"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK733_12315"
     CDS             <1..335
                     /locus_tag="PK733_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOJ11361.1"
                     /translation="EGYIRCQRLTDDGYEVIEMPLPALITVVKEINEPRLPSLKGMMK
                     AKKAIVKVWTAEDINADTNLCGLKGSPTQVVKTFTPVHDIKSEILEGSPDEQAGRLAD
                     RLLNKVKV"
     gene            357..1559
                     /locus_tag="PK733_12320"
     CDS             357..1559
                     /locus_tag="PK733_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ11362.1"
                     /translation="MTSINIIKEKCVGCKLCISSCPYTAINIKDKKAEILDNCVLCGA
                     CVSSCKFGAIRFEKDEENKNADLSSYKGVWVFAEQKGGVPAGVVLELLGEGRKLADQL
                     QVPLSAVLLGDNVGQTAENLIYYGADEVYLLENPSLKIFNDEIYSNIIEKLINMYKPE
                     IVLMGATTYGRSLAPRVASQLNTGLTADCTQLAIDMDRKILLQTRPAFGGNLMATIIC
                     PNHRPQMSTVRPKVMKPMEPDYSRVGKIIKPEINVPDDHDIKIKVNEVVTTLNEAVSL
                     TEADIIVSGGRGLGDPKNFALVEELAKVLGGAVGASRATVDAGWVDYSHQVGQTGKTV
                     GPKVYFACGISGAVQHLAGMSSSDIIIAINKNPDAPIFKIATYGIVGDVLEVLPALIK
                     EFKCRLNV"
     gene            complement(1638..2273)
                     /locus_tag="PK733_12325"
     CDS             complement(1638..2273)
                     /locus_tag="PK733_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOJ11363.1"
                     /translation="MTITSITRNEKDKSVYDVHIDNAYSFSVSEEDYFRLNLYEKREI
                     SEEELKLIKKTVSFALAKTKALGYLSFKIRTAREVEDKLIKEGFSEDLTGNVVNDLKA
                     DGYINDMLYVRKYIYDRNKLKPKAKRMLAYELKKKGIDDDTIADGLTELKIDNILVAK
                     ELICKKFRGCDLSDVKIKHKVFQFLRYRGFSESEIKKAINEANNEMNEMND"
     gene            complement(2335..3387)
                     /gene="recA"
                     /locus_tag="PK733_12330"
     CDS             complement(2335..3387)
                     /gene="recA"
                     /locus_tag="PK733_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOJ11364.1"
                     /translation="MIEKKKALDMALSQIEKQFGKGAVMKLGENTHLNVEVIPTGALG
                     LDIALGVGGVPRGRVVEIFGPESSGKTTVALHIVAEAQKNGGEAAFIDAEHALDPVYA
                     RNLGVDIDNLIVSQPDTGEQALEIAEALVRSGAIDVIVIDSVAALVPKAEIDGEMGDS
                     HVGLQARLMSQAMRKLAGVISKSRTTAIFINQLREKVGIMFGNPETTPGGRALKFYST
                     VRLDVRRIESLKQGTEIIGNKTRVKVVKNKVAPPFKEAEFDIIYGEGISKEGNLLDVA
                     VNLDIVNKSGSWYSYNDQRIGQGRENAKQYFKENKDTAKEIENKIRENFKTAFAKSMG
                     IHTEETEKEEIPDFEE"
     gene            complement(3520..5106)
                     /gene="murJ"
                     /locus_tag="PK733_12335"
     CDS             complement(3520..5106)
                     /gene="murJ"
                     /locus_tag="PK733_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOJ11365.1"
                     /translation="MEKNRLTGAAAIVMSSMVVSRLTGFLRSTLIPNLMSKMESDALF
                     AAFKTTDLMYNLLVGGSIAAALIPVLSGYIVKNMEEEGWKAVGSFINTIFLIMVAISG
                     LGILFAPQVVSLTAPGYGMETTELTIRLTRILFPSVAFIMLAGLTNGVLNSYQRFAAA
                     AYGPSIYNLGSVVSILAFHKSGVEKVAFGIMISSVVYFLFQLSFAFRNLEYYRFKIHF
                     RHPGYKKIIKLAIPSLAASSIVQVNTIISQSYTSNFKGGSVTALTNANDIWQLPYGIF
                     AMGMGTALLPTLSEKVAVGDAESFKKIFIKGIKSVLLLTIPSGIGFIVLRGPVISAIF
                     KWSGNFGPDRILMTGNILMFYSLALFSQSVLAIVNRAFYAYNDTKTPLYSGIASIITN
                     AFFCYVFFYYTNLEAAGMALAYSIAGTVNALILLYILNKKAEGIKLRELILYILKILT
                     ASVIMGAILYLICFFTPIDFARPFTIRGKIVELVYLLFEIAIGVLCYFSIVLFMGIEE
                     AKYVFTLAISKLYKIIVKAK"
     gene            complement(5174..6421)
                     /locus_tag="PK733_12340"
     CDS             complement(5174..6421)
                     /locus_tag="PK733_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOJ11366.1"
                     /translation="MNAEILAVGTELLLGQIVNTNAQYISQRLPEVGVNVYYHDVVGD
                     NPKRLRESLSMALKRSDVVILTGGLGPTKDDLTKETVAQALNRKLVLDEESFDIMKSF
                     FARFNRQMTENNIKQAYLPEGSIIIKNKNGTAPGCIIEDGDKVVIMLPGPPSEMRPMF
                     EETVISYFRNRSDCKLVSKFVRIFGIGESAVEDKIMDLVDNQTNPTIAPYAKQGEVML
                     RITAKCENGEDSDALINPVIEEIKKRLGDTVYSADNKEMEEVVAELLLSSGLTISIAE
                     SCTGGLISKTLTDIAGISQVFMSGVVSYSNSAKINILGVRPITIEKYGAVSENTAVEM
                     AENIRNISGTDIGLSVTGIAGPGGATPEKPVGLVYVALASKDGVQIKELKLWGERSRI
                     RNVACLNALDMIRRYVLKQPELK"
     gene            complement(6647..7681)
                     /locus_tag="PK733_12345"
     CDS             complement(6647..7681)
                     /locus_tag="PK733_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11367.1"
                     /translation="MGNLRFGIIGCGLISNWHADAILQIEGTQLVGATDVNEKARAAF
                     ALKYNILAFNSVEELLASDDVDIVCICTPSGLHAPLAVKAANAGKHVIVEKPMAITLK
                     QADNIIEACEKNNIKTTVISQSRFTNSICKLKNAVDKGLLGRLVSGDAYMKFYRSREY
                     YDSGGWRGTWEMDGGGALMNQGIHSVDLIQYIMGPVKTIFAYTRTLARKIEVEDTAAA
                     VLEYENGALGVIQATTSIYPGLPRRIEVSGDKGTIIVDGHSITNWTIEGNEVPDGIEI
                     GKANRTSASDPAAFGIEGHIMQISDMVDAIKNNRNPLVDQYEGKKPVEIILAIYESSR
                     TGKTIEFSKY"
     gene            complement(7873..8754)
                     /locus_tag="PK733_12350"
     CDS             complement(7873..8754)
                     /locus_tag="PK733_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11368.1"
                     /translation="MKKIDIHAHTRMYVGIPRMGTNSTFATPEQLIEHYKKLGVEKAV
                     ILPGVGIEYSDHIQSNEEVIALTEKYPDWFYWFCNIDPRMGKNRPDTDLSYFLNYYKK
                     LGAKGVGEVCSNMYFDDPYMENLFYHCEKCNMPVLFHIGPQIGGCYGIVDEIGLYRLE
                     KELSKFPNLIFIGHSQPFWAEISSDVTENNRNEYPKGKVTEGRLVELMRKYPNLCGDL
                     SANSGYNAVSRDPEFGYAFIEEFKERIFFGLDICSPENKRELSSWLDKAYQNKKISED
                     AYVKVCRGNVLRLLGEI"
     gene            complement(8884..10605)
                     /locus_tag="PK733_12355"
     CDS             complement(8884..10605)
                     /locus_tag="PK733_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ11369.1"
                     /translation="MKSEHKYIKSLISKMTLDQKIGALLTLGFAGTIARPHIYDYITK
                     YHCGGLRLSPGSRHFGSYIDPRTGKTIVSIEDTRGYKQGISPPYVSVFEYKRILDELQ
                     GVAMSRPLGIPLHFSFDQEGGAGVNSRFTGVNFFPRSMGIRATGDSRFAYEAAKAVAR
                     QSKSVGFNWIHSPVLDINVQPNNPEIYTRAYSDRVNEVVEYSIEACRGFKEINVIATG
                     KHFPGRGDSSFDAHYDIPIIDTDKKTLMNRELLPYSELIKLNLLPSIMLAHTIYPAID
                     DNDVATVSKKVVTGLLREELGFGGVVTTDSMTMGGIAVKYGVANACALALEAGADLVL
                     MKAETELVDDTISTIRRFVENGRISTSELDKKVYRVLSLKYEYGLFTYGNNWAEKPED
                     TLEDRQIIDLSKLVARKSVLIAKDKNKLLPLPRNEEITVIEQINFTPNDIHWHPGILY
                     KNCTKYNRNARYLETAYTYDESDKDAIINTLKGCKTVVITNFYERGRASNNEFISELL
                     ANKSNKIVIITNTPYPLSIPKGSDCIIITFGTSPANVEVVAGVLFGGIIPEGEWPIEY
                     VPDVLNG"
     gene            complement(10658..>11289)
                     /locus_tag="PK733_12360"
     CDS             complement(10658..>11289)
                     /locus_tag="PK733_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11370.1"
                     /translation="IGIEEEIYTDDNTKLQTIIDEINKKNGIAIIAHPAWSLITHDDL
                     IDLHDYFGIEIWNHANDVKSLRGDASIYVDVVASKGVVKYLFATDDSHRYTNDAFGGY
                     IMVNSERLDKKSIFESIKAGNFYSSQGPEIRQITVDKDKIIAECSPVKKIVFLNDTFS
                     SGDRITASETGLISKGVYRIKETDTVVRVECIDENNKKAWSQFIKVNQI"
BASE COUNT         3373 a         2306 c         1744 g         3866 t
ORIGIN      
        1 aagaaggcta tataagatgc cagagattaa ctgatgatgg gtatgaggtg attgaaatgc
       61 cccttcctgc attaataaca gtagtgaaag aaataaatga accccggctt ccttcactta
      121 aaggtatgat gaaagcaaaa aaagctattg ttaaagtatg gactgcagaa gatataaatg
      181 cagatacaaa tttatgtgga ttaaaaggtt ctccaactca agttgtaaag acatttactc
      241 cagtccatga tataaagagc gaaatacttg agggttctcc ggatgaacag gcaggaaggc
      301 ttgctgatag attgcttaat aaggtaaaag tataattggt tatgatggag gtcagtatga
      361 caagcattaa tattataaaa gaaaaatgtg ttggctgcaa actttgcatt agttcatgcc
      421 catataccgc cataaatatt aaagataaaa aggctgaaat acttgataac tgtgttttgt
      481 gtggtgcatg cgtatcatca tgtaaatttg gggctatacg atttgagaag gatgaagaaa
      541 ataagaatgc agatttatca tcctacaaag gtgtatgggt gtttgccgag caaaaaggcg
      601 gtgtacctgc aggtgttgtt ctggaacttc tgggagaagg cagaaaactt gcggatcagc
      661 ttcaggttcc tctttcagca gttctattag gtgataatgt aggacaaaca gcagaaaatc
      721 tcatatacta tggtgctgat gaagtttatc tgttggaaaa tccttcacta aagattttta
      781 atgacgaaat atattccaat attattgaga agctgataaa tatgtataaa cctgagatcg
      841 tccttatggg tgccacgact tatgggcgtt ctttagctcc aagggtagcc tctcaattaa
      901 atacaggact tacagccgac tgtacccagt tggcaattga tatggataga aaaattcttc
      961 ttcaaacaag gcctgctttt ggtggtaatt taatggctac tattatatgt cccaaccata
     1021 ggccgcaaat gtctaccgta cgtcctaaag tcatgaagcc catggaacct gattattcaa
     1081 gagttggcaa aataattaaa ccggaaataa atgtgcctga tgatcatgac ataaaaatta
     1141 aagttaatga agttgtaact actcttaatg aagcagtaag ccttacagaa gcagatataa
     1201 ttgtttccgg aggaagaggc cttggagatc cgaaaaactt tgctttggta gaagaattgg
     1261 caaaagtact aggcggagct gtaggagcat cacgtgcaac agttgatgct ggatgggttg
     1321 actacagtca tcaggtaggt caaacaggta aaacagtagg tccaaaagtt tattttgcct
     1381 gcggaatttc cggggcagtt caacaccttg caggcatgtc ttcttctgat atcattattg
     1441 caattaacaa aaaccccgat gcacctatct ttaagattgc cacatacgga attgttggag
     1501 atgtccttga agtactgccg gcattgataa aggaatttaa atgtcgatta aatgtgtgag
     1561 ctggaaataa gaagctagga gtctgctcgg aaagtctgag ctatcaaagc ggactcctag
     1621 cttctggcat ctataactta atcattcatc tcattcatct cattattggc ttcattaatt
     1681 gcttttttta tttcgctctc gctaaaacct ctataacgta aaaactgaaa cactttatgt
     1741 ttaatcttaa catcggaaag gtcgcatcct ctgaatttct tacatattag ctcctttgcg
     1801 actaatatgt tatcaatttt taattccgtc aaaccgtcag cgattgtatc atcatctata
     1861 ccttttttct taagttcata tgccagcatt ctttttgctt tcggttttaa cttatttctg
     1921 tcatatatat acttgcgtac atacagcata tcattgatat agccatcagc tttaaggtca
     1981 tttactacat tacctgtcaa atcttcgcta aaaccttctt taatcaattt atcttctact
     2041 tctctggcag tccttatttt gaaagacaaa tagcctaatg cttttgtttt agctagtgca
     2101 aaacttaccg ttttctttat taatttaagt tcttcttcag atatttccct cttttcgtaa
     2161 aggttcagcc tgaagtagtc ttcttcagaa acagaaaagg aataagcatt atctatatga
     2221 acatcgtaaa cacttttgtc cttttcgttt ctggttatag aagtaattgt catttactca
     2281 cctttgaaat tttgaaatta atatttgtta agtttctgca gaataatcat ataattattc
     2341 ttcgaaatcg ggtatttctt ccttttctgt ctcttctgta tgaataccca tacttttagc
     2401 aaatgcggtt ttaaagtttt ctcttatctt gttttcaatt tctttcgctg tatctttatt
     2461 ttccttaaaa tattgttttg cattctctct tccctgaccg attctctggt cattatatga
     2521 ataccatgaa ccgctcttgt tgacaatatc cagatttact gctacatcca gtaaatttcc
     2581 ttctttggaa atcccctcac catatattat atcaaattct gcttccttaa aaggtggagc
     2641 tactttattt ttaaccactt ttactctggt tttgtttcct ataatctctg tcccctgctt
     2701 taaggactct attcttctta catcaagcct tacagtagag taaaatttaa gcgctctccc
     2761 acccggagta gtttcgggat ttccaaacat aattcctact ttttctctaa gctggtttat
     2821 aaaaatagcg gttgtcctgg atttgcttat aacacctgca agttttctca tagcctgaga
     2881 catcaatctc gcttgaagac ccacatgcga atcacccatt tccccatcaa tttctgcttt
     2941 tgggactaat gcagctactg agtctataac aatgacatca atggcaccac ttcttacaag
     3001 tgcttctgct atttcaagag cttgctcccc tgtgtcaggt tgagatacta taaggttatc
     3061 aatatctaca cccaggtttc tggcatatac agggtccaat gcgtgctctg catctataaa
     3121 agcagcctcc ccaccatttt tctgagcttc tgcaactata tggagagcaa ctgtagtttt
     3181 accagaggac tcgggaccaa atatttccac tactcttccc cggggaactc ctcctacgcc
     3241 taaagcaata tcaagcccca gagcacctgt aggtattact tccacattta aatgagtgtt
     3301 ctcaccaagc ttcataacag ctcccttacc gaactgtttt tctatctggc ttagagccat
     3361 atctagcgct ttcttctttt ctatcatttt gctaacctcc ctaatcattc tggaacggat
     3421 gttctaacaa tattataata tactgttttt gattcgtcaa tagtttttca aaaaaataac
     3481 aaaaatgttt atcaaagcaa ttttagaaat gatttagcct tacttggctt taactataat
     3541 tttatataat tttgatattg caagggtaaa aacatatttg gcttcttcta tccccataaa
     3601 caatacaata ctaaagtaac acaacacccc aatagctatt tcaaaaagaa gatataccaa
     3661 ttccactatc ttgcctctta ttgtaaaagg tcttgcaaaa tcaataggtg taaaaaagca
     3721 aatcaaataa agtattgcgc ccattattac agatgctgtc aatattttta atatatataa
     3781 tattaattct ctgagcttga ttccttcagc ttttttattc agaatataaa gcagtataag
     3841 tgcatttaca gtgcctgcta ttgagtaagc aagagccatg cccgctgcct caaggttggt
     3901 ataataaaaa aatacatagc aaaagaatgc atttgttatt attgaagcta tacctgaata
     3961 tagaggtgtt ttagtatcat tataagcata gaaagccctg ttaacaatag ccaaaactga
     4021 ttgcgaaaat aatgcaagag aatagaacat tagtatgttt cctgtcatta atatcctgtc
     4081 aggaccaaaa ttcccagacc acttaaatat agcacttatc actggtcccc tgagaacaat
     4141 gaatccaatt cctgatggaa tggttaggag aagtacggat tttattcctt ttatgaatat
     4201 ttttttaaaa gattcagcat caccaacagc aaccttctca gaaagagtag gcagcaaggc
     4261 tgtccccata cccatggcga atatcccata aggaagctgc catatgtcat tggcgtttgt
     4321 aagagctgta acactcccgc ctttaaaatt tgaggtatag gattgtgata ttattgtatt
     4381 aacctgtaca atggatgatg cggcaagtga tggtattgca agctttataa tctttttata
     4441 tcctgggtgc ctaaaatgta ttttaaatct gtagtactct aaattcctaa aagcaaaaga
     4501 aagctggaat aaaaaatata caacagaact tatcatgatt ccaaacgcta ctttttcaac
     4561 acctgattta tgaaaagcca gaatactcac tacacttccc aaattgtata ttgatggtcc
     4621 atatgctgca gcagcaaaac gctgataaga attaaggacc ccatttgtaa gtcctgccag
     4681 cattataaaa gcaacagatg ggaaaagtat tcttgtaagt cttattgtta attcagttgt
     4741 ttccatgcca taaccaggtg cagttaagct gacaacttgc ggtgcaaata atatgcctaa
     4801 tccacttata gctaccataa tcaaaaatat tgtgttaata aatgaaccta cagctttcca
     4861 accttcttct tccatgtttt ttacaatata ccctgataac acaggtataa gtgctgcagc
     4921 tattgagcct cctacaagca ggttatacat taaatctgtt gttttaaaag ctgcaaataa
     4981 tgcatctgat tccattttag acatcagatt aggtattagt gtagacctca gaaaacctgt
     5041 tagcctgctg acaaccatag atgacataac aatcgcagct gctccggtta atctattttt
     5101 ttcaatatcc tccaattata tcccccgttt tcttttgttc ttaagttata cttgttaaca
     5161 ttatctatat tgattacttt aattcaggtt gttttagcac atatcttctt atcatgtcaa
     5221 gagcattaag gcaagcgaca tttcttattc tgcttctttc gccccagagt tttagctctt
     5281 ttatctgaac tccatctttt gaggcaaggg caacatatac caatcctacc ggtttttcag
     5341 gagtagcacc accagggcct gcaattcctg taacagaaag tccaatatcc gtaccggaaa
     5401 tgttccttat attttctgcc atttctacgg cagtgttttc acttactgct ccatactttt
     5461 ctatagttat gggtcttaca cctaaaatat ttattttggc actattacta tatgatacta
     5521 ccccactcat aaacacttgg gaaatgccag ctatatctgt tagggttttt gaaatcaacc
     5581 cgccggtaca tgactcggca attgatatag tgagaccact tgacagtaat aattcagcaa
     5641 ccacttcctc catttccttg ttatctgctg aatatacagt atcacccaaa cgttttttta
     5701 tttcttcaat aacagggttt attaatgcat ccgaatcttc tccgttttca cattttgctg
     5761 ttatcctcag cataacttct ccctgctttg cataaggagc aatagtcgga tttgtctggt
     5821 tatcaaccag gtccattatt ttatcttcaa cagctgattc tccaatcccg aaaattctta
     5881 caaattttga aaccaacttg caatcagacc tgtttctaaa atatgagatt acagtttctt
     5941 cgaacatagg ccgcatttca gagggtggcc cgggaagcat tattactacc ttgtctccat
     6001 cttctataat acatcccgga gcagtaccat ttttattttt tattatgata ctgccctctg
     6061 gtaaatatgc ctgtttaata ttattctcag tcatctgcct gttaaatctg gcaaagaaag
     6121 atttcattat atcgaagctt tcttcatcaa gtacaagctt tctattcaaa gcctgagcca
     6181 ctgtttcttt tgtaaggtca tcttttgtag ggcctaaacc accggtcagt ataacgacat
     6241 cagacctttt taaggccata cttaaagatt ctcttaacct ttttggatta tcccctacaa
     6301 catcatgata atatacatta acccctactt cgggaagtct ctgagaaata tactgtgcat
     6361 ttgtatttac aatttgtcct agcaataatt cagtgccaac tgccaatatc tctgcattca
     6421 tacaaaactc catctcctgt ctatttttat aaggtaaggg gacactcctc tcttgagcaa
     6481 agaagtattc cctttacatt gaggaatgtc cccggaagaa tccttatctt ttgtatatat
     6541 tataccagta ttttcttcag ataatcaatc tgtttttggg aaagggacac atctttaatt
     6601 tatgaagaat catattccag aaactatcgt tgaagcatca ctattattaa tatttactaa
     6661 attctattgt tttacctgtt cttgatgact cataaattgc cagaataatt tcaacaggtt
     6721 ttttcccttc atattggtcg acaagagggt tcctgttatt ttttattgca tcaaccatat
     6781 cactgatttg cataatatgc ccttctattc caaatgcagc agggtcactg gcagaagttc
     6841 tatttgcttt cccaatttct atgccatctg ggacttcatt accctcgata gtccaatttg
     6901 taatagagtg tccgtcaact ataattgtac ctttatcacc acttacttct atcctccgag
     6961 gtaatccggg gtatatggat gtagttgcct gaattactcc taatgctcca ttttcatatt
     7021 ccaatacagc tgcagcagta tcttccactt caatttttcg tgcgagagtt cttgtataag
     7081 cgaatatggt ttttacaggt cccataatat actgaataag atctacacta tgaattcctt
     7141 gattcattaa ggcacctcct ccgtccattt cccatgttcc tctccaacca ccactgtcat
     7201 aatattcccg tgaacggtaa aacttcatat atgcatctcc ggataccagt cttcctaata
     7261 aacccttatc aacagcattt tttaacttac atatagaatt agtaaacctt gattgggata
     7321 taacggtcgt tttaatatta tttttttcgc atgcttcaat tatattatca gcctgcttca
     7381 aggttatagc cataggcttc tctacaataa catgcttgcc tgcattggca gcttttacag
     7441 ctaaaggtgc atgcaggcca cttggtgtac atatacatac tatatcgaca tcatcactgg
     7501 caagaagttc ttctacagaa ttaaaagcta gaatattata ttttaaagca aatgctgctc
     7561 ttgccttttc atttacatca gttgcaccga caagctgtgt cccttcaatt tgtaatatag
     7621 catcagcatg ccaattagat atcaatccac atccgataat tccaaatctt aaattaccca
     7681 ttgataattt acacctccga actgcttata atttaattga taatgcttga tgaaaatctg
     7741 tatggcacta tattgcaaca attatctgta ttatagataa taactaaata tttaaatttt
     7801 tttgacttgg caatgctcta tatataattg tatcttttaa ctgattcttc atgggatatt
     7861 ttgatttatt tactaaatct ctccaagtaa gcgaagcaca tttcccctgc ataccttcac
     7921 atatgcgtct tcagaaatct ttttattttg atatgcttta tcaagccatg aagataattc
     7981 ccttttattt tcaggcgaac agatatcaag gccgaaaaat atccgttctt taaattcttc
     8041 aataaaagca tatccgaatt cagggtctct gctaacagca ttgtaaccac tgttggcaga
     8101 taaatcgccg cataaatttg ggtattttct cattaactct acaagcctgc cttcggtgac
     8161 ctttcccttt ggatattcat ttctattatt ttctgttaca tctgaactaa tttcagccca
     8221 gaaaggttgc gaatgcccaa taaatatcaa atttggaaat ttgctcagct ctttttccaa
     8281 tctatataat ccaatctcat ctactattcc atagcatcca ccaatctgtg gtcctatatg
     8341 gaacaaaaca ggcatgttgc atttctcaca atgataaaag agattttcca tataagggtc
     8401 atcaaaatac atattgctgc atacttcgcc cacacccttt gcccccagct ttttgtagta
     8461 atttaaaaaa taggataaat ctgtgtcggg gcgattttta cccattcgtg gatcaatatt
     8521 acaaaaccaa tagaaccaat ctggatattt ttcagtaagt gctattactt cctcatttga
     8581 ttgaatatga tctgaatatt caataccgac acccggtaat ataacagctt tttcaacacc
     8641 aagtttctta tagtgttcta taagctgttc aggtgtagca aatgtagagt ttgttcccat
     8701 tctgggaata ccaacataca ttcttgtatg tgcatgaata tcaatttttt tcataatgtt
     8761 gcctcctaaa ttttttcata agttagttaa cactatcact tttcaatacg caatgctagt
     8821 cccaacattt accaattaaa ttttcatatg cttgttaaca atatctcttc tctgccatac
     8881 ttattatccg tttaaaacat ccggaacata ttcaataggc cattcacctt ccggtatgat
     8941 ccccccaaat aatacacctg ccaccacttc aacatttgca ggtgaagttc caaaagttat
     9001 aataatacaa tctgacccct tgggaatact caatggatat ggcgtatttg taataataac
     9061 tattttgttt gatttatttg ctaacaattc acttataaat tcattatttg atgctcttcc
     9121 tctttcataa aagtttgtaa taacaacggt tttacaaccc tttagcgtat ttattattgc
     9181 atctttatca ctttcgtcat atgtgtatgc tgtttctaga tatctagcat tacgattgta
     9241 ttttgtgcaa tttttataaa gtatccctgg atgccagtgt atgtcattag gagtaaaatt
     9301 aatctgttca ataacagtga tctcctcatt tcttggaaga ggaagtaatt tattcttatc
     9361 tttagctata agaaccgatt ttcttgctac cagctttgag aggtcaataa tttgcctatc
     9421 ctctaaagta tcttcaggtt tttctgccca gttgttacca taggtgaaaa gtccatattc
     9481 atatttaagg cttaacactc tatatacttt tttatcaagt tcacttgtgc ttattctacc
     9541 attttctacg aatctcctaa ttgtactaat agtatcatct accaattcag tttcagcctt
     9601 catcaatacc aaatctgctc ccgcttcgag agcaagggca caggcatttg ccacaccata
     9661 ttttacagct attccaccca tagtcatact gtcggtggta actacacctc caaaaccaag
     9721 ctcttctctc aaaagcccgg ttacgacttt ttttgataca gtagctacat cattatcatc
     9781 tattgccgga taaattgtat gagcaagcat aatggaaggt aataaattta gttttatcag
     9841 ttcactataa ggtaataact ctctgttcat aagtgttttt ttatctgtat caattatagg
     9901 aatatcataa tgagcatcga aagaagagtc tcctcttcca gggaaatgtt tacctgtagc
     9961 aataacattt atctccttaa atccacgaca tgcttcaatt gaatactcta caacttcatt
    10021 aactctgtct gaataagctc ttgtatatat ttcagggtta tttggctgca catttatatc
    10081 aagtaccggg gagtgaatcc aattaaaccc aactgatttg ctttgccttg caactgcttt
    10141 agctgcttca taagcaaatc tgctatcccc cgttgcccgt atacccattg acctgggaaa
    10201 gaaattaaca ccagtgaatc tagaattgac acctgcccca ccttcctgat caaaagaaaa
    10261 atgcagagga attccaaggg gtctgctcat agcaacaccc tggagttcat caagtattct
    10321 tttatactca aaaactgata catatggtgg acttattcct tgtttgtaac ctctggtgtc
    10381 ttctatacta acaatagttt tacctgttct gggatcaata tagcttccga aatgcctgga
    10441 acctggtgaa agccttaagc ctccacagtg gtacttagtg atgtaatcat atatatgcgg
    10501 ccttgcaatt gtacctgcaa agcctaacgt aagaagcgca ccaattttct gatctaaagt
    10561 cattttagat attagacttt ttatatattt atgttcgctt ttcatttaga aaacctccat
    10621 tttaagtgtt ctatgcagtt accaattctt tgaaatctta aatttggtta acctttataa
    10681 actgactcca tgcttttttg ttattttcat caatgcactc aactctaact acagtatctg
    10741 tttctttaat tctatataca cccttactta ttaatcctgt ttcactcgca gtaattctgt
    10801 cacctgatga aaatgtatca ttaaggaata caatcttttt tacaggtgag cattctgcaa
    10861 tgattttatc tttgtctaca gttatttgtc ttatttcagg accttgtgaa gagtaaaaat
    10921 ttccagcctt aatgctttca aagatgctct ttttatccag cctctcacta ttgaccataa
    10981 tataaccacc aaatgcatca tttgtatatc gatgactgtc atcagtagca aatagatatt
    11041 ttacaacacc tttcgatgca acaacatcca cataaatgga ggcgtctcct cttaagcttt
    11101 taacatcatt agcgtgattc catatctcta taccaaaata atcatgtaaa tctattaaat
    11161 catcatgagt aatcaacgac catgcaggat gagcaattat tgctattcca ttctttttat
    11221 ttatttcatc aataattgtc tgaagtttcg tattatcatc tgtatatatt tcttcctcaa
    11281 tacctatac
//
LOCUS       DAOSDD010000141        11212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2222_length_11212_cov_5.459622, whole genome shotgun sequence.
ACCESSION   DAOSDD010000141 DAOSDD010000000
VERSION     DAOSDD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2222_length_11212_cov_5.459622"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..495
                     /locus_tag="PK733_12365"
     CDS             118..495
                     /locus_tag="PK733_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOJ11371.1"
                     /translation="MPRPMKWRKVCCLPESNKFGPLDLPADAKNYVNMTVDEYETIRL
                     IDLEGFTQEECANQMNVARTTVQGIYIEARKKLAESLVNGKVLFIEGGEYRLCDGLGN
                     GCGRGCHRHGRGRHFTDNEDRGD"
     gene            559..1313
                     /locus_tag="PK733_12370"
                     /pseudo
     CDS             559..1313
                     /locus_tag="PK733_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051398.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            1447..1806
                     /locus_tag="PK733_12375"
     CDS             1447..1806
                     /locus_tag="PK733_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ11372.1"
                     /translation="MIKIAVASENEMVTEHFGHCINFNIFETENNQIVQSESIPNPGH
                     KPGFLPNYLNDMGVNVIISGGMGSGAIDIFNEKGIEVIVGASGNAKAAAEAYLQGSLK
                     STGSVCHEHQHHDECGE"
     gene            complement(2305..2724)
                     /locus_tag="PK733_12380"
                     /pseudo
     CDS             complement(2305..2724)
                     /locus_tag="PK733_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
     gene            complement(2729..3076)
                     /locus_tag="PK733_12385"
     CDS             complement(2729..3076)
                     /locus_tag="PK733_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ11373.1"
                     /translation="MSLFGRKKEEKTSCCCGGNCDAESMEKAESAKSEGASVKVLGSG
                     CAKCNQLEAATKAALQQLGMDTAIDHVTNFSQIAAYGVMTTPALVVDGKVVSYGKVLK
                     TDEVVKILQKIRG"
     gene            complement(3197..3415)
                     /locus_tag="PK733_12390"
     CDS             complement(3197..3415)
                     /locus_tag="PK733_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ11374.1"
                     /translation="MAKTWYPVIEYLTCVECGICVANCPHGVYDSAKAPSPVVTNPEA
                     ALAIATDAGIVARLEQLPMLAMIPAGHR"
     gene            complement(3431..4444)
                     /locus_tag="PK733_12395"
     CDS             complement(3431..4444)
                     /locus_tag="PK733_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ11375.1"
                     /translation="MQVLKAIWDFFQNQILGMKWLNALIGNMLSALGLNTSNRWIGSI
                     QFFIYDVIKIVVLLCFLIFFISYIQSYFPPERSKKILGQFHGIGANSVAALLGTVTPF
                     CSCSSIPLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGTKVAILYVIFGLA
                     IAIIGGTLIEKSHMEKHVESFILTAGSVDIESPDLAKKDRLIYAKEQTLSTFKKVFPY
                     ILIGVGIGAIIHNWIPEAWVVSLLGSNNPFGVILATLIGVPMYADIFGTIPIAEALLF
                     KGAQLGTVLSFMMGVTTLSLPSMIMLRKAVKPELLALFIAFCTIGIIFVGYFFNVIQG
                     FII"
     gene            complement(4535..4846)
                     /locus_tag="PK733_12400"
     CDS             complement(4535..4846)
                     /locus_tag="PK733_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ11376.1"
                     /translation="MEIAYKEHAKVFKAFCDEKRLRILDLLCGGEKCACVLLEQLDLG
                     QSGLSYHMKILVESGVVESRQKGKWTHYKISEKGSAYAITLLEELTTPNTVIEENIYG
                     I"
     gene            5170..5533
                     /locus_tag="PK733_12405"
                     /pseudo
     CDS             5170..5533
                     /locus_tag="PK733_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
     gene            5812..6096
                     /locus_tag="PK733_12410"
     CDS             5812..6096
                     /locus_tag="PK733_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOJ11377.1"
                     /translation="METRVAVIGIVVENVESVEKLNSILHEYGEYIIGRMGIPYRTKK
                     INIISIAIDAPQDIISALSGKIGRLPGISAKTAYSNISAKSNINKKIEEK"
     gene            6096..7136
                     /gene="hydE"
                     /locus_tag="PK733_12415"
     CDS             6096..7136
                     /gene="hydE"
                     /locus_tag="PK733_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOJ11378.1"
                     /translation="MKQLVDKLKQSQSLSFDEFKLLINNRTADIAEYLFENARRVRIE
                     NYGRDVYMRGLIEFTNYCKNDCYYCGIRKSNCNAERYRLTKEQILDCCAQGYELGFRT
                     FVLQGGEDGFYTDKKIVDIVSSIKGKYSDCAVTLSIGEKSFESYKAYFDAGTDRYLLR
                     HETANAEHYGKLHPNSLSLHNRKQCLFNLKEIGYQVGCGFMVGSPFQTTECIVEDLLF
                     IKELSPHMIGIGPFIAHKDTPFAVEPSGTLDLTLFLLGIIRLMLPKVLLPATTALGTI
                     DPCGREKGILAGANVVMPNLSPTNVRKKYMLYDNKICTGDEAAECLMCMQQRIESIGY
                     KLVVSRGDYKFV"
     gene            7337..8755
                     /gene="hydG"
                     /locus_tag="PK733_12420"
     CDS             7337..8755
                     /gene="hydG"
                     /locus_tag="PK733_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOJ11379.1"
                     /translation="MYNPKSLEAEEFICHEEVLETLEYAAKNKNNVELIDSIIEKAKL
                     RKGLSHKEAAVLLDCEIEEKVKKIYDLAEQIKKDIYGNRIVMFAPLYLSNYCVNGCVY
                     CPYHLKNKHIVRKKLSQEDIVREVTALQDMGHKRLAIESGEDPVMNPIEYILECINTI
                     YSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKESYEKLHPTGPKH
                     NYAYHTEAMDRAMQGGIDDVGLGVLFGLELYRYEFAALLMHAEHLEAVHGVGPHTISV
                     PRIKRADDIDPDVFDNGISDDVFAKICACIRIAVPYTGMIISTRESKTVREKVIRLGV
                     SQISGASRTSVGGYCEPEPEDEKSEQFDVSDTRTLDEIVRWLMESKYIPSFCTACYRE
                     GRTGDRFMSLAKNGQIQNCCHPNALMTLKEYLEDYASEETRAIGEALIKAEINNIPSN
                     KVKDIVRANLKNIENGQRDFRF"
     gene            9162..>11212
                     /locus_tag="PK733_12425"
     CDS             9162..>11212
                     /locus_tag="PK733_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ11380.1"
                     /translation="MWRLAKKCLTFVLCICVIATNVLIPGPIVNAENGAISVNNSEKA
                     DENYNSEEAGESFSITGEEPVEKVAAPAASPASGNATAGEVELSCSTEGAVIWYTLDR
                     SEPNPGDNATIQYSDTSKPSINGCTVIKAVAVKEGMQSSDTVTFVYNAIPVKVLEPDG
                     ATPVQNARVELYDANMWCQISEISDANGFAYIYIDAMYIIDGEYTIKACPIDISKYAI
                     STAQSITIENGTYKGGTIESRLNESILTGTVKIPGEENVLVTCGWIDIEKYYDQGGWQ
                     YLFGFPLQPDGTYNLPLLEDGRYRIRANPEQNTTDYDDYLSSNWQDITILEGVVTEPL
                     SAPVNLALNTVQVKGTVTYPDGTGVPAFCYDIRIFDTNSGVEYFEYIGHITNNTYRIS
                     GLPHGNYSVKAIARNTPYTDSVKENFEINAENSFHTIGLQLTNPQLIITIKEPDGNNI
                     VSGGNVEVLDTTPEHKWICSVPVAQDGTVKIGGLNTGTYYIKANPPHNSGYSPSALTG
                     IEISSTSPGAPEEITIKLTGKQLKLKWISSYEWDGKELRLRLDNISLLNGTNGNPDYS
                     NVSVDITSEGNTLYTLFNPYQGGRWDEEADLVFDAQSIAKKLPCGRYTIIAKNNNEPI
                     AYENNKLDVFQRIIAMPFVTTLDEYKANGITLTIDTETVDNSKQPPAWGTDQVKVKVT
                     A"
BASE COUNT         3670 a         2070 c         2315 g         3157 t
ORIGIN      
        1 atatattaca aaaccgcctc gaagttattg ataagcaatt agagaaccta taatgggcat
       61 cagaggtaac tggaggacgc tctagttatt tctgcttaga attgaggtga taagattatg
      121 ccaagaccaa tgaaatggag aaaagtatgc tgcttacctg aaagcaataa atttgggcct
      181 cttgatttac ctgcggatgc aaaaaattat gtaaatatga cagttgacga atatgaaacg
      241 ataagactta tcgatctaga ggggtttaca caagaagagt gtgccaacca gatgaatgtc
      301 gctcgaacta ccgtccaagg tatttatatc gaagcgagaa agaagctggc tgaatcacta
      361 gtgaacggta aggtgctttt tattgaaggc ggcgaatacc ggctttgtga tggattagga
      421 aatggatgtg gccgaggctg tcataggcat gggcgtggta gacactttac agataatgag
      481 gatcgaggtg actaattata tattctgaaa aagtaattga acattttatg tgtcctcaaa
      541 atacctatag tgcgccagaa cctgcacctt ataacaacac aaaacgctga accgaagaaa
      601 acagattatt atggtttgcc aaaagcggag ataaatccag aactgtgtat tgaatgcaat
      661 cagtgcagac aaaactgcag atttgatgcg atttcagcag ataggaaata caaagtggaa
      721 ccttatgcct gtgaaggctg tggggtttgc gaggctgttt gtccggcaga agctatagtt
      781 ttaaaatctg ttgtatctgg tgagttgatg ttgtatgcaa atgaggaaga agttttttct
      841 acagcacagc tgagaatggg tagtggcaca tctggaatgc tggtcacaga ggtgaaaaaa
      901 caaataaagt cggcagcagc ctctaccaaa ctagcaatta ttgacggttc tcccggcata
      961 ggttgccctg ttatagcatc acttagcggt gtagatatgg ttttgatagt ggccgaacct
     1021 tccatgtcag gtattaatga tatggagagg attataaaaa ctgcagcaaa gtttgggact
     1081 aaaacagcaa tctgtataaa caaatttaac actaacattg aaacacagaa aaaatagaag
     1141 aattttgtaa aaggcaagga ctttccttta ttggaagaat accatttgat tctgacgcag
     1201 taagggctat caataacggg aaaactatcg tagatataaa ttgtgcatcg agcatagcag
     1261 taaatgggat ctatagcaag gttatgaatc tgctgtttgg cggagaagca taacttaaaa
     1321 gttcttgcaa agtttccgat tgaccccaaa atatcagcag cttgtgatag aggtatgata
     1381 gagctttttg atggcaattg gcttgagcca gttgctaata tattagtaaa actggaggag
     1441 aaaaaaatga ttaaaattgc agtagcaagt gaaaatgaaa tggtgacgga gcattttgga
     1501 cactgtataa acttcaatat ttttgaaact gaaaacaacc agattgttca gagtgagtct
     1561 ataccaaacc ctgggcataa acctggcttc cttcctaact atctaaatga tatgggagtt
     1621 aatgtgataa tctcaggtgg tatgggtagt ggtgctattg atatctttaa tgaaaaaggt
     1681 atagaagtta ttgttggggc aagtggtaat gctaaagcag cagcagaagc gtacctacaa
     1741 ggttccttaa aatctaccgg ttccgtttgc catgaacatc agcatcatga tgaatgcggt
     1801 gaataactaa agttttgcat tataagggga aggcacacta tgtccttccc ttttgcttgg
     1861 atatgttcct gtctaccgat agcgtcaaaa atgccgtgga tatgtttccg agaaccgaca
     1921 ggtcgaaaga tattcattct gtaaaggcgg atttgatagt taagtggtcg ggttagatgt
     1981 catagcctgg tgacagagca ggttagatat ggagtactcg tgatagaaca gatttgaatt
     2041 tgataagcct tataaagccg cgagttccac tttcaaaaca tgaatttttg attcaatttt
     2101 ctttatcacg gcaatgaatt catcgtcggg catccctgtc ggatcattta agccccaacc
     2161 ctcacgacgc ttacatagca gataagggca ttctacatta cagcccatcg ttacggccac
     2221 atctataaca ggaagttcct cgagtaactt tggccgctgg gttgcttcca tgccctggaa
     2281 tgaaaatata atgaccaatg tcaaaacaaa caccatagct gtacacggag ctgccccaag
     2341 tagaattgta ccggccaaat aatctttaga gagttctccc gggataaacg ttttgaaaat
     2401 cacaaagaaa aacagccaag cgataccaaa catagtaaac ggctttatca gccagttggt
     2461 cacccatgtg acaaataatc cttttggatt ttttccaaca tcctttatgc tcttgaaatc
     2521 aaccttcagc atcatgggat agatcatgag ccaaataagt atggcaatgg gtatggatac
     2581 atttgcatac tcgaagcgcc caaggaaagc cggaatccaa ggtagaaatt tacctatcaa
     2641 cactccaatg accatacaca tagctaccca taaggtaaga tacttttcaa aaaaactaat
     2701 tcccttattt ttactgtttt ccatatgttt atcctctgat cttctgcagt atctttacga
     2761 cctcatcagt cttaagaacc ttgccgtaag aaactacttt accatctacg actagagcag
     2821 gggttgtcat gacgccgtag gccgcgatct gagagaaatt ggttacatgg tcaatggcag
     2881 tatccatgcc gagctgctga agagctgctt ttgtagctgc ttcaagctga ttgcattttg
     2941 cacatccgct tccaagcact ttcacactcg caccctcgct tttagcactc tctgcctttt
     3001 ccatgctttc cgcatcgcag tttcctccac agcagcagga ggttttttct tcctttttcc
     3061 ttccaaatag tgacatattc atactctcct ttataatttg attttattgt ttagcaacag
     3121 ctgttcccgc aggagcatcc agctttgctt ttctttcctt aggaactgca gctttccgca
     3181 gatacctgca tgccgttcag cggtgtccag ccggtatcat cgccaacata ggtaattgct
     3241 ccaaccgggc aacgattccc gcatccgtgg caatggccaa tgcagcttcc ggatttgtta
     3301 caacgggaga cggtgccttt gcggaatcat agacaccgtg aggacaattt gcaacgcaga
     3361 tgccgcactc cacgcaggtc aggtattcaa taactggata ccaagtcttt gccataatac
     3421 gttcctccta ctatataatg aatccctgaa tgacgttaaa aaagtagcct acaaaaatga
     3481 tgccaatggt gcagaacgcg atgaacagcg ccagcagctc cggcttgaca gctttgcgga
     3541 gcataatcat agatggcagg gacagggtgg tgacacccat cataaaagac agcaccgtac
     3601 ccagctgagc acctttgaag agcagcgctt cagcaatcgg aattgtgcca aagatgtcgg
     3661 catacatcgg cacgcctatt agtgtcgcta aaattacacc gaagggatta ttacttccaa
     3721 gcagggatac cacccatgcc tcgggtatcc agttatgaat aatcgcacct attccaacac
     3781 cgatcaggat gtatggaaaa acttttttaa aagtagatag cgtttgctcc tttgcataaa
     3841 tcaaccgatc tttttttgca agatccggtg actcaatgtc aacgcttccg gctgtcaaga
     3901 taaagctctc aacatgtttt tccatgtgcg atttctcgat tagagtgcca ccaattatag
     3961 cgattgccaa accaaatatt acatataaaa tggcgacttt tgtaccaaat atactcatta
     4021 gcagaacaag agagcccagg tccaccattg gcgaggaaat caagaatgaa aaagtgactc
     4081 cgagcggtaa acctgctgaa gtaaagccaa taaataatgg aatggacgag caggagcaaa
     4141 agggtgttac agttccaagc agggcggcaa cggaattcgc cccgatcccg tgaaactgtc
     4201 ctaatatttt cttgcttcgt tctgggggga agtagctctg gatatagctg ataaaaaaaa
     4261 tcaggaagca tagcagtact acaattttta taacatcata aatgaaaaat tgaatgctcc
     4321 cgatccagcg attggaggtg ttcagcccta aagcagataa catgtttcca attaatgcat
     4381 tcaaccattt catgccgaga atttggttct ggaaaaaatc ccagatcgct tttaacactt
     4441 gcataaaaca acccttcttt ctaaaaatgg acaatcatat caaaaacttt tgatgtattc
     4501 tacctgttaa aagctatcga tcagatagcc tgtatcatat gccgtaaata ttttcttcta
     4561 taaccgtgtt cggcgttgtc agttcttcta ggagagttat agcataagcg ctaccttttt
     4621 cgctaatttt gtaatgtgtc catttgcctt tctggcggct ctccacaaca cccgactcaa
     4681 ctaaaatctt catatggtat gaaagccctg actgccccaa gtctaactgt tccaacaaaa
     4741 cacaagcgca tttttcaccg ccgcacaata gatcaagtat ccgtagccgt ttttcatcac
     4801 aaaaggcctt gaatactttt gcgtgttcct tataagctat ttccaagtta tcacctcata
     4861 tcaaatatat ttgatatgtt attagtatat gcatcaaatc aaattttgtc aatgtgtttt
     4921 tgaacattat gtaaatcggt ctgcgataac aatagccttt tgaatatctg gcgaatttat
     4981 tgaaaacact tcctataaat aaaaattggc tgctatttga ctactactga ttaaaaacaa
     5041 ataattgaaa tacgaggcat tatggaaatt tactgacatt aagtattaag aattccacat
     5101 aataaataga ggatatacct tgacatcggg ttatcgtctt taatattagc ttctttgaaa
     5161 ataaaattct cctctgcaac tgcagaagat ttaccgacgg cctcctttgc aggccagcag
     5221 gatacatatt tgaacattat aggaaaggaa gcaatcctaa agcatattag caagtgctgg
     5281 gcatcgctgt ttactgagag ggcagtaaca taccgtcttc aaaatggctt cgaccaccgt
     5341 aaagtccatc tgtctgtggt tgttcagaaa atgatcttcc ctcaagcggc agggattttg
     5401 tttactgcca atcactactt tataccccat ccctgaagcg aatgaccatg aatatcacgt
     5461 ctatatatct gtgggtcatc aacaaatgat gactgacccc atgaaaccat tgggattgtc
     5521 tttatggcaa ttaaagacat tgtaaaatgc gcccttaaaa ccgaataaat gttgaataaa
     5581 taaaacatct atgttaaact aaattagcta agtgacaagt acttcttata acaaagcaaa
     5641 taaaaataat tgcttctgac actgaaatat tttgaagtga aaggcattgc aattttatag
     5701 ctagatagtt agaatgatta caatcataat aattactgta taaaattact gcgcctttca
     5761 atgggcgcat ttttaatttt gagtattttt aaggagtggt aagtgataga tatggaaaca
     5821 agagttgcag taatcggaat agttgttgaa aatgttgaat ctgtagaaaa attaaatagt
     5881 atattgcacg aatatggtga atacattatt ggcagaatgg ggattccata ccgcaccaag
     5941 aaaattaata tcataagtat agccattgat gcaccgcagg atatcatcag tgctttgtca
     6001 ggtaaaatcg gcagattacc aggtatcagt gcaaaaacag cctattccaa tataagcgca
     6061 aaatcaaata ttaacaaaaa gattgaggaa aaataatgaa acaattagtt gataaactta
     6121 aacaaagtca aagtctgtca tttgatgagt tcaaactttt aattaataac cgaacggcag
     6181 atattgcaga ataccttttt gaaaatgcaa gacgagttcg gattgaaaat tatggtagag
     6241 atgtatatat gcgaggtctc attgagttta cgaactactg caaaaatgac tgttattatt
     6301 gtggaattag aaaaagcaac tgtaatgcag aaagatatcg tttgacaaaa gaacagatac
     6361 ttgattgctg cgcacaaggc tatgagcttg gatttcgtac ttttgttcta cagggcggtg
     6421 aagacggatt ttatactgac aaaaaaattg ttgatattgt cagttccatt aagggaaaat
     6481 attctgattg tgcagtaacc ctatccatag gtgaaaagag ttttgaaagc tataaagcat
     6541 attttgatgc aggcactgac cgatatctgc ttcgtcacga aactgcaaat gcggagcatt
     6601 acgggaaact acatcctaat tccctatcac tccataaccg taagcagtgt ttgtttaatt
     6661 taaaggaaat aggttatcag gttggatgcg gatttatggt gggctcacct tttcagacaa
     6721 cagaatgcat tgtagaagat ttgcttttta ttaaagagct aagcccccac atgataggaa
     6781 ttggaccgtt tattgctcac aaagatacgc catttgcagt tgaaccatcc ggaacattgg
     6841 atctgacctt gtttttgctt ggaatcattc gtttaatgct gccaaaggtg ctgctgcctg
     6901 caacaacagc acttggaaca atagatccat gtggccggga aaaaggtatt cttgcgggag
     6961 caaatgttgt tatgcccaat ctgtcgccaa ccaatgtcag aaagaaatat atgttatatg
     7021 ataataaaat ctgtacaggg gatgaagcgg ctgaatgcct tatgtgtatg cagcagcgca
     7081 ttgaaagcat cggatataaa ttagttgttt caagaggtga ctacaaattt gtgtaggcat
     7141 atacaagtac tgtattaaat tacattattt atgttgattt gtattgactt acattaactt
     7201 atattaactt atattaacta acaataactt atgttaacat atggtaacca atgtcagcca
     7261 acattagtta tcattagata aaataaaatt aactaatgag gaagatttat ctgactcaaa
     7321 agaaaggaaa attactatgt ataacccaaa atctttagag gcagaagagt ttatctgcca
     7381 cgaggaagtg ctggaaactc tggagtatgc agcaaaaaat aaaaacaatg ttgaattgat
     7441 tgacagtatt attgaaaaag cgaagctccg caagggactt tcccacaaag aggcagcagt
     7501 gctgcttgac tgcgaaattg aagaaaaggt caagaaaatt tatgatcttg cagaacaaat
     7561 taaaaaggat atttacggta accgcattgt tatgtttgcg cccctttatt tatccaatta
     7621 ctgtgtaaac ggctgtgtat actgccccta tcatttgaaa aacaaacata ttgtgagaaa
     7681 gaagctttca caggaggaca ttgtccgaga ggttaccgca ttgcaggata tgggtcataa
     7741 gcgtcttgca attgaatccg gtgaagatcc tgtaatgaat cctattgagt atattcttga
     7801 atgtatcaat accatttaca gtataaaaca taaaaatggt gctattcgtc gtgttaatgt
     7861 gaacattgca gctacgacag ttgaaaatta cagaaagctc aaggaagcag gaatcggcac
     7921 atatatttta tttcaggaaa catatcataa ggaaagctat gaaaaactac atccaacagg
     7981 accgaaacat aactatgcat accatactga agcaatggac agagccatgc aaggtggaat
     8041 agatgatgta ggtctgggcg tgttattcgg actagagctt taccgttatg aatttgccgc
     8101 tctcctaatg cacgcagaac atctggaagc agtacatgga gtaggacctc atacaataag
     8161 cgttcccaga attaaacggg ctgatgatat tgacccggat gtttttgata atggtataag
     8221 tgatgatgtt tttgccaaaa tttgcgcttg tattcgcata gcagtgccat atacaggtat
     8281 gattatttct acacgagaaa gcaaaaccgt aagagagaag gttatccgtc tcggcgtttc
     8341 acagataagc ggtgcatcca gaaccagcgt tggcggatat tgtgaaccgg aacctgagga
     8401 tgaaaaatcc gagcaatttg atgttagtga tactagaacc cttgatgaga ttgtaagatg
     8461 gctgatggaa agcaaatata ttcccagctt ttgtaccgca tgctaccgtg aaggcagaac
     8521 aggtgacaga ttcatgagcc ttgccaaaaa cggacagatt cagaattgct gccatcccaa
     8581 tgcacttatg actttgaagg agtatctgga agattatgct tctgaagaaa ctagagctat
     8641 tggtgaagct ttgattaaag ctgaaataaa caatattcca agtaataaag tcaaggatat
     8701 tgttcgtgcc aatctaaaaa atatagaaaa cggtcagcgg gatttcaggt tttaggaaac
     8761 atagctggaa gcttctctat atttaatgaa gcagatgtat gaaacgtggg gacagttatt
     8821 ctgttttgtc ttttaaatga aacagaagga ctgtccccac gtttatattg taaaaaaatg
     8881 cttgcattaa taaatatagt attatatagt attatcaaat ggaatttctt tacgtaaaat
     8941 gttggtaatt gtataaccat ggtttttgtg aaaatttggt gaatattttt acaaaaagtt
     9001 tattattagg gtgggaggag ggtcgaatga gccggaatgc atctaatcac taaaattatt
     9061 tattaagcct tatgactaat caagtgttgt attattggac tacataatct atcttttcca
     9121 cttttaaaaa acttaataac gaaaggggag atcaagtcct tatgtggaga cttgccaaga
     9181 aatgtttgac ttttgttctt tgtatctgcg ttatagcaac caatgtatta attcccggac
     9241 ctattgttaa tgcagaaaat ggagcgatat ccgtaaacaa ttcagaaaaa gctgatgaaa
     9301 actacaattc tgaagaagct ggtgaaagct ttagtataac aggtgaagaa cccgttgaaa
     9361 aggtagcagc gccagccgcc tcgccggcat caggcaacgc tactgcaggt gaggttgaat
     9421 tatcttgctc aactgaaggt gcagtgattt ggtatacatt ggacagaagt gagccgaatc
     9481 ccggcgacaa tgcaacaatt cagtatagcg atacaagcaa accttcaatt aatggttgca
     9541 ccgtaataaa agcagttgct gtcaaagaag gaatgcaaag cagtgatacg gttacttttg
     9601 tgtacaatgc aatacctgta aaagtattgg aacctgacgg tgctactccg gtacagaatg
     9661 ccagagtgga attatacgac gcaaacatgt ggtgtcaaat cagtgagata tcagacgcca
     9721 acggatttgc gtatatttat attgatgcaa tgtacataat tgatggtgaa tatacaataa
     9781 aagcatgtcc gattgatatc tcaaaatatg ctataagtac tgctcaaagt attaccatcg
     9841 aaaacggcac ctataaaggc ggtactattg aatcaagatt aaatgaatca atacttaccg
     9901 gcactgttaa aatacctggt gaggaaaatg tgttggtaac ttgcggatgg atagatatcg
     9961 aaaaatacta tgatcaaggt ggctggcaat atctatttgg atttccgctc caaccggatg
    10021 gtacatataa tcttccactg cttgaggacg gcagatatag aatcagagca aatcctgagc
    10081 agaatactac agattatgat gactacttaa gttcaaattg gcaggatata accatattgg
    10141 aaggagtggt aaccgaacct ttgtcagcac ctgttaatct tgcgttgaat actgtgcagg
    10201 ttaagggtac tgttacatac cccgacggaa caggagtacc ggcattttgt tatgatattc
    10261 gtatctttga tacgaattcc ggagttgaat attttgaata cataggtcat attacaaata
    10321 atacatacag gatatcagga ttgccgcatg gtaattattc agtaaaagca atagcccgca
    10381 atacgccata tacagattcc gttaaggaaa attttgaaat aaatgctgaa aattcttttc
    10441 atactatagg cttacaactg acaaatcctc aattaattat cactattaaa gagccggacg
    10501 gtaataacat tgtttctggt ggaaatgttg aagtacttga tacaacaccg gagcacaaat
    10561 ggatttgcag tgttcctgta gctcaagatg gaaccgttaa gatcggcgga cttaataccg
    10621 gaacatatta tataaaagca aacccgccgc acaattcagg atatagtcct tcagctctca
    10681 ccggaattga aatatcgtct acttctccag gtgctccgga ggaaattacc attaaattaa
    10741 cagggaagca gctaaaactt aagtggatct catcttatga atgggatggc aaagagctta
    10801 gattaagact tgataacatc agtcttctga atggcaccaa cggcaatcct gactatagca
    10861 acgtctctgt tgacataact tcagaaggca atactttata tactttattc aacccatacc
    10921 agggtggcag atgggatgaa gaagcagatc ttgttttcga tgcacagtct attgcaaaaa
    10981 agcttccctg tggacgatac acaattattg caaaaaacaa taatgagccg atagcttatg
    11041 aaaataataa gcttgatgta tttcaaagaa ttattgcaat gccttttgtt acaacccttg
    11101 atgaatataa agcaaatggg ataacactaa caatcgacac cgaaacagtt gataacagca
    11161 aacagcctcc tgcatggggt actgatcaag taaaggtgaa agttacagca ga
//
LOCUS       DAOSDD010000142        11167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2233_length_11167_cov_5.381479, whole genome shotgun sequence.
ACCESSION   DAOSDD010000142 DAOSDD010000000
VERSION     DAOSDD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_11167_cov_5.381479"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /gene="fliD"
                     /locus_tag="PK733_12430"
     CDS             <1..390
                     /gene="fliD"
                     /locus_tag="PK733_12430"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ11381.1"
                     /translation="AIMDLFSRKSDKYPSYSRTLNSEQKTQRYGESGLAHRLFDIIED
                     NISTMRNADGKKGYLLEKAGMEGDASEFRNTIYDEMIDYDKRIDSLYEKLIKKENSYF
                     MKFAQMEKIIGQMNAQSNWLMMQLGKN"
     gene            427..810
                     /gene="fliS"
                     /locus_tag="PK733_12435"
     CDS             427..810
                     /gene="fliS"
                     /locus_tag="PK733_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOJ11382.1"
                     /translation="MALNKAYNQYMENSIFTSTPEELTLMLYNGLVKFIMQAQVSIEE
                     KQIEKANNSILRAQSIITELQSTLDMKYEVSKNMMLLYDYIYNRLIDANIKKDKEILE
                     EVMGFAKELRDTWTQAMKIAKKEKL"
     gene            904..1446
                     /gene="flgN"
                     /locus_tag="PK733_12440"
     CDS             904..1446
                     /gene="flgN"
                     /locus_tag="PK733_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOJ11383.1"
                     /translation="MTIEEYINQLDDLLEEKYESLIEILSLTRLQSDALEKEDVAALE
                     KLVEEKQVKINKINLLDEKFSRIFEQVKRQFAIDRIDELDESKELNITGAKNLNITGV
                     KSLNITGVKSLKGKTSKILDIINNEISVLEKKNNESILELMNRYKMEFKNIVSGKKAL
                     SAYNNNFSTHTPSFYIDKKK"
     gene            1621..3474
                     /locus_tag="PK733_12445"
     CDS             1621..3474
                     /locus_tag="PK733_12445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ11384.1"
                     /translation="MESNKNIEGNTKQNSFNELLPGERYKLVKILGSGGMSTVYLAEN
                     INLGTYWAIKKISKNSNTSVDILVEPNILKKLNHPALPRIIDIMEDNEYLYIVQDFIE
                     GNPLDKELRKNGKFPLRNVVEWALQICDVLIYLHSFEPNPIIYRDMKPANIILTPWGE
                     IKLVDFGIAREYKKGRSNDTVNIGTIGYAAPEQFGGGQSNITTDIYSFGVTLFQLITG
                     KELQEFSLGIKLPGHYADGINLQMEQIICKCTRFNPEERYQSVEQLKKDIESVRNSFL
                     KHKAEIADSQTESINTSKIMNIMNTMNTLNTANTTNTLNTANVAGETGNEAIDADTAP
                     EIENMRSKAFWKRKKQSRIMHFKRMIITVWDNTEFACEMAYIAAKATDFEILLVDLDL
                     LSPKADLFLNIKKYPEIILREGILNESGLNIVLDSAERGYLSRENIIGACIKRKDLKN
                     LYILTGNYKLDNYEYYSNDSLTQFIDKACQIFDIIVLAVNKSIYDLFTTISLIKSDIN
                     IIPIHAVVDKFREFNNYIVFLGDKQQIPLEKNKFIAYEYDSRVHLPVSVIEEATQYNY
                     LGSVKYCPRREKYRNLQIAYARKMDKKIEYDYISILEKLKVLPELKVLHEL"
     gene            3522..4814
                     /locus_tag="PK733_12450"
     CDS             3522..4814
                     /locus_tag="PK733_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ11385.1"
                     /translation="MPIISTLHKNLLDSGEKFIFEKDKSDLSTIISNITFNILRDSPG
                     LVADVANGQVDGHVLETAIIKDIDKNKYHYGVFRDELIKKVLDFMFGYGELQQYIEDE
                     DVTDIDGTRYNEFVIKRNGIREKVFVNFGNEKIFDTYCKLIAIRNGGILNENDSHCRV
                     TDEKRHLRINVSVKPRNISGPAISIRKHRLKSLDLNDLIKLGMINEEIKELILKLAMS
                     DATVIFCGKGAAGKTTLMRAFVNTLPEMERILVVEADAEVYPDKPYCIEQRVKKENEG
                     GRPVTLRDLVKDGLTMSLDSYCIGEIVGDEAWEFIKASFTGHRGMATIHSESAEDTFS
                     RLLILSKGANIQESEKTIKEMMAKSIDVIFYLKSFKVIDALEVVDYDHIEDKFKYNRL
                     LEYRIKYEDYNGNLEGQFIKCSNVGKRLSNIFARRGLA"
     gene            4811..5782
                     /locus_tag="PK733_12455"
     CDS             4811..5782
                     /locus_tag="PK733_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ11386.1"
                     /translation="MSFGIKVLFVINSMSLAFLTGFLSLQIAGNIKFKKAIKENINKL
                     NKNYNEKRLLRELRKYTGLSVARVSVVEKIELYLIDKSNIKNYIPFVNFYILLLCCTL
                     IFIISFRSVYKITLFIPSTVILCCLASLTPVFILDIMGRYNSEKIRKKLAGFISVLNR
                     WCAVKEDIFHAFEKSIESGVSEPLKTYIRDMIVQVNRGVEPMEALSILQMKVDNPQFR
                     DFIINIKQNVKHRGDIRNLLENMEEQFYKIEEEFNRRKISTYKDRIVIYCIMVLVLII
                     GYAFLKLNPEVEQFYLATYQGKTLLTVFCMLYALGFYLTLGITKFDV"
     gene            5799..6722
                     /locus_tag="PK733_12460"
     CDS             5799..6722
                     /locus_tag="PK733_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ11387.1"
                     /translation="MDVMTMIKEIIIFSEKHKLFTILMLIVNSVLGILITWKIALITN
                     KSIYEAMGRRMIDKSLYKKCAYIITRYVEDYERRSEKSGLFIKAKNRAKKLGFKGEHS
                     TSKYLFVRYGISIIIFIVSFIINFPSAKESIKASIIVFITSELIIRRKRGELNNSLQK
                     YVYKIYKYLHNQIASGIKVTDAIKNLYEVIDDKDLKEVLIRMAARYELTTDIDAALNE
                     FISVYDSQEAESFCIAIKQGILTGDNSELLARQEDIMFKKYFNYIQSETDSCRTRSFI
                     AAIVFTAIIVVMILVPIFFEASGALTKIFIS"
     gene            6828..7067
                     /locus_tag="PK733_12465"
     CDS             6828..7067
                     /locus_tag="PK733_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11388.1"
                     /translation="MKKYARIINSVIFRCKNINQKLLNNRKGFGMNEILGIAAAIVIA
                     ALIVLPGLKTFTGDIMEKLTTWWDGVQSVFFSTTP"
     gene            7097..7474
                     /locus_tag="PK733_12470"
     CDS             7097..7474
                     /locus_tag="PK733_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11389.1"
                     /translation="MPSKALIYGITLVMLAAVLVFMVEIFLPLSAKNEFDGICRGTIM
                     QMEVRGGLTNTTRDNLLNALRDKGFSNLKVQGTDNARYGEEIFLKVEGDYIYSRLIGL
                     FTRRYIVKHIVYDRVTIARKVVN"
     gene            7474..7986
                     /locus_tag="PK733_12475"
     CDS             7474..7986
                     /locus_tag="PK733_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11390.1"
                     /translation="MEKGSSISDVLIGAAIIIFIILPVFSAVVERYILFAKVQIIKDA
                     VDITNISAYNALKTDSLGKTTVDFHNDKISGIYKKILAGNMKLDLDLNPTESSVADDT
                     VIIKSIIIYTENIPNICPYGTSILRPAVHSCIVVPVKPTIYSRIIRKITGKNTFKLEV
                     HVDSDIPINN"
     gene            8073..8747
                     /locus_tag="PK733_12480"
     CDS             8073..8747
                     /locus_tag="PK733_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOJ11391.1"
                     /translation="MKRVNILILTIILVVILLIIEVIIVRSAAEYEPKVNAVFAAVDI
                     LAGEIIKDEMLEKREISISLVHQKSFKTKDEIIGKKAKNDMEKGEMILAGRIYTDGEI
                     DEIQVLDSKNRLFTVEFKGDQVNGWWLKEGQYVDIIFIPSERIQKESVHGIENNNIQR
                     VINVRVAAIIDDKGKLVKGMENASTPKYISFEVDDRLDEFLAYAKGNGRIEVSVIPDM
                     EGLQNK"
     gene            8761..8997
                     /locus_tag="PK733_12485"
     CDS             8761..8997
                     /locus_tag="PK733_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HOJ11392.1"
                     /translation="MAEIKYDIIKNLGTIGYGSKGWKKEVSIVSWNERKPKLDIRDWD
                     ENHEKMGKGITLSREEALELKEILNNMDFDEIND"
     gene            9116..10006
                     /locus_tag="PK733_12490"
     CDS             9116..10006
                     /locus_tag="PK733_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11393.1"
                     /translation="MDDRALPKAWESLMNSADFVPIIVRTVERFHDTSWVMEPNRHEF
                     FEMVYIKKGNVIFEISGESAEIGPNEMIIIKPKQDHKFIVKSHSGCEFVVCNFKFENR
                     VRNDISEVSLEDFLNFVRDRETGAFITLKVSAKNDIIMLLDRILKEKQSDEPGTEFLN
                     YLLVLELFVLISRALKMEWENSIKGKSTKLKEVIQAAVSFINTNYERDISLRDISKYV
                     FLSPSYFAKAFKEETGKSPISYLIQVRLNRAKELLAATEQKVGEIALAVGFSNQQRFN
                     EIFKKYTGMTPLQYRKTWHG"
     gene            10288..>11167
                     /locus_tag="PK733_12495"
     CDS             10288..>11167
                     /locus_tag="PK733_12495"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOJ11394.1"
                     /translation="MGMTMTQKILADHAGLDNVVPGQLIKAKLDMILGNDITTPVAVT
                     EFRKIGVDRVFDVNKIAIVPDHFTPNKDIKSAEQVKFIREFSKEMGIVNFFEVGQMGV
                     EHALLPEKGLVVSGDVVIGADSHTCTYGALGAFSTGVGSTDMAAGMATGEAWFKVPEA
                     IKFVLEGKPGKWVSGKDIILHIIGMIGVDGALYKSMEFIGDGIQHLTMDDRFTIANMA
                     IEAGAKNGILIVDEKTLEYVREHSTKPYKVYSADDDAEYSMSYIIDLASIKPTIAFPH
                     LPENTRTIDQVGNIRID"
BASE COUNT         4175 a         1453 c         2305 g         3234 t
ORIGIN      
        1 gctattatgg atcttttcag caggaaatct gataaatatc cttcatacag cagaacattg
       61 aactcagaac aaaaaactca gcgatatgga gaatcaggcc ttgcccacag gctttttgat
      121 ataattgaag ataatatatc aacaatgaga aatgccgatg gcaagaaagg ctatctcctt
      181 gaaaaggcag gaatggaggg agatgcatca gaattcagga acactattta tgatgagatg
      241 atagattatg ataaaagaat cgattctttg tatgaaaaac ttataaaaaa agaaaacagt
      301 tattttatga agtttgcaca aatggaaaaa atcataggac agatgaatgc acaaagtaac
      361 tggctgatga tgcaacttgg taaaaattaa tgatcacaaa tttgatttat ttttggaggg
      421 cgaaaaatgg cattaaacaa agcatataat cagtatatgg agaattcaat atttacttca
      481 actccagagg agcttactct tatgctctac aacggcctgg taaaatttat tatgcaggca
      541 caagtatcaa tagaggagaa acagattgaa aaggcaaata acagtatatt acgggcacaa
      601 tctataatta cggaacttca atctacactg gacatgaaat atgaagtatc aaaaaatatg
      661 atgcttcttt atgattacat atacaacagg cttatagatg ctaatataaa gaaggataag
      721 gaaatacttg aagaggttat ggggtttgca aaggaactga gggatacctg gactcaagct
      781 atgaaaattg ctaagaaaga gaaattatag aagattggat ttagggaaca gaatgtatgg
      841 aagctagagg ctaataggca cttaaaattc tgaaaaaatg cattgctgaa ggagcgcaca
      901 ggtatgacca ttgaagaata tattaaccag cttgatgatt tactggaaga aaagtacgaa
      961 tctttgatag aaatattgag tttaaccagg ttgcagtcag atgctttaga aaaggaagat
     1021 gtggctgcac tggaaaaact tgtagaggaa aaacaggtta aaattaataa aataaacctg
     1081 ctggatgaaa aattcagcag gatttttgaa caggttaaaa gacaatttgc tattgataga
     1141 attgatgaat tggatgaatc aaaggaactt aatataactg gagcaaaaaa ccttaatata
     1201 actggagtaa agagccttaa tataactgga gtaaagagcc ttaaggggaa aacttcgaaa
     1261 attcttgata taattaacaa tgaaataagt gtattggaaa agaagaacaa tgaatcgata
     1321 ttagagctta tgaatagata caaaatggaa tttaaaaata tagtatccgg taaaaaggca
     1381 ctctcggcct ataataacaa cttttctacc cacacacctt ctttttatat agataaaaaa
     1441 aagtaatgga agtatatcta aaattatttt attctttata tgaaaattta aacaagcacg
     1501 aattggaaaa ataatatata atatgtaaaa taatagttga catgtaatat attattatgt
     1561 ataattgtaa ataagataca aaatatacat attaaagtat aaaatttgca gggtggggtt
     1621 atggagagta acaaaaatat tgaaggaaat actaagcaaa attcttttaa cgaattgctt
     1681 ccgggagaaa ggtacaagct tgtaaaaatt ttaggaagtg gtgggatgag tacggtgtat
     1741 ctcgctgaaa atataaacct gggtacttac tgggcaatta aaaagatatc taaaaactct
     1801 aatacaagtg tagacatatt agttgagcct aatattttaa aaaagctcaa tcatcctgct
     1861 ttgccaagga ttattgatat tatggaagat aatgaatatt tatatattgt tcaggatttt
     1921 attgaaggca atcctcttga caaagagttg cggaaaaacg ggaaatttcc gctgagaaac
     1981 gttgttgaat gggctttaca gatttgtgac gtacttatat atcttcattc atttgaacca
     2041 aaccctataa tttacagaga tatgaaacct gcaaatatta ttttaacacc ctggggtgaa
     2101 ataaaactgg tggattttgg catcgccagg gaatataaaa aaggacgaag caatgatacg
     2161 gtcaatattg gaacaatagg ctatgcagcc cctgagcaat ttgggggtgg tcaatcaaat
     2221 ataacaacag acatatacag ttttggtgta actttattcc agttgataac agggaaggag
     2281 ctacaggaat tttcccttgg tataaaactt ccaggacatt atgcagatgg tattaatcta
     2341 caaatggagc aaataatctg taaatgcaca agatttaatc ctgaagaaag atatcaatcg
     2401 gtggagcagt taaaaaaaga catagaatct gtaagaaatt catttttaaa acataaagca
     2461 gaaattgctg atagtcagac agagtcaatt aatacaagta aaataatgaa tataatgaat
     2521 acaatgaata cactgaatac agcaaataca acaaacacac tgaacacagc gaatgttgca
     2581 ggtgaaacag gtaacgaagc gatagatgca gatacagctc cagagataga aaatatgaga
     2641 agtaaagctt tttggaagag aaaaaaacaa agcagaatca tgcattttaa aaggatgata
     2701 ataacagtgt gggataatac agaatttgca tgtgaaatgg cgtatattgc agctaaagca
     2761 acagactttg aaatactgct tgttgacctt gatttgcttt cccccaaagc tgacctgttt
     2821 ttaaatataa aaaagtaccc tgaaataatt ttgagagaag gcatactaaa tgaatcaggg
     2881 cttaatattg ttctggattc tgcagaaagg ggctacctct cacgggagaa tataataggg
     2941 gcttgtatta aacgtaagga tttgaagaat ttatatattt taaccggtaa ttacaagctt
     3001 gataattatg aatactacag taatgatagc ctcacccaat tcattgataa ggcatgccag
     3061 atttttgaca ttattgtcct tgctgtaaac aaatcaattt atgatttatt tacaaccata
     3121 tctcttataa aatctgacat aaacattata cctatacatg cggtcgttga taaattccgg
     3181 gagtttaaca attatattgt gtttttgggg gataaacaac agatacctct tgaaaaaaac
     3241 aagtttatag cttatgagta tgattctaga gttcatctgc ctgttagtgt aattgaggaa
     3301 gcaacacaat ataattatct tggaagcgta aaatattgtc ccagaagaga aaagtatagg
     3361 aatttacaga tagcatatgc caggaaaatg gataagaaga tagagtatga ctatatatcc
     3421 atacttgaaa aattaaaagt actaccagaa ctaaaggtac tgcatgaatt ataggctctg
     3481 ctataaatta aaatcaatgc catgaaagga aggtgccatt tatgcctatt ataagtaccc
     3541 ttcacaaaaa tctgcttgat agtggagaga aatttatttt tgaaaaagat aaaagtgatt
     3601 taagtacaat aatatcaaat attactttca atatattgcg cgattctccc ggactggtgg
     3661 ctgatgtagc caatggccag gtagatggac atgtgttgga aacagctata attaaagata
     3721 ttgataaaaa caaatatcat tatggggtct tcagagacga attgataaaa aaagtacttg
     3781 attttatgtt tggctatggc gaattacagc aatatataga agatgaagat gtaacagata
     3841 tagatggtac aagatataat gaatttgtaa ttaagcgaaa tggaataaga gagaaggtct
     3901 ttgtgaattt tggtaatgag aaaatattcg atacttattg taaactaatt gcaataagaa
     3961 atggtggaat acttaatgaa aatgacagcc actgcagagt aaccgatgaa aaaaggcacc
     4021 ttcgcataaa tgtttcagta aaaccaagga atatttccgg gcctgccata agtatacgca
     4081 agcacaggct taaaagcctg gacttaaatg acctcataaa acttggcatg ataaatgaag
     4141 aaatcaaaga gttaatcctg aaattagcca tgtcggatgc tacagttatt ttctgcggga
     4201 aaggtgctgc aggaaaaacg acccttatga gagcgtttgt aaatactctt ccggaaatgg
     4261 agaggatttt ggtagtagaa gctgatgctg aggtgtaccc tgataaacct tattgtattg
     4321 agcagagggt gaagaaagaa aatgagggcg ggaggccggt tacactccgg gatcttgtaa
     4381 aggatggact taccatgtct ctggattctt attgtattgg ggaaatagta ggcgatgaag
     4441 cttgggagtt tattaaggct tcatttacag gtcatagggg tatggctaca attcactccg
     4501 aaagtgctga agacacattc tccaggctgc tgattctaag taagggagct aatatacagg
     4561 aaagtgaaaa gactataaaa gaaatgatgg cgaaaagtat tgatgttata ttttatctta
     4621 aatctttcaa agttatagat gccttggagg tagtagatta tgaccatata gaagacaaat
     4681 ttaaatacaa caggttgttg gaatacagga ttaaatatga ggattataat ggaaatttgg
     4741 agggacaatt tatcaagtgt tccaatgtag gtaaaaggtt aagcaatatt ttcgccagga
     4801 ggggactggc ttgagctttg gtataaaagt gttatttgta ataaattcta tgtccctggc
     4861 ttttttaacc ggatttctct ctttacaaat tgccggaaat atcaaattta aaaaagccat
     4921 aaaagaaaat ataaataagt tgaataagaa ttataatgaa aaaagattgc tgagagaatt
     4981 aagaaaatat accggcttga gtgttgcaag agttagtgtg gtagaaaaaa ttgagttata
     5041 tcttatagat aaatcaaata taaaaaatta tattccattt gttaactttt atatattact
     5101 gctatgttgt actttgattt tcattataag ttttaggtct gtgtataaaa taacactatt
     5161 cattccatca acagtaatac tttgctgtct ggcttctctt actcccgtat tcatacttga
     5221 tataatggga agatacaatt ctgaaaaaat acgtaagaag ctagctggat ttatttctgt
     5281 gttaaatagg tggtgtgctg taaaggaaga tatattccat gcatttgaaa agtccataga
     5341 atcaggggtt agtgaacctt taaagactta tataagagat atgattgttc aggtaaacag
     5401 aggggtagag cctatggaag cattgtctat attgcagatg aaggtagata atcctcaatt
     5461 tagagatttt atcattaata taaagcagaa tgttaaacac agaggcgata tcaggaattt
     5521 attagaaaat atggaagagc aattttataa aattgaggag gaatttaaca gaagaaaaat
     5581 atcaacttat aaagaccgta tagtaattta ttgcataatg gttctggttt taataattgg
     5641 atatgcattt ttaaaactta atccggaggt tgagcagttt tatcttgcaa catatcaggg
     5701 gaaaactctt ttaacagtgt tctgtatgct ttatgctttg ggtttttatt taactttggg
     5761 gataactaaa tttgatgtat agcataggag taatatcaat ggatgtaatg acaatgataa
     5821 aagaaataat catattttca gaaaaacata aattatttac tattctaatg ctcattgtca
     5881 actctgtttt aggtatctta attacatgga aaatagcttt aataactaat aaaagtatat
     5941 atgaagctat gggaaggagg atgatagaca agtcactata taagaagtgt gcatacataa
     6001 taacaagata tgttgaagac tacgaaagga ggagtgaaaa atcaggttta tttataaaag
     6061 caaaaaacag ggctaagaag ttaggattca agggtgaaca ttcaacatcc aaatatctgt
     6121 tcgtgcggta tggaatttca ataataatat ttatagtttc atttattatt aatttccctt
     6181 ctgcaaagga atctattaaa gcttccatta ttgtatttat tacaagtgaa ttaattatta
     6241 gaaggaaaag aggagaactt aacaattcac ttcaaaaata tgtttataaa atctataaat
     6301 acttacataa tcagattgct tcaggtatca aggttaccga tgcaataaaa aacctgtatg
     6361 aagttataga tgataaagat ttgaaggaag tattaattcg aatggcagca agatatgaac
     6421 tcactactga tattgatgcg gctttaaatg aattcatatc tgtatatgac tcccaggagg
     6481 cggaaagttt ctgtatagca attaagcagg gaatattgac cggggataat agtgaacttc
     6541 tggcaaggca ggaggatata atgtttaaaa aatacttcaa ctatatacaa tctgaaacag
     6601 atagctgtag aacaaggagc tttattgccg caattgtctt cactgcaatt attgtagtta
     6661 tgatacttgt gccaatattt ttcgaagctt ccggagcatt gacaaagata tttataagct
     6721 gatatgaaag tagtattaac tcaatattgg cgaaagaata tgacttttta cttgccatac
     6781 ataaaacatt aactatgatt tagaaaagaa atgggggaat cattactatg aaaaaatatg
     6841 caaggataat caatagtgta atttttagat gtaaaaatat aaaccaaaaa ctattgaata
     6901 atagaaaagg ctttggtatg aatgaaatat tgggtatagc ggcagctatt gttattgctg
     6961 cactaatagt gctaccgggc ttgaaaacat ttacaggcga tattatggaa aaactaacta
     7021 catggtggga tggcgtacag agtgtattct tttccacgac tccgtagaaa atgagaagca
     7081 gcaatgggag gtagttatgc catctaaggc cttgatttat ggaattactc tggtaatgct
     7141 tgcggctgta cttgtattta tggttgaaat atttctgcca ctatctgcaa aaaatgaatt
     7201 tgatggaata tgcagaggaa caattatgca aatggaagta aggggtgggc tgaccaacac
     7261 cacgcgggat aaccttttaa acgcattgag ggataaaggg tttagcaatt tgaaggtaca
     7321 gggaactgat aatgccagat atggagaaga aatatttctt aaggtggaag gggattacat
     7381 atatagcagg ttaataggtt tattcaccag aagatatata gtaaaacata tagtatatga
     7441 tagggtgacc atagcaagaa aggttgtaaa ttaatggaga agggtagcag tatatcagat
     7501 gtattgatag gagcggcaat aattattttt attatacttc ccgtattttc agcagttgtt
     7561 gaaaggtata ttctttttgc caaagtacaa attataaaag atgctgtaga tataacaaat
     7621 atatcggctt ataatgcttt aaaaacggat agcttgggga aaaccaccgt agacttccat
     7681 aacgacaaga tttcaggcat atataaaaaa atacttgcag gtaatatgaa actggattta
     7741 gaccttaacc caaccgaaag ctctgttgca gatgatactg taataataaa atcaattata
     7801 atttatactg aaaatattcc aaatatctgt ccatatggga caagcatttt aagaccggca
     7861 gtacatagct gtatagtagt accggtcaag ccaaccattt attcacgaat tatcaggaaa
     7921 attacaggga aaaatacttt caaactggag gttcatgttg attctgacat tccaataaac
     7981 aattagtttt atgggggaat agagtctagt ttgaaaggaa tagagtctag tttgaaagga
     8041 atggagccta gtttgaaagg aatggagctt aaatgaaaag ggtaaacatc ttaatattga
     8101 caattatact tgttgtgata ttactgataa tagaggtaat tattgtaaga agtgcagcag
     8161 aatatgaacc aaaggtaaat gcagtattcg cagctgtgga tatattggca ggcgaaataa
     8221 taaaagatga aatgcttgaa aaaagggaga taagtataag tctcgttcac caaaagtcgt
     8281 ttaaaactaa agatgaaata ataggtaaaa aagcaaagaa tgatatggaa aagggtgaaa
     8341 tgatactggc aggcaggatc tatactgatg gtgaaattga cgaaattcag gtccttgaca
     8401 gtaaaaacag attatttaca gttgaattta aaggagatca ggttaatggt tggtggttga
     8461 aagaaggtca atatgttgat attatattta taccctctga aagaattcaa aaggaaagtg
     8521 ttcatggtat agaaaataat aatattcaaa gggtaataaa tgtaagggta gctgcaataa
     8581 tagatgataa aggcaagttg gtaaaaggta tggaaaatgc atctaccccc aagtatatat
     8641 cttttgaggt tgatgacagg ctggatgaat ttcttgcata tgcaaaggga aatggaagaa
     8701 ttgaggtttc cgtaatacct gatatggaag gacttcaaaa taaataaagg aggtagcagt
     8761 atggcagaaa taaaatatga tattattaaa aacctgggga ctataggtta tggttccaaa
     8821 gggtggaaaa aggaagttag catcgttagc tggaatgaaa gaaagcccaa gctggacata
     8881 agagattggg atgaaaacca tgaaaaaatg ggaaaaggta taacccttag cagagaagag
     8941 gctcttgagt taaaagaaat attgaacaac atggattttg acgaaattaa tgattaggga
     9001 aaaagccttc accataatag gtgagggcat ttccatcttt caatgtgttg acgctgaatg
     9061 tgtatttatg tcataattat agtaaataaa ctaccgagaa agaatgtggt tttctatgga
     9121 tgacagggcc cttcccaaag catgggaaag cctgatgaat tcagctgatt ttgttcctat
     9181 aatcgtaaga acggttgaac gcttccatga tacaagttgg gtcatggagc ctaacaggca
     9241 tgagtttttt gaaatggtgt atataaaaaa aggcaatgtt atttttgaga tttcaggtga
     9301 atcagcagaa attggcccca acgaaatgat aataataaag ccgaaacagg accataaatt
     9361 cattgtaaaa tcccactcag gatgtgagtt tgtcgtatgt aattttaaat ttgaaaacag
     9421 ggtaagaaat gatatttccg aggtatcttt ggaggacttt ttgaactttg ttagagacag
     9481 ggaaacaggt gcatttataa cattaaaagt aagtgcaaag aatgatatta ttatgctttt
     9541 agataggatt ttaaaagaga aacagagcga tgaaccaggg acggaatttc taaactacct
     9601 gcttgttctg gaactttttg tgcttatttc gagagctctt aaaatggaat gggaaaacag
     9661 cataaaaggt aaaagtacta agcttaaaga agttattcag gctgctgtca gttttataaa
     9721 cacaaattat gaaagagaca tttccctaag ggatatatca aagtatgtat ttttgagtcc
     9781 aagctatttt gcaaaggctt ttaaggaaga aacagggaaa agccccataa gttatttgat
     9841 acaggtaagg cttaacagag caaaggagct gcttgcagcc actgagcaaa aagttggtga
     9901 aattgccttg gccgtaggct tctcaaatca acagaggttt aatgaaatat ttaaaaagta
     9961 tactggcatg accccattgc aatacagaaa aacctggcac ggatgatttt atttcatagg
    10021 catgaatcca cataattcca gtaaaagtct tgtgctgtca ggtatacaaa aagaaacgtt
    10081 aataatttct taaaataaaa aataaataac ggaaggattt ccttaattaa taatttataa
    10141 ttgatattga atttatgatg aaataataac tataactata aattagcata aattagtata
    10201 aattaatata attttgtata gctataaatc agagtagttg gggaataact gcctgattca
    10261 tgagattaaa aaggaggctg atgccgaatg ggaatgacaa tgacacagaa aatcctggct
    10321 gatcatgcag gtcttgataa tgtagtaccg gggcagctta tcaaggccaa gcttgatatg
    10381 atactgggaa atgatattac aacacctgta gcagttactg aattcaggaa aataggagtt
    10441 gatagggttt ttgatgtaaa caaaattgca atagttcctg accattttac acccaataaa
    10501 gatattaagt ctgcagagca agtaaaattt ataagagaat tttcaaaaga gatgggaatt
    10561 gttaatttct ttgaagtggg acaaatgggt gttgagcatg ccctgcttcc tgaaaaagga
    10621 cttgtggtgt ccggggatgt tgtcattggg gcagattccc atacatgcac atatggtgct
    10681 ctgggcgctt tctcaacagg ggttggaagc actgatatgg cagcaggcat ggctacagga
    10741 gaagcctggt tcaaagtacc tgaagcaatt aaatttgtgc tggaagggaa acccggtaaa
    10801 tgggtaagtg gtaaggatat tatcctgcat ataataggta tgataggcgt tgatggagct
    10861 ttgtacaaat caatggagtt tattggagac ggaattcagc accttacaat ggatgaccgc
    10921 tttacaattg ccaatatggc tattgaggca ggagcaaaaa atggtatatt aattgtagat
    10981 gaaaagactc ttgaatatgt aagagaacat tctaccaagc cttacaaggt ttattccgct
    11041 gatgatgatg ccgaatattc catgtcatac atcattgacc ttgcatcgat aaagccaaca
    11101 atagcatttc cgcatcttcc ggaaaataca agaacaatag atcaagtggg taatataaga
    11161 atagatc
//
LOCUS       DAOSDD010000143        11155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2240_length_11155_cov_5.651261, whole genome shotgun sequence.
ACCESSION   DAOSDD010000143 DAOSDD010000000
VERSION     DAOSDD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2240_length_11155_cov_5.651261"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..339
                     /gene="rpmH"
                     /locus_tag="PK733_12500"
     CDS             205..339
                     /gene="rpmH"
                     /locus_tag="PK733_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOJ11395.1"
                     /translation="MLRTYQPNNRKRKKIHGFRQRMKTKQGRDVLKRRRLKGRKVLSA
                     "
     gene            458..805
                     /gene="rnpA"
                     /locus_tag="PK733_12505"
     CDS             458..805
                     /gene="rnpA"
                     /locus_tag="PK733_12505"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOJ11396.1"
                     /translation="MKKTVSIKSNRDFVRVYKKGRFVAGRYIVLYLLNNVLDINRLGI
                     TASKKTGKSIKRNRIKRLVRENYRLGEDMVKSGYDLVFVIRSVNEMPDFYSIKREMNY
                     LMRRLGIIVEENK"
     gene            837..1046
                     /gene="yidD"
                     /locus_tag="PK733_12510"
     CDS             837..1046
                     /gene="yidD"
                     /locus_tag="PK733_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOJ11397.1"
                     /translation="MKNVLIGAIRLYQKYISPLKPPSCRFYPSCSQYALDAIKKYGSI
                     KGTFMSVRRILKCHPFNPGGYDPVK"
     gene            1088..1975
                     /locus_tag="PK733_12515"
     CDS             1088..1975
                     /locus_tag="PK733_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOJ11398.1"
                     /translation="MGFLDFIAKPLGQFLYIIYNSVAFKNYGLAIIIFTVVIKLILLP
                     LTIKQYRSTAKMQEIQPIIQDIQRRYKNDKEKMNQELMKVYQEHKYNPAGGCLPLLIQ
                     MPILLSLYWVINQPLKYMLNKTPEQITSLVNYVTEHVGKVAMSYSKEIGVFNYFNAHI
                     SELSNVGDLLKPEELINLNFLGMNLGIIPSIDTKLLFGPESAVYLPLLLIPILSVLTT
                     WFSTKLMTPKAEEGSKSDAASSTQGTMALIGPMMTLLFSFQLPAGVGLYWIASNLFQI
                     FQQLYLNKYVLKKKEGAVK"
     gene            1976..2596
                     /gene="jag"
                     /locus_tag="PK733_12520"
     CDS             1976..2596
                     /gene="jag"
                     /locus_tag="PK733_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOJ11399.1"
                     /translation="MDYVVEKTGKTVDEAVSLALEDLGVDDKNVEVEVLEEGNKGIFG
                     IIGARMAKVRVTRKESSAEKVSKYLQEIFDKMGVNVNMTVEDSDNSIFVKIEGEDSGI
                     IIGRRGETLDALQYLAGLAANNGKEDYKRVVIDIENYRRKREETLVKLSNKLAERVIR
                     SRKSITLEPMSPYERRIIHSALQNNKYIRTFSVGDEPNRKVVITLK"
     gene            2661..4031
                     /gene="mnmE"
                     /locus_tag="PK733_12525"
     CDS             2661..4031
                     /gene="mnmE"
                     /locus_tag="PK733_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOJ11400.1"
                     /translation="MYDNDTIAAISTPYGTGGIGIIRVSGDKAFEIVGKIFKGKKDVG
                     QFKSHTINYGKIKDPLDDSIIDEVLLTKMDKPITFTRENVVEINCHSGITILKKILEL
                     LIREGARPAEPGEFTKRAFLNGRIDLAQAEAVIDIINARTLKSSKVALQQLEGKLSEK
                     IKNTRNNLVELIAKIEVTLDYPEEDVDKITEQEVYNSLIEIRRKLMDLLSSFKKGRII
                     RDGISAVIVGRPNVGKSSLLNELTGQNRAIVTEVPGTTRDIIEEYINISGIPVKLLDT
                     AGIRETGDIVEKIGVDLTKKAIEAADLVIMMIDAAEGFTEEDEKILKILQGKKIIILT
                     NKIDLKNDVSHIPETDGISIIKTSMTKGIGIDQLENEITRLFLSGNIFIENEGLVTNL
                     RHANLIEKAIKSVDNGISAYNDGMPLDCMTVDIRNAVHELGEITGESINEDIINEIFS
                     RFCIGK"
     gene            4070..5986
                     /gene="mnmG"
                     /locus_tag="PK733_12530"
     CDS             4070..5986
                     /gene="mnmG"
                     /locus_tag="PK733_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOJ11401.1"
                     /translation="MEYLAGEYDVAVVGAGHAGCEAALAAARLGCKTVIFATNLDSIA
                     NMPCNPNIGGTGKGHLVREIDALGGEMAKAADKALIQLKILNSAKGPAVYSLRAQIDR
                     RKYQIIMKDTLEMQENLDIKQAEIVDIIVDNVDNEEGKKKVTAVKTHTGAKYLCKTLI
                     LATGTYLKGKIIIGDVSFNSGPDGLFPANKLSDSLRENGVELFRFKTGTPARINRRSI
                     DFSKMTEQPGDDRIIPFSFETESIERQQVSCWLTYTNSETKKIIKDNLHRSPLYSGVI
                     EGIGTRYCPSLEDKIVRFAEKEAHQVFVEPMGLETQEMYLQGMSSSLPEDVQIEMIKT
                     VPGLENVSIMRIGYAIEYDCVNPAQLDLSLELKDVGGLFCAGQINGSSGYEEAAAQGI
                     IAGINAAQKAKGKAPLILDRSEAYIGVLIDDLVTKGTREPYRIMTSRAEYRLLLRQDN
                     ADLRLTSKGYEIGLISKERYDKFLRKKEQIEEEISRLKNTTVPPDEKVNSFLTDRDST
                     LLKSGAKLSDLLKRPEITYEDLAEIDPDRPELNYAVREEVAISVKYEGYIKRQLQQVE
                     QFKKLENRKIPEDIDYEKIKNLRIEAKQKLGKIRPLSIGQASRIAGVSPSDISMLLIY
                     LEQIKRGGGSPSNQ"
     gene            6011..6790
                     /gene="rsmG"
                     /locus_tag="PK733_12535"
     CDS             6011..6790
                     /gene="rsmG"
                     /locus_tag="PK733_12535"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOJ11402.1"
                     /translation="MTNSGYSEELRGLLTDGAGEFGVELNSAQIDKFFKYMELLKTWN
                     GKINLTSIEDDRDIILKHFIDSISIVPTTNKSININMRSMTNTSEGYYPQVIDIGTGA
                     GFPGIPLKIISQEINITLLDSIDKKVKFLEECIAYLDLSKIRAFHGRAEDYAHKAGHR
                     EFYDIAVARAVANLPVLLEYCLPFVKEGGVFIAMKGRNIDEISDSNKALDILGGKIEE
                     IREINLPFTDNMRNIIIIRKFRQTPTKYPRKAGKPSKIPLI"
     gene            6935..7771
                     /gene="noc"
                     /locus_tag="PK733_12540"
     CDS             6935..7771
                     /gene="noc"
                     /locus_tag="PK733_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HOJ11403.1"
                     /translation="MLENRIQTLDPAGGNTIENIIYININNIRPNPYQPRKHFNKSSL
                     EELCESIKQYGVLQPINVRKIGTSHYELVAGERRLRASAMAGLDKIPAIVVNVDDNDS
                     AILALIENLQREDLNYLEEAEGYNNLIVEHNLTQEELALRIGKSQSTIANKIRLLKLS
                     PLIKKILIDNNLTERHARALLKLHDEQLQLKVLRYVCEKGLNVKRTEELVEKVIERYS
                     SRITTKNSGKRTNKAIKDIRIFVNTIKHAVDIMKKSGLNAKAAQFDRGEYFEFVVRVP
                     KN"
     gene            8226..10790
                     /gene="gyrA"
                     /locus_tag="PK733_12545"
     CDS             8226..10790
                     /gene="gyrA"
                     /locus_tag="PK733_12545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOJ11404.1"
                     /translation="MTNGEERIEQNIIPVDIESEMKKCFIDYAMSVIADRALPDVRDG
                     LKPVHRRILYSMYLLGFTPDKPHRKCASTVGEVLGKFHPHGDAAVYDSMVRMAQDFSL
                     RYTLVDGHGNFGSRDGDAPAAMRYTEARLSKIAMEMLRDINKDTVDFKPNFDEHEMEP
                     VVLPSKFPNLLVNGSSGIAVGMATNIPPHNLGEVIDGLIQVIDNPDITIDELIKTIKG
                     PDFPTAGIIIGRQGIRDAYRTGKGRIVVRAATNIEQMSGSKQRIIVSELPYQVNKARL
                     IEKIAELVKDKKIEGISDLRDESDRNDPVRIVIELKREANANVVLNQLYKNTQMQDTF
                     SANMLALVQTPEGKYEPKVLNLKETLTYYIAHQKDVIRRRTAFDLEKAEAAAHILEGL
                     RIAIDNLDEVIHIIRTSKNEASAKVRLMEAFNLSDRQAQAIVDMRLGRLTALEREKLE
                     AQYNELLEKIKYYRDILANESMVFSIIKEELTEVKTKYADERRTSINIDEGEIDEEDL
                     IDQEEVAITLTHFGYIKRLPADTYRSQKRGGRGVHGITTREEDFVEDFFITSTHDYIL
                     FFTNMGKVYRMKAYQIPEAGRQARGMAIVNLLEISQDEKITAVIPVSEYREGLYLTMA
                     TKQGLVKKTNLMEYDNIRRGGLAAVSLREQDELIEVKLTDGQCDIILVTYKGISIRFN
                     ESNVRPTGRVSQGVKGIALDEDDYVIGMGVSMEDAYMLIVTENGYGKRTLLDEYRAQI
                     RGGKGVITYKITEKTGNIAGMRIVNEDDDIMLINKDGTIIRINVSEISVLGRSTQGVT
                     LMRIDEDSRVVGLARVEGSYEDESRNGFDDEDDDEYDNRDGDGYSNGDDDVNGEGD"
BASE COUNT         4098 a         1541 c         2405 g         3111 t
ORIGIN      
        1 atctgtgtcg tgaattacga atttttcctt gccttaaaca taacaatccg taagccttgt
       61 tcaaatttca tactcggaaa atcgtatgct gccatatttt gtatcatagg aagaaaatgc
      121 gcctatttta aaaaatactt tcttgacact agtatcactt ataatatata attttgctga
      181 tgcttataaa atggaggtgc aaatatgctt agaacttatc aacccaataa tagaaaaaga
      241 aagaaaatac atggctttag gcagagaatg aaaacgaaac agggtagaga tgttttaaaa
      301 aggcgtagat taaaaggtag aaaagtactt tctgcataag accgcataaa agtggtctat
      361 tttttctatt caattaaaat gcttgtttta agggctttta gattatagta attttgatta
      421 taataatacg gtacttaata acaggcgaga ggattgtatg aaaaaaactg tttcgattaa
      481 gagcaaccgc gattttgtca gagtgtataa gaaaggaaga tttgttgctg gaagatatat
      541 tgttttatat ttgttgaata atgtacttga tattaacaga ttaggtataa ctgccagtaa
      601 aaaaaccggc aagagtataa aaaggaacag gataaagcgg cttgtaagag aaaactacag
      661 acttggcgag gatatggtga aatcaggcta tgatttagta tttgtcataa gaagtgtaaa
      721 tgaaatgccc gatttttaca gtattaagcg tgaaatgaat tacctgatga gaaggctggg
      781 tataattgtt gaggaaaata aatagctttt acaagaataa tattttaagt caaatactga
      841 agaatgtttt aattggtgca ataagactat atcaaaagta catttcacct ttgaagcctc
      901 caagttgcag gttttatcca agctgttccc aatatgcttt agatgccata aaaaaatatg
      961 gctcaataaa agggactttt atgtctgtta gaaggatttt aaaatgccac ccttttaatc
     1021 cgggaggcta tgatcctgta aaataagtga gttaccattg tgtaaattag agagggagaa
     1081 tcagaaaatg ggttttttag attttattgc taaacctctg gggcagtttt tgtatataat
     1141 ttataattca gtagccttta aaaactatgg tttagctatt attatattta ccgttgttat
     1201 taagctaatc ttattgcccc ttacaattaa gcaataccgt tcaactgcta aaatgcaaga
     1261 aatacagcct ataattcagg atattcagag acggtataaa aatgataagg aaaaaatgaa
     1321 tcaggagctt atgaaggtat atcaagaaca taagtataat ccagcaggag gatgtcttcc
     1381 ccttcttatt cagatgccta ttttgttatc tctctattgg gtaataaatc aacctctgaa
     1441 atatatgctt aataaaaccc ctgagcaaat tacaagtctc gtaaactatg taactgagca
     1501 tgttggaaag gtagctatga gttactccaa ggaaatagga gtttttaatt attttaatgc
     1561 tcatatttcg gagctttcta atgtaggcga tttattgaag cctgaagagt tgataaactt
     1621 aaactttctc ggaatgaacc ttggtattat accaagtatt gatacaaagc tcctttttgg
     1681 acctgagtca gctgtatatt tacctctttt actgattccc atattatctg tattaacaac
     1741 ctggttttca acaaaactta tgactccgaa agctgaagag ggttctaaaa gtgatgcagc
     1801 ttcaagtacc cagggaacta tggctttaat tgggcccatg atgacattat tattctcttt
     1861 tcagttacct gccggtgttg gtttatattg gatagcaagt aacttatttc aaatattcca
     1921 gcaattatat ttaaataaat atgtgctcaa aaagaaggaa ggtgcggtaa agtaaatgga
     1981 ttatgtggtt gaaaaaacag ggaagacggt agatgaggca gttagtctgg cccttgaaga
     2041 tctaggtgtg gatgataaaa atgtagaagt ggaagttttg gaagaaggca ataaaggaat
     2101 atttggtatt ataggtgcca gaatggctaa agttagagtt accaggaaag aatcatccgc
     2161 agaaaaagta agcaaatatt tacaggaaat atttgacaaa atgggtgtaa atgtaaatat
     2221 gactgtggaa gacagtgata attccatttt tgtaaaaata gaaggagagg atagtggaat
     2281 tattatcgga cgtaggggag aaacactgga tgctttgcag tatctggcag gtttggcagc
     2341 aaataatgga aaagaagatt acaaaagggt tgtaatagat atagaaaatt atagaagaaa
     2401 aagggaagaa acccttgtaa aattgtcaaa caagctggca gaaagagtta ttagaagcag
     2461 aaaaagcata acccttgaac ctatgtcacc atatgaaaga agaataatac attccgcatt
     2521 gcagaataat aagtatataa ggactttcag tgtaggtgat gaaccgaaca gaaaggtagt
     2581 tataacacta aagtaacatc gattaatagt gatttgaaat taattatact ggagcagcaa
     2641 ggagttatta taatttattg atgtatgata atgatacaat agcagcaatt tctaccccat
     2701 atggtacggg tggtatagga atcataagag tgagcggaga taaagctttt gaaatagtag
     2761 gaaaaatttt taaaggcaaa aaggatgtag gacagtttaa atcccatact attaactatg
     2821 gaaaaataaa ggaccccctg gatgattcaa taatagacga ggttctcttg actaaaatgg
     2881 ataagccaat tacatttacc agggaaaacg tagttgaaat caactgccat agtggcatta
     2941 ccatattaaa aaagatcctt gagttgttga tcagggaagg ggcaagacct gctgaacctg
     3001 gcgaattcac aaaaagagct tttttgaatg gaaggataga tcttgctcaa gcagaagctg
     3061 ttattgatat tattaacgcc agaactttga aaagttcaaa agttgcattg cagcagctgg
     3121 agggtaaact gtccgaaaaa atcaaaaaca caagaaataa tcttgttgag cttattgcaa
     3181 aaatagaagt aaccctggat taccctgagg aagatgtgga taaaatcacg gagcaggaag
     3241 tctataatag tctgattgaa attagaagga agctcatgga cctgctttcg agttttaaaa
     3301 aaggcaggat aataagggat ggaattagtg ctgtaatagt tggaaggcca aatgttggga
     3361 aatcatccct gcttaatgag ctaacagggc aaaacagagc aattgtaaca gaagtaccag
     3421 gaacaacgag agatattatt gaggagtata taaatattag cgggatacct gttaaattgc
     3481 tggataccgc aggtataaga gaaacagggg atattgtgga aaaaatagga gttgatctga
     3541 ctaaaaaggc aatagaagca gcagaccttg taattatgat gatagatgcc gctgaggggt
     3601 ttactgaaga agacgaaaaa atactaaaaa ttctacaagg taaaaagata ataatattaa
     3661 ctaataaaat agatcttaaa aatgatgtat cccatatccc ggaaacagat ggtattagta
     3721 ttattaaaac ttcaatgacg aaaggcatag gaattgatca gttagaaaat gaaataacaa
     3781 ggcttttctt aagtggcaat atttttattg aaaatgaggg tttggttaca aatctcaggc
     3841 atgcaaatct tattgaaaaa gctataaaaa gtgtagataa tggcatttca gcatataatg
     3901 atggaatgcc attagactgt atgaccgttg atataagaaa tgctgttcat gaattgggag
     3961 aaataaccgg ggaatctata aatgaagata ttataaatga aatattcagc agattttgta
     4021 tagggaagta acaggtgttt ccccttatca ctggaaagga agattatata tggagtatct
     4081 tgcaggtgaa tatgatgtag cagttgttgg agccggacat gcaggatgcg aggctgccct
     4141 ggccgctgcc aggcttggat gtaaaacagt tatatttgct acaaatcttg atagtatagc
     4201 caacatgccc tgcaacccca atataggagg tacgggaaaa ggccatctag tcagagagat
     4261 agacgctttg ggcggtgaaa tggcaaaagc tgcagataaa gcacttatac aattaaaaat
     4321 actaaatagc gcaaaaggac ctgctgttta ttctctgcgt gctcaaattg acagaagaaa
     4381 ataccagata ataatgaaag atactctgga aatgcaggaa aatcttgata ttaagcaggc
     4441 agagatagta gatattattg tggataatgt ggataacgaa gaagggaaaa agaaagtaac
     4501 tgctgtaaaa acccacacgg gtgcaaaata tttgtgtaaa accttaatac ttgcaacagg
     4561 cacatatcta aagggaaaaa taattattgg tgatgttagt ttcaacagcg gcccggatgg
     4621 acttttcccg gcgaacaagc tctctgatag tttaagagaa aatggtgtgg agctatttcg
     4681 ttttaaaaca ggtactcctg caagaataaa cagacgcagt attgattttt caaaaatgac
     4741 tgagcagcct ggggacgatc gtattatacc tttttcattt gaaacagaaa gtatagaaag
     4801 acagcaggta tcatgctggc tgacttatac aaacagtgaa acgaaaaaaa taattaagga
     4861 taacctccat agatccccat tatacagcgg agtaattgag ggaattggaa caaggtattg
     4921 tccttcattg gaggataaaa ttgtaaggtt tgcagaaaag gaagcgcatc aggtttttgt
     4981 agaaccaatg ggcctggaaa cgcaggaaat gtatcttcag ggtatgtcta gcagtttacc
     5041 ggaggatgtt cagattgaaa tgataaaaac agtaccaggc cttgaaaatg tatcaataat
     5101 gagaattgga tatgctatag agtatgactg tgtaaaccct gcacagcttg atctttcact
     5161 tgaattaaaa gatgtaggcg gtttgttttg tgcgggacag atcaatggaa gctcaggtta
     5221 tgaagaagct gcggcacagg ggattattgc cggaattaat gcagcacaaa aagctaaagg
     5281 aaaagctcca cttattcttg atagatcaga ggcctatatc ggtgttctta ttgatgatct
     5341 ggttacgaaa ggaacaaggg aaccatatag aattatgact tcacgggcgg aatacagatt
     5401 gcttttaagg caggacaatg ctgatttgag gcttacttca aaaggttatg aaataggctt
     5461 gataagtaag gaaagatacg ataaattttt aagaaaaaaa gagcaaatag aagaagaaat
     5521 ttcaaggctt aaaaatacca cagtaccacc agatgaaaaa gttaacagtt ttttgacgga
     5581 cagagacagt acactactaa aaagtggtgc aaaactttcc gatttactta aacgaccgga
     5641 aattacctat gaagatttag ctgagattga ccctgacagg ccggaattaa attatgcagt
     5701 aagggaagag gtagctatat ctgtaaagta tgaaggttat ataaaaaggc agctgcagca
     5761 ggtagagcag tttaagaaac tggaaaacag aaaaattcct gaagatattg attatgaaaa
     5821 gataaagaac cttagaattg aagcaaaaca aaaacttggg aaaatacgtc cattatcaat
     5881 aggacaagca tcaagaattg ctggtgtttc tccctctgat atctccatgc ttctaatcta
     5941 tctcgaacag attaaaaggg gcggaggcag tcctagtaac cagtagataa agaaatatgg
     6001 aggtagactt atgactaatt cagggtattc tgaagaattg cgtggattac taaccgatgg
     6061 agcaggagaa tttggggtag aattgaattc tgctcaaata gataaatttt ttaaatatat
     6121 ggaattgctg aaaacatgga atggaaaaat aaatttgact tccatagaag acgatagaga
     6181 tattatttta aagcatttta tcgattctat tagtattgtg cccactacaa ataagagcat
     6241 taatattaat atgagatcaa tgacgaatac atcagaaggg tattacccgc aagttataga
     6301 cataggtaca ggagcagggt ttccgggtat acctctgaaa ataatatccc aggagattaa
     6361 tataacttta cttgattcca ttgataaaaa agttaaattt cttgaggaat gtatagctta
     6421 tcttgattta agcaagataa gggcttttca tgggagagcg gaggattatg cccataaagc
     6481 cgggcatagg gaattttatg atatagctgt ggcaagggct gtagcaaatt tacctgtgct
     6541 tcttgaatat tgccttcctt ttgttaaaga gggcggtgtt tttatagcaa tgaaaggtag
     6601 aaatattgat gagatttctg attcaaataa ggcattagat atacttggag ggaagataga
     6661 ggaaatcagg gaaataaatt taccctttac agataacatg agaaatataa ttattataag
     6721 aaagtttcga caaactccga caaaatatcc tagaaaagca ggaaaacctt cgaagatacc
     6781 gttgatataa cctgtaaaat gcagtaatat agcatattat aaggaacgat tcttaaatta
     6841 aaaaaaaggg ataaaaaagc aatgcggcga atatgttctt gagagcttaa aattttattg
     6901 taatttaatc gaatatacaa aggatggtgc cgcaatgctt gagaatagga ttcaaacttt
     6961 agaccccgca gggggtaata ctattgaaaa tattatttat attaatataa ataatattag
     7021 accaaaccca tatcagccta gaaaacattt caacaaatca tctcttgagg agctttgtga
     7081 atccataaaa caatatggag ttcttcaacc cattaacgta agaaaaatag gcaccagcca
     7141 ctatgaactg gtagctgggg aaagaaggct gagagcatct gctatggcag ggttggataa
     7201 aatacctgct attgttgtta atgtagatga caatgactct gcaatactag cgcttattga
     7261 gaaccttcaa agggaagacc ttaattacct ggaagaagcg gagggttata ataatcttat
     7321 tgtcgagcat aatctaacac aggaggaact ggcattaaga ataggtaaaa gtcagtctac
     7381 aatagcaaat aaaataaggc tcttgaagtt atctcctctt atcaaaaaaa ttcttattga
     7441 taacaatctt acggaaagac atgcaagagc tttgctaaaa ctgcatgatg aacaactgca
     7501 gcttaaagta ttaaggtatg tatgtgaaaa aggattgaat gtaaagagaa cagaggagct
     7561 tgttgaaaag gtaattgaaa ggtattccag tagaataact acaaaaaata gcgggaagag
     7621 aacgaacaaa gcaattaaag atataagaat atttgttaat acaattaagc atgcagttga
     7681 cataatgaaa aaatccggac ttaatgcgaa ggcagcccaa tttgaccggg gagagtactt
     7741 tgaatttgtt gttagagttc caaaaaatta agcgataaat tgagtgtact ttatataatg
     7801 tttgatagca ggttatttct tgtatgaaat atttaccgga gatgacctgc tattttattt
     7861 taagcaatta taagcaatta aaagtagaca gtacataaaa actttattaa aatagatatt
     7921 ggtgtatata atgcataaaa aatgcattat ataaattttg tggtccgtaa tagattattt
     7981 ttagggttag tggaccacaa cattaaggaa gcagatttgt aacgcttgaa taagaactat
     8041 aaaaacgaga tgttacaaac tgctagtgcg cctatgttgc cttagtgaat acgcgaaaat
     8101 ccaatttgtt acttgtagaa tcatttttgc gttatatata gattggagtt aatatatata
     8161 aataaaggac aaaatatatg ttataaacac ctggtatttg attgaagaaa aggagtatat
     8221 tttaaatgac aaatggtgaa gaacgaatag aacaaaacat aatacctgtt gatattgaat
     8281 cggaaatgaa aaagtgcttt attgactatg ctatgagcgt tattgctgac agagcacttc
     8341 ccgatgttag agatggtcta aagcctgttc acagaagaat attatactct atgtatttat
     8401 taggttttac accggataag cctcatagaa aatgtgcatc cacagttgga gaagtgcttg
     8461 gaaaatttca tccacatgga gatgccgcag tttatgatag tatggtaagg atggctcagg
     8521 acttttcact caggtatacc cttgttgatg gtcatggtaa ttttggctca agggatggag
     8581 atgctcctgc agcaatgagg tatacagaag caagactttc aaaaattgcc atggagatgc
     8641 ttcgtgatat aaacaaggat actgtagact tcaagcctaa ctttgacgag catgaaatgg
     8701 agcctgttgt gctgccatct aaatttccca atttgcttgt gaatggttcc tccggtattg
     8761 cagtaggaat ggctacaaac atacctcccc ataatttagg tgaagtaata gatggactta
     8821 tacaagttat agataacccg gatattacta tagatgaact gattaaaacc ataaaaggac
     8881 ctgatttccc tacagccggt attatcattg gaaggcaggg tatcagagat gcatatagga
     8941 caggcaaggg ccgcattgtt gtccgggcgg caacaaatat tgagcagatg agtggttcaa
     9001 agcagcgcat tattgtctct gaattgccat atcaggtaaa taaggcaaga ttgattgaaa
     9061 aaattgcgga acttgtaaaa gataaaaaaa tagaaggaat atctgattta agagatgaat
     9121 cagatagaaa cgaccctgta aggattgtta ttgagcttaa aagagaagca aatgcaaatg
     9181 ttgtattgaa tcagctatat aagaatactc agatgcagga tacattcagt gctaacatgc
     9241 ttgctctggt tcaaaccccg gaagggaaat atgagcctaa agttcttaac ttaaaagaaa
     9301 ccctgactta ttatattgct catcaaaagg atgtaataag gcgcagaact gcttttgatc
     9361 ttgaaaaagc cgaagctgcc gctcatattt tagaaggatt aagaatagca attgacaatc
     9421 ttgacgaagt aatccacatt atcagaacat caaaaaatga agcatcagcc aaggtaagac
     9481 ttatggaagc atttaaccta agcgacaggc aagctcaggc aattgttgat atgaggcttg
     9541 gaaggcttac tgctcttgaa agggagaagc ttgaagcgca atataatgaa cttttggaaa
     9601 aaataaaata ctacagagat attctggcca atgagtccat ggtttttagt ataataaaag
     9661 aagagcttac cgaggttaag acaaaatacg ccgatgaaag acgtacttcc attaatatag
     9721 atgaaggaga aattgacgaa gaagacctga tagaccagga agaagtggct attactctta
     9781 ctcactttgg atatattaaa agactgcctg cagatactta cagaagtcaa aagcggggag
     9841 gcaggggtgt acatggaata accacaagag aagaggattt tgttgaggac tttttcatta
     9901 cgtccaccca tgattatatt ttatttttta caaacatggg taaagtatac agaatgaaag
     9961 catatcagat acctgaagcc ggacgtcagg ctagaggaat ggctatagta aaccttctgg
    10021 aaataagcca ggacgaaaaa attactgcag taataccggt atcagaatat cgggaggggc
    10081 tataccttac tatggctaca aaacaaggat tggtaaagaa aacaaatctg atggaatatg
    10141 acaatataag gagaggcggc cttgcagctg taagtttgag agaacaggat gagcttatag
    10201 aagttaagtt aactgatggt cagtgtgata ttattctggt tacttataaa ggtatatcca
    10261 taagatttaa tgaatcgaat gtgcgtccca ccggaagagt ttctcaaggt gttaaaggta
    10321 ttgctcttga tgaagatgat tatgttattg gtatgggagt atcaatggaa gatgcatata
    10381 tgctcattgt tacagaaaat ggatatggca aaagaacgtt gctggatgag tatagagctc
    10441 agataagagg cggtaaagga gttataacct acaaaattac cgagaaaacc ggcaacattg
    10501 caggtatgcg tattgttaat gaagatgatg atattatgct tattaataaa gatggtacta
    10561 ttatacgtat aaatgtaagt gaaattagtg ttttgggaag atctactcaa ggagttactc
    10621 taatgagaat tgatgaagat agtagagtag taggcctagc gagggttgaa gggagttatg
    10681 aagacgagag caggaatgga tttgatgatg aagatgatga tgaatatgat aatagagatg
    10741 gtgatggata cagcaatgga gacgatgatg taaatggaga aggagattaa ttgattataa
    10801 aattgattta gcaagaatat aatagtattt atgaggtaaa ttaaatatat attaaaaaaa
    10861 catgttatcc tcaggttgat aatgcacctg tcaaggtgct atattataaa agctgcttgc
    10921 acacgcagct tttgattttc aacctgattg ctggttaatg ccggttggtg gttgaagcac
    10981 aaaaatagtt tgaaaaaact aaaaaaaaag tgttgacaaa aaatattcta catgataata
    11041 tgtataagct gctcgtgagg acaacataaa aagaaacaat ataatagagc agataaacat
    11101 tatggacatt gaaaagtgaa cagtgtaaaa acaaagaact cgtcaataaa cacct
//
LOCUS       DAOSDD010000144        11097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2256_length_11097_cov_4.841152, whole genome shotgun sequence.
ACCESSION   DAOSDD010000144 DAOSDD010000000
VERSION     DAOSDD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2256_length_11097_cov_4.841152"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK733_12550"
     CDS             complement(<1..380)
                     /locus_tag="PK733_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11405.1"
                     /translation="MVKTGLVSVTFRKLSPGDIIKLVSEAALDGIEWGGDIHVPHGDT
                     KKAEEVAQMTQDAGLSVAAYGSYYYLGCEEEKKLPFERVLETAIELKAPVIRVWAGNR
                     ASSNADQAYLDMAFAEASRIADMAL"
     gene            complement(415..1278)
                     /locus_tag="PK733_12555"
     CDS             complement(415..1278)
                     /locus_tag="PK733_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ11406.1"
                     /translation="MSWDIIMHVNYCEQGQTVEEICQKAVAWGFDGVEFRRKRSGVNE
                     SVEEYLDSIEKGVRKTGLKAVIFGGPGANLSDPDSNKREVEIEEMTNFFKLASRRFNL
                     SVCNTMAGGLFNSDKSIPYSEYDKHGSFAAAEEQWKWAAEGFKVLGKLAEELGFRFAF
                     EAHMNYVHDLPAAARKLVDMIGSPAVGVNLDYGNTVYFKNIPSLKEAINQVGDKLYYV
                     HLKNSVGLRDGSRFPTGLGEGEINHREYIKLLKTTGYKGPVCIEAPRGGDREWYAKQD
                     IAYIKSVISDL"
     gene            complement(1409..2200)
                     /locus_tag="PK733_12560"
     CDS             complement(1409..2200)
                     /locus_tag="PK733_12560"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOJ11407.1"
                     /translation="MKFMIGVDLEGVACVVGEQNQPLSRANTNYDFACQQAVREVNAA
                     VKALFESGATEVYVWDNHGGSLNLPYDKLDERCQIVLGTGFATRWPGMDSSFTGALLI
                     GYHPMDNTENGILCHTFNSTNYQWIKINGEEVGEIAIDAALANELADVPVIFVSSDYK
                     GTQEANKVLPWAETVYTKTGLARNGAISKHPLRAAQDIYEGVLRAAARIDEMKTFSFG
                     SPIVYQCRYKRMENAQNASRERSKGSSLVDAYTVERVVERITDIY"
     gene            complement(2351..2426)
                     /locus_tag="PK733_12565"
     tRNA            complement(2351..2426)
                     /locus_tag="PK733_12565"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2391..2393),aa:Lys,seq:ttt)
     gene            complement(2433..2508)
                     /locus_tag="PK733_12570"
     tRNA            complement(2433..2508)
                     /locus_tag="PK733_12570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2474..2476),aa:Gln,seq:ttg)
     gene            complement(2862..3470)
                     /locus_tag="PK733_12575"
     CDS             complement(2862..3470)
                     /locus_tag="PK733_12575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOJ11408.1"
                     /translation="MIIRRAKIDDAYEAAELICMAWQESAYVLAGSSNKSEVMGVIEN
                     FFKQPKNSLSYQYIDVAEGKNGIAGLILSFPWDFTPRLNKPIIEELPDIYRSSQLDFK
                     EKVIPMIKTKEAKPDEYYVDSVAVYPKYRGHGIADGLLKVAKIKSATYGFDKMSLIVK
                     PENKRAISLYKKHGYTVRGKLTLGDKIYLSMVKSIDSEKALA"
     gene            complement(3797..3958)
                     /locus_tag="PK733_12580"
     CDS             complement(3797..3958)
                     /locus_tag="PK733_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11409.1"
                     /translation="MLKYLGLVGLAIALVLFAIYIRVHMEIANYIGNIFRKFIKYLIS
                     LLQNRKINN"
     gene            complement(4288..5385)
                     /locus_tag="PK733_12585"
     CDS             complement(4288..5385)
                     /locus_tag="PK733_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOJ11410.1"
                     /translation="MNKEIITTRQSISLLTIFMIGAAIILSPGKEAGKDIWIAILFAG
                     LLGLPMIFVHTRLLRIFPGDDLFDIQQKAFGKVIGKVISCFYIWFAFHLGSLVLRNFS
                     EFIQVVSLQETPQFPVLIIMVILCIWAVKSGIEVLGRWAAFFLPLFLFIVIGLTFLSI
                     PDMDLDNIRPVLYDGISPVLNGTASLFAFPFAEPVLFTMVFNSLKAKDKTLKAYIISM
                     LITGGVLITVSVRNILVLGLQNFTIIFFPSYSSASIITIGDYFGRIEALSGTIFFFAE
                     FVKASVSLYIASKGFAKILNFSHYNQLAAPIGLLMVCMATFVYSSTMEMFDWASEIYK
                     YYVLPFYVIIPLITLIVIEIKVRRNKSSNKQ"
     gene            complement(5471..5683)
                     /locus_tag="PK733_12590"
     CDS             complement(5471..5683)
                     /locus_tag="PK733_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11411.1"
                     /translation="MKIVIFSFVCAYYIFTDLIPIYNAKQRKIFWSYLAMISIAYCIN
                     LLVSMDVKIPSPAMLIRKAVTAIFKL"
     gene            complement(5700..6857)
                     /locus_tag="PK733_12595"
     CDS             complement(5700..6857)
                     /locus_tag="PK733_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ11412.1"
                     /translation="MKKNSIILKCIMAVMLSISLTGCWNYREIDQLYMVSGFAVDKSP
                     DGRYLITAETVDIKSGDKEATFASKTFSAEGATILDAIRNIVIISGRKLYFSHAKVAV
                     ISQDVAIEGIIQILDLIIRDTEPRLELNVMISKEKTAKELFITKNESTKIQGLEIDKM
                     VKSQISLSKAPTTKVYELINSLSTENESAVLPAVGFDMNAGIKDIALSGAALIKKDKF
                     VGFLDKEDTKHILFVKNQVKKGVLPEIESNEASHSSISLEIFESKTKITPVYSSGKTS
                     INVNIKTNAIVDEHGPVKSYIDKESISILKREAEKALEKNIKDTIHKVQEQYEVDIFG
                     FGNEIKRKMPALWKSIAQDWDNLFKDLDVDVKASIDIRGSGQISRPIKIGE"
     gene            complement(6862..8394)
                     /locus_tag="PK733_12600"
     CDS             complement(6862..8394)
                     /locus_tag="PK733_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ11413.1"
                     /translation="MFNLRNKINTQNNTRNSSKNNKEELLQASLEKNIKILKEIFIND
                     DTIIYRRFQNQCNNAIRCCIIFVDGMVNNEIVNKNIIQPIVINALLAGGKDIISNLQY
                     GIVAVNNVEKTADVYKVVESIINGDTALYVDGEKEALIISTKGWQNRQITEPETERVL
                     RGPREGFTESLMINLTMIRRKLKTPDLKFRFKTFGVRSRTKACICYIDGIANEEILKE
                     FNKRLDDIEIDGVLDTGYIQEFIADAPLSMFKTIGSTERPDIVAAKLLEGRIALVLDG
                     TPSALTIPFLFMEFLQSNEDYYVNYYTASIGRFLRNISLLLTISIPAIYLALVTFHQE
                     MIPTNLLMSISAARHGMPFPTVIEALAMLIVFEILREAGARMPTFIGQSLSIVGALVI
                     GQAAVQARFISAPMIIVIGFSGITSLVIAKLKGAVFLLRLIFLFLASIFGLYGYIFGI
                     IGLLIHLFEIRSFGVPYMSNLTSLNPQDLKDTAIRAPWWYMEYRPKFIAAKNIIRKKP
                     GT"
     gene            complement(8645..9415)
                     /gene="trpA"
                     /locus_tag="PK733_12605"
     CDS             complement(8645..9415)
                     /gene="trpA"
                     /locus_tag="PK733_12605"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOJ11414.1"
                     /translation="MNKLKQVFAKGKAFIPFITAGDPSLEITEQLVLQMADAGADLIE
                     LGIPFSDPVAEGPVVQEADNRALLGGTTTDKIFSMMARIRKSCNIPVAFMTYMNPIFT
                     YGTDRFMKNCQQVGVDAVIVPDVPYEEKDELMPFCSKYNVSLISMIAQASSNRIHMIA
                     SEAQGFMYCASSMGVTGAPQDINSDVKGMIKLVKEVRNIPCVICFDIYTPEQAAKIAE
                     FSDGVIVENAIVKIVEQYGVDCIPYVVEYVKRMIPALK"
     gene            complement(9417..10601)
                     /gene="trpB"
                     /locus_tag="PK733_12610"
     CDS             complement(9417..10601)
                     /gene="trpB"
                     /locus_tag="PK733_12610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOJ11415.1"
                     /translation="MSKGRFGVHGGQYIPEMLMNAVIELEEAYNHFKNDPKFIAELDD
                     LLKNYAGRPSLLYYAEKMTKNLGGAKIYLKREDLNHTGSHKINNVLGQVLLAKRMGKT
                     RIIAETGAGQHGVATATAAALMGMECEIYMGKEDTERQALNVYRMELLGAKVHAVTSG
                     TQTLKDAVNETMREWAQRVADTHYVLGSVMGPHPFPTIVRDFQSIIGREVKKQMLEKE
                     GRLPDVLLACVGGGSNAMGLFYDFIEDSNVRLIGCEAAGHGVNTDKNAATMANGTLGI
                     FHGMKSYFCQDKYGQIAPVYSISAGLDYPGVGPEHANLRDMGRAEYAAITDDEAVEAF
                     EYLSRVEGIIPAIESAHAVAYAKKIVPNMSKDQIIVINLSGRGDKDVAAIARYRGVQI
                     HE"
     misc_binding    complement(10750..11038)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3295 a         2386 c         1643 g         3773 t
ORIGIN      
        1 agtgccatat cagctattcg actcgcttct gcaaatgcca tgtccaaata agcctgatca
       61 gcattacttg atgcccgatt tcccgcccac acccttatta caggggcttt tagttcaatg
      121 gcagtttcca gcactctttc aaaaggcagc ttcttttctt cttcacaacc taaataataa
      181 tatgaaccat atgctgcaac acttaaacca gcatcttgtg tcatttgggc tacttcctct
      241 gcctttttag tatcgccatg gggaacatga atatcaccgc cccattcaat accatccaga
      301 gctgcttcag aaaccagctt aattatgtcc ccaggagaaa gctttctaaa agtaactgaa
      361 actaatcctg tttttaccat agttatcctc cttctactca ttctaattat aatatcacaa
      421 atcgctaatt actgatttaa tatatgcaat atcctgtttt gcataccatt ctctgtcacc
      481 gcctcttggt gcttcgatac atacagggcc cttataacca gttgttttaa gaagctttat
      541 atattctcta tggttgatct ctccttctcc caatcctgta gggaacctgc ttccgtctct
      601 aagtcctaca gaatttttta gatgtacgta ataaagcttg tcgccaacct gattaatagc
      661 ttctttcaaa gacggtatat tcttgaaata tactgtgttg ccgtaatcca ggttaactcc
      721 tacagccggg cttccaatca tatcaaccag tttccttgca gcagcaggca gatcatgtac
      781 gtagttcata tgagcttcaa atgcaaatct gaaacctaat tcttctgcaa gtttgccaag
      841 cactttaaat ccttctgcag cccatttcca ctgttcttct gcagctgcaa aagaaccatg
      901 tttatcgtat tcgctatagg gtatactttt atcagagtta aataatcccc ctgccattgt
      961 attgcataca gataagttaa accggcggga agcaagctta aagaaatttg tcatttcttc
     1021 aatttcgact tctcttttat tagaatcagg atctgataaa ttagcaccag ggcctccaaa
     1081 aataactgct tttaatcctg tttttcttac tcccttttct atagaatcaa gatattcctc
     1141 tacactttca ttaactccag aacgttttct tctgaattca acaccatcaa agccccaggc
     1201 aacagccttc tgacatattt cttcaacagt ctgtccctgt tcacaatagt ttacgtgcat
     1261 aattatatcc catgacataa ggttaatccc ctttcataca aaagtttttg ttctatatat
     1321 attttatcac aattgttctg acatgaaaac gtgcattcct gtaatcgggt atgcaaaaag
     1381 tataatgcat atcctcaatc tttaattctt aataaatatc tgttattctc tctacaaccc
     1441 tttcaactgt atatgcatcc accaggctgg atccttttga acgttctctg gatgcattct
     1501 gcgcattctc catacgtttg taacgacatt gatatacaat gggactgcca aagctgaagg
     1561 ttttcatctc atcaatcctt gctgctgccc ttaaaacacc ctcataaata tcttgagcag
     1621 ctcgcagcgg gtgtttactt atagctccat ttcttgccaa tcctgttttt gtatacactg
     1681 tctctgccca gggcagtact ttgtttgctt cttgagttcc tttatagtca ctggatacaa
     1741 agattacagg gacatctgcc agctcatttg ccagagctgc atctatagca atttccccaa
     1801 cctcttcacc attaattttt atccactggt aatttgtaga gttaaatgta tggcatagaa
     1861 tcccattttc agtattatcc atgggatgat atcctataag caacgcacct gtaaagctgc
     1921 tatccatacc gggccaacga gttgcaaaac cagtacctaa tacaatctga cacctttcat
     1981 ccagtttatc atatggaaga ttaagacttc ctccatgatt atcccataca tagacttctg
     2041 tagcacctga ttcaaaaaga gctttaacag cagcatttac ttcccgtacg gcttgctgac
     2101 atgcaaaatc ataatttgta ttagctctgc tcaatggctg attctgctct cctacaacac
     2161 atgcaacacc ttccaggtca acacctatca tgaatttcat aatacttatc accctcctgc
     2221 tttttcgata acttcaataa ttcagagtta tctcacttgt ggctcactca atattatagc
     2281 acttaatatg ctgtagtgga aataaaaaaa cgtacagaat tttgtattat caaaatatct
     2341 gtacgttttt tggtgatcca tccgcgactc gaacgcggga caccttgatt aaaagtcaag
     2401 tgctctgcca actgagctaa tggaccatat actggctggg atggcaggat ttgaacctac
     2461 gaactgccag agtcaaagtc tggtgcctta ccacttggct acatcccatc gtttagctgc
     2521 gaattctatt gtaaaggaca atatatgatt tgtcaagaga aaattttttt aattttttaa
     2581 tttttacgta attactttat tatagtcata gtttttatag tctttgccaa aagccccttt
     2641 attaattcat agattcatat gtagtttttt caagtattat ggtaacagga ataaaaccct
     2701 atattccacc gctgctggtc caaactagtc tcctgcaaaa gcagtgaaaa gaaccatttt
     2761 caaaatggct tcactgcttg caatgtagtc caatatacta aggttatttg tatatcaata
     2821 actatctaag catctaatgt cgtgcttagc acttacttag ctcatgccaa cgctttttct
     2881 gaatcaattg actttaccat gcttaaatat attttgtctc ccaaggttaa tttccctctt
     2941 acagtataac catgcttctt atataacgaa attgctctct tattttccgg cttaactatt
     3001 agagacatct tgtcaaaacc ataagttgca gattttattt tagcaacctt aagtagccca
     3061 tctgctatac catgtcctct gtatttggga tatacagcaa cagaatctac ataatattca
     3121 tcaggctttg cttctttggt cttaatcata ggtattactt tctctttgaa atctaattga
     3181 ctagacctat aaatatcagg tagttcctct ataataggct tgttaagtct cggagtaaaa
     3241 tcccaaggga acgataaaat aagacctgct ataccgtttt taccttcagc cacatctata
     3301 tattgataac tcaaagaatt tttaggctgt ttaaagaaat tttcgatcac tcccattact
     3361 tcagatttat tgctgcttcc cgccagcaca tatgcgcttt cctgccacgc catgcatatc
     3421 agttctgccg cctcataagc atcatcaatt ttcgctctgc ggataatcat atatatcatt
     3481 cctccataag taaataatta ttataggtat actttataga aaactataac agcataccaa
     3541 attagtacca taataatgcc ataatgatat tattaagata catttatgtt taaaaaaatg
     3601 tatctacttt taaactaaaa taatagacaa ttttccacga acctttattt agaaaatata
     3661 attgtttcta ttccgtataa gtattagtaa agtagagttt tttttgcaca attacattat
     3721 accacaaaag agaacattat caaaatagga gaaccctttc aaactttcaa tgtacacata
     3781 gaaataattg ataagctcaa ttgtttattt ttctattttg aagtaagctt attaagtatt
     3841 ttataaattt tctaaatata ttaccaatat aatttgctat ctccatatgc actcttatgt
     3901 atattgcgaa taaaactaaa gctattgcta gacctactaa acctaaatat tttaacatga
     3961 tatcatcctc ctatagcaag ttttaataaa ttaaatcaca ttaattaact tatatgacta
     4021 ggatgctatt aggttatatg gaattattat atatctgata ttatatatta tatttccatt
     4081 actttcaagt tatatcatat cgaaattact gctatatcgt agtgtagcgt aattgctgtc
     4141 tttgaacgat ttatagtaat gttattgata ctcttttttt ttcctataac tctgtaaaaa
     4201 cattggagct tttcagctaa tatcgtaagc gggctgatcc ggtcgttttg gagaaaaagt
     4261 ctcaattact tccgttttct caatatttca ttgtttattg ctagatttat tacgcctcac
     4321 tttaatctct attactatta atgtaatcaa aggaatgata acataaaaag gaagtacata
     4381 gtacttataa atttcagatg cccagtcaaa catttccata gtacttgaat atacgaaggt
     4441 tgccatacat accatcaaaa gtcctattgg agctgcaagc tgattataat gactaaagtt
     4501 tagtatttta gcaaagcctt tactcgctat ataaagacta acactcgcct ttacaaattc
     4561 tgcgaaaaaa aatattgtac ccgaaagtgc ttctatcctt ccaaagtaat cacctatagt
     4621 tattatgctt gcagaagaat aagaagggaa aaatatgatg gtaaaatttt gcaaacctag
     4681 gactaaaata tttctaacgc ttactgttat taaaacacca ccagttatca gcatactaat
     4741 aatatacgct ttgagggttt tatctttagc ctttaatgaa ttaaaaacca ttgtaaacaa
     4801 tacaggctct gcaaatggaa aggcaaataa agatgccgta ccattcaaaa ccgggcttat
     4861 accatcgtac aacacaggcc ttatattatc tagatccata tctggtattg ataaaaaagt
     4921 taaacctata actataaata aaaacagtgg aagaaaaaat gctgcccaac gccccaatac
     4981 ctcaataccg ctctttacag cccatataca taaaattacc attattatta aaacaggaaa
     5041 ctgaggcgtt tcctgcaaag atactacttg tataaactct gaaaaatttc tgagtaccaa
     5101 agaaccaagg tgaaacgcaa accatatata aaagcacgat attactttac caatcacttt
     5161 accgaaagct ttctgctgta tatcaaataa atcatccccg gggaaaatcc taagaagtct
     5221 tgtatgaaca aaaatcatgg gcaatccaag aagtcctgca aataaaattg cgatccaaat
     5281 atcctttccg gcctcttttc cggggctcaa tattatagcg gcacctatca taaaaattgt
     5341 caacagactt atgctctgtc tggttgttat aatttcctta ttcatctttc gcctgcttcc
     5401 acttgttaat aaaagtattt attaattggc tttattgatt atatttatag tttatattta
     5461 ttgtttactt ttataatttg aaaattgctg taactgcttt tcttattaac atagcaggac
     5521 tgggtatctt gacatccatt gaaactaaca ggtttataca atatgcaata gatatcattg
     5581 ctaaatatga ccagaatatt ttacgctgct ttgcattata aatcgggatc aagtcagtga
     5641 atatataata ggcacataca aatgagaata ttacaatttt catacgctat tctccttttt
     5701 tattctccaa ttttaattgg tcttgatatt tgtccagagc ctctgatatc tattgatgct
     5761 tttacatcca catccaggtc tttaaaaaga ttatcccaat cttgcgctat gcttttccac
     5821 aaagcaggca tttttctttt aatctcgttt ccaaatccga atatgtcaac ctcatattgt
     5881 tcctgaacct tgtgtattgt atctttgatg tttttctcca gggctttttc agcttctctt
     5941 tttagtatgc ttatgctttc cttgtcaata taacttttaa caggaccatg ctcatcaaca
     6001 atagcatttg tttttatatt aacatttatt gaagttttgc cactagagta aaccggtgtt
     6061 attttagttt tactctcaaa tatctccaat gatatactgc tatgtgaagc ttcattcgac
     6121 tctatctcag gaagcacacc tttttttacc tgatttttta caaaaagaat gtgcttggta
     6181 tcttctttgt caagaaatcc aacaaactta tcttttttaa tcaatgccgc tcctgataat
     6241 gctatgtctt ttatacctgc attcatatca aagccaacag cgggaagaac tgccgactca
     6301 ttttctgtgg ataatgagtt gattagctca taaacttttg tagttggcgc cttagatagg
     6361 cttatctgag actttaccat tttatctatt tcaagtccct gtatttttgt tgattcattc
     6421 ttggtaataa ataattcctt ggctgttttc tcttttgaaa tcatcacatt aagctccagc
     6481 cttggttctg tgtccctgat aatcaagtca agtatttgaa ttattccttc tattgctaca
     6541 tcctggctta tgactgcaac ttttgcatgg ctaaaataca attttcttcc agaaataatt
     6601 actatatttc ttattgcatc aagaattgtc gctccctctg ctgaaaaggt ttttgaggcg
     6661 aatgtagctt ccttatctcc tgattttata tcaactgttt cagcagttat caaatatctg
     6721 ccgtctgggc ttttgtctac tgcaaaaccc gataccatat atagctggtc gatttcccta
     6781 tagttccagc aacctgttaa tgatatcgaa agcatgacag ccataatgca ttttagtata
     6841 atactatttt ttttcataaa ctcatgtccc cggctttttc cttattatat ttttggcagc
     6901 aataaacttg ggcctatatt ccatgtacca ccatggggct cttatagcag tgtccttaag
     6961 atcctgagga ttcaatgatg tcaaattgga catatagggt accccgaagg aacgtatttc
     7021 aaacaagtgt atcaacaatc ctattatgcc gaaaatgtaa ccgtataatc cgaaaataga
     7081 ggcgagaaat aaaaaaatta atcttagtaa aaaaactgct cctttcaact ttgctatcac
     7141 aaggcttgtt atgccagaaa agccaatcac aataatcatt ggagcactta taaatctggc
     7201 ctgaactgct gcttgcccta taaccaaagc tcctacaata ctcaaggact gccctatgaa
     7261 tgtgggcata cgcgctcccg cttccctcaa tatttcaaaa acaatcaaca ttgctaatgc
     7321 ttcaataacc gttggaaagg gcatgccatg tcttgcagct gatatactca taagaaggtt
     7381 ggtaggtatc atctcctggt ggaaagttac taatgccagg taaatagcag gtatacttat
     7441 agttaataat aaacttatgt ttctgaggaa ccttccgatt gatgcagtat agtagttgac
     7501 atagtagtct tcatttgatt gcaaaaattc catgaataaa aaaggtattg ttaacgccga
     7561 aggagtacca tcaagaacaa gggctatgcg gccttccagc agcttagctg ctacaatatc
     7621 cggccgttca gtactaccta tggttttaaa cattgaaaga ggtgcatctg ctataaactc
     7681 ctggatataa cctgtatcta aaaccccatc aatttcaatg tcatccagcc tcttgttaaa
     7741 ctctttcaga atctcttcat ttgctattcc atcaatataa caaatacatg ccttggttcg
     7801 agaccttaca ccaaaggtct taaacctaaa ttttaaatcc ggagtcttta attttctcct
     7861 aatcatggta aggtttatca tcaaagactc cgtaaaccct tctcgtggtc cccgtagaac
     7921 cctttcagtt tccggttcag taatttgacg gttctgccaa cccttggtgc ttattattaa
     7981 tgcttctttt tcaccgtcaa cataaagtgc agtatcacca ttaattatgg attcgacgac
     8041 tttatacaca tcagcagtct tttctacgtt atttactgca acaataccat attgcaaatt
     8101 gctgataata tcttttccac ctgctaaaag tgcatttatt acaataggct gaataatatt
     8161 cttattaact atttcattgt ttaccattcc atcaacaaaa ataatacagc accttattgc
     8221 attattgcat tgattctgga accttctata aataatagta tcatcattga tgaatatttc
     8281 tttcaatatt ttaatatttt tctccaacga tgcttgaagc aactcttctt tattgttttt
     8341 agatgaattt cgcgtattat tctgagtatt tatcttattt cgcaagttaa acataatgca
     8401 ttccttatca taatatttat aagagtaatt attcattata actattattt gaattactca
     8461 ttaattttat tccaacgctc atttctttgt cgtcggcaaa tgacaaggtg ttcccaattt
     8521 ccagtttatg aactttataa atgtaactta agtgtaacct aatagggaca gtcactcatt
     8581 taataacgaa actcaataac cacccctatg tttcacatat ttcacatatt tcacataccc
     8641 tatttcattt gagggctggt atcattcttt tcacatattc aactacataa ggtatacaat
     8701 ctacaccata ttgctccacg attttcacaa ttgcattttc aactattaca ccatcggaaa
     8761 actcagctat ctttgctgct tgttcaggtg tataaatgtc aaagcaaatt acacaaggga
     8821 tgttcctgac ctcttttaca agttttatca ttcccttcac atcgctgttg atgtcttgtg
     8881 gcgccccggt cactcccata gatgatgcac agtacataaa tccttgcgct tcactggcaa
     8941 tcatatgtat gcgattacta gatgcctgag ctatcattga tatcaggcta acattgtatt
     9001 ttgagcaaaa tggcattagt tcgtcttttt cttcatatgg tacatcagga acaattactg
     9061 cgtctactcc gacctgctgg caatttttca tgaatctgtc agtgccataa gtaaatattg
     9121 gattcatata agtcataaac gccacaggga tattgcatga ttttcgaata cgtgccatca
     9181 tgctaaatat tttatcagtg gttgtcccac ctaaaagagc acgattatct gcttcttgta
     9241 ccacaggtcc ttctgcaacc gggtcagaaa acggaattcc caattcaatt aaatctgcac
     9301 cggcatctgc catttgaagc actaactgct cagttatttc taatgaagga tctcctgcag
     9361 tgataaatgg tataaacgcc ttaccctttg caaatacttg ctttaactta ttcatgttat
     9421 tcatgaattt gcacccccct gtaacgtgct attgcagcaa catccttatc tcctctgcca
     9481 gaaagattga ttacaataat ttgatcctta ctcatatttg gtacaatctt ttttgcatat
     9541 gcaactgcat gtgcactctc aattgccggt ataattcctt ctacgcgtga taaatactca
     9601 aatgcctcta cagcttcgtc gtcagtgatg gctgcatatt ccgccctgcc catatcacgc
     9661 aggttagcat gttcaggtcc tacgccggga tagtcaagcc ctgctgaaat ggaatatact
     9721 ggtgcaatct gtccgtactt atcctgacag aagtatgatt tcatgccatg gaagatacct
     9781 aaagtcccgt ttgccattgt tgcagcattc ttgtctgtat taacaccatg cccggctgcc
     9841 tcacatccaa taagccgaac gtttgaatcc tctataaaat cataaaacaa tcccattgca
     9901 ttgctgccgc ctccaacaca tgccagcagg acatcaggta gtctgccttc cttttccagc
     9961 atctgctttt taacttcgcg accaattata ctttgaaaat cacggacaat ggttggaaaa
    10021 ggatgcggac ccattacaga tccaaggaca tagtgagtat ctgcaacacg ctgtgcccac
    10081 tcccgcatag tttcgttgac tgcatcttta agagtttgag tgccgctggt tactgcatgt
    10141 acttttgcac ccagcagttc catgcggtaa acattgagtg cctgacgttc ggtgtcttcc
    10201 ttgcccatgt aaatttcaca ttccattccc ataagtgctg ctgcagttgc tgtagcaacg
    10261 ccatgttgtc ctgcgccagt ctctgcaatt atacgggttt tccccatacg tttggcaagg
    10321 agcacttgcc ccagtacatt gttgatttta tgagagcccg tgtgattgag gtcttcacgc
    10381 ttgagataaa tcttagctcc acccagattt ttcgtcatct tttcagcgta ataaagcaat
    10441 gacggacgtc ctgcatagtt tttaagcaga tcatctaatt ctgctataaa tttcggatca
    10501 ttcttaaagt ggttatatgc ttcttcgagc tcaattacag cattcatgag catttccggt
    10561 atatattgcc ctccatggac cccaaatctt ccttttgaca tcttcatcat tctccttatt
    10621 tacattagtt tttgcaaaaa tttattgcca cgcgattatg caatgttatc atgtaatcaa
    10681 acaacattat catgtaaata aacaatgtta tcctataatt ttacaaataa aaaaagcctt
    10741 tatccctgta atagggacaa aagctttagc ttctgcggta ccacccaaat tgacgataaa
    10801 tcgtccactc attatgtata ccatcatata cacccaactt ataacgggca ggatccccgt
    10861 aagcgcctac tttactctaa atttcgtttt actcagtcta ctcaatctac tcagtacttt
    10921 tcaatttcgg gcttccctca caagtccatt cagaataatc tttgtaaccg cattctcacc
    10981 accggcggct ctctttgtac aagtaataat tcttactctt cttgctcata ggtttatcat
    11041 attaaataaa attgtagcat tggcaagtgc tcttgtcaac tataattttt gctaatt
//
LOCUS       DAOSDD010000145        11055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2270_length_11055_cov_5.000455, whole genome shotgun sequence.
ACCESSION   DAOSDD010000145 DAOSDD010000000
VERSION     DAOSDD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2270_length_11055_cov_5.000455"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PK733_12615"
     CDS             <1..537
                     /locus_tag="PK733_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11416.1"
                     /translation="MGIEGYGMYMLLSVAAPELAGRWGIAPIPGTRKKDGTIDRSSGG
                     VAGEAIVIMNQSPKKNESWEFLKWWTSTEVQAQFGKELEALVGPQARWNTANTEAFLS
                     LPWDSKDLIVLREQWQWYKEMPVVLGGYFTGRHIANAWNRIIIGEMPVRDSVEKAVKD
                     INRELKAKQQEYGIFIDE"
     gene            549..1538
                     /locus_tag="PK733_12620"
     CDS             549..1538
                     /locus_tag="PK733_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ11417.1"
                     /translation="MAELTKITKPDTNVDLNMKKEYNRKTQPSIKNNSVLKSWWAKYG
                     TGYLYLAPFLILFIIFIIIPVFAAIGLSFTNYNMLQAPKWLGINNYRLLIMDDDIFLL
                     SLKITMIFAAITGPIGYIMSFIAAWVINQLKFKNAFALAFYAPSLTSGIAMSVVWLYF
                     FSSDRYGLINYLLLNLGLIVKPILWTLDAKTILPIITVISIWMSMGTGFLVFLAGLQN
                     VPRELYEAGCIDGIRTKFQELFYITLPMLKPQMLFGAINTTVGALGVFDISVSVAGMP
                     SPNYAGHTIVAHLYDYAFIRFQMGYASTIAVILFAITFILGRIFMKVFSSKDM"
     gene            1554..2441
                     /locus_tag="PK733_12625"
     CDS             1554..2441
                     /locus_tag="PK733_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11418.1"
                     /translation="MAVVKSGVRIKVSKFRVRLSFGSILLNIFMILLAAFTAIPLVYM
                     ISTAFKPLDELFIYPPRYFVRRPTLSNFTDLLISLGSSVVPFSRYIFNSLFTATVTVA
                     GTVVVSGMGAYALSKHKLPGAGFIFSVIIAALMFSSHVTQIPSYIVVDKLGLLNSYWA
                     LIIPKLAVAYNFFLMKQFLDQFPNELLEASRIDGAKEHTIFWKIVMPSLRPAWSTLVV
                     FSFVANWNDYFSPLVFISSQAMKTLPLALQTIGAAGNIARAGAMNAATLLMTLPTLII
                     FLVMQAMVMETMAHSGIKA"
     gene            2471..3913
                     /locus_tag="PK733_12630"
     CDS             2471..3913
                     /locus_tag="PK733_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11419.1"
                     /translation="MSSVKKIVFIIIVLIIAVQIMPINILASSHYVLDTKDPKIKVPL
                     PLTYEVKRVISEISDEISLTNGPEDLFIDNNNMLYVVDTGNNRIVKMDREGMLIGIFK
                     GPEGKELVNPKGIYVDTDGDMYIADTGNYRILHLSPEGDFVEEFSKPNTELIGETFTF
                     DPEKISIDSIGNIYIIKGYTLAILDAYNNFRGFKMSEKLAFNFTEALVNLFASREQKE
                     RVARRAPSPYSNFVIDNRGMIYATAINTYQRQIQKINSVGNNIYPSKAYGEMIIDERG
                     LARTPYFVDLSVDKNGIISAIEQQSGKIYQYDQEGNLLTVFGGKGYWKGSFALPSSIT
                     VDDEGNIYVLDRTLNNIQVLEPTLFMRLIHEASMEYEEGRYQESLELWEQVLRINENY
                     QLAHKGKAKALAKQERWKEAMKEYSAADDQEGYSNAFSEYRYYLFRQYFGWILLIIIL
                     INVAIYNIIKFSKKIINNMVDGFIYGKGLK"
     gene            3910..4536
                     /locus_tag="PK733_12635"
     CDS             3910..4536
                     /locus_tag="PK733_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11420.1"
                     /translation="MMEALKFSISVLFRPLDAFNYIKINRGDIQYWAPACLVFLMLPI
                     RVLSIFLEHYPVAVLLPNETNLILEAVKVLVPVLTWTISCFAVTSIAGGETTQKEIFI
                     ASAYSIVPYLIITIPQSIFSKLLGRSELGLYNTLETIKWVWVVLLFIINVKVMNDYSI
                     KKAIAICALGIVGVVLIWGVLLLAYVLTNQLYSFLASVILEIRMLYGG"
     gene            4552..6801
                     /locus_tag="PK733_12640"
     CDS             4552..6801
                     /locus_tag="PK733_12640"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ11421.1"
                     /translation="MKNRIIIILICVILLLASTIYVVAEKDITLEKQKDSTVEKDSTV
                     ETGLISTESDSQPSMSLRPGFEKIAETEALELYYNEEVISILVKDKRNGFLWSSNIEG
                     GYYNMEETNEFWKENMSSLFYISYTSDYKKINSTNSVIKESVIKSNPVENGVAIEYDL
                     TRLGIKLTLEIWLDNKFLNVKIPVEKIEEYGNNYITSIELFPFFGAASDYEKGYVFYP
                     DGSGGLYHFKGKPEIAAEKYSWPIYGYDKLNLDEIEKREKAELKQALLPVFGMKKGDN
                     AFVGIVTKGEYDTSINFYPSGYAVNLSRVNIEFFYRRLHKDLRPYATVTDYIEKGRIN
                     DDHEIKYNFLVNENADYSGMASAYREYLESIDAIQYTVSPNEDIPFWLDLFMGIKLDQ
                     VLFDKYIKMTTFEEVEKILENFKNKGVNNIMANLIGWVKGGYGNFPANWPPEKALGGT
                     KGIIKLANYASNNNMKLFLENNLVDALAKNRGFSKRNDVVYHRNGLSVTDEKREKYIL
                     NPVKVFERFIESLIPNIKKVHISGLSFDRLGSFIYYDYHKNNPVTRCETAELWTRIME
                     ETRIEIGSTAVRGGDAYTLKYADWLLDIPVEDSGYFITDEAVPFYQMVVHGFIPYSSE
                     AGNLFHDFQRQKLKWIEYGCMPYFKFTYQEPVLIRKTEYNHLFTSYYLHWIDIAVDIY
                     QEFNEKIGELWSVNIIKHEKVLENVYKVTYKNGSKIYINYNEDSVYIDNFSIKGMDYI
                     VVDKGGTKK"
     gene            6798..7715
                     /locus_tag="PK733_12645"
     CDS             6798..7715
                     /locus_tag="PK733_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ11422.1"
                     /translation="MKKFRLKMKYKHMLEGYILLLPWIIGTVLFFIVPIYQSIFLSFS
                     KLVKITEFKMKWVGLIHYQRALFFDIWFVPVFISMIKETLTNTPLIIIFSLFIAMLLN
                     KNTKFKGFFRGVYVLPIVLGTGYVMSQLLGIRVISSPEGAPPPMVVSGELSRGLLIPE
                     EMLIYLGPTITKAVTLFLSKITMMLWKSGVQIVLFLGGLQSISKSLYEAAHCDGATEW
                     EMFWKITFPIISPTILLSIVYTIADSFTDASNPMVQKVMQLGFNTVQFEYAAAISWIY
                     LAFIMLLIGLVFLVFRIGQNSIESKKVEV"
     gene            7717..8730
                     /locus_tag="PK733_12650"
     CDS             7717..8730
                     /locus_tag="PK733_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11423.1"
                     /translation="MVMMGKVFKKVLSPNEKDMYWSKKGGLKNEQDNPKLRKLKQLSF
                     WRRNFYKTANYLILADLAFVFLLPFLYMIITSLKTDADLVDTTVKWIPTTICIENYVR
                     AFRALDYVKHLKNSVLVTGLCTAGHVFSCSLAGYSLAKYRYYGRNLFFTFVILSIITP
                     IQVILLPLYLRYGKLNMLGTYLPLILPTFFGYGLSGALFIFIYRQYFLGIPQEFIEAA
                     KIDGCGFISTFYRVVFPTSKSPTLVTTVLSLIWHWNDYFEPTFYIRRSHLHLLSQKLP
                     NIYHLFQQAGKFDPVTQLQIKVYNEAVFMAAIFLVVLPLLVIYIVLQRQFVEGIERTG
                     HVE"
     gene            8849..>9083
                     /locus_tag="PK733_12655"
     CDS             8849..>9083
                     /locus_tag="PK733_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11424.1"
                     /translation="MRKINITTTNPYTVKPVEPEIPVFTKEEFEDRISRLLNKMSNSE
                     LTHVIVYGDREHFSNLEYLTGFDPRYEEGMLIIQ"
     assembly_gap    9084..9093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9094..10287
                     /locus_tag="PK733_12660"
     CDS             <9094..10287
                     /locus_tag="PK733_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11425.1"
                     /translation="QGDGKLTLLVGNEGWNYSFISPLNLERILYRNFSLQGQPRDNQP
                     CLKEVFKNCGIDKYSNIGVIGQKYFESDHIKGCEHEAGYFTDVPSYIIDILVQIVDRN
                     RITNFTHAMTNLSDGLRITVKGAKEIAYYEYVTNKVSNCVINMLEGLEPGISELEASR
                     KAGYDASPINMFPLVNFGEKHAFLCLRSPNHNKLKEGNLVTICYGIRGGLVARSGLAV
                     KDKSGISNSLGNIIEEFYMPYFRAVITWYESVKIGKNCGEVYNKVMDILGGEKDFGVS
                     LNPGHNISSDEWTNSPFYKDSPHKILNGYYLQCDIIASLQNPVRLAMLEDGVIFADEK
                     LREELKSQYPDVYNRIQKRREFMIEVIGINLSDDVLPLSNAQAICHPFLLDTNKIFTI
                     DKLMK"
     gene            10320..>11055
                     /locus_tag="PK733_12665"
     CDS             10320..>11055
                     /locus_tag="PK733_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ11426.1"
                     /translation="MSFKNESCFLELNLCDINSQLKSYIYKLSDEAFLLGDNARDSIN
                     SIGEFEKRRTYLREKFIESMGGLSSLETPLNPVVTGTIEGDGFRIEKVILESRPKTYV
                     TCNLYIPNNIDKPVGAVLLLCGHSQNGKEDYQIACQYFAKAGMVVLVQDPIGQSERLS
                     YYDAATGKINITWGTYEHNYAGSQSLFLGEGIARYFLHDSVRAIDYLCTRPEVDPTKI
                     GVTGCSGGGTQTCMLMLYDQRIAAAAP"
BASE COUNT         3793 a         1623 c         2231 g         3398 t
ORIGIN      
        1 atgggaattg aaggttatgg aatgtacatg cttttatcgg tagccgcccc ggaacttgcc
       61 gggagatggg gcatagcccc gattccggga acaagaaaaa aagatgggac cattgacaga
      121 tcaagcgggg gagtagctgg agaagcaatt gtaataatga accagtcccc aaaaaagaat
      181 gaatcatggg agttcttaaa atggtggacc agtacggagg ttcaagcgca gtttggaaaa
      241 gaacttgaag cactggtagg tcctcaggcg agatggaaca ctgctaatac agaggcattt
      301 ttatctctcc cgtgggattc aaaggacttg atagttctta gggaacagtg gcaatggtat
      361 aaggaaatgc ctgtggtgtt aggcggttac tttacaggac gtcacattgc caacgcatgg
      421 aatcgtatta taataggtga aatgcctgtc cgagattctg ttgaaaaggc tgttaaagac
      481 ataaacagag agctaaaggc caaacagcag gaatatggta tttttattga tgaatagggg
      541 tgaaggattt ggctgaatta actaaaataa caaaacccga tacaaatgta gacctcaata
      601 tgaaaaaaga atacaacagg aaaacgcaac ccagtattaa aaataacagt gtgttaaagt
      661 catggtgggc aaaatatggt acggggtatt tgtatcttgc accattctta atactattta
      721 taatttttat tataattcct gtatttgctg caataggatt aagctttacc aactacaata
      781 tgcttcaagc accaaaatgg ctgggtatta ataattatcg tcttcttata atggatgatg
      841 atatattcct tctgtcttta aaaattacaa tgatattcgc tgcaatcaca ggacccatag
      901 gatatattat gtcattcata gcagcatggg taataaacca gctaaaattc aagaatgcat
      961 ttgcccttgc gttctatgca ccttccctca caagcggtat agctatgtca gtagtttggt
     1021 tatacttttt ctcttcagac agatatggtt tgataaatta tttgcttctg aatcttggct
     1081 taatagtaaa acctattctc tggacattgg atgcaaaaac catacttccg ataataacag
     1141 taatatcaat atggatgagc atgggaacag gatttcttgt attcttggca ggtcttcaga
     1201 atgtgcccag ggaattatat gaagccggat gtattgatgg aataaggact aagtttcagg
     1261 agttattcta tataacgctt ccaatgttga aaccacagat gctttttggt gcaataaaca
     1321 caacagtagg tgctcttggt gtgtttgata tatcagtatc agttgccggg atgccaagtc
     1381 ccaactatgc agggcacaca atagttgcac atctttatga ttatgccttc ataagatttc
     1441 aaatgggata tgcttctaca atagccgtaa ttctatttgc aattacgttc atacttggaa
     1501 ggatattcat gaaggtattt tcatcaaaag atatgtgata ggaggatata aaaatggcag
     1561 tagttaaatc aggagtcagg ataaaagttt cgaagtttcg tgtaaggttg agttttggca
     1621 gtattcttct taatatattt atgatactat tggcagcatt tacggcaata ccgcttgtgt
     1681 atatgatatc tacagcattt aagcctcttg atgagctgtt tatatatccg cccagatatt
     1741 ttgtaagaag acctacactt agcaacttta cggatttatt aatatctctt ggaagctctg
     1801 ttgtaccttt cagtagatat atattcaaca gcctttttac tgcaactgta acagtggcag
     1861 gtactgtagt ggtatccggg atgggtgcat atgcactttc aaaacacaaa ctaccaggtg
     1921 ctgggtttat attctcagta attatagcgg cactgatgtt ttccagtcat gtaacccaga
     1981 taccatcata catagtggtt gataaactag gtcttcttaa ctcatattgg gcattaataa
     2041 tacctaaact tgcagtagcg tacaattttt tcctcatgaa gcaattcctg gaccagttcc
     2101 caaatgagct tcttgaagct tccaggatag atggtgccaa ggaacacaca atattctgga
     2161 aaatagtaat gccctcatta aggcctgcat ggtcgactct tgtggtcttt tcatttgtgg
     2221 caaactggaa tgattatttt tcaccattgg tatttataag cagtcaagca atgaaaacat
     2281 tgccccttgc actacagacc ataggggctg caggaaatat agcaagggca ggagctatga
     2341 atgctgcaac cttgctaatg acattaccta cgttaattat atttctcgta atgcaggcaa
     2401 tggtaatgga gacaatggca cattcaggaa taaaagcata aaaaccgtta tatgtaaagg
     2461 tggtatttaa gtgagtagcg taaaaaaaat agtatttatc ataattgttt taataattgc
     2521 agtacaaata atgcctatta atatcttggc tagtagtcac tatgtacttg atacaaagga
     2581 tcctaagata aaagtacccc tgccgttgac atatgaagtt aaaagagtaa taagtgaaat
     2641 atctgatgag ataagtttga cgaatggacc ggaagaccta tttattgaca ataataacat
     2701 gctttatgtt gttgatacag ggaacaatag aatcgtaaaa atggacagag aaggtatgct
     2761 tataggcatt ttcaaaggac ctgaaggaaa ggaacttgta aatccaaaag gtatatatgt
     2821 agatacagat ggagatatgt atattgcaga tacagggaat taccgcatac ttcatctttc
     2881 tccagagggg gattttgtag aagagttttc caaacccaac actgaactta ttggagaaac
     2941 ttttaccttt gatcctgaga aaatatctat agactccata ggaaatatct atataataaa
     3001 aggctacacc cttgcaatcc ttgatgcata caacaatttc agaggattta aaatgtcgga
     3061 aaaattagct tttaatttta cagaggctct ggtaaatctt tttgcttcta gagagcagaa
     3121 agaaagggta gcaaggagag caccttctcc atattcaaat tttgttattg ataatagggg
     3181 aatgatatac gccactgcta taaatacata tcaaagacaa atacaaaaga ttaactcagt
     3241 gggtaacaac atatatccga gcaaagctta tggtgagatg ataatagatg aaagaggctt
     3301 ggctcgtaca ccctattttg tcgacttatc agtagataaa aatggaataa taagtgctat
     3361 tgaacagcaa agcggaaaaa tatatcaata tgatcaagaa ggaaatctgc taactgtttt
     3421 tggcggaaaa ggatattgga aaggttcatt cgccctgcct tcaagcataa cagtagatga
     3481 tgaagggaat atctatgtgc ttgatagaac acttaataat atccaagtac tagaacctac
     3541 tctattcatg agattaattc atgaagcctc aatggaatat gaggaaggaa gatatcagga
     3601 atctcttgaa ctttgggagc aagttctaag aataaatgaa aattatcaac tagcgcataa
     3661 aggcaaggca aaagctttag caaaacaaga aagatggaag gaagctatga aagagtatag
     3721 tgcagctgat gatcaggaag gttattcaaa tgctttttcg gagtatagat actacctttt
     3781 taggcaatat tttggttgga tactgctaat aattatactt ataaatgttg caatatataa
     3841 tattattaag ttttcaaaaa agataataaa taacatggtg gatggattca tctatggaaa
     3901 ggggttgaaa tgatggaagc tttaaaattt tcaatatcag tattgtttag acctcttgat
     3961 gcctttaatt acataaagat aaacagaggc gacatacaat actgggcacc tgcttgttta
     4021 gtattcctaa tgcttccaat aagagtatta agtatttttt tagaacatta cccggtagca
     4081 gtcttattgc ctaatgaaac aaacctcatt ctagaagcag taaaagtact cgttccagta
     4141 cttacatgga caatatcatg ttttgctgtg acttctattg caggagggga gactactcag
     4201 aaagaaatat ttatagcatc tgcttattct atagttcctt atttaattat cacaattcct
     4261 cagagcattt tttcaaaatt gttggggcgt agtgaacttg ggttatataa cacactagaa
     4321 accataaaat gggtatgggt agtgctgctg tttattatca atgtaaaggt aatgaatgac
     4381 tacagtataa agaaagctat tgcaatatgt gcgctgggaa tagtcggtgt cgtactaata
     4441 tggggtgtgc tgctgctggc ttatgtactt accaaccagc tttattcatt tctagcaagt
     4501 gttatattag agatcaggat gttatatggt ggttaaggag gactatacct aatgaaaaat
     4561 cggattatta ttatattaat atgcgtaatt cttttgctag cgagtacgat atatgttgta
     4621 gctgaaaagg acattacctt ggaaaaacag aaagactcta ctgtagaaaa agactctact
     4681 gtagaaacag ggttgatttc gacggaatcg gattcacagc ctagcatgag tttgcgacct
     4741 gggtttgaaa aaatagcaga gactgaagcc cttgaacttt actataatga agaagtaatt
     4801 tcaatacttg ttaaagacaa aagaaatggg tttctttgga gttctaatat tgagggtggt
     4861 tactataata tggaggagac aaatgaattt tggaaagaaa acatgtcttc cctgttttat
     4921 ataagctata ccagcgacta taagaagata aactcgacaa acagtgttat aaaagagtct
     4981 gtaataaagt caaatcctgt agaaaatggt gttgctatcg aatacgattt gacaaggctt
     5041 ggtataaaat taacactaga aatctggtta gataataaat ttttaaatgt taagatacct
     5101 gttgaaaaaa tagaagaata cgggaacaac tatattactt ctatagagtt atttcctttc
     5161 tttggtgctg cttcagatta tgagaaagga tatgtgtttt accccgacgg aagtggtgga
     5221 ctatatcatt tcaaagggaa acctgaaatt gcagccgaaa agtatagctg gcctatttat
     5281 ggctatgata agttgaatct agatgagatc gaaaagagag agaaagcaga attaaagcaa
     5341 gcgcttcttc ctgtgtttgg aatgaaaaaa ggcgataacg cctttgtcgg tatagtcaca
     5401 aagggagagt acgatacaag tataaatttt taccctagtg gttatgctgt gaatctcagt
     5461 agagttaata tagagttttt ctacagaagg cttcataaag atttaagacc atacgcaact
     5521 gtaacagatt atatagaaaa aggaagaatc aatgacgatc atgaaataaa atataatttt
     5581 cttgtaaacg aaaacgcgga ttatagtggg atggcttccg cctacagaga gtaccttgaa
     5641 tcaatagacg caatacagta tacagtaagt ccaaatgaag atataccttt ttggttggat
     5701 ttgtttatgg ggataaaact cgatcaggtt ctattcgata agtatataaa gatgactact
     5761 tttgaagaag tagaaaaaat actggaaaac ttcaaaaaca aaggcgtgaa taatataatg
     5821 gcaaacctta ttggctgggt aaagggagga tatggaaatt ttcctgctaa ctggccgcca
     5881 gagaaagccc ttggaggtac aaaaggtatt ataaagcttg caaattatgc aagcaataat
     5941 aatatgaaac tgtttttgga aaacaattta gtggatgcgc tggccaaaaa tagaggattc
     6001 tctaaacgca atgatgtagt gtatcacaga aatggtcttt cagtgacaga tgaaaaaaga
     6061 gagaaataca ttttaaaccc tgtaaaagtg ttcgaaaggt tcatagaatc tcttatacca
     6121 aacataaaaa aagtacacat atcaggctta agttttgata gattgggaag ttttatatat
     6181 tatgattatc acaaaaacaa ccctgttaca agatgtgaga cagctgaact gtggactaga
     6241 attatggaag aaacgagaat agaaataggt agtactgcag ttagaggtgg tgacgcttac
     6301 actttgaaat atgcagattg gctgctagat atacctgttg aagactcagg ctattttatt
     6361 actgatgagg cagtgccatt ttatcagatg gtagtacatg gatttatacc ctattcttca
     6421 gaggcgggca accttttcca tgactttcaa agacagaagc taaagtggat agaatatgga
     6481 tgtatgcctt attttaaatt tacgtatcaa gaaccagtac taattagaaa aacggaatat
     6541 aaccatcttt ttacttcata ttatttgcat tggatagata tcgcagttga tatatatcaa
     6601 gaatttaatg aaaaaattgg agaattgtgg tcagtaaaca ttattaagca tgaaaaagtt
     6661 ttggagaatg tatataaggt tacttacaaa aatggtagca aaatctatat taattacaac
     6721 gaagattctg tgtatattga taactttagc ataaaaggaa tggattatat tgttgttgat
     6781 aaggggggaa caaaaaaatg aaaaaattta gattaaaaat gaagtataag cacatgctag
     6841 aagggtatat cttattgtta ccgtggatta ttggtactgt tttattcttc attgtgccaa
     6901 tataccaatc aattttttta agtttcagta agcttgttaa aataactgaa ttcaaaatga
     6961 aatgggtagg tctaatacat tatcaaagag cattattctt cgatatatgg tttgttcctg
     7021 tgtttataag catgattaaa gaaaccctaa ctaatacacc cttaataata atattttctc
     7081 tttttattgc tatgctttta aataaaaaca caaagttcaa aggatttttt agaggagtat
     7141 atgttttacc catagtattg ggtacggggt atgtaatgag tcagctttta ggtatacgag
     7201 taatttcaag ccctgaggga gctcctccac ctatggttgt ttctggtgag ctcagcagag
     7261 gtcttttaat tccagaagaa atgcttatat atttgggacc aacaataacg aaggctgtaa
     7321 cactatttct tagtaaaatt actatgatgc tctggaaatc aggagtacag attgtgcttt
     7381 tcctaggagg attgcaaagt atctcgaagt ccctgtatga ggcagcccat tgtgatggcg
     7441 ctacagaatg ggaaatgttt tggaaaatca cgtttcctat aatatctcca actatacttt
     7501 taagtattgt ttacactata gctgactctt ttacagatgc aagtaatcct atggtacaaa
     7561 aggtgatgca gttaggtttc aacaccgtgc agttcgagta tgcagcagct atttcctgga
     7621 tatatcttgc ctttataatg ctactaatag gattagtatt tctagttttt agaataggtc
     7681 aaaatagtat tgaaagcaaa aaagtggagg tataaaatgg taatgatggg aaaagtattt
     7741 aaaaaagttc tttctccaaa cgaaaaagat atgtattgga gcaaaaaagg aggtctgaaa
     7801 aatgagcaag ataatcctaa actaaggaaa cttaagcaat taagtttttg gagaaggaat
     7861 ttttataaga ctgctaatta tttaatactt gcagatcttg cttttgtgtt tttattgcct
     7921 ttcctgtaca tgattatcac ttctctaaaa acagatgcgg atctggtaga tactactgtt
     7981 aaatggattc ctactactat atgtatagaa aattatgtta gagctttcag agctcttgac
     8041 tatgtaaagc atttaaaaaa ctctgttttg gtaacagggc tttgtactgc aggtcatgta
     8101 ttttcatgtt ctcttgctgg atattcactt gcaaaatata gatattatgg aagaaattta
     8161 ttctttacat ttgtaatact atctattata actccgatac aagttatatt attgcctctt
     8221 tatttaaggt atggaaagct taatatgctt gggacatact taccactaat ccttcccacg
     8281 ttttttggat atggtttgag tggagcactt tttatattta tttacaggca gtatttttta
     8341 ggaattccac aggaatttat agaagcagca aagatagatg ggtgtggttt tataagcaca
     8401 ttttacagag ttgtttttcc tacatcaaaa tcacccacac ttgtaactac tgtactttcg
     8461 ctcatatggc attggaatga ctatttcgaa ccaacatttt atataagaag aagtcatttg
     8521 catctactat cacaaaagct accaaacata taccacctat tccaacaagc agggaaattt
     8581 gatccggtaa cacaacttca aataaaggta tataatgaag cagtatttat ggcagcgata
     8641 tttcttgtag tattgcctct acttgttata tatattgtgc tacagcgtca gtttgttgaa
     8701 ggaatagaaa gaacaggaca tgtagaatag gatatgttag gaaagttagt tgaagtgatt
     8761 gaataagttc aataaatatc atataacact atcgcgaagt tgtacacaaa tattatacga
     8821 aagaatatga aattatggag gtaagattat gaggaaaatc aatattacga ctacaaatcc
     8881 ctatactgtg aaacccgtag agcctgaaat acctgtattt acaaaagaag agtttgaaga
     8941 taggatctca agattgctta ataagatgag taatagtgag ttgacgcatg ttattgtata
     9001 tggagataga gagcattttt cgaatttaga ataccttaca ggttttgatc ctaggtatga
     9061 agaaggaatg cttattattc aggnnnnnnn nnncagggtg atggaaaatt aactttatta
     9121 gttggcaatg aagggtggaa ttactctttt atttcgccac ttaatttaga acgaattctg
     9181 tatcgaaact ttagtctgca gggacaacct agagataatc aaccgtgtct taaggaagta
     9241 tttaaaaact gcggaataga caaatattcc aatattggtg ttatcggaca aaagtatttc
     9301 gaatcagatc atataaaagg ttgtgaacat gaagctgggt attttactga tgtaccatca
     9361 tacataattg atattcttgt tcaaattgtc gacagaaaca gaataaccaa ttttacccat
     9421 gcaatgacaa atttatctga tggacttaga attacggtga aaggtgctaa agagatagct
     9481 tattacgaat atgtaacaaa caaagtatca aattgtgtaa ttaatatgtt agaaggacta
     9541 gaacctggta taagcgaact tgaagcatcc cggaaagctg gctacgatgc atctccaata
     9601 aatatgtttc cgcttgttaa ctttggagaa aagcatgcat ttttatgttt aaggagccct
     9661 aaccataata agttaaagga aggtaatcta gtaacgattt gctatggtat tcgtgggggc
     9721 cttgttgctc gctcgggtct tgcagtaaaa gacaagtctg gtatttctaa tagcttaggt
     9781 aatattatag aagaatttta tatgccatac tttagggctg ttatcacatg gtacgagtca
     9841 gtaaaaattg gcaaaaactg cggagaagta tacaataagg ttatggatat actcggaggg
     9901 gagaaagact ttggagtatc attaaatcca ggacataata ttagtagcga tgaatggacg
     9961 aattctcctt tttataaaga ttctccacat aaaatactta atgggtatta tctccagtgc
    10021 gatattattg cttctttgca aaatcctgta aggctagcaa tgcttgaaga tggagttata
    10081 tttgcagatg aaaaacttag agaagaattg aagtcacaat atccggatgt gtacaacaga
    10141 atccaaaaaa gaagagagtt tatgatagag gtcattggta ttaatttaag tgatgatgtc
    10201 cttcctttat caaatgctca agctatatgt catccatttt tattagatac aaataaaata
    10261 tttacaatag ataaattaat gaaataatcc ctaaaaccaa gcttaagggg gaataaagaa
    10321 tgagttttaa aaatgaaagt tgttttctag aactgaactt atgtgatatt aattctcaat
    10381 taaaaagtta tatttataaa ctttctgatg aggcttttct tctaggagat aatgctaggg
    10441 actcaattaa tagtattgga gaatttgaaa aacgcagaac ttatttacgt gagaagttca
    10501 ttgaatcaat gggagggcta agttcattag aaactccgtt aaaccctgta gttacaggaa
    10561 ccatagaagg tgatgggttt agaatagaaa aagtaatttt agagtcaagg cctaaaactt
    10621 atgtaacttg taacctatac atacctaaca atattgataa gccagtaggt gctgtcttat
    10681 tattgtgtgg tcactcacaa aatggaaagg aagattatca aattgcatgc caatattttg
    10741 caaaagcagg gatggtagtt ttagtacaag atccaattgg gcaaagcgaa agattaagct
    10801 attatgatgc tgctactgga aaaattaata taacatgggg gacgtatgaa cataattatg
    10861 caggaagcca gagtttgttt ttaggtgaag gaattgcacg ttattttctg catgattccg
    10921 ttagagcaat agattatctt tgtacacggc ctgaagtaga tcctactaaa ataggagtta
    10981 caggatgctc tggaggaggg acgcaaacat gcatgttgat gctttatgat caacgtatag
    11041 cagcagcagc tcctg
//
LOCUS       DAOSDD010000146        11016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2280_length_11016_cov_5.530791, whole genome shotgun sequence.
ACCESSION   DAOSDD010000146 DAOSDD010000000
VERSION     DAOSDD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2280_length_11016_cov_5.530791"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK733_12670"
     CDS             <1..838
                     /locus_tag="PK733_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOJ11427.1"
                     /translation="KEWNFNIVKTGDTVKTGKYELVFVEMTMLHWPDSLLTYVKGANL
                     VFSNDAFGQHFSGPAFFNDEVDRCELYQEAMKYYANILLPYSPLVKKKVEQIKAMNLD
                     IDMIAPSHGIIWRENPMQIVEKYYEWCQDYKEDFVSIVYDTMYDSTKAMAEAIGQGLM
                     EEGIKFKIFNASVTDRSDLLTELFKSRGIIMGSCTVNNTVLRPITSLLEEIKALKFKN
                     KAAAGFGSFGWSGEAAGIISRKLEACGLKVSLQPLQFKYRPTEAELEQCKEYGKEFAK
                     YI"
     gene            883..1833
                     /locus_tag="PK733_12675"
     CDS             883..1833
                     /locus_tag="PK733_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ11428.1"
                     /translation="MNADQIKELGAKIRENISKVIVGKENVIDLAITALFCSGHVLLE
                     DVPGMGKTVLAKCLAKSINCDFKRIQFTPDLLPSDLTGINYYNQKQGEFVFREGPVFT
                     NIILADEINRATPRTQSSLLECMEERQVTVDGETKLLSRPFFVIATQNPVEIQGTFPL
                     PEAQLDRFFMRLKIGYPTAEEGRSILDRFQKANPLPSITNVTNAAEIVEAQDSIALIE
                     VSDDLKNYIIQIVEMTRAHEKIILGISPRGSIALMRSAQAYAVINGRDFVTPDDVKHV
                     APAVLSHRIVLKSHAITSGADNAENVITDILNKVPVPLED"
     gene            1838..2977
                     /locus_tag="PK733_12680"
     CDS             1838..2977
                     /locus_tag="PK733_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ11429.1"
                     /translation="MEVLALAVIAFAIIGFQAFIFRRFAFKNLKYKCEFSINEAHEGD
                     DIYLIETVYNGKLLPVPWLKAELNSSKWLDFAETRSVVAQENRYVTSSFFLKSHQKVI
                     RRWKLKCIKRGVFHIDRVSLVSGDLLGNAIDSMGIDVNTYLKVYPRTIELEKTFIPVN
                     HLQGDAIVRRWIMEDPFIVAGTREYTSSDPMNKIHWASTAHAGRLMVKREDYTSQNGT
                     AVFLNIQSIENEYFDAVYKDYIELGIKAAATIFDTALRNGIPVRFATNGTTVDDGRQM
                     IFTSQASGRSHISSLLGILAMLQLRRLKDFEDFLRDVHNDIVNSEVILITSYLTVPIC
                     DLLRRMKMKNNRVKIMVTNKTIEPHTLPHDMDVYILKEAVILDGE"
     gene            2967..4427
                     /locus_tag="PK733_12685"
     CDS             2967..4427
                     /locus_tag="PK733_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11430.1"
                     /translation="MGSKLWKVFKREVSLLAIFAYLYQLILLPRTVFFKDTGGVPGVM
                     AGICLLFGGYIIAGLLTKKKQSFLRMLISYLSSIIPALLFTIFYWKGFPFIEKGIVYS
                     LWAQNEYVYVLWRGILEGIGAFLLYFAGVRLKFLDYDSILSRNKLIAAGIIFVSAIIF
                     VEYYNYKELEYLKSTLYAFVYIFIVIVLLVKNQQNLDRAFIKKHIDLATVPGKIRNYN
                     SLIVISIFLLIMFIFNIKALVSFIVRVIKNTPRVIITIMFKILEILSMLFPGSSEGGQ
                     SEGGTPQLPMLPEEEKNAILSLIMEIILWLILAAMAVFIIYKLPKVFAFIGRKLKKAG
                     VYILDTIRKLFLINKDYPDHETDYVDEVLNVKPEAPDKDNKNKGNKIHRMGGRLWRNA
                     EPVEKIRFMNGVILSYMKKSGMDIQKSNTAQEVCGKAVEIYDGIPENDGLKESLGITT
                     RVYEKARYGGKAPDIEEYTCFEEKFTQAVDIIKRNS"
     gene            4671..7124
                     /gene="lon"
                     /locus_tag="PK733_12690"
     CDS             4671..7124
                     /gene="lon"
                     /locus_tag="PK733_12690"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ11431.1"
                     /translation="MSEIEKTIKHELPLLPLRGLTVFPYIIMHFDVGRVKSIKALEQA
                     MINNQLIFLAAQKDAKDDSPGPEGVYNVGTISRVKQLVKLPGDNIRVLIEGISRAEIS
                     EFTQKEPFFMVEVLEKSDMMEDEPENIIEALKRKVMGTFEEYTKLSNKISQDMFLTIS
                     EIENAGQICDMIISSMFLKTEQKQEILNEYRIVPRMEKLLEILIKENEILEIEMNISS
                     KVRKQIDKMQKDYYLREQLKVIQNELGDRDGIAGEVDEYKQKLENANLPEEVEFKVNK
                     ELDRMVKLPPGSAEGGVIRTYLDWIFDLPWNTRTEEIIDLKKAQEILDEDHYGLEKVK
                     ERIIEYLAVRKLTNSLKGPIICLVGPPGVGKTSIAKSISRALNRNYVRMSLGGVRDEA
                     EIRGHRRTYVGAMPGRIISAMKQAGSMNPLILLDEIDKMSSDFRGDPASAMLEVLDGE
                     QNFAFRDHYLEVPFNLSDVLFLTTANNMETIPRPLLDRMEVIAISGYTEEEKVNIAAK
                     YLVPKQISAHGLEKKNLKIEESAVRDIISCYTREAGVRNLEREIAAICRKAATQIAGE
                     NRKTIRVTSGNLEKHLGTKKYRYDKANDKDEIGIATGLAWTPVGGDTLSIEVTVMDGS
                     GKLELTGQLGDVMKESARAAMSFIRSRAQEFGIEKDFYTKYDIHIHVPEGAIPKDGPS
                     AGITLATALVSALSNIPVRKNVAMTGEITLRGRVLPIGGLKEKVLAAHRAGIDTIIIP
                     VDNKKDLDDIPENVKKTIKFVYASNMDIVFSTALVRHKKKAKHIAKNLFDEEDNNMVL
                     HDQQPIIGNIEDNASIMKQ"
     gene            complement(7328..7951)
                     /locus_tag="PK733_12695"
     CDS             complement(7328..7951)
                     /locus_tag="PK733_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HOJ11432.1"
                     /translation="MKIERISDNMIKVTISSNDLEERNIDLQSLTYNSVAAQELLWDM
                     MEQAELQYGFDFSNSHIVFEPLSDALDGFIITITRLDEDADFESLQKYIKNRLKKNDP
                     RIRKKTRRILYPARIIYLFNSLDDICDIAEKIQSIFSGESYIYKLKNSYYLILKSIKP
                     FNYRKLEVLLNEYGNKVLSTAFYEGYLNEYGTLMIEKNAINVLASYF"
     gene            8436..8708
                     /locus_tag="PK733_12700"
     CDS             8436..8708
                     /locus_tag="PK733_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11433.1"
                     /translation="MRRSENPLRFFVFIIVGAVLGGILGEILSQIQGLAWIKMGGVNG
                     YRELLSFSFDPLINTNFLRFGLNLTIRVNFGNLVGIITGVILYLKT"
     gene            8738..9346
                     /locus_tag="PK733_12705"
     CDS             8738..9346
                     /locus_tag="PK733_12705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOJ11434.1"
                     /translation="MSKIREENANRIILASASPRRSDLLKQAGIPFLVVPGEVNEESL
                     NLEGEPEKQAEKSALIKALDVAEKFGTGLVLGADTIVVFDNQIYGKPGDKKHAYNMLK
                     SLQGRIHHVITGIAIVDASTGVYKTAHEKTSVYFTSLNDDEIWSYINSGEPFGKAGAY
                     AIQGKGALFVEKIDGCYTNVVGLPLLRVKKLLEEFCLKYRNL"
     gene            9695..10393
                     /gene="radC"
                     /locus_tag="PK733_12710"
     CDS             9695..10393
                     /gene="radC"
                     /locus_tag="PK733_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ11435.1"
                     /translation="MSIKLKIKDLPVNERPYEKLEKYGPEMLSDAELLAIIIKSGNKN
                     ETSVSLAQRVLAQDEGGKGLISLHDFSLEQLRLVNGIGRVKAIQIKAVMELSKRVAST
                     FSNNGKIVIKSSYDVSKLLMEEMRHLKKEVFKTLLLNTKNHLIKQINVSVGSLNASIV
                     HPREVFSEAVKSGCSAVIFIHNHPSGDPEPSMEDIETTRKLLSAGDVLGIKVLDHIII
                     GDGVYISLKEKGFI"
     gene            10418..>11016
                     /locus_tag="PK733_12715"
     CDS             10418..>11016
                     /locus_tag="PK733_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ11436.1"
                     /translation="MSLFGKDIGIDLGTANTIVHVKGKGIVIREPSVVAINKKTNTVI
                     TVGDAAKEMIGRTPGDIVAIRPMRDGVIADYDVTLNMLKYFIKKTVPSTLFSKPRVII
                     CVPSGVTEVEKRAVEEATYQAGGREVGLIEEPMAAAIGANLPVEEPSGSMVVDVGGGT
                     SEVAVISLGGIVTSRSLRVAGDDFDESIVNYIKKEYNLM"
BASE COUNT         3810 a         1617 c         2337 g         3252 t
ORIGIN      
        1 taaggaatgg aactttaata tagttaagac aggtgatact gttaaaactg ggaaatatga
       61 acttgttttt gtagaaatga caatgctcca ttggccggac agtcttctta cttatgtgaa
      121 aggtgcaaac cttgtttttt caaacgatgc atttggccag cacttttcag gacctgcatt
      181 ttttaatgat gaagtagaca gatgtgaact ataccaggaa gctatgaagt actatgcaaa
      241 tatattgctg ccatatagtc ctcttgttaa gaaaaaggtt gagcagataa aggcaatgaa
      301 ccttgacatc gacatgattg cgccgagtca tggcattatt tggcgtgaaa accccatgca
      361 gatcgtggaa aagtattatg aatggtgcca ggattataag gaggattttg tatctattgt
      421 atatgatacg atgtatgatt ccacaaaagc tatggcggaa gctattggcc agggtttgat
      481 ggaagaaggc atcaagttta agatattcaa tgcttcagtt acagacagaa gcgacttgct
      541 tactgagctc ttcaaatcaa ggggaattat tatgggcagc tgcacagtta acaatactgt
      601 attaaggcca ataacatctc ttttagagga gattaaggct cttaagttta aaaacaaagc
      661 agcagcaggt tttggaagct ttggctggag tggagaggcg gcaggcatta taagcaggaa
      721 acttgaagct tgcggcttga aggtatcgtt acaaccgctt cagtttaaat acaggcccac
      781 tgaggctgaa ttggaacagt gcaaggaata tggaaaagaa tttgcgaaat atatttagat
      841 gcttagtaga atcatataaa aaataattag gggttaagtg ttatgaatgc agaccagatt
      901 aaagaattag gtgccaaaat cagagagaat atttccaagg ttattgttgg caaggaaaat
      961 gttattgacc tggcaataac tgcccttttt tgttccggac atgttttgct ggaagatgtg
     1021 cccggcatgg gaaaaactgt tctggctaaa tgccttgcca aatcaataaa ctgtgatttc
     1081 aaaagaatac agtttacacc ggacctgctt ccttcagact taacaggtat aaattattat
     1141 aatcagaaac agggagaatt tgttttcaga gaaggacctg tatttactaa tattatttta
     1201 gcagatgaga taaaccgggc tacgcccaga acacaatcga gtcttcttga atgtatggaa
     1261 gaacgacagg taacagtgga tggtgaaaca aaactccttt caagaccgtt ttttgttatt
     1321 gcaacccaga acccggttga gatacaggga acatttcctt tacctgaagc tcaattggat
     1381 cggtttttca tgaggctgaa gataggctat cctacagcag aagaaggcag atccatactg
     1441 gacaggtttc aaaaagcaaa tccgcttcca tcgataacta atgtaacaaa tgcagcggag
     1501 atagttgaag cacaggattc aatagcttta attgaggtaa gcgatgattt gaaaaattat
     1561 attattcaaa ttgtggaaat gacaagggcg catgaaaaaa ttatattggg tataagtccc
     1621 agaggcagca ttgccctgat gaggtctgca caggcttatg cggtcattaa tggaagagat
     1681 tttgttacac cggatgatgt aaagcatgtt gcaccggcgg ttctttcaca caggatagtc
     1741 cttaaaagcc atgcaattac atcgggagcc gataatgcag agaatgtaat aaccgatata
     1801 ttaaacaagg ttcctgtgcc tttggaggat tagatatatg gaagtacttg ccctggctgt
     1861 aattgcattt gcaattatag ggtttcaggc ctttatattc agaaggtttg cttttaaaaa
     1921 tctgaagtat aaatgtgaat tcagcataaa tgaagctcat gaaggagatg acatttatct
     1981 gattgaaact gtttataatg gcaaactgct tccggtacca tggctgaaag cagaactaaa
     2041 ctcatcaaaa tggctggatt ttgcagaaac cagatctgtt gtcgctcagg agaacagata
     2101 cgtaacaagc agcttctttc tgaaaagtca tcaaaaagta ataagacgat ggaaattgaa
     2161 atgtataaag cgtggggtat ttcatattga cagggtatca cttgtatccg gagatttgct
     2221 tggcaatgcc atagattcaa tgggtattga tgtaaataca taccttaagg tatatccccg
     2281 gactatagaa ctggaaaaaa cctttatacc tgttaatcat ctccagggag atgcaatcgt
     2341 tagaaggtgg attatggaag atccatttat agtagcaggt acacgggaat atacttcaag
     2401 tgatcctatg aataagatac attgggcttc aactgcccat gccggacgct taatggtaaa
     2461 acgggaggat tatacctcgc aaaatggaac tgctgttttt ttaaacatac aatctataga
     2521 gaatgaatat tttgatgccg tctacaaaga ttatattgaa ctaggcataa aagctgccgc
     2581 gacaatattt gacacagcac tgagaaatgg aattccggta agatttgcca ctaatggcac
     2641 tactgtagat gacggacgcc agatgatttt tacaagtcag gcttccggca ggtcacatat
     2701 ttctagtttg ctaggtattc ttgcaatgct ccaattaagg agattaaaag actttgaaga
     2761 tttcctgcgg gatgtacata atgatattgt taattcagaa gttattttaa ttacgtcata
     2821 cttgacagta cctatttgtg atctgttaag acggatgaaa atgaaaaata acagagtgaa
     2881 aataatggtt acaaataaga ctattgaacc gcatacttta ccacatgaca tggatgtata
     2941 cattcttaag gaggcagtga tactggatgg ggagtaagct ttggaaagta ttcaaaaggg
     3001 aagtatccct tttggcaata tttgcatact tatatcagtt gatacttttg ccaaggacag
     3061 tattttttaa ggacacaggc ggtgtgcctg gagtgatggc aggaatctgc cttttatttg
     3121 gaggctatat tattgccggg ctcctcacaa aaaagaaaca aagctttcta aggatgttga
     3181 tatcatattt atcatcaata attcccgcat tattatttac catattttac tggaagggat
     3241 ttccctttat agagaaagga attgtttatt ctctctgggc gcaaaatgaa tatgtatatg
     3301 ttttgtggcg aggtatttta gaaggtattg gtgcatttct actatacttt gcaggtgtaa
     3361 ggttgaaatt ccttgattat gactcaattt taagcagaaa caagttaatt gctgctggaa
     3421 ttatatttgt ttctgcaatt atatttgttg aatattataa ctataaggaa cttgagtatt
     3481 taaaaagtac cttgtatgct tttgtatata tttttatagt aattgtcctg ctggtaaaaa
     3541 atcagcaaaa cctggacaga gcatttatta aaaaacatat tgacctggca accgtgccgg
     3601 ggaaaataag aaattataac tcattaatag taataagcat atttttatta attatgttta
     3661 tatttaacat aaaagcactt gtttccttta tagtaagggt tataaaaaat acacccagag
     3721 ttattataac tataatgttc aaaattcttg aaattctttc aatgcttttt cccggaagta
     3781 gtgaaggcgg tcaatctgaa ggtggaactc cgcaattgcc catgcttcct gaggaagaaa
     3841 aaaatgccat attaagcttg atcatggaaa ttattctttg gttgatttta gcagctatgg
     3901 ctgtttttat aatttataag ttacccaaag tattcgcttt tatagggagg aagcttaaga
     3961 aagcaggggt gtatatttta gatactataa gaaagctttt ccttataaac aaagattacc
     4021 ctgaccatga aactgattat gttgatgagg tattgaatgt aaagccggaa gctcctgaca
     4081 aggataataa aaacaaaggg aataaaatac accgtatggg tggaaggctg tggcgaaatg
     4141 ctgaacctgt agaaaaaata aggtttatga atggagtaat cttaagctat atgaaaaaaa
     4201 gtggtatgga tatacaaaaa tccaatactg cccaggaagt atgtggcaaa gcagtagaaa
     4261 tatatgatgg gataccggaa aatgatgggt tgaaggaaag cttgggaatt acaaccaggg
     4321 tttatgaaaa ggccagatat ggggggaaag cccctgatat tgaagaatat acttgttttg
     4381 aagagaaatt tacacaagct gtggatataa taaaaagaaa ttcataagaa tatttcatgt
     4441 aatacttacc tttagtacca tataaaacaa ttgaaattaa gattcattaa ggttataata
     4501 atgtcgaaat acgctgcatt atgtaaacat gtcggtataa gttatttatt gaccgtggaa
     4561 tgcataaatt gaactatggt gcaaaaccca ttaaatcaaa acattatgaa atttgaaaaa
     4621 tctacgtata tgcagtttga ttataaatat taatttaggg gttggtcaaa atgtctgaaa
     4681 tagaaaaaac aataaaacac gaattaccat tattaccttt aagaggtctt actgtgtttc
     4741 catatataat aatgcatttt gatgtgggga gagtaaaatc aattaaagcg ctggaacagg
     4801 ccatgattaa caatcagctt atttttcttg ctgctcagaa agatgctaaa gatgattcgc
     4861 cagggcctga aggggtttat aatgtaggaa caatatcaag agtaaagcag ctggtaaaac
     4921 ttccgggaga caatataaga gtcctgattg agggtataag cagagccgaa ataagtgagt
     4981 ttacccagaa agaaccgttt tttatggtag aagttcttga aaaatcggat atgatggaag
     5041 atgaacctga gaatataatc gaagctttaa aaagaaaagt tatgggaaca tttgaggaat
     5101 ataccaaatt gagcaataaa atatctcagg atatgttcct tacaatttcg gaaattgaaa
     5161 atgcaggaca aatctgcgat atgattatat ccagtatgtt tttaaagacc gagcagaaac
     5221 aggaaatttt aaatgaatat agaattgtcc ccaggatgga aaaacttctt gagattctaa
     5281 ttaaagaaaa cgaaattctt gagatagaaa tgaatataag cagcaaagta cgcaagcaaa
     5341 tagataaaat gcaaaaagat tattatttaa gagaacaatt aaaagtcata cagaatgaat
     5401 tgggggacag ggacggaata gcaggagaag ttgatgaata caagcaaaaa ctagaaaatg
     5461 caaatctacc tgaagaggtt gagtttaagg ttaacaagga acttgaccgg atggtaaaat
     5521 taccgccagg ttctgcagaa ggtggagtaa tacgaactta tctggattgg atatttgatc
     5581 ttccatggaa taccagaaca gaggaaatta ttgacttgaa aaaagcacag gaaatattgg
     5641 atgaagacca ttacgggctc gaaaaggtaa aagaaagaat tatagagtat cttgctgtaa
     5701 ggaagcttac aaacagcctt aaaggtccca taatatgcct tgtaggtcct cccggagtcg
     5761 ggaaaacttc tattgcaaag tctatttcca gggcattaaa caggaactat gtgagaatgt
     5821 cacttggggg agtgagggat gaggctgaga taagaggcca tagaaggact tatgtaggtg
     5881 caatgcccgg cagaattatt tctgctatga aacaagcagg atctatgaat ccattgattc
     5941 tacttgacga aatcgataag atgagcagcg atttcagagg tgatcccgca tcagccatgc
     6001 ttgaagtact tgatggtgag cagaattttg catttagaga tcattacctt gaagttccat
     6061 ttaacttgtc agatgtgctt ttccttacta cggcaaataa tatggaaact atccccagac
     6121 ctcttcttga cagaatggaa gttatagcaa tatcaggata tacagaagag gaaaaggtaa
     6181 acattgccgc caaatatctt gtaccaaagc agattagtgc tcacggactt gaaaaaaaga
     6241 accttaaaat tgaggaaagt gcagtaagag atattatttc atgctacacc agagaagcag
     6301 gagtaagaaa tcttgaaagg gagattgcag ctatttgccg taaggcggcc acgcaaatag
     6361 ccggagagaa caggaagact ataagggtta cttcgggaaa tctggagaaa caccttggta
     6421 ccaagaaata caggtatgat aaggcaaatg ataaggatga gattggtatt gctacaggac
     6481 tggcctggac tcccgtaggc ggggacacat tgtccataga ggttactgta atggatggga
     6541 gcgggaagct tgaattaaca gggcaattgg gtgatgtcat gaaggaatcc gcaagggctg
     6601 ccatgagttt tatacgttcc agagctcagg agtttggtat tgaaaaggat ttttatacaa
     6661 aatatgatat ccatatacat gttcctgaag gtgctatacc taaggatgga ccttctgcag
     6721 gaataacact ggctactgct ttagtttcag ctttatcaaa tatacctgtc aggaaaaatg
     6781 tagcaatgac aggtgaaatt acattaagag gcagggtact tcctataggg ggattgaagg
     6841 aaaaagtcct tgcagctcat agggctggaa tagatactat tattatacct gttgataata
     6901 aaaaggatct tgatgatatc cctgaaaatg taaagaaaac cataaaattt gtatatgctt
     6961 caaatatgga tatagttttt agtactgctt tggtaaggca caaaaagaaa gcaaagcata
     7021 ttgcaaaaaa tctttttgat gaagaggaca acaatatggt cctccatgac cagcagccaa
     7081 taatagggaa tatagaagat aatgcttcta taatgaagca atagtatata agttggtagt
     7141 ggatagtggg tagagggtag tgggtagtgg atagtgggta gagggtagtg ggtagagggt
     7201 agtggatagt tagaaattgt tcagtggggg ttgtaatccc ccactaaatc ccatctaaca
     7261 tctcacctct aatgtcttac cttcaacctc ttacctctca cctctaacag gtgtgtatgt
     7321 cctcacctta aaaataggaa gcaagcacat ttattgcatt cttttcaatc attaatgtac
     7381 catattcatt tagatagcct tcataaaatg cagtgctcaa aactttattt ccatattcat
     7441 tcaataatac ttccaatttt ctataattaa atggctttat actctttagt attaaatagt
     7501 aggaattttt tagtttataa atgtaacttt ctccggagaa tatagattgt attttttctg
     7561 ctatatcaca tatgtcatcc agtgaattaa acaaataaat aattcttgca ggatagagaa
     7621 tcctgcgggt tttttttctt attctggggt catttttctt cagcctgttt tttatatatt
     7681 tttgtagtga ttcaaaatct gcatcttcat ccaacctggt aatagtaatt ataaatccat
     7741 ccaatgcatc actcaaaggc tcaaaaacaa tatgggagtt tgaaaaatca aatccatatt
     7801 gaagttcagc ctgttccatc atatcccaga gcaattcctg tgctgccact gaattataag
     7861 taagggactg caggtctata tttctttctt ccaggtcatt tgaagaaatt gtaaccttaa
     7921 tcatattatc gcttattctc tcaatcttca tttaaagctc cattcctgca gcgtgcgaaa
     7981 tgaattcgcc cttgtaggag caaaactttt ttgtttaaaa aagtttttat gggcttaaat
     8041 tcattttagc cgcgcatgca atttattttt agaaatacct gtgcgaaaaa tatgcacatg
     8101 cgcattagtt tttcagaaat ttatcaattt ctttcaaact aattaccaca atcctttatt
     8161 tatattctga attttgaata aatagcaaaa ataaacccta ttaagaacag attacatttt
     8221 tgcatattta tcatggtagc ataagcgcat aaaactttat aaaaaaatta tatttatgaa
     8281 ttattatgcg ttacacccta taactatatt atacttctta aaaccgaaaa tgagaagcct
     8341 gaattttatc cgagcaaagt atttgaaata ataatataat tgagtataat aaatgtaatg
     8401 attgtcccga aaaataatga atttaggagg atattatgcg gcgttcagaa aatcctttac
     8461 gtttttttgt ttttatcatt gtaggtgctg ttttaggagg tattctgggt gaaatattat
     8521 cacagatcca gggccttgca tggattaaaa tgggtggggt gaatggttat agagaactat
     8581 tgtcattttc ttttgatcct ttgattaaca caaacttcct gagatttgga ttgaatttaa
     8641 ctattagggt taattttgga aaccttgtgg gaataattac aggagtaata ttgtatctga
     8701 aaacttaata gataagagaa caaagtgaag gagacatatg agtaaaattc gagaggaaaa
     8761 tgcaaacaga ataattttgg catcagcttc accgagacgc agtgatttgt taaaacaggc
     8821 gggaatacct tttttagttg ttcctggtga agttaatgag gaaagtctta atcttgaagg
     8881 agaacctgaa aaacaggctg aaaagtctgc tttgataaag gcactggatg ttgctgaaaa
     8941 gtttgggaca ggtctggtat tgggagcaga cactattgta gtttttgata accaaatata
     9001 tggcaagccc ggtgataaaa aacatgccta taatatgtta aaaagccttc aaggaagaat
     9061 tcaccatgtg attaccggta tagccatagt tgatgcatca actggagttt ataaaacagc
     9121 acatgaaaaa accagtgtat attttacttc tcttaatgat gatgaaatat ggagctatat
     9181 aaattctgga gagccttttg gcaaagccgg ggcatatgcc attcagggta aaggcgcttt
     9241 atttgttgaa aaaattgatg gctgttatac aaatgtagta ggtttgccat tactacgggt
     9301 aaaaaaacta ctggaggaat tttgtttaaa gtataggaat ttataaaatt cttaagaaat
     9361 tcctatactg gggtttggaa agaatgtgaa tgagacatcc tcctatgatc atgaataata
     9421 cataatacta cataaatggg aattataaat tatttatgta aaaaagagga aagccacctt
     9481 ccggtgtaga atatataatt aaagagttta gcaaaagacc ggggcagaat caaatattaa
     9541 cttgataaat agctttaagt aagtttataa tacatataag gttagaaaaa ttttctactg
     9601 atgggttata agacttttgt tttacccttg ggtgttgttg aactctaaac aattaaaaac
     9661 aacaataatg aaaaaaacac tgggggtaaa ttaaatgtca ataaaactta aaattaaaga
     9721 tcttcctgta aatgagagac cttatgaaaa acttgaaaag tatggacctg aaatgttatc
     9781 agatgctgag cttctggcaa taattataaa gtcgggcaac aaaaatgaaa catccgtttc
     9841 acttgcacag agggtacttg ctcaggatga aggaggaaaa ggacttattt ctttacatga
     9901 tttctctctt gaacagttga gacttgttaa tggcataggc agggtgaaag ctatacaaat
     9961 aaaagctgtt atggagcttt caaagagggt agcatcaact ttcagcaata acgggaagat
    10021 tgtgataaaa tcatcatatg atgtaagcaa acttcttatg gaagaaatgc ggcatttaaa
    10081 aaaagaggta tttaaaacac ttttgttaaa tacaaaaaat catctgataa aacagataaa
    10141 tgtctctgtt ggaagcttga atgcatctat agtacatccg agagaggtct tttcagaagc
    10201 cgtaaagtca ggatgttcgg cagtaatttt tattcacaat catcctagcg gcgatcctga
    10261 accgagcatg gaggatattg aaacaacaag aaagctttta agtgcaggtg atgttcttgg
    10321 aataaaagta cttgaccata taataattgg tgatggagtt tatataagtt taaaagaaaa
    10381 aggttttatt tagatataat aaacggagga taaaggtatg agtttatttg ggaaagatat
    10441 tggcattgac ttgggtacgg ccaatacaat agttcatgta aaaggaaaag gtatagtaat
    10501 aagggaacct tcggttgttg ctataaacaa aaaaactaat acagttataa ctgtaggtga
    10561 tgcagctaaa gaaatgattg gaagaacccc gggagacatt gtagccataa ggccaatgag
    10621 agatggagtt attgcggatt atgacgttac tcttaatatg ttgaagtatt ttataaaaaa
    10681 gaccgttcca tcaaccctgt tttcaaaacc gagagtaata atatgtgtac cttctggtgt
    10741 tacagaagtt gaaaaaaggg cagttgagga ggcaacttat caagccgggg gaagagaagt
    10801 gggacttatt gaagaaccta tggcagcagc aataggtgcc aacttgcctg tagaagaacc
    10861 ttcaggcagt atggtggtag atgttggagg aggaaccagt gaagtagctg taatatcgct
    10921 gggcggaata gttacaagca gatccttgag agttgcagga gatgattttg atgaaagtat
    10981 tgttaattat ataaaaaaag aatataattt aatgat
//
LOCUS       DAOSDD010000147        10995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2286_length_10995_cov_5.040768, whole genome shotgun sequence.
ACCESSION   DAOSDD010000147 DAOSDD010000000
VERSION     DAOSDD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_10995_cov_5.040768"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="hslO"
                     /locus_tag="PK733_12720"
     CDS             complement(<1..871)
                     /gene="hslO"
                     /locus_tag="PK733_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOJ11437.1"
                     /translation="MEDYIIRSVAAGNQVRAFAAVTTDTVSRAANIHKLAPVASAALG
                     RTLTAAAMMSKMLKSQDNIITIQIKGDGPLGGVVVVSDYQANVRGYVYNPEVYIPLNS
                     AGKLDVAGAIGEGYLNVIKDLGLKEPYIGYVDLVSGEIGEDIAYYFAYSEQVPSIVSL
                     GVLVNTDEKVLHSGGFIIQLLPGAETKTIDAIDDRIKSIPSVTSMLTDGKTPEDILEF
                     IFEGMELKITDKSPCKYKCNCSRDRMERNIISLGEKEIQDMIIEQNGAETVCHFCNTK
                     YWFDSETLKQLAEQ"
     gene            complement(976..1176)
                     /locus_tag="PK733_12725"
     CDS             complement(976..1176)
                     /locus_tag="PK733_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOJ11438.1"
                     /translation="MEKGKVKWFSAEKGFGFIEREGGNDVFVHFSAINMDGFKTLEEG
                     TEVEFEVVEGVKGPQAANVHRA"
     gene            complement(1348..2061)
                     /locus_tag="PK733_12730"
     CDS             complement(1348..2061)
                     /locus_tag="PK733_12730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ11439.1"
                     /translation="MSDIDYCKWADYIEKIFHYNNHKPKLIVDLGCGTGSFCIEMAKR
                     GYDTIGIDISSEMLSLAVSKSEDTEIAGDKLLFLNQDMSEFELYGTADAFVSLMDSVN
                     YVTGKRELKSMFKLVRNYLNPGGLFIFDVNSVYKFEKVLNNNVFYYINDDITYIWANN
                     YNKRTKICEFDLTFFVKDKSDLYKRFDEIHYERAYSVEEISRLINDNGLELIVVYDNL
                     SFNKPLKKSERLFFVCRKP"
     gene            complement(2229..2414)
                     /locus_tag="PK733_12735"
     CDS             complement(2229..2414)
                     /locus_tag="PK733_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOJ11440.1"
                     /translation="MEKENKRLKYEIAKELGLLDKVLKSGWKSLTAKETGRIGGLVTK
                     RMKKTGADNIGSKEGSI"
     gene            complement(2475..3713)
                     /locus_tag="PK733_12740"
     CDS             complement(2475..3713)
                     /locus_tag="PK733_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ11441.1"
                     /translation="MYKKSSVIIGIIVIVLVTSIITFSIAAVVFLGPVFNNKYQISFD
                     TGSVNYESIKKFNQARKALKDNYYEDVDENVLLEGAVAGMAASLDDPYTVYYTKEQMD
                     KILEIPKKSEETYVGIGVSIIADKDGIVTVVEPFTGTPAYEAGIKQGDKIIKVNDEDV
                     TALKDESTVVKLIKGPENTTVKITVYRPSEGRPIDFEVSRKKITVQLNIRSEVLQDNI
                     GYIRLISFMDDKINKEFEEHLKKLLDQNIKALVIDVRDNPGGYYNKVVNIVDRIIPEG
                     LIVYTEDRQKNVQEERSDKRELEIPLAVLINENSASASEILAGAIKDHKKGALVGTKT
                     FGKGLVQNLLPLDDGSGIKITISRYFTPSGVCIHGTGIEPDINIDLDEKYKYTPISQI
                     PREDDIQLKKAVEILNRLAS"
     gene            complement(3961..5097)
                     /locus_tag="PK733_12745"
     CDS             complement(3961..5097)
                     /locus_tag="PK733_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ11442.1"
                     /translation="MNKKRAISVLLISIMIITWTFQVLADADELDKAKQDKKNIDSQI
                     NQINQQKKNELSAKNKLEQEKKDVIGTEKKESQEYDELIKEIAELEKTLKEVEKALEE
                     AEQNYEKQCNSFKARLRAIYKNSNETILDMLFESKSITAFLEKLEFISLLSKKDKNDM
                     DELELAKKEVECKRQLIAEQKVELQKVIEEKKQRLDSLKASRTNLDQKIQSSKSRLAT
                     LEKQEKELLAKSEEMGNLIITLSSKSKYVEGNMVWPLPSSSLITSEYGNRKHPILKTY
                     SMHTGVDIGGKSGANILAANKGTVIISGWQNAYGYTVVVDHGGGITTLYAHCSKILVK
                     KGQEVKAGQIIAKVGSTGWSTGPHLHFEVRVNGATKNPLNYVKP"
     gene            complement(5221..6120)
                     /gene="ftsX"
                     /locus_tag="PK733_12750"
     CDS             complement(5221..6120)
                     /gene="ftsX"
                     /locus_tag="PK733_12750"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOJ11443.1"
                     /translation="MKIRTIKHIFKEGITNVYRNKLMSLASISIVVASLTIFGIFYMI
                     ILNFNHNVGKLKDQPEMQVFCLTELDDVGVKIVEDALMQNEKIKQVAIVTKEQAFEKV
                     KDMLGDNASVLEGIDNSFLPVSFIIKLNNPEDSAMLMDELEKINGVDKVTYPQKTVEF
                     ISKFTHGIQIVNSILTTVLLVVSVFIISNTIKLTVFARRREISIMKYIGATDWFIRWP
                     FIVEGVIIGFIGSIVALLISGYGYNAVEMRFTKELLMTGTSIISILKLNDVSARVALL
                     YIVLGCIVGASGSVTTIRKYLKV"
     gene            complement(6089..6796)
                     /gene="ftsE"
                     /locus_tag="PK733_12755"
     CDS             complement(6089..6796)
                     /gene="ftsE"
                     /locus_tag="PK733_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOJ11444.1"
                     /translation="MIEFINVSKKYSNGTLALSNVNLKINKGDFVFIVGQSGSGKTTL
                     IKLILKEEDLTEGEIFVNGYEVSSMSRSEIPYLRRSLGVVFQDFRLLPDKTVYDNVAF
                     AMQITESLPKEIRRQVPMALGLVGLSRKADMYPGQLSGGEQQRVALARAIVNNPALLI
                     ADEPTGNLDPDTSWEIMKLLVDINHRGTTVIVATHEKSIVDSLKKRVIAIDKGLVVRD
                     QQKGTYENEDKNYKTYI"
     gene            complement(6881..7966)
                     /locus_tag="PK733_12760"
     CDS             complement(6881..7966)
                     /locus_tag="PK733_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11445.1"
                     /translation="MSVKLLKDMLNQVNNIIDIECGIMDENGLILACSNEERLGRIDD
                     NIAKVMEIDEQVQIINGISYQRVNANNRYEYVVFLHSHNEESLKYLSLIALNVKNISL
                     YYDDKFDRSNFIKNIMLNNILPGDITIRAKELHVANNINRVALLIRTEGVKDAHPYEV
                     ILSLFPNRSKDYIVVLDEESIVLVKELKSEQANKEIERIAKTIIGTLNTELMVKAMVG
                     IGTVVKNIRDIGRSYKEAQTALLVGGIFENEKSIVYYNNLGIGRLIYQLPTTLCKLFL
                     NEVFRDNSLELLDTETIHTIQKFFENNLNVSETSRQLYVHRNTLVYRLDKIQKMTGLD
                     LRMFDDALIFKFSMMVKRYLDKVEKGT"
     gene            complement(8148..9260)
                     /gene="ugpC"
                     /locus_tag="PK733_12765"
     CDS             complement(8148..9260)
                     /gene="ugpC"
                     /locus_tag="PK733_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOJ11446.1"
                     /translation="MASVVLKNVTKRYEGGVTAVSSFCLDIEDKEFIILVGPSGCGKT
                     TTLRMIAGLEEITEGEVYIGDKLVNDVQPKDRDIAMVFQNYALYPHMTVFDNMAFGLK
                     LRRTPKADIKRRVHEAAKILEIEHLLDRKPKALSGGQRQRVALGRAIVRDPKVFLMDE
                     PLSNLDAKLRVQMRIEISKLHQRLQTTFIYVTHDQTEAMTMGTRIVVMKDGFIQQVDS
                     PQSLYERPVNLFVAGFIGSPQMNFINVKIEKRGEGIYLVFGKHSLKVPEGKAKKISAG
                     DYIGKDVVMGIRPEDVHDEAVYLESMPDSVATANVEVIEMLGSETLLYLFVEEYSITA
                     RVNPRTTARSGDTIKVAFDTNKIHLFDKDTEVVIAN"
     gene            9828..10895
                     /gene="argC"
                     /locus_tag="PK733_12770"
     CDS             9828..10895
                     /gene="argC"
                     /locus_tag="PK733_12770"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOJ11447.1"
                     /translation="MILADSENKKVKVSVIGATGYVGIEIVRILQNHPGVEIASVIGQ
                     SFVGQKISDIYPNLRGLCDIECSSLDIDKLPEQADLFINCLPHGISKDFIPLLLQKGK
                     KIIDHGGDYRYKKVEVYEEWYKTKHELPELLKIAVYGLPELYRDKIRNTQLVANPGCY
                     PTCSILGIAPLLKNALINTDNIIIDAASGVTGAGRKSDLPYQFCECTENFKAYNVSSH
                     RHTSEIEQELSILANKEIKVSFTPHLAPMKRGMLATIYANLKAETTAGELISIYKEFY
                     KGESFIRVLDEGKLPETKHVAGSNFIDIGIVVDKRLNRVIVLSALDNLGKGASGQAVQ
                     DLNIMCGFPEQTALLNPGLYL"
BASE COUNT         3270 a         2286 c         1593 g         3846 t
ORIGIN      
        1 actgctctgc taactgcttg agcgtttcac tatcaaacca atacttcgta ttgcagaaat
       61 gacatacagt ctcagcacca ttctgctcta ttatcatatc ctgtatttct ttttcaccaa
      121 ggctgataat atttctctcc attctatccc ttgaacagtt acatttatat ttacacggag
      181 atttatcagt aattttcaac tccattcctt caaaaataaa ttccagaata tcttccggag
      241 tcttgccatc agttaacatt gatgttacag aaggaatgga ttttatcctg tcatcaattg
      301 catcaattgt tttggtttcg gccccaggca gcaattgtat aataaatcca cctgaatgca
      361 ataccttttc atcagtattc accagtactc ccagagacac tattgaaggc acctgttctg
      421 agtatgcaaa gtaataagca atatcttccc ctatctctcc ggataccagg tctacataac
      481 ctatataggg ctctttcagt ccaagatcct ttatcacatt aaggtaacct tcccctattg
      541 ctcctgccac atcaagctta cctgcactgt ttaaaggtat atatacttca ggattatata
      601 catatcctct aacatttgcc tggtaatcgg atacaactac aacgcctcct aatggtccat
      661 ctccttttat ctgaatggtg ataatattat cctgcgattt taacattttt gacatcattg
      721 cagctgcagt cagagtcctt ccaagagccg ccgatgccac aggagccagt ttatgaatat
      781 tagcagcccg gcttactgta tcggttgtaa ctgctgcaaa agctctgacc tggttcccgg
      841 cagctacaga tcttataata taatcttcca tatatttttc ctccctaata taaaaccccg
      901 gcatagtata catatctaca ccggggttgg atggctactt gaatatattt aaaagtttgt
      961 aagttccctc tttaattaag ccctatgaac gttagctgct tgtggaccct taactccttc
     1021 cacaacttca aactcaactt cagtgccttc ctccaaagtt ttgaacccat ccatgtttat
     1081 tgctgaaaaa tgaacaaata catcatttcc gccttctctt tcaataaagc cgaaaccctt
     1141 ttcagcacta aaccatttta cttttccctt ttccattata aaaatcctcc tgaaataaaa
     1201 cttcagccgg ttaatactac caagccttaa ttagtttatc acactgttaa tcctactgtc
     1261 aactttatat aatcaaaaat tttaattatt tatgtgcaca attattatgt gtaaatatat
     1321 cataaatatt tatataaaaa tttaacatta tggttttcgg catacaaaaa aaagtctctc
     1381 gctttttttc agcggtttat taaaagacaa attgtcatat actactataa gttccaatcc
     1441 gttatcattt atgagtctgc ttatttcttc gactgaataa gccctttcat aatgaatttc
     1501 gtcaaatctt ttatataaat cagacttgtc ttttacgaaa aatgttaaat caaattcgca
     1561 tatttttgtc cttttattat aattatttgc ccatatgtag gtaatatcat cgtttatata
     1621 gtaaaaaaca ttattgttta aaactttttc aaatttatat acagagttta catcaaaaat
     1681 aaacaatcct ccagggttta agtaattcct taccaatttg aacatgcttt taagctctct
     1741 tttgccggta acataattta cactatccat aagtgaaaca aaagcatctg ctgtcccgta
     1801 cagttcaaat tccgacatgt cctgatttaa aaatagaagc ttgtcccctg ctatttctgt
     1861 atcttcagat tttgatactg ccaacgacag catttccgaa gatatatcaa taccaattgt
     1921 atcataaccc cgcttggcca tttcaataca aaagcttcct gtaccacaac ccagatcaac
     1981 aatcaactta ggcttatggt tattgtaatg aaatatcttt tctatataat ctgcccattt
     2041 gcaataatca atatcagaca ttaacctgtc gtaaatataa gccaagtcta aataaataac
     2101 tacaccccct gacaaaagtc cggtggagtg caccttcctc caccggactc aatcctccac
     2161 ttatgcccct taccaatatt ttcttcaaat aaaatacgct ctatatccaa tcatgacctt
     2221 gacaattatt atatgctgcc ttctttacta cctatattat ctgcgccggt ctttttcatc
     2281 cgtttagtaa ccagcccgcc tatacgccct gtttctttgg cagttaggct tttccatccg
     2341 cttttcaaaa ctttgtcaag taacccaagt tctttagcaa tttcatattt tagtctttta
     2401 ttttccttct ccactttctg ccacctccaa gaggtagtat atgaagatat ttctctttta
     2461 tacatttatc tctattaact cgccaatctg tttaatattt caacagcttt cttaagttga
     2521 atatcatcct ctctcggtat ctgggagatt ggcgtatatt tatacttttc atccaggtca
     2581 atatttatat cgggttctat tcctgtacca tgaatacata caccggaagg tgtaaaatat
     2641 cttgatatag ttatttttat accggatccg tcatctaatg gaagcaggtt ttgcactaaa
     2701 ccttttccaa atgttttcgt tccaacaaga gctccctttt tgtgatcttt tattgcaccc
     2761 gccaatattt ctgaagcact ggcgctgttt tcatttataa gaacagcaag gggtatttcc
     2821 agctcccttt tatcggaacg ctcttcttgc acattttttt gtctatcttc agtatataca
     2881 ataagccctt caggaataat tctatcaact atgttcacaa ctttattgta atatcctccg
     2941 ggattatcac gcacatcaat taccaatgct ttaatatttt gatcaagaag ctttttcagg
     3001 tgttcttcaa attctttgtt aattttgtca tccataaagg atattagccg tatatatcca
     3061 atattgtcct gcagtacctc gcttcttata ttaagctgta ctgttatttt ctttcttgag
     3121 acttcaaaat caataggcct gccttcagaa ggtctgtata cagttatttt cactgttgtg
     3181 ttttcaggtc ctttaatcaa tttcactacg gtagactcat cctttaaagc tgttacatct
     3241 tcatcattaa ccttaattat tttatctccc tgtttaattc cagcttcata tgcaggagta
     3301 cctgtaaaag gctccacaac agtaacaatc ccatctttat cagcaattat tgaaactcct
     3361 ataccaacat aagtttcttc agatttcttg gggatttcaa gaatcttatc catttgttcc
     3421 ttggtataat acactgtata tggatcatca agagaagctg ccataccggc taccgcaccc
     3481 tcaagcagca cgttttcatc cacatcttcg tagtaattat cctttaaagc ttttcttgcc
     3541 tgattaaact ttttaatgct ttcatagttt acacttcctg tatcaaagga tatttgatat
     3601 ttgttgttaa aaaccggccc aaggaaaact acggctgcta ttgaaaaggt aataattgaa
     3661 gtgactaaca caatgacaat tataccgata attaccgaac tttttttata catatatttc
     3721 tccccctgta ctgcaatcat ttctctattc tctgctcttc ggactttata tcactgacct
     3781 tattattaat aatatgataa cttgatggta aataacacat ttccatatga tacattttta
     3841 atttttgaag aaatacgttc cagaagctat cattacggta cttagagcct ttaaattcag
     3901 atgcaggcaa aatcaaactc gtaaaataat atgcgcccat ttccatatat caaaatttta
     3961 ttatggttta acataattta acggattttt tgtagctcca tttacccgga cctcaaaatg
     4021 cagatgaggg cctgtagacc atcctgtgct gccaaccttt gctataatct gacctgcttt
     4081 tacttcctgt ccttttttaa ccaatatttt actgcaatga gcataaagag tagtaattcc
     4141 tcctccatga tcaactacta cagtgtatcc ataagcgttc tgccatcctg atattattac
     4201 agtaccttta ttggctgcta aaatgttagc cccactttta cctcctatat caacgcctgt
     4261 atgcattgaa tatgttttaa ggattggatg ttttctgttg ccgtattctg atgttataag
     4321 agatgaactg ggcaggggcc ataccatatt tccttcaaca tatttgcttt ttgatgagag
     4381 ggttattata aggtttccca tttcctcaga tttggctaaa agctcctttt cctgtttttc
     4441 tagtgtagca agtctgctct tgcttgattg aattttttga tcaaggtttg ttcttgaagc
     4501 tttcagagaa tctaatctct gctttttctc ttctattacc ttttgcagtt caactttttg
     4561 ctctgctatt aactgtcttt tacattcaac ttctttcttt gcaagttcaa gctcatccat
     4621 atcgttttta tccttctttg aaagaagaga tataaattcc agtttttcaa gaaaggctgt
     4681 gatgctcttt gattcaaaca acatatcaag gatagtttca ttcgaattct tgtatattgc
     4741 tcttaaacgc gccttaaaac tattacactg cttttcatag ttttgttcag cttcctcaag
     4801 tgctttttct acttctttta aagtcttttc aagctcggca atttccttaa ttaattcatc
     4861 atactcctgg ctttcctttt tttcagtgcc gattacatcc tttttctcct gctcaagctt
     4921 gtttttagca cttaactcat tttttttctg ctggttaatc tggtttatct gagaatcaat
     4981 gtttttcttg tcttgtttgg ccttgtctag ctcatcagca tctgccagca cttgaaaagt
     5041 ccatgttatt atcataatag atattaataa tactgaaatt gcccgtttct tattcataag
     5101 caccacctat aattttctcc tgtaatattt tatctttagc ttatttttaa cttatctact
     5161 tacttttact tgtcttattt ccgtttattt acacttataa gatttctcat tttttattat
     5221 ttataccttt aagtatttac gaattgttgt cacgcttcct gaagcaccaa caatacaccc
     5281 aagaacgata tataatagtg ctaccctggc acttacatca tttagcttaa gtatggatat
     5341 aatacttgtt cctgtcatca ataattcctt tgtaaacctc atttcaacag cattatagcc
     5401 ataccctgat ataagcaagg ctactatgga gcctataaat ccaattataa caccttccac
     5461 tataaaaggc caccgtataa accagtctgt agcacctata tatttcatta tactgatttc
     5521 tcttcgtctt gcaaatactg tcagtttaat agtatttgat ataataaaaa cagaaacaac
     5581 aagcaataca gtagttaata tactattaac tatttgtatt ccatgggtaa actttgaaat
     5641 aaattcaaca gtcttttggg ggtaggtaac cttgtcgact ccatttattt tttcaagttc
     5701 atccattaac atggcactgt cttcaggatt gttaagcttt ataataaatg atacggggag
     5761 aaaactatta tcaattcctt caagcacgct tgcattatcc cccaacatgt cttttacctt
     5821 ctcaaaagcc tgctcttttg ttacaatagc aacctgtttt atcttttcat tttgcattaa
     5881 agcatcctca acaattttca cacccacatc atccagctcc gtgaggcaaa aaacctgcat
     5941 ctccggctga tccttcaatt ttcccacatt atgattgaag tttagaataa tcatatagaa
     6001 tattccaaat attgtaagtg aagccactac tatacttatg gaagccagag acataagttt
     6061 atttctataa acatttgtaa ttccttcttt aaatatatgt tttatagttc ttatcttcat
     6121 tttcgtatgt ccccttttgt tgatctctta ctaccaaccc tttgtcaata gcaataaccc
     6181 tcttttttaa tgaatctaca atactctttt catgggttgc aacaatcact gtagtccctc
     6241 tgtgattaat atcaactaaa agcttcataa tttcccatga tgtatcggga tccaaattcc
     6301 cagtaggttc atccgcaatt aaaagtgccg ggttgtttac tatagctcta gcaagagcta
     6361 cacgctgctg ttcccctcct gataactgtc cgggatacat atctgctttt ctgctaagtc
     6421 caaccagtcc cagagccata ggaacctgtc ttcttatttc ctttggtaaa gactctgtta
     6481 tctgcattgc aaaagccaca ttatcataca ctgttttatc cggcaacaat cggaaatcct
     6541 gaaaaacaac acccaaactc cgcctaagat aaggaatttc acttctgctc atggaagaaa
     6601 cctcatagcc attaacaaaa atttccccct cagtaagatc ttcttcttta agaataagct
     6661 ttatcaatgt cgttttacca gaaccgcttt gtcctacaat aaaaacaaaa tcgcctttat
     6721 taatttttag attaacattg cttaatgcaa gtgttccatt agaatatttc tttgaaacat
     6781 taataaactc aatcattgat aaggtacacc tcacataaca tttaactatt tctacatact
     6841 gatgcggata tctatgtttt tcagtattcg cctaaaactc ctatgtgcct ttctccacct
     6901 tatcaagata ccttttaacc atcatagaaa acttaaatat caacgcatca tcaaacattc
     6961 ttaaatcaag tcctgtcatt ttctgtattt tatccagccg gtaaacaagc gtatttctat
     7021 gaacatatag ctgccgtgat gtttcgctca cattcaggtt attctcgaaa aacttttgta
     7081 ttgtatggat ggtttcagta tcaagaagct caagcgagtt atccctaaac acttcattta
     7141 aaaataattt acagagtgtt gttggtaatt ggtaaatcaa ccttccaatc ccaagattgt
     7201 tatagtacac tatagacttt tcattttcaa aaattccacc caccaacagt gcagtctgtg
     7261 cttctttata tgaacgccct atatctctta tatttttaac cacagtacct atacctacca
     7321 ttgctttaac catcagttca gtatttaggg taccaataat tgtctttgct atcctctcaa
     7381 tttctttatt tgcctgttca gatttcagtt ccttgactaa aacgatgctt tcttcatcca
     7441 aaactacaat ataatccttg cttctatttg ggaaaagact taaaataact tcataaggat
     7501 gagcatcttt aactccttct gtcctaatta aaagcgctac tctgtttatg ttgtttgcta
     7561 catgcagttc cttggcgcgt atagtaatat caccgggcag aatattgtta agcataatat
     7621 tcttaataaa attgcttctg tcaaacttat catcataata caagctaata ttcttaacgt
     7681 tcagagctat aagagataag tatttcaagc tttcctcatt atgagaatgt aaaaaaacaa
     7741 catattcata tctattattt gcattaaccc gttggtacga tatcccgttt ataatctgga
     7801 cctgttcatc aatctccata acttttgcaa tattgtcatc aatccgaccc agtctctcct
     7861 catttgaaca ggccaaaata aggccgtttt catccattat tccacattca atatcaataa
     7921 tattatttac ctgattcaac atatctttaa gtaatttaac tgacaaatac atcactccat
     7981 ataaatacaa tatataacaa ctcaacaggg gacatttcag tatagaggtg tgtcccctta
     8041 ccttatatct tatatcttaa cataaaaaaa ttaaaagagg aagacaaaat atcttcctct
     8101 ttatggaaac ttaataaaat gggatacacc catattcctc aaccttatta atttgcaata
     8161 acaacctctg tatctttatc aaacaagtga attttattag tatcgaatgc tactttaatt
     8221 gtatcacctg atcttgctgt tgttctcggg ttaacccttg cagttatgct atattcctca
     8281 acgaataaat aaagcaatgt ctcagaacca agcatttcaa taacttcgac gttggcagtt
     8341 gctacgctat caggcattga ctccagatat actgcttcat catgtacgtc ttcaggtctt
     8401 atacccatta caacatcttt accaatataa tcacctgcag atattttttt ggctttacct
     8461 tcaggaacct tgaggctgtg ttttccgaat actagataaa tcccttcgcc gcgcttctca
     8521 attttaacgt taataaagtt catttgcggg cttccgataa atcctgctac aaacaggttt
     8581 acaggtcttt cataaagaga ttgaggtgaa tcaacctgct gaataaatcc atctttcata
     8641 acaacaattc tggtacccat tgtcatagct tctgtctgat cgtgggtaac atagataaag
     8701 gttgtttgca gcctttggtg caatttacta atttctattc tcatctgtac ccttaacttg
     8761 gcgtcaaggt tggacaaagg ttcgtccatt aagaatacct tcgggtcacg aactatagca
     8821 cgtcctaatg caactctctg tctctgtccg ccggaaagag cttttggttt tctatcgagt
     8881 agatgttcta tttcaagaat ctttgctgct tcatgtactc ttctcttaat atctgctttt
     8941 ggagttcttc tgagtttaag accgaaagcc atgttatcaa aaactgtcat gtgaggatac
     9001 agggcgtagt tctggaaaac cattgcgata tctctgtctt tgggctgtac atcatttacg
     9061 agtttatccc caatataaac ttcaccttct gtaatttctt ccagacctgc aatcattctc
     9121 aatgtggtag ttttaccaca accagaagga cctacgagaa taataaattc tttgtcctcg
     9181 atgtccagac aaaagctact tacagcagta accccacctt catacctctt agtaacattt
     9241 ttcaatacta cacttgccat tttttcccct cctatattat cattacggat ttgttacggg
     9301 tttgtctctt atttaaatat atcatttact atcattgttc aactagcaca ctttttaaca
     9361 aatataattt ttgtatttgt taaacttgca taatagtgtt aaaaactcta ttgcatttat
     9421 attcattatt atgtataatt ataaattgtg tatgtacata tataacttac gattctaaaa
     9481 agcttatgaa gaattacaaa tcgtaagtta tatcaaggta acagaagcaa gttttaaatg
     9541 cttaaaactt gttctatata agtgctaaaa agtaaaacaa taatataatg atataacaat
     9601 aagagtgtat aataagatta tatactaata ttcgcaaaat gttaatagta cttctatgcc
     9661 aatttggaaa gtgatgtctt ccgggataat ttttgatagc atcgggaaga tgtattactt
     9721 acgggtaagc aaaaattacc ctccagaaat gcgcttccca ttggcaatat aacaaagtag
     9781 taataggttg ttaatgttaa tatgttgttt gtggagggat aaataaaatg attttagctg
     9841 atagtgaaaa taaaaaggta aaggtatctg ttataggtgc aaccggatat gttggaattg
     9901 aaattgtccg tatccttcag aaccatccgg gcgtggaaat tgcctctgtt ataggacaga
     9961 gttttgtagg tcaaaaaatc tccgatatat accctaatct aagaggtttg tgtgatattg
    10021 agtgcagcag tctggatata gataagcttc cggaacaggc tgatttattc attaattgtc
    10081 ttcctcatgg tatatccaag gattttattc ccctgctgct gcaaaaaggc aaaaaaataa
    10141 tagatcacgg gggagattat agatacaaga aagttgaagt ctatgaggaa tggtacaaaa
    10201 caaaacacga actccctgag cttctgaaaa tagccgtata tggtcttccg gaactttata
    10261 gggataaaat acgcaacaca cagttggttg ctaatcccgg ctgctaccct acctgctcca
    10321 ttttgggtat tgcaccttta ctaaagaacg cattaataaa tacagataat ataataattg
    10381 atgcagcctc aggggtgact ggtgcaggaa gaaaatccga tttaccctac cagttctgtg
    10441 agtgtacgga aaatttcaag gcatacaatg tatccagcca caggcatacc tctgaaattg
    10501 aacaggaact gagcatttta gccaataagg aaattaaggt ttcctttacg ccccaccttg
    10561 ctccaatgaa gagagggatg ctggctacca tatatgccaa cttaaaagct gaaacaactg
    10621 ctggtgaact gattagcatt tacaaggaat tttataaagg tgaatccttt attagggttc
    10681 tggatgaagg taaacttccg gaaacaaagc atgttgccgg gtctaatttt attgatatag
    10741 gtattgttgt agacaaaaga cttaacaggg ttatagtgtt gtctgctctg gataatctcg
    10801 gcaaaggagc ttcgggtcag gcggtccagg atttaaatat aatgtgcgga ttccctgagc
    10861 aaactgctct tttaaaccca ggactttatc tataatatat aggtgagaaa tggggtgcag
    10921 gatgtgggaa gtgataagtg agaagtgaga ggtggaatat aggatttgaa atgtgagatg
    10981 tgagaagtgg acaca
//
LOCUS       DAOSDD010000148        10952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2300_length_10952_cov_5.043865, whole genome shotgun sequence.
ACCESSION   DAOSDD010000148 DAOSDD010000000
VERSION     DAOSDD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2300_length_10952_cov_5.043865"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="PK733_12775"
     CDS             <1..1604
                     /locus_tag="PK733_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11448.1"
                     /translation="GKLYNWFRKAEYYSRLIEMNKPVDSKKQIHSNNIYSIFIKHDTL
                     ILDGTVSPKFIESIDRYFYTLENPKSDEKSDNLLAKYYLPENNKETIQRNKQYVLSKI
                     DWLVDRVLSYNFKTNIYIKIFFNASIEEYKNESIRYLIPKIFNNNNYNIETNGTIYGL
                     SNNNMGLNAKKPYLEHKTTNYKVPFRITLDDALEVKSLFEWLESQSENNKPVHYGYIP
                     ISSEDQFSFSSKITDETNSHYIHLEKGIKSIVDDYDFLPGITDKIEPFQLNNYLCLPD
                     FNEEYITSRKTLESKVDDLLYNKNLIKNYNIDKPKIRADFSSRHVDLLNMSKIAMHNY
                     FCKCDDSSIRSCIDRISLALIKESLFICSYKILEKSSVASAINLRLSMLKYFRIGGKE
                     GMGDIVRGLSQIMKEKIIDNKTDEYKQCDSDSEFYFSSGQLTRYLISCSQAERVNYNI
                     IDPILNARDSRKIKNEVMKLIDKYSHALSTKSSRLNNLISMVMGYEPESPDKVLHDIF
                     LAGFASKNLIYYKEKEESKNEKNNE"
     gene            1585..2535
                     /locus_tag="PK733_12780"
     CDS             1585..2535
                     /locus_tag="PK733_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOJ11449.1"
                     /translation="MKKTMNNRVYGVVGIKSIMSNWNADFSGSPKKISTGDIFGSDKA
                     FKYPIKRMWQMQGEKVLYIKSYKISKTGKEDEAEKLQPRDLNERYEQIFGMQLGEKTP
                     SKEVLANLFSAIDVMNFGATFAEKKQSISITGAVQVGQGFNKYDDTNIETQDILSPFR
                     NPTEKKEGSLASSLGSKTVTDEAHYFYPFSVNPSNYVDYVELGIEGFSGYTLEAYEKF
                     KKGCLIAATAFNTNSKIGCENEFALFIVCKENSDLYLANTDRYIDFRKVENRCKISLL
                     KLKEVLKDRLDEIETIEIYCNKLEADVDTCGLPYNVNNLY"
     gene            2547..3299
                     /gene="cas5b"
                     /locus_tag="PK733_12785"
     CDS             2547..3299
                     /gene="cas5b"
                     /locus_tag="PK733_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HOJ11450.1"
                     /translation="MDNNALKFNLSGNAAFYKKPDVNAKIYFTYNNIHKVALLGMLGA
                     VIGLKGYRNNEIFGEKEADYPEFYEKLHALKVSIVPNSERGYFTKKIQYFNNSVGYAS
                     NEEGGNLMIFEQWLENPSWSIYLLNDAGGVEKYLWDKLYDYLITGKSVYIPYLGKNDF
                     PANISDAENVDLEPCTAKFIHSLFIGELDALDDDETIDDDNAFIFTEYAPAALEKGIN
                     FYKFERMIYTNCKVKNSPRHTYKCKDRILSFY"
     gene            3534..5954
                     /gene="cas3"
                     /locus_tag="PK733_12790"
     CDS             3534..5954
                     /gene="cas3"
                     /locus_tag="PK733_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOJ11451.1"
                     /translation="MYNLLNVDNIINSVKIWAHTKKGQPAETLKEHSRLCLDYYNKYC
                     VAKGIDSIVLKIISECGCNEIESKIVYDMFVNAIYLHDIGKINPYFQNRRLNNPEYKN
                     QSTEYEGNSNHALPSAYIYMCEFMQLIEGERKRKLSLFLFSFAYCIAKHHGYLKNTDG
                     FCDDVMYCQVESYYNKILNLKKNSIITTDKGYDKLKKYISSELAFNILCRLLFSVITA
                     CDYCATSEYKNGTKFEISIIENIDKFKSKYVNSNIYKGIMRYKEDKEYFNETPINALR
                     SDMFLEAESQLKENLNGNIYYLEAPTGAGKTNISINLMLNMMKLHPKINNAFYIFPFN
                     TLVEQTYDTLSKYFTKDIDMAVINSVTPIVIKNDKDSEEPDYNYSYLGRIFNNYPIVI
                     TSHVNFFNALFGCGREQVFPLIKLCNSIVIIDEIQSYKNNIWRHIISFLSMYSKLLNI
                     KIIIMSATLPKLDKMLDTERVEFIDLIKNPDRYYQNPLFKDRVDIDLSLLEYEKTDLD
                     VLADKVVQYKDKKVLVEFISKITARNFYNILKEQQDLNVVELTGDDNTRGRKNIIERI
                     GNSDNIIVVATQVIEAGVDIDMDIGFKDISIPDADEQFLGRINRSCLKIGSKVYFFNY
                     DDASKIYKGDLRISYPITNEEIAKMFQCKDFEGIYAKVLTDLKNKTDQLNRDNIKNIF
                     KACLEMKFNEIEKAMRLIDSNIQLFVAHKMVVGNEIIDGRQVWKEFKDLCENDDMEYA
                     EKKVNISLLSEKMSYFSFNIYNINNMQIRCDEEFAGYYYIENGECFIKDGKFDREEFS
                     RAMKGMFL"
     gene            5954..6439
                     /locus_tag="PK733_12795"
     CDS             5954..6439
                     /locus_tag="PK733_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOJ11452.1"
                     /translation="MLTGTIINYFFHCKRQCWLFYNKINLEDNSEDVRIGRILHELKN
                     DGKNEISFENIKLDKITSEYVIEIKKSDADIKAATAQLEFYLMLLLEKGIIRKGRLEV
                     IEKNKQNKKMHYIVLDNIRQEELKAKYKEIEEFLESLIPPQAIYTSKCKRCAYYEYCF
                     I"
     gene            6476..7477
                     /gene="cas1b"
                     /locus_tag="PK733_12800"
     CDS             6476..7477
                     /gene="cas1b"
                     /locus_tag="PK733_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOJ11453.1"
                     /translation="MKEETRYIFSKGNLTRKDFSIQFKNEKGNVYLPIKDTKELYCFN
                     ELSMSTKLLEILSKSGIIVHFFGYYENYIGTFYPKEYLLSGRLTVEQALAYTEKRMEI
                     AKAIVTGIANNIYFVLYHYYRHGKTELKSYLDWLRKDVHSYISKSSDIKRLLSVEGEI
                     WGQFYNTFKTILPEDFSMNKRVKRPPDNPINALVSFGNSLLYTKTITQLYHTHLNQTI
                     SFLHEPSERRFSLSLDLAEAFKPVIVFKTIFDCVNNRKISVEKHFEKKLNYALLNEDG
                     RKVFINEFEERINQTFEHPRLKRRITYKQAIRLDAYKLIKFIMEGKDFTVFDMEKRS"
     gene            7474..7761
                     /gene="cas2"
                     /locus_tag="PK733_12805"
     CDS             7474..7761
                     /gene="cas2"
                     /locus_tag="PK733_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOJ11454.1"
                     /translation="MSKEINYNYAIVFYDIGEDRVQKVFKICKKYFKHHQKSVFRGHI
                     TPANLIAFKAEVSKKINKEYDFITIIQMISQADFKEETLGNGGKNSEDIFI"
     repeat_region   8460..10927
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8460..8489
                     /rpt_unit_seq="gtagaaccgatacatatgatgtattgaaat"
BASE COUNT         4137 a         1427 c         1993 g         3395 t
ORIGIN      
        1 atggtaagct atataactgg ttcagaaaag ctgaatatta cagtcggtta attgaaatga
       61 acaaacctgt agactctaag aagcagatac atagcaataa tatttactca atctttataa
      121 agcacgatac actcatttta gatggcacag tttctcctaa atttatagaa agtatagata
      181 gatactttta tactcttgaa aatcctaaaa gcgatgagaa aagtgacaat ctccttgcta
      241 aatattatct gccggaaaat aacaaggaaa caattcaaag aaacaagcaa tatgtgcttt
      301 ctaaaattga ctggctggtt gatagagttt tatcttataa tttcaaaact aatatttata
      361 ttaaaatatt ctttaatgca agtatagaag agtataaaaa tgaaagcata cgttatttaa
      421 tcccaaagat atttaataat aacaattata atattgagac taacggtaca atatacgggc
      481 tttcaaacaa taatatgggt ttaaatgcca aaaagccata cttggaacat aaaacaacaa
      541 attacaaagt gccttttaga attacattag atgatgcgtt agaggttaaa agcctttttg
      601 aatggctgga aagccagagc gaaaataaca agccagtcca ttatggttat attcctatta
      661 gctctgaaga ccaattctca ttttccagca aaattactga tgagacaaat agtcattata
      721 tccatcttga aaaagggatc aagtctatag tggatgacta tgattttctg cctggaatta
      781 cagacaaaat cgaaccattt caattaaata attatctttg tttacctgac tttaacgaag
      841 aatacattac tagtagaaaa acactagagt caaaggttga tgatttgctt tacaataaga
      901 accttataaa gaattacaat attgataaac ctaagataag ggcagatttt tcttcacgac
      961 atgtagattt gctaaatatg tcaaaaattg ctatgcacaa ttatttttgc aaatgtgatg
     1021 attcttcaat acggagttgt attgacagga tatcactggc actaataaaa gaatcgcttt
     1081 ttatatgtag ctataaaatt cttgaaaaaa gcagtgtggc aagtgcaata aatttaaggt
     1141 tatcaatgct caaatatttt agaataggag gtaaggaagg aatgggagat atagtaaggg
     1201 gtctatccca aattatgaag gaaaaaatca ttgataataa aactgatgag tataagcaat
     1261 gtgacagtga tagtgagttc tatttcagct cggggcaatt aacccgctat ctcatatctt
     1321 gcagccaggc agaaagggta aattataaca taatagatcc aatattgaat gccagggatt
     1381 ctcgtaaaat aaaaaatgaa gttatgaagc tgatagataa atacagccat gcattaagta
     1441 caaaatcatc acgattaaac aacttaattt cgatggttat gggttatgag ccggaaagtc
     1501 ctgataaagt tcttcacgat atttttctag ctggttttgc atctaaaaat ttaatttatt
     1561 ataaagaaaa ggaggagtca aaaaatgaaa aaaacaatga ataatcgagt gtatggtgtt
     1621 gtaggcatta aaagtattat gtcaaattgg aatgctgatt ttagtggaag tccaaaaaaa
     1681 atatcaaccg gtgatatttt tgggagtgat aaagctttta aatacccaat aaaacgaatg
     1741 tggcagatgc agggagagaa agtcttatat ataaaatcgt acaaaataag caagacagga
     1801 aaagaagatg aagcggaaaa actgcagccg agggatttaa atgaaagata tgaacagatt
     1861 tttggtatgc aactaggaga aaagactcca tcaaaggaag ttcttgcaaa cttgttttca
     1921 gcaattgatg ttatgaattt tggagcaaca tttgcagaaa aaaagcaaag tatcagcatt
     1981 acaggtgcag tacaagttgg tcaggggttc aataaatatg atgacactaa tatagaaaca
     2041 caggatatcc tttctccttt tagaaatcca acagaaaaaa aagaagggtc cctggcatct
     2101 tcacttggaa gcaaaacagt tacagatgag gcacattatt tttatccatt ttcagtaaac
     2161 ccttcgaatt atgttgatta cgttgaatta ggtattgaag gatttagtgg atatacttta
     2221 gaagcttatg aaaagtttaa aaaaggttgt ttgattgcag caacagcatt caatacaaac
     2281 agtaaaatag ggtgtgaaaa tgagtttgct ttgtttattg tttgtaaaga aaatagtgat
     2341 ctttatttgg caaatacaga taggtatata gattttagaa aagtagaaaa cagatgtaaa
     2401 attagtcttc ttaaacttaa ggaagtgctt aaagatagac tagatgaaat tgaaacaata
     2461 gaaatatatt gcaataaact ggaagcagat gttgacacct gcggattacc ttataatgta
     2521 aataatttat attaatggtg gctaatatgg ataataacgc attgaaattt aatctttcgg
     2581 gtaatgcggc cttttataaa aagccggatg taaatgctaa aatctacttt acttataata
     2641 atattcacaa ggtagcattg ttagggatgc taggagctgt aataggtcta aagggataca
     2701 gaaataacga aatatttgga gaaaaagagg cagactatcc tgaattttat gaaaagctgc
     2761 atgcattgaa ggtatcaata gtaccaaata gtgaaagagg ttacttcact aaaaaaattc
     2821 aatattttaa taatagtgtt ggatatgctt ccaatgaaga aggcggaaat cttatgatct
     2881 ttgaacaatg gctagagaat ccttcctggt ctatatacct cttaaatgat gctggaggag
     2941 tggagaaata tttatgggac aagctttacg attatttgat aactggcaaa agtgtatata
     3001 tcccttacct tgggaagaat gatttccctg caaatatttc tgatgccgaa aatgtagacc
     3061 ttgaaccatg tacagcaaag tttatacact cactttttat tggggaatta gatgctttgg
     3121 acgatgatga aacgattgat gatgataatg cctttatttt tacggaatat gcacctgcgg
     3181 cactagaaaa gggaataaat ttctataaat ttgaacgtat gatatataca aactgtaaag
     3241 taaaaaattc tcctcgacat acatacaagt gcaaggatag aatcttgagc ttctattaga
     3301 aagaattcta tcaggaaaga taatggggcg gtcaatatga ttattgttga atcgccctgt
     3361 tatacaatct gctaagtcaa tttgaaatag ttatgaataa tataatgttt tatacaaagt
     3421 ttcatcaaaa gatatatcct aaataactta caggcttgat cagtaagaaa taattggaaa
     3481 caaacacttg cgaataaaat tgaagatttt atgcttttgt gaggtgtgat acaatgtata
     3541 atttgctaaa tgttgataac ataattaatt ctgttaagat atgggctcat accaagaaag
     3601 gacagcctgc tgaaacgctg aaggagcata gcaggttgtg cctggattat tataataaat
     3661 attgcgttgc aaaaggaata gattcaatag tgttaaagat catatccgaa tgtggctgta
     3721 atgaaataga gagtaaaata gtttatgata tgtttgtaaa tgctatttac ctgcatgaca
     3781 tagggaaaat aaacccatat tttcaaaaca gacgtttgaa taatcctgaa tataaaaacc
     3841 agagtactga atatgagggt aattcaaatc atgcattgcc ttcagcttac atttatatgt
     3901 gtgaatttat gcaacttatt gaaggagaga gaaaaaggaa gctttctctt tttctgtttt
     3961 cttttgcgta ttgtatagca aaacatcatg gatacttaaa aaatactgat ggcttttgtg
     4021 atgatgtaat gtactgtcag gtagaaagtt actataacaa aatattgaat ctcaagaaga
     4081 atagcattat aacaacggat aaaggttatg ataaactaaa aaagtatata tctagcgaat
     4141 tagcctttaa tatattatgc aggttattat tttcagtaat aactgcttgt gattattgtg
     4201 ctacgtcaga gtataaaaac ggtaccaaat ttgaaataag tataatagaa aatattgata
     4261 aatttaaaag caaatatgta aatagcaata tttataaagg gattatgaga tataaggaag
     4321 ataaagaata ttttaatgag actccaataa atgcacttag gagcgatatg ttcttagaag
     4381 ctgaaagtca acttaaagaa aatctgaatg gtaacatata ttatcttgag gctccgactg
     4441 gagctggtaa aacaaatatt tcaataaact tgatgctaaa tatgatgaaa ttgcacccca
     4501 aaataaacaa tgcattttat attttcccat ttaacacatt agttgaacag acttatgata
     4561 cattaagcaa atacttcact aaggatattg atatggcagt gataaactct gtaactccaa
     4621 tagtaataaa aaatgataaa gactctgaag aacctgatta taattattca tatttaggac
     4681 gaatttttaa taattacccc atcgttatta cttctcatgt aaatttcttt aatgctcttt
     4741 ttggatgtgg ccgagagcaa gtatttccac taattaagtt atgcaatagt atagttatta
     4801 tagatgagat acagagttat aaaaacaata tatggcgtca cattatttca tttttaagca
     4861 tgtattcgaa attactaaat ataaaaatta ttataatgtc tgcaacattg ccaaaacttg
     4921 ataaaatgct tgatacggag agggtagaat ttatagattt aattaagaat ccggataggt
     4981 attatcagaa tccattattt aaagacagag tagatattga tttatcattg ttagaatatg
     5041 aaaaaactga tttggatgtt ttagcagata aggttgttca atataaggat aaaaaagttc
     5101 tggttgaatt tatcagcaag ataactgcaa gaaatttcta taatatttta aaagaacagc
     5161 aggacttgaa cgtagttgag cttactggag atgataatac cagggggaga aagaatatta
     5221 ttgagaggat tggtaactct gataacataa tagtagtcgc aacacaagtt attgaagctg
     5281 gggttgacat cgatatggat attggtttta aggatatatc tattccagat gcagatgaac
     5341 agtttttagg aagaataaat cgttcatgtt tgaaaatagg aagcaaagtg tattttttca
     5401 attatgatga tgcttcaaag atttataaag gtgatttgcg tatcagttat ccaataacta
     5461 atgaagagat tgctaaaatg tttcagtgta aagattttga aggtatttat gcaaaagtgc
     5521 ttacagattt gaaaaataaa acagatcaac ttaatcgtga taatataaaa aatattttta
     5581 aagcatgcct tgaaatgaag tttaatgaaa ttgaaaaagc aatgcgcctt attgactcaa
     5641 atatacagtt gtttgttgct cataaaatgg tagttggtaa tgaaattatt gatggcagac
     5701 aggtatggaa agaatttaag gatctttgtg aaaacgatga catggaatat gccgaaaaaa
     5761 aagtgaacat atctttatta tcagaaaaaa tgtcttattt ttcatttaat atttataaca
     5821 ttaataatat gcaaataaga tgcgatgaag aatttgcagg ctattattat atagaaaacg
     5881 gagaatgctt cataaaagat gggaaatttg atagggaaga attctcacgt gctatgaagg
     5941 ggatgttttt gtagtgctaa cgggaacaat cataaattac ttttttcatt gcaaaaggca
     6001 atgctggtta ttttataaca aaataaattt agaagacaat tctgaggatg tacggatagg
     6061 aaggattcta cacgaattaa aaaatgacgg gaaaaatgag atatcattcg aaaatattaa
     6121 acttgataaa attacttccg aatatgttat tgaaattaag aagtctgatg ctgacattaa
     6181 agcagcaact gctcaacttg aattctacct aatgcttttg ctggaaaaag gaattattag
     6241 aaaaggtaga ctggaggtta ttgaaaaaaa caagcaaaac aaaaaaatgc attatattgt
     6301 tcttgataat ataagacaag aagaattgaa agctaaatat aaagagatag aagagttttt
     6361 agaaagcttg atcccgcccc aggctatcta tacatcaaag tgtaaacggt gtgcgtatta
     6421 tgaatactgt ttcatataaa ttagtagaag aaaataggta caggaggtgt tgaatttgaa
     6481 agaagaaacc agatacattt tttctaaagg aaaccttacc cgtaaagact tttcgataca
     6541 atttaaaaat gaaaagggaa atgtttattt accaattaaa gacacaaagg agctctattg
     6601 ttttaatgaa ttatcaatga gtaccaaact tcttgagata ttatcaaagt ctggaataat
     6661 tgttcacttt tttggatatt atgaaaacta tattggaact ttttatccaa aggagtattt
     6721 gttaagcgga agacttacag ttgaacaagc tttagcttat actgaaaaac gcatggaaat
     6781 tgctaaagca atcgtaaccg gtatagcaaa caatatttat tttgtactat atcattatta
     6841 tagacatggg aaaacagaac taaaatctta tttagattgg ctaagaaaag atgtgcactc
     6901 atatataagt aagagttctg atataaaacg tttattaagt gtggaaggag agatatgggg
     6961 ccaattctac aatactttta aaactattct tcctgaagac ttttctatga ataaacgtgt
     7021 aaaaaggccg cctgataatc ctataaatgc attagtttct tttggaaact ctcttctgta
     7081 tacaaaaact ataacacagc tttatcatac acatttaaac caaacaatat cttttttgca
     7141 tgaaccttca gaaagacggt tttccctaag cctggattta gcagaagctt ttaagcctgt
     7201 aattgttttc aaaaccatat ttgattgtgt taataataga aagattagtg ttgaaaaaca
     7261 cttcgaaaaa aagcttaatt acgcactttt gaatgaggat ggcaggaagg tttttatcaa
     7321 tgaatttgaa gaacgtatta accaaacatt tgagcatccg aggctaaaaa gaagaataac
     7381 gtacaaacag gctattcgtt tagatgctta taagctgatt aaatttatca tggaagggaa
     7441 agactttaca gtctttgata tggagaaaag atcatgagca aagaaataaa ttataattat
     7501 gccattgtat tttatgatat cggagaagat agggtgcaga aggtatttaa aatatgcaaa
     7561 aagtatttca aacatcatca aaagtctgtc tttagaggtc acataactcc tgcaaacctt
     7621 atagcattta aagcggaggt atcaaaaaaa attaataagg agtatgattt tattacaata
     7681 atacaaatga ttagtcaagc agattttaag gaagagacat taggtaatgg tggtaaaaat
     7741 tccgaggata tttttattta gtgtttcagg tacaaaatat attggagttt ttactaaagt
     7801 agaagtaatc tgattttacc attacaatat aagtaatcaa gttataacaa aatgcaaatt
     7861 agttaaacta actttaatca aaaacccctt aaatgtgtaa ataattgtag ccatataatg
     7921 gagggtagat gttattatat ttgctgatga aaaacttaat aattatctta ggcataatag
     7981 ttaactaata catatggtgt attgaaattt tcatttcctt ataggaatga taaaaacaag
     8041 gatactaaac cgatacatat gatgtatttt aataaattaa tttgaatgat tataatttct
     8101 gcagatatct gagtgaataa atttggtata tataagcttg aaattcagct acagtatcat
     8161 tgcagctgaa ctgatatatt tactctatta acagataaat cttcaaaata tgacagaaaa
     8221 agaaatggaa acgagaatta ctatgcgaat taaaaagtga atttgtttga ctttttccaa
     8281 gtacaggtat aaaatcttta ataaaaaggg gcttgaaatt agcttggcta gaggtaaaaa
     8341 cagaaaaaga ttaatggttc tagttacctt tagcgttcac ttggaagaag tcctggcaaa
     8401 tccagatgta atgccagaaa acgtgataat taatattata aaaaccacat ttttcaatgg
     8461 tagaaccgat acatatgatg tattgaaata tagctttaat agatcatatt atcgcaggag
     8521 atacagtaga accgatacat atgatgtatt gaaattaggg agtatgtaaa aataatataa
     8581 aagggagctt tagtagaacc gatacatatg atgtattgaa atttttaaaa tctcctttat
     8641 aattttattt tcttatctgt agaaccgata catatgatgt attgaaataa ataataggat
     8701 catggttatg gattagcggt caaggtagaa ccgatacata tgatgtattg aaataaatgt
     8761 acgctgatgg cataataaaa aatgccggac gtagaaccga tacatatgat gtattgaaat
     8821 cgatacacat ttgaagggcg tgatccttct tatacgtaga accgatacat atgatgtatt
     8881 gaaatttgtc ctgcgctccg tcacctttaa tagcctcttc gcgtagaacc gatacatatg
     8941 atgtattgaa atctcgtaca aatgctctcc ttaataatcc tgttaggcgt agaaccgata
     9001 catatgatgt attgaaataa ccactcgaat cgttaaacat acttggttta tatatgtaga
     9061 accgatacat atgatgtatt gaaatcaggc tatgtctatc atgtcagccc ttatgtgatc
     9121 cgtagaaccg atacatatga tgtattgaaa ttagttaatg cacaaccgtt aaaagttatg
     9181 aacaaggtag aaccgataca tatgatgtat tgaaatgcga gtgagcgtat gcctacatac
     9241 agaattcctg cgtagaaccg atacatatga tgtattgaaa tacgcctttc gcctccctga
     9301 aattcttgca tttccgcgta gaaccgatac atatgatgta ttgaaatacc ccaaccgcta
     9361 tatcgtttac atatattctt ccggtagaac cgatacatat gatgtattga aattggtgtg
     9421 ttacatcttg tatggcctta tcctttataa gtagaaccga tacatatgat gtattgaaat
     9481 agggataaat atatccttaa tgccatttat tataaggtag aaccgataca tatgatgtat
     9541 tgaaatataa ggaccggtcc acaatcgaaa cgatcaatga ccgtagaacc gatacatatg
     9601 atgtattgaa attgtttatt gccatcttct ccgtcctcgc tttctaatgt agaaccgata
     9661 catatgatgt attgaaattt tggtaacatt attgttataa ctggctcttc gttagtagaa
     9721 ccgatacata tgatgtattg aaatcactcg caacaagcca ttcgccagct gcaagttgtc
     9781 gtagaaccga tacatatgat gtattgaaat tgttgcaatt atgcaaatgg tagtaattgc
     9841 tttgcgtaga accgatacat atgatgtatt gaaataacca ctcgaatcgt taaacatact
     9901 tggtttatat atgtagaacc gatacatatg atgtattgaa atgcaatggc agcagaagca
     9961 gaacggaaag aaaaaacagt agaaccgata catatgatgt attgaaatta agcacgtgta
    10021 taatatatcc gtataaaatc tttagtagaa ccgatacata tgatgtattg aaatgtccag
    10081 taaagacccg cctacatact gatattcctt gtagaaccga tacatatgat gtattgaaat
    10141 atgctttatt aggcaggata aaagaaactt ttaacgtaga accgatacat atgatgtatt
    10201 gaaatcggaa tattttgtct acacgttcga acctttgtgc gtagaaccga tacatatgat
    10261 gtattgaaat gcaaaaacgc actcatggct ggcaaaaaga ggttttgtag aaccgataca
    10321 tatgatgtat tgaaataatt ccaatgtcag cttctgtagc tctttttccc agtagaaccg
    10381 atacatatga tgtattgaaa tccatacagt cgtgggttgt gaacttgata atggtacgta
    10441 gaaccgatac atatgatgta ttgaaatcgg acattgaaca tcgatttggc aggcaagggt
    10501 ttgtagaacc gatacatatg atgtattgaa ataaaagaaa tcacacgtac acaagatgtt
    10561 aacaataagt agaaccgata catatgatgt attgaaataa gttaaagcac taaggggctg
    10621 gcgaaacgtt aactgtagaa ccgatacata tgatgtattg aaatataagt gtaccaaaca
    10681 aagtttctgg cgatgtatta gtagaaccga tacatatgat gtattgaaat tatataattc
    10741 ctgattatgc ggatgtaatg ttttagtaga accgatacat atgatgtatt gaaataaaaa
    10801 agctgttttt ggagctttta agaaggtgct gtagaaccga tacatatgat gtattgaaat
    10861 aagattacga tataagtgaa tataatgaat actgcaagta gaaccgatac atatgatgta
    10921 ttgaaatcat gtcagcatgt gctaagataa aa
//
LOCUS       DAOSDD010000149        10915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2310_length_10915_cov_5.034622, whole genome shotgun sequence.
ACCESSION   DAOSDD010000149 DAOSDD010000000
VERSION     DAOSDD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_10915_cov_5.034622"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK733_12810"
     CDS             complement(<1..213)
                     /locus_tag="PK733_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ11455.1"
                     /translation="MYDVACVGILVADAIAKTVDKLPDRGKLDLVDQLDLYTGGCAVN
                     SGIAMAKIGLNIAIIGKIGNDGFGKFL"
     gene            complement(488..3541)
                     /locus_tag="PK733_12815"
     CDS             complement(488..3541)
                     /locus_tag="PK733_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ11456.1"
                     /translation="MLKKESGDIFADIALQRIEYRDDELIQPKPPELPEKYLFADSLN
                     SEAVFRPGKKMNSYEEFAKELKRVKNEYTIYMRDLAPKLESMRTGVTLDRFNWKIETP
                     EDRKEFCNVLEGKEKWEEVRIPHYGEPLGKKATYYRTEFELTDSHFSKGALFIIFKGV
                     DYRAHVFINNSYLGSHEGFFAPFEFEFYDCARKGKNILVVKVENDIPTLKDGDKIYGA
                     TGPGYDDPERGWHHCPPAMGIFQDVFVEARSRLSIKDVFVRPDVENSKAEAWVEIYNC
                     DIEEKEIMLEVSVYGQNFEKKFIENMIYCPQTIQIAGLGDLDKELENKTILKMSAGIN
                     YLRIPYEIDDIYLWSNLTPWLYQLQLKLVYRGKVVDTYSQQFGMRSFKMETQSNPKGR
                     IYLNGQPIRLRGANTMGAMQQSAMRKDWEQLIDDILIAKICNLNFLRFTQRPVQSEIY
                     DYCDRLGMMTQTDLPLFGSLRRNLFCEAIKQAEEMERHVRRHPCNIMISFINEPFPNA
                     NSKPHRNLTRSELESFFEAAGIVVLNCNPDRVIKYIDGDYDPPANCLPDNHCYNLWYN
                     GHGIDIGMLNRGFWQFVKRDWYYGCGEFGAEGLDPVNLMKKKYPSEWLPGSPEEEKEW
                     TPDNILMAQTGKFHYFWYDTPQSLEEWVNASWEHQQFTMKIIAGAFRRDNRMNSFAVH
                     LLIDAFPSGWMKALVDYERTPKPAYYTYRDMLTPLMTNLRADRCSYYDDERINIEAWI
                     CNDMNTKPENAYICWFIELREQILYSGKTKADIRLNCSTFQGYISFQAPHVDERESIN
                     VRFGLLDDNNVLHDTCIQLNVFSSSIKEHNLKVCIVGNKEGSAVELTKELRAAIESLE
                     NDKKQIILIQDFKSYKEKSDTIDMAVENGSSAIFITLEPGEHIVSGKHIKVVPCTMGP
                     RHFVSRATGHPLVKDFKDNDFKFWYDSSKDMITPILDAVFCSEDFIPVLVSGNKDNNS
                     KYSSFMVVGERKYGKGRFIICQLKLENKLNGNPAARIFANRLLSL"
     gene            complement(3666..4739)
                     /locus_tag="PK733_12820"
     CDS             complement(3666..4739)
                     /locus_tag="PK733_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11457.1"
                     /translation="MTNNKNILLSEYAPKSELVVKEHEVRKPRFNAIDFHIHLRYMLK
                     EYNNKIDYDIKNVVEEFKSFGIKHIVNLDGFPELLDRMLGKTGQYNDNFITTFGRLDF
                     TKIDNPDFDKYVKQILKDFKSKGIKGLKVWKDLNLKIKDKQGKCIPPDDERLRVVWET
                     AADLGLVVLTHIADPKAFFKPININNERFEELNRRPDWSFYGPEYFEFEELMEMQYNM
                     VSKNSDTIFVLSHVASSSENLGYVSECMDLCSNMYVDIAGRISELGRQPYSARKFFNK
                     YQDRILFGTDSTPLGNNYLRNYRFLETWDEYFDYEDSIVPRRGRWEIYGIGLEEHVLE
                     KIYFKNAEKLLGLANYRVKKPAN"
     gene            complement(4946..10702)
                     /locus_tag="PK733_12825"
     CDS             complement(4946..10702)
                     /locus_tag="PK733_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11458.1"
                     /translation="MSVSINVNDVIRIEGTSENNDCAYLDKIELTSTSIPPEPIVIVM
                     EAESMTLSNLSKEKNTPTNTIVVRPPWGQTGYAEGTFTGDNGTYDAKIYYFDENDGKA
                     IYKLTVGDTVIQTWEADVDLGSPNPDTDTYTFKQITGISIISNDTIRVDVTGNGSDNG
                     RFDKIEFTLSPSTEEKPIPVSDVYYVDPINGDDDNDGTFELPFATIEYVRDVIRQVIA
                     DGMTSDITVYLRGGAYNLTSSLEFNSDDSGRDGYKIIYKNYNDETPIINGGFAVTGWE
                     LHEGSIYKTNVGDNRFSTLYENGVRSVIARYPNSGYNKAIAANPLSKSQFVFDSDKGD
                     IPVVANPSGLEVLMWSGAYNWSADTVRVSSINYESSLVTLASGCTYTLDTGSRYYVQG
                     AMELLDQPGEFYLDSSTGTLYYWPRQTSIEDQEIIAPMNDDIIKFIGNSGEDPVDSIV
                     LEGITIRNSDRGKNAFSLKNASNIVVKDCKIHNTGGNAIDIKGFAQFNVIEGNLIYDI
                     GSSGIMVTGYNGAVATIYHVSKNNNIVNNHIYDTGSITGAGYGIYIRQSGDNLVSHNL
                     IHDTTRFAIAADTNARSGKTLGKKFDGVLVTTDNYRDFIHTSGNIIEYNDVSRANTDS
                     QDTGVIYSNGTNMGNILRNNYIHDSSVPFGSGYGIYLDDAADGWIVEKNIVSDFTVSG
                     GGSLSSTIFAKGIGNKFTNNIIANNATAIISRMRSQFNEPNYDLEYKNNVTYNTGENI
                     YYFYSDYKDDKIKYSDYNLHYNDDEVYNIGGGTPCNSFELWKMEQNNRYDSHSLTTDP
                     IFMDADNSDYRLKYDSPAYLLGVEDIDIQSIGLTEQFKYSDVNEDIGRVFVRKSGSDV
                     NKSFINLDVSETANLEVTARTVTGYLANLSGAAITYSSDDDSVASVDNNGTVTANNPG
                     VALISVVVEKGSVSETVQMHVIVGSEISEVFLEAQKTWANVDEQMQFNVYGKSDYNQH
                     ISSDDYVIEYASSDESIATIDNNGLFKACKVGVVTITVNVKKNEDVYSKDIIIYVVDN
                     TLDSVTATADSNKLYIGDTTQLSVEGIMSDDSLFDINEAEISFTSADESMATVDNSGK
                     VTATGTGNAIIITNVNYKGITKITTTEIYIKGDPEQPWDGVENAFGGWTMKKYGNADG
                     ISVFDNGKLTIASSGFDIFGNTDDFVYAYKEIECTESTASISATIESLENTHSSAASG
                     VMFREKVSAADENNGAGAKNIIIRIQPNGSILMTYRTSSDWSTAYKSGPVLTFPAELK
                     IEKEGNMYSCYYKTSHQWSKLGEIEIDMGNNILAGVAVFSRKENVSTSSQVSGIKINP
                     DTSASEIVSVEQLSNITVNYGTAFKDINLPKKVEVTLDDERMIKLPVTWSAEGYDGLV
                     AGSKKITGTFAILPEGLENSAEKTAEITVVVKEKVDETEHSDINTGTPTTTEESKLNE
                     IVEVAALDSIIVDYGTVFKDISLPENVEVTLDDGRKIMLPVMWSSEGYNGFISGSRAI
                     SGTFASLQGNISNSKGYVANITITVTVNSSVIVTVGKDDKRELSSAIVENNDGNIILR
                     LDENSMKDIEGTTSKAIDNIRLEIPLHEGANQYGLELPTGTLTHGEMNKHIEVVTGFG
                     VISIPNNMLTNTLLKRSEKVEISITNIDSSTLGADLSSMIGERPIVQISISADGKVVN
                     WNNTDAPVTVSLPYVPTPDELENPEHIVVWYIDGNGSIVPVTNGRYNPDTGMVTFTTT
                     HFSKYAVAYVKKSFDDLDKFEWSKKAVEVLASKGIIKGTSEKTFAPDLYITRADFIVL
                     LVKTLDFNTSFSSNFSDVSSDKYYYNELGIAKKLGIAMGVGDNQFKPQAYISREDAMT
                     LAQRALKLAGKLKTGGTTEDLKGFDDRGKVASYAVESMAALVKEGLVKGDGKMLNPKS
                     NAMRAEMATFLYRIYNLK"
BASE COUNT         3236 a         2320 c         1539 g         3820 t
ORIGIN      
        1 aaggaatttc ccaaatccat cattgccgat tttcccgata atagctatat taagaccaat
       61 tttagccata gctattccag aattcacagc acatcccccg gtatataaat ccaactggtc
      121 caccaagtca agtttccctc tatcaggaag tttatcaact gtctttgcta ttgcatcagc
      181 cactaaaata ccgacacatg ctacatcgta cataactacc acccctttat tatcttttat
      241 ggtgttataa gatagtttca tcttattata atgttgtaat ttaattaatc ctattttacc
      301 acaaagcaga gggtaagttc cagtaccaga tacaaatggg cgcaaattat atttatgaag
      361 aaattacacc catcggtact tattcatctt ctttaaatac cggcatatac tttaatctaa
      421 attcaaacta ttttcaatta gcgctatgct tctccgcgtt aatctatgtt atataaataa
      481 atcactttta taatgataaa agcctattag caaaaattct tgctgcagga ttgccattca
      541 gcttgttttc aagttttagc tgacatataa tgaacctgcc ttttccatat tttctttctc
      601 caacaaccat aaagctggaa tatttactat tattatcttt attaccgctt acaaggacag
      661 ggatgaaatc ttcagagcaa aataccgcat ccagaattgg cgtaatcatg tctttagaag
      721 agtcatacca aaacttaaaa tcattatctt taaaatcttt tacaagggga tgtcccgttg
      781 cccgagaaac aaaatgtctt ggtcccattg tgcatggaac aacctttatg tgtttccctg
      841 agacaatatg ctcaccaggt tctaaggtaa taaatattgc actacttcca ttctccactg
      901 ccatatcgat agtatcactt ttttctttat aactcttaaa atcctgtata agaataatct
      961 gttttttatc attttctaaa ctttctatag ctgccctcaa ttctttagta agttcaactg
     1021 ctgatccttc tttatttcca actatacata ctttcagatt atgttcttta atgcttgaag
     1081 aaaaaacatt caactgaatg caggtatcat gtagcacatt attatcatct aaaagaccga
     1141 acctcacatt tatgctttct ctttcatcta catggggagc ttgaaaagaa atataaccct
     1201 ggaaagttga acagtttaac ctaatatccg ccttggtttt tccagagtat agaatttgtt
     1261 ctcttaattc tataaaccaa catatatagg cgttttcagg tttagtattc atatcattac
     1321 aaatccaggc ttcaatattt atacgttcat catcataata tgagcatctg tctgctctta
     1381 aattagtcat aagaggagta agcatatctc tatatgtata gtatgcaggt ttgggtgttc
     1441 tttcatagtc taccagagcc ttcatccaac ctgaaggaaa tgcatctatt aaaagatgaa
     1501 cagcaaaact attcattctg ttatcccttc taaatgctcc agcaattatt ttcattgtaa
     1561 actgttgatg ctcccagctg gcattaaccc attcctctaa tgactgaggt gtatcatacc
     1621 agaaataatg aaactttcct gtttgagcca ttaaaatatt atccggagtc cattcttttt
     1681 cctcctccgg tgaaccggga agccattcag aaggatattt cttcttcata agatttaccg
     1741 gatccaaacc ttcagcacca aattctccgc aaccataata ccagtccctt ttaacaaatt
     1801 gccaaaatcc tctattcagc atgcctatat caataccatg tccattatac cagagattat
     1861 agcagtgatt atcaggcaga caattagccg gtgggtcgta atctccatca atatatttaa
     1921 tcactctatc agggttacag ttaagtacaa caataccggc agcctcaaaa aaagattcaa
     1981 gttcacttct tgttaaattt ctatgaggtt tggaatttgc attaggaaag ggctcattga
     2041 taaatgatat cattatatta caggggtgtc ttcttacatg cctttccatc tcctcggcct
     2101 gttttatcgc ttcacaaaac aagttcctac gtaagcttcc aaaaaggggt aaatccgtct
     2161 gagtcatcat tccaagacga tcacagtaat catatatttc agactgcact ggtctttgtg
     2221 taaatcttag aaaattcaag ttgcatattt ttgcaataag gatatcatca atcaattgtt
     2281 cccaatcctt tctcatcgca ctttgttgca tagctcccat agtatttgca cctcttagac
     2341 ggattggctg tccatttaaa tatattctac ccttcgggtt actctgagtt tccattttaa
     2401 atgaacgcat accaaattgt tgactatatg tgtcaactac tttgcccctg tataccagtt
     2461 taagctgcaa ttggtataac catggtgtta aatttgacca caaatatata tcatctattt
     2521 cataaggtat acgaaggtag tttatacctg cactcatttt aagaattgtc ttattttcca
     2581 gttccttatc tagatcccct aatcctgcta tctgaattgt ctgtggacag taaatcatat
     2641 tctcaataaa ttttttttca aaattctgtc cataaacaga aacttcaagc ataatctctt
     2701 tttcttcaat gtcacaatta tatatctcaa cccatgcctc agccttacta ttttcaacat
     2761 ccggtcttac gaaaacatct ttaatgctta accgtgacct tgcttccaca aatacatcct
     2821 gaaatatacc catagccgga ggacagtggt gccatcctct ttcaggatca tcatatcccg
     2881 gacctgttgc accatatatt ttatccccat ctttcaaggt tggtatatca ttttcaactt
     2941 ttacaaccag tatatttttc ccttttcttg cgcagtcgta aaattcgaac tcaaatggag
     3001 caaaaaaacc ttcatgagac cctagatatg aattattaat aaatacatgt gctctatagt
     3061 caactccttt aaaaattata aatagagctc cctttgagaa atgcgaatct gtaagttcaa
     3121 attcagtcct ataatatgta gctttctttc caagaggttc accgtagtgt ggaattctaa
     3181 cttcttccca tttttctttt ccctccagca cattgcagaa ctcttttcta tcttccggtg
     3241 tctcaatctt ccaattaaat ctatccaaag taacccctgt cctcatagat tctaatttag
     3301 gtgccagatc tctcatatat attgtatatt cgttttttac cctttttagc tctttggcaa
     3361 actcctcata gctattcatt tttttaccag gcctgaaaac tgcttcagag tttaacgaat
     3421 ctgcaaagag atacttttca ggcagttcag gtggttttgg ttgtatgagc tcatcatctc
     3481 tatattcaat tctttgtaag gcaatatctg caaaaatatc accagattct tttttcagca
     3541 aaagtttcca cctctttttt taaattgtaa agctatcaac atgattaata taacaatatt
     3601 aggtgttttc aaaaaatata tacactctat ctagtcatac gaatattagt aaacttcttg
     3661 aaatatcaat ttgctggttt ttttactcta tagtttgcta atccaagcag tttttcagca
     3721 tttttaaagt atatcttctc cagcacatgt tcctctaatc ctataccata aatttcccat
     3781 ctaccccttc taggaacaat actgtcctca taatcaaagt attcatccca agtttcaagg
     3841 aaacggtaat ttcgaagata attattcccc aatggggtag aatctgtacc gaataatatt
     3901 ctatcctgat acttattgaa aaactttctt gctgaataag gctgtctgcc taattcagaa
     3961 attctgccag caatatcaac atacatgttg gaacataaat ccatgcactc tgatacatat
     4021 cccaggtttt cggaacttga agcaacatgt gataatacaa aaatagtgtc agaattcttg
     4081 ctaaccatat tatattgcat ttccataagt tcctcaaatt caaaatactc aggcccatag
     4141 aaagaccaat caggtcttct gttcaattcc tcaaaacgct cgttatttat attaataggc
     4201 ttaaaaaaag cctttggatc agcaatatgt gttaaaacta ccaaacctaa atctgctgct
     4261 gtttcccata caacccttag cctttcgtca tccggtggta tgcatttccc ttgtttatct
     4321 tttattttaa gattcaaatc cttccaaact tttaaccctt taataccttt tgacttaaaa
     4381 tcttttaata tttgtttaac gtatttgtca aaatccggat tgtctatttt ggtaaaatcc
     4441 agccttccaa atgtagtaat aaaattatca ttatattgcc ccgtttttcc cagcatacga
     4501 tcaagtaact ctggaaagcc atctaaatta acaatatgtt ttataccaaa gcttttgaat
     4561 tcttcaacta cattcttaat atcatagtct attttattgt tatattcttt caacatatat
     4621 cgtaaatgga tatgaaaatc tattgcatta aatcgaggct tcctaacttc atgctctttt
     4681 accactaatt cactcttagg agcatattct gaaagcagaa tatttttatt gtttgtcata
     4741 gttaattacc aaccttgttt tttttaatat agcaatctta aaaagatcca ctaaaatatt
     4801 taaatatcct gtacaattta cgatttgaaa aagctaatga agaattgtaa atcgtaagtt
     4861 atatcaaggt aacagaacag gttttaaatg ttttaaaacc tgttctatac agtgaacctt
     4921 tttgtttatt aattcatcta atttatcact taaggttata tattctgtaa aggaatgtcg
     4981 ccatctctgc acgcattgca ttactcttag gattaagcat tttaccatcc ccttttacca
     5041 gaccttcttt tacaagtgca gccatgcttt ctactgcata agatgcaact ttccccctgt
     5101 catcaaaacc tttcagatct tctgtagtac ctccagtttt aagctttcca gccagtttca
     5161 atgctctttg ggcaagtgtc atagcatctt ctctagagat atatgcttga ggtttaaatt
     5221 ggttatctcc aacacccatt gcaattccaa gcttctttgc tataccaagc tcgttgtaat
     5281 agtacttatc ggaagataca tcactgaagt ttgagctaaa cgatgtatta aaatcaagag
     5341 ttttaaccaa aagtacaata aagtcagctc ttgtaatgta taaatcaggt gcaaatgttt
     5401 tttcagaagt acctttaatg attcccttag aagcaagtac ttctactgct tttttactcc
     5461 attcaaactt atcaaggtca tcaaaagatt tctttacata agcaactgca tacttactga
     5521 agtgcgttgt agtaaatgtt accattccag tatccgggtt atatcttcca ttggttacag
     5581 gtacaatgct accatttccg tcaatatacc atactacaat gtgttcagga ttctccagct
     5641 catctggtgt tggtacataa ggcagtgaca ctgttactgg tgcatctgta ttgttccaat
     5701 taaccacctt tccatctgct gaaatactaa tctgtactat tggtctttcc cctatcatac
     5761 tgctaagatc agcgccaaga gttgaactat caatattagt tatactaatt tcaacctttt
     5821 cgcttcgttt caataaggta tttgttagca tattatttgg aattgaaatt actccaaatc
     5881 ctgtaacaac ttcaatatgt ttgttcattt caccatgtgt caatgtccca gtgggaagtt
     5941 ctaagccata ttgatttgca ccttcatgaa gaggtatctc cagcctgata ttatcaatag
     6001 ctttgcttgt agttccctct atatccttca tactgttttc atcaagtctt aatattatat
     6061 taccatcatt attttctaca atagctgaag acaattcccg cttgtcatcc ttgccaaccg
     6121 ttactattac actgctgttt actgtaactg taatagtaat attcgctaca tatcctttgc
     6181 tattggagat atttccctga agacttgcaa atgtcccgct tattgccctg ctaccagaaa
     6241 taaaaccatt ataaccttct gaagaccaca ttactggaag cattatcttt cttccgtcat
     6301 ccagggttac ttccacattt tcaggtaaac ttatatcctt gaagactgta ccataatcaa
     6361 ctattatgct atctaatgca gcaacttcta ctatttcatt aagcttactc tcttcggtag
     6421 tagtgggagt acctgtattt atatcactat gttctgtttc atcaactttc tctttcacaa
     6481 caactgtaat ttctgctgtt ttctctgcac tattttcaag accctctgga agaattgcaa
     6541 atgtcccggt aattttcttg ctgccagcaa ctagaccatc ataaccttct gcagaccatg
     6601 ttacaggaag cttaatcatt ctttcgtcat ccagggttac ttccactttt ttcggtaagt
     6661 ttatatcctt gaaagctgta ccatagttaa ctgttatgtt gctcagttgt tcaactgata
     6721 ctatttcact ggcacttgtg tcaggattta ttttaatgcc agacacttgt gatgatgtgg
     6781 atacattttc cttccttgaa aatacagcta ccccagctaa tatgttatta cccatatcaa
     6841 tttcgatttc tcccaactta ctccattggt ggctagtctt gtaatagcat gaatacatat
     6901 tgccttcctt ttcaattttt aactctgcag gaaatgttag cacaggacct gatttataag
     6961 ctgttgacca atctgaacta gtacgatatg tcattaaaat actaccattt ggttgtatac
     7021 gtattattat attctttgca ccagcaccat tgttttcatc ggctgcactg actttctccc
     7081 taaacatgac acccgaggca gcactgctat gtgtattttc aagagattca attgttgcac
     7141 taatagatgc tgtgctttca gtacattcaa tttccttata tgcatataca aaatcatcag
     7201 tgttaccgaa tatatcaaaa ccgctgcttg caatagtcaa tttaccatta tcgaaaacac
     7261 taataccatc tgcattgcca tattttttca ttgtccatcc gccaaaagcg ttttcaacac
     7321 catcccatgg ctgctcagga tcgcctttta tataaatttc tgttgttgtt attttggtaa
     7381 ttcccttata gtttacatta gttattatta tagcattacc tgttccagtc gctgtaactt
     7441 ttccgctatt atctacggtg gccatgcttt catctgctga agtaaagcta atttcagcct
     7501 cattaatatc aaatagggaa tcatcactca ttattccttc taccgatagc tgggtggtat
     7561 ctccaatata cagcttatta gaatcggcag tagcagtaac tgaatcaagt gtattatcaa
     7621 caacataaat tataatgtct ttactgtaaa catcttcatt tttcttaaca tttacagtaa
     7681 tagtcacaac accaaccttg caggctttga aaagaccatt attatcaata gtggcaatgc
     7741 tttcatcact gctggcatat tcaataacat agtcatccga gcttatatgc tgattatagt
     7801 ctgattttcc gtatacatta aactgcattt gctcatctac atttgcccat gttttctgtg
     7861 cttccagaaa tacttccgat atttcactac caactattac atgcatttgt acagtctctg
     7921 atacactgcc cttttctact actacactaa ttaaagcaac accagggtta ttcgctgtta
     7981 ctgttccatt attgtccaca gatgccacag aatcatcatc acttgaataa gtaatagctg
     8041 cacctgaaag gtttgctaaa tagccggtaa ctgtccttgc tgtaacttca agatttgctg
     8101 tttctgatac atcaaggttt ataaaggatt tattaacatc gctgccagat tttcttacga
     8161 acactcttcc aatatcttca ttaacgtcac tatatttgaa ttgctctgta agtcctatac
     8221 tctggatgtc tatatcttct acccctagta agtatgccgg agaatcatat ttaagtctat
     8281 aatcggagtt atctgcatcc ataaatattg gatcggttgt aagagaatgt gaatcatacc
     8341 tgttgttttg ctccattttc cataactcaa agctattaca tggagttcct ccaccaatat
     8401 tatatacctc atcatcgtta taatgtaagt tgtaatctga atattttatc ttatcgtcct
     8461 tataatctga atagaagtaa taaatatttt cacctgtatt ataggtaaca ttgtttttgt
     8521 attcaaggtc atagtttggt tcattaaact gtgaacgcat tcttgatatt attgctgtag
     8581 cattgtttgc tattatgttg tttgtaaatt tattaccaat acctttagca aatattgtac
     8641 tcgataagga acctcctcct gaaacggtaa aatcactgac aatatttttt tctacaatcc
     8701 acccatcagc tgcatcatcg agatatatcc catatccgga accaaagggt acactactgt
     8761 catgaatata attgttcctt aaaatatttc ccatatttgt tccatttgaa tatattacac
     8821 ctgtatcttg agaatctgta tttgcacgtg acacatcatt gtattcaata atattaccgg
     8881 aagtatggat aaaatcacgg taattatcag ttgttaccag tacaccatca aattttttgc
     8941 ctaaagtctt gcccgacctg gcatttgtat cagcggcaat ggcaaagcgg gtagtatcat
     9001 ggataaggtt gtgtgaaacc aagttatcac cactctgacg aatgtatata ccgtaacctg
     9061 ctccagtaat actgccagta tcataaatgt ggttattgac tatattatta ttcttgctta
     9121 catgataaat agttgcaaca gctccattgt aacctgtgac cataatgcct gagctaccta
     9181 tatcatagat caagttcccc tcaatcacat taaattgagc aaaaccttta atatcaatag
     9241 cattgccacc tgtgttatgt attttacaat cttttactac tatatttgat gcatttttca
     9301 atgaaaaagc atttttacct ctatccgagt tcctgattgt aataccttca agcacaatac
     9361 tatctaccgg gtcttctcct gaatttccaa taaacttaat aatatcatca ttcataggtg
     9421 ctattatttc ctgatcttca attgatgttt gacgtggcca ataataaagg gtacccgttg
     9481 aagaatcaag gtaaaactcc ccaggctgat caagaagctc catggccccc tgtacataat
     9541 accttgatcc ggtatccaaa gtataagtac agccagaagc cagagtaacc agactgcttt
     9601 cataattaat tgatgataca cgaacagtgt ctgcagacca gttgtaagca cctgaccaca
     9661 ttaagacctc cagtccagac ggatttgcaa ctacaggtat atcacccttg tcactatcga
     9721 atacaaactg tgattttgaa agtggatttg ctgcaatagc tttgttgtag cctgaattgg
     9781 gatatcttgc tataacgctg cggactccat tctcatataa ggtagaaaat ctattgtcac
     9841 ctacatttgt cttataaata ctgccttcat gcaactccca tccagtcacc gcaaaaccgc
     9901 cgttaataat cggtgtttcg tcgttgtagt tcttatatat gattttatat ccgtccctac
     9961 cactatcatc tgaattaaat tctaatgaag aagttaagtt atatgcacca cctcgcaagt
    10021 aaacagtaat atctgaggtc atgccatccg caattacctg ccttataaca tctcgtacat
    10081 attcaattgt agcaaatggt agctcaaagg taccatcatt atcatcgtcc ccatttatag
    10141 gatctacata atatacatct gaaacaggta tcggcttttc ttctgtagaa ggtgaaagag
    10201 taaattcaat tttatcaaaa cggccattgt cacttccatt accagtcaca tcaactctga
    10261 tggtatcatt gcttataatg ctaatacctg ttatttgctt aaaggtataa gtatctgtat
    10321 ctggattggg tgaaccaaga tcaacatcag cttcccatgt ttgtattaca gtatctccaa
    10381 cagtcaattt atatattgct tttccatcat tttcatcgaa atagtatatt tttgcatcat
    10441 atgtgccgtt atcaccagta aatgttcctt cggcatagcc agtttgtccc catgggggcc
    10501 ttactacaat tgtattagtt ggagtgtttt tctccttact tagattgctc aaagtcatgc
    10561 tttctgcttc cataacgatc acaatcggtt caggaggaat tgatgtgctt gtaagctcta
    10621 tcttatcgag ataagcacag tcattgtttt ccgatgttcc ttctattctt ataacatcat
    10681 ttacatttat acttaccgac ataatttcac gcatagttaa agtttgagca tttatttcag
    10741 aagaacctaa attctggtca gctgtccaac tgtcaataac taccccacct acaagcagcc
    10801 ggaaaattgc ttttccatca tttaaatcgt aataatgtac ttttatatta tattcatctg
    10861 catccctgta aacacatgct ctacatatcc cgaactgccc tcagtcaatc ttata
//
LOCUS       DAOSDD010000150        10910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2312_length_10910_cov_5.369691, whole genome shotgun sequence.
ACCESSION   DAOSDD010000150 DAOSDD010000000
VERSION     DAOSDD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_10910_cov_5.369691"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2534)
                     /locus_tag="PK733_12830"
     CDS             complement(<1..2534)
                     /locus_tag="PK733_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ11459.1"
                     /translation="MKDGENNHIHNAADGERSALIGFVPQYKIAAKLIYDALLKGTFE
                     WIGIMDPEAEKLDDIQIATQGRLDAYQVKWGEFAGAKTFNDIIKPQKSKNKVIKPPLI
                     MQLAEGWEAMTRIHTDRIINVHLVSNDIPSTNDTIVSDDEPPRYKHFQAFLRDCWDDK
                     NWIKDGFSVIPKGWKAAINTIKDVTCFDEEKFLLFVKDCYFDFNYQIFEPEDQESVTL
                     QRRKDKNEIFKFLMNAAGGQKRILKITCQELLKSLGWENRFKFRFPHEFWVDEKTYQP
                     ILSTINDIEQSLNSFNNGYLALIGTPGSGKSTTLTQTFRYRKGYRVIRYYAYIKDDTR
                     LGRGESLNFLHDLVLALKNHGIRGKGKAFPESREELLEVVGSQLSELRSMWQVDGIRT
                     IIIVDGLDHIEREQSPERTLLMDLPNPEAIPEGTLFILGTQKIDLAGLSPNIQTQLQQ
                     EGRTLTMNPLGRTDVFNVIDSIEFPIELTNIQKDTIFKLSDGHPLALHYLLQKLKTYR
                     MHDEIDTILNSSNPYTGHIDQAYEVYWKSISSDKDIRELLALISRLRGTIDLIEVEKW
                     FGLTIVERFIIQTKHFFRVDNFTRWHFFHNSFRQFILQKTERDLLGRDQNSHFHKQLA
                     HFASNNDADVEWSWEKLYHYSCAGEDKKTIEIGQQDYFREQYYSLRPLDDIIDDITLC
                     LKAAAKENDGLCVIRQFLIEKELRERDENINDVDLPRLLLELNGVDAAIKHIIHGKEL
                     RTSQAKALEFCSLLIEKGAYESARNIFNVAEPVELLNGSQGVDTIKDGNIQAVKKWAE
                     VAHYFRPLTQVFRAIEQLRGDNRRYNYYEDPEEEKHHIQSSVLRVLS"
     gene            complement(2876..3277)
                     /locus_tag="PK733_12835"
     CDS             complement(2876..3277)
                     /locus_tag="PK733_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11460.1"
                     /translation="MEENFIDYVRIILNFYSSLGKEKVFNDNRYCNGNSDAMVYSRLE
                     RVAFNQLDDNASATYSRYAIWAADVREIINEVIKSIDNNELDDAKGKMILALNAMGAY
                     IDIQSLFDSQPGGMQFDSPEDIIKIYDNYKI"
     gene            complement(3328..3531)
                     /locus_tag="PK733_12840"
     CDS             complement(3328..3531)
                     /locus_tag="PK733_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOJ11461.1"
                     /translation="MIKICYICNTEMEKKTVPINTGWGQYKLTVEDVSSYVCPKCGEV
                     VLKGKDAIMLQKLSKSLAGAGDK"
     gene            complement(3593..8659)
                     /locus_tag="PK733_12845"
     CDS             complement(3593..8659)
                     /locus_tag="PK733_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOJ11462.1"
                     /translation="MAQINNIFSFLKDYNELSNPVITEIDKQKWIMRFSDLPQIKELW
                     SIYTVQDFNELKILEIERPTLEPCPAPDKSILDWLEGNWKKLNVENIQYKEVLVKEAL
                     NEDDKVDQVEEFFIDDETRKILFDEWIKKRNNWRAVEIPKEQGLTLYNNLFRLYSDMK
                     KESEGVELILGDGHIRWSMADRIIDHPVLLQRVQLEFDSEKPSFIIKCEELKTELYTP
                     MLRIIPTINQKMLSDVLQDIEANAYHIADTANILGLFQRLINVVDEKGKCVEVFENGY
                     NGPMIMSAPTLFLRKRTLGFSVFIEKIIEELKENKDITLPAFFKNMVGNYKEHTDIDV
                     IEDNWNQSGIDQDVLLTLPANNEQLKIIKYLNSYGAVLVQGPPGTGKTHTIANLIGHL
                     LSQGCSVLVTSHTEKALTVLKEKVYKDQYNKDINLQSLCISLLSSNSQKKEMDDAINE
                     IAIKSTSTDLFESKKKIERLENERKELISKSKAKSQELLYIRSLEYKDIIFDNHTISP
                     IDAAKFINTGYGNFDYIPGSTSDDRIGLPLSTENLDFLYHSNQQISLEEDELLRKKLP
                     SLASLWSIDSFRINTENYIKYSKEIEGWKPAFILKKNVEEDTLYELLETATDILDSLA
                     NMEVYQLSIIDKTIKDSIYNSFWTAVFEEFDKLLAHYEKYRRLRFDNNYSMPENIITA
                     ESIHILDEIISSGKETPVTLFTAVTKPKWKKLRDEITNNGRVIENRADFENVKLIIEY
                     EAQRAQILNKINKLLSEINEAVPLSVDSFEEKAKQYHKKILSSLCWYKKVWSEFTNKL
                     AELCSDKAKFEELCTIDLDCPVESITKLLKVVLLSDLKKRYVAELLSKLANEWTGFAV
                     YLEEYKTYGQPFEELYYAVVEKDANVYTKVYQLLSDILEKKEMYIRRCRLIEQLKIYA
                     PDWTKAIQNREGIHGEHQLPENIEAAWKWRQLSNQIARIDNYDVNAIQKELSYINEQL
                     MQNARKLAYEKAWYEKIKNKTGAQTQAIEGWRTTIKQVGKGTGKTAPALLKKARELMP
                     LCQTAIPVWIMPLNRVAENFDPQKNKFDVVIIDEASQADILALSALYLGKKVIIVGDD
                     EQVSPDTVGIKAEEISALIEQHLQGVPNSHLYNGKTSVYDMAKAAGFKPMMLTEHFRC
                     LPEIIEFSNQLSYNGKIKPLRDASTVNIKPSVVECRVPNAFKTPKKINEVEAEYIASL
                     ICACVADENYRGKTIGVISLLGQEQAYEIERLLQVNLDPKEYEKRRIQCGTASQFQGD
                     ERDIIFLSIVEGPSEKGGPVRLLSEDGNNDANRKKYNVAASRAKDQMWVVHSLNPEID
                     LKPDDIRLRLIKHAINPSIDKNEEKLKHAESDFENKVMNILLNKGYKVTPQWNVGAYR
                     IDMVVEDGDKRIALECDGEKWHTLEDLPKDLKRQAILERLGWRFIRIRGSAFYRNPKD
                     IMKWVFEELETYGIRPNYLSDEIDTSDQNSIGSKIIDGIKRHAEQIRREWHGEEVNTE
                     VNNRDRALNVIDINTYVKDRTERENITQMGTQEALYQKEEKQELKVINEDKFNIFGES
                     FKAEQLSFDLPDLNLTNRQLSIEDLNSLNFQRAINKEHTGLKEEQAETSLDEKAKNKP
                     DQELLTEQPNEKDICKLLKRKGYEFVDKRSQGGSLWVIGDEKLGKELKAFAALGFNFS
                     YTSKGGRATGHKPAWFSKNG"
     gene            complement(8933..9796)
                     /locus_tag="PK733_12850"
     CDS             complement(8933..9796)
                     /locus_tag="PK733_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11463.1"
                     /translation="MNRKKITELDKLFLEIGTYRKSSEFKELFEFVRRFPHIAPYNAM
                     LIHIQKPGSQYVASAAEWMRIFNRRVKTGARPLVILRPFGPVAFVFELGDTEGDEPFP
                     EDLINPFKVKGEVSDYYVKNLINNMKCEGISYLEADHGTSSAGFIKTCYEEKATKIIR
                     NKKEILIKVLFEMVVNRNHPKETQFSTILHELGHAYCGHICSADPKLWIEGRFIPKNE
                     CEFEAESVCWLICERMGIKNPSAEYLSGYLNSNNEIPNISIDAVLKATGIIETLIKEN
                     KEPRKDLILNK"
     gene            complement(9829..10866)
                     /locus_tag="PK733_12855"
     CDS             complement(9829..10866)
                     /locus_tag="PK733_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11464.1"
                     /translation="MRIDRLPKFVRDFAKKMKLPLDENVESYKFTAYKRCESEDVSLI
                     NQDHFLFKLGMGLTEAEFEKVALSRSMIKYPCKELLTVEIYNVGISDGTGRELTNQII
                     YFAKRSNGEAELLDPYWLYQCDFKGDIVLLEEKTDGDILSAVYSEIAKVKNKVKEKRE
                     KQLEKVSSFLSKTFQTQYNDTLEKLTQYQQNNIDNKNSALINQMNAALIDIEIRKEER
                     LSEVERQRNIIMKPPKKIGQLELVPDGMGCRVFPTDYKEIVEKYEHHNGRSNFKRFNS
                     YALVDFYSERYNGEPRFIIVTNMKNVLYTQDYLEDLQEIIDYTYIYFIDEDMSVTEYS
                     VKDYWMLYFRE"
BASE COUNT         3159 a         2288 c         1476 g         3987 t
ORIGIN      
        1 gataataccc gtaatacact actttggata tgatgttttt cttcttctgg atcttcatag
       61 tagttatacc gcctattatc tcctcgaagc tgctcaatag ctctaaaaac ttgggttagc
      121 ggacgaaaat agtgtgcaac ttctgcccac ttttttacgg cttgaatgtt tccatctttt
      181 attgtatcta caccttgtga gccatttaat aattcaacag gctctgcaac attgaaaata
      241 ttccttgctg attcatatgc ccctttttca attaacaaac tacaaaattc aagtgctttt
      301 gcctgcgaag ttcttaactc tttaccgtgt attatatgct tgattgctgc atctacgcca
      361 tttagttcca ataacaacct tgggaggtca acgtcattaa tattctcatc tctctccctg
      421 agttcctttt ctatgagaaa ttgtcgtatt acacataaac catcattctc ttttgcagca
      481 gctttaagac acaatgttat atcatctatg atgtcatcca atggccgaag tgaataatat
      541 tgttctctaa aataatcctg ttgtccaatc tcaatagtct tcttgtcttc tccggcacac
      601 gaataatgat acaatttttc ccatgaccat tcaacgtcgg catcattatt tgaagcaaag
      661 tgtgcaagct gtttatggaa gtgactattc tgatctctcc caagtaaatc tcgttctgtc
      721 ttttgcaata taaattgtct aaaactatta tgaaaaaagt gccaacgggt aaaattatcc
      781 actctaaaga agtgctttgt ctgtataata aaacgttcta caattgttag tccaaaccac
      841 ttttcgacct caattaagtc tattgttcct cgtaatctcg aaataagtgc taataattcc
      901 ctgatatctt tatcagatga tatacttttc caatacactt catatgcttg atcgatatgt
      961 ccagtataag gattagatga attaagaatt gtatctattt catcatgcat cctataagtt
     1021 tttagttttt gcaataagta atgaagagcc agtgggtgcc cgtctgagag tttaaatatc
     1081 gtatcctttt gaatatttgt taattcaata ggaaattcaa tagaatcgat gacattaaaa
     1141 acatcagtac gccctaatgg attcatcgtt aaggtacgtc cctcttgctg cagttgtgtt
     1201 tgtatatttg gtgataatcc agctaaatca atcttttgtg taccaagaat aaacaaagta
     1261 ccttctggta ttgcttctgg attaggtaga tccattaata atgttctttc cggtgattgt
     1321 tctctttcta tatgatctag tccatctact ataataattg tgcgaattcc atctacttgc
     1381 cacattgatc ttaattctga gagctgagaa cccacgactt ctaataattc ttctctactc
     1441 tctggaaatg ctttaccttt ccctcttata ccatgattct tcagtgctaa aactaagtcg
     1501 tgtagaaaat ttaaagattc acctcttcct aaacgcgtat catcttttat atatgcataa
     1561 tagcggataa ctctatagcc ttttctatat ctgaaggtct gagtaagtgt tgttgattta
     1621 ccagagccgg gcgtaccaat tagtgcaagg tagccattat taaaactatt gagactttgt
     1681 tcaatgtcat taatggttga taaaataggt tgataagtct tttcatccac ccagaattca
     1741 tgcggaaatc taaacttaaa tctattttcc catcctaaag attttaacag ttcctggcaa
     1801 gtaattttaa gaatcctttt ttgcccacca gcagcattca ttagaaattt aaatatttca
     1861 tttttatctt ttcttctttg tagtgttact gattcctgat cttctggttc aaaaatttgg
     1921 taattgaagt caaaataaca atcctttaca aagagtaaaa atttctcttc atcaaagcaa
     1981 gtaacatctt taattgtgtt tatagcagct ttccatccct tagggattac gctaaaacca
     2041 tcttttatcc aatttttatc atcccagcaa tcccttaaaa acgcttggaa atgtttatac
     2101 cgaggaggtt cgtcatctga aacaattgta tcgtttgtag aaggtatatc atttgatact
     2161 aaatgaacat ttataattct atcagtatga atcctggtca tagcctccca accttctgca
     2221 agctgcataa ttagtggagg tttgataact ttgtttttgc ttttctgcgg tttaataata
     2281 tcattaaatg tttttgctcc tgcaaattct ccccatttaa cttgatatgc atccaatctc
     2341 ccctgtgttg caatttgaat atcatccagc ttctcagctt cggggtccat aattccgatc
     2401 cattcaaaag taccttttaa taaagcgtca tatataagtt tagctgcaat cttgtactgg
     2461 ggtacaaatc ctatcagtgc actcctttct ccatcggcag cattatgtat gtgattattt
     2521 tctccatcct tcatttgtat tacctcactt tataaagctc agttaataat attaaataat
     2581 gagtacattt tagtttacta taattgccaa aaaggtactc aagcaagctt agtcataatc
     2641 tagcaaacag ctttttatat gcatgttgct acttgacatg ataattctct ctccagtgtt
     2701 aaactgtact tcaacgctct attgtgttcc ttaacaggaa gtaagcatca tatacatgat
     2761 aagtcataat aaaactacat gtcagattca ccttatatta tataatgaat tgtctgaacc
     2821 tcttttggac aaatcagctt tctttttggt gtaaatcttg cactatagtc attttttata
     2881 ttttatagtt atcatatatt tttataatat cttccgggct gtcaaactgc atacctccag
     2941 gctgagaatc aaataaagac tgaatatcaa tgtatgctcc cattgcattt aatgcaagta
     3001 tcattttccc ttttgcatca tcaagctcat tattatcaat actttttatt acttcattaa
     3061 ttatctcacg cacatctgct gcccatatag cataccttga gtatgttgcc gaagcattat
     3121 catccaactg attaaaagct acacgttcca gcctactata aaccatagca tcagagttac
     3181 cattgcaata tcgattatca ttaaaaactt tttccttacc aagcgaagaa taaaagttta
     3241 gaataatcct aacatagtct ataaagtttt cttccattct catgtacctc acccacataa
     3301 taataattct atatataaac ccagtctcta tttatctcct gcaccagcca ggctcttgct
     3361 cagtttttgc agcattatag catctttacc tttgagcact acttcaccac attttgggca
     3421 tacatatgaa ctaacatctt ctacagtcaa cttatattga ccccatccag tgtttatggg
     3481 tacagttttc ttttccattt ccgtattaca gatatagcaa atcttaatca taatgaacct
     3541 cctacttggc gttttaagag tattttatcc aaatcattaa tgattgtttg tttcatccat
     3601 tctttgaaaa ccaagcaggc ttatggcctg ttgctctccc tcctttggat gtatagctga
     3661 aattgaagcc taaagcagca aaagctttta attcctttcc aagcttttca tctccaataa
     3721 cccaaagaga acctccctgt gaccttttat ctacaaactc atatcctttc cttttcagga
     3781 gtttacaaat atccttctca ttaggctgtt ctgtcaacag ctcttgatct ggcttatttt
     3841 ttgctttttc atctaaggat gtctctgctt gctcctcttt taagcctgta tgttctttat
     3901 taatagccct ctgaaagttt aaactgttca aatcttcgat ggaaagctgt ctatttgtca
     3961 gatttaagtc tggcaaatca aaacttaact gctccgcttt aaagctctct ccaaagatat
     4021 taaatttatc ctcattgatt acctttaact cctgcttctc ttctttctga tataaagcct
     4081 cctgggtacc catttgcgta atattttccc tttctgttct atctttaacg tatgtattta
     4141 tatctattac atttaaagct ctgtctctat tattaacttc agtattaacc tcttcaccat
     4201 gccattccct acgaatctgt tcagcatgcc tttttatccc atcaattatc ttgcttccaa
     4261 tagaattctg atctgaggta tctatttcat cagataagta attgggcctt atgccgtaag
     4321 tctccaactc ttcaaaaacc catttcatta tatctttggg atttctataa aacgcacttc
     4381 ctcggattct aataaatctc caacctagcc tttccagaat ggcctgcctc tttaaatcct
     4441 tcggcagatc ctcaagtgta tgccactttt caccatcgca ttccaaggcg atacgcttat
     4501 ctccatcctc aacaaccata tctatcctat atgccccaac attccactga ggagttactt
     4561 tataaccttt gttaaggagt atgttcataa ctttattttc aaagtctgac tcggcatgtt
     4621 taagcttttc ttcattttta tcgatagatg gattaatggc atgcttaatc aacctaagtc
     4681 ttatatcatc aggcttcaga tctatttccg gatttagcga atgcacaacc cacatttgat
     4741 cttttgctct acttgctgca acattatact ttttcctatt agcatcatta ttgccatctt
     4801 cactcaatag ccttacaggg ccaccttttt cactaggtcc ttcaacaata ctcaagaata
     4861 ttatatctcg ctcatccccc tggaattggg aagcagttcc acattgaatc ctacgtttct
     4921 catattcttt agggtctaaa tttacttgta aaagcctttc tatttcatag gcttgctcct
     4981 gtcccagcag tgaaataacc cctattgttt tccctctgta attttcatct gcaacacaag
     5041 cacatatcag agaagcaata tactcagctt caacttcatt aattttttta ggtgtcttaa
     5101 aggcattggg aactctacat tcaaccacag agggttttat attaacagtt gatgcatccc
     5161 ttaaaggttt tatttttccg ttataagaca actgattact aaactcaata atctctggca
     5221 aacacctaaa atgctcagtt aacatcattg gcttaaagcc tgctgctttg gccatatcat
     5281 aaactgaggt ctttccatta tacaaatgac tatttggaac tccttgtaag tgttgttcaa
     5341 tcaatgcact tatttcttcg gcttttattc ccactgtatc agggcttact tgctcatcat
     5401 ctccaacaat aattactttt ttccccaaat ataatgctga aagagccagt atatctgctt
     5461 ggctggcttc atcaataatt actacatcaa atttattttt ctgcggatca aaattctcag
     5521 ctacacgatt taatggcatg atccatactg ggattgcagt ttggcaaagt ggcattaatt
     5581 ctcttgcttt cttcaataac gcaggtgctg tcttaccagt cccttttcca acctgcttta
     5641 ttgttgtacg ccacccctca attgcctgcg tttgtgcccc agttttattc ttaatctttt
     5701 cataccatgc cttttcataa gcaagctttc ttgcattctg cattaattgt tcattaatat
     5761 agctcaattc tttctgaata gcatttacat cataattatc aatccgggct atttgattgc
     5821 tcaattgacg ccatttccaa gctgcctcta tattttccgg caactgatgc tcaccatgga
     5881 taccttctct attctgaatt gctttagtcc aatccggagc ataaattttt agttgttcta
     5941 tcaatcggca acgccttata tacatttctt tcttttccag tatatcagaa agcaactgat
     6001 acacttttgt atatacattg gcgtcttttt caacaacagc atagtaaagc tcctcaaagg
     6061 gttgcccata agttttatat tcctccaaat aaaccgcaaa tccagtccat tcatttgcta
     6121 gtttacttaa taattctgca acatatctct ttttcaaatc agaaagcaat acaactttaa
     6181 gtaattttgt aatagattct acagggcaat caagatctat agtacatagt tcctcaaatt
     6241 tagctttatc tgaacataac tcagccaact tattagtaaa ttctgaccag accttcttat
     6301 accaacataa agaggaaaga atctttttat gatattgctt tgctttttcc tcaaagctat
     6361 ctaccgataa aggtacagct tcgttgattt ccgacaagag tttattaatc ttattaagta
     6421 tttgagccct ttgtgcttca tactcaataa taagctttac attttcaaaa tcagctctgt
     6481 tttcaataac ccttccgtta ttagttattt catccctaag ttttttccat ttaggtttag
     6541 taactgcagt aaataaagtc acaggtgttt cttttcctga gctgataatc tcatcaagaa
     6601 tatgtatact ttctgccgtt attatatttt ccggcatact atagttgtta tcaaagcgaa
     6661 gccttctata tttttcatag tgtgcaagta atttatcaaa ttcctcaaaa acagcagtcc
     6721 aaaaagagtt gtatatactg tccttaatag ttttatcaat aattgatagt tgataaactt
     6781 ccatgttagc taaactatcc agaatatcag tggcagtttc taaaagctca tataaagtgt
     6841 cttcttcaac atttttcttt aatataaaag ctggcttcca gccttcaatt tccttactat
     6901 atttaatgta gttttcagta ttaattctga aagagtcaat actccataat gaagctaaag
     6961 acggtaactt cttgcggagt agttcatctt cttccagtga tatttgctga tttgaatggt
     7021 acaaaaaatc caaattctct gtagacagtg gtaatccaat tctatcatca gaagttgacc
     7081 ctggaatata atcaaaatta ccgtatccag tattaataaa cttagctgca tctattggtg
     7141 atatcgtatg attatcaaat atgatatcct tatattccaa agatctgata tataaaagtt
     7201 cttggctttt agcctttgac ttacttatca gctctttccg ttcattctcc agtctttcaa
     7261 tttttttctt tgattcaaat aaatcagtag aggtgctttt tatggctatc tcatttatag
     7321 cgtcatccat ttctttcttt tgtgaattcg agcttaatag actaatacac aggctctgta
     7381 gatttatgtc tttattatat tgatctttat acaccttttc tttaagcaca gtcaaagcct
     7441 tttcagtatg gctggtaaca agcacactac acccttggct taacaaatgt cctattaaat
     7501 tcgctatagt atgtgttttt cctgttccag gaggtccttg taccagaact gcaccatagc
     7561 tattaagata tttgattatt ttgagctgct cattattagc tggtaaagtc agcagaacat
     7621 cttggtcaat accactttga ttccagttat cctcaataac atctatatca gtatgttctt
     7681 tataattacc gaccatattc ttaaaaaaag ccggtaaggt tatatcttta ttctctttaa
     7741 gttcttcaat gattttttca ataaacacag agaaccctaa agtcctcttc ctgagaaaca
     7801 gcgttggtgc agacataatc attggcccat tatagccatt ttcgaatact tctacacatt
     7861 tccctttttc atcaacgaca tttataagac gttgaaaaag tcccaggata tttgcagtat
     7921 ccgcaatatg gtatgcattt gcttcaatgt cttgaaggac atctgacaac attttctgat
     7981 taattgtagg aataatccta agcatgggtg tatagagttc tgttttcagt tcctcacatt
     8041 taataataaa agatggtttt tcagaatcaa attctaattg gactctttgt agtagtacag
     8101 gatgatctat tattctatca gccatactcc aacgaatatg accatcgccc aatataagct
     8161 ctacaccttc tgattccttc ttcatatcag aatataacct aaaaagatta ttgtataaag
     8221 tgagcccctg ttcttttggt atttctactg ctctccaatt attccttttc tttatccatt
     8281 catcaaacag gattttccga gtttcatcat caataaaaaa ctcctcaact tggtcaacct
     8341 tgtcatcttc attgagtgcc tcttttacta agacttcttt atactgaata ttttcaacat
     8401 taagcttttt ccagttaccc tcaagccaat ctaggatcga tttatctgga gcaggacatg
     8461 gctccaaagt gggcctttcg atttctaata tttttagctc attaaaatcc tgcacagtat
     8521 atatcgacca caactctttt atttgtggta agtctgaaaa tctcataatc catttttgtt
     8581 tatcaatctc tgttataaca ggattggaca gttcattgta atcttttaga aagctaaaaa
     8641 tgttatttat ttgagccatt tttacctcca gagtaacaat tacgtacaaa ataaattata
     8701 gtcacttttt accatttaca tgataactat aaagtttttt tgctaatccc tcattaactg
     8761 aataactaac tatacattat cacctctgta cttttacatt cctttttaag tcaatattcc
     8821 cgtccatgta ataaaatccc atattgctta ctacctaagt atctatctat tcaacaagaa
     8881 aatcaagtaa taccatacaa gttatacatt atatacaaat aaacaattca aactatttat
     8941 ttaaaattaa gtctttacga ggttctttgt tttcttttat tagagtctca ataattccgg
     9001 tagcctttaa aactgcatct atactaatat ttggaatttc attattacta ttaaggtatc
     9061 cactaaggta ctctgcagaa gggttcttaa tccccattct ttcgcaaatc agccaacaaa
     9121 cactttctgc ttcaaattca cattcatttt taggaatgaa acgcccttct atccacaatt
     9181 ttggatctgc agagcaaata tgcccacagt aagcatgtcc tagctcatga agaattgtag
     9241 aaaattgagt ttcttttgga tgatttctat tgacaaccat ttcaaacaaa acctttatca
     9301 gtatttcttt cttattacga ataattttag ttgctttttc ttcataacaa gtcttaataa
     9361 atcctgcact tgaagtacca tggtcagctt ctagatatga gattccttca cacttcatat
     9421 tattaataag attttttaca taataatctg aaacttcacc ctttactttg aacgggttaa
     9481 tcaaatcttc aggaaaaggt tcgtcacctt ctgtatcacc taactcaaaa acaaatgcta
     9541 caggtccgaa tgggcgaaga ataacaagag gtcttgcacc tgttttgact cttctattga
     9601 atattctcat ccattccgct gccgaagcaa catattgact tccgggcttt tgaatatgta
     9661 taagcattgc gttgtatgga gcaatatgtg gaaaccttct cacaaactca aacaactctt
     9721 taaattcact actttttcta taagtaccaa tctctaagaa aagcttatct aactctgtaa
     9781 tcttttttct attcatctag acccctccaa gcaaaataat ggatcttctt attctcgaaa
     9841 atatagcatc cagtaatcct ttaccgaata ttctgtcaca ctcatatctt catcaatgaa
     9901 atagatatac gtataatcta taatctcttg taaatcctcc aaataatcct gtgtgtataa
     9961 tacatttttc atattagtca cgataataaa tcgtggctca ccattgtacc gttcactgta
    10021 gaaatccact aaggcataag agttgaacct tttgaaattg cttcttccat tgtggtgctc
    10081 atacttttca acaatctcct tataatctgt aggaaatact ctgcagccca tcccatctgg
    10141 caccaattca agctgtccta ttttcttggg cggtttcata ataatatttc gttgtctttc
    10201 aacctcagat aatctttctt ctttacggat ttctatatcg ataagagcag cattcatttg
    10261 attgatcagt gcactgtttt tattatctat attattttgc tgatattgag ttaacttttc
    10321 cagggtgtca ttatattgtg tttggaaggt cttgcttaag aaactgctaa ccttctcaag
    10381 ctgcttctct ctcttttctt taaccttatt cttcacttta gctatttcac tatagaccgc
    10441 agatagtatg tctccatctg tcttttcctc cagtaaaact atatctcctt taaagtcaca
    10501 ttgataaagc cagtatgggt caagcagctc tgcttctcca ttgcttctct tcgcaaaata
    10561 aataatctgg ttcgtaagtt ctcttcctgt accgtcagaa ataccaacat tgtatatttc
    10621 aactgtaagc agttctttac aaggatattt aatcatagac ctactaagag ctaccttctc
    10681 aaattcagct tctgttaacc ccatacccaa cttaaataaa aagtgatcct ggtttattaa
    10741 ggaaacatcc tctgattcac atcgtttata agctgtgaac ttataggact caacgttttc
    10801 atcaagtggt agcttcatct tctttgcgaa gtcccttaca aatttaggta gtctatcgat
    10861 tctcataatt ttatcctcac tggtctcatt cgtagtgtta atctatttga
//
LOCUS       DAOSDD010000151        10858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2322_length_10858_cov_5.454781, whole genome shotgun sequence.
ACCESSION   DAOSDD010000151 DAOSDD010000000
VERSION     DAOSDD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_10858_cov_5.454781"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK733_12860"
     CDS             complement(<1..687)
                     /locus_tag="PK733_12860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11465.1"
                     /translation="MSDIKQLLLSEYMPQSELVLEEHHISKPRFDVIDVHTHFGLIGF
                     DGDYSNEYDTMKSVEELKNYGVKKVVNLDGMWGEELDKMLKKTEPCEDFFITFGTVDA
                     ARLDDKDFEAYVRNTLRELKEKGIKGLKFLKDVSLVLKDSKGKYISIDDQRLKVIWET
                     AAELGLPVLIHIADPVAFFKPIDRFNERYDELQHRPEWSFYKPGMFTFEQLMEMQENL
                     LKSNPDTTFII"
     gene            complement(771..3053)
                     /locus_tag="PK733_12865"
     CDS             complement(771..3053)
                     /locus_tag="PK733_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11466.1"
                     /translation="MIYGGIELNNVFNEHKTGFRRKDYLQEGYEYFNRKGIGYILPED
                     FCNNESTFSLVIKYIDDEISYASACYHRIGEISGKRIGDNPAFTTASACAGYTLVGTG
                     RIRHVVFILSNPAFGRGCHGADVMVFGIRNILSAKLVKGVCEEAIETARAERPSPEEC
                     KPLINLNRPLQLVSTAGIDSHVNDDIAASLKHSLENMREICPLIRVLGFNGVESYVKW
                     NFIEYEKGTFDYRYYDAIIEFAAQYGLGWFPLLIGGSGYALPEWYHDSEGFTGFACLE
                     HGESNDIPTIFNDHQIDPVSNYLYKFGRHYNGNENVLGVRLGPSGNYGESQYPATGNW
                     GYKGTWQHMHIGWWAGDKDASTKFREFLKKRYNNDITKLNALWKEEYNSFDELETFLP
                     SSAFVPRKRKDFTDWYVFEMTDWCEKWAIWTREYLKTCDIYQSAGGWGFIESGTDFTD
                     QTRSMIKVNGGIRATNEDESYVLNFCITRMLSSAARFYGVDFGTEPAAFCSARGFIGR
                     LYNIIVNNGAHFFTYHPNFVFNDQGINKWLKYASLLERRAHPVIEVAVLYPDTMTKLE
                     DATLRHLDGSAWFSQVITLRSQLDFDYCSEQMILDGALEGYKVLVFLSRFQDGELIEN
                     KPLVEIDQWIRSGGTAIYCSVHHKGVCSVEGDYTIFNRWMAGDTGKGRVIVLGVDREP
                     VSRSTTAIKQALLQLDILGANTRKMLTMEKSEHVYASVLESGDFALLNFSYTPERVSI
                     EGAPPINMEPVSIELINPNE"
     gene            complement(3068..4324)
                     /locus_tag="PK733_12870"
     CDS             complement(3068..4324)
                     /locus_tag="PK733_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11467.1"
                     /translation="MEKEYVFLNKVEKQLIEEMEQIVTIDCHEHLISEEHRLKANVDF
                     FTLFSHYTRHDLINEGMSVSEYNMLHNKNIPLETRWSIFEPHWNNIRWASYARSALLT
                     AKKFYGADDINASTYKQISENIKKANTPGLYKRILKDTCNIGISLLQNDYPYIEVDKD
                     FFVPVTHMTFFNDTSSWKGLSRPSFNPGAKVNTLDDYLDAAEEFIAKTKSKGAVGLKM
                     PVRETGEPTRHEAVRAFEKLRDGSVEELPPVNPIYDYVMDRIASLAKEYDLVIAVHTG
                     YWSDFRRLNPLHIIPLLIRHPKTRFDIFHLGYPWVRETLMLGKGFPNVWLNLCWTHII
                     SQKFATDALSEALELVPANKILAFGADYDGPLEKVYGHLIMAREDIARALSGKIDSGQ
                     LSMNQAVEIMKKWLHDNPRELYKLEL"
     gene            4613..5692
                     /locus_tag="PK733_12875"
     CDS             4613..5692
                     /locus_tag="PK733_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11468.1"
                     /translation="MNAENGNKLKYMEIYNHFKGLIVSKVLVVGDKIPTESEIGEAFN
                     VSRITVTKALNMLSNDSFIYRIPGSGTYVKEAEPSKDDNSLNLISLIASFKPQGREIE
                     LIQGIENYLKQNGFLLSVSNSNDDLDLEKELIYSVKDKVRGIILYPCLSTKNTDVFYE
                     LFKENYPVIYVDKYPFNVPCNYVVSDNFNGGYSIGKYFIEKNHKRMALIFHDLAAQTS
                     ELDRFNGFMKAVGEKGINRDDIKIISLGKNISSESKIELYRQLLKDKNTGDDLTAIFA
                     CNDVLAYDLMKHIAQENYNLPDNFILAGFDDLNTVSPNLPFITVHQELYDIGEAAAKL
                     ILKIVDNNMYVNEHQIIPVRLVEKL"
     gene            complement(5835..6542)
                     /locus_tag="PK733_12880"
     CDS             complement(5835..6542)
                     /locus_tag="PK733_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11469.1"
                     /translation="MAEKAQIIEANIIEWHNIMGLYASRVIVPHNGGPVDYSTATRFC
                     DVVHSVCWTSKHLSGVCFKYSYLKKSGAPEREIERVRLRADELFEAVYRCQLVTGVPG
                     LQARGYLLGHGETYGERVKGSRSRNAWHQGKGEYSNFRWRGDPSHHNYSDSAAGMGHY
                     YDRELQQDYLSQRIMAQVGKVPIYACNSIKMISSRLAEPLMLMHIGEQDIMVLLMIKL
                     LKYIINWREYINKIYCF"
     gene            complement(6785..8923)
                     /gene="bglX"
                     /locus_tag="PK733_12885"
     CDS             complement(6785..8923)
                     /gene="bglX"
                     /locus_tag="PK733_12885"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOJ11470.1"
                     /translation="MSIEEKLAQMTQLSGIFFTSGKDTEITGPMKEMGMSPEIINNAG
                     SILGTTGAQTIIDIQRSFLKNNPYGIPLLFMADVIHGFRTIFPIPLAMSCSWEPEIIE
                     ESAAISAREASVSGISVTFSPMVDLVRDARWGRVAESIGEDPYLASLFAKAFVRGYQG
                     GDLRNHTDRIAACVKHFAAYGAAEAGREYNTVEMGEPALRQNYLPAYYGALEEGCQMV
                     MSAFNTLNGVPVTCNKWLLKDILRNEWGFKGPVITDWGAVSELIPHGVAEDGMEAAKK
                     AIEAGIDIEMATTNFARYAESLIEAGELDEALIDQAVLRILELKDNLGLFENPYRGAD
                     PEKEKEVLLCSEHRNAARRIAAKSMVLLKNNGVLPLSRTVNKLAVIGPYTEPVNILGC
                     WRCLGKNEEAVPLSSGILSKLTSNRVAFAKGCGISEGDDKDIADAVAAAKNADVIVLA
                     LGEHYKMTGEAGSRAYITLPGRQNELADAIFSLGKPTVIVLFNGRPLEIRNLAEKASA
                     ILEVWFPGTEGGSAIADILFGDESPSGRLTMSFPYTVGQVPIYYNCLNTGRPKGDDSN
                     TARNTSRYLDIPNAPLYPFGYGLTYTTFEYSNANLNSNILVRGSKIVLTVTVTNTGER
                     AGEETVQLYLRDISAVISRPVLELKDYKKVCIEPGESQDVSFDINESMLEYYNTNKIK
                     MSEQGKFTAFVGANSRDLIALNFEYKYNAK"
     gene            complement(8913..9035)
                     /locus_tag="PK733_12890"
     CDS             complement(8913..9035)
                     /locus_tag="PK733_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11471.1"
                     /translation="MEREKDKRKEEQMSRNEDRRKEEQRGGFCELIGVSEKNEH"
     gene            complement(9310..10356)
                     /locus_tag="PK733_12895"
     CDS             complement(9310..10356)
                     /locus_tag="PK733_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ11472.1"
                     /translation="MGMKVFKALSMEKLEEITDKIKNIKVALIGDLCLDVYWIADMTK
                     SELSRETPHFPLPVISERITPGAGGNAATNIAALKPLSLKVLGVAGKDWRGEILLSAL
                     KNEGINTDGVVVSEKVITNAYCKPIRKGISPVEYEDPRIDFANYEFLVEDAEKELIRQ
                     LESVIDTVDVLCVSDQMVFGCVTPKVREKIIFYGLQGKKIVVDSRDRIGQYKGVFLKP
                     NEVEGYKAVYSEIPPKDISFDKYPNIARLLAEGQQSKVCMTLGAKGCLYADDTTIVHV
                     PCYPIDPPIDFCGAGDTFLSAFSCALATGAKPWEAASFANMAAEVTIKKIGTTGTASP
                     EEIRERHQEIISKM"
     gene            complement(10383..>10858)
                     /locus_tag="PK733_12900"
     CDS             complement(10383..>10858)
                     /locus_tag="PK733_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11473.1"
                     /translation="YKNNVPATYHIALGTDIIHQHPIVDFSAIGATSGVDFHIMCHSV
                     SRLDKGVFLNFGSGVIGPEVFLKALSIARNLGYPTFNITTANFDLIDLGDYSSKIGYD
                     DPHYYYRPRKNIVNRPTSVGGKGWHFCGDHKQTIPNLYKRLIEKLPETDKGICEE"
BASE COUNT         3259 a         2276 c         1756 g         3567 t
ORIGIN      
        1 aatgataaat gttgtatcag gattgctttt cagaaggttc tcctgcattt ccataagctg
       61 ttcaaaggta aacattcccg gtttgtaaaa tgaccattca ggcctatgct gaagctcatc
      121 ataacgctca ttgaaacgat caatgggttt aaaaaaggct accggatctg caatatgaat
      181 aagaacagga agtccaagct ccgctgcagt ttcccatatt actttaagcc gctgatcatc
      241 aatggaaata tatttaccct tactatcctt taatacaaga cttacatcct ttaagaattt
      301 caatccttta atgccctttt cttttaattc tcgcaaagta ttcctgacat atgcctcaaa
      361 atccttatca tcaagtctgg ctgcatcaac agttccaaag gtaataaaga aatcttcaca
      421 gggttcggtt ttttttagca ttttatctag ttcttctccc cacataccgt ccaggtttac
      481 tacctttttt acaccgtaat tctttaattc ctcaaccgat ttcatagtgt cgtattcatt
      541 gctataatca ccatcaaatc ctattagccc aaaatgagta tgtacatcaa ttacatcaaa
      601 ccttggtttg gaaatatggt gctcttctaa taccagttca ctttgcggca tgtattcgga
      661 aagtaataat tgcttaatat cagacattta ttttcctctt cctttcctgg ctcaattatt
      721 ttgaagtgaa aaattagaga ttaagttcag tgttctttca ctttacaaag ttactcattg
      781 ggatttataa gttctataga tacaggctcc atattaattg gaggagctcc ttctattgat
      841 accctctcag gtgtataaga gaagttaagc aatgcaaagt caccactttc aaggacactg
      901 gcataaacat gctcactttt ttccattgta agcatttttc tagtgttagc tcctaagata
      961 tccagttgga gcaaggcctg tttaatggca gtggtagagc gtgatactgg ttcacgatct
     1021 acacctaaaa ctataacccg gcctttgccc gtatcccctg ccatccatct attaaagatt
     1081 gtataatcac cttcaacaga gcaaactcct ttgtggtgta cagagcaata tatagcagtt
     1141 ccgcctgaac gaatccactg gtcaatttct acaagtggct tgttttctat caattctcca
     1201 tcctggaaac gagagagaaa aacaaggacc ttgtaacctt caagggcacc atccaatatc
     1261 atctgctcgc tgcaatagtc aaaatccagt tggctcctta aagttatgac ctgtgaaaac
     1321 caggctgatc catcaaggtg ccggagagta gcatcttcca gctttgtcat agtgtcaggg
     1381 taaagtacgg ctacctcgat aaccggatgt gccctgcgtt caagtaaaga tgcatatttg
     1441 agccatttat tgataccctg gtcattaaat acaaagtttg gatgataagt gaagaaatga
     1501 gctccattat taacaataat attataaagt cttccaataa aaccccgggc agaacagaaa
     1561 gcagctggtt cagtaccgaa atccactcca taaaagcgtg cagcactaga aagcattcgg
     1621 gtaatgcaga agtttagaac atagctttca tcttcgtttg tagctcttat gcctccattt
     1681 accttgatca ttgagcgggt ttgatcagta aaatcagttc ctgattctat aaatccccaa
     1741 ccaccggccg attgatatat atcacatgtt ttaaggtact ctcttgtcca gattgcccac
     1801 ttctcacacc agtcagtcat ttcaaataca taccaatcag taaaatcttt ccgtttacgg
     1861 ggaacaaatg cagaagatgg aagaaatgtt tccaattcgt caaaactatt atattcttcc
     1921 ttccataaag catttagttt tgttatgtca ttattgtatc tctttttcaa aaattcgcgg
     1981 aatttagttg aggcatcctt atctcctgcc caccagccga tatgcatatg ctgccatgtg
     2041 cctttataac cccagtttcc cgttgcagga tattgacttt caccataatt gccgctaggt
     2101 ccaaggcgta cccctaatac attttcatta ccattataat gcctgccaaa tttataaagg
     2161 tagttagata cagggtcaat ctgatgatca ttaaatattg tgggaatatc attggattcc
     2221 ccatgttcaa ggcaagcgaa accagtaaat ccctcactat catgatacca ttcaggtaat
     2281 gcataaccag agcccccaat gagcaaagga aaccatccaa gtccatattg tgctgcaaat
     2341 tctatgattg catcataata tctataatca aaggtacctt tttcatactc aataaaattc
     2401 catttcacat aactttcaac gccattgaaa cctaatactc gaataagtgg acagatttca
     2461 cgcatgtttt caagtgaatg cttcagtgaa gcagcaatat catcgttgac atgggaatca
     2521 ataccagctg tactaacaag ctgcaatgga cgatttagat ttatcaaagg cttgcattct
     2581 tcaggactgg gacgttcagc acgtgcagtt tctattgctt cttcacatac tccttttacc
     2641 aactttgctg acaagatgtt ccggattcca aaaaccataa catctgcacc atgacaaccc
     2701 cttccaaaag caggattgga taatataaat acaacatgcc ggattctgcc ggtaccaaca
     2761 agtgtataac cagcacatgc cgaagcagtt gtgaatgccg gattatcacc aatacgctta
     2821 ccggaaattt caccaattct gtggtagcaa gcagaagcat agcttatttc atcatctata
     2881 tatttaataa ccagtgaaaa agtactttca ttattacaaa aatcttcagg caaaatataa
     2941 ccgatgcctt tacggttaaa atattcgtaa ccttcctgga gatagtcttt tcttctgaaa
     3001 cctgttttat gttcattaaa aacgttatta agttctattc caccatatat cacgaattaa
     3061 gcccccttta taattcaagc ttatataatt ctctagggtt atcatgcaac catttcttca
     3121 taatttctac agcctgattc atagataatt gtccagaatc aattttccct gacaatgctc
     3181 ttgcaatgtc ttctcttgcc ataatcaaat gcccatacac cttttctaaa ggcccatcat
     3241 aatctgcacc aaaagccagg attttatttg ccgggaccag ttccagagcc tcgcttaatg
     3301 catcagtggc gaacttctgt gatattatat gtgtccagca aagattaagc catacatttg
     3361 gaaagccttt tccaagcatc aatgtctctc ttacccacgg ataccccaga tggaatatat
     3421 caaatcttgt ttttggatgt ctaattaaaa gaggtattat gtgcagtgga ttcaaccttc
     3481 taaaatcact ccaatatcct gtatgtacag caatcaccaa atcatattct ttcgccagcg
     3541 atgctatacg gtccatgaca tagtcataga ttggattgac cggagggagc tcttcaacac
     3601 taccatccct tagcttttca aaagctctca ctgcctcatg tcttgtaggt tctcctgttt
     3661 ctctcaccgg cattttcaaa cctacagcac cttttgattt ggtcttggct atgaattctt
     3721 cagcagcatc aagatagtca tccagcgtgt tcactttagc cccgggattg aaggaaggcc
     3781 tggataatcc cttccagctg cttgtgtcat taaaaaaagt catgtgagtt acaggtacaa
     3841 aaaagtcttt atccacttca atataaggat agtcattctg cagaagagaa attcctatat
     3901 tgcatgtgtc cttcaatatc cttttgtaca acccaggagt atttgctttc tttatgttct
     3961 cagaaatttg tttataggtt gaggcattta tgtcatcagc gccataaaac tttttagccg
     4021 ttaacaatgc cgaacgggca tagcttgccc atcgtatatt attccagtga ggttcaaata
     4081 tggaccagcg agtttcaaga ggtatatttt tattatgcag catattgtat tctgacacac
     4141 tcatgccttc attaattaaa tcatgccgtg tgtaatgaga gaaaagagta aagaaatcca
     4201 catttgcttt taatcggtgc tcttccgata tcaagtgttc atggcagtca atagtaacta
     4261 tctgttccat ttcttcaata agttgctttt cgactttgtt taaaaaaaca tattcttttt
     4321 ccatagaatc ccccctaaaa agtaagagga cacgtcatcc cgagtaagaa ccacatccaa
     4381 attcatcctc tttatttgac atgcttaatt atattaatta tatcatttaa aattcaaaaa
     4441 agcaacttgt tttcataaat aaaaataaca acttgagttg aaattaatat agcaaaattt
     4501 atctcttaga aatgttgtta tattttttcc atttgacata tcaaatatct tgctatataa
     4561 tatttttaga ttaacaataa taataattta ctaattatga aggtgatctt atatgaatgc
     4621 agaaaatggc aacaaactaa agtacatgga aatatataat cattttaagg gattaatagt
     4681 atccaaagtt ctggttgtcg gagataaaat tcctacagag agcgaaattg gtgaagcttt
     4741 taatgttagc agaataaccg taacaaaagc tttaaatatg ctgtctaatg acagttttat
     4801 atatagaatt cccggaagtg gaacatatgt aaaagaagca gaaccatcaa aggacgataa
     4861 cagtcttaat ttaatatctt taattgcttc tttcaaacca cagggacgtg agattgagtt
     4921 aatacaaggt atagagaatt accttaagca aaatggtttt ttattgtcag taagcaattc
     4981 aaatgatgat cttgatttag aaaaagaatt aatatatagt gttaaagaca aggtgagagg
     5041 aataatacta tatccttgtt taagtactaa aaatactgat gttttttatg agttgtttaa
     5101 agagaattac cctgttatat atgttgataa atatccattt aatgttccct gtaattatgt
     5161 agtatcagac aactttaatg gcggatacag tattggcaag tattttattg aaaaaaatca
     5221 taaacgaatg gcattaatat ttcatgacct ggcagctcaa acttctgaat tggacaggtt
     5281 taacggtttt atgaaggcag ttggtgaaaa aggaattaac agagatgata taaaaattat
     5341 atctttaggt aaaaacatat cttccgaaag taaaattgaa ttgtatcgcc aattactgaa
     5401 agataaaaat actggagatg atttgaccgc aatttttgca tgtaatgatg ttttagcata
     5461 tgatctcatg aaacatattg ctcaagagaa ttataatctg cctgataatt ttattttagc
     5521 cggattcgat gacctgaata cagtttctcc taatttgcca tttatcacag tacatcagga
     5581 gctttatgat attggagaag ctgcagccaa attgatatta aaaattgtag ataacaatat
     5641 gtatgtaaac gaacaccaga ttattcctgt caggctggta gaaaagcttt gaattggtca
     5701 aataagagag tgcagcaatg cactctctta tttaaattac tctttaatta aataattcct
     5761 ccgtatttaa aatcctttga ttcgtattcg tcctttcttc ttttgataaa tgactatgag
     5821 catcaattaa taactcagaa gcaatatatt ttatttatgt attccctcca attaataatg
     5881 tatttcagca gttttatcat caataaaacc ataatatctt gctctccaat atgcatcaac
     5941 ataagaggct ccgccaatct gctgcttatc atttttatag aattgcaggc ataaattggc
     6001 actttcccaa cttgcgccat tatcctttga gacaaataat cctgctggag ttcccgatca
     6061 taataatggc ccattccagc tgcggaatca ctgtaattat gatggcttgg atcgcccctc
     6121 cagcggaaat tactatattc cccctttccc tgatgccagg catttcttga tctgctcccc
     6181 tttactcttt cgccgtaagt ttccccatga cccaataaat atcctcgtgc ttgaagtccg
     6241 ggaacacccg tcaccaattg gcatctatat accgcttcaa ataattcatc agctcttaat
     6301 cttactcttt caatttctct ttcaggagca cctgattttt taagatagct atatttgaaa
     6361 catactcccg acaagtgctt tgaagtccaa caaacagaat ggacaacatc acaaaatctt
     6421 gtagctgttg aatagtctac gggacctcca ttgtgaggaa caatgactct agatgcatat
     6481 aagcccatta tattatgcca ttcaataata tttgcttcaa taatttgagc cttttctgcc
     6541 aatgaaagct tctaggattc attattatgg aaatgtttat tcttaatcat attcttattc
     6601 cacttactcc ctttcaaata tttttttcga atatttttat agcgaaaact tgattctact
     6661 tcaaaaaaat catgttccaa caaaattttg atagcatggg aaagatgctt tgtttccaca
     6721 gtagactttt gcagtggaat agagttgttg cagcagaatc aaacttgaat ttattccttg
     6781 taaattattt tgcgttatat ttatattcaa aattaagagc aattaaatct cttgagttgg
     6841 cacccacaaa agctgtaaac ttaccctgct ccgacatttt tattttattt gtattataat
     6901 attccagcat gctttcatta atgtcaaatg acacatcctg tgactcacca ggttcaatac
     6961 atactttctt gtagtccttc aattctagaa caggacgtga aatgactgca ctaatatctc
     7021 ttagatagag ctgaactgtt tcttctccgg ctctctctcc ggtgttagta acagttacag
     7081 tcaacactat tttgctgcct cgaacaagta tattagaatt tagatttgca ttggaatact
     7141 caaaagttgt atatgtaagt ccataaccga aaggataaag cggagcatta ggtatgtcaa
     7201 gatatctgga ggtattgcgt gctgtgttgc tatcatcacc ttttgggcgg cctgtattca
     7261 agcaattgta atatatgggt acctgtccta cagtatatgg aaaactcata gtgagacgtc
     7321 ctgacgggct ttcatctcca aagagaatat ctgcaattgc actaccacct tctgttccag
     7381 ggaaccagac ttcaagtatt gcagatgctt tttctgccag attgcggatt tctaggggac
     7441 gtccattaaa aagtacaata acagtaggtt ttccaagact gaatattgca tcagccagtt
     7501 cattctgtcg tccgggtaaa gttatatacg cacgacttcc ggcttcacca gtcatcttat
     7561 agtgttctcc caaagcaaga actattacat cagcgttttt tgcagcagct acagcgtctg
     7621 cgatatcttt atcatcccct tcacttatcc cgcagccttt tgcgaaagcg actctattac
     7681 tggttagttt ggaaagtatg ccgctggaaa gaggcaccgc ttcttcattt ttacccaggc
     7741 aacgccagca gcccagtata ttaacaggtt cagtatatgg gccaataact gctaatttgt
     7801 tcactgttct ggaaagcggc aaaacaccat tatttttcag caataccata gattttgcag
     7861 caatacgccg ggcagcattt ctatgctcac tgcataataa tacttccttt tctttttccg
     7921 ggtctgctcc tctatatgga ttctcaaaaa gacccaggtt gtcttttagc tcaagtatac
     7981 gcagaacagc ttggtcaatt aaagcctcat ccagttctcc cgcttctata agagactctg
     8041 catacctggc aaaattcgtt gtggccattt ctatatcaat accggcttca atagcctttt
     8101 ttgcagcttc catgccatct tctgcaacac catgggggat taactcgctg acagcacccc
     8161 agtctgtaat aaccggacct ttgaagcccc attcgtttct gagaatatcc tttaaaagcc
     8221 atttattgca ggttaccggc actccattta gtgtattaaa agctgacata accatttgac
     8281 agccctcttc caatgctccg tagtatgccg gaagataatt ttgccttaaa gcaggttctc
     8341 ccatttcaac tgtattatac tcccttccgg cttctgcagc accatatgca gcaaaatgtt
     8401 ttacacatgc cgcaattcgg tctgtatgat tccttaagtc gcctccctgg tatcccctta
     8461 cgaaagcttt tgcaaacaac gaagcaagat atgggtcttc acctattgat tctgcaactc
     8521 ttccccatcg ggcatccctt acaaggtcaa ccatgggaga aaaggttact gaaatacctg
     8581 aaacagaagc ttctcttgcc gatatagctg ctgattcttc tataatttca ggttcccagg
     8641 agcaactcat ggctaaagga attgggaaga ttgtcctaaa gccgtgaata acatcagcca
     8701 taaataaaag aggaattcca taaggattgt ttttgagaaa actacgttgt atatcaatta
     8761 tagtttgtgc acctgttgta ccaagaatag agccggcatt attaatgatt tcagggctca
     8821 tccccatttc tttcattggt cctgttattt ctgtatcttt acctgatgta aaaaatatac
     8881 ccgaaagttg tgtcatttga gcaagctttt cttcaatgct catttttttc actaactcca
     8941 ataagttcac aaaaccctcc cctttgctct tcttttcgtc tatcctcatt ccttgacatt
     9001 tgctcttctt ttcttttatc cttctccctt tccatatata agaatctatt cgaaaacttt
     9061 taaagctttc gaatgctata tttttttaca atgttcacat attcccatat ataagttata
     9121 attagttaaa tttgcacttt tacttatctg tataagttct tccaattagt tttcgaattc
     9181 atacataaag tcaaattacg aatagacttt cttccaatac tttatgttat gtattctaga
     9241 ttaattttga tgttatcaat ttattttaaa catataattt tttagattac aaccaataat
     9301 cgaaattcat tacatttttg aaataatctc ctgatgtctt tctctaattt cttcaggact
     9361 ggcagtgccg gtagttccga tcttttttat ggtcacttca gctgccatgt tggcgaacga
     9421 tgctgcctcc cagggtttcg cacctgttgc aagtgcacag gaaaatgctg aaaggaaggt
     9481 atcaccagcg ccacaaaagt caatcggagg atctattggg tagcaaggta catgaacaat
     9541 tgtggtatca tcggcatata aacatccttt tgctcccaaa gtcatacata cttttgattg
     9601 ctgcccttct gcaagaagcc ttgctatgtt tggatactta tcaaaggaaa tatctttagg
     9661 tggaatttca ctatagaccg ctttatatcc ttctacctca ttgggtttca aaaagactcc
     9721 tttatactgt ccgatcctgt cgcgactatc tactacgatc ttctttccct gaagtccata
     9781 aaaaataatt ttctccctga cttttggcgt tacacatcca aataccatct gatctgacac
     9841 gcaaagaaca tctactgtgt ctattacact ttccaattga cgaatcaatt ctttttctgc
     9901 atcttctact aaaaattcat agtttgcaaa atcaattctc ggatcctcat actctacagg
     9961 tgatatgcct tttcttattg gtttgcagta agcatttgta ataacttttt cactaactac
    10021 tactccatcc gtattaatac cttcgttttt caaggcacta agaagtattt ctcctcgcca
    10081 atccttgcct gctacaccaa gcacctttaa actcaaaggt tttagagcag caatgttcgt
    10141 agcagcattt ccacctgccc ccggtgttat acgttcactt atcactggta aaggaaagtg
    10201 gggagtttcc cttgacagtt cacttttagt catatcagct atccagtata catccaagca
    10261 aagatctcct attaaagcta cttttatatt tttaatttta tcagtaattt cctcaagttt
    10321 ttccatactt agggctttga atactttcat tcccatgttt tatataattc ctttcctaat
    10381 aattattctt cacaaatacc tttatccgtt tccggtaatt tttcaataag tctcttatat
    10441 aaatttggta tggtttgttt gtgatctcca cagaaatgcc aacccttccc tcctacgcta
    10501 gttggcctgt ttactatatt cttacgaggg cgataataat agtggggatc atcataccct
    10561 attttggagc tatagtctcc aagatcaatt aagtcaaaat ttgctgtagt gatattaaaa
    10621 gttggatatc ccaaattcct tgctatggat aaagctttta aaaatacttc aggtccgata
    10681 accccggagc caaaatttaa gaaaacacct ttatctagtc gggatactga atgacacatt
    10741 atatgaaaat caacaccgct ggttgctcca atagcagaga aatcgacaat tgggtgttgg
    10801 tgaattatat ctgtacctaa tgcaatatga taggttgcag gaacattgtt cttatacg
//
LOCUS       DAOSDD010000152        10814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2336_length_10814_cov_5.510363, whole genome shotgun sequence.
ACCESSION   DAOSDD010000152 DAOSDD010000000
VERSION     DAOSDD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_10814_cov_5.510363"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1175)
                     /locus_tag="PK733_12905"
     CDS             complement(309..1175)
                     /locus_tag="PK733_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11474.1"
                     /translation="MKIKGISIWTVFKVAFLAFMVLITIYPFIYMTSVSLSDKLSVMR
                     NEIVLFPKGVNIESYKMVLREERIAKAYLNTIIYVVLGTAISMILTTIGAYGLSKSKR
                     LIGFKFFNIVIVVTMFFGGGMIPKYLVVKSLGLLDTIWAVVLPSSISAWNLIVMRTFF
                     INFPEEIEQSGWLDGLNDFGILFYLVIPTSGAILATMSLFHSVGYWNSFMEPFLYLKT
                     ESKYPLQVILRSIIIQGSSLDNEYASGDSAVYEDSLKFATIIVSVIPIICIYPFLQKY
                     FVKGVMIGSLKG"
     gene            complement(1322..2296)
                     /locus_tag="PK733_12910"
     CDS             complement(1322..2296)
                     /locus_tag="PK733_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11475.1"
                     /translation="MKSMKSMKSMKSMKEKTLTCPAKKNNFLKKLNDSKYLLLMVIPV
                     MIYYFLFSYLPMYGITIAFKQYSPFKGYIASPWVGFKHFKTFSEYPFAWRLIKNTFLL
                     SLYSLLWGFPAPIIFALILNEVKNERGKKFVQTITYMPHFISTVVIVSMLTMFLSPST
                     GVFNKFIVSLGFEKVNFMSDARYFRSIYIASGIWSGVGWGAIIYLAALSNIDPTLYES
                     AMIDGANKLQKIIHINIPCIAPTIITLLILRTGNLMSVGFEKAFLLQGTINFEVSDVI
                     QTYVYRSGIKGGQMSYATAIGLFNSIVNILFLLSANYLSRRVNETSLW"
     gene            complement(2360..3910)
                     /locus_tag="PK733_12915"
     CDS             complement(2360..3910)
                     /locus_tag="PK733_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11476.1"
                     /translation="MKRFLALMLMVVMIVTLVSCVQKGKPSDSSNTTTADLATDKAHS
                     TLLFDEPVKITIMAPYHDSWPFQEDWYVLDLIKRKTNVTMVMDAVASTGWVDKYNITM
                     ASTDLPDIIWDFTAAQANTYGPEGAFVNILDHMDKLPNFKKFYEQYKPNVISNMSSDG
                     ALYSFPIYGIGNTNLRGWLYRKDIFDKHNIPVPANGDEFYNVLKQLKGLYPESYPLTF
                     RENLTHFKMAATAWGTCNTEYYDWDKKVWKFGPTEENFKDMLAYYKKLYQEELIPMDW
                     HTLTVQQWTQIVTTGNAFLTLDYIARVDTFGNPMRNENPEVDFRYMVPPAFGKNGQQK
                     LRFSCESNTVMSPCAGKNQDNTLKFLDWMYSDEGKDILSWGEEGVTYEIVNGERKWKP
                     IEGMEISAATLRQKYGLSTNGMYLFFDYNSHAANFSLQLKEADTLAQKYQDIQIPMVS
                     LNQDEDAIDKTKGVAIRDHYRANAAKFILGERPLEEFEAYVQELKALGLEEYLDILGK
                     AFKRQTGS"
     gene            complement(4228..6462)
                     /locus_tag="PK733_12920"
     CDS             complement(4228..6462)
                     /locus_tag="PK733_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11477.1"
                     /translation="MFKKIKGYLSINALFIRLFSSFLLIIIFISSSHVFTNSIYKKSI
                     ESEITENVDSRLQILVNELESKFYKVKNDIFLKTFSKFYQDLRGSDLTPYKYGLIEGE
                     LNDFKLTEKYISEIFIIIKTPMQLLSPNDMQFNTFFNNSTYLKDFWVQEIDEGFSFKF
                     YPAGYFADLSTSAAQTDNYLMPIAFKQSNNSKFIMVALINVKVLLESARSGFTEDLYV
                     FTKDGVLLFPESTGQVMFNERLLNGSGISAYTKMDNGYIFVLEGGLNELEYYKFYSNI
                     LVKQQINKSLVMIQFIIVCSILASGFIVLYIVKRFNNPVKQIVQLIRMQESNQEYNIS
                     SDFKSIAGNIKNLIEKNNTYEKRISRKDSLLKTFSCQVKMFDIFMGNSHAMDELSNIN
                     NINDYAVIYLKAHYKPTFYDHIFESEGKGTFFLKELIENYINNCFGSSITFQVGGDQI
                     ISIVNAKDDAENIHMHINEILNRLKAEEDYVFFTVASTEKYRNISDLKNAYNNAMEIA
                     EHRKLSGTSQFITEKSISNDPNIYYFSKEQQDQFMNSVQNGMIQESTRLITEILDFNY
                     KKEVTSFYMKLLCNKIVSCCSTVFMELYNRIPKRIKLADTYSSFNQCATIEDYKVSCL
                     NFLSVCINYINAYKKESDYIVDYIKNYVDKNYDKEIYLDLLSENLNMSKTYLSSYFKE
                     KTGINFIDYLNSYRIKKAIKLLEESPAKIKDIALKVGIPNLRTFNRSFKKYAGKSPNE
                     YRHS"
     gene            complement(6619..7692)
                     /locus_tag="PK733_12925"
     CDS             complement(6619..7692)
                     /locus_tag="PK733_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ11478.1"
                     /translation="MELRKDFTYSMVVPTSMGVRITPVNGQPVHSSDTFVMQATSAET
                     NVASISSYLGLTVKVLTKFVKDSPIARFIKDNLAGRHMSYEGLDVPQGGPWGYRHQFN
                     IADSGYGSRGPRVHNDRAGEVGRTLDIKDFDIERIFVKEGVQILHLSGLIAALSHETS
                     IFCLELAKFAKKNGTRISFDLNYRASFWKNREEELRNIFTEIASISDVLIGNEEDFQL
                     CLGIKGPEAGGKDIVSKIDSFKNMIKRVKEAYPNTSVFANTLRQVISTNSHMWGAIML
                     EGENWHVVEPREITVLDRIGGGDGFVGGLLYGILKGWEPEKWIQFGWATGAMATTFLT
                     DYAQPADEEQVWSIFEGNARVKR"
     gene            complement(8023..10233)
                     /locus_tag="PK733_12930"
     CDS             complement(8023..10233)
                     /locus_tag="PK733_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOJ11479.1"
                     /translation="MSIKNYISPIPQHFESLEGDEVVLGKPGKAMYQIEATSLPDDRL
                     AETAVELLCSYMTGLLNADASDAKGAIPIRLAVGKAPDNVKNPEQGYLLEVTPEAVTI
                     TGFGALGLYYGVITLKQCLKLEENILKLPCMRILDWPDLKTRGHFMESRYGSNLMTLD
                     DWKHVVDHMANMKMNQLVVSVYGCWCVQYDGRVSEYLYVPIKKYPELQTPVVARYFSP
                     TYNKWVDNEKLPPMFEQDFFGELIVYGKTRGVAVFPLFNSYGHNTLIPATYPEVSAKD
                     ENGEATLTGFCTSNPKTYELMFSIYDEIIDRYLIPNGIDSFHVGLDEVWDGLAHNAQD
                     IYKKRSPWCKCTECRNASRKHLFIDHAIKLLKHLKSRGIKNIYMYHDMLIGHGEADTA
                     ESAEDMVKALRDNDLIDNVVIDWWTYSDFQDKLMFQTTRPDLGFRCTVKPWNGYYHWT
                     VLTNPLRNIYLLAKMGTEEGVEGMQSYSAWDESYDRNHICQADYSWNYKGTGSIECMT
                     DRYVKRNFGAQYDKAQRAFELIDLITEVRNEKKEDGTPVLSKYGILLSTMSYYFYSYV
                     REGKPYPRIFPGEALRIINADRKNYLREINEIAVMAKEASALLDEVSADPRCNTKLAK
                     RFGYEVENYRCLAEDYLAIFKMIDIADTCCKCKYEKIKDMALQRKLARLALMTRLEQT
                     KEHFLMASHMRNHSIFMQFFSDLEGYMASTDPSEIKLDFFDMRYLESEAFRKLR"
BASE COUNT         3309 a         2138 c         1746 g         3621 t
ORIGIN      
        1 catccatatt gtataagatg tggggatgca ttttttctat tatacaaaat atggcagcat
       61 gcgattttcc gagtataaaa tttgaacaaa gcttacggat ttttctgaag acgtaaattc
      121 tgcaatccgt tgctgaaatg aatattatac atggaaatcg ttgctcacca tattttttat
      181 ttttcttcaa atataaaata tgcactctat gtacccgatt tctcttgacg gaacaagagg
      241 tttgctatac ttagaacata gtcgtatcaa gccttttaaa catttctcta cacaacattg
      301 tgtcgcacct atcctttaag ggagccgatc attacgccct ttacaaaata cttctgtaaa
      361 aacggatata tacaaataat aggtataaca gatacaataa ttgttgcaaa tttcaagcta
      421 tcctcatata ccgcagaatc gccgctagca tattcattat caagagaact gccctgaatt
      481 ataatacttc gcagaataac ctgcaaagga tatttcgatt cagttttcaa gtatagaaat
      541 ggttccataa atgaattcca gtaccccact gagtgaaata agctcatggt cgcaagtata
      601 gctccggatg taggaattac aagatagaaa agtataccaa agtcattaag accgtcaagc
      661 catccggatt gttcaatttc ctcaggaaag ttaataaaaa acgttctcat gacaataagg
      721 ttccatgcac ttattgagga aggcagcact acagcccaga tagtatcgag aagcccgagg
      781 ctttttacca caagatattt agggatcata cctcccccaa aaaacatagt aacaacaatt
      841 acaatattaa aaaacttaaa acctataagt cttttgcttt tacttaatcc ataagctcca
      901 atagttgtaa gaatcatgga tatagctgtt ccgagtacta cataaataat tgtatttaag
      961 tacgcttttg ctattctttc ttctctcaaa accattttat acgattcaat attaacaccc
     1021 tttgggaaaa gtactatttc attacgcata acactaagtt tgtccgaaag tgataccgag
     1081 gtcatataaa tgaatggata tatagtaatc aataccatga atgctaaaaa tgctacttta
     1141 aaaactgtcc atatagagat tcccttgatt ttcaattgat tccccctcct gttgctataa
     1201 acttgtttta ttaactaaat gctacctcaa atgcccaata ttattaaaaa cttctgttgc
     1261 tataaacttg ttttattaac taaatgctac cccaaatacg caatattgtt aaaaactctt
     1321 gttaccatag acttgtttca ttgactcgtc tggataaata gttggcagat aataggaaca
     1381 gaatatttac tatagaatta aaaagcccta ttgctgttgc ataactcatt tgtcctcctt
     1441 taataccgct cctgtaaaca tatgtttgaa taacatccga cacttcaaaa tttatcgttc
     1501 cttgcaataa gaacgctttt tcaaaaccaa ccgacataag gttgcctgtt cttagaatta
     1561 gcaatgtaat aattgtaggt gcgatacatg gaatatttat atgaattatc ttctgcagtt
     1621 tgtttgctcc atctatcatt gcagattcgt atagcgtagg atctatattt gataatgcag
     1681 caagatatat tatggccccc cagccaaccc cggaccatat acctgaggct atataaatgc
     1741 ttctgaaata tcttgcatca gacatgaaat tgaccttctc aaaacctaaa gaaactataa
     1801 atttattaaa tactcctgta cttggcgata aaaacatggt taacatactt acaataacta
     1861 ctgttgaaat aaaatggggc atataagtta tagtctgaac aaactttttt ccacgttcat
     1921 tttttacttc attcagaatg agggcaaata ttataggagc cgggaatccc cataaaagtg
     1981 aatataaaga cagcaggaat gtatttttta ttaatctcca cgcaaacgga tattcagaaa
     2041 acgttttaaa gtgtttaaac ccaacccacg gactggctat ataaccttta aaaggagaat
     2101 actgtttgaa agctatggta ataccgtaca taggcagata actaaataga aaatagtata
     2161 tcattactgg tataaccatt aaaagcagat atttactatc gttaagtttt tttaaaaagt
     2221 tgtttttttt cgcaggacat gttaaagtct tttctttcat tgatttcatt gatttcattg
     2281 atttcattga tttcattgaa gtctcacctc cgttggccgc caggcacatg atatgtgcct
     2341 ggcatgctta aagctaatat taagatccag tctgtctttt gaatgccttt ccaagtatat
     2401 caaggtattc ttcaagtccg agagccttga gctcctgaac atatgcttca aactcttcaa
     2461 gaggtctttc cccgagaatg aatttggcgg cattggctct gtaatggtct ctaatggcaa
     2521 ctcctttggt cttatcaatt gcatcttcgt cctgattcag agaaaccatt gggatctgaa
     2581 tatcctggta tttctgtgcc agtgtatctg cttctttcag ctgaagacta aagttcgcag
     2641 catgtgagtt atagtcaaag aagagataca taccatttgt agatagtcca tatttttggc
     2701 gcaaggtagc tgccgatatc tccatgcctt ctataggttt ccattttcgt tcaccgttaa
     2761 caatctcata agtaactcct tcttctcccc aggacagaat atcctttcct tcatctgaat
     2821 acatccagtc cagaaacttt aaggtattat cctggttttt tcccgcacaa ggagacataa
     2881 cagtattgga ttcgcaggaa aatcttaatt tttgctgtcc atttttaccg aaagctggcg
     2941 gcaccatata acgaaaatct acttcaggat tttcattcct catcgggttg ccaaaagtat
     3001 ctacccgtgc tatataatcc aatgttaaaa atgcgttacc tgtagtaact atttgtgtcc
     3061 actgttgaac tgtaagagta tgccaatcca taggaataag ttcttcctga tacagttttt
     3121 tgtaatacgc cagcatatct ttaaagtttt cttcagtagg tccgaacttc catacctttt
     3181 tgtcccaatc ataatactct gtattgcatg ttccccaggc agttgctgcc attttaaaat
     3241 gtgtaaggtt ttctctgaag gtcaggggat agctttccgg gtaaagtccc tttagttgtt
     3301 tcaagacatt ataaaactca tctccgtttg ctggaaccgg aatattatgc ttatcaaata
     3361 tatcttttct atatagccat ccccttagat ttgtattacc tataccataa atcgggaagc
     3421 tataaagagc tccatctgac gacatattgc ttattacatt gggtttatat tgttcataaa
     3481 actttttaaa gtttggcaat ttatccatgt gatccaatat attcacaaac gcaccttctg
     3541 gaccatatgt attagcctga gcagcagtga aatcccatat gatgtcagga agatcagttg
     3601 aagccatagt aatgttgtac ttatcaaccc aacctgtaga agcaaccgca tccattacca
     3661 tcgtgacatt cgtcttccgt tttatcagat ccagtacata ccaatcttcc tgaaagggcc
     3721 agctatcatg ataaggtgcc attattgtaa ttttaacagg ctcatcaaac aataaagttg
     3781 aatgtgcttt atccgttgcc aaatcagcag ttgtggtatt ggaactatcc gacggtttac
     3841 ctttttggac acatgacaca agtgttacta tcataactac cattaacatt aatgccagaa
     3901 atcttttcat attatattaa cctccattcc gccgctgccg ccagcagcat aaatagcaat
     3961 gaaatttatt ttcaagctac tttcctccta taataattaa atgtaccggc actctgcaga
     4021 gtatctattt ttattatatg tatgactatt tcaggaaaca atagtcattg ctaatgaaag
     4081 tggtcatttg ataaggcagc atgagtggca gcatgagcaa tatttagcag gtttcatcag
     4141 gccttttgct gtggtaccaa tagtttacgg taatcgaagg tagacatatt ttcagtttgt
     4201 agacagattt tcagtttatg gacattttta actgtgcctg tattcatttg gagatttgcc
     4261 tgcatatttt ttaaacgacc tgttaaatgt tcgcaagttt ggtataccaa ccttcagggc
     4321 aatatcctta atctttgccg gtgactcctc caagagcttt atggcctttt ttattcgata
     4381 ggaattaagg taatcaataa aattaatacc ggttttttct ttaaaatagg aagaaagata
     4441 cgtttttgac atatttaagt tttccgaaag taaatccaaa taaatctcct tatcataatt
     4501 tttatcaaca taattcttta tataatctac tatataatca ctctcttttt tatatgcatt
     4561 tatatagtta atgcaaacag ataagaaatt aaggcatgat accttataat cttcaatagt
     4621 agcacattga ttaaaagaag aatatgtatc agccagctta attcttttag gtatacggtt
     4681 ataaagctcc atgaagactg tactgcagca gcttactatc ttattgcata gaagtttcat
     4741 atagaagctg gtaacttctt tcttataatt aaagtcaaga atttcagtga taagcctggt
     4801 tgattcctgt atcattccat tctgaaccga attcataaat tgatcctgct gctctttgga
     4861 aaagtagtaa atattggggt cattactaat agacttctct gtaataaatt ggcttgtacc
     4921 agatagtttt ctatgttctg ctatttccat ggcattattg taagcatttt tcaagtcgga
     4981 aatattacga tatttttcag tacttgctac agtaaaaaag acataatctt cctcggcttt
     5041 taatctgttt agaatttcat ttatgtgcat atgaatgttt tcggcatcat ctttagcatt
     5101 aacaatagat ataatctgat ctccgcccac ctggaatgta atagaacttc caaagcaatt
     5161 atttatgtaa ttctcgatta gttcctttag aaaaaaggtt cctttccctt cagactcaaa
     5221 tatatgatcg taaaaagtgg gtttgtaatg tgcttttaaa taaattaccg catagtcatt
     5281 tatattgttt atattactta attcatccat agcatgtgag ttacccataa aaatatcaaa
     5341 catttttacc tggcacgaga acgtttttaa aagagaatcc tttctactga tacgtttttc
     5401 ataagtattg tttttttcaa ttaaattttt aatatttccg gctatactct tgaaatctga
     5461 agaaatatta tattcctgat tgctttcctg cattcttatc aattgtacta tttgctttac
     5521 aggattatta aatcttttta caatataaag aacaataaat ccgcttgcta aaatagagca
     5581 tactataata aattgaatca ttaccaaact tttattaatc tgctgtttaa ccagaatatt
     5641 tgaataaaac ttatagtatt caagttcatt caaaccgcct tccaaaacaa atatatatcc
     5701 gttatccatt ttagtataag cactaatacc gctgccatta agcagtctct cattaaacat
     5761 gacttgcccg gttgattccg gaaacaatag cactccatct ttcgtaaaca cataaaggtc
     5821 ttctgtaaag ccggatctgg ccgattctag tagtactttt acattaatta acgccaccat
     5881 aataaatttc gagttgttgc tttgcttaaa tgcaataggc ataaggtaat tatccgtttg
     5941 agctgcggat gtggaaagat cggcaaaata gcctgcggga tagaatttaa atgaaaaccc
     6001 ttcatctatc tcctgcaccc agaaatcttt caggtatgta ctgttgttaa agaaagtatt
     6061 aaattgcata tcgttgggac ttaacaattg catcggggtt tttataatta tgaatatctc
     6121 gcttatatat ttttcggtta atttgaaatc attaagctca ccttctataa ggccatactt
     6181 ataaggtgtt aaatctgaac ctctaagatc ctggtagaat ttactgaagg ttttcaaaaa
     6241 aatatcattt tttactttat aaaatttact ttccagctca ttaaccagta tttgcaagcg
     6301 ggaatcaaca ttttcagtta tttcagattc tatgcttttc ttgtatatac tattcgtaaa
     6361 aacgtgggaa gatgatataa atattattat taataaaaaa cttgaaaaaa gtctgataaa
     6421 caaggcattt atactaagat atccctttat ttttttgaac atcctgttcc ctcctatacc
     6481 accggctaaa taatgaaaag cctgggggca ccgttcgcat tgccaagcac ttccttatcc
     6541 actttatgta caaaagtgct ggtattctgt ttccaaaata ccggcacttt tcagtcaata
     6601 gaatattaga gactattctt agcgttttac tcttgcattt ccttcaaaga tgctccaaac
     6661 ttgctcttcg tcagcaggtt gagcataatc tgtaaggaat gttgttgcca tcgccccggt
     6721 tgcccatccg aactggatcc atttttcagg ttcccatccc tttagaatac catagagcaa
     6781 gccacctaca aaaccgtctc cgcctccaat acggtcaagg acagttattt cacgaggttc
     6841 aactacatgc caattctcac cttccagcat aattgcaccc cacatatggc tgtttgtgct
     6901 tataacctgg cgcagtgtgt ttgcaaatac tgaagtgttt ggatatgctt ctttaactcg
     6961 ttttatcatg tttttaaagc tatctatttt tgatacaata tcttttcctc ctgcttcagg
     7021 gccttttatt cccaaacaga gctggaagtc ttcttcgttt cctatgagaa catctgaaat
     7081 acttgctatc tcagtaaaaa tatttctaag ttcttcttct ctattcttcc agaatgatgc
     7141 gcgataattc aggtcgaatg aaattctcgt tccatttttc tttgcaaatt tggcaagttc
     7201 aagacaaaat atgcttgttt cgtgtgaaag cgcagctatt agtcctgata aatgtaaaat
     7261 ctgaacacct tccttgacaa agatcctttc aatgtcaaaa tccttaatat cgagggttcg
     7321 gcctacttct ccggcacggt cgttatgtac tctcggtcca cgagaaccat aaccgctgtc
     7381 ggctatattg aactggtggc gatatcccca tggtcctccc tgtggaacgt ccaagccctc
     7441 gtaagacata tggcggcctg caagattgtc tttgataaac ctggcaatgg ggctgtcttt
     7501 cacaaatttt gtcagtactt taacagtaag tccaagatac gaagaaatac ttgctacatt
     7561 agtttctgca cttgtagctt gcattacaaa agtatcacta ctatgaacag gttgtccatt
     7621 taccggagtt atacgtacac ccatgctggt gggcacaacc atagaataag taaaatcttt
     7681 ccgtaattcc atcttaaaaa atcctcctaa caaattatta ttaatttttg cttttttgca
     7741 tttaataaat ttcaaatttg gtttctttac ctgaatattt tgggtaaaca ttaaaaaaat
     7801 aatgcatgtt ttattatacc ctatcttata ttttttttca aactttgaac aactgttgga
     7861 tgcattttcc tcctgttttt cttcaaatat aataaatgca ccccaattgg gatgcattta
     7921 ttatattatg aatagtatga agaagtattt actaaacaaa agggataccc tattttctta
     7981 aaataaataa ttctactctg ttaaaacttg aggttttact tattatctca gttttctaaa
     8041 cgcttcgctc tcaagatatc tcatatcaaa gaaatcaagt ttaatttcag acggatcagt
     8101 tgatgccata tagccttcaa ggtctgagaa aaactgcatg aaaatactat gatttcgcat
     8161 atgacttgcc atgaggaagt gttctttggt ctgttccagt cgtgtcatca gtgcaagtcg
     8221 agccagtttt ctttgaagtg ccatatcctt tatcttttca tatttgcact tacagcaggt
     8281 atcagctata tctatcattt taaaaatggc aagataatct tccgcaaggc atctataatt
     8341 ttcaacttca tagccaaatc gtttcgccag tttcgtgttg cagcgaggat ctgcagaaac
     8401 ctcatcaagc agcgcagagg cttccttggc catcacagct atttcattga tctccctcaa
     8461 ataattcttg cggtctgcat ttataattct tagtgcctca ccagggaata ttcgggggta
     8521 gggtttgcct tcacgaacat agctgtaaaa ataataggac atagtagaca gcaatatgcc
     8581 gtacttagaa agtacaggag taccatcctc cttcttttcg tttctcactt ctgtaataag
     8641 atcaatgagt tcaaaagctc tttgcgcctt atcatattgt gcaccaaaat ttcgtttgac
     8701 atagcgatca gtcatacatt caattgaacc tgttccctta taattccagc tgtagtcagc
     8761 ctgacatatg tgattgcggt catagctttc atcccatgcg gaataggact gcatgccctc
     8821 aaccccttct tctgtgccca tcttcgctaa caaatagata tttcgcaagg gattagtaag
     8881 aaccgtccag tggtaatagc cattccaagg ctttacagta catctgaatc caaggtcggg
     8941 gcgtgttgtt tggaacataa gcttgtcttg gaaatccgaa tatgtccacc aatcgattac
     9001 aacattatca attaaatcat tatcacgcag tgctttaacc atatcttcgg cactttcggc
     9061 ggtatctgct tctccatgac ctataagcat gtcatggtac atgtaaatat ttttgatgcc
     9121 gcggctcttt aaatgcttca aaagttttat tgcatggtct atgaataaat gtttgcggct
     9181 tgcatttcta cattcggtac atttgcacca gggagagcgc tttttataaa tatcctgtgc
     9241 attatgtgca agtccatccc aaacctcatc cagacctaca tggaaactgt cgatgccatt
     9301 tggaattaaa tacctgtcga taatttcatc atatattgag aacattagtt cataggtttt
     9361 gggatttgat gtgcaaaatc cggtcagagt tgcttcaccg ttctcatcct tagcggaaac
     9421 ctcaggatat gtagctggaa tcaatgtatt atgtccataa gagttgaaaa gcggaaatac
     9481 tgccacacca cgtgttttac cataaacaat aagctcaccg aagaagtcct gctcaaacat
     9541 gggcggaagt ttttcgttgt caacccattt attatatgta ggagagaagt aacgtgctac
     9601 tacaggagtt tgcagttcag gatatttttt aataggaaca tataagtatt cgctgactct
     9661 gccgtcgtac tgaacgcacc agcatccata aactgaaaca acaagctgat tcattttcat
     9721 atttgccatg tgatcaacaa catgcttcca atcgtccaga gtcataagat ttgatccgta
     9781 acgcgactcc atgaaatgac cacgtgtttt cagatccggc caatcgagga ttctcatgca
     9841 tggcaatttc aagatattct cttcaagctt cagacactgc ttcagagtta tcactccgta
     9901 atagagtcca agagcaccaa agccggttat ggttaccgcc tctggagtta cttccaataa
     9961 atatccctgc tctggattct tcacattgtc aggagccttc cctactgcta gtctaatagg
    10021 aattgccccc tttgcatcag aggcatcagc attcagcaag cctgtcatat agctgcaaag
    10081 aagttctaca gcagtttctg ccagccgatc atcaggtaag gatgtggctt ctatttggta
    10141 catcgccttc ccaggtttgc caagtacaac ttcatcaccc tcaagggact caaaatgctg
    10201 tggaattggg gaaatataat ttttaataga catatgcacc tctcctttaa ttttattaat
    10261 ttaaaaaatg tacttcatag ttaacaagtt ttaatgcagt gcatttgatt attttttatc
    10321 ctactccata ttgtattatt cttactctat attgcattta ctatatataa cgtttactat
    10381 acataacgtt tactatatat aaagtttact atatattgca tttgctacat tcgccatata
    10441 tcgaagcaat ttggattagt aggcgcttct tccaatacac ctatatatag gcatgtctct
    10501 ttgctatatt actatagata aatgattaaa tatatactga aaatagctaa aacttgcact
    10561 attttcgtca gaaaactgct ccgacagaat ttctctagtg aactcgttca gctaaagctg
    10621 aacatcaggg aatcagatgg agactctact ccaaatgatt taggtcccac ctatagaggt
    10681 gggagtctta actcaataag atgttatatt gaaaaatatg tccaagcaga aaaaaaatat
    10741 tgctaaacat aaaaaaatta tgatataata tcctcataaa tgaggatatt atatgcctcc
    10801 ggcggaattt atgt
//
LOCUS       DAOSDD010000153        10718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2354_length_10718_cov_5.834099, whole genome shotgun sequence.
ACCESSION   DAOSDD010000153 DAOSDD010000000
VERSION     DAOSDD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_10718_cov_5.834099"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            515..883
                     /locus_tag="PK733_12935"
     CDS             515..883
                     /locus_tag="PK733_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOJ11480.1"
                     /translation="MTESLEDERWLIFLDDSPEEVLKMAMEKDNVIAKAEETLKKLGS
                     INEIRAECEYREKAIRDELSRIQGAREEGETIGVQKGERRKAELTARNLLSMNISIEI
                     ISKATGLSIEEINKLSEGGM"
     gene            1206..2195
                     /locus_tag="PK733_12940"
     CDS             1206..2195
                     /locus_tag="PK733_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOJ11481.1"
                     /translation="MGFKTLKYEHIFEPEKYFPQCHASTLLKLKNGDILSAWFGGTHE
                     DHEDVAIWYSRRKEDKWSEPVKVADEEGIPCWNPVLFSDEKDNIYLFYKVGKNPRSWY
                     TMLIISEDMGHSWSQPKELIPGDVGGRGPVKNKAIILSDGTWLAPASIETDTLWDAFA
                     DISRDEGKTWIKSGMVPLDHGKLQGKGVIQPTLWESEPGKIHMLLRSSEGFIYRSDSS
                     DNGLTWCEAYATGVPNNNSGIDLVKMEDGKLVLIYNPIRGNWGARTPVVCCISSDNGE
                     TWSENFILDHNENPADKVDGEFSYPAIIAEGNRVYITYTWKRKTVAYWELEVL"
     gene            2258..3544
                     /locus_tag="PK733_12945"
     CDS             2258..3544
                     /locus_tag="PK733_12945"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11482.1"
                     /translation="MSKIVMIGVGSISFGAKTIGDLIHFKDILKGSIISLVDIDEEKL
                     RLMKGLIDKMNKDAGMPFKIEASTQRRDVLTGADFVVTSPAIKREELWKQDWTIINRA
                     GIKQTYGENGGPGSLALTMRNIPMILSIAKDVEELAPNAWLINFTNPEARICMALDRY
                     SSVKFVGLCHQIYESYKTIHTVLGIEFSDIDVKAAGTNHLTWIYDIRRKSTGRSIYKE
                     FSDKLKEMPENYEPMSRKLFSMFGMYPTSGDHHLAEFMPFGWEYQGLLGRDFESRKKK
                     IDDTMEWLRGVNNETRQVGEIVSGKSTESVADIIAAMTTGDNHYEVSLDIRNNGCIPN
                     LPDNAIVEVPGVVSGDKVRGLRMDPLPEGIVELIRRQITVQSLSVEAAITGSRDKALQ
                     AFILDPVVDNISTAEKVLDELIQANKEYIHQGFVCA"
     gene            3739..4050
                     /locus_tag="PK733_12950"
     CDS             3739..4050
                     /locus_tag="PK733_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11483.1"
                     /translation="MPANDMDYWIPKNITPERQDNIRTWLNTFIGNAELTLDAGFRPA
                     AADIPSFIDYHSKVSEVVAKIIIGELPVSAWDQCLEGWYKAGGEEYVKQMNEYIASRR
                     K"
     gene            4122..4688
                     /locus_tag="PK733_12955"
     CDS             4122..4688
                     /locus_tag="PK733_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11484.1"
                     /translation="MQLGCTLCFKFISSFGIGAGIGDINNKNTAECFENLKKIYRPDN
                     IINGYNRPYLLPNLWISDKITSNTSQYIDLLFDDRKEINEIIIFFNSNLNIERNNLRP
                     GLWRKSDCEMPRELVKEYKIFTLNDDAQPSQLLIHEKNNKGRYVKHKIEYCNNSGLNC
                     TVKANGIRIEVIDTWGNPYAEIFEVRIY"
     gene            complement(4989..5429)
                     /locus_tag="PK733_12960"
     CDS             complement(4989..5429)
                     /locus_tag="PK733_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11485.1"
                     /translation="MKKKDIFTKVLAIAGTVLVWLPILFTILTSVIGTISAHIFRFDY
                     LMPAELFPIALIGALLLIWAAKRAHLHWKLITWSLGAMVTFLVGGQVIAVISGLASGA
                     IEPAGWVWALVVTSIALYSLTLIVVCIAGVLLVKKLYFFHSPQP"
     gene            5837..6823
                     /locus_tag="PK733_12965"
     CDS             5837..6823
                     /locus_tag="PK733_12965"
                     /inference="COORDINATES: protein motif:HMM:NF041162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOJ11486.1"
                     /translation="MSFNDNLVPQYSLSPKAAKNLSHSTISVPMMEAITPRWLLTFLP
                     WVSVEAGVYRLNKMKKCNEIRDGEDDYREKLPELCSSHLGEHEIPQTFPDYEEKPREY
                     TLSLVQTILRVNGQVTDIFNSPINQLQQQMRLTIEAMKEQQEWEIVNNKDFGLINSVT
                     PSMRVLSRRGTPTPDDMDELLAKVWKKPAFFLAHPRAIAAFGRECTCRGVPPATVNLY
                     GSPFITWRGVPIVPCDKLLVDGKSCNHSSTGKTNILLMRVGEKDQGVIGLHQPGIQDE
                     SAVPSLSVRFAGIDNRGIVSYVITLYFGAAVLTYDSLGMLENVEVGRYHEYE"
     gene            7008..8249
                     /locus_tag="PK733_12970"
     CDS             7008..8249
                     /locus_tag="PK733_12970"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOJ11487.1"
                     /translation="MSTFYYKPGLDVYSIRKDFPILQRRINGKPLVWLDNAATTQKPI
                     CVMSALNRYYSEYNSNIHRGAHTLAKLATNAYENARQKIQNFIGASSTEEIIFVRGTT
                     EAINLVADSFGGMNIQTGDEIVLSMMEHHSNIVPWQKLQQAKGAVIKVIPINDRGEVI
                     LDEYEKLLTPRTRIVSITHVSNVLGTINPVCKMIEMAHKHGAYVLIDGAQSVPHMGVD
                     VRALDADFYAFSGHKIYGPTGIGVLYGKKVLLEEMPPWQRGGGMIKNVSFNKTTYNSL
                     PYKFEAGTGNIADAVGLGAAIDYIQGIGMDNVERHEKGLTVYAMEKLYQIPGLHIIGT
                     APEKTGVISFVINGVLPENAAQYLNLDGIAVRSGHHCAQPALHRYGLTSSIRASFGVY
                     NTMEEVDCLVSSVLKIAKYYN"
     gene            complement(8317..>10718)
                     /locus_tag="PK733_12975"
     CDS             complement(8317..>10718)
                     /locus_tag="PK733_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11488.1"
                     /translation="YIEANLYGIIQAYIKFIGKQKNIINFRPNILHKRQVNSGGYIVT
                     FKEVCGYRDEVWGTLQYDAGINGIVPEPGKGITLIVRRIKSGSTDTIVNEKSYPSKTD
                     ERGNFHLSGVDLQKDDEVFIRLQERGKTNYVKSAITYPTFPFDKVIVLEADFFNDYIK
                     GYVPSAIVKDWNTTQSEQQSEQQKEIVFDLKKSLNSSLKVNGVPVTLDSKGFFNLTGT
                     NVLPQDSYSCELAFEGWIVKSSPVKPTVDFGAHSIRLPVSYERILENSKPADVSKLNE
                     TVIITNNRGSKVYTEDAAFTVSGYTQAATVCYLINPKTGLPIMQAMGLPTQTQLVKLY
                     AVQGAEEGSFGASYFTNTIIKKWLWEPKPGQIQIQEPDIEQLPESKPEQILDPKTGQL
                     LDSMNEQIPDQKTRQLLDPKVDQMPSSITRFGVTQVNPNLVAPSSTTNTTNLPAPAST
                     TNATNLLAPSSTTNITNMPAPSSTTNTNVEPGTLNIIPIRPIDPDLSKYLPSLPQHSF
                     TLSYDDNFDKYDDYNYYNNYTSKLENYSEQTDGTQTIKWDDKIAITYEGAQIILEIKD
                     DRRGSNADSKFETLSAEWLNKDIEGMLNHYMEKMGGSLVFPMPDEMNRTGGISNIGML
                     PSWSSQAAQKMVDAGIMDLSSGQVFKSGTVTRSECAAYLVHVFGIMSDMKKPVFLDIV
                     PANPYIPEINAAVSYGIINGYSPEKFGPGDPVTREQMAVMVMRGLKKKLGSSLSIPAT
                     GKNFADKDKFSSWSGSAIYEISALKIMNGYPDGRFGAKDNITFNEMAVMLDNLNGFLN
                     K"
BASE COUNT         3454 a         1882 c         2276 g         3106 t
ORIGIN      
        1 aggattggca agcccaatcc agcagtagtg gggttggata acccggaggt gtaccattgg
       61 aattaccata atttcatcgg gaacccgaag cccgctattt caatagcggt gtagttcaca
      121 ggtttgataa ttatcaatta gttttacgga tggtgaaatt caagatatta tcggacaata
      181 tgatttttct aaggaccatg cagaattata taaaaaacgc gaagtattag ataaaatttt
      241 ctcctattat tttgagggcg agaaatattt attatctgca actatttttg atattgatac
      301 cggcgattac gtttatagaa ataatattcc gagggatatc aaaggtttga gagattccca
      361 atggtttttg catactttag aaagcaaagg acacaaggat accgaattga tgtatagtta
      421 tgatggagaa cactggatgc atactgccag aaaatcatta atggagagaa actaatcacc
      481 tgagtatggt tttaaccaat taaatttttc cgagatgacg gaatcactcg aagatgaaag
      541 atggcttata tttttagatg attcccctga ggaggtgctg aaaatggcta tggaaaaaga
      601 taatgtgatt gccaaagcag aagaaacctt aaaaaaactt ggaagcataa atgagatacg
      661 ggcagaatgc gaatacagag aaaaggcaat aagagacgag ctatccagaa tacagggagc
      721 tcgggaagag ggtgagacaa taggggtaca aaaaggagag agaagaaaag ctgagcttac
      781 tgcaagaaac ctattaagca tgaatattag tattgaaata atatcaaaag caacaggatt
      841 atcaatagaa gaaataaaca agttaagtga agggggaatg taaataattt tgtgtatggc
      901 gattttatgt aaacatttct gctgaattat acgaaacact acaatgctta tacaggttcc
      961 atctatactg aaaaatgcat ataaaatagg aattcagatg gattctgcta tactatactc
     1021 tatatctgga actatcagat catatattgt gtgccctcag aaatcaatat cggatcctat
     1081 acagttgggt gtagataggt aaaaggagaa aaatataaat gtgcacctaa ataaaaacga
     1141 aaagcaaggc atatgcaaaa tttttataat acaattaata aataatcagt agaaaggtga
     1201 gtattatggg atttaaaaca ttaaagtatg agcatatttt tgaacctgaa aagtattttc
     1261 cacagtgcca tgcatctaca ttattaaaat taaaaaatgg tgatatactt tcagcatggt
     1321 ttggcgggac tcatgaagat catgaagatg tagcaatctg gtattcaagg agaaaagagg
     1381 acaaatggag tgaacctgtt aaagtagctg atgaggaagg tattccgtgc tggaatccag
     1441 ttttgttcag tgatgaaaaa gacaacatct atttgtttta taaagtaggg aaaaacccga
     1501 gatcctggta tacaatgtta attatatctg aagacatggg gcactcctgg tcgcaaccaa
     1561 aggaattaat tccaggggat gtaggaggaa gaggtcctgt taaaaacaaa gccataatac
     1621 tttctgatgg tacctggctt gcacctgctt ctatagagac tgacactctt tgggatgcct
     1681 ttgctgatat ttccagggac gaaggcaaaa cctggataaa aagtgggatg gttcctttgg
     1741 accatggcaa acttcagggt aagggtgtaa tacagccgac tttgtgggaa tcagaaccag
     1801 ggaaaatcca tatgctgcta agaagcagtg aaggctttat atatcgcagt gattcaagtg
     1861 ataacggatt aacatggtgt gaagcatatg caacaggagt tccaaataat aatagcggta
     1921 ttgaccttgt gaaaatggaa gatggtaagc tggttcttat ttataatcct ataagaggca
     1981 actggggagc aaggacacct gttgtatgct gcatttcatc cgacaatggt gaaacctgga
     2041 gcgaaaactt tatactcgac cataatgaaa accctgcaga caaagtagat ggagaattct
     2101 catatcctgc aataattgct gaaggaaatc gtgtatatat tacatataca tggaagcgaa
     2161 agacagtggc atactgggaa ctggaagtat tatagataaa ataatttggt ttatacagca
     2221 tattagcaga taacagcata ttaatggagg taataaaatg tccaaaatag taatgatagg
     2281 cgtgggcagt ataagttttg gggcaaaaac aataggggat ttgatacact ttaaagatat
     2341 tttaaaaggt tctataattt ctttggtgga tatagatgaa gaaaaactga ggctcatgaa
     2401 aggtcttatt gataaaatga acaaagatgc aggaatgcct tttaaaatag aagcaagtac
     2461 acagagaaga gatgtactta caggtgctga ttttgtggta acgtcccctg caattaagag
     2521 agaggaatta tggaaacagg attggacaat aataaatagg gcagggatta aacaaacata
     2581 tggagagaat ggaggaccgg gaagccttgc tttaactatg cgcaacattc ctatgatatt
     2641 atctatcgcg aaggatgtag aggaactggc acctaatgca tggctaatta attttactaa
     2701 ccccgaggcc aggatatgta tggctcttga cagatattca tctgtaaagt ttgtagggct
     2761 atgccatcag atttatgaat cctataaaac tatacataca gtgcttggca ttgaatttag
     2821 cgacatagac gtaaaagctg cgggaacaaa ccatttaaca tggatatatg atatacgaag
     2881 aaaatctacc ggaagaagta tatacaaaga attctctgat aaattaaaag aaatgccgga
     2941 aaactatgaa ccaatgtcaa ggaagctttt ttctatgttt ggtatgtatc caacctcggg
     3001 agatcatcat ttagctgaat tcatgccatt tggctgggag tatcaggggt tactgggtag
     3061 ggattttgaa tcaagaaaga aaaaaataga tgatactatg gagtggctta ggggagttaa
     3121 taatgaaacc cgacaagtag gggaaattgt atcgggcaag agtacggaaa gtgttgccga
     3181 tattattgca gctatgacaa caggagataa tcattatgag gtttctcttg atattagaaa
     3241 taatggttgt atacctaatc tccctgataa tgctattgtt gaagttcccg gagttgtatc
     3301 aggggataaa gtgcgcgggc ttagaatgga tccactgcct gaaggaatag ttgaactaat
     3361 tcgccgtcag attactgtac agtccctgtc agtggaagct gcaataacag gaagccggga
     3421 taaggcactc caggctttta tccttgatcc ggttgtagat aatatatcta cagcagaaaa
     3481 agtgctggat gaactgattc aagcaaacaa ggaatatatc catcaagggt ttgtatgtgc
     3541 ttaagtttgc ggcgataatt caggacaatt gctataatac gttttgctta tttaattgcc
     3601 agaaatgaag catgaatttg aaatcaagag gaataaggag ctatagagat tccttaaaaa
     3661 tattttctca ggatggggtt gaacttacac aagctcagta ttgtatagta gacttggaaa
     3721 ggtgggttta aattagtaat gcctgcaaat gacatggact actggatacc gaaaaatata
     3781 acgccggaaa ggcaggataa tatccgtaca tggcttaata cttttatagg taatgctgaa
     3841 ttaactctgg atgcaggatt ccgcccggct gcagcagata ttccttcatt tatagattat
     3901 cattctaaag taagtgaagt tgttgccaag attattattg gagagcttcc ggttagtgcc
     3961 tgggatcaat gccttgaagg atggtacaaa gcaggcggcg aagagtacgt aaaacaaatg
     4021 aatgaatata tagcaagcag aaggaaataa gtatgcccac attgtatcta catgatttgt
     4081 gtgatggcgg aaaaattata gaaaaattta aagcttaaag tgtgcagctg ggctgcacac
     4141 tttgctttaa gttcatatca agttttggta taggggctgg cattggggat ataaataata
     4201 aaaacactgc agaatgtttt gaaaatctta aaaaaatata tagaccggat aatataatta
     4261 atggctataa tcgtccatat cttttgccaa acctctggat atctgacaaa ataacatcaa
     4321 atacttccca atatattgat ttgctatttg atgatagaaa agaaataaat gaaattatca
     4381 tatttttcaa ttcaaacctt aatattgaga gaaacaatct cagacctggc ttatggagga
     4441 aaagtgattg tgagatgccg cgggaactgg taaaagaata taaaatattc acgttgaatg
     4501 atgatgcaca gccatctcag ctgttgatac acgaaaaaaa caacaagggc agatatgtta
     4561 agcacaagat agaatactgc aataacagtg gcttgaattg tactgttaaa gcaaacggaa
     4621 taagaattga ggttattgat acctggggga atccctatgc agagatattt gaggtaagga
     4681 tatattaaga gagggctgca gtgttattta caatttatac aagtttggca ttattttaag
     4741 ataaattatt atttaagaag cttactgtaa acaagtactg tgtaggggaa tattggtaat
     4801 ggaacatgaa atattattcc atcggaatgg gatggtacct gagttttgaa caggagctga
     4861 gcattttcgg agctaaaata acttctattg aggcggaact ggttgtttct ttaaacaccc
     4921 gagacagccc tgcgcatagt ggtgatggtt tcctctaccg agtaaaatca ttttttgcct
     4981 tatatatatt atggctgagg agagtgaaaa aaatatagtt ttttcactaa taacacacca
     5041 gctatacaga ccacaataag tgtaagcgaa taaagagcga ttgaggtgac caccagcgcc
     5101 caaacccagc ctgctggctc aattgctcct gatgcaagtc cgctgattac agcaatgacc
     5161 tgaccgccaa caagaaatgt taccatagcg cctaaactcc aggtgatgag tttccaatgt
     5221 aaatgtgcac gttttgcagc ccagataagc agcagggcac cgatcagggc aataggaaag
     5281 agctcagccg gcatcagata gtcgaaccga aaaatgtgtg cagatatcgt tcctattacg
     5341 gatgtcagaa tggtaaacag aatcggaagc catacaagta ctgtgcctgc tatggctaaa
     5401 accttagtga aaatatcctt cttcttcatt ctcaaaacct cctcagcact taaaagaatc
     5461 aatcaaacaa ccactcaaaa caaccactca aaagaatcaa tcaagtcaac aattcattct
     5521 atatagaaca ggttttaaaa catttaaaac ctgcttctgt taccttgatg taacttacga
     5581 tttgcaaatt cttcattagt tctttcaaat cgtaaattat atagagtatt tcttacttcc
     5641 atattatcac ttatctttaa cacttacaat aatatttgtc gaagctacag tatctcttga
     5701 ttcacctatc tatacttgtt acgttaatca cttctcatat ttcacaagca tatattgtat
     5761 agatataaga gttttttgtt aatcaaaaaa caatttctaa tatatagaga gatgttatat
     5821 atgagaggtg ttaagaatga gttttaatga taatttggta cctcaataca gcttaagccc
     5881 taaagctgca aagaatttgt ctcacagtac aatatctgta cctatgatgg aagctattac
     5941 cccgcgatgg cttcttacat ttcttccctg ggtttccgta gaagcgggcg tttaccgttt
     6001 gaataagatg aaaaaatgta atgaaataag ggatggagag gacgattacc gcgaaaaact
     6061 gccggagcta tgttcgtctc atttaggaga acacgaaatt ccgcaaacct ttccggatta
     6121 tgaagaaaaa ccaagagaat acacattgag tttagtacag acaattttaa gggtaaatgg
     6181 ccaggttaca gacattttca attcacccat taatcagctc cagcagcaga tgagacttac
     6241 catagaagca atgaaagagc agcaggaatg ggaaattgta aataacaagg attttggact
     6301 gatcaattca gtcacaccgt ccatgagggt actatcaaga agggggacac ctactcccga
     6361 tgatatggat gaattgcttg ctaaagtatg gaagaaacct gcattctttt tagcacaccc
     6421 gagagcaata gctgcttttg gccgggaatg tacctgtaga ggagtcccgc ctgcaacagt
     6481 caacctctat ggttcaccct ttatcacctg gaggggagtt cccatagttc cttgcgataa
     6541 attgctggtt gatggcaaat cctgcaatca ctccagtact ggaaagacga atatcctatt
     6601 aatgagggtc ggcgaaaaag accagggtgt tatagggttg catcaaccag gaattcaaga
     6661 tgaatccgct gttccaagtt tatcggttcg atttgccgga attgacaata gagggattgt
     6721 gtcctatgtt attaccttgt atttcggggc agctgtttta acatatgact cattaggtat
     6781 gctggaaaat gtagaggtgg gaagatacca tgaatatgaa taaaccggaa gatattaaaa
     6841 gatcattttt taagactaaa gagtatttcc caggttatgc gaactcaggt tatctgaatt
     6901 caggttatac aaattcaggt cataccggag ataattcatt ttacttttta ccggattatc
     6961 aatcagctcc ttatactcct tatacttcca atactcatac aaaccaaatg agtacctttt
     7021 attacaaacc tggtcttgat gtgtactcta tccgaaaaga ctttccgatt ctgcaaagaa
     7081 ggattaacgg aaagccactg gtgtggcttg ataatgctgc aacaacccag aaacctatat
     7141 gcgtgatgag tgcattaaac cggtattata gtgagtacaa ttccaacatt cacaggggtg
     7201 ctcatacatt agcaaagctt gcgaccaatg cctatgaaaa tgccagacaa aaaatacaaa
     7261 actttattgg ggcatcgtct actgaagaaa ttatttttgt acgcggaaca acagaggcaa
     7321 tcaaccttgt agctgatagt tttggaggaa tgaatataca aacaggtgat gagatcgtac
     7381 tgtcgatgat ggaacatcac tccaacattg taccctggca aaagctgcag caggccaaag
     7441 gagctgttat taaagttatt cctatcaatg accgcgggga ggtcatactg gatgaatacg
     7501 agaaactttt aacaccccgt acccggattg tgtctattac gcatgtgtcc aatgtcctcg
     7561 gaacgataaa tcctgtatgt aagatgattg agatggcaca taaacatgga gcttatgttc
     7621 ttattgacgg agcacaatct gtccctcata tgggtgttga tgtaagagca ctggatgcag
     7681 atttttatgc tttttcaggg cataagatct atggtcccac aggaattggt gtattatatg
     7741 gaaagaaagt tttactggag gaaatgccgc cgtggcaaag aggcggtggt atgataaaaa
     7801 atgtaagctt caataaaact acttataaca gcctgcccta taagtttgaa gctggtactg
     7861 gcaacattgc agatgcagta ggtttgggtg cagctattga ttatattcaa ggaattggaa
     7921 tggataatgt cgaaaggcat gagaagggat taacggttta cgctatggaa aaattgtacc
     7981 aaataccggg tttgcatatt atcggtacag ctccggaaaa aacaggagta atttcatttg
     8041 tgattaacgg tgtattaccg gaaaatgctg cacagtatct aaaccttgat ggaatagctg
     8101 tccggtcagg ccatcactgt gctcagcctg ccctgcacag atatggctta accagttcta
     8161 tccgcgcatc tttcggtgtg tataacacta tggaagaagt tgactgcttg gtaagttccg
     8221 ttcttaaaat agcaaaatat tacaattagt ggtgcttaac ttgttgaata atgcaataag
     8281 aaatcatctg gaatatctta ccttaacctg cgccagctac ttgttcaaaa atccatttaa
     8341 attatcaagc ataaccgcca tttcattgaa ggtgatatta tcttttgcac caaagcgccc
     8401 gtcaggataa ccattcatga ttttaagcgc agaaatttca tatatggcag aaccagacca
     8461 tgagctgaat ttatccttat cggcaaagtt cttaccagta gctggtatgg ataaactgct
     8521 tcccaatttc ttctttaacc ctcgcataac cattacagcc atctgttctc gtgttacagg
     8581 gtcaccggga ccgaattttt cgggactgta gccgttgatt atcccatagg acacggcggc
     8641 gtttatttcg ggaatatacg gatttgccgg tacaatatcg aggaatacag gctttttcat
     8701 atccgacatt attccaaaaa catggacaag ataggctgcg cactcgcttc gggtcactgt
     8761 gccggattta aacacctgac ctgagcttag gtccataatt ccagcatcaa ccattttttg
     8821 agctgcctga cttgaccatg aaggaagcat tccgatattg gatatgccgc ctgttctgtt
     8881 catttcgtca ggcatgggaa acacaagact gccgcccatt ttttccatat agtggttaag
     8941 catgccttct atgtctttgt tcaaccattc cgctgaaagc gtttcgaatt tggaatcggc
     9001 gttgctaccc ctgcggtcat ctttgatttc taaaatgatt tgagcacctt cataagtgat
     9061 ggcaatctta tcatcccact ttattgtctg agtgccatct gtttgctcac tgtagttttc
     9121 caatttgctt gtatagttat tgtaataatt gtaatcatca tacttgtcaa aattatcatc
     9181 gtagctcagt gtaaatgaat gttgcggcaa acttggcagg tacttactta aatcgggatc
     9241 tatgggccga attggaataa tatttaaggt tccaggttca acattagtat tagtagttga
     9301 agatggtgcc ggcatattag taatattagt agttgaagat ggtgccagca aattagtagc
     9361 attagtagtt gaagccggtg ctggtaaatt agtagtatta gtagttgacg atggtgctac
     9421 taaattggga ttaacctgag taaccccgaa tcttgtaatc gatgaaggca tttggtccac
     9481 cttcggatcc agcaactgcc tagtcttctg atctggtatc tgttcattca tagaatctaa
     9541 gagctgccca gtctttggat ccagtatctg ctccggcttc gactccggca gctgctctat
     9601 atccggttct tgtatctgta tctgcccagg tttcggctcc caaagccatt tctttattat
     9661 ggtattggta aaataggatg caccaaaact gccttcttct gcaccttgca cggcatatag
     9721 cttcaccagc tgtgtttgtg tgggaagccc catagcttgc ataataggca gacctgtttt
     9781 tgggttgatt aaatagcata cggttgcagc ctgtgtataa ccgctgacgg taaatgcagc
     9841 atcctccgta tatacttttg aacctctatt gtttgtaatg atgactgtct cattaagttt
     9901 tgacacatct gcaggtttgc tgttttcgag tatcctctca tatgaaaccg gaagtcttat
     9961 tgaatgtgca ccaaaatcaa ctgtaggttt tacgggactt gatttcacaa tccagccctc
    10021 gaatgcaagc tcacaggaat acgaatcctg aggtagtaca ttggttcctg ttaaattgaa
    10081 aaaacctttg ctgtccagag ttaccggcac tccgtttacc tttagggagc tgttcagact
    10141 ttttttgagg tcaaacacaa tttccttctg ctgctcggat tgttgctcag actgagtggt
    10201 attccagtcc tttactattg cggaaggtac atagcctttg atatagtcat taaaaaagtc
    10261 agcttccaac acaattacct tatcaaaagg gaatgtaggg tatgttattg ctgattttac
    10321 ataattagtc ttgcctctct cctgcagacg gataaatact tcatcatctt tttgtaaatc
    10381 gacaccagat aaatgaaagt tacccctttc atctgttttt gaaggatagc tcttttcatt
    10441 gacaatggta tcggttgaac cgctttttat tcttctgaca atcagagtga tgcctttacc
    10501 aggctccggt acaatgccat ttattcctgc atcatactgc aaagtgcccc aaacctcatc
    10561 ccggtaaccg caaacctcct tgaaggtcac aatataccct cctgaattta cctgcctttt
    10621 atgcagtata ttaggtctaa agtttataat atttttttgc ttgccaataa acttgatata
    10681 tgcttgaatt ataccataca aatttgcttc tatatatt
//
LOCUS       DAOSDD010000154        10627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2375_length_10627_cov_5.447503, whole genome shotgun sequence.
ACCESSION   DAOSDD010000154 DAOSDD010000000
VERSION     DAOSDD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10627
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_10627_cov_5.447503"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK733_12980"
     CDS             complement(<1..643)
                     /locus_tag="PK733_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOJ11489.1"
                     /translation="MAKDILFGEEARRALERGINQLTDTVKITLGPKGRNVVLDKKFG
                     SPAITNDGVTIAKEIELEDKFENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIVREG
                     MKNVAAGANPMIVKKGIAKAVDVAVNEIKVISQKIKGKDDITRVASISANDEVIGSLI
                     ADAMEKVTNDGVITVEESKTMGTNLEIVEGMQFDRGYVSPYMITDTEKMEAILD"
     gene            complement(697..981)
                     /gene="groES"
                     /locus_tag="PK733_12985"
     CDS             complement(697..981)
                     /gene="groES"
                     /locus_tag="PK733_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ11490.1"
                     /translation="MIIKPLGDRVVIKMVETQETTKSGIVLPGSAKEKPQIAEIVAVG
                     PGGLVDGKEVKMEVKVGDRVIISKYSGTEVKVDNQEYTILRQSDILAVVD"
     gene            complement(1301..1564)
                     /gene="hisE"
                     /locus_tag="PK733_12990"
                     /pseudo
     CDS             complement(1301..1564)
                     /gene="hisE"
                     /locus_tag="PK733_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
     gene            complement(1724..2257)
                     /gene="hisI"
                     /locus_tag="PK733_12995"
     CDS             complement(1724..2257)
                     /gene="hisI"
                     /locus_tag="PK733_12995"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOJ11491.1"
                     /translation="MIGEEGRVSCMPEYEFKQKSDCKSDRKSDRKSDCKSDCKADCKS
                     DCKVSYDLDRVLNRKMDCNSDNKLKFDCRISELIFNRDGLIPAIVQDIYSNDVLMLAY
                     MNEESFTKTLQTGMAHYWSRSRKKLWLKGETSGHYQYVKSINIDCDGDTLLLKVDQKG
                     AACHTGHRSCFFRTIEL"
     gene            complement(2254..3012)
                     /gene="hisF"
                     /locus_tag="PK733_13000"
     CDS             complement(2254..3012)
                     /gene="hisF"
                     /locus_tag="PK733_13000"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ11492.1"
                     /translation="MEGKRIIPCLDMKDGRVVKGVNFSNIKDAGDPVEVAHAYNEAGA
                     DELVLLDIAATIEGRMTLLDIVSKVAAQVDIPFIVGGGIGSIEDIERVLKAGANKISI
                     NTAAVKTPQLISEAVERFGRNSVIIAIDAAEKADHTGWEVYIDGGNTNSGQDAIQWAK
                     EVEKLGAGEILLTSKDMDGTKNGYDIELTRAVSESVNIPVVASGGAGTMEHFYRVLTE
                     GRADAALAASLFHFGEIKIMELKKYLKEKGVIVK"
     gene            complement(3120..3833)
                     /gene="hisA"
                     /locus_tag="PK733_13005"
     CDS             complement(3120..3833)
                     /gene="hisA"
                     /locus_tag="PK733_13005"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOJ11493.1"
                     /translation="MIIYPAIDIIAGRCVRLIQGQFDKETVYSDNPVEIALKWEQLGA
                     RYLHVIDLDGARTGQPQNTTVAKEMAAKLGIPVQLGGGIRTLDTIEILLSKGIQRVIL
                     GTSAVENPDFVKEALKLFSENVAIAIDAKDGMVAIEGWAKTSEFTAIGFAKKMEELGA
                     RTIIYTDISKDGMLSGPNIKATAEMAEAVNMEVIASGGISSIDDIKKLKEIGVSGAIV
                     GKALYTGDMDLKEALKAAE"
     gene            complement(3934..4821)
                     /locus_tag="PK733_13010"
     CDS             complement(3934..4821)
                     /locus_tag="PK733_13010"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOJ11494.1"
                     /translation="MSKLINDTNFIKLPLFKKGKVRDVYEFDNNLLIIVTDRISAFDV
                     IFPTLIPNKGKVLNSISEFWFDYTKDIIGNHMVTTKVDEFPGDLSRFKEELEGRAMLV
                     KKAEMVQAECIVRGYLEGSGLKEYQKTGAICGIKLPSDLRQCEKLPEPIFTPTTKADE
                     GHDESVTFEELANTIGFELANKLRDITISLYLKASKYAESKGLILADTKLEFGFLDGN
                     LIVADEMFTPDSSRFWTMEDYAPGRPQKSFDKQYVREYLESINWNKQPPAPELPEDVV
                     KNTEAKYIEAYERITGNKI"
     gene            complement(4927..5514)
                     /gene="hisB"
                     /locus_tag="PK733_13015"
     CDS             complement(4927..5514)
                     /gene="hisB"
                     /locus_tag="PK733_13015"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOJ11495.1"
                     /translation="MDRKAYIERKTNETNIKISINIDGEGKSSINTGIGFFDHMLDLF
                     AKHGLFDLQIQAEGDLNVDCHHTVEDAGIVLGQAIKEALGDKKMIRRYGLSYVPMDEA
                     LAMVSLDLSGRPFLVYDGGFTSEKIGNLNTQMIEEFFRAAAFNSGITLHIKVLYGKND
                     HHKTEAIFKAFGRALDEATKQDERIKGVMSTKGIL"
     gene            complement(5737..6795)
                     /gene="hisC"
                     /locus_tag="PK733_13020"
     CDS             complement(5737..6795)
                     /gene="hisC"
                     /locus_tag="PK733_13020"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOJ11496.1"
                     /translation="MSKYWSERVKLLEPYVPGEQPKDKKYIKLNTNENPYPPSPKVLD
                     AIIKSADESLRLYPDPDCIELRKAISSYYKLNEKQVFPGNGSDELLAFSFMAFFNPGD
                     PILFPDITYSFYPVYCSMFNIDYKLIPLDKELSIPIDRFFAKNGGIIFPNPNAPTGKY
                     LPVEAIRNILEHNCDNVVIVDEAYIDFGGDSVIGLIDEYPNLLIIQTFSKSRSLAGLR
                     LGFALGNENLIDALNMIKNSINSYTIDRLAIKAGIEAIKDEDYFQTTKRRIISTRERV
                     TEALKNMGFRVVDSKANFIFITHPDKEAEWLFISLKEKGVLVRYFKKPGIDNFLRVSI
                     GSEEEMEVFLTIIQGLVL"
     gene            complement(7108..8400)
                     /gene="hisD"
                     /locus_tag="PK733_13025"
     CDS             complement(7108..8400)
                     /gene="hisD"
                     /locus_tag="PK733_13025"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ11497.1"
                     /translation="MIGIFKAGGKEVEAFINKLAERNFKGKDTVNRVVAEILENVKTK
                     GDSAVIEYTKKFDGVDIEAEDLKVGSSEIDEAYKVVNSELLAVIRRAKDNIEKYHQKQ
                     LENSWISTEKNGVLLGQLYRPLEVVGVYVPGGTAPLVSSVLMNLIPAKVAGVDRVVMV
                     TPPGRDGKINPAILAAAKETGVDEIYRIGGAQAIAALAFGTETIPKADKIAGPGNIYV
                     ATAKRMVYGYCDIDMFAGPSEIAVVADENANPKYVAADLLSQAEHDIMASSILLTTSE
                     KLAGQVKEEIERQYRYLDRNDIIKKSLEEYGAAIIVENIEAAVEIVNKIAPEHLELYI
                     KEPFSILGSVRNAGAIFLGSFSSEPVGDYYAGPSHVLPTGGTARFFSPLNAGDFIKKT
                     SIISYTEKALLDAKDDIVRFAEAEGLSAHANAIKVRFE"
     gene            complement(8514..9152)
                     /gene="hisG"
                     /locus_tag="PK733_13030"
     CDS             complement(8514..9152)
                     /gene="hisG"
                     /locus_tag="PK733_13030"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOJ11498.1"
                     /translation="MNYLNIALAKGRLGDTAIELFESIGIDCTEIKSGSRKLIFTDEN
                     NKIKFFLVKPSDVPTYVEYGAADIGIAGRDTLLEEGRNLYEMLNLKFGACRMVLAGPA
                     ELQDKLNRLTNKRVATKYPRIAREYFEHRRKESIEIIKLNGSVELAPLVGLAELIVDL
                     VESGRTLKENNLVIFDTIEDISARLVVNRVSMKMKSEKITWIIDRLRKEIEK"
     gene            complement(9266..10525)
                     /gene="hisZ"
                     /locus_tag="PK733_13035"
     CDS             complement(9266..10525)
                     /gene="hisZ"
                     /locus_tag="PK733_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOJ11499.1"
                     /translation="MPKWKIYTPEGVQDILIDECYIKRNIESNIREVFRKSGYMEIET
                     PTLEFFDTFSVDEDSTPQEMMFKFFDHQGRILVLRPDLTIPVARVAASKFKVAKYLLK
                     IFYVGNTYRYNESGGGRQNEFTQAGVELIGSATPEADAEVIATAINALKAAGMENFQI
                     DIGQVEFFKGLMEETGLPEQDVEQMRILIDRKDFIGIEEIINHCNIAEELKELILDLP
                     RLFGTIDVIDRVEKIVKEKRSLDALNYLRNILGILEDHGLGKYVFIDLGMVQSLNYYT
                     GIIFRGFTHGVGFPILSGGRYDTLVEKFGAGRPATGFSIGVNLVMNALERQKTNVKKQ
                     EIDSLICYEKEGRKTAFVICEEMRKQGLVIEMDITMAGMDYISSYAKAMNIGGIIRVV
                     NSEIIEIYNLETGEMQHVTVSELISRS"
BASE COUNT         2957 a         2412 c         1512 g         3746 t
ORIGIN      
        1 catccaggat agcttccatc ttttctgtat ctgtaatcat gtaaggtgat acataacctc
       61 tatcgaactg cataccttca acaatctcaa ggtttgttcc cattgttttt gattcttcta
      121 cagttataac tccatcattt gtaactttct ccatggcatc agctataaga ctgccaatta
      181 cttcatcatt tgcagaaatt gaagcaactc gtgtaatatc atctttacct ttaatttttt
      241 ggctgattac tttaatttca ttaacagcaa cgtcaacagc ttttgcaata ccctttttta
      301 caatcattgg atttgcacct gctgctacat tcttcatacc ttctcttact atagcctgtg
      361 caagtaaagt agctgtagtt gtaccatctc ctgcaacatc attagtcttt gttgctactt
      421 ctttaacaag ctgtgcaccc atattttcaa atttatcttc aagttcaatt tctttggcaa
      481 tagtaacacc atcattggtt attgcaggag aaccgaattt tttgtccaat actacatttc
      541 ttccttttgg gccgagagtt attttaacag tatcagttaa ctgatttatt cctctctcta
      601 aagcgcgcct ggcttcttct ccgaataaaa tatctttagc cattatttta aacctcctgg
      661 taactaattt ttaagtacta acgcatctaa aagttattaa tctacaactg cgagtatgtc
      721 actttgtctt aaaatagtat actcttgatt gtcaactttt acttcagttc ctgaatactt
      781 gctgatgata actctgtcac caacttttac ttccattttt acttccttgc catctaccag
      841 gccacctggt cctacagcaa caatttcagc aatctgtggt ttttctttag ctgaacctgg
      901 taatacaata ccactctttg tagtttcttg agtttctacc atttttatta ctactctgtc
      961 acctaagggt tttattatca taatattacc tcctgtctca tttttccgtt ttgttagcac
     1021 tcaccttcat cgagtgctaa ttacaatact aataataata tattaacctt ttttaaatca
     1081 aatatgtata ttccttaaaa aatgaattta ttaccgtttt tcgccatact tattaggttt
     1141 taatctattt ttcactgtat tgcttctttt acccttatac tattgattcc actgcagaaa
     1201 gtcatgttcc aacaaaattt caattacatc gggaagatgc tgcgagggga aatgtctcta
     1261 aagcagttct tgcagcactt tccaaagtat tttgacccgg ttatctgcgt ttctttagtt
     1321 cactatatat atcttccggt tttaatccct gttctaccat caacacaaaa agatgatata
     1381 taagatcgga aatttcataa cgcagctctt caaaagagtt gtttttagcg gcaataataa
     1441 cttccgaagc ttcctctcca actttcttaa gtattttatc caaccccttg tcaaataaat
     1501 aatttgtata ggagccttcc ttggggttta tttttctatc ttgtataact tcatagagtt
     1561 cctgtaaaat atcaggccca acaatattat ccatatatat ccttctcctt tttatcttca
     1621 gccatatcta taaaaaacta tagctgcagt atttaaacct gctatccaaa tctgtcgttc
     1681 aaataagata ctatcatcaa ttctatatat aatccgtgct ttattataac tcaatagttc
     1741 taaaaaaaca gcttctatgc cccgtatgac aagcagcacc cttctgatct acttttagaa
     1801 gcagtgtatc tccgtcacaa tcaatattaa tagattttac atattggtaa tggccagatg
     1861 tctcaccctt gagccaaagt ttttttctac ttctgctcca ataatgagcc atacctgttt
     1921 gaagagtttt tgtaaaagat tcttcattca tatatgctag catcaataca tcatttgaat
     1981 atatatcctg gactatggcg ggaataaggc catccctatt gaatattagt tcagatatcc
     2041 tgcaatcaaa tttcagctta ttatctgagt tgcaatccat tttacggttc agcaccctat
     2101 ccagatcata actaacttta caatctgatt tacagtctgc tttacagtct gatttacagt
     2161 ctgatttacg gtctgattta cggtctgatt tacaatccga cttctgttta aattcgtatt
     2221 caggcatgca gcttaccctg ccttcttctc caatcacttt acaattactc ctttttcttt
     2281 taagtatttc ttcaactcca taattttgat ttcgccaaaa tggaacaaag aagctgcaag
     2341 tgctgcatcg gccctaccct ccgtaagcac acggtagaag tgctccatgg tacctgctcc
     2401 tcctgaagct acaacaggta tatttacact ttcagaaact gctcttgtta attcaatgtc
     2461 atagccgttc tttgtaccat ccatatcttt gcttgtaagg agtatttcac cagctccaag
     2521 tttttctacc tcttttgccc attgtatcgc gtcttgccct gaattggtat tcccaccgtc
     2581 tatatatact tcccagccag tatgatcagc cttttctgct gcatctattg caataataac
     2641 actattcctg ccaaatcgtt caactgcttc agatattaat tgaggtgttt taacggcagc
     2701 agtatttatg gatattttat ttgctccggc ttttaatact ctctcaatat cttctatact
     2761 tccaatacct ccacctacaa taaatggaat atccacctgt gctgcaactt tgctcacaat
     2821 gtccagcaaa gtcattcttc cctcaatagt ggctgctata tcaagaagaa ctaattcatc
     2881 tgctcctgct tcattgtatg catgtgcaac ttccacagga tcaccggcat cttttatatt
     2941 gctaaaattt acacctttta ctactctgcc atctttcata tcgaggcatg ggattattct
     3001 tttcccttca atcattaaac tatatcccct tctttactgt atggttcatt tcattattta
     3061 acatcacagc atatagcttg tgaacaaaaa agcacacgat tcgataaatc ttggaaggct
     3121 tattctgctg ctttcagagc ctcttttaga tccatatctc ctgtatataa agcttttcct
     3181 actattgctc ctgacactcc tatttcctta agtttcttta tatcatcaat gctgctaata
     3241 ccacctgatg ctataacttc catattaaca gcttctgcca tttctgcagt agccttaata
     3301 tttggacctg aaagcattcc atcttttgaa atgtccgtat atataattgt acgtgcaccc
     3361 agctcctcca ttttctttgc aaaaccaatt gctgtaaatt cactggtctt tgcccacccc
     3421 tctatagcta ccatcccgtc ttttgcatct atggctatag ctacattttc gctgaagagt
     3481 ttcagtgctt cctttacaaa atccggattt tcaactgcag aagtcccaag aatcactctc
     3541 tggatacctt tgctaagaag aatctctata gtgtctaatg tccttatgcc cccgcccaat
     3601 tgcacaggta ttcccagttt tgctgccatt tctttagcaa ccgtcgtgtt ttgaggctgt
     3661 cctgttctgg caccatcaag atcaataaca tgcaggtatc ttgcacccaa ttgctcccac
     3721 tttaaagcaa tttcaacagg gttatcggaa taaaccgttt ctttatcaaa ctggccttga
     3781 ataaggcgca cacatctccc tgctataata tcgatagctg gataaattat catatctcca
     3841 cccccatctt tttattttgt ataccttata ataacgcaaa aatgattcta ctaggtagaa
     3901 atagcaaact cgctttgccg gaggttaaaa tcctcaaatt ttattccctg taattctttc
     3961 ataagcctct atatatttgg cttcggtatt tttaactaca tcctctggaa gttcgggcgc
     4021 tggcggctgt ttgttccaat tgattgattc cagatattct cttacatact gtttatcaaa
     4081 acttttctgc ggtcttccgg gtgcgtaatc ttccatagtc cagaatcttg aagaatcagg
     4141 tgtgaacatt tcatctgcca ctataaggtt cccatcaagg aagccaaatt caagctttgt
     4201 atctgcaagg atgaggcctt tgctttccgc atatttactc gctttaaggt aaagggaaat
     4261 agttatatct cttaatttat ttgcaagttc aaatccaatt gtgtttgcca gctcttcaaa
     4321 tgttacactt tcatcatgac cttcatcggc tttagtagtg ggagtaaaaa ttggttcggg
     4381 aagcttctcg cattgtctca aatccgatgg tagctttatg ccgcaaatgg caccggtttt
     4441 ttgatattcc ttcaaccctg aaccttcaag gtaccctcta acaatacact ctgcctgaac
     4501 catttcagct ttcttaacaa gcattgcacg gccttccagt tcttctttaa atcttgagag
     4561 atcccccgga aactcatcga ctttggtagt taccatatgg ttgcctataa tatcttttgt
     4621 atagtcaaac caaaactcag aaatgctgtt caataccttg cccttgtttg gtataagtgt
     4681 tgggaaaata acatcaaagg ctgatattct atctgttaca ataataagta aattattgtc
     4741 aaattcatat acatcccgta cctttccctt cttaaataag ggtagtttaa taaaattagt
     4801 atcatttata agtttactca taaaaccctc ctatattttg tcataaattt ctctgggaat
     4861 acattcccgt tgcaataaaa atctgcaatg ctctctatac ataataatcc ctataataat
     4921 ctctatctat aatattccct tggtggacat tacccctttt attctttcat cctgctttgt
     4981 cgcttcatca agagctcttc caaatgcttt gaaaatagct tctgtcttat gatgatcatt
     5041 tttaccatat agaaccttaa tatgtaatgt tatacctgag ttaaaggctg cagctctgaa
     5101 aaactcttca atcatttgtg tattaaggtt ccctattttt tcggaagtga aacctccatc
     5161 ataaacaaga aacggtcttc cgcttaagtc cagagaaacc atagcaagag cctcatccat
     5221 gggaacatat gaaaggccat atcttcttat cattttttta tcacccaggg cttcttttat
     5281 tgcctgtcca agaacaatgc ctgcatcttc aacagtatgg tggcaatcca cgttaagatc
     5341 gccttctgcc tgtatctgca agtcaaataa accatgtttg gcaaaaaggt caagcatatg
     5401 gtcaaaaaaa cctattccgg tatttattga acttttaccc tctccatcaa tattaatgct
     5461 tattttaata ttcgtttcat ttgtttttcg ttcaatataa gccttcctat ccatttcata
     5521 cctccctgca gatgaattgt aaatttttta aatataacga ggctatattt gtcccccacc
     5581 tctataggtg gcgggtatcg ctagcattac catctaataa gatattatac caatcatcat
     5641 tataccagac aattataata taaaaaataa atttccacag atggatgcat tttaaattta
     5701 taaagaaata tattccagaa gctatcttta cggtgcttat aacacaagtc cctgaattat
     5761 tgtcaggaaa acttccattt cctcttctga acctatgctc actctcaaga aattatcaat
     5821 tccaggcttt ttaaaatatc tcaccaacac acctttttcc tttaaactta tgaaaagcca
     5881 ttctgcttct ttatcaggat gtgttataaa tataaagtta gcttttgagt caactactct
     5941 aaagcccatg ttttttagtg cctctgttac cctttctctc gtagaaataa tacgcctttt
     6001 tgttgtctga aagtaatcct catcttttat ggcctctatg cctgctttaa tagccaatct
     6061 gtcaatagta taagagttta tggagttttt gatcatgttt agagcatcaa ttaaattttc
     6121 attgcccaga gcaaatccaa gtcttaaacc cgctagagaa cgtgactttg aaaaagtctg
     6181 gattatgaga aggtttggat actcatcaat tagacctata actgaatctc ccccaaagtc
     6241 aatataagct tcatcaacta ttaccacatt atcacagttg tgttccaaaa tatttctaat
     6301 tgcttcaact ggaagatatt tccctgtagg tgcattagga tttgggaata tgataccccc
     6361 attctttgca aaaaacctat ctattggtat agataattcc ttatctaaag gtataagttt
     6421 gtaatcaata ttaaacatgc tgcaatatac tggataaaaa ctgtatgtta tatccgggaa
     6481 aagtattgga tctccagggt tgaaaaatgc cataaaagaa aatgcaagga gttcatcaga
     6541 accattccct gggaaaacct gcttttcatt aagcttataa taacttgaaa tagccttgcg
     6601 caattctata caatccggat ccggatataa tcttagagat tcatccgctg atttgattat
     6661 agcatcaaga acctttggag atggagggta tgggttctca tttgtattta gtttaatata
     6721 ttttttatct ttaggctgct cgcccggaac atatggttcc agtaacttaa ccctttcgct
     6781 ccagtattta ctcatatatt tctcctttca tgctatgggg acggttttcc ttttatatcc
     6841 tcgtgaggga cagttctcat cttgttattg gtgacattat cggggacagt tcttctctta
     6901 ttataaattt acatatttat agtctttgta gagaaccgtc cccattagtc gatagtcgat
     6961 agtcgagaac cgtctccatt agtcgatagt cgatagtcga gaaccgtctc ccttagtcga
     7021 tagtcgatag tcgagaaccg tctccattag tcgatagtcg atagtcgaga accgtctccc
     7081 ttattttata acccaggttt aactttctta ctcaaacctg acttttattg catttgcgtg
     7141 ggcacttaat ccttcagctt cagcaaaccg tactatatca tccttagcat caagtaaggc
     7201 tttttcagtg tacgaaataa tgcttgtttt cttgataaaa tctcctgcat ttaatggcga
     7261 aaaaaacctt gcagttccac ccgtagggag aacatgactt gggcctgcat aatagtctcc
     7321 aaccggctct gaagaaaagg aacccaggaa tatggctcct gcatttctta cagaacctaa
     7381 tatggagaaa ggctctttta tatataattc aagatgctca ggtgcaatct tattaacaat
     7441 ttcaactgca gcttcaatat tttcaactat tatcgcggct ccatattcct ccagggattt
     7501 tttaattata tcattcctgt ccaagtaccg atattgcctt tcaatttcct cttttacctg
     7561 accggccaac ttctcagaag tagttaatag aatagatgat gccataatat catgttcagc
     7621 ttgagataat agatcagctg caacgtattt gggattggca ttctcgtctg ctaccacagc
     7681 aatttcgctg ggtcctgcaa acatatcaat gtcacaatat ccatatacca ttctttttgc
     7741 agtggctaca taaatatttc caggtcctgc aattttatcg gctttaggta tggtctcagt
     7801 cccgaaagcc agggctgcta tcgcttgagc gccacctata cggtatattt catccacacc
     7861 agtctccttg gcagctgcca ggattgcagg gtttattttt ccgtctctgc ccggaggtgt
     7921 aaccataacc actctatcaa ctcccgcaac ttttgcagga attaaattca ttagcactga
     7981 tgaaacaagg ggtgcagttc ctccaggcac atacacgcct acaacctcaa gaggcctgta
     8041 caactgtccc agcaatactc catttttttc agttgatatc caggagtttt cgagctgctt
     8101 ttgatgatat ttttctatat tatcttttgc tctccgtata acagccagaa gttccgaatt
     8161 aacaacctta tatgcttcat ctatttcact gctgccaact tttaagtcct cagcttcaat
     8221 atccactcca tcaaattttt ttgtatattc tatgacagcg ctgtcccctt tagtttttac
     8281 attttcgagt atctcagcta ctacccggtt cacagtgtct ttacccttga aattcctttc
     8341 ggcaagctta ttgatgaaag cttctacttc tttcccacct gctttaaata tcccgatcat
     8401 agttttcacc ccatcgtttc ttttcagttt ttattccccg attctatctc acacgattct
     8461 atttcatttg tttttctatt tttcaggtgc atattttatt tttgaatgcg ctcctatttt
     8521 tctatctctt ttcttaacct gtcgataatc catgtaatct tttcactttt cattttcatg
     8581 cttactctat tcacaaccaa tcttgcacta atatcttcta tagtatcgaa aataaccaga
     8641 ttattctcct ttaaggttct tccactttcc accagatcca caataagttc ggccaaaccc
     8701 actaaagggg ctagttctac agagccatta agctttataa tttcaattga ttcctttctt
     8761 ctatgttcaa aatattctct ggctatcctt ggatattttg ttgcaaccct tttatttgtc
     8821 aacctgttca atttatcctg aagctccgca ggcccggcta acaccattct acatgctcca
     8881 aattttaaat taagcatttc ataaaggttt ctaccttctt caagcagcgt atctcttccg
     8941 gcaataccta tatcagcagc accatattcc acatatgtag gtacatcact tggttttaca
     9001 agaaaaaatt taatcttgtt attttcatcg gtaaaaatca attttcttga accgcttttg
     9061 atttcagtac agtctattcc tatggattca aacagctcaa tagcagtatc tccaagtctt
     9121 cccttagcca gtgcaatatt caaataattc atataatcct ccattacgct gctgctacca
     9181 gcagcacaaa tagtaacaaa atttccttcg tgctaaccat tctccaagca ccaaatatta
     9241 aatactctat gacactgcca ttcctctagc ttctgctgat cagttcactt actgtaacat
     9301 gctgcatctc gcctgtttca agattataga tctcaataat ttcactattc actaccctta
     9361 tgataccacc aatattcata gcttttgcgt atgaactgat ataatccatg cctgccattg
     9421 taatatccat ttctatgaca agtccttgtt ttctcatctc ctcacaaatt acaaaagctg
     9481 ttttcctgcc ctctttttca taacagatca aactatctat ttcctgcttt tttacatttg
     9541 ttttttgtct ttcaagagca ttcataacaa gatttactcc tatggaaaaa cctgtagccg
     9601 gtcgccctgc cccaaatttt tccaccaaag tgtcatatct tcctccgctt aaaattggaa
     9661 agcctactcc atgagtaaat cccctaaata ttatacctgt ataataattt aaactctgga
     9721 ccattcccaa atcaataaaa acatacttgc cgagtccatg atcttccaat atgcctagta
     9781 tgtttctaag ataatttaga gcatctaaag agcgcttttc tttaacaatt ttttctactc
     9841 tgtctataac atcaatagtc ccgaaaagac gtggaagatc aagaatcaat tcttttagtt
     9901 cctcagcaat attacaatga ttaattattt cctctattcc tataaaatcc tttcggtcta
     9961 ttagtattct catctgttcc acatcctgtt caggaaggcc tgtttcttcc attaacccct
    10021 taaaaaattc cacctggcct atatctatct gaaaattctc cattccggca gccttaagtg
    10081 cattaatagc tgttgcaatt acctctgcat ctgcttcagg tgttgcagag ccaatcaatt
    10141 caacacccgc ctgggtaaat tcattttgcc gtcctcctcc tgattcatta tatctatagg
    10201 tattccccac atagaaaatc ttcaggaggt attttgcaac cttaaatttg ctggctgcta
    10261 cccgtgcaac aggaatggta aggtcaggtc ttaaaaccag tattcgcccc tgatgatcaa
    10321 aaaacttgaa catcatttct tggggagtag agtcttcatc tactgaaaag gtatcaaaaa
    10381 attccagagt aggagtttca atttccatat acccgctttt tctaaatacc tctctgatat
    10441 tggattcaat attcctttta atatagcact cgtcaattaa tatatcctgt acaccttccg
    10501 gtgtataaat tttccactta ggcaataata acacctcaac tattcaattc aattggtgct
    10561 ttaccgtgat aaagttgtaa agttatacat cggtaaatta aatatattat aatgcatggc
    10621 aaatatg
//
LOCUS       DAOSDD010000155        10485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2412_length_10485_cov_5.180825, whole genome shotgun sequence.
ACCESSION   DAOSDD010000155 DAOSDD010000000
VERSION     DAOSDD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2412_length_10485_cov_5.180825"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..874
                     /locus_tag="PK733_13040"
     CDS             110..874
                     /locus_tag="PK733_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ11500.1"
                     /translation="MIKMVVTDLDETLLKSDKTISKYTIDIINKVKNQGIKFIFATAR
                     GGSAGNLIPSELFDGYVLMNGAKAYIDNRIIYDKTIPANVYSIFLRNLSRHSLKVAAE
                     INGVHYANFNVKEKWNYIDNFVITDYMDVSGGADKLYAIIENLNQVDLINSILPDELY
                     LNLSRDNLAMIMHKEAKKINGVLQIAREFNISKNEIIAFGDDTNDKEMLLGCGLSVAM
                     GNAIEDIKISTDYICDINDNDGVAKWLDENILWRNL"
     gene            1090..1419
                     /locus_tag="PK733_13045"
     CDS             1090..1419
                     /locus_tag="PK733_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11501.1"
                     /translation="MPFKKINVKDEINKRLENNIELKQAYDNAQHEYEVVKQIVKMRN
                     EMGLSQSDVARQSGLTQQMVSRIETVDNSPTLRNFIRYVDSLGLEIKIEKKNSETGRY
                     CAPYESQ"
     gene            1813..2166
                     /locus_tag="PK733_13050"
     CDS             1813..2166
                     /locus_tag="PK733_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ11502.1"
                     /translation="MQKLFDSEFKVMEIIWENEPISAKQVSLVAAEQIGWNKNTTYTV
                     IKKLEGKGYIKREEPGFICSSLISREDVCKTEAQSLIDKLFGGSKSALFSALLEDEKL
                     SSADLDELRKMIEKG"
     gene            2166..3083
                     /locus_tag="PK733_13055"
     CDS             2166..3083
                     /locus_tag="PK733_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ11503.1"
                     /translation="MALLSEVFYWLFNMSISASVVGIIILLLGKIKKLPRRLIHILWA
                     IPFLRMWIPIGMSSKYSLMTLISKFTTRTVVVYDGALDFTFTNHIMAADNYSPITYKV
                     NLLDNLFRAASMVWIIVAVALMIISVVLYVITKSELKDANRLRDNIYVSDRITAPATY
                     GVFRPRIIIPKGYELRDLQYILAHESAHINRKDNLWRILAIISVSVHWFNPFVWLFLK
                     RFLEETELACDERVLADCGEDVKKAYAIALIDYAESRNVFVSAFGGAKIRVRIDHILL
                     YSKLSIISIISFTVFAIAIGYVLLTNASL"
     gene            3096..4058
                     /locus_tag="PK733_13060"
     CDS             3096..4058
                     /locus_tag="PK733_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11504.1"
                     /translation="MTNFKKFYLFSVIGVLMASFYPLFMGVKVVADMVRNGTVLAENY
                     PKYIIPYTPISLAVIIAVLLLPVLMKYAQKFALVTASAISLVVFFASELLLESLVIVT
                     TTVTTTLESWQMFMCYVPPEGYQTRMWRAVDVLIGDYSPAFKIHFYIISVVLIFALLN
                     CFYGFAQMIITKNKSRLKALVIQSVFTALFLGLCIFACFTAFFRDGEITLSALSAVLM
                     SLFFVVFGVTAGVYVGSFLIEKKKTLSVLLPSIVASVVTLIMYIGEMILLSGHLYRFG
                     SGFLFDGLAGIVLAPIDILIIIASGCINAAICYTFSKPLKRLEI"
     gene            4503..4712
                     /locus_tag="PK733_13065"
     CDS             4503..4712
                     /locus_tag="PK733_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11505.1"
                     /translation="MSAAKIIRQVLTERDMSVKELAQALDITPQNMSNKLFRDSFSYK
                     EVVKIADVLECDVKVITRDTKKEFS"
     gene            4729..4857
                     /locus_tag="PK733_13070"
     CDS             4729..4857
                     /locus_tag="PK733_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11506.1"
                     /translation="MKIAYSKVAVKAINSLDDAMKQRIKIGIEGLIDSPPKGDIYK"
     gene            4871..5083
                     /locus_tag="PK733_13075"
     CDS             4871..5083
                     /locus_tag="PK733_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11507.1"
                     /translation="MSAISKQIIDMIDMLPEQEQQLAFEFIKRMVLAWDSDFTKLTPY
                     ERERLNNADKEIINGEIIDHSNIDWS"
     gene            5384..5650
                     /locus_tag="PK733_13080"
     CDS             5384..5650
                     /locus_tag="PK733_13080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ11508.1"
                     /translation="MGNRILEFGFNDLGLHRIIVHCYTENYGSYRVIERIDMRREGCF
                     LEARPANKFQIKSMAMNTHTLFLEMNGKSYRKSLIIIHYRLFLK"
     gene            5946..6209
                     /locus_tag="PK733_13085"
     CDS             5946..6209
                     /locus_tag="PK733_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOJ11509.1"
                     /translation="MAQTMVNFRMDEDLKKSMEKICSDMGLSMTAAFTIFAKKVSRER
                     RIPFEVSADPFYSENNMKHLEQVIADIAAGRAKLVEHDLTEVD"
     gene            6434..8950
                     /locus_tag="PK733_13090"
     CDS             6434..8950
                     /locus_tag="PK733_13090"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ11510.1"
                     /translation="MSYYPHLNDIFITKRLRNRLNEIGCHTFTTVIAPMGFGKTTAVR
                     WWAKHLPKSNPDAVILRQIIATDSTTDFWNGFCRAFKDYPELNEQMKVLGYPKDAGAV
                     SVLAELLSDALHNSKSPLYFILDDLHILNQKSLTPMLLFLSRSLPDCVHMILLSRNQI
                     FSEEERMRLGNLLCEIGMEDLRLTEQEVVLYARRCRLEALDDKLKTLAELSEGWISMI
                     YLNFKSYAQNGRWLSSSSDIFTLIDQVLLEPLPQRQKEFLILIGMTDEFTEPQAAYLW
                     QHEDAVILLNSLSKNNAFITKSENGIYRYHHMLRQCVRQKFTEKPEKYQKESYDRLGK
                     WHFKQREYIPAYFAFAKAERWSALLEVLEKDKAKSLNTEHSQGFFNWIQTCPEDLLRR
                     HPLAIVVCMVKMFSFHNIPEIKRMKRLLLEALGQDTALTPQQRDDLLGDAELSESFLC
                     YNDISAMSAYHRRACALLSRTSLSVDPKGAWTFSAPSVFMMYHREAGKADNENAEMKE
                     CMPYYYQVSDGHGNGAEHTFAADLFYERGQLTDADIANRMAMSAARRKEQFSIMLCSE
                     FLSMRMELFQGNYESMNKRICDSREWLHHGRQYTLLNTLDMCQGFIYALLGQPDAAPE
                     WFLKGRLDEALVMFPATPMLHTFYNQLLLTKGEWTQVLARQEECEKLYGVYHNVLCEI
                     WLHIQLSAAYEQLGRRKQAQAELITALNMALPDGILMPFAESERYLLDVLKDIQNASV
                     HSQEIDLILPLAQRFGDSRRSICREHFGEYETYGLSDRELAIARLAAQRKTNAEIAKE
                     LHIAEGTVRNQLSRVFDKLNLSGEGKNKRLALEEVLKIKK"
     gene            9097..9354
                     /locus_tag="PK733_13095"
     CDS             9097..9354
                     /locus_tag="PK733_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11511.1"
                     /translation="MKGKPITMVEPQDGGILRVIFDTGNMVTVDMKPSFAGFRFGALQ
                     SREVWSTADTDGSFVHWYKNGIPVAELAYNEIMKMTLGESY"
     gene            9369..>10485
                     /locus_tag="PK733_13100"
     CDS             9369..>10485
                     /locus_tag="PK733_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11512.1"
                     /translation="MKSKNGLRWKQIGSLFMAVCMVLTMLPITAFAETGARDPGTPLG
                     ASGIITAFSELDTEVSEQTVQTGTPEDELNLPSALADTVAESVYAINNSALPIPGDVL
                     QMESGVLKDGVLVFDLGDYDTFKASLTNGERFPITINRSKGGEAGYPVTAKLDSYYGS
                     NDICFKIFDFIEGDDCNDGLIEFPPNVTSKTIYIGEEYEYGDNTIRSGTLSSYIYLYD
                     FDKTTLATPMIRLETTKAKSTGVDTAGLPPVAYAQVGSATPSVKPGESKVISVTFFPS
                     ANIFQQDRGFCEITGDLTLNLKKGTETQALKPVEAVGSILSDVTFVIPPINLVDKEWE
                     VVSITGIKNAANSDVIELTYSDDYGESDISVSSYTFWY"
BASE COUNT         3305 a         1799 c         2376 g         3005 t
ORIGIN      
        1 gaaaacgctc cgacttctgc aaaacagata gctaacctgc gtgaagcggt tggctagaac
       61 cgaatggagg gcaattataa aaattctttt ataactgagg ggggatgtta tgattaaaat
      121 ggttgtaacg gatttggatg aaacccttct taaatctgat aaaactattt cgaaatatac
      181 aattgatata ataaataagg tgaaaaacca aggaataaaa tttatatttg caacagccag
      241 aggcggtagt gctggtaact taataccatc tgagctgttt gatggatatg tgctaatgaa
      301 cggtgctaaa gcttatatcg ataatagaat tatttatgat aaaactattc ctgcaaatgt
      361 ttatagtata tttttacgga atttatcaag acatagtcta aaggttgctg cagagattaa
      421 tggggtgcat tatgctaatt ttaatgtaaa ggagaaatgg aactatatag ataattttgt
      481 tataactgat tatatggatg tctcaggcgg tgctgataaa ttatatgcca ttatagaaaa
      541 tctaaatcag gtagatttaa ttaattcaat ccttccggat gaattatatc ttaatttaag
      601 cagggacaac ttagctatga taatgcataa agaggctaaa aagattaatg gagttttgca
      661 aattgctagg gagtttaaca tatccaagaa cgaaataata gctttcggtg atgataccaa
      721 cgataaggaa atgctacttg gttgtggttt gagtgtggct atgggtaatg ccatagaaga
      781 tattaaaatt agtacagatt atatttgtga tataaatgat aatgatggag tagctaaatg
      841 gctagatgag aatattttat ggagaaattt atagcttagt aatatatgaa tctaggaaca
      901 agtaagaaag aacaaagaag ataattatta aattaatgaa aaagatatgt tagagagatt
      961 ataagaaatt agggaactaa atgagaaagc ggaaggatta acaatcctga ataaacttga
     1021 agatgaagga acttgaaaaa gagcttggta gaaggtttct ataagtagtg gaatgggagg
     1081 tgaacgtgta tgccgtttaa aaaaatcaat gtaaaagatg aaatcaataa gagacttgaa
     1141 aataatattg agcttaaaca ggcttatgat aacgctcagc atgagtatga agttgttaaa
     1201 cagattgtga agatgagaaa tgaaatgggt ttatcacaga gtgatgttgc cagacaatca
     1261 ggattaacac agcaaatggt ttcaaggatc gaaaccgtag ataattcacc tactctaagg
     1321 aattttatcc gatatgtaga cagtttaggg ttggaaataa agattgaaaa gaaaaactct
     1381 gaaacaggta gatattgtgc tccctacgaa agtcagtgac aaataataaa agctgtgaaa
     1441 agtttgatac tattttgata caaaaaatct tgcaagtcct aaataatcat aataaccagg
     1501 ataaaaaagc aaacataaag cataaatata ttaacaaata tgttaaatta aaagaatgtt
     1561 ggattgagtg ggaatgattt gaaggacggc acaaagccag taatcgcacg gttttgctgg
     1621 ctttcaaaca ctcctgtagc aactactgaa agattgcata tttctaagca aacaaaagca
     1681 gtgggataag gtattgaact tatccactgc ttttgtgact tttttctgat atttccttaa
     1741 cttctattga cagcatcaga ctattgagat agtatagact ataggaatag tcttgcggag
     1801 gtgtaggttg taatgcaaaa attgtttgac agcgaattta aagttatgga aatcatatgg
     1861 gaaaatgaac caatcagtgc caagcaggtc agtctggttg ctgcggaaca aatcggttgg
     1921 aacaaaaata ccacatacac tgttattaaa aaacttgaag gcaagggata tatcaaaaga
     1981 gaagaaccgg ggtttatctg ttcctcactg atttcccgtg aggatgtttg caagaccgaa
     2041 gcccaaagcc ttatcgataa gcttttcggt ggttcaaaaa gtgcactttt ctctgcttta
     2101 ctcgaggatg aaaaactctc ttccgctgat ttggatgaat tgcgaaagat gattgaaaag
     2161 gggtgatggc attgctttcc gaagtattct attggctttt caatatgagc atatctgcat
     2221 ctgttgttgg catcatcatt ttactattag ggaagattaa aaagcttcca cggcgcctga
     2281 ttcatatatt gtgggcaatt ccttttctta gaatgtggat tcctatcgga atgtcaagca
     2341 aatacagtct gatgacactg atttcaaaat ttactacaag gactgtggtt gtatatgatg
     2401 gtgcattgga ttttaccttt acaaatcata ttatggcggc agacaattat tctccgatta
     2461 cctataaagt gaatctgctt gataatttat tccgtgctgc ctctatggta tggattattg
     2521 ttgctgttgc tcttatgatt atttctgtag tcctttatgt tattacaaaa tctgaattaa
     2581 aagatgccaa ccgtttacga gataatattt atgtttcaga cagaataacc gcaccggcaa
     2641 catacggtgt ttttcgtccg agaattatta ttcccaaagg atatgagcta cgtgacttgc
     2701 aatacattct ggcacacgaa tcggcgcata ttaatcgtaa ggacaattta tggcgtatcc
     2761 ttgctattat ttccgtctct gttcactggt tcaatccgtt tgtttggcta ttcttaaaga
     2821 gatttttgga ggaaaccgaa cttgcctgtg atgagcgagt attggcagac tgtggtgaag
     2881 atgtgaaaaa ggcttatgcg atagctctta ttgactatgc tgaaagcagg aatgtttttg
     2941 tctcggcatt cggtggggca aagatacggg tacgtattga ccacattctg ttatatagta
     3001 aactgtcaat catttcaatt attagcttca cagtatttgc cattgcgatt gggtatgtgc
     3061 ttctgacaaa tgcttcttta taaggaggga gatcaatgac caattttaag aagttttatc
     3121 tattctctgt gattggcgta cttatggcat cgttttatcc cctgtttatg ggtgtgaaag
     3181 ttgttgccga tatggtacgg aatggaacgg ttttagcaga gaattatccg aaatacataa
     3241 ttccatatac gccgatcagt ttggcagtca taattgcggt tttgctttta ccggtcttga
     3301 tgaaatatgc ccaaaaattc gcattggtaa cagcatccgc aatatctctc gtcgtctttt
     3361 ttgcatcgga actgctgctt gaaagtttgg tcattgttac aacgactgtt acgacaacac
     3421 ttgaaagttg gcaaatgttt atgtgttatg tgccgccgga aggctatcag acaaggatgt
     3481 ggcgggcagt tgatgtgctg atcggagatt acagtccggc atttaaaatt catttttata
     3541 ttatttccgt ggttttgata tttgctctct taaactgctt ttatggcttt gcccaaatga
     3601 ttattaccaa aaacaaaagc aggctaaaag cactggtgat ccaatcggta ttcactgctt
     3661 tgtttcttgg tctttgcatt tttgcctgtt ttaccgcatt tttcagggat ggagaaatta
     3721 cattatctgc tctttcagcg gtgcttatga gtttattctt tgtcgtgttt ggcgtaacag
     3781 caggcgtata tgttggctca tttctgattg aaaaaaagaa aacgctttcg gtattgctgc
     3841 cgtcgatcgt ggcatcagtc gttacactca ttatgtatat cggtgagatg attctgctga
     3901 gcggtcacct gtaccgtttc ggctccggat ttttatttga tggactagca ggtattgtac
     3961 tagcccctat agatatatta attattatag catcaggatg tataaatgct gctatatgct
     4021 atactttcag caaaccatta aaacgccttg aaatttaagc acaaatcgga taaaatggaa
     4081 atccttgtga tgtttagtca aaaaacatgg caacatattg gcaacaaaaa atcagatagc
     4141 ttaaaatatt tggataattg atataaatag agtagcacag ctcacaaaac ttaaacagaa
     4201 ttgtttagac tatgtttttg gtgagcgagt gggaatagta aagaaaaacc cttaattctt
     4261 gtataatcaa ggattacagg gtttaatttt gttcaatcgt actgaaaaca tactattttt
     4321 taaaatcact tttcaacatt atcttcttat atgcccggtt gcatttcagt aacatcattt
     4381 ctaatgttaa gctgctgaag cttatctaca acttcagaaa ttataacaaa tcaggttata
     4441 ttttaattgc tattgaaata taatacacat atatgttata acaaaaacga ggagggatgt
     4501 gtatgtctgc agcgaagatt atcaggcagg ttttaactga acgagacatg agcgttaagg
     4561 agttagccca ggctcttgac attacaccac aaaatatgag caataagtta ttcagagata
     4621 gtttttcata caaagaagtt gtaaaaatag ctgatgtttt agaatgtgat gtaaaagtaa
     4681 ttacgagaga taccaagaaa gaattcagtt agaaaggagg aaataacctt gaaaattgca
     4741 tattccaagg tagctgttaa ggctataaat agtcttgacg atgctatgaa acaaagaata
     4801 aagattggaa tagagggatt aattgattca ccgcctaagg gagatattta taagtaaagg
     4861 agatgattag atgtccgcaa tatcaaagca gataatagat atgattgaca tgctgccgga
     4921 gcaggaacag cagttagcgt ttgaatttat aaagaggatg gtcctggcat gggacagtga
     4981 ttttactaaa ctgactcctt atgaaagaga aagattgaac aatgcagata aagaaattat
     5041 aaacggtgaa ataattgatc attccaatat tgactggagc tgaacaatat aatgtgtggt
     5101 tctattcagg ctttaaaata tttgaagaat aaaaataata tatataacat atgcatttaa
     5161 tatataaaga acgcataaaa gcacatagaa tcataccaaa acacctttct ataattgaat
     5221 ggacattctt tgtggtggaa atttcattga tcatggttat gtgggattat gaaaataaga
     5281 gcaatgagag atatatatcg atatgaatga ctatattatg actatgcgaa agttgatagg
     5341 aaatgaaatg aaatgctcat gacagttgga tggatgtact ataatgggca atcgaattct
     5401 agagtttggt tttaatgatt tgggacttca caggatcatt gtccactgtt atacagaaaa
     5461 ctatggttca taccgtgtta tagagcgtat tgatatgcga agagagggtt gctttcttga
     5521 agcacgacct gcaaataagt ttcagataaa aagtatggcg atgaatactc atacgctatt
     5581 cttagagatg aatggaaaat cgtacaggaa atcgcttatt ataattcact atcggttatt
     5641 tttaaagtaa aattaaatta tataaagatg tatttgaata aatatgtgtt ttttaaatag
     5701 aataggagga catctatttt taggcgtcaa aaatgatgga gtaataatag gtgttgaaga
     5761 aaaatcaata gaaaaaatat aatttgactt tattacatct atgaataatc caatgaaaat
     5821 taatcctaat atagtgtata aatagagagc aattacatat aatactgtaa agcgattgta
     5881 aagaaaacga ttgacatatg ctttacggta tgttatctaa aagcagatag ataggagtga
     5941 tatttatggc acaaacaatg gtaaattttc gcatggacga ggatttgaaa aagagcatgg
     6001 aaaagatttg ttctgatatg ggactttcta tgactgctgc ttttacgatt tttgcaaaaa
     6061 aggtttccag agagaggcgt ataccttttg aagtttccgc cgacccgttt tattctgaaa
     6121 acaatatgaa gcatttggag caagttatag cagatattgc cgcaggtcgt gcgaagttgg
     6181 tcgaacatga tttaacagag gtagactaat gcggctatta tgggaagaaa gagcatggga
     6241 tgaatattgt tggggtataa gatgaaatta tattttgtat ggtgtatcag taagcaattt
     6301 caaagtggat atctttccat tgagctaagt tttctcttga tcacttccga tgcaagaaat
     6361 ataatagata aaaagaatga aaatgggcgt aagcctttca tgagtaaaca aaagcggagg
     6421 aaggagatgc tttatgagtt attatcctca cttaaacgat atttttatta ccaagcggct
     6481 gaggaatcga ctgaatgaaa ttgggtgcca tacgttcacc acggttatcg ctcccatggg
     6541 ttttggcaaa actactgcag taagatggtg ggcaaagcat ttgccaaaaa gcaatccgga
     6601 tgctgtgatt ttacgccaga taattgctac agatagcacc acggactttt ggaacgggtt
     6661 ttgcagggct tttaaagact atccggaact taacgaacag atgaaggtgc tgggttaccc
     6721 taaggatgcc ggcgctgttt cagtgctggc agagcttctc agtgatgctt tacataacag
     6781 caaaagccct ctttacttca tattggatga tcttcatatc ctcaatcaaa aatctttaac
     6841 tccgatgctg ctctttctct cccgcagtct gccggactgt gtgcatatga ttcttttgtc
     6901 acgcaatcag attttcagcg aagaagagcg gatgcgcctc ggtaatctgc tctgtgaaat
     6961 cgggatggaa gatttgagac ttacggagca ggaggttgtc ctatacgccc ggcgatgccg
     7021 ccttgaggca ttggatgaca aacttaagac tctcgcagaa cttagcgagg gatggatctc
     7081 catgatctac ctgaacttta agtcctatgc gcagaacgga cgctggcttt ccagctcatc
     7141 tgatatattc acgctgattg atcaggttct tttagagcca ctgcctcaga ggcaaaaaga
     7201 gtttttgatt ctcatcggta tgacagatga gtttacagag ccccaagcag cctatttgtg
     7261 gcagcatgaa gatgcggtaa ttttattaaa ctctctttcc aaaaacaatg catttattac
     7321 aaaaagtgaa aacggcattt atcgctacca ccacatgctg cgccaatgcg tccgacagaa
     7381 gtttactgaa aaaccggaaa agtatcaaaa agagagctat gacaggcttg gaaagtggca
     7441 ttttaagcag cgggaatata tccctgctta tttcgctttt gcaaaggcgg aacgatggag
     7501 cgctcttttg gaagttttgg aaaaggataa ggcaaaaagc cttaatacag agcatagcca
     7561 aggatttttc aactggatac aaacatgccc cgaagacctt ttgcgaaggc atccccttgc
     7621 aattgtcgta tgtatggtca agatgttttc ctttcataat ataccagaaa tcaaacgcat
     7681 gaagagactg cttttggaag cgcttgggca agatacagcc ctaactccgc agcagcgtga
     7741 tgaccttttg ggagatgccg agctttcgga gagcttcctc tgctataacg acatctctgc
     7801 tatgagtgcc taccaccgca gagcctgtgc gcttttaagc cgtacctctc tcagcgtaga
     7861 tcccaaagga gcatggacct tttctgcccc ttctgtcttt atgatgtatc accgggaagc
     7921 aggcaaggca gataacgaaa acgcagaaat gaaagagtgc atgccctatt actatcaagt
     7981 atcggacgga catggaaatg gtgcggagca cacttttgcc gctgacttgt tttatgagcg
     8041 ggggcaactg accgatgctg atattgcaaa tcgaatggcg atgtcagcgg cgagacgaaa
     8101 agaacagttc agcatcatgc tgtgcagtga attcctctct atgcgaatgg agctgtttca
     8161 gggcaattat gaatcgatga acaaaaggat atgtgacagc cgagaatggc tgcatcatgg
     8221 gcggcaatat acactgctga atactctcga tatgtgccag ggctttattt atgctctgtt
     8281 agggcagcct gatgccgcac ccgaatggtt cttgaaggga cgacttgatg aggcactggt
     8341 gatgttcccc gcaacaccaa tgctgcacac cttttataac cagctactac ttaccaaagg
     8401 agaatggaca caggttttgg caaggcagga ggagtgtgaa aagctgtacg gtgtctacca
     8461 caatgtgctg tgcgaaatct ggctccatat tcagctatct gccgcctatg agcagcttgg
     8521 caggaggaag caggcgcaag cggaactgat tacagcgctt aatatggcac tgcccgatgg
     8581 cattctaatg ccctttgccg aaagcgagcg ttatcttctt gatgtgctga aggatataca
     8641 aaatgcgagc gtccattcgc aggagattga tctcatttta ccccttgccc agaggtttgg
     8701 tgattcaagg aggagtatat gccgggagca cttcggggaa tatgagacct atggactgtc
     8761 tgatcgggag ctagcaattg cgaggcttgc ggcacagcgc aaaaccaatg cggaaattgc
     8821 aaaagagctg cacattgctg agggaacagt ccggaatcag ctttcccgag ttttcgacaa
     8881 gctgaatcta tcaggagaag ggaaaaacaa acgccttgcg ctggaagaag tgctgaaaat
     8941 aaaaaagtag tgacaaatgg tcacactaca aaatggaaac gctattgtac aatacatttg
     9001 tacagtggcg tttttttatt agaggtgcat acctgcacct aaactcagca tgactcaatg
     9061 tcgtgctgag tttgatttaa atgaggaggt gaaagcatga aggggaagcc aattactatg
     9121 gtggagccgc aggacggcgg gattttgcgg gtaatctttg acacaggaaa tatggtaaca
     9181 gtggatatga agcccagctt tgcgggtttt cgatttggtg cactgcaaag ccgggaggtt
     9241 tggagcacgg cggacaccga cggcagcttc gttcactggt acaaaaacgg cataccagta
     9301 gcagaacttg cctataatga aattatgaag atgactcttg gggagtcata ctgaagggag
     9361 acaatagtgt gaaatcaaaa aacggtttaa gatggaagca aataggcagc ctgttcatgg
     9421 cagtgtgcat ggtactgact atgctgccca ttacggcttt tgccgaaaca ggcgccaggg
     9481 atcccggcac acctttgggt gcaagcggga ttatcaccgc cttttcagag ttggatacgg
     9541 aggtatctga gcagacggtg caaaccggca caccggagga tgaactgaat ctgccgagtg
     9601 cgctggcaga cactgtcgcc gaaagcgtct atgccataaa caattccgca ttgccgatac
     9661 ccggcgacgt tcttcaaatg gaaagcggag tgctgaagga tggtgtgctg gtcttcgatc
     9721 tgggggacta cgatacattt aaggcatctc tcacgaacgg cgaacgcttc cccatcacca
     9781 tcaacaggtc taagggcggc gaggccggat atccggttac cgcaaagtta gactcatact
     9841 acggcagtaa tgatatatgc ttcaagatat ttgacttcat agagggagat gattgcaacg
     9901 acggccttat cgaattccct cctaatgtga cgagtaaaac aatatacatc ggagaagagt
     9961 atgaatatgg cgacaatacc attcgcagcg gtacgctcag ctcctacatt tatctctatg
    10021 attttgacaa aaccacgctc gccaccccga tgatccggtt ggaaacgaca aaagcgaaga
    10081 gcaccggggt agatacggct ggcctgccgc ctgtcgcgta tgcccaagtg ggttctgcca
    10141 ccccatctgt gaaaccaggc gagagcaagg tcatcagtgt gacctttttt ccgtctgcta
    10201 atatctttca acaggataga ggattttgcg agattacagg cgatttgacc ctgaatctga
    10261 aaaaaggtac ggaaacacag gcactcaagc ccgttgaggc cgtcggatca atcctgagcg
    10321 atgtgacgtt tgtaatccct cccattaatc tggtcgacaa ggaatgggaa gttgtcagca
    10381 tcaccggcat aaagaatgcg gcgaacagcg acgtaataga acttacgtac agcgacgatt
    10441 atggcgagag cgacatttct gtctcatcgt atacattctg gtacg
//
LOCUS       DAOSDD010000156        10482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2413_length_10482_cov_4.620504, whole genome shotgun sequence.
ACCESSION   DAOSDD010000156 DAOSDD010000000
VERSION     DAOSDD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_10482_cov_4.620504"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            720..2000
                     /locus_tag="PK733_13105"
     CDS             720..2000
                     /locus_tag="PK733_13105"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOJ11513.1"
                     /translation="MARREIIAMILAGGQGSRLGVLTKKIAKPAVMYGGKYRIIDFTL
                     SNCVNSGIETVGILTQYQPLKLNSHIGIGKPWDLDRMHGGVTILSPYIKDEKGEWYKG
                     TANAIFQNIDYIDKLGPEYILILSGDHVYKMDYSKMLDFHKQNNAEATISVINVPIEE
                     ADRYGIMSIDDRGKIYKFEEKPKNPQSTLASMGVYIYTWEVLKNYLIKDNIDEQSEHD
                     FGKNIIPNMLKDGRSLWAYEFSGYWKDVGTIQAYWESNMELVKRLPEFNLYEQTWKIY
                     SFNPIMPPHYVDSKGSVNTSVVAEGCMIYGTVKNSVIFPGVVIEEGALIEDSIVMSKS
                     RVGKGAYINHSIIGEGVCVGSNVRIGVGEPVPNEYKPGVYNSGITVVGDNACIPGNVV
                     MGKNVMVDIGVGPEDFCSLDVPSGKSVFKGGDCE"
     gene            1997..3118
                     /gene="glgD"
                     /locus_tag="PK733_13110"
     CDS             1997..3118
                     /gene="glgD"
                     /locus_tag="PK733_13110"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOJ11514.1"
                     /translation="MKNTLGVVLTGGKYQGLKDLSAIRSGSAVPVGGKYRAIDFVLSN
                     MVNSGIKKVGVITQYSFRSLMDHLGSGKEWDLDRRYDGLFIFPPSLSDDDSGIYMGSA
                     DAMYKNLSFFKRSNEEYALLAQGNCVYKMLYKQMLEYHIEKNADITIAYREMDDYTPA
                     ELTRLGIISLDKDERIIDFQEKPLNPATLNGSIGIYILKRELLISLLQECAAHGNYDF
                     VKDILIKKLPELKIYGYKFSGYWRNLSSVRMYYRCNMELLDPGVRQELFINNGKIYTK
                     VKDETPAKYNAEAEVKNSIVADGCIIEGTVENSILFRGVIVGKGSVIRDSIIMQSTYI
                     GENVNIQHAILDKNVVINNEKQFRGDSDWPVIIGKDAMV"
     gene            3176..4252
                     /locus_tag="PK733_13115"
     CDS             3176..4252
                     /locus_tag="PK733_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ11515.1"
                     /translation="MKRFLKILTAIVLVVSIALSISLSGCGRGKKDKIEKSIDDNNVE
                     KAQPEPEIDLEKIRPDESGKIMVVMFHNFVESFTPTKYDKGEYTITFDGFEKLLHTLY
                     NNGYRLISFKDYLENNITVQAGCVPMVFTFDDGTSGQFNFIEENGELKVNRNSAVGIM
                     EDFNKLHPDFGLKGMFFVNLGLSTFNGKGELQQRLKYLIGKGFEIGNHTFNHINLKDA
                     KSSEKIQQELGKNQKKMYELVPGYKMYAFSLPYGAPSNDLMKYVIKGEFEGEKYENLA
                     IVEVGWDPALSPVNKKYDPVSTHRVRAPGINPEKFDLNWWLENISRKDEYVSDGNPKK
                     VTIPKEKSDVLDMEKLKDTELITY"
     gene            4464..4775
                     /locus_tag="PK733_13120"
     CDS             4464..4775
                     /locus_tag="PK733_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11516.1"
                     /translation="MANGKTKKVQTDFDVKRKAVKLVITHLKKKVSKEYMGREHINNW
                     IAEMEELMSKPDFDMVEYIDMRKKLNDVIERTLDEEMRYKIRDSWYSLGRALDKKVKH
                     R"
     gene            4997..5227
                     /locus_tag="PK733_13125"
     CDS             4997..5227
                     /locus_tag="PK733_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11517.1"
                     /translation="MLFNRNIRNIFNEVVNEKKRSKLTVEKMREELIRNLNKKGIRTS
                     IVQINMDSDNLMIRLHIDDNNAAFRSRIQWGS"
     gene            complement(5261..6634)
                     /gene="argH"
                     /locus_tag="PK733_13130"
     CDS             complement(5261..6634)
                     /gene="argH"
                     /locus_tag="PK733_13130"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOJ11518.1"
                     /translation="MKLWGGRFVKATDKSVNDFNSSIRFDSRMYRQDIIGSIAHVKML
                     GSCGIIPLEEAELIQKTLLDILADIEKGLIEFEIGAEDIHTNVEKLLISRIGDTGKKL
                     HTGRSRNDQVALDIRMYLKDEINSIKGMLISLENTLLSMAEKHLDTILPGYTHLQRAQ
                     PITFAHHMMAYFQMFRRDIERLGNCYERTDIMPLGSGALAGTTYPLDRHMVAEELGFN
                     AITENSLDGVSDRDFIIELLSCLSIVMTHLSRFSEELILWSSHEFRFIEMDDSFSTGS
                     SIMPQKKNPDVAELVRGKTGRVYGDLVAMLTTMKSLPLAYNKDMQEDKEAVFDAIDTV
                     KLCLPVFTNMIATMKVNTDYMYKAAQGGFTNATDVADYLVKKGVPFRDAHEITGRIVL
                     YCIDNSKTIDALSIDQLKTFSSLIQDDIYKEISLEKCVSGRNVPGGPSKESLLMSIKK
                     GYSFLKV"
     gene            complement(6819..8030)
                     /locus_tag="PK733_13135"
     CDS             complement(6819..8030)
                     /locus_tag="PK733_13135"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOJ11519.1"
                     /translation="MSKKEKIILAYSGGLDTSIIIPWLKENYDYEVIAMAADVGQGEE
                     LEPLREKAIKTGASKIYIENIQEEFVTDYIFPTLKAEAIYEGKYLLGTSFARPIIAKR
                     MVEIAEKEGATAIAHGATGKGNDQVRFELTVKALAPHLKIIAPWRIWDIKSREDAIDY
                     AQERGIPIPVTKKDNYSMDRNLWHLSHEGSDLEDPWNEPQYDKILKMIVPPEKAPDSA
                     SYVEIYFDKGIPVKVNGVEYSPIELIEVLNKIGGENGIGIMDMVENRLVGMKSRGVYE
                     TPGGTILYAAHRELELLCLDRDTLHYKDLIAQKFSELVYFGQWFTPLREALSAFVDST
                     QQTVTGTVRMKLYKGNCMPAGSKSPYSLYSEELSTFGRDNVYNQKDAEGFINLFGLPL
                     KVNALMNQKNK"
     gene            8406..8915
                     /gene="ruvC"
                     /locus_tag="PK733_13140"
     CDS             8406..8915
                     /gene="ruvC"
                     /locus_tag="PK733_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOJ11520.1"
                     /translation="MIIMGIDPGFAITGYGIIKYEGNRFTVLEYDAIITKPSEKFAER
                     LLTLNNRIKKLINIYRPEAFSIEELFFNKNVKTAITAAQGRGAAMVAAAECGISIYEY
                     TPLQVKQAVVGYGRAEKSQIQQMVKVILNLPEIPKPDDVADALAIAICHAHSYKMENI
                     AKCQEAKDK"
     gene            8912..9517
                     /gene="ruvA"
                     /locus_tag="PK733_13145"
     CDS             8912..9517
                     /gene="ruvA"
                     /locus_tag="PK733_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOJ11521.1"
                     /translation="MISYIKGTLEYKHTDYVIVEVHGIGYKVFTSLSTIQSIGQVGSQ
                     VKLHTYLQVREDNISLYGFATPEELKMLELLLKVSGIGPKVSISIMSNLSPSKFSLAV
                     ITDDVKTLTKVPGVGNKTAQRIILELKDKIRKEQGDISDFASTEDSSVVDRAGMKASE
                     AITALMVLGYTALEANRAVSAVYSEELDLETVIKNSLKKLI"
     gene            9589..>10482
                     /gene="ruvB"
                     /locus_tag="PK733_13150"
     CDS             9589..>10482
                     /gene="ruvB"
                     /locus_tag="PK733_13150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOJ11522.1"
                     /translation="MEERLVTCEAVGDDQDEASLRPRRLNEYIGQSRVKENLSIFIEA
                     TKQRKEALDHVLLYGPPGLGKTTLAGIIASELGVNLRITSGPAIEKPGDLAAILTNLG
                     DYDVLFIDEIHRLSRSVEEILYPAMEDYALDIIIGKGPSARSIRLDLPKFTLIGATTR
                     IGLLTSPLRDRFGVINRLEMYSAEELKLIVKRSAAILNIGIDEEGALEIAVRSRGTPR
                     ITNRILKRVRDFAQIKADGFINRDVARMALDALEVDNLGLDGIDREMLFCIIEKFDGG
                     PVGLDTLAAAINEEAGTIEDVY"
BASE COUNT         3466 a         1719 c         2124 g         3173 t
ORIGIN      
        1 cataaaatta aaactggcac agttttactt cattgattgc atttattata acataagtgc
       61 aatccgccaa tcggattgct tcacaccgtt tcaataaatg cataaaatta aaactggcac
      121 agttttactt cattgattgc atttattata acatattaag atatttaact gcaattaaat
      181 gcaccctacg aggtgcattt tctatttgaa gaaaaatatg gcaagaaacg atttccatgt
      241 ataatattca cttcagcaac ggattgtaga gtttaagtct acagaaaaat tctacccgtc
      301 acatacacca tccaggtgtc tcgtatgttg acagcttatg catacgagac acaacattaa
      361 tgttgtgcat tattaacaca ttaatagttt ggttgtgtta ataagcacat gttttggtga
      421 cggctcacga catctgtgtc gtgaattacg aatttttcct tgccttaaac ataacaatcc
      481 gtaagctttg ttcaaatttt atactcggaa aatcgtatgc ttccatgttt tgtattatag
      541 gaagaaaatg cgcctaccct accccatcta gcatatgttc ttaaaatatg aattgcccgg
      601 gaaatagggc ttgtgtttct ctgaactcaa aaaaatggtt tattggctaa aattatggta
      661 taattttatc atatagagaa tacaattctt gttgaaatca aatgaaaggg gaaaggtata
      721 tggcacgtag agaaatcatt gcaatgattc tagcaggtgg ccagggaagc agacttgggg
      781 ttttaaccaa gaaaattgcc aagccggctg tcatgtatgg aggcaagtac aggattattg
      841 attttaccct aagtaattgt gttaattcag gaatagaaac agtaggtata cttacgcaat
      901 atcaacctct taagcttaac tcccacatag gaatcgggaa accttgggat ctggatagaa
      961 tgcatggagg tgtgactatt ctttccccat acataaagga tgaaaaaggt gaatggtata
     1021 aagggacggc taatgccata ttccagaata ttgactacat agataaactt ggcccggagt
     1081 atatattgat tttatccgga gatcatgttt ataaaatgga ttattcaaaa atgcttgatt
     1141 ttcacaagca aaataatgcc gaagcaacta tttcagttat taatgttcct attgaggaag
     1201 cagacagata tggtataatg agtatagatg ataggggcaa aatatacaaa tttgaggaaa
     1261 agcctaaaaa tccccaaagt actcttgctt caatgggagt gtatatatat acctgggagg
     1321 tcttaaaaaa ttacctgatt aaggacaata ttgatgaaca atctgaacat gattttggga
     1381 aaaatataat tcctaacatg ctgaaggatg gaaggagcct atgggcatac gaatttagcg
     1441 gatactggaa ggatgttggg acaattcagg catactggga gtctaatatg gaacttgtca
     1501 aaagactgcc agaatttaac ttgtatgaac agacttggaa gatatattcg tttaatccaa
     1561 taatgcctcc ccattatgtt gattcaaaag gcagtgtaaa tacctctgtg gttgctgaag
     1621 gctgtatgat atatggaaca gttaagaact ctgtaatatt tccgggagtg gttatagaag
     1681 agggagcttt gatagaggat tcaattgtaa tgtctaaaag cagggttggc aagggggcat
     1741 atattaatca cagcattatt ggtgagggcg tttgtgttgg aagcaatgtg agaataggag
     1801 tgggtgaacc tgtacccaac gaatataaac ccggtgttta taactcaggt ataactgttg
     1861 tgggtgataa tgcctgcatt cctggtaatg tagtgatggg taaaaatgta atggtagata
     1921 ttggtgtggg gccagaagat ttttgttcgc tggatgtacc atcggggaag agtgtgttta
     1981 aaggcggtga ttgtgaatga aaaatacttt gggtgtcgta ttaaccggag gcaaatatca
     2041 gggcttgaag gacttatctg caataaggtc cggttctgct gtgcctgtgg gtggaaaata
     2101 ccgtgcaatt gattttgtat tatcaaatat ggttaattcg gggataaaaa aagttggtgt
     2161 tataacgcaa tacagtttca ggtctcttat ggatcactta ggttcaggta aggagtggga
     2221 tcttgacaga agatatgatg gactctttat attccctccc tctctatctg atgatgattc
     2281 ggggatatat atgggaagcg ctgatgcaat gtataaaaac ctgagttttt ttaagcggag
     2341 taatgaggaa tacgctcttc ttgcccaggg aaactgtgta tataaaatgc tatataaaca
     2401 aatgcttgaa taccatatag agaaaaatgc agatataacc atagcttata gagaaatgga
     2461 cgattataca cctgctgaat taacaagact gggcataata tctcttgata aagatgaaag
     2521 aattatagat tttcaggaaa agccattaaa tcctgcaact ttaaatggct ccataggtat
     2581 ttacatttta aaaagagagt tgctgatttc ccttttacag gagtgtgcag ctcatggcaa
     2641 ttatgatttt gttaaggata ttcttattaa aaaacttcct gaacttaaaa tctatgggta
     2701 taagttcagc ggttattgga gaaatttaag cagtgtaaga atgtattata gatgcaatat
     2761 ggaactcctt gatcctggtg tcaggcagga gttattcata aataatggaa agatctatac
     2821 taaagttaaa gatgaaacac ctgccaagta caatgctgaa gcagaggtta aaaattcaat
     2881 tgtagcagac ggatgcataa tcgaagggac agtggagaac agcattcttt tcagaggtgt
     2941 tatagtaggg aaaggttcag ttataaggga cagtataata atgcagagta catatattgg
     3001 agaaaatgta aatattcagc atgctatact ggataaaaat gtagttataa ataacgaaaa
     3061 gcagttcagg ggagattcag actggccggt tataatcggt aaggatgcaa tggtgtaagg
     3121 ttaataatgc aagaacagtt gattaagggc attaatggag gaatggaata tacaaatgaa
     3181 aagattctta aaaatattga cagctatagt tttagtggtt tctatagcct tatctatatc
     3241 attatccggt tgcggacggg gcaaaaaaga taaaattgag aaaagtatag atgataataa
     3301 tgtggaaaaa gcacaacctg agccagaaat agacctggaa aaaatcaggc ctgatgagtc
     3361 aggtaaaata atggttgtta tgttccataa ctttgttgaa tcatttactc ctacaaaata
     3421 tgataaaggt gaatatacaa ttacctttga tgggtttgaa aaacttctcc atactttata
     3481 taacaatggg taccgcctga taagttttaa agactacctt gaaaataaca taactgtcca
     3541 ggcagggtgt gtgcctatgg ttttcacttt tgatgatgga acatcaggcc agtttaactt
     3601 tatagaagaa aacggagagc ttaaggttaa taggaattca gcagtgggaa taatggaaga
     3661 tttcaataag ctgcatcctg attttggttt gaaaggaatg ttttttgtaa accttggttt
     3721 atcaactttt aatggtaaag gtgagctgca gcagcgatta aaatatttaa ttggtaaagg
     3781 ttttgaaata ggcaatcata ctttcaacca tataaatctt aaagatgcca aaagttctga
     3841 gaagatacag caggaactag gtaagaatca gaagaaaatg tatgaattgg tgcctggtta
     3901 caaaatgtat gctttttcat tgccatatgg agccccatca aatgatctta tgaaatacgt
     3961 cataaaaggt gagtttgaag gagaaaaata tgagaatctg gctattgttg aagtaggttg
     4021 ggatcctgca ctctctcctg taaacaaaaa atatgatcct gtttcaacgc atagagtaag
     4081 ggcacctggg attaaccctg aaaaatttga tttgaactgg tggttggaga acatctcaag
     4141 aaaagatgag tatgttagtg atggaaatcc aaagaaagtt acaattccta aagaaaaatc
     4201 tgatgttttg gatatggaga agcttaagga tacagaactt atcacatatt agaaaaaaag
     4261 atacatataa tttatatgaa gaaaatattg gcaaggggca taagttaagg ctctaagcac
     4321 cgtaacgata gcctctggaa aatatttccg catacataca cccctgaaag tctactctct
     4381 ttaattcgtt tgcaatttag gcggtgttgc tatataatag taatgtagga aagcgtatca
     4441 cgtttcggta aggagtgtta aatatggcaa atggaaaaac gaaaaaagtt caaaccgatt
     4501 ttgatgttaa gagaaaagct gttaagcttg ttattaccca tttaaagaaa aaagtatcaa
     4561 aagagtatat gggccgggaa catatcaata actggattgc cgaaatggaa gaacttatga
     4621 gcaagcctga ttttgatatg gtggaataca ttgatatgag aaaaaagctt aatgatgtaa
     4681 tcgaacgtac tcttgatgaa gaaatgaggt ataaaataag agattcatgg tatagcttgg
     4741 gtagagcgct ggataaaaag gttaagcaca gataattcaa catgacatga ttgcaacaaa
     4801 tacaaatgac gtgaaaaaac ccggcgaaca accgggtttt ttcatgttaa aactctaata
     4861 tattgataca tctcatcatt tcttatacta attttttcat actcccgaag gaaatattat
     4921 gatatggaag ggaaataatg taatattaaa atatacatcg aaacttcagc atttttgaga
     4981 aggggaagat atagttatgt tatttaaccg taatatcaga aatattttta atgaagttgt
     5041 taatgaaaaa aagcgcagca agctgacagt ggagaaaatg agggaagaac tgattagaaa
     5101 cctcaataaa aaaggtataa ggacttcaat tgttcaaatt aatatggatt ctgataattt
     5161 aatgataagg ctgcatattg atgataataa tgctgcattt agatcaagga tacagtgggg
     5221 atcctgagaa tccccactgt atgatgaata attaattaaa ttatactttc aaaaagctat
     5281 aacccttttt tattgacatt aatagacttt cttttgatgg ccctccaggt acatttctgc
     5341 ccgaaacaca tttctcgagg ctgatttcct tatagatatc atcctggatc aatgaagaaa
     5401 aagtttttaa ttgatcaata ctaagagcat caatagtttt actattatca atacagtaaa
     5461 gtactattct gcctgttatt tcatgagcat ccctgaaggg aacgcctttc tttacaaggt
     5521 agtcggcaac atccgttgcg tttgtaaatc ctccctgagc ggccttatac atgtaatcag
     5581 tatttacctt cattgttgct atcatattag taaaaacagg aaggcacaac ttaaccgtgt
     5641 caatagcgtc aaaaaccgct tctttatcct cctgcatatc tttgttgtat gcaagaggta
     5701 aagacttcat tgtagttagc atagccacaa ggtctccata tactctgccg gttttacccc
     5761 tcaccagttc tgctacatcg ggatttttct tctgaggcat tatactgctg cctgtgctga
     5821 aagaatcatc catttcaata aagcgaaact catgagaaga ccagagtatt aactcttcgg
     5881 aaaatctgct gagatgcgtc ataacaatgg ataggcagga tagcaattct atgatgaagt
     5941 ctctgtcact gacaccatca aggctgtttt ctgtaatagc attaaaaccc agttcctcag
     6001 ctaccatatg ccgatccagc ggatatgtag ttcctgccag agcgcctgac cctaaaggca
     6061 ttatatcggt tctttcatag cagtttccta gtctctcaat atcccttctg aacatttgga
     6121 agtatgccat catatggtgt gcaaaagtta ttggctgagc tcgttgaaga tgggtataac
     6181 ctggaagtat tgtatcgaga tgcttttcag ccatactcaa cagagtattt tccaggctga
     6241 tcagcatacc cttaatgctg tttatttcat ccttaagata catccttata tcaagtgcta
     6301 cctggtcatt tctgcttctg cccgtatgca gtttttttcc ggtatcacct attcttgata
     6361 ttaaaagctt ttccacattg gtgtgaatat cttctgcacc tatttcaaat tcaatcagcc
     6421 ctttttctat atcagcaagt atgtcaagaa gagtcttctg aataagctca gcttcttcca
     6481 aagggataat gccgcagctg cccagcattt ttacgtgggc aatgctgcca attatatcct
     6541 gcctgtacat tctgctgtcg aacctaatgg aggagttgaa atcatttact gatttatcag
     6601 tggctttaac aaaccttcca ccccaaagct tcatatataa tcatcacatc ctttttggtc
     6661 tagtgccagg agtctgctcg gaaagtccgg gctatcaaag cagaccgctg gaggaacgat
     6721 catggacttc attatgatac aaaaggattc aaaaaatgac gttcctcctg cacgagctcg
     6781 cttttttgag caaactcccg gataccactt gtaaaaaatt atttgttttt ctgattcatt
     6841 aaagcattta cttttagcgg caaaccaaac aggttaatga atccctctgc atctttctgg
     6901 ttgtatacat tatctctgcc aaaagtggat aactcctcac tgtataatga atatggggat
     6961 tttgaccctg caggcataca gttgccctta taaagtttca tcctgacagt acctgtaacc
     7021 gtttgctgag tgctgtcaac aaaagctgaa agagcttctc ttaatggagt gaaccactgt
     7081 ccaaagtaaa caagttcaga aaatttctga gctataaggt ctttataatg aagagtatct
     7141 ctatcaagac ataagagttc cagttccctg tgggcagcat ataaaattgt cccgccaggt
     7201 gtttcatata cacctctgga tttcattcct acaagcctgt tttccaccat atccataatg
     7261 ccgattccgt tttcaccgcc aattttattt aaaacttcaa taagttcaat cggtgaatat
     7321 tcaacaccat ttactttcac gggtatgcct ttatcaaaat agatctccac atatgacgca
     7381 ctatcgggag ctttttcagg tggtacaatc atctttagaa ttttatcgta ctgaggttca
     7441 ttccatggat cctccagatc ggaaccttca tgacttaagt gccaaagatt tctatccata
     7501 ctgtaattgt cctttttagt aacgggtatt ggtatgcctc tttcctgagc atagtcaata
     7561 gcatcttctc tggacttaat atcccaaatt ctccatggag ctattatttt tagatgtgga
     7621 gcaagagctt taacagtcaa ctcaaaccta acctgatcat ttcctttacc tgtagcacca
     7681 tgggcaatag ctgttgcgcc ttctttttca gctatttcaa ccattctttt tgcgataatt
     7741 ggccttgcaa aagatgtacc taaaagatat ttcccttcat aaatagcttc tgcttttaat
     7801 gtggggaaaa tatagtcagt aacaaactct tcttgaatat tttctatata gattttgctt
     7861 gctccagttt ttattgcctt ttctcttaaa ggctcaagtt cctctccctg acctacatca
     7921 gcagccatag caattacttc ataatcataa ttttccttta accacggtat tattattgat
     7981 gtgtccagtc ctcctgaata tgccaggata attttttctt tcttactcat ataaaggcct
     8041 ccttaaaaat atcttcaaat ttaaatgcta taataatatg ttactttact actaaaataa
     8101 tatggttaca tttgcaacca gcttatgtta caatagtaat gcatataatt tcacagagtt
     8161 gtgaattact tttgcattaa tataaatata taaatatatt tatattataa atactatgaa
     8221 tcattttgcc aaatagtaaa ttgttttata gcgcttgtta tttggcaatt gtttattatt
     8281 atacaacacg atgcataaat atgcaatact attaatataa aaatgggagt acatttaaaa
     8341 tatgaagaat aaattaaatg caccctaaaa ataacgaatt ttatttagag gacgtgaaac
     8401 aaactttgat aattatgggt attgatcccg gatttgcaat aacgggatat ggtataataa
     8461 aatatgaagg aaaccggttt acagttttag aatatgatgc gattataacg aagccatctg
     8521 aaaaatttgc agaaaggctc cttactctta acaataggat aaagaaatta ataaatatat
     8581 acagacctga agccttttca atagaagaac tgttctttaa caagaatgta aaaactgcta
     8641 taacagctgc tcaggggaga ggcgcagcaa tggttgctgc agcagaatgt ggtattagca
     8701 tctacgaata tacaccactt caagtgaaac aggctgtagt aggttatgga agagccgaaa
     8761 aaagtcagat tcaacaaatg gtaaaagtta ttttgaatct gccggaaatt cccaaacctg
     8821 atgatgtagc ggatgcttta gccatagcta tatgccatgc tcattcatac aaaatggaga
     8881 atattgccaa atgtcaggag gcaaaggata aatgataagt tatataaaag ggacactgga
     8941 atataaacat actgattatg ttattgttga agtacatgga ataggataca aggtttttac
     9001 ttccctgtca acaattcaat caataggaca agttggaagc caagtgaaat tacatacata
     9061 tttgcaggtg cgggaggaca atataagcct ttatggcttt gccactcctg aagaattgaa
     9121 aatgcttgaa cttttactta aggtgtccgg aataggacca aaagtttcta tttcaataat
     9181 gtcgaatctt tcaccttcga aatttagtct ggctgttatt actgatgatg taaaaacact
     9241 gacgaaagtt cccggggttg ggaacaagac agcacagagg ataattttag agttgaaaga
     9301 taaaataagg aaggaacagg gagatatttc ggattttgca agtacagagg attcgtctgt
     9361 ggttgacagg gcaggtatga aggcttctga agctataact gccttaatgg ttttagggta
     9421 tactgcgctg gaagcaaaca gagctgtttc tgccgtttac tctgaagagt tggaccttga
     9481 aaccgtaatc aagaattctt taaagaaatt aatatagctt attttacctg tgcttttaac
     9541 gcatacactg ttaaacggaa ttactgcatt aagaggagat gataaaatat ggaagagagg
     9601 cttgtcactt gcgaagcggt aggtgatgat caggatgaag caagccttag gcctagaagg
     9661 ctaaacgaat atattggtca aagcagagta aaggagaatc tctcaatatt tatagaagca
     9721 acaaagcaaa gaaaggaagc tttggaccat gtacttttgt atggccctcc aggattagga
     9781 aaaactaccc tggcaggcat aattgcttct gaattgggag tcaacttaag aattacctct
     9841 ggtcctgcaa ttgagaaacc tggagacctg gctgccatac ttactaattt gggagattat
     9901 gatgtacttt ttatagatga aattcacagg ttaagtagaa gcgttgagga aatcctgtat
     9961 cctgcaatgg aagactatgc gctggatata ataataggca agggacccag tgccagatca
    10021 ataaggctgg atctgcctaa atttacactt ataggggcaa ctacacgaat tgggctgcta
    10081 acatcgcctc ttagagatag attcggtgta ataaacagac tggagatgta ttcggccgaa
    10141 gaactaaaac tcattgtaaa gaggtcggca gctatactta atataggaat tgatgaagaa
    10201 ggtgctcttg aaattgcagt acgttcaagg ggaacaccca ggattacaaa cagaattcta
    10261 aaacgggtaa gagattttgc acagataaaa gctgatgggt tcataaatag agatgttgca
    10321 agaatggctc ttgatgccct tgaagtagat aacctggggt tggatggaat agatagggaa
    10381 atgctttttt gcataataga aaagttcgac ggaggtcctg tgggccttga tacgctggct
    10441 gcagctatca atgaagaggc cggtacaatt gaggatgttt at
//
LOCUS       DAOSDD010000157        10446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2424_length_10446_cov_5.397844, whole genome shotgun sequence.
ACCESSION   DAOSDD010000157 DAOSDD010000000
VERSION     DAOSDD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_10446_cov_5.397844"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..2344)
                     /locus_tag="PK733_13155"
     CDS             complement(251..2344)
                     /locus_tag="PK733_13155"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ11523.1"
                     /translation="MKPQVKYFKSILLLTVVIALVLSIVIFKKTDIYAGVRNKAEELA
                     DVIVNKCGVSGLQYTLISEGKIVVSDTAGVFDKNNTRVLNKNSLFGIGSTSKMFTTTA
                     ILMLSDEGAVDLDEPILTYIPEFKMADERYKQITVRMLLNHSSGILGSTFLDGFLFDY
                     PSALSHDNLLSNLETQKLKATPGEFSVYCNDGFTLAEIIVERVSGMRFTDYIKLNITE
                     PLKMSNTFTPQSDFDRNRLARTFVYNEETPVDTINIIGTGGLYSTAEDLCKFGQIFMS
                     NPTNKIAQNMLSENAKTATKQKEYKKGFGPDQKDGVFGYGLGWDSVDAYPYSGYNLQS
                     LIKGGDTQLYHSSLIVIPEHNITFAVVLSGGSSFFGQILGQELILETLLAEGNINKII
                     PPDLVTVPELSAMPAEKTAYSGVYASCESVKNVVITDEGKMTISSLSNTQIPDEIYLY
                     TAEGWFVSEDGSKKLTFVVETNGKTYIWVKWLLNIPELGQTSMSTYDYERVQPNSIDA
                     ETQNAWDSRNGTRYYIVNENPHAQQYYMPASSYFEIVTNEQMPGYATYFRIVDKNNGT
                     QDVQIPCLAGRDLATIEIAEIGGAEYLIMKDMVYISEKAMTDLPSGGNTFITIEDDGY
                     ARWFTVNPDDAGKTITVDIPENGSFAVYDTDACVYFSVVKGNRSVILPENGKVVFIGE
                     TPGVRFTASVKAEGN"
     gene            complement(2715..3113)
                     /locus_tag="PK733_13160"
     CDS             complement(2715..3113)
                     /locus_tag="PK733_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11524.1"
                     /translation="MKSKYSEIVKHIPSLEDHGHLYMYYGIPYSEECYIYDDEKEGNN
                     LIVSYECDDLCMAIADKFQYDYKWLDILHNNQIRLEKVFDIDVETQDFGIIVSLLLYL
                     VGSVTYEDKFIDALNNGYLIRLVKRLEYLS"
     gene            complement(3295..3969)
                     /locus_tag="PK733_13165"
     CDS             complement(3295..3969)
                     /locus_tag="PK733_13165"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HOJ11525.1"
                     /translation="MHFPAIYMLAYFRETRAFDYIIRIASLPDVQAYDLLGDTITEDF
                     GRIFASVCDGNIEPIKRIIEDSSLDEYVRDQALDSLLILLNNDVISREQLVFYFKELF
                     NGKLEVDYGSVWDSLPRICSLIHPKGLEDDIRKAVADGKVMEIIAGLDYMECQLHRPF
                     KEVLDELKEDKSYSLISERDVYSLERWVGVDLDEDFEDAYVEDFDDDEIPLNIPFKRE
                     NFVGGK"
     gene            4440..5129
                     /locus_tag="PK733_13170"
     CDS             4440..5129
                     /locus_tag="PK733_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ11526.1"
                     /translation="MKFFERIKQARTDADLTQTDVSEYFSKLGYSVKPYAISSWETGK
                     HKPDLEQFAVLCNVYKADAMYLLTGKNSQNHESLFYGLNQKGRTHALNYLNLLKSDPV
                     FTEEERIREVRAFRLYDISVSAGTGMYLDSDNYEEMTADDLIPDGTDYAVRVSGDSME
                     PKFYDGQILFIKEQGTLNDGEIGIFTLNGEAYVKKLLGDTLVSLNPKYEPIKLRESDA
                     FHVFGKVIGAN"
     gene            5201..6889
                     /locus_tag="PK733_13175"
     CDS             5201..6889
                     /locus_tag="PK733_13175"
                     /inference="COORDINATES: protein motif:HMM:NF026952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HOJ11527.1"
                     /translation="MSEPKNYEPYKIPPRNSIQGNEFDFISSKTDPAIFFKDQLFTEI
                     EIDSGINTTYEPAPIPQSIEPVMDEKRKIFNSMREISRYNHSSYFLNSKFYHKQAQYE
                     NSKIFYKQAVFMKDFEDDYTEKVPFSAYFPYYQLMGYEQLRTYFTWRTKVRSGKIENT
                     SLSYAFIYIYELLNNVGVNDPVDGLDKLMSFWTAFKVFDSTIEKYLLKWVKDYHIYYE
                     LPKPFKEFLCENKLQNHYPDIVGYLPEMNFNFEQLCGISKYNIKQSTFYGDKTNELIT
                     DCFDFTINKLKNIFADVGMELNDLIFKSSKSKSIWKPFNGALFYPNLKQPDRQVILCE
                     NEVYICSQNNWVLSTVITLESGRQLIGYILKQMEAVLRKVTKYKYNLSANLNVVNGEI
                     LQKLGAAGISLEKAVTDAVLEFYREKNKIVVSVNKFVLNKIRLEALDTQEKLIVPEND
                     FQMASLSFLEVSPADKEKENTLLPPADELVLPDRWTSLKNALNKTEIDALSAVLQGDP
                     NIKQFADENGIMLEVLADSINEKAFDHIGDNILELDDNMMIYDEYREKIMMMVG"
     gene            6886..8208
                     /locus_tag="PK733_13180"
     CDS             6886..8208
                     /locus_tag="PK733_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ11528.1"
                     /translation="MNMENQVPLRIANILINALKGGVVPRIGLEYITVGRTQEIAAIL
                     HDIEMIADGSASFRFIVGKYGSGKSFLLQTIRNYATAKGFAVMDADLSPERRFAGTKG
                     QGLATYKELIQNLSTKSKPDGGALPLILEKWISGIKASVKTQSEAEGDEFDLLVEKQI
                     YTVAGSLEGMVNGFEFAKAIVTYWRAYRQDDTIKKSNVLKWFRGEYASRKEAKEDLDI
                     NFIVTDETWYDFLKIFAVFLVSAGYKGMLVIIDELVNIFKISNSITRQNNYEKILTMY
                     NDVLQGKAKHIGFLMGGTPQCIEDKYKGVFSYEALRSRLAEGHFSTGDLKDLSAPIIR
                     LQMLMQEEMYVLVEKLRDIHAQLYNYIPSFGHDELVYFLTVEYNRVGAETHITPREII
                     RDFIELANILHQNPQKSMSEILGSNNFQMAKGGITEEEIHADFQEFEV"
     gene            8214..10427
                     /locus_tag="PK733_13185"
     CDS             8214..10427
                     /locus_tag="PK733_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ11529.1"
                     /translation="MNVFNRLAPFIQDFIYQSKWEELRGIQVAACEVIFDSDDNLLLS
                     SGTASGKTEAAFLPVLTELYQKPSRSVGVMYISPLKALINDQFKRLELLLTDSNMTVC
                     KWHGDASTTKKNELIKHPEGIIQITPESLESLLTNKRGACLQMFSDLRFIIIDEVHQF
                     MRDVRGVQLLCLLERLQMLTGVIPRRIGLSATLGDVSLAQTWLNTGTGRACAAPITEE
                     GKKRIKLHVERFVNLHDQRDVANSDSSDNTISTGGDMGNREHYKYLFKMTLDKKTIIF
                     TNSREETELVIANLKEIALQNKAPDVYRVHHGNVSALIRETTEDEMKSAGEKIVTGAT
                     VTLELGIDIGSLDQAVQIGAPLSVSSFAQRLGRCGRRGQVPQLLFTFVEDIRINSADT
                     LGPINWDFIRTIAIIELYTKDHWLEPIYPHNHAYNLLYHQTMSHLKSSGELSPARLAQ
                     TILSLGCFKQISQNEYKHLLTHLINIEQLQRTERGGLIIGREGEKIVNNHHFLTVFLA
                     PEYLLVKDENRVIGTVDKVYPVGIRFALAGLTWETVDVNVKSKVIFVKRVPGISVVDW
                     NVDFEAELHTTLVRKIRSILQTDMQYPYLSERCRERLTEIQYIARNSGILEYLITPLS
                     DTKYAVFPWVGTRQLLTLHYALMKRNIKNKLPWITCVYLEVNYNGTADELRSIIGDIL
                     RSNLNLYDLPLPDKVQINGKYNEFIPLDLLRKQFIEDYLDFEGLREGIRLSSGNI"
BASE COUNT         3197 a         2090 c         2073 g         3086 t
ORIGIN      
        1 gtatgttcca cagactggca tgctgacaga agatagactg tcagaataac taacgtaaat
       61 aaaactgcct taattcgata tttagccata atatgaaagt tcctccttat atacacagat
      121 tcacacctgc gttgttccat tcaatttagc tctgctccat cggcttaatc aaaaaacatc
      181 taacctgacc actcaccaac gctgacatcc aactcgacca ctcgcgggtt atgttcggtg
      241 ctgaagacac ttaattcccc tccgctttta cgctggcggt aaaacgaaca cctggggtct
      301 cgccgataaa aacaactttt ccgttttcgg gaagtattac cgaccggtta cccttaacca
      361 ctgaaaaata aacgcaggca tcggtatcat atacggcaaa cgacccattt tcgggtatat
      421 caaccgttat ggttttaccg gcgtcgtcgg ggtttaccgt aaaccagcgg gcatatccgt
      481 catcttcgat agttatgaag gtatttcccc cggacggcaa atcggtcatg gctttttcgc
      541 tgatatatac catatctttc ataatcagat attccgctcc gccgatttcg gctatttcaa
      601 tagtcgccaa atccctgccc gccaagcagg gtatctgaac atcctgcgtc ccgttatttt
      661 tgtctacgat tctgaaataa gtggcataac ccggcatttg ttcatttgtg actatttcaa
      721 aatacgaact tgcgggcata taatactgct gagcgtgagg attttcattc acaatatagt
      781 accttgtgcc gttccgactg tcccaggcat tttgtgtttc ggcatcaata ctatttggct
      841 gcaccctctc ataatcatat gtgctcattg atgtctgtcc tagttcgggt atatttaaca
      901 gccattttac ccatatgtag gttttgccgt ttgtttctac gacaaatgtt agctttttgc
      961 tgccgtcttc gcttacaaac cacccttctg ccgtatacaa atatatttcg tctggtattt
     1021 gtgtatttga aagcgatgat atggtcattt taccttcatc ggtaataaca acgttcttta
     1081 ctgattcgca gcttgcataa acgcccgaat aggcagtttt ctcagcaggc attgcggata
     1141 attcgggaac tgtgaccaaa tccggcggga tgattttatt tatattgcct tccgccaaca
     1201 atgtctcgag aatcagctcc tgccccaata tttgtccgaa aaaactagac ccgcccgata
     1261 acacaacagc aaaggttata ttatgttcag gaataactat caaactgcta tggtataatt
     1321 gcgtatcgcc gccttttatt aatgattgaa ggttataacc gctgtatgga tacgcgtcaa
     1381 ctgaatccca acccaggcca taaccgaaaa ctccgtcctt ctggtcgggt ccaaagcctt
     1441 ttttatactc tttttgcttt gttgcagttt ttgcattctc cgacaacatg ttttgcgcaa
     1501 tcttgtttgt agggtttgac atgaaaattt gtccgaattt acacaaatcc tccgcggttg
     1561 aatataatcc gcctgtcccg attatgttta tggtatcgac gggcgtttct tcgttatata
     1621 caaaggttct tgcaaggcgg tttctgtcga aatcgctctg aggtgtaaat gtatttgaca
     1681 ttttaagcgg ttcggtaata ttcagcttta tgtaatcagt aaacctcatt ccgcttaccc
     1741 tttcgacaat aatctccgca agagtaaagc catcgttgca gtaaacggaa aattctcccg
     1801 gcgttgcttt tagtttttga gtttctagat tcgataacag attatcatgg gatagagcgg
     1861 atggataatc gaataaaaaa ccgtcaagaa acgtgctgcc gaggatgccg gatgaatggt
     1921 tcaaaagcat tctcactgtt atttgtttat aacgctcgtc ggccattttg aattccggaa
     1981 tataggttaa aatgggttcg tcgaggtcaa ccgctccttc gtccgaaagc atcaaaatag
     2041 ctgtggtggt aaacattttg cttgtagagc ctataccgaa cagtgagttt ttgttcaaaa
     2101 cacgggtatt gtttttatca aaaacccccg ccgtgtccga tacaacaatt ttcccttcgg
     2161 atatcaaggt gtactgaagt cccgatacgc cgcatttgtt tacgataacg tcagctaatt
     2221 cctccgcctt gtttcttaca ccagcataaa tatcggtctt cttgaatatt acaattgaaa
     2281 ggaccagcgc aattactact gttaacaaca gtattgactt gaaatatttg acttgtggtt
     2341 tcattttcat tctccttgtc tttctttttg cgacatacag tataaagtct tttttatatc
     2401 atcagttttt agcaacatcc aacctgacca cacaacgtgg ctcgtccaag ggaacatatc
     2461 aaatttaata cttctgaaaa agtgcaccca ccttcataaa tatgtaatgt cttcgtatgt
     2521 tgtatccgtt gtattttcag ccaggctgcg tactagatat taagatatta atttattgct
     2581 tttaaatcta cttcaacctc aacaacggcg tacattccac aagtggtcgt tgtccgcata
     2641 atcttcattg acatatttaa ggaattcatc tttttcgggt atatagcgag gtttgtccgc
     2701 ttgatatcat tcctttaact caggtactcc agccttttta caagtcgaat caagtaccca
     2761 ttattcaatg catctatgaa tttgtcttca taagttacac ttcctaccag gtataacaaa
     2821 agagaaacaa ttatcccaaa atcctgcgtt tctacatcaa tatcaaagac cttttcaagt
     2881 ctaatttgat tattatgtaa aatatcaagc catttgtaat catattggaa cttatcggca
     2941 atagccatgc ataaatcatc acactcataa gatacaataa gattattacc ttctttttca
     3001 tcgtcatata tatagcattc ttcggaatac ggaattccgt aatacatata caggtgaccg
     3061 tgatcctcaa gactcgggat gtgttttact atttcactat atttactttt catggtaacc
     3121 tacctttctt cgttataatc tttggaatta gtcgacaccg gacaaatgta gaatctataa
     3181 atgactcttt acaatcattt actacattat atattatttt aagactttct ccaaacaatt
     3241 cttttaacaa tccctatata tttctgtata atttttacta cctttgtgcc cacattattt
     3301 tcctccgaca aagttttccc gcttgaaagg tatgttcagc ggaatttcat catcatcaaa
     3361 gtcctcaaca tatgcatctt caaagtcctc atctaaatca actccaaccc atcgctccaa
     3421 cgagtatacg tctctttcag aaatcagaga gtacgatttg tcttctttca gttcatccaa
     3481 aacctctttg aaaggcctat gaagctgaca ctccatatag tccagtccag caattatttc
     3541 cattactttt ccatctgcaa cagccttcct tatgtcatcc tccagacctt tagggtgtat
     3601 caaagaacat attcgaggaa gcgagtccca taccgaacca taatccacct caagttttcc
     3661 attgaacagc tctttaaaat aaaaaacaag ttgctctctt gagatcacat cattattcaa
     3721 gaggatcagg agggagtcaa gagcttgatc tctcacatat tcatccagtg aagaatcctc
     3781 aataatccgt tttattggct caatatttcc gtcacagact gaagcaaata ttcttccgaa
     3841 atcttccgta atcgtgtctc ccagtaagtc ataagcttgt acatccggca gggatgcaat
     3901 tcttataata tagtcaaatg ctcttgtttc acgaaaataa gcaagcatat atatggctgg
     3961 aaaatggatg ttgagaacaa aaaagaagtt gaatcaaaaa gaatgaagta gagaatgaca
     4021 tggtttataa tatggagata gcaatacgtg gcaaaatgaa ggaaatggaa cttaaaggaa
     4081 aaggagggtc gtatcccggc ctaaatgcgg caacaaaatt aaaattattt catttattga
     4141 ggatgatgcc acaataaaga agatactaat gcatctggaa ctctggatgc cttttatgct
     4201 gcaaaaatct cttgattttt aaagatatta ccagttttta actgcactac gaaaagtaaa
     4261 ttcctatctc tatctcttct ctctatctct aaggtagctt atatcacaat ctctcagtgt
     4321 tattgagggg attacaactt gatattaaca caatataaac aagcaattat tgtgaattac
     4381 acaattttta ttgatattct atacaatctg tgttacaata tttaccggag gcggatttta
     4441 tgaagttttt tgaacgcata aaacaagcaa gaacggatgc tgacttaaca cagacagatg
     4501 tttcagaata cttttcaaag ttaggatatt cagttaagcc ttatgccata tccagttggg
     4561 aaactggaaa gcataaacct gaccttgagc agtttgctgt cctgtgtaat gtatataaag
     4621 cagatgcgat gtatcttctg accggaaaga acagtcagaa tcatgagtcg cttttttatg
     4681 gtctaaatca aaaaggccgt actcatgctt taaactattt gaatttgtta aaatccgatc
     4741 cggttttcac agaagaggaa agaatcagag aagtcagagc attccgtctg tatgatatat
     4801 cggtatctgc ggggacagga atgtatttag atagtgacaa ttatgaagaa atgaccgctg
     4861 atgatttaat tccggatggt acggattatg ccgtccgtgt cagcggagac agtatggaac
     4921 ccaagtttta tgacggtcag attttattta tcaaagaaca aggaacgctt aacgacggtg
     4981 aaattggcat atttacactt aacggcgaag cttatgtcaa aaagctgctc ggggacacat
     5041 tggtttcatt aaaccctaaa tatgagccaa ttaaattgcg ggagtctgat gcttttcatg
     5101 ttttcggaaa agtaatagga gcaaattaac agagaaatcg aaaaagtctt gatgtgtatc
     5161 ctctttaaat atatattgtt gttaatggag agatgattag atgagtgaac cgaaaaacta
     5221 tgagccatat aaaataccac ccagaaattc gattcaaggg aacgagttcg atttcatttc
     5281 atctaaaacc gacccggcta ttttttttaa agaccagctg tttaccgaga ttgaaattga
     5341 cagcggcata aataccacat atgaacctgc acccataccc caatcaattg aaccggtaat
     5401 ggatgaaaag cgaaaaattt ttaattcaat gagggaaatt tcacgataca accattcctc
     5461 gtatttttta aattcaaagt tctatcataa acaagcacaa tatgaaaatt caaaaatctt
     5521 ttataagcaa gcagtattta tgaaagattt tgaggatgat tacactgaga aagtcccttt
     5581 ttcggcctac ttcccttatt atcaattgat gggctacgaa caactcagaa catattttac
     5641 ttggaggaca aaagtaagaa gcggcaaaat tgagaacact tctctttcat atgcgtttat
     5701 ctatatttat gaattgctta ataatgtcgg agtaaacgat ccagttgatg gacttgataa
     5761 gctaatgtct ttctggactg cttttaaggt ttttgattca actattgaga aatatctgtt
     5821 aaaatgggta aaggactatc atatctatta tgagctgccc aaaccattta aagagttcct
     5881 ttgtgagaat aaattacaaa accactatcc tgatatagtt gggtatctgc cggaaatgaa
     5941 ttttaatttt gaacagttat gcggtatttc aaaatataat atcaagcaat ccacttttta
     6001 cggcgataaa acaaatgaat taattaccga ttgttttgat tttacaatca ataaattaaa
     6061 aaacatattt gcagacgtgg gtatggaatt aaatgatttg atttttaaat cttctaagag
     6121 taaatctatc tggaagcctt tcaatggggc actgttttat ccaaacttaa aacaaccgga
     6181 cagacaggta atactttgcg aaaatgaagt atatatttgc agtcagaata attgggtgct
     6241 cagcacggta attacgctag aaagtggcag gcagctgatc ggttatattt taaaacagat
     6301 ggaagccgtt ttaagaaaag tcacaaaata taaatataat ctttccgcta atttaaatgt
     6361 agtcaacggc gaaatcctcc aaaaactcgg tgcagcgggt atttcccttg aaaaagcagt
     6421 cactgatgcg gttttggaat tttacagaga gaagaataaa attgttgtat cggtgaataa
     6481 attcgtttta aacaaaataa ggctggaagc gctggacaca caggaaaagc tgattgtacc
     6541 agagaatgat tttcagatgg cttcgctttc ctttttggaa gtctcgcctg cagacaaaga
     6601 gaaggaaaac actttattgc cgcctgccga tgaattggtt ttacctgaca gatggacaag
     6661 ccttaaaaat gcattaaata aaactgaaat agatgcactt tcagcagtgt tacagggtga
     6721 cccgaatata aagcaatttg ccgatgaaaa cggcatcatg ctggaggtgc tggcagacag
     6781 catcaacgaa aaagcgtttg accatatagg tgacaatatc ttagaactgg atgacaatat
     6841 gatgatatac gacgaataca gagaaaaaat aatgatgatg gtgggatgaa tatggaaaat
     6901 caagtgcccc tgcgaatagc gaatattttg attaacgcac tgaaaggtgg tgttgtgccg
     6961 cgtataggcc tggagtatat taccgtgggg cgcactcagg aaattgcggc aattttacat
     7021 gacattgaaa tgatagcaga cggcagtgca tctttccgtt ttattgtggg taaatacggc
     7081 agcggtaaaa gctttttact gcaaaccatc cgcaattatg ccactgctaa aggctttgcg
     7141 gtgatggatg ctgacctttc ccccgaacgg aggtttgcag gaaccaaagg gcagggactc
     7201 gccacataca aagagttaat tcaaaacctt tccacaaaat caaagccgga tggtggtgcg
     7261 ttgccgctca ttttggagaa gtggatttcc ggtattaagg catcggtcaa aacacaatcc
     7321 gaagcagagg gcgatgaatt tgaccttttg gtggaaaagc agatctatac agtcgcaggt
     7381 tcccttgaag gcatggtcaa cggtttcgaa tttgccaaag ccatcgttac ttactggcgt
     7441 gcttaccgcc aggatgatac catcaagaaa tccaatgtgt taaagtggtt tcggggcgaa
     7501 tacgcttccc gcaaagaagc caaagaggac ttggacatca actttatcgt caccgacgaa
     7561 acatggtacg actttttaaa aatctttgcg gtttttctgg taagcgccgg atataaagga
     7621 atgctggtca tcattgacga actggtaaat atatttaaaa tttccaattc aatcactcga
     7681 cagaacaatt atgaaaagat tcttactatg tataatgatg tattacaagg aaaagcaaaa
     7741 catatcggct ttttaatggg cggtacaccg cagtgcatag aggataaata taaaggtgtt
     7801 tttagttatg aagctctgcg ttccagactc gcggaaggtc atttctctac cggcgatttg
     7861 aaggatttat ccgctccgat catacgattg caaatgctca tgcaggaaga gatgtacgtt
     7921 ctcgtagaaa aattgcggga tatacatgcg cagctttata attatatacc atcattcggt
     7981 cacgatgaac tggtgtattt ccttacggtg gaatataacc gggtcggtgc cgaaacgcat
     8041 attaccccac gggagattat ccgcgacttt attgaattgg cgaacatcct gcaccaaaat
     8101 ccacagaaaa gcatgtcgga aattttaggc agcaacaatt tccagatggc gaaaggcgga
     8161 ataacagaag aagaaataca tgccgatttc caagaatttg aggtttaaat tttatgaatg
     8221 tttttaatag actcgctcct tttatacagg attttattta tcagagcaag tgggaagaac
     8281 tgcgggggat acaagtggct gcctgtgaag tgattttcga cagtgacgac aaccttttgc
     8341 tttcctccgg cacagcatcg ggcaagactg aagcagcttt cttaccagtg cttacagaac
     8401 tgtatcaaaa gccttccaga tcggtgggcg tgatgtatat ttcgccgttg aaagcactta
     8461 tcaacgacca gttcaaacgg ttggaactgt tacttacgga ttccaatatg accgtatgca
     8521 aatggcacgg ggatgcatca acaaccaaaa agaacgaact cataaagcat ccggaaggga
     8581 ttatacaaat caccccggaa tctttagaga gtctcctcac caacaaacgc ggcgcctgtc
     8641 tgcaaatgtt ttccgacttg cgttttatta ttatcgacga ggtgcatcaa tttatgcgtg
     8701 atgtacgggg tgtgcagctg ctgtgcctgt tggaacggct gcaaatgctt accggcgtga
     8761 ttcccaggcg tataggactt tcagctactc ttggggatgt atcattagca caaacatggt
     8821 tgaacacagg caccggccgt gcttgtgccg ctcctataac cgaagaaggg aaaaagcgca
     8881 tcaaactgca tgttgaacgt tttgtcaatc tccatgatca gcgcgatgtg gctaacagcg
     8941 acagcagcga taacaccatc agcacaggtg gagatatggg caaccgggaa cattataaat
     9001 acctttttaa gatgacgctg gacaagaaaa ccatcatctt taccaatagc cgtgaagaaa
     9061 ccgaacttgt catagcaaat ctgaaagaaa ttgccttaca aaataaagcg cctgatgtgt
     9121 accgtgtaca tcacggtaat gtttccgcat taatccggga aaccacagaa gatgagatga
     9181 aatccgccgg cgaaaaaatc gtcaccggag ctacggtgac gctggagctt gggattgata
     9241 tcggatcgct ggatcaggct gttcaaatcg gggcaccctt gtcggtttcc agctttgccc
     9301 agcgattggg acgttgcggc cgacgtggac aggtaccaca gcttttgttt acgtttgtgg
     9361 aagatatcag gattaattct gcagatacat taggcccgat aaattgggat tttattcgaa
     9421 ccattgcaat cattgagctg tatacgaaag atcattggct ggaaccgatt tatccgcata
     9481 accatgcata taatttgtta tatcatcaaa ccatgagcca tctaaaaagc agcggagaac
     9541 tttccccagc gagactcgct cagactattc tatccttggg atgttttaag caaatatcgc
     9601 agaatgaata taaacattta ttgactcatt taataaatat tgagcagtta caacgtaccg
     9661 aacgcggagg ccttatcatc ggccgggaag gtgagaaaat tgtaaacaac catcatttct
     9721 taacagtttt tttagccccg gaatatttac ttgttaaaga tgaaaaccgc gtaatcggca
     9781 cggtagataa agtatatccg gtgggcattc gctttgcgct ggcggggttg acatgggaaa
     9841 cggtagatgt caatgtaaaa tcaaaggtga tctttgtaaa aagagtaccg ggaatttcgg
     9901 tagttgactg gaatgtggat tttgaggccg agttgcatac cactcttgtc cgtaaaatcc
     9961 gcagcattct tcaaaccgat atgcaatatc cttatttaag cgaacgttgc cgtgagaggc
    10021 ttaccgaaat ccaatatatt gcacgaaaca gcggtatttt agagtattta attacgccgc
    10081 tttccgatac aaaatacgcc gtattcccct gggtaggcac acggcagctt cttacccttc
    10141 attatgcgct gatgaaaaga aatatcaaaa acaaactgcc atggataacc tgtgtatatc
    10201 tggaagtaaa ttataacgga acagcggacg aacttagaag tattattggc gatatattac
    10261 ggtcaaattt aaatttatat gatctgccat taccggataa ggtgcagatt aacggtaaat
    10321 acaacgaatt tattccgctg gatttattac gtaaacaatt tattgaggat tacttggatt
    10381 tcgagggatt acgggaggga atcagactat catcaggtaa catataaacg tgttattgat
    10441 gatcta
//
LOCUS       DAOSDD010000158        10403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2442_length_10403_cov_4.356881, whole genome shotgun sequence.
ACCESSION   DAOSDD010000158 DAOSDD010000000
VERSION     DAOSDD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_10403_cov_4.356881"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK733_13190"
     CDS             <1..277
                     /locus_tag="PK733_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11530.1"
                     /translation="NLVVIPAMLRQSILTLKITPVGLHSSQEDPNSGRIYIEPVIGDS
                     VLNHGLKAPLFRGTKNFFVRTALVCMVQHAKAIAETLDNIDYQKTVV"
     gene            complement(781..1404)
                     /locus_tag="PK733_13195"
     CDS             complement(781..1404)
                     /locus_tag="PK733_13195"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOJ11531.1"
                     /translation="MGKPWQVCFELGWKSFVKGSIPIGAVVVNETGEIISQGRSLQYE
                     KEGENGYVFHHKLSHAELNALLQVSEFDHPNIRNYTLYTTTEPCPLCFGALVMANVRN
                     LKFAARDRYAGSTDLNNISRYISSKNIKIEGPFSELEKVQITIQTTYELIRNDGAARL
                     LNVMKVDCPNSVELGVKLFEEKTLQKLRDKKMAVSEVFNHIAKLQNI"
     gene            1739..2011
                     /locus_tag="PK733_13200"
     CDS             1739..2011
                     /locus_tag="PK733_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOJ11532.1"
                     /translation="MSNAFEKMKVAMELRGFAEKTQKKYLYSLKRFSDFFGKPPEELG
                     YDEVREFLYHEIKVRKLSSYYVNASYGELSIRGTVLQHTCSTKVLI"
     gene            2017..2139
                     /locus_tag="PK733_13205"
     CDS             2017..2139
                     /locus_tag="PK733_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11533.1"
                     /translation="MKDLLGHGSIDTTCKYLHLTDSQILNVKSPLDVFGGDDNA"
     gene            2207..2320
                     /locus_tag="PK733_13210"
                     /pseudo
     CDS             2207..2320
                     /locus_tag="PK733_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            2332..2535
                     /locus_tag="PK733_13215"
     CDS             2332..2535
                     /locus_tag="PK733_13215"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ11534.1"
                     /translation="MHVLPSGFQRIRYYGILSNRNKKVKLTLCKKLTGVTTVSAKVKL
                     NARELILKLKGIDISIRLLPPVL"
     gene            complement(2510..2719)
                     /locus_tag="PK733_13220"
     CDS             complement(2510..2719)
                     /locus_tag="PK733_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOJ11535.1"
                     /translation="MSFKVTVIVQKDDNWYVAKCLENSVASQGKSVEEAINNLREALE
                     LYYEDSTPDMISPKQTFVTTLEVTV"
     gene            complement(3459..3593)
                     /locus_tag="PK733_13225"
     CDS             complement(3459..3593)
                     /locus_tag="PK733_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11536.1"
                     /translation="MENIINCVSHVSHELSVLKPDGGKHKTSDVRKLASIVYKSIPYL
                     "
     gene            complement(3946..4809)
                     /locus_tag="PK733_13230"
     CDS             complement(3946..4809)
                     /locus_tag="PK733_13230"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ11537.1"
                     /translation="MNSISTMIREDFTHVNAISIKQHGKTLLKEFYNGHKSVETYYIG
                     CIFKSFISALVGIAIKEEKIQSLEEKLINYYQEINLKDIDKSFSLLTLQEVMTKTSGI
                     IWPGIGEELPQSMHEVWRLKFKNIPGENFEYKPDPQIIIYLLEDLYEKDIISIANENL
                     FKPLGISKWEWTRDDIEGMKISVDDLERFGQLYLNKGTYKGKCFFTEEFYKKSVYPYS
                     LGGFPERKPYGYYWWIDKYQGIEYFSACGFGGQKLCIIPSIEASIVIISEMDRPHLEN
                     NAIIRNIFPLL"
     gene            complement(5223..5969)
                     /locus_tag="PK733_13235"
     CDS             complement(5223..5969)
                     /locus_tag="PK733_13235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ11538.1"
                     /translation="MVYPKMILFDYGHTLLHEPDFDTLRGEEALLKYVKSNKNNYTAK
                     DINDFSQGLFTEISVVRDMGFELHEWQFQRFLYDYLDIELSVSLPEAERIFWDNTSYG
                     DIMPNADKMIDYINSCHIRSAIISNIGFSGAALTERINRLLPNNRFEFIIASSDYMFR
                     KPNHYLFQLALKKARLNASEVWFCGDNVEADIEGAANVGIFPIWYENLMVENQWNKRM
                     EVLPKCKHLHIHEWNQLIEILEKQLYGKNN"
     gene            complement(6472..6726)
                     /locus_tag="PK733_13240"
     CDS             complement(6472..6726)
                     /locus_tag="PK733_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOJ11539.1"
                     /translation="MYKTIEDKCASICFGIVNNHPFVDGNKRTGIYTMLVLLEYNGIL
                     LRYTHKELIDLGLTIAEGRYKQENILKLSSLTESVSQKSR"
     gene            complement(6989..7501)
                     /locus_tag="PK733_13245"
     CDS             complement(6989..7501)
                     /locus_tag="PK733_13245"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ11540.1"
                     /translation="MINEQLSINTGNVDEAIEIMREAAKWLIDIGKPMWKLEDITKVK
                     LLENNKVDEFYVLKVDNDIAGAMILKWHDSFFWPDIKPGESGFIHKLSIQRKYSGTGI
                     SKKMIEYAVEECRKRNVGFLRLDCAGDRKELCNFYEKIGFKQVRRKMMGPFDVAFYVL
                     NVIPEEVFKG"
     gene            complement(7609..8202)
                     /locus_tag="PK733_13250"
     CDS             complement(7609..8202)
                     /locus_tag="PK733_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ11541.1"
                     /translation="MDFVTERLKKQVEFLIEIDKIKHIFRKTKLFNKSRFENDAEHAW
                     HLAVMALVMNEYSNKKIDISRVIKMVLIHDIVEIDAGDVIVYDTQMRTCAKEMENVAA
                     ERIFGILPEDQKEEFIGLWREFEEKKTPESKFAVTIDRFEPILQNQLTEYYTWKVHDI
                     TLDMLYDKNKHIQDGSKEIWDVVESIFSEAKSKGAIK"
     gene            complement(8547..9641)
                     /locus_tag="PK733_13255"
     CDS             complement(8547..9641)
                     /locus_tag="PK733_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11542.1"
                     /translation="MKKIIPNVVQFPTDAQCNGLICAFSSLMVSVNKENENTPVYCGQ
                     KRSACINCGNCNNKSILQRHYEAIYHDYITWSGIGLLWNDTDCTGCYEKTYTQYEFSY
                     DLPDRLDFVFKAAGYDYVRLNKKESKERLFEQIKASINNKMPALIKLGNGEDWSVVTG
                     YDTENQTLYGIDAHKHWAFKVAIPPDGYTEDELFFSDKWYDNLNCAIIITGKIDMLSF
                     SDIIKRIILALECKEHSKLEIKVMSALNQKNNDKHKLGVWLNELAGYTVERRWHAAES
                     ATAKIYHQTDSERNKQGLCFITDQYLKFHDLCWKVWGILGSSPKTGFRVADNVAERLG
                     KQEAIDGLKDMFAKLFEIDRNVLLKLREMI"
     gene            complement(9983..10156)
                     /locus_tag="PK733_13260"
     CDS             complement(9983..10156)
                     /locus_tag="PK733_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11543.1"
                     /translation="MNNDLYTNIGYIVVWCVKYIIIPIGVSVSAKIIADKLLRPQPEK
                     QRKKRLKKNRFTK"
BASE COUNT         3042 a         2212 c         1600 g         3549 t
ORIGIN      
        1 aaaccttgtt gtaattccag ctatgttaag acagtctata ctcacattga agataacacc
       61 cgttggttta cacagttccc aagaggatcc caactctgga aggatctata tcgaaccggt
      121 tattggcgat tcagtactca atcacgggtt aaaggcaccc ctattccggg gtactaaaaa
      181 cttctttgtg aggactgcct tagtttgcat ggttcaacat gcaaaagcca tagctgaaac
      241 ccttgataat atagattatc agaaaactgt tgtttaatca ataggaaatt tattccttaa
      301 ttcctatact tatattattt gttgcattca tagcgaagct ttgccatttt ttactggaaa
      361 acttctccta tcaattgcac ctttttcagt tttcaaagat cagttattct tttgcatctt
      421 acttcttatt catcacaatt cccttggtat atatttccaa tttgctttaa tttactgaac
      481 acctaccatt acataaataa tcctatgttt atacttatat aattttgcgg ttagaacatg
      541 tacaaacaat actacaaagt ctttcttact aaatcgtacc atagatggtg cgccttgcac
      601 ctgattacgt gtaacgtggt gcgggattta cgatgtgcct gaaccgcttg tacttgcatc
      661 cggtgaagga gtgtgccatt cttgctccag agcatttccc tcccatggct ttatgggtaa
      721 acatcgttct atgaagcgtg ggatcccacg aagccagggt gtgacacaga tggcacaccc
      781 ttaaatattt tgtaattttg caatatggtt aaacacttca gacacagcca tttttttatc
      841 tctcaatttt tgcaacgtct tttcttcaaa cagcttaacc cctaattcta ctgaatttgg
      901 gcaatctact ttcataacat ttagcaatct ggcagcacca tcatttcgta ttaactcata
      961 tgtggtttga attgtaattt gtactttctc gagttcggaa aagggacctt caattttaat
     1021 gtttttactg gaaatatacc ggctaatatt gtttaaatcc gtactccctg cataccgatc
     1081 tcttgccgca aatttcaaat tacgtacatt tgccatgact aatgcaccaa aacataatgg
     1141 acaaggttct gtagtagtat acagtgtata gttccgaatg tttggatggt caaattcgct
     1201 aacctgtagg agggcattta attcagcatg ggataattta tggtgaaata cgtaaccatt
     1261 ttctccttcc ttttcatact gaagacttct cccctgcgat attatctctc cagtttcatt
     1321 aacaactact gcaccaatag gtatggagcc tttaacaaat gacttccacc ctaattcaaa
     1381 acaaacttgc catggtttac ccaaatctgc ccaaaaaata atctcgacct cctttaatta
     1441 ccattaatcc tattaaaccg cgcaggatgc gttgccttta ccccacattt catagaacgt
     1501 agcgcgggat ttgcgacgtg cctgaaccgt gacccatgag gcctgttgtt tagcagcgct
     1561 tcgcgcccag tgaaggggta tggtgctcat gttccaaagc tttccacggt gcacccttgt
     1621 gcggactgct ttgctgaact aaaccgctct gtggtggcca gttaattatg gtacttcgtt
     1681 ttagtcgtac tttcatttac cattattaca caatattatt taaaaaggaa gtgtaattat
     1741 gtctaatgca tttgaaaaaa tgaaagtcgc tatggaactt agaggttttg ctgagaagac
     1801 tcaaaagaag tatctttata gcttaaagag attctctgat ttctttggta aacctccaga
     1861 agaacttggc tatgatgaag ttagggaatt cctgtaccat gaaatcaaag taaggaaact
     1921 atcctcctat tacgttaatg cctcttatgg agagctctcc ataagaggca ctgttttgca
     1981 acacacctgc tcaacaaagg tgctaatata gtccaaataa aagaccttct aggtcatggc
     2041 agtatagata ctacctgtaa ataccttcat ctcactgatt cgcaaatact caatgtcaag
     2101 agccctcttg atgttttcgg aggtgatgat aatgcctgaa ctgcaggata tttttaaaca
     2161 gtttggcgat gagtatcgta atactcataa tattcatatt gatggtctta aagctatgga
     2221 ttctattgca tcttgtcgca ctgctgcctt aggtggtcat gtggatgtct gtgatgactg
     2281 cggctgctat agtatatcct ataactcctg cggcaacaga aaaggtttct catgcatgtg
     2341 ttaccttccg ggtttcagag gataaggtat tatggaatac tgagtaatcg taataaaaag
     2401 gttaagctta ctctttgcaa gaagctaacc ggtgtaacta cagtaagcgc caaagttaag
     2461 ttgaatgctc gtgagctcat tcttaagctt aaaggtatcg atatatccat tagactgtta
     2521 cctccagtgt tgtaacaaat gtctgttttg gactaatcat atcaggtgta gaatcttcat
     2581 aatatagttc caaagcctct cttaaattat ttatagcttc ttctacagat ttcccttgag
     2641 aagcaacact attctccaaa cattttgcaa cataccaatt atcatctttt tgaactataa
     2701 ctgttacttt gaaactcata aactcacact ccaacaattc ttaataagtc tattttacca
     2761 caaaacaaac atacatttac attataaaat aaaatttata taaccttcta tatagaacag
     2821 gctttaaaaa atttaaaacc tgcttccgtt accttgatgt aacttacgat ttgcaaattc
     2881 ttaactcatc gaaaagcatt gttgcatcaa accctttaca cacctctctt ctatattttc
     2941 taccatagtt atgctgtaaa cttcaatagc aaagagcaat accacttcgc cccaaattta
     3001 taaacccatt aattttgtca tctttaataa taatattaga cagacaatag tcaccgtggg
     3061 aaaaaccaag ctcctcgtcg ggtctgtttt cttttaacca ttataaaaag tatttcaggg
     3121 ttttcaaatt cgtttacccc atacgttttt ggtatgctca aggttattct cctttatatg
     3181 taatttttct agtttgcatt ttgaaaggaa tttgcgaaca aactgttttt ctgctccatt
     3241 aacaaaaact atcttatagc acattttcac aaatttacta tttacatcca agctaattat
     3301 gttattatat aaataaggac ggtgacgctt gttgctgtca ggtttaaaat tggttgttat
     3361 tttttaaaac ggtttctatt gaaccgtttc tttctttcgc ttttcaggct gtggttgaag
     3421 cagctttccg gaaattactc tgatagctac tcctactact ataggtatgg aatggactta
     3481 taaactatag atgcaagttt tctcacatca ctagttttat gtttcccacc atcaggcttt
     3541 aggacactga gttcatgact tacatgactt acacaattta taatattctc cattttgaat
     3601 gtctccttat tcacaagata gttacctgtt atttctagtt tattcccatc tggatcttca
     3661 tcagtataga aaatatgcat agtaataaat ctaattagtt ctgtcgccta acgtcccgca
     3721 ttcgcgaaat gcctgagctt acaggtcaga cggaaacttt agatagctcc tatcttggtt
     3781 tctggctgac cgactcgatt agcgaaggca tatggtgtgc tgcctggact tggtttcagg
     3841 aacaaaatat tcagggctca gaccttgtac acctttacat gagtgctctt gttatctata
     3901 gtaccacgac ccccttgctc agagaacgcc ataggcggca ttcccttata ataatggaaa
     3961 aatgtttcta ataattgcgt tgttctctaa atgtggtctg tccatttcgg aaattataac
     4021 tatcgaagcc tctattgaag gtataataca taatttttga ccaccaaaac cacatgcact
     4081 aaaatattca atcccctgat acttatcaat ccaccagtaa tagccatatg gtttcctttc
     4141 tggaaaacct cccaaagaat acgggtatac agatttttta tagaactctt cagtaaaaaa
     4201 acattttcct ttatacgtac ctttattcag atacagttgt ccaaatcttt caaggtcatc
     4261 tacagaaatc ttcatgcctt ctatatcatc tctagtccat tcccacttac taattccaag
     4321 gggcttaaat agattttcat tcgcaataga aataatgtct ttttcataca aatcttctag
     4381 aagataaata attatctgtg ggtctggttt atattcaaag ttttctcctg gaatattttt
     4441 aaatttcaat cgccaaactt catgcatact ttgcggaagt tcttctccaa tacctggcca
     4501 tataatccca cttgtttttg tcattacctc ttgcagagta agtaaagaaa aagacttatc
     4561 tatatctttt aggttaattt cttgataata attaataagc ttttcttcta atgattgtat
     4621 tttttcttcc tttattgcaa taccaaccaa tgcagatata aaacttttaa agatacatcc
     4681 tatataatat gtctcaacgc ttttatgccc attataaaat tcctttaata gtgttttccc
     4741 atgttgcttt atactaatag cattaacatg ggtaaagtct tctcgaatca tggtagagat
     4801 actattcatc ttttgctacc tcacatacta taaaatattt ccttatttgc acattcagac
     4861 aactgtaaaa tatcaatctc cataaaacat attttatata tttccaccag gaatggcgga
     4921 cttttcgtgg cattgtagat aacggtttgc atttacgatg ctcggctgct tacaggaagc
     4981 cacttatgcc cgggcaccct ttgggtgggc taatgcggcg accgactcgg ttagcgggtg
     5041 agtgtgggca aaaatgacct catgccttac tcactgagtc ttgcttcatg atatgccctc
     5101 catgcaaatg cgttgttatc ggatgtcggg tgacccgtta ttcagaaagc aacatgatgt
     5161 tgtcccctca aacccacaaa aactctgctt aattactata tgatttctac gtagcacaac
     5221 atttaattat ttttaccgta aagttgcttt tccaatatct caattaattg attccactca
     5281 tgtatatgta gatgcttaca cttgggtaat acttccatcc ttttgttcca ttggttttca
     5341 accatcaagt tttcatacca tattggaaat attcctacat ttgctgcacc ttcaatatcc
     5401 gcctctacat tatctccgca gaaccatact tcagatgcat taagccttgc tttcttcaat
     5461 gctagttgaa aaagatagtg atttggttta cggaacatat aatcgcttga tgctataata
     5521 aattcaaatc tattgttcgg taaaaggcga ttaattcgct cggtcaatgc tgcacctgaa
     5581 aatccaatat tacttattat cgcgctacgg atatggcatg agtttatata atcaatcatt
     5641 ttatcagcat ttggcataat atcaccataa gaggtattat cccaaaaaat acgttctgct
     5701 tcaggtaagg atactgatag ttcaatgtca aggtagtcat ataggaatct ctgaaattgc
     5761 cattcatgta gttcgaatcc catatctcta actacactga tttctgtaaa caaaccttgc
     5821 gaaaaatcgt ttatatcctt tgctgtataa ttattcttgt tactcttcac atattttaac
     5881 aatgcttctt ctccacgtaa agtgtcgaaa tccggctcgt gaagaagagt gtgtccataa
     5941 tcaaacaata tcattttagg ataaaccatt tttacaccac cttacttaat tattaacaaa
     6001 gattatgaaa tattctacag gattgaactc ctttatactg ccgcaggaag ctaggtgccc
     6061 tattattcag agtgcgacag aatgttgcac tctcaaatca ctacctatgt aatagatagt
     6121 tctggaaatt taaaatctcc taaatcacaa tttatagttc ttacaaacaa agggtctgtt
     6181 tgatattctt gcatatttaa aattttcaaa aggcatataa cttttataat caccatttgc
     6241 ataacaatgc atgcacgcag ttggacagcc gcttatttga atatcaaacc acattaatta
     6301 ttattttcca tatggttcct cccgtataag ttattatgtt taatttcgaa tattctacta
     6361 ttgcgtccga cggctggagg tccgaacgcc caggcaggct gcatttcagc taatggctct
     6421 aactcctgaa gtgcctgagc ttacaagtca gacggaaacc ttagatagct cttatcttga
     6481 tttctggcta accgactcgg ttagcgaaga caattttaat atattttctt gtttatacct
     6541 accttcagca attgttaatc caagatctat taattcctta tgtgtatatc tgagtaatat
     6601 cccattatac tcaagaagca caagcattgt ataaatgccc gttctcttgt ttccatctac
     6661 aaacggatga ttatttacaa taccaaaaca aatgcttgca catttatctt ctatagtctt
     6721 atacaattct ttcccatttt aaatgtttta aaacctgttc tatataggat ggcgagcttt
     6781 ttgtgtgcca tatgtcagag aacgttcagc actcaagaca catccgaagc ttagatagct
     6841 cctatcttac tgaggtcgtg tggtgcagct ttcttcatgc tcgctctcgc aaagtcaact
     6901 tcttgctctt tcccgcccgc acccttgtgc agacgatttg ttttgtgaag tactgcagcc
     6961 cccgtaactc agagctagcc acggacggct agcccttaaa tacttcctct ggaattacat
     7021 ttaacacata gaaagcaacg tcaaacggcc ccatcatctt tcttcttact tgtttgaaac
     7081 caatcttttc ataaaaatta cataactctt ttctgtcacc agcacaatcc aaccttaaaa
     7141 atcctacatt tctctttcta cattcttcaa cagcatattc tatcatcttt tttgagattc
     7201 ctgtccctga atattttctt tgaatgctta gtttatgtat aaatccagat tctcctggtt
     7261 ttatgtcagg ccaaaagaat gaatcgtgcc actttaagat catggctcct gcaatatcgt
     7321 tatctacttt tagtacgtaa aactcatcta ccttattatt ctctagtaac tttaccttag
     7381 taatgtcttc taacttccac ataggttttc ctatatcaat caaccatttt gctgcttccc
     7441 tcataatctc tattgcctca tcaacattac cagtattaat tgataattgc tcattaatca
     7501 ctcatcttcc ccctaatact gtctgcaaaa tgcattttct catgaatacc acaaagccag
     7561 agccgaccac ggatgggaga ccctttaaca ttcttctttt attgataatt actttatagc
     7621 acctttgctt tttgcttcgg aaaaaatgct ttcaacaaca tcccatattt ctttagaccc
     7681 atcttgaata tgcttatttt tatcatatag catatctaat gtgatatcat gaactttcca
     7741 agtataatac tctgtcagtt gattttgtaa tataggctca aacctatcaa ttgtcacagc
     7801 aaattttgat tctggagttt tcttttcttc aaattctctc cacaaaccaa taaattcctc
     7861 tttttgatcc tctggcaata tgccaaatat tctctcagca gcaacgttct ccatttcttt
     7921 tgcgcatgtc ctcatctgtg tatcatatac aattacatca cctgcatcga tttcaacaat
     7981 atcatgaatc aataccatct tgattactct tgaaatatca atttttttat ttgaatattc
     8041 attcataact aaagccatta cagctaagtg ccatgcatgt tctgcatcat tttcaaaacg
     8101 agatttattg aataattttg ttttccgaaa aatatgctta atcttatcaa tttcaattaa
     8161 gaattcaacc tgtttcttta atcgttccgt tacaaaatcc attttaatat gccccttcct
     8221 gtttcccctt caaattgtag ctgtagagac taacttatca gacccgtgcc tggatgacac
     8281 ggctatcgca tgagctttca tgtaacatct ggtttgcccg acgctcctga accgtacaag
     8341 cgtagccttt cccgcgagcg gtgcttgcaa ccaggtgaag gggtgtggtg ctccttactt
     8401 cagagcctta tctcaaagca cctttgcggg caaacattgt taagtggagt tggcccaccc
     8461 gcagagcaag agccacaaga cgtggcaacc ttaaaacttt ctcgcaaatg ttatgctcat
     8521 taatctcaat aaaaaataag ccttttttat atcatttctc ttagttttaa taaaacgttg
     8581 cggtctattt caaataattt agcaaacata tcttttagac catcaatagc ttcttgcttt
     8641 cccaaacgct ctgcaacatt atctgcaact ctaaagcctg ttttaggaga agatcctaag
     8701 atcccccata ctttccaaca caaatcgtga aatttaaggt attggtctgt tataaagcat
     8761 aatccctgtt tgtttctttc actatcggtt tgatgatata tttttgcagt ggcgctttcg
     8821 gcagcgtgcc aacgtcgttc aaccgtatat cccgccaact cattcaacca aacaccaagc
     8881 ttatgtttgt cattattttt ttgattaagt gcagacatta cttttatttc gagtttggaa
     8941 tgttctttac actcaagagc taaaattatt cgcttgatta tatctgaaaa agaaagcata
     9001 tctattttgc ctgttataat aattgcacaa tttaaattat cataccattt atcagagaaa
     9061 aacaactcat cttccgtata tccgtcaggc ggtatcgcta ccttaaaagc ccaatgttta
     9121 tgggcatcta taccatataa agtttgattt tctgtatcat atcccgtaac aacgctccaa
     9181 tcctcaccat tgccgagctt tatcaaagca ggcattttgt tattgattga agcttttatt
     9241 tgttcaaaca atctttcttt gctttctttt ttgttcaaac gcacataatc atatccggcg
     9301 gctttaaaaa caaaatcaag cctgtcgggt aaatcatagg aaaactcata ttgggtatat
     9361 gttttttcgt aacaccctgt gcaatcggtg tcattccata aaagcccgat accactccaa
     9421 gtaatgtagt catgatatat agcttcataa tgtctttgta aaattgattt attgttacaa
     9481 ttaccgcaat tgatacaggc agagcgtttt tgtccgcaat aaacaggtgt gttttcattt
     9541 tctttattaa cagaaaccat aagagaagaa aaagcacaaa tcagtccatt gcattgggca
     9601 tcagtgggaa actgcacaac attaggaatt attttcttca ttacattgat cctcctcaat
     9661 ttaagattat ataaataact catacaaccg tccccaagtc aatccgacgc atgacgcggc
     9721 ggcctttcgg ccaatttcac ttaacgacca ggcactcgtg aagtatctaa gcttacaagt
     9781 caggcggaaa ccttaaatag ctcttatctt ggtttctagc tgacagactc ggttagcgaa
     9841 gacatttggt gtactgcctg gacttggtat cagaaacatg tttttcagaa ctcagatcat
     9901 gtacaccttt atgcaaatgc ttttgttata tgatggactg ggacccgcgt agccagaagc
     9961 agacacggac gtctgctcct ttttattttg taaaacggtt tttcttaagc cgtttttttc
    10021 tttgcttttc aggctgtggc cgaagcagtt tatcagcaat aatttttgcc gaaacactaa
    10081 ctcctattgg tatgattatg tatttaacac accagacaac aatataaccg atgttagtgt
    10141 ataaatcatt attcatccaa ccacctcctt cctttgcata ttccccgcag caactataac
    10201 gtcgccatcc ttatatatgg aaatacaaag aaaggacctt taaagcaaat cacccgtctc
    10261 aaacctgatt gcttattacc tgtccattat actatataca atttataatt gcaatatatt
    10321 ccagaacatt tgtttatacc ttaaattcta tttgataatt attttatgat tttttataga
    10381 accctaaaaa cacctgcgaa acg
//
LOCUS       DAOSDD010000159        10287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2473_length_10287_cov_5.010751, whole genome shotgun sequence.
ACCESSION   DAOSDD010000159 DAOSDD010000000
VERSION     DAOSDD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2473_length_10287_cov_5.010751"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK733_13265"
     CDS             complement(<1..484)
                     /locus_tag="PK733_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11544.1"
                     /translation="MTNKNIIINMNKNLNENINENMNRVAGNASVRLIDKFIRAVIWF
                     FSFIALGFLIWIIVFVFIKGIPHISLSFLTSPNNGKNEGIWPMIINTLFIVVTSLAIS
                     IPVGIGAAIYLVEYSKPGKVINTIRFAVETLAGIPSILYGLFGLIFFVTIMKLSFSIL
                     A"
     gene            complement(489..1328)
                     /gene="pstC"
                     /locus_tag="PK733_13270"
     CDS             complement(489..1328)
                     /gene="pstC"
                     /locus_tag="PK733_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOJ11545.1"
                     /translation="MFFFCAFISVFSLALITAYIFIKGTPIIFKIGLSDFIFNKDWNP
                     DPVRGNPSFGIFSMIAASMLATFGSVIAGSFLGILTAVFLAEIAPLFAVKIFKPAIEL
                     LAGIPSVVYGFFGLVMIVPLIRKYLGPPGNSLLAVIIILTFMILPTIVNISEAAIKAV
                     PAEYKEGSLAVGATHIQTIFKVVLPAAKSGILASVILGMGRAIGETMAVILVAGNSTL
                     MPESLLSPVRTLTANIALEMGYAFGDHESALFATGVVLFIFIMTLNIIFNMLVNKAGG
                     HHD"
     gene            complement(1618..2478)
                     /locus_tag="PK733_13275"
     CDS             complement(1618..2478)
                     /locus_tag="PK733_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ11546.1"
                     /translation="MKFTGFNTKSNIRFTAMLCLAFVTLPLIFSSCNSANTSTDSGKL
                     SSKLEIIGSTSTQKLFETLADEFVIKNPKVKINYQGVGSSKGIKAVKDGTGDIGTSSR
                     ELKEEEKSWGLKEYVLAKDGIAIAINPGNQKNELTVDQITKIFKGEITNWKDIGGKDR
                     KIIVLSREAGSGTRGAFEELLKIEDQVVKEALVYDGNGPMKAAIASTEDAIGYISFGY
                     MDDSVKTLRIDGVEPTIENVLSGSYPLSRPFIIMTKGEAEGLAKSFIDFALDKEGQSI
                     VKDEGYIPIK"
     gene            2705..3202
                     /locus_tag="PK733_13280"
     CDS             2705..3202
                     /locus_tag="PK733_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HOJ11547.1"
                     /translation="MRVYEYSREKAVAYAHKWAYARNPAYYDFENIGGDCTNFASQVI
                     HAGSGVMNYTPAYGWYYINANNRTPSWTGVNYLYRFLTSNSGPGPFAEEVDIKDIKPG
                     DIIQLSFVGNGFFNHSPVVVHTGSPPELGNILIATHSDDQDYYPLIGYRWVDIRFIHI
                     KGVIG"
     gene            3405..5684
                     /locus_tag="PK733_13285"
     CDS             3405..5684
                     /locus_tag="PK733_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOJ11548.1"
                     /translation="MSNNQENYLEESEYLKQTLNSTQQELSKQYELLSGKEKSLIAAR
                     KDMWENSGHSSLDFDKLTEMNNYLQEVNSQARSYKDTAAKIKKYEKVLYSPYFGRFDF
                     IENGFGEREKIYIGLHSVIDSKNHEIYVYDWRAPISSIYYRYEPGKASYKAPAGNIEG
                     DVILKRQYKIENSELKYFFDCSVKIDDEVLQEVLYKNASDKMKNIVETIQRDQDIIIR
                     DTDNELLIVQGVVGSGKTSIAMHRIAFLLYHGLHSELTSENMLIISPNTIFSQYVSSV
                     LPELGEENVNQIAFSDYTVKTLKKKGRVETKDQYFETLISSQDTEKVLLKKQSSKFKG
                     SHLFVKILDRFIAYFEHRMIHFDDVYFARKIIESRQRLKNIFLDNKINRPAAERLKRI
                     ENMMLEAIRPLQKDRLRRIEKIIEVHGGHEFEEREFARLLSIKESRIFLNKIRKFTEV
                     DYYSVYKLLFKERWLFETLSQGLEMPDNLAQIIEFTWDNLEKDFISYEDSAPLLYLKF
                     RLEGSNEFSDIRQVVIDEAQDYTPMHYEVFNLLFKDANYTVLGDVNQSMDKEIDLSFY
                     DTIVKIFNKKKSVIVALNKSYRSSYEISTFNQRLLSKKQDIIPFERHEAEPLIVFQST
                     LDEMYKTIIKDAYSHLKQGYKSIAIICKTTCESENVYKKLREMRKLLKRQNKMYEDIN
                     LYTDIKLIDSNSDEIEQGILVIPAYMAKGLEFDVVLVFNVSQDNYHTELDRRLLYIAC
                     TRALHRLVLYYTGETMIKL"
     gene            complement(5941..6894)
                     /locus_tag="PK733_13290"
     CDS             complement(5941..6894)
                     /locus_tag="PK733_13290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ11549.1"
                     /translation="MGKSFITVIIPVYNEENQIFENINIIRDYLSKTEMNFEILLIDD
                     GSTDDTWFVLKMLTETHPGIKALRLSRNFGKEAALCAGLEAAVGDACIIMDSDLQHPP
                     ELIPEMVRLWKEEGYEVVEGVKALRGKESPVNKAGANLFYYILSKFSGFDMNQASDFK
                     LLDRRVVQAWQSMNEKNTFFRGMSAWVGFNRISIPFNIRKREKGTSKWSLLKLFKLAI
                     NAITSFSSFPLQIVTFMGLVFLAGSLILGIQTLYMKIKGIAYSGFTTVILLLLIIGSA
                     LMISLGIIGTYIAKIYEEVKQRPRYIVAEEIESRKELSVKQ"
     gene            complement(7041..9827)
                     /locus_tag="PK733_13295"
     CDS             complement(7041..9827)
                     /locus_tag="PK733_13295"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ11550.1"
                     /translation="MRYNNLIDNNLRCNRYNKHIIKRTGLLIRKNYVILLSFFLPAII
                     LELAYISCGIFPFGKWDLLIIDLYHQYAPFISDLQDRLRSFSSLLYSWSGGLGTNYLP
                     LYAYYVASPLNLITVLFPKEYLAEAVLVLTLLKVSLAGACFAYYLKGVHHEQSLVTVA
                     FSLLYALSGYVLVFSWNIMWMDAIYLLPLIMLGLVKLVRDSKGLFYCITLAVALLSNF
                     YIGFFICLFTMLYYPVLLFQYNSIKKPVLLLKKTAKFAGYSLLSAGLSAVLLLPTYFA
                     LKTTSAADDVLPKTITHYFDLFDYITRHFTAATPSIREGMPNLYSGIVVLILLPVYFL
                     CKNIRLKEKLWNLALLAVLLVSFNINILNFIWHGFHYPNQIPYRFSFVYIFLILSMCY
                     DAFKRLNEFTSKQIGAICMSVLGIIVISQKFDNLSLEYAIIYASIISIILYAVAFALD
                     RSYKIRHSHLPRALFFFLAITAELVSSTIITTIKIDSLEGYSFRDGYSSGKEVAQIRE
                     WIRDIEKKDKDFYRMEVLPPKTTNDPYLYNYRGLSIFSSTLPVKPVKMFENLGYHSNS
                     INSYEYEGSTAVLDSLFGIKYLIYRSKNIEEHLYETVAATEEMTVYKNPYTLTLGFQA
                     PDELKKFHSTWTNPFDAQNALINYISGIKDILVPIDQEQGAQDNLTFSSSGTRYYSYN
                     RTNKEVSSTAKVKFSVKEDQQVYLYFKAPYNMEGSGFVKIDDKKVDFNPKHSSIINLG
                     FCKSGTLPELQLTFEKSAPESGRFEIYVCGLNQQEFENAMSLIREKSMTIESFKDTRI
                     RGHIETSTDGLMVMTIPYNKGWHVKVNNQKVETQAIDDCLLAFELPAGSNKIELWFIP
                     EKFFIGSMITLASILILALGYLGRSIWKKREMHGDGSHILQGAEQRDDSPVSEHTENA
                     EDGEHMDGSHVSML"
     gene            complement(9887..>10287)
                     /locus_tag="PK733_13300"
     CDS             complement(9887..>10287)
                     /locus_tag="PK733_13300"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HOJ11551.1"
                     /translation="LRNIYTVKSNLEVGLGRSDIILIPKDKKYHPIIMEFKALGKEGI
                     EKLAAAKKPTVNGDNTDSGDNPSSWGNPDSGDNRESLALETIAYEAIKQIKDRKYYKV
                     FEGEGFKDVLLMGIGHHKKQCAVKTEYFKI"
BASE COUNT         3322 a         1826 c         1877 g         3262 t
ORIGIN      
        1 ctgctaatat tgaaaagcta agtttcatta ttgtaacaaa aaatatcaaa ccaaacaaac
       61 catacagtat tgaaggaatt cctgccaaag tttcaactgc aaaccggatt gtatttataa
      121 ctttaccggg ctttgaatat tctaccagat atattgcagc acctattcct acagggattg
      181 aaatagcaag tgatgtaacc actataaaaa gggtatttat tatcataggc caaatgccct
      241 catttttgcc gttattagga gaagttaaaa agctcaagga tatatgaggg attcctttaa
      301 taaaaacaaa tacgattatc catattagaa agcctaaggc tataaatgag aagaaccaga
      361 taacagctct tataaattta tcaattagtc tgacagacgc attaccagct actctattca
      421 tattttcgtt tatattttcg tttaaatttt tattcatatt tataataata tttttattag
      481 tcatgttatt aatcatggtg tccacctgcc ttatttacaa gcatgttgaa aataatgttt
      541 aaggtcatta tgaatataaa cagtactaca cctgtagcga aaagagcact ttcatggtca
      601 ccaaaagcgt aacccatttc taaagcgata ttggcagtaa gggtgcgtac cgggcttaag
      661 agagattcag gcatcagggt agaattccct gcaacaagaa tcacagccat tgtttctcca
      721 attgccctac ccattcccag aattactgat gccagaatac ctgatttggc agcaggcaga
      781 accactttga atatggtttg tatatgggtc gctccaactg caagtgaacc ttccttgtat
      841 tcagctggga ctgcttttat agctgcttct gatatattaa caattgttgg cagtatcata
      901 aaggtcagta tgatgattac agccagcagg ctgtttccag gagggcccag gtattttctt
      961 attaaaggga caatcatcac gagtccgaaa aatccatata ctacggatgg tataccagca
     1021 agaagctcaa ttgcaggttt aaatatcttt actgcaaaaa gcggagcaat ctctgcaaga
     1081 aataccgcag tgagtatccc gagaaaactg cctgcaataa ctgatccaaa tgtggcaagc
     1141 atcgatgctg caatcattga gaaaattcca aaggaaggat tccctcttac agggtcaggg
     1201 ttccagtctt tattgaatat aaagtctgat aggcctattt taaatattat tggagtacct
     1261 ttaataaaaa tgtatgcagt tatgagagca agggagaaaa cagagataaa tgcacagaaa
     1321 aagaacattt tctcagccag ccattctttc actttattcc acttaaactt ggtgttacca
     1381 attgctttgg tatttatagt tgttttactt ttaacatcct ttggcgatac tttgccaaaa
     1441 gcttcacttt ttacataagc cattgtattt atttcctcct tagccacatg ggtagacaca
     1501 gacacatggg gacggttctc ctgtttctta gatacatggg aacagtcctc ctgtttgggg
     1561 acagttctct ttaaaatagc ttgagacagt tctatcttga cttcacattt caaatttcta
     1621 tttaattgga atatatcctt catcctttac aatgctctgt ccctctttat caagtgcaaa
     1681 atctatgaaa gatttggcca acccttctgc ttcgcctttt gtcatgatta taaaaggcct
     1741 tgacagggga taggagccgg aaagtacatt ttctatagtt ggttcgacgc catcaatcct
     1801 taaagtcttt actgaatcat ccatatagcc gaaagatata tagccgatag catcttcagt
     1861 gcttgcaatt gctgctttca ttggcccgtt tccgtcataa acaagagctt cttttacaac
     1921 ctgatcttca attttcagta attcttcaaa ggctcctctg gtacctgatc ctgcttcacg
     1981 acttaaaact attattttcc tgtccttgcc tccaatatct ttccagttgg taatttcccc
     2041 cttgaaaatt tttgttattt ggtctactgt aagttcattt ttctgattcc cgggattaat
     2101 tgctatggct atgccgtctt ttgccaggac atattcctta agtccccagc ttttttcttc
     2161 ttccttaagt tcgcgggatg aagtaccaat atctcctgta ccatctttta cagccttgat
     2221 acctttactt gacccgacac cttgatagtt aatttttact tttgggttct ttatgacaaa
     2281 ctcatctgca agagtttcaa atagcttttg agtggaagtt gagcctatga tttcaagctt
     2341 actactgagc tttccactat cagtagatgt atttgctgaa ttgcagcttg aaaatataag
     2401 tggaagcgta acaaaagcca aacataacat tgcagtaaac cttatattgc ttttagtatt
     2461 gaaaccagta aatttcataa atattaattc ctccatttca tttttaattt aaataaattt
     2521 tgttatttgt tttaaaatga attcatttta tggttttact atacaaatac agggttaaag
     2581 ctgaattaac tatttgttaa atccatgtta aatgtagtga attgtgattc attaagcaca
     2641 ttaactatta caagtgaata atgtgtatat atatctaata tatatggggt gcgctacagg
     2701 aagtatgagg gtatatgaat atagcagaga gaaggcagtg gcatatgctc ataagtgggc
     2761 ttatgccagg aatcctgcat attatgattt cgaaaatatt ggcggtgatt gtaccaactt
     2821 tgcctctcag gtaattcacg ccggaagtgg ggtaatgaac tataccccag catatggatg
     2881 gtattatata aatgcaaata acaggactcc atcctggaca ggggtaaatt atctgtacag
     2941 gtttttgaca agcaatagcg ggcctggacc ttttgcagaa gaggtggata ttaaagatat
     3001 aaagcctgga gatattattc aactgtcatt tgttggcaat ggatttttta atcattctcc
     3061 tgtggtagtt catacaggta gtcctcctga acttggaaac atacttatag ctactcattc
     3121 agatgatcag gattattacc cattaatagg atacagatgg gtagatatcc gatttattca
     3181 tataaaaggt gtgatagggt aatgcaccta gtacggttaa aatattgtaa gataaaaaat
     3241 acttgatata attaatcaaa aaggttataa tgtaattctg agcataacta acccaagttt
     3301 attataatac tacaatacaa ttgtataata cattcaatgt tttgtcaagg ggttattttt
     3361 taaaaataaa ataatatgtg ctttaaagat tggagcgtgc gtatatgagt aataatcagg
     3421 aaaattacct ggaagaatca gaatatctca aacaaactct taattctact caacaagaac
     3481 tgagtaagca atatgagttg ctgtcgggta aagaaaaatc tcttattgca gccagaaaag
     3541 acatgtggga gaatagtggg cattcatctt tagattttga taagcttaca gaaatgaata
     3601 attatcttca ggaagtcaac agccaggcaa gaagctataa agatactgct gcaaagataa
     3661 aaaaatatga aaaagtgctt tattcccctt actttggcag atttgatttt attgaaaatg
     3721 gctttggaga aagagaaaaa atatatatag gattacatag tgttatagac tcaaagaatc
     3781 atgagatata tgtttacgat tggcgtgctc ctatttcaag catatactac cggtatgaac
     3841 cagggaaggc ttcttacaag gccccggcag ggaatattga aggtgatgta attcttaaaa
     3901 gacagtataa aatcgaaaat tcggaactaa agtacttttt tgattgcagt gtcaagatag
     3961 atgatgaagt actgcaggaa gtgctgtaca aaaatgcatc agataagatg aagaatatag
     4021 ttgaaaccat tcagagagac caggatatta ttataagaga tactgataat gaattgttaa
     4081 ttgtgcaggg tgtagtaggg agtggaaaga catccattgc catgcacaga attgcatttt
     4141 tgctttatca tggactgcat tcagagctta cctctgaaaa catgctgata atttcaccaa
     4201 acactatttt cagccaatat gtatcaagtg tcctgcctga acttggagaa gaaaatgtaa
     4261 accaaatagc attctctgat tatacagtaa aaactttaaa gaaaaaggga agagtagaaa
     4321 caaaggatca atactttgaa acattaatat cttcacaaga tactgaaaag gtgcttttga
     4381 aaaagcaaag cagtaaattt aaaggatctc atttatttgt aaaaattctc gacaggttca
     4441 tagcatattt tgaacatcgt atgatacatt ttgatgatgt ttattttgca agaaaaataa
     4501 ttgaatcacg gcagcggttg aaaaacatct ttttagataa caaaataaac cggccggcag
     4561 cagagagatt aaaaagaatt gaaaacatga tgctggaagc aattcgtcct ttgcagaagg
     4621 ataggctgcg gagaattgaa aagattattg aagttcatgg tggacatgaa tttgaagaaa
     4681 gggaatttgc tcgtctcctt tctataaaag aatcaagaat ttttttgaat aagattagaa
     4741 agttcacgga ggttgattat tatagtgttt ataaactgct ttttaaggaa agatggttat
     4801 ttgaaacact ttcgcaaggc ttggaaatgc cggataatct tgcgcaaata atcgagttta
     4861 cctgggataa tctagaaaaa gattttataa gttatgagga cagtgcaccg ttgttatatc
     4921 ttaaatttcg gctagaaggc agcaatgaat tttcagatat aagacaggtt gtaattgatg
     4981 aagctcaaga ttatacccct atgcattatg aagttttcaa cctgttattc aaagatgcta
     5041 attatacagt tttgggggat gtcaatcaat ctatggacaa agaaattgac ctctcatttt
     5101 atgacactat agtaaaaata tttaataaga agaagtcagt aatagtcgca ttaaataaaa
     5161 gttacagatc atcctatgaa ataagtactt ttaatcagcg attacttagc aagaaacagg
     5221 atattatacc ttttgagaga catgaagcgg agccgttgat agtatttcag agcactctgg
     5281 atgaaatgta taaaacaatt ataaaagatg cttattctca tttgaaacaa ggttataaat
     5341 ctatagcgat tatatgtaaa accacctgtg aatctgaaaa tgtttataaa aaattgaggg
     5401 aaatgagaaa actgttaaaa cggcaaaaca agatgtatga agatattaac ttgtatacag
     5461 atataaagct tattgactca aatagcgatg aaattgaaca gggtattttg gttattcctg
     5521 catatatggc aaaaggactg gaattcgatg ttgttcttgt ttttaatgtc tctcaggaca
     5581 attaccatac cgaattagat agaagacttt tatatattgc atgcacaaga gctcttcaca
     5641 ggctggtact gtattatact ggagaaacca tgattaaatt gtgaaaagta taggttatga
     5701 aaaacatgga gataagaagc ttctttccac ctataaagat aagaggcttc ttggcttttt
     5761 gattgggctt tgtactatta ttctcaaaaa gttaacaaaa aaacaaactt ttttgctcaa
     5821 aaccatgtag tataagcatt acatggtagt ttttgagaat atatagtata ttaatttatc
     5881 gcggcgtatg tttatttgtt tgcagctaac cgctgcgata cgcatattta acggtcaact
     5941 tcattgttta actgacagct ctttcctgct ttctatttcc tctgccacaa tatacctggg
     6001 tctttgtttg acttcctcat atattttggc gatgtaggtc ccgataattc ccaagcttat
     6061 catcagggca cttccaatta tcaaaagtag aagaataact gttgtgaaac cgctataagc
     6121 tatacctttg atcttcatgt ataaagtctg gattccgagt atcagcgaac ctgcaagaaa
     6181 gaccaacccc ataaaagtaa ctatttgaag gggaaatgat gaaaaggagg ttatggcatt
     6241 tattgcaagt ttgaaaagct taagcagtga ccacttggat gttccttttt ccctttttct
     6301 tatattaaaa ggaatactta tcctgttaaa cccgacccat gctgacattc ctctaaaaaa
     6361 ggtattcttt tcattcatgc tttgccatgc ctgaacgact cttctatcta gcagtttgaa
     6421 atctgaagcc tggttcatat caaatccgga aaacttgctt aatatatagt aaaacaaatt
     6481 agctccagct ttattaaccg ggctttcttt tccgcgtaaa gctttaactc cttctactac
     6541 ttcataaccc tcttctttcc aaagacggac catttcagga atcaattcgg gcggatgctg
     6601 aaggtcggaa tccattataa tacaagcatc gcctactgca gcttctaatc ctgcgcaaag
     6661 ggctgcttcc ttgccaaaat ttctgctgag ccgtaacgct ttaatgccgg gatgtgtttc
     6721 cgtgagcatt ttaaggacaa accacgtgtc gtccgtagag ccgtcgtcta taagcaggat
     6781 ttcaaaattc atttctgttt ttgatagata gtccctgatg atattaatat tttcaaaaat
     6841 ctggttttct tcgttgtata ctggaataat cactgtaata aatgacttac ccattttcag
     6901 ctaacctccg ttattaatat atctatatta tccataccta tgttgcctgt attgcatttt
     6961 atatttgaac catatgttat tgttgcaaac aaataatata attgttcttg cagcactcaa
     7021 acatcagaaa tgtcctactt tcacaacatc gaaacatgag aaccgtccat atgttctcca
     7081 tcttctgcat tttctgtatg ttcagaaaca ggagaatcgt ccctttgttc tgcaccttgc
     7141 aaaatatgag aaccgtctcc atgcatctca cgttttttcc atatgcttct tcccaggtat
     7201 cctaaggcaa gtattaaaat ggaagccaat gttatcatag aacctatgaa aaatttctcg
     7261 ggaatgaacc ataattcaat tttatttgaa cctgcgggca gttcaaaggc cagcaggcaa
     7321 tcgtctatag cctgtgtttc aactttctga ttattgacct tgacatgcca gcctttgttg
     7381 tatggtatgg tcataaccat cagtccatca gttgatgttt ctatgtgacc acggatcctg
     7441 gtatctttaa agctttcaat cgtcattgat ttttctctta taagcgacat tgcattctca
     7501 aattcctgct gattcaaacc gcagacatat atttcaaacc tgcccgattc cggagctgac
     7561 ttttcaaagg ttaattgaag ttcaggtaga gtaccggatt tgcagaatcc aaggttaata
     7621 attgaagaat gcttagggtt aaaatcaact tttttatcat caatcttaac aaaaccgctg
     7681 ccttccatat tgtaaggtgc tttgaaataa agataaacct gctgatcttc tttgactgaa
     7741 aatttaacct ttgcggtaga acttacttcc ttattggtcc gattatagct gtaatacctt
     7801 gtgccggaac tactgaaagt cagattatcc tgtgcccctt gttcctgatc tattgggaca
     7861 agaatatcct ttattccaga aatataatta ataagggcat tctgagcatc aaaaggattg
     7921 gtccatgtac tgtgaaattt tttcagttca tctggagctt ggaaacccaa tgtcaaggtg
     7981 tatggatttt tataaacagt catctcttca gtagctgcaa ccgtttcata taagtgttct
     8041 tcaatatttt tggagcggta aatcagatat ttgataccaa aaagagaatc cagtacagct
     8101 gtggatccct cgtactcata gctgttgatg ctattgctat ggtaacccag gttctcaaac
     8161 atttttacag gcttcacagg caaagtggaa gaaaaaatag agagaccccg gtaattataa
     8221 aggtatgggt cattagtagt tttgggcggc agtacctcca tacggtagaa atccttgtct
     8281 ttcttttcta tatcacgaat ccattcgcgg atttgtgcga cttccttacc acttgaatat
     8341 ccgtcacgga atgaataacc ttcaagtgaa tcaattttta tggtggttat aattgtactt
     8401 gacactaatt cagcagttat ggctagaaaa aagaacaggg ccctgggcaa atgtgaatgc
     8461 cggattttgt atgaacggtc aagtgcaaag gcaacagcat acaagatgat ggatattatg
     8521 ctagcatata taatagcata ttccagtgac aagttatcaa atttttgtga aataacaata
     8581 atacccagca ctgacatgca gatagctcct atctgtttgc tggtaaactc attcagcctt
     8641 ttgaaggcat catagcacat ggataagatc aggaagatgt aaacaaaaga aaaccggtat
     8701 ggaatctggt tgggataatg gaaaccatgc catatgaaat ttaaaatatt aatgttgaaa
     8761 cttacaagta gaactgcaag cagtgctaaa ttccataact tttctttcaa ccgaatattt
     8821 ttgcacagga aataaacagg taaaaggatt aaaacaacaa ttcccgaata caggttcggc
     8881 attccttcgc ggatggaagg cgtagcagcg gtaaaatgtc tggttatata atcaaacaga
     8941 tcaaaataat gtgtaatcgt cttgggaagc acatcatctg ctgcagaggt tgttttaagg
     9001 gcaaagtatg tgggcaggag cagcacagct gaaagtcctg cagacagcag tgaatatcct
     9061 gcaaatttcg cagttttttt gagcaacaga accggttttt tgatactgtt atattgaaag
     9121 agaaggactg gataataaag catagtaaat aaacatatga aaaatccaat gtagaaattc
     9181 gacaagagtg caacagccaa ggtaatgcag tagaaaaggc ccttggaatc acggaccaat
     9241 ttcacaagtc ccagcataat caacggcaag aggtaaattg catccatcca cattatattc
     9301 caggaaaaca ccagtacata gcctgaaagg gcataaagca gcgaaaaagc cactgtgacc
     9361 agactttgtt catgatggac acctttaagg taataggcaa aacaggctcc ggccaggcta
     9421 accttcaata gcgttaatac tagtactgct tcagccaaat attctttggg gaataagact
     9481 gtaatcagat taagagggct ggccacataa taagcatata atggcaggta gttggtacca
     9541 agaccgcctg accatgaata caacaagctc gaaaatgacc ttagtctatc ctgcaaatca
     9601 gatataaagg gagcatattg atgataaagg tctataataa gtaaatccca tttaccaaat
     9661 ggaaatatgc cacatgatat ataagccagc tctagtataa ttgccggaag aaaaaaagat
     9721 agaagaatta cgtaattttt tcttataagc aaacccgttc tttttataat atgtttgttg
     9781 tatctattgc atctcaaatt gttgtctatc aaattgttgt atctcaaatt gcttcctctt
     9841 ccttatagta tagtgatttc gcttttcttt ataaataaaa ttatagttaa atcttaaaat
     9901 actcagtttt aacagcacac tgctttttat gatgaccgat tcccataagc aggacgtctt
     9961 tgaagccttc tccttcaaaa accttgtagt actttctatc ttttatttgt tttattgctt
    10021 cataagctat ggtttccagg gctaaacttt cccgattatc tccactgtcc ggattacccc
    10081 agctgctcgg attatctccg ctgtccgtat tatctccatt gaccgtaggt ttttttgcag
    10141 ctgcaagctt ttctatgcct tcttttccca aagctttaaa ctccattata attggatgat
    10201 attttttatc ctttggaatt aggattatat cggatcttcc aagtccgact tctaaattag
    10261 atttaacagt atatatattt cttaatg
//
LOCUS       DAOSDD010000160        10246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2485_length_10246_cov_4.771563, whole genome shotgun sequence.
ACCESSION   DAOSDD010000160 DAOSDD010000000
VERSION     DAOSDD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_10246_cov_4.771563"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1048..1254)
                     /locus_tag="PK733_13305"
     CDS             complement(1048..1254)
                     /locus_tag="PK733_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOJ11552.1"
                     /translation="MEYFYTFCVLKESYIKAAGKGLSIPLNSFSFIIKNQNIQLTSRN
                     EFGPCSFWKQQIGKRHIIAVCAQI"
     gene            1748..2395
                     /locus_tag="PK733_13310"
     CDS             1748..2395
                     /locus_tag="PK733_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11553.1"
                     /translation="MKKLINILCVCFISFIVCSSSEQDTKLEGYLEGLMPVLSYEDML
                     QESELIAEVKILKKLSIAEIKKDPAYYNGWDGNSFFEALIVEDYKNTQMNGQKIVLKQ
                     LGNEKWRYENHPIFSVDEQFLLFLNKASVNNTISSIYYIVGFMDGTFDIEEYESKKYA
                     KNRGREFNSLDEIKVKNITGLKDTIKYSNKKYQSIFLLNDLKYKIKDTLKDGDKK"
     gene            2421..3290
                     /locus_tag="PK733_13315"
     CDS             2421..3290
                     /locus_tag="PK733_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11554.1"
                     /translation="MIIVFISIINFGSILVYGHSGDITVNYAEKIGWNIKEYEYHFSS
                     GNSSYKFDSSVIDRYKDYFRGGVDKWESCTFDGASIASFVEKSAQENAYIEMFQINDN
                     TKGVAYADNPNATVDGNNLYTYCPIRLNEYYMDKPEYKDTTGKNDYVCAHEIGHFFGL
                     KDLYDDNQDKLMWYTSASSSWSPSSADIKGMVVISGYYISRSHTFTESWSLGIEPDCE
                     TEAYRVYSCNNCIPVGYSYNVYYPPLGHLWTDDCDSVCDRSGCGATRTPPHVGVHLAM
                     NTVTCASLAEHQA"
     gene            3599..3934
                     /locus_tag="PK733_13320"
     CDS             3599..3934
                     /locus_tag="PK733_13320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ11555.1"
                     /translation="MGLVDVPSAVEKVKRLVNDNTLYIWKDEIPVSQAAMGRRTLNGA
                     VVNAVYTPPHYRGKGYATSCVASLNQHLLDQGYKFCSLFTDFANPVSNSIYMKIGYKP
                     VCDYDEYMF"
     gene            4312..4773
                     /locus_tag="PK733_13325"
     CDS             4312..4773
                     /locus_tag="PK733_13325"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ11556.1"
                     /translation="MSKLVSKITDSELKVMHVLWEAGCELPIADIRKTLEQTSKWEAS
                     TIKTLLRRLCQKGVVSATKKEVFYYKPLVSEEEYNEYATQSLIDRLYAGSAKNLVASL
                     LGSKKLDKSDIEELRTMFKVGDREVAGRKSGNRVVEAKKFIEAGEAGDRNE"
     gene            4766..7213
                     /locus_tag="PK733_13330"
     CDS             4766..7213
                     /locus_tag="PK733_13330"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ11557.1"
                     /translation="MNEIIKLILSLSLSGSILAVLIFAVKPVIKHRLTKSIQYYIWIV
                     VLLRFLLPFSFEGSIMNKIFYGDQSSWTMSNQTIVQPMNGTSDNKNNTDNSSDFSDSH
                     ALSTLPSSVLPNTHERTANGVYNNDVDHGKYFKDLFFRYALYLWLIGAILAITNNLAG
                     YARFLRHLKQTNKPATEEQNIIFNVLLRDKNNTGINIGNNVRLVCNRFVTTPMLVGIL
                     KPCIIIPDIDFDDKQLENILLHEITHSRHFDIVIKWLTMIAASIHWFNPLIYLIKKEI
                     NHACELACDETVIKNLSLTEKQAYGDTLISVVAEHKYPVGVLQATMCEEKKSLKERLI
                     AIMNHNKKSKYMVIFAVILLGIVTLSALYLGAGIGTGKDTPYNIYISAENSETKAAQI
                     GTYSWSYRGTHIEADSDHPMNFEYRPDNIIAVEPEQQLIISTQKIKSDRKYNFSIEQI
                     SVYKDKQMIQFESVEPSLINGNLYIQAPPDVGEYIYTMILNFKDKGSVNYGFVVRAGM
                     LTYDLDGIAKYKTPYIGDASKVGSIVSHLPVPDSYFKQQYISMETSKKPYGLTIYYEA
                     ASNSEYNKYKDVWPITASNSVITTNLRANALVTFCLIDNLDEVAFAFRNSQSGGKLDK
                     SKYDSSFVYTRAGFEEEYGDLSALAENLDLLKEILTEKKTVLKGKELANDLPMPEFSD
                     EEVAAALTVVEEYFRAIAAKDDETILATMYPREHYTIERVKSGNVQLYGAEIRTLLTI
                     DYDSQDNIRRIYRPGNHQISEENIIVFRVSFKIQYPLKDGIKVEGPWHEGIYDDWNII
                     LIRDNQDSPWLIYDQGY"
     gene            7348..8031
                     /locus_tag="PK733_13335"
     CDS             7348..8031
                     /locus_tag="PK733_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOJ11558.1"
                     /translation="MIKRKAKKKLSNALLVLMLVSILVLASILIIDAYVKSSVKDKII
                     TSDEASSLDSDCILVLGAGVWNNGRPSPMLEDRLLQGIELYENGASDRLLMSGDHGRK
                     EYDEVNAMKRFAIDKNIPSEHIFMDHAGFSTYESMYRARDIFQAHKIIIVTQKYHLYR
                     AMYIAEKLGLDAYGVASDPRQYVGQEYRELREVLARVKDFFKVIIKPEPTYLGQTIPV
                     SGNGDLTND"
     gene            complement(8103..8252)
                     /locus_tag="PK733_13340"
     CDS             complement(8103..8252)
                     /locus_tag="PK733_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11559.1"
                     /translation="MGILFMNLMSYKLLLREYLQHNNYREAKMADFIEWIQLRNIHST
                     SEYLN"
     gene            8417..9211
                     /locus_tag="PK733_13345"
     CDS             8417..9211
                     /locus_tag="PK733_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393332.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOJ11560.1"
                     /translation="MIFYFSGTGNSLQVARNIAEHAGEKLISIAHAINMDDCCEYTLK
                     HDEVIGFVYPVYAWSPPKIVLQFIEELKLNNYNDNYIFSIATCGDNIGNTMKVLEDTL
                     SKKNLTLNSGFSVNMPNNYIIFFDVDSMELEKKKLSAAERTLEKINAVIAEKKKGVFE
                     IEKGLFPSILTNVINPFFNKKAINTKKFYANEECTGCGLCEKVCNSRNINIGADRKPQ
                     WGRQCLQCMACLHYCPVEAIQYGKRTVHKGRYTNPYVSPEDISLEK"
     gene            9760..10062
                     /locus_tag="PK733_13350"
     CDS             9760..10062
                     /locus_tag="PK733_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HOJ11561.1"
                     /translation="MKYKNAKAVLPERLYRELQQYVQGEIIYVPKDDSEKAGWGEKNG
                     TKEKYIDRNTEIIMLYKSGVSKEAIADRYHLSECSIKKIIHSSKHKACGLEEVSNS"
BASE COUNT         3576 a         1445 c         1930 g         3295 t
ORIGIN      
        1 cagacgcaat atcgttcata taggataatt cctttcttat gatagcatat tagtgcatat
       61 ttgtcccatt ccttattttt cacttgtgta tcttgtgtat attatatcat gaatttcgaa
      121 gaaattgcat gatataattg tgcatgctta ttttttataa atcgtaactt taaatgccgt
      181 acatttaatg gaagaaggag gtaacaatag taattaaaat cattaacaaa tgtgtttata
      241 tgtgtacaaa cacacttaaa tgaaatttat gaatagtaaa aaatacacac aaaacacaaa
      301 taactgaact attttggatt ataaattgat atatggtgcg cattcaaaac aaagcttata
      361 aaaatttata tttttcaatt aatcattgca ttccgaacag ttgtttggta taatttaact
      421 atgaacataa gttcggggga gcgttgattg aagaatataa aatgtaattt ggggttctat
      481 gcgcaaaccc ccgaaatttt tgatagcgta aaatcatccc taaatgtttc aaaaatcact
      541 gtttatgaac acaaaaggat aaaggggtat cgacaatatt aaaaatctaa gactaagaca
      601 atataaattt gatcacggct acctacgatt ctggctctag cattgatatt ggttctatca
      661 agaactccgt gttttcctcc aatcatatta tggccagctc agagttttcc tacattcgcc
      721 atcagtttat caataatagc tagtccgagt ttttggcgaa actttaccaa cgtagttctg
      781 tcccggaggg tttaatccaa accaatatca agaaagtttc ggaaacatgc ccggtcactt
      841 atctccgcca agagttcatc atcacctgca aggaataatt tagttttaaa aaactccgct
      901 taagtagggg tagtgggagc atcccaaggg caaagtatat ggtattttat gggttgtaca
      961 atttgttttt ttcggtttaa tagttaacaa attatgcaac aaccatatta taccattaaa
     1021 aacaataatt aattttatat atgaaactca tatttgagca cacaccgcaa ttatatgtct
     1081 tttaccaatt tgctgtttcc aaaaagaaca aggaccaaat tcgtttctgg aagttaactg
     1141 tatattttga ttttttatta tgaatgagaa tgagttcaag gggattgata agccctttcc
     1201 ggctgcttta atatagcttt ccttcaatac acaaaatgta tagaagtatt ctatttttca
     1261 ctcttatctt tatccattaa ggattatagg ttaaatacct ttactgttac attaaagagc
     1321 gcaaaaccaa aaaattgtac tttcctcact tttctggtat acaaaaataa aaatctatgg
     1381 cgtttgaggg taaaaccttc aattatttgt tttgtggaat tgtcaaagaa ctgcaaagtt
     1441 tttatttaat cttagtgttt aattgtatag tcaaaattgt agcaattatg taattatatt
     1501 gtaataaaca aaaaaaggaa tttaatactg atacatagaa taattacaat ggattaatta
     1561 tcatataatg gtaataatga aatatatgat atttatttgg aggttttgtt attatgatat
     1621 atataagatc cccaagcata attaatactt ctagttatta attgaattaa ttagtcaatt
     1681 ttgatatttg cataaaatgt gttagcttaa taaatttttt atattatatg agataggaat
     1741 ggtttatttg aagaaattaa ttaatatact gtgtgtttgt tttatttctt tcattgtttg
     1801 ctcatctagt gaacaagata ctaaattaga aggttatctt gagggtttga tgccagttct
     1861 tagttatgaa gatatgttac aggaatccga gttaatagct gaagtcaaaa tattaaaaaa
     1921 gttgtccatt gcagaaatca aaaaagatcc agcttattat aatgggtggg atggaaattc
     1981 tttttttgaa gcattaatag tcgaagatta taagaacact caaatgaacg gacagaaaat
     2041 agtgctgaag caattaggaa atgaaaaatg gcgttatgaa aatcatccga ttttttcagt
     2101 tgatgaacaa ttccttcttt tcctaaataa agcatctgtt aataatacca tttccagtat
     2161 ttattatata gttggattta tggatggaac ttttgacata gaagaatatg aatctaaaaa
     2221 atacgctaaa aatcgtggaa gagagtttaa tagccttgat gaaattaaag ttaaaaatat
     2281 aactggttta aaagatacaa ttaaatactc taataaaaag tatcaaagta tatttttact
     2341 aaatgattta aaatacaaaa ttaaagatac tcttaaggat ggtgataaga aatgaaaaaa
     2401 aataccgcag aagtatttta ttgataatag tttttatatc aataataaac tttggatcta
     2461 ttttggttta tggacattct ggagatatta ctgttaatta cgctgagaaa attgggtgga
     2521 atattaaaga gtatgaatat catttttctt caggaaattc gagttataag tttgatagta
     2581 gtgttattga caggtataaa gattatttca ggggaggagt tgataaatgg gaatcatgta
     2641 catttgatgg agcatcgata gcatcatttg ttgaaaaatc tgcccaagag aatgcatata
     2701 ttgaaatgtt tcaaataaat gacaatacta aaggtgttgc atatgcagat aacccaaatg
     2761 ctactgtaga tggcaataac ctttatacat attgtccaat caggttaaat gagtattata
     2821 tggataaacc tgagtataag gacacaacag gaaaaaatga ttatgtatgt gcccatgaaa
     2881 taggacattt ttttggatta aaggatcttt atgatgataa ccaagataaa ttaatgtggt
     2941 atacatccgc atcttcatca tggtcacctt catcagctga tataaaaggg atggttgtta
     3001 ttagtggata ttatataagc aggagccaca catttactga atcttggtca cttggaatag
     3061 aaccagactg tgaaacagaa gcataccgag tttattcgtg taataattgt attcctgttg
     3121 gatatagtta taatgtttat tatcctccat taggtcattt atggacagat gactgtgata
     3181 gcgtttgtga tagaagcggt tgtggtgcaa ctagaacccc acctcacgtt ggagttcatc
     3241 ttgcgatgaa tactgtgact tgtgcttcac tagcagaaca ccaggcgtag gacatacata
     3301 cagtcctgat tactatagcc caatacaccc ccatcgtcgt tggtatattt gtgattattg
     3361 tgatgatatg catttatcaa cagaagagta tccttggggt acttattgga ctgattggta
     3421 cgtaatattg tcttagtctt agatttataa tattgttgat actcctttat ccttttgtgt
     3481 ttcattaaaa aacagtgatt tttgaaacat ccagggatga ttttacgttg tcaaaaattt
     3541 cggggggttt gcgcatagaa ccccttattg gaatatatca tttacttccg actgcggaat
     3601 gggtctggtt gatgtaccaa gtgctgtaga aaaagtgaaa aggttagtaa acgataatac
     3661 tctatatatc tggaaagatg agattcctgt ttctcaagca gcaatgggta gaagaacgct
     3721 taatggtgct gttgtgaatg cagtctatac tccgccgcac taccggggaa aaggttatgc
     3781 tacttcttgt gtagcaagtt taaaccagca tcttcttgat caaggttata agttctgcag
     3841 tttgtttaca gattttgcaa acccggtttc taatagtatt tatatgaaaa tcggatataa
     3901 gcctgtgtgt gattatgacg agtatatgtt ctaggggata ttagagcctt atttatcaag
     3961 gatgactata aaaaggaaat ttttgttttt gacctcatct caagaggtga tatttacgag
     4021 attataggta aaaaaagtag cgaatcaggt gcttcattag aaatattata ttggaaggtt
     4081 ggtatcaact gttatgagta gtttattttc agagtatata gttaaaggta aaaccatcaa
     4141 aaacagagtt gtcatgcctc cgatggtttg cttcggctgg accaatcata caggctttgt
     4201 cactgaaaat catttgaaac actatgagtc ttaaaaactc cctattgaca tttctgggtt
     4261 acggatgtaa actatataca gactacacat gtaacctgga ggggaattta tatgagtaaa
     4321 ttagtatcaa agataacaga ctcagaactt aaagtcatgc atgtattatg ggaagctggc
     4381 tgtgaacttc ctattgcgga tattagaaag acacttgaac agaccagcaa atgggaggct
     4441 tcaacaataa aaactttact tcgcagactt tgccaaaaag gagtagtttc agcgactaaa
     4501 aaagaagtgt tctattataa gccgttagtt agtgaggaag aatacaatga atatgctaca
     4561 caaagcttaa ttgaccgtct atacgccggt agtgctaaaa atctggtggc atctctttta
     4621 ggaagcaaaa aacttgataa atcagatatt gaagagctgc gtactatgtt taaagtaggt
     4681 gacagggaag tagcaggcag gaaatccggt aatagggtag tagaagctaa gaaatttata
     4741 gaagctggag aagcaggtga tagaaatgaa tgaaataatt aagctaatac tatcactttc
     4801 actatcaggc agcattctgg cagtactgat atttgctgtt aagcctgtta taaagcacag
     4861 actaacaaag tctatacaat actatatctg gatagtggta ttactaagat ttttactccc
     4921 tttttccttt gaaggaagca tcatgaataa aatattttat ggtgatcaat catcgtggac
     4981 aatgagcaat caaacaatag ttcaaccaat gaacggcaca agcgataaca aaaacaatac
     5041 agataattcg tctgattttt ctgattcaca tgcgttatct acattgccat catctgtatt
     5101 gcccaatact catgaaagaa cagctaatgg tgtatataat aatgatgttg accacggtaa
     5161 atatttcaag gatttgtttt tccggtatgc tttatatctt tggttaattg gtgcaatctt
     5221 agcaattaca aataatttag caggatatgc tcgattttta agacacctga agcaaacaaa
     5281 taagccagcc actgaggaac aaaacattat ttttaatgta ttattaagag ataaaaataa
     5341 taccggaata aatataggaa ataatgtaag acttgtatgc aaccgcttcg tgactacccc
     5401 aatgcttgtg ggaatactaa agccttgcat tataattcca gacattgact ttgatgataa
     5461 acagcttgag aatatactgc ttcatgaaat tacacattca agacattttg atatagtaat
     5521 aaaatggcta acaatgatag ctgcctcaat tcattggttt aatccactta tttacctgat
     5581 taaaaaggaa attaatcatg cctgcgaatt agcttgcgat gaaacggtta ttaaaaacct
     5641 tagtcttact gaaaaacaag cttatggtga cacattgatt tctgttgtag cagagcacaa
     5701 ataccctgtt ggagtattgc aggcaactat gtgtgaagag aaaaaaagtt tgaaggaaag
     5761 attaattgca atcatgaacc ataataagaa atcaaagtat atggtaatat ttgctgtaat
     5821 tctgctaggg attgttacat taagtgcatt gtatttaggt gcaggaattg gaactggtaa
     5881 agatactcca tacaacatat atataagcgc agaaaatagc gaaacaaaag ctgctcaaat
     5941 tggaacttat agttggtcct atcgtggtac acatattgag gcagattctg atcatcctat
     6001 gaatttcgag tatagacctg acaacattat tgctgtagaa cccgaacagc agctaataat
     6061 tagcacacag aaaataaaat cagatagaaa atacaacttt tcaatagagc aaatttctgt
     6121 atataaagac aaacagatga tccaatttga atcagtggaa ccaagcctta taaatggtaa
     6181 tttgtatata caggcaccac cggatgtggg cgaatatatt tatactatga tactaaactt
     6241 taaagataag gggagtgtga actatgggtt cgttgtaagg gcaggtatgt tgacatatga
     6301 tttggatggg attgcgaaat ataaaacacc atatataggg gatgccagta aagtcggatc
     6361 tattgtcagt catctgcctg ttcctgacag ctacttcaaa caacaatata tatctatgga
     6421 aaccagtaaa aaaccttatg gccttactat atattatgaa gctgcatcaa attcggaata
     6481 taataaatac aaagatgtgt ggccaataac tgcgtcaaat tctgttatta caacaaattt
     6541 gagagcaaat gctttagtca cattttgcct gattgataat cttgatgagg tagcctttgc
     6601 attccgcaat tcccaatctg gtgggaaact tgataaatca aaatatgata gttcatttgt
     6661 gtacactaga gctggatttg aagaggaata tggtgatctt tctgcacttg cagaaaattt
     6721 ggatttgctt aaagaaatat taacagagaa gaagactgtg ttaaaaggga aagagttagc
     6781 gaatgatctg ccaatgccag aattttccga tgaagaggtt gcggcagccc ttacagttgt
     6841 tgaggaatac ttccgtgcta tcgctgcaaa agatgatgaa accatacttg ctacaatgta
     6901 tccacgtgag cattatacaa ttgagagagt aaaaagtggc aatgttcagc tttatggtgc
     6961 tgagatacgg actttattaa caatagatta tgattctcag gataatatac gccgaatcta
     7021 tcgtccgggc aaccatcaaa tatctgaaga aaatattatt gtttttagag tcagcttcaa
     7081 aatccaatat cctttaaaag atggcataaa ggttgaaggc ccgtggcacg aaggtattta
     7141 tgatgattgg aacatcatac tgatccgtga caatcaagat agtccgtggc ttatttacga
     7201 tcagggatat tgataaccct gaaatgagag ttaaatttga cattctttat ttgcttaatt
     7261 ataataaatt caggaaaagc taagggaaat atccatgatg tttattaaaa aatcaacatt
     7321 atttacttta catggattgg tgacaatatg ataaaaagaa aagcaaaaaa gaaactgtca
     7381 aatgcattac ttgtattaat gttagttagt atattagtat tggcatctat attaattata
     7441 gatgcttatg ttaaatcatc ggttaaggat aaaattatta catcagatga ggcaagttca
     7501 ttagattctg attgcattct tgttcttggc gcaggtgtat ggaacaatgg aagacctagt
     7561 cctatgcttg aagataggct tttacaaggc attgagttat atgagaatgg ggcttcagac
     7621 agattattga tgagtggtga ccatggtcgt aaagaatatg atgaagttaa tgctatgaag
     7681 cgatttgcga ttgataagaa tattccttca gagcatattt ttatggatca tgcgggtttt
     7741 tctacatatg aaagcatgta tcgtgcaagg gacatttttc aagcccataa gattataatc
     7801 gtgacacaga agtatcattt gtatcgtgcg atgtatattg ctgaaaagct tggacttgat
     7861 gcgtatggtg ttgcctctga tccaaggcaa tatgttggtc aagaatatag agaattgaga
     7921 gaagtgctgg caagagtaaa agatttcttt aaggttataa ttaagcctga gcctacttat
     7981 cttggacaaa ccatcccagt aagtggaaat ggcgatttaa caaatgatta gctgctttag
     8041 cagtgcggct attcaaagtg tcatttatta caatattact gcgcaatact tccttttatg
     8101 atttaattca agtattcgct tgtactatga atgtttctca attgaatcca ttctataaag
     8161 tcagccattt ttgcctctct atagttgtta tgctgtaagt attctctaag aagtaatttg
     8221 tatgacatga gattcataaa taatataccc actttattac cacctttcaa actctataaa
     8281 aactattgcc attattaaga atattattca tcttgtctaa atattacttg attagatcat
     8341 gaaaaaatag taaattggaa gtaacaagct agttattgtt aaataactta tgaaaaagct
     8401 ataaggggtg aatatcatga ttttctattt ttcaggaact ggaaattcgt tgcaggttgc
     8461 aaggaatatt gcagaacatg caggtgaaaa actaatatct attgctcatg caattaatat
     8521 ggatgactgc tgcgagtata cgcttaaaca tgatgaagtg ataggatttg tgtatccggt
     8581 atatgcatgg tctccaccca aaatagttct tcagttcatt gaggaactaa agcttaacaa
     8641 ctataatgat aactatatat tttcgatagc aacgtgtggt gacaatatcg gaaatacaat
     8701 gaaagttctg gaagataccc tcagcaaaaa aaatttgact ttaaacagcg gcttttctgt
     8761 taatatgcct aacaactata ttatcttttt tgatgtggat tcaatggaat tggaaaagaa
     8821 aaaactttca gcagcagaaa gaacgctgga aaagattaat gcagtaattg ctgaaaagaa
     8881 aaaaggtgtt tttgaaattg aaaaaggact tttcccaagc atattaacca atgtgatcaa
     8941 tccatttttt aacaaaaaag ctataaatac aaagaagttc tatgcaaatg aggagtgtac
     9001 aggctgtggc ttgtgtgaaa aggtatgtaa tagcagaaat ataaatatcg gtgctgatag
     9061 aaagccacag tggggaagac agtgtttaca atgtatggct tgtcttcact attgtccggt
     9121 tgaagccatt caatacggta aaaggacggt acacaagggg agatatacaa atccttatgt
     9181 atcaccagag gatatttctt tagagaaata ataataaata aataatgggc tgtttaaaaa
     9241 acaattataa ttattctttc tcttgacgga ttatattctt gactataaaa taaaattgca
     9301 ttatataata tttcagtaag aagggagtgc ttttttatat gaaaataaca agtgttgagg
     9361 tgaccctctt ataactgaat atttgtaaaa gacacaaggt ttttatagga tgatttcgag
     9421 gtttctatgt attatcaact ataaaaataa ataaatcttt aatgaggatt tacttgttgt
     9481 gtcttttgca gcaacttttt gtaataaccc tttagcctga atataatatt tggaaatgtc
     9541 tctttctgtt tcattacaac aaacggcacg gtaaaggtta tcgtgttttt tttatgtcca
     9601 caggtggtta ttatctgtgg attttttatg tctgaaaatg gaaagtgcag aacgaaagtg
     9661 gaaaacatgg aattaacagc atagaattaa ttttgacagg cagcaaaatt ttggatgaaa
     9721 agtgttctct tatgacttat gattggagtg tggatcttat tgaaatataa gaatgcgaaa
     9781 gcagttcttc ctgagcgact ctacagagaa ctgcaacaat atgttcaagg tgagataatt
     9841 tatgttccca aagatgattc ggaaaaggcc ggatggggtg aaaaaaatgg tacaaaagaa
     9901 aagtatattg atagaaatac ggagattatt atgctttata aaagcggtgt tagcaaagaa
     9961 gcaattgctg acagatatca cctctcagaa tgtagcataa aaaagataat tcatagcagc
    10021 aaacataaag catgtgggct tgaagaggta agcaacagtt agatagaagt ttgcagtgat
    10081 tcaatacggt ggataaaaga atttgttgat gaaaaaaact tcctgaatac tttgattaaa
    10141 gatgatatct gctgtataat atataaaata gtattaaatt taattctact tgataactcg
    10201 tgatggaaaa acagagtagt cgaagtagtt gaaaggagtg atttct
//
LOCUS       DAOSDD010000161        10190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2501_length_10190_cov_4.762802, whole genome shotgun sequence.
ACCESSION   DAOSDD010000161 DAOSDD010000000
VERSION     DAOSDD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2501_length_10190_cov_4.762802"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PK733_13355"
     CDS             complement(<1..1187)
                     /locus_tag="PK733_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ11562.1"
                     /translation="MLKDCIKPAKITGTAREDRENKRFKKTLTWMVIYSTLLLITTGC
                     SFSRGKASLTAPSLQEPPEIKFYTFDVQKGDIKKEIKDAAKIVPSTETLIFSHFGGWL
                     SEICVKSGDIIHKGDILVKFDTDSLEIKLKQQELALEKAVLNSNRLMEDIQRDIDLAT
                     LDLKNLEEKLNKSKQLRQELSKNNSASDLKQIDEEIENLQGSIIRQKMSLEGLNEKYE
                     ATKASLDIDMKKSELQIKNVRKELENAIFVSPIDGKITNLTNKAAGEYIGAYQTLASI
                     VKIEDLHLVYSGSNVNSFEVGMKVKVILDKEELDGEVVSSPAYYGKNVPGNIKESVII
                     NIKGLTMEHLTNLKGDVRINLVLEEKNDVVVVPKRVLNNHLGNKYVYVVENGIKKKRF
                     VETG"
     gene            complement(1273..1932)
                     /locus_tag="PK733_13360"
     CDS             complement(1273..1932)
                     /locus_tag="PK733_13360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOJ11563.1"
                     /translation="MALINVNPTRMELTRLKKRLVVARRGHKLLKDKRDELMKKFLDL
                     VRKNKELREKVEEMTMDVHSSFLIARSVMPSEVLEEALMFPKQTVSLEAGSKNIMSVD
                     VPVLSFHTSSNDQGDIYPYGFATTSGELDSAITALSKVMPYLLELAEVEKSAQLLAEE
                     IEKTRRRVNALEYVLIPQLTETIKYITMKLDENERGNLTRLMKVKDMMLEQARQENPW
                     S"
     gene            complement(1972..3354)
                     /locus_tag="PK733_13365"
     CDS             complement(1972..3354)
                     /locus_tag="PK733_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ11564.1"
                     /translation="MLKEYKTISEVAGPLMLVKSVEGVKYGELGEIELSSGEVRRCKV
                     LEVNKDKALVQLFESPAGINVAESKVRFLGKGLELPVSVDMLGRVFSGMGEPIDDGPN
                     IIPDMRLDINGVPMNPAARDYPSEFIQTGISAIDGLNTLVRGQKLPIFSGSGLPHAQL
                     AAQIARQAKVLGTESKFAVVFGAMGITFEEANFFITDFRKTGAIDRAVMFINLANDPA
                     IERISTPRMALTAAEYLAFQKDMHVLVILTDITNYAEALREVSAARKEVPGRRGYPGY
                     LYTDLATLYERAGRKRDNKGSITLIPILSMPEDDKTHPIPDLTGYITEGQIILGRELH
                     RKGIEPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYARGKEAKELAIILGEA
                     ALTDVDKLYAKFADTFEKEYVSQGFDEERPIEQTLEIGWKVLSILPAGELKRIRDEYI
                     KKYLNKIDGK"
     gene            complement(3347..5125)
                     /locus_tag="PK733_13370"
     CDS             complement(3347..5125)
                     /locus_tag="PK733_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ11565.1"
                     /translation="MGQGRIVKVSGPLVIAEGMRNANLFDVVRVSEQRLIGEIIEMHG
                     EEASIQVYEETAGLGPGEPVETTGFPLSVELGPGLIGKIFDGIQRPLEVIREMVGSNI
                     TRGIEVSALDRKEKWHFKPVIGKGARVEAGDIIGVVQETVIVEHRIMIPYGIEGIIEE
                     IYEGEYSIEDTVAKIKKDNGEIANIKMIQKWPVRKGRPYKEKLPPDTPLITGQRVIDT
                     LFPLAKGGTAAVPGPFGSGKTVVQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMKRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERSGRVISLGTNGREGALTAIGAVSP
                     PGGDLSEPVTQATLRIVKVFWGLDSGLAYRRHFPAINWLNSYSLYLDRLDKWMEQNIS
                     REWSVLRSDVMRVLQEESELEEIVRLVGIDALSSADRLTLEAARSIREDYLHQNAFHE
                     IDTYTSLNKQFGMLKLIMTFYYKGKKALESGVAIKDLFAIPVRERIGRAKYTHEDEVN
                     AVFFDIETELNRQIDGLISKEVSFGA"
     gene            complement(5176..5490)
                     /locus_tag="PK733_13375"
     CDS             complement(5176..5490)
                     /locus_tag="PK733_13375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOJ11566.1"
                     /translation="MYKIGVIGDKDSVLGFKILGLSVFPVLSPEEAVKVLHRLAGEDY
                     AVIYITEQVAEKISASIDSYKSSKLPAIIPIPGNQGSLGIGVRGIKKSLEKAVGADIL
                     FG"
     gene            complement(5483..6493)
                     /locus_tag="PK733_13380"
     CDS             complement(5483..6493)
                     /locus_tag="PK733_13380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOJ11567.1"
                     /translation="MPVDTRFAYAYAIGRIRVIEKKLLDKGKLDRMIDSKSPDEALKV
                     IVESGYVESSTLAGNAGEYENLLREEEKRLYGLLREVTSDDELFNIFLLKNDYHNIKV
                     LLKSEFLAIEANDNLLVSGTMPPMHIKTAINERELDRLPLYMKEAIDECLDTFSRTND
                     PRQIDLILDKAYFRHIVEISEKSGHEYVKELVKIIIDLTNIKTFIRIKNLNMSADLLS
                     RSLIPGGTIADSFFTENMDTNMEVFVEKIKNSPYFNICSQGISNYVNTGNLSRLEKLA
                     DDYIINFVKKSRYKTLGIEPLIGYLVAKENEIKNVRIIMVGKINNISGDIIRERLRDT
                     YV"
     gene            complement(6573..7172)
                     /locus_tag="PK733_13385"
     CDS             complement(6573..7172)
                     /locus_tag="PK733_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOJ11568.1"
                     /translation="MDGAEKLIEKIMEDARMQAMQNIEQAEKEADSVIKSAKEEALRK
                     RGSIIEKTEKEVSEKKKRLIAVAELEGRKIKLKAKQEVIAEAFLKAHEKLNLLPQEQY
                     KSMLADMIINMVPEGNEEVIVSKEDKQRLGNDFINLVNKRLKNMKKQGNIKFSEECRN
                     IGGGFILKSGNIETNNSFDTIFRMKYNDIESEIIRILFN"
     gene            complement(7195..7686)
                     /locus_tag="PK733_13390"
     CDS             complement(7195..7686)
                     /locus_tag="PK733_13390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOJ11569.1"
                     /translation="MKLLEVLLNGNFLALIGAACAVIFAGLGSARAVGMVGEVAAGVI
                     TEEPEKFAQTLLLQALPGTQGIYGLLTAFVILNKIGIVGGPVKELTTVQGVLFLVAAL
                     PIAIVGYKSAIAQGKTAAAGIGIIAKRPQELAKAVTFAAMVETYAVLALLGSILMVFG
                     INI"
     gene            complement(7689..9656)
                     /locus_tag="PK733_13395"
     CDS             complement(7689..9656)
                     /locus_tag="PK733_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOJ11570.1"
                     /translation="MSIVKMHKITLIGLEKEKSGIIESLMNIGVLDINDIGDTALTEE
                     WTGLVDKDNNEDEALTLELDMEKVRSALEYLNQYDKQKKPLFAPKRSVDALRFSDILK
                     NKDDMWVAVEKISKLDEELAQLRMEENRLENLIASLTPWKSMNTPVELISTKTTSVIM
                     GVLPIAADVKEIFKELSVNIPESYLQLVNVDKDQSYIQAICHKSREEELTRVLKNKGF
                     NRLSFTDMKGTINQNIENSLKSIEEVDRKRETLRGEIAALAGKREDLEVLYDYLAIQR
                     DRNIALGRLLKTECTFILEGWVPEHNINSFRNYISKWEWDCFVSIEESNKDEDFPILL
                     ENNRFVQPFELVTELYSLPKPSEIDPNPFMAPFFFVFFGLMVGDAAYGLLMAIATGIV
                     IYKFKPQGTVGKLLKLLFLGGISTFIWGALFGGWFGDIVTQVSSGKFTIPPLWFNPLD
                     DPMRLLMWSFIFGGIQLYTAMGLKGYSLIREGKVLDAVFDIGLWYIFLTGLVLLLVGG
                     NVGTIGKYMTIVGVILLILTQGRAEKNIFKKLTKGVLSLYNAVGFMSDVLSYSRLLAL
                     GLATGVIASVINTIGTLLGFNILGIIVLLIVFTGGHVFNILINALGAFVHSSRLQYVE
                     FFGKFYEGGGKPYKPFKINTKYINITDGRKV"
     gene            complement(9643..9969)
                     /locus_tag="PK733_13400"
     CDS             complement(9643..9969)
                     /locus_tag="PK733_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11571.1"
                     /translation="MTIEIVRSIKNAEQEADNLIKESISKAHNIMSDAEKQKNEVLAK
                     IVEEAEQESANLLMNMEKEVQGEIEGIYRKAEEEGKLIKNKARTNINKAVDIVVERIV
                     NSHVNS"
BASE COUNT         2889 a         2278 c         1425 g         3598 t
ORIGIN      
        1 cctgtttcca caaatcgttt cttctttata ccgttttcta ctacatatac atatttattg
       61 cccaaatggt tattaagaac tcttttaggt acaactacca catcattttt ttcctctaga
      121 acgagattta ttctaacatc tccttttaga ttggtaagat gctccattgt caatccctta
      181 atatttataa ttaccgattc cttaatattg ccaggtacat tctttccgta ataagcggga
      241 gaagaaacaa cctctccatc aagttcttcc ttatcaagaa tcactttaac tttcatcccc
      301 acttcaaaac tattaacatt tgaccctgag tatactaaat gaagatcttc aatttttact
      361 attgaagcaa gtgtctgata tgctcctata tattcacctg ctgctttatt tgtcaaattt
      421 gtaatctttc catctatagg ggatacaaat atggcatttt caagttcttt gcgtacattt
      481 ttaatttgaa gctcactttt tttcatatca atatctagac tggccttagt tgcttcatac
      541 ttttcgttca agccttcaag actcattttt tgacgtataa tactaccttg gaggttttca
      601 atttcttcat ctatttgttt taaatcactc gcactattat ttttagatag ttcctgtctc
      661 aattgttttg atttattcaa cttttcttcc aaatttttaa gatccagcgt tgctaaatca
      721 atgtcccgtt gaatatcttc cataaggcgg ttactgttta atacagcctt ttcaagggct
      781 aattcctgtt gtttgagttt aatttccagg ctatcagtat caaacttcac caatatatcg
      841 cctttatgta ttatatcccc tgattttaca caaatttcgc taagccatcc tccaaaatgt
      901 gagaatatga gtgtttcagt agaaggtaca atttttgctg catccttaat ttccttttta
      961 atatcacctt tttgaacatc aaaagtatag aattttattt ccggaggttc ttgaagggaa
     1021 ggtgctgtta acgaagcttt tcccctggaa aatgaacacc cggtagttat tagcagtaaa
     1081 gtgctataaa taaccatcca tgttaaagtt ttcttaaatc ttttattttc cctgtcttct
     1141 ctggcggttc ctgttatttt tgcaggttta atacaatcct ttagcatcag tttacacctc
     1201 accaaataac tcatgtttta ccttaacaca ctaatttgct ccttgtaaaa tcatttttgc
     1261 gttatatata gtttaagacc atgggttttc ctgtcttgcc tgctccagca tcatgtcttt
     1321 aactttcatt aatcttgtta aatttcctct ttcattttcg tctaatttca ttgttatata
     1381 ttttattgtt tctgttagtt gaggtattaa tacatattcc aaagcattaa ctcgtcttct
     1441 tgttttttca atttcctccg ccagtagttg ggcggatttc tctacttccg ccaactccag
     1501 caaataaggc attacttttg aaagcgctgt tatagcacta tccaattctc cggatgtagt
     1561 cgcaaaacca taagggtaaa tatctccctg atcattgctt gaagtgtgaa aactcaaaac
     1621 cggtacatca acactcatta tgtttttaga tcctgcttca agagacacag tctgttttgg
     1681 aaacattaaa gcttcttcaa gtacttctga tggcataacc gatcttgcaa taaggaaact
     1741 tgaatgcaca tccattgtca tttcttcaac tttttcccta agctccttat tttttctaac
     1801 caaatcaagg aatttcttca tcaactcgtc tctcttatct ttaagaagct tgtgtcccct
     1861 tctagcaaca actaatcttt tcttaagtct tgttaactcc atccgggtcg ggttaacatt
     1921 aatcaatgcc atgttagcac tcccttttaa atacaatatt taaagtaatc gtcattttcc
     1981 atcaatctta ttcagatatt ttttaatata ttcatctctt atacgtttca attctcctgc
     2041 aggaagaatt gaaagaactt tccatccaat ctcaagagtt tgctcaatag gcctttcttc
     2101 atcgaatccc tgtgaaacat attctttttc aaaggtatca gcaaattttg cataaagctt
     2161 atcaacatct gttagggcag cttctccaag aataattgca agttcctttg cttctttacc
     2221 tctggcatac gctgcaaaca actgattcat tgtatctgca tggtcttccc ttgttttccc
     2281 tttgccaatt cctttatctt ttaaacgaga taatgaaggt agaacatcaa taggaggttc
     2341 aatacccttt ctatgcaatt cccttccaag tataatctgt ccctcggtta tatatccggt
     2401 aagatctggt ataggatgag ttttatcatc ctcaggcatt gaaagtattg gtattagagt
     2461 aatacttcct ttattatccc tctttctccc tgctctttca taaagtgtag caaggtctgt
     2521 ataaagataa ccagggtaac ctcttctgcc aggtacttct tttcttgcag cggatacttc
     2581 ccttagtgct tcagcataat ttgttatatc agtaagtata accaatacat gcatatcttt
     2641 ctggaaagca agatattcag cagctgtaag agccatacgc ggtgtagata ttctttctat
     2701 tgccgggtca tttgctaggt ttataaacat tacggcacgg tcaatggctc ctgtctttct
     2761 aaaatcagta ataaagaagt ttgcttcctc aaaagtaata cccatggctc caaacacaac
     2821 agcaaacttg ctttctgtac ccagaacttt agcctgcctt gctatttgtg ctgccagctg
     2881 ggcatgagga agtccagaac ctgaaaatat tggaagcttt tgacctctta cgagagtatt
     2941 aagtccatct atagcagata tcccggtttg tatgaactct gaagggtaat cccttgcagc
     3001 aggattcata ggaacaccat taatatcaag tctcatatct ggaattatat taggtccgtc
     3061 atctataggt tcacccatcc cgctgaatac tctgcccaac atatcaacgg aaaccggcaa
     3121 ctcaaggcct ttccctaaaa accttacctt actttctgca acatttattc ctgcagggct
     3181 ttcaaataac tgtactagag ctttatcctt atttacttca agaactttgc aacggcgcac
     3241 ttctccactt gataattcaa tttctcccag ttcgccatat ttcacacctt caacgctttt
     3301 aacaagcatt aatggccccg ccacttctga tatggtttta tattctttaa gcaccaaagc
     3361 tcacctcctt tgatattaac ccatctatct gccggttaag ctcagtctca atatcaaaga
     3421 acacagcatt aacttcatct tcatgagtat acttagccct tccaattctt tctcttacag
     3481 gaatggcaaa cagatcttta atagctactc ctgattccag tgcttttttg cctttatagt
     3541 agaaggtcat tataagttta agcattccaa actgcttatt taacgatgta taagtatcaa
     3601 tctcatggaa tgcattctga tgcaaataat cttcacgtat agatcttgca gcttcaagag
     3661 taagcctgtc agcagatgaa agtgcatcaa taccaaccag tcttactatt tcctccagct
     3721 ctgattcttc ctgcaatact ctcattacat cagatctgag cacactccac tctctggaaa
     3781 tattctgctc catccattta tccagtctgt caagatacaa tgaataactg ttaagccagt
     3841 taattgcagg gaaatgcctt ctataagcca gacctgaatc cagcccccag aaaactttta
     3901 ctattcttaa tgttgcctgg gtaacaggct cggataagtc tcctcccgga ggtgaaactg
     3961 ctcctattgc agtaagcgct ccttcccttc cattcgttcc aaggcttatt actcttcctg
     4021 atctttcata aaactgtgcc aatctggatc ccagataagc tggatagcct tcttcacctg
     4081 gcatttcttc aagtctcccc gacatttctc tcagagcttc tgcccaccgt gatgttgagt
     4141 ctgccatcaa agctacacta taacccatat ctctgaaata ctcggctata gttataccag
     4201 tgtaaataga agcttctctg gctgcaacag gcatgtcaga tgtatttgca ataagtactg
     4261 tacgtttcat aagggactct cctgttcttg ggtcctttaa ttcaggaaat tccaataaaa
     4321 cgtcagtcat ttcattccca cgttcaccac aaccgatgta aactactatg tcagcatcag
     4381 cccattttgc caactgatgc tgtactacgg ttttaccgct tccaaaaggt ccaggtaccg
     4441 ctgctgttcc gccttttgcc aacgggaaaa gagtatctat tactctctga cctgttatca
     4501 agggcgtatc aggaggcagt ttttccttgt aaggcctgcc ttttcttaca ggccactttt
     4561 gtatcatttt aatatttgca atttcaccat tatctttttt aattttggca acagtgtctt
     4621 caatagagta ttcaccttca tatatttctt ctatgattcc ttctatacca taaggaatca
     4681 taattctatg ctcaacaata acagtttcct gaacaactcc aattatatct ccagcttcaa
     4741 ctcttgcccc ttttccaatt acaggcttaa aatgccactt ctcttttcta tcaagagcgc
     4801 ttacttctat acctcttgta atattactgc cgaccatttc cctgattact tccagaggcc
     4861 tttgtattcc atcgaatatt ttcccaataa gcccggggcc taactcaaca ctcaaaggaa
     4921 atcctgtagt ctctaccggt tctcctggtc caagtcccgc tgtttcttca taaacctgta
     4981 ttgaagcttc ttccccatgc atttctatta tttcacctat taaacgctgt tcacttacgc
     5041 ggacaacatc aaatagattt gcatttctca taccttcagc tataactaaa ggacctgaaa
     5101 ctttaactat cctaccctgg cccatatcca atcacttcct tcgtccataa aatcaaaatt
     5161 ctaatttatc ttaatttatc caaacaatat atctgctcct acagctttct ctaaagactt
     5221 ttttattccc ctcacaccta ttcctaaaga gccttgattg ccgggaattg gtataattgc
     5281 aggaagcttg ctgctcttgt aactatcaat acttgcggat attttttctg caacctgctc
     5341 agttatataa attacagcat aatcctcacc tgctaaacga tgtaatactt ttacagcctc
     5401 ttcaggagat aaaaccggaa atacagaaag acctaatatt ttaaaaccaa gaacactatc
     5461 cttatcacct attactccaa ttttatacat aagtatctct cagcctttct cttataatat
     5521 caccggaaat attgtttatt ttacccacca ttataatccg tacattttta atttcgtttt
     5581 cctttgccac aagatatcca attaaaggtt ctataccaag agtcttatac ctggattttt
     5641 tcacgaaatt aataatataa tcatcagcca gtttttcaag ccttgaaagg ttgcctgtat
     5701 taacataatt actgataccc tggctgcaaa tattgaaata gggactgttt tttatttttt
     5761 caacgaaaac ttccatattc gtgtccatat tttcagtgaa aaaggaatca gcaattgtac
     5821 ctccagggat taaactcctg ctaagcaaat cggctgacat attcaaattc tttattctta
     5881 taaatgtttt tatatttgtt aggtctatta taatttttac caattcctta acatattcat
     5941 ggcctgactt ttcagaaatc tcaactatat gcctaaaata tgccttatcc agtattaaat
     6001 caatctgcct tggatcattg gttctgctaa atgtatctaa gcattcgtct atggcctctt
     6061 tcatatataa tggtaatcta tccaattctc tttcatttat tgctgtttta atatgcattg
     6121 gaggcatagt cccagaaact aaaagattat cgtttgcttc tattgctaaa aattctgatt
     6181 ttaataacac tttaatatta tgataatcat tttttaataa aaatatatta aatagttcat
     6241 catcagaggt aacttctctt aacaaaccgt aaaggcgttt ttcttcttcc cttaaaagat
     6301 tttcatattc tccggcatta cctgctagag ttgatgattc aacatatcct gattcaacaa
     6361 taaccttcag tgcttcatcc ggtgattttg aatcaatcat tctgtcaagt ttgcctttat
     6421 ccagcaattt tttttcaata accctgatcc ttccgattgc ataagcataa gcaaaacgtg
     6481 tatcaaccgg caaatgactt acctcccttt tttaagaaaa ttcccttagc ccgttactaa
     6541 agcaatcatg cgaattttcc tttcgcaatg cattaattaa acaatatcct gataatttcc
     6601 gattcaatgt cattatattt catcctgaaa attgtatcaa aagaattgtt tgtttcaata
     6661 tttccagatt ttaatataaa tccgccgccg atatttctgc attcttcgga gaattttatg
     6721 ttaccttgtt ttttcatatt ttttaatctc ttattgacca aatttataaa atcattacct
     6781 aatctttgct tatcttcttt tgagacaatt acttcttcat taccctcagg aaccatatta
     6841 ataatcatat ctgcaagcat tgatttatat tgctcctgcg gtaataaatt caatttctca
     6901 tgtgccttta aaaatgcttc ggcaattacc tcctgctttg ctttaagctt gatttttcta
     6961 ccttcaagct cagcaactgc gataagcctt ttcttttttt cagaaacttc tttttctgtt
     7021 ttctcaatta tggagcccct ttttcttaga gcttcctctt ttgcagactt tataacactg
     7081 tctgcttctt tttctgcctg ctcaatattt tgcatggctt gcatacgagc atcttccata
     7141 attttttcaa ttaatttttc tgctccatcc attcggaaca cacccttctg ccctttatat
     7201 attaataccg aaaaccatta agattgaacc cagcagtgcc aataccgcat atgtctcaac
     7261 cattgcagca aatgtaacag cttttgccag ttcctgaggt ctcttggcaa taattcctat
     7321 accggcggca gcagtcttgc cctgagcaat tgcagattta taaccaacta tagctatagg
     7381 caatgcagct acaaggaaaa gaactccttg aacagtagtc aattctttta caggaccacc
     7441 tacgatacca attttattta gaataacgaa ggctgttaaa agaccatata taccctgtgt
     7501 tcctggtaaa gcctgcagta ataaggtttg agcaaatttt tcaggttctt ccgttataac
     7561 accagctgca acttcaccaa ccatacctac agctcttgct gaccccaatc ctgcaaaaat
     7621 aactgcgcat gctgctccta ttaaagctag gaaatttccg tttaaaagta cctcgagtag
     7681 tttcataatt aaaccttcct cccatcagta atgttaatat attttgtatt tattttaaag
     7741 ggtttataag gtttaccccc accttcataa aatttaccga agaattcaac atattgcaaa
     7801 cggcttgagt gtacaaaagc acccaatgca ttaatcaata tgttgaatac atgtcctcct
     7861 gtaaaaacaa tcagtaagac aataattcca agtatattga agccaagcag tgttcctata
     7921 gtattaataa ctgaagcaat tacaccggta gcaagcccca gagcaagcaa cctggagtat
     7981 gacaatacat cactcataaa acctactgca ttataaaggc ttagtacgcc tttggtaagc
     8041 ttcttgaata tatttttttc tgcccttccc tgagtgagaa ttaaaagtat aacaccgact
     8101 attgtcatgt attttccgat agtcccaaca tttcctccca ccagcagcaa aaccagaccg
     8161 gtgagaaata tataccacaa acctatatcg aatactgcat ccaatacctt gccttccctt
     8221 ataaggctat atccttttaa tcccattgct gtgtacaact gtattcctcc gaaaataaag
     8281 gaccacatca aaagcctcat tggatcatcc aatggattga accagagtgg tggtattgta
     8341 aacttacctg aagatacctg tgtaacaata tctccaaacc aaccgccaaa caaagcaccc
     8401 catataaagg tggaaatacc accaagaaat agtagtttta atagtttgcc aaccgtgccc
     8461 tgaggtttga atttataaat aacaatacct gtagctattg ccattaatag tccatatgcg
     8521 gcatctccaa ccatcaaacc aaagaaaacg aaaaagaaag gtgccataaa agggttcggg
     8581 tctatttccg aaggctttgg aaggctatag agttcggtga caagttcaaa tggctgaaca
     8641 aatcgattat tttcaaggag tattggaaag tcttcatcct tgttcgattc ctctatacta
     8701 acaaagcaat cccactccca ttttgaaata taattcctga agctgttaat attatgttcc
     8761 ggaacccatc cttcaaggat aaaagtgcat tctgtcttta gtagtcttcc taatgcaata
     8821 tttctgtctc tttgtatcgc aagatagtcg taaagtactt ctaaatcttc tcttttgcct
     8881 gcaagggcag ctatttcacc tcttagtgtt tcccttttcc tatcaacttc ttctatgctt
     8941 tttaatgaat tttcaatatt ctgatttata gtacctttca tatcagtaaa acttagtctg
     9001 ttaaaacctt tattttttaa taccctggtt aattcttcct ctctggattt gtgacaaata
     9061 gcctgaatgt acgactgatc cttatccaca tttactaatt gtaaataact ttcaggtata
     9121 tttacactca attctttaaa tatttccttt acatctgctg ctataggaag tacacccatt
     9181 ataacactgg tagtttttgt agatataagc tcgacagggg tattcattga tttccatggt
     9241 gttagagagg caataaggtt ctccagccta ttctcttcca ttcttagttg tgcaagctcc
     9301 tcgtcaagtt tgcttatttt ttcaacggca acccacatat catccttatt ttttaatata
     9361 tcactgaaac gtaatgcatc gacagatctt ttaggagcaa aaagaggctt tttctgctta
     9421 tcatattgat taagatattc caaagcagac cttacctttt ccatatccag ctcaagcgtc
     9481 aatgcttcat cttcattatt gtctttatca acaagacctg tccactcttc agtcagagcc
     9541 gtgtctccaa tatcatttat atccaggaca ccgatattca taagactttc aatgatcccg
     9601 gatttttctt tttcaagacc aatcaaggta atcttatgca ttttaactat tgacatggct
     9661 atttacaatc ctttctacaa ctatatctac agccttgtta atatttgtgc gggccttgtt
     9721 tttaataagc ttcccttctt cttcagcttt tctatatatt ccctctattt ctccctgtac
     9781 ttctttttcc atattcataa gtaaatttgc tgattcctgc tcagcttctt ccactatctt
     9841 tgcaagtacc tcattttttt gcttttctgc atctgacata atgttatgtg ctttagaaat
     9901 agattccttt attaaattat ctgcctcttg ttcggcattt tttatagatc ttactatttc
     9961 gatcgtcact aaatcaccac ctgcatatac taaacatgat aaattcatct ataaatattt
    10021 ccatacattt attaatcata catttgtttt taatattatg caataggcat attgcatatt
    10081 ttaatttcta ttgttgggtg cattttattt atatgtagaa attattggta aggtacataa
    10141 gtggaggatg ctgtagctag gcaggggaaa ctcttctctc aagtaacagg
//
LOCUS       DAOSDD010000162        10153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2510_length_10153_cov_5.177362, whole genome shotgun sequence.
ACCESSION   DAOSDD010000162 DAOSDD010000000
VERSION     DAOSDD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_10153_cov_5.177362"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PK733_13405"
     CDS             complement(<1..923)
                     /locus_tag="PK733_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ11572.1"
                     /translation="MSIIGYFKDVKVRYEADIVVVGSGMAGISAAISAALQGCSVLLI
                     ERFGMLGGNSTTGGVASFCGNTIGQGKVFDMVVEGLEAFGAITPLNPNKDSRVFNHEI
                     LAVVLQELVLKHNVKVLLHTRFVDVICKDGLITECLVCGKSGLEAIRAKQFIDCTGEG
                     ELAARAGFSTMKGGESGYQLPMSLMYFVKYIEENEKRCEIPEGWFEKIDTSDKLPMTS
                     IWPNGPGSNAVKIKIPMFDSTDTESMTAAEIQGRRKMMSVMDYHQRVEKKNWLFDHCS
                     PMIGIREGCRIVGDYILTLEDLRAGRSFDDS"
     gene            complement(970..1728)
                     /locus_tag="PK733_13410"
     CDS             complement(970..1728)
                     /locus_tag="PK733_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ11573.1"
                     /translation="MGNFREVEYSDGVHKLYGYYQDNKSNNNLVLIPGSYTHHSIWEP
                     VIETSNINANIMLVELPGFGKSTPRIPDGTIEEFASLILKLIDAAKIKRFFVGGHSIG
                     GMIAIEMIDHASKRIDGVISCEGWTHASVEKNAFNNLKNELLTLEQMEKRKYFGTIAR
                     TSWTDDEIKAYSKIWRKWGKGKSILDKCEIPVLEIWGDRGLKHKPGREELQIPDKDNI
                     NLVWIENGGHSMLIQYPSLIGKEIASFINNSSKK"
     gene            complement(1889..3502)
                     /locus_tag="PK733_13415"
     CDS             complement(1889..3502)
                     /locus_tag="PK733_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11574.1"
                     /translation="MRKLRSLCIAALVLSIIVAGCVSKSNTQPETAANKTATGESQTT
                     SKDATGEPKSKRLVDKDVEITVMLGESNLVPISQDMLHFKWMYEQTGIKIVFMPVPVA
                     DYNQKKSTLLSTNDLPDIIQVGQSDLNNFASTGIFVDLSKYKDQLLNFYDKVERYPEA
                     KFTFIDGKPFGFPTLNRWDMTRASGLVVRKDIMTKLNIDPPKTFDEYYDMLKKFKAQY
                     PDSVPYVNRNGSKNLISTLGYSLGSGTSIQYDPQAKAYLFMPGKPETKQVLAYLNNMY
                     KEKLLDPDYISVTATQWEEKIANGTGLSYLDNVGFCLKHLTALQKIVGDAEWDLLFMP
                     VNSFGYARGLYISPHQLGRIWAVSSESKKIPEIMKMFNWFYTEEACDMMNLGKEGEDF
                     IRKPDGELEVTEQNRKKYSDEKGLFVQALMHKERGNAAYESFIPYSDLHSYFLATSEF
                     QLNWYKEIQQKDKAYTFPLPTPPFNTDEREKVTSIQAQLNTIAVEMFDKLIMGVEPID
                     AFDKYVQKFKEKGMDELEKIYSDAYNRIK"
     gene            complement(3644..4519)
                     /locus_tag="PK733_13420"
     CDS             complement(3644..4519)
                     /locus_tag="PK733_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11575.1"
                     /translation="MTVKESKGEQLFNVFNVVLLMLLGIITLYPLYYVLIVSVSQSAA
                     VSRGEVAIIPIGLNLNSYKLIIKDPNFLRSFFNTTKYTFVGTLVNLFMSAICAYPLSI
                     RTFSGRKVFTAIIVFTMFFSGGLVPTYLVVKSLGMIDTIWAIALPTAISTWNMIIIRT
                     SFQNIPDSLRESCYIDGANDIVIFLKIILPLSRPVLAVMTLFYATGHWNSFFPELIYL
                     SKKSMFPLQLILRNMLISNQFSELYGDILQTSNVLPTTLNYAAIIVSTIPILLVYPFV
                     QRYFVKGVMIGSLKG"
     gene            complement(4577..5560)
                     /locus_tag="PK733_13425"
     CDS             complement(4577..5560)
                     /locus_tag="PK733_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11576.1"
                     /translation="MLIANAVKKPKQTGNAFKVNIISFIGRIRRAFMRDKYLYLMFLL
                     PLLYYIIFHYVPMYGILIAFKKIKVFRGLGSILEAPWNGAQYFKEYLKDPYFWKLVRN
                     TILIRIYDIAWGFPVPIIFALLLNEVRNNVFKRTIQSITYLPHFLSTVVICGMITTFL
                     ATDGLINNIIKAFGGEAIQFLAKSEWFRTVFIASGIWQNFGWESIIYIASLTAIDVEL
                     YDAARIDGAGRWKQTIHVSLPGIMPTIIILFILNLGKIMNVGYQKILLLYNGATYETA
                     DVISTFVYRRGLIETNFSYATAVGLFSTVIGLMFIISANKISKKVGETSLW"
     gene            5909..8218
                     /locus_tag="PK733_13430"
     CDS             5909..8218
                     /locus_tag="PK733_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11577.1"
                     /translation="MIRKKFKFYNKLTFYFSLVLLICIVSMATVLYFFSTNAINEEVF
                     NTNVNNLNYIVKNMEITIRSIDFITSQIALDPNVTYFMRNRFSDDYMRLINLISNMDG
                     HKVINPLIHSIYLYYKGNNAIISSPDGKVEISSFSDIDWIQELTYKNQTQWILNRKIK
                     ITSTGKGNFDILTYVRYLPIGSIGPEGALLININVNELYKLYQSSNDNSSGIVVFNEY
                     NNKIFTTINEDMDFNPILKNITSTEKHSGYDQIKYKGRTYAMAYSTSSNGWKYIMTTP
                     ISKLMSMQNIIRNIIIACTMCMLIIGLQLSLVFSRRLYNPINNLMNSFQYGADKPGIV
                     DEFGIIERSVNSLIHEKGLLEEKNTKSLNYAVERVVIGVLNGNVMLKALEDFNGDIGI
                     QLHKCNSYMVIVAELDDYKNLSGEYCPYEHIYHIQSLILKKFEDFKIIKDVNFCKGVS
                     TYPYQGACLLALNVIMSDESILLKQVFSICNNIRDEINTQYKANISIGFSIIGKSSSE
                     IYGLYHQACNAVKNGIFQGKNCVSYYGQISFWDNSVFIFPIKQEEAILKSLLSLDRQN
                     VSVSIRDFVEYIKNSKGLEHNQVFYIFAHLLGSITKAAYELGGSVLEVWEGKDMYKSL
                     SQCNYLEDIEELMVSLANHYINYFEEKKSNKYKTIVESAIEYMMSNYANHQISLNLIS
                     EKVYLSSSHFEKIFKDITGKSAIDYLNNIRMEKAKALLKDNNLKVEVIAEKVGYTTSR
                     GFIRAFKKYENITPGQYRQNLASNLTDRI"
     gene            8302..9177
                     /locus_tag="PK733_13435"
     CDS             8302..9177
                     /locus_tag="PK733_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ11578.1"
                     /translation="MSTSTSIYFSRFNSTCIPIIDSIKRCAAIGYKVMDIDFHSGIKY
                     DTYELKSSNWENWIETVGRIANEYSIIFSQAHAPFYNVADPEFIHREYYDEMVRRSII
                     ASGKLGIKWMAMHAGTALDENRFVEISKKRNMKYFDPYIDLAAKNNVGIAIENMQEYL
                     EEWRTKPRRRYTAGIEELCDLVDSFKTNSVGIVWDFGHANITGYDQVKSLKYLGKRLK
                     ATHVHDNKGLFDDHTLPFLGTINWYPIMQTLKEIGYEGDFTYELARYTNNIPEELIDD
                     AIRLSISIGNYLLTL"
     gene            complement(9336..>10153)
                     /locus_tag="PK733_13440"
     CDS             complement(9336..>10153)
                     /locus_tag="PK733_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11579.1"
                     /translation="HPSMKEALAYIRKLYSEELVLQDWMAIKGAQADAYIDSGKLGLF
                     YGFWTYGANREISVKTLFPEAEIIPIALPVGPNGLSGNRSSGYVNSRTFSLSAKIEYP
                     ERALEFLDYFHGSDGMMFGRYGIEGIHWKWKSGKPTPKNLDELNKRVDDIEFTEQYYT
                     DQKAVIIESLNLYQPQQDITLLPGWSEDHVTAQKFTRTQPVIFDDSDGILSDAKAKYA
                     AALNTLRDETFTKIIMGEYDLDKFDEYVAKWKAQGGDELIKDLTEKYKNLLGK"
BASE COUNT         3384 a         1832 c         1578 g         3359 t
ORIGIN      
        1 atagaatcat caaaacttct acctgccctt aaatcttcta aagtaagtat ataatcacca
       61 actatccggc aaccttccct aataccaatc attggggaac aatggtcaaa taaccagttc
      121 ttcttctcaa ctctctgatg ataatccatt actgacatca ttttacgcct gccttgtatc
      181 tccgcggctg tcattgattc cgtatcagtt gaatcaaaca tcggaatttt tatctttaca
      241 gcatttgacc cgggtccgtt aggccatata cttgtcatag gcaatttatc agaggtatca
      301 atcttttcaa accatccttc aggtatttca caacgttttt cattttcttc aatgtatttg
      361 acaaagtaca taagtgacat aggaagctgg tacccgcttt cgccaccttt catagttgaa
      421 aatccggcac gcgcagccaa ttcgccttca cctgtacagt ctataaattg cttagcccta
      481 atagcttcca gtcctgattt accacagaca aggcattcgg ttattaaccc gtctttgcaa
      541 attacatcaa caaaacgggt atgtaaaagc actttaacat tgtgctttaa aacaagttcc
      601 tgaaggacaa ctgcaagaat ttcatggtta aaaactcttg aatctttatt gggatttaac
      661 ggggtaatag caccaaaagc ctccagccct tctactacca tgtcaaatac ctttccctgc
      721 ccaatagtat taccacaaaa agatgcaact cctcctgttg tactatttcc tcccagcata
      781 ccaaaacgtt caatcagcaa gacactgcaa ccttgtaatg cagcacttat agcggcagaa
      841 atacccgcca taccagaccc tacaacaaca atatcagctt catagcgaac tttgacatcc
      901 ttaaaataac caattatact catatttatc actccatcaa taaaaatatg cctatataaa
      961 tatacatact tatttcttac tggaattatt aataaatgat gctatttcct ttccgattaa
     1021 gctagggtat tgtataagca ttgaatggcc accattttca atccacacaa gatttatgtt
     1081 gtctttatct ggtatttgca gctcttctct acctggctta tgctttaatc ccctatctcc
     1141 ccatatctcc aataccggta tttcacattt atctaatata gattttcctt ttccccactt
     1201 tctccatatt tttgaatatg cttttatttc atcatcagtc cagcttgtgc gggcaatagt
     1261 accaaaatat tttcttttct ccatctgctc cagagtcaat aattcattct ttaaattatt
     1321 aaaagcattt ttctccactg aggcatgagt ccatccttca cacgaaatta caccatctat
     1381 ccttttagaa gcgtgatcta tcatttcaat agcaatcata cctccaatac tgtgcccgcc
     1441 tacgaaaaat cttttaattt ttgcagcatc aataagttta agaattaatg atgcaaattc
     1501 ctcaatagta ccatctggta tacggggtgt gctcttccca aaccccggca actctacaag
     1561 cattatgttt gcatttatat ttgatgtttc tataacaggc tcccatattg aatgatgtgt
     1621 atagctcccg ggaataagga caagattatt attacttttg ttgtcttgat aatatccata
     1681 aagcttatgt accccatctg aatattcaac ttctctaaaa ttacccattt taactgcctc
     1741 ctttaaagta taaaagtata gggacactca ttgttaaatt ctcgcaacat attctcaatg
     1801 ctatcaaaaa tcattctaga agacatcact tttctaattg gcatagtagc attaagtgtc
     1861 cctatgttcc caacatacaa taaataattt atttaatcct attataagcg tcactgtata
     1921 ttttctccag ttcatccatt cctttttcct tgaatttttg aacatattta tcaaaagcat
     1981 caattggttc aacgcccatt attagtttat caaacatttc cacagcaata gtatttaatt
     2041 gtgcttgaat acttgttact ttttcacgtt catcagtatt aaatggcgga gttggcaaag
     2101 gaaaagtgta cgctttatct ttttgctgaa tttctttata ccaatttaat tgaaattcgg
     2161 aagttgccag gaagtaggag tgtaagtcag aatatggtat aaatgattcg tatgcagcat
     2221 ttccacgttc tttgtgcata agagcctgta caaataatcc cttttcatca ctgtattttt
     2281 ttctattttg ttcagtaact tctaattctc cgtcaggctt gcggataaaa tcttcacctt
     2341 ctttacccag gttcatcata tcacaagcct cttctgtgta aaaccaatta aacatcttca
     2401 taatttcagg aatttttttg ctttcagaac taactgccca tattctacct aattgatgcg
     2461 gtgaaatata taatcctctg gcatatccaa aagaattaac cggcatgaag agtaaatccc
     2521 attctgcatc accaacaatt ttttgtaatg ctgtaagatg ttttaagcaa aaacctacat
     2581 tatcaagata ggataatcct gtcccatttg ctattttttc ttcccattgt gtagccgtaa
     2641 cagatatata atcaggatca agcagcttct ccttatacat attattaagg taagccagta
     2701 cttgctttgt ttcaggttta cctggcataa ataaatatgc ctttgcttga ggatcatact
     2761 ggatcgaagt accgctacca agagaatagc ccaacgtgga tataagattt ttagaaccgt
     2821 ttcggtttac atatggtaca ctatccggat attgtgcctt gaatttcttc agcatatcat
     2881 aatactcatc aaaagttttt ggcgggtcaa tattaagctt tgtcataata tctttacgta
     2941 caaccaatcc acttgccctg gtcatatccc atctattaag tgtaggaaac ccaaaaggct
     3001 tgccatcaat aaaagtaaac tttgcttcgg gatatctctc tactttatca tagaagttca
     3061 gaagctggtc tttatactta cttaaatcaa caaatatgcc tgtagaagca aaattattca
     3121 ggtcagactg tcctacctgt attatatcag gtaaatcatt tgtagaaaga agggtactct
     3181 ttttctgatt gtaatcagca acaggtacag gcataaatac tattttaata cctgtttgct
     3241 catacatcca tttgaaatgc agcatatcct gtgatattgg cacaagatta ctttcgccta
     3301 acataacagt aatttctaca tctttatcca ccaatctttt gctttttggt tcaccagttg
     3361 catcttttga tgttgtttgg ctttccccag tagctgtctt gttagcagca gtttcgggtt
     3421 gggtatttga ttttgataca catcctgcta cgataataga taatactaaa gcagcaatac
     3481 acagtgacct caattttctc attacaattc ctccttcatt tcaatttgat taatatttta
     3541 attggcattg taatacagtt tatttaaaag ccagcttatc caggcttaaa ctcataatat
     3601 ctctatagta tatattttct tatttatatt ttcccatgac gggctatcct tttaaagacc
     3661 ctatcattac tccttttaca aagtatcttt gtacaaatgg atatactaac aatattggaa
     3721 tcgtagaaac aattattgct gcataattca aagtggtagg aagaacatta gatgtttgca
     3781 gtatgtctcc atagagttcc gaaaactgat ttgatattaa catatttcta agtattaatt
     3841 gtaatggaaa catactcttc ttactcaaat aaattaattc cggaaaaaag ctgttccagt
     3901 ggcctgtagc atagaacaaa gtcataacag cgagaacagg ccttgaaagc ggcaatatta
     3961 ttttaagaaa tattactata tcatttgctc catcaatgta gcaggattct ctcaatgaat
     4021 caggaatatt ctggaatgat gtcctgatta ttatcatatt ccatgtagat atagctgtag
     4081 gaagagctat agcccatata gtatctatca tccccaggct tttaacaacc agataagtag
     4141 gtaccagacc accggaaaaa aacattgtaa atacaataat tgcagtaaaa acctttcttc
     4201 cggagaatgt tctaattgaa agaggatatg cgcatattgc agacataaac aggtttacta
     4261 aagtacccac aaaagtatat tttgtagtgt tgaaaaagga ccttaaaaaa ttaggatctt
     4321 taataataag cttgtatgag tttaagttca agcctattgg gattattgct acttcgcccc
     4381 ttgaaacagc cgcgctctga cttacagata caataagtac ataatataat ggataaagag
     4441 taataattcc taataacatt aataatacaa cattaaaaac attaaataat tgttctccct
     4501 tactctcttt tactgtcata agcaacaatt ccccccataa caaacaatta acaacaaaga
     4561 gattattaaa aacagcttac caaagactgg tttcgccaac ttttttactt attttgtttg
     4621 cactaataat aaacattaat cctattactg tagaaaacaa ccctacagct gtagcataac
     4681 tgaaattggt ttctataagc cctcttctat atacaaatgt tgaaattaca tcagcggttt
     4741 cataagttgc gccattataa agcaaaagta ttttttgata tccaacattc ataattttac
     4801 caagatttaa aataaatagt attattatag taggcataat tccgggtagt gaaacatgaa
     4861 tagtttgttt ccaccttcca gccccatcta ttcttgctgc atcgtataat tcgacatcta
     4921 ttgcagtcaa tgaagctata tatatgatac tttcccagcc aaagttttgc cagattcctg
     4981 atgctatgaa aacagtcctg aaccattcag actttgcaag gaactggata gcctcgcctc
     5041 caaaagcttt gattatgttg tttatcaatc catctgtagc aaggaatgta gtaatcattc
     5101 cgcatattac tacagtagaa agaaagtgag gcaggtatgt aatagattgg atagttcttt
     5161 taaaaacatt atttcttact tcatttaaaa gaagtgcaaa tatgatgggt acaggaaatc
     5221 cccatgctat atcataaatt ctaattaaaa tggtattccg taccagtttc caaaagtacg
     5281 ggtctttcag gtattcttta aagtattgag caccattcca gggggcttcc agtatacttc
     5341 ccagtcccct gaataccttt atttttttaa aagcaattag aataccatac ataggaacat
     5401 aatggaatat tatatagtac agaagaggca ggagaaacat aagataaaga tatttatctc
     5461 tcataaaagc tcgtctgatc cttccaataa aacttataat atttacttta aaagcatttc
     5521 cagtttgttt tggttttttg actgcatttg caatgagcat atatatcctc ctgttaatta
     5581 tgtaaaaaat actcaaacga ttcttgttta tagttttttc tatataacca caacatatgt
     5641 accattttct tacgatactt gaaatacaaa atatattcat aaagcatatt gctatatata
     5701 taattttaca tattattgcg cataggtaaa gaaccatttt taaaaaataa ggcccatttt
     5761 tctactacgg cactaataaa cataccattt agcatttgac agtgacattt agcatctaaa
     5821 agcttgatat gcctgtaaat atgtgcttta atataggtac ttattcattt gctggatgat
     5881 ttacgaatcg ttggtggagg caaaaaaaat gattagaaaa aagtttaagt tctataataa
     5941 gttaaccttt tacttctcgt tagtattgct tatctgtata gtaagtatgg caacagtttt
     6001 atactttttc tcaaccaatg caataaatga agaagtgttt aatacaaatg tgaataacct
     6061 taattatatt gtaaaaaata tggaaattac tataagaagt atagatttta tcacatcgca
     6121 aatagctttg gatccaaatg ttacatattt tatgcgtaat aggttttctg atgattatat
     6181 gaggttaata aatttaatat caaatatgga tggccataaa gtgataaacc ctctcattca
     6241 ttctatctat ttatactata aagggaataa tgctataata tcttctcccg atgggaaggt
     6301 tgaaatatca tcttttagtg acatagattg gatacaggaa ctaacttaca aaaatcaaac
     6361 acaatggatt ttgaaccgta aaataaaaat tacaagtaca gggaagggaa atttcgacat
     6421 tttaacatat gttcgatatt taccaatagg ctctatcgga cctgaaggtg ctttattaat
     6481 taacataaat gtaaatgaac tatataagtt gtaccaaagc agtaatgata attccagcgg
     6541 tattgttgtg tttaatgaat ataataataa gatttttact actattaacg aagatatgga
     6601 ttttaacccc atattaaaaa atataacttc cacagaaaag cattccggtt atgatcagat
     6661 taaatataaa ggtagaactt atgcaatggc atatagcacc agttcaaatg gctggaaata
     6721 cataatgact acaccaatct ccaagcttat gtctatgcag aatattatca gaaatataat
     6781 cattgcatgt acaatgtgta tgcttataat tggtctgcag ctttctctgg ttttttcaag
     6841 gcggctatat aatcccataa acaaccttat gaattcattc caatatggtg ctgataagcc
     6901 cggtattgtc gatgagttcg gaattataga aagatctgtc aattccttaa ttcatgaaaa
     6961 ggggttgctt gaagaaaaaa acacgaaatc attgaattat gcagttgaaa gagtagtcat
     7021 cggagtgctc aacggaaatg ttatgttaaa agccttagaa gattttaatg gcgatattgg
     7081 tatacagctt cataaatgca atagttatat ggtaattgta gcagaattag atgattataa
     7141 gaatttgtcc ggtgaatatt gtccatatga acatatatat cacatacaaa gtttaatttt
     7201 aaaaaaattt gaggatttta aaatcatcaa agatgtgaat ttctgcaagg gtgtatcgac
     7261 atatccttac caaggcgctt gcttacttgc ccttaatgtt attatgagtg atgaaagcat
     7321 tcttttaaaa caagtttttt ctatttgcaa taatatccgt gatgaaataa atacccaata
     7381 taaagcaaat atttctattg gttttagtat tataggaaag tcttcatcag aaatatatgg
     7441 cttatatcat caagcttgta atgcagtaaa aaacgggata ttccaaggga aaaattgtgt
     7501 aagttattac ggacagataa gtttctggga taattcagtt ttcatatttc caataaagca
     7561 ggaagaggca atccttaaat cacttctctc tcttgataga caaaatgtat ctgtatcaat
     7621 ccgtgatttc gtagaataca ttaaaaactc caaagggtta gagcataatc aagttttcta
     7681 tatttttgcc catcttcttg gttctataac taaagctgct tatgaattag gtggaagtgt
     7741 gcttgaagtt tgggaaggta aagatatgta taagagcctt tctcaatgta actatttaga
     7801 agatattgag gagctgatgg tttctttagc aaatcactat ataaattatt ttgaagagaa
     7861 aaagagtaat aagtataaaa caattgtgga gtcagctatt gaatatatga tgagtaatta
     7921 cgcaaaccac caaatatcgc taaaccttat atctgaaaag gtatatttga gctctagcca
     7981 ttttgaaaaa atctttaaag acattacagg taaatcggca attgattatt taaacaacat
     8041 ccgcatggaa aaagccaaag ccctacttaa agataacaat ttgaaggtag aagttattgc
     8101 cgaaaaagta ggatatacaa catcaagggg tttcatacgg gcattcaaga aatatgaaaa
     8161 tatcacaccc ggacaatata gacaaaacct cgcgtctaat ttaacagata ggatatgatt
     8221 attcacacgc ttatactggg taaatagtct gccataaaga taagttacaa aaagtaataa
     8281 aaggagatgg ttaaaattag attgtcaact tcaacaagca tatatttttc aagatttaac
     8341 agtacttgta taccaattat tgattctatc aaacggtgtg cagcaattgg ctataaagta
     8401 atggacattg attttcatag cggcatcaag tatgatactt atgaattaaa aagttcaaat
     8461 tgggaaaatt ggattgaaac ggttggaaga atagcaaatg aatatagtat tattttttca
     8521 caagctcatg ccccatttta taatgtagca gatccggaat ttattcaccg tgagtattac
     8581 gatgaaatgg taagaagatc aatcattgca tcaggaaaat taggtataaa atggatggca
     8641 atgcatgccg gaacagcatt agatgaaaac agatttgtag aaatatctaa aaaaaggaat
     8701 atgaagtatt ttgatccata tatagatctc gcggctaaaa ataatgttgg catagctata
     8761 gaaaatatgc aggaatatct tgaagaatgg agaacaaaac cccgtcgcag gtatactgca
     8821 ggtattgaag aattgtgcga ccttgttgat tcatttaaaa caaatagtgt aggtatagtt
     8881 tgggatttcg ggcatgctaa cattacagga tatgatcagg ttaaaagcct gaagtattta
     8941 ggaaagcgct taaaagctac acatgtccat gataataaag gtttgtttga tgatcataca
     9001 ttgccttttt tagggacaat aaattggtat ccgattatgc aaacacttaa agaaattggg
     9061 tatgaaggtg attttacata tgaacttgca agatatacaa ataatatacc agaagagctt
     9121 attgatgacg caattaggtt atcaataagt atagggaact accttttaac tctttaagca
     9181 tctaacaata ttttcgtcta tcctctcact ctaatcaata aggtattccg gggaaaaact
     9241 atgtccagca ttagcgaaag ttaaatatat caaaataaga gacactccga agaataatcg
     9301 gggtgcctct aatagttgga agttagcaag ttgctttatt tacccaacaa gttcttatat
     9361 ttttcagtca gatcttttat aagttcatcc ccaccctggg ctttccactt agcaacatat
     9421 tcatcaaatt tatcaaggtc atattcaccc ataataattt ttgtaaaagt ttcatctctt
     9481 aaagtgttca aggctgctgc atatttagcc tttgcatcac ttagtatacc atcagaatca
     9541 tcaaaaataa ccggttgagt tctggtgaat ttctgtgccg taacatggtc ttcggaccat
     9601 ccgggaagta gagttatatc ctgctggggc tggtaaagat tcaatgattc aatgataact
     9661 gctttctgat ctgtgtaata ctgttcagta aactcaatat catctacccg tttgttcagt
     9721 tcatccagat ttttgggggt aggttttccg cttttccatt tccaatggat accttcaata
     9781 ccataacgtc caaacatcat tccatcactt ccatgaaagt aatcaagaaa ttctaaagct
     9841 ctctccggat attcaatttt agcagataag gaaaatgtcc gtgaatttac atatcctgat
     9901 gacctatttc ctgatagtcc attaggacct acaggcagag ctatcggtat aatttctgct
     9961 tcagggaata atgtttttac gcttatttcc ctatttgctc catatgtcca aaaaccataa
    10021 aataggccta gtttaccgct atctatatat gcatctgctt gtgcaccttt aatggccatc
    10081 caatcctgta aaactaattc ttcactatag agtttcctaa tgtatgcaag agcttctttc
    10141 attgaagggt gta
//
LOCUS       DAOSDD010000163        10131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2517_length_10131_cov_5.752977, whole genome shotgun sequence.
ACCESSION   DAOSDD010000163 DAOSDD010000000
VERSION     DAOSDD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2517_length_10131_cov_5.752977"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK733_13445"
     CDS             <1..219
                     /locus_tag="PK733_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOJ11580.1"
                     /translation="KNAHIESFHRIFEDECLGRWEFESYEEAYKAVMDFMERYNYRRI
                     HRSIFNLSPAEFHKATLNGTARTLTITL"
     gene            complement(431..1876)
                     /locus_tag="PK733_13450"
     CDS             complement(431..1876)
                     /locus_tag="PK733_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11581.1"
                     /translation="MMYKVFIADDEKAIIRGLKSSINWNELGFQIAGEADNGIDAYEK
                     ILEIKPDVVFTDIRMPGMNGLELIKKVSEFSQDILFVVISGYAEFAYAQKAVKYGVLG
                     YCLKPFDEDEIAGILNKARSVLGKQISSMKMELISLIEDGSIESQQRIEKIMNAAGLE
                     LSDSKGLLAVVVIGREEPVIFNDAAYINLKIGKNTYLYLVQDFSLRNIADYMSDDMLE
                     CVKGVGVSKTYYSVESISDAIDEAFVAANQYFMTGKKGIYFYQKYDKDELRNAFFQLE
                     DAIIKRDLNMVRNSLDNAEAVLSKGVYNIKHAFQIYNIFMHTFYTMHPEKYDNYIYDY
                     EKLTSYFGGIHDMFEYLNGLIDDYAGINIEYQFTEVKSEIFKSILLYINENFCKDICL
                     QAIAEKFCINSSYISQLFKKHTGETFTEYIGNLRLNYACNLLKTTALNVNEVAEKVGF
                     NDYYYFTKVFKKMIADRPPLFGEFDLKLSFS"
     gene            complement(1866..3665)
                     /locus_tag="PK733_13455"
     CDS             complement(1866..3665)
                     /locus_tag="PK733_13455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11582.1"
                     /translation="MLYFLKKLSIGTQLLLLAISTGIIMLIIILSIYFDVSKVVAKYN
                     NEYTDNIMFQVKKNIVGNCDFMDRLLTSIAYNRVVQNYTIEKDDEERFSMYDDINNFF
                     ITLSEMNEGIIDFVLLDENGEKVYFLKGENDEVKETIRNLPIKNDHYYTGVKRFYIKD
                     EFRDCFIIASNMYSILSGNITGSRIGTAAIVFDAKALSLDFDKSLHESATSFYLLDRN
                     NKIYSTSQLSSLDKNTEQIAGYAGYKSGRYVTKADGKRYVINKEDLREMHGKIISVVP
                     EEKLFYDLEWARTLTIAFFVIALFLLSIPFAVIINNIVSPLKKFMKFITSLKSGNLKG
                     LKQRINLEGYEEIVIMAGEFNGLLDEIDDLTHRLVTTTTRLYATELEKKKSELEYLKS
                     QINPHFLYNTLEVMRSSATDEGAYTTAAMVKALGKIFRYSVKGEYIVRLYEEIEITKY
                     YLQIQQARFDGRFKFIYQMTDESLNCSIPKMILQPIVENAIFHGLETKLGEGHLWIES
                     KIDEYQNLYISIKDDGTGMDREKLNYFQKILNEGQSKIVSSEGSGMGIGIINVNNRIK
                     LIYGSNYGINIESILGHGTEVIIKIPTRRGNDV"
     gene            complement(4251..5891)
                     /locus_tag="PK733_13460"
     CDS             complement(4251..5891)
                     /locus_tag="PK733_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11583.1"
                     /translation="MKKVKRVIVILLVVGMIFGLLAGCEKKNGKDEPTGTNAQTSNKT
                     EASNPLSEKIEISFALWGIGQAITEAPDAVREKIYNELNISIKPMNVTWDDYTQKIQI
                     WAASSQLPDIFAIDVIGTANYKNWIEQGIVRPLPSDLSPYPQINKIMNSPGFDIYKYP
                     IGDPNGKIYAIPRLNHLDIDDWSCDTGIHLRKDWMQNVGITKEPENMDEFIALMKAFV
                     ENDPDKNNKKDTIGLTCYNSDWLTWFFLGYEPGIQSGGWVRDKENPGKWIPAFMTKDC
                     LTGMKELKRLYDAGGLDRDLATLKGEEGRDKFAAGKAGAYAHDVTPSTLGYVQTQFEK
                     VNENIKYKDIVTILKPFKHSDGNYYRHISNPAWSETYINAKCDDKKTDRVMRLFDYVL
                     DEEGYNLIHFGIEGKEWHKEDGKIVLTPQKDGEGKDIPLSVSYPFVKCGFIAEWSGTR
                     QWSAPTVNPELQKMSSDLNDWLQANAKPVPTDLRIPLLDVPSKDKATARTGEIIIKCL
                     LSDNVEKTWNEIVSSYRANGYDKLIEEINEACAKANIK"
     gene            complement(6114..7004)
                     /locus_tag="PK733_13465"
     CDS             complement(6114..7004)
                     /locus_tag="PK733_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11584.1"
                     /translation="MDYSSYRIKKDGFDTFNYIIMIILAVITIFPFYYVVIISFADYA
                     AVQKHLIYIFPTSINLDAYKLVFKGEMFLSSFFVSIFITVTGTVLSMIFSTASAYALS
                     KSFVPGNKMMFNFILVPMFFSGGLIPYYLTIQKLGLIDKIWVLIIPGAINSFYLILLK
                     NFFKELPASIEESAKIDGANDLYILYRIVLPMSAPVIATISLFYAVDRWNDYYTALLY
                     ISNRKLFPLQLVLREAILDFAQIMASPIGAEIARNNRPTYTKALQMAIITVATVPILC
                     VYPFIQKHFTKGILFGAVKE"
     gene            complement(7017..8057)
                     /locus_tag="PK733_13470"
     CDS             complement(7017..8057)
                     /locus_tag="PK733_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11585.1"
                     /translation="MKLIWNVKPDYLERLIVMEKERINLMSNTKISEKNINIIPIKNK
                     PLWKQILDCKYFYIMFIPTFVLYFIFSYVPLYGIQLAFKDLNFAKGITGSSWTSMHGL
                     KHFYELLSNMEFQKAFSNTIIISIQRIIFQFPVPIILALLLNEIRKDSTKRIIQTVLT
                     FPHFLSWIVVTGIFFNLLGDAGVLNEILAALGLKKVHFLTQASTFRGLLYITNNWKEA
                     GWGTIIYLAAIAGISVELYEAAIVDGAKRFQLMRYITWPAIKSVIGVMLILAIANIMN
                     AGFDQIFNMYNAAVYDKADIIDTYIFRRTFITGLDFASSTAIGLFKSVVNFIMLFAGN
                     LAVKKITGKGIY"
     gene            complement(8501..8950)
                     /locus_tag="PK733_13475"
     CDS             complement(8501..8950)
                     /locus_tag="PK733_13475"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOJ11586.1"
                     /translation="MDSDIGCYNNCAASCTQAVEKILKHLFITFDLPFETRLSESHNL
                     NALLRELVKTFPELKSLTKQCRFLNDFYIEIRYPGDSFEWINYDTAMQCYQYAKEVNE
                     GIDAIIQNPLYEQKLLEAFKYGYKNYKIEKCGGFCSKPNTKGIGELT"
     gene            complement(9009..9389)
                     /locus_tag="PK733_13480"
     CDS             complement(9009..9389)
                     /locus_tag="PK733_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ11587.1"
                     /translation="MLIKSKIDGLDIPFDYKFFIFLLTEKFTENENIEKIYLFGSCAV
                     GKMTDKSDIDFAVIVNDKIPANRNFRLNIIDEIEDLLIEQESVTNIPYDIVFFNRMDF
                     ERNKKISISVVNDIEREGMVLYER"
     gene            complement(9578..9868)
                     /locus_tag="PK733_13485"
     CDS             complement(9578..9868)
                     /locus_tag="PK733_13485"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOJ11588.1"
                     /translation="MSHLKKGAFCMVYVNGFVPYYWSSPGKAELDFVIQNREGNIIPI
                     EVKSADNVRAKSLAAFVSLYKPPYSIRISARNFGFENNIKSVPLYGVFCLER"
BASE COUNT         3082 a         1932 c         1532 g         3585 t
ORIGIN      
        1 aaaaatgctc atatcgaatc atttcatcgt atatttgaag atgaatgcct gggacgttgg
       61 gagtttgaga gttatgaaga agcatataaa gctgttatgg attttatgga acgttacaat
      121 tacagacgta tacatagatc aatattcaat ttaagtcctg cagaatttca caaagcaaca
      181 ctaaatggga ctgcacgtac tcttacaata acactgtaga aagcagcttt tatgccgcga
      241 aagcctattt acaatgagtg ctggcatgtt aggctttgtt gccgggcatc aaccattcta
      301 cagagacatt acttcttgtt cagcatgggg gatggccctg atgcttatag acatgccagc
      361 tgaggctcat tgtaaatagg atcgtggaat aaatgcggaa gtattggtgg aaatgacaaa
      421 acgaggattt ctaagaaaaa ctaagcttta ggtcaaattc tccaaacaaa gggggtctat
      481 ccgctatcat ctttttgaaa acttttgtga agtagtagta atcattaaat ccaacctttt
      541 ctgcaacctc attcacatta agtgctgttg ttttaagaag attacatgca tagttgagcc
      601 ttagattgcc aatatattct gtaaaagttt ctccagtatg ttttttaaaa agctggctaa
      661 tgtagcttga gttaatgcaa aacttctcag ctatagcttg caggcatata tccttacaaa
      721 agttttcatt tatataaaga agtatgcttt tgaatatttc actctttact tcagtaaatt
      781 ggtattcgat attgatcccg gcataatcgt caattaatcc atttaaatat tcaaacatat
      841 catgaattcc gccgaaatag cttgttaact tttcgtaatc ataaatatag ttatcatatt
      901 tttcaggatg cattgtataa aaagtatgca taaaaatgtt gtatatctga aaagcatgtt
      961 ttatgttata tacaccttta gataaaactg cttcagcatt atcaagagag ttcctgacca
     1021 tattcaaatc cctttttata attgcatcct caagttgaaa aaaagcattc ctcaattcat
     1081 ccttatcata tttctgataa aaatatatac cttttttgcc ggtcatgaaa tattgattcg
     1141 cagccacaaa agcttcatca attgcatcac ttatagattc gactgaataa taagttttgc
     1201 ttactccaac acctttaaca cactcaagca tatcgtctga catgtaatcg gctatatttc
     1261 gaagagagaa atcctggacc aaatacaaat atgtgttttt tccaatcttc aagtttatat
     1321 atgcagcatc attgaaaata accggttctt ccctgccaat tactacaaca gctaaaagtc
     1381 ctttactatc tgatagttcc agacccgctg cattcataat cttttctatc ctctgctgag
     1441 attcaatgct gccgtcttct attaagctta taagctccat tttcattgaa gatatttgct
     1501 tgcctaatac acttcttgct ttattcaata tgccggcaat ttcatcttca tcaaaaggtt
     1561 ttaagcaata tcccaacact ccatatttca cggctttctg tgcataagca aattccgcat
     1621 acccgcttat aactacaaaa agtatatcct gggaaaactc agagactttt ttgatcagct
     1681 ccaagccatt cattccaggc atccggatgt ctgtaaaaac cacatccggt ttgatttcaa
     1741 gtatcttttc ataagcatca ataccgttat ccgcttcacc tgcaatctga aatcccagct
     1801 cattccagtt tatacttgat ttcagacctc taatgattgc tttttcatca tctgctataa
     1861 agactttata catcatttcc cctccttgtt ggaattttta ttattacttc agtcccatgc
     1921 ccaagtatac tttctatatt aataccatag ttactgccat aaataagttt tatacgatta
     1981 ttaacattaa taattccaat gcccatgccg ctgccctcac tagaaacaat tttactctgg
     2041 ccttcattta atatcttttg aaaatagttg agcttctctc tgtccatacc cgtaccatca
     2101 tcttttatgc ttatatataa gttctggtat tcatcaattt tgctttcaat ccataaatgc
     2161 ccttctccca gctttgtttc caggccgtgg aatatagcat tttctactat aggctgcagg
     2221 atcatctttg gaatactgca atttaaagat tcatctgtca tttgataaat aaatttgaat
     2281 ctgccgtcaa accttgcctg ttgaatctgc aagtagtatt tggtaatttc aatttcttca
     2341 tacagtctaa caatatattc acctttaaca ctatatctga aaatttttcc aagggctttt
     2401 accatagccg ctgttgtata tgcaccttca tctgtagcac tcgatctcat tacttccaaa
     2461 gtattatata aaaagtgagg attaatctga ctctttaagt attccagttc ggattttttc
     2521 ttttccagtt ctgttgcata aagcctggtt gttgtagtta ccagtctatg ggtaagatca
     2581 tctatctcgt caaggagccc attaaattct ccggccatga ttactatttc ttcgtaacct
     2641 tcaagattga ttctttgttt caatcctttc aaattccctg attttaatga tgtaatgaat
     2701 ttcatgaatt ttttaagggg gcttactata ttgttaatta ttactgcaaa tggtatggag
     2761 agaagaaata atgctattac aaagaatgca atagttagtg tccttgccca ttcaaggtcg
     2821 taaaaaagtt tttcctcagg tacgacactt ataattttgc catgcatttc gcgaagatct
     2881 tccttattta taacatacct ttttccatct gcttttgtta catatcttcc tgacttatag
     2941 cctgcatagc cagcaatttg ttctgtgttt ttatctaatg atgataactg gcttgtagaa
     3001 tatattttat tatttctatc caataagtag aaactcgttg ctgactcatg taaacttttg
     3061 tcaaaatcaa ggcttaatgc tttagcgtca aatactatag ctgcagtccc tattcttgac
     3121 ccagtaatat ttccgctgag tatggaatac atgttagatg ctattataaa gcaatctcta
     3181 aattcatcct taatataaaa tctttttaca ccagtataat aatgatcgtt ttttatgggc
     3241 agatttctga ttgtttcttt aacctcgtca ttttcacctt ttaaaaaata cactttttct
     3301 ccattttcat caagcaatac aaaatcaatt attccttcat tcatctccga aagagtaata
     3361 aaaaagttat taatatcatc atacattgag aatctctctt catcatcctt ttcaatggta
     3421 taattttgaa caacccgatt atatgctatg ctggtaagaa gcctgtccat aaaatcgcaa
     3481 ttgccgacta tatttttttt gacctggaac atgatattgt ctgtgtactc attattgtac
     3541 ttagctacaa ctttggagac atcaaaatat atagaaagaa ttattataag cataataatt
     3601 ccggtggata tagcaagcaa aagtaattgt gtccctatgg acagtttttt tagaaaatat
     3661 agcattaata taccctcctt taatcaagaa aattcgcttc gctcatgacc taaaacaatg
     3721 atgcgaattt tcttgttcgc tagggaaccc taggttccct tcgacggatt ctgcttcatt
     3781 aatgtttatt gcagaatcct gagcaccctc cttaaaccgg gaaggggaat cccctttcca
     3841 aaccccagta tagggacctt ttaggagatt tataaattcc tataccccaa gcaaaccata
     3901 tgtcaagccc tttgtaatgc agaattatat ttttatatta tcatagtttg gctgcatatg
     3961 taaagctgta aggtaaagga gggagcgggg ggagcttagg tgtttcggag ctagtgactt
     4021 aggagcttcg gtgtttcagc gtcaaagcag ttccggtcac atgctgtcat atcccgggcg
     4081 ttcaacggtc atgccgatat acaaaaacaa ggcttccacc ggagtgaaag ccttgtcttt
     4141 ttaacgtttg cagtatttta aaatgttaat cattatgttc tataatgcta tgttctataa
     4201 tgctatataa tgatttatat tattcctatt ttatttctta gaaatttgta ttattttata
     4261 ttagctttag cacatgcttc gttaatctct tcaataagtt tatcatatcc atttgcacga
     4321 taagaactaa ctatttcgtt ccaggttttt tctacattat cgcttaataa gcacttaatg
     4381 ataatttcac cggtccttgc agttgcctta tccttacttg gtacgtcaag gagaggtatt
     4441 cttaaatctg ttggaactgg ttttgcattt gcttgcagcc agtcatttaa atcactggac
     4501 attttctgga gttcaggatt tactgtagga gctgaccact ggcgtgtacc actccattca
     4561 gcaataaaac cgcatttaac aaatggatac gatactgata gaggaatatc ctttccttct
     4621 ccatcttttt gaggagtaag aacaatttta ccgtcttctt tatgccattc ctttccttca
     4681 ataccaaagt ggattaaatt gtagccttct tcatccagta catagtcaaa taatctcatt
     4741 actctgtcag ttttcttgtc atcacatttt gcattaatat aagtttctga ccatgccgga
     4801 ttgctaatgt gcctatagta atttccatct gaatgtttaa atggcttcaa aattgtaact
     4861 atgtctttat attttatgtt ttcgttaact ttttcaaatt gtgtctgtac atagccaagt
     4921 gttgaaggag ttacatcatg agcataagct cctgccttac ctgctgcaaa cttatctctg
     4981 ccttcttcac ctttaagagt tgctaaatct ctatccagtc cgcctgcatc ataaagtctc
     5041 tttaattctt tcattccagt taagcaatcc tttgtcataa aagcaggtat ccatttccct
     5101 ggattttcct tgtctcttac ccagcctcca ctttgtattc ctggttcata accaaggaaa
     5161 aaccaagtaa gccagtctga gttgtaacag gtcaagccga tagtatcctt tttattattt
     5221 ttatccgggt cattttctac aaatgctttc atcaatgcga tgaattcatc catattttca
     5281 ggttcttttg ttattcctac attttgcatc cagtcttttc ttagatgtat accggtatca
     5341 cagctccaat catctatatc aagatggttt agccttggta ttgcatagat tttgccatta
     5401 ggatcaccaa tggggtactt gtatatgtca aatccgggtg aattcataat tttattaatt
     5461 tgaggatatg gactcaaatc agatggaagt ggtctaacta taccttgttc tatccaattt
     5521 ttgtagtttg cggttcctat aacatcaata gcaaatatat caggtaattg tgaagatgct
     5581 gcccaaatct gtattttctg ggtataatca tcccatgtta cattcattgg cttaatggaa
     5641 atattgagct cattataaat tttttctctt actgcatcag gtgcttctgt tattgcctgc
     5701 ccaattcccc acaaagcaaa tgatatttca attttttcac ttaatggatt gctagcttct
     5761 gttttattgc tcgtctgcgc attagtgcct gtcggttcat ctttcccatt tttcttttcg
     5821 cagcctgcaa gcaagccaaa gatcatgcct actactaata aaatgacaat gactcttttt
     5881 acttttttca ttaactgtac ctccttgaaa tattttttat ataataacag ttttagactg
     5941 ttatttaccg gtaatagtat ttctagctat tatcagctat tatcagctat tatcagccat
     6001 tatcagccat tatcagccat tatcagctat tatcagccat tgtttagcca ttgtttagct
     6061 gctgtttagc tgctgtttag ctgctgttta gctgctgttt agccattgtt taactattcc
     6121 ttaactgctc caaataagat accctttgta aaatgcttct gtataaaagg ataaacgcat
     6181 aggatgggaa ctgtagcaac agtaattatg gccatctgca gggcttttgt atatgtaggt
     6241 ctgttattgc gtgctatttc agctcctata gggcttgcca ttatttgagc aaagtctagt
     6301 atagcttctc ttagtacaag ttgtaaaggg aaaagttttc tatttgaaat atatagtaaa
     6361 gctgtataat aatcattcca tctatctact gcataaaata atgaaattgt agcaattact
     6421 ggtgcagaca taggaagaac tattctatat aagatataaa ggtcgtttgc gccatctatt
     6481 ttggctgatt cttctatgct ggccggcaat tctttgaaaa aattctttag taatataagg
     6541 taaaaagaat ttattgctcc aggtattatc agaacccaaa ttttatctat taatccaagt
     6601 ttttgtattg ttaagtaata tggtataagt ccacctgaaa agaacatagg aaccaggata
     6661 aagttaaaca tcattttatt tccgggtacg aaactttttg aaagcgcgta cgctgatgca
     6721 gtggaaaaga tcatgcttaa aactgtgcct gtgactgtta taaatatgga aacaaaaaat
     6781 gaactcaaaa acatttctcc cttaaaaaca agcttatatg catcaagatt aatggatgtg
     6841 ggaaatatgt atattaagtg cttctgtact gctgcataat cggcaaatga tattattact
     6901 acataataaa aaggaaatat agttattact gcaagaatta tcataataat atagttaaat
     6961 gtatcaaaac catctttctt aattctatag ctggaatagt ccattctgaa tcctccttaa
     7021 taaattcctt tgccggtaat ttttttaaca gccaaattac ctgcaaataa cattataaag
     7081 tttactactg atttaaataa accaattgca gttgaagaag cgaaatcaag acctgtgata
     7141 aaagttcttc tgaaaatata tgtatctatt atatctgctt tatcgtaaac agctgcattg
     7201 tacatattaa atatctggtc aaagccggca ttcattatgt tggcgattgc cagtataagc
     7261 ataaccccta ttacggactt tatggcaggc caggtaatgt accgcataag ctgaaacctt
     7321 tttgcaccat ctacaatggc agcttcatac aactcaacac ttattcctgc tattgctgct
     7381 aaatatatta tagtacccca gccggcttct ttccaattgt tggtgatata aaggagtccc
     7441 ctgaaagtag aagcctgggt tagaaaatgg acctttttaa gacccaaagc agccagtatt
     7501 tcatttagaa cacctgcatc tcctagcaag ttgaagaata tacctgttac aacaatccag
     7561 gatagaaaat gtggaaatgt caataccgtc tgtataatgc gttttgtact atcttttctt
     7621 atttcattta gcaacaaagc aagtattatt ggaacaggaa attgaaatat tattctttgg
     7681 atacttataa ttattgtatt tgaaaaagct ttctgaaact ccatattact tagcaactca
     7741 tagaaatgtt ttaatccgtg catggatgtc catgagcttc cggtaattcc tttcgcaaag
     7801 ttgaggtctt taaatgccaa ttgtatccca tataatggta catatgaaaa tataaagtat
     7861 aatacaaacg taggaataaa cattatataa aaatacttac agtcaagtat ttgtttccaa
     7921 agcggcttgt tctttatggg tataatattg atgttttttt cgctaatttt agtattactc
     7981 attaagttta tacgctcctt ttccataact attagtcttt ccagataatc tggctttaca
     8041 ttccagatta acttcacttt tcagattagc ttcactttcc agattagctt cgcatttcag
     8101 attagctttg ctacaattat tatatagcaa ctctttgttt atgagtatgt aataggatta
     8161 gatattttcg gaatgttttt agaaatgtta ttttttgatc tatattattt ataacaatat
     8221 tataaaaaat atagaaaaat ggggacggtt ataacgaaac tagagaaccg tccccatgtc
     8281 tagagaaccg tccccatgtc tactacatca aaacttttag cataaccatc cttggccacc
     8341 agtcatcatt gctttccgag ctaaaaaatc catctgcttt tttactttgc agggtccagc
     8401 caatccattt ctccactttt tccagtaaag gtgaagtatg ttaagaatta ttagccgctt
     8461 ctgccgtttt ggggagttga atttaaaata actgatactt tcatgtcaac tctccgattc
     8521 ctttggtatt aggtttactg caaaatcctc cgcatttttc aattttatag ttcttgtagc
     8581 catatttaaa tgcctccaat aacttttgtt catataatgg gttttgtata atagcatcta
     8641 taccctcatt tacttccttc gcatattgat aacattgcat ggctgtatcg taattgatcc
     8701 attcaaaact atcgccggga tacctaatct caatataaaa atcatttaaa aacctgcatt
     8761 gcttagttaa ggattttaat tccgggaatg tttttaccag ttctcttaat aaggcgttta
     8821 aattatggct ttcacttagt cttgtttcaa acggtaaatc aaaggtaata aacaaatgct
     8881 tcaaaatttt ttcaactgct tgagtacaac ttgccgcaca attgttataa cagccaatat
     8941 cactgtccaa tttcgagtcg atgccgattt gggcaaaata taaatcactt actgcaatat
     9001 catattaact acctttcata caacaccatt ccttcccgtt caatgtcatt gactaccgat
     9061 atgcttattt ttttatttcg ttcaaaatcc atgcggttga agaaaacaat gtcatatggt
     9121 atattggtga cagattcctg ctctatcaac aagtcttcaa tttcatcaat aatattcaac
     9181 cgaaagtttc tgttagcagg tattttatca ttaacaataa cagcaaaatc tatatcactt
     9241 ttatctgtca tttttcccac agcacaactt ccaaacaaat atattttttc tatattttcg
     9301 ttttcagtga acttttccgt caataaaaaa ataaagaatt tataatcaaa aggaatatct
     9361 aatccgtcaa tttttgattt gattaacatt ttcacattaa cacttccttt taacgctcgt
     9421 cagttcctgt cctgttttca ttatctccag caatttcatc atatacttgt tgcgttacat
     9481 aaactttatc aaaaagattg cttaacagat ccaacttttc caaaaaagcc agccctatta
     9541 atggagtagt aattccttct ttttgtaata tgaaacttca gcgttctaag caaaacactc
     9601 catacaacgg gacactctta atattatttt caaatccaaa atttctggca gatatccgaa
     9661 ttgaatatgg cggtttatat aatgaaacaa aagcagcaag actttttgcc cgtacattat
     9721 cggcagattt tacctcaatc ggaattatat ttccctctct attttgtatt acaaaatcaa
     9781 gttctgcttt gccgggtgaa ctccaataat atggtacaaa tccatttacg tagaccatgc
     9841 agaacgctcc cttttttaaa tgggagatga atgcatgact accttatttc ccaaatctat
     9901 cgctgctaat ctattataag aaagaaaaaa ccaaagcaaa tgtcaaaaaa tccaaaaaaa
     9961 gtttttgaaa aagattgaaa ttttctcttg catatacaag caagatatgg tattatctaa
    10021 ttaaagaaca tttgtttgac aagggaggtg aaactattgg caactataac tttttctgtt
    10081 aagttaaaga aacctacaag agttaaacac agacagtggg ttgaagatca g
//
LOCUS       DAOSDD010000164        10071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2531_length_10071_cov_5.505192, whole genome shotgun sequence.
ACCESSION   DAOSDD010000164 DAOSDD010000000
VERSION     DAOSDD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_10071_cov_5.505192"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5617)
                     /locus_tag="PK733_13490"
     CDS             complement(<1..5617)
                     /locus_tag="PK733_13490"
                     /inference="COORDINATES: protein motif:HMM:NF023666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HOJ11589.1"
                     /translation="MKKRFLKSSILLIVLMLIFTLILSVNKTTIVSAYGYNINVLNNP
                     TPSSSSGWALTNAKWNDGSYGGEFKGLSNDPMYASQTFTFSDRDVYRMNNGEIKIDAS
                     GYFWAQITCSMWAYLKTTFYSASNTYLGEKIVEYGKYKTGNHNEKLSFTDYTVPKGTK
                     YIIYQAKNDDSLSGYHPSMRNFVLTVKDTTAPTYIATIPVTQPAKYKMGTKIRYKVQF
                     TEPVNVISQGYIKFKISTQEFNTSSNTNCTYAGQSDDGTTLYYDLTLPGTTTPGDNLS
                     VTITGLSGITVKDDAGNSCTTIPVTLNLSNGFYVDNKPPEVSSFTTTASTNALYKAGE
                     TLQFDVTFHEYIWVNGTPKINLSNGKSANYIKKTQTDTKIASFFYTIAKGEDKKNLAI
                     TSIDFTGIYDSVSNYATESAGYSSSAYNSFLNNKYISIDTIAPATTFSAVPGTPGSWQ
                     KAFDVTLSPTDNIAGVKEIYAAWTSVNGTPSYPSAANVDTSTNKVILPKNSGTYELYV
                     KLVDNVNNEDAQKSPYKYYFDFDAPVISASATKINGTDLVSSVTASVTDSHSGLEAST
                     YVWKNESNAAALTGNAGDGISIPQVDGVYTLTITAADKVGNISTKTIENLIVDSVAPQ
                     VAFVRTEGAAFQKSYTVDFAITDVKSGVAGYYYIWSTSATKPVIDDKAWVVTTETSLT
                     TPSGVSGTYYLHIKAVDNAGNIGIASTDGFNIDNKAPGVSITPNGNSGNEGKAGYDVV
                     INVSDEITLYDQLTIKYVLSDSEVLPAPSELMDLKGNSITIENLDKTKYLYIAATDEA
                     GNETVFKSNAFIADTAAPTGEISKATDIYYTNNNNVTININATDDYSSSIFMQIKIDN
                     AEGEWEDLAEQKTVSFNKVEGEHIIYARFKDVCGNVSEYKNVKYYYDVTPPQIELAYS
                     ATKLTNSSVTVTATAKDNVSDVSFETVSQKTFNENGSFEFIAVDRAGNRARAAAKVDY
                     IDKINPQISFASNEFDGKKHKSADITINASDANGISSLKYAVVRDGETAAGYISCTNG
                     QTIQITELNGTYTVSAIAYDGAGNSTTVSSQKIYLDNIAPVAGIIYSPSARTAQNVVA
                     SITFDEEVTITNNGGLNTYTFPDNGSFTFEFTDEAGNTGTAAATVAWIDRNPPRVSVT
                     LKNENGEALNAEKWTNKDVIVEINIPADCFIEVLKFNNISHEYSDYVTDLGNNQYKVS
                     GYGRLFYQVCDSITGTIASNEVVIRIDKNPPTITNVTYSTTDWTNMNVTATVSATDDY
                     NDVVYVNGSSHVFTENSEFGFIIDDSLGNRTTKTVKVSNIDKDVPVAKVSYDNTALTN
                     QNVTATVSFDEGGSPVSITNNNGLNTYKFTANGEYTFEFSDKAGNAGSIKAAVNNIDK
                     TPPEISLISDDFDLKPHKSASVKILGTDINGINSIKYRFVKEGESASYKSCENGDTVT
                     LDNADGTYGDGIYSDGTYTLEVTAYDNAGNEKRVASPSLILDNTAPMANISYAPSART
                     AQNVVASINFNEDAVITNNNGLSEYTFTDNGDFTFEFSDIAGNTGTKTASVTWIDRNQ
                     PVATVDLSNENWTNEDVTVTLTPQDGAAIQNVKFNDEEIAETAENQYAICEYGILTYE
                     VLDLTTETVGKGEVLVKIDRTEPTVTNITYSTKNWTNKDVSVNITAADNLSQVIYENG
                     SSYTFTENGSYDFIIKDSAGNKAVKTVTVDWIDRDVPVAQVNYNITHLTNQNVIATIS
                     FDDGGSPVAITNNNCSRIYEFTANGEFIFEFSDEAGNKGTAAASVSNIDKVLPEISFS
                     SSYFDMSMYTSVSVTINATDLNGIADLKYKFIKAGEDGGEFAAIQNGDTVALDSVSGV
                     YQVIAIAKDTAGNEKTAYSQSLYLDNSAPV"
     gene            complement(5622..6674)
                     /locus_tag="PK733_13495"
     CDS             complement(5622..6674)
                     /locus_tag="PK733_13495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOJ11590.1"
                     /translation="MKKFIIVLVCAMLCINTVCVAEEEYGCECGYGYGYEYVSGYGVR
                     VGIIDSGVNKTNENVLTGFNYLHNNTDTTDNKGHGTAISDIILEIAPNVKIIPLKCTE
                     LNQITDNSAVIAAIYKAVDDFKCDVINISIGMPHSGELKAAVDYAANKGAIIVSAVGN
                     DGELNYKQSKVYYPAGYENVIGVGSVDENNLVSSFSQRNESVFVVTSSKEPGTSFAAA
                     KISGMAAIAKSFNKRMSVWLFMKYLKRASIDFGDKGYDTSYGNGALNIELLLNNLKND
                     NPVYITYLDEQKFTLYNSKKETTKGILIFMGEHVTSEEIEILPNREKELSLRNSESCF
                     FWQKSIYDLIWIKKGE"
     gene            complement(6676..7077)
                     /locus_tag="PK733_13500"
     CDS             complement(6676..7077)
                     /locus_tag="PK733_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11591.1"
                     /translation="MAIVNEPNILRFYGKRDEYMEYQFYLKPNYTNCMTERNSRSRIT
                     YYTNKKMEEMYPEGGYRIIGEIGNFARKYAKQDVIITDTGKRIPIFPRGSLKKPVEWV
                     AGCAAVEENTYVSVVKSIIPHWLRILTYKRV"
     gene            complement(7216..9144)
                     /locus_tag="PK733_13505"
     CDS             complement(7216..9144)
                     /locus_tag="PK733_13505"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ11592.1"
                     /translation="MLKEIKREWFLILGFVLTVIFMNLPAVYTYYYHDISGAPKAINA
                     RMDLSAANPADGKIYLDGEWEFYWKRFIASEPDQLYIPDLIIKVPDSWSKYRIERGSL
                     SAEGFGSYKLTMTGLEYDNAVTLFIPDFGGAYRAFIDGQLAAESGIVSKDTKKIFTTP
                     KADLHPLVLSNRMVHEIVIEVANARFSGLYMTPVLWDYNQIIRENSFRNAVRFILFGI
                     VLFSLLSLLIMYIVTIRRKLHSIWLPVMVIFILMRVMLTSEFYSLWQPVLFLNLSYES
                     TNELMYFTTFVLKYLLIFLVQEQCGIEFNKREKMYFLIHYIFLYLIYLLVPKSIYNNY
                     LSLLIPMLTYVLDLYIFFKIYRGRHTMKKFGMVVFWDIIIVIIGLTLDSYYINGKIYI
                     DMSLILLILFALFALIMIFVYAMRSVDLYDDFTISSSRLKLANNQIAMQKEYYDTLSR
                     QMNEIREMKHDIRHFIRAMSRLADENKFDELRVFLGEYNEKTDREQLPIFCENTIANS
                     IIGYYYLRAKESGIFFESFCSINRQSVMSDSDLCIVLGNALENSVYACEQMENHEKMY
                     ITIKAGTMKKQMLIKLTNSYSGNVEARDGHLISTKSGKSHGLGIRNIERVVASYGGIV
                     KIEYNEKEFILSVAVPEK"
     gene            complement(9149..9868)
                     /locus_tag="PK733_13510"
     CDS             complement(9149..9868)
                     /locus_tag="PK733_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ11593.1"
                     /translation="MPIQIGICDDRGEDIKELTEALYSYDPSFQISTYSDGESLLDDF
                     HDSKILFDILFLDIYMPGMNGIETAGQIRASMKDAKIIFISSSNEHYREAYDVFAFNY
                     INKPLNREKLNRILDQALLDIIGERRQQISFSYKGSVYRIFCRDILYIESNDKIICFY
                     MADKRILRCYAKLDEILKQLPEELFIRCHQSFAVNIFHVTEMAENHFHIDPAIISISK
                     KYQKIAKDKYFQYLFTHMNRG"
BASE COUNT         2892 a         2036 c         1642 g         3501 t
ORIGIN      
        1 caacaggtgc agagttgtca agatacaggc tctgactgta tgctgttttt tcgtttcctg
       61 cagtatcttt agcaattgca attacctggt atacaccgga tacactatca agtgcaacag
      121 tatcaccatt ttgaattgct gcaaattcgc caccatcttc accggctttt ataaatttat
      181 attttaaatc tgcaatacca tttaagtcag ttgcattaat agtaacactt accgatgtat
      241 acatactcat atcaaaatat gaagagctaa atgatatctc aggtaaaacc ttatcaatat
      301 ttgaaacact tgcagcagca gttcctttat ttccggcctc atcgctgaat tcaaatataa
      361 actcaccgtt tgcggtaaat tcatagatgc ggctgcagtt attgtttgtt attgcaacag
      421 gtgaacctcc atcatcaaaa cttatagttg cgataacatt ttgattagtt aaatgtgtta
      481 tattatagtt tacctgtgct acaggtacat ccctatcaat ccagtcaact gtaacagttt
      541 ttactgcttt atttcctgca ctgtctttta ttataaaatc atagctgcca ttttcagtaa
      601 atgtgtaact gctgccgttt tcataaatca cctgacttaa attgtcagca gccgtaatgt
      661 ttactgatac atctttgtta gtccaattct ttgttgaata tgttatattt gtaactgttg
      721 gttctgtacg gtcaatcttt actaaaactt caccttttcc aacagtttca gttgttaaat
      781 ccaaaacttc gtaagttaat ataccatact cacaaattgc gtattgattt tcagcggttt
      841 ctgcaatttc ttcatcatta aatttgacat tttggatagc cgctccatcc tgaggagtta
      901 atgttactgt aacgtcttca tttgtccagt tttcattgct taagtctaca gttgccacag
      961 gctggtttct gtcgatccag gttactgatg cagtttttgt tcctgtattt cccgcaatat
     1021 cactaaattc aaatgtaaaa tcgccgttgt cagtaaaggt atactcactt aaaccgttat
     1081 tatttgtaat tacagcatct tcattaaagt taatacttgc aacaacgttc tgtgcagttc
     1141 tggctgaagg cgcataggat atattggcca tcggagcagt gttatctaaa atcaggcttg
     1201 ggcttgcaac cctcttttca tttcccgcat tatcatatgc tgttacttcc aatgtatatg
     1261 ttccatcact gtatatccca tcaccatatg tcccgtcagc attgtctaat gttactgtat
     1321 caccattttc gcaagactta taacttgcgc tttcaccttc ttttacaaat ctatatttta
     1381 tgctattaat cccgtttata tctgtaccca gtattttaac acttgcactt ttatggggct
     1441 taaggtcaaa atcatcagat atgaggctta tctcgggcgg ggttttatca atgttgttaa
     1501 ctgctgcttt gatgctccct gcgtttcctg ctttatctga aaattcaaat gtgtattctc
     1561 cgtttgcagt aaacttgtaa gtatttaatc cattattgtt tgttatgctt actggtgaac
     1621 cgccttcatc aaaggataca gtcgctgtta cgttttgatt tgtcaaagca gtgttatcgt
     1681 agcttacttt tgcaacaggg acatccttgt ctatatttga aacctttaca gttttggttg
     1741 tgcggttgcc aagactgtca tctattataa acccaaactc actattttcg gtaaagacat
     1801 gactgcttcc atttacataa accacatcat tataatcatc ggttgcacta acagtagcag
     1861 ttacattcat gtttgtccag tcggttgttg aatatgtgac atttgttatg gttggtggat
     1921 ttttgtcaat tcttataact acttcattag aagctattgt accggttata ctgtcacaga
     1981 cctgataaaa aagccttcca tatccgctta ctttatactg attattgcct agatcagtta
     2041 cataatctga atattcatgg gagatattgt taaactttaa cacttcaata aagcaatcag
     2101 caggaatatt gatttctaca atcacatctt tattggtcca tttctcagcg tttaaagctt
     2161 ctccattttc gtttttaagt gttacagaaa ccctgggagg atttctgtca atccatgcaa
     2221 ccgttgcagc agcagttcct gtgttccctg cttcatcagt aaattcaaag gtaaagctgc
     2281 cattatccgg gaaggtatat gtatttaaac ctccattgtt tgttatagtt acttcttcgt
     2341 caaatgttat acttgcaaca acattttgtg cagttcttgc agagggggag tatataattc
     2401 cggcgaccgg cgctatgtta tcaagataaa ttttctggct tgaaacagtt gtactgtttc
     2461 ctgctccatc ataagctata gcactaactg tgtatgtacc attgagttct gttatctgaa
     2521 tagtctgacc gtttgtacag cttatataac cagctgcagt ttcaccgtct ctaacaactg
     2581 cgtattttaa actgcttatt ccatttgcat cacttgcatt aattgtaata tcggctgatt
     2641 tatgcttttt accgtcaaat tcattagacg caaaagaaat ctgcgggttt attttgtcaa
     2701 tatagtcaac ctttgctgcg gctcttgctc tgttccccgc tctatctacc gcaataaatt
     2761 caaaacttcc gttttcgtta aaagtctttt ggcttactgt ttcaaaactt acatccgata
     2821 cattgtcctt tgcagtcgct gttacggtaa cagagctgtt tgttaactta gtagcagaat
     2881 atgcaagctc aatttgaggc ggagtaacat cataataata tttaacgttt ttgtactcag
     2941 aaacatttcc gcaaacatcc ttaaatcttg catatattat atgttcacct tcaactttat
     3001 taaaagaaac ggttttttgc tctgcaaggt cttcccattc accctctgca ttatcaattt
     3061 taatttgcat aaaaatgctg ctggaataat catccgtggc attaatatta attgttacat
     3121 tattattgtt tgtataataa atatcagtcg cttttgaaat ttcaccagtt ggagcagcag
     3181 tatcagcaat aaaggcattt gacttaaaca ctgtttcatt tcccgcctca tctgttgctg
     3241 ctatatacag gtatttggtt ttatcaaggt tttcaattgt aatgctgttc ccctttaaat
     3301 ccataagctc cgaaggtgca gggagcactt cactgtctga aagtacatat tttatagtca
     3361 attggtcata aagggttatc tcgtcactta cattaattac tacatcgtaa cctgcttttc
     3421 cttcattgcc tgaattaccg tttggtgtaa tagatacacc aggagccttg ttatcaatat
     3481 tgaagccatc cgtgcttgca attccaatat ttccggcgtt gtctacagct tttatatgca
     3541 gataatatgt tccggaaaca cctgatggag ttgtaaggct tgtttcggta gtaactaccc
     3601 atgctttatc atcaatgaca ggctttgttg cagaagtact ccagatgtaa tagtagcctg
     3661 caacacctga ttttacatct gttatagcaa agtccactgt ataacttttc tggaaggctg
     3721 ctccctctgt ccggacaaaa gcaacttggg gtgcaacact gtctacaata agattctcaa
     3781 ttgttttagt gcttatattg ccaaccttat ctgctgcagt tatagttaat gtgtaaacac
     3841 catctacctg gggaattgaa atcccatcac ctgcattacc tgtaagtgca gcggcattgc
     3901 tttcattttt ccacacataa gtagatgcct caagccctga gtgtgaatct gttaccgacg
     3961 ctgtaactga gcttaccagg tccgtaccat ttattttggt cgcgcttgca ctgattacgg
     4021 gagcatcaaa atcaaaatag tatttgtatg gtgatttctg tgcgtcttca ttattaacat
     4081 tatctaccag tttaacatac aattcatatg ttcccgaatt tttaggtaat attaccttat
     4141 ttgtacttgt atctacatta gctgctgaag gatatgaagg agtaccattg acggatgtcc
     4201 atgcagcata tatctccttc acacctgcaa tgttatcagt tggtgaaaga gtaacatcaa
     4261 aagctttctg ccagctgcct ggtgtgcctg gtacggctga aaaagttgtt gctggagcta
     4321 ttgtatctat gctgatatat ttattattta aaaagctgtt atatgcactg gaactataac
     4381 ctgccgattc tgttgcatag tttgatacac tatcataaat gccggtaaaa tcaattgagg
     4441 tgattgccag atttttttta tcctctccct ttgctatggt gtaaaagaag cttgctattt
     4501 tagtatcagt ttgggtcttt ttaatatagt ttgcactttt tccattgctt aggtttattt
     4561 tgggtgttcc attaacccat atatattcat ggaaagtaac atcaaattgc aaggtttctc
     4621 ctgctttata cagtgcattt gtacttgcag ttgtggtaaa gcttgaaacc tccgggggct
     4681 tattatctac ataaaacccg ttagataggt ttagtgttac cggaattgtg gtacaagaat
     4741 ttccagcatc atctttcaca gtaataccgc ttaaaccagt aattgtaaca gacaaattgt
     4801 ctcccggtgt tgttgtaccc ggcaaggtta aatcataata aagtgttgtt ccatcatcac
     4861 tttgtcctgc gtaagtacag tttgtattgc tgcttgtgtt aaattcctgt gtacttattt
     4921 taaatttaat ataaccctgg ctgataacat taacaggttc tgtaaattga actttatacc
     4981 ggattttcgt acccatctta tattttgcag gttgggttac aggaatagtt gcaatatatg
     5041 tgggtgctgt ggtatctttt actgtgagca caaaatttcg cattgaagga tgatatcctg
     5101 aaaggctgtc atcgtttttt gcttgatata taatatattt tgtgcccttt ggcacagtgt
     5161 agtcagtaaa tgacagtttt tcattatggt ttcccgtctt gtatttgcca tattctacta
     5221 ttttttctcc caggtatgtg tttgatgcac tataaaaagt tgtttttaaa tatgcccaca
     5281 tggagcatgt aatctgagcc caaaaatatc cactggcatc tatctttatt tctccattat
     5341 tcatacgata tacgtcgcga tctgaaaatg taaatgtctg acttgcatac atgggatcat
     5401 tggagagtcc tttaaactca ccaccataag aaccatcatt ccacttagca tttgttaatg
     5461 cccaaccact tgagctagat ggagttggat tgttgagcac attgatgtta tagccatatg
     5521 cgctaactat ggttgtcttg ttaacagata aaattaatgt aaaaattaac attaatacaa
     5581 tcagcaagat actgctcttt aaaaacctct ttttcatatt atcattcccc tttcttgatc
     5641 cagatcaagt catatatgct tttttgccaa aaaaagcaac tttcagaatt tcttaaactt
     5701 agttcttttt ctctatttgg caaaatctca atttcttcgc tggtgacatg ctctcccata
     5761 aaaattaaaa tacctttggt ggtttctttc ttactgttat acagcgtaaa tttctgctca
     5821 tccaggtaag taatatatac cggattgtca tttttaagat tgtttaataa aagctcaata
     5881 ttcaaagctc catttccata gcttgtatca tagcctttat cgccaaaatc aattgatgct
     5941 ctctttaaat acttcataaa aagccatact gacattcgtt tgttgaagct ttttgctatt
     6001 gctgccatac ccgatatttt agctgctgca aagcttgtgc ctggttcttt actggatgtt
     6061 acaacaaaaa ccgattcgtt cctctgagaa aagcttgaaa ccagattatt ttcatcaact
     6121 gaaccaacac caattacatt ttcatagcct gccggataat aaactttgct ttgcttataa
     6181 ttcagctcgc catcatttcc taccgccgat acaataattg ctcctttatt tgctgcataa
     6241 tcaacagcag ctttaagctc acctgaatgg ggcattccta tacttatatt tataacatcg
     6301 catttaaagt catctactgc tttataaata gctgcaataa cagctgaatt atctgttatc
     6361 tgattaagct cagtgcattt aagcggaatg atttttacat ttggtgcgat ttctaatata
     6421 atgtccgaaa tagcagttcc atgaccttta ttatcagttg tgtctgtatt attatggagg
     6481 taattaaacc cggtcaagac attttcattt gttttattta ctcctgaatc aataattccc
     6541 acacggacac catacccgga aacatattcg taaccatatc cgtaaccaca ttcacaacca
     6601 tattcttcct ctgccacaca aactgtgttt atacatagca ttgcgcatac taatacaatt
     6661 ataaatttct tcatactaca ccctcttata tgtaagaatt cgtaaccagt gtggaataat
     6721 acttttcact acagaaacat aggtgttttc ttccacagct gcgcagcctg caacccattc
     6781 aacaggcttt ttcaagctgc ccctcgggaa tatggggatt ctttttcccg tatcagttat
     6841 aataacgtcc tgtttggcat acttccttgc aaaattaccg atttctccga taattctata
     6901 accaccttcc ggatacattt cctccatttt tttatttgta taatatgtta ttctactgcg
     6961 gctgttacgc tctgtcatgc aattagtata attaggtttt aggtaaaact ggtattccat
     7021 atattcatct cgctttccgt aaaaccgcaa tatattgggc tcattaacga tagccactgg
     7081 tatatattcc ctcaaatttt aaaaatgtgc ctcaatacat cttattttat attatagaga
     7141 aaaaatttaa cgaattatgt caatggtgaa atcgaccatt tttttgccga aattggcatg
     7201 aaaagaaaag gtaatttatt tttctggtac agccactgaa aggataaact ctttttcatt
     7261 atactctatt tttactatgc ctccgtatga tgctaccacc ctctcgatat tccgtatacc
     7321 aagaccgtga gactttccgc tctttgtaga gataagatgc ccgtctctgg cttcaacatt
     7381 gccgctatag gaatttgtca actttatgag catttgtttt ttcattgttc ccgctttaat
     7441 ggttatatac attttttcat ggttttccat ttgttcgcat gcgtatacgg aattttccag
     7501 agcatttcca aggacaatac agaggtcact atcactcata acagactgcc tgttgatgct
     7561 gcaaaagctt tcaaagaaaa tcccgctctc cttcgcccgc aagtaataat atccgataat
     7621 ggagttagct atagtattct cacagaatat cggtaattgc tctctatcag ttttttcgtt
     7681 atattcacct aaaaataccc tcaattcatc aaatttattc tcatcggcaa gcctgctcat
     7741 tgctctgata aaatgacgga tatcatgttt catctcccgg atttcattca tctgtctgct
     7801 cagtgtatca taatattcct tctgcattgc aatctggttg tttgccagct tcaatcttga
     7861 ggaggatatt gtgaaatcat cgtagaggtc aacgctgcgc attgcataaa caaaaatcat
     7921 tattaatgca aacaaagcga ataaaatcag aagaattaag gacatgtcaa tgtatatttt
     7981 tccattgatg taataactgt ccaatgtcag tcctattata acaatgatta tatcccagaa
     8041 aactaccata ccaaatttct tcatagtatg ccgtccacgg tagattttaa agaatatata
     8101 gagatccaaa acataggtga gcatgggtat aagtaatgat aggtaattgt tataaatgct
     8161 cttcggcaca agcaaataga tgagatacag aaaaatataa tgtatcagga aatacatttt
     8221 ctcgcgcttg ttaaattcaa tgccacactg ttcctgcaca aggaaaatca gcaaatattt
     8281 caggacaaag gttgtaaagt acattagttc atttgttgac tcataggaga ggtttaggaa
     8341 caaaacgggc tgccataaac tgtaaaattc cgatgtcagc ataacacgca tcagaatgaa
     8401 tatgaccata accggaagcc atatggaatg tagttttcgc cggatagtca ctatatacat
     8461 tatgagcagg cttaaaagcg aaaacagcac tattccaaac aaaataaacc gaactgcgtt
     8521 tctgaagctg ttttctctaa taatttggtt ataatcccac aggacaggcg tcatataaag
     8581 tcctgaaaaa cgtgcatttg caacctctat tactatttcg tgcaccattc tattagaaag
     8641 taccaaaggg tgtaaatctg ctttcggagt cgtaaatatc tttttggtgt ctttagaaac
     8701 aataccactt tcagcagcaa gctgcccgtc gatgaatgct ctatatgctc cgccaaagtc
     8761 cggtataaac aatgtaacag cattgtcata ctcaaggcct gtcatggtga gtttataact
     8821 tccgaaccct tccgcagata aactacctct ctcaatccta tattttgacc atgaatccgg
     8881 caccttaatt attaaatccg gaatataaag ctgatcaggt tctgacgcaa taaagcgttt
     8941 ccagtaaaat tcccactctc catccaaata aatcttcccg tcggccggat tggcagctga
     9001 caaatccatt ctggcattta tagccttagg tgcaccgcta atatcatgat aataatatgt
     9061 atatacagcc ggtagattca taaaaatgac tgtcagaaca aaacccagaa taagaaacca
     9121 ttcacgctta atttctttta acattttact atcctctatt catgtgagtg aaaagatact
     9181 ggaaatactt atctttcgct atcttttggt acttttttga tatactgata atagctggat
     9241 caatatgaaa atgattttca gccatttcag ttacgtggaa aatatttaca gcaaagctct
     9301 gatggcatcg gataaataac tcttccggca gttgtttcag tatctcatcg agtttggcat
     9361 aacaccttag tattctctta tctgccatat aaaaacatat gatcttatca ttactttcta
     9421 tatatagaat gtcccggcaa aaaatccggt acactgaacc tttataactg aaactgattt
     9481 gctgccggcg ttcacctata atgtccagca gcgcctgatc caatatgcgg tttaattttt
     9541 cccggtttaa cggtttgttt atgtaattga atgcaaatac atcatatgct tcccggtaat
     9601 gctcattact tgaagatata aaaatgattt ttgcatcctt catactggca cgtatttggc
     9661 cagcggtttc aatcccattc attccaggca tatatatatc caagaaaagg atatcgaaca
     9721 ggatttttga gtcatgaaaa tcatcaagca aagattctcc atcggaataa gttgaaatct
     9781 ggaaagaagg atcatatgaa tacaaagcct ccgttagttc cttgatatct tcccctctgt
     9841 catcgcaaat gccaatttga atgggcataa aaaagatcct ccctttaaac cacaaataca
     9901 ttttcagaaa cttttaattt catatctcta tctttatctt taatggtgct aatctatatt
     9961 tatatatttt attcacccaa tacagtaaag tcaatcagtt tttcttaatt agggcacatt
    10021 ttcttcctat gatacaaaat atggcagcat acgatttccc gagtataaaa t
//
LOCUS       DAOSDD010000165        10066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2535_length_10066_cov_4.721207, whole genome shotgun sequence.
ACCESSION   DAOSDD010000165 DAOSDD010000000
VERSION     DAOSDD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_10066_cov_4.721207"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PK733_13515"
     CDS             <1..620
                     /locus_tag="PK733_13515"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOJ11594.1"
                     /translation="IGVNGFKNIGGNIKQFISLRYGQVEKSILQKCPYIKHATVKYLP
                     PNKIMIQVEERTPVALIPYIGAFLLVDREGYILDTATGDEGILLPKIKGLNFTSYQIG
                     QALNIENRDKFDELIILVDTLTQEDKNDNFKIVEKINTIDISDSNNLYLFIDSRIIVN
                     LGSLDDLNYKIRSLHQIFFKNIGEGEKGFSDYTAGDYPVFIPGGG"
     gene            675..1031
                     /locus_tag="PK733_13520"
     CDS             675..1031
                     /locus_tag="PK733_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HOJ11595.1"
                     /translation="MIPIIGLIVGIIAGVFIPYNIPQQYSNYVAVAILAALDSVFGGV
                     SATLQGNFDMKIFLSGFFGNALLAAGLAYIGDQLGIQIYLAAVFAFGNRLFLNFAIIR
                     RSILNRRTKKDNIKER"
     gene            1082..1957
                     /gene="ftsA"
                     /locus_tag="PK733_13525"
     CDS             1082..1957
                     /gene="ftsA"
                     /locus_tag="PK733_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOJ11596.1"
                     /translation="MEDIIVGIDIGTTKVCTLIGKVNRTGQMEIYGKGCIPCDSVKKG
                     VIIDIEAVSKAIRHSVEQAENSSGMRIGSAYVNIYGTHVSVLNHIDSIEVSSENRLIT
                     TNELDRLYNQIEHISIPEGSQIIDIIPRQYVIDGYDGIKDPLGMSGLNLEIDADIVVG
                     KLTSVQNIIKSVEKAGLEVDGIIIESFATSELALTSEEKEMGVVLIDVGGGITDVSAF
                     KNGKVLMYDSIPVGGDHITNDISIGLHISYSEAEKIKKQYELALTTLIKNDQEISVYD
                     VNDEITRNIKVSQVI"
     gene            2003..2323
                     /locus_tag="PK733_13530"
     CDS             2003..2323
                     /locus_tag="PK733_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11597.1"
                     /translation="MICKYEDLGEFDSGIVLTGGGISYVNGNRQIAGEVFGLPVRVAS
                     YRNYAGISKPEFAAAAGIIKYVSSRNKRASIVYNVKDKQVETEHKKDGIFNRIIKVLE
                     RWFF"
     gene            2552..3649
                     /gene="ftsZ"
                     /locus_tag="PK733_13535"
     CDS             2552..3649
                     /gene="ftsZ"
                     /locus_tag="PK733_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ11598.1"
                     /translation="MLEFDVDMEQFAQIKVIGIGGGGNNAVNRMINAGLRGVEFISIN
                     TDKQALFLSKANTKIQIGEKLTKGLGAGANPDIGEKAANESKDEISQAIKGADMVFIT
                     AGMGGGTGTGAAPIVAQVAKEMGILTVGVVTKPFMFEGRKRMQFAERGIENLKASVDT
                     LVTIPNDRLLQVAEKKTSIVDAFRMADDILRQGVQGISDLIIVPGLINLDFADVKTIM
                     LNTGLAHMGIGRASGDGRAEEATKKAIESPLLETSIEGAKGVLLNITGGADLGLFEVD
                     VAAGLIEKSADPDANIIIGAVIDENLEDEILITVIATGFEQGPIIRKQEKTTTPEKPS
                     KPSYAEKAPPAIDINNDELDIPTFLRRNRYK"
     gene            3987..4883
                     /gene="spoIIGA"
                     /locus_tag="PK733_13540"
     CDS             3987..4883
                     /gene="spoIIGA"
                     /locus_tag="PK733_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOJ11599.1"
                     /translation="MEIYLDVLILENVVMNYLILWTTGKILKTRTSNIRLFMGALIGA
                     AYAAFLVIKPGLGTYFSATATVILSIAMVAVTFSPEKITAFFKTLVVFYVSTFVFAGA
                     TFAFFYMNNKGTIVRNGLLYVFWESKWTTVVLSIITSIIILNIFLEVIQQKYFKEKLI
                     TRLKVIFDSKEIDMSALVDTGNSLQDPLTNMPVIVVEFTAIKEILPSEICNIFEGMHE
                     YDLSSLSSRLYDSKWFNRFRLIPFNSLGKENGMLIGFKPDYVVIGEHTDKKGVSDVII
                     AIYNKVLSKGKDYKALLGPDLL"
     gene            4922..5653
                     /gene="sigE"
                     /locus_tag="PK733_13545"
     CDS             4922..5653
                     /gene="sigE"
                     /locus_tag="PK733_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOJ11600.1"
                     /translation="MIKFKHYKLILYLKYIKVLERFGLYNGRHIHYIGGSEALPPPLS
                     DDEESCLLDQLGKSDYGVKKILIERNLRLVVYIARKFENTGVGVEDLVSIGTIGLIKA
                     INTFKPSKNIKLATYASRCIENEILMYLRRNNKTRTEISIDEPLNIDWDGNELLLSDI
                     LGTDNDIIHRSLEDEIDKELLNAALMKLSSREKKIMELRFGLVNGVEKTQKEVADMLG
                     ISQSYISRLEKRIINRLRKEINRMV"
     gene            5842..6615
                     /gene="sigG"
                     /locus_tag="PK733_13550"
     CDS             5842..6615
                     /gene="sigG"
                     /locus_tag="PK733_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOJ11601.1"
                     /translation="MHINKVEICGVNTSKLPVLTNEQKKVLFERMHKGDDSAREEFIQ
                     GNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLIKAIDNFDVSHNVKFSTYAVPMIIGEI
                     RRYLRDNNSIRVSRSLRDVAYKALQVKEKLTNKNSKEPTIDEIAKELEVQKEDVVLAL
                     DAIQDPISLFEAVYHDGGDAIFVMDQVSDEKNTDENWIEEISLKEAMQKLSDREKLIL
                     NLRFFEGRTQMEVAEEIGISQAQVSRLEKTALLHMKKYI"
     gene            6738..6998
                     /locus_tag="PK733_13555"
     CDS             6738..6998
                     /locus_tag="PK733_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOJ11602.1"
                     /translation="MNRTNDLKQKEVINISDGRRLGFVNDVEIDLECGKIEAIVIPGG
                     GRLFGLIGKDNEFVIPWERIRKIGEDIILVDLDDRFIKKYFT"
     gene            7022..7480
                     /gene="nrdR"
                     /locus_tag="PK733_13560"
     CDS             7022..7480
                     /gene="nrdR"
                     /locus_tag="PK733_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOJ11603.1"
                     /translation="MKCPYCSYEEDKVIDSRPTDEGTAIRRRRECLKCQRRFTTYEKI
                     ETIPIMVIKKDRSRQPFSREKILNGLMRASEKRPVSVADLENIVNEIESHIYNSTQKE
                     ITSKEIGEMVMGRLKNLDEVAYVRFASVYRQFTDLNNFMNELRKILEEKK"
     gene            7628..7963
                     /locus_tag="PK733_13565"
     CDS             7628..7963
                     /locus_tag="PK733_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ11604.1"
                     /translation="MFGVTEQKIEKWKSKNKVDKIVEAMNHKDKVIRLAAIKAGAGMK
                     DEKIFNALVIFLNDPDPLIRAGSVDSLGSLGIARAQDHLRFVSLNDSDENVREKALEA
                     MKNIIAKKM"
     gene            8293..9852
                     /locus_tag="PK733_13570"
     CDS             8293..9852
                     /locus_tag="PK733_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOJ11605.1"
                     /translation="MLSKIKSCGLMGIDGFIINVETDISNGIPSFEIVGLGDIAVKEA
                     KERVRTAIKNARYEFPVKRITVNLAPANLRKEGSALDLPIAMGILACTGQIDSSLVKD
                     CIFIGELSLDGDIKGVNGVLSMALCAYHEGIGNIILPADNADEAAVVEGLNVFPAKNI
                     SDIVLHLNGHLRIKKHVIEIRNIFCNDSDNNSDNDNDNEISGDDFCEVCGQENVKRVL
                     EIAAAGFHNLMMVGPPGSGKTMLARRLPSILPPLTFDEALEVTRIYSIAGILPTKTPL
                     ITARPFRSPHHTISTAGLVGGGKYPKPGEISLSHYGVLFLDEIPEFEKHTLEVLRQPL
                     EDEIVTISRLSASITYPAKTTLILAGNPCPCGYLTDETKKCTCTYNQIQNYMGKLSGP
                     LMDRVDLYVEAAPVKYKDLDSNPCGEKSKDIRQRVIKARNIQLERYKNNKIFSNSQLN
                     TGLIKKYCRLDNQCSLIMKNAFEKFGLSARAYNRILKVARTIADLDESENVRAYHLAE
                     AIQYRFTKAWK"
BASE COUNT         3625 a         1346 c         2117 g         2978 t
ORIGIN      
        1 caataggagt aaacggcttt aagaatattg gcggcaacat taaacaattc atatcattaa
       61 ggtatggaca ggtagaaaaa agtattttgc agaaatgtcc atacattaag catgcaactg
      121 taaaatattt acctccaaat aaaataatga ttcaagtgga ggaaaggact cctgttgcat
      181 taattccata tataggtgct ttcctgctgg ttgacaggga aggatatata cttgatactg
      241 caactggaga cgaaggaatt cttttgccga aaatcaaggg attaaacttc acaagctatc
      301 agattggaca agcactcaat attgaaaata gagataaatt tgatgaactt attattttag
      361 tagatacatt gactcaggaa gataaaaatg ataactttaa aattgtagaa aagattaata
      421 caatagatat tagtgatagc aataatttat atttatttat agattcaaga ataattgtta
      481 atcttggtag tttagacgat ttgaattaca agattagatc gttgcatcaa atatttttta
      541 aaaatattgg tgaaggtgaa aaagggtttt cagactatac agctggtgat tatcctgtat
      601 ttatacccgg aggcggataa aagccgcgaa gatgcattaa tacttgatat taccgtgtag
      661 atatggaggt tattatgatt ccaataatag gattaattgt aggaataata gctggggtat
      721 ttatcccata taatattcca cagcaatatt caaattatgt tgctgttgca atacttgcag
      781 ctcttgatag tgtatttggc ggggtctcag ctacattaca gggaaatttt gatatgaaaa
      841 tttttctatc aggttttttt ggcaatgcat tactagcagc aggacttgca tatattggtg
      901 accagctggg tattcaaatt tatttagctg ctgtttttgc tttcggcaac agattatttt
      961 taaatttcgc aataattaga agaagtatat tgaatagaag gacaaaaaaa gataatataa
     1021 aagagagata gtatatcatg ggaaggtaac tttattttaa cttccgacag ggggaaaaaa
     1081 agtggaggat ataattgttg gtatcgatat tggtactacc aaagtttgta ctttaattgg
     1141 aaaggttaat aggacagggc agatggaaat atacggtaaa ggctgtatac cctgtgatag
     1201 tgtaaaaaaa ggagtaatta ttgatataga agctgtatcc aaagcaatca gacattctgt
     1261 tgaacaagcc gagaatagct cgggaatgag aatcggatca gcatatgtta atatatatgg
     1321 tactcacgta agcgttttaa atcatattga tagcattgaa gtttcaagtg aaaacaggtt
     1381 aattacaacc aacgaacttg acagactgta caaccaaata gaacatattt caattcctga
     1441 aggcagccag attattgata ttattccaag acaatatgtt attgatgggt atgatggtat
     1501 aaaggatcct cttggtatgt cagggcttaa tctggaaatt gatgcggata ttgttgtagg
     1561 aaaactcact tccgtgcaaa atataataaa aagtgttgaa aaagcaggcc ttgaagttga
     1621 tggcattatt attgaatcct tcgctacaag tgagttggct ttaacatctg aagaaaagga
     1681 aatgggtgtt gtcctcattg atgttggcgg tggtattact gatgtatctg cgttcaaaaa
     1741 cggtaaggtg ttaatgtatg attccatacc agttggagga gaccatataa caaatgatat
     1801 ttcaatagga ttgcatattt catattctga agcagagaaa attaaaaaac aatatgaact
     1861 tgcattaact acattgataa agaatgacca ggaaatttcc gtatatgatg taaatgatga
     1921 aataaccaga aacattaaag tttcacaggt gatttaaata attgaagcaa gggtttttga
     1981 aatactgtct ttaagtaaaa gtttgatttg caaatatgaa gacctggggg aatttgattc
     2041 aggtattgta ttaacaggtg gcggtatatc ttatgttaat ggaaacaggc aaatagctgg
     2101 tgaagtattt ggattacctg taagagtagc ttcttataga aactatgcag gtatatcaaa
     2161 acctgaattt gctgcagcag caggtattat caagtatgtt tcatcaagaa acaaaagagc
     2221 tagtattgtg tataatgtaa aagataagca agtggaaact gagcacaaaa aggatggtat
     2281 ttttaaccgt attataaaag tgctcgaaag atggtttttt tgagtaggtg aaatgaattt
     2341 tcttgaaatg tactattgca cttaagcaaa taataatata ttatttaatt attaagtaaa
     2401 tttaaaaaga atagtatcat tttgttacat cactataaca aattgaagtt agcataatta
     2461 tgggtcatat tacacgatta ttatgataaa ctaatgataa ctttaaggtt agcggatttg
     2521 caaaggtaaa agtataaggg ggaagaatac gttgctggaa tttgatgttg atatggaaca
     2581 gtttgctcag ataaaagtta taggtatagg cggtggaggg aataatgctg taaacagaat
     2641 gataaatgcc ggacttcggg gcgtagaatt tatttctata aatacggaca agcaggcatt
     2701 attcttatcg aaagccaata cgaagataca aataggggaa aagctcacaa aggggttagg
     2761 tgccggggca aatcctgaca taggcgaaaa agctgctaat gagagcaagg atgaaatcag
     2821 ccaggctata aagggagctg acatggtgtt tattacagca ggtatgggtg gaggtacagg
     2881 tacaggtgcg gcacctatag ttgcccaggt tgccaaagaa atgggaatac ttacagtagg
     2941 agtggtaaca aagcccttta tgtttgaagg aaggaaaagg atgcagtttg cagaaagagg
     3001 aattgaaaat cttaaagcat ctgtagatac tcttgttacc atacctaacg acagactgct
     3061 gcaggttgcc gaaaaaaaga cttcaattgt tgatgctttt agaatggctg atgatatcct
     3121 tagacaaggt gttcagggca tttcagattt gataatagtg ccaggcctta taaatcttga
     3181 ttttgcggat gttaaaacaa taatgttgaa tacaggtctt gctcatatgg gtattggaag
     3241 agcttcgggg gatggaagag cagaagaagc aactaagaaa gcaattgaga gtcctctgct
     3301 tgagacatct attgaaggag caaagggtgt gctgcttaat attaccggtg gtgcagacct
     3361 tggattattt gaagtggatg tagcagccgg actgattgaa aaatctgctg atcctgatgc
     3421 gaacataatt attggtgctg ttattgatga aaacctggaa gatgaaatac tcataacagt
     3481 tatagccact ggctttgagc aaggacctat tataagaaaa caggaaaaaa ccacaacacc
     3541 tgagaagccg tccaaacctt cttatgcaga gaaagctccg cctgctatag atattaataa
     3601 tgatgagctt gacataccta cttttctaag aagaaacaga tataaataac aatttactat
     3661 gaaaacacca tatataacat atatggtgtt ttttttgctt gaaaatactt gattctaccg
     3721 caaaaactca ttttttgcag caaaatcaag acttatattg tcataataaa atacaaaatc
     3781 aacaagaaat tgtgacaaaa tgctttaaaa gataagtata taataataaa ggtaatagag
     3841 aagcaacatg aagcgaaatt ggaagtttga gaaattatga tgtagcgttt gttataaata
     3901 catagtttac tgaatatatg taatattagg gattgtctat ccgctgaaat aagaatggat
     3961 tttgtattga ttgcgaggta tgaggtgtgg aaatatatct ggatgttctt atattggaaa
     4021 atgttgtaat gaattatctg attttatgga caacaggtaa aatattaaaa acacgaacat
     4081 ccaatattcg tctatttatg ggggctctaa ttggtgcagc ctatgcagca tttcttgtaa
     4141 taaaaccggg acttggaaca tacttttctg caactgccac agttattcta tctatagcaa
     4201 tggtagcagt tacattttca cctgaaaaaa taactgcatt ttttaaaact cttgtagttt
     4261 tttatgtatc tacattcgta tttgcaggtg ctacttttgc tttcttttat atgaacaata
     4321 agggtacaat tgtaagaaat gggttattat atgtattttg ggaatcaaag tggacgactg
     4381 tggtgctttc cataataact tcaattatta ttttaaatat atttcttgaa gttatacagc
     4441 agaaatattt taaggaaaaa ctaattacac gtctgaaggt aatatttgat agcaaagaaa
     4501 tagacatgtc agcattggtg gatacgggaa actcattgca ggatcctcta accaacatgc
     4561 ctgttattgt tgttgagttt acggcaatta aggaaatact tccttcagaa atatgcaata
     4621 tattcgaagg aatgcatgag tatgacttgt caagtttaag cagcaggcta tatgactcta
     4681 agtggtttaa caggtttagg ctaatacctt ttaattcact tgggaaggaa aatggtatgc
     4741 ttattggatt taaaccggat tatgttgtaa ttggcgaaca tactgataaa aaaggggtta
     4801 gcgatgtgat aattgcaata tataataaag ttctttcaaa aggcaaggat tacaaggccc
     4861 tcctgggacc tgacctttta taacatgcat agaaataatg atcaagaagg agacttaaat
     4921 catgattaaa ttcaaacatt acaaactgat attatatttg aaatatataa aggttctgga
     4981 gagatttggc ttatacaatg ggcgccatat acactacatt ggaggaagtg aggctttacc
     5041 tcctcctcta tccgatgatg aggaaagctg tttactggac cagctgggaa aaagtgatta
     5101 cggagtaaag aaaatcttaa ttgaaagaaa tttaagattg gtggtatata tagcgagaaa
     5161 gtttgaaaat acaggtgttg gtgtagagga tctagtatcc ataggcacta tagggttaat
     5221 aaaggctata aatactttta aaccttctaa aaatataaaa ttggcaacct atgcttcaag
     5281 gtgcatagaa aacgaaatat taatgtactt aagaaggaat aataagacaa ggacagagat
     5341 atcaattgat gaacctttaa acatagattg ggatggtaat gaattactcc tgtcagatat
     5401 tctgggtact gacaatgata ttatacatag gagccttgaa gacgaaattg acaaagaatt
     5461 gcttaatgca gcgcttatga agttgtcttc aagggaaaaa aagattatgg agttgagatt
     5521 tggacttgtt aacggagtag aaaagacaca aaaagaagtg gcagatatgc tcggcatatc
     5581 tcaatcatac atatcaaggc ttgaaaaaag aataattaac agactaagga aggaaataaa
     5641 caggatggtt tgaaaaacat attccgggtt gccaggttgt aaacatagaa tcggcttaaa
     5701 tagctattga taaaaaaact atgatttgtc aagggtaatt agcaaaattt tgtttaatgt
     5761 ttaagctgca agttcagtat aaaaatgacc tctatggcaa taatgatact gacagaattc
     5821 attttgcctg gagggtttta aatgcatatc aacaaagttg aaatttgtgg agtaaacaca
     5881 tcaaaattac cggtattaac aaatgagcaa aaaaaagtac tatttgaaag aatgcataaa
     5941 ggtgatgatt cggcacggga agaatttata cagggtaact taaggctggt attaagtgtt
     6001 attcaaaggt ttaataatag aggggaatat gtagatgacc tctttcaggt tggatgtata
     6061 ggcttaataa aggctataga taatttcgat gtatcacata atgttaaatt ttccacctat
     6121 gcggttccaa tgataatagg ggagatccga agatatttga gagacaataa ttcaataagg
     6181 gtaagccgct ctttaagaga cgttgcttat aaagctttac aggtaaaaga aaagcttaca
     6241 aacaaaaatt caaaggaacc tactattgat gaaatagcaa aagagttaga ggttcaaaag
     6301 gaagacgtag ttctggctct tgacgccata caggacccca tatcattgtt tgaagctgtt
     6361 taccatgatg gcggagatgc aatatttgta atggatcaag taagcgatga aaaaaatact
     6421 gatgaaaact ggatagaaga gatatcctta aaagaagcaa tgcaaaaatt aagtgatagg
     6481 gagaagctga tattaaactt gagatttttt gaaggacgta ctcagatgga ggttgcagaa
     6541 gaaatcggaa tatcccaggc tcaagtatca agacttgaga aaacagctct tcttcatatg
     6601 aaaaaatata tatgatggca ggaaaaagat aatatatcta attatagaca atggttataa
     6661 tatggtataa aatagtattg catgcaaacc tgatactatg atgggaaatg cagtatagtt
     6721 atgtgaggtg ataaagggtg aaccgtacta atgacctcaa gcagaaggaa gttatcaaca
     6781 tatctgacgg gagaaggctt ggatttgtaa atgatgtaga aattgatctg gaatgcggaa
     6841 aaattgaagc aatcgtaatt cccgggggtg gaaggttatt tggtttaatt ggcaaagata
     6901 atgagtttgt aattccttgg gaaaggattc gaaaaattgg tgaggatatt atactggttg
     6961 acctggatga caggtttatt aaaaagtatt ttacctgaaa gtttgaaatg ggggggaata
     7021 tatgaaatgt ccatattgca gttatgaaga agataaggta atagactcca gaccaactga
     7081 tgaaggtact gcaataagaa gaagaagaga atgtttaaag tgtcagaggc gttttacaac
     7141 atatgaaaaa attgaaacca taccgattat ggttataaaa aaagatagga gccgtcagcc
     7201 gttcagccgg gaaaagatat taaacggttt aatgagagct tcggaaaaaa ggcctgtttc
     7261 tgtggcagac ctggaaaata tagttaatga aattgagagt catatataca attctacaca
     7321 aaaagaaata acatctaagg aaattggaga aatggtaatg ggccgactca aaaatcttga
     7381 tgaagtagcc tatgtccgtt ttgcttcggt gtacaggcag tttacagatc taaacaattt
     7441 tatgaacgaa ttgaggaaaa tacttgagga aaagaaataa agttatcaaa ttaacaattt
     7501 gttaacatat tattatttta ttgtgttatt atagaaatat attagcttat ccttctttaa
     7561 acttgtgaac ttgttgtccc ctccttttgt aattatgaaa taaatatata taggaagtga
     7621 agcagcgatg tttggtgtta cagaacaaaa aatagaaaaa tggaagtcta aaaataaagt
     7681 tgataaaatt gttgaagcaa tgaaccataa ggataaggta ataaggttgg cagcgataaa
     7741 agctggagct ggcatgaaag atgagaaaat ttttaatgca ttagtaatat ttctgaatga
     7801 tcccgatccc ttaataaggg caggttcagt tgattcactt ggctccttgg gaattgcgag
     7861 agcccaggat catttgcgat ttgtatcact gaatgatagt gacgaaaatg ttagggaaaa
     7921 ggcattagaa gcaatgaaga acataatagc aaaaaaaatg taggctgaat aatttagtgt
     7981 attaaaaact agtacactgg tacatgttaa aggagggggc aatagtttat tttgaataat
     8041 catacatatg tttctgattt ttttatatca tgtctttagt ttttaacttc tgtagtaaaa
     8101 ttctaacatc tattttattt atattatata ataatactat cttatcttaa actcatttaa
     8161 ccaactatca taaaatatgt aatttaatac aaatattgtt gaaataaaca taataatagg
     8221 atataatgca ataaaaaggt aaatgtttac tgaatattaa ttttatatca gcaatatgaa
     8281 aggaattact ttatgctttc taaaataaaa agctgtggtt taatgggcat cgatgggttt
     8341 attataaatg ttgaaacaga tattagcaat ggtataccgt cttttgaaat agtaggtctt
     8401 ggagatattg cagttaaaga agcaaaagaa agggtaagga cagccataaa gaatgccagg
     8461 tatgaatttc ctgttaagag aattactgtt aacctggctc ctgcaaattt aaggaaagaa
     8521 ggttctgcgt tggatttgcc cattgctatg ggtatattgg cctgcacagg tcaaatagac
     8581 tcttctcttg tcaaggattg catatttatt ggagaacttt ctcttgatgg agatataaaa
     8641 ggggttaatg gtgtactttc tatggcttta tgcgcgtatc acgagggtat tggaaatata
     8701 attctccctg ctgataatgc ggatgaggct gctgtagtag aagggttaaa tgtatttccg
     8761 gcaaaaaata tatcagatat tgtattacat cttaacggac atttaagaat taaaaaacac
     8821 gtaatagaaa ttcgcaatat tttctgtaat gatagtgata ataatagtga taatgacaat
     8881 gataatgaaa tttccgggga tgatttctgt gaagtgtgcg gtcaggaaaa tgtaaaaaga
     8941 gttctcgaaa tcgccgctgc gggattccac aacttaatga tggtgggccc gccgggttct
     9001 ggaaaaacaa tgcttgcaag aagacttcct tctatacttc caccattaac ttttgatgaa
     9061 gcactggaag taacaagaat atacagcatc gcgggaatac taccgacgaa aacaccattg
     9121 ataactgcca ggcctttccg gagtccacac catacaattt caactgccgg ccttgttgga
     9181 ggaggaaaat accctaagcc cggagaaata agtctttctc attatggggt gctttttctt
     9241 gatgaaatac ctgagtttga aaagcatacg ctggaagtat tacgccagcc tttggaagat
     9301 gaaattgtta caatatcaag gcttagcgca agcatcacat atcctgccaa aactacgctg
     9361 attctcgcag gtaacccatg cccttgcggt taccttacag atgaaacaaa gaagtgcaca
     9421 tgtacatata atcaaataca gaattacatg ggtaaactta gtggtccgtt aatggacaga
     9481 gtagacctgt atgtagaagc tgctcctgtt aagtataaag atctggattc aaatccatgt
     9541 ggggaaaaat caaaggatat caggcaaaga gtgataaaag cacgaaatat tcaactagaa
     9601 agatataaaa acaataaaat attttcaaat tctcaactta acactggatt aattaaaaaa
     9661 tactgtagac tggacaacca atgcagctta ataatgaaaa atgcatttga aaagttcggt
     9721 ctgagtgcca gagcatataa cagaatatta aaggttgcca gaactatcgc agatcttgat
     9781 gaaagcgaaa atgtcagggc ttatcatctt gcggaagcta tacagtacag atttaccaaa
     9841 gcatggaagt gagaggtggg aagttagaag tgggaaatca gaggtgggga gttaaatatt
     9901 gattccacta cagaaagtta tgtactaaca aaatttcgat agcatccata agatgcagcg
     9961 agcggaaatg tcttacctct tacctctaac tcacccctaa cttctaaggg cgcattttct
    10021 tcttatgata caaaatatgg cagcatacga ttttccgagt ataaaa
//
LOCUS       DAOSDD010000166        10037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2543_length_10037_cov_6.070327, whole genome shotgun sequence.
ACCESSION   DAOSDD010000166 DAOSDD010000000
VERSION     DAOSDD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_10037_cov_6.070327"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /gene="metG"
                     /locus_tag="PK733_13575"
     CDS             complement(<1..1501)
                     /gene="metG"
                     /locus_tag="PK733_13575"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ11606.1"
                     /translation="MNILIGNAWPYANGSLHIGHIAALLPGDVLARYFRAKGDTVFFV
                     TGSDCHGTPVAIRARQENKTSKEISDYYHNEFANCFNRLGFSYDLYGKTSSNEHKSFV
                     TDFHRKLYNGGYVYEKSTPQAYCDKCKQFLPDRFVKGLCPHCSEFARGDQCEACGSVL
                     EPENLLQAECTICGEPPEFRSTNHLYLAVTRMKNELERYIESHSDWRKNAYEMSKRYI
                     HEGLRDRSLTRDLDWGIDVPRDKFDGKKIYIWAENVLGYLSDCYTLCNQQGLDFYEFW
                     NNCRQYYVHGKDNIPFHTIILPSLLLGRTDVKLKLPDDIISSEYLTLEGRKISTSRNW
                     AIWAKDIIERYNPDSIRYFLIGNGPEKRDTDFTWREFINSHNGELLGAYGNFVNRSLV
                     FIQKYFENKVPEAQCNQNILQLLTKLYPNIGGLIEQGNFKEALDTIFVFVRAANKYFD
                     EEQPWITIKNSVEKCKETLNTCVQIIANLSYLLGPFLPFSSAKIRNILKL"
     regulatory      2234..2320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4128..4487
                     /locus_tag="PK733_13580"
     CDS             4128..4487
                     /locus_tag="PK733_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOJ11607.1"
                     /translation="MHKDDFWSAVDTLIAQSEIVIDRPKGTKHPRFDFIFPLDYGYLK
                     DTTSPDGGGIDVWRGSFSEPVCDAVICTIDLLKKDSEIKILIGCTEEEKAVIMSFHNN
                     SECMKGAMIRRESEAEI"
     gene            4484..4867
                     /locus_tag="PK733_13585"
     CDS             4484..4867
                     /locus_tag="PK733_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11608.1"
                     /translation="MITYRKAKPEDIYPAFELALRVFIEFEAPDYEQDAVGKFKSDCI
                     DKEGMLFPRTAHKGNNGHTWFQDVWIFKHDCPIEAVRFQEGETCDAMWVTADKICGMM
                     AAGEFLSERFYPYFDEMVDKWRVKL"
     gene            4864..6453
                     /locus_tag="PK733_13590"
     CDS             4864..6453
                     /locus_tag="PK733_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HOJ11609.1"
                     /translation="MTLNEAIQIMTNEIVTVLADNKPTIYLFGSVALDDFQLGWSDID
                     ILVLTNCEITEQQAETLVGLRQALLERYLGNPYFRLFEGGMLSADAFLQDKKERTVYW
                     GTSGQRITDSYKMDSFGMAELLDSGMLLYGEDIRSQLTYPAYSQMRDDIARHVQAARK
                     YGNPVGWLLDIARGIYTLRTGKIVSKTATGEWALDEGLCPDADILQKAVQIRKEPLHY
                     SKEDKSVDNAAIRRFADVLEDEVANTVQYMAESELQRMGISFTSLSLIRNKDGVSVWR
                     VTTADNSYVIKCFDKPEYRREISNYQLLKSLSVPTLRVIAQTDCSLLLEDIERSDYRL
                     GTADDMSDPKIARLIAEWYKILHQKGRDYLDTHDFIDEYDKLTIENLKLVQERTGTSD
                     AYVWQVIKDNFEQIRSAVMSLPHTLVYSDFHYSNLAVARDGLSALVFDYNFFYKSYVY
                     FDIRNVCWDFSEEAKVAFLSEYGEYDEREVVVDYVADALSGLVIASQREVFPNWAKDS
                     VEGIKNGQILTAVERLLEVIA"
     gene            6450..7241
                     /locus_tag="PK733_13595"
     CDS             6450..7241
                     /locus_tag="PK733_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ11610.1"
                     /translation="MRNAEILRIAMGQHAIDANCSPDDFTRTENVVVISKPNENARRY
                     LNLPFFCDLITYGSNIVASVDEQIYDFVKKYIDTKYPHGCFEMPQIHHLTNEFIKYGF
                     LPCYQAEYWLPDVDVLYELPCRYDTRVLEPNDFTDLYLPQWSNALSPARPHLDMLGVG
                     AYDDSKLIGLSGCSADCDTMWQIGIDVLPEYRRQGVAAALTSRLAIEILKREKVPFYC
                     CAWSNLGSVRNAIKSGLRPAWVEHTAIEKEKALEWNENMHFSKNV"
     gene            7269..8159
                     /locus_tag="PK733_13600"
     CDS             7269..8159
                     /locus_tag="PK733_13600"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ11611.1"
                     /translation="MLKTVHLSHSIPLIPHTPRLGAFHSGDYARKMERSPFTIEEGMR
                     DMQKTAEEYSDEVAAYPGDICLFGHNHLQYLGSVAGKILLNPGSCGMPSDFDIRVPYA
                     LMNDTGDIAEIQLHRVEYDVEQTIKAILEFNGFAHAQFWGKLRAAILKTGSDIPMNRF
                     WQHAREIGGGVFPMQNEIWHKTITSFEFDYNWSIDDRRRFGERHDVIHHYDALIDENN
                     DPVHDTPSGRAYMDKWDGQAFIDEMQLSMDKDVLEVGVGTGRLAVKVAPNCRKFCGID
                     ISTKTVERAKENLHANGNAS"
     gene            8143..8316
                     /locus_tag="PK733_13605"
     CDS             8143..8316
                     /locus_tag="PK733_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11612.1"
                     /translation="MAMPADKNQSEFIDMGNRKIKIYPDNPADICGYIEAVLPAFTES
                     EQYTISNGGINYL"
     gene            8313..9182
                     /locus_tag="PK733_13610"
     CDS             8313..9182
                     /locus_tag="PK733_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ11613.1"
                     /translation="MIYLVRQGQTDWNLFKKFNVRQGETDWNLFKKFNGCTDAELNQT
                     GIAQAKLQAENLRSVSFDACFCSPQKRALQTCEIIYKGQIVFDDRLTEIKCGEFEGTA
                     ETSDSMKLFWQAIKSGDKGTESFMEFMKRSCDFCDMIAEDYIGKNVLIITHAANIRVI
                     DYYFSGKPKDYDFNKPTAIGMSPKERWDSSLENGIKLRFFSPVELEEIFLHAQQRRVN
                     KYGVVSFEGNTYEAPVELIGMNVIMRYNPFHLEYLHVYYQDKYFGTAKIIDLKTQRHK
                     SVGAVPEESGYVV"
     gene            complement(9193..9615)
                     /locus_tag="PK733_13615"
     CDS             complement(9193..9615)
                     /locus_tag="PK733_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11614.1"
                     /translation="MKHFATDDGRILTPPKELEPLLKGSFPAFSKLLGRIRFFYMVDE
                     IWDGKSSLIFSADDEQLIAATLGDGVFYVHIANKDFRIADETSLDNVFKALEKIVPIG
                     SQRPFEQLTANPDPNVFPCGYRCDLCLGSKEYDENNRN"
     gene            complement(9671..9880)
                     /locus_tag="PK733_13620"
     CDS             complement(9671..9880)
                     /locus_tag="PK733_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11615.1"
                     /translation="MLFCNTCVRNDEDKCYDCRYDIACENLSDKKVADEFEIVRLKPC
                     EYAVFTCEFDSEMTLKDAHEKPDAE"
BASE COUNT         2966 a         1920 c         2201 g         2950 t
ORIGIN      
        1 caagctttaa tatgtttctt atttttgcag atgaaaacgg caaaaagggg ccaagtaggt
       61 aagaaagatt ggcaataatc tgcacacagg tattcagtgt ttctttgcac ttttcaacac
      121 tattctttat tgtaatccac ggttgttcct catcgaagta tttatttgct gctcgtacaa
      181 atacaaagat tgtatccagt gcttccttga agtttccctg ttctataagt cccccgatgt
      241 ttggatagag ctttgttaac aattgcaaaa tattttggtt gcattgcgct tccggcacct
      301 tgttttcaaa atacttttga ataaacacaa ggctgcggtt cacaaagtta ccataagctc
      361 ctaaaagttc gccgttatgg ctgttgatga attccctcca tgtaaagtca gtatctcttt
      421 tttcgggacc gttgccaatc agaaaatagc gtatggaatc aggattatag cgctcaatga
      481 tatcctttgc ccatatcgcc cagtttctgc tggtggaaat tttcctgcct tcaagggtca
      541 gatattcgct ggatataata tcatcgggca gcttcaactt gacatcggta cggcctaata
      601 gcagcgaagg aagaataatt gtatgaaaag gtatgttgtc cttgccatgc acataatatt
      661 gcctgcagtt gttccagaat tcataaaaat ccaatccttg ctgattgcat agcgtataac
      721 agtctgaaag gtagcccaga acattttctg cccagatata tattttttta ccgtcaaatt
      781 tatccctcgg gacatcaata ccccaatcaa gatcccgggt aaggcttctg tcgcgaagtc
      841 cttcatgtat atacctcttt gacatctcat aagcattttt tctccagtca ctgtggctct
      901 caatatatct ttcaagctca ttcttcatac gggtgacagc caggtaaaga tggttggtag
      961 accggaactc aggtggttct ccgcatatag tgcattctgc ctgaagcagg ttttccggct
     1021 ccagtacact tccgcacgct tcgcactggt cgccccttgc gaactcacta caatgaggac
     1081 acaatccttt tacaaagcga tcgggtaaaa attgcttgca tttgtcacaa tacgcctgcg
     1141 gagtgctttt ctcataaaca tacccaccat tgtagagttt cctatgaaaa tctgtaacaa
     1201 agcttttatg ctcgttactg gatgtcttcc catataggtc ataagagaag ccaagacgat
     1261 taaaacagtt agcaaactcg ttgtgatagt aatcactgat ttccttagat gttttgtttt
     1321 cctgtctggc tctgattgcg accggtgtac catgacaatc actgccagtg acaaaaaata
     1381 ctgtatcacc ctttgctctg aagtaccttg ccagtacatc tcctggtaat aatgcggcta
     1441 tatggccgat atgcagagag ccgtttgcat atggccacgc atttccaatt aaaatattca
     1501 taaatgcacc tcatttctaa ttgatttgga ttaacatatt tatttaagca aaatgcacat
     1561 taaaaaagct ctcacctcca ggataaatat cctggggacg agagcatgat acttcgtgtt
     1621 accaccccgg tttattgatg cttcacagca ccaacctcaa caagtacggc aggataaatg
     1681 attcctgatt atactccggc actataacgg gagcacccgt cgcagtctaa accctaaaaa
     1741 ggattcggtg cgaaagctct gagaccatct tcggttatct attctttact ccttctcagc
     1801 ttaccggctg gtcgcaaatt gcatttgctg ctcgcccatg cggcttggga gttctctgta
     1861 aaagatatca acaacttact cttctcttca tcgcgtcttt aatttttatc ttaatcgcag
     1921 ttaggccata tttagtcttt aatatgatat ggcattaaat ctggcttcat tcataactta
     1981 aactgaatgt aattttctgc aagttatata tatcttaata cgcaatgtac ttacagtcaa
     2041 tagtctattt gactacattg actatattca ataaaattaa ccagttaact gaattataca
     2101 agaaagtgaa tgcttattct gatagttcaa atttaggcac agacattttg aactgttaaa
     2161 gaaaataagc aagctttgac aaatttcaac aatgctattg gtgtataata gaattataaa
     2221 taaaatttga aaaaaacgat aaaggcaaac ttgttgaaag acaaggacgc aaagactcgg
     2281 gtctaaagcg gttttccgct acgatggccg ggtcgccgga actaagctgc tttaggctca
     2341 ttggtgcttt ataacatcag tgggcctttt tgtatacaac ctgtattact tgatacaacg
     2401 acttcgagcc actgactttg atctgccgaa aagctgagag aaataaaacc tgatatagca
     2461 atagtcttta taactgcaca tcagaatact ctctcgatgc attcatagat cgttatgatg
     2521 tttaattaca gttgagagag cctgaagact tctctggggt ggaatttttg atctatattc
     2581 cttggaataa ataattgctt tagttataga aaaattatct tgttctgacg ttatttaaat
     2641 atgaatgaag gaaggtgctt gagattaaat ctgtgcttaa ctactgattg aaattgattg
     2701 tagcattaac tatggtaaaa aagtataaca ctgtagtcat ttgaatttta caacatactt
     2761 acaggattac ggtgagtaat atgcacttta tattttctgg caactggctt tttggagaaa
     2821 tgctgcaccg tatgcctata tgggcgctcc cataatactt tagtctgaat cagtctaaat
     2881 aaaagttatc ctttcgaata ggtttgacta gttggaaaat tatgatatta ttgatatagt
     2941 agaaaagttt ggtaatgtga taatgattaa cttagtcata aggggattat tttgtgaata
     3001 acattgtagt catatttaaa aggaagtatt gaagcagttg gggtttacca gaaagtcttt
     3061 gatgctacac taggttacca cgaactaaat tctgataata cataaagtaa gctgagataa
     3121 atttggatga gcagaataat ttcaataaat aagtcaaatg attagggaat tttaggtaga
     3181 aatatgcaat ttggtattat cttcggtagt ggagatgagg aatcgttgaa gaaatcatac
     3241 taagttttaa agagaatggg aaagtcctct atacgttagg ttcatgtgat tgaggtagat
     3301 taaaatccta tatccaatgt gatttttgtg gatatagaac gtctgacgga ttatggattt
     3361 tcggaaaagt ttatcacgct gataaagacg cttttaccga acagggcagt tatttgggtg
     3421 ataagtctaa tatcggcttg ggagtttagg tgaagattat ggacagatac atagctcatg
     3481 aattgattat aaaagccaat gagctattga aaaacagcag ctttgaatat gccctttgcg
     3541 gcgggttagc gattgagtta tttttaaatc gaacaatccg caaacatggc gatattgacg
     3601 tatctgctta ttggcatgac agagataaga ttattctcta catgcaatca ttgggttgga
     3661 atgtatttga aatgtgcggc ggcggaatag ctcatcacat taccgatgtt aaatttcaga
     3721 taaaggcaaa gcgaaatatc ttttgcttca aagacggatg cagtctggtg aagctctcgc
     3781 ctcatggcga aacagatatg tattatcttg attttgacca tagcggacaa acaaaacttg
     3841 attttatcga gtttttattt aacaaccgta atgctgattc ttttctatat gctcgaaacg
     3901 agaacatttc actaccgata acaaaagcga tattgactcg gaacggtatt ccctatcttg
     3961 cccctgaact ggttttactg tataaatcta ctgatacgga ccgcgaaggc tatcagttag
     4021 actatgattc cgcaaaggct gaaatgagtg ctgagcaaag aggttggttg cagactgcgc
     4081 tgaaaacagt gagtccgtta gggcataaat ggctggagaa aaatgtactg cacaaagacg
     4141 acttttggtc agccgttgat acgctgattg cccaatcgga aatcgtcatc gaccgcccga
     4201 aaggaacaaa gcacccaagg tttgatttca tctttccttt agattatggc tatttgaaag
     4261 ataccacttc acccgatggc ggtggcattg acgtgtggcg tggaagcttt tctgaaccag
     4321 tgtgtgatgc tgtcatttgt acgattgact tgctcaaaaa ggattctgaa atcaagatat
     4381 tgattggctg tactgaggaa gaaaaggctg ttattatgag ttttcataat aattccgaat
     4441 gtatgaaggg tgcgatgatt cggagagaaa gcgaggctga aatatgatta cttacagaaa
     4501 agcaaaaccg gaagatattt accctgcttt tgagcttgcg ctacgagttt ttatagaatt
     4561 tgaagcacca gactatgagc aggatgctgt cgggaaattc aagtctgatt gcattgacaa
     4621 ggaagggatg ctgttccctc gtactgccca taaaggaaac aacggtcaca catggtttca
     4681 ggacgtatgg atttttaagc atgactgtcc gattgaagct gtacgctttc aagagggcga
     4741 aacctgcgat gccatgtggg tgaccgcgga taaaatctgc ggaatgatgg cggcaggcga
     4801 attcttgagt gagcggtttt acccatactt tgatgaaatg gtggataaat ggagggtgaa
     4861 actatgacac tgaacgaagc cattcaaata atgaccaacg aaatcgtaac tgttcttgca
     4921 gataataaac cgaccattta tctattcggc tctgttgcgc ttgacgattt tcagctcggt
     4981 tggagcgata ttgacatcct tgttcttact aattgcgaga tcacagaaca gcaggcagaa
     5041 actctggttg ggcttcgtca ggctttactg gaacgttatc tcggcaaccc gtattttcgg
     5101 ctgtttgagg gcggaatgct ttcggcagat gcgttcttac aggataaaaa agaaaggacg
     5161 gtttattggg gaactagcgg ccagcgcatc accgacagct ataaaatgga tagcttcggc
     5221 atggcagaac tgcttgacag cggtatgttg ttgtatgggg aagatattcg tagccaattg
     5281 acttaccctg catactccca aatgcgtgat gatatcgctc gtcatgttca ggcagcgcga
     5341 aaatatggaa atccggtggg ctggctcttg gatattgcgc gtgggattta taccctgcgc
     5401 acaggtaaaa ttgtttccaa aaccgcaacc ggtgaatggg ctttggatga aggcctttgc
     5461 cccgatgcgg atatattgca aaaagccgtg caaattagga aagaaccact tcactattca
     5521 aaagaagaca aaagcgtcga taatgctgca attaggcggt ttgcggatgt gttggaggac
     5581 gaagtggcaa acactgtgca atacatggcg gaatccgaat tgcaacgcat gggcatatcc
     5641 tttacctccc tctctttaat ccgcaataaa gacggcgttt cagtttggcg agtaacaaca
     5701 gccgacaata gctatgtaat aaaatgtttt gacaagccgg aataccgccg tgagatttcc
     5761 aactatcagc ttctaaaatc gcttagtgtt cccacgttga gagtaatcgc tcaaactgac
     5821 tgttctcttt tgcttgagga tattgagcga agcgattaca gattaggcac agcagatgat
     5881 atgagcgacc cgaaaattgc aagacttatt gcggagtggt ataaaatcct ccaccaaaaa
     5941 gggcgggatt atttggacac gcacgatttt atagatgaat acgataaact gacgattgaa
     6001 aacttaaaac ttgttcaaga aaggacaggg acaagcgatg cgtatgtttg gcaagttatt
     6061 aaagataatt ttgaacaaat ccgttctgcc gtgatgagcc ttccgcacac acttgtctat
     6121 tctgactttc attattccaa ccttgccgtg gcgcgtgatg gtttgtcggc attggttttt
     6181 gattacaatt tcttttacaa atcatatgtg tattttgaca ttcgcaatgt ttgctgggat
     6241 tttagcgaag aagcaaaagt cgctttcctg tctgaatatg gcgaatatga cgaacgtgag
     6301 gttgttgttg actatgtggc agatgcgttg tctggattgg ttatcgcttc tcagcgagag
     6361 gtttttccga attgggcgaa ggattcggtg gaaggaataa aaaatggcca aatactaaca
     6421 gctgtagaaa ggttattgga ggtaatagct tgaggaatgc tgaaatctta cgtattgcaa
     6481 tgggacagca cgctatagac gcgaattgtt ctcctgatga ttttacaagg acagaaaatg
     6541 tggtagtcat ttcaaagcca aacgaaaatg cccggcgtta tctaaatctg ccgtttttct
     6601 gcgacttaat tacatatggc tccaatatcg ttgcttctgt tgacgaacag atttatgatt
     6661 tcgttaaaaa gtatattgac acaaaatatc ctcatggctg ttttgaaatg ccgcagatac
     6721 atcatctgac aaacgaattt attaaatacg gctttttgcc gtgctatcaa gcggaatatt
     6781 ggttgccgga tgttgatgta ttgtatgagc ttccgtgccg atatgacaca agggttttag
     6841 agccgaatga ttttaccgac ttatatctgc cgcaatggag caatgcgcta tccccggcaa
     6901 gaccgcattt agatatgctt ggggttggtg catatgatga cagtaaattg attggccttt
     6961 ccggatgctc cgccgattgt gatactatgt ggcaaatagg aattgatgtt ctgccggagt
     7021 accgccgaca aggtgttgca gctgctttga cttcccggtt agcgattgaa attcttaaaa
     7081 gagaaaaagt cccgttttac tgttgtgcgt ggtcaaatct tggttcggta cgcaacgcta
     7141 ttaagagcgg attacgtccg gcatgggttg agcatacggc aatcgaaaag gaaaaagcat
     7201 tggaatggaa cgaaaatatg cacttttcga agaatgttta aagagcattc tgatctggag
     7261 ggtacggcat gttaaaaacc gttcatttat cccacagtat tcccctgatt ccccacactc
     7321 ccagacttgg cgcatttcat tcaggtgatt acgcaagaaa aatggagcgc tcccctttca
     7381 caattgaaga aggaatgcgt gatatgcaaa aaaccgccga agaatattcg gatgaagttg
     7441 ccgcctaccc tggcgatatt tgcctttttg gacataatca tttgcaatat ttgggcagcg
     7501 ttgcagggaa aatattgctt aacccgggaa gctgcgggat gccctctgat tttgatattc
     7561 gcgtgcccta tgcgttgatg aatgacacag gagatattgc ggaaattcag cttcaccgcg
     7621 tcgaatatga tgtggagcaa actattaaag ctatccttga atttaacggt ttcgcccatg
     7681 ctcaattttg gggtaagttg cgtgcagcta tacttaaaac gggatcagat atcccaatga
     7741 accgattttg gcaacacgca agagagatcg gtggcggcgt atttccgatg caaaatgaaa
     7801 tatggcataa gacgattaca agctttgaat ttgactataa ctggagcatt gatgatcggc
     7861 gaagatttgg agaaaggcat gatgtaattc accactatga tgcactaatt gacgagaaca
     7921 acgatcctgt tcatgacact ccatcaggga gagcttatat ggacaaatgg gatggacagg
     7981 cgtttattga tgaaatgcag ttatcaatgg ataaggatgt gcttgaagtt ggtgtcggca
     8041 caggtagatt ggctgtaaaa gttgcaccaa actgccgtaa gttttgtggc atcgatattt
     8101 cgacgaaaac cgtggagcgt gcgaaagaga atctgcacgc gaatggcaat gccagctgat
     8161 aaaaatcaaa gtgaatttat cgatatgggc aatcggaaaa taaaaatata ccccgacaat
     8221 cccgctgata tctgtgggta tattgaagct gtactgcctg ctttcactga atcagagcaa
     8281 tatactatat caaatggagg aataaattat ttgtgatata cctcgtccgt cagggtcaaa
     8341 ccgattggaa tttattcaag aagttcaatg tccgtcaggg tgaaactgat tggaacttat
     8401 tcaagaagtt caatggttgt acagacgctg agctgaatca aacaggaata gctcaggcaa
     8461 aactgcaagc cgaaaacttg aggagtgtta gttttgatgc ttgtttttgc agtcctcaaa
     8521 aacgagcact tcaaacctgt gaaattatct acaaaggaca gattgtattc gatgataggc
     8581 tcaccgaaat aaaatgcggt gagtttgagg gcactgctga aacttcggat tctatgaaat
     8641 tgttttggca agcaatcaaa agcggtgaca aaggcacaga aagtttcatg gaatttatga
     8701 aacgtagctg cgatttttgc gatatgattg cggaagacta tataggcaaa aacgtcttaa
     8761 tcattaccca cgcggcgaac atacgggtaa tagactatta tttcagcggc aagcccaaag
     8821 actacgattt taataaacca actgctattg gtatgtcacc gaaagaacgt tgggattcat
     8881 cacttgaaaa tggcataaag ctaaggtttt tctcccctgt tgaactggag gaaatatttc
     8941 tgcatgctca acaaagaaga gtcaacaaat atggggtagt ttcttttgaa ggaaacacct
     9001 acgaagctcc tgtagaactt atcggtatga atgtaataat gaggtacaat ccgttccacc
     9061 tggaatatct tcatgtatat tatcaggata agtattttgg cacagcaaag attatagact
     9121 taaaaactca aaggcataaa agcgtaggtg ctgtacctga agaatccggt tatgtagtat
     9181 gaaaattcta cattaatttc tattgttttc atcatattct ttgcttccaa ggcacaaatc
     9241 gcaccgataa ccgcagggaa aaacattcgg gtcgggatta gccgtgagtt gttcaaacgg
     9301 acgttgagag ccgataggta cgattttctc taatgccttg aaaacattgt ccaaagaggt
     9361 ttcatcagca attcggaaat ccttattagc gatatgtaca tagaaaacgc cgtcaccaag
     9421 cgtagcagca atcaactgtt cgtcgtccgc actgaaaata agcgaggatt tcccgtccca
     9481 aatttcatct accatataga aaaagcggat gcgtccaagc aattttgaaa aagctggaaa
     9541 tgagcctttt aacaacggtt ccaattcttt cggcggcgtt aaaatccttc cgtcgtctgt
     9601 tgcaaaatgt ttcatatcgc cattttcacc tttcttttcg taaaatcgga taccacattg
     9661 ttgcgatgaa ctattctgcg tccggctttt cgtgcgcatc ctttaatgtc atttcagaat
     9721 cgaactcgca ggtaaacaca gcgtactcac agggttttaa cctgacgatt tcaaattcat
     9781 ctgcgacttt tttatcgctg agattttcgc aggctatatc atacctgcaa tcatagcatt
     9841 tgtcctcatc gttgcgtaca caggtgttac aaaacagcat atatacggtt tcggaacaag
     9901 ccgccttttt cagacgctca actgaaccgt cgttaatcct ttttcggata aactcttcgt
     9961 tatttccagc aacggaagct gcgctttaag cgcatcggga tcggctgtgt agccttcctt
    10021 cggcgttttc aacgccg
//
LOCUS       DAOSDD010000167        10033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2546_length_10033_cov_5.260774, whole genome shotgun sequence.
ACCESSION   DAOSDD010000167 DAOSDD010000000
VERSION     DAOSDD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_10033_cov_5.260774"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PK733_13625"
     CDS             complement(<1..464)
                     /locus_tag="PK733_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11616.1"
                     /translation="MKKIVSLLNKELTGKIFSEETLSELSKKGELIINNDTANPDPER
                     TKELIEGANIVITSWGSPNLNEDILSAAPHLELVIHAAGSVKGIVSQELWDKGVRVTG
                     SAPVLGMGVAETTLGLTIMSLKNIWKLSESLRNGGWSEGRNNVKEIYDVTIGV"
     gene            complement(828..1520)
                     /locus_tag="PK733_13630"
     CDS             complement(828..1520)
                     /locus_tag="PK733_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ11617.1"
                     /translation="MINKWIKVLLNCRLFYGIDEEELENMLECLKPKISSYEKNEFAA
                     ISGNKYDGLGLVLSGSVAVTKENAAGDRIIIDILGPGEIFGEIAAFAGTEVWPATVIA
                     QTACTVMYITPKIILGTCRKLCISHKHLISNMLNIVSKKALLLNQKVQYLSMKSIRGK
                     ISSYLLEQYKKERQTTFVMPMKRNELADFLNITRPSLSREMCRMRDEGVIEFYRESVK
                     IMDLGALTSMAE"
     gene            1975..2694
                     /locus_tag="PK733_13635"
     CDS             1975..2694
                     /locus_tag="PK733_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ11618.1"
                     /translation="MKRKIVKIDENKCNGCGICVNACHEGALQLIDGKAKLVSESYCD
                     GLGACLPACPEEAIIIEERIAEAFDSESICGQKEENNTLACGCPGSHAKSLERKTEKL
                     QASPSQQKDMYSEIAESQLRQWPVQIKLVPVNAPYFKNAHLLVAADCTAYAYANIHND
                     FMRNKITLIGCPKLDDINYAEKLAEILKAHDIKSITVLRMEVPCCGGIVYAVKQAMLN
                     SGKMIPWNVVTISTNGDILEE"
     gene            2778..3110
                     /locus_tag="PK733_13640"
     CDS             2778..3110
                     /locus_tag="PK733_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ11619.1"
                     /translation="MIEKQYKFIKTDTKLIERVLEDDNAGINHMVLPKGDALPEHFSN
                     SHVYMIVVRGNITLRLDEQETHEYPAGSIINIPYNTKMNVSNQHDEVLEFFVVKAPSP
                     RKFASLKP"
     gene            3237..4799
                     /gene="hcp"
                     /locus_tag="PK733_13645"
                     /gene_synonym="priS"
     CDS             3237..4799
                     /gene="hcp"
                     /locus_tag="PK733_13645"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOJ11620.1"
                     /translation="MSNMFCFQCEQTAGGKACTQSGVCGKKPDVSNKQDELICALVGL
                     ARAAAGKVPGRLANELMMQGLFATITNVNFDKERINELKNQILDEKERLGGAEDLAFD
                     TLWNGDSDIVSLRSTLLLGLRGMAAYAWHAYVLGKEDPEVTSWFYKGMRAIGEEHTID
                     EWLNLLIGFGLINLKCMELLDNANTSIYGHPVPTRVTTIIEKGPFIVVTGHDLHDLKQ
                     LLDQTQGKSINIYTHGEMLPAHGYPELKKYPHLKGNFGTAWQNQQKEFDSIPAPILFT
                     TNCLMPPKASYADRVFTTAVVGYPQIEHIPDRDGSKDFTPVINKALELGGWAEDKHLT
                     GINGGTELITGFAHNTVLGVADKVIDAVKAGAIRHFFLVGGCDGAKPGRNYYTDFVKK
                     TPEDSVILTLACGKFRFNDLNLGEIGGLPRIMDMGQCNDAYSAIQVALALANAFNCGV
                     NDLPLTLVLSWYEQKAVCILLTLLALGIKNIYIGPSVPAFFSANVLNVLVEKFGIKPI
                     STPDEDLKAILG"
     gene            complement(5151..5903)
                     /locus_tag="PK733_13650"
     CDS             complement(5151..5903)
                     /locus_tag="PK733_13650"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOJ11621.1"
                     /translation="MKVKVLKNSDELGKAAAAFSAEIIRQAIEKDGKARIILSTGASQ
                     FDTLKYLVKEDINWSKVEMFHLDEYINLPETHPASFRKYLKERFVSIVNPGKVFFVDG
                     EGDVESKILALSKEVTREPIHLGLIGIGENAHIAFNDPPADFETTKPFIIVNLDDKCK
                     KQQVGEGWFPTIDDVPKQAISMSVHQIMQCNVILSSVPYKVKANAIKITLENDLTNMI
                     PATMLKTHKNANIYVDEDSVSAVDKNVLAKYL"
     gene            complement(6077..7108)
                     /gene="splB"
                     /locus_tag="PK733_13655"
     CDS             complement(6077..7108)
                     /gene="splB"
                     /locus_tag="PK733_13655"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HOJ11622.1"
                     /translation="MRLFMPERVIFEPSSLNYPLGMELFNFFENNNNVEIIEAGIKNA
                     FRYIPGNSTSSKYVHSKKTLVVTTMKGKELDVCKPSADFEFSLVSNCPGNCEYCYLQT
                     TQGNKPYIKIFVNIEDIFNIIKQHIKEDEDEITTFEAASLGDPLSLEHITGSLAKTIE
                     FFGSLEKARLRVVTKFSNVDSLLKLKHNGHTRFRISMNSRYVIDNFEHNTATLDERME
                     AALKLVDAGYPIGFIIAPIMVYDNWKEEYFELFSKLGERLGPQFSGNEITFELIQHRF
                     TSVAKKLIIERFPRTKLDMDEEHRTMKWGRFGRYKYVYPKEQAGEIKEFITNLIKERF
                     PEAVIEYFT"
     gene            complement(7280..7696)
                     /locus_tag="PK733_13660"
     CDS             complement(7280..7696)
                     /locus_tag="PK733_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ11623.1"
                     /translation="MSVFLLALFGMICWGIAPIFAKVGLKNMNPSVGLVFRTLFAASV
                     VTCWAVANGTFSQMKGIPVYTLLLIAIESLLATLVGDLAYFAAIKRGDVSLVTIIMSS
                     SPFVTLLFACIFLGEKITLVRFIGAAFVITGIILII"
     gene            8212..9219
                     /locus_tag="PK733_13665"
     CDS             8212..9219
                     /locus_tag="PK733_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ11624.1"
                     /translation="MKKVLIIILSIVLFTGSIVFAGIKSFNRKYSQHNDSRAEEQPKP
                     IPGNIAYEGGDVTEIINVPGGNSESESGSDSDSGTLNAIASGPVKEVPWENEDTFKEA
                     LMQSGATTILAAFRTVLKDPLPGEEFNVHLAARMLAGTILKPQEIFSQNKLLGPYTES
                     RGYQEGPTYMGSRLAKTYGGGVCKIASTLYNVSVLSNLQIIERHAHSMPVPYVPYGQD
                     ATVAYGAKDFKFKNTSNSQILIWAMGVDNILYIAFYGSYIPPKIEWKHNIIETSKAPV
                     IYKTNSELSELEEKIILEGMDGAVVKSWIIIENRDGTNEIKQMGTSYYSPMPHIIERR
                     Q"
     gene            9358..>10033
                     /locus_tag="PK733_13670"
     CDS             9358..>10033
                     /locus_tag="PK733_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11625.1"
                     /translation="MTVAKKRQYREKYRKARIHLFIYSAGNKYTLRKGRFVLFLLSQA
                     FSGLGDTFKFIAITSLLVNITGSGLSAGLSLAFSIVPGILFSLFAGAIGDLLPAKFLL
                     VVIELIRSIIGLLFINNRNLAHVYILITLLSSLDAFYNPPLRKTLVNLSSEKNNQSGE
                     KDVLPGNSLLSGTFAVTSILGSLGAGIVVSKWSVRIALLLNCYFHLVAAVLLIFLRTA
                     HKECIYK"
BASE COUNT         3148 a         1831 c         1733 g         3321 t
ORIGIN      
        1 acacctatag taacatcata tatctcttta acattattcc tgccttcact ccatccgcca
       61 tttctaaggc tttccgatag cttccaaata tttttaagag acattattgt aagacctaaa
      121 gtagtttcgg caacacccat gcccaaaacc ggagctgaac ctgtaaccct tactccctta
      181 tcccacagtt cctgtgaaac tatacctttg acgcttccag cagcatgtat tactaattca
      241 aggtgaggcg cagcactaag gatatcctca ttaagatttg gtgatcccca ggatgtaata
      301 actatatttg ctccttctat taattctttt gttcgttccg gatcagggtt tgccgtatca
      361 ttattaatga tcagttcgcc tttttttgat agttcactga gagtttcctc cgagaatatt
      421 ttacctgtta attccttatt taacaatgaa actatttttt tcaagttcaa caccccttat
      481 aattactaat tttttctatt gtgtatgcac tcgtttacta ctttccataa taccaattct
      541 cattatgtac taagcataca tatactgcca catattgcat gcacacatac tgcatgtatg
      601 catactaatt catacacaat acatatacta atcatatgcc atatgtcata tgcatattaa
      661 acatataata ccatacacat attatacata atctaaccaa taatactttg caaaatatca
      721 tctttatttt gcacaattta aaaatcagca ttacctttta aaatcaattt aatactactt
      781 tgttgtgttg ccaaattggc atagtagtat taatgttttg acatcaacta ctctgccata
      841 cttgttaacg caccaagatc cattattttt acagattccc gataaaattc aataactcct
      901 tcatctctca tcctgcacat ttcacgggaa agagacggcc gtgttatatt taggaaatct
      961 gccagttcat tacgcttcat aggcataaca aacgttgtct gtctttcttt tttatactgt
     1021 tcaagaagat aactacttat cttgcctctt atgcttttca ttgagaggta ttgtactttt
     1081 tggttaagta agagagcctt tttagaaaca atattgagca tgttggatat tagatgcttg
     1141 tgacttatgc ataatttcct gcaagtaccc aaaattattt taggagttat atacatcaca
     1201 gtacaggcag tttgtgcaat tactgttgca ggccaaactt cagtccctgc aaatgctgca
     1261 atttccccaa aaatttcacc tggacccaat atatcaataa ttatgcgatc ccccgctgca
     1321 ttttctttcg ttaccgcgac gcttcctgaa agaaccagtc ccaggccgtc atatttatta
     1381 ccacttatag cggcaaattc atttttttca taactcgata tcttaggttt gaggcattca
     1441 agcatattct caagttcttc ttcatctatc ccataaaaaa gtctgcaatt taataaaacc
     1501 ttaatccatt tattaatcat gtacaccacc ttttcataga cttttacagc cgttaataat
     1561 tttaattgtt cctttaactt atttaattct ttgaattttt atagctctat gtagtttgtt
     1621 aaagcctttt tagaatatta catgcattcc tctatattgg taacttaggt taccgattta
     1681 ctgatttcat tttaatataa tgagactgta aaaacaagac aatgttttta tcaaaaggat
     1741 tttcaattat taaattacaa aatatgattt atgtaaaaac gtgaaatata gataacacac
     1801 aaaagaagct acactataaa agttttgtgc gcttatgtta cattaattaa tacgcaaaaa
     1861 tccaatttgt tccttgcaaa atcatttttg cgttatatat aggaatttaa tagtttatca
     1921 aaaatattct tgatatgtaa aataatatat aaaataggaa ggagaaatat tatgataaaa
     1981 agaaaaattg ttaagataga cgaaaataag tgcaacggtt gcggcatctg tgttaatgcc
     2041 tgccacgaag gagcactgca gctaattgat gggaaagcca agctggtatc cgaatcatac
     2101 tgtgacggat tgggtgcctg ccttcctgcc tgccctgaag aagcaattat catcgaagaa
     2161 cgtatagctg aagcatttga ctcagagagt atctgcggac aaaaagaaga aaataatact
     2221 ctggcttgcg gatgccctgg aagtcatgct aaatctttag aaagaaaaac agaaaaattg
     2281 caagcatctc cttcacagca gaaagatatg tattctgaaa ttgctgaatc acagttgcgt
     2341 caatggcctg tacaaattaa attggtacca gttaatgccc cgtattttaa gaatgctcat
     2401 cttcttgtgg cggcagattg tacagcttat gcgtatgcaa atattcataa tgattttatg
     2461 cgtaataaaa taacacttat agggtgccca aaacttgatg atattaatta tgctgaaaag
     2521 cttgcagaga tattgaaagc tcacgatata aaaagcatta cagttttaag gatggaagta
     2581 ccctgctgtg gaggtatagt ttatgcggta aagcaggcca tgttaaatag tgggaaaatg
     2641 attccatgga acgtggttac tattagtact aatggggata ttctagagga gtaaatgttg
     2701 acttcgcaaa atcactttga cttggtaaaa tcacttatat tataataaag ttaaatagtc
     2761 ccaaaggagg aataaaaatg attgaaaagc aatataagtt tatcaaaacc gacacaaagc
     2821 ttattgaaag ggtacttgaa gatgacaacg caggtattaa ccatatggtt ctgcccaagg
     2881 gagatgcttt acctgagcac ttctcaaatt cacatgttta tatgatagtt gttcgtggta
     2941 atattacatt aagactggat gaacaggaga ctcatgaata tcctgcagga agcataataa
     3001 atattcctta caacacaaag atgaatgtat caaatcagca tgatgaagtt ctggaatttt
     3061 ttgttgttaa ggctccgagt ccaagaaaat tcgcttcact taagccttaa acaataatgc
     3121 aattttcttg ttcgccaggg aaacccaggt ttccttcgac ggattgtgct ttttttagtt
     3181 tcggaggcaa taactaattt cttacaaaaa actttaggta aggggagata aatataatgt
     3241 caaacatgtt ttgttttcaa tgtgagcaaa ctgcaggagg aaaggcctgc acacaatcag
     3301 gtgtttgcgg taaaaaacct gatgtatcaa ataagcagga tgaattaatc tgcgcattag
     3361 ttggtttagc gagagcagca gcaggaaaag ttccgggaag gctcgcaaat gaacttatga
     3421 tgcaaggcct tttcgctaca ataacaaatg taaattttga taaagagagg attaatgaac
     3481 ttaaaaatca gatcctggat gaaaaggagc gacttggagg tgctgaggat ctggccttcg
     3541 atactctttg gaacggggac agtgatattg tatcacttcg ttcaacattg cttcttgggt
     3601 taagaggcat ggcggcatat gcctggcatg cctatgttct cgggaaagaa gaccctgaag
     3661 ttacctcctg gttttataag gggatgcgtg ccattggaga agaacatacc attgatgaat
     3721 ggctcaatct tcttatagga ttcggactta ttaatttgaa atgcatggag cttttggata
     3781 atgctaatac atccatttat ggtcatccgg tgcccacaag ggttactacc ataattgaaa
     3841 agggtccttt cattgttgta acaggccatg acctccatga tttaaagcaa ttgctggatc
     3901 agacacaagg aaagagtata aatatttaca ctcatgggga aatgcttcct gctcatggat
     3961 atcctgagtt gaaaaaatat cctcatttga aaggcaactt tggtactgca tggcagaatc
     4021 aacaaaaaga atttgacagc atacctgctc ctattctatt tacaaccaac tgtcttatgc
     4081 ctccaaaagc ttcatatgct gatagagtgt tcaccacagc agtggtaggc tatcctcaaa
     4141 tagaacacat acctgataga gatggcagca aagattttac ccctgtcatt aataaggctc
     4201 tagaattagg cggctgggct gaagataaac acttaacagg tattaacgga ggtacggaac
     4261 tcataaccgg ttttgcacat aatacagtat taggagttgc agataaggtt attgatgcag
     4321 ttaaagcagg agcaatccgg catttcttcc ttgtaggagg atgtgatgga gcgaaacccg
     4381 gaagaaacta ttatactgat tttgtaaaga aaactccgga agattctgtg atacttaccc
     4441 tagcctgcgg taaattccgt tttaatgatc ttaatcttgg tgagattggc ggacttccac
     4501 gtattatgga tatgggtcag tgcaacgatg catattccgc aatacaggta gctcttgctc
     4561 ttgcaaatgc ttttaattgc ggagttaatg atctgccttt aactcttgta ttatcatggt
     4621 atgagcaaaa agcggtgtgt attctgctta ctcttctggc actgggtata aagaatatat
     4681 atattgggcc ttctgtccct gcatttttct cagccaatgt tctcaatgtt ctggttgaaa
     4741 aattcggtat aaagccaatt agcactccgg atgaagacct taaagctata cttggataat
     4801 catattcgta taatcgtaag ctttatttaa tcttaatgtt caaacttaaa tgggacacat
     4861 ctgagcacct catagtgctt agatgtgtcc cattaccttt agcctaaagt caaactggta
     4921 ataaacaaac actttgaaaa gggtatgttt tcttctgtaa aattagggtt ttcccataca
     4981 acaggagaca gggtttttat tacacccggt gattaccctc tggtctcctg cgaggtttat
     5041 cagtctatgc ttaaagataa gagtgatatt gttataccta tatataataa ccttacgggt
     5101 catccggtgc ttataaataa agtagcaatt ttttatatct tttgtgtttt tcataaatac
     5161 tttgccaaaa catttttatc aactgccgat accgaatcct cgtcaacata gatatttgca
     5221 tttttgtgtg tttttagcat tgtggcggga atcatattgg tcaggtcatt ttccagtgta
     5281 atctttatag cattagcctt tactttgtat ggaacactgg ataatattac attacactgc
     5341 ataatttggt gtacagacat tgatattgcc tgcttgggta catcatctat tgttgggaac
     5401 catccttctc caacctgctg ttttttacac ttatcatcaa gattaactat tataaatggt
     5461 tttgtggttt caaaatctgc cggtgggtca ttaaatgcta tatgagcatt ttctcctatc
     5521 cctattaatc ccagatgtat aggctctctg gtaacttcct tacttagtgc aagaatttta
     5581 ctctccacat ccccttctcc atcaacaaag aatactttgc cgggattgac aatacttaca
     5641 aatctctctt tcaggtattt tctaaagctt gcaggatgag tttcaggtag gtttatatat
     5701 tcatccaagt ggaacatttc aactttgctc cagttaatat cctcttttac cagatacttt
     5761 aatgtatcaa actgggatgc ccctgtagat aaaataattc tagccttacc gtctttctct
     5821 attgcctgcc ttattatttc tgcggaaaaa gctgcagctg ccttacctaa ttcatcagaa
     5881 ttttttaata ctttaacttt catgctaatt acccccttca taatatggtg atgataatgt
     5941 atggaatagt aatcgattac tatttgctat atacacataa tatcaaaact ttggagaagt
     6001 atcaacaaca atttgcaatt ttggggacag tcctctctct tttgtaattt gggggacagt
     6061 cctctctttt ttgcattcat gtaaaatact caatcactgc ttcaggaaat ctctctttaa
     6121 taagatttgt tatgaattcc ttaatttcac cagcttgctc tttgggatac acatatttat
     6181 acctgccgaa tctaccccat ttcatggttc gatgttcttc atccatgtca agtttagttc
     6241 gaggaaatct ttcaattatt agtttttttg caacagatgt aaatctatgc tggatcaatt
     6301 caaatgttat ctcattccca gaaaattgag gacctaaacg ctctccaagt ttactgaaaa
     6361 gttcaaaata ctcttccttc caattatcat ataccattat gggagctatt ataaagccga
     6421 tagggtaacc ggcatctacc agtttcagtg cagcctccat acgctcatcc agagttgctg
     6481 tattatgttc aaaattatct attacatatc tggaattcat gcttattcta aagcgggtgt
     6541 ggccgttgtg ttttagtttt agcaatgaat ccacattact gaactttgta accactctta
     6601 gccttgcctt ctcaagactg ccaaaaaact ctatagtctt tgcaagacta ccggtaatat
     6661 gttctagaga cagtgggtca ccaaggcttg ctgcttcaaa agttgttatt tcatcctcat
     6721 cttccttaat atgctgtttt attatattaa atatatcttc tatattaacg aaaattttaa
     6781 tatagggttt attaccttga gttgtctgga gatagcaata ttcgcaattt ccagggcagt
     6841 tgcttacaag agaaaattcg aagtctgcag aaggtttaca gacatccagt tctttccctt
     6901 tcattgtagt gacaacaaga gttttttttg aatgcacata tttagatgaa gttgaattcc
     6961 caggtatata tcgaaatgca ttttttattc ctgcttctat aatttctaca ttgttgttat
     7021 tttcaaaaaa gttaaataat tccataccca ggggataatt aagagatgaa ggttcaaaaa
     7081 taaccctttc cggcatgaat aatctcatta aaccaccctt tcaaatttaa cctgcattta
     7141 atactactat gctaatttgg gaacatgaca aagtagcatt aagtcaatat atatatttcc
     7201 caattcttat tcttttatta ttatgggtgc taaatgtgac gggtagaatt tttttatata
     7261 aatttcccta tttttaagtc taaataataa gaattatacc agtaattaca aaggccgctc
     7321 ctataaatct tactaaagtt attttttcgc ccaaaaatat gcacgcaaat aaaagagtta
     7381 caaaaggtga actggacata ataattgtaa caagtgaaac atcacccctc ttaatagctg
     7441 caaaatatgc aagatcccct accagggttg ctaaaagaga ttctatagca ataagtaaaa
     7501 gcgtatatac cgggattccc ttcatctgag aaaatgttcc attggcaact gcccagcaag
     7561 ttactacact agctgcaaat aatgttctaa ataccaatcc cacagacgga ttcatatttt
     7621 ttagccctac tttggcaaaa attggtgcga tcccccagca gatcattcca aataatgcta
     7681 gcaaaaaaac tgacattctt ccttttctcc ttaaaggatt taataatttt tttattttat
     7741 tacccatttt tcccaaccta acttgcttcg cttatttgtt tggcttattt gcttcactta
     7801 tatatttttc gattgtttaa gctcttaaat agattttatt cacagatata aaaaatatgt
     7861 ccactttcgt actacaattt tttcatttaa tacagcggat tgttcatttt agctgtacat
     7921 gcaatttact aatttcatta atatattaat attatgatgt tgtattctgt taactgtaaa
     7981 aataatctgt tgcagaccaa aaaacctttg taatgtagtt tttttgtatg ttatcagtac
     8041 tatgccaatt tgggaagcaa tgttttccgg gataattttt attgtctttt cttcataatg
     8101 gctatatttc ctatatctgc ttaaatacat cctacaaaaa tgcatataat aaattgcaaa
     8161 taatatcgat tgtgtttata aaaatattta agaaatggtg gttgtctact ggtgaaaaaa
     8221 gtattaataa ttatactttc aatagtttta tttaccggaa gtattgtttt tgcaggtata
     8281 aaaagcttta acaggaaata ttcgcaacat aatgattcca gggctgaaga acaaccaaag
     8341 cctataccgg gaaatattgc atatgaaggc ggcgatgtaa cggaaatcat aaatgtcccc
     8401 ggcgggaatt cggaatcgga atcgggttcc gattctgatt ctggcactct aaatgcgatt
     8461 gcttcaggac ctgttaaaga ggttccctgg gagaatgaag atactttcaa ggaagcattg
     8521 atgcaaagtg gtgcaactac aatattggct gctttccgta cagttttaaa agacccattg
     8581 cccggtgagg aatttaatgt tcatttagca gcccgcatgc ttgcaggaac aatattaaag
     8641 cctcaggaga tattttccca aaataagctc cttggtcctt atacagaatc ccgaggttat
     8701 caggaaggac caacatatat gggttcaaga ttagctaaaa cctacggagg aggcgtatgc
     8761 aaaattgctt ctaccttgta taatgtgtca gttttgagta atctacagat aatcgaacgc
     8821 catgctcaca gcatgcctgt cccatatgta ccttatggtc aggatgctac agtggcatat
     8881 ggtgctaaag atttcaaatt taaaaacacc tcaaattcac aaatacttat ttgggctatg
     8941 ggggttgata acatccttta tattgcattc tatggcagct atatccctcc aaaaattgaa
     9001 tggaagcata atataattga aacctctaaa gcaccggtaa tatataaaac gaactcagag
     9061 ttatctgagt tagaagaaaa aattatactt gaaggtatgg atggcgctgt agtaaaatcg
     9121 tggataataa ttgaaaaccg ggatggaaca aatgaaataa agcaaatggg tacaagctac
     9181 tattctccta tgcctcatat tattgaaaga agacaataaa gttgaattaa catatggtat
     9241 tttgcttatc cagcacattt tcaggcatgt ttaaggcaca ttttcaggta tattttaaat
     9301 caaattatta tatgcttaat gtaagagtca aagtgtattc ggaaagaggt tcaagtaatg
     9361 actgtcgcca agaaaaggca atatagagaa aaatacagaa aagccagaat acatttattt
     9421 atatattcag ctggtaataa atatactcta cgaaagggaa ggttcgtact gtttctactg
     9481 tcgcaagctt tctcaggact tggagatact tttaaattta ttgctataac atcattgctt
     9541 gtaaatatta ccggttccgg gctttcggca ggacttagcc ttgcattttc aatagtaccc
     9601 ggtattttgt tttcattgtt tgcaggagct ataggggatt tactccccgc aaaattcctg
     9661 cttgttgtaa tcgagttgat acgtagtatt ataggtctac tgttcataaa taacagaaat
     9721 ttagcccatg tttatatact aattacactc ctatcttccc tggacgcttt ttataatcct
     9781 cccttaagaa aaactttggt aaatttgtcc agcgaaaaaa ataatcagtc cggcgaaaag
     9841 gatgtactgc ccggcaactc acttttaagt ggtacttttg ctgtaacaag catactcgga
     9901 tcattggggg ctggtatagt agtaagtaaa tggagtgtaa gaattgcatt actgctaaat
     9961 tgctattttc atttagtagc agcagtgctt ctgatatttt tacgtacagc ccataaagaa
    10021 tgtatctata aaa
//
LOCUS       DAOSDD010000168         9999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2553_length_9999_cov_4.957965, whole genome shotgun sequence.
ACCESSION   DAOSDD010000168 DAOSDD010000000
VERSION     DAOSDD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_9999_cov_4.957965"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK733_13675"
     CDS             <1..476
                     /locus_tag="PK733_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HOJ11626.1"
                     /translation="LSLISKGKANSSNFIGEMRNYASKLVSTIISSSETYKHDNITKE
                     KCPECGKYLLEVNGKKGKMLVCADRECGFRKTVTQISNARCPECHKKMEIRGEGENKT
                     FFCNCGYREKLEAFKERKSEQVSKREVNKFLRQQDTSELSNTSLAEALAKWKTKA"
     gene            complement(558..7673)
                     /locus_tag="PK733_13680"
     CDS             complement(558..7673)
                     /locus_tag="PK733_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11627.1"
                     /translation="MKKLICFITAIIFAANLVFPTFAYAAEDTGIRVHNGIVEYRVNS
                     ENGRFTIHTADGLPNKASDNDKNLLFFWDKPNTSFTTFRIDGKDYIFGNSYGLLGAEG
                     GIVSPPKVDGNITTTVWRIKDIEVTQKLQLIVDMSNPNVGNTKITYQILNKGTSNVQL
                     GSRILLDTQLGTNDASSMLVGANYVTNETEYIGKGIPAAWKSADEKFAPGVISYGLLS
                     GWDNIAPDRMVIAHWESLSNTKWGYAPNELINFTSNKNKYGSADSAVALYFEPKTLNN
                     GAEVVYETFYGIGSLSDTYNDAKFNFQINTPNKLTVNKEGTGYNEENFNVVVTIDNSS
                     ASAEPITNATVILGLSEELSFVDDQPDRQYIKNIAAGETLAVEFKVSAKVQQSLKVAE
                     LGATVQYNNQYNNNESYAEGRKFIVLPSVKGKPPAMQMTEISPKIAYTKSQKKTFVIK
                     GSDFDALKADYEWTMYIKGQNSGISYQIMRKDINITNDTLTVAINKDYEFKADKYNIV
                     LYSSAYGNMSKTIELSNNKKYDRKEYGTLLMGAFKEDKYGKPVYDVMVLEKEEEFEKL
                     DDSIKDNILLTLRGEIYEYEINGQIQYDCGSGTIINRSILYTAPTAEPNKCLTVKRYN
                     KGGVDWWGRIEDSLVLTGDGFLTIGDYTFHYGDFYVVLKDGEEYELADPTDAEGDSDY
                     NWDNPMEDEKSNVEIITPTNVVAGEILKTVGLFTGTKITINNAVIGTNTVSLGGSISV
                     ELPWWSSASDDGDDDEGDDEGKSDLEKKFDKNDGLNSAVGEAKKNDDLLSLSLDEMRY
                     GVTNNNSSYLVGLIANGSLQLDDDTIPKFKAGGAHAAFALDSLNYDGMYAAINAGFKV
                     GDAFECDGTVALVFETGGSCIPDSVELVLGGDALKIPLGAGPANVGYLTKIGGGVYNL
                     YNTIKGYYNVIPPLTLKLISGYADPTLYSFRLDTIGLEFGLNGFQLTAEEGKIVGIKM
                     IDSAYAKFLVYVTEYNGVKYPCVDIGAGIKMNILGIIRGEGSIWLVADPRIDSIFGHV
                     SLGGKAYVGLFIPDYIPLLGGMELAAIMAELSTYRAYLGIRIIGIPFSVGYYWADKKV
                     KFNDDFALLAQELNVPMEELENALGYEYVGTDSNSDGLLVFGGNLRQTYSSKRSNDAK
                     LLAYDTKHEIPVNSQDYALFELQYTGDMPSITVTKPDGSNYVLKENENYRVQTIAAEN
                     TDSGITEQMVYISVTDPEDGIWTITTDKAVDCTAMNVLALPELESVSCQQVSNQQLKV
                     GWDALNVDDSYTVDINLSEIQKTVDLEDFSGNEEEYNREMAQGYDPGIIVAKDIPVND
                     KQALVDITERLTSGSYQARVVLKKGNEVYASELSQETFTYVNPNVPDMPENIQVSPGG
                     DGQFKVTYDAVENAQGYVVTILDESGNELEGFEGIITDKTKVYIGNKSTVAEGYDEKG
                     NPTGFKEAGVATGKSYRVKVYSYNEKDYITYKSQEYVSDVIYLPQPNPAKVTLKLNDT
                     LATEADNKNKNTVGATTNNKNSVVKYIPDQNVEVAYWVDGVTNDQIYEVDANAPLEIP
                     VTLDEGGSVVEFLAVNGANDYTSSKLIATLDTVNPELLLDSVSVLSTNGRYEIVGTSE
                     PNANVYVHIGDNLNAAPAPIPMFAPTSVSASVPIPVPVINGSFSYIGRQTGTRDEIKV
                     TAIDAAGNETVMYVEIIPSEISSFKSISIKNDEYKEVENIEIFQGDTAQIKVYGVSEN
                     GEEFQLDNRNIAYSVVYGCDKASINEEGVIEGNYYGEAVVLCEYYVTDNYSFEQTVNV
                     TVSQAFEEAKDIRISTTDVSASQVGSLVARFSIPDAPVGVTYSYTMVDNDYFELAGNQ
                     LILKKEMDASAVTILVTAQGKHVLDGIYENIGNPITKEFTLKLVKNIESAEIFDSITV
                     YEGTPFKSLRLPEKAKVKLNTGELIYYPITWNRGAYNADIVKTYNLKGTIQRDKNVNN
                     PDDIYAQISVTVREYPPEEETITDTLKKDKTTPKDDITIPEEDITYKYEDGTMVINVK
                     KGINTIKLGETILGMKTDKLKINFENIEFVLDNDTVDALRNIIDKSTNTNNAYVSISA
                     KLEGNSISINVENLENVKGIVRAKLPDQGEASESGKMSEQGEISKHGDAPKLEGAFKP
                     EDINEYNMVILKDGKVVPNSYYDKTTGRIVFKMDGNGDYTAEQRSFRFDDTKDNWAKD
                     IISYAAVRGLVNGVGNNNFAPNNNLTRAEFLKMILGAAGIIPETTVTVNTKLASDVKA
                     TDWYAPYVSYAMEKGIVSGPGDGSFRPNNYITRQEMMVILNNCMKYLGFDLKTKGAGE
                     LNFKDKSNVSDWAIAAVQNVTSLGLVVGTDDGRLLPLNNATRAEGAAVMMRCIKLMAN
                     HIEDCIQ"
     gene            complement(7688..>9999)
                     /locus_tag="PK733_13685"
     CDS             complement(7688..>9999)
                     /locus_tag="PK733_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11628.1"
                     /translation="KTRTAQNVVATITFDETTTITNNGGSANYTFFENGSFTFEFRDS
                     LGNSSTAVAAVNWIDRAVPTSKVIVTNGSGDIIGDKWTNENVIVSIIPSVNSVVEDVK
                     YNDALVGNSSLIADLGNNQYQVSGYGRLTYTVKDKDTYLTNTGEALIRVDKTAPKVKN
                     IVYSTQNWTNQNVTVTISGEDDLGTVTYPNGSSHIFTENGSYEFIIKDNAGNKTSEIV
                     NVDWIDKKVPTPVVTYYVEGKEYDTKAPINKNVVAKVTFDGDGSPFNVNGDGSPVFVT
                     NNNGSTEYEFNGNGSFTFMFRDEAGNEGSVVAMVSKIDKVAPTGYVAYSYTGWTNSDV
                     IATLVATDDMNDVIIINNPSDQYTFTENGAFTFEFKDSAGNVGKATAMVTKIDKTPPK
                     LSYKLSTEEKTALSVYAFVEADETVTILNNDGKTSRQFNSNGEFTFIAKDRAGNVSEM
                     AVTVSNISKETTPVVLIYSNMDPTNGDVYVTIAPEDGSSYIYVTNNNGQKIKKFTENG
                     EFIFTYKNSVGVEGEAAASVSNIDKTPPNVGVDYSHIEITKENVVATFSSDKEVTYPY
                     IVVDNKYTFTENMKLLIPVEDKVGNKVNVIIETNLIDKKAPEITFENQYEVLKLGSKF
                     NVEEGVTVTDDTALEGEAAISGDYNANIAGDYVITYTATDKAGNVSKAYKYLTVYDPN
                     KFNVIVNGRMTSMGQITVDSKDIVIKTINSSGKVKAKILPSKKNIGDFKTKGEVIELV
                     SIFPSQGYYTLYITDDERNSRLVYLFIAE"
BASE COUNT         2846 a         2125 c         1574 g         3454 t
ORIGIN      
        1 agctttcatt gattagcaag ggtaaagcaa attcaagcaa ctttataggc gaaatgagga
       61 attatgcttc caagctggtt tctaccataa tatcaagttc agaaacttat aaacacgata
      121 atattacaaa ggaaaagtgc cctgaatgcg gaaaatacct tctggaggtt aatggcaaga
      181 aaggtaaaat gcttgtatgt gctgaccgtg agtgtggttt ccggaaaaca gtcactcaga
      241 tttcaaatgc aagatgccct gagtgtcata aaaagatgga gatacggggc gaaggagaaa
      301 ataaaacatt tttctgcaat tgcggttata gggaaaaatt ggaggctttt aaagaaagaa
      361 aaagtgaaca agttagtaaa agagaggtta ataaattctt aagacaacag gacacaagtg
      421 aattatcaaa taccagcctt gcagaagcat tggcaaaatg gaaaaccaag gcataaaact
      481 tgtacctggg ttgccacatg taaatgtagc aacccaggta caaatttcgt tatcttacaa
      541 aatcccacaa ggatttttca ctggatacag tcctctatgt gatttgccat aagtttaatg
      601 catctcatca taaccgccgc accttctgca cgggtagcat tgttaagtgg caacagcctt
      661 ccatcatcgg tacctacaac cagcccaagt gatgtaacat tttgtactgc tgctattgcc
      721 caatcagaaa cattgctttt atctttaaaa ttaagttcac ctgcaccctt tgtctttaaa
      781 tcaaatccca aatacttcat acaattatta agtattacca tcatttcctg tctggttata
      841 taattatttg gcctgaagct gccatccccg ggtccgctca ctatgccttt ctccatagca
      901 tagcttacat aaggtgcata ccagtctgtt gcttttacat cagatgccaa ttttgtattt
      961 actgttaccg tagtttcagg aataattcct gcagccccca gaatcatttt caagaattct
     1021 gctcttgtca gattattgtt gggtgcaaag ttgttatttc caacaccatt tacaagccct
     1081 cttacagcag catatgaaat aatatctttt gcccagttgt ctttggtgtc atcaaaacgg
     1141 aagcttctct gttctgcagt ataatcacca ttgccgtcca ttttaaatac tatcctgccg
     1201 gtagttttgt catagtatga gttgggcaca acctttccat cttttaaaat caccatgtta
     1261 tattcattta tatcttccgg cttaaaagct ccttccagtt ttggtgcatc cccatgtttc
     1321 gatatttctc cctgctctga catttttccg gactccgatg cttctccctg atccggaagc
     1381 ttagctctaa caatgccctt aacattttca agattttcta cattaatgga aattgaatta
     1441 ccttccagtt ttgctgaaat gctcacatat gcattatttg tatttgtact tttatcaatt
     1501 atatttctaa gtgcatctac ggtatcatta tccaaaacaa attcgatatt ttcaaagttt
     1561 atcttaagct tgtcggtttt cataccgaga attgtttcac ctaattttat ggtatttatg
     1621 cctttcttca cgtttataac catagttcca tcttcatatt tataggttat atcctcttct
     1681 ggtatagtaa tatcatcttt cggagtagtt ttatcctttt ttagtgtatc tgtaattgtc
     1741 tcttcctcag gaggatattc ccttaccgtt acactgattt gtgcatagat atcatccggg
     1801 ttatttacat ttttatctct ttgtatagtt cccttcaaat tatatgtttt tacgatatca
     1861 gcattatatg cgcccctgtt ccatgtaatg ggataataaa tcaattcgcc tgtatttaat
     1921 tttacttttg ccttttccgg cagtcttaaa cttttaaaag gagttccttc gtaaacagtt
     1981 atactgtcaa atatttctgc tgactcaata tttttaacaa gcttaagtgt aaattccttt
     2041 gtaataggat tgcctatatt ttcataaatt ccgtcaagaa catgcttgcc ttgtgcagta
     2101 actaaaattg taacagctga tgcatccatt tcctttttta gtatcagttg attccctgca
     2161 agttcgaagt aatcattgtc aaccatagta taactgtaag ttacacccac aggcgcatca
     2221 ggtatggaaa atcttgcaac aagcgatcca acttgtgaag ctgaaacatc agttgtagat
     2281 attctgatgt cttttgcttc ctcaaaagcc tgtgatactg ttacatttac agtttgttca
     2341 aaactataat tgtctgttac atagtattcg caaagtacga ccgcttcacc ataataatta
     2401 ccttctatca caccctcctc attaatagat gctttgtcac agccatatac tacactatat
     2461 gcaatatttc tattatcaag ctggaactct tcaccatttt cagagacacc atacacttta
     2521 atttgagctg tatcaccctg gaaaatttca atgttttcaa cctctttata ttcatcattc
     2581 ttaatggaaa ttgatttgaa gcttgatatt tcagaaggaa ttatttcaac atacattaca
     2641 gtttcattac ctgctgcatc aattgcagta actttaattt catcacgagt accagtctgc
     2701 cttccaatgt atgaaaagct cccgttaata acaggcacag gtatgggcac agatgcagat
     2761 acagatgtgg gtgcaaacat aggtattggt gcaggggcag cattcaaatt gtcacctata
     2821 tgtacatata catttgcatt gggttcactt gttcctacaa tttcatatct gccatttgtg
     2881 cttaaaacac ttactgaatc aagaagcaat tcagggttta ccgtatccaa tgttgcaatc
     2941 agttttgacg aagtatagtc atttgctcca ttaaccgcca gaaattcaac aacactgcca
     3001 ccttcatcaa gtgttacggg aatttccaaa ggcgcatttg catcaacttc ataaatttga
     3061 tcatttgtta caccatcaac ccaatatgct acctcaacat tctgatccgg aatatatttt
     3121 acaactgagt ttttattatt tgtggttgca ccaacagtat ttttattttt gttgtcagcc
     3181 tctgttgcca atgtatcatt taatttaagc gttacttttg caggatttgg ctgaggcaga
     3241 tatataacat ccgagacata ttcctggctc ttgtaggtaa tataatcctt ttcgttatat
     3301 gaataaacct taaccctata agatttgcct gtggcaacac ctgcttcttt aaaacctgtt
     3361 ggattgcctt tttcgtcata accttcagca acagtcgact tattgccaat ataaaccttt
     3421 gttttatcag ttattattcc ctcaaaacct tccaattcat taccactttc atcaagaata
     3481 gttacaacat atccctgtgc attttccaca gcatcatatg ttaccttaaa ttgtccgtca
     3541 ccacctggtg aaacttgaat attttcgggc atatcgggaa catttgggtt tacataagta
     3601 aatgtctctt gtgataattc acttgcatat acctcattgc ccttctttaa tacaactctt
     3661 gcctgataac tgccgcttgt taatctttct gtaatatcaa caagagcctg cttatcatta
     3721 acagggatat ctttagcaac tataataccc gggtcataac cttgtgccat ctctctgttg
     3781 tattcctcct cattgcctga aaaatcttcc agatcaactg ttttctgtat ttcagaaagg
     3841 tttatatcaa ctgtataact gtcatcaaca tttaatgcat cccagccaac tttaagctgt
     3901 tggtttgata cctgctggca tgatacactt tcaagctcgg gaagtgccaa aacgttcata
     3961 gcggtacaat caacagcctt atcagttgta attgtccata ttccatcctc cgggtcagtt
     4021 acagaaatat ataccatctg ctctgtaata ccgctgtctg tattttcagc agcaattgtc
     4081 tgaacgcggt agttttcatt ttcttttagc acataattgc tcccatcggg ctttgttact
     4141 gtaatgctcg gcatatcacc tgtgtattga agctcaaaga gtgcataatc ctggctatta
     4201 accggtattt catgctttgt atcatatgcc aggagttttg catcgttgct tctcttggat
     4261 gaatatgtct gtcttagatt ccctccaaag actaaaagac catcactatt tgaatcagtt
     4321 ccaacatatt cataacccaa agcattttca agttcctcca tgggtacatt taattcttgt
     4381 gcaagaagag caaagtcatc attaaattta acttttttgt ctgcccaata atatcctaca
     4441 gaaaatggaa tacctataat tcttatgccg aggtaagccc tatatgtgct gagttccgcc
     4501 attatagcag caagctccat accacctaaa agcggaatgt aatccgggat aaataatccc
     4561 acataagctt tgccacctaa agatacatgc ccgaaaatgc tgtcaatcct gggatctgca
     4621 acaagccata ttgatccttc acctctaata ataccaagaa tgttcatttt aattccagca
     4681 ccaatatcaa cacaaggata cttcacacca ttatattccg taacataaac aaggaatttt
     4741 gcataagcgc tatcaatcat cttgattccc acaattttac cttcttcagc agtaagctgg
     4801 aaaccattta ggccaaattc caagccgatt gtatcaagcc ggaaggaata aagtgtagga
     4861 tccgcatatc ctgaaatcag ctttaatgta aggggaggta ttacattgta atatccttta
     4921 atggtattat acaggttata tacaccgcca ccgattttgg tcaggtaacc tacgtttgca
     4981 ggacctgctc caagaggtat ttttaatgcg tcaccaccca atacaagttc tacactgtca
     5041 ggaatgcagc tgccacctgt ttcaaaaaca agtgctaccg ttccatcaca ttcaaaggca
     5101 tctcctacct tgaacccggc attaatcgct gcatacatgc catcatagtt taaggaatcc
     5161 agagcaaaag cagcatgagc accccctgcc ttaaatttcg gtatggtatc atcatcaagc
     5221 tggagactgc cgttagcaat aagaccaact agatatgaac tattattgtt tgtcacgcca
     5281 tatctcattt catcaagact aagtgacaat aaatcatcat ttttctttgc ttcacctaca
     5341 gcactgttta aaccatcatt tttgtcaaat ttcttttcca aatcactttt tccctcgtca
     5401 tcaccttcat catcatcacc atcatctgaa gccgatgacc accagggaag ttcaacagaa
     5461 atactgccgc caagggatac tgtatttgta cctataaccg cattgttgat tgttattttt
     5521 gtacctgtaa ataatcctac agtttttaga atctcacccg caacaacatt agtcggcgta
     5581 ataatttcca catttgattt ttcatcttcc ataggattat cccagttgta gtcactatca
     5641 ccctctgcat cagttgggtc ggcaagttca tattcttcgc catccttaag cactacataa
     5701 aagtcgccat agtggaatgt atagtcacct atggttaaaa atccatcccc cgtaagcact
     5761 aaagaatcct caattcttcc ccaccaatca actccgcctt tattgtatcg cttaactgta
     5821 aggcatttat taggctctgc tgtaggtgcg gtgtataaaa tggaacggtt tataattgtg
     5881 ccgcttccac agtcatactg aatttgccca ttaatttcat attcgtatat ttcaccacga
     5941 agtgttaaaa ggatattgtc ctttatactg tcatctaatt tttcgaactc ttcttctttt
     6001 tccagaacca ttacatcata taccggcttc ccgtatttgt cctctttaaa tgcacccatt
     6061 aaaagtgttc catattcttt tctgtcatac tttttgttgt tggagagctc aatggttttt
     6121 gacatattgc cataggcaga tgagtaaagg acaatattat atttgtccgc cttgaattca
     6181 tagtccttat taattgcaac agttaaggta tcatttgtta tgttgatatc cttacgcatt
     6241 atttgatagg aaataccact gttctgcccc ttaatataca tcgtccattc atagtcggct
     6301 tttaaggcat caaaatcact gccctttata acaaatgttt tcttctgtga tttggtatat
     6361 gcaattttgg gtgaaatctc ggtcatttgc atagcgggag gcttcccttt aaccgaagga
     6421 agaacaataa attttctccc ttctgcatag gattcattgt tattatattg gttattatat
     6481 tgaactgttg ctccaagctc tgcaactttt aatgattgct gaacctttgc tgaaacctta
     6541 aattcaaccg ccagagtttc acctgcagca atattcttta tatactgtct gtccggctgg
     6601 tcatcaacaa atgaaagttc ctctgataaa cctaaaatta ctgttgcatt tgttattggc
     6661 tcagcactgg cactgctgtt atcaatggtt acaacaacat taaagttctc ttcattatag
     6721 cccgtgcctt ccttgtttac cgtcagttta tttggagtat ttatctgaaa attaaattta
     6781 gcatcattat atgtatcaga caaacttccg ataccgtaaa aagtttcata tactacctct
     6841 gcgccattat taagtgtttt aggttcgaaa tataaagcta cagcgctgtc agcacttcca
     6901 tatttatttt tatttgaagt aaagtttata agctcatttg gtgcataacc ccatttagtg
     6961 ttggataaag actcccagtg agcgattacc attctgtcag gagctatatt gtcccagccg
     7021 cttaaaagcc cataagatat aacaccgggt gcaaattttt catctgccga tttccatgca
     7081 gcaggaatcc ccttaccaat atattcagtt tcatttgtta cataatttgc gccaaccaac
     7141 attgaagatg catcgtttgt gccaagctgg gtatcaagca atattcttga accaagctga
     7201 acattacttg tgcctttatt tagaatctgg taggttattt tagtgttacc aacattagga
     7261 tttgacatat caacaatcag ctgaagcttt tgtgttacct ctatatcctt gattctccat
     7321 acagttgttg taatatttcc atcaacttta ggtggtgaaa caatacctcc ctctgctccc
     7381 aaaagtccgt atgaatttcc gaaaatatag tctttcccgt caattctgaa ggttgtaaaa
     7441 gaagtatttg gcttgtccca aaagaacaac aaatttttat cgttatcgct tgccttattt
     7501 ggaagtccat ccgctgtatg gattgtaaat ctgccatttt cactgttaac tctgtattca
     7561 acaataccat tatgaactct tatgccggta tcttctgctg catacgcaaa tgttgggaaa
     7621 acaagatttg cagcaaatat tattgctgtt ataaagcata ttaatttttt catcttaaga
     7681 ttacccccta ttctgctatg aataaataaa caaggcgaga attcctctca tcatcagtta
     7741 tgtataacgt gtagtaacct tggctcggga atatgctcac aagctctatt acttcacctt
     7801 tggttttaaa atcgccgatg tttttcttgc ttggcagtat ttttgcttta accttgccac
     7861 ttgaatttat tgtttttata acaatgtctt tagagtcgac agttatttgt cccatgcttg
     7921 tcattcttcc gtttactata acattaaact tattaggatc ataaacagtc aaatatttat
     7981 atgcctttga aacattacct gccttatcgg tggcggtata ggttataaca taatctcctg
     8041 caatatttgc attataatca ccgctgattg cagcttcacc ctcaagagca gtatcatcag
     8101 ttaccgtaac accttcttct acgttaaact tactaccaag cttaagaacc tcatactgat
     8161 tttcaaaagt aatttcaggt gcttttttat caatcaaatt agtttcaata ataacattaa
     8221 ccttgttgcc taccttgtcc tcaactggaa tcagaagctt catattctct gtaaaggtat
     8281 atttgttgtc gaccacaata tacggatatg taacctcctt atccgaacta aaggttgcaa
     8341 caacattttc ctttgtaatt tcaatatgcg aatagtctac gcctacatta ggcggagttt
     8401 tatcgatatt tgaaacgctt gcagccgctt caccttcaac acctacggaa tttttataag
     8461 tgaatatgaa ctcgccgttt tctgtaaact tctttatttt ctgtccgttg ttgtttgtaa
     8521 catatatata tgaagaaccg tcttcaggtg caattgttac ataaacatct ccatttgtag
     8581 gatccatatt tgagtatatt aacacaacag gagttgtttc ttttgatata ttggaaacag
     8641 ttaccgccat ttcacttaca tttcctgctc tgtcctttgc aataaatgta aattcgccat
     8701 ttgagttaaa ctgccttgag gttttaccat cattatttag aatagttact gtttcatctg
     8761 cctcaacaaa agcatagaca ctgagagctg ttttctcttc agtcgacaat ttatatgaca
     8821 gctttggtgg tgttttatct atttttgtaa ccattgcggt tgctttgcca acgtttcctg
     8881 cactatcttt aaattcaaat gtaaatgctc cgttttctgt aaatgtatat tgatccgacg
     8941 gattatttat aataataaca tcattcatat catctgtagc aaccagtgtt gcaattacat
     9001 cactatttgt ccagcctgta tatgagtatg caacataccc ggttggtgca actttatcta
     9061 tttttgatac cattgccacg acgcttcctt cgttaccggc ctcatctcta aacataaagg
     9121 taaatgaacc gttgccgttg aattcatatt ctgtgctgcc gttattattc gttacaaata
     9181 ctggagaacc gtccccatta acattaaatg gagaaccgtc cccgtcaaag gttacttttg
     9241 caactacatt tttatttatg ggagcttttg tatcatattc ctttccttca acataatagg
     9301 tcaccacagg tgtaggaact ttcttgtcaa tccagtcaac atttactatt tcagaagttt
     9361 tatttcctgc attatctttt atgataaatt catagctgcc gttttcggta aaaatatggc
     9421 ttgatccatt aggatatgtt actgtcccaa gatcgtcttc acctgaaata gttactgtaa
     9481 cattctggtt tgtccagttt tgggtggagt aaacaatatt ttttaccttt ggcgctgttt
     9541 tatcaactct tatcaatgct tcaccagtat ttgtcaaata tgtgtccttg tcctttacag
     9601 tatatgttaa tcttccatat cctgaaactt gatattgatt attgcccaaa tcagctatta
     9661 ggcttgagtt acctactaaa gcatcattat atttgacatc ctccacaaca gaatttaccg
     9721 atgggataat acttacaata acattttcat ttgtccattt gtctccaatt atatctcctg
     9781 aaccgtttgt tactatgact tttgaagtag gtactgccct gtcaatccag ttaactgctg
     9841 caacagcagt agaagaattg cccaaactat ctctaaattc aaatgtgaaa ctgccgtttt
     9901 caaaaaatgt atagttggca ctgccgccat tatttgttat agttgttgtt tcatcgaatg
     9961 taatggttgc aacaacattt tgtgctgttc tggtttttg
//
LOCUS       DAOSDD010000169         9996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2554_length_9996_cov_5.135399, whole genome shotgun sequence.
ACCESSION   DAOSDD010000169 DAOSDD010000000
VERSION     DAOSDD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_9996_cov_5.135399"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PK733_13690"
     CDS             complement(<1..1149)
                     /locus_tag="PK733_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11629.1"
                     /translation="MQGRTEINNRKMFTKRNKAVKQTAAESRLTPAGLTPIETLKRLI
                     FISKEYIPWYIGLLIAAIAVSATGLLDTEGLRRIVNGTTTGDKNLLVNGVIISLIALG
                     TDQVIEFTRGYFGEILNFISIRNLQSALISKLTRALMKNYDSYHTGDLIDRISNSSEQ
                     AQSGLNNNAVNILQNFVLLVFIIVYLVSVNIKMTLASIIFIIVLPLIVNPLSKKLRAL
                     HEENQKVSAEKSAFIQDAVQGAEVVRVYLLTDRLAQSLNKIYEKLVAVLKKLIPYESF
                     MYSTHVMVIVTGDFFILGFGGYLVSRGELQVGDVIAFLILFERMFQPLSYISTVWPQF
                     QSSIAAANRVFEVLNLPEETDAANVSPIPEEGDICFKDIFFSYSGKDNG"
     gene            complement(1133..2017)
                     /locus_tag="PK733_13695"
     CDS             complement(1133..2017)
                     /locus_tag="PK733_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11630.1"
                     /translation="MSSEKIEGIGSFYGGEYDSIAVEGIGKLKGDAAAKSVVVEGLFK
                     GKGRLITDQMVVEGVARCFRNIKSKYTEINGVLKLRRADLNADKIVCDGIIVATREVS
                     ADEICIDGSCSVAKMYGDKITITNNINSWKDSKIPIKFMFLMMLYFGRKISLSHCLVD
                     ILECTDLTASGLKSKIIRAGSVKLKDHCIVGKLICDGEIIIDDTCQIGEIISNNSIQI
                     ESNNKAQVEPEGKTHMESKSKTQVEKEELKIKEIKANTNAVKIMDLYKNGKISADEAE
                     KMLAACGCAGGIYNAGQN"
     gene            complement(2014..2856)
                     /locus_tag="PK733_13700"
     CDS             complement(2014..2856)
                     /locus_tag="PK733_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11631.1"
                     /translation="MRRNMVVLGNRSIQGSFGRILSAGEIIIKDSTIKSLYCAGDTDI
                     KNSSAEKVRCAGDIDASSTNFISFKAAGDVNLKGICKAETAVITGSLNAELFQCNVLR
                     NSSRNSKINGVLEWSGGLQAETFESLYSFTLNCDYEFKNIISSAILNCNNEIVCENFY
                     SFGGLSAQALNAENIFILINDDISLECIVGSNIRISNTFNPDKKFKNIPKSASYGKLA
                     GSGNIIIVNVIEGDNIIIENTKSELVSGMDVFIGDLCIIEKVEYRNSVRISEKAVVNE
                     VVKI"
     gene            complement(2853..3548)
                     /locus_tag="PK733_13705"
     CDS             complement(2853..3548)
                     /locus_tag="PK733_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HOJ11632.1"
                     /translation="MLISKKDLLNETGISYGQLYRWKREGLIPEDWFIKKPSFTGQET
                     FFPRTKILNRIRAIQELKDKYSLEELAKILSPEVAERNFTTDDLNIIEEIQKGLIPAF
                     VKGFDKNNFTYIEVLIMIALSDFKRDCDITIGQIIEIAEGLRGYLSNMKSTGYIFVLF
                     DKNKDYYATIYTEQAQVYLDIRLNIVKSIHLDEISNRLKLKYRKKFNFKFDNEEDENT
                     VFISACEPKGVTI"
     gene            complement(3626..6739)
                     /locus_tag="PK733_13710"
     CDS             complement(3626..6739)
                     /locus_tag="PK733_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ11633.1"
                     /translation="MDINIFSFNHPLILLVIIPAAALTIYLLRNMGGLSNSKRKLTGA
                     IRLIVLVLLIFAISGFNIRQVSNSTTTIFALDISDSTYRHKTEIEDFVKDAFKNKKHQ
                     EKAGIVVFGANATVETIPTANPFFDSIQSKINANYTNIEQALGFASSLIPGSDRKRIV
                     LVTDGEENMGDSLKLAGLLNNKGIVVDVMHVKQQKSQEVNEVQIKEVTIPEVLHKGEK
                     FEVTVKVDSTVKTKGSLKLYADRQLAAERSVEIDEGMNTFVFSGTAGNGGLVVYTAVI
                     EPEDDGVIKNNSMAAFSHIKDIPEILVIQNKSGNVAGNALGSASELIRIFEKDVKVRT
                     VEPENVPKSLEDMLMYQAFIISDVSAEELDERFLNNLEICIKHQGKGLLVTGGENSYA
                     LGGYYETPLEKVLPVNMDITPKEESPNLGLVLVIDKSGSMTGGQYGVSKVELAKEAAI
                     RSTEVLTPRDMIGVIAFDSAAQWVVKTQKLDNLPKIQDDIGTIRAGGGTQILPPLEEA
                     YLSLKNADTKLKHIILLTDGEAEKTGYEPLIDKINAAGITLSTVAVGQSADMQLLNAL
                     AAGGKGRFYMTDEFSHIPKIFAKETFLAGKTYLNNRVFAPGLKSQSEILKGIDSIPVL
                     EGYVGTTAKNTAQVVFASDTDQPILAVWQYGLGRTAAWTPDVKGAWTRNWMKWEQSPL
                     FWKNVLSWIIQRHSIDDYSIKGGLYENYSTDRSTDKGQIKDAGGIGAIEVELPNINDE
                     PISEPMPESILESISGQVSGQVTGQVIGQVTKPASGPVIGPVIEPVIEAVIVSPSGKE
                     QQFRLQPISRIKYEGIFQADETGIYLASINVKYGTEIVKNINTGISIPYSPEYNISRV
                     DNLAFLQKLAYEGGGRILGSGAEVFKGDPPPVAGNKDMTNILIALSIIVFVLEIGIRR
                     LNLKLDAATERMTQAAHVIQETKRHIKYKYVKNAFPKPGSMEQKDSTVQYVHEEVNIR
                     NPKIKGKNTTIDNTSINENIDNDNKNEYNEKKDNKDNKYNKDNASSHLSLLLEKKHRR
                     NKNSKKF"
     gene            complement(6729..8780)
                     /locus_tag="PK733_13715"
     CDS             complement(6729..8780)
                     /locus_tag="PK733_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOJ11634.1"
                     /translation="MKFYSPYAFIYLISIPVIILFYLLKQQYREHTVSSLYLWQEVLK
                     DVEASTPWQKLKKNLLMILEITAALFLVFALANPFINAGGKKVKKVAILIDTSMSMQA
                     VDVKPSRFEAARKLAGDYLTRLEPETQITLISMGSKAMIEENMSSDKARVADKIKALK
                     VTNGISNFSEAASIINSLWEVDPDVNVICFGDKEFIVGEGNQNIEFVKVMGEGSNFAI
                     THMSHTLLKDNSISLLVRVANFGENDETLPMSLYADGKVADAREVVIKAGERANVYWS
                     RIPDNARVLECRIDRDDSLKADNTAWTVINSSEKARVVMITKGNLFLEKVLKLMGNID
                     LYKGSIEDIQELKGYDLYIFDNIFGNTDSDSAGSKDNTGSKDNTGSRDSAGRINSGKL
                     SLDELPLDGNILIFNPPKNDIFTVKGEAGLPQTEKTNHELFEHINDYNFSIYKSKLFD
                     VPVWGDEVLGSDKGTIAFAGEIDKRRLLVFGFDLHNTDMVLKSAFPIMMTNAIEWLVP
                     PAVKSIGSIYPGLEIEFSLNPKTEEALVLTPSGESIVIAPPFPVKAFDYTHEPGVYIL
                     EQDNRIRSYFAVNVPAEQEGDIFEREVEKPEQTWEAKDAGTGIGGQTGINDMAASDKA
                     AKTDEETKTIQTGETSGTLGTLDTSGTRNQSAGFSLRNIMLWIVLLLLCVEWWVYTNG
                     Y"
     gene            complement(8898..9773)
                     /locus_tag="PK733_13720"
     CDS             complement(8898..9773)
                     /locus_tag="PK733_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ11635.1"
                     /translation="MSELFDSDFLNKIQKLTLSTGIIVSGGAGGNRKSRQKGSSVEFS
                     DYREYSEGDDFRRIDWNAYGRFEKLYVKLFMEEREAPVNIFLDTSKSMDWGEPNKSFA
                     SRRLAAALGYMSLSAYDRVSVICLSDRVEKSRLSLRGKHFLPELLTFLEEVEYKNGPG
                     FYEAIKAYNLKDNRGISIIISDLFSPGGLTEMIKYLQYRKQDVYICQILAPWEARPDI
                     GTNMRLVDSETGRTIDVTATPRLLKSYNRIFNDFIANIREECFRKGAAYYMMDTAMPI
                     EQMISMITGLNKVRS"
     gene            complement(9770..>9996)
                     /locus_tag="PK733_13725"
     CDS             complement(9770..>9996)
                     /locus_tag="PK733_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ11636.1"
                     /translation="YIRFGSSPRGAQAVVSTARIKAILDGRYNVAFDDIRYAAYPALR
                     HRIFLNFEGISEGLTTDDLIKTIIEEIEKS"
BASE COUNT         2829 a         2184 c         1503 g         3480 t
ORIGIN      
        1 tccgttatcc ttacccgagt agctaaaaaa gatatcctta aaacaaatat ctccttcttc
       61 aggtattggt gaaacattgg cagcatctgt ttcttcaggt agattcagaa cctcaaatac
      121 cctgtttgca gccgcaatgg aagactgaaa ttgcggccac actgttgaaa tataagacaa
      181 cggttgaaac attctttcaa aaagaataag aaaggcaatt acatctccaa cctgcaattc
      241 tcctcttgaa accagatatc ccccaaaccc cagaataaag aagtcgcctg taacaattac
      301 cataacatga gtagaataca tgaagctttc atatggtata agttttttta gtactgcaac
      361 aagtttttca taaattttat tgagggattg ggcaagcctg tctgtcagaa gatacaccct
      421 tacaacttca gcgccttgaa ctgcatcctg tataaatgcg cttttctcag cactcacctt
      481 ctggttttct tcatgaagtg ctctgagttt ttttgataag ggattgacaa taagcgggag
      541 aactataata aatattattg atgccagtgt catcttaata ttcacactta caagatacac
      601 aattatgaaa acaagcaata caaagttttg cagtatattc acagcattat tattcaaacc
      661 cgattgtgcc tgctcggaag aattactgat tctatcaata agatcacctg tgtgatagct
      721 atcatagttc ttcattaaag cccttgtaag ttttgaaatc aatgcggact gtaaattgcg
      781 tatagaaatg aaatttagaa tttccccaaa ataacctctg gtaaattcaa taacctgatc
      841 tgtgcctaat gctattaagc ttataataac gccattaaca agcagatttt tatcacctgt
      901 tgttgttcca ttaacaatac gacgcaatcc ttcagtatca agcaaacctg ttgcagacac
      961 tgcaatagca gctattaaaa gtcctatgta ccagggtata tattctttgc taataaaaat
     1021 aagtctttta agcgtttcta taggtgtcaa gcctgcaggt gtcagacgac tctcagccgc
     1081 agtttgcttt acagctttat tccgtttagt gaacattttt cggttattaa tttcagttct
     1141 gccctgcatt ataaatcccc cctgcacagc cacatgccgc taacattttc tcagcttcat
     1201 ctgcactgat tttaccgttt ttatataaat ccataatctt aactgcattg gtatttgcct
     1261 taatttcttt aattttcagc tcctcttttt ccacctgagt tttactctta gattccatat
     1321 gagttttacc ctcgggttct acctgagctt tattatttga ttcgatctgg atactattat
     1381 tcgagataat ttcacctatt tgacaggtat catcaattat tatttctcca tcgcaaatta
     1441 gtttaccaac gatacaatga tctttaagct ttacgctgcc tgcccttatg attttcgatt
     1501 ttaatccgct tgcggtaaga tctgtacatt ctaaaatatc cacaaggcag tgggacaggc
     1561 ttatttttct tccaaagtac aacatcatca aaaacataaa tttaataggg attttagaat
     1621 ccttccaact atttatattg ttggtaatag taattttgtc accatacatt tttgcaacgg
     1681 aacaagaccc gtcaatacat atttcatctg cagaaacttc acgggtggct acgatgatac
     1741 catcacaaac aattttgtcc gcatttaaat cagctcgtcg aagctttagt acaccattaa
     1801 tttcggtata ctttgattta atattacgaa agcatcttgc aacaccttca acaaccatct
     1861 gatccgtaat tagtctgcct tttcctttaa acagtccttc tactacgacg ctttttgccg
     1921 cagcatcacc ttttaattta ccaataccct ccaccgctat cgagtcatac tcaccaccat
     1981 agaaagaacc aattccttct attttttcac tgctcatatc tttaccacct cgtttaccac
     2041 tgctttttcc gatatacgaa cactatttct atattcaacc ttttctataa tacacaaatc
     2101 acctataaaa acatccattc ctgaaacaag ctctgatttt gtgttttcaa ttattatgtt
     2161 gtcgccttca ataacgttaa ctatgattat attcccgctc cctgccaatt taccgtaaga
     2221 tgcagacttt ggtatatttt taaatttttt atccggatta aaagtattac tgattcttat
     2281 gttgcttcca acaatacatt caagtgatat atcatcattt atcaaaataa atatgttttc
     2341 cgcatttaag gcttgagcac tgaggcctcc aaagctgtaa aagttttcac acacgatttc
     2401 attattacag tttagtatag ctgatgatat gatgttttta aattcataat cacagtttaa
     2461 agtaaagcta tataagcttt caaatgtttc tgcctgaagg ccgcccgacc attctaatac
     2521 tccatttatt tttgagtttc ttgaactatt cctaagaaca ttacattgga acaattctgc
     2581 attcaaactg cccgttataa cagcggtttc tgctttgcat attcctttca agtttacgtc
     2641 accagcagct ttaaagctga taaagtttgt acttgatgca tcaatatcac ctgcacagcg
     2701 aactttttca gcagaagaat ttttaatatc agtatcacct gcacagtaca gactttttat
     2761 tgtactgtcc ttgataataa tttccccggc agaaagaatt cttccaaaag aaccttgaat
     2821 acttctgttt ccaagaacaa ccatattcct tctcatatag tcaccccttt gggttcacaa
     2881 gcgctaataa atactgtgtt ctcgtcctct tcattatcaa atttaaaatt aaattttttc
     2941 cggtatttta attttaaacg gtttgaaatt tcatcaagat gaatagattt aacaatattc
     3001 aatctgatgt caaggtatac ttgtgcctgt tcagtgtaaa ttgttgcata atagtctttg
     3061 tttttatcaa ataaaacaaa gatatacccg gtagatttca tatttgaaag ataacctcgc
     3121 aagccttcag caatttcaat gatttgccct atagtaatat cgcagtcacg tttaaaatca
     3181 gatagtgcaa tcatgatcaa cacttctata taggtaaaat tatttttatc aaaccccttc
     3241 acaaaagcgg gaataagccc tttttgtatt tcctcaataa tatttaaatc atcagttgta
     3301 aaatttcgtt cggcaacttc cggtgaaagt atttttgcaa gctcttctaa agagtattta
     3361 tcctttagtt cctgtatagc tcttatacga tttagaattt tggttcttgg gaaaaaggtt
     3421 tcctgtccag tgaaagatgg ttttttaata aaccaatctt ccggaattaa cccttcccgt
     3481 ttccagcgat aaagctgacc atatgaaata ccggtttcat ttagtaaatc ttttttcgat
     3541 attagcatta tcagcgctcc cttcgtaaca ttgtttcatt gtctgatttc agtataacaa
     3601 aacattgtta cgttgacaag agtttttaga atttttttga atttttattt ctcctatgtt
     3661 ttttctccag aagtaaagat aaatgcgaag aagcattatc tttattatat ttattatctt
     3721 tattatcttt tttttcatta tattcgtttt tattatcatt atctatgttt tcatttatag
     3781 aggtattatc tatagtggta tttttgccct taatcttagg gtttcgtatg ttaacctctt
     3841 catgcacata ctgtacggta ctatcttttt gttccatact cccaggtttg gggaatgcat
     3901 ttttcacata tttatatttt atatgtcttt ttgtctcctg gattacatgt gctgcttgag
     3961 tcattctttc tgtagctgca tctaacttga ggttcagcct tcttatccct atctccagaa
     4021 caaatactat tatagataag gctataagga tatttgtcat gtctttgtta cctgcaaccg
     4081 gaggtggatc tcccttaaaa acctcagcac cgctgccgag gattcttcct cccccttcat
     4141 atgccaattt ctgcagaaag gctaagttat caaccctgga gatgttatac tcaggagaat
     4201 atggtatgga tattcctgta ttaatattct tcactatttc tgtaccatat ttaacattaa
     4261 tacttgcaag ataaattcca gtttcatcag cctggaaaat tccttcatat tttatacgag
     4321 atattggctg aagccggaat tgctgttcct ttcccgatgg acttacaatt acagcttcaa
     4381 ttacaggttc aattacaggt ccaataacag gtccagatgc tggtttagtt acttgtccaa
     4441 tcacttgtcc agtaacttgt ccagacactt gcccagatat agattctagt atagattcag
     4501 gcataggttc agatataggt tcatcattta tatttggtaa ttcaacttca attgctccta
     4561 ttccccctgc atctttgatc tgccctttat cagtacttct atcagtacta tagttttcat
     4621 atagtcctcc ctttatacta taatcatcaa tagaatgtct ttgtataatc catgataata
     4681 cattcttcca aaacaacggc gactgttccc atttcatcca gttccttgtc catgcacctt
     4741 ttacatcggg agtccaagct gcagtccttc ccagtccata ttgccacaca gccaggatgg
     4801 gctggtcggt atcacttgca aataccacct gggcagtatt ctttgctgta gtacccacat
     4861 atccttcaag aacaggtatt gaatcaatcc ctttaagtat ctctgattgg cttttcagtc
     4921 ctggtgcaaa aaccctgtta tttagataag ttttacctgc aaggaaggtt tcttttgcaa
     4981 aaattttagg tatatgtgaa aattcatctg tcatatagaa tctcccttta cctcctgctg
     5041 caagtgcatt taataactgc atatctgctg attgccccac agcaacggta gagagtgtaa
     5101 tccccgccgc atttatttta tcaatcaaag gctcatagcc tgtcttttca gcctcaccat
     5161 cagtgagtaa tattatatgt ttaagttttg tatcagcatt cttcaaagat aaatatgcct
     5221 cttcaagggg cggtaatatt tgtgttcctc cgccggctct aatggttcca atatcatcct
     5281 ggatttttgg gaggttatca agtttttgtg tttttacaac ccactgggct gcactatcaa
     5341 aggcaataac ccctatcata tctctgggag tcaaaacttc cgtagacctt atagctgctt
     5401 cttttgcaag ctccactttg gatacaccat attgtccccc agtcatactg ccggatttat
     5461 caataaccag cacaaggcct aaatttggtg attcttcttt aggagttata tccatgttta
     5521 ccggcaaaac tttttcaaga ggagtttcat aatatcctcc cagagcatag ctgttttctc
     5581 ctcccgttac cagcaagccc ttcccctggt gctttataca tatttcgaga ttattcagga
     5641 acctttcatc cagttcctca gctgaaacat cggatataat aaaagcctga tacataagca
     5701 tatcttccag tgacttagga acattttccg gttcaactgt cctaacctta acatcctttt
     5761 caaatattct tatcaattcg gaggcacttc ccagggcatt tccagcaaca tttccagatt
     5821 tattctgtat aaccagtatt tcaggaatat cctttatatg gctgaatgct gccatggaat
     5881 tattcttaat aaccccgtca tcttcgggtt caataactgc cgtataaact accagacccc
     5941 catttcccgc tgttcctgag aacacaaagg tattcattcc ttcatctatc tccacacttc
     6001 tttcagcagc taattgcctg tcagcatata atttaagaga ccccttggtt ttgacagtac
     6061 tgtcaacctt gactgtaact tcaaactttt ctcctttatg aagcacctca ggtatagtca
     6121 cctctttaat ctgtacttca ttcacttcct gggatttttg ctgttttaca tgcataacat
     6181 caacaactat acctttattg ttcagtaatc cggccagttt aaggctatct cccatatttt
     6241 cttcgccatc tgtaacaagt acaattcttt ttctgtcact gccgggtata agagaagatg
     6301 caaatcctag agcctgttca atattagtat aattcgcatt tatttttgat tgtattgaat
     6361 caaaaaaggg atttgctgtt ggtattgttt ctacagttgc attagcaccg aaaacaacta
     6421 taccggcctt ttcttggtgc tttttatttt tgaacgcatc ttttacaaag tcctcaattt
     6481 cagttttatg cctgtatgtg ctatctgaaa tgtccagagc aaatatggtt gtagtggaat
     6541 ttgaaacctg cctgatatta aagcctgaaa ttgcaaatat cagcagcaca aggacaataa
     6601 gtcgtatagc tccggttaat ttccttttgg aatttgaaag tcctcccatg ttgcgtaaaa
     6661 ggtatatggt taaagctgca gcaggtatta ttactaatag aataagagga tggttaaaag
     6721 aaaatatgtt aatatccatt tgtataaacc caccattcca cacataaaag taagagtaca
     6781 atccaaagca tgatatttct taaagagaaa cctgcagatt ggtttctggt tccacttgta
     6841 tccagtgtgc caagtgtacc acttgtttca cctgtttgaa tcgttttcgt ttcttcatct
     6901 gtttttgctg ctttatctga tgctgccata tcgtttattc cggtctgtcc tccaatccct
     6961 gtgcctgcat ctttcgcttc ccatgtttgt tccggctttt ccacttctct ctcaaatatg
     7021 tccccttcct gctctgccgg gacattaact gcaaaataac ttcggattct attatcttgt
     7081 tcaagaatgt acacaccggg ttcatgggta taatcaaaag ccttcaccgg aaatggagga
     7141 gcaattacaa ttgattctcc ggaaggtgtc agtaccagcg cttcctcagt tttaggatta
     7201 aggctgaatt ctatttccag gccagggtat atacttccta tactcttaac tgccggtggc
     7261 accagccact ctatagcatt cgtcatcatt atggggaatg cagacttaag taccatatcc
     7321 gtattatgaa ggtcaaatcc aaaaaccagc agccttcttt tatcaatctc tccagcaaaa
     7381 gcgatagtac ctttatcaga gcccagcact tcatctcccc atacaggcac atcaaataat
     7441 tttgatttat atatagagaa attataatca ttaatatgtt caaaaagctc atgatttgtt
     7501 ttttccgttt gtggaagtcc agcttcccct ttaacagtaa atatgtcatt tttaggcggg
     7561 ttaaaaatca gaatatttcc atctagaggc agttcatcca aagacagctt cccgctgtta
     7621 atacgccctg cgctgtctct acttcctgta ttgtctttac ttcctgtatt gtctttactt
     7681 cctgcactat cactgtctgt attaccaaaa atgttgtcaa atatataaag gtcgtaaccc
     7741 tttaattcct gtatatcctc aatactgcct ttatataggt caatattccc cattaatttt
     7801 aaaacctttt ccaggaacag attgcctttt gtaatcatga caactcttgc cttttctgaa
     7861 gaatttatta ctgtccaggc tgtgttgtca gctttcagag aatcgtctct gtcaatacgg
     7921 cactcaagaa ctctcgcatt atcaggtatt ctgctccaat atacattagc tctttctccg
     7981 gcttttatga caacttctct ggcatcagcc acctttccat ctgcatatag gctcataggc
     8041 aaagtttcat cgttttcacc aaaatttgct acacggacaa ggagacttat gctgttatcc
     8101 tttaaaagtg tatgagacat atgagtaata gcaaaattac ttccctcccc cataaccttc
     8161 acgaattcta tgttttgatt tccttcacca acaataaatt ctttatctcc aaaacatatc
     8221 acattaacat ccggatcaac ttcccaaagt gagtttataa tgcttgctgc ttcgctaaaa
     8281 ttagaaattc cattagtgac cttcaaagct ttaattttat cggcaactct ggctttgtca
     8341 ctactcatgt tctcttctat catagcttta cttcccatgc tgataagagt tatctgtgtt
     8401 tcaggttcaa gcctggtcag gtagtctccg gccagttttc tggcagcctc aaatctgctg
     8461 ggcttaacat caacagcctg catactcatg gaggtatcta ttaatatggc aactttttta
     8521 acttttttgc ctcctgcgtt tataaatgga ttggccagtg caaaaaccag aaaaagagct
     8581 gcagtaattt caagtatcat aagaagattt ttctttaatt tctgccaggg tgtacttgct
     8641 tcaacatctt ttaaaacttc ctgccagaga taaagactcg aaacagtatg ttctctgtac
     8701 tgctgcttca aaagataaaa caggattatt accggtattg aaattaaata tataaatgca
     8761 taaggtgaat aaaatttcat ttatcaatcc tgcctctcaa cttcctaaca tcatccattt
     8821 tttgttattt cctctatgtt tagtactttt taattatttt cactgtattt tacacttata
     8881 ttgctgctat cacacaccta tgaccttact ttattcaaac ctgtaatcat acttatcatt
     8941 tgttctatag gcattgcagt atccatcata tagtaggctg ctccttttct aaagcattct
     9001 tccctgatat tggcaataaa gtcattaaat attctgttat acgattttaa gagtctgggg
     9061 gtagcagtaa catcaatagt ccgccctgtt tccgaatcta ctagtctcat gtttgtaccg
     9121 atatccggtc tggcttccca tggcgcaagt atctgacaaa tatatacatc ctgttttcta
     9181 tactgcaggt actttatcat ttctgttaac cctcctggtg aaaacagatc ggaaattatt
     9241 attgaaatac ccctgttgtc ttttaaatta taagccttta ttgcctcata aaaacccgga
     9301 ccatttttgt attcaacctc ttcaagaaag gttagaagtt ccggcaggaa atgctttcct
     9361 cttaaagata atctggattt ttcaaccctg tcacttaaac aaataactga aactctgtca
     9421 taagcactta aactcatata tcccaatgcg gcagcaagtc tccttgaagc aaaactctta
     9481 ttaggctctc cccaatccat ggacttgctt gtatcaagaa atatgttaac aggggcttcc
     9541 ctttcctcca taaaaagctt tacatatagc ttttcaaacc tcccataggc attccagtca
     9601 attcttctaa aatcgtctcc ctctgagtac tccctgtaat cagaaaactc cactgaagat
     9661 cctttttgcc tggatttcct atttcctccc gctcctcctg aaactataat tcctgttgat
     9721 aaggtcaact tttgtatttt gtttaaaaaa tcgctatcaa aaagctcact catgactttt
     9781 caatttcctc aattatagtt ttaataaggt catcagtagt caaaccttct gaaatacctt
     9841 caaagtttaa aaaaattctg tgccttaaag caggatatgc agcatacctt atatcatcga
     9901 aagctacatt atatcttccg tcaagtattg cttttatacg tgcagtcgac actacagcct
     9961 gagctcctct gggacttgac ccgaagcgga tgtatt
//
LOCUS       DAOSDD010000170         9986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2557_length_9986_cov_5.060618, whole genome shotgun sequence.
ACCESSION   DAOSDD010000170 DAOSDD010000000
VERSION     DAOSDD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2557_length_9986_cov_5.060618"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK733_13730"
     CDS             complement(<1..516)
                     /locus_tag="PK733_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ11637.1"
                     /translation="MKRKILIVDDEIDMLDMLRSFFELKDYEVFTALDGKEVMEKLNE
                     KPDLILLDVNMPQLDGFKTCQMIRDKTSIPILFLTARTGEQDRVKGLMIGGDDYIIKP
                     FSLKELAARIHAHLMRENRRNRRNDASAFGELWIDYSGRCIFYRNKEVILTKTEFDII
                     EFLSLNSGRVFD"
     gene            complement(954..1259)
                     /locus_tag="PK733_13735"
     CDS             complement(954..1259)
                     /locus_tag="PK733_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11638.1"
                     /translation="MLAGYRGKYRSIGTVIDFGNSNFTPFYMNEGGICKYGGWDLDAL
                     DICKEYSISKIEYDTNNLVFNFEGTLFAAMLQLKIGKDPRPHIDRFLKNCRHYMQIN"
     gene            complement(1748..2890)
                     /locus_tag="PK733_13740"
     CDS             complement(1748..2890)
                     /locus_tag="PK733_13740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ11639.1"
                     /translation="MNKNKNESQITVDKKPGKEPGKKISLYVLLGFSVLIVLVAAIAG
                     FSSYSRTYVASVGNMKISIPEYRFVLNQEKANMLKIAGNPDPATFWDTVIADGEKAID
                     IAKRKSLENLRDLKIQVSKAKEQKIVLDKTDLDNVNQFTQSIIAQNNNNKSAANSFTM
                     EVYGVNLKEFEEIYKQYILRSKFVQNEMKSIDAKEEEIETYYDKFPDAFKDASYRENG
                     QEAVWAKHILVLTIDTETQKEFTGAKLEAATKKAEELLQRAKGGEDFAKLARENSEDT
                     GSAETGGDYVFGKGYMDPEFEKTGFELEPGQISGLVKSSYGYHIIKLEEKIPQGEPVS
                     LRCAKEYMEFKENAVRIAKYMEKLEEWKKDSKYKVSRNNKVYDTIE"
     gene            complement(2959..6609)
                     /gene="mfd"
                     /locus_tag="PK733_13745"
     CDS             complement(2959..6609)
                     /gene="mfd"
                     /locus_tag="PK733_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ11640.1"
                     /translation="MAVNMNNLDYLLEPLLEMEEYKSILEQLNRINLDAPFNIAGPSD
                     SQKAHLAYAICRHLNRKGIFITFNEMQARRFYEDFSFFLGDKVVMFPTKETLYYDVEA
                     KSNDAVYKRITALARIIEGDYSIIVTSVDAVSNKLVPEKLFSSYILNIENGSQLDIQH
                     LINNLVPMGYERADIVEGKGQFAVRGGIIDIFTVDQEDPLRIELFGDEIDSIRTFDVL
                     SQRSIAKLDKVKILPAREIICPVSRREGIIKQIKRDLGSYVNTLKGKKGTDFIKQVRT
                     SIEYDIERFENSFYFPGIDRYTSYIVDQPCHLADYVKDNPGGHPGDILGSYHGSCYGD
                     HSDGNLIVFMDEPVRLSQRMDNLMLENHEVCKNLLEKGRILPQNTDILYDYENILRGL
                     KTKQKVYLSAILHDGQQDFRQDSPRDFSQDLQHNILCKQNPSYYGFSELLVNDINSMK
                     NNGSRIIILAGTKTRGEKLRDNLVSDGIEAVYNDAINDSLQPGKVTITHGSLNKGFQY
                     PSIGFVVISEREVFGQDRRSRKTPKKKAKGAQINLFTDLNPGDYIVHQTHGIGQYVGI
                     EKLVVENIHKDYLKIRYQEGDFLYIPTNQLDTVQKYIGAEGKAPKLSKLGGNDWTKTK
                     KRVKESLKELAEDLVKLYAERKAIKGYSYSKDTVWQNEFEELFPYEETEDQLKCIEEI
                     KNDMQSGKPMDRLLCGDVGYGKTEVALRAVFKAIMDGKQVAFLVPTTVLAQQHYSNFI
                     ERMKKFPVTVEMISRFRTRADQGKILKDLKKGHVDVLIGTHRLIQKDIQFKDLGLLVI
                     DEEQRFGVMHKESLKNARPDVDVLTLTATPIPRTLHMSLIGVRDISVIEEPPEERYPV
                     QTYVMEYNDDVIKDAITRELARNGQVFYLYNRVRTIDIKAFQIQELVPEARVGVAHGQ
                     MEERKLEDVMLKFINGEFDVLVCTTIIESGLDMPNVNTIIVEDADRMGLAQLYQLRGR
                     VGRSNRLAYAYITYKKDKVLTEIAEKRLQTIKEFTEFGSGFKIAMRDLELRGAGNLLG
                     PEQHGHIESVGYDMYCRLLDEAIRELKGELPVEREIEVTIDLNVNAYIDDEYINRELQ
                     KIEMYKKIASIRDEQDVIDIRDELLDRYGDLPAPVENLVDIAYIKALSGELGITSITE
                     KDGAVILQLRNDKSISFEIIGKLAAKYKRQILFNAGNNPYLAYKMAENSREKFLENIK
                     ILLHEIKSFEVS"
     gene            complement(6647..7228)
                     /gene="pth"
                     /locus_tag="PK733_13750"
     CDS             complement(6647..7228)
                     /gene="pth"
                     /locus_tag="PK733_13750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ11641.1"
                     /translation="MGSLENLYIIAGLGNPGIKYKDTRHNVGFLVIDALSEKYSINVM
                     QLKHKAIVGSGAIDGTRVLLVKPQTYMNNSGESIRDIVEWYKVPLEHIILIYDDVDLP
                     TGKLRIRPKGSSGTHNGMKSVIYHLQSDNFPRIRVGIGSTPEGWDIADYVLGRFSEEE
                     KKLIFEGVVKAAEAVKVIITSGTDIAMREFNGL"
     gene            complement(7502..8455)
                     /locus_tag="PK733_13755"
     CDS             complement(7502..8455)
                     /locus_tag="PK733_13755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOJ11642.1"
                     /translation="MNIHGKDIKIFSGNSHRGLAEEIAEKIGLPVGVATVGKFSDGET
                     EINIGEVVRGSDIFVIQSTCYPVNDNLMELLIMIDALKRASAGRITAVMPYFGYARQD
                     RKAKARDPISAKLVANLITVAGADRVLTMDLHVPQIQGFFDIPVDHLLGVPILAEYFK
                     EKFPDADDLIIVSPDVGSVSRARKLAERLESPIAIIDKRRPKANVSEVMNIIGDVSGR
                     RVILIDDLIDTAGTIVNAAEALSNAGAKEVYACGTHGVLSGPAVERIKQSAIKELVVL
                     NTIPLTGEKRIDKIISISVAPIFAEAIERIYGDISISSLFT"
     gene            complement(8536..9915)
                     /gene="glmU"
                     /locus_tag="PK733_13760"
     CDS             complement(8536..9915)
                     /gene="glmU"
                     /locus_tag="PK733_13760"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOJ11643.1"
                     /translation="MENLMALVLAAGEGKRMKSKYSKVVHKACGKPIIKWVCGTSKEA
                     GIDETVIVVGHQADQVMECLGDGYQYVWQKEQLGTGHAVMQAEDILKQKEGLVFILYG
                     DTPLITADTINKAVAYHKNQNYIATVVTAEVDDPSGYGRIIRNSQGDVQRIVEHRDAS
                     PDELKVREINSGMYCFSVKHLIAALRELKNDNDQGEYYLTDTLEILIKKGFKVGAYVV
                     ENPVEILGVNDRVQLYQVSEFLGKRILEKHMKEGVTIIDPGNIYIDDSVKIGVDTVIY
                     PGAILEDGSVIGEDCIIGPNSRIISSHIGDRVQISSSVIIESSVGNDACVGPFAYIRP
                     ESRIGQNVKIGDFVEIKKSVIGGKTKIPHLAYIGDAEVGDNTNIGCGVITVNYNGKEK
                     NKTIIGNNAFVGCNVNLVAPVEVKDNAYIAAGSTITEEVPEYSLAIARERQVIKDNWV
                     IKKGMKRDD"
BASE COUNT         2905 a         2247 c         1410 g         3424 t
ORIGIN      
        1 atcaaacacc cttccggaat tcaaggataa aaactcaata atatcaaatt ccgttttggt
       61 gagaatcact tctttgtttc tataaaaaat gcaccttccg ctataatcaa tccacagttc
      121 accaaaagct gaggcatcat ttctcctgtt tcttctgttc tccctcatca aatgggcatg
      181 aattcttgcc gctaattctt ttaaagaaaa aggtttgata atataatcgt ctcctccaat
      241 catcaaacct tttaccctgt cctgctcacc agtacgagcc gttagaaaaa gaattggtat
      301 actggtttta tctcttatca tttggcatgt tttaaaacca tccaattgag gcatattgac
      361 atcaagaaga attaagtcag gcttttcatt taatttttcc ataacttctt ttccatccaa
      421 agctgtaaac acttcatagt ctttcaattc aaaaaaacta cgcaacatat ctaacatatc
      481 tatttcatca tcaacaatta aaattttccg tttcatacta tcacctaata atatagcata
      541 ccatataatt ctctattttt tatcaacttt tttcatatat aactatatag aacaggttta
      601 aacatttaaa acctgcttct gttaccttga tgtaacttac gatttgcaaa ttcttcatta
      661 gttctttcaa atcgcaaatt atatagatag aaattttaac tttaaatgcc gtacatttaa
      721 tggaaaccct gtataacatt acacaaaggg catggagaac gcctcgtcac gatctttgcc
      781 gtcaagcagc ggttttacaa tatcaagtat cttcggcgat gctgaaacga cgatggactt
      841 gcccatagta tataaacaat tcggattatg ataagaaatg caaatataat gagcggcaaa
      901 taaaagtttt agatgaattt gaggtagcaa aatatatttt atagtagtga attttaattt
      961 atttgcatgt aatgcctgca gttttttaaa aacctgtcta tatgaggtct tggatctttg
     1021 cctattttca attgaagcat agctgcaaat aaagtgcctt caaaattgaa aactaaatta
     1081 tttgtatcat actcaatctt actaatagaa tattctttac atatgtctaa agcatcaaga
     1141 tcccatcctc catatttaca tataccacct tcattcatat aaaaaggtgt aaaatttgaa
     1201 ttcccaaaat caataactgt tcctatgctt ctgtatttac ctctatagcc tgccagcaca
     1261 ttatgttttc ccaggtcccc atgattaaaa gacgcaaaat aagaagacct aatatgtttt
     1321 agagcagttt ctaatacact ggaaactata tgcaattctt cgagtcgaaa aatgactagt
     1381 gtaacagcat cttgctacga aaatagaatt taaaattgct taaaacactt atgaatactg
     1441 aactttgaca acttaacata attgcaataa catgcaacct attttcattc gaggttgtga
     1501 gagccaaatc atgagtcgtt taaaatagat aatggctaaa attaacatag taagttgtgt
     1561 ataaaaaagg aacataaata tacaatactt tccccacgaa aggagtacct caatgtatat
     1621 ttacatccct ctggaattaa tctagatatt gttatgcaca aaagaaagtg aatattatac
     1681 acagaaatcg atttatgcca tatttttctt caaatataga aaatgcgtac catagccaca
     1741 ttcatactca ttcaattgta tcatacacct tattatttct tgatacttta tatttagaat
     1801 cctttttcca ctcttccagt ttttccatat attttgcgat tcttaccgca ttctctttaa
     1861 actccatgta ctccttggca caccttaaac taacaggttc gccttgaggt attttttctt
     1921 caagtttaat aatatgatag ccataacttg atttaaccag cccgcttatc tgacctggtt
     1981 ccagctcaaa gccggttttt tcaaattcag gatccatata ccctttacca aaaacataat
     2041 caccgcctgt ctccgcagaa cctgtatctt ctgagttctc acgggccagt ttggcaaagt
     2101 cttcgccgcc tttagctctt tgaagcaatt cctcagcttt ttttgttgca gcttcaagtt
     2161 tggcacctgt aaattctttt tgggtttcag tatctattgt aagcaccaat atatgtttgg
     2221 cccacacagc ttcctgtccg ttttcccgat aagacgcatc tttgaaagca tctggaaatt
     2281 tgtcatagta tgtttctatc tcttcttctt ttgcatcaat tgacttcatc tcattttgta
     2341 caaatttgct tcttagtata tattgtttgt atatctcttc aaattccttt aaatttactc
     2401 catacacttc cattgtaaat gaattagctg cagatttatt gttattattc tgtgcaatta
     2461 tggattgtgt aaattgattg acattgtcca aatccgtttt atcaagtaca attttttgct
     2521 ctttagcttt ggatacctgt attttcaaat ctctcaaatt ttcaagagat tttcttttgg
     2581 caatatctat ggccttttct ccatcagcta ttactgtatc ccaaaaggtt gctgggtcgg
     2641 ggtttcctgc tattttaagc atattggcct tttcttgatt caaaacaaac ctgtattccg
     2701 gaatgcttat cttcatattt cctactgaag caacataagt tctggaataa gatgaaaacc
     2761 ctgctattgc agctactaga acgattaata ccgaaaaacc caacagcaca tataaagata
     2821 tctttttccc aggttccttt ccaggctttt tgtcgacagt tatttgactc tcatttttgt
     2881 ttttattcaa ctgaatctct cctctattta tcaataaatt taagttaaag aatattaatt
     2941 ttattataca acatataatt agctaacttc aaagcttttg atttcatgta ataaaatctt
     3001 aatattctcc aggaatttct ctctgctgtt ttctgccatt ttatacgcta aatatgggtt
     3061 gtttcctgca ttaaacaaaa tttgcctttt atattttgca gcaagcttcc ctattatttc
     3121 aaaacttata cttttatcat tccttaattg taaaataact gctccatctt tttctgttat
     3181 tgatgttata cccaattctc ctgataaagc ctttatatag gctatatcta ccagattctc
     3241 aacaggtgca ggcaagtctc cgtagcggtc aagcagttca tctcttatat caataacatc
     3301 ctgttcatca cgtatagaag caattttctt atacatttca atcttctgca attccctgtt
     3361 tatatactca tcatctatat atgcatttac attaagatct atagtaactt ctatttccct
     3421 ttctacaggc aattcgccct taagttccct tatagcctca tcaagcagac ggcaatacat
     3481 atcatatcct acggattcta tatgcccatg ctgttcgggg ccaagaagat ttcccgcacc
     3541 cctcagttca agatctctca tggcaatttt gaaacccgag ccgaactctg taaattcctt
     3601 tattgtttgg agccttttct cagcaatttc agtcaggacc ttatcttttt tataagttat
     3661 atatgcatat gccagcctat tggagcggcc aaccctgcct ctcagctggt ataactgggc
     3721 caggcccatc ctgtcggcat cctcaacaat tatagtattt acattgggca tatcaaggcc
     3781 tgattctatt attgtagtac atacaagtac atcaaactct ccatttatga acttaagcat
     3841 tacatcttcc agttttcttt cttccatctg tccatgagcc acaccaactc ttgcttcagg
     3901 taccagttcc tgaatctgga aagctttaat atctatagtc ctcacacggt tatataaata
     3961 gaacacctga ccatttcttg caagttctct ggttattgca tcctttatta catcatcatt
     4021 gtattccatt acataagttt gcacaggata tcgttcttcc ggcggctcct ctataacact
     4081 aatatcccta acacctatga gcgacatatg gagggttctt ggtatgggcg tagccgtaag
     4141 tgtaagaaca tcaacatccg gacgtgcatt tttaaggctt tctttatgca taacaccaaa
     4201 acgctgctcc tcatcaataa ccaataaccc caaatcctta aattgtatat ccttctggat
     4261 caatctatga gttcctatca atacatcaac atggcccttt ttaaggtctt tgagaatttt
     4321 accctgatcg gctcttgtcc tgaatctgct gatcatttct actgtaacag gaaatttttt
     4381 cattctctca ataaagttac tataatgctg ctgggctaaa actgttgtag gtacaagaaa
     4441 agctacctgt ttcccgtcca taatagcttt gaaaaccgct ctcaatgcca cttcagtttt
     4501 tccatatcct acatccccgc acaaaagcct gtccatgggc ttgccggatt gcatatcatt
     4561 tttaatttct tcgatgcatt tcaactggtc ttccgtctct tcatacggaa acagttcttc
     4621 aaattcgttc tgccatacgg tatcttttga ataggaatac cctttaatag ccttgcgctc
     4681 ggcatacagc ttgaccaaat cttctgccag ctcttttaga gactctttaa cccttttctt
     4741 ggtttttgtc cagtcatttc caccaagttt gcttaattta ggagcttttc cttcagctcc
     4801 aatatatttt tgtactgtat ccagctgatt tgtaggaatg taaagaaagt ccccctcctg
     4861 gtatcttatc tttaaataat ccttgtgaat attctctaca accagttttt ctattcctac
     4921 atattgccct attccatggg tttgatgaac tatataatca ccggggttta agtcggtgaa
     4981 aagattaatc tgggctcctt ttgccttctt tttaggagtc tttctgcttc ttctgtcctg
     5041 accaaaaact tccctttcac tgataaccac aaatcctatg gaaggatact ggaagccttt
     5101 atttaaactt ccatgggtta ttgttacctt cccgggttga agagaatcat ttatagcatc
     5161 attatataca gcctctatcc catcagaaac aaggttatcc ctcagctttt cacccctggt
     5221 ttttgttcct gcaagtataa taattctgct gccattattc ttcatggaat ttatatcatt
     5281 gaccagaagt tcggaaaacc cataatatga aggattctgc ttacacagaa tattgtgctg
     5341 caaatcctgt gaaaaatccc gtggagaatc ctgccgaaaa tcctgttgtc cgtcatggag
     5401 aatagcactt aggtatactt tctgctttgt cttcaagccc cttaaaatat tttcgtaatc
     5461 atacagtata tcagtatttt gtggtaatat acgccccttt tccaataaat tcttgcatac
     5521 ttcatgattc tcaagcatta aattatccat tctttgacta agtcttacag gctcatccat
     5581 gaagacaata agattgccat cggaatgatc tccatagcaa cttccgtgat aacttccaag
     5641 aatatctcca ggatggcctc caggattgtc tttaacatag tctgccaaat gacagggttg
     5701 atctacaatg tacgaagtat agcggtctat tcccgggaaa taaaagctgt tttcaaacct
     5761 ttcaatatca tactcaatac ttgtcctgac ctgctttata aaatctgtcc ccttcttacc
     5821 cttcagtgta tttacataac ttcccagatc ccgttttatt tgttttatga tcccttctct
     5881 tctggaaacc ggacaaataa tttcccttgc aggtaaaatt ttcaccttat caagtttagc
     5941 aatagaacgc tgtgacaaaa catcaaaagt ccttattgag tctatttcat ctccaaatag
     6001 ttcaattcgt aaagggtcct cctggtctac ggtaaatatg tctattattc cgcctcttac
     6061 ggcaaactga ccttttccct ctactatatc agccctctca taacccatgg gtaccaggtt
     6121 atttatcaag tgctgaatat ccaattgaga gccattctca atattcaata tgtagcttga
     6181 gaacagtttt tcaggcacaa gtttattaga aacagcatcc actgaagtaa caataatact
     6241 gtagtcacct tcaattatcc tggcaagagc cgttatgcgt ttataaacag catcattgct
     6301 ttttgcttca acatcgtagt aaagggtttc ctttgtggga aacattacta ccttatcacc
     6361 aaggaaaaag ctgaaatctt cataaaagcg tcttgcctgc atctcattaa aagtaataaa
     6421 aatacctttt ctgttaagat gcctgcaaat agcataagcc aggtgtgcct tctgagaatc
     6481 ggaaggccct gctatgttaa atggagcatc taaattaatt ctattcagtt gttctaaaat
     6541 acttttatac tcttccattt ccaggagagg ttccagcaaa taatctaaat tattcatatt
     6601 aaccgccatt ccgctgctgt gcatagatac aaataataca ataatattat aatccgttaa
     6661 attctctcat tgcaatatca gtgcctgatg taataataac ttttacagcc tctgctgctt
     6721 ttacaacacc ttcaaaaata agcttctttt cctcctcgga aaaccttccc aatacataat
     6781 ctgcaatatc ccagccctcc ggggtactgc caattcctac tcttattctg gggaaattat
     6841 cggattgaag gtggtaaatt accgatttca ttccattgtg tgtgcctgaa ctccctttag
     6901 gccgtattct aagtttgccg gttggaaggt ctacatcatc gtatatcaat attatatgct
     6961 ctaaggggac tttgtaccat tcaactatat ctcttatact ttcaccgctg ttattcatat
     7021 aagtttgagg ttttaccagc aaaaccctgg ttccatcaat agctccactg ccaacaatag
     7081 ctttatgttt taactgcata acatttatgc tgtatttttc cgataatgca tcgataacca
     7141 aaaatcccac attatgcctg gtatccttat atttgattcc agggtttccc aaacccgcaa
     7201 ttatatatag attttctaaa cttcccaaaa taatgcccct ttcagtatac atataatgta
     7261 taaaggttga aaacaaaagc ttccttattt gggaagctca ttaatgtcta caatatctat
     7321 caagttacta atacataagc aacaaatcgt caagcagcta atgttcaaac aacctgatac
     7381 ataaacaacc aatgcttaaa caacctgatg cacaagaagc taatgtttaa acatgttgat
     7441 acacaagaag ccaattctgc agcatcccgt acactggttt ataatgacct tctgcctgcc
     7501 ttcaggtaaa taaggaactt atggatatat ccccgtaaat cctttctatt gcctccgcaa
     7561 atattggagc tacagaaatt gatattatct tatctattct tttttcccct gtcaagggaa
     7621 ttgtattaag cacaacaagc tctttgattg ctgattgttt tattctctct actgcgggtc
     7681 ctgataaaac tccatgtgta ccgcaagcat aaacttcttt tgctccagca tttgacaatg
     7741 cctctgcagc gtttacaata gtccctgccg tatcaatcaa gtcgtctata agtatgaccc
     7801 gccgtcctga tacatcacca attatattca ttacctcact tacattggcc tttggccttc
     7861 tcttatcaat aatcgcaata ggtgattcaa gtctttcggc cagctttctt gcccttgaaa
     7921 cacttcccac atcaggagat acaataatca aatcatctgc atccggaaac ttttccttga
     7981 agtattcagc caatattgga actccaagaa gatgatccac aggaatatca aaaaatccct
     8041 gtatctgtgg gacatgaaga tccattgtta acactctgtc agctcctgca acagtgataa
     8101 gatttgctac caatttggct gaaataggat ccctggcctt tgcttttcta tcttgccgtg
     8161 catatccaaa ataaggcatc accgcagtaa ttctaccagc tgatgctctt tttaaggcat
     8221 caatcattat aagaagctcc ataaggttat catttacagg ataacaggta gattgaatta
     8281 caaatatatc ggatcccctt acaacctctc ctatattgat ttcagtctct ccatcactaa
     8341 atttaccaac agttgcaact cctacaggaa gaccaatctt ctctgcaatt tcctctgcaa
     8401 ggcccctgtg ggaattcccc gagaaaatct ttatatcctt cccatgaata ttcaattcat
     8461 ataccttcct tcctaaaata atgctaccat tctaatgttg ccattctaat gctaccattc
     8521 agggcgataa gataatcagt catctctctt cattcccttc tttataaccc agttatcttt
     8581 aattacctgc ctttctcttg caatggcaag agaatattca ggaacctctt cggtaattgt
     8641 agatcccgca gcaatatatg cgttatcctt cacttcaaca ggtgcaacaa gatttacatt
     8701 acatcctaca aaagcattat tgccaataat tgttttattt ttctctttac cgttataatt
     8761 aactgtaata acaccacagc ctatatttgt attgtcccct acctctgcat ctcctatata
     8821 tgctagatgc ggaattttag tcttgccacc tataacagat ttttttattt ccacaaaatc
     8881 tccaatttta acattctgcc ctattctgct ttccgggcgt atatatgcga aaggtcccac
     8941 acaagcatca tttcctacac tgctttcaat aataacagaa cttgaaatct gcactctgtc
     9001 acctatatga gagcttatta ttctgctatt tgggccaatt atacagtctt cacctattac
     9061 agagccatct tccagtatag ctccaggata gatgacagta tccactccaa ttttaacgct
     9121 atcatcaata taaatgtttc cggggtctat tattgttact ccttctttca tgtgtttttc
     9181 aaggattctt tttcctaaaa actcagatac ctgatataat tgaactctat cattaactcc
     9241 gagaatctct acaggattct ccaccacata agcccctact ttaaatcctt tttttataag
     9301 tatctcaaga gtatcagtta aataatactc accttgatca ttatcatttt taagttccct
     9361 taaagctgct atcaggtgct tcacagaaaa acaatacatt cccgagttaa tctctctaac
     9421 cttcagttcg tcaggagaag catccctgtg ctcaacaatt ctttgtacat ctccctgtga
     9481 atttcttatt attcttccat aacccgatgg gtcatcaacc tccgctgtga ctactgttgc
     9541 tatgtaattt tggtttttat gatatgcaac agccttattt atggtgtctg cagtgattaa
     9601 aggtgtatca ccatacaaaa taaaaacaag tccttccttt tgttttaata tatcctcagc
     9661 ctgcattacc gcatgccctg taccaagctg ctctttctgc cagacatatt gatagccatc
     9721 tcccagacac tccataacct gatcagcctg atggcctaca acaattactg tttcatcaat
     9781 ccccgcttct ttagaagtac cacataccca ttttataata ggcttgccgc aagctttatg
     9841 aacaacttta gaatatttgg atttcatcct cttaccctct ccggctgcaa gcaccaatgc
     9901 cattaaattt tccatattaa gcgcctcccg tttaaaactc gtggtcacgg ggctaaaagc
     9961 cccgaaacta cggtattttt tgttag
//
LOCUS       DAOSDD010000171         9910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2578_length_9910_cov_4.618265, whole genome shotgun sequence.
ACCESSION   DAOSDD010000171 DAOSDD010000000
VERSION     DAOSDD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2578_length_9910_cov_4.618265"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PK733_13765"
     CDS             complement(<1..859)
                     /locus_tag="PK733_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11644.1"
                     /translation="MKQKFFKVVSLVMALVMCAGLFASCTVKKTNDGNNETKLTTPTQ
                     EKTTEVSSTPKTPAEILASGDLIKPDPSIKFPISEKPIKLRFMKPYVMYDTEYGELAI
                     LKEYAKKTNIEIEWDIPPQANFKEKYTLAMNTGNLPDAVIAPLDVEKYGQQGAFIDLK
                     SVIDSDMPNLKKAFAKYPFSKKVVTTDEGKVYSLPYIYQFVSGNNVMLLRNDLLKKHN
                     IAMPVTTDDWYNAMKTLKEKEGITPFSGYGESGKGMNAAISMIAAWGVFPEGLYVAEK
                     LAPQDNKVHY"
     gene            complement(1098..2000)
                     /locus_tag="PK733_13770"
     CDS             complement(1098..2000)
                     /locus_tag="PK733_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11645.1"
                     /translation="MTKLGKAIKESIGERIFYHIIILLLIITCIVVLYPLIHVVSVSF
                     SSPFAVMANEVGLLPKGFTIEGYRRVFINQDIWRSYLNTIAYTVVGTFINIILTSMAA
                     YPLSRKDFYGRKVWTLYFTFTMFFGGGLIPTYLLIQKLHLIDTFWVMVILGAVSTWNM
                     FIMRTFFTANVPFSLQEAAIIDGANDLKIFVSIVLPLSAPIIAVMTLFYGVGHWNAFF
                     NALIYLNNRAKYPLQLILREILLQNLLNARMAEGLGSAAEQEFIGESIRYASIVVATV
                     PILMVYPFLQKYFVKGILIGAIKG"
     gene            complement(2005..3006)
                     /locus_tag="PK733_13775"
     CDS             complement(2005..3006)
                     /locus_tag="PK733_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11646.1"
                     /translation="METLGAKMISPKLSMKNKDTSEKRGISSKKTGISAVACGFYRSR
                     YLYLLILPIIVYYFIFHYMPMYGVLIAFKDFNAFKGIISSPWVGLKHFKSFFESPFAY
                     RLLRNTLAINIYELVVGFPMPIILALLINEVKSNRFKRSVQTIVYMPHFISVVVICGM
                     LVSFLSPSTGFINRFIQAIGGQPIHFLAEPKWFRTVYVLSGVWQSAGWGSIIYLAALA
                     GIDIELYEAATVDGATKWQKMKHITFPGILPTVIIMLILRMGSMFSVGFEKIMLLYSP
                     LTYDTADVISTYVYRKGILDANYSYTAAIGLFNSIINFILLIAVNKFSRAVSETSLW"
     gene            complement(3248..5599)
                     /locus_tag="PK733_13780"
     CDS             complement(3248..5599)
                     /locus_tag="PK733_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11647.1"
                     /translation="MQSAARVLEQTRDITDNNLESFANISSNLSSNPHISYLLSHDNV
                     EKVSIYPYLSNAIKELNKYKNANSSIDNIFIFFKDADMVLSDTSRYYLESFNKYVLSW
                     QEMSTDEFKRILYNTHTQTILPSQTAIFNNLTRRIISYIQPIPMKDPFPKALLMITVD
                     ESSIKRSIETSLSQYEGYACVLDANNKIIMGNKSGNFLFDDGKRDALLSGLKDQEADS
                     CYITKYGFVVSSVKSQENNWKYISIIPSYTILDKVSDIQNRMTIILVSTLFLGVAIAL
                     YFVQNGYNNINTIIKAIRGFEHDTTLNGYKSEWDLINNTIVSYISKNESLQKKIYEQI
                     PIMKNTFFSRLLKSSFTDEQSVDEMLEFLSLNIKKDRYGVFILDVDSSNNVNSSEKNE
                     PIREVVQATLINIVENLVDKDCFVYALQDEAYRISVIIGVDDRSNNHSLGNDSSLNDI
                     SYDSRLDKRRLDNSIPNNISNISNISNTSNTNNISNRGLNNFEHDNYVYLVNIARKIK
                     DVVKNDLGFTVTIGIGGLYNKLWLLSDSYNEACEALEYKIIMGRDAVISYDAICSRGT
                     RKVCYSFKQEKELINFLKMGEYEEIQKILDEIVHTIKSEPVTIDFIKYIYFDIINTAM
                     KAADEINIKSSNIEIFINNLNNMGTLDEIYSAVSNFYLKLCDEVKDAKMSKNLELRDK
                     VIEYINKNYSDSMLSVEGIADYFSVSPSYLSRFMKDHIGYSITDYIHQVRLQKAKELL
                     QNTEKTVAVIAEEVGYNSLHNFSRVFKRYESITPTNFRTIGNL"
     gene            complement(5964..>9910)
                     /locus_tag="PK733_13785"
     CDS             complement(5964..>9910)
                     /locus_tag="PK733_13785"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11648.1"
                     /translation="WLRQLLEVANVLDGYEGYPGADLYKNPKFMKMFSAFYPLILAER
                     YTAQIGDSGGTGNPGLMVSISESVKGFEKFGDPVLAQLAYFLNNNSAGGLHGDIFTQD
                     PESIGPAIENVIAEKGKLDLGSTNLAGYGFSVLRDGKNNKQYFGIQIPFANLSAFDAT
                     IGFKHFASNGTIQFESDKIGDKISFRFNVEKTDIYEVDLNPFRAGSYGIYDISIDGTK
                     VKTLDFYGGSGADSNLEPVATLELTAGEHVITFEGVDKNENATGYKMGVIHIVLFDNE
                     SLKVKNDTTLLNTLRDFWMYYGRTNTSHAHRDQLNLGVHAFGLDISPDLGYPEETGDQ
                     PNRVEWVSNTVSHNTVVVDKTKQNDYYGGAPIHFDDSDTVDVMDVDSTGAYSQTQMYR
                     RTVAMVKVDDEISYGVDFFRVKGGSDHYYSFHGAEGAVATEGLNLIGQKDPEGNFVGT
                     YAGVNTQFGVRPSDDSVAGWSYKGAGFHWLKNVEKDSAPGNNFSIDWDVVDGRKVLPQ
                     EMDIHLRLTMLGKYDDVAIMDGVPPKKPGNPASLKYMIAHRSGQNLSSTFTAVIEPYK
                     DARYIESICEVPVTVNGEAAAGDVKAVKVVLKNGRTDYIVNSLDKETVYTVDGKFQFK
                     GFLGVYSEKEGQQVYGYVNDGTIIGTFNYDLPATVAGTVKSFTKEMSVNNELTVELAD
                     IVDLNEITGRYIYITNDGVQNAAYKVNGIKSVNGNEVTLDLGASTLIRSYVNSSDISQ
                     GFNYNIAEGNSFVIPMSLEKSKPPVDPGSGSGSDSDSDSDTTPPPVPPTDINPKPGTG
                     NQSSQLLIQINQKLDDGLKNIGTTEPGKAISTLDEIIKQAGQLKADEKNSISEKLGLL
                     VDETLGQISSIDIHKVMGEDSNVIITENKDVLKVEIEDQAITKAIDDLCKNYESIKSL
                     LTENGFDKAANTTLQKGKELKVISGESSKEIDLILTVKSIDALSSNGIDLLVDSKLSL
                     KLPAGAVDLKYTLKYMEDIGQDTKDTEKIKDSKILIVVKELDETDGDVYSVATREMIK
                     ENTGISDASQKFELYLKLLSADGSEKTGIYIEKFNKKLLVKLPLDDSKINNSKINIKS
                     NKLGVYWYNPDKKVWSYVGGKIDTDGKKILFTTDHLSVYAAFEYVKTFNDIQTNWAKD
                     YIEVLASRQIVNGVNNYIFQPNRNVTRAEFTKMLIEALGLVDTSAKAAFADVEEDAWY
                     YKYVSSAQKLGIVNGTAGNMFKPEEIINRQDMAVMAYRSVKAAGLDLLKVKDYKEFKD
                     NKNISSYAKDSVMTLQQAGIIEGSGNSMFMPKNNSARAEAAKIIYMLIEGLQ"
BASE COUNT         2992 a         1961 c         1449 g         3508 t
ORIGIN      
        1 cataatgcac tttattatcc tgaggagcaa gtttttctgc aacatataat ccttcaggga
       61 aaacacccca tgcagcaatc atgctgattg cagcattcat acctttcccg ctttcgccat
      121 atccgctaaa aggagtaatg ccttcttttt cttttaaggt cttcattgcg ttataccaat
      181 catctgtagt cacaggcatt gcaatattat gttttttcag aagatcgttt ctgagaagca
      241 ttacattatt tcctgaaaca aactgataaa tataaggtaa tgagtatact ttaccttcat
      301 cagtggtaac tacctttttt gaaaatgggt actttgcaaa agctttcttt aaatttggca
      361 tatcactgtc aataacagat ttcaaatcaa tgaatgctcc ctgctgtcca tatttttcaa
      421 catcaagagg tgctattaca gcatcaggaa gatttcctgt attcattgca agagtatatt
      481 tttccttgaa gttagcttgt ggtggaatat cccattcaat ttcaatattt gttttctttg
      541 catattcttt taagattgcc aattcgccat attctgtgtc atacataaca tagggcttca
      601 taaatcttaa tttaatgggt ttttcagaga taggaaactt tatgctcggg tcaggcttga
      661 ttaaatctcc ggatgccaga atttctgcag gagtttttgg tgtgctgctt acttctgtag
      721 ttttttcctg agttggagta gttagtttag tttcgttgtt tccatcattg gtttttttaa
      781 ctgtacagga tgcaaataat ccggcacaca taaccaatgc cataactaga gacactactt
      841 taaagaattt ttgtttcata agaatactat cctccctttt ttttgttatt gaattgatta
      901 aaaagataat tttattgcaa ggtgaaatca taacatttat gtgttataga ttcacacatt
      961 gttcaatgtt agtataatca aatgtatttt ttagtgtaaa gtaccaactt gtgcttttgt
     1021 ttaacaaaaa attattatca ttatttgtta aactgtgcat attttgttga tcggcagtat
     1081 aacaaagcag taataattta gcctttgatt gcccctataa gtatgccttt tacgaagtat
     1141 ttctgcagaa aaggatatac cattaatata ggtactgtag ccactacaat agatgcatag
     1201 cggatgcttt cccctataaa ttcctgctct gccgctgacc ctaatccttc tgccatcctt
     1261 gcatttaaca ggttttgcag caatatttcc ctcaatatca actgtaatgg atattttgcc
     1321 ctattattca aatagatcaa tgcattaaaa aacgcattcc agtgccccac tccgtaaaat
     1381 agagtcataa ctgcaataat tggtgctgat agtggaagca caatacttac aaatatcttc
     1441 aagtcattgg caccatctat tatagcagct tcctgaagtg aaaacggtac atttgcagta
     1501 aagaatgtcc tcattatgaa catattccat gtacttactg cacctaaaat aaccattacc
     1561 caaaacgtat caatcaaatg cagcttctgt atcagcaaat atgttggaat aagtccgcca
     1621 ccaaagaaca tggtaaaagt aaaatataat gtccagacct ttctcccata aaaatccttc
     1681 cgtgataaag gatatgcagc catagaagta agtattatat taataaatgt tcctacaaca
     1741 gtataagcaa ttgtattaag atagcttctc cagatatcct gattaatgaa tactctccta
     1801 taaccctcaa ttgtaaatcc tttcggcagc aaacctactt cgttagccat gactgcaaaa
     1861 gggctgctaa aagatacact aacaacatga attaaaggat ataaaaccac tatacatgtt
     1921 attataagta ataggataat aatatggtaa aagattcttt ctccaatcga ttcctttata
     1981 gctttaccca attttgtcac ctccttacca caagcttgtt tcactaactg cacgactgaa
     2041 cttgttaact gcaatcagta atataaaatt aattattgaa ttaaacaatc ctattgctgc
     2101 agtataactg taatttgcat ccaaaatgcc tttcctgtaa acatatgtag aaattacatc
     2161 tgctgtgtca taggttaatg ggctatataa cagcatgatt ttttcaaatc cgacactgaa
     2221 catacttccc attcttaaga taagcataat aattacagta ggaagaattc ccgggaaagt
     2281 aatatgcttc atcttctgcc actttgttgc accatccact gttgctgctt catataattc
     2341 aatatctatt cctgctaatg ctgccagata tattattgat ccccaacctg cagactgcca
     2401 aactcctgat aaaacatata ctgttctgaa ccattttggt tctgcaagga aatgtatggg
     2461 ttgcccgcca atagcttgta taaacctgtt aataaaccct gtactcggag ataagaaaga
     2521 cacaagcata ccgcaaataa ctactacaga aataaaatgc ggcatataaa ctatggtctg
     2581 tacacttctt ttaaatctgt tgctctttac ttcattaatt aataaagcca gtataatagg
     2641 catagggaag cctacaacaa gttcataaat attaatcgcc aaagtgtttc tcagtaaacg
     2701 ataagcaaat ggagattcaa aaaatgattt gaaatgctta agcccgaccc atggactgct
     2761 tataatacct ttaaacgcat taaaatcttt aaaagcaata agtactccat acatgggcat
     2821 ataatggaat ataaaataat aaactattat tggtaatatt aataaatata ggtatcttga
     2881 tctgtaaaag ccgcaagcaa ccgcagatat tccagttttt tttgaagata tccctctttt
     2941 ttcacttgtg tctttatttt tcatggacaa tttgggtgat atcattttag ctcccagtgt
     3001 ttccaattat gttctctcct ttctgcttga caatagcgcc ctccatcagc tacaattata
     3061 aaccccggga agttttactg taaagttgca atttgtgatt gcttttaaca tttactttga
     3121 ttttacatat ttctaactat acaaatttca taaaacagtc caaaatctta tttttattaa
     3181 tacaatccgg actcttaggg tgcacataat ttatctgaag aaaatgcgcc cacaaatata
     3241 ttttatttta caaattccct atagtacgga aattagtagg tgtaatactt tcataacgct
     3301 tgaatacacg ggaaaaattg tgcagactgt tatacccaac ttcctcagca attacggcaa
     3361 cagtcttttc agtattctgc aaaagctcct ttgctttttg caagcgtacc tgatgtatat
     3421 aatcagttat tgaataacca atgtgatctt tcataaagcg gcttaaataa gatggagaaa
     3481 cagaaaaata atcagcgatc ccttctactg aaagcatact gtcactatag tttttattta
     3541 tatactcaat aaccttatcc cttaattcta aatttttaga catctttgca tcttttactt
     3601 catcacacaa ctttaaataa aaatttgaaa cagcactata tatttcatct agagtaccca
     3661 tattatttaa attgtttata aaaatttcta tatttgaact ttttatattt atctcatcag
     3721 cagctttcat agcagtattg ataatatcga agtaaatata ttttatgaaa tcaatagtaa
     3781 caggttcact tttaatggta tgcacaatct catccagaat cttttgaatt tcttcgtatt
     3841 cacccatctt cagaaaatta attaactcct tttcctgttt aaaagaataa caaacctttc
     3901 ttgtaccacg gctgcaaata gcatcatagc taatcactgc atcgcgtccc attattattt
     3961 tatattccag ggcttcacag gcttcgttat aagagtcgct caaaagccat agtttattat
     4021 atagcccgcc aattcctata gtaactgtaa aacccaaatc atttttcaca acatccttaa
     4081 ttttccgagc aatattaaca aggtatacat aattatcatg ctcgaaatta ttaagacccc
     4141 tattgctgat attgttagta ttactggtat tactgatatt actgatatta ctgatgttat
     4201 tcggtatgct gttgtccaat ctccttttat ctaatctgct gtcatatgag atgtcattaa
     4261 gtgaactgtc atttcccaag ctatgattat ttgatctgtc atctactcca attattacac
     4321 taattctata tgcttcatct tgcagagcat atacaaagca atctttatca actaaattct
     4381 caacaatgtt gattaaagtt gcctgtacaa cttccctaat aggttcattt ttttctgaag
     4441 aatttacatt attggaacta tcaacgtcaa gaataaaaac tccatatcta tctttcttaa
     4501 tattcagact taaaaattca agcatttcat ctacactttg ttcatctgta aaactacttt
     4561 ttaataatcg gctaaaaaat gtatttttca taattggtat ctgttcatat atttttttct
     4621 gcagtgactc atttttgctt atatagctaa caatagtgtt atttatcaga tcccattcac
     4681 ttttataccc attgagggtt gtatcatgtt caaatcctct gatagcctta ataatagtat
     4741 taatattatt atagccattt tggacaaaat agagcgcaat tgctacacca agaaataagg
     4801 tcgaaaccaa tataattgtc atcctatttt gaatatcact aactttatcc agtatagtat
     4861 atgaaggtat aatagatata tatttccaat tattttcttg agatttaact gatgatacaa
     4921 caaacccgta ctttgtaata tagcagctgt ccgcttcctg atcttttaat cctgacagca
     4981 aagcatccct ctttccgtca tcaaacagaa agttaccaga tttattaccc attattattt
     5041 tgttatttgc atcaagtaca cacgcatatc cttcgtattg actcaaggaa gtttctattg
     5101 aacgctttat ggaagattca tctacagtga tcatcaacag agccttaggg aaaggatcct
     5161 tcattggtat gggttgaata tatgagataa tacgccttgt taaattgtta aagattgcag
     5221 tttgagaagg tagtatggtt tgagtatgag tattgtataa aatccgttta aattcatcag
     5281 tgctcatttc ctgccacgat aaaacatatt tgttaaatga ctctaaataa tatctgctcg
     5341 tatcactcaa taccatatct gcatctttga aaaaaataaa aatgttatct attgatgaat
     5401 ttgcgttttt atatttgttt agctccttta tagcattgga taaataagga tatatgctta
     5461 ccttttctac attatcgtgg gacaataaat atgaaatatg tggattactt gataaatttg
     5521 atgaaatgtt tgcaaaactt tcaagattgt tatctgtaat gtcacgagtt tgctccagaa
     5581 cccttgcagc tgattgcata atctcctgct caaattggtt aatgaattgg aaatatatga
     5641 aatagctaat aattgttaca ggtaaaaata atactaataa ataagaaagc atgtacttca
     5701 caaaaaccct catcctaaac atgagattgc ccaccttcca aattaaatag aattgttatg
     5761 catatttata ttttatcatt tagttaaatc tatttcaata taaaatttga accgtcccac
     5821 agcttggctt gccggggacg gttctcaaat attactgtaa tttcagcgct aatctaatac
     5881 tgctttgtta tgttgccaat ggaaagcaca tttctggctg gaagacattg cttccaaatt
     5941 gagataggta tagtagtatt agtttattgc agcccctcaa ttaacatgta tataatcttt
     6001 gctgcttcag ctcttgccga attatttttt ggcatgaaca tgctatttcc gctgccttca
     6061 attattccgg cctgctgcag tgtcattaca gaatcttttg catagctgga tatattttta
     6121 ttatctttga attccttata atcttttact tttagtaaat caagtcctgc cgctttaaca
     6181 gatctataag ccattaccgc catatcctgc cggtttatta tttcttcagg tttaaacatg
     6241 tttccagctg ttccattgac aattccaagc ttttgggcac ttgatacata tttatagtac
     6301 caggcatctt cttctacatc tgcaaatgca gcctttgcac tagtatcaac aagtcctagt
     6361 gcctctataa gcattttggt gaattccgcc ctcgtaacat ttctgtttgg ctggaagatg
     6421 tagttattta caccattaac tatttgcctt gatgccagaa cctctatata atcctttgcc
     6481 cagttggttt gtatatcatt aaaggtcttc acatattcaa aagccgcata tacgcttaaa
     6541 tgatccgtag taaataatat ttttttacca tcagtgtcta ttttaccgcc aacataactc
     6601 caaacctttt tatcaggatt ataccaatat acaccaagtt tattactctt aatgtttatt
     6661 ttgctattat ttattttcga atcatcaaga ggtaatttaa cgagtagctt tttgttaaac
     6721 ttttctatat atattccggt tttttcactg ccatctgcac ttaataactt caaataaagt
     6781 tcaaactttt gggacgcatc tgaaatacct gtattttctt ttatcatttc tcttgtagca
     6841 acagaatata catctccatc tgtttcatcc aattctttta ctacaattaa aattttacta
     6901 tcttttattt tttctgtatc ttttgtatct tgtcctatgt cttccatata ttttaatgtg
     6961 tatttcagat ccacagcacc tgcagggagc ttcaacgata atttggaatc tactagaagg
     7021 tctattccat tagaagataa agcatcaatt gatttaactg taagaattaa gtctatttct
     7081 ttactacttt cgcctgaaat aacctttaat tctttcccct tctgaagagt agtattggct
     7141 gccttgtcaa aaccattttc agtaagtaat gatttaatgc tttcataatt tttacataag
     7201 tcatctattg ctttagttat agcctgatcc tctatttcta ccttcagaac atccttattt
     7261 tcagtaatta ttacattact atcttcaccc attaccttat ggatatcaat agaagatatc
     7321 tgtccaagtg tttcgtcaac aagcagtcct aatttttcgc ttatactatt cttttcatct
     7381 gcttttaatt gccctgcctg ctttataatt tcatcaagtg tggatattgc ctttccaggt
     7441 tcggttgttc caatattctt cagcccgtca tctaattttt ggtttatctg tattagcaat
     7501 tggctgctct ggtttccggt tcccggttta ggattaatat cagttggtgg tacaggcggt
     7561 ggtgtagtgt cggagtctga gtctgagtct gagcctgagc cggaacccgg atcaacaggc
     7621 ggcttgcttt tttccagtga cattggaatt acaaaactat tgccttctgc aatattgtag
     7681 ttaaatcctt gggatatatc acttgagttt acataacttc taattaaagt gcttgcgccc
     7741 aggtccaaag ttacttcatt tccgtttaca gacttaatgc cgttaacctt ataggctgcg
     7801 ttttgtacac catcattagt aatatagata tatctgccag ttatttcatt taaatctact
     7861 atatctgcta attcaacagt aagctcatta ttaacactca tctcttttgt aaagctcttt
     7921 acagtaccgg caacagttgc tggcaaatca taattaaaag taccaataat agttccatca
     7981 tttacatatc cataaacctg ttgtccttct ttttcggaat aaactcccag gaatccttta
     8041 aattgaaatt taccatctac tgtataaaca gtttccttat ccagtgaatt aactatataa
     8101 tcagtacgtc cgtttttgag aactacttta actgctttta catctcctgc agctgcctcg
     8161 ccatttactg ttacaggaac ttcgcatatg gattcaatat atcttgcatc tttataaggt
     8221 tcaattactg ctgtaaatgt gctgcttaag ttctgaccgc tgcggtgggc aatcatatat
     8281 ttaagtgacg ctggattacc cggtttctta ggaggtactc catccataat agccacatcg
     8341 tcatatttgc ctagcatagt aagtcttaaa tggatatcca tttcttgtgg cagtactttc
     8401 cttccatcta caacatccca gtctatgctg aagttatttc caggtgctga atctttttct
     8461 acattcttga gccagtggaa tcctgctcct ttataactcc agccagcgac actatcatca
     8521 gaaggtcgta ctccaaattg agtgttaact cctgcataag tccctacaaa attgccttcc
     8581 ggatctttct gtccaataag gttgagacct tctgtggcta ctgcaccttc agccccatgg
     8641 aaactatagt agtggtcgct tcctcctttt actctaaaga aatcaactcc ataggaaatc
     8701 tcatcatcaa ccttgaccat agcaacagtt cttctgtaca tttgagtctg gctgtaagct
     8761 cctgtggaat ctacatccat tacatctact gtatcactat catcgaagtg aattggagct
     8821 ccgccatagt aatcattttg ttttgttttg tctacaacta cagtattatg acttacagtg
     8881 ttgcttaccc actctactct gttaggctga tctcctgttt cctccggata tccaagatct
     8941 ggtgagatat ccaggccaaa tgcatgcact ccaaggttaa gctggtctct atgagcatgg
     9001 gaagtatttg ttcttccgta gtacatccaa aagtctctta aggtatttaa taaagtagta
     9061 tcattcttta cttttagaga ttcattgtca aagagaacaa tatgaataac acccatctta
     9121 tatcctgttg cattttcatt cttgtctaca ccctcaaaag taataacatg ctcaccggca
     9181 gtaagttcaa gagttgcaac tggttcaagg tttgagtcag ctccgcttcc cccgtagaaa
     9241 tcaagagttt ttacctttgt tccatcgata ctgatatcat atataccata actacctgcc
     9301 ctaaaaggat ttaaatcaac ttcatatata tctgtctttt ctacattaaa tctgaaacta
     9361 attttatctc ctattttatc agactcaaat tgaattgttc cattgcttgc aaaatgttta
     9421 aatcctattg tagcatcaaa ggcacttaaa tttgcaaagg gaatctgaat tccaaagtac
     9481 tgtttgttgt tttttccatc tctaagaact gagaagccat accctgccag gtttgtactt
     9541 cctaaatcca attttccttt ttcagctatt acattttcaa tagccggtcc tatactctca
     9601 ggatcttgtg taaatatatc tccatgcaat ccaccagctg agttattatt taagaagtaa
     9661 gccagttgag caaggacagg gtctccaaat ttttcaaatc cctttacaga ctctgatatg
     9721 cttaccataa gtcccgggtt gcctgttcca cctgagtctc ctatctgagc agtgtaacgt
     9781 tcagccagta taagcgggta aaatgctgag aacattttca taaacttagg attcttgtat
     9841 aggtctgccc cgggatatcc ttcatatcca tccagaacat tggcgacctc caataattgc
     9901 cttagccata
//
LOCUS       DAOSDD010000172         9905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2579_length_9905_cov_5.807310, whole genome shotgun sequence.
ACCESSION   DAOSDD010000172 DAOSDD010000000
VERSION     DAOSDD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_9905_cov_5.807310"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1389
                     /locus_tag="PK733_13790"
     CDS             358..1389
                     /locus_tag="PK733_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOJ11649.1"
                     /translation="MELYNDNLSNLENKGFRKPGIPMTGIVYSIVVTLFITIAYRVQH
                     KNFYLGVLATEIILIMLPPLIALFIFKYDIKKVLRLNKIGVINLLLIFCIMVFSIPVV
                     GAFNLANLWLVKYIFGKAVLVQPPIASGYGGLLLSVLVVGVSAGVCEEVLFRGFIQRG
                     LEKFGAGTSILISAFLFSIMHVQFEKLFGTFLLGTLIGFIVYRTNSLYGGIFAHFVNN
                     TIGVLVLFASSKLSEFAKLSGVEPATDVGQIFESFENMPLPQLIIIIVVWSFIILTCA
                     AILAALVFALIKNTSKPQSAMASGNAGLFTFLPGIFIISSMYFLEGLMLKGVENSTAE
                     FILRILGAK"
     gene            complement(1603..2619)
                     /locus_tag="PK733_13795"
     CDS             complement(1603..2619)
                     /locus_tag="PK733_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ11650.1"
                     /translation="MNENIFSLIRREYERKQRLAQEKLRNKKSELSLKVPEIAELENK
                     IQECGLAYSKLILTGKSRFDVEASALLLDMDKLKNKKRDLLLSCGYPANYLEPEYECE
                     MCKDTGFIDDNGFSVKCSCYRQQLINYLYDQSNLSLVKLENFDNFNEQYYPDVVDENR
                     YGAKISPRVNILNIKERCLAFINNFDSPTEKNLFFSGPTGVGKTFMASCVARELLDRG
                     KTVLYQTAPVLFNTINEYKFRFSGNEEYSDAGYRNIFEVELLIIDDLGIEPSSPARYA
                     ELLTILNTRQVNNITRPCKTIISTNIGPKQLYEFYTERVTSRIVGNFNRLMFIGEDIR
                     ILKK"
     gene            complement(2640..3617)
                     /locus_tag="PK733_13800"
     CDS             complement(2640..3617)
                     /locus_tag="PK733_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOJ11651.1"
                     /translation="MYFQEFKSVLYSDTLVPDIFISEYLPSMPCEHVKVYLYCLFLSK
                     YNKKATTEDISKTLGMGYDSVSNAISALENLGIVGRKDNSIAIFDLKEKEINKIYRLK
                     TTSTPEEALFSSERNNKRRETIAAINKNFFQGLMSPSWYTNIDAWFDKYKFEEDVMYA
                     LFQHCYDHRGLSPNYIVKVADNWHSKKIINSFDLDEYFIQYQKIRDIKLKTVKKLNLN
                     RFLTEYEEDYIDNWVENFRFSFEIIDLALKKTTSKTNPNFNYINAILKDWADNNLKTK
                     EEIIEYEKIKKKNYGKKQSQDTTVPQRDNFEQRKYDDEYYDKFFSNTGK"
     gene            complement(3767..5002)
                     /locus_tag="PK733_13805"
     CDS             complement(3767..5002)
                     /locus_tag="PK733_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ11652.1"
                     /translation="MHTNTSEQFFERSKKVIPWGSSTCSKAAYLQPYEPVAIVKGKGC
                     RVWDIEGREFIDFRNGLGPITLGYQFPEVDEAIRTQLESGILFGHPSILECEVAEMLT
                     EVIPCAEQVRFLKTGGEAMAACIRIARGYTDRDHIVQVGYNGWLNSLSAGGPVLPRQS
                     GGSFPSGIPGCLSELHHSCGWNDIDAIKKVFEDVKGNLAAIVIAADYKQMEKGSTYYR
                     EVRDFADKNGVLLIFDEIVTGFRIAMGGAQEYFNVTPDMAVFSKGIANGMPISVYAGR
                     REVMQKCDKGNGIVISSTFAGEALSLAVAKAVINVYKSCNVVEHLWKQGEKMWTSLNK
                     IFEDYNIPVEIKGFWPCPTFTSKADAPADIISKFMYFAYKHGVSLYNVSYVNFSHKDN
                     DINEALQRLENACKELSVN"
     gene            complement(5420..5611)
                     /locus_tag="PK733_13810"
     CDS             complement(5420..5611)
                     /locus_tag="PK733_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11653.1"
                     /translation="MEKCIYCGSSNIEKELSVGAGNFKTGLKYLNFITPQVEWFYANL
                     CKDCGSVRIYVKETNRKWS"
     gene            complement(5739..6074)
                     /locus_tag="PK733_13815"
     CDS             complement(5739..6074)
                     /locus_tag="PK733_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOJ11654.1"
                     /translation="MELGSNGFSYRGWFLVERITVLNVYEELKSGEKVTTHHIHIVKV
                     DSSDWNNHINFRDYLNAHPSIAKAYEAIKVKLASEHPYDEDRKKYNDGKNDFINQTLK
                     YAAEWLRQS"
     gene            complement(6029..6196)
                     /locus_tag="PK733_13820"
     CDS             complement(6029..6196)
                     /locus_tag="PK733_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11655.1"
                     /translation="MPLGLKRNIVELIDHDTEWEKLVIIDIAVAVDDFKKFKPLIWNW
                     EVMVSHIAAGF"
     gene            complement(6243..7070)
                     /locus_tag="PK733_13825"
     CDS             complement(6243..7070)
                     /locus_tag="PK733_13825"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ11656.1"
                     /translation="MAVIETGWERNKRTHFNDIVEDYDKIRPEYPREIFEDVMKFPNV
                     LTGKSAIEIGAGTGKATHPFLDAGYDVTAVEIGSNMADFLLKRFNAYKGFNVVVSSFE
                     DAILPESSFDLIYAATAFHWVDAEIGCPKAFRLLKDGGTIALFRYNAIAGDGDELYEE
                     IQAIYEKHYYSYYKSNTRPIKKSKEDFEKPSEIYISYRFEDLKTYGFKDISMKFYDGY
                     KTYNADEYIVLLETMSDHKCLPNSNKMALYTGIKEAIIKHGNHHKVDYIFQLYMGRK"
     gene            complement(7173..8762)
                     /locus_tag="PK733_13830"
     CDS             complement(7173..8762)
                     /locus_tag="PK733_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11657.1"
                     /translation="MPKIGVTVYSLLISCPGDVEEEVGIIREVVENFNRMFGEINNIQ
                     IAVKHWSTDAYPQSGGKPQDLLNKQFVNDCDAALAVFWTRFGTPTDKYQSGTEEEIEE
                     MIKSGKQVFLYFADKPITPSTIDMAQYEKVQAFRKKYKDRGIYWSYSDLNELNKLLLN
                     HISKYFLNILTSTETNDAERPQLVVRGVYDNKPSESLHILRGRWLDSGLFVEEVKEIG
                     NLYEKISGIVLPKMEVEEKKEPAISPNESIAKLAKQFSLAAKDLQMANKAPAVVSDKI
                     KDCILNYSQHNNMELSDDFFYLANLKKSTLSFNINPFGSSAPSYDGTEDEISKHHLIV
                     ELYWKIVEYNEKTEFYAKLQNYFVIKCIVSNIGKSYDEDVDVQLKIPKGCICKTSQLP
                     IPGYSFIEEFNDQSIADYIYKIRKAISIDEYSNYPAYKSLKSFGMPVGLLNKKTAEEK
                     YSEEKRCYQSTIDEIFCYEFFETEEYDILAFNITYLKQNTNMAIPSLLYFKSEPKEIV
                     YEIRSKHSPDVVSGKIEVQGG"
     gene            complement(8936..9145)
                     /locus_tag="PK733_13835"
     CDS             complement(8936..9145)
                     /locus_tag="PK733_13835"
                     /inference="COORDINATES: protein motif:HMM:NF020421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HOJ11658.1"
                     /translation="MIKRKLTITYQYYPSNTVIPVLRVQGCWLEKLGFKVGDKVEIYQ
                     VREDELIIWKVDNDIKINSTGYLVG"
     gene            complement(9169..>9905)
                     /locus_tag="PK733_13840"
     CDS             complement(9169..>9905)
                     /locus_tag="PK733_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ11659.1"
                     /translation="DSIKEPRSKKYEEPKYKALSDDELKKIFEAVNDNPMIKTATIIM
                     NYTGMRPGEVFALKIGDIDFANKTISVNRALSLERDVDIETKKYIGRRRAIIKEIKNE
                     RGGKVENAKRTLKVSDNVLETIENYLRDILELIEKRKKHGMEDFIFSGVNGNLVILEY
                     YQQVYERQLAKKGLDSKKLNLYRFRHTYCTRLAEMGTDPTIMMQMMGDNTIDIVMKVY
                     RSVKKEDILAASSEYAAKMDATLVEA"
BASE COUNT         2894 a         1942 c         1536 g         3533 t
ORIGIN      
        1 ataatcagga ctttctggta tatataaatg ctattacagg gaaagaagaa aatatattaa
       61 tgataatcaa tacaccaaat ggaattctga caatgtgaaa tgatatctga ctacatgaaa
      121 cggtaatggt attttagcaa tatggcaatg tattaaatat ttatatactt caaaagtgga
      181 agagaaacag gtacatttat aaattagaga ggtacctgtt tttcctttcc ggaatttatc
      241 gcagcgacca cttatttgtt tgcagttata ccatgttata tggtatgata tggtgtataa
      301 aaattatata ttttgtagta agcaatatac gttgcaataa gcaaggaggg actgtttatg
      361 gaattgtata atgataattt aagtaactta gaaaacaaag gttttagaaa accaggcatc
      421 cctatgacag gaatagtata ttccatagtt gttactctat ttattactat agcatacagg
      481 gtccagcata agaatttcta tttaggggtg cttgctactg aaataatctt aataatgctt
      541 cctcctttga ttgcactatt tatctttaaa tacgatatta aaaaagttct caggctaaat
      601 aagataggag taataaatct tctcctgata ttctgtatca tggtgttttc tattcctgtg
      661 gttggcgcgt ttaatttagc taacttgtgg ttggtaaaat atatttttgg caaggcggtt
      721 ttagttcagc cccctattgc ttcaggttat ggggggctgc ttttaagcgt attggttgtg
      781 ggtgttagtg cgggtgtatg tgaagaagtt ttattccggg ggtttataca aagaggactt
      841 gaaaagtttg gagctggaac atcaatatta atttccgctt ttctttttag cattatgcat
      901 gtacagtttg aaaagctttt cggaacattt ttacttggta cccttattgg ctttatagta
      961 tatagaacaa attctcttta tggaggtatt tttgcacatt ttgttaataa taccataggt
     1021 gttttggttt tatttgcttc aagcaagctt tcagagtttg ctaaattatc gggagtagag
     1081 cctgccacag atgttggtca aatatttgaa agttttgaaa acatgccact tccacaatta
     1141 ataattataa ttgttgtatg gtcttttata atattaacat gcgcagcaat actggcagcc
     1201 cttgtatttg ccttaatcaa aaatacctct aagccgcaaa gtgctatggc ttcaggaaat
     1261 gcaggattat ttacctttct gcctggtatt tttattataa gctccatgta tttccttgag
     1321 ggactgatgc taaaaggggt tgaaaacagc actgctgaat ttattttaag gattctggga
     1381 gcaaagtgat aaaagggcaa agtgataaaa cggcaaagtg atataaaact tgacgatgat
     1441 tacgtatttt caggagttta gccgaaagtt ccataacttt agtcactggg attaagactt
     1501 aattagtacc cgaaaaaatt agccttgctt gcatgcttgc atatcaggca aggctaagct
     1561 aaggttaagg ctaggtaagg caagaactgt atctgtctgc tcttattttt tcagaatccg
     1621 aatgtcctct cctatgaaca tgagcctgtt aaagtttccc acaatacgtg atgtaactct
     1681 ttcagtgtag aattcataca actgttttgg tcctatattg gtggatatga tggttttaca
     1741 tggtctggta atattattta cctgccttgt attcaaaatg gttaatagct cggcatatct
     1801 tgcagggctt gatggttcaa tgcccaggtc atcaattatt aaaagctcca cttcaaaaat
     1861 attgcgatat ccggcatcac tgtattcttc atttcctgaa aatctgaatt tatattcatt
     1921 tattgtatta aacaaaaccg gcgctgtctg gtaaaggact gtcttacccc tgtcaagcaa
     1981 ttccctggct acacagcttg ccataaaagt tttccctact cctgtaggcc cgctgaaaaa
     2041 aagatttttt tcagtaggag agtcaaaatt atttataaaa gcaagacatc tttcttttat
     2101 attcagtata ttcacccttg gtgatatttt agcaccgtac ctgttttcat caactacatc
     2161 agggtaatat tgttcattaa aattatcaaa attctcaagc ttgaccagac ttagatttga
     2221 ttggtcatat aaataattta ttaattgctg ccgatagcag gagcatttta cagaaaaacc
     2281 gttatcatct ataaaccctg tatctttaca catttcacat tcatattctg gttctaaata
     2341 attggcagga tacccgcagg acagaagcaa atctcttttt ttattcttca gcttatccat
     2401 atcaagcagt aaagcagagg cttccacatc aaatctgctc ttaccggtta aaataagctt
     2461 gctataggct agtccgcatt cttgtatttt attttccaac tcggctattt caggcacttt
     2521 taaagaaagc tcactttttt tgttcctgag tttctcctga gccaatcttt gttttcgttc
     2581 atattctctt ctaataaggc taaaaatatt ttcgttcatt gaaaaaacct gcctgcttcc
     2641 tacttacctg tattactgaa aaatttatca taatattcat catcatattt tctttgctca
     2701 aagttatctc gttgtggtac tgtcgtatcc tgtgactgct ttttcccgta atttttcttt
     2761 ttgattttct catattcaat tatttcttct tttgttttaa gattgttatc cgcccaatct
     2821 tttaaaattg cgttaatata attaaaattc gggtttgttt tagatgtggt ttttttaaga
     2881 gccagatcaa ttatttcaaa gctgaatcta aaattttcaa cccagttatc tatgtaatct
     2941 tcttcatatt ccgtaagaaa tctattaaga tttagcttct taaccgtttt caacttaata
     3001 tctcttattt tttgatactg aataaaatac tcatccaggt caaaactgtt gataattttc
     3061 ttactatgcc agttatcagc aacctttaca atatagttag gcgacagacc cctgtgatca
     3121 taacagtgct gaaacagggc atacataaca tcttcctcaa atttatactt gtcaaaccat
     3181 gcatctatat ttgtatacca ggaaggtgac attagtcctt gaaaaaagtt tttgtttata
     3241 gctgcaattg tttctctgcg tttattattc ctttctgagc taaacaatgc ttcttcagga
     3301 gtagatgtag ttttaagcct gtaaattttg tttatttctt tttccttaag gtcaaatatt
     3361 gcgatactgt tgtctttcct gcctactatt ccgaggtttt caagagcgga tatggcattg
     3421 ctgacggaat catatcccat acccagtgtt ttggaaatat cttcagtagt agcttttttg
     3481 ttgtatttac tgaggaaaag acagtataaa tataccttaa catgctcaca gggcatggat
     3541 ggaaggtact cgcttataaa aatgtctggt accagagtgt ctgaatataa aactgactta
     3601 aattcctgaa aatacatgct tagcatcctt tctacacaat aggttactta ctcactaagt
     3661 atatcatata cgcttgccac aattcaaaaa aggtaaacct aatctgagaa ttttagtgca
     3721 gtatttgcct tgtttaatct tgatttattt gtttttcagg acagcttcaa ttcacagaaa
     3781 gctctttgca cgcattttca agtctttgta atgcttcatt aatatcatta tctttgtgac
     3841 tgaagtttac ataagataca ttatataaag ataccccatg tttataagca aaatacatga
     3901 atttagaaat aatatcagca ggggcatcgg ctttggaagt gaatgttgga cacggccaaa
     3961 aacctttaat ttcaacaggt atattataat cttcaaatat cttatttaat gaagtccaca
     4021 ttttctctcc ctgtttccaa agatgctcaa ctacgttgca ggatttataa acattgatta
     4081 ccgcttttgc tacagcaaga gaaagagctt cacctgcaaa tgtagacgaa ataactattc
     4141 cattgccttt atcacatttc tgcatgactt cccggcgccc tgcataaaca gatataggca
     4201 ttccatttgc aatacctttg gaaaatacag ccatatcagg tgttacatta aagtattcct
     4261 gagcacctcc catagctatc ctgaagccgg taacaatttc atcaaaaatc agcagaacac
     4321 cgtttttatc cgcaaaatcc cgtacttctc tataataggt agaacccttt tccatttgct
     4381 tgtagtctgc agcaattact atagcagcca ggtttccttt tacatcctca aaaacctttt
     4441 ttattgcgtc tatatcattc catccgcatg agtggtgtaa ttcagataag cagcctggaa
     4501 ttcctgatgg aaaggaacct cctgattgtc tgggtaatac gggtcctcct gcagaaagac
     4561 tattgagcca tccattatag cctacctgga caatatgatc tctatctgta tatcccctgg
     4621 caatgcggat acaagccgcc atagcctctc cgcctgtttt aagaaatctt acctgttctg
     4681 cacagggtat tacttcagtg agcatttccg ctacttcgca ctcaaggatg cttggatgtc
     4741 cgaataatat gccggattca agctgggtac gtatggcttc atctacttcc gggaattgat
     4801 agccaagggt tatgggacct aagccattac gaaaatcaat aaattcacgt ccctcaatat
     4861 cccatacacg gcaacctttt cctttaacta tggcaacggg ttcatatggt tgaagatagg
     4921 cagctttaga gcaggtactt gagccccagg gaataacttt ttttgacctt tcaaaaaatt
     4981 gttcagaagt atttgtatgc attataaatt ctcctttatt ttcctattgt tttgacctta
     5041 tgatcatgct gcttaaatta tatcataaaa taagaaaata aaaacgacag aaaatatgtc
     5101 taaaattcat agtgtgcgta tgctaaaaac tcagatcttc tgtccctgta aacctcaaaa
     5161 ataatcagag cctgtaagat tttacactta caggctctga ttatttttgg cgcgcccagg
     5221 agagttcgaa tccccagctt tctgatccgt aggctaacag tcgatatctc tcaaacccta
     5281 ttggaatcaa cctctaactc tatatatcac tacatttcag gttagccatt gttagcctca
     5341 actttttgac cttctgactc tgttagccat taagtagttt gcagatcctg atgcttcgca
     5401 taatataaag attgtgacat taactccatt ttctatttgt ttcttttaca tatattcgaa
     5461 cactgccaca gtctttacac aaattagcat agaaccactc cacttgtggg gtgatgaaat
     5521 taagatattt caagcctgtc ttaaaatttc ccgcccctac agataattct ttttcaatat
     5581 tactggaacc acagtaaata cacttttcca tttcactcct ccatataaat cttttgtttt
     5641 tttaaaatta gtgttgcaca atattataca attgtgaagt aaaacaacat ctggtaatgc
     5701 ttattatgca ccgctacatg aacagtaata tgtttctgtc aagattggcg cagccattca
     5761 gcagcatatt ttagtgtttg gttaataaaa tcattcttgc catcgttata ctttttccta
     5821 tcttcatcgt aagggtgttc agaagccaac tttactttta tggcttcgta ggcttttgcg
     5881 atagaggggt gagcattcaa ataatcgcga aaatttatat ggttattcca atcactacta
     5941 tcaactttta cgatgtggat gtgatgtgta gtaacctttt cacctgactt taattcctca
     6001 tatacattaa gtactgtaat acgttcaact aaaaaccagc cgcgatatga gaaaccatta
     6061 cttcccaatt ccaaattaac ggtttaaact tcttaaaatc gtccactgct acagcaatat
     6121 caataatcac aagtttttcc cattctgtgt catggtcaat gagttctact atatttcgtt
     6181 tcaaccctaa tggcatatac gaaacctcca gtatttaatt caacgctctc tgtagaaaat
     6241 gcctattttc tgcccatata taattgaaaa atataatcca ccttatggtg attgccgtgt
     6301 tttatgattg cttcttttat accagtataa agagccattt tattgctgtt aggtaagcat
     6361 ttatggtcag acatagtttc aagcaaaaca atatattcat ctgcgttata tgtcttataa
     6421 ccatcataaa atttcattga tatatccttg aaaccataag tttttaaatc ctcaaatcta
     6481 taggaaatgt aaatttctga tggcttttca aaatcttctt tcgatttttt tatcggtctt
     6541 gtatttgatt tgtaatagct ataatagtgt ttttcataaa tagcttgtat ttcttcatat
     6601 aactcatcac catcaccagc aatagcatta tagcgaaaca aagcaatcgt tcctccatct
     6661 tttaataagc gaaatgcttt cggacaccct atttccgcat ctacccaatg aaaagctgtc
     6721 gcagcatata ttaaatcaaa gctactttct ggtaatatag cgtcttcaaa agaagaaaca
     6781 acaacattaa agcctttgta agcattaaat cttttcaaaa gaaaatctgc catatttgaa
     6841 cctatctcaa cagcggttac atcatatcct gcatcaagaa aaggatgagt ggcttttcct
     6901 gttcctgccc caatttcaat ggctgatttt cctgttaaaa cattgggaaa tttcataaca
     6961 tcttcaaaaa tttctctggg gtattctggt cttattttat cataatcttc gactatatcg
     7021 ttaaaatgtg ttcttttatt gcgttcccaa cctgtttcta taacagccat cttacaccta
     7081 cctaatataa atttttaact tcactatcta taaataatac ctggttctca ttataataca
     7141 aatatgtatc atcataattc ttctgataac agctacccac cttgtacttc gatttttcca
     7201 gatacgacat ccgggctatg tttcgaccta atttcatata caatttcttt aggctcagac
     7261 ttaaaataca acagagaagg gatagccata tttgtattct gttttaaata tgtaatatta
     7321 aatgcaagaa tatcatattc ctctgtttca aagaattcat agcagaatat ctcatctatc
     7381 gtactttgat aacacctctt ctcttcactg tatttttctt ctgccgtctt tttattaagc
     7441 aaaccaaccg gcataccaaa gcttttcagt gacttatatg ctgggtaatt tgaatactca
     7501 tcaatggaaa tcgcttttct tattttgtat atgtaatctg caattgattg gtcattaaat
     7561 tcctcaataa acgaataacc tggtatcgga agttgtgaag ttttacagat gcatcctttt
     7621 ggaattttca actgaacatc aacgtcttca tcataggatt tacctatatt actaacaatg
     7681 cattttataa caaagtaatt ttgcagctta gcatagaatt cagttttttc gttgtactca
     7741 acaatcttcc agtacagctc cactatcaaa tggtgcttac taatttcatc ctctgttcca
     7801 tcataagatg gagctgaact accaaatgga tttatattaa atgataatgt actctttttt
     7861 aagttagcga gatagaaaaa atcgtccgat agttccatat tattatgctg tgagtagttt
     7921 aaaatgcaat cttttatttt atcactgaca acagcaggtg ctttgttagc catttgcaag
     7981 tccttcgcag ccaaggaaaa ttgctttgct aacttagcta ttgattcatt tgggctaatt
     8041 gccggttctt ttttttcttc aacttccatt ttaggtaata caatgcccga tatcttctca
     8101 tataagtttc ctatttcttt tacttcttct acaaacagcc ccgagtccag ccatctcccc
     8161 ctaagaatat gtaaagattc agagggttta ttatcataaa caccgcgtac gacaagttga
     8221 ggtctttctg catcatttgt ttccgtactt gttaaaatat tcagaaagta tttacttata
     8281 tgatttagaa gtaacttatt tagctcattt aaatctgaat atgaccaata aattcctcta
     8341 tctttatact tctttctaaa tgcctgtact ttctcatatt gggccatgtc aattgttgaa
     8401 ggtgtaatag gtttatctgc gaagtaaagg aatacttgct ttccgctctt aatcatttct
     8461 tcaatctctt cctcagttcc tgactgatac ttgtctgtag gagtaccaaa ccttgtccaa
     8521 aatacagcta atgcagcatc acagtcgtta acaaactgtt tattaagcaa atcttgtggc
     8581 tttcctcctg attgtggata ggcgtctgta gaccaatgct taacagcaat ttgaatatta
     8641 tttatttctc caaacattct attaaaattt tccacgacct cacgaataat tcctacttct
     8701 tcttctacat ccccaggaca tgagattaat aaactatata cagttacccc tatctttggc
     8761 attttgctgt ccctaccttc ttatttctat attattctta gtattataag tacttcaaac
     8821 tgaccggcct atgcatagac tgcattcttt ctcgttcata tttctactat cagttccatt
     8881 atcagggatt attaccagca tgtcaataat ccctgattca atatttatac tcacgttagc
     8941 caaccaaata tccagtgctg tttattttta tatcattatc caccttccat ataatgagct
     9001 catcctctcg cacctgataa atctccactt tatcgccaac cttgaatcca agcttctcga
     9061 gccagcaacc ctgtacacga agtacaggga ttactgtgtt gctgggatag tattgatagg
     9121 ttatagttaa tttcctcttt atcatactga aattctcctc cagctttcct atgcttccac
     9181 cagggttgca tccattttag ctgcgtactc actgcttgct gctaagatgt cttccttttt
     9241 gactgaccta tatactttca tgacaatgtc tattgtgtta tcacccatca tttgcatcat
     9301 tattgttgga tccgtaccca tttcggcaag tctggtgcag tatgtatgac gaaaacgata
     9361 tagatttaat tttttgctgt ccagaccttt ttttgcaagc tgcctttcat atacctgctg
     9421 gtagtactca agtattacaa gatttccatt aacaccactg aatatgaagt cctccatacc
     9481 atgtttcttt cttttttcta tcagctcgag tatgtctctg agataatttt ctatagtttc
     9541 aagtacatta tcagaaactt ttaatgtacg tttcgcattt tcaaccttac ctccacgttc
     9601 atttttaatt tctttaataa ttgctctacg cctgcctata tatttcttgg tctcaatatc
     9661 tacatccctt tcaagactca aagccctgtt aacactgatt gttttattag cgaagtctat
     9721 atcgccaatt ttcagagcaa acacttcacc cggtcgcatc cccgtgtagt tcattatgat
     9781 ggttgccgtc tttatcatag ggttatcatt aacagcttca aatatctttt tcagttcatc
     9841 atcgctcaat gccttgtact taggctcttc gtacttttta gacctaggtt cttttatcga
     9901 atcca
//
LOCUS       DAOSDD010000173         9854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2593_length_9854_cov_5.258088, whole genome shotgun sequence.
ACCESSION   DAOSDD010000173 DAOSDD010000000
VERSION     DAOSDD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_9854_cov_5.258088"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK733_13845"
     CDS             <1..383
                     /locus_tag="PK733_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HOJ11660.1"
                     /translation="DFRIPNDWARLTATCHHNSPELKNLAKKFVEDKVKREPYLFYLW
                     GHSYEFETDYNWNVIEDFAEFIGNRDDIWYATNIEIYDYIEAYNRLIFSVDGRRVKNP
                     SSRKIWFEYSKEIIEIGAGEMIEI"
     gene            826..1605
                     /locus_tag="PK733_13850"
     CDS             826..1605
                     /locus_tag="PK733_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ11661.1"
                     /translation="MDLNLKDKVAIVTGVCGVKGVAGVGAAITRVFAEQGINVVANYI
                     VDGDDVKNYVDELNKKHGTKCMAVYGDISKPEDIDNIIEKAIQAYGKIDILVNNAAIL
                     PVTNIIDMPDEEWERVIKINLNGTFFFCKRFAKQLIKLNRPGRIVNITSKSGFFVTSP
                     GRSHYATSKGGIITLTKALAREFTQYGIIVNAIAPGILRTPLNNELLEDKEIERNYVK
                     RIPLGRLTDPEEIANIVSFLVSEKSESIVGTTIDATGGMLI"
     gene            1668..2687
                     /locus_tag="PK733_13855"
     CDS             1668..2687
                     /locus_tag="PK733_13855"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ11662.1"
                     /translation="MAIIVKKQTSGNKKNIDKKDMEQHIYWQAHQSTSTVMPENTMAA
                     FMYAWELGGIPEADIRTTSDGVIICMHDSTPARTTDAPDSQRSLPVSSFTLEQVKKWD
                     AGIKTGELYRGEMVPALKEAFEVMKQKKDRLLYLDLKEVDLGSLGKLINEYGVNNQII
                     FTHYNEDNCRQMYKTVEDIKTMLWIGGTPDKIKGKFNKVLSENFYGLNQIQIHLYSRK
                     DNKKDNKKDSNKDSNEDNNNDSNKGINKVNNDKVIGIDYDNKNSNMALTCEEWPYEID
                     KAFLEYAYDKTLTAGLDLEVLPFEFDDSSIHTILNIGIRWFAVDFPERFISSVKKWED
                     KLTSK"
     gene            3017..3748
                     /locus_tag="PK733_13860"
     CDS             3017..3748
                     /locus_tag="PK733_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ11663.1"
                     /translation="MSGHSKWSNIKRKKEKTDAQKGKIFTKLGRELAIAVKEGGSDPE
                     ANTKLQDVIAKAKAANMPNENIMRSIKKASGDMDADNYEEISYEGYGPGGIAIIVEAM
                     TDNRNRTAGDIRHFFDKFGGNLGQTGCVSFMFDKKGVILIERTDDVDEDTLMMEVIDL
                     GADDFNPQDEYFEILTDPGEFSAIRGALEKKGYKFMEADVEMVPQTVAVLTDPKHIEQ
                     MNRLIENLDDLDDVQNVYHNWEEDE"
     gene            complement(4033..4224)
                     /locus_tag="PK733_13865"
     CDS             complement(4033..4224)
                     /locus_tag="PK733_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOJ11664.1"
                     /translation="MANNRSKTAFENMKYEIANQVGVNLKQGYNGDIAARDAGKIGGN
                     IVKKVFESYTGNNYNVNKQ"
     gene            complement(4466..4681)
                     /locus_tag="PK733_13870"
     CDS             complement(4466..4681)
                     /locus_tag="PK733_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOJ11665.1"
                     /translation="MNRTPIDRVALVLVIIGALNWLLVGLFSFDLVGAIFGGNTTFLS
                     KTVYTLVGLAGLYSISLLFREVNTATE"
     gene            5190..6134
                     /gene="ytxC"
                     /locus_tag="PK733_13875"
     CDS             5190..6134
                     /gene="ytxC"
                     /locus_tag="PK733_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HOJ11666.1"
                     /translation="MQFLSIDVEKCADDVLEFISKELIQLKNDKIDYSIDRVNNEGID
                     SIICKLIENKSQVKAPKQAYKALMLNVSNALADYIIRYYEEKLIRRIVNSNYCYFSQT
                     ERKDILKEAFLTVRNEEKSFLNTLLQIRRRNIIVKNLMEYFENSNNVILDGFVNFRLK
                     DYIRDLEEIVEKAVDSFLMEKEYKEFIKLLKYFVDIQEPKFDNIHVIAESEGKYNLLD
                     DRYEEITNECIKEYLNEISEGEINYDDLLVSSLITLAPKKITIHRTAHFKNKELLETI
                     RNIFQKNVVICKGCGICNATSATNKKSIKEAEIPDNIY"
     gene            6433..7266
                     /locus_tag="PK733_13880"
     CDS             6433..7266
                     /locus_tag="PK733_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ11667.1"
                     /translation="MRVVGFVGASGTGKSHRALQVASSRGIEYIIDDGLLIKGNSVIA
                     GVSAKKESTKIGSIKRALFIYDDHAKSVKKAIELANPNAILILGTSDGMVESIAKKLD
                     LPEVSQKVYIHEVAKEAEINQALSTRRNEGKHVIPVPTFEIKKDFSGYFLDPLQIFRR
                     KGKGRFQLVAEKSVVRPTFSYMGKYTISDYTIYQIAEYVIYDIKGISRISRFKVENGN
                     EGITIDIDLILIYGYPIKSLLETVQQRVKLEIEKITALNIKALNLTAKGLYIQENSLS
                     S"
     gene            complement(7449..8225)
                     /locus_tag="PK733_13885"
     CDS             complement(7449..8225)
                     /locus_tag="PK733_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOJ11668.1"
                     /translation="MKEQIYTIPVVDAFNEDSECAICTLERKLEDQYIEYFLGPSLME
                     PDCRIETNEKGFCTRHFELLYNRQENRLGLGLIVDTHLQEQNKELKKMYSKKISYIQK
                     ESETTLVKSISNKLTSKKTSTEKFVENMVQELASLEKKCMICGKMSYTMERYIDVIFY
                     LWFKEREFRDLFNNNKGFCLVHFRQLLESMGKYLNSRDMAIFADNLLKMQLKNMERVQ
                     QEVNWFTEKFDYRNNDAPWGNSKDALPRSIQKITGYCNLK"
     gene            8353..9306
                     /locus_tag="PK733_13890"
     CDS             8353..9306
                     /locus_tag="PK733_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOJ11669.1"
                     /translation="MEYKGYKIQDHQSSGGGKLIRVYDVRDFNPVHIFECGQCFRWYR
                     EHDGSYTGVAKGKVANIFYDAYDYCSEHNDGHGSDKGGTLYIRNADMEDFKNVWYDYL
                     DLGRNYGDIKEALMRKDDIMKKAVEFGYGIRLLRQDFWETLISFIISANNRIPMIMKV
                     VASISESFGKKILINEKAYHTFPDVENLASASIEQLAVCRAGFRCKYIDITSKMVEEG
                     CLKPSELYLVNTDEAREMLKQLPGVGDKVADCTLLYSGIKYDVFPTDVWVKRVMEELY
                     FHREATLKEIQKFAANYFGELAGFAQQYLFYYARENKIGAK"
     gene            9389..9649
                     /locus_tag="PK733_13895"
     CDS             9389..9649
                     /locus_tag="PK733_13895"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOJ11670.1"
                     /translation="MYYLVLVDSGNYAIRLSRIMAQKGYPVEVVSTPCKIAKNGCGYC
                     LRLIPEYKDTLINEGKTNAINIREIYSVTNASYKNIYKKIYP"
BASE COUNT         3485 a         1429 c         1900 g         3040 t
ORIGIN      
        1 acgatttcag gattccaaat gattgggcgc gattaaccgc aacttgccac cataactcac
       61 ctgaacttaa aaaccttgca aaaaagtttg tagaagataa agttaagcgt gaaccttatt
      121 tgttctattt atggggacat agttatgaat ttgaaactga ctataattgg aatgtaattg
      181 aagattttgc tgaatttata ggtaacagag atgacatttg gtatgcaaca aacatagaaa
      241 tttatgatta tattgaagct tataaccgat taatatttag tgttgacggc agacgagtaa
      301 aaaatccatc tagtagaaaa atttggtttg aatattcgaa agaaataatt gaaatcggag
      361 caggggaaat gatagaaatt tagggttgga atttggccaa ttaccggaaa gcaaatgtac
      421 acaaaaattt tacataaata cgacaaatta tttccataca atcttgtttc catctgctat
      481 atactattaa gcagtttata aacagtttaa gttgctctaa ttaattataa atcggttatg
      541 cagcagtaaa agagggtgta tttaaaactt ataaagaaat atattccaga aactagctat
      601 gagcaaaatc aaactcgtaa aataatatga atcaaggaat tgtgattatt ttgctaaaac
      661 actggttttg ctcttccttc cctcagagta taaggctcta tagtaccttc actaatgttt
      721 cttccaataa tttctttaat aatatgcacc cggtaaaaag ggtaaaagaa tatgatttga
      781 ttatatataa atatatacta caaataaaag aaaggactat gaaatatgga tttaaattta
      841 aaagacaaag ttgcaatagt aacaggtgtt tgtggtgtta aaggtgttgc cggggttgga
      901 gcggctataa caagagtttt tgctgaacaa ggcatcaatg tagtggctaa ctatattgtt
      961 gatggagatg atgtaaaaaa ttatgttgat gaactaaata aaaaacatgg cactaaatgt
     1021 atggctgtgt atggagatat aagcaaacct gaagatattg ataatataat tgaaaaagca
     1081 attcaggcat atgggaaaat agacatactt gttaataacg ctgcaattct acctgttaca
     1141 aatattattg atatgccgga cgaagagtgg gagagagtta ttaagattaa tcttaatggg
     1201 acattctttt tctgtaaaag gtttgccaag cagcttatta agctaaatag gccgggcaga
     1261 atagtcaata ttacttcaaa gtcggggttt tttgtaactt ctcccggacg ctctcattat
     1321 gctacttcaa aaggaggaat aattaccctt acaaaagcat tggcgcgaga atttacgcaa
     1381 tatggaatta tagttaatgc aattgctccg ggcatattaa gaacaccact taacaatgag
     1441 ttacttgaag ataaggaaat tgagaggaat tatgtaaaaa ggataccctt gggcaggttg
     1501 acggatcctg aggaaattgc aaatatagta agctttttgg tatcggaaaa atctgaatcg
     1561 atagttggaa ctactattga tgcaactggc ggcatgctta tttagctcaa aagaagctga
     1621 tcaaaagaaa gcaaattaag aacataatga ccaacggagg tatgtctatg gctattatcg
     1681 ttaagaaaca aacttcaggt aataagaaaa atattgataa gaaagatatg gaacagcata
     1741 tttattggca agctcaccaa agcacaagta ctgttatgcc agaaaacacc atggctgctt
     1801 ttatgtacgc ctgggagctg gggggaatcc ctgaggcaga tattcgtaca accagtgatg
     1861 gagtaattat ttgcatgcat gactctactc cggcaagaac aacagatgct ccagattcgc
     1921 agagaagtct accagtatct tccttcactt tggagcaggt taaaaagtgg gatgcaggaa
     1981 taaaaacagg cgaattgtat agaggtgaaa tggtaccggc gttaaaagaa gcatttgagg
     2041 ttatgaaaca gaaaaaggat agattgctgt accttgattt aaaagaggtt gaccttggtt
     2101 ctcttggtaa actaattaat gagtatggtg ttaataatca aattattttc acgcattata
     2161 atgaagataa ttgcaggcaa atgtacaaaa cagttgaaga tatcaaaact atgctttgga
     2221 tagggggaac tcccgataag attaagggaa aatttaacaa ggtattatca gagaattttt
     2281 atggactaaa tcaaattcaa atccatttat atagcagaaa agacaataaa aaagacaata
     2341 aaaaagatag taataaagat agtaatgaag ataacaataa tgatagtaat aaaggtatta
     2401 ataaagttaa taatgataaa gttattggca tagattatga taataaaaat tccaatatgg
     2461 ctttgacatg tgaagaatgg ccatatgaga ttgataaagc ctttttggaa tatgcttatg
     2521 ataaaactct cacagcgggt ctggacttgg aggttctgcc ttttgaattt gacgacagct
     2581 caatacatac catactaaat ataggtatca gatggtttgc agtagatttc ccggaaaggt
     2641 tcataagttc tgtgaaaaag tgggaagata agttaacttc aaagtaacat caatattagt
     2701 gattatgcgt tagaagaaat aactgaaata cagaaggcag gtattatttc tggtacaggc
     2761 aaaggaagat ttgagcctaa agctaatgca acaagagcag aagctgcaaa aataatatat
     2821 ctgttgttta aagaattata agaattagca attagctaag ttatttaata gcattttagc
     2881 tgaggcatat atcatttgac agaaagcacc tcctgtattc acttttttct ttgcagttca
     2941 gctggaatat gtggtataat ataatctgca aaagcttaga ggcgttacct gatgattcca
     3001 aactggaggt gttataatgt caggacattc gaaatggtca aatattaaac ggaaaaaaga
     3061 gaaaactgat gcgcagaagg gtaaaatatt tacaaagctt ggcagagaat tagctattgc
     3121 tgtaaaagaa ggtggatccg accctgaagc aaatactaaa ttacaagatg ttattgcgaa
     3181 ggccaaagct gccaatatgc ctaatgaaaa tatcatgagg agtataaaaa aagcatctgg
     3241 ggatatggat gctgacaatt acgaggaaat atcttatgaa ggttatggtc ctggtggaat
     3301 tgcaattata gttgaggcaa tgacagataa tcgaaatagg acagcaggtg atataagaca
     3361 ctttttcgat aaattcggtg gtaaccttgg tcagaccggc tgtgtatctt ttatgttcga
     3421 taaaaaggga gttatattaa tcgaaagaac agatgatgtt gatgaagata cgcttatgat
     3481 ggaagttatt gatctgggtg ctgatgattt taatcctcag gatgaatatt ttgagatatt
     3541 gactgacccg ggagaatttt ctgccataag aggagccctt gagaaaaaag gctacaaatt
     3601 catggaagca gatgttgaga tggtaccgca gactgtagcg gtgttgacag atcctaaaca
     3661 tattgagcag atgaacaggc tgatagagaa tcttgatgac ctggatgatg ttcagaatgt
     3721 gtaccataac tgggaagagg atgagtaata aatcagggaa acagaaacaa ggggacggtt
     3781 cttctgtttc attcttttta aggagtgaaa ccaaagaatc gtccccttgt ttaattgttt
     3841 cattagcccg gggtcaagaa ctacctttga ctgcccgggc tatcagtact gtttgtcaag
     3901 cagccttcat ggccgccttg accgggacaa tcaatttata ttactctata ttcagtttaa
     3961 tctaaaaggg attttcggaa gaatttccgg aatttttgga gataaccctt ttgataaact
     4021 tcttatattc cattattgct tgttaacatt ataattgttc ccggtataag attcgaatac
     4081 tttcttaaca atgtttccac caattttacc tgcatctctt gcagcaatat caccgttgta
     4141 accctgtttc aagttaacac caacctggtt tgctatttca tacttcatgt tttcaaatgc
     4201 agttttgctt ctgttattag ccattaagac aacctccttt gattattatt tttattcgct
     4261 atttatcata gcgtattctt atcatttgta atttttcaaa aattattacg ggaattattg
     4321 tcaactataa atttttgttt accggattat tttagtcaaa tttattgtaa atttaactgg
     4381 caataaatct ggcaataaaa gggtccattt tttaacatat tagataattt agatttttct
     4441 atgttaaaaa gtttaaaact aaaaattatt ctgttgcagt attaacttct ctaaataaca
     4501 gactaatact ataaagaccg gcaaggccta cgagagtata aacagttttg ctgaggaatg
     4561 ttgtgttgcc cccaaaaatt gctccaacaa gatcaaaact gaaaagacca acaagaagcc
     4621 agttcaaagc acctataata acaagcacaa gagcaactct gtctatcggt gtcctattca
     4681 tgcaagcacc tcctttacta tttttatcta cagtatttcc aaactaagtt tttaaatgca
     4741 tgcaaatgtt tatatagcta gatagaatta caaaaatagg aaataaacag aaaagtgatg
     4801 tgtttaaaat aaaaactgcc gcgccttttt taagaggcgc ggtttatctg aataactacc
     4861 ggattccgcg gaggccggta gttagtattg ttcatataaa tatattacct gtttcccgtt
     4921 atatcgccgg tatcttccct attatactat gtatatggag taaatttcgt tcctcccgct
     4981 tctgataaat aaaaaaacac aatgtgatag aaaataatgt atttagtaat atccgggcaa
     5041 tatagtaact tggaacttat tgatacatat aatagtatga ggtcatatat gaagtaatat
     5101 ccatatgtca tggtaaattt tgcatacctg acatactgca tacaacaaat tatgaagaca
     5161 tatactacac ttgaagggaa gtgaagttga tgcagtttct cagcattgat gtagaaaaat
     5221 gtgctgatga tgttttagag tttatatcaa aagagttaat acagttgaaa aacgataaga
     5281 tagattactc aatagataga gttaataatg aaggaattga ctcaataata tgcaaattaa
     5341 ttgaaaataa gtctcaggtt aaagcaccta aacaagctta taaagcatta atgcttaatg
     5401 tatcaaatgc tcttgcagac tatattatac gatactatga agaaaaactt atcagacgca
     5461 ttgttaactc aaactattgc tattttagtc aaactgaaag aaaagatatt ttgaaggagg
     5521 cattcctaac tgtaaggaat gaagaaaaga gttttttaaa tactttactg cagattagaa
     5581 ggcggaatat tattgttaaa aatttaatgg aatactttga aaattcaaat aatgtaatat
     5641 tggatgggtt tgtaaatttc cggcttaaag attatataag ggatttggag gagattgtgg
     5701 agaaagctgt agatagcttt ttaatggaaa aagagtataa agaatttata aaattattaa
     5761 aatatttcgt tgatatacag gaacctaaat ttgacaatat acatgtaatt gcagaaagtg
     5821 aaggcaaata taatttactt gatgatagat atgaagaaat tacaaatgaa tgtataaagg
     5881 agtacctgaa tgaaatatca gaaggagaaa ttaattatga cgatttgctg gttagctcat
     5941 tgataacatt agcacctaaa aaaattacaa ttcacagaac agcacatttt aaaaataaag
     6001 aattactgga aacaataagg aatatttttc aaaagaatgt agtaatttgt aaaggctgtg
     6061 gtatatgcaa tgcaacttca gcaactaata aaaaaagtat aaaggaagca gaaattccgg
     6121 ataatatata ttgacatatt tattataaat aatgtaacat atggtataag tagatataaa
     6181 atgaacttta aggtaaatga agaagagagt ttttaactct ctttattcta tattatcaca
     6241 gaaatgcact ttccattagc gacataacaa agtagtacta atagtgtatc ctttaagttt
     6301 cttctcatga aatatttatg gatctatata agtttcttca catttgtggt aaaattacat
     6361 tgtaataaat actggttgtg tttaaaaaat tgattttata taactataag taattaatgg
     6421 aggtaaatta ggttgagggt agtgggcttt gtaggtgcca gcggtacggg aaagagtcat
     6481 agagcgttac aggtggccag tagtagagga attgaatata taattgatga tggccttcta
     6541 ataaagggaa atagcgtaat agcaggagtt tctgcaaaaa aggaaagcac taaaataggc
     6601 tcaattaaaa gggcattatt tatttatgat gaccatgcaa agagtgtaaa gaaagctata
     6661 gagcttgcta atcctaatgc tatcctgata ttgggcacat cagatggtat ggtggaaagt
     6721 atcgccaaaa agctggacct tcctgaagta tcacaaaaag tatatataca tgaagttgca
     6781 aaggaagctg agatcaatca ggcgctatct acaagaagaa atgaaggtaa gcatgttatt
     6841 ccagttccta cgtttgaaat taaaaaggac ttttcaggct attttcttga tccgctgcaa
     6901 atattcagga gaaaagggaa gggacgtttc caacttgttg cagaaaaatc tgtggtaagg
     6961 cctacattta gctatatggg caaatacact atatccgact ataccatata ccagatagct
     7021 gaatatgtta tatatgacat aaaaggcatt agcaggatat caaggtttaa agtggaaaat
     7081 ggcaacgaag gtataaccat tgacatagat ttaatactta tatatggtta tcctattaaa
     7141 tctctgctgg aaactgttca gcaaagagtc aagctggaga tagagaaaat aactgccttg
     7201 aatataaagg ccttaaattt gactgcaaaa gggctatata ttcaagaaaa ttcgcttagc
     7261 tcatgactta agcaatgatg cgaattttct tttctgacct tcaattatca actatcaaat
     7321 atcaaatatc aaccatcagc tgtcaaccat cagccgtcaa ccatcagctg tcagctttca
     7381 actctcaacc gtaagtcttc aacctcccac tacccaatat ccactaccca ctgctcacta
     7441 cccacctttc acttgagatt gcagtaacct gtgatttttt gtatgcttcg ggggagagca
     7501 tcttttgagt tgccccacgg ggcatcgtta ttacggtaat caaacttctc cgtaaaccaa
     7561 ttaacttcct gttgaaccct ttccatgttt ttcaactgca ttttcaaaag attatcagca
     7621 aatattgcca tatcccgaga atttaggtat ttacccatac tttcaagaag ttgtctgaaa
     7681 tgtacaagac aaaaaccttt attgttgtta aacaagtccc ggaactctct ttctttgaac
     7741 cataaataga atattacgtc aatatatctt tccattgtat agctcatttt tccgcaaatc
     7801 atgcactttt tttcaaggct ggccagctct tgaaccatat tctctacaaa tttttctgtt
     7861 gatgtctttt tagaagtaag cttatttgat atgcttttaa ccagggtagt ttctgactct
     7921 ttttggatat aactaatttt tttgctgtac attttcttca gttccttatt ttgctcctga
     7981 aggtgagtat caacaatgag tcctagacct aatctgtttt cctgcctgtt ataaagcaat
     8041 tcaaaatgcc tcgtgcaaaa tcccttttca tttgtctcta ttctgcaatc aggctccata
     8101 agtgacggcc caagaaagta ttcaatatac tggtcctcaa gctttctttc aagtgtacat
     8161 atggcacatt cactatcttc attaaatgca tcaacaacag gtatagtgta gatttgttct
     8221 ttcatatttt aaaattctcc attatactat atttatataa atgaatatat gcatattata
     8281 gcagaaaata ggtataatgg gaaggcgata taagattagg gggaattttt cttggagtgc
     8341 gaagaacata caatggaata taaaggatat aaaatacaag atcaccaaag ctccggcggc
     8401 gggaagctca taagggttta tgatgtaagg gattttaatc ctgtacacat ttttgaatgc
     8461 gggcaatgtt ttcgctggta cagggaacac gacggaagtt atacaggtgt tgcaaaaggt
     8521 aaagttgcta atatatttta tgatgcttat gattattgtt cggaacataa tgatgggcat
     8581 ggcagtgata aaggaggtac tctttatata agaaatgccg acatggaaga ttttaaaaat
     8641 gtctggtatg attatttaga cttgggcagg aattatggtg atataaaaga agcgctcatg
     8701 cgtaaagatg atataatgaa gaaagctgta gagttcggct atggtataag gctgttaagg
     8761 caggatttct gggagacatt aatatcattc ataatatctg ccaataacag aatacccatg
     8821 ataatgaaag ttgttgcttc catatctgaa tcctttggta aaaaaatatt gattaacgaa
     8881 aaagcatacc atacattccc ggatgttgaa aatcttgcga gtgcatctat tgaacaactg
     8941 gcagtatgcc gagccgggtt tagatgcaaa tatattgata ttacatcaaa aatggtggag
     9001 gaaggctgcc tgaaaccttc agaattatat cttgtgaata ctgatgaagc cagggaaatg
     9061 ttaaaacagt tgcctggtgt tggtgataag gtagctgatt gtacattatt atacagtgga
     9121 attaaatatg atgtttttcc tactgatgta tgggttaaaa gagttatgga ggaactatat
     9181 tttcacaggg aagcgactct aaaagaaatt cagaaatttg ccgcgaatta ctttggagag
     9241 cttgcaggct ttgcgcaaca atatcttttc tattatgcaa gagaaaataa aattggtgcc
     9301 aaataacttg taacggaaaa gaaaatgcaa aatagtatga tactgacaga cttaagcatt
     9361 tatctgttaa aaattaggag gggtgtatat gtactatctt gtgttagtag attccgggaa
     9421 ttatgccata aggctttcca ggattatggc tcaaaaagga tatcctgttg aagtagtttc
     9481 tacaccgtgc aaaattgcaa agaatggttg tggttactgt ctaaggttaa ttcctgaata
     9541 taaagataca cttattaatg aaggaaagac aaatgctatt aatatcagag aaatttatag
     9601 tgtaaccaat gcctcttata aaaacattta taaaaagatt tatccttaag ttattggggg
     9661 tattatgtta aaatccagta ttatacaaag gtaactagta aatatttttg attttcgaag
     9721 tattattgag taaatggcca aaatagagtg tgcggaaggg gtaaacaggt ttaaaatttg
     9781 ttgttaagct ttcatatgaa tgatataata tcctcataaa tgaggatatt atatgcctcc
     9841 ggcggaattt atgt
//
LOCUS       DAOSDD010000174         9769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2613_length_9769_cov_5.279391, whole genome shotgun sequence.
ACCESSION   DAOSDD010000174 DAOSDD010000000
VERSION     DAOSDD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_9769_cov_5.279391"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PK733_13900"
     CDS             complement(<1..429)
                     /locus_tag="PK733_13900"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOJ11671.1"
                     /translation="MHITDNPKDKISTVQAIVFISSLTVGAAIITLPRSAARSVNTPD
                     VWASILFGGLIAIIPSIILVKLSQRFPGKTCFQFNRIITGKFLGWILSFLMIAYYTLL
                     AGFEGRMMVELVRVYLLPRTPIEVALMAFLWAGTYLTVSGI"
     gene            complement(435..2060)
                     /locus_tag="PK733_13905"
     CDS             complement(435..2060)
                     /locus_tag="PK733_13905"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ11672.1"
                     /translation="MFKRQKQRRELQDKGNTSKNASKNTSENVSKNADKNTSKITGNI
                     DEDIKTIKAALCCSPDLVIRRLKPADSSDDTVVAYAVMYIEGLADRKVIENNVIKPLL
                     KELSQEAKSSSDKAYTTRTSITEKIKNTIASVSNLNETDNFDECINEVLTGNTIFLTS
                     KSKSALVFNTPGWKTRNPEEPISEPSVRGSRDGFVETLQENIVRIRRRVRDPGFAIKK
                     LKVGRRTKTDVAVVYISNIANKDLVEEVVHRIEQINIDEMISAGTLEQFIEDNFLSLF
                     PQIQYTERPDRVVAALMEGRTAIILDNTPFVLIVPATLPMFIQSPEDYYDRWIYSSVI
                     RVLRYLVVLFSLFLPSVYIAYISFHQGLIPTKLAIFIASTREGVPLPSLIEALLMETT
                     LEILREAGIRLPKTIGQAVGIVGGLVIGEAAVRAGIVSPVSVVVVALTAIASFSVPQY
                     AIGIPIRLFRFVLMITAGILGLYGVMLAFIMITVHIVRLKSFGVNYMSPFVPYRPGDW
                     KDFIFRLPVMMMKKRPEILKTEDQWRQGNSGKG"
     gene            complement(2137..2268)
                     /locus_tag="PK733_13910"
     CDS             complement(2137..2268)
                     /locus_tag="PK733_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11673.1"
                     /translation="MNRTLDRMLVFFFDLKLWLGAFTAFAVPLGFSFLFKWIREKSS"
     gene            complement(2359..3276)
                     /locus_tag="PK733_13915"
     CDS             complement(2359..3276)
                     /locus_tag="PK733_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11674.1"
                     /translation="MGVTMKKENAGNNKFHFDRTYYNEPQIFDFITLYQIGDLYCQGG
                     YSIGEHKQYCYEISYIASGKGYYSSDDKKYPVKTGDIYLNRPGELHNGLADPIDPFRY
                     FYAGFTFINDNVEQNPFTHIKKMFDQLKRPVVQDQLDVKTKFLNIFNELINLKEYSSL
                     MIKMYLNQIILLTYRNFFDQWENQYIPDKDAANQKSMVYLIVNYIDKNLCKIKDLTQI
                     ADELGYSYSYISHIFSQETGLSIMEYYNQKRFEKAGELLKNGSLSITEIAEMLQYQSI
                     NSFSKAFRKNIGISPSEYQALYSKEDPRL"
     gene            complement(3358..3981)
                     /locus_tag="PK733_13920"
     CDS             complement(3358..3981)
                     /locus_tag="PK733_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ11675.1"
                     /translation="MDNIHAKSTKKIVLNGVMAALVFLATFFTRIPGPVPPGYINFGD
                     AVIMITAILLGKSSGFIAGAFGSALADVAAPGGLLFAPITFIVKGIEGYVIGAIARSR
                     TTDEKHANGAAIQASEVSEDSHKNKSPGYYEVRKVIAVIAGALIMVAGYFIAELSILK
                     LFDSTFGYTAAISELPFNFIQGGVSVVLGYVLSSLLEKAGVHRLLEV"
     gene            complement(4148..5437)
                     /locus_tag="PK733_13925"
     CDS             complement(4148..5437)
                     /locus_tag="PK733_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11676.1"
                     /translation="MNVPSDISSDKSSSVSSDIQLNNLPHILEISELDYFIENIQILK
                     NINFQAGKGEFIGLIGPNGAGKTTLLKCINGINKGNGQVFINKKTLERLSNKRIAREV
                     ALMHQNTSVSFPFPSIDVVLAGRYPYLKFASGETIDDFRIARKYMEYTGTSQFEKKPI
                     TTVSGGERQRIFFAKILTQETDIILLDEPTSNLDIAYEEQIFRYCNDLCNNGKTVIAA
                     VHDLKIASRYCSRLILMKEGEIIADGKVDEVLTSGNLSEAYGVNALVYRNRVTGLLDF
                     YIPGFEHRKEYRHVHIIGGGGSASGVIRQLFERGFCLSTGVLSYGDSDLNCAEVFEIK
                     RVVNKPFSGISDEAFNENIKYIRESDITILCNMPFGIYNIRNLDAARYASNLVIIEDD
                     SPEVRDFTEGKALEIYKELKKNAIVTTYARLHEVIVT"
     gene            complement(5434..6462)
                     /locus_tag="PK733_13930"
     CDS             complement(5434..6462)
                     /locus_tag="PK733_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ11677.1"
                     /translation="MYYKLKSVLPVMLAVLAAMIILATAVGAVSVPFIETIKIILKNW
                     GFLKHLTFTEGWESIIFYVRFPRVITTVLVGAALGASGAVMQGMFRNPMADPGILGVS
                     SGASLGAVIAISLGIPSKGIYFMPIFASIGALLAAFTVFILSSRDGKIPVMNMILSGI
                     AVSMFLGAITTGILSFIRSDQVKQFLFWTMGSLNGKRWEEVRIAAFPILVCISILLFF
                     SRDINVLLLGEEEAQSVGLNPSRTRKLLLLFSSILTASAVSVSGTISFVGLIVPHIIR
                     LLIGPDHRVLIPASALGGAIFLLVCDLIGRTIMAPGEVGVGIVTSIIGAPYFLYLLSK
                     ARKEGTAL"
     gene            complement(6501..7592)
                     /locus_tag="PK733_13935"
     CDS             complement(6501..7592)
                     /locus_tag="PK733_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ11678.1"
                     /translation="MSKIQKKPNKWDKNILKVLSLIVITVMLLTVFSACTIKEANKTA
                     DQNASLQLGQSGTVQNNEKETKTSNNNETGKENEGFPLKIKDAKGTEVTIEKKPEKIV
                     SLTLTTDEMLLSLVDDNRIAAISHLSVDPGLSNIAEEAAKIPVKAKLELESLIALQPD
                     LIIVSDWTDDNAVKQLRDANITVYAMATSNNIDEVKDAVLEVAKLVGEEQNGHEIVSW
                     MNEKLKVVDEKIKTLKDDQKAKALIYDSFSTTYGKGTTFDSIAKNAGIINLASEAGIE
                     MYQTIEKENIIKMNPDVIFLPSWSFEGFDAKKFTEDFKNDKSLAELKAVKNNKVFNLP
                     DKHMVTTSQYIVLGVEDVAKAVYPDLFNK"
     gene            complement(7623..8150)
                     /locus_tag="PK733_13940"
     CDS             complement(7623..8150)
                     /locus_tag="PK733_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOJ11679.1"
                     /translation="MCGLIHIYTGDGKGKTTAAIGLGVRACGRGMKVLMVQFLKGIET
                     GEMIVLKTLEPNFVLYRSRQLKKFTWEMSAEEQKGASKTQQDLLEYAKKAAFNENIDL
                     LILDEIMAAINTGFLDKEIVLNFIKNKPHNLELVLTGRNAPKEFTEIADYVSEIIAVK
                     HPMNEGISGRKGIEF"
     regulatory      complement(8276..8505)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8645..9727
                     /locus_tag="PK733_13945"
     CDS             8645..9727
                     /locus_tag="PK733_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11680.1"
                     /translation="MKTVRLGIIGTGMAWERLHYPAIQELGDKYQIVALANRTREDAE
                     KFARKINLPLDNVYDDYNEMLKRNDLDAVDILVPIEINYEVSEDVARAGKNFICEKPL
                     APDMKQAKKFLDLSKKYKVKIMIAENYRYNEENNKIRDLVNSGKIGDVIYFIKNNITC
                     FPCEMTKDTFAATEWRQHPDFEGGAFLDAALHDLAAVRHIFGAVKYVSAFGKPQKEDY
                     NPYISINTNIMFQNGIIGQYTYFPSGQEPQRPLVGFRIFGTKGEIYLEEKNCGIINVS
                     YNDGNAEQIAFTPERGYYNELLNFYNAMNGTEEISVTPQIEYGDVKMVFDILESISKK
                     KVIEVDEPRKLEADLSYYKETRSYIQ"
BASE COUNT         3033 a         1992 c         1580 g         3164 t
ORIGIN      
        1 tattccacta acagtcagat aagttccagc ccaaaggaat gccatcagcg ctacttctat
       61 gggcgttctt ggtaaaagat agacacggac aagttccacc atcattcgtc cctcaaatcc
      121 tgccaataga gtataatatg caatcatcag gaagctcagt atccatccca ggaactttcc
      181 tgttataatc ctattaaact gaaaacaagt ctttcccgga aacctctggc ttagctttac
      241 caggataatg cttggaataa ttgctattaa tccaccaaaa agtatggatg cccaaacatc
      301 aggggtgttt acgcttctag ctgcggacct tggtagtgtt ataattgcag caccaaccgt
      361 taatgatgaa ataaaaacta ttgcttgaac tgttgatatt ttatctttag gattgtcagt
      421 tatatgcatt caccttaccc tttcccggag tttccctgcc gccattgatc ttctgtcttt
      481 aatatttctg ggcgcttctt catcatcatg acaggaagac gaaatataaa atccttccaa
      541 tcccctggcc ggtatggcac aaaaggagac atatagttaa ccccaaaact tttaagcctg
      601 acaatatgca cagtaatcat gataaaagca agcatgacac catataatcc cagtattcct
      661 gctgttatca tcaagacaaa cctgaagaga cgtatcggaa tgcctatagc gtattgaggt
      721 acagaaaaag atgcaatagc tgtcagggct acaacaacta ccgatacagg acttacaatc
      781 cctgctctca ccgcagcctc acctataaca agacctccta caattcccac cgcctgacct
      841 atagtctttg gcagacgtat accggcttcc ctaagtattt caagagttgt ttccattaaa
      901 agagcttcta ttaatgaagg aagaggaacc ccttctctag tagaagcaat aaaaattgca
      961 agctttgtag gtataagccc ctggtgaaaa gatatataag ctatatatac agaaggtaga
     1021 aacaaggaaa ataaaaccac caaatacctt aaaacacgta taactgaact atatatccat
     1081 cggtcataat aatcctcagg tgattgtatg aacataggaa gagtagctgg aacaataaga
     1141 acaaaaggag tattatctaa aataattgct gttcggccct ccattaacgc cgctactacc
     1201 ctgtcggggc gttctgtata ctgtatctgt ggaaacaaag acaaaaaatt atcttcaata
     1261 aattgctcca aggttccggc tgatatcatt tcatcaatat ttatttgctc tatgcgatgt
     1321 actacttcct caacaagatc cttgttggca atattactta tatatactac agcaacatca
     1381 gtttttgtac ggcgtcccac ttttaatttt tttatggcga aaccaggatc gcgtactcgc
     1441 cgtcgaattc taacaatatt ctcctggaga gtttcaacaa agccatctct tgaacccctt
     1501 acactgggct cagatattgg ttcctcaggg tttcttgttt tccagccggg tgtattaaat
     1561 acaagtgcac ttttactttt ggaggtcaaa aagatggtat tccctgttag cacctcatta
     1621 atacactcat caaaattatc tgtttcattc aggttactaa cactggcaat ggtatttttt
     1681 atcttttcgg taattgatgt tcttgtagta tatgctttat cagatgaact ttttgcttct
     1741 tgtgaaagct cttttagtaa tggttttatt acattattct ctataacctt tctatcagct
     1801 aatccttcaa tatacattac tgcatatgcc actaccgtat catcactgga atcagcgggc
     1861 ttcagtcttc gtataaccag gtcgggactg caacataggg cagctttaat agtcttaata
     1921 tcttcatcaa tatttcctgt gattttgctg gtatttttat cagcattttt gctgacattt
     1981 tcactcgtat ttttactggc atttttactc gtatttccct tatcttgaag ctctcttctc
     2041 tgtttttgtc tcttaaacac tattattcct ctctttcccg tatttttatt atgttcagaa
     2101 ggaaagaaat aatacctgtt tgttgaggca aaaatcttaa cttgattttt ctctaatcca
     2161 tttaaatagg aatgaaaatc cgagaggaac tgcaaaagct gtaaaggctc ccaaccacag
     2221 tttcaagtca aaaaaaaata caagcatcct atcaagtgtc ctgttcataa atatgagagc
     2281 acctcccctg tatccaaaat taatttaccc atatatagat aggattatcc ttaatactac
     2341 tatgccgatt tgattaagtt ataacctggg atcctctttt gaatacagtg cttgatattc
     2401 tgacggcgat atgcctatat ttttacggaa ggctttgcta aaggagttaa tagattgata
     2461 ttgaagcatt tcagcaattt ctgtgatact caagctgcca tttttcaaaa gctctcctgc
     2521 tttctcaaac cgcttttgat tgtaatattc cataatacta agacctgttt cctgtgaaaa
     2581 aatgtgggaa atatatgaat aactgtatcc cagttcatcg gcaatctgtg tcaaatcttt
     2641 tatcttgcac aaattcttat caatataatt tactatcaaa tataccatac ttttttgatt
     2701 agctgcatct ttatcaggta tatattgatt ttcccactgg tcgaaaaaat tcctgtatgt
     2761 taataatatg atttgattaa ggtacatttt tatcattaaa gacgaatatt ctttcagatt
     2821 aattaactca ttaaatatgt ttagaaattt tgttttcaca tccagttgat cctgaacgac
     2881 agggcgtttc aactggtcaa acatcttttt tatatgagta aaagggttct gttccacatt
     2941 atcattaata aatgtaaacc cagcataaaa atatctaaag ggatctatgg ggtctgccag
     3001 gccgttatga agttcaccgg gcctattaag gtatatatct cctgttttaa ccggatattt
     3061 tttatcatca ctggagtaat atcctttacc agatgcaata tagctgattt catagcaata
     3121 ctgcttatgt tctccaatgg aataaccacc ctgacagtac aaatccccaa tttgatatag
     3181 agttatgaaa tcaaatatct gaggttcatt ataatatgtc ctgtcaaagt gaaacttgtt
     3241 attccctgca ttttcttttt tcatagttac acccatttat atatcaactc ccatagatgg
     3301 gaaggggaat ccccttccca ggcccagtat aggaatcttt aggaatttta tgaattccta
     3361 tacttccagc aacctgtgca cgccggcctt ttccagcagt gatgaaagaa catatcctaa
     3421 aactacactt acaccaccct gtataaaatt gaatggtagt tccgagatgg cggctgtata
     3481 cccaaaagtt gaatcgaaca atttaagtat ggataattcc gcaataaaat aacctgctac
     3541 cattatcaga gctcctgcta tcactgcaat aaccttcctt acctcatagt atccggggct
     3601 tttgttttta tgagaatcct ctgaaacttc tgaagcttgg attgcagctc cattagcatg
     3661 cttttcatca gttgttctgc tgcgggcaat tgccccaata acatatccct caataccttt
     3721 tactataaaa gttataggag caaataaaag tccccccggg gctgctacat cagccagtgc
     3781 ggaaccaaaa gcacctgcga tgaaaccgct gctctttcct aataaaatag cagtaatcat
     3841 tataactgca tcaccgaagt taatgtatcc gggtggaacc ggtccgggaa tacgggtaaa
     3901 aaatgtagca agaaaaacca atgctgccat aaccccattt aaaactattt ttttagtact
     3961 ttttgcatgt atattgtcca ttaatctatt tcacccctgt aatatataac agcaatacag
     4021 atctgtctgt attctttact gtgcttctta tagcagtact attttatatt gctttttaat
     4081 tattataaca catttataac acatataaat aatttttatt aaatacaagc acatattagg
     4141 gtgcatatta tgttactata acttcatgca accttgcata tgttgttaca attgcattct
     4201 tttttaactc tttgtatatt tcaagtgctt tcccttcagt aaaatccctt acttcaggac
     4261 tatcatcttc gattattacc aaattggaag catatcttgc agcatcaagg tttcttatat
     4321 tataaattcc gaatggcata ttacaaagta ttgtaatatc agattccctt atatatttga
     4381 tattttcgtt aaaggcctca tcactaattc cactgaaagg tttgttcact acccgcttta
     4441 tttcgaaaac ctcagcacag tttaaatcac tgtctccata tgaaaggaca cctgtagaaa
     4501 ggcagaaccc tctttcaaag agctgtctta ttacaccgga agcagaaccc ccgcctccaa
     4561 taatgtggac atgcctgtat tcttttctat gttcaaaacc cggtatataa aaatccagga
     4621 gcccggtaac tctgttcctg taaacaagcg cattaactcc ataggcttct gacaggttac
     4681 cggaagtcaa gacttcatca acctttccat cagcgataat ttcaccttcc ttcataagga
     4741 taagccttga acaatatctt gaagctattt tcaagtcatg gactgctgca ataaccgttt
     4801 tgccattatt acacaaatca ttgcaataac ggaatatctg ctcttcataa gctatatcaa
     4861 gattagaggt aggttcatca agcaaaatta tatcagtttc ctgagtaagt attttagcaa
     4921 agaatatccg ctgtctttca ccacctgaca ctgtagttat tggttttttt tcaaactggg
     4981 aagtgcctgt gtattccata tatttccttg caatacggaa gtcgtctata gtttccccgg
     5041 aagcaaattt taagtatgga tatctgcctg caagcacaac atcaattgag ggaaaaggaa
     5101 agctcacgct ggtattttgg tgcattaatg caacctccct ggcaattctt ttattgctga
     5161 gtctttcaag agtcttcttg ttaataaata cctgtccatt ccccttgttt attccattta
     5221 tacacttaag aagagtggtc ttacctgcac cgttaggacc tataagtcct ataaattcac
     5281 ctttacccgc ctgaaaattt atatttttta atatttgaat attttctata aaataatcga
     5341 gctctgatat ttcaagtata tgtggcaggt tgtttaattg tatatcagat gatacactag
     5401 atgatttatc agacgatata tcagatggta cattcataaa gcagtacctt cctttcttgc
     5461 cttgcttagc aggtaaagaa aatatggagc tccaataatt gaggtaacaa ttcccacccc
     5521 tacttccccg ggagccatta tggtcctgcc tataagatca caaacaagaa gaaatatagc
     5581 tccaccaagt gcacttgccg ggataagtac tctatggtca ggccctataa gcagacgaat
     5641 tatatgggga actattaatc ccacgaaact tatagttccg cttacgctta ctgccgatgc
     5701 tgtgagtatg gaggagaaga gcagcagcag ttttcttgtt cttgaagggt ttaaacccac
     5761 agactgagct tcttcttccc caagtaacag aacattaata tcccgtgaaa agaaaagcag
     5821 aatagaaata caaactaata ttggaaaagc agctatcctc acttcctccc acctcttccc
     5881 attcaggctc cccattgtcc agaaaagaaa ttgttttacc tggtcactcc tgataaatga
     5941 aaggattccc gttgttatgg ctccaagaaa catacttacg gcaattcctg aaagtatcat
     6001 gttcataaca ggtattttac catcacgtga agacagtata aaaactgtaa aagcggccag
     6061 caatgcccct atagaagcaa aaatgggcat aaagtatatg ccttttgaag ggataccaag
     6121 agaaatagct ataacagcac caagacttgc tccgcttgat actcctaata ttccggggtc
     6181 tgccataggg tttctgaaca ttccctgcat aacagcccct gaagctccca gagctgctcc
     6241 taccaataca gtagttataa ctctgggaaa cctgacataa aatattatag attcccatcc
     6301 ctcagtaaag gtaagatgct taaggaatcc ccaatttttt agaattatct ttattgtttc
     6361 aataaaagga acactcacag cccccactgc tgttgccaat ataatcatcg cagcaagtac
     6421 tgcaagcatc acaggtaaaa ctgattttaa cttataatac atatgcaccc tccccgctgc
     6481 accgttgctt cactgttgca ctacttgttg aacaaatcag gatatactgc ctttgctaca
     6541 tcttccacac ccaggacaat atactgggaa gttgtaacca tatgcttatc aggaagatta
     6601 aataccttat tattctttac tgcttttaat tctgcaaggc ttttatcgtt tttaaaatcc
     6661 tcagtaaact tttttgcatc aaagccttca aaagaccatg atgggagaaa aattacatcc
     6721 ggattcattt taatgatgtt ttctttttca attgtttgat acatttctat tcctgcttcc
     6781 gatgctaaat taataattcc ggcattttta gcaatactgt caaaagttgt gcctttccca
     6841 taggtggtag agaaagaatc gtagattaaa gctttagctt tctgatcatc ttttaatgtt
     6901 ttaatcttct catcaacaac tttcagcttt tcattcatcc atgaaacaat ttcatgccca
     6961 ttttgctctt cacctacaag ttttgccacc tctaaaactg catccttaac ctcatcaata
     7021 ttattagaag tagccatggc ataaactgta atattagcat ccctcaattg cttaactgca
     7081 ttatcgtcag tccagtctga tactattata agatcaggct gcaaagctat aaggctctca
     7141 agctccaatt ttgcttttac aggtattttt gcagcttcct ctgctatatt tgaaagaccc
     7201 ggatccactg aaaggtgtga aatggccgca attctgttgt catcaaccaa tgataaaagc
     7261 atttcatcag tagtaagtgt aagtgaaacg attttttccg gtttcttttc aattgttact
     7321 tcagttccct tggcgtcctt tattttaaga gggaagccct cattttcctt acctgtttca
     7381 ttattatttg atgtttttgt ttctttttca ttattttgaa ctgtcccaga ttgtccaagt
     7441 tgtaatgacg cattttggtc tgcagtctta ttagcttctt ttatagtaca tgctgaaaat
     7501 actgtcaata acataactgt tataactatt aaactcagaa cttttaaaat atttttgtcc
     7561 catttatttg gttttttttg tattttactc atttgcaaaa tcccccttaa ttaataactt
     7621 tactaaaatt ctataccttt ccttcctgaa ataccttcat tcataggatg ttttactgca
     7681 attatttcag atacataatc agcaatctca gtaaattctt ttggtgcatt tctacctgta
     7741 agcacaagtt caaggttatg cggtttattt tttataaaat tcaacactat ttctttatcc
     7801 agaaatccag tatttattgc tgccataatt tcatcaagga taagaagatc aatattttcg
     7861 ttaaaggccg ctttcttggc atattccaat agatcctgct gagtttttga agctcccttc
     7921 tgttcttctg cactcatttc ccaggtaaat ttttttaact gtctgcttct gtataagaca
     7981 aaattgggtt caagggtttt cagaacaatc atttcacccg tttcaattcc tttaaggaac
     8041 tgaaccataa gaactttcat tccccttccg caggctctta cacccagacc tattgctgca
     8101 gtagttttcc ctttaccatc accggtatat atatgtatca atccacacat ggctctccat
     8161 ccttaatttg tattagacag ctaaaaaaac ctcttaaccc atcaaaggtc aagaggtgtt
     8221 aaaaccatag catatcagtt ttttaatcaa tcacaccccc tatcgaccgt agagtatata
     8281 gtgtatcaat acaggcaggt cttctggctc aagaatcatc acccttttac ccttcccatc
     8341 ctgatgtcca taaatacttt ttgtttcatc aaccggacag tggtaatata aaaggattcc
     8401 tcttttacag cggcgggacc gcgcgggact ttcacccgtt tcccttttaa ttaatctata
     8461 taataagcaa aatatagata ataaacccgt attggtattt atttaattaa tataagttta
     8521 cagtatataa gaagaatatg caacgtgttt ttttgttgtt ttttatatgt tttgcataaa
     8581 taaagtttta tgaataataa tattttagga gcaaatataa ataaaatagg aggagagtga
     8641 ctgaatgaaa acggtacgat tgggtataat tggtacaggg atggcatggg aaaggcttca
     8701 ttatccagca atacaagaac tgggtgacaa atatcaaatt gttgcacttg caaaccgcac
     8761 aagagaagat gctgaaaagt ttgccagaaa gataaacctg ccgcttgata atgtatatga
     8821 cgactataat gaaatgctta aaagaaatga ccttgatgct gtcgatatac ttgttccaat
     8881 agaaataaat tatgaagtat ctgaagatgt agcaagagct ggtaaaaatt ttatttgtga
     8941 aaagcccttg gctccagaca tgaagcaggc taaaaagttc cttgatttat caaaaaaata
     9001 taaagtcaaa ataatgattg cagaaaacta cagatataat gaggaaaata ataaaattag
     9061 ggatttggta aatagcggca aaattgggga cgttatatac tttattaaaa acaacattac
     9121 ctgtttccca tgtgaaatga caaaagacac ttttgcagca actgaatgga gacaacatcc
     9181 tgattttgaa ggtggtgcat ttcttgatgc tgcattgcat gatttagctg cagtaagaca
     9241 catttttgga gcagtaaaat atgtcagtgc atttgggaaa cctcagaaag aagattataa
     9301 cccttacatt tcaataaaca cgaacattat gttccaaaat ggcatcatag gtcaatacac
     9361 ctacttccca tcaggccagg aaccccaaag gcctcttgta ggattcagaa tatttgggac
     9421 aaaaggcgaa atataccttg aagaaaagaa ctgtggaatt ataaatgttt cgtataatga
     9481 tggaaatgcc gagcaaattg catttactcc tgagagagga tattataacg agcttctgaa
     9541 tttctataat gcaatgaacg gtactgaaga aatatccgta actccgcaaa ttgaatatgg
     9601 agacgtaaaa atggttttcg atattctgga atcaatatca aaaaagaagg ttatcgaagt
     9661 ggatgaaccc cggaaacttg aagcagattt gagttattac aaggaaacaa gatcatatat
     9721 acaataaatc attatagtta tgcaatagct ttgcatatta taattcgtt
//
LOCUS       DAOSDD010000175         9756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2615_length_9756_cov_5.193382, whole genome shotgun sequence.
ACCESSION   DAOSDD010000175 DAOSDD010000000
VERSION     DAOSDD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2615_length_9756_cov_5.193382"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1035)
                     /gene="ftsY"
                     /locus_tag="PK733_13950"
     CDS             complement(121..1035)
                     /gene="ftsY"
                     /locus_tag="PK733_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOJ11681.1"
                     /translation="MGLFDKLKQGLEKTRKGITGTIDQILVSFGKIDEELFEELEEAL
                     IASDVGVDTTLKITEDIKKKVKERKIVNPKEVKGLLKEEIINILSEGDSRLNIQTTPS
                     IIIVVGVNGVGKTTTIGKIAANLKKNRKNVVLAAGDTFRAAAIDQLEIWSKRAGSELI
                     KHNEGSDPAAVVYDAIQAAKTRKADVLICDTAGRLHTKKNLMEELKKIFRVIDRELPD
                     ASQEVLLVLDATTGQNAVSQAKVFSEAVDISGIVLTKLDGTAKGGIVIAIKSELDIPV
                     KLVGVGEQIDDLQEFNPHDFAEALFYEE"
     gene            complement(1111..4683)
                     /gene="smc"
                     /locus_tag="PK733_13955"
     CDS             complement(1111..4683)
                     /gene="smc"
                     /locus_tag="PK733_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOJ11682.1"
                     /translation="MHLKRLDIQGFKSFSDRTTLDFDPGITSIVGPNGCGKSNIVDAI
                     RWVLGEQSIKTIRGSKLEDVIFTGTEFRKPVGFAEVALTIDNSEGDLPLEYSEVTISR
                     RFFRSGESEYYINKTPCRLKDINNLFLDTGVGKDGYSIIGQGRIDEILSTKSEDRRNI
                     FEEASGIMKYKVRKEEAEKKLNLTKQNLLRIDDILHELESRLEPLQEQASTARKYLSL
                     RERLKELDINVYLDLISKLKEKIAECDGHYNSVMESKNGLVHKYNLTSNDNENKSKYL
                     KNLEDKLDSCRKEYYALETSLEKNQGELNLNNEKISNLKQNTYRIDNEISNLSNRINK
                     ISSILADNDKKLEDFITVHDHLLDDIKKCEQKREEILLTLNTHEKHLETMNSTIMNKL
                     DLLSDKKIQVGNVNSHIESLCKQQQHIENEVRELDLNIAKEIQKKETLEKSIELKIQE
                     LNTGMSSLEALISSRKELDSRLSIEKEKKNSVSSDIQVKVSKQKMLKGMEDNLEGYNR
                     TVKAFLQACKKADSTGKGICGALGQLISVKKGYSTAIEMALGGAIQNIVAETEEDAKR
                     AIEFLKKNSMGRATFLPITSVKGNYLDSNILKEVKKCSGFCGVASDLVEYNSEYNSIV
                     LSFLGRVIVVRDLDSGIYMARKFNYSFRIVTLEGDILNTSGAMSGGSRDVNATGILGR
                     TGQISELEEDIHKLNKELIDFEENIKTIISNMEKLSKDISMMQGILKEREMSKLREEN
                     QLSNTNMNIKNITAITSMLKQEKEQLIRQQEETRVELHKYISEQEKIEEEISQLKNDI
                     EQYQETQRGNLSERDSIQSELTGYKIAINSAINDINIVKKEIEKLLEEKDAVSRNISD
                     KRNEKAKEERQIEWFNEKNIILSNAIKTCQEEKVGRNIEIDRMAEERKVIEEEVSNAS
                     TSMEDISRNISLLNEDLNRLVVKKARSESELDNIQNRLWDEYELTYTNALVLKKDIGS
                     ISRAQKEISELKENIKTLGIVNVSAIDEFIKTKERFEFMNEQKTDMENAIENLQKVIH
                     EMNILMEKQFSEKFNLINKNFNMVFKELFGGGTANLRLVDKDNILESGIEIDIQPPGK
                     KLQNMMLLSGGEKAFTAIALLFSILLLKPVSFCVLDEIEASLDDANVSRFASYLKKYS
                     QKTQFIVITHRKGSMEVSDALYGVTMQEKGISKVVSMKIGEKAG"
     gene            complement(4853..5578)
                     /gene="rnc"
                     /locus_tag="PK733_13960"
     CDS             complement(4853..5578)
                     /gene="rnc"
                     /locus_tag="PK733_13960"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOJ11683.1"
                     /translation="MEITLNNKYIKELINFENKIIYTFKNKDNLILALTHSSYANENR
                     HSRYNYTSNERLEFLGDAVLNIVISDRIYTKCKNMTEGDMTKARAAIVCEASLVTCAN
                     AIEISKYLLLGKGEELTGGRHRPSILADAFEALIGAIYIDGGIRSARKFINNNINQIF
                     EDAVKGYNYKDHKTHLQEVVQSKGDGRISYDLLEEKGPDHDKTFITRVNIDDETMGVG
                     EGKTKKESEQNAAMLALKKLGVI"
     gene            complement(5663..6916)
                     /gene="fabF"
                     /locus_tag="PK733_13965"
     CDS             complement(5663..6916)
                     /gene="fabF"
                     /locus_tag="PK733_13965"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOJ11684.1"
                     /translation="MNKRVVVTGMGVVSSLGIGISDFWESIKQGKSGISTVTKFDITN
                     YPTKVAAELKNFDPNQFIDKKESKRMDKYTQYAVAAAQIAMEQSALNLEKINKERMGV
                     ILGSGIGGIETLEEQHKILMEKGPGRVSPFFIPMMIANMASGRIAIQYGAKGFNECIV
                     TACATGTNAIGDAFKVIQRGDADIMIAGGSEAPITPLSFSGFCSMKAMSTNSDPSTAS
                     RPFDKDRDGFVMGEGAGVIILEEMEHALARNAAILGEIVGYACTSDAFDHVQPAPEGE
                     GAARCMKLAIEDAVINIEDIEYINAHGTSTYYNDKNETAAIKTVFGEHAKKLAISSTK
                     SMTGHLLGAAGAIEAIICVLALKEGFIPPTINYKTPDPECDLDYVPNIGKSQEINCAL
                     SNSLGFGGHNSTLVIKKIENIHNPA"
     gene            complement(7053..7280)
                     /gene="acpP"
                     /locus_tag="PK733_13970"
     CDS             complement(7053..7280)
                     /gene="acpP"
                     /locus_tag="PK733_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ11685.1"
                     /translation="MVFEKVKKIIVEQLGVEEEDVVMEASFIDDLGADSLDIVELIMA
                     LEEEFEVEIPDSDAEKIAAVGDAVEYIKSHT"
     gene            complement(7362..8105)
                     /gene="fabG"
                     /locus_tag="PK733_13975"
     CDS             complement(7362..8105)
                     /gene="fabG"
                     /locus_tag="PK733_13975"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOJ11686.1"
                     /translation="MQLEGKTAVITGSGRGLGKAVALRLAQMGANIVLNDIANSDAID
                     QTAEEFKAAGYNVIVTRGDVRNYEDVENMINKAVETFGRVDILVNNAGITKDTLMIRM
                     TEKDWDDVLDINLKGAFLCTKATAKVMLKQRNGKIINIASVAGVMGNPGQANYSASKA
                     GLIGLTKSTAKELASRGITCNAVAPGLIKSKMTDVLPEKVKENYLSNIPLGRFGTPED
                     VAAVIGFLASCDSDYVNGQVIHIDGGLVM"
     gene            complement(8138..9061)
                     /gene="fabD"
                     /locus_tag="PK733_13980"
     CDS             complement(8138..9061)
                     /gene="fabD"
                     /locus_tag="PK733_13980"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOJ11687.1"
                     /translation="MSKIAFIFPGQGAQYIGMGKDIADKYKRAGDIFDEASEALGYDI
                     KKMIFESNDDELKITENTQPAILATSIACMQPLLEKGIKADITAGLSIGEYTAHVLAG
                     TFSFRNAVQLVRKRGRYMQEAVPVGVGTMAAIIGLDNDNVINSCKEASSAGIVEPANY
                     NCPGQIVIAGEIKAVEKAVEICKEKGAKKAVILQVSAPFHCSMLKPAGEKLAAELEKV
                     EINNIDIPVVTNVTAKCVTNNDIVKDLLIKQVSSPVLWEDSVKTMIDYGVDTFIEIGP
                     GKALTGFIKRINKDVNIFNVDNIDSLESISI"
     gene            complement(9077..>9756)
                     /locus_tag="PK733_13985"
     CDS             complement(9077..>9756)
                     /locus_tag="PK733_13985"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HOJ11688.1"
                     /translation="AAAFDINAACTGSVYGLTIANQFITTGFYKYVLIVCCEGLSRIV
                     DWKDRSTCVLFGDGAAAMVMGPVEDGYGVLSSHIGADGTLGHNITIPCCYLNEEEINK
                     RPGNNKMVLWMDGSEVFKFAVRVMAQATEKVLEQSGYSIDDIKLIIPHQANIRIIDGA
                     SKRLNISSDRIFTNVHKYGNTSSVSIPLALDESLREGRVKKGDNIILVGFGGGLTWAS
                     ALIKWAK"
BASE COUNT         2692 a         2114 c         1347 g         3603 t
ORIGIN      
        1 attattagtg ctttgcaaag gcgtttttcc attttttgtg tgcagaattc aggttatttt
       61 aaagtgtttt atgtgatttg tcaatataaa tatttggcgc tcgcaatttt attattcatt
      121 ttattcttcg tagaatagcg cctctgcaaa atcatgggga ttaaactcct ggaggtcatc
      181 aatttgctct ccaactccta caagctttac gggaatatcc aattctgatt ttattgctat
      241 cacaattcca cccttggcgg ttccatcaag ttttgtaaga acaattcccg atatgtcaac
      301 agcttcacta aaaacttttg cctgtgaaac agcattttgt cctgtggtag catcaagaac
      361 aagaagaact tcctggcttg catccggcag ctctctatct ataacccgga atatcttttt
      421 taattcctcc ataagatttt tctttgtatg aagacgtcct gcagtatcac agatcaatac
      481 atcagccttt cttgtttttg ctgcctgtat ggcatcatat acaactgctg cgggatctga
      541 tccttcgtta tgcttaatta gttcagatcc tgcacgtttt gaccatattt ctaattgatc
      601 tatggcagct gctctaaaag tatcacctgc tgctagaaca acatttttcc tatttttctt
      661 taaattagcg gcaattttac ctatagtagt tgttttccct accccattaa ctccaactac
      721 aataattatc gagggtgttg tttggatatt taaccttgaa tcaccttcac ttagaatatt
      781 tattatttct tcctttaaaa gtccctttac ttccttcgga tttacaatct ttctttcttt
      841 cacctttttc tttatatctt cggtgatttt taatgtagta tctactccta catcagatgc
      901 tataagtgct tcttcaagtt cttcaaataa ttcctcatca attttaccaa aagaaacaag
      961 tatttggtca attgtacctg taataccttt gcgagttttt tccaggccct gcttaagttt
     1021 atcaaaaagt cccataaata atctcctttg aacacttctt attttaattc ttatgtaaag
     1081 ttttatcagg caagttcaaa ctctagaaaa tcatcctgcc ttttcaccta ttttcatcga
     1141 tactactttt gatatgccct tttcctgcat agttacacca tatagagcat ctgatacctc
     1201 catagaacct ttcctgtgag taattacaat aaactgtgtt ttttgcgaat atttcttaag
     1261 gtatgaagca aacctgctta catttgcatc atccaatgaa gcttctatct catcaagaac
     1321 acaaaatgat acaggcttca gcaatagtat tgaaaaaagc agtgctattg cagtaaatgc
     1381 tttttcacct ccagataaaa gcatcatgtt ttgaagtttt ttacccggtg gttgtatatc
     1441 aatttcaatg ccgctctcaa gtatattgtc tttatccaca agccgcagat tcgctgtacc
     1501 tcctccaaaa agctctttaa aaaccatatt gaaatttttg tttatcaagt tgaatttttc
     1561 actaaactgc ttttccataa gtatattcat ttcatgtatt actttttgca gattttctat
     1621 tgcattttcc atatcggttt tttgttcgtt cataaactca aagcgttctt tcgttttgat
     1681 aaattcatca atggctgaaa cgttaacaat accaagggtc ttaatatttt ctttcaattc
     1741 acttatttct ttttgcgctc tggagatgct cccaatatcc tttttaagta ccagggcatt
     1801 ggtataagtc aattcatatt catcccataa tctgttctga atattatcaa gctctgattc
     1861 agacctggct ttttttacaa caagcctatt caaatcttca ttgagcaaag aaatattcct
     1921 gctaatgtct tccatacttg tcgaagcatt actaacctct tcttcaatga cttttctttc
     1981 ttcagccatt ctatctattt ctatattcct tccaaccttc tcctcttgac aggttttgat
     2041 tgcattacta agaataatat tcttttcatt aaaccattct atttgtcttt cttcttttgc
     2101 tttttcgttt cttttatctg atatgtttct tgaaacagca tctttttctt ctaaaagctt
     2161 ctctatctct ttttttacta tattaatgtc attaattgca gagttaattg ctattttata
     2221 ccctgttaat tctgattgaa ttgaatctct ttccgataga tttcctcgct gggtttcctg
     2281 atactgttct atatcatttt ttagttgaga tatttcctcc tcaatctttt cctgttcgga
     2341 tatatactta tgcagttcaa cccgggtttc ttcctgctga cgtattagtt gttccttttc
     2401 ctgtttaagc atacttgtta tggcagtaat atttttaata ttcatgttgg tattggaaag
     2461 ctgattttct tcccttagtt tagacatttc cctttccttt aagatgcctt gcatcatgga
     2521 aatgtcttta gataactttt ccatattact aataatggtt ttaatatttt cttcaaaatc
     2581 aatcaactct ttattcaact tgtgaatatc ctcttcaagt tcagatattt gacctgttct
     2641 gcccagtatt cctgttgcat ttacatccct gctgcctccg gacattgctc ctgaagtgtt
     2701 aagtatgtca ccttccagag ttactatcct gaaactataa ttgaatttcc gagccatata
     2761 tattcctgaa tcaaggtctc tgacaacaat aaccctcccg agaaagctta aaactatact
     2821 gttatattca gagttatatt ctaccagatc agaagctacg ccgcaaaatc cggaacattt
     2881 tttaacttct tttaaaatgt tgctatccag ataattgcct tttacagatg taataggcaa
     2941 aaatgttgcc cggcccatac tgttcttttt taaaaattca atagctcttt ttgcatcctc
     3001 ctcggtttca gcaacaatgt tttggattgc gcctcccaga gccatttcaa tggctgttga
     3061 atatcccttt tttacggata tcagttgtcc cagcgcccca caaattcctt ttccggtaga
     3121 atcggctttc ttacaagctt gcaagaaggc ttttactgtt ctattataac cttccagatt
     3181 atcttccatt cctttaagca ttttttgctt ggataccttt acttgtatat ctgaagatac
     3241 agaatttttc ttctctttct caattgacaa cctggagtct aattcttttc ttgaggatat
     3301 aagagcctca agagatgaca tccccgtatt aagctcctga attttcaatt caatactttt
     3361 ttctaatgtt tctttctttt gaatctcttt agctatattt aaatccaatt ctctaacttc
     3421 gttttcaatg tgctgctgct gtttacataa actctctata tgactattaa cattgcctac
     3481 ttgaattttt ttatcggaga gcaggtcaag cttgttcatt atggttgaat tcattgtttc
     3541 aagatgcttt tcatgagtat taagagtcaa aagtatttct tctcttttct gctcacattt
     3601 tttaatatca tccagtaaat ggtcatgcac agtaataaaa tcctcaagct ttttatcatt
     3661 gtcagctaat atactggata ttttattaat tctattgctt aagttagaaa tttcattgtc
     3721 aattctataa gtattttgct ttagattact tattttttcg ttgttaagat tcaattcccc
     3781 ctgatttttt tcaaggcttg tttcaagagc ataatattcc tttctgcaac tatcaagctt
     3841 gtcctcaaga tttttaaggt atttactttt attctcatta tcatttgaag tcaaattata
     3901 cttgtgtaca agaccatttt tgctttccat aacggaatta taatgcccat cacattctgc
     3961 tattttctct ttcaatttag atataaggtc aaggtaaaca tttatatcaa gttcctttaa
     4021 cctttctctc aatgacaaat attttctggc tgttgatgcc tgctcttgta gaggttccaa
     4081 acgactctcc agttcatgca aaatatcatc tatcctaagt agattttgct ttgtaagatt
     4141 aagttttttt tctgcttcct cttttctgac cttatatttc attataccgg aagcttcttc
     4201 aaatatattt ctcctatctt ctgattttgt acttagaatt tcatctattc taccttgtcc
     4261 aataatagaa tagccgtctt ttccaacacc tgtatcaagg aaaagattat taatgtcctt
     4321 aagccggcag ggtgttttgt ttatataata ttcactttca cctgatctga aaaatcttct
     4381 tgaaatagtt acttcagaat attcaagagg gagatcacct tcagagttat caatggttaa
     4441 tgctacttca gcgaatccta caggttttct gaattcagta cctgtaaaga ttacatcctc
     4501 aagtttgctt ccccttattg tttttatgct ttgttcacca agtacccacc gtatggcatc
     4561 cactatgttg ctttttccac acccatttgg ccctacaata gatgtaatgc ccggatcaaa
     4621 atccagggtg gttctatcgg aaaaagattt aaacccctgt atatccaacc tcttaaggtg
     4681 caaaatataa ccctcctatc aaaatatata taacgcaaaa agattttacg tggtccacta
     4741 accctaaaaa taatctatta tggaccacaa aatttttata gtgtagtttc ttttgtgcgt
     4801 tatctataaa aaaagaaagt gcaaattaag atataacttt tcgagatcta tactatatga
     4861 cccctaattt ctttaatgct aacatggcag cattttgttc agattccttc ttggtcttac
     4921 cctcaccaac acccatagtt tcgtcatcta tatttacccg agtaataaaa gttttatcat
     4981 ggtcaggtcc tttttcctct aataagtcat aacttattct gccgtcacct ttgctctgaa
     5041 caacttcctg taaatgggtt ttgtgatctt tataattata tcctttaaca gcatcttcaa
     5101 atatttgatt tatattgtta ttaataaact ttcgtgcact tctgattccg ccatctatat
     5161 atattgctcc aatcagtgct tcaaaagcat ctgccaatat ggacggtcta tgcctcccgc
     5221 cggttaattc ctcacctttt cccagtaata aatatttgga tatttcaatg gcattagcac
     5281 aagttaccaa agatgcttcg catacaattg cagcacgtgc tttcgtcata tcaccttcag
     5341 tcatgttttt gcactttgta taaatcctat cactaataac tatatttaaa actgcatccc
     5401 caaggaattc cagtctttcg ttactagtgt aattatacct gctatgcctg ttttcattgg
     5461 cataggaact atgcgtcaag gcaagaatta gattatcttt atttttaaat gtataaatta
     5521 ttttattttc aaagtttatt aattccttaa tatatttgtt atttagggtt atttccattt
     5581 catcacctat ctttaaccta tctaaattaa aaaagtatag taataccata tatgaagtat
     5641 taagcattgc atagccaaat attcatgctg gattgtgtat attctcaatt ttttttatta
     5701 ccagtgtgga gttatgtcct ccaaacccca gtgaattgct cagagcacag tttatttcct
     5761 gagatttgcc tatattaggt acatagtcaa ggtcacattc aggatccgga gttttgtaat
     5821 ttattgtagg agggataaag ccctctttaa gtgcaagaac acatatgatt gcttctattg
     5881 cacctgctgc acccaggaga tgccctgtca tggatttagt agaacttatg gcaagttttt
     5941 ttgcatgttc tccaaaaact gtttttattg cagcagtttc atttttatca ttataatatg
     6001 tcgaagtacc gtgggcatta atgtattcta tatcttctat atttatcacg gcatcctcaa
     6061 tagcaagttt catacacctt gctgcacctt ctccttccgg tgcaggttgt acatgatcaa
     6121 aagcatcact ggtacatgca taaccaacta tttcgcctaa tatagctgca ttcctagcaa
     6181 gagcatgttc catttcttca agtataataa cacctgcccc ttcacccatt acaaatccat
     6241 ctctatcttt atcaaaaggt cttgatgctg ttgaaggatc actattggta gacatagcct
     6301 tcatagaaca aaagccagaa aaagacaacg gagttatagg tgcttcagaa cctcctgcta
     6361 tcataatatc ggcatcacca cgctgtatca ctttaaaagc atctccaatt gcattagttc
     6421 cggtagcaca agcagtgaca atgcattcgt taaatccttt ggctccgtac tgtattgcaa
     6481 tacgtcccga tgccatattt gcaatcatca ttggtataaa aaacgggctt actcttccag
     6541 gacctttttc cataagaatc ttatgctgtt cttcgagagt ttcaatgcct cctatgccgg
     6601 aaccaagaat aacccccatt cgttctttat ttatcttttc cagattcaat gcagactgtt
     6661 ccatagctat ttgcgcagca gctactgcat attgtgtata tttatccatt cttttagact
     6721 cttttttatc gataaactgg ttagggtcaa agtttttaag ttcagctgca actttagttg
     6781 gatagtttgt tatatcaaat ttagttactg tactaattcc tgatttccct tgcttaatag
     6841 actcccaaaa atcactaatt cctattccta atgaagacac aactcccata cccgttacaa
     6901 ccacacgttt attcatcaga taaacctcct attcccttat gcaatatata ttacataaat
     6961 ctactaataa atttaatata atttttaaat aatgtcaatt aaaagagtta aggtgggtac
     7021 atttaaaaaa agtcccggca tcgggacttt ttttatgtgt gacttttaat gtactctaca
     7081 gcatcaccaa ctgctgcaat tttttcagca tcactatcag gtatttcaac ttcgaactct
     7141 tcttccagcg ccataatcaa ctcaacaata tcaagtgaat ctgctcccaa atcgtctatg
     7201 aaggaagctt ccataactac atcttcctct tcaactccca gttgttctac gataattttc
     7261 ttaacttttt caaaaaccat atgctcacct ccttccacag gaaaaaacaa aaactttcag
     7321 ttgttgctaa tagcaactat aattctatag taagaatatt attacattac taatccgccg
     7381 tcaatatgta ttacctgtcc atttacatag tctgaatcac aagatgcaag aaatccgata
     7441 acagccgcta catcctcggg tgtcccaaac cgtccaagag ggatattact cagataattt
     7501 tcttttactt tttcaggcag cacatcggtc attttacttt ttattaaacc aggagcaaca
     7561 gcattgcatg ttattcccct ggaagccagt tccttcgcag tagattttgt aagcccgatt
     7621 aaacctgctt tagatgcaga ataatttgcc tgaccgggat ttcccataac acctgcaaca
     7681 gaagctatat ttattatttt accgtttctc tgcttaagca ttactttagc ggttgccttt
     7741 gtacataaaa aagcaccttt caagttgata tcaaggacat catcccaatc tttttcagtc
     7801 attcttatca ttaaggtatc tttagttatg ccggcattat ttaccagtat atctactcgc
     7861 ccaaaagtct caactgcttt gttaatcata ttttcaacat cttcataatt tcttacatcg
     7921 cctcttgtta ctataacatt ataaccggca gctttgaatt cttctgctgt ttgatctatg
     7981 gcatctgagt ttgcaatatc atttaacaca atattagctc ccatctgagc cagtctaaga
     8041 gcaacggctt tgccaagtcc ccttcctgaa cctgtaatta cagcagtttt accttctaac
     8101 tgcatattaa cattcctccc tcatttgcta tcttcattta tatactaata ctttcaagtg
     8161 aatctatatt gtcaacatta aatatattaa catccttatt aattcttttt ataaaacctg
     8221 ttaatgcttt accaggacct atttcaataa atgtatcaac accataatca atcattgttt
     8281 taacagaatc ctcccacaaa acaggactgc ttacctgctt tataagaagg tcttttacaa
     8341 tgtcattatt agttacacat ttggctgtaa cattagtcac tactggtatg tcgatattat
     8401 ttatttccac cttttcaagc tctgctgcca gcttttcacc tgcaggcttt aacatgctgc
     8461 agtgaaaagg tgcgcttacc tggagtatta ccgctttctt tgcacctttc tccttgcaga
     8521 tttccactgc tttttcaact gctttaattt ccccggcaat aactatctga ccggggcaat
     8581 tataatttgc aggttccaca atcccggctg aggatgcctc tttgcaggaa ttgattacat
     8641 tatcattatc caaccctata atagccgcca ttgtgccaac tcctaccggt acagcttcct
     8701 gcatatatct acctcttttt cgtaccaact gtactgcatt cctaaatgaa aatgttcctg
     8761 ccaaaacatg agctgtatat tctccaatac tgagtcctgc agttatgtca gctttaatac
     8821 ctttttccag taaaggctgc atgcaggcaa tgctggttgc caatattgcc ggctgagtat
     8881 tttcagtaat ctttagctcg tcgtcattgc tttcaaaaat catcttttta atgtcataac
     8941 ctagcgcttc agacgcttca tcaaatatgt ctcctgctct cttatatttg tctgctatgt
     9001 ctttacccat tccaatatat tgtgcaccct gaccgggaaa aataaaagct attttgctca
     9061 taattaaccg ccccctctat tttgcccatt ttatcaaggc tgatgcccat gtcagccctc
     9121 caccaaaccc caccagtatt atattatcac ctttttttac ccgaccttcc cttaaacttt
     9181 catccaatgc caacgggatg gaaacagatg aggtattgcc atatttatga acattggtga
     9241 atattctgtc gctggaaata tttaaccttt ttgaagcacc atcaattatt ctgatatttg
     9301 cctgatgggg aataatcaat tttatatcat ctatggaata accagactgc tccagaactt
     9361 tttccgtagc ttgagccatt actcttacag cgaatttaaa gacttcactc ccatccatcc
     9421 ataacaccat cttattattg cccggtcttt tatttatctc ttcttcgttt aaataacagc
     9481 atggaattgt aatattatgt cccaaggtgc catccgcacc tatatgagag gagagtactc
     9541 catagccgtc ctctaccggt cccattacca ttgcagcagc tccatctcca aagagaacac
     9601 atgtacttct atccttccag tctactattc tggatagtcc ttcacaacat actattaaga
     9661 catatttata gaatcctgta gttataaact gattagctat agttaaacca tatacggaac
     9721 ctgtacatgc agcgtttata tcaaatgctg cagcat
//
LOCUS       DAOSDD010000176         9722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2623_length_9722_cov_5.094548, whole genome shotgun sequence.
ACCESSION   DAOSDD010000176 DAOSDD010000000
VERSION     DAOSDD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_9722_cov_5.094548"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1736
                     /locus_tag="PK733_13990"
     CDS             264..1736
                     /locus_tag="PK733_13990"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ11689.1"
                     /translation="MRVPYGISNFEKIRQENYLYIDKTKYIELLEDDVPYQFFIRPRR
                     FGKSLFLSTLENYYDLNRGQLFDELFNGLYIGENPTKRRNTYLILFLSFSKVITSEGK
                     ERLIESFDNLVISEVSNFLRYYDPIFNEKGIPENITGAEMAINYVVNRAKQEGKKILI
                     LIDEYDNFANDLITTGNKELYYGLLSSEGYVRSFYKTIKDGTMSSIDRVFMTGVSPIM
                     LDDMTSGFNITKNLTLDGKYNEMLGFTREEVMNIVDKLHSVKPLDKKVLMEDMVNYYN
                     GYLFCRNGRERVFNSNMVLYFINALIQDGNYPENMLDMNMKTDYKKIESMAFNFQDED
                     TIHTLITEDTITTQLVEKFNLEYMYSNKENFISLLFYMGMLTIKDVIFKEYTLTVPNY
                     VIKSIFWEYFIEKISYRDKVDIKIQELSTAIRSMAVEGKIDLLSTYMNDLMTQLSNRD
                     LIAFDEKHLKMILNTVFSLSGIYVVNSEYEVENGYRYIPC"
     gene            1711..1962
                     /locus_tag="PK733_13995"
     CDS             1711..1962
                     /locus_tag="PK733_13995"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HOJ11690.1"
                     /translation="MDIDIFLAKNKAFEQSIRYEWIIELKYLKEQDRNKFNKVLQDAE
                     NQLKCYANSSRIKEMKNIGKIKRAAVIMVGKDNIFMKLI"
     gene            complement(2272..2958)
                     /locus_tag="PK733_14000"
     CDS             complement(2272..2958)
                     /locus_tag="PK733_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ11691.1"
                     /translation="MSAVNICIFGDSIGKGVVLQPNTSRYEVVKMNSEKLFGQDEINI
                     KNYSMFGCTISKGLSIIKRHVRELMGYNNIFLELGGNDCDFAWNEIADNPEKQHVPKT
                     PLGEFRELYKQAIDEIHSNGGNPIILTLPPLEPNRYFNWISRGINSDNILKWLGSVDM
                     IYRWQEMYNVEVMLLASKMAVPIIDIRSAFLKCSNYFDFLCSDGIHPNKAGYELIYKT
                     IFEQYKNKKK"
     gene            complement(3009..3791)
                     /locus_tag="PK733_14005"
     CDS             complement(3009..3791)
                     /locus_tag="PK733_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11692.1"
                     /translation="MEKKLRKYLNRKFLLYPKTNKILEVREELYSIMLDKYNDCLSSG
                     MSEDESYKNAVEMMIDYKDAIREVETGSSLSALKKKLINTAFVTSFYYITLTFIYLFV
                     SMVVLKTFEKTWLIVVGGAFTYLVYFSVTIYGYAKLFNFKTLARCGIAIIYFNLVPVF
                     YVFPSLYLSTVYSKNVWNRSWLVAVVILFFYILTDYIVYLKQSTVLEKSIHLLALGFT
                     LTTIIYLSMSMWFQLWGIAWIIYVVYLAIVTLVLYIIEKRKG"
     gene            complement(3791..4123)
                     /locus_tag="PK733_14010"
     CDS             complement(3791..4123)
                     /locus_tag="PK733_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ11693.1"
                     /translation="MSYSSEILRGNTETIILAILIKKDSYGYEIMKTIIENSKGLFNI
                     KDATVYTAIKRMEKDELITSYWGGEDDGARRKYYRITEKGKDVYAQKVDEWKEINIVL
                     NNLIGGGL"
     gene            4492..5397
                     /locus_tag="PK733_14015"
     CDS             4492..5397
                     /locus_tag="PK733_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ11694.1"
                     /translation="MKSCLFLCSFGSLFSKGLSLNEAVDMAVEYGFDAVEPYPVNELS
                     TYNLTAAKEFGEYCHSKEVAIPCFSMLAKLEGTNYKEEVERLKGFIDMAAAMGAGLFH
                     HTLVPSLQHNAAQMPLLKNILPQLISACEELCDYASRHNITCCYEDQGFVINGIRAYE
                     EFYLSLGRANKGVVADLGNILFVSEKPQDFVGHFAPLVKHVHIKDYLVKPGSSQFPGK
                     GWYLSANGDYLRGTIVGHGTVDFTQTVKILLRADYNGYYSFEFDGMEEPGEALAYGLA
                     NLRYYFEEAKRQLNAFEGKKLSSNI"
     gene            5548..5958
                     /locus_tag="PK733_14020"
     CDS             5548..5958
                     /locus_tag="PK733_14020"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ11695.1"
                     /translation="MGINDIIAKYNLRLLLIFGSYNTDRFTENSDIDLAYLSDRNLNI
                     EEEMELLMDMVVHFMRDGIDLVNLNKADPLLSFQIACNSKVLYEENNSYILFKMKASA
                     RYADTKHLRVLRRKFLQEQLKSSGYIEKTEMEVI"
     gene            5958..6380
                     /locus_tag="PK733_14025"
     CDS             5958..6380
                     /locus_tag="PK733_14025"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOJ11696.1"
                     /translation="MGNFEVIIEKLIKLREYIEQIKKIKPDSYEQYVSKITIKYAIER
                     IIQLIMDIALDINNILLAYNKRPPAPDYYNSFIDIAECGVLDSAFAVSIAPSTGLRNR
                     LIHEYEKINDEIVFNSINKIISEYQKYVVMVFEYIEKT"
     gene            complement(6516..7460)
                     /gene="pdxS"
                     /locus_tag="PK733_14030"
     CDS             complement(6516..7460)
                     /gene="pdxS"
                     /locus_tag="PK733_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOJ11697.1"
                     /translation="MDLCKNKTTNANYKDNTNAGKNVITRYNSGNNLAEKLKGGVIMD
                     VTTPEEAQIAEEAGAVAVMALERVPADIRKQGGVARMSDPGMIKRIMNTVSIPVMAKA
                     RIGHMVEAQILEALRIDYIDESEVLTPADDVYHINKWDFKTPFVCGARNLAEALRRIG
                     EGACMIRTKGEAGTGNIVEAVRHMRLIMGEISKIVAMKEDELMVTAKELGAPYDLIQY
                     VHNKGRLPVVNFAAGGVATPADAALMMQLGSEGVFVGSGIFKSANPAKTAKAIVTAVA
                     NYNNPEVLLEVSQNLGEAMKGLEISEIQPEYLLSKRGV"
     gene            7612..9102
                     /locus_tag="PK733_14035"
     CDS             7612..9102
                     /locus_tag="PK733_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ11698.1"
                     /translation="MKCVSIQIDREGSEPLYIQIYNEIRKLIMEGCYNNNERLPSIRS
                     LSKQLGVNSVTIINAYRLLQNDGFAVVKTGSGAYVRKRELKVISAGSESISDELYTSE
                     DIELMNKGSIDISSAIVNFATTTPSPDIFPLEDFKRAIIETMDSDGAEAFNYQESLGF
                     EGLRQIISKEFLSKGINEQTHNIQIISGAQQGIDIVAKALLGRDDCVVVERPTYVGAN
                     AVFKSRGAKIVGVDIKNDGMDMEELASVMRKYRPRIIYTIPTYHNPTGYVYSLEKRNE
                     LLRLAAAFGSYIIEDDYLSELNYYGTTAVSLKKLDENDRTIFVKSFSKLLMPGLRIGF
                     MAVPDHLMEEINSAKRATDISTSGLIQRAFSRYIEAGLWEKHLSRIKQIYIKRYELML
                     SYLNDMKEYGLKFTQPGGGFNFWVKLPIGMDANMLYYQTSKRGVVFIPGSIFSYGRST
                     IGADYVRLSFAQANEQDIKYGMSVLKDCIENITKGYSSTNRVYPLV"
     gene            9383..>9722
                     /locus_tag="PK733_14040"
     CDS             9383..>9722
                     /locus_tag="PK733_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ11699.1"
                     /translation="MKVLFIGGTGTISTAVSELAVKKGIDLFLLNRGNRGEFFPEGAT
                     LIRGDIRNIETIHKILDDYKFDVVVDWVAFTPDHIEADIGLFKGKIGQYIFISSASAY
                     QRPPAHYLVDE"
BASE COUNT         3249 a         1543 c         1802 g         3128 t
ORIGIN      
        1 ttttggctat aaacggggaa atgccaaatg ccatattttg gactaaatgt aatttacaac
       61 ccaagggtaa aaaatagtat atgatttttt actcctcaga gctggcactc aaagtttgtt
      121 attgatttgt ccgcattgat tcgatattat atagtatata cactaagtcc attcctatta
      181 tttgaaaaca agggggtagc ttgaaaaata aattttcaat tgctgcctga gccaccgaca
      241 gtaactgcag aatggaggta aatatgcgag ttccatatgg tatatccaat tttgaaaaaa
      301 ttaggcaaga aaactatctt tatattgata aaacaaaata catagaactt ttggaggatg
      361 atgttccata ccagtttttc attaggccca ggcgttttgg caagagcttg tttttatcta
      421 cccttgaaaa ttattatgat ttaaacaggg gccaactatt tgatgaacta ttcaatgggc
      481 tatatattgg agagaatcca acaaagcgca gaaatacata tttaatttta tttttaagct
      541 tttcaaaagt aataaccagt gaaggaaaag agagacttat tgaaagcttt gataatcttg
      601 ttatttctga ggtaagtaat tttcttagat attatgaccc gatctttaat gagaaaggca
      661 tcccggaaaa tataacagga gcagaaatgg ctattaatta tgtagtaaac agggctaagc
      721 aggaaggtaa aaaaatactt atccttattg atgagtatga taattttgca aatgatttaa
      781 taacgacagg caataaagaa ttgtattatg gtttactttc tagtgaaggt tatgtgagaa
      841 gcttttataa aacgataaaa gatgggacta tgagcagcat tgatcgtgtg tttatgaccg
      901 gagtcagtcc gattatgctg gatgatatga ccagtggttt taatatcaca aaaaacctga
      961 cacttgacgg caaatataat gaaatgctgg gatttaccag ggaagaagta atgaatattg
     1021 ttgataagct tcactctgtt aagcctctgg ataagaaagt ccttatggag gatatggtaa
     1081 actattacaa tggatacctt ttttgcagga atggcaggga aagagtattt aattccaaca
     1141 tggtgcttta ttttataaat gctttaattc aagacggaaa ttacccagag aatatgcttg
     1201 atatgaatat gaaaacagac tataagaaaa tagaatcaat ggcatttaat tttcaggatg
     1261 aagatactat tcatacttta ataacagagg atactataac cacccagttg gtagaaaagt
     1321 tcaatctcga atatatgtat agcaataaag aaaattttat atctcttcta ttctatatgg
     1381 gaatgcttac tataaaagat gttattttta aagaatatac attgactgtc cctaattatg
     1441 ttataaaaag tattttctgg gagtatttca ttgaaaagat ttcatatcgg gataaagtgg
     1501 atattaaaat acaagagcta tctactgcta taagaagcat ggcagtagaa ggtaagatag
     1561 atttactatc tacctatatg aatgatttaa tgacgcagct ttccaatagg gatcttattg
     1621 cttttgacga aaaacatttg aagatgattt taaatacggt attcagttta agcggtattt
     1681 atgttgtaaa tagtgaatat gaagttgaga atggatatag atatattcct tgctaaaaat
     1741 aaagcatttg aacaatctat tcgatatgag tggataatag agttgaaata cctgaaagaa
     1801 caggatagaa ataaattcaa taaagttttg caggacgcag aaaatcagtt aaaatgctat
     1861 gccaatagca gcagaattaa agaaatgaaa aatattggca aaattaagag agcggcagtg
     1921 ataatggttg gaaaggataa tatttttatg aaattaatat aaccctagga gtatgctttg
     1981 atagctcaga ctttccgagc agactcctag taagcagcgg caaagcttat atttctaaag
     2041 ccttgaaatc tgtgtgttgc tggaaagaga actgggtctt gtaagctcat atgtagagat
     2101 agaaaaggcg cggtttgggg acaatcttca ggtaatatcc gatattagca atacgataaa
     2161 gaccgggata cctttgcttt ctatacagcc attggtagaa aacgccatta ttcatggtct
     2221 gagaaagaaa ggcggtgtgg gtactgtttt ctctttgcaa tatcctctat actacttttt
     2281 tttgttttta tattgttcga aaattgtttt ataaataagt tcatatccgg ccttattggg
     2341 atgaatacca tcagagcata agaaatcaaa ataattactg catttcaaaa acgcactgcg
     2401 tatatctatt attggaacag ccatctttga tgccaatagc ataacctcaa cattatacat
     2461 ctcctgccag cggtaaatca tatcaacact tccaagccat ttcagtatat tgtcactatt
     2521 aatgcctctg gaaatccagt taaaatatct gtttggttct aatggaggca gagtaagaat
     2581 tattgggtta ccgccgttgc tatggatttc atcgattgct tgcttatata attccctgaa
     2641 ttccccaagt ggcgttttgg ggacatgttg cttttcagga ttatctgcaa tttcattcca
     2701 cgcgaaatca cagtcattac cgcctaattc caaaaaaatg ttgttatacc ccataagctc
     2761 acgaacatgc cgtttaataa ttgacagtcc ttttgagatt gtgcaaccaa acatggaata
     2821 attttttatg tttatttcat cctgcccaaa tagtttttca gaattcattt ttactacttc
     2881 ataacggctt gtattaggct gtaatacaac tccttttcca attgaatctc caaatataca
     2941 gatatttaca gcactcatat ttattccacc tcttatacta aattattttg cctcttatgt
     3001 ttggcttttt aaccttttct tttttcgata atataaagaa ctaatgtaac aattgctaaa
     3061 tatactacat atataatcca tgctatgccc caaagttgaa accacattga cattgacaaa
     3121 taaataatag ttgtcaatgt gaatcccaat gccagaaggt gaatggattt ttcaagtacc
     3181 gtagattgtt ttagatatac tatgtaatca gttaaaatgt aaaaaaacag aataactacc
     3241 gcaaccaacc aactacgatt ccatacattc ttagaataca ctgtagataa ataaagggat
     3301 gggaaaacat aaaatacagg tactaaattg aagtaaataa tcgctatgcc gcatctagct
     3361 agagttttaa aattgaataa ttttgcatat ccatatattg ttactgaaaa ataaactaaa
     3421 tacgtaaaag cgccaccaac aactataagc catgttttct caaatgtttt aagaacaacc
     3481 atacttacaa aaagatatat aaatgttagc gtgatgtaat aaaaggaagt tacaaatgca
     3541 gtatttataa gtttcttttt cagtgcactt aaggaactgc ctgtttcaac ctctctgata
     3601 gcatctttat aatcaatcat catttcaact gcattcttgt aactttcatc ttcagacatt
     3661 cctgaactaa gacaatcatt atacttatca agcatgattg aatacaattc ctcgcgaact
     3721 tccaaaatct tattagtttt aggatataag agaaactttc tatttaaata tttccttaat
     3781 tttttctcca tcacaagccg cctccgatca agttattcaa tacaatattt atctctttcc
     3841 attcatcgac tttctgagca tacacatcct tgcctttttc ggtgattcta taatattttc
     3901 gtcttgcgcc atcatcttcg ccaccccaat agcttgtaat caattcatct ttttccatgc
     3961 gcttgatagc agtataaaca gtggcatcct ttatgttgaa caaaccttta ctgttttcaa
     4021 taatggtttt cataatttca taaccatatg aatctttttt tataagaata gctaaaataa
     4081 ttgtttcagt atttcctctt aatatttcac tagaataact caacacatct cctccactac
     4141 ctaatcagat tagatatatt atatggttat tgcttttatt tgtcaactat tattaattca
     4201 catattatat ggatagatag cgggaaagac attagtctgg aacgctatgc gcatctgcct
     4261 gttttagata tgataaaatc gcataaacat attaatgctt ttagtaaaca ttattgcgat
     4321 acaccacaat atgatttaga aaataacgat attggaatat aagggaagtt acttttttaa
     4381 ttgagggtgt caaaaaaagt ggtcattatg gttaaaaaaa gagttgaaaa aagtagctaa
     4441 taatggtata gttattaaga tttgctaaca ccaattattg ggggtatagt tatgaaatcc
     4501 tgtttatttt tatgcagttt tggctctctt ttttcaaagg gattaagcct gaatgaggct
     4561 gtcgatatgg cagttgaata tggatttgat gctgttgaac cttatcctgt caatgaactt
     4621 tcaacatata acctgactgc tgctaaagaa tttggtgagt actgccacag caaggaagtt
     4681 gccatcccgt gtttttcaat gcttgccaag cttgaaggta caaattacaa agaagaggta
     4741 gaacgcctga agggatttat agatatggct gctgccatgg gtgccggttt gttccatcat
     4801 actcttgttc cttctttaca gcataacgca gcacaaatgc ctcttttaaa gaatatttta
     4861 ccgcagctga tttctgcatg tgaggaactc tgtgattatg cttcccggca caatatcact
     4921 tgttgttatg aagaccaggg ctttgtcata aatggtattc gtgcatatga ggagttttac
     4981 ttgagcttgg gaagagctaa caaaggtgtt gttgccgatc ttggcaatat actttttgta
     5041 agtgagaaac ctcaggattt tgtgggccat ttcgcacctt tggtgaagca tgtacatatt
     5101 aaggattatc tagttaagcc cggcagcagt caatttccgg gaaaaggatg gtatttaagt
     5161 gcaaatggag attatttgcg tggtactata gtaggtcatg gcactgtaga ttttactcaa
     5221 acagtcaaaa tactattgcg ggctgattat aacggctatt actccttcga atttgatggt
     5281 atggaggagc ctggtgaggc acttgcatat ggtttggcga atcttcggta ttattttgag
     5341 gaagcaaagc gccagttaaa tgcattcgag ggtaagaaac ttagtagtaa tatttgaaga
     5401 atgtgtcttg aaagaatcgt ccatatttga cttgctgaaa aaatgactaa cttatgcaaa
     5461 aataggattg aagtacagat aatttgatat aatataaaat agagatgatt aagttgagat
     5521 gataatcaaa ctgatgggga gttgtatatg gggataaacg atattatagc taaatataac
     5581 ttgcgcttat tattaatatt tggatcatat aatactgaca ggtttacaga aaatagtgat
     5641 atcgatttgg catatttatc agatagaaat ttaaacatag aagaagagat ggaacttctt
     5701 atggatatgg ttgtccattt tatgcgcgat ggtatagact tggtaaattt gaataaggct
     5761 gatccacttt tatcatttca aatagcatgc aactccaaag tgctatatga agaaaataat
     5821 agttatattc tgtttaaaat gaaagcatca gcaagatatg cagacactaa gcatcttagg
     5881 gttttaagaa gaaaatttct acaagaacag ttgaaatcgt cagggtacat agagaagact
     5941 gaaatggaag tgatttaatg ggtaattttg aagtgattat tgagaaatta ataaaattaa
     6001 gagaatatat tgaacaaatt aaaaaaatca aaccagatag ttatgaacag tatgtttcga
     6061 aaataactat aaaatatgca atagaaagaa taatacagtt aattatggat attgcactgg
     6121 atataaacaa tattttactt gcctataata aaagaccacc tgcacctgat tattacaatt
     6181 catttatcga tatagcggaa tgtggtgtac ttgatagtgc ttttgctgtt tctatagctc
     6241 cttcaactgg tttacgaaac agacttatac atgaatatga gaaaataaat gatgaaattg
     6301 tgtttaatag tattaataaa attattagcg aatatcaaaa gtatgtagtt atggtttttg
     6361 agtacataga aaagacataa aaaataaaca tgtatttatt aaacggtgtg aggcaatccg
     6421 attagtggat tgcactgatg taataacaaa gatagaaggt tcttgatctt ccaacacagg
     6481 caagacaaga accatttcca tttatctgta aatctctaaa ctccacgttt ggaaagcaga
     6541 tattcaggtt gtatttcgct tatctccaga cctttcatgg cttcacccag attctgggaa
     6601 acttcaagaa gtacttccgg gttgttatag ttagctactg ctgttacaat agcttttgct
     6661 gtttttgccg ggttcgcaga tttgaatata ccggacccta cgaaaacgcc ttcactgcca
     6721 agctgcatca tgagagcagc atctgcaggt gtagctactc ctcctgctgc aaagttaact
     6781 accggcaggc ggcctttatt gtgaacgtat tggataaggt cgtaaggagc accaagttcc
     6841 tttgctgtca ccataagttc atcttctttc attgcgacaa ttttacttat ttcacccata
     6901 ataagacgca tatggcgaac tgcctcaaca atattgccgg tacctgcttc tccttttgta
     6961 cgtatcatgc aggcaccttc accgatcctt cttaaagctt ctgcaagatt tctggcgccg
     7021 catacaaaag gggttttgaa atcccatttg ttgatatgat aaacatcatc agcaggggtc
     7081 agtacttcac tttcgtctat gtagtcaatt ctcagtgctt caagtatctg tgcctcaacc
     7141 atgtgtccga tccttgcctt tgccataacc ggtatggaaa cggtattcat gattctttta
     7201 atcattccag gatctgacat ccttgctaca ccgccctgtt ttcgaatatc tgcaggaact
     7261 ctttcaagag ccataacggc aacagcaccg gcttcttctg caatttgtgc ttcctcaggt
     7321 gtggtgacat ccattattac tccacccttt aacttttcag caaggttatt tcctgagtta
     7381 taccttgtaa ttacattttt accagcatta gtattatctt tataatttgc atttgtagtt
     7441 ttattcttac ataaatccat atcaaaaact cctttacaaa taatttcttt tagtataaaa
     7501 tagcattaaa aagcgtatcg atacaattaa ttaaaataca cctttaatta ttttgatatt
     7561 agctatttta acaagattac aatattattc aatgaaagtt ggtgaaataa cttgaaatgt
     7621 gtatcgatac aaatagacag agaaggtagt gaacctctct acattcagat atataacgaa
     7681 attagaaaat tgattatgga aggctgctat aataataatg aaaggctgcc atcaattaga
     7741 tctctatcga aacaattggg agtaaactca gttacaatta ttaatgcata tcgtctgctg
     7801 caaaatgatg gatttgcggt ggtgaaaaca gggagcggtg cttatgtgag aaagcgggag
     7861 ttaaaagtaa tatcagccgg ttctgaatct atatcggatg agctttatac aagtgaagat
     7921 atagaactaa tgaataaggg tagtattgat attagttctg ctatagttaa tttcgctact
     7981 acaaccccat cacctgatat ttttcccttg gaagatttta aaagggcaat tattgaaact
     8041 atggattcgg atggagcgga ggcgtttaat tatcaggaaa gccttggatt tgaggggctt
     8101 cgccaaataa taagcaaaga gtttctttca aagggaataa acgaacagac acacaatatc
     8161 cagattattt caggtgcaca gcagggtata gacattgtag ctaaagctct tctaggtcgg
     8221 gatgattgtg tagttgtaga gagacctacc tatgtgggcg caaatgcagt ttttaaatcc
     8281 agaggagcta aaattgttgg tgttgatatt aaaaatgacg gaatggatat ggaggaactg
     8341 gcttctgtaa tgagaaagta caggcccagg attatatata caataccaac ctaccataac
     8401 cccacaggtt atgtctattc tcttgaaaaa agaaatgaac ttctgcgtct ggcagcagcg
     8461 tttggaagct atattataga ggatgattac cttagtgaat tgaattatta tggaactact
     8521 gcggtatcat taaaaaaact ggatgaaaat gaccggacaa tatttgtaaa aagcttttca
     8581 aagcttctta tgcctgggct tcgtatcggt tttatggcag tacccgatca tcttatggaa
     8641 gaaatcaata gtgctaaacg agcaacggat atttctacct caggtcttat acagagagcc
     8701 ttttcccgtt atattgaagc aggtctgtgg gaaaaacatt tgagcagaat aaagcagata
     8761 tacataaaaa gatatgagct tatgctatct tatttaaatg acatgaaaga atatggcttg
     8821 aaatttacac agcctggagg tggttttaac ttctgggtta aacttcctat aggaatggat
     8881 gccaatatgc tttattatca gacatctaaa aggggtgtag tatttatacc cggaagcatt
     8941 ttttcatatg gacgaagcac cataggggcg gattatgtcc gtttaagctt tgcccaggcg
     9001 aatgaacaag atatcaagta tgggatgtca gtactaaaag attgcattga aaatatcacc
     9061 aaaggctata gcagtacaaa tagggtttat ccacttgtat aggcttatag tgtctttcct
     9121 ttataagttt taagagtgca tttgatcaat gcacacaaat agtacaatag tactaatggt
     9181 attgaccaat gtatacctaa gtagtgtata atatgcttat aaataaagag caaatactgc
     9241 atggttagaa aatatatgcc aagtatttaa caagactcta ataaaaccct gcaattggct
     9301 ctaacaaaac ctgcaactaa taattttgat attgatatta agattattat tgatattaac
     9361 attaagataa ggagttatat ttatgaaagt gttatttata ggtggtacgg gtacaataag
     9421 tactgctgta tctgaacttg cagtgaaaaa gggaattgat ttattcctct tgaacagggg
     9481 aaatcgtggg gaattttttc ctgaaggggc tactctgatt agaggagata tcaggaatat
     9541 agaaacaata cataaaatat tggatgatta taaatttgat gttgtggttg actgggtagc
     9601 atttactccg gaccacatcg aagcagacat aggtcttttc aaaggaaaga tagggcagta
     9661 catatttata agttcagcat cagcatatca gagaccaccg gcccattacc tggttgatga
     9721 at
//
LOCUS       DAOSDD010000177         9613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2660_length_9613_cov_4.777255, whole genome shotgun sequence.
ACCESSION   DAOSDD010000177 DAOSDD010000000
VERSION     DAOSDD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_9613_cov_4.777255"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..722
                     /locus_tag="PK733_14045"
     CDS             24..722
                     /locus_tag="PK733_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOJ11700.1"
                     /translation="MTQVTDENKEVIITKNNVRVARLVPYVSDIEKYFTVRENAVNYD
                     YHRKTVSYEEFMEIYENTSTRMEFINGEIFIMGSPSFSHQSILGDLHVTFKEYFRGKR
                     CKPIIAPFDVHFKKQDIKDPDVMQPDLVVLCDLENNINEKGRYMGTPTLTLEILSPST
                     RSIDMLYKLNTFMTSGVSEYWLVDPDNQMITVYTFTDYQIDKMKVYKKEETAKSEIFE
                     GLSVDVKLLFDEMF"
     gene            complement(892..1854)
                     /locus_tag="PK733_14050"
     CDS             complement(892..1854)
                     /locus_tag="PK733_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11701.1"
                     /translation="MFANLDSLNLKTKIGGLVFDIILDKRFFTPDMDNVVHKHKHSLY
                     EFHYIAEGEGTVYINNSEYKVYPGCYFIVNSGVYHKQLQDNNNPIHKHCFKFEYKYQS
                     HLADSFFPEEESKRIADILNQLDYFISTQNIVNDTTNHINNLQSFSHDSSNNSGKIIS
                     LIHDIQEELSSKKVGFYARIQCLFTQLFIFIVRDITLDKETNYSIPQKLPYEKRSALI
                     EGFFDTHFNDNVTSDDLCKLLNLSTSQLNRILKKKYNSTFGQKLLEMRIEAAKRYLET
                     TSFPISIIAEKVGYSSAAHFCNIFKDKCGITPGEYRASMSRKIQ"
     gene            2231..3121
                     /locus_tag="PK733_14055"
     CDS             2231..3121
                     /locus_tag="PK733_14055"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ11702.1"
                     /translation="MMSNKVRNGFYTALGTPVDSYGNLVKSSMEKHIIDQVEYNASGL
                     LVMGSMGLEPYIKQSEYAEVAKMSAEAAKGKCTVFVGAMDNSVVRVEERIKSLEGLKI
                     DGVVLTTPFYMGTSQEELKSYFTKIAAISPFPVYLYDLPGITKTKINADTVEYLMDND
                     NIKGIKTGDILTARVLMRSEKKKDDFSVLFSGLDIFDVAYKYGIKMNLDGMFSCTHPI
                     ADKMYRLMEQGDYCGGAACLDQILTLRNLFIKVGVMRGFSYAMNLMGYEGSFCPDYTV
                     AGKEEEFACIKEFLKEHKLI"
     gene            3221..3655
                     /locus_tag="PK733_14060"
     CDS             3221..3655
                     /locus_tag="PK733_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11703.1"
                     /translation="MAPIKEWFGLYNVIYNWFEKNYGYEALEEYWMFIAENCFEDVVE
                     KFKDEGLDGIRGYYEHIFNLDEGECKTYLENGKLTIEVNKCPDYEFMKSSPNPHFVPI
                     KSYCKHHEVMNNILADKSGHTFCMCQCDNNGHCKWMFTKKEV"
     gene            3659..4111
                     /locus_tag="PK733_14065"
     CDS             3659..4111
                     /locus_tag="PK733_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11704.1"
                     /translation="MTVHDFIATYNEAFKYIEAKYGVEAVKDLWQTISEEWCTHLDEL
                     IKNKGMDGMVEYWGGEDGTLGRENAEYEISLKDGVFSGIMHECPSVKELHERNRKVYN
                     GKLTYCDNCPVLYAPVAKKYGIEMTFDIEHEEDGECTGRCKWSAAAHS"
     gene            complement(4117..5151)
                     /locus_tag="PK733_14070"
     CDS             complement(4117..5151)
                     /locus_tag="PK733_14070"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOJ11705.1"
                     /translation="MRPSSLRDKLLELYAEQEVAAQEEYDIDAVKRCVINYLRKKYDN
                     NKIQRVIDDMYSRYDLTADSKEIRRRNQLTEYIDSIVMSRIRDSYKSSKILDIDSLDE
                     LKFKALVKQVIGHFGYETLFVPVNSLNNIDIIVHRKDIKIAVLAIKGEPGSIIGLKTI
                     RRLRYIANYYHCEQALLITNTFFDSEAFNEAAEIGITLLDRDKLVPLVKDLIDGRQKE
                     DKEFLIDDIEDQKNSIFLEGEIKFPKTKVQVVFVKYYIDNDTNYLTFEGKLVNTGKKP
                     ASNISVEIKLFNRNNDSIYSKSFPIEKEKLESKEEAAFKFYFNEIPQHDWENLCRYQL
                     KLEYKNTYKE"
     gene            complement(5170..7149)
                     /locus_tag="PK733_14075"
     CDS             complement(5170..7149)
                     /locus_tag="PK733_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11706.1"
                     /translation="MGSVYSSELHKFVESYFNTIGAYISEKQENFFIVKFPDGLKETY
                     TYVARVAAENKDIKLLARGSSSLNNMIKECSLKAGFSEVDAIYTEESVKSTLAHKNCC
                     DLCPFFTICDNKDKCCDFCPYYKSCNTRLLNAEFIKLGDVKESIPLDVLCFIFLVELS
                     NDYSLSQKVEKFVSVLIDMNTGKTIGDICFNGLAGIEMMQSSKYISISEKDYQQFMNI
                     ARQEAFEAVKNQLEVFKKEIEDTLKDKIRAIVDKYEEEYIENYTRSTLEQLEKFQDEG
                     LKLCEREIRGYAINCDYHLKNVILLHTVKDLRNLIFKLHDNSHEVSIPTEIFLNRVDI
                     RCAECNAEIDSGAICSNGHAACKNCIDMCIACGKPICNVCDDESYICSTCGEIVCTSC
                     MSQCASCGAVVCPAHSYRCVTCGNTFCIDCYEICNICGSSVCANHAGTCSSCGAYVCS
                     DHMHKCTVCNTAYCDEHIYNCFICNDKLCEEHALKSAFSGKTICTRHSGKCGKCEGVF
                     ALDELKKCALCGIGLCPEHIKICSNCEKVYCEEHINHCKSCGRDYCSCTQSVKCKLCG
                     GTYCPDCVDSKGLCKACEGLAYVDRSDEKIQSILELVPDIAKCRKFYLGRANEINVLY
                     AKNLISGYLAVFNNNNEILSNRKINFIEFLKTKFI"
     gene            complement(7162..9180)
                     /locus_tag="PK733_14080"
     CDS             complement(7162..9180)
                     /locus_tag="PK733_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOJ11707.1"
                     /translation="MQEVVPRFTNQNLIKIKQRSNDAGLLIDLVPVHNSYSDDNKIAV
                     RIKDGDCNRSADWLKLGLMAADLMKTGKIDSLSTLDLLDGRFEPYPYQLKVALNILKE
                     KSSVAMLADEVGLGKTIEVGLILKEHIVRDNVHSILIITPKALVLQWKDELNEKFGEE
                     FLTTEDDNFDYSSNRIITSFNKLSRNVDKFTNRQWDMIVVDEAHLLINASSKRRQAVG
                     SIDRRYMLLCTATPLCNKLTDIYSLVDLLYPGILGTEKSFKNKYFADRNGRVCRIEMK
                     DDLKKIVSKVMVRTLRKDSGIPFTERYVYSFRINGSREEALLYDMVIRFMQSIYENNL
                     SKLTNSGNNETRNSFLGLEGSRLLDRSKVVQSSRTVQSGRSVQGSYVIQGLQGLQGLQ
                     GVRSVLTSPPKTQNKSKVNYLLMKELISMQQSLSSSPRALIKTLENRRDKYPHEIPMI
                     DPIISLASEIGIYSKVQKLLETLNSIPGEQGIIFTLRLETAYMLCDSLNENYMTARVY
                     EGRLSGYERQALIDEFRRGKVQYIVATDTAAEGLNLQNATIVVNYDLHWNPMKIEQRI
                     GRIHRRGQNKDVNIFNLVMKDTIDDYVLKVLYEKIDLFKMTIGGIESILSEIRDDDFS
                     IEETIMDIIMRSSKKRDIEKELDELRESMEYVKEQSMLREEFSRGILD"
     gene            9471..>9613
                     /locus_tag="PK733_14085"
     CDS             9471..>9613
                     /locus_tag="PK733_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOJ11708.1"
                     /translation="MKLLLHICCAPCSVYPTGLFKEEDINFEGLYFNPNIHPIEEFNR
                     RKET"
BASE COUNT         3009 a         1730 c         1672 g         3202 t
ORIGIN      
        1 tgagaaataa ttttagtcat tatttgacac aggttacaga tgaaaataag gaggttatta
       61 ttaccaaaaa caatgtaaga gtagcaaggc ttgtgcctta tgtatccgat attgagaagt
      121 attttactgt acgtgaaaac gcggtaaatt atgattatca cagaaaaaca gtttcatatg
      181 aagaatttat ggagatatat gaaaatacca gcaccaggat ggaatttatt aatggtgaaa
      241 tattcattat gggttcaccg agtttttctc atcaatctat tttaggcgat ttacatgtca
      301 cttttaaaga gtattttagg ggaaaaagat gtaagccaat tattgctcct tttgatgtgc
      361 atttcaaaaa acaggatata aaagatcccg atgttatgca gcctgattta gttgtgttgt
      421 gtgatctgga gaataacatt aatgaaaaag gcagatatat gggaactccc acattaacat
      481 tggaaattct atctccaagc accagaagca tagatatgct atataagctt aatactttta
      541 tgacctcagg agtgtctgaa tattggctgg ttgatcctga taaccaaatg attacagtat
      601 atactttcac tgattatcaa attgataaaa tgaaagtcta taagaaagag gaaactgcca
      661 aatcggaaat atttgaaggg ctatctgtgg atgttaagct attatttgat gaaatgtttt
      721 gattagaggt gcatacctgc acctaaactc agcatgactc aatgtcgtgc tgagtttgat
      781 taaatgtgcc gcgcatcctt gcatcctaaa caagccattt tccgcagcat tggtaaccct
      841 atgtttatcc aggcgataac ttggaatatt aactgcaaag tgtgcagatt tctattgaat
      901 ttttctactc attgaagctc tgtattcacc tggggttata ccgcatttat ccttgaaaat
      961 attacaaaag tgagcagcag atgaataccc tactttttct gcaataatgc ttattggaaa
     1021 gctcgttgtt tcaagatatc tttttgcagc ttcaatacgc atttccagta gtttttgtcc
     1081 gaatgttgaa ttgtatttct ttttcaatat tctgtttagc tgactggtac ttaaattcaa
     1141 taatttacat aaatcatctg atgtaacatt atcattaaaa tgtgtatcaa aaaacccttc
     1201 aatcagtgcg ctgcgctttt catatggcag tttctgaggt attgaatagt tggtttcctt
     1261 atctaaagtg atgtctctta cgataaagat aaatagttgt gtaaaaagac actggattct
     1321 tgcataaaag ccaacttttt tagatgatag ttcttcctga atatcatgaa taaggctaat
     1381 tattttccca gagttattac tgctgtcatg actaaaagat tgcaggttgt taatatgatt
     1441 agtggtatca tttactatat tctgtgtact gataaaataa tccagctgat ttagtatatc
     1501 tgcaattctc tttgactctt cctctggaaa aaaactatct gcaagatgag actggtattt
     1561 atattcaaac ttaaagcagt gcttatgtat gggattgtta ttatcctgca attgtttatg
     1621 ataaacgccg gaattaacta taaagtagca gccgggatat actttatact cactattatt
     1681 tatatagaca gtaccttcac cttcggcaat gtaatgaaat tcatataagg agtgtttatg
     1741 tttgtgtact acattatcca tatccggggt aaaaaacctt ttatccagta taatatcaaa
     1801 tacaagtccc cctattttgg tctttaggtt taaactatcc aggtttgcga acatggttat
     1861 accgtcctta tctcttattt ttagtaatag ctgctatata ctattatatt ataattatac
     1921 actatattta agggcacgat atcctgggga tatcacctat gggtgtaatt ttaccataaa
     1981 aaaggtatat tgtaatagta ccctccagaa atgcgctttc cattagcaac ataacaaagt
     2041 agtattaagt aattgcgcta aatcgtcatt ttcatataaa agaatgaatc attatagcat
     2101 tttaagtacg caacaaatta gatataatta cgttatattt ataattgtgt gtattaagca
     2161 cattcacaaa acaacttaaa aagctcgtaa aaagataaaa ttatatgaac ttctttgaaa
     2221 ggggagtcta atgatgtcaa ataaagtgag aaacggtttt tatactgcgt tgggtactcc
     2281 ggtagattca tatggaaatc tggttaaatc cagtatggaa aaacatataa tagaccaggt
     2341 tgaatataat gcttccgggc tgctggttat gggaagcatg ggacttgaac cgtatatcaa
     2401 gcaatctgag tatgctgaag ttgcaaaaat gtcagcagaa gccgcaaaag gtaagtgcac
     2461 tgttttcgtt ggagccatgg acaattccgt tgtcagggta gaggaaagaa taaaaagtct
     2521 tgaagggcta aagatagatg gtgttgtttt aacaactcct ttctatatgg gtaccagcca
     2581 ggaagaatta aaatcttatt ttacgaagat agctgcaatc tcaccttttc cggtatactt
     2641 atatgatctt cccggcatca caaaaactaa aataaatgca gatacggtag agtatcttat
     2701 ggataatgat aatataaaag gtataaagac aggagatatc cttactgcaa gggttcttat
     2761 gcgcagtgag aaaaagaagg atgatttcag tgtgctgttc agcggcctgg atattttcga
     2821 tgttgcttat aaatatggta taaaaatgaa tttggatgga atgttcagct gtactcatcc
     2881 tattgcagat aaaatgtata gacttatgga acaaggagat tactgcggcg gtgcagcgtg
     2941 ccttgatcaa atacttaccc tcagaaacct gtttatcaaa gttggtgtaa tgagaggatt
     3001 tagttatgca atgaacttga tgggatatga agggtctttt tgccctgatt atactgttgc
     3061 aggtaaagaa gaagaatttg cctgcattaa ggagtttttg aaggaacata agcttatctg
     3121 agcttttagc ttttgaagta tggttccaaa attatttgga gactgttctc tataaaaaga
     3181 aggactgtcc ccatagcaaa taaatggaag gtgaattaac atggctccta ttaaagagtg
     3241 gtttggactc tataacgtca tatataactg gtttgaaaag aattatggat atgaagcact
     3301 ggaagagtat tggatgttta tagctgaaaa ctgttttgag gatgtagttg aaaaattcaa
     3361 agatgaaggg cttgatggta taagaggtta ttatgaacat atttttaatt tggatgaagg
     3421 cgaatgtaaa acatatctcg aaaacggaaa gcttacaatt gaagttaaca agtgcccgga
     3481 ttatgagttt atgaagtctt ctcccaatcc tcattttgtt cccattaaaa gctactgcaa
     3541 acaccatgag gttatgaata atattctcgc agataaaagc gggcatactt tctgcatgtg
     3601 tcaatgtgac aataatggtc attgcaaatg gatgtttaca aagaaagagg tgtaaaatat
     3661 gacagttcat gattttattg caacctataa cgaggctttt aagtatatag aagcaaaata
     3721 tggtgtagag gctgttaaag atttatggca aactatctct gaagaatggt gcactcattt
     3781 agatgagctt ataaaaaaca aaggtatgga tggaatggta gagtattggg gcggggaaga
     3841 cggcaccttg ggaagagaaa atgcggaata tgagataagt ctgaaggatg gagtcttttc
     3901 aggtataatg catgaatgcc catcggtaaa ggagttacat gaaagaaata gaaaggtcta
     3961 taacggcaaa ttaacttatt gtgacaattg ccccgtatta tatgcccctg tcgcaaagaa
     4021 atatggaata gaaatgacat ttgacattga gcatgaagag gatggtgaat gcacaggaag
     4081 gtgtaaatgg agtgcggcag ctcactctta agcgactcac tccttataag tatttttata
     4141 ctccagtttt agctggtacc tgcatagatt ttcccaatca tgctgaggta tttcgttaaa
     4201 ataaaacttg aaggcagctt cttccttgct ttcaagtttt tctttttcaa ttggaaagct
     4261 tttcgaatag atgctgtcat tgtttctgtt gaaaagtttt atttccacac ttatatttga
     4321 agcaggcttt ttgccagtgt taaccagttt accttcaaat gtgaggtaat tggtatcatt
     4381 atcaatataa tactttacaa atacgacctg taccttggtt ttggggaatt ttatctctcc
     4441 ctccaagaat atggagtttt tttggtcttc aatatcgtcg attaaaaatt ccttatcttc
     4501 cttttgacga ccgtcaatca aatccttcac taaaggaaca agtttatccc ggtcaagaag
     4561 ggtaatacct atttccgctg cttcattaaa agcttccgaa tcaaaaaagg tatttgtaat
     4621 taacaatgct tgctcacaat ggtaatagtt ggctatatat ctaagccgtc tgattgtctt
     4681 aagtccgata atgctgcccg gttcaccttt tatagctaaa acagcaattt ttatatcttt
     4741 gcgatgaact attatatcaa tattatttaa actgttaaca ggaacaaaaa gtgtctcata
     4801 tccaaaatgc ccaattacct gttttaccag ggccttaaat ttcaactcgt caagagaatc
     4861 aatatccaga attttggatg atttatatga gtccctgatt ctgctcatga caatagaatc
     4921 tatgtattca gtaagttggt ttcttcgcct tatttcttta gagtcggcag taaggtcata
     4981 tctggagtac atatcgtcaa tgaccctttg aatcttatta ttatcgtatt ttttgcgaag
     5041 gtaattgata acacaccttt tgacagcgtc aatatcatat tcttcctgcg cggcaacttc
     5101 ctgttcagcg tataattcaa gtaacttatc acgtaacgac gaaggtctca tagtattcgg
     5161 caactcctac tatataaatt ttgtctttaa aaattcaata aaattaattt tcctgttgga
     5221 cagtatctcg ttattattgt taaacactgc cagataccca cttatcaggt tctttgcata
     5281 taaaacattt atttcatttg ctctgcccag atagaatttt ctacactttg caatatcagg
     5341 tacaagttca aggatgctct gaatcttttc atcgcttcta tcaacatagg ccaggccttc
     5401 acatgccttg cacaggcctt tgctgtcaac acaatcagga caatatgttc ctccgcagag
     5461 cttgcattta acactttgcg tgcaactaca gtagtcacgg ccgcaggatt tacagtgatt
     5521 aatatgttct tcacagtaaa ctttttcaca attactgcaa atttttatgt gttcaggaca
     5581 gaggccaata ccgcaaagag cacatttttt caattcatca agagcaaaaa cgccttcaca
     5641 ttttccgcat tttcctgaat gtctggtgca gatggttttt ccgctgaatg cagactttag
     5701 tgcatgttct tcacatagct tatcattgca gatgaaacaa ttatatatat gttcgtcaca
     5761 ataagcagta ttacatacgg tgcatttatg catatgatcc gagcatacat aggcaccgca
     5821 gctgctgcag gtgccggcat gattagcgca gacgctgctc ccacaaatat tgcatatttc
     5881 ataacagtct atgcagaagg tattcccaca ggtcacacag cgataactat gagctggaca
     5941 tactacagct ccacatgaag cacactgtga catgcaacta gtacatacaa tctcgccgca
     6001 ggttgagcaa atgtagcttt catcatcaca tacattacat ataggtttgc cgcaggcaat
     6061 acacatatca atacagtttt tgcatgcagc atgtccgttg ctgcaaatag cacctgaatc
     6121 aatttcagcg ttacattccg cacatcgtat atccactctg tttagaaata tttctgtagg
     6181 tatactaact tcatggctgt tatcatgaag tttaaatata aggtttctta aatcctttac
     6241 tgtatgcaat aatattacat tcttaagatg ataatcacag ttaatagcat aacctcgtat
     6301 ctccctttca catagtttaa gtccctcatc ctgaaacttt tcaagttgct caagagtact
     6361 tctcgtgtaa ttttcaatat actcttcttc atatttgtca acaattgcac ggattttatc
     6421 cttaagggta tcttcaattt ccttcttaaa tacttccagt tggtttttta ccgcttcgaa
     6481 ggcctcctgc ctcgcgatat tcataaattg ctggtaatcc ttttcactta ttgaaatgta
     6541 tttggaggac tgcatcattt caatacctgc aagcccatta aaacaaatat cacctatagt
     6601 tttacctgta ttcatatcaa ttagaacaga aacaaatttt tccactttct ggcttaagct
     6661 atagtcattg gaaagctcta ctaaaaatat aaaacataaa acatccagag gaattgattc
     6721 tttaacatcc ccaagtttta taaactcagc attaagcaat ctggtattac atgatttata
     6781 gtaaggacaa aaatcacagc atttatcctt attatcacaa atagtaaaaa aaggacataa
     6841 atcacaacag ttcttatgag caagagtaga ttttacagat tcttcagtgt aaatcgcatc
     6901 aacttctgaa aaaccggctt ttaaggaaca ttcttttatc atattgttta aagaggaact
     6961 tcccctggca agaagcttta tatccttatt ctcagcggca actctggcaa cataagtata
     7021 tgtttccttt aaaccatccg gaaacttaac tataaaaaag ttttcctgct tttctgaaat
     7081 atatgcacct attgtattga agtagctttc aacaaatttg tgaagctccg agctataaac
     7141 cgaacccata attcctccgt actaatccag aatacctctg gaaaattcct ctcttagcat
     7201 actctgttcc tttacatatt ccatactttc tctaagttca tcaagttcct tttcaatatc
     7261 cctcttttta gatgaacgca taattatatc cattatagtt tcctcaatgc tgaagtcgtc
     7321 atcccttatt tctgataata ttgactcaat tcccccaata gtcattttaa aaaggtcgat
     7381 tttttcatat agaactttca gaacataatc atctattgta tctttcataa ccaggttaaa
     7441 gatattaaca tctttgtttt gacctcttct gtgtattctt ccaattctct gctctatttt
     7501 catagggttc caatgaaggt catagtttac aacaatagtg gcattctgca ggtttaaacc
     7561 ttccgctgca gtatcggttg ctacaatata ctggaccttg cctcgcctga attcatcaat
     7621 caaagcctgt ctttcatatc ccgacaatct gccctcataa actcttgcag tcatatagtt
     7681 ttcatttaaa ctgtcacaaa gcatatatgc tgtttcaagt cttaatgtaa atatgattcc
     7741 ctgttcaccg ggaatactat taagagtttc aaggagcttc tgcactttgg aataaatacc
     7801 tatttccgat gcaaggctta ttatgggatc aatcatagga atctcatggg gatatttgtc
     7861 cctcctgttt tcaagcgttt taatcaaagc tcttgggctg ctggacagag attgttgcat
     7921 agaaataagc tccttcatta ggaggtaatt aaccttgctc ttattttgag ttttgggtgg
     7981 ggaggtcaga acactgcgca ctccctgcag tccctgtagt ccctgtagac cctgtataac
     8041 atagctgccc tgaactgacc ggccgctctg aaccgtccgg ctgctttgaa ctaccttgct
     8101 tctatcaagt aacctgctcc cttcaagacc tagaaagcta ttccgggttt cattattacc
     8161 actatttgta agcttactta aattattttc ataaatactt tgcataaacc tgataaccat
     8221 atcatataac agggcttcct cccgagaacc atttattctg aaagaataaa catatctttc
     8281 agtaaagggt atgccactgt cttttctcaa tgtccttacc attacttttg aaactatttt
     8341 ctttaaatca tctttcattt ctatacggca tactcttcca ttccggtctg caaagtattt
     8401 attcttgaaa cttttctcag tacccagaat tccggggtat agtaaatcta caaggctata
     8461 aatatcagta agtttattgc ataaaggtgt agcagtgcaa agcagcatat atctccggtc
     8521 aatagagcct actgcctgcc ttcttttaga actggcattt ataagcagat gggcttcatc
     8581 cactacaatc atatcccatt gcctgtttgt gaacttatca acatttcttg ataatttatt
     8641 aaatgatgtt ataatcctgt tactgctata atcaaaatta tcatcctcag tagtcagaaa
     8701 ctcttcgccg aatttttcgt ttagttcatc cttccactgg agaacaagcg ctttaggggt
     8761 tattataagt attgaatgaa cattatctct tacgatatgc tctttcaaaa tgaggcctac
     8821 ctcaatagtt ttaccaagac ctacttcgtc agcaagcatg gcaacacttg atttttcctt
     8881 aagaatattt agtgctactt ttagctgata aggataaggc tcaaatctcc catcaagaag
     8941 atcaagagta gatagactgt caattttacc cgtcttcata agatcggcag ccattaagcc
     9001 aagctttagc cagtcagcgg aacgattgca atctccatct ttaatcctga cagcaatttt
     9061 gttgtcatca gagtatgagt tgtgaaccgg tactagatct ataagaagac ctgcatcatt
     9121 actcctctgc tttattttta ttaaattttg attagtaaac cttggtacaa cctcctgcat
     9181 aaaatacccg ccccaaataa aaattctgta tgcttttgtc gaaataattt tgtattctat
     9241 atatttctat atatttatat tttaacattt gaaatcattt aattaattag tatattagac
     9301 tgatttaaaa taagtttttg tacatattat acaaaaactg tgtattttta aggttttatg
     9361 ctgtggacag gtatatatcc tgtgctaatt ttggtgtata atacagtaag aaaaatgcgc
     9421 tcgaaaaatg ctgatattaa gttatttgac gaaaggagta aaataaaaaa atgaaactgt
     9481 tattgcatat atgctgtgca ccttgttcgg tatatccaac aggtcttttt aaagaggagg
     9541 atataaattt tgagggttta tattttaacc ctaatataca tcccattgaa gaatttaata
     9601 ggagaaaaga aac
//
LOCUS       DAOSDD010000178         9600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2665_length_9600_cov_5.149293, whole genome shotgun sequence.
ACCESSION   DAOSDD010000178 DAOSDD010000000
VERSION     DAOSDD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2665_length_9600_cov_5.149293"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..1897)
                     /gene="gpmI"
                     /locus_tag="PK733_14090"
     CDS             complement(356..1897)
                     /gene="gpmI"
                     /locus_tag="PK733_14090"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ11709.1"
                     /translation="MKDRLVALIILDGFGVNPKDEGNAIKAACKPNLDKYFKEYPNTT
                     IKCSGMDVGLPNGQMGNSEVGHTNIGAGRIVYQELTRITKSVEDEDFFMKKEFLDSIE
                     NCKTNNSKLHLFGLLSDGGVHSHNTHLYALLELAKKHNLKDVFVHCFFDGRDVPPDSA
                     LGYVEELEKKIDEIGAGKIASVMGRYYAMDRDNRWERVNLAYNAMVLGEGLIAQSARQ
                     AVLDSYSKNEFDEFVKPTVVYENNKPVATIGDNDSIIFFNFRPDRAREITRAFIDKDF
                     SGFQRANGYFPVHFVCMTQYDKTMENVEVAFKPESLKNTFGEYISGLGYRQLRIAETE
                     KYAHVTFFFNGGVEKVYDGEDRALIPSPKVPTYDLKPEMSAYEVTDEVVKRIASKQYD
                     VIILNFANADMVGHTGIFTAARAAIEAVDACLGRIIPAIIEQGGVALITADHGNAEQM
                     VDYDTGGPFTAHTINSVPLICAGLGDVKLKEGRLADLSPTLLDIMGIEKPAEMTGISL
                     LEAKR"
     gene            complement(2089..4035)
                     /gene="tpiA"
                     /locus_tag="PK733_14095"
     CDS             complement(2089..4035)
                     /gene="tpiA"
                     /locus_tag="PK733_14095"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF010569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ11710.1"
                     /translation="MSMINKKTVEDIDVSGKKVLVRVDFNVPQDDNGNITDDKRIVAA
                     LPTIKYLINKGAKTILVSHLGRPKSGFEDKYSMKPTAIRLSELLGKEVIMAKDVIGED
                     AKAKAEALKPGDVLLLENVRFHKEEEKNNPDFAEALANLAEIYVNDAFGTAHRAHAST
                     AGVADYLPAVCGFLIKKEIEIMGKALGNPARPFIAILGGAKVSDKIGVIENLLDKVDA
                     LIIGGGMAYTFLKAKGYKIGNSICEEDKLDLARSLMEKAENKGVELVLPIGSIVGKEF
                     KNDTEHKYVPSDDMPDGWMGMDIGSLTIEKFTDVIKKAKTIVWNGPMGVFEFPNFATG
                     TKEIARAVAESGAISIVGGGDSAAAVEQLGFADKITHISTGGGASLEFLEGKELPGIA
                     VLCDKNPRKKVIAGNWKMNKTAKEAVDFVANLKDRVAGSADEVVVCVPFVCIPGVKQA
                     AEGSNIKVGAQNMHWEEKGAFTGEVSGPMLKDLGVEYVIIGHSERRQYFAETDETVNK
                     KVHAAFKYGLKPIICVGESLTQREQGVTADLVRYQVKIALLGLTCDQVKNVVMAYEPI
                     WAIGTGKTATNEQADEVCAIIRDTIKELYGEETSEAVRVQYGGSVNAANASELFSMVN
                     IDGGLVGGASLKLDDFEKIVKYDK"
     gene            complement(4224..5234)
                     /gene="gap"
                     /locus_tag="PK733_14100"
     CDS             complement(4224..5234)
                     /gene="gap"
                     /locus_tag="PK733_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ11711.1"
                     /translation="MSVKIGINGFGRIGRLVFRAAIDNPKVEIKGINDPFIDLDYMVY
                     MLRYDTVHGQFPGTIEVEDGNLIVNGNKITVYNFMNPSEIPWSACGAEYIVESAGVFT
                     TTEKASAHFQGGAKKVVISAPSSDAPMFVMGVNHDKYTKDMNVVSNASCTTNCLAPLA
                     KVIHDNFGIVEGLMTTVHSTTATQKTVDGPSKKDWRGGRAASGNIIPSSTGAAKAVGK
                     VIPELNGKLTGMSFRVPTLDVSVVDLTCRLEKSATYDEIKAAVKKASENELKGILGYT
                     EDDVVSSDFIHDPRTSIFDAKAGIPLNSNFVKLVSWYDNEWGYSNKVVDLIVHMATVD
                     AK"
     gene            complement(5533..6660)
                     /locus_tag="PK733_14105"
     CDS             complement(5533..6660)
                     /locus_tag="PK733_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ11712.1"
                     /translation="MQYRKFGNTGVMISALGFGSMRLPEIEKDGQRVVDEENSIKIIH
                     RAFELGVNYIDTAYGYCGGRSENVVGKALKGYRDRVYLSTKMPTWRAKQHGDYRRLLE
                     EQLKKLDVDYIDFYHFHSLSKDSWENTVLKHNLVEEAQQAKDEGLIKHISFSFHDKPE
                     VLMQLVDTGIFESVLCQYNLLDRSNEEAIAYAKSKGLGTVVMGPVGGGRLASPSEIIA
                     RSVGGKAKSTPEVALRFVLANSNVNCALSGMSTIEMVEENVKVASIEEPLNNEDWDRI
                     NRTFEENRKLADLYCTGCEYCLPCPKGINIPRVFNIMNYYQVYGLKDYAFKEYEQVGK
                     EENYGSSPADCIECGQCEKKCPQHIKIREQLKKVQKGFSKI"
     gene            complement(6828..7913)
                     /locus_tag="PK733_14110"
     CDS             complement(6828..7913)
                     /locus_tag="PK733_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ11713.1"
                     /translation="MTKRIGVLTGGGDCPGLNAVLRAVAKTAMFKYGWEVIGFKDGYR
                     GLVLNDYMIFGPGDASGILDKGGTILGTSNRDNPFKFRVEVNGKVEYKDMSEQVINNI
                     NSLGIDCMIMIGGDGTLTSARDFSRKGLKVIGVPKTIDNDLSGTDYTFGFMTAVETAT
                     EAIDKLHSTAESHHRVMILEVMGRYAGWIAIESGIAGGADVILIPEIPYNINKVADKV
                     LERKKAGKHFSIIVVAEGAKSADGDIVVSKVLKDSPDPIRLGGIGNKIAEEIETLTGV
                     ETRVTILGHLQRGGRPIPYDRILSTRYGVAAVEMANAGKFGTMVSLKGSNITEVSLEE
                     AVGATKTVPPDGELVRIAKSIGVSFGG"
     gene            complement(7961..8452)
                     /gene="trmL"
                     /locus_tag="PK733_14115"
     CDS             complement(7961..8452)
                     /gene="trmL"
                     /locus_tag="PK733_14115"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOJ11714.1"
                     /translation="MSLNIVLVEPEIPQNTGNIARTCAATGTALHLVRPLGFSLEDKY
                     LKRAGLDYWNKLEILYYDSFEELRRKYPSNSFYYSTTKAAAVYTDFIYPEDCFIVFGK
                     ETAGLPEDLLKANKDWCVRIPMRETIRSLNLANSVAIVLYEALRQNRFNGLKEFGKMH
                     LEC"
     gene            complement(9090..9266)
                     /locus_tag="PK733_14120"
     CDS             complement(9090..9266)
                     /locus_tag="PK733_14120"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11715.1"
                     /translation="MGIPTGEKSGWLVLDESEIKIIAHSVSRYRPNPPQKKYHHRTDS
                     GNAERLRDRFGSII"
BASE COUNT         2690 a         2202 c         1427 g         3281 t
ORIGIN      
        1 cttttttagg tacagatata ttttttcatt ctttttcatc tgagaatacc cccatatctt
       61 ataaaacacg ttacttaata tattaacctt atcttgtaaa aaagtaaaga gtttcataaa
      121 aggcagggtg catttactat atttgaagat acacttacga ggttggaagt gagatgtgag
      181 atgtgggaat aagagcagac aaaagcataa gtttcataag cccggcctgt aacttctaat
      241 ctctattctc taaccccata ttatccaatc tccatctcct gccttctacc tcttatctct
      301 aacctctcat ctctaacctc tcatctctaa cctataactt cttgcctatc atctcttacc
      361 tctttgcctc taataatgaa atacctgtca tttcagcagg tttttcaata cccattatgt
      421 caagtaaggt aggacttaaa tcagcaagtc ttccttcctt taatttgaca tcaccaagtc
      481 ctgcgcatat taaaggtaca ctatttattg tatgtgctgt aaaaggccct cctgtatcat
      541 agtcaaccat ttgctcagca tttccatggt cagctgtgat taacgcgact ccgccttgtt
      601 caataatagc aggtataatt ctccccagac atgcatcaac agcttctatg gcggctcttg
      661 cagctgtaaa aatacctgta tgccctacca tatctgcatt tgcaaaattt aaaataatca
      721 catcatattg ttttgatgct atccttttta ccacttcatc ggtaacctca taggcactca
      781 tttcaggctt caagtcatat gttggaactt tgggcgatgg tataagggct ctgtcctccc
      841 catcatatac tttttctacc ccgccattaa agaaaaatgt tacatgagca tatttttcag
      901 tttcagcaat ccttagctgt ctatatccca gcccgcttat atactctcca aaagtatttt
      961 taagactttc aggtttaaag gcaacttcca cattttccat tgttttgtca tattgtgtca
     1021 tacaaacaaa atgtacggga aaataaccat ttgccctctg aaaccctgaa aaatccttat
     1081 cgataaatgc tctggtaatt tccctagccc tatcaggtct aaaattgaag aatattattg
     1141 agtcattatc acctattgta gcaacaggtt tattgttttc ataaactact gtaggtttta
     1201 caaattcgtc aaattcattt tttgaataag aatctaaaac tgcctgcctg gcactttgag
     1261 caattaaacc ttcaccaagt accatggcat tgtaagcaag atttactctt tcccatctgt
     1321 tatctctgtc catagcatag tatcttccca taacagaggc aatttttccg gcccctattt
     1381 catcaatttt tttctcaagt tcctcaacat atccaagggc actgtcagga ggtacatctc
     1441 taccatcaaa aaagcagtga acaaatacat ccttcaggtt atgcttttta gcaagttcca
     1501 aaagtgcgta tagatgtgta ttatggctat gcacaccacc atcggaaagt aaaccgaata
     1561 agtgcagttt tgaattattt gttttacaat tctctataga atcaagaaat tcctttttca
     1621 taaaaaaatc ttcatcttct acggattttg tgattctcgt caactcctga tatactattc
     1681 tgccagctcc aatatttgta tgccctactt cggaattgcc catttgtcca ttaggcaatc
     1741 ctacatccat gccactgcat ttaatagttg tatttggata ttctttaaag tatttgtcca
     1801 gatttggttt gcatgcagct tttattgcat ttccttcatc tttcggatta actccaaatc
     1861 catcaagtat aattagtgca accagtctat ctttcattag tttttccact ccttgtagaa
     1921 tgatttttgc tatatgctat ataaggtaca taattcaatt tctaaaaacc caattggcca
     1981 gtttgttttt gattatgcct aatacaaaat tagggcaggt aaaagagcct gccctaatta
     2041 agccagcatt cacaggaaag aattatattg tgcttcctgt tttctgtttt atttatcgta
     2101 cttaactatt ttctcaaaat catcaagctt aagacttgct cctcctacta ggccgccatc
     2161 aatattaacc atgctaaata attctgaagc attggctgca tttacgctgc cgccatattg
     2221 tactcttaca gcctcagagg tttcttctcc ataaagctct tttattgtat ctcttattat
     2281 tgcacatact tcatcagctt gttcattagt ggctgttttc ccagtcccaa tagcccatat
     2341 tggttcgtaa gccataacaa cattttttac ctgatcacaa gttaaaccca ataaagcaat
     2401 tttaacctgg tatcttacaa gatctgctgt aacaccctgt tctctctgag ttaaggattc
     2461 accaacacag attattggtt tcaagccata tttaaatgca gcatgcacct tcttgttaac
     2521 tgtttcatca gtttcagcaa aatattgtct tctctccgag tgaccaataa tgacatattc
     2581 aacccctaaa tctttaagca ttggtcctga aacctcaccg gtaaatgcac ctttttcttc
     2641 ccaatgcatg ttttgtgctc caacttttat atttgagcct tctgctgcct gttttacgcc
     2701 cggtatgcat acaaaaggta cacatactac tacttcatca gcactgccgg ccaccctgtc
     2761 tttcagatta gcgacaaaat caacagcttc tttagcagtt ttattcattt tccagttgcc
     2821 tgctataact tttttccttg gatttttatc acataatact gctataccgg gaagctcttt
     2881 tccttcaagg aattcaagag atgcgcctcc gccggttgat atatgggtaa ttttatctgc
     2941 aaacccgagc tgctcaactg ctgcagctga atcgcccccg cccactattg aaatagctcc
     3001 tgattcagct actgctcttg caatttcttt tgtacctgta gcaaaattag ggaattcaaa
     3061 tacacccata ggtccattcc atactatggt ttttgccttt ttaataacat cagtaaactt
     3121 ctctattgta agagaaccaa tatccatacc catccaacca tccggcatgt catctgaagg
     3181 cacatactta tgttctgtat catttttaaa ttctttgcct actatactgc caatgggaag
     3241 aacaagttca acacccttat tttccgcctt ttccataagg cttcttgcaa gatcaagctt
     3301 atcttcttca catattgaat ttccgatttt atatcccttt gccttaagaa aggtatatgc
     3361 cattccgcca ccaatgatga gagcatcaac cttatcaagg aggttttcaa taacaccaat
     3421 tttatctgat accttggctc ctcccaatat tgcaataaaa ggacgggccg gatttcccag
     3481 cgctttaccc attatttcaa tttccttttt aattaaaaaa ccgcacactg ccggcaaata
     3541 atctgctaca cctgcagttg acgcatgagc tctatgagct gtaccaaaag cgtcattaac
     3601 atatatttct gcaagattgg caagggcttc tgcaaaatca gggttgtttt tttcttcctc
     3661 tttgtgaaac cttacgtttt caagcagcag gacatctcca ggtttaagtg cttcagcttt
     3721 agctttagca tcctctccaa ttacatcctt ggccattatt acttccttgc ccagaagttc
     3781 actaagccta attgctgtgg gtttcatgct gtacttatct tcgaaccccg actttggcct
     3841 tccgaggtga gaaaccagta tagttttcgc acctttatta attaaatatt ttattgtagg
     3901 gagtgcagca actatccttt tatcatcagt gatattcccg ttatcatcct ggggtacatt
     3961 gaagtcaacc cttaccagaa cctttttccc gcttacatca atatcttcta ccgttttttt
     4021 gtttatcatg ctcatataaa cacactccta tataaagatt aataatatta atttgctctt
     4081 aatatatgta aataattaca ccattcatcc aacattcaac attttacatc tttttccctc
     4141 aaatttcaat acttaaacaa aaagaggtat aataaaaggc ccggggttta attttccgga
     4201 cctttcatat gtttaatcaa tttttatttt gcatcaacag ttgccatatg aacaataagg
     4261 tctactacct tgtttgaata accccattca ttgtcatacc atgatacaag ctttacaaaa
     4321 ttgctgttaa gcggtatacc tgcttttgca tcaaatattg atgtacgtgg gtcatgaata
     4381 aagtctgaag atacaacatc atcttcagtg tatccaagga ttccctttaa ttcattttca
     4441 gaagcctttt taacagcagc tttaatctca tcatatgttg cagatttttc aagacggcag
     4501 gttaagtcaa ctacagaaac atccaatgta ggaactctga aagacatacc tgttaatttt
     4561 ccattcaatt ccggaattac ttttcctact gcttttgctg cacctgtaga tgaagggata
     4621 atgtttccgg aagctgcacg tccgcctctc cagtctttct ttgaaggacc gtcaactgtt
     4681 ttttgtgttg ctgttgtgga atgtactgtt gtcatcaagc cttcaacaat accaaaatta
     4741 tcatgaataa ctttggcaag aggtgcgaga cagtttgttg tgcatgatgc gttggatact
     4801 acgttcatat ctttggtata cttatcatga tttacgccca taacaaacat aggagcatca
     4861 gatgacggag cacttataac aactttttta gcaccaccct ggaagtgtgc agatgctttt
     4921 tcagttgttg tgaaaacgcc cgcagattca actatgtact ctgcgccgca tgcactccat
     4981 ggtatttcgg atggattcat aaaattataa acagtaatct tattaccatt tacaatcaaa
     5041 tttccatctt ctacttcaat agttcctgga aattgaccgt ggacggtatc atacctaagc
     5101 atatatacca tataatccag atcaataaaa ggatcattaa tacccttaat ttcaactttt
     5161 ggattatcaa tagcagctct gaatacgaga cgcccaatac gaccaaatcc attaatacct
     5221 atttttactg acatttaaac tacctcctaa aacaatttat tatattatta aatttagaat
     5281 tagcatctga aaccctgcaa ttttactatc aaagattgct accaatccat tacgatagtt
     5341 ttaggttagt ttcaaaactt attcagcaac aaaaaatcat gcaggattta cctgcatcaa
     5401 aaaatattct atagtatttg aaatattttt tcaatagtaa tactcaaaaa taattaattt
     5461 tttaacaata tattgcccca ataccgtaaa tttatgcgtt tccagcaaaa tcggttatat
     5521 ttttcgaatc agttatattt tgctgaatcc cttctgtact ttttttaact gttccctgat
     5581 ttttatatgt tgagggcatt ttttctcgca ttgtccacat tcaatgcaat ctgcgggcga
     5641 actaccatag ttctcctctt tccctacctg ttcatactct ttaaaagcat aatccttgag
     5701 gccataaacc tgataatagt tcattatgtt aaacactctt ggtatattta tacccttagg
     5761 acaaggcaga caatattcac agcctgtaca atacagatcg gccaactttc tgttctcttc
     5821 aaaagtcctg ttaattctgt cccagtcttc attgttcaaa ggttcttcaa tagaggcaac
     5881 tttaacattt tcctcaacca tttcaatagt gctcatacct gataaggcgc agttcacgtt
     5941 ggaatttgcc aaaacgaacc ttaaagctac ttcaggtgtg ctctttgctt taccccccac
     6001 tgaacgggca attatctctg aaggacttgc aagtcttcct ccccctaccg gtcccattac
     6061 tactgttccc agtcctttac ttttagcata agcaatggct tcttcgtttg atctgtcgag
     6121 caggttatac tggcacaaaa ccgattcaaa aatacctgta tcaaccagct gcattaatac
     6181 ttcaggttta tcatggaaag aaaaagatat atgttttata aggccttcgt ctttagcttg
     6241 ttgtgcttct tcaacaagat tatgcttaag cacggtgttt tcccaactat ccttgcttaa
     6301 gctgtggaaa tggtaaaaat caatataatc tacatccaat ttcttgagct gttcttccaa
     6361 gagtcttcta taatccccat gctgttttgc tctccatgtg ggcattttag ttgataagta
     6421 aactctgtcc ctgtagcctt ttaaagcttt tcctacaaca ttttcgcttc tccctccaca
     6481 gtaaccgtat gctgtatcaa tatagttaac tcccaattca aatgccctgt gtataatctt
     6541 tatggaattt tcttcatcta cgactctctg accatctttt tcaatctctg ggagacgcat
     6601 tgacccaaat cccagagctg aaatcattac acccgtattt ccaaatttcc tatattgcat
     6661 tacagaacct ccatgagtta tattattatt taatgaagct ggaaaccaaa agttgaagct
     6721 atcaaagacc caatcttaca tctccaacct ctaatcccca acctattacc tattacctat
     6781 gacctctagc atctcacatc tcgcatctaa cctctaacct ctaacctcta acctccaaac
     6841 gacaccccta tgctttttgc tattcttacc aactctccat caggtggtac agttttagtg
     6901 gcaccaactg cctcttccag cgaaacttca gttatattgc tgcccttaag actaaccatg
     6961 gtaccgaatt taccggcatt ggccatttca accgcggcaa cgccatatct ggttgaaagt
     7021 attctgtcgt aaggaatagg tcttcctcct ctttgaagat gtcccagaat agtaaccctt
     7081 gtttctacac ctgttaatgt ttcaatttct tcagctattt tattgcctat cccccctagt
     7141 cttattggat caggactgtc ttttaaaacc ttgctgacaa caatatctcc atcagcagac
     7201 tttgcacctt cagcgacaac aataatactg aaatgtttgc ccgccttttt cctttcaagg
     7261 actttatcgg caaccttgtt tatattgtaa ggtatttctg gaattaatat gacatcagct
     7321 ccacctgcaa tcccggactc aattgctatc catccggcat aacgccccat aacctccaat
     7381 atcataaccc tgtgatggga ttctgctgta gaatgcagtt tgtctatagc ctcagttgca
     7441 gtttcaacag ccgtcataaa gccgaaggta taatctgtac ctgaaaggtc attatctatt
     7501 gttttaggta cgcctataac tttcaaacct tttctggaaa aatctcttgc actggtaagt
     7561 gtcccatctc cgccgatcat tatcatgcaa tctattccaa gcgaattgat gttgttaatt
     7621 acctgttcag acatatcttt gtattctacc ttgccattta cctcaactct aaacttaaaa
     7681 ggattatctc tgtttgatgt tccaagtatt gtgccgcctt tatcaagtat tcctgaggca
     7741 tctcccgggc caaaaatcat atagtcatta agtaccaaac ccctgtaacc atctttaaat
     7801 ccaattactt cccagccata tttaaacatt gcagttttag ccacagctct taatactgca
     7861 ttcagacctg ggcaatcacc accgcctgta agtactccaa tacgttttgt cattgaatcc
     7921 cctccattat tattttaatg ttacaaaaaa ctatctgttt ttagcattcc aagtgcatct
     7981 ttccaaattc tttcaatcca ttaaacctat tttgccttaa agcctcgtaa aggactattg
     8041 ctacggaatt tgccaagttc aaagacctta ttgtttccct catgggtatc cttacacacc
     8101 aatccttatt agccttaagc aggtcttcag gcaaaccagc cgtttccttg ccaaaaacaa
     8161 tgaaacagtc ttcaggataa ataaaatctg tatatactgc agcagctttg gttgtagaat
     8221 agtaaaaact atttgaagga tattttctcc ttaattcctc gaaggaatca taatagagta
     8281 tttctaattt gttccaatag tccagaccgg ctctcttcag gtacttgtct tccagggaaa
     8341 atcccagagg tcttacaaga tggagggcag taccggtagc tgcacatgtt cttgcaatat
     8401 ttcccgtgtt ttgcggtatc tcaggttcaa caagcactat gttcaaacta atatccttcc
     8461 ttccaattag ttaagtgatt acagcatcaa tgtgttcatt cagtttaata ggtgcacctg
     8521 taatccatct atgcatttag tttaatagat gcacttatgt ttcatgtatg catttagtta
     8581 atagatgcac ttatgttcaa tttctactat ctatattaac attattttat cataacaaac
     8641 aagttgaatg aagtattttt tgcataaacc ttactacatg gtttctttca ccacattaat
     8701 ttaataaaac ttcataacaa tgatatagca ttgttatatc attgttataa taccactctt
     8761 agcttttatt tctctatact cttcattgaa tctctttttt atattgcctc caaaacgaac
     8821 tccatatgct gtggctggta tctcttatga ctggtctatg gtttactacg aggaatggtg
     8881 tgaactgtgg ctgaaaatca aagtattcac catagaggac aaataaggct tgctctgagg
     8941 tatctccgcc ttgcggtttc gttctctata cagcttgctt ctgtaagcat atcccttgct
     9001 gtttttatgg caaactgcat aagttcacct gtttctttac tccagtaaca gccgtcatat
     9061 accaaccagt gttcgaaagc cggacaatat catatgattg aaccaaacct gtcacgaagc
     9121 ctttctgcat taccgctgtc tgttctgtgg tgatacttct tctgtggagg atttggccgg
     9181 taccggctta cactgtgtgc aataatttta atttctgact catcaagcac cagccagccg
     9241 gatttctcgc cagtaggaat gcctatattt gcattgggtg tttttgtcca ccatctaatt
     9301 atctgccggg ctctataggt gagtcattcg ctccctttgt ggctctgtgc cagatttatt
     9361 cactttttac cccgcctttt tctccagttt gtcaagtata gacataaaga aaacccggct
     9421 tgtcttttta ttgagtagtc gggtattcca ctatttatgc ttaatactta tcgagaaact
     9481 cctttggtgt taatatttca gggcggtcaa tatcaactac agcaaaatcc ttgtctcctg
     9541 ttatgagcac gtcaacatct tccaatattg ccgttgcaag aataggataa tcatatacat
//
LOCUS       DAOSDD010000179         9515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2689_length_9515_cov_5.006871, whole genome shotgun sequence.
ACCESSION   DAOSDD010000179 DAOSDD010000000
VERSION     DAOSDD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2689_length_9515_cov_5.006871"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK733_14125"
     CDS             <1..188
                     /locus_tag="PK733_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11716.1"
                     /translation="RINRSNIRTVPIQQHTLHILYLLIDLKTNSDMNPGTIKSRKKMK
                     ELSILITLSILFEILFY"
     gene            515..2260
                     /locus_tag="PK733_14130"
     CDS             515..2260
                     /locus_tag="PK733_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ11717.1"
                     /translation="MSKSKTDIGNLYSVRKIKDLKDMLEQSANLFAAKNAFLVKNEDG
                     NYKGITYSQVKKDVDALGTALLSLGLKDKFIAVLGENRYEWCITYLSTVNGTGVIVPI
                     DKELPIEDIEYVLFKSNASAIVFSGKYTESIKKLSQNNSAIKYYINMDSDNDDEFSLS
                     FKQLVEKGKKLISSGSRDFVNATIDNTKMSVLLFTSGTTGFAKGVILSHKNICSNIMS
                     ICSTVYVDSTDSSLSILPLHHTYECTIGFLCIIYSGGSIAFNEGLRHIGRNLKEVRPT
                     LMISVPLLLENVYKKIWDQAGKKFGGKVSLRIAIWLGAFLKSFLKIDIRRKLFKAIHE
                     NLGGRLRLIITGAAAIDPRVSKGFRNMGIRVLQGYGLTECSPLATGNRDAAFKDNSAG
                     LPIPGVQVRIDNPDEKGIGEIVVKGDNVTVGYFENEKATKNSMKDGWFYTGDLGRMDK
                     SGYLYITGRIKNIIVTKNGKNIYPEELEASINRNPFVQESLVWGKLDESSGDTHVCAQ
                     ILPNLEAIKEKLKLINITKEDISKVLGDVIKSVNKNLPLYKHIKNFSIRDNEFIKTTT
                     KKIKRHLEQKPNENS"
     gene            2395..2583
                     /locus_tag="PK733_14135"
     CDS             2395..2583
                     /locus_tag="PK733_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11718.1"
                     /translation="MNIEKVRLIYFSPTGSTEKILNYILKGLGLDIFNINTTNLTVPV
                     NRKECMINDKVQIDIVDG"
     gene            2597..2830
                     /locus_tag="PK733_14140"
     CDS             2597..2830
                     /locus_tag="PK733_14140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOJ11719.1"
                     /translation="MFKILERTGRRNLKICIKTEMMNYPSRVQIEFGGRVGWIMLDQI
                     WCIDKSRLMKKAGNVSEEDIFQVKGIIKEMLVD"
     gene            2993..3592
                     /gene="yihA"
                     /locus_tag="PK733_14145"
     CDS             2993..3592
                     /gene="yihA"
                     /locus_tag="PK733_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOJ11720.1"
                     /translation="MLINLNNARFEITAVKPYQYPSDNVPEIAFVGRSNVGKSSIINT
                     LLNRKNLARVGATPGKTREINFYNIDDKLHFVDLPGYGYASVSKIKKSTWSNMIETYL
                     NSRKKLELIIMMVDIRHTPSENDRIMYEWLKSREIQHIIVATKADKISRGETGRRIND
                     IQATLGSGENVPVIAFSASTRQGKDEIWKQIYRILDLTC"
     gene            complement(3782..4882)
                     /locus_tag="PK733_14150"
     CDS             complement(3782..4882)
                     /locus_tag="PK733_14150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOJ11721.1"
                     /translation="MKPCLEDVLKRFNIKGEFTEAQPVNSGHINDTYVVCFKQNNNTR
                     QRYVLQRINHYVFKEPDKLMDNIIKVTAYLRKKIESSSGNPNRETLSFIPVSSGKFLH
                     VSEDGSYWRVYSYIEDAITYQVVENSNHFYNAGKAFGRFQMLLSDFPVDDLYETIPDF
                     HNTPKRLKALIETIDKDINNRAINVSSEIDFVMKRAEETSIIVNLLDENKLPLRVTHN
                     DTKFNNVLIDTNTGEGICVIDLDTVMPGTSLYDFGDSIRSGANTADEDEPDLAKVWMD
                     IGLFEQFTRGYLEMAGGFLTPTELEYLPFSAKLITFELGMRFLMDYLNGDIYFKIHRD
                     GHNLDRARVQFKLVSDMEAKYDKMKNIVEKYK"
     gene            5411..8578
                     /locus_tag="PK733_14155"
     CDS             5411..8578
                     /locus_tag="PK733_14155"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOJ11722.1"
                     /translation="MKFESFVSGDENYRPIAFWSLNHRLSKEELSWQIKELAEKGFQG
                     FFMHSRSGSLTPYLSKMWFDAIDEIIIEAKKNGLKAWLYDEDPYPSGAAGGKVLADHP
                     EYRAKVLEIKKQHINGPANISLEIPLSKVIKASAIKTEDNVIVEKINIQDYVGILRKN
                     WKEKRYYHSYYPTTEHEALPQYRSDTSSAMLTLEWDVPCGEWDIVIWYVYEQGKYQLY
                     DSYPDLMNPNAVNYFIETTYEQYKKRFSQYFGNIIPGIFMDEPKFVALPYAWNNLLKD
                     EFEKRKGYSFDIALLSLALDFNDSCRSRKDYWEVVSQLFHEAFAGSISKWCEENGLLL
                     TGHASPEEEPVWQSIMTGNLMDFQKYLHIPGTDIITYMIGDKRHPILNLGPKLVASVA
                     RQWRDGKALCECFGVTEWKLKIADMIWISNWLFSLGINIIVPHTLSYSIDGLRKKDAG
                     PSQFYQNPYWEYYKIYSDYLSRLGYILGESEPVVKTALIFSVRNYMSLERIHDLEAQE
                     LRDRFVYLFNKLLRNHIQFDLVSDTDFEKCTVEGDSIVIGKCSYKHIIISEMICPTRE
                     FVDKVKEYCERGGSLIIEAADKAKHQKDYNVYTKERKYLIDCIRNFNDIIFYKINGDA
                     NKGFSDQGINEVVALVKDDILIEGEYSDDIIVNHHKKDETNIYFLNNTSENEIKASIT
                     CVSNENFITEECIAEEWNLKYINKRVIPKHEDKWKVIIAPKSAAVIILKNNEIKQNNK
                     QNNEEAKCGINLLSQSDNCNNVKTTELYSSWSFKICKRNVLILDKWLLETENNQEYGK
                     INITGRGVIVSPPWTGQNDNLFGKAKTLIYKTNFWLDEQIDDMGIVLEKSAIKGNYEI
                     YVNGILCENYFNSREYDCYNIEYPLKSNTFHSALQKYYRKGLNVVAVKVKLSEVNDGL
                     LEPLRLFGSFKVQLNDGCSIGATLSKIEDEDHISYGSWTYQGYPNFSGSAIYEQEIYI
                     DKNFEEERCFIQVDVGKDIAEVTVNGILAGISAWEPYVIEITDMINNCKNNIKIKVTN
                     TLENLLYGTQKPSGLLGPVKIITHS"
     gene            8717..>9515
                     /locus_tag="PK733_14160"
     CDS             8717..>9515
                     /locus_tag="PK733_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ11723.1"
                     /translation="MTNRERFINILNFKKVEDRLPLVEWAAWWDKTIERWKKEGLPQG
                     LDIEGTLEYFGFDKLICIVAAGIDKKAPKPLIHGGGLIKDEYSYNELRKYLFTDSIIE
                     NIKKHALRLKERHEKGEIIIRLWLDGFFWFPRTIFGIERHLYAFYDQPELMHRINEEL
                     TEFNIRAVEELFKIIKPDMVGIAEDMSYNHGPMLSYSLFKEFLLPYYQQLVPYIKSYG
                     VHVLVDSDGDITSMIPWLKEAGIDGVYPLERQAGVDLCKIREQYPDFL"
BASE COUNT         3473 a         1352 c         1842 g         2848 t
ORIGIN      
        1 ttagaatcaa caggtcaaat atcagaactg tacctataca acagcataca ctgcatattc
       61 tatatctgct tatagattta aaaaccaata gcgatatgaa tccgggaaca attaaaagca
      121 ggaaaaaaat gaaggaactc agcatattga tcactctttc tattttattt gaaatattat
      181 tctattaaca taatatgttt gagaaaaaat tatgtgaaag atttgatgta aaatagagtg
      241 ctgcgacaga aggaatacct tccgcataat tactttataa ttgcataata taatatgcac
      301 ccctgttaca gaaaaattct tgtagattat tagtatttgt gttagtataa aattaataaa
      361 cgattagcat atatatacct ttatacttat actactatgc caatttggaa agtgatgtct
      421 tccgggagga attttgatag caatgggaag atatagcttc ccattggcaa cataacaaag
      481 cattacatat ataattttag aaagcagggg cgttatgagc aaaagcaaga cagatatagg
      541 aaatctctac tctgtaagaa aaattaaaga ccttaaagac atgctggaac aaagtgccaa
      601 tttattcgcc gcaaaaaatg cgttccttgt taagaatgaa gatggtaatt acaaaggtat
      661 tacttactct caggttaaaa aagatgttga tgcccttgga acagcgttat tgagtttggg
      721 actaaaagac aaattcattg ccgttctcgg agaaaaccgt tatgagtggt gtataaccta
      781 cctttctact gttaatggta ccggagttat agtaccaatt gataaggagc ttccaataga
      841 ggatatagaa tatgttctat ttaagagcaa tgcatcagct attgtatttt ccggcaaata
      901 cacagaaagt ataaaaaagt tgtcgcaaaa caattcagct ataaaatatt atattaatat
      961 ggatagcgat aatgatgatg agttttccct atcttttaaa caattagttg aaaaaggcaa
     1021 aaaattaatt agcagcggaa gtagagattt tgtgaatgct acaatagata atacaaaaat
     1081 gagtgtatta ctctttacat caggtactac cgggtttgca aaaggtgtta tactatcgca
     1141 caaaaatata tgttcaaata taatgtctat ttgctccacg gtctatgttg atagtacgga
     1201 ctcctcactc tctatcttac ctttgcatca tacatatgag tgtaccatcg gctttttatg
     1261 tataatatat agcggagggt caatagcttt taacgaggga ttaagacata taggacgcaa
     1321 cttaaaggaa gtaagaccta ctttgatgat ttctgttcct ctgcttcttg aaaatgtgta
     1381 taaaaaaata tgggatcagg ctggcaaaaa attcggcggc aaggtttctc ttcgcatagc
     1441 aatttggctg ggagcatttt taaagagctt tcttaaaatt gatatacgac gcaaactatt
     1501 taaggctatt catgagaatc taggcggcag gctgagattg attataacag gggctgcagc
     1561 tattgacccc cgggtttcaa aaggtttcag gaatatggga atacgggttc tgcaaggcta
     1621 tggcctgaca gagtgttcac ccctggcaac aggaaacagg gacgcagctt ttaaggacaa
     1681 ttcagcagga ttgccaatac caggagttca ggtgcgtatt gataatcctg atgaaaaggg
     1741 aattggagaa attgtagtta aaggtgataa tgttacggtt ggttactttg aaaatgagaa
     1801 agctacaaaa aacagtatga aagatggctg gttttataca ggtgacttgg gaagaatgga
     1861 taagtcaggc tatttatata ttacaggaag aattaaaaat attattgtaa ctaaaaatgg
     1921 aaaaaatatt tatcctgaag aactggaagc aagtatcaac agaaatcctt ttgtacagga
     1981 gtctcttgtg tggggcaagc ttgatgaatc ctcaggggat acccatgttt gtgcgcaaat
     2041 tctcccaaat cttgaggcaa taaaggaaaa actaaaactc attaatataa caaaagaaga
     2101 tatttcaaaa gttttgggtg atgtaattaa aagtgtaaat aaaaatttgc cattatacaa
     2161 gcatattaag aatttctcaa taagggataa tgaatttatt aaaactacga caaaaaagat
     2221 aaaaagacat ctggaacaaa aaccaaatga gaacagttaa aattacaaaa acaatagaat
     2281 taagtgagta gtttggatgg gcagcacgga aaaacaaagc atgctgccct atcctaataa
     2341 tgctgtaaat gacagatatt aggatgtcaa aaaaatttat tattggagta atttgtgaat
     2401 atcgaaaaag taagattgat ttatttttca ccaacaggta gcactgaaaa aattctcaat
     2461 tatatattaa aaggtctggg tctggacatt tttaacataa acactacaaa tttgacagtg
     2521 ccggtcaacc gtaaagaatg tatgattaat gataaagtac agatagatat agttgatgga
     2581 taaataatta ttgcacatgt tcaaaatcct cgaaaggact gggcgcagaa atttaaagat
     2641 atgcataaaa acagagatga tgaactaccc atcaagagta caaattgaat tcggtggaag
     2701 ggttggctgg attatgttag atcagatatg gtgtatagat aaatcaaggt taatgaaaaa
     2761 agccggaaac gtttcagaag aggatatttt tcaagtaaaa ggcatcatta aagaaatgct
     2821 tgttgattaa ttgaggcttt gctaatataa agtttgaaga atacaaaatg taatttgtat
     2881 aggaaatatg aaaacaaaaa gaaaggagat gccacgaagt ctgtaaaaaa tatagataag
     2941 caaacatttg aggtccaggt atacatttgt ctatattttc agtggcagga caatgttaat
     3001 taatttaaat aatgcaagat ttgaaattac tgctgtgaaa ccataccagt atccttcgga
     3061 taatgttcct gaaatagctt tcgttggaag atccaatgtt gggaagtcat ccattatcaa
     3121 tacattattg aatagaaaaa acctggcaag ggtaggtgca acaccgggga aaacaagaga
     3181 gataaacttc tataatattg atgataaact tcattttgtt gacctcccgg gttatgggta
     3241 tgcaagtgta tcaaagataa agaagtcaac atggagtaac atgatagaaa cttatttgaa
     3301 ttcacgcaag aagcttgagt taatcataat gatggttgat atacggcaca ctccttcaga
     3361 gaatgacaga attatgtatg aatggttaaa aagccgtgag atacaacata taattgttgc
     3421 tactaaagct gataagattt cccgagggga aacaggcaga aggataaatg atatacaggc
     3481 aactcttgga agcggcgaaa atgtaccggt aattgcattt tcagccagta ccaggcaggg
     3541 aaaagatgaa atatggaaac agatttacag gatattggat cttacatgct aagctgatct
     3601 gaactgcgcg taaattaaac atggaacaga gaatggaaaa tagtcggcat aaaatatagg
     3661 tcagaaaact gaaaacagaa aacagagaac agaaaatgga agatagaagg tgggatacct
     3721 ttttctgttc tccacttatc cccaactttt gttatccagg gagtatgtac tctgatctta
     3781 attatttata tttctctact atatttttca ttttatcata cttggcttcc atatctgaca
     3841 ctaatttaaa ctgcactcga gccctatcca ggttatgacc atctctgtga atcttgaagt
     3901 aaatatcacc attcagataa tccataagaa acctcattcc aagttcaaag gttataagct
     3961 tggcggaaaa aggcagatat tcaagctcag ttggcgtaag aaagcctccg gccatttcca
     4021 ggtaaccacg ggtaaactgt tcaaatagac caatatccat ccatactttt gctaaatccg
     4081 gttcgtcttc gtcagcagta tttgcccctg aacggataga gtctccaaag tcatataatg
     4141 atgtaccggg cataaccgta tccaaatcaa taacacagat accttcgcca gtattagtat
     4201 caatcaaaac attgttaaat tttgtatcat tatgggttac acgtaaaggt agcttatttt
     4261 catccagaag attaacgata attgaagttt cctcagctct tttcatcaca aaatcaattt
     4321 cagaactgac atttattgca cgattgttaa tatccttatc aatagtttca atcaaggctt
     4381 tcagtctctt cggtgtatta tgaaaatcag gtatggtttc atataaatca tcaactggaa
     4441 aatcgctaag tagcatctga aatctgccaa aagcttttcc ggcgttataa aaatggtttg
     4501 aattttcaac tacctggtat gttattgcgt cttctatata tgaatagaca cgccagtagg
     4561 agccatcttc acttacatgc aaaaatttac ctgacgatac aggaataaag cttagggttt
     4621 ccctatttgg attgccgctg ctggattcaa tttttttgcg taagtatgca gtaaccttaa
     4681 ttatattatc cataagttta tcaggctctt tgaaaacata atgatttatt ctttgaagaa
     4741 cataacgttg cctggtattg ttattctgct taaaacagac aacatatgta tcattaatat
     4801 ggccgctgtt aacaggttga gcttcagtaa actccccctt aatattaaaa cgcttaagaa
     4861 catcctctaa acagggtttc ataaaacata acactcccat atcatctaat ataaatattt
     4921 gacatagtat actttaagta tatttttata tttctaactt gtgcatgact acttattgta
     4981 aataccatag aaatagatta acttcattgc ttgattttgt caacctgttt ttaataggcg
     5041 tagtaatata tcaaaatgta aatgatgtag tacaaataat cgtcaataat aaattttgaa
     5101 tataaatatt tatttttgat gatgtttgga acattgaaat ttaagacata cataattata
     5161 ataataatta aatataaact ttttataaag gttaataaac aattatatca gtggcttaca
     5221 aatataaagt agattattta gaagaaaaca tgtgaaggag tatagaggaa attataaaaa
     5281 ttctaaattg ctttattctg aagtttacac tattttcacg tacaatgaat gccactgcat
     5341 ggggatttct tacaggtcat tagtatataa atacttttga cattatttcg taatatgagg
     5401 ggtgttatgt atgaagtttg agtcgtttgt ctccggagat gaaaattata gaccaatagc
     5461 gttttggtct ttgaaccata gactttctaa agaagaacta tcctggcaga ttaaagagct
     5521 tgctgaaaag ggctttcaag gtttctttat gcattcaaga agtggatcgc tcactcctta
     5581 tctttcaaaa atgtggtttg atgctattga cgaaattatt atagaagcta aaaaaaatgg
     5641 gttaaaagcc tggttatatg atgaagaccc atatccaagt ggtgccgcag gaggaaaggt
     5701 cctggccgat cacccggaat atagagcgaa agttttggaa ataaagaaac aacatattaa
     5761 tggtcctgct aatatttcat tagaaatacc acttagtaaa gtaattaagg cttcagctat
     5821 taagacagaa gataatgtaa ttgtagaaaa gattaatata caggattacg ttgggatatt
     5881 aaggaagaat tggaaagaaa aaagatacta ccattcctat tatcctacta ctgaacatga
     5941 ggcattacca cagtaccgtt cagatactag tagcgcaatg cttacactgg aatgggatgt
     6001 tccctgtggt gagtgggaca ttgttatatg gtatgtttat gaacagggga agtaccaatt
     6061 atatgattca taccctgatc ttatgaatcc aaatgctgta aactatttca ttgagactac
     6121 atatgagcaa tataaaaaga gatttagcca gtatttcggg aatataattc ccgggatatt
     6181 catggatgaa cctaaatttg ttgctttacc gtatgcatgg aataatttat taaaagatga
     6241 gtttgaaaag agaaaaggat atagctttga tattgcgcta ttatcattag cattagattt
     6301 taatgatagt tgcaggagta gaaaggatta ttgggaggtt gtctcacagc tatttcatga
     6361 ggcttttgca ggtagtataa gtaaatggtg tgaagaaaac gggctattat taacagggca
     6421 tgcaagtcct gaagaggagc cggtttggca atcaataatg actgggaact tgatggattt
     6481 ccaaaaatat ttacatatcc ctgggactga tataattact tacatgatag gtgataaaag
     6541 acatcccata ttaaatttag ggcccaaact agtagcctct gtagcacgcc aatggagaga
     6601 tggaaaagct ctgtgcgaat gttttggtgt tactgagtgg aaacttaaaa ttgcagacat
     6661 gatttggatc agtaactggt tattttcatt aggtataaat ataatagtgc ctcatacttt
     6721 aagctattct attgatggcc tgagaaaaaa agatgcaggt ccttctcaat tttaccagaa
     6781 cccatattgg gaatactaca aaatatactc tgactattta tcaaggcttg gatatatcct
     6841 tggtgaaagt gaaccagttg taaaaactgc tcttatattt tctgtaagaa actatatgtc
     6901 tttggaacgg atacatgatt tggaagctca agagctaagg gacaggtttg tatatttgtt
     6961 caataagctt ttacgcaatc atatacaatt tgatcttgta agtgacacag atttcgaaaa
     7021 atgtactgtt gaaggggaca gtatagtaat agggaaatgc agttataagc atataattat
     7081 atcagaaatg atttgcccaa ccagggagtt tgtagataaa gttaaagaat attgtgaacg
     7141 agggggaagt ctgataattg aagctgcaga taaagctaaa caccaaaagg attataatgt
     7201 ttatactaaa gaaagaaaat atctcattga ttgcataaga aattttaatg atattatttt
     7261 ttacaaaata aatggtgatg ctaacaaagg attttctgat caaggaataa atgaggtagt
     7321 tgctcttgta aaagacgaca ttcttataga aggtgaatat tcagatgaca taatagtaaa
     7381 tcatcataaa aaagatgaaa caaatatcta ctttttaaat aatacttcgg aaaatgaaat
     7441 aaaagcttcc atcacatgtg ttagtaatga aaattttata acggaggaat gtatagcgga
     7501 ggaatggaat ttaaaatata taaataaaag agttatacca aaacatgaag acaaatggaa
     7561 agttataatt gctccaaaat cagcagcagt tattattctt aaaaacaacg aaattaaaca
     7621 aaataataaa caaaataatg aggaagcaaa atgtggaatt aatttattat cacaatcaga
     7681 taactgcaat aatgtaaaaa ctacagagtt atattcaagt tggtcattta agatctgcaa
     7741 aagaaatgta ctcattcttg acaaatggtt gctagaaaca gaaaataacc aggagtatgg
     7801 caaaataaat ataactggca ggggggtaat tgtttcacca ccgtggaccg ggcaaaatga
     7861 caatttgttt ggcaaagcaa aaacacttat atataagacg aatttttggc tggatgagca
     7921 aatagatgat atgggcatag tacttgagaa gtcagctata aaaggtaatt atgagatata
     7981 tgttaatgga atactttgtg aaaattattt taatagcaga gaatatgact gctataacat
     8041 tgaatatcct ctgaaaagta atacatttca ttctgcgtta cagaaatatt atagaaaagg
     8101 gttaaatgtt gtggcggtaa aagtaaaatt gagtgaggta aatgatggct tattggaacc
     8161 attacgtcta ttcggcagct ttaaagttca gttaaatgat ggttgcagta ttggtgcaac
     8221 cttaagcaaa atagaagatg aagatcatat ctcatatggt agttggacat atcagggata
     8281 tcctaatttc tcagggtcag caatatatga acaggaaatt tatattgata aaaactttga
     8341 ggaagaaaga tgctttatcc aggtagatgt aggtaaagat attgcagaag tgactgttaa
     8401 tggcattcta gctggaattt cagcatggga accttatgta attgaaatta ctgatatgat
     8461 aaacaattgt aaaaataaca taaaaattaa agtcactaac acacttgaga atttactata
     8521 tggaacgcaa aaaccatctg gtctactagg tccagttaaa attataactc atagttagat
     8581 atagagaagg tattcatatg gcaaccaagc taaaagatta tggaacaatg actctcacaa
     8641 gtaaaaaata tataaatgca ttaagcaaag gtaagatctt gaatataaca cagaaaccaa
     8701 ggaaaggagt ctagtaatga caaacaggga aagatttata aatatattaa attttaagaa
     8761 ggtggaagat aggcttcccc ttgtagaatg ggccgcttgg tgggacaaaa caatagaacg
     8821 gtggaaaaaa gaaggtcttc ctcagggact tgatatagag ggtacgttag agtatttcgg
     8881 ttttgataaa ttgatatgta ttgtagcagc cggtatagat aaaaaagctc caaaaccctt
     8941 aattcatggc ggaggtctga ttaaagatga atattcttat aacgaactga gaaaatatct
     9001 ttttacagac agcattattg aaaacataaa gaagcatgct ttacggctaa aagaaaggca
     9061 tgaaaaaggt gaaattatca tcagactgtg gctggatgga ttcttttggt ttccccgtac
     9121 tatttttggg attgaaaggc atttgtatgc gttttatgat caaccggaat taatgcatag
     9181 aataaatgaa gagcttacgg agttcaatat ccgcgctgtt gaagaattat ttaaaataat
     9241 aaaacctgat atggtaggga ttgcagagga tatgtcatat aatcatggac ctatgttatc
     9301 atatagcctt tttaaggaat tcttactacc ctattatcaa cagttagtac cctatataaa
     9361 aagctatggg gtacatgtgc tggtagatag cgatggtgat ataacaagca tgataccttg
     9421 gcttaaagag gcaggaattg atggggttta tccattagaa cggcaagcag gagtagatct
     9481 ttgcaagata cgtgagcaat atcctgattt tttaa
//
LOCUS       DAOSDD010000180         9515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2690_length_9515_cov_4.734672, whole genome shotgun sequence.
ACCESSION   DAOSDD010000180 DAOSDD010000000
VERSION     DAOSDD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_9515_cov_4.734672"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..336
                     /locus_tag="PK733_14165"
                     /pseudo
     CDS             235..336
                     /locus_tag="PK733_14165"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
     gene            complement(458..2860)
                     /locus_tag="PK733_14170"
     CDS             complement(458..2860)
                     /locus_tag="PK733_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11724.1"
                     /translation="MQLFLSFKKKYNKRSFFTKMLLIYFIFTFVVVLSSSYILTGYLN
                     KTLEKEIYKSNTHLLNQVKIFADTYLLEKVYSIVTEKFLDISKDENISNFYSSNNHDN
                     AGTLYRVYNNIENTKINNNFIDSINIYRKSDDMLVSSREGICFNVSSQESNVKGSINT
                     GLLKQIMSSNEPQKWISPQENYVIGKSKSVISFTQAIPMLSSIDERLGCVIINIDADV
                     FFKSLSNIPSTNNIGDLLIIDSKGNLFAHSNLESFAEGLEYTSFLKGMLAAADDGFKT
                     LKIKEELVGVSWVKSSINDWKYISIVPINMLNRQLFVTKQFAFLVIGVVLIFSFCCLN
                     IITSRLYKPLNILIKSTRDKFGTTRDNLDFQRTNDEITYINGIISNLYKRVEEMENTL
                     YDNRRLIEYGIAMDILNGNIADKDEIKKTLEIANINFKYKYCLIMLTEIKNQQFLKLP
                     LEQRKFVTYKMIELINRGFRNKSACISVCHPSNNIITICNFDDYDTVYNSAAITLKLL
                     ETDFKLSYNIAISEPVLDLLVLNRVYDTTAGYLKYSFIYGYGNIFTHHMINKLEFSDN
                     KSDIYNTGTNKPVVPDNLELLLRSCKVEYFKNEIVQVIETIKNSEYSYSYAQNALLQI
                     INSICRVSYEQNIVCHELDKNRLSIEFNNISSIDECKQWIFNLVDLYSQSVNMRNASI
                     DDEIISKIIHYICDNIDNQISLNTVSDNFNISPSHLSKIFKSGTGMNFSDFVINKKLE
                     QAAKLLLNCRKMSVTEIAETLGYFNPSYFTKLFKEKFGMTPVQLRKKNSVVSIELFGD
                     SS"
     gene            3228..4139
                     /locus_tag="PK733_14175"
     CDS             3228..4139
                     /locus_tag="PK733_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11725.1"
                     /translation="MNKDSSIKKYIKYRALVFMFIPGLIYYIIFHYMPMYGLIISFKD
                     FRILDGMMASPWVGFKHFKTVFGSDDFWVVFKNTLVISGLKLIFNFPAPIIFALLLNE
                     VKNMQFKKTVQTISYMPHFLSWVVLAGIVVNFLSPSTGPINMILKVLGAKPIYFVADR
                     KWFREVLVLSAIWKEVGWGTIIYLAALSNINLELYEAAVLDGANRFKQTIYVTLPSIS
                     PVIIIMFIFAVGAIVNDDFDQIFNLYNPAVYSVGDVLSTYIYRMGLENMQYSFSTAVG
                     LFKNTISFTLIIITNYLSKRYSDYGLW"
     gene            4151..5044
                     /locus_tag="PK733_14180"
     CDS             4151..5044
                     /locus_tag="PK733_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11726.1"
                     /translation="MMGHNTMIQSRGEKVFQVFNYIFLAFVTLITVFPFWEVIRISFS
                     TPAEASRMAFTLWPRETTLDGYMSVLKNPFIWMGYKNTIIRVTVGMTIQMTLMVLVAY
                     PLSKKYFPNRNFWTLVIVFTMFFQGGLIPNYLLIKSLHLDNTLWALVLPRAIDTFAML
                     ILRNYFMSLPVSLEEAAKIDGAGQLTTLIKVILPLSKPILVTVLLWGVVWHWNAWFDC
                     LIYIRDGNKYVLQAILRKIVIDAAPQFTDMTVTIDNVVQPSAEIIKCATIVVSTLPIM
                     MVYPFLQKYFIQGVIVGSLKG"
     gene            5181..6809
                     /locus_tag="PK733_14185"
     CDS             5181..6809
                     /locus_tag="PK733_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11727.1"
                     /translation="MKKFKKVFTLLLAGIMCVILISGCANKDGSKESSKGNGNKAADT
                     SGNSEGAVNNDEVITIDWLPQNDAPVDPDSPIVKEVEKAYNIKFNFIYLERSKETELL
                     NLRISSGDIPDLFRLNDTRFRHYIQQGVLAEIPEDMVKNTAPFMYELVQKNGGKAAWE
                     FPKENGKLYGLPSQSPDGQYHFVPIWRDDWLKNVGIDKVPETLEEAEKAFYKFVKEDP
                     DKNGANDTYALSDKGMSVIFGAFGALPYGNGGGLRWVERDNKVMASAVVPEMKDALIL
                     LNKWYKDGLIDPEFISGENKGQYWGNSVTFWNGKIGFTCPGMFYHINPPYSEKDEGSN
                     NYQNFKKLQGEKATYIEGKPLVGPEGKAGTESWGVFSGNYNVMGKDVANQPKKMEKIL
                     EIANRALSDFDMWTLFRYGIKGETYDIVDGNYTYIGDALTIETRAKMGIAQIGFNANN
                     FGFYNKTQPALYDYADKVAKHFDGYYDVVWGNLESYPQYKSVVEKKITEAYILFISGE
                     RSIDEFDKFVEQLSKAGLEQLTKEANDWYSKFYR"
     gene            7135..>8544
                     /locus_tag="PK733_14190"
     CDS             7135..>8544
                     /locus_tag="PK733_14190"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOJ11728.1"
                     /translation="MNKKWYEELLLIADFMPPGPENYEKYDVEKQVKAIKDMGFNSQH
                     IEAFDISNGDTGIAFFKTEYALEQRRDLFKDYCKQYKELGGNAIVYFNVHWLAENLCE
                     ARPDWYQRNKDGNKLTAGYGSGGTVCVNSPYRDWAFKIIKDLAKYDIKGIFLDGPGFN
                     HKGCFCESCLSEFEKEYGFRYTADNLKNRDVFLKMKEFKRKSIIRFLKEARNTLKEVN
                     PDAVIFINSPQIGPTANGKNNNETIEWQDALLAEGGFFYSDLRSWSIWKPAATAKLLE
                     TQAQGKPAIVAMAGRWGPWSYYLLPPAEMWNTYAQALSNGANIWFGIYDANRDDKRMN
                     TVKEINNFAMSNAKYISGTQSVSKIALVWSFKNADFYQASFDFEDFSGEVNNIDNKTK
                     SDAGKAFNGWYEVLTRSHKLFDVIDDYYLENKDLSKYELIILPNIACMGQKEAANLEQ
                     YVKSGGNIISTFDTGCFDEY"
     assembly_gap    8545..8554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8555..9247
                     /locus_tag="PK733_14195"
     CDS             <8555..9247
                     /locus_tag="PK733_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11729.1"
                     /translation="QDNILKDLFGITSIEGVEELAYDHVEVEKSQFTCDLEQPVIPAP
                     YLHVKVKPAPGVETVMYYREKQASRYCELPAQTEYPFLIHNKLDTMGSGSSVLFTGNI
                     DAAYDMYRIPEYYYMMKNAIELAASSDICVEGDITSLDINIRKKNGSYIIHLINHTSC
                     NGRPISSVIPLKDVKISVRMDKKPENIKALRKNTDLEYTYDKGFATIKIPQIDEYEII
                     ITDTCHDTYHSV"
BASE COUNT         3251 a         1374 c         1785 g         3095 t
ORIGIN      
        1 acaggtggtg tgagaggtcg gaaaataaat taattatttt ccccctactc gatttgattt
       61 ggctataatt attgtaacat aatagatttt gcactatagt taaatataag aattgaacta
      121 tagcagttat atagaaccta aacagttgca taaaattggt aattactaaa taaaatagct
      181 aaatttgaac gggaggagtt aaatggcagc actatttgaa atgccactta taataagatt
      241 agatctgaaa aacaagtggt tatttatcct gattttggac atgaggaact accagatttt
      301 gatgatatga tatatgattt tatgatggga ctgtaaatag tatgagttat tgggagtaac
      361 agtttttaag tgattcaaca ggagttaatg cagtatggat tttaatctaa atcatgtata
      421 tttggggata gttctgattc attgtctgct gaatcactca agaactatct ccaaataact
      481 caatggatac cacagaattt ttctttctta actgtaccgg tgtcatacca aacttttcct
      541 taaataactt ggtaaaatat gaaggattaa aataaccaag agtttctgca atttcagtaa
      601 cgctcatttt cctgcagttt aaaaggagct tggctgcctg ttcgagtttt ttatttataa
      661 caaagtctga aaaattcatg ccggtaccag acttgaatat tttgctcagg tgactggggc
      721 tgatattgaa attatctgag acagtattaa gcgatatttg attatcaata ttatcacaaa
      781 tataatgaat aatttttgaa ataatttcat catcaataga agcattgcgc atatttacac
      841 tttgactgta caaatcaaca agattaaata tccattgctt acactcatct atggatgaga
      901 tattattaaa ctcaatagat aatctatttt tatccaactc atggcaaaca atattttgtt
      961 catagctaac tctgcagatt gaattaataa tttgaagaag agcattttgt gcatagctat
     1021 atgaatattc agagtttttt attgtctcta ttacttgtac aatttcattt ttaaaatatt
     1081 ctactttaca cgatcttaaa agtaattcca ggttatcagg aacaacaggc ttgtttgtac
     1141 cagtgttata gatatcgctt ttattgtcgc tgaactcaag tttgttaatc atatggtgag
     1201 taaaaatatt accatatcca taaataaagc tatatttcaa ataaccagct gtagtatcat
     1261 aaactctatt aagtacaagc aaatctaata caggttcact tattgctata ttatatgaaa
     1321 gcttaaaatc tgtttctaat aacttcaatg taattgctgc actattgtaa acagtatcat
     1381 aatcatcaaa gttgcaaatt gttataatgt tgttagatgg atgacataca ctaatacaag
     1441 cggatttatt tctgaaacct ctattgatca gctctatcat tttataggtt acaaattttc
     1501 gttgttccaa aggtaatttt aagaattgct gatttttaat ttcagtcaac ataataaggc
     1561 aatatttata cttaaagttt atatttgcaa tttcaagtgt tttctttatt tcatctttat
     1621 cagctatatt gccattaagg atatccattg caattccata ctcaattaat ctgcgattat
     1681 cgtaaagtgt attttccatt tcttcaactc ttttatataa attagaaatg atgccattta
     1741 tgtatgttat ttcatcatta gtgcgttgaa aatcaagatt atctcttgta gtgccaaatt
     1801 tatctctggt gcttttaatt aaaatgttca atggcttata caatcgggat gtaataatat
     1861 ttaaacagca aaaactaaaa ataaggacaa ctccaattac aaggaacgca aactgctttg
     1921 taacaaaaag ttgtctgttc agcatattta ttgggactat tgatatatat ttccaatcgt
     1981 ttattgaaga ttttacccag gaaacaccaa ctagctcttc ttttattttc aatgttttaa
     2041 aaccatcatc tgcagcagcc agcatccctt ttaaaaaact ggtatattca agtccttctg
     2101 cgaaactttc cagattacta tgtgcgaaaa gatttccttt cgagtctatt attaataaat
     2161 cacctatatt attggtactt ggtatattgc ttaaggattt gaaaaaaaca tctgcatcaa
     2221 tattaattat tacacaacca agtctttcat ctatgctaga tagcataggg atagcctggg
     2281 tgaaagagat tacagacttg cttttgccga taacgtaatt ttcctgagga cttatccatt
     2341 tttggggttc gtttgatgac atgatttgtt tcagtaaacc agtatttatt gagcctttta
     2401 cgttgctttc ttgtgaagat acattgaaac agataccttc tcgtgaagat acaagcatat
     2461 cgtcagattt tctatatata tttatagaat caataaaatt attatttatc ttggtatttt
     2521 cgatattgtt atataccctg taaagtgtac cagcattgtc atgattgttt gaggaataaa
     2581 agttagaaat attttcatct tttgatatgt caaggaattt ttcagtgaca attgagtaca
     2641 ctttttctaa aaggtatgta tctgcaaata tttttacctg gttaaggaga tgcgtattgg
     2701 atttatatat ttccttttcg agagttttgt ttaaatatcc cgttaaaata tacgaagaag
     2761 ataatactac tacaaaggtg aatataaagt aaattaacaa catttttgta aaaaagcttc
     2821 tcttattata tttcttttta aatgataaaa acagttgcat aagcgtcctc ctggtgttat
     2881 taatttacta tatattatat ataacgcaaa attgatttaa caatagtata caccttatat
     2941 ctcctggcag acaaatatct taatccagtt gttataacaa aataatatat gtaataacaa
     3001 taaaatcata tactttcgca aaaaattata tagaatcaca aattattata tactgaaaca
     3061 cataaataac atctaaatga tcagctaaat gcagttgaac agaatgcaga tattactgtt
     3121 aaaaatgtca gtggaaaatt tgtgtgtagt ttgacaagtt ttttggagtt ctaagaacta
     3181 taaaaaagtg ttaggatttt agacagcgga acaaaggagg caaaataatg aacaaagatt
     3241 ctagcattaa aaaatatatt aagtatagag cattagtttt tatgtttata ccagggctta
     3301 tctattatat aatttttcat tacatgccta tgtatggact aataataagc tttaaggatt
     3361 ttagaattct tgatggtatg atggcaagtc catgggtagg ttttaagcat ttcaaaacag
     3421 tatttggcag tgatgatttt tgggttgttt ttaagaatac ccttgttata agtggactca
     3481 aactcatttt taacttccct gcaccaataa ttttcgcatt actgttaaat gaagtgaaaa
     3541 acatgcaatt taagaagaca gtacaaacaa tctcatatat gccccacttt ttatcatggg
     3601 tagttcttgc aggaattgta gtaaacttcc tttcgccttc aacaggacca attaacatga
     3661 tactgaaggt gcttggtgca aaaccaatat atttcgtagc agataggaaa tggtttcgtg
     3721 aagttttagt actttcagcc atatggaaag aagtaggatg gggtactata atatacctgg
     3781 cagcattatc aaatataaat ttagagctgt atgaggcagc tgtattagat ggagcaaata
     3841 gattcaagca gacaatttat gtaactttgc catctatatc tcctgtaata ataataatgt
     3901 ttatatttgc tgttggagca attgtaaatg atgattttga tcagatattt aacctttata
     3961 acccagctgt atatagtgtt ggggatgtac tttccacata tatttacaga atgggtttgg
     4021 aaaacatgca gtacagtttt tctacagctg ttgggctgtt taaaaacaca atatctttta
     4081 cactgataat aataaccaac tatttgtcta agagatatag cgattatggc ttatggtagg
     4141 gaggagattg atgatgggac ataatactat gatacagtct agaggagaaa aggtttttca
     4201 ggtttttaac tatattttcc ttgctttcgt tacattgatt actgttttcc cgttttggga
     4261 agtaatcaga atatcgttca gcacccctgc tgaggcaagc aggatggcct ttacgctttg
     4321 gccccgggaa acaacgctgg atggctatat gagtgtttta aagaaccctt ttatatggat
     4381 gggttacaaa aatactataa taagagttac agtaggaatg acaattcaga tgacgcttat
     4441 ggtacttgta gcttatccgc tttcaaaaaa atattttcct aacaggaatt tttggaccct
     4501 tgttattgtt tttactatgt tttttcaagg aggattaata ccaaattacc tgctaataaa
     4561 atcattgcat ttggataata cactatgggc attggttctt cccagagcga tagacacctt
     4621 tgcaatgtta atattacgaa attattttat gtctttgcca gtgagtttgg aagaagcagc
     4681 aaaaatagat ggtgcaggac agcttactac tttaataaaa gtcattttgc ctctttccaa
     4741 acctatattg gtaacagtgc ttctatgggg tgtagtctgg cattggaacg catggtttga
     4801 ttgtttaata tacataagag atggaaataa gtatgttttg caggctattc ttagaaaaat
     4861 agttattgat gcagcaccac aatttacaga tatgactgtg actatagata atgtagtaca
     4921 gccttcggca gaaataataa agtgtgcaac catagtagtt tctacacttc ctataatgat
     4981 ggtttatcca tttttacaga agtactttat tcaaggggta atagtaggat ctttaaaagg
     5041 ctgataacat taggcgacag taactaatag cgacagtcgg ttaatgttgt tataataaca
     5101 ggtgtatgat aattgtaatt aatgtacctt atagattgca tgcacctgaa aatcacaaac
     5161 taaaaatgga ggtaagtaag atgaaaaagt ttaaaaaagt atttacattg ctgctcgctg
     5221 gtataatgtg tgtaatcctt attagcgggt gtgcgaacaa agatggaagt aaggaaagca
     5281 gtaaaggcaa tggaaataaa gcagctgaca ccagtgggaa ttcagaaggt gcagtaaata
     5341 atgatgaagt aatcaccatt gattggcttc cgcaaaatga tgcaccagta gatccagact
     5401 ctccaattgt taaggaagtt gagaaagcat ataatattaa atttaacttt atttatcttg
     5461 aacgaagcaa agaaactgaa ctattgaact taagaatatc ttcgggagat attccagatt
     5521 tgttcaggct aaatgacaca cgcttcagac actatatcca gcagggagtt ttagctgaaa
     5581 tccctgaaga tatggtaaag aatactgcac cttttatgta tgaactggtt cagaaaaatg
     5641 gcggaaaagc cgcatgggag tttccaaagg agaatggcaa actttatggg cttccatcac
     5701 aaagcccgga tgggcagtat cattttgtac ccatatggcg ggatgactgg cttaagaatg
     5761 taggtataga taaggtgccg gaaactctgg aagaagcaga aaaggctttc tataaattcg
     5821 tgaaagaaga tccagataaa aatggggcaa atgatacata tgcattatca gataaaggta
     5881 tgagcgtaat tttcggagct tttggagctt taccatatgg taatggcgga ggtcttaggt
     5941 gggtggaaag agataataaa gtgatggctt ctgcagttgt cccggagatg aaagatgctc
     6001 ttatacttct caataaatgg tataaagatg gtttaataga ccctgaattt ataagcggcg
     6061 aaaataaagg ccagtattgg ggtaattctg ttactttctg gaatggtaaa atcggattta
     6121 catgtccggg tatgttttac catattaatc ctccttacag tgaaaaagat gaaggttcaa
     6181 ataattatca gaattttaaa aagctccagg gtgaaaaggc aacttacata gaaggcaagc
     6241 ctttagttgg acctgaagga aaagcaggaa ctgagagctg gggcgtattt tccggcaatt
     6301 ataatgtaat gggtaaagat gtagctaatc agccaaagaa gatggaaaag attcttgaaa
     6361 tagctaatag agcacttagt gattttgata tgtggactct tttcaggtat ggaataaaag
     6421 gagaaaccta tgatatagtt gatgggaact acacttatat aggggatgcc cttactattg
     6481 aaacaagagc gaaaatgggt atagcccaaa ttggatttaa tgcaaataat tttgggttct
     6541 ataataaaac gcaacctgct ctttatgatt atgcagataa ggtagctaaa cattttgatg
     6601 gttactatga tgttgtatgg ggcaaccttg aatcctatcc tcaatataag tcggtagttg
     6661 aaaagaagat aactgaggcc tacattctat tcatatctgg agaaagaagt atagatgagt
     6721 ttgataaatt tgtagaacaa ttaagcaaag ccggccttga gcagttaact aaagaggcaa
     6781 atgattggta cagcaagttc tacagataac gcacaaaaga tactacagta taaaattttg
     6841 tgcgcttatg ttaccttata taatacgcaa aaatctaatt tgttccttgt aaaatcattt
     6901 ttgcgctata taaataaaat tacttaagag ttccagaaat gcgctgtcca ttggcaacat
     6961 aaaaaagtaa tattaatttg gttttagaat tgatcttaat tctatgcctg ataatctgga
     7021 taatgaataa ggtatttgta aaaatacctt attcatatat ataatagaat taatgtataa
     7081 ggatttgata aaaaatgtca gtaattttct tattgcggaa gggagatttt tacagtgaat
     7141 aaaaaatggt atgaggagtt gcttcttatt gcagatttta tgcctccggg tcccgaaaac
     7201 tatgaaaagt atgacgtgga gaaacaggtt aaggcaataa aggatatggg gtttaactct
     7261 caacatattg aagcattcga tataagtaat ggagatacag ggatagcttt tttcaaaacg
     7321 gaatatgcac ttgaacagag aagggatttg tttaaggact actgtaagca atataaggaa
     7381 ctgggtggaa atgctattgt ctatttcaat gttcactggc tggctgaaaa cctgtgtgag
     7441 gcaagaccgg attggtatca aaggaataaa gatggtaata agcttactgc aggttatgga
     7501 agcgggggaa cagtatgtgt taattcacct tatcgtgatt gggcttttaa aataattaag
     7561 gatttggcta aatacgatat taaaggtatt tttctcgatg gaccgggatt taatcataaa
     7621 ggttgtttct gtgagtcctg cttatcggaa tttgaaaaag agtatggttt cagatataca
     7681 gctgacaact taaaaaaccg ggatgtattt ttaaagatga aggaattcaa acgaaaaagt
     7741 ataattaggt ttcttaagga agcaagaaat actttaaaag aagttaatcc tgatgcagtt
     7801 atttttatta acagtcctca gataggccca actgccaatg gaaagaataa taatgaaacc
     7861 attgaatggc aggacgcctt gcttgcagaa ggcggttttt tctactcaga ccttcgttcc
     7921 tggtcgattt ggaaacctgc agctactgca aagctgcttg aaacacaggc tcaagggaaa
     7981 cctgcaattg ttgctatggc aggaagatgg ggcccctggt cttactattt actgccccct
     8041 gcggaaatgt ggaatactta tgcacaagct ctttctaacg gtgctaatat atggttcggc
     8101 atatatgatg caaacaggga tgataaaagg atgaatactg taaaggaaat caacaacttc
     8161 gcaatgtcca atgccaagta tatttcaggt acacaatctg tttccaaaat tgctcttgtt
     8221 tggtctttta aaaacgctga tttttaccag gcatcttttg atttcgaaga tttcagcggt
     8281 gaagtaaata atattgacaa caaaaccaaa agtgatgcag gaaaagcttt taatggatgg
     8341 tatgaagttt taacccgctc acataaactg tttgatgtta ttgatgacta ttatcttgag
     8401 aataaagatt tgtcaaaata tgaactaata attttaccta acatagcgtg tatggggcaa
     8461 aaggaagctg cgaatctgga gcagtatgta aaaagcggag gaaatattat atcaactttt
     8521 gatacgggct gcttcgatga gtatnnnnnn nnnncaggat aatatattaa aagatctgtt
     8581 tgggataaca tctattgaag gtgtagaaga actggcatat gaccatgtag aagtagaaaa
     8641 aagccagttt acatgtgacc tggaacagcc ggttatacct gctccttatc ttcatgtaaa
     8701 ggtaaaacct gctcctgggg tagaaacagt tatgtactat agagaaaaac aggcttctcg
     8761 ttattgcgaa ctacccgccc aaacagagta cccattttta atacacaaca agctggatac
     8821 aatgggaagc ggcagttcgg tattgtttac cggcaatata gatgctgctt atgatatgta
     8881 ccgtattcct gaatactatt atatgatgaa aaatgcaatt gaactggcag cttcaagtga
     8941 tatctgtgta gaaggtgata ttacatcact ggatattaat ataagaaaga aaaacggaag
     9001 ttacattatt caccttatta atcatacttc atgtaatgga aggcctatta gcagcgtaat
     9061 tccgttaaag gatgttaaaa tatctgtacg gatggataaa aagcctgaaa atataaaagc
     9121 cctaagaaaa aatacggatt tagagtatac ttatgataaa ggctttgcta caataaaaat
     9181 acctcaaata gatgagtatg aaattataat aactgataca tgtcatgata cataccacag
     9241 tgtatagttg taatcaaata agcatgtatt gtgataaata aagggacaat atgtatacag
     9301 agccacgtaa tgcttgcgtt acatggctct gcgtatttat ataaatatgg aggatttatt
     9361 aagacatcag aactaaatga cttgggattt tccattttca catattccta tattgaccgg
     9421 gtgtaatacc ttcaaaacgt ttaaaacccg gataaatgta taatattatg tagcttaata
     9481 caataaagta aggtatacta tattagtaag atatt
//
LOCUS       DAOSDD010000181         9478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2700_length_9478_cov_4.606070, whole genome shotgun sequence.
ACCESSION   DAOSDD010000181 DAOSDD010000000
VERSION     DAOSDD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2700_length_9478_cov_4.606070"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..2304
                     /locus_tag="PK733_14200"
     CDS             304..2304
                     /locus_tag="PK733_14200"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ11730.1"
                     /translation="MDILLSKKTKIEKVEKSKLRIFVISLAVIVLILFIVALQMSYTV
                     LKFDKIYKGVFVDRINAGGLSTEELSQKLEAKYKNEYNNVKITISTDKYKEKYLLKDF
                     DFSYNIEDTVKKAFSIGRKGSILKRLYQIFHTNFSSEKIPADISYNTDKVYAVVESFY
                     DNTYIPIDEPCVKLEGSTAVISSGHHGESINKNELYDKINECLENRKTGNIKVPVIIT
                     QQSEINADLLYTSINTDPIDAATKIENGQLKVIPHKNGISVDKKLLSEAVEELKKSEN
                     KVKYVPVSLITPDITEDKLNSNIFRDVLATYKTYFSTDSQLNKDRTENLRLAVEKING
                     KLLTPGDVFSFNETVGERTEEKGYKTAQIFVGGKIVDGLGGGICQVSSTLYNAALKAN
                     LSIVERSNHVFTVSYVQHGLDATVSYGSIDFKFKNSTRWPIKIEGYFTSANELVFVLK
                     GTNENPGLTVELSPQIIKVNEFATIYSDDPNLPEGKTVVKQNGLNGYVVDTYKIVKQD
                     GKEISRTKLNTSVYTSLNKLVSRGTKKVDANQNSQEKLSNSPDKANNDNATKTDSTID
                     SNDATDATDTTDTTDTTDTTDATDATDTTDATDATDATDATDATDATDATGSASNTND
                     STDTTGTAGSADTADTTDTTDTTDTTDTADTADTADTADNTN"
     gene            complement(2400..2891)
                     /locus_tag="PK733_14205"
     CDS             complement(2400..2891)
                     /locus_tag="PK733_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11731.1"
                     /translation="MAKTVAAIFDNRDYAEKAARQIKEQGLRTEDISIIAKEGNADQG
                     YRGNFTGSRDINDNISDGVMTGGVLGGLAGLLIGAGSMAIPGLGIIAAAGPITGLISG
                     AVTGGVVGGLVDLGIPESRGRQYETDIKAGKILFTMKTDEEKIDQVASILRNNGAVSV
                     DTY"
     gene            3338..3913
                     /locus_tag="PK733_14210"
     CDS             3338..3913
                     /locus_tag="PK733_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ11732.1"
                     /translation="MKKALFTKIIFITLIAIFFTLSTYASGDIINESSDALVNLEILL
                     GDGKGNLNLNNNVTRCEFVTLVNRMMGYDEDELPKNLTIPFKDIDQKHWAYKNVKIAL
                     NYGLIKGYLDNTLKPDNYVSRVEAEAILVRALGYENKVVEKWPKGIIDKSSELGINKN
                     LDINQDKLLTRGEACVLIYNALSVTLISSNN"
     gene            4019..4525
                     /locus_tag="PK733_14215"
     CDS             4019..4525
                     /locus_tag="PK733_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11733.1"
                     /translation="MQLQGFLLMIFIFVILPVFVLFSNHTVFFIVMSVILFINSLRLI
                     YYSISGLKHVIPEIDEEDKEFIDNIEASTGFNFKRFDTGADISKYLIIILFYIYCSFF
                     IRSVYIKILISSVIVYWIYHIIKSFKESRHSNIKTSISSILERILNFVIGSSSAFIIA
                     FAAYTILR"
     gene            complement(5708..6037)
                     /locus_tag="PK733_14220"
     CDS             complement(5708..6037)
                     /locus_tag="PK733_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11734.1"
                     /translation="MEDKTFELLTKMYSEFSVKFDKVDSRLDMIDSRLNKLEKGQKKL
                     ETTLENDIATKLQALHEREAINSEKLDEHSKQLNDINNKLNYLALSVNIQDTRLEVIE
                     TSKKKKA"
     gene            6330..7979
                     /locus_tag="PK733_14225"
     CDS             6330..7979
                     /locus_tag="PK733_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOJ11735.1"
                     /translation="MKYIKGQSGNQMTFVTEYLEDYISEENPVRVIDAFVDSLDLDLA
                     GFIRTVPKETGRPPYDPRDLLKLYVYGYFNKIRSSRKLMTECTRNIELFYLLGKLTPD
                     FRTISDFRKDNAKALKKVFSTFVKLCLKLNLYQKELLAVDGSKFRAVNSKNNVYNAEV
                     LEKKLKRIDEHIAEYLSQMDKNDEVEPDAPSPEKIKAAIEELTLRKEKYQGYLEELLK
                     SGETQLLTTDPEARRMHSKDGFHCCYNVQTAVDKGSHLIAEFEVTNHNTDQGLLKDVA
                     EDVKKALEVETVEVVADKGYESREDILNCVMNGIVPNVALKYDKGERLYTIDYEEADI
                     TEDIKNSTKPQDIQKCIKAGVLPSCYENTAIEVELQEQSEVSCFLRNEDGTVTCPIGN
                     ILSKTKMRGKNTIYANKDACRQCPNRCTGSKSYKTVSFGPETIFVPVKMYGSIRCKLN
                     PIPENIPVNPFNHTFDRKDYAPAKKVVLRIKEDIHKIKERMCLSEHPFGTVKWYHGAN
                     YLLCKGKEKATAELGLSFLAYNLRRAINMVGVRRLIEAMQG"
     gene            complement(8087..8178)
                     /locus_tag="PK733_14230"
     tRNA            complement(8087..8178)
                     /locus_tag="PK733_14230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8141..8143),aa:Ser,seq:gct)
     gene            complement(8240..8330)
                     /locus_tag="PK733_14235"
     tRNA            complement(8240..8330)
                     /locus_tag="PK733_14235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8293..8295),aa:Ser,seq:tga)
     gene            complement(8744..9262)
                     /locus_tag="PK733_14240"
     CDS             complement(8744..9262)
                     /locus_tag="PK733_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HOJ11736.1"
                     /translation="MNDLNLSENMLYIIIMGLILIIFITILVMSISNSIKINKLYKKY
                     NKFMACDDGKDLEELLEKCILRTDEVSFKHKEIENHLNELDRNISKCLQKSGIIRFNA
                     FDDVGSDLSYSLALLDGNDNGVVITSLFSRDSSTTYGKPITGGKSKYALSAEEIKAIN
                     DAIRKNRESQYV"
BASE COUNT         3243 a         1569 c         1624 g         3042 t
ORIGIN      
        1 tttattgaat ttaaatattg aaggaacaaa aaaaatctgg tatcgtcatt ataatatgtt
       61 ataataaatg caatcaatga aataaaacct ggcggtttta tctttatgca tttattgaaa
      121 cggtgtgaag caatccgatt agcggattgt aattatgtta tatagatacg atcttagtat
      181 atgtatttaa ttatcagttt atggaacata tgctaataat acgtgtatta gcatatgtta
      241 ttactgtgca ggaatattga tgtttataat aaattaaaca ttgtataggg gtgtttggag
      301 taaatggata tattattatc taagaaaact aaaatagaaa aagttgaaaa atcaaagctc
      361 agaatttttg ttatttctct ggctgttata gtattgattt tatttattgt tgctttacag
      421 atgtcatata cagttttgaa gttcgataaa atctacaaag gtgtctttgt agacagaata
      481 aacgcaggtg gactatccac agaggagcta agccaaaaac tggaagctaa atataaaaat
      541 gaatacaata atgtgaaaat aactattagt acagacaagt ataaggaaaa atatttgcta
      601 aaagattttg atttttcata taatatagaa gatactgtta aaaaagcatt ttccatagga
      661 aggaaagggt ccattttaaa aagattgtat caaatatttc atactaactt tagtagtgag
      721 aaaatcccgg ctgatatatc ctataatact gataaagttt acgctgttgt tgaatctttt
      781 tatgacaata cctatattcc tatagatgaa ccgtgtgtaa aattagaagg aagcacagca
      841 gtaattagtt ccgggcatca tggggaaagt atcaataaga atgaactata tgataaaatt
      901 aatgaatgcc tcgagaaccg caaaactgga aatataaaag taccagtaat tattacacaa
      961 cagtccgaga ttaatgcaga ccttctctat accagcatca atactgaccc gatagatgct
     1021 gccaccaaaa tagaaaatgg tcagttgaaa gttatacctc ataagaatgg tataagcgtt
     1081 gataagaaat tactgtcgga agcagttgaa gagttaaaaa aaagtgaaaa taaagttaaa
     1141 tatgtccctg tttctttaat tactcctgat ataactgaag acaaacttaa ttcaaacatt
     1201 ttcagagatg tactggctac atataaaacc tatttttcaa ctgatagcca gcttaataag
     1261 gataggacag aaaatttaag gctagctgta gaaaaaataa atggaaaatt acttacacct
     1321 ggagatgtgt tttcttttaa tgaaacagtt ggtgagcgca ctgaagaaaa agggtacaaa
     1381 accgctcaaa tatttgttgg cggtaaaatt gttgatggtc ttggaggagg tatttgtcag
     1441 gtaagttcta ctttatataa tgctgcatta aaagctaatt taagtattgt tgaaagatca
     1501 aatcacgttt tcaccgtatc ttatgtccaa catgggttgg atgcaactgt ttcatatggc
     1561 tcaatagatt ttaagtttaa aaattctact cggtggccta ttaaaattga gggttatttt
     1621 acaagtgcaa atgagttagt atttgtgttg aaaggaacaa atgaaaatcc cggattaaca
     1681 gttgagcttt ccccacaaat tataaaagta aatgaatttg caactatata ttctgatgat
     1741 cctaatttac cagaaggaaa aactgtagta aaacaaaatg ggttaaacgg ttatgttgtt
     1801 gatacctata aaattgttaa gcaagacgga aaggaaataa gccggacaaa attaaatact
     1861 agtgtatata cttctttgaa taaacttgtt tcacgcggaa caaaaaaagt tgacgcaaac
     1921 caaaattcac aagaaaagtt gtctaacagt ccagataaag caaataatga taatgctact
     1981 aaaaccgata gcactatcga ttctaacgat gctactgatg ctactgacac tactgacact
     2041 actgacacta ctgacactac tgacgctact gatgctactg acactactga tgctactgat
     2101 gctactgatg ctactgatgc tactgatgct actgatgcta ctgatgctac cggcagtgcc
     2161 agtaatacta atgattctac cgatactact ggcactgccg gttctgctga cactgctgac
     2221 actactgaca ctactgacac tactgacact actgatactg ctgatactgc tgatactgct
     2281 gatactgctg ataatactaa ctaaatttag ggtgcatttt atatatatga agaaaatatt
     2341 ggtaatgagc attatttgat taatgcaccc agattaatag gcttatttgc atgtcatgat
     2401 taatatgtgt ctacacttac agctccgtta tttcttaaaa tagatgcaac ttgatctatt
     2461 ttttcttcat cagttttcat tgtaaataat atctttcctg cttttatatc agtttcatac
     2521 tgccttcctc tgctttccgg aatacctaaa tccacaagtc cacctaccac tccacctgta
     2581 acagcacccg agatcagtcc tgtaataggt cctgcagcag caattatacc aagccctgga
     2641 attgccatac tgccggctcc aataagcaat cccgccaaac cgcccaatac accacctgtc
     2701 ataactccat ctgatatatt atcatttata tcacggctac ctgtaaaatt tcctctatat
     2761 ccctgatcag catttccttc ttttgcaata atagatatgt cctctgttct taatccctgc
     2821 tcctttattt gccttgcagc cttctctgca taatcccgat tatcaaaaat tgcagcaaca
     2881 gtctttgcca tcttcatatt acctccttgt taaaatattg aatatttggt ttttatttaa
     2941 tactactttg ttatgatgct aatggaaaca tatttctgga agataatttt tgctaaccca
     3001 taaggaatat atctgagaga agggtcacaa gaccttctat agatacattt ctctgtgcga
     3061 catcttcccg atgctattaa aaattatccc gaaagacttc gctttccaaa gtgacatagt
     3121 agtattaatt tacacattta gtatgcttta aaatttgtaa aatattctta ataacagcag
     3181 atagcttttt gtagtataat gtaaattaaa tatatgcata tgtgaaatat tattctattc
     3241 acactatgca ttataaaaat agtggacaga aaggagaatg ggcatttcct ctcatgacga
     3301 aagtcacgag tctccacgcc cttaaattat aaattaaatg aaaaaagctt tatttactaa
     3361 aataatattt ataacattga tagcaatatt ttttactttg agtacatatg cttcaggtga
     3421 tattataaat gaatcatctg atgctctcgt taatttagaa atattgcttg gagacggaaa
     3481 agggaattta aacctcaata ataatgtgac aaggtgcgaa tttgttactt tagtaaatag
     3541 gatgatggga tatgatgaag acgaactacc gaaaaatcta actattcctt ttaaagatat
     3601 agatcaaaaa cattgggcat ataaaaatgt taaaatagca ttgaattacg gattgattaa
     3661 aggatattta gataacactt taaaacctga taattatgtc tctcgtgttg aagctgaagc
     3721 tattttagta cgcgctttgg ggtatgagaa taaagttgta gaaaaatggc caaaaggtat
     3781 tatagataaa tccagtgagc tgggtattaa taaaaattta gatataaatc aggataaact
     3841 cctgaccagg ggagaagcat gtgtattaat ctataatgca ttaagtgtaa ctttaataag
     3901 cagtaataat tagtgttttt tactttactc gctttactaa tattggttga gtattctgat
     3961 acatatttaa atacatattg tatcagacat ttataaatat tttttttgag ggatagttat
     4021 gcagttacaa ggttttttat taatgatttt tatttttgtt attttacctg tttttgtttt
     4081 attttccaac catactgtat tttttattgt tatgtcggtt attttattta taaattctct
     4141 aagactcatt tattattcaa tttcaggatt aaagcatgtt attcctgaaa ttgatgaaga
     4201 agataaagaa tttatagata atatagaggc cagtacaggt tttaatttta agcgctttga
     4261 tactggtgct gatatttcaa agtatttaat tataattctt ttttatattt attgttcttt
     4321 ttttattaga agcgtttata ttaaaatatt gatttcatca gttattgttt attggatata
     4381 tcatataatt aaatctttta aagaaagtag acattcaaat ataaaaacca gcatttcatc
     4441 aattcttgaa agaatattaa attttgtaat aggatcatca tctgcattta taattgcatt
     4501 tgctgcttat actatactaa gataattttt cttgcatatt cctttaccat atattacata
     4561 ggatatattt ttgatttccg gtaatattaa ttaatgcaaa agggttctca gtagttgagc
     4621 caagattatt acaaggtcta aatgaggctt tgtaatagtc ggccaaaggt taatatcaga
     4681 tcttgtctta ggagacttta gggtttgata ttagccggag ggaagagtat tatatgtctc
     4741 gtatagctat attgcagtaa aagatactgc aatgtggtta aaagggatgt ataatattcg
     4801 agctaagact accctgggtt ccgcaggcag tttaatatat atacattagc ctgattatat
     4861 tatgtaacag tccctgtgta ttattcaaag ctgctggaga tttgttatag ccgtaaggtt
     4921 atagcaagtt tctcaggtac tcttggtagt cgagcatagg ttagtatagg ttttgtaatg
     4981 ttcataatca gccgaaaggt tgattatgga tatgtagaga tctatattag ctgtagcgaa
     5041 gactactgag ggctcttttg tgttcaaatc tgtactatac tgcctgcgca aaaaagcttg
     5101 acgagaagag aataaagccg aaaatgtcta caatttccgt atcagtgctg gcgaacgcat
     5161 tagtcgagat ggaaccaaca aaaaatttcg atctttcact agacgatgca tatagctgca
     5221 cttcacattc agcaagtgta ctgaggatgc tcaacgtcat attcaccaaa taatgacaaa
     5281 acagtatgcc gtaatgcaag tcttatctac tatgacgtaa caaactatta ttttgaacag
     5341 ataagcagga tgaaacacac aggaatggtt gtggtaaaga aggtcgccgt ggtcctatta
     5401 taagggaatt taaaaggttc cttactaata gtaatgaagc ttttaatgaa gagctcaaat
     5461 acgctccgca gctaatatta tggacaggaa aaggagcatc ccgccattgt aaaattcttg
     5521 atacagacat ggcatggaaa cagtttgaca tacttgagga tacatatttt agagctaaat
     5581 agctccaaca gccgcaaaca caaatccgaa tttttcaaca agctatcaat attcttgcgg
     5641 agcatgaaag gtcactcgcc gaaacaaaag ccattgccca ggttcccatc aaattgatga
     5701 gtacctatta tgcctttttc tttttggacg tttctattac ctcaagtctt gtatcttgga
     5761 tgttaaccga tagtgcaaga taattaagtt tattatttat atcgttcaat tgcttgctgt
     5821 gttcatccag cttttccgaa ttgatagcct cacgctcatg taaagcttgc agtttagttg
     5881 ctatatcatt ctcaagggta gtttcaagct tcttttggcc tttctctaat ttattaagcc
     5941 ttgaatcaat catatctagc cttgagtcaa ccttatcaaa ttttactgaa aattcgctgt
     6001 acatcttcgt taataattca aatgttttat cttccaccag taatagcctc ctgttgcttc
     6061 atgggtaaat catatcacac aatagaaaaa tattgaaggg ttaatttaag ttatttgcag
     6121 taccgagtat tataccaacc tgtagtactt tgcctacagg ttaggaagtg gttcttatga
     6181 gtgaatgaaa atagcaccca tcgtttttct tgaaaagtgc tattttggcc agactgtccg
     6241 aaaagtctac aaaaccagca tttcgtctgg ttatgtttac ataaatatga tataatgtat
     6301 atataataca aagtttcaga ggtgctctta tgaaatatat taaaggacaa tcaggcaatc
     6361 aaatgacttt tgtaacagaa tacctggagg actacatatc agaagaaaac cccgttcgtg
     6421 taattgatgc ttttgttgac agcctggacc tagatcttgc aggctttatc cgcaccgtac
     6481 caaaggagac gggacgtcct ccctatgatc caagagacct actgaagctg tatgtatacg
     6541 gttactttaa taaaatacgc tcaagcagga aacttatgac agagtgcaca agaaacattg
     6601 agctattcta tctgctgggc aaactgacac ctgatttcag aaccatatcg gatttcagaa
     6661 aagacaacgc aaaagcacta aagaaggttt ttagcacctt tgtaaagctg tgtctgaagc
     6721 ttaaccttta tcagaaggag cttctggctg tagatgggtc aaaattcaga gctgtcaaca
     6781 gcaagaacaa tgtatataat gcggaagttc tggaaaaaaa gctaaaaagg atagacgaac
     6841 atattgcgga atatctatcc caaatggaca aaaatgacga agtcgaacct gatgcacctt
     6901 ctcctgagaa aatcaaagcc gctatagaag aattgacact gaggaaggaa aaatatcagg
     6961 ggtatttaga agaactactg aaaagcggag aaacgcagtt gctgacaaca gatccagaag
     7021 ccaggcgtat gcatagcaag gacggattcc actgctgcta caatgttcag acagcagtag
     7081 ataaaggcag tcatcttatc gctgaatttg aagtgaccaa tcataatacc gaccagggac
     7141 ttctcaagga tgttgcagag gatgtgaaga aggcccttga ggttgaaact gttgaggttg
     7201 tggcggataa aggctatgaa agcagggagg atattttaaa ctgtgtaatg aacggaatcg
     7261 ttcccaatgt agcactgaag tatgacaaag gcgagaggct ttatacaata gattatgagg
     7321 aagcagatat aacagaagat ataaagaatt ccaccaaacc acaagacata caaaaatgca
     7381 tcaaggcagg agtactgccg tcatgctatg aaaataccgc tatagaggtt gagttgcagg
     7441 agcagtccga agtcagctgc tttttacgca atgaagacgg aacagtaacc tgccccattg
     7501 gtaatatcct gtcaaagaca aaaatgagag ggaaaaacac catctatgcc aataaagatg
     7561 cctgcaggca gtgccctaat agatgtacag gctcaaagtc gtataaaacg gtaagtttcg
     7621 gaccggaaac tatattcgta cctgtcaaga tgtatggaag tatcagatgc aaactaaatc
     7681 ctataccgga aaatattccg gtaaatcctt ttaatcacac ctttgaccga aaggattatg
     7741 cacctgcaaa aaaagttgtc ctcagaataa aggaagatat ccacaagata aaggaacgaa
     7801 tgtgcttatc agagcatccc ttcggtacgg taaagtggta tcacggagca aattacctgt
     7861 tatgcaaggg taaagaaaag gccacagcag agctagggct tagcttcctt gcctataatc
     7921 tgcgaagagc aatcaatatg gtaggggtaa ggagattaat agaggcaatg cagggataag
     7981 aaagggggaa tccctttttt tgttttaaaa aagcccgcaa aggcatttaa aattttaagt
     8041 taaaaacgcc gaaaagcctg ataaatcagg cttttcggac aaaatgtggc ggagaagccg
     8101 ggattcgaac ccgggctaga gttgccccta ctaacggttt agcaaaccgt ccccttcagc
     8161 ctacttgggt acttctccaa aaataaattg aataataaat atatcatatt ttattagttt
     8221 taatttatca ggtaataatt ggcggagaga gtgagattcg aactcacggt aggcttgcac
     8281 ctacgccggt tttcaagacc ggtgccttaa accaactcga ccatctctcc aagccagatt
     8341 tacatgacag attacatttt acaatgtttt ttaaactttg tcaacactta aataattaca
     8401 ttttaataca tatgatacac atctatccat atgtactgta tctgcttata aatggcagca
     8461 tttttcgttt ctactgaaat atgttttata ggttgtcatt aatacacaag tcatttgtca
     8521 aaactcaatt ttgttgactt cttaactttt tagctagaaa ataatattcc tcctttttta
     8581 accaaaataa aagaaattat caatatttaa aataccattg ctttttttac aatttaacaa
     8641 ggtcatttca aaataccaac attttttacg attttctttt aaattcagca tgtctcaagc
     8701 tcttatactt attcatattt aaaaatatgt actcaattgt aaactaaaca tattggctct
     8761 ctctattttt tcttatagca tcatttattg cctttatttc ttcagctgaa agtgcatatt
     8821 ttgatttacc accagttatt ggtttaccat atgtagttga actatccctg gaaaacagcg
     8881 atgttataac aacaccatta tcattaccat caagtaatgc cagagaatag cttaaatcac
     8941 ttccgacatc atcaaaagca ttaaaacgaa taataccaga cttttgtaag cacttggaaa
     9001 tatttctgtc taattcattt aaatgattct caatttcttt atgtttaaaa cttacttcat
     9061 ctgttcttaa tatacacttt tctaataatt cttctaaatc ttttccatca tcacaagcca
     9121 taaatttatt atatttttta tataatttat ttatttttat agaattagat atactcatta
     9181 ctaatatagt aataaatata ataagtatta accccattat aattatatat aacatatttt
     9241 cagataaatt taaatcattc atatattgca catccttaaa tatttttcat attatagaaa
     9301 ccaatattaa agtataatac tgattttaac caaaaactaa ttgaacgtca acaaaatcaa
     9361 ttattcaaac accgattttg ctatcaggcc ttcagattat aaagctctac agcacatcta
     9421 ctaatgcttc tttatataat atttttcata aatatagtat acgaccatta taatctgt
//
LOCUS       DAOSDD010000182         9365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2733_length_9365_cov_5.009023, whole genome shotgun sequence.
ACCESSION   DAOSDD010000182 DAOSDD010000000
VERSION     DAOSDD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_9365_cov_5.009023"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PK733_14245"
     CDS             <1..595
                     /locus_tag="PK733_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11737.1"
                     /translation="LENRYTDWGNYYPALTLRNLWDICSFVPAQKLQIEFLNIDRNAE
                     KYKEDDVLAPSKYSYEYALSTVMFANPLCWFEPSALKTEALERYGCMINLHRKYRNEI
                     FAGYIFPIGQRPDGYSWTGFQSHNLSKNSGFIIIYRELNENTRFSIKPLFMEERSVHL
                     QSLSDKTSDITIERYNGDGIEIQLDNVNSFRLYFYQL"
     gene            661..2277
                     /locus_tag="PK733_14250"
     CDS             661..2277
                     /locus_tag="PK733_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11738.1"
                     /translation="MKRILSIVLILCMALSFTACTVSKDSKDSNSKITTGTQTADDQK
                     SGKDSDSDAKTGGLVLPISKNKIEIEYLISQNANNPVKEDWIVFRELEKVTNIKLKLQ
                     VESTDYNAKLNILLASNQLPELIISNVDFGKKNGLEGSLIPVSDYLDKMPNYSKYLAE
                     NPSVKKDISASDGKIYNCVHYGHEVFGMGWLYRKDIFDKNNLSVPKTYDEFYDVCKKL
                     KQIYPESYPLSHRQKNDLLNQIGVQWGTGYKVYYNMDTKKFQYGCLEDNFKEMLMFIK
                     KLYDEKLLDKDWATISTKQWEDSITSDSGFIAYDYLARIKTFNPAMASKNPEFNIEAF
                     LPPKAPDGKALLKTTFDKSGSALEGFAVSKNNKHPEETFKFLDFLYSREGAIITNLGV
                     EGETCKKDKDGNLVWIDDIKSSISPNGTKHYINDFGFIALGTYCVVTPEARMLTREKG
                     YKEAIELYEKNDAATGREPQVSMTSQELSVVQDMASLSGYVDEQVTKFVLGENDLSEW
                     DNFVKEIKKKGVDELLKAYNDALERYNKVN"
     gene            2400..5345
                     /locus_tag="PK733_14255"
     CDS             2400..5345
                     /locus_tag="PK733_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11739.1"
                     /translation="MKSLSKKISIIILNIFILTMAIIPVHGISDVPLREKFNYGNENN
                     KVEYYRKVNEPYYQTLLKEYEENGYNKPDIPAILIESKDISLPEGKELILETELGSSG
                     YPGFKWTDDVPWLEWNFEITDEGLYKIDLEYYMLPGTGNPAARSLLLDGKRPFLEASN
                     IVFYRGWKDEGEPIVNTIGDEVRPSQVEIPGWRIVSLCDALGYYSHPFYLHLKPGSHT
                     IRLEYVDQDMVIGRAWIQPPKDLPAYEKVKEEYVNKGYMKAVKTIEFQAESTAIEKSD
                     PTIRRERNGDPICRPGSSLYRKLNVLGGYRWRKGNQSITWEFTVPQDGLYKIAARAGQ
                     WWNDGLPSYRQIAIDGEVPCRELQEYKFTFNRKWRTEVLDDENGKPLLFYLTKGTHTL
                     TMTVKMGELTPVILSLNDDVILLSNIIRDITKIASSNPDPNYDYDFFRTIPYLKGNME
                     TLIESLQIKYDILKKMSEKLPAMANNLMTIKSQLEGMVKDPFSIARRLNDLNNAQSNL
                     GNWYLSLQNQPLMIDYFLIGNPDEDWGNEQSTAFQKLKATIDNFFVSFKKDYNNIGSV
                     VEENIDIKNTINLWVSRGTEWVELIKEMADEEFTPKTGILVNINVFPAGQLQAGSVNA
                     LMLAIASGRAPDGALGIDSGSPVEFAIRDAVHDLSKFKDFDEMSKRFLPSMFTPFKYN
                     GGVFAVPETMNFIAMIYRKDIISEYGIILPNTREELYNYVLPMLYQNGLQFYYPADFS
                     QFLFQHGAEYYLDDGEKSGLDTPEAYQAFKEVTELYTHYGVPVSASFFNRMRTGEMPI
                     GIGDYNLYIQLSVAAPELAGRWGIAPIPGTARPDGTIDRSNGSIAGQAGVIINQSAKK
                     DDVWEFFKWWTSTPVQTKFARELEAIIGTEARWNTANLEAFTNMAWKKEDLEVIKEQW
                     EWSRGVPVVLGGYYTSRYLNNAWNSIVISGVPVRDALETAVKEINRELRMKREEHGIF
                     DNKAQGVRK"
     gene            5345..6244
                     /locus_tag="PK733_14260"
     CDS             5345..6244
                     /locus_tag="PK733_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010167833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ11740.1"
                     /translation="MAKSKQKPAWRKGLIGYVFMLPFLILFFIFIIIPVFVALGTSFT
                     NYNMLQPVKWIGLTNYKLLFLDDAIFLTAIKNTLIFACITGPIGYFMSFVAAWVINQL
                     RMRNAFALAFYAPSITSGIAMSVIWLYFFSNDRYGLFNNILINLGLIKEPILWTLDAK
                     YILPVIILISIWMSMGTGFLVFLAGLQNIPREYYEAASIDGIKNKFQELWFISMPLLK
                     PQLLFGAINSIVGSFGVFDIAVSVAGMPSPNYAAHTIVAHLYDYAFIRFQMGYASAVA
                     MVLFLITFILGRVVMKMLSPKGE"
     gene            6258..7193
                     /locus_tag="PK733_14265"
     CDS             6258..7193
                     /locus_tag="PK733_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11741.1"
                     /translation="MMESNVVKRIAVNIIKKIQKRRIDNKAPARRYEKTTAADVILHI
                     FMVLLVAFTSLPLVYMISTAFKPLDELFIFPPRYFVRKPTVQNFFDLAISLESSTVPF
                     IRYVYNSLLVTTITVVCTVIVSALGAYGLVKHKPIGSKVVFNFIIAALMFSPHVTQIP
                     RYMVVNKLGLLDSYWALIIPSIAVAYNFFLMKQFTEQFPDELLDSARVDGANEWITFW
                     KIVMPSLTPAWSTLVVFSFVSNWNDYFSPLIYITDNAKKTLPPALQTISGGGSIGRAG
                     AVAAATMLMTLPTIIIFTIMQARVMETMTYSGIKG"
     gene            7207..>9365
                     /locus_tag="PK733_14270"
     CDS             7207..>9365
                     /locus_tag="PK733_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ11742.1"
                     /translation="MKRSKKSGILIVLILTVAISVISFAQETFAASRDYTINDGKRVP
                     IPETYVLKKVINNIGSKGEEPVYLKEPEDLFINSQGYIFIADTGNNRIVKITSDGELV
                     DIFKGSPGKLLKAPKGVYADDNGNMYIADTGNLRIVHLSSEGELVEEFIKPDSELLDE
                     TFTFDSTKLYLSPTGYIYVLKGQSILAMDAYNQFRGYIGQTQIGFSLIDSLLRIFASD
                     QQKKMISKRTAEAYTNITMDEKGTIYATSLDRSEGEIKKLNSVGKNIYRKYGSNDSEP
                     KFLEKLFGSFNRLNLREKSFTFGETRDDNGNPIKPVFKDIAVGENGIITVIEEQTGKL
                     YQYDQEGNLLTVFGGKGDQKGRFVMPSSIVNDNEGRIYVLDRSTGNIQMFEPTEFIKA
                     VHKAVEEYSKGKYNEAYDIWGQILLIDENYQMGHFGLANALYKQEKWKDAMNEYMMAD
                     DRTGYSKAYTEYRYDTFRQYFAIVVLALSAIITALVLFIKWIKGLSLHALEEFRNTHS
                     KKVHVATGLKMAFGVIFHPVETFEEIKNGRGKLNLVPGVIILLSVLAARIFYLFVVHY
                     PLADIDLKDSNIWLEAVKLLLPVITWVIASFAITAILDSESKISEIFLATTYCMVPYI
                     LVTFPLAVLSRILTRNEASFYGFILNCTWIWILLLFFISLRTLNDYSISRTAAAYAIS
                     GGTMVLIWFVSLLGYTLTGKLYQFISGIIYEIRMTWL"
BASE COUNT         3193 a         1514 c         1958 g         2700 t
ORIGIN      
        1 tctggaaaac aggtatactg actggggcaa ttactacccg gcgcttacat tacggaatct
       61 gtgggatatt tgctcctttg ttcctgcaca gaagctgcag atagagtttt tgaatataga
      121 tcgtaatgct gaaaaatata aagaagatga tgtacttgct ccttcaaaat attcatatga
      181 atatgccctt tcaacagtca tgtttgcaaa tcccttatgc tggtttgaac cgtcggctct
      241 taaaacagaa gctcttgaaa ggtatggatg catgatcaat ctccaccgta agtacaggaa
      301 cgaaatattt gcaggatata tttttcccat aggtcagagg cctgatggct attcctggac
      361 aggattccaa tcacacaatt tatcaaagaa cagcggattt attattatat acagggaatt
      421 aaacgagaat acaagattta gtataaagcc attgtttatg gaggaaagaa gtgtacatct
      481 acaaagcctc agcgataaaa cttctgatat aactattgaa agatataacg gagatggcat
      541 tgagatacaa cttgacaatg taaacagttt cagattgtat ttctaccaat tgtaaggtaa
      601 ttacagcatt ttctactgct gttaattata ataaacatat cgtattagga ggtaatattt
      661 atgaaaagaa tactgtcaat agtattgatt ctgtgtatgg cgttaagttt tactgcttgt
      721 acagtatcaa aagacagcaa agacagcaat tcaaagatta ctacaggaac acaaactgca
      781 gatgatcaga aatccggaaa ggattcagat tctgatgcta aaactggcgg gcttgtattg
      841 cctatttcaa aaaacaaaat tgaaatagag tatttgattt cccagaatgc aaacaatcct
      901 gtaaaagagg attggatagt attcagggaa ttggagaagg taacaaatat taagcttaag
      961 cttcaagtgg aaagtacaga ctataatgcc aaacttaata ttttacttgc atccaaccag
     1021 cttcctgaat taatcatatc aaatgttgac tttggcaaga aaaatggatt ggaaggatca
     1081 ttgataccgg tcagcgatta ccttgataaa atgccaaact attcaaaata tctagctgag
     1141 aatcccagtg taaaaaaaga tatatcggca tcagatggca aaatatacaa ctgtgtacat
     1201 tatggacacg aagtttttgg tatgggttgg ctttacagaa aggatatttt tgacaaaaat
     1261 aatctctctg ttccaaaaac gtatgatgaa ttctatgatg tttgtaaaaa actgaagcag
     1321 atatatcctg agagttatcc tttgtcacat aggcaaaaaa atgacctgct gaaccaaatc
     1381 ggtgttcaat ggggaacagg ttataaagta tattacaata tggatacaaa aaaattccaa
     1441 tatggatgtt tagaggataa tttcaaggaa atgcttatgt tcataaagaa actgtatgat
     1501 gaaaagcttc tggataaaga ttgggctact atcagtacaa aacagtggga agacagtatt
     1561 accagtgata gtggttttat tgcatacgat tatcttgctc gtataaaaac cttcaatccg
     1621 gcaatggctt caaaaaatcc tgaatttaac attgaagcat ttttaccgcc gaaggctccg
     1681 gacggcaagg ctttattaaa gacaacattt gataaaagcg gaagtgcttt ggaaggtttt
     1741 gctgtttcaa agaataacaa acatcctgaa gaaacattca agtttttaga cttcctttat
     1801 agcagggagg gtgctataat aaccaacctt ggagttgaag gggaaacctg taaaaaggat
     1861 aaagacggta atctggtatg gattgatgat ataaagagct caataagtcc aaatggtaca
     1921 aaacactata ttaatgattt cggatttata gcacttggaa catattgtgt tgttacgcct
     1981 gaagccagga tgttgacaag agaaaaggga tataaggaag ctatcgaact ttacgagaag
     2041 aatgatgcag caacaggaag agaacctcaa gtaagcatga ctagccagga gttaagtgtt
     2101 gtacaagata tggcttcatt aagcggctat gttgacgaac aggttacaaa atttgttctt
     2161 ggagaaaatg acttatctga atgggataat tttgttaagg aaatcaagaa aaaaggtgtc
     2221 gacgaacttc ttaaagcata taacgatgca cttgaaaggt acaataaagt taactgagag
     2281 aacccaaaat agctaacaca agcaaattaa ggattaaaac caaaacatca tcctgacttc
     2341 tgctagtcag gatgatgttt tacagataac taccatcatt aatttacaag ggggatttta
     2401 tgaaaagcct ctctaaaaaa ataagtatta ttattttaaa catttttata ttaactatgg
     2461 caataatacc ggttcatgga atttccgatg taccgctccg ggaaaaattt aattatggta
     2521 atgaaaataa caaggtggaa tattaccgta aggtgaatga gccatattac cagacattac
     2581 ttaaggagta tgaagaaaat ggatataaca agcctgatat tccggcaatt ttgatagaat
     2641 caaaagatat ttcgctgcct gaaggtaagg aacttatact ggaaacagag ttaggcagca
     2701 gcggataccc tggatttaaa tggacggatg atgtcccatg gcttgagtgg aatttcgaaa
     2761 ttactgatga aggtttatac aagattgatt tagagtacta tatgcttcca gggacaggca
     2821 acccggctgc acgatccctt ttattagacg ggaaaagacc ttttcttgaa gcatcaaata
     2881 tagttttcta cagaggttgg aaggatgaag gagagccaat tgttaatact atcggtgacg
     2941 aagtgagacc cagtcaggtt gaaataccgg gctggcgaat agtaagcctt tgcgatgcat
     3001 taggatatta ctcccatccg ttctatttac acctaaagcc cggaagtcat accattcgtc
     3061 tggaatatgt agatcaggac atggtaattg gcagggcatg gatacaacct ccaaaagatt
     3121 tacctgcata tgaaaaggta aaggaagaat acgttaataa aggctatatg aaagctgtca
     3181 aaactataga atttcaagct gaaagtacag ctattgaaaa gagcgatcct acaattagaa
     3241 gagaaagaaa tggcgatcct atttgcagac ctggatcttc tttatataga aaactaaatg
     3301 ttttgggagg ataccgctgg cgtaaaggaa accagagcat aacctgggaa tttacagttc
     3361 ctcaagatgg tttatataaa atagcagcaa gagcagggca atggtggaac gatggtctgc
     3421 catcatatag acagatagct atagatggag aagttccttg tagggaactg caggaataca
     3481 aattcacttt taatagaaaa tggcgcaccg aagttcttga tgatgaaaat gggaaacctt
     3541 tactttttta tttgactaaa ggaacacata cattaacaat gacagttaaa atgggtgaac
     3601 tgacacctgt tattttatcc ctgaacgatg atgttatatt actttctaat ataataaggg
     3661 atattactaa aatagccagc agtaaccctg acccaaacta tgactatgat tttttcagaa
     3721 caatacctta tttaaaagga aatatggaga ctttaattga aagtctgcaa ataaaatatg
     3781 atatattaaa aaaaatgtct gagaagcttc cggccatggc aaataatcta atgactataa
     3841 aaagccagct tgaaggtatg gtgaaagatc cttttagcat tgccagaagg ttaaatgact
     3901 taaataatgc tcagtctaat cttggaaatt ggtacttaag tttgcagaac cagccgttaa
     3961 tgattgacta tttcttaatt ggaaatccag atgaagattg gggaaatgag cagtctactg
     4021 cttttcaaaa gctgaaagct accattgata acttttttgt ttcttttaaa aaggattaca
     4081 ataacatagg cagtgttgta gaagaaaata tcgacataaa aaatacaatt aatctttggg
     4141 tatccagagg aacagaatgg gttgaattaa taaaagagat ggcggatgag gaatttacgc
     4201 ccaaaacggg catcctggtt aatatcaatg tatttcctgc aggacaattg caggcaggtt
     4261 ctgttaatgc attaatgctg gcaatagcct caggcagggc accagatggt gcattaggaa
     4321 ttgattcagg atctcctgtt gagtttgcta taagagatgc agtacatgat ttaagcaaat
     4381 ttaaggattt tgatgaaatg tcaaaacggt ttctgccaag tatgtttaca ccttttaaat
     4441 ataatggagg tgttttcgca gtcccggaaa ctatgaattt tattgcaatg atataccgta
     4501 aagatataat cagtgaatac ggaataatcc ttcccaacac aagagaggaa ttatataatt
     4561 atgtccttcc tatgttatac caaaacggac tgcagtttta ttatccagct gactttagcc
     4621 agtttctttt ccagcatggt gcagaatact atctggacga cggagaaaaa tcaggcttgg
     4681 atacaccgga ggcctatcag gcttttaaag aagtaacaga gctatatact cattatggag
     4741 tgccggtatc tgccagtttc tttaaccgta tgcgcacagg ggaaatgcct ataggtatag
     4801 gagattacaa tctatacata caactatctg tagctgcacc agaacttgct ggaagatggg
     4861 gtattgcacc tataccggga acggctcggc ctgatggcac aatagatcgc tcaaatggca
     4921 gtatagcagg ccaagcaggg gttattataa atcagtcggc gaagaaagat gatgtttggg
     4981 aattctttaa atggtggacc agcacgcctg ttcagaccaa attcgcaagg gaacttgaag
     5041 ccataatagg tacagaagct cgttggaata ctgctaacct ggaagctttc acaaacatgg
     5101 catggaaaaa agaagatttg gaagtaataa aggaacagtg ggaatggtcc agaggtgttc
     5161 ctgtagttct tggaggatat tataccagca gatatttgaa caatgcatgg aacagtattg
     5221 taataagtgg cgttccagta agagatgcac ttgaaacggc ggtaaaggaa ataaatcgtg
     5281 aacttagaat gaaaagggaa gaacatggta tatttgacaa taaagcacag ggggtgagga
     5341 aataatggca aaatcaaaac aaaaaccagc ttggagaaaa ggtttaatag gttacgtctt
     5401 tatgctgcct tttctcatcc tgttttttat atttataata atcccggttt ttgttgcttt
     5461 aggaacaagc tttacaaact ataatatgct tcaacctgta aaatggattg gactaaccaa
     5521 ctataagctg ctttttttag acgatgcgat ttttctaaca gcaattaaaa acaccctgat
     5581 atttgcctgt ataacgggtc ccataggtta ttttatgtct tttgttgcag cctgggtcat
     5641 aaaccagctc cgtatgcgca acgcttttgc tttagctttt tatgccccgt ctattacaag
     5701 tggtattgct atgtcagtta tttggttata tttcttttca aatgatagat atggtttgtt
     5761 taacaatata cttataaacc ttggtttaat aaaagaacca atattatgga ctcttgatgc
     5821 aaaatatatc cttccggtaa ttatacttat ttccatatgg atgagcatgg gcacagggtt
     5881 tctggtgttt cttgctggtc ttcagaacat tcccagagaa tactatgaag cagcttccat
     5941 tgatggtata aaaaataaat tccaggaact atggtttata agcatgccgc ttcttaagcc
     6001 gcagctgtta ttcggtgcaa taaactcaat agtaggttct tttggagttt ttgatatagc
     6061 agtatcagta gctggcatgc caagccccaa ctatgcggct catactattg tggcacatct
     6121 ttacgactac gcatttataa ggttccaaat gggatatgca tcagcagtgg ccatggtatt
     6181 atttttaatt acatttatat tgggacgcgt tgttatgaag atgctttcac ctaaagggga
     6241 ataagaaagg gaggtttatg atggaaagta atgtagtaaa acgaattgca gtaaatatta
     6301 taaaaaagat acaaaaaagg cggattgata ataaagcacc tgcgagaagg tatgagaaaa
     6361 caactgcagc tgacgtaatt cttcatattt ttatggtgct gctggtagct tttacatctt
     6421 taccgctggt ttatatgatt agtactgcgt ttaaaccatt agacgaactg ttcatattcc
     6481 cgccacgtta ttttgtcagg aaaccaacgg ttcaaaactt ttttgacctt gcaatctccc
     6541 ttgaaagttc aacagttcca tttatacgtt atgtatacaa cagcctgctt gtaacaacta
     6601 ttacagttgt atgtactgtt attgtatcag cacttggtgc atacggactt gttaagcata
     6661 agccaattgg ctcaaaagtg gtgtttaatt ttatcatagc agcattgatg ttttcgcccc
     6721 atgtaactca gataccccgt tatatggttg taaacaagct tggactcctg gacagctatt
     6781 gggcactgat tataccgagt attgctgtag cttacaactt tttcctaatg aagcagttta
     6841 cagaacaatt ccctgatgaa cttctggatt ctgcaagagt agatggtgcc aatgaatgga
     6901 tcacattttg gaaaatcgtg atgccttctc ttacaccggc atggtctaca ctggtagtat
     6961 tcagctttgt cagcaactgg aatgactatt tttcaccact tatatatata acggataatg
     7021 caaaaaaaac tttgccgccg gcacttcaga ctatttcagg cggaggaagt attggcaggg
     7081 cgggtgcagt cgcagcagct acaatgctta tgactttgcc tactataatt atatttacaa
     7141 taatgcaggc aagggtcatg gaaaccatga cttattcagg aataaaaggg taaaggagga
     7201 aataccgtga aaagaagcaa aaagtcaggc atattaatcg tattaatcct aacggtagca
     7261 atatcagtga tatcctttgc acaagaaact tttgcagcaa gtcgtgatta cactataaat
     7321 gatggaaagc gtgtacctat ccctgaaaca tatgtattga aaaaagtcat aaataatata
     7381 ggaagcaagg gagaggagcc cgtataccta aaagaaccgg aggatttatt tataaattca
     7441 caggggtata tttttatagc ggacacagga aataatagga tagtaaagat tacatctgat
     7501 ggtgaattgg tggatatatt caaaggttca ccgggaaaac tcttaaaagc tcctaaagga
     7561 gtctatgcag atgacaatgg caatatgtac atagcagaca caggaaatct aagaatagta
     7621 cacctttcat cagaaggaga gcttgtagag gaattcataa aaccggattc agagcttctg
     7681 gatgaaacct ttacttttga ctcaacaaaa ctctatttaa gtcctacagg ttatatttat
     7741 gtgttaaaag gacagagcat acttgcaatg gatgcatata accagttcag aggctacatt
     7801 ggacaaactc agatagggtt tagtctgata gactctcttc ttagaatatt tgcctcagac
     7861 caacagaaaa agatgataag caaaagaaca gctgaagcgt atacaaacat taccatggat
     7921 gagaagggca ctatttatgc cacaagtctg gatagatcag agggagagat aaaaaagctc
     7981 aactctgtag gtaaaaacat atacagaaaa tatggaagta atgactctga acctaaattt
     8041 ttagaaaaat tattcggttc ttttaataga ctgaatctta gagaaaagag ttttaccttt
     8101 ggcgagacaa gagatgataa tggaaatcca ataaaaccag tattcaaaga tatagctgtt
     8161 ggagaaaacg gcataattac ggtgatagaa gaacagacag gtaagcttta ccagtatgat
     8221 caggagggaa acctgctgac agtgttcggt ggcaaaggtg atcaaaaagg taggttcgtt
     8281 atgcccagtt ctattgttaa tgataatgag ggtagaatat atgtactgga caggtcaacg
     8341 ggaaacatac agatgtttga acctactgaa tttataaaag cagtacacaa agctgtagaa
     8401 gaatattcaa aaggaaaata caatgaggcg tatgatatct gggggcagat attattgata
     8461 gatgaaaatt atcagatggg tcattttggt ctggctaatg cattgtacaa acaggaaaaa
     8521 tggaaggacg ctatgaatga atacatgatg gcagatgaca gaacaggtta ctcaaaggca
     8581 tatacagaat atagatacga cacattcaga cagtacttcg ccattgttgt gctggcattg
     8641 tcagctataa ttacagcact ggtactattc atcaaatgga taaaaggttt gtctttacat
     8701 gcccttgaag aattcagaaa tactcattct aaaaaagttc atgtagcaac aggacttaag
     8761 atggcttttg gtgtgatatt ccatcccgtt gaaacttttg aggaaataaa gaatggaaga
     8821 ggaaagttga atcttgttcc gggagttata attcttttat cagtgctggc agcaagaata
     8881 ttttacttgt ttgtagtaca ttatcctctg gctgatatag acttgaagga ttcaaacata
     8941 tggcttgaag ctgtaaagct cctcttgcct gttattacat gggtaattgc cagctttgct
     9001 attacagcca tacttgacag tgagtcaaag ataagtgaga tatttcttgc aactacttac
     9061 tgtatggtac catatatact ggttacattt ccgctggcag tgctgtcaag gatacttaca
     9121 aggaatgaag cttcatttta tgggtttata ctgaactgca catggatatg gatattattg
     9181 ctgtttttca ttagcctcag gacattaaac gactacagca tatcaagaac agctgcagct
     9241 tacgcaataa gtggcggcac tatggtttta atatggttcg tgtctcttct tggatataca
     9301 ttaacgggga aactttacca gttcataagc ggtataatat acgaaatacg tatgacatgg
     9361 cttta
//
LOCUS       DAOSDD010000183         9277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2772_length_9277_cov_5.058013, whole genome shotgun sequence.
ACCESSION   DAOSDD010000183 DAOSDD010000000
VERSION     DAOSDD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2772_length_9277_cov_5.058013"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..683
                     /locus_tag="PK733_14275"
     CDS             159..683
                     /locus_tag="PK733_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOJ11743.1"
                     /translation="MRYIFTALYYLIARHLPASDMPYSLGARSIRGFLCRRIFKHAGK
                     NVNIEHGAYFGSGRDIEIGDNSGLGFNCRVYGPLKIGNDVMMGPDVMIFTQNHETIRL
                     DIPMRLQTAPKLPVVIGDDVWIGARVIILPGKTIGKGVIIGAGAVVTKDIPDYAVVGG
                     NPARIIKFRDGRDK"
     gene            701..1798
                     /locus_tag="PK733_14280"
     CDS             701..1798
                     /locus_tag="PK733_14280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ11744.1"
                     /translation="MKVLYLLNHAGKAGTERYVQTLIEKLNNKRIKAYFAYNEEGLLV
                     ERLKNAGVDTFQINMRNPFDIKAAYKLSGLCKKLGINLIHTQFLRENYIAVLSRIFNP
                     RVKAMYTNHFVIPNNMIQKFCNRLVTPFDSNIITVCNKGREMLIANGNKASRIKVIFN
                     GVDTSYWRESVESNMRNELGITDNEFVMLCASRFAHDKGHKYLVDSINKLKKMTDKKF
                     KCVLAGDGPLLDDVKKQVSDLGLNNEIIFTGFRSDIKNLFMGSDLYVNSSEHEALSFL
                     IIEALASGLPVIATDMGGNSDIINAETNCGILVEYNNPESMAKGILKIMSDENMLKKL
                     KKNALKAVEDKFNLDKVVDRTYNLYVESVGS"
     gene            1902..2396
                     /locus_tag="PK733_14285"
     CDS             1902..2396
                     /locus_tag="PK733_14285"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HOJ11745.1"
                     /translation="MLIDEKGRLFGKVNIVDLIVVAVIVLGVLGVAYKYVKSSSSLFK
                     KTEDLQIVFYNEDLPKYVADSIKVGDKVKDSVKNTVFGEVASVETDNSVVFAPNDKGE
                     LTQTTRPGYVSMKLTVKGKGIYTDTGVIFNNTDYYVGRSLELRAGNGVIWTRVSEIKR
                     ASEQ"
     gene            2479..4008
                     /locus_tag="PK733_14290"
     CDS             2479..4008
                     /locus_tag="PK733_14290"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOJ11746.1"
                     /translation="MVSDSVVIRFLNVIAGYLLKVFYNSSTYRVLKSIASLFRRCFGS
                     SFTINILKREGTLLKVWDKSKTYKNFSRLLHSPTKFMRIWFEKRQHVFQNSHVHKLII
                     IAADNLHVLTSLALFATFIIPHSLWYNIFATMMAFALLLLLFIKTIINKNISLNFKYT
                     SLYLAIFVLSVLLAQIFSIFPGLSLRFLVFHLTCLIFVFVLVNSITSREQLTTVIESN
                     LMGVAFVGLYAIFQRIKGVPVNAAQTDLSLNAGMPGRVYSTMENPNNLAEILIMLIPF
                     YVAVFLSSRTFIKKFIIFGMALPPLAALALTYSRSSWIGFAVAFLVFVILTNWRLVPL
                     FILAGVAMIPVLPQTVYNRILTIFDTRDSSVGYRFLIYKTIGPMIEDYWFTGVGLGSD
                     TFMKVVQNYPLHTKVIPPHTHNLFIQVWIETGLIGILSFLGFIVSTIKKGVKRIALEN
                     DKCVKYNIIAGISSLAGILTVGVAEYVWYYPRVMIIFWVVTGLLLAALNLQKNDGTVN
                     E"
     gene            complement(4101..5414)
                     /locus_tag="PK733_14295"
     CDS             complement(4101..5414)
                     /locus_tag="PK733_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ11747.1"
                     /translation="MKKVLMIAYSFPPAGGPGVQRTSKFVKYLRTYGWEPVILTRDLI
                     NMPLKDESLVKDIPDNINIIRTKAWDLNNGTGFPGLVGKFISRKVLIPDSERLWEIFS
                     RKAAEKFIEEQNIQLIYTTSLPYSSHLMAMYLKKTYPDIPWVADFRDEWINNPYILDN
                     PYNPLRMNIERKMEHKVLQKADYLITNTPVMLKNFIKNNPDIQLEDKFFVIPNGYDWD
                     DFKHIVPEPDKVRPGSSVEVETKVWAEARNDKFTITYTGSFYGRRKPDIFFDALGQLV
                     KENEVNKDNIHIKLIGSFKQEQIKELSEIHGLQGIVEVFSYMDHDECIQNMTQSDCLL
                     LIEGAGPGAEAFYTGKVFEYMVSGRPILAIIPERGAAAQLIRETQTGLISDCTDLNGT
                     KKNIKSLYSSWLEEINIYFPNKEEIRKYERNTLTAELAKVFDKAL"
     gene            complement(5411..6703)
                     /gene="wecC"
                     /locus_tag="PK733_14300"
     CDS             complement(5411..6703)
                     /gene="wecC"
                     /locus_tag="PK733_14300"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HOJ11748.1"
                     /translation="MVFIKKKICIIGLGYIGLPTAAMFATHGHQIVGVDVNEKVVDAL
                     NNGKITIYEPYLDILVQAAVTSGNLTGKTVPEEADAFIISVPTPITSEKTADMSYVKI
                     AAESIVPYLREGNLVILESTSPPGTVNNLLLPLLEKSGLKIGEELLVAHSPERVLPGK
                     ILIELVENPRIIGGINRKSAEAVRDLYRTFVKGEIYLTNATTAEMCKLMENTYRDVNI
                     ALANELSILCEDIGISAWEVIQLSNKHPRVNIHQPGPGVGGHCLAVDPWFIVEKDPDT
                     AKMIRLGRQINDSMPMHVLERAKAILTGVPETISQMKVTILGISYKPNIDDTRESPII
                     ELINLFEKESVCELSIYDPHVTNYKYMCKDIYSAAEGSHLVILGVNHEAFGKVDFELL
                     YKKMALPNILDTRNFWDRKALGKLGFNYYLLGDGSQDK"
     gene            complement(6923..7345)
                     /locus_tag="PK733_14305"
     CDS             complement(6923..7345)
                     /locus_tag="PK733_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11749.1"
                     /translation="MEKIDKALELIEKLYFVIQDSKSELKGEIQDVRTELKSDIQDVR
                     TELQGEIQSVRTELKEEIQGVRTELKSEINKIQITLENDIKPDIKCLYEGYSQVYKKV
                     ENIEVKVDEISQKVKSQDVRIQVIEGRNKGKKKIKQET"
     gene            7687..8499
                     /locus_tag="PK733_14310"
     CDS             7687..8499
                     /locus_tag="PK733_14310"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11750.1"
                     /translation="MKNFEEKMNLFKNNKVVNWHEHVWFKPGTKELNEEGCDKLVETA
                     SQVYMDYLVCSLPIPGGMPGPDDIRLCNDTVAKAMKRYPKMIKGMAFINPGYTREALD
                     EIDRCINELGMLGIKLYNHYFASDPIVRPIIEKCIDLDIPMLYHAGKLTVHPESQPFL
                     SNGVHFAKAAKEYPEAVIIHGHIGGGGDWHWSLKAMSDCPNVFTDISGSVHDAGIIET
                     TYKMMGADRMLFATDGTFSTGIGKLLGADIPDSDKVKILNNSRFAKYLERGN"
     gene            8502..>9277
                     /locus_tag="PK733_14315"
     CDS             8502..>9277
                     /locus_tag="PK733_14315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11751.1"
                     /translation="MFIDVNTYIGHWPFRQVGNNTAEELVKLMDEEGIDMACVSNINS
                     IFYRNVQDGNEELAEEVKPYNNRLIPFAIINPAYPGWEKDFKRCIDELGMKGLELYPS
                     YHQYSLTDSTAVELLNMAADRNIPVHLPCAIENLRQRHWLDTKEDLSADSVKQVLALC
                     PNTNFIITNGPTHSIASYLQTASVDRQGKVYYDFCRVDMLNSTFDRFVEAVGADRVVF
                     GSVMPLQYLETQFVKLQFSKLGNEDKEKIMYKNLKELLKL"
BASE COUNT         2872 a         1543 c         1813 g         3049 t
ORIGIN      
        1 ttcaaatcgt aaattatata aatacaatct agggaataaa acctaggagt tttatccgta
       61 tgcatttatt gaaatggtgt ggagcaattt gattggcgaa ttgcccctat gttataataa
      121 ctataaaata tgaaatgaat aacatattga ggaataatat gcgctatata tttactgcat
      181 tatattattt aatagccaga catctgcctg cttcggacat gccttacagt ctgggcgcca
      241 ggagcataag aggttttttg tgccgcagga tattcaagca tgcaggtaaa aatgttaata
      301 ttgagcatgg ggcatatttc ggttccggca gggacataga aataggcgat aattcaggat
      361 tgggatttaa ctgcagggta tacggccccc tgaaaatagg taatgatgta atgatggggc
      421 cggatgtcat gatatttacg cagaaccatg aaactatacg gctggatatt ccaatgaggc
      481 ttcaaacagc accaaaactt cctgttgtta ttggggacga tgtatggata ggagcacggg
      541 tgattattct tcccggaaaa actataggaa aaggagttat aataggcgcc ggagcagtag
      601 tgacaaaaga tatccctgat tatgccgtag taggaggaaa ccctgctagg ataatcaaat
      661 tcagggatgg cagggataaa tagttggagg tgggaatatt ataaaagtat tatacctttt
      721 aaatcatgcc ggaaaggccg gtacagaaag atatgttcag acccttattg aaaagcttaa
      781 caataaaagg ataaaagcat attttgcata taatgaagaa gggttgcttg tagaaagatt
      841 gaaaaatgca ggtgttgata cttttcaaat caacatgaga aacccttttg atataaaggc
      901 agcctataag ttaagcggtt tatgcaaaaa gttgggaatc aatttaatac atacccagtt
      961 tttaagggag aactatatag cagttctctc aaggattttc aatcccagag ttaaagctat
     1021 gtatacaaat cattttgtaa ttccaaataa tatgatccaa aagttttgca atagattggt
     1081 aactcctttt gattcaaaca ttataactgt atgcaacaaa ggtagggaaa tgctgattgc
     1141 aaatggcaac aaggcatcaa ggattaaagt tatatttaat ggtgtagata cttcctattg
     1201 gagagaatct gttgaatcta atatgcgaaa tgaactgggg attacagata atgagtttgt
     1261 aatgctttgt gcatcaagat ttgcgcatga taagggtcat aaatatcttg tagattctat
     1321 taataagtta aagaaaatga cagataaaaa gttcaagtgc gtacttgctg gagatggacc
     1381 tctgcttgat gatgttaaaa aacaggtatc agatttaggc cttaataatg aaataatatt
     1441 tacagggttc cgtagtgata taaaaaatct ttttatggga agcgatctat atgtaaattc
     1501 atcggaacat gaggctctca gctttcttat tattgaagca cttgcttcag gtcttccggt
     1561 tatagcaacc gatatgggtg gcaactcaga tattattaat gctgaaacaa actgtggaat
     1621 tcttgtggaa tataataatc ctgaatctat ggctaaggga attttaaaga ttatgtcaga
     1681 tgagaatatg ttgaagaagt tgaaaaaaaa tgctttaaaa gctgttgaag ataaattcaa
     1741 tcttgataaa gtagtagata gaacgtataa tctatatgtg gaaagtgtgg gtagctagcc
     1801 tggctgtgat atgctacagc ctgtaaggtt ataggtcaga tgactttcct gccataattt
     1861 taatttatat aaaatacgaa agtatgaaag gaagaaaaca aatgcttatt gatgaaaagg
     1921 gaagattgtt tgggaaagta aatatagtag atttaattgt tgtagctgta attgttttag
     1981 gagtattggg agtagcatac aagtatgtta aatcatcttc gagcttgttt aagaaaaccg
     2041 aggatttaca aatagttttc tataatgagg atttgccaaa atatgttgca gattctataa
     2101 aggttggaga caaggttaaa gactctgtaa aaaatacggt ttttggagaa gtagcatccg
     2161 tagaaacaga taattcagtg gtttttgcac caaatgacaa aggtgaactg acacaaacca
     2221 caagaccagg gtatgtatct atgaaactta ctgtaaaagg taaaggtata tacactgata
     2281 ctggtgttat ttttaataat accgattact atgtgggcag atctctcgaa ttaagagcag
     2341 ggaatggtgt tatctggacc cgtgtcagtg agattaagcg tgcctcggaa cagtaattta
     2401 acaatggttc agcaatggct taataatagt ttaacaatag ctcaacaata gttcattagt
     2461 ggaaaggggc cataattatt ggtatcggat agtgttgtta ttagatttct caacgtgatt
     2521 gccggttatt tacttaaggt tttttataat agctctacat atagggtgct caaaagcatt
     2581 gcaagcttgt tcagaagatg ctttggttca agtttcacaa taaatatact aaaacgggaa
     2641 ggtactcttt taaaagtatg ggataagagc aaaacatata aaaatttttc gagattgctg
     2701 cattcaccaa ctaaatttat gagaatatgg tttgaaaaac gacagcatgt ttttcagaat
     2761 agtcatgttc ataaactcat aataatagcg gcggataatt tacatgtttt aacgtctttg
     2821 gctttatttg caacttttat tatacctcat agtttgtggt ataatatttt tgcaacaatg
     2881 atggcgttcg cattattgct actattattt attaaaacta ttataaataa aaatatatct
     2941 ttaaatttta agtacactag tttatatctg gcgatttttg tgctatcagt tttgctggca
     3001 cagatatttt ctattttccc tggccttagc cttaggtttt tagttttcca cctgacctgt
     3061 ctgatttttg tgtttgttct ggtaaactct attacaagca gagaacagct tacaactgtt
     3121 attgaaagta atcttatggg ggttgcattt gtaggattat atgcaatttt tcagagaatt
     3181 aaaggtgtgc ctgtaaatgc cgcccaaact gatttaagcc ttaatgcagg aatgcccgga
     3241 agagtgtatt ccacaatgga aaaccccaat aacctggctg aaatactcat aatgttgatc
     3301 cctttttatg tagcggtatt tttgagttcc cgtacattta ttaagaagtt tattatattt
     3361 ggaatggctt tgcctccatt ggccgccctt gccctgacct actcaaggtc atcatggata
     3421 ggctttgcag ttgcatttct tgtgtttgtt atacttacaa actggagact tgtaccttta
     3481 tttattctgg ctggagtcgc catgatacct gttctgcctc aaacagtata caacaggata
     3541 ctaaccatat ttgacacaag agatagctct gtaggctaca gatttttaat ttataaaacc
     3601 atcggaccta tgattgaaga ttattggttt acaggagtag gattaggcag tgatactttt
     3661 atgaaggttg tgcaaaacta tcctttacat acaaaagtta taccgcccca tactcacaat
     3721 ttattcattc aggtttggat agagacaggg ctaataggga tactctcctt tctcggattt
     3781 atagtgagta caatcaaaaa aggtgtaaaa agaattgcat tagaaaatga caagtgtgtg
     3841 aaatacaata taattgctgg aatatcttct ttagcaggta tcttaacagt aggggttgct
     3901 gagtatgttt ggtattaccc cagagtaatg attatttttt gggttgtaac aggcttgctg
     3961 ctggccgcct taaatttgca gaagaatgat gggacagtta acgagtaaat aatcaacaat
     4021 ttattgggaa catgcctcaa cataaaacgg gtactacctg ctcgagggag gtgtatcccc
     4081 tgaccatgat aatagtaaaa ttaaagtgct ttatcaaaca cctttgccaa ttctgcggta
     4141 agggtgtttc tttcatactt tcttatttct tctttattcg ggaaatagat atttatttct
     4201 tccagccatg aactatacaa gcttttaatg tttttctttg tgccgtttaa atcagtgcaa
     4261 tcagatataa ggcctgtttg ggtttccctg ataagctgag ctgctgctcc ccgttcaggt
     4321 ataattgcaa gtattggcct tccggaaacc atgtactcaa ataccttgcc ggtgtagaaa
     4381 gcttcagctc caggtcctgc tccttcaata agcagaagac agtctgattg ggtcatgttc
     4441 tgtatacatt catcatgatc catatatgaa aaaacttcta caattccctg taaaccatga
     4501 atttctgaca attcctttat ttgttcctgt ttaaagctgc caattaattt tatgtgtata
     4561 ttgtccttgt taacttcatt ttcctttacc aactgtccaa gggcatcaaa aaatatatca
     4621 ggctttctcc tgccataaaa tgatcctgta tatgtaattg taaacttatc atttcgagcc
     4681 tcagcccaaa ccttagtctc aacctcaacc gaactgccag gtcttacctt atctggttcc
     4741 ggaactatat gcttaaagtc atcccagtca tatccgttgg gaattacaaa aaacttatct
     4801 tcaagttgaa tatccgggtt gtttttaata aaatttttga gcattaccgg tgtatttgtt
     4861 atcagataat ctgctttttg cagaacttta tgttccatct tcctctcaat attcattctg
     4921 agaggattat aggggttgtc aagaatatag gggttattta tccactcatc cctgaagtct
     4981 gcaacccatg gtatatcagg atacgttttc ttaagataca tagccataag gtgagaacta
     5041 taaggcaggg aagtcgtata tattagctgt atattctgct cttcaataaa tttttctgct
     5101 gcctttcgag agaatatttc ccacagtctc tcactgtcag gtattaagac tttgcggctt
     5161 ataaacttgc cgacaagacc tggaaaacct gttccattgt tcaaatccca cgcttttgtg
     5221 cgaataatgt ttatattatc gggtatatct ttaacaagag attcatcttt taaaggcata
     5281 ttgattaaat ctcttgtaag aattacaggt tcccagccat aggttcgcag atattttaca
     5341 aactttgaag ttctctgaac tcccggccct cccgcaggag gaaaggaata ggcaatcata
     5401 agtacttttt tcatttatct tgactcccat cacccagaag gtaataattg aaccccagct
     5461 tgccaagagc ttttctatcc cagaagtttc tggtatccag aatgttgggc agagccattt
     5521 ttttgtaaag caactcaaaa tctactttgc caaaggcttc atggttaact cccaaaataa
     5581 ctaaatggct tccttcagcc gcagaatata tgtctttaca catatattta taattagtta
     5641 catgagggtc atatatactt aattcacaga cactttcctt ttcaaaaagg ttaataagct
     5701 caattattgg actttccctt gtgtcgtcaa tatttggttt atatgatatc ccgagtattg
     5761 taactttcat ttggctgatt gtttcgggca cacctgttaa tattgctttt gccctctcaa
     5821 ggacatgcat tggcatactg tcatttatct gtcttccaag cctgatcatt tttgctgtat
     5881 ctggatcttt ttcaacaata aaccatgggt ctactgccag gcaatgtccc cctacacctg
     5941 gtcccggttg atgaatatta accctgggat gcttgttgga tagctgaata acttcccatg
     6001 cacttattcc aatatcttcg caaagaatgg aaagctcatt tgccaaagct atgttaacat
     6061 ctctgtaggt attctccata agcttgcaca tttcagctgt agtagcattg gttaagtaaa
     6121 tttcaccttt tacaaaagtt cggtaaaggt ctcttactgc ttctgcggat ttccggttaa
     6181 tgccgccaat aatccttgga ttttcaacaa gctcaataag tatcttacca ggtagtactc
     6241 tttcaggtga atgggcaact agaagttctt ctcctatttt caagccggat ttttcaagta
     6301 ggggtagcag aaggttattt actgtccctg gaggtgaagt ggattcaagg attactaggt
     6361 tcccttctct taaatatgga acaatagact cggcggctat ttttacatag gacatatctg
     6421 cagttttctc agaggtaatt ggggtaggca cggaaataat aaatgcatcg gcctcttccg
     6481 gtacagtttt gcctgttaaa ttacctgagg ttactgctgc ctgtaccaaa atatccaggt
     6541 atggttcata aatagttatt ttcccgttat taagagcgtc aacgaccttc tcatttacat
     6601 caacaccgac tatttggtgc ccgtgagtgg caaacattgc ggcagtcggc aagccaatat
     6661 atcctaaacc gataatacaa attttctttt ttataaaaac cacgtccttt tttcatatat
     6721 aacgtatata gatcccataa gaaacaagat gcattttttg tgtattcact acggtatcat
     6781 taacatgtaa attttataat ataccatctt ttatacgcta tacaataaat gaaatatgtg
     6841 aaaatcataa atttatcttt gaaattaagt ttagcacttc taaattacac tgtcaattat
     6901 tgaaaatgtg ccggggttat catcatgtct cctgttttat cttctttttg cctttattcc
     6961 tgccctcaat aacttgaatt ctaacatctt gactcttgac cttttgggat atttcatcaa
     7021 ctttaacttc tatattttcc actttcttat agacttgcga atagccttca tacaaacatt
     7081 ttatatctgg ctttatatca ttttccaagg tgatttgaat cttatttatt tcacttttga
     7141 gttcagtcct cacgccctgg atttcttcct taagctcagt cctcacgctt tgtatttcac
     7201 cttgaagctc cgtcctaaca tcttgtatat cacttttgag ttcagttcta acgtcttgga
     7261 tttcaccttt gagttcagac tttgaatctt gaattacaaa atatagcttc tcaattagtt
     7321 ctagcgcctt gtctattttt tccacatgca tccacctttc agagagttaa ttctctcaat
     7381 aattatatat tgagagaaag gaaaagtcaa ccttaataat gtaataaatg taacattatg
     7441 gattatattt cactgcactt aatgatatag gatagtcatg taataaaaat gatattgttt
     7501 taaagcaggg tatagagtat aattttggtg aaatattttg ataattttga tataatgaaa
     7561 taaaaagtag gggaatattt attttcaatt atatttggtt tcctttagac agagtgtatg
     7621 gcatatatta ttgagatttt tattgtttta gaggtttcct attgtttgct taaggaggga
     7681 aagagattga aaaactttga ggaaaagatg aatcttttca aaaataataa agtggtaaac
     7741 tggcatgagc atgtatggtt taagcctggc acaaaggaat tgaatgaaga aggctgtgac
     7801 aagttggtag aaacagcaag ccaggtttat atggattatc tggtttgttc attgccaata
     7861 cctggaggaa tgcctgggcc ggatgatata aggttgtgta acgatactgt tgcaaaggct
     7921 atgaagcgct accctaaaat gataaaaggg atggctttta taaatccagg atatacaagg
     7981 gaggcgttgg acgagattga caggtgtata aatgaattag gtatgctggg tattaaactg
     8041 tacaatcatt atttcgcatc tgatccaatt gtcaggccaa taatagaaaa gtgtattgac
     8101 ctggatatac caatgcttta tcatgcagga aaactcactg ttcaccctga atcacaaccg
     8161 tttttatcta atggagtgca tttcgcaaaa gcagctaaag aatatcctga agctgttatt
     8221 atccatggcc atattggcgg tggcggtgat tggcattggt ctcttaaggc tatgtcagat
     8281 tgtcccaatg tttttacaga tataagcgga agcgtacatg atgcaggtat aatagaaaca
     8341 acatataaaa tgatgggagc agacagaatg ttgtttgcta cagatggtac attttctacc
     8401 ggaattggaa aattattagg tgctgatata cctgattcgg ataaagtaaa gatacttaac
     8461 aattcaagat ttgcaaagta tcttgaaagg gggaactagt aatgtttata gatgtaaaca
     8521 catatatagg ccattggcct ttcaggcagg ttggcaataa tactgcggag gaacttgtaa
     8581 agttaatgga tgaagaagga attgatatgg cctgcgtttc caatattaat tccattttct
     8641 acaggaatgt tcaggatggt aacgaagaat tggcagaaga ggttaagcca tataataatc
     8701 gcttaatccc ctttgcaatt atcaaccctg cctatccggg atgggaaaag gattttaagc
     8761 gttgtataga cgaactgggt atgaaaggac tggaattata cccttcatac catcagtaca
     8821 gcttgactga cagtactgca gtagagcttc ttaatatggc tgctgataga aatattcccg
     8881 ttcatcttcc gtgcgctatt gaaaacttac gacagcgtca ttggctggat actaaagagg
     8941 atttatctgc tgatagtgta aagcaggtat tggcactatg tcccaatacg aactttataa
     9001 taacaaatgg tcccactcat agcattgcaa gttaccttca aacagcatca gtcgataggc
     9061 agggaaaggt atattatgat ttttgtagag ttgatatgct gaattctacc ttcgacagat
     9121 ttgttgaagc tgtaggagct gatagagtag tatttggttc ggttatgccg ttacaatatc
     9181 ttgaaactca gtttgtaaag ctccagtttt ctaaattagg aaatgaagat aaagagaaga
     9241 taatgtacaa gaatttgaaa gagttactta agttgtg
//
LOCUS       DAOSDD010000184         9172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2814_length_9172_cov_4.824175, whole genome shotgun sequence.
ACCESSION   DAOSDD010000184 DAOSDD010000000
VERSION     DAOSDD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2814_length_9172_cov_4.824175"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..328)
                     /locus_tag="PK733_14320"
     tRNA            complement(254..328)
                     /locus_tag="PK733_14320"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Cys,seq:gca)
     gene            complement(477..1589)
                     /locus_tag="PK733_14325"
     CDS             complement(477..1589)
                     /locus_tag="PK733_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOJ11752.1"
                     /translation="MKRRMLRLIAFLMLTSTVLLVLSTGCSNWAGFSGGKYHKYTYEF
                     IGTFDTVIQFMGYAENEKQFEELAKKGQARFEELHKLFDIYNIYDGINNIKTINDNAG
                     IKPVEVKQEIIDIIQFSKEWYDKMGGVVNIALGPVLSIWHTYREEGMRDPANAKIPDI
                     VLLKKAELKTDIKKVEVDAVKKTVFLKETGMSLDMGAVAKGFATEIVARELEAAGYTS
                     FIISSGGNVRVVGKPLDGTRSKWGIGIQNPDGNAIIPEGEALDTVFVTDTSVVSSGDY
                     QRFYEVDGKRIHHLIEPSTLMPANYYRAVTVMTKDSGQADFLSTTVFLLPYEKSRELV
                     ESLDGVEALWIMQDGSIAVTDNMKKSMKKLGGATGK"
     gene            complement(1744..2292)
                     /locus_tag="PK733_14330"
     CDS             complement(1744..2292)
                     /locus_tag="PK733_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11753.1"
                     /translation="MKKIIAISLAVVTLTIILFTGCSSSAAVKTGLGQVTSISKSADA
                     AADKDGAAQVDTIMAAVSVDSSGKIVSVTIDSAQTTVLFDATGKIKSDLNAEQKTKRE
                     LGKDYGMIKGSKIGKEWYEQIDELQKWMVGKTIDQIKTMKVKKVDDTHTNVPDEPDLT
                     SKVTLSVEDYIAAVEEAVKNAK"
     gene            complement(2365..3303)
                     /locus_tag="PK733_14335"
     CDS             complement(2365..3303)
                     /locus_tag="PK733_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ11754.1"
                     /translation="MWDNCSFVKDELEAFESYLKDKYKPRNRFLSDVSQKVISSGGKR
                     LRPALVIISGMMGDYDRTKIFPAAAAIETLHTATLIHDDIIDNSKTRRGCPTVSEKHG
                     INLAVYTGDYLLANSILLLSESGLSTDKLDRVAKAARAICTGEVNQYLSRNKLTSVSE
                     YLRRVMRKTGILFSASCTLGAYASGCSEKHVNVMGRIGMNLGIAFQIRDDLIDIESDE
                     TQAGKTVANDIREGIITLPFLFAAKRCEKVRKKIEEVMNGGVEIKEIINDVLTIGGAD
                     DARRLKDRYIEKCRDLSVTLPGGNSTQALDEILCWL"
     gene            complement(3431..5356)
                     /locus_tag="PK733_14340"
     CDS             complement(3431..5356)
                     /locus_tag="PK733_14340"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ11755.1"
                     /translation="MSNKVVIVGAGYAGIEAALTLNKYKKKDKKEKIEVTIIDKNPYH
                     TLLTELHEVAGNRVSEEAVRIPLSEIFKFTDVKVVYDEIEAFDFNNNKVSSTNKEYEY
                     DYLIMAMGSTPNFFGIPGLKENAFTLWSYEDAIRIREHIKKCFRLAVHEEDKQERIKL
                     LTFVVGGAGFTGVEMAGELAHWTKGLAREHGIDYKEVRLLLVDMLPHILNCLSEKNSK
                     KAHDYMEKKLGIEIMLNTPVKEVTSEGISTGNAFINTKTLIWAAGITACEATADMDVE
                     KVGRSRRLKVDEFCRTNHENVYAVGDISNLVDENGVPYPAMVENAIQTAEGAAKNIIR
                     SIKGKELEKVTVKMHGTMVSVGNYFTVSEIMGKILPVWLSIIMKYMVNIHYLWEITGF
                     RGVGRYLYHEVLERRHRKLFLEKHWSTRMQAWWLTLLRVFLGVIWLYEGIKKVSEGWL
                     SSPKLASFLGMAADATSTPTGAGIFVKRIDELFNLDLVVFHFMLGKESRLVEGNIIAS
                     DLFAKLEILHIGSFNPIPWILRNIVLANGTVAMIFQVLVVILEILVGLMLIGGAFTFI
                     ASLISFGLMMMFITSTGIYEKTWWMIFASIAAMGGAGRAFGLDYYLIPYIVNVWDYFW
                     KNRKLRLLFSGGLERFE"
     gene            complement(5476..6408)
                     /locus_tag="PK733_14345"
     CDS             complement(5476..6408)
                     /locus_tag="PK733_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOJ11756.1"
                     /translation="MIKKLETLYKGFWQVADPKIWIASTVPMVLGVTLSAAFGKGFSL
                     FWMILSFVGIYLIEIGKNAINECFDYISGADTAVDSEHRTPFSGGKKTITEGILTLKQ
                     SAIIGATTMFFAAIIGLLIVYFRETSVIYIGIAGFILAAIYSLPPFKLCYRGLGEFAV
                     GIAFGPLLLNGMYVVMAGRYDVLPVLVSLPIGFLITNVLWINQFPDYEADISAGKKNW
                     VVRLGKKESVKVYAIIFILAYIAILAIAVYTLNPVWLIGLLTAPLAAKAVKNCSINFN
                     NITSLIQSNAATVQTYMLNGILMVIAAIIDSLIL"
     gene            complement(6743..7267)
                     /locus_tag="PK733_14350"
     CDS             complement(6743..7267)
                     /locus_tag="PK733_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOJ11757.1"
                     /translation="MKKLVINSILISMALILSYVERMFPIGLIVPIPGIKLGLANVVT
                     IFAIFYLDMKSAIAITVLRCVIASTLFGSIYSLLFSLSGALFALLVMAILKKGYNRWF
                     SILGISIGGAAAHNIGQILIASLIMQSTAIFPYLSILLLAAVITGTLTGLVFSSLMEH
                     IDKINIFKRRIFFL"
     gene            complement(7387..7770)
                     /locus_tag="PK733_14355"
     CDS             complement(7387..7770)
                     /locus_tag="PK733_14355"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOJ11758.1"
                     /translation="MVSIKPRPGDFALLAIILAIAVVNIMMFMQKKDGSPTALIIKDG
                     KTLQSIKLDEVKERIEIKGQGKYNELIAVENGRIRFEESDCPDKTCVRTGWISRSGQS
                     AVCLPAGIIIKIVGDDISDTDIILK"
     gene            complement(7798..9021)
                     /locus_tag="PK733_14360"
     CDS             complement(7798..9021)
                     /locus_tag="PK733_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11759.1"
                     /translation="MEKNLNISKPNLDEWGQLYQASIEFKKIKPWEWMYDDDIFGVKD
                     AESGEIAYCCIMGNEDEYFGIAAYLGSEGLNGILSILSGEIEPDDPDFIFSQKCLLVS
                     FEDREALTSEDRKIIKELGIKFRGRNEWPIFRNYTPGYFPWFITAAECRFLTDILKQA
                     TYVALKCKEEGKDFLETESKCKYIDSEYGHEYEHKNGNRKDDKYDECDTCDECDTCDE
                     CDECDACGDELIFYTRTPRKKDRHIEWNSDYIAPEVHSMEYASFYLTDELKIRKLQNL
                     GRRRGPAWEVDTFYSPGPVQETKGERPYYPKVCLVYDQGRGMILGFDVVKNIREEGSK
                     FLELVSRLIEECKQPPSRILVQREETYYLLFDFCKQVGINLENVYVLENLDEARYEMR
                     NFFDSINETGDEWDD"
BASE COUNT         2823 a         1983 c         1334 g         3032 t
ORIGIN      
        1 acatatggta aagtttcgta ccgaaaccac agctttcatc tgctccatta aaataaagag
       61 gattcccgct gtgtttccta aagtataatg accaatttga gggatgatat atttattaat
      121 tgtactttcc aagcgatcat aaaaggtgtc aaaaaacaga gattttctgt aatattacag
      181 taacaatgag acagctttta aaaagcaaaa agtcctgaaa ccgtttgttt atgaggttcc
      241 aggacttctg ttttggaggc gccacccaga tttgaactgg gggataaagg ttttgcagac
      301 ctctgcctta cctcttggct atggcgccaa aattatttct ttcctgcgta gtatagtata
      361 gcaggtaatg catacctagt caagagtttt tcggaaaatt tcagcaaatt attatccata
      421 tattgttaat tcaaatattc ccccaattat tgacgaatcc ataaattatc catctgctac
      481 ttacctgttg caccacccaa tttcttcatt gatttcttca tgttatctgt aacagcaatg
      541 ctgccatctt gcataatcca taaagcctct acaccatcta agctttctac caactcacga
      601 cttttttcat atgggagtaa aaaaactgtt gtggataaaa aatcagcttg gcccgagtcc
      661 ttcgtcatta ctgttacagc ccgataataa tttgctggca taagagtaga aggttcaatt
      721 agatggtgaa ttcttttgcc gtcaacctca taaaacctct ggtaatcacc actggaaact
      781 actgaagtat cagtgacaaa tacagtatca agagcttcgc cttcaggaat tattgcattt
      841 ccatcagggt tctggatgcc tattccccat ttgcttcgtg tgccatccaa tggcttaccc
      901 actacccgca catttccgcc actgcttata ataaatgaag tgtatccggc ggcctccaat
      961 tctcttgcga caatctctgt agcaaagcct tttgctacag ctcccatatc aagactcatg
     1021 ccggtttctt taagaaatac tgttttttta actgcatcaa cttccacttt tttaatatct
     1081 gtcttcaatt ccgctttttt caataacaca atgtcaggta ttttcgcatt tgcagggtcc
     1141 ctcattcctt cctctctgta tgtgtgccat atggaaagta caggtcccag tgcaatgtta
     1201 acaacaccac ccattttatc gtaccattcc ttactaaatt gaattatatc tataatttct
     1261 tgttttacct caacaggctt tattccagca ttatcattga tggttttaat attgttaatg
     1321 ccatcataaa tattatatat gtcaaaaagc ttatgaagtt cctcaaatct agcctgccct
     1381 tttttagcaa gttcttcaaa ctgcttttca ttttcagcgt aacccataaa ttgtattaca
     1441 gtgtcaaaag taccgataaa ttcatatgta tatttatgat acttaccgcc actaaaccca
     1501 gcccagttgc tgcatccggt actcaacacc aatagaacag tacttgtcag cattaaaaat
     1561 gcaattaatc gaagcattcg ccgtttcaat ataatcaccc cattaaaaag aatcgaaccc
     1621 ctggcatttt aatggtacaa tattttagta ttacacaggg gttctgatat ctttaaaatt
     1681 cccacactat tataatccct atattagctg catttatttt acctgaagaa aatattgatt
     1741 atgttatttt gcatttttaa cagcttcttc gactgcagca atgtaatcct caacagaaag
     1801 ggtaacttta cttgtaagat ccggctcatc gggaacattt gtatgagtgt catcaacctt
     1861 cttaaccttc atagtcttaa tctgatcgat agttttacct accatccatt tttgaagttc
     1921 gtcaatttgc tcataccatt ccttaccaat cttggaaccc ttaatcatac cgtaatcttt
     1981 gccaagttca cgttttgttt tttgttctgc attaagatca gatttaatct ttcctgttgc
     2041 atcaaataat actgtagtct gggcactatc aatagttaca ctgacaattt tgccactgga
     2101 atcaactgat acagcggcca taattgtatc cacctgagca gcgccatctt tatctgcagc
     2161 tgcatctgct gatttactaa ttgatgtaac ctggccaagt ccagttttta ctgcagctga
     2221 agaactgcag ccggtaaaaa gaataatagt aagtgtaaca acagcaaggg aaattgcaat
     2281 gatttttttc atagatgctt ctcctttcgg ttttttgaat aaatattata taaatttcct
     2341 atataatgta aaccctattc gggatcacaa ccaacataaa atttcatcca gggcctgggt
     2401 actattgcca ccaggaagag tcacggaaag atccctgcac ttttctatat aacgatcctt
     2461 cagccgtctt gcgtcatccg caccacctat tgtaagcaca tcatttatta tttcctttat
     2521 ctcaactcca ccattcatga cttcctctat tttctttcgc accttttcac aacgcttggc
     2581 agcaaataaa aacggaagtg taataattcc ttcccggata tcatttgcaa cagttttacc
     2641 agcctgggtt tcatctgact caatatctat taggtcatca cgtatctgaa aggctatacc
     2701 cagattcata ccaatacggc ccataacatt tacatgtttt tcactgcagc ctgatgcata
     2761 ggcaccaagt gtacacgatg cagaaaaaag tattcctgtc tttctcatta cccttcttaa
     2821 atactcacta acagaagtta acttattgcg gcttaaatac tgattaactt cacctgtgca
     2881 gatggctctg gcagcctttg caactctatc aagtttatca gttgataaac ccgactccga
     2941 aagcagcaaa atcgagttgg ccagaagata atcgccggtg taaactgcaa ggtttatacc
     3001 atgcttttcg gaaactgttg gacacccacg tctagttttg gaattatcaa ttatatcatc
     3061 gtgaataagg gttgcagtat gaagagtttc aattgcagct gctgcaggga aaatcttcgt
     3121 cctgtcataa tctcccatca tgcccgaaat aatcacaaga gcaggtctca gacgttttcc
     3181 tccagatgag attacttttt gtgaaacatc acttaagaat ctattcctgg gtttatattt
     3241 atcttttaaa taagactcaa aagcttccaa ttcatcttta acaaagctgc agttatccca
     3301 cataggtttt caactccaaa tctctacata atcaagaaaa ttcgctttgc tcatagccta
     3361 agtattgctg tgaattttct tgttctccag ggaaccccag gttcccttcg actgcttgca
     3421 ccaacccagg ctattcaaat ctttcaaggc ctcctgaaaa tagcagcctt agtttacggt
     3481 ttttccagaa ataatcccac acatttacta tatatggtat tagataatag tccaaaccaa
     3541 atgcccggcc ggctcctccc atagctgcta tagaagcaaa gatcatccac cacgtttttt
     3601 cataaatacc tgttgatgta ataaacatca tcataagtcc aaatgaaatt agcgaagcta
     3661 taaatgtgaa agccccacct ataagcatta aaccaactaa tatttcaagt attacaacta
     3721 atacctgaaa tatcattgca acagtaccat ttgccagtac tatattcctg agtatccacg
     3781 gtatgggatt aaagctgcct atatgcagaa tttccagttt ggcaaataaa tcacttgcga
     3841 taatattgcc ttctacaagc ctgctttctt ttcctagcat aaagtgaaaa acaacaagat
     3901 caaggttgaa cagctcatca attcttttaa caaaaattcc tgctcctgta ggggttgaag
     3961 ttgcatctgc agccattccc aaaaatgaag cgagtttggg tgaagaaagc cagccctctg
     4021 atactttttt tatgccttcg tacaaccata taactcctag aaaaaccctc aacaatgtaa
     4081 gccaccaagc ttgcattctt gtagaccagt gtttttccag gaagagcttg cgatgtctac
     4141 gctcaaggac ttcatgataa aggtatctgc caacccctct gaatcctgta atctcccaca
     4201 agtagtgtat atttaccata tatttcatta taattgaaag ccataccgga agtattttcc
     4261 ccattatttc agatacagta aagtagtttc caacagaaac cattgtacca tgcattttaa
     4321 ccgtaacttt ctcaagttct ttacctttaa tcgaccttat aatgttcttt gctgcccctt
     4381 cagctgtttg aatagcattt tcaaccatag ctgggtaagg tactccattt tcatctacaa
     4441 gattggatat atccccaaca gcgtaaacat tttcatgatt tgtacggcag aactcatcca
     4501 cctttaatct acgtgaacgt ccgacttttt ctacatccat atctgcggtg gcttcacaag
     4561 ctgttattcc ggctgcccag ataagtgttt ttgtattaat aaaagcatta ccggtagaga
     4621 ttccctctga agtaacctct tttacaggag tattaagcat tatttctatt ccaagctttt
     4681 tctccatata atcatgagct tttttactgt ttttttcact aaggcaatta aggatatgtg
     4741 gaagcatatc aactagtaaa agccttactt ctttgtaatc tattccatgt tctcttgcaa
     4801 gacctttagt ccagtgtgcc aattcgccag ccatttctac gccggtaaat cccgcaccac
     4861 ccacaacaaa tgtcaatagt tttattcttt cctgcttatc ttcttcatgg actgcaagcc
     4921 tgaagcattt tttaatatgc tcacgaattc ttattgcatc ttcgtatgac cagagtgtaa
     4981 atgcattttc tttaagtccc ggaattccaa aaaagttagg agtgcttccc attgccatta
     5041 ttagatagtc atactcatat tccttattag ttgatgatac tttgttatta ttaaaatcga
     5101 atgcttctat ttcatcatat acaaccttaa catcagtgaa cttgaatatt tcactaagag
     5161 gtatgcgaac cgcctcttcg gaaacacgat ttccagctac ctcatgaagt tctgtaagta
     5221 aagtatggta aggattttta tcgataatag ttacttctat tttttctttc ttgtctttct
     5281 ttttatattt gttaagtgta agagcagctt cgatacctgc ataccccgca ccaacaatta
     5341 ctaccttgtt tgacatttct tttttcctcc tgttttagtt cttcatagtt aaagtaagct
     5401 tgatttctgc catcaaaatc ctatacaata atttcaaaca taagatcaac gataaaatat
     5461 attataagat ataatctata atattaggct gtcgataatt gcagctataa ccatcaaaat
     5521 tccattaagc atatatgttt gaacagtagc agcattagac tgaatcaaac ttgttatgtt
     5581 gttaaagttt atactgcagt tttttacagc ttttgcagcc agcggagcag ttaataagcc
     5641 aataagccat acaggattca aggtatatac tgcaatagca agaatagcta tataagcaag
     5701 aataaatata attgcatata cttttacaga ttcctttttt ccaagcctca ccacccagtt
     5761 tttctttccg gctgagatat cagcttcata atcaggaaac tgattaatcc atagaacatt
     5821 ggttattaaa aaacctatag gaagtgatac cagcacaggc aaaacatcat atcgtcctgc
     5881 catcacaaca tacatgccat ttaataaaag cggtccaaat gctatgccga ctgcaaattc
     5941 gccaagccct ctatagcaaa gcttgaacgg aggaagacta taaattgccg ctaatataaa
     6001 acctgcaata ccaatgtaaa ttacagaagt ttccctaaaa tatactatta gcaaaccaat
     6061 aatggcagca aaaaacattg tagttgcacc aattattgcc gattgcttta aagtcaggat
     6121 cccttcagta atagtctttt tcccaccgga aaaaggagtt ctatgttcac tatccacagc
     6181 agtgtctgca ccagatatat aatcaaaaca ttcattaatt gcatttttcc ctatttctat
     6241 aaggtaaata ccaacaaacg atagaatcat ccaaaataga ctgaagcctt tgccaaaagc
     6301 tgccgaaagg gttacaccta gtaccatagg aactgtggat gctatccata ttttggggtc
     6361 tgcaacttgc cagaatccct tataaagtgt ttctaatttc ttaatcaaaa aatatcctcc
     6421 tgtctcagtt tgttaatgaa ttaacatcta aaagcacgaa ttcctaaagc ttttgcaata
     6481 ttacttaaat tacaatgtaa tattttatat ttttatacca catataataa aatgataaac
     6541 taaaatgttt tgttttttta agaaatatat accatgagtt attgttgcaa tgtttggaac
     6601 ctttattcgc acttcttgtg ataatctata gataatgtac aaaagaaact acattacaaa
     6661 agttttatac tcttatgtta ccttaatgaa tacgcaaaaa ttcaatttat tccatgtaaa
     6721 ataattttcg cggtatatct attcataaaa agaatattcg tcttttaaaa atattaattt
     6781 tatcaatatg ttccataagg ctactgaaaa ccaacccggt tagtgtaccg gtaattacgg
     6841 ctgccagcag cagtatggat aagtatggaa atattgcagt gctttgcatt ataagcgaag
     6901 caattaaaat ctgacctata ttatgtgcag ctgcacctcc aatgctaatg cctaaaatag
     6961 aaaaccacct gttataccct ttctttaata tagccattac cagcaaagca aataaagccc
     7021 ctgacagtga aaacaataaa gagtatatac taccaaaaag cgttgatgca ataacgcacc
     7081 ttagcacagt tattgcaatt gcagatttca tgtcaagata aaatatggca aaaatagtaa
     7141 caacatttgc caaacccagc tttatacctg gaataggcac tatcaaccct atagggaaca
     7201 ttctttccac atatgaaagt attagcgcca tggatattag tatactatta ataacaagtt
     7261 ttttcattta cccctcattt tataggtgca ttttattact atgattttac tcctataccg
     7321 gagtgaatat tatacttgga aatcatcatt tgctatattt ttcttcaatt ataaaaaatg
     7381 cacctttcat tttaaaatta tatcagtatc agaaatatca tcgcctacaa tttttataat
     7441 aatgcctgca ggtaaacaaa ctgcagattg tcccgacctt gatatccagc ctgtccttac
     7501 acaggtttta tcaggacaat ccgactcttc aaaacgtatt ctaccatttt caactgctat
     7561 caactcatta tatttgcctt gaccttttat ctctatacgt tctttgactt catcaagctt
     7621 tatactctgt aatgttttcc catcttttat gataagtgct gtagggctac catccttttt
     7681 ttgcataaac atcattatgt ttactacagc aatagcaagt atgatggcaa gcaaagcaaa
     7741 atctcccggt cttggtttaa ttgacaccat tgccaccccc tgttgcctat ctgacactta
     7801 atcatcccat tcatcaccag tttcatttat tgaatcgaaa aaattacgca tttcatacct
     7861 tgcttcatct aaattctcta aaacatacac attttccaag tttataccta cctgcttgca
     7921 aaaatcaaat aacaaatagt atgtctcctc tctttgtaca agtattctgg atggcggttg
     7981 cttgcattct tcaattagtc tggaaaccaa ttcaagaaac tttgaacctt cttcccggat
     8041 attctttact acatcaaagc caagaatcat tcctcttccc tgatcataga caagacacac
     8101 cttgggataa tagggacgtt cacctttagt ttcctgcaca ggcccaggac tgtaaaaagt
     8161 atcaacctcc caggcaggac cccttcttcg acccagattt tgtagctttc ttattttcag
     8221 ttcatctgtt aaatagaaag atgcatattc catggaatgt acttcaggtg ctatataatc
     8281 actattccac tcaatatgtc tatctttttt tcggggtgtg cgagtataga atataagttc
     8341 atcgccacat gcatcacatt catcacattc atcacatgta tcacattcat cacatgtatc
     8401 acattcatca tatttatcat cttttctatt tccattttta tgctcatatt catgtccata
     8461 ttcagaatca atatatttgc atttactttc agtttcaagg aaatccttgc cttcctcttt
     8521 gcattttaga gcaacatatg ttgcctgttt tagaatatca gttaggaatc tgcattcagc
     8581 agctgtaata aaccatggga aataccctgg tgtatagttc ctgaatatag gccattcatt
     8641 ccttccacgg aattttatac ccagttcctt gattattttt cggtcctcac tagttaacgc
     8701 ttctctatct tcaaaggaaa ccaataaaca tttttgagaa aatatgaaat ccgggtcgtc
     8761 aggctctatc tcccctgaaa gtatacttag tataccattg agaccttctg aaccaagata
     8821 ggcagcaata ccaaaatatt catcctcgtt tcccattatg caacagtatg caatttcacc
     8881 gctttcagca tcttttacac caaatatatc atcatcatac atccattccc atggcttaat
     8941 tttcttaaac tcaatagaag cctgatataa ctgaccccat tcatcaaggt tgggtttgga
     9001 tatatttaaa tttttttcca taataatacc tctcaatcat tgtttactaa aatatcattt
     9061 tttaaattat atttctttaa tattagtagt gtaccataat agactatttg taatcaacaa
     9121 attaagcaga aatggggtgc attttaattt tgaaaaaata tattccagaa gc
//
LOCUS       DAOSDD010000185         9135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2823_length_9135_cov_5.121256, whole genome shotgun sequence.
ACCESSION   DAOSDD010000185 DAOSDD010000000
VERSION     DAOSDD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2823_length_9135_cov_5.121256"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK733_14365"
     CDS             <1..884
                     /locus_tag="PK733_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ11760.1"
                     /translation="GLFMKIGVSSYSFSGLMASGAMTQLDIIKKAKEMGFDVIEFSGL
                     KVPEGETAEDFAVKVKEECARVGIEMGNYTIGADFINGSDGNLDAEIERLKGQVRIAK
                     ILGSRGMRHDATSGFKPGYEGPKGFDDALPILIKGCRAVTEYAESLGIRTMMENHGFF
                     AQDSERVEKLANGVNHKNFGLLVDIGNFMCVDEDPAKAVGRVAPYAFHVHAKDFHVKP
                     GTASNPGTGWFASRAGNYLRGAIIGHGEVPVVQCLRILKKAGYNGTVSIEFEGMEEPL
                     KGIAVGLENLRKYIEII"
     gene            1092..2318
                     /locus_tag="PK733_14370"
     CDS             1092..2318
                     /locus_tag="PK733_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ11761.1"
                     /translation="MELQKSLDYVEKNIQEIIDLTIEICKIPSPTGNEQQKALFIKDK
                     LLEYGANDANIDQAGNVLYLHKNYLSDRRKITLITAHIDTVFHNTENIEPIVKEGKIF
                     APSAGDNSVNVAALIFIIKMLNELEISFKKDILFAFDVGEEGLGNLKGIKAVFDNWED
                     DISEVIALDLAYDNVVNNAVGSKRYMVSVNTEGGHSWGSFGNDNAIFYSSSIIKDLYD
                     IDVPKKPKTTYNVGVIKGGTSVNTIAAYTEFLVDMRSEDKEQLKELDNKFLSIANRYL
                     EEYKGRVAINLQIIGERPCGIKIEARKTKCNTEDSLEFNAEHDSEFNAEHNSECNLEY
                     NSMEERLIRVRGKLNFPVNFVSASTDANIPLSKGIPALSFGVCRGGNSHRLDEYIELD
                     SLDRGMKHLIHFLFDE"
     gene            2680..5004
                     /locus_tag="PK733_14375"
     CDS             2680..5004
                     /locus_tag="PK733_14375"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HOJ11762.1"
                     /translation="MITKIRKRDGREVTFNIDKIANAIFKAAAAAGGKDYDTALKLAE
                     QVAEYLDKNFKNTIPGVEDIQDVVEKILIENGHARTAKEYIIYRAERTRIREMNTRLM
                     KVYEDLTFKDAIDSDLKRENANIDGDTAMGTMLKYGSEGAKQFYELYVLDPSHSKAHK
                     EGDIHIHDLDFLTLTTTCCQIDIKKLFEGGFCTGHGYLREPKDIQSYAALACIAIQSN
                     QNDQHGGQSVPNFDYGMADGVAKTYAKIYRQNLINSLELITGGQEYEESVRQMFAEIE
                     DEHGMKPSLNGSDLYKKLEAKYLDDIIKDKDITNRVQDFTVRKALIETERRTYQAMEA
                     LIHNLNTMHSRAGAQTPFSSINYGMDTSPEGRMVMKNILLATEAGLGNGETPIFPIQI
                     FRVKEGINYNPEDPNYDLFKLACRVSAKRLFPNFAFVDAPYNLKYYKPGHPETEVAYM
                     GCRTRVVGNVYDPEKEIIYGRGNLSFTTVNLPRIALKSGKNVDRFFDELDRIIDLVIN
                     QLLERFEIQAGKKVKNYPFLLGQGIWLDSDKLGKDDEVREVLKHGTLSMGFIGLAECL
                     KALTGKHHGESEGSQNLGLEIIGYMRKRMDETSKKYNMNFTLLATPAEGTAGRLVKID
                     RKLFGSIDGVTDREYYTNSFHIPVYYDISAYDKIRLEAPYHELTNAGHISYVEVDGDP
                     TNNLEAFEKIVRAMKEAGIGYGSINHPVDRDPVCGYTGIIGNTCPKCGREEGNIKFQR
                     IRRITGYLVGTLDNFNDAKKAEERDRVKHFTCKN"
     gene            5106..5615
                     /gene="nrdG"
                     /locus_tag="PK733_14380"
     CDS             5106..5615
                     /gene="nrdG"
                     /locus_tag="PK733_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOJ11763.1"
                     /translation="MKSTLRLAGIVKESIVDGPGIRLVIFAQGCAHNCPGCQNPQTHS
                     FDEGIVVNIDDILAMIKCNPLLDGITLSGGEPFEQAEAFAALAQKVKESGLNIMTYTG
                     YTFEEILNQSDSRTGWKKLLEVSDILVDGRFEIGKKNILLKFRGSENQRIIDVKKTLK
                     TNKIVLTAI"
     gene            5839..7164
                     /locus_tag="PK733_14385"
     CDS             5839..7164
                     /locus_tag="PK733_14385"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ11764.1"
                     /translation="MELKMLKCGLAEVDITPILGSIIPGQFHVRYSTGVKDNLFAKAV
                     VIGNGENTIAIVAMDALFVDSSSVAGIRERIKRTVGIPEENIMVSATHTHTGGPVCSW
                     GDYIRKDPVYIDFIIDKCADAAIMAYKKMKPAVISFGKGCESGISFNRRFLMKDGTVK
                     TNAGIGNPDIVKPSGPIDPDVTIIRVDDENGNIIGVITNFACHLDVVGGDEYSGDYPG
                     ELSRILKKAYGDHVVSLFLTGTCGNINHVDASGRFAIQKEHYIKMGRILAGEVVKTLE
                     RTEVCSDVKLEAGSRLFELGVRQPSAEDLLKAKELLDNTKVDNSELIKETRSQLVETF
                     YAKEAIRMHEEKEKSVYVEVQVLKIGDLAISALPGEIFVEFGLDIKKRSPFKYNMANS
                     LANGCFGYIPVREAFSQGGYEPRLCATSRLEEEAGYKMADAAVELLEKL"
     gene            7294..7369
                     /locus_tag="PK733_14390"
     tRNA            7294..7369
                     /locus_tag="PK733_14390"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7327..7329,aa:Lys,seq:ctt)
     gene            7548..9011
                     /locus_tag="PK733_14395"
     CDS             7548..9011
                     /locus_tag="PK733_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ11765.1"
                     /translation="MVNFLDTIKTNTLIFDGSKGYMLQKFGMKGGECPELWNVEHSDI
                     VKKIYSLYKDAGSDVIQTNTFTGSRVHLEKHGLGHRTYELNYEGTRLAREVMGNDGFV
                     AASIGPTGKLFAPLGDLTFNEAYELFREQVKAVVDSGADIINFETFTDVAEVRVALLA
                     AKDITNLPVICSISFEANGRTLMGTDPETAVLILKSLGADMLGTNCSFGPEFMLDIVK
                     KMYQAGAGYISVKPNAGLPEILDGEPVYKATAERFAGLAADFAKYGARLIGGCCGTTP
                     EFVSAIKKKIDSINLNANLERVCTDKCTDKSTEKMVSSKMISSGVRCIAVGEAGIEKI
                     IFEDMKKSMNIGKLSSYEDKELLSCLKSGDVSIVEDVVMDLAGEDYDAIHICVDNVIE
                     AIIQSYEKADNEECGECTKTGHTLLAQVVNEAQGYLKEPFVIETKYPNALESALRIYK
                     GRAGVILNETLDINDNIKREISNIAKKYGALVIKRNS"
BASE COUNT         3162 a         1373 c         2014 g         2586 t
ORIGIN      
        1 gaggattatt tatgaagatc ggagttagct catatagttt tagcggactt atggccagtg
       61 gtgccatgac ccagcttgat ataataaaaa aggcaaaaga aatgggcttc gatgttattg
      121 agttttcagg acttaaggtt cctgaaggtg aaactgcaga ggattttgct gtaaaggtga
      181 aagaagaatg cgcccgtgta ggaattgaaa tgggtaatta taccattggt gcagatttta
      241 tcaatggttc tgatggaaat cttgatgcgg aaattgaaag gcttaaagga caggtaagaa
      301 ttgcaaagat attgggttca agaggaatgc gccatgatgc taccagcgga tttaaacctg
      361 gttatgaagg tccaaaagga tttgatgatg ctttacctat tttaataaaa ggctgcagag
      421 cagtaacaga atatgctgaa agccttggta tacgtaccat gatggaaaat catggctttt
      481 ttgcccagga tagtgagaga gtcgaaaaac tggccaatgg ggttaaccat aaaaactttg
      541 gccttctggt tgatattggt aatttcatgt gtgttgatga agaccctgca aaagcagtag
      601 gaagggttgc accctatgct ttccatgtgc atgccaaaga cttccatgta aaacctggca
      661 ctgcttccaa tcctggtaca ggctggtttg cttcccgtgc cggaaattat ttgagaggcg
      721 caataatcgg tcatggggaa gttcctgtgg tccagtgcct tagaattctt aagaaggctg
      781 gttataatgg cacagtttca attgagtttg aaggtatgga agaacccctt aaaggtatag
      841 ctgttggcct tgaaaatctg agaaagtata tagaaattat ttaggaggaa agcaagagat
      901 gaaagaggga agattctaat tgcacaagaa atcaaacaat aaataaagtg caagagggac
      961 ggttctattt tgccgggtaa gaaagggaca actttatttt actctaataa ttaaagtctg
     1021 tccctttatg caaattttgt ctctttacaa agatcccttg aaatagggat tctcttgttg
     1081 cttaattcat tatggaactt caaaaatcac ttgattatgt tgaaaaaaac atacaggaaa
     1141 taatagattt gactattgaa atttgtaaga taccatctcc aaccggaaat gaacagcaaa
     1201 aggctttatt tatcaaagac aagcttctgg aatacggagc aaatgatgct aatatagacc
     1261 aggctggaaa tgttctttat ctacataaaa attatttatc tgatagacgt aaaataactc
     1321 tcattacagc tcatattgat acagttttcc acaatactga aaatattgag ccaattgtta
     1381 aagaaggaaa aattttcgct ccttcggcag gggataatag tgttaatgta gcagctctta
     1441 tttttataat aaaaatgctc aatgaactcg agatatcttt taaaaaggat atcctttttg
     1501 cctttgatgt tggagaagaa ggacttggta atttgaaagg aatcaaggct gtttttgata
     1561 actgggagga tgatattagt gaagtaattg cactggacct tgcatatgac aatgtagtaa
     1621 ataatgcagt aggttcaaaa agatatatgg tttctgtcaa taccgaagga ggccatagtt
     1681 ggggcagctt cggaaatgat aatgcaattt tttattcatc aagtataata aaggatttat
     1741 atgatattga tgtccccaaa aaacctaaaa caacttataa tgttggggta attaagggtg
     1801 ggacatcggt aaatacaatt gcagcatata cagaattttt agttgatatg cgttcagaag
     1861 acaaggaaca gctaaaagaa cttgataata agtttctttc tatagcaaac aggtatttag
     1921 aggaatataa gggtagagtt gcaataaacc ttcaaataat aggtgaaaga ccatgtggta
     1981 ttaagattga agctagaaag accaaatgca atacagaaga tagcttagag tttaatgcag
     2041 aacatgattc agaatttaat gcagaacata actcagaatg taacttagaa tataattcta
     2101 tggaagaacg tttgataagg gttcgaggca aacttaactt tcctgtgaat tttgtttcag
     2161 caagtacaga tgccaatatt ccattaagca aaggcatacc cgccctttct ttcggcgtat
     2221 gcagaggagg aaacagccac agacttgatg aatatattga attggattca ttggatagag
     2281 gcatgaaaca tctcattcat tttttatttg atgaataata gcataggaac tgtccaaatg
     2341 actcgttcac aagccaataa gtaataaatg aggagtcaat gatgacagtt cttattttat
     2401 tgaccttttt acttaaattt ttaaatccta tgaattttgg agtatggcta tcattttctt
     2461 atattttatt ctgtacatcc ctaaaattga gtaaaaacga aattgactca atatattgtg
     2521 gtctgatatt atactcacat caacatgtag ataaatactt tattaatttc tctaaaaatg
     2581 aaattacact attctacatt acactacact acactacact acaagataat ttttgttttg
     2641 aaaatatatt tgttaacact tatatgggag gttttttact tgataacaaa gatcagaaaa
     2701 cgggatggca gagaagtgac ttttaatata gacaaaatag ccaatgctat atttaaggcc
     2761 gctgcagcag caggaggaaa ggattatgat actgcactaa aattggctga acaggttgca
     2821 gaatatcttg ataaaaactt taaaaatact ataccgggtg ttgaggatat ccaggatgta
     2881 gttgagaaga ttctcattga aaacggccat gcacgtacag ctaaggagta cataatatac
     2941 agagccgaaa gaacccgtat aagagaaatg aatacacggc taatgaaggt ctatgaagac
     3001 ctgaccttta aagatgccat agatagcgat ttaaagcggg aaaatgcaaa tattgatgga
     3061 gatactgcca tgggtaccat gctcaaatat ggttctgagg gtgcaaaaca attctatgaa
     3121 ttatacgtat tagatccatc ccactccaaa gctcataagg aaggtgacat acatattcat
     3181 gatctggatt ttctgacact tactaccaca tgctgccaaa ttgatattaa aaagcttttt
     3241 gaaggcggat tttgtacagg acacggttac ctgagagagc caaaggatat tcaaagttat
     3301 gcggctcttg catgcattgc aattcaatct aatcagaatg accagcatgg tggtcagagt
     3361 gttccaaatt ttgactatgg tatggcagat ggggtagcaa agacttatgc caagatatat
     3421 cgacagaatc ttattaactc ccttgagctt attacaggcg gacaggagta tgaagaaagt
     3481 gtaagacaga tgtttgcaga aattgaggat gagcatggga tgaagccttc cttaaatggc
     3541 agcgatcttt acaaaaaact ggaggctaaa tatctagatg atattattaa ggataaggat
     3601 attaccaata gggttcagga ttttacagtc cggaaagctc tgattgaaac tgaaagacgt
     3661 acatatcagg caatggaggc gctcatacat aaccttaata caatgcacag ccgtgcaggc
     3721 gcacagacac cattcagttc catcaattat ggaatggata catctcctga aggacgtatg
     3781 gtaatgaaaa atatccttct tgctactgaa gcaggtctag gaaatgggga aacaccaatt
     3841 ttccctattc aaatattcag agtcaaagaa ggaataaatt acaacccgga ggatcccaat
     3901 tacgatttgt ttaagctagc atgcagagtt agtgcaaaga ggcttttccc aaactttgca
     3961 tttgttgacg ctccgtacaa cttaaaatat tacaaacccg gacatcctga aactgaagtt
     4021 gcatatatgg gttgcaggac tagagttgtg ggcaatgtat acgatcctga aaaggaaatt
     4081 atttatggta gaggtaattt aagctttaca actgttaacc ttccgagaat agcattaaag
     4141 agcggtaaaa atgtagatcg tttctttgat gagctggata gaataattga ccttgtcatc
     4201 aatcaactac ttgaaagatt tgaaatacaa gccgggaaaa aagtgaaaaa ttatcctttc
     4261 cttttgggtc agggtatatg gcttgattct gataagcttg gtaaagatga tgaagtgagg
     4321 gaagtattga agcacggaac attgagcatg ggatttattg gcctggcaga atgccttaag
     4381 gctttaacgg ggaagcatca tggggagtct gaaggatccc agaaccttgg ccttgaaata
     4441 ataggctata tgcgtaaacg tatggatgaa acaagcaaaa aatataatat gaattttacc
     4501 cttcttgcca caccggcgga aggaacggca ggacgacttg taaaaataga cagaaaacta
     4561 tttgggagta ttgatggcgt aactgataga gaatattata caaatagctt tcatatacct
     4621 gtttattatg atatcagtgc ttatgataaa ataaggcttg aggctccata tcatgagtta
     4681 accaatgcag gacacatatc ttacgtagag gttgatggtg atcctacaaa caatcttgaa
     4741 gcttttgaaa aaatagtaag agcaatgaaa gaagcgggaa taggctatgg ttccattaac
     4801 catcctgtag acagagaccc tgtttgcgga tatacaggaa tcatagggaa tacatgcccg
     4861 aaatgtggaa gagaagaagg aaacattaag ttccaaagaa tacgtcgcat tactggctat
     4921 ctggttggta cattggataa tttcaatgat gccaaaaaag ctgaagaacg ggacagggta
     4981 aagcatttta cctgtaaaaa ctaaacaatc aaaaggggac aatcaaaagg ggacactcct
     5041 taacgtaaaa gaaagacttc ttgctcgaga gaggagtgtc cccttacctt aaaggagaat
     5101 catatatgaa atcaactctt agactggcag gaatagttaa agaatccatt gtggacggtc
     5161 caggtataag acttgtcatt tttgcccaag gatgtgcaca taattgcccc ggatgccaaa
     5221 atcctcaaac tcattctttt gatgaaggta tagtcgtgaa tatagatgat attttagcaa
     5281 tgataaagtg caatccgctt cttgatggta tcactttgag tggcggtgaa ccatttgaac
     5341 aggcggaagc atttgctgct ttagcccaaa aagtgaaaga atcagggtta aatattatga
     5401 cctacacagg ttataccttt gaagaaatat tgaaccaatc tgatagcaga accgggtgga
     5461 agaaactttt ggaggtctct gatatattgg ttgacggaag gtttgagatt ggcaaaaaaa
     5521 acatactgct gaaattccgg ggctcggaaa atcaaagaat tatcgatgta aaaaaaacac
     5581 tgaaaaccaa taaaattgta cttactgcta tttaggaaga gaaaaactaa atttgtgcaa
     5641 aataaagtga aaatctgata tatacattta tccaatttta tggtaataat atattaatat
     5701 tgattataat caagtctgat aacacgagtc attatagtag aatacatatt gataaatcta
     5761 tattaactat tattaactat ttaataactg ttaaagcata tgaagttttg tacaaaatta
     5821 ttgggagaag gtgcaggaat ggagcttaaa atgttaaaat gcggattagc tgaggtggat
     5881 attactccta tactaggaag tattattcct ggtcagtttc atgtaagata ttcaaccggg
     5941 gtaaaggata atcttttcgc aaaggcagtt gtgattggta atggagaaaa tactattgca
     6001 atagttgcca tggacgcact atttgttgat agttcaagtg ttgcaggaat aagggagaga
     6061 attaaaagga ctgtgggtat accggaagaa aatataatgg tttcagctac tcatacccat
     6121 acagggggtc ctgtatgcag ctggggcgac tatataagaa aagacccggt atatattgat
     6181 tttataattg ataaatgtgc tgatgctgca attatggcct ataaaaaaat gaaacctgct
     6241 gtgataagct ttggtaaagg ttgtgaatct gggatatcat ttaacagaag attcttaatg
     6301 aaggatggaa cagtaaaaac taatgccggg attgggaatc ctgatattgt caagccatca
     6361 ggcccaatag atcctgatgt tactataata agagttgatg atgaaaatgg caatataatt
     6421 ggggtaataa ccaattttgc ctgccatctg gatgtcgtcg gtggtgatga atacagcggg
     6481 gattatccgg gagaactgag caggatacta aaaaaagctt acggtgacca tgtagtaagt
     6541 ctatttctta cagggacttg cggcaatata aatcatgtag atgcaagtgg cagatttgct
     6601 attcaaaaag agcattacat aaaaatgggt agaatattgg ctggagaagt tgtaaagact
     6661 cttgaaagga cagaggtgtg cagtgatgta aagctggaag ccgggtcacg gttgtttgag
     6721 cttggagtca gacagccttc cgctgaagat ttattaaaag ccaaagaact tctagacaat
     6781 acaaaagtag ataacagtga actgataaag gaaaccagaa gtcaattggt tgagaccttc
     6841 tatgccaaag aagccatacg tatgcatgaa gaaaaagaaa aaagtgtata cgtagaggtt
     6901 caggtgctaa aaataggaga ccttgccatt tccgcactgc caggcgagat atttgttgag
     6961 tttgggttgg atattaagaa aagatctcca ttcaaatata atatggcaaa ctcccttgca
     7021 aatggatgtt ttgggtatat acccgtacgg gaagcctttt cacagggagg ttatgagcca
     7081 agactttgtg ccaccagcag gcttgaggaa gaagcgggtt acaaaatggc cgatgctgct
     7141 gtggagttat tggagaagct ataaaaagtg ctattaaaaa tgtgcaaaaa aaatttagct
     7201 tttattgttg acaacatggg taattacctt tataatatta attgttcgaa aaaagcggtt
     7261 gaacaaacac caattgttta gccgctaaat tatgggccat tagctcagat ggtagagcac
     7321 ctgactctta atcagggtgt cccgggttcg agtccctgat ggcccaccac acataagccg
     7381 cttatcacaa taggtaagcg gcttatgcta tataacgtga aaatcaaata aacggttgaa
     7441 agtgctaaaa aattgtgaaa agtcttataa aagttgtgaa aaagctgtaa aggggctatg
     7501 atggaaagtt atattataca tctagattat atagggagct gataaaattg gtaaatttct
     7561 tagacacaat taaaactaac acactcatat ttgatggttc aaagggatat atgcttcaga
     7621 aattcggcat gaaaggcgga gaatgccctg aattatggaa tgtggaacat agtgatatag
     7681 taaaaaaaat atatagttta tataaagatg caggttcgga tgtaattcaa accaatactt
     7741 ttaccggcag cagagttcac cttgaaaagc atggcctggg acatagaaca tacgaattaa
     7801 actatgaagg taccagactg gcaagggagg taatgggaaa tgatggtttt gtagctgcat
     7861 caataggccc gacaggtaaa ctatttgcgc ctttgggaga tcttaccttt aatgaggctt
     7921 atgaactgtt cagggagcaa gttaaagctg tagtagatag cggagcagat attatcaatt
     7981 ttgagacatt tactgatgta gccgaggtga gggttgcttt gcttgctgca aaagatatta
     8041 ccaatcttcc tgtaatctgc tcaatatcat ttgaagcaaa tgggagaact ttaatgggta
     8101 cagatcctga aactgctgtt ttaatattaa aatcactagg cgctgacatg ttgggtacaa
     8161 attgttcttt cggtcctgag ttcatgttag atattgttaa aaaaatgtac caggcaggag
     8221 cggggtacat tagtgtaaaa cctaatgcag gtttgcctga aatcctagat ggtgaacctg
     8281 tttataaagc aactgccgaa agatttgctg gcttggcggc tgattttgca aaatatgggg
     8341 caaggctgat aggaggctgt tgcggtacta ctccggaatt tgtaagtgct ataaagaaaa
     8401 aaattgacag tattaatttg aatgccaatt tggagagagt atgcactgat aaatgcaccg
     8461 ataaatctac cgaaaaaatg gtttcatcca aaatgatttc ctccggcgta agatgtattg
     8521 cggttggtga agctggaatt gaaaagatta tttttgaaga tatgaagaaa agtatgaata
     8581 taggtaaatt aagttcctat gaagataaag aactattatc ctgccttaaa tccggagacg
     8641 tgagtattgt agaagatgta gtaatggatc ttgcaggaga agattatgac gcaatacata
     8701 tatgcgttga taatgttata gaagctataa tacaaagcta tgagaaagct gataatgaag
     8761 agtgtggaga gtgcacaaaa accggtcata ctcttttggc ccaagttgta aatgaagccc
     8821 aaggttattt aaaggaacct tttgttattg aaaccaaata tcctaacgca ctggagagtg
     8881 ccttgagaat atataaaggg agagcaggag ttattcttaa tgagacttta gatattaatg
     8941 ataatattaa acgtgagatt tcgaatatag ctaaaaaata tggcgcactg gttataaagc
     9001 gtaatagctg aaaactttgt aggggtgcat ttaaaatttt tgaaaaaata tgttccagaa
     9061 gctatcgtta cggtacttag agcctttaaa ttcagctacg ggcaaaatca aactcacaaa
     9121 ataatatgaa taaaa
//
LOCUS       DAOSDD010000186         9052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2859_length_9052_cov_4.474380, whole genome shotgun sequence.
ACCESSION   DAOSDD010000186 DAOSDD010000000
VERSION     DAOSDD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2859_length_9052_cov_4.474380"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK733_14400"
     CDS             complement(<1..282)
                     /locus_tag="PK733_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11766.1"
                     /translation="MFDPAIVDLLILRNVDNNRPTEYVKHATLGNMPLSTYGDGIKKV
                     LSLANGIAQAAGGVLLIDEVETAIHAKYYDDIFRKHSGNPLYFNRADESC"
     gene            complement(364..780)
                     /locus_tag="PK733_14405"
     CDS             complement(364..780)
                     /locus_tag="PK733_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOJ11767.1"
                     /translation="MVNIEVIKQRLNQLSISLNKIERFKEISLDEFLKDDIIQDVIEY
                     NLFIAINMMIDIATHIVVDNNIGNPDTLGEAFNILNKEKYLSDNETKVYKNMVGLRNI
                     LSHEYLKINKRIIYNILQNNLVDIKKFIIFIHDNFI"
     gene            complement(773..1180)
                     /locus_tag="PK733_14410"
     CDS             complement(773..1180)
                     /locus_tag="PK733_14410"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ11768.1"
                     /translation="MDNIEKCKNILIGYENIVFAYIFGSYAQRVIRTDSDIDIAIYLE
                     KELAVENYLEIKVDLTEACKREIDLIILNDATPLLKYEIYKNSILLFSRDKAIETSYK
                     VKTLFEYNDMKKYLDLYYDRTINRLKDEVKFYG"
     gene            complement(1573..2322)
                     /locus_tag="PK733_14415"
     CDS             complement(1573..2322)
                     /locus_tag="PK733_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11769.1"
                     /translation="MGDILPVKLVGITRLSFHIMHWYCDYRGLDNMYMDLYDEPKMVH
                     DVMRFFTDGIKHMLQQYENQKLLSLNNDFTFHYTGGIGYTDELPHEGFTPDCVRLRDL
                     WAAAEAQEFSNVSPAMHEEFVLQYERELLENFGLNGYGCCDDPGKKLDGVLKIKNLRR
                     VAVCPWADISNFVPRLGKDYIMTWKPQPSFLAFDYFDTDAVEQELVTGLQKAKGGIIE
                     LILRDTHTCRKDSARFTEWIEIARKAIDNIW"
     gene            complement(2398..2826)
                     /locus_tag="PK733_14420"
     CDS             complement(2398..2826)
                     /locus_tag="PK733_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11770.1"
                     /translation="MSDYMPDKNLIHDKSILRELAKQVAEIAALPEQEERKKLWTSHN
                     TPRAERPVVFVHPDGAWNELLPYESLVCETPLLREIEYDLKQRLIRHKYIPDDVPCHD
                     DINVQKVIHNSMWGVEPKRINSKQAGGTWHHEPIINEPSD"
     gene            2826..3389
                     /locus_tag="PK733_14425"
     CDS             2826..3389
                     /locus_tag="PK733_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11771.1"
                     /translation="MYICSPTSYPTTDKSNSNKQIYFPVRGKFEQQQFKPLIDQLDYL
                     SKRKPFNILRTQAIFLNILDKLASLTKSSASETRELAIQIMDFIAENFNKPLTWNHLS
                     KKFNYSPDYLARIMKKHYGITLGQYIQEFRINKAKELLANTDYPLSAISREVGYSDVS
                     LLYKAFRNQEGIAPGIWRTNCRGVLKH"
     gene            complement(3439..5571)
                     /locus_tag="PK733_14430"
     CDS             complement(3439..5571)
                     /locus_tag="PK733_14430"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ11772.1"
                     /translation="MLIERYGNKISNILLPVDGIKPFPTIDDRARWKLVMPVLSKPVI
                     NEAEVYRNFKWPSLSASDFIESCKKNEDTPERLEVSAKRQVLRTLILAECLNGKNEYI
                     DNIINGIWSICEESSWAIPANSYMHRNWPDPLPDATDPVIDLSSAATASLLTWAYYLM
                     GSAFDKVSVNMNRRIKYEINKRILTPFLENDDIWWMGFQRMMNPRMKVLHPINNWNPT
                     CNSCCLIAFLMLEDDWKRRIQGVLKAMEITDIYIGSYPADGGCDEGISYWSMAPAALF
                     DILEQLYFATDGIIDIYNEPLIKDMGRYFYRAYIGKDNFINFADASSKLRLSNDLVYR
                     YGRRISDDTLADIGAKMFMKEAEAFLREDEGGKLATRKPNILIRELPMLFNNKWMTVL
                     LSKSDSSKSDMTATKCSAEFSCCKIPEGRKVDYYIRDVWLKDIQVMAAREQHGSYEGF
                     YIAAKGGHNAESHNHNDVGNFIVYYNAEPVIVDPGVEIYSGKTFSQERYSLWTMQSSF
                     HNLPSVNGYQQKEGRVYKATNVIYHNDNRKAVLAMNIDKAYPEESGICYWKRSCHLSR
                     DSEKYIEIIEDFKLFELSDDIKLTYMTPCKLVYKSQENSGNETKGKICLKTKEKFEND
                     LKKGIEMDSAKEEPENIYEKEFSKNMSEGRIPFLQFDTDVFDYSYETIQLNDMRLIDQ
                     WGSYLYRIILKPKQKVKEGNWSIKIVAM"
     gene            complement(5774..8989)
                     /locus_tag="PK733_14435"
     CDS             complement(5774..8989)
                     /locus_tag="PK733_14435"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ11773.1"
                     /translation="MNESVENAEYYLSLGIDYLWSLVTSQSIPRSAAVCDDGCPVCRK
                     GINKFGHYPWVADSVNMPWKLECPNCGTFFPSNDFEAYYKSGIGKYGLFYPEIADISL
                     LKNTLYPDKPENWCVDDGYGVIDEKSRRYTFAAYYNHWYIWMDGGGASGERKNSILCI
                     IEALKDAYLYTGEARYGRAGIAILDRVADVYPDMDASQYRIEDGYRHNHGITKKGNIT
                     GAISECEVAKKLISAYDAFFPEIYNKDCTDVISFLNEKAELYGFDNKKTDIDHIRENI
                     ENNIIRLVYPRIKDARIKGNVGMHQSTLAMAALVLEDKNAAEEWMKWLLKPGGLVGEP
                     DWKVTGGNILNVLYYDVDRDGHGNEAAPGYNYLWLSQLKDLADILELYEEYYEKYYKE
                     QDEEFTKQNKARNVINLYKSPKFRKMYLALHPLITLDKYIPSIADSGSSGKPFLYGNK
                     SITLDGFRRYKDPELAQQAYLLNQNKADGLCLNIFHPLAGEIENDVKSVIENKGKLLV
                     ESIDLTGYGYAVLKDGSDNTEIAPGIEEDTRKAAWIYYGRTIWHGHQDALNLGIYAFG
                     LDLTPDLGYPEFARHDCSHRTEWGNNTISHNTVVVDAKKQDRMYTGISKHFEDTLDVK
                     IIDVEAPFAYSEIELYRRSIMMIRVDCENSYIVDIFRVKGGNDHYYSFHGAEANVSSE
                     GIEFKKQDGGTYAGPEVEYMERYDDLKNCTATYKGSGFHYLYNVERSYNPEPGFSIDW
                     DIKDTWGALEKDTDIHLRLTMLNRVNSVALSDGQPPQNKVGNPEKLRYMIAHRSKNND
                     LESQFVSVIEPYKEKRFIKSLEKVSIHILGKITAEKNDKNKETIDTAAIKLVLNNGRT
                     DYIISTLNPETSYLVDNRIIFTGVYGVYSEQGGETAYAFICHGTYMSVDGSVIFNSKY
                     GALTGTLEDFTRCPSFSNRLIVRINEDFGDSDKDDSGNAEDSGKEDFIDHNNLGMIIH
                     KLKGRYIYIENYAARRTGYVMKSIPELREETVQYADIRNAVYRIISAEKIGNNLFALD
                     IGDVTLIRGLADEDDEGKGFLYDAEPGDKFVIPLSQSLKPT"
BASE COUNT         2689 a         1896 c         1250 g         3217 t
ORIGIN      
        1 acagctttca tctgctctat taaaatagag aggattcccg ctgtgtttcc taaaaatatc
       61 atcgtagtat ttcgcatgaa ttgcagtttc aacctcatca attaacaata ctccacctgc
      121 tgcttgcgca attccatttg ctagcgataa tacttttttg atgccatcgc cataagtaga
      181 aagaggcata tttccaagtg ttgcatgctt tacatattca gttggacggt tattgtccac
      241 atttcttaat attaagagat caacaattgc aggatcaaac aactgtataa attcaaatag
      301 aattgtcccc agtgcacctt accaaaatta ccctcaacga gaaggaactc aatctaatta
      361 tatttaaata aaattatcat ggataaaaat aatgaatttt tttatatcga ctaaattgtt
      421 ctgtagaata ttataaatta ttcttttatt tattttgaga tattcgtgag ataatatatt
      481 tctaagtcct accatatttt tgtaaacttt tgtttcatta tcacttaaat atttttcctt
      541 atttaaaata ttgaatgctt ctcctagagt atccggatta cctatattgt tatctactac
      601 tatatgggta gcaatatcaa tcatcatgtt tatagcaata aataaattat attctattac
      661 atcttgtatg atatcatctt tcaaaaactc atccaaagat atttccttga atctttcaat
      721 tttatttaaa gaaatagata attgattaag tctctgttta attacttcta tattaaccat
      781 aaaattttac ctcatctttt agtctattaa tggttctatc ataatataaa tccagatatt
      841 ttttcatatc attgtactca aacaacgttt ttactttata acttgtttct attgctttat
      901 ctcgtgaaaa caacaatatg ctattcttgt aaatttcata ttttaataaa ggggttgcat
      961 catttagtat aatcaaatca atttctcttt tgcatgcttc agttaaatcc accttgattt
     1021 caagataatt ctccacggca agttcctttt ctaaatatat tgcaatatct atgtcactat
     1081 ctgttcttat aaccctttgg gcatatgaac cgaaaatata agcaaatact atattttcat
     1141 atcctatcag tatattttta cacttttcaa tattatccat gcaattctca actcctaaaa
     1201 caaactgcca gtattaagta cgaaatcaaa taattcatat atcaattgta tcataaatta
     1261 tcacaaaagc aagctttaaa cgttcatcct acgcggtttt aaagcattta aaacctgctt
     1321 ctgttacctt gatgtagctt acgatttgca aattcttcat tatttctttc aaatcgtaaa
     1381 ctatatagat agagattgaa ctgtctgctc caaagataaa cattttcttt tacgtattct
     1441 cccaccttat agtgcaatta cttcaggact agtattattc ctcatactga atatccggtc
     1501 agcattatca aagataccat taccgataat aactaatatt tttgcacttt tttttttagc
     1561 tttttgtaat attcaccaaa tattatcaat agctttacgt gctatttcta tccattctgt
     1621 aaagcgtgca gaatctttcc ggcaggtatg tgtatcacgc aaaataagct caattatgcc
     1681 accttttgct ttttgaagtc cagtgaccag ttcttgttct acagcatcag tatcaaaata
     1741 atcaaatgca agaaacgaag gttgaggttt ccatgtcata atataatctt tcccaagtct
     1801 tggtacaaaa tttgaaatat ctgcccaagg acaaactgcg actcgcctga ggtttttaat
     1861 ctttaaaact ccatccagtt ttttaccagg atcgtcgcaa catccatatc cgtttagccc
     1921 aaaattttct aataattctc tctcatattg cagtacaaat tcctcatgca tggctggtga
     1981 cacattggaa aactcttgtg cttcagcagc tgcccagagg tcgcgcaatc taacacaatc
     2041 gggtgtaaaa ccttcatgcg gaagctcatc tgtatatcca ataccacctg tatagtgaaa
     2101 tgtgaaatca ttatttaacg acaataattt ctgattttca tactgctgga gcatatgttt
     2161 tataccatct gtaaagaacc gcatgacatc atgcaccatc ttgggctcat catacaagtc
     2221 catatacata ttatcaagac ctctgtagtc acagtaccaa tgcattatat gaaaactcag
     2281 tcttgttatc cctacaagct taaccggtaa aatatcaccc aagcaactat aaaccaactc
     2341 ataattctca gcgcttgctg ctttatcatg tgtaatagac ggaaatgata gtttttgcta
     2401 atctgaaggt tcattaataa ttggctcatg atgccatgtt ccccctgctt gctttgaatt
     2461 aatacgcttt ggctcaacac cccacataga attatgtata actttttgta catttatatc
     2521 gtcatggcac ggtacatcat caggtatata cttgtgacgt attagacgct gttttaaatc
     2581 atattcaatt tccctcagca gaggggtttc acataccagg gattcatatg gaagcaactc
     2641 attccatgct ccatcaggat gtacaaatac aactggtctt tcagctctag gcgtattgtg
     2701 agaagtccat aactttttcc tttcttcctg ctcaggcaac gcagcaattt cagcaacctg
     2761 cttcgcaagt tcacgcagaa tacttttgtc atgtattaaa ttcttatcag gcatataatc
     2821 actcattgta tatatgttca cctacttcgt atcccaccac tgataaatca aatagcaata
     2881 aacaaatata tttccctgta agaggaaagt ttgagcaaca acagtttaag cctcttattg
     2941 accaattgga ttatctatcc aagcggaaac cctttaatat ccttagaact caggctattt
     3001 tcttaaatat acttgataag ctagcatcat taaccaagtc atcagcatca gaaacccggg
     3061 aacttgcaat tcagattatg gactttattg cagaaaattt taacaaacca ttaacttgga
     3121 accatctttc aaaaaaattt aattactccc ctgattatct tgcccgaata atgaaaaaac
     3181 attatggaat aactctgggg cagtatattc aagagtttag gataaataag gcaaaggagt
     3241 tattagccaa taccgattac cccctttctg ccatatctcg tgaagtaggt tacagtgatg
     3301 tttctttgct ttataaagct ttcagaaatc aggaaggaat tgcacccggt atatggcgca
     3361 caaactgccg gggtgtttta aagcactgaa atctgcatat ctagtcatat ttacatgaaa
     3421 gtaatgcctc ctgttttttt acatagcaac tatctttatt gaccagttgc cttccttcac
     3481 tttctgtttt ggcttaagaa taattctata aagataactg ccccattgat caattaacct
     3541 catatcattt agttgtatgg tttcataaga ataatcaaaa acatcagtgt caaattgcag
     3601 gaaaggtatt cttccttctg acatgttttt cgaaaattct ttttcataaa tgttttctgg
     3661 ttcttctttt gctgaatcca tttcaatacc tttttttaaa tcattttcaa atttttcctt
     3721 agttttaagg catattttac cttttgtttc attaccggaa ttctcttgac ttttataaac
     3781 aagtttgcat ggggtcatat aagttagctt aatatcatcg gacaattcaa acaacttaaa
     3841 atcctctata atttcaatat atttttcgct gtcacgactt aaatggcagc tccttttcca
     3901 ataacagata ccgctttctt cagggtacgc tttatctata ttcattgcaa gcactgcttt
     3961 tctattatca ttatgatata ttacatttgt tgccttataa actctgcctt ccttttgctg
     4021 ataaccgtta actgaaggaa gattatggaa tgacgattgc attgtccaaa ggctgtatct
     4081 ttcctggctg aatgttttcc ctgagtatat ctccacaccg ggatcaacaa tgacaggttc
     4141 ggcattataa taaacgataa aatttcctac atcattatga ttatgacttt cagcattatg
     4201 cccgcctttt gccgcaatat agaaaccttc gtacgaacca tgctgttccc ttgcagccat
     4261 cacctgaata tctttaagcc atacatcacg gatataatag tctactttcc ggccttctgg
     4321 tattttacaa cagctgaatt cagcactgca tttggttgca gtcatatcgg atttagatga
     4381 atcggattta gataaaagta cggtcatcca tttgttatta aaaagcatag gaagttctcg
     4441 tattaaaata ttaggctttc gtgttgccaa ttttccaccc tcatcttctc ttaaaaatgc
     4501 ttctgcttct ttcataaaca ttttcgctcc aatatctgca agtgtatcat cactaatcct
     4561 tcgcccatat ctatacacaa gatcgttaga tagcctgagt ttgcttgatg cgtcagcaaa
     4621 atttatgaaa ttatctttgc ctatatacgc acgatagaaa tatcttccca tatcttttat
     4681 aagaggttca ttatatatgt caattattcc gtcagttgca aaatataact gctcaagtat
     4741 atcaaaaaga gcagccggtg ccattgacca atagcttatt ccctcatcgc aacctccatc
     4801 cgcaggatag cttccaatat atatgtcagt aatttccata gctttcagaa caccctggat
     4861 acgccttttc caatcatctt caagcatcag aaatgctata aggcagcatg aattacatgt
     4921 aggattccag ttattaattg gatgaagtac cttcattctc ggattcatca ttctttgaaa
     4981 tcccatccac catatatcgt cattttcaag gaatggtgtt aatatcctct tatttatttc
     5041 atatttaatt cttctgttca tattaacact cactttatca aatgcagaac ccatcaggta
     5101 atatgcccat gtaagcaaag atgccgttgc agctgagcta aggtcaataa caggatctgt
     5161 tgcatccggt aaaggatccg gccagtttct gtgcatataa ctatttgcag gtatggccca
     5221 ggaagattcc tcacaaatac tccatattcc atttatgata ttatcaatat attcgttttt
     5281 accattgagg cattctgcta aaataagagt ccgtaatacc tgccttttcg cagatacctc
     5341 taatctttcg ggagtatctt catttttttt acatgattct atgaagtcag atgcactaag
     5401 agacggccac ttaaaatttc tatacacttc ggcttcgttt atcaccggtt tagataaaac
     5461 aggcattact aatttccatc tggctctatc atcaattgtg ggaaaaggct tgataccatc
     5521 aacaggtagt agaatattgc tgattttatt accatatcgt tcgataagca ttttatttaa
     5581 cttcctccat ttcaattagt gacataaaat tgattccggt gcaaaaactc attcagcggt
     5641 catcacgttg taatttgtaa cacttcatct tgcattttta ttatttacct aaatttccga
     5701 ccttgctctc atggtataat ttggggtgca ttttatgtac ccttactctt attatctagt
     5761 tgacaaaatt acttcaagtt ggtttcaatg attgactaag gggaataaca aatttatcac
     5821 caggctctgc atcgtaaaga aaaccttttc cttcatcatc ttcatctgcc aatcctctta
     5881 tcaaagtaac atctccgata tcaagagcaa acaggttatt accaattttc tctgcactaa
     5941 taattctata tacagcattt cttatgtctg catattgtac agtttcttct cttaattcag
     6001 gtatgctttt cataacatag ccggttctcc ttgctgcata attttctatg taaatatatc
     6061 tacccttaag tttatggata atcataccta aattattatg atctatgaaa tcttctttgc
     6121 ctgaatcttc tgcatttccg ctatcatctt tatccgaatc tccaaagtct tcattaatac
     6181 ggactataag cctgttgcta aaagaagggc atcgtgtaaa atcttccagc gtacctgtta
     6241 atgctccata tttactgtta aaaattacag atccgtcaac agacatatat gtaccatggc
     6301 aaataaaagc atatgctgtt tctcctccct gttcagaata aacgccatac acgcccgtaa
     6361 aaataattct attgtctacc agatagctgg tttcagggtt caaggtgcta ataatataat
     6421 cagttctacc attatttaaa actaacttaa ttgctgctgt atcaattgtt tctttatttt
     6481 tatcattctt ctctgcagta attttaccta aaatatgaat gctaactttt tctaatgatt
     6541 ttataaacct tttctcttta tatggctcaa ttacactgac aaattgactt tccagatcat
     6601 tgtttttgct tctatgtgct atcatatacc tcaatttttc aggatttcct accttattct
     6661 gcggaggctg cccatccgac agtgcaacac tgttcactct gtttagcatt gtaagcctta
     6721 aatgaatatc tgtatctttc tccaatgctc cccatgtatc ttttatatcc caatctatac
     6781 taaagccagg ttccggatta tatgatcgtt ctacattata taggtaatga aatccactgc
     6841 ctttatatgt tgcagtacag tttttaaggt catcatatct ttccatatac tcaacttccg
     6901 gtccggcata tgtacctcca tcctgttttt taaattcaat gccttcactg cttacattag
     6961 cttctgctcc atggaaacta tagtaatgat cattccctcc ctttacacgg aaaatatcaa
     7021 caatgtatga gttctcacaa tcaaccctga tcatcattat agatcttcta taaagttcga
     7081 tttcagaata agcaaatggt gcttctacat caatgatttt tacatcaaga gtatcttcaa
     7141 aatgttttga aataccggta tacatacgat cttgcttttt tgcatcaaca accactgtat
     7201 tatgactaat agtgttatta ccccattctg ttctgtgact gcaatcatgt cttgcaaatt
     7261 caggatagcc aagatcagga gttaaatcaa gcccgaaggc atatatacca aggttcagtg
     7321 catcctggtg cccatgccat atagtccttc cataatatat ccaagcagct ttacgagtat
     7381 cctcttctat acctggtgct atttcagtat tatcgctgcc atcctttaac accgcataac
     7441 cataccctgt taaatctata ctttctacta gaagctttcc tttattctca ataacagatt
     7501 ttacatcgtt ctcaatttca ccggccaaag gatgaaatat attcaaacaa agaccatcag
     7561 ctttgttttg atttaataga tatgcctgtt gtgctaattc cggatcttta tatcttctga
     7621 atccatcaag tgtaatgctt ttgtttccat ataaaaacgg tttccctgaa cttccgctgt
     7681 cagctatgga agggatatac ttgtcaaggg ttataagtgg atgcaatgct aaatacattt
     7741 ttctgaattt tggactttta tataagttta ttacatttct agccttattt tgttttgtaa
     7801 attcttcatc ctgttcttta taatattttt catagtattc ttcataaagt tcaagaatat
     7861 ctgccagatc ttttaattga ctcaaccaaa gataattata tccaggagca gcttcatttc
     7921 catgcccatc tctatctaca tcatagtata aaacattcaa aatatttccg cctgttacct
     7981 tccaatcagg ctcacccacc aatcctccag gtttaagaag ccacttcatc cattcttctg
     8041 ccgcattctt atcctccaat accagtgccg ccattgccag tgtactctga tgcataccta
     8101 cattaccttt tattctggca tcttttatcc ttggatatac aagcctgatt atattatttt
     8161 caatattttc cctaatatgg tcaatatcgg tttttttatt atcaaacccg tataattcag
     8221 ctttttcatt tagaaaagag ataacatctg tacaatcttt gttgtaaatt tcaggaaaga
     8281 aagcgtcata tgcgctaatt aatttctttg ctacttcaca ttccgatatt gccccggtaa
     8341 tattgccttt ttttgttata ccatggttat gtctgtaccc atcctcaatt ctatactgtg
     8401 aagcatccat atcagggtaa acatccgcca ctctgtccag aattgcaata cctgctcttc
     8461 catatcttgc ttctcctgta tacaaatatg catcctttag agcttcaatt atgcataaaa
     8521 ttgaattttt cctttcacca gatgctccac cgccatccat ccatatatac caatgattat
     8581 agtatgctgc aaaggtatat cttctgcttt tttcatctat tacaccataa ccatcatcta
     8641 cacaccaatt ttcaggttta tcagggtata atgtgttttt aagaaggctt atgtctgcta
     8701 tttcaggata aaataatcca tatttgccta ttccgctttt gtaatatgct tcgaagtcat
     8761 ttgatggaaa aaatgtccca cagttgggac attccaattt ccacggcata tttacactat
     8821 ctgccaccca gggataatgt ccgaatttgt ttattccctt tctgcatacc gggcagccat
     8881 catcgcacac tgctgcactt ctgggtatac tctgggaggt aaccagactc cataaataat
     8941 caattcctaa agacaggtaa tactcagcat tttcaaccga ctcattcatt atatcttttg
     9001 tcgagtagac tcattctttt ctcctttctt gtgagttagg cgaaaattaa gc
//
LOCUS       DAOSDD010000187         8994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2873_length_8994_cov_5.035686, whole genome shotgun sequence.
ACCESSION   DAOSDD010000187 DAOSDD010000000
VERSION     DAOSDD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_8994_cov_5.035686"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /gene="larB"
                     /locus_tag="PK733_14440"
     CDS             <1..491
                     /gene="larB"
                     /locus_tag="PK733_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOJ11774.1"
                     /translation="YLKKDYPVHYNKAGQIAVIGEMPKPDGIGKILVATGGTSDMPVA
                     EEAAFTAEALGNTVIRLYDVGVAGLHRLLSYLEEIMSARVIIAIAGMEGALASVIGGL
                     ADCPVIGVPTSVGYGASMGGISALLSMLNSCASGVSVVNIDNGFGAGYLASMINHISA
                     KP"
     gene            642..1898
                     /gene="larC"
                     /locus_tag="PK733_14445"
     CDS             642..1898
                     /gene="larC"
                     /locus_tag="PK733_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOJ11775.1"
                     /translation="MRTLYFECNMGAAGDMIMAALLELHPDAYGFIERLNKLGIPDVE
                     VVLTKVIKLGITGSQVLVKVRGTEEESHDVHYHGHSNGHSHGYEHSYEHDNNDEHEHS
                     HEHEHHHHAGIEDIDKIIRGLAVSAKVKNDALAVYGLIAEAESRAHGKPVEDVHFHEV
                     GTMDAIVDIVGVCMLIEELNPQIILASPIHIGGGHVRCAHGILPVPAPATAYILRDVP
                     VYGGAIKGELCTPTGAAILKHFVSKFGVLPVMRISNIGYGMGKKDFEAANCVRAYLGE
                     TDKHEKEVVELRCNIDDMTPEGIGFALELLMEKGALDVYTTPIGMKKSRPGVLFTCIC
                     FRDQVDEMKSLILRHTTTLGIREIACKRYTLHREKSIIETKYGPITIKTATGGGFSKS
                     KPEYEDIAKLARENGVSLNEIMDEIR"
     gene            complement(2076..2837)
                     /locus_tag="PK733_14450"
     CDS             complement(2076..2837)
                     /locus_tag="PK733_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11776.1"
                     /translation="MIKVKHLDFSYKKDRIILEQISFDASHGQCIAILGNNGAGKSTL
                     VKCLNHILTPQNGIIYINGEDIRNLKRNEIAKSVAYVAQRNFADKLTVFDTVLLGRKP
                     YIKFEPKQKDIEITESVIKRMGLENFSLRYVDELSGGEMQKVMLARALAQQPGILLLD
                     EPTSNLDLRNQYEVMSVIREIAKQENISVIIVIHDINLSIRYCERFLFIKDNKVYAYG
                     GKEIMTPENISHVYGIKVEVKTVCGMDIVIPLPQD"
     gene            complement(2834..3886)
                     /locus_tag="PK733_14455"
     CDS             complement(2834..3886)
                     /locus_tag="PK733_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ11777.1"
                     /translation="MKTHRTYKEYIHYKYLFILFILLVTILIALLAISAGSAGLPLKL
                     VISTLLGWGTKQSSLIIFNHRLPRIITAVVAGIGLGSVGCVMQSLLNNPLASASTLGI
                     AQGAAFGASFAIIILGAGMQNQTLDGITILNPYLISICAFISSMLSTLVVLGLSRFNK
                     ITPESMVLSGVALSSMFSGATTLLQYFAADVKVAAVVFWTFGDLGRTSWREILIMSVV
                     VILVLTYFIFNRMNFNAIQSGEETAKGLGVNVEFMRLIGMFLCSLAAATIVSFVGIIN
                     FIGLIAPHLMRRCIGNDYRYLLPASAIMGALLLLLSDTVARLAVAPIILPIGAITSFL
                     GAPLFLYLLFKGVGKR"
     gene            complement(3893..5041)
                     /locus_tag="PK733_14460"
     CDS             complement(3893..5041)
                     /locus_tag="PK733_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOJ11778.1"
                     /translation="MKENIIKKITMKKKLIISVLIFLLLLAGCKTNTITNTPGNLPLN
                     SDNASSDFRTIVDAFGREVKVPKKVETTAAIGGAARILTYAGCADKLVGITDMDKKNL
                     PAMPYTVVNAQHFSTIASVGSGGSNDTCYIEELINLAPDVIFGLTDEDTIKNVAEKTG
                     IPTVAVYPTNMFDESFYFALELVGQIMGTNAHSTKVIDYVKSCQMDLDKRTKDIGNDS
                     KPSVYSGAVSFRGAHGIEGTYADYPPFTAIHAKNVVDETGKSGSLLIDLEKLTVWDPD
                     IIFLNPTNMHLVNEDYAKNKAFYNNLKAVKNGNVYSQISYNYNWTNMEIAIANAYYAG
                     KIIYPEKFSDIDPIKKADEIFTTMLGQPFYDKLLEAGYKFDKISIGEK"
     gene            complement(5139..5666)
                     /locus_tag="PK733_14465"
     CDS             complement(5139..5666)
                     /locus_tag="PK733_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOJ11779.1"
                     /translation="MNKQLWDKSVAFHGHECPGLAIGFKACEAAAEKMQLEFSDDEEI
                     VCVTENDACGVDAVQAIFSCTLGKGNLIYRGTGKQAFSFFNRKTGQKFRVCLKPSQNK
                     GMDRDQWKEYLLNSQVDEIFTFSEPKFDLPERARLLKTVVCEICEEGTPEHKAHLQDG
                     KIVCADCFKTYDRGW"
     gene            complement(6026..6337)
                     /locus_tag="PK733_14470"
     CDS             complement(6026..6337)
                     /locus_tag="PK733_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11780.1"
                     /translation="MRERSSKKINNELLHRIDGRKIKYVSLRKGTESGETILGKDGVI
                     NVVDGDIVIICNARTVFRHPVENIGLDELMSLAGVNIRYKDEDTGEDLTLIAYYKYHR
                     K"
     gene            complement(6432..7712)
                     /locus_tag="PK733_14475"
     CDS             complement(6432..7712)
                     /locus_tag="PK733_14475"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOJ11781.1"
                     /translation="MRIREMASRAREASIILAASDTELKNKALVKIAEALKNNKDEII
                     RANKSDVERSQNENISAPLLKRLKFDEPKISDVVNGIYSLIKLEDPVGRSLMTTELDD
                     GLELCKESCPIGVIGVIFESRPDALVQISTLCLKSGNSVLLKGGSEARETNRILAEII
                     SRATKEAGIPEGWIQLLETRADVNEMLKMDEFIDLVIPRGSNEFVRYIMDNSRIPVLG
                     HADGICHCYVHEEADIDMAVKIVVDSKAQYVAVCNATETLLVDEKIATAFLPVLKEEM
                     DKRGVVLTGCEKTRAVIPTEKANEEDWKTEYLDYKLSIKIVNDIHEAINHINAYGSGH
                     TDAIITGNKNAACMFMNLVDSGNVFWNCSTRFSDGFRYGFGAEVGISTNKIHARGPVG
                     LEGLVIYKYKLTGNGHIVEDYANNRKSFKHKRIE"
     gene            8040..8327
                     /locus_tag="PK733_14480"
     CDS             8040..8327
                     /locus_tag="PK733_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11782.1"
                     /translation="MARTKSVDSVKKYTVVLPESCINELKVLSEKKIIDSISAGVREA
                     VAEYLVKVKREIYKKELMEAVKDSDFIKRNEEVIESFKYADKQTEEIIHKW"
     gene            8321..8668
                     /locus_tag="PK733_14485"
     CDS             8321..8668
                     /locus_tag="PK733_14485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOJ11783.1"
                     /translation="MVKMWSIYWTNLDPIIGSEQAGTRPSLVISNDTVNEVMPVVTVL
                     PLSSIKKSSRIYPTEVFIPQTQSSLPKDSVAMIHQIKTIAKVRIGSKCGDINDESIRA
                     NIKYVIKQYFELS"
BASE COUNT         2853 a         1682 c         1578 g         2881 t
ORIGIN      
        1 aatatctgaa aaaagattat cccgttcatt ataataaagc cgggcagatt gctgttatag
       61 gagagatgcc aaagccggat ggaattggaa aaatcttagt tgctacgggg ggaaccagcg
      121 atatgccagt agcagaagag gcggcattta ctgcagaagc cctcgggaat acggttataa
      181 ggttatatga tgttggcgtt gcaggacttc atcggctttt atcttattta gaagaaatta
      241 tgagtgcaag agttattatc gctattgcag ggatggaagg agctttagcc agtgtaattg
      301 gaggacttgc agattgtccc gtcattggcg tacccactag tgttggctat ggtgcttcta
      361 tgggtggaat ttccgcatta ttatcaatgc taaattcctg tgctagtgga gtgagtgtgg
      421 taaatattga taatggattt ggagcagggt atttggcaag catgataaat catatttcag
      481 ctaagccgta agccttataa gcataagaat cgctttgata aattaaggtg ctgtatttcc
      541 tcaaaaagtg ccatttaaag ctcatactac atagatttga gcgataaagg tattttcttt
      601 gattagtctt ttgttgaaaa taatatacag ggggatagaa aatgagaaca ctttattttg
      661 aatgcaatat gggagctgca ggggatatga taatggcagc cttacttgag ttgcatccag
      721 atgcttatgg ttttatagaa aggttaaata aactgggcat tcccgatgtc gaagttgttt
      781 taactaaggt cattaaatta ggaattacgg gttctcaggt tttagtaaaa gtgagaggga
      841 cggaagaaga aagtcacgat gttcattatc atgggcatag taatggtcat agtcacgggt
      901 atgagcatag ctacgagcat gataataatg acgaacatga acatagtcac gagcatgagc
      961 atcaccacca tgctggaatt gaggatatcg ataagattat aaggggatta gctgtttctg
     1021 ctaaggttaa aaatgatgcc ctcgctgttt atggattaat tgcagaagca gaaagtcgag
     1081 cacatggaaa gcctgtagag gatgtgcatt tccacgaggt aggtactatg gatgcaatcg
     1141 tagatattgt aggtgtatgt atgctaatag aagaactaaa tccccaaata attcttgcat
     1201 cacctattca tatcgggggc ggtcatgtac gttgtgcaca tggtattcta cctgtaccgg
     1261 ctcccgctac tgcttatatt ttgcgggatg taccggttta cggaggtgca attaagggag
     1321 aactatgtac tcctaccggt gcggccatct taaaacactt tgtttctaaa tttggtgtgt
     1381 tgccggttat gagaatatct aatataggat atggaatggg gaaaaaagat tttgaagcag
     1441 caaattgtgt aagggcatat cttggagaaa cagataaaca cgaaaaggag gttgtagaat
     1501 tgcgttgcaa tattgatgat atgacccccg agggaatagg cttcgcgctt gaacttctca
     1561 tggagaaggg agctttagat gtatatacaa ctccaattgg tatgaaaaaa tcccgacccg
     1621 gcgtgctctt tacatgcata tgcttcaggg atcaagtaga tgaaatgaag tctttaattt
     1681 tgcgccatac aacaacactt ggaataagag agattgcatg caaaagatat actcttcaca
     1741 gagaaaaatc aatcattgaa actaaatatg gacctattac cataaaaaca gcaacaggag
     1801 gtggtttttc aaaatctaag ccggaatatg aggatatagc aaagcttgca cgggaaaatg
     1861 gagtatctct gaatgaaata atggatgaaa taagatagag aataacttgc caatgaaaga
     1921 acaaattatt ttatacaaat ttaattgtca attaagcaat aagttaaggg gagcatttac
     1981 tggatttgaa gaaagaatta ccaatggtgc atttgttaag ttattaaaaa tgcaccatta
     2041 atacaaaaat aaagcaagac aaataagaga taacatcaat cctgtggtag cggtattact
     2101 atatccattc cgcaaactgt tttgacctct acctttatac cgtatacgtg gcttatattt
     2161 tcaggagtca ttatttcttt acctccataa gcatagactt tattatcttt aataaaaagg
     2221 aaacgttcac agtaccttat agatagattt atgtcatgaa ttacgataat tacactaata
     2281 ttttcctgtt tagcaatttc tcgtattacc gacataacct catactgatt acgcaaatcc
     2341 aaattactgg taggttcatc taaaagcaag ataccgggct gctgtgccag tgctcgtgcg
     2401 agcataacct tctgcatttc gccaccggat agttcgtcta cataacgcag ggaaaaattc
     2461 tctaatccca tacgtttaat tacagattct gtgatttcta tatctttttg tttaggctca
     2521 aactttatat agggtttacg acctaaaaga actgtatcaa acacagtaag cttatctgca
     2581 aaattccttt gtgctacata tgcaacagac tttgctattt catttctttt aagatttcga
     2641 atatcctcac cgtttatata aattatgcca ttttgtgggg ttaaaatatg gttaaggcat
     2701 ttaacaagcg tactctttcc ggccccatta tttcctagaa tggcaataca ttgaccatga
     2761 cttgcatcaa atgaaatttg ttcaagaata atgcggtctt ttttataaga aaaatcgagg
     2821 tgtttgactt taatcatctt ttaccaaccc ccttaaatag aaggtataag aataaaggtg
     2881 cacctaaaaa ggaggttata gctccaatag gcaaaataat tggagctact gccaaacgtg
     2941 ccacagtatc acttagtaaa agcaataatg ctcccattat ggctgatgca gggagtaaat
     3001 accgataatc attacctata caccggcgca ttaaatgtgg tgcaataagc ccgataaaat
     3061 taataattcc aacaaaagat acaatagtag cagcagcaag agagcaaaga aacataccaa
     3121 tcagacgcat gaactccaca ttaactccta agcctttagc agtttcttcc ccgctttgaa
     3181 tggcattgaa attcatgcga ttaaatataa aataagttag cactaaaata actaccacag
     3241 acattatcag aatttctctc caactagtgc gccctaaatc tccaaatgtc caaaaaacta
     3301 ctgcagcaac ctttacatcg gctgcaaaat actgtagtaa agtcgttgca cctgagaaca
     3361 tggaacttaa tgcgacaccg gaaagaacca tggattcagg tgtaatcttg ttaaagcgag
     3421 ataatcccag cacaaccaaa gttgatagca ttgagctgat aaaagcacaa atagatatta
     3481 aatagggatt taaaatagta atgccatcaa gagtttggtt ttgcataccg gcacctagaa
     3541 taataattgc aaaagatgca ccaaaggcag caccctgtgc aattcccaat gttgaagcag
     3601 aggcaagtgg attgttaaga aggctttgca taacacatcc tacagatccc agaccgatgc
     3661 cggcgacgac ggcggtaatg attctaggca agcggtggtt aaaaataatc aatgatgatt
     3721 gtttggtgcc ccaccctaaa agtgttgaaa taacaagttt tagaggaagc cccgccgagc
     3781 ctgcactaat agccaataat gcaattaata tagtcactaa taaaataaat aaaataaaca
     3841 agtatttata gtgtatatat tctttataag ttctgtgagt tttcatcaat gtctattttt
     3901 ctcctatgga aattttatca aacttatacc cagcctccag gagtttgtca tagaaaggct
     3961 gacctagcat tgtagtaaaa atctcatctg ccttttttat tggatcaata tcagaaaatt
     4021 tttccgggta tattattttt cctgcataat aggcattagc aatagcgatt tccatattag
     4081 tccaattata attataagaa atttgagaat agacattccc gttttttact gcttttaaat
     4141 tattataaaa agctttattt tttgcataat cctcattaac taaatgcatg ttagtaggat
     4201 ttaagaaaat tatatccgga tcccatactg taagtttttc caagtctatc aacaaagaac
     4261 cggatttacc tgtttcatct actacgtttt tcgcatgaat agctgtaaat ggaggatagt
     4321 ctgcatacgt accttcaata ccatgtgcgc cacggaaact tacagctccc gaataaacag
     4381 agggtttact atcattacca atatcctttg ttctcttgtc taaatccatt tgacagcttt
     4441 taacgtaatc aattactttg gtagaatgtg cattagttcc cattatttgt ccaactaatt
     4501 ccaaggcaaa atagaaactt tcatcaaaca tattggtagg ataaaccgct accgtaggta
     4561 tgcctgtttt ctctgctaca tttttgatag tatcttcatc agttagtcca aaaataacat
     4621 caggtgcaag gtttattagt tcttcaatat aacatgtatc attactgcct ccactgccaa
     4681 cagaggctat agtggaaaaa tgctgagcat ttactactgt gtatggcatt gcaggtagat
     4741 tctttttatc catatctgta atacctacca gcttatctgc acagcctgca tatgttaata
     4801 ttcgagctgc accaccaata gcagcggtag tttctacttt tttaggaacc ttaacctctc
     4861 gaccaaaagc atcaacaatg gtacggaagt cggatgaagc attatcagaa ttcagaggta
     4921 aattaccagg tgtattagta atagtattag ttttacaacc tgctaagagt agtagaaaaa
     4981 ttaaaacact aattattagt ttctttttca ttgttatctt tttaattata ttttctttca
     5041 tagttacaat attttcctcc ttgtaaaatg gttttataaa taatatttca atggggataa
     5101 tgatgttttg ttccacgttc ctagttataa ttattgcttt accatcctct atcataggtt
     5161 ttaaagcagt cggcgcagac tattttaccg tcttgtaaat gagctttatg ttctggtgta
     5221 ccttcttcac aaatttcaca aacaacggtt tttaatagcc ttgctctttc aggcaaatca
     5281 aatttaggct ctgaaaaggt gaaaatctca tctacctgag aatttaaaag atattccttc
     5341 cattgatctc tatccatgcc tttattttgc gaaggcttta agcataccct aaatttttgt
     5401 ccggttttac gattaaaaaa tgaaaatgct tgtttaccgg taccgcggta gataagattg
     5461 cctttcccca aagtacaact gaaaatcgcc tggactgcat ctaccccaca ggcatcgttt
     5521 tctgtaacac atactatttc ttcatcatcg gaaaattcaa gttgcatttt ttctgctgca
     5581 gcttcgcatg ccttaaatcc gatagcaagc cccggacact catgcccgtg aaaggctaca
     5641 gatttatccc ataattgttt attcattcca acaccccgtt catataatag ctccttctat
     5701 taaaactcta tataaaaatc tgtatataga gcttcgataa aactttgtgc aaaaacccca
     5761 taaaatagaa aaaagctcaa atagagcttt ctagctttca tttaatagct tcatgctatt
     5821 ctattcaggc cgatatagaa ttttataaag aaacaaatat taaaactttt aactcttgac
     5881 agctatataa taaaactttt ctcacaaaga tttcgtatct ttgaaatgta tttcgtatac
     5941 atatattctc actgtttaaa agtatagtaa ttttccgggt atttgtcaat agagatagag
     6001 ggatatgagg gcgagatagc tttttttatt ttctgtgata tttataataa gctatcagtg
     6061 ttaaatcttc tcccgtatct tcatctttat atctgatatt tactcctgca aggctcataa
     6121 gctcatctaa ccctatgttt tctacaggat gtctgaaaac cgtcctggca ttgcagatta
     6181 tgacaatatc gccgtcaaca acatttataa ctccatcttt gccaaggatt gtttcaccac
     6241 tttctgttcc tttacgtaaa gatacatact ttatttttct cccatcgatt ctgtgcagga
     6301 gttcattatt aatcttttta ctgcttcttt ccctcattat acctgacacc gcccggtttt
     6361 gtgttttaac cagctacgtt tctgctaaat aggttatgtt tatgctgagt aagttatttt
     6421 acagctttag gttattctat ccttttatgc ttaaaagatt tcctgttatt tgcataatct
     6481 tcaacaatat gcccgttacc tgtaagttta tatttataaa ttacaagccc ctcaagtcct
     6541 acaggacctc ttgcatgtat cttgtttgtg cttattccaa cctccgctcc aaagccatac
     6601 ctgaatccgt cactaaaccg ggttgagcaa ttccagaaaa cattacctga atcaacaaga
     6661 ttcataaaca tgcaggcagc atttttattt cctgtaataa tggcatctgt atgtcctgaa
     6721 ccataggcat taatgtgatt tattgcttcg tgaatatcat tgacaatttt aattgataat
     6781 ttgtaatcca gatattcagt tttccagtct tcttcattag ccttctcagt tggtattacc
     6841 gctctggttt tttcacatcc tgtcagaact actcccctct tatccatctc ttctttcagt
     6901 actggcaaaa aagcagtagc aattttctca tccaccagaa gagtttctgt tgcattgcat
     6961 actgctacat attgggcctt agaatcaact actatcttta cagccatatc tatatcggct
     7021 tcctcatgta catagcagtg gcatattcca tctgcatgac ccaatacagg aattcttgaa
     7081 ttatccatta tatagcgtac aaattcattg gatcctctag gtataaccaa gtcaatgaat
     7141 tcgtccattt tcagcatttc atttacatca gcccttgttt caagaagctg tatccagcct
     7201 tccggtatac ctgcctcctt tgttgcccgg gaaataattt ctgctagaat gcgattagtc
     7261 tcccttgctt ctgaccctcc cttaagcagt acactattgc cgcttttcag acataaagtt
     7321 gatatctgca ctaaggcatc aggcctggat tcgaaaataa ctcctatcac acctattggg
     7381 caactctcct tgcaaagttc caatccatca tccagttccg tagtcatcaa actcctgcca
     7441 acaggatctt ccaacttgat taagctatat attccgttta ccacatctga tattttgggc
     7501 tcatcaaatt taagccgttt caataaggga gcggaaatat tttcattttg gcttctttcc
     7561 acatctgatt tgtttgctct gattatctca tctttattat tttttaaagc ttcagctatt
     7621 ttaaccaaag ctttgttttt tagttctgta tcggaagcag ccagtataat tgaagcttcc
     7681 ctggccctag atgccatttc tctaattctc atacctatca tgcccgcctc tcttttgcat
     7741 tatatatact actttattgt cttgtttatt gccttgccaa aggaaagtgt ataaattata
     7801 ccggaagcta tctcatttaa tagttacctt actcataact aaagtaacga gagttcagca
     7861 agtattttcc aaaattggca aagcattata aacattatat aattaaactt gtttaataca
     7921 ttttaatata tttatagaag taattcaacc tggatgtttt gaagaatact gttttattgt
     7981 tgcaattcat ggttgaatat tatacaatac ttatatacca taatttatgg aggtactata
     8041 tggcacgaac aaaatcagtt gatagtgtaa agaagtatac tgttgtttta ccggaatcct
     8101 gtattaacga attaaaagtg ttgtctgaaa aaaaaattat agactcaata agcgcagggg
     8161 taagagaagc tgtggctgaa tatttagtaa aagtaaaaag agagatttat aagaaagaac
     8221 taatggaggc cgtaaaggac tctgacttta ttaaacgaaa tgaagaagtt attgaaagct
     8281 ttaaatacgc tgacaaacaa acagaggaga taatacacaa atggtaaaaa tgtggtctat
     8341 atactggaca aacctagatc ctattatcgg ttcggaacag gctggcacaa gaccatcact
     8401 ggtcatttca aatgatacgg ttaacgaagt catgccagtt gttacagtgc ttcctctctc
     8461 ttcaataaaa aaaagcagcc gtatatatcc tactgaggtt tttattccac aaacgcaatc
     8521 ctcgctccca aaggattcag tagcaatgat acatcagatc aagacaatag ctaaagtaag
     8581 aattggctca aaatgtggtg atattaatga tgaaagcatc agagccaata ttaagtatgt
     8641 aattaagcag tattttgaat tatcataaaa agatagaatt gctttgcaag tacagccaca
     8701 taggagtttt gagtgtttac tgcatatgaa agctaagaat tcatatccac caaagatatc
     8761 aaattttgat gagaaaaaac caaagcctaa acctgtttga tattaaaggt taacagagag
     8821 gtcttgtata tctcatatca acatctcctt cgaagcatta tattcctcct atcctacagt
     8881 gatttgtact aacgttacaa ccctctaggt tgtacagtaa agtgacttaa ttttccctta
     8941 actattttaa attgtgaacc ctttccgctt gacttataat catagttttg ctga
//
LOCUS       DAOSDD010000188         8974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2882_length_8974_cov_5.386815, whole genome shotgun sequence.
ACCESSION   DAOSDD010000188 DAOSDD010000000
VERSION     DAOSDD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_8974_cov_5.386815"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..783)
                     /locus_tag="PK733_14490"
     CDS             complement(511..783)
                     /locus_tag="PK733_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOJ11784.1"
                     /translation="MSKSCYICNGEMEKKAISINTGWGKYKLIVEGVSSYKCPKCGEV
                     VLEGKDAIMLQKLSRSLADLEDQQKPDILNLTEVAKLLTNNNQTTY"
     gene            complement(921..1550)
                     /locus_tag="PK733_14495"
     CDS             complement(921..1550)
                     /locus_tag="PK733_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11785.1"
                     /translation="MNKLSAVRSSPVILDACVLMVGIDKQNKDINYSFENMRDSHLQA
                     LFEYSQNIKIHEFVMGELDQKRQDFIKQYIGKNVEVVSEGDLYGKDPQYTTLFNKIAR
                     HELFNYRRDETHNRGEVYSLAYAAFHNISYFSTRDGSAMIVLDEMPELSDIELVGFEY
                     VLAIGFLISKCRNKDMDKRLKSLYKAYCEPSIRRGVIPHTFAEFLNSLK"
     gene            complement(1550..2494)
                     /locus_tag="PK733_14500"
     CDS             complement(1550..2494)
                     /locus_tag="PK733_14500"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOJ11786.1"
                     /translation="MNDLFYQAFIEKYSQERYDFSINALEPDEVNQVKSLAQEKRHEF
                     GIAPIGTDIFRFIRDKENNIYFEKESFDNNDLDAMLYLPDPNKDNAYIILNSSQPLIN
                     QIFATAHEYYHYIKDLEYVRKNPKVCSLSSLKDKSEQKASRFAAEFLLPESSLRKSIE
                     RWLLLIKKRNFGDAEFNEVAALCYCLTVQYCMPLKAVMFRIYEEGYIDDISVYLSNYN
                     FIKQTLKESKTQFAKRIQELMATENPYIYEVMYRLIPKAYNTGYVSLDKVESDVSQLG
                     LVMDNMMEFLDIKDCEDEEEIDDSLRESLLIKLSEMGQ"
     gene            complement(2494..2835)
                     /locus_tag="PK733_14505"
     CDS             complement(2494..2835)
                     /locus_tag="PK733_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11787.1"
                     /translation="MIINFNSELFVQKLKEFKGNKSQNDVANELGINNRSTISLLENG
                     KQIPSLEVLQKLCEKVNLPVDTFFMKETQSPVLMMMGQLKETDRSKLENVINRIKIRE
                     KYIAISKRCDN"
     gene            complement(3138..3716)
                     /locus_tag="PK733_14510"
     CDS             complement(3138..3716)
                     /locus_tag="PK733_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11788.1"
                     /translation="MPRRKLICPKCGSTNALNIIYGMPAPEAIEASRRDEVILGGCSV
                     LPDSTLHICRDCNYEWEKYCEISILPSIIYIKAYIGGFWGPNCRIEADAINGKLKSSY
                     THCDQEEFADDEIDIAPEAWKGLLRALKNCDFEYWVDKYEDPHICDGTQWSVEVQLDT
                     GKKIMKYGSNMFPGRWKQFCRAMTRFSGVKYK"
     gene            complement(3758..4231)
                     /locus_tag="PK733_14515"
     CDS             complement(3758..4231)
                     /locus_tag="PK733_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOJ11789.1"
                     /translation="MKPVKLKDIVDEMEMQMDEYHKYLNKETGEIVTVSTEELTIAEE
                     SEEDDDFSEYPDWQRDSIKDALDVIVNWENYIELPDKWEINEYDIMEEFCGSIRNDRI
                     SNVLYTAIQGRGAFRRFKDAIIRFEVEDDWYKFREEALKQIAIEWCEENEIKYIV"
     gene            complement(4253..5230)
                     /locus_tag="PK733_14520"
     CDS             complement(4253..5230)
                     /locus_tag="PK733_14520"
                     /inference="COORDINATES: protein motif:HMM:NF039671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6155 family protein"
                     /protein_id="HOJ11790.1"
                     /translation="MKKDEHLLSITELRNILKQWSRESLIDLLVDCYKINSQVKEYIS
                     AKYAGQDTIEHIFETYKNKVHDVFFPRSMSAQFKIGEARKAVNDFKKICSDEKLVIDL
                     MLYYVEMGVEFTNTYGDIGESFYNSVESMYQSVIAAINKHKNPATFNMLSKRLKAVVD
                     DTDGIGWGFHDNLSDMYHDIKWLDAGDIDVDLAALQTTKTYISSRLEKRKNLPGIDGS
                     KLNIEEITSRVIDADEIFLDKMDKQGRSYSNDEEYDFIVKKTGFATELIELILWQRYC
                     YEMENDFWQYTEGECKKCGNNKLYLKEVPGEDYADRVVCKECGTELSRD"
     gene            complement(5309..>8974)
                     /locus_tag="PK733_14525"
     CDS             complement(5309..>8974)
                     /locus_tag="PK733_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11791.1"
                     /translation="LRVLSKSISESRDRKKMCELTDYLCLKEYDDILLELDFGLCMKS
                     EDQNEVECALERIITWATNTDIDDEDKLLIAEFFIKHRNDTKSAYKWVENIEQPELCK
                     SEKIGWDNKTLSSFSHRIRLNRILAALGIYVDPVKIIPNTTDERQFGIVLFERMLIRL
                     STIWGKAWAGETLTPGEVIREVRPAIIIFNRSYDETRDWNPWYQIRQVAIDYFRFLIR
                     SVAAHGIECVVAIADEFNKQWQDKETKKYWPTEWQRHISLNIYKHDHSSDRLINQLCL
                     IESQMDVLDDIYSRVNGMMEQAFAWIEAGECDRAKSLIPMIINASFGIYNDKDSQFNR
                     WVDWLEIVNDIHKENSEERIKRFAGALAVLEQAGRGCDVQDGAAKLIEIAAKRHCGYA
                     FYLRSWLMDHHAIHYVSSLEGIILAALKETEPPIKVIFSLVNHLLIPVQNSASEEIAE
                     LLALKCCQCLSEKEALSTIQTLIQAIETKAFPTERFIWWDGILKGLKATGVNAECVES
                     KINEKSVLAQIESKPKIALLSGKKLTEADAEKRVINYSSLLTLLDSIKDSEHMSLEKI
                     LLRVIDNLTYEQVNIIYNRLNNFDRNSGAIMYLAKRMASTGHKTEAIKIIQEEYKRSP
                     SSGWDVHYDGGSRLQIIETLISIDADYGRVIALKSLINDYISDVRYPENFIRNLNRLL
                     NVLFDIVPVVEVWSELERHIYQLYDFSNAEDYPEIAMESIEELSFSEMLIKIMTNDYL
                     LSVAEIREEAHGAICDAAFSNTADHILKARLLKLMNDDVRSQSSALAVLEAVMAKRSD
                     FVSGFNNEIIQLCRHRNCIVRNLALSIADLLDVYIEEECGEKELPPVYKIELPELQMT
                     KWTYPLDVSAEDDFGKHINDPIESVRPFLDYYEVIAEYTDIPLQNLIARASRIINSRE
                     MTNKYIMFSESELRSWLQAAELKLAFYRPGAIGALYTLGYIIGELIDAAFLDTSDFMG
                     LIIHDPVLSVTKPVPRPVEVLLPEIVDKEHFFQKEDWMKKGREDLSLILNCMDNGNII
                     LGELSRFINLNWEKPTEYRLSMVCHPDWPELLDIEDAYNFFPYSSGWYAREYPYLDNV
                     ANKFSAVIYSQPLQVEIGKTEWLAFNPCLAFHLGWKLSTEGLFRWVNKNGEIMVESIY
                     WKDGPINRLPPRMAEICSEGWIVAASKKAAELISNRIGNAIYLKALVRQHSNRDERDV
                     SRNSITQREKWKHTKNV"
BASE COUNT         2596 a         1952 c         1206 g         3220 t
ORIGIN      
        1 gcactttttt atttcagtgc cttgtaatgt ggagccctca atcggaatcg aactgtgacc
       61 tcatctttac catggatgcg ctcaaatttc gatgtgatcc gtagaaggat tgatattact
      121 gcatttgaag gcttgtaata cccatgattt tataaaaagt aaaccatttg taaaccattt
      181 cactttttcg gatgctcaga agctgtgtcc agcagtaaga gtttacggca attcacaaga
      241 aacttcttaa tcaattcact gtttttatcc ataacaaagt tataaattca aagggaacca
      301 gacgcatcca attccccgag gtgtagtccg cagacatcca ccgcaattcc aagaaaatcg
      361 taacattatt tattttgctc cctaccgggc tataaaaaat catacttcct accggacgtt
      421 ttcataatac tacattatac ataccttttc aactataatt tagatacaac cattttgcct
      481 ttcaacggat aattgtgcat tatttgtctg ttaatatgta gtctgattat tatttgtaag
      541 taactttgcg acttctgtca agttcaaaat atctggtttc tgctgatctt ctagatcagc
      601 cagacttctg ctcagttttt gtagcataat agcatctttg ccttcaagaa caacttctcc
      661 acatttgggg catttatatg aactaacacc ttctacaatt aatttatatt tgccccatcc
      721 agtgtttata gatatagctt tcttttccat ttccccatta cagatatagc aactcttgct
      781 catgagaaat acactacctt ctttaagaaa tgttattcat cttcacaata ccaggcataa
      841 ccctattgga attatcttac ccctatccta tgtggttatc tttttaatct tccttttgtc
      901 tcagccttaa ttactgagtt ttattttaat gaattaagaa actcagcaaa agtatgaggt
      961 ataactccac gccttatact tggctcgcag tacgctttat acaatgactt tagtctttta
     1021 tccatatctt tatttctaca tttacttatg agaaacccta ttgctaacac atattcaaat
     1081 ccaaccaatt caatatcact aagctcaggc atttcatcaa gtacaatcat tgcactacca
     1141 tctcgtgtag aaaagtaaga aatattatga aatgcagcat atgcaagact gtatacttca
     1201 ccacggttgt gagtttcgtc tcttctatag ttaaacaatt catgtctggc aattttatta
     1261 aacaatgttg tgtactgtgg atctttaccg tataagtctc cctcactgac aacctctaca
     1321 tttttcccaa tatactgttt aataaaatct tgccgttttt gatcaagctc acccataaca
     1381 aattcatgaa ttttgatatt ctgtgaatat tcaaataatg cctgcaagtg ggaatccctc
     1441 atattttcaa agctgtaatt aatgtcctta ttctgtttat caatcccaac catcaataca
     1501 caggcatcca gtattaccgg acttgaccgc actgcactaa gcttattcat tattgcccca
     1561 tctcacttaa ctttattagt aagctttctc ttaagctatc atctatttcc tcttcatctt
     1621 cacagtcttt aatgtcaaga aactccatca tattgtccat aacaaggcct agctggctta
     1681 catcactttc aactttatct aatgagacat agccagtatt atatgctttg ggaatcaacc
     1741 tgtacatgac ctcataaata tatggattct ctgtagccat taattcctgt attctttttg
     1801 caaactgcgt cttactttcc ttaagggttt gttttataaa attataattt gatagatata
     1861 ctgaaatatc atcaatatat ccttcttcat atatacggaa catcacagct tttaaaggca
     1921 tacagtattg taccgtcaaa cagtaacaca atgctgctac ttcattaaat tctgcatctc
     1981 caaaatttct tttttttatt aataaaagcc atctctcaat tgactttctt aatgaagatt
     2041 ctggaagtaa gaattctgca gcaaatctac ttgccttttg ctcactttta tccttcaaac
     2101 tcgacaacga gcatactttt gggtttttac gaacatattc cagatctttg atataatggt
     2161 agtattcatg tgcagtggca aaaatctggt taattaatgg ctgacttgag tttaaaataa
     2221 tgtatgcatt atctttattg ggatctggta gatataacat agcatccaga tcattgttat
     2281 caaacgactc cttctcaaag taaatattat tttctttatc tcgaataaat ctaaatatat
     2341 cagtccctat aggtgctatt ccaaactcat gtcttttttc ttgtgcaaga cttttaacct
     2401 gatttacttc atcaggttcc aaagcattga tgctgaaatc atacctttct tgagaatact
     2461 tctctataaa ggcttgataa aataaatcat tcattaattg tcacaccttt tgctaatagc
     2521 aatatacttt tccctaatct taatcctgtt gattacattt tcaagttttg acctatctgt
     2581 ttcttttaac tgtcccatca tcattagaac agggctttgc gtttccttca taaagaaagt
     2641 atcaactggc aaatttactt tctcacataa tttttgcagt acctcaagag atggtatctg
     2701 cttaccattc tccaatagtg aaatagttga cctattgttt ataccaagtt cgttggctac
     2761 atcgttttgc gatttattgc ctttaaattc ttttagtttc tgtacaaaca gttcgctgtt
     2821 aaaatttata atcactagaa accctccccc atgtgttaat tattataaca ccatgcaatt
     2881 tgtttatcaa tacatgttat gattattaac actattattt gactattata cgaaaattat
     2941 ttattaattt tattaaattt atcggcattc ctttcatacg agcatctaat agttttaata
     3001 gtattggttt tctattttaa accctggaag aaataatgag tatcaaagtt agaattttag
     3061 aaaaatatta aaacaatcag ctaattgttc atttttgccc attattatta acgccataga
     3121 gcagagacta tttcatatca tttatatttt actcctgaaa atctagtcat tgccctgcag
     3181 aattgtttcc acctaccagg gaacatattg ctcccatatt tcattatctt ttttccagta
     3241 tcaagttgta cttcaacact ccattgagtt ccatcacaaa tatggggatc ctcatattta
     3301 tcaacccagt attcaaaatc acagtttttt aaagctctca gcaatccctt ccaggcttca
     3361 ggagcaatgt caatctcatc atctgcgaat tcttcctgat cacagtgagt atagctactc
     3421 ttaagctttc catttattgc atcagcctca atacggcagt taggccccca aaaaccacct
     3481 atataagctt tgatatatat aatagaaggc aaaatactga tttcgcaata tttttcccat
     3541 tcataattac agtccctgca tatatgcaat gtactatctg gcagcacact acagccacca
     3601 agaataactt cgtcccttcg ggatgcctct atcgcttcag gtgcaggcat gccgtatata
     3661 atatttagtg cattggttga accgcatttt gggcagatta actttcttct tggcatattt
     3721 ttgtttcctc agcatcatat tttacttttc ttctctgtca aacaatgtat ttaatctcgt
     3781 tttcctcaca ccattcaatg gcaatctgct ttaatgcttc ttcacgaaac ttgtaccagt
     3841 catcttcaac ttcaaaccgg attattgcgt ctttaaacct tctaaaggct cctctacctt
     3901 gaatggcagt ataaagaaca ttgcttatcc tgtcgttcct aatagatcca cagaactcct
     3961 ccatgatgtc gtattcgttt atttcccact tatccggcaa ttcaatgtag ttttcccagt
     4021 ttactattac atccagtgca tccttgatgg aatcccgttg ccaatctgga tactctgaaa
     4081 agtcgtcatc ttcttcagat tcttctgcaa tggtaagttc ctctgtgcta acggtgacta
     4141 tttcaccggt ctccttattt aagtatttgt ggtactcatc catctgcatt tccatttcgt
     4201 ctacaatgtc tttcagtttt acaggtttca tcttcattcc tcacattccg gcctaatctc
     4261 ttgaaagctc cgttccacat tctttgcaaa caaccctgtc tgcataatct tccccaggaa
     4321 cttcctttag ataaagctta ttgtttccgc actttttaca ctcaccttca gtatattgcc
     4381 aaaaatcgtt ctccatttca tagcagtatc tctgccaaag aataagttct ataagttctg
     4441 tagcaaatcc tgttttttta acaataaaat catactcttc gtcattgctg taacttctac
     4501 cctgcttgtc cattttgtct aaaaagattt catcagcatc aatgactcta cttgtaattt
     4561 cctcaatatt cagcttactg ccgtcaatgc ccggcaaatt ctttcttttc tcaagccttg
     4621 aggatatata tgtcttagtt gtttgcaaag ctgcaaggtc aacatcaata tctcctgcat
     4681 caagccactt aatatcatga tacatatcac ttaagttgtc atggaatccc cagccgattc
     4741 cgtcggtatc atcaactaca gctttaagcc gcttgctaag catattaaag gttgccggat
     4801 tcttatgctt attaattgca gctataacag attgatacat actttccaca ctgttataga
     4861 agctctcgcc gatatctcca tatgtgtttg taaactcaac acccatttct acataataca
     4921 gcatcaggtc aatcacaagt ttttcatccg aacatatctt cttaaaatcg ttgactgctt
     4981 ttctggcttc tcctattttg aactgagcac tcatgctcct tggaaagaat acatcatgca
     5041 ctttattttt ataggtttca aatatatgtt ctattgtatc ctgaccggca tattttgcag
     5101 aaatgtattc tttcacttgt gagtttattt tgtaacaatc tacaagcagg tcaatcagtg
     5161 actcccggct ccactgttta aggatattcc ttagctcagt tatacttaga aggtgttcat
     5221 cctttttcat ttttcggttc tccatttctt aatctactta ccagcatagt atttctactt
     5281 taaagtgttt agtttttatt actacttatc atacattttt agtatgtttc catttttctc
     5341 tttgcgtgat actgtttcta ctaacatctc tttcatcacg attgctgtgc tgacgaacca
     5401 aggcttttaa gtatatggca tttcctattc tgttggaaat caattcagct gcttttttag
     5461 atgcagctac tatccaacct tcagaacata tttccgccat tcttggcggt aatcgattaa
     5521 taggcccatc cttccaatat atactttcga ccattatttc tccattttta ttaacccagc
     5581 gaaataaccc ttctgtactg agcttccatc ctaaatgaaa tgcaaggcat ggattaaacg
     5641 ctaaccattc tgtctttcca atttcaactt gcagtggttg tgagtatatt actgcagaaa
     5701 acttatttgc cacattatcc aaatatgggt attcccttgc ataccaacca ctgctataag
     5761 ggaaaaagtt ataggcatcc tcgatatcaa gtaattccgg ccaatctgga tgacaaacca
     5821 ttgacaaacg atattctgtt ggtttttccc agtttaagtt tatgaaacga cttagttctc
     5881 caagaataat attaccatta tccatacaat ttaaaattaa agacaggtct tccctccctt
     5941 ttttcatcca atcttccttt tgaaaaaaat gttccttatc aacaatttct ggaagcagca
     6001 cttctacagg ccttggtact ggtttggtaa ctgaaagaac cggatcatgt atgattaaac
     6061 ccataaaatc agaagtatcc aaaaaggcag catcaataag ttcaccaata atatacccta
     6121 gtgtatataa tgcaccaata gctccaggcc tataaaaagc taattttaat tctgctgctt
     6181 gtaaccatga ccttagttca ctttctgaga acatgatata cttatttgtc atttcccttg
     6241 agtttattat tcttgaggct cttgcaatta aattctgcaa tggtatatca gtatattctg
     6301 ctattacttc ataataatct agaaatggac gtacactctc aatcggatca tttatgtgct
     6361 ttccaaaatc atcttcagct gaaacatcta aaggatatgt ccacttagtc atttgaagtt
     6421 ctggcaactc aattttatat actggtggta gctctttttc gccacattcc tcttctatgt
     6481 aaacatccaa taaatccgct atactaagag ctaagtttct tacaatacaa tttcgatgtc
     6541 tgcacaattg gattatttca ttattaaagc cgctaacaaa atcactgcgt ttggccataa
     6601 ctgcctctaa aactgcaaga gcactacttt ggcttctaac atcatcattc atcaatttta
     6661 ataaccgtgc tttcagaata tgatctgcag tgttcgaaaa ggcagcatca catatagctc
     6721 cgtgtgcctc ttcccttatt tctgcaacag ataataaata atcatttgtc ataattttta
     6781 ttaacatctc tgaaaagctt aattcctcta tgctctccat agcaatttca gggtagtctt
     6841 cagcgtttga aaagtcataa agctgatata tatgcctttc taattcactc catacttcta
     6901 cgactggtac aatatcgaaa aggacattta atagcctgtt gagattacga ataaaattct
     6961 caggatatct cacatctgaa atatagtcat ttattaaaga tttcaatgct ataaccctac
     7021 cataatcggc atctatggat ataagggttt caataatttg gagtctacta cctccatcat
     7081 agtgaacatc ccatcctgag ctaggagaac gcttgtattc ttcttgaatt atctttattg
     7141 cttccgtttt gtgacctgta cttgccattc tcttcgcaag atacattatg gcacctgaat
     7201 ttcgatcaaa attgtttaac ctattatata ttatattcac ttgttcatat gtcaagttat
     7261 caataactct taataaaatc ttctccaaag acatatgctc agaatcctta atagaatcta
     7321 aaagcgtcag taacgagcta taattaatga ctcttttttc tgcatctgct tcagttaact
     7381 ttttcccact taacaaagca attttaggct tactttctat ttgtgctaaa acactttttt
     7441 cgttaatttt actctcaaca cattctgcat taacgcctgt agcttttaat ccttttaata
     7501 tcccatccca ccaaataaat ctttcggttg gaaaggcctt tgtttctatt gcctgtatta
     7561 gagtttgtat agtacttaat gcctctttct ctgataaaca ctgacagcat tttaaagcaa
     7621 gcaactcagc tatttcctcg gacgcactat tctgtactgg aatcaacaaa tgatttacta
     7681 agctgaatat aacttttatt ggaggctcag tttcttttaa agcagcaagt ataattcctt
     7741 ctagtgaact tacataatga atagcgtggt gatccattaa ccatgagcga agataaaatg
     7801 catacccaca atgccttttt gctgctatct ctataagttt agcggctcca tcttgaacat
     7861 cacacccccg tcccgcctgc tcaagcacag ctaatgcacc agcaaatctt ttaatcctct
     7921 cctctgaatt ttccttatgg atatcattga ctatttctaa ccaatctacc cacctattaa
     7981 actggctatc cttatcatta tatatcccaa atgaggcatt gattatcata ggaattaagc
     8041 tttttgcccg gtcgcattct ccagcctcaa tccaagcaaa agcttgctcc atcatcccgt
     8101 ttactctact gtaaatatca tccaacacat ccatttgtga ttcaattaag cagagttggt
     8161 taatcagcct atccgatgaa tgatcatgtt tatatatatt taatgatata tgtctttgcc
     8221 attctgttgg ccaatacttc tttgtttctt tatcttgcca ttgcttatta aattcatcag
     8281 caatagcaac aacacattca attccatgtg cagctactga tcggattagg aatctgaaat
     8341 aatcaatagc cacctgcctt atttgatacc acgggttcca gtctcttgtt tcatcataac
     8401 ttctattaaa tattataata gcaggccgta cttctctgat aacttctcct ggtgtaagcg
     8461 tttctccggc ccaggccttt ccccatatcg ttgataatct aataagcatt ctttcaaaaa
     8521 gtacaattcc aaattgcctt tcgtcagtgg tatttggtat tattttcaca ggatctacat
     8581 atatgcctaa agccgccaat atacgattca atcgaattct atgtgaaaaa gatgacagtg
     8641 ttttattatc ccaccctatt ttttcgcttt tacatagttc tggttgctca atattttcta
     8701 cccatttgta tgcagacttt gtatcatttc tatgcttaat gaaaaattcc gcaattaata
     8761 atttgtcctc atcatctata tcagtatttg tagcccatgt aattattcgt tctaaagcac
     8821 attcaacttc attctgatct tcgcttttca tacaaaggcc aaaatccaat tccaacaaaa
     8881 tatcatcgta ttcctttaag cataaataat ctgtaagttc acacatcttt tttctatctc
     8941 tgctttcgga aatgcttttg gataataccc gtaa
//
LOCUS       DAOSDD010000189         8952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2894_length_8952_cov_5.295605, whole genome shotgun sequence.
ACCESSION   DAOSDD010000189 DAOSDD010000000
VERSION     DAOSDD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_8952_cov_5.295605"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK733_14530"
     CDS             <1..250
                     /locus_tag="PK733_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11792.1"
                     /translation="GGCVMILVEEGAVDALVAKLESEYYAPRGLEPGLTVCVPVKGSG
                     VLELVLGNATKDNNVEQVPETVRNLSNDGEKLEKKALQ"
     gene            353..559
                     /locus_tag="PK733_14535"
     CDS             353..559
                     /locus_tag="PK733_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11793.1"
                     /translation="METSEKVLFLKIYLSNNAALWDLKFEKPDFFKTLPGTKHNGMGD
                     NRSFERVWKDFVELGEKEEVTSPA"
     gene            684..1844
                     /locus_tag="PK733_14540"
     CDS             684..1844
                     /locus_tag="PK733_14540"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HOJ11794.1"
                     /translation="MKSNGDIVPFLKQKDYIMVNNDLGGGSFGKTVLLQDPFIDELFV
                     AKKYEPVYEEIKEKFYKNFLDEIKILYKLNHRNIVRIYNYYAYEDIFTGYILMEYIDG
                     TNIGKYITEYTGIWDPISLDDLFTQLIDGFCYIEERSIIHRDIREGNILIDSNGTAKI
                     IDFGIGKMYEKIAQDRDSLVAVINRTDLDILPHEYYKGIYTSQTDMFYLAELFNRLLQ
                     GANEQDLLDFSYQDILVKMMEKRPENRYATFANVKEAIGKHDFINMEISQEDKNTYQT
                     FANLVYQTLTSFINEQKFNYDADMFIAKLEKALRHNLFEDSIQKNADVISSIVTSAYR
                     YNNKVDIPCHVVKNFLDWFGSSTTQSQKLILANIISKLSIINVEEEEPELPF"
     gene            2053..2532
                     /locus_tag="PK733_14545"
     CDS             2053..2532
                     /locus_tag="PK733_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ11795.1"
                     /translation="MHKLLSWMAVILWMSLIFYLSHQPAAKSSELSTSITEVIIDTIE
                     TVAPQVKIEKGDLHYIVRKNAHFFYYLVLGILVVNALRRSGIYGYKSILLALLICILY
                     AVSDEAHQIFIPGRSGEIRDVIIDSTGSNVGIFGYLVVSWVAGSRNRKVVRTSSKMK"
     gene            2678..3250
                     /locus_tag="PK733_14550"
     CDS             2678..3250
                     /locus_tag="PK733_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11796.1"
                     /translation="MNMLKKVSENYDLMGTIVDYTQCINGHINDTYHIKVKSESGKCE
                     EYIFQRINGYVFKEPIKIMSNIKEISLHVQPKITKCDCDIIHFLDNKDGKNYTCYEDG
                     FWRICRYVNNSVTYETAEDRKVLRSEGYAFGRFQCLLSDLPMDKLHDTIPNFHNTKKR
                     LNDFFETVALDPIGRAKDVEADIRFFENNR"
     gene            3271..3624
                     /locus_tag="PK733_14555"
     CDS             3271..3624
                     /locus_tag="PK733_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ11797.1"
                     /translation="MQENGELPLRVTHNDTKYNNILMDKDTCEPLCVIDLDTVMPGLS
                     TYDLGDAIRFAANTAVEDETDLSKVELDMNKRKNHNLDRARCQIKLAQDMINKYDEMC
                     NIVQKNVISILSSEI"
     gene            5127..8291
                     /locus_tag="PK733_14560"
     CDS             5127..8291
                     /locus_tag="PK733_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11798.1"
                     /translation="MLRIENFFQDSSYKEIYLKNIDNIENKLKSAVQLNTPLYNFTDH
                     GTNHSDEINTRLTKIFYNLFTNDDTKIKLNNAELYSLISAIYLHDIGMDMLKKDEIIS
                     MFTRKNYKNQFHKYLADSVDIKDIELLKENSVEFFDFIRRKHHIISAMWIMSNRQYEG
                     NALYLPLIDSFVLPIALIAFAHNEYIDILTKPFYNNTQVNGEIIQVKFLSYLLRIGDS
                     LHADRSRCNINVLNYKDIPVESKIHWFRHYYTKSISIENQSIDILFEFPDEEGWSEDV
                     EQYFISEAKYWINQNITDITINRLFEDNNRNNLLKCILSDRKEYLYSVRSVDKEIKQK
                     IIEILGNKVQLVVKQEKDIYINTYEYKFNEQLIHNKYDGSLNRSLKDIFVQPRLYEFS
                     EHKKDDGKNRKKKRMDIETLLNDEFNYIISGKQGIGKTTLIRYMFLTTRNSNYVPFIF
                     DFSEIEYNTDNILCKKIKSIIGNDSFKINIDIELQNGNCLLFIDNVNKDSIYFRHILE
                     FCSQYPQNKIIMTQKENEEEEISSLENVFEDKVIMGNIKELFIHYLGIYELRIIVNKW
                     FAGSEKDSYSIFSSIYNYILENNLPRTPLLYSLFLSINEKDSDFIPINIATLFDKFSD
                     IYLGKYANLFYNYRSTYDYNRKKYILKELAKYMVDNNKKKLHISEYYEFIDELFGTNG
                     FGITIDKDELLADLKYSNLFARENDHVKFTFDCFLFFFYAKRVVETDKSSDVLFNNIK
                     IEKFSSIIIFYTGLLMNCSELLEECSLYLLDMTNINKDAINKKVNDIFNDNRKSLFHN
                     IDIKSMNEVEIEESINRQSEHEELIEYTGRKKQEEKFGEQEHSFLTMLIQVCSMLKNS
                     EYIDNKLKTEVLELCIDCYTFILCELICNFEKFSKQEEYSDKITEDLKYSAITLFTLV
                     IESIVHSTIGTSMLEEQLRELMKKSKNVLCDFIYCMLSAELALNGYLEYILNFAKRTQ
                     NFLLLDCLLLKLQIIFEERNFNKKPAEKKRMLDIIRFIFEKILKEKNKNARYKDNANR
                     FIENISKRKLAYELKYMPFMIDNK"
     gene            8770..>8952
                     /locus_tag="PK733_14565"
     CDS             8770..>8952
                     /locus_tag="PK733_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11799.1"
                     /translation="MSTKMSLQQFFNPDMKVTNFVEYADKIVIKIKSSTKTQFCPTCG
                     RESDYHQVTYKRVIDDL"
BASE COUNT         3261 a         1174 c         1695 g         2822 t
ORIGIN      
        1 cggcggctgc gtaatgatcc tggtggaaga aggcgctgta gatgcgctgg tagcaaagtt
       61 ggaaagcgaa tactatgcac ccagggggct agagcccggg ttgacagtat gcgtacctgt
      121 gaaggggtcc ggggttctgg aactggtact gggtaacgca actaaagata ataacgtgga
      181 acaggtacca gaaactgtgc gaaatttgtc gaatgacggc gaaaaacttg agaagaaagc
      241 gttgcagtaa aaagatgaaa taccttatcg aaggaaccgg tagggtattt tttgtactgt
      301 atgatagtat tctatggaga aagtgttatt aaataaatat tagtgctcag ccatggaaac
      361 ctccgaaaaa gtactctttt tgaaaattta cctcagcaat aatgcagctt tgtgggatct
      421 aaaattcgaa aaacctgact ttttcaaaac cctcccaggc acgaaacaca acggaatggg
      481 ggataatcgg tcttttgaaa gagtttggaa ggattttgtt gagttgggtg agaaggagga
      541 agtaacctct ccggcttagc cgataattta ggatattata gttatagcaa taatgggagt
      601 tttaaataag taaccgataa attatgtatt ctttccttaa ttgctttaat agaaacatct
      661 aaatttgaaa tggaggaaaa ttaatgaaaa gtaatggaga cattgttcct tttttaaagc
      721 aaaaagacta tataatggtt aataatgatt taggtggcgg ctcgtttggg aaaacagtgt
      781 tgttgcaaga tccgtttatt gacgaattat ttgtagcgaa aaaatatgaa cctgtgtatg
      841 aagaaatcaa ggaaaagttt tataaaaatt tccttgatga aatcaaaatt ctttataagc
      901 tcaaccatcg aaatattgtt agaatatata attactacgc ttatgaggat atctttaccg
      961 gatatattct gatggagtat attgatggaa caaatatcgg aaagtatata actgaataca
     1021 ctggaatctg ggatcctatc tcgctagacg atttgtttac gcagttgatt gatggctttt
     1081 gctatattga agaacgatca attattcata gggatattcg tgaaggaaat atattaattg
     1141 acagtaatgg aacagctaaa atcattgatt ttgggattgg aaagatgtat gaaaaaatag
     1201 cccaagacag ggacagccta gttgcagtaa ttaatcgaac ggatttagac atattgcccc
     1261 atgagtacta caaaggaatt tatacatctc aaactgatat gttttacctt gcggaattat
     1321 tcaatcgttt acttcaaggt gccaatgagc aggatttgtt ggacttttct tatcaagata
     1381 ttttggtcaa gatgatggag aagcgaccag aaaatagata tgcgaccttt gccaacgtta
     1441 aggaagcaat aggaaaacac gattttatta atatggaaat ttcccaagaa gacaagaata
     1501 cataccaaac atttgcaaac cttgtatatc agaccctcac gtcttttata aatgaacaaa
     1561 aatttaatta tgatgctgat atgtttatag ctaaactgga aaaagctttg cgtcataatc
     1621 tctttgaaga ttctattcag aaaaatgcag atgttatcag cagtatagtc accagcgcat
     1681 atcgctataa caataaggtt gatattccat gtcacgttgt gaaaaatttt cttgattggt
     1741 ttgggtcttc aacaactcaa tcgcaaaaac taattttggc gaatataatt tccaaacttt
     1801 ctataattaa tgttgaagaa gaagagccag aacttccatt ttaatcaaga gaataataaa
     1861 taaaatttcg tactactgag cttgggaaac caacgaaaaa atcactttcc cctgtaaatt
     1921 gaagcccgaa acccgtataa cttggttagc atttttttaa aatggtactt tttcgggggt
     1981 ttccctggca tcaaatacaa cggatacaac atagaatata agaaatatat agataaaagg
     2041 gaatatgtgc atatgcataa attgctatct tggatggctg taattctttg gatgagcttg
     2101 attttttact tatcacatca accagcagct aagtctagtg agttaagtac cagcataaca
     2161 gaagttataa tcgatacaat agaaacagtt gctccacagg tgaagattga aaaaggagac
     2221 cttcattaca tagtcaggaa gaatgctcat ttcttttatt atttagtact tgggatactg
     2281 gtggtaaatg ctttgagaag aagtggcata tatggctaca agagtatatt attagctctt
     2341 ctgatttgca ttttgtatgc tgtatcagac gaagcacatc aaattttcat acccggcaga
     2401 tcaggagaaa ttagagatgt aattatagat agcactgggt ccaatgttgg tattttcggt
     2461 tatttggttg tgagctgggt tgctggaagt aggaatagga aagtggtaag aacgagcagt
     2521 aaaatgaaat aggtgactcg ttacaatata agatcgggta aataaaatat aggcatttca
     2581 ggtttattct agatttattc cagggttgtt caggtttata atgaacatag agattattta
     2641 attatagggg agataggatt gtatgggggt tcaaactatg aacatgttga aaaaggtgag
     2701 tgaaaactat gatttgatgg ggacaatagt agattacact caatgtatta atggacacat
     2761 aaatgataca taccatatta aagtgaaaag tgaaagcggc aaatgtgagg aatacatctt
     2821 tcaaaggatc aatgggtatg ttttcaaaga accaattaag ataatgagta atatcaagga
     2881 aatttcactt catgtacagc ctaagataac taaatgtgac tgtgatatca ttcattttct
     2941 agataacaaa gatggtaaaa attatacctg ctatgaagat ggtttttggc gaatttgccg
     3001 atatgtcaat aactcagtta catatgaaac agcagaagat cgcaaggttt tacgtagtga
     3061 agggtatgct ttcggccggt tccaatgctt actgtccgat cttcccatgg acaagctcca
     3121 tgacactata ccaaattttc acaatacaaa aaagagacta aatgattttt ttgaaacagt
     3181 ggctttagat cctattggaa gagcaaagga tgttgaagca gatataaggt tctttgagaa
     3241 taaccgttaa ttgcatcaaa actggtagat atgcaggaaa atggagagtt gcctctacgt
     3301 gtaactcata acgatacaaa atataacaat atattgatgg ataaagacac ttgtgagccc
     3361 ctttgtgtta tagaccttga cactgttatg ccggggctta gtacatatga cttaggtgat
     3421 gccatccgct ttgctgccaa cacagcagtg gaagatgaaa cagacctatc aaaagttgag
     3481 ttggatatga acaaaagaaa aaatcataat ttggacaggg caagatgcca gatcaaactg
     3541 gcccaggata tgattaataa atatgatgaa atgtgcaata tagtgcagaa gaatgtgatt
     3601 agcattttat caagtgaaat ttagaaaagt ggatgagctc ttatgcttgg ttccggaaca
     3661 cgaagatatc tcatataaat acatccttga atattttctt ataaaagaag caacaattcg
     3721 acaaaattca gcaacataat acaaatcaca tgtaatataa tttctctata gtattaacat
     3781 aagcagaaaa cttcatttga tagcatttaa ggtaatatgt tacaaaactt attttaacaa
     3841 taagtgggaa atggaatctg ctttttaatt ttgctatacc attaaaggcc tatcaaaatt
     3901 gatcatcgac tcgagcgtgt caaatctttt aggactgcat ataaaagttc aggtagtgta
     3961 aaaagagggg aattttgcac gaaagggcga agccataggt tgagcttaag tttacaggtt
     4021 aagtgtagaa aaaagtcatt tagatcatat attcttgcaa taggctctgc attaatcact
     4081 ttttgcagag aacctcaaga tattatgtag tgtaggatat gctttgggcc gtttcaaata
     4141 cttgctgtcc gaatacaagg agaaaattat agtttagata ggacaagatg ccagatcaaa
     4201 ctggtccaag atatgtactg cagagcctgg cacattttac aacggtgaca ggaaaaagtg
     4261 gctatttaag caggttttgg gttttgtcgt tgtgtttttc agcttggtgc caaatgcaat
     4321 atatacaaca aaggatacaa ggaatatata gataaaaagg tacaagtaca aatgcataaa
     4381 tgattaccct ggatgactat gattctttgg atgagcctga tttttaactt atcacatcaa
     4441 ccagtgtatt tcggacgcat ccagacaaca taactagttt atgtggacat tttgctgtgt
     4501 taatacaata ttacctccag caagtgataa tattgataaa tgaaggcaat atcaactgca
     4561 ttttattttg atgtggtaat gtacctgtca ccatccgaat tttaccgaat gaggaggtta
     4621 ggttagtaac agaatgatac acgaagtatt gtaaagttaa ataacattat ctatggatag
     4681 attatagatc ataaacaaag gtgaaatgaa ggctctggat taaatgagaa aggctgtaaa
     4741 gaattaaaaa gtagaaccta ggacaaactt ttttttgtct cctaggaagg acaaagtttg
     4801 ttaaacagaa tgttccagat caaggcttta gtgttgctct tttattttag tagtaacttg
     4861 ctgaatttca attctacgag tcttgctact ctcgtagcct ttacatttaa tatcatatcc
     4921 ggagcaatac ttacatttat ttttaaaagt ataatttata cataacttgt cattgttttc
     4981 atccataaaa atatcacatc cattaaatag ttttttgtag ataattattg ccaattttaa
     5041 gggaatatac ctatgcatta attttttatc agtaaaaatg aagtgagaga atattagata
     5101 gtatcatagt ggagtgaggg atataaatgt tgagaataga aaattttttt caggatagta
     5161 gctataaaga gatttatctt aaaaatattg ataatattga aaacaaactc aaatctgcag
     5221 ttcaattgaa tacaccattg tacaatttta cagatcatgg aacaaatcat tctgatgaaa
     5281 ttaatacaag gttgacgaag atattttata atttatttac caatgacgat acaaagatta
     5341 agctaaacaa tgcagaatta tactcattga tttccgctat atatttgcat gatataggca
     5401 tggatatgtt aaaaaaagat gaaattatta gtatgtttac aagaaagaat tataaaaatc
     5461 aatttcataa atacttggcg gattcagttg atataaaaga tattgaactt cttaaagaga
     5521 attctgtgga attttttgat tttataagaa ggaagcatca tataatatct gctatgtgga
     5581 ttatgagtaa tagacagtat gagggaaatg ccttatactt accattaata gattcttttg
     5641 ttctacctat tgctcttata gcctttgctc acaatgaata tatagatatt ttaactaaac
     5701 cattttacaa taatacgcaa gtcaatggcg aaattattca agtgaaattc ctatcgtatt
     5761 tgctaagaat tggcgattca ttgcatgcag ataggagtag atgcaatatt aatgtattaa
     5821 attataaaga tatcccggtg gaatccaaga ttcattggtt tagacactat tataccaaga
     5881 gcatatctat tgaaaatcaa agtattgata ttttatttga gtttcctgat gaagagggat
     5941 ggagtgaaga tgtagaacaa tactttatta gtgaagctaa atattggatt aatcaaaata
     6001 taacagatat cactatcaat cgtttatttg aagacaataa tagaaataac cttttaaaat
     6061 gtatcctttc agaccgtaaa gagtatttat attctgtcag gtctgttgat aaggaaatta
     6121 aacaaaaaat aattgagata ctaggaaata aagtacaact tgttgtaaaa caggaaaaag
     6181 atatatatat aaatacctat gaatataagt ttaatgaaca attaattcat aacaaatatg
     6241 atggaagttt aaatagaagc ttaaaagata tctttgttca accgcgtcta tatgaatttt
     6301 ccgagcataa aaaagatgat ggtaaaaata gaaaaaagaa gagaatggat attgaaacat
     6361 tactaaatga cgaatttaat tatattatta gcggtaagca gggtataggt aaaactactt
     6421 taattagata tatgtttcta actactagga atagtaatta tgtaccattt atttttgatt
     6481 tttcagaaat tgaatataat actgataata tattatgtaa aaaaataaaa agcattatag
     6541 ggaacgattc atttaaaata aatatagaca tagagcttca aaatggaaat tgcctgttat
     6601 tcattgataa tgttaataaa gattctatat atttcagaca tatcttggag ttttgcagtc
     6661 aataccctca aaataaaata attatgactc aaaaagaaaa tgaggaagaa gagattagtt
     6721 ctcttgaaaa tgtatttgaa gataaggtaa taatggggaa tataaaggaa ttgtttatac
     6781 attatcttgg tatctatgag ttaagaatta tagtaaataa atggtttgca gggagcgaaa
     6841 aagattccta ctctatattt agctcaattt ataactatat cttggagaat aatctaccaa
     6901 ggacaccact attatattca ttatttttga gtattaatga aaaagacagt gattttatac
     6961 ctattaatat tgctacatta tttgataagt tttcggacat ttaccttggg aaatatgcca
     7021 atcttttcta caattataga agtacatatg attataatcg caaaaaatat attctgaagg
     7081 aattggcaaa atatatggta gataacaata aaaagaagtt acatatcagt gaatactatg
     7141 agtttattga cgagcttttc gggacaaatg gttttgggat tacaatagat aaagatgagc
     7201 tattggcaga tttgaagtac tcaaatctat ttgcaagaga aaacgatcat gttaaattta
     7261 catttgattg tttcttattt ttcttttatg ctaaaagagt agttgaaaca gataaatctt
     7321 ctgatgttct ttttaataat attaagatag agaaattttc aagtattatt atattttata
     7381 ctggtctgct aatgaactgt agtgaattat tagaagagtg tagtctgtat ctgctggata
     7441 tgaccaacat aaataaggat gctattaaca aaaaagttaa tgatattttt aatgataaca
     7501 ggaaatcctt gtttcataat attgacataa aatctatgaa tgaggttgaa atagaagaat
     7561 ctataaatag acagagtgag catgaagaac ttattgaata tactggcagg aaaaaacaag
     7621 aggagaaatt tggtgagcag gaacatagtt ttcttacaat gcttattcaa gtttgtagca
     7681 tgttaaaaaa ctctgaatat attgataata agttaaaaac agaagtactg gagttatgta
     7741 tagattgcta tacctttata ctatgtgaat taatttgcaa ttttgagaag ttttcaaagc
     7801 aggaagaata ttctgataaa ataacagagg atctcaaata cagtgctata actttgttca
     7861 ctttggtaat cgaaagtatt gtgcatagta ctataggtac ttccatgctg gaagagcagc
     7921 ttagagaatt aatgaaaaaa tccaaaaatg tattatgtga ttttatttat tgcatgttaa
     7981 gcgccgaatt ggctctgaat ggataccttg aatatatctt aaattttgct aaaaggactc
     8041 agaattttct gttgttggac tgcttgcttt taaaactgca aataattttt gaagaaagaa
     8101 attttaataa aaagccagct gaaaagaaaa gaatgcttga cataatacgt tttatatttg
     8161 agaagatttt aaaggagaaa aataaaaatg ccaggtataa agataatgct aatcggttta
     8221 ttgaaaatat aagtaagaga aaattagcat atgagcttaa atatatgcct tttatgatag
     8281 ataacaaata ggttcggagc ctgtcacatt ttgcaacgac gcataaaaag tgagcgctta
     8341 agtaggattg gggtggtagt gttttctgcc aggcacgaaa cacaacagat acaacatagg
     8401 atataagaaa tatatagata aaaaggaata tgtgcatatg cataaattgc tatcctggat
     8461 agctgtaatt acttggatga gtatgatttt ttacttatca catcaaccag tagctaagtc
     8521 tagtgagtta agtactggca taacagaagt tattatcaat acaatagtaa cagttgctcc
     8581 ataggcgaag attgaaaaag cagatcttca tcatactgta aggaagaacg ctcatttttt
     8641 gtattattta gcacttggga tactgtgcgt atttcggatg catccgggtt cttccgcaat
     8701 taaggttgac cttaccttcg ggaactagta aaatatatct attggaacaa taattctttg
     8761 ggggtattta tgtcaactaa aatgtcactg caacaatttt ttaatccgga tatgaaagta
     8821 acaaattttg tagaatatgc ggataaaata gttataaaga ttaaatctag taccaaaaca
     8881 cagttttgtc caacgtgcgg gagggaatca gactatcatc aggtaacata taaacgtgtt
     8941 attgatgatc ta
//
LOCUS       DAOSDD010000190         8944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2896_length_8944_cov_4.974463, whole genome shotgun sequence.
ACCESSION   DAOSDD010000190 DAOSDD010000000
VERSION     DAOSDD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_8944_cov_4.974463"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..283)
                     /locus_tag="PK733_14570"
     CDS             complement(80..283)
                     /locus_tag="PK733_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11800.1"
                     /translation="MRSREDLLFDLKLVDNLVRSSFPNSKLDFYLAGEAACILAGYLD
                     RATRVFSYIRLFIQQGRYYCFKN"
     gene            complement(294..746)
                     /locus_tag="PK733_14575"
     CDS             complement(294..746)
                     /locus_tag="PK733_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11801.1"
                     /translation="MFPEKTISQIKFEIKQIDKLLRAYEELFNKCQDSEPELIEITAL
                     ASVLHSFYNGIEKIFEIIAKRIDKAIPQGKQWHRDLLIQMAKDLPSRKPVISKNMMTE
                     LLKYLAFRHFYRHSYSFFLSWEELEKLVNSIFDVWESLKTEISFFMDG"
     gene            complement(736..1041)
                     /locus_tag="PK733_14580"
     CDS             complement(736..1041)
                     /locus_tag="PK733_14580"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ11802.1"
                     /translation="MLENVIEKVPERYRNDLKRAVKILKSEGCKDVFLFGSLVSGGVD
                     ENSDIDLAIRGCPIGRYFSILAKLMVELDHPVDLVNLDKKDDFSRHLLEEGELIYVS"
     gene            complement(1302..1913)
                     /locus_tag="PK733_14585"
     CDS             complement(1302..1913)
                     /locus_tag="PK733_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ11803.1"
                     /translation="MGNNGYSEKEIAIFNGLIELIRNGANPYSIKVSDIAEASEVGKG
                     TIYDYFKSKEEAISRAILYSINKEAEYAYTRIKSTEKFRDKFYEILHIMAESMENNKS
                     TFNMLIATGSVQEFYEYLVDYECDFSELMEIFNCAIEHLLQTGISEGLIDNNETPYYQ
                     AMVVRGTVAAFSGYISHMDWYATTCLEEAMDSAYRMLIKALGC"
     gene            complement(1917..5639)
                     /locus_tag="PK733_14590"
     CDS             complement(1917..5639)
                     /locus_tag="PK733_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ11804.1"
                     /translation="MLSKFSVKRPYTIAVAVIVVLILGVISFINMQTDLLPNIDLPYV
                     VITTSYPGASPEEVELVVTRPIEQVVARTNNIKNVSSISRENSSVVILEFNNDVNMDS
                     AIIEINSNLDLIKGAWSDSVRLPMIIRINPDMLPIMVASADVEGMNVAEISKMVSEKV
                     IPELESVNGVASVRGVGLLEENIEVTISEGKIDNLNKKILDSIDSKMAEAEDKLLKAK
                     REVDEGKATLESRNKSESEKIAEGEKELTKAFEQISAAEKGLETGKLELIKNKNDLES
                     ALQEINKNEQELIAVEKALLALGKNISEEDKVKLAGIQKGLMDISGKKAEMAAAINEL
                     SNKIEGLEKDKALLVKQREGLNEKKEQIDEGKVLLAGEMSKARTQLIDTEKTINENLK
                     ELEISKEAAFKRASLDGVISTNMISGILAAQNFSMPAGYVKEEGTDYLVKVGNKIKDM
                     DEIKKLLLFDTGVEGVGKVYLSDVADIQMKDNSKDIYAKVNGNDAVMLTFQKQSGFST
                     AEVAKSIRERANKIESENDGFSFTALMDQGVYIDIVVASVLKNIMYGGLLAILILLVF
                     LRDTRPTVIIALSIPISIVFAVAMMYFTGVNINIISLSGLALGVGMLVDNSIVVIENI
                     YRLRSEGESASAASIEGAKQVSGAIISSTLTTVCVFLPIVFVKGISKQLFTDMGLTIA
                     YSLFASLIVALTLVPAMASSMLKSTENRTRNIYDTFLDGYEKMLRWSLKYKAVIMIAV
                     VALLVLSAYYAASMGTSFIPDMEAPQMSITLKMPKESTYEDTIAMSDTVIDRIMGIEG
                     IKTIGAFQNQGMGMGIFGSSSGRAGGNTMSMYLILDENKGVSNAEIEKEIKRLTDGLN
                     AEIAIDSSNMDMSALGGSGIEVIVKGRELDTLRDIALDISKILGETEGTMEVASGTDE
                     NSTEIRITVDKEKSMEKGLTVAQVFSSVNSGISRGKSATTISGISKDYPVIVIDGENQ
                     SIMRKDLEDLKIKSNKNGVETEVRLGDIAAITEEKGLSSIRRKSQERYISVTAAIDAQ
                     HNIGLVSRDLENKLKDYKLPDGYRIEFAGENIMINDSLRDLTYMLLLAAALIYLIMVA
                     QFQSLLSPFIVMFTIPLAFTGGLLALAITGNNISLIAMLGFLVLSGVVVNNGIVFVDY
                     TNQLRDKGLSTKEALVSTGRTRMRPILMTAITTILGLSTLSAGVGVGSEMLQPLAIVS
                     IGGLIYATVLTLFVVPIMYDSLHKNKMPKVLNEGDV"
     gene            complement(5751..6872)
                     /locus_tag="PK733_14595"
     CDS             complement(5751..6872)
                     /locus_tag="PK733_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOJ11805.1"
                     /translation="MFKLPQDYTVDVRILEQLLEYEYLTTSYKIFWFSGMFKEIVRGN
                     RQISFRRVVCRMIATAWYPLLQYHLNFGVSDKLNEIVAMIYKKYGISSDVKEEELLLF
                     LDNMEDKEIEQKIIEFYRYVPYRLISPFYSEVLAGLKDAEKNKVILDLSHSSPKALYQ
                     IIMPDKAILVNENWFEYIYRNQNIVAGWLNYKMIYYLQKKNPNVPAIPFKLSPPYQRD
                     LNAAQKYWSEVMKHIGINDIYTNKPLTSDNFEQYGGISIDHFIPWSFVLHDELWNLVP
                     TFRNINSSKSNKLPKVDLYIDRFCDIQYSAFNIIRKNKAFRNYLEDYLTINKKLDLNS
                     LLYNNISKHEFSDSIKSNIIPLFQIAYNQGYDLWCNDVI"
     gene            7025..7756
                     /locus_tag="PK733_14600"
     CDS             7025..7756
                     /locus_tag="PK733_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOJ11806.1"
                     /translation="MEVPSTEVQNNFGQYMKFAFYEDIIVTRNGKRAVVIKRYEEDNE
                     KGIKGTILAEKAQAYNLEDLKISYEDFIKLTEESDNRYEYIDGEVYLLASPSYNHQSI
                     IVEILNTMYNRFKGEKCKPLTAPFDVTLLKEDNKNVVQPDILVICDTVNINEKGRYTG
                     IPSLVLEVLSETTKHKDLLKKLDLYIHSGISEYWIVNPFKKEIYVYYIEDKAISEYEV
                     YKGNEGAKSILFKGLEISLEQIFEF"
     gene            7983..8138
                     /locus_tag="PK733_14605"
     CDS             7983..8138
                     /locus_tag="PK733_14605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HOJ11807.1"
                     /translation="MAGKQENNYQYAIFAGGCFWCMVPPFQSMDGVVEVIAGYIGIAG
                     YAKLKFT"
     gene            complement(8211..8384)
                     /locus_tag="PK733_14610"
                     /pseudo
     CDS             complement(8211..8384)
                     /locus_tag="PK733_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8493..>8944)
                     /locus_tag="PK733_14615"
     CDS             complement(8493..>8944)
                     /locus_tag="PK733_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11808.1"
                     /translation="RKRRDINGPEIGREPKSKGNLYVFKVKNFHAKTIWIQVDILGES
                     TLDDLHEVIYSNFNLDWGHLYSFFMSGKFWDNASEYHDPQADGKRKADKIRIDSLNLK
                     EGSKFAYLYDYGDENKFEIECKRIEQPENGVKYPKISKRSKKFIEEE"
BASE COUNT         2779 a         1783 c         1274 g         3108 t
ORIGIN      
        1 gacacgccgt aggtgtacct gttttcaggg aaccagaagc tcgtcatttt aatgaagatg
       61 tagttcacga gataatctat caatttttga agcaataata tcttccctgc tgaataaata
      121 aacggatata tgagaaaacc cttgtggctc tatcaagata gcccgccaat atacatgctg
      181 cctccccggc aagataaaaa tcaagcttcg aatttgggaa actgcttcga actaaattgt
      241 caaccaattt taaatcaaaa agcaaatcct ctctgcttct caatacaaac acctcatcca
      301 tccataaaaa aagatatctc tgtttttaat gattcccaaa catcaaatat tgagttaaca
      361 agcttttcaa gctcttccca actcaagaaa aacgaatatg aatgcctgta aaaatgtctg
      421 aatgctaaat attttaataa ttcagtcatc atgtttttac ttattactgg ttttcttgat
      481 ggtaaatcct tagccatttg tattaataaa tctctatgcc actgtttacc ctgtggaatt
      541 gccttatcta tcctttttgc aataatttca aatatctttt ctattccatt atagaatgag
      601 tgtaatacgg aagcgagcgc agttatttct attaattctg gttcgctatc ttgacacttg
      661 ttaaaaagct cctcatatgc tcgaagtaat ttatcaattt gtttgatttc aaatttaatc
      721 tgggagatag tcttttcagg aaacataaat taactcccct tcttctaaaa ggtgcctgga
      781 aaagtcatct tttttatcca ggttaactaa atctacggga tgatctagtt ctaccattag
      841 ctttgctaaa atactaaaat atcttccgat tgggcaacca cgaattgcca aatcaatatc
      901 tgagttttca tcaacacctc cgcttactaa agaaccaaat agaaaaacgt ccttacatcc
      961 ttcagattta agtattttta ctgcacgttt taaatcattt ctatatcttt caggcacttt
     1021 ttctataaca ttctctagca taacttttcc tccttcttta tgataactta tatatatttt
     1081 accacatttt ttcttttatt gtacattgtt ttactcattt aaataaatct tataaataaa
     1141 tcttcataca aataacacca atgtgcatag ctgctaacag cacagcactc aataagtgtc
     1201 cgaagtatag ataattctta tcttacttaa gtagcttaca aaattaataa ctttttgtat
     1261 aatgtttaat gctgcattaa tcaagaaagt tgccaatgtt cctaacatcc caatgcctta
     1321 atcagcattc tgtatgccga atccattgcc tcttccagac atgtagtggc ataccagtcc
     1381 atgtgactaa tgtatcctga aaaggccgcg acagttcccc gtactaccat tgcctggtag
     1441 tacggagttt cattattatc aatcagccct tcacttattc ccgtctgtaa taaatgctct
     1501 atcgcgcaat taaatatttc cataagctct gaaaaatcgc attcataatc taccagatac
     1561 tcataaaatt cctgaacact tcctgtagct attagcatat tgaaagtaga tttgttattt
     1621 tccatacttt ctgccataat atgcaaaatt tcgtagaatt tatctctgaa cttttctgtg
     1681 gattttattc tcgtatatgc atattcagct tccttattta tgctatatag tattgctctt
     1741 gaaatagctt cttcctttga tttaaagtag tcgtaaattg tacccttccc tacttctgaa
     1801 gcttcagcaa tatctgatac tttaatggaa taaggattcg ctccatttct tattaattca
     1861 atcaacccat taaatattgc aatttccttt tccgagtatc cattattgcc catatattat
     1921 acatctccct cattcaatac ctttggcatt ttatttttat gaagtgaatc atacattatg
     1981 ggaactacaa aaagtgtaag aactgttgca tagattaacc caccaataga tacaatggcc
     2041 agaggctgca gcatttcaga acctacacca actccggcgg ataatgtaga caatcctaaa
     2101 atggttgtaa ttgcagtcat taatataggt ctcatacggg ttcttcctgt tgatactaaa
     2161 gcttcttttg tgctcagtcc tttatctcta agctggttgg tatagtcaac aaatactatt
     2221 ccattattca ccactactcc actcaataca aggaatccca gcattgcaat taagcttata
     2281 ttatttccgg taatcgccaa agccaataat ccgccggtaa aagcaagagg aatggtaaac
     2341 ataactataa acggtgacag cagggattga aattgtgcca ccattataag gtagatcaat
     2401 gctgctgcca atagcagcat ataagttaaa tctctcagag aatcgttgat cattatattt
     2461 tctccagcaa attcaattct atatccatcc ggcaatttgt agtccttaag cttgttttcc
     2521 aggtcacggc ttacaagacc gatattatgt tgtgcatcaa tggcggcagt tacagatata
     2581 tacctctctt gtgactttct tctaatagat gataatcctt tctcctctgt aatagcagct
     2641 atatcaccta atctaacctc tgtttccaca ccatttttat tagattttat tttcagatct
     2701 tctaaatctt ttctcataat agactgattt tcgccatcta taactattac cggataatct
     2761 ttggatatac cggatatagt ggtagctgat ttacctcttg aaataccgga atttacactt
     2821 gagaaaactt gagctactgt tagtcctttc tccattgatt tttccttatc tactgtaatt
     2881 cttatttcag ttgagttctc atctgttccg gaagcaactt ccatagtgcc ttcagtttcc
     2941 ccaaggattt tagatatatc cagggcaata tcacgtaaag tatccagttc ccgcccttta
     3001 acaatcactt ctattccaga tccgcctaat gctgacatat ccatgttgga ggagtctata
     3061 gcaatctctg cattaagccc atcggtcaat ctctttattt ctttttcaat ttcagcgttg
     3121 cttacaccct tattctcatc aagaattaaa tacattgaca ttgtattacc gccggctctt
     3181 ccactggatg aaccaaaaat acccatgccc atgccttgat tttgaaatgc gcctatagtt
     3241 ttaattcctt caatacccat aatcctatct attactgtgt cagacatagc aatggtatct
     3301 tcatatgtgc tttcctttgg cattttcaag gttatagaca tttgaggagc ttccatatcc
     3361 ggaataaatg atgtgcccat agatgctgca tagtatgcac tgagtaccag taaggcaaca
     3421 acagcaatca taattacagc cttgtacttt aatgaccagc gcagcatttt ttcatagcca
     3481 tctaaaaatg tatcatatat attacgcgtt ctattttcag tacttttgag catgcttgat
     3541 gccatagccg gtactaaggt aagtgctaca ataagacttg cgaataatga gtaggctata
     3601 gttaacccca tgtcggtaaa taattgcttt gatatacctt taacaaatac aattggtaaa
     3661 aacacacata ctgttgttaa agtggatgaa atgatagccc ccgacacttg cttggctcct
     3721 tctatagagg cagctgaagc agattcaccc tcacttctca gacggtatat gttttcaatt
     3781 acaactattg agttgtcaac caacatacct actccaagag ccaaccctga taaggaaata
     3841 atatttatgt taaccccggt aaagtacatc atagctacag cgaaaacaat acttattggt
     3901 atggataaag ctattattac tgtcggacgg gtatctctca aaaagactaa taatataagt
     3961 attgctaaca acccgccata cattatattc tttaatactg aagccacaac aatatcgatg
     4021 tatactccct gatccattaa tgcagtaaaa ctaaaaccat cattttcaga ttctatttta
     4081 ttcgctcttt ctcttatact ttttgcaact tcagcagtag aaaaaccgct ctgcttttgg
     4141 aatgtaagca ttacagcatc atttccattt acttttgcat atatatcttt tgaattgtcc
     4201 ttcatttgga tatctgcaac atcgctaaga taaacctttc ctactccctc aactcccgta
     4261 tcaaataaaa gcagcttttt tatttcatcc atatctttta ttttattacc aactttgact
     4321 aaataatctg taccctcttc tttaacatag ccagcaggca ttgaaaagtt ttgagctgca
     4381 agaattcctg aaatcatgtt ggtagaaata acaccatcca gggaagctct tttgaaagca
     4441 gcttctttac ttatttccag ctcttttaag ttttcattga ttgttttttc agtgtcaatt
     4501 agctgtgttc ttgctttgct catctcacca gctaataaca ctttaccctc atcaatttgt
     4561 tctttttttt catttagccc ttctctttgc ttaactaata aagccttatc cttttcaagc
     4621 ccttcaatct tattggataa ttcattgata gcagcagcca tttcagcctt tttgcctgaa
     4681 atatccatta agcccttttg aatgccagct aactttactt tatcttcttc acttatattt
     4741 ttccctaaag ctaataatgc tttttcaacg gctattaatt cttgctcatt tttatttatt
     4801 tcctgcaatg cactttccag atcattttta tttttaatta attctagctt gccagtctcc
     4861 aaacctttct ctgctgcaga aatttgttca aaggcttttg ttaattcttt ttcaccttca
     4921 gctatttttt cactttcact tttatttctt gattctaatg tagctttacc ttcatctacc
     4981 tctcttttag ctttcagaag tttgtcttca gcttccgcca tcttagagtc aatgctgtcc
     5041 aatatttttt tattaagatt atctattttc ccttcactaa tggttacttc aatattttct
     5101 tccagaagtc ccacacctct aacagatgct acaccattaa cactttccaa ttcaggaata
     5161 accttctcac ttaccatttt agatatctca gctacattca taccttcaac atcagcagaa
     5221 gcaaccatta ttggaagcat atcagggttt atccgtatta tcataggcaa cctaacagaa
     5281 tcactccatg ctccttttat taaatcaagg ttgctgttaa tttctattat tgctgagtcc
     5341 atattaacgt cattgttgaa ctccaatatc acaacagacg agttttccct ggaaatagaa
     5401 cttacatttt ttatattatt agtccttgcg acaacctgtt caataggcct tgtcactact
     5461 aactctacct cttcaggact tgcacccggg tatgaagtag ttattacaac atacggcagg
     5521 tcaatattgg ggagtaaatc agtttgcata ttgataaatg aaataactcc taatataagt
     5581 accacaataa cagctacagc tattgtgtaa ggtctcttta cactgaattt cgataacata
     5641 aatcaaatgt cctcctaagt aatctaattc cgaccgacta gtcggtcatt tctatattat
     5701 agaaaattag atttaccttg tcaaggttct gttaaaatac atccacattg ttatatcaca
     5761 tcattacacc ataaatcata tccctggtta taagcaattt gaaacaacgg aataatattt
     5821 gattttatgg aatctgagaa ttcatgtttt gaaatattat tgtataataa agaatttaaa
     5881 tccagctttt tattaattgt aaggtaatct tccaggtaat tcctaaaggc tttattcttc
     5941 cttattatat taaaagcact atattgaata tcacagaacc tatcaatata cagatcgacc
     6001 tttggcaatt tattgctctt agagctgtta atattcctaa aagtaggtac aagattccac
     6061 aattcatcat gaaggacaaa actccatggt ataaagtggt caatgctgat tcctccatac
     6121 tgttcaaaat tatctgacgt taaaggttta tttgtatata tatcattgat gcctatatgt
     6181 ttcataacct cgctccaata tttttgggcc gcatttaaat ctctctgata tggtggtgat
     6241 aatttaaacg gtatagcagg tacattgggg ttctttttct gaagataata tatcattttg
     6301 tagttaagcc atcccgcaac tatattctga tttctataaa tatattcaaa ccagttttca
     6361 tttaccagaa tagctttatc cggcataata atctggtata gtgctttagg cgagctatga
     6421 gataaatcca gtattacctt gttcttttct gcgtccttga ggcccgccaa tacttcggaa
     6481 taaaatggtg atataagcct atagggtaca tacctgtaga attcaattat tttttgctca
     6541 atttccttat cctccatatt gtccaggaat aacaataatt cttcttcttt gacatcactg
     6601 ctaataccgt acttcttata tatcattgca actatttcat ttagcttatc gctaaccccg
     6661 aagttaagat gatattgcaa aagcggatac caagcagtag ctatcattct gcagacaact
     6721 cttctaaatg atatttgcct attcccacga acaatttcct taaacatccc ggaaaaccaa
     6781 aaaattttat aacttgttgt taaatattca tactctaata actgctcaag tatcctcaca
     6841 tctacagtat aatcctgagg taatttaaac attgttcccc accttcaatc taaagataac
     6901 atctatattc tatctcaagt atacctgatt acacctcttg acatcaatat ttatctaatt
     6961 tataattata ttagctaata taaaatatta gctaatataa ttatggttaa tgggagatga
     7021 tgccatggaa gtaccatcta ctgaagtaca gaataatttt gggcaataca tgaaatttgc
     7081 tttttatgaa gatataattg taacacggaa tgggaaaagg gctgtagtta ttaagcgtta
     7141 tgaagaggac aatgaaaaag gcattaaagg aactatatta gctgaaaagg cccaagcata
     7201 caatttagag gatcttaaaa tatcatacga ggattttatt aaacttactg aagaaagtga
     7261 taacaggtat gaatatattg atggtgaagt gtacctgctt gcttctcctt catataatca
     7321 tcagagtatt attgtagaaa tattaaatac catgtataac cggtttaaag gtgagaaatg
     7381 caaaccgttg actgcaccgt ttgatgtaac cctattaaaa gaagataata aaaatgtagt
     7441 acagcctgat attttagtta tctgtgatac ggtaaatatt aatgaaaaag gaagatatac
     7501 aggaatacca agtcttgtat tggaagtatt atctgaaaca acaaagcata aggatttgct
     7561 taaaaagctt gatttatata ttcacagtgg aataagcgaa tattggattg taaatccttt
     7621 caaaaaggaa atttatgttt attacattga ggataaagca atttctgaat atgaagtata
     7681 taaaggtaat gaaggagcaa aatccatact gtttaaaggc ctggaaatat cccttgagca
     7741 aatatttgag ttttaatgag gtacaccaaa gagcaaattt atagttgcaa tattgaaaaa
     7801 taaaagttct aacccgagag gtacatttgg gtgcatttgt attattatat tatcattcta
     7861 ttttgtggca atattatcat tgaactacaa atttaactat aagcagcaga aatacatgaa
     7921 atacatattt aacttcagga tgaaaaataa aaatttccca ggaagaaagg aatgtggtgt
     7981 ttatggcagg caagcaggaa aacaattatc aatatgcaat atttgcaggt ggctgctttt
     8041 ggtgtatggt tccacctttt caaagtatgg atggggtagt ggaggtaata gccggatata
     8101 taggaatagc aggctatgca aaactcaaat ttacatgaat tggcctccac ttattacgca
     8161 aaacagaggt ggaaagagtt tttacaccgc cagctgtccc tttaaaaaag ctatatattc
     8221 tctacaaact gaatttttaa tccattcggg tctaatacat agaaaaactt gatatgcgga
     8281 tttggttgaa acggtccact gtgcacagct atgccttttt ctttaacaaa tgtcatcatt
     8341 tcatcaacag aatgaacttc aaaccccagg gaaatgtcct gccctaatcg tagtccaaca
     8401 gaatttcata aatcattcca ccttccttaa aaatcgtttt gcttttacaa taattttata
     8461 tccaaatttc atccgcttac aactaatttt tgctattcct cttcaataaa ttttttgctt
     8521 ctttttgata tcttgggata cttaactcca ttttccggct gctctatccg cttgcactca
     8581 atttcaaact tattttcatc tccataatca tataaatatg caaactttga tccctctttg
     8641 agattcaatg agtcaattct aatcttgtca gcctttctct ttccatcagc ttggggatca
     8701 tgatattcag aggcattgtc ccagaactta ccactcataa aaaaagaata aagatgtccc
     8761 caatcaagat taaagttact ataaataacc tcatgaaggt catcaagtgt actttctcct
     8821 agtatatcta cttgaatcca gattgttttt gcatggaagt tttttacctt aaacacatat
     8881 agatttcctt tactctttgg ttcccgccct atttcaggtc cattgatatc tcttcttttt
     8941 ctat
//
LOCUS       DAOSDD010000191         8856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2922_length_8856_cov_5.364731, whole genome shotgun sequence.
ACCESSION   DAOSDD010000191 DAOSDD010000000
VERSION     DAOSDD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2922_length_8856_cov_5.364731"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK733_14620"
     CDS             <1..546
                     /locus_tag="PK733_14620"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOJ11809.1"
                     /translation="FADSSLSGSPNDSANGNLLVFFGGDKEVYDRWLPLMRTFSREVK
                     YVGASGSGNIAKLACNYLGVMYATLYAEIFPLLEKLGVDINQFYDIIGSTKVDCGVYR
                     FYAPKIVNKTYDMGFSLELALKDITYVKRMFEEYEVPAFILEGGLNLLRTSVKDGKAN
                     KDLSECAATVRDYLKILGGMQ"
     gene            780..1490
                     /locus_tag="PK733_14625"
     CDS             780..1490
                     /locus_tag="PK733_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11810.1"
                     /translation="MKSANSKKRIPWKALTAVILFTSLALSIIFTVVKVITTPEEIVN
                     PEIQTRVKSDYVLMLIQCILGLVVMMIPSLVERKWSIIIPNNIQIMYFLFLYCAIYLG
                     EVKDFYYLVPHWDTVLHAFSGAMLGLLGFALVSFLNDSKHVKFALSPFFVSLFAFCFA
                     LSAGAIWEIYEFTFDSLLSLNMQKFALSDGTLLVGQEALADTMKDIIVDAISALAVSV
                     FGYFSLGKKEEIKAGIGL"
     gene            complement(1505..2479)
                     /locus_tag="PK733_14630"
     CDS             complement(1505..2479)
                     /locus_tag="PK733_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ11811.1"
                     /translation="MNIISKENAKAFLFFNAFLWGSSYVWSKMLLSYLPHFMILFLSS
                     LGGLITITILYYKKIKTIKKQSLLRCIFISSLSIISNTFFMFALQYTDSYNAAFIVQL
                     SVVITPLIITCFKKRLPEGRVVVSAVGALAGIFLLNFDAKGFQFNIGDLLALGNAVFF
                     SLYLASLKINSKRIDPVQFTFIQHAVNTPVYLLLAFLLETGNIRLENMKSAPLIILIT
                     VSIFITVFTILIQSSAIKYVQAEKAVLIYTIEPVTAMILGLFFLGEKPEGFGAITGCI
                     LIIFSIMISLYEPQYQQEKHYKGEMQNRGEKRKKEGSRAMSRVFVEKL"
     gene            2760..3683
                     /locus_tag="PK733_14635"
     CDS             2760..3683
                     /locus_tag="PK733_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11812.1"
                     /translation="MQTLITNIHSSKRGYLNRDFEFFHLKDQKSIHFEYHHHDFVKII
                     IFISGKVTYLIEGKAYRLKPWDILVIGNEEIHKPIIDSSEFYERIVIWINPAFIEKHN
                     SSDCDLMTCFKLASKEKYYLLRPDAESLRNTRYILSQLELENSDKNNLFGWHILKNSI
                     LMQLVVYLNREYLYKGSAAKADLSDSDDLKYDENIEKILDYISSNLDSDLSIDNLSSQ
                     FYISKYYLMHMFKKQTGYSVYQYILYKRLILAGSLIKKGKSASQVCIECGFGDYSNFL
                     RAFKKMYGIPPKEYYKSCLERKKEQSLHHFD"
     gene            complement(3749..4420)
                     /locus_tag="PK733_14640"
     CDS             complement(3749..4420)
                     /locus_tag="PK733_14640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ11813.1"
                     /translation="MKERIYKFAPVVLLFICLIVFTIIFIIIKHLHGYSHSFIFAVLL
                     ICQMINSLIYGVSIKKLYRQVQIDALTGLFNRRCFNEKLYKMKSRGSTCLILIDIDNF
                     KNINDTYGHLMGDQVLQQLAQILQNSTRKKDIIARWGGEEFAIILPETSIEDAYMIGN
                     RIRTKVENYIFSYEEVICNITVSIGIAAANTSKDIDPDQFLKITDEALYDAKKKKNNI
                     SAVMG"
     gene            4579..4989
                     /locus_tag="PK733_14645"
     CDS             4579..4989
                     /locus_tag="PK733_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11814.1"
                     /translation="MEVNNREIGQRIRNEREKLNLTREKFAEIIGLSDYYIGQLERGE
                     RQMSLPAMVNIANCLHISLDYLIFGETEYNNYCIHESDNNYGNLDSLNSGKCAEMNEL
                     TGMNELLELIGKCSPKELDLIKKLIKTILPYMVS"
     gene            complement(5099..5443)
                     /locus_tag="PK733_14650"
     CDS             complement(5099..5443)
                     /locus_tag="PK733_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11815.1"
                     /translation="MKLFMGDNCVSCGKAQNGSSSNIMENRVKSCFFSLPPKQFSLLA
                     TLIGILLIDNLDANQQNSLGNFIVSIGQGILTAVSQQALLESNEEKNDHIKQQIKMLK
                     KQICSLEDELNS"
     gene            complement(5572..6507)
                     /locus_tag="PK733_14655"
     CDS             complement(5572..6507)
                     /locus_tag="PK733_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOJ11816.1"
                     /translation="MKRLLMVFIIISGLLTSCISQNTDKTNKTNKTNNTSQKGITRSV
                     QDQGQDKGKLLEKGKLGLINENGDTIQERINVPEGFERVEVEEDSYEQYLRSLPLKPH
                     GSKVKYYDGKTKNKDVHVAVVDMDVGDRDLQQCADAVIRLRAEYLYGRKLYEKIHFNF
                     TNGFLADYPTWMSGDRIVVEGNKVYWAKKTSYSKDYSSFRKYLDMVFAYAGTLSLSVE
                     MKKIPLDDMQIGDVFLKGEDPGHTVIIVDMAVNDSTGEKLFVIAQSYMPAQDIHVLKN
                     PEDEELSPWYSVNFGETLNTPEWRFNRDQLMRFPD"
     gene            complement(6675..7193)
                     /locus_tag="PK733_14660"
     CDS             complement(6675..7193)
                     /locus_tag="PK733_14660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ11817.1"
                     /translation="MKNPLVTIEMENGNKIKVELYPEVAPNTVRNFISLVESGFYNGV
                     IFHRVIPGFMVQGGDPEGSGMGGPGYSIKGEFSRNGFTNNLKHDRSVISMARSSNPNS
                     AGSQFFIMVEKAPHLDGQYASFGMVIEGMEEVDRIVSVRRNHSDKPNEDQRMKIVTVD
                     TFGENYGEVEKV"
     gene            complement(7333..7698)
                     /locus_tag="PK733_14665"
     CDS             complement(7333..7698)
                     /locus_tag="PK733_14665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11818.1"
                     /translation="MLVHSGPKDIAEKVEYLSKMYPGISFLLAHSGGSYITSGYNIEV
                     ARKRDNVYLEITYTSLTNGMIEFMVDEAGADKVIFGTDMPMRDPAPQLAWVCYARISV
                     EDKRKILGLNIKKLIDRCQ"
     gene            complement(7855..>8856)
                     /locus_tag="PK733_14670"
     CDS             complement(7855..>8856)
                     /locus_tag="PK733_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11819.1"
                     /translation="LINYIIDPYQFYRKPVLYKPVYFNDQRYQNPGLARSQDYSLAIV
                     GTSMTDNFLPSYVENKLDSKTVKLSMMGASIYEEKLMVDVVSHAKKANTVILELNYFS
                     FRGDTGRVTETGKGFPHYMYKNGLMSDLKYLCNPETLLQSYHILLRNYKGKMHYTMSE
                     DLEKLNTWYYNSKFEKANAMKDWNLAQNDLKRADPAEFKWEYLRANADKNLVEVIKRN
                     PDVEFILFYPPFSILAHKFLDSRNLFDDEIRLKRYIYARCKDFRNVKIFDFQDIEGIT
                     HNLDNYRDMTHYSIEINEYIIDSIARGENAITEDNMEEKLKNLAEQVESYVVESARS"
BASE COUNT         2860 a         1569 c         1421 g         3006 t
ORIGIN      
        1 tttgcagatt catcactttc cggttctcca aatgattcag ctaatggaaa tctgctggta
       61 tttttcggcg gggacaaaga agtatatgat agatggcttc cattaatgcg gacttttagc
      121 agagaagtaa aatatgtagg cgcttcaggg tctggaaata ttgcaaagtt ggcttgcaat
      181 tatcttggtg ttatgtatgc aacattatat gcggaaatat ttcctttgct agaaaaacta
      241 ggtgtagata taaaccagtt ttatgatatt attggcagta caaaagtaga ttgtggagtg
      301 tacagatttt acgcaccaaa gatagttaat aaaacttatg atatggggtt ttcacttgaa
      361 cttgctctta aggatataac atatgtaaaa agaatgtttg aggaatatga ggtaccagca
      421 tttatattag aaggtggact caaccttttg aggacttcag taaaagatgg aaaagcgaat
      481 aaagacctga gtgaatgtgc agcaacagta agggattatc ttaaaatatt agggggaatg
      541 cagtagtaga agagattaat atacacaggt tctcaaattc tacatgggaa taaaacttga
      601 taactaaaaa agtacgacct gtgtaggtaa ttacaccata aatattatat gtagtaatcg
      661 attattgcca tatttttctt caaatatagg aaatgcacca atttcccata aaatcgcttt
      721 acaattaagt atattttatg gtaagttata gtaaacagta ttatttgagg ttatttatta
      781 tgaaaagtgc aaattcaaaa aaaagaatac cttggaaagc cctaacagcg gtaatactat
      841 ttacaagcct tgcattatct attattttta ctgttgttaa agttattact acacctgaag
      901 aaatcgtgaa ccctgaaatc cagacaaggg taaaaagtga ttatgtcctg atgcttatcc
      961 aatgcatatt aggtttagtt gttatgatga ttccttcgct agtagagaga aagtggtcta
     1021 ttataattcc aaataacata caaataatgt attttttatt tttatactgc gcaatatacc
     1081 tgggcgaagt aaaagatttc tattacttgg tacctcattg ggatacagta ttacatgcat
     1141 ttagtggtgc tatgttaggg ctgttaggat ttgctttggt aagcttcctt aacgattcga
     1201 agcacgttaa atttgcttta agcccatttt ttgtatctct atttgctttt tgttttgcat
     1261 tgtccgcagg tgcaatatgg gaaatatatg aattcacttt tgattcactg ctatcactaa
     1321 atatgcaaaa gtttgcatta agtgatggga ctctgctagt aggtcaagaa gcattagctg
     1381 atactatgaa agatattata gtagatgcaa taagtgccct ggcagtttcg gtttttggct
     1441 atttttctct gggcaaaaag gaagagataa aagcaggcat tggcctgtag agtgaagtaa
     1501 atttttataa tttttctaca aaaacccttg acatggcacg gcttccctct tttttccgct
     1561 tttcacccct attttgcatt tcacccttat aatgcttttc ctgttgatat tgcggttcat
     1621 ataaggagat cattatagaa aatattataa gaatacagcc tgtaattgct ccaaatcctt
     1681 caggtttctc accaagaaag aaaagtccca atatcattgc tgttaccggt tcaatggtat
     1741 atataagaac tgccttttct gcctgaacat attttatggc gctgctttga ataagtatag
     1801 taaatacagt aataaaaatg ctgacagtta ttaatattat taatggagca gatttcatgt
     1861 tctccaatct gatattgcca gtttccaata aaaaagccaa aagcaggtat actggagtat
     1921 tcactgcgtg ctgaataaat gtaaattgaa caggatctat cctttttgaa tttattttaa
     1981 ggctggcaag gtatagagag aagaatactg cattgccaag agcaagaagg tcaccaatat
     2041 taaactgaaa ccctttagcg tcaaagttta aaagaaatat tccagccagt gctcctactg
     2101 cagacactac aacccttccc tcaggcaaac gttttttgaa acaggttatt atcaaaggtg
     2161 ttattaccac agatagttgt acaataaatg ctgcattata actgtctgta tactgaagag
     2221 caaacataaa aaatgtattg cttatgatag ataaggaact tataaaaatg cagcgcagta
     2281 aagattgctt ctttattgtt tttatcttct tataatacaa aattgtaatg gtaataagtc
     2341 ctccaagaga acttaaaaac aatatcataa aatgaggcag gtatgatagc aacattttag
     2401 accatacata ggacgagccc cataaaaatg cattaaaaaa taggaaagcc ttcgcgtttt
     2461 ctttactaat aatattcata ttgatctcct ttatgtgtta atattttgta cttatctttt
     2521 tattttcgca gaaccaggtt aaaacctgca tttatcatac ataatagtat attcaaacta
     2581 tttgctatgt tttgcgctta taatgcttga aatattgcaa aagctgcttt aaaatataat
     2641 agagagatga aatatcatgt gagaacatat tattatgcac atattgcaat gcacattata
     2701 taattcacat aggataagtc gaatgagatt agtaataaat tatggaaggt gaacatataa
     2761 tgcaaacttt aattacaaat atacatagtt caaagcgtgg atatctaaac agggattttg
     2821 agttttttca tctaaaagat caaaaaagta tacactttga atatcatcat catgactttg
     2881 tgaagattat tattttcatt tccggtaaag tcacctacct catagaaggt aaggcttaca
     2941 gacttaagcc atgggatatt ttagtgatag gtaatgagga aatccataaa cctattatag
     3001 actcctcaga attttatgaa agaattgtta tttggattaa tccggccttt atagaaaaac
     3061 ataacagttc tgactgcgat cttatgactt gctttaagct cgcatcaaaa gaaaaatatt
     3121 atttattaag accggatgca gaatctttaa gaaacaccag gtatattctt tcacagcttg
     3181 aattggagaa cagtgataaa aataaccttt tcggatggca tatattaaaa aactccatcc
     3241 tgatgcagct tgttgtatat cttaacaggg aatatctgta taaaggaagt gcagcaaagg
     3301 cggatctttc tgactcagat gatcttaagt atgatgaaaa catagagaaa atactcgatt
     3361 atattagcag taatcttgac agcgatttat caattgataa tctatcatca caattttata
     3421 taagtaaata ttatcttatg catatgttca aaaaacaaac aggttattct gtatatcaat
     3481 acatacttta taaaaggctt atactggcag gcagccttat taagaaaggg aaatcggcaa
     3541 gtcaggtgtg tattgaatgc ggttttggag attattcaaa ttttcttcgg gcatttaaaa
     3601 aaatgtatgg tataccccct aaagagtatt ataaaagctg tctcgaaagg aagaaagagc
     3661 aaagcttgca ccattttgat tagggtccgc attataaaat atgaagaaac atttaccagg
     3721 gctgtggtta cggtgacaaa tatcctgact accccataac cgcactaata ttgttcttct
     3781 ttttctttgc atcataaaga gcttcatcag taatttttag gaattggtca ggatcaatat
     3841 ccttgcttgt atttgcagca gctatgccaa tactcacagt aatattacat ataacttctt
     3901 cataagaaaa aatatagttt tccacttttg tccttattct gtttcctatc atataagcat
     3961 cttcaatact ggtttcagga agaataatgg caaattcctc cccaccccac cttgcaatta
     4021 tatctttttt tcttgtgctg ttttgaagaa tttgtgccaa ttgctgtaat acttgatcgc
     4081 ccatcagatg tccatatgta tcatttatat tcttaaagtt atcaatgtct ataaggatta
     4141 gacaagttga gcctcttgat ttcattttat ataatttttc attaaagcac ctcctgttaa
     4201 atagacctgt gagtgcatca atctgtacct gtcggtataa ttttttaatt gaaactccgt
     4261 aaattaagct gtttatcatt tggcaaatta aaagcactgc aaaaataaag ctatgcgagt
     4321 agccatgtaa atgtttaatt attataaata taatagtaaa tacaattaag catatgaaaa
     4381 gtaaaactac aggagcaaat ttataaattc tttctttcaa tattatcacc ttccagcaaa
     4441 ttataacttt caatattata tattaaatgt atgccctttg caagtatttt ttgtgtgccc
     4501 tttgcatatt ctattattat aaaaagttgg ataaagtaat tatgtgtagg gaacaaatct
     4561 aagccggggg taagtatatt ggaagttaat aatagggaaa taggacagag gataagaaat
     4621 gaaagagaaa aactgaatct gacccgtgaa aaatttgcag aaatcatagg tctttccgat
     4681 tattatattg gacagcttga acgtggagaa aggcaaatga gcctgcccgc tatggtaaat
     4741 atagctaatt gcctgcatat atctttggat tacctgattt ttggggaaac tgaatataat
     4801 aattattgca ttcatgaatc tgataataac tatggcaact tggatagctt aaattcaggc
     4861 aaatgcgcag agatgaatga attgacaggg atgaatgaat tgttggagtt aataggaaag
     4921 tgttctccaa aagagctgga tttgataaag aaactaataa aaactatcct cccttatatg
     4981 gtaagctaag ctaagacatt gtcttgcata aaatgccaat accaaaaatt aatttgtata
     5041 atattaaatt tattattgcc ttccgaatat atcccattat attttatcgt tgatgccgtt
     5101 aactgtttaa ctcatcctca agtgaacaaa tttgcttttt cagcattttt atttgctgct
     5161 ttatatgatc atttttctct tcattacttt ctaataatgc ttgttgagaa actgctgtca
     5221 aaatcccttg tcctatactt acgataaagt tgccaaggga gttctgctga tttgcatcca
     5281 gattatcaat caatagaata ccaataagtg tcgccaacag cgaaaattgt tttggtggga
     5341 gagaaaaaaa acaggatttt accctattct ccataatatt tgaacttgaa ccattttgtg
     5401 ccttgccaca ggaaacacaa ttatcaccca taaacaattt cacttctcct cttcccaaag
     5461 agtattatac aatttatttt tatatatacc ctatttgcag gtatactcca tttataaata
     5521 ttatagttca ttatatgggt tcaaactatg aatagtgcaa taaatatgaa tctaatccgg
     5581 gaatctcatt aattgatccc tattaaatct ccactccggg gtattaaggg tttcaccgaa
     5641 attaacagag taccagggac ttaattcctc atcctcaggg tttttaagta catgaatatc
     5701 ctgcgctggc atgtagcttt gagcaatgac aaatagtttt tcccctgttg aatcattgac
     5761 agccatgtcg actataatca cagtatgccc gggatcttct cctttcagaa atacatctcc
     5821 tatttgcatg tcatctaacg gtattttctt catctccaca gaaagggaca atgtacctgc
     5881 ataagcaaaa accatgtcaa gatattttct gaaactacta taatcttttg aatatgatgt
     5941 tttctttgcc caataaactt tgtttccttc cactacaatc ctgtcgccgc tcatccatgt
     6001 aggataatct gcaagaaacc cattggtaaa attaaaatgt attttttcgt atagtttcct
     6061 gccatataag tactcggctc ttaatctaat aacagcatca gcgcactgct gtaaatccct
     6121 gtcacccaca tccatatcta ctactgctac atgtacatct ttgtttttag ttttcccatc
     6181 ataatattta acttttgagc catggggttt taaaggaaga ctccttagat attgctcata
     6241 ggagtcttcc tctacctcta ctctttcgaa gccttcaggt acatttattc tttcctgtat
     6301 agtgtcacca ttttcattta ttaaacctaa ttttcccttt tctaaaagct ttcctttatc
     6361 ttgcccctga tcttgaacag acctggtaat acccttttgg gaagtattat tagttttatt
     6421 ggttttatta gttttatcag tattttgact tatacaagaa gtcaaaagtc cagatattat
     6481 aatgaacacc attaagagcc ttttcacatc agtccaccct ttattatttg taaatttgca
     6541 agctatagat aacgcacaaa agaaactgca ttataaaagt tttgaacgct tatgttacct
     6601 taattaatac gcaaaaatcc aatttgttcc ttgtaaaatc atttttgcgt tatatatagt
     6661 aagtttgcac ctgttcatac cttttcaact tccccataat tctcaccaaa agtatcaact
     6721 gtaacaattt tcatacgctg gtcttcatta ggcttatcgc tgtggtttct tcttaccgat
     6781 actattctgt ctacttcctc cataccttca ataaccatac caaaagaagc atattgtcca
     6841 tcaagatgcg gagccttctc aaccattata aagaactgag aacctgcaga atttggattt
     6901 gaagaacgtg ccatggaaat aacacttctg tcatgtttta agttattagt aaatccattt
     6961 ctggaaaact caccttttat ggaatatccc gggccgccca ttccagaacc ttcagggtct
     7021 cctccttgta ccataaatcc tggtataacc cgatggaaaa taactccgtt ataaaagcca
     7081 ctttcaacca atgaaataaa gtttctaaca gtattaggag ctacctcagg atatagctca
     7141 acttttattt tattaccatt ttccatttca attgttacta aaggattctt cattatgaat
     7201 ctacctctca atctttttag tattataaaa caattctacc acaaaaattg agatattaat
     7261 ccaaaaggtg gttaaatcta ctgaaggtgt attaaattta tatgaacaaa atgcacccat
     7321 ccaccgaatt tattattgac acctatcaat caacttttta atattaagtc caagtatctt
     7381 tctcttatct tccacagata tccttgcata acaaacccag gcaagctgag gtgcaggatc
     7441 tctcatgggc atatcagttc cgaatataac tttatctgct cctgcctcat caaccataaa
     7501 ttcaatcatt ccattagtaa gagatgtata tgtaatctcc aagtatacat tatccctctt
     7561 ccttgctact tcaatattat atcctgatgt aatataagag cctcccgaat gagcaaggag
     7621 gaatgatatt cccggataca ttttgctcaa atattccact ttttctgcaa tatcttttgg
     7681 cccggaatga acaagcataa tcaagttttt tctatcacca tattcaaacc atttccgata
     7741 cttttctcca agcaagtcat acttgttaga acagtagtat ggcttaattc ctatttattt
     7801 aataatacct tttcttcagg tatttttaaa gcactgcaat ttgtggcaaa tgaattagct
     7861 cctcgcagat tcaaccacat agctctccac ctgttctgcc aggttcttaa gcttctcctc
     7921 catattgtcc tctgtaatag cattctctcc ccttgctatg gaatcaatta tatactcatt
     7981 tatctctatt gaataatggg tcatatctct gtaattatca aggttatgag ttatgccttc
     8041 tatgtcctga aaatcaaata ttttcacatt tctgaaatct ttgcatctgg cataaatata
     8101 tcttttcagc cttatttcat catcaaataa attcctgctg tcaaggaact tatgagcaag
     8161 gattgaaaag ggcggataga acagtataaa ctcaacatct ggatttcttt ttattacctc
     8221 taccaggttt ttatctgcat ttgccctaag gtattcccat ttgaattcag cagggtcagc
     8281 cctctttaaa tcattttggg ctagattcca atctttcatt gcatttgcct tttcaaattt
     8341 cgagttgtaa taccatgtgt tcagcttctc cagatcttcg ctcatagtat aatgcatttt
     8401 gcctttgtag ttgcgaagaa gaatatgata actttgcaga agggtttcag ggttgcaaag
     8461 atattttaag tctgacatga ggccattttt atacatatag tggggaaagc ctttccctgt
     8521 ctcggttacc cttccggtat cacctctgaa agaaaaataa ttcagctcca gaataacagt
     8581 attagctttt ttagcatgtg aaacaacatc taccataagc ttttcttcat atatggaagc
     8641 tcccatcatt gaaagcttta ctgtcttact gtccagctta ttctcaacat atgacggcag
     8701 gaaattatct gtcatgcttg tgcctacaat agcaaggctg taatcctgac tcctggcaag
     8761 cccgggattt tgataccttt ggtcgttgaa ataaaccggc ttgtacaata caggttttct
     8821 ataaaactga tatggatcta ttatataatt tataag
//
LOCUS       DAOSDD010000192         8834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2929_length_8834_cov_5.075407, whole genome shotgun sequence.
ACCESSION   DAOSDD010000192 DAOSDD010000000
VERSION     DAOSDD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2929_length_8834_cov_5.075407"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..827
                     /locus_tag="PK733_14675"
     CDS             429..827
                     /locus_tag="PK733_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ11820.1"
                     /translation="MDKSKSSRISDAEWQVMNVIWDTDRTTSQAIVKQLKAKTNWSEM
                     TIKTLIGRLVKKKVAGYEVDKNDKRIYYYYPIIKRDQCIRDETNIFLKKFYNGALSSM
                     LSSFVKKNELTREEMDELIKILESGKKKEE"
     gene            833..3457
                     /locus_tag="PK733_14680"
     CDS             833..3457
                     /locus_tag="PK733_14680"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11821.1"
                     /translation="MYSEFFNWLIQSSLKASLIILIVLFLRKFFEDKINARWYMILWT
                     IVTIRLILPWAPQTNTSMFNVLDLSVNVIQSAIITGENIENEIEKSKASINKSEHNKS
                     EYFENHSEQDVDTYYFDISPDINSDTQPYEASAINKTFDSRLNVLGHDISHNPQTPPL
                     LYLILIFWIVGLLAFYSYIVLINIKFYRKIKDKKPITDERIYYITEQCRKQLCIKIPI
                     TLFETGAVEGPSIYGIFNPIILIPKNMLSILSDSEIRCILIHEFMHYRRKDNIFNLFT
                     MVISGLHWFNPLVHIAFAMMRHDSELLCDSRVLKHISGEEYIYGKTLIKLACNFAGNS
                     LIISEGMGGNGKGLKERIKRIAGTARYKAGWSIIAILMVIIVGCTGLSNEKAPYEPDI
                     FYNLEPVNLNFYTQGYNGSTAEIGDMLKEIEQKLTGSLRITPVFHWIPYEEYDDKLKT
                     LITSGEAVDAFTSYTPRTYVQEDVALDISALFPQYAPEYYEQLKSNEAGEMLLESVTV
                     DDKQYMIPRNYFDCPRFSIAARKDLVSKYSDGGFETLEEYGDFLKAVKENENRILPGF
                     IYSRQFFDAYIKGNGYYMNNASWFYSKFDSKQPALYPITDLNEFIDAYNLLKNWKDNG
                     YVIANNSIYNYTDYLPSGGLASFMLSGRSLEDNIKLAVPGKFEYGIYPLYMNSIHELP
                     VSSRGLVVSKHSKNPQRVLMFIEWLHSSQENYDLFIYGIQDKNYELKGDNIYIPADMK
                     LLYWDIMGSDFFKDYRFARSSLIGTNESRKILLDASLKNVITSDELTEEIFGKDHNEL
                     FDDLLKDKEWQEEINNMYKNLNGIFDKYFDNMKTFCKYIDSGLFIMTPQKLQENQKEA
                     GVDKIIEYYEKVFERYRR"
     gene            4146..4910
                     /locus_tag="PK733_14685"
                     /pseudo
     CDS             4146..4910
                     /locus_tag="PK733_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            4912..5715
                     /locus_tag="PK733_14690"
     CDS             4912..5715
                     /locus_tag="PK733_14690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ11822.1"
                     /translation="MVDEKMKSTKLRTLISSLIFSIILMVFPVVSGAIVVINSIETLR
                     SYLMQGVFMMLSIAVPVIFMGITKTKPSQIGFTGMERGSVKIVLYFIPVIAAKIGFLL
                     YEINHDIHTVMSLAFFTIAIGLSEELYFRGIILRKLKACFTIKQTVIISSVLFAAVHA
                     SQAFSGTVLIMVALTIINAFIFGIVASEIVMLTKSIFPVIIWHTLYDFINWISLVSGT
                     TEVFLIIIQSICMVIYASYLWAKLPVPFKKSICQQYTTNKTIEHNKWYY"
     gene            6107..7054
                     /locus_tag="PK733_14695"
     CDS             6107..7054
                     /locus_tag="PK733_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11823.1"
                     /translation="MVEKMQNPISKTSLCRASFYKTFLDNYQLYLLMLPGIIVTVIFS
                     YIPMYGVQIAFKNFRTSRGIWRSEWIGFDHFARFVRYTGFWRMLRNTVSITLYNLATF
                     PVPVIIALLINEINSKKFKKTIQMISYAPHFLSTVVLCGMVLLFLNRSNGIINNIIAL
                     FGAERIDFMTRSEFFSSIYVWSGVWQNAGWGTVIYLAALSNVSPELIESVKIDGGNRL
                     HIIWHINIPAILPTLVILFIMSTGNVLSLGHEKILLLQNDLNLERSRVISTYVYEIGL
                     VGGQMSYSTAINLFNNVVNLSILTMVNFFAQKLSEVSLW"
     gene            complement(7119..7301)
                     /locus_tag="PK733_14700"
     CDS             complement(7119..7301)
                     /locus_tag="PK733_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11824.1"
                     /translation="MQEKIAIYGRIITEKDIETAIFIVDNFGSLSRTELAKTVCECLD
                     TVKYRGYSITNKKTSI"
     gene            7540..8517
                     /locus_tag="PK733_14705"
     CDS             7540..8517
                     /locus_tag="PK733_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11825.1"
                     /translation="MMLTIERYLKENIDDHVTITQWLNKNKLPIFLRDFYNFFEMIIL
                     NVPCILVEIRDETPTIDVIKKHIKRIKELANKEIVLYYKEITRYRRKSLIENRIPFII
                     EDGQIFLPFLGLDLKKTTQYVDEKTTNFSTSTQLAYLYFLYNKDVVVNTTEFARKMNF
                     TVMTASRALNDLYDAKLIRCKIGGKTGRSKEYQRIPDPEYFEIGQAYMKSPIKKVVYV
                     EKKPIGALIAGLDALAELSMINPPAHPIRAISHRQLNKQDLKIISNKDMIKDQRLVEL
                     EIWNYDPKLFTDKKYVDLMSLYSSLKEKKDERVEQALEEILRGEIWYTG"
     gene            8502..>8834
                     /locus_tag="PK733_14710"
     CDS             8502..>8834
                     /locus_tag="PK733_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11826.1"
                     /translation="MVHGLEKFREYFGEHGNKYVFIGGTACDILMEELGTPFRATKDL
                     GYLIIIFSNVSSLIFSFSSDQRYLKILERVTYSLCVITFDLAFEEVCLPKVVKKMKSI
                     CYLDCIRYY"
BASE COUNT         3091 a         1270 c         1620 g         2853 t
ORIGIN      
        1 taattttact aaaaagtgtg ttgagagaat aaagaaattc tcgggaataa taacagagca
       61 atctaaacgc cttgggcagt ggatggattg ggacaactcc tattataccc atactgatga
      121 aaatatcagc ggtatatgga actttcttaa aaagtgtcat accaatgatt gaactgaata
      181 ttggaattgc tttattgttt ttaatttcag gatttttagg aaaacttatg atagaacgaa
      241 gaaggtagta ttatataaag attgctaatg cttacatgga tacaccccgc ccgtattata
      301 ttgcaattta tatattgcaa taaaaattct aaaaactctt gacaagaatc gattacaaat
      361 gtaatatttt attaaaagat tacgtttgta atttattaac caatttacta aaccaaggag
      421 cggcatttat ggataaatct aagtcttcaa ggatatctga cgctgagtgg caggttatga
      481 atgtgatttg ggacacagat agaacaacct cccaggccat tgtcaagcag cttaaggcaa
      541 aaacaaattg gagtgaaatg actattaaga cacttatagg cagattggta aaaaagaagg
      601 ttgcaggata tgaagtagat aagaatgata aaagaatcta ttattattat ccgattatta
      661 aacgcgacca gtgtatcaga gatgaaacaa acatttttct aaaaaaattt tataatggtg
      721 cattgagttc catgttatca agctttgtga aaaaaaatga gcttacccgg gaagaaatgg
      781 atgagcttat aaaaattctg gaatccggta aaaaaaagga ggaatagccg ccatgtatag
      841 tgagtttttt aattggttaa tacaatcatc tttgaaggca tccttgatta ttctgattgt
      901 attattctta aggaaatttt ttgaagataa aattaatgca agatggtata tgatactctg
      961 gacgattgta actatcaggc ttattttgcc ctgggcgcct caaactaata ccagcatgtt
     1021 taatgttctg gatctttcag taaatgtaat acaaagcgca attattaccg gtgaaaatat
     1081 agaaaatgaa attgaaaagt caaaagcttc catcaacaag tcagaacaca ataaatcaga
     1141 atattttgag aatcacagcg agcaagatgt ggatacatat tactttgata tatctcctga
     1201 tataaactct gatacgcagc cttatgaagc atcagctata aacaaaacat tcgattcccg
     1261 attgaatgtt ttgggacatg atataagcca taatccacaa acacctccat tactatattt
     1321 gattttaata ttttggattg taggattact agccttctat tcatatatag ttttgataaa
     1381 tattaagttc tacaggaaaa tcaaagataa aaagcccatt actgacgaaa gaatctatta
     1441 tataaccgaa caatgcagaa agcagctttg cattaaaatc ccgattacat tatttgaaac
     1501 cggtgcagtt gaaggaccgt ccatatatgg aatatttaat cccataatat taattcccaa
     1561 aaacatgtta agcatccttt cggattcaga aatcagatgc atactaattc atgaatttat
     1621 gcattatagg agaaaggata atatcttcaa tttatttacc atggtaataa gcggattaca
     1681 ttggtttaat ccgttagtgc atattgcttt tgccatgatg aggcatgata gtgagctttt
     1741 atgcgattcc cgggtattaa aacatatatc aggcgaagaa tatatctatg gaaagacatt
     1801 aataaagctg gcttgcaatt tcgcaggcaa tagcttgatt atatcagaag gtatgggcgg
     1861 taatggtaaa ggattaaaag aaagaatcaa aagaatagct ggaactgccc gttataaggc
     1921 cggctggagt ataattgcta ttttgatggt aattatagta ggctgtacag gccttagtaa
     1981 tgaaaaggcc ccttatgaac ccgatatatt ctataattta gaacccgtta atctcaattt
     2041 ttacacgcag ggttataacg gcagtaccgc tgaaataggc gatatgctta aggaaattga
     2101 acaaaagctt accggctccc tgagaataac acctgttttt cattggatac cttatgaaga
     2161 atatgacgat aagcttaaaa cactaataac ctcaggtgaa gccgtagatg cctttacaag
     2221 ctatacgccg cggacatatg tacaagaaga tgttgccctg gatatatcag ccctgtttcc
     2281 acaatatgca ccggagtatt atgagcagct taaatccaat gaggccggtg aaatgcttct
     2341 cgaatcagta accgttgatg acaagcaata tatgatacca cggaattatt ttgattgtcc
     2401 gagatttagc atagcggcaa gaaaggatct agtttcaaaa tattcggatg gcggctttga
     2461 aacactggag gaatatggtg actttttaaa agcggtaaaa gaaaatgaaa acagaatctt
     2521 acctggtttt atatattcca gacagttttt tgacgcatat attaagggga atggttatta
     2581 tatgaataac gcctcctggt tctattcaaa gtttgacagc aagcagcccg cactttatcc
     2641 tataactgat ttaaatgagt ttattgatgc atataacctt ctcaaaaact ggaaagacaa
     2701 tggatatgtt atcgcaaaca attctattta taattatact gattatttgc cttccggagg
     2761 actagcatcc tttatgctga gcggaagaag ccttgaagat aatataaagc ttgcagtccc
     2821 tgggaaattt gaatacggta tttatccgct ttatatgaac tcaatccatg aacttccggt
     2881 atcgtcacgg gggctggttg tcagcaaaca cagcaaaaat cctcaaagag tgttgatgtt
     2941 tatagaatgg ctgcacagct cacaggaaaa ctatgatctg tttatatatg gaattcagga
     3001 taagaattat gagctgaaag gcgataatat ttatattccc gcagatatga aattgttgta
     3061 ttgggacata atgggatccg atttttttaa agattataga tttgcaagga gctcacttat
     3121 aggaacaaat gaatctcgta aaatactgct tgatgcaagc ttaaagaatg taattacatc
     3181 ggacgaattg acggaggaaa tttttggcaa agatcataat gagctttttg atgatctttt
     3241 aaaagacaaa gaatggcagg aagagattaa taatatgtat aaaaatttga atggcatatt
     3301 tgataaatat tttgataata tgaaaacctt ttgcaaatat atagattcgg gcctctttat
     3361 tatgactccc cagaaacttc aggaaaacca gaaagaagca ggtgtagata aaattattga
     3421 atactatgaa aaagtatttg aaagatatag gagatgagtt tcttttagca tctgatattt
     3481 gcaaatataa tatgatttta atctaaaggc tcattcaatg ccagcatgtt gaaataggta
     3541 gtaaacaata atattaagta ccgaaagaag atataaaata tgatatactt tataagtacc
     3601 aatttgtatt tgtggtaaat tttgaggctt acaggctgct catatagtat taatttaata
     3661 gtattttacc tcaatttaaa ttgtttgctt atattttacc aactaaggaa ataggatata
     3721 attttttata tgaaagattt tataaatcga agttcaaaca tagataattt ataaacttat
     3781 ttaaaatcgg tgttgcctta aggtgtataa agtatgaaag tttatttaaa gtttagtgga
     3841 taaatgaaaa tattaaagat attacgctga tgcttctaat aattaaaaca tacagataag
     3901 agaatattgt cgaatggtgg agagtgattt ataaagggat ttaagactta atgtagaata
     3961 atatatatat aggttaatct tgtaagctat tccaatcaag attaattagt aaagtttaag
     4021 gagaaagcaa atgagtacta aaacactaaa cacactggaa caattgattg aaaattataa
     4081 tatacaagga acattaacag aataaagaag ataaaaatta cacctattgt aatggtgggt
     4141 attggcgcat atgtcgttat gtagacaact cagttacata cgaaacagcg gaagatgcca
     4201 gggttctgtg tagtgcaggc tatgcttttg gacgtttcca atgcttgtta tcagactttc
     4261 caatagacaa gcttcatgaa actatactgg attttcataa tactaaaaaa agattaaatg
     4321 atttctttgg catggtagct ttagatcctg ttggcagagt aaaggatgta gaagaagaaa
     4381 taagattttt tgaaaacaac tgttcaaatg catcaaaact tatagatatg caggaaagcg
     4441 gccaacttcc tttacgtgta actcataacg atacaaaata caacaatatt ttaatggata
     4501 agtatacctg taagcctctt tgtgttatag accttgatac tgtaatgccg ggcctcagca
     4561 catatgactt cggtgatgct atccgctttg ctgctaacac tgcaatggaa gatgaaacag
     4621 atttatcaaa agctgagtta gatatgaaaa aatatgaaga atttacaaaa ggattcatga
     4681 cagcatcaaa agaatttcta acaaaagaag aactagatac catggcacta ggagcaataa
     4741 ttataaccat agaacttgca tcacgcttcc tggcagatca cataaacggt gacaaatatt
     4801 tccgcataca tagagaaaat cataatttgg atagggcaag atgccagatc aaactggctc
     4861 aggatatgat aaacaaatat gatgaaatgt gtaatataat gggagaataa aatggtagat
     4921 gaaaaaatga aaagcactaa attaaggacg cttataagtt ccctaatatt ttcgattatt
     4981 ttgatggttt tcccggtggt gtccggtgca atcgtagtta ttaatagtat agagacatta
     5041 cgaagttatt taatgcaagg tgtttttatg atgttatcca tagcggtgcc tgtaatcttt
     5101 atggggatta caaagactaa accgtctcaa atcggtttta ccggaatgga aagaggcagt
     5161 gtaaaaatag tattatattt tataccggta attgcagcaa aaattggctt tctcttatat
     5221 gaaattaatc atgatataca tactgttatg tcacttgcat ttttcacaat agcaatagga
     5281 ttgtcagaag aactttattt taggggaatt attctgagga agctaaaggc ttgttttaca
     5341 ataaagcaaa cagtcatcat atcttctgta ctctttgcag ccgtgcatgc ttctcaagca
     5401 ttttcaggca cagtgcttat catggttgcg cttaccataa ttaatgcttt tatatttggt
     5461 attgtcgctt cagaaattgt aatgttgaca aagagcattt ttcctgtaat tatatggcat
     5521 actttatatg attttataaa ttggatttcg ttggttagtg gaacaacaga ggtgttcttg
     5581 ataataatcc aatctatatg catggttata tatgcaagtt atttatgggc caaattacct
     5641 gtccccttca aaaagtccat ctgccaacaa tatactacaa ataaaaccat agaacacaat
     5701 aaatggtatt actgaagtat gaaataaaag gtaatatacc attaagtaca ggcgttgatt
     5761 aagctttgta ttagaaattt ctgttggagg gtattttagg taatggtata ggtgttttgc
     5821 aattgatacc ctgatgagtg cgatttgtat taataatagt tgtgaatgta ttctttaagc
     5881 agcctgtaat attgaataaa gcgcctttat agttatcggt gattattgct attggatgcg
     5941 acattaatgc tattcaattg tatcgaaata attatcggtg attattgcta ttacataaga
     6001 tatttatgct atttacaacc catagtcatc aaggtaagat taaaaacaag acaagattaa
     6061 gccgattaga tattttacat tactattgat taatgttaaa ggaaggttgg ttgaaaaaat
     6121 gcaaaaccct ataagtaaga catcgttatg cagagcatcg ttttacaaaa cttttttgga
     6181 caattatcag ttatatttgc taatgctccc cggaatcatt gttacagtta tattttcata
     6241 tattccaatg tacggagtac agatagcatt taaaaatttt cgtaccagtc gcggtatatg
     6301 gaggagtgaa tggataggat ttgatcattt tgcaaggttt gtacggtata ccggtttctg
     6361 gagaatgctt agaaataccg tctcaataac tttatataac ttagccactt ttcctgttcc
     6421 ggtcataatc gcccttttaa ttaatgaaat aaattctaaa aaatttaaaa aaaccattca
     6481 aatgatatca tatgctccgc attttctttc tactgttgta ctttgcggta tggttttatt
     6541 attccttaat agatcaaacg gtataataaa taatattatt gctttatttg gcgctgagag
     6601 aatagatttc atgacaagat cagaattctt tagcagtatt tatgtttggt ccggtgtatg
     6661 gcaaaatgca gggtggggta ccgtaattta tcttgcagcc ttatcaaatg tatcaccaga
     6721 gctgattgaa tcagtgaaaa ttgacggtgg aaataggctc catataatat ggcatatcaa
     6781 tatcccagct attctgccta ctttggttat attatttata atgtctacgg gcaatgtgct
     6841 ttcactagga catgaaaaaa tcttgttact acaaaacgat ttgaatctcg aaagatcccg
     6901 tgttatttct acatatgtat atgaaatagg tttagtaggg ggacagatga gctattctac
     6961 agctataaat ctatttaaca atgtagtaaa cctatctatt ctaactatgg tgaacttttt
     7021 tgcccaaaaa ctatcggagg taagtttatg gtgacattca acaaacgtac ctcaaagaaa
     7081 tttgatattg atattatctt taatgtattt aataccattt atatgcttgt tttcttattt
     7141 gtaatgctgt accctctgta ctttacagta tccagacatt cacatacagt ttttgctagt
     7201 tcggtcctgc ttaagctgcc aaaattatcg acgataaata ttgcagtttc aatgtctttt
     7261 tccgttatga ttcgcccgta aattgcaatt ttttcctgca tatacattct cctttatatt
     7321 tgttcccaat ttattttaac aaacactcga aagaaagttc agcactattt tgagaaaaaa
     7381 ttttttctgt gttccttgta accctgttaa attaacaatg ttcaattttg tgcgtcagca
     7441 ctgaataaat ttaaaaccta atataacatt tttaacttgc aatataacat aagaagtgtt
     7501 atatttaaag caaaaaatgt tatattagga ggtgactaaa tgatgctaac tatagaacgg
     7561 tacttaaaag aaaatattga tgatcatgtt actataacac agtggttaaa taaaaataaa
     7621 ctacctattt ttttgagaga tttttacaat ttttttgaga tgataattct taatgtccca
     7681 tgtatattgg ttgaaattcg agatgaaaca ccgactattg atgttataaa aaaacatatt
     7741 aaacgaatta aagaattggc aaataaggaa attgtacttt actacaagga gattacccgc
     7801 tatagaagaa agagtttgat tgaaaatcgt attccattta ttattgaaga tgggcagatt
     7861 ttcttaccct ttttaggtct tgatttaaaa aagacgactc aatatgttga tgagaagaca
     7921 acaaactttt ctacatctac acaactagca tacctttatt ttctgtataa caaggatgtt
     7981 gtggtcaata ctactgagtt tgctagaaaa atgaatttta cagtaatgac tgcttctcgt
     8041 gcattgaatg atttgtatga tgctaaacta atcagatgta aaattggtgg taaaacagga
     8101 cggagcaagg agtatcaacg gattccagat ccagagtatt ttgaaatagg ccaagcatat
     8161 atgaaatctc ctattaagaa ggttgtttat gtagaaaaaa aacctatagg tgctttgatt
     8221 gctgggcttg atgcattggc ggagctatca atgataaatc ctcctgcaca tccaataaga
     8281 gcaataagcc atcgtcagtt aaacaaacaa gatttaaaga ttattagtaa caaggatatg
     8341 attaaggacc agcgactagt agaattggag atatggaatt atgatcctaa actatttaca
     8401 gataaaaaat atgtggactt aatgtctttg tatagttctt taaaagaaaa aaaagatgag
     8461 agagtagagc aggctttaga ggaaattttg cgaggtgaga tatggtacac gggttagaaa
     8521 aattcaggga gtattttggg gagcatggga ataaatatgt ttttattggt ggaactgcct
     8581 gtgatattct tatggaagaa ttgggaacac cttttagagc gacaaaagat ttaggatatt
     8641 taattataat tttttcaaat gtttcatcgc ttattttttc tttttcatca gaccaaagat
     8701 atctaaaaat attagaacga gtcacgtata gtttatgtgt cattactttt gatttagctt
     8761 ttgaggaagt atgcttacct aaagttgtta aaaagatgaa atctatctgc tacctggatt
     8821 gcattcggta ttat
//
LOCUS       DAOSDD010000193         8756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2982_length_8756_cov_3.653258, whole genome shotgun sequence.
ACCESSION   DAOSDD010000193 DAOSDD010000000
VERSION     DAOSDD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2982_length_8756_cov_3.653258"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PK733_14715"
     CDS             <1..833
                     /locus_tag="PK733_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11827.1"
                     /translation="FVNQSIRDIPEKQRNELFESYNFMKEESHKFFEMAILLEGGNII
                     QIGQEYYTITKNQICIIDKNIKHRLGWDKSMPDCSSMLWIGITGETVRTSFTTYFSSG
                     HRKISGMDIYGTGEFFVNEICNELRTKNDGYKEVVSAYLSAFLTLLARKLSSPSISIG
                     KFERVQIVGKAKEYIKSHLDSHLTLQEISNKVYVSPNYLSTLFKQVTGQTITQYIQDL
                     KIRRAAKLLKMSDLSLLEVAESLGFYDQFHFSKVFKSFTGISPAAYRNSMLYTPKTSI
                     "
     gene            1128..2615
                     /locus_tag="PK733_14720"
     CDS             1128..2615
                     /locus_tag="PK733_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOJ11828.1"
                     /translation="MRINNNIMAMNTYRQLGLGSAAGARSMEKLSSGFRINRAGDDAA
                     GLAISEKMRGQIRGLTQASRNAQDGISLIQTAEGALNETHAILQRMRELAVQSATDTN
                     VGVDRGEIQKEVDQLAKEITRISNTTEFNTQNLLAGGFDGTFHIGANKGQNVNLSIGQ
                     MDAKALGVAGSNIIQTGGLAGNGSGIASVANLSAELEAGTDYAIDVTKTAADVGAAIA
                     KGTGDGTAAETGSIDYTGFTGTSNTTYTIEVVTVSGDADANQITSIRVSNDGGKSWSG
                     SIDISGGTGTFDGLVYTREVAGAGSRYAAGDQFTFTANASYADVQLQKGGAGIGDAVR
                     VYYGATSANIGNVQDGKTLTANFKYTDLATATTSDFTIAEQESESASISNGVITKEAL
                     TRAGTNVSTQKSADAAIIKINSAIETVSAERSMLGAYQNRLEHTIKNLDTSAENLQAS
                     ESRIRDVDMAKEMMEFTKNNILQQAATAMLAQANAAPQAVLQLLR"
     gene            3032..4753
                     /locus_tag="PK733_14725"
     CDS             3032..4753
                     /locus_tag="PK733_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ11829.1"
                     /translation="MIRRFVSYYRPHIKLFLADLTCAFSMASIDLVFPVVTRYLLQQV
                     IPNNNIKLMLGIIFALTGMYLVRMLLNYFVNYYGHIVGIRMEYDMRRDLFSHLQILSF
                     KFYDTVRTGKLMSRMMNDLNEITELAHHGPEDIFLSVIMLFGSVVILSTIEWRLTLVV
                     FAIIVFMAWFAITKRTKMSRAFRDVRKKVANVNAQVESSISGIRVSQAFANEEFEKQK
                     FDEGNTQFKGSKDEAYRYMAQYSAGIDFITNVLDISVIGFGGYLVYIKSINIVDLMTF
                     LLYINLAVQPVRRLAQFVQQFESGMAGFERFCEIMDERPHIVEKVNAKELKDVKGEIS
                     FEDVTFAYNEGEDILRKVNLIIPAGKVVALVGPSGGGKTTLCNLIPRFYDIEAGSVKI
                     DGNDVRDLTLKSLRNNIGIVQQDVFLFAGTIKDNIFYGKVDATDEEVMEAAKRANIHE
                     FIMSLPNGYDTYVGERGIRLSGGQKQRISISRIFLRNPPILILDEATSSLDNETEMII
                     QESLEELSKGRTTVIIAHRLSTIRNADEIVVLDKDGIQERGTHNDLVKLDGIYSRLYN
                     TQFEENV"
     gene            5017..7188
                     /locus_tag="PK733_14730"
     CDS             5017..7188
                     /locus_tag="PK733_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOJ11830.1"
                     /translation="MSDIISRLIKEFKLKPFQVENTVKLIDEGSTIPFIARYRKEMTG
                     ELNDQVLRELYERLIYLRNLESRKEDVKRLIDEAGKLTGDIVVALKRAETLQEVEDIY
                     LPYKPKRRTRAAIAREKGLEPLAGILILQVKLKGTIKDIAGPFINPEKGVESVEDALN
                     GAMDIIAEEVSDNAEHRKTVREVFLRNGVIITKAKKEEDSVYRMYYDFKEPVDKIASH
                     RILAINRGEKEGYLQVKIDVPDEEIITRLKRKVVKNPHSETSQYVESAVEDAYKRLIF
                     PSMENEIRNAITETAEEQAMVVFRENLKNLLMQPPIKNKIVLGLDPAYRTGCKLAVVD
                     GTGKVLDTAVIYPTPPQNKVEEARKVVKYLIDKHKVDIVSIGNGTASRESELFIADLL
                     KEIDRKVHYIIVSEAGASVYSVSKAGAEEFPDFDVSQRSAVSIARRLQDPMAELVKID
                     PKSIGVGQYQHDMNQKRLDESLTGVVEDSVNKVGVDLNTASPSLLSYISSVNTTLARN
                     IVGYRETNGKFKNREELKKVKRLGQKTFEQCAGFLRISEGENILDNTAVHPESYDAAL
                     KLLKAMGYSLESVKNRKLGDLKKKVEKYSEGFGIEKLAQIIGAGVPTLMDIIDELSKP
                     GRDPRDELPKPVFHTDVMKIEDLKAGMMLTGTVRNVANFGAFVDIGVHQDGLVHISEM
                     SDRFVRNPMDIVSVGDIVKVKVLNVDVEKKRISLSMKGIKL"
     gene            complement(7399..8115)
                     /locus_tag="PK733_14735"
     CDS             complement(7399..8115)
                     /locus_tag="PK733_14735"
                     /inference="COORDINATES: protein motif:HMM:NF023053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HOJ11831.1"
                     /translation="MIPLEKINEKIEKMIAPIRNNISLVKKIAFITFGILIVLSLILG
                     IYNRSVLSGKISKLEKELDTLSSDIDQFKNDNENLTAELQNFNELKVKILTDLAINKL
                     RLDQYSKAVFNAADSSKGFARLDSSTGMFFLILDKTEQFSDGYKLYFRLGNPQNCVYN
                     GCKVKIRWGQKYDRENEDVSYEDWEKSLKSSELSIKDMLIPGQWCSFDVAISPAKSED
                     VQYVELKIQAEQISMQKSKD"
     gene            8511..>8756
                     /locus_tag="PK733_14740"
     CDS             8511..>8756
                     /locus_tag="PK733_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11832.1"
                     /translation="MSPFEIIMLACFGAAWPASIYKTYKSRKNTGKSLTFLIIVFFGY
                     VSGVIHKIFFNYDAVIFLYILNSIMVFMDMMLYFRNKK"
BASE COUNT         3044 a         1330 c         1774 g         2608 t
ORIGIN      
        1 tttttgttaa ccaaagcatt agggatatac ctgaaaaaca gcgaaatgag ctgtttgaaa
       61 gctacaactt tatgaaagag gaaagccata aattttttga aatggctatt cttttagagg
      121 gtggcaatat aattcaaata ggtcaggagt attatacaat taccaaaaat cagatctgta
      181 tcatagataa aaatataaag caccggttgg gctgggacaa gtccatgcct gattgctcgt
      241 ccatgctgtg gataggcata accggagaaa cagtacgcac cagcttcacc acatattttt
      301 cctcgggaca tagaaaaatt tccggcatgg atatatacgg aaccggtgaa ttttttgtaa
      361 atgaaatctg taatgagctc cgaacaaaaa atgatggtta taaagaggtt gtttctgctt
      421 atctttctgc ttttcttact ctgcttgcca gaaagttaag ttcgccgagt ataagcatcg
      481 gtaaatttga gagagttcag atagtaggta aagctaagga gtacatcaaa agccatctgg
      541 attcccatct tacgcttcag gaaatatcga acaaggtcta tgtaagccca aattatttaa
      601 gcacgctttt caaacaggtc acagggcaaa ctattaccca atatatacag gatctcaaaa
      661 taagaagggc tgctaagctt ttaaaaatgt cagatttgtc cttattagag gtggctgaat
      721 cgctgggatt ttatgaccag ttccatttca gcaaggtctt taagtcgttt accggaatat
      781 ccccggcagc atacagaaat tcaatgcttt atacgccaaa gacgagcatc taattaaaac
      841 aatgactggt tatcacttta tcattttttt tcaccggtac aaagattaac acatcaaaaa
      901 taataaaacc aaaaaaatga aattatctgt aaagttttca aaggattaat cgatatatat
      961 agtagggtgg aataaggtgt ctattcatag cggccgtatg aaaagcacat tattcactgt
     1021 ttacttacat tatttaattt aacttcaaga gtcatgcaaa aagcaaatta aacaaaaaca
     1081 aagcacgggc aaggatgccc ggcggaaaaa caaggaggta ttattttatg agaatcaata
     1141 acaatataat ggcaatgaac acatacaggc agttgggatt aggaagtgca gcaggtgcca
     1201 gatcaatgga aaaactgtca tcaggcttta gaatcaacag ggcaggagat gacgcagccg
     1261 gtttggcaat tagtgaaaaa atgagaggac agatcagagg cttaactcaa gcttccagaa
     1321 atgcacaaga tggtatttct ttaattcaaa cagcagaagg tgcattaaat gaaactcatg
     1381 caatccttca gagaatgaga gaacttgcag ttcaatcagc aactgataca aatgtaggtg
     1441 tagaccgtgg agaaattcaa aaggaagtag accaattagc aaaagaaatt actcgtatat
     1501 ccaatactac agaatttaac actcagaatt tattagcagg tggctttgat ggtacattcc
     1561 acatcggtgc aaataaaggt caaaatgtta atttatctat agggcagatg gatgcaaaag
     1621 cactaggagt tgctggttct aatatcatac aaacaggcgg acttgctgga aatggaagtg
     1681 gaatagcttc agttgcaaat ttaagcgctg aattggaagc tggtacagac tatgcaattg
     1741 atgttacaaa aacagctgca gacgtaggtg cggcaattgc aaaaggtact ggagatggca
     1801 ctgctgctga gacaggttct atagactata ctggatttac aggaacttca aatacaactt
     1861 atactattga agtagtaaca gttagtggag atgcagacgc aaatcaaatt acttctatca
     1921 gagtatctaa tgatggcgga aaatcttgga gcggatctat tgatattagt ggtggcacag
     1981 gaacttttga tggattagta tatacaagag aagtagctgg ggcaggatct agatatgctg
     2041 caggagatca atttactttc acagctaatg cttcatacgc tgatgtacaa cttcaaaaag
     2101 gcggagcagg tataggtgat gctgttagag tttattacgg agcaacaagt gccaacattg
     2161 gtaatgtgca agatggaaaa acgttaactg cgaactttaa atatactgat ctagcaactg
     2221 ctacaacaag tgattttaca atagctgaac aagaatcaga atctgcctcc atatccaatg
     2281 gggtaataac taaagaagcg ttaaccagag caggtacaaa tgtatcaact caaaaatcag
     2341 cagatgctgc tattataaag attaactctg caatagaaac agtctcagca gagaggtcta
     2401 tgcttggtgc gtatcaaaac agattagagc acacaataaa gaatcttgat acatcagctg
     2461 aaaacctgca ggcatcagaa agcagaatca gagatgtaga catggcaaag gaaatgatgg
     2521 agttcacaaa gaacaacatc ttgcagcaag ctgcaacagc aatgctggct caagcaaatg
     2581 ctgcacctca agcagtactt cagttgttaa gatagctgaa tatccgggaa taacaattaa
     2641 ggggtcaggg gagttgctcc tctggccctt tttaattgct atacaaagaa aagtcaacta
     2701 tcggaaaata atattctagt tgaagctggt tgctccaaag ttaggtatga gtattgacat
     2761 cataataaag gcaacaggat tttctgcgtt gtgaattacg aatttttcct tgctctaaac
     2821 atagtaatcc gtaaattttg ttcaaattat tatatttaag aaattctatt ctaccatttc
     2881 tgccatatta tatagcagaa aatttaatac acttccattg aaaatgatat tatattgaca
     2941 aaattcggtt tttattgtaa tcatataata gagctattat agggatatta aagagaatta
     3001 aaggagatta cttttaatgg tgtccaaaaa aataataaga agatttgttt catattatag
     3061 gccgcatata aaattatttt tggcagattt aacctgtgca ttttcaatgg catcaataga
     3121 cttagtattt ccggtagtga ccagatattt actgcaacaa gtaattccaa ataataatat
     3181 taagttgatg cttggtatta tttttgcatt aactggcatg tatctggtta gaatgctgct
     3241 taattatttt gtaaattatt acgggcatat tgtaggaata agaatggaat atgacatgag
     3301 aagggatctt ttttcccatt tgcaaatact atctttcaag ttttatgata ctgtcagaac
     3361 aggaaaactt atgtccagaa tgatgaatga cctgaatgaa ataaccgaac ttgcccatca
     3421 cggacctgaa gacatatttc tttcagttat tatgcttttt ggctctgttg ttatcctttc
     3481 tactattgaa tggaggctta ctcttgttgt ttttgctata attgttttta tggcatggtt
     3541 tgccataact aaaaggacaa aaatgtcaag ggcctttagg gatgtaagga aaaaggttgc
     3601 aaatgtaaat gcccaagttg aaagtagcat ttcaggaata cgggtttccc aggcttttgc
     3661 aaatgaagag tttgagaagc agaagtttga tgaaggtaat actcagttta aaggttcaaa
     3721 agatgaagca tataggtata tggctcagta ttcagcggga atcgacttta ttactaatgt
     3781 actggatata agtgtaatcg ggtttggagg atatcttgtt tatataaaat caataaatat
     3841 tgtcgatttg atgactttcc tgctttatat taatcttgca gttcaacctg ttagaagatt
     3901 agcgcagttc gtgcagcagt ttgaatcagg aatggcagga tttgaacgtt tctgtgagat
     3961 tatggatgaa aggcctcata ttgttgaaaa ggtaaatgca aaagaattaa aggatgtcaa
     4021 aggagaaata agctttgaag atgttacctt tgcctataat gaaggtgagg atatacttcg
     4081 gaaagtcaac ttgataatcc cggctggaaa agtggttgca ctggtagggc cttcgggtgg
     4141 cggaaaaact acattatgta acctaatacc aagattttat gatatagaag caggttctgt
     4201 taaaatagac ggtaacgatg taagagattt gacgcttaaa tccttaagaa ataacatagg
     4261 aattgtacaa caggatgtat ttctttttgc aggaactata aaggataata tattttatgg
     4321 taaggttgat gcaaccgacg aagaagttat ggaagctgcg aagcgtgcca atatacacga
     4381 atttatcatg agcttaccaa atggttatga tacttatgta ggagaaagag gcataaggct
     4441 ttctggagga caaaaacaga gaatatccat atcaaggata ttcttgagga atccaccaat
     4501 attaatactt gatgaagcaa cttcatctct tgataatgaa acggaaatga taatacagga
     4561 atcgttagag gaattatcaa aaggaagaac caccgttatt atagctcata ggctatctac
     4621 aataagaaat gcggatgaga ttgttgtttt agataaggat ggaattcaag aaaggggtac
     4681 tcataacgat cttgtaaaat tggatgggat atattcaagg ttgtataata ctcaatttga
     4741 agagaacgtg taggaagtaa gagaggaagc tgatatgaaa ccggataatg ctatatataa
     4801 tgccttacat aatgccatat ataatgctat acataatgcc acacataatg ccatatataa
     4861 tgccatcatg tataattcta tatataatgc catatataat gctatacatt atataaaaaa
     4921 tgcgcccgca tgctttccaa aacttgtgtt tataaagtag caaggatata gaatatatgc
     4981 taaaataaag ctgaaaatat attgaggagg aatataatgt ctgacataat ttccagactt
     5041 ataaaggaat ttaaactaaa accttttcag gtagagaata ctgttaaact tatcgatgaa
     5101 ggttccacca taccttttat tgccagatat aggaaggaaa tgacaggcga gctaaatgac
     5161 caggttttaa gagaattata tgaaagatta atatatttaa ggaatttaga atcaaggaag
     5221 gaagatgtaa agagactgat agatgaggca ggtaagctta ccggcgatat agttgttgct
     5281 cttaaaaggg ccgaaactct tcaggaagta gaggatatat atctgcctta taaaccaaaa
     5341 cgtcgcacaa gggctgccat tgccagagaa aaaggacttg agccacttgc cggaatttta
     5401 attttacagg taaaacttaa aggaactata aaagatattg ccgggccttt tataaatcca
     5461 gaaaagggtg ttgaatctgt tgaagatgcg ttaaatggag caatggatat tattgcagaa
     5521 gaggtatctg ataatgctga acatagaaag actgtcaggg aggtgttttt aagaaatggc
     5581 gtaatcataa ctaaagctaa aaaagaagaa gactcagtat ataggatgta ctatgatttt
     5641 aaggaacctg tagataaaat tgccagccat aggatattgg ctattaacag gggagaaaag
     5701 gaaggatacc tgcaagtcaa aattgatgtg cctgacgaag aaataattac ccgtcttaag
     5761 agaaaagtag taaagaatcc gcattcagaa acttctcaat atgtagagag tgctgtagaa
     5821 gatgcgtata agcgtctaat atttccttca atggagaacg aaataagaaa tgccataact
     5881 gaaacagcag aagaacaagc aatggtagtt ttcagagaga atctaaaaaa tctgctgatg
     5941 cagccaccta taaaaaataa gatagtgtta ggattggatc ctgcatatag aacaggatgt
     6001 aaacttgctg tagttgatgg aacaggtaaa gtgcttgata ctgctgtgat atatcctact
     6061 cctcctcaga ataaagtgga agaggctaga aaagttgtta agtacctaat agacaaacat
     6121 aaggttgata tagtatcaat cggaaatgga accgcatcac gagaaagcga attatttatt
     6181 gcagatcttt taaaggaaat agacagaaaa gtgcattata ttatagtcag tgaagcaggg
     6241 gcatcagttt attctgtgtc aaaagccggc gctgaagaat tcccggattt cgatgtatcg
     6301 caaagaagtg ctgtatctat tgccagacgt ttacaagatc ccatggctga attggttaaa
     6361 atagatccta aatccatagg tgttggtcaa taccagcatg atatgaatca aaaaaggctg
     6421 gacgaatcct tgacaggagt agtagaagac agtgttaaca aggttggagt ggatttaaac
     6481 actgcttcac cttctctttt gtcttatata tctagcgtta atacaacact tgcaagaaat
     6541 attgtgggat atagagaaac taatggcaaa tttaaaaata gagaggaact taaaaaagta
     6601 aaaaggttgg gacaaaaaac tttcgagcaa tgcgcaggtt ttttacgtat ttctgaagga
     6661 gaaaatatct tggataatac tgctgtacac cctgaatcat atgatgcagc gttaaagctt
     6721 cttaaggcta tgggatatag cttagagtct gtaaaaaatc gaaaattagg tgatttaaag
     6781 aagaaagttg aaaaatacag cgaaggattt ggtattgaaa aattagctca aattattggt
     6841 gcaggggtac caactctcat ggatattata gatgagcttt caaagccggg acgagatcca
     6901 agagatgaac taccaaaacc tgtttttcat accgatgtaa tgaaaattga agatcttaaa
     6961 gcaggcatga tgttaaccgg tacagtgagg aatgttgcta attttggagc ttttgtcgat
     7021 atcggagttc accaggacgg attggtgcat atatcagaga tgagcgatcg ctttgtaaga
     7081 aatcccatgg atatagtatc ggtaggagat atagtaaagg taaaggtgct taatgtagat
     7141 gttgaaaaga aaaggatcag cttgagcatg aaaggcatta aactgtagag gctgtaggga
     7201 tttgtcttgc agcctttgta gaagaaatag ctggttacag gtctaaaaga ctaagtaaga
     7261 agccatttcc agtttgggta gggtaggata ggatagagta ggatagagta gtattttcga
     7321 aagctttaat tcattgtgct ttcaaaaaaa aattattgct acaaatattg caaccggaaa
     7381 tgattcacta cttagtggtt aatctttgct tttttgcatg cttatttgct ctgcctgaat
     7441 ctttagttca acatattgaa catcttcaga tttagcagga gaaattgcaa catcaaagct
     7501 gcaccactgt cccgggataa gcatatcttt tatagatagt tcactgcttt ttagggattt
     7561 ttcccaatct tcataactta catcctcatt ttccctgtca tacttttgcc cccatcttat
     7621 tttaacctta cagccattgt aaacacaatt ttgagggttc cccaacctga agtacaattt
     7681 atatccatct gagaattgct ctgttttatc aagtatcaga aaaaacatac cagtgcttga
     7741 gtctaatctt gcgaatcctt tgcttgaatc tgcagcatta aaaacagctt tactgtattg
     7801 atccagtctt aatttattaa ttgcaagatc tgtaagtatt ttaaccttaa gctcattaaa
     7861 attttgcagt tctgcagtca gattttcatt atcattttta aattggtcaa tatcagaaga
     7921 taaagtatct aattcttttt ccagttttga tattttccct gatagcactg atctattata
     7981 gatgccaagt attaatgaca acacaattag tataccaaag gttataaaag ctattttctt
     8041 aactaatgaa atattattac gtattggtgc tatcattttt tctatctttt catttatctt
     8101 ttccaagggt atcatcgtta ccaacacctc acattaaatt tagccatttc taaatgaatg
     8161 gaacataata ctattgtaca attaatttat ggcaatatgc aaatattttt ttattccctt
     8221 tttatttcca taaggtgcaa cttcaaatgc tgtacattta gttcttcaac ttgctgtgta
     8281 cctatagctg tgtacctata gctgtgcact tatagctgtg tacttatagc tgtgtaccta
     8341 tagatgtgta cctatagatg tgtacctata attagaataa gcagttgaaa tattttatga
     8401 aacatagtat gttattgttg aggcatttgt gtccaacgta ataaccttgc aaagacactg
     8461 gcaataatag ctgcgttact aaaactatta ctttggaggt actatattat atgagtccgt
     8521 ttgaaataat tatgctagct tgttttggtg ctgcctggcc ggcatcaatt tacaaaactt
     8581 acaaatcaag aaagaataca ggaaaaagcc tgacattttt aattatagtt ttttttggtt
     8641 atgtttcagg tgttatacat aaaatattct tcaattatga tgctgttatt tttctatata
     8701 tattaaacag cattatggtt tttatggaca tgatgctata ctttagaaat aaaaag
//
LOCUS       DAOSDD010000194         8735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_2995_length_8735_cov_4.802304, whole genome shotgun sequence.
ACCESSION   DAOSDD010000194 DAOSDD010000000
VERSION     DAOSDD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2995_length_8735_cov_4.802304"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1781
                     /gene="dnaA"
                     /locus_tag="PK733_14745"
     CDS             447..1781
                     /gene="dnaA"
                     /locus_tag="PK733_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOJ11833.1"
                     /translation="MNNQLSSIWQRTLELLKDEVTEISYNTWIKTIMPVSISANTIEL
                     GAPADFNRGILESRYSLLIQQAIKKATNREYKIVFIVPSQEGSMPSRGSVKKANEEDS
                     LISVLNPKYTFDTFVIGSGNQLAHAASLAVAESPAKSYNPLFIYGGVGLGKTHLMHAI
                     GHYVIQQNSSLKVLYVSSEKFTNELINAIKDDKNEAFRQKYRNIDILLIDDIQFIAGK
                     ERTQEEFFHTFNALYEANKQIIISSDKPPREIVTLEERLKSRFEWGLQADIQPPDFET
                     RIAILKKKAQVENIDIPDEVYSFIADNIASNIRELEGALIRVIAYSSLVKSEINLDVA
                     KEALKDILLANKTRVINSDVIIDVVSRHFNLKPDDFKSKKRSRDVAFPRQIAMYLCRE
                     LTDMSLPQIGSYFGGRDHTTVMHAFSKISDSIIDNYEIRRTVEELRRNISGG"
     gene            2032..3129
                     /gene="dnaN"
                     /locus_tag="PK733_14750"
     CDS             2032..3129
                     /gene="dnaN"
                     /locus_tag="PK733_14750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ11834.1"
                     /translation="MKIFCNKDDLVNAINTVQKAVPAKTTLPILQGIMVKAEDNLKLT
                     ANDLEMAIECSIKAEIIEKGSIVIDSKLFGEIIRRMPNSEVHIEVDNEENIKIDCKNS
                     KFEIKGTKSEGFPGIQEIQEDISLKASQSVIRDMIRQTIFAVGEDENRLILTGSLIEC
                     KNGLLTFVSIDGFRLALRKCIIEDQTAKISVIVPGKTLNEISKILNPVEDDVNIYASK
                     SMIMFDMGDTKVVSRLLEGEYFKYENVIPEEFETKIRINKKELQDSIERASLLISSDG
                     SRYPVTLLLKSDKLIITTNTQIGNAREEINIDIWGDELEIKFNPRYFIDSLKAIEEEN
                     VDVMFSSDVGPCILKSVDKDTFIYMILPLRK"
     gene            3157..4293
                     /gene="recF"
                     /locus_tag="PK733_14755"
     CDS             3157..4293
                     /gene="recF"
                     /locus_tag="PK733_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOJ11835.1"
                     /translation="MYIKDLKLNNFRNYKELYSEFNMDYNIICGKNAQGKTNILEAIF
                     LCSSGRSHRTSKDNELIKFLENSYYVNVDVNRKYTDTTIEIYYEKDSRKKIKINSVPS
                     KKNGELMGQLNTVIFSPEDMNIIKGSPSERRRYIDIAISQLKPSYFYNLQQYSKIIAQ
                     RNCLLKTLNINNGIKSKMYETLDIWNEKLVETGSRIMIERYNFLKELNEKIKEKHIKL
                     TGGREELEIRYAPSILIENGLDKKSIENSFYKKLVSSVEREIKNCATLYGPQRDDMEV
                     LVNEKNVKLFGSQGQQRTVVLSLKLSEVDIAYRLINEYPVLLLDDVFSELDIKRQQYL
                     IESIKGMQVFITTTEINFIKTLKENNNLSLYMVEEGVVKDTNII"
     gene            4390..4662
                     /locus_tag="PK733_14760"
     CDS             4390..4662
                     /locus_tag="PK733_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOJ11836.1"
                     /translation="MFLHIGGDVVIPIRNIIAIFDLEKTTISKDTKEFLKTAEEEGFI
                     ESISDDIPKSFVVTETDKKSKIYLSPISSTTLQKRSGFIKEMTFTK"
     gene            4709..6634
                     /gene="gyrB"
                     /locus_tag="PK733_14765"
     CDS             4709..6634
                     /gene="gyrB"
                     /locus_tag="PK733_14765"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOJ11837.1"
                     /translation="MTENNLNKIEYDENQIQVLEGLEAVRKRPGMYIGSTSSKGLHHL
                     VYEIVANSIDEALAGRCDKIKITVEKDNYITVEDNGSGIPVGPHPKLGIPTIEVVHTI
                     LHAGGKFGGGAYSVSGGLHGVGASVVNALSEYMIVEVSRNGYIYSQRYERGKAVTKLE
                     IIGNTENTGTKTTFKPDPFIFEEVVFDFEGMISRYREMAFLNKGIKIILIDNRPEEPV
                     EKTLHYEGGIISFVEYINKNKEVIHKPPIYIEGGKDSTVVEIALQYNDGYAENVYSYA
                     NDINTTEGGTHLTGFRSGITKVINDYARKYNILKESDKNLMGEDVREGLTAIISVKLL
                     EPQFEGQTKTKLGNSEVRGIVENVVSEKLEYYLEENPAEARAIIEKCTMAARAREAAR
                     KARELTRRKSALESTALPGKLADCSEKDPSLCEIYIVEGDSAGGSAKQGRDRKFQAIL
                     PLWGKMLNVEKARIDRVYGNDKLMPVITALGTGIGEEFDIEKLRYHKVIIMADADVDG
                     SHIRMLLLTFFFRYLKPLVEKEYIYIARPPLYKITKGKEHMYAYDDLDLEKIVREKEW
                     DKKECTIQRYKGLGEMSAEQLWDTTMNPETRSIIKVEVEDAVAADEIFSDLMGEKVEP
                     RKQYIHQHAKEVVNLDI"
     gene            7047..7826
                     /locus_tag="PK733_14770"
     CDS             7047..7826
                     /locus_tag="PK733_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ11838.1"
                     /translation="MAKIIAIANQKGGVGKTTTAINLSACLAYKGKKVILIDNDPQGN
                     STSGLGFEKRKLKKSTYDILINDEDIENVILKTCIDNLELCPSNIQLAGAEIELVTAF
                     SRETRMKNAINKIKDRNDFIIIDCPPSLGLLTINSLTAADTILVPIQCEYYALEGLSQ
                     LMDTVKLVQRHLNTDLDVEGVVLTMFDARTNLSIQVVEEVKKHFRNKVYRTVIPRNVR
                     LSEAPSYGLPIILYDSKSKGAECYMDLADEVIECSEGFNND"
     gene            7819..8685
                     /locus_tag="PK733_14775"
     CDS             7819..8685
                     /locus_tag="PK733_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ11839.1"
                     /translation="MTKKRLGRGLDALITIDENENDSIKEMKINDIEPNTAQPRKKFD
                     DESLNELAESIKKHGVIQPIIIKKQNDMYKIVAGERRWRAARIAGLDSIPVIVKDLSE
                     KEIVEVALIENLQREDLNPIEEAEAYEQLISEYEMTQEEISQIVGKSRSAIANTIRLL
                     GLCDEIKRNIIDGKITSGHARALLTIENKEVQKKLMEEIIKMNYNVRDTEKLVKKYEN
                     YKETETKNINNLKSEIRNQEIIELEEVLKNILGTKVKLNDEKKKGKIIIEYYSNDELD
                     RIVELIRKIEKQ"
BASE COUNT         3471 a         1014 c         1680 g         2570 t
ORIGIN      
        1 tgaagtgaat attatacatg gaaatcgttt cttgccatat ttttcttcaa atatacaaaa
       61 tgcacccttc attaaaaaac acgatgcctg tggataattg tattaatatt tattttttct
      121 gtggataact ttataaattt atattgaata tgattggaaa gtctgatata ttaattatac
      181 ttatcgtgat aaaactaatt aacatgtaat tagttttatt attagatatt aattgtttaa
      241 aatgtagtaa tttgatacat ttgcagtagt aattaaatgt atttaatgta ataaattatt
      301 cggcagtttt tttaataata catttaacaa tacaaaagaa aaggacatgc ctatgcatca
      361 atagcattat ttcattagtt aggcttaatg ttctttttgt ttgttaataa ttattagaga
      421 taaagtattt atatggagga aatggtatga ataatcaact ttcgagtatt tggcagagaa
      481 ctctcgaact gttgaaagac gaagttacag aaataagtta taatacttgg ataaaaacta
      541 ttatgcctgt atcaataagt gcaaatacta tagaactggg tgcccctgct gattttaata
      601 gaggcatact tgaatctagg tattcattat taatacaaca ggcaataaag aaagctacaa
      661 accgggaata taaaattgta tttatagttc cttcacagga aggcagcatg ccctcaagag
      721 gttcggttaa aaaagctaat gaagaggatt ctcttatttc tgtcttaaac ccgaaataca
      781 cttttgatac atttgttatt ggaagtggaa atcagcttgc acatgcagca tcacttgctg
      841 tagctgaatc accggccaag tcatataacc ctctttttat ctacggagga gtaggtttag
      901 ggaaaaccca tcttatgcat gcaattggcc attatgttat acagcagaat tcatctttaa
      961 aggttcttta tgtatcttct gaaaaattca cgaatgaact tattaacgct attaaagatg
     1021 ataaaaatga ggctttcagg caaaagtaca gaaatattga tatcctttta atagatgata
     1081 ttcagtttat agctggaaaa gaaaggactc aggaagaatt tttccacact tttaatgctt
     1141 tatatgaagc caacaagcaa attattatat caagtgataa acctccaagg gaaattgtaa
     1201 ctctggaaga aagacttaaa tccagatttg agtggggact tcaggcggat atccaacccc
     1261 ctgattttga gacacgaata gctatattga aaaagaaagc tcaggttgaa aatattgata
     1321 tacccgatga agtgtattct ttcattgcag ataatatagc atcaaatata cgtgaacttg
     1381 aaggagcgtt aatacgcgta attgcttatt catcacttgt aaaaagcgaa ataaatctgg
     1441 acgtagccaa agaggcatta aaagatattt tacttgcaaa taaaacaaga gttattaatt
     1501 cagatgtaat aattgacgtg gtttccaggc actttaactt aaagcctgat gacttcaagt
     1561 caaaaaaacg cagcagagat gtggcctttc caagacagat agcaatgtat ttatgccgag
     1621 aactgactga tatgtcgctt ccccaaatag gttcttattt tggaggaaga gaccatacta
     1681 cagtaatgca tgcatttagt aaaatatcag atagtattat tgataactac gaaataagac
     1741 gtacagtgga agagttaaga agaaatatat ccggtggttg agaaagagtg tttatttttt
     1801 ttactcaaaa tttagtccac aaaaaatgtt gatatgttga acttactctg tggacaaaga
     1861 agttattttt gttttacctt gaataatgtt aattaaataa taaaatatct acaagtttac
     1921 ccacagactc aaactttgat ttagcgtatg cttgactagg atattcacat taataacaaa
     1981 gcttactatt actatgacta ttttttatat actatatagg aggaaagagc gatgaaaata
     2041 ttctgcaata aagacgacct ggtaaatgca ataaatactg ttcaaaaagc tgtgcctgca
     2101 aaaacaacat taccaatttt gcagggaatt atggtaaaag ctgaagataa cctaaagctt
     2161 acggcaaatg atctggaaat ggcaattgaa tgcagtatta aagctgaaat tattgagaaa
     2221 ggctctatag ttattgattc aaagctattt ggtgaaataa taagacgaat gcctaattca
     2281 gaggtacata tagaagtaga taatgaagaa aacattaaga ttgactgtaa aaattcaaaa
     2341 tttgaaatta aaggtaccaa aagtgaagga tttcccggaa tacaggaaat acaggaagat
     2401 atttcattaa aagcaagtca gtctgttatt agagatatga taaggcaaac aatattcgct
     2461 gtaggagagg atgaaaaccg tctgatttta acaggatctt tgattgaatg taaaaatgga
     2521 ttacttacat ttgtatctat agatggtttc aggctggcat taagaaaatg tattattgaa
     2581 gatcaaactg ctaaaattag tgttattgtt ccgggaaaaa ccttaaatga aatatcaaaa
     2641 atattaaatc cagttgagga tgacgtaaac atctatgcaa gcaaaagcat gattatgttt
     2701 gatatgggag atacaaaagt tgtttcaaga ctgttagaag gtgagtattt caaatatgag
     2761 aatgttatac ctgaagaatt tgaaacgaaa ataagaataa ataaaaaaga attgcaagac
     2821 agtatagaaa gagcttcatt acttatttca agtgatgggt caagatatcc tgttactctg
     2881 ctcctgaagt cagacaaatt aattataact acaaatactc agataggaaa tgcaagagaa
     2941 gaaataaata ttgatatttg gggggatgag cttgaaataa agttcaatcc aagatatttt
     3001 atagattcac taaaggccat tgaagaggaa aatgttgatg taatgtttag ttccgatgta
     3061 ggtccttgca ttttgaaaag tgtagataaa gatactttta tatatatgat tttaccttta
     3121 aggaaataaa aaagaaggaa agaggtctta aagtatttgt atataaaaga tttaaaatta
     3181 aacaacttca ggaattataa ggaattatat tctgagttca atatggatta taatataatt
     3241 tgtgggaaaa atgcacaagg taaaacaaat attcttgaag ctattttttt gtgttcttca
     3301 ggaagatctc acagaacatc aaaagacaat gaattgatca aatttctgga aaatagttat
     3361 tatgtaaatg ttgatgtaaa tagaaaatat acagatacta ctatagagat atattatgag
     3421 aaggactcaa gaaaaaagat aaaaataaat agtgtaccgt ccaaaaaaaa cggagagtta
     3481 atgggacagc ttaatacagt tattttttca ccagaagata tgaatataat aaaaggtagt
     3541 ccctcagaaa gaagaaggta tattgatata gcaataagtc agcttaagcc atcttatttc
     3601 tataatttac aacaatattc aaaaatcatt gctcaaagaa actgcttatt gaaaacatta
     3661 aatataaaca atggtataaa aagtaaaatg tatgaaacac ttgatatatg gaatgaaaag
     3721 ctggtagaga caggaagcag aataatgatt gaaaggtata atttcttgaa ggagcttaat
     3781 gaaaaaataa aagaaaaaca tattaagtta acagggggaa gagaagaatt agagattaga
     3841 tatgctcctt ctattttgat tgaaaatgga ttggataaaa aaagtataga aaatagcttt
     3901 tataagaaat tggtatcatc agttgaaaga gaaattaaaa actgtgctac tctttatggc
     3961 cctcaaagag atgatatgga agtattagta aatgaaaaga atgttaaatt atttggatcc
     4021 cagggtcaac aaagaacagt tgttttatcg ctaaaacttt cagaggtgga tattgcatat
     4081 agattgataa atgaatatcc ggtactgctt ttggatgatg tgttttcgga gttggatata
     4141 aaaaggcagc aataccttat agaaagcatt aaaggaatgc aggtttttat tactactacg
     4201 gaaattaatt tcataaagac attaaaggaa aataataatt taagtttgta tatggttgaa
     4261 gagggagtag taaaggatac taatattata tagaaaagca atattaattg cataaaatca
     4321 tgtgttggta taaaataaat atagtttatt ataatgtatt atatatggaa tgggaatgga
     4381 gaggaatata tgtttttgca tataggtggt gatgtagtaa ttccaattag aaatattata
     4441 gctatctttg atttggaaaa aactaccata tccaaagata caaaagagtt tttgaaaact
     4501 gctgaagagg aagggtttat tgaaagtata tctgatgata tacctaagtc atttgttgta
     4561 actgagacag ataaaaaaag taagatatat ttatccccga tatcttccac tacattacaa
     4621 aaacgttcag ggtttataaa ggaaatgaca tttacaaaat aggccgataa atattaagtt
     4681 tataaataaa tataccggag agggaagtat gacagaaaat aatttaaaca aaattgaata
     4741 tgacgaaaat cagatacagg tattagaagg gcttgaggct gttagaaagc gtccgggaat
     4801 gtatatagga agtacatcga gtaaaggatt gcatcatctt gtttatgaaa ttgttgcaaa
     4861 cagcatagat gaggcgcttg caggtagatg tgacaaaata aagataactg ttgaaaagga
     4921 taattatatt acagttgaag ataacggaag cggaattcct gtagggcctc atccaaagtt
     4981 aggcattcca actattgaag ttgtacacac aatattacat gccggaggaa aatttggcgg
     5041 tggagcatat agtgtttcag gagggcttca tggtgtagga gcatctgttg taaatgccct
     5101 gtctgaatat atgattgtag aggtttcaag aaatggatat atttacagcc agaggtatga
     5161 aagaggaaaa gctgtaacaa aacttgagat aataggaaat actgaaaaca caggtacaaa
     5221 aacaacattt aaaccagatc cttttatatt tgaggaagta gtatttgact ttgaaggaat
     5281 gatatcaagg tatagagaaa tggctttctt gaataaggga ataaagatta tattaatcga
     5341 taacagacct gaggaaccgg tagagaagac gcttcattat gaaggtggta ttatctcttt
     5401 tgtagaatat attaacaaaa acaaagaggt aatccataaa cctccaatat atattgaagg
     5461 tggaaaggat tcaactgtag ttgaaattgc attgcaatat aatgacggat atgctgaaaa
     5521 cgtgtatagt tatgcaaatg atatcaacac aactgaggga ggaactcatt taacaggttt
     5581 tagatcaggt ataacaaaag ttataaatga ctatgcgagg aaatataaca tattaaaaga
     5641 atctgacaaa aatctgatgg gtgaagacgt aagggaagga cttactgcaa taataagtgt
     5701 taagctttta gaaccgcagt ttgaaggcca gacaaagaca aaactaggta acagtgaagt
     5761 gagaggtata gttgaaaatg tagtaagtga aaaacttgaa tattatttgg aggaaaatcc
     5821 agctgaagca agagccatta ttgaaaaatg tacaatggct gcaagagcca gagaagctgc
     5881 aagaaaagct agagagctga caagaagaaa aagtgcactg gaaagtactg ctcttcctgg
     5941 aaaattagca gattgctcag agaaagatcc ttctttatgt gaaatatata ttgttgaagg
     6001 agattcagcc ggaggatctg caaagcaggg aagagataga aaattccaag caatacttcc
     6061 tttatgggga aaaatgctta atgtcgaaaa agccagaata gatagagttt atggaaatga
     6121 taaactaatg cctgttatta ctgccctagg cacaggtatt ggggaagaat ttgatataga
     6181 gaaattaaga tatcataaag taataataat ggctgatgct gacgtagacg gttcgcatat
     6241 tagaatgctt ttacttactt ttttctttag atatttaaaa cctttagttg agaaagaata
     6301 tatttatatt gcacgtcctc ctctttataa aattacaaaa gggaaagagc atatgtatgc
     6361 ttacgatgac ttggatttag agaaaatagt aagagagaaa gaatgggaca aaaaagaatg
     6421 taccatacaa agatataaag gtttgggaga aatgagcgca gaacaattgt gggatacgac
     6481 aatgaatcct gaaacaagaa gcattattaa agttgaagta gaagatgctg tagctgctga
     6541 tgaaatattt tcagacctga tgggagagaa agttgaacca agaaagcaat atatacatca
     6601 gcatgcaaag gaagtagtaa atctggatat ttaaaatata agaaataaaa acataatatt
     6661 tataaaattg gcacaagaaa acctcatata tctaacttgg aggattgatt gtgccttaat
     6721 tttttaaact aaatacatga tttcatgtat ttatgaaatg atgatatttc atgtgagtat
     6781 ttaaagtttt tagagaaata tattacagaa gctatcgtta cgatatttag agccttctct
     6841 aacgcttctt ctgataattt cagcaaaaat taaatatgca cccatgcaaa attataacat
     6901 gtaatttctt taaaatgtat gatatactta aaataatgca atataaattt acttatatta
     6961 ctggtttgag tgcagagaaa taatagataa tgcactaaat ctggttttat gcaggacaat
     7021 catataatta attaggagtg aatttaatgg caaaaataat tgcaattgca aatcaaaaag
     7081 gtggagtagg gaaaacaaca actgctatta acttaagtgc atgtctggca tataagggta
     7141 aaaaagttat tctaattgat aatgatcctc aaggaaactc aaccagtggt ttgggatttg
     7201 aaaaaagaaa attgaagaaa tctacatacg atattcttat taatgatgaa gatatagaaa
     7261 atgttatttt gaaaacatgt atagataacc ttgaattatg tccttcaaat atacaattgg
     7321 cgggagctga aatagaattg gtaactgcat tttcacgtga aacaaggatg aaaaatgcaa
     7381 taaataaaat aaaagatagg aatgacttta taataattga ttgtcctcca tcattgggtt
     7441 tattgactat aaattcgcta actgctgcag atactatatt agtacctata caatgtgaat
     7501 attatgcgct agaaggatta agtcagctta tggatacggt aaagcttgta caaaggcact
     7561 taaataccga tttggatgta gaaggtgtag tattaacaat gtttgatgca aggacaaatt
     7621 tgtcaattca agtcgtagaa gaagtaaaaa aacattttag aaataaagta tatagaacag
     7681 taataccaag gaatgtaaga ctaagtgaag ccccaagtta tggattacct ataattttat
     7741 atgattcaaa atctaagggt gctgaatgct acatggacct tgctgatgaa gtaatagaat
     7801 gttcggaggg atttaataat gactaaaaaa agacttggaa gaggattgga tgctcttata
     7861 acaatagatg aaaatgaaaa tgatagtata aaagaaatga aaataaatga tattgagcca
     7921 aatactgctc aacccaggaa aaagttcgat gatgaaagtc ttaatgaact agctgaatct
     7981 ataaaaaaac atggagtaat acaacctata attattaaaa agcaaaatga tatgtataaa
     8041 attgttgccg gtgaaagaag atggagggca gcgagaattg caggattaga tagtattcct
     8101 gtaatagtta aggatttatc agagaaagaa attgttgaag tagctttaat agaaaatctt
     8161 caaagggaag acttgaatcc gatagaagag gcagaagctt atgaacaatt aattagtgag
     8221 tacgaaatga cgcaggaaga aatatcgcaa atagttggaa agagtaggtc ggctattgct
     8281 aatacaataa ggttgcttgg attgtgtgat gaaattaaaa ggaatattat tgatggaaaa
     8341 attacaagtg ggcatgcaag agctttatta acaatagaaa ataaggaagt ccagaaaaaa
     8401 cttatggaag aaataataaa aatgaattat aatgtaagag acacagaaaa gctggttaaa
     8461 aaatatgaaa attataaaga gacggaaaca aaaaatataa ataatttaaa gagtgaaatt
     8521 agaaaccagg aaattattga actagaggaa gttttaaaga atatactagg tacaaaggtg
     8581 aaattgaatg atgaaaagaa aaaaggtaaa attataatcg agtattattc aaatgatgaa
     8641 ctagacagaa ttgtagaatt aattagaaag atagaaaaac aataaaaaca agaatgtttc
     8701 acgtgaaaca ttcttgttaa ttgaaggtat aatag
//
LOCUS       DAOSDD010000195         8721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3001_length_8721_cov_6.055158, whole genome shotgun sequence.
ACCESSION   DAOSDD010000195 DAOSDD010000000
VERSION     DAOSDD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3001_length_8721_cov_6.055158"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1738)
                     /locus_tag="PK733_14780"
     CDS             complement(146..1738)
                     /locus_tag="PK733_14780"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOJ11840.1"
                     /translation="MEKINYQEFYRYDELVKFLENVSDNKKDFCKLISLADTRECRKL
                     YLLEVTDYSTGKAEGKSAYYVQANVHSNESAGTMAALHLINSLLYCDEYKELLKKVTF
                     YIVPRVNPDGAELALTTHAPIRSRLEATDRRNGLVPEDLNGDGMILNMRWEDPTGRFV
                     EDEIDKRIMVTRRPGDKGPFYKLHTEGIISNYDGTSITDGCRHFDFNRNYPENWDNRM
                     EVAGAYPFSEIETRAVGDFLISHPNIFAAIDLHCGTPAILRPSFLSDNDMDQADLSLI
                     LEFGKAAEKLIGFPLMRSSDYRQPWQKPDILPGNSIDWFYKNLGIFGYTIELGWGLSS
                     AGIFGNESFVADSRTRETTFMRKVLEFHDSKGSKIFTPWQEFEHPQLGKVEIGGLMTG
                     NARFMYPPDMEEISPRVTEFLTKHAEQHPELIICNIEKESPTEGIYRIRATVGNIGGL
                     STRVMNGGGGFEKKIPVKTRILSEDNIEILNQILVYEVDNLAALGDSYKQEWFIKGKP
                     GDNIIIETFQPKAGMKKVEIIL"
     gene            complement(1819..2694)
                     /locus_tag="PK733_14785"
     CDS             complement(1819..2694)
                     /locus_tag="PK733_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11841.1"
                     /translation="MKRTFGEKVFQFFDIIILLLIAASIIFPFINILSISFSTKEAVN
                     SRSVTFWPVGFNVTAYKLILNSNIFLRSLVNTIGLTIVGTIGSIMLTVMVSYALTKKF
                     PGKPFVTYYFILTMYFGGGLIPTYLLVSKYLNLKNTYWALLLPYFINVFYIIVIRSQI
                     DSIPVTIFDAAYIDGANEYQTLFNIVVPVIIPSIAAVSMFFALGKWNLWFPVLIYTDH
                     KKYWTLQYYLRSVFFERFINAKDQGFSLGDLVEIPEENIRMATIILAALPIVSIYPFI
                     QKYFVKGIISGAVKG"
     gene            complement(2728..3669)
                     /locus_tag="PK733_14790"
     CDS             complement(2728..3669)
                     /locus_tag="PK733_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11842.1"
                     /translation="MRFLHNIKNNAAIKISDKAKGVIPIYVLLLPAMILLFIFHYIPI
                     AGIMIAFKDYNPFQGILKSAWVGTKYFKLYFTDTNFWRVMKNTLVINVYQLIFGFPFP
                     IIFALLLNEIRANKFKKIVQTISYLPHFISWVVAAAITTAILSPVTGIVNVVLKNLFG
                     VEPIYFLVKKEYFRTILVITSTWKNFGMSAVYYIASLASIDQELYQAASIDGAGRLKQ
                     TWHITLPGLRNIIIVLLVINLGNILNIGFEQIFLLYNDMVYDVGDVISTYTYRIGLEG
                     GRYSLTTAIGLGQSVVNFLLVYSANKASRVVAGWSLW"
     gene            complement(3755..5389)
                     /locus_tag="PK733_14795"
     CDS             complement(3755..5389)
                     /locus_tag="PK733_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11843.1"
                     /translation="MNRYDLKRKLLVLLIITLVSAICLSACSNTTSTGKQNGKTAEQN
                     GSKEDGNVKTEPPAKISMFYQEAGQAFPDDFKHEDNWFINFLCEKANVEITELIVPTL
                     TDAETKFSLLMSSGRIPDLIERFSITELKDYGTKGAFLPSLDIIKSSPNISRFFDDSQ
                     LRAMQSENGIAYIIPVLPANDDFSVMQARWDLLEKVGYKEPPDTLDEWVEACRKLKKY
                     DPNSIPICSVGLNYAQFIFNPFNVGAGHGWNYFPERGKVSNVWEGDNIIKAVTFGKQL
                     YEEGIWDKEFVTNSGTEYGQKKINNNVLVQYNNYGGYTVWLNRYIIEGKTDYRLIPTR
                     QPMAEGVGIDQWYFAPTMLGPYAFAVSANTKEKEAVTRLLNVLYSDDVNMLSTYGREG
                     IEYKVENGKKTPIFPAASESSWKSIYGWAFVNNGDKLNYLIPTYIHSSKMSDSEKLDY
                     IELSSKATKKVKEISTGKLGYNPFAVADFIEEDLRNLATEVTEIQKSLITKAILNEIS
                     IDEFAVEKEKLVKKYQHITDAYNVVTEKSKTQYNIK"
     gene            complement(5662..7938)
                     /locus_tag="PK733_14800"
     CDS             complement(5662..7938)
                     /locus_tag="PK733_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11844.1"
                     /translation="MKNFIKIQKFIVHNFLSSLIIVVICTFIMSGVSYYTLHKNMLNW
                     VVNNNNKVLEQYSAMINNLIIANSEEIYTKILSDISSDERIKYFTHSPLINNLPGTIY
                     VRNYLSGIQESNPIIDSAEIFYIKNHLLVNGRDIKYDLFYEFRKNELQPYLDMQRNIN
                     SGNHSLYSVIDKNAISIIRPVAYGEENQALIILNYSISALRSYLSERPMPEFSSFYVV
                     GSDNSLIFTPEDINTAQEILKDKQVTQIFQYTSGNGTYTTKIKDTQSVVSYITYEKYN
                     WKYMSIAPISVFIQPITFILSNLLTSVIVTILAGLLLAALVCVYQSKPIRLIITLCEM
                     ANIKTNNKNITNTKDTYSIITGTLADMAKTIEKHNQDFSRIIPTLIDNFVVWFLSEEH
                     ISTDEIKNRMLHLNIRFPFANYCIIAIKAAIFITNDKTKNDEEYDQKYILAEMAFKLE
                     NLFKTNNSYISFLKKDDFLIGIINFDFSEERFGIICHELTETLIEDHYLYMSLSQVTQ
                     DICEIANISKYIIKNLDYSFIYPEKKHYILTQTDGLKINNLNTVKTYSDSFISCLKLH
                     NYHSALCEFDLLVNEIRNKEYDIDYIKVLLRNISLEIENMSNLNRTLVSELMKIFYCS
                     YNILALRSGLHGVITRHYLHHESSNASSDKLISNAKFHITSNLLDPMLTLQTVSDALN
                     VSSTYLSRIFPEKENVTFIEYITNAKMEFGRKLLLETELSINEISNKLNYSTPQYFIS
                     LFKKSFSVTPGNYRKNHK"
     gene            complement(8516..>8721)
                     /locus_tag="PK733_14805"
     CDS             complement(8516..>8721)
                     /locus_tag="PK733_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ11845.1"
                     /translation="IDIMSDIITKIIERRQELGISQRDLENLTGIRQEAICRIETMKN
                     VPQLDTLIKLMGPLGLKLSVVKQ"
BASE COUNT         2770 a         1600 c         1268 g         3083 t
ORIGIN      
        1 tgtacctgtt ttcagggaac cagaagctcg tcattttaat gacgatgtag ttcacagttc
       61 atacctattt taagaagaaa tatttttaat tatattgcta atccttgata ttcctaggtt
      121 taaaattcat gcttcaggtc tgcccttata aaattatttc aacctttttc attccggctt
      181 ttggttggaa tgtttcaatt attatattat cgcctggttt cccctttata aaccattcct
      241 gtttgtagga atctcctaaa gcagctaaat tatcgacttc atataccagt atttgattta
      301 aaatttcaat attgtcttct gataaaatcc ttgtctttac tggtattttt ttctcaaaac
      361 ctcccccacc attcattact ctggtagata gtccccctat gttgccaact gtcgccctta
      421 tccgataaat accttccgtt ggagattcct tttcaatatt acagataata agttccggat
      481 gctgctcagc atgcttagtt aagaactcgg tgactctcgg ggagatctct tccatatcag
      541 gtggatacat aaatctagca ttccctgtca ttaagcctcc tatttcaacc ttccctaact
      601 gtggatgttc aaattcctgc caaggagtaa atatttttga ccctttagag tcatgaaatt
      661 ccaaaacttt tctcataaat gttgtttctc tggttctgct atcagctaca aatgattcat
      721 ttccaaatat gcctgcagaa ctcaaccccc accctaattc aattgtatag ccgaatattc
      781 ctaaattctt ataaaaccaa tctattgagt tccctggcaa aatatcaggt ttttgccagg
      841 gctgcctata atcgcttgat ctcataaggg gaaaccctat taatttttct gctgcttttc
      901 cgaattcaag gatcagagaa agatccgctt ggtccatatc attatcactt aaaaatgaag
      961 gtcttaatat tgcaggggta ccgcagtgaa gatcaatggc tgcaaatata tttggatggg
     1021 atattagaaa atccccaact gctctcgtct caatttcgct aaaggggtat gctcctgcaa
     1081 cttccatccg gttatcccaa ttttccggat aattcctgtt aaaatcgaaa tgccgacaac
     1141 catcggttat acttgtgcca tcataattgc ttataatccc ctcggtatgt aatttataaa
     1201 aaggtccttt atctccgggc cttcttgtaa ccattattct cttgtcaatt tcatcttcaa
     1261 caaaacgccc tgtcggatcc tcccaacgca tatttaaaat catcccatcg ccattaagat
     1321 cctctggcac taaaccattt cttctgtcag tcgcctccaa tcggcttctt atcggtgcat
     1381 gcgttgtcaa tgcaagctca gcgccatcag ggttaaccct tggaactata taaaatgtaa
     1441 ccttttttaa caattcttta tattcatcac agtataatag tgaatttata aggtgtaatg
     1501 ccgccatggt tcctgcactt tcatttgaat gcacattggc ctgaacgtaa tatgcacttt
     1561 ttccctcggc tttacctgtc gaataatcgg taacctcaag taaatataac ttcctgcatt
     1621 ctcttgtatc tgcaaggcta attagcttgc agaaatcctt tttattatct gaaacgtttt
     1681 ctaaaaattt taccaactca tcatacctgt aaaactcttg ataattaatc ttttccaagg
     1741 aaacacaccc cttcaaaata taaaattaag attacttaaa taaatttatt tttacatcat
     1801 tgaaaaattg cattgcattc atcccttaac tgcgcctgaa ataattcctt ttacgaagta
     1861 tttttgaata aacggataaa ttgatacaat aggcaaagca gccaggataa ttgttgccat
     1921 acgaatattt tcttcaggaa tctctactaa atctcctaat gaaaagcctt gatcttttgc
     1981 gttaatgaat ctttcaaaaa acacactcct taggtaatat tgcaatgtcc aatatttctt
     2041 gtgatccgta tatatcaaca cagggaacca caaattccat tttccgaggg caaaaaacat
     2101 agaaacagct gctatagaag gtattattac gggaacaaca atattaaaaa gagtctggta
     2161 ttcattagca ccatctatat acgctgcatc aaaaatagtt actggtatac tatcaatttg
     2221 gctacgtata acaattatat aaaagacatt tataaaataa ggtaataaca atgcccaata
     2281 agtatttttt aaattaaggt atttacttac caataaatag gtaggaataa ggcccccacc
     2341 gaaatacatg gttaggataa agtagtaagt tacgaaaggt ttgccgggaa atttttttgt
     2401 tagagcataa gaaaccataa cagtaagcat tattgaacct atcgtgccaa cgatggttaa
     2461 tcctattgta ttgacaagag agcgtaaaaa aatattacta ttaaggatta gcttatatgc
     2521 tgtaacatta aagccaactg gccaaaaagt aacactccta ctattaacag cctctttggt
     2581 gctgaatgaa atacttaata tgtttataaa tgggaatatt attgaagcgg caattaataa
     2641 taatataata atatcaaaaa attgaaaaac cttttcgcca aaagttcttt tcaatttaca
     2701 cacctacctt tgctgaaatt tgagtatcta ccataatgac catcctgcga caacgcgtga
     2761 tgctttgttc gctgaataaa caagcaaaaa gtttactact gactgtccta acccaatagc
     2821 agttgtcaat gaataccttc ccccctcaag accaatccta tatgtatatg tgctaatgac
     2881 atctcctaca tcataaacca tatcattata taataaaaat atttgctcaa accctatatt
     2941 taatatatta cctagattaa taactaatag tacaatgata atatttcgaa gacccggcaa
     3001 tgtgatatgc catgtttgtt taagccgtcc tgccccatca attgaagcag cttgatataa
     3061 ttcttgatct atggaagcaa gagatgcaat ataatagact gcagacattc caaagttctt
     3121 ccaagttgaa gttataacaa gtattgttct gaaatattct ttttttacta aaaaataaat
     3181 tggttcaacg ccaaatagat tttttagaac tacattcaca atccccgtaa ccggagaaag
     3241 tatagctgtc gtaatagcag ccgcaacaac ccaagatata aaatgaggaa gataacttat
     3301 agtctgaaca atcttcttaa atttgttagc tctgatttcg ttcaacaaca gtgcaaaaat
     3361 tataggaaat ggaaacccga aaatcaattg atatacattt attactaaag tattcttcat
     3421 aactctccaa aaatttgtgt ctgtgaaata aagtttgaaa tatttcgtcc cgacccaagc
     3481 actttttaag attccctgaa atggattata gtctttgaaa gctatcatta tccctgctat
     3541 tggaatgtaa tgaaaaataa aaagtaatat catggcaggc aaaagcaaaa cataaattgg
     3601 tattactcct tttgctttat cgctgatttt tattgcagca ttattcttta tattatgtaa
     3661 gaatctcaaa tctcatgcct ccaaactata ttttcgggga aatgcattaa tatttacatt
     3721 tcccctttaa gattttgatt gaatggctta tatactactt tatattatat tgtgtctttg
     3781 atttttcagt aacaacatta tatgcatccg ttatatgctg atattttttt actagttttt
     3841 ctttctcaac agcgaattcg tcgatactta tttcatttaa aattgccttt gttataagac
     3901 ttttctggat ctccgtaact tcagtcgcta aattcctaag atcttcttca ataaaatcag
     3961 caactgcaaa aggattatat cctaattttc ctgttgatat ttctttaacc ttttttgtcg
     4021 ctttagatga aagttcaatg taatcaagtt tttctgaatc actcattttt gatgaatgta
     4081 tatatgtagg aattaaatag ttaagtttat ccccattatt tacaaatgcc caaccatata
     4141 tggatttcca agagctttca gatgcagctg ggaaaattgg agtcttttta ccattttcaa
     4201 ctttatattc aattccctcg cggccatagg tagacagcat atttacgtca tcgctatata
     4261 aaacattcag gagccttgta acggcttcct tttctttcgt atttgcacta actgcaaaag
     4321 catatggacc caacatagtg ggggcgaaat accactgatc tatacctaca ccttctgcca
     4381 ttggttgtct tgtaggtatt aacctataat cagttttacc ttcaatgatg tacctgttta
     4441 gccagacagt atagccacca taattattgt actgcaccaa tacgttattg ttaatctttt
     4501 tttgcccata ttccgtaccg gagtttgtga caaattcttt atcccaaatg ccttcttcgt
     4561 ataattgttt accgaaagta acagccttaa taatattatc accttcccat acattagaga
     4621 cttttcctct ctctgggaag taattccagc catgaccagc ccctacatta aaaggattaa
     4681 aaataaattg agcgtaattt aacccaactg aacatatagg tatagaatta gggtcgtatt
     4741 tttttaattt gcggcatgct tcaacccatt catctaatgt gtccggtggt tctttatatc
     4801 cgactttctc caacagatcc caccttgcct gcattacact aaaatcatcg ttagccggta
     4861 gcactgggat aatgtacgca ataccgtttt ctgactgcat agcacgaagt tgagaatcgt
     4921 caaagaacct tgaaatattt ggggaacttt tgataatatc caaagaaggt agaaaagccc
     4981 ctttagtacc ataatctttt agctcagtaa tgctaaacct ttcaattaaa tcaggaattc
     5041 ttcctgaaga catcaaaaga ctaaactttg tttcagcatc agttaatgtt ggcacaatta
     5101 gctcggtaat ttcaacattt gctttttcac atagaaaatt aataaaccaa ttgtcttcat
     5161 gcttaaagtc atcaggaaaa gcctgcccag cctcttggta gaacatacta atctttgcgg
     5221 gtggttcagt cttaacatta ccatcttcct tgctgccgtt ttgttcagca gtttttccat
     5281 tttgcttacc tgtgcttgtt gtattactac atgcgcttaa acagattgct gaaactaatg
     5341 taataattag caaaaccagc aacttccttt ttaaatcata acgattcata atgtgctgcc
     5401 tcctttaaaa ttttatggtt ctgatatcct catcctatcg aaaatttttg tagttgcaat
     5461 aatatctttt taacagcaaa ttattagtaa taaaaaagca tatccttttc gtaaccccgt
     5521 ccctatttag gaataagcgt tttggttctt gtatcagtca tttcaccgat cttaaagaat
     5581 taatacaaaa ccgagctaat atttatagcc agcgataaag ttatgttttt ttaaacataa
     5641 tgatattttg tacctaagaa attatttgtg gttttttcta taattaccgg gggttactga
     5701 aaaacttttt ttaaacagac taataaaata ctggggtgtc gaataattga gtttgtttga
     5761 aatctcatta atactcagtt ctgtttctag caataattta cgtccgaatt ccattttagc
     5821 attcgtaata tactctatga atgtaacatt ttccttttca ggaaagattc ggcttaaata
     5881 cgtactgctt acattaagag catctgaaac agtttgcaat gtcaacatcg gatcaagcag
     5941 attggaggtt atatgaaatt ttgcatttga tataagctta tctgaggagg cgttgctgct
     6001 ttcatgatgc aaataatgtc ttgtaataac gccatggagc ccacttctta atgccaaaat
     6061 attatagctg caataaaata ttttcattaa ttcgctcact aaagttctgt tcaaattgct
     6121 catgttttcg atttcaagag aaatattacg cagcaaaact tttatataat ctatatcata
     6181 ttctttattt cgtatttcat ttaccaataa atcaaattca cacaaagcac tatggtaatt
     6241 atgaagtttt aagcaagata tgaagctatc tgaatatgtc ttcactgtat ttaagttgtt
     6301 tattttaaga ccatcggttt gtgttagaat gtaatgcttc ttttcaggat aaatgaaact
     6361 gtaatctaga tttttaatga tgtactttga aatattggcg atttcacaaa tatcttgtgt
     6421 aacttggctt aatgacatgt ataaataatg atcttctatt aacgtttcgg ttagctcatg
     6481 gcagataatc ccaaaacgct cttcactgaa atcaaagtta attatgccga ttaagaaatc
     6541 atcctttttc aaaaagctta tataggaatt attagtttta aataaatttt caagcttaaa
     6601 ggccatttca gcaagaatat acttttggtc atattcttca tcatttttgg tcttatcatt
     6661 agtaataaaa atagcggctt taattgcaat aatacaataa tttgcaaaag ggaatctgat
     6721 atttaaatgt aacattctat ttttaatttc atcggtactg atatgttcct ccgataaaaa
     6781 ccaaaccaca aaattatcaa taagggttgg tatgatcctc gaaaaatctt gattatgttt
     6841 ttctatggtt tttgccatat ctgctagagt gccggttata atgctgtaag tatcttttgt
     6901 attagtgata ttcttattgt tggttttaat atttgccatt tcgcataaag taataatcaa
     6961 tctgattggt tttgattggt atacacaaac taatgccgct aatagcaaac ctgctaaaat
     7021 tgttactatg acagaggtca gaagattgct taagataaag gtaatcggct gaataaacac
     7081 acttataggt gcaatcgaca tatacttcca attatatttt tcataagtta tatatgaaac
     7141 aacactttgg gtatctttaa tctttgtagt ataagtgcca ttccctgaag tgtattgaaa
     7201 aatctgcgta acctgtttat ctttaagaat ttcttgggcc gtattgatat cttctggagt
     7261 gaaaattaaa gaattatcag atccgactac ataaaaagaa ctaaattccg gcattggtct
     7321 ttcgcttaag taactcctta acgcactgat tgaataattt aatattataa gggcttgatt
     7381 ttcttctccg taagctacgg gtcttataat gcttatagca tttttgtcta tgacactata
     7441 taaagaatga ttaccggaat tgatatttcg ctgcatgtca aggtagggtt gcagctcatt
     7501 tttgcgaaat tcgtagaata gatcatattt tatatccctg ccatttacga gtaaatgatt
     7561 ctttatgtag aatatttccg cagaatctat aatagggttt gattcttgta taccggataa
     7621 atagtttctg acatatatag tcccaggcaa attgtttatt aaaggcgaat gtgtaaaata
     7681 tttgatacgt tcgtcagatg atatatcgct taatatttta gtataaattt cttctgagtt
     7741 agcaataatt aaattattaa tcatcgctga atattgttcc aatactttat tattattgtt
     7801 tacaacccag ttcagcatgt ttttatgaag agtataataa gaaactccac tcattataaa
     7861 agtgcaaatt accactatga tcaaagaaga taaaaaatta tgcactataa atttttgaat
     7921 ctttataaaa ttcttcataa atttatacat ccctttttgt aattctttcc cataagtctg
     7981 tagaaccaca aattgttttg gcaaaaaatc ccttttatgt aataaagctt acaaaagaag
     8041 ctcattcaca ctttaataat atgataagat tatttacgtt attttgcaag ttaaagttta
     8101 atcaccttcc tatagtctaa ccctttgtag tatccatcat aatacccttt atgaaatact
     8161 tttgtaggat aaggtacaaa atcagtactg gaacacttgc aactatttca taatattgtg
     8221 tttttctaaa ataatccaca tgcttttcgt atttttatat acaattcgta tttttcatat
     8281 acaaacggct tgcctattga taatagttcc tttcactcat agcagacgat aatatgttcc
     8341 atttctttag tgtatctatg tgtttcaata agtttttcat ttaaaaatta ccaacctttt
     8401 ttaaaacaat ctactcttaa gccaatttgt atattatctt gatttaggaa acacagtgag
     8461 aatcctattc attttaataa agcagatgaa agccgtgttt cgtgaaaatg cattattatt
     8521 gttttactac agataatttt aatccaaggg gccccattaa cttgattaat gtatccagtt
     8581 gaggaacatt tttcatagtt tctattctgc atattgcttc ttgtcttatt cctgtaaggt
     8641 tttccaaatc tctctgtgat attccgagtt cttgccgtct ttcaataatc ttagttatta
     8701 tatcagacat aatgtcaatt t
//
LOCUS       DAOSDD010000196         8631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3050_length_8631_cov_5.470499, whole genome shotgun sequence.
ACCESSION   DAOSDD010000196 DAOSDD010000000
VERSION     DAOSDD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3050_length_8631_cov_5.470499"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..909)
                     /locus_tag="PK733_14810"
     CDS             complement(121..909)
                     /locus_tag="PK733_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ11846.1"
                     /translation="MKYSFMSFSTPELNLDQSLNIARLYGYDGFEPRTSAGHKHGIEI
                     GSTKPVLREARLKSEDSGIKICCIATSCCFANPSNNEGNIELAKRSIDLASEVNSPAI
                     RIFGGNIPEGVSREKSFDLIVEAMMKLSNYALERGVTICIETHDSWCDPKAVADIIQS
                     VNHPAIAVNWDIMHPALTASYTIEKAFEILKPWIKHVHVHDGLRTDKGLEFKTISQGK
                     VDHKTAIRLLKDSEYNGFISGEWIGWEHYEIHLPRELKILKSFE"
     gene            1350..2201
                     /locus_tag="PK733_14815"
     CDS             1350..2201
                     /locus_tag="PK733_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ11847.1"
                     /translation="MTNKDDRFEKILDTDFSAVHHIDDSYAMNIFHFHNVFEIYFAQT
                     GGLKYFVDNRIYPVEKNDLFIFNHLDLHRIGLPPGIHYERLIIIFNPDYIKSLCTDTT
                     NLLECFVNRSPNFCHRSPLSNEQAEAYMSLFHKAQSHMETGEYGDDVLKKTALAGILV
                     FVNRIYRDNHYSLPMRYDAEYDKVKPILAYIDDNIHQKLSLDHLSDYFYISKFHLCKI
                     FKEVTGFTVNEYIVYRRIIKAAELLRKNFLVSQVSEMTGFQNDCHFITTFKKIVGITP
                     KQYAMKK"
     gene            complement(2484..2882)
                     /locus_tag="PK733_14820"
     CDS             complement(2484..2882)
                     /locus_tag="PK733_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11848.1"
                     /translation="MKYLKLIRYLKLTNCCLFDSTRNREPDSVEIAALATVLYSFYDG
                     VEKIFLSIAKYVDDFVPVENKWHKALLIQMTKKTDKRNAVITSETISVLTEYMGFRHF
                     YRHAYSFQLSWSKLKDLFNRANAKLLTHCK"
     gene            complement(3384..4865)
                     /locus_tag="PK733_14825"
     CDS             complement(3384..4865)
                     /locus_tag="PK733_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOJ11849.1"
                     /translation="MTNVPEVKLGIVAVSRDCFLVSLSEGRRNAVAEACEKQGVSIMQ
                     ATTVVENEKDVVKALKEIEDAGVNALVVYLGNFGPEGPETLLSQKFNGPAMYVAAAEE
                     TETNLIQGRGDAFCGMLNASYNLGLRKLNPYIPEYPVGTPDEVADMIYEFRDIARVIL
                     GIKNLKVFSFGPRPQDFLACNAPIKPLYDLGVELMENSELDLFESFNNHSADPRIPDV
                     VKEMEEELGAGNNFPGIIPKLAQYELTLKDWMDKNIGISKYAVFANKCWPAFQTQFGF
                     VPCYVNSRFASKGIPIACETDIYGALSEYIITCASDLPATLLDINNTVPKDMYDNNKE
                     IIGDYCHNDMFMGFHCGNTPSCCMKNCAMRYQLIMHRLLEPDNEPNITRGTLEGNIRP
                     GDITLFRLQGTADCKLRSYVAQGEVLDIDSKSFGGIGVFAVKEMARFYRHVLIGKRYP
                     HHAGVGFRHIGKVLFAAMNMLGIEDIAFNQPSNMLYKGENPFR"
     gene            complement(5018..6706)
                     /gene="araB"
                     /locus_tag="PK733_14830"
     CDS             complement(5018..6706)
                     /gene="araB"
                     /locus_tag="PK733_14830"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOJ11850.1"
                     /translation="MSRKKYAIGVDFGTQSGRALIVDVSSGEEIADDVMEYPHGVMDE
                     YLPDKNTKLEHDWALQHPQDYLNVLKTTIRNVLSKSGISNEDVIGLGVDFTACTMLPV
                     DSNGEPLCLRKELENVPHSYVKLWKHHAAQDEANEMNRIAVERSEEFLKRYGGKISSE
                     WLIPKIWQVLNEAPEIYNLADKFIEAADWVIMKLTGNERRNSCTAGYKAMWNKRKGYP
                     SKDFFKTLDSRLENVVDDKLSRDIYPIGAKAGEITREAAKLTGLKPGTAVAVSNVDAH
                     VALPAVGITEPGKMLMIMGTSTCHMVLDTEEKIVPGMCGVVEDGIIPGYFGYEAGQSC
                     VGDHFEWFIKNCVPEQYMIEARERGINIHMLLREKANKMKPGQSGLIALDWWNGNRSV
                     LVDVDLTGIIIGYTLLTKPEEIYRALIEATAYGTRMIIETFEENGVPVKELYAAGGIA
                     EKDEFMMQIYSDVTNREIKISGSSQAPALGSAMFGAVAAGKERGGYDSIVEAAGVMAK
                     VKDKYYKPNSKNVKVYDKLYAEYKQLHDYFGRGENDVMKRLKEIKRQEALLDAK"
     gene            complement(6833..7906)
                     /locus_tag="PK733_14835"
     CDS             complement(6833..7906)
                     /locus_tag="PK733_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11851.1"
                     /translation="MATNEPKYQRVKLYIKELIREGVICNGQMLPSEYELMEMFAVSR
                     HTVRQAFGELAKEGWVYKEQGRGTFANYNKNIDHKGIIGVITTYISDYIFPHIISGIE
                     EVLAKGGYVLALANTNNNKEKEAQHIMNFMEQNIVGLIIEPTRSAEENTNLHLLKEVE
                     SRDIKTIFINAAYPDYDSPYIIMDDVKGGFTATQHLLQTGHRKIAGIFKKDDLQGLKR
                     EEGYKKALKSFNITPDESIIGHYGTESKGTYPYIFAQKLVESRERPDAIVCYNDEIAI
                     KVVQAIRDRGLEIPDDISIIGYDDSALALASDIKLTTIRHPKKQMGEQAAHYLIEMLE
                     NNIHKPVMTYEPELIVRSSCKSL"
     gene            8171..>8631
                     /locus_tag="PK733_14840"
     CDS             8171..>8631
                     /locus_tag="PK733_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ11852.1"
                     /translation="MAGVSGATVSRVFNNPDTVSQLTREKVFQAAKELNYHPNVIASN
                     FTKGVSGNIGVIVPRIPNVRIFSVYYFTELLNGIGDALSEEGYNLVLFYHNLESNYLR
                     ADYLGPDSANIASTNISSTNESLAYKFSPAGRLSSKHVVTGGDYSRYFLGG"
BASE COUNT         2583 a         1798 c         1361 g         2889 t
ORIGIN      
        1 caggatcaaa acttttttga tgaaaaagtt tttatgggct taaattcatt ttagctgcgc
       61 gtgcaattta ttttttcata attcttgtgc aaaaaatatg cacaattgat ttgactacaa
      121 ttactcaaaa ctcttcagaa tcttaagctc acgaggcaga tgaatttcat agtgttccca
      181 gccaatccat tccccgctta taaagccgtt atattctgaa tccttcagga gtcttattgc
      241 ggttttgtga tccactttgc cctggcttat agttttaaac tccagtccct tatcagtcct
      301 taatccatca tgtacatgca catgttttat ccagggtttt aatatttcaa aagctttttc
      361 tatagtatag gatgctgtaa gtgccggatg cattatatcc cagtttacag ctatagcggg
      421 atgatttaca ctttgaataa tatccgcaac tgctttggga tcacaccagc tgtcatgggt
      481 ttcaatacaa attgttacac ctctctccaa tgcataattc gaaagtttca tcatggcttc
      541 aacaatcaag tcaaaactct tttctctgct gacaccttcc ggtatatttc cgccaaatat
      601 cctgattgca ggtgaattga cttcagaagc cagatcaatt gagcgttttg ccaattcgat
      661 atttccttca ttatttgaag gatttgcaaa gcagcaggag gttgctatac aacaaatctt
      721 tatcccgctg tcttcagact tcagtctagc ctcacgcaaa accggttttg tggagcctat
      781 ctctatacca tgtttatgtc cggcacttgt acgaggctca aaaccatcat acccataaag
      841 ccttgcaatg ttaagggatt ggtccaaatt aagttctggg gtggaaaagc tcataaatga
      901 atacttcata ataattcact cctattcata gtgaaaatag caaattaaat ctgtctggga
      961 gccttggaca aaagtctcaa tattgcaaaa taaccttgca taagttgtgt ttgtttccca
     1021 agtcgataca gcatattgtt gtcaaatttg cttaatttct acttttgtct atggctccca
     1081 gatcagcatt aatgtcttat actttagtgt cttattgtat aatgccttct ttttttagta
     1141 tatatttaaa cgcaaagaaa atatttaaga atattggcaa aaagtttgca tatcttgcta
     1201 ttataaggta aaattgattc tactgcataa actctgattt cgatgcaaaa ggcgaatgaa
     1261 gcatggaaac accattgatg gtgtgaaaag cgctgctgaa acacatggga gcatttaaca
     1321 aatataaatg taaaatggag atgaatacaa tgacgaacaa ggatgataga tttgagaaga
     1381 tattggacac agatttctct gcagttcatc atattgatga ttcctacgcc atgaatatct
     1441 ttcattttca taatgttttt gaaatatatt ttgctcaaac aggaggactt aaatactttg
     1501 tagacaatag gatatatccc gtagaaaaaa atgatctttt tatttttaac caccttgacc
     1561 ttcatagaat aggactgcct cccggaatac attatgaacg ccttattatt attttcaatc
     1621 cggattatat taaaagttta tgcaccgata ctacaaatct gctagagtgt tttgtaaaca
     1681 gaagtccgaa tttttgccat cgctcgcctt tatcaaatga acaggcagaa gcatatatgt
     1741 ccttatttca taaagctcag agccacatgg aaaccggtga atatggagat gacgtcttaa
     1801 aaaaaacagc tcttgccggg atacttgttt ttgttaaccg catataccgg gataatcatt
     1861 actcgcttcc tatgcgttat gacgcggagt acgacaaagt taagcctatt ttagcatata
     1921 tcgatgataa tatacatcaa aagctatcac ttgaccatct gagtgattat ttttatataa
     1981 gcaagtttca tttatgcaaa atatttaagg aggttacagg gttcactgta aatgaatata
     2041 tagtataccg aaggattata aaagctgccg aattgctgag gaaaaacttt ttagtctctc
     2101 aggtgtctga aatgacagga tttcaaaatg actgtcattt cataacaacc ttcaaaaaaa
     2161 ttgtgggtat taccccaaaa caatatgcaa tgaaaaagta aggtagaaca agtcaacctg
     2221 actcgtgctc tttatatatt ttaaaggaat aactctgttc gaatgtttca aatatattac
     2281 cacttattgc tccaaactgt aattgctcgg aagtgtacca gcccataaga taactagcta
     2341 atcctgtgcc cataaagtat atctttgggg tttttacgat tctcttgtta aaattgttgt
     2401 aatatggctg aggtaaatat ataattcctg aaatcctaag tattgatacc cggttcttaa
     2461 tggtatctac cgatttaccg atatcatttg caatgcgtga gtagtttggc atttgccctg
     2521 ttgaataagt ctttcaattt actccatgat aattgaaaag aatatgcgtg tctataaaaa
     2581 tgtctaaaac ccatatattc agttaaaact gaaattgttt cagaagtaat tacagcattt
     2641 cttttatctg ttttcttagt catttgtata agcagtgcct tatgccattt attttctacc
     2701 ggtacaaaat catctacata cttagctata cttaggaaaa ttttttcaac gccatcatag
     2761 aaagagtata ataccgtcgc taacgcagct atttctactg aatcaggctc tctatttcta
     2821 gtactatcaa acaaacaaca atttgtaagt tttaaatagc gtatcaattt cagatatttc
     2881 aaattctatt cttttaatag cttcagagta cacgatgcat atcatccttt ccaattagca
     2941 gctttgaaaa attggattca tcatctaagt ctattaaatc aatatttctt tcaagattaa
     3001 taagcagttg cccgtatacc ctgaaaaatc tctccggtat aagtcctctt actgcaatat
     3061 caatatctga atttgatgaa taattacctt tgaaaagaga accaaaaata tatgcttcta
     3121 cacacccttc atttttttat aatgtttaat gtatttcaac atcaacaatg tattcactag
     3181 gaatagaaaa catactccca ccaccttaat tgttattttt attatatgct aaataatgga
     3241 tttttcctaa tagtagtata acatacaaac aacatctttg aatagctttt tcatgataga
     3301 tagtgccaac tgcagcctca catgctaaac gcccatattt atctgcagtt ttatgcccta
     3361 tttgccatgg gccatccatt tcattaccta aacgggttct cacccttata aagcatattt
     3421 gatggttgat taaatgcaat atcctctata cccaacatat tcatggctgc aaataacact
     3481 ttacctatgt gcctaaaccc aactcctgca tgatgaggat atctcttccc aataagcaca
     3541 tgcctgtaaa atctggccat ttcctttaca gcgaaaactc ctatcccgcc aaaagattta
     3601 gaatcaatat caaggacttc accctgtgct acatagctcc taagtttgca gtcagcagtt
     3661 ccttgaagtc tgaaaagagt tatgtccccc ggtcttatgt tcccttccag ggttcctctg
     3721 gttatatttg gctcattatc cggctcaaga agcctgtgca taataagctg gtacctcata
     3781 gcacaattct tcatgcagca acttggtgta ttgccgcagt gaaaacccat aaacatgtca
     3841 ttatggcaat aatcccctat aatttcctta ttattatcat acatatcctt aggcactgta
     3901 ttgtttatat caagtagggt tgcaggaaga tccgaagcac aagtaattat gtattcacta
     3961 agagcaccgt atatgtctgt ttcacatgct ataggtatac cttttgaagc aaaccttgag
     4021 ttaacataac atggcacgaa tccaaactgt gtctggaatg caggccaaca tttgtttgca
     4081 aatacagcat atttagaaat tccaatgttt ttatccatcc agtctttgag agtaagttca
     4141 tattgagcaa gttttggtat tattcccgga aaattattac cagctccaag ttcctcctcc
     4201 atttccttta ccacatcagg aatgcgagga tctgctgaat gattattaaa gctttcaaac
     4261 aagtccaatt ctgaattctc cattaattct acacccaggt cataaagagg ctttataggc
     4321 gcattacatg ccagaaagtc ctggggtctt ggtccaaagc taaatacctt tagatttttt
     4381 ataccaagta taaccctggc aatatcccga aactcataaa tcatatcggc aacttcatca
     4441 ggggttccca cagggtattc aggaatatat ggattaagct ttcttaagcc cagattgtat
     4501 gatgcattca acataccgca aaaagcatcg ccacggcctt gtataagatt tgtttcagtt
     4561 tcctctgcgg ctgcaacata cattgcaggt ccgttaaatt tctgggaaag taaggtttca
     4621 ggtccttcag gtccgaaatt tccaagataa accaccaagg catttacacc tgcatcttct
     4681 atctctttta atgctttaac cacatctttt tcattttcta caaccgtagt agcctgcatt
     4741 attgaaacac cttgtttttc acaggcttct gctactgcat ttcttctccc ttcactaaga
     4801 gaaactagaa agcaatctct gctcaccgct actataccaa gtttcacttc tggaacattg
     4861 gtcatatata cacctcccaa aatttgacac ctctatacaa acataatatt catatgaata
     4921 tttatttaat atcaattgaa aacttattta acatcaatat aaggaatatt ttaatgcctt
     4981 acggacacag cccatcactt tctgtttctg gttcctatca tttagcatcc agtaaggctt
     5041 cctgccgctt tatttctttc agcctcttca ttacatcatt ttctccacgg ccaaaataat
     5101 cgtgcagctg tttatactca gcatacaact tatcatatac ctttacattt ttggaattag
     5161 gtttataata tttatctttt acctttgcca ttacaccggc tgcttcaaca atactgtcat
     5221 atcctcctct ttccttacct gctgccacag ctccaaacat agcagaccct agtgcaggag
     5281 cttgtgaaga tcctgagatt ttgatttccc tgttagttac atcagaataa atttgcatca
     5341 tgaattcatc cttttcagct atgcctcctg cagcatataa ttcctttacg gggacaccat
     5401 tttcctcaaa ggtttctatg atcatacgag ttccataagc agtagcttca attagagcac
     5461 gataaatctc ttcaggttta gtaagcagtg tgtatccaat aattattccg gttagatcta
     5521 catcaactaa tactgaacgg ttgccattcc accagtcaag tgctatcaac ccactctggc
     5581 ctggtttcat cttgttggct ttttctctaa gaagcatgtg aatgtttatt cctctttctc
     5641 ttgcttctat catatattgt tcaggtacac aatttttaat aaaccactca aaatggtctc
     5701 caacacatga ctgacctgct tcatatccaa aatagccagg tataatgcca tcttctacga
     5761 ccccgcacat gccgggaact attttctctt cagtatccag taccatgtgg caagttgaag
     5821 tgcccataat cataagcatt ttccccggct ctgtaatgcc aacagcaggt aatgccacat
     5881 gggcgtctac attggaaact gcaacagcag ttccaggctt cagacctgtt aatttggcag
     5941 cctccctggt aatttcaccg gcttttgcac ctatgggata aatgtccctg ctcaatttgt
     6001 catccactac attctcaagt cttgaatcaa gagttttaaa gaaatcttta gatggatatc
     6061 ccttacgctt attccacata gccttatatc ccgctgtaca gctatttctc cgttcattcc
     6121 ctgtaagctt catgataacc caatcagcag cttcaataaa tttatctgca agattgtaaa
     6181 tttcaggtgc ctcatttaaa acctgccata tcttgggtat gagccattcg gaagatatct
     6241 tgccgccata ccttttaaga aattcttcac tacgctcaac agctattctg ttcatctcat
     6301 tggcttcatc ttgggctgca tggtgtttcc agagctttac ataactatga ggcacatttt
     6361 caagctcttt ccttaagcat agtggttcac cattgctgtc aacagggagc attgtacagg
     6421 ctgtaaaatc tactcccaga cctataacat cttcattgga aatgcccgac tttgaaagca
     6481 catttctaat tgtggtctta agcacattaa ggtaatcctg tggatgctgc aaagcccaat
     6541 catgctccaa ctttgtgttt ttatcaggca gatattcatc catcaccccg tgaggatatt
     6601 ccataacatc gtcggctatt tcttctcctg aagatacatc aactataaga gctctgcccg
     6661 actgtgttcc aaaatctacc ccaattgcat acttttttct gctcatattt aaccccctct
     6721 atatttttct agtattatct ttctatataa tgcaaaaata attttatatg gaacaaatta
     6781 gttttttgcg tattcacaaa tatcaatttg tttcacattt taaataatga ccttacaaac
     6841 tcttgcagga tgacctgaca attagttcag gctcataggt cattacaggc ttatgtatat
     6901 tattctccag catttcaata agataatgtg cagcctgttc ccccatttgc tttttcggat
     6961 gccttattgt tgtaagtttt atatccgaag caagtgccag agcagagtca tcgtatccta
     7021 ttatggaaat atcatccggt atttccaaac ctctatcccg gatcgcctga acaaccttaa
     7081 tagctatttc atcgttataa catactatag catcaggtct ttcccggctt tcaacaagtt
     7141 tctgggcaaa tatataagga taagttcctt tactctcagt gccgtagtga cctataatac
     7201 tttcgtctgg tgtgatatta aagcttttca atgctttttt atatccttct tcccgcttta
     7261 acccctggag atcatctttt ttaaaaatac ctgcaatttt cctgtgacct gtttgaagta
     7321 aatgctgagt ggcagtaaac ccacctttca catcatccat aattatatat ggactgtcat
     7381 aatctggata cgcagcattt ataaatatgg ttttaatatc tctggattca acctccttca
     7441 aaaggtgaag attcgtattt tcttctgcac ttctggtagg ttcaattatt aagcccacta
     7501 tattttgttc cataaaattc attatatgtt gagcttcttt ttccttgtta ttgttggtgt
     7561 ttgcaagagc aagtacatat cctccttttg caagcacctc ttcaattccg cttattatat
     7621 gaggaaagat gtaatctgat atgtatgtag taattacacc tataatacct ttatgatcaa
     7681 tgtttttatt atagtttgca aaagtacccc tgccttgttc cttataaacc cagccttcct
     7741 ttgcaagttc accaaatgcc tgccttacag tatgacgact tactgcaaac atttccataa
     7801 gttcatattc agaaggaagc atttgaccgt tacaaataac gccttctcta atcaactctt
     7861 ttatatataa ttttaccctc tgatatttgg gttcattagt tgccatatta agcatatata
     7921 tcctttcggc caacttgtac gtacatgtaa attgtagcat aagcgaattt atgtgtcaat
     7981 tgttattagt actttttgtc aaaattattg acttcatata taaaaagagt tacaatgtac
     8041 acgtgtacac cagtatatat aaatgaaagc aacatcattt gataatacta aaccgagtta
     8101 atgagcatgt acaccaatgt ataaatgaga gggggcgatt attgtaacaa ccagaaatga
     8161 tgtggctaaa atggcaggtg tgtctggtgc tacagtttcc agagttttta ataaccctga
     8221 taccgtatct caattaactc gtgaaaaagt ttttcaggcg gcaaaagagc taaattatca
     8281 tccaaatgtt atagcaagca actttacaaa aggagtaagc ggaaatatag gggttatagt
     8341 accacgaatt cccaatgttc gcatattttc agtctattat tttactgaac tcctgaacgg
     8401 aataggagac gctctttcag aagaaggata taatcttgtt ttattttatc ataatttaga
     8461 gtcgaattat ttacgagcag attatttagg accggattcc gccaatatag cctcaacaaa
     8521 tatttcgagt acgaacgaat ctctggctta caaattctcg ccagcaggta gattatcatc
     8581 aaagcatgta gttaccggtg gagattattc tagatacttc ctgggcggaa a
//
LOCUS       DAOSDD010000197         8491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3113_length_8491_cov_5.028450, whole genome shotgun sequence.
ACCESSION   DAOSDD010000197 DAOSDD010000000
VERSION     DAOSDD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3113_length_8491_cov_5.028450"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK733_14845"
     CDS             <1..741
                     /locus_tag="PK733_14845"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOJ11853.1"
                     /translation="LLSFFPFLIFLLTLLSYTKITDGNILKNYSHLIPNSAYNIIMDI
                     IDQAAKARSETLLSFSMLATIWAASIGVSAITRGMNKAYRQKESRSFWKVKGLALLFT
                     LLIALVLVLTLVMLVLGETLGNYLFSQLGISRFFSYTWQVARHVVPLLAMFFLLLNIN
                     RYIPDYKLSFKDVVPGVAVSTAGWIILSLVFSYYVNNYAQYPKTYGHIGGVIALLIWL
                     YWSNVVILVGGEINAAILLKRRGNLDNK"
     gene            894..1778
                     /gene="speE"
                     /locus_tag="PK733_14850"
     CDS             894..1778
                     /gene="speE"
                     /locus_tag="PK733_14850"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOJ11854.1"
                     /translation="MNNNLYPEYIKKINNELWLTEFEAEGLKFSYKLKEVIYSKQSDF
                     QHIMILDSYDFGKMLVLDGIVQTTARDGHVYNEMISHIPMNIHPHPKRILIIGGGDCG
                     AVREVTKYQCVEQIDFVEIDELVVRACLEYLPEVSGNLSDPRVNFIFQDGIKFVQNIV
                     REEDYYDIVIVDSSDPVGPAIPLFSLDFYKSIYKILRDDGLMVCQSESPVFYIETLKQ
                     TYQRLSSVFPITKVYDAVVPTYPGGAWTFTLGSKKYSDPMPWKSNKDTNKDTKYVNDE
                     ILEKCFALPKYIKKELKK"
     gene            complement(1920..3956)
                     /locus_tag="PK733_14855"
     CDS             complement(1920..3956)
                     /locus_tag="PK733_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ11855.1"
                     /translation="MGVNLKEYLEEKLIIEYAKGEEEWVDIYITPLNRIDVTIVSDIA
                     DRLDIGEIGKFIKEIVREYSGQEGNKYSIGFLDTYNIEEADDLALRRPETKKTPITWS
                     DVISLNKRLKRKAADRPYSVICFYSYKGGVGRTTALVQIAYNLAKQGRNLVLIDFDIE
                     APSFHLLFKEWIDNSDTGIEHGLVDYLYERMVNVDPRDYKIKISDIFVPIKFNEAPEG
                     NIYVIPATTKLTYQYIFKLAQLQSKIIYENDYMEDLIKSLEENLKFDTVFIDTRTGIN
                     QWGAFSLLGFADQIMLIVSPNEENIEGLSNIIDLMKKAGLDNYVVAMSKFEGDSAGIK
                     LAKHYFKKLTGAEQEFIGIGYIPSIAMTGKYPFVDMPEPYRALSDYILECQIVKFIIE
                     FIKQIDVAEILSQISQSMDIDKGIGICAGAGIGYCAEEAGIGHSTDADTNICINTCKN
                     TCKNTCIGVETDTCKGDTLKAELNEDNIKNLIIQHIISHINPALFKWYAYAMLENGSI
                     SRNLEGMEFIDAGRVDNNKEIDSNNFMCEKDSYSKRIESLIKSEIFGQRVLDLFWGIR
                     VNPKRYSKTMISWFYEELVKHGDTNSSMVIDIINKAIYHEMDKIHQHIDSNDDTNNDN
                     INDSNNGSNSDNNNDSNNNSNNGSNNDSSNYNNNYSKCRDRLLSIESIRKAFDN"
     gene            complement(4024..4557)
                     /locus_tag="PK733_14860"
     CDS             complement(4024..4557)
                     /locus_tag="PK733_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11856.1"
                     /translation="MAMARDVENYEKAYIDRRKDFTIMRKGNRKVMTMYLGGIYLECL
                     LKTVIIKKNKIEKSIAVFENRQRIIYWYSNSNYEKLKNMGKVRSEDYNDLKDGFNPEH
                     NLILALKQIDELNTTLPAEGVRRLEMLNKPVNNQSFTSLRYTYDEQIPDSLYNEWEEN
                     FIYFMNFFNRMKKTLIF"
     gene            complement(4808..5449)
                     /locus_tag="PK733_14865"
     CDS             complement(4808..5449)
                     /locus_tag="PK733_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOJ11857.1"
                     /translation="MRIPDHIGIIPDGNRRWAERRGLPKEKGYEEGIDPGMSLFKLCR
                     KEGVKELTYYGFTADNTKRPAEQRIAFTDACINAVRQLTNEDASLLVVGNTNSPMFPK
                     ELLPFTTRKTFGNGGIKINFLVNYSWKWDLDYLKRSNSMSNNIANQIETCDISRVDLI
                     IRWGGRRRLSGFLPVQSIYADFYVIDDYWPDFKSDHFYDALNWYSEQDITLGG"
     gene            complement(5551..6030)
                     /gene="rlmH"
                     /locus_tag="PK733_14870"
     CDS             complement(5551..6030)
                     /gene="rlmH"
                     /locus_tag="PK733_14870"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOJ11858.1"
                     /translation="MKVTIIAVGKIKENYLKEGIKEYSKRLSRYCNLEIIEVPDQQAP
                     DNLSPVQEEQVKIVEGDGILKKIKTGTIVVALDIKGMKLGSEDFADKINKLAISGSSH
                     ITFIIGGSLGLDHRITDMADIRLSLSDMTFPHQLTRLILLEQIYRVFKIINGETYHK"
     gene            complement(6168..6968)
                     /locus_tag="PK733_14875"
     CDS             complement(6168..6968)
                     /locus_tag="PK733_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ11859.1"
                     /translation="MIKFCSLYSGSSGNAIFISNERARILIDAGLSGKRIAEALISIG
                     EDPAGLSAILVTHEHNDHIKGVGVISKRYDIPIYANELTWQYLQSNITNISKINHKNI
                     KYFDNNREFEICGIGVKAFSIPHDANDPVGFCFYSEGKKITIATDIGHITSELTESLD
                     GSDLLLLESNHDVGMLKVGPYPWWLKQRILGEKGHLSNEVAGEVIADMAENGTRFFFL
                     GHLSRENNFPELAYQTVSNALTERKICIGTDITLDVARRDCASRVVCM"
     gene            complement(7007..8266)
                     /locus_tag="PK733_14880"
     CDS             complement(7007..8266)
                     /locus_tag="PK733_14880"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ11860.1"
                     /translation="MEKYIIHGQKPLIGDVHISGSKNAAVAIIPAALLVAGKCRIENV
                     PDIGDVKALMDILVNLGAEVEYEDENTLAINAENVNTYIATYEMVKSLRASYYIIGAL
                     LGRFGHAEVPFPGGCDFGYRPIDQHIKGFEALGAEVSIEHGIIKAKAERLTGASIYLD
                     VVSVGATINLMLAAVKAEGVTVIENAAKEPHVVDVANFLNAMGANIRGAGTDVIKIKG
                     VDRLNGGTTHSIIPDQIEAGTFMIAGAANGNCITVKNVIPKHMESLTAKLTEMGVKVE
                     VIEDSIRVTGAGKILRANIKTLPYPGFPTDLHPQTAALLCLAGGTSIITEGIWDSRFQ
                     YVEELKRMGAAIKVEGRVAVIEGVKNLTGAPTKAIDLRAGAALVIASLLAKGKSEISN
                     INYIDRGYENLEFKLKALGANIKRVSV"
BASE COUNT         2494 a         1761 c         1293 g         2943 t
ORIGIN      
        1 ctgctgtctt tttttccctt tttaatattt cttttaacat tactaagtta tacaaaaata
       61 acggatggca atatcctaaa gaactactcc catcttatac caaatagtgc atataatata
      121 ataatggata tcattgatca ggcagcaaaa gcaaggagtg aaaccctgct ttcctttagc
      181 atgttggcaa caatatgggc tgcgtctatc ggagtatcag caattacccg aggaatgaac
      241 aaggcttata ggcagaaaga aagccggtct ttctggaaag ttaaaggtct ggccttatta
      301 tttactctct taattgcgtt agttttagtt cttactcttg ttatgctggt tctaggcgaa
      361 acccttggca attatttgtt ttcacagctg ggaatttcaa gatttttcag ctatacttgg
      421 caggttgcaa gacatgtagt acctcttctg gctatgtttt ttttgttact taatatcaat
      481 cgctacatac ctgattacaa gctctcgttt aaggatgttg taccaggagt ggcagtttca
      541 actgcaggat ggataatttt atccttggtt ttttcctatt atgttaataa ttatgcacaa
      601 taccctaaaa cttatggtca tattggcggt gttatagctc tccttatatg gctttattgg
      661 agcaatgtag taatattggt aggtggtgag attaacgcag caatactatt aaaaagaaga
      721 ggtaatctag ataataagta agaaggctgg gggaattcct gccaaaaagt ttgacatcta
      781 ataaaagaag ggttttacct cgcatttgtc gaagtagtat catatgttat tgtaagcgga
      841 ataaaaagta ataataagca aaaaaataac aaaaccaaca taggagagta aaattgaata
      901 ataatctata tccagagtac attaaaaaga tcaataatga gttatggctt acagaatttg
      961 aagccgaagg tctaaaattc tcatataagc taaaagaggt tatctattca aagcaatctg
     1021 attttcaaca tataatgatt ctcgattctt atgatttcgg caaaatgctg gtgcttgatg
     1081 gtatagttca gactacagcc agagatgggc atgtttacaa tgaaatgatt tctcatatac
     1141 cgatgaatat acatccccac ccaaaacgca tattaattat tggtggtgga gattgcggtg
     1201 cagtaagaga agtaactaaa taccagtgcg ttgaacagat tgactttgtt gagatcgatg
     1261 agcttgtagt aagagcatgt ttagaatacc ttcccgaggt ttcaggcaat ctttccgacc
     1321 ccagggtaaa tttcattttt caggatggca ttaaatttgt acaaaatatt gtccgggaag
     1381 aggactatta cgacattgta attgtagatt cttcagaccc tgttggtccg gcaatacctt
     1441 tgtttagcct ggacttctat aaaagcattt acaaaatact aagagatgat ggtttaatgg
     1501 tctgccaaag tgaatccccc gtattttaca ttgaaacttt aaaacaaacc tatcagcgtt
     1561 tgagcagtgt atttcctata acaaaggtct acgatgcagt cgttccaaca tacccgggag
     1621 gagcatggac ttttacactg ggttcaaaaa aatattcaga cccgatgcca tggaaatcta
     1681 ataaggatac aaataaggat acaaaatatg taaatgatga aattttggag aaatgctttg
     1741 ccttaccgaa atatataaag aaagaactaa aaaaatagaa atttcaactt cactgatgtc
     1801 agttgcgtca gttgtattga tgatttaata cctggatgct attcatagtt aaagcattgt
     1861 attactgaag gctaaaacat tctattcctg gcggctgaga acataaagaa aggtctgagt
     1921 taattatcaa aggcctttct aatgctttct atactcaaaa gtctatccct gcatttactg
     1981 taattgttat tataattgct gctatcatta ttgctaccgt tattgctatt attattgcta
     2041 tcattattgt tatcactatt gctaccatta ttgctatcat taatattatc attattagta
     2101 tcatcgttgc tatcaatatg ttgatgtatt ttgtccattt cgtgataaat tgctttattt
     2161 attatatcta taaccatact gctgtttgta tctccatgct tgaccaattc ttcatagaac
     2221 catgaaatca tagttttact gtagcgtttt ggatttactc taattcccca gaacaagtca
     2281 agtactctct gtccgaaaat ttcacttttt atcaaacttt cgatacgctt gctataggaa
     2341 tctttttcac acatgaaatt attactgtct atttccttat tattatcaac gcgtcctgca
     2401 tcaataaatt ccatcccttc taagttgcgg gaaatactcc cattttcaag catagcataa
     2461 gcataccact taaataaagc aggatttata tgactgataa tatgttggat aatcagattt
     2521 tttatattat cttcattcaa ctctgctttt agagtatctc ctttacaggt atccgtctca
     2581 acacctatac aggtgttttt acaggtattt ttacaggtgt ttatacagat gtttgtatca
     2641 gcatcggtac tatgtccaat gccagcttct tctgcgcaat atcctatacc tgcccctgca
     2701 catatcccta tacctttgtc tatgtccatg ctctgtgata tttggcttaa aatttcagca
     2761 acatctattt gttttataaa ttcaataata aatttaacta tttggcattc gagtatatag
     2821 tcacttaatg ctctgtaagg ttccggcata tccacaaatg gatatttccc cgtcattgca
     2881 atagaaggaa tatatcctat gccgataaac tcctgctctg cacctgttaa ctttttgaaa
     2941 tagtgttttg caagttttat tcccgcgcta tccccttcaa attttgacat tgctactaca
     3001 tagttgtcca aacccgcctt tttcattaaa tcaataatat ttgacaaacc ctctatattt
     3061 tcttcattag gagagacaat caacattatc tgatccgcaa agccaagcaa agaaaaagct
     3121 ccccactgat taatacctgt cctggtatct ataaaaacag tatcaaattt aagattttct
     3181 tccagacttt taataagatc ctccatatag tcattctcat agataatttt cgactgtaat
     3241 tgtgcaagtt taaagatata ttgatatgta agttttgttg ttgcaggaat aacataaata
     3301 ttgccttccg gagcttcatt aaattttatg ggaacaaata tatcagatat ttttatttta
     3361 taatctctcg ggtctacatt taccattctt tcataaagat aatccacaag gccatgttct
     3421 atgccagtat ccgagttatc tatccattct ttaaaaagaa ggtgaaaact cggagcttct
     3481 atatcaaaat caattaaaac aagattcctg ccttgttttg caaggttata tgctatctgt
     3541 accagagctg ttgtacgtcc tacccctcct ttataggaat aaaaacaaat tacgctgtag
     3601 ggcctgtcgg cagcttttct tttaagccgc ttgttcaaac ttattacatc cgaccaggtg
     3661 attggtgttt tcttggtttc aggcctgcgt aaagcaagat catctgcttc ttcaatgtta
     3721 taggtatcaa ggaaaccaat gctatattta ttaccttcct ggccggaata ttcccggaca
     3781 atttctttta taaattttcc tatttcacca atatccaacc tgtcggcaat atcactgaca
     3841 attgttacat caattctatt taggggggta atataaatat caacccactc ttcttcgcct
     3901 tttgcatatt caattatcaa tttttcttcc agatattctt ttaaattaac acccaattac
     3961 tcattcctcc agaaataatc ttgtaaaaag ttcagtgtta aaatccagtc aaatattcac
     4021 gtcttaaaat atcagtgttt ttttcattct attaaaaaaa ttcataaagt atatgaaatt
     4081 ttcttcccat tcattatata ggctatcagg tatttgttca tcatacgtat atctcaagct
     4141 tgtaaaactt tggttattta caggcttatt cagcatttcc agtctcctta ccccttctgc
     4201 aggtaaagtg gtattaagtt catcaatttg ctttaatgct aaaatcaagt tatgctcggg
     4261 gttaaatcca tccttaagat cattataatc ttcgctcctg actttcccca tatttttgag
     4321 tttctcataa tttgaattgc tataccagta aattattctc tgtctatttt caaatacagc
     4381 tatagatttt tctattttat tttttttaat tatcacggtt ttcaagaggc attccaaata
     4441 gataccacca agatacatag tcattacttt tctgttgcct tttctcataa ttgtaaagtc
     4501 ttttctccgg tcaatatagg ctttttcgta attttcaaca tctctagcca ttgccatact
     4561 ggtcaaaacc ccacttattt taatcttata aacattaatg cacctggatg ctttcccctt
     4621 cccttactac cattattata tcatgaattt cgaagaaatt gcatgatata attgtgcatg
     4681 tgcgtttttt gtgaagcaaa aataatcgca cgcacataaa ttccgccgga ggcatataat
     4741 atcctcatct gtgaggatat tatatcacaa ataaatgatt tgtcattaag tgctctacct
     4801 acctgcccta cccgccaagg gttatatcct gctcgctgta ccaatttaat gcatcgtaga
     4861 aatggtcgga tttaaaatcc ggccaatagt catctattac atagaaatct gcatatatgg
     4921 attgaaccgg gagaaagcca ctgagccttc ttctgccacc ccaacgtata ataaggtcaa
     4981 ccctcgaaat atcacaggtt tcaatttgat ttgcaatatt attgctcata gaatttgatc
     5041 tctttaaata atctaaatcc catttccagc tatagttcac taaaaagtta atctttattc
     5101 caccattacc aaatgtcttc ctggtagtaa atgggagcaa ttctttggga aacatggggg
     5161 aattagtgtt tcctacaacc agcaaagatg catcttcatt agttagctgc cttacagcat
     5221 taatgcaagc atcggtaaaa gctattctct gttctgctgg tcttttggta ttatctgctg
     5281 taaatccata gtaagtaagt tccttaactc cttccttcct acacagctta aaaagtgaca
     5341 tccccgggtc aattccctcc tcatacccct tttcctttgg caaccctctt ctttcagccc
     5401 acctcctgtt tccatcagga attatcccta tgtgatcagg tattctcata taaaccacct
     5461 caaatatttt gtaatattat tattagcatt tatcgtggaa ctataagtga gcaataatgc
     5521 ttaacaatat taatttaaga gtaacccaag ttacttgtgg taagtctccc catttataat
     5581 cttaaatacc ctgtatattt gctctaataa tataagccga gtaagttgat ggggaaaagt
     5641 catatcggat aatgaaagcc ttatatctgc catatcagtt attctatggt caagtccaag
     5701 agagcctcct ataataaatg taatatgtga gctgcctgat atagcaagct tattaatttt
     5761 atcagcaaaa tcctcagaac ctaatttcat gccctttata tccagggcaa caacaatagt
     5821 cccggtctta atctttttta agataccgtc gccttcaacg atttttacct gctcttcctg
     5881 tacaggactt agattatcag gtgcttgttg atcaggcact tctattattt ccagattaca
     5941 atatcgggaa agccttttgg aatactcttt gatgccttcc ttaagataat tctctttaat
     6001 tttgcccact gcaattatag ttaccttcac ttcgacttcc cacttccaaa cttgagaaat
     6061 atcctttttt cttttttagg aacatcctat tcatctaaac ttgagaaatg tcccctcttt
     6121 tctcttttct cgccatgcag gttatccctc ttgtacttct ccttattcta catgcagact
     6181 actctgcttg cacagtccct tcttgccaca tcaagggtta tatctgtgcc tatacatatt
     6241 ttcctttcag taagtgcatt gctaactgtc tgatatgcca gttcagggaa attattctcc
     6301 ctgctcaaat gtcccaggaa gaaaaacctg gtaccattct cagccatatc tgctataact
     6361 tcaccggcaa cttcattgga aagatgccct ttttctccta gaattctctg cttaagccac
     6421 cagggatatg gacctacctt cagcatacca acatcatgat tcgactccag caaaagaagg
     6481 tcactgccat caagagattc tgtcagttcg gaagtaatat ggccaatatc tgtggctata
     6541 gtaatttttt tgccttctga gtagaaacaa aaacctacag gatcatttgc atcatgaggt
     6601 attgaaaatg cctttacccc aatcccgcaa atttcgaatt ccctgttatt gtcaaaatat
     6661 tttatatttt tatgattaat cttgcttata tttgtaatat tactttgcag atactgccat
     6721 gtcaattcat ttgcatatat tggtatgtca tatctttttg atattacacc cacgcccttt
     6781 atatgatcat tatgttcatg agttaccaga attgcactca aacccgcagg gtcttccccg
     6841 atagaaataa gagcctcagc tattctttta ccgcttaggc ccgcatcaat caatattcta
     6901 gctctttcat tactaataaa aattgcattc ccgctgcttc cactatatag actgcaaaac
     6961 tttatcattt gtaacccccg gtttttccgg gtatacataa acccacttac acgcttaccc
     7021 tctttatatt tgcaccaagt gccttgagtt taaactccag gttttcatac cctctgtcaa
     7081 tataattaat attacttatt tctgatttgc cctttgctaa aagtgatgca attaccaacg
     7141 cagcacccgc ccttaagtct atggcttttg ttggagcacc tgtaaggttt ttaactccct
     7201 caattactgc tactctgcct tcaaccttta ttgcagcacc cattcttttc agctcttcca
     7261 catattgaaa cctggaatcc caaattcctt cagtgataat gctggtacct ccagccaaac
     7321 acagtaaagc tgcagtttgc ggatgaagat cagttgggaa gccgggatat ggaagagttt
     7381 ttatattcgc tctcaatatc ttgcctgcac ctgtaaccct gatactatct tcaattacct
     7441 ccacttttac acccatttca gttaacttgg ctgtaagtga ttccatatgc tttggtatta
     7501 cattttttac tgtaatacag ttgccatttg cggcacccgc tatcataaaa gtacctgctt
     7561 ctatttgatc aggtattata gaatgagttg ttcctccgtt taacctatca acacctttta
     7621 ttttaattac atcagtacct gcgcctctta tgttggcacc catagcattg agaaagttgg
     7681 ctacatcaac cacatggggt tcttttgcag cattttcaat cacagtaaca ccttcagcct
     7741 ttactgctgc aagcattaaa tttattgtag ccccaacact aactacatct aaataaatag
     7801 aagcacctgt aagtctttcc gcttttgctt ttattatgcc gtgctctatt gacacttctg
     7861 ctccaagtgc ctcaaatcct tttatatgtt ggtctatggg gcggtaacca aaatcacagc
     7921 ctcccggaaa aggaacctca gcatgcccaa atctgccaag aagggctcct atgatgtaat
     7981 aagatgccct taagcttttt accatttcat atgtagctat atatgtatta acgttttcgg
     8041 cattaattgc cagagtattc tcatcttcat attcaacctc agcacccaga tttactaata
     8101 tatccataag tgctttcaca tcaccaatat caggtacatt ctctatcctg cacttccctg
     8161 ctacaagcaa tgctgcaggt attatggcca cagcggcatt tttcgagccg cttatgtgga
     8221 catcaccaat taaaggcttt tgcccatgaa ttatatattt ttccattcgc cgtccaccta
     8281 tcttgttctt catttgttta atgatttatc cgaccaaacc atatatgtca ttaatcagcc
     8341 tgccggcata taaatattat accgttacaa atattattct actataaata taataccatt
     8401 atgaatatta tattgctata aatattatac tgttattata ccatgcaggt aaattttcac
     8461 accctggtca ttgtcgttat atgctattaa t
//
LOCUS       DAOSDD010000198         8471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3119_length_8471_cov_4.931797, whole genome shotgun sequence.
ACCESSION   DAOSDD010000198 DAOSDD010000000
VERSION     DAOSDD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3119_length_8471_cov_4.931797"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK733_14885"
     CDS             complement(<1..220)
                     /locus_tag="PK733_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ11861.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITKVLGMQGKANY
                     TAYDQIDKAPEEKERGITISTSHVEYETE"
     gene            complement(410..2475)
                     /gene="fusA"
                     /locus_tag="PK733_14890"
                     /pseudo
     CDS             complement(410..2475)
                     /gene="fusA"
                     /locus_tag="PK733_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(2564..3034)
                     /gene="rpsG"
                     /locus_tag="PK733_14895"
     CDS             complement(2564..3034)
                     /gene="rpsG"
                     /locus_tag="PK733_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ11862.1"
                     /translation="MPRKGHVPKREILPDPIYNDKIVTKLVNQIMQDGKKGVAQKICY
                     DAFDIINNKTGKDPMEVFEQAINNIMPVLEVKARRVGGATYQVPMEVRPERRQTLGIR
                     WLVSYARSRGERTMKERLAGEIMDAVNGLGGAFKKKEDTHKMAEANRAFAHYRW"
     gene            complement(3120..3548)
                     /gene="rpsL"
                     /locus_tag="PK733_14900"
     CDS             complement(3120..3548)
                     /gene="rpsL"
                     /locus_tag="PK733_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ11863.1"
                     /translation="MPTFNQLVKQGRKDILEKSNSPALQRGFNSLKKKPTEINSPQKR
                     GVCTVVKTTTPKKPNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTLDSAGVANRKQSRSKYGAKRPKAGAAKK"
     gene            complement(3572..3814)
                     /locus_tag="PK733_14905"
     CDS             complement(3572..3814)
                     /locus_tag="PK733_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOJ11864.1"
                     /translation="MLELLKNSKKTVGSKQSLKAIQGDQVKMCIIARDADEKVIRSIK
                     EPCEKKSIEITYADDMKKLGKACNIDVGASVVCILK"
     gene            complement(4079..7618)
                     /gene="rpoC"
                     /locus_tag="PK733_14910"
     CDS             complement(4079..7618)
                     /gene="rpoC"
                     /locus_tag="PK733_14910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOJ11865.1"
                     /translation="MLELGTFDAIRIGLASPEKIREWSKGEVKKPETINYRTLKPERD
                     GLFCERIFGPTKDWECHCGKYKRIRYKGIVCDRCGVEVTKQKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRSLEKVLYFASYIVLDPGQTPLFKKQILSEKEYRDSVE
                     KFGNKFKAGMGAEAIKELLAEIDLEELSKELKAEFDETSGQKRMRTIKRLEVVEAFRM
                     SGNRPEWMILDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKLYQCGLPKEMALELFKPFVMKKLVNDGLAHNIKSAKRMVER
                     VRDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPILVEGRAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFMMLSANNLLKPQDGKPVTVPTQDMVLGTYYLTVENQD
                     YKGKIRAFSSPEEAIMAYNQSQQDLIYETSIDNEDRLTLHAPIKVKITKQFDGKPVSK
                     IISTTVGKLLFNEAIPQDLGFIDRSVPENLFSLEIGFLVDRKALQKIIYRCIRVHGTT
                     KTAIVLDKIKALGFKYSTRGAITIGITDMIIPEIKKRYVAETEQKIENITKQYKRGLI
                     SENERYNAVISAWTESGENITKALMDNLDKFNPLYMMSTSGARGNINQIKQLAGMRGL
                     MADTQGKTIEIPIKSNFREGLNVLEYFISTHGTRKGLADTALRTADSGYLTRRLVDVS
                     QDVIIREFDCGTGHGFEVSDITEGGQVIEHLAERITGRYALENVINPQNGQVIIEAGK
                     MITDIEAEKVENAGIKKVKIRSILTCHSAYGVCAKCYGANLATGGEVDIGESVGIIAA
                     QSIGEPGTQLTMRTFHTGGVAGEDITQGLPRVEELFEARKPKGLAVISEIAGLVKISE
                     TKKKREVIVSNDGGESKSYMIPYGSRIKVMEGDYVEAGDELTEGSVNPHDILKIKGLI
                     GVQSYLLHEVQKVYRLQGVDINDKHIEIIIRQMLRKIKVEDAGDTNLLPGSLVDMFEY
                     ERENETALKDGLKTAEGKHALLGITKASLATESFLSAASFQETTRVLTDAAIKGKVDP
                     LVGLKENVIIGKLIPAGTGMKKYRDVTITTGSK"
     gene            complement(7643..>8471)
                     /locus_tag="PK733_14915"
     CDS             complement(7643..>8471)
                     /locus_tag="PK733_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ11866.1"
                     /translation="TPVFDGANEHDIMETLKTANLNEDGKTILYDGRTGEPFDNRVTV
                     GYMYILKLAHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAY
                     TLQEILTVKSDDVVGRVKTYEAIVKGENVPEPGIPESFKVLIKELQSLALDVKVYSEE
                     NEEVSIKESIDDDIDDLNVNMEGREDEEPLSDYDEGDDVLDNELELDDFDLESPESSG
                     NTDDDDDIISNLFLETDDFENDDIDPESVSFEDLDDLVDLGDLDEVNDELDDDDDNI"
BASE COUNT         2250 a         1971 c         1277 g         2973 t
ORIGIN      
        1 tttctgtttc atattcaacg tgtgaagtag aaattgttat tcctctttcc ttttcttcag
       61 gagccttgtc aatctgatca tatgcagtgt aattggcttt tccctgcata cctaatactt
      121 ttgttattgc agctgttaaa gatgtcttgc catggtctac gtgacctatt gtaccgatgt
      181 taacatgcgg ctttgtcctt tcaaacttag cctttgccat tgtataactc ctccttcatt
      241 ccggtaatcc aaccggcaat cattttttta ttgttaattt tattgttaat atattttttt
      301 actctgtaac cagttgaact acaggttttc gagtaatata ttactgcttt tacattttac
      361 atataaatct actttatata taaaattaaa ctagaataaa tactttatgt caatacctga
      421 ttaaattttc ttggatgttt tttggaactt cctcgtaatg gtggaactgc attgtaaagg
      481 ttccacggcc ctgggtcttt gaacgcaact cagttgcata tccaaacatt tctgccagag
      541 gtacataaga agttacaaca tggattccat ctctggcatc aattccctca attctgcccc
      601 tttttgagtt taaatctcca attacatcgc ccaaatattc atcaggtacc accacatcga
      661 ccttcataac aggttccagc aaaatcggac ctgccttgcg gcatccatct ttaaatgcca
      721 tagaaccagc tattttgaaa gccatttccg aggaatccac ctcgtgataa gaaccgtcaa
      781 ccagggttac ttttacatta agaacctgat aacctcctaa aataccacta ttcatggcat
      841 cccgtatacc ggcatcaata gcaggaatat attctttggg tattgaacct cctactatct
      901 tgtttacaaa tgtgtatcct gcatcgggtt cgttctgagg ttctagttct attacacagt
      961 ggccatactg tcctcgtcct cctgactgac gtacaaattt gccttcagat ttaacagctt
     1021 ttgttattgt ttctttgtaa gctacctgtg gtttacctac atttgcttca actttaaact
     1081 cccttaagag tctatctact ataatgtcaa gatgaagttc gcccatccct tcaattatag
     1141 tctgtcctgt ttcagcatta atactggctc tgaatgtagg atcctcttcc gacaattttt
     1201 gcagtgcaat acttaactta tcctgtcctg ctttagactt gggttctata gctactgaaa
     1261 taaccggttc tggaaattcc attgactcca gtaatatggg gtgatcctca tcgcatagtg
     1321 tatcacctgt tgaagtattc tttaaaccaa ctacacctgc tatgtcacca gtataaactt
     1381 catcaacgtc ttcacggtga ttggcatgca tcaacaggat cctgcccatt ctttctcttt
     1441 tatcctttgt agagttcaat acatgagcac ctgattttat ggtgcctgaa taaactctaa
     1501 aaaaacataa tttccctaca tagggatctg tcattatctt aaatgcaagt gcagaaaacg
     1561 ggcttttatc atcggcgtat ctttcatctt cttcgccatt attcaatatc ccttaaccgg
     1621 tggtatatca agaggcgaag gcaaataatc cacaatagca tccagtagat tttgaacccc
     1681 tttgttttta taagaagagc cacatgtaac aggaataatt tttactccta ttgtaccttt
     1741 tcttatgcat gcacgcaact cgtcttcggt tatttcttct ccctcgaggt attttactaa
     1801 aatatcctca tcttgttcag caacagcttc gatcaatttt tcacgatatt cttttacaat
     1861 atcaatcata taatcaggaa ttggaccctc ttccctttct tgtcccaaat catctttata
     1921 gattattgca tgatttttta caaggtcaat tatcccccga aaattctctt cttttccaat
     1981 tggtaactga atagggacag ggtttgcttt caatctgtcc ttaatcattt gtacaactct
     2041 gaagaaatcg gctcccatga tatccatttt atttacatga gcaatacgtg gaacatggta
     2101 tttatctgcc tgcctccaaa cagtttctgt ctgaggctca actcctcctt ttgcacaaaa
     2161 aagtgttact gaaccatcga gaacgcgaag tgaccgctcc acctcaaccg tgaagtcaac
     2221 gtgccctggc gtatctatta tattaattcg cttacccttc cattgggcgg tagtagcagc
     2281 agaggttatt gtaattcctc tttcctgctc ttgctccatc cagtccattg ttgcagaacc
     2341 ttcatgggtt tctcccattt tgtggagctt ccctgtatag aacaaaatac gttctgttgt
     2401 agtagttttt cctgcatcta tatgagccat tattcctata tttcttgtat cttccaaact
     2461 aaaccgcctg ggcattgcta tcctcctttc atttcacgat agttcactaa ataaacaaaa
     2521 tctataatta tataaatata taaccataat atatttattt atattaccat ctgtaatgag
     2581 caaaagctct gtttgcctct gccatcttat gagtgtcttc ctttttctta aatgcgcctc
     2641 ccagaccatt tacagcatcc atgatttctc ctgcaagtct ttccttcatt gttctttctc
     2701 ctctggaacg tgcataactt accaaccatc taatgcctaa tgtctgcctt ctctcaggcc
     2761 ttacttccat tggaacctga taggtagctc cgccaactct ccttgcttta acttcaagaa
     2821 ccggcattat attattaata gcctgctcaa aaacttccat agggtctttg cctgtcttat
     2881 tatttattat atcaaaagca tcgtaacaga ttttctgagc aactcccttt ttaccatcct
     2941 gcattatttg attaacaagt tttgtaacaa ttttgtcatt ataaatcggg tcaggtaata
     3001 tttcccgctt tggtacatgt ccttttcttg gcactatact tccctccttc cagtataata
     3061 taattattca tcggtactcg tggtcaacaa ccatcgtaag tgcctgccat gcttttacac
     3121 tacttcttgg ctgcaccggc ctttggcctt tttgcgccat acttggaacg actctgcttt
     3181 ctatttgcaa ctcccgctga atccagtgtt cctcttataa tatggtatct tacaccagga
     3241 aggtctttta ctcttcctcc tcttataagt acaacactat gctcctgcag gttgtgacca
     3301 attccaggta tatatgcagt aacttctata ccgttggtta atcttaccct tgcaactttc
     3361 ctcaatgctg agttaggctt tttaggtgta gtagttttta caacagtaca aacacctctt
     3421 ttttgaggac tgttaatttc agtaggtttc ttttttaaag aattaaagcc tctctgcaaa
     3481 gcaggtgagt tggatttttc taaaatatct tttcttcctt gtttaaccaa ctgattaaat
     3541 gtaggcattc acacacctcc tttcaccaaa attattttaa aatacataca actgaagcac
     3601 caacatctat attacatgct tttcccagtt ttttcatatc atctgcataa gttatttcta
     3661 tagatttctt ctcacacggc tccttaatac ttctaataac cttttcatca gcgtctcttg
     3721 caattataca cattttaacc tgatcgccct gtatagcttt taaagattgc ttagacccca
     3781 cagttttttt gctgttttta agaagctcca gcacattaag ctccattcct gcaccctccc
     3841 gttataacgg ctgcagcaga aacttgactg cttacctgcc attttacaca ctgcactatt
     3901 ttacatctca ataatgtgtt tgtcaataaa aaaattaaat accgggtgca tttaattcct
     3961 ttgaagaaat atattctaga agctatcgtt acagtactta gagccttctc taatgcttct
     4021 tacaataatt tcttcaaaaa taaaatatgc acctaaaatg ggtttttaag ctcgaaggct
     4081 atttgcttcc tgtagtaata gttacatccc tatatttttt catacctgta ccagcaggaa
     4141 taagtttacc aataattaca ttttccttaa ggcctacaag aggatcaact ttccctttta
     4201 ttgctgcatc tgttaatact ctggtggttt cctggaaaga agctgccgag aggaatgatt
     4261 cagttgccag cgaagctttg gttataccaa gcagtgcatg ttttccctct gcagtcttaa
     4321 gtccatcttt taaagcagtt tcattttctc tttcatattc aaacatatca acgaggcttc
     4381 caggaagcag gttggtatct ccggcatctt ctactttaat ttttcttaac atctgcctaa
     4441 ttattatttc aatatgcttg tcatttatat ctacaccctg cagtctgtaa actttttgca
     4501 cctcatgaag caggtatgat tgtacaccta ttaatccttt tattttcaat atgtcgtgag
     4561 ggtttacaga accttcagta agttcatctc cagcttctac ataatcccct tccataacct
     4621 ttatccttga accatatgga atcatataac tcttggattc tccaccatca ttgcttacta
     4681 ttacttctct ctttttcttt gtttcactta ttttaaccaa accggcaatt tccgatataa
     4741 cagccaatcc ttttggcttt ctagcttcaa acagctcctc tacacgtggt agaccctgtg
     4801 taatatcttc acctgcaaca ccacccgtat ggaatgttct catagtcaac tgggtaccgg
     4861 gttctcctat tgattgtgct gctataattc caacagattc tcctatgtcc acttctccgc
     4921 ctgtagcaag gtttgctcca taacattttg cacatactcc atatgcagaa tggcatgtta
     4981 ggattgatct gatttttact ttcttaatac cggcattttc aaccttttca gcttcgatat
     5041 cagttatcat ttttcctgct tctattatga cctgcccatt ttgagggtta attacatttt
     5101 ccagagcata tctgcccgta attctttcag ctaaatgctc aataacctgg cctccctcag
     5161 taatgtctga aacctcaaaa ccatgtcctg ttccgcaatc aaattctctt attataacat
     5221 cctggctgac atcaactaat cttctggtaa gataaccaga gtctgctgtt cttagagcag
     5281 tatctgccaa tccttttctg gtaccatgag ttgaaataaa atattcaagc acattaagtc
     5341 cttcacggaa attggacttg attggaattt ctatggtctt accctgtgta tcggccatta
     5401 agcctctcat tccagccaat tgttttatct ggttaatatt acctcttgca cctgatgtag
     5461 acatcatata aagaggatta aacttatcca gattatccat aagagcttta gtaatatttt
     5521 caccactttc ggtccaggct gaaataactg cattgtatct ttcattttct gatattagac
     5581 ctctcttata ttgtttagtt atattttcaa ttttttgttc tgtttcagct acataacgtt
     5641 tctttatttc gggaataatc atatctgtaa taccaatggt tatagcaccc ctggtggaat
     5701 acttgaatcc caatgctttt attttatcca gtactatagc tgtctttgtt gttccatgta
     5761 ccctaatgca tctgtaaatt attttttgca gagcttttct atctacaaga aaacctattt
     5821 cgaggctgaa taaattttca ggaacactcc tgtcaataaa tcctaagtct tgagggatag
     5881 cttcgttaaa taatagttta cccactgttg tgctaattat ttttgaaaca ggcttaccgt
     5941 caaattgctt ggtgattttt acctttattg gagcatgcaa agtcaacctg tcctcgttat
     6001 caatactagt ttcatatatc aaatcctgct gggactggtt ataagccatg attgcctctt
     6061 ctggagatga aaaagctctg attttgccct tataatcctg gttttctaca gtcaggtagt
     6121 atgttcctag aaccatgtcc tgtgttggta ccgttaccgg tttgccatcc tgaggcttta
     6181 aaaggttatt tgcggaaagc atcatgaatc ttgcttcagc ctgtgcctca acagataaag
     6241 gtacatgtac tgccatctgg tctccatcaa agtcggcgtt atatgctgta catactaaag
     6301 gatgcagttt tatagctctt ccttctacaa gtattggttc aaaggcttga ataccaagac
     6361 gatgcaaggt aggtgcacgg ttcaaaagta ccgggtgctc tttaattaca tcctccagaa
     6421 catcccatac ctcatccctg actctttcaa ccatacgttt tgcactcttt atattatgag
     6481 ccaatccgtc atttacaagc tttttcatta caaacggttt aaacagttca agagccattt
     6541 ctttaggcaa gccgcactga tagagtttaa gttccggacc tacaacaata acggaacgtc
     6601 cggagtagtc tacacgctta cccaaaaggt tctgacggaa acgtccctgc tttcctttca
     6661 gcatatcaga aagagatttt aaaggcctat ttccagggcc ggttacaggt cttcctcttc
     6721 tgccgttatc tattaatgca tccacagctt cctgcagcat tctcttttca tttcttacaa
     6781 taatatcagg agctcctaag tcaagcaacc tttttaatct gttgtttcta tttataactc
     6841 ttctataaag gtcattcaaa tctgaagtag caaatcttcc accatccagc tgaaccatag
     6901 gccgcaattc cggcggtata acaggaataa catcaagtat catccattca ggcctattac
     6961 ctgacatgcg aaaagcttcc actacttcaa gtctttttat agttctcatt cttttctgac
     7021 cggatgtttc atcaaattca gctttgagct ctttagaaag ctcttcaagg tcaatttcgg
     7081 ctaataattc cttaatggct tcagctccca ttccggcctt aaatttgttc ccaaatttct
     7141 ctacactatc tctatattct ttttcagaca aaatctgctt tttaaataaa ggggtctgcc
     7201 ccgggtcaag aacaatatat gaagcgaaat atagcacttt ttccagtgaa cgcggtgaca
     7261 tgtcaagaat cagacccatt ctgctgggta ttcccttaaa ataccaaata tgtgaaacag
     7321 gtgccgctaa ttcaatatgt cccattcttt cacgtcttac tttctgtttt gtaacttcaa
     7381 caccgcatct atcacaaaca atccctttat atcttattct tttatattta ccgcaatggc
     7441 attcccaatc cttcgtaggg ccgaatattc tttcacaaaa gagtccatcc ctctcaggtt
     7501 ttaatgttcg ataatttata gtttcaggtt ttttgacttc accttttgac cactccctta
     7561 ttttttccgg tgaggccagg ccaattctta tagcatcaaa agttccgagt tcaagcatgg
     7621 ctttttactc ctttcaaaat catcagatat tatcatcatc gtcatccaac tcatcattaa
     7681 cttcatcaag atcacctaaa tccacaagat catccaggtc ttcaaaactg actgattcag
     7741 ggtctatatc atcattttca aaatcatcgg tctcaagaaa taaattgctt attatatcgt
     7801 catcgtcatc agtattcccg gaagattctg gagattccag atcaaagtcg tccaattcaa
     7861 gttcgttgtc cagtacatca tcaccctcat cataatcgga taaaggttcc tcatcttctc
     7921 tgccttccat atttacattc agatcatcaa tatcatcatc aattgattct ttaattgata
     7981 cttcttcatt ctcttcagaa taaaccttca catcaagagc aagactttga agttctttta
     8041 tcaatacttt gaaggattca ggtatacctg gttcgggtac attttcacct ttaacaatgg
     8101 cttcatatgt tttaactctt ccgaccacat catcagactt aacagtaagg atttcctgca
     8161 atgtataggc ggcaccatat gcctcaagcg cccatacttc catttcccca aacctttgtc
     8221 ctccaaactg cgccttacct ccaagaggtt gctgtgtaac aagcgagtat ggccctgttg
     8281 aacgggcatg aattttatca tcaacaagat gggcaagctt caatatatac atatacccta
     8341 ctgtaacacg gttgtcaaaa ggttctccgg tcctgccgtc atacaatatg gttttgccat
     8401 cttcgttcag gtttgccgtt ttaagggttt ccataatgtc atgttcattt gctccatcaa
     8461 aaacaggagt t
//
LOCUS       DAOSDD010000199         8289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3206_length_8289_cov_3.884139, whole genome shotgun sequence.
ACCESSION   DAOSDD010000199 DAOSDD010000000
VERSION     DAOSDD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_8289_cov_3.884139"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK733_14920"
     CDS             <1..419
                     /locus_tag="PK733_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11867.1"
                     /translation="NLILPLISSLMTSDKNFANTFFSEIGNPTYMSRLGSEYNCKEWL
                     KLVIASLKGFSDSMGFDLYESEDGEKWEAISLNGLGNICNYGLRTFLVASDGRLYLGT
                     ANPFQGCEVWVKEDNNDEEEDNDWYWWLKDKCDFQL"
     gene            complement(615..3014)
                     /locus_tag="PK733_14925"
     CDS             complement(615..3014)
                     /locus_tag="PK733_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11868.1"
                     /translation="MKTRCFIVRKHKCVIRPVFIMAILLAAVCIPLHIHANDTSLGRF
                     GETVRPIDNPNIIMQSEDITVRISEGTSRVECEFVFINDSHEETTVLMGFPSGDSEDK
                     NGKQADWFYDYKLYDFAAWVDDEEIEVRLERGVGAEETGPDSLNFPYWHTWEVNFKPG
                     EKKIVKNTYETKNTVSSIGIIQAGYVLTTGAPWKGRIENAKIAFITEGIEPYQVESVT
                     PAGYAYEGNVIEWKFEDFEPDVNITVVFNVREKYLLKEQFKYDEKLNPIVEMEEQSKY
                     AEMVEFIDEIMRKKEYDTDSIYTESSLKLAKAKALLKIGNERAAKEAMGMLDNIADAG
                     GLGATEAAHILLGCYKDSGTDKHRELYDKKVFLRTNGVFQKLAVDMFPDLKSGYSPEI
                     TNLKVTAHKVCVNIMDKDDDLKKFSVEVWYMEDGKRLHLLDHQASDPMYFIYNHYTAR
                     YYGFLPGVAGNKQLYYRVCVEDWAGNAVDTGEREINAHAGDSGDDVYDGHEEHDEHKG
                     LDEQHVVAAVHWASKYAEMFSDMEEVPENITDGDKYILNKDLFNMLVKYSNLYPAEKY
                     RQRDIFSQFLSEDRFVTRAEALNVIICFLQRVQDFKVNMGILQASDEGKYKEKFGDWN
                     NVPPEFKDSMLVALKTGAVIGYLNNTLRPSGYITTNEALAILARIKYGEISRREDEGR
                     NKRNAYADIALKDEIASKWYYEHSGDKFVIHEDGQWYILEDSRGHAFDGIDGKIYKQK
                     CSEELAVETKNSMPEIYCEEKDGMVEINILQKLGSPPHRYVLIVNTNTLETIGREIRD
                     Y"
     gene            3656..4027
                     /locus_tag="PK733_14930"
     CDS             3656..4027
                     /locus_tag="PK733_14930"
                     /inference="COORDINATES: protein motif:HMM:NF025677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HOJ11869.1"
                     /translation="MNGNILKQIFFDENKNWDRFVNIHRKNIRKIVLEEVEKFRLCGE
                     KEAGLSLFACEACGDIKIVPHRCKGRFCSVCATGYMQEWSMKTAENMYDMPHRHIMCL
                     HRLKSCGKYFLLKLQTSLLNC"
     gene            3955..4389
                     /locus_tag="PK733_14935"
     CDS             3955..4389
                     /locus_tag="PK733_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOJ11870.1"
                     /translation="MFTSPEELWEIFFIEVTDKLAELLILFGRKDHADYKHCRVYRAY
                     YSLDANDGIEMDFTLLVMSPEEIYERKLSRQELYAAINSLLEKQAKRIYAHFFLNMSK
                     TEIARIEGVERSSVRNSIARGLKQMEKYLKNLIFLFTPPRFA"
     gene            complement(4420..5286)
                     /locus_tag="PK733_14940"
     CDS             complement(4420..5286)
                     /locus_tag="PK733_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOJ11871.1"
                     /translation="MFDTEGLHEKLIIFTEHRDTLRYLADKIRSLLGNEEAVVTIHGG
                     MLRDERRKVEELFKQDKDVRILIATDAAGEGINLQRAHLMVNYDLPWNPNRLEQRFGR
                     IHRIGQTEVCHLWNLVSKETREGMVFQRLFEKLEQEREALKGKVFDVLGKVTFNNRSL
                     RDLLIEAVRYGNDPAKKAQLYEVVDHSLDRKALQKLLDEHALTENTMDIHKVMTIRED
                     MERMEAHRLQPHFIETFFIEAFTSLGGKIRPREKGRMVFAYLQSRYPSSGHVGNVPDK
                     TIILFSKINLWG"
     gene            complement(5594..6091)
                     /locus_tag="PK733_14945"
     CDS             complement(5594..6091)
                     /locus_tag="PK733_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11872.1"
                     /translation="MSIICANMPSLILLTEYGGNYFEYENAVYELFIRDYIDTKPIFN
                     GQALALKRHPLFKNREATFWHIVSEGPTESERTPDLRRYERVRWPKSIIEFCHTSCAK
                     IKVWENNRKGEKRILLWCEDIEYLVVLADRKNYVLFWTAYPVTEGHRKRKLQKEYEDY
                     LNKSS"
     gene            complement(6088..6798)
                     /locus_tag="PK733_14950"
     CDS             complement(6088..6798)
                     /locus_tag="PK733_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HOJ11873.1"
                     /translation="MDQIFNIYCDESCHLLNDGEDIMVLGGISCQTEHVKNINQHIRR
                     IKSKHQVSPSMEIKWTKVSQGKLEFYKELIEYFFENPYLNFRSVIASGKQSLNYEAFN
                     LTHDNWYYRMYFLLLRQMIQIGDKYRIYIDIKDTRGSEKIHMLQEVLNRSLYHFCNET
                     VENIQLVRSHEIEILQLTDLLIGAISYANRTLKTSVAKLELIEIIKMKSGCNLIYSTS
                     PYEPKFNLFVWKPRGAQR"
     gene            complement(7176..7385)
                     /locus_tag="PK733_14955"
     CDS             complement(7176..7385)
                     /locus_tag="PK733_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11874.1"
                     /translation="MSSLPFRIQFFIEDQNSDCKKQLTVAISGNQRLKRLFLIGYVLE
                     GPAWTNKYEKRELQIIIVRFPKAFR"
     gene            complement(7555..7860)
                     /locus_tag="PK733_14960"
     CDS             complement(7555..7860)
                     /locus_tag="PK733_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11875.1"
                     /translation="MAAKTRKWEDIYNNLDALTNEDRDEIELKVKIIEQILEARKEKG
                     PTQAELEAISGVRQTFIARLENNRMDPQLTTILKILHPLGMTLAVVPIQEQRADDLK"
     gene            8032..8154
                     /locus_tag="PK733_14965"
     CDS             8032..8154
                     /locus_tag="PK733_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11876.1"
                     /translation="MRGFDKKCKTIVTNDIKKIIEDMDEEEEKIWSCLKQVFQD"
BASE COUNT         2374 a         1677 c         1463 g         2775 t
ORIGIN      
        1 caaatctgat tcttccactt atttcttctc ttatgacttc tgacaaaaat ttcgcgaata
       61 cctttttttc tgaaattggt aatccaactt atatgagtag attgggaagc gagtataatt
      121 gcaaggaatg gctaaaattg gtgattgctt cactaaaagg cttttcagat tcaatgggat
      181 ttgatcttta tgaatcagaa gacggagaaa aatgggaagc catatctctt aatggactag
      241 gaaatatttg taattatgga ttaagaacat ttcttgttgc atctgatgga agattatatc
      301 taggcactgc caatccattt caaggttgtg aagtatgggt taaagaggat aataatgatg
      361 aagaagaaga taatgattgg tattggtggc ttaaggataa atgtgatttc cagttataat
      421 ctatcggtaa gcaggtctta tatcttttta taagaccgct ttacatccct tatgaataaa
      481 gagtttcgag aaaatctgct ctcatcatcg tatagggttt tgtattatgt tataacttcc
      541 cgggtaacaa aaagataaca agaattttct taaagataac aagatgtccc cctgtctccc
      601 tgttccaccc gacactaata atctcttatt tctctcccga tggtttcaag ggtatttgtg
      661 ttaacaatca ggacataccg gtggggcggg ctgccgagtt tttgcagtat gtttatctcc
      721 accattccgt ccttttcttc gcaatatatc tcaggcatgg aattctttgt ttcaactgca
      781 agctcctccg aacacttctg tttgtatatt ttgccatcaa taccatcaaa cgcatgtcct
      841 ctgctatcct ccagtatata ccactgcccg tcctcgtgta tgacgaattt gtctcccgag
      901 tgctcgtagt accacttgga agctatctcg tcctttagtg caatatccgc atatgcattc
      961 cgcttattcc tgccttcatc ctctcttctt gatatttccc catactttat cctggccagg
     1021 atagccagtg cttcattggt ggtaatgtaa cctgatggcc tcaaggtgtt gttcaaatac
     1081 cctataaccg ctcctgtttt taaagcaacc agcatactgt ctttaaactc cggcggcaca
     1141 ttgttccaat caccaaactt ctccttgtat ttcccctcat ccgatgcctg cagtatcccc
     1201 atatttactt taaaatcctg tactctctgc aggaagcata ttatgacatt taaggcctct
     1261 gcccttgtca caaacctgtc ttcgcttaaa aattgcgaaa aaatatccct ctgtctgtat
     1321 ttttccgcag gatatagatt cgagtattta accagcatat taaacaggtc tttattcaat
     1381 atatatttat ctccatctgt tatattttcc ggtacctcct ccatatcaga aaacatttcc
     1441 gcatactttg aagcccaatg aacggctgcg acaacatgct gttcgtcaag ccctttatgc
     1501 tcatcatgct cttcatgtcc gtcatacacg tcatctccgg aatctccggc atgtgcattt
     1561 atttcccttt ccccggtatc taccgcattt ccggcccaat cctccacaca aacacggtaa
     1621 tacaactgct tattgccggc cactccaggg agaaatccgt aatacctcgc agtgtaatga
     1681 ttatatataa aatacattgg atcactagcc tggtggtcta agaggtgcag ccttttacca
     1741 tcttccatgt accagacttc caccgaaaat tttttaaggt cgtcatcctt gtccatgata
     1801 ttcacacaca ctttatgggc ggtcactttc aggtttgtta tttcagggga atacccgctt
     1861 ttcaagtccg ggaacatatc aaccgcaagt ttctggaaaa ccccgttggt tcttaagaac
     1921 actttcttat cataaagctc cctatgctta tctgttccgc tgtctttata gcagccgagc
     1981 aatatgtgcg ccgcttctgt ggctcccagt cctcccgcat ctgctatgtt gtcaagcatt
     2041 cccatggctt cctttgccgc gcgctcattt ccaatcttaa gaagggcttt tgcctttgcc
     2101 agtttcaagc tgctttcagt atatatgctg tcggtatcat attctttctt acgcatgatt
     2161 tcatcaataa actcaaccat ttccgcatac ttgctctgct cctccatttc aacaatgggg
     2221 ttcagttttt catcatattt aaactgctcc tttaacaggt atttttccct gacattgaac
     2281 actacagtta tatttacgtc aggttcaaaa tcctcaaact tccattcaat cacatttcct
     2341 tcatatgcat agccggcagg ggttacgctt tccacctgat atggctcaat gccttctgta
     2401 atgaaagcta tcttcgcgtt ttctatcctc cccttccagg gagcgccggt agtcaaaaca
     2461 tagcctgcct gtattattcc tatagaacta acagtatttt tggtctcata tgtattttta
     2521 acgattttct tttctccggg tttaaaatta acttcccatg tatgccagta tgggaaattc
     2581 aaactatccg gtcctgtttc ttcggctccc acgcctcttt caagccgcac ttcgatttct
     2641 tcatcatcaa cccatgcggc aaaatcgtaa agcttgtagt cataaaacca atctgcctgc
     2701 ttcccgtttt tgtcttccga atccccgctg gggaatccca taagcacggt tgtttcttca
     2761 tgggagtcat ttataaagac aaactcgcac tcaactctac tcgtcccctc cgaaatccga
     2821 acggttatat cctcggattg catgataatg ttcggattat caatagggcg tactgtttct
     2881 ccaaaccttc ccaggctcgt gtcattggca tggatatgta aaggtataca aacagcggcc
     2941 aataaaattg ccataataaa aaccgggcgg ataacacatt tgtgttttct cacgataaaa
     3001 cacctcgttt tcatattttt gccacatatt aacctgtcta aacacaatac cgacacaatg
     3061 caattggcat aatgtaattg tataattttt tacagaatgt ttccattaca ggataattaa
     3121 atttatctta ttaattcaga aaaccaagta aaaaccgttt ccacttcagg ttgggtagta
     3181 ttttcgaaag ctttaattca ttatgctttc gaaaaagttt cactgccata aatattgcaa
     3241 ctggaaatga ttcgccaatt agaaaatcgg cccttttggg gaagaaatgt tttgctctcc
     3301 gcttcgcttc cgtaatggcc tgcgaggagg gctggatggc cgaacatatg cttattttag
     3361 caatcaccaa tccgaaaggg gagcgcaaat atacttttct gcagtttcac taatagtttt
     3421 gttcatagta tcattgttat cttcaatttt ttctaagtta aaactcttgt tgttatatga
     3481 catacttttt actccatcca aattaatatt tttttaaaac gtataggaat ttatatgttt
     3541 tgagttcaat tgaagcagtt ggtgcaagta gattagaaga catacaccaa taatttgtat
     3601 ggtaataaat acctgttaaa attaatgatt aaatggtaaa atgtgaacta tgaatatgaa
     3661 tggaaatata cttaagcaaa tattctttga tgagaataaa aactgggata gatttgtaaa
     3721 tatccacagg aaaaatatca ggaaaatagt attggaagaa gtagaaaaat tcaggctatg
     3781 tggtgaaaaa gaagcaggcc ttagtctgtt tgcatgtgaa gcgtgtggag acataaagat
     3841 agtaccacac cgttgcaagg gcagattttg cagtgtatgt gccacagggt atatgcagga
     3901 atggagcatg aagacggctg aaaatatgta tgatatgccc catagacata ttatgtgttt
     3961 acatcgcctg aagagctgtg ggaaatattt tttattgaag ttacagacaa gcttgctgaa
     4021 ctgttaatac tatttggacg aaaagaccat gcagactata agcattgtcg tgtctacagg
     4081 gcttattact cactggacgc caatgatggt attgaaatgg actttactct tctggttatg
     4141 tctcctgaag aaatttatga gaggaagtta agtcgtcagg agctttatgc tgctataaac
     4201 agtttgctgg agaaacaggc aaaacgtatc tacgctcatt tttttctcaa catgagcaag
     4261 actgaaattg ccagaattga gggtgtagaa cgaagttccg tgaggaactc catcgcaaga
     4321 gggttgaagc aaatggagaa atatttaaaa aatttgattt ttttatttac cccaccccgt
     4381 tttgcctaaa aaatctgatg attagtagag ggatatttac tacccccaca aattgatttt
     4441 tgaaaataga ataattgtct tatccggtac attccccaca tgtcctgatg aaggatagcg
     4501 ggattgcagg tacgcgaaga ccattctccc tttttcacga ggacggattt tcccacccaa
     4561 gcttgtaaat gcttcaataa agaaggtttc tatgaagtgg ggctgtagcc tgtgtgcttc
     4621 catacgttcc atgtcttcgc ggattgtcat caccttatga atatccattg tattttctgt
     4681 caaggcgtgt tcgtctaata atttctggag tgctttacga tcaagggagt gatccaccac
     4741 ttcgtacagc tgtgcttttt tagcaggatc atttccgtag cgcactgctt cgatgagtag
     4801 gtcccgtaaa gagcggttgt taaatgtcac cttgccgaga acgtcgaaga ccttcccttt
     4861 aagagcttcc cgctcctgct ccaatttttc aaatagccgt tgaaacacca tgccctcacg
     4921 ggtttcttta gaaacaaggt tccataggtg gcagacttcg gtctgcccga tacgatgaat
     4981 acgtccaaaa cgctgttcaa gacgattggg gttccacggt agatcatagt tcaccatcaa
     5041 gtgtgctctt tgtagattga taccttctcc cgccgcatca gtggcaatga gaatgcgtac
     5101 atctttatcc tgtttgaaaa gctcttcgac tttacgccgt tcatctcgaa gcataccacc
     5161 atgaatggtc actacagctt cttcgtttcc taaaagtgaa cgtattttat cagccagata
     5221 ccgtagtgta tcccggtgtt cagtgaatat aataagcttt tcatgtaatc cttccgtatc
     5281 gaacatgtta ccgtcatttt gcagaagctt agagagttca tcccatttta tataagatat
     5341 acagtgcagc acgactttcc ttaaatattt ttgggtgtag aaagagttcg ttgatttttg
     5401 aatggtgcca ggaaggtagt atcaatttgc taaaatttga atataatata ttatacttgc
     5461 aagtaaattt gtatctcatt ttttcgaaat ttattgacaa taaagtcaat gtgagttaca
     5521 atagaaatag ctcatttacc atgtgtaaaa ggagtaacga tgccgtctaa gttaattagg
     5581 cggcatcagc cgttcatgaa gacttattta aataatcttc atattctttc tgcaatttgc
     5641 gttttctatg tccttcggtc actggatagg cagtccaaaa taaaacataa tttttcctgt
     5701 ctgctagtac cactaaatat tcaatatctt cacaccaaag taaaattctt ttttcacctt
     5761 tgcggttatt ttcccacact tttatttttg cgcaagatgt atgacagaat tcaataatgc
     5821 ttttaggcca gcgaacacgt tcatatcttc ttaagtctgg tgttctttca gattcagtag
     5881 gaccttctga tacaatgtgc cagaaggttg cttcacgatt tttaaaaaga ggatgtcgct
     5941 ttaaggctag agcttgccca ttgaaaatag gctttgtatc tatatagtct cttatgaata
     6001 attcatatac agcgttttcg tattcaaaat aattaccgcc atattctgta agcaggataa
     6061 gggatggcat gtttgcacat ataatactca tctttgagct ccacgaggtt tccatacaaa
     6121 aagattgaat ttaggttcat atggtgaagt tgaataaatt aagttacaac ctgatttcat
     6181 ttttataatt tcgatcagtt ccagtttggc gacacttgtt ttcagagttc tatttgcata
     6241 agagatcgct ccaataagta agtctgttag ttgaaggatt tctatctcat gagagcgaac
     6301 aagttgaata ttctcaacag tctcattaca gaaatgatat aggctcctat tcaatacctc
     6361 ttgaagcatg tgaatttttt ctgacccccg ggtatcttta atatcaatat atattctata
     6421 cttatctcca atttgtatca tctgtctaag taatagaaag tacattctat aataccaatt
     6481 atcgtgtgtt aagttaaatg cttcataatt aagactttgc ttaccggatg caattactga
     6541 tctgaaattc aaataaggat tttcaaagaa gtactctata agttctttat agaattcaag
     6601 cttaccctga gatacctttg tccactttat ttccattgaa ggactcactt gatgcttgct
     6661 tttaatccgg cgtatatgct ggttgatatt tttcacatgc tcagtttggc aggatattcc
     6721 tcccaagacc ataatgtctt caccatcatt tagtagatga caactttcat cacagtatat
     6781 attaaaaatt tgatccataa aatttacctc aactaaatat tgtacaagta tattctatca
     6841 tatataaagg agtagtgaag atataaaaaa cttaaaagta tcaggtagtt atcgtatata
     6901 gaaatagatt tgctgctctt aatccagatc atccatggat agacttgaag gttttagaat
     6961 tccttaagcg gcttcaggtg atatctaatg acagaaaaac ggaaaaaagt attttatatt
     7021 tgaatattgc ggccagggaa aaaagcaaag ttatcctcat attattataa tacaactaaa
     7081 ttaacctata tatttcaaca ttagctatgt ttatcaaaac acgtttgaag aactttgtta
     7141 caccacactg gatttactta ataatatcat tagttctatc taaaagcttt aggaaatcta
     7201 actataataa tctgcaattc tcttttttcg tatttattag tccatgctgg gccttcaagt
     7261 acataaccga ttagaaataa tctctttagc cgctgatttc cagaaatagc cactgtaagc
     7321 tgttttttgc agtcactatt ttgatcttca ataaaaaact gtatcctaaa tggtaatgaa
     7381 ctcaattgtc tgtgagatat ctccacattt tcattcgagg ttgaatggat acaattttct
     7441 cttgtaaagt cacttttaaa gaagtatttc ctgaagttgg atttttcttt cttaatccaa
     7501 attctttaca tgaaagcttc ttgccagcct tctataatgg aatattctct tatgttattt
     7561 aagatcatct gcacgctgct cttgaatagg aacaacggcc agtgtcatcc ccaatggatg
     7621 cagtatcttt aaaattgtag taagctgcgg atccattcgg ttattctcaa gcctagcgat
     7681 aaaggtttgc cttaccccgc ttatagcctc cagttcagct tgggttggcc ctttttcttt
     7741 gcgtgcttca agtatttgct ctatgatctt aaccttaagt tctatctcat cccggtcttc
     7801 atttgttaat gcatccaggt tattataaat gtcttcccat ttccttgtct tggcagccat
     7861 actatttgct cctttccaaa aaacctttta aattccgttt agcctgctct atttctcttt
     7921 gaggtgcttt tttgtccatt atttgtatac actaactaac ttgagttttg tacttttcta
     7981 tgagaatgct gaattcaaag ccacctgtta ttttacaaga aaaggcttaa aatgaggggt
     8041 tttgataaaa agtgcaaaac tatagtaact aatgatatta agaaaatcat tgaagacatg
     8101 gatgaggaag aagaaaaaat atggagttgt ttaaaacaag tttttcagga ttagtatata
     8161 catttttgat gattctgtat gcacctattt aactggattt aaatagggtt tgcccgaaat
     8221 aatttagtaa acacgctagg agtctgctcg gaaaagcgaa ctcgtgcaag aagaagtgtc
     8281 atttcctgc
//
LOCUS       DAOSDD010000200         8251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3217_length_8251_cov_5.171547, whole genome shotgun sequence.
ACCESSION   DAOSDD010000200 DAOSDD010000000
VERSION     DAOSDD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3217_length_8251_cov_5.171547"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="PK733_14970"
     CDS             complement(<1..1676)
                     /locus_tag="PK733_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOJ11877.1"
                     /translation="MGINNFEELKILADNINSCLEKQQKKVLVCAGTGCIAGGSLEVY
                     ERLKELAGQKDLLVNINLYHEEDHGNKCIGIKKSGCHGFCEAGPLVRIEPCNYLYLHV
                     KPEDCEEIVETTLIKGLPVERLMYTCENKVYPVQEEIPFYRKQTRIVLENCGHIDAES
                     IEEYIARGGYEALGKVLAEIKSEEVCALISESNLRGRGGGGYPAGRKWSQVLAEESDA
                     KYVVCNGDEGDPGAFMDRSIMEGDPHRVIEGMLIAGFATKSTEGYIYVRAEYPMAVKR
                     LEIAIDQARSHGLLGRNIMNSGFNFNIHVAQGAGAFVCGEGSALTASIEGERGMPRTK
                     PPRTVKEGLWRKPTVLNNVETFANVPHIILKGSEWFKSIGPAKSPGTKAFALTGSVNN
                     IGLIEVPMGTTLREIIYEIGGGIKDNKKFKAVQIGGPSGGCLTEKHLDLPLDFDSLES
                     VGAMIGSGGLVVMDEDTCMVEVARFFMNFTQNESCGKCIPCREGTKRMLEILERIVSG
                     DGTLDDLNLLESLGDTISKTSLCGLGKTAASPVISTLRQFKDEYIAHVVD"
     gene            complement(1789..2265)
                     /locus_tag="PK733_14975"
                     /pseudo
     CDS             complement(1789..2265)
                     /locus_tag="PK733_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
     gene            2622..4163
                     /gene="hflX"
                     /locus_tag="PK733_14980"
     CDS             2622..4163
                     /gene="hflX"
                     /locus_tag="PK733_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ11878.1"
                     /translation="MKDNMDQEHQNRNGKNAYQERVSGMIDGTIENANINTIDVKGNG
                     ASNERAILIGLETSSGYSGYSGYGEGRIIGGRTEEERSLDELEELAHTAGVTVVGKML
                     QKRPVRDSAHYIGSGKLEELGMFCNNMDAGTAIFDDELSGAQIRNIEEAIGVKVIDRT
                     TLILDIFAQRAHSREGKIQVELAQLKYRLPRLTGMGGQLSRLGGGIGTRGPGEKKLET
                     DRRHIRRRINYLETELKEVRKQRELIRENRRRNEFPTVALVGYTNAGKSTLLNKLCGA
                     DVFVEDKLFATLDPTTRKLAFSANVFGQTGQSNDDDNTLTDVERDILLIDTVGFIRKL
                     PTELIEAFKSTLEEAVYADALIHVVDATDEEYEEHIKVVNEILHSLGAENKPVILALN
                     KIDLFDANKVNEANEGNERIPERIPIKSAYGDLYSEVCEISAITGHGLDDLVKSIEGI
                     LSVNKVELNLMVPYNEGWVLPYIYENGRILEKDFADKGIKAKVVIENSKAYKIMKYAE
                     INTIS"
     gene            4309..4812
                     /locus_tag="PK733_14985"
     CDS             4309..4812
                     /locus_tag="PK733_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ11879.1"
                     /translation="MLVKNCAGALVFWDEKVFLLKSERGEWVFPKTAIRMDELPGEAA
                     LRAVGENAGIAAEIISTAGHASFEISSETHHKPIYNKIIWYVMISQDQEYLINEEKGF
                     TDGGYFSTKEAMGLLTHNHNKAMLNLLSRHILENASEYASENNLENASKHISQHVSEY
                     IPEPAMV"
     gene            4817..5455
                     /locus_tag="PK733_14990"
     CDS             4817..5455
                     /locus_tag="PK733_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOJ11880.1"
                     /translation="MLKQYKTILSHASAEIEEKKSRFIANVKPVTCEEEAVEFINEIK
                     SKYWDASHNVYAYYINGDNIIQRFSDDGEPSGTAGLPVLEVIKRMDLQDLVVVITRYF
                     GGTLLGAAGLVRAYGRSASSGVETAGIITKRLCVSISIIMGYPVFGKVQNQVINYGYN
                     IRNIEYTQDVEMVVLVPVDDVEMFIEFVNEVTNGEALIEIGEKEYVSWQSDG"
     gene            5430..5744
                     /locus_tag="PK733_14995"
     CDS             5430..5744
                     /locus_tag="PK733_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11881.1"
                     /translation="MYRGRVMVNMNEFITDLFSVDLLIQENSHIFVANIFSFYNTLFF
                     MVADAHKQGVLEPFPISDNIQRDIKIEELLRHVLAFLLLNMQGNILYFHKKDMVCSNL
                     FI"
     gene            6008..7153
                     /locus_tag="PK733_15000"
     CDS             6008..7153
                     /locus_tag="PK733_15000"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ11882.1"
                     /translation="MSRLEKIKDLIIYKDGHYNTFPNVIRRSDGTLMVGFRQATDRQK
                     KFIAGINKNFGKVTHIDSTSKAVYVTSKDEGATWEQKVNIIYDDFVYGVQDPCLNQLA
                     DGTIFTTFFMWKIFKKKDIKEILPSDRIIDDKYIARMDDAYSIRSFDGGNTWDEPVPI
                     QYPGKHYICVRGNIAVLDDGAIVLPVYGDNIAGETSKVVLLKTYDKGRSWEKLSVIPQ
                     VEGYGYYEPNIFKTESGKLVAFIRTHKIGGKSVYEPDSVKMTPLITCESFDDGKTWTQ
                     PATREIYSPSPFHPLRLKSGNVILTYGYRYKLYGLRAILLNAECNNFDEAEEVILRDD
                     GLGTDIGYTCSVLLNNGDVLITYYYFDEENGYRYIAGTICREVPSSI"
     gene            7185..7322
                     /locus_tag="PK733_15005"
                     /pseudo
     CDS             7185..7322
                     /locus_tag="PK733_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
     gene            7385..8131
                     /locus_tag="PK733_15010"
     CDS             7385..8131
                     /locus_tag="PK733_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOJ11883.1"
                     /translation="MLKELKKAVYEANLELVKKELVIYTWGNASGICREKGLVVIKPS
                     GVDYDALTPEDLVVLDLDGNIIEGKLKQSSDTPTHLVLYKEFTQIGGIVHTHSPWATI
                     FAQAGLSIPPLGTTHADYFYGEVPCTRKMFEKEINGDYEKETGNVIVETYKDRNINSD
                     FVPGVLVNEHGPFCWGKNPEESVYHAVVMEEIAMMAFNTLSLRHMAADGKTGGKTSDD
                     SSGVFQPMSPALLNKHFSRKHGKNSYYGQR"
BASE COUNT         2727 a         1370 c         1672 g         2482 t
ORIGIN      
        1 ttatccacta catgggcaat gtattcatct ttaaactgcc tgagagtact tattacagga
       61 ctggcagctg ttttgccaag tccgcaaagt gatgttttac tgatagtatc acccaggctt
      121 tcaagaaggt taagatcatc taaggtacca tctcctgata ctattctttc caatatttca
      181 agcattcttt tagtgccttc tctacagggt atacacttgc cgcaggattc attctgtgta
      241 aaattcatga aaaaccttgc tacttcgacc atgcaggtat cttcatccat aacaaccaat
      301 ccgccggaac ctatcatggc accaacactt tccaatgagt caaaatcaag gggaaggtca
      361 aggtgctttt ctgtaaggca tcctcctgag ggacccccaa tctggacagc tttaaatttt
      421 ttattgtctt ttatcccgcc gccaatttcg tatattattt ctcttagtgt agttcccata
      481 ggtacttcaa taaggccgat attatttacg cttccagtta atgcaaaggc tttagttccg
      541 ggacttttag cgggacctat tgatttaaac cattcactac cttttaatat gatgtggggt
      601 acattggcga aagtttctac attattcagc actgtaggtt ttctccataa cccctctttt
      661 actgtccttg ggggttttgt cctgggcatg cctctttcac cttcaataga agctgtaagg
      721 gcacttcctt caccacatac aaaagcaccg gcaccttgag ccacatgtat gttgaaatta
      781 aaacctgagt tcataatgtt ccgacctaac aatccatgag acctggcctg gtctatggct
      841 atttccagtc tttttacggc catgggatat tcagcacgga catatatgta tccttcagta
      901 cttttagttg caaatcctgc tattaacata ccttcaataa ctctatgggg atcaccctcc
      961 attatacttc tatccataaa agcccctggg tcgccttcat caccattgca gactacatat
     1021 ttggcgtcac tttcttcagc taaaacctgt gaccacttcc tgcctgccgg atagccaccg
     1081 cctccacgac ctcttaaatt tgattcactt atcaaggcgc agacttcttc agattttatt
     1141 tcagctaaaa cttttcctaa agcttcatag cctcctcttg caatatattc ttctatagat
     1201 tcagcatcaa tatgcccaca gttttccaga acaatacggg tttgttttct gtaaaaaggt
     1261 atttcctcct ggacggggta cactttattc tcgcaagtat acataagtct ttccacaggc
     1321 agtcctttta taagagtagt ttcaacaatc tcttcacagt cttcaggttt tacatgcaaa
     1381 taaaggtagt tgcatggttc aatccttaca agaggacctg cttcacaaaa accgtgacac
     1441 ccgctttttt tgattccaat acacttgttg ccgtgatctt cttcatgata aagatttata
     1501 ttgaccagaa ggtctttttg tccggctaat tctttgagcc tttcatatac ttcaagagat
     1561 ccgcctgcta tacatcctgt acctgcacaa actagaactt tcttttgctg cttttccaga
     1621 catgagttta tattgtccgc aagtatcttt aactcttcaa agttattaat tcccatagta
     1681 catctcctcc ttcatagcag tattcttcag ctcagtatcc ttcttaagat tcttcagagt
     1741 accagtctca tctggtgcag catcaacagc attaagggaa ttattttcat cattttttat
     1801 attcctaata agttttacag ccgaatccgg actaattttc ccgtatactt tgccattgat
     1861 ggtaataacc ggcgccagcc cgcacgcccc aagacaagat actgtctcta cagtaaacat
     1921 gagatcatcg gttgtatgtt tccggtcatt tagatgcaat tctttccgta aagcttcaag
     1981 aacaggtatg gactttttta catggcaggc agtcccgtca cagattttta taatatattt
     2041 gccttttggg ctcagtgaaa aattttcata gaatgtagct acactgaaaa cctttgaagg
     2101 actaatgttc attgaatctg atacatattc cagagcatct tcagacaaat agttaaattc
     2161 attctgaata tcatgaagaa cagatattaa cgatgattgc ctgcaaccat gtttattaat
     2221 aatttcatct attttagttg tccttgcaga agcaaacctt ttcataatta tatgtccctc
     2281 caaaaaagaa tatttaattt gtacttaata tgtagcattt actacaatat gccatacgaa
     2341 agcaatagca cgttgcacat tgtatattgt atacactaaa atgcacattg ttatttattt
     2401 aacaaatccg tatatataat ataatgctaa aagaatatta tcaagtaatt aataaaagga
     2461 ctttttttct taatttcggg gacatattat tatatacggt aaaataattt aatctgaata
     2521 atgtttatat cagaatcttt ttgtgttata ttattattgg gtcaattgac cccgggtgga
     2581 tgcatgtcaa aaaatataaa taaaggatga tttgtatagt agtgaaagat aatatggacc
     2641 aggaacatca aaacagaaac gggaaaaacg cttatcaaga acgagttagt gggatgattg
     2701 atggaaccat tgaaaatgct aatataaata ctattgatgt taagggtaat ggagccagca
     2761 atgaaagagc catattaata ggtcttgaaa ccagcagtgg atatagtgga tacagcggat
     2821 atggagaagg aagaataatc ggcggtagga cagaagagga aaggtcactg gacgaattag
     2881 aagagctggc acatactgcc ggggttactg ttgtaggtaa aatgctgcaa aaaagacctg
     2941 tcagagattc tgcccattat attggcagcg gtaaattaga agaattaggt atgttttgta
     3001 acaatatgga cgcaggtaca gctatttttg atgatgagct ttccggagct caaatccgaa
     3061 atattgaaga ggctataggt gttaaggtaa ttgacaggac cacactcatt cttgacatat
     3121 tcgcgcaaag ggctcattca agagaaggga aaatacaagt tgagcttgcc cagttaaagt
     3181 atagacttcc cagactaaca ggcatggggg gacagctgtc aagactcggg ggcggcattg
     3241 gtacaagagg gcctggagag aaaaaacttg aaacagacag aagacatatt agaaggagaa
     3301 taaactatct tgagactgaa cttaaggaag taaggaaaca aagggaactt atcagggaaa
     3361 atagaagaag aaatgaattt cccacagtag ccctggtagg ttatactaat gcagggaagt
     3421 caactcttct gaataaactc tgtggtgctg atgtttttgt agaagacaag ctatttgcta
     3481 ctcttgatcc aacaacaagg aaacttgcat ttagtgcgaa cgttttcgga caaaccggtc
     3541 aaagcaatga tgatgacaac actttgactg atgtggaaag agatattctc ctgattgaca
     3601 ctgtaggatt tatacgaaag ctgcctacag aattaataga ggcttttaaa tcaaccttgg
     3661 aggaagccgt ttatgctgat gcgttaattc atgttgttga cgcgactgac gaggaatatg
     3721 aggagcatat taaggttgtt aatgaaattc tgcatagcct tggagctgaa aacaagcctg
     3781 tgattcttgc gctaaataaa attgatttat ttgatgcaaa taaagtaaat gaggcaaatg
     3841 aagggaatga aagaatcccg gaaagaatcc cgataaaaag tgcttatgga gatttgtata
     3901 gtgaagtatg tgaaatctct gctataacag gccatggcct ggacgacctt gttaaaagta
     3961 ttgaaggaat tctttccgtg aataaagttg agttaaacct gatggtgcca tataacgagg
     4021 gatgggtttt gccttatatt tacgaaaacg ggaggatatt ggaaaaagac tttgccgata
     4081 aaggaattaa ggctaaagta gtaattgaaa attcgaaagc ttataaaata atgaagtatg
     4141 ctgaaataaa tactatttca taaagattac aattattctt gaaatgttga ttaaaaaccc
     4201 ttttttggtt attataataa tagaggatat ttttagtagg atatttaaag caggatagtt
     4261 ttattaaaac attatccaat aataaagagc cggaaagggg tttttgctat gcttgttaaa
     4321 aactgcgcag gtgctttagt cttttgggat gaaaaagtct tccttctcaa aagtgagaga
     4381 ggggagtggg tttttccaaa gactgcaata agaatggatg aactacctgg tgaagctgct
     4441 ttaagagcag ttggagaaaa tgccggaata gcggcagaaa taatatcgac tgcaggtcat
     4501 gcaagttttg aaatatcttc agaaacacat cataaaccaa tctataataa aattatctgg
     4561 tatgtaatga tatcacagga tcaagaatat ttaatcaacg aagaaaaagg gttcacggat
     4621 ggtggttact tctcaacaaa agaagctatg gggcttttaa cacacaatca taataaagca
     4681 atgcttaatt tattatccag gcatatctta gaaaatgctt cagagtatgc ttcagaaaat
     4741 aatttagaaa atgcctcaaa acatatttcc cagcatgttt cagagtacat tccagaacca
     4801 gccatggtgt gaggggatgc tgaaacaata caaaactatt ttaagccatg cttcagccga
     4861 aatagaggag aaaaaatcaa ggtttatagc taatgtaaaa ccagttacat gtgaagaaga
     4921 agcagttgaa tttattaatg agataaaatc aaaatactgg gatgccagtc ataatgttta
     4981 tgcatattat attaatggtg ataatattat tcagaggttc agtgatgatg gggagccttc
     5041 tgggactgcg ggacttcctg tgcttgaagt gataaagaga atggatcttc aggacctggt
     5101 agtggttata acaagatatt ttggcggcac tttgctgggg gcagcaggac ttgtgagggc
     5161 atatggcagg agcgcatctt cgggtgttga aactgccgga ataataacaa aaagattgtg
     5221 tgtgtcaatt agtataataa tgggatatcc tgtattcggc aaggtacaga atcaggtcat
     5281 taattatggc tataatataa gaaatattga atatactcaa gatgtggaaa tggtagtatt
     5341 ggttccggtt gatgatgttg aaatgtttat agaatttgtt aatgaagtaa ctaatggaga
     5401 agcactgatc gagattgggg aaaaagagta tgtatcgtgg cagagtgatg gttaatatga
     5461 atgaatttat aacagatttg ttttctgttg atttacttat tcaggaaaat tcacatattt
     5521 ttgttgccaa tatattcagt ttttataaca cattgttttt tatggtggcc gatgcccata
     5581 agcagggtgt ccttgaaccc ttccccatat ctgataatat tcaaagagac ataaaaatag
     5641 aagagctttt aaggcatgtt ttggcgttcc tccttctgaa tatgcaagga aatatattgt
     5701 acttccataa gaaagatatg gtatgttcca atttgtttat ataatacgtt gcgatatgaa
     5761 ttattcatga taatttcgtg aaggaacata tatccaatta taaaaatctt gtgaatgagt
     5821 tttggcagag tgtaggatta taaaaattct gtgccaacga aagcccgaac ggggggtgct
     5881 tgacagcaaa atttcctttg atagtataaa aatatcctgt taggaaacat aaaactgaac
     5941 tatcatattc aagttacaat atgatagtta taagaatgtt atagggataa aggaggaatt
     6001 agttttgatg agcagattgg aaaaaattaa agatttgatc atatacaagg atgggcatta
     6061 caacactttc ccaaatgtaa taaggagaag tgacgggact cttatggtgg gtttcaggca
     6121 agctaccgac cgtcaaaaaa aatttattgc cgggattaat aaaaattttg gcaaggttac
     6181 ccatatagac tctacttcaa aagcagtata tgtaacatca aaagatgaag gtgccacatg
     6241 ggagcaaaaa gtaaatatta tatatgatga ttttgtatat ggagtgcaag atccctgcct
     6301 gaaccaatta gcagacggaa caatattcac cacatttttc atgtggaaaa tattcaaaaa
     6361 gaaagatatt aaagaaattc taccttctga tagaattatt gatgataaat atattgcaag
     6421 aatggatgat gcttattcaa taaggtcatt tgatggagga aacacatggg atgaaccagt
     6481 accaatacaa tatcccggga aacattatat atgtgtaagg ggtaatattg cggttctgga
     6541 tgatggtgcc atagtattac ctgtatatgg agataatata gcaggtgaaa ccagcaaagt
     6601 tgtactttta aagacatatg ataaaggcag aagctgggag aaactttcag tcatcccgca
     6661 agtggaaggc tatggctatt atgaaccgaa catatttaag actgaatcag gaaaacttgt
     6721 tgcttttatc agaactcaca aaatcggggg taaaagtgtt tatgagcctg atagtgtaaa
     6781 aatgactccg ttaataacct gtgagtcttt tgatgacggt aaaacatgga cccaacctgc
     6841 cacaagggaa atttattcgc ccagtccttt tcatccatta agactaaaaa gcggtaatgt
     6901 tattttaacc tatggatata gatataagct ttatggctta agagcaattt tactaaacgc
     6961 tgaatgcaat aattttgatg aagccgagga agttatttta agggatgacg gacttgggac
     7021 tgatataggt tacacatgtt cagtattgtt aaataatgga gatgtgctga ttacatatta
     7081 ttactttgat gaagaaaacg gatacagata tattgcgggt actatttgca gagaagtacc
     7141 gagctctatt tagaagggta aaccctcttc catataagga tgtatacatt attgttgatg
     7201 gaatcaaagg taaaggtcaa tacatagaga cttatatggc atggggtgtt gataacaatg
     7261 gatggtgggg agaaggtgaa attaagttct acatagatgg agatgatgag ttccctacaa
     7321 tagaagaata tataattaat agtacatgca actatgtttg attatattaa gagcggtgat
     7381 aaaaatgctt aaagaattga aaaaagctgt atatgaggca aatcttgaac ttgtgaaaaa
     7441 agagcttgtt atttatacat ggggtaatgc cagcggcata tgcagagaaa aaggacttgt
     7501 agtaataaag ccaagcggag ttgactatga tgctcttacc cctgaagatc ttgtggtgct
     7561 tgaccttgat ggcaatatta tagaaggtaa acttaaacaa tcatcagata cacctaccca
     7621 tttagtatta tataaagaat ttacccaaat tggagggatt gtacatactc attcgccatg
     7681 ggcaacaata tttgctcagg caggcttatc cataccacct cttggaacta cacatgcaga
     7741 ctacttctat ggggaagtac catgtaccag aaaaatgttt gaaaaagaaa tcaatggaga
     7801 ttatgaaaaa gaaaccggta atgttattgt agaaacttac aaggatagaa atattaattc
     7861 tgattttgtt cctggggtcc tggttaacga gcatggtcca ttctgttggg gaaagaatcc
     7921 cgaagaatca gtttaccatg cggtggttat ggaagaaatt gcaatgatgg catttaatac
     7981 tttatccctg cggcatatgg cagcggacgg gaaaacaggt gggaaaacat ctgatgattc
     8041 ttcaggagtt ttccaaccaa tgtcaccggc acttcttaat aagcattttt cgagaaaaca
     8101 cgggaagaat tcctactatg gacagagata ggaagaaagc ttcatcaaag cttatataaa
     8161 caactcccag gtttcttttg ttaagaaaag aaagccataa accataaatg tatagccatt
     8221 tagttatata taaatataaa aagttatatt a
//
LOCUS       DAOSDD010000201         8247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3220_length_8247_cov_3.903198, whole genome shotgun sequence.
ACCESSION   DAOSDD010000201 DAOSDD010000000
VERSION     DAOSDD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3220_length_8247_cov_3.903198"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..711)
                     /gene="spoVT"
                     /locus_tag="PK733_15015"
     CDS             complement(157..711)
                     /gene="spoVT"
                     /locus_tag="PK733_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOJ11884.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIHEGDPLEIFTDREGEI
                     ILKKYSPIGELGEFATQYAESLHKTSGHIACITDKDSIIAVSGASKKDFVDKSLSKDL
                     EKVITDKSTVIVKSPEEQTIKITADENGERQYTSQIVKPIISEGDPIGAVIFLSTDPD
                     AYMGVVEEKLVESAAGFLGKQIEQ"
     gene            complement(859..2004)
                     /locus_tag="PK733_15020"
     CDS             complement(859..2004)
                     /locus_tag="PK733_15020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ11885.1"
                     /translation="MSKGMVKKSEETGNKTKAGNSSKYTLFAVVGIAVIIVTVAAVAG
                     FSAFSKSYVASVGKVKIKIDEYRFFLEQEKSNMLNIAGNPDPGTFWDTTITGGEKAID
                     IAKKKALENIRELKIQLMKAKEQKISLDKAEIDNIEKGIEEIITQYNSKSAADAACRE
                     INGVGLDEYKEIYKDYLLMNKLVQKEMESIEANEDEIEEYYNKFPDTFKDTIYRPNGQ
                     EAVWVRHILIATVDLQTEEKKELPGDKLEEVKKKAEELFERAKNGEDFAKLVAENSED
                     SGSAQRGGDYIFSEGGYMMPEFEETAFKLEPGNIDITKTDYGYHIIKLEEKFSQGEPV
                     SLRCAKEYWEFKSNAVKVAKYIEKLEEWKKDPKYRVVKNESVYNSIK"
     gene            complement(2064..5597)
                     /gene="mfd"
                     /locus_tag="PK733_15025"
     CDS             complement(2064..5597)
                     /gene="mfd"
                     /locus_tag="PK733_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ11886.1"
                     /translation="MNYLIEPLFELKEYNKILESIEDSTNPVSIVGPSDSQKAHFAYA
                     VCRHLNRRGLFITFNEIQARRMYEDLSFFLGEQVLLYPTKEIIYYNVEAKSNDEIFRR
                     ITILDKMLEEKYQFIVASIEAISHKITPRQLFCENILSFECGSREDLGKATEKLINIG
                     YERTDTVEGKGQFAARGGILDIFSVDREDPFRIEMFDDEIDSIRVFDALSQRSIDKLE
                     KVRVLPAREIICFGDKKEGIAGNIKEDLDKYIKNLKNKEDSDFVKHIKANIKRDIERF
                     EVSSYFPGADRYIPYILKEPGHISDYMEDNIIVFLDEASRINQRMDNLLLEHHEFCKG
                     LLEKGRILPGNIDIYHDYEDISRRLKKKQNVYISTVISGEYGGFDKTGKFESYNILSR
                     QMNSYHGFMDMLANDIAGWKQKNNRIVILAGIKARGEKLRDELISNGIEALYMEEINM
                     ELLPGKVIIARGSLNKGFEYPSIGLVVVSGREVFGFDRKARKVAKKTKGVKVSLFTDL
                     NPGDYVVHQTNGIGQYIGIEKLIVDNIKRDYLKIRYQQGDFLYIPTSQLDTIQKYIGA
                     EGKAPKLSKLGGSDWIKTKKKVKESLKELAEQLIKLYAQRRAVKGFVFSKDTVWQKQF
                     EDLFPYEETEDQLKCIEEIKVDMESDKPMDRLLCGDVGYGKTEVALRALFKSVMDGKQ
                     VAFLVPTTVLAQQHYNNFKERLKDFPVAVEMISRFRTRSEQNKILKDLKRGHIDILIG
                     THRLLQKDIQFSNLGLLIVDEEQRFGVVHKERIKNIRPDVDVLTLTATPIPRTLHMSL
                     IGVRDISVIEEPPEDRYPVQTYVMEYDNEIIKDSINRELGRGGQVFYLYNHVRTIDIK
                     GFQIQALVPDARVAIAHGQMEERRLEDVMLAFINREFDVLVCTTIIESGLDMPNVNTI
                     IVEDADKMGLAQLYQLRGRVGRSNRLAYAYITYKKDRVLTEIAEKRLQTIKEFTEFGS
                     GFKIAMRDLEIRGAGNLLGSEQHGHIESVGYEMYCRLLEEAITELKGEVPSHKDIEVT
                     IDLKVNAYIDDKYINTEIQKIEMYKKIASIQDEKDVIDIRDELLDRYGDLPDSVENLI
                     QIAHIKTLARDLNFISISEKGDSVIFHVKDEKSISIETIGKLTAKYKKQMLYNAGASP
                     YLMYRITGNNREKFLENIKIMLHDIKSFEVK"
     gene            complement(5691..6260)
                     /gene="pth"
                     /locus_tag="PK733_15030"
     CDS             complement(5691..6260)
                     /gene="pth"
                     /locus_tag="PK733_15030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ11887.1"
                     /translation="MEGYHILAGLGNPGLKYRNTRHNVGFDTIDILSKQYGINIRQLK
                     HKALIGDGFIEDKRVLLVKPQTYMNASGESIRDIVEWYKTPMESLILIYDDVDLPLGK
                     IRIRRGGSAGTHNGMKSVIYHLQRDCFPRVRIGIDKAPEHMDIIKYVLSKFTKDERDV
                     INHGVLKAAEAVVTVIKSGVDEAMTKFNG"
     gene            complement(6409..7362)
                     /locus_tag="PK733_15035"
     CDS             complement(6409..7362)
                     /locus_tag="PK733_15035"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOJ11888.1"
                     /translation="MNLHGKDIKIFSGNSHRTLAEEIAEKIGLPVGIATVGKFSDGEI
                     EVNIGEVVRGSDVFVIQSTCYPVNDNLMELLIMVDALKRASAGRITAVIPYFGYARQD
                     RKAKARDPISAKLVANLITVAGADRVLTMDLHVPQLQGFFDIPVDHLLGVPILAEYFK
                     EKFSGLDDLVIVSPDVGSVSRARKLAECLDAPIAIVDKRRPKANVSEVMTIIGDVSNK
                     RAILIDDLIDTAGTIVNSAEALAKLGAKEVYACGTHGVLSGPAVERIKKSPIKELVVL
                     STIPLTQEKSIDKIVSISVAPIFAEAIERIYGDISISSLFT"
     gene            complement(7444..8247)
                     /locus_tag="PK733_15040"
                     /pseudo
     CDS             complement(7444..8247)
                     /locus_tag="PK733_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512915.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
BASE COUNT         2237 a         1888 c         1128 g         2994 t
ORIGIN      
        1 ttttatgagt tcgattttgc ccggagctga atttaaaggc tctaagcggc gtaacgtcag
       61 ctcctggtaa atatttcttc acttttaaaa aatgcaccct ataataccat tcatcattac
      121 aacgttctgc acttattaat gatcaatctt atatacctat tgttctattt gtttccctaa
      181 aaatcctgca gcagattcaa caagtttctc ttcgaccact cccatgtatg catcaggatc
      241 tgttgaaaga aatattacgg cacctattgg atccccttct gaaataatag gttttactat
      301 ctgggatgta tactgtcttt ctccattttc atcagcagta atctttatag tttgttcttc
      361 cggagatttc acaataaccg tggatttgtc agtaattacc ttttcaagat ccttgcttaa
      421 agacttatct acgaagtctt tctttgaagc gcctgaaaca gcaattattg agtccttgtc
      481 agttatgcat gctatatgac cgctggtctt gtggagagac tctgcatatt gggtcgcaaa
      541 ctcacctaat tcacctatgg gtgaatactt ctttagtata atctctcctt ctctatctgt
      601 aaatatctcc agagggtctc cttcatggat cctcagtgtc cttcttattt ctttaggtat
      661 aacaactctt ccaaggtcat cgatccttct tactatacct gtagccttca atgcaactac
      721 ctcctccaaa aacttatttt tgattacgtt tttagtatta tttttttgtt ggagttttat
      781 acattttagg ttaattgtga aaatataaaa aggatatcaa tttcgtaaaa taaccattaa
      841 atacctgcca ttaaatattc attttataga gttataaaca gattcatttt ttacaacccg
      901 gtatttagga tcctttttcc attcttccaa cttttctata tactttgcaa cttttactgc
      961 atttgattta aactcccaat attcctttgc acatcttaag cttacaggtt ccccctgaga
     1021 aaatttttct tcaagtttaa ttatgtgata cccataatca gttttagtta tgtcgatatt
     1081 acctggttct agtttaaaag cagtctcctc aaattcaggc atcatataac caccctcact
     1141 gaatatataa tctccgccac gttgagccga acccgaatct tcagagtttt ctgcaacaag
     1201 cttagcaaag tcttctccgt tttttgccct ttcaaataat tcttccgcct tcttcttgac
     1261 ttcttcgagt ttgtcaccgg gtagctcttt cttttcctca gtttgcaaat ctactgtagc
     1321 tataagtata tgccgtaccc aaactgcctc ctggccattt ggtctataga ttgtgtcttt
     1381 gaaggtatca ggaaacttat tgtagtactc ttcaatttca tcttcatttg cttcaattga
     1441 ctccatttct ttttgcacca gtttattcat tagaagataa tctttataaa tctctttata
     1501 ctcatctaaa cctaccccgt taatttccct gcatgcagca tcagcagccg acttactatt
     1561 atactgtgta attatctctt ctatgccttt ttcgatatta tctatttcgg ctttatccag
     1621 ggatattttc tgttccttgg ctttcatcaa ctgaattttt agttccctta tattctcaag
     1681 ggctttcttt tttgcaatat ctatggcctt ttccccacca gtaatggtag tatcccagaa
     1741 tgttccaggg tcgggatttc ctgcaatatt cagcatattg cttttttcct gctccaggaa
     1801 aaacctgtat tcatcaattt ttattttcac ttttcctaca gaagccacat aactttttga
     1861 aaaggcagaa aaacctgcca ccgctgcaac tgtaactatt attacagcaa ttccgacaac
     1921 tgcaaataat gtgtatttac tactattacc tgcttttgtt ttatttcctg tttcttcgct
     1981 ttttttaacc attcccttac tcaaatatat ctctcctctt aataatcatg ataaccatgc
     2041 tcgttttatt atacatcaac tgctcacttt acttcaaagc tttttatatc atgtaacata
     2101 atcttaatat tttcaaggaa cttttctcta ttatttccag taattctata cataagatac
     2161 ggactggcac ctgcgttata caacatctgc tttttatatt tggcggtaag tttaccaata
     2221 gtttcaatac ttatgctttt ttcgtccttg acatgaaaaa ttacagaatc ccctttttca
     2281 gatatggata taaagttaag atcccttgcc agagttttta tatgggcgat ttgtataagg
     2341 ttttcaacag aatcgggtaa atctccataa cggtcaagta actcatcccg tatatctata
     2401 acatccttct catcttgtat ggatgcaatt tttttataca tttctatttt ctgaatttca
     2461 gtatttatat acttatcatc tatataagca ttaactttaa ggtcaatggt aacttcaata
     2521 tccttgtgag aaggcacttc tcccttcagc tctgttattg cttcctcaag caacctgcag
     2581 tacatttcat aacctacgga ttcaatatgg ccatgttgtt cagaacctaa tagattaccc
     2641 gctcccctga tttccaagtc tctcattgca atcttaaatc ctgaaccaaa ctctgtaaat
     2701 tcttttatag tctgaagtct cttttcagca atttcagtta gaactctatc ttttttataa
     2761 gttatataag catatgccag cctattcgac cgtcccaccc ttcccctaag ttggtataat
     2821 tgagctagtc ccattttgtc cgcatcctca acaattatgg tatttacatt aggcatatca
     2881 agtccggact caattatagt agtgcagaca agtacatcaa attccctatt tataaaagca
     2941 agcattacat cttccagcct tctttcttcc atttgcccat gggcaattgc taccctggca
     3001 tcaggaacca atgcctgtat ttgaaaacct ttaatatcaa tagtccttac atgattatac
     3061 aggtaaaata cctgcccgcc tctacccaat tccctgttaa tagaatcttt tattatttca
     3121 ttatcatatt ccattacata agtctgtaca ggatagcggt cttcaggagg ctcttctata
     3181 acacttatgt ccctaacgcc aataagagac atgtgaaggg ttcttggtat aggagtagct
     3241 gttagagtga ggacatcaac atcaggcctt atattcttaa ttctctcctt atgcacaact
     3301 ccgaagcgct gctcctcatc aactattaac aaacctagat tgctaaactg tatatctttt
     3361 tgcaacaacc tgtgagtacc tatcaatata tcaatatgtc ccctctttaa gtcttttagt
     3421 atcttgtttt gctctgatct tgtcctgaat ctgcttatca tttctacagc aacaggaaaa
     3481 tctttaagcc tttctttaaa attattataa tgctgctgag ctaatactgt agtaggtact
     3541 agaaatgcaa cctgtttacc gtccataaca gatttaaata gtgcccttaa agctacctca
     3601 gtctttccat atccaacatc accacataaa agcctatcca taggtttatc agattccata
     3661 tctactttaa tttcttctat acatttcaac tggtcttcag tctcttcata aggaaataaa
     3721 tcttcaaact gcttctgcca tactgtgtcc tttgaaaaaa caaaaccttt tactgctctc
     3781 ctttgtgcgt ataacttaat taactgttcc gctaattctt ttaatgattc ctttaccttt
     3841 ttctttgttt ttatccaatc gcttccgccc agtttactta atttgggggc tttcccttcg
     3901 gctcctatgt atttctgaat agtatccagc tgacttgtag gtatatagag aaaatctccc
     3961 tgctggtacc ttatcttgag ataatccctt ttaatattgt ctacaataag tttctctatt
     4021 ccaatatatt gacctatacc attagtttga tgtactacat aatctccagg attcaggtct
     4081 gtaaaaaggc tgacttttac tccttttgtc ttcttggcaa cttttctggc cttcctgtca
     4141 aaaccaaata cctctcttcc acttaccaca accaaaccta ttgaaggata ttcaaaacct
     4201 ttattaagac tgccacgggc aataatcact ttcccaggca ataattccat atttatttct
     4261 tccatgtata gtgcttcaat accattggaa atcagttcat cacggagttt ttcccctcgc
     4321 gccttgatac ctgccaatat tacaattctg ttgttttttt gtttccagcc agctatatca
     4381 tttgcaagca tatccataaa tccatggtat gagttcattt gcctggataa aatgttatag
     4441 ctctcaaatt tcccggtttt atcaaacccg ccatattccc ctgaaataac agtgcttata
     4501 taaacattct gctttttctt caatctcctg gatatatctt cataatcatg gtatatatca
     4561 atatttccag gtagaattct gcctttttcc agcaatcctt tacaaaactc atgatgttct
     4621 aaaagcaaat tatccatcct ttggttaata cggctggctt catccagaaa aacaataata
     4681 ttatcttcca tataatctga aatatggcct ggttccttca gaatatatgg tatatagcga
     4741 tctgctccag gaaagtagga gcttacttca aatcgttcaa tatcccgctt tatgtttgcc
     4801 ttaatgtgtt ttacaaaatc tgaatcttcc ttattcttca gattttttat atatttatcc
     4861 agatcctctt ttatatttcc ggctattcct tcttttttat caccgaagca aattatttct
     4921 cttgcaggta agactctaac tttttccaat ttatctatgg agcgctggga taaagcatca
     4981 aagactctga tagaatctat ttcgtcatca aacatttcta tcctgaaagg gtcttccctg
     5041 tccactgaaa aaatgtccag tatcccgcct ctcgcagcaa attgtccttt tccctctact
     5101 gtatcggtcc tttcatagcc aatattgata agtttttctg ttgctttccc caggtcctcc
     5161 cttgaaccgc attcaaaaga taatatgttc tcacaaaaaa gctgcctagg tgttatttta
     5221 tgggaaatgg cctcaataga tgcgacaatg aattgatact tttcctccaa cattttgtca
     5281 agtatcgtta ttctccggaa aatttcatca ttgcttttag cttctacatt ataatatata
     5341 atttcttttg ttgggtataa gagaacctgt tctccaagaa aaaatgaaag atcttcatac
     5401 atgcgtcttg cctgaatttc gttaaaagta atgaatagtc ccctgcggtt aagatgcctg
     5461 cagacagcat aagcaaaatg ggccttttga gaatcagaag gccccactat acttacagga
     5521 tttgtagaat cctcaataga ttccaatatt ttattatatt ctttaagttc aaaaagaggc
     5581 tcaattaaat aattcatatt tatatcttgg gcgtggagac tcttggctta aatcgtgaga
     5641 ggaaacgctc atcctcctta tcctttgtaa ttgaatatta tcattcttac ttaaccgtta
     5701 aatttagtca ttgcttcatc aacacctgat tttataacag taactacagc ttctgcggct
     5761 tttaaaacac cgtggttaat aacatctctt tcatctttag tgaacttgct cagcacatat
     5821 ttaataatat ccatatgctc cggagcttta tctatcccta ttcttaccct tggaaagcaa
     5881 tctcgctgca aatgataaat aaccgacttc attccgttgt gagttccggc acttccgccc
     5941 cgccgtattc taatcttccc aaggggtaaa tccacatcat catatattaa tataagactt
     6001 tccattggag ttttatacca ctcaactata tctcttatac tttccccact ggcattcata
     6061 tatgtctggg gttttacaag caaaactctt ttatcttcta tgaagccatc tccaataaga
     6121 gccttatgct taagctgcct tatatttata ccatattgtt tagacaatat atcgatagta
     6181 tcgaaaccaa cattatgcct ggtattccga tattttaatc ccggattacc cagtcctgcc
     6241 agtatatgat agccttccaa gcaatctgct ccttcccgat ttacactctc cttttattct
     6301 accgctccgg cttacattct ccttttattc taccgcataa acccaagttt gagtattact
     6361 accgcagcgc aggcttactc atttgcagct atacactgcg gtataacgct acgtaaacaa
     6421 ggaacttata gaaatatccc cataaattct ttcaatagct tctgcgaata taggagctac
     6481 ggaaatcgat actattttat ctatactttt ctcctgggtc aagggtattg tacttaacac
     6541 cacaagttct tttatcggcg acttttttat tctttcaact gcaggacccg atagcactcc
     6601 atgagtacca caagcatata cttcttttgc tcctaatttg gctaatgctt ccgcagaatt
     6661 aacaatggtc ccggcagtat caatcaagtc atctataagt atggccctct tatttgaaac
     6721 atcaccgatt atggtcataa cctcacttac gttcgcctta ggccttcttt tatctacaat
     6781 tgcaatagga gcatcaagac attcggcaag ttttctcgct ctgcttacgc ttcccacatc
     6841 tggagatact attaccaaat catccaatcc actgaacttc tctttaaagt actctgctaa
     6901 tattggcact cccagaagat gatctaccgg gatatcaaaa aagccctgca gttgtggtac
     6961 atgcagatcc atagtcaata ccctgtcggc tccggcaact gtaataaggt tagctaccag
     7021 tttggctgat atgggatccc ttgcctttgc ctttctgtcc tgccttgcat atccaaagta
     7081 cggtataact gccgtaattc taccagccga ggctctttta agagcatcaa ccattataag
     7141 aagttccatg aggttatcat ttaccggata gcatgtagat tggataacaa atacatctga
     7201 gccccttacc acttccccaa tattaacctc tatttctcca tcgctaaact ttcctacagt
     7261 ggcaattccc acaggaagcc ctattttttc agcaatttcc tctgctaatg tcctatgtga
     7321 gtttcctgaa aagattttta tatccttgcc atgtaaattc attttgcacc cttctttcat
     7381 tagttttctt caattttgtc agttttcatt gtttttgact ctactatacc attatttttt
     7441 cttctattct ttatcatttt tttctttttg ttgttctttt ctctccatat tctttttttt
     7501 tacccaatcc tcttttatta cctgcctttc tctcgcaatt gcaagagaat attccgggac
     7561 ctcatcagta attgttgaac ctgcagctat ataagaatta tcttttactt ccacaggagc
     7621 tataagattc acattacaac ccacaaaagc attgtctcca ataactgttc tgttcttttc
     7681 ctttccattg taatttactg taataacccc acaggctata ttagtattcc ttccaacagt
     7741 agtatcacct atataagaaa gatggggtat ctttgttttg tctccgatat ttgatttctt
     7801 caactcaacg aaatccccaa cttttacgtt cctgcctatc ctgttacccg gccttatata
     7861 tgcgaaaggc cctacggagg ttttatctcc aatagtgctt tctattatga cagtatttat
     7921 aatctctact tcatcaccta tagtagtaga agtaattcta ctacttgggc caattataca
     7981 ttcctcacca atttcagtac tgccttcaat tattgttccc ggataaataa ccgtatccat
     8041 gcctattttt actccatcat ctataaatgt agagtttgga tcaataattg taaccccatt
     8101 cttcatatgg tattcaagaa ttctcctttt catcacttcc gatgcctggt acaattgaat
     8161 cctatcatta actccaagta tctccatagg atttttaacc tcacaggagc atacctttca
     8221 tccctttttc aaaagtatcc ccattgt
//
LOCUS       DAOSDD010000202         8218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3237_length_8218_cov_5.031361, whole genome shotgun sequence.
ACCESSION   DAOSDD010000202 DAOSDD010000000
VERSION     DAOSDD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3237_length_8218_cov_5.031361"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK733_15045"
     CDS             complement(<1..566)
                     /locus_tag="PK733_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ11889.1"
                     /translation="MKRLKVGIVGIGGIGKGVHLTGWSQNPNTEVIALCDIRLQRAQE
                     AAEQYHIKHVFENYEDMLAMDEIDIIDICTPNYLHSKIAVKAMEKGKHVFCEKPDAIS
                     PEEALKMKEASEKSGKMLMVMRNNRFADFAQYLKKYIEDGNLGEIYAAKCGWIRRRGI
                     PGKGGWFTTKAQSGGGPLIDLGVHMIDLA"
     gene            complement(598..1494)
                     /locus_tag="PK733_15050"
     CDS             complement(598..1494)
                     /locus_tag="PK733_15050"
                     /inference="COORDINATES: protein motif:HMM:NF027034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOJ11890.1"
                     /translation="MKFIRKYFENKIEMIYKLILPNLMIKEIGCSVNEKKNIENWDQY
                     ILASRLIGPPGAHSRENILGKIAKFVEKQQCGEDGQYRLNLPILKRQEILDKVIRVCG
                     ISNEPEYLEGQDWVSIDAFKNGILPAVNIAGKIARESGTILMATGHPESLPPAYTYIG
                     KCLVNEGAHLYNPSIYSIPSTKHNQWLGSICGVTMVFKTTEVKHTHDDCRLYEILLKE
                     KVRPDLVIADHGMAGAAIRSGIPTIAIVDTNDIELIVASCTYPDRVFPVPLNDNAGIK
                     PTMALAYYFEHLIYKNKVGCKS"
     gene            complement(1667..3658)
                     /locus_tag="PK733_15055"
     CDS             complement(1667..3658)
                     /locus_tag="PK733_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOJ11891.1"
                     /translation="MQTGKPIMVNESEMCEMGHGLSASAFMLDWHGNGGRDILYTLHP
                     NYLGCGVYLYKEDIIRNDNIPVYNLPIKLEGLTGWNAIPLQTISKNRFNILTYGKHPR
                     KINGYVNIEKYPGWLKLYKNTGTTEKPQFVTEAEPIKVKGLSLDEAIKTESIGSINTA
                     GLFTGNNTDLIITTSKPNNIYWPGGISGWEHGDEHPDVGFGRGYNKDGTWKGKPVTKN
                     IYILRNNGTDETLCYGAPELIYSFEAFDSYVDAAFLDVDKDGVAELVIRKDIDRMYCL
                     KLIDGNVAGKPIKMECSPLKRGYFQTSLCICDLDKDKEPEFLVTGNPGVVLWIDFKDG
                     KWEEQKPLKTWGGYVRGETLSVPCMVDLDGDGDLDIIVGDSSGYIWYYENMSSKKNIF
                     NFKSGRKLIVDGEEIHHQAGSQGSIQGPNESRWGYTNPVVYDWDGDGLLDIITNDIKG
                     EYLFYKNIGSKEKPLFKKPLPFMLYGKPLKNAWRSRPAIWDKDTIVIATIDGLLQFFH
                     KGKQVESLRQDPLSVEEGKILRYADGTAIKASGSCGHTGRVVLSACDWDNDGRTDLIA
                     GTMRSITKLFNLHFPKIATLMWLKNVGTNITPIFERPRLITLKDGSFIDLDHHKCSPW
                     CVDLDGDDELDIVCGAEDGKVYSWFRKDLKWDWDPETVF"
     gene            complement(3691..4740)
                     /locus_tag="PK733_15060"
     CDS             complement(3691..4740)
                     /locus_tag="PK733_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOJ11892.1"
                     /translation="MECKVIKKEFIFEDDRPYKSCHASTILYLPGGEMISAWFGGTHE
                     GADDVAIWFSRRTQTSWTYPVKAADIEGIPHWNPVLFKRNDGTILLFYQDGHKIRDWK
                     SKLTMSKDCGKKWSEPKEIVEGARGGRGPIKNKPIILADGTWLAPCSCEIGPWNAFVD
                     ISTDEGISWTKSKVIPLVRDPSDNSPYFDNNESTINLKNMPELPGQYFVVKGRGVIQP
                     TLWESDPGNVHMLLRSCEGLILRSDSRDSGKTWSPVYSAGLPNNNSGIDIVKLDNGTL
                     VLVCNPVGKDWGPRTPLVIYVSCDNGQIWENIFTLENEPGEFSYPAIIAKGNEVFITY
                     TWRRERISFWHMVIE"
     gene            complement(4794..5747)
                     /locus_tag="PK733_15065"
     CDS             complement(4794..5747)
                     /locus_tag="PK733_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ11893.1"
                     /translation="MCKILVTATNYSTQCKKAKELLEHNGHEVIENMFGRPLTFDEIK
                     NVICDVDGVIAGVDAWDENIFVLAPKLKIISRFGIGVDNIDIVKAKEYGITVTNARGS
                     TNAVAELTIGLMLSVLRDIPNLDSSTRKGYWERFVGRELSGKKVGLVGFGHIAQSVAQ
                     KLRSFNVEICAHDKYPDAKKAADLGVSLVSFENILEECDIVSLHIPSLKETYHIMGEK
                     EFQMMKEGAVFINTARGALVDEKALYAALKSGKIAAAGLDVYEKEPVDINNPLLKLEN
                     VIATPHTAAETYETIDKVSMITAKAIIDYFNGKEPENVLNA"
     gene            complement(5782..5904)
                     /locus_tag="PK733_15070"
     CDS             complement(5782..5904)
                     /locus_tag="PK733_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11894.1"
                     /translation="MEELVAGKSHESLLLNNINHPNDYGHWLYFKALERLLSEE"
     gene            complement(5955..8087)
                     /locus_tag="PK733_15075"
     CDS             complement(5955..8087)
                     /locus_tag="PK733_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ11895.1"
                     /translation="MVKKYTPINEKFPHFIHGADYNPDQWKDTPEIWDEDMRLMKLAH
                     CNVMAVGIFSWACLEPEEGQFQFEWLDEIMGKLADNGLYAILSTPSASRPAWMAEKYP
                     EVLQIRPNRVRALFGERQNQCYTSPVYREKVRLINQKLAERYKDHPALLVWHVSNEYH
                     GYCNCGLCEAAFRQWLKEKYDNDINKLNHAWWSGFWGHTYNNWNQIEAPAPHGSKTVH
                     GHNLDWKRFTTHQTIDFYRNEIAPLKEITPNIPVTTNFMGSYSDLNYWEFAPYVDVVA
                     WDNYPLWHSGQPDWEVGSKTAFIHDINRCIKGGKPFMLMESTPSLTHGISKLKRPGMH
                     LLSSIQAVAHGSDTVQYFQWRKSRGGFEKFHGAVVGHSGHENTRVFKDVAGVGKTLEK
                     LNKIVGTTVRPDVAIIYDWENRWALDDIMAIGNHRKDYELTCQMHYKAFWEKGVPVDV
                     IEMTSDFSSYKLLIAPMLYMVRPGVAERIEKFVENGGTLITTYWSGIVNENDLCFLGG
                     FPGPLRKVTGIWAEEIDTLYDNELNRAVLCNDNELELNNEYEIRGICELIHAETAKVL
                     ALYKDDFYAGRPAFTVNNFGKGRAYYIAFKSNGDFLVDFYSELIKELKILKTIDVELP
                     DGVTAQLRTDEQRDFIFLMNFANEEKSIDVSSIEYIDVLNGEKITGTIHLSGYGIKIL
                     ERLRNNQSRIVVQTAEALGGKSSCKNII"
BASE COUNT         2286 a         1886 c         1231 g         2815 t
ORIGIN      
        1 gccaggtcta tcatatgtac ccccaggtct atcaaaggac ctcctcccga ttgggcttta
       61 gttgtaaacc atcctccctt tccgggtatt ccgcgccttc ttatccatcc acattttgct
      121 gcatatattt cgcctagatt tccatcttct atgtactttt tcaaatattg tgcaaaatcc
      181 gcaaaccggt tatttctcat taccataagc atcttaccgg atttttcact ggcttctttc
      241 atttttaatg cttcttccgg actaatggca tcaggttttt cacagaaaac atgctttcct
      301 ttttccatag cctttacagc aattttagaa tgaagataat ttggtgtgca aatatcaata
      361 atatcaattt catccatagc aagcatatct tcgtaatttt caaatacatg ctttatatgg
      421 tattgctccg ccgcttcctg tgccctttgc aatcttatat cacataatgc aattacttcc
      481 gtattcgggt tttggctcca ccctgtaagg tgtacaccct tgccaatacc cccaataccc
      541 acaatcccta cttttagcct tttcataata taaccccctt aatgatttct agatagctta
      601 agatttgcac ccaaccttgt tcttatatat taaatgctca aaataataag ccaacgccat
      661 agttggtttg attcctgcgt tatcgttcaa aggcacagga aacaccctgt ccggatatgt
      721 acaggaagcc acaattaact ctatgtcatt tgtatctaca attgcaatag tagggatacc
      781 tgaccggata gcagcacctg ccattccatg atctgcaatc accaaatctg ggcggacctt
      841 ttcctttagt aaaatttcat acaatctgca atcatcatga gtatgcttaa cttcagtagt
      901 tttgaaaacc attgtaacac cgcatatact tcccagccat tggttgtgct tggtagatgg
      961 aatagagtat atggatgggt tataaaggtg agctccctca ttcaccagac actttcctat
     1021 ataggtataa gcaggaggaa gcgattccgg atgtccggtt gccattagaa tagtacctga
     1081 ctcccgggct atcttacccg caatatttac agcaggaagt attccgtttt taaaagcatc
     1141 gatggatacc caatcctgcc cttccagata ttctggttca ttcgaaattc cacatactct
     1201 gataacctta tccaagattt cctgacgttt caaaataggc aggttcaatc tatattgtcc
     1261 atcttcccca cattgctgct tttcaacgaa ctttgcaatt ttccccagaa tattctctct
     1321 ggaatgagcc cctggcgggc ctatcagacg tgaagccaaa atatattgat cccaattttc
     1381 tatatttttc ttttcattta ccgaacaacc tatttctttt atcattaaat tgggcaggat
     1441 aagtttataa atcatttcaa ttttattttc gaaatacttt cttataaatt tcacccttat
     1501 tccaccccct cagcaagccc tgaaaacttt aaagctcgtt gtctgtaaga tttaggttgt
     1561 atatgtaagg tattatactt ttatctatct cactatccgt attatgcttt atatcatctt
     1621 actttattac catatcatat tatgtttgct atgtcaatat taattattaa aacactgttt
     1681 caggatccca atcccatttt aagtcttttc taaaccatga atatactttc ccatcctcag
     1741 ctccacatac aatatccagt tcatcatctc catctaaatc aacacaccag ggtgagcatt
     1801 tatggtgatc aagatcgatg aaagagccat cttttagagt aattaacctg ggtctttcaa
     1861 atatgggggt aatattagta ccaacatttt tgagccacat caacgttgct attttaggaa
     1921 aatgtaggtt aaatagtttt gttatacttc tcattgtacc tgctattaaa tctgtcctgc
     1981 cgtcattgtc ccaatcacat gcagaaagca ccacacggcc tgtatgaccg caagaacctg
     2041 atgctttaat tgcagttccg tcagcatatc ttagtatttt accttcttca actgatagtg
     2101 ggtcttgcct taatgattct acttgtttac ctttatgaaa gaattgaagt aatccgtcta
     2161 tagttgctat aacaattgta tccttatccc atattgcagg acggctccgc caggcatttt
     2221 tcagcggctt gccatataac ataaatggta aaggcttttt aaaaagaggt ttctcttttg
     2281 atccaatatt tttataaaat agatactccc cttttatatc atttgtaata atatccaata
     2341 acccatcacc atcccagtca taaactacag ggtttgtgta gccccatctt gactcattag
     2401 gtccctggat tgagccttgg gaaccagctt gatggtgtat ttcctctcca tctacaatta
     2461 atttccttcc tgatttaaaa ttaaaaatgt tcttcttgct tgacatattt tcataatacc
     2521 aaatataccc tgaagaatct ccaacaataa tatccaagtc cccatcccca tcaaggtcaa
     2581 ccatgcatgg tacggataag gtttctcccc tcacatatcc tccccatgtt tttaaaggtt
     2641 tctgttcctc ccactttcca tctttaaaat caatccataa tacaacacct ggattacctg
     2701 taaccaaaaa ttcaggttcc ttatccttat ctaaatcaca aatacacagg gaagtttgga
     2761 agtaacctct tttaagaggg ctacactcca tttttatcgg ttttcctgca acattgccat
     2821 ctataagctt taaacaatac atgcggtcaa tatctttacg gattaccaat tcagccaccc
     2881 cgtctttatc aacatccagg aaagcagcat caacatatga gtcaaatgct tcaaaagaat
     2941 atataagttc aggagctcca taacacagag tttcatcggt cccattattt cttaagatat
     3001 atatgttttt ggtaacaggt ttacctttcc aggtaccatc tttattatat ccccttccaa
     3061 agccaacatc tggatgttca tccccatgtt cccacccgga tattccacca ggccagtaaa
     3121 tgttattagg tttcgatgtg gtaatgataa ggtcggtatt atttcctgta aaaaggcctg
     3181 ctgtgttaat agaacctata ctttctgttt taattgcttc atccaatgat aaacctttta
     3241 cctttattgg ttcagcttcc gtcacgaatt gcggtttttc tgttgttcca gtatttttgt
     3301 atagttttag ccatccaggg tatttttcaa tattgacata tccattaatt ttccttggat
     3361 gttttccata tgttaaaata ttaaacctgt ttttactgat tgtttgaaga ggaatagcat
     3421 tccaacctgt caacccttcc agttttattg gtaaattata cactggaata ttatcattgc
     3481 ggattatatc ctctttatat aaatatacac cacatcctaa ataattagga tgtaaggtat
     3541 atagaatatc ccttcctcca ttcccgtgcc aatccagcat gaaagcagaa gcggataatc
     3601 cgtgtcccat ttcacacatc tcagattcgt tgaccattat tggtttccct gtttgcattt
     3661 aatcctcctt ttttaccctg cactaagtac ttactcaatc accatgtgcc aaaatgatat
     3721 tctctcacgt ctccaggtat atgtaataaa aacctcgttg cctttagcaa taattgcagg
     3781 atatgagaac tctccaggtt cattttcaag tgtaaatata ttttcccata tctgcccatt
     3841 atcacaagac acatatataa ctaaaggagt tcttggtccc cagtctttac ctacaggatt
     3901 gcatacaagc actaatgttc cattgtccag ctttacaata tcaattccgc tattattgtt
     3961 gggaagacct gcagaatata caggtgacca ggttttcccc gaatccctgg aatcacttct
     4021 taatatcaat ccttcacaac ttcttaaaag catatgtaca tttcctggat cggattccca
     4081 taatgtaggt tgaattactc cccttccttt cacgacaaaa tattgccctg gtaattcagg
     4141 catgtttttt aagtttattg tggattcatt attatcaaaa tacggagaat tatccgaagg
     4201 atcacgtact aaaggaataa ccttgctttt tgtccaagaa ataccttcat ctgttgaaat
     4261 atccacaaag gcattccacg gacctatttc gcaggagcaa ggagcaagcc atgtaccatc
     4321 tgccagaatt attggtttgt ttttaatggg tccccgtcct cccctggcac cttccacgat
     4381 ttctttaggt tcggaccact ttttaccgca atctttggac attgttaatt ttgatttcca
     4441 atcacgaatt ttgtgcccgt cctgataaaa aagaagtatt gtaccatcgt ttcttttaaa
     4501 cagtacagga ttccaatgtg gtatcccttc tatatcagca gctttaacag gataggtcca
     4561 gctggtttgt gtacgtcttg aaaaccatat agctacatca tcagctcctt catgtgtccc
     4621 cccgaaccat gctgaaatca tttctcctcc gggaagatat agaatagtag acgcatggca
     4681 gcttttatac ggtctgtcat cttcaaatat aaattctttt ttaatcactt tacattccat
     4741 tttcattacc cccagttaat atcaatttaa atatgcataa ttttgaatgt tgactaggca
     4801 tttaaaacat tttcaggttc tttgccatta aaatagtcaa ttatagcttt tgctgttatc
     4861 atgctgactt tatctattgt ttcataagtt tcagctgctg tatggggtgt tgcaataaca
     4921 ttttccaatt ttaataatgg attatttata tctacaggct ccttttcata tacatcaagc
     4981 cctgcagctg caattttacc cgactttaag gcagcataaa gagctttctc atcaacaagt
     5041 gctccacgag cagtattaat gaaaactgcc ccctctttca tcatctggaa ttctttttca
     5101 cccataatat gataggtttc ttttaggctg ggaatatgaa ggcttacaat atcacactcc
     5161 tctaaaatgt tttcaaaaga taccagggat acccccaaat ctgctgcttt tttagcatca
     5221 ggatatttat catgtgcaca tatttctaca ttaaaactcc ttaatttttg tgcgactgat
     5281 tgggctatat gtccaaatcc tactaatcct acttttttgc ctgacagttc gcggcctaca
     5341 aatctttccc agtacccttt tctggtagaa ctatcaagat ttggtatgtc tcttaaaaca
     5401 cttagcataa gccctattgt aagttctgcg actgcatttg ttgaaccccg ggcattagtt
     5461 acagttattc catactcttt cgcctttaca atatcaatat tatcaacacc tataccgaat
     5521 cttgaaatta tcttaagttt tggtgcaagc acgaatatat tttcatccca tgcatctact
     5581 cctgcaatta caccatctac atcgcatatt acatttttta tctcgtcaaa tgtcagcggt
     5641 ctgccaaaca tattttcaat aacttcatgt ccgttatgtt caagaagttc cttagccttt
     5701 ttgcattggg tcgaataatt tgtagccgtt accagtattt tacacattta tacctgacac
     5761 ctccaatttt ttgttttcct cttactcttc cgaaagtaat ctttcaagtg ctttaaaata
     5821 cagccaatgc ccataatcgt tgggatggtt gatattgttt aacaatagac tctcatggct
     5881 ttttccggca actaattctt ccattcaaat taactgtacc cattcttctt ttataaataa
     5941 gacactttct tttatcatat tatattcttg catgatgatt ttcctcctaa cgcttctgca
     6001 gtttgaacga caattctact ttggttattc cttaatcgtt ccagtatttt aatgccatat
     6061 ccagaaagat gtattgttcc agtaatcttt tcaccattca acacgtcaat atattcaata
     6121 ctacttacat caatgctctt ttcttcattt gcaaaattca taagaaatat aaaatctctc
     6181 tgttcgtcgg ttctcagctg tgctgttacc ccgtccggca gttcaacatc aatagtcttc
     6241 agaattttga gttcttttat aagttcgctg taaaaatcca ccaggaaatc tccattgctt
     6301 ttaaaagcta tataataagc gcgccctttc ccaaaattat tcacagtaaa agccggcctg
     6361 cctgcgtaaa aatcatcctt atacaaggca agtactttag ccgtttctgc atgaatgagt
     6421 tcgcaaatac ctcttatctc gtattcatta tttagctcaa gctcattatc attacacagg
     6481 acagcacggt ttaactcgtt atcatacagg gtatctatct cctctgccca tatccctgta
     6541 acctttctta atggacctgg aaaacctccc aggaaacata gatcattttc attcactata
     6601 ccgctccaat aagttgtaat aagagtgcct ccattttcaa caaatttctc tatacgctct
     6661 gcaacccccg gcctaaccat atataacatt ggtgcgatga gtagtttgta acttgagaaa
     6721 tctgaggtca tctctattac atctacaggc acacctttct cccaaaatgc tttataatgc
     6781 atttggcagg taagctcata gtcttttcta tggtttccaa tagccataat gtcatctagt
     6841 gcccacctgt tctcccagtc ataaataata gctacatcag gtcttaccgt tgttcctact
     6901 attttattca acttttcaag tgttttacct acacccgcta catccttgaa aactcttgta
     6961 ttctcatgcc cactgtgtcc cactacagca ccatggaatt tttcgaaccc acctctgctt
     7021 tttctccatt gaaaatattg tacagtatca gaaccatggg caactgcctg aatagatgac
     7081 aaaagatgca taccaggcct cttaagcttt gaaattccat gggttaggct aggagtgctc
     7141 tccattaaca taaaaggttt tccacccttt atacatctgt taatatcatg tataaaagca
     7201 gttttgcttc ctacttccca atcaggctgc ccgctgtgcc acaaaggata gttgtcccat
     7261 gctactacat caacatatgg ggcaaattcc caatagttta gatcagagta gctgcccata
     7321 aaattagtcg ttacgggtat attaggtgtt atttccttta aaggtgctat ctcatttcta
     7381 tagaaatcaa tggtctgatg agtagtaaaa cgcttccagt caaggttatg tccatgcacc
     7441 gtttttgaac catggggagc cggtgcttct atctggttcc agttattata agtgtgaccc
     7501 caaaatccgg accaccaggc atggtttagt ttattaatat cattgtcata tttctctttc
     7561 agccattggc ggaaagcagc ttcacaaaga ccgcaattac agtatccatg gtattcattt
     7621 gatacatgcc atactaaaag tgcaggatga tccttatatc tctcggccag cttttgattt
     7681 atcagccgaa ccttttcacg atatacggga gaggtataac attgattctg gcgttcccca
     7741 aagagtgctc ttactctatt tggccttatc tgaagcacct caggatattt ttctgccatc
     7801 catgcaggcc ttgaagcact gggagtagaa agaatagcat ataaaccgtt atctgctaat
     7861 ttacccatta tttcgtctaa ccattcgaac tggaactgac cttcttccgg ttccagacat
     7921 gcccatgaaa aaatgcctac agccattaca ttacagtgag ccagcttcat aagacgcatg
     7981 tcttcatccc atatctctgg tgtgtctttc cattgatcag gattgtaatc ggctccatgt
     8041 ataaaatgtg gaaatttttc gtttatagga gtatattttt taaccatcat ctacacccaa
     8101 gtattatcct ttctgcattt ttgcaatata ttttttccag gacctccttc tcaagatata
     8161 taccgtatat tctccatctt ccctgttttg gtatagagct tccagaatat ttaaagta
//
LOCUS       DAOSDD010000203         8190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3260_length_8190_cov_5.467486, whole genome shotgun sequence.
ACCESSION   DAOSDD010000203 DAOSDD010000000
VERSION     DAOSDD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_8190_cov_5.467486"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1099)
                     /locus_tag="PK733_15080"
     CDS             complement(182..1099)
                     /locus_tag="PK733_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOJ11896.1"
                     /translation="MSIDTNIGKKKIKAIKEYLLIIIGSIITAAGINIFLVPNKIAPG
                     GVSGIATVIYYLSGGGFPVGIIMLILNIPLFIMGTKYIGKRFIVRTFFGTILLSAIID
                     LSQPLTSSFIDGFLTRTESLYYAPDLLLYSVFGGLLMGIGLGLVFRSGATTGGSDLAA
                     KIVNHFIPGFTVGQILLFIDTGIIVFAAITFKSFILGLYAIVTLFIMSRVIDAIIEGV
                     DFAKAVYIISDKSDEIAEKIMYELDRGVTSLNGKGMYTGKDREVLLCVLYRGQLPQLK
                     QIVKSVDPIAFIILTDVREVLGEGFKEHD"
     gene            1361..3604
                     /gene="csaB"
                     /locus_tag="PK733_15085"
     CDS             1361..3604
                     /gene="csaB"
                     /locus_tag="PK733_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOJ11897.1"
                     /translation="MRVLHLIGGGDIGGAKTHVLHLVKELGKYVDVKIVSFRPGIFAD
                     EAKEMGIDVEVVKKGNVFKDIKRVAQIVKEGGYQIIHSHGAKANMIAIIVKRLTHVST
                     VTTVHSDYRLDYMHSIVKSLTFGIINTVSLRFMDFYIGVSKNFKEMLISRKFNPINIF
                     TVYNGVDFNSATKEYSREIFKEKYKTLLNNDDIIVGIAARLYPVKSISTLIDAAKVVA
                     DKNPKVKFIIGGDGEERQMLENKAASLGLLNNVFFTGWLNDPYELMGIVDISVLTSIS
                     ESFAYSILEGARFKKATISTCVGGIPDLIESGENGYLFNPGDHIKLAEYILELANDKE
                     KRDTMGEKIYQKARSMFSLDNMCRTQLNIYETILKRSAYTKETKLTYDLVIAGYYGFN
                     NIGDEALLMAIIQNLKVYIEDIKIIVLSNNPLETKLTYGVNAINRFNMIKIVKAMRKS
                     RMFMYGGGTLIQENTSTRSLIYYLGMIWAAKKAGLKVMLYANGIEPINKYINKRLTGN
                     IMNRVDIITLREQASKTELEKLSINKPEIIVTADPAFTLAPSKDSETERIFIDEGIGI
                     DGPFIGFSARKWDGYKKYVDVIARSADYVVDQYGAKPVFIPMQQPEDLLIIKSIVAKM
                     KHRGYIFENKYNVSQVMGITSKLDMLIGMRLHALIFAVSLGIPVVGLVYEPKVAWFLD
                     YIDQVDSSAGDVKTLEFERLKQVIDYTWLNKTEIKEHLKKVTVELKDKSVKNAKIAVA
                     VLTKGEG"
     gene            3765..4502
                     /locus_tag="PK733_15090"
     CDS             3765..4502
                     /locus_tag="PK733_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOJ11898.1"
                     /translation="MRNTINILGIPVDNITMSEALKIVNAFLNEDYVHTIFTPNSEIM
                     MAAQRDKELKNILINSNMLIADGAGVVLASKILGRKLPERVAGYDLVENIFSILGEKG
                     LKFYLLGAKPGVAEAAAINIISKHKGVEISGYHHGYFSKEENDRIIAEINSSGADILL
                     VALGAPRQEKWIQENKDKLKVKICIGVGGSLDGFAGKVPRAPDFFRKHNLEWLYRLYK
                     EPRRFWRMLDIPRFLFLVLKSRFSHSR"
     gene            complement(4533..5186)
                     /locus_tag="PK733_15095"
     CDS             complement(4533..5186)
                     /locus_tag="PK733_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11899.1"
                     /translation="MKLFKTTYLRKFFLILSFAVVLSILATHAVSGTTMEPGSDEDPL
                     VTKSYVDSAIGKLGNEVSSKIGILQSDITKPQDSVVLEKMAEKIMELSKTVYDLNKQL
                     EEQSKYSKFEVVEMEAGQKIILGDSSEIILRAGKALAIAGEKGDGLADITTDDKKNSL
                     VTDDIVPLNHLLLVSRDDGRGIKAVTKIWVLVKGDYTIIEDGNQAEDSNKVESKSSN"
     gene            complement(5211..5561)
                     /locus_tag="PK733_15100"
     CDS             complement(5211..5561)
                     /locus_tag="PK733_15100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOJ11900.1"
                     /translation="MVIKRGDMFYADLSPVVGSEQGGVRPVLIVQNDVGNKFSPTVIV
                     SAITSQINKAKLPTHVEINAQEYGIQKDSVILLEQIRTIDKKRLREKIGHLDDKLMEQ
                     VNDALSISFGLVDI"
     gene            complement(5565..5840)
                     /locus_tag="PK733_15105"
     CDS             complement(5565..5840)
                     /locus_tag="PK733_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOJ11901.1"
                     /translation="MAESRKILISLPESLLKEVDSIVSIEKTNRSEFVRKTIQLYIRE
                     RRKIEVRDRLKKGYQEMAEINIRLSEIWFDADNDLQLKYEQRLGELE"
     gene            complement(6195..7364)
                     /gene="alr"
                     /locus_tag="PK733_15110"
     CDS             complement(6195..7364)
                     /gene="alr"
                     /locus_tag="PK733_15110"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ11902.1"
                     /translation="MEYKLNRAWAEINLDNIAHNVREIRRIVNSRADILGVVKADAYG
                     HGAIEVVNTLLDNGVSHLAVSMLDEAIQLRLHGIDVPILILSYTDPVRAEEIIKYNVT
                     QTVFSHDLARALSDTAVKMGRKVKIHIKIDTGMTRVGFMPGYNAIKNVVEISKLPGII
                     IEGLFTHFASADEEDKSYTYTQFERFMSICTELQRIGVYIPVKHVANSAAIIDFPEMH
                     MDMVRAGIILYGHYPSDCVDKKKISLKPAMSLKANVILVKDVEPSTPISYGLTYTTRH
                     LSKIATIPIGYADGYSRLLSGKAEVLVNGDPAPIVGTICMDQCMIDVTNMKKEVKVGD
                     EVVLFGKQGGNEIKVEEIASLIGTINYEIISVMGKRIPRVYLHEGEIHNVLNYLI"
     gene            complement(7506..>8190)
                     /locus_tag="PK733_15115"
     CDS             complement(7506..>8190)
                     /locus_tag="PK733_15115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOJ11903.1"
                     /translation="AYIYNTVVKEAITVSMDDLGKGYISSGIINTFERYIEGKDVIAV
                     GPGLSVNEDIFRIVSWLIENSYIPLVLDADALNAVAKDVSILGKLRAPAVITPHPGEM
                     ARLMGTSIAEVQRDRIKAAGDFALRWNVITVLKGWHTVVALPDGRIFINTCGNPGMAT
                     AGTGDVLTGLIAGLMAQKLKPEDAAVVGVFIHGTAGDWVAGRIGEHGLIAGDLVEEIP
                     YVMNQISYR"
BASE COUNT         2586 a         1610 c         1348 g         2646 t
ORIGIN      
        1 aagaattgta attattttgt tcaaacactg attttgctct tccttcgctc agatatgggg
       61 aaactctcta gcataaaagg aatgcctgat acttgagaga agagtttccc ctgcccaact
      121 acagcacttt cacttattcc ccttaccaat attttcttca aataaattaa atgcacccat
      181 gctaatcatg ttccttaaag ccttctccca gaacctccct aacatctgtc aaaataataa
      241 aagctattgg gtcaacgctt tttactatct gcttcagttg gggtaattgt cccctataca
      301 atacgcataa aagcacttcc ctatctttgc cagtgtacat tcctttacca ttcagggagg
      361 ttactcctct atctagttca tacataattt tttcagcaat ttcatccgat ttatcagaaa
      421 taatatatac agcttttgca aaatccacac cttctataat ggcatcaata accctggaca
      481 ttatgaacag tgtaacaatg gcataaaggc ctaaaataaa gcttttaaat gtaatagcag
      541 caaaaactat aataccggtg tcaataaata acaggatctg ccctactgta aaccctggta
      601 tgaagtggtt tacaattttt gctgccaggt cacttcctcc agttgtagct cctgatctaa
      661 atacaagccc cagccctatt cccataagca aaccaccgaa tactgagtaa agaagaagat
      721 caggagcata gtacaatgat tcagttcggg taagaaatcc atctataaaa ctggaggtta
      781 atggttggga aagatcaata atcgctgaaa ggagtattgt tccaaaaaac gtgcggacaa
      841 taaacctttt tccaatatat tttgtgccca taataaataa aggtatattc aagatgagca
      901 taattatacc tacaggaaaa cctccaccgc tcagataata aattactgtt gcaataccac
      961 ttaccccgcc tggtgctatc ttatttggaa caagaaatat gtttattccg gcagcagtta
     1021 taatagaacc aataattatc aataaatatt ctttgatggc tttaattttc tttttgccaa
     1081 tatttgtatc aatactcata tgtatactcc atccggatgt attttttaat attatgtttc
     1141 atatttttat tatgcaccct gtattgtaat agtgtcaagg atatgcagtt gctaattttt
     1201 cataaccctt tattgacaaa tttcagctca taaaataaac tttaaaaaaa gcacataata
     1261 ctgtataata ttatacaaca ttatgcccgg gctttgattt agcagggtaa attattgata
     1321 tatacgatat tgaaattata agcatttatt aggagatgtt atgagagttc ttcatttaat
     1381 aggtggtgga gatatagggg gagcaaaaac ccatgtcctg caccttgtaa aggaattagg
     1441 caaatatgtg gatgttaaga ttgtaagctt ccgcccaggt atttttgcag atgaagctaa
     1501 agaaatgggt atagatgtgg aagtggtgaa aaaaggtaat gttttcaaag atataaaaag
     1561 agtggcacaa attgttaagg agggtggata ccagattatt cattctcatg gtgcaaaagc
     1621 taatatgatc gccataattg taaagcgatt gacacatgta tcaacagtta caacagtaca
     1681 tagcgactat agacttgatt atatgcacag cattgttaaa agtctcactt ttggcattat
     1741 taatactgtg tcattaaggt ttatggattt ctatataggt gtttcaaaaa atttcaaaga
     1801 aatgcttatt agcaggaagt ttaaccctat aaacattttt acagtatata atggtgttga
     1861 ctttaacagt gcaacaaagg aatattcaag ggaaatattt aaagaaaaat ataaaactct
     1921 tctcaataat gatgatataa tagttggaat tgccgcacgt ttatacccgg taaaaagtat
     1981 aagtacatta attgatgctg ctaaagtggt tgcggacaag aatcccaaag taaagtttat
     2041 cataggcggc gatggtgaag aacgccaaat gcttgaaaat aaggctgctt ctctgggatt
     2101 gcttaataat gttttcttta caggttggct gaacgatcca tatgaattaa tgggtattgt
     2161 agatattagt gtactcacct ctattagcga aagttttgca tattccatcc ttgaaggggc
     2221 aagatttaaa aaagctacaa taagcacctg tgtaggagga atacccgacc tcatagaaag
     2281 tggcgaaaat ggttatctat ttaatcccgg ggaccatata aaactggctg aatacatcct
     2341 tgaacttgct aatgataaag aaaaaagaga tacaatggga gaaaaaatat accaaaaagc
     2401 caggtccatg ttttctctgg acaacatgtg caggacacag cttaatatat atgaaaccat
     2461 attaaaaaga agtgcttata caaaagaaac caaattaaca tatgaccttg taattgcagg
     2521 ctattatggg tttaacaaca ttggtgatga agcactgctt atggcaatca ttcagaatct
     2581 taaagtatat atagaggata taaaaattat agtactttcc aataatcctt tggaaactaa
     2641 gcttacatat ggtgttaatg caataaaccg atttaatatg attaaaatag taaaggcaat
     2701 gagaaaatcc agaatgttta tgtatggtgg aggtactctt atccaggaaa acaccagtac
     2761 ccgctcactt atatattatc ttggcatgat ctgggcagct aaaaaggctg gtcttaaggt
     2821 tatgctttac gcaaatggga ttgaacctat aaataaatat attaacaaga ggttgaccgg
     2881 taatattatg aaccgtgtag acataattac tctgagggag caggcctcta aaactgaact
     2941 tgagaaactt agtataaaca agcctgaaat aatagtaact gcagatccgg cttttaccct
     3001 tgcaccatct aaagacagtg aaacagaaag aatttttata gatgaaggca ttggtattga
     3061 cggtcccttt atagggtttt cggccagaaa gtgggatggc tataaaaaat atgtagatgt
     3121 tattgcaaga tctgctgatt atgttgtgga tcaatatgga gctaaaccgg ttttcattcc
     3181 catgcagcaa cctgaagacc ttcttattat caaatcaatt gtcgcaaaga tgaaacacag
     3241 gggatatatt tttgagaata aatacaatgt atcacaggtt atgggtataa catccaaact
     3301 ggatatgctt ataggcatga gacttcacgc ccttatattc gcagtcagcc tgggaatacc
     3361 tgttgtcggt ctggtatatg aaccaaaggt agcatggttt cttgattata ttgatcaggt
     3421 agatagttca gcaggagatg taaaaacttt ggaatttgaa cgactgaagc aggtgatcga
     3481 ttatacatgg ttaaataaaa ctgaaatcaa agagcacctt aagaaagtca cagttgaatt
     3541 aaaggacaaa tctgttaaga atgctaaaat tgctgtagct gtgctaacta agggtgaagg
     3601 gtaatacaca atataataca agtgcatatt ttttgtacaa gaattttgaa aaataaattg
     3661 cacgcgcgac tgaaatgaat ttaagcccat aaaaactttt tcataaaaag ttttgttcct
     3721 gcaagggcga attcatttcg cacgcagcag aaatggagct taatatgaga aacacaataa
     3781 atattctagg tatacctgtt gataatataa ctatgagtga agccttgaaa atagttaatg
     3841 catttttgaa tgaggattat gtgcacacta tatttactcc aaattctgaa attatgatgg
     3901 ctgctcaaag agataaagag ttaaagaata ttcttataaa cagcaacatg cttatagctg
     3961 atggtgcagg ggttgtactt gcatccaaaa ttctggggcg gaaacttccc gaacgtgttg
     4021 cgggatacga tcttgtagaa aatatattta gtattttagg agaaaaagga ctgaagtttt
     4081 acctgttggg agctaaacct ggagtagctg aagctgcagc tataaatata attagcaaac
     4141 ataaaggtgt tgaaatcagc ggatatcatc atggctattt ttctaaagaa gaaaatgata
     4201 ggattattgc tgagattaat tccagcggtg cagatatact gcttgttgct ctgggagctc
     4261 caagacagga aaaatggatt caagaaaata aagacaaact caaggtcaag atctgtattg
     4321 gagtaggagg aagtctggac ggattcgcag gaaaagtccc ccgtgctcct gatttcttca
     4381 ggaaacacaa ccttgaatgg ctatacaggt tatataaaga accacgccgt ttctggagga
     4441 tgctggatat acccaggttt ttattccttg tccttaaatc caggttttcc cacagtcgat
     4501 gatgagccat aggatatctt tatatttact ttttaattac tgctcttgct ctctacctta
     4561 ttgctgtcct ctgcctgatt gccatcttct attatggtat aatcaccttt taccagtacc
     4621 catatttttg ttacagcctt tatgcctctg ccgtcatccc tggatactaa aagcaggtga
     4681 tttaagggaa ctatatcatc tgtaacaagg ctgttctttt tatcatcagt tgtgatatct
     4741 gccaatccat cacctttttc cccggctatg gcaagagctt taccggctct taaaattatt
     4801 tccgaactgt caccaagtat tattttctgt cctgcttcca tttcaacaac ttcaaatttc
     4861 gaatattttg attgttcttc aagctgttta tttaaatcat acactgtctt acttagttcc
     4921 attatctttt ctgccatttt ttccagaact acgctatcct gaggcttcgt aatgtctgat
     4981 tgtagtatac cgatttttga acttacctca tttccaagtt tgccaattgc agaatccaca
     5041 taactttttg taacgagggg gtcttcgtca cttcccggtt ccattgtggt accgctgaca
     5101 gcatgtgttg caagtatact taaaactacc gcaaatgata atatcaaaaa aaattttctt
     5161 aaatatgttg ttttaaataa cttcatctta tgtaaacctc acttaaacaa ttatatgtca
     5221 accagcccaa aacttatact taaagcatcg tttacctgct ccataagttt atcatccagg
     5281 tgcccgattt tctctctcag cctcttttta tcaatagtcc taatttgctc aagcaatatt
     5341 accgaatctt tttgtatacc atattcctgt gcattaattt caacatgcgt aggaagcttg
     5401 gctttattaa tttgtgaagt tattgctgat acaataaccg ttgggctgaa tttatttcca
     5461 acatcatttt gtacaatcag tacaggcctt actccaccct gctcggaacc aacgacagga
     5521 ctcaggtctg catagaacat atctcctctt tttattacca atttctactc caactcccca
     5581 agcctttgct catattttag ctgcaggtca ttatccgcat caaaccatat ttcagataac
     5641 cttatgttta tctcagccat ctcctgataa ccttttttca acctgtccct cacctcaatc
     5701 ttccttcttt cccttatata taactgtatt gtcttccgta caaactcact cctgttagtc
     5761 ttttctatag aaactataga atcaacttct ttaagcagac tttctggaag actgattaga
     5821 attttacgtg actctgccaa aattaacccc cccataagga acatataacc ggcttaggta
     5881 taaatagcca acaagtatca tcattcaatt gaagccgctt gttttagttt atttaagttt
     5941 atccttcacc agcaagttga acggcttttc cctacatatt acattaaatt attaaaaaaa
     6001 cacttcttct gctataatat actataatta tatattcttc aatatgtaca ggtcaaatta
     6061 aataaatgtt accgtttcaa cttaaattga ataaaagctg ctataatgat taattcacct
     6121 acttattccg attctaactt tcccgatttt ctaatctaaa tactgtaatc ccataatttt
     6181 acttttctat acaatcatat aaggtaattt aagacattat gaatttcccc ttcgtgcaaa
     6241 tatactctgg gtatcctctt acccataaca cttattatct cataatttat tgttcctatt
     6301 aaagatgcaa tttcttcaac ttttatttca ttgcctcctt gttttccaaa taaaaccacc
     6361 tcatctccta cttttacttc tttcttcata tttgttacat caatcatgca ctgatccatg
     6421 caaattgtcc caacaatagg tgccggatct ccattaacca aaacttccgc ttttccgctt
     6481 aaaagcctgg aatatccatc tgcatagcct ataggtatag ttgctatttt gctcaagtgt
     6541 cgggttgtat atgtaaggcc gtaacttatt ggtgtactcg gctctacatc tttaacaaga
     6601 atcacatttg ccttcaaact cattgccggc ttcaggctta tttttttctt gtcaacacaa
     6661 tctgaaggat aatgcccata taatataata cccgccctta ccatgtccat atgcatctct
     6721 gggaaatcta ttatagcagc actattggca acatgcttca caggtatata tacgccaatt
     6781 ctttgaagct ctgtacaaat gctcataaac ctttcaaatt gggtatatgt atagctttta
     6841 tcttcttcat cagcagatgc gaaatgggta aaaaggcctt ctattataat tcccgggagc
     6901 ttgcttattt ctacaacatt ttttattgca ttataaccgg gcataaaacc taccctggtc
     6961 atacctgtat caatttttat gtggattttc actttacgcc ccattttcac tgccgtatca
     7021 gataaggctc tagctagatc atggctgaac actgtctgtg ttacattata tttaataatt
     7081 tcctcagccc ttacagggtc cgtataactc aaaataagta taggtacatc tatcccgtgc
     7141 aagcgcagct gtattgcttc gtccagcatt gataccgcaa gatgcgacac accattatcc
     7201 agcagtgtat taacaacctc tatagcgcca tgtccatatg catcagcttt tactacacct
     7261 agtatatcag ctctgctatt tacaattctc cttatctctc ttacattatg tgcaatatta
     7321 tcaagattta tttcagccca tgctctattt aatttatatt ccataattaa ttcacctgtt
     7381 ttattagtta atttctacta tatatataac gcaaaaatga ttctacaaag aacaaattgg
     7441 atttttgcgt attcgctaag gtaacataag cacacaaaaa ttttatatgc attttctttt
     7501 gtgcgttatc tatagctaat ttggttcatc acataaggga tttcctcaac aagatctcct
     7561 gcaataagcc catgctcccc aattcttccg gctacccagt cacctgcagt gccatgtata
     7621 aagactccta caactgcagc atcctcaggt ttcagcttct gagccatcaa cccggcaatt
     7681 aatcctgtca gtacatctcc tgtgcctgca gttgccattc ccgggtttcc gcaggtatta
     7741 atgaaaattc tcccgtcagg taatgctacc accgtatgcc agcctttaag aacggttatt
     7801 acattccatc tcagtgcaaa atctcctgca gctttaatcc tgtctctttg aacttctgca
     7861 atacttgtcc ccataagtct tgccatttct ccggggtggg gtgtgataac agccggagct
     7921 cgcaatttac ctaatatcga aacatcttta gccacagcgt ttaatgcatc agcatccaat
     7981 actaaaggta tatacgagtt ttctataagc cagctgacaa ttctaaaaat atcttcattt
     8041 accgatagcc ctggacctac ggcaataaca tcctttcctt caatatatct ctcaaatgta
     8101 ttaatgatcc ctgacgaaat atatcctttc cccagatcat ccatagaaac agttattgct
     8161 tcctttacaa ctgtattata aatgtaagca
//
LOCUS       DAOSDD010000204         8078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3304_length_8078_cov_5.406955, whole genome shotgun sequence.
ACCESSION   DAOSDD010000204 DAOSDD010000000
VERSION     DAOSDD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3304_length_8078_cov_5.406955"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1268
                     /locus_tag="PK733_15120"
     CDS             36..1268
                     /locus_tag="PK733_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11904.1"
                     /translation="MDIILNDSKKWYRAYKKLDDEKKYQYVLETIDCEIPVKFFDNID
                     FIGIIIHVFDYLKNEKQHEKMIELYDKAYRWKENLDEWFYCDKFLINYYLYYNNITEV
                     KKHLDSFLSNPGESLDIFIPIFDKLVYYGHSDLAMDISLHMFDKVKEAHELVAGSEYE
                     FGRIIYMEKLQSIYNDLKRNIPVHRKAIIEYFEKYGYDIELDIDMVMDILSPMNDKKP
                     DYDNFQKNKSGFSDFSYALMIKFCRYMLDTKNISFCTSGDIWFIALHSFKADLPAKTP
                     KTNFDNVFELDEDKYDEEISGRMGFISNKYTCGFAVAWGMIYVYDFLYKYGYISENVY
                     YDALEVIDGIKVEIIKGYANSLWEYDFVYAWKKPDSISDEEFIIEKNLFDDSFKVQVK
                     IVDDLTFNDIIEDDNSTE"
     gene            1962..2759
                     /locus_tag="PK733_15125"
     CDS             1962..2759
                     /locus_tag="PK733_15125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ11905.1"
                     /translation="MKYLIINGSSRKGNTWKLAELIKNSIEQISPESSFEEIQLGELE
                     LPFCVGCSLCFRKGHQYCPHYSIMKTLIEKIEECDGLIFSVTTFYMQPTAMTKNLIDH
                     FCFMLHRPHFFDKKAIVVSTVGAVGGKQAVKYLAGTLKGMGFNRCYRFPLSVYSWNNY
                     NPDIKAKTKCRQLAFKFHKDVSSRKMLAPSFSVLIPYNLFRGMSIWYAKGTEYETEDG
                     VYWVEPQRAKYTYDKSISVPLFKKMFGYIFYHLGKTIGKFATVTYKK"
     gene            2790..3314
                     /locus_tag="PK733_15130"
     CDS             2790..3314
                     /locus_tag="PK733_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11906.1"
                     /translation="MHQLAKFSHFGRALGLFLPTSTHRATSAAHGKDSVVRFVAVANA
                     EYINKLIRSSLTLLKERLLHLLKTLEGFEKDNDCYCMIHFDYNDGNYSIDFDTGQITV
                     YDFDNSLFGWYMYNLADLWGNGQRVIEEPIYGILIYLFYRNFGLDLHNIPKCFEDFKL
                     TFHFGGLYKIISDE"
     gene            3869..4495
                     /locus_tag="PK733_15135"
     CDS             3869..4495
                     /locus_tag="PK733_15135"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOJ11907.1"
                     /translation="MKTNKDRVQWLVLMGVAGTVFYFLHVILGEINYPGYHPLEQAVS
                     DLTAATAPSREIASAYSTVYAFFTVTACTLLCVFYQGRVNRIFRLGIYLFTVMEWISA
                     IGYTLFSLSSSGYAGTFQDVMHMVVTAFVVLLSITSMILIAVGCFRSKVHKRFGIFTI
                     AVLGIMAFGSIATGIVPVGYFGIAERLSVYSVVVYGAVLSLLTFKHSI"
     gene            5057..5548
                     /locus_tag="PK733_15140"
     CDS             5057..5548
                     /locus_tag="PK733_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ11908.1"
                     /translation="MAYKSYRPEDFETVVIKHENRLYRTALAIIGTMSDAEDIVQEAF
                     LRAYEKAPEFESEEHEKAWLIRVTVNICNSRLRSPWRKRTVPLLDSYPASEPEQHELL
                     EQIMALPSKYRTVIHLFYYEGYSIKDISGLTGQKESTVRSNLTRARQKLKSVLKEDDY
                     ESI"
     gene            5535..6830
                     /locus_tag="PK733_15145"
     CDS             5535..6830
                     /locus_tag="PK733_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11909.1"
                     /translation="MKAYNEYMNKIKVSSTLHQRLVSRTDSARSKRCSIMIKRYVAAF
                     ACLALVLLGVFTMPKLIQHNVTPIPGDNPRVLQPSADTSTPDASSKYALVFNKAEEQL
                     ANDIGIPGHFWQELTADELNTVFPGLTDTHTVTAAANFQTDENGVALFNIDAHIVSAS
                     GFKTYIQIAPDKVIVDYRLEGETKTSDVLGTAVTAGYFETKPNSKGFRNVIYFAAFKL
                     SDVAYYVELGGAEAEKEALKNEISELIGLLIEGGAADLDVFHPVVPELREDQLSLDEA
                     RADADYGAYLPTTLPDGFVFEDALRYINQEHNALHVNWTKGMGYINWRVSLLDDNDKT
                     RITSVADTKNYDLTLYPIPRADSVPDELREIVNDPIFLADELTLEAVQARTYEVSDSG
                     DELSPRMRFSVLFGDILVELSIKGASSEVMFDILQQIIK"
     gene            complement(6961..7719)
                     /locus_tag="PK733_15150"
     CDS             complement(6961..7719)
                     /locus_tag="PK733_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOJ11910.1"
                     /translation="MNYIEGVVVTATEFKQNFGKFMDFIEQQNDVVITKNGIKTARLT
                     PYVTDIEQYFIARENALDYQYGGKKVSYEEFIEITEKSTLRMELINGEIYLLGSPNIG
                     HQEILGRLYLIFSEYFKSKKCRVFLAPFDVHFKKKDIKEPDVMQPDVLVACDLENNVT
                     EKERYMGTPTLTIEILSDSTRSKDMIDKLNTYMLSGVKEYWIIDTKQESILVYNFANY
                     EIDRFKVFEKGYVATSLVFQGLSMNVEDLFRDLI"
BASE COUNT         2566 a         1365 c         1651 g         2496 t
ORIGIN      
        1 cttagggaat taattatcta gaagggaaat agattttgga tattatatta aatgactcaa
       61 aaaagtggta tagagcttac aaaaaattgg atgatgagaa aaagtatcaa tatgtattgg
      121 aaacaataga ttgcgaaatt ccggttaagt tctttgataa tattgatttt ataggtatta
      181 tcattcacgt atttgattat ttgaaaaatg aaaaacagca cgaaaaaatg attgaactat
      241 atgataaagc gtaccggtgg aaggaaaacc tggatgaatg gttctattgt gataaattcc
      301 taataaatta ttacctttat tataacaata ttaccgaggt aaagaagcac ctggacagct
      361 ttctgtcaaa tccgggagaa tccttagata tttttattcc catattcgat aaactggtct
      421 attacggtca ttctgatttg gccatggata tttcgcttca catgtttgat aaagtcaagg
      481 aagcacatga actggtcgca gggtctgagt atgagtttgg caggataata tatatggaaa
      541 aactacaaag catctacaat gatttgaaaa ggaatattcc tgtacatagg aaggccatta
      601 tcgaatactt tgaaaaatat ggatatgata tagagcttga tattgatatg gtgatggaca
      661 ttctctctcc catgaatgat aaaaaaccgg attacgataa ttttcagaag aataaaagcg
      721 gtttttcaga tttttcatac gctcttatga taaagttttg tcgatatatg ctggatacga
      781 agaatattag cttctgtacg tcaggtgaca tctggttcat agcacttcac agtttcaaag
      841 ccgatttacc cgctaaaaca cctaaaacga attttgataa tgtatttgaa cttgacgagg
      901 acaaatatga tgaagagata tcaggaagga tggggtttat atctaataaa tatacatgtg
      961 gttttgcggt tgcatggggc atgatatatg tgtatgactt tctgtataaa tatggatata
     1021 tttctgaaaa tgtgtactac gatgcccttg aagttattga cggtataaag gttgaaataa
     1081 tcaaaggata tgccaatagc ttatgggaat atgattttgt atatgcatgg aaaaagcctg
     1141 attcaatcag tgatgaggaa ttcattatag aaaagaacct attcgacgat tcatttaaag
     1201 tacaggtcaa aattgtagat gatttaactt tcaatgatat aattgaagat gataatagta
     1261 ctgaatagga gtagtaagaa atgtaatcgt caaatagaac atgttatatt ctaagtaaaa
     1321 cagagttcca cgttacgcag aactctatgt gctttaagta ttggatttct tggatttgca
     1381 gatttccggt atcttatctg accatggtgg aactcttgta gaaaatcctt actgctacct
     1441 gtgtaatgtt cacaggcagt tgaggatgaa aatcaattaa taaatgatga agtgaagttt
     1501 gttggtgtta accagattga ggtggaatta ggctaatggg tgaacaactt ccagttatca
     1561 caggcaaaga tttacttcag ttttatataa aaataggctg tgagctaaaa agagttaagg
     1621 gcaattgtgc aacaatcagg ccttgaaatt gttcatttta taagactatg gaagcataga
     1681 taagttctag aggagcgcca tctgtggcgc cctccgagtc acggggtaag gcctatcgcc
     1741 taacaaaaca ctcaagtaaa ggttgctggc ttatattgta aatgatggaa ttggatcagt
     1801 cagacatagg tagccagcac cacacgtact cagcaagata ggagctatct aagcttctgg
     1861 gcatgtcttg agtgcggatc cgttagctga aacaccctgt ttggggtaca gcttcgtaga
     1921 tataggttgc tcaagctaat aataatcgtg agggatgaaa gatgaagtat ttgattatca
     1981 acggtagttc tcgtaaaggt aatacgtgga aattagctga attaattaaa aatagtattg
     2041 agcaaatttc tccagaatca agctttgaag agatacaatt gggtgaacta gaactaccat
     2101 tttgcgtagg ttgtagcctt tgttttagaa aagggcacca atattgcccg cattacagca
     2161 ttatgaagac actaatagag aaaattgagg aatgtgatgg cttaatattt tctgtaacaa
     2221 ctttttatat gcaaccaacc gctatgacaa agaaccttat tgaccatttt tgtttcatgc
     2281 tccatagacc tcatttcttt gataaaaagg ctatcgtagt atcaactgtt ggagctgtag
     2341 gtggaaagca agcagtaaaa tatcttgcag gtacattaaa gggtatgggt tttaaccgat
     2401 gttatcgttt cccattgtct gtatatagtt ggaataatta taatccggat attaaagcaa
     2461 aaaccaaatg tagacagtta gcttttaagt ttcataaaga tgtttcttca agaaaaatgc
     2521 tcgcaccatc cttttcagta ttaataccat ataatctttt cagaggaatg agcatttggt
     2581 atgcaaaagg tacagaatat gaaacagaag atggtgtata ttgggttgaa ccacaaagag
     2641 caaaatatac ctatgacaaa tcaatctctg taccattatt taaaaaaatg tttgggtata
     2701 tattctatca cttaggaaag acaataggga aatttgctac agttacatac aaaaaatgag
     2761 tatcattttg cgtagctttg aaggacaggg tgcatcagct agcaaaattt tcacacttcg
     2821 ggcgtgctct agggttattc ctgcctacat caactcaccg ggcgacaagc gccgctcatg
     2881 ggaaagactc tgtagtccgg ttcgttgctg tcgcaaatgc cgaatacatc aacaagttga
     2941 tacgcagttc attaactctg ttaaaggaaa ggttactaca tctacttaag acattggaag
     3001 gatttgaaaa ggacaacgat tgttactgca tgatacattt cgattacaac gacggaaatt
     3061 attcgataga ttttgataca ggacaaataa ccgtatatga tttcgataat tcattgttcg
     3121 gctggtatat gtataacctc gcagaccttt ggggaaatgg acagcgggtg atagaagaac
     3181 cgatttatgg aatattgatt tatctttttt atagaaactt tggtctagat ttacataata
     3241 ttcctaaatg ttttgaagat tttaagctta ccttccactt tggcggattg tataaaatta
     3301 tttctgatga ataatgaact tccttttaaa atcgaaccac ttttttataa tatctaattt
     3361 gggaagtcga acagagcaaa catcaattaa aacacattat aagtattatc ttatgcatta
     3421 atgcccataa aaatgaatat gaaaacttct ttaagcatgg cggtgaagaa gccttacaat
     3481 acatgttgat ccagttcgag gctggtaatg cttaagaact gcgcgggcag attatgatgc
     3541 agctgtgcaa agagcttttg aaagtgaata tactaaatta actcaatatt tttgataatg
     3601 ataccccgaa aacactatgt attagtatat tgtgatatac ttataatgcg ataatgtaaa
     3661 taaagcctca ttgacgatta ttcccatttg cgggtgtatt atatggtgga aaatatcaaa
     3721 tttgattaca aaaaagagag tttggagcat gaaatttcat tagtttttga aaaacaatat
     3781 gggaaactat ttggcattgt ttatcgtatg acagagagtg cttaagatac tgaagatgta
     3841 ttgcagacag aatatcgggg gtgtgaatat gaaaacgaat aaagaccgcg ttcaatggct
     3901 ggtgctgatg ggcgtggcag ggacagtctt ttattttctt catgtaatat taggtgaaat
     3961 aaattacccg ggctatcatc ctttggagca ggcagtaagt gaccttaccg cggcaacagc
     4021 gccttcaagg gaaatagcat cggcttattc tacggtatat gcttttttca ccgttactgc
     4081 ctgtaccttg ctctgtgtat tttatcaagg aagggtaaat aggatttttc gtcttggaat
     4141 ttacttgttt actgttatgg aatggatttc cgctattggg tatacactat tctctttatc
     4201 aagcagcggt tatgccggaa cttttcaaga tgtaatgcat atggttgtaa ccgcttttgt
     4261 ggtattgctg tccattactt caatgatttt gatagcggtc ggttgtttca gaagcaaggt
     4321 tcataagcga ttcggcattt ttactattgc tgtgttggga atcatggcct tcggctccat
     4381 cgcaacgggc attgtgccgg ttgggtattt cggtattgcg gagcgcttaa gtgtgtattc
     4441 agttgtcgtt tacggcgcag tcctatcgct gcttactttt aagcattcaa tataaagcca
     4501 ataagccact aggaacgttt gacaagaggc aggcggtaga acccgaagtc cactaaagta
     4561 ttaataagga ctgatactct gtaaaaaaaa gcttttgatc tggttagtgc cgttaaggtt
     4621 tgcttctttg aaaattgaat cctctccact ttaaatataa catacaaaaa attgtaaatg
     4681 tagactgttt gttcttattt actactgcta taatgcaata atatgttaat gcaatttgtt
     4741 agagaggatt attatggaag ggatgtcatc aaaattatca aataagaaat cgacaaaaga
     4801 gggaaatgta taagttccta tgtgcttggt tttcaggata ttgacaaaac aaaactcatg
     4861 gttgttgggg gtaatgctac ccaactgata aaagatgggc aacgagttcg catgcataga
     4921 acagaagggt ataaaacagc ttatgacatc aatgttatct tcttaagcca tgttgatata
     4981 ctgatatatt tttttcagga tactttgcag cctttttgtc gtctaaagcg tatatagatt
     5041 aaaggaggtg aagcaaatgg catataagtc ctatcggccg gaggactttg aaacggttgt
     5101 gataaaacat gaaaacaggt tatatcgcac tgcactcgct attataggaa ctatgtccga
     5161 tgccgaggac atagtgcagg aggcattttt acgtgcttat gagaaagctc ccgagtttga
     5221 atcagaagaa catgaaaaag catggctcat aagggtaaca gtaaacatct gcaatagccg
     5281 attgcgatcc ccttggcgca agcgaacagt gcctctgctc gattcttatc cagcttcaga
     5341 gccggagcag catgagttac tggagcaaat catggcctta ccatctaaat atcgcacagt
     5401 catccatttg ttttattatg agggttattc cattaaggat atttctgggt tgactgggca
     5461 aaaggaatct acagtaagaa gtaatctgac tcgcgcccgt caaaagctca agtccgtttt
     5521 aaaggaggac gattatgaaa gcatataacg aatacatgaa taaaataaag gtatcaagca
     5581 cgttacatca aagactagta tcccgtaccg acagtgccag atccaagcgc tgctctatta
     5641 tgatcaagcg ctatgtcgca gcctttgctt gtcttgcact ggtcttgctt ggagttttta
     5701 caatgcctaa attaatacaa cacaatgtaa caccgatacc gggtgataat ccgagagttt
     5761 tgcagcccag cgcagacaca tctacacctg atgcatcaag caaatacgca ctggttttca
     5821 ataaagctga agaacagctc gcgaacgaca ttggaattcc aggtcatttc tggcaagagt
     5881 tgaccgccga cgagttaaat actgttttcc ctggtctgac agatacacat accgttacag
     5941 ctgccgcaaa ttttcagacc gatgaaaacg gagtagcctt attcaacatt gacgcccaca
     6001 tagtatccgc atctggattt aaaacatata tccagattgc gcctgacaag gtcatagttg
     6061 attataggct tgagggtgaa acgaaaacct cagatgtact cggaacagcg gtcacagcag
     6121 gctattttga gacaaaacct aatagcaagg ggtttaggaa tgtcatttat tttgcggctt
     6181 tcaagctttc agatgttgcc tattatgtgg aacttggcgg cgcagaagca gaaaaggaag
     6241 ctcttaagaa tgaaatctct gagctgattg gccttttgat tgaaggaggc gcagccgacc
     6301 ttgatgtatt tcatcctgtc gtaccagagc tgagagagga tcaactgagc cttgatgaag
     6361 cccgtgctga tgccgattac ggtgcgtatt tgccgacaac acttccagat gggttcgttt
     6421 ttgaggatgc gctgcgttat atcaatcaag agcataatgc actgcatgtt aactggacaa
     6481 aaggcatggg atatattaat tggcgtgtct cgttactgga tgataatgac aaaactcgta
     6541 tcacttctgt tgctgatacg aagaattatg accttacgct ctatcccatt ccccgcgccg
     6601 attctgtgcc tgacgaatta cgggagatcg tgaacgatcc tatctttctc gctgatgagc
     6661 taacgcttga agcagtacag gcacgaactt atgaggtctc agactcaggt gatgagctaa
     6721 gtccgcgaat gcgattcagc gtattatttg gagatatctt ggtggagctc agcataaaag
     6781 gtgcttcatc ggaagtaatg tttgatatct tgcagcagat cataaaataa caggaatttg
     6841 gtaagtaatt cataaaaagg gacaattctt ttgtttcgtt atatctgacg aaacaaaaga
     6901 attgtcccta tatactattg acagtatgga tggtgctttt tctctgagct tgccagggat
     6961 ttatatcaaa tccctgaaca agtcttcaac attcatggat aaaccctgga atacaaggga
     7021 tgtggcaaca tatccctttt cgaaaacttt aaatctgtca atttcataat ttgcaaagtt
     7081 gtataccaaa atgctttcct gtttagtatc aatgatccag tattccttaa cacctgataa
     7141 catgtatgtg tttaacttgt ctatcatatc cttgcttcgc gtgctgtccg aaagaatttc
     7201 gatagttagt gtcggggtgc ccatatatcg ctctttttct gtaacattgt tttcaaggtc
     7261 acaggctact aaaacatccg gctgcattac atcaggttct ttaatatctt tctttttaaa
     7321 gtgaacatca aacggagcca aaaatactct gcatttttta cttttgaaat attcactgaa
     7381 aattaagtac aatcttccca gtatctcctg atgtcctatg tttggagacc ccaacaaata
     7441 aatttcgcca ttgataagtt ccattctcag tgtgcttttt tccgtaattt ctataaactc
     7501 ttcataggaa actttttttc caccatactg ataatctaat gcgttttctc ttgctatgaa
     7561 atactgttct atatctgtaa catagggagt taatctggca gttttaatac cgttttttgt
     7621 aataaccaca tcattttgct gttcaataaa atccatgaat tttccaaaat tctgcttaaa
     7681 ctcggttgcg gtaactacaa ccccttctat ataattgatt gatttcacaa aaccacctcc
     7741 gggaacagca tatcataatg cacgataaaa tacaattaag tgtgcgaaac aattaaataa
     7801 tataaccgat taatttagtg tgatagcaca taagtaaaaa cagatgcacc tgagaacaag
     7861 aatgtactag tatcaattct tagcgatcct gagcatgaaa tacttcttta gactgataga
     7921 cctgtaataa ttattggtaa tatgtcaaat agcaaatgtg ctgcttatct atattacaca
     7981 ttttgtagaa atttgtatct tcttctgcta taattaataa ataaatgtaa aggaagcgaa
     8041 tactcatatg ctctctcgga actatgatat ggatatgt
//
LOCUS       DAOSDD010000205         8036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3318_length_8036_cov_6.012780, whole genome shotgun sequence.
ACCESSION   DAOSDD010000205 DAOSDD010000000
VERSION     DAOSDD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_8036_cov_6.012780"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1197)
                     /locus_tag="PK733_15155"
     CDS             complement(82..1197)
                     /locus_tag="PK733_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOJ11911.1"
                     /translation="MKSIILTGGGTSGHVTPNIALLPKLKELGYKIYYIGSMNGIEKQ
                     LIEKEGIPYYPINAGKLRRYIDAKNFTDLFRVIDGFRQALSIIKKLKPDVVFSKGGFV
                     SCPVVWAAWIRRVPVVIHESDMTPGLTNRLSMPFASKVCYTFPESEKNIPKGKGALTG
                     IPIRESILHGDKDIGRNICGFTDKKPVLMVIGGSQGSENINKAIRNSLNALLKDFQIC
                     HICGKGNVKHDFDNIIGYKQFEYVDKEQPHIFALADIVVSRAGATTLFEILALKKPNL
                     LIPLSRNASRGDQILNAESFQKQGFSYVLMEKSLSGETLISSVKETYKNRNKYIKDMN
                     KSDIGNAADFIVKIIQECSMYREIHDKLRNVDESKKL"
     gene            complement(1287..1877)
                     /locus_tag="PK733_15160"
     CDS             complement(1287..1877)
                     /locus_tag="PK733_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ11912.1"
                     /translation="MKRVAEQVVPNRADAYELLKEYNKNESLIKHALAVESVMLNFAE
                     LFGEDKVKWGVIGLVHDLDYEMYPDEHCKKTKEILKGKNWPEDYIRAVESHGWKICSD
                     VEPVDRMEKVLYTIDELTGLVAATALMRPSKSILDLEVKSVIKKWKQKGFAAGVNREI
                     IEEGAELLGIELGKVIEETIKGMQKVAESIGLKGNL"
     gene            complement(2090..3040)
                     /locus_tag="PK733_15165"
     CDS             complement(2090..3040)
                     /locus_tag="PK733_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ11913.1"
                     /translation="MTGGLWDTRAAPETQDEDFTNENLIKLVNMKDKESIIRNGGNEA
                     KMNIGIATRSFTGMTTRETAETMAEQGFKCTELCFTQTDCNYWKYNSTSDISELTNRR
                     VEDIVNMYRNSCIEVVSIGVFTNLIEPDDQIRDLNLNYFVKHMEYANYCGIKFVATEC
                     GFIQGNRGIQAHTYESAYNRILDSVKYLAEESEKHNVFIAMEPCVLDVIPSAKRMKDF
                     IKQSQSDRVKVLLDPANLIANSSEEDMFKYLKEDIAYFHGKDRKVNDAYGCVLGDGDI
                     DWPLFLSLYHQHTEGVPLILEYANSTNCGQIKQRVLDYDA"
     gene            complement(3098..4939)
                     /locus_tag="PK733_15170"
     CDS             complement(3098..4939)
                     /locus_tag="PK733_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11914.1"
                     /translation="MRHIIKIFGFRKLKLSHQLYLLISFSICVIMLVNGIFYTCLSYT
                     MIDRIKMYAQDTMNQLEFNLSVIIEDIKRSGFAIGYNKYVQEYLVADTIKKRLEARDY
                     IMDAVGYIADKSEYISALVLYNTEGIISILKDDPDIRYYIKLLEKNYSFERNDFVVPI
                     FSNFMTKDDSVREKKYYFAYLVPIYYSGEDREHMGWIGTAVVICDVGKLNNLIEQLKI
                     SKNTIINLYDQNNNVIISNNSDSESDRNNSKNNDSKTNSNKELESGKYIYTTRIIEDI
                     QCTIECKTPSNEVRDDISFFKDNLLLTAVIMVILLILIGAIINISITSPIVKIVNAIR
                     EIGEKNLKQRINLKLDGDIGFIAQNINIMLDKVEQMARRVFTTQDKLYQMELLKKQAE
                     LSALQNQINPHFLYNTLECMRSIGIAFNIDEIVEISTAMADIFRYSIKSDDVVDVKAE
                     IEIIGKYFKIIKIRFLGRFNVKVDVDDEIMNCRIPKMILQPIVENAVYHGLEKKDGTG
                     TLSIYGKIKNEEIVFIIEDDGVGIQPDELERLSDAIDEHSNEQNYVKDKRSIGLANIN
                     SRIKLIFGKDYGLKIESLPNVGTKVTVSFPATSLEGRRNTELEHLIN"
     gene            complement(5193..5948)
                     /locus_tag="PK733_15175"
     CDS             complement(5193..5948)
                     /locus_tag="PK733_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11915.1"
                     /translation="MYKVVLVEDEPWTLLGISKTFKWNEMGFKVINQTTRAMDAIDII
                     LDNNPDVVFTDIRMPQISGIELMRKIRNEDRDTEFVIISGFAEFEYARDAITLGAFEY
                     CLKPISKDAADSILERLKEHLDKKRNITNVNSVNKIMDVENKIHNVNFKNLLNYINEN
                     FKKNLQLKDLADQFYLNPNYCCYLFKNLTGNSFSEYLTSKRMGRACKLLKDTTLTISE
                     VAAEAGYNDYYYFNKTFKRQFSCTPSQYRKDWN"
     gene            complement(6333..7964)
                     /locus_tag="PK733_15180"
     CDS             complement(6333..7964)
                     /locus_tag="PK733_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11916.1"
                     /translation="MRSKQKLTHIIALILVICFVVTMFSACTVKQGSDTKGEEKTTEA
                     GVEIDDGSKTYVYNMNIHNAQNEWLKCEVLKTWREKFNIEFNFLPITWNDWQQKVMIW
                     MSSGDVPDVTYWDMKLSMANDYEKWVKAGLIKEIKGIDKYPNVKKHMDAMKVDDALMV
                     DGKKYVWPCSKDTTLVGDISCRGFIYRKDWARQLGLVKEDDIYTWEEMVEFAKQCVEK
                     DPGGVGKNKVAGLACTTWAWPNSLGVEQFTAGVIGSGGYTKKDGKWEWNARAPEYLEA
                     IKEIKRLYDAGIIWVDNALCKGDEPSQKFQAGEIAVYYDNIFAYNLHDKRIKMTENIQ
                     GLKPEDITYMKVKTPYGKFAAIRDTEFWCVSLFSAKMSDAKFDRLMKAWDWMLTDEGY
                     FTRQIGIRGIDWEFEGDSVKYLWPYNEETKRYERPKDNPTYIGQLPYQPIGITEGFGF
                     LDPNIKSTNAGKDAYDYCKWCEKNAALIAPNYALSTFKAPNKDKYGSFANDMKDAVIQ
                     IIISSTDPDKDWNDFLKSKEDKVNLVIDELNAGLK"
BASE COUNT         2302 a         1664 c         1101 g         2969 t
ORIGIN      
        1 gctttaccag atgggattcg cacccactat atattaatca ccgaacaggc gcacgtttca
       61 ttttcaccac tcctcgctaa attataattt ttttgattca tcaacattcc ttaatttatc
      121 atgtatttct ctatacatac tgcattcttg tattatttta actataaaat cagccgcatt
      181 gccgatatcg cttttattca tatcttttat gtatttattc ctatttttat atgtttcttt
      241 aacactgctt atcagggttt ctccagaaag ggatttttcc attagtacat aactgaaccc
      301 ttgtttctga aatgattcag catttaatat ctggtcccct cgactagcat ttctggacaa
      361 gggtattaac agattcggtt ttttcagagc aagtatctca aacagtgtag ttgcccccgc
      421 ccttgataca actatatcag caagagcaaa aatatggggc tgttctttat ctacatattc
      481 aaactgttta tatcctatga tgttatcgaa atcatgctta acattacctt tcccacatat
      541 gtggcatatt tggaaatctt ttaataatgc gtttagagaa tttctgatag ccttgtttat
      601 attctccgaa ccctggctgc ctcctataac catcaaaact ggctttttat cagtaaatcc
      661 gcaaatattc cttcctatat ctttatctcc atgcaaaata ctttccctaa tgggtatacc
      721 tgttagggct ccttttcctt taggtatgtt tttctctgac tcggggaaag tatagcatac
      781 tttacttgca aagggcatag ataacctgtt ggtcaggcca ggagtcatat cagactcatg
      841 tattacaaca ggcactctcc ttatccatgc agcccataca acagggcagg acacaaatcc
      901 gcctttgctg aatacaacat caggttttag tttcttaatg atggatagtg cttgtcgaaa
      961 gccatcaata accctgaata aatcagtaaa gtttttagca tcaatatatc ttcgtagttt
     1021 tcctgcatta ataggataat atggaatacc ctctttttca attaactgtt tctcaattcc
     1081 attcatggac cctatgtaat atatcttata tccaagttcc ttgagttttg gtagtaaggc
     1141 aatgtttggt gtaacatggc ctgaagtgcc cccacctgtt aatataatag atttcaatta
     1201 aatcacctct ttaggtctta agtcttatga ctaactgctt tttatcaact gcttttgact
     1261 aactgctttt tatcgactgc tttttattaa aggttgcctt ttaagcctat gctttctgca
     1321 accttctgca tacccttgat ggtttcttca attactttac ctagctcaat ccctaagagt
     1381 tctgctcctt cttcaataat ttccctgtta actcctgccg cgaatccttt ctgtttccat
     1441 ttttttatta ctgatttaac ctccaaatca agtatgcttt tgctcggcct cattagtgca
     1501 gtagcagcaa ctaatcctgt taattcatcg attgtatata aaactttttc cattctatca
     1561 actggttcaa catcagaaca gattttccat ccatggcttt ctactgcccg aatataatct
     1621 tcaggccagt ttttcccttt taatatttct tttgtttttt tacaatgctc atctggatac
     1681 atctcgtagt ctagatcatg cactaagcca ataactcccc attttacttt atcttcccca
     1741 aaaagctctg caaagttcaa catgaccgat tcaacagcca gtgcatgttt tattagactt
     1801 tcatttttat tgtattcttt aagaagttca taagcatctg ctctatttgg tacaacttgt
     1861 tccgcaactc gtttcatagt gaaatccttc cttcgttcat taataatact gtcttcaaca
     1921 ggtttctatt atataaataa tgctgttttt aataattttc ctttataata gtaatattat
     1981 tttcgatgag ttactcctct atttataaat tttagatacc tccacaaaga atgtcaagtt
     2041 ctaaaataat catttagcac atttatttag tatgatgcaa gtatgctcct tatgcatcat
     2101 aatcaagcac cctttgcttg atttgaccac aatttgtact atttgcatat tccagaatta
     2161 atggtactcc ttcagtatgc tggtgataaa gtgaaagaaa cagcggccag tcaatatctc
     2221 catcacctaa aacacatcca taagcatcat tcacctttct gtccttgcca tggaaataag
     2281 caatgtcttc ctttaaatat ttaaacatat cctcttctga tgaatttgcg attaaattcg
     2341 cagggtcaag caaaactttt actctatctg attggctttg tttaataaaa tccttcatcc
     2401 tttttgcgct gggaattacg tccaaaacac atggctccat tgcaataaat acattgtgtt
     2461 tttccgattc ttctgcaaga tattttacac tatcaagaat tctattatac gcagattcat
     2521 aggtatgtgc ctgtataccg cggtttccct ggataaatcc gcattctgtt gcaacaaatt
     2581 taattccgca gtaatttgca tattccatat gcttaacaaa gtaatttaga tttaaatctc
     2641 tgatttggtc atcaggctca ataagatttg taaaaacccc tattgacaca acttcaatac
     2701 aactattccg atacatatta acaatatcct caacacgccg attggttaat tctgaaatat
     2761 cggatgtact gttatatttc caataattgc agtcagtttg agtaaaacag agttctgtac
     2821 atttaaatcc ttgctccgcc atagtttcag ccgtttcacg ggttgtcata ccggtaaaac
     2881 ttctagttgc gataccaata ttcattttag cttcattccc tccattcctt ataattgatt
     2941 ccttatcttt catgtttaca agcttaatta agttttcatt tgtaaaatct tcatcctgtg
     3001 tttcaggggc ggctcttgta tcccacaggc cccctgtcaa gacttaatct taatggagaa
     3061 ccgtccccga aaattctggc acaagttcaa cgcaggttca attaattaag tgttctaatt
     3121 cggtatttct gcgtccttcc aaagaagttg ccggaaagct gacagtaact tttgtgccaa
     3181 cgtttggcag actttctatt tttagtccat aatcctttcc aaatataagt tttattctag
     3241 aattaatatt tgcaagccct atgcttcttt tatccttaac ataattttgt tcattactgt
     3301 gctcatcaat tgcatcactt aacctttcaa gttcatcggg ttgtatgccc acaccatcat
     3361 cttctataat aaacacaatc tcctcatttt tgattttgcc atatatgcta agagtacctg
     3421 taccgtcttt cttttcaagg ccatggtata ccgcattctc aacaattggt tgcaaaatca
     3481 tttttggtat cctgcagttc attatttcat catcaacatc gacttttaca ttaaaacgtc
     3541 ccaagaaacg tattttaata attttgaaat actttcctat tatctctatc tcagctttaa
     3601 catcaacaac atcatcactc tttatgctat atctgaaaat atccgccatg gcagttgaga
     3661 tttcaactat ctcgtcaata ttaaaagcga ttcctatact tctcatacat tccagggtat
     3721 tatagagaaa atgtggattt atctgatttt gaagtgcaga cagttctgcc tgtttcttta
     3781 aaagttccat ctggtacaat ttgtcctgtg tagtaaatac ccgtctagcc atttgctcta
     3841 ccttatccag cattatgtta atgttttgag ctataaagcc gatatcacca tcaagtttaa
     3901 gattaattct ctgttttaga tttttctctc caatttctct tatggcattt actattttta
     3961 ctattggaga agtaatgctg atatttataa tagctccaat taatataagc aggattacca
     4021 taataactgc agttaataaa aggttgtcct taaaaaatga tatatcatct cttacttcat
     4081 ttgatggtgt tttacactca atggtacact gaatgtcttc aataatacga gtggtataga
     4141 tgtattttcc tgattccaat tctttattac tattagtctt gctatcatta ttcttgctat
     4201 tattcctgtc gctttctgaa tcactgttgt ttgaaattat tacattatta ttttgatcat
     4261 acagattaat tattgtattt tttgagattt taagctgttc aattaaatta ttaagttttc
     4321 ctacatcgca aattactacg gcagttccaa tccaacccat gtgttcacgg tcttctcctg
     4381 aataatatat aggaacaaga tatgcaaaat aatacttttt ttcccgaact gagtcgtcct
     4441 tagtcataaa attagaaaat ataggtacta caaaatcatt tctctcaaaa ctataatttt
     4501 tttcaagaag cttaatataa tatcttatat ccgggtcatc cttcaggata ctaattattc
     4561 cttccgtatt atataatact agtgcggaga tgtattcact tttatctgca atataaccta
     4621 cagcatccat tatgtagtct ctcgcttcaa gccttttttt aatggtatcg gcaacaagat
     4681 attcctgtac atatttatta taaccaattg caaatcctga tcttttaata tcttcaatta
     4741 ttacactcag attgaattcc agttgattca tagtatcttg tgcatacatt tttatccggt
     4801 caatcattgt ataactaaga caagtataaa atatcccgtt taccagcatt attacacaaa
     4861 ttgaaaagct aattagtaaa tacagctgat gactgagctt aagttttcta aaaccaaata
     4921 tctttattat atgtcgcatc gcacatcata gtcccctctc actcataaat atatataata
     4981 caaaaatgat tctgcaagga acaaattaga tttttgcata cctccattta ggtgcaagtg
     5041 tgcaccttta ttcactaagg taacataagc gcataaaaat tttatatgca ttttcttttg
     5101 tgcgttatca atagctgaaa atatttatgg tatattatta ggtaatcttc gtacggctaa
     5161 atgattttaa gcaagcagtg ttagtatatt atttaattcc aatcttttct atactgcgaa
     5221 ggtgtacagg aaaactgcct tttaaaagtt ttattaaaat agtaatagtc attatagccg
     5281 gcctccgctg cgacttccga gatggttaga gttgtatctt ttaaaagttt gcaagccctt
     5341 cccatcctct ttgatgtaag atattcagaa aaagagtttc ctgtaagatt tttaaacaaa
     5401 taacagcagt aattagggtt aaggtaaaac tgatcagcaa ggtctttcaa ttgcagattt
     5461 tttttaaaat tttcatttat ataattaaga agatttttaa aatttacatt atgaatttta
     5521 ttctccacat ccataatctt attcactgaa ttaacgttag ttatatttct ttttttatca
     5581 agatgctcct tcaacctttc aagaatcgaa tcggcagcat ctttactaat aggtttaagg
     5641 caatattcaa aggcaccgag ggttatagca tcccttgcat attcgaattc tgcaaatcca
     5701 cttattatga caaattctgt atctctatct tcattacgga tttttctcat taattctatt
     5761 ccggaaatct gaggcattct aatatcggta aatacgacat cagggttatt atcaagaata
     5821 atatcaattg catccatagc tctggttgtt tgattaataa ccttaaaccc catctcattc
     5881 cacttgaatg ttttcgaaat tcccagaagt gtccatggct cgtcttcaac aagtactact
     5941 ttatacattc atattcaccc catatttata actatctgat gattatctta attatcttat
     6001 caccatgaaa atttgcttta tttatggtat cgaagtaaat tttcttatac tcgggtaaac
     6061 ataccatagt acaaaatgtt tggaaaatat ttttagtctt cttatacaat taatcaatta
     6121 ttttataaac ataattttat catacattat taaaaatatt aatactattt taaatatttt
     6181 tgaatttgta tattatattc aagaactgtc cccacttatt ctactcactt attccattta
     6241 ttctattttt tattaaaaca agctgccctg gaaaaatatc aaggcagcct gttttagttt
     6301 tgttagctat ttaattccac tagtttaccc cactatttaa gtcctgcatt gagctcatca
     6361 attaccaggt taaccttgtc ctctttcgat ttcaaaaagt cattccaatc cttatctgga
     6421 tccgtggaac ttatgattat ttgaataaca gcatctttca tgtcatttgc aaaacttcca
     6481 tacttatctt tgttaggtgc tttgaaggtt gaaagcgcat agtttggagc tattaaagca
     6541 gcattttttt cacaccattt acaataatca tatgcatctt tgcccgcatt ggtagattta
     6601 atattggggt caaggaatcc aaacccttca gttatcccta ttggttgata aggaagttgc
     6661 ccaatatatg tagggttatc cttgggtcgt tcatatctct ttgtctcctc attgtaaggc
     6721 cacaggtact tgacactatc tccttcaaat tcccaatcta tacctcttat acctatttgt
     6781 cttgtgaaat aaccttcatc cgtgagcatc caatcccaag ctttcatcaa tctgtcgaat
     6841 tttgcatctg acattttggc actaaaaaga cttacgcacc aaaattcagt atctcttatg
     6901 gctgcaaatt taccataagg tgtcttgact ttcatgtagg taatatcttc gggcttaaga
     6961 ccttgtatat tctcggtcat tttaattctc ttatcatgta aattataggc aaatatgtta
     7021 tcataatata ctgctatttc acctgcctgg aatttctgcg acggttcatc gcctttacaa
     7081 agggcattgt ctacccatat gatgcctgca tcataaagtc tctttatctc ctttatagct
     7141 tccagatatt ccggtgctct tgcattccat tcccatttac catccttttt tgtatacccg
     7201 cctgatccaa ttaccccggc ggtaaactgt tctaccccaa gagaattggg ccatgcccag
     7261 gtagtgcatg ccaagcctgc aaccttgttc tttcctactc ctcccggatc tttttctaca
     7321 cactgttttg caaattctac catctcttcc catgtgtaaa tatcatcttc tttgacaagg
     7381 cccagttgtc ttgcccaatc ttttctgtag atgaaacctc tgcaggatat atcaccaaca
     7441 agagtagtat ccttgctgca aggccataca tactttttgc catcaaccat taaggcatca
     7501 tcaactttca tggcatccat gtgtttctta acgttcgggt atttatctat accctttatc
     7561 tccttaataa gacctgcttt tacccatttt tcataatcat ttgccataga aagcttcata
     7621 tcccaataag taacatccgg tacatcacct gaagacatcc aaatcataac cttttgctgc
     7681 cagtcattcc atgtgatggg cagaaagtta aactcaatgt tgaatttctc cctccatgtc
     7741 tttagaacct cacatttaag ccattcattt tgcgcattat gtatgttcat gttgtaaaca
     7801 taagttttgc taccgtcgtc aatctctaca cctgcttctg tcgtcttttc ttcgccttta
     7861 gtgtcactgc cttgttttac tgtacatgct gagaacattg taacaacaaa gcaaattacc
     7921 agtattaatg caataatgtg tgtaagtttt tgttttgacc tcataataag atcctcctat
     7981 ttaaaactcg tggtcagggg cttagaagcc ccaaactacg gtatattttc ctttcg
//
LOCUS       DAOSDD010000206         8036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3319_length_8036_cov_5.298709, whole genome shotgun sequence.
ACCESSION   DAOSDD010000206 DAOSDD010000000
VERSION     DAOSDD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3319_length_8036_cov_5.298709"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..907)
                     /locus_tag="PK733_15185"
     CDS             complement(191..907)
                     /locus_tag="PK733_15185"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11917.1"
                     /translation="MKKLAFLMAVILSIMMVFTGCSKNNGSTSMKDQDTPTQTQDGAI
                     TDKLTEKPNVRVWIKKSFSAEADNALIERLEDYGKKTGTCDVTVEMIPNANFGEKYSV
                     AVESGEVPDVAYMTLYLLRQYYDADLLLDTEDLLSEIESSGHKLSTQSKAAATFNGKV
                     YAIPYYLSSTALLYRKDYLKAAGWDKAPETWEKLRQCAKDVTEKVPNVYGIGFTYLLR
                     HLRAHYFPRELPLRCYLYHL"
     gene            complement(1267..3102)
                     /locus_tag="PK733_15190"
     CDS             complement(1267..3102)
                     /locus_tag="PK733_15190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ11918.1"
                     /translation="MRNCFKVKIKVKEVVKGIGITWKLVFSNMLLIILSICFLSVVAV
                     KVSRNILVSQVSQNNLEAISQVNKSVNLILDNMVERLNTLYVDENFMNKFRKYCDMDR
                     EEYEKLLIQSSTMLNDYYFLMDKLAATAFSAKIEGYYMFVVTQEAIPYASWGRCEQDL
                     TEFTKECSKLFYDNHNKIMWLTTHRTIADSMYYPDDTKTFSAIKRISDHSQLKTLGYM
                     VLSISERNLYNTYENLIQKKEGSTLYIIDEQGNQVSGMCEEEKDRQLYRQIYEELSSQ
                     TQKNNKGYFFGVVDGQQHLITYNTIYRNNWNIVYITPFEELMSPIKFLQAAIILAILV
                     AFAIAFIMSFFISHSISKRLSSLRNVMLKQGENGPMTHARVDYNDEIGDLILCYNQMA
                     DRINNYSDMLLEEQKKKREMELSFLQMQINSHFLYNTLNSVKILARLNMNDEIGYLIT
                     SLIKLLKLTSSKTDLITIEQEIENVRHYVYIQKIRYLDRFEIEYNYEPWVANYQIPKL
                     LMQPVIENAIFHGISDMEGKGIITVKVERDGNDIYIRISDNGKGISEDVLQNILTGYQ
                     KMNSIGLHNIDERVKIYFGQEYGVSVETEIGKGTMVAIKIRALPV"
     gene            complement(3126..4691)
                     /locus_tag="PK733_15195"
     CDS             complement(3126..4691)
                     /locus_tag="PK733_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11919.1"
                     /translation="MIKVLMVDDERLIIQGFLHSVDWKSYGYDVIGTAESAKSALKIC
                     EKNLPDIAILDINMSGMNGLELGGKLKMLNPDIVIIILTAYNEFDFAREAVRLQFDEY
                     LLKPEIDFEDILETMERFSPKILIKRKNQMMKTSEGVWKLLCQLNEKEYLSGQQVQNM
                     FSELEEQRIYEQYACCYIHVSNSCIDELSLELFEAKGFDLLIDFMNYEGILFRPSSYS
                     YAVLVESQNDTQSLLLAENIVAMFDEENIAVTVGVSKCFSEVSKFHIAFYQAKNALMR
                     KFHINEQVLLYISKQDEKAVSTKDIKEYLIAGEKLLLMGDFNRFYTLTGQLLDSMSIT
                     QVSRGLLTSALIQALLLMTKKIESEGGNVNELLGKQEFNMFDEIQNYHSFTEMERWFR
                     SIFNLLMEYLFSNVNTPKSNIINDVLKYVSSNYENPELSLKNTAEHFYINYAYLSQLF
                     SREMSESFNHYLTRIRMEKAKKLVLESHVKLTDVALRCGYNNTSYFIKVFKRTTGMTP
                     YKYRISLKERRQI"
     gene            complement(5045..6406)
                     /locus_tag="PK733_15200"
     CDS             complement(5045..6406)
                     /locus_tag="PK733_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ11920.1"
                     /translation="MCFAKYGTEQLMTANESSITQDLYKHAKTIIPGGVQLLSKRPEM
                     LAPDVWPAYFREARGCEVWDMDNRHYYDMSTNGIGSCLLGYRDSDVTRAVMRRINLGS
                     MSTLNPPEEVYLADKLLAIHPWAEQVRFARTGGEIAAVAVRIARATTDRSIVAISGYH
                     GWQDWYLAANLGENDALRGHLLPGLEPLGVPGELRNTAVTFKHGNWEEFQKVLDQHGN
                     KLAAVIMEPCRYKDPESGFLEFVRSETEKRGILLIFDEITIGWRLNFGGAHLRFKVNP
                     DIAIFAKALGNGHPMAAAIGTKDAMQGAHSSFISSTYWTESVGPAAALATIDKLEKYD
                     VAGHVGVIGRRIKDLWKQYGCKHGLPVVVEEGYPCLAHFKFDHQLSQQLNTLYVQLML
                     ERGFLAGVSIYPTFAHTEEIVALYGEAIDEVFSIIAAYLKEGVVEKMLKGPVAHTGFG
                     RLL"
     gene            complement(6494..7903)
                     /locus_tag="PK733_15205"
     CDS             complement(6494..7903)
                     /locus_tag="PK733_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11921.1"
                     /translation="MDRKYLMYDRKWIANYHGKLLAKGSCSFNVCIPKKGTPFIKADK
                     PWESMSTGWGTMLYEDGLYRMWYEAWDSKYKNDHDGFLCYAESKDGENWIKPELGIYE
                     YEGSTKNNIVYNSKLAGNVGFHGHTIFIDPTSPENARYRMVHQLATKTWHEPYSRAFY
                     LMGLAYSADGINWKQGVPEYKCFIRRPCAPFGSDTQSVVYYDRKLRKYVGYFRNWEYD
                     YKRSIARSETTDLTDWPVPKTIIEIDELDKYGVDFYNTAASKYESGSDEAHFMFISMY
                     DHVDDTLNVEMAFSRDGIRFKRDDRTIIAGNDTPYDMGGIYVCPGIFNVGDKCLVAYH
                     GVDYKHEEALPNLISYAGNFNFLTYEKDRLRYMNIKDYFEFNLDDFICERQKIDILIN
                     ADTGTDGSIYAAIYNINPTEVIEGFSLEDCIPVVGNGLNMKLDFKNGNIINNKYLGKK
                     IGIRFYAKKCKIYSIEIKQ"
BASE COUNT         2403 a         1700 c         1167 g         2766 t
ORIGIN      
        1 aatatatttc ttttgtagtt ttgagcgaag tgttaatcat ccagtaaatc gggaacaggg
       61 taacaataag gaatattgct atactcaaat aaagaaacag tttttttagt ttgtcagatt
      121 ttttgccagt aatcattttg atcctcctat tcctccagta gttttctggt tactttattg
      181 attaacacaa tcataaatgg taaagataac accgaagagg caattccctt ggaaagtaat
      241 gagctcttaa atgccgtaat aaataggtaa aacctatacc atagacattg ggaacttttt
      301 cagttacatc tttggcacac tgtcttaatt tctcccatgt ttcaggagcc ttgtcccaac
      361 ctgctgcctt gaggtagtcc ttacgataaa gaagtgcagt actgctaaga taataaggga
      421 ttgcataaac tttgccatta aaagttgctg ctgcttttga ttgagtagaa agcttgtggc
      481 cactggactc aatttcagac aggagatctt cagtatccag caaaagatct gcatcataat
      541 actgtctgag cagataaagc gtcatatatg ctacatctgg aacctcaccg gattcaacag
      601 ctacgctgta tttctcacca aagttagcat ttggaatcat ttctacagtg acatcacatg
      661 ttccagtctt cttaccataa tcttcaaggc gttcaatcaa tgcattgtca gcttcggcac
      721 taaatgattt ttttatccag acacgtacat tcggcttttc agttagttta tcagtgattg
      781 caccatcttg cgtctgagta ggagtgtctt gatctttcat tgatgttgat ccattattct
      841 tggaacaccc ggtaaatacc atcataatcg atagtattac tgccattaag aatgccagtt
      901 tcttcataat atactccccc ttaattgact ttgttttgat taaattatta ctacagctgt
      961 aagaagtact tctttacatt cataattgta tacgatactt ataaaaaaca aaatcaaata
     1021 attttatgtt ctgcagcaaa tattttagct tttttattgt ttttttcttc cacaaataaa
     1081 tggcatagtt aatgcaaaat atttaatata aaaatacaac attctttaga ttgctttttt
     1141 aacccaagca tcaagaagtc tctcgcttct acaagcggga gttgaactga tgcaaattaa
     1201 aataacaacc ttgttcagtg ggggattaca agacccccac tgaatccccg agcgaagcga
     1261 gtttgcttac actggaagag cacgaatttt aattgctacc atggtgccct ttccaatttc
     1321 agtttctaca ctgacaccgt actcctgccc aaaataaatt tttacccgct catctatatt
     1381 atgaagacca atactattca tcttctgata cccggtcaaa atgttttgca gtacatcttc
     1441 cgagattcct tttccattgt cacttatacg tatatagata tcatttccat ccctttccac
     1501 tttgactgta atgatacctt ttccttccat atctgaaatt ccatgaaaaa tcgcattttc
     1561 tattaccggc tgcatcaaaa gttttgggat ctgataattt gcgacccatg gctcataatt
     1621 gtattcgatt tcaaaacggt caagataacg gattttctga atatagacat agtgtctgac
     1681 attttcaatc tcctgttcta ttgttattaa atctgtttta gaggaagtga gttttaaaag
     1741 cttaatcaac gaagttataa ggtagccaat ttcatcattc atattaagcc gcgccaatat
     1801 tttaacagaa ttaagcgtat tatataggaa atgagaatta atttgcatct gtaaaaagga
     1861 cagttccatt tcgcgtttct ttttttgttc ctccagaagc atatctgaat aattattaat
     1921 cctgtcagcc atttggttgt aacacaatat aagatcaccg atttcatcat tgtaatcaac
     1981 cctcgcatgc gtcataggtc cattttcacc ctgttttagc atcacgtttc tcaaactgct
     2041 caaacgtttt gaaatagaat gagatatgaa aaatgacatt ataaatgcta tagcaaaagc
     2101 tactaaaatc gcaagtatga tggcagcttg caaaaatttt ataggagaca taagctcctc
     2161 aaatggcgtt atatagacaa tattccagtt attccgataa attgtgttat atgtaattaa
     2221 atgctgctga ccgtcaacta caccgaaaaa atatccttta ttgttttttt gggtttgact
     2281 ggagagttct tcataaatct gacgatataa ctgcctgtct ttttcttcct cacacatacc
     2341 tgaaacttga tttccctgct catcaataat atacagcgtt gacccttctt ttttttgaat
     2401 aaggttttca taagtattat aaagatttct ttcactaata ctgagcacca tataacccag
     2461 tgtttttaac tggctatggt ctgaaattct ttttatcgct gaaaatgtct ttgtatcatc
     2521 aggataatac atggaatctg ctattgtgcg atgagtcgtc aaccacataa tcttattatg
     2581 gttatcatag aataatttac tacattcttt tgtaaattca gtcagatcct gttcacaacg
     2641 tccccatgat gcgtaaggaa ttgcttcttg cgttacaaca aacatatagt atccttcaat
     2701 ctttgcagaa aatgccgttg ctgcaagctt gtccataaga aaatagtagt cgtttaacat
     2761 ggtagaactt tgaattaaca atttctcata ttcttctctg tccatgtcgc aatatttacg
     2821 aaatttattc ataaaatttt catctacata taatgtgttt aatctctcaa ccatattatc
     2881 caaaattaga ttcacacttt tattcacctg cgatatggct tccagattat tctggctgac
     2941 ttgacttacc aatatgtttc tagatacctt tacagccacc acagataaaa agcaaattga
     3001 taatataata agcaacatat tactgaacac cagtttccaa gtaatgccta ttccttttac
     3061 aacttctttt acttttattt ttactttaaa gcagtttctc atagatattc accactcaat
     3121 gattattaaa tctgtcttct ttctttcaaa ctaatccgat atttatatgg agtcatacct
     3181 gtagtacgtt taaaaacttt aataaaatag cttgtattat tatagccgca ccgtaaagct
     3241 acatccgtga gttttacatg actttccaga accagctttt ttgctttttc cattctgatt
     3301 cgcgttagat agtggttaaa actttctgac atttccctac taaacagctg ggataagtat
     3361 gcataattta tataaaaatg ttccgctgta tttttaaggg aaagctccgg attttcataa
     3421 ttacttgata catacttcaa tacgtcattg atgatgttgc tttttggagt attaacattg
     3481 gagaacaagt actccatgag gaggttaaat atgctcctga accatctttc catttccgta
     3541 aaagaatgat aattctgtat ttcatcaaac atattaaatt cctgctttcc aagtaactca
     3601 tttacatttc ccccttctga ttctatcttc tttgtcatta acagaagtgc ttgtattaat
     3661 gcgcttgtta ataatcctct cgaaacctga gtaatggaca tgctatctaa aagctgtcca
     3721 gtcaatgtat aaaacctatt aaaatctccc attaagagta gcttttcccc tgctatcaga
     3781 tattccttaa tatcctttgt actaactgct ttttcatcct gcttgcttat atatagcagc
     3841 acctgctcgt taatatggaa cttacgcatt agtgcatttt ttgcctggta gaatgctata
     3901 tgaaattttg atacttcact aaagcattta cttacaccaa ctgtaactgc tatattttcc
     3961 tcatcaaaca tagctactat attttccgcc aacaataatg attgagtatc attctgactt
     4021 tctaccaata cagcatagct atagctggaa ggccgaaaca aaatcccctc ataattcata
     4081 aagtctatca acagatcaaa tccttttgct tcaaaaagct ccaatgacag ctcgtctatg
     4141 caggagttgc taacatgaat ataacaacaa gcatactgct cataaattct ctgttcttcc
     4201 agctcagaaa acatgttttg tacctgttgc ccgctcaaat actctttttc atttagttgg
     4261 cacaacagtt tccaaacacc ttcactagtt ttcatcatct ggtttttcct ttttattaga
     4321 attttgggag aaaaacgttc cattgtttcc aatatatctt cgaaatcgat ttccggtttt
     4381 agaaggtatt catcaaactg aagcctaact gcttcacgtg caaaatcaaa ctcgttgtaa
     4441 gcagttaaaa ttatgataac aatatcggga ttcagcattt tcagcttacc gccaagctca
     4501 agaccattca ttccggacat gtttatatca agaatcgcta tatctggtaa atttttctca
     4561 caaatcttta atgcagattt agcactctcc gcagtgccaa taacatcata tccatagctt
     4621 ttccaatcaa cagaatgaag aaatccttga atgatcagtc tttcatcgtc taccataagg
     4681 actttaatca tgtcagtata ccttctcttt ctatatgatt catatattaa tcataaaaat
     4741 ccagtttcag cgactggaac tcagtacaac taaagtcggc aggttcaaat attcgtaccc
     4801 gcgcctatat taataaatta gtcgcacaaa gtgtactgca tgtatttctt attaatatca
     4861 aaaagttaat aatagccact ccgtatttta ttaatataat accataaatt tgtacagata
     4921 aaaagcaaat ttatcaagcg cactttcttc ctatcttaaa gaaaagcttg ggaccccttg
     4981 aagttcaagg gttctcaagc tttttatatg ttgcgtcata tgttgctata atttttataa
     5041 tctactataa caatctcccg aaacctgtat gcgcaaccgg accctttaac atcttttcaa
     5101 caacaccttc ttttaagtag gcggcaatga tactgaatac ttcatctatt gcttcaccat
     5161 acaaagctac tatctcttcc gtatgagcaa atgtaggata aatactgact cccgcaagaa
     5221 atcccctttc aagcatcagt tgtacatata aggtgtttag ctgctgagaa agctggtggt
     5281 caaatttgaa atgggcaagg caaggataac cttcttctac tacaacaggc aaaccatgtt
     5341 tacatccata ttgcttccac aagtccttaa ttcttcttcc tattacaccc acatgtccgg
     5401 caacatcgta cttttcaagt ttatcaatag ttgccagggc tgctgccggg ccgacactct
     5461 ccgtccaata agtgctgctg ataaatgaac tatgcgctcc ctgcatagcg tcttttgttc
     5521 ctatagcagc agccatagga tgtccatttc ccaatgcttt cgcaaaaatt gcaatatcag
     5581 ggttgacttt gaaccttaaa tgggcaccac caaagttgag cctccaacct attgtaatct
     5641 catcaaatat tagcaaaata cccctttttt cggtttcact acgtacaaat tccagaaatc
     5701 cgctttcggg atctttatat ctgcagggtt ccatgataac cgcagcaagc ttgtttccat
     5761 gctgatccaa tactttctga aattcttccc aattaccatg tttgaaggta actgccgtat
     5821 tgcgaagctc acccggtacg cccaaaggct ccagcccggg taatagatgc cctcttaatg
     5881 catcattctc accaagattt gcagccagat accagtcctg ccaaccatgg taaccggata
     5941 tagctacaat ggaacgatct gtagtagctc ttgctattcg aactgctaca gctgctattt
     6001 ccccaccggt tctggcaaat ctcacttgct ccgcccacgg atgaattgca agaagcttat
     6061 ctgcaagata cacctcttca ggaggattca atgtgctcat acttcccagg ttaattcttc
     6121 tcataactgc tcttgttaca tcagaatccc tataacccag caggcaggag ccaattccat
     6181 ttgtagacat atcataataa tgcctgttat ccatgtccca gacctcacaa ccacgagctt
     6241 ctctgaaata ggcaggccat acatccggtg ccagcatttc aggacgtttg ctcaggagct
     6301 gcacccctcc gggaataatt gttttagcat gcttatataa atcctgcgtt attgaacttt
     6361 catttgcagt cattaattgc tccgtgccgt acttagcaaa acacatatcc tcaccactcc
     6421 ttttatcaat ggaaaactgg attttattac aaattttata tcctgcaaaa atatcttttc
     6481 attagctgat taactattgt ttgatttcta tgctatatat cttgcacttt tttgcataga
     6541 atcttattcc aattttttta cccaaatatt tattattaat tatattaccg tttttaaagt
     6601 caagcttcat attcaaaccg ttgccaacaa ccggtatgca gtcttccaat gaaaaccctt
     6661 ctataacttc tgtaggattt atattataaa tcgctgcata aatacttcca tcagtacctg
     6721 tatcagcgtt aatcaatata tctatttttt gtctttcaca aataaaatcg tctaaattga
     6781 attcaaaata gtctttaata ttcatatatc ttaatctatc tttttcatat gtgagaaaat
     6841 taaagttgcc tgcatatgat atcaaatttg gtaatgcttc ctcatgctta tagtctacac
     6901 catgatatgc tacaagacat ttatcaccaa cattgaatat accgggacat acatatatac
     6961 cacccatatc atagggagta tcattaccag ctattatagt cctatcatct cttttaaacc
     7021 ttataccatc tctgctgaaa gccatctcta cattaagagt atcatcaaca tggtcataca
     7081 ttgaaataaa catgaagtgt gcttcatctg aacctgattc atattttgaa gctgctgtat
     7141 tataaaaatc aactccatac ttatccaatt cgtcaatttc aattattgtt tttggaacag
     7201 gccagtcagt caagtcagta gtttcagatc ttgctattga tcttttgtaa tcatattccc
     7261 aatttctgaa atatcctaca tattttctta gctttctatc ataatatacc actgactggg
     7321 tatcgcttcc aaaaggtgca catggtctcc taataaaaca tttatattca ggtacacctt
     7381 gtttccagtt tatgccgtca gctgaataag caagtcccat taggtaaaag gctcttgaat
     7441 aaggttcatg ccaggtttta gttgccagtt gatgaaccat cctgtacctt gcattttccg
     7501 gtgaagttgg atctataaat atagtatgtc cgtggaaacc aacatttcct gctaatttgg
     7561 aattatatac tatattattt ttagtgctgc cttcatattc atatatccct aactcaggtt
     7621 ttatccaatt ttccccgtct ttactttcag cataacacag gaatccatca tggtcatttt
     7681 tgtatttgct gtcccatgct tcgtaccaca ttctatagag accgtcttca tatagcatgg
     7741 ttccccagcc tgttgacatt gattcccaag gtttgtccgc ctttataaaa ggtgtccctt
     7801 ttttaggtat acatacattg aatgaacagc tgcctttagc taatagcttt ccatgataat
     7861 tagctatcca ttttctatcg tacattaaat attttctgtc catgatttat atactctctc
     7921 ctgtctttta tttttatttc aaaacatata ctgcaaaaat tgatgcattt tcaatatata
     7981 atcttaattt gaaatttcct cttcccaatt ctctcaagct tctatctttc caggaa
//
LOCUS       DAOSDD010000207         8024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3331_length_8024_cov_7.793826, whole genome shotgun sequence.
ACCESSION   DAOSDD010000207 DAOSDD010000000
VERSION     DAOSDD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_8024_cov_7.793826"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3203
                     /locus_tag="PK733_15210"
     CDS             <1..>3203
                     /locus_tag="PK733_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11922.1"
                     /translation="QEYNGYYLSGSWDISSIEEEEWTIRATAYAEDGGTASETRRVFI
                     DHKGPPVPANFSAEVSSNNTISFTWDTVEGAEKYYLKREYANPEYIGSSQQTIYHPDH
                     SYEWSGLYTERPYIFTLRSVDTIGNQSEAASVIIEIHQGPEIVLDDGYNVYTKDSNYV
                     LKGTTVPGAVVKVNGESVSVDEGGKFSYNSVLTVIDNSFTIASEKDGVTHTVKQRVTF
                     DTKIPTISTFTPNNNAYFSGNKAQIKISTYDNTYIGRQVLQVKRSEELPWEDIFEIPY
                     GQTSPYFDTTVDIGDGGPLEDGSYKFRLLVWDRAGNVAGEDLVRVWNIDNTRPEPPTN
                     LTAEEEIGKIILSWDASTSSDLKTSSQYKLYRSTKAGGNYVLIGTTSNTRYENTTVSE
                     GVTYYYVVTAIDKAENESEYSNEISVSPENDITPPEFRSLPYDGRKYGGGEARISMEA
                     TDNSPKGVEKFILEYSNDNGASWKSLTLGASSKTVGEVKVYYRDYNMSTTGMVSGDYI
                     FRFSAVDYSGNTATTDRNLIFDLSVASIQNLTARSGEGSIILEWEVPPDNDISSYDIM
                     RSTYYSGGYSTIKTIYGGDTKTYTDTTASLDKTFYYKVVSKDSYYPYPNQSVSKIVSA
                     VARDDITPPQITYINIEDLAKIGGKTINLNVRATDNKKPIKVVFLGSQDGGATWEPLN
                     IKWINSTILPDNVTYSFTYNWTTTGFTSGEILVKIIVKDEAGNEGEAERTWNLDLYVS
                     PATNLRTTPGDGSILIEWDPVTDEDINSSNAYNVMRSENSGGPYSVIRYELGKNTTQY
                     TDTGVQTGKTYYYVIRSRDNFGNETLSLEIAAMPAGDEIPPVINAVTPDVGATIGGPV
                     NYVLRVEASDNAGMEGSTAEIEYSVDGENWTQVNQNIEGPYNLLNNKFYFTARWNLID
                     LVSGNYKIRYTITDGAGNSSNLVADYYVDRTGPSAPGNLLATYGQKAIGLVWEVVPET
                     DAASYYIYRANTLNGEYTRIKIIDIIDGEECNIYSDNTVMEGITYFYKVTSVDKFYQE
                     GPASNIASAAAISDTTPPVVTLIEPDHHTVLGLNAQIT"
     assembly_gap    3204..3303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3304..>8024
                     /locus_tag="PK733_15215"
     CDS             <3304..>8024
                     /locus_tag="PK733_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ11923.1"
                     /translation="PSVTGLKGTAFGGDIVLEWDSIPDRDLAYFLVEKKDRLGKYQEV
                     KKVYNILGLNISDLEPGVDYRFRVTAFDKMGNQGEPAEIIVNSGSDSTPPIVTYEYSS
                     HKIYSGNNSININPFIAKDNVGVKKLILEYSVDMINWINICERVIDKPARQVRFNENW
                     DVSSLAEGDYFVRALAEDEAGNMSLPSEAAIYTVDHTKPAAPFDLTFTATAGYIEVMW
                     KYLAEKDFSFFKLWRSESEDGTYENITPGYYVYMGYRDRTVETGKTYYYKVTVVDVAD
                     NESEPSETIIGSLTEDKIAPTIYFRNPLEGDILNSKALFSISFSDDLALKSMKVEYRK
                     EGSGDGDDSWEVIITRDYNPDGLSKKSYANEEFYWENKGLPEGRYKVRVTVEDSAGNI
                     TGPLVMTYLVNNVAPPKPTITAEAGGWQASLNWISDNNIEDFHMYKLYRTTVLGGSAR
                     NILSTGEKSFNDIGIQPGIRYYYYVRAYDIYGNYSQSDTVSVIPTAEDKILPVADAGD
                     EHILVVGMEGFFDGTLSSDNHRIASYHWDFGDGGTAVTSRPTHSYNAVGDYTATLTVT
                     DPAGNTAVDTTGVKVVSIDEVGILEVKVIDDVTGELLPGSSVVIDYPDGATHKTTTNS
                     VGAAFVIAPPGDYEVYAFKTDFRPASAKVTMVRGQKNTVMIRLPKGSVVTGELKAERM
                     DIDEIIAAGIDITDPANQNAYKFEIGLGFNMPDIPIIATKHKFIRPANAPQNYTVSED
                     GSKIIIRERIDEEGKGSGRIFFGEKIDASFDVPAMAYMVIPGKTTWLKEFFDIGLTIQ
                     NAAGPEFVIEDAWARLTIPDGLSLAPTREGQHEYVEMGSIPGGESKSVNWIIRGDKKG
                     EYRLKAEFNGLLMPFEDEIKQIFRNDEPFRVWAEDAVLMHIITQDRADKGYPYNLKLG
                     IENISEISLYNLSLELLEETKANYIYAPNQELIKSVAELKPGETLWGEYQLISAIEGL
                     LSLKQSYNLKTGGNTDIEEEMSAISVPENSPDNAPVLNQENKDDGTVNLSWEQVEGAL
                     GYKIYRIREDLRISMDPEELVYSCRLEGLPGELSEDISVNLPEPDGPKDYVINTLIAD
                     GEDVKEVTLHAITGFSWIENAAEPTITVEPETLIVGRETELLVTAKEGGKPVHIIANP
                     AERPSYRINGTIDIGDLAKNQRLNDNGQVRVVVKPEKPGPIEIKSYLLGRFITRKTIN
                     AVLPEEPKQPQGLKASKGDRKAKLTWFPNSEPEVKGYYIYRVIDNIWTKIHPGIYKCD
                     RSEGLVSYEVTGLNDNTTYTFQVSAVDAFNRESVPSKPAQVTLTIPEDTIAPWVVGSN
                     PVSRQKDVPVDTKIIQIFFSENIALWETYENIEVKAGDVTVEFIAEAEIGTNILTITL
                     NEELPLKTHCQVFIPEGAVRDKSFNELKSENKGDYAYKLEFFTEAENDTICPMLIAAS
                     PKENAQSVTVHSTIFLYFSEKIDEGPAYDNIMITANGIAVPHSKEINGAKIIIKPLSE
                     PTYKGVLPYNSKIEIFVPEGAVVDSAGNVLKNQHGLKFYTDTVPDLNAPKIIRMKPSA
                     NSRDLPLDTELNIYFSERIKEG"
BASE COUNT         2696 a         1371 c         1745 g         2112 t
ORIGIN      
        1 caggaataca atggatatta tttatccggt tcatgggata ttagctcaat agaagaagaa
       61 gaatggacta taagggcaac agcctatgct gaagatggag gaacggcttc agaaacccgc
      121 agagttttca tagatcacaa aggtccccct gtacctgcaa actttagtgc ggaggtttcc
      181 tctaacaata caatttcatt cacctgggat actgtagaag gtgcagaaaa atactatctt
      241 aaaagggaat atgccaatcc tgaatatatt ggttcctccc agcaaactat atatcatcct
      301 gatcatagtt acgaatggtc cggattatat acagaaaggc cttacatctt tacactaagg
      361 tcggtagaca cgattggtaa tcaaagtgaa gctgcatcag tcattattga gattcatcaa
      421 ggaccggaaa ttgttttaga tgatggatat aatgtttata caaaagacag caattatgtt
      481 ttaaaaggaa caacagttcc gggtgctgtg gttaaagtaa atggagaatc ggtttcggta
      541 gatgaaggcg gaaaattttc ttataacagt gtgcttacag tgatagataa cagttttact
      601 atagcttcag agaaggatgg tgttactcat actgtcaaac aaagggtgac atttgatacc
      661 aaaattccta ctatctccac cttcactcca aataataatg catattttag tggaaataag
      721 gcacaaatta aaatttcaac ctatgataat acttatattg gacgccaggt tttacaggtg
      781 aaaagaagcg aagaattacc ctgggaagat atttttgaaa taccttatgg ccaaacaagt
      841 ccttatttcg atactacagt agatattggc gatggaggcc ctttagagga tggctcatac
      901 aagttcaggc tgctggtttg ggatagagca ggaaatgttg ccggggaaga tctggtaaga
      961 gtatggaata tagacaacac caggccggaa cctcccacaa atctaacagc agaagaagag
     1021 ataggtaaaa taatattatc gtgggatgcc agcacatcat cggatcttaa aacatcttca
     1081 caatacaagc tttatcgctc gacaaaagcc ggaggcaact atgttctaat tggaactacc
     1141 tccaatacaa gatatgaaaa cacaactgta tctgaaggtg ttacatacta ttatgttgtt
     1201 accgcaattg ataaggcaga aaatgagagt gagtattcaa atgaaatttc ggtttcccct
     1261 gagaatgata tcacaccacc tgaatttaga agccttccct atgacggaag aaaatatgga
     1321 ggaggagaag ctagaatttc tatggaagct acagataatt ctcctaaagg agtagaaaaa
     1381 ttcatactgg agtattccaa tgataatggg gcctcgtgga aatcccttac tcttggagca
     1441 tcttcaaaaa cagttggtga agtaaaagtt tattatcgag attataatat gagtacaaca
     1501 ggtatggttt ccggagacta tatttttcgt ttttccgcag tagattattc aggaaacaca
     1561 gcaactacag atcggaattt gatttttgac ctttcagttg cttctataca aaacttgaca
     1621 gctcggtcag gagaaggatc aataattctt gaatgggaag ttcctccgga taatgatatt
     1681 agctcatatg acataatgcg ttctacatac tatagtgggg gttacagtac aatcaaaact
     1741 atctatggag gggatactaa aacatatact gatactacgg caagtttaga taagacattt
     1801 tattacaagg ttgtaagtaa agattcatat tatccatatc caaatcaatc tgttagcaaa
     1861 attgtatcgg ctgttgcaag ggatgacatt acaccaccgc agattactta tattaatata
     1921 gaagatttgg ctaaaattgg aggcaagaca ataaatttaa atgtacgtgc tactgataac
     1981 aaaaaaccaa taaaggttgt tttcttgggc tcacaggatg gaggtgctac atgggaacct
     2041 cttaacatta aatggatcaa cagcactatt ctgcctgata atgttacata cagttttacc
     2101 tataactgga caacaacagg ctttacttca ggggaaatac ttgtgaagat aatagttaaa
     2161 gatgaagcag gtaatgaagg ggaagctgaa agaacgtgga atctggatct ttatgtttct
     2221 ccggcgacaa atttacgaac tactcccggc gatggaagta ttctaataga atgggatcct
     2281 gtcacggatg aagacattaa cagcagcaat gcttataacg taatgagaag tgaaaattcc
     2341 ggcggaccat actcagttat aagatatgag ctggggaaaa acactaccca atatactgat
     2401 acaggagtgc agacgggaaa gacatattat tatgtaattc gttcaaggga taattttgga
     2461 aatgagaccc tgtctttgga aattgctgcc atgcctgcag gagacgagat acctccggtt
     2521 ataaatgctg ttacgccgga tgtgggtgct acaataggtg gacctgtaaa ttatgtgctt
     2581 agagttgaag caagtgataa tgccggtatg gaaggatcca cagctgaaat tgaatactca
     2641 gtcgatggag aaaactggac gcaagtaaat caaaacatag aaggacctta taatctatta
     2701 aacaataaat tttatttcac agccagatgg aatctaattg acctggtcag cggcaattac
     2761 aagattcgtt atacaattac cgatggtgcc gggaattcat caaacctggt agctgattat
     2821 tatgtagaca gaacaggacc ttcagcaccc ggaaatctcc tggcaaccta tgggcaaaaa
     2881 gctataggcc ttgtgtggga ggttgtacct gaaacagatg cagcaagtta ttatatttat
     2941 cgtgcgaata ctttaaatgg cgaatatacc agaattaaaa ttatagacat tattgatggg
     3001 gaagaatgta atatatattc agataacaca gtaatggaag ggattactta tttctacaag
     3061 gttacttcgg tagataaatt ttaccaggaa ggtcctgcct ccaacatagc ctcagcagca
     3121 gccatatccg atacaacacc tcctgtagtt acacttattg aacctgatca tcataccgta
     3181 ctcggcctta atgcacaaat aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnccttctg taaccgggct taaaggaacc gccttcggag gagacattgt acttgaatgg
     3361 gatagtatac ccgacaggga tctggcatat tttctggttg aaaagaagga tagattaggt
     3421 aagtaccagg aagtaaaaaa ggtatataat atattggggt taaatatctc cgacctggaa
     3481 ccgggagttg attaccggtt cagagttacc gcttttgata aaatgggaaa tcagggagaa
     3541 ccggcggaaa ttatagtgaa ctcaggttct gattcaactc ctcccatagt aacttatgaa
     3601 tactcttctc ataaaatata tagcggaaat aatagtatta acattaaccc ctttatagca
     3661 aaagataatg tgggtgttaa gaagctgata cttgaatatt ctgttgatat gattaactgg
     3721 ataaatattt gtgaaagagt cattgataaa cctgccagac aggtacgttt taatgaaaac
     3781 tgggatgttt cgtctttagc ggaaggagac tattttgtaa gagctcttgc cgaagatgaa
     3841 gcagggaata tgagcttacc ttctgaagct gccatatata ctgttgatca tacaaagcct
     3901 gctgcaccat ttgaccttac ttttactgca actgccggat acattgaagt aatgtggaag
     3961 tatttggctg aaaaagattt tagcttcttt aagctgtgga gatctgaaag tgaggacgga
     4021 acctatgaaa atattactcc gggttattat gtctatatgg gctacagaga tcgtacagtg
     4081 gagaccggaa aaacatacta ttataaggtt actgtagttg atgtggcaga taatgaaagt
     4141 gaacctagtg aaactattat aggaagcctc actgaagaca aaattgctcc aacaatttat
     4201 tttagaaacc ctttagaagg agatatcctt aactcaaaag ctctgttttc tattagtttt
     4261 agtgatgacc ttgcattgaa aagcatgaaa gttgaatacc ggaaagaagg ttccggagat
     4321 ggggatgaca gctgggaagt aataattacc agagactaca atcctgatgg tttgagtaaa
     4381 aaaagctatg caaatgaaga attttactgg gaaaataaag ggcttcccga aggccggtac
     4441 aaagtaagag ttactgttga agatagtgcc ggaaatatta cgggaccatt agttatgaca
     4501 tatcttgtca ataatgtagc tccgccaaaa ccaaccataa ctgcagaagc aggaggatgg
     4561 caggctagcc ttaactggat ttcggataat aatatagaag acttccatat gtataaactg
     4621 tatagaacca ctgtccttgg aggcagtgca agaaatattt tatcaaccgg tgaaaaatcc
     4681 ttcaatgata ttgggattca gccgggtatt agatattact actacgtaag ggcatatgat
     4741 atatatggaa attacagcca gagtgatacg gtttctgtaa ttccaaccgc tgaagataaa
     4801 atcctgcctg ttgcagatgc aggagatgaa catattttgg ttgtcggtat ggaaggattt
     4861 ttcgacggaa ccttatcaag tgacaaccat agaatagcat cctatcactg ggatttcgga
     4921 gatggaggta ccgctgtaac atcccggccc acacattcat acaatgcggt tggagattat
     4981 acggcaaccc tgacagtaac ggatcctgca gggaatacag ccgtagatac aaccggtgtt
     5041 aaagtagtat caattgatga ggtgggaatc cttgaagtaa aagtaattga tgatgtgaca
     5101 ggggaacttc tgcccggatc aagtgttgtt atagactatc ctgacggagc aactcataaa
     5161 accacaacaa actctgtagg cgctgcattt gttatagctc ctccgggaga ttatgaagtt
     5221 tatgcattca aaacagactt caggcctgcc tccgctaaag tcacaatggt aagaggtcag
     5281 aaaaacactg ttatgataag gctcccaaaa gggtcggtag tcacaggaga actgaaagca
     5341 gaaagaatgg atatagatga aattattgct gccggaattg acataacaga tcctgccaat
     5401 caaaacgcat ataaatttga aataggatta ggctttaata tgcctgatat accgattatt
     5461 gccacaaaac ataaattcat tcgcccggca aatgctcccc aaaattacac agtttccgag
     5521 gatggaagta aaatcattat tagggaacgt atagatgagg agggaaaagg aagcggaaga
     5581 attttcttcg gtgaaaagat tgacgcatct tttgatgttc ctgcaatggc ttatatggtt
     5641 atcccgggca aaaccacctg gcttaaggag ttctttgata taggattaac cattcaaaat
     5701 gcggcagggc ccgagtttgt tatcgaagat gcatgggcaa ggcttacaat tccggatgga
     5761 ctttcgcttg ctcctacaag agagggtcag catgaatacg ttgaaatggg gagtattccg
     5821 ggtggggaaa gcaaaagtgt aaattggata ataagaggag ataagaaagg agaatacagg
     5881 ctaaaagctg aatttaacgg tttattaatg ccttttgaag atgagataaa acaaatattc
     5941 agaaatgatg aacctttcag ggtatgggcg gaagatgcgg tgttaatgca cattataacc
     6001 caggacagag cagataaagg ttatccatat aatttgaaat tgggtatcga aaatatatct
     6061 gaaatatcat tatataacct ttctcttgag cttcttgagg aaacaaaggc aaattacatt
     6121 tatgccccaa atcaggaact gataaaatca gttgctgagc ttaagccggg agagacattg
     6181 tggggagagt atcaactgat ttcagcaata gaagggttat taagccttaa gcagtcttat
     6241 aacttaaaaa ccggtggaaa tacagacatt gaagaagaaa tgagtgccat atctgtcccg
     6301 gaaaactcac cggacaatgc tcccgtgtta aaccaggaaa ataaagatga cggtacagtc
     6361 aacttaagct gggaacaggt tgaaggtgcg cttggataca agatatatag aataagagaa
     6421 gacctgcgta tttccatgga tccggaagaa ttggtttata gctgtagact tgaaggtctg
     6481 cccggagaat tgtctgaaga catatctgta aatcttcctg aacctgacgg gccaaaggat
     6541 tatgtaatta atacacttat agcggatggg gaagatgtaa aagaagtaac tcttcatgca
     6601 ataaccggtt tttcctggat tgaaaatgca gctgagccca ccataaccgt tgaacctgaa
     6661 acccttattg tgggaagaga aacagaacta cttgtcactg caaaagaagg cggaaagcct
     6721 gttcatatca ttgctaatcc ggcagaaagg ccttcttacc gcatcaatgg aactattgat
     6781 ataggagact tagccaaaaa tcagagacta aacgataatg ggcaggtaag ggttgttgtg
     6841 aagcctgaaa agcccggacc cattgaaata aaatcctatc tcctgggtcg attcattacc
     6901 cggaaaacaa ttaatgcggt tttaccggaa gagcctaaac aacctcaggg gcttaaggct
     6961 tcgaagggtg atagaaaggc caagttgacc tggttcccca acagtgaacc ggaagtgaag
     7021 ggatactata tataccgggt aatagataac atatggacaa aaatacaccc gggaatttat
     7081 aaatgtgata gaagtgaagg acttgtcagc tatgaagtta caggattaaa tgacaacaca
     7141 acctacactt tccaggtatc tgcagtagat gcatttaaca gggaatctgt tccctcaaaa
     7201 cctgcacagg taacgctgac tatacctgaa gacacaatag caccatgggt tgttggcagt
     7261 aatcctgtca gccggcagaa ggatgtacct gttgacacta aaataataca aatctttttc
     7321 agcgagaata ttgcattatg ggaaacttat gagaatatcg aggttaaagc aggggacgtt
     7381 actgtagaat tcatagcaga agctgaaata ggaacaaata tccttactat tacattaaat
     7441 gaagaactgc cattaaaaac acattgccag gtttttattc ctgaaggtgc agtaagagat
     7501 aaatccttta atgaacttaa aagtgagaac aaaggtgact atgcctataa gttggaattt
     7561 tttactgaag ctgagaatga tacaatttgt cctatgttaa ttgctgcaag tccaaaggaa
     7621 aatgcccaat cagtaactgt tcattcaaca attttccttt attttagcga gaagattgat
     7681 gaaggaccgg cttatgataa tatcatgata actgcaaacg gcatagctgt tccacattca
     7741 aaagaaatta atggtgccaa gataatcatt aaaccattaa gcgagccaac ctataaaggt
     7801 gtgctgccat acaacagcaa gatagaaatc tttgtaccgg agggagcagt agttgatagt
     7861 gcaggcaatg tgttaaagaa tcaacatggt ttaaagtttt ataccgatac cgttccggat
     7921 ctaaatgccc ctaaaattat aagaatgaag ccctcggcca atagcagaga tttacctttg
     7981 gataccgagc ttaatatata tttcagcgag agaattaaag aagg
//
LOCUS       DAOSDD010000208         7965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3359_length_7965_cov_4.649178, whole genome shotgun sequence.
ACCESSION   DAOSDD010000208 DAOSDD010000000
VERSION     DAOSDD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_7965_cov_4.649178"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PK733_15220"
     CDS             <1..499
                     /locus_tag="PK733_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ11924.1"
                     /translation="QPFELIGQLKSKGVKLAIDVEADSFIDRNDAEAYIALTDVLFLN
                     QRAFDKYCGAQHYAETLEDLLSVGPRIVVVTRGADGCLVKTSKQEFTSPAYDVKVIDT
                     TGAGDTFNSTFLYGILSGWDECKSAQFANGAAALAIAEIGPRGGVRPIVEIEDFIRKN
                     TLSKE"
     gene            514..2025
                     /locus_tag="PK733_15225"
     CDS             514..2025
                     /locus_tag="PK733_15225"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ11925.1"
                     /translation="MQLEAILSVKDIEKSFPGNQVLKKVSMSVLPGEVMGLVGENGAG
                     KSTLMKIITGVYSFDAGEIMFEGRSVSFDNPKEALKQGIAIIHQEFNLFQNLSVAENI
                     FLDRSEYLGKLGKVQWKKMEKEAQAVLGELGANFDVDTLVSDIGVREQQLVEIAKAVS
                     SNSKVIIMDEPSAALPQNEVGHLFDVINILKSRGCAIIYVSHRMKEIEEICDRVTVLR
                     NGVNVGTLVMAKSNIQDIVTMMCGREIGEYYPHTLHVAGNTALKVSDLTTDFLKYISF
                     EIKEQEIIGLYGLAGSGSTELAESLFGLSKIDNGEIETSAGKAVKGKYNPKKAMELGF
                     SYVPPDRHREGIVKELSIEKNLILAQMSAYTKLGFMSRKKIEESSKSYIKQLNIRTTS
                     SKQYLLNLSGGNQQKVVLARWLDVGPRILILNEPTRGVDIGAKVEIYTLIDKLAKSGL
                     AILLISSEIPEVLGMCDRILVMNKGAISGEYENKGLEQETLLSAASGTKEGRL"
     gene            2025..2990
                     /locus_tag="PK733_15230"
     CDS             2025..2990
                     /locus_tag="PK733_15230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ11926.1"
                     /translation="MKNKVKKLLASHEFIVAAFVFIVYIAFSVISGNFAKVDNIKLIL
                     SQVAINGICAIGVSMVVFLGGIDLSSGSILALCAASSGMFINAGMPLYLTILISMGIG
                     ALCGLLNGFLVARFRIPPIIATLASMNVIRGVAIIITGGDWITGFPDSFKVIGQGRVL
                     GLPIPFIIFIVITILSSLMLRYLNIGRKIYAVGGNASAAELAGFNIVGIKLFAYILCG
                     IMIGLAGTVYASMVGTISASTTGGSLGFQVLAAALIGGLSINGGKGTTVGTCFGVILL
                     GIMRNGLILSKVSEYWIDAVTGTVILIALVINALQVIHRQRRISI"
     gene            2987..3937
                     /locus_tag="PK733_15235"
     CDS             2987..3937
                     /locus_tag="PK733_15235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ11927.1"
                     /translation="MNTKLRKLNNVPESRSIILLLGLLIIMITYFAFISKFFLTKNNI
                     INIMRQTAEMGIITIPTAILIISGGIDMSLGSTVGFCAISLGLMLKAGIPISLAVFLT
                     ILIGAMIGIFNGFFVAKLKIPGIVSTIGTMVLIRGLCYIVNKGNPVNGFSKGFIALGN
                     KTVINIPISFFVLILLYVAAAIIMKKSSFGVYTYALGNNEKTTRYCGINTDNFKWILY
                     IINGVVISIASVFLLARLGSAEATLGQNYDLDVLASVLIGGISILGGKGNIIGAFLGL
                     IVIGVLRNGLNIIGLSVLYQSIILGVLLILTAAKWKNIKN"
     gene            4094..5149
                     /locus_tag="PK733_15240"
     CDS             4094..5149
                     /locus_tag="PK733_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ11928.1"
                     /translation="MKKAVKLIGAILCIAILFAACSSGKTTTGTENTDTTNSSTKGNK
                     DDSKKELYFINPKSIGYAYWEFAKKGAEQAGTDVGVEVVFNGHTDIDSAKQINMIEDM
                     LTRGATGIAVAPNDAQAVASLFSRARSQGVNVVTFDSDAPDTERQYYVGPDTDKAMAE
                     KLIDIIAEDIGGKGEIAFMVAGLGAENQIAKMKAAKAKLEAEYPDIKLVATVSSNDEM
                     QVSLTNAQNLIKTYPNLKGIVGFAGGEAPSAAEAVEQAVKAGELKGGQISITGIGWPN
                     MCRNYIKNGTINKTLAWQSEQLGYASVFILKSLAKGTDIKGEMKMPNGKTIKIDGTNV
                     FTGLLEISKDNVDDYNF"
     gene            5166..5639
                     /locus_tag="PK733_15245"
     CDS             5166..5639
                     /locus_tag="PK733_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11929.1"
                     /translation="MKMKVVPIQEIDIRKYGRFYYLDCAAKRGDGWESTISSEEIMNS
                     PCHIGLVRSQSIPISISSLERHITTKEVMITTTTEVVVALATTDVDDSTVAPHINQVE
                     CISLPAGSILIINERIWHSACYGLTENALYYFIYSKTDEPVIWTKIKGGVCEITV"
     gene            5995..6276
                     /locus_tag="PK733_15250"
                     /pseudo
     CDS             5995..6276
                     /locus_tag="PK733_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            6610..>7965
                     /locus_tag="PK733_15255"
     CDS             6610..>7965
                     /locus_tag="PK733_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ11930.1"
                     /translation="MEQEKNRKAEPHYYSDRLKHNLSKIRFVPATVVEAPSGYGKTTA
                     VRDYLEAMLPQGTPVYWFTATNEVPAASFKRFCREIEKIDSHAGKRLLRIEFPSAATI
                     GEAIDALRSIRCMHETYLVLDNFQFLDEVLPPAFFAALLEHGGAGLHIIVVTQMLKRN
                     MLAVIAGHGVLHITAADLRLNVEDICRYYALVDVNITQEEAENVAYYTEGWIIAVYLQ
                     LCAFRETGTFSNAPGILVHMEHLIWNTLTEAQQTFLLYLSPFEMITVQQASCLIGRDV
                     LPEYAWDVLGSPFIQYDPVKRCYELHSILIDLLVQKRSDRGSAFERKCLLQAGDFCRD
                     DGQIAAALGFYVQVGDYERMLSLDLSHMTFETVNGASFDKLALDIAMNCPSDLKKAHI
                     MNMLRIAWTLLTFGINDAFEALMDELHTMLHAGDKVLPYCSSRNADSQLLGEWLLLSS
                     YR"
BASE COUNT         2493 a         1266 c         1759 g         2447 t
ORIGIN      
        1 acagcctttt gaactaattg gtcaattaaa aagtaaaggt gttaaactgg ctattgatgt
       61 agaagctgat tcatttatag atcgaaatga tgcagaagca tatattgcat tgacagacgt
      121 tttgtttttg aaccaaagag ccttcgacaa atattgtgga gcacagcatt atgcagagac
      181 acttgaagat ttactttccg ttgggccacg catcgttgta gtaacaaggg gtgctgacgg
      241 ttgtttagtg aaaacctcaa agcaggaatt cacttctccg gcctatgatg taaaagtgat
      301 agatacaaca ggtgcagggg acacctttaa tagtacattt ctctatggaa tactatccgg
      361 gtgggatgaa tgcaagtcag cacaatttgc taatggagca gcagctttag ctattgctga
      421 aatcggacct cgtggaggtg tgcgaccaat agtagaaata gaagatttta tcagaaaaaa
      481 cactttgtcg aaggagtaga aaaggaggag accatgcaac ttgaagcaat tttatcagtt
      541 aaagatattg aaaagtcatt tccgggtaat caggtgctga aaaaagttag catgtctgtt
      601 ctacctggtg aagtgatggg gctggtcgga gaaaatgggg caggtaagtc aacattgatg
      661 aaaattatta ctggtgttta ttcttttgat gctggtgaga ttatgtttga agggcgcagt
      721 gtttcatttg ataatccaaa ggaagcccta aagcagggga ttgcaattat tcatcaggaa
      781 ttcaatctat ttcaaaattt atcagtagca gaaaacattt ttcttgaccg tagcgaatat
      841 cttgggaaat taggaaaagt acaatggaaa aaaatggaga aagaagccca ggcagttcta
      901 ggggaattag gagcgaattt tgatgtagat actcttgtgt cagatatcgg tgtaagagaa
      961 cagcagttgg ttgagattgc aaaggctgtt tcctctaact caaaggttat tataatggac
     1021 gagccttcag ctgcactacc tcaaaatgag gttggtcatt tatttgatgt tatcaatata
     1081 ttaaagagca gaggttgcgc gataatttat gtttctcacc gaatgaaaga aattgaagag
     1141 atttgtgatc gtgtaacagt attaagaaat ggtgttaatg tcggaacatt ggtcatggca
     1201 aaaagcaata ttcaagatat tgtaaccatg atgtgtggac gtgaaatagg ggagtattat
     1261 cctcataccc ttcatgtagc aggaaacaca gcacttaaag tatctgatct aacaacagat
     1321 tttttaaaat atatttcttt tgaaattaaa gaacaggaaa taataggact ttatggactt
     1381 gccggttctg gttctactga actggctgaa agtctctttg ggctttcaaa gatagataat
     1441 ggggagatag aaactagtgc cggaaaggcc gtgaaaggca aatataatcc taaaaaagca
     1501 atggaacttg gtttctctta tgttccccct gatcgtcatc gtgaaggaat cgttaaagaa
     1561 ctttccatcg agaaaaacct aattcttgct cagatgtctg cctacactaa gttaggattt
     1621 atgagccgga aaaaaattga agaatcttct aagagttata ttaaacagct aaatattagg
     1681 acaacttcca gtaaacaata cctgttaaac ctttccggag gaaaccagca aaaagttgtt
     1741 ctagctcgct ggcttgatgt gggaccacga atattgattc ttaatgagcc tacccgaggt
     1801 gtagatattg gtgccaaggt agaaatttac acgttaattg ataaattagc aaaatccggg
     1861 ttggccatat tgctcatttc ttcagaaatt ccagaggtac tgggtatgtg tgatcgtatc
     1921 ttggtaatga acaaaggggc tattagtgga gagtatgaga ataaagggtt ggaacaggaa
     1981 acacttctta gcgcagcgtc aggtacaaag gaggggaggt tgtgatgaaa aataaagtaa
     2041 aaaaactttt agcatctcat gagtttattg tggctgcctt tgtttttatt gtttacattg
     2101 ctttttcagt tatatcggga aactttgcta aagttgataa tataaagttg atattgtctc
     2161 aggttgctat taacggtata tgtgcaatag gtgtttctat ggttgtgttt ctaggcggca
     2221 tagatctttc atcgggttca attcttgccc tatgcgctgc aagctcaggt atgtttataa
     2281 atgctggaat gccgctatat ttaacaatac tgataagcat gggaatagga gcattatgtg
     2341 gattgttaaa tggtttcttg gtagcacgat ttcgtattcc tccaatcatt gcaactcttg
     2401 cttctatgaa tgttatccga ggtgttgcca ttatcattac aggtggagac tggattaccg
     2461 gttttcctga ttcttttaaa gtaattggac agggaagagt gcttggacta cctataccgt
     2521 tcatcatttt tattgtcatt acaattctgt catcgttgat gttaagatat ctaaatattg
     2581 gacgtaaaat ttatgcagtc ggtggaaatg ctagtgctgc tgaactggca ggtttcaaca
     2641 tagtgggtat aaaattgttt gcctatatat tgtgtggtat tatgattggt cttgccggaa
     2701 ctgtttatgc gtccatggtt ggaactatta gtgcttcaac aactggtgga tctctagggt
     2761 ttcaggtact tgcagctgct cttataggtg gactgagtat taacggcggt aagggtacta
     2821 cagtaggcac atgctttggt gtaattttac tgggaattat gagaaatgga ttgattcttt
     2881 cgaaagtatc tgaatattgg attgatgctg ttaccggaac agttattttg attgcattgg
     2941 taataaatgc actgcaagtt attcataggc aaaggaggat atcgatatga atactaaact
     3001 tagaaagtta aataacgtac ctgagtcacg cagtattata ctgcttttag gattgctaat
     3061 tattatgatt acctactttg cttttatatc taagtttttc ctaaccaaaa ataatattat
     3121 taacataatg cgtcagactg cagaaatggg tattattact atacctacag ccattcttat
     3181 tatttcaggt ggcatcgata tgtctcttgg ctcgacagtg ggattttgtg ccatatcact
     3241 tggtttaatg cttaaagctg gtataccaat ttcattagca gtatttttaa ctattcttat
     3301 tggcgctatg attggtattt ttaacggctt ttttgtagca aagctcaaaa ttccgggcat
     3361 tgtatctact atcggcacta tggttttaat acgtggattg tgctacatcg ttaataaggg
     3421 caatcctgta aatggcttct ctaaagggtt tattgcactg ggaaataaaa cagtaattaa
     3481 tattccaatt tcattttttg ttcttatttt attatatgta gcagcagcaa taataatgaa
     3541 aaaatccagc tttggtgtgt atacctatgc gttaggtaat aatgaaaaaa caacacgtta
     3601 ttgtggtatc aatacagata actttaaatg gatattatat attattaatg gtgtggtgat
     3661 tagtatcgca agtgtgtttt tacttgcaag acttggcagt gcagaggcaa cacttggcca
     3721 aaactatgac ttggatgtgc tcgcttcagt gcttataggg ggcataagta tattaggtgg
     3781 taaaggtaat ataattggtg catttttagg attaattgta atcggtgttt taagaaatgg
     3841 actaaatata atcggcttat cggtgctcta tcaatcgatt attctaggag tgctgttaat
     3901 tcttactgca gctaaatgga aaaacattaa aaattaaatt gttggttaaa tttacattaa
     3961 tattatgatt tttataaagt gaattctcta actaatattc ttgggaatct attaatttgg
     4021 aatcaattaa tttggaatct attagttaaa aattaataat agattaaaaa aattaactaa
     4081 gggggaaatg aaaatgaaaa aagcagtaaa acttatcggc gcaatactct gtatagctat
     4141 tcttttcgca gcatgcagca gtggtaagac aacaaccggt acagaaaata cggacacaac
     4201 aaattccagc acaaagggta ataaagatga tagtaaaaag gaactgtact ttatcaaccc
     4261 gaaatcaatt ggatatgctt actgggaatt tgcaaaaaaa ggtgcagaac aagcaggtac
     4321 tgacgtagga gtagaagttg tattcaatgg tcatactgat attgattctg ctaaacaaat
     4381 taacatgatt gaagatatgc ttactcgcgg ggcaacaggt attgcagtag ctccaaacga
     4441 tgcacaggcg gttgcgtctc tattctcccg tgctcgttcc caaggtgtta atgttgttac
     4501 ctttgattcc gacgcacccg atactgaaag acagtattac gttggaccgg atacagataa
     4561 ggcaatggct gagaagctaa tagacattat tgcagaagat attggcggta aaggcgaaat
     4621 cgcatttatg gtagctggtc ttggtgctga aaatcagatt gcaaaaatga aagcagcaaa
     4681 agcaaagctg gaagcagagt atcccgacat taaattagtt gcaactgttt ccagtaatga
     4741 tgaaatgcag gtttcattaa ccaatgccca gaatttgatt aagacttatc ctaatcttaa
     4801 gggcatcgtg ggatttgcag gcggagaagc tccatcggct gcagaggctg ttgaacaagc
     4861 tgttaaagct ggagagctta aggggggtca aattagtatc actggtattg gctggcctaa
     4921 tatgtgccgt aattatatca aaaatggtac cataaacaaa accctggcat ggcagtctga
     4981 acagctgggc tacgcaagtg tatttatttt aaaatccttg gcaaaaggca cagatatcaa
     5041 aggtgaaatg aagatgccta atggtaaaac aattaagatt gatggtacca atgtttttac
     5101 gggacttcta gaaatatcta aagataatgt tgatgattac aatttctaaa aggtagagtg
     5161 atgtaatgaa gatgaaagta gttcctatac aagaaataga cattaggaaa tatgggcggt
     5221 tctattattt ggattgtgcc gcaaagcgtg gtgatggttg ggaatctacc atctcatctg
     5281 aagagattat gaactcacct tgccatattg gtttggtgcg ttcgcaaagt attcctattt
     5341 ccatttctag tcttgaacgt cacattacca ctaaagaagt aatgattaca actactacag
     5401 aagtggtagt agcattggca acaacagatg tggatgattc gactgttgcg cctcatataa
     5461 atcaagtgga atgtatatca ttacctgcag gaagtatttt aattatcaat gaaagaatat
     5521 ggcattccgc ttgctatgga ttaactgaaa atgctcttta ttattttatc tattccaaaa
     5581 cagatgaacc tgtgatatgg actaaaataa agggtggagt ttgcgaaatt acagtctagc
     5641 aatgcattaa aatatattaa ctagggctta tgaaactgca gacgcaggtg atgagtcagg
     5701 ccagaggatg tacttcagtg tattgtatgg agatatcttg gcaaagctca gggtaaaagg
     5761 tgtttcgtcg gaagtaatat ttgacatctt atagtagatt aaacaataga aatataaaaa
     5821 gaatttgacc gctgaattgg gagaaataac tccagattgg cggtcatttt tgtaagttgt
     5881 acgaagcaat tgcggacttt tactaaatca tagtaaatta taattggcat tttgatctat
     5941 gcggtataaa cttataatac aaggtcgata tttcatagac caaggaggtt tataatgaaa
     6001 gattacaaac ttacagaaag tgaagaaaag tttgcagagt taatttggca gaacgagccg
     6061 attggttttg gtgatcttgt gaagatatgt gaaaaagaaa tgaattgtaa aaagtcaaca
     6121 acatatacag tactgaaaaa gttatgcgaa aagggcattt tccaaaatga aaatactgtg
     6181 gtttcatctc taattacaaa ggatgaatat tacgctaagc aaagtatacg gtttgttgag
     6241 gatacctttg gtgggtcttt gcctaaattt ttaacagtca tctcctaaca gaacctcctt
     6301 aaaaatgggg aaactcaaat caataaaacc ttgtattatt cgctttgctg gaatataatt
     6361 gtaatagaaa tatcctgtat tttgaccggc gttgtacata ctaagaataa cattattaag
     6421 tatctacagc aaaaacgcag caaaagaata aaacacagca agagaataaa acactataat
     6481 tttgaatttc atttaaatag aacacgcctt gcaaaatcat ttttgtgtta tatctaacaa
     6541 gaaaagaaaa ttaatattca gaaaggatag tatgatttag ttgttacaac tacatcgtac
     6601 aaggttatta tggagcagga aaaaaaccgt aaagccgagc cgcattatta ttccgacagg
     6661 cttaaacaca acttgtcaaa gatacgcttt gtacctgcca ctgttgtcga ggctccatct
     6721 ggctatggca agactacagc ggtacgtgat tatttagagg caatgcttcc ccaaggtacg
     6781 cctgtttatt ggtttactgc aacaaatgaa gtgcctgctg caagttttaa gcgtttttgc
     6841 cgggagattg agaagattga cagccatgct ggtaaacgcc ttctcagaat tgaatttcca
     6901 agcgctgcca caattggtga agcaatagat gctctaaggt ccatccgatg tatgcatgaa
     6961 acctatcttg ttcttgataa tttccagttt ttagacgaag tattgccacc tgcttttttt
     7021 gctgcccttt tagagcatgg cggtgcaggc ttacatatta tcgtcgtgac ccaaatgctc
     7081 aagcgaaata tgcttgctgt cattgcaggt catggggttt tacacattac tgcagccgat
     7141 ttgcgtctga atgtcgagga catctgccgc tattatgccc ttgtggatgt gaatattacc
     7201 caggaagaag cggagaatgt ggcatattac actgaaggct ggatcatcgc cgtctattta
     7261 cagctttgcg ctttccggga aacaggcaca ttctctaatg cacctggtat tctggttcat
     7321 atggagcatt tgatatggaa tacattaacc gaagcacagc aaacctttct tttatacctg
     7381 tcaccgtttg agatgattac tgtacaacag gcaagttgcc taatagggcg cgatgtgctg
     7441 cccgagtacg cttgggatgt actggggagt ccgttcatcc aatatgatcc agtaaagcgg
     7501 tgctatgaat tacacagcat tctgatcgat ttgctggttc agaagcgtag tgaccgtggg
     7561 tccgcatttg aacgtaaatg tttgttgcag gcgggagatt tttgtagaga cgatggccaa
     7621 attgcagcag cgttgggttt ttatgtgcaa gttggagatt atgagcgcat gctttcgctt
     7681 gatctctcac atatgacttt tgaaacagtc aacggtgcat cttttgataa gctggctctt
     7741 gatatagcaa tgaattgccc ctcagacttg aaaaaggctc atattatgaa catgctccga
     7801 attgcatgga cgcttcttac ttttgggata aacgatgcgt tcgaagcact gatggatgag
     7861 ttacacacaa tgcttcatgc cggagacaag gtgttgccgt attgctccag tcggaacgcc
     7921 gattcgcagc tgctaggcga gtggctgctt ctttcttcat ataga
//
LOCUS       DAOSDD010000209         7956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3363_length_7956_cov_5.216428, whole genome shotgun sequence.
ACCESSION   DAOSDD010000209 DAOSDD010000000
VERSION     DAOSDD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3363_length_7956_cov_5.216428"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..899
                     /locus_tag="PK733_15260"
     CDS             258..899
                     /locus_tag="PK733_15260"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ11931.1"
                     /translation="MSGDTRLIKEKVFDSIFEDIIRGEYPVNTIINERMLIEKYKVSK
                     SPVREALIELCKENILKSIPRMGYQVVQISPKEIYDILELRLIIEVAALKKTFALLDD
                     NIIEKLEKNIQRTLEFIGEKDIVNCWNRNIGFHLLLCSFCNNNSIYNTLNDILKFCSR
                     GAIQYYQKTWDSSQSVDRNNHISLIDALRKRDFKLAEQILGKDVSSMKDVFFN"
     gene            936..2216
                     /locus_tag="PK733_15265"
     CDS             936..2216
                     /locus_tag="PK733_15265"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOJ11932.1"
                     /translation="MSLLEKTYSEYVQILKEELVLALGCTEPTCIAYVCAKARQVLGE
                     FPEEVVVESSGNIIKNVKGVIVPNTGNLRGIDAAAIIGIVGGDPDKELEVLTGIEQKD
                     IDKAAELMKKNFCKVRLLKTKANLHVIARVKSENHSAMVEIVHTHTNIVRIEKDGELL
                     FKKDYDCSKCNDGLTDRSGLNVKDILEFADTFELDDLKGIILQQINYNSRISQEGLNH
                     LYGAGVGANLIKHYGCDIKVVARAAAAAGSDARMSGCILPVVINSGSGNQGMTVSLPV
                     IEFAKHLGASEEKLIRALVLSNLVAIHQKTAIGRLSAYCGAVSAACGSGAGITYLHGG
                     TYDQICKTITNTLANVAGIVCDGAKPSCAAKIASAVDAAIMAHYLSMENECFRPGEGL
                     VKGDVEETISSIGRLGRDGMRETDREILKIMVEG"
     gene            complement(2624..3532)
                     /locus_tag="PK733_15270"
     CDS             complement(2624..3532)
                     /locus_tag="PK733_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HOJ11933.1"
                     /translation="MGLFSKKPPCPICGGKISWLLPSKIEDEYICSTCYNKIDMESEK
                     ESQLTMQGFREYLAFYDQNQLLKNQFVISERIDFGLWDTKIIFDHQNKLFCMSKNPDK
                     TVFEGKQLKSFTIKEDNTPLFEGSAAGILRYTSTVPERTMAMAPQISQFIMNKQMVRA
                     LDKLDDGKENKSAPVQYFNVPEPFHAFNVELHFDHPYWTVIKCDMNGPQFDNTHPNVK
                     DYLHSYQRSIEELEKLVASLRTVAFPGAPEQSVGLGTAGTQAVHTTMAPPTDAIEEIR
                     KYKVLMEDGIISQQEFDAKKRQLLGI"
     gene            complement(3560..5254)
                     /locus_tag="PK733_15275"
     CDS             complement(3560..5254)
                     /locus_tag="PK733_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOJ11934.1"
                     /translation="MREKVCRLILCFALLFALPLPAYAANQVDTMDIQAVLYEDGSMH
                     ITQNWSGRFEEGTESYIPMNVPDYLTISELTVSDQNGSYETVPDWNLDWSFEEKTGKC
                     SIHDTDSGYEICFGISRYGQNRYTIAYKLDNVIGGYSDRDGVNFRFVNDSMNTTPTDV
                     TVQIRLSDSTPITNEIADVWGFGFAGKVTFENGSIVARTDTSLSTENHVTVLFSLEKG
                     ILSPSRQEAGSFEEMREKAFEGSDYDSIGDSGEEVSIFTALTVTLLSIGLPIALIIWI
                     RRMKKKSVEKKQQRFSERFGYFRDIPNGGNLSATYALGRMFDVCEDGVILATGMLRLI
                     QLGRLSPVETQQVGFMGKTKETVSLRLMGSHHDKMNEYDEYLYTVLESAAGSDAILQA
                     KELEHFANQKDTLLRAYIQKQDSAGRAYLNQKHCLKHWNIPAKLTDLTPTGEQELGEL
                     MGLKRYLTDFSLINQRGVKEMPIWQELLTYAMLFGIADQVAEQMKELYPALSSELTDY
                     SQSMVTAYSYHYLLYSNMKKAEEQREQEKRSGGGGGFASFSGGSGSIGGGSGGGTR"
     gene            complement(5263..5817)
                     /locus_tag="PK733_15280"
     CDS             complement(5263..5817)
                     /locus_tag="PK733_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ11935.1"
                     /translation="MQPTFIVIIAIAAVIVLWAISTQRRLVVLDENISNAMSQIGVQL
                     SSRFDALTALLDLTKGYAKHESETLIETIKSRRSVITAKSTPDDVLRQEGIISEALGR
                     IAMVTEQYPELKANQTYIKTMDAVQTFENMVRTSRLIYNDSVTKLNREIRMFPISMIA
                     GMLGFRQREYLVEQAGKADMPSMK"
     gene            6077..6802
                     /locus_tag="PK733_15285"
     CDS             6077..6802
                     /locus_tag="PK733_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ11936.1"
                     /translation="MLQIAVCDDNIDELSNMVQLINLYRASRNFSCEYAAFPNGFDLV
                     SALEKGKRFDIYCLDIIMPGFMGIDVAKEIRGFDKVAPILFFTSSPEFALESYSVKAI
                     NYVLKPISKEKLFFTFDEMLEQIKAEKNENAVIVKSNEGIQKILISNLVFAEVIGRNV
                     LYHLRSSKVIECTEPFSPVCDNLLKYGCFIKPHRSYLVNMQYVDTIENHQVSLQTLSS
                     VPVAQGKAKEIKEQYLAYQMEGE"
     gene            6807..>7956
                     /locus_tag="PK733_15290"
     CDS             6807..>7956
                     /locus_tag="PK733_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOJ11937.1"
                     /translation="MIETVIEFSRYLIALLFGATVAVSFAGMARTRKNYLALGCFAAI
                     LFMLQIASLRLWGLEMTIKIYPLLSHLPVAVFIALYLKRPWLFSLTSMFVSFLCCQPL
                     RWIGAVVGEVFDSAPMDHVGYIVAAFLMYYFLHKYAVKSVRHLMERSVKSCLLFGAMP
                     ASYYLFDYAATVYTDFMYSGTRAAVQFMPFVTSAFYFVFVLLYYAETQKQASLQRERG
                     MLDTQLRLAQTEFASLRQMQQNAAAYRHDMRHHFALLQGLASKEHIEELKEYLRTAQS
                     DMDAITPTRFCENETVNLILSAFAGKAKQSGILLTVDAKLPDALPFSDTELCSLLSNA
                     LENAIQASENIADSNKRYIKLHMYSKNNKLCIDIRNSYHVEPIFHQGLP"
BASE COUNT         2112 a         1724 c         1865 g         2255 t
ORIGIN      
        1 agttttgtat gttgagttct cctaataatt atggtgttaa aagtctaaag acgggcatat
       61 acattttgtt gtgccaaaaa ataatacgta ttgactcttg atattatcag tgatattatc
      121 aataatataa tcataaaatc aacaatagaa tattttaaaa gcgtatcagg attttgagct
      181 ataaagctgc aatgcaataa tttcaaaaaa tctgcaggta cagtcaagaa gcaattttgc
      241 aggcggaggt acatagagtg tcaggtgata caagactgat taaagaaaag gtatttgata
      301 gcatttttga ggatattatc cggggagagt atccggttaa tactattatt aatgaaagaa
      361 tgctgattga gaaatacaag gtcagcaagt ctccggtccg tgaggctctg atcgagttgt
      421 gcaaggaaaa tatattaaaa agtatcccac gtatgggcta tcaggtagtt caaatctcac
      481 ctaaagaaat ctacgatatt ttggaattgc gacttattat agaagtggcg gcattgaaaa
      541 aaacattcgc cttgttggat gataatataa ttgaaaagct tgaaaaaaac attcagagaa
      601 cacttgaatt tattggcgaa aaagatattg tgaattgctg gaacaggaat ataggatttc
      661 atttgctgct atgcagtttt tgcaacaata acagtatata taatacctta aacgacatct
      721 tgaaattttg ctcaaggggc gctatacagt attaccaaaa gacgtgggac agcagtcaga
      781 gtgtggatcg caataatcat ataagcttga tcgatgctct ccgtaaaagg gattttaagc
      841 tggcggaaca aatattgggt aaggatgtct catctatgaa ggatgtattt tttaattaaa
      901 atgtgataat aaagaaagag gaagtgatta cccggatgag cttattggag aaaacataca
      961 gtgaatacgt ccagatttta aaagaagagc ttgttctggc attgggctgt accgagccta
     1021 cctgtatagc ttatgtatgt gcaaaggcac ggcaggtatt gggggagttt ccggaagaag
     1081 tagtggtgga gagcagcgga aacataataa aaaatgttaa gggtgtaatt gttcccaaca
     1141 caggtaacct tcgcggaata gatgctgcag ctataatcgg cattgtgggg ggagaccctg
     1201 acaaggaact tgaagtttta accggaatcg agcaaaagga tatagataaa gcagcagaac
     1261 tgatgaaaaa gaatttttgc aaggtaaggc tgcttaaaac taaagcgaat cttcacgtta
     1321 tcgccagggt aaaatctgaa aaccacagtg cgatggtgga gatagttcat acacatacaa
     1381 atattgtgcg tattgaaaaa gacggcgagc ttttattcaa gaaagactat gattgctcta
     1441 aatgcaacga tggcctaaca gacagatcgg ggctaaatgt gaaggatata ttggaatttg
     1501 cagatacctt tgaactggat gacttgaagg gaattatttt gcagcagata aactataaca
     1561 gcagaatatc gcaggaaggg ttgaaccacc tgtatggggc tggtgtagga gccaatctga
     1621 tcaagcatta tggctgcgat atcaaagttg ttgccagagc agcagctgct gcagggtcag
     1681 atgcaagaat gagcgggtgc atccttcctg ttgtcataaa ttccggaagt ggaaaccagg
     1741 gcatgacagt ttcactgcct gtaatcgaat ttgcaaagca tctgggggca agtgaagaaa
     1801 agctgatccg ggcgcttgtt ttaagcaatc ttgttgctat tcatcaaaaa actgcaatag
     1861 gcaggctttc agcctattgc ggagcagtca gcgcagcatg cggcagcggt gcgggaatta
     1921 cttacctgca tggtggtacg tatgatcaga tttgtaaaac cataaccaat accttggcga
     1981 atgttgcagg tattgtgtgt gatggagcaa aaccctcgtg cgcagccaag atcgcatcag
     2041 ctgttgatgc ggcgataatg gcacattacc tctcgatgga aaatgaatgc ttccggcctg
     2101 gtgaggggct ggtaaaggga gatgtggaag aaaccatcag cagcattggc aggctgggac
     2161 gagatggtat gagagaaaca gaccgggaaa ttttaaaaat catggtggaa ggttaggcct
     2221 ggaccggact ctgttgacat gactatttta ttatcttggg agatgatatt aagatgatag
     2281 aagatttaaa ctatctgatg attattacta tgcaattagt gttgcagcca gtacagtaca
     2341 taaatttctt aggtctacct atattaaacc tgagcaaata attgggttag ggaaggtgct
     2401 acactttctt gaacaactgc caatttcaat ggaagggtta tatagaccgc ctgcacattt
     2461 ccggcctttt catttgcgct gagggtgtag gagatggcga ccgatatgtt gcctgcacca
     2521 aagctctgta cctgtttgcc gctgccgtag tcttagcaag cgtaaatcat agcaggcgta
     2581 aatcatgcca tcccccttct tttcatatgg atatagcaat ttttcaaatt ccaagcagtt
     2641 gccgtttctt tgcgtcaaat tcctgttggg aaatgatgcc atcctccatg agcaccttgt
     2701 attttctaat ttcttcaatg gcatcggtag ggggagccat ggtcgtatgt actgcttgcg
     2761 ttcccgctgt gccaaggccg acagactgct ccggtgcgcc cggaaaggcc accgtcctga
     2821 gcgaagccac cagcttttca agctcctcta tgctgcgctg gtaggagtga aggtagtctt
     2881 tcacattcgg gtgggtatta tcaaattgtg ggccgttcat gtcgcattta atcaccgtcc
     2941 agtagggatg gtcgaaatgc agctccacgt tgaacgcgtg aaaaggctcc ggcacattaa
     3001 aatactgcac cggtgctgac ttgttttcct tgccgtcatc cagcttgtca agagcacgga
     3061 ccatctgctt gttcatgata aactggctga tttgcggtgc catagccata gtgcgctccg
     3121 gcacggtgct ggtatagcgg agtatacccg ccgccgaacc ctcgaacagt ggagtgttgt
     3181 cctctttaat ggtaaaggat ttcagctgct tgccctcaaa cacggttttg tcggggtttt
     3241 tgctcatgca gaacagtttg ttctggtgat caaagatgat tttagtatcc caaaggccga
     3301 agtcaatccg ctcggaaatc acaaactgat ttttcagcag ctggttttga tcgtaaaacg
     3361 ctaaatattc ccgaaagccc tgcattgtca gctggctttc cttttcgctc tccatatcaa
     3421 tcttgttgta gcaagtgcta cagatatatt cgtcctcaat tttcgatggc agaagccacg
     3481 aaatcttgcc gccgcagatg gggcaagggg gttttttaga aaataagccc ataatcaaat
     3541 cctcctatat tttaaatcat tacctcgttc cgccgcctga accgccgccg atagagccac
     3601 tgcccccgct aaaggaagca aatccgccgc ctccgccgct gcgcttttcc tgttcgcgct
     3661 gctcttcagc tttcttcata ttgctgtaaa gcaagtagtg ataggagtag gcggtaacca
     3721 tgctttggct gtaatcggtc agttcggaag aaagcgccgg gtatagctct ttcatctgct
     3781 ccgctacctg atcggcgatg ccaaacagca tggcataggt cagcagctct tgccaaatcg
     3841 gcatttcctt aacgccccgc tggttaatca gtgaaaaatc cgtcagataa cgtttgagtc
     3901 ccatcagctc gcccagctcc tgttcaccgg ttggcgtcag gtctgttagt tttgcaggta
     3961 tattccaatg tttcaggcag tgcttttgat tcaaatacgc tctgccggca ctgtcctgct
     4021 tttgaatata ggcgcgaagc agtgtatcct tttgatttgc aaagtgttcc agctcctttg
     4081 cctgcaagat ggcatcagaa ccggcagcgc tctccaatac cgtataaagg tactcatcat
     4141 actcattcat tttgtcatga tggctgccca tgagccgcag gctgactgtt tctttggtct
     4201 tgcccataaa gcccacctgc tgtgtttcca ccggtgacag gcggcccaac tgaatcagcc
     4261 gcagcatccc tgtggcgagg ataacgccat cttcacacac atcaaacatt ctccccagcg
     4321 cataggtggc gcttagatta ccaccgttgg gaatatccct gaaatacccg aaccgttcgg
     4381 agaaacgttg ctgttttttc tctacgcttt tctttttcat cctgcgaatc cagataatca
     4441 gcgcgatggg aagacctata gaaagcagtg tcactgtaag cgctgtgaat atagatactt
     4501 cctcgccgct gtccccgatg ctatcgtagt cactgccctc aaaggctttt tcacgcattt
     4561 cttcaaagct ccccgcctcc tgccgcgagg gggacaggat tcctttctcc aaagaaaata
     4621 gtaccgtcac atggttttct gtggagagcg aagtatctgt gcgggcaaca atacttccat
     4681 tctcaaacgt taccttgccc gcgaagccaa atccccagac atcggcaatt tcattggtga
     4741 tgggtgtgct gtccgaaagc cggatttgaa ccgttacgtc ggtgggggtt gtattcatac
     4801 tatcattgac gaatcggaaa tttacgccgt ccctgtcgct gtaaccacct atcacgttat
     4861 ccagcttata ggcaatggta tagcggtttt gcccgtaccg gctgatgccg aagcagattt
     4921 catacccgct gtccgtatca tgaatgctgc attttcctgt tttttcttca aaactccagt
     4981 caaggttcca atccggcacc gtttcatagc tcccgttttg gtcggagact gtgagctcac
     5041 tgatggtcag ataatcgggc acattcatgg gaatgtagct ttctgttcct tcctcaaagc
     5101 gccctgacca gttctgtgtg atgtgcatgg agccatcctc ataaaggaca gcctgaatat
     5161 ccatggtatc cacctgattc gcggcataag cagggagagg gagggcaaat aacagggcaa
     5221 agcatagaat cagtctgcac actttttcgc gcatagggca cctcatttca tacttggcat
     5281 atccgccttg ccagcctgct caacaagata ctctctttgc cgaaagccta acatcccggc
     5341 aatcatagag atcgggaaca ttcggatttc acggttaagc tttgtcacac tgtcgttgta
     5401 gatcaggcgg ctggtgcgca ccatgttttc aaaggtttgc accgcgtcca tggttttgat
     5461 atatgtttgg tttgccttta attcggggta ttgctccgtc accatggcga tcctgcccaa
     5521 ggcttcggag ataatccctt cttgccgcag cacatcatcc ggtgtggatt ttgctgtaat
     5581 tacacttctt cttgatttga ttgtttcaat cagcgtttcg ctttcgtgct tggcgtagcc
     5641 ctttgttaaa tccaaaagag ccgttagggc gtcaaagcgg ctggaaagct gtactccgat
     5701 ctggctcatg gcgttgctga tattctcatc tagcacgacc agcctgcgct gggtggagat
     5761 tgcccataga acaatcaccg cagcaattgc aataattaca atgaatgttg gttgcatagt
     5821 catttcctcc tcgttttttt gaaatacgga tgtggatatt ccacatccgc ctttgttatg
     5881 gtattgcttt ttacgttaat gttgtttccc ctttaaaaat tttgttttac atctttatgc
     5941 tatcaaaaat ttcactaaaa aactgtccat tgcacgaaac atgatttttt cgacatgaat
     6001 catagtttcg ggaattcgat ttttatggta aacttagctt ggagaaatag aataatagag
     6061 aaatggaggg gtacttatgc tgcagattgc ggtctgtgat gacaatattg acgagctatc
     6121 caatatggtt cagcttataa acctatatag ggcatcaaga aattttagct gcgaatacgc
     6181 cgccttccca aatggatttg atttggtttc ggctttggaa aaaggaaagc ggtttgatat
     6241 atattgcctg gatattatta tgccgggctt tatgggcatt gatgtcgcaa aggaaatccg
     6301 tggttttgac aaagtcgcac cgattttatt ctttacctca tcgcctgagt ttgctttgga
     6361 aagttattca gtgaaagcca tcaactatgt actaaagcca atctcaaaag aaaaattgtt
     6421 cttcaccttt gacgaaatgt tggagcagat aaaagcagaa aaaaatgaga atgcggttat
     6481 cgtaaagagt aatgagggaa tacaaaaaat attgatctcc aatttagttt ttgccgaggt
     6541 catcggcaga aatgtgttgt atcacctgcg ctccagcaag gtaatcgaat gtacagaacc
     6601 tttttcccct gtctgcgata atcttctgaa atatgggtgt tttatcaaac cccaccgttc
     6661 ctatcttgtg aatatgcagt atgtggatac catcgaaaat catcaggtat ccttacagac
     6721 tctttcctct gtccctgttg cacagggcaa ggcaaaggag attaaagagc agtatcttgc
     6781 ctatcaaatg gagggggaat aagcgaatga tagaaacggt cattgaattc tctcgttact
     6841 tgattgccct gctgttcggt gcgacggttg cggtcagttt cgccggaatg gcgcgtacac
     6901 ggaaaaatta tctggctctt gggtgctttg ccgccatttt atttatgcta caaatagcaa
     6961 gtctgcggct ctggggcctg gagatgacga taaaaatata tccgctgctt tcccacttgc
     7021 cggttgctgt ttttatcgcc ctttacttaa agcgcccatg gcttttttca ctaaccagta
     7081 tgtttgtctc cttcctgtgc tgccagcctc tccgctggat cggtgctgtt gtaggcgaag
     7141 ttttcgacag tgctcccatg gatcatgtcg gttatattgt ggctgcgttt ctgatgtact
     7201 attttctgca taaatatgcg gtgaaatcgg ttcggcatct gatggagcgc tcggttaaat
     7261 cctgccttct gtttggtgcc atgccggctt cttactatct attcgactat gccgccactg
     7321 tttacaccga ttttatgtac agtggaacac gcgcagctgt acaattcatg cccttcgtga
     7381 catcggcatt ctattttgtg tttgtcctcc tgtactatgc cgaaacgcaa aaacaggcaa
     7441 gcctccaaag agagcggggt atgctggata cgcagctacg gctggcgcag acagagtttg
     7501 cttctttgcg gcagatgcag cagaatgctg ctgcctatcg gcacgatatg cgtcaccatt
     7561 tcgctctttt acaggggctg gcctcaaagg agcatataga ggagctcaag gagtatctgc
     7621 ggactgctca gtccgacatg gacgctatca cgcccacacg cttttgcgaa aatgaaactg
     7681 tcaatttgat tttgtccgct ttcgccggca aagcaaaaca atcgggaatt ctgttgacgg
     7741 tagatgcgaa gctgcctgac gcgcttccct tcagcgacac cgagctttgc tcattattgt
     7801 caaacgcttt ggaaaacgcc atacaagcct cggaaaatat agctgacagc aataaacgct
     7861 atatcaagct gcatatgtat tccaaaaata ataagctgtg cattgacatc cgcaacagct
     7921 atcatgtaga accgatattt catcagggcc tgcccg
//
LOCUS       DAOSDD010000210         7950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3365_length_7950_cov_5.042179, whole genome shotgun sequence.
ACCESSION   DAOSDD010000210 DAOSDD010000000
VERSION     DAOSDD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3365_length_7950_cov_5.042179"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..936)
                     /locus_tag="PK733_15295"
     CDS             complement(136..936)
                     /locus_tag="PK733_15295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11938.1"
                     /translation="MYNEIMYDTLARKISSFEIFDAHGHYGSDNRVHIPYYSAEDMIQ
                     TMNLAGVKKLFISSLIGISNNAYLGNTMVGDAIAKYPDRLIGMASIDPHKPSQIEKEL
                     KRCFGVLGMKMIKLHPSFSECPMVSRGYQSVYEFANERGIPILNHSWEDIHIVERILN
                     KYPNVVLIQAHSGGSWNGRSKNDFIDAAKEYPNFFVDTSYTIAYFGAFEKLVDYIGAD
                     KIIFGSDCPYMDLTYQLGKILFADISIKDKTKILSGNIRKIIEENSPI"
     gene            complement(929..1702)
                     /locus_tag="PK733_15300"
     CDS             complement(929..1702)
                     /locus_tag="PK733_15300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ11939.1"
                     /translation="MENTLFDCNCMYGKWNENTGISFINDNGLKVYMYKYGISDVLVY
                     HSIAKYNCVITGNSLLVKELGKIEGIHICWTLLPSSMEGFTDIFTIKRAFAANQVRAV
                     RLFPKLHEYLFKRWIVDDMLGMLNEINMPVILDFNNRLWHEQPDWDDIYSVCSGFPKI
                     PFILTRCSIASDKYLYSILSKLPNIYVETSFYQVNRGIEKLVKQFSAKRLLFGTGAPV
                     FNPGCPITMLELADISSEDKTRIRRLNMEELIKGVKCIV"
     gene            complement(1850..2617)
                     /locus_tag="PK733_15305"
     CDS             complement(1850..2617)
                     /locus_tag="PK733_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ11940.1"
                     /translation="MRIGCCTSIMNIKIAEKIGFDYIEPSVSKIAAMDEQSFKSSCNM
                     VDESSIKCESFNILFPKGMRLVGQDVYKEEVETYLESAFTRIKRFGARIVVFGSGGAR
                     KCPEGWDYEKAIQQLVEIARITGKIAEMFDIDIVIEPLNKQESNIINSVFEGINFVKK
                     VNHPRIKLVADFYHMRKENENMGVITNAAPFLHHIHIANSNGRSFPLDSFEDIYKEFF
                     EKLKNICYEGRISIEAKTDDFENEGIKALKLLRSLAV"
     gene            complement(2663..3931)
                     /locus_tag="PK733_15310"
     CDS             complement(2663..3931)
                     /locus_tag="PK733_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ11941.1"
                     /translation="MNLAINGGQKSVTSEAKEMWKPPYEIITSSISKMIYDGRLSGDW
                     EHTYFPFEREIANYFGAKYCAALNCGTSALWSAYIAIGIKPGDEVLCPTYTWISTIAP
                     ALFMGAAPILCDTEADTVLVDPEDVKKKITNKTKAIVVTHMYGNIVNMDAIMDISNKL
                     GIPVIEDCSHAHGAEWRGKKIGTIGHVGCFSMQGGYNGKPVPAGEGGFVLTNDRDIFN
                     KIIMYTHINRSNNPEDELYPEIIERYGAERFGMKFRAHPFSLVIAREMLKSLDYRNEQ
                     KSKYRNKIISTIGNMKGIKPFKQYEQAKTAGYFGGMQLLYDEKHLLGIPLYKIHKAIA
                     AEGVSLSYRNYKPLHLYQFLQDGHDLPGINMGIASQNYKGYTRNDFPTTNHIIEHLIG
                     MPVFIEETEGYSDQVINAFEKVLSNLNEIK"
     gene            complement(4119..5003)
                     /locus_tag="PK733_15315"
     CDS             complement(4119..5003)
                     /locus_tag="PK733_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11942.1"
                     /translation="MTSFIESIGERIFNIFNVLLMLMLAFATLYPMLYVAFASVSDPH
                     ALMKHAGLLFMPLGFCIDAYRAVFKNPMILRGYLNTLFIVIVGVGINIIFTSMGAYFL
                     SRKNIMLKNGIMFLIAFTMFFSGGMIPFYLTVMDLNIQNTLWSLILPVAINTYNLIIM
                     RTAFQNIPSSLEESAKIDGAGHFTILFRIIFPISLPVIAVMILYYGVEHWNSWFNAML
                     FLRNRELMPLQLILREIIIMNNTENMHLSVGVTDVVPVSETIKYAVIIVATIPILTLY
                     PFLQKYFVKGVLIGALKG"
     gene            complement(5013..5978)
                     /locus_tag="PK733_15320"
     CDS             complement(5013..5978)
                     /locus_tag="PK733_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11943.1"
                     /translation="MKPNLNTPEIASNHINKNRSYLHRVLKNMQKEKSLYVMIIPVLA
                     YYLVFHYKPLYGAIIAFKEFSPGKGIRLSPWVGFKHFTDFFKSMFFGRVLKNTITISL
                     SSIIFGFPAPIILALLINELKSKYFSRAIQTISYMPHFISMVVVCGLIRVFTKNDGII
                     TNFLYYFGFERVSLLENANLFVPIYVISGIWQEIGWGSIIYLAALTGIDAEMYEASRI
                     DGSGRWKQTLYITLPSLMPTIIIMLILRLGRIMSVGFEKVILLYNPLTYVTADVISSY
                     VYRKGILEFNWSFSSAVGLFNSVINFLFIVMANWISRKVNESSLW"
     gene            complement(6222..7856)
                     /locus_tag="PK733_15325"
     CDS             complement(6222..7856)
                     /locus_tag="PK733_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ11944.1"
                     /translation="MNRIFKIISFIMIVLIIATSLVACVKDHNKKTSPTTVNTTNMSQ
                     AEIENTYPINTEVTLSYWTPLNSKVSANYASLGDTPFAKELEKRTGVKINYMHSLIGN
                     ESEALNLMIASMDLPDIIDADMSYFSGGPETLIGDNIILKLNDIIDQYAPNFKKILQI
                     DAELDKMVRTDESSYFGFPYITDTEEDLCWRGPGIRKDWLDELGLQLPETIEEWYTAL
                     KAFKEKKNVIAPFSWNGSGTNALRAFSEGSFIGAYGVYLGFYQENGKVKYGPYEKEYK
                     DFLATMAKWYKEKLIDQNFASTDKKGLDANIINGNSGATILNRGGGFTLYIPALEKAD
                     PKSDLQPTAFPVLNKGDKPKFGHWLQKYRNIAAVITSKCRNVKIAAKWLDYGYTEEGS
                     MLNNYGIENVSYTIKNGIPKFTDEVLKNPDGKPVDESLAQYARIVYSGPFATRSMYNQ
                     LNIYEAQRNADLMWPKTDMVKHVMPPVTHSKNEGTQYSKIMNDVDTYLQEYSVKVIMG
                     IESLNNFDAYIGQLKNTGIEDAISIQQTALDRYKRR"
BASE COUNT         2491 a         1549 c         1146 g         2764 t
ORIGIN      
        1 gcccagctac agcacctcca cttatgcccc ttaccaatat tttcttcaga taaatcaaat
       61 gcacccgttg gaagcatgtg gctatagtca tgtgaggttt acaacgtcgt cgattaatga
      121 ttttatcctg gtgttctata ttggagaatt ctcttcgatt attttcctta tattacctga
      181 aagaatttta gttttatcct taatacttat gtctgcaaac aatatttttc ccaactggta
      241 agtaagatcc atatagggac aatctgatcc aaaaattatt ttatcggcac cgatataatc
      301 tacaagcttt tcaaaagcgc cgaagtaagc gattgtatat gaagtgtcca caaaaaaatt
      361 tgggtattcc tttgctgcat caataaaatc atttttactc ctgccattcc agcttcctcc
      421 agaatgcgct tgtatgagta caacattagg atatttattt agtattcttt caacaatatg
      481 aatgtcttcc caggaatggt tcaagatagg tatccctctt tcattggcaa attcatacac
      541 tgattggtaa cctcgactta ccatcggaca ttcagaaaat gaagggtgca actttatcat
      601 tttcattcct aataccccaa agcacctttt aagttccttc tcaatttgac ttggtttatg
      661 aggatcaatt gaggccatgc cgatcaaacg gtcaggatat ttagcaatag catctcccac
      721 cattgtattc cctaaatatg cattattgct aataccaatt aatgaagaaa taaagagctt
      781 cttaactcct gctagattca ttgtttgaat catatcctct gcagagtagt aaggaatatg
      841 taccctatta tcactcccgt agtgtccgtg tgcatcaaat atttcgaagc tactaatttt
      901 tcttgctaaa gtatcataca ttatttcatt atacaatgca tttaaccccc tttatcagct
      961 cttccatatt tagccgtctt attcttgttt tgtcttcgct tgatatatca gctaattcga
     1021 gcatagttat agggcaacca ggattaaaaa ccggagcgcc ggttccaaaa agaagacgtt
     1081 ttgcagaaaa ttgctttaca agtttttcta ttcctctgtt tacttgataa aatgatgtct
     1141 ccacataaat attaggcagt tttgataaaa tactatacag atatttatca gatgctatac
     1201 tgcatcttgt caatataaat ggaattttag gaaatccact gcatactgaa tatatatcat
     1261 cccagtcagg ttgttcatgc cacaaacgat tattaaaatc aagaattact ggcatattta
     1321 tctcatttag cattcctaac atatcatcaa caatccaccg cttgaataag tattcatgaa
     1381 gtttggggaa taatcttacc gctcttacct ggtttgcagc aaatgccctc tttattgtaa
     1441 aaatatctgt gaacccttcc attgatgagg gtagcaaagt ccagcaaata tgtatgcctt
     1501 ctattttacc aagctccttg acaagcaagc tatttccagt tattacgcaa ttatattttg
     1561 caatggaatg ataaacaaga acatcactaa tcccgtattt atacatatac acttttaatc
     1621 cattgtcatt tatgaacgat atgccagtgt tctcattcca cttaccatac atacaattac
     1681 agtcaaacaa tgtattctcc atgatttatc ccctaccttc atgaatattt tcaccccaac
     1741 tccatcttga atacttcatt aattatcttg ttattttcat ttttaatatc ttatgcctaa
     1801 tattaactat tacctctttt taaatatttt ttgattgaat tgcttaattt caaactgcta
     1861 gacttctaag taatttaagt gctttaatac cttcattctc aaaatcgtcg gtcttagctt
     1921 ctatacttat acgtccttca taacaaatgt tttttagctt ttcaaaaaat tccttataaa
     1981 tatcctcgaa ggagtctaat ggaaagctcc tgccattgct gtttgcaata tgaatatgat
     2041 gaagaaaagg agcagcattt gtaattactc ccatgttttc attttctttg cgcatatgat
     2101 aaaaatctgc tactagctta atgcgtggat gatttacttt cttaacaaaa tttattcctt
     2161 caaaaacaga attaataata tttgactctt gtttatttaa aggctctatc acaatatcaa
     2221 tatcaaacat ttcagctatt ttaccagtta ttctggcaat ctcaaccaat tgttggattg
     2281 ctttttcata gtcccaacct tcaggacatt tccttgcacc gccgctgcca aagactacta
     2341 tcctggctcc aaatctcttt attctcgtaa atgcgctttc taaatatgtt tcgacttcct
     2401 ctttgtaaac atcctgccct accagcctca tacctttagg aaacaaaata ttaaaggatt
     2461 cgcactttat cgaactttca tctaccatat tacagcttga tttaaacgat tgttcatcca
     2521 ttgcagcgat tttgcttaca gatggttcga tatagtcaaa ccctatcttt tcagctattt
     2581 tgatgttcat tatagatgta cagcaaccaa ttctcattaa ttctacaccc ctgatccagt
     2641 ttcgttgata ttcatataat gattatttaa tttcattaag attggacaac actttttcaa
     2701 aagcattaat aacctggtca gaatatcctt ctgtttcttc aataaataca ggcatgccaa
     2761 tcaggtgttc gataatatga ttggtggttg gaaagtcatt tctggtatat cctttgtaat
     2821 tttgagatgc tatacccata tttattccgg gtaaatcatg accgtcctgc aaaaactggt
     2881 aaaggtgtaa tggcttataa ttacgatatg ataacgaaac tccttctgca gcaatagctt
     2941 tatgaatttt atataaggga attcccaaca ggtgtttttc atcatataat agctgcatac
     3001 ctccaaaata tccggctgtt tttgcttgtt catattgttt aaatggctta atccctttca
     3061 tatttccaat ggtactaata attttattcc gatatttcga tttttgttca ttcctataat
     3121 caagagattt tagcatttcc cgtgcaatta ccaaactaaa aggatgcgct ctaaatttca
     3181 ttccaaacct ttctgctcca tatctttcta ttatttctgg ataaagctca tcttctggat
     3241 tattagatct attaatatgt gtatacataa taattttatt aaaaatatca cgatcattag
     3301 taagaacaaa accaccttca cctgcgggaa ccggctttcc attatatcct ccctgcatac
     3361 taaagcatcc aacatgacca atagtaccta ttttcttacc ccgccattca gctccatgag
     3421 catgggaaca gtcttcgata acaggaatac ctaacttatt cgagatatcc attattgcat
     3481 ccatatttac tatatttcca tacatatgtg taacaacaat cgctttggtt ttatttgtta
     3541 ttttcttctt aacatcttcc ggatccacca atacagtgtc tgcctcagta tcacaaagta
     3601 taggagcagc gcccataaac aatgcgggcg caatggttga tatccatgta tatgttgggc
     3661 ataatacttc gtcgccaggt tttattccaa ttgcaatata tgccgaccac aaggctgaag
     3721 taccacagtt taaagctgca caatattttg ctccaaaata atttgctatt tctctttcaa
     3781 atgggaaata tgtatgttcc caatcccctg aaagacgacc atcatagatc atcttgctta
     3841 ttgatgatgt aattatctca tatggaggtt tccacatctc ttttgcttct gacgtaaccg
     3901 atttttgtcc gccattaatt gctaaattca taattatcaa tcctttctag taatgtaatg
     3961 ttaatctatt ctagttatat atagattcta atgttaatgg atgagtattg catcatgact
     4021 tccagagtta aattcaataa aacaatttct aaatactttc tgttaagtga atcattttgc
     4081 gtatatatta aggatattag aggtatatat cttattaatc agccctttag tgcacctatt
     4141 aatacgccct tcacaaagta cttttgaagg aaagggtata atgtaagaat tggtatggta
     4201 gctactatta tcactgcata ttttatagtc tcgctgacag gtactacatc agtgacacct
     4261 acgcttaaat gcatgttttc tgtattgttc ataataatta tttctctcaa tattagttgg
     4321 agtggcatca gttctctatt tcgcaaaaat aacatagcat taaaccatga gttccaatgt
     4381 tcaacaccat aatataatat cattacggca ataacgggta atgaaatagg gaaaattatt
     4441 ctaaataaaa ttgtgaaatg tcctgcacca tctatcttgg ctgattcttc caaacttgat
     4501 ggtatgtttt gaaaagcggt tctcattata ataagattgt aagtattgat tgcaacaggc
     4561 aatatcaatg accataatgt attctgtata tttaaatcca ttaccgtaag ataaaaagga
     4621 atcattccac cactaaaaaa catagtaaat gcaatcaaaa acatgatacc attttttagc
     4681 ataatatttt ttctagagag aaagtaagct cccatggaag tgaaaattat gtttatcccg
     4741 acaccaacaa ttactataaa cagtgtatta agatagcccc ttaaaatcat cggattttta
     4801 aatacagcac gatatgcatc tatgcagaaa cctagaggca tgaataataa accagcatgc
     4861 ttcattaaag catgtggatc gctgacagat gcaaaagcaa cataaagcat aggatacaag
     4921 gttgcaaatg ccagcataag catcagcaga acattaaata tattaaaaat acgctctcct
     4981 attgattcta taaagctagt cataatatct cctcaccaca agctggattc atttactttt
     5041 ctactgatcc agtttgccat tactataaat aaaaagttaa taacgctatt gaaaagacct
     5101 actgcagaac taaagctcca attgaattca agtataccct ttctgtatac ataggacgat
     5161 atgacatcgg cagttacata cgtcaacgga ttatataaca gaataacctt ttcaaatcca
     5221 acactcataa tgcgtcccaa tctaagaatt aacatgataa taatcgtagg catcaaagaa
     5281 ggtagtgtta tatataatgt ttgcttccac cttccagagc catcgattct tgatgcttcg
     5341 tacatttcag catcgatacc tgtaagtgct gccagataaa taatagaacc ccatcctatt
     5401 tcctgccaga tacccgatat tacataaatt ggtacaaaca aatttgcatt ttcaagaagt
     5461 gatactcttt caaatccgaa ataatataaa aaattagtta taattccatc attcttggta
     5521 aaaaccctga tcaatccaca aactacaacc attgagataa aatgaggcat atagcttatt
     5581 gtctgaattg ctcttgaaaa atacttgctc tttaattcat taattagtaa cgcaagtata
     5641 ataggagcag gaaatccaaa aataattgaa gataagctga tagtaattgt atttttcagc
     5701 actctcccga aaaacatgct tttgaagaaa tcggtaaaat gtttgaagcc aacccatggg
     5761 ctgagccgaa ttccctttcc aggtgagaat tccttgaagg ctataatagc tccgtacaaa
     5821 ggtttataat gaaatacaag gtaatatgct aataccggaa taatcataac atacaggctt
     5881 ttttccttct gcatattctt taatactctg tggagataag atctgttttt gttgatatgg
     5941 ttactggcaa tttccggagt attcaaattt ggcttcatgg ctctgtcctc atttctgcat
     6001 aataaattaa ggtttggggc attgtgccca ctcgcttttg gtcaggtttg ctttgcaaat
     6061 gcccctggca aaaacatgtc atacttgaga attttttatc tttagtatta ccgcttttct
     6121 ttatattatg gaacttaaag gatataacct tttttaagga ttgtgtatat cccataagtc
     6181 ccatataaat aatataatta taaataatac gatttttaag tttatctgcg cttataccta
     6241 tcaagtgcag tttgttgtat agagattgca tcttcaatac cggtattctt tagttgtccg
     6301 atgtaagcat caaagttgtt aagtgattct atacccatta tgacttttac agaatactct
     6361 tgcaaatatg tatcgacatc attcataatc tttgaatact gtgtaccttc atttttcgaa
     6421 tgtgtcacag gaggcataac atgtttaacc atatctgttt taggccacat aagatcagca
     6481 ttcctctgtg cttcatatat attgagctga ttatacatac tccttgttgc aaacggacca
     6541 gaatacacta ttctggcata ttgtgccagt gactcgtcta ccggtttacc atcaggattt
     6601 ttaagcactt catcggtgaa tttaggtata ccatttttta ttgtatatga aacattttca
     6661 atgccataat tgttgagcat agatccttct tcagtgtagc cgtaatccag ccattttgca
     6721 gctatcttaa cattcctgca ttttgatgtg ataacagctg caatatttct atatttttga
     6781 agccaatgtc caaacttagg cttatctcct ttattaagca ctggaaaagc cgtaggttgt
     6841 aaatctgatt tcggatctgc tttttctaat gccggaatat ataaagtaaa tccgccgcct
     6901 ctattaagaa ttgtagctcc tgaattacca ttaattatgt ttgcatcaag ccctttctta
     6961 tcagttgagg cgaagttctg atcaatcaat ttttccttgt accattttgc catggttgca
     7021 agaaaatcct tatattcctt ttcatatggc ccatatttaa cttttccatt ttcctgataa
     7081 aaacccaaat atacaccata agcacctata aaagaccctt cactaaatgc tcttaatgca
     7141 tttgtcccgg agccgttcca ggagaaagga gctattacat tctttttctc tttaaaagcc
     7201 tttaaagcag tataccattc ctctattgtt tcaggtaatt gcagccctaa ttcatcaagc
     7261 caatccttcc ttattccagg tcctctccag cacagatctt cttcagtatc agttatataa
     7321 gggaatccaa aatacgatga ttcgtcagtt ctaaccattt tatcaagttc agcatcaatc
     7381 tgaagtattt ttttaaaatt tggtgcatat tgatcaataa tgtcatttaa tttaagaata
     7441 atattatcac caattagtgt ttcaggtccg ccagaaaaat aactcatatc tgcatcgatt
     7501 atatcaggta aatccattga agcaatcata aggtttaaag cttcactttc attacctata
     7561 agagaatgca tgtaattaat ttttacacca gttcttttct caagttcttt tgcaaaaggt
     7621 gtatctccta aactcgcata atttgcactt acttttgaat ttaatggtgt ccaatacgat
     7681 aacgtaactt ctgtattaat gggataggtg ttttcgattt ctgcctgtga catattagta
     7741 gtattaacag tagttggaga tgtcttttta ttatgatcct ttacacatgc aacaagagat
     7801 gttgcaataa ttaaaacaat cataataaaa gaaattattt taaagatcct attcattatt
     7861 actacctcct caattttcgt ttcaaaagag caccctttaa ttattatttt aggcaacttt
     7921 atataagatg tgaagtgaca atattagaaa
//
LOCUS       DAOSDD010000211         7824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3428_length_7824_cov_4.913374, whole genome shotgun sequence.
ACCESSION   DAOSDD010000211 DAOSDD010000000
VERSION     DAOSDD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_7824_cov_4.913374"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..2066
                     /locus_tag="PK733_15330"
     CDS             189..2066
                     /locus_tag="PK733_15330"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ11945.1"
                     /translation="MFTDKLKERFSNNFIFNMNIRNRLLIFFLIIVIFPTSIIIVAVY
                     KRSIGIITQRIDSSLKDNLNMAEINIMQKFETINDISTFIYMNTDLVSILSVKYPPER
                     PSYVTSDRIDMINEMATLDKLLDNYKLSGITGMTIFPRIYMVNRPEYLQSNFSNKVSS
                     LSLIETEEWYLNLPASARYTVVGLNNEPLLTDSPGTIRVAKKLFGLKNPDIPYSALLT
                     IDISVADFTNILEKFRPSKNSNVYVIDGDSNIVINPDYSLLGEKLYKVKMYSRDINFQ
                     SEYASFKEKINNTEMLVTYKRISSLNWKIVAFCPVSELYGELISFRKIIYFILVLCIT
                     LTVLLAIFLSENLSYPIRKLIDSMASVKEGKFDISFEYRRNDEFSYLIDTYKNMLDEI
                     KQLIDQLYISEESKREAELKALQAQINPHFLYNTLDSVNWLALKYNADDISTMVTSLS
                     DFFRYTLSKGKNIIPLGDEKKQIESYLVIQKIRFVDKLDYNISFSRETLDYLTVKLIL
                     QPIVENSIIHGIEKKKGKGIIDIIAEKVEDNIEIKISDNGVGGNIEEMNSLLQNRGET
                     VKSYGIRNVNERIKQLFGEPYGIKFYSCKKAPINRETGINNGSGVTALITIPAVKKWR
                     V"
     gene            2067..3686
                     /locus_tag="PK733_15335"
     CDS             2067..3686
                     /locus_tag="PK733_15335"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ11946.1"
                     /translation="MIKMVIADDEYIVREGLKNNIHWEEFGIEIVGDACDGEEAFDLC
                     MKLQPDILFTDIRMPIMDGLEVAMRLKENKSSIKVIMLSGVQDFNYAKTALDVNAEGY
                     ILKPVKINELRSVIAKAVNSIKLEKCREHEIMSLKQELSNNVPIIRENFLRNLISSFY
                     QNEDEINEKLSYLEIPLKSDESVAVAVFQIDEYERITRNSTEEERQLLNLSVINIMKK
                     IIHEYGFGISFTAKENEFVVIYNQNAQLRKKYIDVCEEIMSCVNKFSGETVSVGIGRT
                     VSKPDDVAISYREAVDALEYKFYTGKGSMISIDDIYQDEDDIDFSDLYNLEQKLMNCM
                     RLGDTKSVSETLEAIFMPLLYSGKTHCYAYIKSVCIELVCTAQRVIYETGEDMKDIID
                     DYSIILSNIFIKKDMFELKSYVKSIFIQLVDFFAGRYNNKNAGVIRKIKSIIDERYME
                     NISVSMLSEEIYLTPNYISLIFKRETGETIVEYITRVRMEAAKELLKSSELKVFEIAG
                     MVGYENPNYFSTVFKKYTGVHPGKYQCIKNQ"
     gene            3983..4894
                     /locus_tag="PK733_15340"
     CDS             3983..4894
                     /locus_tag="PK733_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ11947.1"
                     /translation="MLLTDLSKNKAFLFMLLPGTIILILFSYMPMFGIVLAFKDFNLQ
                     KGFFGSDFVGLRNFKFLFATEDAYIITRNVVLYNLVFIFAGNVLAMFAAIAINELKNR
                     YAAKFYHSCMFLPYFLSWVVVSYLAYAFLNVERGFINTILVNTFNHEAIYWYNEPKYW
                     PFIIVGANFWKGTGYSSIVYLSAIMGIDKELYEAAEVDGATKWYQIRKITMPLLTAIM
                     SVLILMGLGGIFRGNFGLFYQLPLNSGSLFKVTNVIDTYVYRALMAMGDIGMSSAAGL
                     YQSVVGFILVLASNLAVRRMDESKSLF"
     gene            5051..5953
                     /locus_tag="PK733_15345"
     CDS             5051..5953
                     /locus_tag="PK733_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ11948.1"
                     /translation="MASKYNSISNAANIILNIFFIIYTIMCIGPLMLVLIISITDESM
                     LIQNGYSFFPQKLSFEAYRFIFKESGVLARAYLNTCLVTFAGTILVVMITAMFAYPLS
                     RKEFKYRKGFSFFLFFTMLFSGGVVPWYLVCVRILNLKNTIFSLFVPYLMSAWYVLIM
                     RTFFSTTIPDSLIDSARIDGAKEFRIFFSIVLPLSTPSLATIGLFSALMFWNDWWLPL
                     MLTTGDRYINLQYMLQRVQQDIEFITQMKEIGGGVIPELAGKIPSESSRMALCILAIG
                     PIIFIYPFLQKYFVQGLTIGAIKG"
     gene            6005..7519
                     /locus_tag="PK733_15350"
     CDS             6005..7519
                     /locus_tag="PK733_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ11949.1"
                     /translation="MSKFKKIFCIVLTLFIVLGLMSGCEITKKEPALKQDSGEDNAPK
                     VPDLKPYEINWYIPGSAQKDVALVEEAANNYLKDKINASLKLVFMTWADYASKVNVML
                     QSGEYFDICFTSDWSTPKYRTSVAAGLFTPLNDPLNNLLDQYAPKTKAVLGEDVLKGT
                     RINGLNYAIPCNKEMARCRGFLFNKELVDKYKFDLTTIKKYQDVEPMLKVIKENEPDI
                     YPNNKGGIPLDWNFITDTDVPLATYTKAGAKEVLFWYETPEYQELIRTVNKWWKLGYI
                     RQDFNTSKEDDVKNYKVFCADYTLKPGKDAEISNQYGNKWVQVMITPTIMSNVELGGS
                     MQAIPRSSKDPARVMMLLELINTDPYMNNLINFGIEDKHYVKVSDNIIDFAPGTDGGK
                     NSGYNPAMSWVVGNVFINYLFKTEDPRKAEKYLEFNKSAIVMETLGFSFNNTPVKTEI
                     AAIKNVFAEYNSIMALNPDENIPKAIAKFEAAGIRKVQEEAQKQLDEYLSKTKK"
     gene            7614..7799
                     /locus_tag="PK733_15355"
     CDS             7614..7799
                     /locus_tag="PK733_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11950.1"
                     /translation="MLYNNDNCIKDSYYIKGKSTETKNIKSVKKKRTYYTDKKINAAR
                     TNIGVYQWACACSVINI"
BASE COUNT         2771 a         1117 c         1508 g         2428 t
ORIGIN      
        1 aagttaataa aaaacgaact tttttattca aaaccatgta gtatatatga attttaacat
       61 tagcttgtat atataaatgt tatattgtac aatttaatta tatcaatatt atgtaaaatt
      121 gcatatatat atgaaactta ctgaacataa ttatatataa gtttatagtt taatggagtg
      181 aagataacat gttcacggat aagctaaaag agaggttttc aaataatttc atatttaaca
      241 tgaatataag gaacaggctt ttgatatttt ttctcattat agttatattt ccgacttcta
      301 ttataatagt ggctgtttat aagcgctcta tcggtataat cacacaacga attgattcct
      361 cactgaaaga taatcttaac atggcagaga taaacataat gcagaaattt gaaacaataa
      421 atgatatatc tacctttata tatatgaata ccgaccttgt cagcatactt tcagtaaaat
      481 atcccccaga aaggccttca tacgtgactt cagacagaat tgatatgata aatgagatgg
      541 caactcttga taaattgctg gataattaca agctttcagg aattacgggg atgaccattt
      601 tcccaagaat atacatggtt aacaggcctg agtatttaca gtcaaatttt tcaaataaag
      661 tatcaagctt aagtttgatt gaaactgaag agtggtacct taatttgcct gcatcagcca
      721 gatatactgt tgtaggactc aataatgagc cattattaac cgactcacct ggaacaataa
      781 gggtagcgaa gaagcttttt gggttaaaga atcccgatat accatatagt gcattattga
      841 ctatagatat tagtgtggca gattttacga atattcttga aaagttcaga ccttcaaaaa
      901 acagcaatgt atatgttata gatggagatt ccaatattgt aataaatccg gattattcat
      961 tgcttggcga aaaattatat aaagtaaaaa tgtatagcag ggatataaat ttccagtcag
     1021 agtatgcatc atttaaagaa aaaataaaca atactgaaat gcttgttaca tataaaagga
     1081 tcagcagtct taactggaaa atagttgcat tttgcccagt atccgaatta tatggagaat
     1141 taatatcttt caggaaaata atatacttta tacttgtact atgtataact cttactgtcc
     1201 ttcttgcaat ttttctttct gaaaacctct catatccaat acgcaagctt attgactcaa
     1261 tggcatcggt aaaggaaggt aaatttgaca tatctttcga atatcggcgg aacgatgagt
     1321 tttcatatct gatagataca tataagaata tgcttgatga aataaaacag ttaatagacc
     1381 aactctatat aagtgaggaa agcaaacggg aagcagagtt aaaagcattg caggctcaaa
     1441 taaatccaca ttttctgtat aatactttgg actcggtaaa ttggcttgca ctaaagtata
     1501 atgcagatga tatcagcaca atggttactt ccttatcgga tttttttcgt tataccctca
     1561 gcaaggggaa aaacataatt cctttgggtg atgaaaaaaa acagatagaa agctacctgg
     1621 taatacaaaa aattaggttt gttgataaac tagattataa catcagtttt tcccgggaaa
     1681 cacttgacta cctcactgta aagcttattc tgcaaccaat agtagagaat tctattattc
     1741 acgggattga aaagaagaag ggaaagggga ttattgatat tatagctgaa aaagttgaag
     1801 ataatattga aataaaaata tcagataatg gtgtaggtgg aaatattgag gagatgaact
     1861 ctcttcttca aaatagagga gagacggtaa aatcttatgg aataagaaat gtaaatgaaa
     1921 ggataaaaca gttatttggt gagccatatg gtataaagtt ctactcttgc aaaaaagcac
     1981 caataaacag ggaaaccggt ataaacaatg ggtcaggagt tactgcattg attacgatac
     2041 ctgcagtgaa aaaatggagg gtttagatga taaaaatggt aatagccgac gatgaatata
     2101 tcgtcagaga agggcttaaa aataatatac attgggaaga attcggcatt gaaattgtag
     2161 gagatgcttg tgatggcgaa gaagcgtttg atctctgtat gaagcttcag ccagatatac
     2221 tttttactga tattagaatg cccataatgg atggtcttga ggttgccatg agactaaaag
     2281 aaaataaaag cagcatcaag gttattatgt tgagtggtgt tcaggatttt aactatgcca
     2341 aaacggctct ggatgtaaat gctgagggat atatactgaa gcctgttaaa attaatgagt
     2401 tgcgaagtgt gatagcaaaa gctgtaaata gcataaaatt agaaaaatgt agagaacatg
     2461 aaataatgtc tttaaagcaa gaacttagca acaatgttcc gattattaga gaaaattttt
     2521 tgcgaaacct tatttcaagc ttttatcaaa atgaagatga aataaatgaa aagctcagtt
     2581 atctggaaat acccttgaaa tcagatgaaa gtgttgctgt tgccgtgttc caaattgatg
     2641 aatatgaacg tataaccagg aatagtactg aagaagaaag gcagctctta aacttgtcgg
     2701 ttattaatat tatgaaaaag ataatccatg agtatggctt cggaattagt tttactgcta
     2761 aagaaaatga atttgtggtt atatataacc aaaatgctca attgaggaaa aaatatatcg
     2821 atgtatgtga agaaataatg tcatgtgtta ataaattttc aggtgaaact gtatcagtag
     2881 gaataggacg cactgtaagc aaaccggatg acgttgcaat ttcatacaga gaggctgtag
     2941 atgcattaga atacaagttt tatacaggaa aaggttctat gataagtata gatgacatct
     3001 atcaggatga agatgatatt gacttctcgg atctgtataa tttggaacaa aaattaatga
     3061 attgtatgag gcttggggat acaaaaagtg tatcagaaac cctagaggct atatttatgc
     3121 cgcttctata ttctggtaaa acgcattgct atgcatatat caaaagtgta tgtattgaac
     3181 tggtatgcac tgcacaaagg gttatttatg aaactggaga agatatgaag gatattatag
     3241 acgactactc cataatttta agtaatatat ttataaagaa agatatgttt gaactaaaaa
     3301 gctatgtcaa atctatattc attcaattgg tagatttttt tgcaggaagg tataataaca
     3361 agaatgcagg agttataaga aagattaaaa gtattattga tgaaagatac atggagaata
     3421 taagtgtatc catgctgtcg gaagaaatat atcttacacc taactatata agtcttatat
     3481 tcaaaagaga aacaggcgaa acaattgtgg aatatataac aagagttcgt atggaagcag
     3541 ctaaagagct tcttaaatct tcagaattaa aggttttcga aatcgcagga atggtagggt
     3601 atgaaaaccc taattatttt agtactgttt tcaaaaagta tacaggagtt caccctggta
     3661 aatatcaatg tatcaagaat caatagaaaa ttatactatt ttatagatta tttcatttta
     3721 ataatttcat atatcactta taatataata aagatagcga attatattgt ttaacccaag
     3781 cattaagaag tctctcgctt ctacaagctg gagataaact gtataaaaca aatagtagta
     3841 taaaaatata caatgcataa atatcacata aaacatataa atgaaatctc atattgcagt
     3901 ataatttctg cagcggaagg ggctttatat gaaatctggt attttatcga agcaaagaac
     3961 cggcagtaac aaaataggaa atatgctgtt gactgattta tcaaagaata aggcattttt
     4021 atttatgctg cttccgggaa caataattct gatactgttt agttatatgc ctatgtttgg
     4081 tatagtactg gcatttaaag atttcaattt acaaaaaggt ttttttggaa gtgactttgt
     4141 aggtcttaga aattttaaat ttttgtttgc aacagaagac gcatatataa ttacgcggaa
     4201 tgttgtacta tataatttag tctttatttt cgcagggaat gtattggcaa tgtttgctgc
     4261 aatagctata aacgaattaa aaaaccgcta tgccgcaaag ttttaccaca gctgcatgtt
     4321 cctgccttac tttctttcgt gggtggtagt aagttattta gcctatgctt ttcttaatgt
     4381 agaaagagga tttataaata ctattcttgt gaatactttt aatcatgagg caatttattg
     4441 gtataacgaa cctaaatatt ggccttttat tatcgtggga gctaatttct ggaaagggac
     4501 aggctacagc agcatagtat atctttcagc aataatgggt attgacaagg agttatatga
     4561 agcagctgaa gtagatggcg ctaccaagtg gtaccagata agaaaaatta ccatgcctct
     4621 gcttactgct ataatgagtg ttttgatact tatgggattg ggaggaatat tccgggggaa
     4681 ttttggtctc ttttaccagc ttcctcttaa ctcaggttca ctttttaaag ttacaaatgt
     4741 aatagataca tatgtttata gagcattaat ggctatgggt gatataggga tgtcctctgc
     4801 agcagggtta taccagtcag ttgtcggttt tattctggta ctggcttcaa accttgctgt
     4861 aagaagaatg gatgagagca aatcattatt ctgataaatt aacacaaata tctaaaaatt
     4921 attattcaaa ctttttgact attaaggtac ttattaatgt gctaaatcag ctttcttaaa
     4981 aacctgattg accagtttat tttatgatta tgtttaaaat aattttatta attaacaggg
     5041 ggctcacatt atggcaagta aatataattc aatctccaat gcagcaaata taatattaaa
     5101 tattttcttt attatttata caataatgtg cataggtcct cttatgcttg tgttaataat
     5161 atccataaca gatgaaagca tgcttattca aaatggatac agcttctttc cgcaaaaatt
     5221 gagttttgag gcatacaggt ttatattcaa ggaatcagga gtgcttgcca gagcttattt
     5281 aaatacttgt ctggtaacat ttgcaggtac tattttagtt gttatgatta ctgccatgtt
     5341 tgcctaccca ttgtccagaa aggagtttaa gtatcggaag ggtttttcat ttttcctgtt
     5401 ttttactatg ttgttcagtg gcggtgtggt gccatggtat ttggtttgtg tgagaatttt
     5461 aaatttaaaa aacacaattt tttccctttt tgttccttat cttatgagcg catggtatgt
     5521 gttgataatg aggacgtttt tttcaactac aataccggat agtcttatag attctgcaag
     5581 gatagatggg gcaaaagagt tcaggatttt ctttagtatt gttttacctc tttccactcc
     5641 ttcgcttgca actataggcc tatttagtgc tcttatgttc tggaatgatt ggtggcttcc
     5701 tttaatgctt acaaccggcg atagatatat aaatcttcaa tacatgctgc aaagagtcca
     5761 gcaggatata gagttcatta cacaaatgaa ggaaataggc ggaggtgtta ttcccgagtt
     5821 agctggtaag ataccaagtg aatccagccg tatggcatta tgtatacttg caattggacc
     5881 aattattttt atatacccgt ttctgcaaaa atatttcgta cagggactta ctattggagc
     5941 tattaaaggc taatactatt ttggtagatt agcatatata aaaaataaac aaggaggtct
     6001 tttcatgagt aaatttaaaa aaatcttttg catcgtgtta acattattta ttgtactcgg
     6061 gttaatgtca ggatgtgaga taaccaaaaa agagccggca ttaaagcagg atagcggaga
     6121 ggataatgcc cccaaggtac cggatttaaa accatatgaa ataaactggt atatccccgg
     6181 gagtgctcaa aaggatgttg cactggttga agaagcagcg aacaattatc taaaagataa
     6241 aattaatgct tcgctaaagc ttgtttttat gacttgggcg gattatgctt caaaggtaaa
     6301 tgttatgctt cagtcgggag aatactttga catctgcttt accagcgact ggtctacacc
     6361 taaatataga acaagtgttg cagcagggct gtttacgcct ctaaatgatc ctctgaacaa
     6421 tttattagat caatatgcac caaaaacaaa agctgttctg ggagaggatg ttcttaaagg
     6481 tacaagaata aacggactta actatgccat accctgtaat aaggaaatgg cacgctgcag
     6541 aggcttcctt ttcaacaagg aactggtaga taagtataaa ttcgatctta ctacaataaa
     6601 gaaatatcag gatgttgaac ctatgctgaa agtaattaag gaaaatgaac ctgatatata
     6661 tcctaataat aagggaggaa taccactcga ctggaacttt attacagata cagatgtacc
     6721 ccttgctacc tatacaaaag caggagctaa ggaagtatta ttctggtatg agactcctga
     6781 atatcaggaa ctgatcagga cagttaacaa atggtggaaa ctcggatata tcaggcagga
     6841 ctttaataca tcaaaggaag acgatgttaa aaattataag gtgttttgtg cagattatac
     6901 tttaaaacct ggtaaagatg cggaaatatc caatcagtat ggcaacaaat gggttcaggt
     6961 catgattact cccaccataa tgtcaaatgt agaacttgga ggctcaatgc aagcaatacc
     7021 cagatcgtca aaagatccgg caagggttat gatgttacta gaactgatca acactgatcc
     7081 atatatgaat aatctaataa actttggtat tgaggataaa cactatgtaa aagtatctga
     7141 caacattata gattttgcac cgggtacgga tggtggaaaa aattccgggt ataatcctgc
     7201 tatgtcatgg gtggtcggta atgtgtttat aaattacctt tttaagactg aagacccaag
     7261 gaaagctgag aagtatctgg aatttaacaa gagtgctata gtaatggaga ctcttggatt
     7321 cagcttcaat aatacacctg ttaaaacaga aatagcggca atcaaaaatg tatttgcaga
     7381 gtataattcg attatggcac ttaaccctga tgaaaatatt ccaaaggcaa ttgccaaatt
     7441 cgaggcagct ggaattcgca aggttcagga agaggctcaa aaacagcttg atgaatacct
     7501 ttcaaaaact aaaaaataga agcatgtaaa aatgatgcag cgggtgatgc aacgggaacg
     7561 gttccatctc cgctgcattc ttgcctggaa atcctttaga tggaggagac aatatgcttt
     7621 acaataatga taattgcatt aaagatagtt attatattaa aggtaaaagc actgaaacaa
     7681 aaaacattaa aagcgtaaaa aagaaaagaa catattatac agataaaaaa attaatgcgg
     7741 cacgaacgaa tataggcgtg taccaatggg cgtgcgcctg ttcggtgatt aatatatagt
     7801 gggtgcgaat cccatctggt aaag
//
LOCUS       DAOSDD010000212         7740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3479_length_7740_cov_4.939232, whole genome shotgun sequence.
ACCESSION   DAOSDD010000212 DAOSDD010000000
VERSION     DAOSDD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3479_length_7740_cov_4.939232"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PK733_15360"
     CDS             complement(<1..849)
                     /locus_tag="PK733_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HOJ11951.1"
                     /translation="MKITFPHMGNLYICVKALLDDLNIDYIIPPFSSKKSLELGTRFA
                     PECACLPLKITIGNFLEANEQGADTVLMAGGCGPCRFGYYCEMHREILADLDVNMNVI
                     ALEPPDGDIKELLKRIRELTGGFNVFKIFNAVKNATKIAIAVDELERLTFKIQPREIE
                     KGSVNSIYKQFRENVVKTRSSREIKELIKNTREKLSHVKINDKANPLRIGIVGEIYTT
                     IDDYTSFDIQQRLGRMGVEVDRYVTVSGWITEHIMKQALKLPRDLSYAKAAKPYLGTM
                     IGGHAQE"
     gene            complement(803..1870)
                     /locus_tag="PK733_15365"
     CDS             complement(803..1870)
                     /locus_tag="PK733_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOJ11952.1"
                     /translation="MNKTNIKVGIPRGLFYYQFYPLWKSFFEELGAEIVLSDNTTRKI
                     LDDGVKKCIDEACLPVKIFHGHVASLKGKVDYLFIPRYTSISKKEYICPKFGGLPDMI
                     RHTFDELPKIIDTEINLRKSNRNALKAAIEIGRYFSDDTDSIKKAYRKAMANYREFRQ
                     NVKNSTIPVELLGKKTAGAETQSKEEGSNRTLSNENQSNEAPLNIAILGHGYNLYDNY
                     ANMNLLAKLKELGSRITTVDMVDGRVIQEKSKLLNKKTFWYFGTKVLGSTFHLLDRDD
                     IDGIIYIMSFGCGIDSFICDLVERKVRRTGSIPFIIITIDEHSGEAGLNTRIEAFIDM
                     IRWRNNYENNISSYGQPVYLR"
     gene            complement(2030..2959)
                     /gene="fba"
                     /locus_tag="PK733_15370"
     CDS             complement(2030..2959)
                     /gene="fba"
                     /locus_tag="PK733_15370"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOJ11953.1"
                     /translation="MPLVTSKEMFKKAYEGGYAIGAFNVNNMEIIQGITEAAKEVNAP
                     LILQVSAGARKYAKHVYLMKLVEAALEDTGLPICLHLDHGEDFEICKSCVDGGFTSVM
                     IDGSKHPFEENIRLTKQVVDYAHSKGVVVEGELGKLAGIEDAVNVSEKDAAFTDPDQV
                     QEFVEATGVDSLAIAIGTSHGAYKFKGEAKLRFDILEEVQRRLPSFPIVLHGASSVIP
                     AFVDDINKYGGKMPGAKGVPEDMLRKAASMAVCKINIDSDLRLAMTAAIRKHFGDHPE
                     DFDPRKYLGPARSSIKEIVKHKLINVLGCAGKA"
     gene            complement(3381..4514)
                     /locus_tag="PK733_15375"
     CDS             complement(3381..4514)
                     /locus_tag="PK733_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11954.1"
                     /translation="MKRYISIILSILVITTFCTLGVVAESVATGTTDEVQQAVSGQEE
                     LQDEEIDNSANVLEKLLVSIKIDTVILEDGNTELAVSFQNEKLDDERIVTEEDLQGLL
                     EKMSGIFLEIANINSSSDEGTEIGPDDIAASIEEIKNDDGQVTGFILKIGGKVSEECS
                     DSIKNGIENQVIRTLADCTYEMVKDNLEEASDLAIQALRELILAKESGDQALVDAAKA
                     KFDAAIQMEDSLKALKNEIELYREQILDIIDDYEKDEEDEEEDEEDEDDKELKDEEKL
                     KTMAERIKNLIKKHYEKRQQLAEKLKEHSENAAEMVEKGTNGQIERKIEKLNKIVNGE
                     EEEDEEDDEDEVDDEDEEDEEDEEDEDDGESAARGKLLNKRKK"
     gene            4905..6152
                     /locus_tag="PK733_15380"
     CDS             4905..6152
                     /locus_tag="PK733_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ11955.1"
                     /translation="MTQLKGACIFGQSGGPTSVINASASGVFQEALKQDCITAVYGAA
                     HGIKGILDEVFYDMSKEDQYELELLKTTPSSALGSVRYRLKKAEDDETDYKRILEVFK
                     KYNIRYFFYNGGNDSMDTCNKISKYMQKAGYECKVMGVPKTIDNDLWGTDHCPGYGSA
                     AKYIATSTMEIYHDARVYDKGQITVLEIMGRNAGWLTAASALAAYKGHGPDLIYLPEL
                     PFDVDRFIDDVTKIYKANGNAIVAVSEGIKDKNGKYISEYGSDLAQSKDAFGHAQLGG
                     LASTLANFAKENTGAKVRGIEFSLLQRCAAHLGSLTDVNEAFMAGQAAVQYAVQGITD
                     KMVAFERAEGTEYKCSIKLIDLTDVANTEKKVPFEWIINNGTGISDEYIKYALPLIQG
                     ESTPPVEDGLPRFAKLKKVLAAK"
     gene            6437..6625
                     /locus_tag="PK733_15385"
     CDS             6437..6625
                     /locus_tag="PK733_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ11956.1"
                     /translation="MADKTLICKDCNSEFVFTEGEQAFYKEKGFQNEPQRCPECRKAR
                     KQQRRNNSGSHKFGDNRM"
     gene            6814..7740
                     /locus_tag="PK733_15390"
                     /pseudo
     CDS             6814..7740
                     /locus_tag="PK733_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT         2343 a         1495 c         1242 g         2660 t
ORIGIN      
        1 ttcctgggca tgtcctccaa tcatggttcc aagatatggc ttcgctgctt ttgcataact
       61 taagtctctt ggaagcttaa gagcctgctt cataatatgt tctgttatcc atccgcttac
      121 cgttacgtag cggtccactt caacgcccat tctgccaagc ctttgctgaa tatcaaaact
      181 tgtgtagtcg tcaatcgttg tatatatctc tcctactata ccaattctta acggattggc
      241 tttatcattg atttttacat gagataactt ttctctggta ttctttataa gctcctttat
      301 ttcacgggaa ctccttgttt ttacaacgtt ttctctgaac tgcttatata tactattaac
      361 agagcctttt tcaatctccc ggggctgtat tttaaatgtt agtctttcca gttcatctac
      421 tgctattgct atcttagtag cattttttac agcgttgaaa attttaaaga cattaaatcc
      481 acctgttagt tctctgatcc tcttaagaag ttctttaata tccccgtctg gaggttccag
      541 agcaataaca ttcatattta cgtctaaatc agccaatatc tccctgtgca tttcacagta
      601 atagccaaat ctgcagggtc cacatccccc tgccattaat acagtatcag ccccctgttc
      661 attagcttcc aaaaaattgc ctattgttat tttcaatgga aggcatgcac attcaggtgc
      721 aaatcttgta cctagttcca atgatttttt actgctaaaa ggggggatta tataatcaat
      781 atttaaatca tccaataatg ctttaacgca aatatacagg ttgcccatat gaggaaatgt
      841 tattttcata attgtttctc catcttatca tatcaatgaa agcttctatt cttgtattta
      901 ggcctgcttc gcccgaatgc tcatcaatgg taattattat aaaggggatg ctcccggttc
      961 tacgtacctt tctttcaaca agatcacata tgaaagaatc tatcccgcat ccaaaactca
     1021 taatatatat tattccatct atatcatctc tatccagcag gtggaatgta cttccaagta
     1081 ctttcgtacc aaagtaccag aatgtttttt tatttaatag ctttgatttc tcctgtatga
     1141 ctcttccatc aaccatatct actgtagtaa tgcgtgatcc taattctttc agttttgcta
     1201 ataaattcat gtttgcataa ttatcatata agttgtatcc atgacccaga atagcaatat
     1261 ttagtggggc ttcattgctt tggttttcgt tgctcaatgt tcgattgctg ccttcttctt
     1321 tgctttgtgt ttcagctcct gctgttttct tgccaagcag ctcaacagga atggtgctat
     1381 ttttaacatt ctgtctgaac tctctataat ttgccatggc tttcctatat gcttttttta
     1441 tcgaatctgt gtcatcagaa aaatatctgc caatctctat tgctgctttt agagcatttc
     1501 tattagattt tctcaggttt atctctgtat ctattatctt cggaagctca tcaaaagtat
     1561 gccttatcat gtcaggaagg cctccaaact taggacatat atactccttt ttggaaatac
     1621 tcgtatacct tggtataaat aaatagtcta ctttgccttt aagacttgca acatgcccgt
     1681 ggaagatttt aaccggcaga catgcttcat ctatacattt ctttactcca tcatcaagaa
     1741 tttttcttgt tgtattgtct gataaaacta tttcagctcc cagctcttca aaaaagcttt
     1801 tccacagagg atagaactga tagtaaaata atcctctggg aatgccaact tttatatttg
     1861 ttttgttcat ctgtcaacac ctcgaaaaat agtattgact tatgatattt gatttatgca
     1921 gtataatttt taatagaaag tacataaaac aaaaagggct gtccttaatt cagaataaat
     1981 cctcagaaca gtccttttta ataacaatat tatttgtaat tcttaaatat taagctttgc
     2041 ctgcacaacc caatacatta atcaatttgt gcttaactat ctcctttata gaacttcttg
     2101 caggtcccag gtattttctt ggatcaaaat cctcaggatg atcaccaaaa tgctttctaa
     2161 tagctgctgt catggcaaga cgcaggtctg aatcaatatt gattttgcat acagccatgg
     2221 aagctgcctt tctgagcatg tcttcaggta cccccttagc tccgggcatt ttaccaccat
     2281 atttattgat gtcatcaaca aatgccggaa taactgatga agcaccatgg agtactattg
     2341 ggaaactggg aagccttctt tgtacttcct ccagaatatc aaaccttaat tttgcttctc
     2401 ccttaaactt atacgcacca tggcttgtgc ctatagcaat agcaagtgag tcaacaccgg
     2461 tagcttctac gaattcctga acctgatccg gatcagtaaa agcagcatct ttctccgata
     2521 cattgactgc atcttcaata cctgctaatt tccccagttc gccttcaact acaacacctt
     2581 tgctatgagc ataatctaca acctgtttgg ttaatcttat gttctcttca aaaggatgct
     2641 tggaaccatc aatcatgaca gaagtaaaac caccatcaac acatgattta catatttcaa
     2701 aatcttctcc gtggtcaaga tggagacaaa taggaagacc tgtgtcctcc agtgctgctt
     2761 caacaagttt cattaagtac acatgttttg catattttct tgctcctgct gaaacctgca
     2821 gtataagagg agcattaacc tctttggctg cttcggtaat tccctgaata atttccatat
     2881 tattaacatt gaatgcacct attgcataac cgccttcata agcttttttg aacatttcct
     2941 ttgatgttac taatggcata tataatctct ccttttatgt tatatattta tataatatat
     3001 attactttaa ctattgtgcc aaatttatat tcggacacct gcattactgg taaagattct
     3061 gccagcacat tcagcaccaa cttaacagca aatattttca ggcatgtcct aaatatcagc
     3121 actaatagca acacgccggg aaaactatgt taagtttttg acaggtgttt agttataatt
     3181 ttaccaaatt gaataataaa atcaagtatt attttatact tagcaccacc agggttatgt
     3241 cgaattatgt cgtatacatt agatgtctta aactcagtgt tttcaataac aatcatgtac
     3301 ttgatattta aaaagggcct tacatggtta atgttcacca tgcggcccat aatttcggag
     3361 gttaaatcat ccttcttttt ttattttttc cttttgttta acaactttcc tctagctgca
     3421 ctctcaccat catcttcgtc ttcttcatct tcttcatctt cttcgtcttc atcgtctact
     3481 tcatcttcgt catcttcttc gtcttcctct tcctcaccat taactatctt attaagtttt
     3541 tctatttttc tttctatttg accatttgta cctttttcaa ccatctctgc agcattttcg
     3601 gaatgctcct tcaatttctc agctaattgc tgtctctttt catagtgctt cttaataaga
     3661 ttttttattc tttcagccat tgtttttagt ttttcttcat ctttaagttc tttgtcatcc
     3721 tcatcttctt cgtcttcttc ctcatcttct tcatctttct cataatcatc aattatatct
     3781 aatatttgtt ctctatacag ttctatctcg ttttttaaag ctttaagact atcttccatt
     3841 tgtattgctg catcgaactt tgctttagct gcatctacca aggcctggtc tccggattct
     3901 tttgcaagta tcagttctct tagtgcttgt attgccaagt ctgaagcttc ttctaaatta
     3961 tcttttacca tttcgtatgt acagtctgca agagtcctta ttacttggtt ttcaattcca
     4021 ttcttaatac tatcagagca ttcttcagaa actttaccac ctattttcag tataaaaccc
     4081 gttacctgac catcatcatt ttttatttcc tctatagaag ctgctatatc atcgggacct
     4141 atttcagtac cctcatcgct actactgttg atgttagcaa tctccaggaa aatacctgac
     4201 attttctcca acaacccttg taaatcttcc tccgtaacta ttctttcatc atcaagtttt
     4261 tcattctgaa aacttactgc tagttcggta tttccatctt ccaaaattac agtatcaatc
     4321 tttatactta caagaagctt ttccaaaaca ttcgctgagt tatctatttc ttcatcttgc
     4381 agttcttcct gcccgcttac agcttgctga acctcatctg ttgtaccggt tgcgacactc
     4441 tctgctacta cacctaatgt gcagaaagta gttattacca gtatcgatag aatgattgaa
     4501 atgtaccttt tcatataaaa gccctccatc tttttattat attattagtt aaaacaacaa
     4561 agcactcaag tttttcttca gcttataata tatattcgga acaaaaataa taattttaaa
     4621 ggtttttgtc aactttatat aaatttacta tcaaaattca atttattgat tgaaattgat
     4681 aaatttttac caaagtatcc gcaagcacat tgcatttttc aacctattat gatatatttt
     4741 tatttgattt gtattttaaa ttttcaggat ttatactata aatcctgttt ttaatacata
     4801 atagtattta aacagttggc agataattct gctattttgt aaagtatatc taatatttta
     4861 catttatgca tttatatatt tattttattt aaggaggttt ttaaatgact caattaaaag
     4921 gtgcttgtat atttggtcaa tcaggcggac ctacatcagt aataaacgcc agcgcttcag
     4981 gtgtatttca ggaggcttta aagcaggact gcattaccgc agtatatgga gccgctcatg
     5041 gtattaaagg aatattggat gaagtttttt atgatatgag caaagaagat caatatgaat
     5101 tggaattact taaaacaacc ccttcatctg ctcttggttc tgtgcgctat aggctcaaaa
     5161 aagcagaaga cgatgaaaca gattataaga gaattcttga ggtctttaaa aagtacaata
     5221 taagatactt tttctacaat ggcggaaatg attccatgga cacatgcaat aagatcagca
     5281 aatacatgca aaaagcaggt tatgaatgca aagtaatggg tgttcccaaa accattgaca
     5341 atgatttgtg gggtacggat cactgcccgg gttatggaag tgcagctaaa tacattgcta
     5401 catcaacaat ggaaatttat catgatgcaa gagtgtatga taaaggtcag attactgttt
     5461 tggagataat gggcaggaat gcaggctggc tgactgctgc ttctgctctg gcagcttaca
     5521 agggacatgg tccggattta atttaccttc ctgagcttcc ctttgatgtt gacagattta
     5581 ttgatgatgt aacaaaaatt tacaaggcaa atggtaatgc aattgttgct gtttctgaag
     5641 gtataaagga caaaaatggc aagtatattt ctgaatatgg ttcagatcta gctcaaagca
     5701 aagatgcttt tggtcatgca caactaggtg gtctggcttc aactcttgca aactttgcta
     5761 aggaaaatac aggtgccaaa gtcaggggta ttgaattcag tcttctccaa agatgtgctg
     5821 ctcaccttgg ttctcttaca gacgtaaatg aggctttcat ggcaggacag gctgccgttc
     5881 aatatgcagt tcagggaatt accgataaga tggtagcttt tgaaagagct gaaggtactg
     5941 aatataaatg cagcattaag ctgattgatc ttacagatgt agccaatact gaaaagaagg
     6001 taccctttga atggataata aataatggta caggaatatc agatgagtat ataaaatatg
     6061 ctctgccgtt aatacagggt gaatccacac ctccagtaga ggatggactg ccaaggtttg
     6121 ctaaattgaa aaaggtattg gcagctaagt aatctgtaag taacctgaaa gatatcacaa
     6181 ggtgtattat acatttttga aaaatatatc ctggtaacta tcattgagat taatataaac
     6241 ccgctcagtt atattctatg agcgggtttt ctagagtaga aaaacaaaca aaatattctt
     6301 gtatgtagtg tatatacgtg ctataatcaa gatattatat aagtatggtt taattaaccg
     6361 gcagacctta tactccttaa caagttggag cgaagagtca agttaattaa atccaaattg
     6421 ttaaggaggt gtacccatgg cagataagac tcttatttgc aaggactgta acagtgagtt
     6481 tgttttcact gaaggtgaac aagctttcta caaggaaaag ggatttcaaa atgaacctca
     6541 aagatgtccc gaatgcagga aagccagaaa acagcagagg agaaataatt ccggttctca
     6601 taaattcggt gataacagga tgtaatttat ccagggaaat agctgcgggg agagtccttc
     6661 cacaagaact ctccttattt aattgggagt ttatacatac acctgaaata caaccttaca
     6721 attatacgac tttacaactt taaatatagg aggtgcttac ttttgaagga tattcctctt
     6781 tatgaagtac gaaaaataaa agacataaaa gatatgataa actcaagtat tgaaatatac
     6841 ggaagtaaac ctgcattttg ggtaaagcaa aaaggtactg ataaatatct tgcaatatct
     6901 tattacgaat tgaaaaagga tcttgatgct cttggaactg ccttaattga tttaggatta
     6961 aaagataaaa gaatagccat tataggtgaa aatagatatg aatgggcagt atcatatctt
     7021 gcaatagtcg ctggtaccag tattgtagtt ccaattgaca gggaactgcc caagagcgaa
     7081 atcgaaagcc tgctcataag agcagaagtt gatgcaatta ttttttcggg taacaactat
     7141 tcacatatca gtgaagtagc aaaaaaaaat aatgcagtaa agtattacat taatatggac
     7201 ggtaaagaaa ctaattatat tgaaactaat aatacaggat ctgataatac aagttctgat
     7261 aatacaggat ccaataattt tcaaactgat aatacaaaaa cccgtaatac tatccttgcc
     7321 tataaagatc tcatcaaaaa gggaaaagag cttttaaata atggagatgc aaaatttata
     7381 aatgcagcta ttgatgcaga atcaatgagt attctcctat ttacttcggc aaccacagat
     7441 aaatccaaag ctgttatgct atcccaaaag aatatttgta taaatcttat ggctatgagt
     7501 tcatatagac cccaaggata tatttttttc aatacttccc ctgcatcata catacgaatg
     7561 cacatgcgga tttttatgtg ctctatatag aggtgcatca atagcctact gtgaaggttt
     7621 aagacatata caaaagaatc ttcaggaatc gaaggctact gtcatgctgg ctgtgccatt
     7681 aatttttgaa gcaatgtata gacgtatctg ggatcaggct aaaaaaagca gcgcagataa
//
LOCUS       DAOSDD010000213         7649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3523_length_7649_cov_4.672768, whole genome shotgun sequence.
ACCESSION   DAOSDD010000213 DAOSDD010000000
VERSION     DAOSDD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_7649_cov_4.672768"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PK733_15395"
     CDS             <1..840
                     /locus_tag="PK733_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11957.1"
                     /translation="REKWGLCGLMESHHYGWWPSFVSEVAKWSYWTPSPTSDEVLRKI
                     AKRDFGEENVDTVIDAWKAWSEGIRNYVSTNEDQYGPFRIGPSYPLVFRGDVQIPTVP
                     YAMFGGNRICNTNYASRDNGRSSLFQYRLPVEIERLEKMKECFLHGVKCLEEIYEALS
                     EDKKKDAFYMINLGKFIVNCVKTTINIKKWNMLKMQLFIETDESKINEYVDEMIEIGK
                     DEIKNAEATIPLVEADSRLGWEPSMEYMTDVYHLNWKIKQVKLTIEGALPGYKAALRF
                     NHD"
     gene            954..1772
                     /locus_tag="PK733_15400"
     CDS             954..1772
                     /locus_tag="PK733_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ11958.1"
                     /translation="MVNVDIGIIVQLKHVLENGVDNILELGLNACQINCWNAELYTPE
                     NAEKLKEIVKDKVKITSLWAGWSPPAVWNFIDGPLTLGLVPREYRAQRINELKKGADF
                     ANMLGITDVTTHMGFIPENPATTEYRETVVAVREVGAYCKRNNLYFNFETGQETPVTL
                     MRTIEDTGLDNLGVNLDPANLLLYGKANPVDAVDIYGDKIRGIHVKDGMYPTNGHSLG
                     KEMPIGEGLVNFPKLIKTLQKYNYNGPYIIEREISGPQQKIDIIKARDFLYSVL"
     gene            2334..4271
                     /locus_tag="PK733_15405"
     CDS             2334..4271
                     /locus_tag="PK733_15405"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ11959.1"
                     /translation="MVCDYEKVNIISLLYDSVADFAVSELKKYLKMMMPESININICK
                     DENCSTCYEKNIKLGLLEQFDIDNSEVENDLFDDVIHIDVDGLCGVIAGSNARSILLG
                     VYRFLHEAGCRWVRPGIDGEYIPEKDMNNVNVKLHEKAAYRHRGLCIEGAVSYENMAE
                     NIEWAPKVGFNSYFIEFITPYTFFDRWYSHKYNKYKKPEHLTVEMVQEFTAQLEKEIK
                     KRGLVYHAVGHGWTCEPFGIPGLGWDMETYELDEETRKYFAEVNGKRDIWHGVPLNTN
                     LCYSNLEARKKVVDYCADYVLKNNQIDVLHFWFADDVNNHCECEECRKAIPSDFYVKL
                     LNELDDEFTRRGIKAKIVFLLYVDLLWTPEKEKIKNPDRFLILFAPITRTYSSPYSTE
                     KFDGQLPQYNRNKLEFPRSVLENIAYLREWQKMFDGDSLVYEYHFMWDHYNDPGYYDM
                     AKVLFEDIKRLKELKLNGLISDQTQRSFLPTGFGMYLMGRALWNNALEFEDIANDYFS
                     NAFGDDGLKCKRYMEELSKLFNPVYIRGEKGRENKEAAVNLAKVKGVVGSFLPVIKKN
                     IKYENNCIAKSWEYLEFHAGLVTRLADAFKARAEGNETVAAKLWEETSDYAQCNEDAV
                     QPVFDLCTFVQSTKSKFKAST"
     gene            4485..5780
                     /locus_tag="PK733_15410"
     CDS             4485..5780
                     /locus_tag="PK733_15410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ11960.1"
                     /translation="MKTKMTFSATGDSFITRRLPSKNVSFEEIKSIIQMAEAKFTNLE
                     VTAHNQEGFPSAQSGGTWAITSPEVLNDIKDYGFNLIAWANNHTLDYSYGGLKATERY
                     LNQYGFVHAGAGKNLASASTPKYLDCPSGRVALIAATSSFHESWIAGEQRRDVIGRPG
                     VNPLRVVTTYNVTSEDFNHLQGIADDVEINAYDNLIRKEGFLSGLDEGVFMFGKYTFK
                     KSNYNGVTTEPVEKDMQRIIKAIFQARRQADYVIVSIHSHQIKGKNKEQPADFLITAA
                     RSCIDAGADAVIGHGPHVLRGIEIYKNRPIFYSLGNFIFQSETVSNLPADFYEKYGLG
                     HEDDVVDALDKRSGNGTKGLAKNPEAWKSVIPVWTMEDSELKEIKLYPIELGYGLPGY
                     KSGWPALSRDNEISESLRKLSRPFNVDIKINDNIGIIKL"
     gene            6024..7022
                     /locus_tag="PK733_15415"
     CDS             6024..7022
                     /locus_tag="PK733_15415"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ11961.1"
                     /translation="MLSDKLNDIRNNMTELKNERKSELNSELKYEIRNKLKNNLNTLN
                     MFRAIRCMSFNVRLDTPADKLNSWPYRKELAASMLQFHHVDIAGLQEVLINQLNDLSK
                     FLPEYGWIGAGRDDGAEKGEFTPIFYLRERLELMGHGTFWLSETPEKPGSRGWDAICR
                     RTVTWGEFKDRLTDKVFYYFNTHFDHFGRVARVQSAYLLLQKIISIACSNPVIVTGDF
                     NCTINSEAYQLLAGKIHNRFGLEPLRDSRYESERGHHGPSITFHDFKATSIFKLAKKL
                     KDLRKAPASIEWEMGIDFIFVKNSVKVIQHGVLSDTWNGRYPSDHMPVVADLILGN"
     gene            7271..7348
                     /locus_tag="PK733_15420"
     tRNA            7271..7348
                     /locus_tag="PK733_15420"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7306..7308,aa:Pro,seq:cgg)
BASE COUNT         2708 a         1114 c         1607 g         2220 t
ORIGIN      
        1 cgggaaaaat gggggctttg cggtttaatg gaatctcatc actatggttg gtggccttca
       61 tttgtaagtg aagtcgcaaa atggtcatat tggacacctt ctcctacaag tgatgaagtc
      121 ctaagaaaga tagcaaagag agattttggt gaggaaaatg ttgatactgt tattgatgcc
      181 tggaaagcat ggagcgaagg aataagaaac tatgtttcta ctaacgaaga ccaatatgga
      241 cctttcagaa taggaccatc ttatcctttg gttttcagag gcgatgtcca aattcctact
      301 gttccttatg caatgtttgg aggcaataga atatgtaata caaactacgc ctcccgtgat
      361 aatggaagga gctctctatt tcaatacaga ttacctgtag aaatagaaag actggaaaaa
      421 atgaaggaat gttttcttca tggagtaaaa tgtcttgagg aaatttatga agctctttcc
      481 gaagacaaaa agaaggatgc tttttacatg ataaaccttg gtaaattcat agttaattgt
      541 gttaagacaa ctataaacat taaaaagtgg aatatgttaa aaatgcagtt atttattgaa
      601 actgacgaaa gtaagataaa tgaatatgta gatgaaatga tcgaaattgg gaaagatgaa
      661 attaaaaatg ccgaagctac aatacctctg gttgaggcag attccagatt gggatgggaa
      721 ccaagtatgg aatatatgac tgatgtgtat cacctaaact ggaaaattaa acaggtaaaa
      781 cttacaatcg aaggggcatt gccgggttat aaggctgctt tgaggtttaa tcatgattaa
      841 ggcacaaatg cacccaatac acagtgtagc agttgactta attagtaatt tatgactaaa
      901 atataataaa gttgcctatc gctctatcat attttagaaa ggatgatgat aaattggtta
      961 atgtagatat tggtattatt gttcaattaa agcatgtact tgagaatggg gtagacaata
     1021 tactggaact tggcttgaat gcctgccaga ttaactgctg gaatgcagag ctgtatactc
     1081 cggaaaacgc agaaaaatta aaagaaatag taaaagataa ggtaaaaata accagccttt
     1141 gggcaggttg gtcaccaccg gcagtatgga attttattga tgggccgctc acattaggcc
     1201 tggtcccacg tgaataccgt gcacagcgta taaatgagct gaaaaaaggt gcagactttg
     1261 ccaatatgtt gggaataact gatgtcacaa ctcacatggg ctttattcct gaaaacccgg
     1321 caacaacaga atatagagag actgtagtag cagtaagaga agtaggtgca tactgtaaaa
     1381 gaaataatct atatttcaac tttgagacag ggcaggaaac accagttaca ttaatgagaa
     1441 ccattgaaga taccgggctt gataacctgg gtgtgaatct tgatcctgcc aaccttctat
     1501 tatatggcaa agcaaatcct gtagacgcag tagatattta cggagataaa ataagaggta
     1561 tccatgtaaa agatggtatg taccctacga atggacatag ccttgggaag gaaatgccaa
     1621 taggagaagg acttgttaac ttcccgaagc taataaagac gcttcagaaa tataattata
     1681 atggacctta cataatagaa agagaaatca gcggaccaca gcaaaaaatt gatataatca
     1741 aggcaagaga ttttttgtat tcagtattat aaagaaagta tagtaatact actatattaa
     1801 aaagtatagt aatacttcta tgctaatttg aaaagctatg tcttccagga taatttttga
     1861 tagcatcggg aagatgtcgc acagggaaat gtctctatag aaggcattgc ggcccttccc
     1921 tcggatgtat tcattacgag tcagcaaaaa ttaccttcca gaaatgcgct ttctattggc
     1981 aacataacaa agtagtagta atataacatt taataaagca gatgtagcag aacatctgct
     2041 ttattaattt ttgcggcaaa tttattggga aataaatact atataattta cgatttgaaa
     2101 gaactaatga agaatttgca aatcgtaagt tacatcaagg taacagaagc aagttttaaa
     2161 tattttaaac ctgttctata tagaaaatga atattgttca attagttgcg taaatagtta
     2221 aagaatataa aaaatagatg aattataata atgtatgaaa gtatatacaa gtaaaatatt
     2281 agttatatgt ttataaaata tttatacaat ttatcggaag gggaatttat caaatggtgt
     2341 gcgattatga aaaagtaaat atcatatctc ttttgtatga tagtgttgcc gattttgcag
     2401 tatcggaatt aaagaaatac ctgaaaatga tgatgcctga atcaataaat attaacatat
     2461 gtaaagatga gaattgctct acttgctacg aaaaaaatat taaactaggc cttttagagc
     2521 agtttgacat tgataactct gaggtagaaa atgacctttt tgatgatgta atacatattg
     2581 atgttgatgg actatgtggt gttattgcag gctctaatgc aagaagcatt ttactgggag
     2641 tttacagatt ccttcatgaa gcaggatgca gatgggtaag gcccgggatt gatggtgagt
     2701 atattcctga gaaggacatg aacaatgtaa atgtcaaact ccatgaaaaa gctgcttatc
     2761 gtcatagagg actttgcata gaaggtgcgg ttagttatga aaacatggcg gaaaatattg
     2821 agtgggcacc aaaagtaggt tttaattcat acttcatcga gtttattaca ccatatacct
     2881 tctttgacag atggtacagc cataaatata acaagtataa aaaaccggaa catctaacag
     2941 tggaaatggt tcaagaattt actgctcagt tggagaagga gattaaaaaa agaggacttg
     3001 tgtatcatgc agttggacat ggatggacat gtgagccttt cggaatacct ggtcttggtt
     3061 gggatatgga aacctatgag ttggatgaag aaacaaggaa atactttgct gaggtaaatg
     3121 gtaaaagaga tatatggcac ggggttcctc ttaataccaa tctttgctat tccaacctgg
     3181 aagctagaaa aaaggttgtt gattattgtg ctgattatgt tttaaaaaac aaccaaattg
     3241 atgtactgca tttctggttt gctgatgatg taaacaacca ttgtgaatgt gaagaatgtc
     3301 gtaaagcaat accttcagat ttttatgtaa aacttttaaa tgaactggat gatgaattca
     3361 caaggagagg tataaaagcc aaaatagtct ttctactata tgttgacttg ctctggacac
     3421 ccgaaaaaga aaaaattaaa aaccctgatc gtttcctgat cctctttgca cctataacaa
     3481 gaacatatag cagtccttat tctactgaga aatttgatgg acagctgcct caatataaca
     3541 gaaataagct ggaattcccc agaagtgttc ttgaaaatat tgcatactta agggaatggc
     3601 aaaaaatgtt tgatggcgac agcctggtct atgaatatca tttcatgtgg gaccattaca
     3661 atgatcccgg atattacgat atggccaagg tgctttttga agatataaaa agactgaaag
     3721 aactcaaact aaatggactt ataagtgatc agacccagag atcattcctg cccacaggat
     3781 ttggtatgta tcttatggga agggctcttt ggaataatgc tttggaattt gaagatattg
     3841 caaatgatta cttcagcaat gctttcgggg atgatggcct gaagtgcaag cgctatatgg
     3901 aggaattatc caaactattt aatcctgttt atattagagg agaaaaagga agagaaaata
     3961 aggaagcggc tgtaaatctg gcaaaagtga aaggggtagt aggtagtttt ttacctgtta
     4021 ttaagaaaaa tattaagtat gaaaacaatt gtattgctaa atcatgggag tacctggagt
     4081 tccatgccgg gctggttaca agattggccg atgcatttaa agcacgtgct gaaggaaatg
     4141 aaactgtggc agcaaaactt tgggaagaaa catctgatta tgcacagtgc aatgaagatg
     4201 cggtacaacc agtatttgac ttgtgtacat ttgtgcagag tactaaatcc aagtttaaag
     4261 ctagtactta atacaataat aacaatggac aaaatattag aatttcatat gatttttaag
     4321 cgtatagaga atattgggag tgtatagaat atagcgaata ccaaatcttc tttaaaatta
     4381 ctataccggg gtttgggagg ggtttttcct ccctgtttta tgtaagatac ttagaatgta
     4441 atttatttca tcagcaatag aagtgaaatg ggggctagat tattatgaaa actaaaatga
     4501 ctttttccgc aacgggtgat agtttcataa cacgacgatt accttcaaaa aatgtctcgt
     4561 ttgaggaaat taaatctatt atacagatgg cagaagctaa atttacaaat cttgaggtaa
     4621 cagcacataa ccaggaaggt tttccatcgg cacaaagcgg aggcacatgg gctataacct
     4681 cacctgaagt cctaaatgac ataaaagatt atggcttcaa tctgatagcc tgggcaaaca
     4741 accacaccct tgactactca tatggaggcc tgaaagctac agaaagatac ttgaatcaat
     4801 atggttttgt gcatgcaggt gctggtaaga atcttgcatc cgccagtact cccaaatatc
     4861 ttgattgccc atcaggacgt gttgcactca tagctgctac atcaagtttt catgaatcat
     4921 ggatagccgg tgaacaacgc agggatgtta ttgggcgtcc tggagttaat ccccttaggg
     4981 ttgtaacaac atataacgta acatcggagg actttaatca tttacaggga attgctgatg
     5041 atgttgaaat taatgcatat gataatttaa ttagaaaaga aggctttttg tctggacttg
     5101 acgaaggtgt gttcatgttt ggaaaatata cctttaagaa aagtaattat aatggagtta
     5161 ctacagaacc cgttgaaaaa gatatgcaga gaataattaa agctattttt caggcaagac
     5221 gtcaggcaga ctatgttatt gtcagcatac actcacacca gataaaaggg aaaaataaag
     5281 agcagcctgc agattttttg ataactgcag caagaagttg tattgatgca ggagcggatg
     5341 cagttattgg acatggtccc catgtgcttc gcggcattga aatatataaa aatcgcccga
     5401 ttttttacag ccttgggaat tttatattcc agagtgaaac agtttcaaac ctgccagcag
     5461 atttctatga aaagtatggt cttggacatg aagatgacgt agtagacgca cttgataaaa
     5521 ggagtggcaa tggtacaaaa ggattagcaa aaaatcctga agcatggaag tcggtcattc
     5581 cggtatggac tatggaggat agtgagttaa aggaaatcaa actatatcct attgaactgg
     5641 gttatgggct gcctggttat aaaagcggat ggccagccct ttcaagagac aatgagattt
     5701 cagagagctt gcgaaaacta agcagaccat ttaatgtgga tattaaaatt aatgataata
     5761 ttggaattat taaattgtaa gagctataat atcgaacaca ttgggtgaaa tatagcaaaa
     5821 caatggaata atgcaataat atggtttaac tttatgctac tgtaacaagt aatttaccag
     5881 cagcattttc attgagaaaa atagtataac ttcatattac cggaagtatt tttatataat
     5941 aagatatata atatactata ctacggaagg catattataa taaaattaaa tatctataat
     6001 ataaaaaagg tgataaataa ataatgttat cagataaact taacgatata agaaataaca
     6061 tgactgaact aaaaaatgag cgaaagagcg aacttaatag tgaattaaaa tatgaaataa
     6121 ggaataaatt aaagaacaac ttaaatactc ttaatatgtt tagagcaata agatgcatgt
     6181 ctttcaatgt aaggctggat acgcctgcag ataaacttaa ttcctggcca tacaggaaag
     6241 aattggcggc aagcatgctt caattccatc atgttgatat agcaggactt caggaggttc
     6301 tgataaacca gcttaatgac ctttccaaat ttcttcctga atacggatgg atcggcgcgg
     6361 gaagagatga tggagcagaa aaaggagagt ttactcccat attctatctg agggaaagac
     6421 ttgaacttat gggacatggt acattctggc tatctgagac tcctgaaaaa ccagggagca
     6481 gaggatggga tgccatatgc agacgtacag taacctgggg agagtttaag gacagattaa
     6541 cagataaagt attttattat tttaatactc attttgatca tttcggccgt gtcgcaagag
     6601 tgcaaagcgc ctatctgctg ttacagaaga taataagtat agcttgtagc aatcccgtta
     6661 ttgttactgg agactttaac tgtacaataa atagtgaagc atatcagcta ttggcgggaa
     6721 aaatacataa cagatttggc cttgaaccac taagggacag tcgttatgaa tctgaaaggg
     6781 gacaccatgg tccgtccata acttttcatg atttcaaagc tacttctata tttaaattag
     6841 caaaaaagct taaggatctt agaaaagccc cggcaagcat agagtgggaa atggggatag
     6901 attttatatt tgttaaaaac tcagtaaagg ttatacagca tggcgtttta tctgatacat
     6961 ggaatggcag atatccttct gatcatatgc cggtagtggc ggatttgatt ttagggaatt
     7021 gatttgtatg cagagaacac ggtttatgat caagttaaaa atcaaaaggc agattccaat
     7081 atatgaaacc tgcctttttt taatggtcgg agtgacaaga tttgaacttg cgacctcttg
     7141 gtcccgaacc aatcgcgata ccgatagtat acaataaaca accatataat atgattatta
     7201 atgtaattaa aaaagtaaaa tttagtgttg catgttaatt aaaaaagtgt tattataata
     7261 tttagtgtta cggggtgtgg ctcagtttgg ctagagcgct tggttcggga ccaagaggtc
     7321 gggtgttcaa atcacctcac tccgaccaaa aaataaatac cgccatagtt ggcggtattt
     7381 ttgtgtctaa aaataaagat agatttaaca tatcttatac aagcaagata ttttaaatat
     7441 gtttttattt gcgtgacgaa atagcagata ataaaataaa tgtcacataa tgtcgataag
     7501 ttttgtaaga ttttagaagg atattacttt ttctgtgttg aagtagtaaa tataaaagta
     7561 tatatgaagg tgtgtatctt atatggatgt aattaaaaat gtaatttggt tttaaataat
     7621 atcttaatta gcaatagcgg aattggtta
//
LOCUS       DAOSDD010000214         7639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3528_length_7639_cov_5.526371, whole genome shotgun sequence.
ACCESSION   DAOSDD010000214 DAOSDD010000000
VERSION     DAOSDD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_7639_cov_5.526371"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="pdaA"
                     /locus_tag="PK733_15425"
     CDS             complement(<1..1178)
                     /gene="pdaA"
                     /locus_tag="PK733_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HOJ11962.1"
                     /translation="MNNENKLKANSNNNKRNGSNKNNEKNGKGKKGANKKLARKKLKL
                     VLLITAVLFFLFGFGISYLYSTYKAKNNADNINNSNSSKGTTSEGANEKGGTDKESKP
                     DDSGKKNGQQGTEAGKDTDVPDNKNTADKNNGSDGNQSDTKLPGAGGPAPSNPGAPNV
                     EKLNVEALSKLDNTKYSWWIKLNKEHKTPEITDTIKKMIDKHDGIYIGDTSQKVIYLT
                     FDEGYENGYTPKILDVLKDNDVKAIFFVTGPYIQKNPELVKRMLDEGHQVGNHTINHP
                     SLPDMSYEKLENELLGLDKQFYESFNSSFKYMRPPMGEYSERTLEASKQLGYKTVFWS
                     FAYDDWYTDKIRGADYAYNKVMENIHNGAVLLLHAVSKDNTEALDRIIKDIIAQGYEI
                     "
     gene            complement(1446..2327)
                     /locus_tag="PK733_15430"
     CDS             complement(1446..2327)
                     /locus_tag="PK733_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ11963.1"
                     /translation="MRNSKTSIKFHPILLLAVFLFFAAGHGIEFICAFVSISIHEAAH
                     IFAASLMGLKIYNVNIHLAGLNAQIEENYCGTWQKIIMYICGPITNVLLFGAVAVIKA
                     YFEKLKYESPDLVITFLRVNIYLAVFNIIPILPLDGGRILCEILTRNKGLFAAHKYMR
                     IISFIILGFFFIIGVIQAFYYYNFNILLIGLYILFIMKSINQEAAFMNIKQLLYRRSR
                     ILKKGVYPARSLVVMDNVPMGEVVKNMDFDGFHIVYVLDDKFKLINTLTEQDIIDGLI
                     ENDGEITIGEFMVNKVG"
     gene            complement(2332..3174)
                     /locus_tag="PK733_15435"
     CDS             complement(2332..3174)
                     /locus_tag="PK733_15435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ11964.1"
                     /translation="MENVAVKPRYPRNNAYYRRRRAYTRDKSKLSETIAKQLVISIMI
                     FLIIWLIKGINTSVTNFISSKVGWTLSYDTDLKDVPNRISNYFDRIINGHLWEGDLYN
                     KSNENAETGEKEPVNQNEAQNYESDIKDASEESNQTDGSTDLMNIELITPVKGFVSSY
                     FGERIDPITQKSRFHYGLDIEADKGAEIKAAADGEVLEISEDRMYGRNIKIKHPGGII
                     TVYAHCSKITRVDGEKVRQGDIIAEVGDTGYSTGTHLHFEIWKNGKVLDPLSFIDVPL
                     DMTE"
     gene            complement(3408..3806)
                     /gene="mgsA"
                     /locus_tag="PK733_15440"
     CDS             complement(3408..3806)
                     /gene="mgsA"
                     /locus_tag="PK733_15440"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOJ11965.1"
                     /translation="MNIALIAHDKKKELMIDFCIAYKSILQDHTLFATGTTGTLIIEA
                     TGLNVNKFFPGPMGGAQQIGARIACNEMDVVIFFRDPLTAQDYEPDVNSLVRLCDIHN
                     IPVATNIAAAEVLIKGVERGDLEWRNLISK"
     gene            complement(3839..4123)
                     /gene="minE"
                     /locus_tag="PK733_15445"
     CDS             complement(3839..4123)
                     /gene="minE"
                     /locus_tag="PK733_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOJ11966.1"
                     /translation="MLQDILNFFSRPKASKDVAKERLKLVLIHDRANVSPQFLEMIKS
                     EIIKVITNYIEVDERDLDIQLTRTQSEDGESVVPALIANIPIRNVKGKKE"
     gene            complement(4138..4938)
                     /gene="minD"
                     /locus_tag="PK733_15450"
     CDS             complement(4138..4938)
                     /gene="minD"
                     /locus_tag="PK733_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOJ11967.1"
                     /translation="MGEVIVITSGKGGVGKTTTTANVGTGLALCNKKVVLIDADIGLR
                     NLDLVLGLENRIVYDLVDVVEGRCKLRNALVKDKRYEGLFLLPAAQTRDKSSVKPDQM
                     LKLCDELRKEFDFAIIDCPAGIEQGFNNAVVGADRAVVVTTPEVSAVRDADRVIGLLE
                     SKELRDPKLLINRIRTDMVKRGEMMSIEDVKDILAVSLIGIVPDDEKIVVSTNRGEPA
                     VTDPKSLAGQAYRNIARRILGEEVPLLDLETDEGFMLKLKKFLGFKTT"
     gene            complement(5054..5734)
                     /gene="minC"
                     /locus_tag="PK733_15455"
     CDS             complement(5054..5734)
                     /gene="minC"
                     /locus_tag="PK733_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOJ11968.1"
                     /translation="MNDSEIVFKATNNGLILIMNEELDFDSIFNQIDKKLTAAGKFFK
                     GASLVVKYRGRKLLSDEIDRVVRLMEEKTEAEVKSFQEDTEFETAIQDRDQEIEKPIN
                     KISDLYFDGTDEGITKFYRGTVRSGQLISFNGNIVILGDVNPGAEVLASGNIIVMGWL
                     RGTVHAGMYGNSKAIVASLNLQPVQLRISNIITRPPDDINSRGPAIPEIAYVKDDMIY
                     IERFLPQR"
     gene            complement(5867..6412)
                     /gene="mreD"
                     /locus_tag="PK733_15460"
     CDS             complement(5867..6412)
                     /gene="mreD"
                     /locus_tag="PK733_15460"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOJ11969.1"
                     /translation="MRFKGTVVKILVYALSIIIIILFQTTISDYMKINNVKPNLILVY
                     TVCTSILEGSKGGGIIGLFAGLALDIIAGKIIGFYALFGMYLGVIAGMSNRRLYKDNI
                     IVVIFFTFFLTFVYESGVYLLSNPEITRSIMLYALKNIIFTEALYNGGLSIIVHILVM
                     KINARFNFGDDYRQNTSAKRR"
     gene            complement(6409..7284)
                     /gene="mreC"
                     /locus_tag="PK733_15465"
     CDS             complement(6409..7284)
                     /gene="mreC"
                     /locus_tag="PK733_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ11970.1"
                     /translation="MRRLLSLIKNKYFSLIFASVILIIVIMVSTLNTDSFTNISNILS
                     VPLSPLQKLLSSSGQKVDSFFTTFKEIKTLKEENETLKIRVNELEKENRELNSYRDKI
                     VELRLALNLKAQFDDYEIIGANVIAKEPGNWFNVFKIDIGSKDGVDVDYPVISSTKGL
                     VGRIISADSTSSKVVSIIDEDSVISGWILKSGGGHVMLKGDLTLKEQGLCRMYYIPID
                     VDVAVDDVVETSGLGGIYPKGILVGKVREVRKVTNELDRYAIIDPEVDLKRLEEVFVL
                     KRKNDNEKTGNSRGK"
     gene            complement(7300..>7639)
                     /locus_tag="PK733_15470"
     CDS             complement(7300..>7639)
                     /locus_tag="PK733_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ11971.1"
                     /translation="RDMVSGLPKNITITSSEITEALKDPISAIVDAIKYTLERTPPEL
                     ASDIMDRGITLTGGGALLSGLDKLIGEETCMPVTIAENPLDCVALGAGKVLEGIETLR
                     KVLVSPRKLV"
BASE COUNT         2219 a         1656 c         1056 g         2708 t
ORIGIN      
        1 cttatctcat aaccctgagc tatgatatcc tttattattc tatccagtgc ttctgtatta
       61 tcctttgata ctgcatgtag gagcagaact gcaccattgt gaatattttc cattaccttg
      121 ttgtaagcat aatccgcccc tctgatttta tctgtatacc agtcatcata agcaaaactc
      181 caaaacacag ttttataacc aagttgtttg gacgcctcca atgttctttc actatattct
      241 cccataggtg gacgcatata cttaaagctg gaattgaagc tttcataaaa ctgcttgtcc
      301 agtcccagca gctcgttttc aagtttttcg taactcatat cgggcaaact tggatgattt
      361 attgtatggt ttccaacctg atgaccttca tccagcattc tctttaccaa ttccgggttt
      421 ttctgaatat aagggcctgt tacaaaaaag atggctttaa catcattatc cttaagaaca
      481 tcaagtattt taggagtata tccattttca tatccttcgt caaaagtaag gtaaataacc
      541 ttctgtgaag tatctccaat atatatacca tcatgcttgt caatcatttt cttaatagta
      601 tcagttatct cgggagtttt gtgctccttg ttaagcttta tccaccagga atatttagta
      661 ttatccagtt tgctgagtgc ttcaacattc aatttctcaa catttggtgc tccagggttt
      721 gaaggtgccg gtccgcctgc accgggaagt ttagtatcag actggtttcc atcgctgcca
      781 ttatttttat ccgcagtgtt tttattatca ggaacatctg tatcttttcc agcttcagtc
      841 ccctgttgac catttttttt cccactatca tcaggtttgc tttctttatc ggttccgccc
      901 ttttcatttg ctccttctga ggttgttccc ttactggagt tagaattatt tatgttgtca
      961 gcattgtttt ttgctttata agtgctatat aaatatgaaa tgccaaatcc aaacaagaaa
     1021 aataatacag ctgttatcaa taaaaccagc ttcagttttt tgcgagctag ttttttattt
     1081 gctccttttt taccttttcc atttttctca ttattcttat tgctgccatt tcgtttattg
     1141 ttattactgt ttgcctttag tttattttca ttattcatag ttttcatcct ttgcttttaa
     1201 aatacgagac ttacgcttcc ggttcaaata caccagctga acataattaa ttatatcagt
     1261 cattcatatt gaagacaagc tatctaatta actggggtgc attttcttcc tttgaacaaa
     1321 gtttacggat tatttattat gcttaaggca atggaaaatt cgtaattcac gacacagatg
     1381 tcgtgaaaaa agaaggactg tccccctgat ttctccccct gatttcttgt ttttcactct
     1441 gttgtctagc ccaccttgtt taccataaac tccccgatgg ttatttcacc gtcattttca
     1501 atgagcccat caataatatc ctgttccgtc agtgtgttaa tcagcttgaa cttgtcgtcg
     1561 agcacatata ctatatgaaa gccatcaaaa tccatgtttt ttacaacttc acccatagga
     1621 acattatcca taactaccag gcttctggca gggtaaacac ctttttttag tatccttgaa
     1681 cggcggtaca ataattgttt tatattcatg aaagctgcct cctgatttat tgatttcatt
     1741 ataaacaata tgtataaccc aattaataga atattaaaat tatagtaata aaatgcctga
     1801 atgactccaa ttatgaaaaa aaatccaagt ataatgaatg atattatcct catatatttg
     1861 tgtgcagcaa ataagccttt atttcttgtt aatatttcac ataatattct gccgccatca
     1921 agtggtaaaa tgggtattat attaaataca gccaaatata tgttcacccg taaaaaagtt
     1981 atcaccaggt ccgggctttc atattttaac ttttcaaaat atgctttaat cactgcaact
     2041 gccccaaaca gcaatacatt agtaataggg ccgcatatat acataattat tttctgccat
     2101 gttccgcagt aattctcctc aatctgggca ttcaaccctg caagatgaat atttacattg
     2161 tatattttca gtcccattaa cgatgccgca aatatatgag cagcttcatg tatggatatg
     2221 gatacaaaag cacaaatgaa ctctattcca tgaccagcag caaaaaataa aaaaactgct
     2281 aacagcaata tgggatggaa ttttattgag gtttttgaat tgcgcatcaa gctactccgt
     2341 catatcaaga ggcacatcaa taaaagagag gggatctaat actttcccat tcttccatat
     2401 ttcaaaatgc aaatgtgtac ctgtagaata tccggtgtct ccaacctctg caattatatc
     2461 tccctgtcta accttctctc catctacccg ggtaattttt gaacaatgag catatactgt
     2521 tatgatccct cccgggtgct ttatctttat atttcttccg tacattctgt cttcggaaat
     2581 ttccaatacc tctccatcag ccgcagcttt tatttcggca cctttatctg cttcaatatc
     2641 cagaccataa tggaatcggc ttttttgggt aataggatcg atacgttcac caaaatatga
     2701 tgacacaaat ccttttacag gagtaattag ttcaatattc atcaggtctg ttgatccatc
     2761 agtctggtta gattcctctg aagcatcttt aatatcagat tcatagttct gggcttcatt
     2821 ttgattaaca ggttcttttt ccccggtttc cgcattttca ttacttttat tatagagatc
     2881 cccttcccat agatgcccgt ttattatcct gtcaaagtag ttgcttattc tatttggaac
     2941 atcttttaaa tctgtgtcat atgataaagt ccatcctact ttactactga taaaatttgt
     3001 tactgatgtg ttaattcctt ttattagcca gatgattaaa aaaatcataa tagatattac
     3061 taattgtttt gctatagttt ccgatagttt gcttttatcc ctggtataag cccttcttct
     3121 tctgtaatag gcattattgc ggggatatct tggttttaca gctacatttt ccatgcaccc
     3181 acacccttcc ttaatcagag attccatcat ataatttata ttgtacaagt gtgagtttta
     3241 ttacaataaa gaatccggta tgaaaagcaa agtacatacc ggacatgttt tcgttttatt
     3301 tatatcttta aatctttaag ttgcttaaaa atttttcttt tcacttataa tgtttatact
     3361 gtttataatg cttatttaaa tcttttctca taaatcgcca tctgctatta tttgctaatt
     3421 aaattcctcc actccagatc tcctctttca actcccttga ttaacacctc agctgcagca
     3481 atattagttg caacaggtat attgtgaata tcacagagtc tgaccaaaga atttacatca
     3541 ggttcgtaat cctgagcagt aagagggtca cgaaaaaata taacaacatc catttcattg
     3601 cacgcaatcc ttgcccctat ttgctgagcc cctcccatag ggccggggaa aaatttattt
     3661 acatttaaac ctgtagcttc aataattaat gttcctgtag tacctgtagc aaataaagta
     3721 tgatcctgaa gtattgattt atatgctata cagaaatcaa tcattaattc ctttttttta
     3781 tcgtgagcta tcagtgctat gttcatattt attctgcaaa cctactcttc ctgtaatcct
     3841 actctttttt acccttcaca tttcttatag gtatatttgc tataagtgca ggtacaacac
     3901 tttccccatc ttcgctttgt gtccgtgtca gttgaatgtc aaggtctctt tcatcaacct
     3961 ctatataatt ggttattact tttattattt cactctttat catctcaaga aactgaggag
     4021 aaacatttgc cctgtcatgg attaaaacca gttttaatct ctcctttgca acatccttag
     4081 atgctttagg tcttgaaaaa aaatttaata tatcctgcaa catatttaat ctccttttca
     4141 ggttgtttta aaccctaaaa atttctttag tttcaacata aatccttcat ctgtttcaag
     4201 atccaataat ggaacttcct cacccaatat tctccttgct atattacggt aagcttgtcc
     4261 agccaatgat tttggatcgg ttactgctgg ttctcctctg ttggttgaaa ctacaatttt
     4321 ctcatcatca ggtactattc ctatcaaact aacagccagt atatccttaa catcttcaat
     4381 gctcatcatt tcaccgcgtt ttaccatatc agttcttatt ctatttatca aaagctttgg
     4441 atctcttaac tcttttgact ccagaagtcc aattacccta tcggcatccc ttaccgcaga
     4501 tacttcaggt gttgtaacaa ccacagccct gtcagcccct acaacagcat tattaaatcc
     4561 ttgctcaata cctgcagggc agtcaattat tgcaaaatca aactcctttc ggagttcatc
     4621 gcagagtttt aacatctgat caggtttaac cgaagattta tcccttgttt gagcagcagg
     4681 caaaaggaac aggccctcat aacgcttgtc ctttacaaga gcatttctta acttgcatct
     4741 tccttcaact acatccacca aatcataaac aatcctgttt tccagcccca gaaccagatc
     4801 caaattcctt aaaccgatat cagcatcaat caaaacaact tttttattac aaagggcaag
     4861 gccggttcct acattagcag tagtggtagt tttacctact ccaccttttc ctgatgtaat
     4921 tacaatgact tctcccatta gcaaataacc ccccatatat gtatgtatat atatgcacct
     4981 aaaaaactct atatctattt tactaatttg tcaatcaaat agtcaatcaa ataatcaatc
     5041 ctttaattct attttacctt tgtggcaaaa atctttcaat ataaatcata tcatctttaa
     5101 catatgcaat ttctggaatt gcaggtcccc tggaatttat atcatcagga ggacgagtaa
     5161 ttatattgga aattctcaac tgcacaggtt gtaaattaag tgaagcaact atagccttgg
     5221 aattaccata catccctgca tgaacagttc ccctaagcca acccataact attatattgc
     5281 ctgaagccag gacttcagca ccagggttaa catctccaag gataacaata ttaccattaa
     5341 aactgattaa ttgtcctgag cggactgttc ctctgtaaaa tttggttata ccttcatctg
     5401 tcccatcaaa atatagatca ctgattttat taataggttt ttctatttcc tggtctctat
     5461 cctgaatagc tgtttcaaat tccgtgtctt cctggaagct ttttacctct gcttccgtct
     5521 tttcttccat cagtcttaca actctgtcga tttcatcaga caataatttg cgtccacggt
     5581 atttaacaac cagagaagct cctttaaaaa atttaccggc agctgtcaac tttttatcaa
     5641 tttgattaaa tatagaatca aaatccagtt cttcattcat tattagaatc aacccattat
     5701 ttgtcgcttt aaacactatc tctgagtcat tcatgccaat actaccccca aaagttattt
     5761 agcatttttc atacaacact atactgcact attctatatt atactacatt tgtctttgcc
     5821 ttatattaat tatataatat taatatacta catgttttat catggtttac cgtcttttcg
     5881 cactagtatt ctgccggtaa tcatccccga aattaaacct tgcattaatc ttcatgacca
     5941 aaatatgcac tataattgat aggcctccat tatataaagc ttcggtaaat attatgtttt
     6001 tcagtgcata aagcatgatg cttcgtgtta tttccgggtt gctcaacaga tatactccac
     6061 tttcataaac aaatgttaga aagaatgtaa aaaatattac aacaataata ttatctttgt
     6121 acaaccttct atttgacata ccggcaataa ctcccaggta catcccaaat aaggcataaa
     6181 agccaatgat tttgcccgca attatatcca aggccagtcc tgcaaataat ccaataatcc
     6241 ctccgccctt actgccttca agtattgaag tgcatacagt gtataccaga ataagatttg
     6301 gttttacatt gttaattttc atataatctg aaatagtagt ttgaaaaagt ataattatta
     6361 ttatacttaa agcataaact aatattttga ctactgtccc cttaaatctc attttcccct
     6421 cgagttacct gttttctcat tatcattttt cctcttcaac acaaatactt cttcaagccg
     6481 ctttaaatct acttccggat cgataatggc atatctgtcc aattcattgg taacctttct
     6541 gacttctcta acttttccca caagaatgcc tttagggtat attcctccaa ggcccgatgt
     6601 ttcaaccaca tcatccacgg caacatccac atcaattgga atatagtaca tacggcataa
     6661 gccctgctct tttaaagtca gatcaccttt taacattaca tggccccctc cgctcttcaa
     6721 aatccagccg cttatgacac tatcttcatc aatgatcgac actactttgg aggatgtgct
     6781 gtcagcactt ataattctac ccactaaacc ttttgtactg gatatgaccg gatagtctac
     6841 atccactcca tctttactgc caatatcaat tttaaataca ttaaaccagt ttcccggttc
     6901 cttagctatt acatttgcgc caataatttc atagtcatca aattgagcct tgaggtttaa
     6961 ggctaacctc agttctacaa ttttgtccct gtagctgttc aactctctat tttctttctc
     7021 aagttcattt actcttattt tcagagtttc attttcttcc tttagagttt ttatttcctt
     7081 aaatgtagta aaaaaagagt ctaccttttg ccccgaacta gatagtaatt tctgaagggg
     7141 tgaaagcgga acacttaaaa tatttgaaat atttgtaaag ctatctgtat ttaaagttga
     7201 taccattatc acaattatta gtataactga agcaaatatg agactgaaat atttattttt
     7261 aattaagctt aataatcgcc tcaagggttt aactcccttc tacacaagtt ttctgggaga
     7321 aactaaaact ttcctcaatg tttcaatacc ttccaggact ttacctgcac caagagctac
     7381 acaatccaga ggattttctg caatagttac aggcatacag gtttcctcgc ctataagctt
     7441 atcaagccca ctcagtaatg cccctccacc tgtaagtgta atacctctgt ccataatatc
     7501 agatgccagt tcaggtggag tcctttccaa tgtatattta atagcatcaa caattgcact
     7561 gatagggtcc tttaacgctt cagttatttc agaagaagtg atagtgatgt ttttaggaag
     7621 tccggatacc atatctctt
//
LOCUS       DAOSDD010000215         7620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3544_length_7620_cov_5.177925, whole genome shotgun sequence.
ACCESSION   DAOSDD010000215 DAOSDD010000000
VERSION     DAOSDD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3544_length_7620_cov_5.177925"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..307)
                     /locus_tag="PK733_15475"
     CDS             complement(143..307)
                     /locus_tag="PK733_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HOJ11972.1"
                     /translation="MRIREGVIPTALGIAVTVTGIALKEVNPKISWGVTGFGLAHILL
                     GGIDLVQHSR"
     gene            complement(465..1241)
                     /locus_tag="PK733_15480"
     CDS             complement(465..1241)
                     /locus_tag="PK733_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOJ11973.1"
                     /translation="MHSIDICYLYPELTNSYGDRGNIFTLQKRCEWRNIDVNIHNISL
                     KDNFDPNRFDIVFFGSSQGYEKQIILGDFKAVKANAVKEAVENNVVFLCIGGGYQLMG
                     KYLETTDGTKLECPGILDIYTIDSNKRMTGHLIFKCNFPASGSKGSSVGNKEPDDISS
                     DISVVSFENHSGKTYLGSGVSPLGKVLCGCGNNGEDGYEGAVYKNVYCSYGHGSLLPR
                     NPLLADYLITLAMKRKYIDFDTLQPLDDWLENQAHLSVLK"
     gene            complement(1280..2698)
                     /locus_tag="PK733_15485"
     CDS             complement(1280..2698)
                     /locus_tag="PK733_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HOJ11974.1"
                     /translation="MNKIRLILAIFVSRLTMIICRLTNRGGTALPGKIALKIYPGILG
                     EITRSFKIITVTGTNGKTTTTGIIAKILESAGIKYISNKSGANLLSGITGTFVSAVNF
                     LGQSTCSTALIEIDEAVLSKISFHFKPDIIVVTNFFRDQLDRYGEIYTVLEGIKNAIN
                     NLPESLLVLNADDSLCASLGRNVVNKVIYYGINNPGIEIADEHKDAIRSEGNSDAQFC
                     IYCKNKYEYAWRTYGHLGEFKCMSCGYERPEVQVACTGIDKMTDSYSSIRIHFDGYDT
                     ENSNNINISLPGLFNVYNALAASACGIAANIPYEVIIPALSEYKSGFGRMEEINAGGK
                     TIKIILVKNPAGFNQVLDYLSMEDKNIVIGFIVNNMAGDGVDTSWLWDVDFEKLNPME
                     SKIQKIFISGARGFDMGVRLKYAGLPVENLHMTEDVAALLDEGLEAVDENTRFFIMPT
                     YTAMLEIRKILKKRYNLKNYWK"
     gene            3084..3683
                     /locus_tag="PK733_15490"
     CDS             3084..3683
                     /locus_tag="PK733_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ11975.1"
                     /translation="MENNEKVLIEKSQNGDIEAFEKLIEAYQRKVFNIALGMFGNYED
                     ASDMAQEVFIRIFKSIKSFKGQSLFSTWLYRITTNVCLDELRKRKNKNVISIDEEIHL
                     DDGEGVVRQIIDESPSPDVIAEKKELKKVVNDAILELSAEHKEVIILRDIQGFSYDEI
                     AEIIKCPQGTVKSRINRARNILKEILKNKKELWDKEYVK"
     gene            3704..5221
                     /locus_tag="PK733_15495"
     CDS             3704..5221
                     /locus_tag="PK733_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ11976.1"
                     /translation="MLNCSQIKNLLASYIDNEMDERARIEFEEHISICSSCREEMQDI
                     IKVVNMLRSLPEAELPEDFSEKLHQKLVRVKEEELHGRKWLPAKLFTVKNSYLKALST
                     VAAGALVIFALKGIFFNGFLNRNKIAKLENSTKMSKSVPEELMAKDSILNESAEGESI
                     ETRSSSNDFTVNDSTANDSVAIGSAANELVENKSTAMIKGDFKGAGSSEKPIDQQDIG
                     IMFTEGTARSKDSSELLKYSDKEAKEKNSIEGSETGVLDDKNATDKSNIKSFTADDFQ
                     SANGKSEADKGSETDMVPETNTGSESDIKLSSVRGIEDSNVTIMMSTESACMKNLQEH
                     ELLFGNNKIKVSVLIEGNTAEQEKVRSVIMESGLLSDEEKGNMIFKEYNEKSEESARE
                     GESTFALVELNMRGINLNPLIDAIIYSKNELNAQVDCEEPIWDDGIKLKYDELNTKLL
                     EINKQISATQEKSDSSSQSDTEKLIADRNEILNNIEELLQNTEYIAEIRINLTRK"
     gene            5399..>7620
                     /gene="polA"
                     /locus_tag="PK733_15500"
     CDS             5399..>7620
                     /gene="polA"
                     /locus_tag="PK733_15500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOJ11977.1"
                     /translation="MVYKKMDNKKLMLIDGNSILNRAFYGLKGSQLLATSYGLYTNAV
                     YGFLNILFKYQDEEQPQYICVAFDMKAPTFRHDQYEGYKANRKGMPDELAVQMPVLKD
                     VLDAMNITRVEYSGYEADDLIGWLSRCGEDKGMDVVIVTGDRDSLQLISEKTRIKLPS
                     TRFGKTNTEEYNLSSFYEKYSISPTQFIDVKGLMGDPSDNIPGVRGIGEKTALELIRN
                     FGSIEEIYNNINKVDKKGVREKLETGRDMAFLSRQLATIERKVPEELCQIDNLKIRGY
                     DTERLYQLFKKLEFKSLIEKLGLHSVDSKNKDTGITYMEKAGENSNDENSQGENSQVS
                     NNDQQNNLKRIIYIKNAGDLAELRSRLLSCNEIGISHILEKTPEKGELVGELAAFALS
                     WKESSRIYSAYVELTEDINEENFIDEFKDIFLDESVKKIGHNIKNLLLYLKCRDISLK
                     GLAFDTMIAAYILNPSRNTYMVSELTADYLKKEIEHLEELTGKGKNQICYREVPCNHL
                     ARAATGYSEAILELKQVLEKEIRSNQQEMLYYQIELPLVEVLASMEYYGFKINKDELS
                     TFSVELDEKIGTLTSEIYSLAGEEFNINSTKQLGAILFDKLGLPVIKKTKTGYSTDAE
                     VLEQLASMHSIVSSILEYRQLMKLKSTYVDGLLSVINERTDKIHTSFNQTVVVTGRIS
                     STEPNLQNIPYKLEMGRKIRKVFVPSSKEYILTDADYAQIELRVLAHISGDKSRISAF
                     "
BASE COUNT         2624 a         1228 c         1475 g         2293 t
ORIGIN      
        1 tacttgagag aagagtttcc cctgcccagc tacagtacct gcactaatgc ttcttccaat
       61 aatttcttca aaaataaaat atgcaccccc gaattagggg tggcttgccc tgatccggca
      121 tttatgcgta gtgaatgtaa tttcagcggg aatgctgtac caggtcaatt cctccaagta
      181 atatatgtgc cagcccaaaa cctgtaactc cccatgaaat tttagggtta acttctttta
      241 atgcaatacc tgttacagtt actgcaattc ccagtgctgt aggtatgaca ccttccctga
      301 ttctcatatt gaatagcctc ctctgccctt atttagttct ttgatttagt tttttgttaa
      361 gccaggactc tgttattttg tattattttg tggagttttt cttatttaat acatttaata
      421 tagtcaaata ttattaataa aaattgatcg agcagaggta caatttattt taatactgat
      481 agatgggctt gattttccaa ccagtcatcc agtggctgaa gcgtatcaaa atcgatatat
      541 tttcgcttca tggcaagggt tattaaataa tctgccagca gcggatttct tgggagaagg
      601 cttccatgcc cgtaagaaca gtatacattt ttataaacag ctccttcata gccgtcttca
      661 ccattattgc cacagccgca aagcactttc cccagaggtg aaacaccaga cccaaggtag
      721 gtcttgccag aatggttttc aaagcttacc acagatatat cgctggatat gtcatcaggc
      781 tctttatttc caaccgatga tcccttagat ccagaagcag gaaaattgca cttaaatata
      841 agatggcctg tcatcctttt attggaatca atagtgtata tgtccaaaat acctgggcac
      901 tccagctttg ttccatctgt ggtttccagg tatttcccca taagctggta tcctccccca
      961 atacatagga agacaacatt gttttcaaca gcctctttta ctgcatttgc tttgacagct
     1021 ttgaaatctc ccagaattat ttgcttttca tagccttgac tgctgccgaa aaaaactatg
     1081 tcgaaacggt tgggatcaaa attatcttta aggctgatat tatgaatatt tacatctata
     1141 ttccgccatt cacatcgttt ttgaagggta aatatatttc ctctgtctcc atatgaattg
     1201 gtaagttcgg ggtagaggta acaaatatct atgctatgca ttgttatata acaaactccc
     1261 ttctatttat atatctaaat tacttccaat aattctttaa attgtatctt ttcttcaaaa
     1321 ttttccttat ttccagcatg gctgtgtatg taggcataat aaaaaacctg gtattttcat
     1381 caacagcctc aagtccttca tccaaaagag cagcaacatc ttctgtcata tgcaggtttt
     1441 caacgggcaa tcctgcatat ttgagccgta cacccatatc gaagcctctt gcacctgata
     1501 taaatatttt ttggatttta ctttccatag ggttcagttt ttcaaaatct acatcccaga
     1561 gccatgatgt atcaacacca tcaccagcca tgttgttaac aataaaccca atgacaatat
     1621 tcttatcttc catggataga taatcaagca cttgattaaa acctgccgga tttttaacaa
     1681 gtatgatttt tattgtttta ccccctgcat taatctcttc cattctgccg aaacccgatt
     1741 tatattcact taaagcaggg attataactt cgtaagggat atttgccgca attccacatg
     1801 cagaagctgc aagagcgtta tacacgttaa ataagcccgg taaactgata tttatattat
     1861 tgctattttc tgtatcatag ccatcaaaat gaatcctgat gctggaatag ctgtctgtca
     1921 ttttgtcaat acctgtacaa gcaacttgta cttccggtct ttcatatccg caagacatac
     1981 atttaaattc tccaagatga ccgtatgtac gccaggcata ctcatattta tttttacagt
     2041 atatgcaaaa ctgtgcatca gaattaccct cgctcctgat tgcatcttta tgctcatctg
     2101 ctatttcaat gcctggatta tttatgccgt agtatatcac tttgtttact acatttctgc
     2161 caagacttgc acaaagtgaa tcatcagcat ttagaactag cagggattcc ggcaaattat
     2221 ttatagcatt ttttatgcct tcaagcactg tataaatttc tccgtaacga tccagctgat
     2281 ctctgaaaaa attagttacg acgatgatat caggcttaaa atgaaaggat attttgctta
     2341 acacagcttc atcaatctca atcagtgcag ttgaacaggt actctgaccc aaaaaattta
     2401 ccgcactaac aaaagttccg gttataccgc ttaaaagatt tgcgcctgat ttatttgaaa
     2461 tgtattttat accagcggac tccagtattt tagcaattat tcccgtagtt gtggtcttac
     2521 catttgtccc tgtcacagta attattttaa acgatctggt aatttcccca agtataccag
     2581 gatatatttt taaggctatt ttaccaggta aagctgttcc cccgcgattt gtgagcctgc
     2641 aaattatcat agtcagtctg gaaacaaata ttgccaatat taacctgatt ttattcatta
     2701 tttccacaaa atcctccaac accacgtata aatgtatata agtactacta tgccaatttg
     2761 gaaaacgatg tctaccggga taatttttga tagcatcttg caacataaca aagtagtaat
     2821 tgtatttatc ataacataat tatacactat ttttcgcaaa aattatcata agttatatat
     2881 ttagggtaca tttaatttgt ctgaaggaaa tattggtaaa gagcactgta acaatagtcc
     2941 atggtaaata tttctccaga tttgacaaat gcacccatac attttatact tttactttag
     3001 ttgtttaaaa aaatttaaag acaatgtata atgagtaaag ggaatctata cttccccgtt
     3061 gtaaaaataa agggggtaac ccgatggaga acaatgaaaa agttttaata gagaaatccc
     3121 aaaatggtga tattgaagct tttgaaaagc tgatcgaagc ttatcagaga aaagttttca
     3181 atattgctct gggtatgttc ggaaattatg aggatgcaag tgatatggcg caggaagtgt
     3241 ttataagaat ttttaagtct attaaaagct ttaaaggcca atcattattc tcaacatggt
     3301 tatacaggat aacaaccaat gtttgtctgg atgaactaag aaaaagaaaa aataaaaatg
     3361 tcatatctat tgatgaagag attcaccttg atgatggtga aggggtagta cgtcagatta
     3421 ttgatgaaag tccatcaccg gatgtaatag ctgaaaaaaa agaattgaaa aaagttgtaa
     3481 atgatgcaat tttagaatta tctgcagagc ataaagaagt gattatatta agagacatac
     3541 aggggtttag ttacgatgaa atagctgaga taataaaatg tccgcaggga acggttaaat
     3601 caaggataaa tagggcaagg aatatactaa aagaaatact taaaaataaa aaggaacttt
     3661 gggataaaga atacgtcaaa taataaggga aggagggttg gagatgttga attgcagtca
     3721 aataaaaaac ttgctggcat catatataga taatgaaatg gatgaaagag cacggattga
     3781 atttgaagaa catattagca tttgctcttc atgcagggag gaaatgcagg atattataaa
     3841 agttgtgaat atgctgagaa gtcttcctga agctgagctt cctgaagact ttagtgaaaa
     3901 gctgcatcaa aaacttgtca gagtaaaaga ggaagaatta catgggagaa aatggcttcc
     3961 ggcaaaacta tttactgtaa agaatagtta tttaaaagct ttatcaactg ttgcagcagg
     4021 tgcactggtt atttttgcct tgaagggaat atttttcaat ggatttttaa atagaaataa
     4081 aattgctaaa ttagaaaata gtacaaaaat gagtaaatca gtgccggaag aattgatggc
     4141 gaaagattcg attttaaatg aatctgctga gggtgaatct attgaaacca ggtcatcttc
     4201 taatgatttt acagtaaatg attctacagc aaatgattct gtagcaattg gctctgctgc
     4261 taatgaattg gttgaaaata aatcaactgc aatgataaag ggtgacttta aaggagcagg
     4321 tagttctgaa aaacctattg accaacagga tataggtatt atgttcacag aaggtactgc
     4381 caggtcaaaa gatagcagtg agttgttgaa atatagtgat aaagaagcaa aagaaaaaaa
     4441 cagcatagaa ggatctgaaa ccggagtact tgatgacaag aatgccactg ataaaagcaa
     4501 tattaaaagc tttactgcag atgactttca atctgctaat ggaaaatctg aagcagataa
     4561 agggtctgaa actgatatgg ttcctgaaac taatacaggt tctgaaagcg acataaagct
     4621 tagttcagtt cggggtattg aagattcaaa tgttacaata atgatgagca ctgaaagtgc
     4681 gtgtatgaag aatttacagg agcatgagtt gttatttggg aataacaaaa ttaaagtttc
     4741 tgttttgata gaaggtaata ctgcagagca ggagaaggtc aggtctgtta ttatggaaag
     4801 cggattgctt tcagacgaag aaaaaggaaa tatgatattc aaggaatata atgagaaaag
     4861 tgaggaatcc gctagggagg gtgaaagtac ctttgctctt gtggagctta atatgcgagg
     4921 gattaacttg aaccccttaa ttgatgcaat tatttatagt aagaatgagt taaatgcaca
     4981 ggtagattgt gaagaaccta tttgggatga tggaattaaa ttaaagtatg atgagttaaa
     5041 cacaaagctg cttgaaataa ataaacaaat ttctgcaaca caggaaaaga gcgacagctc
     5101 cagccaatct gacactgaaa agttgatagc tgatagaaat gaaattctga ataatattga
     5161 ggaattgttg cagaatactg aatatattgc tgaaattaga attaacctca ctaggaaata
     5221 gcaagaaata gcaagaatat cagaaatatt agacagtagc catttacggt tgctgttttt
     5281 ttgtgtagaa tattgaaaga cggatactaa attttttgca acatatagta agatgtagat
     5341 atgattacac tgtcaaatat aaattttcgt tattaatata atttggtaga aagggttatt
     5401 ggtatacaaa aaaatggata ataaaaaact aatgttaatt gatggcaaca gtatattgaa
     5461 cagagcattt tatggactca agggctcgca attactggct acatcatacg gcctttatac
     5521 aaatgccgta tatggatttc taaatatact ttttaagtat caggatgaag aacagcccca
     5581 gtatatttgt gtggcatttg atatgaaagc acccactttc agacatgacc aatatgaggg
     5641 atataaggct aacaggaagg gtatgcctga tgaactggct gtgcagatgc ctgttttaaa
     5701 agatgtgctg gatgccatga atataacaag agttgagtat tcaggatatg aagctgacga
     5761 ccttatcggg tggctttcca gatgtggaga agataaagga atggatgtag taatagttac
     5821 aggagataga gattcattgc aactaatatc cgaaaaaact cgcattaagc ttccttctac
     5881 cagattcggg aaaacaaata cggaagaata taatttgagt agtttctatg aaaaatacag
     5941 tatatcccca acccaattta ttgatgttaa gggcttaatg ggagatccct ccgataacat
     6001 tcccggggtc cgggggatag gtgagaaaac tgccctggag cttatcagaa attttggaag
     6061 tatagaggag atatataata acattaataa agtagataag aaaggcgtaa gagagaagct
     6121 tgagacaggg agggacatgg cgtttttaag taggcagctt gccactatag aaagaaaagt
     6181 tccagaggaa ttatgccaga ttgataattt gaaaatacgt gggtatgata ctgaaaggct
     6241 ctatcaatta tttaaaaaac tggagtttaa atctttaatt gaaaagcttg gacttcattc
     6301 cgtggattca aagaataaag atactggtat tacttatatg gaaaaagctg gcgaaaatag
     6361 taatgacgaa aatagtcagg gtgaaaatag ccaagtatca aataatgatc agcagaataa
     6421 cttgaaaaga ataatttata ttaaaaatgc cggtgattta gctgaactgc gaagcagatt
     6481 attgtcctgc aatgaaatag ggatatccca tatattagag aaaactcctg aaaaaggtga
     6541 attggtcggg gaattagcag catttgcttt atcatggaag gaaagcagca gaatttattc
     6601 tgcatatgta gaacttacag aagacataaa tgaggaaaat tttatagatg aatttaaaga
     6661 tatatttttg gatgaatccg taaaaaaaat cgggcacaat ataaaaaatc tcctcttata
     6721 tttgaaatgc agagatataa gcttaaaagg tctggctttt gatactatga tagctgctta
     6781 tattttaaat ccttcaagga atacttatat ggtatcggaa ttaactgcag attatcttaa
     6841 aaaagagatt gagcatttgg aagagttaac aggcaaagga aaaaatcaga tatgctacag
     6901 agaggttcca tgcaaccatc ttgcaagggc tgctacaggc tactctgaag caatacttga
     6961 gcttaagcag gttttagaaa aagaaatccg gtcaaaccaa caggaaatgc tgtattatca
     7021 aattgagctt ccgttggttg aggtgctggc aagcatggaa tattacggat ttaagataaa
     7081 taaggatgaa ctgagtacat tttctgttga gcttgatgaa aagattggca cattaacatc
     7141 agaaatatac agcctggcag gtgaggaatt taacataaac tccactaaac agttaggagc
     7201 aatattattt gataaactcg gacttccggt tataaagaaa acaaagaccg gctattctac
     7261 agatgctgag gtcctggaac agttggcttc aatgcatagc atagtttctt ccattctgga
     7321 gtacaggcag cttatgaagt tgaaatccac atatgttgat ggtctgctta gcgtaataaa
     7381 tgaaagaact gataaaatcc ataccagctt taaccaaact gttgtagtaa caggaagaat
     7441 aagcagtacc gagcccaact tgcaaaatat tccatataaa ttagagatgg gaagaaaaat
     7501 ccgtaaggtt tttgttccat caagcaagga atatattctt acagatgctg actatgcaca
     7561 aattgagcta agagttctgg cgcatatatc cggtgataag agccgtatat ctgcatttat
//
LOCUS       DAOSDD010000216         7609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3554_length_7609_cov_3.229944, whole genome shotgun sequence.
ACCESSION   DAOSDD010000216 DAOSDD010000000
VERSION     DAOSDD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3554_length_7609_cov_3.229944"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /gene="carB"
                     /locus_tag="PK733_15505"
     CDS             complement(<1..2074)
                     /gene="carB"
                     /locus_tag="PK733_15505"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ11978.1"
                     /translation="MPKRTDIKKIMIIGSGPIIIGQACEFDYSGTQACKTLRSLGYQI
                     VLVNSNPATIMTDPGMADATYIEPLNLKRLTDIIAKERPDALLPNLGGQSGLNLCAEL
                     AKAGVLNKYNVEVIGVQVDAIERGEDRTAFKQTMSKLGIGMPQSRSAYSVEEAEEIAM
                     ELGFPVVVRPAYTLGGTGGGRVYNIEELRTVVSRGISASIVGQVLIEEYLTGWEELEV
                     EVVRDSKNQTIAVCFIENVDPLGIHTGDSFCVAPMLTVSQELQNRLQKYAFEIVEAIQ
                     VIGGTNVQFAHDPVTDRIVVIEINPRTSRSSALASKATGFPIASISTMLAVGLTLDEI
                     PYWREGTLDKYTPPSGDYVVVKFPRWAFEKFKGSYDKLGTQMRSVGEVMSIGKNYKEA
                     LQKAIRSLETGRYGLGFAKNYNQKPLNELLELLHETTSERQFIIYEALRKGANIEEIS
                     RLTNIKPWFLEQMKELVVLEEEMLMYKAEKGVELNFPDRLFIQAKKDGFADRYLAKLL
                     GVPEKDIRNRRISLGLSGTWETIPVSGVEDTVYYYSTYNITNDITDDITNDVANNIVN
                     DITIVNDITNDVASYIVNDIVNSVTGTSDNCYTGSAIDGNDGTAANSISNSTDTVVIG
                     RTFCDTLVNTVSSTADNPTTDTVYNTLFNTANNPANNSLVNNSSVNNNTHKVMILGGG
                     PNRIGQGIE"
     gene            complement(2677..4101)
                     /locus_tag="PK733_15510"
     CDS             complement(2677..4101)
                     /locus_tag="PK733_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ11979.1"
                     /translation="MKNIEKHNNTDESIETSKSTSESTYRSTYRSATAYKLMITGFIG
                     IVLIFVSLSGVLLASVYTGNKNTLDEIEQSHQSDKNVNDPQQHQESQHHNTDINKPVL
                     TTGNKEESRHLSDATGQNKIDKDKQDKLNAITEETEEDKIPGKTKYNFDKPVDALAEY
                     SGPVRHIFFHPLIAYPELAFDGDYMAQGFDDWFITVKEFKKILISLHNKNYILVDINY
                     IYEEKEEEGKKTIVKKNMLLPEGKKPLIISIDDNNYYEYMRQNGVVHKLILDSNGNIA
                     DFSITPEGKEIISRDNDIITILDKFVEEHPDFSFQGAKGIIGLTGYEGILGYRTDNPP
                     APNVEEEKKEALKIVKRLKDTGWSFACHGYGHLDAAKISYETLKKDTQRWKDEVEPLI
                     GKTPVYIYPFGSSLLSKDQKFQYLLDEGFNILCAVGNSDYIEYGPNYIAQDRVHIDGV
                     AFRDRMSILSEMFDIDEIIDDARR"
     gene            4391..5218
                     /locus_tag="PK733_15515"
     CDS             4391..5218
                     /locus_tag="PK733_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOJ11980.1"
                     /translation="MQRFMFKTCAITNEIDLNKIAEKCNIRKKYTWEEPLVLKDNILS
                     TILNKKVYDGQAIMLFSFGSIVFINLPSSCINVFLSYLKEYKPEIDIENWERYSDDYE
                     LRAGNVDEIEMTDEYAIVPHIEIAYTELVSVVLAKSVALEKVEEQLEKILDRIESMID
                     RLEKGNLRFSDKELAKATARIIRHEYNSISYIMILDRPDVTWLNSNAAEFYDKMSEFF
                     ELNDRFEILKSKTDILGNVIDGFSSISHSMRSLFIEWVVIVLIVLEVILMIIELIRS"
     gene            5317..6321
                     /locus_tag="PK733_15520"
     CDS             5317..6321
                     /locus_tag="PK733_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOJ11981.1"
                     /translation="MEQQLCNRSCGIKDYRITGSLKARYKMEMKENTFNCFKVNSRLN
                     MKNMASFFNINWPMGKKDYIALKDKQLDTIFKYKTQHKVVYFFEFGCMTFINFDMDEI
                     RIFMEYVESIIGKIDSDMFMKFYEGYAIKVGHNNCFRLYGDGENYTGYSKYIEAIIAV
                     ILAKSTALSWVESQVDILMDEAERIIDNLYKGWLRTNSRKFSRIIARILRFEYESIYN
                     IEILDRTTGLDKKLKFRQVYDDMVGYYELMERFNIVKNKVDNLRRLTRSYANLSYEHH
                     ENRLYIFEIFLLVLFPLSYLFRYLVKYQQLIKIFPVFSSNRLNIFNRAGKDAEKKIDE
                     "
     gene            6473..>7609
                     /locus_tag="PK733_15525"
     CDS             6473..>7609
                     /locus_tag="PK733_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ11982.1"
                     /translation="MYGLVLEGGGAKGAYHIGVSKALVEMGLEIGGVSGTSVGALNGA
                     MIVQGELDKAYEIWQNMNPAQIIKLTDNEEKELDDMEATGEKLSFKIDRLRKIVTERG
                     LDIEPLVKILNKLVSESRVRESSMDFGIVTFDLTNRKPVEIFKEDIPEGKLIDYLIAS
                     ANLPLFKMKTIDGRIYIDGGVYNVLPINLIQDKGYKDIIVVRTFGLGRIKKIDTTGLN
                     VITIAPREGLGPVLDFSAKRARKNLNMGYYDATKVFKSLKGSRYYIKPMNNDDYYIRN
                     LTELKNDRIEKLCHLFGLENAYGKRSLFEYVIPKIADILGVPSSATYEDISIALVEDA
                     ARICEIERFKVYTMDELISEIKKEYIPAKDDFINEIPAFMRGMNI"
BASE COUNT         2453 a         1268 c         1398 g         2490 t
ORIGIN      
        1 attcaatgcc ttgcccgatt cgattgggac ctccgcctaa aatcattact ttatgtgtat
       61 tattattgac tgaactatta ttgactaaac tattgttagc cggattattt gctgtattaa
      121 aaagggtatt atagacggtg tcggtggttg ggttatccgc agtactacta acagtattaa
      181 ctaaagtatc acaaaatgtt ctgccaatga caacagtatc agtgctatta ctgatactgt
      241 tagctgcagt accgtcatta ccatcaatag cactaccggt ataacaatta tcagatgtac
      301 cggtaacact attaacaata tcattaacaa tataactagc aacatcatta gtaatatcat
      361 taacaatagt aatatcatta acaatattat tggcaacatc attagtaata tcatcggtaa
      421 tatcgttggt aatattatat gttgagtagt aatacacagt atcttcaaca ccacttacag
      481 gaatggtttc ccatgttcct gatagtccta aggatatgcg ccggttccgt atatcttttt
      541 ccggtactcc cagaagtttt gccaaataac ggtctgcaaa accatctttt tttgcctgga
      601 taaataatct gtcagggaaa ttaagttcta cccctttttc agctttatac attagcatct
      661 cttcttccaa taccaccagt tctttcattt gttctaaaaa ccatggcttt atattggtta
      721 atctgctgat ttcctcaatg ttagcccctt ttcgtaaggc ttcatatatt ataaactgtc
      781 tttcacttgt agtttcatga agtagttcaa gcaattcatt caaaggcttc tgattataat
      841 ttttggcaaa acccagtcca taacgtcctg tttcgagaga gcgtatagct ttttgaaggg
      901 cttccttata attcttacca atgctcatta cttcacccac cgaacgcatt tgggtgccca
      961 acttatcata ggaaccctta aacttttcaa aagcccacct tggaaatttt actaccacat
     1021 aatcacctga aggtggagta tatttatcca gagtaccttc gcgccagtat ggtatttcat
     1081 ccagagtcaa cccaactgcc agcatggtgg atatggaagc tattggaaat cccgttgctt
     1141 ttgaagccaa tgcagacgac ctggaagttc gtggattaat ctcaattact acaatcctgt
     1201 ctgttacagg gtcatgagca aattggacat tggtgcctcc aattacctga atggcctcaa
     1261 ctatttcaaa cgcatatttt tgaagtctat tttggagttc ctggctgaca gtcagcatag
     1321 gagctacgca aaaagaatcc cccgtatgga ttcccagggg gtcgacattt tcaataaaac
     1381 aaactgcaat cgtttgattt ttcgagtcac gaacaacttc aacctcaagt tcctcccagc
     1441 ctgtcaaata ctcctctata agaacctgac ccactatact tgctgatatt ccacggctta
     1501 ctacggttct tagttcttca atattataaa ctctgcctcc gcctgtacca ccgagtgtgt
     1561 atgcaggtct tacaaccaca ggaaaaccca gttccatggc aatttcctca gcttcttcta
     1621 cactgtaggc agacctgctt tggggcatgc caatacccag tttactcatg gtttgtttaa
     1681 aggccgtcct gtcctcaccc cgttcaattg catcaacctg cacaccaatc acttcaacat
     1741 tgtatttgtt gagtactcct gctttcgcca gttctgcgca taaattaaga ccggattgcc
     1801 cccctaaatt aggtaataaa gcatccggtc tttctttagc aattatgtct gtaagtcttt
     1861 ttaagttaag cggttcaata tatgtcgcat ctgccattcc gggatctgtc atgatggtgg
     1921 cggggttgga atttaccaga acaatttgat aacccaggct tcggagtgtt ttacatgcct
     1981 gcgttcctga ataatcaaac tcgcaggctt gccctattat aatgggacct gaacctatga
     2041 tcattatttt ttttatgtct gtacgtttcg gcatattatc taatcccgtt atacataact
     2101 ataaaactca gtgaaattcc atgtctgctt ccatgtctgc ttcttcatat gactgttaat
     2161 agttgtttaa caattgtttt ataaatatgc taacggtttt cacctacctt ctaaaatatt
     2221 ttacaatttt cccagaaact catttcatga ctttctgggt gataaccata ttatgattgt
     2281 tccccagaaa gccgttttac gacttttcac tgcatgctta cctgcagtga gttgcaacat
     2341 ttttgctacc tggagcaatc taatatgatt atccagcctt cctttgtagg ctgacaaaat
     2401 tcaaggtgac ttaaattttc ccagaaactc atttcatgac tttctgggtg ataaccatat
     2461 tattcatcaa tgttttctag cagtattatc caataatcag tatcttttag caatagttac
     2521 tgcaaaaaaa tcataatcta aaaagcaaat gtttaatacc acccgaatta ttatacacta
     2581 attgtaatta aaattacaat taaaattaca aattatatta atcaaaaaat gtcgaatttt
     2641 gttgaaatta tattttctta ggagattaaa tgttttctac cgtcttgcat catctataat
     2701 ttcatcaata tcaaacattt ctgaaagaat ggacattcta tcgcgaaaag ctactccatc
     2761 tatatgaact ctgtcttgag ctatataatt gggaccgtat tcaatatagt cactatttcc
     2821 aacagcgcat aagatattaa aaccttcgtc caaaagatat tggaactttt gatctttaga
     2881 taagagtgaa ctcccaaagg ggtaaatata aacaggagtt ttacctatca gtggctctac
     2941 ttcatccttc catctttgag tatctttttt cagggtttca tagctgattt ttgctgcatc
     3001 cagatgtccg tatccatggc aggcaaaaga ccagcctgtg tccttcaaac gttttactat
     3061 ttttagagct tccttttttt cttcctctac atttggtgca ggagggttat cggttctgta
     3121 tcctagtatg ccttcataac ctgtcaaacc gataataccc tttgcacctt gaaaggagaa
     3181 atctgggtgt tcttcaacaa atttatccaa tatagttata atatcattat ctctggagat
     3241 tatttcttta ccttcgggtg ttattgagaa atctgcaata ttaccattag aatccaatat
     3301 taatttatgt acaactccgt tctgcctcat atattcatag taattgttgt catcaattga
     3361 tataatcaga ggttttttcc cttccggtaa aagcatattc tttttcacaa tagttttttt
     3421 accttcttct tctttttctt catatatata gtttatgtca acgaggatat agtttttatt
     3481 atgcagagat attaatatct tcttaaattc ttttactgtt ataaaccagt catcaaaacc
     3541 ttgagccata taatcaccat caaaggcaag ttccggatag gcaattaagg ggtgaaagaa
     3601 tatgtgcctt acagggccgc tatattcagc gagcgcatca accggtttat caaaattata
     3661 cttggtttta ccgggaattt tgtcctcttc ggtttcttca gtaattgcgt ttagtttatc
     3721 ttgcttatct ttatctattt tattttgacc tgtagcatca cttaaatgcc gagattcttc
     3781 cttattgcca gtagtcaaca caggtttatt aatgtcagta ttatgatgtt gagattcttg
     3841 atgttgctga gggtcattaa cattcttatc agattgatga gattgctcaa tttcatctag
     3901 agtattttta tttcctgtgt aaacacttgc cagaagaact cctgataaag atacaaaaat
     3961 tagtacaatt cctataaatc cagttatcat aagtttatat gctgttgccg acctatatgt
     4021 tgacctatat gttgactcag aagttgactt agatgtttct attgattcat ctgtattatt
     4081 atgtttttcg atattcttca tttatacatg acctttctaa tttttataaa caatatattt
     4141 ttataaaacc acatgttaat tatgacgtat agagaagaca aaaagttcac attttggaca
     4201 aattgtttca gcttatgatg gcgatataca ttttgctatt ataaatggtt ggaaaatatt
     4261 gattatttgt gtgtttaata tggtataata atttcagaca tagatggatt tattacacca
     4321 ctgattgtac tatttatagt agtggagtaa tagtactgca gtaaataagg gtagaggtgg
     4381 gtaaaaatca atgcaaaggt ttatgtttaa gacatgtgcg ataacaaatg agatagacct
     4441 aaataaaatt gctgaaaaat gcaatatcag aaaaaaatat acatgggaag aaccgttggt
     4501 attaaaggat aatattctta gcactatttt aaataaaaaa gtttacgatg ggcaagcaat
     4561 aatgcttttt tcttttggca gcattgtatt tattaaccta ccctcttctt gtataaatgt
     4621 gtttttaagt tatttgaaag aatacaagcc ggaaattgat attgaaaact gggaaagata
     4681 ttctgatgat tatgaattga gggctggtaa cgttgatgaa attgagatga ctgatgaata
     4741 tgctatagta cctcatattg aaatagcata taccgagctt gtttccgttg ttcttgcaaa
     4801 gtctgtggct cttgaaaagg tagaggaaca actggaaaaa attcttgacc ggatagagag
     4861 catgatagat agacttgaga aaggaaatct gaggtttagt gataaggagc ttgctaaagc
     4921 tactgccaga atcatacggc atgaatataa cagcatcagc tatatcatga tactggatag
     4981 acctgatgtt acatggttga atagcaatgc tgcggaattt tatgacaaga tgtcggaatt
     5041 ttttgagcta aacgacaggt ttgagatttt aaaaagcaag acagatatat tgggaaatgt
     5101 tattgacgga ttctcttcca tcagccactc tatgaggagc ttgtttatag aatgggttgt
     5161 aattgtatta attgtacttg aagttatact tatgattatt gaattgataa ggtcatagct
     5221 gactccgtta accaagtatt gactatcagt atgatcttga agttatactt atgattattg
     5281 aactaataag gccataacca acagcagaac tgaattatgg aacagcagtt atgcaacaga
     5341 agttgtggga ttaaggacta tagaattacg ggtagcttga aagcgagata taagatggaa
     5401 atgaaagaaa atacgtttaa ttgttttaag gtaaattcca ggcttaatat gaagaatatg
     5461 gcctcctttt ttaatattaa ttggcctatg ggtaaaaaag attatattgc attgaaagac
     5521 aaacaacttg atacaatttt taaatataag acacagcata aggtagtcta tttttttgaa
     5581 tttggatgta tgacctttat aaattttgat atggatgaaa tacgaatttt tatggagtat
     5641 gttgagtcta ttatcggtaa aatagatagt gacatgttca tgaagtttta tgaaggatac
     5701 gcaataaaag ttggtcataa taattgcttt agattgtatg gagacggtga gaactatacg
     5761 ggctacagta aatatataga ggctataatt gctgttatac ttgcaaaatc taccgcatta
     5821 tcatgggttg aaagccaggt ggatatcctc atggatgagg ccgagaggat aatagacaac
     5881 ctttataagg gctggttacg taccaacagc aggaaattca gcaggataat agcaaggata
     5941 ttaagatttg aatatgagag catatataat attgaaatac tagatagaac cacggggctc
     6001 gataaaaaac tgaaattcag acaggtatat gatgatatgg taggatacta tgaattaatg
     6061 gaaaggttta atattgtaaa aaacaaagtt gataatctaa gacggcttac caggtcttat
     6121 gctaacttaa gctatgaaca tcatgaaaac agattatata tatttgaaat atttttactg
     6181 gttttgtttc ccttatccta tcttttcagg tatttagtaa agtatcaaca gttgattaaa
     6241 atatttccgg tattttcttc aaaccggctg aatattttca atagggcagg taaagatgca
     6301 gagaaaaaga tagatgaata aaaaatatag ttactgatga atagacagta gatgaaacac
     6361 cggtagatag gattaaacaa atagagcaga tcaggaatga gcaacttgac tggtgacata
     6421 gaaagccaaa cgataggatt aaatatatag agcagttcag gaggtaacaa caatgtacgg
     6481 gcttgtatta gaaggcggtg gtgcaaaagg ggcatatcac attggggtat ccaaagcatt
     6541 ggttgaaatg ggattggaaa ttggcggagt ttcaggaact tcagtaggtg ccttaaatgg
     6601 cgcaatgata gttcaaggag agctggataa agcttatgaa atctggcaga acatgaaccc
     6661 tgcacaaatt ataaagctta cggataatga ggaaaaagag ctggatgata tggaagctac
     6721 aggagaaaag ttgagcttta aaattgacag gttaaggaaa attgtaactg aaagaggtct
     6781 tgatattgaa cctttggtaa aaatattaaa taaattggta agcgaatcca gggttcgcga
     6841 atcctctatg gattttggaa tagtaacatt tgatttgacc aatagaaagc ctgtagaaat
     6901 atttaaagaa gatatacctg aaggtaaatt aatagactac cttatagcaa gtgcaaatct
     6961 tccattgttt aagatgaaaa caatagacgg gagaatatac attgatggag gagtttataa
     7021 tgtgctccct atcaatctta ttcaagacaa agggtataaa gatataattg tagtaagaac
     7081 ctttggactg ggacgtataa agaaaattga tactacaggg ttaaatgtaa ttacaatagc
     7141 accaagagaa ggccttggcc cggtattaga cttcagcgcg aaaagagcaa ggaaaaatct
     7201 gaatatgggt tattatgatg ccacgaaagt gtttaaaagc ttaaagggta gtaggtatta
     7261 cattaaacca atgaacaatg atgattacta cataagaaat ttgacagagc tgaaaaacga
     7321 taggattgaa aaactttgcc acttgtttgg attggagaat gcatatggta agagatcgct
     7381 ttttgaatat gtgataccta aaatagcaga tatactggga gttccttcaa gtgctaccta
     7441 tgaagatata tcaatcgccc tggttgaaga tgctgcacga atttgcgaaa tagagagatt
     7501 taaggtatat accatggatg agttaatatc agagataaaa aaagagtata tacctgcaaa
     7561 agatgatttt attaatgaaa tacctgcatt catgaggggt atgaatatt
//
LOCUS       DAOSDD010000217         7557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3577_length_7557_cov_3.490536, whole genome shotgun sequence.
ACCESSION   DAOSDD010000217 DAOSDD010000000
VERSION     DAOSDD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3577_length_7557_cov_3.490536"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /gene="nusA"
                     /locus_tag="PK733_15530"
     CDS             <1..458
                     /gene="nusA"
                     /locus_tag="PK733_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255599.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HOJ11983.1"
                     /translation="SREPGSRTKIAVFSKDENVDPVGACVGHKGIRVQAIVDELRGEK
                     IDIIKWSSNPGEFISCSLSPAKVLRVDVNEKEKGARVIVPDYQLSLAIGKEGQNARLS
                     AKLTGWKIDIKSESQLRAEIEQQLLNFNANYKDAQEDEEDKEDKENDEE"
     gene            491..763
                     /locus_tag="PK733_15535"
     CDS             491..763
                     /locus_tag="PK733_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOJ11984.1"
                     /translation="MKQKKIPMRMCLGCRESKPKKDLIRVVKNKENEISVDLTGKMHG
                     RGAYICRGKLCLEKAIKGKRFEKSFEMTVSPEVFDILNKQMEEIDE"
     gene            756..1082
                     /locus_tag="PK733_15540"
     CDS             756..1082
                     /locus_tag="PK733_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOJ11985.1"
                     /translation="MNNRIYPFLGLAAKAGKLISGEEACERAVKSGKVCLILVAEDAA
                     NNMKMRFENMCKYREVDIRIFGERELLGKFTGKDMRVVIGILQKEFAKQLLQMIDNQK
                     LGIGGV"
     gene            1085..4246
                     /gene="infB"
                     /locus_tag="PK733_15545"
     CDS             1085..4246
                     /gene="infB"
                     /locus_tag="PK733_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ11986.1"
                     /translation="MEKVRIYELAKELNTTSKRLIEKLAEIDINVKNHMSYLDVNELE
                     ALYKHIGVVSYDEEKKDEDRKKSSTSTPRSKMEQKKPEVKDSNYKSAPRIIRKTEIVL
                     DEKVNDKYKEDSNRKDGQHGRNRGESRGGRRYGGIKVNTAGTGLRAGYVRDTGPSIIK
                     KDKLPRTDKVQEKPQDTRKSIIESASDDVKMKQAIEEPVTQAIEEPVTQPVEQAVREP
                     AKELISNENEEKKTGGKKISVLPKEEIKKVNVEKKKTENKTGEKEAQKPAQKEQKKVT
                     DKYISRQEYKETLRNKDQQAKTNFTGEKSWVRTGEDMKTMENGKKNEEKHEARSDSYK
                     DKKKAKSTGYTAAKHDRHEALKPDFLEIPKPDIRIEPEESKKFSYKNKEADKDSKREQ
                     KRELPRAQISDSKKKKFKTNEIVIDEKRNISQLITDDGIIDNKYEERKGKRPVRAPKR
                     KKEDKNIEIESNASIIARRSTLLSISIPEEITVKELAESLKKTSADVIKKLMNMGIMA
                     TLNQVIDYDTAAIVADEFGVKAEKEIIINEEDILFDNEEDNIADLKARPPVVVVMGHV
                     DHGKTSLLDAIRKTNVIKSEEGGITQHIGAYMVKINNRNITFLDTPGHEAFTAMRARG
                     AQATDIAILVVAADDGIMPQTIEAINHAKAADVAIIVAINKIDKPGANPERIKQQLTE
                     HGLVPEEWGGDVIVVPVSALKGENIDLLLEMVLLTADMLELKANPKKQAKGTVIEAKL
                     DKNRGPIATLLVQRGTLSLGNSILAGTILGRIKAMTDDKGQNIKDAAPSTPVEVLGFS
                     DVPESGEIFYAIEDERLAKSLIEKRKQKHREEQLKSSSRVTLEDLFSQIQEGKVKELN
                     IIIKADVQGSVEAVKQSMEKLSNDEVKINVIHGGAGGITETDVKLAEVSNAIIIGFNV
                     RPGINVSETAKSVNVDIRLYRIIYDAIEDIEAAMKGMLEPKLEEVIHGHAEVRQLFKA
                     SGIGVIGGSYVLDGKITRNSGVRVVRDSIVVYEGKLASLKRFKDDIREVSQGYECGIL
                     IEKFNDIKEGDIVEAYAMEEVQE"
     gene            4346..4693
                     /gene="rbfA"
                     /locus_tag="PK733_15550"
     CDS             4346..4693
                     /gene="rbfA"
                     /locus_tag="PK733_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOJ11987.1"
                     /translation="MAERIYRISEEIKREISNIIQNELKDPRLPEFVSVVSVNTTRDL
                     RYARVYVSVFGNDEKKKSAMEGLRNAAGFIRREIGQRIKLRYTPELQFELDNSIEHGI
                     YINKLINETVKGD"
     gene            4715..5674
                     /locus_tag="PK733_15555"
     CDS             4715..5674
                     /locus_tag="PK733_15555"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOJ11988.1"
                     /translation="MILDEIIDVISISRSISVLPHISADGDALGSGLALALALSRTNK
                     NVAVYLEENIPRLYSFLPGQEMVEVFPESVEIPDTAIAIDTGDIERLGKRIELFKKAN
                     NRINIDHHYTNTKFADLNYVCVNSSAVGEIIYQIINMMGFSLDKDISTCLYVAITTVT
                     GGFRYENTTYKTHLIASDLLKSGINIQEISSKVFDSISKEKVKLTGAAISSLELLEDE
                     RIAFITVTERMMEETGALEEECEGIINLGRNIYGVEIAALFREKQGEVKVSFRSNSDV
                     DVSTIANRYSGGGHKKAAGCTIKGTLHEVKKKILNDMSDILKR"
     gene            5679..6659
                     /gene="truB"
                     /locus_tag="PK733_15560"
     CDS             5679..6659
                     /gene="truB"
                     /locus_tag="PK733_15560"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ11989.1"
                     /translation="MGVYSINGLLNVLKPPGMTSFDTVAYLRRILKEKKIGHAGTLDP
                     DAAGVLPICTGKATKVIEYIMDMEKVYRAELILGVSTDTQDSSGNVIAVNEVTASEQQ
                     IISAIKSFMGEIYQTPPMYSAIRLEGRRLYELAREGKSVERKPRRVEIHSIDVIKIDK
                     EKVLFDVTCSKGTYIRTLCLDIGDRLGCGGHMSFLIRKRTGIFDIGSALTLEEILKAN
                     NDGKICGMFLKVDEVFKEYKSFHLNEHSEKLFKNGGFIYINNINIINKTNNENIIDEI
                     SNVNIIDKNHYDFSSGLLVRVYNEKKDFLALGQVIVTNGSCYLKPKKMFV"
     gene            6687..>7557
                     /locus_tag="PK733_15565"
     CDS             6687..>7557
                     /locus_tag="PK733_15565"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ11990.1"
                     /translation="MEVMRGIKNTLLTKPLGIGLGNFDGLHIGHITLINALINESRLN
                     DLHSMIYTFAKHPENILKKELITPLLITVEKKAELLSSLPLDYLYYEDFDESFSKMQP
                     EDFVKDILVGNFNIKLAMAGFDYTFGYRGRGDAKLLKELGEKHNFRVIIIPPIKINDE
                     VISSTLIREYLTSGDMEKTFYFLGRHYSITGLVEGGHRMGSELGFPTANINPEEYLLL
                     PAHGVYITKTLLDGKLHKSVTNIGMAPTIKTGRTRVNIETYILDFNGDIYQKNIEVFF
                     IKKIRDEKKFGSKE"
BASE COUNT         2886 a          987 c         1732 g         1952 t
ORIGIN      
        1 tttcacgtga gcctgggtca aggacaaaaa ttgctgtttt ttccaaagat gagaatgtag
       61 acccggtagg tgcttgtgta ggtcataagg gaataagagt acaagctatt gttgatgaat
      121 tgcggggaga aaagatcgat attattaaat ggagcagtaa ccctggagaa tttatatcat
      181 gcagcttaag tcctgcgaag gttttaaggg ttgatgttaa tgaaaaggaa aagggtgcaa
      241 gagtaatagt acccgactat caattgtctc tggccatagg caaggaagga caaaatgcgc
      301 gcttatctgc aaagcttaca ggttggaaaa ttgacattaa gagcgaatct caattgagag
      361 ctgaaataga acagcaacta ttaaacttta acgcaaatta caaggatgca caagaggacg
      421 aagaggacaa agaggacaaa gagaacgatg aagaataact ttgcgtaagt tatcgggagt
      481 gtggtacaat atgaaacaga aaaagatacc catgcgtatg tgtcttggat gcagagaatc
      541 aaagcccaaa aaagatttga taagagtagt caagaataag gaaaatgaaa tatctgttga
      601 tcttacgggt aagatgcatg gccgcggtgc atatatatgc cggggtaaat tgtgccttga
      661 aaaagcaata aaaggaaaaa gatttgaaaa aagttttgaa atgacggtaa gtccggaagt
      721 atttgatata ttaaacaaac aaatggagga gattgatgaa taatagaata tatcctttcc
      781 ttggattagc tgcaaaagca ggtaaactta tatctggaga agaagcgtgt gaaagagctg
      841 ttaaaagtgg taaagtctgt ttaatattag tagctgagga tgctgcaaat aatatgaaaa
      901 tgcgctttga aaatatgtgt aaatacaggg aagttgatat tagaatattt ggtgagagag
      961 aattattagg taaatttaca gggaaggata tgagggtagt tattggtatt ttacagaagg
     1021 agtttgcaaa acagcttttg caaatgatag ataatcaaaa attaggaatt gggggtgtat
     1081 aaacatggaa aaagttagga tttatgaact tgctaaagaa ctaaatacaa caagtaaaag
     1141 gctcattgaa aaactggctg aaattgatat aaatgtaaaa aatcatatga gctatcttga
     1201 tgtaaacgaa ttggaggctt tatataaaca tataggtgta gtttcttatg atgaagaaaa
     1261 aaaggatgag gataggaaaa agtcttctac ctccacaccc cgaagcaaaa tggagcaaaa
     1321 gaagcctgaa gtgaaggact ccaactataa aagtgcgcca aggataataa ggaagaccga
     1381 aattgtactt gatgaaaaag ttaatgataa atataaagaa gatagtaacc gtaaagatgg
     1441 gcagcatggg cgtaatagag gtgaatccag gggaggaaga aggtatggtg gtataaaagt
     1501 caacactgca gggacaggtt tgagggcagg ttatgtaagg gataccgggc cttcaataat
     1561 aaagaaagac aaactgccaa ggacggataa agtacaggaa aaaccccagg atacccgcaa
     1621 atcaatcata gaatctgcaa gtgatgatgt aaaaatgaaa caggcaattg aagaaccagt
     1681 tacacaagca attgaagaac cagttacaca accagttgaa caagcagtta gagaaccggc
     1741 taaagagctg atttctaatg aaaacgaaga aaaaaagact ggaggtaaaa agattagtgt
     1801 tttaccaaaa gaagagataa agaaagtgaa tgttgaaaaa aagaaaacag aaaataaaac
     1861 aggagagaag gaagctcaaa aaccagccca aaaagaacag aagaaagtaa cagataagta
     1921 tattagcaga caagaataca aagaaacgtt gcgtaataag gaccaacagg caaaaaccaa
     1981 ttttacaggc gaaaaaagct gggttaggac cggggaagac atgaaaacca tggaaaatgg
     2041 taagaaaaat gaagaaaaac atgaagcaag gtctgatagt tataaggaca aaaagaaggc
     2101 aaagagtaca ggctacacag ctgccaagca tgacaggcat gaagcgttga agcctgactt
     2161 cctggaaata ccaaagcctg acattaggat tgaacctgaa gaatcaaaga agtttagtta
     2221 taaaaacaaa gaagctgata aggatagtaa gcgtgagcag aagagagaat tgccaagagc
     2281 acaaatttct gatagcaaga agaagaaatt taaaacaaat gaaattgtaa ttgatgaaaa
     2341 aaggaatatt tcacaattaa ttacagatga tggaattatt gataataaat atgaggaacg
     2401 aaaaggtaaa agacctgtaa gagctcctaa acgcaaaaaa gaagataaga atattgaaat
     2461 tgaatccaat gcttccataa ttgcacggag atctacgtta ttatcaataa gtattcctga
     2521 ggaaattact gtcaaggaac ttgccgagag tttgaaaaaa acttctgcag atgttattaa
     2581 gaagttgatg aatatgggca ttatggctac tttgaaccag gtgatagatt atgatactgc
     2641 agctatagtt gccgatgaat ttggagttaa agcggaaaaa gaaattatta ttaatgaaga
     2701 agatatttta tttgataatg aagaagataa tattgcagat ttgaaggcaa gaccgcctgt
     2761 ggttgttgtt atggggcatg tagaccatgg gaaaacatcc cttcttgatg caatcagaaa
     2821 aactaatgtt attaaaagcg aagaaggtgg cataacacag cacataggtg cttatatggt
     2881 aaaaataaac aacagaaata ttacattcct tgatactccc ggtcatgaag cattcactgc
     2941 catgagagcc agaggagcgc aggcaacgga tatagctata cttgtagttg cagcggatga
     3001 tggtataatg ccgcagacaa tagaagccat aaaccatgca aaggcagctg atgtagctat
     3061 aattgtggca ataaacaaaa tcgacaaacc aggggctaac ccggaaagga taaaacaaca
     3121 actgacagaa cacggtcttg tacctgaaga atggggaggc gatgttattg ttgttcctgt
     3181 atcagcttta aagggagaga atattgatct gcttctggaa atggtattat tgactgcaga
     3241 tatgctggag cttaaagcaa accctaaaaa gcaggctaaa ggtactgtaa tagaagcaaa
     3301 acttgataaa aacagaggac ctattgctac attgttagtt caaagaggaa cactttcttt
     3361 aggcaattca atattagcag gtacaatttt gggtaggata aaggctatga cagatgataa
     3421 aggccaaaat ataaaagatg cagcaccttc aactcctgtt gaagtactgg gtttttctga
     3481 cgtacctgaa agtggggaaa tattttatgc aatagaagat gaaaggctag ctaaaagctt
     3541 gatagaaaag agaaagcaaa aacatagaga agaacagcta aaatcaagct caagggttac
     3601 tcttgaagat ttgttcagcc agattcagga aggcaaggtt aaggagctta atattataat
     3661 taaagccgat gtacaaggat ctgttgaggc tgtaaagcaa tctatggaaa agcttagcaa
     3721 tgatgaagtt aaaattaatg ttatacatgg cggagcagga ggcattactg aaactgatgt
     3781 taagctggcg gaagtatcca atgctattat tattggtttt aatgtaagac ctggaataaa
     3841 tgtatcggaa actgctaaaa gtgtgaatgt ggatatcaga ctatacagaa taatatatga
     3901 tgctattgaa gatattgaag cagctatgaa aggcatgtta gaacccaaac tggaggaggt
     3961 aatccatgga catgctgaag taaggcagtt gttcaaagca tctggtatag gcgttatagg
     4021 aggatcttat gtccttgatg gcaagataac cagaaactcc ggtgtacgtg tagtaaggga
     4081 cagtattgtg gtttatgagg gaaaacttgc atcgctaaaa aggttcaaag atgatataag
     4141 ggaggtttca caaggctatg aatgtggaat acttatagag aagtttaacg atataaaaga
     4201 aggagatatt gttgaggctt atgctatgga ggaagtgcaa gaatgagttt ttatattttt
     4261 acactaaaat ccgggttaat acagcaggtt aatgcagttg aataatacag tagaataaag
     4321 aataggctca aggagtgatt tactgatggc tgaaagaata tatagaatat ctgaagaaat
     4381 aaaacgggag atcagcaata ttattcagaa tgaattgaaa gacccaaggc ttccagaatt
     4441 cgtaagtgta gtgtcagtta atactaccag ggatttaaga tatgcaagag tatatgttag
     4501 cgtatttgga aatgacgaaa aaaagaaaag tgctatggaa ggtttgagga atgcagcagg
     4561 gtttatcaga agagaaattg ggcaaaggat taagctgaga tatacgcctg aattacagtt
     4621 tgaactggat aattctattg aacatggaat ttatattaac aagctgataa atgaaacggt
     4681 aaaaggggat tgaagcaaac aggatggtag aattatgata ttagatgaaa taatagatgt
     4741 tataagcata tcaaggagca tttctgtact tccccatatt tcagctgacg gtgatgcgtt
     4801 ggggtcagga ctggcactgg ctctggcgtt gagccgtaca aataaaaatg tggcagtata
     4861 tcttgaagaa aatatccccc gactctatag ttttctacct ggacaggaaa tggttgaggt
     4921 ttttccggag agtgttgaaa tacctgatac ggctatagct atagataccg gagatattga
     4981 aagactgggt aagagaattg aattatttaa aaaagcaaac aataggataa atattgacca
     5041 tcactataca aatactaaat ttgccgattt aaactatgtt tgtgtaaatt catctgctgt
     5101 gggggaaata atataccaga taataaatat gatggggttt agtttggaca aagatatttc
     5161 aacatgcctc tatgttgcaa taacaactgt cacaggcgga ttcagatatg aaaacacgac
     5221 atataagact cacttaatag cttcagactt attaaaaagc ggtattaata tacaggaaat
     5281 atcttcaaaa gtatttgatt caatttcaaa agaaaaagta aaattaacgg gtgctgcaat
     5341 aagctctctt gaacttttag aagatgagcg gatagcattt attactgtaa cggaaagaat
     5401 gatggaagag actggtgccc ttgaagaaga gtgtgagggc attataaact taggaagaaa
     5461 catatatgga gttgagattg cagccttatt cagggaaaaa caaggggaag taaaagtcag
     5521 cttcagatca aattctgatg ttgatgtctc aactattgca aacagatatt ccggtggtgg
     5581 gcataaaaaa gccgccggtt gtacaataaa aggtacgttg catgaagtaa aaaagaaaat
     5641 actgaatgat atgtcagata ttttaaaaag atgaggaaat gggggtttac agtattaatg
     5701 gcctgttaaa tgttttaaaa ccaccaggta tgacatcttt tgatacagtt gcatacttga
     5761 ggagaatatt aaaggagaaa aaaattggac atgccggtac gctggaccct gatgcagcgg
     5821 gagttttacc aatatgtacg ggtaaggcca ccaaagtcat agaatatata atggatatgg
     5881 agaaagtgta tagagctgaa cttattcttg gagtctctac agacacacag gattcttcag
     5941 gaaatgttat tgccgtaaat gaagttactg cgtctgaaca acaaataatc agtgctataa
     6001 agagttttat gggtgaaata tatcagacgc ctcctatgta ttctgctatt agattagaag
     6061 gcagaaggct ttatgaactt gcaagagaag gaaaaagcgt ggaacgaaag ccaagaagag
     6121 ttgagataca ttcaatagat gtaataaaga ttgataaaga aaaagttctt tttgatgtaa
     6181 cctgttcaaa ggggacatat ataaggacat tatgcttgga tattggagat agactgggtt
     6241 gtggaggaca tatgtcgttt cttatacgaa agaggacagg aatatttgat attggatctg
     6301 cacttaccct ggaagaaatt ctgaaagcaa acaatgatgg taaaatatgc gggatgtttt
     6361 taaaggtcga tgaagttttt aaagagtaca aaagttttca tttgaacgaa cacagtgaaa
     6421 aattatttaa gaatggtggg tttatttaca taaataacat aaatattatt aataaaacga
     6481 ataatgaaaa tattattgat gaaataagta atgtgaatat tatagataaa aaccattacg
     6541 attttagtag tggtttactg gtaagggtat ataatgaaaa aaaagatttt cttgctcttg
     6601 gccaggtgat agtaacaaat gggagttgct atttgaaacc taaaaaaatg tttgtttgac
     6661 gacataatgt gatgaggaga aaacctatgg aggtcatgcg tggtattaaa aatacattgc
     6721 tcacaaaacc gctgggtatt ggattgggta attttgacgg actgcatatt ggacacataa
     6781 ccttaataaa cgcactaata aatgagagca ggcttaatga tttacattca atgatctata
     6841 cctttgcaaa acatccggaa aatattttaa agaaagaatt aattacacca ttattgataa
     6901 cagtagaaaa aaaagcagaa ttgttaagca gcctgcccct tgattattta tattacgaag
     6961 actttgacga atcattttcc aaaatgcaac ctgaggattt tgtgaaagat attttggtag
     7021 gcaactttaa cataaagctt gctatggcag ggtttgatta tacttttggc tataggggcc
     7081 gaggtgatgc caaattgttg aaagaattag gagaaaaaca caatttcaga gttataataa
     7141 ttccgcctat taaaataaat gacgaggtta taagcagtac acttatccgt gagtatttga
     7201 caagtggtga tatggaaaag accttttact ttcttggaag gcattactcc ataacaggat
     7261 tagttgaggg tggacaccgt atgggaagcg agcttggatt ccctacagct aatattaatc
     7321 cggaagaata tcttctcttg cctgctcatg gagtctacat aactaaaaca cttctggatg
     7381 gaaaactgca taaaagcgtt acaaatattg gaatggcacc tacaataaag actggaagaa
     7441 cccgggtaaa tatagagaca tatatattag attttaatgg agatatttat caaaaaaata
     7501 ttgaagtctt ttttattaaa aaaatccggg atgaaaaaaa gttcggtagt aaggagg
//
LOCUS       DAOSDD010000218         7473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3632_length_7473_cov_4.956053, whole genome shotgun sequence.
ACCESSION   DAOSDD010000218 DAOSDD010000000
VERSION     DAOSDD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_7473_cov_4.956053"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1398)
                     /locus_tag="PK733_15570"
     CDS             complement(112..1398)
                     /locus_tag="PK733_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ11991.1"
                     /translation="MKFDLLHPADQIVMIMERIYGYGMTTTSGGNLSVLDDNGDIWIT
                     PSGIDKGSLTTKDIIRIKSDGTMEGIHKPSVELPFHKEIYQKRPDIKAIIHAHPPALI
                     SFSIVRKIPNTRLIPNAHIICGSVGMAEYGLPGSPDLGSKIAAEFENGHNTVMLENHG
                     VVVGSENLFKAFMAFETLDFCARLEIKANRIGKPIALTEKHIEISRNKQNVKMDEFIP
                     QSYSSEEKAARQEMCRLIHRAYDQKLFTSTQGTFSQRLNNDSFIITPYDADRKYLDID
                     DIARIDMDWKEAGKNPSRSVMLHKYIYEMHSNVSSVIIAHPPNIMAFAVTEEKFETKT
                     IPESYIMLRDIPKLPFGASFMQPKMVAEAFSKETPILLIENDCIIVTGSSLLNAYDRL
                     EVAEYSAKAIISSKVLGNVIEINKEQVAEIEEAFKL"
     gene            1837..3663
                     /locus_tag="PK733_15575"
     CDS             1837..3663
                     /locus_tag="PK733_15575"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOJ11992.1"
                     /translation="MQIIHLRGDFLVNSNKKLQTWVDEMAELCQPDKVYWCDGSEEEN
                     NHLLEQMVKSGAATPLNPEKRPGCYAFRSDPSDVARVENRTFISSVKQEDAGPTNNWI
                     DPKELKPTMTELYRGCMKGRTMYVIPFSMGPIGSPISKIGVELTDSPYVVANMRIMTR
                     MGTKVLEALGEDGEFVPCLHSVGYPLEEGQIDLEWPCAPIEKKYISHFPEERTIWSYG
                     SGYGGNALLGKKCFALRIASVLARDEGWLAEHMLILKLTDPKGNVKYITGAFPSACGK
                     TNLAMLVPTIPGWKVETIGDDIAWMKFGADGRLYAINPEAGFFGVAPGTSMDSNPNAM
                     LSTRKNTIFTNVALTDDGDVWWEGINGKAPSHLIDWKGKDWSPDSGTPAAHPNARFTA
                     PANQCPAIAPEWEDPAGVPISAMLIGGRRPSTIPLVHESFDWNHGIFMGSIMGSEITA
                     AAISDKIGQVRRDPFAMLPFIGYHVCDYLQHWLDIGEKTDADKLPKIFYVNWFRKDKN
                     GKWLWPGFGDNSRVLKWIFERTSGEGKAVETPIGYMPATDAVDVEGLDVPESDMKELL
                     SVNKEEWLREVDSIRKHYASYGERLPKELASQLDALEARLKK"
     gene            3996..4961
                     /locus_tag="PK733_15580"
     CDS             3996..4961
                     /locus_tag="PK733_15580"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOJ11993.1"
                     /translation="MLKTMFKRYSVVLITFILLFVFLTGCGSSSSKSDSAVMETSRGG
                     GVSYNSASNSKVTLTSKQSNYYDGGQSAADTNSNSQSAQDAKEDIKKVIKNGDISVEV
                     KDVDIAYEGIMKIVESLGGEEFNKNYTISGEYKRMELVLKIPPEKLNLFEQKIKEYVG
                     EGKVRNINIRSRDITSEYYDYTARLESYQASRDQMRELLKKAQNIDETLRIQQELTRL
                     QAEIDSMQGQVKMWDKLIDMATINLYIDEEDNPFKKTTAVSWKFNSPAEIWSTMKNGF
                     VGVVNTIYSLLIWILIAVVSLSPVIAVASIVIYVIFFVKKKRKQK"
     gene            5087..6136
                     /gene="ytvI"
                     /locus_tag="PK733_15585"
     CDS             5087..6136
                     /gene="ytvI"
                     /locus_tag="PK733_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOJ11994.1"
                     /translation="MFNEKFIKSLKNFLIFLLLYTAVFILVSKTMSYTFPFIAAFCIA
                     FLMQPVTRFFKEKLKLNKNIPSLLSSALVFLVLFVLVTLLFYKIIVEAKQLLLNIPNI
                     NMELIMEPIRKIINDIGLYFKDIDPDFIEKNSRQISEIISNILSVLGKTLNSFLTVAL
                     SIPMWITVIFIVILSTYFFSRDMAAIKARAVSIFSEKGRDKFILVWYEGVKMLSHYIK
                     AYLFIYTLTFIQTLAGFSILRIKYAVILSIVCAVADVLPVLGIGIIYLPLSAIYFIAG
                     NYVAAIGIIILYIIISVVRQIVEPKIVSTSLGIHPVIALATIFIGLKAYGFLGMIYFT
                     FLMIFYKILKTAKVL"
     gene            6410..7246
                     /locus_tag="PK733_15590"
     CDS             6410..7246
                     /locus_tag="PK733_15590"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ11995.1"
                     /translation="MSDNIKFSAAVALSGVIFAPMGMLPEYILLYLLGTLLYIISSEY
                     DKTKMKTSEENDNLRAELDTLYMQINRNEEYQRQMIHLSQLEERNFIAQKIHDSTGHT
                     IAGALIQLEAAALLIERDGKKAQGIIKNVTSILREGMENIRRTLRNIKPPSEQLGISK
                     MKTVLNEFSLKHDVKTEFSYNGNIDKISFIQWKIIYENLIEALTNSLKYSQCTIINVT
                     LQVFHKVVKFEVKDNGHGAAALNKGMGLKGIEERCANIGGKVIIDGSKGFSIITLLPM
                     EN"
BASE COUNT         2466 a         1191 c         1433 g         2383 t
ORIGIN      
        1 aggaatatct gttacttgag agaagagttt cccctgccca gctacagtac ctacactaat
       61 gcttcttcca ataatttctt caaaaataaa atatgcatcc cctcttaggc tttataattt
      121 aaaagcctct tcaatctcag ctacctgctc tttattaatt tctattacat tacctagcac
      181 ttttgaggat attatagctt ttgcactata ttctgcaact tcaagcctgt cataagcatt
      241 tagcagagag cttcctgtca ctataatgca atcattttca ataagcaaaa ttggtgtttc
      301 tttcgagaaa gcttctgcta ccatcttggg ctgcataaaa ctagccccaa aaggcagctt
      361 ggggatatca cgcaacataa tgtaactttc cggaatagtt tttgtttcaa acttttcctc
      421 tgttactgca aaggccatta tgtttggcgg gtgtgctatt attactgaac taacattgga
      481 atgcatttcg taaatatact tgtgaagcat gactgaacga cttgggtttt tgccagcttc
      541 tttccaatcc atatcaatcc ttgcgatatc atcaatgtcc aggtatttcc tgtcagcatc
      601 ataaggtgta attataaaag aatcattatt tagccgctga gagaatgtcc cctgggtact
      661 tgtaaaaagc ttctggtcgt atgcccggtg aataagcctg cacatttcct gacgggctgc
      721 tttctcttca ctgctgtaac tttggggaat aaactcatcc atttttacat tttgcttatt
      781 tctggagatc tcaatgtgtt tttccgttaa agcaatcggt ttaccaatac gattagcttt
      841 tatttcgagc cttgcacaaa agtccagagt ttcaaatgcc ataaatgcct taaacaaatt
      901 ttcgcttcca acaacaacac catggttttc aagcattaca gtattatgtc cattttcaaa
      961 ttccgccgca attttgctgc caaggtccgg acttcctgga agtccatact cagccatacc
     1021 tacagaaccg catataatgt gggcattggg aatcagcctt gtgttgggaa tttttcgcac
     1081 aatactaaag gatattaatg ccggagggtg ggcatgaata attgccttaa tgtccggcct
     1141 tttctggtat atttctttat ggaaaggtaa ttctactgaa ggcttatgaa tgccctccat
     1201 agtaccatcg gatttaatcc ttataatatc tttggtagta agggaaccct tatctatacc
     1261 gctgggtgta atccatatat ctccattatc atccagaact gaaaggtttc ctccagatgt
     1321 agtagtcata ccataaccat aaatcctttc catgatcata actatctggt ctgcaggatg
     1381 taacagatcg aatttcaata ctttagcctc cttaacaaaa cttgcttatt attatataga
     1441 aaatacagtt gttaatatta atacctaaat aaacataaat acctaaatgc taatctaata
     1501 ctcaaaatac tcaaatattt atactgtaaa tactaatagt gtaaatatta atactgtata
     1561 aaataccaat atatcaataa gtattctact cctgatagat aaataattca agatggtaga
     1621 aaagcaaata taaaaacaaa tataaaaaca attgttgaat ttattcttag aacgtgatat
     1681 tattttaaca atgaaaaaat ggatgtatag gctgtttttt tagaaactct aataaaaaac
     1741 agattatatc cattacttaa ataaatgcat ttataaaatt gtattagtaa acaataaata
     1801 ttatacctga atcaggtttt gatatttaat acttaaatgc aaataattca cttgaggggg
     1861 gattttttag tgaattctaa caagaaatta caaacctggg tagatgaaat ggctgaattg
     1921 tgccagcctg ataaggttta ttggtgcgat ggttcagagg aggaaaataa ccacctgctg
     1981 gaacagatgg ttaagtcagg agctgcaaca cctttaaatc ctgaaaaacg tcccggatgt
     2041 tatgcattcc gcagtgatcc atctgatgta gccagagtag aaaatcgtac ttttatttct
     2101 tctgtaaaac aagaagatgc aggtccgaca aataactgga ttgatccaaa agagcttaag
     2161 cctacaatga cagaattata tagaggctgc atgaagggca gaactatgta tgttattcca
     2221 ttctctatgg gacctatagg ttcacctatt tcaaaaatag gcgtagaatt aactgacagt
     2281 ccatatgttg ttgctaacat gcgtattatg actcgtatgg gtactaaagt acttgaagct
     2341 cttggggaag atggagaatt tgttccctgc ctgcattcag taggatatcc gctggaagaa
     2401 ggacagatag atcttgaatg gccttgtgct cctattgaga aaaagtacat cagccatttc
     2461 ccagaggaaa ggactatctg gtcctatggt tctggatatg gtggaaatgc actcttgggc
     2521 aaaaaatgtt ttgcactgcg tatagcttca gtgttggcac gcgatgaagg ttggttagct
     2581 gagcatatgc tcattttaaa attaactgac cctaagggaa atgtaaaata tattacaggg
     2641 gctttcccga gtgcatgtgg aaagactaac ctggcaatgc ttgttccaac tattccaggt
     2701 tggaaagttg aaacaatagg tgatgatata gcatggatga aatttggtgc tgatggacgt
     2761 ttatatgcaa taaatccgga agcaggattc tttggtgttg cccctggtac atctatggat
     2821 tcaaatccca acgctatgtt aagcaccaga aaaaatacca tatttactaa tgtagcttta
     2881 actgatgatg gtgatgtttg gtgggaaggg ataaacggca aagcaccttc acatcttata
     2941 gactggaagg gaaaagactg gtcacctgat tcaggtacgc ccgcagctca tcctaatgcc
     3001 cgttttacag cacctgcaaa tcaatgtccg gcaattgcac cggaatggga agatcctgca
     3061 ggagtcccaa tatcggccat gttaattggt gggcgtcgtc caagcactat tccgttggtt
     3121 catgaaagct tcgactggaa tcatggcata tttatgggtt caatcatggg ttctgaaatt
     3181 actgctgcag caatatctga taaaattggt caagtacgcc gtgatccatt cgctatgctg
     3241 cctttcatag gttaccatgt atgtgattac ttacagcatt ggctggacat tggagagaaa
     3301 actgatgcag ataagttacc taaaatcttt tatgtaaact ggttccgtaa agataaaaat
     3361 ggcaaatggc tctggcctgg attcggagat aatagccgtg tactcaaatg gatttttgaa
     3421 agaacatctg gtgaaggcaa ggcagttgaa acacctattg gttatatgcc agcaacagat
     3481 gctgttgatg ttgaaggcct tgatgttcca gagtctgata tgaaagagct tctatcagtt
     3541 aataaagaag aatggttacg cgaagtggat tcaattagaa aacattatgc cagctatggc
     3601 gaacgtcttc caaaagaatt agcttcacaa cttgatgcat tggaagctag attgaaaaaa
     3661 taatatttga aaaataataa tagggacgct taagcgtccc tatatttttt aatgaatagg
     3721 aaagtgcaaa ttactttagt gtacactact atcccacata attccttatg aagacaggaa
     3781 acttatttcc taactcctca aaatagtata taatatttat ataataaaca aactttaatt
     3841 ttaaaaaata agtctaatat acatagatat aattcataca attattataa ggatgttaat
     3901 gctttttaag tcttgacaag atcctactcc aaaatcagga aagaataatt gtatggcgaa
     3961 ttcaggtcaa tattatggat gagggggaaa aaacaatgct taaaacaatg tttaaaaggt
     4021 actctgtagt attaattaca tttatattac tgtttgtttt tcttacagga tgcgggtctt
     4081 caagcagtaa aagtgattct gcagttatgg aaacttctcg gggcggaggt gtttcttata
     4141 acagtgcttc taatagtaag gtaactctca cttcaaagca atcaaattat tatgatggcg
     4201 gtcaaagcgc tgcagatacc aactcaaata gtcaatctgc acaagatgcc aaagaggata
     4261 ttaaaaaggt aataaagaat ggggacatat ctgtcgaggt taaggatgta gatattgcat
     4321 atgaagggat aatgaagatt gtcgagagtt tgggcggtga agaattcaac aaaaattata
     4381 ctatctccgg tgaatacaag cgaatggaac ttgtccttaa aataccccct gagaaattga
     4441 atttatttga acaaaaaata aaggagtatg ttggagaggg aaaagtcagg aatattaaca
     4501 taagaagcag ggatataact tcggaatatt atgattatac tgcaaggctt gaaagctatc
     4561 aggcaagcag ggatcaaatg agggaacttc tgaaaaaagc tcagaatatt gatgaaaccc
     4621 ttagaataca acaagaattg acccgcctcc aagcagaaat agattctatg cagggtcagg
     4681 ttaaaatgtg ggataaactt atagatatgg caactattaa tttatatatt gatgaagagg
     4741 ataacccttt taaaaagaca actgctgtaa gctggaaatt taattcccct gctgaaatct
     4801 ggtcaactat gaagaatggt tttgtaggag ttgttaacac aatatacagt ttattgatat
     4861 ggatattaat tgctgtagta agcctttccc cggtcattgc agtggcaagc attgtaattt
     4921 atgtaatatt ctttgtgaaa aagaaaagga agcaaaaata gtccgcaaat tcttttgtgt
     4981 tgatataaaa agctaatagt atttttatac tgtatatgtt attattaata aacataaaat
     5041 ggatttaaaa aattaagtta tcatttaatg ggggctaagt tataggatgt ttaacgaaaa
     5101 gtttataaaa agtctgaaaa actttttaat atttttactg ctttatacgg ctgtgtttat
     5161 tcttgtttct aaaaccatgt cctatacctt cccttttata gcggcatttt gtattgcatt
     5221 tttaatgcag cctgtgacaa ggttttttaa agaaaaactt aagcttaata aaaatattcc
     5281 ttctctttta tcgtctgctt tagtattttt ggtacttttt gtattagtta ctttactgtt
     5341 ttataaaata atagtagagg caaagcagct ccttttgaat attccaaata tcaacatgga
     5401 acttataatg gaacccatac gtaaaattat caatgatata ggcttgtatt ttaaagacat
     5461 tgacccggat tttatagaga aaaacagcag acagatttcc gagataatat ctaatatttt
     5521 aagtgtattg ggaaagacac taaattcatt tttaactgta gccctgtcta tcccaatgtg
     5581 gatcactgtt atttttatag tcatactttc aacttacttt ttctcaagag atatggcggc
     5641 aattaaagct agagcagtat ccatattttc agagaaaggc agagacaagt tcatattggt
     5701 ctggtatgaa ggtgttaaaa tgctttctca ttatataaaa gcatacttgt ttatatacac
     5761 tttgactttt attcaaacat tagccggctt ttctatattg agaataaaat atgcagttat
     5821 tttgagtatt gtttgtgctg tagcagatgt tcttccggtc cttggtatag gaattatcta
     5881 cctgcctctt tcggcaatat attttatagc cgggaactat gttgcggcaa taggtataat
     5941 tattttgtac attattataa gcgtggtaag acagattgtt gaacctaaga tagtatccac
     6001 atcgctgggt attcatccgg taattgccct ggcaacaatt tttatcggat taaaagcata
     6061 tgggttttta ggtatgatat atttcacttt tttgatgata ttctataaaa tattaaaaac
     6121 tgcaaaggta ttgtaggcaa tggaaagctg gttagtactt tataaatttt ttgtgttaat
     6181 atattgctgt ataaaatata ttcagtatgg tatggaaaag gttccatata ttatactcgc
     6241 tgttcttata catatttcct taaatatgtt ctatcacatt ctcagaactt atagatgcag
     6301 atttattttt ttggtcgttt ctgctgtttt gttaataatt tccgggattt tcttgaatga
     6361 atattttcta atgcttttac catttaatat tttagaattg tggatgaatt tgtcggataa
     6421 catcaaattt agtgccgctg ttgctcttag tggagttatt tttgcaccaa tggggatgct
     6481 gccggaatat attcttttat atctgcttgg tacattatta tatataattt cttcagaata
     6541 tgataaaaca aaaatgaaga cttcagagga aaatgataac cttagagcag aattagatac
     6601 actttacatg cagattaata gaaacgagga ataccagagg cagatgatac atctctctca
     6661 attagaggaa agaaatttca tagcacagaa aatacatgac agtacgggac ataccattgc
     6721 aggagctctg atacagttgg aagcagcggc tcttcttatt gaaagggatg gaaagaaagc
     6781 acagggaatt attaaaaatg taacatcaat attgagagaa ggtatggaga acatcagaag
     6841 aacgttgagg aatattaaac caccttcaga acagcttgga atcagtaaaa tgaaaactgt
     6901 actaaatgaa ttttcactaa agcatgatgt aaaaactgaa ttctcgtata atggcaatat
     6961 tgacaaaata tcgtttatac aatggaagat aatttacgag aatcttatcg aagcgctcac
     7021 caatagcctt aaatattctc agtgtacaat tataaatgta acccttcagg tttttcacaa
     7081 agttgtaaaa tttgaggtaa aagataatgg acacggagca gcggctctga ataaaggtat
     7141 ggggttaaag ggaattgagg agagatgcgc aaatataggg ggaaaagtta taattgatgg
     7201 ctcaaaagga ttttctatta taactttact gccgatggag aattagttaa tatatatatt
     7261 gcagcgtata attatttgat cgcagttgac cgctgggata aaaataaatc ggaggtttga
     7321 aaatgtctgg aataccaata aaagtattga ttgcagatga tgatgccctt ataagagagg
     7381 gtttgaaaat cattcttgag ctggatgaca gatttcaggt tgtgggatgt gtagagaatg
     7441 gggagaaggc agtagaattc tgcatgaata atc
//
LOCUS       DAOSDD010000219         7426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3665_length_7426_cov_6.137702, whole genome shotgun sequence.
ACCESSION   DAOSDD010000219 DAOSDD010000000
VERSION     DAOSDD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3665_length_7426_cov_6.137702"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..888)
                     /gene="sigH"
                     /locus_tag="PK733_15595"
     CDS             complement(214..888)
                     /gene="sigH"
                     /locus_tag="PK733_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOJ11996.1"
                     /translation="MNSNAQLEIHSLYSVMDDEEIVIAAKAGNEKALNYLIYKYKNFV
                     RAKARTYFLIGADREDIIQEGMIGLYKAIRDYNEDKLSSFKAFAELCITRQIITAVKT
                     ATRQKHIPLNSYISLNKPIFDEESDRTLIDVIGGTETICDPEEMMISREEITGMETKL
                     NKVLSKLELKVLSLYLEGKSYQEVAVELNRHVKSIDNALQRVKRKLEKYYEEEKAKSG
                     VKIVMD"
     gene            1248..2216
                     /locus_tag="PK733_15600"
                     /pseudo
     CDS             1248..2216
                     /locus_tag="PK733_15600"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
     gene            2367..3104
                     /locus_tag="PK733_15605"
     CDS             2367..3104
                     /locus_tag="PK733_15605"
                     /inference="COORDINATES: protein motif:HMM:NF017328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HOJ11997.1"
                     /translation="MVPVLTILTFTSCWDYKDINNRTLPLSIGIDRIDDQVIYSGKIA
                     AITGGAAGKTSEKAEITSSYDFRAKGKDFEAAREDYDIAIAYPDFSGAIQVILFSKNY
                     AEVDGIESYIYRVNKLPEHRKTVLIAVSREPPDVILGTEIENDISPEFALENTIRYLH
                     DNGIRELERMISAKVKREIVEIVSKSQDLYKCDIFQFARYFNADNPHVFKKISWVEEY
                     PEAIVNIEVKTKNVSTNFLDLEAGEKH"
     gene            3124..3372
                     /locus_tag="PK733_15610"
     CDS             3124..3372
                     /locus_tag="PK733_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ11998.1"
                     /translation="MKIQLEAKAAQIAENNPDIIIRKISVMGKIVTILFLMQLTDREK
                     LSDAIIKPLVKYNGKEIGIVGTFLYKEAKLIKEEFVPT"
     gene            complement(3434..4252)
                     /locus_tag="PK733_15615"
     CDS             complement(3434..4252)
                     /locus_tag="PK733_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ11999.1"
                     /translation="MQNKQLRILTIGAHPDDCELTTGGICTKYKRQGHVVKYVYTTNG
                     NAGHHKMAGEKLAEVRAEEIRNACAIAGFEYEIMNNDDGYLESSVCNRDNLIRIIRKF
                     QPDIVFTHRPNDYHPDHRHTAILLQDASFLIRVPLICPDTPALSYSPVIMYMADSFKK
                     PMPFEPCVVVSIDDAIETKIKILDCHKSQFYEWLPWLDGYTEEIPASGRERIEWLTSS
                     QISRDNEIAERFRSELVKRYGKEKGLKIKCAEAFELSEYGRELKRDEIQAYFPF"
     gene            complement(4376..4753)
                     /locus_tag="PK733_15620"
     CDS             complement(4376..4753)
                     /locus_tag="PK733_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12000.1"
                     /translation="MVFGNMRKVVVIKNLPSDFIEEAIFILRVDGKTDNNLLKNDKAL
                     SGKSKTEGDCLIKEAQTIINNYIKECEKNGIITKTPNNTEINVINPQKSFTKKVPLNV
                     VINIALIGSIVAFIYLLVRILTF"
     gene            complement(4836..7373)
                     /locus_tag="PK733_15625"
     CDS             complement(4836..7373)
                     /locus_tag="PK733_15625"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ12001.1"
                     /translation="MKSEVSITGTRSYLNLISLKTYAIILPAAFLLGRVQLIKGLLPF
                     GLPMYIAAYELNVSRFAVAALILTGMATAGGGHNIFLTALGIILFSLLNAAFKAAFKD
                     KNSAKNRTASRDKMAAKDRFAGKEQAAAKNSLRSAVLGVISILIPNFVYVSIRGMLLY
                     ELLMAFFQAFIVFVIVFIIKKSFVLIDETRLGNSFTNEELISIAITGALVLSGFGDVK
                     IFGFGLKNVICIFTILMFSFRCGSAAGATVGVVAGLILSMSSTMAPLIIGCYAFCGLL
                     SGVFRNLGKVGTSLGFFMGNTVLTLYLNGSIEALIYLKEIILAVAIFMIIPQKFILKI
                     PESFGASPNTSMNEAIFSLRSKDVIVEKLNKFSRTFMEVSKTFNEISPVKAVVNKHDV
                     STMLDRVADRICKDCSLCLHCWDKNFYSTYQVMFKLVEKLEHKGFIEADDIPGYFISR
                     CERINDFITIVNNMYEIFKVDMMWKNRLGENRSLVSKQLEELSKAISGLAEEIEYDTG
                     FKTEVENTLVYMLKREGIKDAQVLAFENKHGKYEVSISYKGCEGEKKCTNIINKIVSD
                     VIGRKMIRDSFGCNKTISAYSNNININNININNTNSNNINSYNNCVLRFIEEETYSIT
                     TGVAYVSKYDERYESMVNGDNYTFINAKDGKFVTALSDGMGSGEKASFQSRTTISLIE
                     QFMETGFDKDITVKLINSILALNASDDYFSTIDLSVIDLNNGITEFVKIGAAPTYIKR
                     NDRVEVVKTASLPAGILGNMEVELVKKTLSDGDMLIMLSDGILDAFENSGQEKERTFL
                     KYVDEVRSINPQTVADKILDEAYKRSDSKPKDDMTVIVAKFWKKVSY"
BASE COUNT         2373 a         1395 c         1104 g         2554 t
ORIGIN      
        1 aatcctctct ttgtctacat tctaagggtt acgtcaatat atttcaaaag aacatcttac
       61 cactcagggt aatctttggg tcaagtgtaa aagatgcgcc caacaatttt ttgtgttttc
      121 aaattataac tcttcaaatc agaacactca atttataact tattattact taatcgtaaa
      181 aaattctaac tataattcca actattatgc cacttaatcc attacaatct ttaccccgga
      241 ttttgccttc tcttcctcat agtacttttc caatttgcgt tttacccttt gcagggcatt
      301 atctatagac ttgacatgcc tgttcagttc aactgcaact tcctgatagg atttaccttc
      361 aagatataac gataaaacct ttaattcaag tttgctcaat accttattaa gctttgtctc
      421 cattccggta atttcctccc ggcttatcat catttcctcg ggatcgcaaa ttgtctcagt
      481 tccacctatc acatcaatta gtgttcgatc agattcttca tcaaagatag gcttattcag
      541 agaaatataa gaatttagag gtatatgttt ctgccttgtg gcagttttaa cagcagtgat
      601 tatctgcctt gttatacata actcagcaaa agctttgaaa gaagaaagct tgtcctcatt
      661 ataatctctt attgctttat aaagacctat catgccttcc tgaataatat cttccctatc
      721 cgctccaata agaaaatatg tccttgcttt agcacgtacg aaattcttat atttataaat
      781 aagatagttt agggcttttt catttcctgc cttcgctgct ataacaattt cctcatcgtc
      841 cattacacta tataaagaat gtatttcaag ctgtgcattg gaattcaatt taacgccccc
      901 taatagtcac cactaattta aatattcatt gttgtggttg gttgacagtt atagctaaag
      961 cttttgtatc atcagaatac aagcatatta tatgtaataa ttgtaattat gtcaagtttg
     1021 catttttaac agttccattt ttaatatttt gattaatttc atttaatatg cacataataa
     1081 ctatagataa tgagcaaaag aaactacact ataaaagttt tgtacactta tgttacctta
     1141 actaatacac aaaaactaat acgcaaaaat ccaatttgtt ccttgtaaaa tcatttttgc
     1201 gttatatata catactttaa ccagcattta ctattggagg ctatattatg gatgaaagcc
     1261 tttcaagaag gcaaatagct tttgtattat tcggagcagc agcaggattt ggagtacagc
     1321 aactgcccaa agttttagca gagtatgtag gcacaggaac atggattgcc atattaatta
     1381 caaccacaat ggctattatt gctgtctata taaatacaaa gcttgcctgt gttcatgaag
     1441 gcatgactat ttatcattac agtaaaaccc ttgtgggcaa cccgggaaag tacatcatac
     1501 ttattatata tataccttat tatgttgctt tatctacatt tgtctccaga tatgccggag
     1561 aaattattaa agcacacctg cttataaaaa ctccggcctg ggctattgtg cttttatttt
     1621 taattttctc atactatgcg gtaagtaaga aactcaaggt aataagtatg ttatctgaat
     1681 tttttggagc tctgatattt ataacagtga tgtttttata atctgttgtt ttttcacagg
     1741 ggagtacatt atttttgcgg ccttaccttg gcgccggaaa tattgcatct tattttaagg
     1801 cttcttttaa aataattatt tcgtttcttg ggattgaagc gttaacaata atgcccataa
     1861 acaaaaaatc caagaataat cttttatttt atccaattgg aattatatta atacttggtt
     1921 ttttgtatat atgttccgtt gaatctgctt tatccgtcag aagtgtggat gatatagttc
     1981 attataaaga tacaataata gaaataatca aaagcgtcga cattccattc cttgaatttt
     2041 ttcgaagact tgatgctgtc atctttcttg cgtggattat ggcaattttg ggaaacatca
     2101 caattttagc ttacgcagct gtttaccttt tagccagctg gattgataaa ataaatttca
     2161 agttagttgc aggggtagtc tttctaatat catttattat ttcaattatt cccggagata
     2221 ctcagcaggt agagacaatt ctaaattata tcagctatgt tggtatcttt acagctttac
     2281 ttattccatt agcattacta attattacag aggtgaaaaa tcgtggtaag aataaaaaag
     2341 tatttaaata taaattattg caaatagtgg taccagtact taccatatta actttcacaa
     2401 gctgttggga ttacaaagat attaataatc gaacattacc tctttccata ggtattgacc
     2461 gtatagatga tcaagtcatt tattccggca aaattgcagc tataaccggc ggtgcagccg
     2521 gtaaaaccag tgaaaaggcc gagataacga gttcctatga ttttagagca aaagggaaag
     2581 actttgaagc tgcgagagaa gactatgata ttgcaattgc ataccctgat ttttcaggag
     2641 ctatacaggt tatattgttt agtaaaaact atgcagaggt agatgggata gaatcatata
     2701 tataccgggt taataagctc cctgagcata ggaaaacagt acttatagca gtaagccgcg
     2761 aacctcctga tgtaatcctg ggtacagaaa tagaaaatga tatatctccg gaatttgcat
     2821 tggagaacac aataaggtat ttgcatgata atggtataag ggagttggag cgaatgattt
     2881 ctgcaaaagt gaaaagggag attgtggaga ttgtgagtaa atctcaggat ttatacaaat
     2941 gtgatatatt tcaatttgcc agatatttca atgctgataa tccacatgta tttaaaaaaa
     3001 tcagctgggt agaggaatat ccggaagcca tagttaatat tgaagttaaa actaaaaatg
     3061 ttagcactaa ttttttggac cttgaagcag gagaaaaaca ttaaagaaca gggtgataat
     3121 acaatgaaaa tacaactgga agctaaagca gcacaaatag cagagaataa ccctgatatt
     3181 attatcagga aaatcagtgt tatgggaaaa attgtaacaa ttctcttttt aatgcagctc
     3241 acagacagag aaaaattatc agatgctatt ataaaacctt tagttaagta taatggtaaa
     3301 gaaataggta ttgtggggac attcctctat aaagaagcaa aactgattaa agaggagttt
     3361 gtccccacgt aatataaccg tgtcgccaag caatataaca atataatata gtttttttgt
     3421 gtgttatcta tagttagaaa ggaaaatatg cttgtatttc gtctcttttt aattcccttc
     3481 catattcact cagctcgaaa gcttcagcac actttatttt taaccctttt tcctttccat
     3541 aacgttttac aagttcagaa cgaaatcttt cagctatttc attatctctt gaaatctggc
     3601 ttgaagtcaa ccattcaatt ctttctcttc ccgaggcagg aatttcctca gtataaccat
     3661 caagccatgg cagccactca taaaactgtg atttatggca atccaggatt ttaattttgg
     3721 tttcgattgc atcatctata ctaacaacaa cacatggttc aaatggcatt ggctttttaa
     3781 agctatcagc catatacatt attacaggtg aatagctcaa agcaggggta tcaggacaaa
     3841 taagaggaac tcttataaga aatgatgcat cctgtaacag gatagcagta tgcctgtggt
     3901 caggatgata atcatttggt cggtgtgtaa atacaatatc cggctgaaac ttccttataa
     3961 tccttattag gttatcacgg ttgcatacac ttgactccaa ataaccatcg tcattgttca
     4021 ttatttcata ttcaaatcct gctattgcac aggcattcct tatctcttca gctcttactt
     4081 ccgccagttt ctcccccgcc attttatgat gtcctgcatt tccattggtt gtataaacgt
     4141 attttacaac atgcccctgc ctcttatatt tagtgcagat accacccgta gttaattcac
     4201 aatcatcagg gtgtgcacct atagtcaaaa ttctcaactg cttgttttgc attataatta
     4261 cctccaaatt aaacaataaa attcaatcag aaaaattcaa tgtctgaatg atatttgctt
     4321 tcaattgaat attttactgc ttctttgaaa gttttgcaaa catttttcga caaagctaaa
     4381 aagtcaaaat cctgactagc aggtatatga aagctacgat actcccgata agggcaatat
     4441 taataaccac atttaacgga acctttttcg taaatgattt ttgggggtta ataacattta
     4501 tctctgtatt atttggagtt ttagttatta ttccgttttt ttcacattcc tttatatagt
     4561 tgttaattat tgtttgggct tctttaatta ggcaatcacc ctcagttttt gatttgccag
     4621 ataatgcctt gtcatttttc aacaagttat tgtcggtctt tccatctacc cgtaaaataa
     4681 aaattgcttc ctctataaaa tctgaaggta aattttttat taccacaact tttctcatat
     4741 taccaaatac catagctttc ctccattaac ctgccagaat ccaaaaatat tcattagtat
     4801 aggtagtttg tgaaaatatt gtagtattta tacattcaat aacttacttt tttccaaaat
     4861 ttagccacaa taacagtcat atcatctttt ggtttactat cagacctttt gtacgcttca
     4921 tccaatattt tatcagcaac agtctgtgga tttatactac gtacctcatc tacatatttt
     4981 agaaaagttc tttccttttc ctggccgcta ttctcaaaag catcaagaat accatcgctt
     5041 aacataatca acatatctcc atcactcagg gtctttttca ccagttcaac ttccatatta
     5101 ccgagtatcc ctgcaggtag tgaagctgtt ttaacaactt caaccctatc gtttctcttt
     5161 atataggtag gtgctgcccc aattttaaca aactccgtta ttccattatt aagatcaatc
     5221 accgataaat caatagtaga aaagtaatcg tcgctggcat ttagcgccaa tatgctattt
     5281 attaacttaa cagttatatc tttatcgaaa ccggtttcca taaactgctc aatgagactt
     5341 attgtagttc ggctctgaaa agatgccttc tcacctgagc ccataccatc actcaaggct
     5401 gtaacaaatt tcccatcctt tgcatttata aatgtgtaat tatctccatt taccatactt
     5461 tcatatcttt catcatattt tgaaacatat gctacccctg tagttatgct atatgtttct
     5521 tcttcaataa accttagaac acaattatta taactattga tattattgct attagtatta
     5581 ttgatattta tattattaat atttatatta ttgctataag ctgaaattgt tttattgcat
     5641 ccaaagctat cccttatcat ttttctacca ataacatccg aaactatttt atttattata
     5701 tttgtacact ttttctcacc ttcgcaaccc ttataggaaa tactaacttc atatttacca
     5761 tgtttgtttt caaaagccag aacttgtgca tcttttatgc cctctctttt taacatatag
     5821 acaagtgtat tctctacttc agttttaaaa cccgtatcat attcaatttc ttctgcaagc
     5881 cccgatattg cttttgaaag ttcctcaagc tgctttgata caagactcct gttttctcca
     5941 agtctgtttt tccacatcat gtcaacctta aaaatttcat acatgttatt aacgattgta
     6001 ataaaatcat taattctttc gcatctgcta ataaaatatc caggtatatc atcggcttct
     6061 ataaatcctt tatgctcaag tttctccaca agcttaaaca ttacctgata tgtactgtaa
     6121 aagtttttgt cccaacagtg aaggcaaaga ctgcaatcct tgcatattct gtctgcaaca
     6181 cggtcaagca ttgtagagac atcatgctta ttaacaacag ccttcacagg agatatctca
     6241 ttaaaggttt ttgagacttc cataaatgtt cgagagaatt tgtttaattt ctcgacaatt
     6301 acatctttcg atcttaagct gaaaattgct tcattcatac tagtattagg agatgcgccg
     6361 aaagattcag ggatttttaa tataaacttt tgcggaatta tcataaatat agcaactgcc
     6421 agaattattt ccttcaagta tattagagcc tctatagagc cattaagata taatgtaaga
     6481 actgtgtttc ccataaagaa tccaaggctt gtccctacct tgccaagatt tctgaataca
     6541 ccggaaagca ggccgcaaaa ggcatagcat ccaattatta gaggagccat tgttgatgac
     6601 atgcttaaaa taagtcctgc tacaactcct acagttgcac ctgctgctga accacatctg
     6661 aaactgaaca tcagtatcgt aaaaatgcat attacatttt ttaaaccaaa tccgaatatt
     6721 ttcacatcac caaaccctga aagcactaat gctccagtta tagcaatact aataagttct
     6781 tcgtttgtaa acgaattgcc caacctggtt tcatcaatta atacaaatga tttttttata
     6841 atgaatacta ttacaaatac aataaaagcc tgaaaaaatg ccataagtaa ttcatacaaa
     6901 agcataccac gtatggaaac atagacaaaa ttcgggatca gtatactaat aactcctaat
     6961 accgcagacc gtaaactatt cttggcagca gcctgctctt tgccggcaaa tctgtcttta
     7021 gcagccattt tatctctgct ggctgtccta tttttagcag aatttttatc tttaaaagca
     7081 gctttgaaag cagcatttaa aagactgaaa agaattatcc ccaaagcagt caggaaaata
     7141 ttatgtccac caccagcagt agccattcct gttaaaatta aagcagctac tgcaaacctg
     7201 cttacattaa gctcataagc tgcgatatac ataggaagtc caaaaggcaa tagacctttt
     7261 atcaattgta ctctgccaag caaaaatgca gcaggtagta ttattgcata tgtttttaaa
     7321 gatataagat ttaaatatga tcttgtacca gtaattgata cttcagattt catcttttgt
     7381 cgacctcatt tctctcatta tctattgtta aaatttgcaa atattc
//
LOCUS       DAOSDD010000220         7419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3667_length_7419_cov_4.843427, whole genome shotgun sequence.
ACCESSION   DAOSDD010000220 DAOSDD010000000
VERSION     DAOSDD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_7419_cov_4.843427"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="PK733_15630"
     CDS             <1..1442
                     /locus_tag="PK733_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ12002.1"
                     /translation="PDMDGLELTGKIKDRYPYIHVIIISAYSDFEYAQQAISYGVSGY
                     LLKPLKDTELGKLLHKIKADLELIELMELSKINSSLNLNKTILNRISHDTILRRLLDG
                     DLIEGFDLSRFFEEREWSFIIKSVRVALCNIEFSESFTAGLFRKLAINTGLLCLGKMN
                     IPAIEHLGFIVIFLDESIRIQRREISKLLENYKHLFRMELEKLSTVKYNMTIGVSSLH
                     SNNVNLKKSFTEALYSYQHKYFIGNDNITFYEDLAFRNYSALSEDECSKIIDKIINII
                     FIGEENQLLDEVQNLFSYIEEQGDFTVEDINTKFIEIYFNISNAIKATNTTDIFTADG
                     DIINEIQTLTSYRHLKKWFNDRIINLYRMARRYSYDDSNWMVQKAMHYVKANISKKIT
                     LDDIAEYLHVNASYFSSTFKKNTGQNFIDYVTESKIKEAKHLLLNSTLKIKEISSKVG
                     YNDYSYFCKVFKSMENITPLETRMKGLIK"
     gene            1456..4215
                     /locus_tag="PK733_15635"
     CDS             1456..4215
                     /locus_tag="PK733_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ12003.1"
                     /translation="MIQKFHPKKISFKKYLVTVSMTVAFAILLSIGLIFYTRTIRYIN
                     DKLDLMEKNRFYSVYLDIDESIQSINRMIEYLQNSEILMNYLNIAENAKDDVYKKSVA
                     INDISKLLTNAKEYSAFTKRIFIITQAREYSNYSTTTFSFETIKRIRDYEFEDITFFC
                     YGMDRLHGIELRGISLLDENMFFAAGLRKNKGCDGKILAILDESFLYDTKQTTGNFYI
                     VDRSGDFIWPTIDNNDISNQTKKQISLNIQSHNMDEFMFKVNDKKVITKKFSNYNWFL
                     VYPVNDSIINQQKNSIIFYMFISFLSSIILTYIFSRLVSNRILKPIKKLTSVIGEYDG
                     KTDFTKYDIAESKGIYMRDNLILYFIISIVIPVIIFIVMFNVTSFKHIEAHTLGYYRT
                     VFDKTADRIDKYIERKLKAMQRITYSISIQQIAARRENAVLGVDEIFNIITENSVLGH
                     ERDIISIYNNTRELLFTNYYMGIPDFNNSFYEKMSDVKTGVIYDLQRDRYNRYLISIG
                     MPIFYQENSPIEGYIKQDIDNADILQLYLDLINDNREAYISDYNGIVISSPKIDIIGK
                     YSTSNAQNYNSSEGLINVKDRNSNKLLFYKKIRYLPWYLNYEVDYSIITGTSRELIMN
                     NIFIFIIVMLFVIIMAFIISNEIMKPLGKLNSYLFEFDPAKTGEKNKAEFFFIDEIST
                     LGSSFNKMSERIEELIDELIVSGMIQNRLEGEKKTAEIIALQAQINPHFLYNTLDMIN
                     YLVKQNKTDDVVMLINSLSDLFRFGISREEILIPIDEELKYTMAYTRIISVIYGDRIR
                     FIWNVDNNIRDYKTIRLILQPIVENAIYHGLKKREGRICITCSSYGEFIIFEVSDNGS
                     GMDNVELENIKENLEAGNSKKIGLYNVYSRLKLHFGEKVEFTIVSNLEEGTSVRISIP
                     KFESL"
     gene            4576..5532
                     /locus_tag="PK733_15640"
     CDS             4576..5532
                     /locus_tag="PK733_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12004.1"
                     /translation="MIQNIKIANIKSTDITISKTRYKEARKMWMLYLFLLFPIAHTII
                     FHYMPMYGVVIAFKDFKPVKGILGSEWNNFRHFKDLFTSTGFFRVVTNTLKISFLRLA
                     FGFPAPIVLALLLNEIWHVRFKKIVQTISYLPVFMSWIVLAGVFFEFLSVQRGPINYL
                     IKVLGGQPIDFFTDKRLFVIMLILTGIWQGVGWSAVIYLASLSSVNPELYESAELDGA
                     NRFHKAVHISLPSLTPVITILFILNLGGILDAGFDQIFNMSNPLVMEVADIIDTYVYR
                     MGLLNAEYDFSAAVGLFKNVVGLILIIITNTLVSRISDYGVW"
     gene            5627..6520
                     /locus_tag="PK733_15645"
     CDS             5627..6520
                     /locus_tag="PK733_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12005.1"
                     /translation="MKLKKKSIGGTTFDILNYTFMVLFSLSIIYPFWNLILLSFSDQR
                     EATNLGFRIWINNWDTTAYKYIFEHNNVAMGYFNTIFRAFTGTVLMLIMCYLGAYPLS
                     KKNLPGRNIITIFFLITMFFSGGLIPSYLLIKKINLMDKRLVYIIPGMFSTYNMIIMR
                     NFIMSMNKELEEAALIDGANYFDVAFKVIAPLLKPVLATIALWSIVGHWNSWFDCLIY
                     IKDEKKYVLQLIVRRIVIQTQGEAEFMKLQAFNQLSEKRIHSSNVKSAVILVTIGPIV
                     LIYPFLQKYFVKGIMVGSLKG"
     gene            6599..>7419
                     /locus_tag="PK733_15650"
     CDS             6599..>7419
                     /locus_tag="PK733_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12006.1"
                     /translation="MSKKVKRFLSLLIAIMLITTLFTACGKKENTGTQNDTGKGKTTA
                     GSDKEPKGFDDSKTLEISWFGFNGTEIVEGNPLQKEIEEKFNVKIKNIKADIFNKEQI
                     NLMINSGEMPDASFMYQTPIDLWDKGVTRAIPKSFIEKYSPNYKKLLDRYPIGWQLNK
                     VPDKEDEYLAINGINISLDENGWFSYYRLDWLENAGIKPKGELKQVDDEGRVFFCETP
                     FTLEEQDQIFDAFLKMNTDAKNPKYPASGNKDTTNYSWETILNSFGVKTWMNLEE"
BASE COUNT         2773 a          955 c         1309 g         2382 t
ORIGIN      
        1 tgccggatat ggatggtctg gaacttaccg gaaaaattaa agacaggtat ccgtatattc
       61 atgttattat tatcagtgca tatagtgatt ttgaatatgc acagcaagct ataagctatg
      121 gagtaagcgg atatcttcta aaacctttaa aagatactga acttggcaaa ctgcttcata
      181 aaataaaagc agatttggaa ttgatagaat taatggaatt atcaaaaatt aatagcagct
      241 taaatcttaa taaaactata ttaaatcgta ttagtcatga tactatattg cgaagattat
      301 tagatggaga tttgatagaa ggttttgact taagtcgatt ttttgaggaa cgagaatgga
      361 gtttcataat aaaatctgtt agggttgcat tatgtaacat agaatttagt gaatctttta
      421 cagccggtct tttcagaaaa ttggcaataa atacaggcct tctatgtctg ggaaaaatga
      481 atattccggc aatagagcat ttaggcttta tagtaatatt tcttgatgaa agtattagaa
      541 tacaaaggag agaaatttcc aagcttcttg aaaattacaa gcacctgttt agaatggaac
      601 tagaaaagtt gagcacagta aaatataata tgacaatagg tgtcagcagc ttgcacagca
      661 acaatgtaaa tcttaaaaag agctttactg aagccttata ttcctatcaa cataaatact
      721 ttattggaaa tgataatata accttttatg aggatctagc ttttagaaac tattctgcat
      781 tgagtgaaga tgagtgcagt aagataattg ataagataat caatattata tttatcggag
      841 aagaaaatca actgcttgat gaagttcaaa atttattttc atatatagaa gaacaagggg
      901 attttaccgt ggaagatatt aacacaaaat ttattgaaat ctatttcaat ataagtaatg
      961 caattaaagc aactaatact acagatattt ttactgcaga tggtgatatt ataaatgaga
     1021 tacaaacatt aacttcatat aggcatttaa aaaagtggtt caacgataga attattaatt
     1081 tatacagaat ggcaagacga tattcatatg acgattctaa ttggatggtt caaaaggcaa
     1141 tgcattatgt aaaagcgaat atatcaaaaa agattactct cgatgatata gcagagtatc
     1201 ttcatgtcaa tgcatcctat tttagttcta cttttaaaaa aaatacagga caaaatttta
     1261 tagattatgt aactgagtca aaaataaaag aggcaaaaca tttgcttctt aattcaacgt
     1321 tgaaaataaa agaaatatca agcaaggtag gatacaatga ctatagctat ttttgcaaag
     1381 tatttaaatc aatggaaaat attactccgc ttgagaccag aatgaagggt cttataaaat
     1441 aatggaggaa ttagtatgat acaaaagttt catcctaaaa aaatttcttt caaaaaatat
     1501 ttggttactg tatccatgac cgttgctttt gctatacttt tatcaattgg cttgatcttt
     1561 tatacccgta ctattagata tatcaatgat aagctggatc tcatggagaa aaataggttt
     1621 tacagtgtat atttggacat agatgaaagc atacagtcaa ttaatagaat gattgaatac
     1681 cttcaaaata gtgaaatatt aatgaactat ttaaatatcg ccgagaatgc aaaagatgat
     1741 gtttataaaa aaagtgttgc aataaacgat atttctaaac ttctaactaa cgctaaagaa
     1801 tatagcgcct tcacaaaaag aatatttatt attacacaag caagggaata cagcaattac
     1861 agtactacaa cattcagttt tgaaaccatt aaaagaatac gtgattatga atttgaagac
     1921 ataacatttt tttgttatgg aatggataga cttcatggaa tagaattaag agggatatct
     1981 cttctagatg aaaatatgtt ttttgcagcc ggtctcagaa aaaacaaagg atgtgatgga
     2041 aagatactgg ctattctaga tgaatcattt ctatacgata caaaacagac aaccggtaac
     2101 ttttatattg ttgatcggtc cggtgatttt atttggccta ctatagataa caatgatatt
     2161 agcaatcaaa ccaagaaaca aatttcccta aatattcaaa gccataatat ggatgaattt
     2221 atgttcaaag tgaatgataa gaaagttatt acaaaaaagt ttagtaacta taattggttt
     2281 cttgtttatc ctgttaatga tagtattatc aaccaacaaa aaaacagtat aatattctat
     2341 atgtttatat cgttcttgtc gagtataatt cttacatata ttttttcaag attagtatcg
     2401 aacagaatcc ttaagcccat aaaaaaactt acatcggtta taggcgaata tgatggcaaa
     2461 actgatttta ccaagtatga tattgctgag tcaaagggaa tctacatgag agataacctt
     2521 attttatact ttattatttc tatcgttata cctgttataa tatttattgt aatgtttaat
     2581 gtaacttcat tcaaacatat agaggctcac acattaggct attacaggac agtttttgat
     2641 aagactgcag atagaattga taaatatata gaaagaaaat taaaagctat gcagagaata
     2701 acttacagta tatccataca acaaattgcg gcaagaagag aaaatgcagt attaggagtt
     2761 gatgaaattt ttaatataat aacggaaaat tcggtgctag gacacgaaag agatattata
     2821 agcatttata ataatactag agaattgcta ttcacaaatt actacatggg gataccggac
     2881 tttaataaca gtttttacga aaaaatgagt gatgttaaaa ctggagtaat atatgaccta
     2941 caaagagata gatataatag atatcttata agtataggta tgcctatttt ctatcaagaa
     3001 aattcaccta ttgagggtta tataaagcag gatattgaca atgcagatat acttcaatta
     3061 tatcttgacc ttattaatga caatagagaa gcatatatat cagattataa cggaattgta
     3121 atatctagtc cgaaaataga tattataggc aaatattcga cttccaatgc ccaaaattac
     3181 aatagttctg aagggctaat caatgtaaaa gatagaaata gtaataaatt gttgttttat
     3241 aaaaaaatca gatacctacc gtggtattta aactatgaag ttgattacag tataattaca
     3301 ggtaccagca gagaattaat aatgaacaat attttcatat ttattattgt aatgttgttt
     3361 gttataataa tggcatttat tatatcaaat gaaattatga agcctctggg aaagctaaat
     3421 tcctacctat ttgagtttga ccctgccaaa accggagaaa aaaataaggc tgaattcttc
     3481 tttatagatg aaatcagcac cctgggcagc tcatttaata aaatgtcaga aaggattgag
     3541 gaacttatag atgaacttat tgtttcaggt atgatacaaa accggcttga aggtgagaaa
     3601 aagactgctg aaataattgc gttacaagcc cagataaacc ctcattttct ttacaatacg
     3661 ctggatatga ttaattatct tgtaaagcag aataaaactg atgatgtggt tatgctgata
     3721 aattctttaa gcgacttatt tagattcgga ataagtcgtg aagaaatatt aatacctata
     3781 gacgaagagt taaaatatac catggcatat accagaatta tcagtgtaat ttatggggat
     3841 aggataaggt ttatatggaa tgtggacaat aatataaggg attataagac tataagatta
     3901 atattgcagc caattgtaga aaatgcaata tatcatggat taaaaaagag agaaggtaga
     3961 atttgtataa cgtgcagtag ttacggagag ttcataattt ttgaagtttc tgataacggt
     4021 tcaggcatgg ataatgtgga gcttgaaaat ataaaagaaa atctggaagc cggtaatagt
     4081 aaaaagatag gattatataa tgtttattcc aggttgaaac ttcactttgg ggagaaggtc
     4141 gaatttacca tagttagcaa cttagaggaa ggtacttctg taagaatatc aataccaaaa
     4201 tttgaaagtt tataagtatc acataaatca tgctgatttt tactttctca agggattttc
     4261 tgatataaat acaactgtat ttatccaacc taactaacaa aaataatcca ataaaaatag
     4321 aaatatttat atttaataat actatttata atgatagtat tataaataga ttagtgcatt
     4381 actaaatgta attttatttc actgttgatc cggaaaacat cgcattccca attggcatag
     4441 cagcattagt ttattatatt gttaatattg agcaattaat ataaatttta tgctatttta
     4501 ggttaatata ataaaagtaa atttaccaga ctattagttg aactataaag taaaagtaaa
     4561 ggaagtaata ttgatatgat tcaaaacata aaaattgcca acataaaaag cacagatatt
     4621 acaatatcaa aaacaaggta taaagaagcc aggaagatgt ggatgctata tttgtttctg
     4681 ttatttccga ttgctcatac tataatattt cactacatgc ctatgtatgg tgtagtaata
     4741 gcttttaaag attttaaacc tgtaaaaggc atattgggca gtgaatggaa caattttcgg
     4801 cattttaagg atcttttcac atctacaggt ttttttagag tagttacaaa tacattgaaa
     4861 ataagttttt tacgattggc tttcggattt cctgcaccta ttgttcttgc tttattgtta
     4921 aatgaaatct ggcatgtaag atttaaaaag atagtacaaa caatatccta tttacctgtt
     4981 tttatgtcct ggatagtatt agccggtgta ttttttgaat ttctatcagt ccagagaggt
     5041 cctattaact acttaattaa ggttttaggg ggacagccta tagatttctt tactgataaa
     5101 cgactatttg ttataatgct gattttaacc ggaatatggc agggagtagg atggagtgca
     5161 gtaatatacc tggcatcttt atcatctgta aacccagagc tgtatgaatc ggcagaattg
     5221 gatggcgcca atagatttca caaggctgtc catatatcac ttccatcgtt gacacctgta
     5281 ataacaattc tatttattct taatttgggt ggaatactgg atgcaggttt tgatcagata
     5341 ttcaacatga gcaatcctct tgtgatggaa gtagcagata ttatagatac ctatgtatac
     5401 agaatgggat tgttaaatgc agaatatgat ttttcggcgg cagtagggtt gttcaagaat
     5461 gtagtaggct taatactaat aatcattact aacaccttag tcagccgtat atcggactat
     5521 ggtgtatggt gagaggatta tggcatatga tgaagcaatc gtagtatgtg ataattatca
     5581 tagtatgcag caattgatag aaattatctg aaaggaaggc agttaaatga aattaaagaa
     5641 aaaatccata ggtggtacta catttgatat attaaattat acttttatgg ttttgttttc
     5701 gttatctata atatatccct tttggaacct tattcttctt tcgttttctg atcagagaga
     5761 agcaacaaat cttggcttca gaatctggat taacaattgg gatactacgg catacaaata
     5821 catttttgaa cataataatg ttgcaatggg atattttaat acgatattca gagcttttac
     5881 aggtactgta ttaatgctta taatgtgtta tctgggggca tatcctcttt ctaaaaaaaa
     5941 tctccccgga agaaatatta taactatatt ctttttaatc acaatgtttt tcagcggagg
     6001 attaatacca tcatacctgt taataaagaa aataaacctt atggataaaa ggttggtgta
     6061 tataattccc ggaatgttca gcacctataa catgataata atgcgaaact ttataatgtc
     6121 tatgaataag gaattggaag aagcagctct gattgatggc gctaattact ttgatgtagc
     6181 atttaaggtc atagcccctc ttttaaaacc ggtattggca acaatagcgc tttggtcgat
     6241 agttggacat tggaattcat ggtttgattg tttgatatat attaaggatg aaaaaaaata
     6301 tgttcttcag cttattgtca gaaggatagt gattcagacc caaggtgaag ctgagttcat
     6361 gaaattgcag gcttttaatc aattaagtga aaaacggatt catagttcaa atgtaaaatc
     6421 agctgtcatt cttgttacca taggtccaat agtacttatt tatccattct tacagaagta
     6481 ttttgtaaaa ggtataatgg taggttcatt gaaagggtga taaagaaaat gtaaaagata
     6541 ttatgttttc tattatcaat ataataaaaa tataaaataa agcaaaggga ggattaatat
     6601 gagtaaaaaa gtaaaaagat ttctatcatt attgatagca ataatgctca ttacaacatt
     6661 gttcactgct tgtgggaaga aagagaatac gggaactcag aatgataccg gcaagggaaa
     6721 aacaactgca gggtcagata aggaacctaa gggttttgat gactcaaaga cactggaaat
     6781 atcttggttt ggttttaatg gaactgaaat tgtagaagga aatcctctac agaaggagat
     6841 tgaggagaaa tttaacgtaa aaattaaaaa tattaaagca gatattttta ataaggaaca
     6901 gattaacctc atgatcaact ctggagaaat gcctgatgca tcttttatgt atcaaactcc
     6961 tatagattta tgggataagg gtgtaaccag ggctatccct aagagcttta ttgaaaaata
     7021 ttctccaaac tataaaaaat tacttgacag atatccaatt ggatggcagc ttaacaaagt
     7081 accagacaaa gaagacgagt atttagcaat aaatggcata aatatttcgc tagatgaaaa
     7141 tggttggttc tcatactaca gactcgattg gttggaaaat gccggtatta aaccaaaagg
     7201 agagttaaaa caggtagatg atgaaggacg tgtgttcttt tgtgaaactc catttacttt
     7261 agaggaacaa gatcaaatat ttgatgcttt tttgaaaatg aataccgatg caaaaaatcc
     7321 taaatatcct gcttctggta ataaggacac aacaaactac agctgggaaa caatattaaa
     7381 ctcttttggg gtaaaaacat ggatgaatct agaagaaga
//
LOCUS       DAOSDD010000221         7410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3675_length_7410_cov_6.330116, whole genome shotgun sequence.
ACCESSION   DAOSDD010000221 DAOSDD010000000
VERSION     DAOSDD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3675_length_7410_cov_6.330116"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1752
                     /locus_tag="PK733_15655"
     CDS             241..1752
                     /locus_tag="PK733_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ12007.1"
                     /translation="MGNVKDSMKDSVDTSVNTYNVKGNMTKDTLLYLPAKAIEGIIGI
                     ATLYLFTRFFKPEEYGDYNLAVATVNITALFLFGWLFQAAYRYVNNFSGKRKISVFYS
                     TIFSVWTVISISTTVIGLILLLILRNRFDSYTVKFILFSILMFFTYSITQILFALLSA
                     ARIIKLNLILSILSAALKLALTTLYVKVLDAGIISTVVSIVLVDAGVIGIIVFRLKIY
                     RYISRHLFSRKVMKKFIQYGIPLVGVSLSLSLLSHSDRYIIKFFLGSEEVGIYTANYS
                     IASSVFSMLLLAIMRGVYPTILKTWKQNNISLTEELLSNAVRFFLIITVPAVVGISIL
                     SPVISRILDPLYISGSSVIIYVSIGMFFLGLTEYNNKAWELTSSTGVIFRNSLLCCLF
                     NIISNIILIPVAGYKAAAVNTALSYIFYFLLSFFGSRKILKWHLSTTNYLRIFGSAAL
                     MGLIIYTTTKVLSVNIPMLIVLVPAGMVVYGAGLYLTGEIKPEVRQLVAKVKK"
     gene            complement(1757..2830)
                     /locus_tag="PK733_15660"
     CDS             complement(1757..2830)
                     /locus_tag="PK733_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12008.1"
                     /translation="MHKSFFDMRAVYECGWNIFNRKGFSDDPKDISVNYNYLDHLSQA
                     GINWLVVFWTNANGFDKSWVDASKYAHSLGIKLARGFYGFCGGGPEYTMTEPNAPEHL
                     LRDTKWGPKMSVCPFEDEARQWMVSLLPKRLEPDIDGIFIEPAREISRNCICPRCQKL
                     LPYKWDVHVLNFLADEIHKIKPEAEVIPYLNMNESGEDKQKWAKELKALRKEIHHIFA
                     WGMDNDETLFDWLEAEPRFEHFAKLGRILLFPEGKKPQKSIEERVSSVFKWCRMAADR
                     GKKGYMFDYRVFGGREWKGHENDEPCTRISHRVPASLMLAGKAMQNPYLDEKEQSEFI
                     QELRGITDWDLDDQEYFYRGIDS"
     gene            2951..3133
                     /locus_tag="PK733_15665"
     CDS             2951..3133
                     /locus_tag="PK733_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12009.1"
                     /translation="MNKILVRSISESAVAGQGTLLSSNRYSFYVKEFPCILVACDYIN
                     SNLILIIQARRCKYED"
     gene            3123..3890
                     /locus_tag="PK733_15670"
     CDS             3123..3890
                     /locus_tag="PK733_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ12010.1"
                     /translation="MKIKNPYFKILPKVVEANKETTITILPLFEHVAFKQDSEYEILY
                     YPLDKPVQHDNSNESYGKKAVPENGTIKFTRVFEGEQEHILILNEINSRGKKIIDEFR
                     IYSLENDLYERYPYKGDLHMHSNRSDGVESPAYVAASCRKIGLDFMALTDHHRYYPSI
                     EAQEAFNDIDLDFKIFRGEEVHPPKNPLHTVNFGGSFSINELFENTEKYYSEIDELAD
                     KLGNIPDYVDKYEYVSSVWVCNKIREAGGLSIFCHPY"
     gene            3951..4433
                     /locus_tag="PK733_15675"
     CDS             3951..4433
                     /locus_tag="PK733_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12011.1"
                     /translation="MFDTQPYDAFELLGGYHLSEVESNTLQVARYHEEISKGKKIPIV
                     GSTDSHGCEKGSLFGWYYTIVFSHTSELQDIIGNIKSLHSVAVEALPNEKVRVYGPFR
                     LVKYALFLIREVFPAHDALCFEEGKLMLDYISGDKSVSDKLGRLKGQTTELMANFFGR
                     "
     gene            4587..4964
                     /locus_tag="PK733_15680"
     CDS             4587..4964
                     /locus_tag="PK733_15680"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOJ12012.1"
                     /translation="MIDYWVETANKDYQTMFNLFNSKDFHWSLFMGHLVVEKLLKAIY
                     VKNVNSNPPRIHDLLRLSEEAQVYTTEVQKDDLDLITTFNISARYPHFKHSFYKKCDY
                     EFTKVNIKKIEELRAWLLLTLEK"
     gene            4937..5257
                     /locus_tag="PK733_15685"
     CDS             4937..5257
                     /locus_tag="PK733_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ12013.1"
                     /translation="MATFNTREMIEKIARDFGTLVQKEFDVKNIYLYGSYAKGTNSED
                     SDIDIAVIGDDFSGDLIDDTFKLMRIRRKIDNRIEPRPFRTCDFNTSNPFSNEIMKTG
                     IMIM"
     gene            5488..5739
                     /locus_tag="PK733_15690"
     CDS             5488..5739
                     /locus_tag="PK733_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12014.1"
                     /translation="MFDTQPYDAFELLGGYHLSEVESNTLQVARYHEEISKGKKIPIV
                     GSTDSHGCEKGSLFGWHQSRWRNCLRLFKSSTEFVIEIQ"
     gene            complement(5844..6056)
                     /locus_tag="PK733_15695"
     CDS             complement(5844..6056)
                     /locus_tag="PK733_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ12015.1"
                     /translation="MNINENKKQIPLRLSQKLYEEIAAWAEADFRSINGQIEYLLSEC
                     VRKRKKVGKHNKKDPSVEDHGDSAGE"
     gene            complement(6072..7094)
                     /locus_tag="PK733_15700"
     CDS             complement(6072..7094)
                     /locus_tag="PK733_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOJ12016.1"
                     /translation="MEEKILKARPGMPMLILFVVLYFVSIGLIIIGGILLDKKYGIGG
                     VPLAIGITYTIFGFIPFLGLKIIKPQEALVLTLFGKYIGTLKESGFYYVNPFSVAVNP
                     ASKTRLGQSGDVDSAPASKLVIASGGNVTPAELANKKISLKIMTLNNVKQKVNDVLGN
                     PIEIGIAVMWRVIDTSKAVFNVDNYKEYLSLQCDSAVRNIVRIYPYDVAPGIDTTGDG
                     EPDEGSLRGSSAQVAERIRKGIQDKVKEAGLEIIEARITYLAYAPEIAAVMLQRQQAS
                     AIIDARKMIVDGAVGMVEMALEKLNQNSIVTLDEERKAAMVSNLLVVLCGNKDAQPIV
                     NSGSLY"
BASE COUNT         2335 a         1351 c         1341 g         2383 t
ORIGIN      
        1 ccagggtaag tataatatct gttacctggg gcttcgattc cagggggctt ctggagaagg
       61 gcaagcctga ttttattgct gctactcctg atgaaatggt aaatattatt tgcaatatgt
      121 cagattgcca aaaaaatatg tgaatatagc atatggtgct aagtataaag gggaatattt
      181 aatttataag aagaaaatat ttaataaatg tacccgtatg aaggtggatg tttgataata
      241 atgggaaacg taaaagatag catgaaagac agtgtagata cctctgtaaa tacatacaat
      301 gttaaaggca acatgacaaa ggatactcta ttataccttc ctgccaaagc aattgagggt
      361 attattggta ttgctaccct atatcttttc acaagatttt tcaaacctga ggaatatggt
      421 gactacaacc tggctgttgc tactgtaaat ataaccgccc ttttcctatt tggctggctt
      481 tttcaggctg catataggta tgttaataat ttttccggta aaagaaagat ttccgtattt
      541 tactcaacta tattttccgt ctggacagtt attagtatat ccacaactgt aattggactt
      601 attcttcttc tgattcttag aaatcggttt gatagctaca ctgttaaatt tatactattc
      661 agcatactta tgtttttcac atatagtatc actcagatcc ttttcgcatt attaagcgca
      721 gcacggataa ttaagttgaa cttaatactt tccatattat cagcagcgct taaattagca
      781 ctcacaacgc tttatgtgaa agttcttgac gccggtatta ttagtacagt tgtaagcatc
      841 gtacttgtag atgcaggagt tataggtatt atagttttca ggcttaaaat atacagatat
      901 ataagccgtc atcttttttc ccgtaaagta atgaaaaaat ttatacagta tggtattccg
      961 cttgtaggcg taagtctttc cttaagtctt ctcagccatt cagacagata cattattaaa
     1021 ttttttctag gctctgagga agtaggcatt tataccgcca actattcaat agcttcttca
     1081 gttttcagca tgttattgct ggctataatg agaggagtat atcctacaat tctcaaaaca
     1141 tggaagcaaa acaatatcag ccttaccgag gagctgctat caaatgctgt acgttttttc
     1201 ctaataataa ctgtccctgc tgttgtaggt atcagtattc tttcacctgt tatatccaga
     1261 attctcgatc ctctatatat tagtggcagc agtgttatta tttatgtttc catcgggatg
     1321 ttttttcttg gcctcactga gtataataat aaggcttggg aacttacatc cagtacagga
     1381 gtaattttta ggaacagttt gctgtgctgc cttttcaata taatttcaaa tattatatta
     1441 atacctgttg ccggctataa agcagcagcg gtaaacactg cactgtccta tattttttat
     1501 tttctgcttt cctttttcgg cagcagaaag atattgaaat ggcatctctc cacaactaat
     1561 tatttaagaa tatttggttc tgcagcttta atgggtttga ttatttatac tacaaccaaa
     1621 gtattatctg taaatattcc aatgctaatt gtactggtgc cggcaggtat ggttgtatat
     1681 ggtgcaggtc tttaccttac cggagaaatt aagcctgaag taaggcagct ggtggcaaag
     1741 gtaaaaaaat gagtcgttaa gagtcaattc ccctataaaa atactcctga tcatccaaat
     1801 cccaatcggt tatacctctc aattcctgaa taaactcact ttgctctttt tcgtccaagt
     1861 atgggttttg catggcttta cctgcaagca taagcgaagc cggaacccta tggctaatgc
     1921 gggtacaggg ttcatcattt tcatgtccct tccattccct gcctccaaat acccgataat
     1981 caaacatata accttttttt cctctatctg cagccatcct gcaccattta aacactgatg
     2041 aaaccctttc ctctatagat ttttgaggct ttttcccttc agggaaaagc agaattctac
     2101 ccagctttgc aaaatgctca aaacgtggtt ctgcttccag ccagtcaaac agagtctcat
     2161 cattatccat accccaggca aaaatgtgat gaatttcctt tctcagagct tttagttctt
     2221 ttgcccattt ctgcttatct tctccgcttt cattcatatt taagtatggt attacctcag
     2281 cttcaggttt aattttatgt atttcatctg ccaaaaagtt aagtacatgc acatcccatt
     2341 tatatggaag caatttctga catctgggac atatgcaatt cctggaaatt tctcttgccg
     2401 gttcgatgaa aatcccgtct atatccggtt ccagcctctt tggaagaagg ctgaccatcc
     2461 actgtcttgc ttcatcttca aaaggacata cactcatctt cgggccccat ttagtatctc
     2521 ttagcagatg ctcaggtgca tttggctctg tcatagtata ttcaggtccg cctccgcaaa
     2581 agccatagaa tcctcttgcc agtttaatcc ccaaagagtg tgcatatttt gaagcatcaa
     2641 cccatgactt atcaaatcca ttggcattgg tccagaagac cacaagccag tttatgccgg
     2701 cttggcttaa atgatcaaga taattgtagt ttacagaaat atcttttggg tcatcagaaa
     2761 atcctttacg gttgaaaata ttccagccac attcatatac agctctcata tcaaaaaaag
     2821 atttgtgcat gtatgttaat tcctccggta ctattgataa tattgataca acacatataa
     2881 tagtgatagt atatcaatat cgcagctaaa ctgcaaatat aagcaaaaat ggggggtgca
     2941 tataacttaa gtgaacaaaa tattggtaag gagcataagt gaatctgctg tagctgggca
     3001 gggaactctt ctctcaagca acagatattc cttttatgtt aaagagtttc cctgtatatt
     3061 agttgcatgc gattacataa atagcaattt aatattaata atccaagcga ggagatgtaa
     3121 atatgaagat taagaaccct tatttcaaga ttttacccaa ggttgttgaa gctaacaaag
     3181 aaacaaccat aactattctt cctttgtttg aacatgttgc atttaagcag gattcagagt
     3241 acgaaattct ttattaccct ctggataaac ctgttcaaca tgacaatagt aacgaaagtt
     3301 atggaaaaaa agccgtaccg gaaaatggga caatcaaatt taccagagtc tttgaaggtg
     3361 aacaggaaca catattgatt cttaatgaga ttaatagtcg aggtaaaaaa attattgatg
     3421 aatttagaat ttattcttta gaaaatgatc tgtatgaaag atacccttac aaaggtgatt
     3481 tacatatgca cagcaaccgt tcagatggag ttgaatctcc tgcatatgta gcagcttcat
     3541 gcagaaaaat cggcctggat tttatggctt tgactgatca tcaccgctat tacccttcaa
     3601 ttgaagccca agaggctttc aacgacatag atcttgactt taagatattc aggggagaag
     3661 aagtgcatcc tcccaaaaac ccattacata cagtaaattt tggtggaagt tttagtataa
     3721 acgagctttt tgagaatacc gaaaaatatt acagtgaaat agatgagtta gcagataagc
     3781 ttggtaatat ccctgattat gtggataaat atgagtatgt gtcatctgta tgggtttgta
     3841 acaaaattcg tgaagcaggc ggactttcta tattttgtca cccatattga atttacttgc
     3901 atgagaaatg caaacaggca tactatatct cctcaggatt aatatcacac atgtttgata
     3961 ctcagcccta tgatgcattt gagcttctgg gaggttatca tttaagtgaa gttgaatcaa
     4021 atacgctgca ggttgcccgc tatcatgaag aaatatcaaa aggcaaaaaa atacctattg
     4081 taggttcaac tgactcacat ggctgtgaaa aaggcagtct attcggctgg tattatacca
     4141 ttgttttttc gcatacttca gaattacagg acattatagg taatattaag agtctccata
     4201 gtgtagctgt tgaagcgctc cctaacgaga aagtcagggt ttatgggcct ttcagacttg
     4261 ttaagtatgc actctttcta atcagagaag tgtttccggc gcacgatgct ttatgctttg
     4321 aggagggcaa acttatgctt gattatatca gcggagacaa atcagtatca gataaattgg
     4381 gcaggttaaa agggcaaaca actgaactaa tggcaaattt ttttggtaga tgaggacaac
     4441 tatgacagta tgccaatata tgtccttgat agagcaaatt aatcacataa tataaatgag
     4501 tgtattgtgc tcaaaaggat tgtaggttat aatatgattg tacaattaca ttgtcttgag
     4561 aaggtgagaa tgtggaaaaa taagctttga tagactactg ggttgaaact gcaaataagg
     4621 actatcagac tatgttcaat ttattcaata gcaaagattt tcactggagt ttatttatgg
     4681 gacatttggt tgtagaaaag ctgcttaaag ctatttatgt taagaatgta aattccaatc
     4741 cacctagaat acacgattta ctaaggttat ctgaagaagc gcaggtttat acaactgagg
     4801 tgcaaaaaga tgatttagac ttaataacaa cttttaatat aagtgctaga tatccacact
     4861 tcaaacatag cttttataag aagtgcgatt atgaatttac caaggttaat ataaagaaga
     4921 ttgaggagtt gagagcatgg ctacttttaa cactagagaa atgatagaaa aaattgcaag
     4981 ggattttggt acgctagtgc aaaaggaatt tgacgtaaag aacatatact tatatggttc
     5041 atatgcaaaa gggactaatt cagaagacag tgatatagat atcgctgtaa ttggggatga
     5101 tttttcaggt gatttgatag atgatacatt taagttgatg agaataagga gaaagattga
     5161 taatcgaatt gaacctagac cattcagaac atgtgatttt aatacatcta atccattttc
     5221 taacgagatt atgaagactg gaataatgat aatgtaaacg agctttttga gaatatcgaa
     5281 aaatattaca gtgaaataga tgaattaaca ggtaagcttg gtaatatccc tgattatgtg
     5341 gatatatatg aatatgtgtc atctgtatgg gtttgtaaca aaattcgtga agcaggcgga
     5401 ctttctatat tttgtcaccc atattgaatt tacttgcatg agaaatgcaa acaggcatac
     5461 tatatctcct caggattaat atcacacatg tttgatactc agccctatga tgcatttgag
     5521 cttctgggag gttatcattt aagtgaagtt gaatcaaata cgctgcaggt tgcccgctat
     5581 catgaagaaa tatcaaaagg caaaaaaata cctattgtag gttcaactga ctcacatggc
     5641 tgtgaaaaag gcagtctatt cggctggcac cagagcagat ggaggaattg tttgcgacta
     5701 tttaaaagct caacagaatt cgtaattgaa atacaatgag gtttgcaacc tatattatta
     5761 ttcaggaaga tagcaataaa accagcaatg ctatcttccc agattaaacc ataaactggt
     5821 cactaaccag ttttttattg ttattactca cctgcactat ctccatggtc ttctacagaa
     5881 ggatcttttt tattatgttt ccctaccttt ttcctttttc taacacactc agaaagaaga
     5941 tattcgatct gtccattgat agaccggaaa tcagcttccg cccaggccgc aatctcttca
     6001 tacaattttt gtgataaccg aagaggtatt tgtttcttgt tttcatttat attcatgact
     6061 aatacccatt cttaataaag actaccacta tttacaatag gttgggcatc tttgttacca
     6121 cataaaacga ctaataaatt agacaccata gcagctttac gttcttcatc aagagttaca
     6181 atcgaatttt gattgagttt ttccaaggcc atttccacca taccaacagc tccatcaaca
     6241 atcatttttc ttgcatcaat aatagcactg gcttgctgtc tttgtaacat aacagcggca
     6301 atttccgggg cataagcgag ataggtgatc ctagcttcta taatttcaag acctgcttct
     6361 ttaactttat cctgaattcc tttgcgtatc cgttccgcta cttgtgcgct tgagccccta
     6421 aggctgccct catctggctc accatctccg gtagtatcaa tgcccggggc aacatcgtag
     6481 ggatatatcc gtacaatatt tcttacagca ctatcacatt gcaatgataa atattcttta
     6541 tagttatcaa cattaaatac agcttttgaa gtgtctatta ctctccacat aacagcaatc
     6601 cctatttcta taggatttcc aagtacatcg tttacctttt gcttgacatt attcaacgtc
     6661 ataattttta aagatatttt cttgtttgcc aattcagccg gagttacgtt tcccccggat
     6721 gcaatgacca atttagatgc cggagcactg tccacatccc cactttgacc tagacgagtt
     6781 ttagaagcgg gatttacagc tactgaaaac ggattgacat aataaaagcc ggactctttc
     6841 agggtaccga tatatttccc gaatagagtt aaaaccaagg cttcttgagg tttaatgatt
     6901 ttaagaccga gaaatggaat aaagccaaat atagtataag tgatgccgat agctaaagga
     6961 actcccccta taccatattt tttgtctaaa agaatcccac ctataataat aagaccaatt
     7021 gatacaaaat aaagcacgac aaatagaatt agcatcggca tacctggtct tgctttcaaa
     7081 attttttctt ccatgattac cacctcttat atgtatttat gatattgtta tgatatcatt
     7141 atcatatcat tataatatta taataccatg gctgacaaaa ttgtcaagca ataaaatatc
     7201 ttttgggtgc atttaattta tctgaagaaa atattggtaa ggggtataag tggaggtgct
     7261 gtagtttggc aggggaaact cttctctcaa gtatcaggca ttccttttat gctagagagt
     7321 ttccccatat ctgagcgaag tgatagcaaa atttaaaggc tctaagcacc gtaacgatag
     7381 cccctggtaa atatttcttc agatttgata
//
LOCUS       DAOSDD010000222         7406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3678_length_7406_cov_4.648619, whole genome shotgun sequence.
ACCESSION   DAOSDD010000222 DAOSDD010000000
VERSION     DAOSDD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3678_length_7406_cov_4.648619"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PK733_15705"
     CDS             complement(<1..1136)
                     /locus_tag="PK733_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12017.1"
                     /translation="MGIMTVLNTIEKDISKQEVKTESYFRVEGQKYFLGNRYIERVWK
                     YDKGSLYTVSILDKVAGYQWVLKHSTIHNSEKDNDWLREISYEGLTNDIRQKDSEERS
                     EYEMQLVDVSSNKVNDSIYSGSYLEIVFHLRDKVHCMDVKRHAVVYDNAPAIRTFIEV
                     KSDKMPMGDFFSGTRLNILDTYPVALDNKYLRSYEFFARTDLTNHLVEERLNPSGFDK
                     GSLVFYCPPDSEGLFFHKESPCFSDQRPEVEGNFRICEKRIETLGWGIRPEEIPEEYK
                     ITYSSVIGVFRGNYNCALLALKEYQRERSLIIPERDYSVMANLWGDRQCVEHMNEEFV
                     IKEMEACAKLGVTHYQLDFGWQKRGDKDYWDVHPERFPGGFGRI"
     gene            complement(1531..3792)
                     /locus_tag="PK733_15710"
     CDS             complement(1531..3792)
                     /locus_tag="PK733_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12018.1"
                     /translation="MNRITRIFHSKSIYAQLLAGFLTVILIVSSFQLILNVVYKNNIE
                     RELIDKTHKRLNYVALGIEQQFENIITNVLISLYQKYGEQLRTRTMSAVDIERAKATI
                     LDYCNKNSFIENIIIIDLKGNIVIDCNSACNKDMYLSKILSGSDDYWDLFIKQLEEDF
                     TFKILPVHNFTEYSSFDWVGNNTRPLIPVIYKEKTGSSFTITALVNPKSLVKIIEPDF
                     MNDFYIYNLEGQAIFPLEILEDKNIKDIKYNLGSKEGLTRNNEGFISIKRFSFTSLVF
                     CKYIPDETFRIYTRQTKFLLNIAIIISVLLGSIASVFIIKGFNNSVKQIVEMIGKAKN
                     ISGDKSGTINLKYIANNVRNIVEENMRYENDIDKKDSILKSVFYREKLLNVISQEKEM
                     PYGFDGKSEIFSQYIIIIFKVCCREKFYSDIKLEYSKVIFIINELIERYLKQLFGQAI
                     SFQVENDNITSIVSAENNKNIEEKINVLVDKLNAENKYVFFTIIVSKTYDDFSQLNNV
                     YKRINSLEKNRNLCNETQIMTEGNCEIEDSIYFTNTQREQIVNLLSNKQIDECIKHVN
                     DILKYNADKKVSSFSIFLLCADIVNSCNDALSRLYSRIPPDFNMKNIYSMIKMYETIE
                     NYQISCENIIITYYNYICTHTPSEDDIVRNVKEYVNQHYMEDISIDMLAEKFCISRSY
                     LSRHFRNETGECLSDYINQIRMQEASRLLKNTSLKINEIYLKVGITNNVTFNRLFKKY
                     TGMTPNIYRRAER"
     gene            complement(4097..5509)
                     /locus_tag="PK733_15715"
     CDS             complement(4097..5509)
                     /locus_tag="PK733_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOJ12019.1"
                     /translation="MWIQFSIMALYFMIVVVVGIAAKRKSVSLSTYHGENLGFFVAVA
                     ASTGEWLGGTSTIGISEYGYSYGISGAWYTVANGIGLLVLGIFFAKLYRSMDKVTIPG
                     IIGNVLGEKARMVSSVFLIFVMLVVGTAQIIAAGSFGVLVLKLDFNLSVILLGAGFVI
                     YTLIGGMSAVGYTSVLHFIIKYGGIILALVVSIKGIGSCKTIQSSLPPEYFNWLNIGL
                     PRVSSWIIASVLGTCTAQAGIQPILAARDEITAKKASIAGALIVAPFGILTALLGMIA
                     RAKFPHITDAKLALPTLMINMNPVAGGIVLASVLAAILSTSSPIILACGTMFTKDIYQ
                     PRRGSKASESKLLVVSRISTAISGVICIALAIAINGSTKILDIVYFAYTIRGALFIVI
                     LAGLFWKKVSQKGAIISMFSSVITGSIWVVYKSIFGNFPIHPAITETYASVIVAIATV
                     LVFSVLFPDKSYSIKNKAIT"
     gene            complement(5531..5857)
                     /locus_tag="PK733_15720"
     CDS             complement(5531..5857)
                     /locus_tag="PK733_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOJ12020.1"
                     /translation="MKTVKLTEIAKTIRSKNAGVNKITFDIIFTDKDKYEMVKASKVL
                     SKSTIAKLYDIPEERICDFVEFDPAYAIKFTIMRSKPSGSVGEGDVFGCQQYPPLLEL
                     EIPVAE"
     gene            complement(5993..7357)
                     /locus_tag="PK733_15725"
     CDS             complement(5993..7357)
                     /locus_tag="PK733_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOJ12021.1"
                     /translation="MKKELRIICPNGHLGFAPTKEESFYIGAKTHPDYYCSDSGSDDI
                     GPTPLGEDICASHYNWQKHDLELMLVAAREQGVPMIIGSAGDTGTNSRVDMYIDIIRD
                     LAKKHNIPKFKLAYFYSNVDKQYLYDKLKKGVQVKGLEGYSDLTPEVLDKTNRIVAVA
                     GVHPYIKALEGGADVIIGGRSSDIAIFAAPAIKEGFPENLSYYLGKVLECASFCAEPY
                     GAKESVIGTITKDDVKVTAMHPKQRCTIASVAGHAMYERSNPYYEYFAGGMIDMSQCK
                     YEQFDEKTCRITNPKYIPTEGKITVKLEGSGKIGERYIGIAGIRDPYSIQNIDKVLDW
                     ARSQVKERFPNEEYHLSYRVFGKNGVMGDLEPVKEIKSHELCIVVEGVAPTDEVAHEV
                     TMIGLRQLFYARLPEVKGTAGSAAFLLDEVIKASPAYEWTMNHIVPVDDPMELFDLKF
                     IDIG"
BASE COUNT         2225 a         1528 c         1043 g         2610 t
ORIGIN      
        1 attatcctgc cgaaaccacc gggaaaacgc tcagggtgga catcccaata atctttatct
       61 cctctcttct gccatccaaa atccagctga tagtgtgtaa ctcccagctt tgcacaagct
      121 tccatttcct taatgacaaa ctcttcattc atatgttcca cacactgtct atctccccaa
      181 agattggcca taaccgaata atctcgttca ggtataatta atgacctttc tctttgatat
      241 tctttcagag ccagcagtgc acaattgtaa tttcctctaa acactcctat tacagagcta
      301 taggtaatct tatactcttc aggtatttcc tcgggacgta taccccaccc taatgtttct
      361 attctttttt cgcatatacg gaagttgcct tccacttccg ggcgctgatc agaaaagcat
      421 ggactttctt tatggaaaaa taatccttca ctatccggcg ggcagtaaaa tacaagactg
      481 cccttatcaa agcctgaggg attaagtctt tcttctacaa gatggttagt taaatccgtc
      541 cttgcaaaaa actcatatga ccgcaaatat ttgttatcaa gtgccactgg gtaagtatca
      601 agtatattga gccttgtccc gctgaaaaaa tcacccatcg gcattttgtc tgacttcact
      661 tctataaatg ttcttatagc cggagcatta tcatacacaa ccgcatgccg ttttacatcc
      721 atacagtgca ctttatccct gaggtgaaaa acaatctcaa ggtaagagcc cgaatatata
      781 gaatcattaa ccttatttga actaacatct acaagctgca tttcatactc tgatctctct
      841 tcggaatctt tttgccttat atcgttagta agaccttcat atgatatctc cctgagccaa
      901 tcattatcct tctctgagtt atgtattgtg ctatgcttta atacccactg gtatcctgca
      961 accttatcca atattgaaac tgtatatagg gaacctttat catacttcca aactctttca
     1021 atgtaacggt ttcccagaaa gtatttctgt ccttcaactc tgaaatagga ttccgtcttt
     1081 acttcttgtt tagatatgtc tttctcaata gtatttaaca ctgtcattat ccccatattt
     1141 tgccctccgt cagtccaaat tgtagtgtta aaatcttctc attataatct cattaagaat
     1201 aataataacg actgcagcta catctatatt atatatgaaa aatacagtat gcttatatgc
     1261 cgcatctatg ctatactctg cattttaaat tttataatta atgcttattg gagtaaagac
     1321 acaatttgtt acattataaa catccagttg tcaaggttca taaaatgtta tatataaaac
     1381 aaaatgttat ttttttaata ttatctaaaa gagaatacaa aacagcccta cctgaaaatg
     1441 gtttatggca gggctgaaag atccaaatat gtttctattt tattatatat aacgtaaaat
     1501 gattctacac ggtttataaa ctcagcagac ttatctttcc gctcttctat agatattagg
     1561 tgtcatacct gtatattttt taaacaatct gttgaaggtt acattattgg ttataccaac
     1621 cttcaaatat atttcattta tctttaaaga agtatttttc aaaagtcttg atgcttcctg
     1681 cattcgtatt tgatttatat aatcactcaa acattcacct gtttcatttc taaaatgtct
     1741 cgaaagataa ctccgggaaa tacagaattt ttctgccagc atatctatac tgatatcttc
     1801 catatagtgc tggtttacat actctttaac attacggaca atatcatcct ccgacggtgt
     1861 gtgagtacag atatagttat aatatgttat aattatattc tcacatgaga tttgataatt
     1921 ttctatagtt tcatacattt taatcattga atatatgttt ttcatgttaa aatcaggcgg
     1981 tatcctgcta tataaccttg acaaagcatc attacagctg ttaacaatat cagcacacag
     2041 caaaaatatt gaaaaactgc ttactttctt atcagcatta tattttaata tgtcgttaac
     2101 atgttttatg cattcatcaa tctgcttgtt tgaaagtaag ttcacaattt gttcacgctg
     2161 cgtgtttgta aaataaatag aatcttctat ctcacaattt ccttcagtca ttatctgagt
     2221 ttcattacac agattcctgt ttttttcaag gctattgatt cttttgtaaa cattgttcag
     2281 ttgtgaaaaa tcatcatatg ttttgcttac gataattgta aagaaaacat atttgttttc
     2341 agcattcagc ttatctacca gcacatttat tttttcttca atatttttat tgttttcagc
     2401 actaacaatt gatgtgatat tatcattttc aacctgaaat gatattgcct gcccgaataa
     2461 ttgtttgaga tacctttcga ttagttcatt tattataaat ataaccttgc tatattccag
     2521 ttttatatca gagtaaaatt tttctctgca gcacaccttg aaaataataa tgatatattg
     2581 ggaaaatatt tcacttttac cgtcaaatcc atatggcatt tctttttctt gtgatatcac
     2641 atttaaaagc ttttccctat aaaaaaccga tttcaatata gagtcctttt tatcaatatc
     2701 attttcatat cgcatgtttt cttcaacaat atttcttaca ttgttagcta tgtatttcaa
     2761 attaatagtg ccacttttat ccccagatat atttttagct ttacctatca tctcaacaat
     2821 ctgctttaca gaattattaa agcctttaat aataaaaact gaagcaatac ttcccaataa
     2881 tacagatata ataattgcta tattaagcag aaattttgtt tgccttgtat atattctaaa
     2941 tgtttcatca ggaatatact tgcagaatac caaagatgta aaagaaaacc tctttataga
     3001 tataaatcct tcattgtttc ttgtaagccc ttccttgctt cccaagttgt attttatatc
     3061 tttgatattt ttatcttcta gaatttctaa aggaaatatt gcctgccctt ccaaattata
     3121 aatataaaaa tcattcataa aatccggctc aattattttt acaagagatt ttggattaac
     3181 caatgcagtt attgtaaaac tactccctgt tttttccttg taaatcaccg gtataagtgg
     3241 tcttgtatta tttccgaccc agtcaaagct tgaatactca gtgaagttgt gaacaggaag
     3301 aatcttgaat gtgaaatcct cctccagttg ttttatgaat agatcccaat agtcatctga
     3361 acctgacaag attttgctaa gatacatgtc tttattgcat gcactgttgc aatctataac
     3421 aatattacct tttaagtcaa ttataattat gttttcaatg aaactatttt tattacagta
     3481 atcaagtatt gttgctttcg ctctttctat atctactgct gacatggttc ttgttctcaa
     3541 ttgttcacca tatttttggt ataagcttat cagaacattt gtgataatgt tttcaaattg
     3601 ctgttctatc cctaaagcaa cgtaatttaa tcttttatgc gttttatcta ttagttctct
     3661 ttctatatta tttttataaa ctacgtttaa tattaattga aatgaagata caatcagtat
     3721 tacagtcaaa aaacctgcca ggagttgagc atatattgac tttgaatgaa aaatacgcgt
     3781 aattctattc ataataattc atagcacccc tgtcaataaa ttaattatac caaatataca
     3841 atatatctat aacacatcat aatatatcat atcttagcac atagttgcaa tccagtatat
     3901 gcatgcgggc acattttctt cctaaattat tagggacgtt tctacatttc tacagaatta
     3961 ttattattgg ggacgtttct acagctatat aaaaagagcc tgacataatg aattaataat
     4021 tcaattaatc aagctctttt ctattttata tttgctatat aaaacaagat aactgttttg
     4081 tattacaatc tttttattaa gtaattgctt tgttctttat agagtagctc ttatccggaa
     4141 acaaaacact aaatactaat acagttgcga ttgccactat aacggatgca taagtttccg
     4201 taattgccgg atgtattggg aaattaccaa atatactttt gtaaactacc catatggagc
     4261 ctgtaattac agatgaaaac atcgagataa ttgccccttt ctgggaaacc ttcttccaga
     4321 ataatccagc cagtataact atgaataatg ctcctcttat tgtatatgca aagtaaacta
     4381 tatcaaggat cttcgtgctt ccattaattg ctatcgccaa tgctatacat attacaccgg
     4441 atattgctgt agaaattctg gataccacaa gcagtttact ctcagaagct ttagaacccc
     4501 gccttggctg ataaatatct ttggtaaaca tggtaccgca tgccagaata ataggtgaag
     4561 atgtagataa tatagcagcc agaactgatg ctaaaacaat accccctgca acagggttca
     4621 tattaatcat taatgttggc agagcaagct ttgcatctgt tatatgagga aacttcgctc
     4681 gtgctatcat tccaagcaat gctgtaagaa taccaaacgg ggctacaatc aaggctcctg
     4741 caattgaagc tttttttgcg gtgatctcat ctcttgctgc aaggataggc tgtattcccg
     4801 cttgggctgt acatgtgccc agaacagatg caattatcca ggatgaaact cttggtaatc
     4861 ctatattgag ccagttgaaa tactccggcg gcagacttga ttgaatagtt ttgcagctgc
     4921 cgataccctt aattgaaact acaagtgcaa gtataattcc cccatatttt atgataaaat
     4981 gaaggacact ggtataccct actgcactca tacctccaat cagagtatat ataacaaatc
     5041 ctgctcccag aagtattacc gacaaattaa agtccagctt taatactaga actccaaaag
     5101 aaccggcagc tattatctgt gctgttccaa ctaccagcat aacaaatatc aggaaaacac
     5161 ttgataccat tctggccttt tcacctaaaa catttcctat aataccaggt atggttactt
     5221 tatccatgct tctatacaac ttggcgaaaa aaatgcctaa aaccagaagt cctattccgt
     5281 tagctacagt ataccaggct cctgaaatcc catagctgta accatattcc gatatcccta
     5341 tagttgatgt cccacccagc cattcccctg ttgatgctgc gacagcgaca aaaaagccca
     5401 gattttcacc atgatatgtg cttaaagaaa ccgacttcct tttcgccgct atgccaacaa
     5461 caacaacaat catgaaatat aatgccatta ttgaaaattg tatccacatt gttattattc
     5521 tccataccgc ttattcagca acaggtattt caagctccag taaaggcgga tactgctggc
     5581 acccaaaaac atcgccttcc ccaacacttc cacttggttt tgatctcata atagtaaatt
     5641 taattgcata agcaggatca aactctacaa aatcgcaaat tctttcctcc ggtatatcat
     5701 acagctttgc tatagtgctt tttgataaaa ccttgcttgc ttttaccatt tcgtatttat
     5761 ctttatctgt aaatattata tcaaaggtta ttttattaac ccctgcattt ttacttctta
     5821 tagttttagc aatttcagtt aacttaactg ttttcaattt acttgcctcc ctttacattc
     5881 ttttcattct ttttgatgat attccttttt tatggagaac tgtccccacc tttgtcccca
     5941 cttttgatgg agaactgtcc ctgctttttc cctactttag cttgtcttgt atttatccaa
     6001 tatcaatgaa tttcagatca aacaactcca tagggtcatc tacaggtaca atatgattca
     6061 ttgtccactc ataggcgggg ctggctttaa taacttcatc aagaaggaat gctgctgatc
     6121 cagctgttcc ctttacttcc ggcagtcttg catagaaaag ttgtcttaat ccgatcattg
     6181 taacttcgtg ggctacttca tctgtaggtg ctacaccttc gactacaata caaagctcat
     6241 gggacttgat ttcctttaca ggctcaagat cacccattac accgttcttg ccaaacactc
     6301 tgtaacttaa gtgatattcc tcatttggga aacgttcctt aacctgtgat cttgcccaat
     6361 ccagtacctt atcaatgttt tggatggaat acggatcacg gattcctgca attcctatat
     6421 aacgttctcc tattttccct gatccctcaa gtttgaccgt gattttccct tctgttggta
     6481 tgtatttagg attggttatt ctgcaagttt tttcatcaaa ctgttcatat ttgcactgac
     6541 tcatatcaat cattccgccg gcaaaatatt cataatatgg atttgatctc tcatacatgg
     6601 catggcctgc gactgatgca atagtacatc tttgctttgg atgcattgca gttactttca
     6661 catcatcttt tgtaatagtc ccaataactg attcttttgc tccatagggt tctgcacaga
     6721 atgatgcgca ttcaagaact tttccaagat aatatgataa gttttctggg aatccttctt
     6781 taattgctgg tgcggcaaat attgcaatat cacttgacct tccacctata attacatcag
     6841 caccaccttc aagagcctta atatacgggt gaacaccggc tactgcaaca attctattgg
     6901 ttttatccaa tacttccggt gtcaaatcac tatatccctc aaggcctttt acctggacgc
     6961 cttttttcag tttatcatac aaatactgct tatctacatt agaatagaag tatgcaagtt
     7021 taaatttggg tatattatgc tttttagcca gatcccttat tatgtcaata tacatatcta
     7081 ctctgctgtt tgtaccggta tcccctgctg acccgataat catgggaaca ccctgctctc
     7141 ttgctgcaac aagcatcaat tccagatcat gtttctgcca attataatgg cttgcacaaa
     7201 tatcttcacc aagcggtgta ggtcctatat catcactgcc tgaatcgctg cagtaataat
     7261 caggatgtgt tttagctcct atataaaagc tctcttcctt tgtaggcgca aatccaagat
     7321 gtccgttagg acagataatt ctaagttcct ttttcatcaa actattacct ctttcctcac
     7381 atttttttgt acttcatagt tttata
//
LOCUS       DAOSDD010000223         7040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3902_length_7040_cov_5.573801, whole genome shotgun sequence.
ACCESSION   DAOSDD010000223 DAOSDD010000000
VERSION     DAOSDD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_7040_cov_5.573801"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PK733_15730"
     CDS             complement(<1..876)
                     /locus_tag="PK733_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ12022.1"
                     /translation="MEKQTVAIDGIHIDVYSLNTVIVGTGCAGFNAADSLYSLGQKDI
                     AIVTEGINMGTSRNTGSDKQTYYKLTMAGSARDSVMDMAETLFEGGCMHGDIALIEAA
                     LSSRSFFKLVEIGVPFPHNSYGEYVGYKTDHDPKQRATSAGPLTSRYMTEKLQERVRE
                     RKIKIFDEYLVIGILTEKIAYNKEIINNRENNEIDNGVENKSSNSDAENKGSNNDAEK
                     KTIGLIALNLNALENPNMGFTLFNCKNIIYAVGGPAGMYSVSVYPESQTGASGIAFEA
                     GVKGVNLTESQYGIAS"
     gene            complement(1087..1926)
                     /locus_tag="PK733_15735"
     CDS             complement(1087..1926)
                     /locus_tag="PK733_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOJ12023.1"
                     /translation="MSLSNKTALITGAARGIGNAIAVRLAQDGYSVAMVATTDEEKIG
                     DSISGVREKGNPVIYIKGDISSEKSRKEIVSRVMNEFGRIDVLINNAGVAPKSRMDIL
                     ETTEESMDYVLNINLKGTFFLTQLVANTMINQIHNNNQIPDDSLIKGNKLIHNNFENC
                     CHPRIINISSISAYTSSTNRGEYCISKAGISMITALFADRLAEYGINVYEIRPGIIRT
                     DMTSAVKEKYDTLINGGLTPIKRWGYPEDIAKAVSVLCSGALDFSTGEVLNVDGGFHL
                     RRL"
     gene            complement(1971..3314)
                     /locus_tag="PK733_15740"
     CDS             complement(1971..3314)
                     /locus_tag="PK733_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOJ12024.1"
                     /translation="MEIEMEFCEFNYKSLEEVKSDVMKMNLDLHFSDNLDILRRTADI
                     NGKAIQNSIVFHPMEGTDGNYDGSPHELTIRRYERFSGGGAGLIWFEAVAVVPEGRVC
                     PRQLWITEDNVDEFKKLHTKIVENAHNEFGKDFTPVCIMQLTHSGRFSKPEGPPAPII
                     AYHNPYLDAVKRIDENIPVIEDEYIEMLEEKFEKAAVLAYKAGYDGVDIKSCHRYLNS
                     EFLAGFTRKGRYGGTFEGRTRFLLNTADRIRQRLGNKFIVTARLNIYDGIPYPYGWGV
                     DKDDYTKLDLTEPVKLIKLLYEKGVRMLNLTMGSPYYNPHVNRPFDKGGYIPEEHPLA
                     GVARLVGGIGEVQKAVPGMAIVGTGYSWLRQFSPYLAAGTLERGYATLIGFGRETFAY
                     PDFPKDIFNNGFVRKEKCCITCGKCTEIMRAGGTAGCVIRDSEVYGPIYKEYCRK"
     gene            3640..5274
                     /locus_tag="PK733_15745"
     CDS             3640..5274
                     /locus_tag="PK733_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ12025.1"
                     /translation="MSKFFSAIKNLLTYKESLEEQQFELLEYSENNEKGVERVAEQSY
                     ISLKLNENLAYINHRFNTIKSQDIIIREFKIGEKYTAFLVFIDEMVDKLTVNNSILRP
                     LMNTEAFQNIPSKEIVEYISNNVLSVGKISRENKYDSIVKQVLNGMTALFIDNCNEAI
                     LIGTKGFEKRNIEKPLTESVIRGAQEGFTEDLGTNIALIRRIIRNEDLIVEILPVGKA
                     NNSSCAIFYMDGITNPSLINEAKRRIESIDFDYISGSGMLEEMINDQPLLLFPQISTT
                     ERPDKSAIYLLDGKVLFIMDGAPFAGAVPTNFFDLIHSPEDYFLRWQFGTFLRFIRML
                     GIFASSILPGIYLALILFHHDMVPTDLLFSIAQSRENVPFPTFIELLLMEIAFELIRE
                     AGLRVPGAIGTTLGIIGALILGQAAVAASIVSPILIIVVAVTGIGSFATPSYTLAFAI
                     RIARFFFIVLGAAMGFYGISAAIVIILGIMCSMKSFGIPFFTPVAPKTNMRPDVIIGQ
                     PLYKQTERIEFTNPVDLKSAAAEPRGWKRRRQKDDK"
     gene            5264..6355
                     /locus_tag="PK733_15750"
     CDS             5264..6355
                     /locus_tag="PK733_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOJ12026.1"
                     /translation="MISEGKFGPQEAICLITISILSKIFFTSPRQVAEIVGTAGWYMT
                     LISAFIAAIGFTFVFFLLKKYKNKNIIEIYEIVYGKYIGIIISGLTGIFFLVNASVNV
                     REFGDVLKVYMLPITPLEYILIIFAGVILTLCIIGLESIARLSKLSSYFIFAGIIIVL
                     LLSVQNYDINHLFPLLGYGLGNTVLHGAARSSVYGDVFILAVIANSLQGINHIKKSGY
                     FSIIISGILASVNLLAFILAFPYSTAQEITSPMYELSALIDYGRFIQRIDPIFFFVWI
                     VSSFILITIYIYSFISIYCKMFKYPDLKPVLFPAIIMFLVIALIPEDVSTVVTGYIQS
                     IREYGWIIFYFIPFITFITSLIFKKEADA"
     gene            6357..7001
                     /locus_tag="PK733_15755"
     CDS             6357..7001
                     /locus_tag="PK733_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12027.1"
                     /translation="MLKMLNLNKFSLFKFLLLVAACILLITSLTSCTDSTEVNDMTYV
                     IVVGIDKGIINKWRLTVEFSTMKSNNGNSGGQTSDGKNGSSTGNASQNDYTTVTVDAP
                     SFFAGINIINTLVSRKLNFAHTKMIVISDEVAKDGLLGQFIAPIIRYRQMRRTSHVVI
                     SKGPAVEFVKACKLLIGKTLSTSIETIIEESQRSGFFPHVTLGRFYNNIKSFFY"
BASE COUNT         2099 a         1400 c         1189 g         2352 t
ORIGIN      
        1 agatgctata ccgtattgag actctgtcaa attaacaccc tttactccgg cttcaaatgc
       61 aatacccgaa gcaccggttt gactttccgg ataaactgat actgaataca tgcccgccgg
      121 tcctccgaca gcataaatta tatttttaca gttaaatagc gtaaaaccca tattaggatt
      181 ctcaagtgca tttagattaa gggctatcaa tccaattgtt tttttttcag catcattatt
      241 gcttcctttg ttttcagcat cactattgct acttttattt tctacaccat tatcaatttc
      301 gttgttttct ctattattga tgatttcttt attgtacgct atcttttcag ttaaaattcc
      361 aattaccaaa tactcgtcaa atattttaat ttttctttct cgaacccttt cttgaagctt
      421 ttcagtcata taccttgagg tcagaggccc ggccgatgta gcacgctgtt tgggatcatg
      481 atcggttttg tatcctacat attcaccata actattgtga ggaaatggaa ctccaatttc
      541 tacaagttta aaaaagctgc gtgaagaaag agccgcctct ataagagcaa tatctccatg
      601 catgcagcca ccctcgaaaa gagtttcagc catatccatt acggagtccc gggcactccc
      661 agccatagtt aatttataat atgtctgttt atcggagccg gtatttctgg aagtccccat
      721 gttaattccc tcggttacaa tggcaatatc tttttgtccc agcgaataaa ggctgtcagc
      781 tgcattaaaa cctgcacagc cggtaccaac tataacggta ttcaggctat acacatcaat
      841 atggattcca tcgattgcta ctgtttgttt ttccatatga acccctacct tccatgtata
      901 ctattctcaa ctatatttta ttttctctat tattctccca caacctcaga cttttctctg
      961 tctagctgcc cactggctgc tgcctgctgg ctactgtcca ttttcctccg tcctctatct
     1021 accatccacc atccactatc cactaatcac catccactat cctctatcta ccatccacta
     1081 tccagcttac aaccttctta agtgaaagcc tccatctaca ttcagcacct ctcctgttga
     1141 gaaatccagt gctccggagc aaagaacaga gactgccttt gctatatcct caggataacc
     1201 ccaccttttt ataggggtca gccctccatt tattagagta tcgtattttt ctttaacagc
     1261 agatgtcata tctgttcgaa taattcccgg tcttatttca tatacattta taccgtattc
     1321 tgccagtcta tcggcaaaca gtgcagttat catacttata cctgcttttg aaatacagta
     1381 ttctcctctg tttgtagagg atgtatacgc agaaatagac gatatgttta ttattctcgg
     1441 atggcagcag ttttcaaaat tattatgtat tagcttatta cccttgatta aactatcatc
     1501 aggtatttga ttattgttat ggatctgatt aatcatagta tttgcaacaa gctgtgtaag
     1561 gaaaaaagtc cccttaagat taatattcaa cacatagtcc atactttctt ctgttgtctc
     1621 aagaatatcc atccttgact tcggagcaac ccccgcatta ttaataagaa catcaattcg
     1681 accgaactca ttcataaccc ggctgactat ttcttttcta cttttttctg aagaaatgtc
     1741 ccccttgatg tagattaccg gattcccttt ctctcttacc ccggatatgc tgtcacctat
     1801 tttctcttca tccgttgtag caaccatggc tacagaatag ccatcctggg caagccttac
     1861 tgctatagca ttacctatac cccgtgcagc tccggtaata agagctgttt tattacttag
     1921 tgacacataa attccccctg tctatataaa tgcaggactg tccctggttg ttacttcctg
     1981 cagtattcct tgtatattgg tccatagact tctgaatctc ttataacaca cccggcagtg
     2041 cctccggctc tcataatttc agtacatttc ccgcaggtta tacagcattt ttcttttctt
     2101 acaaagccat tgttaaatat gtctttgggg aaatccggat atgcaaacgt ctcccttccg
     2161 aaaccaatga gagttgcata tcctctctca agagtacctg ctgccagata gggtgaaaat
     2221 tgtctgagcc aactgtagcc ggtcccaaca atagccattc cgggcactgc tttctgtact
     2281 tctccaatac caccaaccaa cctggctaca cctgccaggg gatgctcttc aggtatatat
     2341 ccacccttat caaaaggcct gttaacatga ggattgtaat aaggtgagcc catagtaagg
     2401 ttgagcattc tgactccttt ttcatataaa agttttatca gttttacagg ctcagtaagg
     2461 tccaattttg tatagtcatc tttatctacc ccccaaccat aagggtaagg tatcccatca
     2521 taaatattca accttgcagt aacaataaac ttattcccca gtctttgcct tatcctgtct
     2581 gcagtgttta gaagaaacct ggtgcgtcct tcaaaggttc cgccatacct tccctttctc
     2641 gtaaaacctg ctaaaaactc ggaattcagg tatcgatggc agctttttat gtcaacacca
     2701 tcatatccag ccttatatgc taatacagct gccttttcaa acttttcctc aagcatttca
     2761 atatattcat cttctattac aggaatattt tcatcgatcc ttttaacagc atccagataa
     2821 ggattatgat atgcaattat gggagcaggc ggaccttccg gcttgctgaa acggcctgaa
     2881 tgtgtaagct gcattataca aacaggagta aaatctttgc cgaactcatt atgagcattt
     2941 tcaacaatct ttgtatggag ttttttaaat tcatctacat tatcttctgt aatccacaac
     3001 tgtcttgggc agactcttcc ttcaggcact acggcaacag cttcaaacca tataagtcct
     3061 gctccgcccc ctgaaaacct ttcatacctg cgaatagtta attcatgtgg acttccgtca
     3121 taattgccat cagtaccttc cattggatga aaaactattg aattttgaat tgctttgcca
     3181 tttatatcag ctgttcttcg caaaatgtcc aggttgtctg aaaaatgaag gtccaaattc
     3241 attttcatta catcagattt aacttcctca agactcttat agttaaattc acagaattcc
     3301 atttcaatct ccattcctgc tacgtgcgaa atgaatttgc cattgttgaa caaaagtttt
     3361 ttataaaaag tttttatgga ctcaaattca ttttaccacc cgtgcaactt attttttttg
     3421 atatagtttg ccatctcaag atatggcttg ccatctcaat taactatctt tgctaataat
     3481 tataattata tcagttgcaa cctatgcaca tctatatatt atttgtgaca taattagcgt
     3541 tatttgtgtt tatatgtttt cacaatgtat agattggggc catgatgcca ataattgtac
     3601 ttagaaagct tatacaacaa attgcaccgg gaggtttaca tgagcaaatt ttttagtgca
     3661 ataaaaaatt tacttacata taaagaatca cttgaagaac aacaatttga gcttctggaa
     3721 tattcggaaa acaacgaaaa aggtgtagaa agggtagcag aacaaagcta tatatcccta
     3781 aagctaaatg aaaatcttgc ctacattaat cataggttca acaccattaa aagccaggat
     3841 attataatta gagaatttaa aattggtgaa aagtatacgg cattcttggt atttattgat
     3901 gaaatggttg acaaattaac agttaacaac tcaatactcc gtccgcttat gaatacagaa
     3961 gcatttcaaa atattccttc aaaagagatt gttgaataca ttagcaataa tgtgttatca
     4021 gttggcaaga tctccaggga aaataaatat gatagcattg ttaaacaagt gttgaatggt
     4081 atgactgctc tttttataga taattgtaat gaagcaatat taattggaac taaaggcttt
     4141 gaaaaaagaa atattgaaaa gccattgaca gaatccgtta taagaggtgc gcaagaagga
     4201 tttacagaag atcttggcac aaatatagct ttaataaggc gtattataag aaatgaagat
     4261 ttgattgttg aaattcttcc tgtaggaaaa gctaataatt caagctgcgc tattttttat
     4321 atggatggaa taacaaatcc gtctttaata aatgaagcaa agcgacgaat agaaagcata
     4381 gattttgatt atatttcagg cagtggtatg ctggaagaaa tgattaatga ccagccactt
     4441 ctgttatttc ctcagatttc tacaacagaa cgtcctgata aatctgctat atatttattg
     4501 gatggcaaag ttctgtttat tatggacggt gccccttttg caggtgccgt tcccacaaat
     4561 ttctttgacc ttatacattc tcctgaagat tactttttga ggtggcagtt tggaactttc
     4621 ctgaggttca taagaatgct gggtatattt gcttccagta ttctccctgg tatctatctg
     4681 gcgctgatat tatttcatca tgatatggta cctacagatt tgctgttttc tattgcacaa
     4741 tccagagaaa atgtcccttt cccaacattt attgagctct tattaatgga aatagctttt
     4801 gaattgataa gagaagcagg cttaagagtt ccgggagcta taggaacaac acttggtatt
     4861 attggagcct taatacttgg gcaggctgca gtagccgcca gcattgtcag ccctatactt
     4921 attatagtag tagccgttac aggtatagga agctttgcca ccccaagtta tactcttgct
     4981 tttgcaataa gaattgcgag gttctttttt atagtattgg gcgcagcaat gggattctac
     5041 ggaatttcag cagctatagt gattatactg ggcataatgt gcagtatgaa atctttcggt
     5101 ataccatttt ttacccctgt tgctcctaaa acaaatatga gacctgacgt aatcatcgga
     5161 caacctttat ataagcaaac agaaagaatt gaatttacta atcctgttga tcttaaatca
     5221 gctgctgcag aacctagggg gtggaaaaga aggagacaaa aggatgataa gtgaaggtaa
     5281 gttcggaccc caagaggcaa tatgccttat cactatatcg atcctgtcaa agatattttt
     5341 caccagcccc agacaagtgg cggagattgt aggtaccgca ggatggtata tgacactaat
     5401 atcagctttt attgcagcaa ttggctttac ttttgttttt tttctattaa aaaaatataa
     5461 aaataagaac ataatagaaa tctatgaaat tgtatatggt aagtatatag gcatcattat
     5521 atcagggctt acaggaatat tttttcttgt aaacgcatct gttaatgtga gagaattcgg
     5581 agatgttcta aaggtttata tgctgcctat aacgccgttg gaatacattc taattatatt
     5641 tgccggcgta attttaacac tatgtataat aggattggag tcaatagcaa gactatcaaa
     5701 gctgtcatcc tattttattt ttgcaggtat aatcatagta cttctgcttt cagtacaaaa
     5761 ctatgatatc aaccacttat tcccactatt gggttatggc ctgggaaaca cagtattaca
     5821 cggtgcagca agaagctcag tatatggtga tgtatttata ctggcagtaa ttgcaaattc
     5881 gctgcaagga ataaatcata taaagaagtc aggttatttt agcataatta tttccggaat
     5941 actagcttct gtaaatcttc ttgcctttat attggcgttt ccgtacagta ctgctcagga
     6001 aataacttct ccaatgtatg agctttccgc tctaatagat tatgggagat ttattcagag
     6061 aattgacccg atattctttt ttgtgtggat agtcagctct tttattctta taacaatata
     6121 tatatattca tttattagta tttattgtaa aatgttcaag taccccgatc ttaagcctgt
     6181 actttttcct gcaataataa tgtttctcgt tattgcacta attcctgaag atgttagtac
     6241 tgttgtaacc ggatatattc aaagtataag agagtatgga tggattatat tttattttat
     6301 cccatttatt acttttataa cttcattaat tttcaaaaag gaggctgatg cttaaaatgc
     6361 ttaaaatgct taatttgaat aagttttcat tgtttaaatt tttattgctt gtagctgcat
     6421 gcatcctttt aataacaagt ctcacgtcat gtacagactc aactgaggtg aatgatatga
     6481 cctatgtaat tgttgtagga atagacaagg gaataatcaa taaatggcgt ttaactgttg
     6541 agttttctac aatgaaaagc aataatggaa atagtggagg tcaaacatca gacggtaaaa
     6601 acggttcaag cacaggcaat gcatcgcaga atgattatac aactgtaacc gttgatgctc
     6661 cttccttttt tgcaggtata aatattatta atacgctggt atcaaggaag ttaaactttg
     6721 cacatacaaa aatgattgtt atttctgatg aagttgctaa agatggatta ctcgggcaat
     6781 ttatcgctcc tattataagg tatagacaaa tgaggcgaac atcccatgtg gttatatcaa
     6841 aagggccggc tgtggaattt gttaaggcat gcaaactttt aataggtaaa accttatcaa
     6901 cctccataga aacgataatt gaagaatccc aaagaagtgg ttttttccct catgttactc
     6961 tcggcagatt ttacaataat attaaatcct tcttctatta agtttaaatt cctttaaata
     7021 tctcctgttt tcttagtttt
//
LOCUS       DAOSDD010000224         7032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3912_length_7032_cov_5.469399, whole genome shotgun sequence.
ACCESSION   DAOSDD010000224 DAOSDD010000000
VERSION     DAOSDD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_7032_cov_5.469399"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..718)
                     /locus_tag="PK733_15760"
     CDS             complement(266..718)
                     /locus_tag="PK733_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOJ12028.1"
                     /translation="MVIDSIQNVFRYYGMGDTIKLALELLSETDFSKLQPGKYEVDGS
                     NLYYMVQNYKTRNIDECIWEAHKKYIDIQYIVDGQENIGYSNINQLAVKKQYDEISDA
                     VLLEGSGSIFTINSGFFVIFNPEDAHMPCISSNNNLSNVKKIVVKIKI"
     gene            complement(743..1783)
                     /locus_tag="PK733_15765"
     CDS             complement(743..1783)
                     /locus_tag="PK733_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12029.1"
                     /translation="MKVKWGVIGAGGIADRRTLPGMMLAKNAELVAVMEVNMELAEKL
                     RAKYNAKRAYDNVDAILADPEVEAVYIASPVIYHKDAAVKAAKAKKHILIEKPVALTA
                     DEGEKIAALCKEEGIKVAVGLMMRYHAYHQKMKEIIESGKLGKVVSCRAQLTCWYPDI
                     PGNWRQQKSTSGGGALMDMGVHCADLIQYITGGKATKVAALTGTKTFNYEVEDQASVI
                     FEMDNGAYAYVDSNFNIPDAAARCRFEIYGTKGSMLAEGTISQIEGGKLDVVLSDDSL
                     AYDAKQDRVDVSTVKIDVEFGNMYTKEIESFSNSILNNGKIEVPIEDALQVQRLIESA
                     YKSSEKGVFVNL"
     gene            complement(1887..2594)
                     /locus_tag="PK733_15770"
     CDS             complement(1887..2594)
                     /locus_tag="PK733_15770"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ12030.1"
                     /translation="MNVLAVGCHPDDLEIACAGTLAKYAKEGHNVFMCHVANGNMGHA
                     IIMPDELREIRTKEAERAGEIVGAKEVINLDVPDLKVNSYNEDTVMKLIEVIRYTKPD
                     IIITHGPQDYMKDHVETGKMVFDASFSSTIPHTFTKSSNRPGNVPIYYMDTLAGVDFL
                     PEEYVDVTDTIETKLNALNCHESQIKWMLEHDKIDFLDFVRTVSKFRGLQCGVKYAEG
                     FRRCVTWGRLITKRLLP"
     gene            complement(2696..3973)
                     /locus_tag="PK733_15775"
     CDS             complement(2696..3973)
                     /locus_tag="PK733_15775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ12031.1"
                     /translation="MAKEKEIVMNAAGVAELGFPMWPQFAEETFTDVLEPLKNGKVNY
                     WTGPKGMEFEKEWAKWIGAKYAISCTNGTAALHIAISSLGIGPGDEVIVPSYSFIASS
                     FSIVQAGAIPVFADVTEEHTIDPKAIEKLITPRTKGIVVVHLYGIVADMGPILEIAKK
                     NNLKIIEDCAQCFGGEFNGIKAGMIGDVGCFSFCQSKHFTTGGEGGMVVTNDENLAWE
                     CRSFRDHGYDVRTRMNLLALEEKLPYIHHRVGFNYRMTEIQSVIGLNELKRFDSWNLA
                     RRKKYAKIYDEAFIGLEGIKAVPVNTEERKNAYWWYPVLLDIDKLDCNAEKFIKDLQA
                     EGIPCYGIQWPEAYEEKAYKELNGFGEAKFPFKSTEYTNPESVHYEKVVCPMAKYLKQ
                     ETVSLFLHPSWEECHIQRCIDAVKKVLDQHKKK"
     gene            complement(4060..5391)
                     /locus_tag="PK733_15780"
     CDS             complement(4060..5391)
                     /locus_tag="PK733_15780"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ12032.1"
                     /translation="MKAAAKSIDITPAVGIPMGGNVREDDKARGIHDNLYCNAILLEE
                     NGKEICFLGFDLLGLNFETCRNIKESIREKTGLATENIVINATHTHSGPDTLGIFKES
                     MDESCRKYIEEMVDKVSREVSLLPDELKEANLAIARNSVYELSFNRRLVMKDGSMRMN
                     WEDVNIEDVLKETGPIDPELNVLMIEDNNKGAIAVIVNFTLHPAVLVGKDWLWSRDYI
                     NYLTLELKNKLGGDIVVLFANGSEGNVNHINYKDKNQGRGFEEAERIGSILAKHVIET
                     LNNKKSLKSKQFKSLSRNIKLPLRTITNESVQWAEKLLKEKGDFVPSLLDGVPDEIYA
                     REILKLSKVKDKSIETEIQVIQIDDTVIATLPGEVFVEFGLKIKKESPFKNTLVFGLA
                     NDMIGYIPTEISFEEGGYEIKTAWSSKLDPRAGDVLASQVLDLIKEISNLT"
     gene            complement(5513..6868)
                     /locus_tag="PK733_15785"
     CDS             complement(5513..6868)
                     /locus_tag="PK733_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ12033.1"
                     /translation="MLKLKKNKREEKQAANQQLVKETNLTLIFNLINRYGPVSRADLA
                     HITGLSPTTVSSLAEELIKNDIIIETGEGTTATSGRKPIMLEVNPAGGYIASIEMIES
                     GFIFSLYDLKCSEVYEKKYKVSNYNSIGLIIAEIVGEQLRTLGIEENKLFGICLGVPG
                     LIDVEKNCVITSTVIPIDENNNFYHYIKDRFKEIPVFLGNESWFSAYAEKEFGIDSDI
                     RNLIFIDINVGVGAGIILNGQIFTGFLGLAGEIGHMSIDMDGPVCKCGNRGCLETMVS
                     IPAIIRNIIYEIESGKNSVLKEMSKCCITKAFKPNKTSDPNNISNVNNVINNVEINPN
                     NGMYANGIDISLIQDAFERKDDLVMNIIELTARRLAFGITNIINLVNPEVVVIGGEIT
                     KLGPAFLEKTTHYLENMQLKPNKHKIKVKYSTLKGNTVTLGGAKYVLDNILNTGILLE
                     V"
BASE COUNT         2039 a         1539 c          995 g         2459 t
ORIGIN      
        1 agatgcatca tatatatttc ccgggtactt aaaatgctga agcagcatat tgcgctttaa
       61 tggcggtacc ttatcaagag aacttaacca aacccaatat ttaatatcgc cactagtata
      121 cttttggctt ttacatcctg ggttttcatt tttttcaaca aacatacata tcaccccaaa
      181 taaaaatggt ataagaatag tgctaaacac gaagttatgc acacttaact tataccatat
      241 tttggctata cttgcaatac atgttttata ttttaatctt aactacaatc tttttaacat
      301 tgcttaaatt attgttactt gatatacacg gcatatgtgc atcctcagga ttaaatatta
      361 caaaaaaacc tgaatttata gtaaaaatgc tgccgcttcc ttccagtaaa actgcatctg
      421 atatctcatc atattgtttt tttacagcta actggttaat atttgaataa ccaatgtttt
      481 cctgcccgtc cacgatatat tggatatcaa tgtatttctt atgtgcttcc cagatacatt
      541 catcaatgtt ccttgttttg taattctgca ccatgtaata taaattgctt ccatctacct
      601 catattttcc aggctggagt ttggaaaaat ctgtttcaga taaaagctca agagccagtt
      661 ttattgtgtc tcccattcca taatagcgaa aaacattttg aatactgtct ataaccatag
      721 aaaccctccc tatccgaaaa tattacaggt ttacaaaaac tcccttttca gaagacttat
      781 aagcagattc tatcaatctc tgaacttgaa gagcgtcttc aattggcacc tcaattttac
      841 cgttattaag aattgagttg ctgaaagact ctatttcttt agtatacatg ttgccaaatt
      901 caacatcaat tttcactgta gatacgtcaa ccctgtcctg tttggcatca tatgcaagag
      961 aatcatctga aagtactaca tcaagttttc cgccttctat ctggcttata gtgccttctg
     1021 caagcatgct tccttttgtt ccgtaaattt caaatctgca ccttgcagca gcatccggta
     1081 tattaaagtt ggaatctaca taagcatacg cgccattatc catttcaaaa ataactgatg
     1141 cctgatcttc tacctcatag ttgaaagttt tagtgcctgt aagagctgca accttggtag
     1201 ctttcccacc tgtaatatac tgaataaggt cagcacagtg tacccccata tccataaggg
     1261 ctccgccacc agaagtactt ttctgctgtc tccagtttcc aggtatatca gggtaccagc
     1321 aggttaactg agctctgcac gatactactt ttccaagttt tcccgattca ataatttcct
     1381 tcattttttg atgatatgca tgatatctca tcataaggcc gactgcaacc tttattccct
     1441 cttctttaca taaggcagca attttttcac cttcatctgc cgttagagca acaggcttct
     1501 caataagaat atgctttttt gctttggcag ctttaacagc cgcatctttg tgatatataa
     1561 ccggagatgc aatatatact gcttcaacct cagggtctgc caatatggca tcaacattat
     1621 catatgcccg tttagcgtta tatttggctc ttaatttctc agccagttcc atatttactt
     1681 ccattactgc aaccaattca gcgttttttg caagcatcat tccaggcaag gttcttctgt
     1741 ctgcaatacc tcctgctcct attacgcccc atttaacctt cataataaaa cctccttttt
     1801 ttataactgg cactgcccga attatattga aatatatcga ttaattcaag acagctgcca
     1861 ttttaaatta ttgcccagct ttcaatttac ggaagcaatc tctttgttat aagtctgccc
     1921 caggttacac agcggcggaa tccttccgca tatttcacgc cacactgcag acctctgaat
     1981 ttagatactg tgcgtacaaa atcaagaaaa tcaattttgt catgttcaag catccattta
     2041 atctgacttt catggcaatt taaagcattt agctttgttt ctatagtatc tgtaacatca
     2101 acatattctt caggtaaaaa gtcaactccg gcaagtgtat ccatataata gataggaaca
     2161 ttaccagggc gattagagct ttttgtaaat gtatgaggaa tggtggagga aaaactggca
     2221 tcaaatacca ttttgcctgt ttccacatga tccttcatat aatcctgagg cccatgagta
     2281 attattatat caggtttcgt ataacggatt acttcaatta atttcataac cgtatcttcg
     2341 ttatacgagt taaccttcaa atccggtaca tcaagattaa taacttcctt tgctccaaca
     2401 atttcccctg ctctctcagc ttctttagtt ctaatctccc ttaattcatc aggcataatt
     2461 atagcatggc ccatattacc attggctaca tgacacataa atacattatg cccttccttt
     2521 gcatattttg ccagagtacc tgcacatgct atttccagat catctggatg acatccaacc
     2581 gccagtacat tcataatact acctcccaaa ttgttagtat aaccctatca agggtgtgac
     2641 ataatgaatt ttgatgctgc acacccctga atattatggt aatttatttt gcggcttatt
     2701 ttttcttatg ttgatccaga actttcttca ctgcgtcaat acatctttga atatgacatt
     2761 cttcccatga aggatgcagg aacaagctta cagtttcctg cttcaggtat ttcgccattg
     2821 gacaaacaac cttttcataa tgtacagatt cagggttggt gtattcagta gacttaaacg
     2881 ggaatttggc ttcaccgaaa ccattcaatt ccttataagc tttttcctca tacgcttcag
     2941 gccactgaat gccatagcat ggaatacctt ccgcctgtaa atccttaata aatttctctg
     3001 cattacaatc cagcttatca atatccaata ataccggata ccaccaataa gcattctttc
     3061 tctcttctgt attaacagga acagctttta ttccttccag accaataaat gcctcatcat
     3121 atattttagc atattttttg cgtcttgcaa gattccacga atcaaatctt tttaattcat
     3181 taaggcctat tacagattgt atctctgtca ttctgtaatt aaaacctact ctatgatgga
     3241 tatatggtag tttttcctca agtgctaaaa gattcatacg tgttcttaca tcgtagccat
     3301 gatctctaaa agagcggcat tcccaggcaa gattctcgtc attggttaca accattccac
     3361 cttcaccgcc ggttgtgaaa tgtttgctct gacaaaagct aaaacatcct acatcaccaa
     3421 tcatacctgc ttttataccg ttgaattcac caccaaagca ctgtgcacag tcctcaataa
     3481 tcttaagatt attctttttt gcaatttcca atatgggtcc catatctgct actatgccat
     3541 acaaatgtac tacaacaata cccttggtcc tgggagttat taacttctct atagccttgg
     3601 gatcgattgt atgttcctct gtaacatcgg caaataccgg tattgctcct gcctgtacta
     3661 ttgagaagga tgatgctatg aaagagtatg agggtactat aacctcatca ccggggccta
     3721 tacccaaact gctgattgca atatgaagtg ccgcagtacc attggtacat gaaattgcat
     3781 attttgcacc aatccatttt gcccattcct tctcaaattc catccccttt ggccctgtcc
     3841 agtaatttac ctttccattc tttaaaggct ccaatacatc tgtaaaagtt tcctctgcga
     3901 actgaggcca cattgggaat cccaattcag ctacacctgc tgcattcata acaatttcct
     3961 tttcctttgc catacttaac ccaccttttt aaatattttt tgttttgtgc ttttataaaa
     4021 gcaatgtatt tatacaaacc gccatgcggt ttatatattt cacgtcagat tacttatttc
     4081 cttaataagg tctaatactt gcgaagcaag aacatcaccc gctctaggat caagcttgct
     4141 tgaccatgct gttttaattt catatccgcc ttcttcaaat gaaatttctg ttggaatgta
     4201 gcctatcata tcattagcaa gcccgaaaac cagtgtgttt ttaaatggtg attctttttt
     4261 tatttttaga ccaaactcta caaacacttc tcccggcaag gttgcaataa ctgtatcatc
     4321 tatctgaata acttgaattt ctgtttcaat gcttttatct ttaactttag atagcttcag
     4381 aatttctctg gcataaattt catcgggtac tccatcaagt aatgaaggta caaaatctcc
     4441 cttttctttc agtagtttct cagcccactg taccgattca ttagttattg ttcttaatgg
     4501 cagtttaata tttctcgaaa ggcttttaaa ctgttttgat ttcagagatt tcttgttatt
     4561 aagtgtttct attacatgtt tagctagaat acttccaatg cgttctgctt cttcaaatcc
     4621 tctcccttga tttttgtcct tataatttat atggtttaca tttccctctg aaccattggc
     4681 gaaaagtaca acaatatcgc caccaagttt gttctttaat tccagagtaa gataattaat
     4741 ataatctctt gaccatagcc aatccttacc tacaagaact gccggatgaa gagtgaagtt
     4801 aacaattact gcaattgcac ctttgttatt gtcttcaatc attaatacat tcagttcagg
     4861 atctataggg cctgtttctt tcaaaacatc ctcaatattt acatcttccc agttcatcct
     4921 cattgatccg tctttcataa caagccgcct gttaaaactg agttcataaa cactattcct
     4981 ggcaatggcc aggttggctt ctttaagttc atccggcaat aatgaaactt ccctggagac
     5041 tttgtcaacc atttcctcta tgtattttct gcaagattca tccatgcttt ccttgaatat
     5101 tcccaatgta tccggaccgg aatgcgtatg agttgcattt atcacaatat tctcagtagc
     5161 cagacctgtc ttttcccgta tactttcctt tatattcctg caagtctcaa aatttaatcc
     5221 caaaagatca aatccaagaa aacaaatctc ttttccattt tcctcaagaa gaattgcatt
     5281 acagtaaaga ttatcatgga tacctcttgc tttatcatct tctcttacat taccgcccat
     5341 tggtattccg acagccggag taatatctat actttttgct gctgctttca tgtaaacctc
     5401 ccccttatca ttggaattat aatttaaata cgattattta tcgtaaaaat atcaattata
     5461 ttattccttt gcccgcataa aaatgttatg cagtattaaa atttataaat tcctatactt
     5521 ccagcaatat cccagtattt aaaatgttgt cgagtacata ttttgcaccg ccaagagtta
     5581 ctgtatttcc tttaagcgta gaatatttga ctttaatctt gtgcttgtta ggcttcaact
     5641 gcatgttttc cagataatgt gttgttttct caaggaaggc aggtcctagt ttggtaattt
     5701 caccgccaat aacaacaact tccggattaa caagatttat tatgtttgta atcccaaaag
     5761 ccagccttct tgctgtaagc tcaataatgt tcataacaag atcatctttt ctttcgaagg
     5821 catcctgaat taaacttata tctattccgt tcgcatacat tccattattg gggttgattt
     5881 caacattatt aataacatta ttaacgttac taatgttatt aggatcagat gttttattag
     5941 gtttaaatgc cttggtaata cagcatttgc tcatttcctt caatacactg tttttaccgg
     6001 actcaatttc atatataata tttctgatga ttgccggtat actaaccatt gtttccagac
     6061 atcctctatt cccacattta caaactggtc catccatatc tattgacata tgaccgattt
     6121 cccctgcaag tccgagaaaa cctgtaaata tctgaccgtt caaaattata cctgcaccaa
     6181 ctccaacatt tatatcaata aaaataaggt ttcggatatc ggagtcgatc ccaaactcct
     6241 tttctgcata agcagaaaac caggattcgt tacctaaaaa taccggtatt tctttaaacc
     6301 tatccttaat ataatggtaa aaattattat tttcgtctat agggattaca gttgaagtta
     6361 ttacacagtt cttctcaaca tcaatgagtc ccggtacccc aagacagatt ccaaaaagct
     6421 tattttcttc aatacccaat gttctcaatt gttctcctac gatttctgca ataatcaatc
     6481 ctatactatt ataattggat actttgtatt ttttttcata aacttcacta catttcaaat
     6541 catacaatga aaaaataaat cctgattcaa tcatttcaat agatgctata taaccgcctg
     6601 caggattaac ctcaagcatt attggctttc ttccgctggt agcagtagtt ccctcacctg
     6661 tttctataat aatatcattt tttataagtt cttcggccaa cgacgatact gtagtaggac
     6721 ttaatccggt aatgtgtgcc aggtcagctc ttgacaccgg tccataacgg tttatcaaat
     6781 taaatattaa tgtgagatta gtttctttaa caagctgttg atttgcagct tgtttttctt
     6841 cccgtttatt cttttttaat tttaacattt atatgtttct ccagtgaatg aactaattat
     6901 atataaaaaa actatctaaa attcctttat tttatgataa cacttcatta ataaaaaatc
     6961 aagtggtttt tagaagttgg gcgcatcttc tatatttgaa gaaaaatatg gcaagaaacg
     7021 atttccatgt at
//
LOCUS       DAOSDD010000225         7027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3916_length_7027_cov_3.354705, whole genome shotgun sequence.
ACCESSION   DAOSDD010000225 DAOSDD010000000
VERSION     DAOSDD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3916_length_7027_cov_3.354705"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK733_15790"
     CDS             complement(<1..270)
                     /locus_tag="PK733_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12034.1"
                     /translation="MRKRYTKDFYSRRLLIFTAIFISIFLSGTIISGTYGSVFASEND
                     AFEPEYFKGVMDMVKGKYAGEVEDRLLIEGALKGMLGTMDPYTTYF"
     gene            complement(421..1968)
                     /locus_tag="PK733_15795"
     CDS             complement(421..1968)
                     /locus_tag="PK733_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ12035.1"
                     /translation="MKKASTFIVFIVTVFCILQPMTVFAQKVELGYEGGISSGTKDEK
                     TPLEYSEVCFLTGEPIVFSGTVSVKKTTKQDTTNATYTYNLKNIERSATLTRSMSYNI
                     RTVKTEKGQIIEELSLSRNPSETIRINNDIYVLRNYEFSNSEIIDKRPAIDYSAGNMW
                     GRKTYQAGNANNGGTVTVEITGKIFGYDHYWGNTETAILNYEINSERKNGDILDRWGG
                     NAKVIISSSIIKEIKFMKNIPEQISFEGGYVRTYNNSSMLEYYCCLPEFDSKGISNYR
                     MVETRDILKLEALPEQTRLPVYDLSYLRGHWAEKEINILHSLGVFNDKEKKFNPEAFI
                     TRGEFIAALVEVFWDNSINPSINERITASGSTRTRRGKDKAEEIISPYKDIPTDHRYF
                     PQIGKAHKNGLVTGRGQDIFAPEDTISLAEIMVIFVRALGLEDLAPNPYAITSFQDDI
                     SIPAYARNAIYAADRMGLIEGDNRGYLKPDEKITKAKAAILLNNFITYMREGIRKDYQ
                     ECIIGFN"
     gene            complement(2025..3761)
                     /locus_tag="PK733_15800"
     CDS             complement(2025..3761)
                     /locus_tag="PK733_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12036.1"
                     /translation="MYKYMRTHRYICSTCIALLVYFLLFIPANNACASSASDYENYDL
                     NKITVVSGIVEDNNPILGYITIYNPDFVENGAKIQDRQDMLRTYNYLKADKVEVLKNH
                     KKSSLESIVEGDTVFIRTDERGYVASVSGVSNCVAVFGKILYKSPDTLIIEYQSDKSQ
                     EILKISDNIQVFINGILSRYDDLEEGDRIRLLLHKALGLTRVKEIIVEEGGRNLISNI
                     YKASISYIDDISNRIITRNLLAFEGGKWVRTGIRGFAGFELKDDCNIFYNGREVDTNS
                     VRLILKNSEAYIVAEKYFGGREKIKLISVRNKEEAEVIYDDIVENILPGSERFIINKE
                     LKSITYDNQSIVIKDGRLVTGKNISENDTAYIVAGRSNMGDYKAGIIQVYHKPAVDNL
                     QIYRGRIKSINENTDFTVESFSRLDDINWGFNNTAKTFGINFDTRILDEEGLVNQDNF
                     TGYGENSYINRTVYIVANSLDALIISTAPYGAYNIRGEVYKTGVSTAEEENTNTGEPD
                     EIYLFKARVYDITIHKWRDSGEMNLKLLKNSIILKDGKEIKPSDLKKGDSVRVIKKDK
                     TKTGDTYIIFVE"
     gene            complement(3967..4593)
                     /gene="sleB"
                     /locus_tag="PK733_15805"
     CDS             complement(3967..4593)
                     /gene="sleB"
                     /locus_tag="PK733_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HOJ12037.1"
                     /translation="MRDIGRLWGIPDNNRETLSYYGSRGQEVINIQTRLKKWGYYKGD
                     IDGIFGSQTYQAVRYFQSKNGLKVDGIAGPETLAALGLPTGQTTSQSNDKDVNLLAHI
                     IHAEARGEPYSGQVAVGAVVLNRVRDSRFPGTIAGVVYQPGAFDAVYDGQINLQPDTA
                     AYNAARDALNGWDPTYGCVYYWNPATATSSWIWSRQIVVTIGKHVFAR"
     gene            complement(5003..6709)
                     /gene="argS"
                     /locus_tag="PK733_15810"
     CDS             complement(5003..6709)
                     /gene="argS"
                     /locus_tag="PK733_15810"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ12038.1"
                     /translation="MINVIERMKQDIEYTVSQSIGKAAKNKQLPQIDTCAIFIETPRE
                     KGHGDFSTNVAMQMTRQAKMPPVKIASIIIENIIESLKFEDTYIGKVQCAGPGFINFY
                     LKNDWLYYTLKVIQSERENYGRLDVGHGKKIMVEFVSANPTGPLHMGNARGGALGDCI
                     ASVLEAAGYDVTREFYVNDAGNQMDKFGISLEARYIQLLRGKNSILFPEDGYHGDDIT
                     EHAKKYIEVYGDKLLDYSSDERRNELVKYALEKNLESIKSVLEDYGVRFDVWFSEQSL
                     YDSGELKDTLEYLEENELTYEKDGALWFKTTGFGTDKDEVIVRSNGMPTYFASDIAYH
                     RNKFLKREFDRVINLLGADHHGHVARMKAAVEALGVEPDKLDIIIFQLVRLYRNGEIT
                     RMSKRTGKAISLADLLDEAGKDAARFFFNMKASGNHLDFDLDLAVRQSNENPVFYVQY
                     AHARICSMLKLLESEGIRVPDVEQVDLTPLKENEEIELIKKLAEYPEEVGISAQTLEP
                     SRLTRYVMDVASLFHSFYNACRVKGEEENLMMARLILVDSTKIVIKNILDLLSITAPE
                     KM"
     gene            complement(6693..>7027)
                     /locus_tag="PK733_15815"
     CDS             complement(6693..>7027)
                     /locus_tag="PK733_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HOJ12039.1"
                     /translation="YVTYNESEVTGMDGTTTTIKVCDGIVTLMRVGSVNSQFVFQKGR
                     KHISYYDTTYGVFTVGVFASEVNVSMDDCGGEISVDYHIEIDNNKTGYNDFYMSIREV
                     GCLNDKCY"
BASE COUNT         2070 a         1541 c          979 g         2437 t
ORIGIN      
        1 gaaatacgtg gtataagggt ccatcgtgcc caacattccc tttaatgccc cttctattaa
       61 tagtctatct tctacttcac ctgcatattt tcccttaacc atgtccataa cacctttaaa
      121 atattcaggt tcaaaggcat cattttcaga cgcaaaaacg cttccgtagg ttccagatat
      181 tattgtacct gataggaata tagagatgaa aatagctgtg aaaattagaa gtcttcttga
      241 ataaaagtct tttgtatatc tttttctcac atgaattctc cttttccaat aataaaatta
      301 taaatctgct gaaaagcaaa tttataattt tattttacca cagtttaaat attttttgta
      361 tgaaaaaaat atttatgagg cgcgcatttt ctatttctga tacataatta ggtgtattta
      421 tcaattaaag cctattatgc actcctggta atcctttctg ataccttctc tcatgtaggt
      481 aataaagtta tttaataaaa tagcagcttt agctttggta attttctcat caggctttaa
      541 atagcctctg ttatctcctt cgataagtcc cattctatct gctgcgtaga ttgcgtttct
      601 tgcataggca ggtatactta tatcatcttg aaaagaggta atggcataag gattgggagc
      661 taaatcttcc aatccaagag ctctcacaaa aataaccatt atttcagcca atgaaatcgt
      721 atcctccggg gcaaatatat cctgccctct tccagtaacc agtccgttct tgtgagcttt
      781 gccgatttgg ggaaaatacc tatgatctgt tggtatatct ttatatggag aaataatttc
      841 ttctgcctta tcttttcctc ttcttgtcct tgtacttccg gaagcagtta ttctttcatt
      901 tatggaagga ttaatagagt tatcccaaaa aacctcaaca agagctgcaa taaattcccc
      961 tcttgtaata aaagcttcag gattaaattt cttttcttta tcattaaaca ctccaaggct
     1021 atggagtata ttaatttcct tttcagccca gtgacccctt aaatacgaaa ggtcatatac
     1081 cggtagcctt gtttgttccg gcaaagcttc aagttttaaa atatctctcg tttcaaccat
     1141 cctgtaattc gagatacctt ttgaatcaaa ttcaggaagg caacaataat actcaagcat
     1201 gcttgaatta ttgtaagtcc tgacatagcc accctcaaaa cttatctgtt caggtatatt
     1261 tttcataaac ttaatttcct taataattga agatgagatg atcacttttg cgttgccgcc
     1321 ccatctgtcg agaatatcac cattttttct ttcgctgttt atttcataat ttaatatagc
     1381 cgtttcagtg tttccccaat aatgatcgta tccaaatatt ttacctgtaa tttcaactgt
     1441 tacggtacca ccgttgtttg catttccagc ctgataggtt ttccttcccc acatattgcc
     1501 tgcagaatag tctattgcag gtcttttatc aataatttca gaattggaga actcataatt
     1561 cctcaacaca tatatatcat tattaatcct tatagtctca gatggatttc tcgaaagtga
     1621 caattcttca attatctgac ccttctcggt ttttacagtt ctgatattgt atgacatact
     1681 ccttgtaaga gtagcacttc tttcaatatt tttcaaatta tatgtataag tagcatttgt
     1741 agtatcctgc tttgttgtct tttttacact aacagtacct gaaaatacta taggctcccc
     1801 tgtaaggaaa catacttcgc tgtactcaag aggagttttc tcatctttgg ttcctgaaga
     1861 tatcccaccc tcataaccca attcaacctt ttgtgcaaac actgtcatag gctgcaatat
     1921 gcaaaaaact gtaactatga atacaataaa cgtcgaagct tttttcatcc tgccaaaccc
     1981 tcctaaattc caaccttcaa cctctctcct ctaacttcta gcttctattc cacaaaaata
     2041 atatatgtat ctcctgtttt ggttttatcc tttttaataa cccttacact atcacccttc
     2101 tttaaatctg aaggtttgat ttcttttccg tcttttaata ttatgctgtt tttcagtaac
     2161 ttaaggttca tttctccact atctctccat ttgtgtatag taatatcata gaccctggcc
     2221 ttaaacaaat aaatttcgtc aggttccccg gtatttgtgt tttcttcctc tgcagtactg
     2281 acaccagtct tgtacacttc tcccctaata ttatatgccc cataaggagc tgtacttata
     2341 atcagagcgt caagactatt ggcaactata tagactgtcc tgttaatata gctgttttct
     2401 ccgtatccgg taaagttatc ctggttaacc aaaccttctt catctaatat tctcgtatca
     2461 aaattaatac caaatgtttt ggcggtattg ttaaaccccc aatttatatc atccaatcgt
     2521 gaaaatgact ctactgtaaa gtctgtattt tcgttgattg acttaatcct gccacggtaa
     2581 atttgcagat tatccacggc cggcttatgg tatacctgaa taataccggc cttataatct
     2641 cccatattgc ttctaccggc tactatatat gcagtatcat tttcagaaat gttctttccc
     2701 gtaaccaacc ggccatcttt aataacaata ctttggttat catatgtaat actctttaat
     2761 tctttattaa taataaacct ttctgatccc ggtaaaatat tttccactat gtcgtcataa
     2821 attacctctg cttcttcttt attcctgaca gaaattagtt ttatcttttc acggcctcca
     2881 aaatattttt ctgctacaat atatgcttca ctatttttta gtataagtct tacagaattt
     2941 gtatcaactt ctctgccatt ataaaatata ttgcaatcat ctttcagttc aaaaccagca
     3001 aaacctctaa tacccgtcct tacccacttc cctccttcaa aagcaagcag attcctggta
     3061 ataatcctgt ttgatatatc gtcaatataa cttatagaag ctttgtatat attagatatt
     3121 aaattccggc cgccctcttc gacaataatt tcttttactc ttgtaagccc cagcgcctta
     3181 tgcaaaagaa gccttattct atcaccctct tccaggtcat catacctaga tagtattcca
     3241 tttataaaaa cttgaatatt atcactgatt tttaatattt cctgtgattt atcagattgg
     3301 tattctatta ttagggtatc aggagattta taaagtattt taccaaaaac tgcaacacag
     3361 ttgctcactc cgcttactga agcaacatat cctctttcat ctgtccttat aaatacagta
     3421 tcaccttcca caatgctttc aagagacgac tttttatggt tttttaacac ctctactttg
     3481 tctgctttca aatagttata tgtacgaagc atatcctgcc tatcctgaat ttttgcacca
     3541 ttctcaacga aatccggatt gtatatggta atatagccta gtatgggatt gttgtcttct
     3601 actataccac ttactacagt aattttattt aaatcataat tttcataatc actggcagat
     3661 gaggcgcatg cattatttgc cggtataaag agtaaaaagt atactaacaa agcaatacat
     3721 gtactacata tatatctgtg tgtcctcatg tatttataca taaattaact cccccttact
     3781 ttagttaatt tcccctattg ccgttaactg ccattgtaat tgctgatata agcaataagt
     3841 tgcaaaaagt agtaaaaaac aaccactaaa aaaaatgtct tatattattt atcggcaact
     3901 aaatccgatt atttaatagt atatattcca cttataatag tatatatttc acttaattgc
     3961 ggcgtatcat cttgcaaaaa catgtttccc tatagttacc acaatttgtc tgctccagat
     4021 ccagctgctt gttgcagtag caggattcca gtaatataca cagccatagg ttggatccca
     4081 gccgtttaag gcgtcccttg cagcattata tgcagctgtg tcaggctgca agttaatctg
     4141 accgtcatat actgcatcaa aagctccggg ctgataaaca acaccagcaa tagtgccagg
     4201 aaacctactg tctctgactc tatttaaaac cacagcacca acggctacct gtcctgaata
     4261 tggttctccc cttgcttctg catgtattat gtgagcaagt aaattcacat ccttgtcatt
     4321 agattgagat gtagtctgtc ctgtaggcag tccgagagct gccagcgttt caggacccgc
     4381 aataccatca accttcaacc cattttttga ttgaaaatac cgtactgcct gatatgtctg
     4441 agagccaaaa attccatcaa tatcaccttt ataatacccc cactttttta gtctggtttg
     4501 aatatttatt acttcctgtc ccctggaacc ataatatgaa agtgtttccc ggttattatc
     4561 cggtatgccc cataatctgc caatatcccg caaattggca ttcgaaagat aaaacactcc
     4621 aaaaaacaaa gacagtacta aaataactac taccaaaatt aggtatttct tcctcaaaaa
     4681 tgtttcactc cttctatgct attctccatc aattttgaca gataatagta ttgactataa
     4741 gtatattttt tactaaccga ctaaaaatat tcaaaagaat gagtttgtaa agagtgtgtt
     4801 tataaaagtg aagaaataat tagggcatat ttttcttttt tgaagaaaaa tatgcccgac
     4861 aatataggtt ttcttagcgc atgttcgact cagcaagggc gtattttttt tatatgacca
     4921 aaaaaagaaa atgtgaccct tcagcaacgt attgtaatat ttaagtcatg gggaaaattc
     4981 tacccattac atttaccatc aattacattt tttcaggagc cgtaatgctt aataaatcaa
     5041 ggatattctt aatcacaatt ttagtactat ccaccaatat aagccttgcc atcataagat
     5101 tttcttcctc gccctttact ctgcatgcat tgtaaaagct atggaaaagt gaagctacat
     5161 ccatgacata tctggtaagt ctgctaggtt ccagtgtttg ggctgatatc cctacttcct
     5221 cggggtattc cgccagcttt tttatcagtt caatttcctc attctcttta agaggagtca
     5281 aatcaacctg ttcaacatcc ggaactctta taccctcgct ctccagtagt ttcagcatac
     5341 tgcatatcct tgcatgggca tattgtacat agaatacggg gttttcatta gattgcctca
     5401 cagccagatc cagatcaaaa tccaaatgat tccctgaagc tttcatgtta aagaaaaacc
     5461 ttgcggcatc tttacctgct tcatcaagca agtctgcaag ggatatggct ttgccggtcc
     5521 ttttagacat cctggtaatt tccccatttc tatataagcg tacaagctga aaaattatta
     5581 tatccagttt gtcgggttca actcccagag cttctaccgc tgctttcatt ctggcaacat
     5641 gtccatggtg gtcagcacca agcaaattta taaccctatc aaattcacgt tttaaaaact
     5701 tgtttctgtg atatgcaata tctgaagcaa agtatgtagg cataccatta cttctgacta
     5761 taacttcatc tttatctgtt ccaaagccgg tagttttaaa ccataaagct ccatcctttt
     5821 cataagtcag ctcattttcc tcaaggtact ccagagtgtc tttcaactct ccgctatcat
     5881 acaaggattg ctcggaaaac catacatcaa acctaacccc atagtcctca agaacacttt
     5941 ttatactctc aagatttttt tccaaagcat atttaaccag ttcattacgc ctttcatcag
     6001 aagaataatc cagaagctta tctccataca cctctatata tttttttgca tgttctgtaa
     6061 tatcatctcc atgatatcca tcttccggga ataaaatgga attttttccc cttagtaact
     6121 gaatatacct tgcttcaagg gatattccaa atttatccat ctggttccca gcatcattca
     6181 cgtaaaactc cctggtaaca tcataccccg cagcctccag aacactggct atacaatcac
     6241 ctaatgctcc tcctctagca tttcccatat gcaatgggcc tgtaggattt gcacttacaa
     6301 attccaccat aatttttttg ccatgtccaa catccagtct gccatagttt tccctttcag
     6361 attgaataac ctttaaagta tagtataacc aatcattttt aaggtaaaaa ttaataaaac
     6421 caggaccggc acactggact tttcctatat aagtatcttc aaatttaaga ctttcaataa
     6481 tattttcaat aataatagaa gctattttaa caggaggcat ctttgcctgc cgagtcatct
     6541 gcatggctac atttgttgaa aaatctccat gtcccttttc cctgggagtc tcaataaata
     6601 tagcacaggt atcaatttga ggtaattgct tgtttttagc agctttacct atggactggc
     6661 ttactgtgta ctcaatatcc tgtttcattc gttcaataac atttatcatt caagcatcct
     6721 acctctctaa ttgacatata aaaatcatta taccctgttt tattgttgtc tatttcaata
     6781 tggtaatcta cactaatttc gccgccacag tcatccatgc tgacatttac ttcgctggca
     6841 aaaacaccta cagtaaaaac accataagtt gtatcatagt atgatatgtg ttttcgtcct
     6901 ttttgaaaaa caaactgtga gtttaccgaa cctactctca ttagagttac aataccgtca
     6961 cagactttta tggtagttgt cgtcccatcc atacctgtaa cttcgctctc attataagta
     7021 acatagt
//
LOCUS       DAOSDD010000226         7023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3918_length_7023_cov_4.617968, whole genome shotgun sequence.
ACCESSION   DAOSDD010000226 DAOSDD010000000
VERSION     DAOSDD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_7023_cov_4.617968"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..170
                     /locus_tag="PK733_15820"
     tRNA            95..170
                     /locus_tag="PK733_15820"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Asn,seq:gtt)
     gene            complement(208..1512)
                     /locus_tag="PK733_15825"
     CDS             complement(208..1512)
                     /locus_tag="PK733_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOJ12040.1"
                     /translation="MRQNIFKGLIAGIVLVLIIASSVGAFNYFKNKNHALPKNNLDNA
                     GENNISGSNNNKSDKSSEIAHDSTGNKSDSGSKEEIPSTDENEMDNINTPYAPKIKKP
                     NAVKALYLTGWTAGESARLNKFIALSQQSEINSLVVDIKDDDGLVGYETNIPAVKEIN
                     GWTRKYNVDKVINTLHENDIYVIGRLVCFKDPVLSSKRADLAVKHASGGLWKDNNSKT
                     WLDPYNRDSWTYLIDIAKEALDKGFDEIQFDYIRFPNDGNKKAMVFNSGDIKKYEIIN
                     EFLSFAKKELPGAIISADVFGIICESPEDTEDIGQYLELVGKDIDSISPMAYPSHYAF
                     GQIVNNVKFAKPDLEPYAVVYNTLLKAKNRISSVENYKSNIRPYLQGFTAPWLRSGNY
                     QSYGAKQIREQIKAVYDAGFTEWIIWDPSNKYPADAFDGKVN"
     gene            1921..4227
                     /locus_tag="PK733_15830"
     CDS             1921..4227
                     /locus_tag="PK733_15830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOJ12041.1"
                     /translation="MNLTENAIKVLEKRYLAKDENGKLVENPEEMFRRVAKAITEADA
                     AYVDSAKLKQIEDEFFNLMTSLEFLPNSPTLMNAGRPLGQLSACFVLPVEDTMEDIFE
                     SIKNAALIHKSGGGTGFSFSRLRPKGASVKSTGGVASGPVSFMKVFNAATEAVKQGGT
                     RRGANMGILRVDHPDIREFITCKQDNKDITNFNISVGITEDFMRAVENNQQYDIIDPK
                     TGKPCGKENAREIYSIIIDNAWNNGEPGIIFLDRINKYNATPELGEIESTNPCGEQPL
                     LPYEACNLGSINLSIMVRKAEQGYDVDFNRLERVVKSAVHFLDNVIDVNKYPLKEIDE
                     MTRGTRKIGLGVMGWADLLCKLNIPYNSKAAMELAERVMRFIQEESKKASQVIAEVKG
                     VFPYYDKSIFKEKGIKMRNATTTTIAPTGTLSIIAGVSSGIEPLFAISYIRNVMDNDE
                     LLEVNPIFKDLAVKKGFYSNNLMKNVAKKGSIKGFTEIPAEVQSVFVTSHDIDPEWHV
                     EMQAAFQKYTDNAVSKTVNLRNEATRDDVKTVFEYAWKTGCKGVTIYRDGSRDLQVLN
                     IGSVKGKEKDNSAADQPNLAATQTNPAAGRPVPTTTQVAPRVRPDITTGFTEKVRIGC
                     GNLYITVNYDEQGICEVFTNLGRGGGCPSQSEATSRLISIALRSGMDVKSLVEQLKGI
                     RCATTIRQKGLKVMSCPDAIGKVIEKVMKLQSNGNGKEEELSVLAEIEDHDIENELMI
                     HNCPECGRKVEHEGGCVVCRHCGYSKCG"
     gene            4398..5357
                     /locus_tag="PK733_15835"
     CDS             4398..5357
                     /locus_tag="PK733_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ12042.1"
                     /translation="MTEKYINEFAAGEIVQNFFLIKTAECRVSNNSKKYLDITLVDKT
                     GEINAKLWDCNDSDEGMYIRNTLVKIRGNIVEWQGNMQLKIDKIRLVKKDDGLNIEDY
                     VPVAPLQAEYLYNELIGFVDRIQNEDIKKIVSYIIEMKKDKLMIYPAAMKNHHAIRSG
                     LLYHIVSMLQVGDRITDIYTSVNRDLLFAGIMLHDIAKLDEMDANELGLVSDYTLEGQ
                     LLGHITQGIVLIDNVTNLLGTDSETALLLKHMILTHHYEPEYGSPKKPLIPEAELLHY
                     IDMIDARMFDMEKALCNVAPSQFSEKIWTLDNRKLYKSKLKSV"
     gene            5416..6327
                     /locus_tag="PK733_15840"
     CDS             5416..6327
                     /locus_tag="PK733_15840"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOJ12043.1"
                     /translation="MASNIVVNQVAILFLIMVTGFAAKKCGIINLETRKKLSEMLVRI
                     TLPAMIIYSYTQQFSSELLQKGWIMLVYSFTIHIFSIILANFIYYKYPKDMKNVLKFI
                     TIYSNCGYMGLPILEALFGKTGIFYASMYITVFNIFTWTNGVMIFTGEKDIKSFKKAL
                     VNPGIIAVFIGIIIFIFSIKLPLPVIKTFEILGTMTTPLSMLIIGSLIADVKFKELFS
                     GAALYYGAAVRLIVLPLITLGVIKLTGITDTVLIGACVTTAAMPAAANTAVFAERYNG
                     DSAFASRTVAFTTILSMITIPAILSLI"
     gene            6561..>7023
                     /locus_tag="PK733_15845"
     CDS             6561..>7023
                     /locus_tag="PK733_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12044.1"
                     /translation="MQFYTFATYFIIGTILVLFSLLIFSGTTGKKREFEIDDAILGPE
                     ELKEHAIGIARNHFVKRSSRTANWLILRMNKNYAVILEAYKTLNADILHDFPVPPAAE
                     WLLDNFYVIEEEVKDIRYNLPGKHLNLLPVLKSGYLKGYPRIFAIALEIVSH"
BASE COUNT         2401 a         1103 c         1347 g         2172 t
ORIGIN      
        1 gccgatgata cttggatggt aacgtcctgg gaaagtaggt ccctgccgga tttatttgga
       61 tagaacaggt gaaaacctgt tttatccttt atattcctca atagctcagt cggtagagca
      121 tgcggctgtt aaccgcaggg tcgtaggttc gagtcctact tgaggagcca aacaaaaagg
      181 acagatattt atctgtcctt tttgttatta attaaccttg ccatcaaaag catctgcagg
      241 gtatttgtta cttggatccc aaataatcca ctccgtaaaa ccagcatcat atacagcctt
      301 tatttgctct cttatctgtt ttgcaccata tgattgataa tttccactgc gaagccatgg
      361 agctgtaaaa ccttgcaaat atggtctaat atttgactta taattttcaa ctgatgaaat
      421 tctgttcttt gcttttagca aagtattata aacaacagca taaggttcca aatccggttt
      481 tgcgaactta acattattga ctatctgccc aaaagcataa tgagatgggt atgccatagg
      541 agaaatagaa tcaatgtcct taccaacgag ttccagatat tgaccaatat cttccgtatc
      601 ctccggactt tcacagataa taccaaacac atcagcagaa attatagctc ctgggagctc
      661 cttttttgcg aaagataaaa attcgtttat gatttcatac ttttttatat ctcctgagtt
      721 aaaaaccatt gcttttttat taccatcatt gggaaaacgg atataatcga actgtatttc
      781 atcaaagcct ttatccagag cttctttagc tatatctatt aaataagtcc atgaatctct
      841 attatatggg tccagccatg ttttactgtt gttatccttc catagaccac ctgatgcatg
      901 cttaactgca agatcagctc ttttagaaga aaggacagga tctttaaagc ataccagtct
      961 tcctataaca taaatgtcat tttcatgtaa agtattaata accttatcaa cattatattt
     1021 tcttgtccag ccgtttatct cctttacagc agggatattt gtttcataac ctacgagtcc
     1081 gtcatcatct tttatatcaa ccacaagaga atttatttca ctttgttgag atagtgcaat
     1141 aaatttattt aaccttgctg attcacctgc agtccaaccg gttaaatata aggctttaac
     1201 tgcatttggt ttcttgattt taggtgcata tggcgtatta atattatcca tttcattttc
     1261 atctgtagaa ggtatttcct ctttagaacc ggaatcgctt ttattaccgg tactgtcatg
     1321 cgctatttca gagcttttgt cagatttatt attattactt ccacttatgt tgttttcacc
     1381 cgcattatcc aaattattct taggtaatgc atgattctta tttttaaaat aattgaatgc
     1441 acccacagaa gaagctatga ttaatactaa tactatacct gcaattaaac ctttaaaaat
     1501 attctgtctc attatacatt actacctcca atttaatctt tagtattatt cccctaatag
     1561 aaggataatt ataaaataga cataaagctg gactttggtg tagtctgttt atgtttattt
     1621 tcttcaaaag ttattaaaaa cacattttta attatacttt atataattag acttgccaat
     1681 acgttaaaaa tacaataaaa atgatagata tgaatattga cactctgcat taaaatgata
     1741 caatatattg tgttgataaa taactataca caaagtgtag aaattatatt taatgtagca
     1801 atctataatt aatgtaaaaa tctataaaaa atgtagtata tcctatacat tacaacaaaa
     1861 tttgttaatt tattagttat taatctattc atagatatgt aaataagggg gtagcaaaat
     1921 atgaacctga ctgaaaatgc tataaaggta cttgagaaaa gatatcttgc caaagatgaa
     1981 aatggaaaac ttgtggaaaa tccggaagaa atgtttagaa gagttgcaaa agctataact
     2041 gaggcagatg cagcttatgt agattcagca aaattaaagc aaatagaaga tgagtttttc
     2101 aacttgatga ccagcttgga gtttttaccc aattcaccta cacttatgaa tgcaggcagg
     2161 ccattaggtc agctcagtgc atgttttgta cttccagttg aagatacaat ggaagatata
     2221 tttgaatcaa taaaaaatgc agctttaata cataaaagcg gcggtggtac gggctttagt
     2281 ttttcaagac tcagacccaa gggagcatca gttaaatcta caggaggagt tgcaagcggt
     2341 cctgttagtt ttatgaaggt ctttaatgca gctacggaag ctgtaaaaca gggaggcaca
     2401 cggagaggag ccaatatggg cattctgcgt gtggatcatc cggatattcg tgaattcatt
     2461 acatgcaaac aggataataa agatattacc aattttaata taagcgttgg catcactgaa
     2521 gattttatgc gtgctgttga gaataatcaa caatacgaca taattgatcc aaaaacaggt
     2581 aaaccctgtg gtaaagaaaa tgccagagaa atctacagta taattataga caatgcctgg
     2641 aataatggag aacctgggat catattttta gacaggataa ataagtataa tgccacacct
     2701 gaactgggcg aaattgagtc tacaaatcca tgcggggagc aaccgctgct tccttatgaa
     2761 gcatgcaacc ttggctcaat taatttaagc attatggtta gaaaagctga acaagggtat
     2821 gatgttgatt ttaatagatt agaaagagta gttaaatcag cagtgcattt tctggataat
     2881 gtaattgatg ttaataagta tcctcttaaa gaaattgatg aaatgaccag aggtacaaga
     2941 aaaatcgggc ttggagtcat gggttgggct gacttgctct gcaaactcaa tataccttac
     3001 aactcaaagg cagcaatgga gcttgctgaa agagtaatga gatttataca ggaagagtca
     3061 aaaaaggctt ctcaggttat tgctgaagta aagggggtat tcccatatta tgacaaaagt
     3121 atatttaagg aaaaaggaat aaaaatgagg aatgctacta ctacaactat tgctcctaca
     3181 ggtacattga gcataatagc cggagtatcc agtggcattg agcctttgtt tgctatatca
     3241 tatattagga atgtaatgga taatgatgaa cttctggaag ttaaccccat atttaaagat
     3301 ttagcagtaa aaaagggttt ttattcaaat aacttaatga agaatgtagc taaaaagggt
     3361 tcaattaaag gctttactga gatacctgca gaagttcaaa gcgtttttgt tacatcacat
     3421 gatattgacc cggagtggca tgtagaaatg caggccgcat ttcaaaaata tacggataat
     3481 gctgtttcaa agactgttaa ccttcgcaat gaggcaacaa gggatgatgt aaaaacagtg
     3541 tttgaatatg cctggaaaac cgggtgtaaa ggcgtaacaa tttacaggga cggaagcagg
     3601 gatttgcaag tattaaatat cggaagtgta aaaggtaaag aaaaggataa ctctgcagct
     3661 gatcagccta acctcgcagc aactcaaact aatccagcag caggccggcc tgttcctaca
     3721 acaactcagg ttgccccaag agtaagacct gatattacaa caggatttac tgaaaaggtt
     3781 agaatcggct gtggtaatct atatataaca gtaaactatg acgaacaggg tatatgtgaa
     3841 gtatttacaa accttggccg cggtggtgga tgtccttcgc aatcggaagc taccagccgt
     3901 cttatatcta tagctttaag gtcggggatg gatgtaaaat ctcttgtaga acaacttaag
     3961 ggaataagat gcgctacaac tataagacaa aaaggcttaa aagtaatgtc atgtcctgat
     4021 gcaataggaa aggtaataga aaaagtaatg aagctgcaaa gcaatggaaa tggtaaagaa
     4081 gaagaattaa gcgtactggc tgagatagaa gaccatgata ttgaaaatga attaatgatt
     4141 cataattgcc ctgagtgtgg aaggaaagtt gagcatgagg gcggatgtgt tgtttgcagg
     4201 cattgcggtt attcaaagtg tggataatcg ctcagtagtg taggtgttgt agagcctttt
     4261 aatccgagcg aatgatatgc aaaataagtg tttgaacaaa attgataaat gcacccatgg
     4321 gttggtattt ggttgacatc atttaaggta ataggtatac ttgattatac tggaaaaaat
     4381 tcagatgagg tggatatatg actgaaaagt acataaatga atttgctgct ggggaaattg
     4441 ttcagaattt cttcttaata aaaactgccg aatgtagggt ttcaaataac agcaaaaagt
     4501 atcttgatat taccctggtt gataaaacag gggagataaa cgcaaagctt tgggactgta
     4561 atgatagtga tgaaggcatg tatatcagga atacactggt taaaataaga ggaaacattg
     4621 ttgaatggca gggtaatatg cagttgaaga ttgacaaaat aagacttgta aaaaaagatg
     4681 atggtttaaa tatagaggat tatgtcccgg tagcgccatt gcaggcagag tatctttata
     4741 atgagctaat cggatttgtt gatagaattc aaaatgaaga tataaagaaa attgtcagtt
     4801 acataattga aatgaaaaaa gacaaactta tgatttatcc ggcagcaatg aaaaatcatc
     4861 atgcaatacg ttcaggcctg ctttatcata tagtaagcat gcttcaggtt ggagacagga
     4921 taactgatat atatacttct gtaaacagag atctgctttt tgcgggaatt atgctgcatg
     4981 atattgcaaa gcttgatgaa atggatgcaa atgaactggg acttgtttct gattatactc
     5041 ttgaaggcca gcttctaggt catattaccc agggtattgt attgatcgac aatgtgacca
     5101 atttgcttgg aacagatagt gaaactgcat tgctgcttaa gcacatgata ctgacacatc
     5161 attatgaacc tgagtatgga agtcccaaaa agccactgat accggaagca gaactgctcc
     5221 attatattga catgatagat gcaagaatgt ttgatatgga aaaagcccta tgcaatgtag
     5281 caccatcaca attttccgaa aaaatctgga cccttgataa cagaaaactt tataaatcca
     5341 aactgaaaag cgtttaactg aaaggcgttt aactgaaaag catttaagga aaaagcattt
     5401 agggggacaa aaaatttggc aagcaatatt gttgtaaatc aggtagcgat acttttttta
     5461 attatggtaa cgggttttgc agcgaagaaa tgtggaatca tcaatttgga aacgagaaaa
     5521 aaattatcgg aaatgcttgt aaggataact ttaccagcca tgataatata ttcatatacc
     5581 cagcaatttt caagcgaact cttgcagaaa ggctggataa tgcttgttta ttcctttaca
     5641 atacatatct tttccataat attagctaat tttatatatt acaaatatcc taaggatatg
     5701 aaaaatgtat taaagtttat aactatatat tcaaattgcg gttatatggg gttacccatt
     5761 cttgaggctt tgttcgggaa aacagggatt ttttacgctt ccatgtatat tacagtattc
     5821 aatattttta cgtggaccaa tggagttatg atatttactg gtgagaagga cataaaatca
     5881 tttaaaaaag cgctggttaa tccaggaata attgcagttt ttattgggat tataattttc
     5941 attttttcaa taaaactacc attgccggtt atcaaaacat ttgaaatact tggcaccatg
     6001 acaacacctc tatctatgct tattataggg tcgctgatag cggatgttaa attcaaggaa
     6061 ctgttttcgg gagcagctct gtattatggt gctgcagtac gattaatagt gttacccctt
     6121 attactttag gtgttataaa gctgacaggg ataactgaca ctgtattaat tggtgcctgt
     6181 gttacaactg ctgctatgcc cgcagctgca aatactgcag tttttgctga aagatataac
     6241 ggagattcgg catttgcttc aagaacagtt gcttttacaa ccatattatc tatgatcaca
     6301 atacctgcta tactttcgct gatttaaaaa taataaaata tagcatcaaa accatacaca
     6361 gtcttttgaa ttcctggtaa ggttagaaag aatatttatt ttcatagaaa tattgtagta
     6421 ttacttaata aatcttaaaa tatcaccatt tttgagtttg tttgccacac aaaaatatgg
     6481 cagtaatgga ttattaataa ttacatattt gggcaatact aatactgcaa aaatacatca
     6541 gggaatggtg aaataagatt atgcagtttt atacatttgc tacatatttt ataattggaa
     6601 ctatactagt acttttttca ttgttaatct tttctggtac aacaggcaaa aaacgtgaat
     6661 ttgaaataga tgatgcaata cttggaccag aagagttaaa ggaacatgca atagggatag
     6721 caaggaatca ttttgtaaaa aggagttcac gaacagcaaa ttggcttata ttaagaatga
     6781 ataaaaacta tgctgttatt cttgaagctt ataaaacatt aaatgccgat atccttcatg
     6841 actttcccgt gcctcctgca gcagaatggc ttcttgataa tttttatgtt attgaagagg
     6901 aagttaaaga tataagatat aatttgcccg gaaaacactt gaatctgctt ccagtcttga
     6961 aaagtggata tttaaaaggt taccccagaa tatttgctat tgcattggaa attgtttctc
     7021 act
//
LOCUS       DAOSDD010000227         6979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3948_length_6979_cov_5.026430, whole genome shotgun sequence.
ACCESSION   DAOSDD010000227 DAOSDD010000000
VERSION     DAOSDD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_6979_cov_5.026430"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1033
                     /gene="argB"
                     /locus_tag="PK733_15850"
     CDS             164..1033
                     /gene="argB"
                     /locus_tag="PK733_15850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ12045.1"
                     /translation="METIFKRAEILIEALPYIQKYYGKTVVIKYGGNAMINDELKNSV
                     MQDIILLKYVGMNPVVVHGGGPDLTHALERMNIKSEFINGLRVTSESVIEVAQMVFIG
                     KTNKEIVSLLNSKGGKAIGLSGIDGNLIECEKLKMQVDGKETDLGYVGNITNINSKLI
                     ELLAADEYIPVIAPIGIGKDGQSYNINADTVAAEVAVALKAEKLILLTDVQGVKTSGN
                     KEEIIPALTIEQAYNWVDKKIINGGMIPKVLGCIDALNKGVGRTHIIDGRVPHCILLE
                     IFTDEGIGTMIIK"
     gene            1288..2484
                     /locus_tag="PK733_15855"
     CDS             1288..2484
                     /locus_tag="PK733_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ12046.1"
                     /translation="MKLKEIIDLDKKYYMNTFGDRTPVCFTHGKGINLWDLDGNKYYD
                     FMAGIAVSALGHSHPKLVKAIQEQAGKFIHCSSLYYIEQQAKLAQILVENSCADRVFF
                     ANSGAEANEGAIKLARIYFKKKGMENKYEIITLEKSFHGRTLATIAATGQDKYQKPFS
                     PLTPRFSSVPINDIDALEKAVNNDTCAIMIEPIQGESGVNLTTIQYMHDVRKLCDEKG
                     ILLIFDEVQCGLGRTGKLFGYQHYGVEPDIFTLAKALGGGVPTGALLAKEFVAQAFGK
                     GDHGSTFGGNPLACAAGIAVMDALLNDGLVENSEKMGNYFISKLNELSKKYSFIRNVR
                     GKGLMIAVEFSIDKAEEIKNKCFEKHYLLGSVGKNILRILPPLIVSQQDIDEMVKMLD
                     EVLAEF"
     gene            2655..3572
                     /gene="argF"
                     /locus_tag="PK733_15860"
     CDS             2655..3572
                     /gene="argF"
                     /locus_tag="PK733_15860"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOJ12047.1"
                     /translation="MKHLISIHDITADEINEIFELSDKLKKALKEGKDHSYLKGKTLG
                     MIFTKSSTRTRVSFEVGMYQLGGYSLFLSSDDIQLGRGETIYDTAQVLSRYLNGILIR
                     TFSHDDVMDLARYGSIPVINGLTDLMHPCQVLADLFTIYEKKGDLKGLKLAYIGDGNN
                     MTHSLLHGCAKVGMNISVATPPEFKCNDSIVNEAMEDAKISGADILIANDPAIAIRDA
                     DVVYTDTWVSMGQESEKEQRLKVFKPYQVNKDMFALAKSDAIFLHCLPAYRSYEVTSD
                     VIDGPNSVIFDEAENRLHVQKAIMVKLMA"
     gene            3708..4181
                     /locus_tag="PK733_15865"
     CDS             3708..4181
                     /locus_tag="PK733_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ12048.1"
                     /translation="MSYSLLIKKAEISDAQAIHAIINEAFNKYIKDAGICGRMEALEE
                     TPEDIIQDMNTKEVFIALADNIPVGTIRVEIKADNTAFISRFGVLPQYQKNGIGRSLI
                     TFLDNFLVSKGVNKVYLYTASRHRDLVRFYYGMGFYIDSTTKDKGYIRAMMVKEY"
     gene            complement(5051..6436)
                     /locus_tag="PK733_15870"
     CDS             complement(5051..6436)
                     /locus_tag="PK733_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOJ12049.1"
                     /translation="MQPFDEIKQYSKTVCEQIRWKKAHNIISEEIENHLCDQRDAYIS
                     QGEDEKSATQKAILQMGDAVSIGMELDKTHKPKPQWTMIILTAALMLIGMLSNYFIAS
                     SEYSSDRFSIAPYIIALTVFLVCYFLDFSILGKCAKYCYFTVLAVSVTGLLFGNQING
                     RVWFPPGRLSISLSYLSLIFPLAYSLLIYAMRHKGYKGIMLCGVGYVPYAIILLLVPS
                     VTGFALFTISVLVILCMSVVKGWFGIDKKRGLLLVLIPAFIAIIAAILNIIQNTYFLN
                     RLSTAVNPYSDRDGSGYIHCLVRDLLANSQFAGKGTVPAQFGNNVPIIPALDTDYILT
                     ALTHNFGWVAFFGISVVIIVFSIFGLYYVTKQKSVLGTIISLSIMLTFIMQAIIYIMN
                     NLGYGILSALSLPLVSYGNVALIINSALIGFMLSVFRTGDILKDSIKSPVKINSIFSY
                     EDGKLIINFKG"
     gene            complement(6423..6770)
                     /locus_tag="PK733_15875"
     CDS             complement(6423..6770)
                     /locus_tag="PK733_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ12050.1"
                     /translation="MSIDKSLLTGSTTTLILKLLEEKDMYGYQMIETLAQKSDDTFNL
                     KAGTLYPILHNLENNGIVESYEEEADNGRTRKYYHLTSKGKKLLQEKHQEWITYYSAV
                     NRILNGGVSYATV"
BASE COUNT         2400 a         1110 c         1340 g         2129 t
ORIGIN      
        1 tgagaagtgg acacatagta aaaagtatga ggtataaagc atgagcgtta gatgttaggt
       61 ataaagtgta agatgtaaaa tattggattc gaggttaggg tttgaagttt ggggtttggg
      121 gtttaacatg taaaatgcaa taaatgggat acggaggaat aatatggaaa caatctttaa
      181 acgggcagaa atattaatag aagcattgcc ctatatacaa aaatattacg ggaaaactgt
      241 ggttataaaa tacggcggaa acgcaatgat aaatgatgaa cttaaaaact ctgtaatgca
      301 ggatatcatt ctgcttaaat atgtaggcat gaatcctgtt gtggtgcatg ggggcggtcc
      361 tgaccttact catgctctgg aaaggatgaa tattaaaagt gagtttatta atggtctcag
      421 ggttacaagt gaatctgtta ttgaggtcgc tcaaatggta tttattggta aaaccaacaa
      481 ggaaattgtt tcgcttctta attcaaaagg tggaaaagct ataggtctca gtggaattga
      541 cgggaatctt attgaatgtg aaaaattaaa gatgcaggta gacggaaaag aaactgattt
      601 aggctatgta ggcaatatta ccaatattaa ctcaaaactg atagagcttc ttgctgctga
      661 tgaatacata cctgttattg cacctatagg tataggaaaa gatggtcaga gctataatat
      721 taatgcagat acggtggctg cagaagtggc tgtggctttg aaagctgaaa aacttatctt
      781 gcttacggat gttcagggag taaaaacttc aggaaataaa gaggaaataa ttcctgcatt
      841 aacaatagag caagcctata attgggttga taaaaaaata attaacggcg gcatgattcc
      901 caaagtactt gggtgtattg atgctcttaa taaaggtgtt ggcaggacac atataataga
      961 tggaagagtt cctcattgta ttttgcttga aatatttaca gatgaaggta ttggcactat
     1021 gattataaaa taggctggca gcaaatgtaa aacagactag caacgaacac aaaatagaat
     1081 gacggtaaat gtaaaataaa ctgatagcaa atgtagaaaa agtatgcgat aaaaagcaag
     1141 ctcactctgc tcggaagtta atttgtctta caatttacaa tttacaattt gaactttgaa
     1201 atttactaga atacaaaaaa tattgttgca tatttattca ctttaatgta taattattaa
     1261 tataatcttt atgggagatg tgtttaaatg aagcttaaag aaataatcga tttagataag
     1321 aaatattata tgaatacctt cggggacaga accccggtat gtttcacaca cggcaaaggt
     1381 ataaacctct gggatcttga tggaaacaaa tactatgatt tcatggcggg catagcggta
     1441 agcgccctgg ggcattctca tccgaaactc gtaaaagcta ttcaggaaca agccgggaag
     1501 tttatccatt gttcaagcct ttattacata gaacagcagg caaagctggc tcagattctg
     1561 gtagaaaact cctgtgcaga cagagtgttc tttgccaata gcggtgcaga agcaaatgaa
     1621 ggagcaatta aacttgccag aatatatttc aagaaaaagg gcatggaaaa taagtacgaa
     1681 attataactc tggagaaatc ctttcatgga aggactcttg caactatagc agccacaggt
     1741 caggataaat atcagaaacc attctccccc cttacgccca ggttttcaag tgtacccata
     1801 aatgatattg atgctctgga gaaagcagtt aataatgata cctgcgccat tatgatagaa
     1861 ccaattcaag gtgagagcgg tgttaatctg acaactattc aatatatgca tgatgtcagg
     1921 aagctttgcg atgaaaaagg catcttgttg atatttgatg aagttcaatg cggacttggg
     1981 agaacaggaa agctctttgg atatcagcat tatggcgttg aacctgacat atttaccctg
     2041 gcaaaagcac ttggaggagg cgttcctaca ggagcactgc tggccaaaga gtttgtggca
     2101 caggctttcg gcaaaggaga tcatggttcc acttttggcg gaaatcctct ggcatgcgca
     2161 gcaggaattg cagtaatgga tgcgctgttg aacgatggtc ttgttgaaaa ctcagaaaaa
     2221 atgggcaatt actttataag caagctaaat gagttgtcga agaagtacag ttttatcagg
     2281 aatgtacgtg gtaaaggcct tatgattgct gtagaatttt caatagataa agcagaagaa
     2341 ataaagaaca agtgctttga aaagcactat ttattaggaa gtgttgggaa aaatattcta
     2401 aggatactgc caccattaat agtttcgcag caagatattg atgaaatggt gaaaatgctg
     2461 gatgaagtgc ttgctgaatt ttgataaact gatggactga taagctgata gctgattgac
     2521 cgattaaatt ggattaattg gttaatttag atgaacttag caaactgtat atgcatttgt
     2581 tcctcgcagc aaatgcatat acaggtgcat atattagaag ttaagaaata ttgaagcaat
     2641 gggggttgtg cttaatgaaa catttgataa gcattcatga tattacagct gatgaaatta
     2701 atgaaatatt tgaattatca gataaattga agaaagcctt gaaagaaggc aaagatcaca
     2761 gctatttaaa ggggaaaacc ctggggatga tctttacaaa atcttcaacc agaacccgag
     2821 tttcctttga agttgggatg tatcaacttg gagggtattc ccttttcctg agttctgatg
     2881 atattcaact cggacgtggt gaaacaatct acgacactgc tcaggtttta tccagatatt
     2941 taaatggaat actgatcaga actttttcac atgatgatgt aatggatctg gccaggtatg
     3001 gcagcatacc ggtaataaat ggtttgactg atttaatgca cccatgccag gtccttgcag
     3061 atttatttac tatatatgag aaaaagggtg atttaaaagg actaaaactt gcgtatatcg
     3121 gtgatgggaa taatatgact cattctctgc ttcacggctg cgctaaagtc ggtatgaata
     3181 tatctgtagc tacacctccg gaatttaaat gtaatgattc tatagttaat gaagctatgg
     3241 aggatgcaaa aatatcagga gctgatatcc taatagctaa tgatcctgct atagcaataa
     3301 gagatgctga tgttgtatac acagatacct gggtaagcat ggggcaggaa agcgaaaaag
     3361 aacagagact taaagtattc aaaccctacc aggtaaataa ggatatgttc gcacttgcta
     3421 aaagtgatgc tatattcctt cattgtctgc ctgcttatag gagttatgaa gttacgagtg
     3481 atgttattga tggccctaat tcagtaatat ttgatgaagc agaaaaccgt ctacatgtac
     3541 aaaaagctat tatggtaaag ttaatggcgt aaaagctttt gacgtaaaaa aatggtcaga
     3601 aaatttttct acaaccgaat aattttctac tgatgcgtta taacactatg aaattgtaaa
     3661 gtaaacacta tgaagttgtg aagttttgga taagtggtgg tcgaaatatg agttattctc
     3721 ttttaattaa aaaagcagaa ataagtgatg cgcaagcaat acatgctata attaacgaag
     3781 cttttaataa atatattaag gatgccggaa tatgcggaag aatggaggct cttgaagaaa
     3841 ctcctgagga tataatccag gatatgaata ctaaggaagt tttcatagct cttgctgata
     3901 acattccggt tggcactata agggttgaga taaaagccga taacacagct tttatttcac
     3961 gctttggggt attgccccaa tatcaaaaaa atggtatagg cagatcattg ataactttcc
     4021 tggataattt tcttgtgtca aaaggagtta ataaagttta cctttataca gcatcaaggc
     4081 accgggatct tgttcgcttt tattacggta tgggctttta tatagactcc accacaaaag
     4141 ataaagggta tataagagct atgatggtta aggagtattg atgaaaggga aattatcccc
     4201 tccatttttg cccatccatc aaccatgcgg gtttgcaggg atgaggttgg aaagagttta
     4261 tttgataatc acatagatag gactaaggaa ttaaggggta agggttaagg tttttgataa
     4321 aatcaggtta agtcgttcca aaatgaaaat aatgttgttc caaaaccctg ttttctgcaa
     4381 ctttggaaag actctatttg caaaaaggaa agactttatt tacttctctc tacccagtca
     4441 tgacctcgtt gggaaaataa agttgttcct gagtaaaata atgtctttcg ggaaaagttt
     4501 gcttggttta ttcaaaatat ttaagtcctt tattcacata tttagaatga cgcgtcatat
     4561 ggataaacag gctattattc ctttttgctt aatatcccca atcaacaatc tcccattttt
     4621 tagacttacc ccctcttttt aatatccaca tccaattagt ataatatgaa tttggattaa
     4681 atccttcctt tatagcatgt ttgtttgtct caaaattgga aagtaacaca attttgtttt
     4741 cagtttcagt ttcaatatat acatcagatt tttcttcatc ataccataat ttagttaaat
     4801 tacacccttt aaagtctcta aactttagtt taacaagaga tatggcttct ttaatttccg
     4861 tttctgaaaa cttatcagaa tgtccaatag ttacttaaac attttttact ttacccgaat
     4921 tacaagcaac taaaataata caaaaagaaa tacagataac taaaagtaat ggtttactca
     4981 ctgtgaacgc ctccataaaa tataaataat atgcattacc ttagttacac attatgctca
     5041 taaaagttag ttatccttta aaattgatta tcaatttccc atcttcgtat gaaaatattg
     5101 aattgatttt tacaggagat tttatactat cttttagaat atcccctgtt ctgaaaactg
     5161 aaagcataaa tcctattaaa gcagaattta ttattagtgc tacattccca taagagacca
     5221 gcggtaatga caatgctgac aatattccat acccaaggtt attcataata taaatgattg
     5281 cctgcataat aaaggtaagc attattgata acgatattat agttcccaaa acactttttt
     5341 gcttagtaac ataatataac ccgaaaatag aaaatactat aataactact gatattccaa
     5401 aaaatgcaac ccagccaaaa ttatgcgtca atgcagtaag aatataatct gtgtctaaag
     5461 caggtattat gggaacatta ttgccaaatt gagcaggtac agttcccttg cccgcaaatt
     5521 gagagtttgc taataaatct cgcacaagac aatgaatgta accagaacca tcgcggtcgg
     5581 aatatgggtt gactgcagta ctaagtctgt ttaaaaaata ggtgttttgt atgatattga
     5641 gaatggcagc aataatcgca ataaatgcag gtatcagcac taataacaaa ccacgctttt
     5701 tatcgatgcc aaaccaccct tttacaacag acatacaaag gataacaagt accgatattg
     5761 taaataaagc aaaaccagta actgacggta caagcaataa tataattgca tacggaacat
     5821 aacctacacc acataacata atacctttat atcccttgtg tcgcatagca taaataagta
     5881 atgaataagc aagggggaat attaaggaaa gataggataa acttatagac aatctgcctg
     5941 gcgggaacca aactctgccg tttatttggt tgccaaacaa aagccctgta accgaaactg
     6001 ctaaaacggt aaaatagcaa tattttgcgc atttccccaa aatggaaaag tcaagaaaat
     6061 agcatactaa aaacacagtc aaagcaataa tatagggagc aatgctgaat ctgtccgagg
     6121 aatattcaga agaagcaata aaataatttg aaagcatgcc tattaacatt aacgcagcag
     6181 tcaggataat cattgtccac tggggttttg gcttatgagt tttatccaac tccataccga
     6241 ttgacactgc atcacccatc tgtaatattg ctttttgtgt tgctgatttt tcatcctcgc
     6301 cctgagaaat ataggcatcc ctttggtcgc aaagatgatt ttcgatttct tccgaaataa
     6361 tattatgagc ctttttccaa cgtatttgtt cgcatacagt cttgctatat tgttttattt
     6421 catcaaacgg ttgcataact tactcctccg ttcaaaattc tgttgaccgc agaataataa
     6481 gtaatccatt cttgatgttt ttcttgtaaa agttttttac ccttagaagt aagatgataa
     6541 tattttcttg ttctcccgtt atctgcttct tcttcgtaag attcaacaat tccgttattt
     6601 tcaagattgt gtaaaatggg ataaagtgta cctgctttca agttgaaagt atcatctgat
     6661 ttttgtgcaa gagtttcaat catttgatac ccatacatat ctttttcttc aagcaatttt
     6721 aaaatcagag ttgttgtact acctgtcaac aggctcttat ctatagacat atatagccct
     6781 cctttatata tagattatct acatatgaat tatatatcgg caatctatat atgtcaatag
     6841 aaatgtgcaa acttttaaag ttttcacatg agtaaaatat aaattatcct tactacacgt
     6901 tattctactg atacgtaata taagaagatt gtgacattaa ctccattttc tatttgtttc
     6961 ttttacatat attcgaaca
//
LOCUS       DAOSDD010000228         6915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_3997_length_6915_cov_4.376385, whole genome shotgun sequence.
ACCESSION   DAOSDD010000228 DAOSDD010000000
VERSION     DAOSDD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3997_length_6915_cov_4.376385"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1938)
                     /locus_tag="PK733_15880"
     CDS             complement(37..1938)
                     /locus_tag="PK733_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOJ12051.1"
                     /translation="MSISIDDNILIQVEKPARYTGNEWNMVKKNPGNMDIRFAFCFPD
                     LYEVGMSHLGMKILYHLLNERDDTYCERVFAPWTDMESKMREKSIPLFSLETKEPVKN
                     FDFVGFTLQYEMSYTNVINMLDLAGIPIRAKDRTDAHPFIIAGGPCAYNPEPLAEVID
                     FFVLGEGEEVINEILDAYKEWKQKRISSSQNMISSRIETEYTRESFLDIAAKIKGVYV
                     PSFYEVKYNYDGTVNKIQPKNSEYPAKIKKRIIKDYENVYFPEKIIVPYVNIVHDRIM
                     LELFRGCTRGCRFCQAGFVYRPVRERKPEKLIEIAEKLEAATGYEEISLASLSTSDYT
                     GLKDLTEGLLENMCEKGVSLSLPSLRIDSFSLELMEKAQKVRKSGLTFAPEAGTQRLR
                     DVINKGVTEEDLLNSAGIAFNGGWTGVKLYFMIGLPTETFEDIEGIVVLGNKVIDAYF
                     NVPKEKRGKGLNITISTSSFVPKPFTPFQWEAQDTIEGLREKQKYLKSLIKSKHITYN
                     WHDPELSFLEAVFARGDRKTGEVLIKAWEKGCKFDGWGEHFNYSKWLEAFEECGVDPH
                     FYANRKRSYDEVFPWDHIDIGVSKKFLINENKKAYEGQLTPNCRVDCSNCGAASFGGG
                     VCVERAPVR"
     gene            complement(2165..3046)
                     /locus_tag="PK733_15885"
     CDS             complement(2165..3046)
                     /locus_tag="PK733_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOJ12052.1"
                     /translation="MELLQLKYFYSAARYESITRAAQQYGIPQPAMSQTISRLEKELG
                     TQLFDRKSNRLTLNENGKLFLKHVEAVLDNLSDGIMALKEKNGDIKGEISLLVLENRN
                     MIIDWIVEFARLNPEVSFSICHNNIGDHSFNFDLCVSSRRMQPHLRKGIPLICEEIML
                     AVPYHHALAKNKSVTLNSLKDERFITLPSQYSLSQTVKEYCRAHGFEPKISIICDDPY
                     YIRKYVSLGMGVSFVPSFSWQGLWNENVIFLPIQGEKFTRTTYLIWDDRRYMTKAVQQ
                     FRDYIIEKASDYHIYQG"
     gene            3219..4001
                     /locus_tag="PK733_15890"
     CDS             3219..4001
                     /locus_tag="PK733_15890"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ12053.1"
                     /translation="MYEIKRILDITYSKVHPEDCKLDLYLPETKLPCPVLIYIHGGGL
                     EAGTRRNTSGWIEALVSEWGIAVVSLDYRMYPNAHFPDFIEDCAEGTAWVTSYGRTHS
                     CFNKYYIGGSSAGAYLTMMLYFAENFLLKHGLSPDEFDGYIFDAGQPTTHFNILRERR
                     IDTRAIRVDDAAPVFYVDKQIEEPEKKPPVLVLVAENDIPNRLEQNEMFIGTMINFGF
                     IPSRITKKYMAGFSHCGYVNARDSEGKSIFANILANFIDRGN"
     gene            4021..4908
                     /locus_tag="PK733_15895"
     CDS             4021..4908
                     /locus_tag="PK733_15895"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ12054.1"
                     /translation="MKKGLIYIILTAILFTTLEPVSKLISTEISPVRITFIRFFIGSL
                     MLLPFSIAQIKKENIKINTKDIYTMSFLGILCICVSMILLQYAVLKAESPALIAIIFS
                     SNSVFTIIFAAFILKDEITWAKLCAIALCLAGLLVSTDFKLSENMLSVVLAILAALAF
                     SLYTVLSKKFMTKVSGIIQTGFSFFIGSLVLLIALLIGGTDIFGGITSSNIWELLYLG
                     VAVTGIGYWSYFRAMDKASAMSASFVFFIKPALTPLATFVINGIVPDIKVFISLVLVI
                     TGSYMATVKTQPKNNLTKA"
     gene            complement(4981..5436)
                     /locus_tag="PK733_15900"
     CDS             complement(4981..5436)
                     /locus_tag="PK733_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12055.1"
                     /translation="MLEDKQKDLLILKKNIPKAIRNEYILTLEELKESKDIIKAEVNE
                     LLTQINTVDENLKQSKIALDEYSKLDENIVSFVDRYNKADFLTKKGMLNEIIEKVVIY
                     RDSVEIEYKVTINVFKENSVDNTGCEIDSNGLPSELHPNIFCTTNSYIF"
     gene            complement(6011..6175)
                     /locus_tag="PK733_15905"
     CDS             complement(6011..6175)
                     /locus_tag="PK733_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12056.1"
                     /translation="MDTKKEKIIKLLDQFKEAIIEDKYSIYKYYIADLFDELAQLKSD
                     QSEAIRNRDK"
     gene            complement(6266..6514)
                     /locus_tag="PK733_15910"
     CDS             complement(6266..6514)
                     /locus_tag="PK733_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12057.1"
                     /translation="MEIGKIYTLKEIKKFLSKKKNTLVISDVTFLIDEIYDKYEYKIV
                     NYLEDKYAVECEFMNLMSKTKSPIKVPILELKLISSKY"
BASE COUNT         2140 a         1293 c          994 g         2488 t
ORIGIN      
        1 ctttaccaga tgggattcgc acccactata tattaatcac cgaacaggcg cacgctcaac
       61 gcacacacct cccccaaaag atgctgctcc gcagttactg caatctactc tgcaatttgg
      121 tgttaattgg ccctcatatg cttttttgtt ctcattaata agaaatttct ttgaaacccc
      181 aatatcaata tgatcccatg gaaatacctc atcatagctg cgcttcctgt ttgcataaaa
      241 gtgaggatcc accccgcatt cttcaaaggc ttccaaccat ttggagtagt taaagtgttc
      301 accccaacca tcaaacttgc atcctttctc ccatgccttt atcaatactt cacctgtttt
      361 tctgtcacct cgtgcaaata ctgcttccag aaaacttaat tcgggatcgt gccagttata
      421 tgtaatatgc ttgcttttaa tcaggctttt aagatacttt tgcttttctc gtaaaccctc
      481 tattgtatcc tgggcttccc attgaaaagg tgtaaatggc ttagggacaa aggaagatgt
      541 gcttatagta atgttcaacc cttttcctct tttttccttt ggcacattaa aatacgcatc
      601 aataaccttg tttccgagta caacaatgcc ttcaatatcc tcgaaagttt cagtaggcag
      661 tcctatcata aaataaagct ttacccctgt ccatcctcca ttgaaagcta ttcctgcgga
      721 attcaacaga tcttcctcag taaccccttt attaattaca tctcttagtc tttgtgtacc
      781 tgcttcaggt gcaaaagtaa gcccgctttt tcttactttc tgtgcctttt ccattagttc
      841 aagtgaaaaa gaatctatac gcagggaagg aagggataaa cttactccct tttcgcacat
      901 attttccagt aacccttcag tcaaatcttt aagtccggta taatcacttg tactaagtga
      961 agcaagagat atttcctcat agcctgtagc agcctccaat ttttcagcaa tttcaatcaa
     1021 cttctcaggt ttcctctccc ttacgggtct atatacaaac ccggcctgac aaaatctgca
     1081 gcctcttgta cagcccctga aaagctcaag cattattcta tcatgtacta tatttacata
     1141 tggcacaata attttttcag ggaaatatac attctcataa tcttttatta ttcttttttt
     1201 tattttagct gggtactcgc tgtttttagg ctgtatttta ttaactgtac catcataatt
     1261 atattttacc tcataaaaag atggtacata tactcctttt attttagctg ctatatctaa
     1321 aaaggattcc cttgtgtatt cagtttcaat tctagaacta atcatgttct ggctggaact
     1381 gattcttttt tgcttccatt ccttatatgc atcaaggatt tcattaataa cctcttcacc
     1441 ttcaccaagc acaaaaaaat caatcacctc tgctaaaggt tcaggattat atgcacatgg
     1501 acctcctgca ataataaatg gatgtgcgtc cgttctatct tttgccctta taggaattcc
     1561 tgcaagatca agcatgttta taacattggt atagctcatt tcgtattgca aagtgaatcc
     1621 aacaaaatca aagtttttta caggttcttt agtttccagt gaaaataaag gtatgctttt
     1681 ttcccgcatt ttactttcca tatctgtcca gggtgcaaat accctttcac aatatgtatc
     1741 atctctttca ttcaaaagat ggtacaatat cttcataccc aggtgggaca ttcctacttc
     1801 atataaatca ggaaagcaaa aggcaaatct aatatccata tttccaggat tttttttaac
     1861 catattccat tcattaccgg tatatcttgc aggtttctca acctgtatta aaatattatc
     1921 atcaatagaa attgacatag caatattatt cctccatatc tccaatgcgc caaatgaatt
     1981 gtcccattga attgttatgt aaaacttcat taattacatg attttacaat taaattgctt
     2041 ttcttgagta aatttttcac aattaagctt ttcacactat ttataatacc caataagata
     2101 aattatagca atatatacct aaaaatatag ttccttaatt tgccttaatc tatccctgtt
     2161 taatctatcc ctgatagata tgataatcgc ttgctttttc tattatataa tcacggaatt
     2221 gctgaactgc tttagtcata taccgcctgt catcccaaat caaatatgtt gtgcgtgtga
     2281 atttttcacc ctgtattggc aggaatatga cattttcgtt ccataatccc tgccatgaaa
     2341 atgatggcac aaaagaaact cccataccaa gagatacata tttccgtata taatacggat
     2401 catcgcaaat aattgaaatt ttaggttcaa agccatgtgc acggcagtat tccttcacag
     2461 tttgagataa actgtactgc gacggcaggg ttataaatcg ctcatccttc aaactgttaa
     2521 gagtgacaga cttatttttc gccagagcat ggtgataagg aactgccagc ataatttcct
     2581 cacaaattaa cggaattccc ttacgtaaat gcggttgcat ccttcgtgac gaaacgcaca
     2641 ggtcaaaatt aaaactatga tctcctatgt tattgtggca gattgaaaac gatacctccg
     2701 gatttaacct ggcaaattcc acaatccaat ctataatcat attacggttt tccaacacca
     2761 ggaggcttat ttcacccttt atgtccccat ttttttcctt aagagccatt ataccgtcac
     2821 ttaaattatc caatactgct tcaacatgct taagaaacag ctttccattt tcatttaagg
     2881 ttaaccggtt actttttctg tcaaaaagct gtgttcctag ttccttttca agcctagata
     2941 tggtttgtga catagctggt tgaggaatac catattgttg tgcagccctt gtaatacttt
     3001 catatcgtgc tgcagaataa aaatatttaa gctgcaaaag ctccatgtac ttcacccctt
     3061 acgaatatat gataacatgg tatagcatac agttcaatgt atgctggcat atataatcac
     3121 atattttaca aattcatatt taatggtaat atttatatag ttatataatg aataagcaat
     3181 gactaggaaa gtagcttaaa gtgaggtcga aaaaaatcat gtatgaaatc aaacgaatat
     3241 tagacattac atatagtaaa gtacaccctg aggattgtaa attagactta tatttgccag
     3301 aaacaaaatt accctgtcct gtacttattt atatacatgg aggaggactt gaagcaggaa
     3361 cccgcaggaa taccagcgga tggattgaag ctttagtgtc tgaatgggga attgctgtag
     3421 tctctctgga ttaccgcatg tatccgaacg cacacttccc cgattttatt gaggattgtg
     3481 ccgaaggtac tgcatgggta accagttatg gacgcaccca cagttgcttt aataagtatt
     3541 atattggcgg ttcttcagct ggagcatatc tcacaatgat gttgtatttc gctgagaatt
     3601 ttcttttgaa gcatggctta tcacccgatg aatttgatgg ttacatattt gatgccggtc
     3661 aacctacaac ccatttcaat atactcagag aacgtagaat tgataccaga gcaatccgtg
     3721 ttgatgatgc tgctcctgtt ttctatgtag acaaacaaat tgaagagcct gagaaaaagc
     3781 ctcctgtact ggttctggtt gctgaaaacg atatacccaa tcggcttgaa caaaacgaaa
     3841 tgtttattgg tacaatgatt aatttcggtt ttatcccgtc ccgtattact aaaaagtata
     3901 tggcaggctt cagtcattgt ggctatgtca atgcaaggga tagtgaaggt aaatccatat
     3961 ttgctaatat tttagctaat tttatagata gaggtaacta agatgaaagg ggtagccaaa
     4021 atgaaaaaag gattaatata tattatttta actgcaattt tgtttacaac attagagcct
     4081 gtaagtaagc tgatttccac agaaataagt cccgtacgca taacatttat aaggtttttc
     4141 ataggcagtc ttatgctttt gcctttttcc atagctcaaa tcaaaaagga aaacattaaa
     4201 ataaatacaa aagacattta tacaatgtca ttcttaggaa tattatgtat ctgcgtaagt
     4261 atgattttgc tgcaatacgc agtactaaaa gcagagtccc ctgcacttat tgcaataata
     4321 ttctcatcaa attcggtgtt tacaataatt tttgcagcat ttattttgaa agatgaaatt
     4381 acttgggcaa aactttgtgc tattgcacta tgtcttgcag ggctgctggt ttccactgat
     4441 tttaaattat cggaaaatat gctttcagtt gtacttgcaa tactagctgc actggcattc
     4501 agtttataca ccgtactcag taaaaagttt atgacaaaag ttagcggaat aattcaaaca
     4561 ggattttcct tttttattgg aagccttgta cttttaatag cccttttgat aggtggcaca
     4621 gatatctttg gtggtattac ttcatcaaat atttgggaac ttctttatct cggtgtagct
     4681 gtaacaggca taggatattg gtcatatttc cgtgcaatgg acaaagcttc tgctatgtcg
     4741 gcatcctttg ttttttttat taaacctgct ttaacacctc ttgcaacatt tgttattaac
     4801 ggtatagtac cggacataaa agtgtttata tcccttgttc ttgtgattac agggtcttat
     4861 atggcaacag ttaaaacaca accaaaaaac aaccttacta aagcatgatt tgcacctatt
     4921 agtaactaat actgtcaata ttatttcttt tttactcttg cacttttgtg ggtttgtgtg
     4981 ttagaatata taagaatttg tggtacagaa aatattgggg tgtagctcag atggtagtcc
     5041 atttgaatct atttcacaac ctgtattatc tacactattt tccttaaata cattaattgt
     5101 taccttatat tcaatctcca cgctatctct ataaataacc actttttcaa ttatttcatt
     5161 tagcatacct tttttagtta aaaaatccgc tttgttatat ctatcaacaa atgatacaat
     5221 attttcatct aatttactat actcatctag agcaattttg gattgcttta aattttcatc
     5281 aaccgtattg atttgagtaa gtaattcatt tacctcagct ttaataatat cttttgattc
     5341 ttttagttct tctagtgtta aaatgtattc atttctaatt gcttttggga tattcttttt
     5401 taatattaat aaatcctttt gcttgtcttc taacattata ttgcgccctt gatttggaag
     5461 atttaagaaa catacaggat aattacttta atgatgacaa gactctgtta tccttccctg
     5521 atggaagaaa aattaggctt actggtaagc ttagtgaatt aataaatgat ctaatcaatt
     5581 ataaaaagat aaataaaatt cgttgtatat atttattttg cagaaaacat gaaaaagact
     5641 atgataaaat atctaagtcc aattataata caataataag taaagccttt aataagctag
     5701 atttacctga agatagaaag aaagtattaa ttccttcatt cataagatct tcagttattc
     5761 gcaaaatgtt tgattcagga ttcagtattt cagtattgaa gatatagtct gccttactgg
     5821 cttaagtttg acaactatta atcagtattt atcatatgag gacatattaa aacgccataa
     5881 attaaaaaag atagtgaaag atcatccata taaggagttt tttgtataag cagctgctga
     5941 cataaatagg taggtacttc ttatgctact tattttttta caaggctata ttttcctcgg
     6001 cttatttaaa ttatttatct ctattcctta ttgcctcact ttgatctgac tttaattgtg
     6061 ctaattcatc aaacaaatct gcaatataat atttatagat actatattta tcttcaatta
     6121 ttgcttcttt aaactgatct aataatttaa taatcttttc ttttttggta tccatatcct
     6181 tccccttgca ttaagtattc atctcacact tatggtaaat atataaaatg tttctttggt
     6241 ttattacata gattgccata ttatgctagt acttgctact aatcaatttc aactcaagga
     6301 taggcacttt tatgggagac tttgttttag acatcaaatt cataaattcg cattctactg
     6361 catatttatc ctctaagtaa ttaactattt tatattcata tttatcatat atttcatcaa
     6421 tcaaaaaagt aacatcagag attactaatg tgtttttttt cttagataaa aattttttta
     6481 tttcttttag agtataaatt tttcctatct ccattttctt acccccaatt taaataaaac
     6541 aacatcaatt aataataacg tcatttaata ataaaaagtt tataataaga tattacttat
     6601 ttagtctatt actaatttcc ttaattttgt tattaacttt ctctaatgca aaaatatacg
     6661 cattgatttt ttacatgctt ctaaagattt ttctaatagc ttttcactaa gctctttttt
     6721 ctggaatcct acatccttaa tataaatcat tttttttgta atttttataa ccttgtataa
     6781 taggttttac tatattttct agcgcttcta ttatttcctc tttaatttca ttataaattt
     6841 ccaaatcaat ttcatcatca taaccaattc cgctattgct aattaagata ttatttaaaa
     6901 ccaaattaca ttttt
//
LOCUS       DAOSDD010000229         6839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4048_length_6839_cov_4.657577, whole genome shotgun sequence.
ACCESSION   DAOSDD010000229 DAOSDD010000000
VERSION     DAOSDD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4048_length_6839_cov_4.657577"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..979
                     /locus_tag="PK733_15915"
     CDS             131..979
                     /locus_tag="PK733_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ12058.1"
                     /translation="MIEKDKLKQLEKKSTLIRKHIIDEVYNAASGHPGGSLSCTDIFT
                     VLYFHEMRVDVNNPRWPDRDRFVLSKGHCAPALYAALAEKGFFPVEELNKFRSIESYL
                     QGHPSMNDVPGVDMSTGSLGQGISASVGMALAGKLDNKDYRVYSILGDGELQEGQVWE
                     ALMSAAHYKLDNLTAFLDHNGLQIDGKITEVMSPEPVVDKFKAFGWNVIVIDGHNYEQ
                     IINAIETAKETKGKPTMVVAETIKGKGVSFMENAAGWHGSAPNKEQRDQAIAELDAFL
                     AELEVE"
     gene            981..1940
                     /locus_tag="PK733_15920"
     CDS             981..1940
                     /locus_tag="PK733_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOJ12059.1"
                     /translation="MADNNTAVKKIATREAYGNALAEFGADSRIVVLDADLSKSTKTD
                     SFKKKYPERFINMGIAEANMMSVAAGLATCGKTVFASSFAIFAGERACEQVRNSICYP
                     RLNVKIGATHAGISVGEDGASHQAVEDMGIMRSIPNMTIISPADAVETRHAVKAAIEY
                     DGPVYLRLGRLALPVIYDESTYKFEWGKGVTISDGSDVTIIATGLMVHEALTAKDMLA
                     SEGISARLIDIHTVKPIDKDIIIKAAKETGAIVTAEEHNMYGGLASAVCEVVAQNYPV
                     PVKVVAIQDKFGKSGKPAPLMKVYGLTAEDIVNKAKEAIKIKK"
     gene            2239..3282
                     /locus_tag="PK733_15925"
     CDS             2239..3282
                     /locus_tag="PK733_15925"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ12060.1"
                     /translation="MFLKLIDKQAKKYFGNAYDIKICDDGSLWPVRFTDKLFSVYGID
                     EGKRIRSFCPSGVCIDLITDSAFLNLHISTLSFARDFAYFDLYIDDVFIGTYGAEPVK
                     QLPNIMSFELNHKHINGKCKGDGKKQRITIYLPQLVDIHIKGIEIEDGSIVEDCSIME
                     NCSNISGHKKTLLCLGDSITQGMTAKSPSSTYPVQLARKLGMNMINHGIGGYKFDPKV
                     LDEDMAVKPDIITVAYGINDWYQYSSIEYFREMCRGFFEKLVKTYPDAKIFTVTPLWC
                     SSEKETKAMGYFCDIRREIEKIAGNFEGIHIVDGLKVVPNAPEYFVDGVHPNDEGFMH
                     YSINLLNEIERYC"
     gene            3313..3582
                     /locus_tag="PK733_15930"
     CDS             3313..3582
                     /locus_tag="PK733_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12061.1"
                     /translation="MNENLKRLIVPANFDPFTNQEIFDIVNKIYDLNTPDNLIKYFDT
                     PVKCLLRIERSIELWTKELGAKGYFEFIEEQLGHIVEKDEKWILL"
     gene            4066..4974
                     /locus_tag="PK733_15935"
     CDS             4066..4974
                     /locus_tag="PK733_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12062.1"
                     /translation="MDKKIPVASIVKYRYLYLLLLPGVIWLIIFRYIPMYGIVIAFKD
                     YNIFKGIGQSPWVGWVNFTEFFRYDRFWVLIRNTVLISVYKIIFGFPFPIIVALMLNE
                     VRGNVFKRSIQTVIYFPHFLSWVIVGGLVITLLSPDGFVNSIVKALGGQSQMWIAKSE
                     YFRGILVLSDIWKEAGWGTVVYMAALSNINVEMYEAAIIDGAARLQRIWYITIPCLMP
                     TIVILLILRMGNVMEAGFEQVLILQNPNVLSIGDIIDTYVYRVGLEQRKYSYSAAVGL
                     FKSIVNMILILITNYSAKKMGQESLF"
     gene            5144..6034
                     /locus_tag="PK733_15940"
     CDS             5144..6034
                     /locus_tag="PK733_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12063.1"
                     /translation="MSKNSIFVSNLKDKIFYTIIYTLLIIIGLLVIVPMLYCISVSLT
                     PYEEALRRGGIILWPSEATLQYYRYVLNRNSPVLRAYGITVLVTIIGTFSSLMITVFT
                     AYPLSKKYLPGRTVIMYMFFITMLFNGGLIPTYLTVRSLGMVDKLSALIIPSLISVFN
                     MIVMRSFFISIPDSLEESARLDGCNDIGVLFKIVIPTSIPAMATIGLFYAVSKWNMFF
                     DAMIYINNRKKFTLQVILREILVMSQVDELNPEMKSSLPPLLPMQMTCTMIAVLPMMI
                     LYPFIQRYFVKGMMIGAIKA"
     gene            6097..>6839
                     /locus_tag="PK733_15945"
     CDS             6097..>6839
                     /locus_tag="PK733_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12064.1"
                     /translation="MVKARKLMVVCMAVLLVLSLSLTACVTQKVSNDGEKNNASGIKE
                     QNDTSKTDQPQKLINLSFFSDDSPNELKLKEVEKLFNIKLNLISTPRQEYNQKLNLAM
                     ASGDVPDIVLFRDIVEMFSYAKQGALLQLDGLLEKNAPNTMAYVAEKLWPYIKVDGKI
                     YTIPTDRIPQKNNVYVRKDWLDRLGLQIPKTLDEYYNIMKAFVNQDPDGNQKNDTYGY
                     GSSAAGNLAVIGTFTPIYGAYGVMDGAWY"
BASE COUNT         2331 a          964 c         1423 g         2121 t
ORIGIN      
        1 agcactgtaa cgacagcccc tggtaaatat ttcttcaaat tcagtaaatg cacccgatta
       61 gcattctccc cttgtcacat aattaatggt atagtatact aaggtaagaa atttattgcg
      121 gaggggttgt atgatagaaa aagataagct aaaacagctt gagaaaaaat ccacattgat
      181 aagaaaacac ataattgatg aagtctataa cgcagcttca ggacatcctg gaggctcact
      241 ttcatgtact gatatcttta cagtgctcta ttttcacgaa atgcgggtag atgttaataa
      301 tccccgttgg cctgacagag accgttttgt tttgtcaaaa ggtcattgtg cacctgcact
      361 atacgctgcc ttggcggaga aaggcttttt ccctgttgag gaactgaata aattcagaag
      421 cattgaaagc tatttgcagg gacaccccag catgaatgat gttcctggtg tagatatgtc
      481 tacaggttct cttggacagg gcatttctgc ttcagtgggc atggctcttg ccggaaaact
      541 tgataacaaa gattatagag tttattcaat acttggtgat ggtgaacttc aggaagggca
      601 agtatgggag gcattaatga gtgcagccca ttataaactt gataacctta cagcttttct
      661 tgaccataat ggattacaga ttgacggcaa aataaccgaa gtaatgtctc cagagccggt
      721 tgtagataag tttaaggctt ttggatggaa tgttattgtt atagacggcc ataattatga
      781 acagataatt aatgctatag aaacagcaaa agaaactaag ggtaagccta ccatggttgt
      841 tgcagaaaca ataaagggca agggagtgtc atttatggag aatgcagcag gatggcatgg
      901 ttcagccccc aacaaggaac aaagagatca ggctatagca gaattggatg ctttcctggc
      961 tgaattggag gtggaataac atggcagaca ataatactgc agtaaagaag attgcaacaa
     1021 gagaagctta tggaaatgcg ttagctgagt ttggcgcgga cagtagaatt gttgtacttg
     1081 atgcagatct ttcaaaatct acaaaaacag attcctttaa aaagaaatat cctgaaagat
     1141 ttataaatat gggaatagct gaagcgaata tgatgtcggt tgcagcaggt ctggcaactt
     1201 gcggtaaaac agtttttgcg agcagcttcg caatatttgc aggagaaaga gcctgtgagc
     1261 aagttagaaa ttccatttgt tatccaagat taaatgtaaa aattggtgca actcacgcag
     1321 gtatatctgt aggtgaggat ggagcttcac atcaggctgt agaagatatg ggaataatgc
     1381 gttccatacc aaacatgaca ataatcagtc ctgctgatgc tgttgaaaca aggcatgcgg
     1441 taaaggctgc tatagaatat gatggacctg tatatttaag acttgggcgg ttggcattac
     1501 ctgtaatata tgacgaaagt acttataaat ttgaatgggg taagggggta accatttcag
     1561 atggctctga tgttacaata attgcaacgg gtttaatggt gcacgaagcc ctgacggcaa
     1621 aggatatgct ggcaagcgaa ggtattagtg caaggctgat tgatatccat acagtaaagc
     1681 caattgataa agacattatt ataaaagctg ctaaagaaac aggtgccata gttactgctg
     1741 aagaacataa tatgtatggc ggcctggcaa gtgctgtttg tgaagtagtt gcacaaaatt
     1801 atcctgtacc cgtaaaggtt gttgcaatac aggataagtt cggcaaatcc ggcaagcctg
     1861 ctccactgat gaaagtttat ggccttactg ctgaggatat tgttaataag gctaaagaag
     1921 caattaagat taagaaatag tagatagcag atagcggata gtgagcagtg aatagtgagt
     1981 agtgaggcat tagagtaaag gatataagag aaggggacat tgagtacact ccgaacggta
     2041 atgtcccctt tttattttga taattttaaa tgcacccata atttcacatt aatattgata
     2101 tcaaaattat tatcatttaa aataaaagta ttaagcaact gcgctttttt atacattatt
     2161 tgtttaatgt aatgtagtgt aataatttat aataacttta tggcaatatt ttttaatatg
     2221 tttaggggtg aatgaaaaat gtttttaaaa cttatagata aacaagcaaa aaaatatttt
     2281 ggaaatgcat atgatataaa aatatgtgat gatggaagct tatggcctgt aagatttaca
     2341 gacaaactat tttctgtata tggcatagac gaaggcaaaa ggattcgaag cttttgtccg
     2401 tcgggagtat gtattgattt aattaccgac tctgcatttt taaatttaca tatcagcacc
     2461 ttaagttttg cccgtgattt tgcatatttt gatctttaca ttgatgatgt ttttattgga
     2521 acatatggtg cagaaccggt aaaacagcta cctaatataa tgtcctttga attaaatcac
     2581 aaacatatta atggaaaatg taaaggtgat ggaaaaaaac agagaatcac aatatactta
     2641 cctcaactgg ttgatataca tataaagggt attgaaatag aggatggcag tattgtagaa
     2701 gactgcagta ttatggagaa ttgcagcaat atatccgggc ataagaaaac attgctatgt
     2761 cttggagact ccataactca agggatgact gctaaaagtc cttcttcaac atacccggtt
     2821 caattggcaa gaaaacttgg aatgaatatg attaaccatg gaattggtgg ttataagttt
     2881 gacccaaaag tactggatga agatatggct gtaaaaccgg atataataac agttgcatat
     2941 ggtataaatg attggtatca atatagctcg attgaatatt tcagagagat gtgccggggt
     3001 ttctttgaaa aattagtaaa aacatatcct gatgcaaaga tatttacagt aactccatta
     3061 tggtgcagca gtgaaaaaga aactaaagct atgggctatt tttgtgatat aagaagagaa
     3121 atagaaaaaa ttgccggtaa ttttgaaggg attcatattg ttgacggtct gaaagttgta
     3181 cctaatgcac ctgagtactt tgttgatgga gttcatccaa atgatgaagg gtttatgcat
     3241 tatagtatta acttgcttaa tgagatagag cgttactgtt aatactgcaa aaacaaagcc
     3301 ctacaatgta ggttgaatga aaacttaaaa agactgattg taccggcaaa ctttgaccct
     3361 tttacgaatc aggagatatt tgatatagta aataaaatat atgacctgaa cacaccagat
     3421 aacttgataa aatactttga tactcctgtg aaatgtcttc taaggataga aaggtcaatt
     3481 gaactatgga caaaggaact tggagctaaa gggtattttg aatttattga agaacaattg
     3541 ggtcatattg tagaaaagga tgaaaaatgg attttgctat gatgattgaa gaccgggata
     3601 attttgagaa gcttggtatt gatgcttctt cccttacgcg ggaatattct tccaatgatt
     3661 agtaaatctt gtattgacat tatgcgtcta ctaaagctga tagaaaacaa atacctggga
     3721 gggtaaatat atataatgca atgcttatta tatctgaaga catggggtat tcttggtcgt
     3781 agctaaacga attaatgcaa gtgattatag agaactatat tctaataaat tacacaatta
     3841 agaaatctta caactatact tcctatgatt tattagtatg gttatggtat tatttggttg
     3901 actcatattc taataacata actaaataag tctattaaaa tcatatctgt ctattaataa
     3961 aagcttaata aactgtatgg agaatttaca aataggcaat ttggctatag tataagtatt
     4021 aagcaattat atgcgaaaca ctagaaaaga ggcaggtttg ctataatgga taaaaaaata
     4081 ccagttgcaa gcatagttaa atatagatat ctgtacttac tactattacc tggggttata
     4141 tggcttataa tatttcgtta tattcctatg tatggaattg tcattgcttt taaagattat
     4201 aatatattta agggtatcgg gcaaagcccc tgggtgggtt gggttaattt cacggaattt
     4261 ttccgttatg ataggttttg ggtgcttata agaaatactg ttttaataag tgtttataaa
     4321 attattttcg gatttccatt tcctataata gtagctttaa tgcttaatga agttcgaggt
     4381 aatgtattca aacgttcaat acagactgtc atatattttc cccattttct ttcctgggta
     4441 attgttgggg ggcttgttat taccttattg tcaccagatg gttttgtaaa tagcatagtg
     4501 aaagccctgg gcggtcaatc acaaatgtgg atagcaaaat cagagtattt tagaggaatt
     4561 ttagtactat cagatatctg gaaggaagct ggttggggta cagtagtata tatggcagca
     4621 ttgtcaaaca ttaacgttga aatgtatgaa gctgcaataa ttgacggggc agcccgctta
     4681 caaagaatat ggtacattac aataccatgc ttaatgccta caattgttat attacttatt
     4741 cttagaatgg gaaatgtaat ggaagcaggt ttcgaacaag ttttaatatt gcagaatcca
     4801 aatgttttaa gtattggaga tattattgat acttatgtat acagggtagg gctggaacaa
     4861 cggaaatata gttattcagc tgcagttggc ttatttaagt ccattgttaa catgatatta
     4921 attcttatta caaattattc tgcaaaaaaa atggggcagg agtccttatt ctagataaaa
     4981 tgtaaaaatg atttagcaag gaatgtattg gattttcgtg tattaattaa ggtaatataa
     5041 ttgtacaaaa atttttattg ttaaacataa gagccaaatg taggaataaa agtaaaagaa
     5101 atattgaaat gagccaattt attttggcag aatggcggtc aatgtgagta aaaattcaat
     5161 atttgttagc aatttaaaag acaagatatt ttatactata atatatactt tgctcataat
     5221 aattgggttg ttggtaattg tacctatgct atattgcata tctgtttctc ttactccata
     5281 tgaagaagca ttaaggcggg ggggaataat tctatggcca agcgaagcaa ctttacagta
     5341 ttatagatat gtattaaata ggaattcgcc ggtacttcgt gcatatggga taactgtcct
     5401 ggtcacaata ataggaacct tcagcagtct gatgataaca gtattcactg catatccttt
     5461 atccaaaaaa tacttgcctg gaaggacagt gataatgtac atgtttttta taacaatgct
     5521 tttcaatggg ggactgatac ctacatatct tacagtacgt tcattaggta tggtagacaa
     5581 attatctgct ctgattatac cttcgttaat tagtgttttt aacatgattg ttatgagaag
     5641 cttttttata tctattcctg atagtcttga ggaatctgcc cgattggacg gttgtaatga
     5701 tataggtgtt ttatttaaaa tagtaatacc cacatcaatt cctgccatgg caactattgg
     5761 cttgttttat gcggtaagta aatggaacat gttttttgat gctatgatat atataaataa
     5821 tagaaaaaag ttcacactac aagttatact gagggaaatt cttgttatga gccaggtaga
     5881 tgagctaaat ccggaaatga aatcgagctt gccaccattg ctacctatgc aaatgacttg
     5941 cacgatgatt gccgtcttac ctatgatgat attatatcca tttattcaga ggtattttgt
     6001 taaaggtatg atgattggag cgatcaaagc ataattacag tggtaataaa accactgata
     6061 aattataata aatttatatt acaggagggt tgtattatgg taaaggctag gaaattaatg
     6121 gtagtttgta tggcggtttt attagtatta agtttaagtc ttacagcttg cgtaactcaa
     6181 aaggtaagca atgatggtga gaaaaataat gcaagcggaa ttaaggagca aaatgatact
     6241 agtaaaacgg atcaaccaca aaaattaatc aatctttctt tttttagtga tgattcgcca
     6301 aatgaactaa aattgaagga ggtggagaaa ctatttaata ttaagctaaa tcttatttct
     6361 acacccaggc aggaatataa tcaaaaacta aaccttgcca tggcttctgg tgatgtacct
     6421 gatattgttc tttttagaga tattgtcgaa atgttttcat atgcaaaaca aggcgcactc
     6481 ctgcaacttg atggacttct tgaaaaaaat gctcccaata caatggctta tgtggcagaa
     6541 aaactgtggc cttatataaa agttgacggt aaaatctata ctataccgac agacaggata
     6601 ccacaaaaaa ataatgttta tgtcagaaaa gattggttag atagattagg tttgcaaatt
     6661 ccaaagactc tggatgaata ctataatatt atgaaagcat ttgttaacca ggatccggat
     6721 ggcaaccaga agaatgatac ttatggttat ggaagctccg cagcgggaaa tttggcagta
     6781 ataggaacct tcacgccaat atacggagca tatggagtga tggatggagc atggtactg
//
LOCUS       DAOSDD010000230         6815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4069_length_6815_cov_4.919822, whole genome shotgun sequence.
ACCESSION   DAOSDD010000230 DAOSDD010000000
VERSION     DAOSDD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_6815_cov_4.919822"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1619
                     /locus_tag="PK733_15950"
     CDS             225..1619
                     /locus_tag="PK733_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ12065.1"
                     /translation="MAKKPNLLFFGIDSLRSDHMSLYGYKHLTTPHIDKYAKGGIVFE
                     NCFSPSIPTTSGYSCMFTGKDCFGTNVVALRHKGGIADGVKTLAEVLNENGYNSTCIG
                     FGGNPASRGFGKYIEYEGWGSWEKGRSPKAENLNKVAIPELERLAKEDNPFFLFMRHM
                     DPHSPYLPPEPFHKIFYGADEFDPNNKSLGPVYEFKPFRDYFYTWFAPGCTDKDYIIA
                     QYDGAVAYMDACIQNILAKLEALGLEEETLVVFTSDHGETLYDHDCYFDHHGLYDCTL
                     TVPLVLRFPGKLPKGKRYPDYCQLKDIMPTIMEILGVDTGIDFDGRSLMPLTRGEYRE
                     AEPEFYITECTWMRKHGWRTPEWKLIHALEPDFHFKPEVELYNLIKDPEENNNVADKE
                     LGVVKMLKERMQQYIAKREKETGRENPMYNNLDWHGKGCGPFKTSQQAYDSLHIGDPE
                     AAKKLQAALAQKKT"
     gene            1811..2818
                     /locus_tag="PK733_15955"
     CDS             1811..2818
                     /locus_tag="PK733_15955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12066.1"
                     /translation="MLKACIIGMGNIGNTHARVYTGCDKAELAGVCDIIPERAEKAGK
                     KYGVPFYLNAEDMLKNVKPDICSITTGGFEYSSDHYIPTIQALKAGCNVLCEKPICND
                     LEKAQIMVNTAKKLDRCFAINLNHRFTPAARIVKQWQNEDRLGHLLFINMALWIGRFE
                     PLESEYYHLKALNPHSVDIMRYFCGDVDTVHCFAIKAPGRNIWSTASINMKFKNGAVG
                     HLTSSYDIKRGHPMERCEIAGTKGRAIFEDMWREAVLYPADSLVKQVYTNPVFGSFSD
                     FYDTFRDRIICFVDQVARGDKPEDIDGSGEQGLEASRVIHAAIESIKTGFPVKVESIR
                     K"
     gene            3014..3874
                     /locus_tag="PK733_15960"
     CDS             3014..3874
                     /locus_tag="PK733_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12067.1"
                     /translation="MAEVRFLEKSKFFEDKNIPILVMDTEHHGRVGLHSHDFIEIVYI
                     DKGFSMHFCEGETTILTSGDLFAIRPGEVHSYNSAHHTFLYNCLFCMEALEGISSEIA
                     KLPGINQIFQQKQGKWEKLHLDFSERREVLLYLEKMKWEQVNRSIGWELNMKSLLTSF
                     LVLYSRLYSNHHESLDKNNKAQILHVHKALKYIEENYRLDFSLKEMGASINLTPDYIS
                     RQLKSAVGLSTIEYLKNFRIAKAIEQLKTTELPISEIAQNTGFGDLSNFSRQFKQVVG
                     VSPSAFRKDE"
     gene            4068..5165
                     /locus_tag="PK733_15965"
     CDS             4068..5165
                     /locus_tag="PK733_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12068.1"
                     /translation="MALKIGIVGMGGIGNLHAECHKKDPLAELVAVCDIIREKADAAA
                     ERHGVKAYYSLKEMLKAHPELDVIDVTTSGYENGSWHYVPVMEAIDAGKNVLTEKPIS
                     NDVAEAREMVRFAAERDVYLGCNLNHFFTEPADRAMKYIKDGRVGEPVYCLHKVGFNG
                     GEANYGGPGSPRWQRPYSHVKAFLAHPFSVMRYFCGDITHVQAFLNKPGVRKSADDLM
                     LSINSVHVRFENGSVGYLLSQRGDAMFGLGGWWSFEMAGTKGTFCIENCVEQLTFWDS
                     PKKGEKTPAAPEILNTGITDFNSTFPTRLHAFLEDITNGVPKEFLRASGRDALATLEY
                     TFAAIESYEEGGALVRPNRLPNVHGHPSVVW"
     gene            5397..6749
                     /locus_tag="PK733_15970"
     CDS             5397..6749
                     /locus_tag="PK733_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ12069.1"
                     /translation="MKKIISILLIIVMMFSIAIFSSSCGNRGKDDGSTKSGSLSDKSP
                     SSSTVSSSADSTSSTNSAVSKEPVSLTFLRIGNDAPEAEYWKSLIKDFEEKNTNIKIQ
                     YDDAAIGEPMETKLNTLFAGGAGPDIIGHGILSVANRVEAGHYTPISEYFSKWDGKED
                     IMESVLANGTYKGEVYGLAYSTTPFVFAYRKDMFKDAGLDPEKPPQTWDELKDYAKRL
                     TVKTGDRITRAGFAFPMAAGNFVEFDVFVFGNGGRFYDENNMPTINTPEKAEVFEFLK
                     GFIDEVNIPYNSNEVNPFIKGNAAMTLINNVALRPVLADPELKDKIGVAVPPYNKKKA
                     TFSGCNMLFIGGDCKNKDEAWKFIEAALTKEQIIERSKKLNIPVTRKSLIDEFAKLDP
                     YNAVRAECVSAGIGMPRTTWSPRFQAIRNEMVQKVLYSKASIQEALTEAQNKLMEEIK
                     "
BASE COUNT         2261 a         1150 c         1494 g         1910 t
ORIGIN      
        1 aggttattgg ccctgaacag gatatgacat ctgcatcaat tatagcagct gagagctatg
       61 gatacatgaa tgcctgtctt aaaatgctgg gggcaagata gaggaaatat tatttaagaa
      121 aagtcctgca gttgcagctt atttttttac tatacctgaa ttctaaattg actattttat
      181 tacaatttat aaagtgagat tatattttga agggggatat taaaatggcc aaaaaaccca
      241 acctgttatt ctttggaatc gacagcttaa gatcagatca tatgagcctt tacggctata
      301 aacatcttac aacgcctcat attgataagt acgcaaaggg gggcatagta tttgaaaatt
      361 gtttcagccc aagtattcct actacatccg gatattcgtg catgtttaca gggaaagatt
      421 gctttggaac aaatgttgtg gcattaagac acaagggcgg aattgctgat ggtgtgaaaa
      481 ctctggctga agtacttaac gagaatggat ataactcaac atgtatcggc ttcggcggta
      541 atcctgcatc ccgtggattc ggcaaatata ttgagtatga aggctggggt tcctgggaaa
      601 agggacgctc tcccaaagct gaaaatctta ataaagtggc tattcccgaa ctggaacgcc
      661 ttgcaaaaga agataacccc ttcttccttt ttatgcgtca tatggaccct cattctccat
      721 atcttcctcc agaacctttc cataaaatat tctatggagc ggatgagttt gatccaaaca
      781 acaaatctct tgggcctgta tacgagttta agcctttcag agattatttc tatacctggt
      841 ttgcaccggg atgcacggac aaagattata taattgctca atatgacgga gcagtagcct
      901 atatggatgc ctgcatacag aatatattag ccaaactgga ggctttgggg ctggaagaag
      961 aaaccctggt tgtttttaca tccgaccatg gtgaaactct ctatgatcat gattgttact
     1021 ttgatcatca cggactctat gattgtacac ttacagttcc gctggtgctt cgttttcctg
     1081 gaaaactgcc gaaagggaag agatatcctg actattgtca gcttaaagac attatgccca
     1141 ctataatgga gatactgggg gtagacacag gcattgattt tgatggaaga agtctgatgc
     1201 ctttaacaag gggagaatac agagaagcag aacctgagtt ttatataacc gaatgtactt
     1261 ggatgagaaa gcatggctgg agaacacccg aatggaagct tatccatgca ttggaaccgg
     1321 atttccactt taaacctgaa gttgaacttt acaacttaat taaagaccct gaagaaaata
     1381 acaatgtagc ggataaggag ctgggggttg taaagatgct taaagaacgt atgcagcagt
     1441 atatagcaaa gcgtgaaaag gaaaccggaa gagaaaatcc catgtacaat aatcttgatt
     1501 ggcatggaaa gggctgtggt cctttcaaaa catcacagca ggcgtatgac tctttgcata
     1561 ttggtgaccc tgaagctgct aaaaagctgc aggctgctct tgcacagaag aaaacttaat
     1621 tgatagaaat tacctgacat ttaacttaat tattgctcaa tgaaatagaa atgaggagtg
     1681 catttatcaa atatataaaa atgtatacca ggggctgtcg ctacggtact tagagcacct
     1741 ccacttatgc cccttatcaa tattttcttt atttaaattt aatgcaccca gaatgggaga
     1801 atgattaaaa atgcttaaag catgtattat tggaatgggg aatataggaa acactcatgc
     1861 gagggtatac actgggtgtg ataaggctga actggcagga gtctgtgata taatccctga
     1921 gagggctgaa aaggcaggta agaagtatgg tgtgcctttt tacctaaatg cagaagatat
     1981 gcttaaaaat gtaaagccgg atatctgcag cattaccact ggtggttttg aatattcaag
     2041 tgatcactat atccctacta tacaagcgtt gaaagcgggg tgtaatgtgc tgtgcgaaaa
     2101 accaatttgc aatgaccttg aaaaagctca aattatggtg aatacagcta aaaaactaga
     2161 caggtgtttt gccataaacc tgaatcatcg ttttactcct gctgcacgca tagtaaaaca
     2221 gtggcagaat gaagaccgcc tgggacatct tttgttcata aatatggctc tttggatagg
     2281 aaggtttgaa cctcttgaat ccgagtatta ccaccttaag gcacttaacc cgcacagtgt
     2341 tgatataatg cgttatttct gcggtgatgt ggatactgtt cactgttttg caataaaagc
     2401 acccggaagg aatatctggt caacagcttc aataaatatg aaatttaaga atggcgctgt
     2461 aggtcactta acaagttcat atgatataaa acggggacac cctatggagc gctgcgaaat
     2521 tgcgggtaca aaggggcgtg cgatatttga agatatgtgg cgggaagctg ttctatatcc
     2581 tgcagatagt ctggttaagc aggtttacac taatcctgtc tttggaagct tcagcgactt
     2641 ttatgacact ttccgtgaca ggataatctg ttttgtagat caggttgcca gaggtgacaa
     2701 acctgaggac attgatggtt caggtgaaca ggggcttgaa gcaagccgtg taattcatgc
     2761 tgcaattgag tctattaaga cagggtttcc tgttaaggtt gaatcaataa ggaagtaaaa
     2821 gaaagaggaa taaatcataa taaagaagcg ttaaagaaag cagcgataaa ataaagaagt
     2881 gttaaggaaa tattaaagat taaagaaagc agcgataaaa cagaatatag agaaggctta
     2941 aagtagtatt agagcagaac tgtaaagtaa aatgtcacaa aataacttat agagagtggg
     3001 gcttaagggc ttaatggctg aggtaagatt tttagaaaaa agtaaatttt ttgaagataa
     3061 aaacatacca atacttgtta tggatactga gcatcatgga agggtaggtt tgcacagcca
     3121 tgactttatc gaaattgttt acattgataa ggggttttcc atgcatttct gtgaaggaga
     3181 aaccaccatt ctcacatcag gcgatttgtt tgctataaga cctggggaag tacattcgta
     3241 taatagtgca catcacacat ttttatacaa ctgtttattt tgtatggagg ctctggaagg
     3301 catttcatct gaaattgcca aactgccggg tataaaccag atatttcaac aaaaacaagg
     3361 gaaatgggaa aagctgcacc ttgatttttc tgaaagacgc gaggtcttat tatatcttga
     3421 aaagatgaaa tgggaacagg ttaatcgcag tataggctgg gaactgaata tgaaaagctt
     3481 attaaccagc ttcctggtat tatattccag actatatagc aatcatcatg aaagcttgga
     3541 taaaaacaat aaagctcaga tacttcatgt tcataaagcc ttaaagtata tagaggaaaa
     3601 ctatcgactg gatttttcac taaaggagat gggggcttca ataaacctaa ctcctgacta
     3661 tatatcacgt cagcttaaaa gtgctgtagg tctatcaact attgagtatt tgaaaaattt
     3721 tagaattgca aaggcaattg aacagctaaa gacgacagag ttgcctattt cagaaatcgc
     3781 ccaaaacaca ggatttggag atttaagcaa cttctcacgg caatttaaac aggtagtcgg
     3841 ggtttcgcct tctgctttcc gcaaggacga ataggcaaga atggttatac tttggcttca
     3901 tgaattaggc ttatgaatta gtactacttt gttatgttgc caatgaaaag cgacatcttt
     3961 ccgatgttat aataaattat cccggaagac atcgcttacc aaattggcat attagtaata
     4021 ggcttacata atttattaaa aaaaccatag aaaggaaggt aatatatatg gcactaaaaa
     4081 taggtattgt aggtatgggt ggtattggaa atctccatgc tgaatgtcat aagaaagatc
     4141 ctcttgcaga acttgttgca gtctgcgata ttatcaggga aaaagccgat gctgctgctg
     4201 aaaggcatgg ggttaaagca tattacagct tgaaggaaat gctgaaagct catccggaac
     4261 ttgatgttat tgatgttaca accagtggct atgaaaatgg cagctggcac tatgtacctg
     4321 ttatggaagc tattgatgcg ggtaaaaacg tacttacaga aaaacccata tccaatgatg
     4381 tagctgaagc aagggagatg gtgaggtttg cagcggagag ggatgtttat ttgggatgca
     4441 atctgaacca cttttttact gagccggctg atagagcaat gaagtatatt aaagatggca
     4501 gggtaggtga acctgtatat tgtttgcaca aagttggatt caacggcgga gaagcaaatt
     4561 atggaggacc tggaagtcca agatggcagc gtccatattc ccatgttaaa gcatttctcg
     4621 cacatccctt tagcgttatg cgttatttct gcggcgatat tactcatgtt caggcattcc
     4681 taaacaagcc tggagttaga aaaagtgcag atgatttaat gctctcaatt aacagcgtcc
     4741 atgtacgctt cgaaaatggc agcgttggat atttattatc acagagagga gatgcaatgt
     4801 ttgggctggg tggctggtgg agttttgaaa tggcaggaac aaaaggcact ttctgcatag
     4861 aaaattgtgt agagcaactt actttctggg attcacccaa gaaaggtgaa aaaaccccgg
     4921 ctgctcctga aatactaaat accggaataa ccgactttaa ttcaaccttt cctacaagac
     4981 ttcatgcatt cctggaagat ataacaaatg gagttcctaa agaattcctg agagcgtccg
     5041 gacgggatgc actggcaact cttgaatata cctttgctgc tatagaatca tatgaagaag
     5101 gcggtgcact ggtgcgtcca aacagattgc caaatgtaca cggtcatccg tcagttgtat
     5161 ggtaggtaga aaactcaccg ttgaataagg ttgctatata taacgcacaa aagtaactac
     5221 actataaaag ttttgtgcgc ttatgttacc ttaaataata cacaaaaata caatttgttc
     5281 cttgaaaatc atttttgcgt tatatatata ataaattgcg tcatttcaat tcctaattat
     5341 aaaagaggaa gacttataac ggtttgtaaa aaaagtagtt aatattggag gaaaatatga
     5401 aaaagataat ttctatatta ttaatcattg taatgatgtt tagtattgct attttctcat
     5461 cttcatgtgg aaatagaggt aaagatgatg gttcaacaaa atcaggctca ttatctgaca
     5521 aatcaccatc ctcatctaca gtttcatcat ctgccgattc cacatcatct actaattcag
     5581 cagtatctaa ggagcctgta tcccttactt ttttgcgaat tggcaatgat gctccggaag
     5641 cggaatattg gaagtcctta ataaaggatt ttgaagaaaa gaatacaaat atcaaaattc
     5701 aatacgatga cgctgcaatt ggagagccca tggaaaccaa gcttaataca ctttttgccg
     5761 gaggtgcagg ccctgatata atagggcatg gaattctttc ggtagcgaac agagttgaag
     5821 ccggtcatta tacgcctatt tccgaatact tttctaaatg ggatggtaaa gaagatataa
     5881 tggaaagtgt tcttgcaaat ggtacatata aaggtgaagt gtatggactt gcatattcaa
     5941 ccacaccttt tgtttttgca tatcgtaaag acatgttcaa agatgcaggc ttggaccctg
     6001 aaaaaccacc tcaaacatgg gatgaattga aagactatgc aaaacgacta actgtaaaaa
     6061 ccggcgatag gattactcgt gcaggatttg catttccaat ggctgcagga aattttgttg
     6121 agtttgatgt cttcgttttt gggaatgggg gcagattcta tgacgaaaac aatatgccca
     6181 ctataaacac tcctgaaaag gctgaagtgt ttgaattcct gaaaggattt attgatgaag
     6241 taaatattcc gtacaacagc aatgaagtaa atccatttat aaaaggaaat gctgcaatga
     6301 ctctgataaa taacgttgca ttgcgtcctg tacttgcaga tcctgagttg aaagataaaa
     6361 ttggtgttgc agtacctcca tacaataaga aaaaagcaac attttcggga tgcaacatgc
     6421 tctttatagg tggagactgt aaaaataaag atgaagcgtg gaaatttata gaagcagcac
     6481 taacaaaaga gcagataata gaacgcagta aaaagcttaa tataccagtt actcgaaaat
     6541 cactaataga cgaatttgca aagctggatc cttacaatgc agtaagagct gaatgtgttt
     6601 cggctggtat cggtatgcca agaacaacct ggtctcccag attccaggct ataagaaatg
     6661 aaatggttca gaaagtgctt tattcaaaag catcaataca agaggcttta acagaagctc
     6721 agaacaaatt gatggaagag ataaagtaac aatgggtaaa ggggtgcatt taaaattttt
     6781 gaagaaatat attccagaag ctatcgttac ggtac
//
LOCUS       DAOSDD010000231         6699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4154_length_6699_cov_3.676099, whole genome shotgun sequence.
ACCESSION   DAOSDD010000231 DAOSDD010000000
VERSION     DAOSDD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4154_length_6699_cov_3.676099"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK733_15975"
     CDS             complement(<1..248)
                     /locus_tag="PK733_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ12070.1"
                     /translation="MNSLDLIGMAFKNLWRRKVRTILTVLGVIVGTASIVVMLSLGIG
                     MNESFKNQVSMMGSLNVIRVDTYYYPETIDGEVDYGRPP"
     gene            complement(241..2817)
                     /locus_tag="PK733_15980"
     CDS             complement(241..2817)
                     /locus_tag="PK733_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12071.1"
                     /translation="MKKLSSCICKKLNSTMNKKINGFSLTRVVLACFLIIFQLLAVIV
                     PAGITVKAEDNGVRLVVEEMPNVLPIGEVFAVNVTLHNNTGKAITDIELIIGANEGLM
                     PINATSDRKFLYKNVELPNEQQRSYTIKLVYNGSEDTPIITIGCRCRLEGEEWNQAKN
                     DSDSRIVFFKSDSSGNTPPVTTEKTVPNIKVKSDIKMPAFKAGEDVILSLPVVNDSLY
                     ESRNIIAAIDTSNESILPFTFDQIILDSYIDKILSKKEDKISYNIKIKADAKEGIYPI
                     KLNYQYSNIYNNTFSSGETIYIKVINDKTPPKLIMESIQTYGSDPSPGNIMSLALSLK
                     NQGSLPAKDIKVTLKGLGIDGITTYKSADSKYISQIDGQGKGKAEYFLIISEKFTGNS
                     ASLKADIEYKDGLGNTYSDEFQFFVRINEIKEEEEDLTKPEIALKDLDFPTGKIQAGD
                     DFNLGFILDSVGTDSAQNVKVSLTCDDGILPKSSSVIQIDALEKGQSKELKFNLFASL
                     SAITKNYLIAINVEYENVTEDKENKKDKIEKLTLTRYVGVYVEKKEEEDEDKDKDKEE
                     KTVPKIIINMYSFEPEEVKAGENFKLKLSFLNTSKILPIRNIKITFSAKEGVFIPTSS
                     SNTFFIEYMGTQGIIEREVELLAKSDAPPKSHVLNLIFDYEDEKGTQYNAAEEISIPV
                     KQEQKLVVGQLNLPVEGYEGQPIPIFVDFFNMGKSVLYNLMVTVEGEGFQTENANYFA
                     GNFEPGRSDYFESMITPSSAGEAAGELVFSFEDEAGSKNEVRKEIKLNIMGMERPTMS
                     PEGAMGEEFPGGMPPQDQQKKLLTPLNIGIAGGGLVVIIVVLIIILRKRRIRKAGMTF
                     DE"
     gene            complement(2828..3526)
                     /locus_tag="PK733_15985"
     CDS             complement(2828..3526)
                     /locus_tag="PK733_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12072.1"
                     /translation="MENIIVLQDVRKVYRVGNEKVIALDSISLNISKGECCCFLGTSG
                     SGKSTLLNLMAGLEKPTKGSIIIKNLEIHKLNERKLTKFRQENVGFVFQSYNLMSTLT
                     ALENVSIPLTFKGIPRTIRDKQARKVLKGVGLGTHLKHKPTQMSGGQQQRVGIARAFV
                     SNPEIVFADEPTGNLDSKTTKEVMDLIISMTRKNNQTLIIVTHDIDIAKYADRIFYIK
                     DGRVEKIETINAKN"
     gene            complement(3872..4405)
                     /locus_tag="PK733_15990"
     CDS             complement(3872..4405)
                     /locus_tag="PK733_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOJ12073.1"
                     /translation="MQKLIIIEGDITKIEVDAIVNAANKTLLGGGGIDGAIHRAAGPE
                     LLEECKKLKGCETGEAKITKGYNLPVKYIIHTVGPVWKGGDGREDDILALCYKNSLKL
                     AVDNNIKVIAFPSISTGAYGFPVNRAARIAVNEINNFLEADEDNNISKVFIVCFDSYT
                     YESYINAKKQICNEEFR"
     gene            complement(4523..5281)
                     /locus_tag="PK733_15995"
     CDS             complement(4523..5281)
                     /locus_tag="PK733_15995"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOJ12074.1"
                     /translation="MEDEVNLHVNSSEKNLQNNIKEGIKQKSRIWELDLLRGIALLLM
                     IYFHIIFDMKDIFGYNVYYSKGFNYLTGRAAGILFILISGISCTLSKNNMKRALKILA
                     IAMIITVLTHLYNVDMGIKFGILHFLGLSILTYPLLNNLNEYLLMMSGTVILILGGYV
                     SRLNPGFDYLFIVGITSEKFTSSDYYPLIPWLGIFIYGIILGKVLYKDKRSIFNFQLR
                     NNIISWLGRNTLPVYIIHQPVILAIIGLIKKITV"
     gene            5712..6512
                     /locus_tag="PK733_16000"
     CDS             5712..6512
                     /locus_tag="PK733_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HOJ12075.1"
                     /translation="MIIIGEKINSTLKAVRPAIENYDIGFIQELAKKQFEAGANFIDV
                     NAGMFHDDEPKRLKWLVETVQEVIGTPFAIDSPNPEAIEEALKVNKNGKPIINSITDE
                     KERYEAILPLILEYNTGVIALCMDDSGMPETVDDRLEVAVRIIGKLTDKGVKPQDIYI
                     DPMIRPIGTGSHYGVVALETIRQVKDKYSDVHIACGLSNISFGLPGRKVLNQAFLIAA
                     MTVGMDGAILDPLDKKLMTYLYATQALLGQDEFCMDYLSKYREGLLDL"
     gene            6527..>6699
                     /locus_tag="PK733_16005"
     CDS             6527..>6699
                     /locus_tag="PK733_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ12076.1"
                     /translation="MNVLGVIAEYNPFHNGHLYHLQESIKCTSANFIVCAMSGNFVQR
                     GEPAIINKWARTE"
BASE COUNT         2084 a         1293 c          966 g         2356 t
ORIGIN      
        1 gggggtctac cataatcaac ctctccatct attgtttcag gataataata ggtgtctact
       61 ctaattacat taaggcttcc catcatgctt acctgatttt taaagctctc attcatacct
      121 ataccaagag aaagcatgac aacaattgat gcagtaccca ctataacacc aagcactgtc
      181 aatatggttc tcactttcct tctccataaa ttcttaaagg ccattccaat aaggtcaaga
      241 ctattcatcg aatgtcatac ccgcctttct gattcgcctc ttccggagga taattattaa
      301 tacaactata attaccacca gtcctccacc tgcaattcca atatttaatg gagtcaaaag
      361 ctttttttgc tgatcttggg gaggcattcc tccgggaaat tcttctccca ttgcaccttc
      421 aggagacatt gttggcctct ccatacccat aatattaagt tttatctctt ttctcacttc
      481 atttttactt cctgcttcgt cttcaaagct aaaaacgagc tcgcctgctg cttcacctgc
      541 agagcttgga gtaatcatgc tctcaaaata gtcacttctt ccaggctcaa aatttcccgc
      601 aaaatagttt gcattttcag tttggaatcc ttccccctca actgttacca ttaaattata
      661 gagtacgctc ttacccatat tgaagaaatc aacaaatatc ggtatgggtt gcccttcata
      721 accttctacc ggcaggttta actgaccaac aacaagtttt tgctcctgtt ttacaggtat
      781 gcttatttct tcggcagcgt tatattgcgt acccttctca tcttcataat caaagataag
      841 gttcaataca tgagattttg ggggggcatc agattttgct aataattcta cttctctttc
      901 tattatgcct tgtgttccca tatattcgat aaaaaaggta ttactgctgc tggtaggaat
      961 aaatacccct tcttttgcag aaaatgttat tttaatattc cttataggga gtattttgct
     1021 tgtgttaaga aaagacagct ttagcttgaa attttctcct gctttaactt cttcaggttc
     1081 aaatgagtac atgtttatta taatcttggg aacagttttt tcttccttat ccttatcctt
     1141 atcctcatcc tcttcttcct ttttttctac atacacacct acatatcttg ttaatgtaag
     1201 tttctctatt ttatccttct tgttttcctt atcttccgtt acattttcat attccacgtt
     1261 aatagcaatc aagtaatttt tcgttattgc acttaaagat gcaaacagat taaatttcaa
     1321 ctctttagat tgaccttttt ccaaagcatc tatctggatt acgctgctgg atttaggcaa
     1381 aataccatca tcacatgtta aggaaacctt tacattttgt gccgaatcag tccctacact
     1441 atctaagata aaaccaagat taaaatcatc acctgcttga attttaccag tcgggaaatc
     1501 cagatctttt aatgcaatct ccggttttgt taaatcttcc tcttcttcct ttatttcatt
     1561 gatgcgtaca aaaaactgaa attcatcaga ataagtgttc cctaatccat ccttatattc
     1621 tatatctgct ttaagagaag cactgttacc agtaaacttt tcagatataa ttagaaaata
     1681 ttctgctttc ccctttccct gtccatcaat ttgggaaata tatttactat cagcagattt
     1741 ataagtagtt attccatcaa tacctaaacc cttcaatgta actttaatgt ctttagccgg
     1801 taaagaaccc tggtttttaa gggatagtgc caggctcata atattcccgg gtgaaggatc
     1861 gctaccataa gtctgaatgc tttccataat aagttttggt ggagttttat catttatcac
     1921 ctttatatat atggtctcac cagatgaaaa tgtattgtta taaatatttg agtattgata
     1981 attaagtttt ataggataaa tcccctcttt tgcatcagct tttatcttaa tattatacga
     2041 tattttatct tcttttttag ataatatttt gtctatataa ctgtctagaa taatttgatc
     2101 aaaagtaaaa ggcaagatac tttcattact tgtgtcgata gcagcaataa tatttctcga
     2161 ttcgtacaaa ctgtcattaa ctacaggaag gcttaaaata acatcctcac cggccttaaa
     2221 tgcaggcatt ttaatatcac tttttacttt gatatttggt acagtctttt cagtagtgac
     2281 aggaggagta ttgccgctgc tgtcagattt aaaaaataca attctactat cactatcatt
     2341 tttagcctga ttccattctt cgccttccaa cctgcaccga cagccaatag taataattgg
     2401 ggtatcttca cttccattgt acactagttt aatagtataa ctcctttgtt gttcgtttgg
     2461 gagctccaca ttcttataaa gaaattttcg atctgacgta gcattaatag gcattaatcc
     2521 ttcatttgct cctataatca gttcaatatc agtaattgct ttccctgtat tattatgtag
     2581 agtcacattt acagcaaata cttctcctat aggaagcaca ttaggcattt cctcaacaac
     2641 cagcctaacc ccattatctt cagcttttac agttatacca gctggtacaa ttactgctaa
     2701 aagttgaaag ataattaaaa aacatgccaa aacaacccta gttaaactaa agccatttat
     2761 ttttttattc atggtactat ttaacttttt acaaatacaa ctacttaact tcttcataga
     2821 atcctcccta attttttgcg ttgattgttt caattttctc aacccttcca tccttaatat
     2881 agaatatcct atctgcatat tttgcaatgt cgatatcatg agtaacaatt atcaaagtct
     2941 gattattttt tcttgtcatt gaaataataa gatccataac ttcttttgtt gtctttgaat
     3001 caaggtttcc tgtaggctca tcggcaaaaa caatttccgg gttgctaaca aaagccctcg
     3061 ctattcctac tctttgttgc tgaccaccac tcatctgggt cggtttgtgc ttaaggtggg
     3121 ttcccaatcc tactcctttt agtacttttc tagcctgttt atcccttata gttctgggta
     3181 ttcccttgaa cgtcaaggga atactcacat tttccaaggc agttaaagta gacataagat
     3241 tataagactg aaaaacaaag cccacattct cctgtcgaaa ctttgtcaat tttctttcgt
     3301 ttaatttatg tatttccaag ttttttataa tgatgctgcc ttttgtaggc ttctccaaac
     3361 ctgccataag gtttagcaga gtagactttc ctgaaccgga cgtaccaaga aaacagcaac
     3421 attcaccttt tgatatattt aagctaatac tgtctaatgc tataactttt tcatttccta
     3481 ctctatatac tttccttaca tcctgaagca ctattatatt ctccaaatac tcccacaccc
     3541 ccttaaaaag tattatatca aaattttact tctatatcgt taaaaattta ttaaaatagg
     3601 gaatctatca tcaatagatt tagacgtaca caaattagat aaagtttcag ctttaggaat
     3661 tttttcataa gtctgtcttt tcccaatatg tatttcataa attaagaaga atttaaggag
     3721 aagatattct cttctcctta aagctatata caattcttta ttccttctaa ttattttgaa
     3781 attgtagctt tgtcatgcgg ctttgattat attcttcact aattaactag tgggaacgac
     3841 atgataaaat tgtaacaaga ggttggcttt ttcatcgaaa ctcttcatta catatttgct
     3901 tttttgcgtt tatatagctt tcatatgtat aactatcaaa gcaaacaata aaaacttttg
     3961 aaatattatt atcctcatca gcttcaagaa aattatttat ttcatttacc gcaattcttg
     4021 cagccctatt taccgggaaa ccataagctc ctgtgctaat tgaaggaaag gctattactt
     4081 taatattatt atctaccgca agcttcaggg agtttttgta gcacagggct agaatgtcgt
     4141 cctccctccc atccccgcct ttccataccg gacctacagt atgtattata tattttaccg
     4201 gaagattata accctttgtt atttttgctt cccctgtttc acatcccttt aactttttac
     4261 attcttcaag gagttcgggt cctgcggctc tatgaatagc accatctatt ccgccaccac
     4321 ccaacagggt tttatttgca gcattcacga ttgcatctac ttcaattttt gttatatctc
     4381 cttcaattat tattaacttt tgcataacat cctccataaa tcaaattaat tttgcatatt
     4441 actttagtat attattttag tatattattt tagtatggtt ttatatgcac tctgtataaa
     4501 ctctacagta taatataaaa tattatactg taatcttttt aattagtcca attatagcta
     4561 atataacagg ttgatgaata atataaacag gcaaagtatt tcttcccaac cagcttatta
     4621 tattattcct taattggaaa ttaaaaatgc tccttttatc tttatatagc acttttccta
     4681 atataattcc gtaaataaaa atgccaagcc agggtataag cggatagtag tctgatgatg
     4741 taaatttttc acttgtaatt cccactataa acaaataatc aaaacccgga ttcaaccttg
     4801 atacataacc tcccaatatt agtataacag tacctgacat cataagtaag tattcattga
     4861 gattatttag caatggatat gttaatatgc tcaatccaag aaaatgcaat atcccaaact
     4921 ttattcccat atcaacatta taaagatgtg taagtacagt tataatcata gctatggcaa
     4981 gtattttcaa ggctcttttc atattgtttt tacttaaagt acaacttatg ccggaaatca
     5041 atataaaaag tattcctgca gccctccctg ttaaataatt gaacccctta ctataatata
     5101 cattatatcc gaaaatatct ttcatgtcaa aaattatatg gaaatatatc attagcagta
     5161 acgcaattcc ccttaataag tccaactccc atatacggct tttttgttta atgccttcct
     5221 taatattgtt ttgcaggttt ttttcactac tattgacatg taaatttact tcatcctcca
     5281 tatattcccc ctctttacca aataaacatc ttctaaccta atttatctca atataatatg
     5341 gctatccaac cttcttgtga aggctgatga aattcaaggt accttaaatt tcatcagaaa
     5401 gtcatttcat gactttctgg gtgataacca tataatataa ctaaacatgc tgtacgtcaa
     5461 cttaagtaga aatattgcgg taaaagaatt cattatttgt tttgtgaaat tttaatattt
     5521 tttgtaagaa acaaaccttt agatgaaaat tattgttaca gtaggtaatt tagtttataa
     5581 tgaaataaat gtataatata attgcataga agcaaaaaga agcagaagat gtatagaaac
     5641 aaaaagaagc aaaaaaatcg tccccatgct tcacatgctt ccaaaaatat taaaaaagcg
     5701 gagtggttat aatgataatt atcggagaaa aaatcaacag tactctcaaa gctgtgcggc
     5761 cggctattga aaactatgat atcgggttta tacaggagct tgccaaaaag caatttgaag
     5821 caggagcgaa ttttattgat gttaatgcag gtatgtttca tgatgatgag ccaaaaagac
     5881 tgaaatggtt ggttgaaaca gttcaggagg taattggcac accatttgcc attgactcgc
     5941 ctaacccaga agctatagaa gaagcactta aggtcaataa aaatgggaaa ccaattatta
     6001 actcaataac agatgaaaaa gaaagatatg aagctatttt acctttgata ctggaatata
     6061 acacaggagt aatagctctt tgcatggacg acagtggcat gcctgaaact gtggatgata
     6121 ggttggaggt tgctgtcagg ataattggta aattaacaga caaaggagta aagccccagg
     6181 acatttatat tgatcctatg ataagaccta taggcacagg ctctcattat ggtgttgtag
     6241 cactggaaac aataagacag gtaaaggata agtactctga tgttcatata gcttgcggtc
     6301 ttagcaatat ttcctttgga cttccgggaa gaaaagtatt aaaccaggca tttcttatag
     6361 ctgcaatgac agtaggcatg gatggagcaa tattagatcc tcttgataaa aaacttatga
     6421 cttaccttta tgctacccaa gcccttttag ggcaggatga attttgcatg gattatttat
     6481 caaaatatag agaaggactt cttgatttgt aaagaatatg agtaatatga atgtattagg
     6541 ggtaattgct gaatataatc cctttcataa tggacatctt tatcatttac aggaatctat
     6601 aaaatgtact tctgcaaatt tcatagtatg cgctatgagt ggtaattttg tccagagggg
     6661 tgaacctgct attataaaca aatgggcaag aactgaaat
//
LOCUS       DAOSDD010000232         6615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4205_length_6615_cov_5.104116, whole genome shotgun sequence.
ACCESSION   DAOSDD010000232 DAOSDD010000000
VERSION     DAOSDD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_6615_cov_5.104116"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PK733_16010"
     CDS             <1..1737
                     /locus_tag="PK733_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOJ12077.1"
                     /translation="VYSNRIREAYGNVPIILGGLEASLRRFAHYDYWSNKIRRSILLD
                     AKADLIVYGMGECQIVEIAEALESGIPISEITFIKGTVYKTKDRERAYNPVFLPSYEE
                     ILASRRKYAESFMIQYNNMDAITSKPLIETYSNNNYFVVQNPPREPMKQSELDRVYSL
                     PYMRNYHPIYEKSGGIPAIEEVKFSLISNRGCFGGCNFCALTFHQGRVVQSRSHQSII
                     SEAEKLIWDPDFKGYIHDVGGPTANFRHRACEKQEKYGVCINKQCMFPNPCKQLKIDH
                     TDYLKLLRSLRRLPNVKKVFVRSGIRYDYLIYDKNDEFFRELCKYHISGQLKIAPEHV
                     SVEVLNKMGKPQKEVYERFVKKYNKINEELGMNQFLVPYFMSSHPGSTLKDAVELAEY
                     LRDLGYMPEQVQDFYPTPGTLSTCMFYTELDPRTMEKVYVPKSDHEKAMQRALIQYGK
                     PQNYKLVFEALQKAGRTDLIGYDKKCLIKPKSYKHNVESEKSKDTTRAGSKRGAKGRY
                     KAKLREGSKTRSKDEAKDKAITKLKNWTKDRVIAKSGDGTKAKVTVEPEGKSKMNNKR
                     VKSNRIRSNKNK"
     gene            1910..3589
                     /locus_tag="PK733_16015"
     CDS             1910..3589
                     /locus_tag="PK733_16015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ12078.1"
                     /translation="MRKIILFISIITCVTLLVLFISVNIVNQNEELYTSAIPPEIELT
                     DGTNSIKGSVKMDSWYYKGKDNKFYPYPNNSAKNDNFQNGNAQNNSVKSDSAKIGNSQ
                     SVSAQKDSIQNFNAINGNFRTFENPDSSINLTNDPAVLSYRGDLALKFSPYSDQYSQQ
                     YPDQDPDKCPDKFNLQIFHKGNLIFQENDVVSEFHYPVYQPKEDGELLYKITAFWYES
                     ESASRGFYGQVTYEFAFYNDVPIEFVVSPTKTFPGEMVTVSVKYANENEKISLKSDLL
                     KSEIPFYKNEHGQIAVLPLSYDLQPSEYVINLEVQKTNDSSSESPDYKDIRLNESDNK
                     ESDTRDTKKDEVKNPQGTSDMDSLVTENVNLAGKKEFQKITISVQPKDFPIQYLTISD
                     DVDKSTRNDAAYEEYDKYIGAVRKTDTPVKMWDGVFLKPVEGRITTEFGMRRFVNDAP
                     TSYRHSGIDIAADRGTPVKATNSGKVILARHLILIGNTVLIDHGYGIISWNYHMDSIA
                     VKEGDDLVKGQIIGKVGSTGFSTGPHLHFAISVHDIFTNPWTLFEQEPEGL"
     gene            complement(3608..3823)
                     /locus_tag="PK733_16020"
     CDS             complement(3608..3823)
                     /locus_tag="PK733_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12079.1"
                     /translation="MLTRKEYIFPMLSKIIPEDIAFQISYSSIVLSSLSYHISEVNSN
                     PTSRAHSLSMIQNMALYDFPSINLSKA"
     gene            3901..4779
                     /locus_tag="PK733_16025"
     CDS             3901..4779
                     /locus_tag="PK733_16025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12080.1"
                     /translation="MGKIDIHVHARIKRVTNFMGTDTSYATAEELLGMYEKLGIEKGV
                     ILPEVNPECATCIQSNEEVMEIVNKYPGRFDWFCNIDPRMGGNDSNTDLSYYLTYYKK
                     LGAKGVGELCCNLYFDDPMVENLFYHCERMEMPVIFHIGPQIGGCYGLVDEQGLNRLE
                     KELAKFPKLKFIGHSQPFWAEIGAGVDNKSRQGYPKGKVIPGRLVELMRKYPNLYGDL
                     SADSGYNALVRDPEFGCRFMEEFKDRLLFGTDICSPKNDMQLSFWLDAAVRDNKMSSD
                     IYEKICRKNALEILGH"
     gene            5092..5844
                     /locus_tag="PK733_16030"
     CDS             5092..5844
                     /locus_tag="PK733_16030"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12081.1"
                     /translation="MKIIDINTMIGPWPSRLHRFNDEVSLIKEMDSYRITSAITYSSI
                     ALNNPKEGNKHIKDIAEASNDRIKACYILDSNIGSSEIPDASELMKQLKEEKPSAVKL
                     YPNRNKYYLDSFYCGELLEVLDVLNMPVIIDSDQITSFKDLPQLAKSYSNIKFIILRY
                     GFRESRTVIPLVKKLDNVYFDISVMVDTGLIEEIVNKYGSDKLLFGSGLPFYVPAGSL
                     GMVIYARLKNSDKEKIFTGNWEYVEGGINYGD"
     gene            5834..>6351
                     /locus_tag="PK733_16035"
     CDS             5834..>6351
                     /locus_tag="PK733_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12082.1"
                     /translation="METKVINNIKSGYNISNIKNGLNARNNSCIYSDPIREEAFAGKP
                     VSVPVIDSHSHICAYYNSGWYQSFTSNKEVISLMEHLGIDCIITAPHILVAGRMEDAN
                     KAADRAAKEFPGRIYGYISICPHEGINAIKDAINNYSKNSSFIGMKFLPGYHGPLACI
                     EYDYALDFANEVG"
     assembly_gap    6352..6361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6362..>6615
                     /locus_tag="PK733_16040"
     CDS             <6362..>6615
                     /locus_tag="PK733_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12083.1"
                     /translation="GCPVLIHTWSGSPGLNEFERVVKTRSNLKLMVAHQGGGNGKCTD
                     DLAALMKQYPNLYMEICGSLFNQYSIEDMVGMAGEDRVIYG"
BASE COUNT         2305 a         1029 c         1356 g         1915 t
ORIGIN      
        1 gtatattcca atagaattag agaagcatat ggcaatgtac caattatatt aggtggattg
       61 gaggcaagtc tgcggagatt tgcccactac gactattggt ccaacaagat tagaagatcc
      121 attcttttag atgcgaaagc tgatttgatt gtctatggaa tgggagaatg tcagatagta
      181 gaaatagcag aagcattgga aagcggaata ccaataagtg aaatcacatt tataaaggga
      241 acagtttaca aaaccaaaga cagggaaaga gcttacaacc ctgtattttt accttcttat
      301 gaggaaatac tggcatccag gagaaaatat gcagagagtt ttatgataca atataacaac
      361 atggatgcca taacaagcaa gcccctgata gagacctaca gtaataataa ttattttgtg
      421 gtgcaaaatc ctcctcgtga gcccatgaaa caatccgaat tggatagggt atattcactt
      481 ccatatatga gaaattacca tcccatatat gaaaaatctg gagggattcc ggccatagag
      541 gaggtaaaat tcagtctcat aagtaacaga ggctgttttg gcggctgcaa cttctgtgct
      601 cttaccttcc atcaaggcag ggtagtgcag tcaagaagtc atcaatcaat tatatcggaa
      661 gcagaaaaac taatatggga tcctgatttt aaaggttata ttcatgatgt gggaggcccc
      721 actgcaaatt ttcgccatag agcctgtgaa aagcaggaaa agtatggggt ttgcataaat
      781 aagcagtgta tgtttccaaa cccttgcaaa cagcttaaga tagatcatac agactacttg
      841 aagcttttga gaagtcttag gagacttcca aatgtaaaaa aggtatttgt gagatcagga
      901 attagatatg actaccttat ttatgataaa aacgatgaat ttttcagaga actgtgtaaa
      961 taccacataa gcggtcagtt aaagatagcc cctgaacacg tatctgtcga agttctgaat
     1021 aaaatgggca agccgcagaa agaagtatat gagcgatttg tgaagaaata taataaaatt
     1081 aatgaagaat tgggcatgaa tcaatttctt gttccatatt ttatgtcaag tcatccggga
     1141 tctacactga aagatgccgt tgaactggca gagtacttaa gagatttagg gtatatgccc
     1201 gaacaggttc aggactttta tccaacaccc ggaacattat caacatgcat gttttatact
     1261 gaactagacc caaggaccat ggaaaaagta tatgtgccaa aatcagatca tgaaaaggcc
     1321 atgcaaagag ctttgattca atacggcaaa ccacagaatt ataaattggt atttgaagct
     1381 ctccaaaagg cgggcagaac cgatttgatt ggatatgata aaaagtgttt gataaagccc
     1441 aaatcttaca agcataatgt tgagtccgaa aagtcgaaag atacgacaag agccggatct
     1501 aagagaggag caaaaggcag atataaagcc aagttgaggg aaggatcaaa aacaagatca
     1561 aaggatgagg cgaaagataa agctataacc aagctgaaga attggacaaa agatagagtt
     1621 atagctaaat cgggagatgg tacaaaagct aaggttacag ttgagccgga aggcaaatca
     1681 aaaatgaata acaaaagagt taaaagtaat aggataagaa gcaacaaaaa taaataaact
     1741 gtatcattag cgaaataaac caccaacgaa ctgcatcacc gttaaggcgc aatgagctgc
     1801 tgactctcca aaaaactggt gcaccagcag cgtgcccaac atttctactg ctgggtagga
     1861 actattttgc atagtaaaag gttaggatat gtgtacatgg aggttaatta tgagaaaaat
     1921 tattttattt atttccataa ttacttgtgt aactttattg gttttgttta tttctgttaa
     1981 tattgttaat cagaatgagg aattatacac atctgccata ccccctgaaa ttgaattgac
     2041 agatggaact aattccataa aaggttcggt aaaaatggac agttggtatt ataagggaaa
     2101 ggataacaag ttttatccct atccaaataa cagtgccaaa aatgacaatt tccaaaatgg
     2161 taatgcacaa aataatagtg taaaaagcga tagcgctaaa atcggtaatt ctcaaagcgt
     2221 tagtgcccaa aaagatagta tccaaaactt taatgctatt aatggcaatt ttagaacttt
     2281 tgaaaacccg gattcctcaa ttaatttaac caatgacccg gcagtactct cttatagagg
     2341 agatttagct cttaaattca gcccatattc tgaccaatat tctcaacaat atcccgacca
     2401 agatcctgac aaatgtcccg acaaattcaa cctgcaaata ttccataaag gcaatctgat
     2461 ttttcaggaa aatgatgttg tttctgaatt tcattatccc gtttaccagc cgaaagaaga
     2521 tggggagtta ctttataaaa tcaccgcttt ttggtatgaa tcagaatcag ccagcagagg
     2581 cttttatgga caggtaacat atgaattcgc tttttataat gatgttccta ttgaatttgt
     2641 ggtttctcct actaaaactt ttcctggaga gatggtaaca gtttctgtaa aatatgcaaa
     2701 cgaaaatgaa aaaatttctt taaaatctga tttgttaaaa tcagaaatac ctttttataa
     2761 aaacgaacat ggacagattg ctgttcttcc attaagttac gatttgcagc cttctgaata
     2821 tgtaattaat ttagaggttc aaaaaacaaa cgattcgagc agcgaaagcc cggattataa
     2881 agatattagg ttaaatgaat cagataacaa agagtcagat actcgagaca ctaaaaaaga
     2941 tgaggttaaa aatcctcaag ggactagtga tatggattct ctagttacag aaaacgttaa
     3001 cctggcaggc aaaaaagagt ttcaaaagat aaccattagt gtccagccca aagatttccc
     3061 tatccaatat cttactattt ctgatgatgt ggataaatct acaaggaatg atgctgctta
     3121 tgaagaatat gacaaatata taggagctgt aagaaaaact gatactcccg ttaaaatgtg
     3181 ggacggagtt ttcctgaagc ctgttgaagg ccggatcacc actgaatttg gtatgagaag
     3241 atttgtgaac gatgctccga cttcatatag acatagtggc attgatattg ctgcagatag
     3301 aggtacacct gtcaaagcaa caaattctgg taaagtaata cttgcaagac acctgattct
     3361 tataggtaat actgttctta tagatcatgg atatggaata attagctgga actatcatat
     3421 ggacagtata gcagttaaag aaggtgatga tttagttaaa gggcaaatta taggcaaggt
     3481 tggttctaca ggcttttcca ccggcccgca tcttcatttt gcaatatccg tacatgatat
     3541 ttttacaaat ccctggacac tttttgaaca ggaaccggaa gggctatgaa ggaaaatatg
     3601 gcaatcccta agctttgctc aaatttatac tcgggaaatc atatagtgcc atattttgta
     3661 tcatagaaag agaatgtgcc ctgcttgttg gattgctatt aacttctgag atatgatatg
     3721 ataagcttga taaaacaata ctactatagc taatttggaa agcgatgtct tccgggataa
     3781 tttttgatag catcgggaag atgtattcct tacgagtcag caaaaattat cccccagaaa
     3841 tatgctctcc attggcaaca taacaaagta gtactaaatt aaaaaatgaa ggtgatacac
     3901 atgggaaaaa tagatattca tgttcacgca aggataaaaa gggttacaaa ttttatggga
     3961 acagatactt cttatgcaac tgctgaggag cttcttggca tgtatgaaaa acttggcatt
     4021 gaaaaaggtg ttattttacc cgaggtaaac ccggaatgtg caacctgtat acagtcaaat
     4081 gaagaagtta tggaaattgt taataaatat ccgggtagat ttgactggtt ttgcaatatt
     4141 gatcctcgta tggggggaaa tgattcaaat accgacttgt cttactattt gacatactat
     4201 aaaaaacttg gtgcaaaagg tgttggtgaa ctttgttgca acctttattt tgatgacccc
     4261 atggtggaaa acttgtttta ccattgcgaa aggatggaaa tgccggtgat tttccacatt
     4321 ggtcctcaaa ttggaggctg ctatggactg gtagatgaac agggtttgaa ccgattggag
     4381 aaggagctgg ccaagtttcc aaagcttaag tttataggtc attcccagcc attttgggca
     4441 gagataggtg ctggtgttga taataaaagc aggcaggggt atccaaaagg gaaagtcatt
     4501 ccaggcaggt tggttgaact tatgaggaag tatccaaatc tttatggtga cctttctgca
     4561 gacagcggct ataatgctct tgtgcgtgat cctgaatttg gatgcaggtt tatggaagaa
     4621 tttaaagacc gtttgctttt tggaacagat atatgttctc ctaaaaatga tatgcaactt
     4681 tctttctggc ttgatgctgc agtaagggat aacaaaatgt ccagtgatat atatgaaaag
     4741 atttgcagga aaaatgcact tgagatcttg ggacactaaa atgattcttc ataatgttga
     4801 gttatttgaa agtgaatctt tgaaattatt ttatgagtac aattagtaaa taatagggtg
     4861 catttaaaat ttttgaagaa atatattcca gaagctatcg ttacgttgct tagagccttc
     4921 actaatgctt cttccaataa tttcttcaaa aataaaagat gcacccttaa atattattta
     4981 tccaaaaagt gtgcttaaat caaaaagcag ttgaattaaa aaacgagttg gtgtaatata
     5041 gtattaactt gtaaaacgtt ttagtagaaa tttttactgg gggtgaattt attgaagata
     5101 atagatataa acacaatgat aggaccatgg ccttccagac ttcatcggtt taatgatgaa
     5161 gttagcttaa taaaggaaat ggatagctac agaataactt ctgctataac ctattcgtct
     5221 attgcattga ataacccaaa ggaaggcaat aagcacataa aggatattgc tgaggcttcc
     5281 aatgatcgta ttaaagcttg ttatatactt gatagtaata ttggttcttc ggaaatacca
     5341 gatgcttcag aacttatgaa acagctgaaa gaggaaaaac catctgcagt aaagctatat
     5401 ccaaacagaa ataaatatta tcttgatagc ttctattgtg gagagttatt agaagtgtta
     5461 gatgtgttaa atatgcctgt gataattgat agtgatcaaa ttacttcttt taaagacctg
     5521 ccgcaattag ctaaatcata ttcaaatatt aaatttatta ttctgcgata tggtttccgt
     5581 gaatccagaa cagtaatacc gctagtgaaa aaacttgata atgtatattt tgatataagt
     5641 gttatggtcg atacgggatt aattgaagaa attgttaaca agtatggaag cgataaactt
     5701 ctatttggaa gtggattacc tttctatgtt ccagcaggtt ccctgggtat ggttatatat
     5761 gcaagactaa aaaatagtga taaagagaaa atctttactg gaaattggga gtatgtggag
     5821 gggggcatca attatggaga ctaaagtaat caataatatt aaaagtggct ataatatcag
     5881 taatattaaa aatggcttaa atgctagaaa taactcatgt atttacagcg acccgatacg
     5941 cgaagaagcc tttgctggaa agccagtatc tgtcccggtt atagattccc attcccatat
     6001 atgtgcatat tataacagcg gctggtatca atcttttaca tcaaacaaag aagttatatc
     6061 attaatggag catttgggta tagattgcat tataactgct ccccatattc ttgttgcagg
     6121 caggatggag gatgcgaata aagcagctga tagggctgca aaagaatttc caggtaggat
     6181 ctatggttat atttccatat gtcctcatga aggtattaat gctattaaag acgcaataaa
     6241 caactatagt aaaaacagca gctttattgg tatgaaattt ctacctgggt accatggtcc
     6301 gcttgcatgt attgaatatg attatgcttt ggattttgcc aatgaagttg gnnnnnnnnn
     6361 nggttgtcca gttctaatac atacttggtc aggaagcccg ggattaaatg aatttgagag
     6421 agtagtcaaa acaagatcca atctaaaact tatggtagct catcaaggtg gcggaaatgg
     6481 gaagtgtact gatgatttgg ctgctttaat gaaacaatac cctaaccttt acatggagat
     6541 atgtgggtca ttatttaacc aatattctat tgaagatatg gttggaatgg ctggagaaga
     6601 tagagtaata tatgg
//
LOCUS       DAOSDD010000233         6581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4228_length_6581_cov_4.715446, whole genome shotgun sequence.
ACCESSION   DAOSDD010000233 DAOSDD010000000
VERSION     DAOSDD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_6581_cov_4.715446"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK733_16045"
     CDS             complement(<1..627)
                     /locus_tag="PK733_16045"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOJ12084.1"
                     /translation="MNYTTQMDAARKGLITKEMKFVAEKENVDPEILKRYVAEGKVAI
                     PSNKNHKALNPEGVGQGLRTKINVNLGVSKDCCSMEEELKKVEKAIEMKAEAIMDLSS
                     YGKTEEFRRRLIDMSSAMIGTVPVYDAVGFYDKDLENITSKEFIDVIEKHARNGVDFM
                     TIHAGINRETAEVFKRNQRQLNIVSRGGSLIYAWMELNNKENPFFEYFD"
     regulatory      complement(963..1062)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1163..1885)
                     /locus_tag="PK733_16050"
     CDS             complement(1163..1885)
                     /locus_tag="PK733_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOJ12085.1"
                     /translation="MKNKTGEKTQAKGSLLEKDFKKQPETLAQKIKSKLTKDKIDYFD
                     MLVKGAAISLEAAKNLKAAFADSEINKEEIRRIKEVEHKGDKHMHESQRIIEAAFITP
                     IDQGDIMGILKGIENITDSIDDIANHIYIMRVEKSDEFMSRFVQIMISSCEKTYALMD
                     ALKQFRKNPNKNINTLIIEINALEEEGDRIYSESMRHLFGFETDPVNIIKKKEIYQCL
                     EHTLDCCEDVADMVENLMIAKS"
     gene            complement(1927..2931)
                     /locus_tag="PK733_16055"
     CDS             complement(1927..2931)
                     /locus_tag="PK733_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOJ12086.1"
                     /translation="MYVILVTVVILVCCSIFVNGWTDAPNAIATVISTRVLNPRAAIV
                     LGTVFNLLGVFLMGSAVATTTASIVTIGTGKDALVTLGAAQLSIIIWAVSAWRYGIPT
                     SESHALIAGLMGAGISLNGINAFNWDSFKKVLIGLVISSVVGFGLGLFSTKSIEVLFK
                     NVKRRSANKFFSRGQILSASLMAFSHGAQDGQKFMGVLYLALVIGGVYSNNASGNFDI
                     PIWIMILCSLLMGVGTSVGGYRIIKTMGMDMVRLEKYQGFAAEVVASGSMIAATLFGI
                     PLSTTNTKATAMMGAGASKGFNRVNWGIAKEMILAWILTFPVCMALGYVFATVFRMIL
                     "
     gene            complement(3002..3343)
                     /locus_tag="PK733_16060"
     CDS             complement(3002..3343)
                     /locus_tag="PK733_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12087.1"
                     /translation="MLAIEKKDYHNKQSFCRQLNTYLTGFLSEEPDWLAGLSNASALL
                     YLLLPRINWAGFYIYKGGELVLGPFQGKPACTCIALGKGVCGTVANDRQIQLVDDVNQ
                     FPGHIACDAAS"
     gene            3783..4250
                     /locus_tag="PK733_16065"
     CDS             3783..4250
                     /locus_tag="PK733_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12088.1"
                     /translation="MQRLNWIELVFGVVPESLIFIFGIYIFSNTLICKKRLFLSALII
                     SISTYIIKLLPIQFGVHMLILIMALVGVSSFINHVEIIKAVTSALLLIIIRLITEWIS
                     LLIMRELLNISIDVFLDNPLIKVLYVYPSTFMFFLVVFIVYIFKKKNCKERVI"
     gene            4252..4923
                     /locus_tag="PK733_16070"
     CDS             4252..4923
                     /locus_tag="PK733_16070"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HOJ12089.1"
                     /translation="MYVLENFSNVLASKIALYLNLDKEREEVLAYGAFGFIHTLWSTL
                     LLVMFGVLFQSLPIILIIAFTVAALRKFSGGAHAASPNRCAAISVLVFGTLSLIIKYA
                     AIYANILSVILYQTASFVFTYVILYKYCPVDTPNKPVKKQELRQKLRKASFISVFCYF
                     CITVFLWAGFLKTNASFMRASIISICTGMLWQSITLTHVGHFIIEKLDTILQNINFMK
                     GGRKK"
     gene            4920..5057
                     /locus_tag="PK733_16075"
     CDS             4920..5057
                     /locus_tag="PK733_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HOJ12090.1"
                     /translation="MKKKVLMTVFAIGAALLTFLASASAASACWFFMYQPQEPKSLRK
                     E"
     gene            5235..>6581
                     /locus_tag="PK733_16080"
     CDS             5235..>6581
                     /locus_tag="PK733_16080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ12091.1"
                     /translation="MFDIDNDRQKKLYEIIAIMKLFSLLVSGMLVFSKYLIFSNAADA
                     YAVFTSFSAITLFTLLCFFIYEIWVFSNKRKGRSFSLLNILEVSIFVAIFSTLVILSE
                     STDTHAKLLFLFVIITSSIEFGIRFGTIIASISSAIILMLDLIYIPYSVINITFQNDL
                     TLVGVFILTAWLLGYYEKIEREHREKMTYMAIIDGLTDIYNHRFFQESLRNHIAKAQA
                     MGYSVSLALVDIDCFKYYNDLYGHQAGDNILKEIALILKNNVRPCDIVARYGGEEFVI
                     IMPETTETQALEIAEHIRTIVDKTAFEGEENLPNANLTISVGVSCYPEKAKNLQELIN
                     STDDALYRAKFFSKNRVEPYRSVLEELKKDIDDKHFDLVASIKTLISVINAKDRYTYA
                     HTERVVIFCELIAQQLNLSAEDSKILKYGAYIHDIGKIHISEDILNKKMPLNDNEWN"
BASE COUNT         2096 a         1213 c         1010 g         2262 t
ORIGIN      
        1 atcgaagtat tcaaagaaag ggttctcttt attgttcagt tccatccacg cataaatgag
       61 agatcctccc cttgatacta tgttcaattg tcgctgattt ctcttaaaaa cctcagctgt
      121 ttcacgattt attcctgcat gaatggtcat aaaatctact ccattccttg catgtttttc
      181 aataacatca ataaattcct ttgaagttat attctccaaa tccttgtcat aaaaacccac
      241 agcatcatat acaggaaccg tacctatcat ggcagaagac atatcaataa gtcttcgtct
      301 gaattcttca gtttttccat aggaactgag atccattata gcctcagctt tcatttcaat
      361 tgctttttcc acctttttaa gttcttcctc catactgcag caatcttttg aaacaccaag
      421 gtttacattt atttttgtcc ttaagccctg tccgactcct tccggattaa gagccttatg
      481 gtttttgttt gaaggtatgg ctaccttccc ttcagccaca tatcttttca gtatttccgg
      541 atccacattt tctttttcag ctacaaactt catttctttt gtaattaagc ccttcctggc
      601 agcatccatt tgagtagtat aattcatatt ccttctcctt ttctttaaat aagtatttaa
      661 tatgtgatgt gcccaatgta tttatcagtt atctcattta tagcaaactt cagcagttct
      721 aataaatata acaactttat cattggatac atattaacaa tgtaatacca gggatatctt
      781 gattgagttt atcatttaag gaagactatt ttacaatgtg ctttaatgca cttaatgaaa
      841 agtttacatt aaggtaattt atactttcct attcgttaca aaaaatgctt acctaataag
      901 gtaagcattt aattatcgat ctaatttaac caaattttat ctaagtaaaa ctactattat
      961 tttagtaatt ttactctgct tccctacggt agtattagct acatcaggtt caaagggtca
     1021 ggttctacct tctcaaccaa aatggtcccc ccacaaatct atattatcaa tttgccaagt
     1081 gtattaagtt gttactttaa ttatacacaa aatagaattt gctgtaaagc atcaaaaaag
     1141 gaaagggaaa tggtgcatat tttcacgact tcgcaatcat taagttttcc accatatctg
     1201 caacatcttc gcaacaatct agtgtatgct caagacactg atatatttct tttttcttaa
     1261 taatatttac cggatcagtt tcaaagccaa aaagatgacg catgctttca gagtaaatac
     1321 ggtctccttc ttcctccaat gcattgattt ctataatcaa agtattaatg ttcttatttg
     1381 gatttttcct gaattgtttt aacgcatcca tcagagcgta ggttttttca catgaggaaa
     1441 tcattatttg aacaaagcgc gacataaatt catcactctt ctctaccctc attatataga
     1501 tatgattggc aatatcatca atactgtctg tgatgttttc tataccctta aggataccca
     1561 tgatatcacc ctgatcgatt ggagtaatga acgcagcctc tatgatcctc tgggattcgt
     1621 gcatatgctt gtccccttta tgctctactt ccttaatacg gcgaatctct tctttattaa
     1681 tttcactatc tgcaaaagca gccttgagat tttttgcagc ttctaatgaa atagctgctc
     1741 cctttactaa catatcaaaa taatcaattt tatctttagt taatttgctt ttaatttttt
     1801 gtgccagtgt ttcaggttgc ttcttaaaat ctttttccaa taagctccct ttagcttgtg
     1861 ttttttcacc tgttttgttt ttcataaaat tacctccgtt attgtcacac agtagtattt
     1921 gatattttat aaaatcattc ggaatactgt tgcaaataca tatcctaagg ccatgcaaac
     1981 cggaaatgtc agtatccacg ccaaaatcat ctctttagca attccccaat ttactctgtt
     2041 aaagccttta gaagctccag cacccatcat ggctgttgct ttagtgtttg tagtactcag
     2101 aggtatccca aataaagttg cggcaatcat actgcctgat gctacaacct cagctgcaaa
     2161 gccttgatat ttttccagcc ggaccatatc catgcccatt gtttttataa tacgatatcc
     2221 accaacagaa gttccaacac ccatcagcag tgagcataat atcataatcc aaattggaat
     2281 atcaaaattt ccagacgcat tatttgaata aactccacca ataaccaaag ctaaataaag
     2341 aactcccata aacttttggc catcttgcgc tccatggctg aaagccatta atgaggcact
     2401 taatatttgg cctcgtgaaa aaaacttatt tgctgaacga cgttttacat ttttgaatag
     2461 gacctcaata gatttagttg aaaaaaggcc taacccgaaa cctacaactg aagaaattac
     2521 taaacctatt agcacctttt taaaagaatc ccaattaaat gcgttaatac cattaagaga
     2581 aatccctgcc cccattaatc ccgctatcaa agcatgactt tcactggtgg gaattccata
     2641 ccgccaggcc gatactgccc aaataattat agaaagctga gctgctccca atgtcacaag
     2701 agcatccttt cctgtaccta ttgtaacaat gctggcagta gtagtagcta cagctgaacc
     2761 cataagaaac acacctaaaa ggttaaaaac agtccctaaa acaattgccg cccttggatt
     2821 gaggacacgt gtggatatca cagtagcaat ggcatttgga gcatctgtcc aaccattcac
     2881 aaatattgaa caacaaacga gaataacaac agtaacaagt attacataca taagtcattt
     2941 tccttcctta aatcaaataa ccaacattgt taattctagc ataaaataat ttactagcca
     3001 actatgatgc agcgtcgcat gcaatatgtc cggggaactg gttcacatcg tcaactagct
     3061 gaatttgtct atcatttgca acagtaccgc atactccttt ccccaaggca atacaggtac
     3121 atgcaggctt cccctgaaaa ggtcccagaa caagctcacc acctttatat atataaaatc
     3181 ctgcccaatt aatacgggga agtaataagt ataataaagc agaggcattg gaaagacctg
     3241 caagccaatc aggttcctca cttaaaaatc cggtaagata agtgtttaat tgtctgcaaa
     3301 atgattgttt attatgataa tctttctttt caattgctag catgcttttt cccctttcat
     3361 tttttagtat gagttatatg tcattagcct taccatttct gacattcaaa gaaacattac
     3421 gtgatttttc atctacaaca tatagtttta cactcctgag tttattttga gatttaaacc
     3481 ctatacttat gtatataatt tatgtatata attttattga tgctatgtca agtatatcaa
     3541 gggtgcattt aatttatctt aagaaaatat tggcaaggag cattagtgaa ggtgctgtag
     3601 ctaggcaggg gaaactcttc tctcaagtaa caggtattcc tttttgactc actttgacaa
     3661 agcaaaaaca aagtaatata ataacttgaa atatattttt tataataaaa aaaagggttt
     3721 acaaaagtta tgtcgaataa gtaaatatta attattttat tgtattgggg atgtagactt
     3781 atatgcaaag actaaattgg attgaattag tatttggtgt tgttccggaa agccttatat
     3841 ttatttttgg aatttacata ttttccaata cattgatatg caaaaagaga ctctttcttt
     3901 ctgcattaat tataagtatt agtacataca taataaagct gttacccata caatttggtg
     3961 tacatatgct tatcttaatt atggcattag taggagtatc ctcctttata aatcatgttg
     4021 aaattataaa agcagttacc tcagctttgt tgttaattat tatccgcttg ataacagaat
     4081 ggattagcct attgataatg agagagttat taaatatatc aattgatgtt ttcttggata
     4141 atccactaat aaaagtttta tatgtatatc catcaacttt tatgttcttt ttagtagttt
     4201 ttatagtata tatttttaaa aaaaagaatt gtaaggaaag ggtcatttag tatgtatgta
     4261 ttggaaaatt tttctaatgt tttggcttct aaaatagctc tataccttaa tcttgacaag
     4321 gaacgtgaag aagtccttgc atatggtgct tttggtttta tccatacttt atggtcaact
     4381 ttacttttag ttatgtttgg agttctattt caatcattac caataatatt aattattgca
     4441 tttacagtag ctgcattaag gaaattttcc ggaggagctc atgctgcatc ccctaaccgt
     4501 tgtgcagcta tttcagttct tgtatttggg actttgtctc taataataaa gtatgctgcc
     4561 atatatgcca atattttatc tgttatccta tatcagacag catcatttgt ttttacatat
     4621 gttattttat ataaatactg cccagtagat actcccaaca agcccgtaaa aaaacaggag
     4681 ttacggcaaa aactccgtaa agcatctttc atttctgttt tttgctattt ttgtataaca
     4741 gtatttctgt gggcaggttt ccttaaaaca aatgccagct ttatgcgggc aagtataatt
     4801 tctatatgta caggcatgct ctggcagtct attacattaa ctcatgtggg acattttatt
     4861 atagaaaagc ttgatactat cctacaaaat atcaatttta tgaaaggagg tagaaagaaa
     4921 tgaagaaaaa ggttctcatg acagtgtttg ctataggtgc agcattatta acattccttg
     4981 catctgcttc tgcagcttca gcatgctggt tttttatgta ccaacctcag gaaccaaaga
     5041 gcctacgtaa agaataatcc tgagttacct catcatcaag gaaagaatgt tttgaaaaat
     5101 cagtacatat ttagtcaatc tactgatttt ggtgcaggct tgcacctgac agaaataaga
     5161 aaggttgcat acagcctttc ttatttccct attttatatg attttaaata attactattg
     5221 taggtgattt ataaatgttt gatattgata atgacagaca aaaaaaactt tatgaaataa
     5281 tagcaataat gaaattgttt tcattgttag tatcagggat gcttgttttc agcaaatatc
     5341 tcatttttag caatgctgcc gatgcatatg cagtttttac aagtttttcc gcaattacac
     5401 tatttacttt actgtgcttt tttatatatg aaatctgggt gttttccaac aaaaggaaag
     5461 gccgttcttt cagcctatta aatatacttg aagtgtctat ttttgttgct atattttcta
     5521 cacttgtaat tctctctgaa tccactgata cacatgccaa gctgcttttt cttttcgtta
     5581 taattacatc aagtattgaa tttggcatac gttttggtac tattatagct tcaatatcct
     5641 ctgcaataat tttaatgctt gacttaatat acataccata ttctgtaatt aatataactt
     5701 ttcaaaatga tcttactctt gtgggggttt ttatactaac agcctggctg ttagggtatt
     5761 atgagaaaat tgaaagagaa catcgtgaaa aaatgacata tatggcaata atagacggcc
     5821 ttaccgatat ctataatcat aggtttttcc aggaatcatt aaggaaccat atagctaaag
     5881 ctcaggctat gggatattct gtttcactgg ccttggtcga tattgattgc tttaaatact
     5941 ataatgatct atatgggcat caagcaggag acaatatact taaggaaata gccctcattt
     6001 tgaaaaataa tgtaaggcca tgtgatattg tagcccgtta tggtggtgag gaatttgtca
     6061 taattatgcc tgaaactaca gaaacccagg ctcttgaaat tgccgaacac attagaacta
     6121 tagttgataa aacagctttt gaaggtgagg aaaacctgcc taatgctaac ctcaccatat
     6181 cagtaggagt ttcctgttat ccggaaaaag ccaaaaattt gcaagagctt attaacagta
     6241 ctgatgatgc actttatagg gcaaaattct tcagtaaaaa cagggttgaa ccatatcgct
     6301 cagtacttga agaactaaaa aaagatattg atgacaagca ttttgacctt gttgcttcta
     6361 taaagactct aatcagcgta ataaatgcaa aagatcgcta tacctatgca cataccgaaa
     6421 gagtagtaat attttgtgag ctgattgccc agcaactaaa tctatctgca gaggatagca
     6481 aaatattgaa atacggagca tatatacatg atataggaaa gatccatatc tctgaagata
     6541 ttttaaataa aaaaatgcca ttaaatgata atgagtggaa t
//
LOCUS       DAOSDD010000234         6564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4239_length_6564_cov_5.002305, whole genome shotgun sequence.
ACCESSION   DAOSDD010000234 DAOSDD010000000
VERSION     DAOSDD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_6564_cov_5.002305"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1118
                     /locus_tag="PK733_16085"
     CDS             372..1118
                     /locus_tag="PK733_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ12092.1"
                     /translation="MGKIGLQLYSIKELTQKDFLGTLQQVASIGYDGVEFAGFFDTPA
                     PQLRSVLNAYGLNARGSHTGINLLVKDLEGVIEYNLEIGNPYVICPGLSEEMRNSSDA
                     WKETAELFNNIGSKCKKNGIQFGYHNHAIEFEKFNGEYGYDILAENTDPNLVCLEIDT
                     YWVEYAGLKSIDFMKKYSNRLPLLHIKELKSFSEKESTEIGKGVMNFKEITDLGKKYG
                     TEWYIVEQEHFDIPQLQSIEESLKYLRGII"
     gene            1422..1751
                     /locus_tag="PK733_16090"
     CDS             1422..1751
                     /locus_tag="PK733_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOJ12093.1"
                     /translation="MIYWHIHYFKIDSKLMHGHLHRIAGYTEYVIGTEKFHFHFYSGV
                     STYTDHTHYYSGITGLPVKTENGHYHKIQGLLESNGFHEHKFSGNTYEELTYTHGKET
                     REAFGLR"
     gene            complement(1888..2430)
                     /locus_tag="PK733_16095"
     CDS             complement(1888..2430)
                     /locus_tag="PK733_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ12094.1"
                     /translation="MSEHQEIIIAGFGGQGILSAGRILAYAGMLENKNVSWLPSYGPE
                     MRGGTANCNVIISEEAVGSPILNSATTLIVMNSPSLEKFEKAVIPGGLIIIDSSLVNK
                     KASRTDVEIHEIPATMLASEMGNLTYANIILLGRLIERTRIVSKENFVQALKHILPER
                     KHYLIPEEIKALETGMTYQA"
     gene            complement(2433..3179)
                     /locus_tag="PK733_16100"
     CDS             complement(2433..3179)
                     /locus_tag="PK733_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ12095.1"
                     /translation="MKTVFERPHALKDIQMHYCPGCTHGIIHRLVAEVIDELGIEGKT
                     VGVASVGCSYNNYDYFNCDMAQAAHGRAPAVATGIKRVNPENVVFTYQGDGDLAAIGT
                     AEIIHAAARGEKITTIFINNAIYGMTSGQMAPTTLINQITTTTPYGRKPELHGFPIKV
                     CELLSTLEGAVYIERVSTHDIKNIMNAKKAIKKAFQVQMSNKGFSLVEVLSSCPTNWG
                     MNPVKALSWIKDNMVPVYPLGNFKGKDLEV"
     gene            complement(3372..4427)
                     /locus_tag="PK733_16105"
     CDS             complement(3372..4427)
                     /locus_tag="PK733_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOJ12096.1"
                     /translation="MGEKLLMKGNEVIAEAALRAGCRFYFGYPITPQTEIAHYMAKRM
                     PEVNGTFLQAESEVAAINMVYGAAAAGARVMTSSSSPGISLKQEGISYIAGAELPAVI
                     VNIVRCGPGLGGILPAQCDYFQAVKGGGHGDYKMVVLAPSSIQELYELTVEAFNISDR
                     YRIMTMIMGDGILGQMMETVEFKEKENIIRSDKTWAVTGTAMEREHNTVTSIYINPEV
                     LEKHNQKLQNKYEIIAEKESRVEVYNCDNADIIVTAFGTVARIVKNVIKMAQKEGIKV
                     GLIRPITLWPFPDEAFEKYAQVPKAFLSVELNAGQMIEDVKLAVNGRNKVYFHGRTGG
                     MIPTQQEILNKIKEILA"
     gene            complement(4442..4648)
                     /locus_tag="PK733_16110"
     CDS             complement(4442..4648)
                     /locus_tag="PK733_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ12097.1"
                     /translation="MARVIFDVERCKGCKLCTTVCPKGIVVIKEDVLNMKGFHPAGVN
                     DMDKCIGCAFCATICPDCVIVVEK"
     gene            complement(4742..5410)
                     /locus_tag="PK733_16115"
     CDS             complement(4742..5410)
                     /locus_tag="PK733_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12098.1"
                     /translation="MYYKRINIFTGNFGSGKTEISVNFALKLAGIVEKTAIVDFDIVN
                     PYFRTMDAKKYLEDKGVYVIAPVFANTNVDVPAIPPEVNMLFENKAYTAVFDVGGDDL
                     GAKVISRYKEEIIQCDYAHFFVVNIRRPMTNTIEKIREMICSIEESSGLKITHLVNNT
                     NMLGKTNLNDIMEGNRVVNEIANEMGVKVAFTSGLYNILKEAEEYVDGELFYIEKMIK
                     LPWD"
     gene            complement(5527..6231)
                     /locus_tag="PK733_16120"
     CDS             complement(5527..6231)
                     /locus_tag="PK733_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOJ12099.1"
                     /translation="MDMFSRTEMLIGKENLNKLRKSKVAVFGIGGVGSFVVEGLARAG
                     IGSLILIDSECIDITNINRQIHSNIKTVGRPKVQVMKERILDINPGTEVTIYEKFFCE
                     NTTQELITNDYDYIVDAIDTVSSKIELILAAKKNGIPIISSMGAGNKLDPTKFNVADI
                     YDTNTCPLARVIRKELRKRGIVNLKVVYSTEPPVRAAQQTNYSELKLVPASISFVPSV
                     AGLIIAGEVIKDIMKT"
BASE COUNT         2032 a         1292 c          993 g         2247 t
ORIGIN      
        1 aggatgctgt agctaggcag gggaaactct tctctcaagt aacaggtatt ccttttatgc
       61 tgaagagttc cccgtatctg agtgaagtaa cagcaaaatc agtatttgag ctaaataact
      121 gcaactatta aattattatt tatcaagtta ttttgagagt ttgattttgc ccgtagctga
      181 atttaaaggc tctaagcact gtaacgacag cctctggtaa atattccttt atatttagaa
      241 aatgcacttc cttttgttat ggctgtttaa aatatttgtt aaatatgata atatatttaa
      301 tactactatg ccaatttgga aagcaatgtc attaatccag cactacaaat ttatataagg
      361 agttgattta catgggtaaa attggtttgc aactgtattc tattaaagaa ctgacacaaa
      421 aagatttttt aggcactcta caacaggtag caagtattgg ttacgatggt gtagaatttg
      481 ccggattttt tgataccccc gccccccaat taagaagcgt tcttaatgct tacggcctta
      541 atgcacgtgg aagtcataca ggaataaacc ttcttgtgaa ggatcttgaa ggagtaattg
      601 aatacaattt agaaatcggc aatccatatg taatctgtcc ggggttgtct gaagaaatga
      661 gaaacagttc agatgcctgg aaagaaacag cagaactttt taataatatt ggatcaaaat
      721 gcaagaaaaa tggcatccaa ttcggatacc ataatcacgc tatagaattt gagaaattta
      781 atggtgaata tggatacgat attcttgctg aaaatacaga tcctaacctt gtatgccttg
      841 agatagatac ctactgggtt gaatatgcag gtctgaaatc cattgacttt atgaaaaaat
      901 attctaaccg gcttccactt cttcatataa aggaattgaa gagtttcagt gaaaaggaaa
      961 gtaccgaaat tggtaaaggt gtaatgaatt ttaaggaaat aacagatctg ggtaaaaaat
     1021 atggtacaga atggtatatt gtagaacagg agcattttga tattcctcaa ttacaaagca
     1081 ttgaagaaag cttgaaatac ttaagaggta taatataaat ataacatagt gcatttaaaa
     1141 tttttgaaga aatatattcc agaagctatc attacagtac ttagaacttt cactaatgct
     1201 tcttctaata attgtttcaa aaataaaata tgcactcatc taacatagaa cagaaaataa
     1261 gactttaatt aaaaaatctc attaaacgtg ctgaagttta atgagatttt ttacactttt
     1321 catttatttt ccatcaataa taatttctct tttgcacatt ttaataatga cttgtttttt
     1381 aactgtaaac ctaatattat aataattgag gagtgttctg tatgatatac tggcatattc
     1441 attattttaa aattgattca aaactgatgc acgggcattt gcaccgtatt gccggttata
     1501 ctgaatatgt aattggaaca gaaaaatttc attttcactt ttactcgggt gtatcaacat
     1561 atactgacca tacacattat tactcaggaa ttacaggctt gcctgtaaaa acagaaaatg
     1621 ggcattatca caaaattcaa ggattgttgg aatcaaatgg ctttcatgaa cacaaattta
     1681 gtggtaatac ttacgaggaa ttaacataca cccatggaaa agaaacaagg gaagcttttg
     1741 gattaaggta aggggacaca tatatctaag ttggaggttg gaggctggag gttggttgta
     1801 atttataata attaataaaa aaacggaaac cacttcccac ctcccacctc ccacttttat
     1861 actgaggaat gcctcctgcc attaaactta agcctgataa gtcattccgg tttccaaagc
     1921 ttttatttct tctggtatta aataatgttt tctctcagga agtatatgct ttaacgcctg
     1981 tacaaaattt tccttggaaa caattctggt tctctctatt aatcttccta aaagaataat
     2041 atttgcataa gtcaggttcc ccatctctga tgcaagcatt gtagccggta tctcatgtat
     2101 ttcaacatct gttcttgaag cttttttgtt taccagtgaa ctgtcaataa taattaatcc
     2161 accaggtatt actgcctttt caaatttttc caatgacggg ctattcataa ctattaatgt
     2221 tgttgcacta ttcaatattg gtgaaccaac agcttcctca gaaataatta cattgcagtt
     2281 ggcagtgcct ccacgcattt caggtccgta agaaggcagc catgaaacat ttttattttc
     2341 aagcatacct gcataagcta atattcttcc agcagataat ataccctgtc ctccaaaacc
     2401 agctataatg atttcctgat gttctgacat ccttacacct ccaaatcttt ccctttaaaa
     2461 tttcccaggg gatatacagg taccatgttg tcctttatcc atgataatgc cttgaccgga
     2521 ttcattcccc agtttgtagg acaagatgac aggacctcaa ctaaagaaaa ccccttattg
     2581 gacatttgta cctgaaatgc tttttttatg gcttttttag cattcataat atttttaata
     2641 tcatgagttg aaactctctc tatatatact gcaccttcaa gtgtagataa caattcacaa
     2701 acctttatgg gaaatccgtg taattcaggt ttacggccat atggtgtagt ggttgtaatc
     2761 tgattgatta acgttgttgg ggccatctga cctgatgtca taccataaat ggcattattt
     2821 ataaatattg ttgtgatttt ttctcctctt gcagcagcat gaattatttc tgctgtacct
     2881 atagctgcca gatcaccgtc accctggtaa gtaaaaacca cattttccgg atttactctt
     2941 tttatacctg tagcaacggc aggagctctt ccatgtgcag cctgagccat atcacaatta
     3001 aaataatcat agttattgta gctgcatcct acagatgcga ctccaacagt tttcccttca
     3061 atacctaatt catcgattac ttccgcaacc agcctgtgaa ttattccatg agtacatccg
     3121 ggacagtaat gcatttgtat atcttttaat gcatgcggcc gttcaaaaac agtcttcatt
     3181 ttaagctcca ttcctgctat atgcgaaatg aattaaccct tgcaggaaca aaactttttt
     3241 gtgaaaaagt ttttatgggc taaactcatt ttgtcgcacg tgcaatttat ttttgttgta
     3301 gtcctgtgca aaaacatgca caggtgcatt agtttttcaa aaatttatca atttctttcg
     3361 aactaattcc cctaagctaa tatttcttta attttgttta aaatctcctg ctgagttgga
     3421 atcattccac ccgttcttcc gtggaaatac actttattcc tgccgttaac agcaagttta
     3481 acatcctcaa tcatttgccc tgcattaagt tctacactta aaaaagcttt gggaacctgt
     3541 gcatatttct caaatgcttc atcagggaat ggccagaggg tgataggtcg tattaaacct
     3601 actttaatcc cttctttttg agccattttt ataacattct taactatcct ggcaactgta
     3661 ccaaaagcag taactataat gtcagcattg tcacagttat aaacctcaac tctgctttcc
     3721 ttttcagcaa taatttcata cttgttttga agcttttggt tgtgtttctc aaggacttca
     3781 ggatttatat aaatagatgt tacagtatta tgctctctct ccattgctgt ccctgtaaca
     3841 gcccaggttt tatctgagcg aataatgttc tctttttcct taaactcgac agtttccatc
     3901 atttgcccaa gaattccatc acccatgatc attgtcatta ttctatatct gtctgaaata
     3961 ttaaaagctt caactgttag ttcatacaat tcctgtatac ttgatggtgc cagtactacc
     4021 attttataat ctccatgtcc gccgcctttg acagcctgaa aatagtcaca ctgagccgga
     4081 agaattccac ccaaacctgg cccgcatcta acgatgttta ctataacagc aggaagttca
     4141 gcacctgcta tataggatat accttcctgt ttaaggctaa tccctggact tgatgatgat
     4201 gtcatgactc tggcacctgc agctgcagcg ccatatacca tattaattgc cgccacttca
     4261 ctttcggcct gtaaaaatgt accattaact tcaggcattc ttttggccat ataatgagcg
     4321 atctcagtct gaggtgttat aggataaccg aaatagaatc tgcatcctgc tctcaatgca
     4381 gcttcagcta ttacctcatt tcctttcatt aaaagctttt ctcccattaa ttaagtcccc
     4441 cttatttttc taccactatt acacagtccg gacagatagt tgcacaaaaa gcacagccga
     4501 tgcacttatc catatcatta actccggcag ggtgaaaccc tttcatattg agtacatctt
     4561 cctttataac aacaatcccc ttagggcaaa ctgttgtaca cagcttacat cccttgcaac
     4621 gctctacatc aaaaataact ctagccataa caacctcact tgtaccttaa tattattttt
     4681 gtacattctg taattctttt ttaacattat aaaactcttt ttgttttttt tcaagttccc
     4741 atcaatccca aggaagcttt atcattttct caatataaaa caattcccca tctacatatt
     4801 cctcagcttc tttaaggata ttgtaaagcc cgcttgtaaa tgcaactttt acacccatct
     4861 catttgctat ttcatttact accctgttcc cttccattat atcattcagg ttggtcttgc
     4921 caagcatatt ggtattgttc accagatgag ttatcttaag gcccgaactt tcttctatgc
     4981 tgcaaatcat ttctcttatt ttttctatag tattggtcat aggtcttctt atattaacga
     5041 cgaaaaaatg tgcatagtca cattgtataa tttcttcttt atacctggaa ataacttttg
     5101 ctccgaggtc atcaccacca acatcaaaaa ccgctgtgta agccttattt tcaaataaca
     5161 tatttacctc gggaggtatg gcaggtacat caacattagt attggcaaat acgggagcta
     5221 tgacatatac acccttatct tcaaggtatt ttttagcatc cattgtacga aaatacggat
     5281 taactatatc aaaatctact attgcagttt tttcaacaat tcccgctaat ttcaaggcaa
     5341 aattaactga aatttcagtt ttaccactgc caaaatttcc agtaaatata tttatcctct
     5401 tataatacat taaaaatact ccttctttta ttttattaat tttataataa agtaactcaa
     5461 tatttatata aagtgttatt agtatctgaa tttaatttct acatttaatt atcttttata
     5521 ataccgttat gttttcatta tatctttaat tacctcacct gcaattatca aacctgctac
     5581 ggaaggcaca aaggaaatgc ttgccggtac aagtttaagt tcagaatagt ttgtttgctg
     5641 tgctgcccta actggtggtt cagtagaata tactactttt agatttacta tgcccctttt
     5701 cctgagttcc ttcctaataa ccctggcaag agggcatgta tttgtatcat atatatcagc
     5761 tacattaaat tttgtagggt ccagcttatt tcccgccccc atgctgctta taataggaat
     5821 tccatttttt ttagctgcaa gtataagttc tattttactt gaaaccgtat caatagcatc
     5881 tacaatataa tcataatcat tcgttattaa ttcctgtgtt gtgttttcac aaaaaaactt
     5941 ttcatatatt gtaacttcgg tcccagggtt tatatccaga atcctttctt tcataacctg
     6001 aactttgggt ctgcctacag tttttatatt ggaatgtatc tgtctgttaa tattggttat
     6061 atcaatacat tcactatcta tcagaattaa agacccgata cctgccctgg caagaccttc
     6121 aactacgaaa gatcctacac cgccaattcc aaaaacggca acctttgatt ttctaagctt
     6181 attgaggttt tcttttccaa taagcatttc tgtccttgaa aacatatcca tagttttaat
     6241 accaccttaa gcacgaggat tcttccaaca acccaacttt aatttcataa aaatattatt
     6301 caggggtaca tataatttat ttgaagaaat tattggtaag agtcataagt aaaggctcta
     6361 agtagcgtaa cgacagcccc tggtaaatat ttcttcaaat tcagtaaatg cactcattat
     6421 tcagtaaatt gttgatttaa gttaaataat actataaact atcctattta acaatatttt
     6481 atagaaattt agcatattga cttaaaatct cctgaattct gcacacaaaa aatggaaaaa
     6541 cgcctttgca aagcactaat aata
//
LOCUS       DAOSDD010000235         6561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4243_length_6561_cov_5.500307, whole genome shotgun sequence.
ACCESSION   DAOSDD010000235 DAOSDD010000000
VERSION     DAOSDD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_6561_cov_5.500307"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PK733_16125"
     CDS             complement(<1..1140)
                     /locus_tag="PK733_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12100.1"
                     /translation="MIKAKRIICIIISVLIVVGLFVGCTTGTGKKDNNDVGKTSSKSN
                     ESDNKSTSEDLPTTGFPIVETPITLKIMARYRTGMADFKDMITWQEYEKLTNIKIDWI
                     MIERASAGEKRNLALATGDLPDAFLKCNFSNSDLIKYGAEGTFIPLNDLIDKYAPNYQ
                     KAINDEVNFPDAKRAVTRLDSNIYSFIYLIHNDSVAVKVGPNMYINKKWMDAVGKGMP
                     TTTDEFYDVLKAFKTGDPNGNGQADEIPWYVTGVSGLDPLNGAWGLQNRGTRVGYIDF
                     DENLKKVRFYPADPKYKEMLQFANKLYNEKLIDEEMFTANLADLLAKGSKNMIGAFVA
                     AGNQYVGPEHENDFVGLDYALKGPHGDQNWNANPSALTTGSFVITN"
     gene            complement(1251..2147)
                     /locus_tag="PK733_16130"
     CDS             complement(1251..2147)
                     /locus_tag="PK733_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12101.1"
                     /translation="MGRTYSNHTKGDYIFDIVNTTLLILVLLIVLYPLIFVVSASFSD
                     PLEILDGKVWLLPKGFTLEGYKRVFSNNDIMTGYKNTIIYTLLGTFVNLVMTIAGAYP
                     LSRKDFYGRNIIMLLFTFTMFFSGGLIPTYLIIRKLNMVNKIWVLVLPGAVSMYNLII
                     TKTFFQSTIPLELQEAAVIDGCSNIKILLKIVLPLSIPIIAVMTIFYGVAHWNAFFDA
                     LIYITDRKKFPLQLILREILIQNQMDEMMNSDIENLAQQMMMAEVIKYAVVIVASVPV
                     LMLYPFLQKYFVKGVMIGAIKG"
     gene            complement(2168..3124)
                     /locus_tag="PK733_16135"
     CDS             complement(2168..3124)
                     /locus_tag="PK733_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12102.1"
                     /translation="MRTLISNNTASVRLNRKKNKALKAFSRNYDLYLLILPVILYFAI
                     FKYWPMYGVQIAFKDFIATRGIWGSPWVGIKHFSRFYNSYYFWDLIFNTLGISLYSLA
                     LGFPAPILLALMMNESRSKSLKKIVQTVTYAPHFLSTVVIVSVLVAFLSPRSGLINQI
                     IKLLGRDPIYFMAEPKWFKTLYALSGIWQNAGWGSIIYMAALAGIDTEIYEAAMVDGA
                     SKFKRLIYITLPFLMPTAVTLLILNAGSLMNVGFEKVFLMQNERNLESSDVISTFVYR
                     SGMISAEYSFSAAVGLFNSIINFILLISVNKTSKKLTETSLW"
     gene            complement(3413..5794)
                     /locus_tag="PK733_16140"
     CDS             complement(3413..5794)
                     /locus_tag="PK733_16140"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ12103.1"
                     /translation="MLKPVFANIEKPSKRFYQYLIYYIILLVIIILIISSVVYKYFVS
                     ALQREVETSNLSALNKAAETMDMRIKEINNLALMVSKSSVIHDNLQIGDGFSQGECVK
                     EMAKHIASNMFIYDMLLYYKDKNVNISNKVLTTWGAFSPDDMFNYIYQYKEWGKEDFY
                     EIFNKKAIPYMMPVKKVLVNRGEEELLFTYIFPLPIGSNSPQSAAIFLIKASALNNIL
                     SDVLKNYDGYIYILNEKSEPIYHLTCGNAEGQPLEILNNINSAFSSSTERINIHGKQF
                     SISKAISDYNRWSYIAVMNSSQLFYKVSVVKEIFTMTVIIVFLLGIIIAFVFASKQYK
                     LLRLLANLVKGKTHQFNTPEITYKDEVKIISQCIEYMSDENNKLSMQLKGNIKKVRNE
                     IILSLLKGENKDKIVENEILDMLGIKLDEGSYVISIVFLIDNYKDYRNRNDCLDEKLL
                     KFSIINVLEELSGRVGQGYGVDLIENGKIALILCIKTDLSCEDIIDGIIKETKSIFET
                     HYDISFTIGVGNVYNNVNMIKNSFMEALRASAYRLIYGRGNLIYFSELKAESNNLFKY
                     TTIHEERLRSAIKQAKLEDTFSVIDQIISSIKNCDMIPELVACIYYGVISSLIKIIDE
                     MGVDMIDSSEYGFDDYMSIESFETIDDLKDALEDLCANICSIIQKKWNEKDEITVKAI
                     VNYIKANYSNSSLSLEEISDTFGISPSFCGKNFKTFTGMTIMKYVDDLRFEKAKELLK
                     DTNYNLQYIIDNTGYVDKVNFIRKFRKKEGVTPIQFRNMYRLSIHENIQNDET"
     gene            complement(5978..>6561)
                     /locus_tag="PK733_16145"
     CDS             complement(5978..>6561)
                     /locus_tag="PK733_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12104.1"
                     /translation="ILFSLVILSLIIPVQTIIVPQYIIFSNLKWLNTYLPMIVPTFFG
                     YGLKGGLFIFIFRQFYLGLPTALEDAAKIDGCGFLRTYWNIVLPIAKSAFMVTVVLSI
                     VWHWNDFYEPSIYINLPKLTMLPSRLNAIIAMVNTPPEEILAEVGHAEVESAINNAVL
                     MAGTFLVTLPVLTSFAFLQKQFMQGIERTGIVE"
BASE COUNT         2154 a         1174 c          834 g         2399 t
ORIGIN      
        1 atttgtaatt acaaatgaac cagttgttaa agctgagggg ttggcattcc agttttggtc
       61 accatggggc ccctttaatg cataatctaa tcccacaaaa tcattttcat gttcaggtcc
      121 tacatattga ttccctgctg caacaaacgc accaatcata tttttgctgc ctttagctaa
      181 caaatcagct aggtttgcag taaacatttc ttcatctatt aacttttcgt tatatagctt
      241 gttagcaaat tgaagcattt ctttatattt agggtcagca ggataaaatc ttactttttt
      301 taagttttca tcaaaatcaa tatatccaac tcttgttcct ctgttttgta atccccatgc
      361 tccattaaga ggatctaatc ccgatacacc agtaacatac catggtattt catctgcttg
      421 accattacca ttaggatctc ctgttttaaa tgcttttaac acatcataaa attcatcagt
      481 tgtagtaggc ataccttttc caactgcatc catccatttc ttatttatat acatattagg
      541 tcctactttt acagcaacag aatcattatg tattaaataa ataaatgaat atatgttact
      601 atctaatcta gttactgctc ttttagcgtc tggaaaatta acttcatcat ttatagcctt
      661 ctggtagttt ggcgcatatt tatcaattaa atcatttaaa ggtataaatg tcccttcagc
      721 tccatacttt attaaatcac tgttactaaa gttgcatttc aaaaaggcat caggtaaatc
      781 tccggttgca agggcaagat ttcttttttc accagcactt gcacgttcta tcatgatcca
      841 atcaattttt atgtttgtaa gtttttcata ctcttgccaa gtaatcatgt ctttgaaatc
      901 agccattcct gttctgtatc tagccatgat ctttaatgtg ataggagttt caactattgg
      961 aaaccccgtg gtcggcagat cttccgaagt tgatttgttg tctgattcat tagatttaga
     1021 tgaagttttg cctacatcgt tattatcttt ttttcccgtt cctgtagtac agccaacaaa
     1081 gagaccaact acaattagta ctgagataat gatacaaata attctttttg ctttaatcat
     1141 aatagtacct ccaattcatt ttttatatat tgctataagg aatgtttgtt cctctagtag
     1201 ccaaacataa tttaaagaga tatttggtta atattaatct agaattctgc tcaaccctta
     1261 atagcaccta tcataacacc ttttacaaaa tacttctgaa gaaaagggta aagcattaat
     1321 accggtacgc ttgctacaat tacaactgca tatttaatta cttctgccat catcatttgc
     1381 tgggcgagat tttcaatatc ggaattcatc atttcatcca tttgattttg gataagtatc
     1441 tcccttaata tcaactgtaa cggaaacttt ttacgatctg ttatgtatat tagtgcgtca
     1501 aaaaaagcat tccaatgagc tactccataa aatatagtca taacagcgat tataggtatt
     1561 gaaagaggga gtacgatttt taacaaaatc ttaatattgg agcagccatc gattactgct
     1621 gcttcttgta attctaaagg tatggtactt tgaaaaaatg ttttagtaat tattaaatta
     1681 tacatggata ctgccccagg taacaccaac acccaaatct tattgaccat attgagtttt
     1741 cgtattataa gataagttgg aatgagacct ccgctaaaga acatagtaaa tgtaaataag
     1801 agcataatta tatttctacc ataaaaatct ttccgtgaca gtggatatgc cccggctatt
     1861 gtcataacca aattaacaaa agttcctaag agagtataga ttatggtgtt tttgtatcca
     1921 gtcataatgt cattatttga gaacactctt ttatatcctt ccagagtaaa cccttttgga
     1981 agcagccata ctttcccatc aagtatttcc aaaggatcac taaatgatgc acttactaca
     2041 aaaattaaag gatataaaac aattaataat acaagtataa gtaatgtagt attaactata
     2101 tcaaatatat aatcgccttt agtatgattg ctgtatgttc ttcccatgtc aaagtcccct
     2161 cctctagtta ccataagctt gtttctgtta actttttaga tgttttgttt acagatatca
     2221 gtagtataaa attaataatt gaattaaata atcctactgc tgcagaaaaa ctatattctg
     2281 cgcttatcat accacttctg tatacaaaag tggatattac atcagatgac tcaagattac
     2341 gttcattctg cattaaaaat actttttcaa aacctacatt cataagactc cctgcgttta
     2401 gtataagcaa tgtgactgca gtaggcataa gaaaaggtaa tgtaatatat attaaacgtt
     2461 taaatttact tgctccatct accattgcag cttcatatat ctcagtatca atccctgcta
     2521 atgcagccat atatataata gatccccatc cggcattctg ccatatgccc gaaagcgcat
     2581 aaagagtctt aaaccattta ggttcagcca taaagtaaat aggatctctt ccaagtaatt
     2641 taattatttg gtttattaat ccgcttctag gagataaaaa tgcaaccaat acactaacta
     2701 ttacaacagt agatagaaaa tggggagcat atgttactgt ttgtactatt ttcttaagtg
     2761 atttacttct cgattcattc atcataagtg ccagcaatat aggtgccggg aagcccaaag
     2821 caagtgaata aagacttatt cccagagtat taaatataag gtcccagaaa taatatgagt
     2881 tgtaaaatct gctaaaatgc tttattccaa cccatggact accccaaata cctcttgtag
     2941 caataaaatc tttaaacgct atttgcactc catacattgg ccaatattta aatattgcga
     3001 aatataaaat cactggtaat attaacaggt ataaatcgta gttacgagaa aatgccttta
     3061 atgctttgtt tttttttcta tttagccgca ctgaagcagt attatttgaa attaatgttc
     3121 gcaaaggaat cccccttaaa atgtattctc aataaactat aacaataaac agtaagcttt
     3181 actaaaatta tttgtaagaa taaagctaca tactctaaat aacaattaat ttgataattg
     3241 tcaattacac acaataaaat tttcatcaat tgcattaact gaattatact ttcacttcct
     3301 atttcagtcc atatgcattt cacattttca catgcaataa tttttcgtta tgctaatttt
     3361 cattttttca aggatttctt tttagaattt cttgtattat ttaatgaaat atttaagttt
     3421 catcattttg tatgttttca tgaatactca gcctgtacat attcctaaat tgtatagggg
     3481 ttactccttc tttttttctg aattttctta taaaattaac tttatctaca tatcctgtat
     3541 tatctataat gtattgtaaa ttataatttg tgtcttttaa cagttccttt gctttctcaa
     3601 aacgcaaatc atctacatat ttcatgattg tcattcctgt aaaagtctta aaatttttcc
     3661 cgcagaaaga tggtgatata ccaaaagtat cagaaatttc ttcaagagaa agagagctat
     3721 tactataatt tgcttttata tagttaacta ttgcttttac agttatttca tctttttcat
     3781 tccatttttt ctgaataata ctgcatatgt ttgcacataa atcctctaac gcatccttaa
     3841 gatcatctat agtctcaaag ctctcaatag acatataatc atcaaaacca tactcactac
     3901 tatctatcat atcaactccc atttcatcaa ttatttttat taagctgcta attactccat
     3961 agtatataca tgctactaat tccgggatca tatcacaatt tttaatgctg ctgataatct
     4021 gatctataac tgaaaatgta tcctctaatt ttgcttgttt aattgctgat ctaagtctct
     4081 cctcatgaat agtagtatat ttaaacaaat tattagattc agccttcaac tcactaaaat
     4141 atatcaaatt tcctctacca tagataagtc tgtaagcaga agctcttaga gcttccataa
     4201 atgaattctt aatcatattt acattattgt atacattacc tacaccaata gtaaaactta
     4261 tatcataatg agtttcaaaa atacttttag tttcctttat tattccatct attatatcct
     4321 cacaacttaa atctgtcttt atgcaaagga ttaatgcaat tttaccgttt tctattaaat
     4381 ctacgccata accttgtccc actctgcctg aaagttcttc caaaacatta attatgctga
     4441 atttcagtaa tttttcatct aagcaatcat tccggtttct atagtcttta taattatcaa
     4501 tcaaaaaaac tatagaaata acgtaacttc cttcatcaag ttttatgcca agcatatcaa
     4561 gtatttcatt ttcaacaatt ttatctttat tctcaccttt taacaaagat aatattattt
     4621 catttcttac ttttttaata ttacctttaa gctgcatact taacttgtta ttctcgtcac
     4681 tcatgtattc tatgcattgt gaaataattt tgacctcatc tttgtatgta atttcgggcg
     4741 tgttaaattg atgtgttttt cctttaacca ggtttgctaa taatcttaat aatttatatt
     4801 gcttggacgc aaaaacaaat gctattatta ttcctaaaag aaaaactata ataacagtca
     4861 tagtgaatat ttctttaaca acacttactt tgtaaaacaa ttgactagaa ttcattacag
     4921 caatatatga ccacctattg taatcagaaa tcgcttttga tatagaaaat tgtttcccat
     4981 ggatatttat tctttctgtg ctagaactaa atgcactatt tatattgttt agaatctcta
     5041 gtggctgtcc ttcagcattt ccacaggtta aatgataaat aggctcactt ttttcattta
     5101 atatatatat atatccgtca tagtttttca ggacatcact taaaatatta tttaaagcag
     5161 aagcttttat tagaaagatt gctgcacttt gaggactatt actgccaatt ggcaaaggaa
     5221 atatatatgt aaatagaagt tcttcttcac ccctgtttac taatactttt ttaacaggca
     5281 tcatgtatgg aatagctttt ttattaaata tttcataaaa gtcttcttta ccccactcct
     5341 tgtattggta aatatagttg aacatatcat ctggtgagaa agcaccccag gttgtcaaga
     5401 ccttattgct aatattaaca ttcttatcct tataatatag taacatatca tatataaaca
     5461 tgttacttgc tatatgtttt gccatttctt ttacacattc accttgacta aacccatctc
     5521 caatttgtaa attatcatga ataacactac ttttcgatac cattaaagca aggttattaa
     5581 tttctttaat acgcatatcc atcgtctcgg cagctttgtt taaggctgaa agattggatg
     5641 tttctacttc tctttgcaaa gccgatacaa aatacttata tacaacactg cttattatta
     5701 gtattatgat cacaagcagg ataatataat aaattaaata ttgataaaac ctctttgaag
     5761 gtttctctat atttgcaaaa acaggtttta acaaagctat ttctcctttt tatttatcat
     5821 atgctgccta ataaagctgt tccattttga aacttctatt ctatcatgct ttataatttt
     5881 tagtattttt tattaattta ttttactata gatttatggc aataactaca tacaaaatat
     5941 atttttagta cattttttaa cttatacatt atttatctta ctctacaatt cctgttcttt
     6001 caattccttg cataaattgt ttttgtaaga atgcaaaaga agttaaaacc ggcaatgtaa
     6061 ctagaaatgt tcctgccatt aaaacagcat tgtttattgc actttctact tctgcatgac
     6121 ctacttcagc cagaatctcc tcaggtggtg tattaaccat agctattatt gcattgagcc
     6181 tgctaggaag catagttaac tttggcaaat taatgtaaat tgagggttca tagaaatcat
     6241 tccagtgcca tactatggaa agaactacag taaccataaa agcagatttt gctattggca
     6301 aaacaatatt ccaataagtt ctgagaaagc cgcagccatc aatttttgca gcatcttcca
     6361 gtgccgtagg tagtcctaga taaaattgtc taaatataaa tataaacaat ccacctttta
     6421 atccatagcc aaaaaatgtt ggtactatca taggtaagta agtattaagc catttcaaat
     6481 ttgaaaatat tatatattga ggaacgatta ttgtctgtac aggaataata aggctgagaa
     6541 taaccagtga aaagagaatc t
//
LOCUS       DAOSDD010000236         6533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4268_length_6533_cov_5.261500, whole genome shotgun sequence.
ACCESSION   DAOSDD010000236 DAOSDD010000000
VERSION     DAOSDD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4268_length_6533_cov_5.261500"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1242
                     /locus_tag="PK733_16150"
     CDS             259..1242
                     /locus_tag="PK733_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOJ12105.1"
                     /translation="MNDASPVIRDFLNYMGTIKGKSKNTIKEYYYDLRTFFRFMKQYK
                     NIVNPNTEFDSINIDDIDIDFIKTITLSDLYEFMSFLSRERDNASNSRARKVASIKSF
                     FNYLANKAKLLEYNPASELESPKVMKRLPKYLNVDESKKLLEVIDGKHRERDYAIITL
                     FLNCGLRLSELVNININNIKEDTLTVIGKGNKERTIYLNNACISAINSYMQVRPVDSV
                     KDKNALFLSERKQRISNKTVQHIVKKYILMSGLDPERYSAHKLRHTAATLMYKYGKVD
                     IRALQQILGHESVATTEIYTHVDNTQLRNAVDSNPLAEIKMDEKLDISTDE"
     gene            1461..2393
                     /locus_tag="PK733_16155"
     CDS             1461..2393
                     /locus_tag="PK733_16155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ12106.1"
                     /translation="MSLRKFYFAFTSILLIFLFSTGIVILNYLKAYSAKEALSPDGNV
                     TDNLLKPFFIDKDPFNALILVGDKSEANTDTMMVVNYNPSTSEMNIMSIPRDTKVTLD
                     NGKNVKINSLYVNRGGSTLLMKHLSNMLGIDIKYYVYLNLETFRKVIDLLGGVWIDVP
                     IDLDYVDPVQNLHIHVKAGKQLMGGNKAEEYLRFRHPNGGYSKEMLQYYDGSDLKRIE
                     AQQKFIKELVKQKANILYIHKLNSIIDTVFENLDTNISLSEVLKMVKTVKDFGPDKVA
                     LHTLPGYSMDASPWYYLYDKDQTKELVKENFYIK"
     gene            2650..3195
                     /locus_tag="PK733_16160"
     CDS             2650..3195
                     /locus_tag="PK733_16160"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOJ12107.1"
                     /translation="MMLENACFPLPSEIILPLAGNMAAIGIVDFFWVSLVANAGALIG
                     SLIAYSIGYYGGRPFILKYGKYVLISESKLFRAEKSFNNIGISAIFIGRLFPLLRTFI
                     SIPAGLAKMDIKKFVIFSLIGIIPWNLSLIFLGYKFGKNYATKLQPIFNKLKIPAIIT
                     TVLFIFVYILYKQKSSRQKLI"
     gene            complement(3298..4392)
                     /gene="mnmA"
                     /locus_tag="PK733_16165"
     CDS             complement(3298..4392)
                     /gene="mnmA"
                     /locus_tag="PK733_16165"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOJ12108.1"
                     /translation="MAGKKVMVGMSGGVDSSVAAALLLEKGYDVIGVTMQIWQDTSEK
                     YSENYEGCCSLSAVDDAIRVANKLGIPHYVMNFKDIFKENVIDYFINDYLKGYTPNPC
                     IACNKYIKFGALMNKALSMGVDYVSTGHYARIEYNSNDGRYLLKKAVTKEKDQSYVLY
                     NLTQGKLGRVLLPIGEYVKEQVRNLAKEFDLPVAAKPDSQEICFIEDNNYSAFINRNT
                     QLDIKSGYFTDTKGNILGKHKGIVHYTVGQRKGLGITFGKPMFVVEIDAENNTVVLGT
                     ADEVFSKSLTAVDLNFISIDNLSSEMKVDAKIRYSAKEAPATIYPLEEGKVKVVFDSP
                     QRAVTPGQSIVFYQGETVVGGGIISKSDRL"
     gene            complement(4493..4954)
                     /gene="nifU"
                     /locus_tag="PK733_16170"
     CDS             complement(4493..4954)
                     /gene="nifU"
                     /locus_tag="PK733_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOJ12109.1"
                     /translation="MYSEKVMDHFMNPRNVGEIKDADGVGQVGNPKCGDIMKMYLKIE
                     NDIIVDAKFKTFGCGAAVATSSMATELVIGKSIDEALKITNKAVAESLDGLPPQKMHC
                     SNLAEEAIKAAIDDYKIKNGLIEDVNINQACSRRCDDVEAHLHDFNLDDKE"
     gene            complement(4999..6177)
                     /gene="nifS"
                     /locus_tag="PK733_16175"
     CDS             complement(4999..6177)
                     /gene="nifS"
                     /locus_tag="PK733_16175"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOJ12110.1"
                     /translation="MPEKYIYFDHAATTSVKPEVLEAMMPFFSEKFGNASSVYGYGRE
                     SKKYIEDSRDKVASALGSQSKEIFFTGSGTEADNWAIKGVAYGNRTKGNHIITTSIEH
                     HAVLHSCNYLEKEGFEVTYLPVDKNGLIDPEQVREAIRPETILVSIMFANNEIGTIQP
                     IKEIGSITKEKGILFHTDAVQAVGNIKINVEDLNVDLLSLSAHKFYGPKGIGALYIRR
                     GTKIVPFMHGGAQERGRRASTENVPGIVGLGKAIEIANSNIDEYNRKLIGLRDRTINE
                     VLNKIPYVRLNGHSEKRLPGNVNFSFEFIEGESLLLMLDMKGIAASSGSACTSGSLDP
                     SHVLLAIGLPHEIAHGSLRITFGDENTQEDVDYFMSILPEIINKLRDMSPLYEAVKKQ
                     "
     gene            complement(6299..>6533)
                     /locus_tag="PK733_16180"
     CDS             complement(6299..>6533)
                     /locus_tag="PK733_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12111.1"
                     /translation="KLSLCHYISQIVDTIEEDLYGGAGIMKKRIIAWVMLSGFVLLLV
                     NILIIGYQRILSVLVYLVIAFYYILFMNKKSRV"
BASE COUNT         2119 a         1160 c          967 g         2287 t
ORIGIN      
        1 tttttatata cgattataca aaataaaaat tttgtataat cgggtgtagg taaataagca
       61 aaaatgaacc cttaacattt aatcaatact gccaataaaa ctttccataa ttcttataat
      121 gtactataat ataatcatta gtataaacat acataagcat atttaagcac atttaggtat
      181 atttatttat aatttgctat gttgttctta ttacttttgt aaattgtttt tattattttt
      241 gtaaagggag aatttttaat gaatgatgca tcacctgtta ttcgtgactt tttaaattat
      301 atgggtacga ttaaaggtaa atcaaaaaat acaattaaag aatattacta tgatcttcga
      361 acctttttcc ggtttatgaa gcaatacaag aatatcgtca accccaatac tgagtttgat
      421 agtattaata ttgatgacat tgatatagat tttataaaaa cgattactct cagtgattta
      481 tatgagttta tgtctttcct gagtcgtgaa cgtgataatg cttctaattc tcgtgctcga
      541 aaggtagcca gtattaaatc ttttttcaat tacttagcta acaaagcaaa gctgttagag
      601 tataatcctg catccgaact ggaatctcct aaagttatga agcgtttacc gaaatacctg
      661 aacgttgatg aaagtaaaaa acttcttgag gttattgatg gaaagcatcg cgaaagagat
      721 tatgctataa ttaccctgtt tttaaattgt ggccttcgac tatcagaatt agtgaatata
      781 aacataaata atattaaaga agataccctt actgttatag gtaaaggcaa taaagaacga
      841 actatttact taaataatgc ctgcataagc gctataaatt catatatgca agtaagacct
      901 gttgatagcg taaaagataa aaacgcactg tttctaagtg aaagaaaaca gcgtataagt
      961 aacaaaacag tacagcacat tgtaaaaaaa tatattttaa tgtccggact tgaccctgaa
     1021 aggtattcag ctcacaagtt aagacatact gcagcaacgc tgatgtataa atacggcaaa
     1081 gtggatattc gcgccctgca gcagatactg ggtcacgaaa gtgttgcaac aacggaaatt
     1141 tatacccatg tagataatac tcaactgaga aacgctgtag atagcaatcc tttagcagaa
     1201 attaaaatgg atgaaaaatt agatattagt acggacgagt aggagcgcag gaatgcatac
     1261 ttgactaaat tgagtatccc ttcccccttc cctacctgct ttgtaatata aaaacaacaa
     1321 aataataaag aaaactttac tatgaaaact ttactataca agggatttca tttataatat
     1381 taaggattgg tataatattt tggtataata ttgtacaatg ttaaggttta ataatataga
     1441 ttttaaggag tctgtttttt atgagtttga gaaagtttta ttttgctttt acatcaatat
     1501 tgcttatatt cttgttttct acaggtatag taatacttaa ttatttgaaa gcatattctg
     1561 caaaggaagc attatcgcct gatgggaatg taacagataa tttattaaaa ccttttttta
     1621 ttgataaaga tccatttaat gctttaattc tcgtaggaga taaatcggaa gctaatactg
     1681 atactatgat ggtagtaaat tacaaccctt ctacaagtga aatgaatatt atgtccattc
     1741 cacgtgatac aaaagttaca cttgataatg gcaagaatgt aaaaatcaac tctttgtatg
     1801 taaacagggg cggaagcact ttgcttatga agcatttaag caatatgctt ggaattgaca
     1861 ttaaatatta tgtatattta aatcttgaaa ccttcagaaa agtaattgat ttactgggag
     1921 gtgtatggat agatgtacct attgatctgg attatgttga tcctgttcag aatctccata
     1981 ttcatgttaa agccggaaag cagctaatgg gtggtaataa ggctgaagag tacctgcgat
     2041 tcaggcatcc taatggcggg tattcaaaag aaatgctcca gtactatgat ggctctgatt
     2101 tgaagagaat tgaagctcag caaaaattta taaaggaatt ggtaaagcaa aaagcaaata
     2161 tattatatat acacaagctt aatagcatta ttgatactgt attcgaaaat ttggatacca
     2221 atatatcttt aagtgaagtc ttaaaaatgg ttaaaactgt taaagacttt ggtcctgata
     2281 aggttgctct tcatactctt ccaggttatt ctatggacgc ttccccatgg tattacttgt
     2341 atgataaaga tcaaacaaaa gaattggtaa aagagaactt ttatattaaa tgacgtatga
     2401 cgtatattga tgtatattag ttcagtttag aattatataa gaagtataga gttatataaa
     2461 atgaagtaaa tatattttct aatgcatttt tagatacctg tctgaataat aattattaat
     2521 ccagtaatta atttaagcgg agataaaata attaattata tgacaggtgt tttttatttt
     2581 gcagaaaaat ttatagatta tattattaag gttgttaatt ctacaggtta tccaggggta
     2641 tttattggaa tgatgcttga aaatgcatgt ttcccattac caagcgaaat aattttacct
     2701 cttgccggta atatggcagc cattggaata gttgattttt tttgggtaag tctggtagca
     2761 aatgcaggag ctctaattgg ctcgttaatt gcctactcta taggatatta tggtggaaga
     2821 ccatttatat tgaaatatgg aaaatatgtt ttaatatctg aatctaaatt atttcgggca
     2881 gaaaaatcat tcaacaatat tggtatatcc gctatattta taggcagatt gttcccctta
     2941 ttaagaacct ttatatccat ccctgcaggt ttggcaaaaa tggatataaa gaagtttgtt
     3001 atattctcac ttataggaat tattccatgg aacttgagtt taatatttct aggttataaa
     3061 tttggtaaaa attatgccac taaattacag ccaatattta ataagttaaa gatacctgca
     3121 ataataacta ctgtactttt catttttgtc tatatattgt ataaacaaaa atcatcaaga
     3181 caaaaactta tatagaacag ttttaaaaca tttaaaacct gcttcctaca acctgtagcc
     3241 atctaccatc cactatctac catctactat ccctcaattc ttacttacca cttttaccta
     3301 taatctatca cttttactta taattccccc tcctactact gtctccccct gataaaatac
     3361 tatagattga cccggtgtaa cagcccgctg tggagaatca aaaaccacct ttacttttcc
     3421 ttcctctaat ggatatattg ttgcgggcgc ttcctttgcc gagtacctga tttttgcatc
     3481 tactttcatt tcactactaa gattgtcaat ggatatgaag tttaggtcaa cagctgtcag
     3541 ggattttgaa aacacctcat cggcagtgcc taacacgact gtattatttt ctgcatcaat
     3601 ttctactaca aacataggct tgccaaatgt tattcccaga ccttttctct gccctacagt
     3661 ataatgtaca atgcccttat gtttacctaa aatattaccc tttgtatctg taaaataccc
     3721 ggattttata tccaattgtg tgtttctgtt aataaatgca ctataattat tatcttcgat
     3781 aaaacatatt tcttgactat caggttttgc agctacaggt agatcaaatt cctttgcaag
     3841 atttctaacc tgctctttta catattcacc aataggtaat agcactcttc ccagctttcc
     3901 ctgtgtaaga ttatataaca cgtaggattg atctttttct ttagttacag cctttttcag
     3961 aagatacctg ccatcattgc tgttatattc aatccttgca taatgacctg tagaaacata
     4021 gtcaactccc atagacaatg ctttattcat taaagcgccg aatttaatat acttattaca
     4081 tgctatacat gggtttggag tatagccctt taaataatca tttataaaat aatcaataac
     4141 gttttcttta aatatatcct taaaattcat aacatagtgt ggtatgccaa gtttatttgc
     4201 aacccttatg gcatcatcaa cggcagataa ggaacagcag ccctcataat tttcagagta
     4261 tttttcactt gtatcctgcc agatttgcat agttacgcct attacatcgt aacctttttc
     4321 taaaagcagg gcggctgcaa ctgaactgtc tacgccgcca ctcataccta ccattacttt
     4381 ttttcccgcc attataattt attatacctc tttcttaact tatcttgttt gcttaatact
     4441 acatttgatt gtattaattt gataaacttg tccgatttct tttcattacc ctttactctt
     4501 tatcgtccaa attaaaatca tgcaaatgtg cctcaacatc atcacacctt ctgctgcagg
     4561 cctgatttat gtttacatcc tcaatcaggc cattttttat tttataatca tcaatagccg
     4621 ccttaatagc ttcttctgcc agatttgaac agtgcatttt ttgtggcgga agtccgtcaa
     4681 gggattctgc aacagcttta ttggttatct taagagcttc atcaatactt ttaccaataa
     4741 ccagttccgt agccatgctg cttgttgcta cagccgcacc gcagccaaat gttttgaatt
     4801 tagcatctac tataatatca ttctctattt tgagatacat tttcataata tccccgcact
     4861 tgggattccc gacctggcct acaccatccg catctttaat ttctccgaca tttcttggat
     4921 tcataaaatg atccataact ttctcactgt acatataaat tccctctttc aatgttatac
     4981 gattatacga ttttaagtct actgcttttt cactgcctca tataaagggg acatatctct
     5041 taacttatta ataatttcag gcagtatgct cataaaataa tctacatcct cctgtgtatt
     5101 ttcgtctcca aatgttattc tcagtgaacc atgagctatt tcatgaggca gacctattgc
     5161 aagcagcaca tgggaaggat ccaaagatcc tgatgtacat gctgagccac ttgaagcagc
     5221 aatacctttc atatcaagca taagcaaaag agattccccc tcaataaact caaaggaaaa
     5281 attaacatta cccggcaatc tcttttcaga gtgtccattt aggcgtacat agggtatttt
     5341 gtttaaaacc tcattaatag tacgatctct taagcctata agcttcctat tgtattcatc
     5401 aatattgctg ttggcaattt cgatggcttt acctagacct actataccag gaacgttttc
     5461 cgtactggcc cttcttcctc tctcctgagc tcctccatgc ataaatggta caattttggt
     5521 tcctctcctt atatataatg cgccaatacc tttggggcca taaaatttat gagccgaaag
     5581 agataaaagg tcaacattaa gatcttctac atttatttta atatttccaa ctgcttgtac
     5641 tgcatcagtg tgaaataaaa tgcccttttc ttttgtaata ctaccaatct ctttaattgg
     5701 ttggattgta cctatttcat tgttagcaaa cattattgaa accaatattg tttcgggcct
     5761 gatagcttct cttacctgtt caggatcaat cagaccattt ttatctacag gtaaatatgt
     5821 tacttcaaag ccttcttttt caagatagtt gcaagaatgg agaactgcat gatgttctat
     5881 tgatgttgta ataatgtgat tgcctttagt acggttacca tatgcaactc ctttaattgc
     5941 ccaattatca gcttcagtac cagagcccgt aaagaatatt tctttgctct gagaacctaa
     6001 agctgaagca actttatctc ttgaatcttc aatatatttt ttgctttctc ttccataacc
     6061 atatactgag gaagcatttc caaatttttc actaaaaaaa ggcatcatcg cctctaatac
     6121 ttccggtttt actgatgttg tagctgcatg atcaaaatat atatattttt ccggcatatt
     6181 gcattctcct taaaaaaatt gttactagtg ttaatatttt aaatcatttt atatattata
     6241 tgctttcata ttatcttacc cttttttaat acagactaac actatattta ttttaatttt
     6301 aaaccctgct ttttttattc ataaacaata tgtagtaaaa tgctattact aaatatacta
     6361 atacgctaag aattctctga taaccaataa taagaatatt tactagcagc aaaacaaatc
     6421 ctgataacat aacccatgca ataatacgtt ttttcattat accagcccct ccatataaat
     6481 cttcttctat agtatcaact atttggctta tgtaatgaca tagacttaac ttt
//
LOCUS       DAOSDD010000237         6524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4274_length_6524_cov_5.630855, whole genome shotgun sequence.
ACCESSION   DAOSDD010000237 DAOSDD010000000
VERSION     DAOSDD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_6524_cov_5.630855"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1212)
                     /locus_tag="PK733_16185"
     CDS             complement(85..1212)
                     /locus_tag="PK733_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12112.1"
                     /translation="MNHRERALAVLNYEKYDRLPLVHFGFWNELLDKWHEEGHLTKEE
                     AKKWSDGNEIDRVISTKLGFDFNWSRTYGGNTDLFPGFESKVLEVFPDGTRKEINGHG
                     VIILKKDGVVSIPKEVDHMFKDRSSWEEHYLPKLQYTEKRINFSALEAIKADEENGIA
                     RDTPLGIYCGSLYGRVRDWLGIEGISYLYADDEELYDEIINTVGEICYKITKKILSSG
                     VKFDFGHFWEDICFKNGPLINPSVFKEKIGPHYKKITKLLNEHGINIVSLDCDGCIDA
                     LIPTWIENGVNTMFPIEVGTWNASIAPWREKYGKDLRGVGGMDKRVFAYDYDAIDQEV
                     ERLKPLVELGGYIPCPDHRLPLNAKWENVQYYCDRMKKTFG"
     gene            1553..2350
                     /locus_tag="PK733_16190"
     CDS             1553..2350
                     /locus_tag="PK733_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12113.1"
                     /translation="MPLYIHGMGHFHSGKSYFTEREGLDNYLLMFTISGCGYLKYKGE
                     EYLIEPNQAVIFYCYEHQLYRTASEDLWQFKWIHLNGAAVSEYYEVLNDEGLNIVTIR
                     NINEINSRFDNIYDIVDTVDKKNIYSDIKICTELVNLISALIIEKYYHINDKKNYQNK
                     REIEKVLNYIHSNYSQRINIDEITKKVFISKYHFLRLFKLHTGLSLYEYIINFRINMS
                     KKLLKETESSVSDIAAAVGFGDTNNFIRYFKRLVGTTPGNYRRYWIN"
     gene            2455..3153
                     /locus_tag="PK733_16195"
     CDS             2455..3153
                     /locus_tag="PK733_16195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ12114.1"
                     /translation="MFPLRDNVPSYKKPYVTCLIIAVNTIIFLIQFIMPEKLNELFVY
                     VYGFVPAIFSKAVIENTLTGQNFYPFFTSMFLHGNWLHLISNMWTLWLFGDNIEDRLG
                     HFRFIIFYLLCGLSAMVCHFIFYPGSPVPAIGASGAIAGVMGAYFILFPYARIQTLFI
                     PIFIIPVFLRIPAVIYLGIWFLTQLYNGAINSVLGGRGSGVAWWAHIGGFIGGIVLLK
                     LMYKRKYRRPVYYE"
     gene            3249..3944
                     /locus_tag="PK733_16200"
     CDS             3249..3944
                     /locus_tag="PK733_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOJ12115.1"
                     /translation="MRDFISGAKKGIPIALGYIPVSFTFGLIAVNGGIPVWIAILISM
                     TNLTSAGQFAGTDLIIAGSSFVEIALTTFIINIRYMLMSLSLSQKVDQKMPVSKRSIM
                     AFGITDEIFSIASMEEGEITFSYMMGLFSCPYWGWALGTALGALTCSVLPEALQNSMG
                     IALYAMFIALVVPAAKKSKAAFVVAAIAISINSILTWMPGLNSISDGWSIIIATLISC
                     TAGALLFPRKSEV"
     gene            4119..4430
                     /locus_tag="PK733_16205"
     CDS             4119..4430
                     /locus_tag="PK733_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOJ12116.1"
                     /translation="MQGILAAVLLMAVVTYIPRAFPMVFLRRKLKSEFLRSFLYYVPY
                     AVLGAMIFPKIVYSTGNIYTALAGMIAALIMAYFNKGLMKVAISAILVVYISGLLFKL
                     A"
     gene            4559..4954
                     /locus_tag="PK733_16210"
     CDS             4559..4954
                     /locus_tag="PK733_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOJ12117.1"
                     /translation="MRINSTDAVVLNTDSIAGSNFRPVDTGITAVEGVRKTRPAARNS
                     SSMTELEKRELPVSEKAVIDAVERANKVVSIANRRFEYSIHEETKQIMVKVIDVETEE
                     IVREIPPEKILDLVATIWEMVGLIVDERR"
     gene            4959..>6524
                     /gene="fliD"
                     /locus_tag="PK733_16215"
     CDS             4959..>6524
                     /gene="fliD"
                     /locus_tag="PK733_16215"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ12118.1"
                     /translation="MDTGINLLTSKFRIGGLATGFDTDKMVSDLMKIERMPLDKLCQK
                     KQLAEWKRDGYRDITNLLRSLKDEFFDILKPSNYLLSQSTYKKLSATSSDSSMLTATA
                     SAQAMTGIYNIEVKKLATADQIESSDLVTVPVKSNAPLTLEGVKNASGKNIVVVLDGI
                     SKEITLGDFTGAETLENALSQLADDLESKINSAFGTGKVNISIDGDYLTLTTTGGSSK
                     LTLTNGYSNDGLSLLNITPGSSNRLNMYQTLESLSKSGSTDLKFNDEGNLEFEINSKK
                     FTFSKTTTLANMLSTINSDEDAKVTITYDEITDKFVIKSRALGAGNNITISQTGGNFF
                     GSADPSDSFSKISIEPDRIGNGKDAVIVINNTTVTRSSNNFTLNGITYNLHKADEGKT
                     HTITVSTDVDGVYDSIKGFVDKYNEIIDIINGKLAEKYEREYQPLTNEQKEALSEKET
                     ERWEKRAKTGLLKNDSILESIVINMRRALFDKIEGVDINVTSIGITTGRYEEKGKLKI
                     DEQKLKTAIRENPD"
BASE COUNT         2162 a         1071 c         1245 g         2046 t
ORIGIN      
        1 tccgttgctg aagtgaatat tatacatgga aatcgtttct tgccatattt tccctaaaaa
       61 gtaaagtatg cacccatggt tttctcatcc gaaggtcttc ttcattctat cacaataata
      121 ttgtacattc tcccacttgg catttaaagg caatctgtgg tcggggcagg gaatatagcc
      181 acctagttct accagcggct tcaaccgctc tacttcctga tctatggcat cataatcata
      241 ggcaaaaact cgcttatcca ttccgcctac accacgcaag tcctttccat atttttcgcg
      301 ccatggagct atacttgcat tccaggtacc tacttcaatt gggaacatag tatttactcc
      361 attttcaatc catgttggta taagggcatc tatacagcca tcgcagtcaa gggaaactat
      421 atttattcca tgttcattga gcaatttggt tattttttta taatgaggac ctatcttttc
      481 tttgaaaaca gatggattta tcagcggccc gtttttaaag cagatatctt cccaaaaatg
      541 gccaaagtca aactttactc ctgatgaaag tatttttttt gtaattttgt agcaaatctc
      601 accgacagta ttaataattt catcatacag ctcctcatca tctgcataga ggtagcttat
      661 gccttcaatc ccaagccagt ctcttactct gccgtataaa ctaccgcaat aaattccaag
      721 gggggtatcc cgtgctatac cattttcttc atcggctttt attgcttcaa gtgcagaaaa
      781 gttaattctt ttttcagtgt actgaagctt tggaagataa tgctcctccc agcttgacct
      841 gtctttaaac atatgatcaa cttccttggg aattgaaaca acaccgtctt ttttgagaat
      901 aataacacca tgaccattta tttcttttct ggtaccatcc gggaaaactt caagtacttt
      961 tgattcaaat cctggaaaca gatcggtatt tcccccataa gttctggacc agttaaaatc
     1021 aaaacccagc tttgtggata ttactctgtc gatttcgttt ccatcactcc actttttagc
     1081 ttcctctttt gtaagatgac cttcttcatg ccatttatca agaagctcat tccagaaacc
     1141 gaaatgtact aaagggagtc tgtcatactt ttcataattt aatacagcta atgctctttc
     1201 acgatgattc atttattgct cctcctatat gttattttat tgaggcagca gatgaagaca
     1261 cttttgctga atttaactga aaacaagctg cgcacaaaat ctaataaaaa taatgacaaa
     1321 agtctgatta aaatactaat attgaaaagt tgctataata atattacaat atttactata
     1381 ataatataat tataatctac tataaaagac aattgtcatt ataaattgct ataaaatatg
     1441 tatatattgc tatgaaagga taggtgaaga cagaattaag acagaatatt gtttagatat
     1501 tactggtgaa tataaatcta taagtcatac accaagcatc agggcacagc ggttgccgtt
     1561 atatattcac ggtatgggac atttccattc tgggaaatcc tatttcactg aaagagaagg
     1621 gcttgataat tacctgttaa tgtttacaat atcaggttgt ggatatctta aatataaagg
     1681 ggaggaatat cttatcgagc ctaatcaagc agttatattc tattgctacg aacatcagct
     1741 ttaccggact gcttcagaag acctatggca gtttaagtgg atccacttaa acggagcagc
     1801 tgtaagtgaa tactatgagg tattaaatga tgaaggctta aatattgtta caattagaaa
     1861 tatcaacgaa ataaacagca gatttgataa tatatatgat atagttgata ctgttgataa
     1921 aaagaatata tactctgaca taaaaatatg tacagaactt gtaaatttaa tatctgcatt
     1981 aattatagaa aagtattatc atataaatga taaaaaaaat taccagaaca aaagggaaat
     2041 cgaaaaggtg ttgaattata tacattccaa ttattctcaa aggattaata ttgatgaaat
     2101 tactaaaaag gtgtttatca gcaagtacca ttttttaaga ctatttaaat tacatacagg
     2161 tttgagcctt tatgaataca taattaactt tagaattaat atgtctaaaa aactattaaa
     2221 ggaaacggag tcaagtgtaa gtgatatcgc cgctgctgtg ggctttgggg atacaaacaa
     2281 tttcataaga tatttcaaaa ggctcgtagg tacaactccg ggtaattaca gaaggtactg
     2341 gataaattaa atgtacccgt aaactataaa atattgacaa ttacagcatg cagtgcgatt
     2401 atattgatgt gataaaccaa tagctcaaat ttttaagaag attggagatt ttatatgttc
     2461 cctttacgag ataatgtacc gagttataaa aaaccctatg ttacttgttt aataatagcg
     2521 gtaaatacca taatcttttt gattcagttt attatgcctg agaagcttaa tgaactattt
     2581 gtatatgtat acggttttgt tcccgctatt ttttcaaaag ctgtcataga aaatacatta
     2641 acggggcaga atttttatcc tttttttaca agcatgtttc tccacggtaa ctggcttcat
     2701 cttataagca atatgtggac attatggctt tttggggata atatagaaga caggctgggg
     2761 cattttcggt ttataatatt ttatctttta tgcgggttat ctgccatggt atgccatttt
     2821 attttttacc cgggttctcc tgtgccggca ataggagcgt cgggtgcaat agcaggagtc
     2881 atgggggcgt actttattct ttttccttat gccaggatac aaacactgtt tatccccata
     2941 tttattattc ctgtttttct tagaattcca gcagttatat acctaggcat atggtttctt
     3001 acacagttgt ataacggagc tataaattcg gttcttggag gcagaggcag cggtgtggct
     3061 tggtgggccc atattggggg atttataggc ggtatagtac tgcttaagct aatgtacaag
     3121 aggaaatacc gcaggccggt atattatgaa taaaatagaa taaatcagcc ggtgtattat
     3181 gaataaatta gaattagtct gtacactatt aattgttgat tttcaaatag aacggagtgt
     3241 tagaaataat gcgagacttt atatcagggg ccaaaaaagg aatacctatt gccctcgggt
     3301 atattcctgt ttcttttacc tttggcctta tagcagttaa tggggggata cctgtatgga
     3361 ttgccatatt gatttccatg acaaacctga cttctgcggg acaatttgct ggaactgatt
     3421 tgattatagc aggatcttct tttgtggaga ttgccctgac tacttttatt ataaatatac
     3481 gatatatgct gatgtctctg tccctttcac agaaagttga tcaaaaaatg ccggtttcaa
     3541 aaagaagtat tatggccttt ggaataaccg atgagatttt tagtattgct tctatggaag
     3601 aaggagagat aactttttca tatatgatgg ggctattttc atgtccatat tggggttggg
     3661 cattgggaac agctcttggg gctttaacct gttccgtttt acctgaagcc ctccaaaatt
     3721 ctatgggtat agctctttat gcaatgttta tcgctcttgt agtacctgca gcaaagaaat
     3781 caaaagctgc atttgttgta gcagccatag caatttctat caattccata ttgacatgga
     3841 tgcctggcct taacagcata tcagatggtt ggagtattat aattgctacc cttatctcat
     3901 gtactgctgg tgcgttgcta tttccaagaa agtctgaagt ctgaggagtg attaaaattt
     3961 ttgaaaatat aataaccaaa aatataataa ccagaaatat aatttttgag aatatatcag
     4021 ccggtagtat tgcggtacat aatatactag aaaataacat tataaatggt agtaatatat
     4081 atatgtgtgg taataatata catacaactg gcaccaatat acagggaata cttgcagcag
     4141 ttttgttgat ggctgttgtt acatacatac caagggcttt tcccatggta tttttacgta
     4201 gaaaactgaa gtcggaattt ctccgctcat ttctttatta tgtgccatat gcagtattag
     4261 gtgcaatgat attccctaaa atagtttatt caacaggaaa tatatatact gcgttggcag
     4321 gtatgatagc agcacttatt atggcatact ttaataaagg tttgatgaaa gttgcaatta
     4381 gtgcaatatt agttgtatat ataagcggat tgcttttcaa attggcataa tagtattaat
     4441 ttatgaagat atatgatata tatcatatta ttaatgccag atttttttct aatgattgta
     4501 aaccctctct gatatttacc gatatatata ttaggtaaat actcacggag ggatttttat
     4561 gaggataaac agcacggatg cggttgtatt aaatactgat agtattgcag gaagtaattt
     4621 cagacctgtt gatacgggaa ttactgcagt ggaaggtgtg agaaagacta gacctgcagc
     4681 aagaaactct tcttcaatga cagaacttga gaaaagagaa ctgcctgtta gtgaaaaagc
     4741 tgtcattgat gctgtggaga gagccaataa ggtagtatcc atagctaata ggcggtttga
     4801 atattccatt catgaagaaa caaaacaaat aatggtaaag gttattgacg tagagactga
     4861 agaaattgta cgggagattc cacccgaaaa aattctggat ctggtagcta ccatatggga
     4921 aatggttggg cttattgttg atgaaagaag gtgagaaaat ggataccgga attaatttat
     4981 taacttcaaa attccgcata ggcggtctgg ctacaggatt tgatactgat aaaatggtca
     5041 gtgatctgat gaaaatagag agaatgcccc ttgacaagct ttgccagaaa aaacagcttg
     5101 ctgaatggaa gcgggatgga taccgggata ttaccaacct tttgagaagc ttaaaggatg
     5161 aatttttcga tattttaaag ccatccaact atctgctttc tcaatctaca tataaaaaac
     5221 taagcgccac aagttcggac agttcaatgt taacagccac tgccagtgca caggcaatga
     5281 ccggaatata caatatagaa gtgaaaaagc tcgcaactgc tgatcaaatt gagagttccg
     5341 accttgtcac agtacctgtt aaaagcaatg ccccactgac actggaggga gttaaaaatg
     5401 caagcggaaa gaatattgtt gttgtactcg atggtatttc aaaagaaata acactaggag
     5461 attttactgg ggcagagacg ttagaaaatg cactctctca acttgctgat gatttagaaa
     5521 gtaaaattaa ctcggccttt ggtactggta aagtaaatat aagcattgat ggagactatc
     5581 taacccttac aacaactggc ggctcaagta aattaactct gacaaatggc tactccaatg
     5641 atgggcttag cttattaaat attactcctg gttcaagtaa caggctcaat atgtatcaga
     5701 ctttggaatc actctctaaa agtggaagca cagaccttaa atttaatgat gagggaaatc
     5761 ttgagtttga aattaactcc aaaaagttta ctttttctaa aacaactaca cttgctaata
     5821 tgctaagtac tataaatagt gacgaggacg caaaagttac aattacttat gatgagatta
     5881 cagataagtt tgtaataaaa tccagggctc ttggagcagg aaacaacatt acaatatctc
     5941 aaaccggagg gaatttcttt gggtcggcag acccatcgga ctcattttcc aaaatttcta
     6001 tagaacctga ccgtatagga aatggcaagg atgcggtcat agtaataaac aacacaacag
     6061 ttacgagaag cagtaataat tttactttaa atggaatcac atataatctg cataaagctg
     6121 atgagggaaa aactcataca attactgtaa gtactgatgt tgatggtgta tatgatagta
     6181 ttaagggctt cgtagataag tataatgaaa taattgatat cataaatggc aagctggcag
     6241 aaaaatatga gagggagtat cagcctctga caaatgaaca aaaggaggct ttaagcgaaa
     6301 aggaaactga aaggtgggag aaaagggcaa agacaggact gctgaaaaat gacagcatac
     6361 tggaaagtat tgttattaac atgagaagag ctctatttga taaaattgaa ggtgttgata
     6421 ttaatgttac cagtataggt ataacaacag gcagatatga agaaaagggc aagcttaaaa
     6481 ttgacgagca aaaacttaaa actgcaatta gagaaaaccc ggat
//
LOCUS       DAOSDD010000238         6491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4304_length_6491_cov_4.811684, whole genome shotgun sequence.
ACCESSION   DAOSDD010000238 DAOSDD010000000
VERSION     DAOSDD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_6491_cov_4.811684"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK733_16220"
     CDS             complement(<1..218)
                     /locus_tag="PK733_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ12119.1"
                     /translation="MKAYFSVFKMRLINGMQYRVAALAGVATQFFWGFMFIMIYEAFY
                     QSAVQTPPIELKQLVSYMWLQQAFLVFIV"
     gene            complement(236..1213)
                     /locus_tag="PK733_16225"
     CDS             complement(236..1213)
                     /locus_tag="PK733_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ12120.1"
                     /translation="MITVEGLSKTFMVPKRTAGFKAAVKSLFYREYTEIQALDDVSFK
                     IDEGEIVGYIGPNGAGKSTTIKVMSGILVPDKGICRIMNHTPWKERVLHVKNIGVVFG
                     QRSQLWWDVPVVDSFELLKDIYNIPYDEYKKNVDMLVETLDLSNFINSPVRQLSLGQR
                     MRCEIAASLLHSPKILFLDEPTIGLDAVSKIAVRQFIKSINKEKKVTVILTTHDMNDI
                     EALAERILLIGKGKILYDGSLNKLRSRFGTNKTITVEFMENSTLPEIQGTKILSWLPQ
                     RASLYIDTQEISVSEVISALAEQLELSDVTVENPPIEEIIVQLYKEYEI"
     gene            complement(1277..2092)
                     /locus_tag="PK733_16230"
     CDS             complement(1277..2092)
                     /locus_tag="PK733_16230"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOJ12121.1"
                     /translation="MIKKILHHLDVGCLYLKLTLQSQMEYPMFLFSWIISNPMQFLVG
                     AWTLKALIDQFQTINGWNFGQVAFLYGLGSITHGLSIIFFIQTWGIGWAVTEGGFGRY
                     LLRPLNVFFQFAFSYFNMIGITDLIPATIVFAYGCIQISFQWTLINTFKILLVIIGGT
                     LIRGAVYIAVGSIAFWTKRSGSLITISLTLMEKNVMYPVSIYPTIIQFIFTFCVPLAF
                     ICFYPVSELLGMNTYFTFPGSLCVWTLGIGILVYFMSMMVFKKGLKHYDSSGS"
     gene            complement(2098..2889)
                     /locus_tag="PK733_16235"
     CDS             complement(2098..2889)
                     /locus_tag="PK733_16235"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOJ12122.1"
                     /translation="MKAYFYMVKIRMQLSLTYRFEFITSIFVQIVILIANSFLWRVLY
                     ANKTTVSGVNMKQMLTYSIMSVLISCIFYYDVENGLRSRIRRGDIAIDILKPINIFAM
                     YFFNDVGNALNAVIQRILPLLLIAALFIQLPLPASPTHLLLFMVSTAFSFLIMWLMGV
                     LFGMINFWVINLGPLGNIKEMIIRLLSGSIIPVWFFPVWIQKVFSFLPFIYTYQQPLG
                     IYVGKFTMGEALFGIEIQLMWVLILYGGYRIMKNRALKNVLIQGG"
     gene            complement(2965..3942)
                     /locus_tag="PK733_16240"
     CDS             complement(2965..3942)
                     /locus_tag="PK733_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ12123.1"
                     /translation="MALIEVKNLSKEYKVVKKKSGLRGAFAGLLHRETTIVNAVNDIS
                     FSIDKGEIVGYIGPNGAGKSTTIKMMSGILAPTKGSILINGISPQKDRQQVVKNLGVV
                     FGQRSQLYWDLRLGESFELLKRIYQIPDETYKSNLAVMNEILKINEIIDTPVRQLSLG
                     QRMRGDLAAAILHSPPILFLDEPTIGLDVEVKYAIRKFIRDINLKFQTTIILTTHDLD
                     DIQQLCDRIIIINHGRIVEDGSLHELIKRISPYRQLVLEFYDEVPEIKHEKAKVIKQE
                     ISRVWLQFDNTKITAADLISDLSVNYTFKDLSLEEPNIDEIIREVYSKT"
     gene            4220..>6491
                     /locus_tag="PK733_16245"
     CDS             4220..>6491
                     /locus_tag="PK733_16245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOJ12124.1"
                     /translation="MDKAATNAIDNIDNIDKVVHRISVRNLVEFILRSGDINLGGYIS
                     SQMMHEGTMIHKKIQKEASDDYKAEVRLSLTREYNDYILEVSGIADGVVTTKEGVTID
                     EIKTVSVPLDYIDGDYSPMHWAQAKCYAYIHAAQSGIDKIYIRLTYFNTETKKMKYII
                     KEFSYCELESFFNELIRMYSIWSGFIVNHKKDRDDSISRLLFPYKSYRKGQRDLAVSV
                     YYAIRDKSKLFARAPTGIGKTISVLFPSLKALGNGMGEKIFYLTARTITRQAAEDALI
                     TMRDKGLNIKHVTITSKDKICPNETRSCNPIDCIYARGHFDRINDAILDILKNESIIT
                     REAIISYSQKHMVCPFEYTLDISNWVDCIVCDYNYAFDPRAYLRRYFEDGGDYIMLVD
                     EAHNLVDRAREMFSVSLTKSDFMKLKRDIKESAPDVYRSLNMINKFFIELRKKFEKRY
                     ETLTIDLPTDIYQLINNFIEECESYLFQHGNSKINDALLELFFGCLSFNAVYRLYDHR
                     YITYITRTDSDVTIKLFCIDPSHLLSEACKKGRTSIFFSATLMPLKYYFDMLGGDESD
                     RVAVLPSPFDKNNMCLMVIGNLSTRYKDREESLMAIINYIKSTADQKLGNYLVFFPSF
                     EYMNTATVLYKESWPDDQIIIQNRSMTEKGREDFLLSFKEQPDKTLIGFAVLGGIFSE
                     GIDLIGEKLSGAIIIGVGLPQICDERDIIKDYFDETNRMGYDYSYRFPGMNRVLQAAG
                     RVIRAEQDRGFIMLIDD"
BASE COUNT         2062 a         1267 c         1220 g         1942 t
ORIGIN      
        1 acgatgaaca ccaagaaagc ctgctgtagc cacatatagc tgactaattg ttttaattct
       61 ataggtgggg tttgtacggc actttggtaa aaggcttcat aaatcatgat aaacatgaaa
      121 ccccagaaga attgtgttgc tacacccgct aatgccgcaa ccctatattg cattccattt
      181 ataagacgca ttttaaatac tgaaaaatat gctttcatgc ctctcccccc ttatttcata
      241 tctcatactc tttatataac tgcacgataa tttcttctat gggcgggttt tccacagtga
      301 catccgacaa ctcaagctgc tccgctagag cggatataac ttctgaaaca cttatctcct
      361 gtgtatcgat atacaagctt gctctttgag gcagccagga taatatttta gttccttgta
      421 tttcgggtag tgtgctgttt tccatgaact caaccgtaat agttttattc gttccaaatc
      481 tactacgcaa tttgttcaag ctgccatcat ataatatttt acccttgcca ataagcagga
      541 ttcgctcagc cagagcctca atatcattca tatcatgagt agtcagtata actgtaacct
      601 ttttttcttt gttgatactt tttataaact gccgaactgc aatctttgaa acagcatcca
      661 gaccaatagt tggttcatcc agaaaaagta ttttagggct atgcagtaat gatgcagcaa
      721 tttcgcagcg cattctctga ccaagactta actgacggac agggctgttt atgaaattgc
      781 ttaaatcaag agtttcaaca agcatatcaa cattcttttt gtattcatca taaggtatat
      841 tatatatgtc tttcagcagt tcaaaggaat ccactaccgg aacatcccac cagagctgtg
      901 atctttgtcc aaaaaccaca ccaatattct taacatgcaa aacccgctcc ttccatggag
      961 tatgattcat tattctgcat atacctttat ccggcactaa aataccgctc atcactttta
     1021 tggttgttga ttttccggca ccattgggtc cgatatatcc aacaatttcg ccttcatcaa
     1081 ttttgaaaga tacatcatca agtgcctgaa tttctgtgta ttctcgataa aacagtgatt
     1141 ttaccgcagc tttaaagcct gctgtacgct ttggaaccat aaatgtttta ctcaaacctt
     1201 caacagtaat catatttccc ccattgcaat taatatgcca tctgtaaagt acctgtaaca
     1261 atatcgtttt cttttatcat gaccctgaac tgtcataatg cttcaaccct tttttgaaca
     1321 ccatcatgga cataaaatat actaagatac cgattcccaa tgtccacacg caaaggcttc
     1381 ccggaaaggt aaaataagta ttcatgccca aaagctcact tacgggataa aaacagatga
     1441 atgccagcgg aacacaaaat gtaaagataa attgaataat cgtcggatag atgctgacag
     1501 gatacataac atttttttcc attaatgtaa gactgatggt tattaatgag ccgcttcgtt
     1561 ttgtccagaa ggcgattgag cccacagcta tgtaaacggc accacggatc agggttcctc
     1621 cgatgattac cagtaaaatt ttaaaagtat tgatcaacgt ccattgaaag gatatttgaa
     1681 tgcacccata tgcaaagaca attgtcgcag ggataaggtc tgttatgcca atcatgttaa
     1741 aataactgaa ggcaaactgg aaaaacacat tcagcgggcg cagcagataa cggccaaagc
     1801 ccccttctgt aacagcccag ccgatacccc acgtttgaat gaagaaaatg atggaaagac
     1861 catgggttat agaacccaga ccatataaaa atgccacctg gccgaaattc cagccattga
     1921 tggtctgaaa ttggtcaatg agagctttca aggtccatgc accgactaaa aattgcatgg
     1981 ggtttgagat aatccaactg aacaaaaaca tcggatattc catctggctt tgtaaggtaa
     2041 gcttgaggta taagcaccct acatccaaat ggtgcagtat tttttttatc atacacatca
     2101 acctccctgt atcaggacat ttttcagagc cctgtttttc atgattctgt aaccgccgta
     2161 aagaatcagt acccacataa gctgaatttc tattccgaac agtgcttcac ccattgtgaa
     2221 ttttcccaca taaatgccca gaggctgttg ataggtatag ataaagggca aaaaggagaa
     2281 cactttctga atccacacag ggaaaaacca gacagggata atgctgcctg acagcaggcg
     2341 gatgatcatt tccttaatgt tgccgagagg gcctaaatta atgacccaaa aattaatcat
     2401 gccaaatagc actcccatca accacataat aagaaagctg aaagcagtgc ttaccataaa
     2461 cagcaaaaga tgggttggag aggctggcaa tgggagctga ataaatagag ctgcaataag
     2521 cagcaatgga agaattcgtt gtatcacggc attcagcgca tttcctacat cattaaaaaa
     2581 atacattgca aatatattaa tcggtttcaa aatgtctatt gcaatatccc cccgtctaat
     2641 tcgggaacgt agaccgtttt ccacatcata atagaaaatg cagcttatca gcacgctcat
     2701 aatggaatag gtaagcattt gcttcatatt gacaccggaa actgtagttt tgtttgcata
     2761 aagcacgcgc cataaaaacg agtttgcgat taagatgacg atttgaacga agatagaagt
     2821 aataaattca aacctgtacg tcaaagatag ctgcatccta attttaacca tataaaaata
     2881 ggctttcatc ggtttcctcc ttgtatcaaa tgattttatg tactgtttat tattatttat
     2941 gtactgttat tttttgtaat tgatttaggt tttactgtaa acctcacgga tgatttcgtc
     3001 gatattcggt tcttccagac tcagatcctt gaaagtataa tttactgaaa gatccgatat
     3061 cagatctgct gcagtgattt tggtattgtc aaactgaagc cagactctgg aaatctcttg
     3121 tttgatcact ttcgcttttt catgcttgat ttcaggcact tcatcataga attccagaac
     3181 cagctgtcta taaggagaaa tgcgtttaat cagttcatgt aaggagccat cttctacaat
     3241 ccgaccatgg tttatgatga tgattctgtc acagagctgc tggatatcat ccagatcgtg
     3301 cgtggtcaga atgattgtcg tttgaaattt taggttgata tcccggatga atttgcggat
     3361 tgcatattta acctctacat ccagtccgat ggtcggctca tctaaaaaca gaattggcgg
     3421 agaatggaga attgcggcag caaggtcccc cctcattctt tgacccagag aaagctggcg
     3481 cacaggggta tctatgattt catttatttt caatatttca ttcattacgg ccagattact
     3541 tttgtaagtt tcatcaggaa tctgatagat gcgcttgagc agttcaaatg attcccccag
     3601 gcgtagatcc caatagagct gtgagcgctg cccgaagaca acaccgagat tttttacaac
     3661 ctgctggcgg tctttttggg gcgaaattcc gttgataaga atacttccct tggtgggagc
     3721 caggattccg ctcatcatct taatggtggt acttttgcct gcgccattag ggccaatata
     3781 tccgacgatt tctcctttgt cgatcgaaaa gctgatatca tttactgcgt tcacgattgt
     3841 agtctcacgg tggagcaaac ctgcaaaagc accacgcaga ccactttttt tcttaactac
     3901 cttgtattcc ttagataggt ttttaacttc gattaaagcc attttttatc atcttctttc
     3961 ttgtatcaat agcctaaatg gtcataatgt aaaagcttat cgttccgaat cagcctcttt
     4021 gcataattac atttttatat ggaaaattat ttcacaatac actcatattt gtcaagaaga
     4081 tttcgtgctt aatcccaggt atctccacaa attgtagtgt taagaattat ttgactttca
     4141 aagcaaacat aggatataat ataaggcatt cgaacatggg ttcgtaatat cttgagaaat
     4201 tgtataaagg gtacagaaca tggataaagc tgctactaat gctattgata atattgataa
     4261 tattgataaa gttgtacata gaatatcagt cagaaatctg gtcgaattta ttcttcgatc
     4321 aggcgatata aatttgggtg gttatatatc aagccagatg atgcatgaag gaaccatgat
     4381 ccacaagaaa atccagaagg aagctagcga tgattataaa gctgaagtaa ggctttccct
     4441 aacaagggaa tataacgatt atatattgga agtaagtggt attgctgatg gagtggtcac
     4501 tactaaagaa ggggtaacaa tcgatgaaat taaaacagtc tccgttccac tcgattatat
     4561 agatggagat tacagcccca tgcactgggc tcaggctaaa tgctacgcat atatccatgc
     4621 tgctcagtca ggaatagaca aaatatatat caggttgacg tatttcaaca ctgagaccaa
     4681 aaaaatgaaa tatataataa aggaattctc ctactgtgag ctggaatcat tctttaatga
     4741 attaattaga atgtattcaa tttggtctgg gtttatagtt aaccataaaa aagacaggga
     4801 tgattcaatt tcaaggctgc tatttcccta taaatcatac agaaaaggcc aaagagattt
     4861 agctgtctca gtatattatg caataaggga taaaagtaaa ttatttgcaa gagccccaac
     4921 aggtatcgga aagactatat ctgtcctatt tccatcactc aaagcactgg gaaatggtat
     4981 gggtgagaaa atcttctacc ttacagccag gacaataacc aggcaggcag ctgaagatgc
     5041 tcttattact atgagagata agggtcttaa tataaagcat gtaaccatta catcgaaaga
     5101 taagatatgt cctaatgaga cccgttcatg caatcctata gattgcatat atgcaagagg
     5161 tcattttgac agaatcaacg atgcaatcct ggatatcctg aaaaatgaat caattataac
     5221 aagggaagct attatttcat attcacagaa acacatggta tgtccctttg aatatacact
     5281 agacatatcc aactgggttg actgcattgt ctgcgactat aattatgcct ttgaccccag
     5341 ggcatatctc aggcgctatt ttgaagacgg cggagattat ataatgctgg tcgatgaggc
     5401 tcacaacttg gttgacagag ccagagagat gttctctgtc agcttgacta aaagcgattt
     5461 tatgaaactt aaacgtgata ttaaagaatc tgctccagat gtgtatcgtt cattaaatat
     5521 gataaacaag ttctttatag aattgaggaa gaaatttgag aaaagatatg aaacattaac
     5581 cattgacttg cccactgata tatatcagct tattaacaat tttatagaag aatgtgagtc
     5641 ttatctattt caacacggaa attcaaaaat aaatgatgct cttctcgaac tcttctttgg
     5701 ctgtctgtct tttaatgctg tttacaggct ttatgaccat cgatacataa catatataac
     5761 acgtacagac agtgatgtta caataaaact attctgtatt gatccatcac atttgctcag
     5821 tgaagcatgt aaaaaaggca gaacctcaat ctttttttca gccacactta tgcctctaaa
     5881 atattatttt gatatgctgg gtggcgatga atcagacaga gtagccgttc ttccttcccc
     5941 atttgacaaa aacaacatgt gtcttatggt aatcgggaat ctttctacaa ggtataagga
     6001 tcgtgaagaa agcctgatgg caattataaa ctatattaaa agcactgcag accaaaaatt
     6061 gggtaattac cttgttttct ttccctcgtt tgaatatatg aatactgcaa ctgtgctata
     6121 caaagagagc tggccggatg atcaaattat tatacagaac agaagtatga ctgagaaggg
     6181 aagagaagat tttctattgt cttttaaaga acagcctgat aaaactttga tcggatttgc
     6241 agtgttaggt ggtatatttt cggaaggtat cgatctcatt ggtgagaagt tatcgggtgc
     6301 tattatcata ggagtcggtc taccccagat atgtgacgaa agagatatta ttaaagacta
     6361 tttcgatgaa accaacagaa tgggctatga ttattcttat aggtttcccg gaatgaaccg
     6421 ggtccttcag gcggcaggaa gagttatacg agctgaacag gatagaggat ttataatgct
     6481 tatcgacgac c
//
LOCUS       DAOSDD010000239         6478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4318_length_6478_cov_6.847268, whole genome shotgun sequence.
ACCESSION   DAOSDD010000239 DAOSDD010000000
VERSION     DAOSDD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4318_length_6478_cov_6.847268"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="PK733_16250"
     CDS             <1..2022
                     /locus_tag="PK733_16250"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOJ12125.1"
                     /translation="YQTMKEYDRFEKGAANGNTSTRVLQRQLEDRDEKGNPHEYKIIV
                     TTIQKLDIFIRKNKQHDIYKKHVVLIFDECHRSQFGEMHQAITKSFKNYHIFGFTGTP
                     IFAANASSGGNPLLRTTEQAFGEKLHTYTIVDAINDGNVLPFRIDFINTIKMPDYVND
                     KKVYSIDREKALADPQRISEIVSYVLEHFDQKTKRNSYYTFSAKWEEADKHNPKKMIE
                     KRETRRVAGFNSIFAAASIPMAIRYYNEFKKQIAEKNRNLTIATIFSFSANEEEPDGL
                     LPEEDFNMENLDQSSRDFLEAAIRDYNSTFSTNYDTSSDKFQNYYKDLSLRVKNREID
                     ILIVVNMFLTGFDATTLNTLWVDKNLRQHGLIQAFSRTNRILNSVKTYGNIVCFRDLK
                     EETDKAIALFGNKDAGGIVLLKTYEEYYNGYDDKGEYKPGYAELITTLTTQYPLGQPI
                     LGEEAEKDFIRLYGAILRLRNILTSFDDFEGNEILSERDFQDYQSIYIDLYQEYRKGA
                     DGDKETINDDIVFEIELVKQIEVNIDYILMLVAKYQQSNCKDKTILTTIDKAINSSIE
                     LRSKKELIERFIEQVNVSTKVDEDWRKFLHERKEADITAIIEEERLKPEETRRFIDNA
                     FRDGILKTTGTAIDKIMPPVSRFGGGRAAKKQGIIEKLMLFFEKYLGLI"
     gene            complement(2146..2505)
                     /locus_tag="PK733_16255"
     CDS             complement(2146..2505)
                     /locus_tag="PK733_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12126.1"
                     /translation="MAVINTSKGDKIFEIVNIFCVTLMMVLVLYPLVYIVSCSISDPN
                     LVGRGEVWLYPKGLTLDGYKRVFRDENIMVGYKNTLIYTICGTMLNLFMSLTAGYALS
                     KKTLPGRGWIKKRSLRD"
     gene            complement(2547..3464)
                     /locus_tag="PK733_16260"
     CDS             complement(2547..3464)
                     /locus_tag="PK733_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12127.1"
                     /translation="MKSRIKDLIRSINKHRTLYIFLLPAVLYVAIFSYLPMYGLQIAF
                     KNYSGALGIWGSKWVGLKHFKDFIGGYYFWPIIRNTLTISIYSTVVSFPFPIILALML
                     NEVNNYKYKKFLQTVLYAPHFISMVVMVGIIILMLSPSIGIINHFISFLGFEKQYFMI
                     QPDAFKHIYVWSGIWQDMGWGAVIYIAALSSVNQELHEAATIDGASRIKRIWHINLPA
                     IKPTIIILLILNIGSIVSVGFEKTFLLQNDLNLAKSEVISTYVYRRGLLKGSLSFSTA
                     VGLFNNVINLILLVLTNYIARKLNETSLF"
     gene            3786..5981
                     /locus_tag="PK733_16265"
     CDS             3786..5981
                     /locus_tag="PK733_16265"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ12128.1"
                     /translation="MTLKNFLIKHNYLIKLSISLVIAIYLPGIFIGLGVIKSSYNEIV
                     NKSENKYYDMTTSFSAYFIEQINFLKKHALTIIYDNENAPVEKKIMKDIIDSHPYYYL
                     TTTKILSDYRIGLPNAIDIGICFKGSDYIITSTFKYKISDFMKISSTNNTYNTKELEE
                     FFNYDIDRIKLASNFNCRNVTEANFLVGVPVVFNKQHEALIIYIMRYDSVNTTFFGKE
                     EIENVSLLIFNADGQLAFASDPKSNDFYHNISGNDAFRKYLLNSDTKIESFTIGYNFT
                     AMKVYNSNIRKIFVSIIPENLMEENLNQYLSIVYKTTTLAMMAFALLLGISVYINYKP
                     IFNLVKPIRKKNPINLSSEIRTISTAMDKMEKNVAEQKILLMDYLLGNILYGIPISEE
                     IMDRLDFNRSSDLYCVFVIMDIKMNSRERDRITNYLYSKCNIHAYVTDILNKNHIILI
                     CTLKNNDVSIILSELKKYLNEIYEFVYKIGVGHIVNNIGDIRKSYLNALRIVELTMIN
                     VNLLGSEISFTENYPTEEINLFLQYIENSEKENAFGILDNIFKYINSIEISLLRQYIA
                     CDLLISYIKCLKKIKYPFDKKDEFMLFNNNSTAELFEAMRISVSTVCDVILHNNEVSY
                     SNLQKEIIEYVNQNFTSFDLCRTQVADIFNISIYTLSRIFKENAGLGFKEYVLSKRLE
                     LSKALLMNTNKSITQITIEVGFSDPGYFSRIFKMTYGTTPSQFRSGNSL"
     gene            complement(6165..>6478)
                     /locus_tag="PK733_16270"
     CDS             complement(6165..>6478)
                     /locus_tag="PK733_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ12129.1"
                     /translation="KGDLHHIVRKNAHFFYYFELGILVVNALRRSGVCGYRSVLLALL
                     ICILYAVSDEVHQIFIPGRSGEIRDVIIDSTGSSVGILGFLGVSWVAGSMNRKVERIR
                     Q"
BASE COUNT         2300 a         1103 c         1064 g         2011 t
ORIGIN      
        1 tatcagacta tgaaggaata tgaccgtttt gaaaaggggg cagctaacgg taatacgtcc
       61 acaagagttc ttcaaagaca attggaagac agggacgaaa aaggaaatcc tcatgaatac
      121 aaaatcattg taaccactat tcagaagctg gatatattta tacgtaaaaa caaacagcat
      181 gatatttata agaaacacgt ggtactgatt tttgatgagt gccaccgttc ccaatttggt
      241 gaaatgcatc aagctatcac gaagagcttt aagaactacc atatcttcgg ctttacggga
      301 actccgattt ttgctgctaa tgccagttca ggaggtaacc cactgcttcg tacaaccgag
      361 caggcctttg gagaaaagct acatacctat accattgtgg atgccatcaa tgatgggaat
      421 gttctgcctt ttagaataga ttttatcaat accattaaga tgcctgatta cgttaatgat
      481 aaaaaagtct atagcataga tagagaaaaa gccttagcag atccacagcg aatcagtgaa
      541 attgtttctt acgttcttga acactttgat cagaagacga agcgcaatag ttattacaca
      601 ttctctgcga aatgggaaga agcagataag cacaacccta aaaagatgat agaaaagcgt
      661 gaaacaaggc gagttgccgg tttcaactcc atatttgctg ctgcatccat cccaatggca
      721 atcagatact ataatgagtt taagaagcag atagcggaaa agaaccgtaa ccttaccatt
      781 gcaactattt tcagttttag tgcaaatgag gaggagccag atggactgct tcctgaagag
      841 gattttaata tggaaaacct tgaccagagc tccagagatt ttcttgaggc ggctatccga
      901 gattacaatt ccacatttag cacaaattac gatacttcct cggataagtt ccagaattat
      961 tataaagacc tctctcttcg tgtaaagaat cgggagattg atatattgat tgttgtcaat
     1021 atgttcctga caggctttga tgcaacaacc ctaaataccc tatgggtaga caaaaacctg
     1081 agacagcatg gactgattca ggctttttca agaactaacc gcatcttaaa cagcgttaag
     1141 acctatggta atatcgtttg ctttagagat ttgaaagaag aaacagataa agctattgca
     1201 ctattcggca ataaggatgc tggcggtatt gtactgctaa aaacatacga agaatactat
     1261 aacggctatg atgacaaagg tgagtataag ccgggctatg ctgaattgat tacaactctt
     1321 acaacacagt atccccttgg acaacctatt ctcggagaag aagctgaaaa agactttatt
     1381 cggctatatg gagcaatact caggctcaga aatattctta cttctttcga tgactttgaa
     1441 ggaaatgaga ttttatctga aagggatttt caggactatc agagcattta tattgacctg
     1501 tatcaggaat acagaaaagg tgctgatggt gacaaagaaa ctatcaatga tgacatagtt
     1561 ttcgagattg aactggtcaa acagatagag gtaaatattg actacatcct tatgcttgta
     1621 gccaaatatc aacaatccaa ctgcaaggat aaaaccattc ttacgaccat tgataaggct
     1681 atcaattcga gtattgaact tcgaagtaag aaagaactta tcgagcgttt tattgagcaa
     1741 gtcaatgtat cgaccaaagt ggatgaggat tggcgtaaat tcctccatga gcgtaaggaa
     1801 gcggatatta cagcaattat agaagaagaa agattaaagc ctgaagaaac ccgccgtttc
     1861 atcgacaatg ctttccgaga tgggatactt aaaacgaccg gtacagctat tgataaaatc
     1921 atgccacctg tatctcgctt tggcggaggc agagccgcga aaaagcaagg aattatcgaa
     1981 aaactaatgt tattctttga gaagtattta ggattgatat agattaatat caatcgctaa
     2041 ttcaaaaata atatctttat aaaaaaccag tcacttcaag cattcatgcc tgattgtgac
     2101 tggtctcttt ttgctccgct tgaagctgtt catttaagtt tttcttcaat ccctaagtga
     2161 acgcttcttt atccatcccc ttccaggcag tgttttttta gaaagtgcat accctgccgt
     2221 aaggctcatg aataaattta acattgtccc acatattgta tatattaacg tgtttttata
     2281 acctaccatt atattctcat ctctaaatac tcttttgtat ccatcaaggg ttaagccttt
     2341 gggatatagc caaacctcgc cgcgtccaac taaatttgga tcgcttattg aacaggatac
     2401 tatatatacc agcggatata ataccaatac catcataagg gtgacacaga atatgttaac
     2461 tatctcaaaa attttatcgc ctttgcttgt attaattaca gccatatctt tctcctttct
     2521 taagccagga taaacccgga atattttcag aataaacttg tttcatttaa ttttcgtgca
     2581 atataattgg tcagtacgag gaggattaag tttataacat tattaaataa tccgactgct
     2641 gtagaaaagc ttaaactgcc tttcaaaagt cctctccggt aaacatacgt ggatattact
     2701 tcagacttcg ccaaattaag atcattttgg agtaaaaatg ttttttcaaa tccaacgctt
     2761 acaatgctcc cgatattcaa aataagcagt attattattg ttggttttat tgcaggtaaa
     2821 tttatatgcc aaattctctt tatacggctt gcgccatcta tcgtagcagc ttcatgtagc
     2881 tcttgattta ccgaagatag tgcagcaata tatataacag ccccccaacc catatcttgc
     2941 caaatgcctg accatacata tatatgttta aatgcatctg gctgaatcat aaaatattgc
     3001 ttctcaaacc ccaaaaaaga aataaagtgg ttaatgatgc ctattgaagg tgacagcata
     3061 aggatgataa ttccaaccat aacaaccatt gagataaaat gaggtgcata taatactgtt
     3121 tgcaagaact ttttatattt ataattattt acctcattta gcattaatgc gagtataata
     3181 ggaaaaggga agctaacaac agttgaatat atggaaatgg ttagcgtatt ccggattatt
     3241 ggccaaaaat aatagccccc gataaaatcc ttaaaatgct ttaatccaac ccatttactc
     3301 ccccaaatac ctagagcccc gctataattt ttaaaagcga tttgcagtcc atacataggt
     3361 aaatatgaaa atatcgctac atacaaaact gcagggagta aaaaaatata taaagtccta
     3421 tgtttgttaa tactccttat tagatcctta attctacttt tcatggttaa ttcatcctcg
     3481 aatctataaa taatttattt aaaaaagata atataataat acaagatggc taaagccctt
     3541 tcaattggat atttgtaaat ctatattgga taaatgtatc ataattagct ttgtaatgtg
     3601 tttgctagaa aactacaata aacttgcaca aatataagtt tgtataatat taatttttga
     3661 tatataattg atattgtaag caatgttaaa cagctattgt aactatatct acgaatacta
     3721 ttatcattac tataattggg aacgctgcta tgaacaaata aatattctgt aaaggaataa
     3781 atattatgac tttaaaaaat tttcttataa aacataatta tttgataaag ctttctatta
     3841 gtttagtcat tgcaatctac ctgcctggga tttttatcgg acttggggtc attaaaagtt
     3901 catataacga aattgtaaat aaaagtgaaa ataaatatta tgatatgaca acatcctttt
     3961 ctgcatattt tattgaacag atcaatttct taaaaaaaca cgcacttaca ataatatatg
     4021 acaacgaaaa tgcacccgtt gaaaaaaaga ttatgaaaga tattattgat tcccatccat
     4081 attattattt aacaacaaca aaaatattat cggattatag aataggtttg cctaatgcaa
     4141 ttgatatagg aatctgcttt aaaggctcag attatattat tacttctaca tttaaatata
     4201 aaataagtga ttttatgaaa atatcttcca caaacaacac ttataacaca aaagaattag
     4261 aagaattttt taattatgat atagatagaa ttaaacttgc atcaaacttt aactgtagaa
     4321 atgtaacaga agcaaatttc cttgtgggag taccggtagt atttaataaa caacatgaag
     4381 ctttaattat ttacataatg agatatgatt cagtaaatac aacttttttt ggcaaagagg
     4441 agatagagaa tgtttcattg ctgattttta acgctgatgg ccaattagct tttgccagcg
     4501 acccaaaaag taatgacttt taccataata tatcaggaaa cgacgcattt agaaaatatc
     4561 tgcttaattc tgatacaaaa atagaaagtt tcacaatagg gtataatttt actgccatga
     4621 aggtatataa ttccaacatt agaaaaatat ttgtatcaat aatccctgaa aacttgatgg
     4681 aggaaaacct taaccagtat ttaagcatag tatataagac aacaacttta gccatgatgg
     4741 catttgcatt gttattaggc atatcggtat atattaatta taaacctatt tttaatcttg
     4801 ttaaacctat aagaaagaaa aatccaataa atctaagcag cgaaatcagg actatatcaa
     4861 cggccatgga taagatggaa aaaaacgttg ctgaacaaaa gatcctgtta atggattatt
     4921 tgctgggaaa tatcttgtat ggcattccta tatcagaaga aataatggat agactggatt
     4981 ttaataggag tagcgatttg tattgtgtat ttgttataat ggatataaaa atgaattccc
     5041 gcgaaagaga tagaataact aattatttat atagtaagtg caatatccac gcttatgtta
     5101 ctgacatttt gaataaaaat catataattc ttatatgcac ccttaagaat aatgatgttt
     5161 ctataatcct gagcgaatta aaaaagtatt taaatgagat atacgaattt gtttataaaa
     5221 taggagtggg acatatagtc aacaatatcg gcgatataag aaaatcctat ttgaacgctt
     5281 tacgcatagt agagcttaca atgattaatg taaatttact gggctcagaa atttctttta
     5341 cagaaaatta tcccacagaa gaaataaatt tattcttaca atacattgaa aatagtgaaa
     5401 aggaaaacgc cttcggcatt ctggataata tattcaaata cataaatagt atagaaatat
     5461 cattattaag gcaatatatt gcttgtgatt tacttatctc atatattaaa tgcttaaaaa
     5521 agataaaata tccctttgat aaaaaagatg agttcatgct ttttaataac aacagcactg
     5581 ctgaattgtt tgaagctatg cgtatttcag tatcaacagt ctgtgatgta atcctgcaca
     5641 ataatgaggt atcctactcc aatttgcaga aggagattat tgaatacgta aaccaaaact
     5701 ttactagctt tgatttgtgt agaacccaag tggctgatat atttaacatt tctatataca
     5761 cactgtcaag aatatttaaa gagaacgcag ggttaggctt taaggaatat gtcttatcaa
     5821 agcggttgga attatcaaaa gccttactta tgaatacgaa taaaagcata acccagatta
     5881 caattgaggt aggattttct gatccgggtt atttttcgcg gatatttaaa atgacatatg
     5941 gtaccacgcc tagtcaattc agatccggca atagcctgta agcgctgaaa ctttcaatta
     6001 ccaattactt tcttaatgag tcataaatag aaatgatgct aaactacaaa ttctgaggtt
     6061 tagcaccatt tttctctttt tattcctaaa gtcaggttac atgtgatttg tattatgttg
     6121 atgaattttg tcgaatcgta acgagtcatc tattctattt cgctttactg ccttattctt
     6181 tccactttcc tattcatact tccagcaacc caacttacac ccaagaatcc taatatacca
     6241 acactggacc cagtgctatc tataattaca tctctaattt ctcctgatct gccgggtata
     6301 aaaatctgat gcacttcatc tgatacagca tacaaaatgc aaatcaaaag ggccaataat
     6361 acacttctgt aaccacatac cccgcttctc ctcaaagcat ttaccaccag tatcccaagt
     6421 tcaaaataat aaaagaaatg agcgttcttc ctgactatat gatgaaggtc tccttttt
//
LOCUS       DAOSDD010000240         6460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4330_length_6460_cov_5.807338, whole genome shotgun sequence.
ACCESSION   DAOSDD010000240 DAOSDD010000000
VERSION     DAOSDD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4330_length_6460_cov_5.807338"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PK733_16275"
     CDS             complement(<1..1758)
                     /locus_tag="PK733_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ12130.1"
                     /translation="MEQRKTGTADIHYYSDRLKHKLNNLRTSHAAIVEAPSGYGKTTA
                     VRDYLEGNLSQSSAVYWFTAADEVPEAGFRRLCREIEKIDGRAGERLQRIGFPNAVTI
                     GEACEALRSIQCDRESWLVIDNFQSLNAILPPFFLTALLEHGGSGLRIVIVTQMLGRD
                     VHTLIAGRGFLHITTSDLRLEAGDILRYYALAGVKITREEAQSVVHYTEGWIIAVYLQ
                     LRAFQETGAFSDTAIISLMDHLVWERLTEQQQMFLLHLSPFETITIQQACTLARFDVL
                     PGYALAALQSPSIRYERAGSRYEMHSILSELLAQKRRERGEVFERECFIHAGDLCRDN
                     NRNAEALALYWHIKDYRGILSLDLSLLIFDEIEDTPFSTIALDLTQNCPTEIRKEYPL
                     SMLRVAWALKAAGMDVAFSNLLDGLELTIGQDVSLQAEWLLLSAYRFYPHVDKMTPLL
                     KKAALLFDGKSSRVILPDAPWCFGNYSQLAVFHHKPGEADWEADTLEEFISLYSKLTG
                     GHGSGADTLFRAELAHYRGDLNQAEILAYKAIFLAEGNRQSIVQLGATMHLCEIAVEK
                     SSHEDWQSALYSMEHAASFR"
     gene            complement(2076..2708)
                     /locus_tag="PK733_16280"
     CDS             complement(2076..2708)
                     /locus_tag="PK733_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12131.1"
                     /translation="MVFTRNKILCNEIKHHTLSNVLPCFAVFLLIYILFGIEGLKKDT
                     VLIIAERFLPLMAIILLTPCFEYELDKGINQVIRSKTIYIGETYFIRMIWRIVLYVLS
                     TLMLMQFLKTQRAHMEFSLYTLESISIGLFLGSLGFLISGISSSLIGGYLVALVYYLI
                     QWMPNLKMLGAFYLFRIGKNLEPMIELNIGITLLFLISGLLGKLMIERRR"
     gene            complement(2689..3906)
                     /locus_tag="PK733_16285"
     CDS             complement(2689..3906)
                     /locus_tag="PK733_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12132.1"
                     /translation="MRLMLLEMKRILKNPLFWISIALIFVFIYSQFGSGIDISDMDKP
                     LPNQENYGITQTNNMSIIREKAIAQLLQEYSFNSYTTYPYGFYRQVELNDKKNLQMAK
                     IISAISGTSIEEIQEKQKKQQSSLMNGEQIQHIIIAKAQDSISAQEFLDLMDKADELL
                     GGGSFYSKKLIASQFGKRSKTYEEAIEDYELMINKDRVSGAYARLFCDYAGIIIGIIP
                     IFLAVAIWFKDQRSKCQDLLFTRKTSSLKFIISRYLAMLILFSIFLIVMGSYYNIQII
                     KEFGLSKINPFYFYGYILIWLIPILAVLLSIGTFTTIATNSPSGILVMMTWWFLDVMN
                     GLPGIKGGYGNSLILRHNIVGNTQIYLNNRAFIFINRGAYILFAIVILVISMSIYNLK
                     REGKVFVRGIHKK"
     gene            complement(3906..4784)
                     /locus_tag="PK733_16290"
     CDS             complement(3906..4784)
                     /locus_tag="PK733_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12133.1"
                     /translation="MDGKIKIKGLNAAYGQNQVLFDINLEIGKGMFGLLGRNGAGKST
                     LMKTLVTLLPIKNGEVEICNIPISRQREIRSMVGYLPQDFNMYPNMSVEESLEYLGVL
                     SEIKSSEIKYLTNKVLELVNLWQDRKVKVKALSGGMKRRLGIAQAIIHEPKVLIVDEP
                     TAGLDPEERIRFRNLLADLAQDKIVILSTHIVGDIESTCNHLAVLNHGKIIFNGEVSQ
                     LIAGAKGYTYKVQIPKNRFHEYRNRYVLTAQQDLGEYIDIKLLSKGKVEEGFVPCEPN
                     IEDGYLNLLYDVGGVK"
     gene            complement(5044..6315)
                     /locus_tag="PK733_16295"
     CDS             complement(5044..6315)
                     /locus_tag="PK733_16295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ12134.1"
                     /translation="MDWLNEWTKKLSLRKSFTIYLFTFFLLGISLAKVNTILINSYEI
                     KSSDNQMVEVSLIKDDEIKTYYYLKAFNHKKTLPDSNLFKLIHKSLLLYRDYAVEIHC
                     LLFAILAFAVFYRHKLQKPIGYLNSYSNNNSCSSNINDNNNNDDDESNDIAVTLPEGE
                     NELCKACEKINIYTRDLHEDKIKIWRQYDAFNHMISSIGHDIRTPLTVLKGNQEMMWI
                     FEEENSESNMSKISNISNISNIPDRSKNKRLELLASMERQTQRIQEYVEKIKNLQSMD
                     KLVIKKCEISLKDFIATLEKTINALPDNKRIHWTKLQEDRTIFIDSIHIQEVFENIMS
                     NAIRFASKNVYIEFCVTDKELKIRIQDDGPGFSKEALSYATSAFFCENPTDGHMGLGL
                     NISSIIIEKHGGKLLINNTKCGGTAEIIINL"
     gene            complement(6303..>6460)
                     /locus_tag="PK733_16300"
     CDS             complement(6303..>6460)
                     /locus_tag="PK733_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOJ12135.1"
                     /translation="EHIYTSIWGYDGIGDNSVIAEHVRRIRLKLSQYTDKEYIETVWG
                     VGYRWIG"
BASE COUNT         1947 a         1371 c         1053 g         2089 t
ORIGIN      
        1 tcgaaaggag gctgcatgtt ccatagagta cagcgcactc tgccaatctt catgactgga
       61 tttttccacg gcgatctcac acagatgcat cgtcgcgcca agctgcacaa tactctgccg
      121 gttgccctca gcaagaaaaa tcgccttata tgccagtatt tctgcctgat tcaagtcgcc
      181 ccggtaatgg gcaagctccg cacgaaagag cgtgtccgca ccactgccat ggccgcccgt
      241 caacttggaa tacagcgaaa taaattcttc gagcgtgtct gcttcccagt cagcttctcc
      301 tggcttgtgg tggaacacgg ccaattgcga atagtttccg aaacaccacg gagcgtcggg
      361 taaaataacc cgtgagcttt tcccgtcaaa caaaagcgcg gctttcttca acaagggcgt
      421 cattttatca acatggggat agaaccggta agcggataac agcagccatt ccgcctgcaa
      481 cgaaacatcc tgcccaatcg ttaattcaag tccgtccaat aagttggaaa acgccacatc
      541 catgcccgcg gctttgagcg cccaggcgac acgcagcatg gaaagaggat attcttttct
      601 gatttcggtc gggcaatttt gcgtgagatc cagtgcaatc gtcgaaaagg gcgtgtcctc
      661 gatttcatca aagatcaaaa gggataaatc aagggagagt attcccctgt agtctttaat
      721 atgccaataa agagcaagtg cttccgcgtt cctgttgtta tcccggcaca ggtcgcccgc
      781 gtgaataaag cattcacgct caaacacctc accacgctcc ctgcgtttct gcgcgagcaa
      841 ttcggatagg atgctgtgca tctcgtaccg tgatccggcc ctttcatacc ggatagatgg
      901 gctttgtaac gctgccagtg catatcctgg taacacatca aagcgggcga gagtgcacgc
      961 ttgctgtata gtgatcgttt caaacgggga caggtgcagc agaaacatct gctgctgctc
     1021 cgtcagcctc tcccatacca ggtggtccat caaagaaatg atcgccgtgt cagaaaaggc
     1081 tcctgtttcc tgaaatgccc ttagttgcag atacacagcg atgatccagc cctcagtata
     1141 gtgaacgaca ctctgagcct cctctcgggt aatcttcacg cctgcgagcg cataatagcg
     1201 caggatgtcc ccagcctcca accgcaggtc ggaagtggtg atatgcaaaa aaccccggcc
     1261 ggcaatcaac gtatggacat cccgccccag catctgcgtc acaatgacga ttcgcagccc
     1321 cgagccgcca tgttccagca gtgcggttag aaaaaaaggc ggcaggatag cgttcagaga
     1381 ctggaagttg tcgatcacga gccagctttc ccgatcacac tggatggacc gcaacgcctc
     1441 acaggcttcc cctatggtga cggcattggg aaaaccgatt cgctgcaaac gctctcctgc
     1501 acggccgtct attttttcga tctcgcggca aagccgccga aagcctgctt ccggcacttc
     1561 gtctgcggca gtaaaccagt acacagccga actctgcgag agattgcctt ccagataatc
     1621 ccgcacagcg gtggtctttc cgtagccgga tggagcttcg acaatagcgg cgtgcgaggt
     1681 tcgcaaatta ttcaatttat gtttcaaacg gtccgaataa tagtgaatat ccgccgttcc
     1741 ggttttcctt tgttccatct cattgccccc tttgcgctgt ggttctgctg tgggttgtat
     1801 ggcttttccg cgtccttctg aattcttaag atagtttgaa ggtgtaagca gtattctagt
     1861 tttttatagt agaaggaatt tcatatgctg ttgaacaata cattatcctt aactaattgt
     1921 tttaaatatt ctgttttttt catgtttata ttctactgga aatattagta ttattcaacc
     1981 ctatcctttg aaatcagcta tctttaaaat ttcaaaacca ccatagtttc ctatacgtgg
     2041 aaaaaagcca gtttcactga tttagagtaa atctcctacc ttcttcgttc tatcataagt
     2101 tttcctaaaa gtcctgaaat taaaaacaat aaagtaattc caatatttag ttcaatcatt
     2161 ggttcaagat tttttcctat tctaaacagg taaaaagctc ctagcatctt caaattaggc
     2221 atccactgga ttaaatagta taccaaggca accaggtatc cacctatcaa actggaacta
     2281 atccctgata ttaaaaaacc taaagaacct aaaaacagcc ctatagatat agactccaga
     2341 gtataaaggg aaaactccat atgtgctctt tgggttttca gaaattgcat taacatcaat
     2401 gttgacaata catataaaac aattctccat atcatacgga taaaatatgt ttcacctatg
     2461 tatatagttt ttgaacgaat cacctgatta atgcctttgt ctagttcata ttcaaaacat
     2521 ggtgttagta aaataatcgc cataagaggc agaaatcttt ctgctataat aagtactgta
     2581 tctttcttta gtccttcaat gccaaataat atataaatta gtagaaaaac agcaaaacac
     2641 gggagcacat tagataaagt gtgatgctta atttcgttac ataaaatttt atttcttgtg
     2701 aataccacga acaaaaacct tcccttcccg ttttaaatta tatattgaca tggaaataac
     2761 tagtattact attgcaaaca atatgtatgc accacgattg ataaaaataa aagccctatt
     2821 atttaagtaa atttgcgtat tgccaacaat attatgacga aggattaaac tattcccata
     2881 tcctccctta atacccggta atccattcat tacatccaga aaccaccaag tcatcatgac
     2941 taaaattcct gaaggagaat tagttgctat tgtagtaaaa gtgcctatag aaagtaagac
     3001 cgccaatata ggtatgagcc aaatcaatat atatccatag aagtaaaaag gatttatctt
     3061 acttaatcca aattctttta taatttgaat attataataa ctacccataa ctattaagaa
     3121 aatactaaac agaataagca tagccagata tctgctaatt ataaatttta gcgaagaagt
     3181 tttccttgta aacagtaaat cctggcactt acttctttga tctttaaacc atatggcaac
     3241 agccaagaat attggtatta ttcctataat gattcctgca taatcacaga ataatcttgc
     3301 ataggctcct gataccctgt ccttattaat catcagttca taatcctcta tagcctcttc
     3361 atatgttttt gatcttttcc caaattgtga agcaattagc tttttgctgt aaaaggagcc
     3421 tccccccagc aattcatctg ctttgtccat caaatccaga aattcttgag ctgaaatact
     3481 atcttgagct tttgcaatta taatatgttg tatttgttct ccattcatta aggaagactg
     3541 ttgttttttc tgtttttcct gaatctcctc tatggatgtt ccggaaatag cagaaatgat
     3601 ctttgccatt tgcagatttt ttttgtcatt taattctacc tgcctataaa aaccataggg
     3661 atatgttgta tatgaattaa aagaatattc ctgcaacagt tgagcaattg ctttttctct
     3721 aataattgac atattatttg tttgtgtaat accataattt tcttgattag gtaaaggttt
     3781 atccatatca gatatatcaa tcccacttcc aaattggcta taaataaaaa caaatattaa
     3841 tgctattgat atccaaaata atggattttt taatatccgt ttcatttcaa gaagcattaa
     3901 tctcattatt taacacctcc tacatcataa agaaggttca ggtagccatc ttctatattt
     3961 ggctcacatg gaacaaatcc ttcttccacc ttgccttttg ataaaagttt tatatctata
     4021 tattcaccca aatcttgttg tgctgttaaa acatagcggt ttctatattc atggaatctg
     4081 ttttttggta tttgtacctt gtaggtatat ccttttgcac ctgcaattaa ttggcttact
     4141 tctccattga agattatctt tccatgattt aatacagcta aatgattaca ggtactttca
     4201 atatctccta caatatgagt ggataaaatg acaattttgt cttgagccaa atcagctaaa
     4261 agatttctga aacgaatacg ctcctcagga tccaatcctg ctgttggttc atctacaatt
     4321 aaaaccttag gctcatggat aatggcttgt gcaattccta agcgtctttt cattccgccg
     4381 gacaatgctt ttacctttac ttttctgtcc tgccagagat taactaattc cagtacctta
     4441 tttgtaagat attttatttc agaggatttt atttcagata aaacacccaa atattccaat
     4501 gattcctcca cagacatatt gggatacatg ttaaaatcct gaggtaagta accaaccatg
     4561 gaacggatct ctctttggcg ggaaattgga atattacaaa tttcaacctc cccatttttt
     4621 ataggcagca aagtcaccaa agttttcata agtgttgact tacctgcacc atttctcccc
     4681 aacaacccaa acataccttt accaatttca agattgatgt caaagagtac ctggttttga
     4741 ccataagcag cgtttaaacc tttaatttta attttaccat ccatattaag ctccattcct
     4801 accgcgtgcg aaatgaattc gcccttgcag gaacaaaact tttatgaaag tttttatggg
     4861 cttaaattca tttgagctgc gcgtgcaatt tattttttat ttctaatatg tgcaaaaaat
     4921 atgcacatgc gcattagttt tacagaaatc tacaatttcc ttcaaactaa aacaccatcc
     4981 ttattaattt aaagatagtt tattatataa tttccaatat tttttcaagt tttataattt
     5041 attttataaa ttaataatta tttctgctgt tcctccacat ttcgtattat ttatcaatag
     5101 tttacctcca tgtttttcaa tgataatact ggaaatatta agccctaatc ccatatgacc
     5161 atctgtcggg ttttcgcaga agaatgcaga agttgcataa cttaatgctt ctttactaaa
     5221 tccaggacca tcgtcctgaa tccgtatctt taattcttta tccgtgacac aaaactcaat
     5281 atagacgttt ttacttgcaa aacgtatagc attgctcata atattctcaa aaacctcctg
     5341 gatatggatg ctatcaatga aaattgttct gtcttcctgt agcttggtcc agtgaattct
     5401 cttattatca ggcaaagcat ttatagtttt ttccaaagta gcaataaaat ctttaagaga
     5461 aatttcacat ttttttatta caagcttatc catggactgc aaattcttaa ttttctctac
     5521 atattcctgg attctttgcg tctgcctttc catagaagcc aaaagctcca gtcttttatt
     5581 tttagacctg tcaggtatat tagatatgtt tgatatatta gatatttttg acatatttga
     5641 ttcggaattt tcctcttcaa aaatccacat catttcttga tttcctttta aaactgtaag
     5701 tggtgtccta atatcatgac cgatagaaga aatcatatga ttaaaggcat catattgacg
     5761 ccagattttt attttatctt cgtgcaaatc cctcgtatag atattaattt tctcacaagc
     5821 cttgcatagt tcattttcac cttcaggtaa agtcacagca atatcattac tttcatcatc
     5881 atcattatta ttattatcat taatattact gctacatgag ttattgttgc tatatgaatt
     5941 aagataacct ataggttttt gaagcttatg cctgtaaaaa acagcaaaag ctaagattgc
     6001 gaaaagtaaa caatgaattt ctactgcata atctctatat aataatagag atttgtgaat
     6061 taacttgaat aaattggaat caggcaatgt tttcttatga ttaaatgctt ttaggtaata
     6121 ataggttttt atttcatcat cctttatcaa agatacctct accatttgat tatctgatga
     6181 ctttatttca taagagttta tcaatatggt gtttactttt gctaatgaaa tccccagtaa
     6241 aaaaaaagta aacaaataga tggtaaaact ctttctcaaa ctcaattttt ttgtccattc
     6301 gtttaaccaa tccatctata acccactccc caaactgttt caatatattc cttgtctgtg
     6361 tattgagata attttaaacg tattctgcgt acatgctctg caatgactga attatcacca
     6421 ataccatcat acccccaaat tgatgtgtag atatgttcct
//
LOCUS       DAOSDD010000241         6384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4383_length_6384_cov_4.825407, whole genome shotgun sequence.
ACCESSION   DAOSDD010000241 DAOSDD010000000
VERSION     DAOSDD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_6384_cov_4.825407"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PK733_16305"
     CDS             complement(<1..910)
                     /locus_tag="PK733_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOJ12136.1"
                     /translation="MKFIIPIIVGAIIGYITNWFAIKMLFRPHHEIKILGVHVPFTPG
                     LIPKEKSRIAKSVGETIGVHLLSPKIVTEALSIDKINGHLQIWIKNNLGKLCESDKTI
                     GDLATGLFGNDCNKLLNAIKVRLADAVCSQLRELRTKNKLMNILDTKVYDKYSDDFYK
                     TVKQKAELFLYELTASEEIKAGLRDALTAKIKELEHDTRALDEVIPESVVNAVKEYIN
                     DHSGEIIKAIKEILQNPSVKRRIKDSISEVVSQNTSKLVTMFMSPESIAEKVFVAIEK
                     YIDNPENNKSIISIIISATEKIMKGKV"
     gene            1585..3501
                     /locus_tag="PK733_16310"
     CDS             1585..3501
                     /locus_tag="PK733_16310"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOJ12137.1"
                     /translation="MKLLKLLKDLVSGLYASVKRFPVTIGLTSAVSILLIIITQNESN
                     FTNELRDSLNRIAMILTLGAPLSLCIKLAFEKYENVHLVYKATKDREKANQQVSDNAP
                     SEITSHKIIAPKTIAPKTIAPKTIAQKAIVYRILAYIFGAGALVLYYFFLLPNFSMVS
                     ISRYLAFSIALYLGFFFIPYLPNRSDFELYVIKILSRFFITLIYSAVLYGGLAAIMFT
                     LDKLLSVPIQSRYYYYVWLIIAGVFAPSYFLAGLPASANGLHVSSYPKALKILLLYIV
                     MPIITAYTLILYTYFAKIIITLQWPVGLVAHLVIWYSVISTGVIFLVSPLVNENKWVK
                     NFTFWFIKLIIPVMIMMFVAIGIRLKAYGITENRYFLVVLGSWVLGIMIYISFTNAKK
                     NIILPVSLALIAVLAVSGPWSSYSVSKFSQNKRLENILTRNGMLKDDQIMKNGIEVPD
                     TDKIEISQIITYFERNHKLSDVKYLPSDFKMENFEYVLGFPYTYNAYERPAEKFFAYS
                     SYDWSKPVDIGGYDYLIDSRGLYSETELIVDSMRINYDFNSTSFRILYGDDEIYGKTL
                     SDFGTQLFYKYGTTEKQKVNPDDMYFVEENDKVKLKFVFMSVYGEKDSSAGNARISSM
                     EFYILIKLKGAPTQ"
     gene            complement(3515..5161)
                     /locus_tag="PK733_16315"
     CDS             complement(3515..5161)
                     /locus_tag="PK733_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12138.1"
                     /translation="MFAKYHKSIIIVITIIILTIFVSILMILTSCSGLNTYNHSNDVD
                     NDIDYISGSDSDNDSNNPYYHVWGDKSNWCAVKGAMPDSYIRLDDINKYSVPEIKLQF
                     DIGMYGNVIRETTDGYLYGNMLSLDEKSSDSWDAEKVPKIRKLDKNGKVIWEKIYDYK
                     TYSGQLNNLLVYHDDSFLFSVNTYPRTKDNQMVYEKSYIIKCDKSGTELWKSDFDDYS
                     GSLFQNIFITSSNEIIIVGQCYSKAGRQTKGDASQANELNGDASQANELNGDASQTNE
                     LNGDASDDIVVIKLDKSGNILNQKSFGGSDFDNLYNARYDEEIGIIINGRTQSKDGDF
                     AANTKEVEYNNVEFVACIDNDLNLKWVVHENDKKKFITMPPAISEGFIYVVGGHVSGI
                     AAGSGSNAAPDGFLMKLDKNGKKIFEKTHLFNNYWGQTLSVLNNGDIVIGTGKQNQGI
                     IIIIDKYGNVKKELEDIKFSPGNIVPTEDGGFITTAHRIIKTVPQPPVISSIWYDTEL
                     VVVKYGSNYSIEWRKTYNKYKDEMGFDFVIPFKTGKVIIE"
     gene            complement(5225..5434)
                     /locus_tag="PK733_16320"
     CDS             complement(5225..5434)
                     /locus_tag="PK733_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12139.1"
                     /translation="MTKEESDILKKVIDEKKETDETVYAVGIAIRDKMEEMKKQGQEE
                     CIKEGIEEGIEEGIEKGRVEGRAEG"
     gene            complement(5523..5948)
                     /locus_tag="PK733_16325"
     CDS             complement(5523..5948)
                     /locus_tag="PK733_16325"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOJ12140.1"
                     /translation="MVYKVKVNGREVFYYILLEPQSTVDYKIPYRLLQYMLEIWRTIL
                     KDTGEKESASKDIKLPAIVPCVCYNGCYNWTASGCFSETLASNELFGDFLLNFKYIPF
                     DVARYKEQELLQLSNLIGVVFYIDQKLQYDEMIRRLENW"
BASE COUNT         1930 a         1218 c          953 g         2283 t
ORIGIN      
        1 atactttacc tttcattatc ttttctgtag ctgatataat aatcgatata atacttttat
       61 tattttccgg attatcaata tacttttcta ttgctacaaa taccttttct gcaatagatt
      121 cagggctcat aaacatagtt actagtttac ttgtattttg ggaaacaacc tcagaaattg
      181 aatctttaat tcttcttttt actgacggat tctgaagtat ttcttttatt gcttttataa
      241 tttctccgct atgatcattt atatattctt taacagcatt aacaacactt tctggaatca
      301 cttcatctaa agctctcgta tcatgctcta attcctttat ttttgcagtt aaagcatctc
      361 ttaaccctgc tttaatttcc tctgatgcag ttaactcata tagaaacaac tctgcttttt
      421 gtttcacagt tttatagaaa tcatcactat atttgtcata tacttttgta tcaagtatat
      481 tcatcagttt atttttagtt cttagctccc ttaattgaga acaaacagca tctgccagtc
      541 tcacctttat cgcgtttaat agtttattac agtcattgcc aaaaagacca gtggctaggt
      601 ctcctatggt cttatcactt tcacataact tgcccagatt gtttttaatc catatctgca
      661 gatgtccatt aattttatca attgatagag cttctgtaac tattttaggt gataaaagat
      721 gaactcctat ggtttcccct acactctttg ctattctgct cttttccttt gggataaggc
      781 ctggtgtaaa tggtacatgc acacctaaaa tttttatctc atgatgaggc ctaaatagca
      841 tctttattgc aaaccagttg gtaatatatc caataatcgc accaacaata attggtatta
      901 taaatttcat atttcaacct ctaatcttaa tatagtacat tacagcacat tacagcacat
      961 tacattacag tacagtacat tatagtacat tatagtatag tacaaaactg ttctgttctg
     1021 tattaataat aaataatata actttaatat agttttagca caaaatcaaa ttagtttcaa
     1081 atacaaattt ttactgatgg gtacatttac tgagtttgaa gaaatattta caaggggctg
     1141 tcgttacagt gcttagagcc tttaaattca gctatgggca aaatcaaact cacaaaataa
     1201 tctaaataaa aggattgtaa tattttgttc aaacactgat tttgctctcc cttcgcttag
     1261 atacggggaa acccttcagc atgaaaggaa tacctgttac ttgagagaag agtttcccct
     1321 gcctagctac agcatcctcc acttatgccc cttaccaata ttttctttaa ataaattaaa
     1381 tgtacccctt actaattgaa aacttcaaac ttgaacttga gaagtgtccc cataattata
     1441 gagaacttga gaagtgtccc cataattata attataatca gagaagtgtc cccataattg
     1501 gacaagtgac ccgtatttgg tcttttttct cattgatttg ttgtgtaaaa catagtaata
     1561 tggtggagaa aggcgggaac tgatatgaaa cttttaaaat tacttaaaga ccttgtatcc
     1621 ggtctttatg caagtgtcaa acgttttccc gtgactatag ggttaacttc agcagtttcg
     1681 attttgctaa taattattac acaaaatgaa agtaatttta cgaatgaatt aagggacagt
     1741 cttaaccgta ttgccatgat actgaccctg ggggcaccgc tttctctttg tatcaagctg
     1801 gcttttgaaa aatatgaaaa tgtacattta gtctataaag ccaccaaaga tagagaaaag
     1861 gcaaatcagc aagtgtcaga caatgcacca tctgaaatta catcgcataa gattatagcg
     1921 cctaagacca tagcgcctaa gactatagcg cctaagacta tagcgcaaaa agctatagta
     1981 tatagaatat tagcgtatat ttttggagca ggagcgcttg ttttatacta tttctttttg
     2041 ctgccgaatt ttagtatggt ttctatttcc aggtatttgg cttttagcat cgcactttac
     2101 cttggatttt ttttcattcc ataccttcct aatagatctg actttgaatt atatgtaata
     2161 aagattctct cacgcttttt tattacgctt atatattcag cagtgcttta tggagggctc
     2221 gcagctataa tgtttacatt agataaactg ctgtctgtac ctatacaaag ccgctactat
     2281 tactatgtct ggcttatcat cgcaggggtt tttgctcctt cctacttcct ggccgggctc
     2341 ccagccagtg ccaatgggct tcatgtttcc agctacccaa aggctctcaa gattcttctc
     2401 ttatatattg tgatgcctat tatcaccgca tatacactga ttttatatac gtattttgct
     2461 aaaataataa ttactttaca atggcctgta ggtcttgttg ctcatttggt aatctggtat
     2521 tctgttataa gcacaggtgt tatttttctt gtttctcctc tggtcaatga aaataaatgg
     2581 gtaaagaatt ttacattttg gtttattaaa ctgattatac cagtcatgat aatgatgttt
     2641 gttgcaatag gtataagact aaaagcatat ggcataacgg aaaacaggta ttttttagta
     2701 gttctgggat catgggtatt gggtataatg atttatataa gctttaccaa tgctaaaaag
     2761 aatatcatat tgccggtttc cctggccctt attgcagtat tagcagtgtc aggtccatgg
     2821 agtagctact cggtatctaa atttagtcag aataaaagat tagaaaatat tttaacaaga
     2881 aatgggatgc tgaaagacga ccaaataatg aaaaatggta ttgaagttcc tgatactgat
     2941 aaaattgaga taagccaaat tataacatac tttgaacgta atcataaatt aagtgatgtt
     3001 aaatatcttc ctagtgattt caaaatggaa aatttcgaat atgtactcgg ttttccatat
     3061 acctataatg catatgaaag gccggcagaa aagttttttg catatagttc atatgattgg
     3121 tcaaagccgg ttgacatagg aggttatgat tacctgatag actctagagg tctatactca
     3181 gaaactgaac taatagttga tagcatgcgt attaattatg attttaatag taccagcttc
     3241 cggattcttt atggtgatga cgaaatatac ggcaaaacct tgtctgattt tggtacacag
     3301 ctattttaca aatatgggac aactgagaaa cagaaagtaa atccggacga tatgtacttt
     3361 gttgaagaaa acgacaaggt taaattaaaa tttgtgttta tgagtgtata cggagaaaaa
     3421 gattcctccg caggtaatgc tagaattagt tctatggagt tctacatttt gattaaatta
     3481 aaaggcgcac ctactcagta ataatcttga gcacctactc aataataacc tttccagttt
     3541 tgaagggaat gacaaaatcg aaacccattt catccttgta tttgttatag gttttgcgcc
     3601 actctattga ataattgctt ccatatttaa ctacaacaag ctctgtatca taccaaatag
     3661 aagatataac aggcggctgt ggtacggttt ttattatccg atgtgcggtt gtaataaaac
     3721 caccatcttc agtaggtaca atattaccag gagaaaactt tatatcctca agttctttct
     3781 taacatttcc atatttgtca ataataatta ttattccttg attttgcttt cctgttccta
     3841 tcactatatc accattattt aatacagata atgtctgtcc ccagtaattg ttgaacaggt
     3901 gtgttttttc gaatattttc tttccatttt tatcaagctt cataaggaat ccatcaggcg
     3961 cagcattact tccgctacca gcagcaatac ctgaaacatg accacctaca acatatataa
     4021 agccttctga tatggcaggt ggcatagtaa taaatttctt tttatcattt tcatggacaa
     4081 cccacttcaa attaaggtcg ttatctatac aggcgacaaa ttccacgtta ttatattcta
     4141 cttccttggt gtttgcggca aagtcaccat ctttagactg agttctgcca tttattatta
     4201 tcccaatctc ttcatcgtac ctggcattat aaagattatc aaaatcactt cctccgaaac
     4261 tcttttgatt caatatattc cctgacttat caagttttat aacaactata tcatcagaag
     4321 cgtccccatt tagttcattt gtttgagaag cgtccccatt tagttcattt gcttgagaag
     4381 cgtccccatt tagttcattt gcttgagaag cgtccccctt tgtttgccga ccggctttac
     4441 tgtagcattg gcccacaata ataatttcat tactgctagt tatgaagata ttttggaaaa
     4501 gagaacctga ataatcgtca aaatcacttt tccaaagctc agtcccagat ttatcacatt
     4561 ttattatgta actcttttcg tataccatct ggttatcttt tgttcgggga taagtgttta
     4621 ctgaaaataa gaagctatca tcatggtaga ctaataaatt gttcaattgt ccagaatagg
     4681 ttttataatc atatatcttt tcccaaataa ccttaccatt cttatcaagt ttccgaattt
     4741 taggaacttt ttccgcatcc catgaatcag aagatttctc atcaagactt aacatatttc
     4801 catataaata accatcggta gtctctctta ttacattgcc atacattcct atgtcaaact
     4861 ggagttttat ttcaggtact gaatatttat ttatatcatc aagtcttata taggaatcgg
     4921 gcatagcacc ttttactgca caccaattgc ttttatctcc ccaaacatgg taatatggat
     4981 tattactatc attatcacta tcactaccac taatataatc gatgtcatta tcaacgtcat
     5041 tgctgtgatt ataagtattt aaaccggaac atgaagtcaa tatcatcaat atcgatacaa
     5101 atattgttag tatgatgata gtaataacta taataataga tttgtgatat tttgcaaaca
     5161 taattctatc ctcccactta gcccatgcgc agtaagtttt tgctatctct atttttgcat
     5221 ttttttatcc ttcggccctg ccctcaactc tgcccttttc aatgccttct tcaatgcctt
     5281 cttcaatacc ttctttaatg cattcttcct gaccttgctt tttcatctct tccatcttat
     5341 cccgtattgc tatcccaaca gcataaactg tttcgtctgt ctccttcttc tcatctatta
     5401 ctttttttaa tatatcagat tcttcttttg tcatcccatg cgttgcaacc ttttcaaact
     5461 atgttctaaa cagttcaaaa tcttcttcgc tcatcttctt tcgctcatct tctttaatag
     5521 atctaccagt tttccaacct tctaatcatt tcatcatatt gcagtttttg atctatgtaa
     5581 aacacaacac ctattaaatt gctaagctgt aataattctt gctctttata tcttgctaca
     5641 tcaaagggta tatatttaaa gttcagcaga aaatcaccaa ataattcgtt tgacgcaagc
     5701 gtttcactaa agcagccact tgctgtccaa ttgtaacatc cattataaca tacacatggt
     5761 acaatcgcag ggagcttaat atctttgctt gctgattctt tttctcctgt atcctttaga
     5821 atggtcctcc agatttccag catatattgc agtaacctgt atgggatttt gtagtctaca
     5881 gtactttggg gctccaataa aatgtagtag aatacttctc ttccattcac tttaacttta
     5941 tataccaggt ctacttcctt cccttaaaaa tcatagatca tatgttatat caggctctag
     6001 tacccatagg tactccatga tttcacttcc attttacaat attttgattt gattttcaat
     6061 acattgcttc aatacgggtg catttacttt ctatgataca aaatatagaa aatgcacccc
     6121 cttcaataca ttgcatcgaa acaacgataa ccttcaatat acaaatacca atactattaa
     6181 ttgttgtatt tggtgctgtt gtatttggtg ccaggctaaa aacacaacgg aaccgtttta
     6241 tttttaacct ggcatcatac ttaaataaat gatattatat gtttatggaa tatgtgtaca
     6301 taggagggat tctattggca agaacaagca ttaaaccttc gttcggtgag gaagttggaa
     6361 acgctataac tcatggtgta gcat
//
LOCUS       DAOSDD010000242         6342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4433_length_6342_cov_4.761094, whole genome shotgun sequence.
ACCESSION   DAOSDD010000242 DAOSDD010000000
VERSION     DAOSDD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4433_length_6342_cov_4.761094"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1102
                     /locus_tag="PK733_16330"
     CDS             152..1102
                     /locus_tag="PK733_16330"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12141.1"
                     /translation="MKNIISFESLDREEYSHIPLLKMSDNNHGGLPIFVRKYTLNSCN
                     KGQLHRHEFMQINYVCQGKGGHYINKQEFKIIKGDIFVIPPYIPHTIKTDEDSNIQIF
                     EFEFVPEFVNQDFESIANIESFFDFAYIEPFLVSESQVKPRLNLVGKIQNEVENILTE
                     VLSEYNEKKPGYLLVIKSLLLKLLVMVGREFTRDLENSESRTAFDRHRDSIFGAIKYI
                     DEHFAEDLNVEEVAKKFMLSQSYFSYLFKSITSKTFTEYVNDIRISKAMELLKDSDKR
                     VLEICYEVGFNNVNHFNRLFRQQTGISPLAYRKQNPEKHN"
     gene            1527..2858
                     /locus_tag="PK733_16335"
     CDS             1527..2858
                     /locus_tag="PK733_16335"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ12142.1"
                     /translation="MAEKLALFGGPKSVQSDAGDMFVWPIITKEDEDAVLDVLHKRAM
                     SGTSITRQFEKEFAAWQGTRYALGFSSGTAAIQTAMYACKVGIGDEIIAPSVTYWASA
                     TQAYSLGATPVFAEIDTDTLCIDPKDIEHRITDRTKAIVVVHYLGHPADMDPIMEIAR
                     RHNIKVIEDVSHAQGGLYKGRKVGTIGDVGAMSLMSGKSLAIGEGGMLVTDDVEIYER
                     SLAFGHYEMFRADEIQTEYLKPLAGLPLGGYKYRMHQMSSAVGRVQLKYYDQRCEEIR
                     KGMNYFWDLLEGVPGLKAHRTSEDSGSNMAGWYAPHGIYKADELEGLSVTRFTEAVRA
                     EGVESCYPGCNAALHTHALFNTADVYGHGKPTRIANSNKDVREFDKSLPISEKIGSMI
                     YAIPWFVRYRPEIIEQYAKAYRKAAENYKELLKDDPGNPPRLGGWHFFRHR"
     gene            3692..3767
                     /locus_tag="PK733_16340"
     tRNA            3692..3767
                     /locus_tag="PK733_16340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3725..3727,aa:Val,seq:gac)
     gene            complement(3810..4019)
                     /locus_tag="PK733_16345"
     CDS             complement(3810..4019)
                     /locus_tag="PK733_16345"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12143.1"
                     /translation="MTAKQYTKDYKTEAVKLMRKIGNTKAAVELGVPKNTLSQWVKQT
                     EIDDMDTSAGIQTPGSAMTQATEIQ"
     gene            4475..4699
                     /locus_tag="PK733_16350"
     CDS             4475..4699
                     /locus_tag="PK733_16350"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOJ12144.1"
                     /translation="MSTVTVRLNEEEAKIFNEYSKLHGVPLSTLFKKTLEEKMEDELD
                     MQVIKEYEKSLENGYTETFTHEEVKKMLGM"
     gene            4702..4974
                     /locus_tag="PK733_16355"
     CDS             4702..4974
                     /locus_tag="PK733_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOJ12145.1"
                     /translation="MKYQVLYTKKAIKQLSKIDVNQRHIILAWIEKNLVGIDNPMIYG
                     KALKGNLRDYWRYRIGDYRIITEINDDEIKIIVVGIGHRKDIYERL"
     gene            complement(5178..6005)
                     /locus_tag="PK733_16360"
     CDS             complement(5178..6005)
                     /locus_tag="PK733_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12146.1"
                     /translation="MIEVVPINRLNLTDLNMYQCGTEKCKPGHFYGPAVRDHFLIHYV
                     TEGNGVFQVGNTTYKLSKGQGFLICPGIVTFYQADIGLPWHYTWIGFHGLKAEDYLKQ
                     ANLSWDNPIFTYTKDDYIETCFKEMIETKNIKNGREMKLLGLFYSFLSKLIEENEQAS
                     SYNVTVNRKEVYIKKLIEFIELNYSRKISVSEIAAYLGLDRSYMGSIFKEFFNTSPQN
                     YLIDYRINKACDLMANYNLTIGDISRSVGYEDPLLFSKMFKKIKGLPPREYRKRLPL"
     gene            6194..>6342
                     /locus_tag="PK733_16365"
     CDS             6194..>6342
                     /locus_tag="PK733_16365"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOJ12147.1"
                     /translation="MKKIAFIGAGSFGFTRSLVRDILSFPAFADCTLALMDIDDERLS
                     YIKKAV"
BASE COUNT         2163 a          941 c         1236 g         2002 t
ORIGIN      
        1 ctgaagtgaa tattatacat ggaaatcgtt tcttgccata tttttcttca aatattgaaa
       61 atgcaccctg tagtatcttt tgtgcgtcta tacttcacaa aacaaacttt tattattata
      121 atataggtgt atgactaatg ggggggttgg aatgaaaaat attataagct ttgaatcatt
      181 agaccgggaa gaatatagtc atataccgct gttaaaaatg tcagataata atcatggcgg
      241 attgcctata ttcgtacgga aatatacctt gaactcctgc aataagggtc agctacacag
      301 acatgaattt atgcagataa actatgtttg ccagggaaaa ggaggccatt acattaacaa
      361 gcaggaattc aaaattatta aaggggatat atttgtgatt cctccatata tacctcatac
      421 tataaaaaca gatgaagatt caaatataca aatattcgag tttgagtttg tccctgaatt
      481 tgttaatcag gattttgaaa gtatagcaaa tattgagtca ttctttgatt ttgcttatat
      541 tgaacccttt ttagttagtg agagtcaggt taaaccacgt ttgaaccttg taggtaagat
      601 tcagaatgag gttgagaata ttcttaccga agtgttatcc gagtataatg aaaagaagcc
      661 tggatacctg ctcgttatta aatccctatt gttaaaactt ctggttatgg taggacgtga
      721 gtttacaagg gatctggaga attcagaatc ccgtacggca ttcgacagac atagagattc
      781 catttttgga gcaataaagt atattgatga acattttgct gaggacttaa atgtagaaga
      841 ggttgcaaaa aaattcatgc tatcccagtc atattttagt tatttattta aaagcataac
      901 atccaagact tttacagaat atgtaaacga tatacgtata tcaaaggcta tggagctttt
      961 gaaggactca gacaagaggg tgctggagat atgttatgaa gtaggtttta ataatgtcaa
     1021 ccattttaac cgcttgttca ggcagcagac aggtatatct ccgctggctt atcgcaagca
     1081 gaatccagaa aaacacaatt agtgctttaa tttcaataag cgctttaata tcaattagcg
     1141 ctatagttta aattagcgct ttaatatcaa ttagagctat agttcaatta gcgctttagt
     1201 tacaattggc tctttaatat tcaggcatat cttaattata acgagatgat agatatccac
     1261 cacctttgaa aaattttcta ctgatgcatt ataatagaat atgtcaattg aatagttaaa
     1321 tgtgcaaaga taatgtatat ttaatatttt ataatataat tgtaaagata ataatgattg
     1381 aagttataat acaaaatttt tgtaattaaa agcagtataa aaaagcaata taatgaaaaa
     1441 gtattttaag atgtattagt tggaatgtgt ttaaatatgt gagtaatcaa gattatatga
     1501 ttaaataata taggagggtt ttaattatgg cagaaaaact tgctttattt ggaggaccaa
     1561 agtcagtaca gtctgacgct ggcgatatgt ttgtatggcc gattattaca aaggaagatg
     1621 aagatgctgt attggatgta ctgcataaaa gagctatgtc cggtacaagt attacaaggc
     1681 agtttgaaaa ggaatttgct gcatggcagg gtacaagata tgctttaggt tttagcagtg
     1741 gtactgcagc tatacagaca gctatgtatg catgcaaggt gggaattggt gatgaaatta
     1801 tagctccaag cgtaacctat tgggcttcag ctactcaagc atattcactt ggtgcaacac
     1861 ctgtttttgc agaaatcgat acagatactc tttgtattga tccaaaggac atagaacatc
     1921 gtataaccga tagaacaaaa gcaattgttg tagttcatta tttgggtcat cctgcagata
     1981 tggatcctat tatggaaatt gcacgccgtc acaatataaa agtaattgaa gatgtttccc
     2041 atgctcaggg aggactgtat aaaggccgca aggttggtac aataggtgat gtaggtgcta
     2101 tgtctcttat gagcggaaaa tcccttgcaa ttggtgaggg aggaatgctc gtaactgatg
     2161 atgtagaaat ttatgaaaga tctcttgctt ttggacatta tgaaatgttc cgtgcagatg
     2221 aaattcagac agagtattta aaaccattgg cagggcttcc tttaggcggt tataagtatc
     2281 gtatgcatca aatgagttct gctgtaggcc gtgttcaact taaatattat gatcagcgct
     2341 gtgaagaaat ccgtaaaggt atgaattatt tctgggatct tctggaggga gtacctggat
     2401 taaaagcaca tcgtacatct gaggattctg gtagcaatat ggcaggctgg tatgcacctc
     2461 atggaatata taaagctgat gaacttgaag gattatcagt aacaagattt acagaagctg
     2521 taagggcaga aggtgtagaa tcatgttatc caggatgcaa cgctgcactt catacacatg
     2581 ctttatttaa tactgctgat gtatacggcc atggtaagcc aacaagaata gctaattcca
     2641 acaaggatgt aagagaattt gacaagagtc tgcctataag tgagaaaatt ggctccatga
     2701 tatatgctat accatggttt gtacgctaca gacctgaaat aattgaacag tatgcaaaag
     2761 cataccgtaa agcagcagaa aattataaag aattgctgaa ggatgatcca ggcaacccgc
     2821 caagattggg tggatggcac ttctttagac atcgttaatt aatagaatgt atttataatt
     2881 gaatagaaca atatgttata agcccctgtt gcaaaaaacg caggggttta ttttttctaa
     2941 taaatcgggt tatctacata agaatgggac ggaagaatgg gcggagctat cgagctaccc
     3001 ctgagaaaat atggagatta ctagagtgaa aatttaattt gctctcgggg tttcattgaa
     3061 agttttataa ttcccactga ataaagtaca gaatagagat tttagaacaa agggagatat
     3121 gctggatgtt tataggaagg gaacaggaac tcaatgatct agaaatgatg tatcaaaaag
     3181 ataattttta atgtgctatt atatatgata gaggacgggt tggaaaaatc agacttatac
     3241 aggagtttat taaaggggaa aaggcatttt ttttcagcga tagaatctaa catgtatagg
     3301 aatctggaac aatagagcaa ggtcatatac agtataacat taccgaggat gaagtcactt
     3361 ccagctttcg gtaagcaggt gttaggatat caaagccctt tatatggacg gcagaaaaaa
     3421 tagaaaaaag acattttaca aattggaaga tactatgttt gttttctggt atcgtttagt
     3481 gcttcctaac ctatttttaa tacaatcggg agagggagaa tacctttatg atagtatttt
     3541 gttgttctag atgagttata tcgggatata tagaatatat gtcatagggc ttttggcttt
     3601 ttcgatatat tgacatatat ataaaaccca tttataataa aagtgttgat aagccaagtg
     3661 acaattattc aaacttaatt gtgaaccatg tgggcaatta actcagctgg tagagtgtca
     3721 tcttgacgtg gtgaaagtcg ggggttcgag cccctcattg cccaccaaat gtaatgagcg
     3781 agattgatac aattaaggtt tgcctgcaac tactgaatct ccgtagcttg ggtcattgca
     3841 gaacctggag tttggatgcc tgcactggta tccatatcat ctatctctgt ctgctttacc
     3901 cactggctta aagtattctt gggtacacct aattctacag ctgcttttgt gttccctatc
     3961 tttctcataa gttttaccgc ttctgtcttg taatcttttg tatactgctt agctgttatt
     4021 ttacattatc tgacataaat aacctccgtt ttttcctgtt cctatactat ttgtccgagg
     4081 tctttgtgtc aagtattagt atagaaatta tgttaaataa aattaaacag actagtagtc
     4141 cacggcagaa aaattagatg caattatatc cctaatggaa gctacaggtc gtatgaaaga
     4201 ttttttatga tatatattat ttagcaacaa actttaattt tgatgggagg aagcttcaaa
     4261 gaatataata tttaaaaccc tgccatttca ataataagag aaaaataaaa aaattatgaa
     4321 attgggtata atactattat aaatattttg gattcgatag aggaaatact gaaagtatag
     4381 ggctaattaa tattccaaaa gataaggtta attttaatat tgcacattgt gttacattgt
     4441 ggtaccctat aaataggagg atggtgatgt agatatgagt actgttactg taagattgaa
     4501 cgaggaagaa gcaaaaatat ttaatgaata ttcaaaatta catggggttc cattgtcaac
     4561 tttattcaaa aaaacccttg aagaaaaaat ggaagatgaa cttgatatgc aggttataaa
     4621 agaatatgaa aaaagtcttg aaaatggcta tactgaaacc tttacccatg aagaagtcaa
     4681 aaaaatgtta ggtatgtaaa tatgaaatac caagttttat atacaaaaaa agcaataaaa
     4741 caattatcaa aaatagacgt aaatcaaagg catataattt tagcatggat agaaaagaat
     4801 ttggtaggaa tagataatcc tatgatttat ggaaaagcct taaaaggcaa tttgagagac
     4861 tactggagat atagaattgg ggattataga ataataacag aaataaatga tgatgaaatt
     4921 aaaataatag tagtgggaat aggtcataga aaagatattt atgaaaggct ttaaaacctg
     4981 ttaaagagtg aggaaactac agataataaa aaggaaaaga tataattaaa tgcccctgct
     5041 gcaacgatgg aaaaatgaaa acagtccgat gtggtatact caaagctaaa attgtatcaa
     5101 tctgacttac tatagacaca actattaccg gcgctaaggt ttttagacct ttaaattata
     5161 gaaaaagact agctccatta aagaggcagt ctttttctat attcccgtgg cggcagtccc
     5221 tttatttttt taaacatttt agagaaaagc agcggatctt cataacctac agacctagaa
     5281 atgtcgccga ttgtaaggtt gtaatttgcc attaaatcac aagccttgtt aatcctgtaa
     5341 tcgatcaaat aattctgagg actggtgtta aaaaattctt taaaaattga acccatataa
     5401 ctgcggtcca gaccaagata agctgctatt tctgaaacac ttatctttct ggaataatta
     5461 agttcaataa actcaatcag tttctttatg tatacctcct tcctgttaac agttacatta
     5521 tatgaagaag cttgttcatt ttcttcaata agcttggaaa gaaagctgta aaacaaacca
     5581 agcagcttca tctctcttcc gtttttaata ttttttgttt ctatcatttc tttaaagcag
     5641 gtttcaatat aatcatcttt agtataggta aaaatcgggt tatcccagct aagattagcc
     5701 tgcttaaggt aatcctctgc ttttagtcca tgaaacccta tccaggtgta atgccagggc
     5761 aagcctatat ccgcctggta aaaagttact attccggggc aaataaggaa tccttgtcct
     5821 ttacttagtt tgtatgtggt atttccaacc tggaaaacac catttccctc cgttacatag
     5881 tgaataagga aatgatctct tactgccggc ccataaaagt gtccgggttt gcatttctcg
     5941 gtcccgcatt gatacatatt taaatctgta aggtttagcc tgttaatagg cacaacttct
     6001 atcataacag gttatcacat cctgtctgta ttataaggta tactagcatt ataccatatt
     6061 agtatagcaa tgtaccattt actttgtagt ataaattgtt aaaatattag tatagtagtt
     6121 ctatgcaacg caatgatagc ccatgatgaa taattcttca tacattttga tgaatagtga
     6181 ggtgtaaaag tctatgaaaa aaattgcatt tattggagca ggcagctttg gctttacaag
     6241 gtctcttgta cgtgatattc tttcatttcc ggcattcgct gattgtactc tggctcttat
     6301 ggatattgat gatgaaagac tttcctacat taagaaagcc gt
//
LOCUS       DAOSDD010000243         6332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4441_length_6332_cov_4.618289, whole genome shotgun sequence.
ACCESSION   DAOSDD010000243 DAOSDD010000000
VERSION     DAOSDD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4441_length_6332_cov_4.618289"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK733_16370"
     CDS             <1..665
                     /locus_tag="PK733_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12148.1"
                     /translation="CIYKRPGTHSNPSTSGTTGTSGTTGILEGYRYIDKIVPIKKTIL
                     ANAILGFGVISVNMAFYPFAFDNLKVTEKGWGLMMSIFYSSNLISMAISLWLNKKISC
                     IKRKRVIYDAIIYISLITISCIWFVYSRTYSLPLILIVQLLEGALISLCAILLMSKLQ
                     SVTEEGILGRVTGINDFITSISKIAGIFLAYNIIKRRSPIYVFLFNSTCFLIFALYNL
                     VL"
     gene            complement(698..1072)
                     /locus_tag="PK733_16375"
     CDS             complement(698..1072)
                     /locus_tag="PK733_16375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ12149.1"
                     /translation="MVAGYVNKPGYLNEAQIKEMKDIISFQSHTLSHPELDKLNEEEL
                     ENEFVESKAIIEEITGEPVYTIAYPYGCYNQKVADIAAKHYSYAVNTKKGVYDGLAGN
                     YEINRICICRSNQFGDFMRLIH"
     gene            complement(1102..1422)
                     /locus_tag="PK733_16380"
     CDS             complement(1102..1422)
                     /locus_tag="PK733_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12150.1"
                     /translation="MHTGLNIKLKIIVIIMFLSPIFIMKGTGARKGVIILAYHAVNNN
                     IKGIEELFVKPEEFYKQIEYMSKRKYTPLHFNEVSSYWKYKKPVIITFDDGYVDNYHI
                     AILY"
     gene            1755..3218
                     /locus_tag="PK733_16385"
     CDS             1755..3218
                     /locus_tag="PK733_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ12151.1"
                     /translation="MYKLDQNSTPLFDALKKYVDDNVIPFHVPGHKKGRGIPELKDYI
                     GDLALNMDVNGMEDLDFINSPTGVIYESEALMAEAYKAENAFFLVNGSSSGVQTMILS
                     TCEPGSKIIIPRNAHKSTIGGVILSGAIPVYVQPEISRNIGIAMGTPLSSIKNAVRKE
                     PHAKAVFIINPTYYGVTSDIKAIINIAHTHGMAVLADEAHGAHMPFHKDFPVTAMETG
                     ADISTVSIHKTAGSLTQSSALLLKSKIVSSEKVKEVLNLTYTSSASYLLMCSLDIARK
                     QMAVKGAEILEEVLGLARWARQEINKIDGLYAFGKEIIGSPGCCNFDETKIGINVRNL
                     GYTGYQIESMLRKEYNIQIEMSDLYNILALITIGDRKEDVASLINALKDISSKTEIRE
                     LKNCLILPPVPEMIVSPRDAFYNSKKIVKLENAEGEIAGEMIMAYPPGIPVICIGERI
                     TDDIIDYIRILKGEKCKLQGTCDPHVNFIRVLGKESN"
     gene            3221..3445
                     /locus_tag="PK733_16390"
     CDS             3221..3445
                     /locus_tag="PK733_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12152.1"
                     /translation="MPVCRSWGSHREHELRGTVLSIMEYGGLSPFNMENCPPYFALDY
                     AGLSPVFALDYAGLSPVFALDYAGLSPISF"
     gene            complement(3446..4717)
                     /locus_tag="PK733_16395"
     CDS             complement(3446..4717)
                     /locus_tag="PK733_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOJ12153.1"
                     /translation="MEILSLGSTGPDVKLVQSLLNRIGYNAGAVDGIFGSITRQAVIQ
                     FQMNNGLTADGVVGPQTWAVFERLLRGYDIYTIRPGDTFYKIARRYYTTVNAILTANP
                     GINPNMLIPGQRIVVPYGIDIVFDDIDYTYEIIERQIQGLKVRYPFLETGTIGRSVMG
                     KNLYYIRLGRGGRQVSYNGAHHANEWITTPLLMKFIENYSKAYSTGGSIRGYNIPQLY
                     NTSSIYIVPMVNPDGVDLVNYWPNYTDPAYRQAALLNKTGLPLPWVWKANIRGVDLNV
                     NYPASWEKEKQNQIEQGITGPAPRDYGGEEPLSEPETIAMANFTRNHNFNMVIAYHTQ
                     GEVIYWQFENLAPPESITIGNIFSEISGYALVGGPGEAAYAGYKDWFIQEYGRPGFTI
                     EVGRGTNPIPISQFPVIYRQNEEILLLSPLL"
     gene            complement(5217..6176)
                     /locus_tag="PK733_16400"
     CDS             complement(5217..6176)
                     /locus_tag="PK733_16400"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ12154.1"
                     /translation="MLNKMLYNKLKITFVLLLFSICLLFVTVITGNSAVYAVEKATAI
                     TQYTVQKGDTLYIIATKYGVALSKLKSENSLISDIIYPGQVLKIPANTRMDGNVYAVA
                     RGDSLYFIARKYGVTINEIKSTNNLTGDLIFPGQVLVIPGRSLAAIIADKGINKSSGV
                     FEILVDKSDHVLSVYANGSILKSYHVELGDGGSADKEIAGDHRTPEGTFYICEKLVLN
                     PADMYLGSRWMRLSYPNIEDAARGLGKEIIDQQTYNSILTAYNKGKIPPQRTALGGGV
                     GIHGGSIPEFGKDWTWGCVGLRNKDVEELYEYIRVGTKVIIQK"
BASE COUNT         1948 a         1218 c         1080 g         2086 t
ORIGIN      
        1 aatgtatcta taaaaggccc ggtactcata gtaatcctag tacttctggt acaactggta
       61 cttctggtac aactggtatt ctggagggat atcgctacat agataaaata gtcccaataa
      121 agaaaacaat tctagcaaat gctatattag gctttggagt catatcagta aatatggctt
      181 tctacccctt tgcttttgac aacctgaaag ttacagaaaa aggttggggt cttatgatgt
      241 caatatttta tagttctaat cttatatcta tggcaatatc actatggctt aacaaaaaaa
      301 tcagctgtat aaagaggaaa agagtcattt atgatgcaat catatatatt tcccttataa
      361 ctatatcatg catatggttt gtctatagcc gaacatatag tcttccatta atattaattg
      421 ttcaattact ggaaggtgcg ctaatatccc tatgcgctat tctgcttatg tcaaagcttc
      481 agtcggttac tgaagaaggc atactcggaa gagtaaccgg aataaatgat tttatcacct
      541 ctattagcaa aattgccggt atattcctag cttataatat cattaaaaga cgttctccaa
      601 tatatgtctt tctatttaac tctacttgtt tcttaatatt tgccttatac aatcttgttt
      661 tatagacttt tccttatata atattgtttt tcctttttca atgtatcaga cgcatgaaat
      721 caccaaattg attggaccgg catatacata ttctgtttat ctcataattt ccagccaacc
      781 catcgtaaac accttttttt gtattgacag catagctata atgttttgct gcaatatccg
      841 ctactttctg attatagcaa ccatatggat aggcaatggt atatacaggt tctcctgtta
      901 tttcttctat aatcgccttt gattccacaa attcattttc aagttcttct tcatttaatt
      961 tatcaagttc aggatgactt aacgtatgac tttgaaatga tataatatct ttcatttctt
     1021 ttatctgagc ttcattcaaa tagcccggtt tatttacata tcctgccacc ataaatacag
     1081 ttgccttaaa gttaaacttt tttagtatag gatggctata tggtagttgt ctacatagcc
     1141 gtcatcaaat gtaattataa ccggtttttt atatttccag taactgctta cctcattaaa
     1201 atgcagaggt gtatatttcc tttttgacat gtattctatt tgtttgtaga attcttcagg
     1261 ttttacaaaa agttcctcta tgcctttaat attattattt acagcatgat atgcaagtat
     1321 aataacacct ttccttgcac ccgtaccctt cataataaaa attggagaaa gaaacattat
     1381 tattactata atcttaagtt ttatgtttaa accagtgtgc accaaatcac catcttttta
     1441 aatagtatat acacaattat ttatttaaat tcatactata aagcatgaat ctaattgtta
     1501 tatgttttgt tttatacagt tttgttttat acagtttact ttatacatac agctttacta
     1561 tatgtggctt cattttatgc agcttcattt ttatacaact ttattttata caactttttg
     1621 ctttatataa ctttttgcag aaaattgcat agcaaactcg cttctttcag gattcagggg
     1681 gagcttgcaa tctctcactg aataaagttg taattataat ttggctaaac aatatattaa
     1741 ggaggattcc cgatttgtat aaacttgatc aaaatagtac tccattgttt gacgctctta
     1801 aaaaatatgt agatgataat gtaatcccat ttcatgtgcc tggtcataaa aaaggtcgcg
     1861 gcatacctga gttaaaagat tatataggtg atttagcttt gaatatggat gtaaacggga
     1921 tggaggatct ggactttata aatagcccca ctggggttat atatgaatcc gaagctctta
     1981 tggctgaagc atataaggca gaaaatgcat tttttcttgt aaatggttca agttccggtg
     2041 ttcagactat gatcctgagt acatgtgaac ccggaagtaa aattattatc cccagaaatg
     2101 ctcataaatc aactataggt ggagtaattc tcagcggtgc aataccagta tatgtacaac
     2161 ctgaaattag ccgaaacatt ggtattgcaa tgggtacacc ccttagcagt ataaaaaatg
     2221 cagtcaggaa agaacctcat gcaaaagcag tattcataat taatcctaca tattatggag
     2281 taacttccga tattaaagct atcataaata tagctcacac acatggtatg gctgtacttg
     2341 cggatgaagc ccatggtgcc catatgcctt ttcataagga ttttcctgta acagctatgg
     2401 aaacaggagc agatataagt acagtaagta ttcataaaac agccggctca ttgacacaga
     2461 gctcggcgct gcttctaaaa agcaaaattg tttcatctga aaaagtgaaa gaggtattga
     2521 atctaacata tacttccagt gcgtcttatc tgcttatgtg ttcactggat atagcaagaa
     2581 agcaaatggc tgttaaagga gccgaaattc ttgaagaagt tcttggttta gccaggtggg
     2641 caaggcagga aattaacaaa attgatgggc tttatgcatt tggcaaagaa attataggtt
     2701 cacccggctg ctgcaacttt gatgaaacta aaattggtat taatgtaaga aatctcggat
     2761 atacaggtta tcagatagaa tcaatgctta gaaaagaata taacatacag attgaaatgt
     2821 ctgatctgta caatatcctt gcattaatta ccatagggga tagaaaagag gatgtggcaa
     2881 gcttaattaa tgcccttaag gatatttctt ccaagactga aattagagag ttaaagaact
     2941 gcctaatatt acctcctgtt cctgaaatga ttgtatcacc acgagatgca ttttacaatt
     3001 cgaagaaaat agtaaaacta gaaaatgcag aaggagaaat tgccggtgaa atgattatgg
     3061 cataccctcc tggaatacct gttatctgta ttggtgaaag aataacagac gatattattg
     3121 actacatcag gatactaaaa ggtgaaaagt gcaaactgca gggtacctgt gacccacatg
     3181 taaactttat aagggtattg ggaaaggagt caaattagct atgccagtct gccggtcatg
     3241 gggcagccac cgagaacatg aattaagggg gacagttctc tcgattatgg agtatggagg
     3301 actgtccccc tttaatatgg agaactgtcc cccatatttt gctctcgatt atgcaggact
     3361 gtcccccgta tttgctctcg attatgcagg actgtccccc gtatttgctc tcgattatgc
     3421 aggactgtcc cccatatcat tttagctaca ataatggact caagagtaag atttcttcat
     3481 tttgcctata tataactgga aactgggaaa ttggtattgg gttcgtaccc ctgccaactt
     3541 caattgtaaa acccggtcgt ccatactcct gtatgaacca atccttgtac ccggcataag
     3601 cagcttctcc tggacctcct acaagagcat atccgctaat ttcggaaaat atattaccaa
     3661 tagttataga ttctggtgga gcaaggtttt caaactgcca atatatcact tcaccttgtg
     3721 tatggtaagc tattaccatg ttaaaattat ggtttctggt aaaattggcc atagcgattg
     3781 tttcaggctc tgatagaggt tcttctcctc cataatctct cggagctgga cctgttattc
     3841 cctgctcaat ttgattttgc ttctcttttt cccagctggc aggatagttt acatttaagt
     3901 ctacgcccct gatattggcc ttccaaaccc atggtaaagg caagcctgtt ttattaagga
     3961 gcgccgcctg tctgtacgca ggatcagtat aattgggcca gtagttcaca agatccactc
     4021 catcaggatt gaccattgga actatatata tactgcttgt attatataat tgaggtatat
     4081 tatatcctct tatactccct ccggtagaat atgctttact gtaattttct atgaatttca
     4141 ttaataaagg agtagttatc cattcatttg catgatgagc cccattgtag gaaacttgtc
     4201 ttccgcctct ccccagtctt atataatata ggttttttcc cattacactt ctgcctatag
     4261 tgcctgtttc aagaaatgga tacctcactt tcaagccctg tatttggcgt tcaataattt
     4321 catatgtgta atctatatca tcaaatacta tatctatccc atatggaaca acaattcttt
     4381 gtccaggtat aagcatattt ggattaatac cagggttcgc agttaatata gcatttacag
     4441 tagtataata tcttcttgct attttataaa aagtatcacc gggacgaatt gtatagatat
     4501 catatcctct aaggagtctt tcaaaaacag cccaggtttg ggggcctaca acaccatcag
     4561 ctgtaagccc attattcatt tgaaattgaa ttaccgcctg ccgggtaatt gaaccaaata
     4621 tgccatctac agcacctgca ttataaccta tcctgttgag aaggctttgc acaagcttta
     4681 catcaggccc ggttgagcct aaacttaaaa tttctatttt aactcaccac ttttcctttt
     4741 ttctataaaa acttcgttct aaaatcttca taatagacta atactacgtc aatttgggaa
     4801 gcgatgtctt ccgggataat ttttgatagc atcgggaaga tgtcgcacag ggaaatgtct
     4861 ctaagaaagc cttgcgaccc ttccctcaga tgcattcctt acgggtcagc aaaaattacc
     4921 ctccagaaat gcgctttcca ttggcagcat gacaaagtag tattactaat agattttcta
     4981 aaacctatat aataacggtc tacataatca taatatgaaa atttcaagaa aatgttcatg
     5041 gtaggttatg aaagcgactt gagcttccgg agagcttttt cgaggtatgc aggcatatct
     5101 ccaagccagt ttaaaaagct gtgataggct cggaatgtcc caggtgtctt gagagcgaat
     5161 accgtgtttc atggaagaac tctgcaattt ttgcaggatt ctcccatgaa ataaacttat
     5221 ttttgaataa taactttagt acctacacgg atatattcat ataattcttc cacatccttg
     5281 tttctaaggc ctacacaccc ccatgtccaa tcctttccaa attcgggaat gctgccaccg
     5341 tgaatgccta ctccgcctcc aagagcagta cgctgcggag gaattttacc tttattatat
     5401 gctgtcaaaa tagaattata tgtctgctga tcaattatct ccttgccaag tccacgagca
     5461 gcatcttcaa tatttggata gctaagcctc atccacctgg atccaaggta catatctgca
     5521 ggattcaaaa ccagcttttc acatatatag aatgttcctt caggggtcct atgatcgcct
     5581 gcaatttcct tatctgcaga gccgccatca cccagctcca catggtatga ttttaatatg
     5641 cttccatttg cataaaccga aagaacatga tcggatttat ctacaaggat ttcgaaaact
     5701 cctgaagatt tgttaatgcc tttatcggca attatggcag ccaatgacct gcctggaatt
     5761 accagcacct gtcctgggaa aatcaaatcg ccagtaagat tattggtgct ttttatctca
     5821 tttatagtaa ctccatattt tcgtgcaatg aaatatagac tgtcccctct tgcgacagca
     5881 tatacattac catccattct tgtattggca ggtattttca gcacctgtcc cggatatatt
     5941 atatctgaaa taagcgaatt ttcacttttt aacttactca aagcaacacc atatttggtg
     6001 gcaataatat aaagtgtatc tcctttttga acagtatact gagttatagc tgttgccttt
     6061 tcaactgcgt aaactgcact atttcccgtt attactgtta caaataacaa gcaaatagaa
     6121 aacagtaata aaacaaaagt aatctttaac ttattataca gcattttatt tagcattaca
     6181 ttttccccct ttactaagaa aatattgtta accaacaaaa aaatgttgcc ggaatattgt
     6241 aacatgaatg cagcattaaa ttctatagta aattaaggtg caaatgtatc gaaatgcatc
     6301 tatttgtata tgttgtataa ttgattacac tg
//
LOCUS       DAOSDD010000244         6211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4537_length_6211_cov_4.334470, whole genome shotgun sequence.
ACCESSION   DAOSDD010000244 DAOSDD010000000
VERSION     DAOSDD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_6211_cov_4.334470"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PK733_16405"
     CDS             <1..825
                     /locus_tag="PK733_16405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ12155.1"
                     /translation="TQVVQIMFVTIFLMITGIIDDRFDLSSKLKLAIHSLASLITVLM
                     GIRFNISGLPIPDIIVTVIWISAIINAVNLIDGLDGLAAGITTISCAGFCTIGLIYNS
                     SIIVFLSAAIVGSCLGFLKYNFRPAKIFMGDTGSIPLGYNLAVIGILCQNLIVNVTSS
                     TKPMTVISGSTVLIIPAIILGVPIYDMTLSIFRRVVNRKPIFKPDRSHFYNLMMELKG
                     LSHKKTVLAIYLVSFFLSCISILLVFLNSVLRVAVICVLTIAAAFLSYLLNFIRVD"
     gene            1002..1853
                     /locus_tag="PK733_16410"
     CDS             1002..1853
                     /locus_tag="PK733_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12156.1"
                     /translation="MVYIEPELIEWVKYEGALIPDVPPHIEVKMSNEYARYLFKTYKP
                     YFIRWVSDFDCSHETEWWYVIKEKEINNNELEYSKYFRRYLKCGLKTCVVKKIDAEYI
                     AYNGYKVYKNAFKRYDTFEKPIDEKDYMRHMLSFVNNSNYEFWGVFHKKSNALIAYSQ
                     NLLRNDCCEYSSMKFDPEFLDLRPSYPLIYETIKYYINDKKLIYVSFGARSIYHKTAI
                     QEFLVTRCKFRKAYCRLNIIYSFKIKLIVNILYPFKNIISSINTGITNKIAALLQQEY
                     VRRSFLN"
     gene            1884..3044
                     /locus_tag="PK733_16415"
     CDS             1884..3044
                     /locus_tag="PK733_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOJ12157.1"
                     /translation="MEGIDATTNPAKSINSQLEKSININEQIIKNRIKRVNIDELLTR
                     KPVIFDINKLSSYLENQVVVVTGGGGSIGSELCRQVASYNLRRLIIIDIYENNAYYIQ
                     DELAYKYPYLDLEIVIANVRDKERIDYIFDKLCPDVVFHAAAHKHVPLMEKNPGEAIK
                     NNVFGTLNLVECSHKYNVKKFILVSTDKAVNPTSIMGATKKIAEMIVQVYDTQSETKF
                     SAVRFGNVLGSSGSVVPLFQMQIERGGPVTVTHPDITRYFMTVGEAVQLVIQSGDMSC
                     GGEIFVLDMGEPISIYKLARDLIERSGFIPDIDIKIIFTGLRPGEKLYEEPLLTDDGL
                     QLTQNSKILITRPDFPDVDTLKTQIEMLDTININDTAQVKAYIKKVVPTYFI"
     gene            3112..4977
                     /locus_tag="PK733_16420"
     CDS             3112..4977
                     /locus_tag="PK733_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12158.1"
                     /translation="MQECSNDIRRILSLVMAFDRDGYNVKKIITAFDKTGIMAEHKTA
                     GNYDTGQKKKAYYLEGSSYQMGYLMGLLAEEEISRMTTDFTTNIISEFIGVKKQKKRR
                     LLEEVLISILYGFFKSYYSNLPIEIQDEIQGIYDGCKKSNPGTKVDIKHLFVLNAGID
                     VLCSVVYTGKLAAREIPGLKPSDFNLPIMCNAFSIFGRPAGNGHYLGRDFMFATGGVF
                     QDTAAHIIYNPEKHRYEKGANYYINNDYHANNNCCAKTGCNVNNSCHTRTNSYTNNYA
                     NNYANNYANNYANNYANDYDALPFVSVSTPGMVGSISAMNINGVGFGVDMSPAANCNP
                     SKIGTNSILLTRLCAQYGYDANKAVEIMKNTSRGVSWNYIIADGINDRACIVEAGYSE
                     AKPDFTKIPPSKYTKRLPDLEFIDSHKSAKFEKGLMVRWNDYKYPEEYLSFNERLWKS
                     YRERCFSKKKLYNNAFEEKGYINTSHRDKNCPSTFYFAPQREIHDKIVITTNHYIIPE
                     MRFYGMNQWTSRVVGNKINDIQWRYDELNNQILNTLEENGLIDYSTAKKLIDFLAPYG
                     KYPAYYENNARSKDGKQIRIEGCTSIFDLKKRKVESHFGYYCDEWVKTTLPLYFY"
     gene            5162..>6211
                     /locus_tag="PK733_16425"
     CDS             5162..>6211
                     /locus_tag="PK733_16425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOJ12159.1"
                     /translation="MLFNSLEFLIFYPIITILYFILPYRARWVLLLAGSYYFYMAWRP
                     EYIILIMISTFVSYFTGIKIGQANNKSTKKKYLNLSLLINLGLLIVFKYFNFFNDSLR
                     TLFTQLNLPYNVPGFNLLLPMGISFYTFQTLSYSIDVYRGTMKPETHFGIFALYVSFF
                     PQLVARPIERADRLLPQFYNKNKFDYDRVTSGLKIMAWGFFKKVVVADRLGIAVSAIY
                     NNPVKHNSIQFIIATLFFTFQIFCDFSGYSDIAIGSARIMGFELMENFKRPYFSKSVA
                     EFWRRWHISLSSWFKDYLYIPLGGNRVSKARNYFNLFITFLASGLWHGANWTFVVWGA
                     LHGAYIVLGRMLAPVR"
BASE COUNT         2124 a          951 c         1199 g         1937 t
ORIGIN      
        1 acacaggtag ttcaaataat gtttgttaca atattcttga tgataacagg cattattgat
       61 gatagatttg atttaagttc aaaattgaaa ctggcgatac attctcttgc ttctttaatt
      121 actgttttaa tgggaatcag attcaacata tccggcttgc cgattcctga tataatagta
      181 actgtaatat ggatttctgc aataataaat gctgttaatc tcatagatgg gcttgatggc
      241 cttgctgcgg gaataacaac aatttcctgt gcaggctttt gtacaattgg actcatatat
      301 aattcaagta ttattgtatt tctgtcagct gctatcgtgg gctcgtgcct ggggtttctc
      361 aaatacaatt tcagacccgc caaaatattt atgggtgata ccggaagtat tccacttggc
      421 tataatcttg ctgtcatagg aatactgtgt cagaatctta ttgtaaatgt aacttcatca
      481 acaaaaccaa tgacagtaat ttcaggtagt acagtgctaa taataccagc catcatactt
      541 ggagttccca tatatgacat gaccttgagc atctttagaa gggttgtgaa taggaaacct
      601 atttttaaac cggatagaag ccatttttac aatcttatga tggagttgaa agggctgagt
      661 cataaaaaaa cggtccttgc catatactta gtttcttttt ttctttcctg catttctata
      721 ttattggtct ttttaaatag tgtattaaga gttgcagtta tttgtgtatt aacaatagca
      781 gctgcatttc taagctatct tttgaatttt atacgtgtag attgaaatat aaagctaaat
      841 tgaacaaata tttgaaacat tttataagtg aaatgaatat tatgctactg ataagaatta
      901 ttatgtcaac ataaacagta gcataattgt aatatacctg tattaaccat aacacatagg
      961 aagatatcag caaatactaa taaaatgatg gagttgcaaa gatggtttat atagaacctg
     1021 aacttattga atgggtaaaa tatgagggtg cattaatacc tgatgtgcct ccccatattg
     1081 aagtgaaaat gtcaaatgag tacgcaaggt acttatttaa aacatataaa ccttatttta
     1141 taaggtgggt aagtgatttt gactgcagtc acgaaacaga atggtggtat gtaatcaaag
     1201 aaaaagagat aaataacaat gagctggaat atagcaaata ttttagacgc tatctaaaat
     1261 gcggtttaaa aacttgtgtt gtaaagaaga tcgatgcaga gtatattgca tataatggat
     1321 acaaagtcta taagaacgct tttaaaaggt atgatacttt cgaaaaacca attgatgaaa
     1381 aagactatat gaggcacatg ctttcttttg taaataactc caattacgaa ttctggggtg
     1441 tatttcataa gaagtctaat gctttaattg catatagtca aaacctttta agaaatgact
     1501 gttgtgaata ttcatctatg aagtttgacc ctgagttttt agatttaaga ccaagttatc
     1561 cattaatcta tgaaaccatc aaatattata taaatgataa aaaattaata tatgtaagtt
     1621 tcggggccag aagtatatat cataaaactg ctatacagga atttttggtc actagatgca
     1681 agtttagaaa agcctattgc agacttaata ttatatatag ctttaaaatt aaactaattg
     1741 ttaatatact ttatccattt aaaaatatta tttcaagtat taatacaggt attacaaaca
     1801 aaatagctgc gcttcttcag caagaatacg taagaagaag ctttttaaat taaaaaatag
     1861 cgacaatccg gagggacatg attatggaag gaatagatgc aacaacaaac cctgcaaaat
     1921 ctattaatag ccaattagaa aagtctatta atattaatga gcaaataatt aaaaacagaa
     1981 taaaaagagt aaatattgat gagcttttaa caagaaaacc tgttatattt gatataaata
     2041 aactatcatc ataccttgag aatcaggttg tggttgtaac ggggggtggt ggatcaattg
     2101 gctccgaact atgcagacaa gtggcatctt ataaccttcg ccgcttgatt ataatagaca
     2161 tttatgaaaa taatgcttat tatatccagg atgaattggc ttataaatat ccttaccttg
     2221 atcttgaaat agtaattgca aatgtaaggg ataaagaaag aatagattat atttttgaca
     2281 aattatgtcc tgatgtagtt tttcatgcag cagcacacaa gcatgtgccc ttaatggaaa
     2341 aaaaccctgg agaagcaata aagaacaatg tttttggcac tctaaatctc gtagagtgtt
     2401 cacacaaata taatgtcaaa aagtttatcc ttgtttctac cgataaagca gtaaatccca
     2461 ccagtattat gggcgctact aaaaaaattg cagaaatgat agtacaggta tatgatactc
     2521 aaagtgaaac aaagttttca gcggttagat ttggcaatgt actcggaagc agcggaagtg
     2581 ttgtaccact ttttcagatg cagatagaac ggggagggcc tgttactgtt acacaccctg
     2641 atattacaag atattttatg actgtaggtg aagccgttca gttagtaata cagtcaggag
     2701 atatgtcctg cggcggcgaa atatttgtgc tggatatggg tgaacctatt agcatttaca
     2761 agttggcaag agaccttata gaacgttcag gttttattcc tgatattgat ataaaaatta
     2821 tatttactgg gttgagacct ggtgagaaac tatatgaaga acctctgctt accgatgatg
     2881 gattacagtt gacacaaaac agcaaaatat taataacaag gcctgatttt ccggatgtgg
     2941 atactttaaa aactcaaatc gaaatgctgg atacaattaa tataaatgac actgcccaag
     3001 taaaagctta cattaaaaaa gttgttccta cttactttat ataatgtatt tttttttggg
     3061 tgttatctat agtataaatg aagtataagt gtgaaatagg aagggatcaa aatgcaggaa
     3121 tgtagtaacg atattaggcg tattttgagt ttagtaatgg catttgacag ggatggctat
     3181 aatgtaaaaa aaataataac tgcatttgat aaaacaggaa ttatggcgga acacaaaact
     3241 gccggaaatt atgatacagg acagaaaaag aaggcatatt atctggaagg cagcagttac
     3301 cagatgggat atttgatggg acttcttgca gaagaggaaa tatcacggat gacaactgat
     3361 tttacaacta atattatatc ggagtttata ggtgttaaaa agcaaaaaaa acgcaggctt
     3421 cttgaagaag tcctcatatc cattttgtat ggttttttca aaagctacta ttcaaacctt
     3481 ccaatagaaa tacaagatga aatacagggg atttatgatg ggtgcaaaaa aagcaacccc
     3541 ggaactaaag tagatataaa gcaccttttt gttctaaatg caggtattga tgttctttgc
     3601 tcggtggtat acacgggaaa attggctgca agggaaatac cgggtttaaa accctccgat
     3661 tttaatctgc ctattatgtg taacgccttt tcaatattcg gaaggcccgc aggaaacggc
     3721 cattacctgg gaagagattt tatgtttgcc acagggggtg tttttcaaga tacagctgcc
     3781 catataatat ataaccccga gaaacacagg tatgaaaaag gagctaatta ctatattaat
     3841 aatgactacc atgccaataa taattgttgt gccaaaactg gttgtaatgt taataatagc
     3901 tgccatacta gaactaatag ctatactaac aactatgcca acaactatgc caacaactat
     3961 gccaacaact atgccaataa ctatgccaat gactatgatg ccctgccttt tgtaagcgtt
     4021 tcaacgcctg gcatggttgg cagcatatct gctatgaata taaatggagt gggattcggg
     4081 gtggatatgt cgccggcagc caactgcaat ccctcaaaaa taggaaccaa ttccatcctt
     4141 ctgacacgtt tgtgtgctca atatggctat gatgctaata aagcagtaga gattatgaaa
     4201 aatacttcca gaggggtatc ctggaattac ataattgctg atggtattaa cgacagggca
     4261 tgtattgttg aagcgggata ttcggaagca aagcctgatt ttacaaaaat tcccccttca
     4321 aagtatacaa aacggctgcc tgatttggag tttatagata gtcataaatc tgcaaaattc
     4381 gaaaaggggt taatggtacg atggaatgat tataagtacc ctgaagagta tctaagtttt
     4441 aatgagagac tgtggaaaag ctacagagaa aggtgctttt ctaagaaaaa gctctataat
     4501 aacgcctttg aagaaaaagg atatattaat acaagtcata gggataagaa ttgtccatct
     4561 actttttact ttgcacctca aagggagatt cacgataaga ttgtaataac aaccaatcat
     4621 tatatcatcc ctgaaatgcg gttttatgga atgaatcaat ggacatcaag ggtggtagga
     4681 aacaaaataa atgacataca atggaggtac gatgaactta acaaccagat actcaatacc
     4741 ctggaggaaa atggattaat tgattattca actgccaaaa agctgattga ctttctggca
     4801 ccctacggca aatatcccgc ctattatgaa aacaatgcaa ggagcaagga tggcaaacag
     4861 ataaggattg aaggctgtac atctattttt gaccttaaaa aaaggaaagt tgaaagccat
     4921 tttggatact actgcgatga gtgggtaaaa accaccttac cactgtactt ttattagggg
     4981 atattagggg acatttctct ataattacta taattacgga aggtattgca ttttgcggct
     5041 aaaatactct ataatcaaaa tgggacattc cttgatatga atcaaaagga gacattcctt
     5101 gatatggacg ttgaagtgga cgttgaaggt atgccgataa ccttgaagag ggggctgtaa
     5161 tgtgcttttt aactcacttg agtttttaat attctatccg ataataacca tactatattt
     5221 tatactccca taccgtgcaa ggtgggtgct tttgcttgct ggaagctatt atttttatat
     5281 ggcctggaga ccggagtata ttattcttat tatgatatct acttttgttt cctattttac
     5341 tggtattaaa attggacagg ctaataataa gagtacaaag aaaaaatact tgaacctaag
     5401 tcttttaatt aatctgggat tgttaattgt atttaagtat ttcaacttct ttaatgattc
     5461 cttaaggaca ttatttacac agcttaatct tccttataat gttcccgggt ttaatctgct
     5521 tctgcctatg ggtatatctt tttatacctt ccagacattg agctattcta tagatgttta
     5581 cagaggaact atgaaaccgg aaacacattt cggaatattt gccctgtatg tatccttctt
     5641 tcctcagctt gttgcaagac ctattgagag ggccgacaga ctgctgcccc agttttacaa
     5701 caaaaacaaa tttgattatg acagagttac cagcggactt aagattatgg cctggggatt
     5761 tttcaagaag gtggtagttg ctgacaggtt gggtatagct gtaagcgcaa tatataataa
     5821 tcctgtaaaa cacaatagta tacaatttat cattgctact ttatttttta catttcagat
     5881 tttttgtgat ttttccggat attctgatat agcaatcgga agtgccagga taatgggttt
     5941 tgaattaatg gaaaacttta agaggcccta tttttcaaaa tctgtagcag aattttggcg
     6001 aagatggcat atatcattat caagctggtt taaagattac ctttatattc ctttgggagg
     6061 aaatagggta agcaaggcaa gaaattattt taatttattt ataacatttt tagcaagcgg
     6121 tctatggcat ggggcaaact ggacttttgt agtgtgggga gcacttcacg gagcttatat
     6181 agttcttggc aggatgctgg cccctgtcag g
//
LOCUS       DAOSDD010000245         6180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4572_length_6180_cov_5.157388, whole genome shotgun sequence.
ACCESSION   DAOSDD010000245 DAOSDD010000000
VERSION     DAOSDD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4572_length_6180_cov_5.157388"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1041)
                     /locus_tag="PK733_16430"
     CDS             complement(142..1041)
                     /locus_tag="PK733_16430"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ12160.1"
                     /translation="MVAVFERDLDPERIIRELAVKSGQSIFKGDTLIIFDEIQASERA
                     LLSLKYFYEKAPEYHVIAAGSLLGVALNREKYSFPVGKVEMMTLYPLDFEEFLWATGN
                     EALSVMIKEAYERMIPLSLHETALELYKTYIVVGGMPRAVIEYVTTKDFNFVLASQKT
                     LNDAYIADMAKYASPGETTKIMAAWNSVPAQLAKENRKFQYKLIKTGARAYEYETPLD
                     WLRTAGIINKCIRITEGKIPLSAYTDNSSFKAYMMDTGLLCSKFDIPANVIINTPHGF
                     NDFKGSLAENYVMQVLVVTSSPQ"
     gene            complement(1052..1174)
                     /locus_tag="PK733_16435"
                     /pseudo
     CDS             complement(1052..1174)
                     /locus_tag="PK733_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024550.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
     gene            complement(1423..2241)
                     /locus_tag="PK733_16440"
     CDS             complement(1423..2241)
                     /locus_tag="PK733_16440"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOJ12161.1"
                     /translation="MKIFIMTDMEGVCGVINHDDWVMPDGRYYEEGKRLLTMEINAAV
                     EGFFTAGATEIFVADGHGAGGINQSLLDKRTMFIRGFPKPYPFNLDETFDAVAWVGQH
                     AKAGTPFAHIAHTGWFNVLDYKINGISVGEFGQMAMCAASLGVRSIFGSGDEAFTKEA
                     SGLINGIETVAVKKGLNPGSGDKCDGEAYRNRNLAAIHYHPQRACELIREGAERALNR
                     FIANKESFQLLDLEPPFIRKVKYRLYKGTPAYTAYAKHESDFIEMMNSKEHTVI"
     gene            complement(2279..3304)
                     /locus_tag="PK733_16445"
     CDS             complement(2279..3304)
                     /locus_tag="PK733_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ12162.1"
                     /translation="MMGKIICFGEIMARLNPEGYLRLGQAKKLEISYGGAEANVAVSL
                     ANYGLNSAFVTKLPENDLSKAAVKDLRGYNVDVSDIVYGGERLGIYFAEKGASQRASK
                     VIYDRKYSSIATAGRQDFDWNKIFDGASWFHFTGITPALSDGTAEICLDACKWAKEKG
                     ITVSCDLNYRKNLWSSEKAGKVMSELIKYVDVCISNEEDAEKVFGIKADHTDVDGGQL
                     DHKRYRQVAQEISSRFGCKKVAITLRGSITANDNNWAGMLYTDGECCFSRNYLIHIVD
                     RVGGGDSFGGALIYSLVSGYSSQDAIEFAVAASCLKHTIEFDYNQVTADEVKSLIKSG
                     GTGRIQR"
     gene            complement(3566..4576)
                     /locus_tag="PK733_16450"
     CDS             complement(3566..4576)
                     /locus_tag="PK733_16450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12163.1"
                     /translation="MKICIIGSTGHYGIALDGIKNKKEISIAGIAPGSLGENVSSLYE
                     RLSKEYPSVQYFDNYCNMLDEIKPEIAVVCCYFADQTKVAGDALKRGIHVFMDKPVAT
                     SLEDLETLKNIYKQSGAHLSAMLAIRATPWFLTAHKSIKEGSIGEIRLMNAQKSYKLG
                     VRGEHYRKRSIYGGTIPWVGSHAIDWLSWFSGEKFLSVYASHTNLYNQGHGDLEASAL
                     CHFIMTNEVFASVSIDYLQPKTAKRHDDDRIRIVGTEGVIEIRDRKVFLTNASADGAQ
                     ELHLLEPQNIFEGFIKKIKGEGKCLVSAEESFYNTEACLMARTSADEKKVIYFEKAVR
                     NM"
     gene            4847..5074
                     /locus_tag="PK733_16455"
     CDS             4847..5074
                     /locus_tag="PK733_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ12164.1"
                     /translation="MVTIKDVAREAGVSDTTVSLVFKGSVRVSKETKKKVFAAAEKPN
                     YIPNISAKNLRAGNTNTICLLQAAIGISQER"
     gene            5032..5325
                     /locus_tag="PK733_16460"
     CDS             5032..5325
                     /locus_tag="PK733_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12165.1"
                     /translation="MFAASSNWDISREVKLIKKMIQMRVQGAIICTCEKGSEGIDLLD
                     SFSIPHIAVDSYPDYYQCCGFNDIKSFYKVFRKMMLLTPGEYRNRLAGIKCSS"
     gene            complement(5384..>6180)
                     /locus_tag="PK733_16465"
     CDS             complement(5384..>6180)
                     /locus_tag="PK733_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12166.1"
                     /translation="LPMNEVIEAYKKAKYGALAISNHNKYIPQENEYSGINILDAVEY
                     SKHPHMLQIGVRDYEDISHQDAIDHTVDKGGFVILCHPNWINKGYWDWDKLGQLDKYT
                     GIEILNPVIYTLKGSGLALDTWDYLLSKGKKVFGFGNDDFHQWRDLERSYNVIYAKSN
                     SFQDIKEAVQKGCFYVSDGVALSDFAFDGKTVTVKAGLFVETYVEEYEYKFIGLNGIE
                     LYKCQGKSASFDIDINEPYVRIEATAEHGAKMYLQPLFNCRYFPRF"
BASE COUNT         1797 a         1335 c          990 g         2058 t
ORIGIN      
        1 ggtacaatcc cgcataggag ggtagagtgg cactacctaa ggttccattg atgaaggtgg
       61 gtcggaaacg gaacaactgg agttcatttc tcttccactt gagataggaa ccaccgaact
      121 tccacatgga agcgcctcaa tttattgagg tgaggatgtc actactaaga cttgcattac
      181 atagttctcc gccaacgatc ctttaaaatc attaaatcca tgaggagtat taataataac
      241 atttgctgga atatcgaatt tagagcaaag taatcccgta tccatcatat atgctttaaa
      301 agaagaattg tcagtgtagg cacttaatgg tattttgcct tctgttattc ttatacattt
      361 attaataatt cctgctgttc tcagccaatc caatggtgtt tcgtattcat acgctcttgc
      421 ccctgtcttt atcagcttat actgaaattt acggttctct ttcgccagct gcgctgggac
      481 actgttccaa gctgccatta ttttcgtagt ctcaccgggg gaggcgtatt ttgccatatc
      541 tgctatgtat gcatcattta gcgttttctg tgatgcaaga acaaaattaa agtctttagt
      601 tgtaacatat tctatgacag ctcttggcat accaccaact actatatagg tcttatataa
      661 ttcgagtgca gtttcatgca aagatagagg aatcatgcgc tcgtatgctt cttttatcat
      721 tacgcttaga gcttcattcc cggttgccca taaaaactcc tcaaaatcaa ggggataaag
      781 tgtcatcatt tctactttac ctacgggaaa tgagtacttt tcccgattta aagcaacccc
      841 taacaggctt cccgcagcaa taacatgata ctcaggcgct ttttcataaa aatattttag
      901 ggagagcaat gctctctctg aagcctgtat ttcatcgaaa ataattaaag tgtctccctt
      961 gaaaatactt tgtccgcttt ttacagcaag ctccctgata atacgctctg gatccagatc
     1021 acgttcaaaa accgcaacca cctcacactt attataccca ttttgggcat gcccattcct
     1081 tcgccaaatc caagtttttt gtgcaataaa agccctgccc gtaatcgtta tagggtttgc
     1141 ccttattaag gtcagcgtgt tttacgattt gcggtgaacc atgatataat cattgagaat
     1201 tcctcctcat aagctatcgt cataacgata gctatatata attaaagtat atcgttagga
     1261 cgacactttg tcgactgaaa taatcactta ggggtgcatt tatcaaatct gaagaaatat
     1321 ttaccagggg ctatcgttac ggtgctcaga gccttcacta atgcccctta ccaatatttt
     1381 cttcagataa attaaatgca cccaacaagc gaagtttcgt acttatatca cagtatgttc
     1441 ttttgaattc atcatttcaa taaaatcgct ttcatgtttt gcataagctg tgtatgctgg
     1501 agtacctttg tatagcctgt actttacttt tctaataaat ggcggttcta aatcaagtaa
     1561 ttgaaaagac tctttattag caataaatct atttaaggct ctttcagccc cttctcttat
     1621 tagttcacaa gccctttgcg gatggtaatg aatagcagct aaatttctgt ttctataagc
     1681 ctcaccatca cacttatctc ctgagccggg atttagtcct tttttaaccg ctacagtttc
     1741 gattccatta atgagccctg aagcttcttt agtaaaggct tcatcacctg aaccaaaaat
     1801 tgatcttact cctaagcttg ctgcacacat agccatttgt ccaaattccc ctaccgatat
     1861 tccgtttatt ttgtagtcta aaacattgaa ccatcctgta tgtgctatat gggcaaaagg
     1921 tgttcccgct tttgcatgct gtccaaccca tgcaactgca tcaaaggttt cgtccaggtt
     1981 gaagggataa ggtttaggga aacctctaat aaacatcgtt cttttatcca gcagggattg
     2041 gttaatacct cccgctccat gaccgtctgc tacaaatatt tcggtagccc ccgcagtaaa
     2101 aaagccctca actgctgcgt taatttccat tgtcagcaat cttttgccct cctcgtaata
     2161 cctgccatca ggcatcaccc aatcatcgtg gtttattaca ccacatacac cttccatatc
     2221 agtcataata aaaattttca tggtagacct cccttcttta tttcaccttt cctatgtact
     2281 acctctgtat cctgcctgta ccaccgctct ttatcagact ttttacttca tcagctgtta
     2341 cctgattata gtcaaactca atagtatgct ttagacatga agctgccaca gcaaattcta
     2401 tagcatcctg ggaactataa cctgatacca gactgtagat gagggcacca ccaaaggaat
     2461 ccccaccgcc taccctgtca acaatgtgaa ttagatagtt tctggaaaag cagcactccc
     2521 catcagtgta aagcataccc gcccagttgt tgtcatttgc agtgatgctt cctcttaatg
     2581 taatggctac ctttttacag ccgaatcgtg atgaaatctc ctgtgctacc tgacgatacc
     2641 gcttatgatc cagttgtcct ccatcaacat cggtatgatc cgccttaatg ccaaaaacct
     2701 tttctgcgtc ttcttcatta gaaatacaaa catcaacata tttaattaat tcactcataa
     2761 cttttccagc cttttcactg gaccacaggt ttttcctata atttaaatcg cagcttactg
     2821 taattccttt ttcctttgcc catttacatg catcaaggca aatttctgca gtaccatcag
     2881 aaagtgccgg agttataccg gtaaagtgaa accatgaggc tccatcaaaa attttgttcc
     2941 aatcaaagtc ctgccttcca gctgtagcta tagaggaata ctttctgtcg taaattacct
     3001 tggaagccct ttgagatgcc cctttttcag caaagtatat tcctagtctt tcaccgccat
     3061 aaactatatc cgaaacatca acattgtatc cccttaaatc ttttactgct gcttttgaaa
     3121 gatcgttttc gggtaatttt gtaacaaatg ctgaatttag accataattc gcaagagaaa
     3181 ccgccacatt tgcttctgct cctccatatg aaatttcaag tttctttgcc tgacctaatc
     3241 tgagatatcc ctccgggttc agcctagcca ttatttcacc aaaacaaatt atttttccca
     3301 ttatttctta cctctcttct caaaatatat ttctgctatg atgttgttat tatagctatt
     3361 attgtagctg ttattatagt tgttattata gctattattg tagctgttat tatagttgtt
     3421 attatggcag ttattgtagc tgttattata gttggcaagt atatttttgt gccaatgccg
     3481 caagtttcac atatcatccc aatttattag agaaattttt gctgcatttg tattcaataa
     3541 ctatgctcaa atactaattt atattttaca tatttctaac agctttttca aaataaatta
     3601 cttttttttc atctgcagaa gtccttgcca taagacatgc ttcggtgtta taaaatgatt
     3661 cctcggcact aacaaggcat ttaccttctc ccttaatttt ttttatgaat ccttcaaata
     3721 tgttttgtgg ttccaataaa tggagctcct gtgcgccatc tgcagatgca tttgtaagaa
     3781 acactttccg gtctcttatt tcaataaccc cttctgttcc tacaatacga attcgatcat
     3841 catcatgcct tttagctgtt ttaggctgta aataatctat gcttacagaa gcaaatactt
     3901 catttgtcat aataaaatgg catagtgcag atgcttctaa atctccatgt ccctgattat
     3961 aaagatttgt atgtgaagca tatactgaaa gaaatttttc cccgctaaac caactcaacc
     4021 aatctattgc atgacttcca acccatggta tcgtacctcc atatatgctt ctttttcggt
     4081 aatgctcgcc tcttactccc agcttgtaag acttttgtgc attcataagt cttatttctc
     4141 ctattgagcc ttccttaata gatttatgag cagtaaggaa ccacggagtt gcccgaatgg
     4201 ctaacattgc tgatagatga gcaccggatt gtttataaat attttttagt gtttcaagat
     4261 cttctaatga tgtagccacc ggtttatcca tgaaaacatg tattcctctt tttagagcat
     4321 caccggctac ctttgtctga tcagcgaaat agcagcatac aactgctatt tcgggcttta
     4381 tttcatccaa catattgcag taattatcaa agtattgcac tgaagggtat tctttactta
     4441 gcctttcata aagagaagac acattctcac ctaaagaccc aggtgcaata cctgctattg
     4501 atatttcttt tttatttttg ataccatcaa gagcaatacc gtaatgacct gtagatccaa
     4561 taatacatat tttcataaac tatcccctcc ttatgtagtt aattttcttc attattcagt
     4621 ttactagtaa ataagggaaa aaacaagagt aatgctactt atttatcaaa gcaactcaga
     4681 taccactttt atcacagcaa acccattgac aaattaagta attcccttta tgctataatt
     4741 agtttgacgt caaacctact atgtcataaa taagaaaaaa tttattacct ggatataaac
     4801 caaaatttaa agtaccggca ggagactgac aggagttaat aatactatgg ttactataaa
     4861 agatgttgcc cgtgaagccg gggtttcaga tactactgtt tccttggtat ttaagggaag
     4921 tgtccgtgtt agtaaagaaa caaagaaaaa agttttcgca gcagccgaaa aaccgaatta
     4981 tatacctaat atttctgcta agaatcttcg tgcaggaaac acaaacacta tatgtttgct
     5041 gcaagcagca attgggatat ctcaagagag gtaaaactga taaaaaaaat gattcaaatg
     5101 agagttcagg gtgcaataat atgtacttgt gaaaaaggca gcgaaggcat tgatctcctt
     5161 gattcattct ctattcccca tattgctgta gattcatatc ctgattacta tcaatgttgt
     5221 ggattcaatg atatcaaatc cttttataaa gtgtttagga aaatgatgct cttaactccc
     5281 ggagagtaca gaaacaggct agccggtatt aaatgctcaa gttgatattg cattaaatct
     5341 ataggctgta gattgtctgg ttcatggagt ctgttattat agtttaaaat ctcggaaaat
     5401 atctacagtt aaataatggc tgtaaataca ttttagcgcc atgttcagca gttgcttcta
     5461 tacgcacata gggttcattt atatctatat caaaactagc agatttaccc tggcatttgt
     5521 ataattcaat tccatttaag ccaataaact tgtattcata ttcctcaaca tacgtttcca
     5581 caaacaagcc tgctttcact gtaactgtct tcccgtcaaa agcaaaatca gacaatgcca
     5641 ctccgtcgga aacatagaag catccttttt gaactgcttc tttaatgtcc tgaaaactgt
     5701 ttgattttgc ataaattaca ttatagctgc gctccagatc acgccattga tgaaaatcat
     5761 cgttaccgaa cccaaagact tttttacctt tagataacag gtaatcccat gtgtcaagtg
     5821 ccaaccctga tcccttaaga gtatatatga caggattcaa tatttcgatg ccggtatact
     5881 tatcaagttg gcccagttta tcccaatccc agtaaccttt attaatccag ttagggtgac
     5941 acaaaataac aaatccgcct ttatcaactg tgtgatcaat tgcatcctga tgagatatat
     6001 cttcatagtc tcttaccccg atttgaagca tatgggggtg cttgctgtac tctacagcat
     6061 ctaaaatatt aatgcctgaa tactcatttt cttgtggtat atatttattg tgatttgata
     6121 ttgcaagagc accatattta gcttttttgt atgcttcaat aacttcattc attggcagtt
//
LOCUS       DAOSDD010000246         6175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4577_length_6175_cov_3.400327, whole genome shotgun sequence.
ACCESSION   DAOSDD010000246 DAOSDD010000000
VERSION     DAOSDD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4577_length_6175_cov_3.400327"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="PK733_16470"
     CDS             <1..1539
                     /locus_tag="PK733_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12167.1"
                     /translation="IIDTAKRKRFINTGIITSLIIVTGFIGIVLMGNQEIWTSADFGT
                     NRLYTLIALTATVTVLVISGVLLLFFIKNKINKKYLNLMKKKSSLDIYIDNLEEKLNY
                     RNNGFNNILNSYGAADLKELFKIRAALDEKKMKLKELEILKKKAAELRNSVSLHIKRA
                     SNLMNKNIDGAKELEREIEKLTEDIDANRSQLKCFVDDLNVKSKNLLTKSLNCKSELM
                     AVNSINHKSKLLAGSFNHENELLVNNLNCELEKLVNNLNRNMEWFIDNAKEERGYLPS
                     SLNQGKKHEITLNSIRETEQNLDACSEKVREEEKALALRLKECETIISTSGYDDELLQ
                     KAIEEIEELESEKKKLEDIQFSLSKALDILTEASLEIQKDYMPVLNDRMSYYINEITN
                     GRYKDLRADNKLMLKAEDSESVNIVPVLQLSGGTVDQMYLALRFAMSDLISQKEENLP
                     LIMDEVFAQFDDVRIEESMKLLNEITKNRQVIMFTCKCREVDIAREICKNANIFNLML
                     QFVQ"
     gene            complement(1516..2919)
                     /locus_tag="PK733_16475"
     CDS             complement(1516..2919)
                     /locus_tag="PK733_16475"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ12168.1"
                     /translation="MDILFLSVSTGGGHIKAADALKEYVEKNYLGSRCQIVDTFKYIS
                     PFIDRFVVGGYLNIINKIPLLYGSLYKLSEHNKNIIDATYIISSLFSYKLIPLIQQFK
                     PSIIVCTHTLPLQMVSYLKQKKIISVPVTAIVTDYVNHPFWKLENVDALIVPHDKIRF
                     EMIQAGIPENIIYPYGIPLSEKFLKSHSREKVMTSLGLEDKLTGLIMGGSLGIRSVYN
                     SFEMLIQCKREMQIIVVTGKNTSLKKKMEKQLHFYSNTGTKINTTANAAIDIGTDIDA
                     DTGIDADTGIDIDVDICASNTDTNTSTNTSINTRINASTTSYAGTYTNTSAYTSTCSN
                     TSDCTNTYGSTCIYTINNKNVKIFGYTNIIPELMDISNFIITKPGGMTISEALVKELP
                     IFLTSPIPGQEERNCQFLVNIGAAVCIQPENNLDHVLANILDNASKIKNVKEIAKALA
                     RPNSCRDTAMLLDKLQH"
     gene            3210..3563
                     /locus_tag="PK733_16480"
                     /pseudo
     CDS             3210..3563
                     /locus_tag="PK733_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
     gene            3787..5178
                     /gene="ypeB"
                     /locus_tag="PK733_16485"
     CDS             3787..5178
                     /gene="ypeB"
                     /locus_tag="PK733_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HOJ12169.1"
                     /translation="MGFREKLQDLKRRLSDRKMYSIVIAVIAAISIWGIYQYKHAASL
                     RQQLDNQYNRAFYDMIGYVNNVETLLIKSLITSTPEKTSATLQEAWRQANFAQAYLGQ
                     LPVSQHILANTSKFLSQVGDFAYSLNNSTMGGKNISDEEYKTIENLYGYSAALGKSLN
                     ELQGQLASGRIKWGALAQQGPGIFSRTSANLTAQQFENIDKTFQEYPRLIYDGPFSDH
                     LVTAEPKGVTGEVLTAEQAKQKVVEFFGKDKAASVENTGKNDTGPINTYSFTVKFKNV
                     PDDQTAVIDMTQKGGHPFWMIYNRPVNEKKIDIEKAKSMGKSFLESRGFKDMADTYYL
                     SEDNTATINYAYKQNDVIVYPDLIKLKIALDTGEIVGFEAKGYLSSHRVRDIPKPVIT
                     MEEARAKINKKVNIMSAGLAIIPTDYKTELFTYEFKGKFNNQDFLVYINAVTGREENV
                     LMIVNTPNGILTM"
BASE COUNT         2230 a          839 c         1184 g         1922 t
ORIGIN      
        1 ataatagata ccgctaaaag aaaaagattt attaatacag gaattatcac atctctgata
       61 attgtaacag ggtttattgg aatagtttta atgggaaatc aagagatttg gacatctgca
      121 gattttggaa caaaccggtt gtatacctta atagcactaa ctgctactgt aacggtatta
      181 gttatatcgg gtgtattgtt actgtttttt ataaaaaaca agataaataa aaaatatttg
      241 aatcttatga aaaaaaagag ctctctggat atatatatag ataatttaga agaaaagtta
      301 aattaccgta ataatggatt caataatatt ttgaatagtt acggcgcagc ggatttaaaa
      361 gaacttttca aaataagagc tgctctggat gaaaagaaaa tgaaattgaa ggaattagaa
      421 attcttaaaa aaaaggcagc tgaattgaga aatagtgtga gtcttcatat aaaaagggct
      481 tcaaatctta tgaataaaaa tatagacgga gcaaaagagc ttgaaaggga aattgagaag
      541 ctcacagaag atatcgatgc gaacaggtca cagcttaaat gttttgttga tgatttaaac
      601 gtgaaaagta aaaatctgtt aacaaaaagc ttaaattgta agagtgagct gatggcggtc
      661 aatagcataa atcataaaag taagctgttg gccggtagtt ttaatcacga aaatgaactg
      721 ctggttaata atttaaactg tgaattggaa aagttggtca ataatttaaa ccggaatatg
      781 gagtggttta ttgataatgc gaaggaagaa agaggatatt tgcctagtag cttgaatcag
      841 ggaaagaaac atgaaataac cttgaatagt ataagagaaa cagaacaaaa tttggatgct
      901 tgctctgaaa aagtcaggga ggaagagaaa gcattagctt taaggttaaa agagtgtgag
      961 actataatta gtacttcagg ttatgatgat gaactgttgc aaaaggctat tgaagaaata
     1021 gaggaattgg aaagcgaaaa gaagaagctt gaggatatcc agttttcctt gtctaaagct
     1081 ctggatattt tgactgaagc tagtcttgaa atacaaaaag attatatgcc ggtcttaaat
     1141 gataggatga gttattatat aaatgaaatt acaaatggga gatacaaaga tttaagggct
     1201 gacaacaagt taatgctaaa ggctgaagat tctgaaagtg tgaatatagt tcctgtattg
     1261 cagcttagtg gcgggactgt agatcagatg tatcttgcct tgaggtttgc aatgtcagac
     1321 ttaatttcgc agaaggaaga aaacctacca cttataatgg atgaagtatt tgcccaattt
     1381 gatgatgtaa gaatcgagga atctatgaaa ctattaaatg agataactaa aaatcgacag
     1441 gtaatcatgt ttacctgtaa atgcagagaa gttgatattg cccgggaaat atgcaaaaat
     1501 gcaaatattt ttaatttaat gttgcaattt gtccaataac attgccgtat cacggcatga
     1561 attgggacgg gcaagcgctt ttgctatttc ctttacattt tttatttttg aagcattatc
     1621 caatatatta gctaaaacat gatctaaatt attttcaggt tggatgcata ctgccgcacc
     1681 aatatttaca agaaattggc agtttctttc ttcttgaccg gggattggtg aagttaaaaa
     1741 tatgggcaat tccttcacta atgcttcaga tattgtcata cccccgggtt ttgttatgat
     1801 aaaattcgat atgtccataa gttccggtat tatatttgta tatccaaaaa ttttgacatt
     1861 cttattgttg attgtataaa tgcaagtact gccataggtg ttagtgcaat cactggtatt
     1921 gctgcaagtg ctagtgtaag cactggtatt ggtataagta ccagcgtaag aagtggtact
     1981 ggcattgatt ctagtattga tgctagtatt agtactagta ttggtatcgg tattactagc
     2041 gcagatgtca acatcaatat caatgccggt atcagcatca atgccggtat cagcatcaat
     2101 atctgtacca atatcaatgg cagcattagc agtagtatta attttagtac cggtattgct
     2161 gtaaaagtgc aattgctttt ccatcttctt cttaagactg gtatttttac ctgttacaac
     2221 aattatctgc atctctcttt tacactgaat tagcatttca aaggaattat aaacagacct
     2281 tataccaagg cttcctccca ttatcaatcc cgttaattta tcttcaagac caaggcttgt
     2341 catgactttt tccctgctat ggcttttcag aaatttttca gaaagaggaa taccataagg
     2401 atatataatg ttttcgggta ttcctgcttg tatcatttca aaccttattt tgtcatgggg
     2461 tacaattaac gcatctacat tttccagttt ccagaaagga tgattaacat aatctgtaac
     2521 tatagcagtt acaggtacgg agattatttt tttctgtttt agataagaaa ccatttgtag
     2581 gggtaatgta tgagtacata caataattga aggtttgaat tgctgaatca atggtattaa
     2641 tttgtatgaa aagaggctgc ttataatata tgtggcatca ataatatttt tgttatgttc
     2701 tgatagttta tataaactgc catataaaag ggggatttta tttataatat taagatatcc
     2761 tccaactaca aatctatcaa taaaagggct tatatactta aaagtatcta caatttggca
     2821 tcttgagccc aaataatttt tttcaacata ttcctttaaa gcgtctgctg cttttatatg
     2881 tcctccgcct gttgaaaccg ataaaaataa tatatccaaa ttattttcct ccctgtaaac
     2941 aattatttac ttgaagcttg aaactaatac agatatgtat atttttttct aaaatacaaa
     3001 aaatatcagt tgtaattaga aattaatacg tttctgtaac gtttccatag ccttgcagaa
     3061 aggaggacga agtgtttttg agaaaattta gagctaataa tataaatgtt aatgctatat
     3121 ttattacagc tgtaatttct gtacttttga taataatagg gctaataggt attgttcaac
     3181 cggacctggc ctatgctcag ggaggagcgt caaatgagca gctcctggca agagctatta
     3241 atggagaagc cagaggagaa ccctacgaag gacaagtagc agttggagca gtaatattga
     3301 acagaaccag ggatccaagg tttcccaaaa caatagccgg tgtaatatat caaccgggag
     3361 cttttacagc agtatccgac ggccagataa atgtttcaat agaacaaaat tcaactgtgt
     3421 taaaagcggc cagggatgct ttgaatggtt gggatcctac ggaaggatgc gtatactatt
     3481 ggaatcctgc aacagcaacc agtaagtgga tttggagcag gcaaattgta aaacagatag
     3541 gtaagcatta ttttggaaaa taaattagaa gttaagaaag aagccgtttc cgcttcaggt
     3601 tgggtgatat tttcgaaagc tttaatttat tatgctttcg gaaaagttac actgctgtaa
     3661 atattgcaac tggaaatgat ttgctactta gatcattaaa taggaaatag gttattaatt
     3721 ggcgggaagc taaatttata aacaaaactt ttagaagtaa atttgaaata aaatggaggt
     3781 ggtatcttgg gttttagaga aaaacttcaa gatttaaaaa gaagactaag tgatagaaaa
     3841 atgtatagca ttgttatagc ggtaattgct gctatttcaa tatggggaat ataccagtac
     3901 aagcatgctg ccagtttaag gcaacaactg gataatcaat ataatcgtgc attttatgat
     3961 atgatagggt atgtcaataa tgttgaaact ttgttaataa aatccttgat aacttcaacc
     4021 ccggagaaaa catctgccac tcttcaggaa gcatggaggc aggctaattt tgctcaggct
     4081 tatcttggtc aattacctgt ttctcagcat atattggcaa atacttcaaa gtttctttca
     4141 caggttggag actttgcata ctctttaaat aacagtacaa tgggtgggaa aaatatatca
     4201 gatgaagaat ataaaacgat agaaaactta tatggatatt cagcagctct tggaaaaagc
     4261 cttaatgaac ttcagggaca gcttgcttca ggtaggataa aatggggagc attggcccaa
     4321 caaggccctg ggatatttag tagaacttct gcaaatctaa ctgcacaaca gtttgaaaat
     4381 atcgacaaga catttcagga atatccaaga cttatatacg acgggccttt ttctgatcat
     4441 cttgttactg cagaacctaa aggagtgaca ggagaagtat taactgcaga gcaggcaaaa
     4501 caaaaagtag ttgaattctt tggaaaagat aaagcagctt ctgttgagaa taccggaaaa
     4561 aatgacaccg gaccaataaa tacttatagt tttactgtaa agtttaaaaa tgttcctgat
     4621 gatcagacgg cagttattga tatgacacaa aagggtggac atcctttttg gatgatttac
     4681 aacaggccgg taaatgaaaa gaaaattgat attgaaaagg ctaaaagcat ggggaaatct
     4741 tttttagaaa gccgtgggtt taaagatatg gcggacactt attatctaag tgaagataat
     4801 actgctacaa taaactatgc atataagcaa aatgatgtaa tagtatatcc ggatttaata
     4861 aagcttaaaa tagctcttga tacaggggaa atagtgggtt tcgaagcaaa agggtatctt
     4921 tcatcccaca gggtaaggga catcccgaaa cctgtaatta caatggaaga agccagagca
     4981 aaaataaata aaaaggtaaa tataatgagt gccggcctcg ccataatacc cactgactat
     5041 aaaacagagc tatttaccta tgaatttaaa ggcaaattca ataatcagga ttttctggtg
     5101 tatattaatg cagtaacagg cagggaagaa aatgttttga tgatagtaaa tacaccaaat
     5161 ggaattctga caatgtaaac agtaagcagg aggtgcactt tatttttata aaggaatggc
     5221 attaatgggt attgagaatc tggtggccag cttcatttat aagttatgag gtgtataagt
     5281 ttattaggtg tataaggtta tacttatata agtgtattaa tgcgtatact gttactgttc
     5341 tcggtcactc tttttgggac taagacagaa tacaagtcac tctatttggt actggtaaag
     5401 taagatttgc gatcctcgca atggtcatgc ctgttaagat ttaacggaca gacagacttt
     5461 tttaattgca ttttcagtat caaataaagt aattattcca gtcccaaata aaaaaactgg
     5521 taacacctga aaacatgatg atatgcatga ttatttttga gaacaaaata gctatatttg
     5581 actataatca agaaaagcaa taagctatac ttaaatctag tacatgcgaa aattattaac
     5641 aaatatttag attattcaat atccatatta tgaaatatta tgtaaatata tacaattgaa
     5701 taatttaatg ataaaattta ttaatttgat atatgattaa acaaaaactt atttttaatt
     5761 aaaaaagtca ttatgtttac gagagacttg gaacaaaaat gtaaatatgg tataacaaat
     5821 taggattaaa gaatcaagat tagcaagact caaactatta gttctacaaa tgaaaagtga
     5881 aaccttttat agtaaaattt ttttgaaatg ttttatgaga tgattaaata ataacagtat
     5941 taagctgtat tatttggtta caattatata aacaataaat aaccgataaa ttttataatt
     6001 ttttataggg taaggtgaat gtatatgagg ttatagaata acttataatt cacacttaac
     6061 tataattatt aattattaaa gcagtaaaaa taattactgg tagtgtcaaa taagtaatga
     6121 aaaaataaga agcaatgcat gaaaagcgtg aattttgaac cttgatatat tgtta
//
LOCUS       DAOSDD010000247         6170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4580_length_6170_cov_6.607522, whole genome shotgun sequence.
ACCESSION   DAOSDD010000247 DAOSDD010000000
VERSION     DAOSDD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_6170_cov_6.607522"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..733
                     /locus_tag="PK733_16490"
     CDS             167..733
                     /locus_tag="PK733_16490"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOJ12170.1"
                     /translation="MHSLLGTAGIGWKSYVNQYHMLGYKKILGSRLQYFIKSGELELG
                     CIQFSTSSWALKDRDDWIGWDIEDKKQRLHLIINNTRYLISPWMQIPNLASKGLSLAI
                     KQIQVDWLREFCYAAVLLETFVDSQHFNGTCYKASNRIYLGQTKGQDRNSTNHKQNRS
                     KKDIYVYPLQKDLRSCIKGETPYYEGKI"
     gene            940..1749
                     /locus_tag="PK733_16495"
     CDS             940..1749
                     /locus_tag="PK733_16495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12171.1"
                     /translation="MNIPKKLEFFRQNPVVNWHEHVQSDKNKRLDCQQLDSLVRDSEL
                     VGIDINVVSRPYATKHFGPEVMEFNNNIVAEAMSLYPGKIQGFAFIDPIHGKYAVREI
                     DRCVQELGMIGVKLYDQFTLCDDMQNPIIERCIELDIPILMHAGKACQHPLKKPLESD
                     STHFIAAAKKYPEAVFIMGHIGGGGDWNWQLKGMEKFNNVFTDISGSVHDDMLIEETV
                     RVMGANRVLFATDGSFSSSIGKLLDADINDEDKKIIMNNPSFIKYLNRGRK"
     gene            1749..2540
                     /locus_tag="PK733_16500"
     CDS             1749..2540
                     /locus_tag="PK733_16500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12172.1"
                     /translation="MIIDVNTYIGNYPFRPIRNNSPREFVNLMDRHDIDKACVSSLSG
                     IYYRDVIKGNYELLEGIAPYQDRLIPVCNINPIYAKSMSDFCECVEKLGFKGVKLFPR
                     QHGYELSNEKSVELLNKAAEYGVFVQLPLYIEDLRQRHPMDIDYPLSAQEIMDAALLA
                     PKTDFILSNYSFSTFKDVFHDISNKRQGHIYYDISRVECLKRNFIADMLEFTDIEHLL
                     FGTGAPLEYIDVQLVKLSYFPQTLGLTEKQIELIKSGNMIKLLRM"
     gene            2786..3931
                     /locus_tag="PK733_16505"
     CDS             2786..3931
                     /locus_tag="PK733_16505"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HOJ12173.1"
                     /translation="MGRKRLYMGSFFDYFTIIFDPRQKGKVRHKLIDVLFITVAATVC
                     RCDEWEEMEEWAVAKEEWLRQYLELPNGIPSWYTIERVLDVIDPKQFEKCFVEWMKEV
                     TQFGKGDIIAIDGKTMRGTADKSNAKKAVHIVSAWCSSDKLVLGQVKTDEKSNEITAI
                     PELLDLLFIKGAIVTIDAMGCQKDIATKIVKEKKADYVLSLKDNHEILKGEVEEYFKD
                     AEKTNFKDDNILQYSTLERGHGRIEERLYYYSTDISWMDARKDWTKLTGIGMVIRRTE
                     VNGEKTEERAYHLGSVKTAEEYARGVRMHWGIESTHWSLDVTFREDASRTRKGCAPQN
                     LALLKRMVLNMVKKDNERYPKKSLKRRRFMASLYNDYLEYIFAINFR"
     gene            4433..4615
                     /locus_tag="PK733_16510"
     CDS             4433..4615
                     /locus_tag="PK733_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12174.1"
                     /translation="MSLSTIRRMAYATGYQLGKNKDYFYLVDKRGNVIAQGSIFEIAA
                     TVKQHYKDNYNPLYAA"
     gene            4694..4948
                     /locus_tag="PK733_16515"
     CDS             4694..4948
                     /locus_tag="PK733_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12175.1"
                     /translation="MSELSNIVVLIVKYSILLFLILIVTYIATTIAWISAIEAVKENV
                     DDADDKEKILKQRYIKKEPEKKASSCNIITSIEPEKRKVG"
     gene            4951..6108
                     /gene="dnaN"
                     /locus_tag="PK733_16520"
     CDS             4951..6108
                     /gene="dnaN"
                     /locus_tag="PK733_16520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ12176.1"
                     /translation="MSMSFNIARKEFLAAVNSAIRAVPGKTSQPILEGLLIEASGEEI
                     SITGNNLEIGIKSKAKATVENEGSIVTDARLLHNILKLLPDADTNFMLKENNEILLTC
                     LLSEYTLKGVEASSFPQIPELEALQEIKLSAATFKDMLSKTLFAAGKDENRPILTGVL
                     LEVNSNNLNAVAIDGFRLAHSNSNTEGKLKNVTDTSIVIPSKTAIELAKTLPYKSDID
                     VNISIQSNIALFEYENTKITSKLLEGQYLNYKTIVPKEFGTMFVINKKDFLDSVERSM
                     LIASTGSSTNPLRLEILPEKSTIIFTAKSSTGNMREELKIQGKGPNVTIHFNPLFIAE
                     GLRAINDDVVAIGTNDPLNPMIIQVPDYVYMILPIRPPASFEKEKEEKVAA"
BASE COUNT         2191 a          938 c         1247 g         1794 t
ORIGIN      
        1 atagaagtga tgtaaagttt cgtatcgaaa ccacagcttc catctgcttc attcatatga
       61 ggaggattcc cgctgcgttt cctaaattat ccataaccga gcttgcatcg actatatgtg
      121 agcttttaga ttggattacc ccatcgggtg gtgcaaaaag ggatcaatgc atagccttct
      181 tggaacagct ggaatcggat ggaaatccta tgttaatcaa tatcatatgc taggatataa
      241 aaagatactt ggatcacgac ttcaatattt cataaagtct ggtgaacttg agcttggctg
      301 tatacaattt tcaacctcat cttgggcatt aaaggaccgt gatgattgga tcggatggga
      361 tattgaagat aaaaagcaac gccttcacct aataatcaat aataccagat acttaatttc
      421 cccttggatg cagatcccga atctggccag taaaggatta tctctggcga taaaacagat
      481 acaggtagat tggctgcgtg aattttgtta tgctgctgtt ctactagaga catttgtaga
      541 cagccagcat tttaatggaa catgttacaa ggcatctaat aggatatacc ttggtcaaac
      601 caaaggtcaa gacagaaata gtaccaatca taagcaaaat agatccaaga aggatatcta
      661 tgtatatcct cttcaaaaag accttagatc ttgcattaaa ggcgaaacac cgtattatga
      721 ggggaaaatt taagatgggc attgatgagc gtttccttga tggatttaaa gatgcactca
      781 atagtataaa ggaataaaag attaaaaata aaaaggatta ggataatgta tattttttct
      841 atatttcaat ggcagatttc tgtaacccaa atgaggtaat tgtggatctt agtaacccta
      901 aaatggttgc tacacatata tgaggaggta attcttcaaa tgaatatacc taaaaaactt
      961 gaattctttc gccagaatcc tgttgttaac tggcatgaac atgttcaaag tgataaaaat
     1021 aaaaggctgg actgtcaaca acttgattct ctcgtcagag attcagagct tgttggaata
     1081 gatatcaatg ttgtttcacg tccctatgcg acaaaacatt ttggacctga agttatggaa
     1141 tttaataaca atatagttgc tgaggccatg tcactctacc ccggtaaaat tcaagggttt
     1201 gcttttattg acccgattca tggcaaatat gcagtacgtg agattgatcg ttgtgtacag
     1261 gagctaggca tgataggtgt aaagttatat gatcagttta ctttatgtga cgatatgcaa
     1321 aatccaataa tagagagatg tattgaactg gatattccca tacttatgca tgcaggcaag
     1381 gcatgccagc atccgcttaa aaaacctctt gaatctgaca gcacccattt tattgctgct
     1441 gctaaaaaat atccggaagc agtatttatt atgggacata tcggcggtgg cggagattgg
     1501 aattggcaac ttaagggtat ggaaaagttc aataatgtct tcaccgatat aagcggaagt
     1561 gtccatgatg atatgctgat tgaggaaact gttagagtta tgggggcaaa ccgtgtttta
     1621 ttcgcaactg atggttcgtt ttcatcaagt ataggaaagc ttcttgatgc agatatcaat
     1681 gatgaggata aaaaaattat aatgaataat ccatcattta taaaatattt gaacaggggg
     1741 cgtaaataat gataatagat gtaaatactt atataggtaa ctatccattt cgtccaatta
     1801 gaaacaattc ccctcgcgaa tttgtaaatt taatggatcg acatgatatt gataaagcct
     1861 gtgtttcaag tttgagcggt atatattatc gtgatgttat taaaggcaac tatgagctct
     1921 tggagggaat tgctccatac caggatcgtt tgattcctgt atgcaatata aatcctatat
     1981 atgcaaaatc catgtcagat ttttgtgagt gtgttgaaaa gcttggcttc aagggagtaa
     2041 aactttttcc tcgacaacat ggctatgaac tctccaatga aaaatcagtt gagcttttaa
     2101 ataaggctgc tgaatatgga gtttttgtac agcttccact ctatattgaa gatttgcgtc
     2161 agcgtcaccc catggatata gattatcccc taagtgcaca agaaattatg gatgctgcat
     2221 tgcttgcccc gaagacagat tttattctgt caaattacag cttttcaacc tttaaagatg
     2281 tttttcacga tataagcaat aaacgtcaag gtcacatcta ttatgacatc tcaagggttg
     2341 aatgtctgaa aagaaatttt attgctgata tgctggagtt tacagatatt gagcatcttc
     2401 tctttggtac aggtgcacct cttgaatata ttgatgttca gcttgtaaaa ctttcatatt
     2461 ttccacaaac actaggactt acagagaaac aaatcgaatt aataaaaagt ggaaatatga
     2521 tcaagctgtt gagaatgtga gtttttaatg tgatacatat tagtaagttt gaagctgaag
     2581 tacggttatt gttctttttt aggaagtgaa gaaccatagt atataatatt atataaatta
     2641 aattttgcat ctttcttatc tgaacctatt tccacggcaa aagcatgaaa gaatatatta
     2701 ccatggaata aattacagct aaactttaga agacatatat attaagctat tgtctataga
     2761 gaataataag gatgaggtca aaactatggg cagaaagcgg ttatatatgg gaagtttttt
     2821 tgattatttt actataatat ttgatccaag gcaaaaagga aaagttaggc ataaattgat
     2881 agatgtactg tttattactg tggcagcgac tgtatgcagg tgtgatgaat gggaagagat
     2941 ggaggaatgg gctgttgcca aggaagagtg gctaaggcaa taccttgagt tgccaaatgg
     3001 aattccatcc tggtatacga tagagcgtgt attggatgtt attgacccta agcaatttga
     3061 aaagtgtttt gtagagtgga tgaaggaagt tacacaattt gggaaaggcg atataatagc
     3121 tattgatggc aaaacaatgc gaggaactgc agataaaagt aatgccaaaa aagcagttca
     3181 tattgtaagc gcatggtgca gtagtgataa actcgtcctt ggacaggtaa agacagacga
     3241 aaagagcaat gaaattacag caataccaga gcttttggac ttgttattta taaaaggagc
     3301 cattgttacc attgacgcca tgggttgtca gaaagatatt gcaacaaaga ttgtaaaaga
     3361 gaaaaaagca gattatgtac tgtctttaaa ggataatcat gaaatactaa aaggagaggt
     3421 agaggaatac tttaaagatg cagaaaagac aaattttaag gatgataata ttttgcagta
     3481 tagtacattg gaaaggggcc atggccgtat tgaggagcgt ctttattatt attccactga
     3541 cattagttgg atggatgcca gaaaagattg gacaaagctt actggtatag gtatggtgat
     3601 tcgtaggact gaagtcaatg gagaaaagac ggaagagcgc gcatatcatc taggaagtgt
     3661 aaaaaccgca gaagaatatg cccggggggt gcgtatgcac tgggggatag aaagtacaca
     3721 ctggagcctt gatgtaacct tccgagaaga tgcaagtaga acaagaaaag gctgtgcacc
     3781 acagaattta gctttactaa agaggatggt tcttaatatg gtgaaaaaag acaatgagag
     3841 atacccgaaa aagagcctta agaggagaag atttatggct tctctatata atgattacct
     3901 cgaatatata tttgcaataa actttagata aggtaaatta tgcaagatac tgggtatatc
     3961 cttgaccgga ctacctggtt gatacagggg taatagcata gatagggata tcccgggcgt
     4021 ttctaatacc aaatcatagc aataccggaa tacatttctg aaaaagaaaa gcctaccaaa
     4081 atatttatgc tgaaatggca tttttaaaaa actctgacat cagggtcttg gtaggaaatg
     4141 tattcatgct tttaccgtga acctatttca aaatagactt gctaaaacca taaattaaat
     4201 atataataaa gattgtatta gtgaattatt aaagtacctg ttaaaagata cttaggttat
     4261 gataagaata aatggtctag ggaaaattat attctctaga ccttttttgt ttttaaaaaa
     4321 atatatttat atatgtgcta aaaaaaggga gaagaaccta taagtgaaat cgcttatatg
     4381 gggcttctcc ctttttttgt tataagcgaa caaggaaaaa gggggtgtga taatgtcatt
     4441 atcaaccatt aggcgaatgg cttacgcaac agggtatcaa ctaggcaaaa acaaagatta
     4501 cttttatcta gttgacaaga gaggtaacgt tattgcccag ggttctattt ttgaaatagc
     4561 agctacagta aaacagcatt ataaggataa ttataatcct ctctatgcag cctgataagc
     4621 ctaatagcaa gaaaaaagtc ctgtgaatat taaatattca taggactaat atttaaaaga
     4681 gggtgataag atcatgtcag aactgtcgaa tattgtcgta ttaatcgtaa aatattctat
     4741 actattgttt ctgatactaa tagttacgta tatcgcaacc acaatagcat ggataagtgc
     4801 tattgaagca gttaaggaaa acgtagatga tgcagatgat aaggaaaaga ttttaaaaca
     4861 acgctatata aaaaaagaac cagagaaaaa agcttcatca tgtaatatta tcacaagcat
     4921 agaaccagaa aaaaggaagg tgggataaat atgtccatgt cttttaacat cgcaagaaaa
     4981 gaatttcttg ctgctgtaaa ctctgctata agagcagtac caggcaaaac atcacagcct
     5041 attttggaag gtctgcttat agaagcaagt ggcgaagaaa ttagtatcac aggtaataac
     5101 ctagaaatag gaattaagtc aaaagcaaag gcaacagttg aaaatgaagg aagcattgta
     5161 acagatgcta gacttcttca taatatttta aaattattgc ctgatgcaga cactaatttc
     5221 atgcttaaag aaaacaatga aattttatta acttgtttgc tttctgaata caccttaaaa
     5281 ggcgtggaag catctagctt tccacaaatt cctgaactag aagcactaca agaaataaaa
     5341 ctaagtgctg ctacgtttaa agatatgcta tcaaaaacct tgtttgcagc aggcaaagat
     5401 gaaaaccgtc ctatcttaac tggtgtttta ttagaagtta acagcaataa cttaaatgct
     5461 gttgccatcg acgggtttag acttgcacat agtaattcta atacagaagg aaagcttaaa
     5521 aatgtaacgg acacatcaat cgttattccc tcaaaaacag cgattgaact tgcgaaaaca
     5581 ttgccttata agagcgatat agatgtaaat atttcaattc aaagcaatat tgctctattt
     5641 gagtatgaaa acacaaaaat aacaagtaaa ttgcttgaag gtcaatatct taattataag
     5701 acgatagttc caaaagaatt cggaactatg tttgttatta acaagaaaga tttcctagat
     5761 tccgttgaac gttcaatgtt aattgcaagt actggcagtt caactaatcc attaaggtta
     5821 gaaatattgc ctgaaaaaag cactataata ttcactgcaa aatcaagcac aggtaatatg
     5881 cgagaagagc tgaaaataca agggaagggg ccaaatgtta ctatacattt taacccgtta
     5941 tttattgcag aaggtttaag agcaataaac gatgacgtag tagcaattgg aacaaacgat
     6001 ccattaaatc ctatgataat ccaagtaccg gactacgtat atatgatatt acctattcgt
     6061 ccaccggcat catttgaaaa ggaaaaagag gagaaagtag ctgcataata taaaggtgaa
     6121 ctacatcgtc attaaaatga cgagcttctg gttccctgaa aacaggtaca
//
LOCUS       DAOSDD010000248         6149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4601_length_6149_cov_4.827371, whole genome shotgun sequence.
ACCESSION   DAOSDD010000248 DAOSDD010000000
VERSION     DAOSDD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_6149_cov_4.827371"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1466
                     /locus_tag="PK733_16525"
     CDS             297..1466
                     /locus_tag="PK733_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ12177.1"
                     /translation="MKSATIKDVAKKAGVSISVVSYVLNNNPNVSISDETRTRVLDAA
                     KSLNYKPNSIARSMRTNKSMVIGLATFWDVSDSVFTDVLKGVDSVAERNGYSVTYCNI
                     RNSFNGLKITELYNQRQIDGVILLLHVDPAKSFSEIQFINNIKQSGIPAVIIDGSTED
                     PDMSYVYIDYYGTSYTAVNYLYKLGHRKFCYMLPDINEIHNIQAVQRINGYKDALKNL
                     NLVDYGLYFNQDSVHDIVNLIKPDSNVPANNVSAYDMPVNDVPVNNASVYNAPVNDVS
                     LDNAPVNYASDSDNQASKKPTAVVVNKTRYAVYLLKAFSEHGIKVPDDISVISCNNQN
                     YAEFLTPPLTTIKVPIYEIGEKSADILFDIMKDKPLNIKLKLLNEVIERGSCKER"
     gene            1743..2603
                     /locus_tag="PK733_16530"
     CDS             1743..2603
                     /locus_tag="PK733_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ12178.1"
                     /translation="MSEFKKNLKEYAKSIGVDLIGFAQKERFEGVIPEENPFTIFPEA
                     KTVILLGKRITRGTLRGVEEGTNFGDYSLFGSSWLDDNINSLMCYDITRYLEDRGYEA
                     VPVFPNPTEAKGMGIPVGEGKAAPNVTPNFEYAVVACGLGEIGYCGEILTPEFGPRQR
                     WQMVITDAEIEEDEILKDSICTFCEKCASVCPLGAITGSREIEICGKRMKTGIIDYNL
                     CKKCKNGAFGNRLLKSAKPDRLAALCVRTCVHELDNQKKVKNTFEKPFRKREAWAIDI
                     YDKSKNIKLD"
     gene            2722..3852
                     /locus_tag="PK733_16535"
     CDS             2722..3852
                     /locus_tag="PK733_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12179.1"
                     /translation="MELTSQMIKKKAKDLGIDVIGIGSIDRYVNAPVLMNPKTYFPEA
                     KSVIVIGMRIPRGSYRGIEEGTHWHNYTFYSYNRLNSFFRPRLTYELACYIEDYGWEA
                     VPHYPAVPERAAAQRPVAEGTVPHDVVFSIRLMGVGAGAGEMGHSKVFLTKEFGPRIR
                     LGCILTDAELDPDPIIEPGTICNKCGRCVRDCPGNAIPPVKDEDKKLTVEIGGKKISW
                     GDVHMGRCTLTHHGLNNTVSPFLKKDFPNLEFDAASSDTTEEEAYRLTYPLALGTWSN
                     CHYEENSGSIIKYYKYILSHCGYFAVCGAKGCIRACMDSLEKSKRIENTFKHPFYHKK
                     GWNLTVERVGDKKGVINPFREEGLDKLYPSIREGEQEKSKFK"
     gene            complement(3917..4002)
                     /locus_tag="PK733_16540"
     tRNA            complement(3917..4002)
                     /locus_tag="PK733_16540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3966..3968),aa:Leu,seq:tag)
     gene            complement(4096..4515)
                     /locus_tag="PK733_16545"
     CDS             complement(4096..4515)
                     /locus_tag="PK733_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ12180.1"
                     /translation="MVENLTNESVEVVEIIHGDKLKIADEVIETIAGIEASKVQYVTS
                     MSGGFTDGLAGFLGKKNLGKGVRVEATENEVKVFLSITVEYGCKIHVVAKNVQNIVRT
                     SIEEMTGMKVLEVNVSVAGINFPKEPKEPKIEEQEKA"
     gene            complement(4662..6056)
                     /gene="asnS"
                     /locus_tag="PK733_16550"
     CDS             complement(4662..6056)
                     /gene="asnS"
                     /locus_tag="PK733_16550"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOJ12181.1"
                     /translation="MLMASVKQIYRNQEDYVNKTVIISGWIRTLRDSKAVGFIELNDG
                     SFFKGVQVVFERESIGNFDEIAKLPSGSSIIVEGEIVETPNAKQPFEIKAIRIEIEGI
                     STQDYPLQKKRHSFEYLRTIAHLRPRTNAYSAVFRVRSLVAYAIHQFFQERGFVYVHT
                     PIITTSDCEGAGEMFRVSTLDPENLPLNENGKVDYSQDFFGKQTGLTVSGQLAGETYC
                     MAFRNIYTFGPTFRAENSNTARHASEFWMIEPEIAFADLNDDMELAEDMIKYIINYVL
                     ENAPEEMEFFNSFIDKSLLERLNNVINSEFAHITYTEAINILKNSGHTFDYPVDWGSD
                     LQTEHERYITEQVFKKPVFVTDYPKDIKAFYMRLNDDDKTVAAMDLLVPGVGEIIGGS
                     QREERLDYLEKRMDELGMDRSEYWWYLDLRRYGGTRHAGYGLGFERAIMYITGMTNIR
                     DVIPFPRTPKSAEF"
BASE COUNT         2014 a         1112 c         1127 g         1896 t
ORIGIN      
        1 aaagcagcat tagaaataga acgcaatata tagcatgttc tgcttttttc ttagtatcag
       61 tgaactatag tatgggtgtg tgagggtata aagggtaaat acaaggctgt gtatagccta
      121 tgcaatttat gatttatata tctgtcactg tttttaaaaa tatcttgaca cagctgtgaa
      181 tttattatat attttactta tagaaacgtt tctgttaaat gattggtttc aaaatgctgt
      241 tgctagacta aagcactggc aaaccacaca ggttattgtg attgaaggtg aaatttgtga
      301 aatctgcaac aattaaagat gtagcaaaaa aagcaggggt tagcatatca gtagtttcat
      361 atgtactaaa caataacccc aatgtttcca tatcagatga aacaagaacc agggtccttg
      421 atgcagcgaa aagccttaac tataaaccca atagtatcgc aagaagcatg agaacaaata
      481 aatcaatggt tataggtctt gccaccttct gggatgtatc agactcagta tttaccgacg
      541 tccttaaggg ggttgacagt gtggctgaaa gaaatggcta ctcagttaca tattgcaata
      601 taaggaatag ctttaatggt cttaagataa cagaattata caatcaaagg caaattgatg
      661 gtgtaatcct tctgttacat gtagatccag ctaaaagttt tagtgaaatt caatttatta
      721 acaatatcaa gcaaagtggt atccccgcag ttatcattga tggaagtacc gaagatcctg
      781 atatgagcta tgtatatatt gattattacg gaacttcata tactgccgta aactatcttt
      841 ataaacttgg tcacagaaaa ttttgctata tgctcccgga tataaatgaa atacataata
      901 tacaagcagt tcaaagaata aatggatata aggatgcatt gaagaatctt aatctggtgg
      961 attatggttt atacttcaat caagattctg tacatgatat tgtaaattta attaaacctg
     1021 atagtaacgt acctgctaat aatgtatcag cctatgatat gccagttaac gatgttccgg
     1081 ttaataatgc atcagtttat aatgcaccgg ttaacgatgt gtcgcttgat aatgcaccgg
     1141 ttaattatgc atcagatagt gacaatcaag cttctaaaaa gccgaccgct gttgttgtta
     1201 acaaaacacg ctatgccgtt tatcttttaa aagccttttc agagcatgga attaaagtac
     1261 ccgatgacat ttcggttatt tcatgcaata atcaaaacta tgcagaattt ctcacaccac
     1321 ctttaacaac aataaaagta cccatatatg aaatagggga aaaaagtgcc gatatactat
     1381 tcgatattat gaaagacaaa ccgttaaaca ttaaattgaa acttttaaat gaggtgattg
     1441 agcgcgggtc gtgcaaggaa agatagaatg ttagaacaag ttaagcaata tttttttaat
     1501 ggttatagaa acgtttctat aactagtaaa ggcatcaata tcactaactg tataaatact
     1561 gattactgca aaaaaatatc tgctgctcga aaactgatta ttatatgaaa tctgattatc
     1621 acatgaaatt ttttgactat acgaaaaaca gattttatgt ttgtaaaaca ttttataaag
     1681 cgtaatgatt acataatgac taaaattata aagatgaaat tagtaaaaga aaggggatct
     1741 atatgtctga attcaaaaaa aatctaaaag aatatgcaaa gtccatagga gttgacctta
     1801 taggttttgc acaaaaagaa aggtttgaag gcgtgatacc tgaagaaaac ccttttacaa
     1861 ttttccctga agctaaaaca gtaattcttc tgggtaaaag gataaccaga ggaactttga
     1921 ggggtgttga agagggaaca aattttggtg actattccct gtttggttca tcatggcttg
     1981 atgacaatat taactcatta atgtgctatg acataacaag atacctggaa gacagaggct
     2041 atgaagctgt acctgttttc cccaacccaa ctgaagctaa aggcatgggt attcctgtcg
     2101 gtgaaggaaa agcagcccct aatgttactc caaacttcga atacgctgtt gttgcatgtg
     2161 gtttgggaga gattggctac tgtggtgaga tactcactcc ggaatttggc cccaggcagc
     2221 ggtggcaaat ggtaataacc gatgcagaaa tcgaagaaga tgaaatcctc aaagatagta
     2281 tttgcacttt ttgtgaaaaa tgtgcaagtg tgtgccctct tggtgcaata acaggaagca
     2341 gggaaataga aatttgcggc aaaagaatga aaactggtat tatcgactac aacctttgta
     2401 aaaagtgtaa aaacggtgcc tttggaaata gactgctgaa aagtgcgaaa cccgacagac
     2461 tggcggctct atgtgtcagg acatgtgtac atgaattgga taatcagaaa aaagtcaaaa
     2521 atacttttga aaaacctttc aggaaaagag aagcctgggc tatagatatt tatgataaaa
     2581 gcaaaaacat taaattagat taagccagca aagatagtct taaataactt ggagacagtt
     2641 ctcgaataat tgaagatagt tcttcaaatt gtcaggggac agttcacgaa aataatagaa
     2701 aaaatgtgga ggtgttatat tatggaactt acttcccaaa tgataaagaa aaaagcaaaa
     2761 gatttgggaa ttgatgttat agggattggc agtattgata gatatgtaaa tgcgccggta
     2821 cttatgaatc caaaaacata ttttcctgaa gcaaaatcgg taattgtaat aggaatgaga
     2881 attccaagag gttcgtacag aggaatagaa gaaggcaccc attggcacaa ctatactttt
     2941 tattcatata acaggttgaa ttcatttttc aggccccgtc ttacctatga gctggcttgt
     3001 tatattgagg attatggctg ggaagctgtc ccccactatc ctgccgtacc tgaaagagct
     3061 gctgctcaaa gacctgttgc tgaaggaaca gtccctcatg atgtggtttt cagtataaga
     3121 cttatgggag taggtgcagg agcaggagaa atgggacatt caaaagtgtt cctaacaaag
     3181 gagtttggcc ctagaattag attaggttgc attttaactg atgctgaact agatccagat
     3241 ccgattattg aacccggaac aatttgcaac aaatgtggac gatgtgtaag agattgtccc
     3301 ggaaatgcca taccccctgt taaagatgaa gataaaaaac ttacagtaga aataggcggc
     3361 aaaaagattt catggggtga tgtccatatg ggacggtgca cattgactca tcatggttta
     3421 aacaatacag tgtctccatt tttaaaaaag gattttccca acctggaatt tgatgctgcc
     3481 agttcagata ctaccgagga agaagcttac aggctgacat accctcttgc tcttggaacc
     3541 tggtcaaatt gtcattatga agaaaacagc ggcagcatca ttaaatatta taaatatata
     3601 ttaagccact gcggctattt tgcagtatgc ggagccaaag gctgcataag ggcatgtatg
     3661 gattctcttg aaaaatcaaa acgtattgag aatacattta agcatccatt ttaccataaa
     3721 aaaggatgga acctaactgt agaaagggtt ggtgacaaga aaggagtcat taatccattc
     3781 agagaagaag gtcttgataa actataccca tctatcagag aaggtgagca agagaaaagc
     3841 aagttcaaat gattggatga aatttaaaca aattaaaaag gacttcaata aaaatattac
     3901 gaagtccttt ttaatttggt gcggacgaag ggacttgaac ccacacggct attaaccact
     3961 ggaacctaaa tccagcgcgt ctgccaattc cgccacgtcc gcataatata atagctatta
     4021 gatataaaat ctaaaatact tctaatatac tagcacatga tctcaggatt ttcaataggt
     4081 aattttattt ttttattatg ctttctcttg ttcctctatt ttaggttctt ttggctcttt
     4141 agggaaatta attcccgcaa cactcacatt aacttcaaga accttcatac ctgtcatttc
     4201 ctctatggaa gtccttacta tattttgtac gttttttgct accacatgaa ttttgcatcc
     4261 atactcaact gttatggaaa ggaatacctt cacctcgttt tctgttgctt ctactcttac
     4321 acctttgccc agatttttct ttccaagaaa ccctgccaat ccatctgtaa atccaccact
     4381 cattgaagta acatactgta ctttcgaagc ttcaataccg gctatagttt ctattacctc
     4441 atcagcaatt ttaagcttgt caccatgtat tatttctact acttcaacag attcgtttgt
     4501 caaattttca accataaaga tacctcctgt atttatcctt cattaccatt atatattatt
     4561 tataccgtaa ataacatagt taaacatttt tcttttgttc tttgttctct gtctaccact
     4621 ttataagaac gcctctttat tgttcaccca gcaagctcat tttaaaactc ggctgattta
     4681 ggcgtcctgg ggaatggaat tacatccctt atattagtca ttcctgtaat atacattatc
     4741 gctctttcaa agcccaaacc ataccctgca tgtctggtcc cgccatatct tctcaagtca
     4801 agataccacc aatattctga cctgtccatc ccgagttcat ccattctttt ttcaagataa
     4861 tccaatcgtt cctctctctg gcttcctcca attatttcac ctacacctgg gaccaacaga
     4921 tccatagcag ctacagtttt atcatcatca tttaatctca tataaaaagc tttaatgtct
     4981 ttaggataat cagtcacaaa tacgggcttt ttaaatacct gctctgtaat atacctttca
     5041 tgctctgtct gaaggtcact gccccaatct acaggataat caaaggtatg gccggagttt
     5101 ttaagaatat ttattgcttc ggtatatgtt atatgtgcaa attcagaatt tataacatta
     5161 ttaagccttt ctaaaagact tttatctata aagctattga aaaactccat ttcttctgga
     5221 gcattttcca acacatagtt gattatatat tttatcatat cttctgcaag ttccatatca
     5281 tcattaaggt ctgcaaaagc aatttctggt tctatcatcc agaattcgga tgcatgtctg
     5341 gcagtatttg agttttcagc tctgaaagta ggtccaaaag tatatatatt cctaaatgcc
     5401 atgcaatatg tttcaccggc taattgtcca cttactgtga gaccggtttg ctttccaaag
     5461 aagtcttgcg aatagtccac ttttccattt tcgttcagag gtaagttttc agggtcaagg
     5521 gttgatactc tgaacatctc tcctgcacct tcacagtcac ttgtagttat aattggcgta
     5581 tggacataca caaatccccg ctcctgaaag aactgatgga tggcatatgc aacaagagaa
     5641 cggaccctga ataccgctga ataagcgttt gttctcggcc gtaaatgggc tatggttcgt
     5701 agatactcga aagaatgtct ctttttttgc agaggatagt cctgtgttga aataccttct
     5761 atttcgattc gaatagcctt aatttcaaag ggttgtttgg catttggggt ttctacaatt
     5821 tcaccttcaa ctataatgga agaccctgat gggagtttcg cgatctcatc aaaatttcca
     5881 attgactctc tttcgaatac tacttggaca cctttgaaga aagaaccgtc attcagctca
     5941 ataaaaccga cagcctttga atctctaagt gtacgtatcc agccggaaat tataacagtt
     6001 ttattaacat aatcttcttg gtttctatat atttgcttaa ccgaagccat taacatttta
     6061 agctccattc ctgcagcgtg cgaaatgaat tcgcccttgc aggaacaaaa cttttttatg
     6121 aaaaagtttt tatgggctta aactcattt
//
LOCUS       DAOSDD010000249         6103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4645_length_6103_cov_4.747354, whole genome shotgun sequence.
ACCESSION   DAOSDD010000249 DAOSDD010000000
VERSION     DAOSDD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_6103_cov_4.747354"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK733_16555"
     CDS             complement(<1..455)
                     /locus_tag="PK733_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ12182.1"
                     /translation="MNIIVCVKQVPATNEVKLNKETNTIIREGVEAIINPFDMYAIEE
                     GLRIKENTNGKVTVLSMGIPSVANQLKETVALGVDDTVLLSDRAFAGSDTLATSYALS
                     MGIKKIGEYDLIICGKQATDGDTAQVGPSLAEKLGIPHTTYVRKIEEIK"
     gene            complement(461..1624)
                     /locus_tag="PK733_16560"
     CDS             complement(461..1624)
                     /locus_tag="PK733_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ12183.1"
                     /translation="MNYFLTEEQQMIKELAARIADEKIAPLALKYDEEGIFPRDIVKI
                     LADSDLCGVYIPEEYGGLGGGVFEMSLVVEELSRACGGIALAFAGTGLGSFPILLFGN
                     EEQKQKYLPDIASGKKLAAFGLTEANAGSDAAGIQTTAVLENSEYVLNGTKQWITNGG
                     EADIYTVIACTNRSKGARGFSAFIVEKGTPGFSFGKKENKMGIRASVTSELVFDNCRI
                     PKENLLSREGMGFLVAMKTLDRTRPGVAAQALGIAQGAFDEALKYSRERVQFGQAISS
                     FQAIQHMLADMAIQIEAARCLIYQTCRHIDSGEKNISKESAMSKVMASDVAMKVTVDA
                     VQIMGGYGYMKEYPVEKRMRDAKITQIYEGTNQIQRNVIALELIKGLAQNKMG"
     gene            1932..3293
                     /gene="rimO"
                     /locus_tag="PK733_16565"
     CDS             1932..3293
                     /gene="rimO"
                     /locus_tag="PK733_16565"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ12184.1"
                     /translation="MNNNIQRKKVGLISLGCPKNLVDSEVMLGQLKEEGYEITNNEHE
                     ADIIIVNTCGFINDAKQESINAILEMASLKKQNCELLIVTGCLAERYNELIVKEIPEV
                     DAVLGTGHYGMIAEAIRKAYSGTVEKQVLWGDPGYCNFEGDKRLLSTPKGYSFLKIAD
                     GCNNHCTYCVIPSLRGRYRSRKMEDIIHEAGYLVDSGAKELILIAQDTTKYGIDLYGE
                     RKLKDLICEISKIEKLEWIRLLYCYPDQIDDDLIDEIANNVKVCKYLDVPIQHSSDKI
                     LKNMGRKGSSVDIKNIIAKLREKIPEIYIRTTMIVGFPGEDEEDFANLHEFVKKLEFE
                     RLGVFMYSREEGTPAAKIKSQVKKDIKRKRHQNLMALQNKITVEKNKSRLNKSYDVLV
                     EGVADDGIFYYGRSYAEAPDIDGLIYFTSSYPLEEGSFVKVKILNIEQYDLIGVAYDE
                     FTK"
     gene            3277..3894
                     /gene="pgsA"
                     /locus_tag="PK733_16570"
     CDS             3277..3894
                     /gene="pgsA"
                     /locus_tag="PK733_16570"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOJ12185.1"
                     /translation="MNLPNKITLSRILIVPIFMLFILPIPEVFINSIHWQFLANQLRM
                     LSNFIAHFGHYIAAIIFIIAASTDGIDGYIARKTKQITKFGIFLDPVADKLLVTAALI
                     ALVQRGDISGWSAMIIIGRELMVMGLRLVAAGEGTVISASKLGKAKTITQVIAISATL
                     LQNYPLSLFTDFRFDKYAIIAAVVITIYSGYDYLAKNIKFIRSDN"
     gene            4119..4685
                     /gene="fapR"
                     /locus_tag="PK733_16575"
     CDS             4119..4685
                     /gene="fapR"
                     /locus_tag="PK733_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HOJ12186.1"
                     /translation="MGKSSLLKKERQSILFDKIKSDPFLTDEELADFFSVSVPTIRLD
                     RLELGIPELRERIKNVAEKNHAKVKSLGSREIVGELVEINLGVNAISVLETDESMVFE
                     KSKIVRGHFIYSLAESLAIALIDADVALVGVANVKYKIPVYAGSKLVAKAEVKRVKGN
                     SFIVWVKIMQKQVEVFRGKFILVSLEKL"
     gene            4776..5789
                     /gene="plsX"
                     /locus_tag="PK733_16580"
     CDS             4776..5789
                     /gene="plsX"
                     /locus_tag="PK733_16580"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOJ12187.1"
                     /translation="MKIIVDAMGGDNAPHDIVKGCLDAVKEQEGFNILLLGDGSEIDK
                     ILKENRFSSGRIDVQHTTEVITNDDIPTKSIKVKKDSSMVVGFNMLKENKGDVFISAG
                     NTGALMTGATFILGRIEGIDRPALAPVIPTKKGGALLIDAGFNTVCKPLNYLQFGIMG
                     SVYMREIFKISNPKIGLINVGAEERKGSEIIRQAYNLLSASGLNFIGNIEGREIPEGE
                     VDVAVCDGFVGNVLLKFLEGVGSFIFDGLKDVYYKNFISKLASLLVKGELRKFKKRLD
                     YEEYGGVPVLGINGTVFKCHGNSNAKSFKNALIKAYYFAKTAIMEQIKQEISCVEVRN
                     VGM"
     gene            5776..>6103
                     /locus_tag="PK733_16585"
     CDS             5776..>6103
                     /locus_tag="PK733_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOJ12188.1"
                     /translation="MLECNKIHPSGGILGTGSCVPDKILTNIDLEKMVDTSDEWIVKR
                     TGISERRILNSNTPLYELGVKASRKALEDAGITPEDIDLIIVTTEAPDYLTPSMSCLV
                     QKDLGAI"
BASE COUNT         1999 a          977 c         1209 g         1918 t
ORIGIN      
        1 tcttttattt cttcaatttt ccttacataa gttgtatgag gaattcccaa tttttcggca
       61 agacttggtc caacttgagc agtgtcgcca tcagttgctt gcttgccgca tataattaaa
      121 tcatattctc caattttctt tatacccatt gatagagcat aagaagtggc aagggtatct
      181 gaacctgcaa aagctctgtc gcttaaaaga acagtatcgt ccacacccaa agcaacagtt
      241 tctttcaact ggtttgcaac acttggtata cccatgctca ggacagtaac ttttccgttt
      301 gtattttctt tgattctaag tccttcctcg attgcataca tatcgaaggg attaattatt
      361 gcttctactc cttcacggat tattgtattt gtttctttat tcaatttaac ttcattagtt
      421 gcgggaacct gtttgacgca gactataata ttcaatattt tcaccccatt ttgttttgag
      481 ccagaccctt tataagctcc agagcaatga catttctctg gatctggttt gttccttcat
      541 atatctgagt tattttagca tctctcatcc ttttttcaac cgggtactct ttcatatagc
      601 catacccgcc cataatctgt accgcatcta cagttacctt cattgccaca tcggaagcca
      661 taacctttga cattgcagac tctttggata tatttttttc tccactgtct atatgtctgc
      721 aagtttgata tatcaaacat ctggcagcct caatttgtat agccatatca gcaagcatat
      781 gctgtattgc ttgaaaagat gagattgcct gaccaaactg taccctttcg cgggaatatt
      841 taagagcctc atcaaatgct ccctgagcta tgccaagagc ttgagcggca acaccgggtc
      901 ttgttctgtc aagtgtcttc atggccacaa gaaatcccat gccttctctg gaaagtaaat
      961 tttctttagg aattctacag ttgtcaaaaa ctaactcgct ggtaaccgaa gccctgatac
     1021 ccattttgtt ttctttcttg ccaaaggaaa aaccaggcgt tcctttctcc acgataaaag
     1081 cagagaaccc tcttgcacct ttagatctgt tggtacatgc aataactgta tatatatcag
     1141 cttctccgcc attggtaatc cattgctttg taccattcaa aacatattcg ctattttcaa
     1201 gaactgcagt agtttgtatg cctgcagcat cactacctgc atttgcttca gtcaatccaa
     1261 aagctgcaag ttttttccct gaagcaatat caggaagata tttttgtttt tgctcttcat
     1321 tgccaaaaag caaaatggga aatgagccta gacctgtgcc ggcaaaagcc agtgctatac
     1381 ctccgcatgc tctgcttagt tcctcaacta caagagacat ttcaaataca ccgccgccaa
     1441 ggcctccata ttcttcagga atgtaaaccc cgcaaaggtc cgaatctgca agaattttaa
     1501 caatatcacg gggaaatatg ccttcctcat catatttcaa agccagcggt gctatttttt
     1561 catcggctat tcttgcagcc aattctttga tcatttgttg ctcttctgtt aagaaataat
     1621 tcattaaaat acacccctta aattaaaatt aagtagtaat ttcaggatta aacttgtaca
     1681 tggaatctat aaaatctaat gtcatctaat gtcatctaat tttgtataga ttccatttag
     1741 tatatactat tattagctgg taataattaa ttataaaata aagtataata caatttcatt
     1801 gttaatgcaa gcagggtgtt gtattttgtg cccggcacaa aatacaacac aaaaattaaa
     1861 gtaattgaca attctgtatg ggcaatttaa aataaagtta ctacttatat tggaagtgaa
     1921 ataatttggc actgaataat aacattcaaa gaaaaaaggt aggtttgata tcactgggtt
     1981 gtccgaaaaa tcttgtagat agcgaggtga tgctagggca gcttaaagaa gaaggctatg
     2041 agattactaa taatgagcat gaggcagata taattattgt taatacttgt gggtttatta
     2101 atgatgccaa gcaggaatca ataaatgcaa tattggaaat ggcttctcta aaaaaacaga
     2161 attgtgaact tcttattgta acaggttgtc ttgccgaaag atataacgag ctgattgtaa
     2221 aggagattcc tgaggtagat gcagtgctgg gtacagggca ttatggcatg atcgccgaag
     2281 ctataagaaa agcatactcg gggacggttg aaaagcaggt tttatggggt gatcctgggt
     2341 attgcaattt tgaaggtgat aaaaggcttc tttcaactcc aaagggttat tcattcctca
     2401 aaattgccga cggttgcaac aaccactgta cttactgtgt aatcccctca ttacgaggca
     2461 ggtatagaag ccggaaaatg gaggacatta tacatgaagc aggatatctt gttgattctg
     2521 gtgcaaagga acttattcta attgctcagg acacaacaaa gtatggtata gacctttatg
     2581 gtgaaaggaa acttaaggat cttatttgtg aaatcagtaa aattgagaag ctggaatgga
     2641 tacgtctgct ttattgctac cctgatcaga tagatgatga ccttatagat gaaatagcta
     2701 ataatgttaa agtatgtaaa tatcttgatg ttccaataca acacagcagt gataaaatac
     2761 tgaaaaatat gggacgtaaa gggagttctg ttgacattaa aaatataata gcaaaattaa
     2821 gagaaaagat accggaaatt tatatcagga caaccatgat tgtaggtttt cctggagaag
     2881 atgaagaaga cttcgctaat ttacacgaat ttgttaagaa actggaattt gagagattgg
     2941 gagtgtttat gtactcaaga gaggaaggta ctcctgcagc taagataaaa agtcaggtaa
     3001 aaaaagatat taagagaaaa aggcaccaaa atttaatggc tttgcaaaac aaaataactg
     3061 tagaaaaaaa taaatccagg ctgaacaaaa gttatgatgt tttagtagaa ggtgttgcgg
     3121 atgacggaat attttattat ggacgaagtt atgctgaggc acctgatatt gatgggttaa
     3181 tatattttac aagcagttat ccccttgaag aaggcagttt tgtaaaagta aaaatattaa
     3241 atatagaaca gtacgattta ataggagttg cttacgatga atttaccaaa taaaataact
     3301 ttatcaagaa ttttaatagt acctattttt atgcttttta tactacccat accggaagta
     3361 tttattaaca gtattcattg gcagttttta gctaatcaac ttagaatgtt aagtaatttt
     3421 attgcgcatt ttgggcatta tattgctgct attatattta ttattgcagc cagtacagat
     3481 ggaatagatg gatatatagc aagaaaaaca aagcaaatta ccaaatttgg cattttcctt
     3541 gatcctgtgg cagataagct tttggtaaca gctgcgctta tagctcttgt acagagaggc
     3601 gatatttcag gttggtcggc tatgattatt attggaagag aacttatggt aatgggtctc
     3661 aggctggtgg cagcaggtga aggaacagtt atttctgcaa gcaagcttgg caaggcaaaa
     3721 actataactc aagttatagc catttcagct actttgcttc aaaactatcc cttaagcttg
     3781 tttactgatt tcagatttga taagtatgct ataattgctg cagtagttat tacaatttat
     3841 tctggttatg actatctggc taaaaatata aaatttatca gaagtgataa ttagctggta
     3901 gtagaaaaaa ttttacttgg ctcttttgct ggccataatg ccataacttt tttataggtt
     3961 tttatagcaa atggttgcat attgcaaaaa tttatactat actgttagta acaacaatta
     4021 gtagctggta ataatattaa gttataaccg aagattttct actgatgcgt tataacaact
     4081 gcagcagaaa cttatctgct tgcagggagg tgttttaatt gggaaagtct tctctgctaa
     4141 aaaaagagag acagagcatt ctttttgata aaattaaatc cgatccgttt ttgacagatg
     4201 aagaactagc tgattttttc tctgtaagtg ttcctacaat acgtcttgac agattggaac
     4261 tcggcattcc cgaactgcgt gaacgcataa agaatgtagc ggaaaaaaac catgcaaagg
     4321 ttaaatctct agggagcagg gaaattgttg gtgagctcgt agaaattaat cttggtgtaa
     4381 atgcgatttc agtactggaa acagacgaat ctatggtttt tgaaaaatca aaaattgtcc
     4441 gtggacattt tatatattca ctggcagaat ctctagctat tgctttaata gatgctgatg
     4501 tagctttagt aggtgtagcg aatgtaaaat ataagattcc tgtttatgcg ggaagtaaac
     4561 tggtagcaaa agccgaagtg aaaagagtaa agggaaatag ctttatagtt tgggtaaaaa
     4621 taatgcaaaa acaggttgaa gttttcagag gaaaatttat actggtatcc cttgaaaagt
     4681 tgtaagttaa tcatttaaaa tattattcat gggtttagat catactttgt attaaaggca
     4741 attttaataa atgattattt tagggagtgg atgctttgaa aattatagta gatgctatgg
     4801 gcggtgataa tgcaccccac gatatcgtaa agggctgtct tgatgccgta aaagaacagg
     4861 aaggatttaa tatattgctt ttaggcgatg gcagtgaaat agataaaata ctaaaagaaa
     4921 ataggtttag cagtggtaga atagatgttc aacatacaac tgaagtcatt acaaatgacg
     4981 atatacccac aaaatctatt aaggttaaaa aagattcatc tatggtagtt ggttttaaca
     5041 tgcttaaaga aaataaaggt gatgttttta tttccgccgg taataccgga gcattaatga
     5101 caggggctac atttattctg ggtcgtatag agggaataga cagacctgct ctggcacctg
     5161 taatacctac aaaaaaaggc ggagcacttt taatagatgc agggtttaat actgtctgca
     5221 aaccattgaa ttacctgcag tttggtatta tgggatccgt ttatatgagg gaaatattta
     5281 aaattagcaa ccctaaaata ggtttaataa atgtgggagc tgaagaaaga aaaggcagtg
     5341 aaataattcg tcaggcttat aatcttcttt ctgcatcagg attgaatttc attggtaata
     5401 ttgaaggcag ggaaatacct gaaggcgaag ttgatgtagc agtttgtgat ggctttgttg
     5461 gtaatgtcct tctaaaattc cttgaaggtg taggttcgtt tatatttgat gggttaaagg
     5521 atgtatatta caaaaacttt atatcaaaac ttgcttcgct ccttgttaaa ggagaattaa
     5581 gaaagtttaa aaaacgtctt gattatgaag aatatggagg agtacctgta cttgggatta
     5641 acggcactgt atttaaatgc catggcaatt caaatgcaaa gagtttcaaa aacgctctaa
     5701 taaaagctta ttattttgca aaaactgcga ttatggaaca aattaagcag gaaataagct
     5761 gtgtggaggt taggaatgtt ggaatgtaat aaaattcatc cgtcgggggg gatacttggt
     5821 acaggaagtt gtgtgcctga taaaatatta acaaacattg atctggaaaa aatggttgat
     5881 acttcagatg agtggatagt taaaaggact ggaatttcgg aaagaaggat tttaaatagt
     5941 aatacaccac tttatgaact aggagttaaa gcctctagaa aggcgttgga agatgctggg
     6001 ataacccctg aagatattga tttaataata gtgactacag aagctcctga ttacttaaca
     6061 ccatcaatgt catgcctggt gcaaaaagac cttggagcca taa
//
LOCUS       DAOSDD010000250         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4688_length_6053_cov_5.918640, whole genome shotgun sequence.
ACCESSION   DAOSDD010000250 DAOSDD010000000
VERSION     DAOSDD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_6053_cov_5.918640"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PK733_16590"
     CDS             complement(<1..178)
                     /locus_tag="PK733_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HOJ12189.1"
                     /translation="MNIKNEQTPSRALDSGPFYHGTKADLNIGDLLVPGYSSNYGERK
                     KANFIYFTATMDAAI"
     gene            complement(278..505)
                     /locus_tag="PK733_16595"
     CDS             complement(278..505)
                     /locus_tag="PK733_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12190.1"
                     /translation="MKSLYSYKSIKPSSAGGCETNYRIVEKEIFKLVGFKKTVAIIFQ
                     CTNPEIAKIKKAGWMLKQNNSTTYSSYNVHK"
     gene            complement(561..2480)
                     /locus_tag="PK733_16600"
     CDS             complement(561..2480)
                     /locus_tag="PK733_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ12191.1"
                     /translation="MKTNFKMLPQKVSALLLVFLMVFAFTAPTYAAQTWNKGAKVNYV
                     PVNGDKVYKSSNWAESLAKEYQITWYENPTREVLKGEFMLVQLRTIQASLARRGLPLI
                     FAYNKLDNFKDIGTVTKAAKEEITILNSLGILSGTVDGYMNLGNVIKRSEAAKILDVT
                     NKEVLHIALTRPDKVFKDMKGHWAEKNVSNAYRTTLINGTSETTFSPNTSLTIEQTLQ
                     ILENEVGFYGITRADVAKALKETFKVTTATTFSNSLVTKYSPDMLETKMNIYAFNNIY
                     DNGSAKPDEKVTKVEVLKLAVSSIYNYSNFDSFWFYPEMASDAELFKIMKEKGVVDGS
                     DSLNDQLKYIDVIRYFANAKKAYLRLELSSGDDVEVKNISKYSAEDQKVIKDMVANKI
                     VDAPNNELPLDKPVVKGMVNEIVVNFVERYSTITPNKDKLNTNPDNIPSNYKDYPYTL
                     ASMDKRVYEIPNYLVDDDYKNSVQVFGKLKEDYASIEESVNRYLDIVLNVDYTNFDEA
                     KFKQDIQGIALMPVTDVQFREFFKYVKSNKIQTSGTSKVQFPAIYFDGITYRVRTEVK
                     LNIQNADQPYNLLFLDLLQPGKVKYEGKNITRIIDIPVGRYINSPQFAVYVTALTPIP
                     INLNNDLIVVFKNTF"
     gene            complement(2642..2851)
                     /locus_tag="PK733_16605"
     CDS             complement(2642..2851)
                     /locus_tag="PK733_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12192.1"
                     /translation="MFCSVEFTEGGKSYYYISDDDTIEVGDLVIVPVGKDEHTATVEV
                     VNIEYFSEEKAPFPIEKAKHIIGRG"
     gene            complement(2880..3023)
                     /locus_tag="PK733_16610"
     CDS             complement(2880..3023)
                     /locus_tag="PK733_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12193.1"
                     /translation="MEIWDILDKNGNMTGKTITRGDNLRDGEYHLIVHIWIINGKGET
                     FSI"
     gene            complement(3256..3343)
                     /locus_tag="PK733_16615"
     tRNA            complement(3256..3343)
                     /locus_tag="PK733_16615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3306..3308),aa:Leu,seq:caa)
     gene            3466..3807
                     /locus_tag="PK733_16620"
     CDS             3466..3807
                     /locus_tag="PK733_16620"
                     /inference="COORDINATES: protein motif:HMM:NF037099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12194.1"
                     /translation="MQKKLIKLKRFNLVMGFFHLVQGIIMIFLATNVIQKIGEFKPTI
                     IQFYLRFNPVTKSLEPASKELFELPFGILVASFLLISAAAHSLIYLNSEKYFADLKRG
                     LNKYRLLELIL"
     gene            4407..4631
                     /locus_tag="PK733_16625"
     CDS             4407..4631
                     /locus_tag="PK733_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12195.1"
                     /translation="MFEAIAQRVNVRFHLSGLSPEETKGYIKHHLQVAGGKRELFSDD
                     AVKLIHNFTHGIPRIINNICVGCLLDTMPK"
     gene            complement(4705..5037)
                     /locus_tag="PK733_16630"
     CDS             complement(4705..5037)
                     /locus_tag="PK733_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12196.1"
                     /translation="MQKFFPALESLPHSCTLARLLEDIDVEHIEDVAVNFVKRLIRDK
                     KLVNYMVSKRYLIVIDGVHKFTREWEWCENSLKKHKKGQPEGVYQYYASTLEASLGLH
                     HAFEKRFR"
     gene            complement(5080..5523)
                     /locus_tag="PK733_16635"
     CDS             complement(5080..5523)
                     /locus_tag="PK733_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12197.1"
                     /translation="MKKHIEEINGYIANNPFIRKEPFKHWFMRNSFLLHKKLCITISK
                     WHPLSDWQKARWLNERGFYRRREANAAVNCIDVDNKIAAQPTFDIEFISILELIPKEQ
                     MNDVISGIEKFEKNIVIIVFMIVTLTMPDIFKIFMMGMHIQVSGT"
     gene            5734..5958
                     /locus_tag="PK733_16640"
     CDS             5734..5958
                     /locus_tag="PK733_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ12198.1"
                     /translation="MATSYNKLWKLLIDKKMSKADLRKASGVSPNTMTKLRRDEPVML
                     SVLDKICKVLDVNYGDIVDYVADEERSDKS"
BASE COUNT         1847 a         1114 c          948 g         2144 t
ORIGIN      
        1 atatagctgc atccatggtt gcggtaaaat aaatgaagtt tgccttcttt cgctcaccat
       61 agttagagct atagccaggt accagcaagt ctcctatatt caagtcggct tttgtgccgt
      121 ggtagaaagg ccctgagtcc aacgctctgg atggtgtctg ttcgttcttt atattcactt
      181 tttatccctt cctttcgtga gtaacaggct ttaccattac ctgttactcg agttgataaa
      241 agcttgctta cacttttaag taagccagtt ttgaccatta tttatgcaca ttataagaag
      301 aatacgtagt gctattattt tgtttcaaca tccaacctgc tttcttaatc tttgcaatct
      361 ctggattggt gcattgaaaa ataattgcaa ctgtcttctt aaaacctact aatttaaata
      421 tttctttctc aacaatacga taattcgttt cgcatcctcc tgcagatgat ggtttaatac
      481 ttttataaga gtacaagctt ttcaaagcct gtactcatta tacttgatat taaagtcaaa
      541 acgcttagtt aagcaatatt ctaaaaagtg tttttgaata ctactattaa atcattgttt
      601 aagttaattg gaattggtgt taatgcagta acatatacag caaactgtgg actgtttata
      661 tatctaccta ctggtatatc tattatacga gttatatttt taccctcgta tttaaccttg
      721 cctggttgca acaaatccaa gaataataga ttgtatggct ggtctgcatt ttgtatattt
      781 agtttaacct cagttcttac tctgtacgta attccatcaa aatatatcgc tggaaattgt
      841 acttttgatg tcccagatgt ttgtatttta ttgctcttaa catatttaaa aaattctcta
      901 aattgaacat ctgttactgg catcagcgct ataccttgga tatcttgttt aaattttgct
      961 tcgtcaaaat tcgtatagtc tacatttaaa actatatcca aatacctgtt cacactttct
     1021 tcaatacttg catagtcttc ttttaattta ccaaatacct gaactgaatt tttatagtca
     1081 tcatctacaa gataatttgg tatttcataa actcttttat ccatagatgc cagagtgtat
     1141 gggtaatctt tgtaattaga tggtatgtta tctggatttg tgtttaactt gtctttgtta
     1201 ggtgttatag tactatacct ttcaacgaag ttaacaacta tctcatttac cataccttta
     1261 actacaggtt tatccaaagg taactcattg tttggtgcat ctactatttt gttagcaacc
     1321 atatctttaa ttaccttttg gtcctctgct gaatacttgg aaatgttttt tacttccaca
     1381 tcatctccac tgcttagttc caatctcaag tatgcttttt tagcatttgc aaaatacctt
     1441 atcacatcaa tatatttgag ctgatcatta agactatctg aaccgtctac aacacctttt
     1501 tctttcataa tcttaaagag ttctgcatct gaagccattt cagggtaaaa ccagaaacta
     1561 tcaaaattag aatagttgta gatgctacta accgccaact ttagaacttc cacttttgtc
     1621 actttttcat caggttttgc actaccatta tcataaatgt tgttaaaagc ataaatattc
     1681 atctttgttt caagcatatc aggtgaatat ttagtaacta aactatttga aaaagtagta
     1741 gcagtagtaa ctttaaatgt ttctttaagt gctttagcaa catctgccct tgttattccg
     1801 taaaagccca cttcattttc aagaatttgt aatgtttgct caattgtaag tgaagtgtta
     1861 ggactgaatg ttgtttccga tgtgccgtta attagagttg tacggtatgc attactaaca
     1921 tttttttcag cccaatggcc tttcatatct ttaaaaactt tgtccggcct tgtaagtgct
     1981 atatgcaaaa cttctttgtt agtaacatcc agtatcttag cagcttctga ccttttaatt
     2041 acattgccta ggttcatgta tccatctaca gttccactga gtattcccaa ggaattgagg
     2101 attgttattt cctcttttgc tgcttttgta acagtgccga tatctttaaa attatctagc
     2161 ttgttatatg caaatataag aggtaatccc cgtcttgcta atgatgcttg gattgtacga
     2221 agttgtacta gcatgaattc gccctttaaa acttcgcgtg taggattttc ataccatgta
     2281 atctgatatt cctttgctaa actttccgcc cagttacttg atttgtacac tttatcccca
     2341 ttaacaggta cataattaac ttttgcacct ttgttccatg tttgtgctgc ataggtaggt
     2401 gctgtaaacg caaataccat aagaaacaca agtagcaaag ctgacacctt ttggggaagc
     2461 attttgaaat ttgttttcat gaaaattcct cctaattaat attgggtagt gaaacaaaaa
     2521 cacccacccg ttttttattt caaaccctat aatatcaagg gttatataac tttttctctt
     2581 acaagagaca cacagcattc tatgtggctc gtttggtagt acggtcaaca tctatgtcca
     2641 ttcatcctct cccaatgata tgctttgcct tttcaatcgg gaacggtgct ttctcctcag
     2701 aaaaatattc aatatttaca acttctaccg tagcagtatg ttcgtccttt ccgacgggta
     2761 ctattaccaa atctccaact tctattgtat catcatcgct gatgtaataa tagctcttac
     2821 cgccttcagt aaactccaca ctgcaaaaca tatactcacc agctcggtgt ttggctttac
     2881 tatatactga atgtttctcc tttgccatta ataatccata tatgcactat taaatggtat
     2941 tcgccatctc ttaagttatc ccctctagta atagtttttc cagtcatatt cccattctta
     3001 tctaatatat cccatatttc catagaaatt cctccccatt ccacgataaa tactcaattt
     3061 aatttggttg cataatcttc caaatcttaa ctatattata tataaacaat agctaaaacc
     3121 tgcacacccg tgttcataat ttagcaatac tgtgtacaaa tgtataaaat ttcatcttaa
     3181 atttatcccc aaaaacaaca aaactccttg aaatcaagga gtttctatcg catcaatatt
     3241 taaattcttt tatggtggag gcggtggccg ggctcgaacc agcacggtat tgccaccggt
     3301 agattttgag tctactacgt ctgccaattc catcatacca gacatatggt acaaaagata
     3361 aattatcata aaaaattaaa tgttttaagt aacactctcc gcggtataat tgaaacgaat
     3421 tatacacttt aacttataat ttaaaaaata taagggggaa atataatgca aaaaaagtta
     3481 ataaaattaa agcgcttcaa tctggtaatg ggtttttttc atcttgttca gggcattatt
     3541 atgatatttc tagcaacaaa tgttattcaa aagataggtg agtttaagcc aactataatc
     3601 cagttttatc taagatttaa tcctgtaaca aaatctcttg aacctgctag taaggaatta
     3661 tttgaattac ctttcggaat tcttgttgct tctttcttat taatttctgc tgctgctcac
     3721 agtttaattt acttaaatag tgaaaagtat tttgcagatc ttaaaagagg acttaataag
     3781 tatagattgt tggaacttat tttgtagctt ttaacacatt cccgatcaat atgattctac
     3841 aatataaagg tttaggcaag tggaaagatt atcttaatgg agaaagaata tatatataat
     3901 acttagtctt gctgctaaat ccatattggc atggcttgta ctctttggtg ctatgcaacc
     3961 ttaaattgga aaaataatgg gatatatgtt tatagaaggg atatatttta tgagaaaatt
     4021 gataaaaaga atctttatag ttttactttc tattttctta ataataagca taatccccta
     4081 tttagtgcct gtacaatcag cccgggtttt cagctataat aaggaattct tggaaagtga
     4141 atatgcagat attgtatgac ttggtatttg aaaatatctg ggaatccagt atcttacacc
     4201 ttacaattat ttttcagatc tgaatgcaag gattgccaag acctttacat tataaacaga
     4261 tattaatgga ataagtatga ccttttttaa tgaaattact tctttttcat tatcaataag
     4321 cgttttatgt gctgatcggc agttcaaaaa atgtatttaa ttcagatatc aaatgttttt
     4381 aaacatcccc cggataaagg cttaaaatgt ttgaagccat agcccaaagg gtaaatgtaa
     4441 gatttcatct atcgggatta tctccagaag aaacaaaggg atatatcaaa catcatttac
     4501 aggttgcggg tggtaaacgg gaactatttt cagatgatgc ggttaaactt attcataact
     4561 tcactcacgg cataccaaga ataatcaata atatatgtgt aggatgtctg ctggatacca
     4621 tgccaaagtg aaattgtaga cccaagcagc gctgagaggg tttatcaaga aatgtagcag
     4681 gtgtaatcac taaacagaaa ggagtcatct aaaacgcttt tcgaatgcgt gatgcaaacc
     4741 cagcgatgcc tccaatgtgc ttgcataata ttggtataca ccttcaggct ggcctttttt
     4801 gtgtttttta agactgtttt cacaccattc ccattccctg gtaaatttat gcaccccatc
     4861 aatcactatg agataccgct tactgaccat ataatttaca agctttttat cccttatcag
     4921 cctttttaca aaatttactg caacatcttc aatatgttct acatcaatgt cttccagcag
     4981 acgagccaag gtacaggagt gaggaagact ttcaagtgca gggaaaaatt tctgcatatt
     5041 ctcaatgata tttattcaat ttacttttat ctttaatact taagttcccg agacttgaat
     5101 atgcatcccc atcataaaaa ttttgaaaat atccggcatc gttaacgtga caatcataaa
     5161 tactattatc actatgtttt tttcaaattt ttctattcct gatattacat cattcatttg
     5221 ttcttttggg attagttcca gtatcgatat aaactcgata tcaaaggtag gttgagcggc
     5281 tattttatta tcaacatcta tacagttaac agcagcattt gcttctcttc gcctataaaa
     5341 acctctttca tttaaccacc ttgccttctg ccaatcggat aaggggtgcc attttgaaat
     5401 cgtaatgcat aactttttgt gtaataaaaa tgaatttcgc ataaaccaat gcttgaatgg
     5461 ttcttttctt atgaatggat tgtttgcaat atagccattg atttcttcta tatgtttttt
     5521 cataacagtg ttaactttga aaattactct tgtaattcac catacttcat ttctgttatt
     5581 actcccctag catccactcc actatcgaac ctctgaatag taacgagtcc aatttatatt
     5641 catctcataa tttgcgaaac tgtattgtat tttgagagag tatgtactat tatatatggt
     5701 agaatattgt atacacaagg ctggaggcgt attatggcta catcatacaa taaactgtgg
     5761 aaactgctca tagataaaaa aatgagcaaa gccgacttgc gaaaagcgtc cggtgtgtct
     5821 ccgaacacga tgacaaagct gcgccgagac gaaccggtga tgctgagcgt gttggacaaa
     5881 atatgtaagg tgctggatgt caactatggg gacatcgttg attatgtggc agatgaggaa
     5941 aggagcgata aatcgtgatt ggtaaaaagc aaatgaccga ggaagaaata aagctgaatt
     6001 ttatcactcc tgttattacc gacaagtggt caggttagat gttttttgat tac
//
LOCUS       DAOSDD010000251         6037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4701_length_6037_cov_4.903377, whole genome shotgun sequence.
ACCESSION   DAOSDD010000251 DAOSDD010000000
VERSION     DAOSDD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4701_length_6037_cov_4.903377"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PK733_16645"
     CDS             <1..756
                     /locus_tag="PK733_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ12199.1"
                     /translation="GYIDSKNPFDSWITCSYSIAFWLSNRIFQLPRYNLGLTCGLCGT
                     GFCVDLGVLKKIGWGATCLTEDLEFTMKLALNDIKVAWAHNAVVYDEKPITLKQSWKQ
                     RKRWMQGHADCASRFLIPLFKKAVKDSSLVAFDCALYLFQPLRFIFLGFATVLMWVQT
                     IYPESPFYNLKYVFPVILWQIYVLFQFFYGPLIIISEKKFNPIVILGFIVYPIYCLTW
                     VPITIQGFLSKNNKNWNHTTHTREISITELEKA"
     gene            complement(877..3315)
                     /locus_tag="PK733_16650"
     CDS             complement(877..3315)
                     /locus_tag="PK733_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOJ12200.1"
                     /translation="MYGRFTERAEKALAYSQQLAVELGHNYVGTEHLLLGLVKEGSGV
                     AARVLQSQGVTEEKIIRQIDELIGRSEKTGEQPLGFTPRTKRVLELSFREARKMNQNY
                     IGTEHLLLGIMREGESVAVRILIDLGVDPQKMMNEIAKMLNEEAPGAPSAQKNNSGYT
                     NTPTLNQFGRDLTDMAREGKFDPIIGRDKEIERVVQILCRRTKNNPCLIGEPGVGKTA
                     IAEGLAQKIVEGNIPEILKDKRVVTLDLSAMVAGAKYRGEFEERLKKAMDEIRKAGNV
                     ILFIDEMHTIIGAGAAEGAIDASNILKPSLARGEIQVIGATTLDEYRKHVEKDAALER
                     RFQPITVGEPSKEEALDILKGIRDKYEAHHRVKITDDAIEAAVRLSDRYITDRYLPDK
                     AIDLIDEAASRVRLKAFTAPPDLKEMEEQLEKLIKEKEDAIRCQEFEKAAKIRDKEQE
                     LKQRLAKGKSNWEQKNQTQTDRVTEEEIAEIVASWTGIPVKRLAEEETERLMKMEDIL
                     HKRVIGQDEAVKAISKAIRRGRVGLKDPKRPVGSFIFLGPTGVGKTELSKALAEALFG
                     DENSIVRIDMSEYMERFAVSRLVGSPPGYVGYDEGGQLTEKVRRKPYSVVLFDEIEKA
                     HPDVFNILLQILEDGRLTDSQGRVVDFRNTVIIMTSNVGARLITEPKRLGFTAGEDNK
                     AQNYEDMKSNVMGELKKTFRPEFLNRIDEIIVFHPLYEEHIKQIVSLMIDKLAGRLKQ
                     NGISIEVTDNAKTLLAQKGYDPVFGARPLRRSIQGMIEDRLAEEILEGKVNTGDTVLV
                     DKDPEEDKIVFNTK"
     gene            complement(3410..4432)
                     /locus_tag="PK733_16655"
     CDS             complement(3410..4432)
                     /locus_tag="PK733_16655"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOJ12201.1"
                     /translation="MEANNENNGVIISSRVRLARNFEEYPFPYRMNKAQADEIISKVR
                     SSIAAGGLNELKGSLFVDMQNFGEIERQMLVEKHLISPDLTMSCEKSAVVISRDEKVS
                     IMINEEDHLRIQAIYSGLKLNDALILCNKIDDCLEKNINYAYSSTYGYLTCCPTNIGT
                     GIRASVMLHLPALVMTGYIRNVLEACSKLNMTVRGIYGEHSGALGNIFQMSNQVSLGQ
                     TEEEIVTSITNIATQIVEQENTIRNELYKQNAKLFEDKIYRSFGLLSNARIITTEESL
                     NLISDVKLGVDMGIIKDVNINTVNEIILLIQPASLQKFVGKPISPDERDIKRAELIRE
                     KLKASQ"
     gene            complement(4416..4949)
                     /locus_tag="PK733_16660"
     CDS             complement(4416..4949)
                     /locus_tag="PK733_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOJ12202.1"
                     /translation="MQCQLCQKRQATVHFTKILNNQKVEMHLCEQCANEKKQQFGFGV
                     PISINALIAGLLGFDSNDSNQIGSYIQPFQKVLKCEKCGMTIEEFQKTGKLGCSNCYT
                     TFDEKLSPLIRRLQGSVEHNGKVPGRLSNNMSVTKEITKLKELLNRAVEREEYEKAAE
                     IRDQIKSLEVGLNGSKQ"
     gene            complement(4965..5423)
                     /locus_tag="PK733_16665"
     CDS             complement(4965..5423)
                     /locus_tag="PK733_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOJ12203.1"
                     /translation="MAILSDIIEQFIKKMIEEMDGVIEIQRNELASHFNCVPSQINYV
                     IDTRFTSEKGYYVESRRGGGGHIKIKRVNFSEPTSYLMHIINSMGYSITQQSAEVFII
                     NFEDYNIITDRDALLMKAATSDKVLSSIPTGERDNIRAAILKNMLISLIV"
     gene            complement(5627..>6037)
                     /gene="fusA"
                     /locus_tag="PK733_16670"
     CDS             complement(5627..>6037)
                     /gene="fusA"
                     /locus_tag="PK733_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621539.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ12204.1"
                     /translation="VDGSFHPVDSSEMAFKIAASLAYKKGMAEASPVLLEPIMHVEVY
                     VPDSYMGDIIGDLNKRRGRILGMNPQHDGTQQVVAEVPQAEMFKYATDLRSMTQGRGY
                     FKEWYERYEEVPPMISQKIIDEAKKTMTDDDNND"
BASE COUNT         1551 a         1376 c          973 g         2137 t
ORIGIN      
        1 ggatatattg atagtaaaaa tccatttgat tcatggatta cctgttccta ttcgatagct
       61 ttctggcttt caaatagaat atttcaactt ccaagataca atttgggtct tacctgtggt
      121 ctatgcggta caggattttg tgtagatctg ggagtactta agaaaatcgg ctggggagcc
      181 acatgcctta cagaagatct tgaatttaca atgaagctgg cattaaatga tataaaggta
      241 gcatgggccc ataacgcagt agtctatgat gaaaagccta taaccctcaa gcaatcctgg
      301 aaacagcgca agcgctggat gcagggtcat gccgactgtg catcacggtt tcttataccg
      361 ctgtttaaaa aggcagttaa agattccagt cttgtagctt ttgattgcgc cctgtatctg
      421 tttcaacctt tgaggtttat atttttaggc tttgcaactg tattgatgtg ggtacaaaca
      481 atatatccag agtcgccttt ttataattta aagtatgtat tcccggtaat actctggcaa
      541 atatatgttt tatttcaatt tttttatggg ccactcataa taatatctga aaagaagttt
      601 aaccctattg ttatattggg ttttattgta tatcccatat actgtttaac atgggtccct
      661 attaccattc agggtttttt aagcaaaaat aataaaaact ggaatcatac tactcataca
      721 agagaaatca gcattaccga gctggagaaa gcatagaggt gagtgcaggt agatattaat
      781 gggtgcattt ttaatttacg aagaaatata atatgcgccc tgcttcccgc ttctcacttc
      841 tatggcctgt ctctacctat atttgacctt gttttcctat ttcgtgttaa aaacaatttt
      901 atcctcttcc ggatctttat ctacaaggac agtatctcct gtattaactt ttccttccag
      961 tatttcttct gcaagccggt cttcaatcat gccttgaata gatctcctca aaggtcttgc
     1021 tccaaaaaca gggtcataac ctttctgtgc aagaagagtc tttgcattat cagttacctc
     1081 aatacttatt ccattttgtt ttaaccttcc ggccagtttg tctatcataa gacttacaat
     1141 ctgctttata tgctcttcat aaagaggatg gaatacaatt atttcatcta ttctgttcag
     1201 gaattccggt ctgaatgtct ttttcaactc tcccattaca ttgcttttca tatcctcata
     1261 attttgagct ttattatctt ctccggcagt aaagcccagg cgtttcggct cggtaatcaa
     1321 cctggcacct acattggatg tcattataat aactgtgttg cgaaaatcca ccactctgcc
     1381 ctgagaatcg gtaagtctgc catcttcaag aatttgcagg agtatgttaa atacatcagg
     1441 gtgtgctttt tctatttcat caaaaagtac aacagaataa ggctttcttc ttactttttc
     1501 agtgagctga ccaccttcat cataacccac ataacccgga ggtgaaccca ccagccttga
     1561 tacagcaaaa cgctccatat attcggacat gtcaattctt actatagaat tttcatcacc
     1621 aaacaaagcc tcagcaagag ctttactaag ctcggttttc ccgactcctg tagggccaag
     1681 gaaaataaag gaacccacag gacgtttagg atctttcaat cctaccctgc cccttctgat
     1741 agcttttgaa atagctttga ctgcttcatc ctgcccaata acccttttat gaagaatatc
     1801 ctccattttc ataagccttt cggtttcttc ctcagccagc ctttttaccg gaataccagt
     1861 ccaactggct actatctcgg caatttcttc ctctgtgact ctatcggtct gggtttgatt
     1921 tttttgttcc caattgcttt ttccctttgc aagtctctgc tttagttcct gttccttatc
     1981 tcttattttt gcagcttttt caaactcctg acaccttatg gcatcttcct tttctttaat
     2041 gagcttctca agttgctcct ccatttcttt caggtccggg ggagctgtaa aagctttcag
     2101 tcttactctt gaggcagctt catcaattaa gtctattgcc ttgtcaggca gatatctatc
     2161 ggtaatatag cggtctgata gtcttacagc tgcttcaatg gcatcatccg ttatcttcac
     2221 cctgtgatgg gcttcatatt tgtcccttat gccttttaat atatcaagag cttcttcttt
     2281 tgaaggttct cctacggtaa taggctgaaa acgtctttcc agagctgcat ctttttctac
     2341 atgctttcta tattcatcta gagttgtagc accaataacc tgaatttcac ctcttgcaag
     2401 cgaaggttta aggatatttg aagcatcaat tgccccctct gcagctccag caccgattat
     2461 tgtatgcatt tcatctatga acaggatgac attccctgcc tttcgtattt catccatggc
     2521 ctttttcaat ctttcttcaa actcacctct atattttgca cctgcaacca ttgctgaaag
     2581 atccagagta acaacccttt tatctttaag tatctcggga atatttcctt ctactatttt
     2641 ctgagcaagt ccttctgcaa tagcagtttt accaacccca ggctctccta tcagacatgg
     2701 gttgtttttg gttcttctgc aaagtatctg aacaactctt tcaatctcct tgtccctgcc
     2761 gataatgggg tcaaacttgc cttctctggc catatcagtc agatcccggc caaactggtt
     2821 aagtgtaggt gtgtttgtat acccggaatt gttcttttgg gcacttggag caccaggagc
     2881 ttcttcatta agcatttttg caatttcatt catcattttc tgagggtcaa cccccagatc
     2941 tataagaatt ctgacagcaa cactttctcc ttctctcatt attccaagaa gcagatgttc
     3001 tgtgccaata taattctgat tcattttcct ggcttccctg aagctgagct ccaaaactct
     3061 ctttgtcctg ggagtaaacc ccagaggctg ttcaccagtt ttctcacttc ttcctattaa
     3121 ttcatctatt tgtcttatta tcttctcttc tgtaacaccc tggctctgca gaacacgggc
     3181 tgctaccccg cttccttcct taacaagacc taacagcaaa tgctctgtcc caacatagtt
     3241 atgtcccaat tctacagcta attgttggga ataggccagt gctttttcag ctctttcagt
     3301 aaaacgtccg tacatttgtc atcgctcctc tcctttttag tgctgttgta ttatgtgcct
     3361 gttgtatttt gtgccaggca caaaatacaa caggcacata accaaacaat tattgacttg
     3421 cttttagttt ctctcttatt aattctgctc tttttatatc cctttcatcc ggactgattg
     3481 gctttcctac aaatttttga agagatgcag gttgtattaa aagtattatt tcatttactg
     3541 tgtttatatt tacatcttta attattccca tatctacgcc cagcttcaca tccgaaataa
     3601 gattcagact ttcttctgta gtaataatcc tcgcatttga aagcaaacca aaagaacggt
     3661 atattttatc ttcaaatagc tttgcatttt gcttatacag ttcatttctt atggtgtttt
     3721 cctgctcaac aatttgtgtg gcaatattag ttatgcttgt tactatttcc tcttcagtct
     3781 gtcctagaga tacctggttg gacatctgaa atatatttcc caaagccccg ctatgttcgc
     3841 catatatacc tcttacagtc atatttagtt tgctgcatgc ttcaaggaca tttctaatat
     3901 atcctgtcat gaccagtgcc ggcaggtgaa gcatgacaga tgctcttata cctgtcccta
     3961 tattggtggg acagcatgta agataaccat aggtactgct gtatgcatag tttatgttct
     4021 tttcaagaca atcatcaatt ttgttacaaa gtatcagcgc atcatttaac ttaagcccgg
     4081 aataaatggc ctgaatcctc aaatggtctt cttcgttaat cattatacta actttttcgt
     4141 ctctgcttat aacaactgcg cttttctcgc aactcattgt aagatcaggg cttatgagat
     4201 gcttttctac cagcatctgt ctttcaattt ctccaaaatt ctgcatatca acaaatagag
     4261 aacctttcag ctcatttaat ccgccggctg ctatagaact tctaactttg cttattattt
     4321 catcagcctg ggctttattc attctataag gaaatgggta ctcttcaaaa tttcttgcaa
     4381 gccgtactct gctacttata ataactccat tattttcatt gtttgcttcc attaagaccc
     4441 acctccaggc tcttgatctg atctcttatt tcagctgctt tttcatattc ttctctttca
     4501 accgccctgt ttaaaagctc ctttagtttt gtaatctctt tagtaactga catgttattt
     4561 gacaatctgc cgggtacctt cccattatgc tctacgcttc cctgcaatcg tcttattaag
     4621 ggactgagct tttcatcaaa agtagtataa cagttgctgc accccagctt gcctgtcttt
     4681 tggaactctt caattgtcat accacatttt tcgcatttca gcaccttttg aaaaggttgt
     4741 atataagaac caatttggtt ggagtcgttg ctatcaaagc ccaataggcc tgcgattaaa
     4801 gcatttatac ttataggaac tccaaaacca aattgttgtt ttttctcatt tgcacactgc
     4861 tcacaaagat gcatttccac cttctgattg tttaatattt tagtaaaatg tactgttgcc
     4921 tgccttttct ggcataattg gcactgcata aatatcactt ccttttaaac gataaggctg
     4981 ataagcatat tctttaaaat agccgccctt atattgtctc tttcacctgt gggtatacta
     5041 ctcagcacct tgtcacttgt tgctgctttc ataagcagtg cgtctctatc ggtaataata
     5101 ttataatctt caaagtttat aatgaatacc tcggccgact gctgtgtaat gctataaccc
     5161 atggaattga tgatatgcat tagataggag gtaggttccg agaagttaac cctcttgatt
     5221 tttatatgcc cgcccccacc tctcctgctt tcaacatagt accccttctc actggtaaac
     5281 ctggtatcta ttacatagtt aatttgagaa ggtacacagt taaaatggct ggcaagctca
     5341 tttctttgaa tttcaataac cccatccatt tcctcaatca tctttttaat aaactgttct
     5401 ataatatcac ttagtatagc cataagcacc accatattga ttttgacttt ctttgacttt
     5461 cgtttatatt ataaatgatt tatttattat atgcaagttt tttttgaata aacaaaatgc
     5521 gttcttgaat aaatgggaaa aaaagggcac ctgtttacat gttaatgtat tccggcgccc
     5581 tgtttcttct actcactatc cactactcgc tactcacttc tcacttctaa tcattattat
     5641 catcatcggt cattgtcttc tttgcttcat caattatctt ctggcttatc ataggaggta
     5701 cttcttcgta tctttcatac cattctttaa agtatcccct gccttgtgtc atggacctta
     5761 aatcggtagc atatttgaac atttcagcct gaggcacttc tgctactact tgctgagtcc
     5821 catcgtgttg aggattcata ccaagaattc ttcctctgcg tttgtttaaa tctccaataa
     5881 tatctcccat ataactatcg ggcacatata cttcaacgtg cattataggt tccaataaaa
     5941 ctggggaagc ttcagccata cctttcttgt aagccaggct tgcggcaatc ttaaaggcca
     6001 tttcggaaga gtctacaggg tggaaagagc catccac
//
LOCUS       DAOSDD010000252         6021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4720_length_6021_cov_5.224103, whole genome shotgun sequence.
ACCESSION   DAOSDD010000252 DAOSDD010000000
VERSION     DAOSDD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_6021_cov_5.224103"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="PK733_16675"
     CDS             <1..1423
                     /locus_tag="PK733_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12205.1"
                     /translation="NEKGDLRRFVKKITVRQGLLAIPAVIILLLFAPGMFRVFLGEKW
                     YEAGIYAQIFAPSVFMHFIVSPISYIPIIVNRQKTAFIIGLASNLLDIAAVVSGSYIG
                     NDVKTGFYILTFVRLVTLILTLIWYYKISASIKGGTEAKSKSSKDKADIKVKLPKDEL
                     EIETNKIKYKMKIIIIGLPYFACRLAKSLSHFDRDNSYVAIDMNRGILSKLKYIFHLI
                     NADIVYSIGGFVVYSRSAAMAFLLKKKFIMEWVGSDVLNARRAYKENLVDMKIIQQSI
                     HFCEAPWIQKDLAQLGIKTEILYFFTFNTEERQIYKLPDKFSILSYVNESRQEFYGID
                     NLIRLARDFPKIEVRIAGMKTTQKELPQNIKLLGWVKDMGEFYKGCVLFLRLTEHDGL
                     AFSVREALGVGRYVGYSYKYDKTFFVDSYSSLKSLVLELCRSFYNNSLVFNYNGMDFI
                     AKTFSEKKVLGNLVNKFKKIIEN"
     gene            1451..2830
                     /locus_tag="PK733_16680"
     CDS             1451..2830
                     /locus_tag="PK733_16680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ12206.1"
                     /translation="MKKVLVISYYYPPRPGVGTNRIRGLTKYLHRFGWEPVVLTTELG
                     EGEWAPYAKVIETEFCSRIGIIKKFFGFKDGIGLQQQIGINKKRKNGIVNRFFSYLKD
                     FIAFPDEFCTWIKKAEKIGMQLIDNEKISVIISSSSPVSTHLIASRLSRKKGVPWVAD
                     LRDLWTQNHYHDHTLIRRIFERNLEKRTLRYASALVTVSEPYAEQLRLLHGNKDVYCI
                     TNGFDPDEVSDYCPAEKTVSEITPEDIVIEDTVTEDIVTADTAVGDTIEGRLLISHTG
                     SLYDLKKNPGPLFKVIRKLIDNGYMDRSKIQVNFYGPFCHKLEELIKVYKLERIAVQH
                     GIINRNLSMEVQRKSSVLLVMEWDNPGEEGVYTGKIFEYLAARRPILAIGVRGGVLRD
                     LLNTTRAGVYATSEIQIEEFIKRWYEECINGTQPVFIGDKDKINEYSHVNMAGKYSEL
                     LNRVSSSID"
     gene            2936..4348
                     /locus_tag="PK733_16685"
     CDS             2936..4348
                     /locus_tag="PK733_16685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOJ12207.1"
                     /translation="MNLNKLKYKFNRINSDPLLEILLVLIFAVTTNTVIYLNLVLHSI
                     LAISAIAALFFIFADYSNGLVLMVFLIPLDSASVIIPGLTLTKVIGIVTVISFLISNI
                     KHGIKLPDISLTAFLLYAAASIAWSIDIKESFNRITNLANYYVMVIMIINSVNPITSI
                     NSIASINSISTNKNLIKYMNAYLCGSIVVSCLGIYNFITSGGSQRVAAFYGENMNFYG
                     LTIGIAIILSLYLFKNYVNSGNKILKGIYLAACLVLILGQLVSLSRTAWVATCISSFV
                     FFTSSKFNIKRVIISIITLVVIVSVPLLILKRIVPVSTDLFLNRFTTIFTLSDNAAGR
                     LDIWKVAFNIFKDYLFFGVGISNFPAAFTYGYIMNSSAPLDVGLNSDPHNLYISISVE
                     LGITGMLIFLFTIYNIFRNIFSLKENGYKEFCMVLMLFLLVAGLTSTILYKKYFWYVL
                     SLVYAIINQINESEKAKGKY"
     gene            4351..5805
                     /locus_tag="PK733_16690"
     CDS             4351..5805
                     /locus_tag="PK733_16690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ12208.1"
                     /translation="MEKKRLRLIVISHMFPNSYEINGGIFIYEQVKSLAKLSKYNITV
                     ISPIPWSPHMLWFKEKWRKYGQIEEKVIMNKSGNVDGNFNGNMDVNTDDHIDSNIYGN
                     TDGNGNKDDNIDVIYPRYICFPGKHLFFIRAITMFISVAKVIRTLMKRDDIAIIHTHT
                     ILPDGFSGALLKKFFPNIKTVCSARGSDINVYPYRNKMAMYFTRYSLRNNDIITTVSK
                     DLMRKCLELRKKTNKRLNNRPGSELNCNSRSTVNPEVNLAANSKVGSAMNPTANCVVV
                     YNGLKEDWFHLCGEEQIKIEELKKEYGFKTRLLFVGELSKEKGIYELILAFERLQKRY
                     KKIGLLIVGDGRERKEFTNMCSQKDLQDKVIFFGQVKHENVKLLMRSCDIFILPSYSE
                     GMPNVVLEVMAAGKVVIATSVGGVTEVVEHKYNGIVIKPKSVEDIVNSVELLLNDNAL
                     YNSICQNAYTTVRNKFSWNKNALQIDGIYESLLN"
BASE COUNT         2084 a          814 c         1245 g         1878 t
ORIGIN      
        1 taatgaaaaa ggcgacctca ggaggtttgt taaaaagata acagtaagac agggcttact
       61 tgcaataccg gcagttatta ttttactttt gtttgctccg ggaatgttca gggtattttt
      121 aggagaaaaa tggtatgaag ccggtatata tgcacagatt tttgctccca gtgtcttcat
      181 gcattttatt gtatctccca tatcatatat accaataata gttaaccggc agaaaacggc
      241 ttttattata gggctggcta gtaatctgct tgatattgca gcagtggtgt caggcagtta
      301 tataggaaat gatgttaaaa cgggtttcta tattcttaca tttgtaaggt tggtaacgct
      361 tattctaact ctaatttggt attacaaaat atctgcatca atcaaaggtg ggactgaagc
      421 aaaaagtaaa tcatccaaag ataaggctga tataaaagtt aaattaccca aagatgagtt
      481 ggaaatagaa acaaataaga taaaatataa aatgaaaatt attataatag ggcttccata
      541 ttttgcttgc aggctggcaa aaagcctctc tcactttgac agggacaaca gttatgttgc
      601 cattgatatg aatagaggta ttttaagtaa gttaaaatat atttttcatc tgataaatgc
      661 agatattgta tactctatag gaggtttcgt cgtatatagc agatctgcag caatggcttt
      721 tctactcaaa aagaagttta tcatggaatg ggtgggttcg gatgttttga atgcaaggag
      781 agcatacaaa gagaatcttg ttgacatgaa aattatccaa cagtcaattc atttttgcga
      841 agctccatgg attcaaaaag atttggctca attaggtata aagactgaaa tattatactt
      901 ttttacattt aataccgagg aaagacagat atacaagctg cctgataaat tttcaattct
      961 ttcctatgta aatgaaagca ggcaggagtt ttatggtata gataatttaa ttagacttgc
     1021 tcgtgatttc cccaaaattg aagtaagaat agcaggaatg aaaactaccc aaaaagagct
     1081 tcctcaaaac attaaattat taggatgggt aaaagacatg ggggagtttt ataaaggctg
     1141 cgttttattt ttaagactta cagaacacga cggactggct ttttctgtga gagaagcact
     1201 gggagtggga aggtatgtgg gttattccta taaatatgat aagacatttt ttgtagatag
     1261 ctactctagt ttaaaatctc ttgttttaga attatgcaga agcttttaca acaattcgct
     1321 ggtgtttaac tataatggaa tggactttat cgcaaaaacc tttagcgaaa agaaagtatt
     1381 aggaaattta gtaaataagt ttaaaaaaat tatagaaaat tgaaatattt aacaggaagt
     1441 gtatatagtc atgaagaagg ttttggtaat atcgtattat tatcctccgc gtccgggagt
     1501 tggaacaaac agaataaggg gactgacaaa atatttacac cggtttggtt gggaacccgt
     1561 tgttttaacc actgagttgg gtgagggaga atgggctcct tacgctaaag taattgaaac
     1621 agaattttgt tcaaggatag gcattataaa gaagtttttt ggtttcaagg atggaatagg
     1681 attacagcaa cagataggta taaataaaaa aaggaaaaac ggaattgtaa acagattttt
     1741 tagttattta aaagatttca ttgcttttcc cgacgaattt tgcacttgga taaaaaaagc
     1801 tgaaaaaatt ggaatgcagc ttattgataa tgagaaaatc tcggtaatta taagcagttc
     1861 cagtcctgta agcacacacc tgattgccag ccgtttgagc agaaagaaag gtgtcccgtg
     1921 ggtagcggat ttacgggatt tatggacaca gaatcattat catgatcata cacttataag
     1981 gagaatattt gaaaggaatc tggaaaaaag gacattgaga tatgcatcgg ctcttgtaac
     2041 tgtatcggag ccatatgcag aacaattgag gctgcttcat gggaataagg atgtatattg
     2101 cataacaaat gggttcgacc cggacgaagt aagtgattat tgtcctgcag aaaaaacagt
     2161 aagtgaaata actccagagg atatagttat agaggataca gttacagagg acatagttac
     2221 agcggataca gctgtggggg atacaataga aggcaggctt ttaatatctc atacaggatc
     2281 cctatacgat ttgaagaaaa atccgggacc acttttcaaa gttattagaa aacttattga
     2341 taatggatat atggatagaa gcaagatcca ggtgaatttc tacggaccct tctgtcataa
     2401 gttggaggaa ctgataaagg tatataagct tgaaagaatt gcagttcagc atggaattat
     2461 aaacaggaat ttaagtatgg aagtacagag aaaatcatca gttcttcttg ttatggaatg
     2521 ggataatcct ggtgaggaag gggtatatac cgggaaaatt tttgaatatc ttgctgccag
     2581 gagacctatt ttggctatag gagtaagggg aggggtgctt agagaccttc tgaatactac
     2641 aagagcaggg gtatatgcta cttctgaaat tcaaattgag gaatttataa aaagatggta
     2701 tgaagagtgc attaatggta cacaacctgt gtttatagga gataaggata aaattaacga
     2761 atattcccat gtaaatatgg caggtaagta ttcagagctt ttaaacagag tgtcaagctc
     2821 tatagattaa gtgggactgc atacggacgg ggccgcatat agatgaggca gcatatggat
     2881 gagataggat atagatgagg cagcatttag atgagatagc atataggtga agaaagtgaa
     2941 tttaaataaa ttaaaatata aatttaatag aataaacagc gaccctttgt tggaaatact
     3001 attagtactt atatttgcag tgaccactaa tactgtgata taccttaatt tagtgttgca
     3061 ttcaattctt gctatttctg ctattgcagc actctttttt atatttgcag actattccaa
     3121 tggtctggta cttatggtat ttctaatccc ccttgactct gcttcagtaa taataccggg
     3181 actaacctta actaaagtaa ttggaattgt gactgttatt tcatttttaa taagtaatat
     3241 aaaacatggg attaaattac ctgatatatc tttaacagcc ttcctactat atgcagcagc
     3301 atcaatagca tggtctatag atataaagga atccttcaac agaataacta atttggccaa
     3361 ttactatgtg atggtaatta tgataataaa ttcagtaaat ccaataactt caataaattc
     3421 aatagcttca ataaattcaa taagtacaaa taaaaactta ataaaatata tgaacgctta
     3481 tctttgtggg agtatagtag taagctgcct tggtatatac aatttcatta catccggcgg
     3541 aagccaaaga gtggctgctt tttatggcga aaatatgaac ttttatggtc tgacaatagg
     3601 gattgctatt attttaagtt tatatttatt taaaaactat gtaaattcag gcaataaaat
     3661 tttaaagggt atttacttgg cagcatgtct tgttttaatt ctggggcagt tggtttcctt
     3721 aagcaggact gcctgggtgg caacctgtat tagttctttt gttttcttta caagcagtaa
     3781 gtttaatatt aaaagggtta ttatttctat tataacactc gtggtaattg tatctgtacc
     3841 attgttaatt ttaaaaagga ttgttcctgt ttcgacagac ttatttttaa ataggttcac
     3901 cacaatattc accttaagtg ataatgcagc aggaagactt gatatatgga aagttgcctt
     3961 taacatattc aaagattatt tgttttttgg agtgggcata agtaatttcc ctgctgcatt
     4021 cacctacgga tatataatga atagcagtgc tcctttggat gtgggtttga attcggatcc
     4081 tcacaatttg tatataagca tttcagtaga gttgggaatt acagggatgc ttatattttt
     4141 gtttactatt tataatatat tcaggaatat tttttctttg aaagagaatg gctataagga
     4201 gttttgcatg gtgctcatgt tgttcttatt agttgccggt ttaacaagta ctattctata
     4261 taagaaatac ttttggtatg ttttgtctct tgtttatgca ataattaatc agattaatga
     4321 aagtgaaaag gcgaaaggga aatattaaat atggagaaaa aaagacttag gcttatagtt
     4381 atatctcata tgtttccaaa tagctatgaa ataaatggtg ggattttcat atatgagcag
     4441 gtaaaaagct tggcaaaact gagtaagtac aacataacag ttataagtcc cataccctgg
     4501 tctcctcata tgctatggtt caaggaaaaa tggaggaaat atgggcaaat agaagaaaag
     4561 gtaataatga ataaatcagg taatgtagat ggaaatttta atggcaatat ggatgtcaat
     4621 acagatgatc atattgatag caatatatat ggtaatacag atggtaatgg taataaagat
     4681 gacaatattg atgttatata cccaagatat atttgctttc ccggcaaaca cctgtttttc
     4741 atacgggcta ttacaatgtt tatatccgtt gctaaagtaa taagaacact tatgaaaagg
     4801 gatgatattg caattattca tactcatacc atattgcctg atggattttc aggggcactt
     4861 ttgaagaaat ttttccccaa tattaaaaca gtttgttctg caagaggaag tgatataaat
     4921 gtttaccctt acaggaataa gatggctatg tattttacca gatattcact cagaaacaat
     4981 gatattataa ccacagttag caaagatcta atgagaaaat gtctggaatt aagaaaaaaa
     5041 accaacaaaa ggctcaacaa cagacctggc agcgaactca actgcaactc ccgcagcaca
     5101 gtgaacccgg aagtgaatct agctgcgaat tcaaaagtag gctcggcaat gaacccaacg
     5161 gcgaactgcg tggtagtata taacgggctt aaagaagatt ggtttcattt atgtggtgag
     5221 gaacaaataa aaattgagga actgaaaaaa gagtatggtt ttaaaacaag acttttgttt
     5281 gtgggagaac tgtccaagga gaaaggaatt tatgagctta ttcttgcttt tgaaagactt
     5341 caaaaacgtt ataagaaaat aggattgcta attgtgggcg atggaagaga acgtaaggaa
     5401 tttacaaaca tgtgctctca aaaagacctg caggataaag ttattttttt cgggcaggtt
     5461 aaacatgaaa atgtgaagct gctcatgaga tcatgcgata tatttattct tcccagctat
     5521 tctgaaggta tgcctaatgt tgtgctggaa gtcatggctg ccggtaaggt ggtaattgca
     5581 acaagtgtag gaggagtcac agaagtagtt gaacataaat ataatggtat agtgattaaa
     5641 cctaaatctg tggaggacat agtaaattca gttgaattgc ttttgaatga taatgctctt
     5701 tataattcaa tatgtcaaaa tgcatacaca actgtaagga ataaattttc ctggaataaa
     5761 aatgcactgc agatagatgg aatatatgaa agccttttaa attagaggta aggagacaca
     5821 tattttcaga gtatagaatt ttcaagtttc ttgacaaatt cttatgctgg tgttcaacgc
     5881 agaaagagaa acagaatttt ggaggtattg cgtgtcagat gcttatatag tgagacaact
     5941 ttatgaaagt gaatatgata tatgggacaa tttcgttgat atatctcctc agggaagctt
     6001 atataataaa agttggtggt t
//
LOCUS       DAOSDD010000253         5985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4752_length_5985_cov_4.940978, whole genome shotgun sequence.
ACCESSION   DAOSDD010000253 DAOSDD010000000
VERSION     DAOSDD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_5985_cov_4.940978"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK733_16695"
     CDS             complement(<1..835)
                     /locus_tag="PK733_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12209.1"
                     /translation="MFNIILPKQYSIEDKLALMQDDAKFEIASDSDAESTALICASEV
                     KSISSPAKIPKMFLASKCVFNCSYCSCRCSREERNNYCNTPEELARLSVIAAKNNGHG
                     VFVTSAIYKNADYTQELLAESVHIMREKLYYNGFIHAKVMPGADPLLIARTGKYANRL
                     SVNIEVAKSSGYERIAKQKNKTNILTPMRNISSLIMSAKNEKRKFATSQTTQLMAGST
                     EEDDRTIMTLSQALYKTYRLKRIYYTAFQYKHEAKGYENENLKLTSTPYWRMARLYQA
                     DR"
     gene            complement(1050..1274)
                     /locus_tag="PK733_16700"
     CDS             complement(1050..1274)
                     /locus_tag="PK733_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12210.1"
                     /translation="MAEVKKVKVCKKCSGFDVAELKGKVKVKDHTTGCIGKCAQKCPE
                     LHGKVYGFLNGEFTVCDTKEEFFEKIAEII"
     gene            complement(1515..2234)
                     /locus_tag="PK733_16705"
     CDS             complement(1515..2234)
                     /locus_tag="PK733_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12211.1"
                     /translation="MNLDKKTINKSITILAIFIFTCILIRIGIAIWKNYNLVNNFIAN
                     PNEPYQQRIPIAEEKFIDDYIYRLYFPFATDLLGRPNTFVLKKPISYYSAPDDNLEPV
                     ITIDSGKKYIITTNDINSYKYGIQSWPTYKKGWRYVIPFAEVGNEKSVTSAYFVKLED
                     LFGSLKSIKLYYHMPKGYNNRRTLLIKDEMLYKEGYYLSPDLLMPIIDSWNITLLASA
                     FFLIIIRIIFRKITTFQERKK"
     gene            complement(2430..3221)
                     /locus_tag="PK733_16710"
                     /pseudo
     CDS             complement(2430..3221)
                     /locus_tag="PK733_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184583.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
     gene            complement(3459..4274)
                     /locus_tag="PK733_16715"
     CDS             complement(3459..4274)
                     /locus_tag="PK733_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12212.1"
                     /translation="MKKFLLIILIICFVSLIGCTYFKNKLIGRSMYQNQEATFKQDAQ
                     EAYNNGGDNQEIISESDKEKIGKKFIEPIYIDIESPDKTMRVISNDSGLVLVSHEHDI
                     LKVIITKEQQIGQGFEPVWSPHSNKICITQYGEEFSTFSIYDVKNDTSTPISMAKGLA
                     KTEILPDSPYDWYPLTCFVGWLDNERILFDVSHQVPLDGSNPTTYERGCRKDVYVYSL
                     KDNLFSNLTNSYDGEYYMLKEINYERNVLIIDIFVWRNKNVEIEKKNEIEVPF"
     gene            complement(4332..4841)
                     /locus_tag="PK733_16720"
     CDS             complement(4332..4841)
                     /locus_tag="PK733_16720"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ12213.1"
                     /translation="MAEVFNEKTVSFELKESLISDYSWYTSPKIMRLVKSEHLQFDIR
                     SLESDKFSYPYHFHRNSEEIFVILSGSVTLRTPEGFKQLDEGDIVFFKMGPEGAHQLY
                     NHTKKPCRYLDIRTVIGLDVCEYPDSGKINILPYQEIYQSINKVDYYIDEDKVREKWP
                     KEILSKING"
     gene            complement(5377..5517)
                     /locus_tag="PK733_16725"
     CDS             complement(5377..5517)
                     /locus_tag="PK733_16725"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOJ12214.1"
                     /translation="MKLRKTGKIEKTVIVPNHYEVAKGTLQSILEQAGISLEKFLKKI
                     NK"
     gene            complement(5610..5819)
                     /locus_tag="PK733_16730"
     CDS             complement(5610..5819)
                     /locus_tag="PK733_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOJ12215.1"
                     /translation="MSFKVTVIVQKDDNWYVAKCLENSVASQGKSVEEAINNLREALE
                     LYYEDSTPDMISPKQTFVTTLEVTV"
BASE COUNT         1732 a         1183 c          928 g         2142 t
ORIGIN      
        1 gcctatctgc ctgatataat cttgccatac gccaataagg tgttgaggtt agctttaaat
       61 tttcattttc ataacccttt gcttcatgtt tatattgaaa cgctgtataa taaatgcgtt
      121 ttaaacgata tgtcttatat aatgcctgag acaaagtcat aatcgtccta tcatcttcct
      181 cagtactgcc tgccatgagt tgtgttgttt gagaggtagc aaatttccgc ttttcatttt
      241 ttgcactcat aatcaagcta gagatattac gcataggtgt caaaatgttc gttttgttct
      301 tttgttttgc aatgcgttca taacccgaac ttttggcaac ctcaatatta acacttaacc
      361 tgtttgcata ttttcctgtc cttgcaataa gaagcggatc ggctcctggc ataacttttg
      421 catgtataaa gccattgtag taaagcttct cacgcataat atgtacactt tcagcaagaa
      481 gttcctgagt gtagtcagca tttttatata ttgcagaggt aacaaatact ccgtgtccgt
      541 tgttttttgc tgcaataaca gataaccttg caagttcttc aggagtattg caataattat
      601 ttctttcttc acggctgcac ctgcaactac aataggagca attgaatacg catttgcttg
      661 ctaaaaacat cttgggaatt ttagcgggtg agcttatgct cttaacctcg ctcgcacaaa
      721 taagtgctgt gctttcggca tcactatcac ttgcaatttc aaattttgca tcatcctgca
      781 tcaatgcaag cttatcctct atgctatatt gtttcggtaa tataatgtta aacacaaaat
      841 tcacatccat atttatatgc tatcaatata gttattcctt accgcaagca cctttcaatc
      901 atttcctctt aaagcttcag ttccgtcagg ttatagattt taataaaata ataaatatac
      961 cattcatact tttgtaaagc catcttataa tcctttaatt tattgtagtg cacgcttatt
     1021 ggtttgcagc taactgttgc ggtatgtaat cagataatct cggctatttt ttcgaagaat
     1081 tcttccttcg tatcgcacac agtaaattcg ccgttcaaga aaccgtaaac cttaccatga
     1141 agttcgggac atttttgtgc gcacttgcct atgcaaccgg ttgtgtggtc ttttacctta
     1201 accttgcctt tcaattctgc aacatcaaaa cctgaacatt ttttacaaac ttttaccttt
     1261 ttgacttctg ccatttacat tcttcctccc ctatatttta attactgcac ttgacaatgg
     1321 tgtgtcacat taatatactc tgcaaattat tttagtaaat tttaaagtgg aatataaatt
     1381 gaattttgtt gcttgtttag ttattctgaa ttatatcaaa cacatgttcg tttgtcaata
     1441 agatttagct aaaatttaaa taatttttcc aaaatccttt atgcatttgg ccatgtaaaa
     1501 caataatcct catattattt cttcctttct tggaaagttg taatttttct aaaaattatc
     1561 cgaattataa tcaaaaagaa agcactagct aaaagagtaa tgttccaact atcgataata
     1621 ggcataagca aatctggcga caggtaatat ccctctttat acagcatttc gtctttaatt
     1681 agtaaagtcc tcctattgtt atacccttta ggcatatgat agtatagttt gattgacttt
     1741 agagaaccga ataaatcttc tagttttacg aagtaagctg aagtgaccga tttttcatta
     1801 cctacctcag caaatgggat tacatatcgc catcccttct tatatgtagg ccagctttga
     1861 attccgtatt tgtaagaatt gatatcattt gtggtaataa tgtacttttt ccctgaatct
     1921 atcgtaatca ctggttctag attgtcatct ggtgctgagt agtaactgat tggcttcttt
     1981 agcacaaaag tattaggtct gcctaaaaga tctgtagcaa aaggaaaata taatcgataa
     2041 atataatcat cgatgaattt ttcttctgct ataggtattc tctgttgata tggttcatta
     2101 ggattagcaa taaaattgtt gactaaatta tagttcttcc atatagcaat accgattctt
     2161 atcagtatac atgtaaagat aaatatagcc aaaattgtta tgcttttatt gattgttttt
     2221 ttatctaaat tcataaacaa ccccccaata caattttcat tttcaattgt taaattcctt
     2281 ttttgcatta tgaacttata taacatagtc gagttgacca catagccctt ctccctggcg
     2341 gtgttcgtca cccggcggat cggtgtagca tgttacagag acctttattt gggtatccag
     2401 gtctattata tgatgtatct gccccagagt catgcctgag tactttcttc tccatcgtta
     2461 agcaacggtt catacatcat aatggcatga ttctctgcaa cgacctcatc tgaaatcaat
     2521 tttgcactgc tatccaagac ctttcgccta attatattat gtcgtgtgta accgcctcac
     2581 cattcgtgtt ttattatgtg ttcactttcg tagatttgga attcaagtgg acagggatgt
     2641 ttacatctcc attcaccttc caaatgatta tctcctactt taaccagaag ttgatattca
     2701 tgtggagtat cattccttat ttgtaaatcc acataattat atacaattgt agcaccactt
     2761 ccgaagggtt gagttcgatt ggaatctgga aaaacatcat gtgtgtggcg ccagcgttcc
     2821 ttgacagtta atggtgtatg aagagtcatc caatagatga ggtttgagag ttggcataag
     2881 ccgccaccca cacccgcatt aaatgagccg ttagtcaaaa ccattccttt taaatagcct
     2941 tttgcttgtg tcggtttccc taccaagcgc caaaaggaaa aagtctctcc cggctttagt
     3001 atcaccttat taagccttcc acaagccagc atcaagttag ttactttatt ctcctgcagc
     3061 cacatatcaa cattgcgtaa ttgtctatat aatggtgttt tgtgggctgc cactacattg
     3121 aaaagctcaa tttctcttga tctagaagga tatctgattg atcggttaaa tatccactga
     3181 aaatatcgcc gacaagtaaa gtataacctc cccaatgtca ttcgtattat tgaacgaggt
     3241 gctggctttc tagattgata atggtaattc aaaccatatg cctcctttta tctctttgcg
     3301 aatatttcgt ataacggtcc atagcaatag gtaaggcttt aatgctgttt atggactaag
     3361 gtcggatgac atcgctcctg taccctcgca aagatacatt gttatgtaaa gtcgcacgcc
     3421 cttatagtca acagataaaa ggacgccggc ctatttaact aaaacggcac ttctatctcg
     3481 tttttctttt caatctcgac atttttattc ctccagacga agatatcaat tataaggaca
     3541 tttctttcat agttaatctc ttttaacatg tagtactctc catcgtaact atttgtaaga
     3601 ttagaaaata aattatcttt taaagaatat acatatacat ctttcctgca acctctttca
     3661 tatgtcgtag gattactgcc gtctagaggt acttgatggc ttacatcgaa taaaattctc
     3721 tcattgtcta gccatccaac aaagcatgtc aacggatacc agtcataggg tgagtccggt
     3781 aaaatttctg ttttggctag ccccttagcc atactaatgg gagttgatgt atcattttta
     3841 acatcatata ttgaaaaagt cgaaaactct tctccatatt gtgtaataca tattttgttg
     3901 ctatgcggtg accaaacagg ctcaaatcct tgtccgattt gctgctcctt agtaataatt
     3961 actttgagta tatcatgttc atgagagact agtactagac cagaatcatt tgaaattact
     4021 ctcattgttt tatctggaga ttcaatatct atatatattg gctctatgaa tttttttcct
     4081 attttctctt tatcgctttc ggaaattatt tcttgattgt caccaccatt attataggct
     4141 tcttgtgcat cttgtttaaa tgtagcctct tgattttggt acatacttct accaattagc
     4201 ttatttttaa aataggtaca tccgattaag cttacaaaac atatgattaa aattatcaat
     4261 aagaactttt tcaaaattat ttcaacccct tgataggcct ctcaatatat ccgctacacg
     4321 atgtggtgac cttatccatt tattttactt aaaatttctt ttggccattt ttcccttact
     4381 ttatcttcgt ctatataata atctacctta tttatgcttt gatatatttc ttgatacgga
     4441 agaatattta tctttcctga gtctgggtat tcacaaacat caagtccaat tacagtacga
     4501 atatcaagat acctacatgg cttttttgta tgattatata gctgatgagc accttctgga
     4561 cccattttga aaaatacaat atctccttcg tctaattgct taaatccttc aggcgtacgt
     4621 aatgtaacac tacctgataa gataacaaat atctcttcgg aattacgatg gaaatgatat
     4681 ggatatgaaa acttgtctga ttcaagagaa cgaatatcaa actgcaaatg ttcggattta
     4741 acaagtctca taattttggg gcttgtatac catgaataat cagatattag agattcttta
     4801 agttcaaagg ataccgtttt ctcattaaat acttctgcca tataagtcat cctcacaaat
     4861 tcatattttt tctagtagtt tacgttaccg cttttttaaa atgctccgat tacggagtca
     4921 ggggcaataa cttagtcgtc atttcgtaca acgtggtgtg ctccttgata ttattatatc
     4981 atttggggca attatttaaa tgtttttact aaaatgtttt tctaaaacat acgtaacacg
     5041 atgtctgctt atcgtctggt agtcgcgatg gcgatgagct ggacccgcag aactcttatt
     5101 cctagcggtg ttcgtcaccc agcgaatcgg tgtggtgcac aagctccaga gtccttccca
     5161 ctgcactttc atccaacggc cagcactcct gatgctttct gagccgcttt tggaactcaa
     5221 aggcaagcct gttcggcgaa aaagagttgt gcctgctcgt gcttctgccc acacctattt
     5281 taattaaagc cagcaacctt cacttgagtg ctttattata cgtagtacag cggcttcgcg
     5341 ttcagaaaga gccatggaag gcgctccctc aaatgtttac ttgttaattt tcttaagaaa
     5401 tttttcaagc gatataccag cttgttcaag gatactttgt aatgttcctt tagcaacttc
     5461 atagtgatta ggaacgataa cagtcttttc tattttacct gtttttcgta atttaatatg
     5521 atttcctttt tatgaaataa ccttgaatcc aaatttacct aatatagata tgattttttc
     5581 aggctttagt actggatatt ttgaacccat tagactgtta cctccagtgt tgtaacaaat
     5641 gtctgttttg gactaatcat atcaggtgta gaatcttcat aatatagttc caaagcctct
     5701 cttaaattat ttatagcttc ttctacagat ttcccttgag aagcaacact attctccaaa
     5761 cattttgcaa cataccaatt atcatctttt tgaactataa ctgttacttt gaaactcata
     5821 aactcacact ccaacaattc ttaataagtc tattttacca caaaacaaac atacatttac
     5881 attataaaat aaaatttata taaccttcta tatagaacag gttttaaaac atttaaaact
     5941 tgcttctgtt accttgatgt aacttacgat ttgcaaattc ttcat
//
LOCUS       DAOSDD010000254         5957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4783_length_5957_cov_4.405117, whole genome shotgun sequence.
ACCESSION   DAOSDD010000254 DAOSDD010000000
VERSION     DAOSDD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4783_length_5957_cov_4.405117"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK733_16735"
     CDS             complement(<1..474)
                     /locus_tag="PK733_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ12216.1"
                     /translation="MKILVLSDTHGDIDNAREAIKKNNDINLIIHLGDYFRDAQKLSD
                     EFPNIPIEYIYGNSDFMIDETPAEKVLEFEGKRIFITHGHRYSVKWDYEKLFKKAYEV
                     SADILLFGHTHVADLVKNGRCFVLNPGSISDPRDNSSESYAIIEINNGGVMPKLCY"
     gene            complement(545..1150)
                     /locus_tag="PK733_16740"
     CDS             complement(545..1150)
                     /locus_tag="PK733_16740"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOJ12217.1"
                     /translation="MRKFIVATRNKGKLYEIKEILSDFPYDVVSMEEAGISNEIDEYG
                     NTFEENAMIKANEVWKLTGGTVMADDSGLEVEYLNGAPGVYSSRFGGENATDEDRNKK
                     LLDLLKDVPFEKRKARFVCVIAVIYPDGTSFTVKGACEGYIACAPEGNNGFGYDPLFY
                     MPEFGMTTAQMNPCEKHKISHRGKALRMMVDELKKRENETL"
     gene            complement(1113..1895)
                     /gene="rph"
                     /locus_tag="PK733_16745"
     CDS             complement(1113..1895)
                     /gene="rph"
                     /locus_tag="PK733_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOJ12218.1"
                     /translation="MRTDGRENIEIRPVKITRNYIKYAEGSVLIEMGNTKVICTASVE
                     DKVPPFKKDTGEGWITAEYSMLPRATQVRNQRDISKLRLSGRSNEIQRLIGRSLRSVV
                     NFNVLGERAVTIDCDVIQADGGTRTASITGSFVALADACNSMVEKGIIEKIPLMDFVA
                     AISVGIVNGIETLDLCYEEDSNAQVDMNVVMSGKGDFIEVQATGEQSPFTKKQLDQML
                     EFAEKGIKELVSIQKEVLGQLADKIGGVTKDEKIYSSNTEQR"
     gene            complement(1935..3209)
                     /locus_tag="PK733_16750"
     CDS             complement(1935..3209)
                     /locus_tag="PK733_16750"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ12219.1"
                     /translation="MMIKKRRFIRLWFLLLVLIILLASGCESSEILDVNNNLQPTESS
                     DSGIINGIVSYAENNSDTVGTAEESKRKSNDSIDEKDSKEVEKENKNTADYKTDIDEY
                     KTNADKEAQENIFIEVYYQDLEGFVIPVTRRIPRQLSVARAAVNGLVDTPVNREEMAY
                     FGLIPILPKGTVFTINLKDKTAVIDFNSRVLDYTSEKSEYNIVSSIVYTLTQFESIDD
                     VIILINGYEQEKLRYGTDISGRLSRKNILVNAVDANKVNLKKGLEKIDVYLLRSLRDV
                     GNKGNEGSVDNTGKNDNVFLFPVSVETEKVNTNKFIANIMRYLSSDEQKPEIFSAVKP
                     GIMLNESTIEDGLLTLDIGFDNVSYGGTLNEYLMVNQIMHSMRQISGVNRIRFLIDGK
                     AITLPEGTDISKPVLLPAKINDIIDDEVFVSR"
     gene            complement(3419..5215)
                     /locus_tag="PK733_16755"
     CDS             complement(3419..5215)
                     /locus_tag="PK733_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ12220.1"
                     /translation="MRFLKTILILVFITIFATSSLLVHAAPQPPYALLSDVRHFTGNE
                     CAGKYEGKFECVSISFSAERDFSTFTLTNPDRIVIDVKDALYDGATKTIKGGDIVQSI
                     RYAQNEKNIVRIVLDTKGRQNYKLEREKERINIYVGDIIEKTGKDAGEENEVKQPENE
                     SQQAGEQNKERSRDEQNREQNPASRGSVDRGQSSAEDSLNIGFASKESVDEVSIFLDN
                     YKGYNISTLSDPDRIIIDIPGVIAPVLQQEIKVNGTQVRAVRYAQYSENTARVVVDTI
                     QRCPFNIKEYDGQLVLNVESPKFNNITYNNNGDRVYLSIQGASLTEGGQDLRKFYTEK
                     YSSDGTVYILSFRSSLVNLPQGVIQVNDRYLESITISKSLFSFYTDIIFKAKDKFVYQ
                     VMARPDLKDTAITILKPASKSDKLVVIDAGHGGVEPGAVYGSLQEKNLNLDIALRLNE
                     LLKKKNVKTYMIRENDSFVGLYERAYIANNLNASLFLSIHNNAMEDRSFGGTMTLYYV
                     TGSESKGFNSYKFSNIIQNELIKTMGTKDRKVRERPELVVLKATKMPASLAEIAFMTN
                     SDDRAKLQNSEFKQKAAQALCDAVIKSLSEIK"
     gene            complement(5617..>5957)
                     /locus_tag="PK733_16760"
     CDS             complement(5617..>5957)
                     /locus_tag="PK733_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOJ12221.1"
                     /translation="VHYRDDFQQKTWEEFEGPGEERCAMCYTIRLDKAASFAKEHGFD
                     AFTTTLLISPYQKHDLIKELGEKLADKYKIQFYYRDFRPGFRKGQQQAKELGLYRQKY
                     CGCIISLYNK"
BASE COUNT         1683 a         1278 c          796 g         2200 t
ORIGIN      
        1 ataacataac tttggcataa ccccaccatt gttaatttct attatggcat atgattcact
       61 tgagttatcc cttgggtcac taatgcttcc cgggtttagt acgaaacaac gtccattttt
      121 aaccagatct gcaacatgtg tatgcccaaa taacaggata tcagcactta cttcatatgc
      181 ttttttaaaa agcttttcat aatcccattt aacagaatac ctgtgaccat gagttataaa
      241 aatcctttta ccttcaaact caagtacctt ttctgcaggg gtttcatcta tcataaaatc
      301 actattgcca tatatatatt caattggaat atttggaaat tcatcactta gtttttgtgc
      361 atctctgaaa taatctccca aatgaattat caaatttatg tcgttattct tcttaattgc
      421 ttcccttgca ttatctatat caccgtgtgt atcacttaaa accaatattt tcattttaaa
      481 ccctcttcac ttaaattaag tatttatact gcatatatta tgattatggc tgaacttatg
      541 tttttcacaa ggtttcattt tctcgttttt tcagttcgtc aaccatcatt cttaaagcct
      601 ttcccctatg actaatcttg tgtttttcac aaggattcat ttgagcagtt gtcataccaa
      661 attcaggcat ataaaataag gggtcatatc caaaaccatt atttccctca ggtgcacaag
      721 ctatataccc ctcacatgct cctttaactg taaaacttgt gccatcaggg tagatcactg
      781 ctattacaca tacaaatctt gcttttcttt tctcaaaagg tacatctttg agcaaatcaa
      841 ggagtttctt atttctatct tcatctgtag cgttttctcc tccaaaccta gaagaataaa
      901 caccaggtgc cccatttaaa tactcaactt caagtcctga gtcatctgcc attaccgtcc
      961 cgcctgtcag cttccatacc tcatttgctt ttatcatggc attttcctca aaggtattgc
     1021 catattcatc tatttcatta ctaatacctg cctcttccat ggatacaaca tcataaggaa
     1081 aatctgaaag aatctctttt atttcataga gcttaccttt gttccgtgtt gctactataa
     1141 attttctcat ccttagttac tccccctatt ttgtcagcca gttgtccaag aacctctttc
     1201 tgtatagaaa ctaattcttt tattcctttt tcagcaaatt ccagcatttg atccagttgt
     1261 ttcttggtaa agggtgactg ttcacctgtt gcctgaacct ctataaagtc tcctttccct
     1321 gacattacaa cattcatatc cacttgagcg ttggagtcct cttcataaca aagatcaaga
     1381 gtctctatac cattaacaat gccaacactt atagcagcta caaaatccat caaaggaatt
     1441 ttctctataa tacccttttc caccatacta ttgcaggcat ctgcaagtgc tacaaaactt
     1501 ccggtaattg atgcagtcct tgttcctcca tcagcctgta taacatcaca atcaattgtt
     1561 actgctctct cgccaagaac attaaaatta actactgacc ttaaggatct tccaataagc
     1621 ctttgaattt cattgcttct tccacttaag cgcaatttgc tgatatccct ttgatttcta
     1681 acctgagttg cccgtggaag cattgaatac tcggcagtta tccatccctc gcctgtatcc
     1741 tttttgaatg ggggcacttt atcctctaca gaagctgtac aaataacttt tgtattcccc
     1801 atttcaataa gtacagatcc ttctgcatat tttatatagt tccttgtgat tttaacaggc
     1861 cttatttcta tattttctct tccatctgtt ctcaatcaac ccgtcctgcc tttccttatt
     1921 aatattaaat aaagttagcg tgaaacaaaa acttcatcat caataatatc attaattttt
     1981 gctggtaata gtacaggttt tgaaatatca gttccttcag ggagcgttat ggctttccca
     2041 tcaattaaaa acctaattct atttactccg ctaatttgcc tcatggaatg cattatctga
     2101 ttaaccatta agtattcatt taaagtgcct ccatatgaaa cattatcaaa acctatatca
     2161 agagttaaca atccatcctc tattgtactt tcatttaaca taatacctgg ttttaccgcc
     2221 gaaaaaattt ctggtttttg ttcatctgag gacaaatacc tcattatatt tgctataaat
     2281 ttattagtat ttaccttttc agtttctaca gaaacaggaa acaggaatac attatcattt
     2341 tttccagtat tatcaacgct gccttcattg cctttatttc caacatccct taaggacctt
     2401 aacaagtaaa catcaatttt ttcaagtcct tttttcaaat taactttatt tgcatcaacg
     2461 gcgttaacaa gtatgttttt cctgcttaat cttcctgaga tatccgtccc atatctcaac
     2521 ttttcttgtt catacccatt tattagtata attacatcat caattgattc aaattgtgta
     2581 agtgtataca ctattgatga aactatatta tattcggatt tttcactggt ataatcaagt
     2641 actctgctat taaaatcaat aactgctgtt ttatctttca agttaattgt aaacacagta
     2701 ccctttggca gtattggaat taaaccaaag taagccattt cctctctgtt tacaggtgta
     2761 tcaaccaaac cgttaacagc tgctctggct acacttaact gccttggtat tcttcttgtt
     2821 acaggaatta caaatccctc taaatcctga tagtatactt cgataaaaat attctcttgt
     2881 gcttctttat cagcattagt tttgtactca tcaatatcgg ttttataatc agcggtattt
     2941 ttattttcct tttctacctc cttgctgtct ttttcatcaa tactatcatt gcttttccgt
     3001 ttactctctt ctgcggttcc aacagtatct gaattgtttt ccgcatatga aacaatgcca
     3061 tttataatgc cagaatcact gctttcagtt ggttgcagat tattattgac atctagtatc
     3121 tcactgcttt cacacccgga tgcaagtaaa atgatcagca caagcaagag aaaccataat
     3181 cttataaatc tccttttttt tatcatcata tacgtccatc ctttaactaa ctaatttttc
     3241 aaacttttta aataatcggc ttttagtata attatagaca tatatgatgt tttttaatca
     3301 atttaaggta atgttaccac aatcctatag caaaaaaaat gagtagattg tataaaatct
     3361 tactcatttt tttattaatt tttatggact gcactttaat atttgtttta ctatatcctt
     3421 actttatctc tgacaaagat ttaataactg catcacataa agcctgggca gctttctgct
     3481 taaactcgga gttttgtaac tttgctctgt catctgagtt tgtcatgaaa gctatttctg
     3541 ccagagatgc aggcattttc gtcgctttaa gaacaaccag ttcaggacgt tcccttacct
     3601 tcctgtcttt cgtccccatg gttttaataa gctcattttg aataatattt gagaatttat
     3661 agctgttaaa acctttgctc tcacttcctg ttacatagta gagagtcatt gtaccaccaa
     3721 aagaacgatc ctccattgca ttgttatgta tgcttagaaa taatgaagca ttaaggttgt
     3781 ttgcaatata agctctttca taaagcccta caaaactatc attttctctt atcatatagg
     3841 tttttacatt tttcttcttc aaaagctcat ttaatctcaa agcgatatca aggtttaaat
     3901 ttttctcctg caaactgcca tatactgccc ctggttcgac tcctccatgc cctgcatcta
     3961 taaccacaag tttgtccgat tttgatgcag gcttaaggat tgtaattgca gtatctttta
     4021 aatcaggtct tgccataacc tggtacacaa atttgtcttt agctttaaaa attatatctg
     4081 tataaaacga aaatagactt ttacttattg ttattgattc aaggtatctg tcattgacct
     4141 gtataacccc ctgaggtaaa ttaacaagcg agcttctgaa agacaatatg tacactgttc
     4201 catccgaact gtatttttca gtataaaact tcctcagatc ctgtccgccc tcagtaagag
     4261 atgcaccctg tatggaaagg tataccctgt ctccgttgtt attatatgta atattgttaa
     4321 atttgggact ttcaacattt aaaactaact gcccatcgta ttcctttata ttgaagggac
     4381 atcgttgaat tgtatcaaca accacccttg cagtattctc tgaatattgt gcatacctta
     4441 cagctctgac ctgagtaccg ttaaccttta tttcctgctg caaaacaggt gcaattacac
     4501 ccgggatatc tataataatt ctatccgggt ccgataaagt tgagatatta taccccttat
     4561 agttatcaag gaatatggat acttcatcca cactttcttt tgacgcaaat ccaatattaa
     4621 gggaatcttc agcagaactc tgtccgcggt caacacttcc acggcttgct ggattctgct
     4681 ctctgttctg ttcatctctg ctccgctctt tgttctgctc tccggcttgt tgactttcat
     4741 tctcaggctg tttaacttca ttttcttcac cggcatcttt acccgttttt tcaataatgt
     4801 cccctacata tatatttata cgttcttttt cccgctctaa cttataattt tgtctgccct
     4861 ttgtatcgag aacaattctt acaatattct tttcattctg ggcatacctt atagattgta
     4921 ctatatcacc gccttttatc gtcttcgttg ccccatcgta caaggcatct ttaacatcaa
     4981 ttacaattct gtcgggattt gtcagtgtga atgtactaaa atctctttcg gcactaaagg
     5041 aaatagaaac gcactcgaat tttccttcat atttccctgc acattcattg ccggtgaaat
     5101 gccgtacatc cgaaagcaat gcataaggag gttgcggagc cgcgtgtacc agcaaactgc
     5161 ttgtagcaaa aatggtgata aatactaaaa ttaaaattgt tttaagaaat ctcatgcttt
     5221 ttacctcaca ataaatgata tttttctttt gtaaattaga gtatatagta catatatggt
     5281 atatataata atatatccgg ttactataga ttattttatt acactttacg acaatttgca
     5341 tgaattttcc tatattttta ttaaatttta acttttctgt aacatttgcc gttttttttg
     5401 cattctattg taatattcgg tattctcccc atttaatatc tataacctga ggtgcattta
     5461 tttaagggct ctatgcactg taacaacagc ctctgttctc agttcttaat gtgcagcgac
     5521 tattttggca tcacataaca tgtcttccat ataatgactt aaatttacac ctatgataaa
     5581 tgcaccttat ggcttgctgc ttttatttat tatttattat ttattatata aggaaatgat
     5641 acatccacaa tatttctgtc tgtataggcc caactctttt gcttgctgct gaccttttct
     5701 gaatcctgga cggaaatccc tatagtaaaa ctgaatttta tatttatcag ccagtttttc
     5761 acctagttct tttattaagt catgcttctg ataaggactt attaggagag tagtcgtaaa
     5821 agcatcaaat ccatgttctt ttgcaaatga tgcagcctta tccagtctaa tagtataaca
     5881 catggcacaa cgctcttcac cgggtccttc aaattcttcc catgtttttt gctggaaatc
     5941 atctctgtaa tgcactt
//
LOCUS       DAOSDD010000255         5946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4794_length_5946_cov_4.705143, whole genome shotgun sequence.
ACCESSION   DAOSDD010000255 DAOSDD010000000
VERSION     DAOSDD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_5946_cov_4.705143"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PK733_16765"
     CDS             complement(<1..830)
                     /locus_tag="PK733_16765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ12222.1"
                     /translation="MRDVICSIIIAARNEESYIERCIESLENQSYDRRKYEIIVVEGM
                     SEDNTFKIVEVLKSKYNNIILLRNSRKIAPVAFNTGIKESAGRYVFIVGAHAEYPSDF
                     IEKSISGIKENNVDCVGGRIINIGRSKLGKVFSTVRNTAFGGGLSPYRYSDKKRFVNT
                     VAFGCYSRDILDRVGGFNEDFVRNHDNDLNKRIIQAGGKILFEPEIKFYYFTRNSLKD
                     ILRQMFSYGFWEAKLIKKHKEQLAITTLIPMLFVLYTIFAVAILIFRRTIIPLLLELV
                     P"
     gene            complement(953..1636)
                     /locus_tag="PK733_16770"
     CDS             complement(953..1636)
                     /locus_tag="PK733_16770"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOJ12223.1"
                     /translation="MIVSIHQPNYIPWMGYFYKIYKSDIFIFLDDVQYIKGSYINRNK
                     IKTHMGEHWLTIPSKFNDSSRCKINEVCINNEQDWRSNHLKNIHYNYKRAAYFNEFIP
                     FIQNCLSYNSDNLSDFNINTITKICGLLNIKTRIMLSSQLNIEEKSTMRIIKICEAVG
                     GTKYLSGRGGTKYQDPQMFMDYGIELEYSDFYEKPYSQLWGEFAEGMSIIDYILNCGT
                     DIKSFWNVL"
     gene            complement(1696..2325)
                     /locus_tag="PK733_16775"
     CDS             complement(1696..2325)
                     /locus_tag="PK733_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ12224.1"
                     /translation="MLFKRVLVLAPHTDDGELGCGGTIDRLVNSGTEVHYAAFSIAER
                     SVSEEYPSDILGTEVRNATAILGISKDCLHIYNYDVRNFQKCRQEILEDLVILNRSID
                     PDIIFLPSINDIHQDHHVIAREGVRAFKFKSVMGYELPWNNLTINTACFVELDYENIN
                     NKIRALGCYESQQGKKYFSSEFIKALAVTRGTQIGVQYAEVFEVIRWII"
     gene            complement(2394..3743)
                     /locus_tag="PK733_16780"
     CDS             complement(2394..3743)
                     /locus_tag="PK733_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ12225.1"
                     /translation="MIELYFHLEDLSFRNKCRYVIKTLLMISGISMDNDISISCESNS
                     IGKNGYYIGESISSISESCNCTGQGCNKKSCNINNSYERKYDVVISDYVINEILEVIT
                     CSEEYGEKLKDSRGRFLAKYSKRKDLYIPVINYYANGLKEALERAANNKGMNLKIAER
                     EFTVVLTHDVDNITDRNPYVFLHRIRNGLAKIKAGKILEGTRNILFAFKRIFIRDDAY
                     LDFEKFMELEEKYGFKSTFYFMNGRRGRYGARYNLEEVVDIIKKIELSGWESALHTNY
                     FSYNNSLKIKEEKARIEEITGKMVYGCRNHYLRFDVPKTWHKIEETGMRYDTTLGYAD
                     CEGFRAGVAFPFYPYDLAKDRMIEVLEIPLTIMDQTLVNVSLKDKEKAWEKIKRILDE
                     TRDVKGAIAVNFHTGVFYDRDYPGWKDIYIKVLKYLKEYGGRGITARELYKSLKLEN"
     gene            complement(3849..5036)
                     /locus_tag="PK733_16785"
     CDS             complement(3849..5036)
                     /locus_tag="PK733_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ12226.1"
                     /translation="MRVCMLTSGHGPLDDRIFYKEVLTLKKEYNDITIIAQSDREYNE
                     IGGVKIIGIPVRKNHGLKNRIETLVQLYKKGIEAKADVYHCHEPDALYIGYKLKKRLN
                     SMLIYDSHEYHPETFALHFKGIAKQVIKKLVYWFEGYLARKCNYIITVNRILVSKFKK
                     YNKHVLNLPNYPRLSIVKPFIGDEVACKDLRGLVYAGGISSDKGCIKILEAINKSRNN
                     SRMTFIGKAGSEFGNAAASLLGINGQLPFPLEKQKFFIGGSCKNNCENTVEFTGVVEH
                     KNVFELLKRNIIGFVLLQPVDPVDANCQPIKLFEYMACGLAVIAGDYPLIKEIVGDER
                     CGLLVNPSDENEVAAAIDYMLENVEETLKMGERGRQAVMEKHNWESIENGLLNIYKYL
                     SGC"
     gene            complement(5061..>5946)
                     /locus_tag="PK733_16790"
     CDS             complement(5061..>5946)
                     /locus_tag="PK733_16790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ12227.1"
                     /translation="SIYRRFYRNPSLTPTLGILLNDFAVMKPVNKISKEISVMCALIE
                     KLPSYLLFDYVFSENYINCLPFKWEKFRFDIEYAYVIRNLRALETELGKARLNEIFNQ
                     FKGPVKEKIKKAIKSGIRITTEDFSIDRFYELDNGTYMRQNSNITYTKEFLVNLDNAL
                     KERNSRKILFAVDKDNNILGANYIVYDRKAAHGVAMCSSEEGRKLGIPSLLVWEAIKF
                     ASEVSGELNFEGSSIKSVELFLRGFGGELIPRLRINKCSWLIRTMINILKRHKGIVQK
                     VIVKKAIVKKAIVQKKIM"
BASE COUNT         1728 a         1293 c          792 g         2133 t
ORIGIN      
        1 ggcaccagtt ccagtaataa gggtattatt gttcttctaa aaatcagtat ggcaactgca
       61 aatattgtgt atagtacaaa aagcattggt attaatgttg taatagccag ctgctctttg
      121 tgctttttaa ttagctttgc ttcccaaaag ccatacgaaa acatctgacg caaaatatct
      181 tttaaagaat ttctggtaaa gtaatagaat tttatttcag gctcaaaaag aattttgcct
      241 ccagcttgaa ttattctttt attcaaatca ttatcatgat ttctcacaaa gtcctcatta
      301 aagcctccaa ctctatctaa aatatccctt gaataacagc caaatgcaac ggtattaaca
      361 aaccgttttt tatcagaata tctgtaagga gacagccctc caccaaaagc agtgttcctt
      421 acagttgaaa aaactttccc cagtttgctc ctacctatat ttattatcct tccccctaca
      481 caatcaacat tattttcttt tatgccggat atactttttt ctatgaaatc agaaggatat
      541 tctgcatgag ccccaacaat aaatacatat ctgcctgcag attcttttat tccggtatta
      601 aaagctacag gagcaatttt gcgggaattt ctcaacaaaa taatattgtt atactttgac
      661 tttagaactt ctactatttt aaaggtatta tcttcagaca tcccctcaac cacaattatt
      721 tcatatttac gccgatcata cgattgattc tcaaggcttt caatacacct ttcaatatag
      781 gattcttcat ttctagctgc tataataatt gaacatataa catctctcat atttaacccc
      841 tcatggattg ctactgttgt atttggtgtt gtgtttggtg cctggcacca aatacaacag
      901 tgcagtgccg ttgtatttgg tgccaggcac tttttgcaac gccgggcact ttttacaaaa
      961 cgttccaaaa gctttttatg tcagttccgc agtttaatat ataatcaata attgacattc
     1021 cctccgcgaa ttctccccaa agctgactgt acggcttttc ataaaaatct gagtattcca
     1081 gttctatgcc atagtccata aacatttgag gatcttgata tttggtgccg cctctgcctg
     1141 ataaatactt ggtgccgcct acagcttcac atattttaat tattcgcata gtgcttttct
     1201 cttcaatatt aagctgggaa gaaagcatta ttctggtctt tatgttaaga agcccacata
     1261 ttttagtaat tgtattaata ttaaaatcag acaggttatc gctattatat gacaggcagt
     1321 tttgtataaa cggaataaat tcattaaaat atgctgctct cttgtaattg taatgaatat
     1381 ttttaaggtg gttagacctc cagtcctgtt cattatttat gcaaacctca tttattttgc
     1441 accttgagct gtcgttaaat tttgatggta tggtcagcca gtgctcaccc atgtgagttt
     1501 taatcttatt tctatttata tagctgcctt ttatatactg gacatcatcg agaaatataa
     1561 aaatatcact cttatatatt ttgtagaagt atcccatcca tggtatataa ttaggttgat
     1621 gaattgaaac tatcattctt gctaattctc cttatatatt tagccatcta tatttcattg
     1681 taaatatcta atcgtctata ttatccacct gataacttca aaaacctccg catattgcac
     1741 accaatctgt gtccctctgg ttaccgccag tgcttttata aattcggatg aaaagtattt
     1801 ctttccctgc tgagactcat agcatccaag agctctgatt ttattattaa tattttcata
     1861 atctagttca acaaagcatg cagtatttat agtaaggtta ttccatggaa gttcatatcc
     1921 catcacagac ttaaatttga aagctctaac cccttctctg gctataacat ggtgatcctg
     1981 gtgaatgtca tttatgctgg gtaaaaaaat aatatcagga tcaatgcttc tattgagaat
     2041 aaccaaatct tcaagaattt cttgtctaca tttttggaag tttctaacat catagttgta
     2101 aatatgtaaa caatctttgc ttattcccag tatagccgtt gcatttctta cctctgttcc
     2161 caatatatct gagggatatt cctctgatac cgatctttca gcaatggaaa acgcagcata
     2221 atgaacctct gttccactat ttaccaatct atctattgtt ccaccacagc ctaattcacc
     2281 atcatcggta tgtggtgcca acactagtac tcttttaaac aacatatata tacctcacat
     2341 ttcatttata ggggacagtc ctctagcttt agtcctctag ttttactcta gttttagttc
     2401 tccagtttta aagatttata cagttccctc gctgtaatac ctctgccacc atattctttt
     2461 aaatacttca gcactttaat ataaatatct ttccatccag ggtaatctct gtcataaaaa
     2521 acgcccgtat ggaaattaac tgctattgca ccttttacat cccttgtctc atctagaatt
     2581 cttttgattt tttcccaagc tttttcttta tcttttaatg agacattaac gagcgtttga
     2641 tccataatag tcaatggaat ttccagcacc tctatcatcc tgtcttttgc caaatcatac
     2701 ggataaaagg ggaaggccac tcctgctcta aaaccttcac agtcagcata tccaagagta
     2761 gtgtcatatc tcatcccagt ttcttcaatt ttatgccatg ttttgggaac atcaaacctc
     2821 aagtagtgat ttctacagcc atataccatt tttccggtta tctcctcaat tcgtgccttt
     2881 tcttctttaa tctttagaga attattatat gaaaaataat ttgtatgaag ggccgattcc
     2941 caacctgata actcaatttt tttaataata tcaactactt cctctaggtt gtaccgggca
     3001 ccatatcttc cccttctgcc attcataaaa taaaaagtgg acttaaaacc atacttttct
     3061 tctagttcca taaacttctc aaaatccaga taggcatcgt ctcttataaa tatcctttta
     3121 aatgcgaaaa gtatattcct tgtaccttct aagattttcc cggcttttat ttttgcaaga
     3181 ccatttctta ttctatgaag aaatacatat gggtttctat cagttatatt atctacgtca
     3241 tgagttaata caacagtaaa ttctctttct gcaattttca ggttcatgcc tttattatta
     3301 gcagctcttt caagagcttc ttttagtcca tttgcatagt aattaattac tgggatatac
     3361 aaatccttgc gtttgctata ttttgcaaga aatctgcccc tgctgtcctt gagcttttcc
     3421 ccgtattctt cagaacacgt tattacttca agtatttcat ttataacata atcacttatt
     3481 acgacgtcat atttcctttc ataactgtta tttatattac aacttttttt attgcagcct
     3541 tgacctgtgc aattgcagct ttcactaatg gagctaatgc tttcacctat gtaataacca
     3601 ttttttccaa tagaattgct ttcgcaagat atacttatat cattatccat ggatattccg
     3661 gatatcatta aaagcgtttt tataacatat ctgcatttat tccggaaact cagatcttca
     3721 agatgaaaat acaattctat cattttaagc tccaacccct aatcactaaa tattgacctc
     3781 ccactgctat ctccaactcc cgatctccag catctaacct ctcacttccc atttctcgcc
     3841 tctcacttct aacatccaga caaatatttg tatatattca agagcccatt ttcaatgctt
     3901 tcccagttgt gcttttccat cactgcctgc cgaccccgct cacccatttt cagggtctcc
     3961 tcaacatttt caagcatata gtcaatagcc gctgctacct cattttcatc agaagggttt
     4021 actagcaggc cacatctctc gtcacccaca atttccttta taagagggta atctcctgca
     4081 ataaccgcca atccgcaagc catgtattca aacagtttta taggctgaca gtttgcatcc
     4141 acaggatcta caggctggag aagtacaaaa ccaattatgt ttctttttaa taattcaaat
     4201 acatttttat gctcaacaac cccagtgaat tctacagtat tttcacaatt atttttacaa
     4261 ctacccccga taaaaaattt ttgtttttca agagggaagg gtagttgacc attgattccc
     4321 aacaaactgg ctgcagcatt accaaattca cttccagctt ttccaataaa agtcatcctc
     4381 gagttattcc tagacttatt tattgcttct aaaatcttta tacaaccctt atcactgcta
     4441 attccacctg cataaaccaa tccccttaaa tccttacatg ctacttcatc ccctatgaaa
     4501 ggcttaacaa tagaaagcct tgggtagtta ggaagattaa gtacatgctt gttgtatttt
     4561 ttaaattttg ataccaatat cctgtttaca gttattatat aattgcactt tctcgcaaga
     4621 tacccttcaa accaatatac aagtttcttt attacctgct tcgcaatccc tttaaagtgc
     4681 agtgcaaatg tttcaggatg gtattcatga ctgtcataga taagcatgct gtttaatctt
     4741 tttttcagtt tataccctat atacaaagca tcaggttcat ggcagtgata aacgtcggct
     4801 ttagcctcaa tacctttttt atatagctga accaatgtct caatacgatt tttcaagcca
     4861 tgatttttac gtacaggtat ccctataatt ttcaccccgc caatttcatt atattcccta
     4921 tcgctttggg caataatagt tatgtcatta tattcttttt ttaatgtcaa tacctcttta
     4981 taaaaaatcc tgtcatccag aggaccatga ccgcttgtaa gcatgcaaac tctcataccg
     5041 attttcccta tacttccctt ctacataatt ttcttctgca ctatcgcttt cttaactatt
     5101 gccttcttaa ctattacttt ctgcacgatt cccttatgcc tttttaaaat atttatcatg
     5161 gttcttataa gccaggaaca cttgttgatt cttaaccttg gtataagttc tccgccgaaa
     5221 ccccttaaaa aaagctctac acttttaatt gaagaacctt cgaaattcaa ttctcccgac
     5281 acttctgagg caaattttat cgcttcccat accaataaag aaggaattcc cagctttcta
     5341 ccctcttcac tgctgcacat ggcaactcca tgtgcagctt ttctatcata gacaatataa
     5401 tttgctccta gaatattatt atccttatct acagcaaaca gtattttccg tgaatttctt
     5461 tctttcagag cattatcaag attgaccaga aactctttag tataagtaat atttgaattc
     5521 tgacgcatgt aggtcccatt gtcaagttcg taaaagcgat caatgctaaa atcttctgtc
     5581 gttattctta ttccggactt aattgctttt ttgatctttt cttttaccgg tcctttaaac
     5641 tggttgaata tctcattcag cctagctttt cccaattctg tctccagagc tcttaaattt
     5701 cttataacat atgcgtactc tatatcaaat ctaaactttt cccatttaaa tggaaggcaa
     5761 tttatatagt tttcgctgaa cacatagtca aaaagcaggt aagaaggaag tttttctatt
     5821 aaagcacaca tgacacttat ctcttttgag attttattca ccggcttcat tacagcaaag
     5881 tcatttaata aaataccaag ggttggtgta agtgaagggt ttctatagaa tcttctatat
     5941 attgaa
//
LOCUS       DAOSDD010000256         5865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4861_length_5865_cov_5.370052, whole genome shotgun sequence.
ACCESSION   DAOSDD010000256 DAOSDD010000000
VERSION     DAOSDD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_5865_cov_5.370052"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..812)
                     /locus_tag="PK733_16795"
     CDS             complement(318..812)
                     /locus_tag="PK733_16795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ12228.1"
                     /translation="MSFSFLNNFVKMIPSNVDIVGITVKEIIQYLEAVCGYIDDDVLF
                     DLRVILNELILNAIIHGNKADMNKYVKITTGLAKGNYIYMVVTDDGEGYDYEYLLKKC
                     EILKKCEVPYDDYNINYLSETGRGILIIKSLCEKVKFNEKGNKVVVLKKLNGIGETAD
                     NCLK"
     gene            complement(1484..2245)
                     /locus_tag="PK733_16800"
     CDS             complement(1484..2245)
                     /locus_tag="PK733_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ12229.1"
                     /translation="MLRIETDVVEASVINEQELFSIYNKNPSVKNRNAIVGKYLYLAE
                     IISKKFVNRGVDYDDIFQVASIALIKAVERYKPERGVKFVSFATPTIVGEIKRFFRDK
                     ASVIRIPRRIYEDYRKVNMAKEYLTTKLQRVPQVDEIAEYLKISEETVLEIIESQNVH
                     NIKSFDQSAYTDDDIELYETIGDEDRSFERIENLDFLRRSIDKFSDVEKKFIQMRYFE
                     NNTQKTIAEELGVSQMYISRMERKVLGKFRTILNK"
     gene            complement(2248..2640)
                     /locus_tag="PK733_16805"
     CDS             complement(2248..2640)
                     /locus_tag="PK733_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ12230.1"
                     /translation="METTDIIELSLPFKPEYVSIARLTSSAVASRIGFDIETVEDIKV
                     AVSEVCTKYIKKGSNCANHYKLIFKVFKDCLEISFDCQDKTLKCIFSQEDDGLAISII
                     NALMDEVKLCSEDENYLLNMSKKLEGKV"
     gene            complement(2645..2962)
                     /locus_tag="PK733_16810"
     CDS             complement(2645..2962)
                     /locus_tag="PK733_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ12231.1"
                     /translation="MSEDLVIKNECLNDKVIVILSGEADIYTSSQLKDKLYELIDKNK
                     TDIIIDCNELSYIDSTGLGVFAGALKKAGQYNKSIYIRGLKSNIKKLFTITGLDRLFI
                     IEE"
     gene            complement(3086..3940)
                     /locus_tag="PK733_16815"
     CDS             complement(3086..3940)
                     /locus_tag="PK733_16815"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ12232.1"
                     /translation="MQKTDAYEILGLKSGASKVEIEKRYSIILRKYKSSDNEQSSDIN
                     FDEVTEAYNLLMGYTIEETEEERIRRKPNPVLEKMGIDQARQDKLLNNLYYFRIHIVV
                     GIILLVTIILSVKSCVNRVIPNLNIVFMGDIYCSDTENLEKTLKENIPEIEAVGIENI
                     LISNAQEKQDPQIQMAMLQKAAVLMAAGDIDVYILDKVNFEKFGEQGAFMKLEDMAKQ
                     FRIPDKKLRKCQIKEEDEKEFIYGFDIKDNKILKDASTSGKEMIAVISIKAKNYDNAV
                     KLMELLVQ"
     gene            complement(4047..5540)
                     /gene="lysS"
                     /locus_tag="PK733_16820"
     CDS             complement(4047..5540)
                     /gene="lysS"
                     /locus_tag="PK733_16820"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOJ12233.1"
                     /translation="MSDDILNTNDTIDLNEILRVRRQKLQELQANNKDPFRKVKYEVN
                     SSSLEVIDNFENMEGQLVSLAGRIISKREMGKASFCDIQDKYGKIQLYVRVNEIGEES
                     YEDFKKYDIGDIIGVEGEVFKTRKGEISIKAHKIILLAKSLQPLPEKWHGLKDVDLRY
                     RQRYVDLIVNPEVKDTFITRTKIIKAIRKYLDDRGFLEVETPLLNPIAGGAAARPFIT
                     HHNTLDMDLYLRIAPELYLKRLIVGGLERVYEIGRMFRNEGISVKHNPEFTMMEVYQA
                     YTDYRGMMELAEGIVSTVAQEALGTTKIEYQGQEVDLTPPWDRLTMIEAVKKYTGVDF
                     DEISGDEEARKIAIEKGIEIKADIKWGEVLCALFEEYVEEKLIRPTFIYDYPIEVSPL
                     TKKKPDRPELTERFEIFVTGREMGNAYSELNDPIDQRQRFVEQMKKREAGDEEANMID
                     EDFLNALEYGMPPTGGLGIGIDRLIMLLTDSWSIRDVLLFPTMKPKA"
     gene            complement(5673..>5865)
                     /locus_tag="PK733_16825"
     CDS             complement(5673..>5865)
                     /locus_tag="PK733_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOJ12234.1"
                     /translation="LDIEMDEEVEYFIVGSTEADPGKCKISNESPVGRALIGKSKDDI
                     VEVFVPAGTIKLKILNIDK"
BASE COUNT         1751 a         1170 c          721 g         2223 t
ORIGIN      
        1 gctttttaag ccttttaagc tatttaagct tcttatctct tcccacttac ctagcttttc
       61 tgttaaaaac cagttgcctg agtaggtttc ttaaataggc cgtttaaatt agctgcttat
      121 tgatgttgct cactcacaag cggcttatac aatataccat tatatgcatc ataatatcaa
      181 gatgcacaca actttattta ccttaattaa agtaatttag tcttatattt ctacatcaat
      241 ctacataagt tatataatta tatcaggata tctttgcttt aaattcactt ttcgacattt
      301 ttgctttacg ataaatatca ctttaaacag ttgtcagctg tttcgccaat accgtttaat
      361 ttctttagta ctacaacctt gtttcccttt tcgttaaatt tgaccttttc gcaaagactt
      421 ttgataataa gtatacctct gcctgtttca cttagataat taatgttata gtcatcataa
      481 gggacttcac attttttcag aatctcacat ttttttaaaa gatattcata atcataacct
      541 tcaccatcat ctgtaactac catatatata taattccctt tagccaggcc tgtagttatt
      601 ttaacatatt tattcatatc ggctttattg ccatggataa tagcatttaa aataagctca
      661 ttcagaataa ctcttaaatc aaaaagaaca tcatcgtcta tatatccgca aacagcttca
      721 agatactgaa tgatttcttt gacagtaatt cctacaatat caacattact agggatcatt
      781 tttacaaaat tatttaaaaa actaaagctc aagactactc ccgtcccatc tttaccactt
      841 tatgtattcc accaatattt cgtttaatag ttatatctac ctataaagta tattaacatg
      901 cgttaattta attacagtgt atttgcatct tagttgcatt tttatattat tactaataat
      961 ttttaatcta tacggtacaa ttatttatac ccctattgtt tttatagtaa acagaattta
     1021 gcagcaagaa gcgtatttta ttctgtagtg aatattaggt gcatttaatt tatctgaaga
     1081 aaatattggt aaggggcata agtggaggtg ctgtagctgg gcaggggaaa ctcttcactc
     1141 aagtaacaga tattcctttt atgttgaaga gtttccccgt acctgagcga aggaatagca
     1201 aaatcagtga ttgaacagaa caattacaat tcttttgttc ggattatttt gtgagtttga
     1261 ttttgcccgt agctgaattt aaaggctcta tgcaccgtaa cggtagcccc tggtaaatat
     1321 ttcttcagat atgataaatg cacccgtgaa tattgtacac tgaaatcgat tattatcatt
     1381 taacgggaag tgtgcattaa ggtaatttat actttcctac tagttataga aaatgcgccc
     1441 ggtaacccgt gcgcattatt attcattatt tattatttat tatttattta ttaaggatag
     1501 ttctgaactt gccaagtact tttctttcca tacgggatat atacatctga gatacgccca
     1561 actcttctgc aatagttttt tgcgtattat tttcaaaata tctcatttga ataaattttt
     1621 tttccacatc gctaaactta tctatacttc tccttaagaa atccagattt tctattcttt
     1681 caaatgaacg gtcttcatct cctattgttt cgtataattc aatgtcatca tcagtatatg
     1741 cactctggtc aaaagatttt atattatgta cattttggga ttcaatgatt tcaagaacag
     1801 tttcttcact tatttttaaa tactcagcta tttcatctac ttgtggaact ctctgcagtt
     1861 ttgtggtcag gtactctttt gccatgttta ctttacggta gtcctcataa attcttctcg
     1921 gaattcttat aaccgatgct ttatcacgaa aaaacctttt tatttcacct acaattgtgg
     1981 gagtggcaaa acttacaaat ttaacacctc tttcgggttt ataacgttct acagctttaa
     2041 ttaatgcaat agacgctact tgaaatatat catcataatc tactccccta tttacaaatt
     2101 tctttgatat aatttccgcc aaatagagat acttacccac aatagcattc ctgtttttta
     2161 cagaagggtt cttattatat atactaaaca attcctgttc attaataaca gaagcttcta
     2221 ctacatcggt ttctatccgc agcattatta tactttcccc tcaagttttt ttgacatatt
     2281 taaaagatag ttctcatctt ccgagcataa tttaacctca tccattagtg catttattat
     2341 agatattgcc agtccatcat cctcctgact aaaaatacac tttagtgttt tatcctgaca
     2401 atcaaacgaa atttccagac aatccttgaa aactttgaaa ataagcttat aatgattagc
     2461 gcaattactt ccttttttta tatatttagt gcaaacttct gaaactgcaa cttttatgtc
     2521 ttcaactgtt tcaatatcaa aacctattct gcttgctacg gctgaagaag tcagccttgc
     2581 tatactaaca tattctggtt tgaaaggcag cgacagctca ataatatctg ttgtttccat
     2641 atcttcactc ctcaatgata aacaatctat caagtccggt gatagtaaat agtttcttta
     2701 tattactttt aagccctcta atatatatgc ttttattata ttgtccggct tttttcaggg
     2761 cacctgcaaa aacaccaagt cctgtactat caatataact taattcgtta caatctatta
     2821 ttatatcagt tttatttttg tcaattagct catataattt atcttttaat tgtgatgatg
     2881 tataaatatc cgcttcacct gacaatataa ctattacttt atcatttaag cattcatttt
     2941 taataactaa atcttcgctc atatggtcac tccttactta acatccctgt aataaaatac
     3001 acgagttaat acccattctg ttaatggccg ttcatttata aattcatttg taaaaacgtt
     3061 gaaaggtatt tttcatgtgt tatttctatt gtacaagcaa ctccattagc ttgacagcat
     3121 tatcataatt ttttgcttta atgctaataa cagctatcat ttccttacct gaagtacttg
     3181 catcctttaa tatcttattg tccttaatat caaaaccata aatgaattcc ttttcatctt
     3241 cttcttttat ttggcattta cgaagtttct tatcaggaat acgaaactgt ttagccatat
     3301 cctccaactt cataaatgcg ccctgttcac caaacttttc aaagttcact ttatccagaa
     3361 tatatacatc aatatcccct gcagccatta aaacagccgc tttttgcagc attgccattt
     3421 gaatctgagg atcttgtttt tcctgagcat tggatattaa tatattctcg attcctactg
     3481 cttcaatttc aggaatattt tcttttagtg ttttctcaag attctcagta tcactgcaat
     3541 atatatctcc cataaagaca atattcaaat ttggtatcac ccggttaaca caacttttaa
     3601 cagaaagtat aatggtaaca agcagaataa tgcctactac tatatgaatt ctaaagtaat
     3661 aaagattatt taaaagttta tcttgtctgg cttgatctat tcccattttt tccaatacag
     3721 ggttaggttt tctgcgtatt ctttcttctt cagtttcttc aatggtataa cccattaaaa
     3781 ggttatatgc ctctgttact tcatcaaaat taatatcaga tgattgctca ttatcacttg
     3841 acttgtattt tcttaaaata atagagtatc ttttttctat ttctactttt gatgctccac
     3901 tttttaaacc aaggatttca tatgcatctg ttttctgcat ttataccacc cctttactta
     3961 atgcaaccat atatcgtatt tcatatatta taattactgt tgctaacaaa ataagtaaaa
     4021 aagaaaaata ctttctaaac tttaagttat gctttaggtt tcatagtagg aaatagcagt
     4081 acatccctta ttgaccatga atctgtaaga agcattatta atctgtcaat gcctattcct
     4141 aatcctcccg tcggtggcat gccatattca agagcattaa ggaaatcctc atctatcata
     4201 tttgcttcct catctccagc ttcccttttt ttcatctgtt caacaaatct ctgcctttga
     4261 tctatagggt catttaactc tgaatatgca tttcccattt ctcttcctgt aacaaatatt
     4321 tcaaatcttt ctgtcaattc aggcctgtcc ggcttttttt tggtaagtgg agaaacttca
     4381 attgggtaat catagataaa tgtaggacga ataagttttt cttcaacata ttcttcaaat
     4441 aaggcgcaaa gtacctctcc ccattttata tcagctttta tttcaattcc tttttctatt
     4501 gctatttttc tggcttcttc atcccctgaa atttcatcaa aatccacacc tgtgtatttt
     4561 ttaactgctt ctatcatcgt aagcctgtcc catggaggag taaggtcaac ttcctggcct
     4621 tgatactcga ttttcgtcgt tccaagtgct tcctgggcca ccgtggatac aattccttca
     4681 gctagctcca tcatgcccct ataatccgta tatgcctggt agacctccat catagtaaac
     4741 tccggattat gcttcacaga tataccctca tttctgaaca tcctgcctat ttcatatact
     4801 ctttcaagac ccccaacaat aagccttttt aaataaagct ctggggctat tcttagatac
     4861 aaatccatat ccaatgtatt atggtgagtt ataaacggcc ttgcagcagc tcctcctgca
     4921 ataggattca acaaaggagt ttcaacttca aggaaccctc tgtcatcaag atattttcta
     4981 attgctttaa taatttttgt ccttgttata aatgtgtcct taacttcagg attaacaata
     5041 agatcaacat atctttgtct atatcttaaa tcaacatctt ttaatccatg ccacttttca
     5101 ggcaatggct gcaacgactt tgcaagtaaa attatcttat gggccttgat agatatctca
     5161 ccttttctgg ttttaaaaac ttccccttca acgcctataa tatctcctat atcatacttt
     5221 ttaaagtctt cgtaactctc ttccccgatt tcgtttactc ttacataaag ttgaattttc
     5281 ccatatttgt cctgaatatc gcaaaaagaa gctttaccca tttccctttt agatataatt
     5341 ctacctgcca gggatactaa ctgaccttcc atattctcaa aattatcaat tacttcaaga
     5401 ctggaactat ttacttcata tttaactttt ctaaaaggat ctttattatt ggcctgaagc
     5461 tcctgcaact tttgtcttct aacccttagt atttcattta gatctatagt atcatttgta
     5521 tttaatatat cgtcagacat atttatcctc cttaagactt aaaaagccat tatatattat
     5581 attatatata atgaaaagtc gtatttcaat taataaaatc ctaaatacct ataatagctg
     5641 caacggctac aaaagttaca aagctataat atctatttat ctatatttaa tattttaagt
     5701 ttaattgtac ctgcaggcac aaatacttct actatatcat cttttgattt tccaataagt
     5761 gcccttccta ccggtgattc attggatatt ttacatttac cagggtcagc ttccgtagag
     5821 cccacaataa aatattcaac ttcttcatcc atttctatat ccagc
//
LOCUS       DAOSDD010000257         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4871_length_5854_cov_6.041386, whole genome shotgun sequence.
ACCESSION   DAOSDD010000257 DAOSDD010000000
VERSION     DAOSDD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4871_length_5854_cov_6.041386"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK733_16830"
     CDS             <1..708
                     /locus_tag="PK733_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ12235.1"
                     /translation="DNGVLNGITFAAEKGKVTAIVGPSGSGKSTIIKLLLRLYETDKG
                     QISFGGIDIRNFKHEDWRSKISYVSQDASLFSGSIKENILYGKVGADMNEVKNAAAAA
                     CIDEFIEKSEKGYDSLIGEKGVKLSGGERQRISIARAVISNPEILVLDEPTSSLDNEN
                     EHMVLEALINLMTGRTTIVVAHRLSTIKNADQIVYIEDGIVAESGTHDELLDIKGKYY
                     NMYSSMINSEVERDVAI"
     gene            705..2690
                     /locus_tag="PK733_16835"
     CDS             705..2690
                     /locus_tag="PK733_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12236.1"
                     /translation="MKIPSQKRCSQKRCGRKISSQKRCSHTKSGLRTVTRIMSFAGPY
                     KKLLYLGFFLLIPDVVFSVGIVVAIQNIVNVMAEQQIGRLSYLLIVNLIIVIACVILS
                     MLGTALRKYASFQINKRMTDMLLKHVNKLPFSYIQDNHTGDLVERVNTDVGKAVSAVN
                     DDGIVRLVTNLLVSISAFVYLSNIDLKLTLLAVATGPVTFLSGRFFDKKIRKTSESLN
                     KKSGELRGIFQEFLQSMPIVRTLALDTVFSDKFMLKKKEQMALTRKKAVLNNLMWRIV
                     VTVNFSVTILLTYYIAMESMKVNMSIGSALSFVFLLGRLQWPFVNISSTWGTIQNALG
                     AADRIFTILDMDTEDIMADGGLAEKEEEDGNHLLKKDYGHISCEDISNNCQEVLDIVN
                     MSFSYNNKPVFTELNFSVKAGETVAIVGASGSGKTTLARLCCGLYMPDRGDVRVFGYS
                     TRNNLNEARNMLAYVSQVPYIFSGTVKENILLGLKQSSIIGSGEYDEKDNMDNYIGVS
                     SGQRVENSIDEIHNNTSIDDMAYASAAMANADSFINAMENGFNTVLGEKGSNISGGQK
                     QRITIARALIRNAPLIIFDEATSSLDNESEMMIRNTIDNLIGKKTMLIIAHRLSTVQN
                     ASRIIVLDGGKIVEEGTHNQLMETKGAYYKLYNEGAA"
     gene            complement(2834..3826)
                     /locus_tag="PK733_16840"
     CDS             complement(2834..3826)
                     /locus_tag="PK733_16840"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOJ12237.1"
                     /translation="MLSFSRETIVDKNTIADKSIKKVTIIGAGFVGATTAYTLMLSGL
                     ISEIAIIDVNMEKVEGEVMDLNHGMPFVRPVKIYEGQYEDCRDSDIIIITAGANQKPG
                     ETRIDLVKKNTQVFKNIIPQILNYYNDSILLVVTNPVDILTYVTCKVSGLPKNRVIGS
                     GTVLDTARLRYLIADHVGIDARNVHGYIIGEHGDSELAAWSIANIAGISVDEYCNKCQ
                     KCDKKLSQIDIFKDVKNAAYEIIEKKGATYYAVALAVRRIVEAITRDENSILTVSSLV
                     EGQYGINDIALSLPSIVNSNGICTILDIPLNTEEYKLLVKCSNSLKEVVKTLDI"
     gene            complement(4108..4431)
                     /locus_tag="PK733_16845"
     CDS             complement(4108..4431)
                     /locus_tag="PK733_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12238.1"
                     /translation="MNEDYYRETTASFVSYFDEQLSLLRNHALTIAYGNIEANNKIIK
                     ETIESHPYYYLTATKVLADYKIGLPNTTDIGLYFKSTDYIITSKFKYSINDFLSEINS
                     YKYKL"
     gene            4984..5454
                     /locus_tag="PK733_16850"
     CDS             4984..5454
                     /locus_tag="PK733_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12239.1"
                     /translation="MHQAILAMAAVVLDEEGTTQEMIDYDFQTGKLDMIIGAEYPYGR
                     KYILTGSNILTTLVDDVDRGGFGNESAPGYNNLWLNQKKVVTDVFDGYDRYADVDLYE
                     NVKFKKMFQAGYPPLNYIAKQADNPVLSRVVCSFQCSSLAVFYMSCFSVITIVQ"
     gene            5557..5697
                     /locus_tag="PK733_16855"
     CDS             5557..5697
                     /locus_tag="PK733_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12240.1"
                     /translation="MISITDLMKSLNVEKYPERWNEIYYNAMKEYEYYGAFLLTRNIL
                     QS"
BASE COUNT         1945 a          902 c         1136 g         1871 t
ORIGIN      
        1 gataacggag ttttaaatgg aattacattt gctgcagaaa agggaaaagt tactgctatt
       61 gttgggccca gcggctcagg aaaaagcaca atcattaaac tattgctaag gctttatgaa
      121 acagataaag gacaaattag ctttggtggt atagatatca ggaattttaa acacgaagat
      181 tggaggtcaa aaatatcata tgtatcccag gatgccagct tgttttcagg ttcaataaag
      241 gagaatatac tatatggcaa agttggtgca gatatgaacg aagttaaaaa tgcagcagct
      301 gctgcctgta tagatgaatt tattgaaaaa agcgaaaagg gttatgatag cttgattggt
      361 gaaaagggtg taaaactctc cggtggggaa cggcaaagga tatcaatagc aagagctgtc
      421 atttcaaatc ctgaaatact tgttttggat gaacctacat cttctttaga taatgaaaat
      481 gaacatatgg tattggaagc attgatcaac ctgatgacag gaaggactac tattgttgta
      541 gcccaccgcc tttctacaat aaagaatgca gaccagattg tgtatattga agatggtatt
      601 gttgcagaat ccggaacaca tgatgaatta ttggatatta aagggaagta ttacaatatg
      661 tatagcagta tgataaatag tgaggtagaa agggatgtag ccatatgaag atacccagcc
      721 agaagagatg tagtcagaag agatgtggcc ggaagatatc cagccagaag agatgcagtc
      781 atacgaagag tggcttaagg acagtaacgc ggataatgag ttttgccggg ccatataaaa
      841 agttgttata cttaggcttc tttctattaa ttcctgatgt tgtatttagt gtaggtattg
      901 ttgtagcaat tcagaatata gtcaatgtaa tggctgaaca acagataggc agattaagtt
      961 atttgctgat agtaaatctg ataattgtta ttgcctgcgt aattctttct atgctgggta
     1021 cagcccttag aaaatatgca tcttttcaga taaacaaacg aatgactgac atgctgctaa
     1081 aacatgtaaa caagctccct tttagctata ttcaagataa tcatacagga gacctggtcg
     1141 aaagagttaa taccgatgtg gggaaagctg tcagtgctgt caatgatgat ggcatagtaa
     1201 gattggtaac gaatctttta gtaagtattt cagcatttgt atatttgtca aatattgacc
     1261 ttaagcttac attgctggca gttgcaacag gtcctgtaac atttttatca ggacggtttt
     1321 ttgataaaaa gatacgtaaa acttcagaga gcttaaataa aaagagcgga gagcttagag
     1381 ggatattcca ggagttttta cagtcaatgc ccatagtcag gaccttggca cttgatacgg
     1441 tgttttctga taaatttatg ctaaagaaaa aggagcaaat ggctttaacc agaaaaaaag
     1501 cagtattgaa caatttaatg tggcgaattg ttgtaacagt aaatttttca gtcactattt
     1561 tattaaccta ttatatagca atggagtcta tgaaagttaa tatgtccata ggttctgcac
     1621 tttcttttgt ttttctttta ggcagactgc agtggccatt tgtaaacatc tcaagcacat
     1681 gggggactat acaaaatgct cttggagcag ctgacagaat attcaccata cttgacatgg
     1741 acactgagga tattatggca gatggagggc tggcggagaa agaggaagaa gatggaaatc
     1801 atttgctaaa aaaagattac ggccatattt cctgtgaaga tatatcaaat aactgtcagg
     1861 aagttcttga tattgtaaat atgagttttt catataataa taaacccgtt tttacagagt
     1921 tgaactttag cgttaaagcc ggtgaaacag tggctattgt aggtgcaagc ggttcaggta
     1981 aaactacatt ggccaggctc tgctgcgggt tatatatgcc ggataggggt gatgtgagag
     2041 tctttggtta ttccacaaga aataatctaa atgaagccag gaatatgctt gcctatgttt
     2101 cacaggtacc gtacattttc tctggtactg ttaaagagaa tatattgctt gggttaaagc
     2161 aaagcagtat tataggaagt ggagaatacg atgaaaaaga taatatggat aactatatcg
     2221 gagttagcag cgggcaaaga gtagaaaata gtattgatga aatacataac aataccagca
     2281 ttgacgatat ggcatatgcg tcagcagcca tggcaaatgc cgatagtttc ataaatgcta
     2341 tggagaatgg tttcaatacg gttctaggtg aaaaaggctc aaatatctca ggaggacaaa
     2401 aacagcgtat taccattgca agggctctta tacggaatgc accacttatt atctttgatg
     2461 aagcaacatc atccctggat aatgagtcag agatgatgat tagaaatact attgacaacc
     2521 ttattggaaa gaaaaccatg ttaattatag ctcacaggct ttcaacagta caaaacgcat
     2581 caaggattat tgtccttgat ggcggcaaaa ttgttgagga aggtacccat aaccagctga
     2641 tggagactaa aggggcatat tacaagttat ataatgaggg agcggcgtga aggcgcatta
     2701 taaatttttg aggaaatata ttccggaagc taacgttacg gtacttagag ccttcactaa
     2761 tgcttcttcc tttaatctct tcagaaataa atatgcacca ctgaaactga agtacttcac
     2821 aggctgaatc atcttatata tcaagtgttt tcactacttc ttttaaggaa tttgagcact
     2881 taaccagcag tttgtattcc tcagtgttta agggtatatc caatattgtg cagataccat
     2941 tgctattaac aatagatgga agactcaaag caatatcgtt tattccatac tgcccttcta
     3001 caaggcttga tactgtgaga atagagtttt cgtctcttgt tatggcctca actattcttc
     3061 ttactgctag tgcaacagca taatatgttg cacctttttt ttcaattatt tcataagctg
     3121 cattctttac atccttaaaa atatctatct gcgatagttt cttatcacac ttttgacatt
     3181 tattgcagta ctcatcaaca gaaatacctg caatatttgc aatgctccag gcagctaatt
     3241 ccgaatctcc atgctcccca ataatgtatc catggacatt tcttgcatca attcctacat
     3301 gatctgcaat aagatatctt aaacgtgcag tgtcaaggac ggtgcctgac ccaataaccc
     3361 tgttttttgg caaacctgaa actttacatg ttacataagt aagaatatct acaggattgg
     3421 taacaaccag taaaatcgag tcattatagt aatttaaaat ttgggggatt atatttttaa
     3481 aaacctgtgt attcttttta accaggtcta ttctggtctc accgggtttt tgatttgctc
     3541 ctgctgtaat tattattata tcagagtcac ggcaatcctc atattgccct tcatatattt
     3601 ttaccggtct tacaaaaggc atgccatgat ttaagtccat gacttctcct tcaacttttt
     3661 ccatattgac atcaataatg gcaatttcag agataagacc gcttagcatt aatgtatagg
     3721 cagtggttgc acctacaaaa cctgcaccta taattgtaac ctttttaata gacttatcag
     3781 ctatagtgtt cttatcaact atagtttccc tgctaaaaga taacatttta ctatttgttg
     3841 tagttttatt acaaaaatcc atttctttac tcctgacttt aatgaatatg caaaaaacaa
     3901 attgctcctt gtaaattatt tttgcattat atatagttat tatgctatag aaacttagta
     3961 aatatatatc caaaaacaag ggtgcattta ttttatatga tgaaaatgtt ggtaaggggc
     4021 attagtgaag gctctaagca ccgtaacgat agcacctggt aaatgtttct ttagacttga
     4081 ttaatgtacc cgtaaaacta tttaacatta tagtttatat ttataactgt tgatttctga
     4141 taaaaaatca ttaattgagt acttaaattt tgaggttatt atgtagtctg tacttttaaa
     4201 atataaacct atatcggttg tattaggcaa gcctatttta tagtctgcca ggacttttgt
     4261 tgctgtgaga taataatatg gatgtgattc aatagtttct tttataattt tattgttagc
     4321 ttcgatattt ccataagcaa tagtaagagc atggtttcgc agtaaactaa gctgttcatc
     4381 aaaatatgaa acaaaggatg cagtagtctc ccgataatag tcctcattca ttttcagcat
     4441 ctcgttatat gaacgacgga taacagtaaa accaaaaata agccctggaa tataaagtgc
     4501 caggcacaag ctcgatacaa accgtagtaa gtagttgtac ttaactaaga agtttttgtt
     4561 tttatagaca tatctgacac agcctttcga tgcactattc ttcaatcggt tgatttaatt
     4621 ataccttaat agctatttta tgcaaactgt aattttgtgc agacgacctg catttttcta
     4681 gcaaactctg aaaaaatatc tgaaaaaata caggaagatt tggagtataa gcggcttaca
     4741 catttttacg ggtgcttagc acaaagctac agtttacatt tccaggttat aattataaaa
     4801 tgatttcaca agcaatagat gattatatgc taattattta tgcgtttatc aaatgtttat
     4861 taaaatttta ttctataact gcatgaataa tcaagagaaa ggatttgatt atggaattgg
     4921 gaacaactaa tataaataca aaaaacgaaa tttcatataa gaagaacctg aggtaatttt
     4981 ggaatgcatc aagcaattct tgcaatggct gctgttgtac tagatgaaga aggtacaact
     5041 caggagatga tagattatga tttccagact ggtaagcttg atatgataat tggtgctgaa
     5101 tacccctatg gaaggaagta tattctaaca ggaagcaata ttctcactac tttggtagac
     5161 gatgttgata gaggcgggtt tggaaatgaa tcagcaccag ggtataacaa tctatggcta
     5221 aaccagaaaa aggtagtaac agatgtgttt gatggttatg acagatacgc tgatgtcgat
     5281 ttatatgaaa acgtaaagtt taagaaaatg tttcaagcag gctatccgcc tcttaattat
     5341 atcgcaaaac aagccgataa tccagtgctt tcaagggttg tatgcagttt tcaatgttca
     5401 agtttagcag ttttttacat gagttgtttc agtgtaatca caattgtaca ataaacaaat
     5461 atttttttat aagttttgct tattataagc cttctacagg ttgtatttta catacatttt
     5521 tttaatatta caaataacac atggtaggtg attaatatga tttctattac agatttgatg
     5581 aaatccttga atgttgagaa atatcctgaa agatggaatg aaatctatta taatgcaatg
     5641 aaggaatatg aatattatgg cgcttttttg ctgacgagga atatattaca aagttaaatc
     5701 gtgaattctg tttattcgat gattggtttg aaatcatctt aatagctgcg aagcaggtca
     5761 gagaaaatgc taaccttatg agatacatat atcttttaaa tatggaaaac caggttttca
     5821 ttttagttat ttaagttggt ttcagcttta tata
//
LOCUS       DAOSDD010000258         5829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4897_length_5829_cov_6.270003, whole genome shotgun sequence.
ACCESSION   DAOSDD010000258 DAOSDD010000000
VERSION     DAOSDD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4897_length_5829_cov_6.270003"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PK733_16860"
     CDS             <1..1204
                     /locus_tag="PK733_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ12241.1"
                     /translation="RNADSQLLGEWLLLSSYRCFPHLNEMTAVLKQAAVLLGDSCSQV
                     ILPDALWCFGNYSPLTVFYTTPGEADCEAKALEKYIAIYSRLTNGHGSGTDALFRAEL
                     AYQRGDLNDAEILAYKASFFAESKQQSVVQLGAAILLAHIALHKADIAGWQRAINSME
                     RAASFEYQDTFVIRSLLDIIRGVLLNEFQNQTSIADWLKNADFGGNRLLPDMINSALY
                     VHLSFLMHQGEFARVIGTLQAVRPADGLLSPYADLFACFPMAISHMALGNHVRAAAFV
                     EHAARRALPDGLVSPFAAFSRLLHGLVEKVIERDHPESLDKFRAVRKRFTEGWVTLRE
                     ALFQGELPPDLTEREYEVARLAAQGLRNSEIAEKLVITESTVRTHLRTIFQKLQIDRR
                     AKLAEKLK"
     gene            1332..1568
                     /locus_tag="PK733_16865"
     CDS             1332..1568
                     /locus_tag="PK733_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOJ12242.1"
                     /translation="MNYIKTVLPIKDYRLLLEMEGGSIVIVDLSAKLNTMKYSELAND
                     KFFKTAVTDGDYVIWGGGRLRLTVNELMEVVLLG"
     gene            1660..>5829
                     /locus_tag="PK733_16870"
     CDS             1660..>5829
                     /locus_tag="PK733_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12243.1"
                     /translation="MYNSVLKRMASVLIIIVMLIPQITAAAVDGTSSKVRYSNAAQYA
                     STGKTNCYPDMGRWLNSEVFEIVPSDKGDEIKPKDINSDFEYSWLMPLPDVKDQINDG
                     LRYDIALDIFGHIFDFDMAAVYVDFMVPIGEGGGARTFITDQYLLEDIDLWKHRSFMG
                     YIPEDATYMRLRLASKKETGFLADKDAAIFYRNIEVYITDDILPVPIGVEYGSRYELG
                     MAAAGKYKDYYGIGNNVYIDILFNEPIFTNDPNYLAYIRNMNHSDRDSFAKNQLAKKY
                     PKDSLVLQHVLNNDGKTTERQFGELKLKFKYKNSDGADKTGYAVAVDRSLYDIDEIYR
                     YDCSKKIKFQYTVQDGDEFQADDIYDLELVGGIITDNSFNMMPDGRRKINFDTSSDNN
                     INAVYRSYAQNFRVETTPPVLLDINGKTPDGEINPGSELEIYFKFSEPVYIVLPDGVG
                     AHRASVNLNSQYIDEYFEVSNAYGSPRAYYLGGSGTNMLKFRYNVKHNKFDPLEIVST
                     GFMGTDTDDDNRGNPYGEVYVSDSAGNMAPLLQNNSLKLSDNKLYVADKIPPEFSAQV
                     IKAEPGEDGFYLEIDASDTGSGLDYDSLMFGFGGLDVSISNLQNNMKPLIPGKKYHSM
                     ELAELFGIDRIDISKLDSFRVIASGKDKKNNYYFSPSSGYIWEIVLSIDTLPPILSRY
                     SINKDAYSDKKGSFILWIKKGDGTSVPAGADSVLAVEYKWVTAGTNPDMEEWIPVNSV
                     YHQGMGNYIAEGPGPSYNIYGDADLYAKAVDKQGNEAVFYIPKALTYEEITKALFINQ
                     LCDMDISLGRTEGRLYVASVRIRNKIGTVIPFKGLWYCMTKEANMPELSDKTGIWKYK
                     EGDEVRTYDYFGDKWDKINGFWYMHICAMDNNDKPICSATVPEALLFDFEAPNISVDE
                     EKQALDDKLQKPYVYAVSDRKYNENIYTNEDFAELEVYTSGNEFSYSLDGENWSTWLP
                     LASKGTFTQMDSDRYYDLHNNNGYNVYKAYVPLEQKEGNMTFYTRYRREGIQSEPVAA
                     SVIRDVTPPTALVNYNPSYTTDSSVIASLSSISDNLCPGEVVKVLGERSHTFYENGTY
                     SFIIEDAAGNKAKVKADVSNIYIEPPDTSKPVISITGTPGGDITNKAECTIKVTDNSP
                     VSIKYVWTFERNWKDVQSSEWQTISNGSTVSKTDELAANVALTWYLHVLASDSSGNTE
                     VASRSYIIDSVAPVISINPNGSENVVESVSTLITIDDALNTEGTYIWHSSDELLPDDD
                     DGWKDVPDNGVVSLSGVDGIWYLYVKAVDAAGNVGTFKTNPFNMVSEYIEPYVVYSGE
                     EKGKIRAFLVSQCPITVDGPVYCDFERDLERENPREAYTVSYDFSYSYGGEEKHTLTA
                     QWDFPEIPSYIE"
BASE COUNT         1826 a         1009 c         1356 g         1638 t
ORIGIN      
        1 tcggaacgcc gattcgcagc tgctaggcga gtggctgctt ctttcttcat atagatgttt
       61 cccccaccta aatgagatga cagctgttct aaagcaggct gccgtacttc ttggagacag
      121 ttgctcgcag gtaatccttc ctgatgcgct ctggtgcttc ggcaactatt ccccgttgac
      181 tgtgttttac actacacccg gtgaagccga ttgtgaggcg aaggcgctgg agaagtacat
      241 cgctatctat tcgcgtctta cgaatggtca cggcagcggt acagacgcac tgtttcgtgc
      301 ggaactggcc tatcagcgcg gagatttaaa tgatgcggag atacttgctt acaaggcatc
      361 attttttgct gagagcaagc agcaaagtgt cgttcaatta ggtgccgcca tactactggc
      421 ccatattgcg ctgcacaagg cagatatagc cggctggcag cgtgccatca attcaatgga
      481 acgagctgct tccttcgaat accaggatac attcgtcatt cggtcacttc tggacattat
      541 ccgcggggtg cttctaaacg aatttcaaaa tcagacaagt attgcagatt ggttgaaaaa
      601 tgctgatttt ggggggaacc gcctgctacc ggatatgata aacagtgcat tatacgtaca
      661 cttaagcttt ctcatgcatc aaggtgagtt tgcaagggtg ataggaacat tacaggctgt
      721 acggcctgca gatggactat tgagtcctta tgcggatctt tttgcatgtt tccctatggc
      781 catcagccat atggcgttgg gcaatcatgt acgggctgct gcatttgtag aacatgccgc
      841 ccgaagagct cttcctgacg ggctggtttc tccctttgcc gctttttcca gactgcttca
      901 tggtttggtc gaaaaggtta ttgagcggga ccatcctgaa tctttagaca aatttcgtgc
      961 tgtcagaaaa cgcttcacag aaggttgggt tacgttacgg gaagctcttt ttcaaggtga
     1021 actgccgccc gacctcactg agcgggaata tgaggtggcc aggctggcgg ctcagggatt
     1081 gcgcaacagt gagattgcgg aaaaactggt gataactgaa agtacggtac gcacacatct
     1141 gcgaacaata ttccaaaagc ttcagatcga ccgtcgtgcc aaacttgctg aaaagctgaa
     1201 ataggattca gttgttagtg tttaaaagat cgtctattcg gacgatgtgc aaaccaaaat
     1261 cccattctat aatagtatat aggatggttt tttttcttta tcatgaaggg aatcatgaaa
     1321 ggagtatatg tatgaactac attaaaactg tactgcctat taaggactac cggctgttat
     1381 tggaaatgga ggggggaagc atagttatag tagacctttc cgcaaaattg aatacaatga
     1441 aatactccga gcttgccaac gataagttct tcaaaaccgc cgtaacagat ggtgattatg
     1501 tcatatgggg cggtgggcgg ctgcgtctga ctgtcaacga acttatggag gtagttttat
     1561 tgggataatt aagaaaacgt taaaataata gtctgctctt aagacacgta cgctttggcg
     1621 ttgtgtataa aaggcttatt gagataggag gtttaaatta tgtataattc tgttttaaag
     1681 cgtatggcat cggtattaat cattattgta atgctaatac ctcagattac cgctgctgct
     1741 gtagatggga caagttcaaa ggttcggtac agtaatgctg cacaatatgc ttcaacagga
     1801 aaaacaaact gttatcctga tatggggagg tggttaaaca gtgaggtgtt tgaaattgta
     1861 ccatcagata agggtgatga aataaagcct aaagacataa attcagattt tgaatattcg
     1921 tggctgatgc cccttcctga tgtgaaagat cagataaatg acgggctcag gtatgatata
     1981 gcactggata tattcggtca catatttgat tttgacatgg cagcagttta cgtagatttc
     2041 atggtgccta taggtgaagg tggaggagcc aggacattta ttactgatca atatctttta
     2101 gaagatattg atttatggaa gcatagaagc tttatggggt atataccgga agacgcaaca
     2161 tacatgaggt taaggcttgc ttcaaaaaaa gagaccggat ttctcgcaga caaagatgca
     2221 gctatcttct acagaaatat tgaagtctac ataactgatg acatcctacc cgtaccaatc
     2281 ggagtagagt atggatccag gtatgaactg ggtatggctg ctgcaggcaa atacaaggat
     2341 tattatggta taggcaataa tgtatatatt gatatccttt ttaatgagcc tatattcacc
     2401 aatgatccga actatcttgc atatataaga aatatgaacc atagtgacag agactcattt
     2461 gcaaaaaatc aactggctaa gaaatatcct aaagatagcc tggtattgca gcatgtttta
     2521 aataatgacg gtaaaacaac tgaacgtcaa tttggcgaat taaagcttaa gttcaaatac
     2581 aaaaattcag atggtgccga taaaacagga tatgctgtgg cagttgacag atctctttat
     2641 gatattgatg aaatatatcg ttatgattgc agtaaaaaaa taaagttcca atatacagta
     2701 caggatggtg atgaattcca ggcagatgat atctatgatt tggaactggt gggaggtata
     2761 attaccgaca attcctttaa tatgatgcct gacggaagaa gaaaaataaa ctttgataca
     2821 agttctgaca ataacataaa cgcagtctat agaagttatg cacagaactt cagagttgaa
     2881 accactcctc ccgtgctttt ggacattaac ggaaaaactc ccgatgggga aataaatccc
     2941 ggcagtgaat tggagatata ttttaagttt tcagaacctg tatatattgt gcttcctgac
     3001 ggagtcggag ctcatcgggc ttctgtcaac ctcaattcac aatatataga tgaatatttt
     3061 gaagtatcta atgcttatgg aagccccaga gcatattact tgggtggaag cggtactaac
     3121 atgctcaagt tcagatataa tgtgaaacat aataaatttg atcctttaga aatagttagt
     3181 acaggtttta tgggcacaga tacagatgat gacaataggg gaaatccata tggagaggtt
     3241 tatgtgagtg attctgccgg aaacatggca cctcttctgc agaataattc actgaagctt
     3301 tcggacaaca agctgtatgt agctgacaag atcccaccgg aattttctgc acaggttata
     3361 aaggcagaac ccggggagga tggcttttat ttagaaattg atgcatcaga taccggttcc
     3421 gggcttgatt acgatagcct gatgtttggt tttggaggat tggatgttag tatcagcaat
     3481 ttgcaaaata atatgaaacc tttgataccg ggaaaaaagt atcattcaat ggaattggcc
     3541 gagctgttcg ggatagatag gatagatatc agtaaattgg attccttcag agtgatagcg
     3601 tcgggaaagg ataagaaaaa taattattat ttcagcccaa gttccggtta tatatgggaa
     3661 attgttttaa gtattgatac tttaccccca atactctcca ggtactctat aaacaaggat
     3721 gcgtattctg ataaaaaagg ttcctttata ctttggataa aaaaaggaga cggaacatca
     3781 gtgcctgcag gggcagactc tgttttggct gttgaatata aatgggtaac agccggaact
     3841 aacccggata tggaagaatg gattcctgtt aacagtgtat atcatcaagg aatgggaaat
     3901 tatatcgcgg aaggtccggg accttcttac aatatatatg gtgacgcgga tctttatgca
     3961 aaagcagttg ataagcaagg gaatgaagca gttttttata tacccaaggc ccttacatat
     4021 gaagagataa ctaaagcact atttattaat caattatgtg atatggatat aagtttaggc
     4081 aggactgaag gacgactcta tgtagcttct gtaagaataa ggaataaaat tggaacagtt
     4141 attcctttta aaggattatg gtattgcatg actaaggaag caaatatgcc tgaactttca
     4201 gataaaacag ggatatggaa atataaagag ggcgacgagg ttagaactta tgactatttt
     4261 ggagataaat gggataagat taatggtttc tggtatatgc atatctgtgc tatggataat
     4321 aatgataagc ccatatgctc tgcaacagtt cccgaagcat tattgtttga ctttgaagca
     4381 ccgaacataa gtgtagatga agaaaaacaa gcattggacg acaagctgca aaaaccatat
     4441 gtgtatgcag tttcagacag aaaatataat gaaaatatct atacaaatga agattttgca
     4501 gaacttgaag tatatacatc agggaatgag ttttcttatt cattagatgg ggaaaattgg
     4561 agcacatggc tgccccttgc atcgaaaggc acttttaccc aaatggatag tgacaggtat
     4621 tatgaccttc ataacaacaa tggttacaat gtatataagg catatgtacc cctggagcag
     4681 aaagagggga atatgacttt ctatacccgc tatagaagag agggaatcca atcggaacct
     4741 gttgctgcgt cagtgatccg ggatgttact cctccaacag cgttggttaa ctacaaccct
     4801 tcatatacta cagactccag tgttatagca tccctttcaa gcatctcaga caatctatgc
     4861 cccggggaag tagtgaaagt tttaggagaa agaagccata cattctatga aaatggtact
     4921 tatagcttta ttattgaaga tgcagcggga aacaaagcga aggtgaaggc ggatgtaagt
     4981 aatatataca ttgaaccacc cgatacaagc aagccggtca taagtattac cggaacaccg
     5041 ggtggtgata taaccaataa agcagaatgc acaataaaag ttacagataa ttctccggtt
     5101 tcgataaaat atgtatggac atttgaaagg aactggaagg atgtacaaag cagtgaatgg
     5161 caaacaataa gtaatggcag tacagtatcc aaaacggatg aattagctgc caatgtcgct
     5221 ttaacttggt atcttcatgt actggcatcc gattcatcag gaaatacaga agtggcttca
     5281 aggtcatata ttatagattc tgttgctcct gttataagta tcaatcctaa tggcagcgaa
     5341 aatgtggttg aaagtgtttc gaccttaatt acaattgatg atgcattgaa cacagaagga
     5401 acatatatat ggcattcgag tgatgaattg ctacccgatg acgatgacgg ctggaaagac
     5461 gtaccggata atggtgttgt aagtttgtca ggtgttgacg gaatatggta cctgtatgtc
     5521 aaggctgtag atgctgcagg gaatgtcgga acctttaaaa caaatccttt taatatggtt
     5581 tcggaatata tagaaccata tgtggtttat tcaggtgaag agaaagggaa aattagagct
     5641 tttcttgtat cacaatgccc tataactgta gatggaccgg tatattgcga ttttgaaagg
     5701 gatttggagc gtgagaatcc tagagaggca tatactgttt cttatgactt tagttactcc
     5761 tatggtggtg aagaaaaaca cacattgact gcacagtggg attttccgga gatcccttcg
     5821 tatatagag
//
LOCUS       DAOSDD010000259         5825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4904_length_5825_cov_5.985442, whole genome shotgun sequence.
ACCESSION   DAOSDD010000259 DAOSDD010000000
VERSION     DAOSDD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4904_length_5825_cov_5.985442"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      370..475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            581..3103
                     /locus_tag="PK733_16875"
     CDS             581..3103
                     /locus_tag="PK733_16875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOJ12244.1"
                     /translation="MTDNKQIMEGLNSAEAKRLQQQYGKNELTPQKKEGFLQKVLHII
                     FEPMFLLLIVAAIIYFILGEPRDGAIMLIFVIGIISIDVIQEWKTDKTLSALKDLSAP
                     HIKVIRDGKEMMIASTDLVPGDLMMIYEGVKIPADGIVVKCSDLCVEESSLTGEAEGV
                     WKVTSENADLSNDYWRKDYCYAGTLVTQGTGTVLVDKIGANTEYGKIGMNVAAAPDEP
                     TPLQKQTGKLVKTCAGIAGVLFVLVGVVTYFNIPDHALGDRLIESILAGITLAMAMIP
                     EEFPVILTVFLSMGAWRLAKKQSLVRKLPSVETLGSVSVLCVDKTGTITMNRMVVQET
                     WAPNGDEHTLIETMGLGCEIDAYDPMEKAMLAYCDSHGISKEHLFNGELISEYAFTNE
                     LKMMGHIWRRNGRISIAAKGSPEQILTICNMTGKDRETAENKIIEMSKEGLRVIAVAN
                     ATLASEDEIPSSITECSLNLCGLIGLADPPRESVKSDIAVCRKAGIRVVMITGDNGIT
                     ASSIARKIGMEHSDNIITGDMLNEMSDNELRKAVKDVSIFSRVIPEHKMRIVKAFKEN
                     GEIVAMTGDGVNDAPALKHADIGIAMGKRGSEVSREAADLILMDDNFTTIVETVKDGR
                     RIYDNIRKAVGYVFTIHIPIALTSLLAPILGIAPAALMLLPLHVVLLELLIDPTCSIV
                     LERQPAETDIMERGPRDPKEKLLNIRTILKSVIQGLVVFVASFGTYYAILNGDPTNAS
                     IARAMGLTIIMLSNLFLVQVNSSDRDFAFQSIARLSKDWVMWAVSIGTLLMLAIILYT
                     PLSGFLKLSPLTTVQFFTSVGIAVAAVFWYEIIKLIKRIRTR"
     gene            3337..4068
                     /locus_tag="PK733_16880"
     CDS             3337..4068
                     /locus_tag="PK733_16880"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ12245.1"
                     /translation="MKNDYTPIYKKIKDEILNDIATGVYEPGDMIPNQNYFTEKYGVS
                     RTTVREAINELKLRGILDTYKGKGTFVMTNEGGFYGSNRLEGLSQNRIKEGDPVCSKI
                     LSIKVIPAEKRIAKALGIQSSHKVLSLRRLRLIGEVCIAVEHSYLNYDYVKNIDFENA
                     NLETGSLYKLLQTQAGIVFKYADEEIHSVLANPEIAEYFNIECMSPILFVKRVTTTQS
                     DIPLEYCENYERGDIYGIRIRTINL"
     gene            4344..5357
                     /locus_tag="PK733_16885"
     CDS             4344..5357
                     /locus_tag="PK733_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ12246.1"
                     /translation="MTKYEKILGSLLGAAIGDTMGAATEMRTTKQIEEFFGGYVTEIL
                     TPPNDTFARGAEAGFVTDDFSLAYFTAQTICDNNGEINEKVAIQSLLLWAEHEEYFGK
                     YVGPTTKAAIKRLLNETVEETYPFLKYDCAKATNGSAMKIGPVGLFRPGDIDRAIEDA
                     VTICLPTHNNNLSISGACAVAAAVSKAMENNANVYDLVQAGLYGARKGFEIGGKKGIV
                     LAGQSVERKINLAVEIALRSDSLKTAMTDIADLIGSGLMIHEAVPAAFGLLVAAKGNA
                     MESICAGVNIGYDTDTTATIIGAMTGALQGVSAFSKEHLELINFKNNFDLEELACRIE
                     ELL"
     gene            5357..>5825
                     /locus_tag="PK733_16890"
     CDS             5357..>5825
                     /locus_tag="PK733_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ12247.1"
                     /translation="MNDQDRRYIIALGKVCVDEYYEANSWPNIGEKKLVKPMKKMIGG
                     MIANAACVLSGYKVKTYLLDLLNDSTTTTEIIEDLNKYNVDTSLLLYDSSIADSKCII
                     FLVGGERVIFVVDDKRPGIRLNKEQLKMLAGAEYIYTTIPDLKELEQPFELISQ"
BASE COUNT         1832 a         1016 c         1346 g         1631 t
ORIGIN      
        1 accctttctg acttctcgga cacgggagaa atagcttcct gggcaagaga ggcaatgacc
       61 ttgctggtag aaaccggaac catcaatggg aatgccggta agcttacacc acttaataca
      121 accacaaggg cagaaatggc acaggtttta tgtaatctga tgacagagta agggccgcca
      181 aggccctacc aggttcaagc agcatctttt ttgtatcctc aaggcatgtg gaatccatat
      241 gataaaatac attttaattg tgtgcctagc tttttaaaaa gtttgttgac agagacagct
      301 acagagcgta taatacaaag taatctcttt gcactgcatt acattctttt taaaaggaat
      361 gcgagatcaa cagtacaaac tgagcaggcg atgggccttt tattgaggta catggggccg
      421 ggctacctga cgagtggcag cggattgcgt tcacatgcac atctgtggga cagtagtatg
      481 ttccataggt gtgctttttt atacctgacg gaacgacctt gaatggagtg ccataagaga
      541 aatcaaacgc cgccgaaagg cgaaaacgga gggttctttt atgacagaca acaagcaaat
      601 tatggagggt ttaaattccg ccgaagcaaa gcgattgcag cagcaatacg gcaaaaacga
      661 attaacacct cagaaaaagg aaggttttct tcaaaaggtt cttcacatta tttttgagcc
      721 gatgttcctt ttgctgattg tagctgcaat catttatttt atcctcggtg aaccgagaga
      781 tggtgcaata atgctgattt tcgttatcgg tattataagc atcgacgtta ttcaggaatg
      841 gaaaacagac aaaacattga gtgctcttaa ggatttatcg gcgcctcata ttaaggttat
      901 aagagatggt aaagaaatga tgattgccag cacagatctt gtgcctggtg acttgatgat
      961 gatatatgaa ggcgtaaaga tccctgccga tggtattgtg gtaaagtgca gtgatctttg
     1021 tgtggaagaa tcttcgctta ccggtgaggc cgaaggtgtc tggaaggtca ccagcgagaa
     1081 tgctgatctg tccaatgatt attggcgcaa ggattattgc tatgcaggta cacttgttac
     1141 tcagggtact gggactgtat tggttgataa aattggtgcg aataccgagt atggtaaaat
     1201 cggcatgaat gtagcagctg ctcctgatga gccgacacct ttacagaagc agacaggtaa
     1261 attggttaaa acgtgtgccg ggattgcggg tgtgttattt gtgctggtgg gcgtggtcac
     1321 ctatttcaat atacctgatc atgctttggg tgaccgtttg attgaaagca tcctggctgg
     1381 cattacgcta gccatggcga tgatccccga agaatttccc gttattctga cggtgttcct
     1441 ttctatgggc gcatggcgac ttgcaaagaa acaatcgctt gtacgcaagc tgccgtctgt
     1501 cgaaactctt ggctctgtat cagtactgtg cgtggataaa acaggtacca ttacgatgaa
     1561 ccggatggtg gttcaggaga catgggcacc gaacggtgat gaacataccc ttatcgaaac
     1621 tatgggactt ggctgcgaaa tagatgccta tgaccctatg gaaaaggcaa tgcttgctta
     1681 ctgtgacagt catggaatat cgaaggaaca tctcttcaat ggcgagctaa tcagtgagta
     1741 tgcctttacc aatgaactga aaatgatggg gcatatttgg cgcagaaacg gtaggatatc
     1801 aattgccgcc aaaggttctc cagaacagat attgaccata tgcaatatga caggaaaaga
     1861 cagagaaact gccgagaata agattatcga aatgtccaaa gaagggctgc gtgtgatagc
     1921 agtggcgaac gcaacactag cgtcagagga tgagatcccc tcaagcataa ctgaatgcag
     1981 cctgaatctc tgtggtctga tcggtcttgc cgaccctcca cgtgaaagtg ttaaatccga
     2041 tattgcggtg tgccgcaagg ccggcattcg tgtcgtgatg atcacaggtg acaatggaat
     2101 cactgcctct tccattgcca ggaaaatagg aatggaacat agcgataaca taattacagg
     2161 cgatatgctc aatgaaatgt ccgacaatga attacggaaa gcggtcaagg acgtgtctat
     2221 tttctcccga gttattccag agcataaaat gaggattgtc aaagcattta aggaaaatgg
     2281 cgaaatcgtt gccatgacag gtgatggcgt gaatgacgca ccggctctga agcatgccga
     2341 catcggcatc gctatgggca agcgtggaag cgaggtttcc agagaagccg ctgatcttat
     2401 tttgatggat gataacttta cgaccattgt tgaaacagtg aaagacggca gaaggattta
     2461 tgacaacata cgaaaagcgg taggatatgt atttactatt catattccga ttgcacttac
     2521 atcgctgctg gctcctattc tcggaattgc ccctgctgca ttaatgcttt tgcccctgca
     2581 tgttgttttg cttgagcttc tcatcgaccc gacctgctcc attgtattgg aacgccagcc
     2641 ggctgaaaca gacattatgg agcgtgggcc gcgtgatccg aaagaaaagc ttttaaatat
     2701 aaggactata ctaaaaagtg ttattcaggg acttgtggtt tttgtcgcat ctttcgggac
     2761 ttattatgcc attcttaacg gtgatcccac taatgcatct attgcccgtg caatggggct
     2821 gactatcatc atgctctcca acctgttcct tgttcaggta aacagctccg accgtgattt
     2881 tgcgtttcaa tcgattgccc gcctttcaaa agactgggtt atgtgggcgg tcagcatcgg
     2941 aacgttactt atgcttgcaa tcattcttta cacgccgctc tcaggttttt tgaaactttc
     3001 accgctcaca actgtgcagt ttttcacatc ggtaggtata gcagtggcgg cagtgttttg
     3061 gtatgaaatc ataaagctga ttaaacggat ccgtacgagg tagacctatg gaaatgatat
     3121 atggcttgtt aacataaggt agcataataa aaaaaagcga atgcttgcgt atacgagatt
     3181 gaggagttat tcattaatgg caaatgactc atatggctga aaaactaaat tgagatttaa
     3241 atacattccc tatataataa aatcataaat aaatggcaag ccatatagtc atatagtcat
     3301 ataacatata attttaaacc atagagggag tgtattatga aaaacgacta tacacctatc
     3361 tacaaaaaga taaaagatga aattttgaat gatatcgcta ctggtgtata tgaacccgga
     3421 gatatgatac ctaatcagaa ttatttcact gaaaaatatg gtgtaagccg tactactgtt
     3481 cgagaagcaa ttaatgagct taaactcagg ggaatattgg acacatataa gggaaagggc
     3541 acatttgtca tgacaaatga aggaggcttc tatggttcta atcgactgga aggtctcagt
     3601 caaaacagaa tcaaagaagg agatccggta tgttctaaga tactttccat taaagtaatt
     3661 cctgcagaaa agcggattgc caaagcgctg ggaattcaaa gcagtcataa agtattaagt
     3721 cttaggagac ttcgtttgat aggagaagta tgtattgcag tagaacattc atatcttaat
     3781 tatgattatg taaaaaacat agattttgaa aatgctaatc tagaaacagg atctttatat
     3841 aaactattgc aaactcaggc agggattgta ttcaaatatg cagatgaaga aattcattca
     3901 gtattagcta atcctgagat tgcagaatac tttaatattg agtgtatgag tcctattttg
     3961 tttgttaaaa gggtcaccac tacacaatca gatatacctc ttgaatattg tgagaattac
     4021 gaacgagggg atatatatgg aatacgtatc cgaactatca atttataata ttaatattat
     4081 tacctgcctt atcttaaaca tagtgttgca aaagtgaatc ggataaattt agtacatagg
     4141 taagtgtttc aaaaggttgt ataaacacta tggttgctat aacaactcat gtaaataaat
     4201 gacataagag aaataaaata acaataatgg attagattaa ctatggatat atagctttct
     4261 taagtacaat atctttgtat aagaaatata tagggctata aactatatgg ctatttaaaa
     4321 ggaagccaaa agaggaggaa aatatgacaa aatatgaaaa aatactagga agccttttag
     4381 gagccgcaat tggtgatact atgggcgctg ctacagagat gagaactact aagcaaattg
     4441 aggagttttt tggcggatat gttaccgaaa ttcttacacc gccaaacgat acctttgcta
     4501 gaggtgcaga ggcgggattt gtgacagatg attttagtct tgcctatttt acagctcaaa
     4561 caatttgtga taataacgga gagatcaatg agaaagttgc catccaatca ttgcttttat
     4621 gggctgagca cgaggagtat tttggtaagt atgttggtcc taccactaaa gctgcaatta
     4681 aaaggctgct taatgagaca gtagaagaaa cttatccttt tctaaaatat gactgcgcta
     4741 aggctactaa tggatcagct atgaaaattg gacctgtggg actatttagg ccaggagata
     4801 ttgatagagc tattgaagat gcagttacta tatgtttgcc tacacataac aacaaccttt
     4861 caatttccgg agcatgtgct gttgcagcag ccgtaagcaa agcaatggag aataatgcta
     4921 atgtctatga tcttgtacaa gctggtttat atggggcaag gaaaggtttt gaaataggcg
     4981 gtaaaaaagg tattgtctta gccggtcaat cagtagaaag gaagattaat cttgctgtag
     5041 aaatcgctct acgttctgat tcattaaaaa cagctatgac ggatattgca gacttaattg
     5101 gcagtggatt aatgatccat gaagcagttc ctgctgcttt tggattattg gtggcagcaa
     5161 agggtaatgc aatggaatca atatgtgcag gagtcaatat tgggtatgac actgatacta
     5221 cagctacaat aattggtgca atgacaggtg cattgcaggg tgtaagtgca ttttcaaaag
     5281 agcatttaga attaataaac tttaagaata attttgactt ggaagagctt gcatgcagaa
     5341 tagaggagtt attataatga atgatcaaga tcgccgctat attattgctc tgggaaaggt
     5401 ttgtgtagat gagtattatg aagctaacag ctggccgaat attggtgaga aaaaattagt
     5461 aaagcccatg aaaaaaatga tagggggcat gatagcaaat gctgcttgtg ttctgtcagg
     5521 gtataaagtt aaaacttatc tattagactt acttaacgac agtacaacca ctacagaaat
     5581 tattgaagat ctaaataagt ataatgttga tacttcgctt ttgctttatg actcgtcaat
     5641 tgctgacagc aaatgcatta tatttttggt aggcggtgag cgagttatat ttgtagtaga
     5701 tgataagcgt cctgggatca gacttaacaa agaacagcta aaaatgctgg caggtgcaga
     5761 gtatatttac actaccattc cggacctcaa ggagctggaa cagccttttg aactaattag
     5821 tcaat
//
LOCUS       DAOSDD010000260         5802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_4934_length_5802_cov_5.551592, whole genome shotgun sequence.
ACCESSION   DAOSDD010000260 DAOSDD010000000
VERSION     DAOSDD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4934_length_5802_cov_5.551592"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="PK733_16895"
     CDS             complement(<1..1326)
                     /locus_tag="PK733_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOJ12248.1"
                     /translation="MILFDNVEYDRFFNPLCCKNRRIYYECIMHLIEKSKAVPLLYET
                     DAKDCIILYLRNCTYAVEDESGSDNGDELISSKKSDAENAGTILRYFRSCSWISEREI
                     GRSGDNIASVMPYCRKMIDSIQRIFDRDTSGLLTNHIFAIYDTLKSALDAEHGRTIRP
                     YSNILVPLTNNVSDLKNELQILKDSIRTIMRIVIKMTEVNTFGQFLIKDEMMNLFFND
                     YFFIKKDGLIPGYIAEIEKMLRQIINTDVYNNMIREYETLKQTDEYKAREIIDGQLTE
                     IQGFINYDYVKEMDYIDKKINNYYNLYSTRMLMVLSNNTNMQTYLNDLLMILKEMDSE
                     EREIVLAEISKSYRLSSYRYIGRKSVERKKKRHPNIKSGAIVISSLSDDEKAKLTQEL
                     LHEYPDRYSVAQVARYFHTLLSDKVSVETSEQNIKNRDEAMMIAASIIYS"
     gene            1785..2069
                     /locus_tag="PK733_16900"
     CDS             1785..2069
                     /locus_tag="PK733_16900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04540 family protein"
                     /protein_id="HOJ12249.1"
                     /translation="MSQTCLNGWFYRPSLYFVPGSVVLAAQIIKACDDYIALKITEKH
                     LKELIFHYASYHGNKLFAAKGVNPTIVNRIGKKRLELLNIMLEGFQYRLL"
     gene            complement(2561..2830)
                     /locus_tag="PK733_16905"
     CDS             complement(2561..2830)
                     /locus_tag="PK733_16905"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ12250.1"
                     /translation="MRKASNGREYANKLSVFLNFLSGRNIEYDQASRNDVLAFIDALV
                     YGLSKNDNVFNVKNASVTYNTLSLYITVITEFYKWLHQKVNGKCR"
     gene            complement(2846..2974)
                     /locus_tag="PK733_16910"
     CDS             complement(2846..2974)
                     /locus_tag="PK733_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12251.1"
                     /translation="MMDACGDRHVYRVVPAKLPVCGKITKRWMIVDNKILVYELNE"
     gene            complement(3415..>5802)
                     /locus_tag="PK733_16915"
     CDS             complement(3415..>5802)
                     /locus_tag="PK733_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12252.1"
                     /translation="YYEIGIQPIYDYLYAIIIIDKIKRNAFEKLPDNLKSNNGTLQMA
                     SIILLNDYNTLLSDYSVFERSLKNNDLYDLIFYALANVSPLIAKGYVPLIKSLLTQGA
                     FAVKAITNKVFLSVARIEGHPLSPIILHEFLMEFENAGQRDLFWSVPSILVFSMSKWN
                     NRIELNLQNETYKLTSGDKAIGLPLIYAWSLTSVDNVKRAYYRKELFNWSILQPLEFI
                     ELFKITYQANDPQMKEELLSIAMGIVFTMPTDSLVIDTFGKWVLEEVFTDGKIQNTFD
                     AAIRYYSRAIAERAYSCGFISQKQILECRPPYKSSSKCISMNIDATEGTVMSGYGPID
                     YDLSRYVLCDSIKSQFFNLPFGYDRNDNNYVYYKEADEFLSVHAKQLNIKNLEHHQFV
                     LSASYSYLLNCGWNEEDFYGEPNGGKKGEVLGADIAIIRQYHPATHGSKSNVMSFAEK
                     YVWCAKNEILGYLADRLPFCDAETQHMVEDYGVLEDFIIPAQEIDQQNPEELSDNEKW
                     YLPEKLSPTIDYLQNRTEKDIIQWINEVRIPNFELWININKQGYNVSLDYDIDLLTLF
                     SYNCQSEHNTGVETMMWISSGFIHDAQFKPFLNDLNINNNDIVHIFREPERFTASTES
                     SCYLTPKEICWMTWKKDKNSKVEIYDLNEPDKIKYKIIKAVEDTVCNFDEYGEIYYKL
                     PSKYVRSILEIIDGNGYRYYDQNKKLVGISFEYGEKWHDYQKNLCVDKNTLEQTLSAE
                     NMKMFWVIRLLREPSSKSKEKFSKLYYRRDRCWIVWFENGKMNCELFFDGADRLI"
BASE COUNT         1826 a         1160 c          769 g         2047 t
ORIGIN      
        1 agaatatata atacttgctg ctatcatcat agcttcatct ctatttttta tattttgttc
       61 actggtttca actgatactt tgtcacttaa taatgtgtgg aaataccttg cgacctgagc
      121 cacactatat ctatcaggat actcatgtaa aagctcctgt gttaattttg ctttctcatc
      181 atctgataaa gagcttataa caattgcacc gctttttata tttgggtgtc gttttttctt
      241 tcgctcaacg gattttcttc cgatatatct ataggatgac agccggtaac tctttgaaat
      301 ttcagcaagt actatttctc gttcttcgga atccatttcc ttcaatatca tcaacagatc
      361 attcaaataa gtttgcatat tcgtattatt actgagtacc atcaacatcc tggtcgaata
      421 cagattataa taattgttaa tctttttatc tatgtaatcc atttccttaa cgtagtcata
      481 gttgataaat ccctggattt cagtaagttg tccatcaata atttctctag ccttatactc
      541 atccgtttgc tttaatgttt catattcacg aatcatatta ttatatacat cagtatttat
      601 aatctgtcta agcatctttt caatttctgc tatatagcca ggaataagtc catctttttt
      661 tataaaaaaa taatcattaa agaataaatt catcatctca tctttaatca aaaactgacc
      721 aaaagtattc acttccgtca ttttaatgac tatacgcatt attgtgcgta tactatcttt
      781 caaaatctgg agctcatttt tcaaatccga aacattattc gtaagcggaa ccaaaatatt
      841 tgaataaggc ctgattgtcc gtccatgctc tgcatccaat gcagatttta acgtatcata
      901 aatcgcaaat atatgatttg tcagtaaccc actggtatca cgatcaaaaa tacgttgaat
      961 cgaatcaatc atcttcctgc aatagggcat tacagaagca atattgtccc cgcttcgacc
     1021 aatttctcgc tcagaaatcc agctacaaga acgaaagtat cttaatatag taccggcatt
     1081 ctcagcatca gactttttgc tgctaataag ctcatcacca ttgtcactac cgctctcatc
     1141 ctctactgca tatgtgcaat tgcgcaaata gaggatgatg caatcctttg cgtctgtttc
     1201 ataaagcaat ggaacagcct tcgatttttc aatcaaatgc ataatacact cataatatat
     1261 ccttctgttt ttacaacata gcggattaaa gaaacgatca tattccacat tatcaaataa
     1321 aatcatatgc aatacctctt ttgcttaaca aatactgcta tttttcctgc ttcatcattt
     1381 actttacaaa tagctgcaac agactactaa cagcttgatt cacgttatta taattgatgt
     1441 aatatcacct taattatata gcatcttcca gtatctctca actgaaagcg ttataattca
     1501 gcaaatataa tgattgtttt gattatttat tgattataag atccagtaga tatgttcgca
     1561 caaacataaa aaccattgag catcatctac gcactcaatg gcttaaacta ttgacattac
     1621 tatattttat aacttttcaa gcttcacaac tacctcgcaa tggctcattc aaggaaacat
     1681 gtcaaattta atacttctga aaaagtgcac ccacattcat aaatatgcaa tgccttcatc
     1741 cattgtatcc gttgttttgg gcttagacca gtaacacact gcagttgtct caaacttgct
     1801 taaatggctg gttttatcgt ccttcgttgt attttgtacc aggctccgta gtacttgccg
     1861 ctcagataat taaagcctgt gatgactata tagccttgaa aattactgag aaacacttaa
     1921 aggaacttat ttttcactat gcttcatacc atggtaataa attatttgct gcaaaaggtg
     1981 ttaatccgac catcgtcaac cgtataggga aaaaaagatt agagcttcta aacattatgc
     2041 ttgaaggttt tcaatataga cttctttaac ctttgatata tctgccggat tttaagttgt
     2101 gtttttaaat tttaacctga tgcccggctc tgtgtcgata aagcgctctg caggaatgca
     2161 acacctaact ccacaaccgg gctaaaggat atatcctttc aggatgtagt atgaaaattc
     2221 tacattaatt tctatagacc ggtaaagtaa gcacaagttt attttccgct cttgtatttt
     2281 tttcatttct tgtccctgaa gtttatcttg tctaaactac tgcaaattag cttgtctcac
     2341 aacagcagtc cttccaatgt aaccataaat atcgatttgt ctctgagtgt aagaaaatta
     2401 gaaatgagga tttccttttc ctcctccgta taccacttga tatattccct gcttgatttc
     2461 agcgttctga ttcttttttc tataatggta aagtagttga aatcatatat ccgcccgtag
     2521 aaatataatt tccctgataa ttttcatctt gtaccgtcta ttatcggcac ttaccattca
     2581 ctttctgatg aagccatttg tagaattctg ttatgaccgt tatatacaag ctgagtgtat
     2641 tgtaggtaac agatgcattt ttaacattaa aaacattatc atttttagac aatccataga
     2701 ctagagcatc aataaatgca agcacatcat tccttgatgc ctgatcatat tcaatatttc
     2761 ttcctgataa aaaattcaaa aatacactga gcttatttgc atattctctt ccattacttg
     2821 cttttctcag gcttttgagt tcaacctatt cattcaattc atatacaagg attttgttgt
     2881 cgacgatcat ccatctcttt gtaatcttac cgcatacagg tagttttgct ggaaccaccc
     2941 tataaacatg cctatcgcca caagcatcca tcatctttac actccagata atattatttt
     3001 gtttgttaaa cactagacat cattgatatg tatagtattg ccaatattta tattattatc
     3061 cgtcatagtg aacatttttt acagtgcctg gtctctaaag aaaaccacgc ctccttttta
     3121 ccgcctcctt tttaatttat gaatacctaa tatatttatt aaagtagatc ccaaataccg
     3181 atttcaacca ccagccggaa ccgattgaaa agtttgtgcc gtggtcgaaa gagcttctgg
     3241 caaactgcta taaaatataa taaaaagtag tgattcggtt ggtacgtttc caaccttcaa
     3301 ttttccccgg gtgcttgaat ttggcaatta cttttatatg taaaaagaga acataagcgc
     3361 aaagcctata tcttcattat tcactctatg atgtagttat aaacaataaa caagctaaat
     3421 tagtctgtct gcaccatcaa aaaataattc acaattcatc ttgccattct caaaccacac
     3481 aatccaacac cgatctctcc tataatacaa ctttgagaat ttttcttttg acttgcttga
     3541 tggttctctt aacaatcgta taacccaaaa cattttcata ttttcggcac tgagcgtttg
     3601 ctctaaggta tttttatcaa cacaaagatt tttctgataa tcatgccatt tctcaccata
     3661 ttcaaagctt atacccacta attttttatt ttggtcataa tatctgtaac cattaccgtc
     3721 aattatttcc agtatacttc ttacatattt tgaaggtaat ttataatata tctccccata
     3781 ttcgtcaaaa ttgcatactg tgtcttccac agctttaata attttatatt ttatcttatc
     3841 tggctcattc aaatcatata tttcaacctt tgagtttttg tcctttttcc aagtcatcca
     3901 gcatatttct ttaggggtca aatagcaact agattctgtt gatgcggtaa aacgttccgg
     3961 ttccctaaat atatgtacaa tatcattatt atttatattt aaatcattta gaaatggctt
     4021 aaactgcgca tcatgtatga atccagatga aatccacatc atagtttcaa ccccagtgtt
     4081 atgttctgac tgacaattgt aagaaaataa cgtgagaaga tcaatatcat aatctaatga
     4141 aacattatag ccctgcttat ttatattaat ccataattca aaattaggga tacgcacttc
     4201 gttaatccat tgaataatat ctttttctgt cctgttttgt aaataatcaa tagtcggcga
     4261 taatttctca ggtaaatacc atttttcatt atctgacaat tcttcaggat tttgctggtc
     4321 aatctcttgt gctggaatta taaaatcttc taaaacacca tagtcctcaa ccatgtgctg
     4381 agtttcagca tcgcaaaaag gtagtcgatc tgcaagatat ccaaggattt cattttttgc
     4441 acaccaaaca tatttttcag caaaagacat aacattactt tttgaaccat gagttgcagg
     4501 atgatattga cgtataatcg ctatatctgc tcctaaaact tctccttttt ttcctccatt
     4561 tggttcgcca taaaagtctt cttcattcca cccacaattc aataaatatg aataactagc
     4621 agacagcaca aactgatgat gttccaaatt ttttatgttt aattgttttg catgcaccga
     4681 caaaaattca tccgcttctt tgtagtacac ataattatta tcgtttctgt catatccaaa
     4741 tggtaagtta aaaaattgac ttttgattga atcacaaaga acatatctag acaaatcata
     4801 atcaataggc ccatatccag acattactgt tccctcagta gcatcaatat tcattgatat
     4861 acatttacta ctacttttat aaggtggcct acattccaat atctgttttt gagaaataaa
     4921 tccacatgaa tatgcccgtt cagcaatagc gcgagaatag tatctgatcg ctgcatcaaa
     4981 ggtattctgt attttcccat ctgtaaacac ttcttcaagt acccatttgc caaacgtatc
     5041 aattaccaaa ctatctgtag gcattgtaaa aacaattccc atggcgatag acaatagttc
     5101 ctctttcatt tgaggatcat tcgcctgata agtaattttg aatagttcta taaattctag
     5161 tggttgtaga atactccaat tgaataactc ttttctataa tatgcacgtt taacattatc
     5221 aacagaagta agcgaccaag catatatcaa tggcaatcca attgctttat caccactagt
     5281 taatttatag gtttcatttt gcaagtttaa ctctattcga ttattccatt tggacattga
     5341 aaacactaat atactcggaa ccgaccagaa aagatctctt tgtccagcat tttcaaattc
     5401 catcagaaat tcatgcaaaa ttataggaga caacggatgg ccctctattc ttgcaacgct
     5461 aagaaaaact ttatttgtaa ttgcttttac agcgaaagct ccttgtgtta aaagactttt
     5521 aatcaacgga acatatcctt ttgcaataag tggtgaaaca ttagctaacg cataaaaaat
     5581 caaatcatat aaatcattat tttttaagct tctttcaaaa acagaataat cactcaaaag
     5641 tgtgttgtaa tcatttaata gaataataga cgccatttgg agcgtcccat tgttactctt
     5701 caaattatcc ggcaactttt caaatgcatt tcttttaatt ttatctataa ttattattgc
     5761 atataaataa tcataaattg gctgtattcc aatctcataa ta
//
LOCUS       DAOSDD010000261         5707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5040_length_5707_cov_3.745046, whole genome shotgun sequence.
ACCESSION   DAOSDD010000261 DAOSDD010000000
VERSION     DAOSDD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5040_length_5707_cov_3.745046"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(400..2112)
                     /locus_tag="PK733_16920"
     CDS             complement(400..2112)
                     /locus_tag="PK733_16920"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOJ12253.1"
                     /translation="MKKLLKTISVVLIMTLVLTSGITVAFADSWKKPGKLTVNNRNNT
                     LKVEQVQEQLRQRKNEREKEIQKYMDKVKEKLQEKSKVSKEKELKELEGKIKQLEKEV
                     RELHNEIEKLKKDRGGILQSGNAKKDKAPAVIKCGRFLIPTRPIIKASGAELEWDEKN
                     KTLTIEKDKTTITINLDNGEIKVNGDKLDLSKYGWANNNGRCVPISLITRILRDKYEI
                     DKDEKDDHDEDEDEDEDEDEDEDYDGKIEKIISVNDNVTGTANGQFQYIGDWKYAAQT
                     GAFMNDNHWSNSTDDFFRVKFTGNQIKLYGAKDPSHGIAAVSIDDGDEVIVDYYAAER
                     SDNVLIYTSPILKDGEHTLKVRVTGAKNKSASDKHITVDKVEIVELVEPEGVLEGVIA
                     KAPDSVNLSNEGKVDWVHWGNTGVTGYNRKVTTKSKPDITNYTQIGTGTLSWVSNNPV
                     KFSWNGGTPTSQAANIASAIYMSNVSSGFQFAVPADTNEKTLRVYVAAWDATGKLEAV
                     LSDGSAAPYTVDIVSDSGIACKVVTIRYKAKSEGEKLTVRYTVKSFKGNNISNISLQA
                     ATLK"
     gene            complement(2344..3897)
                     /locus_tag="PK733_16925"
     CDS             complement(2344..3897)
                     /locus_tag="PK733_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12254.1"
                     /translation="MIVVMKGLVVEIAGKHAIILKTDGEFEKVKNNNFQVGYEINLNQ
                     YNGFSKETLGNTSIIRKIVSIAAVFAIIFGLTYGVYSYGMPYTYVSVDINPSVEITAN
                     IFDRIINIEGINDDGKKLISLKQYRNKKVRDGIGNIIKSAVEEGYIKPEYSNMLLLTI
                     SSKDIKKFGIIEKDIEEAVGKEINVSEIDTSIYIENVEVKKHDDAKNLGISSGKYLLL
                     EKMKESEPEIVYEDWKEAPVREIMKSVIEKKNEEIFENKMQRIQQKIGKEQEKELGKQ
                     EKEDALKVEEEKETDDKGKGNGKGNGKDNGKDNGKVKEKAEGKGQENAKGKNYEYKND
                     KGTDRGNDAKNNKGKGSSGNSSVNKSKDNLNDGNGNSKNKKESNGKNENIDETEDDKD
                     MDKGVKEDIDGEDEDMDEDIGEDINDINGIDDIDSINNIYSIDNEIFDEEIVYDEIDN
                     NTKKDKGKSNSDKGNSDKGNSKDNGNNKDKANSKEKGESIDKGESKDKGESKGNSQGN
                     QGKKNIRKK"
     gene            complement(4022..4738)
                     /gene="sigI"
                     /locus_tag="PK733_16930"
     CDS             complement(4022..4738)
                     /gene="sigI"
                     /locus_tag="PK733_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HOJ12255.1"
                     /translation="MKYASINERVEDIKGKDNDEEINRFVEEYKPFIASCTEKFTGKY
                     VTYGSSDELSIALIAFVESIKSFDSSKGNFLSFAQGVIRRRLIDFYRKEKKHMNVISL
                     NEYFIEESGEEVDLSTEEAIEKYNNEEISEYRRLEIEELKKELLKWDITFNDLVMASP
                     KHSDTRETCRNIVKFIISKPELIEVIKTKKYIPIAEIEKTLKVSRKKIERLRKYIIAI
                     VTVITGDYHYIREYVRDYIP"
     gene            complement(5131..>5707)
                     /gene="fucI"
                     /locus_tag="PK733_16935"
     CDS             complement(5131..>5707)
                     /gene="fucI"
                     /locus_tag="PK733_16935"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356557.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOJ12256.1"
                     /translation="GEQTKDGKPAMKPFWEITPEEAAKCLDATLWRPASVGYFRGGGF
                     SSDFLTKGGMPITMSRVNSIKGLGPVLQIAEGYSVDLPEKMHEILDNRTDPTWPTTWF
                     APILTGNGPFLDVYSVMANWGANHGAFSYGHIGAELITLASMLRIPVCMHNVSKDRIF
                     RPSTWSAFGTNDLEGADYRACMNFGPLYGKK"
BASE COUNT         1519 a         1240 c          690 g         2258 t
ORIGIN      
        1 gcggcgtaac gtcagctcct ggtaaatatt tcttcacttt taaaaaatgc accctttagt
       61 tcatgcagca ttggcaaagt cgtgtgagat ttcaaataaa tattcaaata aatagtagta
      121 tagtcattcc aggtacagta aaagtaaatt tgaaaaaaca caaaataatt ttcttccaat
      181 cagctaaagc taatcaaaaa agaaatcctg taaatttctt tacaggattt cttttttaca
      241 tttctattat tggtgggaag tattgctttg atcaacaatt ctgtttatat ctgataaaat
      301 atttatttgc actagtgaag gtgtatttat tttttgttac accacattgc agcatataaa
      361 ttcttaaggc accctttaac aactgtttgt aaattaagtt tacttgagtg tagcggcctg
      421 caggcttata ttactgatat tattaccttt aaaacttttt acagtatatc ttacagtaag
      481 tttttcacct tcagatttcg ccttgtatct tattgtaact actttacacg caatgcccga
      541 atctgaaact atatcaacag tatatggagc tgcacttcca tcacttagta ctgcttctaa
      601 tttccctgtg gcatcccatg ctgctacata tactcttaat gtcttttcgt ttgtatctgc
      661 aggtactgca aattggaaac cactgctcac attagacata taaatggctg atgcaatatt
      721 tgcagcttga gatgttggag ttccaccgtt ccatgaaaac ttaaccggat tatttgaaac
      781 ccacgaaaga gttcctgttc ctatctgggt ataattggtg atatcgggtt tggatttggt
      841 agttactttt cggttatacc ccgtaacccc ggtatttccc caatgcaccc aatctacttt
      901 accttcattg cttaaattta cactatcggg tgctttcgct attacacctt ctaaaactcc
      961 ttccggttca accagttcaa ctatttctac tttatctaca gttatatgct tgtctgatgc
     1021 acttttattt tttgcacctg ttactctaac tttaagtgta tgttccccat cttttaatat
     1081 cggactggta tatatcaaca cattatccga cctctctgct gcatagtaat caacaataac
     1141 ctcatctcca tcatcaatag atacagctgc tattccatgt gacggatcct ttgctccata
     1201 taatttgatc tgattaccag taaatttaac ccggaagaaa tcatcagtgc tatttgacca
     1261 gtgattatca ttcatgaaag cgcctgtttg tgccgcatat ttccagtcgc caatatattg
     1321 gaattgcccg tttgcagtac cagttacatt atcattaaca cttataatct tctcaatctt
     1381 tccgtcatag tcctcatctt catcttcatc ttcatcttca tcttcatctt catcatggtc
     1441 atctttttcg tctttatcaa tttcatattt gtctcttaaa attctggtta ttaagctgat
     1501 tgggacgcac ctgccattat tatttgccca accatatttg cttaaatcca gcttgtcacc
     1561 attgacttta atttctccgt tatccagatt aattgttatt gtggtcttat ctttctctat
     1621 agttagagtt ttgtttttct catcccattc aagttcagca ccggatgctt ttattatagg
     1681 acgtgttggg ataagaaatc ttccgcattt gattacagcc ggagctttat cttttttagc
     1741 attgccggac tgcaaaatac ctcctctatc tttctttagt ttttcaattt cattatgtaa
     1801 ttccctaacc tctttttcta attgcttaat cttgccttcc aattctttca attctttctc
     1861 tttagatact ttgctctttt cctgtaactt ttccttcact ttatccatat acttctgaat
     1921 ttctttttct ctttcatttt ttcgctgtct tagttgctcc tgtacttgct caactttcag
     1981 tgtgttattc ctgttattaa cagttagttt accaggtttc ttccagctgt ctgcaaatgc
     2041 aacagtaata ccggaagtta aaacaagagt cataattaaa acaacactta tagtttttaa
     2101 tagctttttc ataattaaaa gcctcctctc cctttcttta taattattcg taattgtctt
     2161 ataactttcc agggtatttc caacttgtaa tattattgta aaaatgccat gtacatttgt
     2221 aatttctcac atctaagtaa gaagccgttt ccgtttcagg ttgggtagcg ttttcgaaag
     2281 ctttaattca ttattattgt ttctcaatat tccagccgct attttccctt gttctttaca
     2341 ttattatttt tttcttatat tctttttacc ttggttgcct tgactatttc ctttactctc
     2401 gcccttatct ttactttcgc ccttatctat actctcgccc ttctctttgc tattggcctt
     2461 atctttgtta ttgccattat ctttgctatt gcccttatca ctattacctt tatcactatt
     2521 gcttttacct ttatcctttt tcgtattatt atctatttca tcatatacaa tttcctcatc
     2581 aaatatctca ttatcaatac tatatatatt attaatacta tctatatcat ctataccatt
     2641 tatatcattt atatcttcac caatatcttc atccatatct tcatcttcac catctatatc
     2701 ttctttgaca cccttatcca tatctttatc atcctcagtc tcatcaatat tttcattttt
     2761 cccattgctt tcttttttat ttttgctatt accgttaccg tcattaaggt tatctttgct
     2821 tttatttaca ctgctatttc cactactgcc ttttccttta ttattctttg catcattgcc
     2881 tctgtccgtt cctttatcat ttttatactc atagttcttc ccctttgcat tctcctgacc
     2941 ctttccctct gccttttcct tcactttccc attatcctta ccattatcct taccattacc
     3001 cttaccatta cccttaccct tatcatccgt ctctttctct tcttcaacct tcaatgcatc
     3061 ttccttttct tgcttcccta attctttttc ttgttccttc cctatctttt gctggattct
     3121 ctgcatctta ttttcaaata tctcttcatt ctttttttca ataactgatt tcattatttc
     3181 ccgtaccggc gcctctttcc aatcctcata tacaatttcc ggctcgctct ccttcatttt
     3241 ttcgagtaaa agatatttcc ccgaagatat tcccagattt tttgcatcat catgtttttt
     3301 tacttctaca ttttctatat agatgcttgt atcgatttca gatacgttaa tctccttacc
     3361 tacggcttct tctatatctt tctcaattat tccgaatttc ttaatatcct tgctggaaat
     3421 agtcaacaat agcatattgc tatattctgg tttaatgtac ccctcttcaa ccgcactttt
     3481 aattatattc ccgataccat ccctgacttt tttattccta tactgtttta aagatataag
     3541 ctttttacca tcatcattaa ttccttcaat atttattatc cggtcgaata tattggctgt
     3601 aatttccaca ctgggattta tatctacact aacatatgta tatggcatgc cataactgta
     3661 aacgccataa gtcaaaccaa aaataattgc aaatacagca gcgatagata ctatcttcct
     3721 tataatactg gtattgccca gtgtttcttt ggaaaatcca ttatattgat ttagattaat
     3781 ctcatatccc acttgaaaat tattattctt aaccttttca aattctccat cagttttcaa
     3841 aataattgca tgttttcctg caatctctac taccagacct ttcataacaa ctatcattcc
     3901 tttcgctgtg ctaaaaggca caaaatacca ataaaagcgt acacactttg taataactct
     3961 ttcaagctat catatgccgg attctatctt gtcttgctat aaagagttag tattaatctt
     4021 attaaggtat gtaatccctt acatactctc ttatgtaatg atagtctcct gtaattacag
     4081 tgactatagc tattatatat tttcgaagtc tctcaatttt tttccgggat acttttaaag
     4141 tcttttctat ttctgcaata ggtatatatt ttttagtttt aataacttct ataagttccg
     4201 gttttgaaat aataaatttt actatattcc tgcatgtctc gcgtgtatcc gaatgctttg
     4261 gagatgccat aaccaaatca ttaaatgtaa tatcccattt tagcagttcc ttcttaagct
     4321 cctctatttc tagtcttcta tactcactga tctcttcatt attatatttt tcaatggctt
     4381 cctcagtgct taaatctact tcttctccac tttcttcaat aaaatattca ttcagagata
     4441 ttacattcat atgttttttt tcttttctat aaaaatctat taatcgtctt ctaattactc
     4501 cctgggcaaa ggaaagaaaa ttccctttag acgaatcaaa agatttaatg ctttcaacga
     4561 aagcaataag agctatactc aattcatcac tacttccata tgttacatat tttccggtga
     4621 acttttcagt acatgatgca ataaaaggct tgtattcttc tacaaaccta ttaatctctt
     4681 catcattatc ctttcccttt atatcttcta ctctttcatt aatcgaagca tacttcatta
     4741 ttttgcctcc ggaatatgta ttaccttgag ttttattaaa taaaaccgga tacagaaata
     4801 attatatcat tagaataatg gatatttata tatatttttt gttacaaaat aagacttgct
     4861 ttgcttacta cctcagaata gtagcttgct tataactcag aaatcagagc atttttatat
     4921 agaactctgc atattgctat acaacaaaaa atccggggta atgtaaaatc cccggataaa
     4981 ttgcttttga ataattatta tttattatta actatagttt tgtacttttt gttgaaggtc
     5041 tcaaatcagc tactttcatg tttagacata attggcttaa atcaggtttt ataataaaaa
     5101 tgcaaaactt cagtattagt ttaatttgaa ttattttttt ccatatagag gtccaaaatt
     5161 catacatgct ctataatctg ctccctcaag gtcattagtt ccgaaagctg accatgtact
     5221 tggcctgaat atcctatctt tagagacatt gtgcatacat acagggattc tcaacataga
     5281 agccagagta ataagttctg cgcctatatg tccatagctg aatgctccat ggtttgcacc
     5341 ccagttagcc atcactgagt aaacatccaa aaatggtccg ttccctgtta gtattggagc
     5401 aaaccaggtt gtaggccatg ttggatctgt tcggttatca agtatttcat gcatcttttc
     5461 aggcagatca acagaatagc cttctgcaat ctggagtaca ggcccaagac ctttaattga
     5521 attcactctt gacatggtta taggcatacc accctttgta aggaaatcag atgaaaatcc
     5581 tccacccctg aaatatccaa cacttgcagg acgccacaat gtagcatcca gacattttgc
     5641 agcctcctcc ggagtaattt cccaaaaagg tttcatggca ggtttcccat ctttagtttg
     5701 ttctccg
//
LOCUS       DAOSDD010000262         5683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5079_length_5683_cov_4.948827, whole genome shotgun sequence.
ACCESSION   DAOSDD010000262 DAOSDD010000000
VERSION     DAOSDD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5079_length_5683_cov_4.948827"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK733_16940"
     CDS             complement(<1..226)
                     /locus_tag="PK733_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HOJ12257.1"
                     /translation="MVNNVIKLSFTVIGAVTGFTITRIIFTMQGINIPQDIKFAIYIV
                     VVLVTAILFYSTATKIIDSLLNFLNKLDYTI"
     gene            complement(340..819)
                     /locus_tag="PK733_16945"
     CDS             complement(340..819)
                     /locus_tag="PK733_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOJ12258.1"
                     /translation="MFNIGDKIVYPMHGAGIIESIEEKEILGNRQNYYIVRIPVGDMK
                     VMIPTENVDDIGIREVIGNDEVEKVFDILRDQTVNETSNWNKRYRENMMKIKSGNIFE
                     VTKVVKSLMIRDKEKGLSTGEKKMLNNAKQILISELVLAKGITQRELENLIEYNLHL"
     gene            complement(1391..2767)
                     /gene="radA"
                     /locus_tag="PK733_16950"
     CDS             complement(1391..2767)
                     /gene="radA"
                     /locus_tag="PK733_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ12259.1"
                     /translation="MKKGKSIFICQDCGYESQGWLGKCPACGQWNTFVEEIINIAPKG
                     GRSSVDGAVGRENKPVSLSDIEIEKEDRCSTGIKELDRVLGGGIVKGSLVLVGGDPGI
                     GKSTLLLQVCEKATVDKKILYVSGEESIRQIKLRADRLNIRNPDILMVSENNLDIIQK
                     IIQTEIPGLVIIDSIQTVYNDEMSSAPGTVSQVREATGTLMRTAKTLNIPILIVGHVT
                     KEGAIAGPKVLEHMVDTVLYFEGERHLSYRVLRSVKNRFGSTNEIGIFEMKDTGLEEV
                     ENPSMTMLSGRPENVPGSVVVSSLEGTRPMLVEVQALVSPTSFGMARRMATGVDYNRV
                     TLLMAVLEKRAGLHLQNHDAYVNVVGGLKIDEPACDLGIIAAIASSFKNKPVDAGTVL
                     IGEVGLTGEVRTVSRIEQRVNEAMRIGFKTCVIPSGNMKILEQSTISKKIEIRKVENV
                     QNVLEMLM"
     gene            complement(2925..4853)
                     /locus_tag="PK733_16955"
     CDS             complement(2925..4853)
                     /locus_tag="PK733_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOJ12260.1"
                     /translation="MLGCLSDRGELVRLFWPNIDYPQHIEKFKTGIFFTDRRYSTLWL
                     DEDGWECKQYYMGYTNILMTEYYNESVGITVKQKDFVLPDRDVLVRLYEIENNRDKWE
                     NRGANPDSKSCELGFIAYSSFVSNNQDLRGALFDFENEALVHYRHGYYFSIFSQRFVH
                     KFQLGNNPLEAARSTELYGGDSTGMMPDGALSWKLGEINPGGRKSLAVFICASQSLKQ
                     LNKLTSAIRNSDVECEFEKVKKYWNDFVAGAKHVNTGNEEIDNLYKRSLLVFKLMADK
                     NTGGLLAAPEIDEHFTKCGRYAYCWGRDAAFITGALDRCGLTETVDKFYEWALNTQDE
                     NGSWHQRYHMDGNLAPSWGLQIDETGTLIWGILQHYYVTRESGFLGKMWESVEKAVDF
                     LVSFIDEETGLPKPSFDLWEERFGEHTYSSAAVYGGISAGSEIAKILGVSWKIIENWE
                     KTAASIKYSVIKNLWKEEYGRFLRSIRTKLNPWGEEYTNDRILIQVNDKGFCKDVTPE
                     DGTIDVSLLGVCVPFNMLGIDDAMAASTVEAVEKHLAYSGCGGIGRYQYDNYIGGNPW
                     VIATLWVALYHVRKGNLQRAGEYLEWAVSSRTELDLLPEQVDKNTGKPAWVIPLTWSH
                     AMFVLLLFELIEAGYFSG"
     gene            5192..>5683
                     /locus_tag="PK733_16960"
     CDS             5192..>5683
                     /locus_tag="PK733_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ12261.1"
                     /translation="MVIDFNKIIFNLTQVMQVLIFTVGCYFFSISIFGWMKGKKELTD
                     KIPPKKKFALVIAAHNEELVIAHIIDSLFRQSYPKEFFDVFVIADNCTDMTAEIAEGH
                     GAKVFVRENDTERGKGYALEWMFNKIFTMEEKYDAISVFDADNLVSSNYLYEMNKQLS
                     KGYK"
BASE COUNT         1709 a         1286 c          807 g         1881 t
ORIGIN      
        1 gtatcgtgta gtcaagttta ttaaggaaat tcaacaaaga gtcaataatt ttcgtagctg
       61 tagaataaaa taaaatggca gtaaccagta ccacaacaat ataaattgca aatttaatgt
      121 cctgcggtat atttattccc tgcatagtga aaataattct tgtaatagta aaaccggtaa
      181 cagctcctat aacagtaaag gaaagtttta taacattatt taccacaata ccttactccc
      241 ctgtttataa atttatatgc actttatatt ctatatccct cattgccaat atattctaac
      301 ccatatttat gaacttttgt taaatggact acatttcact tatagatgta aattatattc
      361 aataagattt tccaattctc tttgagtaat acctttagct aaaaccaatt cgcttattaa
      421 tatttgcttt gcattattca gcattttttt ttctccagta gaaagtcctt tttctttatc
      481 ccttatcata agcgatttta ctactttagt tacttcaaat atattgccac ttttaatttt
      541 catcatgttt tctctgtatc tcttattcca gttgcttgtt tcattaaccg tctgatctct
      601 taatatgtca aaaacctttt ctacctcgtc attccctatc acttctctta tacctatatc
      661 atcaacattc tctgtaggta tcattacttt catatctcct acaggtattc gtacaatata
      721 atagttctgt ctattgccca gaatttcctt ttcttctatt gattcaatta tacctgctcc
      781 gtgcattggg tacacaatct tgtcccctat gttaaacatt acaaattccc ccgtatatct
      841 cagcgattta taccgctaat tttgaaaagc gtacttgttg taccaagaaa attcgcttcg
      901 ttcatgactg aattaagcaa tgatgcgaat tttcttgttg ctagggaacc ctaagttccc
      961 ttcgacagat tctgcttttt ttacttattg cagaattctg agcaccaaag cgttttgcaa
     1021 agcaaaatcg ctgctcgctc tgcaatgccc ttctaccctt aaagcgggaa ggggagtcct
     1081 ctttccaaac cccagtgtag ggattttttt gaaattgtat aaattcctgt actttaaaag
     1141 attccacaca aaacaactcc atcagctttg ctaaacccaa ggcaagtcaa atcttccagt
     1201 ataacaacct atgaatttaa tataagtatt tataataatt taatataagt atctataata
     1261 aataaatata gacacctgta aaattaatat aaatacctat tagcctataa atatagtata
     1321 gtaaatattt gctatataat taagtatact acaaaggaat tatgttgtca aagatattag
     1381 ccaggttaca ttacatcaac atttccagca cattctggac attttccacc tttctaatct
     1441 ctattttctt actaatggtg ctttgctcca gtattttcat attgcctgag gggattacgc
     1501 atgttttgaa tcctattctc atagcttcat tcaccctctg ttctattctg ctaactgtcc
     1561 ttacttctcc cgttaagccg acttcaccaa ttaaaactgt ccctgcatct acaggtttat
     1621 ttttaaaact tgaagctata gcagcaatta tgcccagatc acatgcaggt tcgtcaattt
     1681 ttaatccccc tacaacattt acataagcgt cgtgattttg aagatgcaga cctgctcttt
     1741 tttccagtac tgccattaaa agagtaaccc tgttataatc tactcctgtt gccatcctcc
     1801 ttgccatgcc aaaactggta ggacttacaa gggcctggac ttccaccagc attggtcttg
     1861 taccctcaag acttgataca acaacagatc cgggaacatt ttccggacgc cctgacaaca
     1921 ttgtcattga aggattttca acttcctcaa gtcctgtatc cttcatttca aaaataccta
     1981 tttcatttgt tgagccaaac ctgtttttaa ccgaccttaa tactctgtaa ctaaggtgtc
     2041 tttccccttc aaagtacaaa acagtatcta ccatgtgttc aaggacttta ggccctgcaa
     2101 ttgcaccctc ctttgtaaca tgacctacaa tcaatatggg aatattgaga gttttcgccg
     2161 ttctcatcaa tgtgccggtt gcttccctta cctggcttac agtacctggt gcagaagaca
     2221 tttcatcatt atatactgtt tgtatggaat caataataac cagccctggg atttccgttt
     2281 gaatgatttt ttgaataata tccagattgt tttcagatac cattaaaata tccggatttc
     2341 taatattcaa tctatcagct cttaacttta tctgtcttat ggattcttct ccggatacat
     2401 ataatatctt tttatctaca gtggctttct cacacacctg aagcagtaat gtggatttgc
     2461 caataccagg atccccacct accaatacca gggaaccttt aacaataccc ccgcccagca
     2521 ctctgtccaa ttcttttatt cctgtactgc atctatcctc tttttcaatc tcaatatcac
     2581 tcaaactaac cggcttgttt tcccttccga cagcgccatc aacagaactt ctgccgcctt
     2641 tgggggcaat gttaataatt tcttctacaa aggtgttcca ctgcccgcag gcagggcact
     2701 tacccagcca accctggctt tcatatccac aatcctggca tataaaaata gactttcctt
     2761 ttttcaatag tatcaacccc ttcactcaga tacggggcaa ctcttcaaca taaaagggat
     2821 atctcttttt aagagaagag tttcccccgc ccagttgcag cacctccact actcaccact
     2881 cactatccac cacccactac tcactatcca ctacccacta ctcactatcc actaaaatac
     2941 cctgcctcta tgagttcaaa aagcaacagc acaaacatgg catgggacca ggtcagagga
     3001 ataacccatg caggtttccc cgtattttta tcaacctgtt caggcagaag atcaagctcc
     3061 gtacgtgagc tcactgccca ctccaaatac tctcctgccc tttgcagatt accttttcgg
     3121 acatggtaga gtgctaccca taatgtagca ataacccagg ggtttccccc aatataatta
     3181 tcgtattggt atcggcctat tcctccgcac ccgctatatg caagatgctt ttccacagct
     3241 tcaactgttg aagctgccat ggcatcatca atgcccaaca tattaaaagg tacacaaaca
     3301 cctaaaaggc tcacatctat agttccgtct tcaggagtta catccttgca gaagccctta
     3361 tcattaacct gaataagtat tctgtcattg gtatattcct ctccccaggg attaagttta
     3421 gtccttatac tccttaaaaa gcggccatac tcttccttcc atagattttt gattactgag
     3481 tattttatag atgctgccgt cttttcccag ttctcgataa ttttccatga cactccaaga
     3541 atttttgcaa tttcagatcc tgcagatatt ccgccatata cagcagcgga agaataggta
     3601 tgctccccaa acctctcttc ccagagatcg aaacttggtt taggcaatcc cgtttcttca
     3661 tcaataaaag aaaccaaaaa atcaactgcc ttctctacac tctcccacat tttccctaaa
     3721 aagcctgatt cccttgttac atagtaatgt tgaagaattc cccagattaa agtacctgtt
     3781 tcatctatct gaagtcccca ggatggagca agatttccat ccatatgata cctttgatgc
     3841 catgagccat tttcatcctg agtatttaaa gcccattcat aaaatttgtc cacagtttcg
     3901 gtcaatccgc atctgtcaag tgctcctgta ataaaagcag catctcttcc ccaacaataa
     3961 gcatatcttc cacatttagt gaagtgttca tcgatttccg gagcagctag caatccacct
     4021 gtatttttat ctgccataag cttaaaaacc agaagagacc gcttatacaa gttgtcaatt
     4081 tcttcatttc ctgtatttac atgctttgca cccgcaacaa agtcattcca atatttttta
     4141 actttctcaa attcacattc gacatctgaa tttctaattg cacttgtgag tttatttagc
     4201 tgttttaaag attgtgaggc acaaataaat acggcaaggg attttctacc tccggggttt
     4261 atttctccca acttccagga aagagcacca tccggcatca tacctgttga atcaccccca
     4321 tatagttccg tagatctggc agcttccagt ggattattcc ccagttggaa cttatgcaca
     4381 aacctttggg agaatattga aaagtaatac ccatgcctgt aatgaaccaa tgcttcattc
     4441 tcaaaatcaa ataaagcacc tcttaaatcc tggttattac ttacaaaaga agagtatgca
     4501 ataaagccaa gttcacagct tttactatcc gggttagctc ctctgttttc ccatttatca
     4561 cggttatttt caatctcata taatcttacc aatacatctc tatccggcaa aacaaaatcc
     4621 ttctgtttta ctgtaattcc aacactctcg ttatagtatt ccgtcataag aatatttgta
     4681 tatcccatat aatattgctt gcattcccag ccatcttcat ccagccacaa agtactgtat
     4741 cttctatctg taaagaaaat gccggtttta aatttttcaa tatgctgcgg atagtcaata
     4801 ttgggccaga atagccttac aagctcgcct ctatccgaca gacaaccaag catgctggaa
     4861 tttccaacta ttgcattgtt aaaatacgtt tttataaaaa tcacccacca tgatctgagt
     4921 cagcagcaag tcaccaaaat cggatttttc tttacgagga aacatatccc aaaagacatg
     4981 gttacaaaac ttacccactg ctctattctt agttattata cttgtttttc atataaaagt
     5041 ccatttactg taattttttt tacacaatac aataaatatt ttaactcata aagcacaaaa
     5101 tataataaag tataaagtta tttcttttaa atcaaatata ttatttatat ttggcattaa
     5161 tcgtaaaaat atgtatttat ggaggaatta aatggtaatt gactttaata aaataatttt
     5221 taatcttacc caggtaatgc aagttttaat tttcactgtt ggatgctatt tcttcagcat
     5281 atccatattt ggatggatga aaggaaaaaa ggagctaact gataaaattc ctccaaagaa
     5341 aaaatttgct cttgtaatag cggctcataa tgaagaacta gttatagctc atattattga
     5401 ttctctattc cggcaaagtt atcctaaaga gttttttgat gttttcgtaa tagctgataa
     5461 ttgtaccgat atgacggcag aaatagcaga aggtcacggt gccaaagttt ttgtacgtga
     5521 aaatgataca gaaaggggta aaggttatgc cttggaatgg atgtttaata aaatattcac
     5581 aatggaagaa aaatatgatg ctataagtgt atttgacgcg gataatcttg tatcatcaaa
     5641 ctatctatat gaaatgaata agcaactaag taaaggttat aag
//
LOCUS       DAOSDD010000263         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5129_length_5633_cov_5.188777, whole genome shotgun sequence.
ACCESSION   DAOSDD010000263 DAOSDD010000000
VERSION     DAOSDD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5129_length_5633_cov_5.188777"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PK733_16965"
     CDS             complement(<1..1255)
                     /locus_tag="PK733_16965"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ12262.1"
                     /translation="MRISQSFIPTLREVPAEAEIVSHQLMLRAGLMRKLASGVYAFLP
                     MGYRAFRKIEQIMREEMDRAGAQELVMSSILPSEYYQASGRWEVFGPEMFKFKDRNGR
                     DFCLGPTHEEIFTEIVKNEVRSYRALPLILYQIQTKFRDERRPRFGVIRGREFVMKDA
                     YSFDRDNEGLDLSYQKMHDAYCRIFDRCCLDYIVVDADSGAMGGSGSQEFMVKSAIGE
                     DVIAYCEECGYAANEEKAECVPEACCESCSGMCCGQLPVEKVPTPDVRTIEELMKFFN
                     CSPKEFAKTLIYRADDKYVAALVRGDRELNETKLQNYLGCVELEMADTEAVKRITNAD
                     VGFAGPIGLRVDIIVDPEVENMKNFVVGANETGYHFKNVSIFRDFKPTVIKDIRNIAP
                     GDKCPKCGASVKISRGIEVGHIFKLG"
     gene            1470..1973
                     /gene="ispF"
                     /locus_tag="PK733_16970"
     CDS             1470..1973
                     /gene="ispF"
                     /locus_tag="PK733_16970"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOJ12263.1"
                     /translation="MKVGIGQDSHRFDFDDKEKKLILGGIIFEGYPPLKGNSDADVVL
                     HSLTNAISGVTCVNILGEISDKMCFEENITDSSAYLKEALKYMGDNKIVHVSISVECQ
                     RPKISPKIPEMRRRLSELLRISESCIGITATTGEDLTEFGKGNGIQVFSSVVVVPGIV
                     RIKPDYI"
     gene            complement(2037..3251)
                     /locus_tag="PK733_16975"
     CDS             complement(2037..3251)
                     /locus_tag="PK733_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ12264.1"
                     /translation="MKAKHVLTVFKKEVKDILRDKKTIISSILLPMVLIPFMNILVGG
                     GVQKLQKDITENISIALAQNSKTEEIRELVNNEIIGNDPNIALIDIDDPIEAIKKDKV
                     RVVLEFEEGYAEKLKDNKPFSIKILYDNTKAKSEGSLSIVRNLIDDFNNRLVKERLLK
                     QGINPEILEPAKIELNNIADSKSGGNFALSMMLPFMVAILVAAGGIPAATDLIAGEKE
                     RNTFEPLLATKPDRASILAGKYLTVILFSFVSVIAIMIGFAIGYIINPNSVTMGTGEQ
                     LGGFNIPVPAILLSLLLTITMGVTFAGIQIALSTYARSFKEAQTYLSFLMFAAMIPGY
                     MTIFMQASDIKTYMFLIPVLNTISAFKMVLGGAINYTNLILALVSSVIYTMITLALAV
                     AMFKREKVLFRS"
     gene            complement(3288..4022)
                     /locus_tag="PK733_16980"
     CDS             complement(3288..4022)
                     /locus_tag="PK733_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ12265.1"
                     /translation="MLKLENLTKAFGDAKAVAVNNLTFEVGKGEIFGLLGENGAGKTT
                     TLRMMATMLKPTSGTGKVSGFDIIQQPEKVRSCIGILFGGESGLYDRLTAAENIAYFG
                     ELYDMDIKYINERIAYLSKIFGMEEYIDRRASRLSKGMRQKTAFARAIVHNPDVMLFD
                     EPTSGLDVGAIREVHDFIRDCGKNGKTIILSSHSMGEVEKLCDRIGIIHKGNLVDIGT
                     IDQLKEKYNNNNLEEIFIKITGRDNK"
     gene            complement(4077..4796)
                     /gene="ispD"
                     /locus_tag="PK733_16985"
     CDS             complement(4077..4796)
                     /gene="ispD"
                     /locus_tag="PK733_16985"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOJ12266.1"
                     /translation="MKDVKLKKSWKGIVSVVIAAAGKGTRMNLDINKQYIEICRMPVL
                     ARTIEAFQECRFIDEIVLVINQNDLVYCKKNIIDAYGFTKVGKIVAGGDKRQQSVFKG
                     LNEVSRDCEIVLIHDGARPFIREDVILESIAEAKDFGASCVAVPVKDTIKRSGSEGFF
                     EETLQREGLWSIQTPQTFKYEIIKDAHEQAVKDGFEGTDDSVLVERLGLPVKIVMGSY
                     DNIKITTQEDLAIGEAIIKGM"
     gene            complement(4798..>5633)
                     /locus_tag="PK733_16990"
     CDS             complement(4798..>5633)
                     /locus_tag="PK733_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOJ12267.1"
                     /translation="TIQNMTLYELVISSAGLVIGLIIANLIAIPINKLNIVGVPLSIT
                     INILFGTAGILITTSKKNDPLPGIKNSGKLAEPKVVDTSVIIDGRIVDIYRTGFLEGE
                     LVVPAFVLEELQHIADSSDPLKRNRGRRGLDVLNMLQKELGCPVRIENIALPEGTEVD
                     TELLRVAQKFGAKILTIDYNLNKVAGVHGVSVLNINELSNAVKPIALPGEEMVVQVIK
                     DGKENGQGIGYLDDGTMIVVDGGRRHIGENISVMVTSVLQTAAGRMIFAKPKYTVERM
                     L"
BASE COUNT         1655 a         1243 c          902 g         1833 t
ORIGIN      
        1 tgccaagctt gaaaatatgg cccacttcta taccacggga aattttaacc gaggcaccgc
       61 atttagggca cttatcacct ggagcaatat ttcttatatc tttaattaca gtgggtttga
      121 aatccctgaa tatgctcaca ttcttgaaat gatatccggt ttcattagct ccgactacga
      181 aatttttcat attctcaact tcagggtcaa caataatatc aaccctcagt cctatcggtc
      241 ctgcaaaccc aacatcagca ttagttattc tttttactgc ttccgtatct gccatttcca
      301 gctcaacaca tcccagataa ttctgaagtt tggtttcatt tagttcccgg tcccctctta
      361 ccaacgctgc aacatatttg tcgtctgctc tgtaaatcaa tgtttttgca aactccttgg
      421 gtgaacagtt aaagaatttc attaactctt ctattgttct tacatcagga gtcggcacct
      481 tttcaacagg cagctgcccg cagcacatac cactgcagct ttcacagcag gcttcaggta
      541 cacattccgc cttctcctcg tttgctgcat atccacactc ttcacaataa gcaataacat
      601 cctcaccgat tgcggattta accataaact cttgtgaacc tgaacctccc attgcaccgg
      661 agtcagcatc aactacaata tagtcaaggc agcagcgatc aaaaattctg caatatgcat
      721 catgcatctt ttggtacgac aagtcaaggc cttcattgtc tctatcaaag ctgtaggcat
      781 ccttcattac aaactctctg cctcttataa caccaaatct tggccttctc tcatctctga
      841 actttgtttg aatctgatat agtataagtg gaagagctct gtaagagcgg acttcatttt
      901 taactatttc agtaaatatt tcctcgtggg tgggccccag gcaaaagtct cttccatttc
      961 tgtctttgaa tttgaacatt tcaggtccaa aaacttccca ccgtcctgaa gcctgataat
     1021 attccgaagg aagaattgaa gacattacca gttcttgagc tcctgcccta tccatctctt
     1081 cacgcattat ctgctcaatt ttgcggaaag ccctgtaacc cataggtaaa aaggcatata
     1141 cacctgatgc aagtttccgc atcaaacctg ctctcagcat aagctgatgg cttactatct
     1201 ctgcctctgc agggacttct ctcaatgttg gtataaaaga ttgtgatatt ctcataatta
     1261 ttaatacctc gtccttttgc gaatgatata caaaaaatat acaatacaag cataacaaag
     1321 tcaagatgca gtgagcactg ttcctggtac atattgaaac atttcctcta aactgataga
     1381 cttgagtaaa taataattgt gtaaataaaa catatcttaa taagaaggat ggtaactata
     1441 tgaatggcaa ctatatgaat ggcgactgta ttaaggttgg aatcgggcag gatagccaca
     1501 gatttgattt tgatgataag gaaaaaaagt taattttagg tgggataatt tttgaaggat
     1561 atccgccgct gaaaggaaac agcgacgcag atgtggtgct tcattctcta acaaatgcaa
     1621 tttcaggggt tacctgtgtt aatatactag gggaaataag cgataaaatg tgctttgaag
     1681 aaaatattac tgatagctca gcatatctta aagaagcctt gaaatatatg ggagataata
     1741 aaattgtcca tgtatctatt tccgtagaat gtcaaagacc taaaatatct cctaaaatac
     1801 ctgaaatgag aagaaggctt tcagaactcc ttagaatatc agaaagctgt ataggaataa
     1861 ctgctacaac cggtgaggac cttacagaat tcggcaaagg caacggtatt caggttttca
     1921 gcagcgtggt tgttgtaccc ggcatcgtcc gaatcaagcc ggattatatc tagtgggcgc
     1981 atattattca caaatgcgcc catatctaat aacgccaaat aatgtcattt gttattctaa
     2041 cttctgaaaa ggaccttctc ccttttaaac atagctactg caagagccag ggtaatcatt
     2101 gtataaatta cagatgatac aagagccaat ataaggttgg tatagtttat tgcccctccc
     2161 aggaccattt taaatgcgga aatagtattt aacacaggta tcagaaacat atatgtttta
     2221 atatcgctgg cctgcataaa gatggtcata tatcccggta tcatggctgc aaacattaaa
     2281 aaggacagat atgtttgcgc ttccttaaag gatcttgcgt aagtacttaa tgcaatctgt
     2341 attcctgcaa aagtaacccc catagtgata gttagaagaa gggaaagcag aattgcagga
     2401 acaggtatat taaaacctcc cagttgttct ccggttccca ttgtcacaga gttgggatta
     2461 ataatatatc caatggcaaa gcctatcata attgctatta cacttacaaa tgaaaagagt
     2521 attacagtga gatattttcc tgcgaggatt gatgccctgt cgggttttgt agccagcagt
     2581 ggttcaaaag tgtttctctc cttctcccct gcaataagat cggttgcagc tggtattccg
     2641 ccggcagcta caagtatggc taccataaac ggcagcatca ttgataaggc aaaattgcct
     2701 ccgctttttg aatcagcaat attgtttaat tctatttttg caggctcaag tatttccgga
     2761 tttatcccct gtttcaataa tctttctttc acaagcctgt tattaaagtc atctatcaaa
     2821 tttcttacta ttgaaaggct tccttcagat ttcgctttag tattatcata taatattttt
     2881 attgaaaaag gcttattatc ttttagtttc tctgcataac cttcctcaaa ctcaagcaca
     2941 actcttactt tatccttctt gatagcttca attgggtcat caatatcgat taaagctatg
     3001 ttggggtcat taccaattat ttcattgttt accaactctc ttatttcttc agttttcgaa
     3061 ttctgagcaa gtgcaatact aatattttca gttatgtcct tttgtaattt ctgaactcct
     3121 cctcctacaa ggatattcat aaacggtatt aaaaccattg gtaagagaat actggaaatg
     3181 atggtttttt tatctctcaa tatatcttta acttcttttt taaatacagt taatacatgc
     3241 ttcgctttca ttttcggcct ccgtccaact ataagtccaa ctatagttta tttattatct
     3301 ctgcctgtaa tcttaatgaa tatttcttcc agattattgt tattatattt ttctttcaac
     3361 tggtcaatag taccaatatc aacgaggttc cctttgtgaa taatgcctat tctatcacat
     3421 aatttttcaa cttcacccat tgaatggctt gaaagaatta ttgttttgcc atttttacca
     3481 caatcacgaa taaagtcgtg aacctcacgg attgcaccca catcgagacc tgaggtgggt
     3541 tcgtcaaata acattacatc aggattatgt acaatagctc ttgcaaaagc cgttttctgc
     3601 ctcataccct ttgaaagtcg ggatgctctc ctgtcaatat attcctccat gccaaatatt
     3661 ttgctaaggt acgcaatcct ttcatttata tacttaatat ccatatcata caactcgcca
     3721 aagtatgcta tattttccgc tgcagtaaga cggtcataaa gcccgctttc acctccaaac
     3781 aggataccga tgcagctccg caccttttcg ggttgctgta ttatatcaaa tcctgaaact
     3841 ttgcctgttc cggaagttgg cttgagcata gtagccatca ttctcaaagt agtagttttg
     3901 cctgctccat tttcaccaag cagaccaaat atttcacctt ttccaacttc aaatgttaaa
     3961 ttattaacag caacagcttt ggcatctcca aaagcctttg tcaaattttc aagtttaagc
     4021 atttaatcac tccaatctaa agtaagaggt attccttttg tattaggcat gtcacttcac
     4081 attcccttaa ttattgcttc tccaatagcc agatcctctt gtgtggtaat ttttatatta
     4141 tcataacttc ccataacaat tttcacaggc aatcccagtc tttccaccaa tactgaatca
     4201 tctgtacctt caaagccgtc ttttacagct tgttcatgtg catctttaat tatttcatat
     4261 ttaaaagtct gaggtgtttg aatagaccat aaaccttccc tttgaagggt ttcctcgaaa
     4321 aagccttcag atccggaacg tttgattgta tcttttacgg gaactgcaac acaggaagct
     4381 ccaaaatctt tagcttcagc tatgctttcc agaattacat cttctctgat aaaaggcctt
     4441 gcaccatcat gtattaatac tatttcacaa tcccgggaca cctcattcag tcctttaaat
     4501 accgattgct gccttttatc accgcctgca actattttgc caaccttggt aaatccatat
     4561 gcatcaatta tatttttttt acaatagaca agatcatttt gattaatcac tagaactatt
     4621 tcgtctataa atctgcattc ctgaaaagct tcaatagttc ttgcaagaac aggcatgcgg
     4681 caaatctcta tatactgctt gttaatatca aggttcatgc gtgtaccttt gccagctgct
     4741 gcaataacaa cgcttacaat acctttccag gattttttaa gttttacgtc tttcaagcta
     4801 aagcatcctt tcaacagtat atttaggctt tgcaaaaatc attctacctg cagcggtttg
     4861 caatacactt gttaccatta cactgatatt ctctccaata tgccttcttc caccatctac
     4921 tacaatcatt gtaccatcat caagatagcc tattccctgt ccattttctt tcccatcctt
     4981 aattacctgg acaaccatct cttcacccgg taaggcaata ggttttacgg catttgataa
     5041 ttcatttata tttaaaacac ttacaccatg tacgcctgca actttattta aattgtagtc
     5101 aattgtcaat attttagctc caaatttctg agcaactctc aaaagctcag tatcaacttc
     5161 ggtaccttct ggaagagcaa tattttcgat ccttacagga catcccaatt ccttctgaag
     5221 catgttaaga acatccagac cccttctccc ccggtttctt ttaagcggat cagaagagtc
     5281 tgcaatatgc tgaagttctt ccagcacaaa ggcaggcacc accaattcgc cctctaaaaa
     5341 accagtccgg tatatgtcaa ctattctgcc atcaattatg acacttgtat caacaacttt
     5401 tggttcagca agtttgccgc tgtttttaat acccggcaat ggatcattct tttttgaagt
     5461 ggtgattaat atacctgcag taccaaataa aatattaata gtaatggata atggaacgcc
     5521 aacaatattc agcttgttaa ttggtattgc tataaggttg gcaataatga ggcctatcac
     5581 caagcctgca gaactgataa ccagttcgta caacgtcatg ttttgtatcg tgt
//
LOCUS       DAOSDD010000264         5624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5144_length_5624_cov_4.184414, whole genome shotgun sequence.
ACCESSION   DAOSDD010000264 DAOSDD010000000
VERSION     DAOSDD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_5624_cov_4.184414"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..150)
                     /locus_tag="PK733_16995"
     tRNA            complement(76..150)
                     /locus_tag="PK733_16995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Glu,seq:ttc)
     gene            733..1206
                     /locus_tag="PK733_17000"
     CDS             733..1206
                     /locus_tag="PK733_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOJ12268.1"
                     /translation="MYYNISNDRMLESLNSHVKKVREGKEMLINEYYREETEESIKLS
                     IFLKEYVSSLNNYINSTKSNNESGMIQPVVTIGSIVTVKDLDDNKIYRYQIVLPFLTN
                     LNSDHDDASCLSPLGKALLLKTIDEKVEIEIPAGTLRYVVKSITPPESFIPAYNK"
     gene            1328..1403
                     /locus_tag="PK733_17005"
     tRNA            1328..1403
                     /locus_tag="PK733_17005"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1361..1363,aa:Lys,seq:ttt)
     gene            complement(1478..1687)
                     /locus_tag="PK733_17010"
     CDS             complement(1478..1687)
                     /locus_tag="PK733_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOJ12269.1"
                     /translation="MPKITSDMIVSEVLKLDKGTVPIFLNNGLYCLGCPSASGESIKD
                     ACEVHGLDADKLVRELNEFFESIQG"
     gene            complement(1872..2423)
                     /locus_tag="PK733_17015"
     CDS             complement(1872..2423)
                     /locus_tag="PK733_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOJ12270.1"
                     /translation="MKHIIYTPLEKDMISQLKTGDEVTINGTVYTARDAAHKRLVAMV
                     QEGKQLPFDIEGQIIYYVGPCPAKPGKVIGSAGPTTSGRMDAYAPLLISMGLAGMIGK
                     GQRNKDVVDAIIKYKSIYFGAIGGAGALISEKIVSQEIVAFPELGPEAIRKLEVRDFP
                     AVVVIDSYGNNLYEIGREKYRMP"
     gene            complement(2481..3323)
                     /locus_tag="PK733_17020"
     CDS             complement(2481..3323)
                     /locus_tag="PK733_17020"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOJ12271.1"
                     /translation="MRVIDASLITDTVKNLCIASNYCLNHDIFKALQDGLSIEESDTG
                     KGILKKIIENANLAETEGMAICQDTGMAVFFVDIGQDIHISGGNLTEAINEGVRRGYK
                     EGFLRKSVVGDPIERVNTGDNTPAIIHYNIVEGDKMRITIAPKGFGSENMSALKMLKP
                     SDGIEGVKKFVVETVDKAGPNPCPPIVVGVGIGGTMEKAAILAKKALTRSVDVRNNIE
                     YVKNLELELLEKINKLGIGPAGLGGRITALAVNVEVFPTHIAGLPVAVNISCHATRHS
                     EAII"
     gene            3721..4101
                     /locus_tag="PK733_17025"
     CDS             3721..4101
                     /locus_tag="PK733_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HOJ12272.1"
                     /translation="MNPVKNILVCVTQQKTCERLIIKAASLRDEFKGELYVIHVTKNG
                     LNFLDNPKEGEALQYLFEISKSVGANLSVLKSDNIIKCIADFAKDNNINYIILGESPK
                     DTDDNIFHKKLRNLLHNIELEVIP"
     gene            4265..4537
                     /locus_tag="PK733_17030"
     CDS             4265..4537
                     /locus_tag="PK733_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ12273.1"
                     /translation="MNKTELVNSISAKTGLSKKNSEAALNAFVSSVEESLQNGEKVVL
                     VGFGTFEVRERAERKGRNPQTKEEIVIPASKAPVFKAGKGLKDLVK"
     gene            complement(4616..5089)
                     /locus_tag="PK733_17035"
     CDS             complement(4616..5089)
                     /locus_tag="PK733_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ12274.1"
                     /translation="MPSSGKSTIGSNLADILNMNYIDTDNLILESQKKDLRDIVSLYG
                     HHEFLKIQEEVILQLDTVNSIIATGGSVIYGDAAMVHLKKDGVVIYLQLDFNSVNSRI
                     KQGRRFAKNEEQSFIDMYHERVPLYERYSDIVIDCSGKDVKAIVKEIIEKLQKCF"
     gene            5335..5475
                     /gene="scfA"
                     /locus_tag="PK733_17040"
     CDS             5335..5475
                     /gene="scfA"
                     /locus_tag="PK733_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOJ12275.1"
                     /translation="MKHIKIINAATLKNTVKNGGCGECQTSCQSACKTSCTVGNQKCE
                     KN"
BASE COUNT         1807 a         1032 c          896 g         1889 t
ORIGIN      
        1 cctcgacctc cagcgtgaca ggctggcatt ctaaccaact gaactaccgg gccattttct
       61 ttttgctaaa agctatggtg acccataggg gactcgaacc cctgataccg ccgtgaaagg
      121 gcggtgtctt aaccgcttga ccaatgggcc atttaaagcc agcaaataat attttacaca
      181 ggtcgcagcc caatgtcaag tagttttaga gtttttttaa ttttttttga ttttataagt
      241 tttatattcc aatactactt tgctatgttg ccaatggaaa gcgcatttct ggggagtatt
      301 ttttactgac ccgtaaagaa tacatctgag agaaagacca caaggccttc tatagaggta
      361 tttctccctg cgacatcttc ccgatgctat caaaaattat cctggaagac atcgctttcc
      421 aaattggcat attagtacta ataatattag caaataaaat accggtcagc atgacattat
      481 caaagaataa tcgtaaccaa tatcatacca acattgacac tgatttacat ttgtgttagt
      541 atatatatga gttaaattac cataaaatat tgttacaatg gcttcatgat aatggcctca
      601 tggacatgaa tatatttgtc gtataggcat ttcataaaaa cttgtgagat gccttgctat
      661 atacaatcat atattccgcc aaattcgaat aatttttaat aaagatattt aaatataaaa
      721 aggaagatgt ttatgtatta caatattagt aatgatagga tgttagaatc tcttaatagt
      781 catgttaaaa aagttagaga aggtaaagaa atgctcataa atgagtacta tagagaggaa
      841 acagaagaaa gtattaaatt aagtatattt ttaaaggagt atgtatcatc attaaataat
      901 tatataaata gtacaaaatc caataatgaa tcaggtatga ttcaacctgt tgtaacaata
      961 ggaagtattg ttacggtgaa ggatctggat gataataaaa tttataggta tcaaatcgtt
     1021 ttgcctttct taactaattt gaattctgac cacgatgatg catcctgttt atcgcctttg
     1081 ggaaaggccc ttttattgaa aactattgat gaaaaggttg aaattgaaat acctgccggt
     1141 acactaagat atgttgtaaa aagtatcaca cctccggaat cttttatccc ggcatataat
     1201 aaataattgg taaatacttg attatagccc aataatcata aatattttga aaaaagtttt
     1261 aaaaacagtt gacaaaacta ctgtttttgt tgtactataa gattcggctt tgaaaataaa
     1321 atactttggg ccattagctc agtcggcaga gcacttgact tttaatcaag gtgtcccgcg
     1381 ttcgagtcgc ggatggctca ccaaaaataa agggtttcag ttttttaaat actgaagccc
     1441 tttgttttta atctatattt tgctttttgc cctctgttta tccttgtata ctctcgaaga
     1501 actcattgag ttctcttacc agcttatcgg catccagtcc atgcacttca caagcatcct
     1561 ttatactttc tccggaggct gaagggcatc ccagacaata taaaccgtta ttaaggaata
     1621 ttggtactgt acctttgtca agttttaaaa cttctgaaac tatcatatca gatgtaatct
     1681 ttggcatatt attcactccc tgtctcttgt tttacttact atttactaat acccattatt
     1741 gtataattct acccacaata ttatacgaat tatctttgca tattatatta tatgaaaatt
     1801 tataatgcaa tataattgtg ctgtaaaggg gtgcattttc taaatatgca ccctaccctg
     1861 ctaaacatga tttatggcat ccggtatttt tctcttccta tttcataaag attgttacca
     1921 tagctgtcta ttacaacaac agccgggaaa tctcttactt ccaattttct tatggcttcc
     1981 ggtccaagtt ccgggaatgc tacaatttcc tggctgacaa ttttttcaga aatcagagct
     2041 ccagctcctc caattgcgcc aaagtaaatt gacttatatt ttataatcgc atcaaccaca
     2101 tctttatttc gttgtccctt gccaatcatc cctgccagtc ccatactgat tagtaatggg
     2161 gcgtatgcat ccattctgcc actggttgta ggtcctgcag accctataac ttttcctggt
     2221 tttgcagggc atggaccgac ataatatatt atctgtcctt ctatatcaaa aggtagttgt
     2281 tttccctcct gtaccatagc taccagacgt ttatgagctg catccctggc cgtataaact
     2341 gtaccattaa tggttacctc atctccggtt ttcaactgtg atatcatatc tttctccaaa
     2401 ggagtataaa ttatatgttt catttttccc tcctgtacat tttgtaataa ttcattgtgc
     2461 tttattggac caactagcag ttatatgatc gcctctgaat gccttgttgc gtggcagcta
     2521 atatttacag ctacaggcaa ccctgctata tgagttggaa atacttcaac attaaccgcc
     2581 agagcggtaa tgcgtcctcc aagtcctgca ggccctatcc caagcttgtt aattttctca
     2641 agcagctcta attcaaggtt ttttacgtat tcaatgttat ttcttacatc tactgatctg
     2701 gttaaagctt tctttgccaa tatggccgct ttttccattg tacctcctat acctactcct
     2761 acaactattg gagggcatgg attgggccct gctttatcaa ccgtttcaac aacaaatttc
     2821 ttaacgccct caattccgtc agagggtttt aacattttta aagcactcat attttcactg
     2881 ccaaatcctt ttggggcaat agtaattctc attttgtccc cttcaacaat attatagtga
     2941 attatagcag gggtattgtc acctgtattc actctctcta taggatcccc gacaactgat
     3001 tttcgaagga aaccttcttt atatcctctt cgtacgccct catttattgc ctcagtcaga
     3061 ttccctccag agatatggat atcctgccca atatcaacaa aaaacacagc catccctgta
     3121 tcctgacaaa ttgccattcc ttcagtttca gctaaatttg cattttcgat aatttttttt
     3181 aatatgcctt tacctgtatc tgattcttcg atagaaagac catcctgtaa agctttaaat
     3241 atatcatgat ttaagcagta atttgaggct atgcacagat ttttaactgt atcggtaatt
     3301 agcgatgcat caataactct catttatacc tcccaaaaaa aatgtaatat tttatactta
     3361 aaatagtata tgactatttt aatatactgg tattggaaaa tcaacaaatt gaaatacaaa
     3421 ggggtacatt tactaaaagt gaagaaatat ttaccaggag ctgtcgttac gctgcttacc
     3481 aatattttct tcacttaaat taaatgcacc caaatataaa gcgaaacata aatgaaacaa
     3541 atattaacag atatttaata attattttga tataattaat atgtttggta ttatcaggct
     3601 gaaattataa gatttgatcg tttgatcata agatttgatt aattggaagg gagatggtgt
     3661 gcaaaataat tttccattac tatttgtgcc accagtcaaa gagatgaaat ggaggttatt
     3721 atgaacccgg taaaaaatat attggtttgt gttactcagc aaaaaacctg tgaaaggtta
     3781 attataaaag ctgcatcttt gagagacgag tttaaaggtg aactttatgt gattcacgtt
     3841 acaaaaaatg gtcttaactt tttggataac ccaaaagaag gagaggctct gcaatatcta
     3901 tttgaaatat caaagtctgt aggtgcaaat ctgtcagtgc taaaatccga caatattata
     3961 aaatgtattg cagatttcgc caaagataat aacatcaatt atattatttt gggagaatcc
     4021 ccaaaagata cggatgataa tattttccat aaaaagctgc ggaatctatt gcataatata
     4081 gaactggaag taataccata atgcctttgt atacgcattt ttaacattaa tgttatcatt
     4141 ttgcaaaata attataaaaa tcagaatatt ttcattaaaa agcttgtttt tttaatgaaa
     4201 atattgactt aatgcggctt tcattataaa ataatgtcga atccaaatat aggaggttat
     4261 gtagatgaat aaaacagaat tagttaattc tatatcagcg aaaacagggt taagcaagaa
     4321 gaatagtgaa gcagctttaa atgcatttgt atcatctgtt gaagagtctc ttcaaaatgg
     4381 agaaaaagtt gtattggtag gttttggtac ctttgaagtt agagaaagag cagaaagaaa
     4441 aggcagaaat cctcaaacca aagaagaaat cgttatacca gcttccaagg ctccagtatt
     4501 taaagcaggc aaaggtctta aagatttagt taaataatat attgtgtaca tattagtaca
     4561 tatttatttt atttgctgtg aaagccctga tgtaatattc agggctcttt atcttttaaa
     4621 aacatttctg cagcttttca ataatctctt ttactattgc ttttacatcc ttacctgaac
     4681 aatcaattac aatatccgaa tacctctcat ataaaggcac tctttcatga tacatgtcaa
     4741 taaaactttg ctcttcattt ttagcaaacc ttctgccctg tttaattcta gaattaacac
     4801 tattaaaatc cagttgaagg tatattacta ctccatcctt ttttaaatgt accattgctg
     4861 cgtcaccgta gattacactt ccacctgttg caattatgct gttcacggtg tcaagctgca
     4921 atatgacttc ttcctgaatc tttaagaact catgatgccc gtaaagtgaa acaatatctc
     4981 ttaaatcctt cttctgtgac tccagtatca ggttatccgt atcaatgtaa ttcatattca
     5041 gaatatcagc aaggttactc cctattgtgc tttttccgga gcttggcata cctattaaaa
     5101 ctatgttgct tttagcatct ttgtaaatat aatcaccccc aatttaatgc cctatacact
     5161 ataataaatg caattactga aatgaagtct tcatggtttt atccttttgc atttattgaa
     5221 acggtgtgaa gcaatccgat tagcggattg cacttatgtt ataataaatg cgcgatgttt
     5281 tagcaaaagc accagcattt tgattaataa aaaaggaagg tgaaaaccta aacaatgaaa
     5341 catataaaaa taataaatgc cgctacttta aaaaataccg taaaaaacgg aggttgtggc
     5401 gaatgtcaga catcctgcca gtcagcatgt aaaacatcat gtactgtagg caatcagaag
     5461 tgtgaaaaga attaacctta taccttattg catgaaaaaa aagttttcac aatcaaatgt
     5521 tttgattgtg ccgcagaaag tcgtttcatg atttctggat aataaccaca ttatgattgt
     5581 tccccagaaa gccgttttac ggcttttcac tgcaggatta tctg
//
LOCUS       DAOSDD010000265         5603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5175_length_5603_cov_3.938176, whole genome shotgun sequence.
ACCESSION   DAOSDD010000265 DAOSDD010000000
VERSION     DAOSDD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_5603_cov_3.938176"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1340
                     /gene="tig"
                     /locus_tag="PK733_17045"
     CDS             54..1340
                     /gene="tig"
                     /locus_tag="PK733_17045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOJ12276.1"
                     /translation="MKSKVEKVEKNVVKFEIEVDADKFDEGMEKSFKKNAGKFNIPGF
                     RKGKAPRKIIERYYGEQVLYEDAINFICPETYDQAVKEHNINPVDRPDIDIVQIGNGQ
                     DLIFTAKVTVKPEVELGEYKGIEAEKKEVIVTDDDVEKELKESAEKNSRLVNVEDRAV
                     QEGDTAVIDFEGFVDGEAFEGGEGKNYFLTIGSGQFIPGFEEQLIGVEKGNETEVNVV
                     FPEDYSHEKLAGKAALFKVTVNEIKVKEIPAIDDEFAQDVSEFSTLEEYKADLRKKLF
                     EKEEHTIKHELEDSVIKKVVENANVDIPDVMVNERINYLLSDIDMRLRYQGMNLDNYL
                     KAVGEDIDSFKEQFSERARNEVKTQLVLEKIKDVEGIKAEDSEFDEEIRKFAENDQKN
                     EEEFKKQLTEDDIEYIRNNITVAKTINFLVNNAKLV"
     gene            1490..2074
                     /gene="clpP"
                     /locus_tag="PK733_17050"
     CDS             1490..2074
                     /gene="clpP"
                     /locus_tag="PK733_17050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOJ12277.1"
                     /translation="MSLVPIVVEQTNRGERSYDIYSRLLKDRIIVLSDEVNDITASLV
                     VAQLLFLEAEDPDKDIQLYINSPGGSITSGFAIYDTMQYVKPDVQTICIGMAASMGAF
                     LLAAGAKGKRFALPNSEIMIHQPLGGVRGQATDIKIHTEWLMKIKNKINKILSDRTGQ
                     PLDKIERDVERDFFMSAEEAKAYGLIDQVMERRK"
     gene            2088..3383
                     /gene="clpX"
                     /locus_tag="PK733_17055"
     CDS             2088..3383
                     /gene="clpX"
                     /locus_tag="PK733_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOJ12278.1"
                     /translation="MARYDEKKQVKCSFCGKSQEQVKRLVAGPGVYICNECIELCSEI
                     IDEEFEDTRVNPEYDRVPKPREIKDFLDQYVIGQEKAKKTLSVAVYNHYKRVYTDVRP
                     GDIELQKSNIVMLGPTGSGKTLLAQTLAKMLNVPFAIADATSLTEAGYVGEDVENILL
                     KLIQAADYDIERAEKGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFLQIDTTNILFICGGAFEGVDKIIQDRIGKKTIGFGAKIESSKEK
                     DIGEILNYILPQDLLKYGLIPEFVGRVPIIVTLHSLDKDALIKILTEPKNALVKQYQK
                     LFEMDDTLLEINEEALECIAEKALERKTGARGLRAILEEIMLDVMFDIPSETNIEKCI
                     ITKETVENNAQPEIIINENKKALKKAGSKKPRIKRESVS"
     gene            3633..5378
                     /gene="lonB"
                     /locus_tag="PK733_17060"
     CDS             3633..5378
                     /gene="lonB"
                     /locus_tag="PK733_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOJ12279.1"
                     /translation="MFTNTFFIIQFIFSIIIGLYFLNLLKSQQVSKSAIEKESKKEIE
                     KLRRLREIKLTEPLSEKTRPSTLDDIIGQEQGLKALRAALCGPNPQHVIIYGPPGVGK
                     TAAARVVLEEAKQITISPFKGDAKFVEIDATTLRFDERGIADPLIGSVHDPIYQGAGA
                     YGVAGIPQPKPGAVTKAHGGILFIDEIGELHSIQMNKLLKVLEDRKVYLESSYYSSED
                     SNIPSYIHEIFQKGLPADFRLVGATTRTAEDIPPALRSRCAEVFFRPLYPGEIEIIAK
                     NAALKGGFRIEPGVGELISKYAQNGRDAVNIIQMAGGISLMENRSIILVKDIEWVIEF
                     GHYNPRIDKKVCAAEQIGCINGLAVFGNSTGMVIDIEVSAIKAKSGKGTIKITGIVEE
                     EEMEGRGHKLKMSSSAKSSVENALTVLKRFLDVECKEFDIHMNFPGGIPIDGPSAGVA
                     IAVALYSAIKNFPVNGDIAMTGELSIRGKVKPVGGVVAKVEAAKLAGIKKVLIPRENW
                     QELYRNIGIDVIPVDDISEVLDIIFIKKDDRKVGWNASGAVLGAASAKGTSVAGTVLG
                     AAGDALRAQQERFRM"
BASE COUNT         2007 a          761 c         1342 g         1493 t
ORIGIN      
        1 aaataaacaa ataagtatgg ataaaacaat taaataacgg gatgacaagt tacatgaaat
       61 caaaagtaga aaaagtagaa aaaaacgttg taaagtttga aattgaagtg gatgcagata
      121 aatttgatga aggaatggag aaatccttta aaaagaatgc aggaaagttt aatatacctg
      181 gttttagaaa aggtaaagca ccgcgtaaaa ttatagagag gtattatggg gaacaggttt
      241 tatatgaaga tgcaataaat tttatttgcc ccgaaacata tgatcaggct gttaaggagc
      301 ataatataaa tcctgttgat aggcctgaca ttgatatagt acagattgga aatgggcagg
      361 atttgatttt tactgctaaa gttacagtaa agccggaggt tgagcttgga gaatataaag
      421 gtatagaagc agagaaaaaa gaagttatcg ttaccgatga tgatgttgaa aaagaactta
      481 aagaatcagc agaaaaaaac tccagattgg ttaatgttga ggacagggct gttcaggaag
      541 gggatactgc agttatagat ttcgaaggct ttgttgatgg agaggctttt gaaggtggtg
      601 aagggaaaaa ttattttctt acaataggat cggggcaatt catacctgga ttcgaggagc
      661 agcttatagg agtagaaaaa ggtaatgaaa cagaagtaaa tgtggttttc cctgaagact
      721 atagtcatga gaaattggct ggaaaggcag cattatttaa agtaacagtc aatgaaatta
      781 aagttaagga aatacctgct attgatgatg aatttgccca ggatgtaagt gaattttcta
      841 ctcttgagga atataaagca gatttgagga agaagctttt tgaaaaagaa gaacatacaa
      901 tcaaacatga gttggaagat agtgttataa aaaaggtagt agaaaatgct aatgttgaca
      961 ttcctgatgt aatggtaaat gaaaggatta attatttatt gtcagatatc gacatgaggc
     1021 tgcgttatca aggaatgaat ctggataact atttaaaagc agtaggtgaa gatattgaca
     1081 gctttaagga gcagttttct gaaagagcac gtaatgaagt gaagactcag ttggtattgg
     1141 aaaagataaa agatgttgaa ggtataaagg ctgaggatag tgagtttgat gaagaaataa
     1201 gaaaatttgc tgaaaatgac caaaaaaatg aagaagaatt taagaaacaa ttgacagaag
     1261 atgatataga atatataagg aacaatataa cagttgcaaa aactattaat tttcttgtta
     1321 acaatgcaaa gctggtttga ctttggcaaa tttgtgtggc ataggattaa gcacagatgt
     1381 gttgcacagg attaagcaca ggtgtatggt gcaaaattta gaagataact aagagtttat
     1441 ttagaaatta atagggaaag aatatacaag taaaaaggag gtattgaaca tgagtcttgt
     1501 tccaattgtt gttgaacaaa caaatcgcgg tgagaggtca tatgatattt attcaaggct
     1561 tcttaaggac agaataattg tgttaagcga cgaggtaaat gatataactg caagtttggt
     1621 ggtggcgcag ctgcttttct tggaagcgga agatcctgat aaagatatcc agctatatat
     1681 aaatagtccc ggaggatcta ttacatcagg tttcgctata tatgatacaa tgcaatatgt
     1741 taaaccggat gtacagacta tttgtattgg tatggccgct agtatgggag cttttttgct
     1801 tgctgcaggt gcaaaaggta aaagatttgc actgcctaat agtgaaatta tgattcatca
     1861 gcccctcgga ggagtacggg gtcaggctac tgatataaag attcatacag aatggttaat
     1921 gaaaataaag aataaaatta ataaaatact gagtgacaga accggtcaac ctttggataa
     1981 gattgaacgg gatgtggaga gagacttttt catgtcggca gaagaggcaa aagcttatgg
     2041 actgatcgac caggttatgg aaagaaggaa gtgaatgggg tgaagaaatg gctagatatg
     2101 atgaaaagaa acaggtaaag tgttccttct gcggcaagtc tcaggagcag gttaagaggc
     2161 ttgtggcagg accgggagtt tatatttgta atgaatgcat agaattatgc tctgaaataa
     2221 tagatgaaga gttcgaagac acaagggtta accctgaata tgatcgtgta cctaagccca
     2281 gggaaataaa ggattttttg gatcaatatg ttattggcca ggaaaaagct aagaaaacac
     2341 tttctgtagc ggtatacaat cattataaaa gagtctacac cgatgtcaga ccaggtgata
     2401 tagaacttca gaagagtaat attgtaatgc ttggacctac tggttcaggt aagactttgc
     2461 ttgctcagac tcttgccaag atgttaaatg ttccctttgc gattgcagat gcgacatcat
     2521 taacagaagc gggctatgta ggagaggatg ttgaaaatat tcttctaaag cttattcaag
     2581 cagcagatta cgatattgaa agagctgaaa agggaataat atatatcgac gaaatagata
     2641 aaattgccag gaaatccgat aatccatcta taacaaggga tgtaagcgga gaaggtgttc
     2701 agcaggccct gcttaaaata cttgagggaa cagtggcttc cgttccacca cagggaggca
     2761 gaaaacatcc gcatcaggag tttttacaga tagatactac caacattctt tttatatgcg
     2821 gtggtgcatt tgaaggagtt gacaaaatca tccaggatag aattgggaag aaaacaattg
     2881 gatttggcgc aaaaatagaa agctcaaaag agaaggatat aggggagata ttaaactata
     2941 tactgcccca ggatttgctc aaatacggat taatacctga atttgttgga agagttccca
     3001 ttattgttac attgcattcc cttgataagg acgcactaat aaaaatatta acggagccta
     3061 aaaatgctct ggtcaaacag tatcaaaaat tgtttgaaat ggatgacaca ttacttgaaa
     3121 ttaacgaaga agccctggag tgtatagcag aaaaagctct ggaaagaaaa accggtgcaa
     3181 ggggactcag ggcaatactt gaggagatta tgcttgatgt aatgtttgac ataccttcgg
     3241 aaactaatat tgaaaaatgc ataatcacaa aagaaacggt tgagaacaat gcacaacccg
     3301 aaataataat aaatgaaaat aaaaaggctc ttaaaaaggc gggttcaaaa aagccccgaa
     3361 ttaaaagaga gtcggtttca taaggaagca aatcaagatt aagttttgac aaataaagag
     3421 aaagttttgt aagtaaaaaa gaggttgcca aattggcaat ctctttttta gagtaaaatg
     3481 caaaatgatt cttcattctt ttacgtatta tatataggat aaaattatct tattagtaaa
     3541 tactatcatt agaaaaaaca aaattagaaa atgtataaaa tatattacac atttaaaaga
     3601 tatctgtggg ttttactcaa ggaggattaa gcatgtttac aaacactttc tttattatac
     3661 agtttatttt ttcaattatc ataggattgt attttctaaa tctcctgaag tcgcagcagg
     3721 ttagcaaatc tgctatagag aaggaatcaa agaaggaaat tgaaaaactg agaaggttaa
     3781 gagaaatcaa actgactgaa cctctatcag aaaagacaag gccatctacc ctggatgata
     3841 taataggaca ggagcagggc cttaaagcgt taagagcagc tttgtgcggg ccaaatccac
     3901 agcatgtcat aatatatggt cctccgggtg ttgggaaaac tgcagcggca agggttgtac
     3961 tggaagaggc aaaacaaata accatatctc catttaaagg ggatgctaaa tttgttgaga
     4021 tagatgcaac tacattaaga tttgatgaaa gaggtattgc agacccgctt ataggatcgg
     4081 ttcatgatcc tatttaccag ggagcagggg catatggagt tgcaggtata cctcagccaa
     4141 aaccgggagc tgtaacaaaa gctcatggcg ggattttatt cattgatgaa attggcgaac
     4201 ttcattccat acagatgaac aaactcctaa aagttttaga agataggaaa gtgtatctcg
     4261 aaagctctta ttatagttca gaggattcta acatcccatc atatatacat gagatatttc
     4321 agaaagggct gccggctgat ttcagactag ttggggctac taccagaact gcagaagata
     4381 ttcctcctgc actgagatcc agatgcgcgg aagtattttt cagaccttta tatcctggcg
     4441 aaatagaaat aatagctaaa aatgccgctc ttaagggtgg attcagaatt gagcctggtg
     4501 ttggtgagct tatatccaaa tatgctcaga atggaagaga tgcagtgaat ataatacaga
     4561 tggcaggtgg aatttctctg atggaaaaca gaagtattat attggtaaag gatatagaat
     4621 gggtaataga gtttggacat tataatccca ggatagataa aaaagtatgt gctgcagaac
     4681 aaataggatg tataaatgga ctggcggtgt ttggcaactc taccggcatg gttatagata
     4741 ttgaggtttc ggcgataaag gccaaaagcg gtaaaggcac aataaagata actggtattg
     4801 tagaagagga agagatggaa ggacggggac acaagctaaa gatgtcaagc tcagctaagt
     4861 cttcagtaga aaatgcatta acagttttaa agaggttttt agatgttgaa tgtaaggaat
     4921 ttgatattca tatgaatttc ccgggaggaa tacctattga tgggccttca gcaggcgtgg
     4981 ccatagcagt agcattatat tctgctatta agaatttccc agttaatgga gacattgcta
     5041 tgacaggtga gctttctata agagggaagg taaaacctgt tggtggagta gtggcaaagg
     5101 ttgaagcagc taaattggca gggataaaga aggtgcttat tccaagagaa aattggcagg
     5161 agctatatag aaatataggc attgatgtta ttcctgttga cgatataagt gaagttttag
     5221 atattatttt cataaaaaaa gatgatagga aagtcggatg gaatgcgtca ggggcagttc
     5281 tcggcgcagc aagtgcaaaa gggacaagtg tagcaggtac agttctcggt gcagcaggcg
     5341 atgcgctacg agcccagcag gagcggttcc ggatgtagca gtggcggtgc caaatacccg
     5401 gtaggaaagg ttcagggtga aggtttcggg tgcagtggga gcgcggggtg cagcggggat
     5461 ggttcttaca aaccgacgat ggggttgtcc ccaggtgaaa aatagagagg aaatgcgccc
     5521 cccaaaaatt ttttgttatt gcagaatgct cagatactgt attataatgt taatattaca
     5581 tatcatcgct atgttgcaat cat
//
LOCUS       DAOSDD010000266         5495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5305_length_5495_cov_3.510294, whole genome shotgun sequence.
ACCESSION   DAOSDD010000266 DAOSDD010000000
VERSION     DAOSDD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_5495_cov_3.510294"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..1783
                     /gene="argH"
                     /locus_tag="PK733_17065"
     CDS             353..1783
                     /gene="argH"
                     /locus_tag="PK733_17065"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOJ12280.1"
                     /translation="MKLWGGRFTKATDKNVNDFNSSIEFDCRMYRQDILGSIAHVRML
                     GKCSIISKEDATVIENTLHDILKDIDDGIIELDKNAEDIHTSIESILISRIGDIGKKL
                     HTGRSRNDQVVLDIRMYLKDEIKEIKCLLISLESTLLCIAEEHLDTILPGYTHLQRAQ
                     PVTLAHHIMAYFQMFKRDIERLADCFKRVDVMPLGSGALAGTTYPLDRQMTARELGFS
                     TITENSLDSVSDRDFIIELLSCFSIIMMHLSRFSEELILWSSHEFRFIEIDDAFSTGS
                     SIMPQKKNPDVAELVRGKTGRVYGSLVAMLTIMKSLPLAYNKDMQEDKEAVFDAIDTV
                     KMCLPVFTKLAATMKVHGRNMYNAAKGGFTNATDVADYLVKKGMPFRDAHEITGKIVL
                     HCIDTNKTIDQLTINEFKSFSPLIEENIYSEITLEKCISARNLPGGPSRVSVEASITN
                     ARSFLEYCNCVAANHQQVDYMKKPGC"
     gene            complement(1830..2141)
                     /locus_tag="PK733_17070"
     CDS             complement(1830..2141)
                     /locus_tag="PK733_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12281.1"
                     /translation="MANRKTKKVQTDADVKKKAVKLVITHLKKKVAQEYMGKEHIDNW
                     ISEMDEVLSKPEFDIVECFEMRKKLNDVIERTLDEEMRFKIRDSWYSLGRALDKKAKH
                     R"
     gene            complement(2446..3828)
                     /locus_tag="PK733_17075"
     CDS             complement(2446..3828)
                     /locus_tag="PK733_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ12282.1"
                     /translation="MTKIRKTAKISKAAKMAKIKRIISMVLVIIFLASSIAWIGYTCV
                     NKGTKGKKENKMPDGNKGYNEGIDNKYNEDNEDNEDNEGSVDISDGLIGKYNTGNDNG
                     SGECSEEGEEGNGKSEEGRKGKDKSESKNNGKNVGSGQHGEIATELSQPEIDFEKVKP
                     NEAGKIMVVMFHNFVETFTPTKYDKGEYTITFKAFEELLHNLYDKGYRLINLKDYIED
                     NISVPAGCIPVIFTFDDGTQGQFNLVKENGELKANKHSAVGIMEEFCSYYPDFGLKGT
                     FFVNLGLSTFSGEGTLSERLEYLIGKGFEIGNHTLNHISLTDVRSSDKLQQEIGGNQK
                     KMYELVPGYTMYSFSLPYGAPSNELMEYVVRGEYEGVKYENLGILEVGADPALSPVNK
                     KLNLLSIHRVRSPGINPVKFDLNWWLENLLKEDEYMSDGNPDTITIPESKADSIDTAN
                     LKGKKLIVYD"
     gene            complement(4015..>5495)
                     /locus_tag="PK733_17080"
     CDS             complement(4015..>5495)
                     /locus_tag="PK733_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12283.1"
                     /translation="DIRKETFEHIQNLSFRFFDGRPGGKIISRITNDVQAISDFINTG
                     VVSFISEIISILGIIAIIFVINYKLAAITITVVPVFILLLVCLKSASEKAWSETRKTM
                     ANINANMNETLQGMRVIQAFSRQEYNIRKFEEINTKNYRAHIHATVIQLLSWPLVEFV
                     GMAGTCAVVWLGAVMVTRDEVSVGTVIAFVNYIWKLWSPLSALSKVYNQALSAMASSE
                     RIFHILDTMPEIVDSPDALDLEDIKGTVEFMNVTFGYNPEERMVLHDISFRAEPGEVV
                     ALVGPTGAGKTSIINLLMRFYDPIEGSILIDGYNIRDIRLSSMRSKISMVLQDSFIFS
                     GSIRENILYGKLDATEDEIIDVAKATKVLNFVEKFKEGFDTDVEERGAKLSSGQRQLL
                     AFARALIADPRILILDEATSSVDTETERYIQEALKTLFKDRTSIVIAHRLSTIEHADK
                     IIVIDDGRIIEQGIHEELLDNKGMYYQLYQKQFQEYDEDAVG"
BASE COUNT         1650 a         1096 c          889 g         1860 t
ORIGIN      
        1 atccactttc gggaaagatg aagtttacaa tcaagaagat gctgaaggat tcattaatct
       61 ttttgggtta ccgttgaaag taagagcatt aatgatgaag aagatggagg aacacaaata
      121 attaaagtga atcactaaaa aagacatatt aattgataga aacctatagt tttacacttt
      181 taacttaaag ttttgatatc agctacttct atgttgaaac acaaaagctt aaaattggct
      241 tttctgataa aaaatgcaaa gctacagtag ggactaaaag ctatgaatta cgaattatag
      301 attatgaatt atgaattaca ctaaaatata gcagagcgaa ggtgataatt ttatgaagct
      361 ttggggtgga agatttacaa aagcgacaga caaaaatgta aatgatttca attcatctat
      421 agaatttgat tgcaggatgt acagacaaga tattcttgga agcatagccc atgtaagaat
      481 gctgggtaag tgtagtataa tatcaaaaga ggatgcaact gttattgaaa atactttgca
      541 tgatattctg aaagatattg acgatggcat aatcgaactg gataaaaatg cagaagatat
      601 ccatacaagt atcgaaagca ttttaatatc aaggattggc gatataggaa agaagcttca
      661 tacgggaaga agccggaatg accaggttgt tcttgatata aggatgtatc ttaaagatga
      721 aataaaggaa ataaagtgtt tgctaatttc gcttgaatca actcttttgt gtattgcaga
      781 agaacactta gatacgattc ttcccggtta tacgcaccta caaagggctc aacctgtaac
      841 tttagcacat catataatgg catatttcca gatgttcaaa agagacattg aaagacttgc
      901 cgattgcttt aaaagagttg atgtaatgcc tcttggttca ggtgcattag caggtacaac
      961 atacccgctt gacaggcaga tgacagcaag agaattgggg ttttcaacta ttacggaaaa
     1021 tagccttgat agtgttagtg acagggattt tattattgaa ttgctctcat gtttttcaat
     1081 tattatgatg cacttaagca gattcagtga agaattaata ctgtggtcat cgcacgaatt
     1141 tcgatttatt gaaattgatg atgccttcag taccggaagc agtataatgc ctcaaaagaa
     1201 aaatcctgat gtagctgaat tagtcagagg gaaaaccggt agagtttatg gctcgcttgt
     1261 ggcaatgctt acaataatga aatctcttcc tctggcatat aataaggata tgcaggaaga
     1321 taaagaagca gttttcgacg caattgacac agttaaaatg tgcttgccgg tatttactaa
     1381 attggcagcc acaatgaaag tgcacgggag aaatatgtat aatgcagcaa aaggcggatt
     1441 tacaaatgca actgatgttg ctgattactt agtaaaaaag ggtatgcctt ttagggatgc
     1501 tcacgaaatt acaggtaaaa ttgttctcca ttgcatagat accaataaaa ccatagatca
     1561 attaacaata aacgaattta agtctttttc accattaata gaggaaaata tttatagtga
     1621 aattacttta gaaaagtgta tatctgccag aaatctgcct ggaggacctt ccagggtaag
     1681 tgtggaagct tcaatcacaa atgccaggag ctttttggaa tattgtaatt gtgtggcagc
     1741 caaccaccag caggtagatt acatgaaaaa acccggttgt tagccgggtt ttttcatgta
     1801 atctgttttt taaacattgc tagcttatat tacctatgct ttgccttttt atccaatgcc
     1861 ctgcccaaac tataccacga atctcttatt ttgaatctca tttcttcatc aagtgtgcgt
     1921 tcaattacat cattaagctt tttcctcatt tcaaaacact ctactatatc aaattccggt
     1981 ttactcaata cttcatccat ttctgaaatc cagttatcta tatgctcttt acccatatat
     2041 tcttgtgcta cctttttctt cagatgagta attacaagtt ttacagcttt tttcttaacg
     2101 tcagcatccg tttggacttt tttagttttt ctatttgcca tttcaaacac tcctcaacca
     2161 ataatacttc taactaatat tcattatata ataaatgtaa tcaaattgca aactttatac
     2221 tctcttcccg tcgttagtca atatatactt atatatgcat tcatgtaaaa tgcaggaaat
     2281 cgcttaactt aatgtatata cagcactaaa ctaataatat ttatatattt ttgaaatgca
     2341 tgtggagtaa gagtttatga tttgttctcc atgtaatttc cagttattat aattatagct
     2401 attttccagc ttcattcacc ttttatacta aaacctgatt ctttattaat catacacaat
     2461 taatttcttg cctttgagat tagcagtatc gatgctatcg gccttgctct ctggaattgt
     2521 aattgtatca ggattaccgt cgctcatata ttcatcttct tttaaaaggt tttccagcca
     2581 ccagtttaag tcgaatttta ccgggttaat tcccggcgac cttactctat gtatagaaag
     2641 cagatttagt tttttattca ccggtgataa agcaggatcc gctcctactt ccagtattcc
     2701 caggttctca tatttaacac cttcgtactc gcctcttact acatattcca taagctcatt
     2761 tgaaggagcg ccatacggaa gtgaaaaaga atacatcgta tagccgggca ccagttcata
     2821 catctttttt tgattacccc ctatttcctg ctggagttta tcactgcttc taacatctgt
     2881 taaacttata tggtttaggg tatgattgcc aatttcgaaa cctttaccaa tcaaatattc
     2941 gagcctttca gataaggttc cttcaccact gaatgtagat aatccaaggt tcacaaaaaa
     3001 tgtacctttc aaaccaaaat ccggataata gctgcaaaat tcctccatta tccctactgc
     3061 agaatgtttg ttggccttaa gctctccatt ctctttcacc agattaaact gcccttgtgt
     3121 accatcatca aaagtaaata tcacaggtat acatccggca ggtactgata tattatcttc
     3181 aatgtaatcc ttgaggttga ttaaacggta gcctttatca tataagttat ggagaagctc
     3241 ctcaaatgct ttgaatgtaa tggtgtactc tcctttatca tactttgtgg gagtaaaagt
     3301 ttcaacgaaa ttgtgaaaca taactaccat tatttttcct gcctcattcg gttttacttt
     3361 ttcaaagtct atttcaggct gcgaaagctc tgtagctatt tcaccatgtt gcccgcttcc
     3421 cacatttttc ccgttatttt tactttcact tttatctttg ccctttctgc cttcttcact
     3481 tttaccatta ccttcttctc cttcttcgct gcactctcca cttccgttgt cattccctgt
     3541 gttatacttt ccaatcaggc catcagaaat atctacgcta ccttcattgt cttcattgtc
     3601 ttcattgtct tcattatatt tattatctat gccctcattg taccctttat tcccatcagg
     3661 cattttattt tctttcttcc ctttagtccc tttgtttaca caagtataac caatccatgc
     3721 tatggaactt gctaagaata taatcaccaa taccattgat attattctct ttatctttgc
     3781 catttttgct gcttttgata tctttgcagt tttccttatc tttgtcattt gtacacctca
     3841 ttttttattc agtgaaagct aataccttct acaatagcat cttctaagag caggcacctg
     3901 ggagtctgct cggaaaagcg aactcgtgca agaagaagtg tcatttcctg catacaagat
     3961 acttcgagtg caagttctaa tgccatttgc tcgttccaat gcgtgcgcag taactcatcc
     4021 gactgcgtcc tcatcatatt cttgaaactg tttttgatat agctggtagt acatgccctt
     4081 attatctaaa agttcttcgt ggataccctg ttctatgatc ctaccgtcat ctatcactat
     4141 gattttatca gcatgctcta tggttgaaag cctatgggca ataactatag aagttctgtc
     4201 tttaaacagg gtcttaagtg cttcctggat atatctttca gtttctgtat ctacacttga
     4261 tgtagcttca tcaagtataa gtattcttgg atcagcaatt agcgctctgg caaacgcaag
     4321 cagttgcctt tgaccggaag aaagcttagc ccccctttct tcaacatcag tatcaaatcc
     4381 ttctttgaat ttttctacaa aatttaatac ctttgttgcc tttgccacat caattatctc
     4441 gtcctccgtt gcatctaatt tgccatacaa tatattttcc ctgatactcc cggaaaatat
     4501 gaaagaatct tgaagtacca tgcttatctt gcttctcata gatgatagcc ttatatccct
     4561 tatgttataa ccatctatca gtatactacc ttctattggg tcgtaaaatc tcataagtag
     4621 attaataatg gaagtttttc ctgcacctgt cggacccact aaagctacta cttcaccagg
     4681 ttcagccctg aaggatatat catggagcac catcctttct tcaggattat atccaaaagt
     4741 aacattcata aattctactg ttccctttat atcttctaag tcaagtgcat cgggagaatc
     4801 cactatttca ggcatggtat ccagtatgtg gaatattctt tcagatgaag ccatagctga
     4861 aagagcctga ttatagactt tgctcaaagc gcttaaaggt gaccaaagct tccatatata
     4921 atttacaaaa gctattacag tacctacact aacttcatcc cttgtcacca ttaccgctcc
     4981 aagccagacc acagcgcatg taccagccat gcctacaaac tctaccaacg gccaggataa
     5041 aagctgtatt accgttgcat gtatatgagc cctataattt ttcgtattaa tttcttcaaa
     5101 tttgcgtata ttgtactcct gtcttgaaaa ggcctgtata accctcatac cttgaagagt
     5161 ctcgttcata tttgcattaa tgtttgccat ggttttcctt gtttcggacc atgccttttc
     5221 actggcactc ttaagacaga caagcagtag tataaaaact ggaactacgg taatcgtaat
     5281 agcagctagt ttgtaattta tgacaaatat tattgctata attcccaata ttgatattat
     5341 ctcgcttata aacgaaacaa cacctgtatt tataaagtcg cttattgcct ggacatcatt
     5401 tgttatcctt gaaataatct tacctcccgg ccttccatca aaaaacctaa aggataggtt
     5461 ctggatatgc tcaaatgttt ctttcctgat atcat
//
LOCUS       DAOSDD010000267         5486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5312_length_5486_cov_4.905174, whole genome shotgun sequence.
ACCESSION   DAOSDD010000267 DAOSDD010000000
VERSION     DAOSDD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_5486_cov_4.905174"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..353
                     /locus_tag="PK733_17085"
     tRNA            278..353
                     /locus_tag="PK733_17085"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:311..313,aa:Val,seq:tac)
     gene            499..1611
                     /locus_tag="PK733_17090"
     CDS             499..1611
                     /locus_tag="PK733_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOJ12284.1"
                     /translation="MFYPLKFVPVYKDYIWGGRNLEKLGKKLPEGKVAESWEVSCHPD
                     GESIISNGLFKGKSLTDYVNEFNYKVIGTGLSWKPGDRFPLLIKLIDANDRLSVQVHP
                     DDEYASLHEEDKNGKNEMWYVISAKPGAKLVYGLKTGTTREKLENAVRNNSIEEHLNY
                     IQVAAGDALNIPAGLVHAIGSGILLAEVQQNSNNTYRVYDYERVDINGNKRPLHIEKA
                     LEVISYDEEASCNDDVSNINTFNNKKMVENYQPENSITHLVSSKYFAVDLIDISSEGE
                     IEEIADGSRFYIYLFIKGDGVISYSNNGDSSHKNNGDSSRTGSQKCTQSTGCFFNKID
                     VKMGESVFIPASMGNYKISGNIKCIKAYIPLCHMKL"
     gene            1745..4039
                     /gene="glgB"
                     /locus_tag="PK733_17095"
     CDS             1745..4039
                     /gene="glgB"
                     /locus_tag="PK733_17095"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOJ12285.1"
                     /translation="MKTTVNINDVLMIANAEHYDPFNVLGMHFADLGADTGVDICSDY
                     PNTACDTGCDTGKILVVRALLPNVESASVVDLECENEQGISACYEMCKIHESGFFEAV
                     ISDRKSFFKYNIQIKDYCGNTYRIFDPYYFPPVLTDFDLYLFNGGNHHKIYEKLGAHT
                     CTINEVKGTLFAVWAPCAKRVSVVGNFNQWDGRRHQMRSRGPSGVWEIFIPEIDKGEI
                     YKYEIKTASNELYLKSDPYAFCSELRPGTASIVYDLSNYCWNDSQWMKQRDSGNFFDK
                     PVSIYEVHLGSWARVTEEEERFLTYTELADKLVNYVKEMGYTHIELLPIAEHPFDGSW
                     GYQITGYYSLTSRYGKPEDFMYFIDRCHQEGIGVIVDWVPAHFPKDGHGLARFDGSAL
                     YEHYDQKQGEHPDWGTHIFNYGRHEVRNFLVSNALFWFEKYHIDGLRVDAVASMLYLD
                     YGKKDGEWIPNKWGGKENVAAIEFLRQLNTTVFTYYPGVMMIAEESTSWSLVSKPPYV
                     GGLGFSFKWNMGWMNDYLRYISMDPIYRKYNHNNLTFSIMYAFSENFILVLSHDEVVH
                     GKCSMINKMPGDYNQKFAGLRASYGYMYGHPGKKLLFMGGEFGQFIEWDYKRSLDWHL
                     LDYDMHKKLQRYVKDLNALYKREKAFYEVDFNYKGFEWIDCNDSDYSVISFIRKSNDE
                     GDILIFVFNFTPVAHSDYRIGVPFSAFYKEIFNSDSEIYGGSNMGNLGGVKADEIVFH
                     NKPYSISLQIPPLSMLVLKPIKEN"
     gene            complement(4232..4306)
                     /locus_tag="PK733_17100"
     tRNA            complement(4232..4306)
                     /locus_tag="PK733_17100"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4271..4273),aa:Glu,seq:ttc)
     gene            complement(4312..4388)
                     /locus_tag="PK733_17105"
     tRNA            complement(4312..4388)
                     /locus_tag="PK733_17105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4352..4354),aa:Met,seq:cat)
     gene            complement(4624..>5486)
                     /locus_tag="PK733_17110"
     CDS             complement(4624..>5486)
                     /locus_tag="PK733_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12286.1"
                     /translation="GIACYLGSEGLDGILNVLSGELDPDDPDSMHIQKCLMASFEDRE
                     MLSGEDRKVIKELGLKFRGRNQWPLFRNHFPGFFPWFITARESRFLTNILIQAMHVAL
                     ICRDEGKEFLYHDEPLTFLTRIPEEKNGRLEWYDEYIAATPYTSQYASYHLEDEIKLK
                     RLQLLGRKKGSAWEIDTFYSPGPIKDNDRPYYPKICLVIEQTRGLILCFDMVKDIKND
                     GFKFIDLITNLIERSGHFPSRIFVQRDETYYLLKDICSQLGINIEMVNRLHILQDARY
                     EMYNFFNGFY"
BASE COUNT         1833 a          854 c         1086 g         1713 t
ORIGIN      
        1 aaaaattgat ataagcgtgt ttgttaaaca ttacttcata ttcaaaaaac gcatacgtaa
       61 aaaatgcaca catatgatat tgaaggaatg aaatattgat aaatataata ggaatatatt
      121 gataaatatg atataaatat ttagataata taagtataac ctaatatagg caagataata
      181 tgagataata ccatataatc tgtaattgtg gacaacgtta aaattgacat tgaaatatat
      241 attttatata ctatttattg ttccacaaaa ttcgtaaggg cgcttagctc agctgggaga
      301 gcacctgcct tacaagcagg gggtcatagg ttcgatccct atagcgccca ccaaaaacaa
      361 ggtgtaagat ttttaaattc ttataccttg tttttttgta tacaatatca tttaaaattt
      421 cttatttaaa tgataaaata atcatatgga gtggtattaa gaaacatatt ataataatta
      481 aatactttgg aggcgtaaat gttttatcct ttaaagtttg tacctgttta taaagattat
      541 atatggggag gtaggaatct tgagaaactg ggcaaaaaac ttcctgaagg aaaagttgct
      601 gaaagctggg aagtatcctg ccatcctgat ggagaaagta taatttcaaa cggcttgttt
      661 aaagggaaat cccttactga ttatgttaat gagtttaatt ataaagtaat agggacaggt
      721 ttatcatgga agccaggcga caggttccct ctgcttatca agctgattga tgcaaatgac
      781 aggttatctg tgcaagtgca ccctgatgat gagtatgctt ccctgcatga ggaagataag
      841 aacggtaaaa atgaaatgtg gtatgtaata tctgccaagc cgggggcaaa gcttgtatac
      901 ggcctgaaga ccggtactac ccgtgaaaag ctcgaaaatg cagtaagaaa caattctatt
      961 gaggaacacc ttaattatat ccaggttgca gcgggggatg cattgaacat acctgcaggc
     1021 cttgtacatg ctattggcag cggaatattg cttgccgaag ttcagcagaa ttccaataac
     1081 acatatagag tatatgatta tgaacgcgta gacataaatg ggaataaaag acccctgcat
     1141 attgaaaaag cattggaagt aattagttat gatgaggaag ccagttgcaa tgatgatgtg
     1201 agtaatataa atacgttcaa taacaaaaaa atggtagaaa attaccaacc tgagaattcc
     1261 atcacacatc ttgtgtccag taaatatttt gctgttgatt taattgatat atccagtgaa
     1321 ggtgaaatag aggaaattgc tgatggaagc aggttttata tatatctttt tattaaagga
     1381 gatggagtta taagttattc taataatggt gacagttctc ataaaaataa tggggacagc
     1441 tctcgaactg gttctcaaaa gtgtactcaa agtaccggat gcttttttaa taaaatagat
     1501 gtaaaaatgg gggagtcagt ttttatacct gcatctatgg gaaattataa aatatccggt
     1561 aatattaagt gcataaaagc atatatacca ttatgccata tgaaattata acatttaaaa
     1621 taaggtttta aaaaagtata atatgaatat aattatatac actaactgaa tgtaataatg
     1681 cggttcttta tcattaatca ctactgcaac cgcaaaaata aaatacatag aagggaaaat
     1741 ttagatgaaa actactgtta atattaatga tgttctaatg attgcaaatg cagaacatta
     1801 tgaccctttt aatgttttag gaatgcattt tgcagatttg ggagcggata cgggagttga
     1861 tatttgctct gactatccca atacggcttg cgatactgga tgtgatactg gaaagatatt
     1921 agttgtaagg gcacttttgc ctaatgtaga gagtgcctca gtagttgatc tggaatgtga
     1981 aaatgaacaa ggtatttctg cctgctatga gatgtgtaaa attcatgaaa gtggtttttt
     2041 tgaagcggta atatccgaca gaaaaagctt tttcaaatat aatatacaaa ttaaagacta
     2101 ttgtgggaat acttatagaa tatttgaccc ttattacttt ccaccagtac ttacagattt
     2161 tgacctgtat ctctttaacg gaggaaatca ccacaaaatc tacgaaaaac ttggagcaca
     2221 cacctgcact ataaatgaag tgaaagggac tttatttgct gtttgggctc catgtgcaaa
     2281 gcgggttagt gtagtaggca atttcaatca atgggatgga agacggcatc aaatgagaag
     2341 ccgtgggcca tctggtgtat gggaaatatt cataccggaa attgataaag gcgaaatata
     2401 taaatacgaa ataaagactg ctagtaacga gctttatctt aaatccgatc catatgcttt
     2461 ttgctcggaa ttaagacctg gtacagcatc aatagtatat gatttaagta attattgctg
     2521 gaacgacagc caatggatga aacagagaga tagcggcaat ttctttgata aacccgtatc
     2581 aatctacgag gtgcatttag ggtcatgggc aagagttacg gaagaagaag aaagattttt
     2641 gacatatact gaattggccg acaaacttgt aaattatgtt aaagaaatgg gttataccca
     2701 catagaattg cttcctatag ccgagcaccc atttgatggt tcctggggat atcagataac
     2761 gggttattat tctcttacca gcaggtatgg taagccggaa gattttatgt attttattga
     2821 cagatgtcat caagaaggta taggcgtgat tgtagattgg gtaccggcac attttcccaa
     2881 ggatgggcat ggccttgcac ggtttgatgg atccgctctt tacgagcact atgatcaaaa
     2941 gcagggtgag catcctgatt ggggtactca tatattcaac tatggcagac atgaggtgag
     3001 aaatttcctt gtttcgaatg ctcttttctg gtttgaaaaa taccatatag atggtctgag
     3061 ggttgatgct gtagcatcta tgctttatct ggattatggt aaaaaagatg gagaatggat
     3121 acctaataaa tggggcggta aagaaaatgt tgctgctata gagtttttac gacagcttaa
     3181 tactacagtt ttcacatatt accctggagt catgatgatt gctgaagaat ctacttcatg
     3241 gtcccttgtc tcaaagccac catatgtagg ggggttgggg ttttccttta agtggaatat
     3301 ggggtggatg aatgattatt tgagatatat tagcatggac cctatatata ggaaatataa
     3361 ccataataat ctaacttttt caataatgta tgctttctct gaaaacttta ttcttgtgct
     3421 ttcccatgat gaagtagtac atggaaagtg ttcaatgata aataaaatgc cgggagacta
     3481 caatcaaaag tttgcaggac tcagggccag ttatggttat atgtatggac atccaggcaa
     3541 gaaactctta ttcatgggag gagaatttgg acagtttatt gaatgggact acaagcgaag
     3601 ccttgattgg catttgcttg attatgacat gcacaaaaaa ctgcagagat atgttaaaga
     3661 tttgaatgct ttgtataaaa gagaaaaagc tttttatgaa gttgacttta attataaagg
     3721 ctttgaatgg atagattgta atgactcgga ttatagcgta atttctttta ttcgtaaaag
     3781 caatgatgaa ggggatatac taatttttgt atttaatttc acgcctgtag cacattctga
     3841 ttacaggata ggagttcctt ttagtgcttt ttacaaagaa atattcaata gtgattcaga
     3901 aatttatggt ggaagcaata tgggcaatct tggcggtgtt aaggcagacg aaattgtgtt
     3961 tcacaacaag ccatattcaa taagcttgca gatacctcct ttgagtatgc ttgttttaaa
     4021 gccgattaag gaaaattaga caaaagactg ccttaaatta taaataaaaa tggtttggag
     4081 acatttttat ttataataca aaatatggta agaagtgatt tcaggtatga tattcacttc
     4141 agcaatggat tgtagagttt aagtctacat gaaaatttca tatttgttat aaaaaaacag
     4201 ttttaaatta taaataattt aaaactgttt ttggtgaccc ataggggact cgaacccctg
     4261 ttatcgccgt gaaagggcgg tgtcttaacc acttgaccaa tgggccattt atggtagcgg
     4321 cggtcggatt tgaaccaacg acactgcggg tatgaaccgc atgctctcgc caactgagct
     4381 acgccgccgc aaaaggatta attaaccctg tttcattacg taacccgtaa catgcttctc
     4441 tattttaaca gatacatatc agtaaagtca agaaaaactt tttatatttt ttatatggac
     4501 gcattttctg tatatgacaa aaaaatatgg cattattcta ttaacaaagt atatgatacg
     4561 cagtaaaaac ataccacaat acatgaaaaa gcgcatttaa gttttgcttt tcacaatagt
     4621 tattcaataa aacccattaa agaaattata catttcatat cttgcatcct gtaaaatatg
     4681 cagcctgttt accatttcta tatttatgcc taattggctg catatatcct tcaatagata
     4741 atatgtctca tctcgttgca cgaatattct agaggggaaa tgtccagaac gctcaataag
     4801 atttgtaatc aaatcaataa atttaaagcc atcattcttt atatctttta ccatatcgaa
     4861 acaaagtatt aatccccttg tttgctctat taccaggcat attttgggat aataaggccg
     4921 atcgttgtcc ttaataggtc cggggctgta aaaagtatct atttcccagg cagagccctt
     4981 cttacgtcct aatagttgta atcttttaag ttttatttca tcctctaaat ggtaagaagc
     5041 atactgggat gtataaggtg ttgctgcaat atattcgtca taccattcga gtcttccatt
     5101 cttttcttcc ggaatccggg ttaaaaaagt caggggttca tcatgatata gaaattcctt
     5161 gccttcatct ctacatataa gggcaacgtg cattgcttgt ataagaatat ttgtaaggaa
     5221 cctggattct ctggcagtaa taaaccatgg aaagaaccct ggaaaatgat ttctaaataa
     5281 aggccactga tttcttcccc tgaattttag tcccagttct ttaataacct tccgatcttc
     5341 tcccgaaagc atttccctgt cttcaaaaga tgccataaga catttctgta tatgcatact
     5401 gtcagggtcg tctgggtcca actctcctga tagcacatta agtattccat caagtccttc
     5461 agagccaagg tagcatgcaa taccaa
//
LOCUS       DAOSDD010000268         5480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5320_length_5480_cov_3.272995, whole genome shotgun sequence.
ACCESSION   DAOSDD010000268 DAOSDD010000000
VERSION     DAOSDD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5320_length_5480_cov_3.272995"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PK733_17115"
     CDS             <1..654
                     /locus_tag="PK733_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ12287.1"
                     /translation="HPPELIPEMVRLWKEEGYEVVEGVKALRGKESLINKAGANLFYR
                     VLSKFSGFDINQASDFKLLDRKVVQAWKSMNERHTFFRGMSAWVGFNRASIPFNIRNR
                     TNGTSKWSLFRLFKLAINAITSFSSFPLHIVTFMGLVFLTGSLILGIQTIYMKLKGIA
                     YSGFTTVILLLLIIGSALMISLGIIGTYIAKIYDEVKQRPRYIVAEKTESEKELFVK"
     gene            complement(922..1347)
                     /locus_tag="PK733_17120"
     CDS             complement(922..1347)
                     /locus_tag="PK733_17120"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOJ12288.1"
                     /translation="MVDSDVIQRRLTALEEYISDLEKARKDKCWEEFASDKIFRRYVE
                     RTLHMAVEACLDIANYIISYEGYREPKDNKDSFEVLKEQNIITNVLNTQLKKMAQFRN
                     IIVHDYLRIQPEIVYAILKKNIPDIINFAKRIKDNYLSF"
     gene            complement(1352..1762)
                     /locus_tag="PK733_17125"
     CDS             complement(1352..1762)
                     /locus_tag="PK733_17125"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ12289.1"
                     /translation="MDEMVIQTLAEYFSNIENTDAVYLFGSFAKNKDRKHSDIDIAVL
                     FSVEMNIVERFEKKLRIASELEDIFHRKVDIVDLESADSFFVHKIMEEKVLIYNINVE
                     RRVVFEVKKRKDFFDRQKFYNLYNECSMKRLEGM"
     gene            complement(2023..2262)
                     /locus_tag="PK733_17130"
     CDS             complement(2023..2262)
                     /locus_tag="PK733_17130"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOJ12290.1"
                     /translation="MQRFIILLLQLNIDENYAVYVDGKPCSNLVERDEILKAADPDAV
                     SIQYDDLKKYKKDELRDFFVGKSVAFGSTYCKIEL"
     gene            complement(2219..2395)
                     /locus_tag="PK733_17135"
     CDS             complement(2219..2395)
                     /locus_tag="PK733_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12291.1"
                     /translation="MAELSSKQIRDKLNSEFAGDSCKIVFWYDSNKEFVKDIKNLELV
                     NAKIYYLTPTIEYR"
     gene            complement(2486..2698)
                     /locus_tag="PK733_17140"
     CDS             complement(2486..2698)
                     /locus_tag="PK733_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12292.1"
                     /translation="MKLACSITTDVILFNIDSKSETSGRKDKDTILNVFLKVFNEKLG
                     YSTNPHVADMERQLDEEGLYDDSKER"
     gene            complement(2752..4974)
                     /locus_tag="PK733_17145"
     CDS             complement(2752..4974)
                     /locus_tag="PK733_17145"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12293.1"
                     /translation="MHPDWENEVRRLEIVKRIIGSELLERTRNSDEYRKQMRLINKEM
                     WEGAGALSGLSTLDETPTFIQDIALLKRNISEQSENSRKVKMLERQINSPYFGRFDFR
                     EDNWDTESFYIGIYGLRRADTGEILIYDWRAPVSSMFYDYEPGRALYECPSGYIEGEL
                     VLKRQYRIEEGRLILMFDSNVAIEDNILQDILAGSSNNRMKTIVSTIQREQNRAIRYE
                     GKRVIAVQGPAGSGKTSIALHRAAYLLYRYRDNLKAENISLFTPNGAFSKYISTVLPE
                     LGEDDLHCITLVELAQDVLGNLFKKYESYAEMMEWQLTRKCSDELSSRWESMHFKTSK
                     QFATVLERFAETFENSIMQFDEIRDGDIVFADKEELEELFHNSYHHMPLTKRLSRMEL
                     VVTTRIKEYEKQRRQEIREELANSTEYISNSEIKVLSRRKVTRELEKVKNDVVNMLSI
                     NIASLYCMLFEDFAVWNSCGGTLSDETRRNTVNTLKGGILYYEDQAPILYLMVLLGMI
                     DPGKEIKHVIIDEAQDYSEVAFKLFSQLYSHCTVTLLGDLNQNINPVSGIGNLRSAGE
                     LTDTKNFEYIELDKSYRSTVEIMEFASRIISSKTIPFGRSGKVPEIVTAETMKAVCNL
                     IIGYVNGIKDKKFRSIAIICRTLSGCHQVYKYLKKELSANLITNGDNEIPSGIIVIPS
                     YLTKGLEFDIVIAVVLSADEYMFNEEQLFYTVCTRALHRLEIFGVEKAGILEKIATLC
                     "
BASE COUNT         1677 a         1088 c          828 g         1887 t
ORIGIN      
        1 catccgcccg aactgattcc tgaaatggtt cgcctatgga aagaagaggg ttatgaagtt
       61 gtggaaggag ttaaggcttt acgcggaaaa gaaagcttga ttaataaggc gggggctaat
      121 ttgttttatc gtgtattaag caaattttcc ggatttgaca taaaccaggc ttcagatttc
      181 aaactgttag atagaaaagt tgttcaggca tggaaaagta tgaatgaaag acatactttt
      241 ttcagaggga tgtctgcatg ggtggggttt aacagagcaa gtattccttt taatataagg
      301 aacaggacaa acggaacatc caaatggtcc ttgtttaggc tattcaagct tgcaataaat
      361 gccataactt cattctcatc atttcccctc catatagtta cttttatggg gttggttttt
      421 ctcaccggtt cattgatact tggaattcaa accatatata tgaagctcaa aggcatagct
      481 tatagtggct tcacaacagt tattcttctg cttttaataa tcggaagcgc cctaatgata
      541 agcctgggaa ttattgggac ctacatcgcc aaaatatatg atgaagttaa acaaagaccc
      601 aggtacattg tggcagagaa aacagaaagt gaaaaagagc tgtttgtcaa gtaaacttcc
      661 agctacccca aagtgtagtg acttgtagga agtaatttcc ataagctggc tgattagcct
      721 aagtcctaac tgactacgat taataagaat atatatgtac ttaaagatgc ttctctagtc
      781 ttaaacacta tgctttaata ttaaacattt ctgatggcag gaaaagtgta ttaaagatat
      841 aaaaccttat caatcattgg cgaagagaac ctaccgcaga aatgcgagtt taaataatct
      901 gaaaggatgt atataatgta gttaaaaact caggtaatta tctttaattc gttttgcaaa
      961 attaataata tcaggtatat tctttttcag aatagcatac acaatttctg gttgtatcct
     1021 gagataatca tgtacaataa tatttcgaaa ttgggccatt ttttttaatt gagtattcaa
     1081 cacattggtg attatattct gttctttcaa aacttcaaag ctatctttat tatcttttgg
     1141 ttcacgatat ccctcatatg aaataatata attggcaata tctagacaag cttccactgc
     1201 catatgaagt gttcgctcaa cataccttct gaaaatttta tctgaggcaa attcttccca
     1261 acatttatct ttccttgctt tttctaaatc acttatgtat tcttccaatg ctgttaagcg
     1321 cctttgaatt acatccgaat ctaccataat atcacatccc ctccagcctt ttcatagaac
     1381 actcattata tagattataa aatttctgtc tgtcaaaaaa atcttttcgc tttttcactt
     1441 caaagactac tcttcgctct acatttatat tataaattaa gactttctct tccatgatct
     1501 tatgaacaaa gaatgagtct gcgctttcca aatcaactat atcaaccttc ctatgaaaaa
     1561 tatcttcaag ttctgatgca attctgagtt ttttctcgaa tcgttctact atgttcattt
     1621 caacagaaaa cagcacagct atatctatat cactatgttt tctgtctttg ttttttgcaa
     1681 atgaaccaaa taaataaact gcatctgtat tttcaatgtt gctaaagtat tcagcaagtg
     1741 tttgaataac catttcatcc atggtgctgt tctcatctcc tgttttatat agactattta
     1801 tatctattat ttgcatttaa tctataattt aattatacac taccagggtt caaatgagcc
     1861 aatactatta tatcactata ttgaaataat attaaatatt tgtacatttt atactatgtt
     1921 aacttaagca attttttgta aaaacccact aatggataag tatgagatat tggcattcca
     1981 cgaagaccta aattagaaaa ctcagcttca taagtatcag ttctatagtt caattttaca
     2041 gtatgtcgaa ccaaaagcca cggatttgcc cacaaagaaa tctcgcaatt catccttctt
     2101 atatttcttc aaatcatcat attgaatgct tacagcatca ggatctgcag cttttaatat
     2161 ttcatctctt tccaccaggt tagagcaggg tttcccgtct acataaacag cataattttc
     2221 atctatattc aattgtagga gtaagataat aaatctttgc atttaccaac tccagatttt
     2281 taatatcttt tacaaattct ttattgctgt cataccagaa aacaattttg cagctatctc
     2341 cggcaaattc ggagtttaac ttatctctta tctgttttga gcttaattca gccatatatt
     2401 atctctccta tattattttt ctatctaatc tttgctaata cattcaaatg tttacctttt
     2461 tcatcagttt gaactttatc gtaattcacc tttctttaga atcatcatac agcccttctt
     2521 catctaattg cctttccatg tcagctacat gcggatttgt gctatatccc agtttttcgt
     2581 taaatacctt taaaaataca ttaagtatag tatccttgtc ttttcttcct gacgtttcac
     2641 tttttgaatc aatattaaat aaaataacat cagtagtaat tgaacaggcc aacttcatat
     2701 cagttataac cacagggtct tcaactatta tataccatca attattatag ttcagcacaa
     2761 agtggctatc ttttctaata ttcctgcctt ttccacaccg aaaatttcta gtctgtgaag
     2821 tgctcgagta catacggtat agaaaagctg ttcctcatta aacatatatt catccgcaga
     2881 tagcactact gctatgacaa tatcaaattc cagacctttg gtcaggtagg acggtatcac
     2941 aatgatacca gaaggtattt cattatcccc atttgttatg agatttgctg ataattcctt
     3001 tttaagatat ttgtatacct gatggcaacc gcttaaggtt cgacagataa tggcaatcga
     3061 acggaatttt ttatctttta taccgttcac ataaccaata attaaattac atacagcttt
     3121 catagtctcc gctgtcacta tctccggcac ttttccgctt cttccaaatg gtatggtttt
     3181 cgaagaaatt atccgtgaag caaattccat aatctctact gttgaacggt aacttttatc
     3241 aagctcgata tattcaaaat tttttgtatc agtgagttct cccgctgatc gcaaattccc
     3301 tattccactt acagggttta tgttctggtt caaatcaccc aaaagtgtaa cagtacagtg
     3361 agaataaagc tgtgagaaca acttgaatgc aacttcagaa taatcctgtg cttcatcaat
     3421 tattacatgc ttaatttcct tgccggggtc gatcattcct agcaaaacca tcaaataaag
     3481 gataggggct tgatcctcat aataaaggat accacctttc agagtattca ccgtattcct
     3541 tcgggtttca tccgaaagag ttccaccaca ggaattccaa accgcaaagt cttcaaacaa
     3601 catacagtaa agactggcaa tattaataga tagcatgttt actacatcat ttttaacctt
     3661 ttccagttct cgtgttactt tacgacggct taacactttt atctcagaat tactgatata
     3721 ttccgtactg ttagccagtt cctcccttat ttcctgtcgt ctctgcttct catattcctt
     3781 tatacgtgtt gttactacta actccattcg tgaaagcctt ttagttaaag gcatatggtg
     3841 atagctattg tgaaaaagct cttcaagctc ctctttgtca gcaaatacaa tatctccatc
     3901 tcttatctca tcaaactgca taatactatt ctcaaaagtt tcggcaaatc tctccaatac
     3961 agtagcaaac tgtttcgatg ttttaaaatg catactctcc catcggctgc ttaattcatc
     4021 tgaacatttc cgtgtaagtt gccattccat catttccgca taagattcat attttttaaa
     4081 cagatttccg agcacatctt gtgcaagttc aaccaatgtt atgcagtgta aatcgtcttc
     4141 acccagttca ggtaatacag tcgaaatgta tttggaaaaa gctccattcg gtgtgaataa
     4201 agaaatattc tctgccttta aattgtctct atagcggtaa agtaagtatg cggctctatg
     4261 aagtgctata gaagttttgc cactgcctgc aggcccctgt actgctatta ctcgtttgcc
     4321 ttcataccgt attgcacggt tttgttccct ctggatagta cttactattg ttttcatcct
     4381 gttatttgaa cttcctgcaa gtatgtcctg taaaatatta tcctctatag caacgttact
     4441 atcaaacatc aatataagcc gaccttcttc aattctgtac tgccgtttga gaacaagctc
     4501 gccttcaata tatccagagg ggcactcata taaagctcgt cccggttcat agtcataaaa
     4561 catactgctt accggtgccc gccaatcata gatgagaatc tctcctgtat ctgctctacg
     4621 gagtccatat ataccaatat agaaactctc cgtatcccaa ttatcttccc ggaagtcgaa
     4681 ccggccaaag taaggtgaat taatctgccg ttcaagcatt ttaacttttc tgctattttc
     4741 tgattgctca ctaatattcc gttttaacaa agcaatatcc tgaataaaag ttggagtttc
     4801 atctagtgtt gacagcccgg aaagtgcacc ggctccctcc cacatttctt tattaattag
     4861 tcgcatctgt tttctatatt catcactatt ccttgttctt tcaagcaatt cactaccaat
     4921 tatccgtttt acaatttcaa gccttctaac ttcattttcc caatctggat gcatatatta
     4981 ctcctttgta tttacattat aaattttatt ttataattat ttgtaatatt ttacaacccc
     5041 ctttaataag aaggataaaa gaagaagcga attattcaag acgaactagt agaaaagttc
     5101 aagattttag caggcagata taaaatatat ggaataatag tttgcctgct gtaaaaaagg
     5161 ttgaaactgt tcatgttatt tttcaatgta atctactttg taagctgaat cttatgaagt
     5221 acaaacccca ttacacagtc agcatcaaaa atagcttttt tcatatcatt ctctgtgata
     5281 tcaatctcaa atagataccg cagctgtaca ccatacatgg attgttaagt ttagggcaag
     5341 ggaaaatttg taattcacga cacggatgcc attattgggg gtgagaggtg agagattagg
     5401 gattagagat tggaggttag agattagaga acaattcatc ctccgagata aacaacggag
     5461 gttttcttgt gccgacttta
//
LOCUS       DAOSDD010000269         5473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5329_length_5473_cov_5.574935, whole genome shotgun sequence.
ACCESSION   DAOSDD010000269 DAOSDD010000000
VERSION     DAOSDD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5329_length_5473_cov_5.574935"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..489
                     /gene="rpsF"
                     /locus_tag="PK733_17150"
     CDS             202..489
                     /gene="rpsF"
                     /locus_tag="PK733_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOJ12294.1"
                     /translation="MLKNYEALYIIDAQLEENAIKETVEKFSTLISTSAQLKSTDEWG
                     KRRLAYEINKKNEGYYVLTEFSAEPDFPQELERIFKITEGIIKYLIVNRDN"
     gene            558..1007
                     /locus_tag="PK733_17155"
     CDS             558..1007
                     /locus_tag="PK733_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ12295.1"
                     /translation="MNKAILLGRLTKDPELRYTSTNNTAVCNFTIAVNRRFSKPGEEK
                     QADFIPVVVWDKLAEFCGKYFAKGRQVAVVGRIQTRTWDDNEGKRHYVTEVVAEEAYF
                     ADSKPDNSGMPGMPRTNESASPAFEGGAASGRDDGFYQTEEDDELPF"
     gene            1073..1366
                     /gene="rpsR"
                     /locus_tag="PK733_17160"
     CDS             1073..1366
                     /gene="rpsR"
                     /locus_tag="PK733_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOJ12296.1"
                     /translation="MPTGPKRERGSGRDSYDKGDSKGGMRMRRQKKKVCVFCSDKTAN
                     IDYKDIAKLKKFISERGKILPRRISGNCAKHQRELTRAIKQARHIALLPYISE"
     gene            1714..3009
                     /gene="pheA"
                     /locus_tag="PK733_17165"
     CDS             1714..3009
                     /gene="pheA"
                     /locus_tag="PK733_17165"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOJ12297.1"
                     /translation="MIKIGYLGPKGTFSQEAVKKYINELIIEGSINYANNYREVKEVK
                     GNKSDSNSIMDNSVKEIKISEDTSGINTANNNGLNINAANINAPNINAANNNAFCINP
                     AIDNALSINKANDNEAGISKTNNTRNFDYTVNGNKYSNDKHNEDVYSDDNDNNENIKN
                     KRINVADTICTNISINAYSEQAYNNIGDMLAAIKQGYIHEAVVPIENSLEGPVNETLD
                     LMASDMDLKIKGEVIIPIRQNLLVRKGTCINDIKYILSHPQPVGQCRDYIFKNFPEAE
                     IKLVYSTAGAAEMVSKDEYGFTAAAIGSLTAADVYGLEILAKNIQDGENNFTRFVIIA
                     GEDSGKTGDDKTSLVFSTENKPGSLYRILEIFNLWDINMTKIESRPAKKQLGQYIFFV
                     DIEGHREDEDVKDALIMVKRKTSYFKLLGSYPVFKRKKM"
     gene            3044..3382
                     /locus_tag="PK733_17170"
     CDS             3044..3382
                     /locus_tag="PK733_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOJ12298.1"
                     /translation="MGFMEKDIDITRNIKIIERLKSELLTDIANLFRILVNGAREEIH
                     EAISEALSNIILICYLLGRRLGVSYNSIEMKMDGKIRLGLVESDDMEKYYGDLSELAR
                     HLNSSRAKKK"
     gene            3394..5394
                     /locus_tag="PK733_17175"
     CDS             3394..5394
                     /locus_tag="PK733_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOJ12299.1"
                     /translation="MDNRKFTHVFMPRTSSYIWIILILLLTITVLDWRVALPGYLLFF
                     VLVFYNFRANYKRQKDIIKYIENLTFNIDSASKDTLLNFPMPLVVVELDGTIIWYNQK
                     FRKIFREEELLENTISSFVDELHPYNMIDDEVNIFREIKINDNYYRVLGNFVKNQGRT
                     DVTEYILLLYFLDISDLMELRTRYNNEKPVSGIIIIDNYDELMQSTDDTARPQILAEI
                     DKKIVQWLGFTGGILKKFERDKYLFMVEYKYMKELEDRKYDILDTIKEISVGNKIPVT
                     LSIGFGINNGNFSGNMSAASASIDLALGRGGDQVVIKDGDNFRFFGGRTGELEKRTRV
                     KARVIAYALRELIDQSYQVMIMGHENPDVDALGAALGIYRIAKSREKDASIVLSQSNP
                     AIESLLSRIEKTGEYGNLFIGKNEALDRINSKTLLVIVDTYRAKYTEHPELLKYTDQI
                     VVIDHHRRGVDFIQEAALTYQEIYASSTCEMVTEMIQYVDNKLKLKAMEAEALYAGIV
                     MDTKNFTFKTGVRTFEAASFLRKQGVNTLAVKKLFQNDLSTFLNISSVVRDAEIIFEN
                     IAISVCPPNIRNGLLIAAKSADEILNLSGIKAAFVLCTANNGVAISGRSFGDINVQVI
                     LEKLGGGGHQTVAGAQIENISLEEGKEKLKYVIINTINEIKP"
BASE COUNT         1938 a          790 c         1212 g         1533 t
ORIGIN      
        1 atgccatatt ttggactaaa tgtaatttcc aacccaaggg taaaaaatag tatataattt
       61 tttactcctc agagctgact tcattacagg aatgcttgta ttatttaaaa caatatggta
      121 gaatgtcttg tcaatacaat gcatccttgc tctgcactca tgttgcaggg ccgcttagtc
      181 cgggaggagg tgaatatcgg aatgttaaag aattatgagg cactttatat aattgatgct
      241 cagcttgagg aaaatgctat aaaagagact gttgaaaagt tcagcaccct gattagcaca
      301 tcagcacaat taaaaagtac cgatgaatgg ggtaagagaa gactggctta cgaaatcaac
      361 aagaagaatg aggggtatta tgtgctgact gagtttagcg cagaacctga tttcccccag
      421 gaattggaga gaatatttaa gattaccgaa ggaataatca aatatctgat tgttaatcgt
      481 gataattaaa tatttgaata ccaatcggta gatgctaatt agcggaccta aaaaaattta
      541 agtagggtga ttttgttatg aataaagcca ttttattggg tagacttact aaagaccccg
      601 aactgaggta taccagcact aataatacgg ctgtttgcaa ctttacaatt gctgtaaacc
      661 gcagattttc caaaccggga gaagagaaac aggcagattt tatacctgtt gttgtgtggg
      721 acaaacttgc tgaattttgc ggcaaatact ttgcaaaagg acgtcaagta gcggttgtag
      781 gacgcataca gacaagaaca tgggatgata atgaaggtaa acggcattat gttaccgagg
      841 ttgtggcaga agaagcatat tttgcagata gtaaacctga taatagcggt atgcctggta
      901 tgccccgcac aaatgaaagt gcaagtccgg cttttgaggg tggagcagca tccggaaggg
      961 atgatgggtt ctaccaaaca gaagaggatg acgaactgcc gttttagtaa aaaattcagc
     1021 aaaacagaga cgatgaatac taacataatg ttataagaag ggaggttttc atatgccgac
     1081 agggccgaaa agagaaagag gatccgggag agattcttat gataagggtg actccaaggg
     1141 cggtatgaga atgagaaggc agaaaaagaa agtatgtgtt ttctgttcag ataaaacagc
     1201 taatatagat tataaggata ttgccaaatt aaagaaattt atttctgaaa gaggtaaaat
     1261 acttccgaga agaatatccg gaaactgtgc caaacatcag agagaattga ctcgtgccat
     1321 caaacaggca agacatatag ctttgctgcc ttatatatct gagtaaaagg ttaaatcagt
     1381 cagtatgaac ggtgatgagc cggtaatcat taggtaatga actgataaaa atcttatgtc
     1441 aaagacgcaa tgttattttg ggttttggcg taggcatgcg ataaattaat gatatataat
     1501 aatatatatt ggatatatat tgaaatgcat atgtaaacaa gaaatgagcc ggtattgtat
     1561 ttcttaatac tttatatgag taatatgagt agtagaagaa tcaccggctt gttttttcat
     1621 atgtaatatg taatatgcaa tatgcaatac gtaaaatgca atatacacat gaactacagg
     1681 ttatgtttga tgtttgcggg gtgggtatgg attatgatta aaataggata tttagggcca
     1741 aaaggcactt tttcacagga agctgtaaaa aagtatataa atgagttaat aatcgagggg
     1801 tcaataaatt atgcgaacaa ttaccgtgag gtcaaagaag tcaaaggaaa taagtccgac
     1861 agtaatagta tcatggacaa tagtgtcaaa gaaattaaga ttagcgaaga cacatccggc
     1921 atcaatacgg ccaataataa tggactcaat atcaatgcag ccaatatcaa tgcgcccaat
     1981 atcaatgcag ccaataataa tgcattctgc attaacccgg caattgataa cgcactcagt
     2041 atcaataagg caaatgacaa cgaggcaggc atcagtaaaa ccaataatac caggaacttc
     2101 gactatactg tcaatggcaa taagtacagt aatgacaagc ataatgagga cgtttacagc
     2161 gatgacaatg ataacaatga gaacattaaa aataaaagga taaatgtagc cgatacaatt
     2221 tgtactaaca taagtataaa tgcatattcg gagcaggcct ataataatat tggagatatg
     2281 cttgctgcca taaagcaggg ttatattcat gaagcagttg tacctataga aaattccctt
     2341 gaaggtcctg tgaatgaaac tctggatctg atggcatccg acatggattt aaaaataaag
     2401 ggggaagtaa ttattcccat taggcagaat cttctggtta ggaagggtac ttgcattaat
     2461 gatattaaat atatactgtc tcacccccaa cctgttggac agtgtagaga ctacattttt
     2521 aagaattttc ccgaggctga aataaaactg gtatatagca ctgcaggagc agcagaaatg
     2581 gtatctaaag atgaatatgg ttttacagcc gcagcaatag ggtccttaac agctgctgat
     2641 gtatatggac tggaaatttt ggctaaaaat atacaggatg gagaaaataa ttttaccagg
     2701 ttcgttatta ttgcaggtga agatagcgga aaaacaggtg atgacaagac atctctggta
     2761 ttttctacgg aaaacaaacc aggaagtctt tatagaatcc ttgaaatatt taacctgtgg
     2821 gatataaata tgacaaaaat cgaatccaga cctgcaaaaa agcagctggg acagtacatc
     2881 ttttttgtag acatagaagg ccatagagag gacgaagatg taaaagatgc acttattatg
     2941 gtgaagcgta agacatcata ctttaaatta ttagggtctt accctgtgtt taaaagaaag
     3001 aaaatgtaga cagcatattt ttgtattatt taggagaggt tgtatgggat ttatggaaaa
     3061 agatatcgat ataaccagaa acataaaaat tatagagcgg ctgaaaagcg aactgcttac
     3121 agatatagca aatctattca gaatattggt aaatggagcc agggaagaaa tccacgaagc
     3181 tatctctgaa gctttatcaa atattattct gatatgttat ttacttggta gaaggctggg
     3241 tgtaagctat aactccatag agatgaaaat ggacggtaaa ataaggctgg gccttgttga
     3301 atctgatgat atggaaaagt attatggtga tctttccgaa ctggccagac accttaatag
     3361 ttccagagct aaaaagaaat aagaggaatt gctatggata acagaaaatt tacacatgtt
     3421 tttatgccgc gtacaagttc atacatatgg ataatattaa ttttgctgtt aacgataaca
     3481 gtattggact ggcgtgtggc tttaccgggc tatctgcttt tttttgtact ggttttttat
     3541 aatttcaggg ctaattacaa gcggcaaaaa gacattataa aatatataga aaatcttaca
     3601 tttaacatag attcagcttc aaaagatacc cttttgaatt tccccatgcc tcttgttgtt
     3661 gtggaactgg atggcacaat aatatggtat aatcagaagt ttcggaaaat attccgggag
     3721 gaagagctcc ttgaaaatac aataagttca tttgtagatg aacttcaccc ttataacatg
     3781 atagatgatg aggttaacat ttttagagaa ataaaaataa atgacaatta ttacagggtt
     3841 cttgggaact ttgttaaaaa ccagggacgg acagatgtta cggaatatat tcttctgcta
     3901 tattttctgg atattagtga tctgatggaa cttagaacaa gatataacaa tgagaaacct
     3961 gtttcaggga taattattat tgataactat gacgaactga tgcagagcac tgacgataca
     4021 gcccggccgc agatccttgc ggaaatagat aaaaaaattg ttcaatggct gggttttacc
     4081 ggaggaatat tgaaaaaatt tgagagagac aagtatttgt ttatggttga atataaatac
     4141 atgaaagagt tagaggacag gaaatatgat attctagata ctataaaaga aattagtgta
     4201 ggaaataaaa ttcccgtaac tctcagtatt ggatttggta taaataatgg aaatttctct
     4261 ggtaatatgt ctgcagcaag tgcatctata gatctagccc ttggaagagg aggagatcag
     4321 gttgttatta aggatggaga taacttcaga ttctttgggg gcaggacagg agaacttgag
     4381 aagcggacaa gggtcaaggc aagggttata gcatatgctt taagggaatt aatagaccag
     4441 tcatatcaag ttatgataat gggtcatgaa aatcctgatg tagatgctct tggcgcggca
     4501 cttggcatat atagaatagc aaaaagcagg gaaaaagatg ccagtattgt gcttagccag
     4561 tcaaatcctg caattgaaag cttgttgtca aggattgaaa agacaggtga atatggcaat
     4621 ttatttatag ggaaaaatga ggctcttgat agaataaaca gcaagacctt gcttgttatt
     4681 gtagatactt acagagccaa gtatacagaa catccggagt tattaaaata taccgaccag
     4741 atagtagtta ttgatcacca tagaagaggt gtcgatttta tacaggaagc agccctgaca
     4801 taccaggaaa tatatgcatc ctctacctgc gagatggtta cggaaatgat acagtatgta
     4861 gataacaagt taaaacttaa ggctatggaa gcggaagctt tgtatgcagg catagtaatg
     4921 gataccaaga actttacatt taaaacgggt gtcagaactt ttgaagcggc ttcattctta
     4981 agaaagcagg gtgtgaatac tcttgctgtt aaaaagttat ttcagaatga cctttcaact
     5041 tttttaaata tatcttcggt agttagagac gcggaaatta tattcgagaa catcgcaata
     5101 tccgtttgtc ccccgaatat aaggaatggg ctgttgatag ctgcaaaatc tgctgatgaa
     5161 atcctgaatc tttcaggtat aaaagcggct tttgttttat gtacggcaaa taatggagtt
     5221 gcaataagcg gaaggtcttt tggtgatatt aatgtccagg ttatactgga aaagctgggg
     5281 ggcgggggac atcagacagt tgcaggtgca cagattgaga atatttcttt ggaggaaggg
     5341 aaagaaaagt taaaatatgt tataataaat actatcaatg aaataaaacc atgaaggtcg
     5401 taagtaggtt ttatatttat gtatttattg aaacggtgtg aagcaatccg attggcggat
     5461 tgcacttatg tta
//
LOCUS       DAOSDD010000270         5453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5354_length_5453_cov_4.999629, whole genome shotgun sequence.
ACCESSION   DAOSDD010000270 DAOSDD010000000
VERSION     DAOSDD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5354_length_5453_cov_4.999629"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK733_17180"
     CDS             complement(<1..351)
                     /locus_tag="PK733_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12300.1"
                     /translation="MNKHEQKPKIGLLSLYIELYDKSSPQKRVGIDAFNKTIINELEK
                     RGLEIIASPVCRIKSEFKSAVKAFEKDEVDAIVTLHLAYSPSLESSDILAATKLPIIV
                     LDTTPTYDFSSEQEM"
     gene            complement(426..1484)
                     /locus_tag="PK733_17185"
     CDS             complement(426..1484)
                     /locus_tag="PK733_17185"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ12301.1"
                     /translation="MKKNITLKDIAQKVGVSIATVDRAIHNRGEINTETKQKIIKTAN
                     ELGYKPNKIARTLVMKKEIKIGVILPLHPKFFWSEIERGIKYVDENIRDFGVKVSYYR
                     IFDNIDTQRYTIEKLISEGINALIIKPFYHETIIEKLNECIKKGIQVVMINDDIKEIN
                     SLFYVGSNDEEAGRTAGQMMSMLLKGKGKIAAVRDSENLSYSLKLRYSAFCKQIGEMS
                     DKLEIIDNMKYLIEITGGYDAYLATKYYIENIPDIAGIYNDSGSIYQVGLAVKNENTE
                     KKIIVIGHEVSPEVNELVVEGYVDAVISQDPFFQGYYSMKNLGDFLIEGKEPEFKQYL
                     SHTDIILKSNIRTRNSMQ"
     gene            complement(1771..2415)
                     /locus_tag="PK733_17190"
     CDS             complement(1771..2415)
                     /locus_tag="PK733_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOJ12302.1"
                     /translation="MDLIQNPVYIVTQRIISPGGEMVIDYPVAVGMPNQAVQSQINQV
                     ILSLVNKIMNEQNRQLIKQGYKDLSKVSIQGWYELKTNERGILSLSIGNYTIAYPAAH
                     GLTIIRSLTFDIITGKAYKLNELFKPESNYVKKLSLIIEKQIKERKVPLLSDFKGIRP
                     DQDYYIADKALVVYFQLYELTPYAYGFPQFPISVYELESIIREDGPLGKMAVNT"
     gene            complement(2626..4353)
                     /locus_tag="PK733_17195"
     CDS             complement(2626..4353)
                     /locus_tag="PK733_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12303.1"
                     /translation="MLKRLPYGISNYKTIALENYIYVDKTDYIEKIENYHSPFLFFLR
                     PRRFGKSLFVSLLQHYYDINEKDRFDVLFKDTYIGQNPTKEKNRYYILKFNFSGVTTD
                     TKEQLEESFINITKLAFNDFTSRYKLTLDYVLKESDINVSAANMLEDFFTSVKNKIDK
                     PIYVLIDEYDHFANELLSFQFNLFADSISKTGFVRKWYEVLKKATETIVKRIFATGVS
                     PITLDSLTSGFNIADNITREEAFNEMMGFTEDEVRQIVKETVRFPMNENDMDALIEEL
                     HKNYNGYLFSEDANKRLFNSDMILYYLKTFIEKKKGPNDLIDQNIASDYGKLGKMFEL
                     KDRKGNMEVLEKILNGEEIVAQITRQFSMEKDFTTDDFKSLLFYLGLLTIDKRVLDSV
                     SLKTPNYVIKGLYYQYYAKKINEETGYDINTSFIKEGIRQIAIEGKNDKFISVIENTL
                     HKLSNRDYINFDEKYIKLIMLSYFMLSNIYMVKSEYEVEDGYIDIALLRREPIDPSYF
                     AILEVKYIKKSEYETYGEEIVVEKKNEAISQIMKYQSSQELRDLSELKKWVTVFVADK
                     CFINMGIHS"
     gene            complement(4546..5235)
                     /locus_tag="PK733_17200"
     CDS             complement(4546..5235)
                     /locus_tag="PK733_17200"
                     /inference="COORDINATES: protein motif:HMM:NF020822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12304.1"
                     /translation="MIQKAWKNVLFNQFHDIMGGCSIKEAYDDAGEFYGESFPVNITD
                     TAAVYEIPYGHIERPANGEEEPGQQWVDVFGTVLKSDSHMDYMDHFGKRDEFCEFMDQ
                     GIHEFGYVLVPHKDNWKESGIVRKAFEFNTRFPNIIEKYHKGVLSQSLSGICILKENI
                     VAVVFKREEDEGAYILRCYETCAKNTEATIEIPLLNRRWKTSFGACEIKTFRIPDDLN
                     KPIEETSLLEY"
BASE COUNT         1588 a         1056 c          747 g         2062 t
ORIGIN      
        1 catttcttgt tcggaactga aatcataggt aggagttgta tccagcacaa taattggaag
       61 tttggttgct gcaaggatat ctgaggattc taatgatggt gaatatgcca ggtgcaacgt
      121 aactattgca tctacctcat ctttttcaaa ggctttaact gcacttttga actcggattt
      181 gattctacaa acaggagaag ctattatttc caaccctctt ttttcaagtt cattgattat
      241 agttttattg aaggcgtcta ttccaaccct tttttgtgga gatgatttgt catacagttc
      301 aatatataat gatagcagcc cgattttagg cttttgttca tgcttattca tttctatccc
      361 ccttataatt tgcatttatt gctattaata atttgctttc tttttatgta gacacatttt
      421 tttgcctatt gcatgctgtt tctagttcgt atattactct tcagaattat atccgtatga
      481 gaaaggtact gcttgaattc aggctcttta ccttctatca ggaagtcacc caggttcttc
      541 attgaataat acccctgaaa gaaaggatcc tggctaatta cagcatctac ataaccctca
      601 actaccagtt cattaacttc tggtgaaacc tcatgtccta taacaataat ttttttctct
      661 gtattttcat tcttcacagc caaaccaact tgataaattg acccactgtc attatatata
      721 cctgcaatat ccggtatgtt ttcgatataa tattttgtag ctaaatatgc gtcatagccc
      781 ccggttattt ctattaaata tttcatatta tcaataatct caagcttatc agacatttcc
      841 cctatctgtt tacaaaaggc tgaatatctt agtttgagtg agtatgagag attttcgctg
      901 tctcttactg ctgcaatttt cccttttcct tttaataaca tactcatcat ttgacctgct
      961 gttctacccg cttcttcatc attagacccc acataaaata aactgttaat ttcctttata
     1021 tcatcattta tcataactac ttgtatacct ttcttaatgc attcattaag cttctctata
     1081 atagtctcat gatagaaagg ttttataatt agcgcattaa taccctcgct tattagtttt
     1141 tctattgtat atctttgtgt atcaatattg tcaaagatcc tgtaatagct aacctttaca
     1201 ccgaaatctc taatattttc atcaacatat ttaattccac gttctatttc agaccagaaa
     1261 aatttagggt gtagtggcaa tattacacca attttaattt cttttttcat taccagtgtt
     1321 ctggcgattt tatttggttt atacccaagt tcattagctg ttttgataat tttttgtttt
     1381 gtttcagtgt ttatttcacc tctattatgt attgctctat caacagtagc tattgaaact
     1441 ccgacctttt gtgcaatatc tttgagagta atgtttttct tcattatata agcctccagc
     1501 ttaatcatat cttatgccgt ttaactacct tatttaacag atatatttaa cgcttattca
     1561 aatcttcggt aacgttaccg ttactaaatt aataataata taataatttg atattgtcaa
     1621 taagaaattt aataagagta ataaatttca ctatttcaat agggacagtt ctcaagcagt
     1681 tcaattatct taatgatttt cgactgtccc catatttaac aattgtccca atacctaaat
     1741 aactgtctcc ataccatgta tactcttaaa tcacgtatta accgccatct ttccaagggg
     1801 gccatcttct ctgataatgc tctccagttc atagacagat atgggaaact gaggaaatcc
     1861 gtaggcatat ggagttaatt cataaagctg gaagtataca actaatgctt tatcggctat
     1921 gtagtagtcc tgatctggtc taatgccttt gaaatccgaa agcagcggaa cctttctctc
     1981 ctttatttgt ttttcaataa ttaaagatag ttttttcaca tagttgcttt caggcttaaa
     2041 taactcattc agcttatatg ctttaccagt aattatatca aatgtgagtg acctgattat
     2101 agttaaacca tgagcagcag gataagcaat tgtataatta cctatggaca aacttaatat
     2161 tcctctttca tttgttttta gttcatacca tccctgaatt gatacttttg ataaatcctt
     2221 gtatccttgt tttataagtt ggcgattttg ctcattcatt attttattta caagacttaa
     2281 tattacctga tttatctgtg attgtactgc ttgattgggc atgccgacag caaccgggta
     2341 gtcaataacc atttcaccac cgggagatat tatcctctgc gttacgatat atacggggtt
     2401 ctgtataaga tccataatat aaatacacct tccatgatat ttattactgt tatagtatat
     2461 tcatataaca tgaaaggtgt tagctttaaa ggtttgaaaa cctgttttta ttacagacta
     2521 tattaagcgc tgatatgatt ctgctctgta atcatttgcc gcactgcctt ttatatatta
     2581 tttatcttgc aatatctacc cggatattcc cggcaaatta ctattttacg aatgaattcc
     2641 catatttatg aaacatttat ctgctacaaa tacagttacc catttcttaa gctcagacaa
     2701 atccctaagc tcctgtgagc tttgatattt cataatctgg cttattgcct catttttctt
     2761 ttctactact atttcttcac catatgtttc atattctgat tttttaatat acttaacctc
     2821 aagtattgca aaatatgaag ggtctattgg ctcccttctc agtaatgcta tatctatata
     2881 tccatcttcc acctcatatt ctgattttac catgtaaata ttgcttaaca tgaagtatga
     2941 aagcataata agctttatat atttttcatc aaaattaata taatcccggt tggaaagctt
     3001 gtgcaatgta ttttcaataa ctgatataaa tttatcattt ttgccttcaa tagcaatttg
     3061 ccttatacct tctttgataa aggaagtatt tatatcatag cctgtttctt catttatttt
     3121 tttagcgtaa tactgatagt ataagccctt gataacatag ttaggcgttt ttaaacttac
     3181 gctatccaat actcttttat ctattgtaag aaggccaagg taaaatagca gggatttaaa
     3241 atcatcagtt gtaaagtctt tttccatgct gaattgtctg gttatctgcg ctactatttc
     3301 ctcaccattt agtatctttt ccagtacttc catattgcct ttccggtctt ttaactcaaa
     3361 cattttacca agctttccat agtcgctggc aatgttttgg tctattaaat cattgggacc
     3421 ttttttcttt tctataaaag ttttaagata atatagtatc atgtcactat taaatagcct
     3481 tttatttgca tcttcagaga ataaataccc gttataattt ttgtggagtt cttctattaa
     3541 tgcatccata tcattttcat tcattggaaa ccgcactgtt tctttaacta tctgcctcac
     3601 ttcatcctca gtaaaaccca tcatttcatt gaaagcttct tcacgagtta tgttgtcggc
     3661 aatattaaac ccactggtaa ggctatctaa ggtaattggg cttacccctg ttgcaaatat
     3721 acgctttacg attgtttcag tagctttttt taagacttcg taccactttc ttacaaagcc
     3781 ggttttgctt attgaatctg caaacaaatt aaattggaag cttaaaagtt cattagcaaa
     3841 gtggtcatac tcatcaatca aaacatagat aggtttatca attttattct ttacactcgt
     3901 aaaaaaatct tccagcatat ttgcagctga tacatttata tctgattctt ttagcacata
     3961 atcaagagtt agcttataac ggcttgtaaa atcattgaat gccagctttg tgatattaat
     4021 aaagctttcc tccagctgtt cttttgtgtc agtagtaact ccggaaaaat taaattttaa
     4081 aatatagtat ctatttttct ccttcgttgg attttgtcct atgtaagtat ctttgaacaa
     4141 aacatcaaag cgatcctttt catttatatc atagtagtgt tggagcaaag acacaaacag
     4201 gcttttgcca aaccttctcg gtcgcagaaa aaataggaat ggtgaatggt aattttcaat
     4261 tttttctata tagtcagttt tgtctacata gatataattc tctaatgcaa ttgtcttata
     4321 attactaatt ccatatggca atcttttcaa catagcccgc tcctttcaga atctgtaaaa
     4381 gctgttgagg atatttttca atcatttttt accggaagct ttgattacaa ttataaacga
     4441 ttttcttaat tttaacaatt agaaataata gcttcaggga gcattttctt cctacaatac
     4501 aaaatataga aaaatgcgtc tattaacagc tcggaatatc aaaaactaat actctaataa
     4561 gctggtttct tctatgggtt tgtttaaatc atccggtatt ctgaaagttt tgatttcaca
     4621 cgccccaaat gatgttttcc accttctgtt caacaatgga atttctatag ttgcttccgt
     4681 atttttcgca catgtctcat aacatctcag tatgtatgcc ccctcatctt cctctctttt
     4741 gaaaacaact gcaacaatat tttcttttaa tatgcagatt ccgctaagac tctgcgacaa
     4801 aacgccttta tgatattttt caattatgtt ggggaacctg gtgttaaact caaaagcttt
     4861 tctgacaatt ccagattctt tccaattgtc tttatgcggc accaatacat atccaaactc
     4921 gtgaattcct tgatccataa attcacaaaa ctcatctctt tttccaaagt gatccatgta
     4981 atccatgtgg gaatctgatt tcaatactgt tccaaaaaca tctacccatt gttgaccagg
     5041 ttcctcttct ccatttgcag gtctttcaat atgaccatat gggatttcat acaccgcagc
     5101 agtatcagta atattaaccg ggaaagactc tccatagaat tctcctgcat catcataagc
     5161 ttctttaatg ctgcaaccac ccataatgtc atgaaattga ttgaacaata catttttcca
     5221 tgctttttga atcatattac acttcatgta ttctattgaa atctattcta acatatttcc
     5281 ctgcaactac agatttttat tactataaat ttatttttta ctaataatat aaacgtgaac
     5341 tacaccacta ttgaaatagc gggcttcggg ttcccgatga aattatggta attccagcgg
     5401 tacacctccg ggttatccaa ccccactact gctggattgg gcttgccaat cct
//
LOCUS       DAOSDD010000271         5445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5364_length_5445_cov_5.266605, whole genome shotgun sequence.
ACCESSION   DAOSDD010000271 DAOSDD010000000
VERSION     DAOSDD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5364_length_5445_cov_5.266605"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2523)
                     /locus_tag="PK733_17205"
     CDS             complement(<1..2523)
                     /locus_tag="PK733_17205"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ12305.1"
                     /translation="MKTIFNRKFFKRSIRNTITWFVMLAMLFTNIGQVVMPAVHAMDM
                     AGDTYAQIQSENLLEAEGEASGEGLGQPTEQADEPEAGGEAAEGAGEDLEQPVEQAGE
                     SEVGKESEGAEGAGESEQPTEQPIGQNAEPGAEEEAEDIEEAGEEAEEAGEETEAANG
                     EAEEAGEGEETGTDNGVTGETGVNGETGETGDIGDGLLDEDKDGLNKENPNIRAMLLN
                     NMSYEEGSIQNPIPLEIGNIIELEMEAYTARYYSITAPEDGCYYARTYTPAQGDAAQA
                     SILIGYANEYDTFVQEGISGYVFDNNDNYIYYAKKDLTETDQKLIGIYTGNTRGYVRF
                     NVYKEFTPEQATPSVPSGVYSAPVGNVTLAAQEGMNIYYSTYPWIGSDLTQYNSYTSE
                     SIISNPDTLKVIIEDPVTKKQSGVLSLNYIIVDGTIVPSSEPGSTGGLTLSIGGDISS
                     KKFLVQTTAESSYSLYIYNTDGTPASFADSNLNIFLRDQDDNWITSSGPINMIPEGVA
                     VLGFEAEPDTSYFLEINNYSEEPEEYIFKLLENPPAQPQVSEVVTPITEPVYVSLIAE
                     EGATIYYYLETWDAIFPRPTPTYIEYTSPILIDRSLALMSYCTKNGNSSALANFYFGD
                     IEAPGGPNILPEENRQLPGTKVTISGADTGDIIYYTTDGSNPYNSTTKQVYSDESDIQ
                     ISDNLVIRAIIQNSSGIYGYASYKHYEEGIPIPTVNFVYEVDDEGGYYKIEISTGASE
                     GEIYYTIDGSDPQISGTPYTNPIIIRKNTELRAVTKLNDDFSVENKHDFSLSYERKVE
                     LDILYDGYLPKYSSYEWFKFTIPAVGKYSFEIIPAESNRFSEV"
     gene            complement(2541..2921)
                     /locus_tag="PK733_17210"
     CDS             complement(2541..2921)
                     /locus_tag="PK733_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12306.1"
                     /translation="MYGSMDGFSLKPHVHLIWHIGHVEDIMVFQACELKLKTGFFKTE
                     LYNLSIYDDKIVLTPKENTVLQEIVITNENLNSISLVNKSTDFFEIEIYTQQGMFIGN
                     ISASFNSKKLLRILVEKFDRKILI"
     gene            3125..4669
                     /locus_tag="PK733_17215"
     CDS             3125..4669
                     /locus_tag="PK733_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOJ12307.1"
                     /translation="MPFHEKLNLLMNITNTTNSILANAVSLDPSFVSRLRQGTRYPSK
                     NENYIKPIAFFLAKHISEEYQQTALSDALNIPVKNVPTDVEALSDLIYKWFYDYSLHD
                     AATVQGFIDTLTHPTLKKISVGIGNNVEVKDYSKSNRIDASVFYGIEGKQSAALEFLS
                     LVANNQNPQTLLLFSDEDIEWLTLNPDFITRWSILLMQIASKGNKIKIIHTVGRDLSE
                     MLSAIKQWLPLYMTGAIEPYYYPKKRDGVFQRTLFIAPDTAAVVSGSVGKKIDNTANF
                     LFKDKKTILALADEFYNYFSLCRPLMKIFTSSETRKFFATLQEFEGEDSNTIITTDTL
                     STITMPSGIFNSIFARTKTKLKNDFLFEQELRVKNFEESLLIHKFTETINMPDLEEIR
                     LGKIKIAFSDMFSETQLFYTIEEFISHLENIIRLLKEYKNFYVSLKTDYSISGFMLYV
                     KEDVGVLVAKSSMPTVMFAINESNLTAAFWDYLEPIIRGYSKSPSDKINVIAKLEEAV
                     RELRSR"
     gene            4819..>5445
                     /locus_tag="PK733_17220"
     CDS             4819..>5445
                     /locus_tag="PK733_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ12308.1"
                     /translation="MDNFFSILKECALCPRKCNVNRIAGEKGVCRASASVKVSLASLH
                     KWEEPCISGDNGSGTVFFTSCNLKCVFCQNYEISQKDYGKEVTIEHLAEIFLNLQGQG
                     ANNINLVSPTHFMPQIKEALKIAKLNGLNIPVVYNSNGYETVDALRFMEGMVDIYLPD
                     LKYYNDEYGTKYSSAPHYFQYATKAILEMYRQVGSPEFDDNGLIKNGLI"
BASE COUNT         1627 a         1069 c          952 g         1797 t
ORIGIN      
        1 tacctccgaa aatctgttag attcagccgg aataatttca aaactgtatt tcccgacagc
       61 gggtattgta aacttgaacc actcataaga tgaatattta ggtaagtatc cgtcatataa
      121 aatatccagt tcaactttcc gctcatatga taaagaaaaa tcatgtttgt tctcaacgga
      181 aaaatcatca ttcaattttg tgacagctct aagctcggtg ttctttctta ttattattgg
      241 gtttgtatat ggggttccgg atatttgagg gtcggaacca tctatagtgt aataaatttc
      301 tccttccgat gctccggtag atatttctat tttataatac ccgccttcgt cgtcaacttc
      361 atatacaaaa ttaactgttg gtatggggat accctcttca tagtgtttat atgaagcata
      421 cccataaata cccgaagaat tttgaatgat ggcacgtatt actaaattat cacttatttg
      481 tatatcagat tcatctgaat aaacttgctt tgtagtgcta ttataaggat ttgaaccatc
      541 agtggtataa tatattatat cacctgtgtc agctccggat attgtaactt tcgtacccgg
      601 taattgtctg ttttcttcag ggagaatatt tggacctcca ggagcttcta tatcaccaaa
      661 gtaaaaattc gccagagcac ttgaatttcc atttttagtg caatatgaca ttaaagctaa
      721 agacctatct atcaatattg gtgaagtgta ttctatataa gttggtgtag gtctggggaa
      781 aattgcatcc catgtttcca gataatagta aattgttgcg ccttcttctg ctataaggct
      841 tacataaaca ggctctgtaa ttggagtaac tacttcactt acctggggct gggcaggtgg
      901 gttttcaagc aatttaaaaa tatattcctc aggctcttcg gaataattat ttatctctag
      961 aaaatagctt gtgtcgggct ctgcttcaaa gccaaggaca gcaactcctt ctggaatcat
     1021 atttataggt ccgctgcttg taatccaatt atcatcctga tctcttaaaa atatgtttaa
     1081 gttactgtcg gcaaaacttg ctggtgttcc atcggtgtta tagatataaa gggagtagct
     1141 gctttctgct gttgtttgta caaggaattt cttagaagat atatctcctc caatacttaa
     1201 agttaatccc cctgtacttc cgggttctga ggagggaaca atagtcccat caactataat
     1261 gtagttaagg gacagcacac cactctgctt tttagtaacc ggatcctcta ttattacttt
     1321 gagggtatcc ggattactta ttatagattc tgaagtataa ctgttatact gggttaaatc
     1381 actgccaatc cagggatatg tactataata aatattcata ccctcttggg cagctagtgt
     1441 tacatttcct acaggagcgc tgtatacacc tgaagggact gaaggtgttg cctgttcagg
     1501 agtaaactct ttatacacat taaatcttac ataccctctg gtatttcctg tatatattcc
     1561 tattagtttc tgatcagttt cagttaaatc tttttttgca taatatatgt aattgtcatt
     1621 attatcaaat acatacccgg atattccttc ctgaacaaaa gtatcgtatt cattggcata
     1681 tcctataagt atgcttgcct gagctgcatc gccctgtgca ggagtataag tccttgcata
     1741 atagcagcca tcctcaggag cagttatgct gtaataacga gcggtatatg cttccatctc
     1801 cagttcaatt atattcccta tttctaaagg tatgggattc tgtatgcttc cttcttcgta
     1861 agacatgtta tttaacagca tagctctaat attgggattt tctttgttca atccatcctt
     1921 atcctcatca agcaagccat ccccgatgtc tcctgtttct ccggtttctc cgtttactcc
     1981 tgtttctccg gttactccat tgtcggttcc agtttcttca ccttctccgg cttcttcggc
     2041 ttctccattg gcagcttcgg tttcttctcc ggcttcttcg gcttcttctc cagcttcttc
     2101 aatatcttca gcttcttcct cagctccggg ttcggcattt tgccctatcg gctgctctgt
     2161 tggctgctct gattcacccg ctccttccgc tccctcggat tcttttccga cctctgattc
     2221 gccagcctgt tccaccggct gttctaaatc ttccccggct ccttctgcag cttccccacc
     2281 ggcttccggt tcgtcagcct gttccgtcgg ttgccctaaa ccttcaccgc tagcttcgcc
     2341 ctctgcttct aaaaggttct cagactgaat ttgggcatag gtatcccctg ccatatccat
     2401 agcatgaact gcaggcataa ctacctgtcc tatattcgta aacaacatag caagcattac
     2461 aaaccacgta atggtgttgc ggatagacct cttgaagaat tttctgttaa atattgtttt
     2521 cataacaatc ccctttacat ttaaattagt atttttctat caaatttctc aactaatatt
     2581 ctaagaagct tcttcgaatt gaagctggct gatatatttc caataaacat gccttgttgc
     2641 gtatagatct ctatttcaaa gaaatctgta cttttattga caagactaat agagtttaaa
     2701 ttctcatttg ttataactat ctcttgaagc actgtgtttt cttttggcgt taacactatt
     2761 ttatcatcat aaatagacag attgtataac tctgtcttaa agaaccccgt ttttagcttt
     2821 aattcacaag cctggaaaac cataatatcc tccacatgcc caatgtgcca aataagatgt
     2881 acatggggtt ttagactaaa gccgtccata ctgccataca tctaaataag cacttcaatt
     2941 attactatat caaaattgac cattcatgca atgtcagaag gcaatctgtc aatgacagat
     3001 tcttgacata atatgtgcca aaatatattg ctagaactaa attaaatatg ttatactgaa
     3061 tacaatcaag gaaataatct tgaccacatt aatgtccata taaaaaataa gggggagagg
     3121 tcacttgcct tttcatgaga aacttaatct tctaatgaat attaccaata caacaaacag
     3181 tatacttgct aatgctgttt ctcttgaccc ctcctttgta agtcgtttga gacaggggac
     3241 cagatatcct tcaaaaaatg agaactatat taaaccgatt gctttcttcc ttgcaaagca
     3301 tatttccgaa gaatatcaac aaaccgcatt aagcgatgca ttgaatatac cagtaaaaaa
     3361 tgttcctact gatgtagaag ctctttctga tttgatatat aaatggttct atgactattc
     3421 tttgcatgat gcagcaacgg tacaaggctt tatagatacc ctgactcatc caaccttaaa
     3481 aaaaatatct gtaggtatag gtaacaatgt tgaggtgaaa gattactcaa aatctaatag
     3541 aattgatgct tcagttttct atggtataga agggaagcaa agtgcggcct tagaattttt
     3601 atccctggta gcaaataatc aaaaccctca gacacttctt ttattcagcg atgaggacat
     3661 agaatggcta actttaaatc ctgattttat tacaagatgg tcaatacttt taatgcagat
     3721 tgccagtaaa ggtaataaaa taaaaattat tcataccgtt ggcagggatc tatctgaaat
     3781 gctttctgca attaagcaat ggcttccatt atatatgaca ggagctatag agccatatta
     3841 ctatcctaag aaacgtgatg gtgttttcca gagaacgtta ttcattgcac ctgatactgc
     3901 tgcggtggta tcaggttctg tggggaagaa gatagataat acggcaaatt ttctttttaa
     3961 agacaagaaa actatccttg ctctggcaga tgagttttat aattattttt cactttgtcg
     4021 cccacttatg aaaatattca cttcatcaga gacaaggaaa tttttcgcaa ctttacagga
     4081 gtttgaggga gaagattcta atacaataat aacaactgat acactttcca ctataacaat
     4141 gccttccggg atatttaaca gcatatttgc ccgtacaaaa actaaattaa aaaatgattt
     4201 cttattcgag caagaactca gagtaaaaaa ctttgaggaa agtctgctaa tacataaatt
     4261 tacagaaact attaatatgc cggatttaga agaaatacgg cttgggaaaa ttaaaatagc
     4321 tttttccgat atgtttagtg aaactcaact attttatact attgaagagt ttatctccca
     4381 tttggaaaat ataattcggc ttcttaaaga gtataagaat ttttatgtct ctcttaaaac
     4441 tgattatagt atttcaggat ttatgttgta tgtaaaagaa gacgtcggcg tgctggtggc
     4501 aaaatcttct atgcccaccg ttatgtttgc tattaatgaa agcaatttaa ctgctgcttt
     4561 ttgggactat ttggaaccaa taatcagagg ttatagtaaa tctccatcag ataaaataaa
     4621 tgtaatagca aaacttgagg aggctgtccg ggaattaaga tcaagatgat taagccccac
     4681 taagccggta aaccccaccg agcccctggc gcattttaca gcagcattcc tctttattaa
     4741 aatacatcca ttgacaaatc acctaatctc aatataatta attcttgtaa ctattatgta
     4801 tcaaaggaca gatggattat ggataatttt ttcagcattc tcaaggaatg tgctttatgt
     4861 ccacggaaat gcaatgtcaa tcgtatagca ggtgaaaaag gtgtatgcag agcatcagct
     4921 tcagtgaaag tatctcttgc atcccttcat aagtgggaag aaccgtgcat aagcggtgat
     4981 aatgggtctg gtaccgtatt tttcacaagc tgcaatctaa aatgcgtgtt ttgtcaaaac
     5041 tatgaaatta gtcagaagga ttatggcaag gaagttacta ttgagcatct tgcggaaata
     5101 tttttaaatt tgcaggggca gggagctaac aatatcaacc ttgtatcccc tactcatttt
     5161 atgcctcaga taaaagaagc attgaagata gcaaaactta atggcttaaa tatacctgta
     5221 gtgtataact ccaatggata tgaaactgtt gacgctcttc gttttatgga agggatggtt
     5281 gatatatatc tgcctgatct taagtattat aatgatgaat atggaacgaa gtattcaagt
     5341 gctcctcact atttccagta cgcgacaaaa gcaatattag aaatgtacag gcaggtcggt
     5401 tcacctgaat ttgatgacaa tggactaata aaaaacgggc ttatt
//
LOCUS       DAOSDD010000272         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5401_length_5406_cov_3.031209, whole genome shotgun sequence.
ACCESSION   DAOSDD010000272 DAOSDD010000000
VERSION     DAOSDD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_5406_cov_3.031209"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1887
                     /locus_tag="PK733_17225"
     CDS             37..1887
                     /locus_tag="PK733_17225"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOJ12309.1"
                     /translation="MGILIVGKNKVNKLILDRFNSENLKPIIIENINNIKKIEGEVGS
                     FNITLTDRNILATHIIITEEPVLASFPVESIKKFSTDFNSGFFSSTSSDSGSKSNNNS
                     GFNHEHISDSDIDFDIEHLPIFNLEQLEPGNDTSGIFDICSMILKNKKSPIVFIMDYP
                     EESQAYSTPIVLRKAIEFAKKKRKVFCLMKFIRTAGSEEGSLERLYRDARNSGVTFIK
                     YSNVTINYNMEQSIFKINLAHKIGSTAIETDFLISSGFLVSGENLNKISELLRLKLDK
                     DGFANGSDSFLYPVLTSRKGVFLLNTKLLPGGDEELENCINFTMSKIKEDIKYFSLGK
                     RDSESRFYAKVDSEKCAFCYTCYRACPHHAMSPDYENSIMKCLKNACYGCGICASICP
                     ASAVNMIIDEAILHDDTKSEIKSEIKDDIMSEIKSEIKDDIKGETKSETKDNIEIDDI
                     RSCKSDMGDNESGTLKIICCENSGEIAVRKALKDIDVTCSECKERKELYIEPEKGLYN
                     RIEVMPVPCGGEISTEIILGSLKSFEKVLVMTCIDEACKHFEGNKRARLYVERAKKFL
                     RDSGLDDKRIEYIQVSHAMPYVAGEHIRLLIDKPLIDKPLIDKAVDRQSC"
     gene            1939..2607
                     /locus_tag="PK733_17230"
     CDS             1939..2607
                     /locus_tag="PK733_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ12310.1"
                     /translation="MIVAEQKPIEELISYIKDLSKIVIAACGTCVTVCMAGGEKEALK
                     LASILKVKRLDENRHIDIKVVTPKRQCDDEFLEDIAFEFEDCEAILSLGCGAGIQFMA
                     EKFKGKPVLPGVNTKFMGVTRDIGVWTEMCQGCGDCILEKTGGICPVTRCSKSNFNGP
                     CGGSASGKCEIDPDTDCGWQLIYDRLKALGKLDNLYEIIPPKDWQSSRDGGPRKVIQK
                     EVIL"
     gene            2715..3668
                     /locus_tag="PK733_17235"
     CDS             2715..3668
                     /locus_tag="PK733_17235"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOJ12311.1"
                     /translation="MTGKISEIKPDNISDTISNLQRILEAGHFSVTAEIGPPMSANAD
                     FVRGKARDLAGLVDAANVTDNQTAIVRMSSVAASYLIQQEGMEPVLQMTCRDRNRIAI
                     QSDLLGAYALGLRNVLCLSGDHQSFGNHPQSKNVYDIDSIQLINALRIMKEEKTFMCG
                     RTTKTPAEFFIGATANPFADPEELQFIRLHKKVAAGAQFIQTQAIYDLEKFSTWMEKV
                     RKEGLHEKTYILAGILVNKSLKSIEMTKKVAGMVVPDIYVERMKKAASQEEEGVRIAL
                     DLIGEIRKIEGIKGIHIMAVGWESIVPKIVEKAGFLPRPVL"
     gene            4005..4445
                     /locus_tag="PK733_17240"
     CDS             4005..4445
                     /locus_tag="PK733_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ12312.1"
                     /translation="MSGANSMQEYASLDTGFKNQIASRPGGEKVQNCYLCGTCTAGCP
                     VSSLYDDYNPRRFMRMILLGMKEDVLSSPEIWQCSQCHICVAHCPQDVRFADIVRVLR
                     NMAVEEGYIPESILKEIESIDIDLKKQRIMKVKELTSEKMHKQK"
     gene            4460..>5406
                     /locus_tag="PK733_17245"
     CDS             4460..>5406
                     /locus_tag="PK733_17245"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOJ12313.1"
                     /translation="MRKPVLVLGGGIAGIQASLDLAEMGIPVFIVEKTPSIGGRMAQL
                     DKTFPTNDCSACILAPKVTSCFNHPLIKTFTWSEVLDIKGEAPDFTAVIKKKARFIDA
                     NKCTGCGECTLKCPIEAKNEFDMGIGKRHAIYKPFAQAVPNKVAIDKKGTSPCKFNCP
                     AHIDAHGYVALIGQGRYKEALELVRKTTPFAGVLGRVCFHPCENACSRQYLDSPISIA
                     GLKRFIADYEVSQGIKPQVRLNTGLNTNTGVNTGVNTGINADLNTSENAKKKVAVIGS
                     GPAGLNCAYQLTLMGYKVSIFEALEEPGGMLKVGIPDYRL"
BASE COUNT         1896 a          739 c         1209 g         1562 t
ORIGIN      
        1 tattatttcc tgaacatata agatgcgggg ggaaaaatgg gtatattaat cgttggtaaa
       61 aataaagtaa ataagttgat attggacagg tttaatagtg aaaacctgaa accaattatt
      121 attgaaaata ttaataatat aaaaaagatt gaaggagaag tgggaagttt taatataact
      181 ttaactgata gaaatatatt agcaacccac ataattatta ctgaggaacc ggttcttgct
      241 tcatttcccg tagagtcaat aaaaaaattc agtactgatt ttaattctgg ttttttttct
      301 agcactagtt ctgatagtgg ttctaagtca aataataatt ctggttttaa ccatgaacat
      361 atttctgact cggatattga ttttgatatt gaacatttac ccatctttaa tctagaacaa
      421 ttggaacctg gaaatgatac ctccggtatt ttcgatattt gcagtatgat attaaaaaac
      481 aaaaaaagtc ctattgtttt tattatggat tatcctgaag aatcacaagc atatagtact
      541 cccattgtat taagaaaagc tattgagttt gcaaaaaaga aaagaaaagt attttgcctg
      601 atgaagttta taaggacagc cggttcagaa gaaggaagcc tggaaagatt gtacagggat
      661 gcaagaaatt caggagttac atttattaaa tattccaatg taaccataaa ttacaatatg
      721 gaacaaagca ttttcaaaat aaatctagcc cataaaatag gaagtactgc catagaaact
      781 gattttctaa tatcatcagg atttcttgtt tctggagaaa atttaaataa aatatcagaa
      841 ttgttaaggt tgaaacttga caaggatggg tttgccaatg gaagtgatag ttttctatat
      901 ccggtattaa caagtcgtaa aggggtgttt ctattaaaca caaagctcct accgggtgga
      961 gatgaagaat tggaaaattg tattaatttt accatgtcaa aaattaaaga agatataaaa
     1021 tattttagtt tagggaaaag ggattccgaa agccgtttct atgcaaaagt ggatagcgaa
     1081 aaatgtgcgt tttgttatac ctgttataga gcgtgtccac atcatgcaat gtcgcctgac
     1141 tatgaaaatt ccataatgaa atgtttgaaa aatgcatgtt acggatgtgg gatttgtgct
     1201 tctatatgcc ctgcaagtgc tgtaaatatg attattgatg aagcaattct gcatgatgat
     1261 accaagagtg aaatcaagag cgaaatcaag gatgatatca tgagcgaaat caagagtgaa
     1321 attaaggatg atatcaaggg tgaaacaaag agtgaaacaa aggataatat agaaattgat
     1381 gatataagaa gttgcaaaag cgatatggga gataatgaaa gcggtacttt aaaaataatt
     1441 tgttgcgaaa attcagggga aatagctgtt agaaaagcat tgaaggatat agatgttacc
     1501 tgtagtgagt gtaaggagcg taaggagctc tatatagagc cggagaaagg gctatataat
     1561 agaattgagg ttatgcctgt tccctgtggt ggcgaaataa gtactgaaat aattcttggt
     1621 tcgttaaaaa gttttgaaaa ggtattggtt atgacttgta ttgatgaagc ttgcaagcat
     1681 tttgagggca ataagagagc cagactttat gtggagaggg caaagaaatt tttaagggat
     1741 tcaggacttg atgataaacg aattgagtat atacaggtat cccatgctat gccttatgtt
     1801 gccggtgaac atatacgttt attgatagat aagccattga tagataagcc attgatagac
     1861 aaagctgttg atagacaaag ctgttgatga aaagcagtgc agataatcca gtaatataga
     1921 gaagaaggtg attttgcttt gatagttgcc gaacaaaaac caatagagga gttaataagc
     1981 tatattaaag acttaagcaa aattgtcatt gcagcttgtg gtacatgtgt tacagtttgt
     2041 atggccgggg gagaaaaaga agcattgaag cttgcttcta tcctgaaggt taaaaggctt
     2101 gatgaaaata ggcatattga tataaaagtt gtaacaccaa agagacagtg tgatgatgag
     2161 tttttggaag atattgcttt tgaatttgaa gactgtgaag ctatattgtc attaggttgt
     2221 ggagcaggga tacagtttat ggcggagaaa tttaagggca agcctgtact tccgggtgtt
     2281 aataccaagt ttatgggagt gactagggat ataggggttt ggactgagat gtgccagggt
     2341 tgtggtgatt gcatcctgga gaaaacaggt ggtatatgcc ctgtgacaag gtgttcaaaa
     2401 agtaatttta acggaccttg tggaggatca gctagtggta aatgtgagat agatccggat
     2461 actgattgtg ggtggcaatt gatatacgac aggcttaaag ctcttgggaa attggataat
     2521 ttatatgaaa taataccgcc taaagattgg caaagcagca gagatggcgg acccagaaag
     2581 gtcatacaaa aggaagttat attataagct gtaagctttt ttagcgtata gtaatttata
     2641 aaaaacctaa tatttgggag ttaaattact gtacgctggg gtttgggcgt gcaagtttta
     2701 atagggaggt taaaatgacc ggtaaaatat cagaaataaa gccagataat atatctgata
     2761 caataagtaa tctgcaaagg atattggagg caggtcattt ttctgtgact gctgaaattg
     2821 gtcctccaat gagtgcaaac gcagactttg tccgcggaaa agcacgtgat ttagcaggac
     2881 tggtggatgc ggcaaatgta accgataacc agacggctat agtcagaatg tctagtgttg
     2941 cggcttcata tttaatacaa caagagggta tggagcctgt tttgcaaatg acatgccgtg
     3001 acaggaatcg gattgcaatc caaagcgacc ttttaggcgc ctatgctctc ggccttagga
     3061 atgtcctgtg cctgtctgga gatcatcagt cttttggcaa tcatccgcaa tcgaaaaatg
     3121 tttatgacat agattccata caattaataa atgctttaag aataatgaag gaagaaaaga
     3181 cttttatgtg tggcagaact accaaaacac cggctgagtt ttttattggt gcaactgcaa
     3241 atccttttgc agatccagaa gaacttcagt ttataagatt acacaaaaaa gttgctgcag
     3301 gagctcagtt tattcaaaca caggcaatat acgatttgga gaaattttcc acttggatgg
     3361 aaaaagtaag aaaagaaggc ttgcatgaaa aaacttatat tttggcaggt attttggtta
     3421 ataaatcctt aaagtctatt gaaatgacaa agaaggttgc cggtatggta gtacctgata
     3481 tttatgttga aaggatgaaa aaagctgctt cccaagaaga agaaggtgtt agaattgctt
     3541 tggacttaat tggcgaaata agaaaaattg aaggcataaa aggcatccat ataatggcag
     3601 taggttggga aagcattgta ccaaaaattg tggaaaaagc aggttttcta ccaaggcctg
     3661 tattgtagat aattatatat atacttacgc gaaatggtac ggtattgcaa aagcaaaagg
     3721 aagtaattat atatatactt actgaatttg gctgtaaaag ataaaccaaa gtcttcaata
     3781 acaggtaaat aacaggtaag taacaggtaa ataattagat tatacattag attaaccaaa
     3841 gttttgcatt cttattataa aacctgattt aaaccgctta tatctaaaca tgaaagcagc
     3901 tgattgcagg ctttcaacat aaatgcaaaa ctatattaaa ttatataaat gtgaaagagt
     3961 aatgctgtta acatattgtg aacatattga atggagtgat tagcttgagt ggagcgaata
     4021 gcatgcagga atatgcctca cttgatacag ggtttaaaaa tcagatagct tcgagacccg
     4081 gaggagaaaa agttcaaaat tgttatttgt gtggtacctg tactgcaggt tgtcctgtaa
     4141 gtagtttata tgacgactat aaccccagac gttttatgcg gatgatatta ctgggtatga
     4201 aggaggatgt actctcttct cctgaaatat ggcagtgcag ccaatgccat atttgtgttg
     4261 cgcattgtcc tcaagatgtg agatttgcag atatagtaag ggttttaaga aatatggcag
     4321 ttgaagaagg ttatattccg gaaagcatat tgaaggaaat tgaaagtatt gatattgatt
     4381 taaaaaaaca gcgaatcatg aaagttaaag aattaacatc tgaaaaaatg cataagcaga
     4441 aataaatttg gaggatatta tgagaaaacc cgtacttgta ttaggaggag gaatagcagg
     4501 aatacaggct tctcttgatt tagcagaaat gggtatccct gttttcatag ttgagaaaac
     4561 accaagcatt ggcggaagaa tggcgcagct tgacaagact tttcccacca atgattgttc
     4621 tgcttgcatt cttgccccta aagttacttc atgttttaat caccctctga taaaaacatt
     4681 tacatggagt gaggttctgg atataaaagg tgaagcgcct gactttactg cggtaataaa
     4741 gaaaaaggcc aggtttatag atgcgaataa atgtacagga tgtggagaat gtacacttaa
     4801 gtgtcctata gaagccaaaa acgagtttga tatgggaatt gggaaaagac atgcaattta
     4861 caaaccgttc gctcaggctg taccaaacaa agttgcaata gataaaaaag gaacatcccc
     4921 atgtaagttt aattgtcctg cacatatcga tgctcatggc tatgtagctc ttattggcca
     4981 agggagatac aaagaggctc tggaacttgt aaggaagact acaccctttg caggtgtttt
     5041 aggcagagta tgttttcacc cttgtgagaa tgcctgcagc aggcaatatt tagattctcc
     5101 catatcaata gcaggattga aacgatttat tgcggattat gaagtatcac agggaataaa
     5161 accacaggta agattaaata ccggattaaa tacaaacacc ggagtaaaca ctggagtaaa
     5221 cactggaata aatgctgatt tgaatacttc agagaatgca aagaagaaag ttgctgtaat
     5281 aggttccgga cctgcaggac ttaactgtgc ttatcaatta acacttatgg gttataaggt
     5341 ttcgatattt gaagcattgg aagaaccagg cggaatgctt aaagtaggaa ttcccgacta
     5401 tagact
//
LOCUS       DAOSDD010000273         5400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5405_length_5400_cov_4.631618, whole genome shotgun sequence.
ACCESSION   DAOSDD010000273 DAOSDD010000000
VERSION     DAOSDD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5405_length_5400_cov_4.631618"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..799
                     /locus_tag="PK733_17250"
     CDS             257..799
                     /locus_tag="PK733_17250"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ12314.1"
                     /translation="MIYEEKIIERKKMFKGNIIDVEKYTVELPNGKITTRDVVVHPGA
                     AVIIPISQKGEVYLVKQYRTPIEKVLLELPAGKLDPGEAPEICAARELKEETGLNADC
                     IKHVISIHSTPGFCNEVLHVFMATGLHEGNSCADEDEFISSVKIHIGKLIEMIFNHEI
                     TDAKSIIGIMIAERIINKEV"
     gene            972..1613
                     /gene="spoIIM"
                     /locus_tag="PK733_17255"
     CDS             972..1613
                     /gene="spoIIM"
                     /locus_tag="PK733_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOJ12315.1"
                     /translation="MINKTRELLGRHIKENSNRYFLLFMAFIIGVSAGAFTVNGLSTL
                     QTEELIHYFQGFIQLMEKQRLNSNEILMISLQDNAKIVILLWVLGLTIIGIPFIILLI
                     IIRGFITGFSSGFIIKILGLKGALFNLFAILPKEFVIIPCIIALGVNGINFSLSITKS
                     KSIKQILKENLKMNFIGYSMTTIFFSILILLGILVEAYIVPVFIRIITPIMTT"
     gene            1730..2614
                     /gene="xerD"
                     /locus_tag="PK733_17260"
     CDS             1730..2614
                     /gene="xerD"
                     /locus_tag="PK733_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOJ12316.1"
                     /translation="MKELVQKYIDYLENGRQLANNTLQSYKRDLEQYISYLNEMNLCN
                     INATDKTTVINYLLHLQKKGRATSTISRNLASIRSFYQYLAKSNIIDTDPTLELESPK
                     VEKKLPQILSPQEVELLLEQPKCIDLKGYRDKAMLELLYATGIRVSELINLDCSDINY
                     DLSYIKCNNGVKERVIPIGSLAIKALQDYLLKARKNMIRNNDEKALFININGKRLTRQ
                     GFWKIIKQYKNQAKIGKDITPHTLRHSFAAHLLENGADLRSIQEMLGHSDISSTQIYA
                     QIARNRIKEVYKKTHPRA"
     gene            2756..3919
                     /locus_tag="PK733_17265"
     CDS             2756..3919
                     /locus_tag="PK733_17265"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOJ12317.1"
                     /translation="MKRAIIIVLDGVGIGELPDAEKYGDKGSNTLGNIVKTINGFRLP
                     NLEKLGLSNIAGMIGFPENNHPEACFGRMKEKSPGKDTTTGHWEIAGIVMDKPFPVYP
                     EGFPKTIIHEFEKAINRKVLGNMAASGTEIIKTLGHQHVITGYPIVYTSADSVFQIAA
                     HEDVIPVDRLYEMCRIAREILKGEHSVGRVIARPFTGTEGKYARTERRRDFSLKPIKK
                     TLLDYAVEKGYQVKAVGKIEDIFANQGITESVHTHNNTAGIRKTIDFIKEDFSGLIFT
                     NLVDYDMLYGHRNNVEGFALALKEFDEKLTEILNNIKKNDIIFVTADHGCDPTTPSTD
                     HSREYVPLIVYGNNINKGVNLGTRESFADLGATIAEYLGTGYTGEGISFLGQL"
     gene            4027..5355
                     /locus_tag="PK733_17270"
     CDS             4027..5355
                     /locus_tag="PK733_17270"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOJ12318.1"
                     /translation="MRMYNIIEKKRDGCELSYEEISFFIDGVIKENIPDYQTAALLMA
                     IFIRGINPRETADLTRLMVDSGDKIDLSSIKGIKVDKHSTGGVGDKTTLIAAPVVAAC
                     GIPVIKMSGRGLGHTGGTIDKLQSIPGFKTDIDKEEIIKNVKRIGISIAGQSNNLVPA
                     DKKLYALRDVTATVDSIPLIAASIMSKKIASGTDKIVLDVKAGSGAFMKDIDHAISLG
                     KTMVEIGERTSRETVVIVTDMDIPLGNAIGNSLEVIEAIEVLKGRGPSDLKTISLEIA
                     AHMLKLAGMGNMEECRVKARNAIDSRKAMEKFKELVKAQYGIDDVIEDYSIFKQPLYK
                     YDIAADMEGFIESMDTRLIGQVSSILGAGRQTKESLIDYSAGVYIYKKTGMEVKKGEI
                     IATLYTDKSEIADEAVSLLKGAFNYSRNTPQLRPLILAYVDSKHTFRYAD"
BASE COUNT         1974 a          750 c         1113 g         1563 t
ORIGIN      
        1 caattataga ggctatggaa gaatgtacga agaaggccag aaaaattgga gagacatata
       61 ataaataatc taacttgcag acttcatttt gagcttgtaa attgtgcagt tgctgaataa
      121 aaagattggg cagaatagtg cactatccaa gaatagcaga atagtagaat agcagaatag
      181 tagaatagta ggatggttga atagtagaca gttgaatggt tcaatagttg aacagttggt
      241 gataggagaa cagtgtatga tttatgagga aaaaattata gaaagaaaga aaatgtttaa
      301 gggcaatatt attgatgtgg aaaaatatac agttgagctt cccaatggga aaataactac
      361 gcgggatgtg gtagtacatc ctggtgctgc cgtaataata ccaattagcc aaaaaggaga
      421 ggtttatctg gtaaagcaat ataggacacc aatagaaaag gtattgctgg agcttcctgc
      481 cggaaaactt gatccaggag aagcgcccga aatctgcgct gcaagagagc ttaaagagga
      541 aaccgggtta aatgcagact gtataaagca cgttattagt atacatagta ccccaggatt
      601 ttgcaatgaa gtacttcatg tttttatggc aacaggtctt catgagggaa attcctgtgc
      661 tgatgaagac gaatttattt cttctgtaaa aatccatata ggcaaactta ttgaaatgat
      721 ttttaaccat gaaataactg atgctaaatc aataattggg ataatgattg ctgagaggat
      781 tataaacaag gaagtttgac taattatgtt agcttaatta ttaattaagt agaatgcagc
      841 aattcttgtg actgctcctt tcatttactt atatcttgaa aaaggtttac aatatatgtg
      901 cccatagtgt aattttttga ctttatcctg cataaacatc ttagtgataa aggaaataag
      961 aaggggtaca tatgattaat aaaacaagag aattactggg caggcatata aaggaaaatt
     1021 caaacagata ctttttacta tttatggcat ttattattgg agtttcagca ggagccttta
     1081 ctgtaaatgg tttaagtaca ctgcagacag aggagttgat tcattatttc cagggcttta
     1141 tacagttaat ggagaagcaa agacttaaca gtaatgaaat attaatgata tctttgcagg
     1201 ataacgcaaa aattgtaatt ctattgtggg tattaggttt aactataata gggattcctt
     1261 tcattattct ccttattatt ataagaggtt ttattacagg attcagctct ggatttatta
     1321 ttaaaatatt aggtttaaaa ggtgctttat tcaatttgtt tgcaatattg cctaaggagt
     1381 ttgttattat accatgtatt attgctttag gtgtaaatgg tattaatttt tcactaagca
     1441 taacaaaaag caaatcaata aagcagattt taaaagagaa tttaaaaatg aatttcatcg
     1501 gatatagtat gactactata tttttcagta tattgatatt acttggaata ttggtagaag
     1561 cgtacatagt acccgttttt atacgaatta ttacaccaat tatgactaca taatgtataa
     1621 atgtggtaaa tcagggcaat aattattaga gtagattgac cgggtagaac taccttgtaa
     1681 tcaataaata gtatatagtt caaaaatatg aaagaaggag tagtttttaa tgaaagagtt
     1741 agttcaaaaa tatattgatt accttgaaaa cggaaggcaa ttagccaata atacacttca
     1801 atcttataaa agggatctgg aacagtacat atcatatctg aatgagatga atttgtgcaa
     1861 tataaacgct accgataaaa ctacagttat taattattta ctgcatttgc agaaaaaagg
     1921 cagggcaact tcaaccattt caagaaatct tgcatcaatc agatcttttt atcagtatct
     1981 ggctaaaagc aacataatag acacagaccc tacattggaa ctagaatctc caaaggttga
     2041 aaaaaagctt ccacaaattc tctctcctca ggaagtggag ctgcttcttg aacagccaaa
     2101 gtgcattgat ctaaaagggt acagagacaa ggcaatgctt gagttattat atgcaacagg
     2161 aattagagta tcggaattaa taaaccttga ttgctccgat attaattatg atttgagtta
     2221 tattaaatgc aataatggag taaaagaacg tgtaattcca atcggttcac ttgcaataaa
     2281 agcattgcag gattatttac tcaaggcaag aaaaaatatg atcaggaata atgatgaaaa
     2341 ggctctgttt ataaacataa atggtaaaag gcttacaagg cagggtttct ggaaaataat
     2401 caagcagtat aagaatcagg ctaaaattgg taaagacatt acaccccata cattaagaca
     2461 ttctttcgca gctcatctgc ttgaaaatgg ggcggattta agatcaatac aggagatgct
     2521 tggtcattcc gatatatcat ctacgcaaat ttatgcacaa attgcaagaa atagaattaa
     2581 ggaagtttac aaaaagacac atccgagagc ataatatatg aagagcagct tatgatgtaa
     2641 taaacatgaa gtactactta tgatataaca aacacgttaa ggggacatat atcctgacta
     2701 tatgggtatg gcccctttta taatatactt gatattactc agaaaggaaa tatttatgaa
     2761 acgagcgatt attattgtat tggacggagt tggaataggc gaactgcctg atgctgaaaa
     2821 gtatggtgat aaaggcagta atacccttgg gaacattgtt aaaacaatta atggcttcag
     2881 acttcctaat cttgaaaaac tcggtctttc aaatattgcc ggcatgattg gcttccctga
     2941 aaacaaccat ccggaagcct gtttcggacg tatgaaagaa aagtcaccag gtaaggatac
     3001 gacaacagga cattgggaga ttgcaggtat tgttatggac aagccattcc cagtttatcc
     3061 cgaagggttt ccaaaaacca taattcatga gtttgaaaag gctataaata gaaaagtcct
     3121 tggtaatatg gctgcatcag gtacggaaat tattaagacg ttaggacatc aacacgtaat
     3181 tactggttat cctattgtat atacatcagc agatagtgtt tttcagattg ctgcccatga
     3241 agatgttata cctgtagata ggctatatga aatgtgcagg atagccaggg aaattttaaa
     3301 gggggaacac tctgtaggca gggttatcgc cagacctttt acaggcacag aaggtaaata
     3361 tgcccgtact gaaaggaggc gtgatttttc tttaaaaccc ataaagaaaa cactgttgga
     3421 ttatgcagta gaaaaaggct atcaggtaaa ggctgtaggt aaaattgagg atatctttgc
     3481 taatcaggga attaccgaat cggtgcatac tcataataat acagctggaa tacgcaaaac
     3541 tatagatttt attaaagaag atttttcagg attaattttt acaaatcttg ttgactatga
     3601 tatgctttat gggcatagaa ataatgtaga ggggtttgca ttagcattaa aagagtttga
     3661 tgagaaactg acagaaatat tgaataatat aaagaaaaat gatattatat ttgtaaccgc
     3721 agatcatggc tgtgacccta caaccccaag tacggatcat tcaagggagt atgtaccgtt
     3781 aatagtctac ggaaataaca taaataaagg tgtcaatctt ggtacaagag aatccttcgc
     3841 agatctcgga gctacaatag cagaatatct tggtacaggg tatacaggtg agggaattag
     3901 tttcttaggt caattgtaac taatttgtaa ctgatttagc cttatgtttc aaaacacatg
     3961 ggtttcattt aatattttat ctctacagtg ttttggaaaa tataagataa aagaggatta
     4021 cttaaaatga gaatgtataa tataattgaa aaaaaaagag atgggtgtga actttcatat
     4081 gaagaaatta gcttcttcat agatggagtc attaaggaaa atattcctga ttatcagaca
     4141 gcggccttgt taatggctat atttatacgt ggcataaatc caagggaaac tgccgattta
     4201 acgagattaa tggttgattc aggggacaaa attgacttat cttcaataaa aggtattaaa
     4261 gttgataagc acagcacagg aggggtaggg gacaaaacta cattaatagc agctccggtt
     4321 gttgcagcct gtggtatacc tgtgataaaa atgtcgggaa gggggttggg acatacaggc
     4381 ggtactatag ataaacttca atcgattcct ggatttaaga ctgacattga taaagaggaa
     4441 atcataaaaa atgtaaaaag gattggtata tctatagcag gtcaaagcaa taatctggta
     4501 cctgcagaca aaaagttata cgctctaagg gatgtaactg ccacagttga cagtattccg
     4561 ctaattgcag caagcattat gagtaaaaaa attgcatcag gaacagataa aattgtactg
     4621 gatgtaaaag cgggaagcgg agcttttatg aaggatattg accatgctat ttctcttggt
     4681 aaaaccatgg tggaaatagg tgaaagaaca agccgggaaa cagttgtaat tgtaactgat
     4741 atggacattc ctcttggaaa tgctatagga aattccctgg aagttataga agcaatagaa
     4801 gtgttaaagg gaaggggacc ttctgattta aaaacaatat ctcttgaaat tgcagcacat
     4861 atgctgaaac ttgccggtat gggaaatatg gaagaatgca gagtaaaggc tagaaatgca
     4921 attgatagca gaaaagccat ggaaaaattc aaagaattag taaaagcgca gtatggaatt
     4981 gatgatgtta ttgaagatta cagtatattt aagcagccat tgtacaaata cgatatagcg
     5041 gcagatatgg aaggcttcat tgaatctatg gacactaggt taataggaca ggtatcatca
     5101 attttaggtg ccggcaggca aacaaaagaa agcctgattg attattcggc cggtgtatat
     5161 atttataaaa aaacaggtat ggaagtaaaa aaaggtgaga taattgcgac cctgtataca
     5221 gataaaagtg aaattgctga tgaagctgtg agtttgttaa aaggtgcttt taactactcc
     5281 cgtaatacgc cacaattaag accattgata cttgcttatg tagatagtaa gcacacattc
     5341 agatacgctg attaataaaa tacaaaatat aaaatattac tgcataatac agaatataca
//
LOCUS       DAOSDD010000274         5329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5491_length_5329_cov_4.245734, whole genome shotgun sequence.
ACCESSION   DAOSDD010000274 DAOSDD010000000
VERSION     DAOSDD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_5329_cov_4.245734"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="proC"
                     /locus_tag="PK733_17275"
     CDS             complement(<1..833)
                     /gene="proC"
                     /locus_tag="PK733_17275"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ12319.1"
                     /translation="MSMNMNTRIGFIGAGKMGSAIAKGISGSGKVFPGNIHIYDIDSD
                     KTALLHNETGVSIEKSSIEVIEKCDIIVLAVVPGIIKSVLEPCSHLFDNSKIFVSIAA
                     GVPIKVYKEILGDNAKIVRTMPNRPALIGRGMTLISFEKNSLSEIELYTIKDLFECVG
                     KVEVLDESLMDEVIALTSSSPAYVFMLIEAMANAAVLMGIPAKSAYTMAAQAVSGSAE
                     MVLETGRHPAELKDEICTPAGTTIEAVKALEKNGFRYAIIEAMEECTKKARKIGEKYN
                     K"
     gene            complement(921..998)
                     /locus_tag="PK733_17280"
     tRNA            complement(921..998)
                     /locus_tag="PK733_17280"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(961..963),aa:Pro,seq:ggg)
     gene            complement(1107..1493)
                     /locus_tag="PK733_17285"
     CDS             complement(1107..1493)
                     /locus_tag="PK733_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12320.1"
                     /translation="MPGIIYIARIYAGIDNLKASIVLEKATEKGKHIQEQYHKDNNLG
                     SIVKIFSENGYFEIITLFKIADKYIELLEKDKAAGVLAKSLEVIELINEKRTRASYLA
                     DIDVKYIQAGIKPGVLKKEMQYKILN"
     gene            complement(1447..1710)
                     /locus_tag="PK733_17290"
     CDS             complement(1447..1710)
                     /locus_tag="PK733_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12321.1"
                     /translation="MKKILYCIIICIILSVLTGCLTINNMQNRRFAAADKSDMTDKSH
                     VAVISDTAPIPSKVEMDIFRLSLLLADEHAGNYIYSQDICGYR"
     gene            2199..2795
                     /locus_tag="PK733_17295"
     CDS             2199..2795
                     /locus_tag="PK733_17295"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOJ12322.1"
                     /translation="MLKNSYLNMVNEKETINDELSKLKKQNEDLLLKNNELLTENDKL
                     DNKNRDIMEDNIALQNSLKLAASVGIKPQNYTAFNGINSRGSIDRGNYIGKFLGTAYT
                     PSVEECGNNKGITNSGKPIIPGVSVAIDKNYWPFGTVFYIKGLGYAVAMDTGNGVKGK
                     YRFDFAVFDKEFAKVIGSRKWDVYLVRMGTGKVEDIKF"
     gene            complement(3272..3883)
                     /locus_tag="PK733_17300"
     CDS             complement(3272..3883)
                     /locus_tag="PK733_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12323.1"
                     /translation="MMASRLMHYIISHEIMKETSFDKDLFLLGNLAPDAHDGSLYGNS
                     HAHFRKIIGNEYDSYPNIDLERFKEQYLNKKNNIDDFIIGYYCHLVSDANWVNSIYPK
                     YLKYDLDIDEARYQRELLFNDLRIFNTLLREYFNVDFQNDIILPVKFGISEFSHNKLI
                     LLLNALSNDMTDNYDGNLEIFNFEFILDYIKVTISKCINELRP"
     gene            complement(4219..4632)
                     /locus_tag="PK733_17305"
     CDS             complement(4219..4632)
                     /locus_tag="PK733_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ12324.1"
                     /translation="MNNKIIVVVKGVILYNGKVLIIRRDDNDDVGAGTWECVGGKIDF
                     GEGLEQALKREVCEEVGISIDVDKLLYATTFKTDPLRQIVILTYKCIATQNNVILSKE
                     HSDYKWVAENDFRELLHKPIIADMEKHNAIKSIFE"
     gene            complement(4821..4976)
                     /locus_tag="PK733_17310"
     CDS             complement(4821..4976)
                     /locus_tag="PK733_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12325.1"
                     /translation="MDNDLYTNIGYFVVLCMKYIFIPIVVGVAIRVISRKLLQPQPEK
                     QKKKTVF"
BASE COUNT         1649 a         1056 c          848 g         1776 t
ORIGIN      
        1 tatttattat atttctctcc aatttttctg gccttcttcg tacattcttc catagcctct
       61 ataattgcgt atctaaaacc attcttttcg agtgctttca cagcttcaat tgtagtccct
      121 gctggcgtac atatttcatc tttaagttcc gccggatgcc ttcctgtctc cagcaccatt
      181 tcagctgaac ctgatactgc ctgtgctgcc atggtatagg cggatttggc gggaataccc
      241 attaatactg cagcattagc cattgcttca attaacataa atacataagc agggctgctt
      301 gaagtaagag ctattacctc atccataagg gattcgtcta atacttcaac ctttcctacg
      361 cattcaaata aatcttttat agtataaagc tcgatttcac ttaaactatt tttttcaaag
      421 gagatcaatg tcatgcctct gcctattaat gcaggcctat taggcattgt tcgtacaatt
      481 tttgcattgt ctcccaatat ctctttataa acttttattg gaactcctgc tgcaatagat
      541 acaaaaattt tactattatc aaaaagatgg ctgcatggtt caagaacaga ttttattatg
      601 cccggaacaa cagcaagaac tataatatcg catttttcaa ttacttcaat actgcttttt
      661 tcaatactaa ccccggtttc attatgtaat aaggctgttt tatccgaatc aatatcatat
      721 atatgaatat tacctggaaa tacttttccc gaacctgata tacctttcgc tatagctgag
      781 cccattttcc cggcaccaat aaaaccaata cgcgtattca tattcatact catagcaaca
      841 taccctccaa acacaacaat ttacatatcc tatatgaaat taagcggcta cagtctataa
      901 atcctgtaag ccgcttattt tggtcggagt gactggactt gaaccagcga cctcttgcac
      961 cccaagcaag cactctagcc aaactgagct acaccccgtt cacgattaga aattataaca
     1021 catgggttta caaaagtaaa ggggttttcc atttttattt tattgctata aattaccata
     1081 aactacaaaa ttataattta tgaaaatcaa tttagaattt tatactgcat ttcttttttc
     1141 aacacccctg gtttaatacc tgcttgaata tatttcacat cgatatctgc cagatatgat
     1201 gctcttgtac gtttttcatt tatcagctca attacctcaa gagatttcgc aagcacaccg
     1261 gctgctttat ccttctcgag cagttcgatg tatttatcag caattttaaa caacgttatt
     1321 atttcgaaat acccattttc cgaaaatatt tttacgatgc ttcctaaatt attatcttta
     1381 tggtattgct cctgaatatg ctttcctttt tcagttgctt tttccagaac aatagatgcc
     1441 ttaaggttat ctatacccgc atatatcctg gctatatata taattcccgg catgctcatc
     1501 agctagtaaa aggcttaagc ggaatatatc catttctact ttcgaaggta taggggcagt
     1561 atcagaaata acagctacat gagatttatc cgtcatatca gacttatcag cggcagcaaa
     1621 tcttcggttc tgcatattat tgattgttaa gcacccagta agcactgata atattatgca
     1681 tatgattata caatatagta tttttttcat ataaaccaac tttctattac tcacattata
     1741 taaaaagcat ttctgtgaaa aatatttagt atgacttggc aatgtggaat tattgcataa
     1801 tttgttaaag gtttctatta tttatgacgg tattatacct tgttttgttc caaaaaattt
     1861 tgtttttatg ttgctttgta taaaaatgca aaaggatgga tattctaacg atgtgtattt
     1921 taattttata taaaaaaatc atttaacata tatatatttg cattattaac aaacaatagg
     1981 aggatgattt aatcataagg aattttaata gaaatttcgg tataatactg atattattcg
     2041 ttataatttc atttttatgc ggaaataacc tggctgtata tagttcatac agacaggcat
     2101 ataaggaact tgagtctctt actataaaaa caaaagaatc ttctataatt aacgagcggt
     2161 taagttctac acttaagcaa ctggaagaag agaatgactt gcttaaaaat agctatttaa
     2221 atatggtaaa tgaaaaagaa actattaatg atgaattatc aaagcttaaa aaacaaaatg
     2281 aagatttgtt attaaaaaat aatgagctgc ttaccgagaa cgataaattg gataataaaa
     2341 atagggacat tatggaagat aatattgctc ttcagaactc tcttaagctt gcagcttctg
     2401 ttggaataaa acctcaaaat tatactgctt ttaatggtat aaattcaaga ggcagtattg
     2461 acaggggaaa ttatattgga aaattcttag gtactgcata taccccaagt gtagaagagt
     2521 gcggtaacaa taaggggatt actaactccg gcaaacctat aatacctgga gtatctgtcg
     2581 caattgataa gaactactgg ccttttggga ctgttttcta tattaaggga ttagggtacg
     2641 cagtcgccat ggatacgggt aatggagtaa aaggtaaata caggtttgat tttgctgttt
     2701 ttgataaaga atttgctaaa gtcataggct caaggaaatg ggatgtatat cttgtaagaa
     2761 tgggaactgg taaagttgag gacataaaat tttaataaac aaaagaaccg tcccctcgtt
     2821 cctccctcgt tcctactgga aatatacccg ctaacattgt ttaaatccgt actacctgta
     2881 taccgatctc ttgccgcaaa cttcaaattg cgtacatttg ccatgattgg tgcaccaaac
     2941 ataatagaca aggttctgta gcagtataca gtatataatt ccgaatgttt ggatggttaa
     3001 atttaatata caaatctaca ataaccttct cttcacccga cgactggaag cccgtgtgac
     3061 attattgtgc catatttcag agaacgtccc gcagtcgcga cgacgatgag ctgtgaccct
     3121 cgaagccctt ctccctggcg gtgcttgcca cccagcgaat cggtgtggcg tgctgcaggg
     3181 actatcattt cggggactca gaccatgcac gaccttgcgt tgactgcttt gttatgtaaa
     3241 gtaccgccca ccttccggtc gctcgacgga gtcaaggccg gagttcatta atgcatttac
     3301 ttatagttac ttttatataa tcaagaataa actcaaagtt aaatatctct aaatttccat
     3361 cataattatc agtcatatca ttgctaagtg cattaagtaa aaggataagt ttattatgcg
     3421 aaaactcact tataccaaat ttaactggta atattatatc attttggaaa tcaacattaa
     3481 aatattcacg caacagtgta ttgaatatcc tcaaatcatt aaatagtaat tctctttgat
     3541 atcgagcctc atcaatatcg agatcatatt tcaaatactt gggatatata gaattgaccc
     3601 aattagcatc agatacaagg tgacaataat atccaattat aaagtcatct atattattct
     3661 ttttattaag gtattgttcc ttaaagcgtt ctaaatctat gttagggtag ctatcatatt
     3721 catttccaat gatctttcta aaatgagcgt gcgaattccc gtaaagtgat ccgtcatgtg
     3781 catctggagc cagattacct aacaaaaaca aatctttatc aaatgaagtc tccttcatta
     3841 tttcatgtga aattatatag tgcatgagtc ttgaagccat cataatcact ctctttcata
     3901 ataatgtatc gcagagtgtt ttattttcgc ccggcggccg gaggactggg cgacgtagat
     3961 gaaccgcatt acgtataacg tctggtttgc ccgacgccct caaagcctac aaaggaaggg
     4021 gctcatacct tggtaccttc tgggtgggct aataccccga ctggctcggt tggctttgag
     4081 ggtgtgccgt gcaaactccg gagcctttcc cttcacggct ttacaggcaa acattgttag
     4141 gtgctgtttc acgacctacg aagcaagagc ggcacggatg ccgcggcctg aagtcttgac
     4201 ctcatcaaaa ttactctact attcaaatat actttttatg gcattatgct tttccatatc
     4261 agcaataatt ggtttatgta ataattctcg aaagtcattt tctgcaaccc atttataatc
     4321 actatgttcc tttgatagga taacattatt ctgggtagct atgcatttat atgtcaaaat
     4381 tacgatttgt cttaatgggt cagttttaaa agttgttgca taaagcaact tatctacatc
     4441 tatactaatt ccaacctctt cacaaacctc cctttttaat gcttgttcga gtccctcacc
     4501 aaaatcaatt ttacctccaa cgcattccca tgtacctgca cctacatcat cattgtcatc
     4561 tcttctaatg attagaacct taccgttata taaaattact ccttttacta caacaataat
     4621 tttattattc ataataaatc tccaatacat tgcaaccata tatcataata actcatcata
     4681 ttcgtttcat ttgtagtatg ttttaccatt ttattgaaat tactgtttac attaaaccta
     4741 tttgtgctat actataaata aggacggcga cgcttgttgc tgtcaggtta taattgataa
     4801 attcttattt tgaacggttt ttaaaagacc gtttttttct tttgcttttc aggctgtggt
     4861 tgaagcagtt ttctggaaat tactctaata gctaccccta ctacgatagg tatgaatatg
     4921 tatttcatac acaagactac aaaatagcct atattcgtgt ataaatcatt atccacatcg
     4981 accacctcct tcctttgtgg attccctgca gcaactataa cgtcgccatc ctcatgtgtg
     5041 ggaatacaaa ggaaggaacc ttaaagctaa tcactcatct caaacctgat tgtttatcac
     5101 ctgtccattg taccatatgc aattaatatt tgcaatatat tccagaacat ttgtttatcc
     5161 ttatttaatg ctgttctatg attttctaca aagaaccacc aaagaacacc cgcgacacga
     5221 tgtcgcagca atacccagtc tttcatgtaa cgactcgata gcaataggta aggttttgag
     5281 gtaatctcca ccttgtcccc tgctccaaac cggataggaa cctttcaat
//
LOCUS       DAOSDD010000275         5304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5522_length_5304_cov_3.375310, whole genome shotgun sequence.
ACCESSION   DAOSDD010000275 DAOSDD010000000
VERSION     DAOSDD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5522_length_5304_cov_3.375310"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK733_17315"
     CDS             <1..458
                     /locus_tag="PK733_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12326.1"
                     /translation="IPSLYSVWNIVIMRNYISTIPEEMEEAAVVDGAHPLYIIFMIML
                     PLSLPIVATVSLWNAVGHWNAWFDAMIYITDGKKQVLQMILRKIVIMEEMRHLIPEMD
                     AALTSQGVQYTELTVKAATIMLTIGPIIAAYPFAQKYFVKGILIGSLKG"
     gene            549..2105
                     /locus_tag="PK733_17320"
     CDS             549..2105
                     /locus_tag="PK733_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12327.1"
                     /translation="MKKLLAIIIVLVMLLTIISGCSGGKKSDNKNDSINQTEQAAKEP
                     TKILWAAGGSGSNIPSKDGIVVKALEEKFNVDISYFGSTFVDNKEQFTIKIGSGEIPD
                     VWYDWNTDTWWEQGILRTVMEELIKQYMPKTYGDIFLKLDPKKMAFSVRKTEDGDLKG
                     IPQLSLAGASYFIRVYRQDWLDKFNLKVPTTVEELENVIKVFTKDDPDGNDKNDTYGV
                     NVQSNMVGFSDVFGTFGIAPGIWVLKNDKVVFSDTTNEYKEALKILARWYADGYINPE
                     CLTDSFETFTSKFADGKIGSYYQNWYWASSDQTNTPVSMLLKKDLNAKVVYGDAIKGP
                     NGEAGSYGFGPDLGWTVQFRKGLSDEVVIKVMQIIEYLSTDIEGFKLHHGGIEDKTYI
                     IEDGMIKNNLLPEEKIKEGIDVFPFFTGEKFKDLYWGTGESGKLFDLGTKQPWITNVI
                     GRSPLVKAREKGIGTDIGKVREEFYWNVITGKVNADTEWDAYQKRLNDNGLQEMTEEA
                     NEIYDSVYRK"
     gene            2243..3793
                     /locus_tag="PK733_17325"
     CDS             2243..3793
                     /locus_tag="PK733_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12328.1"
                     /translation="MSSIMLNEDCNHFIHTRHVAGIQVGINELCSFIDQYANTDVKDL
                     LFNVNAMLAFFPSKSRENAIDKYLRLLKAGKDMDISGTAVKTAYDIFVKQNLDMYKIW
                     IERSRYNGISPWISIRMNDIHNNQDEGHFLLSSFFEENKHMRRVTHRPNSNYFDNALD
                     YTFPEVQEHYLSLIKEVVERYDFDGLELDWMREVYLFKIGGESEWLKILTNFMRIVRK
                     ILDQAEKERGHKIKICVRVPYSPEVALRFGLDVLTWARNGLVDVIVPTARWATTDNNM
                     PIDFWKQILDGTGAIVAAGIEILLEATRANPKYNAFNSLETARGSAYTYLNMGADKIY
                     LFNYMDQPSLLSHSLEFNSAVDKRNYHELLITICDLETASKLPRRNVVTYHDIAAPGL
                     RNGDLLPLCCSSPNSNGSMIFKNIRIETGPINKNLKRSLVLGIDSKRELDSSDFDVYW
                     NTNKCKLVGIITLSAPKPNCKTYKFKIECSEKSQEAFPSASIIEIASKNIPFSVEWAE
                     ILINYQGC"
BASE COUNT         1820 a          793 c         1064 g         1627 t
ORIGIN      
        1 ttataccaag cctttatagt gtttggaata tagtaattat gaggaattat atttctacta
       61 ttcctgaaga aatggaagag gcagcagtgg tggatggtgc acatccttta tacataatat
      121 ttatgataat gctcccccta tctcttccca ttgttgccac tgtttctctt tggaatgcag
      181 tgggacattg gaatgcttgg tttgatgcta tgatttatat tactgatggc aaaaaacagg
      241 tgttacaaat gatccttaga aaaatagtaa ttatggaaga gatgcggcac cttattccag
      301 aaatggatgc tgctttgaca agtcaaggtg ttcaatatac agaactaaca gtaaaggctg
      361 ctaccataat gcttaccata ggaccgataa ttgctgcata tccctttgca cagaaatatt
      421 ttgttaaagg aatattaatt ggttctctga agggataggt tgcttagttt taaagcattg
      481 tagaattaaa tggttaatgc aatgctttaa tataaaaaac attaaaaaat aacaatagga
      541 ggtatattat gaaaaaatta ctagctatta ttattgtatt ggttatgctg cttaccatta
      601 tatccggctg tagcggaggt aaaaaaagcg acaacaaaaa tgacagtata aatcagacag
      661 aacaagctgc aaaagagcct acaaagattc tttgggctgc cggtggctcg ggctcgaaca
      721 ttccatctaa agacgggatt gttgtaaaag ctttagaaga aaaattcaat gtagatattt
      781 cttattttgg cagtactttt gtcgacaata aagaacaatt tactattaag attggctccg
      841 gtgagattcc tgatgtttgg tatgactgga acaccgatac atggtgggaa cagggtatac
      901 tacgaactgt tatggaagaa ctgatcaaac agtatatgcc taaaacttat ggcgatattt
      961 ttttaaagct tgaccccaaa aaaatggctt ttagtgttcg taaaacggaa gacggagacc
     1021 tgaaaggtat accacagctt tcattggcag gtgcttcata tttcataaga gtctacagac
     1081 aggactggct ggataaattt aatcttaaag ttccaacaac agtggaagaa ctggaaaatg
     1141 taataaaggt attcactaaa gatgatccag atggaaatga taagaacgac acttatggtg
     1201 taaatgttca atctaacatg gtaggattta gtgatgtttt tggaacattc ggcatagcac
     1261 ctggtatatg ggtactgaaa aatgacaaag ttgttttttc tgatacaact aatgaataca
     1321 aggaagcctt aaaaatttta gctaggtggt atgcggatgg gtacatcaac cccgaatgtc
     1381 tgacagattc atttgaaacc tttacttcaa aatttgctga tggaaagata gggagttatt
     1441 atcagaattg gtattgggct tcttcggacc agacaaacac accagtcagc atgttgctta
     1501 aaaaagacct aaatgccaaa gttgtatatg gagatgctat aaaaggacct aacggggaag
     1561 cggggtcata cggttttggt ccggatttag gttggacagt acagtttcgt aagggtctaa
     1621 gtgacgaagt agtaataaag gtaatgcaga ttattgaata cctcagtaca gatatagaag
     1681 ggtttaagtt gcaccatggt ggtattgaag ataaaactta tattattgaa gatggtatga
     1741 taaaaaataa tcttttacct gaagaaaaaa ttaaagaagg aatagatgtc ttcccatttt
     1801 ttacaggtga aaaatttaaa gatttatact ggggaaccgg cgaatcaggt aagctatttg
     1861 atttaggtac aaaacaacct tggataacta atgttatagg taggtcaccc cttgtaaaag
     1921 cccgtgaaaa gggtattggc actgatatag gtaaagtacg agaagagttt tactggaatg
     1981 taattaccgg caaagtaaat gctgatacag aatgggatgc ctatcaaaag aggttgaatg
     2041 acaatgggct tcaagagatg acagaggaag caaatgaaat atatgatagt gtttatagaa
     2101 aatgaaggaa agaatatata gatattatga ataatcctat tgataaataa tagggcttaa
     2161 tatttcctta atataagcag ggaaatgcct tatggcaatt tccctattta tttaacagac
     2221 tgggtgtaaa ggaggaatga cgatgagtag cataatgcta aatgaagatt gcaatcactt
     2281 tattcataca aggcatgtgg cgggcataca ggtagggata aatgaattat gtagttttat
     2341 tgatcaatat gctaacacgg atgtaaaaga tttgctattt aatgtcaatg ctatgcttgc
     2401 ctttttccct tcaaaatcac gtgagaatgc aatagataaa tatttgaggt tacttaaagc
     2461 cggcaaagac atggatataa gcggaactgc cgttaaaact gcatatgata tctttgtaaa
     2521 gcagaatctt gatatgtata agatatggat agagcgctca agatataatg gaatatcccc
     2581 ttggatatca attcgtatga atgatatcca taataatcag gatgaaggtc attttctgct
     2641 atcatccttt tttgaagaaa ataagcatat gcgtagagtt actcaccgcc ctaattcaaa
     2701 ttattttgat aatgcactgg attatacatt ccctgaggtt caagagcatt atctttcttt
     2761 gataaaagaa gtggttgaaa gatatgactt tgatggactt gaactggatt ggatgcggga
     2821 agtgtatctt tttaaaatag gtggggaatc tgagtggcta aaaattctta ctaattttat
     2881 gcgtattgta cgaaaaattt tagatcaggc ggagaaagaa aggggtcata agattaaaat
     2941 ctgtgtacga gtgccctata gcccagaggt agcattacgt tttggacttg atgtgcttac
     3001 ctgggcaagg aacgggttag tagatgttat tgtaccaaca gcaagatggg ctacgacaga
     3061 taataatatg cctattgatt tctggaaaca aatccttgac gggaccggag ctatagttgc
     3121 agctggaatt gaaatattac tagaggctac aagagcaaat cccaaataca atgcatttaa
     3181 ttctcttgaa acagctagag gaagtgctta tacatatctt aacatgggtg ctgacaaaat
     3241 ataccttttt aattatatgg atcaaccatc acttttgtca cattctttag aatttaattc
     3301 tgctgttgat aaacgaaact accatgaact cctaataact atctgtgatc tggagactgc
     3361 ttcaaaattg ccccgaagga atgttgttac ataccatgac atagcagcac ctggtttacg
     3421 aaatggagat ctgcttccac tatgttgtag ttcacctaat tctaacggct caatgatctt
     3481 taagaatatc cgaatagaaa ctggtccaat caataaaaat ttaaaacgat ctttagtatt
     3541 aggcattgac tctaagagag aacttgactc ttcagatttt gatgtatatt ggaacacaaa
     3601 taaatgcaaa cttgtaggaa ttatcacctt atcagctccc aaaccaaact gtaaaacata
     3661 taaatttaaa atagagtgca gcgaaaagtc tcaagaagca tttcccagtg ctagtattat
     3721 agaaattgct tccaagaata ttccattttc tgtagaatgg gctgaaatac taattaatta
     3781 tcaaggctgt taacatgttt gagttagtac aatagcgaaa aattgttctt tgtcagcttt
     3841 ctgattccac tgttgatgta aagatgttca atttatcaaa aagcgatcat aatgatgtca
     3901 ttacagaagt tttcaaatca aagacaatcg tacttggatc tctaacagtg tgcaacggta
     3961 tccttgataa agttgaaggc tttgtacatc taatgaaagg acttaaattt tagaaaaaga
     4021 aggcggcatc ctttggttgt tatggttgga gtggcgaggg aacgaagact cttaatgaat
     4081 tatcggcaaa tgcggggttt gaagtgatcg acgaaggttc tggaagattg tggaatccca
     4141 atgaagaaag tcagcaagtt gctgttgaat atggcaaaaa gattgcaaag gcataaagca
     4201 tacagaaggc ataaagcata cagaaggcat aaagcataca gaaggcatag agcatataat
     4261 tggaaaaatt aggattctgc tacaaatata agaagcagaa ccctaagaat caataaggat
     4321 cagcagcaat aaggctccat atcaataatg ctccgcagta agaagccaaa ctatcaatat
     4381 tatcttactt aagtcatact acggaaaata aattttccta tagaattttc ctattcattg
     4441 tgacaaaatt atcattatac atggtgaaat ggatattatc acttgctgcg gcaggcagat
     4501 tgtcctttat ataattttct agcttatcta taaccttttt tgcgagtcat aatactttgc
     4561 tctatagtct cctggagcta ctgaaacata ctttttgaaa gctctgatat aactgaaacg
     4621 attattgtat cccgccattt ctgcaatctg ctcgatactt ttgtctgttt cggacaacag
     4681 gtgcttcgaa ttttttactc ttatttttga aacataatcc caaaaactta taccaatttt
     4741 ctctttaaaa tcttacagaa atatgaagaa ttataattaa aattgtatgc aattgtggca
     4801 agagatatct cttgcatata tttatcttcg atatattttt taatttcaga tatcagactt
     4861 tttcactgtc tttatcttga tcaatcgctt gaatagactc ggtgccagac actttttgca
     4921 gtgcatagag gaaacataat ttttttgctg ataccaagcg tatcagcaat ttgcgtgtta
     4981 aggggatgta tggggatgcg cttaaatatc ctgttaatta tggataacag tatagatttt
     5041 ctaatcaaat gatagatatt agcaatatac acagctggat gcaaggaata atcaatttta
     5101 aatggttgta actctatgct ttctgataaa tatgattatg aatgttaaat gtgttcttat
     5161 tactgctaaa caggcacttg tttatacaac atttaatgaa tatattaagg cacaacgatc
     5221 ttgacattgt tgagctggaa agataaaatc aatggcaaga agtagaactt tatggagtta
     5281 agcgctgtat ataaaaaatt aaga
//
LOCUS       DAOSDD010000276         5302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5523_length_5302_cov_5.475319, whole genome shotgun sequence.
ACCESSION   DAOSDD010000276 DAOSDD010000000
VERSION     DAOSDD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_5302_cov_5.475319"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1292
                     /gene="argJ"
                     /locus_tag="PK733_17330"
     CDS             90..1292
                     /gene="argJ"
                     /locus_tag="PK733_17330"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOJ12329.1"
                     /translation="MRVIEGGVTSPEGYFAAGVACGIKKDNKKDIAIVCSEDVAATAG
                     MFTTNIVKGHSVQVTMEHLKSGYATAVVINSGNANACIGKQGYKDAKEIAEITAQYLE
                     CDTENILVGSTGVIGVPLNMELIRPALEELVSNMSPDGGHDAVEAIMTTDLIPKEIAV
                     KLDLQGKSVVIGGMAKGSGMIHPNMATMIGVITTDANISRGLLNKALNDVVKHTFNRV
                     SVDGDTSVCDMVIIMANGMADNPAIINEDKNYNKFKDALNYVCTELARKIAADGEGAT
                     KLVEIKINEAPTEEDAYKVVSAIAKSPLVKTALFGEDANWGRILTAAGYSGAIFEPEK
                     TDIYIGDLLVCKKGIAVKFDEEKAKNILHEKEIIITVNLNQGNFSDRMWTCDFSYDYV
                     KINGSYRS"
     gene            1823..3307
                     /locus_tag="PK733_17335"
     CDS             1823..3307
                     /locus_tag="PK733_17335"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ12330.1"
                     /translation="MNLKLIKQKDFLLLILGKLVSLLGSNMQQFALSLYVLAITGSAA
                     IFASMLSISVLPRLLLSPIAGVFGDWFDRKKTIVGLDLINSIIIGIFAVIYIINGKIS
                     IPMIYILLILLEATEIFFSSAMSAVLPSMVGKEDLLDASSFNSLVMNIGNLAAPLIAA
                     LIYGAFGLKAILIFNSISFLLSAISEMFIIIPKKHKRPDKITIESFKTDLMEGISTIK
                     NNNLISTMISLGTIINFCVSPVFSVGLVFIIKEVLKSTDFQFSLFQMVISFSMLMAPL
                     LCGSYIKKIKIGRLCFASFVAISLLILVMAIIPSGFVLNFFSTNIIPYVFILIFSFMI
                     GIAATVANIALGTLFNKIVPLELMGRTSTVFNLAVTVFIPIGQMIFGVLYDIMSPGFI
                     IAISGVILIITIYKYKASLFNYDIDFNEIKSQLSSQDEVNSQLIKYDEVSSGLSMYDE
                     VKSGLSMYDEVKSGLSMCDEVKHELSKHNEVKYNWINTMKYNIK"
     gene            3343..4452
                     /locus_tag="PK733_17340"
     CDS             3343..4452
                     /locus_tag="PK733_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOJ12331.1"
                     /translation="MCFKFFLSQSRIYDFLFFPSLVFYLEEYNNLENGQNYKELVTDS
                     YLAFVKKVEDKLRPYMEDIEVFYTKQLMNQYDFMDLISKANSIFNYRNEKEYLDMLLS
                     LSEKEVNRSIIYSLMLIGESHNDYGYSDEVMEKAEEISRNKDQLITYIKDLPIEAAQK
                     WNLFLILEQPLKYMKKYVELMLKLLPVFEEIYFPYEDEVKTYGKSLVDFLNENGTKGL
                     DDISYSLLNTEVIDEKEINIIISIMFSYAISIVTDIRGTYLAWGLKMEEAFKAMKEIN
                     ENKTNARVQIFKNLGDKTRYEVLKLIASGETSTKKIAKALGVSSATVSYHISNFLTSK
                     VIKMDKTDNKCGYAVDYELLEEALKALKEDLKFPK"
     gene            complement(4464..4637)
                     /locus_tag="PK733_17345"
     CDS             complement(4464..4637)
                     /locus_tag="PK733_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12332.1"
                     /translation="MAGMLLTSCTVMPVIQLKNNNKTSIDTDKKLNKCTLKVLIVILT
                     LAKRSSVEFKGED"
     gene            4775..>5302
                     /locus_tag="PK733_17350"
     CDS             4775..>5302
                     /locus_tag="PK733_17350"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12333.1"
                     /translation="MEMIRKKGMPIGISDYKNLIDRNAYYVDKTLLIKDIINDESETI
                     ILIRPRRFGKTLNLSMLYYYFNNSGDDNTYLFKDKKIFQQEERYTSELGKYPVIYLTF
                     KDIKQTNWESSCEKIIMEISSLFIKHKYLLNSNVLEGSEKTIYHEVFNGTGDIGIYQN
                     SLKLLSKALEQYHQQK"
BASE COUNT         1879 a          726 c          968 g         1729 t
ORIGIN      
        1 aggtgcatgt aataattatg tgttattatg ttaataagta ataaggtgat tttctatctt
       61 taatgctaat ttgacgggag ttgattgtta tgcgtgttat agagggagga gtaacttccc
      121 ctgaaggtta ttttgccgct ggggttgcat gtggaattaa aaaagataat aaaaaagata
      181 ttgcaattgt ctgttctgaa gatgtagcag caacagcagg catgtttacc acaaatattg
      241 tgaaagggca ttctgttcaa gtcacaatgg agcacttaaa aagcggttat gctactgctg
      301 tagttataaa tagcggcaat gcaaatgcat gtattggaaa gcagggttat aaagatgcaa
      361 aggaaattgc ggaaataact gctcaatatc ttgaatgtga tactgaaaat atccttgtag
      421 gctctacagg tgttataggt gtacccctta atatggagct tatccgccct gcccttgagg
      481 aattggtatc taatatgtcc cctgacggcg ggcatgatgc tgtagaagca ataatgacga
      541 ccgaccttat tcccaaggaa attgctgtta agcttgattt acagggtaaa tccgtagtaa
      601 taggaggtat ggcaaagggt tcaggaatga tacacccaaa tatggctaca atgataggtg
      661 ttataactac agatgccaat atatcaaggg gacttttaaa caaagcgctt aatgatgttg
      721 taaaacatac atttaaccgg gtttctgtgg atggagatac aagtgtttgt gatatggtca
      781 taattatggc aaacggcatg gcagataacc ccgctataat caatgaagac aaaaactata
      841 ataaattcaa ggatgccctt aattatgtat gtactgaact tgcaagaaaa attgctgctg
      901 atggagaggg tgccacaaaa ctggtagaaa taaagataaa tgaggcacca actgaagagg
      961 atgcttacaa ggttgtaagc gctatcgcaa aatcacccct cgttaaaact gcattgttcg
     1021 gcgaagatgc caattgggga aggattctta cagctgctgg ctattccggt gctatttttg
     1081 aacctgaaaa gacagatatt tatataggag atttactggt atgtaaaaaa ggtattgctg
     1141 ttaagtttga tgaagaaaaa gcaaaaaaca ttttgcatga aaaagaaatt ataataacag
     1201 tcaaccttaa ccaaggcaat ttttcagaca gaatgtggac ctgcgacttt tcatatgatt
     1261 atgtaaagat aaatggaagc tacaggagct aaaataagga accgtataaa ctgtataaat
     1321 aggtaggaac cgtatgggta tatatagcag taatatagtc cacacgattc ctatcttacc
     1381 atcttaatag taattgtcag tatttttgta cttcttattt ttttattctt ttcatttttg
     1441 atttatatat tagaggtatt atttagttgt aaacattagc actttctacc atatcacttt
     1501 ttaccattta taacgaatat ccaaattttc atacatagtt cgtacaaaat ccaattgtta
     1561 agttatgagc gtggacacat tttcttccta ttatgcaaaa tatagaaaat gtaacctatg
     1621 atcattaaac atatatgcaa tatataaacc acatgggtat tgtaataatg ttgccaggca
     1681 tttatataat cacctaatat cgtcttcaaa ttaaaccaat atattattta gtatattaat
     1741 tagataatta tctaatttta atattgacat tagataatta tatgcatata atgaagtaaa
     1801 gagataattc cggaggcgta gtatgaattt aaaacttata aagcaaaaag attttctact
     1861 gcttatattg ggtaaattag tatctttatt gggaagcaat atgcagcaat ttgcattatc
     1921 attatatgta cttgcaataa ccggttctgc tgctatcttt gcttcaatgt tatccatatc
     1981 cgttttaccc aggctgctgt tatcaccaat agcaggagtt tttggagatt ggtttgatag
     2041 aaaaaagact atagtaggct tagatttaat aaattcaatt attattggaa tttttgcagt
     2101 tatttatatt ataaatggaa aaatatccat tccaatgatc tatatcctgc ttattttatt
     2161 ggaagcaacc gagatttttt ttagttccgc catgtctgca gtattaccaa gcatggtagg
     2221 taaagaagat ttgcttgatg caagttcatt taattcttta gtaatgaata ttggcaattt
     2281 agcagcgccg ttaattgcag ctttaattta tggtgcattt gggttaaaag ctattttgat
     2341 atttaactcc atcagctttt tactatcagc aattagtgaa atgtttatta ttataccaaa
     2401 gaagcataaa agaccagata agataactat tgaatctttt aaaacagatt taatggaagg
     2461 aattagcaca attaaaaata ataatttaat atctacaatg ataagtttag gtactattat
     2521 taacttttgt gtctcgccgg ttttctcagt tgggctggtt ttcatcatca aggaagttct
     2581 aaaatctacg gattttcaat ttagcttatt tcaaatggtt atatcttttt ctatgctaat
     2641 ggcaccacta ctttgcggta gctatattaa aaagataaaa attggcagat tgtgctttgc
     2701 aagctttgtt gccatatcac tgctaatatt ggtcatggca attattccat caggttttgt
     2761 gttaaatttt tttagtacaa atattattcc atatgtattt atattaattt tctcatttat
     2821 gattggtata gctgcaactg ttgcaaatat tgctctaggg acgcttttta acaaaatagt
     2881 acctttagaa ttaatgggca gaacttcaac agttttcaac ctggcagtga ccgtatttat
     2941 ccctatagga caaatgattt ttggtgtttt atatgatata atgtctccag gttttataat
     3001 agccataagt ggggtaatac taattataac aatttataaa tataaggcat ccttatttaa
     3061 ttatgatatt gatttcaatg aaataaaatc ccaattgagc agtcaggatg aagtgaattc
     3121 ccaactgatt aagtacgatg aagtgagttc aggactgagt atgtatgatg aagtgaagtc
     3181 tggactgagt atgtatgacg aagtgaagtc cggattgagt atgtgcgacg aagtgaaaca
     3241 cgaattaagt aagcacaatg aagtaaaata caattggata aacacgatga aatacaatat
     3301 aaaataaatt agtactacta tgccaatgag gaggtattgt gaatgtgctt taaatttttt
     3361 ctaagtcaga gcagaatata tgatttttta tttttcccca gcctggtatt ttatttagaa
     3421 gaatataaca acctggaaaa tggtcaaaac tataaggaat tggtgacgga cagctatctc
     3481 gcatttgtaa aaaaagtaga agataagctg aggccctata tggaagatat agaagtattc
     3541 tatacaaaac aattgatgaa ccaatatgat tttatggatc tcatttcaaa agctaacagc
     3601 atatttaatt acagaaatga aaaggaatat ttggacatgt tgctgtcttt aagtgaaaag
     3661 gaagttaaca gaagcattat ttactcgtta atgttaattg gtgaaagtca taatgactat
     3721 ggctattctg atgaagttat ggaaaaggct gaagaaataa gtcggaataa agatcaacta
     3781 attacatata taaaggattt acccattgaa gctgctcaga aatggaattt atttttaata
     3841 ttggagcaac ctttgaaata tatgaaaaaa tatgttgaat taatgctcaa gctattgccg
     3901 gtttttgaag aaatatattt tccatatgag gatgaagtaa aaacttatgg gaaaagcctt
     3961 gtggattttt taaatgaaaa tggtaccaaa ggattagacg atataagcta ttccttatta
     4021 aatacagaag ttattgatga gaaagaaatc aatatcatta tttctataat gttttcttat
     4081 gctatatcaa tagtaactga tatacgaggt acatatctgg cttggggttt aaagatggag
     4141 gaagccttca aggcaatgaa agaaataaat gaaaataaaa ctaatgcgag agttcagata
     4201 tttaaaaacc ttggagataa aaccagatat gaggtgttaa aattgatcgc ctctggagaa
     4261 acttccacaa aaaagattgc aaaagctttg ggggtgtcca gtgcaaccgt atcataccat
     4321 ataagtaatt ttcttacatc aaaagttata aagatggata aaaccgataa taagtgtgga
     4381 tatgcagtag actatgaatt gctggaagaa gccttaaaag ctcttaaaga ggatctgaag
     4441 ttcccgaaat aagccggaat aagttagtct tcccctttga actcaactga actcctttta
     4501 gctaaggtta aaattactat cagcactttt aaagtacact tatttaattt tttatcagta
     4561 tcaatacttg tcttgttatt attttttaac tgtattactg gcatcacagt acaggatgtc
     4621 agaagcattc ctgccaatta aaatgccctt cacattttgt aacaagcctg cttaaattcc
     4681 taaatgtata attttactct tatggtgagt tattaataaa agtgatataa tatattagag
     4741 ataaaattat tcatgtaaaa aggcaggtat tgatatggaa atgataagaa aaaagggaat
     4801 gcctataggc atatcagatt ataaaaattt aatagataga aatgcatatt atgttgataa
     4861 aacgctactt attaaggaca ttatcaacga tgagagcgaa acaattatac ttatcaggcc
     4921 aagaaggttt ggcaaaaccc ttaatctttc catgctttac tactatttca acaattctgg
     4981 tgatgataac acttatctat ttaaagataa aaaaatattt caacaggaag aaagatatac
     5041 ctctgagttg gggaaatatc ctgtgatata tctgacattt aaagatatta aacaaacaaa
     5101 ctgggaatca agctgtgaaa aaataattat ggaaatatca agcttgttca ttaagcataa
     5161 atatctcctt aattcaaatg tattggaagg tagtgaaaaa actatatatc atgaagtgtt
     5221 taatggtact ggagatattg gaatatatca gaatagctta aaactgttat ccaaagcttt
     5281 agagcaatac catcagcaaa aa
//
LOCUS       DAOSDD010000277         5300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5525_length_5300_cov_3.567588, whole genome shotgun sequence.
ACCESSION   DAOSDD010000277 DAOSDD010000000
VERSION     DAOSDD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5525_length_5300_cov_3.567588"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK733_17355"
     CDS             <1..483
                     /locus_tag="PK733_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12334.1"
                     /translation="ESLEKQKKAVEKAIQQEIIGFNNEYICLTSVKGIGPVIAAGLIS
                     EIGGISRFDDDNALAKFSGLYWSEYQSADFTVEDTYLKRTGNEYLRYYFVQAADQLRK
                     YLPEFGTYYYRKFKESKTHKHKRALVLTARKAVRLIFALLREGKLYKPPAVKGEGAKT
                     "
     gene            complement(562..1005)
                     /locus_tag="PK733_17360"
     CDS             complement(562..1005)
                     /locus_tag="PK733_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOJ12335.1"
                     /translation="MDEKLIAPCGMNCCLCIAYQFKENDINRRGFHKKYCPGCIPRGE
                     NCTHMRDACELLAKGSIRFCFECENFPCKRLKALDRRYRTKYHMSMIENLKYINEFGM
                     EEFLKKERDKWLCTECGATICCHNGLCLNCNIDTLLMNKKYRWGE"
     gene            complement(1211..1699)
                     /locus_tag="PK733_17365"
     CDS             complement(1211..1699)
                     /locus_tag="PK733_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12336.1"
                     /translation="MKTLNELIEAYTNHLQQGEIQIAYKGILEFLGKLRADFIKKHPH
                     YDVSSIYQGYMDMSYFSLSTKSLKDKGLKIAIVYLHDKGDFEVWLSARNREISKQYES
                     VFCGKSFDGITVFHDDNNQDAIIEYTLTSSPNFEDQVILTDIIEQGIEAFITAVSNLL
                     IR"
     gene            complement(2082..2978)
                     /locus_tag="PK733_17370"
     CDS             complement(2082..2978)
                     /locus_tag="PK733_17370"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOJ12337.1"
                     /translation="MGNIAESVLAKLKNESKKKGISLQQLLNLFCQEEFIRRLSNSNY
                     KYNLILKGGFLLYSISKFTTRPTIDADYLLKNYSNDINTIEKLVREVISICNKNEFIE
                     IKIRNLGIISEFKEYHGVRVNLIGIIGRTRTPFSIDFGVGDIVIPSPVERTLPALLSD
                     FEEPRILTYSLEYTVAEKIDAIISLMETTGRMKDFYDIYYIATTFDFEGRKLQEAIYE
                     TLTNRGRAYEIDSISDIFRLTKDLEILKRWNNFCSKILNYELNFTDVVNVIISFISPP
                     YESILVEDEFFKDWRCKEGRYF"
     gene            complement(2978..3574)
                     /locus_tag="PK733_17375"
     CDS             complement(2978..3574)
                     /locus_tag="PK733_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOJ12338.1"
                     /translation="MNRKDIINKAFRKNGGILRTSELNNLGLSSRQINKLLESGDIIR
                     IKRGFYELSDSIYPEEVVIARLFPNSVIFLESALIIYDYTDRIPAAWQIAVDRDSEKT
                     QYKIDYPQINVFYIEPKLLEVGVDIIQIEGVDIKIFNRDRTICDVLRYENKLEREVFS
                     KAIQRYVKDPKVNISRLLEYSDIFNIKNKVQTYIGVWL"
     gene            complement(3717..3902)
                     /locus_tag="PK733_17380"
     CDS             complement(3717..3902)
                     /locus_tag="PK733_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12339.1"
                     /translation="MYRYRCRDCAYEEDVPGYVIEEFIWWEEMDEEYAKTKDSRMPTM
                     ICPRCEGTLVFKEKTVD"
     gene            complement(3868..5202)
                     /locus_tag="PK733_17385"
     CDS             complement(3868..5202)
                     /locus_tag="PK733_17385"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12340.1"
                     /translation="MKKAEYLKQLVKEKDVKVSQILKDHWTAFKKTKLYKVPEDMRKS
                     VVEAVEKALSCGDISKGYTKYKCIECDNDHEVIVGHSCKSRFCNRCGKVYVENWVNKQ
                     VETIFDVTHRHTVFTVPEELRKEIYWNRDILKDISDGIYELICYWYEKMGKSRKYMPG
                     VITVVHTFGRSLDFNPHIHALVTEGALDKDNKWKEVKYIPYEYLRKAWQKVLMDIVKK
                     KLVPKKPQLKKLINELYKRYPQGFYVHAETKMDGARRAAQYIGRYLARPAIAEYRIIS
                     YDGDTVKFWYEDHTTGEIVERELPVLDFIGKIISHIPKKHFKMVRRYGLYRRDLNKHA
                     KKSVEMQAYYKRKPKENVKIEKKTWKQRIIDSFGRNPIKCKKCGKEMELWEIWDARYG
                     TIYHIFDEWKLLKEVPDLNAKGKENIRRRDTFRRSEGDKQGYVQISLPGLCI"
BASE COUNT         1529 a         1124 c          699 g         1948 t
ORIGIN      
        1 gaatcacttg agaaacagaa gaaagctgtg gaaaaagcca tccagcaaga aatcatcgga
       61 tttaacaatg aatacatttg cctaacctct gttaaaggaa ttggacctgt tatagcagct
      121 ggcttaatat cagagatagg tggtatttca aggttcgatg atgacaatgc acttgcaaaa
      181 ttctcaggtc tttattggtc tgagtaccag tctgccgatt ttacggttga ggacacatat
      241 ttgaagcgga ctggtaatga atacctcagg tattactttg tacaagctgc agatcagcta
      301 agaaagtatt tacctgaatt tggtacttat tattatcgta aatttaaaga atctaagact
      361 cacaagcata aacgtgcgtt agttttaact gcccgtaaag ctgtaaggtt gatctttgct
      421 ctgctgcgcg aaggaaaact ttataaaccg ccagctgtga aaggagaggg tgcgaaaaca
      481 tagaattttt tgccatttcc atatttgtaa aaataaacta ctccgattca tactgcttat
      541 caatggtcat tcctttcacc tctattcacc ccaccgatat tttttattca tgagtagcgt
      601 atcaatatta caatttaaac atagtccgtt atggcaacaa atcgtcgctc cgcactctgt
      661 gcaaagccat ttatcacgtt ccttctttaa gaattcttcc atgccaaatt cgtttatgta
      721 tttgagattt tctatcatgc tcatatggta ttttgtacgg tagcgcctat ccaatgcctt
      781 caatctctta caagggaagt tttcgcattc aaaacaaaac cggatgctac cttttgccaa
      841 caattcgcaa gcatctctca tatgcgtgca gttttctccc ctcggaatgc atcctggaca
      901 atattttttg tgaaaccctc gtctgtttat atcattttcc ttgaattgat aagcgatgca
      961 taaacagcag ttcatcccac aaggggctat caatttttca tccatagata cacctcttca
     1021 tttaacacac atgtatcatg cactctcaac ctcggcaagc tcaaagctga tcaacactgt
     1081 ttgtgctcac acgaaagtga atgcctgctc gcttttcccg ctttggaggc aatggtggga
     1141 tataatgaat tattttatta tcagccataa caaaacctcc tcaattacaa gtgcattgac
     1201 aaataaaaat ctatcttata agaagattgc ttacagctgt tatgaacgct tcgatacctt
     1261 gctcgataat atctgtcaaa attacctggt cctcaaaatt cggtgaagat gtcaatgtgt
     1321 attctatgat ggcatcctga ttattatcgt catgaaatac tgtaatccca tcaaaggatt
     1381 taccgcaaaa tacagactca tattgcttgg atatttctct gttacgagca gaaagccata
     1441 cttcaaagtc tcccttatca tgtaaataga caatggctat ttttaagcct ttatccttta
     1501 acgacttcgt gctcaaagaa aaataggaca tatccatgta gccttgatat atactgctta
     1561 catcgtaatg tgggtgtttc ttgataaaat cagctcttaa tttccctaaa aattctaata
     1621 ttcctttata cgcaatttgt atttcaccct gttgtaaatg atttgtatac gcttcaatta
     1681 gctcattcag agtcttcata tttcgcccct taaattttat ttttcgcaac gttttatcta
     1741 acatccaacc tgaccacaca accctgccaa attcaaaaaa catctaaatc gaccattagt
     1801 taacccctga catctaaact gaccactcgt taaacaccga catctaaatc gctctgtcac
     1861 gtctaaaatc atgcctttgg acttatttcc acgaaccgat attttcacta tttatgctta
     1921 tcaaatttta tctttaaaac ctcgaaatgc ccttatatca agcttttttt acactcattc
     1981 tttcaccctt gacatcaata atactgtctc aacgtgcctt aagtggtcaa taaagatgtc
     2041 gtagggggat agtatcccat tgatggaaga ataaatattt tttaaaaata tcttccttct
     2101 ttacatctcc aatctttaaa aaattcatct tcaactagaa tggattcata tggtggtgat
     2161 ataaaactaa ttattacatt aactacatct gtaaaattaa gctcatagtt taatatctta
     2221 ctacagaaat tattccatct ctttagaatt tctaaatcct tagttaatct gaaaatatca
     2281 gaaatagaat ctatttcata tgctcttcct ctatttgtta aggtttcata aattgcttct
     2341 tgcaactttc ttccttcaaa atcaaatgtt gttgctatgt agtatatgtc ataaaaatct
     2401 ttcattcttc ctgtagtttc cattaatgat ataatagcat caattttttc tgctactgta
     2461 tactctaaag aataagttaa aattcttggt tcttcaaagt ctgaaagcaa tgctggtaga
     2521 gtcctctcaa ctggtgaagg tataacaata tctccaactc caaagtctat actaaaaggt
     2581 gtccttgttc ttccaatgat tccaatcagg tttactctaa caccgtggta ttccttaaat
     2641 tcactaataa ttcctagatt tctaatctta atttctataa actcattttt attacatatg
     2701 gatattactt ctctaacaag tttttctata gtatttatat cattagaata gttttttaat
     2761 aaataatctg catcaattgt tggtctagtg gtgaatttac ttattgaata taataaaaat
     2821 ccacccttaa gaattaaatt atatttataa ttcgaatttg aaagccttct gataaactct
     2881 tcctgacaaa acaagttcaa tagttgttgt aatgaaattc cttttttctt tgactcattt
     2941 tttagtttag ccaatacaga ttctgcaata tttcccatta caaccacact cctatatatg
     3001 tctgtacttt gtttttaatg ttaaatatat cagaatactc aagtagcctt gaaatgttta
     3061 cttttggatc cttaacatat ctttgaatag ctttactaaa tacttctctc tctaatttat
     3121 tttcatatct aagaacatca catatggtac ggtcacgatt aaatatttta atatctacac
     3181 cttctatttg aattatatca actccaactt ctagaagttt aggttctata taaaacacat
     3241 ttatttgtgg atagtcaatt ttatactgtg ttttttcact atctctgtct acagctattt
     3301 gccatgctgc tggtatccta tctgtatagt cataaattat taacgcactt tcaagaaata
     3361 tcactgaatt aggaaatagt cgtgctatta caacttcttc cggatatatg ctatctgaca
     3421 gctcataaaa cccacgctta attcttatta tatctccact ttctagaagt ttatttattt
     3481 gtctacttga aagccccaaa ttattaagtt ctgaagttct tagtattccg ccattttttc
     3541 taaatgcttt gttaattata tcttttctat tcatattcgc acctcgatat ccgcactcta
     3601 aatagtattg cgggtatttc aatgcaatta taccataggt ttttatcata agtaaagctt
     3661 ttttattgca aaagaacaaa gttgccctac gggcaattta taagaacggt agattattag
     3721 tctaccgttt tctctttaaa tacaagcgta ccttcacatc ttggacagat catggtaggc
     3781 attctactat ccttggtttt agcatattcc tcatccatct cttcccacca aataaattct
     3841 tcaataacgt atccaggtac atcttcttca tatgcacagt cccggcagcg atatctgtac
     3901 atatccttgt ttatctcctt cggatcgtcg aaaggtatct cgtcgtcgta tattttcttt
     3961 tcccttggca tttaggtctg gtacctcctt tagcagcttc cattcatcaa atatgtgata
     4021 tatcgtacca tatcttgcat cccatatctc ccataactcc atttctttgc cacatttctt
     4081 acactttata gggtttctcc cgaaactatc tataatcctt tgcttccatg tctttttttc
     4141 tatcttaaca ttctcctttg gctttctctt gtaataagcc tgcatttcaa ctgatttttt
     4201 tgcatgcttg tttaaatctc tacgatataa accatatctc cttaccatct taaaatgttt
     4261 ctttggtata tggctaatga ttttccctat aaagtccagt accggtaatt cccgctctac
     4321 gatttcaccg gtagtatggt cttcatacca gaacttaact gtatcaccat catagcttat
     4381 tatcctatat tctgctattg caggtctcgc tagatatctg cctatatatt gtgccgctct
     4441 tcttgctcca tccatttttg tctctgcatg tacgtaaaaa ccctgcggat accttttata
     4501 tagctcattg atcagctttt ttagttgtgg ctttttaggt accagtttct tcttcactat
     4561 atccatcaat accttctgcc aggctttcct tagatactcg tacggtatat attttacttc
     4621 tttccatttg ttatccttat caagtgcccc ttctgttact aatgcgtgta tatgcgggtt
     4681 aaaatccaaa gacctaccaa atgtatgcac tactgttatg actcccggca tatatttacg
     4741 gctcttcccc attttttcat accagtaaca tatcaattca taaattccgt cactaatatc
     4801 ctttaagata tcccggttcc aatatatttc ctttcttaat tcctctggta ctgtaaacac
     4861 tgtatgtcta tgcgttacat caaatattgt ctcaacctgc ttgtttaccc agttttctac
     4921 atacactttc ccacatctat tacaaaatct acttttacag ctgtgcccta caattacttc
     4981 atgatcattg tcacattcta tacatttata ctttgtataa cctttgctaa tatcaccgca
     5041 gcttaacgcc ttttcaacag cctccactac gctttttcgc atatcttcag gaaccttgta
     5101 cagttttgtt ttcttaaacg ctgtccaatg gtctttaagt atctgtgata cttttacatc
     5161 cttttcctta actaattgtt ttaaatattc tgctttcttc atgtttatat tctactggaa
     5221 atattagtat tattcaaccc taccccttga aatcagctat ctttaaaatt tcaaaaccac
     5281 catagtttcc tatacatttt
//
LOCUS       DAOSDD010000278         5295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5535_length_5295_cov_5.694275, whole genome shotgun sequence.
ACCESSION   DAOSDD010000278 DAOSDD010000000
VERSION     DAOSDD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_5295_cov_5.694275"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1810
                     /locus_tag="PK733_17390"
     CDS             305..1810
                     /locus_tag="PK733_17390"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOJ12341.1"
                     /translation="MGFSKNTTERNIVIYNEFIIINKLSHCSVVEFNTLYGVKKIAII
                     LNACNRIIYVLDYQYNHDALNILEEYNIIFSTLKADKTFYLKGEYELNIREYIINFPY
                     SIKYNILKNTLHINKINNVAGSLKIILNTTNRQYRMGAFKHKKIGTGKDACVFYEIDN
                     VQTELANLASKITNEKQRKYRESHQYRKSYDMIQEYDEGKENKLESNENKAKRKYTRK
                     NSKVITYKINNEVFDYEKTNNIIRKDISHELEAVSSDKIIINSSESYTVNNYNHNPKN
                     NKTNTNKEINNDLLINKGNLEPEKKLRTFIATNRNLTIVKYLKNLYENKCQICAESIE
                     ISNNQFLCEVHHIIPLGIHNGADVLENMIVLCPNHHAMFDRGAITIDLDKKIVIHFNK
                     SDPLNNKPIELKHEINKSYIDYHNKNIFINYTHYQQYENNDINSKNIIVDYGDIVTLQ
                     DTNTKEVFNVKIEDKFNREFMKDIEKILLHKALFEYINYGGFVYQITEILK"
     gene            2287..2562
                     /locus_tag="PK733_17395"
     CDS             2287..2562
                     /locus_tag="PK733_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12342.1"
                     /translation="MFIINVNDIFNYLHNVPDKSNDASYEIKYIHYLISSGTAKIGEI
                     NFELLAQTDLEDYANYYYQQSQNYSNIEKTVFKLIATKPIYSSEAFM"
     gene            2625..3263
                     /locus_tag="PK733_17400"
     CDS             2625..3263
                     /locus_tag="PK733_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12343.1"
                     /translation="MSIIKRNKYNYWGSTYDDTGLHNTNFQIIKILKLMYDTGICLYI
                     DDPLDEMCMWDNMYEVFYESFELLADDSGFYITDNDYSEIITKFYNKQIFYFFKNLQK
                     YYSIFGCRKGIHYTKLYLTAQSNFMRNVYNCDYYMIYNYKHNAYAGIKFIFFDYFDYY
                     SDFIENLIVLISFFKNANAEMRKEIYSYQKQKYSEKPERTKRQGVDSYATAS"
     gene            3247..3951
                     /locus_tag="PK733_17405"
     CDS             3247..3951
                     /locus_tag="PK733_17405"
                     /inference="COORDINATES: protein motif:HMM:NF025815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12344.1"
                     /translation="MQLQAKEMVIRLKHDATVICEEIFENNLVVSKHISIEDLCSAVN
                     NSIRLEGVETGILPEGCIYFKEDMDKNRYIVLKYNNAVDFSLYETVYKNLPLPTLLFG
                     FKIQKNGHINKRMITCIKGNKIKMDTKLCYYPFSNVYNNNFNICLGNNRLKDIKSIIQ
                     IEAYPYYIMSLTNNNDLYNQKHNSCNFEYRELLDYISKKGEFNDDLLVERDITVRHFI
                     EALIDIKIKENESDIA"
     gene            4488..4871
                     /locus_tag="PK733_17410"
     CDS             4488..4871
                     /locus_tag="PK733_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOJ12345.1"
                     /translation="MDRGKGIAYCGLACCVCSENATCAGCRNEGCKNKEWCKSFNCCK
                     IKGLNGCWECSEFPCKNQMLDKLRVRTFAKFIAEYGEEKFMDAIEKNELDGIVYHYEG
                     QIIGDYDNTQGEDEIRQLLLRGLRI"
BASE COUNT         2125 a          637 c          833 g         1700 t
ORIGIN      
        1 ataataaata atatctagtt ttattatata acatatactt attattaccc agtatattat
       61 actgaaaatt ttacatattt aatgaatgta taaaaactga ttttaatcat ctattataca
      121 ataagcggta taattggaat agaaaacata ttacttaggt ggcaacacta gaaatgcaag
      181 tgcatttgct tatattgcag tatagtaaca ctgctataca ggagtaaatt tagagcagtt
      241 acactgctaa aagtctacaa taaatatttt atatttattg caatatatat tgtgagggga
      301 atgtatggga tttagtaaaa atactactga aagaaatata gtaatatata atgaatttat
      361 aataattaat aaattatctc attgcagcgt agttgaattt aataccctgt atggagtaaa
      421 gaagattgca ataatattaa atgcttgtaa taggataata tatgttttag attatcaata
      481 taatcatgac gcgttgaata ttttggagga atataatatt atatttagca ccttgaaggc
      541 agataaaacc ttttatctaa aaggcgaata cgagctaaat attagagagt atattattaa
      601 tttcccttat agtataaaat ataatatact aaaaaacacc ttacatatta acaaaattaa
      661 caatgtagca ggttcactta aaataatttt aaatactact aatagacagt atcgaatggg
      721 tgctttcaaa cataaaaaaa taggaactgg caaagatgca tgtgtatttt atgaaataga
      781 taatgtacag acagaattag caaacctagc ttcaaagata actaacgaga aacaaagaaa
      841 atatagagaa agtcatcagt atagaaaaag ttatgatatg atacaagaat atgatgaagg
      901 taaggaaaac aaattagaga gcaatgaaaa taaagcaaaa agaaagtata caagaaaaaa
      961 tagtaaagtt ataacttata aaatcaataa tgaagtattt gattatgaaa aaaccaataa
     1021 tattataaga aaagacatat ctcatgagtt ggaagctgta tctagcgata aaattattat
     1081 aaatagtagt gagagctata cagttaacaa ttataaccat aatcctaaaa ataataaaac
     1141 aaatacaaat aaggaaatta acaatgacct gctaattaac aaaggtaatc ttgagccgga
     1201 gaaaaagcta agaactttta ttgcgacaaa tagaaactta acaatagtaa agtatctaaa
     1261 aaatttgtat gaaaataagt gtcagatatg tgcagaaagt attgaaattt ctaataatca
     1321 atttttatgc gaagtacatc atattatacc tttaggaata cataatgggg cagatgttct
     1381 tgaaaatatg atagtattat gtcctaatca tcatgcaatg tttgacagag gagcaataac
     1441 tattgatctt gataaaaaga tagtaattca ttttaataaa tctgatccat taaataataa
     1501 gcctattgag ttaaaacatg aaattaacaa gagttatatt gattatcata ataaaaacat
     1561 ttttattaac tatacacatt atcaacaata tgaaaacaat gatattaact ctaaaaatat
     1621 aatagttgat tatggggata tagtaacatt acaagatact aatactaaag aagtattcaa
     1681 tgttaagatt gaagataaat ttaatagaga attcatgaag gacatagaaa aaatactatt
     1741 acataaagca ttatttgaat atattaatta tggcggattt gtataccaaa taacggagat
     1801 tttaaagtaa ttgcttgcat tattaatgaa agtattctta ttgaagtaaa aggattttgg
     1861 gatagaatga gtttacataa agtatatgcc tataaaacaa agaaggttat gatcggtatt
     1921 aaaatgttaa taacaaaata tttactgaaa gtagtttata tataattgtt agtgatattt
     1981 attctcttta gaaaatatat tccaaactaa tacctaattg agaaatacta ggaaatattt
     2041 atacaataca agagatgctt gtttaaaaaa tatatttact gtataataca taatgtaata
     2101 gtaattcatt taaaatatct ttttcatgga tatttaggtt ttaacacgaa attaaggtct
     2161 aggggaaatt atattctcta ggcctttttg tctttaaaaa gcaaatcaat agttcacttt
     2221 tattttgcca atagtgaata aaaaataatt ttcgcaccat gcgaaaaaat aaaaaagggg
     2281 ggacttatgt ttataattaa tgtaaatgac atattcaatt acttgcataa tgtacctgat
     2341 aaaagtaatg atgctagtta cgaaataaaa tacatccatt accttatttc gtcagggaca
     2401 gctaagattg gggaaataaa ctttgagttg ttagctcaaa cagacctaga agattatgct
     2461 aattactatt atcaacagtc acaaaattat agtaatatag agaaaacagt ttttaagcta
     2521 atcgcaacaa aaccaatata tagttcagaa gcctttatgt aagaaaataa aaaaagtatt
     2581 attttcgcat cgtgcgaaaa atatagtaaa gaaaggattg caatatgtct ataataaaac
     2641 gtaataaata taattattgg ggatcaactt atgacgatac tggcctacat aatacaaatt
     2701 ttcagattat aaaaatactc aaacttatgt atgatacagg catatgtcta tatattgatg
     2761 atcctctaga cgaaatgtgt atgtgggaca acatgtatga agtattttat gaaagctttg
     2821 aattgttagc tgatgactcc ggtttctaca tcacagacaa tgactacagc gagattataa
     2881 ctaaatttta taataaacag atattttatt tttttaaaaa cttacaaaaa tattatagta
     2941 tttttggttg tagaaaagga atacattaca ctaagttata tctaacagct caaagtaact
     3001 ttatgagaaa tgtttataac tgtgattatt acatgatata caattataag cataatgcat
     3061 atgcaggcat aaagtttata ttctttgatt attttgatta ttatagtgat ttcatagaaa
     3121 atcttatagt gttgatatca ttttttaaaa atgctaatgc agaaatgcga aaagaaatat
     3181 attcatacca aaaacaaaaa tactctgaaa aacctgaaag aactaaaaga caaggagttg
     3241 atagttatgc aactgcaagc taaagaaatg gttataagac tcaagcatga tgcaactgtt
     3301 atctgcgaag aaatctttga aaacaacttg gttgtcagta aacatataag cattgaggat
     3361 ctatgtagtg ctgtaaacaa ctctatcagg ctcgaaggcg tagaaacagg aattttgcca
     3421 gaaggctgca tatatttcaa agaggatatg gataaaaata gatatatcgt gttaaaatat
     3481 aataatgcgg ttgactttag cttatatgaa acagtatata aaaatttgcc acttcctaca
     3541 cttttatttg gttttaaaat acaaaaaaat ggtcatataa ataaaagaat gataacttgt
     3601 attaaaggta acaaaataaa aatggatact aagctctgtt attacccatt ttccaatgtt
     3661 tacaacaata attttaatat ctgtttgggt aataatagat taaaagatat taaaagtatt
     3721 atacaaattg aagcatatcc ttattacata atgtctctga ccaacaataa tgatttgtat
     3781 aatcaaaagc ataatagttg caattttgaa tacagggaat tacttgatta catttcaaaa
     3841 aaaggtgaat tcaatgatga tttattagtt gaaagagata ttacagttag gcatttcatc
     3901 gaagccctaa ttgacattaa aataaaagaa aatgaatccg atattgcctg aatattccgg
     3961 tgcactttgc gacacctgtc cggttattga gagtcattta taatttgctc gtttttgctt
     4021 tcattaaggc tactggaaac atgtacattt gtaaaggctg ctacgcatag ctgaagcgac
     4081 aagaccttga caaatataca tgtttccaag aattattagt caatcgaaaa caaggtggct
     4141 ctgtaccaac ggaatagtgg ctctatcaga caaggatatt cagttgtcgc ccaaattgta
     4201 taaaaaatta tataaaaagg gtttttaaca ttaaatatag tataatgatt tcataggata
     4261 gatagttaaa aatatattac tatttatcct atgtttttat ttattctata attaataaat
     4321 gcattagaag aaaacaaaca attaagtgaa cagtttaaaa ttagtaatac tgatatttat
     4381 aaaagtatat aaatacatgg taaacaatgg tataatttat atgtttgttc tttcacaaat
     4441 gtaaaagatt gcaaactaag gactatttat ataacgggag gctgaacatg gatagaggaa
     4501 aaggcattgc atattgtgga ttagcgtgtt gcgtttgtag tgaaaatgca acttgtgctg
     4561 gttgcagaaa tgaaggttgt aaaaataaag aatggtgcaa atcttttaat tgttgcaaaa
     4621 taaaaggtct aaatgggtgc tgggaatgtt cggagtttcc ttgtaaaaat cagatgttgg
     4681 acaaacttcg tgtacgtaca tttgcaaagt tcattgctga atatggggaa gaaaaattta
     4741 tggatgctat agagaaaaac gaattagatg gaatagtata ccattatgaa ggtcaaatta
     4801 taggtgatta tgataatact caaggtgagg atgagataag acaacttctt ttgcggggat
     4861 taagaatata attttattca atacttaaga cgcaatcttc tttataaata tacctccaaa
     4921 attaatggta ttcatagtaa acaacgtata caaaacaccg tgaactacat cgtcattaaa
     4981 atgacgagct tctggttccc tgaaaacagg tacacctacg gcgtgtccaa caccactact
     5041 gctgggtagg tgttatccta ctctacacag gtactttggg ctcattacct tgaatatttt
     5101 acgcagcaaa ggattacttg ctgcaacccc atatattcag ttatcaaggt acgacttttg
     5161 acctctgatt atattgtatc agaacatttg ttttcaggca atgcatttta tagaagtgat
     5221 gtaaagtttc gtatcgaaac cacagcttcc atctgcctca ttcaaatgag gaggattccc
     5281 gctgcgtttc ctaaa
//
LOCUS       DAOSDD010000279         5279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5558_length_5279_cov_6.160605, whole genome shotgun sequence.
ACCESSION   DAOSDD010000279 DAOSDD010000000
VERSION     DAOSDD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_5279_cov_6.160605"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5279
                     /locus_tag="PK733_17415"
     CDS             <1..>5279
                     /locus_tag="PK733_17415"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ12346.1"
                     /translation="YGRITYKATLPEGVSQYYVQFIYEDEYDGVLVKKEVRRILKNAI
                     GKVELSGYVKNNFKDTLPNAQVIIGTKSVFTDDTGFYKISGLDVGSYQISVTANNHYD
                     FQDTVTLSQKSTVLNMSLTRRAAHGNPIIRYILLNDTLPSRNENIIFYKGIDMDLNLF
                     ALIDWKGHDPGYVQFNVNGRIYNKKDARISLKLSELEEGGKLTVTCVTKSGEKSPEYN
                     SGIYVVDSLPDQFPATFVPTYIDGKYVVDQYIKLPEIKTPNIANIPLFAGGPFTFFAE
                     PALLTGAMSHDGSFHAFIGHSTDTNVGLAYTKNNKLQKYIMEKYATTEEQKENIKKGR
                     SRSFVATASMDSTLDASFYWNYDVKTSRWVFKYTEMMLGYNANMYAKYSWGVPGVPLT
                     AYLSANVDVSLNSHLYGYYYTPTTLSYTSGIVDLSKFRITMSGGLDAMIASAGVNFSG
                     EGKVKISYPVYKRDYYIGVRGWVNLKALIWAVDLPFLEYEWGDPSPWNLKKSSMRMNT
                     LSEDSTIMGIAEEGIDNLKPLARDYVNTQSSWLSGTENRLFSASNTNNAGIGTYSSQD
                     GSSALITGTIPYTDHKVIPYGDGAFMVFTEDDPARSDLDRTRAVYTVYKGNSWSQPLP
                     IDPLDQTGDYKPKIAATNKGVIAAWENISEVLPGDAELGDALEKEEIVVAVFDRETGN
                     WSDIRNLTTDRYMDVSPEIAADGDKGILVWVKSKAIDYSDALTADFAPENHIMFSSYD
                     GHTFSEPVSIYQTDSLIVNSSLAYEGDMAAYVFSLDMDKDLSTDADQEIFIMTYNGSE
                     WSAPGRITEDNLKDTKPRVTFLNGKPFLVWHRDGHIVYTRNALANPVVPKLAIEDTKL
                     QENFTLAARNKNNESIILTAPGVSEESQEIYALVYDTTNDVWSNELKLTDDMAINRMP
                     SAAVAGNTLITLYNRAHLEDVINPETGEIFKYPGNSADLMMNTKGLVHDLEITSDGII
                     LSESNVYPGGLVTISALVENKGIFGETGVNVAFYNGDPSNGGKQIGGTKVIEDVIVPG
                     GRGIASVDWDVPVEGLLDEIYVAVDPNSSINDINPLNNKANIKLKQPDIELSLLEAEL
                     MGSNNYAVKVKVSNIGGVDLPDSTITFYRGTGEGEIEEIGNINTGELLAGDVKEIPHL
                     LNLPSTQFINNSLDLIATVDAPSEYTEQDLANNVAMLTIKEEYPIIQSYEPGDNSVNV
                     RVDKEFRIIFDREIYQGDTFGDIALKDSGNNIIPATKAINEDTLIVTPSQNLKYNSEY
                     ILILPAMSIAGSNMHGMAEDWSISFKTEENKAYPLITALDGITPGQVISIPFSSDIVR
                     DANFNLITMKKVNNGDKIVAIYTITGNVLEIEPLSGLEYNTSYTVAVPEGAMKDKGGR
                     SINSFDFTLTTLEQGPDDEDVFTIIFDKNGGDTEASPAAKKVRSGANAGTLPAAPTRS
                     GYTFSSWNTKANGSGTAFTAATVVAGNITVYAQWTLNNSGGGVIPPGGNGSGTDPDTT
                     QPAEKDTTKPDTAEPDAIEPDTTKPDTTKPEIPKTEINVRGNTIIATTTVAVDVDNEG
                     NGVALVTQEQINDAIDKVLERAGKQEKGTMNGTTMTEIEVRVEAPSDIRTVETNIPKE
                     AVNQSAKSGISAVTVSTPVAYMTFNASALSAIYEKASSDVKITVSKVEASSLSVEVRK
                     MVGDRPVFDFSVTSGGKTISQFGGNVTVSVPYTPQKGEDINAIVIYYINEDGKLETVK
                     DCKYHPDTGRVIFNTDHFSLYMVGYNKVSFKDV"
BASE COUNT         1764 a         1033 c         1208 g         1274 t
ORIGIN      
        1 gctacggcag aataacttat aaagctactc ttccagaagg agtatctcaa tactatgttc
       61 aatttattta tgaggatgag tatgacggtg ttctcgtgaa gaaggaggta agaagaatcc
      121 ttaaaaatgc cataggaaag gttgagttgt ccggctatgt aaagaacaat tttaaagata
      181 ctcttcccaa cgctcaagtg attattggaa caaaatccgt ttttactgat gatacaggct
      241 tttataaaat atccgggtta gatgtgggtt cataccaaat atccgttaca gccaataatc
      301 attatgattt tcaggatacc gttaccctgt cccagaagag tactgttttg aacatgtctt
      361 taacaagaag agcagctcat ggtaatccca tcataagata cattttattg aatgacactt
      421 tgcccagcag gaacgagaat ataatcttct ataaaggcat agatatggac ttgaatctct
      481 ttgcgttgat agactggaaa ggccacgacc ctggatatgt acaatttaac gtaaacggaa
      541 ggatctataa caaaaaggat gcaaggatat cattaaaatt aagcgaattg gaagaaggtg
      601 gcaagctgac cgtaacctgt gtcactaaat caggagaaaa atctcccgaa tacaacagcg
      661 gaatctacgt ggtagactca cttccggacc aattccccgc tacattcgtt cctacatata
      721 tagacgggaa atatgttgta gatcagtata tcaagcttcc cgaaattaaa actccaaaca
      781 tagcaaacat acctttattt gcaggaggtc cctttacttt cttcgcagag cccgccctgt
      841 taaccggagc catgagccat gacggatctt ttcacgcctt cataggtcat agtacggaca
      901 caaacgtcgg tcttgcatat acgaaaaaca ataaactgca aaaatacatt atggaaaaat
      961 acgcaactac cgaagaacaa aaagagaata taaagaaagg aagatcgaga tcctttgttg
     1021 ccacagcttc aatggattct acgctggatg cttccttcta ctggaattac gatgtcaaaa
     1081 caagcagatg ggtatttaaa tatacggaga tgatgcttgg atataatgca aatatgtatg
     1141 caaaatacag ctggggagta cccggtgtac ctttgaccgc ctatctatct gcaaatgtag
     1201 atgtaagttt gaattctcat ctatacggat actactatac cccaactaca ttaagctaca
     1261 cctcgggaat cgtggatctg agcaaattta gaataaccat gtccggagga cttgatgcga
     1321 tgatagcttc ggcaggggtc aacttctcgg gagaaggaaa ggtcaagata tcataccctg
     1381 tgtataaaag agactattat atcggcgtaa gaggatgggt aaacctgaaa gcccttatct
     1441 gggctgttga cctccccttc cttgaatacg aatggggaga tccttcaccc tggaatctta
     1501 aaaaatcttc catgaggatg aatactctta gtgaggactc caccataatg ggcattgcgg
     1561 aagagggaat agataatctg aagcccctgg cgagagatta tgttaacacc cagtcttcct
     1621 ggctttccgg cacagagaac agactatttt cagctagcaa tactaacaat gcaggaatag
     1681 gcacttacag ttcgcaggat ggaagcagtg cattgattac aggaaccata ccatatacgg
     1741 atcacaaggt aataccttat ggtgatgggg cattcatggt attcacggaa gacgatccgg
     1801 caagatcaga tcttgacaga acaagagcag tttataccgt ttataaagga aatagctggt
     1861 cacagcccct tccaattgat cctttggatc aaaccgggga ttataagcct aaaatagccg
     1921 cgacaaataa aggtgtaatc gcagcatggg aaaacataag cgaggtgctc ccgggagatg
     1981 cggagcttgg agacgcactg gaaaaggaag aaatagtggt tgctgtcttt gacagggaaa
     2041 cagggaactg gtcggatatc cggaacctaa ctaccgacag atatatggat gtatcccctg
     2101 aaatagcagc agacggagat aagggaatat tggtgtgggt aaagagcaag gccatagact
     2161 acagtgatgc tttaacagcg gattttgcac cggagaatca tatcatgttc tcctcgtatg
     2221 acggccatac ctttagtgag cctgtatcaa tatatcaaac cgacagcctc atagtgaact
     2281 catcccttgc atatgaaggg gatatggcag catatgtatt ctcactggat atggataagg
     2341 acttaagcac cgatgcagat caggaaatat ttatcatgac atataacgga agcgaatggt
     2401 ctgcacccgg aaggattaca gaggacaatc ttaaggatac aaagccccgg gtaaccttcc
     2461 ttaatggcaa gcctttcctg gtatggcaca gggatggtca tatcgtttat acaaggaatg
     2521 ccttggcgaa tcctgttgtg cctaaattgg caatagagga tacaaagctt caggaaaact
     2581 tcacacttgc agcaaggaat aaaaacaatg aaagcataat tctgacagca ccaggtgtat
     2641 ccgaggaaag tcaggaaatt tacgcacttg tttacgatac caccaatgat gtatggagca
     2701 atgagcttaa attaaccgat gatatggcta taaatcgtat gccatcagcc gcagtagcag
     2761 gtaatactct gataacctta tataacaggg cacacctgga agacgtgata aatccagaaa
     2821 caggagagat cttcaaatac ccgggaaaca gtgctgatct gatgatgaac acaaaggggc
     2881 ttgttcatga tctggaaata accagcgatg gtattatatt atccgaaagc aatgtatatc
     2941 cgggaggact tgtaaccata agtgctttag ttgaaaacaa gggaatattc ggggaaacag
     3001 gagtaaatgt agctttctat aacggagatc cttcaaatgg tggaaaacag atagggggaa
     3061 ctaaggtgat cgaagatgta attgttccag gaggaagggg gattgcatca gtggattggg
     3121 atgtacctgt tgaagggctc ctggacgaaa tatacgttgc ggtggaccct aacagcagca
     3181 taaacgatat taaccctctt aacaacaagg cgaatattaa gctgaaacag ccggatatag
     3241 agttgtctct tcttgaggca gaactcatgg gcagcaacaa ttatgcggta aaggttaagg
     3301 tatcgaatat aggaggcgtt gaccttccgg attctacaat caccttctat cgtggcacag
     3361 gtgagggcga gattgaggaa ataggcaata taaatacagg tgaactatta gcaggtgacg
     3421 taaaggagat accacacctt cttaaccttc cttcaaccca gtttataaac aactccctgg
     3481 acttaatagc cacagtggat gccccttcag aatatacgga gcaggatctt gcaaacaatg
     3541 tggcaatgct aaccattaag gaggaatacc ctattattca aagctatgag ccgggagata
     3601 attcagtgaa tgtaagagta gacaaggaat tcagaattat ctttgataga gagatatacc
     3661 agggagacac ctttggtgat atagctctta aggattccgg caataacata atacctgcaa
     3721 caaaggcaat taatgaagat acccttattg ttaccccttc tcaaaatctt aaatacaaca
     3781 gtgagtatat cctcatctta cctgccatga gcattgcagg cagcaatatg catgggatgg
     3841 cagaagattg gagcatctcc ttcaaaacgg aagaaaacaa ggcatatcca ttgattacag
     3901 cacttgatgg aataaccccg ggtcaggtaa taagtattcc tttcagtagt gatattgtca
     3961 gagatgcaaa ctttaaccta attactatga agaaagttaa taacggagat aaaattgtcg
     4021 ccatatatac tataaccgga aatgttttgg aaattgaacc tttaagtggt ctggagtaca
     4081 atacatccta tactgtagca gtaccggagg gagcaatgaa agataagggt ggaaggagta
     4141 tcaactcatt cgactttaca ctgacaaccc ttgagcaagg acctgatgat gaggatgtat
     4201 ttaccataat ctttgacaaa aatggcggtg ataccgaagc aagccctgct gcgaagaaag
     4261 taagatccgg tgctaatgca ggcacattac cggcagcccc cacaagatcg ggttatacct
     4321 tcagtagctg gaacaccaaa gcaaacggaa gcggaacagc ctttacggca gctacggtag
     4381 ttgccgggaa tataacagtg tatgcacaat ggaccttaaa caattcagga ggaggagtta
     4441 tcccgccagg aggcaacgga tccggcactg atccggatac aacccaacca gctgaaaagg
     4501 atacaaccaa accggatacg gctgaaccgg atgcaatcga accggataca actaaaccag
     4561 atacgaccaa gcctgaaata ccaaaaaccg agattaacgt cagaggaaat acaattattg
     4621 caactacaac agtagctgta gatgttgaca acgagggcaa tggagttgca ttagttacac
     4681 aggagcagat aaatgatgct attgacaaag tattggagcg agccggaaag caggaaaaag
     4741 gaacaatgaa cggaacaaca atgaccgaaa ttgaagtcag ggttgaggct ccatcggata
     4801 taaggacagt ggagacaaat attcctaaag aggctgtgaa ccagtctgct aaatccggta
     4861 tcagtgcagt gacggtatca acgcctgttg catatatgac ctttaatgca agtgccctct
     4921 ctgcaatata cgaaaaagca tcgagcgatg tgaaaataac cgtttctaaa gtggaagctt
     4981 cctctctttc agttgaagta cggaagatgg ttggagacag gccggtgttt gattttagtg
     5041 tcaccagcgg aggcaaaacc atatcgcagt ttggaggaaa tgttacagta tcagtgccct
     5101 acacgccgca aaaaggagag gatataaacg ctatagtcat ctactatatc aatgaagatg
     5161 gcaaactgga aacagtaaaa gattgtaaat atcatcctga cacaggaaga gtcattttta
     5221 acacagatca cttctccctg tatatggtag gttataataa ggtaagcttc aaggatgtg
//
LOCUS       DAOSDD010000280         5250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5602_length_5250_cov_5.111453, whole genome shotgun sequence.
ACCESSION   DAOSDD010000280 DAOSDD010000000
VERSION     DAOSDD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5602_length_5250_cov_5.111453"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="hypE"
                     /locus_tag="PK733_17420"
     CDS             <1..862
                     /gene="hypE"
                     /locus_tag="PK733_17420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOJ12347.1"
                     /translation="KIAVTTDSYVIKPLFFPGGDIGRLCISGTVNDLAVSGACPKYIS
                     AGMIIEAGFDMEMLVRIVESMAMTAKEAGVCIVTGDTKVVEGGSADGIFINTAGIGVF
                     SKERPPLPQKIQQGDKIIISGYMASHGMAIMAARENMGFTPSIKSDVAPIAALADAIL
                     KKGYKVNAMRDPTRGGVAATLCEWVAPDTDIAIYDKSLPVRPDVRAACEILGMDPLYI
                     ANEGIMLLSVPENYADSVLMSLHSVDLGKDAVIIGQVTKGCGYVYAVTEYGTSRKVMK
                     PRGELLPRIC"
     gene            1173..1649
                     /locus_tag="PK733_17425"
     CDS             1173..1649
                     /locus_tag="PK733_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12348.1"
                     /translation="MNTKLRNIATLVFIAIAFICAVLTLPDLLAGSALYLHYELPLGL
                     DFGFYADALAVFMAMTASLVASVIVLYSFGYIKEYKNQNEYYMMVCLFIGAMMGLVFS
                     TNLIFIYFFWEISAICCWRLIGFLSGKNNHMSGQQGVYHDRCRCPYYAPEEKGTAL"
     gene            1744..2667
                     /locus_tag="PK733_17430"
     CDS             1744..2667
                     /locus_tag="PK733_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12349.1"
                     /translation="MARKERKKSPEYTYHVMTRSITEIDLFRCDEDKDYYLNLLKRYK
                     EKYHCSIYSYILMNNHTHVYINPCGADISTFMLCLNTAYVSYYNKRYKRRGHLFQDRF
                     ASTVVDNNTYSFKLTAYINNNAKDLPGYRGKEELYPYSSYGIYTGFREDYYELVDTEF
                     LLKQFSIDKNIARERYRAFTESMKDTDAAKEVDENIMRAYTENVYRSEKSYIVRTEEP
                     DKILVKIGQILGENLTEGLRLKHSRENSKNRAFVTYILRTLCGYTYKQLCKYIGNMSM
                     SGVSRLSNEGFRIFKEQVIYRNAFSALIRTT"
     gene            2947..3873
                     /locus_tag="PK733_17435"
     CDS             2947..3873
                     /locus_tag="PK733_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOJ12350.1"
                     /translation="MNILHLKYAVEVEKTGSINKAAKNLLMGQPNLSRAIRELEESLG
                     VTLFKRTSKGMSPTPQGEEFLQYARKILAQIDEVEAMYRNRNEDKQMFSISVPRASYI
                     ACAFTEFAKSIDTKKQAEILYNETNSMRAISNILQADYKLGIIRYQTTYEEYFKALLH
                     EKGLSHEVIVEFSYQVVMSKHHPLANKSIIKLSYLSEYIEIAHADPYVPLMPQLDAKK
                     AELSEFVDKRIFVFERGSQFDLLSSVTSTFMWVSPIPQRLLDQYGLVQKTCPENKKKY
                     KDVLVYRKGYHFTELDKKFICKLDDFKVVFKK"
     gene            4221..5009
                     /locus_tag="PK733_17440"
     CDS             4221..5009
                     /locus_tag="PK733_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOJ12351.1"
                     /translation="MIFSYYPGCTLKTKAKDLDEYARRSAEALGIVLEELPDWQCCGG
                     VYPMAQDEIATKLSSIRALVSAKSFERNLVTLCSACHHVIKRVNNDMKTNESIRTKVN
                     NYLKLDIDYAGETKVIHYLEMLRDVVGFDQLKGKVVNPLTGRKIGAYYGCLLLRPSKT
                     MQFDNPENPTIIEDFISAIGGTPVAYPYRNECCGGYVTLTKNNLAQEKCNNIMASASE
                     KGAEAMITACPLCMYNLDTNNSTEQKLPVYYFTELLAEALGVKL"
     gene            5026..>5250
                     /locus_tag="PK733_17445"
     CDS             5026..>5250
                     /locus_tag="PK733_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ12352.1"
                     /translation="MNTVNKVNTVNTAKKEIGFQRPLGNSIKTLSVDVEKRELIDRIL
                     RISGVNTRKCMKCGKCSAACPAFNEMDIHPH"
BASE COUNT         1773 a          836 c         1126 g         1515 t
ORIGIN      
        1 aaaaattgca gtgaccacgg acagctacgt tataaagcca ttgttctttc cgggaggcga
       61 tattggaaga ctgtgtataa gtggtacagt aaacgacctg gctgtcagcg gtgcatgccc
      121 taaatatata tcagcaggta tgatcattga agcggggttt gatatggaga tgcttgttcg
      181 tattgttgaa tctatggcaa tgacggcaaa agaagcaggt gtgtgcattg tgaccgggga
      241 cacgaaagtg gttgaagggg gctctgcgga cggtattttt ataaatactg caggtattgg
      301 cgtgttttca aaggaaagac caccccttcc tcagaaaatc caacagggag acaagataat
      361 cataagcgga tatatggcgt cacatgggat ggccatcatg gcggcacgtg aaaatatggg
      421 ttttacccca tccataaaaa gtgatgttgc tcctattgcc gctctcgcag atgcgatact
      481 aaaaaaaggt tacaaagtca atgccatgcg tgatcctacc cgtggcggtg ttgctgcaac
      541 tctttgtgaa tgggttgccc cggataccga tattgccata tacgacaaat cacttcctgt
      601 acgacctgac gtacgggctg catgtgagat attgggcatg gatccgctgt atatagccaa
      661 cgagggcatt atgcttttat ccgtgcctga aaactatgcg gatagtgtgc ttatgtctct
      721 gcattctgtc gacttgggta aagatgccgt gataatagga caagtaacaa aggggtgtgg
      781 ttatgtatat gctgttactg aatatggcac aagtagaaag gtaatgaagc caagagggga
      841 attgctcccg agaatttgtt gatgtgaaaa tcaaattatg acatattttg tattttacag
      901 taaagttttc tcgtaaaatt tatttgttga ttacttgact atataattac tttatattac
      961 aatttggtat ggtggatggg cagactgaca cataatcagc acaaaactgg aaagtgtgta
     1021 aagacatgcc aatattagtt gcgcagagtt taaataatac ccatctgtat attggactat
     1081 taaggtggac taaaaattac gaataattgt ataattatta tatgtatttc tgtaccattg
     1141 cttggtgctt ttttctccca attttaggta atatgaatac taaattaagg aatatagcaa
     1201 cattagtttt tattgctatt gcctttattt gtgcagttct tactttgccc gacttgcttg
     1261 caggctctgc tctatatctg cattatgagc tgcctctggg attggatttt ggtttttatg
     1321 cagatgccct tgctgtattt atggcaatga ctgcgtctct tgtagcatct gttattgtcc
     1381 tttattcatt tggatatata aaggaatata aaaaccaaaa tgaatattac atgatggttt
     1441 gcctgttcat aggtgctatg atgggactgg tcttttccac aaacctgata tttatatatt
     1501 ttttttggga gatatctgca atttgctgct ggaggctcat aggtttttta tcgggaaaaa
     1561 ataaccatat gtcgggccaa caaggcgttt atcatgaccg ttgtcggtgc ccttattatg
     1621 ctcctgaaga aaaggggaca gccctgtaga aagagccctg tagaaagaat cctgtagtaa
     1681 gaaaagaaag aatattgaca aaaatttcat tatatggtaa cataaaccct gaggtgataa
     1741 aaaatggcaa gaaaggaaag aaaaaaatct ccagagtata cgtaccatgt gatgacgagg
     1801 agcatcactg aaatagacct gtttcgatgt gatgaggata aagattatta tttgaacctc
     1861 ttgaaacgat acaaagaaaa atatcattgc agtatttatt cctatatact tatgaataac
     1921 catacacatg tatatattaa tccttgcgga gcagatattt caacttttat gttatgcctt
     1981 aatactgctt atgtttccta ttacaacaaa agatacaagc gacgtgggca tctgtttcaa
     2041 gacaggttcg ctagtaccgt agtggacaac aatacttaca gctttaagct tacagcatat
     2101 attaataaca atgcaaagga cttaccaggc tataggggaa aagaggagtt atatccatat
     2161 tcatcttatg gaatatatac cgggttcagg gaggattatt atgagcttgt ggatacagaa
     2221 ttcctgttaa agcagtttag tattgataaa aatatagcgc gggaaagata tagagctttt
     2281 actgagtcaa tgaaggatac agacgctgca aaggaagtgg atgagaatat tatgagggct
     2341 tatactgaaa atgtgtatag aagtgagaaa agttatattg taaggactga ggagcctgat
     2401 aaaatattgg ttaaaattgg gcagattctg ggcgaaaatc tcacagaagg attaaggttg
     2461 aagcacagca gagaaaacag caaaaacagg gcatttgtaa catacatatt gaggacctta
     2521 tgtggatata catacaaaca actatgtaag tatataggta atatgtcaat gtcaggagta
     2581 tcaagattgt caaatgaagg ttttaggata tttaaagaac aggtaatata ccggaatgcc
     2641 tttagtgcct tgattcggac tacataacat aaattcgaac ccaataacat gagttcaggc
     2701 tatgtaacat caattcgagc aacaaaaaga aatgagggga cagtcctgca gaataagcag
     2761 aaagagggac agaaagaggg acagtcctgt atatacagga ctgtatatac acctgaacaa
     2821 taatatccaa ctttacatta gaacaaaata ctattataat aagtcaggaa aaatgaatgg
     2881 ctatgtagag gctatttgtg taaatgtgta aagtctgttt tgtgtaaagg ggatggatta
     2941 tacgctatga atatattaca tttaaaatat gctgtagagg tggagaaaac cggttctatc
     3001 aacaaagctg ccaaaaattt attaatggga caaccgaatt tgagtcgtgc aattagagaa
     3061 ttggaggagt cccttggtgt taccttattt aagcgtactt ctaaaggaat gtccccaaca
     3121 ccccaaggag aggaattcct gcaatatgca agaaaaattc ttgctcagat tgatgaagta
     3181 gaagccatgt atagaaatag aaatgaggat aagcaaatgt tttccatttc tgttcctcgt
     3241 gcaagctata ttgcttgtgc gtttacggaa tttgccaaaa gcattgatac taaaaaacaa
     3301 gcggaaatat tatataatga aactaattct atgcgtgcaa taagcaatat tctgcaggca
     3361 gattataagc ttggcataat tcgctatcaa acaacctatg aagagtattt taaagctctg
     3421 ctgcatgaaa aaggtttgtc acatgaagta atcgtggaat tctcttatca agttgtcatg
     3481 tcaaagcatc acccgcttgc aaataagagc atcattaaac tgtcatattt gagcgaatat
     3541 atagaaattg cccatgcgga tccttatgta ccgttaatgc ctcagttgga tgcgaaaaaa
     3601 gcggagcttt ccgagtttgt ggataagcgc atttttgttt ttgaaagagg cagtcaattt
     3661 gacttattga gcagtgttac gagcacattt atgtgggtat cacccattcc acagcgtttg
     3721 cttgaccaat acggcctggt tcagaagaca tgccctgaaa ataagaaaaa atataaggat
     3781 gtattggttt atagaaaagg ttatcatttt actgagctgg acaaaaaatt catttgcaag
     3841 ctagatgatt tcaaagtagt attcaaaaaa taacataaat tatataattt ataccaatat
     3901 gatttatacc aaaatgattt ataccaaaat gatttatacc aaaatgattt atactaatat
     3961 gatttatact aatatgattt acattaatat gatttatact aaaaactcat tcaaaattac
     4021 catttaggaa gaaaatacgc ttgaatcaac agacttaagt caagggtata tccataaaaa
     4081 catagcggta tgcctttttt atatttcaca aaatgcaaaa agattgttaa aataataaca
     4141 tagttagttt gttaaaataa taacaataaa aaacaacaat aaaaatttgt atatcaaaat
     4201 atagtgtgag ggaatttgat atgattttta gctactatcc agggtgtacc ctgaaaacaa
     4261 aagcgaagga cttagatgaa tatgcgagga gatctgcaga agcacttggt atcgtgcttg
     4321 aggaactgcc agactggcag tgttgcggtg gtgtttatcc aatggcacag gatgaaatcg
     4381 caacaaaact ttcgtctatt cgtgctcttg tatcagcaaa aagctttgaa agaaatttag
     4441 taacactgtg ctctgcttgt catcatgtaa tcaaaagggt caataacgac atgaagacaa
     4501 acgagagtat tagaacaaag gtcaacaatt acttaaaact agatatagat tatgcagggg
     4561 aaacaaaggt aatccactac ctggagatgc ttcgtgatgt cgttggattt gatcaactta
     4621 agggaaaggt cgtgaacccc ttgacgggca ggaagattgg ggcgtattac ggatgccttt
     4681 tactccgtcc cagcaagacg atgcagtttg ataacccaga aaacccaaca ataatagagg
     4741 attttattag tgcaattggt gggaccccgg ttgcttatcc gtaccgtaat gaatgctgcg
     4801 gcggttatgt aacacttacc aagaacaatc ttgcacagga aaagtgcaac aatatcatgg
     4861 catcagcttc tgagaaaggc gcagaagcca tgattactgc atgtccgctt tgcatgtaca
     4921 atctggatac aaacaacagt acagaacaaa aactgccggt atattatttc actgagctat
     4981 tggcagaggc acttggtgtg aagttataaa caggagggat tatcagtgaa tacagtgaat
     5041 aaggtgaata cggtgaacac agcgaaaaaa gaaattggat ttcaaagacc ccttggtaat
     5101 agcatcaaga cattatcagt tgatgtagaa aaaagagagt tgatcgatag gatactaaga
     5161 ataagcggag tgaataccag aaagtgtatg aaatgcggaa aatgctctgc tgcatgtcct
     5221 gcatttaacg agatggatat tcatccccat
//
LOCUS       DAOSDD010000281         5235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5618_length_5235_cov_5.076641, whole genome shotgun sequence.
ACCESSION   DAOSDD010000281 DAOSDD010000000
VERSION     DAOSDD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_5235_cov_5.076641"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PK733_17450"
     CDS             <1..329
                     /locus_tag="PK733_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12353.1"
                     /translation="IVAILILNFLTADKLRWDGRPYSEASRLANYYQGSAMSPGVAKE
                     MRVYDLAKETIENWDKAYDKEALTSTANNMTIRPAVQLYKGLACSFKIFTYFMLHSEK
                     MSDLVI"
     gene            263..793
                     /locus_tag="PK733_17455"
     CDS             263..793
                     /locus_tag="PK733_17455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12354.1"
                     /translation="MFFQDFHIFHATLRENVGFGDLKNIGNIDRIQIAMQKGGADKLP
                     ERFPKGLEQWLLRNVIKDGAMLSGGEKQRVAVSRAHMSDKEILIFDEPAAALDPIAEM
                     KQFHTIREKIEGRTAILISHRVGFARLADRIIVLDGGRLVENGTHDELMALNGIYASF
                     FKEQAQWYTTEEGEAV"
     gene            790..2574
                     /locus_tag="PK733_17460"
     CDS             790..2574
                     /locus_tag="PK733_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12355.1"
                     /translation="MSKLRTLLKALIVSVKIKGAFSMVISLLGFAVAFFPVIIAQQLR
                     ALTDSLQSLVGTGGSAGPSLVIFTGVVGLYMVQVIITNIQQYTNGLDEVKIQRYIKRT
                     IMRHKCEVRYKYIENYDDFQKRLTFIEEYAGYHMAKCIGNMITILQLIIAFIVASLAL
                     WKINPYIVLILFVTSIPAALLSYFQQDETFRYRAKWTEEGALAIHYFATIGGAGYSYA
                     GLQEIRHFGLFDYLKARWRSIADEYISKKNKIMYKHVKFNTAADFLRSVVYLGILLIA
                     AWSIYDNPAIGLGAFTLVFALSGQLQTSTGDCLVSIMVLAQNIPYMKEFFYLDDIERE
                     PQVGKTTCAADGNIEFNDVSFAYPNTDVDVIKNITVQIKEGEKVAIVGENGSGKSTFI
                     SLLCGMFDSKSGRISVGGIDIKSNPVAARNTVSVVFQDFARYESSIRENITVSDKNRM
                     ATDEEIMELLRKINVDDVVKEQKNGLHELVGSFSEKANNLSGGQWQKISIARAAYRNK
                     AKVMILDEPTSALDPLAEAQLYRNFASLTEDKTTILISHRLGITAIVDRILVFKDGRI
                     VEDGSHKQLMAMNGHYAEMYRAQAQWYA"
     gene            2619..2768
                     /locus_tag="PK733_17465"
     CDS             2619..2768
                     /locus_tag="PK733_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12356.1"
                     /translation="MIQLLENDEEKLIETASKYNNEAYYAEYEKVLKTYLNINRDEYK
                     EFLRL"
     gene            complement(3162..3953)
                     /locus_tag="PK733_17470"
     CDS             complement(3162..3953)
                     /locus_tag="PK733_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOJ12357.1"
                     /translation="MQVTTTEVQNNFGKYLKLCKYEDIIVTRNGKKIARLTTYNDNID
                     NYFIREAAEKFAYSGKKISYEEFLEISQNSELRYEYIDGEIFLLASPFYEHQKAIGQI
                     FGEFVFWFKDKNCEPLTSPFDVTLFKKNSNEDINTNIQDANNEDEADTEEYKKNINIV
                     QPDILVICDKENIDEKGRYHGTPSLVVEVLSQSTKKKDLAKKLDLYSSSGVKEYWVVN
                     TFSKEVYIYAFKDLNIEKMITFKSGDKVESVIFNGLEINVDQVFA"
     gene            4238..4486
                     /locus_tag="PK733_17475"
                     /pseudo
     CDS             4238..4486
                     /locus_tag="PK733_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     misc_binding    4556..4787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4842..>5235
                     /locus_tag="PK733_17480"
     CDS             4842..>5235
                     /locus_tag="PK733_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ12358.1"
                     /translation="MSFKTYDPQIIESKWRKRWEENHAHETGDALVKPKFYCLDMFPY
                     PSGDGLHVGHWRGYVLSDVWSRYKKLQGFNVLHPMGWDAFGLPAENDAIKKGIHPQVS
                     TQRNIGNFKRQLTEMGAMYDWDREVNTSD"
BASE COUNT         1681 a          882 c         1064 g         1608 t
ORIGIN      
        1 atattgttgc catcttgatc cttaactttt tgacagctga taagctccgt tgggacggcc
       61 ggccttattc agaggcttca cgtcttgcaa actattatca aggctctgct atgtcaccag
      121 gcgtggctaa ggaaatgcgt gtttatgatt tagctaaaga aacaatagaa aattgggata
      181 aagcatacga taaggaagcc ttaacttcta cggcaaacaa tatgacaata agacccgcgg
      241 tgcagttata caaagggttg gcatgttctt tcaagatttt cacatatttc atgctacact
      301 cagagaaaat gtcggatttg gtgatttgaa aaatattgga aacatcgacc gtattcagat
      361 cgccatgcaa aaaggaggag ccgataagct gccggaacgc tttcctaaag gccttgaaca
      421 atggctgctg cgtaatgtta tcaaggacgg agccatgtta tccggtggag aaaaacaacg
      481 ggtagcagtt tcccgggcac atatgagcga taaggagatt ttaatatttg acgaacctgc
      541 cgctgcgctt gatccaatag ctgagatgaa acaattccat accattcgcg aaaaaattga
      601 aggccgcacg gcaattctca tcagtcatcg tgtgggtttt gcgcggcttg ctgacagaat
      661 tatcgtattg gatggcggaa ggcttgtgga aaacggcacg catgatgaat taatggcgtt
      721 gaacggaata tatgccagct tttttaaaga acaggcgcaa tggtatacaa cggaagaagg
      781 tgaagctgta tgagcaaatt acgaacactg ttaaaagcac ttatagtaag tgttaagatt
      841 aaaggtgctt tttctatggt cattagcctg cttggttttg ctgtggcgtt ttttccggta
      901 atcatagctc aacaacttag agcattgaca gattcacttc aatcattggt tggaaccggg
      961 ggaagcgcag gtccttcgct tgttattttt acaggtgttg ttggacttta tatggtccag
     1021 gtaatcatta cgaatattca gcagtataca aatggacttg atgaggtgaa aatccagaga
     1081 tacatcaaac gcacaattat gcgccacaaa tgcgaagtcc gttataagta cattgaaaac
     1141 tatgatgatt ttcaaaagcg tcttactttt atcgaagaat atgcaggtta tcatatggcc
     1201 aagtgcatcg gtaatatgat aacaattcta cagctcataa ttgcatttat tgttgcgtca
     1261 cttgcgctgt ggaagataaa tccctatatc gtactgattc tctttgtcac aagtatacct
     1321 gccgctttat tatcatattt tcagcaagac gaaacttttc ggtatagggc aaagtggacg
     1381 gaagaagggg ctcttgccat acattatttc gcaacaatag gtggagctgg atattcatat
     1441 gctggcctgc aagaaatccg tcattttgga ttgtttgatt atttgaaagc acgttggcgg
     1501 tctattgcgg atgagtatat cagcaagaaa aacaaaataa tgtataagca tgttaagttc
     1561 aacaccgctg ccgattttct gagaagtgtt gtgtatttgg gtattctcct aattgcagct
     1621 tggtctatat acgataaccc ggctatcggc cttggagctt tcacgcttgt atttgctctt
     1681 tcaggacaat tacagacctc aacaggagat tgtcttgtca gcattatggt cttggcacaa
     1741 aacattccat atatgaagga attcttttat ttagacgaca tcgaacgtga accgcaagtc
     1801 ggcaaaacaa cttgtgctgc ggatggaaac attgaattta atgatgtgtc ttttgcttat
     1861 cccaatactg atgtcgatgt aataaagaac attacagttc aaattaagga aggtgagaaa
     1921 gttgccattg tgggcgagaa cggcagcgga aagtcaacct ttattagtct tttatgcggt
     1981 atgtttgatt ctaaatcagg acggattagt gtaggcggaa ttgatataaa gtctaaccct
     2041 gtggcggctc gaaacactgt gtcagtggta ttccaagact ttgcccgtta tgagtccagc
     2101 atccgggaaa atatcaccgt atcagataag aataggatgg ctacggatga agaaattatg
     2161 gaactgctac gcaaaataaa tgttgatgat gttgtaaagg aacaaaagaa tgggttgcat
     2221 gaacttgttg gctctttttc agaaaaagct aacaaccttt caggcggtca atggcagaaa
     2281 atctctatag cccgtgcggc atacagaaac aaggcgaaag tgatgatact tgatgagcct
     2341 acatcagctc ttgacccgct ggcagaggct caactatatc gcaacttcgc gtcgttgaca
     2401 gaagataaga caactattct tatttcacat cgccttggta tcacagctat tgttgaccgt
     2461 attcttgtgt tcaaggacgg cagaattgtt gaagacggct cccacaagca gctcatggca
     2521 atgaatgggc attacgcgga aatgtatcga gctcaggctc agtggtatgc atgagaataa
     2581 aacacctcta ctatgggaga gggatagatt accttttaat gattcaatta ctggaaaatg
     2641 atgaagagaa gttgattgaa actgcttcta aatataataa tgaagcttac tatgcggaat
     2701 acgaaaaagt attgaaaacg tatttaaata ttaatagaga tgaatataag gaatttttaa
     2761 gactataaga caagaagata ttaaagaagc agaagtattt ttagaaaaca aggaagatga
     2821 tttggcatta ctctacaagg gtgtaatgct ccttatgcaa aaatcaggac tctatctcac
     2881 agtctcaata gcctgtacat ttatatttta tgtgaccagc ttaattaggt gttttcaaaa
     2941 aattcattat tcaaaatttc tggctattaa gtgctcttaa aatagtaaca tacgattttt
     3001 cttgacaaaa tattagtgat aaactccaaa tatttatatt ggctcttatt aactttagtt
     3061 ttcgtgtcaa tagcagtcgt agtttaatat tttaataact ctaaggtatt aaatcaactt
     3121 ttttgaaaaa ccaattgacc agtttatttt ttgattatgt cttaagcaaa aacctggtca
     3181 acattaatct caagtccgtt gaaaataact gattcaactt tatctccact tttaaaggta
     3241 atcatttttt caatattcaa atctttaaag gcataaatgt acacttcttt agaaaatgta
     3301 tttacaaccc aatactcttt tacaccgctt gatgagtata ggtcaagttt ttttgctaaa
     3361 tctttctttt tagtagattg ggaaagaact tcaacaacaa gcgaaggtgt accgtgatat
     3421 cttccctttt catctatatt ttctttatcg caaattacta aaatatcagg ttggacgata
     3481 ttaatattct ttttatactc ttcagtatct gcttcgtctt cattattggc atcttgtatg
     3541 tttgtattaa tatcttcatt gctatttttt ttaaataatg taacatcaaa aggtgaggta
     3601 agaggctcac agtttttatc tttgaaccaa aagacaaatt cgccgaaaat ttgtcctata
     3661 gctttttgat gttcataaaa aggtgatgcc agcaagaata tttcaccatc aatatattca
     3721 tatcttaact cgctgttttg cgatatttcc agaaattctt catatgaaat ttttttgcct
     3781 gaataagcaa acttttcagc agcctctcta ataaagtaat tgtctatgtt gtcattataa
     3841 gtagtaagcc ttgcaatttt ctttccattt ctggttacta taatatcttc gtatttacat
     3901 aacttaaggt acttgccgaa attattctgc acttctgttg ttgtaacttg cataaaagca
     3961 cctccatatc agctaatata aatatatttt acctaatata ttggatttgg tcaagaattt
     4021 atctaaatat tttaactaaa taatattaac aattagctag actaataatc ttagaaagtg
     4081 catagcaata accaagttgg tatgaagata ccggtgcagg tgaaaaaaat tgtacgtgcg
     4141 gctaaaatga atttaagccc ataaaaactt tttcataaaa aagttttgtt tctgcaaggg
     4201 cggattcatt tcgcacaatg caggaatgga gcttaaaatg atattatcag gacttgaaat
     4261 taagaacaaa cttggaaaag atataataat tgaaccattt gatgaaaaac ttttgaatcc
     4321 caatagttat aatctgagac tacacaatga gcttcttgta tatgaagaag atgtcctcga
     4381 tatgaagaag ctaaataaaa caaggaatat aataattcct gaagaaggat atatacttaa
     4441 tcctggaaag ctttacctgg gaaggacttt tgaatataca aagacagttt ataaaataaa
     4501 tttttaattt ttattgactt aaagcatata atattataaa atcaatagca ataaaaaaaa
     4561 ggcaatgatt gggaatagta aaaggtgtca gaagctttca gagagccgag gttggtgtga
     4621 ttcggcagtt tcattccttt gaactcacct atgagctgca gcccgataat gtagggtgtg
     4681 ccggaaatcc tccgttaaaa ggatagatgg tgatggccat cgaatgagtg gattctttac
     4741 attaagaatc aattagagtg gtaccgcgaa ccagcctcgt ctctatacgt acagagatga
     4801 ggctttttta ttttagacaa aaaaagagcg aggtgttaag tatgagtttt aagacttacg
     4861 atccgcaaat tatcgaaagt aaatggcgta aaaggtggga agaaaaccat gcccacgaaa
     4921 caggagatgc cctggtaaaa ccgaaattct actgccttga catgttcccg tacccgtccg
     4981 gagatgggct tcatgtgggt cattggcgcg ggtatgtact gagtgatgtc tggagtagat
     5041 ataaaaagct tcagggcttt aatgtacttc atccaatggg atgggatgct tttgggttgc
     5101 ctgctgaaaa tgatgcaatt aagaaaggca ttcatccaca agtatctaca caaaggaata
     5161 tcggcaactt taagcggcag cttacagaaa tgggtgcaat gtatgattgg gatagagaag
     5221 ttaatacaag tgatc
//
LOCUS       DAOSDD010000282         5231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5624_length_5231_cov_4.988988, whole genome shotgun sequence.
ACCESSION   DAOSDD010000282 DAOSDD010000000
VERSION     DAOSDD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5624_length_5231_cov_4.988988"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..959)
                     /gene="cysK"
                     /locus_tag="PK733_17485"
     CDS             complement(24..959)
                     /gene="cysK"
                     /locus_tag="PK733_17485"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ12359.1"
                     /translation="MKKVSKSITELIGNTPLLETVNYNSQNGIKGKIIVKLEYFNPGG
                     SVKDRVGYAMIKDAEEKGLVNRDTVIIEPTSGNTGIALALVAAARGYRLILTMPETMS
                     IERRSLLAALGAELVLTPGSDGMKGAIKKAEELVSEIPNAFIPQQFNNPANSAAHRNT
                     TAEEIWRDTEGQVDIFVAGVGTGGTVTGVGEKLKEKNPDIRIIAVEPYDSPVLSGGTP
                     GPHKLQGIGAGFVPNVLNTSIIDEIVKVKSEEAFAAARKLAAAEGILVGISSGAALHA
                     ATEAAKRPDSAGKNIVVLLPDTGERYLSTALFQNI"
     gene            complement(1022..1468)
                     /locus_tag="PK733_17490"
     CDS             complement(1022..1468)
                     /locus_tag="PK733_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ12360.1"
                     /translation="MKISTKGRYGLRAMVDLSVYSAGDHISLKSIAERQNISEGYLEQ
                     VFSTLRKAGLINSIKGAQGGYALADKPANITVGAVLRALEGNLSVIDEKSMEEKTNDK
                     IEECIREKVWSAINAGIDRVIDSITLEDLTNEYKRMTSNEAYMYYI"
     gene            complement(1690..3042)
                     /locus_tag="PK733_17495"
     CDS             complement(1690..3042)
                     /locus_tag="PK733_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOJ12361.1"
                     /translation="MKKPLSYALALVLVFSALAGIQSPEAFVVDAVSTATTSGSAPGQ
                     APAAAPDAPAVTGTETGTSRLLADGSFGADVKLLQTMLNSNGYALKVDGFFGKLTQAA
                     VKDYQSKNGLVPDGIVGPKTLAKLVPAPAEPAAPAPASVEPTVTTPAAEENPEVDAVT
                     SASIVSDAAAFEKAISKNGTWIICTLNDLVIDKPLVLEGDFVNTKTPPVSARKIGLYT
                     QDDDHNVTARFTLTAPKLTIKSPDARIQSGTFIGDLYVYSKNFNLKDAKVDGNVYFAT
                     QEAKDTFVMDEKSTITGKQILSDVDAVSSATPTQVQNIEAFEHAISKDGYWIIVPITD
                     LTTDKALVVEGEFHDKNDPTKDLKRKIGLYTHNDPSDKKRATHSFTLTAPSLTIRSPY
                     ATLQYGYFVGDIYVEANNFSLVNNTVTGNIYFATQEAKDTFTMDAGSTVSGVLEVKAK
                     "
     gene            complement(3489..4778)
                     /locus_tag="PK733_17500"
     CDS             complement(3489..4778)
                     /locus_tag="PK733_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOJ12362.1"
                     /translation="MNFRPIAGIDVGKFFSEMAILSPSNEVIARMKIHHDSSSDVERV
                     VELLKKTEKDFDSRPFVVMESTGHYHKILFHSLCKAGLEVSIINPIQTDSIKNIGIRK
                     VKNDKADARKIALLYRFQELKTTNIPDEDIECLRSLCRQYYKLSDELTAYKNRLTGIV
                     DQLMLNFKDVFPNIFSKAALAVLEKYPTPAHILKANRNKLIALIQKNSRRSLKWSTAK
                     YNLLVSKAREFAPLTVHNSSNVAMLGVYISMIRTLEDNLAKVLKTIHLLIAEDMAKDM
                     PMLALTLELLQSLPGIGLLTAATILAEIGDFSAFSKPGKLVAYFGIDPSVMQSGEFTG
                     TRNKMSKRGSRLLRRVLFTTALANIRTKRNKEACNPVLLEFYKQKCQSKPKKVALGAV
                     MRKIIIYIFAVLRDRKPYQLRSPQEHAQMLAAKHIAA"
BASE COUNT         1417 a         1118 c         1011 g         1685 t
ORIGIN      
        1 ttggcaggag tttttttcaa ttgctatatg ttttgaaaca aagccgttga tagatacctt
       61 tctcccgtat ccggaagcag tactacaata tttttgccgg cactgtcagg cctctttgca
      121 gcttccgttg ctgcatgcaa cgctgccccc gaggaaatgc cgaccagtat gccctccgca
      181 gccgcaagct ttctggccgc tgcaaaggct tcctcgcttt ttaccttaac aatctcatct
      241 atgatagaag tgttcaatac attcggaaca aaacctgcgc ctataccctg cagcttgtga
      301 gggcccggtg ttccccccga caataccggt gaatcgtaag gttccactgc aattatcctg
      361 atatcgggat tcttttcttt aagcttctca ccgactcctg tgacagttcc gccggtaccg
      421 actccggcaa caaatatatc tacctgtccc tcggtgtctc tccagatttc ttctgctgtt
      481 gtattcctgt gagctgctga gttcgcggga ttattaaact gctgcggtat aaatgcattc
      541 gggatctcag aaaccaactc ttcagctttt tttatggcac ccttcatccc atcactgccg
      601 ggggtaagaa ccagttctgc tcccaatgct gcaagaaggc ttcttctttc tatgctcatt
      661 gtttcaggca ttgtaagtat aagcctgtaa ccccttgccg ccgcaacaag tgcaagagct
      721 atgcctgtat tgccgctggt gggctctatt attaccgtat ccctgttaac aagccctttt
      781 tcttcggcat cctttatcat tgcatatccc accctgtcct ttacgctgcc tcccggattg
      841 aagtattcca gctttactat aatctttcct tttatgccgt tttggctgtt ataattgacg
      901 gtttccaaaa gcggtgtatt accgataagt tcagtgatac tttttgaaac ttttttcatg
      961 acaatattcc tccattttat actatttgtc ctatttgaat agtaggtttt gcgttaaaat
     1021 attatatgta atacatatat gcttcattgc ttgtcatcct tttatactcg tttgtcaaat
     1081 cctccagtgt aatgctgtct atcaccctgt caatgcctgc gttaattgca ctccatacct
     1141 tttcccttat acattcctca attttgtcat tagttttttc ttccatgctt ttctcgtcaa
     1201 taactgaaag atttccttcc aaagccctca atacagcgcc cacagttata tttgcaggct
     1261 tatccgccaa agcatacccc ccttgtgctc cttttatact attaatcagg cctgcttttc
     1321 tcagggttga aaagacctgt tccagatagc cctctgatat attctgtctt tcggctatgc
     1381 ttttcaatga tatatggtct cctgcagagt ataccgaaag atctaccatt gcccttaacc
     1441 catatctgcc ttttgtagaa atcttcatgt ttatctccta ctattccaat atgttttatt
     1501 atgttcatat tatagcatcg ttttttaaaa attacaatca aaaaatgcaa ctttgttgca
     1561 tttttataat taaagattat ttcattattg gttattataa ttgatacacc ttatatgcaa
     1621 tatagttgtt ctatagagca tataaggtgt ttttattgct tattattgaa tctttacttt
     1681 aaactttttt tattttgcct tcacttccag cactccggat acggtgctgc ctgcgtccat
     1741 tgtgaatgtg tctttggctt cctgtgttgc gaaatatatg tttcccgtaa ctgtgttgtt
     1801 tactaaagag aagttgtttg cttcaacata aatatcacca acgaagtatc catactgaag
     1861 tgttgcatat gggctcctta tagtcaatga tggtgctgtt aaagtaaatg aatgggtcgc
     1921 tctctttttg tctgaaggat cgttatgtgt atagagtcca atcttacgct taagatcttt
     1981 tgtcggatca ttcttatcat ggaattctcc ttcaactaca agagccttat cggttgtaag
     2041 gtctgttatg ggaacaataa tccaatatcc gtctttgctt attgcatgct caaaagcttc
     2101 tatattttgt acctgtgtag gtgttgctga tgatactgca tctacatcag acagtatctg
     2161 ctttccggtt attgtgcttt tttcatccat aacgaatgta tctttggctt cctgtgttgc
     2221 gaaatatacg ttaccgtcaa ccttagcgtc ctttaaattg aaattctttg agtagacata
     2281 gaggtctcct ataaatgtac cgctctgaat tcttgcatcg gggctcttta tagttaactt
     2341 tggagctgtc aaagtaaatc ttgctgttac attatggtca tcatcctgag tataaagtcc
     2401 aattttacgt gcagataccg gaggagtctt tgtatttacg aaatctcctt ctaggacaag
     2461 gggcttatcg ataactaaat cattcaatgt gcaaatgatc catgtaccat ttttgcttat
     2521 agccttttca aaagcagccg catcagacac tatagaagct gacgttactg cgtctacttc
     2581 cggattttct tcagcagcag gcgtagttac ggtcggttcc acagaagccg gtgcgggtgc
     2641 agccggttcc gccggagccg ggacaagttt cgcaagagtc ttaggcccaa ctattccatc
     2701 agggacaagg ccatttttac tctgatagtc cttaactgca gcttgagtca gcttcccaaa
     2761 aaagccatcc actttaagtg catatccatt gctgttaagc attgtttgca aaagcttaac
     2821 gtctgctccg aatgaaccgt ctgcaagtaa acggcttgtg cccgtttccg taccggtgac
     2881 agcaggtgcg tcaggtgcag ctgccggagc ttgtccgggt gcagaaccgc ttgttgttgc
     2941 tgttgaaaca gcatctacaa cgaaggcttc cgggctttga attcctgcaa gggctgagaa
     3001 tacaagtacc aaagctagtg cataagatag tggttttttc aaaaagatac ctccttaaaa
     3061 ttatttgatt taatattgct gcaaagtaaa ccacctttaa tagtataaag attaacttgt
     3121 ttgttaattt tcaaactatg aaaacgatta ttattctgta gcaatattga tagtatttat
     3181 tataacaaaa cctttttcaa aagtacatgc catatttggc atatttcttt agttttagag
     3241 aaaatttcat taaagccata ggtgcattcc gctatcataa taacaatatt tattcatata
     3301 ttgttaatga atgaaagacc agctaattaa agtcaagagt ttttaagaaa aagctttatg
     3361 aatttttgaa aactcctgac atagaaaaaa tgcataacaa caagaccacc tttacgaagt
     3421 agacataaaa tagctggtct aaaatgaaat actgatcctt gaaaactaaa catcaagtgc
     3481 aagtactgct aagctgctat atgctttgct gctaacatct gagcatgttc ctgaggactg
     3541 cgtaactggt acggttttct gtcccttaga acagcaaaga tgtaaatgat gatcttacgc
     3601 ataactgctc ccaaagctac cttcttaggt ttgctctggc acttttgctt gtagaattca
     3661 agtaatacag ggttgcaagc ctctttattc cgcttagttc ggatattggc aagagcagtt
     3721 gtaaaaagaa ccctgcgaag tagcctggaa cctctcttgg acattttatt ccgtgtgccg
     3781 gtaaattctc cggattgcat cacagagggg tcaatgccaa agtaagcaac cagttttccc
     3841 ggcttagaga aagcggaaaa atcgccgatt tctgccagaa tggtggcagc agtaagaagg
     3901 ccaatacctg gcaggctctg caaaagttca agagttaatg ctagcatggg catgtccttt
     3961 gccatatctt cagcgatcaa tagatgaatg gtttttagga ccttcgccaa gttatcttcc
     4021 agagttctga tcatggatat gtagacaccc agcatagcaa cgttcgagga attatgaaca
     4081 gtcaaaggcg caaactctct ggctttggag accaaaagat tatactttgc agttgaccac
     4141 ttaaggcttc tgcgagaatt cttctggatc agtgcaatca acttgttcct gttcgcttta
     4201 agaatatgcg caggcgtagg atatttctcc aatactgcaa gagcagcctt tgaaaagatg
     4261 ttggggaata catccttgaa gtttaacatg agttggtcaa caatacccgt aagcctgttt
     4321 ttgtaggcag taagttcgtc agagagcttg tagtactgtc ggcaaagact tcgcaggcat
     4381 tcaatatcct catcggggat attagtagtt ttaagctcct gaaatctgta tagcagggca
     4441 attttccggg catcagcttt atcatttttc acttttctga ttccaatatt tttgatagaa
     4501 tcagtctgga tggggtttat gatggagacc tccaatccag ctttacaaag tgaatggaaa
     4561 aggattttgt gatagtgccc ggtggattcc atgacgacga aaggcctaga atcaaagtcc
     4621 ttttccgttt ttttcagtaa ttcaacgact ctttcaacgt cagaactgga atcatggtgg
     4681 atcttcatgc gggcaatcac ttcattggat ggagaaagaa ttgccatctc actgaagaat
     4741 ttacctacat cgattcctgc aataggtctg aaattcatca aaatgcctcc ttaaaaaatt
     4801 aatggactta aaagcctcca ttccttctca tgtaagcaaa caaccttgca tgtgacacga
     4861 ggaaccagct aaaaagctgg cctcaaccag ccaaatcatg tagacttacc ggaatggaaa
     4921 aatactcttt catacgggta atcggcccgc caaggtccgt cccaggaggt gaaacacaca
     4981 ccttccaagt ccggaagata ttatacatga ttgtcaaagt tcaggccaac aaacgctggc
     5041 ataatggcta agatgtgaag ccggatgatg tgcttgatgt ttggaaaaga ataaaatttg
     5101 aagtgttatg ttaactatga cttttaatgg gtcgaacagt ggctttggtc actgatttaa
     5161 tacaaaataa ttgtacaagg aggtaataca gatgaaaaga atagtagcaa ttgttactat
     5221 agcagcaatt a
//
LOCUS       DAOSDD010000283         5200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5665_length_5200_cov_3.465695, whole genome shotgun sequence.
ACCESSION   DAOSDD010000283 DAOSDD010000000
VERSION     DAOSDD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5665_length_5200_cov_3.465695"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..241)
                     /locus_tag="PK733_17505"
     CDS             complement(8..241)
                     /locus_tag="PK733_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12363.1"
                     /translation="MLCISLGISYIETIFINGKEYINTDYMPELKINLGVPYIQSVKI
                     KGREYTNISFRDGSIGKNIKTNMIDKDEKYKNK"
     gene            complement(294..1994)
                     /locus_tag="PK733_17510"
     CDS             complement(294..1994)
                     /locus_tag="PK733_17510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ12364.1"
                     /translation="MLIWKTGADGVRTFINNSFFRLAGEDIKEANSGWFSIVHPDDLS
                     KCLNKYYEALKFGKSFRTRYRILNNSGEYRWMLEIGMPLSHISKDLTGYIGVCIDITM
                     RKTLKKALKEKLQVYKSAFEINNMPTLVLSLEEFGIVDANPAAEKFYGYSRNKLKKME
                     MMDLITGIDINREIKKTIEEKQRLLSLAENFKDGEIKYAKAHCSLMLTVVGNLVFIMI
                     NEDIDNKEILAQLAYKDYHDELTGLYNRKYFELQLKHLDNEIQLPLSIIIGDVNNMKL
                     INNSFGYDKGDEVLKAIAQILRLSCRKEDIICRIGGDEFALILPGTREKDAAKICNRI
                     REKCKSQDQKLLKYEISLGTAARTKKTQDIVDICREAEDKMYKNKLSEGTSLRSSLID
                     SLRKTIEERTWETEAHAERMKDRALKLGRLLNLSNAELDELALLATLHDIGKIGIPDS
                     ILSKKGVLNTEEREIMKKHSEIGYRIAAASPELSNIANAILYHHEHWDGNGYPHGLKG
                     KEIPITSRIISVIDAYDAMTNDRVYHAAISKEKAVEEITNCSGKQFDPRVVEAFLKMM
                     "
     gene            complement(2208..2903)
                     /locus_tag="PK733_17515"
     CDS             complement(2208..2903)
                     /locus_tag="PK733_17515"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12365.1"
                     /translation="MIDIHSHILFDIDDGARDMDESVRMIHAAGKEGIKVIIATPHFR
                     EHMLNPEKTYDNYRILLEKTRNIGIDLKLGFEIALNPILAKAVNIVDKHSLNATKYVL
                     VEFPYFGFSEHSFKLLDLFQKNKFKQIIAHPERNGRGFGSKRTISKLKELGYLIQVNA
                     GSIAGTYGVRAKGMAKYLIKNKIADFVASDAHRESYYSWYSKAWQNVIKWSDEDYANK
                     LFYENGKKILNTM"
     gene            complement(2923..3690)
                     /locus_tag="PK733_17520"
     CDS             complement(2923..3690)
                     /locus_tag="PK733_17520"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ12366.1"
                     /translation="MVLTKYEKLNELSIPAEEAYKTLRTNIRFCGVVNKIKTITITSC
                     APGEGKTTTSINLAISIANAGMKTLLVDTDFRRPMIEKSLGINTKAGITDYIMGETDL
                     ESIIYKTSIENFYITPCGFVPPNPAELLSNEKFSEFIKNVRDQYLKVVRGQLDMIIFD
                     TPPLGSVIDAAILSAQTDGVLLVIKSKSTNYKIAQEVKGQLEKANAYILGVVLNKIPK
                     KDLKYGYRNYYNYYNKDSTDQNTSPFKRLMRKTKLIS"
     gene            complement(3704..4372)
                     /locus_tag="PK733_17525"
     CDS             complement(3704..4372)
                     /locus_tag="PK733_17525"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOJ12367.1"
                     /translation="MELKEYLNIIIKRIWLIILLPLIASMVSAYVSFYIIDQVYEANT
                     TLYIINKSNASELSIAYNDLLVGQKLVEDYKEIVKSRRITSEVVEELDLKNITSSMLA
                     EKINVSAKNETRLIEIRVQNKVPQLAADIANKVADVFTREIVKIMRVENISIVDTAQL
                     PEHPIKPRPMMNIAVAFMVGLLTALGIVFIIEYLDDTIKTTDDVERYLGVTVLGTIPE
                     FTIK"
     gene            4822..5178
                     /locus_tag="PK733_17530"
     CDS             4822..5178
                     /locus_tag="PK733_17530"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ12368.1"
                     /translation="MIGSRIRQLRKEHNLSQNQFASLFGVYDSTISQYENGKREPEYD
                     TVIKIANQFNVSIDWLLGRMNSKDLVKLENDNLPEQLRDVGVEYLILAKEMKEKEIPP
                     EDVRKIIDAINALKKK"
BASE COUNT         1556 a         1008 c          677 g         1959 t
ORIGIN      
        1 ctatttccta tttgttttta tatttttcat ctttatctat catatttgtt tttatatttt
       61 ttccaatact cccatctcta aaactaatat tagtatattc cctacctttt attttaacag
      121 attgtatata tgggacacca aggtttatct ttagttcagg catatagtct gtatttatgt
      181 attctttgcc gtttataaaa atagtctcta tgtatgagat cccaaggctt atgcataaca
      241 ctactatcgt ctcctttttc actttttgct agtaaattta tattacattc tccctacatc
      301 attttcagaa atgcttctac aactctagga tcaaactgct taccactaca gttagttatt
      361 tcttcaacag ccttttcttt tgaaatggct gcatgatata cccgatcgtt ggtcattgcg
      421 tcatatgcat ctattacact aataattctt gatgttatag gtatttcttt acctttaagt
      481 ccatgaggat aaccatttcc atcccaatgt tcatgatgat atagaattgc atttgcaatg
      541 tttgataact ccggactggc agctgctata cgatatccta tttcactatg ttttttcata
      601 atctcccgtt cttcagtgtt aagaacccct tttttggata atatgctgtc aggaatacct
      661 attttcccaa tatcgtgaag agtagccaat agagcaagtt catctagttc agcattagaa
      721 aggtttaata atctcccaag ttttaatgcc ctgtctttca ttcgctcagc gtgagcttca
      781 gtttcccagg ttctctcttc aatggttttt cttaatgaat ctattaatga actccttaaa
      841 cttgtcccct cacttagttt gttcttgtac atcttatctt cagcttccct gcaaatatcg
      901 acaatatcct gagttttttt tgttcttgcg gcagttccta atgatatctc gtatttgaga
      961 agtttttgat cctgagattt gcatttttct cttatacggt tacaaatttt cgcagcatct
     1021 ttttccctgg ttcccggtaa tattaaagca aactcatcac ctcctattct gcaaattata
     1081 tcttcctttc tacaggaaag tcttagtatt tgggcaattg cctttaagac ttcatctcct
     1141 ttatcatagc caaaactatt gtttattaat ttcatgttgt ttacatcccc gataataata
     1201 cttaaaggta gctgaatttc attatccaga tgtttgagtt gcaattcaaa gtactttcgg
     1261 ttataaaggc cggtaagttc atcatggtaa tccttatatg ctaactgtgc caggatttct
     1321 ttattatcaa tatcttcatt aatcattata aataccaaat ttcccaccac tgtcaacatc
     1381 aagctgcaat gagccttagc atattttatt tcaccgtctt taaagttttc cgcaagagag
     1441 agaagacgct gtttttcttc tattgttttc tttatttccc tgtttatatc tattccggta
     1501 attaaatcca tcatttccat ttttttaagc ttatttctgc tgtagccgta aaacttttct
     1561 gctgcaggat ttgcatcaac aataccaaat tcttcaagac tcagaactag tgtcggcata
     1621 ttatttatct caaaagcact tttgtagact tgcagttttt cttttaaagc tttttttaac
     1681 gtttttctca tggtaatatc aatacataca cctatatagc ctgtaagatc cttactaatg
     1741 tgactcaggg gcatacctat ttccagcatc cacctatatt caccactgtt attcagtatc
     1801 ctgtatcttg tcctaaaaga ctttccaaac tttaaagctt cataatactt gttaagacat
     1861 ttactcagat catcaggatg aactattgaa aaccacccgc tattagcttc ttttatatcc
     1921 tctcctgcta acctgaaaaa actgttatta ataaaggttc gtactccatc agcccctgtt
     1981 ttccatataa gcacacgaat ttcccccatt taaaattact atatcaattt cagtcttttg
     2041 aactctccca taactaaagt cacgaatgtt cccgataata ctataaaata tgtcttctat
     2101 atgcaattcg acataatatt ataaaatcct tcaaaatttg taacttttca taaatattat
     2161 acagaaaaat tggaataaaa agtttttaca ttgatactaa tattgaatta catagtgttt
     2221 aggatctttt ttccattttc ataaaaaagc ttattggcat aatcctcatc agaccatttt
     2281 attacattct gccatgcctt gctataccag gagtaataac tttccctatg ggcatcagat
     2341 gctacaaaat ccgcaatttt gttttttatt aaatattttg ccataccttt tgctcttacc
     2401 ccataagttc ctgctatact accggcgttt acctgtataa ggtaccccaa ctcttttagt
     2461 ttgcttattg tccttttact tccaaatcct ctcccatttc tttcgggatg cgcaattatt
     2521 tgcttaaact tgtttttctg aaataagtcc agcaacttaa aactatgttc actaaaacca
     2581 aaataaggaa actctaccaa tacatattta gttgcattca aactgtgttt atcaactata
     2641 tttacagcct ttgccaatat tggattcaac gctatctcaa accctaattt caaatctatt
     2701 cctatattcc tggttttctc caataatatc ctatagttat cataagtttt ctcaggattt
     2761 aacatatgtt cacgaaaatg gggagtagct attataacct ttattccttc cttccctgct
     2821 gcatgtatca ttcgcacaga ttcatccata tctcttgcac catcgtctat atcaaaaaga
     2881 atatgactat gtatatcaat cactctttta ccttccaatc tactaagaaa taagtttagt
     2941 tttcctcatt aacctcttaa agggacttgt attctgatca gttgaatcct tgttataata
     3001 attgtagtaa ttcctatagc catattttaa atcctttttt gggattttat ttaagacaac
     3061 gcctaaaatg tatgcattgg ctttttctaa ttgccctttt acctcctggg caattttgta
     3121 attagttgat ttagacttta taaccagtaa aacaccatca gtttgggctg ataatatagc
     3181 tgcatctata acactaccca atggaggagt atcaaatatt atcatatcca actgacctct
     3241 tactaccttt aaatactgat ctcttacatt ttttataaat tccgaaaact tttcattgct
     3301 taataattct gcaggattag gaggaacaaa cccacatggg gttatataaa aattttctat
     3361 gcttgtcttg taaataatgc tttccaaatc tgtttctccc attatataat ctgtaatacc
     3421 agctttagta ttaataccaa gggacttttc tatcattggc cgtctaaaat cagtatctac
     3481 aagcaaggtt ttcattcctg cattagcaat gcttattgcc agatttattg aagtagtggt
     3541 tttaccttca cctggcgcac aacttgttat ggttattgtt tttattttat taactacacc
     3601 gcaaaagcga atatttgttc taagagtttt atatgcttct tcagcaggta tactaagttc
     3661 atttagcttt tcgtactttg tcaataccat ttaataattc ctcctatttt attgtaaact
     3721 ctggaatggt tccaagtaca gttacaccaa gatacctttc tacatcatct gttgttttta
     3781 ttgtatcatc taaatactca ataataaata caatccctaa agcagtaaga agtcctacca
     3841 taaaggctac cgctatattc atcattggtc tgggttttat agggtgttcc ggcaactggg
     3901 cggtatctac tatactaata ttctcaaccc tcataatttt tacaatttcc ctggtaaaca
     3961 catcagctac cttattggcg atatccgcag ccagttgggg tactttattc tgtactctaa
     4021 tctcaatcaa tctggtttca tttttggcac ttacatttat tttttcagcc aacattgatg
     4081 aggtaatatt tttcaaatcc aattcctcaa caacttcact ggtgattcgc ctgcttttta
     4141 ctatttcctt atagtcttct acaagcttct gccctacaag aagatcatta tatgctatag
     4201 acaactccga agcattagat ttgtttatta tatatagtgt agtattagct tcgtaaacct
     4261 gatcgattat ataaaagctg acataggctg aaaccattga agcaatcaga ggcagcaata
     4321 taatgagcca tatcctctta attattatgt ttaaatactc ttttaattcc attaatcttt
     4381 cacctgattc caattaaatt ttaaagtgat aacgcgttaa tattcataag gccttgaaac
     4441 acctctttag taatcatccc cttatcactt taaatcatta ttttaatacc ttttaaacca
     4501 tataaattta aatcctaaaa ccaattattt actactgcat caaacatatt ttactgttta
     4561 tctcttattt tgtcaacatt tatacttttc gagtttttgt acatttatat aagcacatct
     4621 tctataacca cctttgttta tctgccatat aatatttcgt caatttactg atttatatta
     4681 attttatttt aaaatgtatt aaatcaatat ctaattataa tacctgataa taatatttac
     4741 ttattataat ttaaatagta taatatgtct cataggaagc cgggattaat tgggattaat
     4801 tcagaagggg agtataatgt aatgataggt agcagaataa ggcagcttag aaaagagcat
     4861 aatctctctc aaaatcaatt tgcatctttg tttggtgtat atgacagtac catatcacag
     4921 tatgaaaatg gtaagaggga accagaatac gatactgtta taaaaattgc caatcaattt
     4981 aatgttagca tagactggct tctcggaaga atgaacagta aggatctggt aaaattagaa
     5041 aatgataatc tgcctgaaca attaagggat gttggtgtgg aatatttaat attagctaag
     5101 gaaatgaagg aaaaggaaat acccccagag gatgtgcgaa agataattga tgcaataaat
     5161 gctcttaaga aaaaatagta ttgactaaaa taaatatatt
//
LOCUS       DAOSDD010000284         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5689_length_5180_cov_4.978732, whole genome shotgun sequence.
ACCESSION   DAOSDD010000284 DAOSDD010000000
VERSION     DAOSDD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5689_length_5180_cov_4.978732"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="PK733_17535"
     CDS             <1..2078
                     /locus_tag="PK733_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12369.1"
                     /translation="DLAIATGDVMNAMLKINYALNYLLQSDEIKAFGMEMVRSLQKIS
                     DGITQAIYAMERINSAVKKLSEQIDLDKLSEGVEYLNQSIDDLSSAIACIQDIKPYLE
                     AARPYFEIASNYASQAFASAIEATDSLERSGAAMADAAAQIRDLISDLAKQPEITVKK
                     VDSKYMETKNRFSEALGETLNALSDLNSIISGASPTLLADIKAISNQLFVVFDLLVDT
                     VENVSEMNTDIRDYTKDISTRDTDSDTSGKVADSVNNGHVQGDINVGGVAGSMAIEFG
                     YDLEDEYNPIQKMSPNSKYLLRAIISGCENYGNIISKKNCAGGIVGLMDFGYVKESID
                     NSSISSTGGNYIGGIAGKSDGTIRQCYAKSFLYGADFVGGIAGYGTNLYNCYSMIQVE
                     SAREFVGAIAGNGEGLWEGNYFVHDELAAVNRISYAGKAEPITYEVLSSVEGLPAIFK
                     TFRLTFVAEDEEIAIVPFNYGDTVPENQIPYVPKKDGYFGKWEKDDYRNLKFDATIKA
                     TYEQYITTLASKQTRDNGLSVILVNGLFDKDASLIVTEGEKDLEGEKNPEREKNSGGE
                     GLFTDKQALEEWTVSVTDDGQTTRMVHYLAPVQKTSGISIYLLQDGIWKKAESTAKGK
                     YLIFEIEGTDATFIVTYSEKNAVKTVLIIMFISSVIAAFLWLRRRRGIRINESGDISA
                     KYDSIVITK"
     gene            2092..3345
                     /locus_tag="PK733_17540"
     CDS             2092..3345
                     /locus_tag="PK733_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOJ12370.1"
                     /translation="MINLFKQCLINYGISENMSLYISNAIAILVIILLSIIADIIAKK
                     LLLKALKLYISKSKNKWDDILIQKKVFERLFHIVPIIVIHAFAPVFPDYKDLIQKFAF
                     SYIILAVIFAIDRLLDAIDDIYRNYEVSKIRPIKGYLQLIKILFYVIGIVIVVSTFLE
                     RSPWILISGIGAATAVLLLIFQNSILGFVASIQLTTNNMLQIGDWIEMPKYGADGDVI
                     DMSLHTVKVQNWDKTITTIPTHALISESFKNWRGMQEAGGRRIKRSIYIDMTSIKFCT
                     EEMLERYKQIEYITDYLIKKKLEIENYNKTHAKDSTSIVNGRHLTNIGTFRAYVEQYL
                     NNHPNVHKGMIHVVRHLQPTENGLPIEIYVFTNETAWVDYEKIQADIFDHILAVIPEF
                     DLRIYQSPTGHDLRKTINNNFTDYT"
     gene            complement(3304..3756)
                     /locus_tag="PK733_17545"
     CDS             complement(3304..3756)
                     /locus_tag="PK733_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12371.1"
                     /translation="MQRIVLGRKNSIFNRLIIVFLLVMIPIYVLAVNIYYWGINTIKN
                     EILDSLKAQNTFYLNSLESEIERIKKMQYDCINDDDLNNLAYASQLMDNIEMTYAIKR
                     LQKRLTIINDSSICIKGVCACCISCQARNTTANTTSCIVGKIVINGFS"
     gene            complement(3776..4897)
                     /locus_tag="PK733_17550"
     CDS             complement(3776..4897)
                     /locus_tag="PK733_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ12372.1"
                     /translation="MNNDVKLFGSLYGLNDAWVSWLKGEKFPIAFYGDSTVDGNNTTG
                     WIGNKLGADSTSPNAFSEKLEIKLREAANNSILRIYNAGFSGQISSWALANYDNVFGE
                     TSAYNDVKMVGIGFGINDRLVYQDEKSYRDGFKNNIKGIIGKCYASNIQPFLITTQAT
                     VEPGVRTDFTTRYPLRTSQHINTIANEVKKELSIEYGLELLDINSYTEDFLLYSSYSL
                     ETIISDRLHFNDVGHEYEAGLLFALINPQTIFADGFHKIDYSNQRIAKGVPEDWLTMP
                     TAVKDNFKVYIDYTKSDMADMNIMTVYVFNKSKRKLNLKAYKNHETTKTYVKIDGEVK
                     SLTGVETDLGELELGLHKFEVFTGTSNIADFKGFLLVML"
BASE COUNT         1688 a          894 c          956 g         1642 t
ORIGIN      
        1 ctgatttagc tattgccacc ggggatgtca tgaatgctat gctcaaaatc aattatgctc
       61 ttaattattt gcttcaatcc gatgagataa aggcttttgg gatggagatg gtccgaagtc
      121 ttcaaaaaat ttccgacgga attactcaag caatatacgc aatggaaaga ataaactctg
      181 ctgtgaaaaa gttatcagag cagattgacc ttgacaagtt gagcgagggc gttgaatatc
      241 ttaatcaatc tattgacgat ctaagcagtg ctattgcttg tatacaggat ataaagcctt
      301 acttagaagc tgcaagacct tattttgaaa tagcatcaaa ttatgcttca caggcttttg
      361 cttccgctat agaagcaacg gattcgcttg aaaggtccgg ggctgccatg gcagatgccg
      421 ctgcacaaat tcgtgattta atctcagacc ttgcaaaaca gccggaaata accgtaaaaa
      481 aagtagacag taagtatatg gaaactaaga atcggttttc tgaggctctc ggggaaactt
      541 taaatgcttt atccgacctg aattcgatta tttcaggggc atcccctaca ttgcttgcgg
      601 atatcaaggc aatttcaaat cagctgtttg ttgtgtttga tctgttggta gatactgttg
      661 agaatgtatc agaaatgaat acagatatca gggattatac aaaggatata tcaactcggg
      721 atacagattc ggatacttct gggaaagtag ctgacagcgt aaataatggg catgtacaag
      781 gagatattaa tgtaggcggt gttgccggtt caatggccat cgaattcgga tatgatttag
      841 aagatgaata taatccgatt caaaaaatgt ctccaaactc taaatatcta ttacgagcca
      901 ttatatccgg ttgtgagaac tacggcaata ttatatcgaa gaagaactgt gccggcggaa
      961 ttgtaggctt aatggatttc ggatacgtaa aagaatctat agacaatagc agtatcagca
     1021 gcaccggcgg taattatatt ggaggaattg caggaaaatc tgatggcaca atacggcaat
     1081 gttatgcaaa aagtttttta tacggagccg attttgtagg tggtattgcc ggatatggca
     1141 ccaatctata taactgttat tcgatgattc aggtggaaag tgcccgggaa tttgttgggg
     1201 caattgcagg aaacggtgag ggtctttggg agggcaatta ttttgttcat gatgaattag
     1261 ctgctgtaaa tagaataagt tatgccggaa aagctgagcc gattacctac gaggttcttt
     1321 catcggttga ggggttgcct gcaattttta aaacttttcg gctgaccttt gttgcagagg
     1381 atgaagaaat cgctattgta ccttttaact atggggatac tgttccggaa aatcagatcc
     1441 cctatgttcc taagaaagac ggatattttg gcaaatggga aaaagatgat tatagaaacc
     1501 ttaaatttga cgcaactatt aaggcaactt acgaacaata cattacaaca ttggcaagca
     1561 agcaaacccg ggataatggt ttgtctgtca tccttgtaaa tggcttgttt gataaggatg
     1621 cctctttaat agttactgaa ggagaaaagg atcttgaagg agaaaagaat cctgaaagag
     1681 aaaagaactc cggaggagaa ggtttgttta cagataaaca ggcattagaa gagtggactg
     1741 tttctgtaac ggacgatggg cagacaactc gcatggttca ctatcttgcc cctgtccaaa
     1801 aaacctcagg catcagtatc tatctcttgc aggatggcat atggaaaaaa gcagaatcca
     1861 ctgcaaaagg aaaatatctt atttttgaga tagagggaac agatgcaaca ttcattgtaa
     1921 cttattcaga aaagaatgct gtaaagactg ttctgattat tatgtttata tcttcggtga
     1981 tagccgcttt cctatggctg cgtagaaggc gaggcattag aattaatgaa tccggcgata
     2041 tctctgctaa gtatgatagt atcgtaataa caaaatagga ggactttggt aatgattaac
     2101 ttatttaagc aatgtttgat aaattatggt atcagcgaaa atatgtctct ctatatatct
     2161 aatgctatag caatattagt tatcattctt ttgagtatta tagccgacat aatagcaaaa
     2221 aaacttttac ttaaagcttt gaaactttat atctcaaaaa gcaaaaataa atgggatgac
     2281 atacttatac agaaaaaagt atttgaacga ttgtttcata ttgtaccaat cattgtgata
     2341 catgcatttg ctcctgtatt ccccgattat aaagacttga tacagaaatt tgccttttct
     2401 tatatcattc ttgctgttat atttgcaatt gataggttgt tggatgctat tgacgatatt
     2461 tacaggaatt atgaagtatc taaaataagg ccaattaaag ggtatctcca gcttataaaa
     2521 attttgtttt atgtaattgg catagttata gtcgtcagta cctttctgga acgttcgcca
     2581 tggattctca taagtggcat aggtgctgca acagcagttc ttctactaat ctttcaaaat
     2641 tcaatacttg gatttgtcgc aagcatacag cttaccacaa ataatatgct gcaaatcggt
     2701 gattggatag aaatgccgaa atatggagct gatggagatg ttatagacat gtcgttgcac
     2761 accgtgaagg tacagaattg ggataagact attactacta ttccaactca tgcactaatt
     2821 tcagaatcat ttaaaaactg gcgtggaatg caggaagcag gcggaagaag aataaaacga
     2881 tcaatatata tagacatgac cagtataaaa ttctgtactg aagaaatgct ggaaagatat
     2941 aaacaaattg agtatattac agattattta ataaaaaaga aacttgaaat tgaaaactat
     3001 aataagacac atgccaaaga tagcacaagt attgttaatg gcaggcattt aaccaatata
     3061 ggtactttca gagcttatgt agaacagtat ttaaataatc atcccaatgt acacaaaggt
     3121 atgattcacg tagtcaggca tcttcagcct actgaaaatg gtttacccat agaaatatat
     3181 gtttttacaa acgaaacagc atgggtagat tatgaaaaaa ttcaggccga tatttttgat
     3241 catatcctgg cagtaatccc ggaattcgac ctgcgtattt accagagtcc taccgggcat
     3301 gacttaagaa aaaccattaa taacaatttt accgactata catgaagttg tatttgctgt
     3361 tgtatttcgt gcctggcacg aaatgcaaca cgcacataca cctttaatgc atatactgct
     3421 gtcatttata attgtaagac gcttttgcag tctttttatt gcataggtca tttctatatt
     3481 atccattaac tgagatgcat atgcaagatt gttcaaatca tcatcgttta tacaatcgta
     3541 ttgcattttc tttattctct cgatttcact ttcaagacta ttcaaataga atgtattttg
     3601 agcctttaat gagtctaaaa tctcattttt tattgtattg ataccccagt agtaaatgtt
     3661 tacagccaat acatatatag ggatcataac caataaaaac actattataa gtctattgaa
     3721 aattgagttt ttccgaccca aaacaattct ttgcatataa ttataccccc aaaactcaaa
     3781 gcattactaa taaaaaacct ttgaaatcag caatattgga tgttcctgta aacacctcaa
     3841 atttatgtaa tcctaactcc aattcaccta aatctgtttc cactcctgtt aagctcttta
     3901 cttcaccatc aatttttaca taggtttttg ttgtctcgtg atttttatat gcctttagat
     3961 ttaatttcct ttttgattta ttaaacacat atactgtcat aatattcatg tctgccatat
     4021 cagatttggt ataatcaata taaactttaa aattatcttt tacagcagta ggcattgtta
     4081 accaatcctc aggaacacct ttagcgatac gttgatttga gtagtctatc ttatggaaac
     4141 catctgcaaa tattgtctgt gggttgatta aagcaaataa gagccctgct tcatactcat
     4201 gacctacatc attaaaatgg agtctatctg atataattgt ttccaggcta tatgatgagt
     4261 aaagcaaaaa gtcctctgta tatgagttta tatctagtaa ttcaagtccg tactcaatag
     4321 acaattcttt tttcacttca tttgcaatag tattgatatg ctggcttgtc ctcaatggat
     4381 atcgagtagt gaaatcagta cgcacccctg gttcaacagt agcttgtgta gttattaaaa
     4441 aaggttgaat attacttgca taacatttac caataattcc tttaatattg tttttgaaac
     4501 catctcgata agacttttca tcctggtaaa ctaatctatc gttaatgcca aaccctattc
     4561 caaccatttt aacatcatta tatgcagaag tttcaccaaa tacattgtca taattggcta
     4621 aagcccagct tgatatttga ccgctgaatc cggcattata tattcttagt attgaattat
     4681 tggcagcttc tcttaattta atttctaatt tttcagaaaa agcattaggg cttgtactat
     4741 cggcacctaa cttattacct atccatcctg ttgtgttgtt tccgtcaact gtactgtcgc
     4801 cgtaaaaagc aattggaaac ttttctcctt taagccagga aacccatgca tcatttaatc
     4861 catatagtga gccgaataat tttacatcat tgttcatctg taatcactcc acttattata
     4921 aaataaattt ctcttattgt atcatattgc agtgtatggg tgcaataaat tggtgtgttt
     4981 acatgtttac ggtgtttttt tacctacgcc gttgtaaaac gtgccgggct aaaaacacaa
     5041 cggcaattgg tgctgttgca attggtgctg ggctaaaaac acaacacaaa ctgcacatta
     5101 agggatataa caacccctcc tgaatatccc taattctgca gtaaaaattt aacaaatcat
     5161 taatcaaacc cttgatacaa
//
LOCUS       DAOSDD010000285         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5818_length_5099_cov_5.839413, whole genome shotgun sequence.
ACCESSION   DAOSDD010000285 DAOSDD010000000
VERSION     DAOSDD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5818_length_5099_cov_5.839413"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PK733_17555"
     CDS             complement(<1..856)
                     /locus_tag="PK733_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12373.1"
                     /translation="MDFKELIAINGDQSKNEMILGQLLYMSLGSMLIKREDMQDILEI
                     LGIDEKYMPRKLQYTDAFRSATTEASTKLSVNGKCIKLYMRDNLKDKSKGIISRELVK
                     EIVGWDNHYTQVGKFRYDTENKTMSYELADYNSDISVHQLQELADKVEALFELYKESY
                     NRNSVETIINNLLENMDTSKVTQHGKLYFVPRYKLNEVMKLEELGELLNEKNYLANMG
                     VNYAPVTINAIYVINDDKQRKNFEQSFYTTVQNELELYQNRLTHFINNGSKSEKIIGR
                     WLEKIKELN"
     gene            1282..1542
                     /locus_tag="PK733_17560"
     CDS             1282..1542
                     /locus_tag="PK733_17560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04540 family protein"
                     /protein_id="HOJ12374.1"
                     /translation="MYTFLHYPITVWELATEIKRVFNDYYLRRIDEKQLHACILTWVD
                     NCPNKLFKDDEINPTVKAVIGKNREFVLKKIINNLCDSSIAQ"
     gene            1595..2989
                     /locus_tag="PK733_17565"
     CDS             1595..2989
                     /locus_tag="PK733_17565"
                     /inference="COORDINATES: protein motif:HMM:NF040303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HOJ12375.1"
                     /translation="MNQEALELKDMLRIEGINSKGFGIIPKVVMFDPDLSIEAKTIYA
                     YFCSLAGNGTTAFPNRDTILFYLNITKDYYYKHFNQLKEQEFIKVERNSNFPYNNIYT
                     LINNPKKYEEKMSNFDKNKPYSQLKYSGLKAHGFGIVPRIIMMDRRLHIKAKGIYAYF
                     CSLIGNGITAFPQINEILFYLHIGRQAYYKYYNQLVSCNYITVVQRKTDSGTFSVNDY
                     YLNDNPDYNIGQQRQGEKQENLPCDDLQDNDKCIDNTGFQPCDDLPDNDLPCDDLPDN
                     DLPYDDLPYDDLPDNNNINSININSININKSINQSNDKQKALRKSTKKKMIDKIDKNK
                     KTNKKELPTYQNDYELYKKLIAQNISYEDLKLKFFNENMIGEILNLMTEIVTSDKKYI
                     RVNGEDKPAEIVKSTFLKLNATHIEYVLESIENNTTEINNIKAYMISTLYNAPMTINT
                     YYRSRVNYDLYGSK"
     gene            3180..4706
                     /locus_tag="PK733_17570"
     CDS             3180..4706
                     /locus_tag="PK733_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ12376.1"
                     /translation="MYIPGFRSKKTTKITIAIIYYVFSLLMLFVGISYSLFLISLPFI
                     VFYGIDLLKYKQKKLLVPFIASVLVFVVSIIIFPTNTPKIDADSNTSNTSGITQADKS
                     ISDNEPTNTTVASEDEPDIAKENNKNVVVDEGTANEESIANKGDTGTTLKENITDKDK
                     DKTNPTATVQDESKKEVSGNIKVHFLDVGQADCILIQTSNNKNMLIDAGNNDDSSFIL
                     SYLNNLGIEKIDIAVGTHPHEDHIGSLDDVIKTFGIGKVYMPKVEHSSQTYLDVLTAI
                     KGKELKVTTPVAGEYIDFDIDTKVQILAPNSDKYDNLNDYSIVVRLEYGNTSFLFTGD
                     AETVSEQEIVSKKYNINSDVLKVGHHGSTTSSSLAFIKAVSPKYSVISVGKDNKYGHP
                     DSIIINRFKMFGEVLRTDESGTIIITSDGEKITIEKKASSIKENAPPEESQEVNKPAP
                     STQEEPSKQEGIYIGNINSKIFHRETCTTLPDQKNQVKFNNREEAIDAGYTGCKRCNP
                     "
     gene            4727..4939
                     /locus_tag="PK733_17575"
     CDS             4727..4939
                     /locus_tag="PK733_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HOJ12377.1"
                     /translation="MKLIIDRFEGEYAVCENEKRKIINIEKSKLPTAAKEGDTIIKEN
                     GEYIIDVDDSKNRKETITNLMDELFD"
BASE COUNT         1829 a          742 c          862 g         1666 t
ORIGIN      
        1 cattaagttc tttgattttt tccagccatc tgcctataat cttttccgat ttgctgccat
       61 tgttaataaa gtgcgttagc ctgttttgat acaattccaa ttcattttgt accgttgtgt
      121 agaaactttg ctcaaagttc tttctttgct tatcgtcatt tatgacatat atggcattaa
      181 tagttacagg agcgtaattt actcccatat ttgctagata gtttttttca ttcagtagtt
      241 caccaagttc ttctagcttc attacttcat ttagtttata acggggaaca aaatacagtt
      301 tcccgtgttg tgtaactttt gaagtatcca tgttttcaag cagattattt attatggttt
      361 ctactgaatt tcggttatag ctttctttgt atagctcaaa taaagcctca accttatcag
      421 ccagctcctg cagctgatgt acacttatat cgctgttata gtctgcaagt tcatatgaca
      481 ttgttttgtt ttcagtatca tatctaaact tgcctacttg cgtatagtgg ttgtcccatc
      541 ccactatttc ttttaccagc tctctactga ttatgccttt acttttgtct tttaagttat
      601 cacgcatgta cagcttaatg cactttccat taacactaag cttagttgat gcttctgttg
      661 ttgcgcttct aaaagcatca gtgtactgta atttcctagg catgtacttt tcgtctatac
      721 ctaatatctc gagaatatct tgcatatctt ctcgtttaat tagcatagac cctaatgaca
      781 tgtagagcaa ttgccctaat atcatctcgt ttttactctg atcgccattg atagcaatta
      841 gttctttaaa atccattatt aatacctccc ttgggattat ttgttatttg ttctaaaatt
      901 gttattagat tttttgaaaa tgggttaatt aaaagaattg agtttgaata ccaaaaaagg
      961 cctagagaat ataaatttcc ctagacctta atttcttgtt aaaacctaaa tatccatgaa
     1021 aaagatattt taataactta aatactatta cttatttaag tatatatcat ctatttcgtt
     1081 atagaaaggg tttttttgtt ttccacaact taccttttcc atgctgtaat cacgttgtaa
     1141 tccatgttgt aattttatac aatattttct gcttttatat tgacatttgt tgtcatttaa
     1201 tatatatttt atatatgatt tatatttatt ttaaatatgc tttacatttg ttattattta
     1261 tttgtttgga ggagggataa tttgtataca tttttacatt atccgattac tgtttgggaa
     1321 ctagctaccg agattaaaag agtatttaat gattattatt tacgacgtat cgatgaaaag
     1381 caattacatg cttgtatctt aacctgggtt gataactgcc ctaacaaatt gtttaaggat
     1441 gatgagataa atccaacagt taaagctgtt ataggaaaaa atagagagtt tgtattaaaa
     1501 aaaattataa ataacctttg tgatagttct attgctcaat agttttattt tttttatatg
     1561 gggatggtat taaaaacttt atgagggtat ttgcatgaat caagaagcac tagaattaaa
     1621 agatatgtta aggatcgagg gtataaattc taaaggtttt gggattattc caaaagtagt
     1681 aatgtttgac ccagatctat ctatcgaagc aaaaactata tatgcttact tttgtagtct
     1741 cgcaggtaat ggaacaacgg ctttcccaaa ccgcgatact attttgtttt atctaaatat
     1801 aacgaaagat tattactata agcactttaa tcaacttaaa gaacaggaat ttataaaggt
     1861 agaacgcaat agtaactttc cttataataa catttataca ctcattaata atcctaaaaa
     1921 atatgaagaa aaaatgtcaa actttgataa gaacaagcct tatagtcaat tgaaatacag
     1981 tggattaaag gcacatggtt tcggtattgt accaaggatt attatgatgg atagacgatt
     2041 acatattaaa gcaaaaggca tatatgctta tttttgtagt ctaataggca atggtattac
     2101 agctttccca caaataaatg aaatactttt ttatttgcat ataggaagac aagcttacta
     2161 caaatactat aaccaacttg tttcctgcaa ttatataaca gtagtacaaa gaaaaacaga
     2221 tagtggtaca ttttctgtta atgactatta tcttaacgat aatccggatt ataatattgg
     2281 tcaacaaaga caaggcgaaa aacaggagaa tctaccgtgt gatgatttac aggacaacga
     2341 caaatgtatt gataatacag gatttcaacc gtgtgatgat ttaccggaca acgatctacc
     2401 gtgtgatgat ttaccggaca acgatttacc gtacgacgat ttaccgtacg acgatttacc
     2461 agacaacaat aatataaaca gtattaatat aaacagtatt aatataaaca aatcaatcaa
     2521 tcaatcaaat gacaaacaaa aagctcttag aaagtcaact aagaaaaaaa tgattgataa
     2581 gattgataaa aataaaaaaa caaataaaaa agaattgcct acatatcaga atgattatga
     2641 actttataag aagcttatag cacaaaatat atcatatgaa gacttaaaac taaagttctt
     2701 taatgaaaat atgattggcg aaatattaaa tctaatgaca gagattgtta ccagcgacaa
     2761 gaagtacata cgtgtcaatg gagaggacaa gcctgcggaa atagttaaat caacgttttt
     2821 aaaattaaac gctacacata ttgagtatgt gcttgaaagt attgaaaata atactactga
     2881 gattaacaac ataaaagcat atatgataag tactttatat aacgcaccta tgacaataaa
     2941 tacatattat agaagccgtg tcaattatga tttatatggg agtaagtaga gtagaagcat
     3001 tacaagtaat acattatgca atacaatgta atgattacgt aatacaatat gcttttaggt
     3061 tcaaagaaat agcagattaa ttgctgaata gatgagtaag agttagaaaa ttacttttta
     3121 tgagccaaaa aattacacaa tataattatt tataaatatt aattgtaaaa ggaggttcta
     3181 tgtatatacc tgggtttaga tcgaaaaaaa caacgaagat aactatagca ataatttact
     3241 atgttttcag ccttttaatg ttgtttgtgg gcatttctta ctccctattt cttatatcac
     3301 taccatttat agttttttac ggtattgatt tacttaaata caagcaaaaa aagctgttag
     3361 ttccgtttat agcttcggta ttagtctttg tcgtatcaat aataatattt ccaacgaata
     3421 cccctaaaat agatgcagat agtaatacaa gtaatacatc cggtattact caagcagaca
     3481 aatcaataag tgataatgaa ccgactaata ccactgtagc tagtgaagat gaacctgata
     3541 ttgcgaaaga gaataataaa aatgttgttg ttgatgaagg tactgctaat gaagaaagca
     3601 ttgctaataa gggcgataca ggaactactc taaaagagaa tattactgat aaggataagg
     3661 ataaaacaaa ccctacagct actgtacagg atgaatcaaa aaaagaagtt tccggaaata
     3721 taaaagtcca ttttcttgat gttggccagg cagactgcat tttgatacaa acttctaaca
     3781 acaaaaatat gttaatagac gcaggtaata atgatgacag tagttttatt ttatcgtatt
     3841 taaacaattt agggatagag aagatagata ttgcagtagg cacccatccg catgaggacc
     3901 atataggaag tcttgatgat gttatcaaaa cgtttggtat tggcaaagta tacatgccca
     3961 aggtagaaca tagctctcag acttatcttg acgtgcttac ggcaataaaa ggaaaagaat
     4021 tgaaagttac tactcctgta gcaggagagt atattgattt tgatatagat acaaaggtac
     4081 aaattcttgc cccaaacagt gataaatatg ataatttaaa tgactattct atagttgtaa
     4141 ggcttgagta tggtaataca agttttcttt ttacaggaga tgcagaaact gtttctgaac
     4201 aagagattgt atcgaaaaaa tataatatta attctgatgt cctgaaggtt ggtcaccatg
     4261 gaagcacaac ttcttcatcc cttgccttta ttaaagcagt aagccctaaa tacagtgtga
     4321 taagcgtcgg caaggataac aaatatgggc atcctgatag tataattatt aataggttta
     4381 agatgttcgg tgaagtattg cgtactgacg aatcagggac tattattatt acatctgatg
     4441 gtgagaagat aacgatagaa aaaaaggctt cttccattaa ggaaaatgcc cctcccgaag
     4501 aatcacaaga ggttaataaa cctgctcctt ctacacagga agaaccttca aagcaggaag
     4561 gaatatatat aggtaacata aactccaaga tttttcatag ggaaacctgt accacattac
     4621 cagatcagaa gaaccaggtt aagtttaaca atagggaaga agcaatagat gccggttaca
     4681 cagggtgcaa aaggtgtaat ccgtgaaatt gataaggatg gtgtatatga agttaataat
     4741 agaccgattt gaaggagaat atgccgtttg tgaaaatgaa aaacggaaaa ttattaacat
     4801 tgagaaatca aaattgccta ctgcggccaa ggaaggcgat actattatta aagaaaatgg
     4861 ggaatatatt attgatgtag atgatagcaa aaacagaaaa gaaactatta cgaatcttat
     4921 ggatgagttg tttgactgat tatttattga gtagagaagg atgaacagta atggataagt
     4981 ttgttaaatg gcaggatgct aaagcttcaa tggcatcttt aagcgaagaa gagaaacagg
     5041 aaattgacat tatgtctgat ataataactg agattattga aagacggcaa gaacttgga
//
LOCUS       DAOSDD010000286         5080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5857_length_5080_cov_5.201393, whole genome shotgun sequence.
ACCESSION   DAOSDD010000286 DAOSDD010000000
VERSION     DAOSDD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_5080_cov_5.201393"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1970)
                     /locus_tag="PK733_17580"
     CDS             complement(147..1970)
                     /locus_tag="PK733_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12378.1"
                     /translation="MGFGPGGMMRGGEKARDFKGTMGKLIKYLSVYWTSIIVVLLFAA
                     ASAVFSILGPKILGKATTKLFEGIMEKIAGTGNGVDFKYIGGIILTLLGLYLLSALFS
                     YIQGWIMSGVSMKVSYSFRKDISEKISRMPLKYFESTNQGEILSRVTNDVDTVSHTLN
                     QSLTQIITSITTVIGVLVMMFSISWLMTLIALCILPISMMLVSFFIKHSQKYFREQQA
                     YLGNVNDHVEEMYGGHLVMKAFNGEEKSIERFDELNNNLYGTAWKSQFLSGMMMPVMN
                     FVGNIGYVAVSVLGGWLAVRKAIEVGDIQSFIIYVRSFTQPIAQLANISNVLQQTAAA
                     AERVFEFLAEEEEIPETTYPVKLEDIEGSVEFRNVKFGYNPDNTVINNFYAVIKPGQR
                     VAIVGPTGAGKTTMVKLLMRFYDVNEGAIFVDGHDIREFNRNDLRAMFGMVLQDTWLY
                     NGTIMENIRYGRPDATDEEVIAAAKAAHADNFVRTLPNGYNMILNEEASNVSQGQKQL
                     LTIARAILADPKILILDEATSSVDTRTEIQIQKAMNKLMENRTSFIIAHRLSTIRDAD
                     LILVMNHGDIVEQGTHKELLERGRFYSELYYSQFDVSQQVS"
     gene            complement(2090..4324)
                     /locus_tag="PK733_17585"
     CDS             complement(2090..4324)
                     /locus_tag="PK733_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12379.1"
                     /translation="MYKLMKYLKPFTGMIVAAIFLLFIQAMCDLALPDYMSNIVNKGI
                     QQSGIVNAVPKAVRQSTMDKLVLFMNPDNKIEVISNYTLIDDSNIDYSKYVREYPKLA
                     EEPIYILNNNIDESEVDKINPVMGKAILAVSGMEKAKASAKDGVISFNGMNIPANTDL
                     FELLTKLPKEQVLLINEAINEKFKDMDVNMITQMAVVSVKEEYAALGVNMDKIQNGYI
                     LRTGMYMLLISLLAAACTVIVGFLSSRTAAGLSRDLRKEVFTKVENFSNAEFDKFSTA
                     SLITRTTNDITQIQMLIIIMIRMVFYAPIMGVGGVIRAIGKSTSMSWIIAVAVAVLLG
                     LIILVFQVAMPKFKVIQKLIDKLNLVTRENLSGMMVIRAFNNQKFEEKRFDKANRDLT
                     NTSLFVNRVMACMFPVMTLIMNGVTLLIVWVGAHQIADSSMQVGDMMAFMQYAMQIIM
                     SFLMMSMMFIMIPRASVSAQRIAEVLETESVIQDPKIPKHFQDNVSGRGRVEFKNVCF
                     RYPGAEEDALKDISFVALPGQTTAFIGSTGAGKTTLVNLILRLYDVTRGKILIGDVDV
                     RDVTQNELRSRIGYVPQKSSLFSGTIESNLKYAVENAADEDIKKSAEIAQVIDFINEK
                     PEGVKSEISQGGTNVSGGQKQRLSIARALVKKPDIYIFDDSFSALDFKTDAALRKALK
                     SQTGTSTVLIVAQRISTIMNAEQIIVLDNGKIAGIGTHRELMDNCRTYQEIAYSQLSK
                     EELA"
     gene            complement(4317..4772)
                     /locus_tag="PK733_17590"
     CDS             complement(4317..4772)
                     /locus_tag="PK733_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ12380.1"
                     /translation="MEEISKSISVIRLLKEIMYAVRQNVEYQFKEMRLTGPQGMIIGI
                     LANFGEMKISDLSTKIGLSNSTVSGIIDRLEKQNLVERIRSKEDRRVVYVRVSAEFKR
                     NAKEHYNEITKKFEAMMNKATPEELDAIIDGLDKLKTVIDRQKGETENV"
BASE COUNT         1493 a         1138 c          787 g         1662 t
ORIGIN      
        1 ctgaagtgaa tattatacat ggaaatcgct tcttgccata tttcccctca aatatggaaa
       61 atgcaccctg gatgcacccg ggtgcaccta ttcagtccac ctgtgtattt attcggctaa
      121 cctgtggatt tatttagctc acctgtttag ctcacctgct gggaaacatc aaactgactg
      181 tagtaaagtt cactgtagaa gcgtcccctt tcaaggagct ccttgtgagt accctgttct
      241 acaatatcgc catggttcat aaccaggatt aagtcagcat cacggattgt tgacaaccgg
      301 tgcgcaatta taaagcttgt gcggttctcc atgagtttat tcattgcctt ttgaatctgt
      361 atctcggtgc gggtatcaac agaacttgta gcttcatcca gaataagaat cttaggatca
      421 gccagtatag cccttgcaat agttaaaagc tgcttctggc cttgtgatac gtttgaagcc
      481 tcctcattta aaatcatatt gtaaccgtta ggaagagtac gcacgaagtt atcagcatgt
      541 gctgcttttg cagctgcaat gacttcttca tctgttgcat caggacggcc ataacgtatg
      601 ttttccatta ttgttccgtt atatagccat gtgtcctgca aaaccatacc aaacatagct
      661 cttaaatcat tacggttaaa ttcccttata tcatgaccat caacaaaaat agcaccttca
      721 tttacatcat agaaacgcat taggagctta accatagtag ttttccctgc acctgtcggt
      781 ccgacaattg caactctctg accaggctta ataacagcat aaaaattgtt aattacggta
      841 ttatcaggat tatacccgaa tttcacattt ctaaactcaa cactgccctc aatatcttcc
      901 agtttaacag ggtaagttgt ttccggaatt tcctcctcct ctgccagaaa ctcaaataca
      961 cgctctgctg ctgccgcagt ttgctgaagg acattagaaa tgtttgcaag ctgcgcaata
     1021 ggctgtgtaa atgagcgcac atatatgatg aaagactgaa tatcaccaac ttctatagct
     1081 ttcctaacag caagccatcc gcccaaaaca gatactgcta cataaccaat atttcccaca
     1141 aagttcataa caggcatcat catccctgac aggaactgag acttccatgc tgtaccatag
     1201 agattattgt tcagttcatc aaaccgttca atactctttt cctctccatt aaaagctttc
     1261 attaccaaat gcccgccgta catttcctcc acatggtcat tgacattacc caaatatgcc
     1321 tgctgttccc taaaatactt ctgagagtgt tttataaaaa aacttacaag catcatggat
     1381 attggcagga tacatagtgc tataagtgtc ataagccagc ttatactgaa catcatgact
     1441 aaaactccaa ttactgttgt aatggatgta ataatctgag ttaagctctg gttcagggta
     1501 tgacttacag tatcaacatc attagttacc cgggatagaa tttccccctg attggtactt
     1561 tcaaaatatt tgagaggcat acggctgatt ttctcagaaa tatcttttct gaaactatag
     1621 cttaccttca tagaaacccc ggacataatc caaccctgaa tataactaaa taatgcactt
     1681 aataaataca aacccaaaag tgtaagtata attcctccaa tatatttaaa gtctacacca
     1741 ttacctgtac ccgctatttt ttccattatc ccttcaaaca gcttcgtagt ggctttgcct
     1801 aaaattttgg gtcctagtat agagaataca gcacttgctg cagcaaataa caatacaaca
     1861 attattgaag tccaataaac acttaggtat tttattagct ttcccatagt acctttaaaa
     1921 tcacgagcct tttcgccgcc tctcatcata ccaccgggtc caaaccccat accacgcgca
     1981 tcacgccgct ttgttgcctg tctttgtgtg catggggacg cttctccatt ttttatacac
     2041 ggggacgctt ctccattttt tatcttattt tccatattat ttcctaagct catgccaatt
     2101 cctccttcga aagctgtgaa taagcaattt cctggtatgt tctgcagtta tccataagtt
     2161 ctctgtgggt acctatgcca gctattttgc cattatcaag aacgattatc tgttcagcat
     2221 tcataatagt tgaaatacgc tgtgcaacaa taagtacagt gctagtgcct gtttgacttt
     2281 taagagcttt tcttaatgct gcgtcagttt taaagtcaag tgctgaaaag ctatcatcaa
     2341 atatatagat atcaggcttc tttactaaag cgcgggcaat tgataatcgt tgcttctgac
     2401 ctcctgacac atttgtacca ccctgagaaa tctcactctt aaccccctca ggcttttcat
     2461 taataaaatc aataacttga gcaatttcag ctgatttttt aatatcttca tctgctgcat
     2521 tttcaactgc atacttaaga ttagattcga tagtaccact gaacaatgag cttttctgag
     2581 gtacataacc aatcctggac ctaagttcat tctgcgtcac atctctcaca tcaacatcac
     2641 caataagtat ttttcctctt gttacatcat ataagcgtag gattaaattg accagagtcg
     2701 ttttacctgc gccggtagaa cctataaaag cggtggtctg tcccggcaaa gccacaaaac
     2761 ttatatcttt tagtgcatct tcctcagctc ccgggtacct gaaacataca tttttaaatt
     2821 ctacacggcc gcgtcccgat acgttgtcct ggaaatgctt cggtatcttt ggatcctgga
     2881 ttacagattc tgtttccaga acttcagcaa tacgctgtgc tgatacggaa gcccttggta
     2941 tcataataaa catcatagac atcattaaga atgacataat tatctgcatt gcatactgca
     3001 taaatgccat catatcacct acctgcatac tcgagtcagc gatctgatgt gcacctaccc
     3061 agactataag cagtgtaact ccgttcataa taagtgtcat aacaggaaac atacatgcca
     3121 ttacgcgatt tacaaaaagg cttgtatttg tgagatctct gtttgcttta tcaaatcttt
     3181 tttcctcaaa cttctggtta ttaaaagctc ttattaccat catacctgaa agattctcac
     3241 gggtaaccag attaagtttg tcaataagct tttgaataac cttaaatttg ggcatggcaa
     3301 cttgaaacac aagtatgatt agccctaaaa ggaccgcaac tgcaactgca ataatccatg
     3361 acatggaagt gcttttgccg attgctctga ttaccccgcc aacacccatt ataggggcat
     3421 aaaagaccat tcttatcatt attataataa gcatctgaat ttgagtaata tcattagtag
     3481 ttctagttat taacgaagca gtggaaaact tgtcaaactc cgcattagaa aaattctcaa
     3541 cttttgtaaa tacctctttg cgcaaatccc ttgataatcc cgctgctgtt cttgatgata
     3601 aaaatccaac aataactgta caagctgcag caagcaaaga tattagcagc atatacattc
     3661 ctgttcttag aatatatcca ttttgaattt tatccatatt aacacctaga gcagcatact
     3721 cctccttaac tgaaacaaca gccatctgag taatcatatt tacatccatg tccttaaatt
     3781 tttcatttat cgcttcattt attagcaaaa cctgctcttt cggcagcttt gtcaatagtt
     3841 caaaaagatc cgtatttgca ggaatgttca ttccattgaa ggatatcacc ccatcctttg
     3901 cagaggcttt tgctttttcc attccggaaa ctgccagaat agctttaccc attacagggt
     3961 ttattttgtc tacttccgat tcatcaatat tgttattcaa gatgtaaata ggttcttcag
     4021 caagtttggg atactccctt acgtacttgc tataatctat atttgaatca tcaatcaagg
     4081 tataattact tataacctct atcttattat ccgggttcat aaataataca agcttatcca
     4141 tagtgctttg cctaactgct ttcggtacag cgttcacaat accgctctgt tgtatcccct
     4201 tgttcacaat atttgacatg taatccggta gtgcaaggtc gcacatagcc tgaataaaaa
     4261 gcagaaatat tgcagcaaca atcattcccg taaacggttt taagtacttc atcaatttat
     4321 acattttctg tctccccctt ttgcctgtct attactgttt tcaatttatc aagtccatca
     4381 ataattgcat ccaattcttc aggagttgct ttattcatca tagcttcaaa ctttttcgta
     4441 atttcattgt aatgctcctt ggcatttctc ttgaactctg cagatacacg aacataaaca
     4501 acccttctat cttccttgct cctgattctc tcaaccaggt tttgtttctc caatctgtca
     4561 ataattccag atacagtact gttggataat ccgattttag tacttaaatc gcttatcttc
     4621 atttcaccga aatttgctaa aatccctata atcataccct gaggaccagt taatcgcatt
     4681 tctttaaact gatactctac attctgcctc actgcataca taatttcttt caataatcta
     4741 ataacgctaa tacttttgct aatttcttcc atgattgcac tcgctttcgc aaaacataat
     4801 atttcttaga aaatgttgtt tgactgtatt atatatagat acatttggag taaatacatt
     4861 tggtgtaaat atatttcgta taaatatatt tcgtgtacat acattttcta tagatatgtt
     4921 ttgtatacaa aatatattat agtacctgtt gctttttatg tcaataatag tgaagccatt
     4981 gggtgcatat tttatttttg aagaaattat tggaagaagc attaatgtag gtactgtagc
     5041 tgggcagggg aaactcttct ctcaagtaac agatattcct
//
LOCUS       DAOSDD010000287         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5859_length_5077_cov_5.134010, whole genome shotgun sequence.
ACCESSION   DAOSDD010000287 DAOSDD010000000
VERSION     DAOSDD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5859_length_5077_cov_5.134010"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1382
                     /gene="dinB"
                     /locus_tag="PK733_17595"
     CDS             96..1382
                     /gene="dinB"
                     /locus_tag="PK733_17595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ12381.1"
                     /translation="MKKVIFLVDMNAFFISCEMTRHPELIGKPAAVAGDPKKRTGIIL
                     AANYEAREYGVKTTMILQEAFRRCPNMRIVPPDHNFYEQKSKEVMSILSSYTPVLEQN
                     SIDEAWLDMTGCEGLFGEPLECAGRIMENIKAELGLWCSIGISENKFLAKMASELKKP
                     LGITELWEKDVQQKLWPLPVRFMYGVGKQTAKKLQVMGINTIGDLALFSQNILIKKFG
                     KSGIEMYAHANGIDDSGVIPKNNDDMKSIGRSLTLAEDISDIEKAKIILMQLADEVGM
                     LARKHEKKGRTVQITLKYADFKTITRQKTISPTYLTKEIYSTGINLLEHNWNIHKPVR
                     LIGISLCDFEENIDLKQISIMDLVAESAVAPTSNYKNGICMNNKEERLGEKERLWKKE
                     MLEKEERLEKTIDILKQKHGYSKITRAVLIEKKEKK"
     gene            complement(1603..3654)
                     /locus_tag="PK733_17600"
     CDS             complement(1603..3654)
                     /locus_tag="PK733_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12382.1"
                     /translation="MPLYPGNIVFCCNPNNDLYTALKDSEYEFKRDTNLENLIKEAEN
                     ETGILILADYYPRQTVEIGQNILNMAEAKNLRLYIEYPLSLEGLNLGEPSRTKYERLV
                     VSSDFFKSKLEKDTIMMLNGCWYLPVTGVEPHLCMAKAAGYSKIVYGLPEKVFPILFQ
                     MKENENILVSSSCLSNFITARYAPYVYWKNLWEYLLGWISRGEYTFNLKWEPGVGPTY
                     KKNDVAPADLEISALQRSFSWFRNHAVYSVDPKKGAIEGFESGIDHMGRQMIRNVTRS
                     DCVSESAMVLALDWHLNKDPDKKYLSKEILDYVWSPVFFQGDPNSPMYGLNNWYENGP
                     VFYGDDNARVIMATLLARSIIDTDRWDERVLKCLLANLRTTNKLGFRYNRLEYSSFLP
                     KDRSWSFYMNDDEYTSYSPHYQAYLWAAYIWAYSLTGYNQFLDISKNALRMCMEVYPE
                     KWYWTNSLTAEISRIILPLAFLVRVEDTQEYREWLYRAAGDLLKYMDESGAIQDMFGE
                     LKNGKYPPPQSNDNYGTTEASLIQNNGDPATDLLYTTNWAFLGLHEAAIATGDKLFKE
                     AEDRLAEFLCRIQVKSEYHKYLDGAWMRSFDFKKWEYWGSSADIGWGAWCVESGWVNS
                     WIACVLAMRHKRESIFNLAAADKFKKISPVLIEEMFTEKSLPLHGSNKGNFVSSMPGA
                     E"
     gene            complement(3660..4109)
                     /locus_tag="PK733_17605"
     CDS             complement(3660..4109)
                     /locus_tag="PK733_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12383.1"
                     /translation="MTIAPSSLEEVETALYKFTKNHPDKNSKYDTYGCSMSGLDLIYS
                     AFGYFPQSIFWHEKDGKVVPGLIQPEIKEALKLINRMYKDKVIDPEFVTGENHGGYWA
                     LTQLFINGRIGLSCFASFYHWGPGSKEKNYTDGGANWDALYKVKNKE"
     gene            complement(4435..4923)
                     /locus_tag="PK733_17610"
     CDS             complement(4435..4923)
                     /locus_tag="PK733_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HOJ12384.1"
                     /translation="MRFLIKQKIWTFGDDFTIKDEYGNEHFRVKGKVFALGDKLRMYT
                     MDGIELFYIEQKLFRFLPEYTIYHSGQPVATVKKEFTFFRPRFNISSTLGNFTIEGNF
                     WSMEFSILKSGIPVAQVSKKWFSWSDTYGVDIADNEDYAFILSLAIIIDQVIHDNNHN
                     NN"
BASE COUNT         1606 a          966 c          915 g         1590 t
ORIGIN      
        1 agtatttaaa ccagattata atatattata agattgcata tatgtttgaa tataagaccg
       61 aaaatatttt gaaagtgtta aatttattct ataatatgaa aaaggttatt tttcttgttg
      121 atatgaacgc ttttttcata tcatgcgaaa tgacaagaca tcctgagctt atagggaaac
      181 ctgctgcggt agcaggtgat cccaaaaaac ggacagggat tatcctggct gcaaattatg
      241 aagcaagaga atatggtgta aaaacaacca tgatattgca ggaagctttt agacgctgtc
      301 ccaatatgcg tatagtacct cctgatcaca atttttatga gcaaaaatca aaggaggtta
      361 tgagtatatt atcatcctat actcctgtct tagagcaaaa tagcatagat gaagcatggc
      421 ttgatatgac agggtgtgaa gggttattcg gtgaacctct tgaatgtgcc ggacggatta
      481 tggaaaacat taaagccgaa cttggtttat ggtgctctat tggcatatcc gaaaataagt
      541 ttttagctaa aatggcatct gaactgaaaa agccgctggg aattacagaa ttatgggaaa
      601 aagatgttca acagaaattg tggcccttgc ctgtccgatt tatgtatgga gtaggcaaac
      661 agacagctaa aaaattacaa gttatgggaa tcaatacaat tggggatttg gcacttttca
      721 gccagaatat tttaataaag aagttcggga aaagcggtat tgaaatgtac gctcatgcaa
      781 atggaattga tgattctggt gtaataccta aaaataatga tgatatgaaa tcgattgggc
      841 gttctttaac ccttgctgaa gacatatccg atattgaaaa agcaaaaatt atattaatgc
      901 agctggcaga tgaagttgga atgcttgccc gaaagcatga aaagaagggg aggacggttc
      961 aaataacact taaatatgca gattttaaga ctataacacg acagaaaaca ataagtccaa
     1021 cttatttaac aaaagagata tattctaccg ggataaattt gcttgaacat aattggaata
     1081 ttcataaacc tgtgcgttta attggaataa gcctttgtga ttttgaagaa aacattgacc
     1141 tgaagcaaat atccataatg gatttggtag ctgagtcagc agtagcgcca acttccaatt
     1201 acaagaatgg catttgcatg aataataaag aagagagatt gggggaaaaa gaaagattgt
     1261 ggaaaaaaga aatgttggaa aaagaagaaa gattggagaa aaccattgat atacttaaac
     1321 aaaaacatgg ttattcaaag attacaagag ctgtcttaat tgaaaagaaa gagaaaaaat
     1381 gatattcata tgaggatgat tctcactata attcctgaat tgacttaaaa ttacagcatc
     1441 atgaaaaaac ggaacaattc atgctttggt aggtgaaaat ggggctggaa agttatgcgt
     1501 aagcaggctc aggagatgat caacaacctg ggaatggata ttgatgtaga tgagcttgtt
     1561 caatgaactc aatgaaaacc gtccttgata actcatcgtc ttttactctg cgcctggcat
     1621 agaactaaca aagttgcctt tatttgaacc atgtagaggt aaagattttt ctgtaaacat
     1681 ctcttctatt agaacgggtg atattttttt aaatttatct gcggcagcca ggttgaagat
     1741 tgattctctt ttatggcgca ttgccagcac acatgcaatc caggagttta cccacccgga
     1801 ttcgacacac catgctcccc atccgatgtc ggcagagctg ccccagtatt cccacttttt
     1861 aaaatcaaaa ctgcgcatcc atgctccatc cagatatttg tggtattcag atttaacttg
     1921 tattctgcaa agaaattctg ccagcctatc ttcagcttct ttaaataact tatctccggt
     1981 tgcaattgct gcttcatgca atcctaaaaa tgcccagttt gtcgtataaa gcaggtcggt
     2041 agcagggtcg ccattatttt gtatgaggga agcctctgtt gtgccataat tatcattgga
     2101 ttgaggcgga ggatacttac cgtttttcag ttctccaaac atatcttgaa tagccccgct
     2161 ctcatccata tatttcaata aatctcctgc tgctctatat agccattctc tatattcctg
     2221 cgtgtcttcc actcttacaa ggaatgccag gggtaatatt attctggata tttcagctgt
     2281 cagactattt gtccagtacc acttttcagg gtatacttcc atgcacattc ttaatgcatt
     2341 tttagatata tctaggaact gattatatcc agtaagagaa tatgcccaaa tataagctgc
     2401 ccagagatat gcctgataat gaggggagta tgatgtatat tcatcgtcat tcatatagaa
     2461 agaccagctt ctgtcctttg gcaagaaaga actgtactca agtctattat aacgaaaacc
     2521 cagtttattg gttgttctta gatttgccaa aagacatttc agaactcttt catcccatct
     2581 gtctgtatct atgatgcttc ttgcaagtaa tgtagccatt attactcttg cattatcatc
     2641 accataaaat acaggaccat tttcgtacca gttatttaac ccatacattg gactattggg
     2701 gtcaccttgg aaaaacacag gagaccatac ataatccagg atttccttac ttagatactt
     2761 tttatcaggg tctttgttta aatgccaatc aagagccaaa accattgccg attcagagac
     2821 acagtcactt cttgtaacat tccttatcat ttgccttccc atgtgatcta tacccgactc
     2881 aaaaccttct atagcacctt ttttcgggtc aacactatat actgcatgat ttctgaacca
     2941 gctaaaggaa cgttgcaatg ccgagatttc aagatctgcg ggtgcaacat cattcttctt
     3001 ataagtagga ccaactccgg gttcccattt tagattaaaa gtatattccc ctctggaaat
     3061 ccatccgaga aggtattccc ataagttttt ccagtaaaca tagggggcat atcttgcagt
     3121 aataaaatta ctgagacaac ttgagctgac caaaatattt tcattttcct tcatttgaaa
     3181 aagtatgggg aatactttct ccgggagccc gtaaactatt ttgctgtatc ctgcagcttt
     3241 agccatacac aggtgtggtt ccacacctgt aacaggaaga taccagcaac cattcagcat
     3301 cattattgta tccttttcga gtttgctttt aaaaaagtct gaagagacca caagcctttc
     3361 atatttagtg cggctgggtt cacccaaatt taaaccttcc aggcttaaag gatattctat
     3421 atataacctt aagtttttgg cttcagccat gtttaatatg ttttgtccaa tttctactgt
     3481 ttgtcttggg taatagtctg ctagaatcaa tattcctgtt tcattctcag cttctttaat
     3541 caggttttct aagttggtat ctctcttaaa ttcatactct gaatccttta gggcagtata
     3601 tagatcatta tttggattgc agcagaatac aatatttcct ggatataacg gcacagtaat
     3661 cactccttat tttttacttt atatagtgca tcccagttag ctcctccgtc agtatagttt
     3721 ttttctttac ttccgggccc ccaatggtag aatgatgcaa aacaacttaa accaattctt
     3781 ccgtttataa atagctgtgt tagagcccaa tagcctccat ggttttcacc tgtaacgaat
     3841 tcaggatcga taaccttatc tttatacatc ctgttaatta gttttaatgc ttcttttatt
     3901 tcgggctgaa taagtcctgg aaccactttc ccatcttttt catgccagaa tatgctttga
     3961 gggaaatatc cgaaagctga atagattaag tcaagaccgg acatggagca cccatatgta
     4021 tcgtatttac tgtttttatc cggatgattc ttggtaaatt tgtacaaggc ggtttcaact
     4081 tcttcaagac tagaaggagc tattgtcata ccgatatttt ccagccaatc tcccctccag
     4141 gctatgggct gcctatatgg attccaaaag tttactcctg caagtccata taatttgcca
     4201 tcaagcttat tctcttccca gtgaattcaa ttgcaccgta ttactgtgta atgataaatg
     4261 tacggtacaa ccccaaaact atataaacta aagacgatta tttgactaat aaccgcacac
     4321 aataactaca cgcaatatcc gtacgcaata tctgcgtata atatctgtgc ataataacct
     4381 atatagttac tcgcatcatg acttcccaaa ttactatacc attactgtcc atcactaatt
     4441 attattatga ttattatcat gaataacctg gtcaataata attgcaaggg aaaggatgaa
     4501 ggcatagtcc tcattatctg caatatccac cccataagta tcgctccagg agaaccactt
     4561 tttagagacc tgtgctacag gtatgccgga ttttaatatt gaaaactcca tgctccagaa
     4621 attaccttca attgtgaagt tgcctaaagt acttgaaata ttgaatctgg gcctaaaaaa
     4681 tgtaaattcc ttttttacag ttgctaccgg ctgcccagaa tgatatatag tatattcagg
     4741 tagaaagcgg aatagtttct gctcaatata aaagagttcg ataccatcca tggtatacat
     4801 tcttagctta tcacccaggg caaaaacctt tccttttacc ctaaaatgct cattaccata
     4861 ttcatctttt atggtaaaat catcgccaaa agtccatatt ttctgtttta ttaaaaaacg
     4921 cataaaactg catcctccca gagttaaaaa atatttagtg aggggttcaa taattccatt
     4981 ataagggata tgggatgtgg cgtcaattga aaaatggtat tggggtgcat tttcttccta
     5041 tgatacaaaa tatggcagca aacgattttc cgagtat
//
LOCUS       DAOSDD010000288         5036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5921_length_5036_cov_3.670548, whole genome shotgun sequence.
ACCESSION   DAOSDD010000288 DAOSDD010000000
VERSION     DAOSDD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5921_length_5036_cov_3.670548"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..845
                     /locus_tag="PK733_17615"
     CDS             483..845
                     /locus_tag="PK733_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12385.1"
                     /translation="MVKEKLADDLQKVTEATAYPMTPTASDHIFQNKTRGLLEENSND
                     VYFRNLTYPIHTYDNYGNLIPLPLNPTPGTYEYNLKCDIVEAHLNDVSMYVEKLYDVT
                     SPDIVYLCSSYHQFSLLI"
     gene            complement(1890..4490)
                     /locus_tag="PK733_17620"
     CDS             complement(1890..4490)
                     /locus_tag="PK733_17620"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ12386.1"
                     /translation="MKGWVINSSLLILIVLALRYVLKGKISSRLRYALWIPVLLRLLI
                     PINFGGSAISIMNMFPSGNQTVGSTGESLGYISNVQTAFSATNEYNSSTLDSSQNDTY
                     NMNPNLNTNPNPGTLDKKFSWIFVAKTIWLAGIVIFGMSFLISNVVFFCRLKKSRIKA
                     DIDGYPLTIYNTDSVPTPCMYGLLRPAIYVTSEVLGNEIVLRYVLEHETTHWQHKDHI
                     WSLLRCMCLALHWYNPLVWVAAIYSIQDAELSCDEATIKRIGEVDRTEYGRTLIGLTC
                     AKRSANTLLVAATTMTGSKKSIKERITLIAKKPKMAIYTLIAVLLIAAIAVGCTFTGA
                     KGKKDKIVPLTDKEIQQYNKVFETISFDEQDNVIINPFSHFFKSYYDRPEDIDLALFL
                     RYFPSEEDVTDKAEFRALMATENWPFGADMTMERMPVPIHKFPTETINRTLQKNMGIT
                     LDDLKSIETESLIYLKEYDAYYNFTSDFAAGTFYCTHGETQGDIIRLYNEFVTLTLKK
                     CDDGFLFVSHQRIEKETHDINKIKDFGIAVSIKPRGHIPEAVLDYALEFVTKQIDHYN
                     NIGKNPPSGSRTYTIDGAKIINLTKMNTGTASLVTDIQMWLLEYRLRPDNPNNVVLAG
                     GMIMEDMEGETWITEWGSTGQPYLVLAHNIEEDTWERICITNTDAIMFDYNTPEMLER
                     YGDKYTAAAMELYQKATTEQINEISEKDMLRYIEEEIYIPGDAKEYLEKRELSNPSLL
                     INDDLLPIYDKYKETLNDELLKGLRPLDILRLRNKTIEEEDFDVRVNLIIFPSEINRD
                     DFLETVRNDEITKENEKLLLEKYRQYNGKVIEIIVSEGKAYVVIQNDGFWRFEKTKHG
                     VWKLGWLSRQ"
     gene            complement(4487..4873)
                     /locus_tag="PK733_17625"
     CDS             complement(4487..4873)
                     /locus_tag="PK733_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ12387.1"
                     /translation="MANQNINLTPTEWNVMECLWKSAPCTGREASEYLSKHVGWSRST
                     TLTLLRRMTNKGIIKCEIIDGLKSYSPLVKREDAVMNETEDFLSRVYNGSISVMLSTI
                     TKKQTLTEAEINELYEILRKAGEGEK"
BASE COUNT         1476 a         1033 c          803 g         1724 t
ORIGIN      
        1 aatcaaacgc tctgtttcaa gaattttcat aatcttcagc tccattccta tcattacttg
       61 actcatatat gatgttgaac actcatgcat ttctctaaaa tgcaaaaacc ccacagaatc
      121 acttttatgt gactccgtgg ggtaaattat ccacgtgtaa ggcttctata cccccatgca
      181 gctcaagcgt aagctcttat gcatgctcac cttttacagt atttttgcat atcttttcca
      241 tcttgtcaat aggttttttt cgagtttccc cattttttct taattcgctt aaaattaagt
      301 ttttaaagat aaaaacctaa gaaattatcc atagagctac tgaccttttg aatgctaatc
      361 cacagattgg aagtgaagat atttagctaa ttatgtaata tatttgttcc caaaaataag
      421 aaagacaata ggagatattt ggctcacaga tatttttgtt gtatatataa attaatacga
      481 ttatggtaaa agagaaactg gcagatgact tgcaaaaagt gactgaagca actgcatacc
      541 ctatgacccc aactgcttct gatcatatat ttcaaaataa aacaagggga ttgctcgaag
      601 aaaatagtaa tgatgtgtat tttcgtaatt taacatatcc aatacacaca tatgacaact
      661 atggcaactt aatacctctt cctttaaatc ctactccagg tacttatgag tacaacctta
      721 aatgtgatat agttgaagct caccttaatg atgtatctat gtatgtagaa aaactatatg
      781 atgtaaccag tcccgatatt gtatatttat gttcttctta tcatcagttt tcattactta
      841 tttgacacta ccttttattt tataaattat cttcatcttt aatgcaatcc tgcacatgcc
      901 ttaaaaatct tctttataga ctataaacct ctttttgcac ataatctaac tatactatac
      961 ttttgcataa atactgaaca ttgacaaata aagaaaatgg ctgttaacag cctaaacaaa
     1021 gaatagcatc cttgagggta gtattttccg ctacttccgc ctctaaagcg gtattggaga
     1081 aaacaatttt tcccatggaa aaccttctca ggcaaggaca taatctttta aagcccaaat
     1141 cataagtcca aggggtatcc cctccatatt aacgcccggc actcgtgacg gtttttagcc
     1201 ctagatagct cctatcttag gctgaaagct gtgagcacgc aacttcgtac tcatactcca
     1261 gagatctact tcatgttctg ccctttatgt gggtgctttg ttctctgtag ccacacatcc
     1321 cataactcgg agggcaacaa gacgtcactc ccttaataaa attccctaat attcactaca
     1381 cattatttaa tggtagtgag ccaaattaac ttcgcattat gcttatttgg ttttgctagg
     1441 agctatagac aaaagtaaaa tctaagcaaa tctgaccaca atatatagta ttggataagg
     1501 caacgaacac aacatatgcc ggattattat gcaatattga gacttttgtc caaggctcct
     1561 aggagcctgc tcggaaactc atcctaaatt tttacatgct agctccaaac ttgcatgaat
     1621 gctggattta gcatataatt ccatatcgtt tgtaaaaata taacaacctt ttatttaatg
     1681 cggagttaag tcatgtttgt taattattgg gagcactttt cgagtaggct cctaggttaa
     1741 tggttggtag aatattttaa ccggaaattt tggctgtata cgtgattgtt tttcttcttc
     1801 agctacctta agatatactc caatggtttc ctttacacat tatcattttt gttttttctt
     1861 gccttcagaa attatgcact aactatagcc tattgtcttg atagccagcc aagtttccaa
     1921 accccatgtt tagttttttc aaatctccaa aacccatcat tttgaataac aacataagcc
     1981 ttgccttcac tgacaattat ttcaataact ttcccattat actgtctata tttctctaaa
     2041 agtaattttt cattttcttt tgtaatttca tcatttctca ctgtttccag aaagtcatct
     2101 cgattaatct ccgatggaaa aataattagg tttactctta cgtcaaaatc ttcttcttcg
     2161 atagttttat tacgtaatct tagtatatcc aaaggtcgca aacctttaag caattcatca
     2221 ttaagagttt ctttatattt atcgtatatt ggcagcagat catcatttat tagcaaactg
     2281 ggattggata attccctttt ttcaagatac tcttttgcat ctccgggaat atatatttct
     2341 tcttcaatat accggagcat atctttttca cttatttcat taatttgctc agttgttgct
     2401 ttttgatata gctccatagc cgctgcggtg tatttatccc cataacgctc caacatttca
     2461 ggggtattat agtcaaacat tatcgcatct gtattggtta tacaaattct ttcccatgta
     2521 tcctcttcaa tattatgtgc caataccaaa taaggttgac cagtgctccc ccattctgta
     2581 atccaggttt ctccttccat atcctccatt atcatacctc cggcaagtac aacattattc
     2641 ggattatcag ggcgtaaacg atattccagc aaccacatct ggatatcggt aacaagactt
     2701 gcagtgcctg tgttcatttt tgtaaggtta atgatttttg ctccgtcaat ggtataagtt
     2761 ctgctaccgg atggaggatt tttaccgata ttgttgtaat ggtctatttg ttttgtaaca
     2821 aattccaatg cgtaatccaa aacagcttca ggaatatgtc cccttggctt tatactaaca
     2881 gctataccaa aatcctttat cttgttaata tcatgggttt ctttttcaat acgctgatgg
     2941 gatacaaata ggaaaccatc atcacatttt tttagtgtta acgttacaaa ttcattatat
     3001 aaacgaatga tatccccttg agtttcacca tgggtacagt aaaaagtacc ggccgcaaaa
     3061 tcactggtaa agttatagta ggcatcatat tcttttagat atatgagact ttcagtctct
     3121 atacttttta aatcgtctag ggtaattccc atattctttt gtaatgtcct gtttatagtc
     3181 tctgtaggaa acttatggat gggaacaggc atacgttcca tagtcatatc tgcaccaaaa
     3241 ggccagtttt ccgtcgccat aagtgcccta aattcggctt tatccgtaac atcctcttct
     3301 gatgggaaat aacgcaggaa taaagcaaga tcaatatcct caggcctgtc gtaataggat
     3361 ttaaaaaaat ggctaaatgg attgatgata acattatctt gttcatcaaa gctaatcgtt
     3421 tcaaagactt tgttgtattg ctgaatctcc ttatctgtaa gaggaacaat tttatctttt
     3481 ttacccttcg ccccggtaaa ggtacagcct acggcaatcg ctgcgattag tagtacggca
     3541 attagagtat aaatagccat tttcggtttt tttgctatta atgttatgcg ttctttaata
     3601 ctctttttac ttccggtcat agtggtggcc gccactaaca gcgtattcgc actgcgtttc
     3661 gcacaagtaa gtccaatgag tgtacgtcca tattccgtgc ggtcgacttc tcctatgcgt
     3721 ttgatcgtgg cttcatcgca ggatagttct gcgtcttgta tagaatatat agcagccacc
     3781 cacaccagtg gattatacca gtgcagagcg aggcacatgc accgaagcag cgaccagatg
     3841 tgatctttat gttgccagtg tgtcgtttca tgctccaata cataacgcaa cactatttca
     3901 ttgcccaata cctccgaggt aacgtagatt gccgggcgca acagtccata catgcagggt
     3961 gttggtacag aatcggtgtt ataaattgta aggggatatc catctatatc agctttgatc
     4021 cttgattttt ttaatctaca gaaaaataca acattggata tcagaaagga cataccaaaa
     4081 ataacaatac cggcaagcca aattgttttc gcaacaaata tccagctgaa tttcttatcc
     4141 aatgtacccg gatttggatt tgtatttaga tttggattca tattatatgt atcattttga
     4201 ctcgaatcga gagtgctgct attatattca ttagttgcag agaatgctgt ctgcacattg
     4261 cttatatacc ccaatgattc accggtactc cctacagttt ggttacctga cggaaacata
     4321 ttcattatac ttatggcgct tcccccaaaa ttgattggta taagcaagcg taaaagtacc
     4381 ggaatccata aagcataccg caatcgtgaa ctaattttac ctttgagtac atatcgcagt
     4441 gctaaaacta tcaaaattag caaagaggaa tttataaccc agcccttcat ttttctccct
     4501 cccctgcttt acgcaagatt tcatatagct catttatttc agcttcagta agggtttgtt
     4561 tttttgtaat tgtgcttaac attacactaa tactgccgtt ataaacacgg cttaggaaat
     4621 cctctgtctc attcataaca gcatcttccc gttttactaa aggggaatag gacttaagcc
     4681 catcaattat ttcacatttt atgatgccct tgttagtcat cctccgcagt agtgttaatg
     4741 ttgtactgcg agaccatcca acatgcttac taaggtattc actagcttca cgtcctgtgc
     4801 atggtgcaga tttccataga cattccataa cattccactc tgtaggtgtt aagttaatat
     4861 tttgattagc catagtattt cgcccctcct gtttacattg taacattatt atattttata
     4921 atatttacat tgtcaacagt aattactgtt taagcgcatt ttccataggc gtaaacccgt
     4981 ctttggaaaa attctctgcc gtagtataag aggaagacct ccattgagtt ttttct
//
LOCUS       DAOSDD010000289         5021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5940_length_5021_cov_3.424889, whole genome shotgun sequence.
ACCESSION   DAOSDD010000289 DAOSDD010000000
VERSION     DAOSDD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_5021_cov_3.424889"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK733_17630"
     CDS             complement(<1..535)
                     /locus_tag="PK733_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12388.1"
                     /translation="MPIIATLHKTLLDSGESLLFEKDRSDLITIVSNITFNILKDSPE
                     LVADVANGVVDRQVLETAIIKDIDENKYHYGIFRDELIKKVLDFMFGYGELQQYIENE
                     EITDIDGTKYNEFVVKKKGVREKVSINFGNEKIFDTYCKLIVIRNGGILNENDSHCRV
                     TDEKRRLRINVSIKPRNI"
     gene            complement(540..2258)
                     /locus_tag="PK733_17635"
     CDS             complement(540..2258)
                     /locus_tag="PK733_17635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOJ12389.1"
                     /translation="MKNTEKMADKNFGVKELLGGRYRLVKILGRGGMSTVFLAENINL
                     GTFWAVKKISKNVDVTKELPVESNILKKLNHPALPRIIDIIENEDNIYIVQDFIEGTS
                     LDKEIEKSGKFTETKVIEWAKQICDVLIYLHSFKPNPIIYRDMKPSNIILTPWGDIKL
                     VDFGIAREYKEDVKNDTVYIGTRGYAAPEQYGRGQSNVTTDIYNFGVTLFHLLTGEEP
                     GRFPFGLKLPECYNGNVSHRMMQIIYKCTRYNSEERYQSAHELKKDIEGWEKTSNVID
                     IVITDDTLHIGKLILTIWGNTEFACELAYIASKMTQLNILLVDLDLLSPSVDLFLNIK
                     KHSEGLVGEGILNESGINIVLNSIEKGYFTREDLLRACIKRKDLKNLYILTGNYRLEN
                     YEYYNNEGLIKLIEKSSQIFDILILAVNKSIYDSFTILCLIKSDLNIIPIRADIDKFR
                     EFNNYIAFLKEKQQIPMDKTKFVAFDYCPSWNLDKGTIEEIVQHNYLGKVGYCPRRAK
                     YRNLKIPYARKMDQDIIKDYISIIQKLKILHEGTYNKNRGVINTICRKIYKSFSKQRK
                     AKVLRR"
     gene            complement(2538..3017)
                     /locus_tag="PK733_17640"
     CDS             complement(2538..3017)
                     /locus_tag="PK733_17640"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOJ12390.1"
                     /translation="MTSEEYLKSMVEILKKKYDLLKEILDLSRSQTYAITDEEFETLE
                     KLIHGKQTVINCIEPLDERFNALFLELKTRLDIKSMEQLNNKNIHGKKDLKEATSKIL
                     EIVAEISLIEKQNNEMMQKLMKKSKKEIKNIVQGKKALSAYISHPFNSPSYYIDKKK"
     gene            complement(3070..3489)
                     /gene="fliS"
                     /locus_tag="PK733_17645"
     CDS             complement(3070..3489)
                     /gene="fliS"
                     /locus_tag="PK733_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOJ12391.1"
                     /translation="MALNNSYSKGYNQYRENTVFTSTPEELTLMLYNGIVKFIMQAQA
                     AIDENHIEKANSSIIKAQEIICELQLTLDMKYEISKRFMLLYDYIYRQLMEANIKKDK
                     NILEEVLGFAKELRDTWMQAMKAAKQQNNIREAETGV"
     gene            complement(3581..>5021)
                     /gene="fliD"
                     /locus_tag="PK733_17650"
     CDS             complement(3581..>5021)
                     /gene="fliD"
                     /locus_tag="PK733_17650"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ12392.1"
                     /translation="ESTIANVAEDLQNKIDAAFGVGKVIVEGVIKEIDGESFAELAFD
                     TIGGASRITLTNGDTNDGLSHLKIDSGSSNRLNTNQTLETLSSRFAQQLTFDDNGNLR
                     FFINDKEFTFSKTTTLLNMINTIDSDKGSNVSIIYDEALDKFRITAKQTGAGETIKIS
                     QGGGNFFDGVSGISAASPVTKSGKDAQVIIDGHIITRNSNNFTVNGINYTLHKVSTET
                     QKITVKPDVDGIYNSINSFVDKYNELIDKINGKLAEKYDRNYQPLTQQQKDAMGEKDV
                     EKWEERAKTGLIQNDSLLENIVFNMRRALFDKISGVDINLTSIGITTGLYHEKGKLKI
                     DESKLKEAIINNPDAVMDLFNKKSETHTSYNRTLNTQQRTVRYEESGLAQRIFDIIED
                     NITAIRDSNGKKGILLEKAGIEEDASEFSNIIFNEIEMYNLKIDTLYDKLIEKENRYF
                     QKFAVMERIIGQMNTQSNWLMAQLSQYNQ"
BASE COUNT         1417 a         1001 c          658 g         1945 t
ORIGIN      
        1 atatatttct tggttttatc gaaacattta tccgtagccg ccttttttca tcagttaccc
       61 tgcagtggct atcattttca ttaagaattc ctccgtttct tatcactata agcttgcaat
      121 atgtgtcaaa tattttttca tttccaaagt ttattgaaac cttttcacgt actccctttt
      181 tcttaactac aaactcgtta tattttgtgc catctatatc tgtaatttcc tcattttcaa
      241 tatactgttg aagctcccca taaccgaaca taaagtccag taccttttta atcagttcat
      301 ccctaaaaat tccataatgg tatttatttt catcaatgtc tttgattata gcggtttcca
      361 gcacttgcct gtcaaccaca ccatttgcaa catcagctac aagttcagga gaatccttta
      421 ggatattaaa agtaatatta gatacaattg taatcaaatc actcctgtcc ttttcaaaca
      481 aaaggctttc cccgctatca agcaatgttt tatgcaaagt agctataata ggcaaatttt
      541 cacctcctta aaacctttgc ttttctttgt tttgagaagc ttttgtatat cttacggcat
      601 atggtgttaa ttacccctct atttttatta taagttccct catgcagtat ttttaatttt
      661 tgaattatag atatataatc ctttattata tcctgatcca tcttccttgc ataaggtatt
      721 tttaagtttc tgtattttgc tcttctagga caatatccta ctttcccaag ataattatgt
      781 tgtacaattt cttcaatagt acctttatcc aaattccatg atggacaata gtcaaaggct
      841 acgaatttcg ttttatccat agggatttgc tgcttttctt ttaaaaatgc aatataattg
      901 ttgaattccc taaacttatc gatatcagca cgtatgggaa ttatattcaa atcggatttt
      961 ataagacata aaatagtgaa cgaatcgtaa attgacttat taacagctaa tattaaaata
     1021 tcgaaaattt gacttgattt ctcaataagc tttataagac cttcattatt gtaatattcg
     1081 tagttttcaa gccggtaatt tccggttaga atatataaat tcttaaggtc cttacgtttt
     1141 atacatgctc taagaaggtc ctccctggta aagtaacctt tttctatgga gttcagcaca
     1201 atgtttatac ctgattcatt taatatccct tctcctacca acccttcaga atgttttttt
     1261 atattcagaa acaaatctac actgggagat aataagtcca aatcaaccaa cagtatgttt
     1321 aattgtgtca ttttcgatgc aatatatgcc agctcacatg caaattcggt attgccccat
     1381 attgtcagaa taagttttcc gatatgcaaa gtatcatccg ttataacaat atcaattaca
     1441 tttgatgttt tctcccaacc ttcaatatct ttttttaatt catgtgcaga ttggtatctt
     1501 tcttcagaat tatatctggt acacttatag attatttgca tcatccggtg agatacattt
     1561 ccgttataac actctgggag ctttagccca aaaggaaacc ggccaggctc ttcacctgta
     1621 agcaaatgaa aaagagttac cccgaagttg tatatatccg tagttacatt tgactgacct
     1681 ctcccgtatt gttcaggagc agcataacct cttgtaccta tgtaaactgt atcgtttttt
     1741 acatcctctt tatattccct tgctatacca aagtctacta attttatatc tccccatggt
     1801 gtaagaataa tatttgaagg cttcatgtct ctataaataa ttgggttagg tttaaaggaa
     1861 tgaaggtata tcaaaacatc acaaatctgt ttagcccatt caattacctt agtctctgta
     1921 aatttacctg atttttctat ctctttatcc agagaggttc cttcaataaa atcctgaaca
     1981 atatatatat tgtcttcatt ttcgataata tctataatcc ttgggagtgc agggtggttg
     2041 agtttcttta atatatttga ctcaacaggc agttctttag tcacatcaac attttttgat
     2101 atttttttta cagcccaaaa ggtaccaaga ttaatatttt ctgcaagaaa tacagtgctc
     2161 atgcctcctc ttcccaatat tttaacaagc ctgtatcttc ctccaagaag ctcttttact
     2221 ccaaaatttt tatcagccat tttttccgtg tttttcatat cgccttggca ggaaactaat
     2281 atacctttag gagatacatt tgcatacccc gctatacttt tgggggatgt atttatatac
     2341 cccgccctgc ctttttcacc acctagtcaa aattactggt aatttgtatc tgtaccatcc
     2401 tctttatcat gttatatttt gtactatgcc atataattta tcatatatgt atgttatttc
     2461 caattataca taatataaca ttatttgtca acatatatta taatgtttat atgaaatatt
     2521 tgccttttta ttttttttta ttttttcttg tcgatatagt aagatgggga attgaaagga
     2581 tgagatatat aggctgataa tgccttctta ccttgaacaa tatttttaat ttccttcttg
     2641 cttttcttca ttagtttttg catcatctca ttgttttgct tttctattag gcttatttct
     2701 gcaacgatct caagtatttt tgaagttgct tccttaagat cctttttccc atgaatattc
     2761 ttattgttca gctgttccat acttttaata tccagtctgg tctttagctc caggaaaaga
     2821 gcattaaacc gttcatccaa cggttcaata caattaatta cagtttgttt tccatgtata
     2881 agtttttcaa gtgtttcaaa ctcttcatca gttatggcat atgtttgaga tctggataaa
     2941 tccaatattt cctttaataa gtcatatttt ttcttcaata tttctaccat actttttaga
     3001 tattcctcag aagtcatata accacctctt ttgaaattct aactctacta cccaactttg
     3061 tcaaccggct caaacacctg tttcagcttc ccttatgttg ttttgctgtt ttgctgcctt
     3121 cattgcctgc atccaggtat ccctaagttc ttttgcaaag ccaagtacct cttcaaggat
     3181 atttttatct ttctttatat tggcttccat aagctgccgg tatatataat catataaaag
     3241 cataaatctt ttggatatct catatttcat atccagggtt aactgaagct cacatataat
     3301 ttcttgagct tttattatac tactatttgc cttctcaata tggttttcat caatcgcagc
     3361 ctgtgcctgc ataatgaatt tcacaatccc gttatagagc attaatgtaa gttcttcagg
     3421 agttgatgta aaaacagtat tctctctata ttggttatat cccttactat atgaattatt
     3481 taaagccata atgttagcct cccataaata aagtctgcta tatgctgctg cactccgctg
     3541 ttgatacagt ctgcacaact ttatcccctg attgatatct ttactggtta tattgagata
     3601 attgtgccat caaccaatta ctctgtgtat tcatttgccc aataatacgt tccattactg
     3661 caaatttttg aaaatacctg ttctcctttt caataagttt gtcatataaa gtatctattt
     3721 ttaaattgta catttctatt tcattaaaaa taatatttga aaattccgaa gcatcttctt
     3781 ctatacccgc cttctccaat aagataccct ttttaccgtt tgagtccctt attgcagtaa
     3841 tattatcttc aatgatatca aaaatccttt gggcaagccc ggattcctcg tatcttacag
     3901 tccgctgttg agtattaaga gttcgattgt atgaagtatg agtttcagat tttttattga
     3961 aaagatccat aactgcatca gggttattta taatagcctc tttgagcttt gattcatcaa
     4021 ttttcaattt ccctttttca tgatataatc ctgtagttat ccctatgctc gtaagattaa
     4081 tatccactcc gctaatttta tcaaataatg ctcttctcat attgaacacg atattttcaa
     4141 ggaggctgtc attttgaatt aaccctgttt ttgcccgttc ctcccacttc tcaacatcct
     4201 tttcacccat agcatccttt tgctgctgtg ttaatggttg atagtttcta tcatattttt
     4261 cagccagctt tccatttatt ttatcaatta gttcattata cttatctaca aaactgttaa
     4321 tgctattata tataccatcc acatcgggtt ttaccgtaat tttctgagtt tcggtactta
     4381 ccttgtgtaa tgtataattt ataccattaa cagtaaagtt attgctgttt cttgttataa
     4441 tatggccatc tatgataacc tgtgcatctt tgccgctttt agtaacaggt gaagctgcac
     4501 ttattcctga tacaccatca aaaaagttgc cgcccccctg acttattttt atagtttcac
     4561 ctgctcccgt ctgcttggca gttattctga atttatctaa cgcttcatca tatataatgc
     4621 tgacatttga acccttatca ctatctattg tatttatcat atttaataaa gtggttgttt
     4681 ttgaaaaagt aaactcttta tcattaataa aaaatctcaa attgccattg tcatcaaaag
     4741 tcagttgttg agcaaaccta gaagagagag tctccaaggt ttgattggtg ttaagcctgt
     4801 tgcttgagcc tgaatcaatt ttcaaatgag acaacccatc gttggtatcc ccatttgtca
     4861 aggtaattct gcttgctccc cctattgtat caaaagctaa ttctgcaaaa gattctccat
     4921 caatttcttt aataactcct tcaacaatta ctttaccaac tccaaaagct gcatcaatct
     4981 tattttgtaa atcctctgcc acattagcaa tcgtactttc t
//
LOCUS       DAOSDD010000290         5011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5955_length_5011_cov_5.571832, whole genome shotgun sequence.
ACCESSION   DAOSDD010000290 DAOSDD010000000
VERSION     DAOSDD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5955_length_5011_cov_5.571832"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK733_17655"
     CDS             <1..204
                     /locus_tag="PK733_17655"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ12393.1"
                     /translation="AAEIAERMRNSIQNKTFIYEGKRFRITASFGICTFSGNACITIE
                     DFIESADKKLYDAKKEGRNRVKF"
     gene            complement(259..939)
                     /locus_tag="PK733_17660"
     CDS             complement(259..939)
                     /locus_tag="PK733_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12394.1"
                     /translation="MGSIYVDFDQDVPRFACTYCNNCTSIMGKSLCNISNRGCCWYFP
                     KFTLLDIQRMIRTLDGIQTLEHIKNQPGTVINNYYIHAKGFFDKEGYDKWLSDNFFED
                     KPVEDCTIFFRACPFVKKGSGCTLPPRFRTIVCNFFICNDVIGRADIQGAFKPYIEER
                     SRYAAWVEWENQTLEHLLREKGVDLARNYEASISILQDTPLDVYEFPMLEPINMDDTW
                     NDTWNKGA"
     gene            1251..2480
                     /locus_tag="PK733_17665"
     CDS             1251..2480
                     /locus_tag="PK733_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12395.1"
                     /translation="MKLLIIGAGSRGRVYANYAKQYGAGIVAVADPNEERLKMFAERY
                     SIPADGLYKSWEEALKGDKYADAVIISTPDKVHYGPAMAALEKGYHILLEKPMSPNEQ
                     ECRDMVNKAEEKGLLLMVCHVLRYSPFFEKLKEVLESGRIGQIINFQLTENVVYWHFA
                     HSFVRGIFRNETVSSPWILAKSCHDLDIITYLMGRKCKSVTSKGNLMYFKKENAPEGA
                     TDYCLGGCPHEKTCPYFAPALYLKQITNVTWPSDVISVDTSVAARYEALKTGPYGRCV
                     YKCDNDVVDHQSAIFEMDDGSTATFNMIGVSSENTRTMRIFGTKGDIRGHLEKGQIEV
                     CNFLTGKKELIDIDYTTIVSGHGGGDARLLKDFLDAISGKPAEQKTSARISIQSHLMA
                     FAAEKSRKEGVAVNIEY"
     gene            2587..3414
                     /locus_tag="PK733_17670"
     CDS             2587..3414
                     /locus_tag="PK733_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOJ12396.1"
                     /translation="MIVNSTEFQNNFGKYLMLVAKEDVIITKNGREAAKLTTVDYSPG
                     RVNYMDGAVCEETAGYLFNNRKATYEEFLELSKDSQYRYEYIDGEIYLLASPRTSHQL
                     AMTEILRVFITWFEGKKCIPIAAPYDLTLWRDDDRIETDTQSNSESNNVSRSESANES
                     NIKPDIKPNIVQPDIMIICDLEEKLNENDYYMGVPSLVVEIVSESTRRRDYVKKLDVY
                     MSCGVQEYWIVNPLNKSITIYCFKDRNISDTATFKNGEIAESYIFDGLGVELVRVFR"
     gene            3649..>5011
                     /locus_tag="PK733_17675"
     CDS             3649..>5011
                     /locus_tag="PK733_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12397.1"
                     /translation="MKKIIAFFYSKSMFVQLMSSFLLVILVVSSFQLITNSIYRYQFE
                     QELINNTYKRFDYITSKTNQEFESIILGVLVNLNQEFGNSLRSGNISTIEVDKIKKKM
                     LDYVAQNNLIENIIIINSKDNTVLDYNGKYNKDIYFNKNMSNENYGWKYWLNQLGEDY
                     MFKILPVDNFTEFSNFGWVDDKTKALIPIIYKEKKGSNFCIIAFIDAVKFERKIEPDF
                     LNGFSIYSLSDGKTIYPATDKNNINIEVIKKDLKSPDGILNKKEGLSFVKKVNFTSLA
                     FYKYSPNEMLREYMNKTNTLFNIIIIISIALGIILSVLIIKSFNNPVKQIMELIKQSN
                     NMSAIPNITNMSVRSGENMDIINLRNITNTVKNLVEKNKHYENDINKKDSILKSFYYR
                     EKLLNMFSGTKEVQNEFDSKHNSFSRYIVVAFKVHCREEFQNLVPAEYRNSIFVLNEL
                     IDRY"
BASE COUNT         1773 a          756 c          933 g         1549 t
ORIGIN      
        1 gctgcagaaa ttgctgagag aatgcgtaat tctatacaga acaaaacttt tatatatgaa
       61 gggaagcgtt ttagaataac tgcaagtttt ggtatttgta cttttagtgg gaatgcatgt
      121 ataaccatag aagactttat tgaaagtgca gataaaaaac tttatgatgc aaagaaagaa
      181 gggcggaaca gggttaaatt ctgagcaggt gccgacacca aaatatatcg gcacctattt
      241 atatcgcact aatatctatc aggcaccctt attccatgta tcattccatg tatcatccat
      301 attaattggc tcgagcatgg gaaattcata gacatcaaga ggtgtgtcct gaagtatgct
      361 gattgatgct tcatagttcc ttgcaagatc aacacctttt tcacgaagca aatgttccaa
      421 agtttgattt tcccattcca cccatgcggc gtagcgtgaa cgttcttcaa tataaggctt
      481 aaatgcaccc tgtatatcag ctctgccaat aacatcgttg catatgaaaa aattgcatac
      541 aattgtcctg aaacgtggtg gtagagtgca gccggaacct ttctttacaa aaggacatgc
      601 tctgaaaaat atagtacagt cctcaacagg tttatcttca aaaaagttat cacttagcca
      661 tttatcatat ccttccttat caaagaatcc ttttgcatgg atataataat tgttaatgac
      721 agttcctggt tggtttttta tatgttctaa cgtttgtatt ccgtccaggg ttcttatcat
      781 tctttgtata tctaaaagag taaacttggg gaaataccag cagcatcctc tattacttat
      841 attacacaga ctttttccca taatgcttgt acaattattg caataagtac aagcaaaacg
      901 gggcacatcc tgatcaaaat ccacatatat actgcccatc tattttttct ccttcttgaa
      961 taatgaaagc aaatgtcagt tataaaaatc atcaatatta taaattatat cttttgatat
     1021 tattatttag caaactccat attttttatt attgaactat tttactttaa ataacaaaac
     1081 tatggttatg ggaatttcta tagcttaggc ttgcgttatg tttttgaaat taaaaaggtg
     1141 taatattgca tctaaatcta ttgcagtaca ggtttatttg ttgtaactga tcgctgctat
     1201 atgttataat ttgtataaaa tatattcaat aattggaggt atcaattgat atgaaactgt
     1261 taataatagg ggctggttcc agaggcaggg tatatgccaa ttatgcaaaa caatacggtg
     1321 caggaattgt tgcagtagca gaccctaatg aagaacgttt gaaaatgttt gcagagagat
     1381 acagtattcc tgcagatgga ctatataaat catgggaaga agctttaaag ggtgataaat
     1441 atgctgatgc agtgattatt tcaacacctg ataaggttca ttatggacct gcaatggccg
     1501 cccttgaaaa aggctatcat atattgcttg agaagcccat gtcgccaaat gaacaagagt
     1561 gcagagatat ggttaacaag gctgaagaaa aaggcctgct gcttatggtc tgccatgtac
     1621 tgcgctattc acccttcttc gaaaaactca aggaagtact tgaaagtgga agaattggtc
     1681 aaataataaa ctttcagctt actgaaaatg tggtttactg gcactttgct cattcttttg
     1741 tgagaggcat attcagaaac gaaactgtat caagtccatg gatattagca aaatcctgcc
     1801 acgatctgga tataattaca tacctaatgg gacgcaagtg taaatctgta acttctaaag
     1861 gaaatcttat gtattttaaa aaggaaaatg cacctgaggg tgcgacagat tattgtcttg
     1921 gcgggtgccc tcatgagaaa acctgtccct attttgcacc ggctctttac ctgaagcaga
     1981 taaccaatgt aacatggcca tccgatgtta tatccgttga tacatccgtt gcagcacgat
     2041 atgaagctct aaagacagga ccctatggaa gatgtgtgta caaatgtgat aatgatgttg
     2101 tggaccatca gagtgcaata ttcgaaatgg atgatggttc tactgcaaca tttaatatga
     2161 taggggtatc aagtgaaaat acaagaacta tgagaatatt tggaactaaa ggtgatatca
     2221 gagggcatct cgagaaagga caaattgaag tttgcaattt tctcacagga aagaaggaac
     2281 ttattgatat tgattacact actatagtat ccggtcatgg cggtggtgat gcaaggcttc
     2341 ttaaagattt cttagatgcc atatccggaa aacctgcaga gcagaaaaca tcggcccgga
     2401 tttccattca aagccactta atggcctttg ctgcagaaaa gtcaagaaaa gaaggtgtag
     2461 cagtaaatat agagtattag aaacttagaa acagtggagt attgacaggg attactataa
     2521 gacatataat atgattagct atattagcta atatagctaa tcatattata taaacggggt
     2581 gtaactatga tcgtaaactc aacagaattc cagaataatt tcggaaagta tcttatgctt
     2641 gttgcaaaag aggatgtaat tattacaaaa aatggacggg aagcagctaa attaaccaca
     2701 gtggactata gtcccggaag agtaaattat atggatggag cagtatgtga ggaaacagcc
     2761 ggataccttt ttaacaacag aaaagcaact tatgaagaat ttcttgagct ttcaaaggat
     2821 agccagtaca ggtatgaata tattgatggt gaaatatatc tgctggcatc accaagaaca
     2881 tcacaccagt tagctatgac tgagatattg agagttttta ttacatggtt tgaagggaaa
     2941 aaatgtattc ctattgcagc accatatgat ctaacacttt ggagagatga cgacagaatc
     3001 gaaacagaca cccaatcaaa tagcgaatcc aacaatgtat cacgtagcga gtcagctaac
     3061 gaatcaaata ttaaaccgga tatcaaaccg aatattgtac agcctgatat tatgattata
     3121 tgtgatctgg aagaaaaact aaatgaaaat gattattata tgggtgttcc ttcccttgta
     3181 gttgagatag tatctgagag tacgcgaaga agggattacg taaaaaaact tgatgtatat
     3241 atgtcttgcg gcgttcagga atattggata gttaatcccc taaataagag tattacaatt
     3301 tattgtttta aagatagaaa tattagcgat acagccactt tcaaaaatgg tgaaatagct
     3361 gaatcatata tttttgatgg gctgggtgtg gagcttgtca gagttttcag atagcccctg
     3421 gtaaatattt cttcactttt agtacatgca cacttatttt gatttgttat tgaatttcat
     3481 catgaaatac tatataataa acaggtaaat gtataacagg atataacatg ttatatattt
     3541 acctatttta tacataacat tacagaattt tagcaataca taaatgtaac attattacac
     3601 cttaggaagg tataaactta agcggaacct ttattaaggg ggagcggaat gaaaaaaatt
     3661 atagcttttt tttattcaaa atcaatgttt gtgcagctta tgtcaagttt tttacttgta
     3721 atacttgttg tgtcctcttt tcagttaata acaaacagta tttacaggta tcagtttgaa
     3781 caagaattaa tcaataatac atataaaaga tttgattata ttacttcaaa aactaatcag
     3841 gagtttgaaa gcattattct aggggttctt gttaacctta atcaggaatt tggcaacagc
     3901 ttaagatcag gaaatatttc aactatagaa gtagataaaa taaagaaaaa aatgcttgat
     3961 tatgttgctc aaaataactt aattgagaac atcattatta ttaatagcaa agacaacacc
     4021 gttttggatt ataacgggaa atacaataaa gatatatact ttaacaaaaa tatgtcaaat
     4081 gagaattatg gttggaagta ttggctaaat cagttgggag aagattatat gttcaagata
     4141 cttcctgtgg acaactttac cgagttttct aatttcggat gggtggatga caagacaaaa
     4201 gctttgattc caataatata caaagagaag aaaggaagca atttttgtat tattgcattc
     4261 atagatgctg ttaaatttga aagaaaaatt gagcctgatt ttctcaatgg attttctatt
     4321 tatagcctta gtgatggaaa aacaatatat ccggcaacag ataaaaataa tataaatatc
     4381 gaagttataa agaaagactt aaaaagtccg gatgggattt tgaataaaaa ggaagggctt
     4441 tctttcgtaa agaaggtaaa ttttacatcc ctggcatttt ataaatattc accaaatgaa
     4501 atgttaagag aatatatgaa taagactaat acactattta atatcataat aattatttca
     4561 atcgcactgg gtatcatcct ttctgttcta attataaaaa gctttaataa tccggtaaag
     4621 cagattatgg aattaataaa gcaatcaaac aatatgtctg ctatacctaa tattaccaat
     4681 atgtcagtta ggtctggtga aaacatggac attataaacc ttagaaatat caccaacact
     4741 gtgaaaaacc ttgtagaaaa aaacaagcat tatgaaaatg atattaataa gaaagactct
     4801 attttaaaaa gcttttatta tagggaaaaa cttctgaata tgttttccgg tacaaaggaa
     4861 gtacaaaatg agtttgattc taaacataac agcttctcaa gatatattgt tgttgcattc
     4921 aaggtgcatt gcagagagga gtttcagaat ttagtgccgg ccgagtatag aaacagtata
     4981 tttgttttaa atgagttaat agataggtac a
//
LOCUS       DAOSDD010000291         5009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_5961_length_5009_cov_5.216795, whole genome shotgun sequence.
ACCESSION   DAOSDD010000291 DAOSDD010000000
VERSION     DAOSDD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5961_length_5009_cov_5.216795"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PK733_17680"
     CDS             <1..1282
                     /locus_tag="PK733_17680"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOJ12398.1"
                     /translation="VKFPRSVVKKCLETIPAKVKLLDRDGNTAVILGDGGRYCASGHN
                     AVFTMTDESGERREATQKDVEQFAIVSDYLEDVDVIGIPYSPQDVTAETALLYAIKII
                     LENSKKPIFYSCESDIINKAAIEMAKVVLDRESLRDGSNLISQLSTTSPLYWERGTVE
                     ALYVCAKEGIPIDFLPQPIAGVTAPYTLAGILTIHNTEVLSGIVITQLINPGTPLIYG
                     AAWTTYEMKLTSVLIGRPESSLLRIAGSQMAHYYNMPSHTTAPDNDSNLYDEQGAWEK
                     MLSTVAGIIGANDMIVNLGMFGTGMSISLEQLVMDSEICRIVKRFLKGFEVNKETIAL
                     DTIANVGIRNTYLMEDHTIANLRSGEHVELNVSNGANYDVWKGKGAKSSSEKSRDIIN
                     AILTKGNKCPLEQSLSKGLSNIIEKYEKQMELKG"
     gene            1289..2593
                     /locus_tag="PK733_17685"
     CDS             1289..2593
                     /locus_tag="PK733_17685"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12399.1"
                     /translation="MQPIIGLVSLAGEFEVGFYKTPVLLKIAGENLRKADFKIVKSDT
                     VMYNSKTMKVVVNDLKEKEIDILCVCVGTWSEDHHLLDLLEYIKKPVILWAFPAVDTG
                     SLCGVQQICCVLKELDKEYFYVYGEADDTAIIEEIGRIARAAALKNKLRHVKIGNIGG
                     RIKGMTEIAFDELEIKDRTGVRIVNMDEDELVEAFKLTDMKQAAEYWNNLKTKASKVS
                     STDEHGIESIRYYFAMKKLIEEYDLEGIAVKCYPKWMGKICLGYSILSEEGVVCGCEG
                     DVNNTVSMKILYELSNTPTHNADLLYPDTDLNTILFSHCGSGGFSIASSEKDIHLGPV
                     RLADTGVCLLFPAKPGKVTLINLVGRKGTLRMSAIIGEAVECGMEFPGNPMKVKFKKN
                     VDEINRMIANEGVGHHWIGGYGDMSKELEYFCTLNRIRFIKL"
     gene            2726..4666
                     /locus_tag="PK733_17690"
     CDS             2726..4666
                     /locus_tag="PK733_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ12400.1"
                     /translation="MIRKVSLNGQWDFVADLDPKYHGDTRIYPDPPYAKPDANRRHWI
                     KVMVPGVWQKYAERYDIYEGVCWFAREFKLNGYKKGTAARMRFGAVNYFCRIFLNGEE
                     IGQHETGYTEFVLDVTKQLKEGLNHIAIMVDNRATKTKWPPCLGYFNYGGIHRAVTLE
                     IMEGVCLDSISLKGCYDMDMSTCRLRVTGKVTGINKNKTNKTNGETNGDSPKTNGDSP
                     HNQNCDQNRGDSPQNKSNSSRKNKENCPHFTSTDILHVEISCGSEKTEAKINTDGIIE
                     GELILPDAKPWTPEEPNLYTVTVKLIERESVLDIYEVQYGFKSVKIQDGKIHLNGKPL
                     NLRGVCYVYDSTVTGLVMNLEQMETDVKLMKEMGCNAVRCHYPMDKQFYEVCDRRGIL
                     VWIEPTVYCYHPLDDETNTGFSNSEWMSLAQQMTVEMIRVARNHPSVTIYGIGNECNT
                     YNTEAQVFFEKLAKLIRNEDPARLISYAALYGIVGPVADMVDVLGINSYWGWYDKIFG
                     GKGLKPEGDEWQKESNVTIEPIDLEPMRKMLDKVLSECRKNLVLFLTEFGADSVPGFY
                     SKSRDLWSENYHADLLKEIFKLSDEYHQIAGTFPFCFSDYRDPSKVPNGYWNEINLKG
                     VVTYERRKKLAFDTLKHRYTTT"
     gene            4757..>5009
                     /locus_tag="PK733_17695"
     CDS             4757..>5009
                     /locus_tag="PK733_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12401.1"
                     /translation="MSIIVDYLRKTTLEFIKNQKLKNGEIGEYRYSSAVSKPTLYSSA
                     YAAMTCSLYHDLDLLTAEEKERWIIYFNLHQDDDGLFRDN"
BASE COUNT         1674 a          773 c         1177 g         1385 t
ORIGIN      
        1 agttaaattt cctcgaagtg ttgtaaagaa gtgcttggaa acaattcctg caaaggttaa
       61 gcttctggat cgggatggca ataccgctgt catacttgga gatggtggaa ggtactgtgc
      121 aagcggacac aatgccgtat ttaccatgac ggatgagtca ggagagagac gtgaggcaac
      181 acaaaaagat gtcgaacagt ttgctatagt atcagattat cttgaagatg tggatgtaat
      241 aggcatacca tacagtccac aggacgtaac agcagaaact gcactattgt atgctataaa
      301 aataatactt gaaaattcta aaaagccaat tttctattcc tgtgaaagtg atataattaa
      361 taaggctgcc atagagatgg caaaagttgt tttagatagg gaaagcctta gagatggttc
      421 aaatcttata agccagcttt ctacaacaag tcctctgtat tgggagagag gtactgtgga
      481 agctctgtat gtgtgcgcta aagaaggaat ccctattgat tttcttccac aacctattgc
      541 aggagtgact gccccataca ctctcgctgg tattcttaca attcataata ctgaagtgct
      601 ttcaggtata gttataacgc agctgattaa tccgggaact ccgcttatat atggtgcagc
      661 gtggacaaca tatgaaatga agcttacaag tgtactaata ggaagaccgg aatcgtcact
      721 tctaagaata gcaggttctc agatggctca ttattataac atgcccagtc acacaactgc
      781 ccctgacaat gactcaaacc tttatgatga gcagggagcg tgggaaaaga tgctaagtac
      841 tgtagcaggt attataggcg ccaatgatat gattgtaaat cttggtatgt ttggtacagg
      901 gatgtccatt agccttgaac agctggttat ggatagtgag atttgcagga ttgtaaaaag
      961 attccttaaa ggatttgagg taaataaaga aaccatagct ttagatacta ttgcaaatgt
     1021 aggtataaga aacacttacc tgatggaaga tcatactatt gcgaatctga gaagcggaga
     1081 acatgttgag cttaatgttt caaacggtgc aaactatgat gtttggaagg gcaaaggtgc
     1141 aaagagttct tcagagaaat ctcgtgatat tataaatgct attcttacta aaggtaataa
     1201 atgtccttta gagcagtcat tgagcaaagg actttctaac attatcgaaa agtatgaaaa
     1261 gcagatggag ttgaaagggt gatatacttt gcagccaata ataggattag tttcacttgc
     1321 aggtgaattc gaggtagggt tttataaaac tccggtgctc ttgaaaattg ccggagaaaa
     1381 cctaagaaaa gcagacttta aaatagtaaa gtcagataca gtaatgtata atagtaaaac
     1441 aatgaaagtt gttgtaaatg acttgaagga aaaggaaatt gatattcttt gtgtatgtgt
     1501 gggtacctgg agtgaagacc atcatttgct ggatcttctt gagtacataa aaaagccggt
     1561 tatactatgg gcatttccag ctgtagacac aggttctctt tgtggagtcc agcagatatg
     1621 ctgtgtcttg aaggagcttg acaaggaata tttttatgta tatggagaag ctgatgatac
     1681 ggcaatcatt gaggaaatag gacggattgc aagagctgca gcattaaaaa acaagcttag
     1741 gcatgtaaag ataggaaata tagggggcag aatcaaaggt atgacggaaa tagcctttga
     1801 tgaactggag ataaaggata ggactggggt tcggatagta aatatggatg aggatgagtt
     1861 ggttgaagcc tttaaattaa ctgacatgaa acaggctgcg gaatactgga ataatcttaa
     1921 aactaaagcc agtaaagtca gttctaccga tgagcacggg atagaatcaa tcaggtatta
     1981 ttttgcaatg aagaaactta tagaggaata tgacctggaa ggcattgcag ttaaatgtta
     2041 tcccaaatgg atgggtaaga tatgcctggg atattcaata ctttctgaag agggggtagt
     2101 gtgcgggtgt gaaggtgatg tcaataatac tgtatcaatg aagatacttt atgaactatc
     2161 taatacacct acacataatg ctgatttgtt gtatcctgac actgacctta atacaattct
     2221 gttttcacac tgcggttcag gaggtttctc aatagcgagc agtgagaagg acatacatct
     2281 cggaccagta cgactagctg atacaggtgt atgtttgttg tttcctgcca agccgggaaa
     2341 ggttaccctt ataaatctgg ttggaagaaa aggaacctta aggatgagcg caattattgg
     2401 agaagctgta gagtgtggta tggaatttcc cggaaaccca atgaaggtta aatttaaaaa
     2461 gaatgtagac gaaataaaca ggatgatagc aaatgaaggt gtgggacatc actggattgg
     2521 ggggtacggt gatatgtcaa aggaactgga gtatttctgc acgcttaaca ggataagatt
     2581 tatcaagctt taaattaaag gagcagttgc atgtaaatcc agccgtaaac ctcatcagca
     2641 tgctgtgggg ataaaggcat cacttcatac ttataacgcc cagcaattcc aaaaatgaat
     2701 gtgaaaaggc aaggaggtag acaagatgat aagaaaagtt agtctaaatg gacaatggga
     2761 ttttgtagcc gatctggatc ctaaatatca tggagataca agaatatatc ctgaccctcc
     2821 ctatgcaaaa ccggatgcaa accggaggca ttggataaag gtaatggttc ccggtgtatg
     2881 gcaaaaatat gctgaaagat atgatattta tgaaggagtc tgttggtttg cccgggaatt
     2941 taagttaaat gggtataaaa aaggaactgc tgctcgcatg cgatttggcg cggtaaacta
     3001 cttttgtcgg atatttctaa acggggagga gattgggcag catgaaacag gatataccga
     3061 gtttgtccta gatgtaacca aacaacttaa agaaggttta aatcatattg caattatggt
     3121 ggataaccgg gcgacgaaaa caaaatggcc gccatgcttg ggttatttta attatggtgg
     3181 tattcacaga gcggttaccc ttgaaattat ggaaggtgtc tgcctggata gtataagcct
     3241 taagggctgc tatgatatgg atatgagtac ctgcaggtta agagtaactg gaaaagtaac
     3301 cgggattaat aaaaataaaa ctaataaaac taatggtgaa actaatggag acagtccaaa
     3361 aactaatggg gacagtcctc ataatcaaaa ttgtgatcaa aatcgcgggg acagtcccca
     3421 aaataagagt aacagctccc gcaaaaataa ggagaactgt ccccatttta cttctacgga
     3481 tattttacat gtagaaattt catgtggcag tgaaaaaaca gaagcaaaaa taaatactga
     3541 tggtattatt gagggtgaac ttattcttcc agatgcaaaa ccctggactc cagaagagcc
     3601 aaatctttat actgtaacgg ttaaacttat agaaagagaa tctgtgttag atatttatga
     3661 ggtacagtat ggcttcaaga gtgtgaaaat acaggatggt aaaatccatc ttaacggaaa
     3721 acctttaaac ctgaggggtg tttgttatgt ttacgactct actgtaaccg gactggtgat
     3781 gaatttagaa cagatggaaa ctgatgtaaa gctaatgaag gagatgggct gtaacgcagt
     3841 aaggtgccat tatccaatgg acaaacagtt ttatgaagtc tgcgatagac gtgggatatt
     3901 agtatggatt gaacctactg tatattgtta ccatcctctg gatgatgaaa ccaacacagg
     3961 cttttcaaat tctgaatgga tgagtttggc tcagcagatg acagtagaaa tgatcagggt
     4021 tgcgaggaac catccaagtg taaccattta cggcatagga aatgagtgta atacatataa
     4081 tactgaagct caagtatttt ttgagaagct ggcgaaacta attcgtaatg aagaccctgc
     4141 aagattgata tcctatgctg cattgtatgg gatcgtaggc cccgttgcag atatggtgga
     4201 tgttttagga attaactctt actggggatg gtatgacaag atatttggag gaaagggctt
     4261 aaagccggaa ggtgatgaat ggcagaagga aagtaatgtt actatagagc ccatagacct
     4321 tgaacccatg aggaagatgc ttgacaaagt gctttcagaa tgccggaaga acctggttct
     4381 ttttcttaca gaatttggtg cggattcagt gcctggcttt tattctaaaa gcagggattt
     4441 gtggtctgag aattatcatg ctgatctttt aaaagaaatc tttaagctat cagatgagta
     4501 tcaccagatt gcagggacat tcccattctg tttttcagat tatcgtgatc cttctaaggt
     4561 acccaatgga tactggaatg agattaattt aaaaggtgta gtgacatatg aacgcagaaa
     4621 aaagctggcc tttgatacat tgaagcacag gtatacaact acataaaatg agcagttttt
     4681 acatgcttat tttgcaatat tgagactttt gtccaaagct gctagtatat aactacacaa
     4741 aaaaaggagt agtactgtga gcattatagt agattatctt cgtaaaacca cattggaatt
     4801 tattaaaaat cagaaattga aaaatggaga aattggagaa tatagatatt catcagctgt
     4861 ttccaagcct accctttatt cctctgctta tgccgcaatg acatgttctc tttatcatga
     4921 ccttgaccta ttgactgctg aagaaaaaga gaggtggatt atatatttta acctccacca
     4981 agatgatgat ggtttattca gagataatg
//
LOCUS       DAOSDD010000292         4964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6017_length_4964_cov_3.749847, whole genome shotgun sequence.
ACCESSION   DAOSDD010000292 DAOSDD010000000
VERSION     DAOSDD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6017_length_4964_cov_3.749847"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK733_17700"
     CDS             <1..219
                     /locus_tag="PK733_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HOJ12402.1"
                     /translation="AELEILNQEEAELIIYEGKFHQIKRMFESVGKKVKYLRRVAMGG
                     LKLDKNLEAGSFRELTEEEIGILKQCIK"
     gene            complement(340..1284)
                     /locus_tag="PK733_17705"
     CDS             complement(340..1284)
                     /locus_tag="PK733_17705"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ12403.1"
                     /translation="MRSRIKRKKLLTVEEKLKRYKRRFFVLLSILILFISLTGIYIYL
                     NYDYLVFKHFISQNYIYTKTLDKLYNDELKRDVKSRYFSYFDNVVISVVTKKIQEINS
                     DRYTYLYIPEQYKKYKEEEREEALLSELKILDNNTAYLHITNFSNYTRKFLEGNIEMI
                     KKYPGLIIDLRSNYGGDTLAMYAMTDLFLPKGSIISTDKMRLFSKTYKAKKNKILSNK
                     NIIILQDKNTASASENFIAGLRDNLENVLLIGDKTFGKGIGQFTMPLKRGFAVKATTM
                     LWYTPNGINIQGEGISPDIYYSQDDIIDFALRKINQLD"
     gene            complement(1468..1698)
                     /locus_tag="PK733_17710"
     CDS             complement(1468..1698)
                     /locus_tag="PK733_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOJ12404.1"
                     /translation="MNVKVYSTPTCPWCTVAKKYLASRNVAFEDLDVSADKNAAMEMV
                     QKSGQRGVPVIDIDGEIVVGFNQNAIDKLLKL"
     gene            complement(1945..3663)
                     /locus_tag="PK733_17715"
     CDS             complement(1945..3663)
                     /locus_tag="PK733_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ12405.1"
                     /translation="MKNIPLHDVRPITNVKDMINSSVEIYGDKAAFLVKNKSDNNNYS
                     PITYKQLKHDMDAMGTALLNLGLKDKRVAIISENRYEWAISYLAVLNGTGIVVPLDKE
                     LPQNEIENLVKRSKVNVIIFSDSIYPQIQEMAKGNISVEYYINMDGIEDNNGILAFNK
                     LIMEGKELLGKGDTSFTNADIDVKAMNILLFTSATTDKSKAVMLSHENLVTNLMSMSS
                     MICINPTDIFLSVLPIHHTYECTCGFLCPLYRGAAIAYCEGLRHIPKNLQESKATVML
                     AVPLIFEAIFRRIWDQAKKTGSDKKLKTGIKISNLLRKFGIDITRKLFAPVYSNLGGS
                     VRLFISGAAPIDANVSKGFRDLGLLFLQGYGLTECSPIAALNRDVDFKDNAAGLPLPG
                     LELKIDAPNDEGVGEIIVKGPNVMLGYYQDTESTSKFLIDGWFYTGDLGYIDNDGFVH
                     ITGRKKHVIVTKNGKNIYPEEIEILLNRSPYIKESLVYGKSDEVYGDVVVSASIVPDI
                     DRIQEIFKDQSPSDEEIYNLIKKEVKEVNKKLVIYKYIRDFNLRENEFAKTTTKKIKR
                     YMERVD"
     gene            complement(3844..4032)
                     /locus_tag="PK733_17720"
     CDS             complement(3844..4032)
                     /locus_tag="PK733_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ12406.1"
                     /translation="MADKTLICKDCNNEFVFTEGEQAFYKEKGFQNEPQRCPDCRKAR
                     KQQRRNNNFGSRKFGENR"
     gene            complement(4503..>4964)
                     /locus_tag="PK733_17725"
     CDS             complement(4503..>4964)
                     /locus_tag="PK733_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ12407.1"
                     /translation="IFIETADNEKEHAKRFYKFLLEGLKEELPAVVEINTAYPVMQGT
                     TLENLKAAANGENEEWSELYSDFAKVAKEEGFSEIASAFSMIASAEKRHEIRYQKLAE
                     NVASNKVFNKDNIILWKCGNCGYIHEGTSAPDICPACVHPQAFFEVFVEAY"
BASE COUNT         1537 a          928 c          742 g         1757 t
ORIGIN      
        1 gccgaattgg aaatactcaa tcaggaagaa gcggaactaa taatttatga aggcaaattt
       61 catcagatta aaaggatgtt cgaatcagta gggaagaaag tcaaatattt acgaagagtg
      121 gcaatgggag gacttaaact ggacaaaaac cttgaagcag gtagcttcag ggagttgacg
      181 gaagaggaaa taggaatttt aaagcagtgt atcaaatgat ggcaatatat ttatcatcta
      241 cgttctcatt tacattctta tctgtattta ttatttgcat atttgcacgg aaatctgaat
      301 ttattcatag cagactccca gagctattta tagtgatagt tagtccaact gattgatttt
      361 tcttaaggca aaatcgataa tgtcatcctg actataataa atatcgggac ttattccttc
      421 tccctgtata tttattccat tgggagtgta ccataacatg gtggtagctt taacagcaaa
      481 acctcttttt aaaggcatag tgaattgtcc gataccttta ccaaaggttt tatcacctat
      541 tagaagaaca ttctcaaggt tatcccttaa tcccgctata aagttttctg aagcacttgc
      601 tgtattttta tcctgaagga tgattatatt tttattggac aaaattttat tcttctttgc
      661 tttataagtt ttcgaaaaaa gccgcatttt atcggtagat attatactac ctttgggaag
      721 aaataaatct gtcatagcat acatcgccag ggtatcccca ccatagttgc ttcttaaatc
      781 aataataagg cccgggtatt tttttatcat ttcaatattc ccttcaagaa actttcgagt
      841 ataattagag aaatttgtta tatgaagata agctgtatta ttgtcaagaa ttttcaattc
      901 cgacagtaag gcttcctccc tttcttcctc tttatatttt ttatactgtt caggtatata
      961 aaggtatgtg taacggtcac tgtttatttc ctgtatcttt tttgtaacaa cagagattac
     1021 cacattatca aaataactaa aataccggct ctttacatcc ctttttaact catcattata
     1081 taacttgtcc agtgttttgg tatatatata gttttgggaa ataaaatgct tgaaaactaa
     1141 ataatcgtag tttaaatata tataaatacc agtaaggcta atgaataaaa ttaaaatgga
     1201 cagcagaaca aaaaatcttc ttttatatct tttcagtttc tcttcaacag ttaataattt
     1261 tttccgttta attctacttc tcattaactt aattctcctc taatgctaac atattttatt
     1321 tctatattac catgtaatat attataaaca caacagtcag aagcatatct taaagtagga
     1381 ttattagctc atttttacaa aattaatgga gggtgggtat taacctccct ccattaatta
     1441 attcatgaaa gtaaaacgat agctatttta taatttcaac aatttatcaa ttgcattctg
     1501 gttaaatcca actacaattt caccatctat atcgattacc ggcacccctc tctgaccgga
     1561 cttttgcacc atttccatag ctgcattctt atcagctgac acatctaagt cttcaaaagc
     1621 tacattcctt gaagcaagat actttttagc tactgtacac caagggcatg taggtgttga
     1681 atatacttta acattcataa aatgcctcct ccttaaacat catatttgct ttgcaaattc
     1741 attttgaact ataacagcca atgttgcatg gctgcacctg aaattagtat aactatatgg
     1801 tactaaacta atccaagttt ttatccagtt gttatataat ataccctgta tatatttatt
     1861 ataacataaa tgcgattaat gaaataaaac catggtggtt ttatctttat gcatttattg
     1921 taacgtaaat tttactgcaa gtgtttaatc tactctctcc atatagcgct taattttttt
     1981 tgttgtagtc tttgcaaatt cattttcccg cagattgaaa tccctaatat atttatatat
     2041 tacaagcttt ttattaactt ccttaacttc ttttttaatc aaattgtaaa tctcttcatc
     2101 ggagggagac tgatctttaa aaatctcctg tattctatca atatcaggaa ctattgaggc
     2161 tgagactacg acatcaccat aaacttcgtc gctttttcca taaaccaatg attccttaat
     2221 gtaagggctt ctatttaaaa gaatttcaat ttcttccgga taaatattct taccattttt
     2281 tgttactatt acatgtttct ttcttcctgt aatatgcaca aaaccatcat tgtcaatata
     2341 tcccagatcc ccggtataga accatccatc tattaagaat tttgatgttg attcagtatc
     2401 ctgataataa ccaagcataa cattaggacc tttaacaata atttcgccaa ctccttcgtc
     2461 atttggagca tctattttta gctccagccc gggaagaggt aaaccggctg cattatcctt
     2521 gaaatcaaca tccctgttaa gagctgctat gggactgcat tcagtgagtc catacccctg
     2581 aagaaataac aagcccaaat ccctgaaacc ttttgagaca ttagcatcta tgggagctgc
     2641 tccgcttata aacaatctta cactgccgcc taaattacta tatacaggtg caaatagttt
     2701 tcttgtaata tctataccga atttgcgcag caaattactt atttttattc ctgttttaag
     2761 ttttttatca gaaccggtct tctttgcttg atcccatatc ctcctaaata tagcttcaaa
     2821 aattagtggt actgctaaca ttactgtggc ttttgattcc tggaggtttt ttggtatatg
     2881 ccttaaacct tcacaataag caattgcagc tcccctgtat agaggacata gaaacccgca
     2941 tgtacattca tatgtatggt gtatgggtag taccgaaagg aaaatatcgg ttgggttaat
     3001 acatatcatt gagctcatag acataagatt agtgacaaga ttttcatgag acagcattac
     3061 tgctttggat ttatcagttg ttgcagaggt gaataaaagt atattcattg cttttacatc
     3121 aatgtctgca ttggtaaaac ttgtgtctcc tttacctaaa agctctttcc cttccataat
     3181 caatttatta aaggcaagta taccattatt gtcttcaata ccatccatat ttatgtagta
     3241 ttctacagag atatttcctt tagccatttc ttgtatttga ggatagatac tatcggaaaa
     3301 aataattaca tttaccttgg atcgtttaac aagattctca atctcatttt ggggcaactc
     3361 cttatccaaa ggcacaacta ttcctgtacc atttaaaact gcaagatatg atattgccca
     3421 ctcatacctg ttttcagaaa ttattgccac tcttttatct tttaaaccca aattcagaag
     3481 tgcagttccc attgcgtcca tatcatgctt taactgtttg taggttattg gagagtaatt
     3541 attattgtcg cttttattct ttactaaaaa tgcggctttg tcaccataaa tttctacact
     3601 tgaattgatc atgtctttta catttgtaat gggcctgaca tcatgaagag gaatattttt
     3661 catataacct tacaccccca aaatattaaa cattatataa taattatttt agtgcaaaaa
     3721 catattcaga gtcattttgt taaaatttga gttaatgcag tagaatcaat aataaattaa
     3781 ataagctgaa ttaaataaag gagagtgtac actctccttt atttaagctt ttattagtgc
     3841 attctaccta ttttcgccga actttcgtga accaaagtta ttattccttc tttgctgttt
     3901 ccttgctttt ctgcaatccg gacacctttg tggttcattt tggaagccct tttccttgta
     3961 aaaagcctgt tcaccttcag taaaaacaaa ttcgttatta cagtccttgc aaataagagt
     4021 cttatctgcc ataaaaacac ctcctaaaca attgggggat ttaattaact tgactattat
     4081 ctccaatttg tttaaggagg ataaggtcca caggttaatt aaatcttaac atatacagat
     4141 agattataac acggctatat tttatataca aggtattttc tgcaaaaatt tccgtaatat
     4201 ttcctatgtt tttcaaatgt ttgcagctat tcaggggtat agaacggagt acacacatgg
     4261 gaatgtatac acatgggcat gggggacata tacacatggg gacggttctt gtgtgtccgc
     4321 gtgtgttcca tgcgacaaat aatgtacatc cccatttcac aatgtatcaa gacgtatatg
     4381 atgcactggg aaccgtcccc agtgcatcac agtgcatcag tgcatcatta cccctaaaca
     4441 gttacctatg atttgtttag gaatcagaaa aatgaaaagc aaatttctat gcttatactt
     4501 tgctaatatg cttcaacgaa tacttcgaag aaagcttgtg gatgtacgca agctggacat
     4561 atatccggtg cacttgtacc ttcgtgtata taaccacagt ttccacattt ccagaggatt
     4621 atattatctt tattaaacac tttattgctg gcaacatttt cagcaagttt ttggtatctt
     4681 atctcatgcc ttttctctgc agacgcaatc atactaaacg ctgaggcaat ttcagagaat
     4741 ccttcttctt tggcaacttt tgcaaagtca gaataaagtt ccgaccattc ttcattttca
     4801 ccattagctg ctgctttaag gttttcaaga gttgtaccct gcataacagg atacgctgta
     4861 ttaatttcca cgacggcagg taattcctcc tttaaacctt ccagaagaaa tttgtaaaat
     4921 ctttttgcat gttccttttc attatcggct gtttcaataa aaat
//
LOCUS       DAOSDD010000293         4933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6061_length_4933_cov_3.396679, whole genome shotgun sequence.
ACCESSION   DAOSDD010000293 DAOSDD010000000
VERSION     DAOSDD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6061_length_4933_cov_3.396679"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK733_17730"
     CDS             complement(<1..664)
                     /locus_tag="PK733_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOJ12408.1"
                     /translation="MLGFVAIAAIASLGFAAYNHFSVKKLNTGTELMNEIAGAIHEGA
                     DAFIMHEFKVVLKIGILVAGALMIFVSWYTGMAFLIGATMSQLAAFVGMNIATIANVR
                     VSNTARESKSLGKTLKVAFQGGSVMGLSVGGFALLGLGLVYLVFGKMLGQTDVVNIKI
                     ETNWLGITYIPFAMSVSGYALGCSIIAMFNRVGGGIYTKAADMSADLVGKTEAHIPED
                     DPR"
     gene            complement(1339..4770)
                     /locus_tag="PK733_17735"
     CDS             complement(1339..4770)
                     /locus_tag="PK733_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOJ12409.1"
                     /translation="MNLNENAIKVLERRYLTKDEDGNLLEDPEGMFRRIAKAVAEADF
                     GYADSSQLKTIEDEFYKMMSELEFLPNSPTLMNAGRPLGQLSACFVLPVEDTMEGIFE
                     SIKNAALIHKSGGGTGFSFSRLRAKGSSVKSTGGVASGPVSFMKVFNAATEAVKQGGT
                     RRGANMGILRVDHPDIREFITCKQDNKDITNFNISVGITEKFMKAVENNEKYDLIDPK
                     TKLPIRQESAREIFDMIVDNAWNNGEPGIIFLDRLNKDNITPELGEIESTNPCFHPDT
                     LISTADGLISIKELYEKHGDSEFDIFVDNRVSGKTITRNYREYMINGVSKKKARVFKT
                     GVKKTVKITLSNGQELKVTNDHKILTLQGWKEAGQLSEDDYVLIQSGKGRFPEKDNIG
                     REMGLLLGRVTGDGWMTSDGDVVGLVFADEEYYIYEHIDQIACGYGAGCPKPHMRQNN
                     TWQVMYKKKEFIEKIKDYGLLPVKSPDKRVPKAIFTSSEETVKAYLNGLFSSDGTVNY
                     VDENHRDIRLSSASEYLLKDVQLLLLNFGISSSIYKREKINQKSFTYVDTAGNERTYT
                     GGIYYELIINGDDMYNFSKTIGDLIHRGKNAKLKKAAKISRKKIKFMSRVFAVEDFEE
                     VEVFDINEKDTHSLIAQGIVAHNCGEQPLLPYEACNLGSLNLSLMLKKVKKGFEVDFD
                     KLGSVVKKAVHFLDNVIDVNKYPLEEIDRMTKGTRKIGLGIMGWADMLCKMGISYNSQ
                     EAIDLAEKVMGFIQEESKKASMEIAERKGVFPFYDKSIYKDQGIKIRNATTTTIAPTG
                     TLSIIAGVSSGIEPIFAICYIRNVMDNDELVEVNPVFKEIAIKNGFYSDELMKSIAKK
                     STLKDFDEIPAEIQDIFVTSHDITPEWHVNMQATFQKYTDNAVSKTVNLRNEAGKDDV
                     KDVFELAYKTNCKGVTIYRDGSRDAQVLNIGSVKGKDEKKNTEGKKDTEGDKGLENPQ
                     GIIVKYVAPRARPDITTGFTEKVRIGCGNLYITVNYDEQGICEVFTNLGRGGGCPSQS
                     EATSRLISIALRSGIDVKSLVEQLKGIRCATTIRQKGLKVMSCPDAIGRVIEKVMQLQ
                     NNGNGKEEELQILSEYEDTDVENELKYVYCPDCGKKLEHEGGCVVCRNCGFSKCG"
BASE COUNT         1424 a         1075 c          650 g         1784 t
ORIGIN      
        1 ttcttgggtc atcttccggt atatgagcct ctgtttttcc taccagatca gcgctcatat
       61 cagctgcttt tgtatatatt ccaccgccaa cacgattaaa catggctata atagaacaac
      121 caagagcata tcctgaaact gacattgcaa agggtatata agttattccc aaccaattag
      181 tttcaatttt tatatttact acatcagttt gtccaagcat tttaccaaat accagatata
      241 ctaatccaag acctaataga gcaaacccac ctacagacaa acccataacg cttccacctt
      301 gaaaagccac ttttaaagtt ttgcccagac ttttactttc ccttgctgta tttgaaaccc
      361 tgacatttgc aatagttgca atattcatac caacaaatgc agcaagctga ctcatagttg
      421 caccaattaa aaaagccatt cctgtatacc atgatacaaa aatcattagt gcacctgcta
      481 caagtatacc gatttttaaa acaactttga actcatgcat aataaatgca tccgcacctt
      541 catgtatggc tccggcaatt tcattcatta attctgtacc ggtattaagt ttttttacag
      601 aaaaatgatt ataggctgca aaccctagtg atgctattgc tgcgattgct acaaatccaa
      661 gcataaaaaa aaacctccct gcacatttta ttttttgtcc agccgtgatt atttccagta
      721 taaggaagta ttctacggtt aagcttaata ttttagaaat atgattggac aagttaaatt
      781 atcaccaatc catcccattt tatcataatg caaaaggttt attcggtcaa aacaaattaa
      841 agcagtattt ctgttttaat aagcataaat actgctttcc tttaaatatc tataaattcc
      901 tccggtatgt tattttttca aggggtatag ctaaagcatg gaacatttca tccatttatt
      961 tcatccaaag caaaacactt tcatttttat agaactgttt atagaactgt agtatttaaa
     1021 attttaacaa tttattgtaa tgaacactcg tgacttaagt catgagataa acaaaaagga
     1081 cagggtgtta ttttagaaca tcctgccttt ttcgtggagg cttgagactt ataattgtgt
     1141 cttaagctta ttaattatat taactttaaa ttactataac tttgtatgtt tttctgcaag
     1201 tcttgaaact attgacttcc atattaatat gcaaatagct taaaaaggct ttaatataat
     1261 atgcaaaact tcattaaatt gcataaaact tttcataaaa tttattgcca tcatataaat
     1321 attgtgtttt tgtgatactt acccgcactt tgaaaatcca cagtttctac atacaacaca
     1381 accgccttca tgctcaagtt tttttccaca atcgggacaa tatacatatt ttagttcatt
     1441 ttcaacatca gtatcttcgt attctgacaa aatttgaagc tcttcttctt tcccatttcc
     1501 attattttga agctgcatta ctttttctat tacccttcct attgcatccg ggcaagacat
     1561 aacctttaag cctttttgcc ttatagttgt tgcacatctt atccccttta gttgttccac
     1621 taatgatttt acatctattc ccgatcttaa cgcaatagaa ataagcctgc tggtagcctc
     1681 tgattgtgaa gggcatccgc caccccttcc aagatttgta aatacttcgc aaattccttg
     1741 ctcatcataa ttcactgtta tataaagatt gccacagcca attctcacct tttcagtaaa
     1801 accggttgta atatccggcc ttgccctggg agcaacatac tttactatta taccttgcgg
     1861 attctccaaa cctttgtccc cttctgtgtc tttctttcct tctgtatttt tcttttcatc
     1921 tttacctttt acgcttccta tattgaggac ttgtgcgtcc ctgcttccat cccggtaaat
     1981 agtaacaccc ttgcaatttg ttttgtaagc tagctcaaac acatccttta catcatcctt
     2041 tccagcctca tttcgtaaat taacagtctt tgatacagca ttatcagtat acttctgaaa
     2101 tgtggcctgc atatttacat gccattccgg tgtaatgtca tgtgacgtaa caaatatatc
     2161 ctggatttca gcaggaatct catcaaaatc ttttagtgtg cttttctttg ctatactttt
     2221 catcaattca tctgaataaa atccattttt aattgctatc tctttaaata caggattgac
     2281 ctcaaccaat tcatcattat ccattacatt tcttatatag catattgcaa aaattggctc
     2341 tattccactg gatacgcctg ctataatgct caatgtacct gtaggagcaa tagttgttgt
     2401 tgtagcattc ctaattttaa taccttgatc tttataaata ctcttatcat aaaaaggaaa
     2461 tactcctttt ctctccgcta tctccataga agctttttta gactcttcct ggataaatcc
     2521 cataactttc tcagcaagat ctatggcttc ttgtgaattg taagatatac ccattttaca
     2581 taacatatca gcccagccca taatcccaag accgattttt cttgtaccct ttgtcatcct
     2641 gtcaatttct tcaagtggat atttgttaac atcaatcaca ttgtccagga aatgcacagc
     2701 ttttttaact acactcccta acttgtcaaa atcgacttca aatccctttt ttaccttctt
     2761 cagcatcagg ctcaagttta atgaaccgag attacatgct tcataaggaa gaagcggttg
     2821 ctccccacaa ttatgagcta ctataccttg tgcaataaga gaatgagtat ctttttcatt
     2881 tatatcaaaa acctctactt cttcaaagtc ttctaccgca aatactcttg acatgaactt
     2941 tattttcttt cttgatattt tagctgcctt tttaagttta gcattttttc ctctatgtat
     3001 aaggtcgcca attgtttttg agaaattata catgtcatct ccgtttataa taagctcgta
     3061 atatatgcct cccgtatatg ttctttcatt tcctgcggta tcaacatatg taaaactttt
     3121 ttggttaatc ttttctcgtt tataaatgga acttgatatt ccaaagttta ataacaataa
     3181 ttgtacatcc ttaagcaaat attccgatgc agatgataaa cgaatatccc tatgattttc
     3241 gtcaacataa tttactgtcc catcagaact gaataatccg tttaaatagg ctttaacagt
     3301 ctcttctgat gaggtaaaga tggctttagg cacacgtttg tcaggagatt ttaccgggag
     3361 caatccgtaa tctttaattt tttctataaa ctccttcttc ttatacataa cttgccacgt
     3421 attgttttgt ctcatgtgtg gttttgggca tccggctcca tatccgcatg caatttgatc
     3481 tatatgctca taaatatagt attcctcgtc agcaaacaca agaccgacta catctccgtc
     3541 cgaggtcatc catccatcac cggttaccct tcctaataat aatcccatct ctctacctat
     3601 attatccttt tcagggaatc tacctttacc cgattgaata agtacataat catcttcact
     3661 aagttgtccg gcctctttcc aaccttgtag agtaagtatt ttatggtcat ttgtaacttt
     3721 taattcctgc ccgttactta aagtaatttt tactgttttc tttacacctg tcttaaatac
     3781 tcttgccttc tttttactaa caccattaat catatactct ctataattcc tagtaatggt
     3841 ctttccacta actctattat ctacaaaaat atcaaattca ctgtcaccat gtttttcata
     3901 aagctctttt atacttatca atccatctgc tgtagatatt aatgtatctg ggtgaaaaca
     3961 cggattagta gactctattt ctcccagctc cggtgtaata ttatccttat ttaatctgtc
     4021 caggaatata atgcctggct caccattatt ccaggcatta tccactatca tatcaaatat
     4081 ttctcttgca ctctcttgcc gtatcggcag ttttgtttta gggtctatta aatcatattt
     4141 ttcattgttc tcaacagctt tcataaattt ttcagttata cccacgctaa tgttaaagtt
     4201 ggttatatcc ttgttatcct gcttacaggt tataaattca cgtatatccg ggtgatctac
     4261 cctgagaata cccatatttg ctcctcttcg tgttcctcct tgctttactg cttcggtagc
     4321 agcattaaag actttcataa aactgacagg gccgcttgca acaccacctg tcgatttaac
     4381 ggaagacccc ttggccctga gccttgaaaa actaaaccct gtacctccgc cgcttttatg
     4441 tattagcgct gcgtttttta tagactcaaa tatgccttcc atggtatctt ctaccggaag
     4501 aacaaaacaa gcgcttaatt gacctaatgg tctcccggca ttcattaatg tgggtgaatt
     4561 cggaagaaat tccagttcgg acatcatctt atagaattca tcttctatag tctttaactg
     4621 tgaagagtcc gcataaccaa aatctgcttc ggcaacagct ttagcaattc ttctgaacat
     4681 accttcagga tcctccaata aattcccatc ttcatctttt gtcagatatc ttctttccag
     4741 aactttaatt gcattttcgt taagattcat aaataagttc cccctgaatt taaaatatta
     4801 ctacatttag tgttaatgtt tttgttaggc acaatatatt gtattattat tttctttata
     4861 tgtcaacgca attatctaaa gttttacact ttttttacga aagcactgaa attaagccat
     4921 ttacaatctt gat
//
LOCUS       DAOSDD010000294         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6085_length_4917_cov_5.524064, whole genome shotgun sequence.
ACCESSION   DAOSDD010000294 DAOSDD010000000
VERSION     DAOSDD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_4917_cov_5.524064"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..742
                     /locus_tag="PK733_17740"
     CDS             68..742
                     /locus_tag="PK733_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOJ12410.1"
                     /translation="MTFKDIFKSSFLEKVVSFSPLDMVIALALAFALGLFIFIVYKKT
                     FKGVMYSASFGLSLLLMTLITTFIILAVTSNVILSLGMVGALSIVRFRSAIKEPIDIA
                     FIFWAISSGIVLGAGLIPLAIFGSIFIGIVLIIFANKKSSDNPYILVVNLIGEDIEKE
                     VMSAIGNKVERYIIKSKTIQKDDNIELSVEVRLRESSTGFVNDICKLNGVDNAVLVSY
                     NGEYMG"
     gene            775..2640
                     /locus_tag="PK733_17745"
     CDS             775..2640
                     /locus_tag="PK733_17745"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOJ12411.1"
                     /translation="MITSKYITQIIAVIMVLAVLFTGAAMALKKSDSVTSTVTQPEYV
                     SRIFDKDAVLDINIEIAEDDWNWLIENAINEEYRPCNITVNGDTYKYVGIRPKGNSSL
                     TQVYRDDTTDRFSFKLSFGKYIKGQTLYGLSTIALNNVMSDATYMKEYISYDLMAEMG
                     VPSPVYTYADIKVNGEPWGLYLAVEIIDRSFLERYFGTGNYNLYKPEPMDMAGGGEFS
                     KMREMQQNLHNNMGNQPNQNNQSNQNNQGNQDNQNKWNNWNAPNTRGSRQLYNNVQNA
                     GNAAGVMRPGNAADGMRRGEPGRMGGMGASGGSNLKYTDDNPESYSVIRNGAVLKTTG
                     DTDFKKVVSMIKALDSGENLEKYLNVEEVLRYFAVNTFLVNLDSYSGGMYHNYYLLED
                     NGIFQILPWDFNLSFAGFGMQDAQRAVNFPIDTPTTDSLENAPLIGKLLEVPEYKELY
                     HKYLQEIVVNYIKSGKFQQTISKLNTLISNYVKNDATAFYTYEQYQASLPVLAQFASD
                     RTASIKAQLSGEQLSDSYGSINTTVNLQTLGGMGGMGDGGAARGPQGAMPGQIPGMNA
                     DQPAMKGNWPGMNEDPNNNNLNNSSREYHLIIMLCLISLAGGIMFAWKFKRKKYN"
     gene            complement(2998..3987)
                     /locus_tag="PK733_17750"
     CDS             complement(2998..3987)
                     /locus_tag="PK733_17750"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOJ12412.1"
                     /translation="MSNKLITCVLAVLCICILTTGCSFMGPQYNFGFIDKTGNFVIDP
                     QYGNALSFTEGLAGIMVGDSQYGKWGFIDMNGNMVIQPQYDKVRGFYEGLCPVMINDR
                     KNSKWTFIDAKGNTVIGTEFTDASIFVKGLAPVKFSDNDLYGYIDPSGNTVIAPKFDI
                     ASIFSEGLALYGVGNVESPETGFIDREGNVVIEAQYAHCGAFSEGLSAVKISRDQEKA
                     DWGFIDSTGKLVIEAKYAWALEFNEGLAPVKIGNTEDGKWGYINKKGELVIENKFGYA
                     FGFSEGLAAVLDGDLKSGKWGFIDTNGNFIIEPRFNSAGYFKQGLAPASVEKK"
     gene            complement(4267..>4917)
                     /locus_tag="PK733_17755"
     CDS             complement(4267..>4917)
                     /locus_tag="PK733_17755"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12413.1"
                     /translation="IFPINSWQAHGPQDRMLNMNIISLQVDNDSLGEIAYSIYGKFNI
                     CFKQGNNTLSNDLKALVELFIEEYRSIQPGRNLILKSLSTQILASLIEQIENNMPSPV
                     SSSYSTAKKNLDKAIEYLRDQYDEDYSLEEAAQVARLSCFHFIRVFKSYTGKTPYEYL
                     MLFKLNKAKELLISNRSSITEICFMCGFNSLSNFTNFFKKRVGVTPSEYRKIMAIK"
BASE COUNT         1522 a          858 c         1012 g         1525 t
ORIGIN      
        1 agtttcaaaa tatgctcttt gcaggagctt tggctaaaga acaatgaaaa cataggggga
       61 ataaatcatg actttcaaag atatttttaa atcaagtttt cttgaaaagg ttgtatcatt
      121 ttcgccactc gatatggtta tagcacttgc tcttgctttt gccctcggcc tttttatatt
      181 tattgtatat aaaaagacct ttaagggtgt tatgtattcg gcaagcttcg gcttatcact
      241 gcttcttatg acacttataa caactttcat catattggct gttacatcaa atgtaatact
      301 gtccctcggt atggtaggtg cattgtcaat tgtacgtttc agaagtgcaa ttaaggaacc
      361 aattgacata gcgtttattt tctgggctat atcctccggt attgtgcttg gtgcaggact
      421 tatacctctt gcaatattcg gctctatatt tattggtatt gtgttgatta tttttgcaaa
      481 caaaaagagt tcagataatc cttatatcct cgtagtgaat ttaattggtg aagatattga
      541 aaaagaggtt atgagtgcca ttggaaataa agttgaaagg tatatcatta aatccaagac
      601 tatacaaaaa gatgataata tagagctttc tgttgaagtc cgtcttaggg aaagttcaac
      661 cggttttgtt aatgacatat gcaagcttaa tggagtggat aatgcagtac ttgtaagcta
      721 taacggggag tacatgggtt gatgcagtag cgggaaggaa agaggtgata tcctttgatt
      781 acaagtaaat acattacaca gattattgct gttataatgg tactggcagt tttatttacc
      841 ggagcggcta tggctttgaa aaagtctgat tcagtcacct caaccgtaac tcagccggaa
      901 tatgttagca gaatatttga taaagatgca gtattggata ttaatataga aattgcagaa
      961 gatgattgga actggcttat tgaaaatgct attaatgagg aatatcgtcc ctgtaatatt
     1021 actgttaacg gagacaccta taagtatgtg ggtatacgcc ctaagggaaa ttcaagcctt
     1081 actcaggttt atcgtgatga tactacagat cgctttagct tcaagttgtc ctttggcaag
     1141 tatataaagg gacaaacttt gtatgggctt tcaactatag cactgaataa tgtaatgagt
     1201 gatgctacct atatgaagga gtatatttca tatgatctta tggcggagat gggagtacct
     1261 tcaccggttt acacatatgc tgacattaaa gtaaacggtg agccgtgggg tttgtatctg
     1321 gcagttgaaa ttatagatag aagtttcctt gaacgctatt ttggtactgg aaattataac
     1381 ctctataagc cggagcctat ggatatggcg ggtggtgggg aattttcaaa aatgcgggaa
     1441 atgcagcaaa accttcacaa caacatgggc aaccaaccga accagaataa tcaaagcaac
     1501 cagaataatc aaggcaatca agacaatcaa aataaatgga ataattggaa tgcgccaaac
     1561 actcgaggtt caaggcagtt atataacaat gtacaaaatg caggaaatgc agcaggtgtt
     1621 atgcgtcccg ggaatgcagc agatggtatg cgccggggtg agccggggcg tatgggcgga
     1681 atgggtgcgt caggtggttc aaacttgaaa tatacagacg ataatccaga aagctattct
     1741 gttattcgga atggtgcggt attaaaaact acaggagata cggattttaa aaaagttgta
     1801 tctatgatta aggcgttgga tagtggtgaa aacctggaaa aatatcttaa tgtggaggag
     1861 gttttacgtt attttgctgt aaatactttc cttgtaaatc tggatagcta ttcaggaggc
     1921 atgtaccaca attattatct gctcgaggat aacggtatat ttcagatact gccctgggat
     1981 tttaacctgt catttgccgg ttttggtatg caggatgcac aaagagcggt gaattttccc
     2041 atagatactc cgacaactga cagccttgaa aacgctccgc ttattgggaa acttttggaa
     2101 gtgccggaat acaaggagct ttatcataaa tatttgcaag aaatcgtcgt aaactatata
     2161 aaaagtggta aatttcaaca gaccatatca aagctcaaca ctcttatcag caattatgtg
     2221 aaaaacgacg caacggcatt ctacacgtat gaacaatatc aggcttctct acctgtgctt
     2281 gctcagtttg caagtgacag gactgcaagt attaaagccc agctttcggg agaacagctt
     2341 tcagattcat atgggagtat taacacaaca gtaaatctgc aaactcttgg tggtatggga
     2401 ggtatgggcg atggcggagc agcaagaggc ccacaaggcg ctatgcccgg acagataccg
     2461 ggaatgaatg ctgatcaacc ggcgatgaaa ggcaactggc ccggaatgaa tgaagatcca
     2521 aacaataata acttaaataa tagtagtcgg gaatatcacc ttataattat gttgtgctta
     2581 atatctttag ctggtggcat tatgtttgca tggaaattta aaaggaaaaa gtataattaa
     2641 atttgtgggg acatttctca gtaaaagagg ttagaagtta gaggttagaa gttagaggtt
     2701 agaagttaga ggttagaagt tagaagttgg aagttggaag ttagaagtta gaagttggaa
     2761 gttggaagtt agaagttgga agttagaagt tagaagttgg aagttagaag tgtgaagttg
     2821 gaagttagaa gtgtgaagtt tgaagcgtga agtatgaggc aagaaataga atttatttaa
     2881 tgtttttttc actaacttct caccaccaac ttccaatctc caactcatca cttcttatct
     2941 ctcacctctc acacctaact tccaaaatga aaaggcatat tcctgcgttt cgagagatta
     3001 tttcttttct actgatgcag gtgcaagtcc ttgcttaaaa tagccggcag agttgaaccg
     3061 gggttctatg ataaaattgc cgttcgtatc tataaatccc catttcccgg acttaagatc
     3121 tccatcaagt actgctgcga gaccttcact aaagccaaat gcatagccaa acttgttttc
     3181 aattactaat tctccctttt tatttatata cccccatttg ccatcctcag tgttcccgat
     3241 ttttacagga gcaagtcctt cattaaattc tagtgcccat gcatatttag cttctattac
     3301 cagcttgccg gtgctatcta tgaatcccca gtcagctttc tcctggtctc tgcttatttt
     3361 taccgcactg agtccttcac tgaaagctcc acagtgggca tactgtgctt caattactac
     3421 attcccttcg cgatctatga atccggtttc agggctttca acatttccaa caccgtaaag
     3481 ggccaatcct tcactgaata tggaagcaat atcgaattta ggagcgataa cagtattccc
     3541 gctggggtct atataaccgt acaaatcgtt atcagaaaat tttacgggag ctaatccttt
     3601 aacaaatatg gatgcatccg taaattctgt accaatcacg gtatttcctt tggcatcaat
     3661 gaatgtccat ttactatttt ttctgtcgtt tatcattaca ggacataatc cttcataaaa
     3721 acctcttacc ttatcatatt gaggttgaat aaccatattt ccattcatat ctatgaagcc
     3781 ccatttgcca tattgggagt cgcccaccat aatgccggcc agcccttcag taaagctcag
     3841 tgcattacca tattgcggat caataacaaa attaccggtt ttatcaataa aaccgaagtt
     3901 atactgtggt cccatgaatg agcatccggt cgtaagtata catatacaca aaactgctaa
     3961 aacacaagta attaatttat tagacattta aaaacctcca ctgctttttt atttacttga
     4021 aaccagtaca atttgatttt gtattgattt ttattatatt acattgtatc atttcttata
     4081 gcaaatgaga aagtattaat tttcacaggt gcatatttta tttttcggag agattggaga
     4141 aattaataaa agaagcatta gctaatatac tatagctggg caggaggaca tttttctctc
     4201 aggtgaaagg atgggatgaa tataaatata tatgagttat gggaatttac gtttgaggac
     4261 tacttgtcat ttaatagcca taattttgcg gtattcagaa ggcgtgaccc caaccctttt
     4321 tttgaagaaa ttagtaaaat tgcttaagct gttaaacccg cacatgaaac agatttcggt
     4381 tatacttgat ctgtttgaaa tcagcagttc ttttgcctta ttcaatttaa aaagcatcag
     4441 atattcataa ggggttttgc cggtataaga cttaaacacc cttataaaat gaaaacaaga
     4501 taaacgtgct acctgagccg cttcttccag ggaataatcc tcatcatatt gatctcttaa
     4561 atattcaata gccttatcaa gattcttttt agcagtgcta tagcttgatg atacaggtga
     4621 aggcatgtta ttttctatct gctctataag acttgcaagt atttgtgtac taaggctttt
     4681 gaggatgaga ttgcgtccgg gctgaatact cctgtattcc tcaataaaca gttccaccag
     4741 tgcttttaga tcatttgaca gggtattgtt accctgttta aagcatatat taaatttacc
     4801 gtaaatcgaa taggcgattt cacccaaaga atcattatct acttgcaggg aaatgatatt
     4861 catattgagc atgcggtctt ggggaccgtg tgcttgccag gagtttatgg gaaatat
//
LOCUS       DAOSDD010000295         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6180_length_4854_cov_5.278391, whole genome shotgun sequence.
ACCESSION   DAOSDD010000295 DAOSDD010000000
VERSION     DAOSDD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6180_length_4854_cov_5.278391"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..909)
                     /gene="pgmB"
                     /locus_tag="PK733_17760"
     CDS             complement(232..909)
                     /gene="pgmB"
                     /locus_tag="PK733_17760"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOJ12414.1"
                     /translation="MMIKLQAVIFDLDGVITDTANIHFAAWKKLTDEINVYFDREINE
                     LLKGVDRMSSLEMILKNSKKQYTKQYTYEEKLSLTDRKNNYYRNLLETITADSILPGA
                     LNTLKLLKNLKIKTGLASASKNAFLIIDKLGISKYFDYIADASKIKKGKPDPEIFLTT
                     ADNLKVRSEECMGVEDSEAGIKAIKAACMYALGIGDSNILKDADYVIKSLEDFNISKL
                     KFYCNRN"
     gene            complement(1056..2123)
                     /locus_tag="PK733_17765"
     CDS             complement(1056..2123)
                     /locus_tag="PK733_17765"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12415.1"
                     /translation="MGSMTSYERFKRMFEHKEADRVPICDVPWSGTIRRWHREGMHEG
                     INYVDYFDLDKVAYIDVDNSPRYEEKIIEETDKYKIFTTKWGVTLKRWNNEDSTPEFL
                     DFKVTDPDRWTEAKKLMVPAKDRINWNYLKKNYKKWREEGYWIQAGLWFGFDVTHSWT
                     AGTERLLIALMEEPEWCVDMFNHYLDVNIALLDMVWDEGYKFDMVKWPDDMGYKYKQF
                     FSVKTYRELLKPVHKRAVDWAHAKGVKTQLHSCGDVNPFIPEFVDIGIDSLNPLEVKA
                     GMNPVEIKKKYGNVLVLHGGINAVLWDDIEEMEAEIKRILQIMKESGGYIFASDHSVP
                     NNVSLEKFKRVVEMVKVLGSY"
     gene            complement(3013..>4854)
                     /locus_tag="PK733_17770"
     CDS             complement(3013..>4854)
                     /locus_tag="PK733_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12416.1"
                     /translation="TDAFKTAFDYANSKDIDVLIPEGNYYVDSQVNRQVFIQTNVECK
                     GKIYTTNKGLTPLFIISRKGEPVIINGSSIAGSLEAGTKRIPELEQYADYDVIFRSNE
                     RLMYRYSGTPEQKGYYTKNEANRINSDGSLKVKTVESYNMKNLLAMKLYKRERPLKLE
                     GLDILCIEKTVTEYGKNIIEVRRSDVTFENLKLDNENLEKEIGQYIGVLISEAVNVTF
                     NNSKIRGFMRQGLGYPVRPQISLYVTLNNTDISYARHAVSGMYDNYTTINGGSYEAYG
                     GVIDTHWGYNIIVNDAVITSNSTPFCINGGSLTVNNCDITTGHYMLINRRMDTPYIKG
                     NVEMKDTKVKYTGTTTFSLYYVQTTTFDSDSELYNANLIIENMKLELVNPIPIVDLYY
                     IYTPMDKNYIMQYLPETIQIKDLYITGINDTKLKSTYFRMMPATSKYYTGNPGVFLEN
                     IKVNRDGNSVNNYDGLIAYYLHPDNTEVKDTHFNVKVKNCGTLSFEIVPSILGDMLIE
                     NCKIVKYSYYGDLSKDWHNNPVWWQKGEGKTIFKNVKFDRTGLSPSAIYIMNDVEFEN
                     CEFTNFGTIDYMNFGKQLPAVKILKVKDCKFNDGGITGGNLSEYMKQ"
BASE COUNT         1443 a          957 c          620 g         1834 t
ORIGIN      
        1 ttctcaacat tcaaggattt catcaaatct gtaatagaaa tcatattaat cacctgccat
       61 gtgttatttg taatattaaa aattcaagcc gatttgtaaa tagtacccat actatgtatt
      121 atctattggc agcatagcct tcaaagcaat atcacccgga tcataaatca ctgtccgtca
      181 cttttcagcc attctcttat ttgggtaaac ataatgtctc ctctttacta ttcagtttct
      241 attgcagtag aatttcagtt tgctaatgtt aaaatcttcc agactcttaa ttacataatc
      301 ggcatctttg agaatattac tatcacctat tcccagtgca tacatgcaag cagcctttat
      361 ggccttaatt cccgcctctg aatcttcaac tcccatgcat tcttcagagc gtactttaag
      421 gttatctgca gtagtcaaaa aaatttccgg atcaggctta ccttttttta ttttacttgc
      481 atcagcaata taatcaaagt atttgcttat tcctaattta tcaattatca aaaaagcatt
      541 tttacttgca gaagcgagtc ctgtttttat ctttaaattt ttcaaaagtt tgagtgtatt
      601 caaagcacct ggcaatatac tatctgctgt tatggtttcg agtaaatttc tataataatt
      661 atttttcctg tctgtcaatg aaagtttttc ttcatatgta tactgttttg tatattgttt
      721 tttagagttc ttcaatatca tttcaagtga tgacattcta tcaacacctt taagtagctc
      781 gtttatttct ctatcaaagt atacatttat ttcgtcggtt agctttttcc aagctgcaaa
      841 atgaatattt gcggtatcgg taataacacc atcaagatca aatattacag cttgcaactt
      901 aatcatcata tttttcacat tctcccctat aaataattta aattaaacca tcataattat
      961 taacactgtt ggcatctcta ttaatcttga tattttccaa ataaatatcc ggattctctt
     1021 tcaggtaata cttgctagtc aaaggattag ttaactcaat agcttcctaa tactttgacc
     1081 atttcaacta cccttttaaa tttttcaagg cttacattat tcggaacaga atgatcggaa
     1141 gcaaaaatat accctcctga ttctttcatt atctgaagaa ttctttttat ttcagcttcc
     1201 atttcctcaa tatcatccca taacactgca ttgatgccac cgtgaagaac aagaacattg
     1261 ccatattttt tctttatttc taccggattc attccagctt ttacttccag tggatttaaa
     1321 gaatctatac ctatatctac aaattctggt ataaacggat ttacatcacc gcatgaatga
     1381 agctgagttt taacaccttt tgcatgagcc caatcaacag ccctcttgtg cacaggtttt
     1441 aagagttctc tgtatgtttt tacagaaaaa aactgtttat atttgtatcc catatcatca
     1501 ggccacttta ccatatcgaa cttgtatcct tcgtcccaaa ccatatcaag tagagcaata
     1561 tttacatcca ggtaatggtt aaacatgtca acacaccatt caggttcttc cataagtgct
     1621 ataagaaggc gttcagtgcc agcagtccat gaatgggtaa catcaaagcc aaaccataat
     1681 cctgcctgaa tccaataacc ttcttcacgc catttcttat agtttttctt tagatagttc
     1741 cagttaatcc tatcttttgc aggaaccata agtttctttg cttcagtcca tctatcagga
     1801 tctgttactt taaagtctaa aaattcgggt gttgaatctt cattattcca tctttttagt
     1861 gtaacacccc attttgtggt aaatatttta tatttatctg tttcctcaat aatcttttct
     1921 tcatatcttg gtgaattatc tacatcaata tatgcaacct tatcaagatc aaagtaatct
     1981 acgtaattaa taccctcatg cataccctcc cgatgccacc gccttatggt accggaccat
     2041 ggaacatcac atattggtac cctatcggct tccttatgct caaacatcct cttaaatctt
     2101 tcatatgatg tcatgcttcc cataatttgc ctcccttgat tatactatcg ctcctgtgta
     2161 taagaacatt cataactact ataatgatgt gctgtgatgt cattttatac ttaatttcat
     2221 gtttttcaat aacaaatatt taagttatta aaaatttata cttgacttat gcctggaaca
     2281 tatccatatc acattagagc atttcactct atataaaaat gaccggatac atatccttta
     2341 ttactttatc aatatcttgt atttgctata ttttatttat gatttcaaaa gtaatattaa
     2401 agtaacatta tcgaggcaat aaaattgatc ttcaagttga acaatatgcc attcgctaaa
     2461 actgctaatt gtaacattta tattcctttc gtttcttgta acctgtcaaa ataataaata
     2521 catacaaata aggcattagt ctcctccgcg ctttgactaa tcaagataca gcttcgccct
     2581 ttcagctgcc ttctttttcc caaaagacac cctagcgaac aaccactata tgatccttat
     2641 cttctatgaa tgcgccccac cacaccattt ccaataagta cttataaatc aacaaaagac
     2701 taaatcatca ttttcttttt ttatatatgt tgcaataaat atattacatt ggaaaaattt
     2761 tagatcattg caacttatga attaaggtat atgatttctt actcgggttc cttctgcatc
     2821 tgcttatgat ttatgtatat aaagtaaaaa taaatatagt tagtttgaac taattgtact
     2881 gttctactat gataatatca agactcattt ttttacattc tgtatcagtt tatgatttat
     2941 gtaagtaaag taaaaataga tacagaatgt aagtattgtt tgctttttaa actacttcct
     3001 gaacctggaa actcactgtt tcatatactc acttaaattg cctcctgtaa tgccaccatc
     3061 attaaatttg cagtctttta ctttaagtat ctttactgca ggcagctgtt taccgaaatt
     3121 catatagtct atggttccga agtttgtaaa ttcacagttt tcaaattcaa catcgttcat
     3181 aatatatatt gcagaaggtg aaagcccggt tctgtcaaat tttacatttt tgaatattgt
     3241 tttaccttca cccttttgcc accatacagg attattgtgc caatcttttg ataagtctcc
     3301 ataataactg tattttacta ttttacaatt ttctatgagc atatctccca atattgaagg
     3361 tacaatttca aatgataatg ttccgcagtt tttcactttc acattgaaat gcgtatcctt
     3421 aacttctgta ttatctggat gtaagtaata tgcaattaag ccatcataat tattaacact
     3481 gttgccatct ctgttaacct tgatattttc caggaataca ccgggatttc ctgtgtaata
     3541 cttacttgtc gcaggcatca ttctaaaata tgtacttttt aatttggtat cgtttattcc
     3601 agttatatat aaatctttta tctgaatggt ttccggcaaa tactgcataa tatagttttt
     3661 atccattgga gtgtatatgt aatataaatc tactataggt ataggattta ccaattccaa
     3721 tttcatgttt tcaattatta agttggcatt gtataattca gaatcactgt caaaagttgt
     3781 agtctgtaca taatataagc taaaggttgt agtacctgta tatttcactt ttgtatcctt
     3841 catctctaca ttacctttta tgtaaggtgt atccatcctt ctgtttatca gcatgtagtg
     3901 tcctgtagtt atatcacaat tatttactgt cagacttccc ccattaatgc aaaagggtgt
     3961 actgttgctg gttataacag catcgtttac tataatatta taaccccaat gagtgtcaat
     4021 tactcctcca tacgcttcat agcttcctcc gttaattgtt gtgtagttgt catacattcc
     4081 tgaaactgca tggcgtgcat aactgatatc agtattattt aaggttacat aaagagaaat
     4141 ttgaggacgt accggataac caaggccctg acgcataaag cctcttattt ttgaattgtt
     4201 aaaagtaaca tttaccgcct cagatattag tacacctata tattggccaa tttctttttc
     4261 caggttttca ttatccagct ttaaattttc aaaagtcaca tcacttcttc taacctcaat
     4321 aatatttttg ccatactctg tgacagtttt ctcaatgcaa agtatatcaa gtccttctaa
     4381 tttcaatggt ctttctcttt tatacaattt catagctaat aaatttttca tgttataact
     4441 ttcaactgtt tttaccttaa gtgaaccatc gctatttatc ctgttagctt cgttttttgt
     4501 ataatatcct ttttgttctg gagttccgct atacctatac atgagtctct catttgatct
     4561 gaatattaca tcataatctg catattgttc cagttcgggt attctctttg tacctgcctc
     4621 aaggcttcct gcaatgctgc ttccattaat tattaccggt tctcctttac gtgagataat
     4681 aaacagagga gttaatcctt tatttgtagt atatatcttc cctttacact ctacatttgt
     4741 ttgaataaat acctgtctat ttacctggct gtccacataa taattacctt caggtattaa
     4801 tacatcaatg tcctttgagt ttgcataatc aaaagctgtt ttaaaagcat cagt
//
LOCUS       DAOSDD010000296         4834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6217_length_4834_cov_4.984516, whole genome shotgun sequence.
ACCESSION   DAOSDD010000296 DAOSDD010000000
VERSION     DAOSDD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6217_length_4834_cov_4.984516"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1238..2884
                     /locus_tag="PK733_17775"
     CDS             1238..2884
                     /locus_tag="PK733_17775"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12417.1"
                     /translation="MKGLKKTFYIFVTVIIIIGLCLLSSCNINKAGTIKEETSQGLGV
                     DAKDGILPITFTAFIDRPVSAWNGWGNDPVTKEVTRRTGVSVTVSSATTTDSTKLSAM
                     IATDNLPDFIITRGKSPIQNVLSEQGYLLALNQLMDQYAPEMRKIVPKDLIGLYSNSE
                     GNFYFYPDRFYDVDRVLSVEGYAGAGSTNIRINLTLYEKIGKPPMATLDEYKEALKLI
                     KTLLPDVQFPIYDGNIVMPHHNQNLAQFLNRIYGGTDTKSIANDDTVHLNFKDESYLK
                     AIQYINNLYREGLFNKENFTIQSEQFKEVINNDKLFTHWGPGWPIDSVRGTTDNPYWP
                     IDPPQVPGIQLKIKSQAYSIGGGVCTSISKSCIDPARAIKYLEFMISDEGQLLFGWGI
                     ENKDYTYEENYPRYTQSASDLMSADWDKYNREVGAYNYNSAWILNGLTDVWASYWYAK
                     TADKFIMEPGRIMKKYAVNERLNDLTVLPLGAVDEILIETQIFELWKNSLPSMYLAET
                     EAQCMTAYNDFINKAEKLGLNKLEQAYTEAYRNVKEKLSK"
     gene            3075..3995
                     /locus_tag="PK733_17780"
     CDS             3075..3995
                     /locus_tag="PK733_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12418.1"
                     /translation="MQKVNIIIKKLKMQKYLHIMTIPTVIWLIIFCYIPMYGLQIAFK
                     NFMFNKGILGSAWVGLKHFITFFTDPYIKNVLINTFALSVIRSILIFPIPIIFALLLN
                     EVSNSLFKKITQTISYLPYFISWVMVAIMANSWLSTTGFINKLMMFLGIIEEPYLFLG
                     TPNAFWWIAMFLEIWKTTGWSSIIFLAAISGIDQEMYESSFIDGANRFQRIWYITLPS
                     ILGTIMILLILTLSRVLTSANFEVSYLLSNPLNFSKSDILATYILRIGISLGRYSYAT
                     AVGLLQSVVAFVLLFAANFGSKKATDISFF"
     gene            4093..>4834
                     /locus_tag="PK733_17785"
     CDS             4093..>4834
                     /locus_tag="PK733_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12419.1"
                     /translation="MIAGNNKSLLEKTFESLNYLFLSLFIFFTLYPFYYCVMLSFNDG
                     KDAESGGIYFWPRLFTLENYRRVFSDPYILVGFRNSILRTVIGVVLAVFFTAMYAYAV
                     SRRDLLFKKVYLTIGIITMYFGGGLIPYYILINRIGLIDNFLVYIIPGLFSMSNAILF
                     ITYFRDIPVSLEESAKIDGANDIYIFYKIIFPVSLPIFACIALFIGVSQWNSWFDTML
                     YTNSDNLETLPHLMVKILNTQNYMNEMAA"
BASE COUNT         1658 a          703 c          903 g         1570 t
ORIGIN      
        1 tattatatca atgaatttta agaggtttta ccaccgcgca gcggtttagt ccaataataa
       61 ttatatagta atcgaccgct atttaaacac tactatgagt tatgcaacta tctaaaagag
      121 aagttctcca accaaaatgt gcatttttcc ggcatgggtg cccagggtcc gttaacttct
      181 gctgtactta tgcggggaca ggattttatt tatgacgtgt atgacgagcc ggaaaaggca
      241 aagagatttc ttgctctcct aacagacagc attgttggtt ttcaccattt tactaacaag
      301 gtacttcaaa ttctccaaac taaaaattat acactaaaat caaaaaatta tatgatgaaa
      361 ctaatcatag taaaatgtat actaataata gtcaatagta tatattgatg caaataatta
      421 tttgaactgt caatggccat atgctttata gaattattta tgataattaa atagttatca
      481 cttttgtgca atattgctta cttatgtgat ataaacataa gaatgatctc ggaaagtaat
      541 tgttaattcc aaaatgtgat acatatttta cagcaacaac ctcttaaccg atagtttcga
      601 gtgaattcaa acattgcacc ttgaaaatta aagagttgtg ttataggaga atcaaaaatt
      661 gggcgtatca aagcaaactt atgtcccggg acacaggttt gtgggaaatc aatgtgattt
      721 gaggatagag ttttctaacc tcattaaaag caaagacagt taagatgcta tcatgccttg
      781 gagcttaagt gattcttaat tatatgagca aatcataagg actatcggcg gcagtagtgc
      841 taaatagagt atcttcatag aaatactgct catggtagtt atcccatctc taatgagcac
      901 ctgggtctga gtagcgattg aataaaataa aaagtagcag gagtatcttt tgatagtctt
      961 catgtggttc ccaataacat aacataaatg aaatccctat aataaatgta atggtaaata
     1021 ataacatcca tttctattat gggggttgtt ttggaaaagt caaggtagaa tacaactata
     1081 tatgtactta tatggaaatt caagctagag aatttgaagt aaatatatag agaatattgg
     1141 aggtatgaga aaatactcaa atctcgaaat atagatatcg aaagtgttta tagttttcga
     1201 gagtatcaat atgaatgtga ggaggtatga aaacaatatg aagggtttga aaaagacttt
     1261 ttatatcttt gttacagtta tcataataat tggtttgtgt ttattatcct cttgcaatat
     1321 caacaaagca ggtaccatta aagaggaaac atctcaaggc ttgggggtgg atgcaaaaga
     1381 tggaatatta ccaattactt ttactgcatt tattgatagg cctgtttcag cgtggaatgg
     1441 atggggtaat gaccctgtaa ctaaggaagt aacaaggaga accggagttt ctgttacagt
     1501 tagttctgca acaacaactg acagcactaa gttgagtgcg atgattgcta cagataacct
     1561 gccggatttt ataatcacac ggggcaagtc tcccatacag aatgtactct cagagcaagg
     1621 atatttactt gcgcttaatc aactgatgga tcaatatgca ccagaaatga ggaaaatcgt
     1681 tccaaaagat ttgattgggt tatactcaaa ttctgaaggt aatttctatt tttatccgga
     1741 tagattctat gatgtagata gagtcttgtc agtagaagga tacgccggag ccggatctac
     1801 aaacattaga ataaatttga ctttgtatga aaagatcgga aaacccccta tggcaacatt
     1861 ggatgagtat aaggaagcgt taaagctgat taaaacctta ttacccgatg tgcaattccc
     1921 tatatatgac ggtaatatag tgatgcctca ccataatcaa aacttggcac aatttcttaa
     1981 caggatatat ggaggtactg acacaaaatc aatagctaat gacgatacag ttcatttaaa
     2041 ctttaaggac gaatcgtatc taaaagcgat tcaatatata aacaaccttt acagagaagg
     2101 tttatttaat aaagaaaact ttacaattca gtctgaacaa tttaaagaag taataaacaa
     2161 tgataagctt tttactcatt ggggtcccgg atggccaata gattcggtcc gcggtacaac
     2221 agataatcct tattggccta tagatcctcc gcaggtgcca ggtatacaat tgaaaataaa
     2281 gagccaggca tattctatag gaggtggggt ttgtacgagt atcagtaagt catgtataga
     2341 ccctgcacgg gcaataaaat accttgaatt catgatttcc gatgaaggtc agctgctttt
     2401 tggatggggt attgaaaata aggattatac atacgaagag aattatccca gatatacaca
     2461 atctgcatcg gatcttatgt ctgcagattg ggataagtac aacagggaag tgggtgcata
     2521 caattataac tctgcatgga tactaaacgg acttactgat gtttgggcca gctattggta
     2581 tgccaagaca gctgataaat ttatcatgga gcccggaagg attatgaaaa aatatgctgt
     2641 aaatgaacgg ttaaatgatt taactgtgtt acctcttggc gctgtcgatg aaatacttat
     2701 tgagactcaa atattcgagt tatggaagaa ctctctgccg tctatgtatc ttgctgaaac
     2761 cgaagcccag tgtatgacag catataatga tttcattaat aaagctgaaa aactgggcct
     2821 caataaactg gaacaggctt ataccgaggc ttacaggaat gtaaaagaaa aattaagtaa
     2881 atagatcaaa ttaattacta cgagttttaa ggaagatatc ataacttggg gatatgtgat
     2941 tagctggaca taaaagagaa tttcgctcaa taatacatat cccctgaaat ttatatcata
     3001 gtattataag ttttataatt ataagtgaaa cataatattt aagcagaacc tattatttaa
     3061 aaggtagtga aaaaatgcaa aaagtgaata taattattaa aaaactaaaa atgcagaaat
     3121 atctacatat aatgaccata cctactgtta tatggcttat aatattttgc tatattccta
     3181 tgtatgggtt acagattgca tttaaaaatt tcatgttcaa taaaggcata ttgggaagtg
     3241 catgggtagg tctgaaacat tttataacat tttttaccga tccttatatt aaaaatgttt
     3301 tgataaatac tttcgcgtta agtgttataa gatcaatctt aatttttcca attcctatta
     3361 tattcgcctt acttttgaac gaagtctcta atagtttatt taaaaagatc actcagacca
     3421 taagttacct gccatatttt atttcctggg taatggttgc aattatggcc aatagctggc
     3481 tatctacgac agggttcata aataagctca tgatgtttct gggaattatt gaggaacctt
     3541 atttgttttt aggtactcca aatgcatttt ggtggattgc tatgttcctc gaaatatgga
     3601 aaaccacagg ctggagttcg ataatttttc ttgcagctat atctggtata gatcaagaga
     3661 tgtatgaaag ttcttttata gacggagcaa acagatttca aaggatatgg tatattactt
     3721 tgccttcaat acttgggaca ataatgatac ttttaatatt aacactcagc cgtgtactaa
     3781 cgagtgcgaa ttttgaagtt agttatcttc tgagtaatcc attgaatttt tcaaagtcag
     3841 atatactagc cacatatatt ttaaggatcg gtatatctct gggaagatat tcatatgcaa
     3901 ctgctgtagg tttgttgcag agcgttgtag cattcgtatt gctttttgca gccaactttg
     3961 gaagtaaaaa ggctacagat attagtttct tttaaattaa atagaattag aaaaataaaa
     4021 aaggattgta catataagct atattatata tagtatttag aaacagttta tgattatgaa
     4081 aggcggatcg aaatgatagc tggaaataat aagtcattat tagaaaaaac ttttgaaagt
     4141 ctcaattatt tatttttatc attatttata tttttcacac tgtatccttt ttattattgt
     4201 gttatgttat cctttaatga tgggaaggat gcagaaagcg gcgggatcta tttttggccc
     4261 aggttattta ctctagaaaa ctatagaaga gttttttcag atccgtatat tttggttgga
     4321 ttcagaaatt cgatattaag aacagtcatt ggtgtagtac ttgccgtatt ttttacagct
     4381 atgtatgcat atgctgttag cagaagagat ttattgttta aaaaagtata tttaactatt
     4441 ggtattataa caatgtactt tggtggaggg ctaataccat attacattct gattaacaga
     4501 ataggattaa tagataattt cttagtatac attattccgg gtttgttttc tatgagcaat
     4561 gcaatattgt ttattacgta ttttagagat attccagtat cactggaaga atcggcaaaa
     4621 attgatggag ccaatgatat atatatattc tataaaataa ttttccctgt atctttacca
     4681 attttcgcat gtatagcttt gtttataggt gtgagtcagt ggaattcctg gtttgatact
     4741 atgctttata caaacagtga caacctggaa acattacccc atttgatggt caaaatttta
     4801 aatactcaga attatatgaa cgaaatggca gcta
//
LOCUS       DAOSDD010000297         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6231_length_4825_cov_3.106499, whole genome shotgun sequence.
ACCESSION   DAOSDD010000297 DAOSDD010000000
VERSION     DAOSDD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6231_length_4825_cov_3.106499"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(703..1518)
                     /locus_tag="PK733_17790"
     CDS             complement(703..1518)
                     /locus_tag="PK733_17790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ12420.1"
                     /translation="MDDVIVRSRYSRNNPTYRKRKSRVKENNRLGEIIKIQILVSILI
                     FLIIWGISKIDATITQFITTNAKWVLSSDMDINNIYNQINSMFNIDNGEIEKEKENEK
                     GNEKESEDSEKTITDSAYIENTTGNNKSLELEFIVPVEGVVSSFFGERLDPVTNKLKY
                     HSGIDIETGIDTQIKAVEAGEAIEISEHRMYGRYIKLKHSGGITTVYAHCSKILIKEG
                     TKVSKGDIIAEVGSTGVSTGSHLHFEIWKDERPQNPLEFINVPIITTNSNEIL"
     gene            complement(1690..2088)
                     /gene="mgsA"
                     /locus_tag="PK733_17795"
     CDS             complement(1690..2088)
                     /gene="mgsA"
                     /locus_tag="PK733_17795"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOJ12421.1"
                     /translation="MNIALIAHDKKKELMADFCIAYKSILENHTLYATGATGTTVTEA
                     TGLSVYKFFPGPLGGAQQIGARIACNEMDLVIFFRDPLTDQEYEPDVNSIIRLCDIYN
                     IPVSTNIATAEMLIKGLERGDLGWRELVNR"
     gene            complement(2108..2389)
                     /gene="minE"
                     /locus_tag="PK733_17800"
     CDS             complement(2108..2389)
                     /gene="minE"
                     /locus_tag="PK733_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOJ12422.1"
                     /translation="MFFDLFKFFSRPRASKEVAKERLKLVLIHDRANVSPQFLEMIKS
                     EIIKVITNYIDVDEESLDIQLTRTKSDDGESIVPALVANIPIKSLKNRK"
     gene            complement(2404..3204)
                     /gene="minD"
                     /locus_tag="PK733_17805"
     CDS             complement(2404..3204)
                     /gene="minD"
                     /locus_tag="PK733_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOJ12423.1"
                     /translation="MGEVIVITSGKGGVGKTTTTANVGTALALNNKKVVLVDADIGLR
                     NLDLVMGLENRIVFDLVDVVEGRCKLRNALVKDKRYEGLFLLPAAQTRDKSSVKPEQM
                     LKLCEELRKEFDYSIIDCPAGIEQGFNNAVVGADKAIVITTPEVSAVRDADRVIGLLE
                     SKELRDPRLLINRIKTDMVRRGEMMSIEDVKDILAVNLIGIVPDDEKIVVSTNRGEPA
                     VTDQKSLAGQAYRNIAQRIDGEEVPLLNLEDDEGFMFRLKRFLGIKST"
     gene            complement(3285..3977)
                     /gene="minC"
                     /locus_tag="PK733_17810"
     CDS             complement(3285..3977)
                     /gene="minC"
                     /locus_tag="PK733_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOJ12424.1"
                     /translation="MDENNSIVFKATNNGLILLMKEDEDFEKIYDQVEKKLASAGRFF
                     KGASLVVKYRGKTLSPDEAERISQLMSDITEAEILAFDEDTNHDPNVLENSFDIEEND
                     QPVSYIKEYYFKGIEEGVTKFYRGTVRSGQLLNFNGNVVILGDVNPGAEVQATGNIIV
                     MGTLRGTVHAGMDGNREAIVAALNLQPVQLRIASIITRPPDDTGVKGPMVPEIAYVKD
                     DMIYIERFLPQR"
     gene            complement(4058..4573)
                     /gene="mreD"
                     /locus_tag="PK733_17815"
     CDS             complement(4058..4573)
                     /gene="mreD"
                     /locus_tag="PK733_17815"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOJ12425.1"
                     /translation="MRAKVFICIVGIFIIILLQTTLLDYIRINDIKPNLILVYIVCSS
                     ILEGSAGGAAIGLFAGLVHDIVSGKIMGFYSILGMYLGAITGLSSRRLYKDNVIVVVL
                     FTFALTIVFESGVYLLSGFGSLRPRLLFALKRIVFPEALYNSAFSVLIHYIVAKVNRR
                     FDFEGKATRRY"
     gene            complement(4573..>4825)
                     /locus_tag="PK733_17820"
     CDS             complement(4573..>4825)
                     /locus_tag="PK733_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ12426.1"
                     /translation="GLCHMYNIPINIDVSFNDVIETSGLGGIYPKGILVGRVKEIRHS
                     DSELDRYGVIEPEVDLKKLQEVFVLKRKEIKEQSGKGVK"
BASE COUNT         1466 a         1017 c          634 g         1708 t
ORIGIN      
        1 ttaaattttt catccaatac atagataata tgaaacctgt caaaatccat gttttttaat
       61 atattcccaa gagacattgt ttttataacc accaactccc ttacgggata aatgcctttt
      121 ttcaataacc ttgaacgtct gaagagtatt tgtttcatat tcaataaagc tccctccata
      181 tcttttgata ttattaaaat aattatatat atacctataa ttattaaact tatattaaaa
      241 aaagaagaaa aaagtatttg tactaaacca aagagtataa aaattatgga aaaggtcaaa
      301 gatatccttt tcatgtatct acctgcaata atgagacctt tttttgaagc cagtatgttg
      361 actaggattc tgcttccatc tagaggtata actggtagga tattaaatat ggcaagatat
      421 aagtttgttt ttgcgaaatc agtaatattt tctttataaa ttaatatacc ggttatgtac
      481 ttgcataata attgtcctga tataaatagt aatgcatttg ttataggacc gatcaaataa
      541 ataaaaattt ttttccatag gcggtctgta ctttcttcga taatagcatt aaaaccaact
      601 ggtaagatcc ttataccatt cactttaatt cctaacacta atgcagcaat tacatggact
      661 gtctcatgca gtaaaattga tataaatgca tacaggaaac tttcaaagta tttcattcga
      721 atttgttgtt attataggta catttataaa ttccagagga ttttggggtc tttcgtcctt
      781 ccatatttca aagtgaagat gagaacctgt agaaacacct gtacttccaa cctccgctat
      841 aatatcccct ttgctcacct tcgttccctc ttttatcaag attttagaac aatgagcata
      901 tacggttgtt atcccccctg aatgttttaa ctttatgtac cttccataca tacggtgttc
      961 agatatttca attgcctctc cggcttctac tgcttttatt tgtgtatcta tacctgtttc
     1021 aatatctata ccactgtgat atttaagttt atttgtgaca ggatccagtc tttcaccaaa
     1081 aaaagatgaa actacgcctt ctacaggtac aataaattca agctcaaggc tcttattatt
     1141 tccagttgta ttttctatat atgcactatc tgtaatggtt ttttctgaat cttcactttc
     1201 cttttcattt cctttttcat tctctttttc tttttctatt tccccattat ctatgttaaa
     1261 catactatta atttgattat aaatattatt aatatccata tccgatgata atacccattt
     1321 ggcattagta gtaataaatt gtgtaatagt ggcatcaatc ttgcttatac cccaaataat
     1381 aagaaatatt agtatcgata caagaatttg tattttgatt atttcaccta atctgttgtt
     1441 ttcttttacc cgtgactttc ttttcctgta agttggatta ttacgggaat atctggacct
     1501 tacaataaca tcatccatat gccgcccaca ccctttttta cataaattaa ttttggggag
     1561 caatcatatt attaaaacat attgctcaaa atgcattgtt catataaatt atattgtaca
     1621 agtccaggat ttattactac ttatatttat ggcgaggaac aaatagaaaa tgtgcttgcg
     1681 cattcatatt tacctgttta ccagttctct ccacccaaga tctcctcttt ccaaaccctt
     1741 gattaacatt tctgcagtgg caatgtttgt agacaccggt atattataga tgtcgcaaag
     1801 acgaattata gaatttacat caggttcata ttcctgatca gttaatgggt ctctgaaaaa
     1861 tataaccaaa tccatttcat tgcaagctat tcttgcaccg atttgttgcg ctccccccag
     1921 ggggccgggg aaaaacttat atacgcttaa tcccgttgct tccgtaacag ttgtccctgt
     1981 tgcaccagtt gcatataaag tatggttttc aagtatggac ttatatgcta tgcaaaaatc
     2041 tgccattaat tctttttttt tatcatgtgc tataagtgca atattcataa taatcagaca
     2101 aatcctgtta ttttctattt ttcaaacttt taattggaat attggccact aatgccggca
     2161 caatactttc tccatcatca ctctttgtgc gtgtcagttg tatatcaagt gattcctcat
     2221 ctacatcaat ataattggtt ataaccttta taatttcact ctttatcatt tccaaaaact
     2281 gaggagatac attggctctg tcatgaatca gtaccagttt cagcctttcc ttagctactt
     2341 ccttcgatgc ccttggtctt gaaaaaaact taaataaatc gaaaaacata ctccttctcc
     2401 ttttcaggtt gatttaattc ccagaaatct ctttagtcta aacataaatc cttcatcatc
     2461 ttcaagattt aatagtggta cttcctcacc atcgattctt tgtgcaatat ttctgtaagc
     2521 ttgtccggct aatgactttt gatcagtaac agcaggttct cccctgttgg tagatacaac
     2581 gattttttca tcgtcgggta ctattccaat caggttaaca gccaatatat ccttgacatc
     2641 ctcaatactc atcatttcac cgcgccttac catatcagtt tttatcctgt ttatgagaag
     2701 tctagggtct cttaattctt tggattccag gagtcctata actctatctg cgtctcttac
     2761 ggcagatact tcaggagttg taataacaat tgccttatca gcgccaacaa cagcattatt
     2821 aaaaccctgc tcaattccgg cagggcaatc aattatggaa taatcaaact cttttctcaa
     2881 ttcttcacaa agtttcaaca tttgttcagg ctttaccgat gacttatccc ttgtttgagc
     2941 tgcaggtaaa agaaacagtc cttcataacg tttatccttt accagggcat tccttaattt
     3001 acatctgcct tctacaacat ctacaagatc aaacacaatc ctattttcca gtcccattac
     3061 aagatccaga tttcttaacc ctatatcagc atctacaagc acaacttttt tattatttaa
     3121 tgcaagagct gttcccacat ttgcagtagt cgtagtttta cctactccgc ccttccctga
     3181 agtaattaca ataacctcac ccattaaatt aaaccccctg tattatgtta catctaaaaa
     3241 aactatatat ctatttatgt aatttgtcaa tcttgatatc tagtttacct ttgaggcaaa
     3301 aatctctcaa tataaatcat atcatcttta acatatgcaa tttctggaac cataggcccc
     3361 ttgacccctg tatcatccgg aggcctggta ataatacttg ctattctcag ttgtacaggc
     3421 tgcaaattta gagctgctac aatggcttct ctattgccat ccatacctgc atggacggtt
     3481 cctctaagag tacccattac aataatattc cctgtagctt gcacctctgc cccagggtta
     3541 acatccccaa ggatgactac attaccattg aagttcaata attgtcccga tcttactgtt
     3601 cctctgtaaa attttgtaac cccctcttct atacctttaa aataatattc ttttatgtaa
     3661 gataccggtt ggtcattttc ttcgatatcg aaactatttt ccaatacatt tgggtcatgg
     3721 ttggtatctt catcaaacgc taatatttct gcctctgtta tatcagacat caattgggaa
     3781 attctttctg cttcatcagg agacaatgtt ttaccacgat actttacaac aagggacgca
     3841 cctttaaaaa acctgccggc agaagcaagt tttttctcca cttggtcata tattttctcg
     3901 aaatcctcat cttctttcat taaaaggatt aaaccattat ttgttgcttt aaaaactata
     3961 ctgttattct catccataaa caatacctcc atgaaaatta agtagtaaat actaatacct
     4021 ggtcagtatt ctgtacttaa tatactataa tacttactta gtatcttctt gttgctttac
     4081 cttcaaaatc aaatcttctg ttgacttttg ctacaatata atgtattaat accgaaaaag
     4141 cgctattata taaggcttcc gggaaaacta tccttttaag agcaaaaagc aaccttggtc
     4201 tcaagctgcc aaatccactc aataaataaa ctccgctttc aaagactatt gtcaaagcaa
     4261 aagtaaacaa aactacaaca ataacattat ccttatatag ccttctactt gacaggccgg
     4321 taattgctcc cagatacatg cctaatatgg aataaaatcc cataatctta cctgaaacaa
     4381 tatcatggac caatccagca aataaaccta tcgctgcccc ccctgcactt ccttccagta
     4441 ttgatgaaca gacaatatac acaagaatca ggttgggttt aatatcatta attctaatat
     4501 aatccaaaag ggttgtctgt aaaagtatta tgataaaaat acctactatg caaataaaaa
     4561 ctttcgccct cattatttta cccctttgcc cgactgttct ttaatttctt tccttttcaa
     4621 aacaaatact tcctggagct tttttaaatc tacctctggt tctataaccc catatctgtc
     4681 caattcactg tcagaatgcc tgatttcttt cactctccct acaagtatgc ctttaggata
     4741 tatcccccct aggcctgatg tttcaattac atcattaaaa gacacatcta tgttgattgg
     4801 gatgttatac atatggcaaa gtcct
//
LOCUS       DAOSDD010000298         4820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6239_length_4820_cov_3.192865, whole genome shotgun sequence.
ACCESSION   DAOSDD010000298 DAOSDD010000000
VERSION     DAOSDD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6239_length_4820_cov_3.192865"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PK733_17825"
     CDS             complement(<1..984)
                     /locus_tag="PK733_17825"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ12427.1"
                     /translation="MAHVVVIGGGLAGCLVAKKLADRGAEVAILEKSENIGGKVKNYG
                     CKATDKCNNCGLCLVGDLWENIDSNSKIEILTNAKLIDIYGSKGNYSVMVKVGNSTEI
                     LNEIESIVVSIGFNNFSLLSSSCLEFDTSNNIITGSDLEKVISCRGTSELFGELYAKN
                     PESIAFIQCFGSRDIKEKAMYCSRICCAYSTRAAKVIKQYYPETIISFFYMDLQWVEN
                     KEYFNSMKNENIEFIECRPVKINSGNPAKIIYEDPKTCKLIERDYELIVLSEGIHPAN
                     DVEHIAEICMLEQDENGFLKYVKSPETTGVYIAGCAGGPKRIEEVYAQSLAI"
     gene            complement(1169..2050)
                     /locus_tag="PK733_17830"
     CDS             complement(1169..2050)
                     /locus_tag="PK733_17830"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12428.1"
                     /translation="MLDAMEAQSYYRIWESTGCNEEINDRYLVVNCTGCCVLPHPFTT
                     HNKSGRKDYYLLYMCKGMMSVLVNGEMKDICAGQFIIFFPGCEYKYIGQGDEGVIYYW
                     VHFTGYGADKLLENCKLYGNNIFSAGFNKQVIHVFNNMFTEFILRDYCFEIAAASHLI
                     SICINLGRSVSNLVTGRDIANRDRIQASIKFIHKNFNSNISLKQLAAIEHLSISRYRT
                     IFKQCTGFSPKNYIIALRVKRACELIMQTDLSLKEIAESVGYQDQLYFSRVFKACTGI
                     MASRYKKSYPNNVNKIN"
     gene            2485..3318
                     /locus_tag="PK733_17835"
     CDS             2485..3318
                     /locus_tag="PK733_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ12429.1"
                     /translation="MKKAINAWSVPNHITFAETFRDISCAGFDGIELNLDADNYSSHS
                     LTMGIGKDTFNEINSLSEEYNLKVVSISTSLTGGKLGSDNKSVRESGKDLIRKQLECA
                     AALGADSILTVPGGMTDSISLLKAYENSFMAIEELKPEIESAKIYVGLENVWNGFFTS
                     PFDMKNFIDKLNCTYIGAYFDVGNVIAFSNPEYWIEILNTRICKIHVKDFMRNNGINR
                     GGCFVNLLEGDANWNKVITELCKIGYNGFLTAELGMMMECPEYLYKITSKALDIIINV
                     R"
     gene            3793..4767
                     /locus_tag="PK733_17840"
     CDS             3793..4767
                     /locus_tag="PK733_17840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12430.1"
                     /translation="MKKLAMIGCGGIGKYHLNHLLSFSDIELAGFCDPILEQAEKFVS
                     EAGSGRAFTNYKEMYDKINPDMVFICIPPAEHGEVEFETINRRIPFFVEKPIALSTDL
                     AKQICYEVKKAELITAVGFQCRYSNLVEPNVRFIKEKEIIFVDCIRIGSVPRVAWWKK
                     RSASGGQIVEQTIHQLDIIRYVLDEPNEVFTMGTKGFITDVEDYDTEDLSTTVVRFKS
                     GALATISTGCYATSSNAADSKIVFSSRDSRAELRILDDLKIFGDDEIAYKEKGDAGIL
                     CDRAFIDSVLTSDGSKIRSSYEDAVCTLEFALACNRSMDTGLPVEIGS"
BASE COUNT         1558 a          821 c          819 g         1622 t
ORIGIN      
        1 aatagccaat gattgagcat aaacttcttc tatccttttt ggtcctccgg cacaacctgc
       61 aatatatacg cctgtagttt cagggctttt tacatatttt aaaaatccat tctcatcctg
      121 ttccagcatg catatctcgg caatatgctc tacatcattt gcaggatgta ttccttctga
      181 aagaacaatc aattcataat ccctttctat tagtttacag gtctttggat cttcatagat
      241 tatttttgca ggattgccac tattaatctt tacaggtcta cattctataa actcaatgtt
      301 ttcatttttc atagaattaa aatattcttt gttttccacc cattgcaaat ccatataaaa
      361 gaatgatatt atagtttccg ggtaatattg ttttattacc ttggctgctc tggttgaata
      421 ggcgcaacaa attctagaac agtacatagc tttttctttt atatctctcg aaccgaagca
      481 ttggataaaa gctatacttt ctggattttt agcatataac tctccaaata attcacttgt
      541 acctctacat gaaataactt tttccagatc actgcctgtt attatgttat tgctggtatc
      601 aaattccaga catgatgaag ataataaaga aaaattgtta aaaccaatag aaacaactat
      661 agattctatc tcattcagga tttctgtact atttcccact ttcaccataa ctgaataatt
      721 acctttgctg ccatatatat caattaattt agcattggtt aatatctcaa ttttactatt
      781 actatctatg ttctcccata aatcccctac aagacacagc ccacaattat tgcatttatc
      841 agtagcttta caaccatagt ttttaacttt accaccaata ttttctgatt tctctaaaat
      901 tgctacttca gcacccctat ctgcaagttt ttttgcaaca aggcatcctg caagtcctcc
      961 gcctatcact actacatggg ccattttcta tcacctattc taataattgt cacataattt
     1021 attgtttttt agcacattaa ttattaattt atataatatt actttttgtt tcatcagaaa
     1081 gtcatttcat gactttctgg acgataacca tattatttca taaactgaaa aggaaagtat
     1141 agaaaatgtt tgcttttttt tgtacttttt agttaatttt gttcacatta ttagggtaac
     1201 ttttcttata gcggctagcc attatgccgg tacaagcttt gaaaacacgg ctgaaatata
     1261 gctgatcctg ataacctacc gattcagcaa tttcttttaa agataaatca gtttgcataa
     1321 ttaactcaca cgctctcttt actcgaagag caataatata atttttgggt gaaaaacctg
     1381 tacactgttt aaaaattgtc cggtaacggc ttatgctcag atgttcaatt gctgcaagct
     1441 gctttagcga tatgttagag ttgaagtttt tatgtataaa cttaattgat gcttgtattc
     1501 tgtctcgatt ggctatatca cgaccggtta ccaaattgga gacacttctg cctaaattta
     1561 tacatatcga aataagatgc gatgcagcag ctatctcgaa acaataatcc cggagtataa
     1621 attctgtaaa catattatta aatacatgta ttacttgttt attaaaaccg gcactaaaaa
     1681 tattatttcc atacaacttg cagttttcca gcagtttgtc tgcaccataa ccggtaaagt
     1741 ggacccaata gtagattact ccttcatctc cctgacctat atatttatat tcacagcctg
     1801 gaaaaaatat tataaactgg ccggcacata tatctttcat ttcaccattt acaagaacac
     1861 tcatcatgcc cttgcacata tatagtaaat aatagtcttt ccgtccagac ttattgtgag
     1921 ttgtaaaagg gtgaggtaaa acacagcaac ctgtacaatt tacaacaaga taacggtcat
     1981 taatctcttc gttacaccca gtactttccc aaatacgata atatgattga gcttccatag
     2041 catccaacat tttcccattt cctccctcaa tatatttcat gtttccgtat acagttgatt
     2101 tgtccatata aacaaccgat ttgtttattg tagtgtctcc aaacgtatta tacatttaat
     2161 tatagcaaca tttcaacagt tgttgccgga gaaataatag ctataaacaa taactaaaaa
     2221 cgatagctaa aaactttaac tgtaaatgat ggatgtatgt tatagctgta tgttatagct
     2281 attatgctaa actaagaaag aagccgtttc cgcttcaggt tgggtagcat tttcgaaagc
     2341 tttatttatt ttgctttcga aaaaattgta ctgccgtaaa tattgcaacc ggaaaggatt
     2401 cgctgcttag taagtattag aaaatgcaat gccaaataaa taatgaaaac tgttgaaaat
     2461 tacagaatga agggagaaaa tgttatgaaa aaagcaataa atgcttggtc agtacccaat
     2521 catattacct ttgcagaaac gtttcgtgat atttcatgtg caggatttga tggaatagaa
     2581 ttaaatctag acgcagataa ctattccagc cactcactta caatgggtat aggaaaagat
     2641 actttcaatg aaataaacag tctttcagaa gaatataacc ttaaggttgt cagtatttct
     2701 acctctctta ctgggggaaa attaggttct gataacaaat cagttcgtga atccggtaag
     2761 gatttaattc gaaaacaact ggaatgcgca gcagcacttg gtgcagatag tattcttaca
     2821 gtaccgggtg gtatgaccga tagcatttct ctgcttaaag catatgaaaa ctcatttatg
     2881 gcaattgaag agctcaaacc cgaaatagaa tctgcaaaaa tttatgttgg tcttgaaaat
     2941 gtctggaatg gtttttttac ttctccattt gatatgaaaa acttcatcga caaacttaat
     3001 tgtacataca ttggagcata ttttgatgtt ggaaatgtaa tagcattttc taatcctgaa
     3061 tattggattg aaattctaaa tacccgtata tgtaaaatac atgtaaaaga ttttatgcgt
     3121 aataatggta ttaatagagg agggtgtttt gtaaatcttc tggaaggtga tgctaattgg
     3181 aataaagtta ttactgaact ttgcaagatc ggttataatg gttttcttac agctgagctt
     3241 ggcatgatga tggaatgccc tgaatattta tacaaaatta catcaaaggc tcttgatata
     3301 ataattaatg ttaggtaata gtaatcaata ttaatcaata gtatttaagg tatttaatga
     3361 ttgatgtcgg tgtgtatttt ccctgaaggt actggtatta ggccaattat tatacaatta
     3421 caaaaactta taattagtaa ttaaaattat taatctgaag ttttgcattt ttattataaa
     3481 acctgatttg aaggcattta tatctgaaca tgaaagtagc tgatttgaag actttcaaca
     3541 aaaagtgtaa aaccatatta tgattgttcc ccagaaagcc gttttacgac ttttcactgc
     3601 aggattacct gcagtgagtt gcaactttgt tgcaacctgg cgcaatctaa tatggctatc
     3661 cagccttctt gcgaaggctg atgaaattca agatgcctta aatttcatca gaaagtgaat
     3721 tcatgacttt cagggtgata accatattat taataaaaaa ttaaaaaata gtttactata
     3781 ggaggtagtt atatgaaaaa gcttgcaatg ataggttgtg gaggaatagg gaaatatcat
     3841 ctaaatcatc ttttgagctt tagtgatatt gaattggctg gtttctgcga cccaattctt
     3901 gaacaggcag aaaaatttgt ttcagaagca ggaagtggta gagcatttac aaattataag
     3961 gaaatgtacg acaaaataaa ccctgatatg gtatttattt gtataccacc tgcagaacac
     4021 ggcgaggtcg aatttgaaac tattaaccgt cgcattccgt tttttgttga gaagccaatt
     4081 gcattatcca cggatcttgc caaacagatt tgttatgagg taaaaaaagc tgaactaatt
     4141 acagctgtgg gttttcaatg tcgttacagt aatcttgttg aaccaaatgt tagatttata
     4201 aaagaaaaag aaattatatt cgttgattgt atacgtattg gcagtgttcc cagagttgca
     4261 tggtggaaaa agcgttcagc ttccggaggt cagattgttg aacagacaat tcatcaattg
     4321 gatattataa gatatgtgct tgatgagccg aatgaggtgt ttacaatggg aacgaagggt
     4381 tttattaccg atgttgagga ttatgacact gaggatttgt caacaactgt tgtgcgattt
     4441 aaaagtggtg ctcttgctac tatttcgacc ggatgttatg ctacaagcag taatgctgca
     4501 gacagcaaaa ttgttttcag ttcccgtgat tcaagagcag aattacgtat tttagatgat
     4561 ctgaaaatat tcggtgatga tgaaattgca tataaagaga agggtgatgc cggtattctt
     4621 tgcgatcgtg cattcattga ttcggtacta acaagtgatg gttcaaaaat ccgctcttcc
     4681 tatgaagatg cagtatgtac tctggagttt gcattggcct gtaaccgttc aatggacaca
     4741 ggactaccgg tagaaatcgg ttcttaacct aggagtgcgc tcggaaagtc caagttatac
     4801 aagcagactg caagaagaac
//
LOCUS       DAOSDD010000299         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6280_length_4794_cov_3.666807, whole genome shotgun sequence.
ACCESSION   DAOSDD010000299 DAOSDD010000000
VERSION     DAOSDD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6280_length_4794_cov_3.666807"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1066)
                     /locus_tag="PK733_17845"
     CDS             complement(176..1066)
                     /locus_tag="PK733_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12431.1"
                     /translation="MALRFNEWQKGKKLNDFWLCYAISHCADYPELIDNIELNKSFGF
                     LGIRYLQINGLKLKNQSELAEYLINAKNAGIKSIDTTFYGLEEYHDKFAARKGDFTYL
                     LNILKIALELGIIPEPTFVILEDNKDQIVDLLSLLQKYIGVDAAIHGFLQDYRGNGEN
                     LENVRLTLQSYEAMSDKIKHYINVSGYKTEEDWLNGNSFSQYTSRNLILALRPDNIDM
                     LETMTCDEIINYIIELDERYYNVIPDIDVLAKFYGNKNCQKLYRQRDLLWKWQKQFIT
                     DNKLQLHDVTDERICGSMRF"
     gene            complement(1271..1675)
                     /locus_tag="PK733_17850"
     CDS             complement(1271..1675)
                     /locus_tag="PK733_17850"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOJ12432.1"
                     /translation="MGGVLVYHCCKKAGFSLPTRYPSGSCRFAGVYAWLEWARLDDVN
                     YFHSIDENSFMPERGDIVIYEKLITDESNDHIGIMLSCDDNNMLVAEGNKDSKNQSGV
                     IRRSRYDKILGFIRIDNNYQNKFTGVYDPKLD"
     gene            complement(1644..2156)
                     /locus_tag="PK733_17855"
     CDS             complement(1644..2156)
                     /locus_tag="PK733_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12433.1"
                     /translation="MLHYFYMYKTNIHEFHVQNGAEAVRLIIRDIGIFTKKEQKIILD
                     AIFHHSNKNIINDVYDEIVKDADVLQHYFYNVFKRVHENEATRLKRIINELLLPCDFQ
                     EAEEEKCSDEVSYCGKRKMLADSAETLASKTILGIPGDKDYCDICRYWPDDNIYKVLR
                     NGWCACLSLL"
     gene            complement(2430..2768)
                     /locus_tag="PK733_17860"
     CDS             complement(2430..2768)
                     /locus_tag="PK733_17860"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOJ12434.1"
                     /translation="MSRLIEKAVEDYTLVLSTYVVEELKSVVDRKFPSKMKAIEKFLT
                     ALSYELVYSHENYDGTLLFEIRDGKDYMVLHTAIIADVDILITGDKDFKDIGIERPEI
                     LTPKEFLDKY"
     gene            complement(2824..3087)
                     /locus_tag="PK733_17865"
     CDS             complement(2824..3087)
                     /locus_tag="PK733_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ12435.1"
                     /translation="MELAKITAKGQITIPIHIRKKLNLKDGDKVIFIEENGRVVMENS
                     TKVALREVQDAFSGEAERVGLETEDGVVDMVKQFRKEKLEKQL"
     gene            complement(3876..4034)
                     /locus_tag="PK733_17870"
     CDS             complement(3876..4034)
                     /locus_tag="PK733_17870"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12436.1"
                     /translation="MSHIFSSRLSSRPMGWSMVGIAKMSKLIIYKKNGGKIYYLIMAQ
                     KLKEAKNK"
     gene            4222..>4794
                     /locus_tag="PK733_17875"
     CDS             4222..>4794
                     /locus_tag="PK733_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOJ12437.1"
                     /translation="MNQQTQGNRIYAKSTKLFDPKIDFAFKQVFGQDNPECKKVLLHL
                     LNSTFQRKNGLGIKSIEYVNPYLDKVYDNDKESILDIKVKNENNELIDIEMQIASHTH
                     FIKRCLLYWASMYDKQLSEGEDYNKLKHCIVVSIVDFELDALAEMDDYHGVFELLERN
                     CYIKMTDHCELHFIQLPKVHFKCELKDTDNL"
BASE COUNT         1509 a          946 c          684 g         1655 t
ORIGIN      
        1 tgctactaat ccgtgattgc ggtattcggg caatacatcg attccgattg accacatttt
       61 tgaactccac caatatgctc ccactattcc tgcaattcta tctccatacc tccaatctca
      121 aatggtatca caacattaaa ttaaaccttt tatttgatgc acaaaaatca tgccactaga
      181 acctcattga tccacaaatc cgttcatctg ttacatcatg caattgtaat ttgttatctg
      241 ttataaactg tttttgccac ttccatagca aatctctctg ccgatatagc ttttggcaat
      301 tcttattgcc ataaaatttt gctaatacat ctatatcagg aataacatta taatatcttt
      361 catcaagctc aattatatag ttaataatct catcacaagt catcgtttcg agcatatcga
      421 tattgtcagg gcgtaaagcc aagataagat ttcttgatgt atactgagaa aaactgttgc
      481 cattaagcca atcttcttcc gttttatatc cggatacatt aatatagtgt tttattttgt
      541 ctgacatagc ttcataggac tggagtgtta acctaacatt ttctaaattt tccccatttc
      601 cgcggtagtc ctgtaagaag ccatggattg ctgcatctac acctatatat ttttgtagca
      661 atgataataa atctacaatt tggtctttgt tatcttcaag tatgacaaaa gttggctcgg
      721 gtatgatacc aagctctaaa gcgattttta aaatattaag caaatacgta aagtcacctt
      781 ttcttgcagc aaatttatca tgatattctt caagaccata aaaagttgtg tcaatgcttt
      841 taatccctgc atttttagca tttattaagt attctgccaa ttctgattga tttttaagtt
      901 ttaatccgtt aatctgtaaa taccggatgc ctaaaaatcc gaaagatttg tttaactcaa
      961 tattatctat aagttcaggg taatcggcac aatggctgat tgcatagcat agccaaaaat
     1021 cattcaactt ttttcctttt tgccattcat taaaacgcaa tgccattttc ttgcctcgat
     1081 agtatggaat cccatcactt gcttttttgc atgagcgaaa aaagcaatac ctacatgcac
     1141 atgcacaagg tatgttatga ttgattatca ttattcctct gttgaccatt ttatcaactc
     1201 ctctcgcaat taaaataatc atcagattat aataatgctt tatattttat agactgatta
     1261 ttagcaatat ttaatcaagc ttgggatcat atacaccagt gaatttattc tgataattat
     1321 tatctatccg aataaaacct aaaattttat catatctgct tctacgaatt acacctgact
     1381 ggttcttact atctttgttg ccttctgcaa caagcatatt gttatcatca catgacagca
     1441 taatgcctat atggtcattg gactcgtcag ttattaactt ttcgtaaatt acaatatctc
     1501 ctctttctgg cataaaacta ttttcatcga tactatgaaa atagttaaca tcatccagcc
     1561 gtgcccattc aagccatgca taaacacctg caaaacggca tgaaccacta gggtatctag
     1621 taggcagtga gaaaccggct tttttacagc aatgatagac aagcacacca cccatttctt
     1681 aataccttat agatattatc atcaggccaa taacggcata tatcacagta gtctttatcg
     1741 ccaggaattc ctaaaattgt ttttgaagcg agtgtctcag cagaatcagc aagcattttg
     1801 cgtttgccgc aataagaaac ttcgtcacta catttttctt cttcggcttc ctgaaaatca
     1861 cacggtagta ataactcatt tatgattctt ttcaggcgtg tagcttcatt ttcatgaact
     1921 cttttaaaga cattataaaa gtaatgttgt aaaacatcag catcttttac aatttcatca
     1981 taaacatcat ttatgatatt tttattactg tgatgaaaaa tagcatctag tattattttt
     2041 tgttcctttt ttgtaaaaat acctatatcc cggattatta gtcttacagc ttcagctcca
     2101 ttttgtacat gaaattcatg tatatttgtt ttatacatat aaaagtaatg aagcataccg
     2161 gcaatagctg caagttctgg gtcaagactt cttttttacg ccgacattac agcaaattca
     2221 gataccccat ataaatggag atacgcacag tgtttttctt ctgcatcaac catttgagtc
     2281 aacatttcat caaccactac cctaacgctt tccaaccgtt tgccaccttt agtaatcata
     2341 caaacatcat aataccgatt gtcaagggta ggtaataaga aacccggctt accgttttat
     2401 tggtttacag ggtattcctc tatttatgct taatacttat caagaaactc ctttggtgtc
     2461 aatatctcag gacgctcaat cccgatatcc ttgaaatctt tgtcacccgt aatcaatata
     2521 tcaacgtccg ctattatagc agtgtgtaaa accatataat ctttgccatc tcggatttca
     2581 aacaatagag taccatcata attctcatgc gaatatacta attcatatga taatgctgtt
     2641 aaaaatttct caatagcttt cattttactt ggaaattttc tgtcaactac cgatttcaat
     2701 tcttcaacaa catacgttga aagaaccaat gtataatctt ctaccgcctt ctctattaat
     2761 cggctcaagt tatcactccg aaaaagtatt gccaaatgag aacatttgta tcaagcatta
     2821 ttctcatagt tgcttctcca atttttcttt tctaaattgc tttaccatat ctacaacacc
     2881 atcttctgtt tctaacccca ctcgttcagc ctccccagaa aaggcatcct gaacctcgcg
     2941 cagagcaacc ttggttgagt tctccataac cacccgtccg ttttcttcga tgaaaataac
     3001 cttatctcca tcctttaaat tgagtttctt tctgatatga atcggtattg ttatctgccc
     3061 ttttgcagta atttttgcaa gttccatata tctaaactcc tttctatgaa ttccatgtat
     3121 tccttacttt tattatatgc tatttcgtaa catttatcaa cataaaacga aggccagata
     3181 gtgccaattt actgtaggga aaagataaca gataacactc tagtatccaa attattattg
     3241 ttatagtttt cagctttaat aatctttctt tattgattat agcataacag aaccattgcc
     3301 tacaactgaa ttaaatttaa catattattt aaagtacaac tttggtacta atttggtact
     3361 aattggatac aaaaagtacg aaatcagtac aataatggat atctctttac accactatga
     3421 aacagaagca taaaagcaat tgcacaaaat aattacaggt ataacaattt atacatgcga
     3481 aagcatgaaa tagtaataag tttttatcac aaattgttat atacctcttt atcgtctgac
     3541 taacatatat ctcaagacct ttcacggcat aaacggtttc cctcccggca aagccgggtc
     3601 ccttccattt atttcacggt gtcttgacct atatgttcca ccaagccact gaacaattgt
     3661 cgaaggatga agggaagaag aaagttaaat tcttgtttaa tcggtgctta aattatacta
     3721 caatgtgttt ttttacccac atctaccggt tatcgatctt aattcgttat ataatccggt
     3781 ttttcgccct gcttttacag caggtaggtt tctattccag acattttcat atccactcgc
     3841 cgttcccttt aattctctta ttaattcgtc ttgtattact tgttttttgc ttccttaagc
     3901 ttttgggcca taatcaggta atagatctta ccaccgttct ttttgtagat tattagtttg
     3961 gacatcttgg caatacctac cattgaccat cccataggtc tgctgcttaa tcggctcgaa
     4021 aatatatgac tcacatggcc ttctgcactg catccgacta tttcaaatcc ctttactgaa
     4081 cattctcagc aacacttgca taagtgtgaa ctgcttcttt gtgtcagcta attgtaattt
     4141 tataaaaatt tgatataata aaagtgatag aagatgtgtt ataagcagga aaatattatt
     4201 aataactatg gaggaaactt catgaaccaa cagactcaag gaaatcgtat atatgcaaaa
     4261 tcaacaaaac tgtttgatcc aaagattgat tttgccttta agcaagtatt tgggcaggat
     4321 aatcctgaat gcaaaaaagt actcttgcat cttttaaatt ctacgtttca gaggaaaaat
     4381 ggtttaggta taaaatccat agaatatgta aacccttatc ttgacaaggt atatgataat
     4441 gataaggaat caatactgga tattaaggtg aaaaatgaaa acaatgaatt aatagatata
     4501 gaaatgcaaa ttgccagcca tacacatttt attaagcgtt gtctgcttta ctgggcatct
     4561 atgtatgata aacagctttc tgaaggagaa gattacaaca aactaaagca ttgcatagta
     4621 gtcagtattg tagattttga acttgacgca ttagctgaaa tggatgatta tcatggggtt
     4681 tttgaattgc tggaacgtaa ttgttacatt aaaatgacag accattgtga gctacatttt
     4741 atacaactac ctaaagtgca ctttaaatgt gagcttaagg acactgataa tctg
//
LOCUS       DAOSDD010000300         4740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6365_length_4740_cov_5.222625, whole genome shotgun sequence.
ACCESSION   DAOSDD010000300 DAOSDD010000000
VERSION     DAOSDD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6365_length_4740_cov_5.222625"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1377)
                     /locus_tag="PK733_17880"
     CDS             complement(4..1377)
                     /locus_tag="PK733_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOJ12438.1"
                     /translation="MELNNDKNNNFEENMLERYTSLAKDSNYINPELYSKYQVKRGLR
                     DLDGRGVLVGLTEIGDVNAYIEVDNEMVPAPGRLTYRGIDIYSLVNGFLSHNQFGFEE
                     TAYLLLYGNLPKKCELDDFECLLSSYRTLPTGFVRDLILKAPSKDVMNGLSRSVLALY
                     SYDNNPEDTSIKNVVKQCMKLIALFPLLAVYAYQAYSHYHGANSLYIHSPKKDLSTAE
                     NILHMLRPDNKYTDLETRLLDLALVLHAEHGGGNNSTFVTHVVTSTGTDTYSAVASAL
                     GSLKGPRHGGANIKVVQMFENLKQNLKDWDDDDEIENYLLRMLNKEVFDCSGLIYGIG
                     HAVYSISDPRVEIFKEHVGELAKEKGLEKEYNLYMKVEKIAPEVIGKVRRIYKGVSAN
                     IDLYSGFVYRMLDIPQELFTPIFAIARIAGWSAHRIEEIVNAGKIIRPAYKSVAQKRE
                     YITVERR"
     regulatory      complement(1625..1774)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2079..2456
                     /locus_tag="PK733_17885"
     CDS             2079..2456
                     /locus_tag="PK733_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOJ12439.1"
                     /translation="MGMVVGSKVWFIPDGYYSSISNGKLFKSHEAICVLNPGKSDAHI
                     SITLYFEDRDCMSGFKALCKAERTNHIRMDNLKSERGYCVPMDVPYAMMVESDVEIIV
                     QYSRMDTSQAEMALMTTIAYPFK"
     gene            complement(2619..3587)
                     /locus_tag="PK733_17890"
     CDS             complement(2619..3587)
                     /locus_tag="PK733_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12440.1"
                     /translation="MELIDTDKKTKKKVISANNDREKKVLIAKPCARAADVLDFYKIS
                     TLIVVVLFAVVTAIPFIIMLSVSLQTMKEIYSPELAFIPKELQFVNYAKAMKNGAWGR
                     YLFNSVYVTVIVVIISLLINSMTGYVFARIRFKGRNFLFILLLVGMMIPPQVTMVPLF
                     IMMKKFPLAGGNNIFGAGGKGLINTYAGLIIPFIAGSFGVFLCRQFYMTFPQDIDNSA
                     RIDGCSRFMTFIKIYIPLSKPVLASLGILKFTGTWNEYNWPLIMTNSDNMKTVQIALT
                     MFRDEAEILWNQLMAATIVSSLIIYALFLCLQKYFVAGILAGSVKE"
     gene            complement(3608..4606)
                     /locus_tag="PK733_17895"
     CDS             complement(3608..4606)
                     /locus_tag="PK733_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ12441.1"
                     /translation="MQAKRRDGLNQIYQKKEVIEDSFFKGRASKSRASGNKVLKNRLS
                     KDDREAYIMISPFFIFFVLFVLVPITVNFVNSFTNYNLGKTRGFVGLENYIKLFVDRD
                     FLRSLWNTLIYAAFSVIPLTVLGFITAVAVNGRAKIFYITRALFVFPYVTSMVAVSMI
                     WLYMYEPTTGVFNKILISLGFEPLKWLFDEKLALLCLIIMNIWKNIGYVMIIYLAGLQ
                     SIPENLYEAGKVDGASEFKMLFKITIPMVRSISFFIFVTTSIESFKTFEQVRIMTGGG
                     PVNSTTTIVHQIYLRAFSEYKMGYASAISIVLLIIIFAITMMNMKLGKGKGQNDEI"
BASE COUNT         1504 a          927 c          735 g         1574 t
ORIGIN      
        1 cacttatctc ctttctacag taatatactc acgcttttga gcaacacttt tgtacgccgg
       61 tctgattatc tttccggcat ttacaatttc ttctatacga tgtgcactcc agcctgcaat
      121 tctggcaatg gcaaaaatcg gtgtaaataa ttcctgcggg atatcaagca tcctgtatac
      181 aaagcccgag tacagatcaa tgttagcact gacgccttta tatattcttc gcacttttcc
      241 tatcacttcc ggcgcaattt tttccacttt catataaagg ttatattctt tctccagacc
      301 cttttctttt gccagttctc ccacatgttc cttgaatatt tctactcttg gatcagatat
      361 ggaatataca gcgtgaccaa tcccataaat caaaccggaa caatcaaaga cttccttatt
      421 aagcattctt aacaggtaat tttcaatttc atcgtcatca tcccagtctt taaggttttg
      481 cttaaggttt tcaaacattt ggacaacttt aatgtttgca ccgccatgtc ttggtccctt
      541 taaagatcct aaagctgatg caaccgcaga ataggtatct gttcctgtgg aggttactac
      601 gtgggttaca aaggttgaat tatttcctcc cccatgttct gcatgcagca caagtgcaag
      661 atcaagcaat cttgtttcaa gatctgtgta cttgttgtca ggtcttaaca tgtgcaggat
      721 attctcagct gtacttaaat cttttttggg actgtggata tataggctgt ttgccccatg
      781 atagtgggaa tatgcctgat atgcataaac tgccagcaga gggaaaagtg caattaactt
      841 catgcactgc ttcaccacat tttttattga agtatcctct gggttgttat catagctgta
      901 taacgctaaa acactcctgg ataacccatt cataacatcc ttacttggag cttttaaaat
      961 cagatcacgg acaaatcccg ttggtagtgt acggtatgaa cttaaaaggc attcaaaatc
     1021 atctagttca cattttttag gtaaattgcc ataaagtaat aaatatgctg tttcctcaaa
     1081 tccaaactga ttatggctta aaaacccatt gaccaggcta taaatatcaa tgcctctgta
     1141 tgttaatctt ccgggggcag gtaccatttc attgtcaact tcaatatatg catttacatc
     1201 tcctatctct gttaaaccaa ccagcactcc ccttccgtcc aggtcgcgaa gacctctttt
     1261 tacctgatac ttactatata actcagggtt tatataattg ctatctttag ctaaacttgt
     1321 atatctttca agcatatttt cttcaaaatt attattctta tcattattca attccatatt
     1381 tctttacctc cccttttgat aataatgtaa atgaaaaagt aaatatcatg tatatatgct
     1441 acttttaacc ataatattgc taagttaata tgtaatatta ttccagcaca aattcagcat
     1501 aaatgtagca taaatcatac aagaaatcat gattcatttt aattaggatt cctatttaat
     1561 ttttacttat taaaaattaa atctaaatct aaaataagaa gtctgtgaat aaaagaacaa
     1621 aaaccctcct tcccttcaac gcttccgagg ttagctgacg gattcggact gaaaggaatt
     1681 agccctacca ggattttaac atcccggatt caccccataa aattggttcc cccgtatcct
     1741 gcataaataa gcagaaattc agcaaataaa tctgtagatt gcttcagcta acagataagc
     1801 cttcagccta ttgacaaaat tcataagctt tcagctaatt acctgtacca ttacattttt
     1861 atttaattac attttataat aacacttttc ccattataat gcaaatagaa ttttttataa
     1921 tacatttgtc aatttccgca atatgtttgg tacaatggtt gaaactcaat cgtgaaaatg
     1981 ggtataatat ctatatgtta atatcactgc tgcattggca gtatttgttt tgattttttg
     2041 caattccagc aataattctt gaagggggat ttattaaaat gggaatggtt gtcggatcaa
     2101 aagtatggtt tatacctgat ggctattatt catcaatcag taacggtaaa ttatttaaaa
     2161 gccatgaggc aatttgtgtt ttaaaccctg ggaaaagtga tgcccacata tctattactc
     2221 tgtattttga agacagagac tgcatgtctg gctttaaagc tctttgcaag gctgaaagaa
     2281 caaaccatat tcgtatggat aatttgaaaa gtgaacgtgg gtattgtgtt cccatggacg
     2341 ttccttatgc aatgatggtg gaaagtgatg tggaaataat tgttcaatac agcaggatgg
     2401 atacttctca agctgaaatg gctttgatga ctacaatagc ttatccgttt aagtaaaagt
     2461 tcaataaaaa aatcacagta cttttgtaac cagtatagtg tttctcaaat acctatacaa
     2521 atgaaaaacc tgcgaaaccg taaaatcatc gggattctcg tcttttcagg aaaatgaggt
     2581 aatttgagaa cacactacta taagtggtta taactctttc attcctttac actgcctgca
     2641 agtatgcctg caacaaagta cttttgcaag cataaaaata gagcataaat aatcaagctg
     2701 gaaacaattg ttgcagccat aagctgattc cataaaatct cagcttcgtc cctgaacatt
     2761 gtcagcgcaa tttgaacagt tttcatatta tcggaattag tcataattaa cggccaatta
     2821 tattcattcc atgttcctgt aaattttaat atgccaagag aagccaggac aggtttagac
     2881 aatggtatgt atattttaat aaaggtcatg aatctgctgc agccatcaat tcgtgctgaa
     2941 ttatcaatat cctgcgggaa agtcatatag aactgacggc aaaggaatac accaaaagaa
     3001 cctgcaataa aaggtattat aagtcctgca taggtattta tcagtccctt tcctcctgcg
     3061 ccaaatatat tgtttccacc tgcaagagga aattttttca tcataataaa aagaggaacc
     3121 atagttacct ggggtggaat catcataccc accaaaagca gtataaacaa aaaatttcta
     3181 cccttaaacc tgatgcgggc aaatacatat cctgtcatgg aatttatcaa aaggctgata
     3241 attactacaa ttactgtgac ataaacgctg ttaaatagat atcttcccca tgctccattt
     3301 ttcattgctt ttgcataatt aacaaattga agctcctttg ggataaatgc caattccggc
     3361 gaatatattt ctttcattgt ctgcaaagat acagaaagca tgattataaa aggtatagca
     3421 gtaacaactg caaacaatac tactacaatc aaagtagata ttttataaaa atctagtaca
     3481 tcagcagctc tggcacaagg ttttgctatc aaaacctttt tttccctgtc attatttgca
     3541 cttattactt tcttcttggt ttttttatct gtatctataa gctccattta taagctccat
     3601 ttctatatca gatttcatca ttttgacctt ttcccttccc aagcttcata ttcatcattg
     3661 taattgcaaa aataattatc aaaagcacta ttgatattgc tgaagcatac cccatcttgt
     3721 attcgctgaa tgcacgcaaa taaatctggt gtacaattgt ggtagtactg tttactggtc
     3781 caccgccagt cataatccgg acttgttcga aggttttaaa tgattctata ctggttgtaa
     3841 caaatatgaa gaatgaaata gaccgcacca tgggtatggt aattttaaac agcattttaa
     3901 attctgaggc tccgtcaact tttcccgctt catataagtt ttccggaatg ctctgaagcc
     3961 ctgcaagata aattatcata acgtatccta tgtttttcca tatgttcata ataatcaagc
     4021 aaagcaatgc aagcttttca tcgaacaacc actttaaggg ttcgaatcct agtgatataa
     4081 gtatcttgtt aaatactccc gtcgtaggtt catacatata tagccagatc atggaaactg
     4141 caaccataga agttacatag ggaaatacga aaagtgccct tgttatatag aagatttttg
     4201 cccgtccatt aactgcaact gctgtaataa accccaaaac cgtaagtggg attacactga
     4261 atgcagcata aatcaaggta ttccacagag aacgaagaaa atctctgtcc acaaacaact
     4321 taatatagtt ttcaagacct acgaatcccc ttgttttccc aagattgtaa tttgtaaagc
     4381 tgttgacaaa atttacagtt atgggaacca acacaaagag aacaaaaaag ataaaaaagg
     4441 gtgatatcat gatgtaagct tccctatcat ctttgctgag tctgtttttc aagactttgt
     4501 ttccggaagc tctgcttttc gaagctctac ccttgaagaa gctgtcttca ataacctctt
     4561 ttttttgata tatttgattt aaaccatcac gcctttttgc ctgcatacta atttatatca
     4621 cccttaatta ttttttcttt cttaatttat tcattcatta attaattata ttatcaattt
     4681 attactttac ccagagtgca tattttattt ttgaagaaat tattgaaaga agcattagtg
//
LOCUS       DAOSDD010000301         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6449_length_4688_cov_5.217785, whole genome shotgun sequence.
ACCESSION   DAOSDD010000301 DAOSDD010000000
VERSION     DAOSDD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6449_length_4688_cov_5.217785"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            669..3173
                     /gene="infB"
                     /locus_tag="PK733_17900"
     CDS             669..3173
                     /gene="infB"
                     /locus_tag="PK733_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ12442.1"
                     /translation="MQDERKTEALKQTNIKDKGTKESRANGEVMVNETNKSSGVVEKG
                     KSDKTRKWDERPRGKFNDKNYTKTISGDNKMKEKPESRSNPGKNSLDRHADNTNRSSD
                     KNKYTNNIRQEEKKTNIGDSVKREVYKGNRPNKLNIPKEILETPKNEEDVRIDENKRE
                     YQNKDLDKDIKREQKREIPKTKPIEDKKKFKPRNIVLDSSKGVSQLLQEDELVIDELL
                     DSTAERKTRKPARQRREQRKNSDASKAAVERAVLTSIKIGESITVKELAENLKKTAAD
                     VIKKLMNMGVMATLNQDVDYDTAVIIADEYGVKVEKEVVINEEDILFDDEQDDEKDLV
                     PRPPVVVVMGHVDHGKTSLLDAIRKTNVIQSEEGGITQHIGAYTVKINDRNITFLDTP
                     GHEAFTAMRARGAQITDIAILVVAADDGVMPQTIEAINHAKAAGVTIIVAINKIDKPA
                     ANPDRIKQQLTEYGLVAEDWGGDIIMVPLSAKKHQNIDLLLEMVLLTADIMELKSNPD
                     KQAKGTIIEAQLDRNRGPVATLLIQRGTLNVGDSIITGTIFGRIRAMTDDRGHSIKTA
                     GPSTPVEILGLPEVPESGELFYAVKDEKLSKTLVDKRKQQHREEQLKSNSMISLEDLF
                     NQIKQGQVKELNIIIKADVQGSVEAVKQSMEKLNNEEVRINIIHGGVGAVTETDVKLA
                     EVSNAIIIGFNVRPGPNVSETAENSGVDIRLYRIIYDAINDIEAAMKGMLEPKLKEVI
                     QGHAEIRQIFKASGIGTIGGSYVTDGKITRNSGVRIVRDGIVVYEGKLASLKRFKDDA
                     KEVAQGYECGILIERFNDIKEGDIVEAYAMEEVENK"
     gene            3266..3595
                     /gene="rbfA"
                     /locus_tag="PK733_17905"
                     /pseudo
     CDS             3266..3595
                     /gene="rbfA"
                     /locus_tag="PK733_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
     gene            3725..>4688
                     /locus_tag="PK733_17910"
     CDS             3725..>4688
                     /locus_tag="PK733_17910"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOJ12443.1"
                     /translation="MTLHDIISALKKSKSIIILPHISADGDALGSGLALALALKRQNR
                     GNGKDIKLYIEEAIPRMYSFLPDREIVEVYSDKHRTPVKNADNNITDVYETVIAIDTG
                     DMERLGKRAEIFNNAELKINIDHHATNTLFGNINYVNEEASATGEIIYQLIKMMGSDI
                     DRDIALCLYVAITTDTGGFRYSNTTPLTHLITSDLIGYGINIADVSFRVFDSISEGKL
                     KLMGMAINSLELYEQKKIAFITVTEKMIEEAGAVEEESEGLVNLGRNINTVEVAALFK
                     LKPNDEIKISMRSNSYIDVSKIASRYNGGGHKKAAGCTIMGNLND"
BASE COUNT         1813 a          639 c         1077 g         1159 t
ORIGIN      
        1 caccgagaat aattaaaaca acagaagttt ttgttgactc ggataatgat agaaaacgta
       61 gagacaatgt tggcaatttc agggataacg accgtagaaa aagaaatggg cagtatgaaa
      121 gagataaagg tgaacctcgt ggtggaagaa aatatagcgg aataaaagtt agtactgcag
      181 gatcaggttt aagggcaggt tttgttagag atacaggcta tgcaattccc cctgtaaaca
      241 aaaaaacaga ttcagggagt attggagaga caagtgccgg acaaaaacct gaattaaaac
      301 ctgaagtgaa acttgaatta aaacctgaag tgaaacctga attaaaacct gaagtaaaac
      361 ctgaggtgaa acctgagaaa aagcctgaga aaaaacctga ggcaatgctt gagacaaaac
      421 ctgaagtgaa gcttgaagtt aaacccgagt taaaatctga ggataagtct caattaaaat
      481 cggaaataat acatgataaa aaagttgagg gaaatattga acccaaaatt gaatcaaaga
      541 ctaaagcaaa tattaaagga atcggaattt ctgatcagag caatataaaa tatgaaggga
      601 aagatgaagt aataacaggg aaggaacaag caactaatat aaaaaggaat tataatgata
      661 agaaaaccat gcaggatgaa agaaaaacgg aagcgttaaa acaaactaat ataaaagata
      721 agggtactaa agagagtaga gcgaacggtg aagtcatggt aaacgagaca aataaatcca
      781 gtggagttgt tgaaaaggga aaatcggata aaaccaggaa atgggatgaa aggcctagag
      841 gaaagtttaa tgataagaat tataccaaaa ccatatccgg ggataataaa atgaaagaaa
      901 aacctgaaag ccgttctaat ccgggaaaaa atagtttgga taggcatgct gataatacta
      961 acaggagtag tgataagaac aaatatacta ataacatcag acaagaagag aaaaaaacaa
     1021 acattgggga ttcagtgaaa agagaagtct acaagggaaa tagacctaat aaattgaata
     1081 taccaaaaga aattttggaa actccaaaaa atgaagaaga tgtacgtata gatgagaata
     1141 agcgcgagta tcagaataag gatttagata aagacatcaa gcgtgaacag aaaagagaaa
     1201 taccaaaaac aaaacccata gaagacaaaa agaagtttaa acctcgcaat attgtactgg
     1261 attcttcaaa gggtgtatct cagctgttgc aggaagatga actggtaatt gacgaacttt
     1321 tggattcaac tgcagaaaga aaaacaagaa aacctgcaag gcaaaggaga gaacagagaa
     1381 aaaacagcga tgcatcaaaa gctgctgttg aaagagcagt ccttacatca ataaagatag
     1441 gcgaatcaat tactgtaaag gaactggctg agaacttaaa gaaaacagca gcggatgtga
     1501 ttaaaaagtt aatgaacatg ggtgttatgg ctacgctaaa tcaggatgtt gactatgata
     1561 ctgctgtaat aattgccgat gagtatgggg ttaaagtaga gaaagaagtt gttataaacg
     1621 aagaggatat tttgtttgat gatgaacagg atgatgaaaa agatcttgta ccaagaccac
     1681 cggtagttgt tgtaatgggt catgtagacc atggtaagac atcacttctt gacgctatta
     1741 gaaagaccaa tgttattcaa agcgaagagg gtggaattac acagcatatt ggtgcttata
     1801 ctgtaaagat aaacgatagg aacattacat tcctggatac accagggcat gaggctttta
     1861 ccgccatgag agccagggga gctcaaataa ccgatattgc aatacttgtt gttgctgccg
     1921 acgatggtgt aatgccccaa actattgagg ccatcaacca tgctaaagct gccggggtaa
     1981 caataattgt ggcgattaat aagattgata aacctgctgc aaatcctgac agaataaagc
     2041 agcagttgac agaatatggt cttgtcgcag aggattgggg cggagatatt attatggtac
     2101 ctttatctgc taaaaagcat cagaatattg atttgcttct tgaaatggtt ttacttactg
     2161 cagatataat ggaattaaag tccaaccctg ataagcaggc aaagggtact ataattgaag
     2221 cacaactgga taggaataga ggtcccgttg ccacactttt gatacaaaga ggtactttaa
     2281 atgtaggtga ttcgattatc acgggaacaa tattcggaag aataagggca atgactgatg
     2341 atagagggca tagcattaaa actgcagggc cttctacgcc tgtagaaatt cttgggttac
     2401 ctgaagttcc ggagagtggt gaattgtttt atgcagtgaa agacgaaaag ctttcgaaaa
     2461 cactggtaga taaaagaaaa cagcagcata gggaagaaca gcttaaatca aattcaatga
     2521 tttctctgga agatttgttt aaccaaatta agcaaggaca ggtaaaggaa ctcaatatta
     2581 taataaaagc tgatgttcag ggttcagttg aagcagtaaa gcaatctatg gaaaaactga
     2641 acaatgagga agtacgaatt aatataattc acggtggggt aggtgctgta acggaaactg
     2701 atgtaaaact ggcagaagta tcaaatgcca ttattattgg ttttaatgtc agaccgggtc
     2761 ctaatgtaag tgaaactgca gagaattcag gcgtggatat tagattatac agaataattt
     2821 atgatgctat taacgatata gaagcagcaa tgaaagggat gctggaacca aagcttaaag
     2881 aggttattca gggtcatgca gaaataagac agatctttaa agcctcggga ataggcacaa
     2941 taggtggttc ttatgtcacc gatgggaaga ttacaagaaa ttccggagtt cgtattgtta
     3001 gagatggcat tgtggtatac gagggcaaac tagcatcatt aaagaggttc aaagatgacg
     3061 ccaaggaagt tgctcagggt tatgaatgcg gaatactcat tgagaggttt aatgatatta
     3121 aagaagggga tattgttgaa gcttatgcca tggaagaggt agaaaacaaa taggaaaatc
     3181 cgccttacat tatttaggcc atgatatgag ggaaaatatg aaaatcgccg caatgagctg
     3241 caatgaatta aggagtgatt gatctatggt tgatagaata aatagaatat cagaggaagt
     3301 aaaaagggaa atcagcaata taattcaagg tgagctaaag gatccgcgtt tgccggaatt
     3361 tgtaagtgta acatcagtga atgtaactcg tgacttaaga tatgcaaaag tatatattag
     3421 catattggga aatgacgaag aaaagaaagc agcactgcaa gggttaaaaa atgctgcagg
     3481 ttttataaga cgtgaaatag gccagagaat aaaattaagg tatacacctg aactacagtt
     3541 tgaacttgat aattcaattg aacatggcat ttatatcagt aaactaatta atgatgttat
     3601 aaagaatgat gaaaaaaata atgaaactga tgatatgact gatgaagaaa cagatgatgt
     3661 agctgatgat attaatttta cagatgatga caaaggtaat tagatattag cagctggagg
     3721 ttatgtgaca ttgcatgata taattagtgc tttgaagaaa tcgaaaagta taataatact
     3781 tcctcatata tctgccgatg gagacgcact agggtcaggg cttgcattgg cccttgcgct
     3841 aaaacggcaa aatcggggga acggcaaaga tataaaacta tacattgaag aggcaattcc
     3901 tcgaatgtat agttttttgc cagaccggga aattgttgag gtgtactcgg ataaacatcg
     3961 tactcctgtg aaaaatgctg ataataatat tactgatgtt tatgaaacgg taatagctat
     4021 agatacgggt gatatggaaa ggctcgggaa aagagctgaa atatttaata atgcagagtt
     4081 gaagattaat attgatcacc atgcaaccaa cacattattt ggcaatataa actatgttaa
     4141 tgaagaagct tctgctaccg gtgaaattat ctatcaattg ataaaaatga tgggttctga
     4201 cattgataga gatattgcat tatgtttgta tgttgcaata actacagata caggagggtt
     4261 cagatacagc aatacgacac cactgacgca tttaattact tccgacctga ttggttatgg
     4321 aattaatatt gcggatgttt ctttccgggt ttttgactct atatcggaag gtaaactcaa
     4381 attaatggga atggcaataa attcacttga gttgtacgag caaaaaaaaa ttgcgtttat
     4441 aactgttact gaaaaaatga tagaggaagc cggggctgtt gaagaagaat cagaaggtct
     4501 ggttaacctg ggtcgcaata taaatactgt tgaagttgcc gctttattca agctaaaacc
     4561 aaatgatgaa ataaaaataa gtatgagatc caattcatat attgatgttt caaaaattgc
     4621 ctccagatac aatgggggag gtcataaaaa agctgcaggt tgtaccataa tgggcaattt
     4681 aaatgatg
//
LOCUS       DAOSDD010000302         4641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6518_length_4641_cov_5.247274, whole genome shotgun sequence.
ACCESSION   DAOSDD010000302 DAOSDD010000000
VERSION     DAOSDD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6518_length_4641_cov_5.247274"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK733_17915"
     CDS             complement(<1..199)
                     /locus_tag="PK733_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12444.1"
                     /translation="MLKKIISMLIIVILVTGIFLTVGPLTPNPISPQTVTAEDLRGGF
                     QLIPESMDSTGVKPDSSYMLKS"
     gene            442..1584
                     /locus_tag="PK733_17920"
     CDS             442..1584
                     /locus_tag="PK733_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOJ12445.1"
                     /translation="MQKLHLIATAAFGIEAVVGRELKKLGYADQAIDNGRVTFAGDFE
                     AICRTNIWLRSAERVLVKVGEFKATSFEELFENTKMLPWEEWIPEDGEFPVEGKSVDS
                     KLASISDCQAIVKKAVVERLKQKYRRDWFDETGPKYKIEVGLLKDIATLTIDTTGAGL
                     HKRGYRKLVSSAPVRETLACAMLQVSRWNPDRVLLDPFCGSGTIPIEAALIGKNIAPG
                     LKREFTAESWKIIPQGLWKNAREEAMDSIKPDRELRIQGTDIDENVLSLARYHAKLAG
                     VEDSIHLQRLPVADISSRYKYGFIICNPPYGERLGELREVEKLYTQMGNVFKKFDTWS
                     YYILSSHPDFERFFGRRADKKRKLYNGMIKCNYYQYFGPPPIRSER"
     gene            1797..3272
                     /gene="aroA"
                     /locus_tag="PK733_17925"
     CDS             1797..3272
                     /gene="aroA"
                     /locus_tag="PK733_17925"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ12446.1"
                     /translation="MLLKVDKSKIKSGSIISIPGSKSHTIRALFIASLASGRSEIMHP
                     LVSDDAFSAVEVCKALGAKVTEHNFKVYSEECSRLGLRERTFKVEGYAPKILGVECPR
                     SANVREHALKVSDMGCSQSRECPLLIVEGFDGVPSIPDDVINVGNSGTTLRFGVTAAA
                     LSEGYTVFTGDGQIRNRPMEPLIKACNNLGAKVFSTRNNGMVPVVVKGRMKGGITEID
                     AYTSQFLSSLLVTCPLLEKDTEIIVTQLNEKPYVEMTLWWLDQEGITYSTNNFKSIYI
                     QGGQKYKPLKVKIPGDFSSATFFMVLAAISRADITLENLDITDPQGDKLILSFLENMG
                     AEVSYNSYNKDGVNSNSINIKGGNLCGIEIDMNSTPDAIPAMAVAGCFAEGETKLLNV
                     PQARLKETDRISVMCRELRKMGADITELPDGLIVRESRLKGCEVNGHYDHRVVMALAI
                     AGLNAEGTTIIDTAEAINVTFPDFGELVKNCGGGIEFVEEM"
     gene            3414..4595
                     /gene="aroC"
                     /locus_tag="PK733_17930"
     CDS             3414..4595
                     /gene="aroC"
                     /locus_tag="PK733_17930"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOJ12447.1"
                     /translation="MMGNSFGRLFRITTCGESYSGGFRKFSDIPEQLRGGLITIVDGV
                     PPGVRITPELIQDELDKRRPGLSKLSTPRKEMDKVYIFSGIMEDYYTTGAPVGFVIPN
                     SDIKDYHIEEHRAGKDYIRPGQAAYTYYKKYGQFADWAGAGRASGRETASRVAGGAVA
                     KVVLDAMGIDVIAYVIESHGISCGPVTYETAKANYRKNEINCPDLDKGEEMIQDLLKV
                     KAEGDSCGGIVEIIVKGLPAGIGEPVFDKLDAVIAHGLMSIGAIKGLEFGAGFENARM
                     KGSESNDTPYYDEESGRIRFKTNRAGGILGGISNGEEIRARVAVKPTPTIMKEQETVD
                     VDMQKNINYKFTSRSDPSICPRIWPVCEAMVRITLLDAILMAKAYRAISFNLDPKWDL
                     L"
BASE COUNT         1492 a          720 c         1120 g         1309 t
ORIGIN      
        1 gagacttaag catataactg ctgtcaggtt taacacctgt agaatccata gattccggta
       61 taagctggaa tccgcccctt aagtcttctg cagttactgt ttgaggagaa atgggatttg
      121 gggtcaaagg accgacagtg aggaatattc ctgttactaa gataactatt attagcatac
      181 ttataatttt ttttaacata aattgatcct cccggatatt atatttattt tattttgtta
      241 aaatccaagt tattgccgta aaataaacat gatgaaatat gtacaatgat tagactacgg
      301 agattggtgt tttgttccta ttattgacct tgataaatct acaataaact gataaagtag
      361 caaaatagga aagtaataaa ataggattag gattatactt tgatagatta tgaaatagtt
      421 taaactttat gggaggaact tatgcagaaa ctgcatctta ttgctactgc tgcttttggc
      481 atagaagcgg tagtaggaag ggaacttaaa aagcttggct atgcggacca ggcaatagat
      541 aatggaaggg ttacatttgc cggggatttt gaggcaattt gcaggacaaa tatttggctt
      601 agatccgccg agcgagtact ggtaaaagtt ggagagttta aggcaacttc atttgaagag
      661 cttttcgaaa atacaaagat gctgccttgg gaagaatgga ttcctgaaga tggagagttc
      721 ccggtagaag gcaagtcggt agattcaaag ctggcaagca tatctgattg ccaggccatt
      781 gtaaaaaagg cggttgtgga acgattgaag caaaagtata ggcgggactg gtttgatgaa
      841 acgggaccta aatataaaat tgaagtgggt ctccttaaag acatagctac cttgacaata
      901 gataccaccg gagcgggact tcataaaagg gggtatcgta agcttgtgag cagtgcacct
      961 gtaagagaaa ctttggcttg cgccatgctt caggtgagca ggtggaatcc ggacagagtc
     1021 cttttagatc cattctgcgg ttcaggtaca ataccaattg aagcggcatt gataggtaaa
     1081 aatattgctc ctggattaaa gagagaattc acagcagaat cgtggaaaat aatccctcaa
     1141 ggactatgga aaaatgccag ggaagaggct atggattcga taaagccaga cagagaatta
     1201 agaatacagg ggactgacat agacgaaaat gtacttagtc ttgcacgtta tcatgcaaaa
     1261 ttggcaggag tggaggacag tattcattta cagcgtttgc ctgtagcgga tataagttca
     1321 agatacaaat atggtttcat tatatgcaat cctccatatg gtgagcgatt gggtgaacta
     1381 agggaagttg aaaaactata cactcaaatg ggaaatgtat ttaagaaatt tgatacctgg
     1441 tcctactata tattatcatc ccaccctgac tttgaaagat tctttggccg cagagccgat
     1501 aaaaagcgga aactatataa tggcatgata aagtgtaatt attatcaata tttcgggcct
     1561 ccgccaatta ggagtgaaag gtgagattta tgcgcccctt aagtagggtg catattttat
     1621 ttttgaagaa attattggaa gaagcattag tgaaggctct aagtaccgta acgatagctt
     1681 ctggaatata tttcttcaaa aattttaaat gcaccccctt aagtaaacaa tcttgactta
     1741 tattactcta tataatatta ttttataaag taggatggta gaaagggtta tgcatgatgt
     1801 tgctaaaagt ggataaatct aaaattaaat ccgggagcat tataagtatc ccgggttcta
     1861 aatctcatac catacgggca ttatttattg caagtcttgc ctcaggcaga tcggaaataa
     1921 tgcatcctct ggtttctgac gatgcatttt cggcagttga agtttgtaaa gctcttggag
     1981 cgaaagtaac ggaacacaat ttcaaagtct atagcgaaga gtgttctcgg cttgggttga
     2041 gggaacgcac ctttaaagtc gagggatatg ctccaaaaat ccttggtgta gaatgtcccc
     2101 ggtctgctaa tgtaagggaa cacgccttaa aagtctctga tatgggatgt tcccagtcta
     2161 gggaatgtcc tcttttgatt gttgaaggat ttgacggcgt gccttcaata cctgatgatg
     2221 ttattaatgt aggtaattcg gggacaactt taaggtttgg tgtaacagca gcagctttga
     2281 gtgagggcta tactgttttt acaggggacg ggcaaataag gaataggccg atggaacctt
     2341 tgattaaggc atgcaataat cttggagcaa aggtgttttc aacaagaaat aatggaatgg
     2401 tacctgttgt agtaaagggg agaatgaaag gcgggataac tgaaattgat gcctatacat
     2461 cccagtttct ttcatcgtta ttggtaacct gtcccttgct ggaaaaggac acagaaataa
     2521 ttgtcacgca gttaaatgaa aaaccatatg ttgaaatgac tctgtggtgg cttgatcaag
     2581 aaggaataac gtattccact aataacttca aatccatata tattcaaggc ggccagaaat
     2641 ataagccttt gaaagtaaaa attcccggtg acttttcttc agcaactttt tttatggttc
     2701 ttgcagcgat ttcgagggct gatattacac tggaaaattt ggatattaca gatccccagg
     2761 gggataagct tatattgtct tttctcgaaa atatgggagc tgaggtttca tataattcat
     2821 ataataaaga tggagtaaat agtaatagca taaatattaa aggtggcaat ttgtgtggca
     2881 ttgaaataga tatgaactcc acacctgatg ctatacctgc catggctgtt gccggctgtt
     2941 ttgcggaagg tgagacaaaa cttttaaatg taccccaagc cagattgaag gaaactgaca
     3001 ggattagcgt aatgtgtcgg gaattaagaa agatgggtgc ggacataact gaattgcctg
     3061 atgggcttat tgtaagagaa agcagactaa aaggctgtga agttaatggt cattatgacc
     3121 atagagttgt aatggctctt gccatagctg ggctaaatgc ggaaggcact actataattg
     3181 atacagctga agctataaat gtaaccttcc cggattttgg agagcttgtt aaaaactgtg
     3241 gtgggggtat tgagtttgtt gaggaaatgt gatcatctta aagcataaat gtaaaacatt
     3301 agctttggta gctgtgttgg ttgttatgcc ggtagttagg ccggtgacta ttccaataat
     3361 tgtaattttg ttgaatttgg ctttgcagaa tagctaaagg aaggtgtgtt aacatgatgg
     3421 gaaatagctt tggacgctta tttagaataa ctacgtgcgg cgaatcctat agcggcggat
     3481 ttcgtaaatt ttctgatata cctgaacagc taagaggtgg gcttataaca attgttgatg
     3541 gcgtacctcc aggagtaaga ataacacctg aattgataca ggatgaattg gataaaagac
     3601 gtcctggttt atcaaaatta agtactccga gaaaagaaat ggacaaggtt tatatatttt
     3661 caggaataat ggaggattat tatacaacgg gggcgccggt agggtttgtt atacctaata
     3721 gtgatattaa ggattatcat attgaagagc atagggcggg caaggattat atacggccgg
     3781 gacaagctgc ctatacctat tataaaaaat atgggcaatt tgcagactgg gcaggggcag
     3841 gacgtgcttc aggccgagaa acagcttccc gggtagcagg aggagccgta gccaaagtag
     3901 tattagacgc aatgggaatt gatgtaattg catatgttat agagtctcac ggcataagtt
     3961 gcggacctgt tacatatgaa acagcaaagg ctaattaccg gaaaaatgaa attaattgtc
     4021 ctgatcttga taaaggagaa gagatgatac aagaccttct taaggtaaag gcagagggtg
     4081 attcctgcgg aggaattgtg gaaataatag ttaaaggctt acctgcagga ataggagaac
     4141 ctgtgtttga caagctcgat gcggtaattg cccatggact tatgtctata ggagcaatta
     4201 aaggacttga atttggcgcc ggttttgaaa atgcaagaat gaaaggatct gaatccaatg
     4261 atacaccgta ttatgatgag gagtcaggaa gaataaggtt caagactaat agggcaggcg
     4321 gcattttagg cgggatatcc aatggtgaag aaataagagc gagagtagct gtaaaaccaa
     4381 cccctacaat aatgaaggaa caggaaacag tagatgttga catgcagaag aatataaatt
     4441 ataagtttac atcccggagt gatccatcca tttgtccgag aatatggccg gtttgtgaag
     4501 ctatggtgag aatcactctt ttggatgcaa tccttatggc taaagcgtat agggcaatat
     4561 cattcaatct ggatccaaag tgggacttat tataacataa gtgcaatccg ccaatcggat
     4621 tgcttcacac cgtttcaata a
//
LOCUS       DAOSDD010000303         4618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6562_length_4618_cov_4.212579, whole genome shotgun sequence.
ACCESSION   DAOSDD010000303 DAOSDD010000000
VERSION     DAOSDD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_4618_cov_4.212579"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1523)
                     /gene="thrC"
                     /locus_tag="PK733_17935"
     CDS             complement(27..1523)
                     /gene="thrC"
                     /locus_tag="PK733_17935"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ12448.1"
                     /translation="MYESTRGSFKNISSAEAVKMGIAPDGGLFVPDAIPEIADEVMSR
                     IVDMDYLERAVSILKPYLLDYSEEEIIECVNNAYSLTRFDTSEIAPVRKLDDSAYILE
                     LWHGPTCAFKDMALQLLPHLLVKAAVKTGETKEIVILVATSGDTGKAALEGFRDVKGT
                     KIIVFYPYKGVSEIQEMQMVTQEGSNVFPIAVEGNFDDAQSGVKSIFTDSMLINAIDE
                     KGYRFSSANSINWGRLVPQIVYYFSAYGDMVKNNWIKPGEMVNIAVPTGNFGNILAAF
                     YSMKMGLPVKKLICASNENNVLTDFINTGVYNRNREFKKTMSPSMDILISSNLERLLY
                     EITEHDSFRIINWMKDLSDCGIYEVDNETKSRIGNLFWAGYSSEEETLNTIKAVYDKY
                     GYLVDTHTAVALNVYKKYTANKADNVKTIIASTASPFKFNESVSRAILGSNKIKGKSE
                     FELLGILSKESGMPVPGGLRGLDKKEIKHNRVCMKNEMKLQVEKILGI"
     gene            complement(1672..3063)
                     /locus_tag="PK733_17940"
     CDS             complement(1672..3063)
                     /locus_tag="PK733_17940"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ12449.1"
                     /translation="MELKMVISDEKRILNLVLGGGGIKGIAYSGVFDAAEKRGYKWGN
                     IAGVSAGALAGSYAAAGYSAEEIIKIMDQFDFSEVDINNIPDKVPAVKRLIEIKGKLR
                     NIGIIDRLKYIEIKNKLNYIEKNWMRGQYEKDFILSLLFDTEGLKTPGACRYSSEGNE
                     QGYMSDMGDLELSGEDQYRAGILNNIISYCKNGCLFDGDYLEEWVHRVLAEKGVKTFA
                     DLRGGIIDSKNPNGYRMRMTAVDSNRSKVIVLPDDMAFYGIDPDKFEVAKAVRMSTSI
                     PFVFKPVEIKKTEKDKTKVYNIIDGGVLDKFPGWLIESSNSDKQLVGFTLDGGKKKSL
                     LSVNTPLNILKWLISSVYDIGVPKDSESNIKYIGKIDTSKVYYLDFNISKEDKLYLYE
                     SGKMAAENVFNDLESSINEKYDQNNKDNINNPSKDYSQNRSYEHKKCLSVAILLLVLE
                     LSIKMKHNNLSQK"
     gene            complement(3192..4520)
                     /locus_tag="PK733_17945"
     CDS             complement(3192..4520)
                     /locus_tag="PK733_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ12450.1"
                     /translation="MIELKNELKNFTPINLENITKINPEISEDLKNSIMLYNSALANL
                     ATNSEDIAIIELKKAVAVNPDFYEAVNLLGLCFYYMKAYEKAEEMFERVARAENNGIK
                     AASYLEIIRNKDTLEDLQHEKQPPKGRTRKKVSKIASGTVPGTGYRTRTGGRSEVGLK
                     PSNTPVTTDRATSSATSSTTSSTTLDASNLTIANTTVSRRKTVSGPASLPEFPLIGRR
                     KTGSRDGAIKYLTGIIIGLIIAFIVGVPSIFKPQAGKEANKKDDNIASLEALVKEYEV
                     KYNQLSDKNETLKKELETVKLDADYYKSAIKLYEAEELLKVRKYEEAADILVVVKNAG
                     FNSSEKDKYERLSKEIMPRAADLVYSQAYNLFQAGKYRESLEKYSKIPDYMENYGKLD
                     IVLYYMGKCYLEIGDKENAKKEFDNVISKFPESEYAKYSKSRLDSIGNTQ"
BASE COUNT         1323 a          980 c          637 g         1678 t
ORIGIN      
        1 acatgaaaat tattgatcca aagccatcaa atgcccaata ttttctctac ctgcagcttc
       61 atttcattct tcatgcaaac tcgattatgc ttgatttctt ttttatcaag gcctctgagt
      121 cctccgggaa caggcatacc ggattctttt gagagaatac ccaacaattc aaattcgctt
      181 ttacccttta ttttattgga tcccagtata gcccgggaga cgctttcatt aaatttaaaa
      241 ggacttgctg ttgaagctat tattgtcttg acattatctg ctttatttgc cgtatatttt
      301 ttgtacacat tcaatgctac agctgtatgg gtatcaacca aataaccata tttatcgtac
      361 actgctttta tagtattaag tgtttcttct tcactgctgt aaccggccca aaataaattt
      421 cctatacgag atttggtttc attatcgact tcatatatgc cacagtctga cagatccttc
      481 atccagttaa taatacggaa agaatcatgt tctgtaattt cataaagcag acgttcaagg
      541 ttgcttgata ttaaaatgtc cattgaagga gacatggttt ttttaaattc cctatttcta
      601 ttgtacactc cagtattgat aaaatctgtc aacacattgt tctcattaga tgcacaaata
      661 agttttttaa ctggaaggcc cattttcatt gaatagaacg cagccagtat gttgccaaaa
      721 ttacctgtcg gtacagcaat attaaccatt tcgccaggtt ttatccaatt atttttaacc
      781 atatccccat aggcagaaaa atagtagaca atttgcggta caagtcttcc ccaattaata
      841 gagttagctg aagaaaacct gtatcctttt tcgtctattg cattaataag cattgaatct
      901 gtaaatattg attttacacc gctctgtgca tcgtcgaaat ttccctcaac ggcaatagga
      961 aaaacattgc tcccttcctg ggtaaccatc tgcatttcct gaatttcact tacaccttta
     1021 tatggataga atacgattat tttcgtaccc tttacatccc tgaatccctc cagagctgcc
     1081 tttcccgtat cacctgaagt tgcaaccaaa ataactattt cctttgtctc accggttttt
     1141 acagcagcct ttaccaataa atgaggtaaa agctgcagcg ccatatcttt aaatgcacag
     1201 gtggggccat gccataattc gagaatataa gcactatcat ccagcttgcg aacaggagct
     1261 atctcggaag tatcgaacct ggtcaagctg taagcattat ttacacattc aataatttcc
     1321 tcttcagaat aatccaataa atatggttta agtattgaaa cagccctctc cagataatcc
     1381 atatctacta tcctgctcat aacttcatca gctatttcag gtattgcgtc gggcacgaac
     1441 agtcctccat cgggagctat acccattttc acagcctcgg cagatgagat gtttttaaaa
     1501 ctacctcttg tactttcata catattataa ttcctccgta cattaatatc gttatcaaat
     1561 ataataatat aaacagaggc aaaatggaag tatattttac ggtagagagt gcatttatca
     1621 aatctgaaga aatatttacc aggagtaaga gtcgattttc atgtataata atcatttctg
     1681 cgacagattg ttatgtttca tctttatgga taactccaac acaagtaata atatggccac
     1741 tgacaagcat tttttatgtt catagctgcg attttgactg taatctttac tcggattatt
     1801 aatattatct ttattatttt gatcatattt ttcattgatt gaactttcca aatcattaaa
     1861 aacattttca gctgccattt ttccggattc atataaatat agtttatctt ctttacttat
     1921 attaaaatca agataatata ctttagatgt atctattttg ccaatatatt tgatattgct
     1981 ctctgaatct tttggcacac ctatgtcata aacagatgat ataagccatt ttaatatatt
     2041 taatggggtg ttaacgctca ataagctctt ttttttacct ccatctaatg taaaccctac
     2101 tagctgtttg tccgaattgg agctttcaat aagccagccg ggaaacttat ccagtactcc
     2161 cccatcaatt atattgtata cttttgtctt atccttttca gttttcttta tttcaacagg
     2221 cttaaagaca aatggtatac ttgtactcat tcttacagct tttgcaactt cgaatttatc
     2281 aggatctatt ccataaaaag ccatgtcgtc aggaagcaca ataacttttg acctattact
     2341 atctactgct gtcatacgca ttctgtaacc attgggattt ttagaatcta ttattccacc
     2401 cctcaaatct gcaaaagtct ttactccctt ttctgccagc actctatgca cccattcttc
     2461 aaggtaatcc ccatcaaaaa gacatccatt tttacagtaa gatattatat tgttaagaat
     2521 acctgcacgg tattgatctt cgcccgataa ctccaaatct cccatatcag acatataacc
     2581 ttgttcgttc ccttctgagg agtatcggca tgcgcctggt gtttttaagc cttctgtatc
     2641 aaataataga ctgagtataa aatctttttc atattgtccc cgcatccaat ttttctcaat
     2701 ataatttaac ttattcttta tctcaatata tttcagtcta tctattatcc ctatatttct
     2761 taacttcccc tttatctcaa tcagcctttt tactgccggt accttgtcag ggatattatt
     2821 tatatcaact tcagaaaagt caaattgatc cataatttta attatttctt ctgcactata
     2881 tcctgcagct gcataagaac ctgcaagggc tcctgctgaa actcctgcaa tattccccca
     2941 tttatagcct cttttctcag cagcatcaaa gactccacta tatgcaattc ccttaattcc
     3001 gccaccgccg agaacaaggt ttagaattct tttttcgtct gaaataacca ttttaagctc
     3061 cattcctata aaactaacat cttctttata tataatatta aaaaggcaac cctcttgtga
     3121 agggctgccc taaaatcggg ttaaaataaa ctatacagtt ttatgtatat taatatgact
     3181 ccattgttca gctactgtgt atttcctatg ctatcaagcc tgctttttga atacttagca
     3241 tattcacttt cagggaattt actaattaca ttatcaaatt ctttctttgc attttcctta
     3301 tcacctattt caaggtaaca tttacccatg tagtataata ctatatccaa tttaccatag
     3361 ttttccatat aatcaggtat ttttgagtac ttttccaggg attccctgta ctttcctgcc
     3421 tggaataaat tatatgcctg actgtaaacc agatctgcag ctcttggcat tatttccttt
     3481 gacaaccttt catatttatc tttctcagat gaattaaacc ctgcattttt gacaactaca
     3541 agaatatctg ccgcttcctc atactttctt acctttaata attcttccgc ttcataaagc
     3601 tttattgccg atttataata atctgcatcc aacttgactg tttccaattc ttttttaaga
     3661 gtttcatttt tatcacttaa ctggttatac ttaacttcat actccttaac caacgcttca
     3721 aggcttgcaa tattatcatc ttttttattt gcttccttac ctgcctgggg tttaaaaata
     3781 ctcggcaccc ctacgataaa agctataata agtccaatta ttattcctgt taagtattta
     3841 attgccccat ctctactgcc ggtttttctt ctcccaatga gaggaaattc tggtaagctt
     3901 gcaggacctg atactgtttt tcttctactt acagtagtat tagctatagt taaatttgaa
     3961 gcgtccaatg tagttgacga tgtagttgac gatgtggctg acgatgtagc tctatcggta
     4021 gttacaggcg tatttgaagg ctttagtcca acctctgacc tgccacccgt tctggttctg
     4081 tatccagttc ccggcactgt tcctgatgca atctttgata ctttctttct tgttctgcct
     4141 tttgggggct gcttttcatg ctgtaaatct tctaaagtgt ccttattcct tataatttca
     4201 agatagctgg cagcttttat cccattattt tcagctcttg ctactctttc aaacatttct
     4261 tccgccttct cataagcctt catataataa aaacaaaggc caagaaggtt aacagcttca
     4321 taaaaatcag ggtttactgc tacagctttt ttcaactcta taattgcaat atcctcacta
     4381 ttcgttgcaa ggtttgccaa ggcactgttg tacagcatta ttgagttctt caaatcttca
     4441 gatatttccg gattgatttt agtaatattt tcgagattaa taggtgtaaa gtttttgagt
     4501 tcattcttaa gctcaatcaa aaagtatcac cacccttttt atatctattt atctatatta
     4561 ataattttaa ttaaaattaa tcacgatatt taaagcatat agtttatata gttaattt
//
LOCUS       DAOSDD010000304         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6866_length_4467_cov_4.652992, whole genome shotgun sequence.
ACCESSION   DAOSDD010000304 DAOSDD010000000
VERSION     DAOSDD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6866_length_4467_cov_4.652992"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK733_17950"
     CDS             complement(<1..280)
                     /locus_tag="PK733_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12451.1"
                     /translation="MPVKDNQANLHKDIKLEMDTIIAEQQLEASAAKEYAEKGIKIEM
                     PFTDMLGIHQEFDKGHSRIERRTYYVLNDASCVLKDEWPDVAALAWLSG"
     gene            739..1968
                     /locus_tag="PK733_17955"
     CDS             739..1968
                     /locus_tag="PK733_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ12452.1"
                     /translation="MKQISARELCSQLEQELVGRHYTQDSLYRYRKVLKEFMDFTGDV
                     DFSTNLTSDFIQFIMKRDSGFSEKGEHSKKHLYYVRTMRKLEDYLLFGTFFRTKKAMP
                     PDSWPEGFSQGLDSFFTEYRDGELSELQYRRIRKNVQRFILYLAGNGFRSFTEVEYPQ
                     LTGYISSLVGYAPVTIASDISCLRVVFRYLYLNQFIHTPLHDLLPKVHNVWRTKIPNI
                     WSEEELARIKAAIDLGNPVGKRDFAIIMLAAGTGLRAGDIASLKLQDIDWEKKELSVV
                     QNKTRGFLKLPLMDDVGWAIISYLKDARPETDSPYLFVRLNFPFAPFVSSPQALYRII
                     TGYIGKAGIKTEGRSKIGIHSLRHSVANTLLNHNVGITTIADILGHSSPETTLHYLRS
                     SIEALSRCPLEVMSSED"
     gene            1958..2947
                     /locus_tag="PK733_17960"
     CDS             1958..2947
                     /locus_tag="PK733_17960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12453.1"
                     /translation="MKTKNEFHGKFADEFRLFLQEKHSLGIKYIDEERMLYQIDGMTM
                     DYDCQYGLPKALVEEFIRFRPHWAKATQESHINLIRQIALFLIKMGLPSYYPDSCWKG
                     NSTRDFHPYIYTNEEIERLFEVADNPRNRISERSRDFHSVIYRLLYSSGLRVSEALNL
                     TMGNIDFTEGTIYIRNSKNHKDRKLPIDKGMLVWMEDYASKYHKTYRPEDYFFRSPEG
                     GAYSKHTVYAYFRKMLFACGISHGGRKKGGPRLHDVRHAFCVHSLEKMLSQGTPYDAA
                     LPVLCAYMGHSSLSATTKYLHLTQELYPDISQRLEITVGSIIPVMEGIKNENE"
     gene            2934..3944
                     /locus_tag="PK733_17965"
     CDS             2934..3944
                     /locus_tag="PK733_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ12454.1"
                     /translation="MKTNDKGFPYYVSTFLTSYLAGQRNHSTNTIRSYRDTFVLLLQY
                     LSDNKNISPNRVNINTVTKETVKDFLKWLEEERGCSISTRNQRLAAIHAFFRYIQSEN
                     PEYMFQCQQILSISSKKTCHKLPVYLEVDEMKMLLEQPKLALRSGRRDAALLSLLYDS
                     GARVQELADLTPRSLRLTEPACVTLVGKGRKTRCVPLMNNTVKLLELYIQENHLESME
                     NLDHPLFFNCHGKKLTRQGISYIIDKYVNSMDISGKITPHHFRHSKAMHLTQADVNPV
                     YIRDILGHVDIQTTSIYSKADLNMKRKALEKIGDNVSPKVPDWTTDNNLLDFLKTIGT
                     SK"
     gene            4323..4467
                     /locus_tag="PK733_17970"
                     /pseudo
     CDS             4323..4467
                     /locus_tag="PK733_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         1362 a          946 c          936 g         1223 t
ORIGIN      
        1 tccctgacag ccatgccaac gcagctacat ccggccattc atcttttaat acacaagagg
       61 cgtcatttaa gacataatag gttctacgct ctatccggga gtgaccttta tcaaattcct
      121 gatgaattcc taacatatca gtgaacggca tttcgatttt tattcctttt tccgcgtatt
      181 ctttggctgc cgatgcttca agttgctgtt cggcaataat cgtgtccatc tccaacttga
      241 tatctttgtg caagttagcc tgattatcct ttaccggcaa gacaaagtcc acgcctttgc
      301 caagaataat tttggtaata tccttttgac atccgatggc ttatataaag ttattcgtta
      361 tgcaaagttg gatgttaaaa tccgcatgaa tgcacacaaa tttgtccttc caactttgca
      421 taatatttta ctcactgcaa gctttttaaa aaatccatca tatctttctt ctctttatcg
      481 tcgtaatatt cagtgtcaag gctaaccaga ttcgaagctt ttttaatagc ttttcgaata
      541 acttcgggat tgtttcggca atatacttta tgttaagctt aacataaaat ctattatgac
      601 aagcaagtaa ttatgtaaag tcatcgtcta aatccctgat attcatctca atatacattg
      661 catttcttta cataatcaca tctaatttaa ttggagcaga cgaaatctgc tactaataaa
      721 ttagatgagg tggttttttt gaaacaaatt tcagcaagag aattgtgcag ccaattagag
      781 caggagcttg ttggtcggca ctatacccag gattcgctgt accggtaccg taaggtattg
      841 aaggaattca tggattttac aggtgatgta gatttctcca caaatcttac cagcgacttc
      901 attcagttta tcatgaagcg ggatagcggc ttttcagaaa agggggaaca ttccaaaaaa
      961 catctctatt atgtaaggac tatgcgcaaa ctggaagatt atctgctgtt tgggactttt
     1021 ttcaggacaa agaaagcaat gccgccagac tcctggccgg aaggattcag ccagggactg
     1081 gattcatttt tcaccgaata cagggatggg gagctttcag aactacaata cagacggatc
     1141 agaaaaaatg ttcaacggtt cattctgtat cttgcaggca atggtttcag atctttcaca
     1201 gaggtagaat acccgcagct tacaggatac atctcttctc tggtggggta tgcccctgta
     1261 accatcgcat cggacatctc ctgtctccgt gtggtattcc ggtatctcta cctcaaccag
     1321 tttatccata ccccgcttca cgatttgctc cccaaagtcc ataatgtatg gaggacaaaa
     1381 ataccgaaca tatggagcga agaggaactc gcccgtataa aagctgcaat tgacttgggg
     1441 aatccggttg ggaaaagaga ctttgccatt atcatgcttg ctgctggaac gggactgcgc
     1501 gccggagata tagcttcttt aaaactccag gatatcgact gggaaaagaa agaattatct
     1561 gtagtgcaga acaaaacaag aggctttctg aagctgcctc tgatggatga tgtgggctgg
     1621 gcgatcatca gctacttaaa ggatgcaaga ccggaaacgg attcccccta tctgtttgtc
     1681 agattgaact ttccatttgc accgtttgtc tcatctccac aggcattata caggatcata
     1741 acaggatata ttgggaaagc aggaattaag acggaaggaa ggtcaaagat agggatccat
     1801 tcattgcgcc acagcgtggc caacactctt ctgaaccata atgtcggaat tacgaccatc
     1861 gctgatatac tgggacattc cagcccagag acaaccttgc attacttgag gagcagcata
     1921 gaagcactaa gcagatgccc tctggaggtg atgtccagtg aagactaaaa acgagttcca
     1981 tggaaagttt gccgatgagt tcagactgtt cctgcaagaa aagcattctc tcggaatcaa
     2041 atatattgat gaagaaagga tgctgtacca gattgatgga atgactatgg attatgactg
     2101 ccaatacggc cttcccaaag cactggtcga agaatttata cggttccgtc cgcattgggc
     2161 aaaagcaacc caggaaagtc acatcaacct tatccgccag atagccctgt tcctgataaa
     2221 aatggggctg ccttcttact accctgattc atgttggaaa ggaaattcca caagagattt
     2281 ccatccttac atatatacca acgaagagat agaacgactt tttgaagtgg ccgacaatcc
     2341 tagaaaccgc atatcggaaa ggagcaggga tttccattcc gtgatatacc gtttgctata
     2401 tagcagtggt ctacgggttt cagaagcttt aaatctcacc atgggtaaca ttgattttac
     2461 agaaggtaca atctatatcc gcaacagcaa aaaccataaa gacaggaaac ttcctattga
     2521 taaggggatg cttgtatgga tggaggatta cgcatcaaag taccacaaaa catacaggcc
     2581 agaggattac tttttccgtt cccctgaagg cggtgcctac tccaagcaca ctgtctatgc
     2641 atatttcaga aagatgctct ttgcctgtgg catttcacat gggggcagaa aaaaaggggg
     2701 accaaggctc catgatgtcc gccatgcatt ttgtgtccat agtctggaaa agatgctatc
     2761 ccaaggcact ccctatgatg ctgcgcttcc tgtgttatgc gcatatatgg ggcactcttc
     2821 cttatcagca accacaaaat atttgcacct gacacaggaa ctctatcccg atatatctca
     2881 aaggctggaa ataactgtgg gcagtattat tcctgtcatg gagggcatta aaaatgaaaa
     2941 cgaatgacaa aggattcccc tactatgtca gtacttttct gacttcctat ctggcagggc
     3001 aaaggaatca cagcacaaac acgatccgtt cgtaccgcga cacctttgtt ctgctgctcc
     3061 agtatttaag tgacaataaa aatatatcac caaatagagt aaatataaat acggtaacga
     3121 aagaaacggt taaagatttc ttgaaatggc tggaagaaga acggggatgc agcatatcca
     3181 caagaaacca gcgactggct gcaatacacg cattttttcg gtacatccag tcagaaaatc
     3241 cagagtatat gttccaatgt cagcagatac tttccataag ctctaaaaaa acgtgtcata
     3301 aacttccggt atatttggaa gtggatgaaa tgaagatgct tctggaacag ccgaaactgg
     3361 ctctgagatc gggaaggcgt gatgcagcat tgctgtcctt gctctacgat tccggggcac
     3421 gtgtacagga acttgcagac cttacaccgc gtagcctaag gctaacagag ccagcgtgtg
     3481 ttacactggt tggaaaagga agaaaaacaa ggtgtgtccc tctaatgaac aatacagtaa
     3541 agcttctgga attgtatatt caggaaaacc acctggaatc catggaaaat ttagatcatc
     3601 cattattctt taactgccat gggaagaagc taactcggca gggcatttca tatattattg
     3661 ataaatacgt taatagcatg gatatatctg gtaaaataac cccgcaccat tttagacact
     3721 ccaaagcaat gcaccttaca caggcagatg ttaaccctgt atacatcaga gatatcctcg
     3781 ggcatgtgga tattcaaacc acatccattt actcaaaagc tgatttaaat atgaagcgta
     3841 aggctttaga aaaaatcggt gataatgtga gtccaaaagt tccagactgg accacagaca
     3901 ataatttatt ggacttctta aaaacgatag gaacatccaa ataaattatg taaagacttt
     3961 ttcaaaaaaa tcacggctga ttcctgtgaa tacgtaggaa tcagctattt cttaacataa
     4021 tgtttttctt gtcataatag tgcttatgtt aagcttaaca taaagtctac gccagggcgg
     4081 atactgagac gcggagaaaa gcactggagg cagcatatgt cgacatcctg ccaacaagtg
     4141 aactgccgga ttggtcggat gataaaaatc tgatggattt cctgatctcg cttggaaggc
     4201 aatgataaaa tggtgagtgg tttaattggc aacctactta tttggctagt ttgctgtcgt
     4261 cactcaccat tttatatctc tcaccataat ggcgcaaatg gggagctccc catttccgtc
     4321 attcgagatc aatgcagctt tatcgtgacg gaatgccaat ggtgcttttg tcggaatatc
     4381 tcggtcattc aagtcctgag tctacgaaag tatatgcatt tgcagatacc gaaatgaaac
     4441 gtcaggcaat ccaaagagct gatgtat
//
LOCUS       DAOSDD010000305         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6876_length_4459_cov_4.896912, whole genome shotgun sequence.
ACCESSION   DAOSDD010000305 DAOSDD010000000
VERSION     DAOSDD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_4459_cov_4.896912"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK733_17975"
     CDS             <1..466
                     /locus_tag="PK733_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12455.1"
                     /translation="KEKISDETFEKIIIKYPKVKAIQNCINDFREIFANKDVTLLHEF
                     ISKYKISNMKSIAGFAGGLIKDINAVENSVTSEHSNGYVEGNNNKLKLIKRQMYGRAK
                     LPLLKAKILIRNDIFCLKSTKIEEEPGCVRNMQVLKPIGDDMYKLSDIDVKA"
     gene            534..1121
                     /locus_tag="PK733_17980"
     CDS             534..1121
                     /locus_tag="PK733_17980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ12456.1"
                     /translation="MAQHRDFALVQGWLSGNSSAGEKLITESYQKVSRLVAVKINSRT
                     NNYQETVEEITEEAFTRAFERIQTYNGACSFYTWVCGFAKNCVKERLREIYRDLKEPE
                     YKDEVDYFAEYELDDFDNPEEAACKKEEYEAIRSAFLSLQPDYREILHFRLIKGLKYD
                     AISDFSGKSIDNLESLFRRALKALKKQFYNFYKNI"
     gene            1183..1530
                     /locus_tag="PK733_17985"
     CDS             1183..1530
                     /locus_tag="PK733_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12457.1"
                     /translation="MKKIDSLLTKYVDSINSGVRIDVNEILKECPDEDREELKELIAM
                     INAFKKSYTPFEVRPQKVKKLFTTLESLRLKKQADNQKTVVNFRKDNISSDEEIKLNN
                     KLKKLFDEEFGEG"
     gene            1532..2359
                     /locus_tag="PK733_17990"
     CDS             1532..2359
                     /locus_tag="PK733_17990"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOJ12458.1"
                     /translation="MGKFSLEQGLSPEWYAKSLLNKLNIKAPPIIAKDVARELGIKYR
                     EDDLENEKVDGALYRRGDKTLIAINSQIKYEERKNFSGAHEIGHFIIESHNAESYSCS
                     KSDMEIYGSIKKVVEAEANRFAAALLMPDVFVVKAIKGETFSFDAIQRITETFKVSLS
                     AAALRFVEFTDEKCAVVFSQNGEINWSQSSKSFRYSLRRGKLLDITYAYDAFHGNDPL
                     SEKFEKVYPHAWIADADVPNDLNVQEFSCYFKNINSVLSLVKVEEDEDDDWVDEDQY"
     gene            2597..2941
                     /locus_tag="PK733_17995"
     CDS             2597..2941
                     /locus_tag="PK733_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12459.1"
                     /translation="MNHEKNNQITITVITTDGNHKDDYNVHNTLQKVVDKTIKELELT
                     INQTEYELVKKGSTNVLDLSLTIKAAGLIDDDILDYRIKNGGGGNLGEEERGVPPPSS
                     LRNLSIIYILAG"
     gene            2946..3380
                     /locus_tag="PK733_18000"
     CDS             2946..3380
                     /locus_tag="PK733_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12460.1"
                     /translation="MDEGIMRKHFEKDTEKIIKNNLLLEAREWKLVKCQYPDFLISMM
                     HPKSKKEYVFWFLCNDYPISMQVVDPISYSPVPFVQWPHGSSFLERHTLTNKPFLCIP
                     GLREYHTHDSHKNETKWIFPCNEFRLPQLLDRVYNKFINSNS"
     gene            3395..3880
                     /locus_tag="PK733_18005"
     CDS             3395..3880
                     /locus_tag="PK733_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12461.1"
                     /translation="MNNYKYFIPFNLLKETKDFLVQMGKKDKEAYVLWSGVKLSTFEF
                     QVRGMIIPEQLAVKTAFGYAFDITASSIGKINEALFDKGEMGLIQVHSHPGESAFHSP
                     RDDKLSVINRLGALSIVLPYFGNIDFSDFSKVKVHIHTGVHQWEVLPLNKVKEIIHIV
                     R"
     gene            3888..>4459
                     /locus_tag="PK733_18010"
     CDS             3888..>4459
                     /locus_tag="PK733_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12462.1"
                     /translation="MVNMEMHEENIKFLKHFGISDISEVSGKRVALVLHDKVQHSYNS
                     QHMFILSANLLSRLFLNIDFILSSGINAPMQINGNQQKLDDVVLSYAKKVFPLGNFRV
                     LDGVADFSKYDGVLIVGYGAGYGNIIFVNSNGWNIYINKAESEDRYNPIGACAAACFG
                     AAELFKIVMKPVGHRLCGIMSFSLLNYSMD"
BASE COUNT         1565 a          608 c          961 g         1325 t
ORIGIN      
        1 aaaagaaaaa ataagcgatg aaacatttga aaaaattata attaaatatc ctaaagttaa
       61 agcaatacaa aattgtatta atgattttag agaaatattc gccaataaag atgttacgtt
      121 acttcacgag tttattagca aatacaaaat atcaaatatg aaatcaattg ctggatttgc
      181 aggtgggttg ataaaagata taaatgcagt agaaaattca gtaacatctg agcatagtaa
      241 tggctatgtt gaaggtaaca ataataagtt gaaactgata aaaaggcaaa tgtatgggcg
      301 ggcaaaatta ccattgctaa aagcaaagat attaattaga aatgatattt tttgcctaaa
      361 atcaaccaaa attgaggaag aacccggatg cgtccgaaat atgcaagtat taaaaccgat
      421 tggtgatgac atgtataaac tttccgatat tgatgtcaaa gcatagatat ttgtcaaagt
      481 tgcagatgtc cgaaatagtt ttaaaaaagt gtttagtaag ggagggcgtt tgattggcac
      541 agcatagaga ttttgcttta gtgcaaggtt ggttgtctgg taacagttct gctggcgaaa
      601 agcttatcac agaaagctat cagaaggtgt cacgattagt agctgtaaaa attaattcta
      661 ggacaaataa ttatcaggaa actgtagagg aaataacaga ggaagcgttt acaagagctt
      721 ttgaaaggat tcagacatat aatggagcct gttcttttta tacatgggtc tgcgggtttg
      781 caaaaaactg tgttaaggaa agattacgtg aaatatatcg agatttaaag gaaccagaat
      841 ataaggatga agttgattat ttcgcggaat atgaactaga tgactttgac aatccggagg
      901 aagctgcctg caaaaaggaa gaatatgagg ctattagaag tgctttccta tctttgcaac
      961 ctgattatag agaaatattg cactttagat tgattaaagg attgaaatat gatgctattt
     1021 ctgatttttc aggaaagtct attgataatc tggaatctct attcagaaga gcacttaaag
     1081 ctttgaaaaa gcaattttat aatttttata aaaacatttg aaaaatgtga cggaaaaatt
     1141 ggtgaagtga caatatatat gtgaaagcga gaggggagtg ttatgaaaaa gatagatagt
     1201 cttttgacaa agtatgtgga tagcataaac tctggcgtgc gaattgacgt aaatgagatt
     1261 cttaaggagt gtcctgatga ggaccgtgag gaattaaaag aacttattgc aatgatcaat
     1321 gcatttaaga aaagttatac accttttgaa gtgagaccac aaaaagtaaa gaagcttttt
     1381 acaacacttg agagtttgcg gttaaaaaaa caggctgata atcaaaaaac agtggtgaat
     1441 tttagaaaag acaatatttc cagcgatgaa gagataaagc ttaataataa gcttaaaaag
     1501 ctatttgacg aggaatttgg tgagggatag tatgggtaaa ttttcattgg aacaggggct
     1561 gagtcctgag tggtatgcaa agagtttgct aaataaactt aacattaaag caccacctat
     1621 cattgcaaag gatgtggcta gggaacttgg aattaaatac cgggaagatg atctggaaaa
     1681 tgagaaagta gatggtgctt tatatagaag aggcgacaaa acgttgattg ctataaactc
     1741 gcagataaaa tatgaagaac ggaaaaattt cagtggagca catgagatcg gacattttat
     1801 tatagaatcc cataatgcgg agagttacag ttgttctaaa tcagatatgg agatatatgg
     1861 ttcaataaaa aaggttgtag aagctgaagc aaacaggttt gcagcggcat tattaatgcc
     1921 tgatgtattt gttgttaaag ccattaaagg agaaacattt tccttcgatg caatacagag
     1981 gatcactgaa acctttaaag tgtccttatc ggcagcagct ttgagatttg ttgagttcac
     2041 agatgagaaa tgtgcagtag tgttttcgca gaatggtgaa attaactgga gtcaaagttc
     2101 taagagtttc agatactcgc ttagaagagg aaaactactt gatataactt atgcatacga
     2161 cgcgtttcat ggaaacgacc cactttctga aaaatttgaa aaagtatacc ctcatgcatg
     2221 gatagctgac gctgatgtgc cgaatgattt aaatgtgcag gaattcagct gttattttaa
     2281 aaatataaat tcagttttgt ctttggtaaa ggttgaggaa gatgaggatg atgattgggt
     2341 agacgaggat cagtattaaa aagattattt aaaaatttta cttaaagatt ttacttaata
     2401 agagacggat cagggtaact gaatctgtct tttttattcc ttaatactaa agaagcctgc
     2461 attattaatg tggcttttta aataaaccaa gcgccaaagg agggtacagc tatggatcca
     2521 taggatatat cgaattcagg aagtccgtat tttaccagta caacattacc aaatattaaa
     2581 tgaggaggat tctattatga atcacgaaaa gaataaccaa atcacaatta cagtaattac
     2641 tactgacggc aaccacaaag atgattacaa tgtccataac actttgcaaa aggttgtgga
     2701 caagactatc aaagaattgg agctcactat taaccaaaca gaatatgagt tagtaaagaa
     2761 aggaagtacg aatgtacttg acctttcact tactattaaa gcagctgggt tgattgatga
     2821 tgatattctt gattacagga ttaaaaatgg tggtgggggc aatctggggg aagaggaaag
     2881 aggtgttccc ccaccttcct ctttgaggaa tttgtctata atctatattc tagcggggtg
     2941 acaaaatgga tgaaggtatt atgcgaaaac acttcgaaaa ggacacggag aaaattatta
     3001 aaaataattt attgttggaa gcaagagaat ggaaactggt aaaatgccag tatccagatt
     3061 ttctgatatc aatgatgcat ccaaaatcca agaaagagta tgttttttgg tttttatgca
     3121 atgattatcc aatttcaatg caggtagtcg atccaataag ctacagccct gttccttttg
     3181 tacagtggcc gcatgggtcg agctttcttg agaggcatac tctgaccaat aagccttttc
     3241 tctgtattcc gggcttaagg gagtaccata cgcatgatag tcataaaaac gaaaccaaat
     3301 ggattttccc atgcaatgaa ttccgacttc cacaacttct tgatagagtg tataacaagt
     3361 ttatcaattc taattcataa ggatggtgct tttcatgaat aattacaaat attttattcc
     3421 atttaatttg cttaaggaaa ccaaggattt ccttgtacaa atgggaaaaa aggataagga
     3481 agcttatgta ttatggagcg gcgtaaaact gagcacattt gaatttcagg tcaggggaat
     3541 gattatccct gagcaacttg ctgtaaaaac ggcatttggc tatgcttttg atattacagc
     3601 aagttcaata ggtaaaataa acgaagcatt atttgacaaa ggtgaaatgg ggcttataca
     3661 agtgcattca catccgggag aatctgcgtt tcattctcct cgcgacgata aacttagtgt
     3721 aataaataga ttgggcgcat tatctattgt tctgccttac tttgggaata tagatttttc
     3781 ggatttttca aaagtaaagg ttcatattca cactggggtg catcaatggg aagtgcttcc
     3841 gttaaacaaa gttaaggaaa ttattcatat tgtgaggtga ctgattcatg gtgaatatgg
     3901 aaatgcatga agagaatatt aaatttctga aacactttgg tatatcggat atttcagagg
     3961 tttctggaaa aagggtggct ctggttcttc atgataaagt gcaacacagt tataattcac
     4021 aacatatgtt tattcttagc gctaatttgc tttcaagatt gtttctgaat attgatttca
     4081 tattatcttc cggtattaat gctccaatgc aaataaacgg caaccagcaa aaattggatg
     4141 atgttgtgct ttcatatgcg aaaaaagtgt ttccgcttgg caattttagg gtgttggatg
     4201 gggtagctga tttttctaag tatgatggag tgctgatagt tgggtatgga gcaggctatg
     4261 gtaatatcat ttttgtaaac tcaaacggat ggaatatata tataaacaaa gctgaaagcg
     4321 aggacaggta taaccctatt ggcgcttgtg cggcagcttg ctttggagca gcagaattat
     4381 tcaaaattgt aatgaaacct gtagggcaca gactctgtgg cattatgagt ttttcattat
     4441 taaactacag tatggattt
//
LOCUS       DAOSDD010000306         4435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6919_length_4435_cov_5.398402, whole genome shotgun sequence.
ACCESSION   DAOSDD010000306 DAOSDD010000000
VERSION     DAOSDD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6919_length_4435_cov_5.398402"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK733_18015"
     CDS             <1..298
                     /locus_tag="PK733_18015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12463.1"
                     /translation="IKENLTDAQLSLQSAAETLGVSATYLSRMFSEREGMTFVDFVTY
                     AKMEYGRSLLLETELTLNEISSELNYASAQYFISRFKKHFDTTPSVYRKMHTKK"
     gene            541..2175
                     /locus_tag="PK733_18020"
     CDS             541..2175
                     /locus_tag="PK733_18020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12464.1"
                     /translation="MKKVKLTLVITLILVFMLVMSSCSTGRGSATTNTDKASTTSDTT
                     VKEITKKEAPAKFSIFYQEANQNFPEGFKHNDNWFINYICERANVELTEVNVPAYMDT
                     QTKFNLMMSSGEIPDLVERADSVAMKQYGMEGAFMPTKGIIKNSSIISPFYDDVQLQA
                     MESEDGVAYVIQIPPLNDDYNTMWVRWDLLKKMGYEKTPETLDEWVEACRSLKKYDPN
                     SMPLASRVNLEYAWFTFVPFNVGTNGCGWAYYPERGKVCNHWEGDNIVRAVRFGKMLY
                     DEDLWDKEFITTTSDEYMQKKLRNNVLLHYNNIGSLSIWIQRFINDDQKDVRLAPVMM
                     PMADGVGVDKWYHSPSVLGSYTFGINAKTKELDGIIRFLEVLYSPEVKDMAVYGREGI
                     EYKILNNEKMPILPAAADSSWCSLYGWVYVNNADTLSYKPKMMINASTALSDAEKADY
                     VDLIFNADKSVRDKIYGHLSYNPMSLALPIEDNFRNLGTQAVELQRNLIAKAIMGEIS
                     IEEFIAEKDNVVKKYQNITDAYNKSANEAKVKFNLK"
     gene            2532..3482
                     /locus_tag="PK733_18025"
     CDS             2532..3482
                     /locus_tag="PK733_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12465.1"
                     /translation="MERDLSSGENWYKGTQSFSTKFKKSIPIYMLLLPAMMLLLVFHY
                     APIFGIVIAFKNFSPFKGIIGSNWAGLKYFISFLTDENFWRVMRNTILINFYQLVFGF
                     PFPIIFAIFLNELKSDKFKKVVQTVSYLPHFVSWVVVASIVTTVLSPSSGIINSFLKS
                     VLNIEPIYFLVKQQYFRSILVVSSIWKGFGMSSVYYIASLASIDQELYQAAAIDGAAR
                     IRQTWHITLPGLRNIIIVLLVINIGSIITIGFEQIYLLYNPLVYDVGDVISTYTYRLG
                     IEKVQYSLTSAIGITQSAVNFILVYTANRLSRVIAGWSLW"
     gene            3496..4371
                     /locus_tag="PK733_18030"
     CDS             3496..4371
                     /locus_tag="PK733_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12466.1"
                     /translation="MKKTLGEKAFQYFDILIMLLILAIIIFPFMNIISLSVSGRDAIL
                     SNSVSLWPKGFNLAAYGKILGSALFLRSLINTIGITVIGTFLAVILTLMVSYALTKDF
                     LGKKFVTYYFLITMYFSGGLIPTYLVVTKYLNLRNTYWALILPYLVSVFYIIVMRSQI
                     ENIPSSIFDAAYIDGANEYQTVFQIVLPTIAPTIAAISMFFALGKWNSWFPVMIYTDY
                     NKFWTLQYYLRVVVFSKFLGAKDNTELILEEAQIPEENFRMAAIVLVALPIVSIYPFV
                     QKYFVKGIISGAVKG"
BASE COUNT         1455 a          668 c          847 g         1465 t
ORIGIN      
        1 tattaaggaa aatctgactg atgcacagct ttcattacaa tctgcagctg agacgctggg
       61 tgttagtgca acatacttaa gccgtatgtt ttctgaaaga gaaggtatga cttttgttga
      121 tttcgttaca tatgctaaaa tggagtatgg acgcagtcta ttgctggaaa cggagcttac
      181 attgaatgaa atttccagtg agcttaatta tgcctcagca cagtatttca ttagcagatt
      241 taaaaagcat tttgatacta ccccgagtgt ttaccgcaaa atgcatacta aaaaataata
      301 tgtcacaaaa atgatacagt gcaaaatatt ataacatgaa aaaatatcat aatttacgag
      361 gcttttcttt attttagtta caaattaaaa gttaataagt gcgttttttt gctgttaaag
      421 aatgaatcat tgaaatataa cttcggcatc cgtataattc tattatcaag agcttcccta
      481 aatattggaa ccatgcccat tagagaggcc ttgaaattat gttaattagg aggaatcaat
      541 atgaagaaag tcaaattaac acttgtaatt actttaatac tagtattcat gctggtgatg
      601 agttcttgtt caacaggtag gggttcagca actacgaata ctgataaggc ttcaacaact
      661 tctgatacta cagttaaaga aattacaaaa aaagaagcac ccgcgaaatt cagtatcttc
      721 taccaagaag ccaaccaaaa ctttcctgaa ggcttcaagc ataatgataa ctggttcatt
      781 aattatattt gtgaacgcgc aaatgtagaa cttactgagg tcaatgttcc tgcatatatg
      841 gatacccaga caaagtttaa cctaatgatg tcttctggtg aaatacctga tcttgtagag
      901 cgcgcagaca gtgttgcaat gaaacagtat ggtatggagg gggctttcat gccaactaaa
      961 ggcattatta aaaactcttc tataatatct cctttttacg atgatgtaca gttgcaagca
     1021 atggaatctg aagatggtgt tgcctatgtt attcaaatcc ctccacttaa tgatgactac
     1081 aatactatgt gggttagatg ggacctttta aagaaaatgg gttatgaaaa aactcctgag
     1141 actttggatg aatgggttga agcttgccgg agtcttaaaa aatatgaccc taattcaatg
     1201 ccccttgcat ctagagtaaa tttggagtat gcatggttta catttgtacc ttttaatgtt
     1261 ggcacaaatg gttgtggttg ggcatattac cctgaaagag gtaaagtatg caatcactgg
     1321 gaaggcgata acattgtacg tgctgtaaga tttggtaaaa tgctatatga tgaagacctt
     1381 tgggataagg agtttataac cactaccagt gatgagtata tgcaaaagaa actacgcaat
     1441 aacgttcttt tacattataa taacattggg agtttgagta tttggataca gcgctttatc
     1501 aacgatgacc agaaagatgt ccggttagct cctgttatga tgcccatggc agatggtgtt
     1561 ggcgttgata aatggtatca ttctccatct gttctgggat cttatacctt tggtattaat
     1621 gcaaaaacta aagaactaga tggtattatc aggttcctag aggtgcttta cagtcctgaa
     1681 gtaaaggata tggctgttta tggccgtgaa ggtattgaat ataaaatttt aaataatgag
     1741 aaaatgccaa tattaccggc tgctgctgat agctcttggt gtagtttata tggttgggtt
     1801 tatgtgaata atgcagatac attgagttat aaacccaaaa tgatgataaa tgcaagcaca
     1861 gccttgagtg atgcagaaaa agcagattac gtagatttaa tatttaatgc agacaaatct
     1921 gtcagggata aaatttacgg tcacttaagc tataatccca tgtctcttgc cttgcccata
     1981 gaagacaatt ttagaaacct gggaacacaa gctgttgaat tacagcgaaa tcttattgca
     2041 aaagctataa tgggtgaaat aagtattgaa gaatttatag cggaaaaaga taatgtagtt
     2101 aaaaaatatc agaatatcac agatgcttat aacaagagcg caaatgaagc aaaagtaaag
     2161 ttcaatttaa agtaattttt attgattaat aagccagatt atttttttga atattgctat
     2221 ataattaggc gatagatgtc cccatctcta taggtagcga gtaccacttc ggttttaaag
     2281 tgaagaatat atctcctgcc ttgttgaagc gcatggagat tagaaaattt ttctacaacc
     2341 gaaaaattta ctattgatgc gttataaaaa gatatttact acatttatag ggatttactc
     2401 gctcataatt ccaacgaaaa ccagggagct ttgtgaatcc ctttcgaaat ataaatatta
     2461 ggttgtgata tttgtaaaaa gatttctttc ttgcacatat atatcaataa aaatggaggc
     2521 aattggtgat tatggagaga gatttatcta gtggagaaaa ttggtacaag ggcacacaaa
     2581 gcttcagtac aaaatttaaa aagtcaatac ctatttatat gcttttactt ccagctatga
     2641 tgcttttact cgtttttcac tatgcaccta tattcggaat agtgattgca tttaaaaatt
     2701 tcagcccttt taaaggaatt atagggagta actgggcggg gttaaaatat tttattagct
     2761 tccttacgga tgaaaatttt tggagggtta tgagaaacac cattttgatc aatttttatc
     2821 agttagtttt tggtttccct tttcccatta tttttgcgat ctttttaaat gaacttaaat
     2881 cagataaatt taaaaaagtg gtacagactg taagctattt accacatttt gtatcttggg
     2941 tagttgttgc cagtattgta acaacagttc tttcaccatc ttcaggtata ataaatagct
     3001 tcttaaagtc agtattaaac atcgaaccaa tttatttttt ggtgaaacag caatatttta
     3061 gaagcattct ggtcgtatca agcatatgga aagggttcgg aatgtcgtcg gtatactata
     3121 tagcctcact tgcatcaatt gaccaagaac tctatcaggc tgctgctata gatggcgctg
     3181 cgcgcatcag gcagacatgg catattactc ttccagggct tcggaatatt attattgtac
     3241 ttttagttat aaacattggc agtattatta ccatcggttt tgaacagatt tatttattat
     3301 ataatccact tgtgtatgat gtcggggacg ttataagcac atacacatat cgtttaggta
     3361 tagaaaaagt acaatattca cttacctcag caatagggat tacacaatct gcagtgaact
     3421 ttattttagt ttatacagca aatagactat cacgtgttat agccgggtgg tcattgtggt
     3481 agagggaggt tgaagatgaa aaagactctt ggtgagaagg ctttccaata ttttgatatt
     3541 ttgattatgc ttttaatact tgcaataata attttcccat ttatgaatat aataagtctt
     3601 tctgtaagcg gaagggatgc aatattatca aacagtgtat cactatggcc aaaaggcttt
     3661 aatctggctg catatggcaa aatattaggc agtgcgctct tccttcgctc tcttataaat
     3721 actattggta taacagttat aggcactttt ctagcagtta ttctgacatt aatggtgtcc
     3781 tatgcattga caaaagactt cttagggaaa aaatttgtta catactattt tttaataaca
     3841 atgtatttct caggtggctt aattccaacc tatctggttg taacaaagta tttaaattta
     3901 agaaatactt attgggctct tatattacct tatttagtaa gtgtatttta tattatagta
     3961 atgcggagtc aaatagagaa tataccatca agtatattcg atgcagctta tatagatggc
     4021 gcaaatgagt atcagacagt atttcaaata gtattgccta caatagctcc aactattgct
     4081 gcaatttcta tgttctttgc tctcggtaag tggaattctt ggtttcctgt tatgatttat
     4141 acagattata ataaattttg gacacttcag tattatctga gagtagttgt gttttcaaaa
     4201 ttcttggggg caaaagataa tacggaacta atattagaag aagctcagat acctgaagaa
     4261 aactttcgta tggcagcaat tgttcttgtt gctttgccta tagtatcaat atatcctttt
     4321 gtgcaaaaat attttgttaa gggaatcata tctggtgctg tcaagggtta agtgtatttt
     4381 ataaaatgct gaaaaactgg catatagaga ggagatataa aatgaaggta ctatt
//
LOCUS       DAOSDD010000307         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6939_length_4426_cov_5.115534, whole genome shotgun sequence.
ACCESSION   DAOSDD010000307 DAOSDD010000000
VERSION     DAOSDD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6939_length_4426_cov_5.115534"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PK733_18035"
     CDS             <1..678
                     /locus_tag="PK733_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ12467.1"
                     /translation="GGKVDGCILLGTYSNDPALLELKKEGYRFCLINNYIENSGISFV
                     DVDHITGSFNAVKHLIDLGHRSIAFLNGPMLYVNSTDRLKGYRKALESYNIPYDEKLV
                     FQGNYGRKSGYYAAKKILNLKKMPSSVFAANDRMAAGLIQGLSEEGIRVPEDISILGY
                     DDSDIATLIAPQLTTMHVPFYDLGRKCAFEFIRSIRNTKEGFQIIIEPELVIRKSTDY
                     VRSPSPK"
     gene            897..1619
                     /locus_tag="PK733_18040"
     CDS             897..1619
                     /locus_tag="PK733_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOJ12468.1"
                     /translation="MKRKALIVWGGWLGHEPEQVMQIFKKTLEECSFEVEVSDTLDAF
                     LDVEKLKSLHLIVPEWTMGKITREQVQPVLEAVASGVGLAGCHGGMCDAFRESTEWQF
                     MTGGQWVSHPGGDGVEYTVNIRSSSSPIVEGIGDFVVKSEQYYVHIDPAIEVLATTRF
                     PVVNWYHASNGHADVPVVWTKRWGHGRVFYNSLGHHADVFDIKPALEIMCRGFLWAAE
                     GKDIAVQRDLNANIFESTKKMF"
     gene            1683..2786
                     /locus_tag="PK733_18045"
     CDS             1683..2786
                     /locus_tag="PK733_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12469.1"
                     /translation="MSKVKVGVIGCGNISSIYFKNGKLLEILDITACSDLDIERAKAK
                     AEEFGIPRACTVQELLSDEDIQIVLNLTIPKAHAEVTLAALESGKNVYVEKPLSIARE
                     DGLKVLKRAKSKGLLVGCAPDTFLGGGIQTCRKLIDDGWIGEPVAATAFMTCHGHESW
                     HPDPAFYYKVGGGPMLDMGPYYLTALINLIGPVKRVAGSARITFPERTITSAPKYGNK
                     VTVDTPTHIAGIMDFETGAIGTIITSFDVWSAQLPRIEIYGTEGSLSVPDPNTFGGPV
                     YIRRKDASQWSEVPLTHGFSENSRGIGLADMAYALLSSRQHRANGEMAYHVLDIMHGF
                     LDSSESGKHYDVPSTCKRPSPLPMGLSANVLDK"
     gene            complement(3097..3822)
                     /locus_tag="PK733_18050"
     CDS             complement(3097..3822)
                     /locus_tag="PK733_18050"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOJ12470.1"
                     /translation="MQVLVENGVKTKGIEIEPGVSTGVAVIEVDRSGTNHIVVIPGAN
                     GRVDMGYINRKLEYIIESDIFLFQLEIPLETVDFCVKKIRNTQQTLKQNKIIILDPAP
                     AISLTEEVLKCVDYITPNETELEALTGVKICTEEDIRKASFILLDRGVKTVIAKAGEK
                     GAFIVDRERFIHVPARKVNVVDTTAAGDSFNAGFAFGLSQNREPADCVKFANAVASLA
                     VTAKGAQEAMPNMEQVQAFINRE"
     gene            complement(3891..4187)
                     /locus_tag="PK733_18055"
     CDS             complement(3891..4187)
                     /locus_tag="PK733_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12471.1"
                     /translation="MDTDIGDDIDDALAIAFALNSPEVDLIGITTVFSDSIIEVQNEN
                     IVVETKGQYSRGTTFNKTGLNWSANNKTVDSHIKAARNVDADAFVKMFIERIIR"
BASE COUNT         1341 a          828 c          865 g         1392 t
ORIGIN      
        1 ggcggaaaag tggatggatg tatattacta ggtacatatt caaatgatcc cgctcttttg
       61 gaactaaaaa aggaaggata ccggttttgt ctcataaata actacattga aaattccgga
      121 ataagttttg tagatgtgga ccatattaca ggcagtttta atgcggttaa gcaccttatt
      181 gaccttggtc acaggagtat tgcattttta aatggtccga tgctttatgt aaacagcaca
      241 gataggctta aaggttatag aaaagcattg gaatcatata atatacctta tgatgagaag
      301 cttgtctttc aaggtaacta tggcagaaag agcggatatt acgcagcaaa aaaaatactg
      361 aatcttaaaa aaatgccttc ctccgttttt gcagctaatg acaggatggc agcaggcctc
      421 attcaaggat tgtctgaaga gggtataagg gttcctgaag atatttcaat tttaggctat
      481 gatgactcgg atatagccac cttaattgcc cctcagctta caacaatgca tgtgcctttt
      541 tatgatttag ggagaaaatg tgcttttgag tttataagaa gtataagaaa tacgaaagaa
      601 ggctttcaaa taataataga acctgaatta gttataagaa agtcgactga ttatgtccgt
      661 agcccatcac caaagtgata ggcttccaaa gcccttattg acaagactac agatagcgta
      721 caaaagaaac taaattacta aagttttgtg gtccatttat tccatgtaga atcattttta
      781 cgctatatat aattttagca gtacaagatc cacgaaaaat caatttggga aacaataaag
      841 cattcaataa ggcatgcaat gattataaaa ataactttat ataggaaggt gagtttatga
      901 aaagaaaagc tttaatagtt tggggtggat ggctaggtca tgagcccgag caagttatgc
      961 agatttttaa aaaaactctt gaagaatgta gctttgaagt ggaggtttca gatactcttg
     1021 atgctttttt ggatgttgaa aagttgaaat ccctccacct tatagtccct gaatggacta
     1081 tgggtaaaat aaccagagaa caagttcaac ctgttctgga agccgtcgct tcaggagttg
     1141 gattagccgg atgtcatggt ggtatgtgtg acgcttttcg tgaaagcacg gaatggcagt
     1201 ttatgactgg tggacaatgg gtttcacatc ccggaggaga tggtgttgaa tatactgtaa
     1261 atattagaag cagctccagt cctattgttg aaggtattgg cgattttgta gtaaagagcg
     1321 agcagtatta tgtacatatt gaccctgcaa tcgaagtttt agctactacc cgcttccctg
     1381 ttgttaactg gtaccatgcc tcaaatggac atgcagatgt ccctgtagtc tggacaaaaa
     1441 gatgggggca tggccgcgta ttctataatt ccctggggca tcatgctgat gtttttgata
     1501 ttaaacctgc acttgaaatt atgtgcagag gattcttgtg ggctgcagaa ggaaaagata
     1561 ttgcagtaca aagagatctt aacgcaaata tatttgaaag taccaagaaa atgttttgaa
     1621 cctctagttg atttacgatt attattagtg gcagaatgct ttttttaaag gagatgttat
     1681 ttttgagtaa agttaaagtt ggtgttattg gttgtgggaa tataagcagt atatacttta
     1741 aaaacggtaa acttctggaa atccttgata ttacagcatg ttctgacctt gatattgaaa
     1801 gagccaaagc taaagccgaa gaatttggca ttcccagggc atgtactgta caggaacttc
     1861 tttctgatga ggatattcaa atagtcttaa atcttaccat tcccaaagct catgcagaag
     1921 tgactcttgc tgcattggaa tccgggaaaa acgtttatgt tgaaaaacct ctgtcaattg
     1981 caagagaaga tgggctgaaa gtactgaaaa gggctaaatc taaaggactt cttgtaggat
     2041 gtgctcctga tacttttttg ggtgggggta ttcaaacctg ccgaaagctt attgatgatg
     2101 gttggattgg cgaacctgtt gccgccactg catttatgac ttgccacggg catgaaagct
     2161 ggcatcccga ccctgcgttc tattataagg ttggcggtgg tcctatgctt gatatgggac
     2221 cttattacct tacagcatta attaatctta taggtcctgt taaaagagtt gcgggttcag
     2281 caagaataac tttccctgaa agaacaataa caagtgctcc taaatacggc aataaagtta
     2341 ctgttgacac ccctactcat atcgcaggaa ttatggattt tgaaacagga gctatcggaa
     2401 caataattac gagctttgac gtatggagtg cacaactacc ccggatagag atttatggta
     2461 ctgaaggttc tctaagcgtt cctgacccaa acacctttgg aggtccggtt tatatcagga
     2521 gaaaagacgc atcccaatgg agtgaagtcc cccttactca tggattttct gaaaacagca
     2581 ggggtattgg tcttgctgat atggcttacg cgttactaag cagcagacag catagagcaa
     2641 atggtgaaat ggcataccat gtccttgaca taatgcacgg tttccttgat tcctcagaaa
     2701 gcggaaaaca ttatgatgtt ccaagcacct gcaagcgtcc ttctccgctt cctatgggat
     2761 tgtcagctaa tgtcctggat aaatgaccgg gtgcatttaa tttatttaaa gaaattattg
     2821 gtaaggagta taagtggagg atgctgtagc taggcagggg taactcttct ctcaagtaac
     2881 aggtattcct tttatgcaga agagtttccc ggtatcttaa gcattggaac agcaaaatca
     2941 gtgtttgagc aaaataacta caactattaa attattattt atcaagttat tttgtgagtt
     3001 taattttgcc cgtagctgaa tttaaaggct ctaagcacta taacggcagc ccctgataaa
     3061 tatttcttca aatccagtaa atgcgcatca taggcgtcat tccctattta taaaagcctg
     3121 tacctgttcc atattcggca ttgcttcctg tgctcctttt gcagttacag caagtgatgc
     3181 tactgcattt gcaaatttta cacagtctgc cggctccctg ttttgcgaca gtccaaaagc
     3241 aaatcctgca ttaaatgaat cacccgccgc tgtagtatct acaacattta ctttacgtgc
     3301 aggaacatgg ataaaccttt ctctatcaac aataaaagca cctttttctc ctgcttttgc
     3361 aataacggtt tttactcccc tatccagaag tatgaatgac gcttttctaa tatcttcttc
     3421 agtacaaatc tttacccctg ttaaagcttc aagctccgtt tcatttggcg taatatagtc
     3481 aacacactta aggacttcct ccgtcaagga tatagcagga gcaggatcaa gaataattat
     3541 cttgttttgt tttaatgttt gctgagtatt tctaattttt tttacgcaaa aatccacagt
     3601 ctcaagtggg atttccagtt ggaacaaaaa gatatcactc tcaatgatgt attcgagttt
     3661 gcgatttata taacccatat ctaccctgcc atttgcacca ggaataacaa caatatggtt
     3721 tgtccctgat ctatcgactt ctattactgc aactccggta gaaacgccag gttcaatttc
     3781 tataccctta gttttaacac cattttccac tagaacctgc agatattttt ttcataaaaa
     3841 tctccatgtc cccaatgtat tctccattcc tccgctgtca gctttgtttt ctaccgtata
     3901 attctttcaa taaacatctt aacaaaagca tctgcatcaa cattccttgc tgcttttatg
     3961 tgtgagtcaa cagttttatt gtttgcagac cagttcaaac ccgttttatt aaatgtagta
     4021 cctcttgaat attgaccttt tgtttccaca acaatgtttt cattttgcac ttcaatgata
     4081 ctgtcgctaa aaacagttgt tatcccgatg aggtctacct ctggtgaatt taatgcaaat
     4141 gctattgcaa gagcatcatc aatatcatca cctatgtcag tatcaattat tattttcttt
     4201 tatccatact cacataactc ccttgtttca tattaatatt tgttattttg tatgctgttt
     4261 ataaaccaat actctactaa tcggtattct attaaccagt aatttgctaa cccgtacttt
     4321 attagccaat accggattag ctaatacccg atgaacagat aaccaattag tcaataccca
     4381 ttaaccaata accgattgat tagtcaatac ttggatatac tataat
//
LOCUS       DAOSDD010000308         4418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6956_length_4418_cov_4.927802, whole genome shotgun sequence.
ACCESSION   DAOSDD010000308 DAOSDD010000000
VERSION     DAOSDD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6956_length_4418_cov_4.927802"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(714..947)
                     /locus_tag="PK733_18060"
     CDS             complement(714..947)
                     /locus_tag="PK733_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12472.1"
                     /translation="MFGSEYCESLSIILNDKQNEQAKYEVKVLQKYLIGELNPWKLQT
                     ADVDGDGNIEISLGVYKTAKFHQAYDKRPFLYN"
     gene            complement(949..1500)
                     /locus_tag="PK733_18065"
     CDS             complement(949..1500)
                     /locus_tag="PK733_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOJ12473.1"
                     /translation="MMKVYMHLAEGFEEIEALSVVDVLRRAQIETDTVSVTGKKEVTG
                     AHGIRVIADLLFEDADYSSADMIVLPGGMPGTLNLEKHIGLTEIILQFSREGKWLAAI
                     CAAPSIFGKLELLKGKSATCYPGFEKYLIDAVHSEESVVVSDKIITSKGPGTAIYFAL
                     EIVKIIKGEESAEEIREGMVVRR"
     gene            complement(1598..2185)
                     /locus_tag="PK733_18070"
     CDS             complement(1598..2185)
                     /locus_tag="PK733_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ12474.1"
                     /translation="MERFKKQLDFLIEIDKLKQVFRNTILMDASRLENDAEHMWHMAV
                     GAMLFSEYSNNKDIDMLKVLKMVLIHDVIEIYAGDTFAYDEQGHVDKEEREQNAANKI
                     FALLPREQELEFRSLWDEFEASITAEAKYAKAMDRFMPILHNYITKGAQWHKFGITSD
                     KVLAKNSIIEQGSEFLWEYVKHMVEDSIEKGYLAD"
     gene            complement(2557..3411)
                     /locus_tag="PK733_18075"
     CDS             complement(2557..3411)
                     /locus_tag="PK733_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12475.1"
                     /translation="MINKYDYSNLISLSIAPAIAAVGKQYCDLFGSIELMKDIEHRCI
                     IDGFEFQMLEEWDESTPPMDRQDRRLDVWLNSCKHSVHKIISLIKETSLRILSVHAKR
                     DIGIYLCSNKLSDVEEGKRLINEALRFASEINSPICVFHLWDTWKENFDIDFLNEVFS
                     KISLEYPNVKASIENVPTHLKGYTPYDLAKQFQWITLDLQWAALYNEFEKFIELKDRI
                     VNIHLRGELESSRWVMKDYQFDFYEVLNTIIENWNYRGLLTMEPTKLKEGHLNELITA
                     MTSLGRNG"
     gene            complement(3745..3900)
                     /locus_tag="PK733_18080"
     CDS             complement(3745..3900)
                     /locus_tag="PK733_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12476.1"
                     /translation="MIDAIKARINQGMPVFVFRIIGPPECVIVAGYKDNGNISAGYSF
                     FLRLEFK"
     gene            complement(4041..>4418)
                     /locus_tag="PK733_18085"
     CDS             complement(4041..>4418)
                     /locus_tag="PK733_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ12477.1"
                     /translation="DITPPIQPNLEYDIDPKAAWALRHLDMYPVEVNKADFDTLIRVP
                     GIGITYAKRIIEARKHCTITHDILKKMRVPLKRCIYFIICNGKYKGGNVLFQTNIRHY
                     LSTDLEQISIYNSLTADEKDSCE"
BASE COUNT         1392 a          859 c          597 g         1570 t
ORIGIN      
        1 aataataaca tgtcccccct gcggaattat accatgtaaa acaatattga gtgctgttgc
       61 agcattatca aggtaaatca tacagaaaaa cctttctcat taactactac tttgttatgt
      121 tgccaatgga aagcgcattt ctggaaggta attttgctaa cccgtacggc aaaaagtttt
      181 aaaaaaaggc aaaaaatttg ttgatatatg ccttatatta ttgtacagcg ggcatatcta
      241 ttatataata aatatataat aattttccag gggtaaaatt ttatgaggta ttcgtatatc
      301 atttttttca gataagtaag tactttatta tgtataagtg ttttacgtat aagtgtttta
      361 tctgcaatgc taactctttt acttactttg agtgcctgtg aaggacacaa cgtaaatatt
      421 acttttactt aaaatgaaaa tgggaagcta tatgtatctt accgactaaa ttcttcagaa
      481 ttttacatga tgaagaaaat cattttggtg aaaattcatt tatagtttct gttgagaatt
      541 ttaaactaca gatgaaattc cttagggata ataattatgt tacaataacc ttaaaccagt
      601 tggaaaacta tattaaaggg aatactgaac ttccgggaat ctgcaaaaat aaaatcttca
      661 aaaggcctgg acagtcttga acccagccat ttatgggata taccttcatc atgctaattg
      721 tataaaaatg gccttttatc atatgcctga tgaaacttgg ccgttttata cacaccaagt
      781 gaaatctcaa tatttccatc tccatcaaca tcagcagttt gcagcttcca ggggttcagt
      841 tcgcctatta aatatttctg caacacttta acttcatatt ttgcttgctc gttttgcttg
      901 tcatttagaa ttatcgacag actttcacaa tactcagagc caaacatttc accttctaac
      961 taccattcct tctcttatct cttctgctga ctcctcaccc ttaattattt ttacaatttc
     1021 tagagcaaag tatatagccg taccgggtcc ttttgatgtt attattttgt ccgacactac
     1081 tacagattct tctgaatgca ctgcatcaat caaatacttt tcaaatcctg ggtaacatgt
     1141 ggccgactta ccttttaaca gctcaagctt accaaatatg cttggtgcag cacaaatagc
     1201 agcaagccac ttaccttctc tggaaaattg aagaattatt tctgtaagtc ctatatgttt
     1261 ctccagattt aatgtacccg gcattcctcc cggtaatact atcatgtctg ctgaagagta
     1321 atcagcatct tcaaatagca aatcagctat aactcttatg ccatgggccc cggttacttc
     1381 cttttttcct gtcacagaaa cagtatccgt ctctatctgc gctcttctta aaacgtcaac
     1441 aaccgataat gcttcaatct cttcaaatcc ttcggctaaa tgcatataaa ctttcatcaa
     1501 gtaaacactc cttccatttt atattactta cttatattac ttaagctata aaatacaata
     1561 taactaaaac atttatattt ccaaaaattc aatcaaatca atctgctaaa tagccttttt
     1621 caatactatc ctccaccata tgcttaacat attcccatag gaattcagat ccttgttcaa
     1681 taatactgtt ttttgccagt actttatcgc ttgtaatacc gaacttatgc cattgagctc
     1741 cttttgtaat ataattatgt agtataggca taaacctgtc catggcttta gcatatttag
     1801 cttctgcagt tattgatgct tcaaattcat cccataaact tctaaattcg agttcctgtt
     1861 ctctaggtag cagagcaaag attttgtttg ctgcattttg ctccctttct tctttatcaa
     1921 catgcccctg ttcatcatag gcaaaagtat ctcctgcata aatttctata acatcatgta
     1981 ttaataccat ttttaaaact ttcaacatat caatatcttt attatttgaa tactcagaaa
     2041 agagcatagc accaactgcc atatgccaca tatgttcagc atcattttca agtctcgaag
     2101 catccatgag aatagtattt ctaaacacct gcttcaattt gtcaatttca attaaaaagt
     2161 caagctgctt tttaaatcgt tccatatcat tatcaattat tatattacta gacaagtcat
     2221 agttgttatt agagctacaa ttattataga tgttattaca catacagtta ttattaatat
     2281 tctttttttt actttcagtc atataatgct tttctccatt tcttattaac acattttgtg
     2341 gttatataaa tactactttg ttatgtaaga caaatgcaac tatataggac aggttttaaa
     2401 acatttaaaa cctgcttctg ttaccttgat gtaacttacg atttgtaaat tcttcattaa
     2461 ctctttcaaa tcgtaaatta tatagtgtaa acattataaa tatatccact aggggtgtat
     2521 ttaaacatga aacttaatat ttgtgtataa aaatcattat ccatttcggc ctagggatgt
     2581 catagctgta ataagttcat taagatgacc ctctttaagc ttggtcggtt ccatcgttag
     2641 tagtcccctg taattccaat tttctataat cgtattaaga acctcataaa aatcaaattg
     2701 gtagtctttc attacccatc ttgaactttc caattcccct cgtagatgta tatttactat
     2761 tctatcctta agttcgataa atttctcaaa ctcgttatat aaagctgccc attgcaaatc
     2821 aagagttatc cactgaaact gtttcgctaa atcataagga gtgtaacctt tcaggtgtgt
     2881 gggtacattt tctatactag ccttaacatt tggatactct aacgatattt ttgaaaagac
     2941 ttcgtttaaa aaatcaatat caaagttttc cttccaagta tcccataaat gaaatacaca
     3001 gataggtgag ttgatttcac tggcaaaacg tagcgcttca tttatgagcc gcttaccttc
     3061 ctccacatca gataatttat tagaacacaa atatatacca atatcccgtt tagcatgaac
     3121 tgataaaatc cgtaaactag tttcttttat taaagatata attttatgaa ctgaatgttt
     3181 gcaactattt aaccaaacat ccaaacgcct atcctgtcga tccatcgggg gagtgctttc
     3241 atcccactcc tctaacatct gaaactcaaa accatctatt atgcatctat gttctatatc
     3301 tttcatcaat tcaatagagc caaaaaggtc gcaatattgt ttaccaactg ctgcaatagc
     3361 aggtgcaatc gataaggata ttaaattaga ataatcatat ttatttatca tgccttcatc
     3421 tccaatttac acaaacaagg tagaaagccg caatgccttt aggctcatgg atgaatacct
     3481 taatttaaaa tggcagtttt tatcgttgta agccaagaac tgccaaactc atatataata
     3541 tcataccata aatttttccc ataaaccatt ttatatctca taatcatact attttcaatt
     3601 tggataacac atccaaggaa tatcttacct tatgcaggat taatacatat cctgcataaa
     3661 agctatcctt gatataaata tactatagtt tatttagaaa gtatgtagca taataacgta
     3721 ttaatatcat tcttagttac ctactcattt aaactctagt cttaaaaaga aactataacc
     3781 agctgatatg ttgccattat ctttatatcc agctacaatg acacactctg gcggtccgat
     3841 aattctgaaa acaaacaccg gcataccttg atttattctt gcttttatag catcaatcat
     3901 tttttgtttg tttccgtgag gtattgtatc taaccttgtt tggatattcc tatcagaatt
     3961 ttcgaattat tatcatttgt catataatgc atccttcttt tttaaatact ataatcataa
     4021 tagttaacta tcctttgatt ttattcgcat gaatcttttt catctgccgt aagcgagtta
     4081 tatatggata tttgctctaa gtctgtggac aagtaatgcc tgatgtttgt ttggaataaa
     4141 acatttccgc ccttatattt accgttacag ataataaagt agatgcaacg tttgagcgga
     4201 acccgcattt tcttcaatat gtcatgcgta atcgtacagt gctttctcgc ttcaatgatc
     4261 ctttttgcgt atgtgatacc gatacccggg acacggatca atgtatcaaa atctgctttg
     4321 tttacctcga caggatacat atcaagatgc ctgagtgccc aggctgcttt tgggtcaata
     4381 tcatattcta aattgggttg aataggcggt gttatatc
//
LOCUS       DAOSDD010000309         4413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_6973_length_4413_cov_5.597063, whole genome shotgun sequence.
ACCESSION   DAOSDD010000309 DAOSDD010000000
VERSION     DAOSDD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_4413_cov_5.597063"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..627
                     /locus_tag="PK733_18090"
     CDS             88..627
                     /locus_tag="PK733_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HOJ12478.1"
                     /translation="MNPLSNNKQSAENKLILLYIIEKLDMPVSNLQITKLIMENKFMN
                     YFFLQQFLNELCDDNLLCTEMEDSKTFYSITETGKKTLNYLSYLILPVIKEKIDNTLK
                     AAKKKIRNETLITADFIPESENEYIVNCCVREDNFTLIELKLAVGSRNDARNICDNWK
                     NNSHHIYAEIIESITKNRG"
     gene            complement(633..923)
                     /gene="yhbY"
                     /locus_tag="PK733_18095"
     CDS             complement(633..923)
                     /gene="yhbY"
                     /locus_tag="PK733_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOJ12479.1"
                     /translation="MLTGKQRSYLKGIANSIQPILQIGKGGINENVICQFDEALEARE
                     LIKATVLRNSEDDARTACEEIAGLTGAEIVQVIGSRFVLYRESKNNKVINLP"
     gene            complement(1007..1195)
                     /locus_tag="PK733_18100"
     CDS             complement(1007..1195)
                     /locus_tag="PK733_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12480.1"
                     /translation="MECFKCGNCKNGSMAYFCPMKNDFIMNEEAKSTVIEKSRSGWKK
                     GQPDYETHRRKNKKEVEV"
     gene            complement(1356..2630)
                     /gene="obgE"
                     /locus_tag="PK733_18105"
     CDS             complement(1356..2630)
                     /gene="obgE"
                     /locus_tag="PK733_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOJ12481.1"
                     /translation="MFIDRARIYIKAGDGGNGIVSFRREKYIAAGGPNGGDGGKGGDV
                     VFTVDEGLRTLIDFRYKKHYKSEPGNKGEGANRTGKSGQDLFIKVPPGTILKDETTGR
                     IIADLTKPGETFVAAKGGRGGKGNQHFATPTRQVPNFSQNGEPGEEKWINIELKLLAD
                     VGLIGYPNVGKSTLLASVSAARPKIADYHFTTITPNLGVVSLEVGNSFVLADIPGLIE
                     GAHTGAGLGHEFLKHIERTKLLIHVIDISGIEGRNPLDDFETINRELEGYNELLVEKP
                     QIIAANKIDIPAGETNLEGFKSAIEDKGYKVFPISAATGKGVRELMMYAGEMLKTIPD
                     TKLTAYEDEMVVYDIDEKEPFTVRKENNIYVVEGELVKKILGSVNLGDYESLQYFQRT
                     IQKAGIIDALKESGINEGDTVKMYDFEFDYIE"
     gene            complement(2815..3093)
                     /gene="rpmA"
                     /locus_tag="PK733_18110"
     CDS             complement(2815..3093)
                     /gene="rpmA"
                     /locus_tag="PK733_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ12482.1"
                     /translation="MIRVNLQLFAHKKGVGSSRNGRDSESKRLGVKTGDGQFVLAGNI
                     IVRQRGTKIHPGDNVGKGSDDTLFALTDGTVKFERKGRDKKKVSIMAM"
     gene            complement(3096..3425)
                     /locus_tag="PK733_18115"
     CDS             complement(3096..3425)
                     /locus_tag="PK733_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOJ12483.1"
                     /translation="MIKVKVVRDRQGFIWEFTINGHAGYKKNGEDIVCAGVSAVAYTA
                     VGALAEMAGISNFSEKDGYMKCSIPPDIDQHSKHYAKIILDAMIIGMKQIEYSYGKYV
                     VVEEEEV"
     gene            complement(3431..3745)
                     /gene="rplU"
                     /locus_tag="PK733_18120"
     CDS             complement(3431..3745)
                     /gene="rplU"
                     /locus_tag="PK733_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ12484.1"
                     /translation="MYAVVDIGGKQYKVQEGDVVFIDKIEAEEGDVLTFDKVLAVSND
                     GVVNFGKPLVAGTNVQAKVLGHGKYKKIIVFKYKPKKNYRKTQGHRQPYTKVQIDRIA
                     IS"
     gene            complement(3872..>4413)
                     /locus_tag="PK733_18125"
     CDS             complement(3872..>4413)
                     /locus_tag="PK733_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOJ12485.1"
                     /translation="SLKNKIQYFSKSKSVFEYYQIDYRLSKALTRKVWLKCGGYIVID
                     RTEALTVIDVNTGKYVGSDNLEDTVLKTNMDAAAEISRQLRLRDIGGIIIIDFIDMNN
                     DEHKKMVLNALKIALRKDRTKTTVIGMTGLGLIEMTRKKVGYELANTINDECPYCKGT
                     GKNIHEQGDGRINQNVNIV"
BASE COUNT         1256 a          981 c          597 g         1579 t
ORIGIN      
        1 ttatgcattt attgaaacgg tgtgaagcaa tccgattggc ggattgcact tatgtattag
       61 tatgtatgaa ttttaaagga ggtttatatg aatccactaa gtaataataa acaatccgca
      121 gagaataaat taattctctt atatattatt gagaaacttg atatgcctgt aagcaacttg
      181 cagataacaa aactcataat ggaaaacaag tttatgaatt acttttttct gcagcaattt
      241 ctaaatgaac tatgtgatga taatcttctt tgcactgaaa tggaagatag taaaactttt
      301 tattcaatta cagaaacggg aaagaaaact cttaattatc tttcttacct tattttgcct
      361 gttataaaag aaaaaataga taatacctta aaagcagcta aaaagaaaat caggaatgaa
      421 acccttataa cagctgactt cattcctgaa agtgaaaacg aatacatagt aaattgttgt
      481 gtacgtgaag ataattttac actaattgaa ctcaaacttg ctgtcggttc aagaaatgat
      541 gcccgcaata tatgtgataa ctggaaaaat aattcccatc acatctatgc ggaaataatt
      601 gaaagtatca caaaaaatag gggttgatcc tgctacggta agttaataac tttattattc
      661 tttgattctc tatataggac aaagcggctg ccaataacct gtactatttc ggcacctgtt
      721 aaacctgcaa tttcttcgca agcagtcctt gcatcatctt ccgaattcct gaggacagtt
      781 gcttttatta attcacgtgc ctctagtgct tcatcaaatt ggcatattac attctcatta
      841 ataccgcctt ttcctatctg aagaataggt tgaattgaat tagcgatacc tttcaaatag
      901 cttcgttgtt ttcccgttaa catttgttgg gtttttcctc cttttaatca cctgtatata
      961 ccagacattc aaattgaatc aaactggaac tttggtgaac taccgctcac acctcaactt
     1021 cctttttgtt ctttcttcta tgagtttcgt agtccggttg tccttttttc catccacttc
     1081 tacttttctc aatcactgtt gattttgcct cttcattcat aataaaatca tttttcatcg
     1141 ggcaaaaata agccatactg ccatttttac aattaccaca tttaaagcac tccataggat
     1201 taaaaaatat tccctccgtt caatttttca atactttttt caatactttt aaaatgcttt
     1261 ttattgcttt gctttaattt cattctataa cacatcctgc atttttgtct atataaatat
     1321 caaaaaatga ttgtataatc aatccataat actggctatt ctatataatc aaattcaaag
     1381 tcatacattt ttaccgtatc accttcatta attcctgatt ccttaagtgc atcaataatt
     1441 cccgcctttt gtattgtcct ctggaaatat tgaagagatt cataatcgcc cagatttact
     1501 gaaccaagta tttttttgac cagttcgcct tctacgacat atatattatt ttcttttctg
     1561 accgtaaaag gttctttctc atcaatatca tagactacca tctcatcctc atatgctgta
     1621 agttttgtgt cgggaattgt ttttagcatt tccccggcat acatcattaa ttcacgaaca
     1681 ccttttccag ttgctgcaga aatagggaat accttgtacc ccttatcctc aattgcactt
     1741 ttaaatcctt caagatttgt ttctccagca ggaatgtcta tcttgtttgc agcaattatc
     1801 tgaggctttt caaccaataa ttcgttatat ccttccagtt ccctgttaat cgtctcgaag
     1861 tcatctaaag gatttcttcc ttcaattcct gatatgtcaa ttacatgaat aagtaatttt
     1921 gttctctcta tatgtttcaa aaattcatgt ccaagccctg cacctgtatg tgcaccctct
     1981 ataagtccgg gtatgtctgc taatacaaag ctgttgccca cttccagcga tactacaccc
     2041 agattgggtg taatggtggt aaaatgataa tctgctattt taggacgtgc agcagataca
     2101 cttgcaagga gagttgactt tcctacatta ggatagccaa taagacctac atcagctaaa
     2161 agctttaatt ctatatttat ccacttttcc tctccaggct ctccattttg tgagaaattg
     2221 ggcacctgcc tggtaggagt tgcaaaatgc tgattgcctt ttcctcctct tcctcctttt
     2281 gctgccacga atgtttcccc tggctttgtc agatctgcta taattctgcc tgttgtttca
     2341 tctttcaata tagtacctgg cggtactttt ataaaaagat cctgaccgct tttgcccgta
     2401 cggtttgcgc cttctccttt attccccggt tcagatttat aatgcttttt atatctaaaa
     2461 tctatgagtg tccggaggcc ttcatcaact gtaaaaacaa catctccgcc ttttcctcca
     2521 tctccgccat tcgggcctcc cgcagctata tacttttcac gccggaatga tactatccca
     2581 tttcctccgt caccggcttt tatatatatt ctggccctgt ctataaacat attattatca
     2641 actcctgttt cattcactaa tcatggaact cattatattt attcctttgc ggaaactcgc
     2701 attttattat acacattatt ttattatata tgtaagcaat atgaaagtga aaaagttaaa
     2761 aaaataatcc cggagaatac cgggattatc atataatcaa gttcgtaatt taacttacat
     2821 agccattata ctaactttct ttttatctct accctttctt tcgaacttaa ctgttccatc
     2881 cgtaagagcg aaaagagtat catcactacc ctttcctaca ttatcacctg gatgaatctt
     2941 tgtacctctt tgccttacaa taatattgcc tgccagtaca aactgtccat cgcctgtttt
     3001 aacaccaagt cttttagact cactgtcacg accgtttctg gagctaccta ctcccttttt
     3061 atgtgcaaat agctgaagat taactctaat cataattaca cctcctcttc ttcaacaacc
     3121 acatatttcc cgtatgagta ttctatctgt ttcataccta taatcatagc atcaaggata
     3181 atctttgcgt aatgcttcga atgctgatct atatcaggtg gaatgctgca tttcatatat
     3241 ccgtccttct cgctgaaatt actgataccc gccatttcag ctaaagctcc cactgctgta
     3301 taagcaactg cagacacacc cgcacatact atatcctcac cattcttttt gtatcctgca
     3361 tgcccattga ttgtaaattc ccaaataaat ccctgtctat ctctaacaac ttttactttt
     3421 atcattgcaa ttaactaatg gcaattctat caatttgtac tttagtgtaa ggttgtctgt
     3481 gtccttgtgt ttttctgtaa tttttcttag gtttatactt aaacacaata attttcttgt
     3541 acttaccatg acccagaacc ttagcttgta cattagtgcc tgctaccagc ggttttccaa
     3601 aattcacaac tccgtcgttt gatacagcta aaaccttatc gaaagtcaaa acgtcgcctt
     3661 cttctgcttc aattttatcg ataaaaacaa cgtcgccttc ctgtacctta tactgctttc
     3721 caccaatatc aactactgca tacattacag cacacctcct cttaccagtc tcgccataaa
     3781 aaggcagcac atagtgcttt ttaaaaaagc ctttcatgag cggctaccga agtattttag
     3841 catatccccc agacaatgtc aatctatttt ttcacacaat atttacgttt tggttaattc
     3901 taccgtctcc ctgttcatgt atatttttac ctgtaccttt acaatagggg cactcatcat
     3961 ttattgtatt ggcaagctca tatcccacct tttttcttgt catctcaata agtcccaacc
     4021 ctgtcattcc tataactgtt gtcttggtac gatcttttct taatgcaatt tttaaggcgt
     4081 ttaagaccat tttcttatgt tcatcattat tcatatctat gaaatcaata ataataattc
     4141 caccaatatc tcttaatctt aattgtcttg atatttccgc agctgcatcc atgtttgttt
     4201 taagtaccgt atcctccagg ttatccgatc caacatactt ccctgtattt acatcgatta
     4261 ctgtaagtgc ctcggttcga tcaataacaa tataacctcc acactttaac catactttcc
     4321 ttgtaagagc ttttgaaagc ctgtaatcta tctggtaata ttcaaagaca cttttactct
     4381 tgctaaaata ctgtatcttg tttttcaaag aag
//
LOCUS       DAOSDD010000310         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7029_length_4388_cov_4.319409, whole genome shotgun sequence.
ACCESSION   DAOSDD010000310 DAOSDD010000000
VERSION     DAOSDD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7029_length_4388_cov_4.319409"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PK733_18130"
     CDS             <1..786
                     /locus_tag="PK733_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12486.1"
                     /translation="DLFKNIDSYKLCVVAEQNNLSKEKIEKLKDYVKNGGNLIVSGAE
                     HTKKFDDIAGVKDLGTVVGEDTGQSLYIRDGKTLFTALGYWRLVKIDDAIEVEPLMFS
                     RNLGERQKNSGSPAAIIKEYGKGKIALIFGPVFGTYFDSHYPGARRFIREIIDKLGIN
                     GLAKVEAPVCVNITIREKENRIMINFVNLGTGKALSPRNTIIEDVPPVGPTKVSIPLY
                     KEPSAVFLAPSGMPVEWEYEGNTGLLKAKIDLIGIHDILVVEK"
     gene            1649..3352
                     /locus_tag="PK733_18135"
     CDS             1649..3352
                     /locus_tag="PK733_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12487.1"
                     /translation="MKRLPYGISNYKMLVEENLFYVDKTMYIEKLENLHSYYIFFLRP
                     RRFGKSLFVSLLQHYYDINEKDNFEALFKDTYIGQNPTKERNSYYVLKFNFSGITTDT
                     KEQLEESFTTITRNAFGLFQDRYNIHFDYLESGSPASIFEDFIAKITTKIDKPVYVLI
                     DEYDHFANELLSFQFNLFADSISKTGFVRKWYEVLKKATESIVKRIFATGVSPITLDS
                     LTSGFNIADNVTREEDFNEMMGFTENEVRQMVKETARFPMDENDMDDLMEKLHKNYNG
                     YLFSEKAKERLFNSDMILYYLKTYIEDKEGPIDLIDQNIASDYGKLGKTFDLKGKKSN
                     MEVLEKILNGEEIVAQITRQFSMEKDFTTDDFKSLLFYLGLLTIDKRILSRVKLKVPN
                     YVIKDLYFGYFEKKVREAINYEIDTVEISDAVSQIALEGKNNQFISVIENTLHKLSNR
                     DYISFDEKYIKLIMLSYFMLSKMYMVKSEYEVESGYIDIALLRREPIDPDYFAIFEVK
                     YIKKSNYEAYGEKIVEEKKNEAIAQILRYNTSNELKNLSNLKKWVIVFAGDKCFANVE
                     I"
     gene            complement(3592..>4388)
                     /locus_tag="PK733_18140"
     CDS             complement(3592..>4388)
                     /locus_tag="PK733_18140"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HOJ12488.1"
                     /translation="AINYVNRKELQPYWVNTASNDLIIECLRKSDNIFKDKFSKLLNY
                     EAIKVKIEKEMTYEMLDMEDNIWGLFMNAGYITLAEGNTGFIDYNIKIPNHEIQLEFE
                     KIITISANMKSSYLYDMFQALLENNIELFVYRYKLLLMQVMSYHDNRENAYHMFMLGL
                     CATLRNIYTVKSNLEVGLGRSYIILIPKDKRYHPIIMEFKATGKEDAEKAEINEEKLE
                     TLVDEAIKQIKDKKYYKVFEGQGFKDILLMGIGHHKKQCVVKTEFI"
BASE COUNT         1536 a          621 c          811 g         1420 t
ORIGIN      
        1 gacttgttta aaaatataga tagctacaag ctctgtgtag tagcagaaca gaataatctc
       61 tctaaagaaa agattgaaaa gctaaaagat tatgttaaaa atggtggtaa cttaatagta
      121 tctggtgcag aacacacgaa aaaatttgat gatatagcag gagtaaaaga tcttggaacg
      181 gttgtgggag aagatacagg gcaatcatta tatatcaggg atggaaagac acttttcaca
      241 gcactgggtt actggagact cgtaaaaatt gatgatgcta ttgaagttga accactgatg
      301 ttttctagaa atcttggcga aagacagaaa aactcaggtt ctccagctgc aattattaaa
      361 gaatatggaa aaggcaagat cgctcttatt tttggtccgg ttttcggtac atattttgat
      421 tcccactatc cgggtgcaag acggtttatc agagaaataa ttgataaact tggaataaat
      481 gggctggcta aagttgaagc accggtttgt gtaaatataa ctatcaggga aaaagaaaac
      541 agaattatga ttaattttgt taatcttggg accggaaaag cactgtctcc aagaaacacg
      601 attatagagg atgttccacc tgttggacct actaaagtgt caataccttt atataaagaa
      661 ccttcagcag tatttcttgc accctcaggc atgccggttg aatgggagta tgagggcaac
      721 acaggtttac ttaaagcaaa gattgattta ataggtattc atgatatact tgttgtggag
      781 aaatgacagt atagggttta tgtccctttc taatatgtct ggcatcagta ctgcaatggg
      841 acataaacga cttatatttc aaagcagtaa ataatgtaaa tataaggaaa tctttcctga
      901 agtactaaaa gaaatgagct tcgatgctca atataaggct gatgatgtaa gtggtgaaaa
      961 agtagaggtg cctggctctg aacagtaatg attggtaaaa ggttaaggta aaaagataaa
     1021 tgtataaata ataatcataa gtggcatcag aaggccgaaa tgaaagcggt tttctgatgc
     1081 tggtcttatt ttcaaccgaa actgcataag gatgaattac agaatatgga taagcaattt
     1141 accgtttcac atattaggag aaagccgtga gaaatttgaa tatcaataag cttataggct
     1201 ctcctgttat acattcatgt atctactatc atattcgact ggaatctatt ttctacggaa
     1261 aagacaaccc tgaaaagaat gatgaactac atacctttat gtacacagct tgcaccttta
     1321 tatgtaaagc gaaattttga agagcgtaaa aagaatggcg tggttataga aggtacctag
     1381 atatccctag cttacaccag ccagatttgc aaactgactt aatgtaatat ttgtattgtg
     1441 aagcattact ctgtcaagta cttttaccag ttggggtgca tttaatttct tcaattccag
     1501 taaatgcact cagaataaag atataaattg tactaatcag ggggaatagg atataatatt
     1561 attattatat gctttttatt atgtgcaaat agacagatta aatctataat cgaatgaatt
     1621 ttgaaaagtt tgaaagggga gagttaagtt gaaaaggctt ccatacggta taagtaacta
     1681 taagatgcta gtagaagaaa atttatttta tgtcgataag actatgtata ttgaaaaatt
     1741 agaaaacctg cattcatact atatattttt cctacgacct agaaggtttg gtaaaagctt
     1801 atttgtgtcg ctgctccagc attattacga tataaatgaa aaggataatt ttgaggcttt
     1861 atttaaagat acatatatag ggcaaaatcc aacaaaagag aggaatagct actatgtttt
     1921 gaaattcaat ttttctggta ttactactga tacaaaggaa cagttagaag aaagttttac
     1981 tactattact agaaatgcat ttggcttatt tcaagataga tataatatac attttgatta
     2041 tttggaatca ggttctccag ctagtatttt tgaagatttt atagcaaaaa tcaccacaaa
     2101 aattgataaa ccagtttatg tgctaattga tgagtatgac cactttgcta atgaactttt
     2161 aagcttccaa tttaatttgt ttgcagattc aataagcaaa accggttttg taagaaaatg
     2221 gtatgaagta ttaaaaaaag ctactgaatc aattgtaaaa cgaatatttg ctactggtgt
     2281 aagcccaatt accttggata gccttaccag tgggtttaat attgctgata acgtaactcg
     2341 ggaagaagac ttcaatgaaa tgatgggttt tactgagaac gaagtgaggc agatggttaa
     2401 ggaaacagca cgcttcccaa tggatgaaaa tgatatggat gatttgatgg agaaacttca
     2461 taagaattat aacgggtatt tgttttcaga aaaagcaaaa gagagattgt ttaacagtga
     2521 catgattttg tattatttaa aaacatatat agaggacaaa gagggaccta tagatttaat
     2581 agatcagaac atagccagcg attatggaaa actaggtaaa acgttcgacc tgaaaggtaa
     2641 gaagagcaat atggaggtcc tggaaaaaat attaaacggg gaggaaattg tcgcacaaat
     2701 aaccaggcag ttcagtatgg aaaaggattt tacaactgat gatttcaagt cattgctgtt
     2761 ttaccttgga cttcttacca tagataaaag gatactatcc cgtgtaaaat taaaagtacc
     2821 gaattatgtt atcaaagatc tatattttgg atattttgaa aagaaagtaa gagaagctat
     2881 aaattacgaa attgatactg tagaaataag tgatgctgta tctcaaattg cacttgaagg
     2941 caaaaataat caatttatat cagttattga aaatacattg cacaagcttt ccaaccggga
     3001 ttacattagt tttgatgaaa aatacataaa gctaataatg ctttcatatt ttatgttaag
     3061 taaaatgtat atggtaaagt cagagtatga ggtagaaagt ggatatatag atattgcatt
     3121 attaaggaga gaaccaatag accctgatta tttcgcaata ttcgaggtta agtatatcaa
     3181 aaaatcgaat tatgaagcat atggagaaaa aattgttgag gaaaagaaaa atgaagccat
     3241 agcacaaatt ttgagatata atacatccaa tgagcttaaa aacttatcta atcttaaaaa
     3301 gtgggtaatt gtatttgcag gagataaatg ttttgctaat gttgagatct gatttttcct
     3361 gaaaaactaa ttgggcttgt aaacatcaaa gaccagaaat caattataag gaagctaagt
     3421 gtattaatgg ttataagaga aattttttac cggagttttt tagaatattc ttaattatga
     3481 gagcaaatta cttgtagcct gttgccagag tattctaaac tactgactac tggcagctat
     3541 gctgcggtat gctatgcatt gtagtgcaag ttgtacatgg cactttgttt attatataaa
     3601 ctcggtttta acaacacatt gtttcttatg atgacctatt cccataagta ggatatcttt
     3661 gaagccttgt ccttcaaata ccttataata ttttttatct ttaatttgtt ttattgcttc
     3721 atcaactaag gtttccagct tttcctcatt aatttcagct ttttcggcat cttctttgcc
     3781 tgtagccttg aattccatta tgattggatg atatctctta tcctttggaa ttaggattat
     3841 ataagatctt ccaagtccaa cttctaaatt agattttacg gtatatatat ttcttagtgt
     3901 ggcacataac ccgagcataa acatgtggta tgcattttct ctattatcat gataactcat
     3961 aacctgcata agcaagagct tatatctgta gacaaaaagc tcaatattgt tttccaataa
     4021 ggcctggaac atatcatata aataactgga cttcatgtta gcacttattg ttattatctt
     4081 ttcaaattcc aactgtattt catgattggg tattttaata ttatagtcta taaatcctgt
     4141 atttccctct gcaagggtta tatatccggc attcataaac aagccccaaa tattgtcctc
     4201 catatccagc atctcatagg tcatttcttt ttctattttc acctttatag cttcatagtt
     4261 taacagcttg ctgaatttat ctttaaatat gttgtctgat tttcttaagc attctattat
     4321 caaatcattt gatgcagtat ttacccagta aggctgtaat tcttttctat tgacataatt
     4381 aattgctg
//
LOCUS       DAOSDD010000311         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7059_length_4371_cov_4.805607, whole genome shotgun sequence.
ACCESSION   DAOSDD010000311 DAOSDD010000000
VERSION     DAOSDD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7059_length_4371_cov_4.805607"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK733_18145"
     CDS             complement(<1..324)
                     /locus_tag="PK733_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ12489.1"
                     /translation="MLISAIIPAYNEERTVGKIIDKLKNVDIIDEIIVVSDGSEDMTA
                     LVAKEHGATVIVLPENMGKGAAVKNGLEMSKGDIILLLDADLIGLDEMHIRNLLEPVI
                     RNECEM"
     gene            complement(377..1471)
                     /gene="ispG"
                     /locus_tag="PK733_18150"
                     /gene_synonym="gcpE"
     CDS             complement(377..1471)
                     /gene="ispG"
                     /locus_tag="PK733_18150"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOJ12490.1"
                     /translation="MELKVNKYGGADYIKRKKTKKVRAGDLFIGGDAPVTIQSMTNTD
                     TRNISETIAQIQRLEDAGCDIIRVAVPDNEAAAAIKSIKKSIKIPLVADIHFDYRLAL
                     SSMENGADKIRLNPGNIGGEERVRQVVEMAKSRHIPIRIGVNSGSVEKSIIQKYGGIT
                     PEGMVESALNHVRILENCKFDDIVISIKVSNVPMTIAAYRLMSEKTDYPLHLGVTEAG
                     TIFKGTVKSAIGIGCLLAEGIGDTIRVSLTGDPIEEVKMGHEILKALKLEENGVEIIS
                     CPTCGRCSINLADIANKVEKKLENCNKKIKIAIMGCGVNGPGEAREADIGIAGGKREA
                     LLFKRGEIIRKIPEERIVDELLEEIRKMQI"
     gene            complement(1595..2890)
                     /gene="rseP"
                     /locus_tag="PK733_18155"
     CDS             complement(1595..2890)
                     /gene="rseP"
                     /locus_tag="PK733_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOJ12491.1"
                     /translation="MNLILAFIAFNFIVIVHELGHFFVAKLTDIKVLEFSLFVGPKLF
                     SIKKGETTYSLRLLPILAYVKMEGEEEESDSERAFNKKPIWTRAAVIAAGPLANLVIA
                     VIVLCIVFSVTGFTTNRLSNVEEKSPAYNAALKEGDIILKYDGKSVYQPMDLIQFLYV
                     YKGRPAEVEYNRGGQRFTTSISPTVIPEQERYLFGFNAKQINGELSNVVGAVASDTPA
                     AKAGLQDGDVIKKLNQSPISSKKDIDHFMADNKGNPVKMTVVRNGSEIVLDIVPYVSR
                     LPEQYYLGIEFELQKGSFPGVLKHSILFAYSTARNVAYSLGWLITGRVSLNEMTGPVG
                     IVSSIGDAVEQGTTFLSKLLYLLNITAFISIAIGATNLIPFPALDGNKLLLLLIEGIR
                     KKPIPPEKEAFISMIGFVILIALAIFTIYNDTLRLIMRR"
     gene            complement(2906..4141)
                     /locus_tag="PK733_18160"
     CDS             complement(2906..4141)
                     /locus_tag="PK733_18160"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOJ12492.1"
                     /translation="MIKRISVLGSTGSIGVQTLDVVRNLNSRSTNNKSAVYGNLDNRN
                     MNDRSLENGKHNIEVIGMAAGTNIDLLESQAREFQPEVVSVKDEKLADELRTRLKGTG
                     IDVCYGMEGLKRVSTLKNADLVLNSVVGVTGLLPTLEAIRHGKNIALANKETLVAAGE
                     IVMEEAKKYKVDIIPVDSEHSAIFQCIMGNRKKDISRLILTASGGPFRGRKAEELAKV
                     KPEEALKHPNWNMGAKITIDSATMMNKGLEVIEAKWLFNINPDKIQVLVHPQSIIHSM
                     VEYIDGAVIAQLGKADMRIPIQFALTYPERRDNNFSKLDFLKYRELTFEEPDYINFPC
                     LGLAFKAISASGTMPAVMNAANEVAVKLFLDGKIEFKCIPYIINKAMDNHKVNKNPSI
                     DDIIEVDEWARAEARAYLL"
     gene            complement(4191..>4371)
                     /locus_tag="PK733_18165"
     CDS             complement(4191..>4371)
                     /locus_tag="PK733_18165"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ12493.1"
                     /translation="CGIISQIGDLVASSIKRYTKIKDYGYIIPGHGGVLDRFDSVLFT
                     APVVYFYIIFFITSH"
BASE COUNT         1208 a          999 c          668 g         1496 t
ORIGIN      
        1 catttcgcat tcatttcgta tgacaggctc caaaagattt cttatatgca tttcatctaa
       61 acctattaaa tctgcatcga gcaaaagaat aatatcacct ttactcattt caagtccatt
      121 tttaactgct gcacctttcc ccatgttttc agggagaaca attaccgtgg ccccatgttc
      181 ctttgctact aaggctgtca tatcttctga accatcactc acaacaataa tctcatcaat
      241 tatatccaca ttcttaagtt tatcaatgat tttgccgacg gttctttcct cattatatgc
      301 agggattata gcactaataa gcatatactc tctcctttgt gtggtacttg gcaccaccag
      361 aattttgtct gattggttaa atttgcattt ttctaatttc ctcaagtagt tcatctacaa
      421 ttctttcctc aggtattttc cgtattattt cacctctttt aaaaagcaat gcttctctct
      481 tcccacctgc gatacctata tctgcctctc tggcttcacc agggccgttt actccgcagc
      541 ccattatagc gattttaatt ttcttattgc agttttcaag ttttttttct actttgtttg
      601 ctatatccgc caggttaatg gaacatctgc cgcatgtggg gcatgatatg atttctacgc
      661 cattttcttc aagttttaga gcttttaaaa tttcatgacc catttttact tcctctatag
      721 gatcacctgt cagagatact cttattgtat caccaattcc ctctgctaaa aggcacccga
      781 taccaatagc agatttcact gttcctttga atattgtacc tgcttcagtt acacccaaat
      841 gcaaaggata atctgttttt tcagacatta acctatatgc tgcaattgtc atgggaacat
      901 ttgatacttt aatggaaatt acaatatcat caaatttgca gttttcaagt attctaacat
      961 ggtttaaagc actttccacc attccttcag gtgttattcc accatacttt tgaattatgc
     1021 ttttctctac agagccggaa ttaactccta tacggattgg tatatgcctt gactttgcca
     1081 tctctactac ctgtcttact ctttcctccc cgccaatatt cccgggatta agccttattt
     1141 tatcagcacc gttttccatg ctgcttaaag caagtctata atcaaaatga atatctgcta
     1201 caagaggaat ttttattgat ttttttatgg actttatagc tgcagcagcc tcattgtcag
     1261 gtactgcaac tcttattata tcacatcctg catcctcaag tctctgtatt tgtgcaatag
     1321 tttcggaaat attacgggta tcggtattag tcatggactg tattgtaaca ggtgcatccc
     1381 caccaataaa aaggtcacct gccctgactt tttttgtttt cttcctcttt atataatcag
     1441 cccctccata tttattcact ttgagctcca tccccccctc ttatatggca tcagtagaaa
     1501 aattttccaa ccttcttgcg tttcagcgag gcgagacata tgcttacgtc tgaaaaccga
     1561 aacggtatca gccgcctatt gaggtggaag acatctatcg cctcattata agcctgagag
     1621 tatcattata tattgtaaat attgcaagag caattaatat aacaaaccct atcatggata
     1681 taaatgcttc cttttcaggt ggtatgggct tctttctaat accctctatc aggagcaaaa
     1741 gcagcttgtt tccatcaagg gcaggaaaag gaattaaatt cgtagcaccg atagctatgc
     1801 tgataaaggc agttatatta agcaggtaaa gcaatttgct cagaaaagtc gttccttgct
     1861 ctactgcatc tccgattgag ctgactattc ctacaggtcc cgtcatctca ttcagagaca
     1921 ctctgcctgt tataagccat ccaaggctat aagccacatt tcttgctgtt gaatatgcaa
     1981 aaagaataga atgttttaat acaccgggga agctcccttt ctgcagttca aattctattc
     2041 ctaaatagta ctgttccggc aacctgctaa cgtaagggac tatatccagc actatctcag
     2101 aaccgttcct tactactgtc atttttacag ggttcccctt gttatcagcc ataaaatggt
     2161 caatgtcctt tttcgatgat ataggagatt gatttaactt ttttataaca tctccatcct
     2221 gcaagccggc ttttgccgct ggtgtatcag atgcaacagc accgacaaca ttagataatt
     2281 ctccattaat ctgtttagca ttaaagccaa acaagtacct ttcctgttcc ggaattactg
     2341 taggtgatat gcttgttgta aatctttgtc caccacggtt atattccact tctgcaggtc
     2401 ttcccttgta aacatataaa aactggataa ggtccattgg ttgataaaca ctcttcccat
     2461 catacttgag tattatatct ccttccttaa gagcagcatt gtaagcaggg gatttctcct
     2521 caacattgct taatctgttg gtagtgaatc ctgttacgga aaatactatg caaagaacaa
     2581 ttactgctat aaccaggttt gccagaggac cggcagctat tacagcagct cttgtccata
     2641 taggcttttt attgaaagct ctttcactgt ctgattcttc ctcctcacct tccattttta
     2701 cataggcaag tatgggaagc aaccttaaag agtatgtggt ctcgcccttc tttatgctaa
     2761 aaagttttgg tccaacaaaa agtgaaaatt ccaatacctt aatatctgtt aacttcgcca
     2821 caaaaaaatg accaagttca tggacgataa ctataaaatt aaatgcaata aaagctaaaa
     2881 ttaaattcat atattcaatt accccctata gtaaatatgc ccgagcttct gctctagccc
     2941 attcatccac ttctattata tcatcaatag acgggttttt attcacctta tggttatcca
     3001 ttgctttatt tataatataa gggatgcatt taaactctat tttcccatcc aaaaacaatt
     3061 ttacagcaac ctcattcgca gcattcataa ctgcaggcat tgtgccggat gcacttatag
     3121 ctttaaaggc cagccccagg catggaaagt ttatgtagtc gggttcttcg aaagtgagct
     3181 ctctgtattt taggaagtcg agctttgaaa agttattatc ccttctttca ggataagtca
     3241 atgcaaattg tattggtatt ctcatatcag cctttcccaa ttgagcaata accgctccgt
     3301 ctatgtattc aaccattgaa tgtattatgc tctgaggatg tactaaaacc tgtattttat
     3361 caggatttat attaaatagc cattttgctt caataacttc gaggccttta ttcatcatag
     3421 tagcagaatc tatagttatc tttgccccca tattccaatt agggtgtttc agtgcctctt
     3481 caggttttac ctttgccagt tcttcagctt ttcttcctct aaacggaccc cctgaggctg
     3541 ttaaaattaa tcttgatata tctttttttc tgtttcccat gatacactga aatatggcag
     3601 aatgctcact atcaacagga ataatatcca ctttgtattt ttttgcttct tccattacaa
     3661 tttcaccggc agctaccagt gtctctttat ttgccagggc tatattcttc ccgtgtcgta
     3721 tagcttcaag ggtcggcaaa agcccggtta ccccaactac ggaatttaaa accagatctg
     3781 catttttcag tgtcgatacc ctttttaaac cttccatacc ataacataca tctataccag
     3841 ttcctttaag tcttgtacgc aactcatcag ccaatttttc atctttaacg gaaaccactt
     3901 caggttggaa ttcacgagcc tgactttcca gtaaatcaat atttgttccc gctgccatac
     3961 ctataacttc tatattatgt ttaccatttt ccaggcttct atcattcatg ttcctgttat
     4021 ccaggttccc ataaacagcg ctcttgttat ttgtgcttct actattcaaa tttctaacca
     4081 catctagagt ctgaactcct attgatccgg tagaacctaa tactgatatt cttttgatca
     4141 tctatttcct ccattcaaaa gtggtactcc catagagcag aaaacaaaaa tcagtggctg
     4201 gtaataaaaa atataatata aaaatacaca acaggtgccg taaaaagcac actgtcgaat
     4261 ctgtcaagaa caccgccatg tcctggtatt atatatccat agtcttttat ctttgtatac
     4321 ctttttatgg acgatgcaac caggtcccct atctgtgata ttatgccaca a
//
LOCUS       DAOSDD010000312         4364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7073_length_4364_cov_4.727547, whole genome shotgun sequence.
ACCESSION   DAOSDD010000312 DAOSDD010000000
VERSION     DAOSDD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_4364_cov_4.727547"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..2813
                     /locus_tag="PK733_18170"
     CDS             552..2813
                     /locus_tag="PK733_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12494.1"
                     /translation="MKTFIPEYMLSAFAAQGIDTSKLIYAIHTDSTNKDGNTDVYIAI
                     SNESMYILYGEEKVVKTTGARRIVAVYDAIELENYPLCQLGELTCERLLSTGRLCSEK
                     DGNIKILLVFSIGFLSDIERFMKVVKNIREGAPPLNDILIDKELFCPKCGTRYPEPER
                     KLCPKCMDKISITRRLMGFFVHYRRKVAIIFITMLAGAIFSIFSPLVGTKFLFDEVLT
                     EGGSYYGAILAAVCLIFVVRAISVALNILYSYVLAKTVPWIVQDLKLKIFEAMQRLSV
                     GFYTSKRTGSLMNRVNQDATNIYWFFVDGLPYVIVNVLTFSGVITLMCVLNIKLAAVC
                     IAILPIAIGLFRILWSIFRRFHHKNWVYNSQLNSMVSDSVNGQRVIKAFAREDTEAQR
                     FSEVGGKQAAVDIRAINTGFTAFPLIYLFMFLGQVIVTAIGGNMVLKGEITLGTLLTF
                     IAYLSMLYGPLEFMSWVSNWWARCVDSAQRVFEIIDAKPDVAEPDSPVILERIRGDIE
                     LKDVKFEYDPATPVLKNLDLSVKAGEMLGIVGKTGAGKSTMANLIARLYDVTEGSITI
                     DGINVKQLSLTQLRQSIGIVSQDIYLFIGTIADNIRYARPEASIEDVIRAAKAASAHE
                     FIMMLPDAYETKVGAGGQDLSGGEKQRLSIARTILQDPDILILDEATAAMDTETEASI
                     QKALSSLQRGRTTIAIAHRLSTLRDADRLAVISDGKVVENGTHKELMQKRGEYHKLYT
                     IQLEGLKVINMES"
     gene            2956..3501
                     /locus_tag="PK733_18175"
     CDS             2956..3501
                     /locus_tag="PK733_18175"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOJ12495.1"
                     /translation="METATQITASSLTDAIDIGMLDLKNAEFYVTPGGFTGLRYAGKD
                     YNRIALRRALPVGHPMEYISVADKENKEIGMIRSLSELSEKQRSIVIAELDNRYYCPT
                     VHELKSVKNKLGYVYFELIIGRNSFKYKKNCAVKDVNKNIRMLDDDRLVIFDVDGNRY
                     IIQSLTELDKKSLKRLEPYLF"
     gene            3600..>4364
                     /locus_tag="PK733_18180"
     CDS             3600..>4364
                     /locus_tag="PK733_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ12496.1"
                     /translation="MIFRVAGERRLTVLKAHEFQYDLDLTMQPAKGTLSFDETGQIVS
                     CIGDETLLTLSANEVKEVSVIGGVGCGLLFAKMNNDSEILLCRFTMSSMKAAGEFCKV
                     VNFYAQTKQYVIPDEKEKPVCEKCGRPLVEGMSVCLFCYNKIGLLKRSLELMRPFAGK
                     IVPAELFLALSSVMYLLVPLFSRLLIDDYLRPMKGTFQQILLLAGAMFFARAIGELIF
                     ILSSRVFNEASIQYANHLRNLAYEKIQRLSMNSLAKR"
BASE COUNT         1306 a          822 c          992 g         1244 t
ORIGIN      
        1 ttcaacaagg tggtgtattt attaatgatt ctaaagttga atccattgat tataatatta
       61 cttcagaaca attgaaaaat ggtatcaaaa tacgtaaggg aaagaaaacc taccataagg
      121 ctttgttgaa gtaaagggta tgataactca atgaaagaaa cacatgtgaa agaaatacat
      181 gcactaaaat atgcactaaa aacacttgac tttttcaaat tatagtgtta atctatatct
      241 tgttggaaca tagttccaaa aataaattgt aaaggagtgc taatattgtc aatcaacttt
      301 aatattttaa gtaattataa aataaataat attatagttt tataaagtgg caataagtgc
      361 tcatttggtc gtggtatgac ccgcacttat gtgccgggga taaccacact tatcgtctgt
      421 tcaaggaatt ctgatatttt agtctatacc agcggcaggg tgtttaaccc cgccgcttta
      481 ttgtgctaat aaccaataat agggttctga aggagcaaag cttccgaagg ttcgttttag
      541 gaggaatttt aatgaaaaca ttcatacctg agtatatgct ctctgctttc gctgcgcagg
      601 gcattgacac ttccaaactt atatacgcga tccacactga ttcaaccaat aaagatggga
      661 ataccgatgt ttatattgct attagcaatg aaagcatgta tatcctttac ggagaggaaa
      721 aggtagtaaa aacaactggt gcccgcagga ttgtcgctgt ttatgacgcc atagaacttg
      781 aaaattatcc cctttgccag ctgggtgaac tgacatgtga acggttattg tcaacaggtc
      841 gcctgtgcag tgaaaaagat gggaacataa agatactgct ggtattctca ataggatttc
      901 tatccgacat tgaaaggttc atgaaagttg ttaaaaacat ccgtgaaggt gctccgcctc
      961 ttaatgatat actcattgat aaggaactct tctgcccgaa atgcggcaca aggtatccag
     1021 aaccggagcg taagctatgc ccgaaatgca tggacaaaat ctcaataacc cggcgcctga
     1081 tgggtttctt tgtacattat agacgtaagg tggctatcat cttcatcact atgctggcag
     1141 gagcgatttt ctccatattc tcgcctcttg tgggtacaaa gtttttattt gatgaagtac
     1201 ttacagaggg cggcagttat tatggcgcta tccttgcagc tgtatgcctg atatttgttg
     1261 tcagagccat cagtgtagcc ttgaatattc tctattcata tgttctggca aaaactgttc
     1321 cctggattgt tcaggattta aagcttaaaa tctttgaagc catgcagcgg ttgtctgtag
     1381 gattctatac atccaagcgc accggctccc tgatgaatcg ggtcaatcag gatgccacaa
     1441 atatttattg gttttttgtt gatggtcttc cttatgttat tgtcaatgta ttaacgtttt
     1501 cgggtgtaat aactttgatg tgtgtgctta atataaagct tgctgctgta tgtatcgcca
     1561 tacttcccat agcaatcggc ttattcagaa tcctatggag tatcttccgc cgctttcacc
     1621 ataagaattg ggtatataac tcccaactca attccatggt tagtgattca gtgaacggac
     1681 agcgtgttat caaagctttt gccagggagg atactgaggc acagcgtttt tccgaggtgg
     1741 gaggcaagca ggctgctgtg gatatacgtg ctataaatac gggctttacg gcttttcccc
     1801 ttatttactt gttcatgttt ttgggccagg tcattgttac tgctatcggc ggcaacatgg
     1861 ttttgaaggg ggaaatcact ctaggtactc tattgacatt tattgcttat ctatctatgc
     1921 tttatggacc tcttgaattc atgtcatggg tttccaactg gtgggcccgt tgcgttgatt
     1981 cggcccagcg ggtttttgaa atcatcgatg ccaaacccga tgttgctgaa cctgatagtc
     2041 ctgtcatatt ggaacgtatc aggggtgata ttgagctcaa ggacgtgaaa tttgaatatg
     2101 atcccgcaac acctgtgcta aagaacctgg atttatccgt gaaggctggg gaaatgctgg
     2161 gcatcgttgg aaagaccggt gcgggcaaat ccaccatggc taacttgatc gcacggcttt
     2221 atgatgtgac ggagggttct attactattg acggaatcaa tgtaaagcag ttatccttga
     2281 cccagctgcg gcaaagcata ggcattgtat cacaggatat ttatctcttt atcggtacaa
     2341 tagccgataa tataaggtat gcaaggccgg aagcaagcat agaggatgtt atacgggcgg
     2401 ccaaggcagc ttcagcccat gaatttatca tgatgctccc ggatgcctac gagacaaagg
     2461 tgggggcagg agggcaggat ctctcaggag gagaaaagca gcgtttatcc atagcccgta
     2521 caattttgca ggatcctgat attttaattt tagatgaagc tactgcagct atggatactg
     2581 agactgaagc aagtatccaa aaagctcttt ccagtctgca aagagggcga acaactattg
     2641 caatagctca caggctttct acgctgcgtg acgcagatcg tcttgccgtt atcagtgatg
     2701 gcaaggtggt ggaaaacgga acccacaaag aacttatgca gaaaaggggc gaatatcaca
     2761 agctctatac aatacagtta gagggcctca aggttataaa tatggaaagc taaaagaaat
     2821 aagatgagaa ataagatgaa aatagatcaa aaataagatc aaaaataaaa tcaggaataa
     2881 gaatagaaac aagagccgaa ataagaaaga aataagaagg gaattaagaa aagaaataat
     2941 taaggagtga tttctttgga aacagcaaca caaataacag catcatcttt gactgatgct
     3001 attgatatcg gtatgcttga tctcaaaaat gccgaattct atgtaacacc gggtggattc
     3061 acaggattaa gatacgccgg aaaggattat aaccgcattg cattaaggcg tgcccttcct
     3121 gtagggcatc ctatggaata catttcggta gctgataagg agaacaagga aataggaatg
     3181 atccgttccc tttcagagct cagtgagaag caacgcagca ttgtgatcgc cgaacttgat
     3241 aaccggtatt actgtccaac tgtgcacgag ttgaagtcgg taaaaaacaa gctgggttat
     3301 gtttactttg agcttataat tggtcggaac agctttaaat ataagaaaaa ctgtgctgta
     3361 aaggatgtaa ataagaatat ccgaatgctg gacgatgaca ggctggtcat atttgatgta
     3421 gacggtaacc ggtatatcat ccagtcactg accgagcttg acaaaaagag cctgaaaagg
     3481 cttgaaccat atctttttta attgaaatta ggaattgaaa ttaggaattg taactaggaa
     3541 ttgtaactag aacgttgctt acccttttcc cggcgaccag gggtaaacaa cattgaacca
     3601 tgatatttcg tgtcgctgga gaaaggcggc ttaccgtgtt gaaagcacat gaattccaat
     3661 atgatctcga cctaaccatg cagcctgcaa aaggcaccct tagcttcgac gaaacgggac
     3721 aaatcgtttc ctgcatcggc gatgaaacac tgttgacact gtccgcaaat gaggttaagg
     3781 aggtgtcggt aatcggaggg gtgggctgcg gtcttctttt tgctaagatg aacaatgaca
     3841 gtgaaatcct tctctgccgc tttacaatgt cgtccatgaa ggcagcgggt gaattctgca
     3901 aggtggttaa tttctatgct cagacaaaac aatacgtcat ccctgatgaa aaagaaaagc
     3961 ctgtatgtga aaaatgcggt cgtccccttg ttgagggaat gtccgtatgt ctattctgct
     4021 ataataagat aggacttttg aaacgctctc ttgaattaat gcgccccttt gctggtaaaa
     4081 ttgtgccggc agagcttttt ttagctctat cgtccgtaat gtaccttctc gtaccgcttt
     4141 ttagcaggct tctcattgat gattatttgc gtccgatgaa ggggacattc caacagattc
     4201 tcctgctggc aggcgccatg ttttttgcca gagctatcgg agaacttatt tttatcctta
     4261 gttccagagt ctttaatgag gcaagcatcc agtatgcaaa ccatttaagg aatctggcgt
     4321 atgaaaagat tcaaaggctg tctatgaatt cacttgccaa gagg
//
LOCUS       DAOSDD010000313         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7115_length_4343_cov_4.364272, whole genome shotgun sequence.
ACCESSION   DAOSDD010000313 DAOSDD010000000
VERSION     DAOSDD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_4343_cov_4.364272"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1372
                     /locus_tag="PK733_18185"
     CDS             197..1372
                     /locus_tag="PK733_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOJ12497.1"
                     /translation="MNVDWEQRIDWAAVRKHRLQRVVDEIKANDLDAVAFTKLDTIRY
                     AISFRSVFSLWFHGNRYILIITRDGKVKFLVASGCYERVKTSMPWITDFVAFPFVMLH
                     GVKIVQDAFKQLGLLNAKVGLDMLPFNVFSEMQRTMPNVKFQDGTPVIDKARRIKHPD
                     EIKAIRLAAEIVDIGMETVMSMNKPGVTEMEISAAASYAMNLAGSEETPYMPLVCSGD
                     HGTIGYRFPTDKRLRNGELLYSDCGAAVLNGYQGDIARTTVVGGKATNEQKKIYRCMV
                     DMLLTGQNSVRAGVSTAEIAQACQGAADKVGLGQYTYLGIVGHGIGTDIHEAPSIGDK
                     VGEGEAVEHLIENEVICLEPGILLPGIGGGHVENMILVKKNGCEPLTKTAFDDRLLE"
     gene            1389..2162
                     /locus_tag="PK733_18190"
     CDS             1389..2162
                     /locus_tag="PK733_18190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ12498.1"
                     /translation="MYPELRNKVVVVTGSAGGIGKEIAKLFSREGAIVCLLDKISTEK
                     VAKEIELFGGKCKSYIVDIINEESVTDCFESIIREIGGIDILVNNAGVLIASELLQTS
                     LESWNKMLSLNLTSVFLCSKASIPSMIQRGGGCIINAASWAAKMPSIGHGGYGAAKAG
                     VLNLTATLAGELARYGIRVVAYMPGVVRTPLSQGIVDKNPLAVANTIALGRIAEPIEI
                     AKVVVFLASDTAAYIDGTTIEISGGKLCVQNPIPLGKEV"
     gene            2184..3410
                     /locus_tag="PK733_18195"
     CDS             2184..3410
                     /locus_tag="PK733_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ12499.1"
                     /translation="MRRKLRLATFFAMLVILAMPAIAQDVIKIGTIFPRSGANAMLGE
                     QAFRGADLARRFVNEAGGVKGKQIQFVNADAPTPQAAVTEAERLISKEGAKVLIGSLT
                     SGNALSIASVTERSGAILWETSGISDDVTNKGFTSVFRTCDAGSLRGQSAVMLAYDSI
                     AALLGIPANGLRVAMIYENSSYGTSQYNGAMAEMKRRNMKFVFANGYLSSGTDQTSLV
                     LQVKQAKPDVLIHVGYINDVQLIANGLRGYKAAPKVLIGGGAGYTDPQFQVGQQTFAN
                     GVIGIDMPSNIDPNLLKNEKDRAISKQFRDRYRAEYGESPSLAAEVVFLGSYVLLNNV
                     LPAATDMSVESIKKAALSINVPETINGWAVRFDSATGQNIGATAVAYQWQEGQKQIVW
                     PSNLKSADFKYLPLSW"
     gene            3446..>4343
                     /locus_tag="PK733_18200"
     CDS             3446..>4343
                     /locus_tag="PK733_18200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ12500.1"
                     /translation="MLPVARLSTYSYFRKKGMMVSQIAQLIVSGVLAGGLYGIISVGL
                     TLIFGVLNLVNFAHGEFLMISMYISFWMSEIFNLNPYMSLPIVVAAMFVFGIIIQSIF
                     IRPVTEASHEVQIVITLGLSTIMQNSALMAFSGNLRVLRTPFSESMIEIAGIFINTQR
                     LLTFTLAILATIILYLYLTKSYFGKAIRATAQDSRAAKLAGINVPFVYMITYGIGIAL
                     VGLAGASIIPLFPVYPTIGWHFANIAFVAVVLGGLGSIPGAMVGGIIIGLIESIAGFY
                     FGSEFQQAAYFIVFIIILIIRPT"
BASE COUNT         1267 a          744 c         1015 g         1317 t
ORIGIN      
        1 gtaaatattt attaaaacag agaccgagaa tcgaacccga tacctgcgca ttacaagtgc
       61 gcctataaag ccgattactc caaatctgaa aaatttccct tgacaaccat aagaaacgtt
      121 ttataattcc ctggtaattt gtatatacaa ttatacaaaa tatgcttttt tatctataag
      181 ggggaattat atgaacatga atgttgactg ggaacagaga atagattggg cagcagtaag
      241 gaaacatcga cttcagaggg ttgttgacga gattaaggct aatgatctgg atgctgtggc
      301 ctttacaaag ctggatacaa ttaggtatgc gatatccttt cgatctgttt ttagtctttg
      361 gtttcatggc aatcgatata ttttaatcat cacgcgcgat ggaaaggtta aattccttgt
      421 ggcaagtgga tgttatgagc gtgttaaaac atcgatgccc tggattactg attttgtagc
      481 atttcctttc gttatgcttc atggtgtgaa gattgttcaa gatgccttta agcagttagg
      541 tctgcttaat gcaaaagttg ggcttgatat gcttccgttt aatgtttttt ccgaaatgca
      601 acgtaccatg ccaaatgtta agtttcagga tggtacacca gtaatagata aggctcgaag
      661 aataaagcat cctgatgaaa tcaaagctat tagattagca gctgaaatag tagacatcgg
      721 aatggagaca gttatgtcca tgaataagcc tggcgtaacc gaaatggaaa tttctgctgc
      781 tgcatcatat gccatgaacc ttgctggctc tgaagaaacg ccatacatgc ccctagtgtg
      841 ttctggagat catgggacta tcggctatcg ttttccaacc gataagcgtc taaggaatgg
      901 tgaactgtta tacagtgatt gtggggcagc tgtgctcaat ggttatcaag gagatatcgc
      961 acgtacaacc gttgttggag gaaaggcaac caacgaacag aaaaaaattt atagatgtat
     1021 ggtggatatg ttgcttacag ggcagaattc tgtccgtgcg ggtgtttcaa ccgctgaaat
     1081 tgcacaagca tgtcaaggcg ctgcggataa ggtaggcctg ggccagtata cttatcttgg
     1141 aattgtaggg cacggtattg gaactgatat tcatgaagcc ccttctattg gggataaagt
     1201 tggagaaggt gaagcggttg aacatttgat agagaatgaa gtcatatgtc ttgagccagg
     1261 aattttatta ccgggaatag gcggaggtca tgttgaaaat atgatattag ttaaaaagaa
     1321 tggatgtgag cctttgacca agacggcttt tgatgatcgg cttcttgagt gatcggcagg
     1381 tgataaaagt gtatcccgag ctgcgaaata aagtagttgt tgttaccggc agtgctggcg
     1441 gtattggaaa agagatagca aagctatttt ctcgagaagg tgctatcgta tgtcttttgg
     1501 acaagatatc gaccgagaaa gttgcgaaag aaattgaatt attcggaggt aaatgcaagt
     1561 cttatatagt cgacattatt aacgaagaat cagtcactga ttgttttgaa tccataataa
     1621 gagaaatagg cggtattgat atcttagtta ataatgccgg tgtcttaata gcttctgagc
     1681 ttttgcagac gagccttgaa tcatggaaca agatgttgtc tttaaattta acatcggttt
     1741 ttttgtgctc aaaagcctct attccatcca tgattcaaag aggtggaggt tgcataatta
     1801 acgctgcttc atgggcagca aagatgcctt caataggcca tggaggctat ggtgctgcta
     1861 aagcaggagt attaaacctt acagccacat tagccggtga attggctcgt tatgggatta
     1921 gggttgtggc ttatatgccg ggagtggttc ggacacctct atcccaagga atagtcgata
     1981 agaacccttt agcagttgct aatactattg ctttaggtag aatcgctgaa ccgatagaaa
     2041 tagcaaaagt tgtagtcttc ctcgcaagtg acactgcggc atatatcgat ggtacgacaa
     2101 tcgagattag tggtgggaaa ctttgtgtgc agaatcctat ccctcttggt aaagaggttt
     2161 agaaaattta aggaggcggc ttaatgagaa gaaaacttcg tcttgccaca ttttttgcta
     2221 tgctagttat tctagcaatg ccggcaattg cacaggatgt catcaaaata ggcacgatat
     2281 tccctcgttc cggagccaat gcaatgcttg gtgaacaggc ttttcgagga gctgatttag
     2341 ctcggagatt tgttaacgaa gcaggaggtg taaagggtaa gcaaattcag tttgtaaatg
     2401 ccgatgctcc tactccacaa gctgcagtta ctgaagcgga acgcttgatt tctaaagaag
     2461 gcgcaaaggt tctgatcgga tcacttacta gtggtaatgc actctcaatt gcttctgtta
     2521 ccgagcgttc tggagcaatt ctatgggaaa catcaggaat aagtgatgat gttacaaata
     2581 aaggttttac cagtgttttc agaacttgcg atgctggttc gcttcgtggt caatcggctg
     2641 taatgcttgc atatgatagt atcgctgcat tacttggcat tccagcaaat ggcctaagag
     2701 ttgccatgat ctatgagaac tctagttatg gaacatctca gtataatggt gccatggctg
     2761 agatgaaaag aagaaatatg aaatttgtat ttgctaatgg atatcttagt tctggcactg
     2821 accaaaccag tcttgtcttg caagttaagc aggctaagcc agatgtactt atacatgtag
     2881 gctatataaa tgatgttcaa ttaatcgcca atggattacg tggatataag gccgctccaa
     2941 aagtattaat aggaggaggg gctggatata ccgatccaca gttccaagtc ggtcaacaga
     3001 cttttgctaa tggtgtaatt ggtatagata tgccatcaaa tatcgacccc aatcttttga
     3061 aaaacgagaa agatcgagcc ataagcaagc agtttagaga taggtatagg gcggagtatg
     3121 gtgagagtcc atcacttgca gcagaagttg ttttcctagg atcttatgtt ttattaaaca
     3181 atgttcttcc tgcagccact gatatgtcag tggaatctat aaagaaagca gctctatcta
     3241 taaatgttcc agaaacaata aatggttggg ctgttcgatt cgattcagca acagggcaga
     3301 atatcggtgc gacggcagtt gcttaccaat ggcaagaagg tcaaaaacaa attgtatggc
     3361 cttctaatct taagagtgcg gattttaaat atcttcctct ttcatggtaa gtagaaggta
     3421 gctgggggct tggttattca gagccatgct cccagttgca agattaagta cctatagtta
     3481 tttccgtaaa aaggggatga tggtgagtca gattgcccaa ttaattgttt ctggcgttct
     3541 cgcgggggga ttatacggaa ttatcagtgt tggccttacc ctcatttttg gtgtgctaaa
     3601 tctagtaaat ttcgcacatg gcgaattcct gatgataagc atgtatatct ctttttggat
     3661 gtcagaaata tttaacttaa acccttacat gtccttacca atagtagttg ctgccatgtt
     3721 tgtttttggt attattattc agagcatatt tattcgacca gttacggaag cctcacatga
     3781 agtccagata gtaattactc ttggtttaag cacgataatg caaaactcag ccttaatggc
     3841 atttagtgga aatcttagag ttttaaggac cccgttttca gaaagcatga ttgaaattgc
     3901 gggaattttt ataaatactc aacgattact tacttttact cttgcaattc tggctacaat
     3961 aatactttat ctatatttga caaaaagtta ttttggaaaa gcaatacgtg ctacagccca
     4021 agattctcgt gctgcgaaat tagcgggtat taatgttccc tttgtatata tgattaccta
     4081 tggcataggt attgcattag ttggtttagc tggggcgtcc ataatcccgc tatttccggt
     4141 ttaccctaca ataggatggc attttgcaaa tattgctttc gttgcagttg tactcggagg
     4201 attgggaagt attcccggtg ccatggtagg aggaataata attggcttaa ttgaatcaat
     4261 agcaggcttt tattttggct ctgaatttca gcaagcagct tattttatag tgtttataat
     4321 aatactgatt attcggccta cgg
//
LOCUS       DAOSDD010000314         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7121_length_4341_cov_5.153523, whole genome shotgun sequence.
ACCESSION   DAOSDD010000314 DAOSDD010000000
VERSION     DAOSDD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_4341_cov_5.153523"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PK733_18205"
     CDS             complement(<1..1488)
                     /locus_tag="PK733_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ12501.1"
                     /translation="MMHLRIRNFYNQLNLTAKSISIFDKYFDKYNKKNKTRECSNYVK
                     RLQLSHLNKLFISYLMIVIICVGCMALFSYRLERENIKNWAIKSNNELLNNFKNTIDS
                     FVFDSVNKLSLIVLQNAITNSDLSFYFLNSVEGHYSDLTKVCSFLNNIRATNPLISSL
                     SIYYKSNDLLVTTDGIKYSSNDHSILPDRSYIRDLYDSDVMEYWGLKKEILATTSDEE
                     HVYIAFVRKISPPASESKSGGSIAIAVDENMLHTVIKSSAPVAFGQIFIVDEEGQIVS
                     HNEKKYLFSNINNMPYGKYILKSINKSDYFITKIDNVDSIVSYVTSDFNSWKYITVQP
                     IAELVKSRLNFLGRTILLITVVTLLFGLFASFISTRKIYSPLRNLLDLCKNVVKFRSP
                     SISKDEYGIIGSTLDILSERVKEQEKKMEQSIPIIKHHFIQNLLNSSYYNGEIIFDKM
                     EFLGISFYHDYFRVITLKLAEYPPEIDFKIYEMVKLDVMEKIGCYF"
     gene            1768..3441
                     /locus_tag="PK733_18210"
     CDS             1768..3441
                     /locus_tag="PK733_18210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12502.1"
                     /translation="MQKAVRCIALAMVIVFSAMVMTTGCVVRESGGETRSEVSKPADT
                     VKDATGKSTGEPKVEPPVKLKMFFGDAGIKFPDDIDKSNNPFLNIIEKAANVDLEMIQ
                     PAYADFQTKFNLTMASGDIPDIVHCWFHADINKYGADGAFANWEPLVKNSKVLSKYYT
                     QDMLDMMKTDDGSVYGLYVLNTRNAGATYARIDIIKEILLGDANANNDGWYNQLVTPD
                     DWYQVMKKQKEKYPDSVPFSCTGCFMMADMFFKAFGMGHDGNGVKMQVIPNEKYIWGF
                     EAPKAREAVAFHRKLYEEDLLDKTFVTNTGTEFSNRVYEKDMIVFRGDPAAVISAQTE
                     FAKRGNTKAVIGPVHNPIAPGVDPESAYWGKSPLGWHIVSINKKSPHIDACIRVFESL
                     IDPDICKQMAWGREGIEYFLRTDGSKVLDIEASNDTSYRVAYTFMRAYWFLDNLDVRA
                     AQMLPKLDATQATAFKKAWDTGIQIWEKESSQVPEITVANFVPSIPDLAPKTKEAREI
                     SKGIIFKAVMGEISMEEYDNQVKDFLSKYGYITEAYTKSYKEVLKTRGQ"
     gene            3585..>4341
                     /locus_tag="PK733_18215"
     CDS             3585..>4341
                     /locus_tag="PK733_18215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12503.1"
                     /translation="MAEVNTLDANAGTKRAKYLYSSKMRFMPGRDIPVYLLLAPAVIL
                     LFIFHYMPIYGIVMGFQKYSPFKGIGGSEWVGLLHFKEFLTDPSYWKVMKNTIIINVY
                     SLIFGFPAPIVFALLLNEVTRMKYKKIVQTVSYLPYFISWVVAAGLVMSVLSPVTGII
                     NIALNRLFRIDPIYFMVKKEYFRTILITSGIWKGVGMSSVYYLAAISSIDPTLYEAAT
                     IDGANKWQQTYHITLPGLASIITVLLILQVGSLL"
BASE COUNT         1427 a          703 c          821 g         1390 t
ORIGIN      
        1 gaaataacaa ccgatttttt ccataacatc aagtttgacc atttcataga tcttaaagtc
       61 tatctcagga ggatattctg ccaatttcaa ggttattacc ctgaagtaat catgataaaa
      121 gctaattcca aggaactcca ttttgtcaaa tattatctcc ccgttataat aactggaatt
      181 aagcaggttt tgaataaaat ggtgttttat tatagggata ctttgctcca ttttcttttc
      241 ctgctctttt actctttctg acagtatgtc cagcgttgaa cctataattc cgtactcgtc
      301 ttttgatata cttggagaac gaaattttac aacattttta cataaatcca gtaaatttct
      361 cagcggtgaa taaatcttcc tggtagatat aaaagaagca aaaagtccga ataaaagggt
      421 tacaacagta attagcaaga ttgtgcgtcc aagaaaatta agtctgcttt tcactaattc
      481 tgcgatgggt tgtaccgtaa tgtatttcca gctattgaaa tcagatgtta catacgatac
      541 gatagaatcg acattatcaa ttttcgttat aaaataatca cttttattta tcgattttaa
      601 tatgtattta ccataaggca tattattaat atttgaaaac aagtatttct tttcattgtg
      661 tgacacaatt tgaccttctt catctactat aaaaatttgt ccgaaagcaa caggggccga
      721 actcttaatt actgtatgca gcatattttc gtctacagct attgctatac ttcctccact
      781 ttttgattca gatgcgggtg gtgaaatttt acgtacaaaa gctatgtata catgttcttc
      841 atcagatgta gtagcaagta tttctttttt aagtccccag tattccataa catccgaatc
      901 atataaatcc cttatgtagc ttctgtcagg taaaatcgaa tgatcatttg aactatactt
      961 tataccatct gtagtaacca ggagatcatt gcttttataa tatattgata atgaacttat
     1021 taatggattt gttgctctaa tattatttag aaatgaacat actttggtaa gatcggaata
     1081 atggccttct acagagttta gaaaataaaa tgataaatcg ctattagtta ttgcattctg
     1141 taacactatc aacgacaatt tattaacgct gtcaaaaaca aaactgtcta tggtattttt
     1201 aaaattattc agtaattcgt tgttggattt gatagcccag ttttttatat tttctctctc
     1261 caaacgatat gaaaataaag ccatacaccc aacgcagatt attacaatca tcaggtatga
     1321 aataaaaagc ttgtttaaat gacttaattg taagcgcttg acataatttg aacattcccg
     1381 ggttttgttt tttttattat acttatcgaa atacttgtcg aaaatagata ttgattttgc
     1441 cgttaagttc agctggttgt aaaaattccg aattctgagg tgcatcatat aacctccctt
     1501 ctatcttaca acccctatta taacttattt taaatctttt ttaaacagca tagcaaagat
     1561 ttcacaaatt attcaacata ggtgcaagaa atttattatt gaaaaatcaa attatttaac
     1621 atagttaaaa gtattttatg ttgtgcttca ttagaatgag taatagaatg aaatcatgga
     1681 aaagtcatga aacaacaaac cacattatat gtctgatacc ctgataatgt acggggttgg
     1741 aaatataaaa tcaaggaggt agtttttatg caaaaagctg ttagatgtat tgcattggca
     1801 atggttattg tattttcagc gatggtaatg actactggtt gtgtagtgcg agaaagtgga
     1861 ggtgaaacca ggtctgaagt atcaaagcct gctgataccg taaaagatgc tactggtaaa
     1921 tctactggtg aacctaaggt ggaacctccg gtaaaactta aaatgttttt tggagatgca
     1981 ggtatcaaat tcccggatga tattgacaaa agcaacaacc cttttcttaa catcattgag
     2041 aaagcagcaa atgttgacct ggaaatgatt caacctgcat atgccgattt tcagacaaaa
     2101 ttcaatctta ccatggcttc cggagatata ccggatattg tgcactgctg gttccatgca
     2161 gatataaata agtatggtgc agatggcgca tttgccaact gggaaccgct ggtaaagaat
     2221 tctaaagttt tgtcaaaata ctatacacaa gatatgcttg atatgatgaa aactgacgat
     2281 ggttcagtat atggactgta tgttttaaac actagaaatg caggtgctac ttatgctcgg
     2341 atagacatta taaaggaaat cctgctagga gatgcaaatg caaacaatga tggctggtat
     2401 aatcaactgg ttactcccga tgattggtac caggtaatga aaaagcagaa agaaaaatat
     2461 ccggattctg tgccattctc atgtacaggt tgctttatga tggcagatat gttctttaag
     2521 gcttttggta tgggacatga cggaaacggt gtaaaaatgc aggtaatacc caatgaaaag
     2581 tatatatggg gttttgaagc tcccaaagca agagaagctg tagcattcca caggaaattg
     2641 tatgaagagg atttattgga taagactttt gttacaaata caggaactga gttttcaaat
     2701 agagtatatg aaaaagatat gattgttttt cgtggtgacc cggctgcggt aatcagtgca
     2761 caaacagaat ttgcaaaaag aggcaataca aaggctgtta taggtcctgt gcataatcca
     2821 atagctccgg gagttgatcc tgagtctgct tattggggta aatctccatt aggctggcat
     2881 atagtttcta taaacaaaaa atctcctcac atagatgcct gcataagagt gtttgaatca
     2941 ttaattgatc ctgatatatg caaacaaatg gcctggggaa gagaaggtat tgagtatttt
     3001 ctgagaacag atggctcaaa agttcttgat attgaagcaa gtaatgatac atcatacaga
     3061 gttgcctaca catttatgag agcctactgg ttcctggata atcttgatgt cagagccgca
     3121 caaatgcttc ctaaactgga tgcaactcag gcaacagcat ttaaaaaagc atgggataca
     3181 ggtattcaaa tatgggagaa agagtcctca caggttccgg aaataactgt tgcaaatttt
     3241 gtaccttcaa ttccggatct tgctccaaaa acgaaggaag caagagaaat atccaaggga
     3301 attattttta aggctgttat gggcgaaatt tcgatggagg agtatgataa ccaggttaaa
     3361 gattttctga gtaagtatgg ttatataact gaagcttata caaaatcata taaagaggta
     3421 ctcaaaacaa gaggtcagta aaaaagtgca aagattacag agcacaatta gttcaggtgt
     3481 agttcaaaaa gtgcagcgtg tgcagggcat agcttttgga gaagctatgc cctgcacctg
     3541 gaatatgtta ttctggtttt tacatttcag tggaggtggt actattggca gaagtaaata
     3601 ctttagatgc aaatgcgggc actaaaagag cgaaatatct ttatagcagt aaaatgagat
     3661 ttatgccagg cagagatata cctgtatatc tgcttcttgc cccagctgtt atattgcttt
     3721 tcatattcca ttacatgccc atatatggta ttgtaatggg ttttcagaag tacagccctt
     3781 ttaaagggat aggcggaagt gagtgggtag gattattaca ctttaaggag ttcctgacag
     3841 acccgtccta ctggaaggtt atgaaaaaca ccataataat aaacgtttat tctttgatat
     3901 ttggcttccc tgcacctatt gtatttgcgt tattattaaa tgaagttaca agaatgaagt
     3961 ataaaaagat agtacaaacc gtaagttatc tcccctattt tatatcatgg gttgttgctg
     4021 cagggcttgt tatgtctgta ctttcacctg ttacaggtat tattaatatt gctttaaaca
     4081 gattgttcag aatagacccc atttatttca tggtaaaaaa agaatatttc agaacaatac
     4141 ttattacatc cggcatatgg aaaggtgtag gaatgtcatc tgtatattat cttgcagcaa
     4201 taagttctat agatccaacg ctatatgaag ctgctaccat agatggcgca aataaatggc
     4261 agcagacata tcacattacc ttgcctggtc tggcttcaat tataactgtt ttactgatac
     4321 ttcaggtggg cagccttttg a
//
LOCUS       DAOSDD010000315         4324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7163_length_4324_cov_3.165847, whole genome shotgun sequence.
ACCESSION   DAOSDD010000315 DAOSDD010000000
VERSION     DAOSDD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_4324_cov_3.165847"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK733_18220"
     CDS             <1..708
                     /locus_tag="PK733_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOJ12504.1"
                     /translation="IFIAGAVKGPFQSVISTLALLGVIIFGIIMTLLTSRLLSKTVLK
                     GLSSSFTLELPPYRKPQIGRVIIRSIFDRTLFVLGRAAAVAAPAGLVIWVMANAHIGE
                     LSLLNHCANFLQPFAYMLGLDGYILMAFILGFPANEIVIPIIIMSYMATGSILEIDSL
                     NELRELLVSHNWTWLTAVCVMLFSLMHFPCGTTLWTIKKETQSIKWTVISFVIPTTIG
                     IVVCFTAASIVRLLGLE"
     gene            complement(939..3350)
                     /locus_tag="PK733_18225"
     CDS             complement(939..3350)
                     /locus_tag="PK733_18225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ12505.1"
                     /translation="MKRSTAKKVVRKLFITMLTCLIIGLLIYYFVSQMLMKNVETSLT
                     VPRHQVLENIRHVLLFIAFIVFIFMFILIAMYAYGIYLRYKFRKQKQISDLAVKTANI
                     LIIGLDSNWRITSFNKFAEVKTGYRKDEVVEIKTFFDLVSQDSLDKLHEMIKHVKDFN
                     PLNNCELTLIDTSGNLFFAQWNVNISSDLFGNIIGIQLIGIDITDRVLQEQELIKYNN
                     ELSNLYNEIAASEEELKQQNQELIYNQQCLEKSEERYRLSSEGSNDVLWDWQIESPMD
                     IYFSDRWYDILGYKKEDFNANIDAIKTLIEPKDYRNTERSFYQHLKGEKELFETELRL
                     KDNSGEYRWFYVRGKALLGSTGMATRIAGSITDITEKKRNEEYISNLAYYDILTGLSN
                     RIHLEEQITHAINKTNNKIAVLFIDIDNFKYINDFFGHSFGDSLLIQVGRRLTTSNRY
                     KTLASRLGGDEFIVMAQIDSREDAQNCAEEILESIGMSFEIGDVIFNIYASIGISVYP
                     NDGINFEELLKTADIAMYKAKQQGKNRYVFFDKSMSGELLEKMIMENDLKNAIDNNEL
                     IAYYQPLYNMKTGEIAGFEALARWNSPKYGFIQPKDFIPLAEEMGFIVSIDRHILKMA
                     CTFLRELHDKGFSNLIISVNISVIQLFQDDFDDMILEIVQNSRLNPKYLIIEITESVM
                     MENFEHNIGKIENIRANGVSIALDDFGKGYSSLTYLRDLPINFMKLDKAFIDDIDDIK
                     SDKQITESIIRLAHNLDLGVIAEGVETKEQLEYLLNYKCDMAQGFLMGKPMSESGIRQ
                     LLEKK"
     gene            complement(3570..4172)
                     /locus_tag="PK733_18230"
     CDS             complement(3570..4172)
                     /locus_tag="PK733_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOJ12506.1"
                     /translation="MKGRYNIIEKKIPKREEAFELLKEYNKSENLIRHALMVEAVMLH
                     FARLFNEDDVEKWGVIGLVHDLDYELYPDEHCKKVRSILEEKGWPEDCIRSIESHGWK
                     ICSDVEPVLKMEQVLYTIDELTGLVAATALMRPSKSILDLEVKSVKKKWKQKNFAAGV
                     NREIIEEGAKYLGMELNEIISETISGMQKVAESIGLKGEN"
BASE COUNT         1289 a          868 c          615 g         1552 t
ORIGIN      
        1 atattcattg ccggtgctgt taaaggtcca tttcagtcag tgatatcaac actggcacta
       61 ttaggtgtta ttatttttgg aataataatg acactgttaa cctccagact cctgtcaaaa
      121 actgttttaa aaggcttatc ctcatcattt accctggagc ttccgccata ccgcaagccg
      181 cagattggca gagttattat ccgttccata tttgacagaa ctctttttgt actgggccgc
      241 gcagctgctg ttgctgcacc tgcaggcctt gtgatatggg tcatggctaa tgcacatata
      301 ggggaattaa gtcttttaaa tcattgtgct aactttttgc aaccgttcgc ttatatgctg
      361 ggtttggatg ggtatattct tatggcattc attttgggat ttcctgccaa tgaaatagta
      421 ataccaataa ttattatgag ttatatggcc acaggctcaa tacttgagat tgatagcctt
      481 aatgaattga gggaacttct ggtatcacac aactggacat ggctaacggc tgtttgtgtc
      541 atgctgtttt cacttatgca tttcccctgt ggtaccacat tgtggacaat aaaaaaggaa
      601 acacagagta taaagtggac tgttatatct tttgtaatac caaccacaat aggaatagtt
      661 gtatgtttca ctgcagcaag cattgtacgg ctattaggac tagagtagtg aatcatttcc
      721 agctgcaata tttgtagcaa taaagaatca tttgaaagca caatgaatta cggctttcga
      781 aaatactgct caacctaaac tggaaatggc ttcttactta gaccaaacta gcaaaagcaa
      841 gcgagtggga atacctcctt ctcttatacc attatgccat ttctcacctc tcacctctca
      901 catcttacct ctagcatctt acctctagca tgtattggtc actttttttc aagcaactgc
      961 ctgattcccg attcagacat aggttttccc attagaaatc cttgtgccat atcgcactta
     1021 taattcaaca gatattcaag ctgttctttg gtttcaactc cttcagcaat aacgcctaaa
     1081 tctaaattat gggcaagcct tataatagac tcggtaattt gcttatcaga ttttatatca
     1141 tcgatgtcat caataaaagc cttatccagt ttcatgaaat taatgggtaa atcccttaaa
     1201 tatgtaagtg atgaataccc ttttccaaaa tcatccaaag ctatgcttac tccgttcgcc
     1261 cgtatatttt caattttccc aatattgtgt tcaaagtttt ccatcattac agattcagtt
     1321 atttctatta taagatactt gggattaagt cttgaattct gaacaatttc taaaatcata
     1381 tcatcaaagt catcctgaaa aagttgtata acagatatat tgactgaaat tattaagttg
     1441 ctgaaacctt tatcatgtaa ttcccgtaaa aatgtacaag ccatttttaa aatatgcctg
     1501 tctattgata ctataaatcc catttcttca gcaagaggta taaaatcttt gggttggata
     1561 aaaccatatt tgggactatt ccagcgggct aatgcctcga agcctgcaat ttccccagtt
     1621 ttcatattat ataatggctg ataatatgct atcaattcat tattatcaat agcatttttt
     1681 aaatcatttt ccatgatcat tttttccaat aattcgccac tcatgctttt gtcgaaaaac
     1741 acatatctat tcttgccctg ctgtttagcc ttatacatgg caatatcagc agttttcaac
     1801 agctcttcaa aattaatacc atcattagga tacactgata ttcctatgct ggcataaatg
     1861 ttaaatataa catcgcctat ttcaaagctc atacctattg attccaaaat ctcttctgca
     1921 caattttgtg catcttccct gctgtcaatt tgtgccataa caataaattc atctccaccc
     1981 agtctagatg caagagtttt gtaccggttt gaagttgtca atcttctacc cacttgaata
     2041 agaaggctgt ctccgaagga atgtccaaaa aagtcgttta tatatttaaa gttgtctata
     2101 tcaataaaca aaacagctat tttgttattt gttttattaa tagcatgtgt tatttgttct
     2161 tcaagatgaa tacggtttga aagccctgta agtatgtcgt aataagccag attactgata
     2221 tattcctcat ttcttttctt ttctgttata tcagtaattg agcctgcaat tcttgttgcc
     2281 attcctgtag aacccagcaa agcttttcct cttacataaa accatctata ttctccagaa
     2341 ttatctttta atcttaattc tgtttcgaat agttcttttt ctccttttaa atgctggtaa
     2401 aaactccttt ctgtattcct ataatccttt ggttcaatta gagtttttat agcatctata
     2461 ttagcattga aatcctcttt tttatatcca agaatatcgt accatctgtc agaaaaataa
     2521 atatccatag gcgactctat ttgccaatcc cataggacat catttgatcc ttctgatgaa
     2581 agacgatatc tttcttcgct tttttccaga cactgttgat tataaatcag ttcctggttt
     2641 tgttgcttta attcctcttc tgatgctgca atttcattat atagattaga aagctcattg
     2701 ttatatttta taagctcctg ttcctgcaac actctatcag taatatcaat gccaatgagc
     2761 tgaatgccaa taatatttcc aaatagatca ctggatatgt tcacattcca ttgagcaaaa
     2821 aataaattac cactggtgtc aattaaagtc agttcacaat tatttaaagg attaaaatcc
     2881 ttaacatgtt tgatcatttc atgcagctta tccaagctat cttgtgaaac taaatcaaag
     2941 aaagttttta tctcaactac ttcatctttc ctataaccgg tttttacttc tgcgaacttg
     3001 ttaaaactgg taattctcca attactatcc aaaccaataa ttaatatatt tgcagttttg
     3061 acagctaaat ccgatatttg cttctgcttt ctgaatttat aacgtaggta tatgccatat
     3121 gcatacattg caataagtat aaacataaat ataaaaacaa taaaagctat aaaaagcaat
     3181 acatgcctaa tattttccag cacctggtgt ctgggaacag taagagatgt ttctacattt
     3241 ttcataagca tttgagagac aaaatagtaa attaataatc caataataag gcaggtaagc
     3301 atagttataa acaattttct tacaactttt tttgcagtag atcttttcaa aataattcct
     3361 ccattcaata gtaacccaat atatatttcg gcataaagta attatttgtt taatacgaaa
     3421 gtggagctta aactgttgtt ttttggcttg tataattaag taagaagcca tttccagttt
     3481 gggttgggta gcattttcga aagctttaat tcattgcgtt tcattttttt ttattgttgc
     3541 aaatattgca actggaaatg gttcgctact tagttttccc ctttaagtcc aatactttct
     3601 gctacttttt gcatacccga tatagtttcg ctaattattt catttagctc catcccaaga
     3661 tactttgcac cctcttcaat tatttctctg ttaactccgg ctgcaaagtt cttttgcttc
     3721 catttctttt ttactgattt tacttccaga tccaatatac ttttacttgg gcgcataagt
     3781 gccgtagctg caaccaatcc tgtcagttca tcaattgtat acaatacttg ttccatcttc
     3841 aaaaccggct caacatcaga gcaaatcttc cagccatggc tttctataga gcggatacag
     3901 tcttcaggcc agcctttttc ttccaagata ctcctgacct ttttgcaatg ttcatcagga
     3961 tacagttcat aatcaagatc atgtaccaga cctataactc cccatttttc cacatcatct
     4021 tcattaaaca acctggcaaa atgcaacatt acagcttcaa ccattaatgc atgccttata
     4081 agattttcgc ttttattata ttcttttagt agttcaaaag cctcttccct tttcggaatc
     4141 tttttctcaa taatattata tcttcctttc ataatatatt atttatatca taaatctatc
     4201 ttaccaattt aaaatttgct tgccaatata aattgattgc tttccatgcc gagtccaaat
     4261 cctttttgat attgtcggaa tcagcatacc aaacacttat tccagctaat aaaggttctc
     4321 gctt
//
LOCUS       DAOSDD010000316         4315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7181_length_4315_cov_3.292254, whole genome shotgun sequence.
ACCESSION   DAOSDD010000316 DAOSDD010000000
VERSION     DAOSDD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_4315_cov_3.292254"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PK733_18235"
     CDS             <1..1268
                     /locus_tag="PK733_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ12507.1"
                     /translation="RGDTNKFKGEYRAIISGINNTLNAIIEPIKESSGVLQELAKGNL
                     HVNVKGDYKGDHAQIKTALNDSINTILSYISEISGVLTEMAKGDLDVEITADYKGDFV
                     EIKNSLNYIVQALNTILGDINSASEQVASGSRQVSDGSQALSQGSTEQASSIEELSAS
                     ITEIAEQTKNNAINANHANELALNAKENAILGNEQMKEMLNSMVEINESSANISKIIK
                     VIDEIAFQTNILALNAAVEAARAGQHGKGFAVVAEEVRNLAARSANAAKETTTLIEGS
                     IRKVEAGTKTANETAVELNKIVSDSEKVAKLVGEIASASNEQASAIAQINRGIEQVSQ
                     VVQTNSATAEESAAISEELSSQSELLKDMVSQFKLKNGIDDQKRITASSFEDRKKNSS
                     TAIKINRMQAAAVGSNPRIALSDKEFGKY"
     gene            1379..2206
                     /locus_tag="PK733_18240"
     CDS             1379..2206
                     /locus_tag="PK733_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOJ12508.1"
                     /translation="MNINMNIKDKEFRILTEYIYKNYGINLFQKKNLVEGRLSNIIIE
                     KGFNNFTDYIDFVLSDKTGMEATVLVNKLTTNYTFFMREASHFEYFKDKVLPYLVSSL
                     KERDMRVWSAGCSSGEEPYTLAMLLQEFFGTQKPLWDTKILATDISVKVLEEGERGIY
                     SANALKHIPPLWKMQYFNKIGNENYKISSKIQNEVIFRVFNLMDEVYPFRRKFHTIFC
                     RNVMIYFDYKTKINLVRKLYEHTEPGGYLFIGHSEFIPRETTDYKYIMPSIYRKGMI"
     gene            2208..3245
                     /locus_tag="PK733_18245"
     CDS             2208..3245
                     /locus_tag="PK733_18245"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOJ12509.1"
                     /translation="MVNNRKIKVLVVDDSLLFRETIAQGIAQDFGIEVVGTASDPFIA
                     RDKILELKPDVISLDVEMPKMNGIEFLKKLMPQYPLPVVVVSSISSNVFDALNAGAVD
                     FVTKPDINKPGGLSFFIKELIVKIKIASTARMGSYKKEHVPVKPISHTKALKDEIIAI
                     GASTGGTEAIYEVIKLFPPDMPGIVIVQHMPPVFTRMYAERLNNSCMLEVKEAQDGDL
                     VKTGRVLVAPGDFQMKVVKERGNYYVKCYKGEKVNGHCPSVDVLFDSIATAAGSQAVG
                     VILTGMGCDGAKGLLEMKRHGAYTIGQDEKSCVVYGMPRVAFNIGAVQNQAPLDRIPQ
                     IICKYIEKRET"
BASE COUNT         1529 a          647 c          884 g         1255 t
ORIGIN      
        1 taagaggtga tactaataaa tttaaaggtg aatatagagc tattatatcc ggtataaaca
       61 atactctcaa tgcaattatt gaacctatta aggagtcatc cggtgtactt caggaactag
      121 ctaagggaaa cctgcatgta aatgtaaaag gtgattataa gggtgatcat gcacagataa
      181 aaaccgcact gaatgattca attaacacta tactcagcta tataagtgaa atatcaggtg
      241 tactgactga aatggccaaa ggagatctgg atgtcgaaat tacagcagac tataaagggg
      301 atttcgtaga aataaaaaat tcgttaaatt acattgtaca agcccttaac acgatcttgg
      361 gcgatataaa tagtgcctcg gaacaggtag cttctggttc caggcaagta tctgatggca
      421 gccaggcgct ttcccagggg tcaacagagc aagccagttc cattgaagag ctttctgctt
      481 ccataactga gattgctgag caaacaaaaa ataatgcaat aaatgctaat catgccaatg
      541 aattggcatt gaatgcaaaa gaaaatgcaa tacttggaaa tgaacaaatg aaggaaatgc
      601 ttaattccat ggttgaaatt aatgaatctt ccgctaatat ttctaaaatt ataaaagtta
      661 ttgatgagat tgctttccag accaacatac tggcactgaa tgcggctgtt gaagcagcac
      721 gagcaggaca gcatggaaaa ggcttcgccg ttgtagcaga ggaggtgaga aacctggcgg
      781 caagaagtgc gaatgctgct aaagagacta cgacccttat tgagggatct attagaaaag
      841 tagaagccgg aaccaaaact gccaatgaaa cggcagtaga attaaataag attgttagtg
      901 attcagaaaa agttgcaaaa cttgtaggag aaattgcttc agcttctaat gaacaggctt
      961 ccgccattgc tcagattaac agaggcattg agcaggtatc ccaagtggtt cagacaaatt
     1021 ctgcaacagc agaagagagc gctgctatca gtgaagagct ttcaagtcag tctgaactat
     1081 taaaggatat ggtaagccag tttaagctaa aaaatggaat agacgatcaa aaaagaataa
     1141 cagcatcttc tttcgaagat agaaaaaaga attcttcaac agcaatcaaa ataaacagga
     1201 tgcaggcagc tgctgttgga agcaatccca gaattgcatt aagtgataaa gaatttggca
     1261 agtattaatt ggatatcagg aacatgaata acaggaatag tgggagccgg actgtcggag
     1321 tatcttgtat ctgacagtcc gaaacctgtt taaatggtaa tttatcatga gtataaacat
     1381 gaatataaac atgaatataa aagacaaaga gtttagaata ttaacagaat atatctataa
     1441 aaattatgga attaatcttt ttcagaaaaa gaatctggtc gaaggaagat taagcaatat
     1501 aattattgaa aaagggttta ataattttac agactatatt gattttgtgc tgtctgataa
     1561 aactggtatg gaagcaactg tactagtaaa caagttaaca acaaattaca ctttttttat
     1621 gcgtgaggca agccactttg aatatttcaa agataaggta ttgccttatc tggtttcaag
     1681 tctgaaagaa agagatatga gggtttggag tgcgggttgt tcatccgggg aagaacctta
     1741 tacattggct atgcttttgc aagaattttt tggaactcaa aaaccacttt gggatactaa
     1801 aatactggca acagatattt cagttaaggt gctggaagaa ggtgaaagag gtatatattc
     1861 tgcaaatgca ctaaaacata tacctccttt atggaaaatg caatacttta ataaaattgg
     1921 taatgaaaac tacaaaattt caagtaaaat acaaaatgaa gtaatattta gagtatttaa
     1981 cctgatggat gaagtctatc ctttcaggag gaaatttcat accattttct gcaggaatgt
     2041 aatgatctat tttgattata aaacaaaaat taatttagtt cggaagttgt atgaacatac
     2101 tgagccaggg ggatacttat ttataggaca ttcggagttc atccccagag aaactacaga
     2161 ctataaatat atcatgcctt cgatttacag aaaggggatg atctaaaatg gtgaataaca
     2221 gaaaaataaa ggtattagta gtagatgact ccttattatt tcgtgaaaca atagcgcagg
     2281 gaattgccca ggattttgga attgaagttg tgggtaccgc atctgatcca tttatagcca
     2341 gagataaaat attggagctt aaaccggatg taatctcact cgatgttgaa atgccaaaga
     2401 tgaatggtat tgagtttctg aagaagttga tgcctcaata ccctctaccg gttgtagttg
     2461 tcagttccat aagcagcaat gtatttgatg cacttaatgc cggagcagta gactttgtta
     2521 ctaaacctga tataaataaa cctgggggat taagcttctt tattaaggaa ttgattgtta
     2581 aaattaagat agcttcaaca gccaggatgg gaagctacaa aaaagaacat gtacctgtaa
     2641 agcctattag tcatactaaa gcattaaaag atgaaatcat tgcaattggg gcttcaaccg
     2701 ggggaacaga ggctatctat gaagtgataa agttatttcc gccagatatg ccagggattg
     2761 ttatagtcca gcatatgcca cctgtattta caagaatgta tgcggaaaga ttgaacaatt
     2821 cttgcatgct agaggtaaaa gaagcccagg atggagattt ggttaagaca ggaagggttc
     2881 ttgttgctcc gggagatttt caaatgaagg tagtaaagga gcggggaaat tactatgtaa
     2941 aatgctataa aggagagaaa gtcaatggac actgtccatc tgtggatgtt ttgtttgatt
     3001 cgattgccac tgctgcaggc agtcaggccg taggtgtgat tctaacaggg atgggttgcg
     3061 atggagccaa aggattgctg gaaatgaaaa ggcatggtgc atataccatt gggcaggatg
     3121 aaaaatcgtg tgttgtatat ggaatgccaa gagtggcttt caatatagga gcggttcaaa
     3181 atcaggcacc tttagatagg attccgcaga taatttgcaa atatattgaa aaaagagaaa
     3241 cataggtgta ttattaattt attgtataat tatgacacaa aaacttattc agcgtcgacg
     3301 tgttgaaaat atgtgtattg ttaacacaat gaaattagca atactttggt atgtgagata
     3361 cactttagct gaaagcgttt atatcaattg aagggaaaat gtttgaatgg cattaattta
     3421 ctgcaacttt tactcagaag tactgcaaat tgcttcatca atgtatgtaa ttctacccct
     3481 aagcgtgcac acccagtgca caacagtgca tcattacccc tgaatagtta ccaaaaaaac
     3541 gtcaaaaaat ataatatttt gtctaaatat tgtattatgt aaatagtatg gtataataca
     3601 atgggatgga cttttagtga aggtgaggta tgctagtaat aatggtaaag aaaatgccat
     3661 tttatctatt ttttatattc attcttatat tagctattac aagtattctc ttttctgtac
     3721 agttaattgg cacctcatat tcattacaaa catatgacga ttgcagtatt gaaagcttgg
     3781 tcactatagc tgataattac acccctgaca cattaaaaca gcgtaccgta agaattaatc
     3841 tatttgcact tccttattct atgcaatgca agataatatc taatcccaaa atatcatatt
     3901 ttgtactaca tgcaaatctt tgttatcaaa tccaaagatt atatattcat attgccagat
     3961 taaaagctgg gtaatttata tgtaaggtat tttatggtac cgatagtagt tgctataatg
     4021 caatcatgat ttaatcatat taatcggaat actactgtaa aaatttgtag agtatctacc
     4081 tttgcattga aatcagtcaa ataactgggg aaagtcatta tacagtataa atatgcttag
     4141 gattccctgg tgaacaagaa aatttacttc gatattcaag ctataagcta atagaatttt
     4201 cttaggtaac cagattgttc taaacaaggc tgcaaactgg ttgctttgct ttgcttacac
     4261 agaaattagt aaaagctttg cctgaattat atggtatata ttgcatgtac tgatt
//
LOCUS       DAOSDD010000317         4275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7277_length_4275_cov_6.885071, whole genome shotgun sequence.
ACCESSION   DAOSDD010000317 DAOSDD010000000
VERSION     DAOSDD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7277_length_4275_cov_6.885071"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1005)
                     /locus_tag="PK733_18250"
     CDS             complement(133..1005)
                     /locus_tag="PK733_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12510.1"
                     /translation="MNYKHIVLHGYLECKPEKTAFGKYFERQAKIAERDEFVEKQDFY
                     GECLSVIKSYQDEVYYQYRKYLTEHDSALAGYRTVLKNGRTKDDKGVAIQTHIDNILQ
                     ERKGKEQQGYENDTYYFWCGTDGNGKIVNDMFSLEHKLYYPILERMKESINILIISIS
                     EQSTQDNDSTPGDSEINLSNWGRNDKTRYEYDPITISRIFEHCQKTDVFDDQISLSDF
                     TMAIDNTDFKEIVEKCAKRKKKSKCMSVINKLSKVISSEWYRKTAHSINTEPTRISGI
                     SVPCGWKKELDDIK"
     gene            1227..2555
                     /locus_tag="PK733_18255"
     CDS             1227..2555
                     /locus_tag="PK733_18255"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOJ12511.1"
                     /translation="MQYKNSKKISFTAGSVKKEFSSEQLTSYSGLSVTSDFINHCGIY
                     RRLEHLFPTTRHNASRFSTAQILSSILLASLCGVHRLKRIENFTFDALVSRLLKLPKN
                     IDQDTIRRHLTGLGERGARSLHELLLGFTGMQVSHCGLSRLTLDCDSSTFTVYGNQQG
                     AEVGYNSHKKGSKSYHPILCFVTEMKLLVNSWLRPGSAYTSNGVCEFVKETLAALPQK
                     VEKVFFRADSGFFNGGLFNLLEDGKHEYLVKVKLKNLKDLLAGQTWQPIGPRTATCQF
                     THQCSGWRNPRMFYAVRVVKQMVEVDYFGEKQFVPEYEYFCYCSNLKGLDALQLHTLY
                     GSRSESENWIEQTKNSLCAGKTITHDFWVNDILWQLSSFAYSLSVLMRYRGDFWVWRQ
                     EHSTFREWFIRVPGKVVKSGRQVTVKMPKEYYRKAGWRDFEQRITTTMTG"
     gene            complement(2998..3525)
                     /locus_tag="PK733_18260"
     CDS             complement(2998..3525)
                     /locus_tag="PK733_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12512.1"
                     /translation="MDKATIHMMNINMNKEENKQYIYHQPEVIETDSFKKWEYWLYPE
                     TAPFNTKTRCGTVYRTPMTDRDRADIYQFKEQIQIIIASGNNDLSDRTNRIIELAAKH
                     QNDFKNFDSFSKYYFLFECSPENPILMEELSKIIMKLTGLVKDEGLFPSDYFWATESK
                     DGLGKILKINIQGGR"
     gene            complement(3691..>4275)
                     /locus_tag="PK733_18265"
     CDS             complement(3691..>4275)
                     /locus_tag="PK733_18265"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase domain-containing protein"
                     /protein_id="HOJ12513.1"
                     /translation="DTHNQHRQLGNLPAADVIIHSGDITFAGTGDEVLDFIEWFGSLS
                     YQYKIFIAGNHDYALEGKAPERIQRFLPENCFYLYNSGITIYEIHFWGIPFFFSPDEP
                     KSDTKAFDLVPDDTDVLITHRPPFGIQDQSSNTRFGCSNLLQAVTRICPAYHLFGHIH
                     DAYGIEKHKEITFSNAAVVDADYRLVNDPILFEL"
BASE COUNT         1246 a          829 c          835 g         1365 t
ORIGIN      
        1 gagggtgggt gtagggtgta ttagattatt ttcaaaaaaa taaccccttg atatatatga
       61 atatcaaggg gttaacataa tttaagcaat gaattaacag ggtgtaacca gggtagccct
      121 ataaatatta ttctatttta tatcatctag ttcctttttc cagccacaag gaacagaaat
      181 accgctaata cgtgtgggtt cagtattgat gctgtgagct gtttttctat accattcaga
      241 actgattact ttcgagagtt tatttatcac agacatacac tttgactttt ttttcctctt
      301 agcgcatttt tcaactatct ccttgaaatc agtattatct atagccatag taaaatcaga
      361 taatgatatt tgatcgtcaa aaacatcagt cttctgacaa tgttcaaaga tcctggagat
      421 ggtaattgga tcatattcgt atcttgtctt atcgtttctg ccccaattac ttaagttgat
      481 ttcactatcc ccaggagtag aatcattatc ctgcgtagac tgttctgata tcgatattat
      541 gagtatatta attgactcct tcattctttc aagaatggga tagtatagct tgtgttcaag
      601 ggaaaacatg tcattaacaa ttttgccatt gccatcggtg ccgcaccaga agtaatatgt
      661 gtcattttcg tatccttgct gttcttttcc tttcctttct tgtaaaatat tatcaatatg
      721 agtttgtata gccacaccct tatcatcttt cgttcttcca ttcttcaata cagttctata
      781 gcctgctaat gcgctatcat gttcagttaa atactttcta tattgataat atacttcatc
      841 ctgatagctt ttaattaccg ataggcattc cccataaaag tcttgtttct ccacaaactc
      901 atccctttct gctatcttgg cttgtctctc aaaatatttg ccaaaggcag tcttttccgg
      961 cttacactcc aaatatccgt gtaaaacaat gtgcttataa ttcataaaca ataatctaaa
     1021 ataatatcta ttgctttgct atgcaaatgt atgaaatcat ttgaaacagt gactgagaaa
     1081 ttggagatag ttatttacta gatgcttatt tatgttgtta cttattgaaa taatgtatta
     1141 cctaaaagtc tcaaataaaa gttacatgtg tcagataaag tatttatctt taaggtgtta
     1201 aataaaaaat aacactcacc cgacacatgc aatacaaaaa tagtaaaaaa atctcattta
     1261 ctgccggttc agtaaagaaa gagttcagtt cagaacaatt aacgtcatat tcagggttaa
     1321 gcgtaacctc tgattttatc aaccattgtg gcatttatcg ccggctggaa catctttttc
     1381 caaccacccg gcacaatgca agccgtttca gtacagctca aatactctca agtatcctgt
     1441 tggcatcgtt gtgcggtgtt caccgtttga agcggattga aaatttcacc tttgacgcct
     1501 tggtttctcg cttgttgaag ttgcccaaaa acattgacca ggacaccatc cgccgccatt
     1561 tgacaggttt gggtgaaagg ggcgcccgtt cgcttcacga gctgttgttg ggttttacag
     1621 gcatgcaagt ttcccattgc ggtttaagcc gcttgacact tgattgcgac tcaagcacat
     1681 ttaccgttta cggcaaccaa caaggggctg aggtgggtta taactcgcat aagaagggtt
     1741 cgaaaagcta tcaccccatc ttatgttttg tcacggagat gaaactgctt gtcaattcat
     1801 ggctccgccc gggttcagct tacacctcaa acggagtttg tgagtttgtc aaagaaacct
     1861 tggccgctct tccccaaaag gtggagaagg tgtttttcag ggccgacagc ggttttttca
     1921 atggtggatt atttaatttg ttagaagacg gtaaacatga atatttggtg aaagtaaagc
     1981 tgaaaaacct gaaagattta cttgccgggc agacttggca gccgattggc ccacggacgg
     2041 cgacctgtca gtttacacat caatgtagcg gttggaggaa tccccgcatg ttttatgccg
     2101 tgcgtgtcgt aaagcaaatg gttgaagtcg attattttgg agagaaacaa tttgtacccg
     2161 agtatgagta cttttgctat tgctcgaacc tgaaaggatt ggatgccttg cagctccata
     2221 ccctttatgg atcccgatca gagagtgaga attggatcga gcaaaccaaa aactcgcttt
     2281 gtgcgggaaa aaccatcacg catgattttt gggtaaacga tattctttgg caactttcgt
     2341 catttgccta cagtttatcg gtgctcatgc gataccgggg cgacttttgg gtttggcgtc
     2401 aagagcactc taccttccga gaatggttta tccgagtgcc gggaaaagtg gtgaaatccg
     2461 gcaggcaggt cacggtaaaa atgcctaagg agtattaccg aaaagcgggg tggcgtgatt
     2521 ttgagcagcg aataacgaca acgatgaccg gatgacggat tttcacttaa tcgtccatcg
     2581 ccatctttcg tgtcgtaaaa gggtttatga caaggaattg ccatgccttg gaagagtggt
     2641 aatgttaata tatatataaa cgccaccata aaaagtaaat caacacgtta aacgtgcaag
     2701 aaacagtgaa gatgaaaaga aaataaaacg gtatctctcg aaaaaaaaga gaaattccgc
     2761 tttgaaaact attaacttga gttgggaatt ttaaatgaga tttttaagta ttagaatttg
     2821 attagtttct ttctattatt gcagcatctc ctcacagcac taatctgcag tgaaatcggg
     2881 tacaataact taactttttt tacatagtga gttgtataaa aagtttagaa tactaggtca
     2941 acctgcatgg ttgtaacgac tttagccata gttcataaaa ttcccaactc cattatttta
     3001 tcttcctcct tgaatgttaa ttttcagaat tttacctaac ccatcttttg attcggtagc
     3061 ccaaaagtaa tcagatggga aaagcccttc atctttcact aaacctgtaa gtttcataat
     3121 gattttgctc aactcttcca ttaatattgg attttcaggc gagcactcaa ataaaaaata
     3181 gtatttcgaa aaagagtcga agtttttaaa atcattttga tgcttcgctg caagctcaat
     3241 aatacgattg gttctatcag atagatcatt gttgccagag gcaatgataa tctgaatttg
     3301 ttccttgaat tggtagatat ctgccctatc cctatcagtc ataggtgttc tatataccgt
     3361 tccacatcgt gtctttgtat taaacggtgc tgtttccgga tataaccaat attcccattt
     3421 tttaaatgaa tcggtttcaa ttacttccgg ttgatgatag atgtattgtt tgttctcttc
     3481 tttattcatg ttgatgttca tcatgtgaat tgtggctttg tccataaaaa cctctttaga
     3541 accaatttca aagctgaatt attcaatact attaacataa aatcattatc aataatcttt
     3601 gtttgaattc tcatttcttt ttgcgttaaa ggaatgttat tctgactcaa agatatgaga
     3661 cagatgtgcc agatctaggc attgaatcgg ttataattca aaaagtatcg gatcgtttac
     3721 cagacgataa tcggcatcca ctacagctgc attggaaaag gtaatttctt tgtgtttttc
     3781 aatgccatag gcatcgtgaa tatgcccgaa cagatgatat gcaggacaga tccttgttac
     3841 tgcttgtaag agattagagc agccaaacct tgtgttgctt gactgatcct ggatcccgaa
     3901 cggaggccga tgtgtaataa gaacatcggt atcatccggt accaaatcaa atgctttggt
     3961 atctgacttg ggttcatccg gcgaaaagaa aaaaggtatt ccccagaaat ggatttcata
     4021 gattgtgatg ccactgttgt aaagatagaa acagttctcc gggagaaagc gctggattct
     4081 ttccggcgcc tttccttcca gcgcataatc gtggttgccg gcgatgaaaa tcttgtattg
     4141 atagcttaat gaaccaaacc attcaatgaa atccaacacc tcatcgcccg tgccggcaaa
     4201 cgtgatgtcg ccggagtgga tgatcacatc ggcagcaggc aaattgccca actgccgatg
     4261 ttgattgtga gtgtc
//
LOCUS       DAOSDD010000318         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7305_length_4265_cov_4.910451, whole genome shotgun sequence.
ACCESSION   DAOSDD010000318 DAOSDD010000000
VERSION     DAOSDD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7305_length_4265_cov_4.910451"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK733_18270"
     CDS             complement(<1..267)
                     /locus_tag="PK733_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12514.1"
                     /translation="MTVIKETTSDKIFDAINMVFVTLIMLIVLYPMLFILSSSFSNPD
                     YVSTGKVWLLPRGFNIEGYIRVFQDVEIMIGYRNTMFYTIAGTLL"
     gene            complement(289..1236)
                     /locus_tag="PK733_18275"
     CDS             complement(289..1236)
                     /locus_tag="PK733_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12515.1"
                     /translation="MSNSTKTSITFIDRKKTLWKRIAENYEMYIFLLPAVIYYIIFKY
                     GPLYGLQIAFKDYNGAMGIWGSEWVGLKHFKNFFSSYYFWPLIRNTLSISIYSLIAGF
                     PMPIILAIMLNEVKNYKYKKFVQTVTYAPHFISTVVLVGTIVIILSPTYGLVNQMLKF
                     FGMETRSFMTSPKLFRHIYVWSGIWQSTGWGSIIYLAALSSVDPEQHEAAIIDGASRI
                     QRIWYINIPVLIPIMTIIIIMNAGSIMNVGFEKVLLMQNDLIMDVADVISTYVYRRGL
                     IRAEFSFSAAIGLFNNVANFILLMIVNGITRKLGETSLF"
     gene            1464..3674
                     /locus_tag="PK733_18280"
     CDS             1464..3674
                     /locus_tag="PK733_18280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ12516.1"
                     /translation="MKYNLRQATIKYEYLTRLILTIFILVAIPFMISQYFITHRTYEE
                     IKVQNKQHYSESALHFCNYFKDQLSTVNDNAIKISVQKKLVQSKIFSNYWYRIEALEI
                     LETYREMIPNAENIAIHFKDTNIVLASDYTFHLDRFIERFSANDKEMKKDLQEIFSAQ
                     TDYSIKILTSFDTQMSNNAKLIIAIPIRMSFGIEKNATLMYIISNSSLSSNFFGLSSN
                     ESFGLAIFDNTKLIYANKDFDYELKTDNRFQDFICQKDDNFFTYKMNNVSKNIFKKYD
                     EDSGLAFISVVSENTVMQKVITFYGKIQTTMIILMLGIIFVSAFAVYISYKPISDLIK
                     NIQYHLPTTSVSSEIKTIRHTIDKMYSENNEMNEIVSEQRLLLMDYIFGNILYGFPIP
                     KNNKHLLYDFLEDKTFFVINIYDFSPNNLIREQLTQAAFEQLGISIFMLDALYGNYIT
                     IICVKNPCDSTEQLSTGFQALLEQTFSRKFVIGVGNAVDDINKIRISYLHALTELEHN
                     MKLILTRQIKNTTQDSSYTNDKIPQFLQCIQNGFESDALAQLSLIFDELINKEQYIFK
                     KNYLCYELISSYVNTMRNMNIIIEEHVFTDLIAFNSSEILQKKLEDNISIVCKIINQR
                     HETVDSHLKKLMIAYIDSSFTEPDITLVKIADEFNLSMYTCSRLFKEFTGTGFKEYIT
                     EKRMTLAKDLLLTTNKKVYEISNEIGFDNSTYFMTCFKANFGISPSKFRRQSNA"
     gene            complement(3745..>4265)
                     /locus_tag="PK733_18285"
     CDS             complement(3745..>4265)
                     /locus_tag="PK733_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12517.1"
                     /translation="VRKLAPQAQIVIHQTWAYRCDAEELKKYGISHEEMFACLKQAYS
                     EASKKLGCRIIPCGEAFQKARSIFNYTPDESYDFENAKPFELPEQSKSLIAGYFWKTG
                     NTVTGKAELRIDGKHGNAKGCYLANAVWYEMMTKNSILDNKFCPEGISQEELDILKKA
                     AHETVKEYGGSF"
BASE COUNT         1481 a          730 c          638 g         1416 t
ORIGIN      
        1 tagtaatgta ccagctattg tataaaacat cgtattccta tatccaatca taatttctac
       61 atcctggaac acccgtatat agccttctat attaaatcct cttgggagaa gccaaacctt
      121 gcctgtacta acgtaatcag ggttgctgaa tgacgatgat aatatgaata acatcggata
      181 taatacaata agcataatta atgtaacaaa taccatattt atagcatcaa atatcttatc
      241 gcttgttgtt tcttttatta cagtcattat gttatacctt tcctttcttt agaataagct
      301 ggtttcacca agtttacgtg ttataccatt cactatcatc aagagtatga aatttgctac
      361 attattaaat agtcctatcg ctgccgagaa actaaattca gcccgaatca agccgcggcg
      421 gtagacatat gttgaaatta catctgcaac atccattatg aggtcgtttt gcataagtaa
      481 tactttttca aaaccaacat tcataatgct tcctgcgttc attattataa taatcgtcat
      541 tatcggaata agaacaggta tatttatata ccaaatcctc tgaatccttg aagcaccatc
      601 tataattgca gcttcatgct gttcagggtc aactgaagat agagctgcaa ggtatataat
      661 tgatccccaa cctgtactct gccaaatacc tgaccacaca tatatgtgcc tgaacagctt
      721 cggacttgtc atgaagctcc gtgtttccat cccgaaaaac ttaagcatct gatttacaag
      781 accataagtc ggtgaaagaa tgattacaat tgtacctacg agaacaactg ttgaaataaa
      841 gtgtggcgca tatgtaactg tttgaacaaa cttcttatac ttataatttt tcacttcatt
      901 aagcataata gcaaggatta taggcatcgg gaaacctgca ataagggaat aaatagatat
      961 tgaaagtgta tttctgataa gcggccagaa ataatatgat gaaaagaaat ttttaaagtg
     1021 tttaagacca acccactcac ttccccatat acccatagcg ccattataat ctttaaatgc
     1081 tatctgcaat ccatacaatg gaccatattt aaagattatg taatatatta cagctggcaa
     1141 aaggaaaata tacatttcat aattttcggc aattcgtttc cataatgttt tttttctatc
     1201 tataaacgta atgcttgttt tagtagaatt actcatagtg ttttaccctc cttttccttc
     1261 gattttacat aatcaaaaat agtttgtaaa ctaaaatcat ttgctgttta cttagaaatt
     1321 cgcataattt tacgaaatgt tctgcatagt ttcagttttt gaagtaaaga gcaaaaattt
     1381 aaagtattgc tttatagtac attatgataa aataagttct gtaagaagag ctattttcat
     1441 atataaaatg gaggttggta gttttgaaat ataatcttag gcaagcaact ataaaatatg
     1501 aatacttgac aagacttata ttgacaatat ttatattggt cgcaattcca tttatgatat
     1561 cgcaatactt tattacacat agaacatatg aagaaattaa agttcaaaat aagcaacatt
     1621 actctgagtc tgcattgcac ttttgtaatt attttaaaga ccaactttca actgttaacg
     1681 ataatgctat taaaattagt gtccaaaaga aacttgtaca aagcaagata ttttccaact
     1741 attggtacag gatagaggct ttagaaatac ttgaaactta cagggagatg attccaaatg
     1801 ccgaaaacat tgcaattcat tttaaggata ccaatattgt tttggcatct gactatactt
     1861 ttcatctaga tcgctttatt gaacgttttt ctgctaatga taaagaaatg aaaaaagatt
     1921 tgcaggaaat atttagtgca cagacagatt atagcattaa aatattaaca tcatttgaca
     1981 ctcaaatgtc aaataatgca aagcttatta tagcaatacc cattaggatg agttttggca
     2041 ttgaaaaaaa tgcaacactt atgtatataa tctccaactc atcactttct tccaactttt
     2101 tcggactctc ctcaaatgaa agtttcgggt tggctatatt tgacaacact aagttaatat
     2161 atgccaacaa ggattttgac tacgagttaa aaacagataa tcgttttcag gattttatat
     2221 gtcaaaaaga tgataatttc tttacatata agatgaataa tgtaagtaaa aacattttta
     2281 aaaagtatga tgaggatagt ggcttagctt ttatttctgt tgtttctgaa aataccgtca
     2341 tgcaaaaagt aattacattt tacggcaaaa tacagacaac aatgattata ctgatgcttg
     2401 gaataatatt tgtttcagct tttgccgtat atataagcta taaaccaata tccgacttaa
     2461 taaaaaatat acaataccat ctccccacaa cttcagtaag cagtgaaatt aaaactatca
     2521 ggcacactat agataaaatg tatagtgaaa acaatgaaat gaatgaaatc gtgtcagaac
     2581 agcgactatt acttatggac tatatatttg ggaatattct ttatggtttt cccataccca
     2641 aaaataataa acacctttta tatgattttt tggaagataa aacttttttt gtaattaata
     2701 tatacgattt cagtcctaac aatctgatcc gtgaacaact aacacaagca gcttttgaac
     2761 aattaggcat atctattttt atgctagatg ctttgtatgg caattacata acaataattt
     2821 gtgtaaaaaa cccctgcgac agtacagaac aactgagtac cggttttcaa gcacttttgg
     2881 aacagacatt tagcagaaaa tttgttatag gtgtaggtaa tgcagttgat gatataaata
     2941 aaatacgaat ttcttatctt catgctctaa cagagctgga acacaatatg aagctaatac
     3001 ttaccaggca gataaaaaat acaactcaag attcttccta taccaatgat aaaatacctc
     3061 aatttttgca gtgtatccaa aatggatttg aaagtgacgc tctagcacag ctttcattga
     3121 tttttgatga gcttattaat aaggaacagt atatttttaa aaaaaattat ttatgctatg
     3181 aacttatcag ctcatatgtt aatactatga gaaacatgaa tattataatt gaggagcatg
     3241 tttttactga cttgatagca tttaattcct cagaaatcct ccaaaaaaag cttgaggata
     3301 atatatcaat tgtctgcaaa ataataaacc aaagacatga aacagttgat agtcatctga
     3361 aaaagctgat gattgcatat atagattcta gttttacaga acctgacatt acactcgtta
     3421 aaattgctga tgagttcaat ttgtctatgt atacatgtag tcgtcttttt aaagaattta
     3481 caggaactgg ttttaaggaa tatataaccg aaaaacgtat gacacttgca aaagatttgc
     3541 tgctaacaac taacaaaaaa gtttatgaaa tatctaatga aatcggattt gataattcca
     3601 catattttat gacatgtttt aaggccaatt ttggcatatc tccgagcaag ttccgaagac
     3661 aaagcaatgc ataattaatt tagcctgttg tgtttggtgc cgggcaccaa acacaacagg
     3721 ctaaatactc aaattagaat ataatcaaaa actaccccca tattccttca ctgtttcatg
     3781 agctgctttt ttcaggatat ccagttcttc ttgtgaaatg ccttctggac agaatttgtt
     3841 gtcaagtata ctgttttttg tcatcatttc ataccataca gcattggcaa ggtaacagcc
     3901 ttttgcattg ccatgcttgc catcaatgcg tagttcagcc tttcctgtaa cagtatttcc
     3961 cgttttccaa aaatagcctg ctattaatga tttgctttgc tcaggcaatt caaatggttt
     4021 tgcattttca aaatcgtaac tctcatccgg tgtataatta aatattgaac gtgctttttg
     4081 aaatgcttct ccacatggta ttatcctgca tcccaatttc tttgaagctt cagaataggc
     4141 ctgcttcaaa caagcaaaca tttcttcatg gctaattcca tattttttaa gttcttcagc
     4201 atcacaccgg tacgcccaag tttgatgaat tactatctgt gcttgtggtg caagctttct
     4261 aacca
//
LOCUS       DAOSDD010000319         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7341_length_4249_cov_5.727945, whole genome shotgun sequence.
ACCESSION   DAOSDD010000319 DAOSDD010000000
VERSION     DAOSDD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7341_length_4249_cov_5.727945"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..569
                     /locus_tag="PK733_18290"
     CDS             192..569
                     /locus_tag="PK733_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HOJ12518.1"
                     /translation="MEGQSSLKKQLRLLEELLLQPEIRKSVKEIDALLADDFIEYGSS
                     GRIYNKKIELDEMPKAPNVQTTITDFEVKLLADGVALATYCAVRHGNEGEELRYSLRS
                     SIWRLHKGKWQVVFHQGTPSIAP"
     gene            980..1795
                     /locus_tag="PK733_18295"
     CDS             980..1795
                     /locus_tag="PK733_18295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ12519.1"
                     /translation="MEDTSIKMVKQYYDDTAEKEWDRLDSHPFEFVLTTWMMDKYIKS
                     GDTILDIGGGPGRYSIYYAQNGCSVTLVDLSDSNISLAEQKAKQFGVSIKSYAKNCLE
                     LDELNLEQFDHVFLMGPLYHLQKENDRINAVKAALRHLKKDGKLYVSFIMAFAGIIYD
                     LQHAGYIVGDANNPATAPLLESVAHGNDYLGPGFTAVYFYNQNNILSFMQRFTIEKLH
                     LFGQESFLAPNKLDIIAREHSEINCWIELAKRYIELPELLSWSEHAMYIGNKL"
     gene            1987..2421
                     /locus_tag="PK733_18300"
     CDS             1987..2421
                     /locus_tag="PK733_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease"
                     /protein_id="HOJ12520.1"
                     /translation="MQIVFKNCNDKKYEHMLNNLLKNIFFDFQFWYELNLWDDKYESY
                     SIIENDELVSKISQDCVDDINIRFTGKEEIVTTFGIGGKEHAFIKKIDKVILGDLIIE
                     KHEMDFTGYIYDDINGLLGLDILIKGGFIIDLNDLAIYRNKR"
     gene            3070..3849
                     /locus_tag="PK733_18305"
     CDS             3070..3849
                     /locus_tag="PK733_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12521.1"
                     /translation="MRNNMISGQNNIQKSFQSLFGKEELFTKVIECFPYPVQIFSVDG
                     TARLINKATLEMIGIRSIESHVGKYNVFKDPIVQELGFMDQVKQVLTGKTVYLTDFNA
                     PYQDMIRYFNVEDRDIQTISSDITCFPLVNADGVVEYFAAVFIFKKIYRGKKEIGWGR
                     QYIETHWREPFDADKTAKAASLSKAHFTKLFKKHTGVTPHDYYINYKISKLKQKLLDT
                     NLSIAQAFAACNMNYNGHSARLFREKVGVPPSVYRKMSEVK"
     gene            complement(3966..>4249)
                     /locus_tag="PK733_18310"
     CDS             complement(3966..>4249)
                     /locus_tag="PK733_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12522.1"
                     /translation="FCKVYYSHNNQQPVFTCLVMFAHSINIITPWVIIASSVGTGKIT
                     ALLKQSGCGTLCRVTPENMCKSLVHQCSFPSGLLIFYIAAINILSTGWL"
BASE COUNT         1409 a          694 c          939 g         1207 t
ORIGIN      
        1 gacaaaggta gccagcacca cacgtcctca gcaagataag agctatctaa gcttcaggac
       61 gcgtcttgag tgctgaccgt tagctgaaat gcagtccatt tatacgcccg gacctccggt
      121 tgtcggacgc aatagtacaa aaagtggggt aagtcacccg agtgtattac atagggaaag
      181 ggatctaaag tatggaagga caatcttcat taaagaaaca acttcgacta ttagaggaat
      241 tattactgca acctgaaatt cgtaaatctg ttaaagaaat agatgcttta ttagcagatg
      301 actttataga atacggtagt tcaggtcgca tatataacaa gaaaatagaa cttgacgaaa
      361 tgccaaaagc accgaatgtc caaacgacaa taactgattt tgaagtgaag ctattagccg
      421 atggcgttgc gctggcaact tactgtgcag tcaggcatgg taatgaaggt gaagaactaa
      481 gatattcatt gcgtagctcc atctggagac tgcataaagg taaatggcag gtagtatttc
      541 atcagggtac accatctata gcaccttgat attgcctaaa acaaacgctc tgttcggaaa
      601 cagacgtgat tatacttaag aacaaggaaa tgatgtatac gaaatgataa tgaaacaaga
      661 ttgctgagta atgtctttgt attatgccac agccttgccc tccgggcgat cggctgtgga
      721 gggctgcaaa tcagctaaca atgcactcaa gtgaaggtta ctggcttata ttgtaaatgg
      781 taaaattgaa tcagttaggc aaaggtaacc agcaccacac gtcctcagca agataagagc
      841 tatctaagct tcggatgcat cttgagtgct agccgttata tgaaatatcg ctcatcggac
      901 gtcgcccggg cctccggccg acgggcgaat ctgcattaca taatacacta ttgaggccag
      961 tatagacagg aggttatcta tggaggatac atctattaaa atggttaaac agtattatga
     1021 tgataccgct gaaaaagaat gggatcggtt ggattcgcat ccctttgagt ttgtattaac
     1081 aacttggatg atggacaagt atataaaatc tggagacacc attcttgata ttggtggggg
     1141 tcctgggcgt tactctatat actatgctca aaatggatgc tctgtaactc tagttgattt
     1201 atcagacagc aatattagtt tggcagagca gaaagccaaa caatttggtg tttcaattaa
     1261 atcttatgct aaaaactgtc tagaattgga tgaactgaat ttggaacaat ttgatcatgt
     1321 attccttatg ggtccgttat atcatctgca aaaggaaaat gatcggatta atgcagttaa
     1381 agcagcactg aggcatttaa aaaaagatgg caagttgtat gtttctttca ttatggcttt
     1441 tgctgggatt atatacgatt tacagcatgc aggatatatt gttggtgatg caaataatcc
     1501 ggcaacagct ccacttttag agtctgtggc gcatggtaat gactatttag gacccggctt
     1561 tacagcagta tatttctaca accaaaataa tattctgtct ttcatgcaac ggttcactat
     1621 agaaaaacta catctattcg ggcaggagag ttttttagcg cccaataaac tagatattat
     1681 tgctcgtgag cactcagaga tcaattgctg gattgaattg gccaaacgat atattgaatt
     1741 gccagaactg ttaagctggt ctgaacatgc gatgtatatt gggaataagt tgtaaatatt
     1801 ttatttgtca tgagaatatg gacattctat ttgtttcgca ttctttatat gttggatgag
     1861 tgtaaagaat tagaatgtaa tccggccttg actccgccgt gcggccgggg aaggtaagcc
     1921 gcatcccgca gtgaattctc tgaatggaca atgatgtaaa acaaatcaat taaggatggt
     1981 atgattatgc aaattgtatt taaaaattgt aatgataaaa agtatgaaca tatgctaaat
     2041 aacttattaa agaatatttt cttcgatttt cagttttggt atgaattaaa tttgtgggat
     2101 gataagtatg agagttactc aattattgaa aatgatgagc ttgtatctaa aatttctcaa
     2161 gattgtgtag acgatattaa tattagattt accggaaaag aagaaatagt aacgactttt
     2221 ggcattggtg ggaaagaaca tgcctttatc aagaaaattg acaaggtaat attaggagat
     2281 ttaataatag agaaacatga gatggacttt acaggatata tttatgatga tattaatgga
     2341 ttattaggat tagacatatt aataaaaggt ggatttataa ttgacttgaa tgatttagct
     2401 atctatagga acaaaagata gcttttattt ttatatgcat agatgaaagt gacaaaatgt
     2461 agggaaaaaa cttcacataa cagtgtattt gcgcaagggt gcacccggac acaatcttag
     2521 ggtgatgtgc accacatcct ttcaccggga gtgaagttcc gccagggggg tctatctctt
     2581 gggtccggtt cgtcggcgtc gtaaaaccgg gaccgttagg cgacagaaca cgtttatagc
     2641 gtatctttag ggtctgtttt taatgaaact aggaaattta gataagaatc tgctattcta
     2701 gtttggtata ataaagttat ggtatgaaaa acaaaagggg agattttagg acttgggaag
     2761 aagcaagaaa ttaatatcca aacaggtttg ttaagttcaa gataatagaa tcacatattg
     2821 aagaaaataa ggagtatgtt aatgaagttg ctgttataaa agctaaagaa gatgggaaag
     2881 aagccatgaa agaatttatt aggtgtaaaa gaaggggagt acatttatag aactaaagcg
     2941 gctgctttgc gatttgataa aaccggcaaa tgcagtaata ttctatgacg aaagatccat
     3001 ctaaacattg aaatatactg agtgacgtag aaaagcctca agatggcagg tataaaaagg
     3061 tgataaaaag tgaggaataa tatgatcagc gggcaaaaca acattcaaaa gtcatttcaa
     3121 tcgctctttg gaaaggaaga actttttacc aaagtgatcg agtgtttccc ctatccggtt
     3181 cagattttct ctgtcgacgg gacagcgagg ttgattaaca aagcgacgct ggaaatgata
     3241 ggaatcagaa gcattgaatc ccatgtgggc aaatacaatg tttttaagga ccccatcgtg
     3301 caagagcttg gctttatgga tcaggtcaag caggtgttga caggtaaaac cgtatatctt
     3361 accgatttca atgcgcctta tcaggatatg atacggtatt ttaatgtgga agatagagat
     3421 atacagacca taagctcaga cattacatgt ttccctttgg taaacgctga tggtgtggta
     3481 gaatactttg ctgccgtttt tatctttaaa aaaatatacc gggggaaaaa agaaattgga
     3541 tggggcagac aatacataga aacgcattgg cgggaacctt ttgacgccga caaaactgca
     3601 aaggcggcaa gcctcagtaa ggcacatttc acaaaattgt tcaagaagca taccggcgta
     3661 acaccgcatg actattatat taattataaa atcagcaaat tgaagcagaa gctgctggat
     3721 accaaccttt ccatcgcaca ggcttttgct gcttgcaata tgaactataa cggccattcc
     3781 gcaagattat ttagagagaa ggttggcgtt cccccgtctg tatatcggaa aatgtcagag
     3841 gtaaaatgaa ttgtcctatt aagcaataag agggagccgg actttggttt agcacaaaag
     3901 cccggcttct ttttaatttc aaaataatat tttttgtact aattataggc atattcaacc
     3961 tatccttaca gccagcctgt tgataaaata ttaatagcgg ctatgtagaa tatcagcagc
     4021 ccggatggaa aggagcattg atgaacaagg cttttgcaca tgttttcagg agttacacga
     4081 caaagtgtac cgcatcctga ctgtttcaat aaggctgtta tttttcctgt tccgaccgat
     4141 gatgctataa tcacccacgg tgtgattatg tttatgctat gagcaaacat gacaagacaa
     4201 gtaaacacgg gctgctggtt attgtgtgaa taataaacct tacaaaaac
//
LOCUS       DAOSDD010000320         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7355_length_4244_cov_5.038195, whole genome shotgun sequence.
ACCESSION   DAOSDD010000320 DAOSDD010000000
VERSION     DAOSDD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7355_length_4244_cov_5.038195"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK733_18315"
     CDS             <1..191
                     /locus_tag="PK733_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ12523.1"
                     /translation="GIEIALTRYAKKHNIRVREVKLDNMTHITKEEKYGFTAGFRKRL
                     TMYKQIFKGLKLAKGDER"
     gene            585..>4244
                     /locus_tag="PK733_18320"
     CDS             585..>4244
                     /locus_tag="PK733_18320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOJ12524.1"
                     /translation="MQNVALNDSFSKIFSKELAIGHDTEGLPGNVKVQTINYYKRSKL
                     LEIKMKSDKLIPAQVLADIEDRFAGYFNIDSVEVKVKYIISESIEALLNSYWESIVFS
                     TCRKLATSRGLLSGCKWSLSERKLTVYLKTKGADILKAQGCAKHMETLIEEAFSTRIK
                     VEFIDCIIDENYKKKYIEKKKTEEQKVLNNIIIMSADNKSNSIYKNATGSNQASVADK
                     NETQSSIIFGKDFNDSLLKMKEINQDSGKVSIGGDILKVEFREIRGERFICTFDITDN
                     TNSLTVKFFVKKDKIDGVKNRIKEGITVKVRGEAQYDKFSRDVAIMASDIVLMGKKQK
                     RMDTAKVKRVELHLHTQMSAMDGVTSVEALVKRASEWGHKAIAITDHGVIQAYPGASA
                     MAGKSNIKIIYGVECYLVDDTADENCDYTKAHAYHAIILVQNNKGLKNLYKLISKSHL
                     EYYYKKPRMFKSMILQYREGLILGTACEAGELYNAVLDNKSEEEINRIVSFYDYLEIQ
                     PLGNNQFLVENGRVKNREELKKINMEIVKLGEKLQKPVVATCDVHFLDPEDEVYRRIL
                     MAGQGYSDADKQAPLYLRTTDEMLAEFEYLGEEKAYEVVISNPNKINDMLETIAPVPK
                     GTFPPQIEGAEYEIKELSINHAKEKYGDPLPEIVEDRLEKELESIIKNGFSVMYLIAI
                     KLVRKSLSDGYLVGSRGSVGSSFVAYLTGITEVNPLHAHYVCNKCKYSEFITDGSIDC
                     GYDLSGKSCPQCGENLKRDGYDIPFETFLGFNGDKEPDIDLNFSGQYQPTAHKFVEEL
                     FGEGHVFRAGTIASVAEKTAYGFAKNYLDERGIVTTTAELNRLIKGCTGIKRTTGQHP
                     GGVMIIPQDKEIYDFTPIQHPADDTGSNTITTHFDYNFLHGSILKLDILGHDDPTTIR
                     MLEDLTGIDARTIPIGDPETMKLFSSTSSLGVTPEDVNSEVGTLGIPEFGTRFVRQML
                     VETRPKTMSELIRISGLSHGTDVWINNAQDLIKANIASLSEVICTRDDIMLYLLYAGL
                     PPITSFKIMEDVRKGKGLKPEYEDIMKANNVPAWYIDSCKKIKYMFPKAHAAAYVMMA
                     FRIAWFKVNYPVEFYTTYFTVKADEFDAEVMTQNHDIIKEKIMELEDKGNNVTQKDKS
                     ILTLLEVVNEMYSRGINFIPVDLYKSEAKKFTIDGGSIRPPLNGLPGLGITAAQNIVE
                     ARKEEKFSSIEDLRIR"
BASE COUNT         1543 a          647 c          901 g         1153 t
ORIGIN      
        1 atggaattga aatagcttta accaggtatg caaaaaagca taatataagg gtcagggaag
       61 taaaacttga taacatgacc cacataacca aggaggaaaa atacggattt accgccggtt
      121 tccgtaaaag gctgacaatg tacaagcaga tttttaaagg gttaaaattg gcaaaaggcg
      181 atgagcgata agcaatttta aatatatata acgcaagggt gcattaaaat ttttgaagaa
      241 atatattcca gaagctaccg ttacagtact tagagccttt aaattcagct acgggcaaaa
      301 tcaaactcac aaaataattc gaataaaaaa attgtaatta ttttgttcaa acactgattt
      361 tgctcctcct tcgctccgat taaaaggctc tacagtacct tcactaatgc ttcttccaat
      421 aatttcttca aaaataaaat atgcacccat agcgcaaaaa tgatttgaca agaaacaaat
      481 tagatttttg cgtattaatt gagataacat aagcgcacaa aattatataa tatagtatat
      541 tttgtgcgtt atctatagaa cacttaagat aaggggtatg aattgtgcag aatgttgctt
      601 taaatgacag cttttcaaaa attttttcta aagagttagc aataggtcat gatactgaag
      661 gtttacctgg taatgtgaaa gtacaaacta ttaattatta taaaaggtca aaattactag
      721 aaattaaaat gaagtcagat aaattgattc cagcacaggt gctggctgat atagaagata
      781 gattcgctgg atactttaat attgattctg tagaagtaaa agtaaaatat attatatcgg
      841 aatctatcga agctcttctt aactcttatt gggaaagtat tgttttcagt acttgcagaa
      901 aattagccac aagtcgtgga ctgctttcag gctgcaaatg gagtttatca gaacgtaaac
      961 tgactgttta ccttaaaacc aaaggtgcag atatactaaa agctcaggga tgtgcaaagc
     1021 acatggaaac tttgattgaa gaagcctttt ccacaagaat taaagtagag tttattgatt
     1081 gtataattga tgaaaactac aaaaaaaagt atattgaaaa gaaaaagacg gaagaacaaa
     1141 aagtattaaa taacattatc attatgtctg ctgataataa aagcaattcg atatataaaa
     1201 atgcaacagg cagtaaccag gcttcagtag ccgataaaaa tgaaacccaa tcttcaatta
     1261 tttttggaaa agattttaat gattctcttt taaaaatgaa ggaaatcaac caggattcag
     1321 gtaaagtttc tattggagga gacatcctga aggttgaatt cagggaaata aggggagaaa
     1381 ggtttatatg tacatttgac ataaccgata ataccaattc acttactgtt aaattctttg
     1441 taaaaaagga caagatagat ggggttaaaa atagaataaa agaaggaata acagtaaagg
     1501 tgcgtggaga ggcacagtat gacaagttct caagagatgt agcaataatg gcatcagata
     1561 ttgttctgat gggaaaaaaa caaaagcgca tggatactgc aaaggtgaaa agggttgaac
     1621 tgcatttaca tactcagatg agtgcaatgg atggggttac atcggtggaa gcactggtaa
     1681 aaagagcttc agaatggggt cataaagcta ttgcaataac tgaccatgga gtaattcagg
     1741 catatcccgg tgcatccgca atggccggga aaagcaatat aaaaatcata tacggagttg
     1801 agtgttattt agtggatgat acagcggatg aaaactgtga ttatacaaaa gctcatgcat
     1861 atcatgcaat aatacttgtg cagaataata agggcttgaa gaatctttac aagcttatat
     1921 caaaatctca cctggaatat tactataaaa aaccaagaat gtttaaaagc atgatattgc
     1981 agtatagaga aggcttaata cttgggacgg catgtgaagc aggcgagtta tataacgctg
     2041 tactggataa caagagtgaa gaagagataa acagaattgt ttctttttat gattatcttg
     2101 agatacagcc attgggcaat aatcagtttt tggtagaaaa cggacgagta aaaaacaggg
     2161 aagagcttaa aaagataaac atggaaattg taaagctcgg agaaaagctt caaaaacctg
     2221 tagttgcaac ctgtgatgtg cattttttgg atccggagga tgaagtgtac aggcggatat
     2281 taatggcagg tcagggatat agtgatgcgg ataaacaggc accactgtat ttaaggacta
     2341 cagacgagat gcttgcagag tttgagtacc ttggggaaga aaaggcttat gaagtggtaa
     2401 tatcaaatcc caataagatt aatgacatgc ttgaaactat tgcaccagtt ccgaaaggaa
     2461 cttttccacc ccaaatagag ggtgctgaat atgaaattaa agaactctca attaatcatg
     2521 caaaggaaaa gtacggtgat cctttacctg aaatagttga ggacaggctt gaaaaagaac
     2581 tggagtcaat aattaaaaat gggttttccg taatgtacct gatagcaata aaactggtgc
     2641 gtaaatcatt aagtgacgga tatctggtag gctcaagggg ctcagtaggc tcctcgtttg
     2701 tggcatatct tacaggaatc acggaagtta acccattgca tgctcattat gtatgcaata
     2761 aatgcaagta ttcagaattt ataactgatg gtagtataga ctgtgggtat gacctttcag
     2821 ggaaaagctg ccctcaatgt ggagaaaatc ttaaaagaga cggatatgat attccatttg
     2881 aaactttcct ggggtttaac ggagataaag agccggatat cgacctaaac ttttcaggtc
     2941 aataccagcc tactgcacac aaatttgtag aagaactatt tggagaagga catgttttca
     3001 gggcaggtac cattgcctct gtggccgaga agactgctta cgggtttgcg aaaaattacc
     3061 ttgacgaaag gggtattgtt actacaactg cagaattgaa ccgccttata aaaggctgca
     3121 cagggattaa aagaactacg ggccagcatc ctggcggtgt aatgataata cctcaggata
     3181 aggaaatata tgattttact cccatacagc atcctgcgga tgatacaggg tcaaacacta
     3241 taacaacaca ttttgattat aacttcctcc atggcagtat attaaaactg gacattttag
     3301 gacatgatga tcctaccacc ataagaatgc tggaagacct aacagggatt gatgccagaa
     3361 caattcccat cggtgatcct gaaacaatga aactattcag tagtacaagt tcattggggg
     3421 ttacaccgga agatgttaac agtgaagtag gaactttggg catacctgag tttggaacca
     3481 ggtttgtaag gcagatgctt gtagaaacaa ggcctaaaac catgtctgaa cttattagaa
     3541 tatcaggttt gtctcatggc actgatgtat ggatcaataa tgcccaggat ttaataaagg
     3601 caaacattgc cagcctttca gaggttattt gtacaagaga cgatatcatg ctttatcttc
     3661 tatatgctgg gctgccgcct ataacttcat tcaaaattat ggaagatgta agaaaaggga
     3721 aaggcttgaa acctgaatat gaagatatta tgaaagctaa taacgtaccc gcatggtata
     3781 tagattcatg taaaaaaata aagtatatgt ttcctaaagc tcatgctgcg gcatatgtta
     3841 tgatggcctt caggatagcc tggttcaaag ttaattatcc tgttgaattc tatacaacat
     3901 attttactgt aaaagctgat gaatttgatg ccgaagtaat gactcagaat catgacatta
     3961 taaaagaaaa aataatggaa cttgaggata aaggcaataa tgtgacacag aaggataaaa
     4021 gcatcctaac cttattggaa gttgtaaatg aaatgtattc aagggggata aactttattc
     4081 ctgttgattt atataaatcg gaagctaaga agttcactat agatggcggg agtatcagac
     4141 cgccccttaa tggattgccg gggcttggaa taacggctgc acagaacatt gttgaggcca
     4201 ggaaggaaga aaagttcagc tctattgaag acctgcgaat aagg
//
LOCUS       DAOSDD010000321         4212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7418_length_4212_cov_5.542218, whole genome shotgun sequence.
ACCESSION   DAOSDD010000321 DAOSDD010000000
VERSION     DAOSDD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_4212_cov_5.542218"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1562..2233
                     /locus_tag="PK733_18325"
     CDS             1562..2233
                     /locus_tag="PK733_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12525.1"
                     /translation="MSSNDNFTKQLKNLIAEFVTPSGEILEYPTGRYYGRKQLDSRRL
                     KLLRIFTEILPRTRFMRQETKTYISDKYITLKGVAEHMQSIGIEINANTVQSNIWMDM
                     ERVDAKLSKRILLDIVEYNTGFDEYEQRLLKTLAHYSNGHLFRNLTLKLPEPIMKIDA
                     TEEEFCDFVKCIAPYSPQHMAYLRDNMSSEMLGYSTYIISSTLLTEEEQERKDFLLQL
                     LYKET"
     gene            3281..>4212
                     /locus_tag="PK733_18330"
     CDS             3281..>4212
                     /locus_tag="PK733_18330"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HOJ12526.1"
                     /translation="MSTGNIVQNEADKIRLATGKQLLTLEEAANYAGIGTGDFAEIIS
                     SGDNPIKLLGKGYIRIADLTSFLYGGLGMNEVEKIVEKPLDSGFPQRYPPSKIEDLSE
                     EGYMEMERRGKGEGSIFYNESRKVWQAAVSLGYDEKGKRRRKIISGKSEEEVGEALRL
                     ILAAYTPTAAEQKNIAETVPELGAVSTEKAKDITFKEFLDNCLKNIKGRPQSRTFFNY
                     IGTAKHLEEGLGHILISQLTKDDCERFINGFTEKTYARGKTVSKYGHSMIHKIYIMLK
                     MVLREAVDRELIRKNFVDSIKEPRSKKYEEPKYKA"
BASE COUNT         1451 a          629 c          886 g         1246 t
ORIGIN      
        1 ctgatcttca acccactgtc tgtgtttaac tcttgtaggt ttctttaact taacaacaaa
       61 agttatagtt gccaataatt tcacctcctt tgtcaaacag atgttcttta attaaatagt
      121 accataattt gcttgtatat gcaagagaaa acttcaatct ttttcaaaaa ctctttctgg
      181 actttttgat atttgctttg gttttttctt tcttataata gattagcagc gatagatttg
      241 ggaaataagg tagtcatgca ttcatctccc atttaaaaaa gggagcgttc tgcgtggtct
      301 acgtaaattt ttataaaaaa cttataaaaa tggttttgag aatttgagtt tacgggatgc
      361 attcatatgt agtcgaatgg acattattat tattaagtca ggtatcccct acagtgctaa
      421 tttgcaggac agttcgccta ctcttgttcc ataaccaaca actcaataaa tttagatacc
      481 ttctaaaatt aaggagagtg tcccgaattt tgtgttttct tgttatctca gtagttaccg
      541 cagttaactt aatagttgtc aagatggcat atacatcaag ctggagcgaa aaataagtgt
      601 tatgtatatt atgggttttg tatgaagaac tgcagtttca aagcggcgaa acaaataatt
      661 gctttcgacg ttctgacatt aatcttcagt aagccgtggt atgttcattt tcttcagttt
      721 ttttgaataa gcaagtgtga cgtatatttt ttaaatttgc aaatgtgaga attgatttta
      781 ttaatgtatc gtttattacg taaacgcata taatattata cagggtggta agagcgctga
      841 atttccttgt aaatcattaa aatcaccttg acatatgtga atgtgatgtt ataattaaag
      901 catgaaacaa acgtgcttca caatgtgcac cgcagaaaca ttgttgatac aaaaacgctt
      961 cacgatgtgc gacgcaggaa catcgttgat acaaaaacgc ttcacaatgt gcgacaaaag
     1021 aacattgtag aaaagtccaa gtgaaagctt tggacttttc tttttgagga caaaagggga
     1081 caagatgaga gagaatggaa tgtattttgg gacgggggac atctaccaaa aaataagaga
     1141 tgttggtgga gaatggaatg acgctaaaga gcggccgatt gtatgctaaa tcgagtccac
     1201 agaaaggtct ggtctttatt gggcaatacc tgttgggaac tggaatcaca gagacgccgt
     1261 gactcagcaa agaattacta agttcatgtc ttataacaat agtgatttaa ggtcatgtta
     1321 ttaccataca ggaaaaacga ctactcaatc aatcttcttt attagtgatg ttattcctat
     1381 tacagacaaa tatattgaaa gagaatatga aggttatgac aaaaaaatct acataagctg
     1441 ttaaagtcga gcctgatatt gcagaaaacc ttgtgcatgt cagtgcaatt cctggtttgg
     1501 atgcatactc cattgaaatc gtttatcagg tagaaaaaga ggatgaggag gctgatgtat
     1561 tttgagcagc aatgataatt tcactaaaca attaaaaaat cttattgctg aatttgttac
     1621 tccatccggt gagatattag aatacccgac ggggcgatat tatggtagaa aacagctgga
     1681 tagccggaga ttaaagcttc tgcgtatttt cactgaaata ctcccgcgaa caaggttcat
     1741 gagacaagaa actaaaacct atataagcga caaatatatc actttaaaag gagtagcaga
     1801 gcatatgcag tccataggca tagagataaa tgccaatacg gtacaatcaa atatttggat
     1861 ggatatggaa cgtgtagatg ctaaactgtc aaaacgtatt ttgctggata ttgttgaata
     1921 taacacagga tttgatgaat atgagcagcg gttactcaaa acactggcac attattcgaa
     1981 tggacacctg ttcagaaatt taacattaaa attacctgaa cccattatga aaatagatgc
     2041 caccgaagag gaattttgtg actttgtaaa atgcatagct ccctacagcc cacagcacat
     2101 ggcataccta agggataata tgtcttctga aatgttaggc tattcgacgt atattattag
     2161 cagtacgcta cttacagagg aagaacagga aagaaaggat tttctgttgc agctccttta
     2221 taaagaaaca tagtttatca gatgaaacaa agtatacaat gaaattctat ataagcccat
     2281 aaagataaca gagtaaagct gtgacagcct gctctgttat ttttttgtat ttaagatagt
     2341 ggttagggca gtgattaaac tccggcaatc tgagcaaatt gcgtgaggat gtatgatagt
     2401 agaataaaaa gtgatatttg ggaagcagta aggcgacttg ttggataaat actgagctat
     2461 aaggaatggg gcaatacatt cttgatgcag gttccatgtg atatgtaata tttcaataat
     2521 tccggtatat ttttagtact gaaattaggc tggttgagat ggcgttgctc cgatacaaga
     2581 gcgaggaaga atatgccatc attaccagtt ttgtttatat aacctatatt tttagtgttt
     2641 atgtcctgcg gtaggtacag attgaaactt ataactatca aaaaccttaa tgaattataa
     2701 ccggaatttg acaatttata atagtgaaat gtaaccaagg aacgaagtct gagtttagta
     2761 agtaattttc ttatgttgaa ataggtccaa aatagcggtt ccagagaaat atattggtta
     2821 ggtagggata taaaattagg tgttgagaaa ttagggttaa aattgatttt acaaagaggt
     2881 agcggacaaa aatttatcgt ttgcatggac gttatatgaa tggagtattt agtcatctca
     2941 caggtaaaaa gatgttaagt ttataaatcc atgatctttc cctgctgttg ataaaagttt
     3001 ataaaatata aaaattattg gctgtcaaag agctggttaa atttggtgtt atgtggaaat
     3061 ctaggttgtg ataatgattt ttatatctct atcttgactt atgtaaactt tgctatacat
     3121 ggacaattct tttatggggc atagaattag gttacataca ttcaaaaaag tattgacctt
     3181 attgtagaat tctgacatgg acagtgcatt aaaactatca gagctttaca aacatagtaa
     3241 aaatattagt atgcatcttg attataagga gatgcatatt atgtcaactg gaaacatcgt
     3301 ccagaacgaa gctgacaaaa tcaggcttgc tactggcaag caactgctta cattggagga
     3361 agcagcaaac tacgccggca ttggcaccgg agattttgct gagataatca gcagtggtga
     3421 caaccccata aagcttttag gtaaaggtta tatcaggata gccgatttga cgagcttctt
     3481 gtatggtgga ttagggatga atgaagtaga aaaaatagtt gaaaaacctc ttgactcagg
     3541 cttcccccag cgttaccctc ccagcaaaat tgaagatttg agtgaggagg gttatatgga
     3601 aatggaaaga agaggaaagg gcgaaggctc tatattttac aatgagagcc gaaaagtgtg
     3661 gcaggcggct gttagtctag gctatgacga aaaaggaaaa aggcgtagga aaatcataag
     3721 tggtaaaagt gaagaagaag tgggagaagc cttgcggttg atactagctg catacacacc
     3781 tactgcagca gaacaaaaga acattgcaga aacagtgcct gaacttggtg cagtgtctac
     3841 agaaaaggca aaggatatta cttttaaaga atttctggac aattgcttaa agaatatcaa
     3901 gggcagacca cagtcgagaa catttttcaa ttacatagga acagcaaaac accttgaaga
     3961 aggattaggg catatattaa tatctcagct cacaaaagat gactgcgaga ggtttataaa
     4021 cggcttcact gaaaagacat atgcaagggg gaaaacagtt agcaagtacg gtcatagtat
     4081 gatacacaaa atatatatca tgctcaaaat ggttttgaga gaagctgtgg atagagagtt
     4141 gataaggaaa aactttgtgg attcgataaa agaacctagg tctaaaaagt acgaagagcc
     4201 taagtacaag gc
//
LOCUS       DAOSDD010000322         4189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7483_length_4189_cov_4.246009, whole genome shotgun sequence.
ACCESSION   DAOSDD010000322 DAOSDD010000000
VERSION     DAOSDD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7483_length_4189_cov_4.246009"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..2022)
                     /gene="aspS"
                     /locus_tag="PK733_18335"
     CDS             complement(244..2022)
                     /gene="aspS"
                     /locus_tag="PK733_18335"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOJ12527.1"
                     /translation="MEESIYGMKRTYMCNDLRPDNVGKTVTVMGWVHKRRDLGGVIFI
                     DLRDRSGILQVVFRLDQNSEIFRKAEGLRSEYVIAVVGEVVKRDPETVNPKIDTGEIE
                     VAVRELRILSQAETPPIYIEEDSDANELLRLKYRYLDLRRPDMQKNFILRHRVAKIAR
                     DYYDSKGFLEVETPMLTKSTPEGARDYLVPSRVHPGKFYALPQSPQLFKQLLMVSGFD
                     RYFQIVRCFRDEDLRADRQPEFTQIDIEMSFVNVEDVIEINEGFIKKAFKEALGVEFT
                     EPFIRMPYQEAMDRFGSDKPDIRFGFELVNISDIVENSGFKVFSDAVKNGGSVRLINA
                     KGCGEKFSRREIDSLVEFVKTYKAKGMAWIVVEENELKSAITKFLTEEEIKSILERAE
                     AEPGDLICFMADKNEIVYNALGQLRLELARRLELLNKNEFKFLWVTEFPLLEFDEVEK
                     RWVAKHHPFTSPMDEDIQYLESDPERVRAKAYDIVLNGVELGGGSIRIHNQELQSKMF
                     KLLGFSQEQAWERFGFLLEAFKYGTPPHGGMAFGFDRMIMLMTGKDSIRDVIAFPKVQ
                     NASCPMTNAPGEVDEKQLKELRIKGC"
     gene            complement(2108..3364)
                     /gene="hisS"
                     /locus_tag="PK733_18340"
     CDS             complement(2108..3364)
                     /gene="hisS"
                     /locus_tag="PK733_18340"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOJ12528.1"
                     /translation="MLTKAPKGTRDILPEEVYKWHYLEKVISKICRNFGYKEIRIPVF
                     EHTELFQRGVGDTTDIVQKEMYTFQDKGGRSITLRPEGTAGVVRSYIENGMASLAQPV
                     KLYYNITAYRYENVQKGRYREFNQFGAEAFGAEGPSVDVEIISLVDLFFKKLGLKEIE
                     LNINSIGCPECRKEYNLRLKEYLQPLLVDLCPVCQERYDRNPLRILDCKEEKCKNLVK
                     DAPALLDNLCSGCREHFEGLKAGLENLEIKYNIDKNIVRGLDYYTRTVFEFVSLNVGT
                     QGTICGGGRYDGLVELCGGTPAPGIGFALGMERLLMEMDKQNVEIPMPDGVDIYFASL
                     GERASRYASKLVFELRGAGINAESDLMGRSLKSQMKYADKLGAAFTVVIGDDEIDSNK
                     AILKNMKTGEQKDVSLDSILTRLKNK"
BASE COUNT         1216 a          973 c          585 g         1415 t
ORIGIN      
        1 aagtatagca aaagctatat gcaacaccca atcaagaatt tcttttaaaa taggtttctc
       61 cgccaaagaa atccctccat ttcatttaaa tcaataaaag gggacagtgg ggtgcattta
      121 ccaaaagtga agaaataatt accagaagct gtcgttacgc tccctgccaa tattttattc
      181 acttaaatta aatacacctg tgacaatgct cattatagaa gaatgtcccc tcaccttagc
      241 accttagcac cccttaatcc gcagctcctt aagctgcttt tcatccactt cacctggagc
      301 attagtcata ggacatgaag cattctgtac tttcgggaat gctattacat cccttatgga
      361 atcttttccg gtcataagca ttatcattct gtcaaagcca aaggccatac ctccatgggg
      421 aggagtacca tatttgaaag cttcaagcag gaaaccaaat ctttcccatg cctgttcctg
      481 gctgaagcca aggagtttaa acatttttga ctgaagctcc tggttgtgta ttcttatgct
      541 cccacctccc agttcaactc cgttcaatac gatatcatat gcttttgccc ttactctttc
      601 agggtctgac tccaaatatt gtatatcttc atccattgga gaagtaaatg ggtgatgctt
      661 tgctacccag cgcttttcca cttcatcaaa ctcaaggaga gggaactcgg taacccataa
      721 aaacttaaat tcattcttat ttaataattc aagtcttctg gccagttcaa gccttagttg
      781 accaagagca ttatatacta tttcgttttt atcggccata aagcaaatta agtcacctgg
      841 ttctgcttca gccctttcaa gtatagactt tatctcttct tccgttaaaa actttgtaat
      901 ggcagacttc aattcatttt cctcaactac aatccaggcc ataccctttg ctttatatgt
      961 ttttacaaat tcaacaaggc tgtcaatctc acgtctgctg aatttttcgc cacagccctt
     1021 tgcattaatg agccttacac ttccgccatt tttaactgca tcggaaaata ctttaaatcc
     1081 gctgttttcg actatatcag aaatattaac cagttcaaag ccaaatctta tatcaggctt
     1141 atctgaacca aacctgtcca ttgcctcctg gtagggcatt ctgataaatg gttcggtaaa
     1201 ctctactcca agtgcttctt taaaagcttt tttaatgaag ccttcattta tttcaataac
     1261 atcctctaca ttaacaaaag acatttcaat atcaatctgt gtaaactcag gctgtctgtc
     1321 agctcttagg tcctcatctc tgaagcacct gactatttga aaatatctgt caaacccaga
     1381 aaccattaaa agttgtttaa aaagttgtgg tgactgaggc aaagcataga atttgcctgg
     1441 atgcaccctg ctggggacaa gataatctct ggcaccttca ggagtactct ttgtaagcat
     1501 cggggtctcc acttcaagaa atccttttga atcataataa tctctggcaa ttttagccac
     1561 tctatgcctt agaatgaaat ttttctgcat gtccggtctt ctaagatcaa gatatctata
     1621 tttaagcctt agcaattcat ttgcatcaga gtcttcttca atatatatag gaggagtttc
     1681 ggcctgacta agaatcctta attctcttac tgcaacttct atttctcctg tatctatctt
     1741 tggatttaca gtctccggat ccctcttgac aacttcacca actactgcaa taacatattc
     1801 acttctaaga ccttcagctt tcctgaaaat ttcactgttt tggtcaagac ggaatacaac
     1861 ctgcagaata ccggacctgt ctctcaaatc tataaagatt acgcccccca gatcccttct
     1921 cttatgcacc catcccataa ctgtaactgt ctttcctaca ttatccggtc gtaaatcatt
     1981 gcacatatat gtgcgtttca taccataaat tgactcttcc atatatactc cctccataat
     2041 ctgtcatatc attctttatt ttgacagtct catatttatt ttaacaaatt ttacaaattc
     2101 ctatctgtta cttattcttt agccgtgtca gtattgagtc caggctcaca tccttctgct
     2161 cacctgtttt catatttttc aaaatggctt tattactatc aatttcatca tcacctatta
     2221 caactgtaaa tgcagcacct aatttatccg catatttcat ctgtgattta agacttcttc
     2281 ccatcaagtc gctctcggca tttattcctg caccccttaa ctcaaatacc agctttgatg
     2341 catacctgga agctctttca cccaaggatg caaaataaat gtctactccg tcgggcatag
     2401 gaatttccac attctgctta tccatttcca taagaagccg ctccattcca agggcaaatc
     2461 ctattcctgg tgcaggtgtt cctccacata gttctactaa tccatcatac cttcctcccc
     2521 cgcaaatagt cccctgagtt ccaacattta atgatacaaa ttcaaataca gttcttgtat
     2581 aataatccag gcctcttaca atattcttat ctatattata tttaatttca aggttctcaa
     2641 ggccggcttt caatccttcg aaatgctctc tgcaaccact gcataagttg tccagtaaag
     2701 caggtgcatc tttaacaagg tttttgcact tttcttcttt acaatccaat attctcaatg
     2761 gatttctgtc ataccgttct tgacaaacgg gacaaagatc aacgagtaat ggctgtaaat
     2821 actcttttaa tcttaaattg tattctttcc tgcattcagg acaaccgatg ctgtttatat
     2881 tcaactcaat ttcttttaat ccaagcttct tgaaaaacaa atcaaccagg ctgataattt
     2941 ccacatcaac agaaggtccc tctgcaccaa aagcttctgc cccaaattga ttaaactccc
     3001 ggtatcttcc tttttgaaca ttttcgtacc tgtaggccgt aatattataa taaagcttta
     3061 caggctgtgc aagagatgcc ataccatttt caatatagct tctcacaacg cctgcagtac
     3121 cttcaggcct taaggttatg cttcttccac ccttatcctg gaaagtatac atttcctttt
     3181 gcacaatatc ggtagtatct ccaacaccac gttgaaaaag ctccgtatgt tcaaaaacag
     3241 gaatacgtat ttccttgtac ccaaaattcc tgcatatttt tgaaataact ttttccaagt
     3301 aatgccattt atacacttcc tccggaagta tgtccctggt tcctttagga gcttttgtaa
     3361 gcattctcat cttcctctca taaaaaaatc taatattcat tacctctaaa atacttcatt
     3421 atataaaaac ctatatataa aaacctcccg cctgacttaa acatttattg cattcatcag
     3481 ggacgagagg ttgtttcccg cggtaccacc ctattaggta taaaaaaacc cacttcaggc
     3541 ccctttaacg tcgggcatac ggattaacct actaaattat aatcagtata tacaatattt
     3601 cgcaaataga taccaactta tgcttagtca tggtaattga taccgtattg tatgttcata
     3661 ataattaata ccaaatatat tgattataat ctttcagcaa accaactccg gggtgttctt
     3721 tcaccaacct ttttcctggg aacactctca gtcctggatg tcccctccct gccggcagta
     3781 ttgggtactt ttccccatca tagtttttta tatatctcta attccagata tcttctaaat
     3841 atctattgaa ttaattatcc attaaattaa ttatctacaa aatatatcta ttaaatatat
     3901 tctatttctt ttctatatgt atatgcaaca tttttttatt attttctata aatttttctt
     3961 taattttaaa ttttgaatac ttttttagtt atattttaaa tatctatcct tgtaatgtca
     4021 atagggtgaa aatttatagg gggtgcattt tcttcctatg aaacaaaata tggcagcaaa
     4081 cgattttccg agtataaaat ttgaacaaag tttacggatt gttatgttta aggcaaggga
     4141 aaattcgtaa ttcacgacac ggatgtcgtg agccgtcacc tgaatcatg
//
LOCUS       DAOSDD010000323         4090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7716_length_4090_cov_5.917224, whole genome shotgun sequence.
ACCESSION   DAOSDD010000323 DAOSDD010000000
VERSION     DAOSDD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7716_length_4090_cov_5.917224"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK733_18345"
     CDS             <1..240
                     /locus_tag="PK733_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12529.1"
                     /translation="NLIVSKDKSSAFMFDYNLRNRKIKAIKALIYSAIKAEDETVTLK
                     EVGKMLTLNDMERLGTAINEALSVAMPEIEENMGE"
     gene            864..1226
                     /locus_tag="PK733_18350"
     CDS             864..1226
                     /locus_tag="PK733_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ12530.1"
                     /translation="MKYYKLAETEEKFAELIWQNEPIGSGDLVKLSEKEMNWKKSTTY
                     TVLKKLCGKGIFQNENAVVSSLISKDEYYANQSIRFVEDTFGGSLPKFLTSFISGKKL
                     SNHQAEELKRLIDEHKGV"
     gene            1229..3532
                     /locus_tag="PK733_18355"
     CDS             1229..3532
                     /locus_tag="PK733_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ12531.1"
                     /translation="MSELFLTVLNMSLTASYVILFVILIRLLLKKAPKFIAYALWGVV
                     AFRLIIPFSFESMFSLMPRNTNAVPIPQDIIYQQSLQVNSGIGVVDSLVNNSLPAPTI
                     GASVNPLQIYVEIGAYIWALGIIALLVYSLVSILVLKRQLKSAQLIEKNIFEAKNLKT
                     PFVLGLIKPKIYLPVGLNATERSYILLHEQTHIHRKDHIIKVLAFLTLSVHWFNPLVW
                     IAFILMSKDMELSCDERVLKQMNNEDIKKPYANSLLSLATGKHILNGSPLAFGEGNVK
                     GRIKNVLNYKKPAFWVIVLSIVILTVAGIGLMANPKAATIEPTAPELSFEQTVGVDMV
                     QLDYASNDIVIFHDYFGLFVYDLNSHQIVRSLDLKPLNCHQTQGDNYCDVSVSMNGNT
                     VQLHPMSSENMFVYTISDNTLKEKTHMPMEKPFGSQFVSIEEVINSTKFGNHSHHAVL
                     FDTGEHGYLYAEDGTIGTLSYVRGDKVFRLFDIKASLSDRRPMIMVNGVLYLDTGKQV
                     PVEINDSAIIGETSSSVDQSEKPTEEGQTNFGSIGSKYAYYEENIVVLINNKWILFER
                     DNNIYQLNFSEIDYTKIEKLQLNNINIIEEKIIDKDLSAFIFTNNENGEYKGAINLHG
                     KSYYIGQVSMESTPEDLMGIEELQVFGKRAVKFYGILGANYAQAYYWLVEEKTEDSIF
                     QVDGNTIEIDLDSDDKNEIVATIGTIPETRIYKLEEGKIYVSDINKSIGAKSVYLKDE
                     GKKLFEVYFEPNKPEQYVFNKDSFIKK"
     gene            3605..3883
                     /locus_tag="PK733_18360"
     CDS             3605..3883
                     /locus_tag="PK733_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12532.1"
                     /translation="MADFLMQYGEVFSIIMFFIPPILSMLILYFTKRNWIWMSIPITI
                     VVDLLVWGKGIFELSHGRVALIFLIPQVTVVTIISFVILYLEKRRKRA"
BASE COUNT         1425 a          592 c          823 g         1250 t
ORIGIN      
        1 aacctaatag tttcaaaaga caagtcttca gcatttatgt ttgattataa tttacggaat
       61 agaaagatta aagcaattaa agcactaata tactcagcaa ttaaagctga agatgaaact
      121 gttacactta aagaagtagg aaaaatgctt actttaaatg atatggagag gttaggcact
      181 gcaattaatg aggcattaag tgttgcaatg ccagagatag aagaaaatat gggggaatag
      241 aaagccacac tgattctgaa ggatgggatt gggagtggcg aaaatgggga gctccccatt
      301 tgagacagat gaaggcattg cacatttatg aaggtgggta cactttttca gaagtattaa
      361 atttgacatg tttccttgaa cgagccacgt tgagtgcgtg attctgatgc agcatagtgg
      421 tgtagaggat aaaaaatagg gtatgaccac tacatgtagt ggttttaagg taaaaaacaa
      481 gcaaaaaagg gggttaaaaa tgcacgattt taaccccctt ttttagcgaa aaatactgat
      541 gcagaggcta attttttaga tgcaaccctt aaaatgatgc aaaaccactt taaggtaagt
      601 taagattata aaataaaata acaatagaaa aacagacatt atttgaaacg atattggtgt
      661 aagttttaaa tagggaattg aagttgaagt ataaaatatt acttggaggc agaccgtcaa
      721 tctggagata aactctcctt attgacggtt tttataatct taaatcttgt tacatttttt
      781 caaacaaatt gtcattgaca atttggttta tacagtatag acttaatata aagtcgatgt
      841 tgaatagacc aaggaggttt atcatgaaat attataaact cgcagaaact gaagaaaagt
      901 ttgcagaatt aatctggcag aacgaaccga ttggttcagg cgaccttgtt aagctaagtg
      961 aaaaagaaat gaattggaaa aagtccacaa catatacagt acttaaaaag ttatgtggta
     1021 agggtatttt tcaaaatgaa aatgcagtgg tttcatcttt aatttcaaag gatgaatatt
     1081 acgcaaatca aagtatacgt tttgtggagg atacctttgg agggtcactg ccgaaatttc
     1141 taacatcttt tatcagtggt aaaaaattaa gtaatcatca agctgaagag ttaaaaagat
     1201 tgattgatga gcacaagggg gtgtagcaat gagtgaacta tttcttactg ttttaaatat
     1261 gagccttacg gcaagttacg taatcctttt tgtaatacta attaggctgt tacttaaaaa
     1321 ggctccaaaa tttatcgcct atgccttatg gggtgtggtt gcttttcgcc ttataattcc
     1381 attctccttt gaaagcatgt ttagtcttat gccacggaat acgaatgctg taccaatccc
     1441 ccaagatatt atctatcagc aaagtctgca ggttaacagt gggataggag tagttgactc
     1501 attagtaaac aattcacttc ccgcaccgac tattggagca agtgtgaatc cacttcagat
     1561 ttacgtggaa ataggagcgt acatctgggc cttaggcata atagcattgc ttgtgtacag
     1621 ccttgtatct attttagtct taaaaaggca gcttaaaagt gcacaattaa tagagaaaaa
     1681 tatcttcgaa gctaagaatt tgaaaacgcc atttgtgctt ggattaataa aacccaagat
     1741 atatttaccg gttggactta atgctaccga aagaagctat attttgctac atgaacagac
     1801 ccatatccac cgaaaagacc acatcattaa ggtgttagct ttcctaacat tgtctgtgca
     1861 ctggttcaat ccccttgtat ggattgcgtt tatattaatg agtaaggata tggaactatc
     1921 ctgtgacgaa agagtactga aacagatgaa taatgaagat attaaaaagc cttatgctaa
     1981 ttcattattg tcgcttgcta ccggaaagca tattttaaat ggtagccctc ttgcttttgg
     2041 cgagggaaat gtaaaaggga ggattaaaaa cgtgttgaac tataagaagc ctgcattttg
     2101 ggtaattgtt ttgtcgattg ttattttgac tgtagctgga attggactga tggcaaatcc
     2161 gaaagctgca actattgaac ctactgctcc agagttgtct ttcgagcaaa ctgtgggagt
     2221 tgatatggtt cagcttgact atgcttctaa tgatatagta atttttcacg actatttcgg
     2281 attgtttgta tatgacctta actctcatca aattgtccga agtttggatc ttaagcctct
     2341 aaactgccac caaacacaag gagataatta ctgtgatgtt tcggtcagta tgaatggtaa
     2401 cacagtgcaa ttacacccta tgagcagtga aaatatgttt gtatatacca tttcagataa
     2461 taccttaaag gaaaaaactc atatgccaat ggagaaacca tttggaagtc agtttgtttc
     2521 catagaagag gtgattaatt caacaaagtt tggtaatcac agccaccatg cagtcctctt
     2581 tgatacaggc gaacatggat atctctatgc agaagatgga acaatcggca cgctgtcata
     2641 tgtaagagga gacaaggtat tcagattgtt tgatataaaa gcaagcttat ccgatagaag
     2701 gccaatgatt atggtcaatg gtgtgttata tctagatacc gggaaacaag tgccggttga
     2761 aattaatgat agtgcaatta ttggagaaac cagctcttca gttgaccagt ctgaaaaacc
     2821 aactgaagaa ggccaaacaa actttggttc aataggttca aaatatgctt attatgaaga
     2881 aaatattgtt gttttaatta ataacaaatg gattttattt gaaagagata ataacatata
     2941 tcaattaaac ttttctgaaa tagattatac gaaaattgaa aaattgcagc ttaataatat
     3001 taatattatt gaagaaaaaa taatcgataa agaccttagt gcatttatct ttacgaataa
     3061 tgaaaatggt gaatataaag gcgcaataaa cttgcatggg aaatcttatt atataggtca
     3121 agtgtcaatg gagagcacac cagaagattt aatgggaatt gaagaacttc aggtatttgg
     3181 taagcgagct gtaaagtttt atggaatact tggagccaat tatgctcagg catattactg
     3241 gcttgttgaa gaaaaaaccg aagattcaat ttttcaggta gatggaaaca caattgaaat
     3301 tgatttggat agtgatgata aaaatgaaat tgtagcaact ataggcacaa ttcctgaaac
     3361 aagaatatac aagctggaag aaggaaaaat atatgtttct gatattaata aatcaatcgg
     3421 cgcaaaatcg gtatatttga aggatgaagg taaaaagtta tttgaagtgt actttgagcc
     3481 aaataagcca gaacaatatg tgttcaataa agattctttt ataaaaaagt agaaccacta
     3541 ctatacaaga ccgaaaagat ccgggcttac agcaagaatt atttatattt gatgaggtga
     3601 tgttatggca gactttttaa tgcaatatgg tgaggtgttt tccatcatta tgttttttat
     3661 cccaccaatt ttgtcgatgc taatattata ttttactaaa agaaattgga tatggatgtc
     3721 tataccaatt acgatagttg ttgatttatt agtttgggga aagggaattt ttgaattatc
     3781 acatggtaga gtagcgctta tctttttgat accccaggtt acagttgtga caatcatttc
     3841 tttcgtaatt ctctaccttg agaagcgtag aaaaagagca taatcctggt tcctatatga
     3901 cattttttat attctactca acagtagatt tatgttcaaa tagttacaaa ttaacaattg
     3961 aattatcata ctaataaata ggagtttgat ggaggaagac gatgaatgtg cgcctgttcg
     4021 gtgattaata tataatgggt gcgaatccca tctggtaaag cctagccagc aaccagtact
     4081 tagccttgga
//
LOCUS       DAOSDD010000324         4067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7774_length_4067_cov_3.493769, whole genome shotgun sequence.
ACCESSION   DAOSDD010000324 DAOSDD010000000
VERSION     DAOSDD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7774_length_4067_cov_3.493769"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PK733_18365"
     CDS             complement(<1..320)
                     /locus_tag="PK733_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ12533.1"
                     /translation="MIIGVFALILAIITLYLVSAGKYDDFIKPLDKREHILKRFLPIG
                     FYIMDGIGYRYNSQYDKLLLMKIIEIHGAKNADYYLRIHWASKIVIFILGLFFAVLLG
                     AGSGV"
     gene            complement(559..1353)
                     /locus_tag="PK733_18370"
     CDS             complement(559..1353)
                     /locus_tag="PK733_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadB"
                     /protein_id="HOJ12534.1"
                     /translation="MGKNIYKAEKANDQRVGMIDYNVYEMSVKEKVFNAAKVMPVIYL
                     LSYLLYKNHLLSFMISFSALLYPRFKVKKIINKRKEDLNVQFKDMLYSLQSSLWAGKP
                     IELAFREVVKDLHILYPDPETYIILESSNIAGKIEMNETVENAVADFGERSGIDDIQD
                     FAEVLQICKRTGGNLVEVVKRTTSIINDRIEMKQDINTMLAKRNLERRVLNIIPVAVL
                     ILLSVTADDYINPVYCTVTGRIAMTFAALMFVIAYLISKKIMDIDI"
     gene            complement(1416..2717)
                     /locus_tag="PK733_18375"
     CDS             complement(1416..2717)
                     /locus_tag="PK733_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOJ12535.1"
                     /translation="MMNNRETATNQIIENNYNDAKGNLTGVIKAIVSNRIDIRKDITD
                     TEIRELITSVVFEKSQQVYLSIKEKQEIVESVFNSMRRMGILQPLIDDHLVTEIMING
                     PDNIFIEKGGSVHKLDIKLENEEKLEDIIQSVVSKANRVVNEASPIVDARLQDGSRVN
                     VVLKPIALNGPVMTIRKFPSEPVTMNKLIELGSITPKGAEFLKYLVKAKYNIFVCGGA
                     GSGKTTFLNALSGFISEDERIITIEDSAELQITGISNLVRLETRNENMEGKGRVSVKD
                     LIKTSLRMRPERIIVGEVRGDEALDMLQAMNTGHDGSLSTGHGNSPRDMLNRLETMVL
                     GAAPLPLQAIRQQIASAIEIIVHLSRMRDKSRKTLEITEVAGFKEGEIVLNPLFEFED
                     EGDTEGQTVKGSLKRTGNEMIRKMKFKQAGVWKQGDDSLEA"
     gene            complement(2714..3952)
                     /locus_tag="PK733_18380"
     CDS             complement(2714..3952)
                     /locus_tag="PK733_18380"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12536.1"
                     /translation="MLKLSLIIADEDKAYIESLVGFLMDKHPHKFQINSFTNRDYLLE
                     FLSKSREKADILLITPDFHDRLKYNTLKSNVLEFNNIESTGLKSDISEYRKPVLIFLS
                     NTKSAFTSGTVDTIYKYQHGDRLVSDIIHIYEKQYKNIEFALKGPDKTKLISVYSPAG
                     GTGKTTIAVNACINCAREEMRVFYVNLETIGSTLCFFEHDYENRPYDNFSSVIFAIKE
                     KDKNLHAKIEGIKFTDTMYDIHYIPPPDSALEMEEILPEEVRYFLNQVRSTGYYNIIF
                     ADLSSALSSKNIAVMEESDEIVLIYTPDCISKSKIISFYKEIQLYQKRKGLNLLNKIT
                     LILNKYDEHIPQEIKNEIDEVCSIMGITHYIKIPMNNQVLYNQGTYLKGIVEESNSLN
                     KELRKLTGKYTVRGDSDLFQ"
BASE COUNT         1195 a          844 c          518 g         1510 t
ORIGIN      
        1 acaccagatc cagctcctaa taaaacagca aaaaataatc ctaatataaa aattacaatt
       61 ttactggccc aatgaattct aagataatag tcggcatttt ttgctccatg aatttctata
      121 atcttcataa gcaacaattt atcatattgg gagttatacc tataacctat tccatccata
      181 atgtaaaacc ctataggtaa aaaccttttt aatatatgct ctcttttatc cagaggctta
      241 ataaaatcat catattttcc tgctgaaacc aggtataaag tgataattgc taatattaaa
      301 gcaaatactc ctataatcac aatttataac ccccttgtat atcttcattt accgtccttt
      361 gccaaccttt aaaactcttt atagtttctt attgctttat attgttctat gttatgtttt
      421 tatgtctctt acaataactt acaattcctt cataatgctt tacataatgc tttacataat
      481 gctttaaacc ctttgaagtc cttcaaaatt cttcacctta tattattgct caatatcacg
      541 tgacaatcgt tttttacctt atatatctat atccattatc ttttttgata taaggtaagc
      601 tataacaaac attaacgctg caaatgtcat tgcaattctg ccggttaccg tgcaatatac
      661 aggatttata tagtcatctg cagttaccga aagaagtatt aaaactgcaa caggtatgat
      721 atttaatact cttctttcaa gatttctttt agccagcatt gtattaatat cctgcttcat
      781 ttcaattctg tcattaatta tgcttgttgt ccttttaact acttcaacaa gattacctcc
      841 cgtcctcttg cagatctgca atacctctgc aaagtcttga atatcatcaa taccggatct
      901 ctctccaaaa tctgcaacag cattttccac tgtttcattc atttcaattt ttccagctat
      961 attagaactc tccagtataa tataggtttc gggatcaggg taaagaatat gaaggtcttt
     1021 tactacttcc ctaaaagcaa gctcaatggg tttccctgcc cataaagaag attgtagaga
     1081 ataaagcata tctttaaatt gcacgtttaa atcctctttc cttttattga taattttttt
     1141 gactttgaaa cgaggataaa gcaatgcact aaaacttatc ataaaactta gtaaatggtt
     1201 tttatatagc agataactca ataaataaat gacaggcata acctttgccg cattgaaaac
     1261 cttttccttt acagacattt catatacatt ataatctatc attcctaccc tctgatcatt
     1321 agctttttct gctttatata tatttttacc cattacttta cctacactcc tcttttgcca
     1381 acttgcctgt tttattacat tactttatat tgtccctatg cttccaatga atcgtccccc
     1441 tgcttccaca cacctgcctg cttaaatttc atcttcctga tcatttcatt acccgtcctt
     1501 ttaagagagc ctttcactgt ctggccttct gtatcccctt cgtcctcaaa ctcaaacaaa
     1561 gggttcaata ctatttctcc ttccttgaaa cctgcaactt cagttatttc aagggttttt
     1621 ctggacttat ccctcatcct ggaaagatgt actattattt caatggcaga agctatctgc
     1681 tggcgtattg cctgaagagg caaaggtgct gcgcccagta ccatagtctc cagccgattt
     1741 aacatatccc tgggggaatt tccatgtcct gtggataagg atccatcatg ccccgtgttc
     1801 atggcctgca acatatcaag agcttcatct cctctcactt cccctactat tatcctttca
     1861 ggcctcattc tgagggaagt tttaataaga tccttaacac ttactcttcc cttaccttcc
     1921 atgttttcat ttcttgtttc caatctaacc aagttactaa tacctgttat ctgaagctca
     1981 gccgagtctt ctatggtaat gattctttca tcttcagaaa taaaaccaga taaagcattt
     2041 aaaaatgtag tttttcccga tcctgcaccc ccacaaacaa aaatattata ttttgccttt
     2101 acaagatatt ttagaaattc agctcctttc ggagtaatag atcccaattc aataagttta
     2161 ttcattgtta caggttcact tgggaattta cgaattgtca taaccggtcc atttagtgct
     2221 atgggcttta acactacatt aaccctggag ccgtcctgca gcctggcatc tacaattggg
     2281 gaggcttcat ttaccactct gtttgctttt gagactaccg actgaattat atcctcgagt
     2341 ttttcttcat tttcaagctt aatgtctagc ttatgtacac tacccccttt ttctataaaa
     2401 atattgtccg gtccgtttat cattatttct gtcactaagt gatcatcaat aaggggttgc
     2461 aaaattccca tccttctcat agagttaaaa acagattcta caatttcctg cttctccttt
     2521 atactcagat aaacctgctg tgatttttca aaaactacac ttgtaataag ctctctaatt
     2581 tctgtatctg taatatcttt tcttatatct attctgttac tgacgatggc ctttattacc
     2641 cctgtaagat ttcctttagc atcattataa ttattctcaa ttatttgatt ggtagcagtt
     2701 tctctattat tcatcattga aataaatcac tatcccccct tacagtatat tttcccgtta
     2761 atttccgtaa ttctttgtta agactgtttg attcctctac tatacctttt aagtatgttc
     2821 cctggttata caaaacctga ttattcatgg gaattttaat atagtgggtt attcccataa
     2881 tagagcatac ttcgtcaatt tcgtttttaa tttcttgtgg aatgtgttca tcatatttat
     2941 ttaatatcaa tgttatttta ttcagcagat ttaaaccttt tcttttctgg taaagctgaa
     3001 tttctttgta aaaacttata attttcgact ttgatatgca atctggagtg tagattaata
     3061 caatttcatc actttcctcc atcacagcta tattttttga actcaatgca ctggataaat
     3121 cagcaaagat aatattgtaa taccctgtag accttacctg gttcaagaag tacctcactt
     3181 cctcaggtaa tatctcttcc atttccaaag cactgtcagg aggcggtata taatgaatat
     3241 catacattgt atcggtaaac ttaattcctt ctatttttgc gtgaagattt ttatcttttt
     3301 ccttaatagc aaaaatgaca ctggagaaat tatcgtaagg cctattttca taatcatgct
     3361 caaaaaagca aagggttgag ccaatagttt ccaggttaac ataaaatact ctcatttcct
     3421 cccgagcaca gttaatacag gcattaacag caattgtagt ttttcctgtg ccaccggcag
     3481 gtgaataaac tgatatgagt tttgttttat ccggcccttt caaagcaaat tctatatttt
     3541 tatactgttt ttcatatata tgtataatat cacttaccaa cctgtcacca tgttgatatt
     3601 tataaatagt atcaacggta ccggatgtaa aagccgattt agtattggac aaaaaaatta
     3661 aaacaggttt cctgtactct gatatatcag attttaatcc ggtagactct atattattaa
     3721 attctaaaac gttagatttc aaagtattat atttcaatct gtcatggaag tctggagtaa
     3781 taagaagtat atcggctttt tctctgcttt ttgaaagaaa ttctaaaaga taatccctat
     3841 tcgtaaagga atttatctgg aatttatgag gatgcttgtc cattaaaaac cctaccaggc
     3901 tttcaatata tgccttatcc tcatctgcaa ttatcaagct taatttcaac atatttcctc
     3961 accatttccc tttttatata tagtttattt tttgttccca tatatgtatt atatttccat
     4021 gcaaacacat tatattacat aaaccttttt ttgtcaatta taaatat
//
LOCUS       DAOSDD010000325         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7785_length_4063_cov_4.879242, whole genome shotgun sequence.
ACCESSION   DAOSDD010000325 DAOSDD010000000
VERSION     DAOSDD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_4063_cov_4.879242"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..282
                     /locus_tag="PK733_18385"
     CDS             70..282
                     /locus_tag="PK733_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ12537.1"
                     /translation="MKIKIKELRKQRKIAQEELALAVGTTRQTITSIEVGKYTASLVL
                     AYKIARYFDLTIEEVFDFSEFNEVNK"
     gene            293..742
                     /locus_tag="PK733_18390"
     CDS             293..742
                     /locus_tag="PK733_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12538.1"
                     /translation="MEKYRKTIRKRMILCGFLAAVAVGLGILHQSEFFDLISLYTPNE
                     ALAGFQAGLLSGFGVLSLLLLYKYGKAVKDDTKLKILYNKEYDERQNLIRQKAGMPML
                     MITSGIMIFAGIIAGYLNEIVFYTLILAAMIQLTLGAMVKIYYMRKM"
     gene            783..1100
                     /locus_tag="PK733_18395"
     CDS             783..1100
                     /locus_tag="PK733_18395"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOJ12539.1"
                     /translation="MNCSVWVAWLMNLLCAVISLMFLMGKGSLLIAGYNTSTREIKAT
                     YNVKRLCRVVGGGFSVITIILVIYTIYEFDLPSYLQWMMPWGFFAVIAFIAILANSVC
                     KKK"
     gene            1278..3398
                     /locus_tag="PK733_18400"
     CDS             1278..3398
                     /locus_tag="PK733_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOJ12540.1"
                     /translation="MIFIKKLFGRISIPVSMLLSMQTMRILCTLFSIIMTLQALIGLS
                     SIGKVFAAVNDNKEITILFTHDMHDNLLPFKTEQGGKIVQSGGYARLQSAINSERNKD
                     PELLLLDAGDFSMGTLFQTLFSSDAPELRVMGQMGYDVATFGNHEFDYRGKGLADSLD
                     SAVNSGDKLPQIAASNISFPTDKNGNIAESLTNLKRVMDKYGVKDYVVLERKGIKIGV
                     FGLMGKEAASYAPMSEVVFMDPIKKAKSIIDILKNDEKVDLVICLSHSGTWTDKSKSE
                     DEILAKKVPEIDVIISGHTHTKLTEPIIAGKTIIGSCGENSENLGIIRISQDSNNANN
                     TNNTGNTGNTGNGDNAKNKSNNSWNLINYNLKPIDGGLSEDFYISQAIGLFKDKVQEK
                     YLDKLGMEFDEVLANTGFNFVSASKIGDEHKEDTLGNLISDAYIYAVQKAEGSNYEPV
                     TAAIVPVGTIRGSFVEGPITVSDAFNVSSLGISKDGMSGYPLISVYLTGKELKTACEV
                     DASITPIMGEAQLYISGLSYSFNPKRLMFNKVTMARLQKPDGTFEEIDDKKLYRVVAG
                     LYSAQMLSVVGRKSFGLLPIIPKTKDGSTITDFEAQIITNSSNWRNNEIKEWMAIAEY
                     LKSFEIVGDIPQVPMYYSQTHGRKVVETKADILSILGNPNGIALAVYAVVAIVVILAF
                     FIIYVLIRLRKSSRRKVYNWSMRI"
     gene            complement(3800..>4063)
                     /locus_tag="PK733_18405"
     CDS             complement(3800..>4063)
                     /locus_tag="PK733_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOJ12541.1"
                     /translation="GSRLLRRVLFTTALANIRTKRNKQACNPVLLEFYKQKCQSKPKK
                     VALGAVMRKIVCIIFAVLRDRKPYELRSPQEHAQMLAAKHSAA"
BASE COUNT         1391 a          610 c          802 g         1260 t
ORIGIN      
        1 catgcaaagt aaattatatt aaaaatgcaa agttaacatt gcaaatatga ttcgaatgag
       61 gtaccatatg tgaaaattaa aattaaagag ctgcgaaagc agagaaaaat agcacaagaa
      121 gaattggcat tagctgtggg aacaactaga cagacaatta cttctataga ggttggtaag
      181 tataccgcat cattagtgtt agcatataaa attgccagat attttgattt gacaattgag
      241 gaagtatttg atttttcgga atttaatgag gtaaataagt aggaggttac tgatggaaaa
      301 gtatagaaaa acaataagaa agagaatgat tctatgtgga tttttagcag ctgtagctgt
      361 tggcttaggc attttacacc aaagtgaatt ttttgatttg atatctttat atacgccaaa
      421 tgaagcatta gccggttttc aggcaggact tctttctggt tttggagtac tgtcactgct
      481 tttattgtat aaatatggaa aagcagttaa agatgacaca aagttaaaga tactgtataa
      541 caaggagtat gacgaaaggc agaatttaat ccgacagaaa gcaggaatgc caatgttaat
      601 gattacttca ggaattatga tttttgcagg tattattgca ggatatttaa atgaaattgt
      661 tttctatacc ttaatattgg cagcaatgat tcaattgaca ttgggagcaa tggttaagat
      721 ctattacatg agaaaaatgt aaaagaaatc tgaaaggaag taaactgatt gggggaaaag
      781 atatgaattg ttctgtatgg gttgcctggc taatgaattt attatgtgca gttatatcct
      841 tgatgtttct tatgggaaag ggaagtttac taatagctgg gtataatact tcaacacggg
      901 aaataaaagc aacatataat gtcaaacgat tgtgccgtgt tgtaggtggt ggtttttccg
      961 taattacaat tattcttgta atttatacca tttatgaatt tgatttacct tcttatctgc
     1021 aatggatgat gccttgggga ttttttgccg tcattgcttt cattgcaatt ttggcaaatt
     1081 ccgtttgcaa gaaaaaatga ctttgttctt tgaagaaccg tcacctttta gacctagtgg
     1141 tattgttgca aattattttt aaaaaatgta atgcaattaa gattagaaat aatatataat
     1201 aatattagca aacaattaac cggagatgaa aattgcatat atcgagaaat acgaacgata
     1261 tctttaatag atagggagtg atttttatta aaaaattatt cggacggata tccatacctg
     1321 tatctatgtt attatctatg cagactatgc gtatattatg tacactattt tctattatca
     1381 tgactttgca ggctttaatc ggcctgtcca gtataggtaa ggtatttgcg gcagtaaatg
     1441 ataataagga aattaccata cttttcactc acgatatgca cgataattta ttgcctttta
     1501 agaccgagca gggtggtaaa attgttcaat ccggcggata tgcaagatta caaagtgcta
     1561 tcaattcaga aagaaacaaa gatcctgaac tgcttcttct ggatgcaggg gatttttcca
     1621 tgggaactct ttttcagacc ttattctcaa gtgatgcccc ggaactcaga gtaatgggac
     1681 aaatggggta tgatgttgca acctttggca atcatgaatt tgattataga ggcaaggggc
     1741 tggcagattc cttggattct gccgtaaaca gcggcgataa attgccgcaa atagcggctt
     1801 caaatatttc atttccaact gataaaaacg gaaatattgc agaatctcta accaatttaa
     1861 aaagggtaat ggataaatat ggagtaaaag attacgtagt attagagcgt aagggtataa
     1921 aaatcggagt atttggctta atgggaaaag aggctgcatc ctatgctccc atgtctgaag
     1981 ttgtctttat ggatccaatt aaaaaggcaa aaagcataat tgatatatta aagaatgatg
     2041 aaaaagttga tcttgtaatt tgtctctctc attccggaac ctggacggat aaatctaaat
     2101 ctgaagatga aatccttgca aaaaaagtcc cggaaattga tgtaataata agtggacaca
     2161 cacatacgaa actaacagaa cctattattg caggaaaaac cataatcggc tcctgcggtg
     2221 agaacagtga aaatctggga attataagga tttcacagga ttctaataat gcaaataata
     2281 caaacaatac aggcaataca ggtaatactg gcaatggtga taatgcaaaa aataagagta
     2341 ataatagctg gaatttgata aactacaatc tgaagcctat cgatggcggc ttgtcagaag
     2401 atttctacat ttctcaggct attgggctat ttaaagacaa ggttcaagaa aaataccttg
     2461 ataagctagg catggaattt gatgaagtac ttgcaaacac aggatttaat tttgtttccg
     2521 catcaaaaat cggtgatgaa cataaagaag atactctggg aaatttaatc agtgatgctt
     2581 atatatatgc tgttcaaaaa gctgaaggtt caaattacga gcctgtaact gcggcaattg
     2641 tccctgttgg aactattaga ggttcctttg ttgaaggacc gataactgtt tcagatgcct
     2701 ttaatgttag ctcattggga atcagtaagg atggcatgtc aggatatccc ttgatttctg
     2761 tatatcttac aggaaaagaa ctgaaaacag cctgtgaagt ggatgcatca ataactccta
     2821 taatggggga ggcacagctt tatatatcag gactaagcta ttcattcaat ccgaagagat
     2881 tgatgtttaa taaagttacc atggcacggc ttcaaaaacc agacggtact tttgaggaaa
     2941 tagatgataa aaagctgtat cgcgttgtgg caggcttgta ttcagcgcag atgctttcag
     3001 ttgttggaag aaaatctttc ggactgctgc ctattatacc aaaaaccaaa gatggttcca
     3061 caataactga ctttgaagct caaataatta ctaatagctc taattggcgt aataacgaaa
     3121 ttaaagaatg gatggcaatt gcagaatact tgaaatcatt tgaaatagtt ggtgatatac
     3181 ctcaagtccc aatgtattac agtcagactc atggcagaaa agtcgttgaa accaaagcgg
     3241 atatcttatc gatattaggt aatccaaatg gtattgcttt ggcagtatat gctgtagttg
     3301 ctatagtagt cattcttgcc ttttttatca tatatgtatt aataaggcta agaaaaagca
     3361 gtagacgtaa agtatacaac tggagtatga gaatttaatt gtttgcataa tgcaccaaac
     3421 tgcttacata cttcttgcag agcagcttta ttgtctttcc tgaaattaga tattgcttta
     3481 aaaatcaggt cttgattttc taatcagcta tattatctct atatttctgg ttgtttctcc
     3541 ttcaaacttt cttgatggag taattctatc cgcatatcca tatttccatt cacattcttg
     3601 tggcagttta cataagatga tataaaaaga ccagctaatt aaagtcaaga ggttttaaca
     3661 aaaaaattta acaattttta aaaacttttg acagctaaaa aaggcataac aaataaacca
     3721 cccaagtgaa gttgacataa aatggctggt ctaaaataca atttgttctt tgaaaactaa
     3781 atatcaagta acagtactat taagctgcac tatgctttgc tgcaagcatt tgggcatgct
     3841 cttgtggaga acgtaattca taaggttttc tatccctgag cacagcaaaa ataatacaca
     3901 caatcttacg catgacagcc cctaaggcta ccttcttagg tttgctttgg cacttttgct
     3961 tataaaattc taataatacc gggttacaag cttgtttatt acgcttggtt cgaatattag
     4021 caagagcagt tgtaaaaagc actctgcgaa gtagtcgtga acc
//
LOCUS       DAOSDD010000326         4048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7823_length_4048_cov_6.394941, whole genome shotgun sequence.
ACCESSION   DAOSDD010000326 DAOSDD010000000
VERSION     DAOSDD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7823_length_4048_cov_6.394941"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..243
                     /locus_tag="PK733_18410"
     CDS             112..243
                     /locus_tag="PK733_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12542.1"
                     /translation="MKSILVNKKLSKRRSKAGYGKTPTGGSDDDSVETYITRNPEGS"
     gene            325..447
                     /locus_tag="PK733_18415"
     CDS             325..447
                     /locus_tag="PK733_18415"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12543.1"
                     /translation="MNGNAVGEHEGGSLPFEFDVTRFVKYNDINIFVFSIDAEP"
     gene            568..909
                     /locus_tag="PK733_18420"
     CDS             568..909
                     /locus_tag="PK733_18420"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12544.1"
                     /translation="MYCIPKTCIEDVSIKTDIKPGAAEISFVTKINKPLASDIRVYLN
                     NRLTGEYKLNGSDEITGSVLVEEPVLWDMDNPYLYNFKVVLHEEILMKINTGRCLILS
                     MGLVEAKMRQE"
     gene            1239..2354
                     /locus_tag="PK733_18425"
     CDS             1239..2354
                     /locus_tag="PK733_18425"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ12545.1"
                     /translation="MINKIDVGKRISSLRINLSYSQAEFAEKLSVSTQAVSKWETGRT
                     LPDIDVLLNISWICKTSINNILEGDDFIEYNTGIDRGLLRLNKFLICPECKKKLKLKS
                     LFFECENKHQYHIIDGVVDFRTREIPGEQWSLSYRNYDEYLHEHHWPGNPNYKRGLNP
                     ADVIWTEIEELRPRLILDMACGTGQGIKHQIKRINWPVTIVMVDLSHRILKWDKVFYS
                     TEWENPYVEMVYLACDGANLPILSDSIDVVFSYAGYESMQTKMMDGFREAFRVIKDGG
                     HTIYTKSVVEDHESENSKKWTNLLFSNLDHDEQFGMKDMMLDVNQWLQTCKDIGFIEN
                     NSIKIYDELPVPDTDKFPFENEIAQWMAEYVFNSRKP"
     gene            2633..2845
                     /locus_tag="PK733_18430"
     CDS             2633..2845
                     /locus_tag="PK733_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12546.1"
                     /translation="MIGQLHVEIYSSVLPYCRIEDVRFQEGETCDAMWASTDKIREMM
                     ASGEFLSEWFYPYFEEMVEKWGVIVC"
     gene            2839..3066
                     /locus_tag="PK733_18435"
     CDS             2839..3066
                     /locus_tag="PK733_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12547.1"
                     /translation="MLIRWAAEKDLPAWYALATEVSQIFQHPGNMGTDPEFASYAQSE
                     VSKYEVLTAVDYMSGKNMGFIEQMRKELRHA"
     gene            3059..>4048
                     /locus_tag="PK733_18440"
     CDS             3059..>4048
                     /locus_tag="PK733_18440"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOJ12548.1"
                     /translation="MPKLPNTLVILPYDPNWKTEFERIRDYLMEHIGDLVIEVKHIGS
                     TSVPGLCAKPIIDLVAVMESYEVFPQIVSRLEKVGFQHEGDLGIKEREVFKRIIPDDF
                     MDYHFYVYPKDSEENRRQTSFRNALLKNPQIAEEYGKLKTRLISEVNGDRVLYTNSKT
                     DFILDVINRVNDNGELKNISQNIYDNQTFFDGYKKLRENPDNANLLEEKPALFSLAPD
                     LTSLSVLDLGCGYGENCAEFLRLGAAKVVGVDISSKMLEVAKHETSGVEYIYADMSDL
                     SAIEGIFDVVFSSLAIHYIEDFSKLCYQVFDLLNSYGYFIFSQEHPFTTAPLKG"
BASE COUNT         1328 a          635 c          901 g         1184 t
ORIGIN      
        1 ttattgatta aaatttcggc tttttccagt aatgctttac tcatatcata accctaaaac
       61 acacgttcag tattaactta ccatataaca ctataaaaag caagtattaa catgaaaagt
      121 atcttagtta acaagaagct atcaaagaga cgttccaaag ctggatatgg aaagaccccg
      181 accggaggga gtgatgatga ttcagtagaa acatatataa ccaggaatcc ggaaggaagc
      241 taatagacag cagaggagag ctgttaaata tagacttcta cggagttctg gaggacatca
      301 caaacttatt gggcaaaggt atgggtgaac ggaaatgcag ttggagagca tgaaggcgga
      361 agtctgccat ttgaatttga tgtgacaagg tttgttaaat acaatgatat caatatattc
      421 gtgttttcaa ttgatgccga accatagcct gaaaggctgc ctccagggaa ctttccggaa
      481 tcggaatggg tctctcaatg gcagggacag ttccccacta acaattatga tttctatcct
      541 tacggcggaa tacataggaa tgtctgtttg tactgtattc cgaagacatg tatagaggat
      601 gtttcaataa aaacggatat aaaaccgggt gctgcagaaa tatccttcgt tacaaaaatt
      661 aataagcctt tagcttcaga tatcagggta tatttgaaca accgattaac aggagaatac
      721 aaattgaatg gcagtgatga aataacaggt agcgttttgg tggaggagcc tgtgttatgg
      781 gacatggaca acccgtattt gtataatttt aaagtagtac tgcatgaaga aatattaatg
      841 aagataaata ctggaaggtg cctgatacta tccatgggtt tggtggaggc gaaaatgaga
      901 caagagtaat attcagtatt gccgacgcca aggaaaaaat caaggtggac aaggcagagc
      961 accttattga aagcggctac aacgattatc tgaaggcact gattatattt tatatgatgt
     1021 attgatttat agaattccat aatatagtat taagtttata aggtggttcg aatatgtttg
     1081 tgtggtaaaa taaagagtgt atatactgtg tataaatgtt aatttatatg ttaacatcaa
     1141 ctgtaggttg ataattgtat agcagaccta aattgacatt ccattgtttt cttctgaaat
     1201 attagtaaaa taaaattaaa aagacgggag gaaatacaat gattaacaaa attgatgtag
     1261 gtaaaagaat tagcagtttg cgcataaatt taagctattc acaggcggaa tttgctgaaa
     1321 aattaagcgt aagtacacaa gctgtgtcaa aatgggaaac aggtcgtaca ttaccggata
     1381 ttgatgttct gttaaatata tcatggatat gtaaaacttc catcaacaat attttggagg
     1441 gcgatgattt tattgagtac aataccggca ttgacagagg ccttttacga ttaaacaaat
     1501 ttttaatatg tcctgaatgt aaaaaaaagc ttaagctgaa atctctcttt tttgaatgcg
     1561 aaaacaaaca tcaataccat attattgatg gagtagttga ttttaggacg agagagattc
     1621 cgggcgaaca atggtccttg agttatcgga attatgatga atatctgcac gagcatcatt
     1681 ggcctggaaa tccaaattat aaaagaggtt tgaatcctgc tgatgttata tggacagaga
     1741 tagaggagct tcgtcctaga ctgatattag atatggcttg tggaacaggg caaggaataa
     1801 agcatcagat aaaaagaata aactggccgg taacaatagt tatggtagat ttaagtcacc
     1861 gcattttaaa atgggataaa gttttttact caactgaatg ggaaaatccc tatgtagaaa
     1921 tggtctatct ggcttgtgac ggagcaaatt tacctatatt gagtgattct attgatgttg
     1981 tattttctta tgcaggttat gaaagcatgc agactaaaat gatggatggg tttcgtgagg
     2041 cgttcagagt tataaaagat ggtggacata caatttatac aaaatcagtt gtggaggatc
     2101 atgagagcga aaactctaaa aagtggacta acttattgtt ttctaatttg gatcatgacg
     2161 aacaatttgg gatgaaagat atgatgctgg atgttaatca atggcttcag acttgtaaag
     2221 atataggatt cattgaaaac aacagtataa aaatctacga cgaacttcca gtgccagaca
     2281 cagataagtt tccttttgaa aatgaaatag ctcaatggat ggcggaatat gtttttaatt
     2341 ctaggaagcc ttgatgtaca gtatagaaat aatatagagt ttagttattc gcacatttta
     2401 tacactttgt ataacattat tataatctga acttaaagtt atgaatatta tagggagatt
     2461 attttgcgaa tcacattgca gacatatttc aaaggaagta tcgaagcagt ttagctttac
     2521 cagaaagcct ttgatgctat acttggttat tgtatgctaa attttggtaa tacataaatg
     2581 cacgctgaga taaatttgaa tgagcagaat attgtttcaa taagtaagtc aaatgattgg
     2641 gcagttacac gtagaaatat acagttctgt attaccttac tgccgtattg aagatgtccg
     2701 ttttcaggaa ggcgaaactt gtgatgcgat gtgggcaagc acagataaaa tccgtgaaat
     2761 gatggcttct ggtgagtttc tcagcgaatg gttctatcca tattttgaag agatggtgga
     2821 gaaatggggg gtgatagtat gttaatccgt tgggcggccg aaaaagacct gcctgcatgg
     2881 tatgcactgg caaccgaggt ttcacagatt ttccagcacc caggcaacat gggaaccgac
     2941 cctgagtttg cttcatatgc acaaagcgaa gtgagtaaat acgaagtatt gaccgccgta
     3001 gactacatga gtggcaagaa tatggggttc atcgagcaga tgagaaagga gttgcgacat
     3061 gcctaaactg ccaaatacac tagtcatcct gccctacgac ccaaattgga aaactgaatt
     3121 cgagcgcatc cgcgattatc tgatggagca tatcggcgac ctggtcattg aggttaaaca
     3181 tatcggtagc acttcggtgc ctggcttgtg tgccaagccc atcattgatc ttgtcgcagt
     3241 gatggaatcc tacgaggtgt tccctcaaat tgtgtcccgt ctcgaaaaag tcggttttca
     3301 gcatgagggc gatttaggca taaaagaacg cgaggttttc aaaaggataa tccctgatga
     3361 ctttatggat tatcactttt atgtatatcc gaaagatagc gaggaaaacc gcagacaaac
     3421 cagctttcga aatgcgcttc ttaagaatcc tcagattgcg gaggaatacg gcaagttgaa
     3481 aacaaggcta atcagcgagg tgaacggcga ccgtgttctt tacactaact caaaaaccga
     3541 ctttatcttg gacgtgatca accgtgttaa tgataatggc gagttgaaaa acatttcgca
     3601 aaacatatat gataaccaaa ccttttttga cggatataaa aaattacgtg aaaatccgga
     3661 taatgctaat ctgttggagg aaaagcctgc actcttttcg ctcgcgccgg atttgaccag
     3721 cctttctgtt ctcgatttag gctgtggcta cggagaaaat tgtgctgaat ttctgcggct
     3781 tggtgccgct aaagtggttg gagtagatat atcttcaaaa atgcttgagg ttgcaaaaca
     3841 tgaaacaagt ggggttgaat atatttatgc tgatatgagc gatttgtctg ctatagaagg
     3901 aatttttgat gtcgtgttca gttcgctcgc aattcattat atcgaggatt ttagtaaact
     3961 ttgttaccaa gtttttgact tgttgaattc ctacggttat tttatctttt cgcaggaaca
     4021 cccatttact accgcgccat taaaaggt
//
LOCUS       DAOSDD010000327         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7860_length_4032_cov_5.106110, whole genome shotgun sequence.
ACCESSION   DAOSDD010000327 DAOSDD010000000
VERSION     DAOSDD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7860_length_4032_cov_5.106110"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK733_18445"
     CDS             <1..209
                     /locus_tag="PK733_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12549.1"
                     /translation="KSVPFTHSMELYIQLSNKYPGSRVFLDIFDGGHELRMEDAKRWF
                     LSQINDNNNTEGDNKSNNRSCLTK"
     gene            559..1629
                     /locus_tag="PK733_18450"
     CDS             559..1629
                     /locus_tag="PK733_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOJ12550.1"
                     /translation="MHKKLFIPGPVEVSDDVLQKMSTPMIGHRTKEASALQRRISEKM
                     MKVMYTQNQILLSTTSGSGLMEGAVRSCTRKKAAIFSIGAFGDRWYKMAASNGVPADK
                     FSSEQGKTTTPEMIEKALSTGEYDLITITHNETSSGVMNPVEELSQVWKRYPEVVVCM
                     DTVSSMGGTKIEIDKLGVDICITSSQKCLGLPPGMAICSFSQKAVEAARNVKYRGLYL
                     DLLDLYEYIQKKDYQYPSTPSLSHMFALDYQLDKILEEGLENRYARHLEMAQYTREWA
                     KANFALFPEEKFASNTLTAVKNIRNINVGELNKRLGEKGYMISNGYGDLKEVTFRIAH
                     MADTTLAEIKELLSLIDEIIGQ"
     gene            1791..1866
                     /locus_tag="PK733_18455"
     tRNA            1791..1866
                     /locus_tag="PK733_18455"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1824..1826,aa:Trp,seq:cca)
     gene            2755..3660
                     /locus_tag="PK733_18460"
     CDS             2755..3660
                     /locus_tag="PK733_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ12551.1"
                     /translation="MDWITGLGRAIDYIEEHITEEIDYNEVAKQAYSSSYHFQRVFSI
                     LCGYTLGEYIRSRRLTLAGDELSGTSDKVINIALKYGYDSPDGFTRAFTRFHGISPSL
                     ARETGAVLKSFSRLQLKLYLEGGNTMNYRIEEKEARIYTGYARHFNGVPGNRGKQEED
                     FFIHTRSNQYLLQGMADDSSAIYNIITNIDDEGYDFYIAALLPDYMREHIEDDGVLGK
                     EDAKRFEMIEIPRTTYAVFETERVKFPTKQHIELRRRIVTEWLPSSGYVLAEGAEITL
                     THWYKKPHHDKRYIEIWLPVMKKSE"
BASE COUNT         1317 a          677 c          837 g         1201 t
ORIGIN      
        1 ataagtccgt accatttact catagtatgg agctttacat tcaattaagc aataaatatc
       61 ccggttcaag ggttttcctg gatatttttg atggcggaca tgaattgagg atggaggatg
      121 caaaaagatg gttcttatct caaattaacg ataataacaa cactgaggga gataacaaaa
      181 gcaataacag aagctgcctg actaaataag ttattacact taaggctgca aggattttat
      241 agtttacttt ttattaggtt atgttataat aaatacaatc aaggaaataa aaccttgtgg
      301 ttttacgttt ttgtatttat tgaaacggtg tgaagcaatc cgattagcgg attgcactgt
      361 tgttataata aataccatca gagaaataaa accttgtggt tttatgtttt tgtaattatt
      421 gaaacggtgt gaagcaatcc gattagcgga ttgcgctgtt gtataataat attattttag
      481 aagcaacttt tttacaatat gctatgttat aaaattaaca ataaaaccag gaagattgta
      541 taaggaggta taaaattagt gcacaaaaaa ttatttattc cagggcctgt tgaagtttcc
      601 gacgatgtcc tccagaaaat gtctacacct atgattggac accgtacaaa agaagcttca
      661 gctttacaga gaagaatatc agaaaaaatg atgaaggtta tgtatacaca aaatcagatt
      721 cttttgtcaa ctacatctgg aagcggttta atggagggag ctgtcaggtc ctgtacaaga
      781 aaaaaggcag caattttctc aataggagct tttggtgaca gatggtataa aatggctgct
      841 tcaaatggtg ttcctgcaga taaattttct tctgaacagg gaaagactac aacacccgaa
      901 atgattgaaa aggctttgtc tacgggtgaa tatgatctta taacaattac acacaatgag
      961 acatcttcag gagttatgaa tcctgtagaa gagttgtccc aggtatggaa aaggtatcct
     1021 gaagtggttg tttgcatgga tactgtaagt tcaatgggtg gtacaaaaat tgaaattgac
     1081 aagcttggtg ttgatatttg tattacatca agccaaaaat gtctggggct tcctccggga
     1141 atggcaattt gctcattttc acaaaaggct gtagaagctg caaggaatgt caaatatagg
     1201 ggtttatacc ttgatctgct tgatttgtat gagtatattc agaaaaaaga ctaccagtat
     1261 ccatctacac cttcattgtc acatatgttc gcactggact atcaattgga taagattctt
     1321 gaggagggac tggaaaacag gtatgcacgt caccttgaaa tggcgcaata tacacgtgag
     1381 tgggcaaaag ctaatttcgc tcttttccct gaggaaaaat ttgcttcgaa cacgctaaca
     1441 gctgttaaaa acatcagaaa tataaacgtt ggtgagttga ataagcgttt aggtgaaaaa
     1501 ggctatatga tttcaaatgg atatggcgat ctgaaggaag taacttttag aattgcgcat
     1561 atggccgata cgacattagc tgaaattaag gaattgttat cattgataga tgaaattata
     1621 gggcagtaaa attgattaaa agggttgcag cgaaaaggta taatactgta accctttgca
     1681 ttgcaaaata atgatttatt gtgtatacct gatcgctcta atagcagaaa tttttagaaa
     1741 ttaccattga cacactagaa aacatattgt atactaaact agtcagttga aggggagtag
     1801 ctcaattggt agagtagcgg tctccaaaac cgtcggctgc aggttcgagt cctgtctccc
     1861 ctgccataga gaagcctaga ggcataatgc ttttaagctt ctctattttt ttactaaact
     1921 ataataaata tcaaaatggg gattattagg gggctatagc attctataat acctctcgtt
     1981 gtaaagacag attttataaa ttttatgtca gattatcctt tccataattt gtatatactt
     2041 aaatattatg cactgcagtt aagaatgtca agggcgagcg gtttttgctc attcatcgga
     2101 ggcgaaggcg gagacccttg acaactattg ccgttccatc ctgttgttct ccggtctgtt
     2161 atgacagacc ggctgccatc tagcgaggac ggggtatggg gcagtcccaa ggtcttattt
     2221 cgcttcagga agtagtccgt tacatacaaa gtagcatttt gattgagtct tgtaatctaa
     2281 ggattgatgt ggtctctccg tgttgtaata ctaacatatt cttttactat cttgcggagt
     2341 ttatgatatg tactatacaa ttattatgcc cttcaaacac tttaatttca ataatttgaa
     2401 ggacttttat tgtaatttac cgaaactcac gctagacaaa ataaaaaatc cacttgacga
     2461 cattaatgga taattataca atgtacttat ataaattcta cttcttctac gcaatgcacc
     2521 taagttttat gattttcttc ttagatgcat tgcgtgaaag aagtagttat ctgtaaatgc
     2581 gcgcccggtc ctctggacga cgggcggaat taaaacattt tgtcaaagaa agatttatag
     2641 tggcaagtag attatttata taatatatgt ttattactga tatcatgaag caaaaaaaat
     2701 cgggaaaaag catacacctt caattataat gtgtttgaga aaggcggtta gaatgtggat
     2761 tggattacgg gattaggtag agcaatcgat tatattgaag agcatattac cgaggagatt
     2821 gattataacg aggttgcaaa gcaggcttat tcgtcaagct atcattttca acgagtattt
     2881 tctatactgt gtggatatac attaggcgaa tatatccgta gtcgcagatt aactcttgct
     2941 ggtgatgaat tatctggcac aagcgataag gtgataaata tcgcattgaa atatggatat
     3001 gatagccctg acggctttac acgagctttt acccgttttc atggaatcag cccttcttta
     3061 gcgcgcgaga caggtgctgt tcttaaatct ttttcacgcc ttcaactcaa attatatttg
     3121 gaaggtggta atacaatgaa ttatagaatt gaggaaaaag aagctcgaat ttatactgga
     3181 tatgcccgtc attttaatgg tgtcccaggc aatagaggaa aacaggagga agatttcttc
     3241 atacacactc gttcaaatca atatctctta caaggaatgg cagatgacag ctcagcaatt
     3301 tacaacatta taacaaatat tgatgatgag ggttacgatt tctacattgc tgcattgtta
     3361 ccagactata tgcgtgagca cattgaagat gatggcgtat taggcaaaga agatgctaag
     3421 cgttttgaaa tgatcgaaat acccagaacc acatatgctg tatttgaaac tgagcgggta
     3481 aaatttccta ctaaacaaca cattgagctc cgaagaagaa ttgtaacgga atggctcccc
     3541 tcctccggct atgtactggc tgaaggagcg gaaatcacac ttacacactg gtataaaaag
     3601 ccacatcatg acaaacgata cattgagata tggcttccag taatgaaaaa atcagaataa
     3661 aatctaatat gaaggtagtt accccaaaag ttagtcctct atgaacgcag cagacgcttt
     3721 tctatatatt gaaaagagaa tattataaac atttaaacaa ttggatattt gaaaaatata
     3781 aagtttatgt ccagccttga ctccgccgtg cagctggaca tgatggggca cacatcagat
     3841 aacagcggca ttcatgcaga gggttcgacc aaaacctgcc agaggtcaag cttcttcgta
     3901 gcctcaccac atgcccgagg ctaagccgaa gtgtcgctaa gcctcggcca cgtcatgaat
     3961 gcgcaaaccg ttacacgtaa tcaggtgcaa ggagcgccat ccatggcgcg atttagtaag
     4021 aaaggctttg ta
//
LOCUS       DAOSDD010000328         4004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7925_length_4004_cov_4.621423, whole genome shotgun sequence.
ACCESSION   DAOSDD010000328 DAOSDD010000000
VERSION     DAOSDD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7925_length_4004_cov_4.621423"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PK733_18465"
     CDS             <1..1213
                     /locus_tag="PK733_18465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOJ12552.1"
                     /translation="IYNVGVDCAILMNPQVWIASGHVGGFNDPLIDCKTCKSRQRADK
                     MIEEWNREQGIDISVDGWDNQALVQYIKDKGVTCPECGASNFTDIRKFNMMFKTFQGV
                     TEDSKAEIYLRPETAQGIFVNYKNVQRTTRRKIPFGICQIGKSFRNEITPGNFTFRTR
                     EFEQMELEFFCEPGTGLKWFKYWKDYSYNWLLSLGLKPETLRMRDHSKEELSFYSNAT
                     TDIEYLFPFGWGELWGIADRTDYDLKQHMEHSGDDLSYFDPVTNQKYIPHCVEPSLGA
                     DRVTLAFLCEAYDEEELAEGDVRTVLRFHPALAPVKIAVLPLSKKLGDEAFKVYETLI
                     KEYPCEYDETGSIGKRYRRQDEIGTPYCITFDFDSLNDESVTIRERDTMEQVRIRISK
                     LSDYFNGKFNF"
     gene            1510..>4004
                     /gene="ppdK"
                     /locus_tag="PK733_18470"
     CDS             1510..>4004
                     /gene="ppdK"
                     /locus_tag="PK733_18470"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOJ12553.1"
                     /translation="MGKYVYLFTEGNASMRNLLGGKGANLAEMTSIGLPVPRGFTITT
                     EACTRYYIDGQIIAQKIQDEIFAALSKTETTVGKKFGDPENPFLVSVRSGARASMPGM
                     MDTILNLGLNDTVVQGLAKLTDNPRFAYDSYRRFIQMFSDVVMEIEKSKFEKILDRVK
                     EKNGAKIDTDLTAENLKEVVSMYKELFKAEKGFDFPQDPKEQLMEAVKAVFRSWDNPR
                     AIVYRRLNDIPGDWGTAVNVQEMVYGNMGTDSGTGVAFTRNPSTGEKKLYGEFLMNAQ
                     GEDVVAGIRTPQSIELLKEINQEAYNQFVSTADILEKHYNDMQDMEFTIEKGKLFMLQ
                     TRNGKRTAAAALRIAVDLVNEGMVTKEEALMKVDPKQLDALLHPTFEPLALKKATPIA
                     KGLPASPGAGTGRIYFHAQDAVNAYNNGEKNVILVRLETSPEDIEGMNVAQGILTGRG
                     GMTSHAAVVARGMGKCCVAGCSEVKIDNEAKYFIDKNGKRYEEGEWISLDGSTGNVYG
                     TKLPTVDPELTGDFSKLMGWADDVRMLKVRANADTPKDAAQALKFGAEGIGLCRTEHM
                     FFEADRISTFRQMIVADSEEKRRAALDKLLPMQREDFEGIFTEMNGYPVTIRLLDPPL
                     HEFMPEKEEDVVLLADKLCITYDELKAIIKRQHEINPMMGLRGVRLAVIYSEIAEIQT
                     RAIIEAAINVNKKGMTVIPEIMVPLVCEVKEFQFVKNIIVKTADEIIKNSGVDLKYMV
                     GTMMEIPRAVLTADEIASEAEFFSFGTNDLTQMTYGLSRDDAGRILDVYYQKKIFEYD
                     PTARLDQVGVGKLVKMGVELGRSSRPDIKLGVCG"
BASE COUNT         1368 a          600 c          915 g         1121 t
ORIGIN      
        1 tatttataat gttggtgtgg attgtgcaat attaatgaat cctcaggttt ggattgcttc
       61 gggacatgta ggcggtttca atgacccgct tatagattgc aaaacatgta aatcacgtca
      121 aagagcagat aaaatgattg aagaatggaa tagagagcaa ggaattgata tttcagttga
      181 tggctgggat aatcaggctt tggtccagta cataaaagat aaaggtgtaa cctgtccaga
      241 atgcggagca agcaatttta cagatatcag aaaatttaat atgatgttta agacctttca
      301 gggtgttact gaagattcca aggctgaaat ttacttgaga ccggagactg cccagggtat
      361 atttgttaat tataaaaatg ttcaaagaac tacaagaaga aaaatacctt ttggaatatg
      421 ccagataggt aagtctttca ggaatgagat aacccctggt aatttcactt tcagaacaag
      481 agaatttgag caaatggagc tggaattttt ctgtgaaccc gggacaggcc ttaaatggtt
      541 taaatattgg aaggattatt catacaactg gcttttaagt ctgggtttaa aacctgaaac
      601 tttaagaatg agagatcata gtaaagaaga actttcattc tatagtaatg caactacaga
      661 tatagaatat ttgttcccgt ttggttgggg tgagctttgg ggaatagctg acagaaccga
      721 ttatgatctt aaacagcata tggagcattc gggagatgat ctttcgtatt ttgatcctgt
      781 aacaaatcaa aaatatatac cccactgtgt agaaccatcc ttaggtgccg acagggtaac
      841 tcttgcattt ctctgtgaag cttatgacga agaagagctt gcagaaggag atgtaagaac
      901 tgttttacgt tttcatcctg ctcttgcacc tgtaaaaatt gcagtactgc cattatccaa
      961 aaaattgggg gacgaggcat tcaaggtgta tgaaacactt ataaaagagt atccctgtga
     1021 atacgatgag acaggaagca taggtaaaag atacaggagg caggatgaga ttggaacacc
     1081 ctactgtata acttttgatt ttgattctct taacgatgaa agtgtaacta taagagaaag
     1141 ggataccatg gaacaggtaa gaattagaat aagcaagctt tctgattatt ttaatggaaa
     1201 attcaacttc tagccttact ttattcaaag tttttttttc gttatcttaa catatgtttt
     1261 aaaaatatgg ataaaataac ataataaata tgtaaaaaat aaatagcaca ataattaaaa
     1321 taaaaagcaa aataaataat gacataaata atgacataat taataataca atgaattata
     1381 ctggtattgt attgtcaaat gtatacaata tgcaatataa ttttaattga agtatagtcc
     1441 ctgcactgta gtgccggtgt catttaatta tatactgttt tacaaaacta aataatggag
     1501 gtagcacata tgggaaaata tgtatactta tttactgaag gaaatgcttc aatgagaaat
     1561 cttcttggag ggaaaggtgc caaccttgca gaaatgacaa gtataggact gcctgtaccg
     1621 agaggattca ctataacaac agaggcttgt acaagatact acattgatgg acagattatt
     1681 gcacagaaaa tacaggatga aatttttgca gcgttgtcta aaactgaaac aactgttggg
     1741 aaaaaatttg gtgacccgga aaatcccttt ttggtgtctg ttcgctcagg ggcgagggca
     1801 tcaatgcctg gtatgatgga tacaatattg aacctgggac ttaatgacac tgttgttcag
     1861 ggactagcaa aacttaccga taatcccaga tttgcttatg acagttacag acgttttata
     1921 cagatgttta gtgatgttgt tatggagatt gagaagagca agttcgagaa aatactggat
     1981 cgagtaaaag agaaaaacgg tgcaaagata gacaccgatt taactgctga aaacctcaaa
     2041 gaggttgtca gtatgtataa ggaattgttt aaagcagaaa aagggtttga cttccctcag
     2101 gatccaaaag aacagcttat ggaagcagta aaggctgttt tccgttcatg ggataaccct
     2161 cgagcaatag tctacaggag gcttaatgat attcctggtg attggggaac ggcagttaat
     2221 gtacaggaaa tggtttatgg aaatatgggt accgattcag gtacaggagt tgcttttaca
     2281 agaaatcctt ctactggtga gaagaagctt tacggggagt ttcttatgaa tgcccagggc
     2341 gaggatgttg ttgcaggtat caggacaccg caatctatag aacttcttaa ggaaataaat
     2401 caggaagcat ataaccagtt tgtttcaact gcagatattt tggaaaaaca ctataatgat
     2461 atgcaggaca tggaatttac catagaaaaa gggaagcttt tcatgcttca gacaagaaat
     2521 ggcaagagga ctgcagcagc ggcattaaga attgcggtgg atctggttaa tgaaggtatg
     2581 gtaacaaaag aagaagccct tatgaaggtt gatccaaaac agctggatgc tctattgcat
     2641 ccaacctttg aaccgttggc actaaagaag gctacaccta tagctaaagg acttcctgct
     2701 tcacctggtg cgggaacagg caggatatat tttcacgctc aggatgctgt caatgcatat
     2761 aataacggtg aaaagaatgt tattcttgta agattggaga catcaccaga agatattgag
     2821 ggaatgaatg ttgctcaggg aatcctgacc ggcaggggcg gaatgacttc acatgcagcg
     2881 gttgtagcga gaggtatggg caaatgttgc gtagcaggtt gtagtgaagt aaaaatagat
     2941 aacgaagcga aatattttat agataaaaat ggtaagagat atgaagaagg agaatggata
     3001 tctcttgatg gttccacagg aaatgtatat ggcacaaagc ttccaacggt tgatcctgaa
     3061 cttacaggtg atttttctaa attaatgggt tgggccgacg atgtaagaat gttgaaagta
     3121 agagcaaatg cagatacacc aaaggatgct gcacaggcct tgaagttcgg tgctgaaggc
     3181 ataggccttt gccgtactga gcatatgttc tttgaagctg acagaatctc tacattcagg
     3241 caaatgattg ttgcagacag tgaggaaaag agaagggctg ctcttgataa gttgctgccc
     3301 atgcaaagag aagattttga aggcatattt actgaaatga acggctaccc cgtaacaata
     3361 aggctgcttg atccaccttt gcatgaattc atgccagaaa aggaagagga tgtagtttta
     3421 ttagcagata aattatgcat cacttatgat gaattgaaag caataatcaa aagacagcat
     3481 gaaatcaatc caatgatggg tttaagaggc gtacgccttg cagtcatata ctctgagatt
     3541 gctgaaatac agacaagagc aattatcgaa gctgcaatca atgttaataa aaaaggtatg
     3601 actgtaattc ctgaaataat ggtgccactt gtatgtgaag ttaaggaatt tcagtttgta
     3661 aagaatataa ttgtaaagac agccgatgaa attattaaaa attcaggtgt tgaccttaaa
     3721 tatatggttg gtacaatgat ggaaattcca agagctgtcc ttacagctga tgaaattgct
     3781 tctgaagctg agtttttctc tttcggtacc aatgacctta ctcagatgac ctatggttta
     3841 agccgtgacg atgctggaag gattttggat gtatattatc aaaagaaaat atttgaatat
     3901 gacccaacag ccagactcga tcaggttgga gtaggtaaac tggtaaaaat gggagttgaa
     3961 ttgggacgta gttcaagacc cgatattaaa ctcggtgtgt gtgg
//
LOCUS       DAOSDD010000329         4001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7936_length_4001_cov_5.679422, whole genome shotgun sequence.
ACCESSION   DAOSDD010000329 DAOSDD010000000
VERSION     DAOSDD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_4001_cov_5.679422"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..367)
                     /locus_tag="PK733_18475"
     CDS             complement(86..367)
                     /locus_tag="PK733_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOJ12554.1"
                     /translation="MIIGTIIIKFYTSWVHSLKEKRMVVKSICVKVRNKFNVSIAEIG
                     EQDTHKTIILGVACVAGETSQADSILDNVLNFIENSTDAEIIDIQREIR"
     gene            complement(436..2433)
                     /locus_tag="PK733_18480"
     CDS             complement(436..2433)
                     /locus_tag="PK733_18480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ12555.1"
                     /translation="MSLLVLNNIVKEYRNRVVLNGASLRIEKGERVALIGANGSGKTT
                     LLRIAAGMESSDGGSVIIARGTKMGYLTQDLREMEPTGKIFSETALYHEEISRLEHKI
                     RELEKKMAEPELMNTPEEYNALVSEYSRLVNRFESLDGYTIDLKIKSMLLGLGLKEEA
                     LTLPLELLSGGEKMRVALARILLEEPDLLVLDEPTNHLDIDAAEWLEGFLKRFNGGVL
                     VVSHDRYFLDQVATRVAELGNGTITERSGNYSTFIEQKDIMSEFARKEQFRLKMEIRH
                     ETAIAEQLKATSKISAWKSRLKTVQRLEKELTVGLNEMKERYNLYRTAAPKISFDKIK
                     HMSAEIAVAEGLEKYYSEYRLFSGVNFLIKGGEKIGIIGPNGCGKTTLINILLGKDQN
                     YKGTAKLGEWVRYGYLGQVVNFKDEKRTIMEELLAAKEMPVDAARDYLAKFQFYGDEI
                     DKKIEVLSGGERVRLYLGCMMLENPDCLIMDEPTNHLDVPARDALESALMKYNGTIIA
                     ISHDRYFLNRCVNRILEFSDGTARLFNGNYDAYRENKAENETLRKTKESKGNITAIKN
                     PDKSAVSKSLDRPAVTKSLGRSGTKKACNISGRNITRQQVEGKLNITEIEARIIELEE
                     KRKELESTFGIDTPPEKYVEYDKVIKEIECLYELYEESSNA"
     gene            complement(3305..>4001)
                     /locus_tag="PK733_18485"
     CDS             complement(3305..>4001)
                     /locus_tag="PK733_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12556.1"
                     /translation="YFKNVKDTVPQKKLDEFFEKIEKGEKNIRIQLEDDVRLSRKEGS
                     GKSETYYIEYFGNDQRKIGQYSKYPMLMNLEIFKESMLENLTIIGFGYKWDKEVHFSI
                     KNRDTIGTNTNKPSANSFIDTIARIDARVLDSRLKQYPAFDIVIDDYVSCMNSEVRDE
                     ELIFDKNNHPYVVTGAKSIDAKTGKETIYGRYFRTVKEAAGDYVHYNIIIPETMLKGM
                     SDQEFIDGLGFIE"
BASE COUNT         1106 a          908 c          574 g         1413 t
ORIGIN      
        1 ctatgataca aaatatggca gcatacgatt tcccgagtat aaaatttgaa caaagtttac
       61 ggatgcggtt attgcctcaa gtaatttacc tgatttcacg ctgtatatct ataatttctg
      121 catctgtgct gttctcaata aagttgagca cattatccaa tatactgtca gcctgacttg
      181 tttcacccgc tacacaagct acccccaata taattgtctt atgagtatcc tgctcaccta
      241 tctcggcgat agatacattg aacttgtttc ttactttgac acatatgctt tttaccacca
      301 tgcgtttttc cttgagggaa tgcacccagg atgtgtaaaa tttaataatt attgttccga
      361 taatcatatt gttctccttt cagcagagca aatgttttct gtctcctatt gcccgctata
      421 ttgatcaagc attacttaag cattgctact ctcttcatat aattcataca agcattcaat
      481 ttcttttatc actttatcat actcaacata tttttcagga ggagtatcta ttccaaaagt
      541 actttccaac tccttgcgtt tttcctcaag ttcaatgatc ctagcttcta tctctgttat
      601 attcagcttt ccctcaacct gctgcctggt tatatttctg ccggatatat tgcaagcttt
      661 ctttgtgcca gacctaccca aacttttagt tacagcaggc ctatccagac ttttgcttac
      721 agcagatttg tcaggatttt tgattgctgt aatattcccc ttactttcct ttgtttttct
      781 taaagtctcg ttttcagcct tgttttctct atatgcatca taattcccgt taaaaagtct
      841 ggcagtacca tcggaaaatt ccagtattct gttaacacac cggttcagga agtaacggtc
      901 atgggaaata gctataatag taccattata cttcatcaat gcagattcca gagcgtccct
      961 tgcaggtaca tcaagatgat ttgttggttc atccataatt aagcagtccg gattttcaag
     1021 catcatacac cccaggtaca gtctcaccct ttctcctccg cttagaactt caatcttctt
     1081 atcaatctca tccccgtaga attgaaattt ggcaagataa tcccttgcag catccacagg
     1141 catctctttt gcagcaagca gttcttccat tattgtgcgt ttttcatcct taaaattcac
     1201 tacctggcct agatacccat atctaaccca ttctcccagt ttggctgttc ctttataatt
     1261 ctgatcttta ccaagaagaa tatttatcag agtagtttta ccacaaccat taggacctat
     1321 tatacctatc ttctcccctc ccttaataag gaagttgaca cccgagaata gccggtattc
     1381 actgtaatat ttttccagtc cttcggccac agcgatttca gcactcatat gtttaatttt
     1441 atcaaaagat atctttggtg ctgctgtccg gtaaaggtta taccgctctt tcatctcatt
     1501 gagccctact gtcaattcct tttcaagccg ctgtacagtt ttcagtctgc ttttccaggc
     1561 gcttatcttg cttgttgcct tcaattgttc agcaatagca gtttcatgtc ttatttccat
     1621 tttaagtcta aactgttcct tacgtgcgaa ctcactcatt atgtcctttt gttcaataaa
     1681 agtagaataa tttccacttc tttctgtaat tgttccgttt cccaattcag caactctggt
     1741 agctacttga tcaagaaagt atctatcatg ggatactacc aggacacctc cgttaaatct
     1801 tttcaaaaat ccttccagcc actctgctgc atctatatca agatgatttg ttggttcatc
     1861 cagcaccagc agatctggtt cttccagaag aatccttgca agtgcaactc tcatcttctc
     1921 tccaccgctt aataattcaa gcggaagggt aagtgcttct tctttaaggc ccaggccaag
     1981 cagcatagat tttattttca aatcaatagt atatccatca agactttcaa acctgtttac
     2041 aagccttgag tattctgaaa caagagcatt atattcttcc ggagtattca tcaattccgg
     2101 ctccgccatt tttttctcaa gctctcgtat tttgtgttcc agtctgctga tctcttcatg
     2161 ataaagcgca gtttcactga atatctttcc agtgggttcc atttccctta aatcctgagt
     2221 tagatacccc atctttgtac ctcgtgcaat tattacactt ccaccgtcgg aagattccat
     2281 acctgctgct attcttaaaa gagtggtctt tcctgaacca ttcgctccaa taagagcaac
     2341 cctttcacct ttttcaattc tcaggctggc cccattaagc actaccctgt tccgatattc
     2401 ttttacaatg ttatttagca ctaataaact cataagtttt tcctcctttt ttataaccgg
     2461 aatattccat acatgtgtat tgtgcagata tatccgcggc tatcattccg gcaggaaata
     2521 catataatgt tcaccaaata gacaatcttt taatccttta cctgggaaaa atagaaaata
     2581 taaaagccgc ggaattcggg tgccgtccgc agcttaataa cctagttatc taaatagcat
     2641 agctgaaatt ttaaatttag aactcaaaag ctcatgcaaa aacctaataa ttcattaaag
     2701 ttcatgcatt ttatagtttt ttcaccaata cagccggatt ttccttgaaa atgtgatgat
     2761 atattcaaca aaaaagtaca acagaataca atagatatac aacaattata actgtagatt
     2821 gttgattaca gcaagctgtt aaatttattc aaatcatccc ggcttcggct acttctggct
     2881 tttaggctct ggacaacgca tcaccgaatt aaatatgcac tttggtcctc tgactctatg
     2941 gtcattgtac agagctaagg ccgcggcctt ttgccggtac attgagaaaa caccattcca
     3001 tggttagatc ctcctgtacc agttaattat aaataattta tatagaatgg tgcctggcac
     3061 cattctatac gtttattcct atatgagtta tattatagta tatgcatgta gcaaaaatca
     3121 ataccattct atatagagac atttccccgt gcgacatctt cccgatgcta tcaaaaatta
     3181 tcccggaaga catcgctttc caaattgaca tagtagtatt aaacctatcc ggtaaacatg
     3241 ctgtaacttt aattatcctt tatttatagt gatttttatg atattgaaca ccaagaatgc
     3301 ttttttactc aataaaacca agaccatcta taaattcctg gtcactcata cctttaagca
     3361 tagtttcggg tataataata ttataatgca catagtctcc agctgcttct tttactgttc
     3421 tgaagtacct tccatatata gtttctttac ccgtttttgc atctatcgac ttagctccag
     3481 ttacaacata tggatggtta tttttatcaa atatcagctc ttcatcccta acctcactat
     3541 tcatgcaact gacataatca tcaattacaa tatcaaaagc cggatattgt ttcagcctgg
     3601 aatcaagaac tcttgcatct atccttgcaa ttgtgtcaat aaacgaatta gcagaaggtt
     3661 tgtttgtatt agtcccaatt gtatcacgat tttttataga aaaatgaact tccttatccc
     3721 atttataacc aaagcctatt atagtcagat tttccagcat actttccttg aaaatctcta
     3781 aattcataag catgggatat ttgctatatt gtcctatttt ccgctggtca ttgccaaaat
     3841 attctatata gtaagtttca cttttaccgc tgccttcttt tcttgataat cttacatcat
     3901 cttccagttg aattcgaatg tttttttcgc ctttttctat cttttcaaaa aattcatcca
     3961 gtttcttttg tggtactgta tcttttacat ttttgaagta t
//
LOCUS       DAOSDD010000330         3992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7957_length_3992_cov_5.091440, whole genome shotgun sequence.
ACCESSION   DAOSDD010000330 DAOSDD010000000
VERSION     DAOSDD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_3992_cov_5.091440"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1909)
                     /locus_tag="PK733_18490"
     CDS             complement(278..1909)
                     /locus_tag="PK733_18490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12557.1"
                     /translation="MRDFSTYFSVSGDYGCGGYSKPEFPELKDYLAHMDRLGISYSIV
                     SNTESRDYSVYHGNKRLLQDIDSCNAARDRIFPALTISPAMYYENGAMDFLKQCFETG
                     RAKVLKIFPSVSRFEIFQIEQVLKDVAKYKPILIIDTRFLENKLSFKEIVELTKLFPE
                     INIICTQVVWGSLQSVMDFMWRCNNVYIDTSWLHVKSSIELIVKYFGPHRILYGCGPK
                     YHNGASIAALVHARLSDTAKEQIAYNNIMKLMGVQSLPKIANIFLESANNEKPFWNKF
                     KTGSRIEGIDVIDAHTHIGAPARGWMLEKVEIPEQVEDIISAMDRLGITRMIACPEEA
                     QFANPLRGNRKTEESIKAFKEYKNRIIGYLVFNPHYSQQLIPKLDNLFKSKFFRGFKI
                     LSDYWGVPVTDPRYNAIWEYADKYHMPILIHTWDGNYSNPSLLKDITSKYPNAKFILG
                     HSGGSNKGRPEAEKLAINNPNVYLEWCGSFLCTIPWEETISRVGNKQIVFGTDTYFHE
                     PAWELGRLLSLDIADDELIPILGSNMKKILSEYRG"
     gene            complement(2104..3732)
                     /locus_tag="PK733_18495"
     CDS             complement(2104..3732)
                     /locus_tag="PK733_18495"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12558.1"
                     /translation="MKKHRIISLLLICLMIVGSFTACARDASKENADQKEKISEVHKG
                     EDKKNSESKFDKKIKFSATSFDVGEGEDYKSDALYKTLSEKYNFEIEFIPLSWDNWVE
                     RDRIWINSGDMPDVMFWNFNFVDYKNYTSQGLIKQLPADYESKYPNLAKSINRSGIAP
                     YLKQKMDEKLFTIPHVIYLTPITEDAIDAQAVYYRQDWAKKVGMSIGDMVTIEEITDL
                     AKAFIEKDPGENGAGKTIGISSSASGLYGMFVKVFNTYYSSFHKVDGKYVWGPFEEST
                     LEGIKVFSKYFKEGVFDKDYFTIKGKEFNDKFYAGKTGIFVEGCSADNLNGRKSNFAE
                     SNPTIDANEAFGICAVKGPNGKYNGSEMMNYWSASIFNPKIDDEKLDRILTLFDYIAT
                     PEGQKLINLGIEGKDYKTEGDKVTITREKDESGNFKRITKIHPSAGYFYTKVVLPDDW
                     SINDPSIPEITRNQVIKLFKEKQKIENLARNDYDLTFFSAPNKDKFNINVVDAITEII
                     LSGKDVEEAWKEWKKTVEPKVNPVLEEINNALAK"
BASE COUNT         1211 a          808 c          538 g         1435 t
ORIGIN      
        1 ggctttacca gatgggattc gcacccacta tatattaatc accgaacagg cgcacattca
       61 tataaaacac tcctcctatt cttccttttg ataattttaa ggtgtaacac cagttattcg
      121 tttggaggcc ctagcatctt ttgcacaaca tatagcaact aatatgttgt tttaagcagc
      181 aatgctctct atcctagaaa catctttacc tattataacc ctattagggc attaaacttt
      241 acttatcatg atattccaat tagtgatctg ttaatatcta acctctatat tcgctaagta
      301 tttttttcat attactacct aatataggta tcaactcatc atccgcaata tcaagtgata
      361 gtagtcttcc tagttcccag gcaggttcat gaaaatatgt atctgtacca aaaactattt
      421 gtttgttacc cactctagat atagtttcct cccaaggtat tgtacaaaga aaacttccac
      481 accattcaag ataaacatta ggattattta tcgcaagttt ttctgcttcc ggccttcctt
      541 tgttactacc gcctgaatgt cccagtataa actttgcatt ggggtacttt gaagttatat
      601 ctttaagtag tgaaggattg ctataatttc catcccaagt gtgtatcaaa ataggcatat
      661 ggtatttgtc agcatattcc catatagcat tatatcgggg atcggtaaca ggaacacccc
      721 agtaatcgga aagaatctta aagcctctaa aaaattttga cttaaataaa ttatcaagtt
      781 taggaattag ctgttgagaa tagtgtggat taaataccag ataaccaata atacggtttt
      841 tatattcctt aaaagcctta atactttctt cagtctttct attacctctt aaggggtttg
      901 caaattgcgc ctcttccgga caggctatca tccgagttat gcccaatctg tccattgcac
      961 ttataatatc ttccacctgt tcaggaattt ctaccttctc tagcatccat cctcttgcag
     1021 gggcacctat atgagtatga gcatcaatta catcaatacc ttcaatcctg ctgccggttt
     1081 taaacttatt ccagaatggt ttttcattgt tggcagattc caaaaatata tttgctattt
     1141 taggcaaact ctgcacaccc attaatttca tgatattatt atatgcgatc tgttctttgg
     1201 ctgtatcact taaacgtgcg tgaacaagag ctgcaatact agcaccattg tgatattttg
     1261 gaccacaccc ataaagtatc ctatgtggtc caaaatattt tacaattaat tcaatggaac
     1321 ttttgacatg aagccaggat gtatctatgt atacgttatt acatctccac ataaaatcca
     1381 taacagattg taaagaaccc cataccactt gtgtacatat aatatttatt tccggaaaca
     1441 atttggtcaa ttctacgatt tccttgaaac taagcttgtt ttccaagaat cttgtatcga
     1501 tgattaatat aggtttgtat tttgctacat ctttcagtac ttgttctatc tgaaatattt
     1561 cgaaccttga tacagatgga aatattttta atactttagc acgtccggtt tcaaagcact
     1621 gtttgaggaa gtccattgct ccattctcat aatacatagc aggtgatatc gtaagcgctg
     1681 gaaaaattct atctctagct gcattacaac tatctatatc ctgaagtagt ctcttatttc
     1741 catgatatac gctataatcc cggctttcag tattactgac aatagagtag gaaattccaa
     1801 gcctatccat atgcgccaaa tagtctttaa gttctggaaa ttccggttta gagtaacctc
     1861 cacacccata gtctccactt acactaaaat atgtggaaaa atctctcatt caatatgcct
     1921 ccttctttat gtgttttata ataacaaaac caaaataaaa gatagtccga tattaccctt
     1981 tttatcacca aaaataatca tactctgacc gatatcatta ataaaaatct ccaaagctat
     2041 aattaactat ctttggagac ttttctattt ggattaaaac attatggtta tttttctaga
     2101 tttctatttt gcaagggcat tgtttatttc ttcgagcaca ggattaacct ttggttcaac
     2161 agttttcttc cattctttcc atgcttcttc tacatcctta ccactcagga tgatttcagt
     2221 aattgcatca actacgttaa tattgaactt atccttattt ggtgcagaaa aaaatgttaa
     2281 gtcataatcg ttcctggcca aattttctat tttttgtttt tctttgaata gttttataac
     2341 ctgattacgt gtaatttcag gaatagaagg gtcattaatt gaccagtcat caggtaaaac
     2401 aacttttgta taaaaatatc ctgctgatgg atgtattttg gtaatacgtt taaaattacc
     2461 gctctcatct ttttctcttg taatagtaac tttatctcct tctgtcttgt aatctttccc
     2521 ctcaatacca agattaatta atttttgtcc ttcaggggtt gcgatgtaat caaatagagt
     2581 aagtattcta tcaagttttt catcatcaat tttgggatta aatattgaag ctgaccagta
     2641 attcatcatt tcactaccat tatacttacc attaggccct tttacagcgc atataccaaa
     2701 agcctcattt gcgtctattg ttgggttgga ttcagcaaaa ttagactttc tgccattaag
     2761 attatcagca gagcaaccct caacaaatat accagttttt cctgcataaa atttatcgtt
     2821 aaattctttt cctttaattg tgaaatagtc cttgtcaaat actccttctt taaaatactt
     2881 agaaaatact ttaattcctt ctaaagtaga ttcttcaaaa ggcccccata catatttacc
     2941 atccacttta tgaaagcttg aatagtaagt attgaacact ttaacaaaca ttccatacaa
     3001 tccgcttgca ctacttgaaa tacctatagt ctttccagcc ccattttctc ccggatcctt
     3061 ttcaataaaa gctttggcta aatctgttat ttcttctatt gttaccatat ctcctatact
     3121 catgccaacc tttttagccc aatcctgtct gtaataaacc gcttgagcat ctatagcatc
     3181 ttctgttatc ggtgtcaggt aaattacatg cggtatagta aaaagttttt catccatttt
     3241 ttgctttaaa tatggcgcaa tgcctgacct gttaattgat tttgcaagat ttggatattt
     3301 actctcataa tcagcaggca gttgctttat taacccctgg cttgtatagt tcttataatc
     3361 tacaaaatta aagttccaaa acattacatc cggcatatca ccagagttaa tccatattct
     3421 atccctttca acccaattat cccaactaag aggtataaac tcaatttcaa aattatactt
     3481 ctcagaaagt gttttgtaca acgcatcact tttataatct tccccttcac ctacatcaaa
     3541 gcttgtagca gaaaatttaa tttttttgtc aaacttactc tcactatttt tcttatcttc
     3601 tcctttatga acctctgata ttttttcttt ttgatcagca ttttctttac tggcatctct
     3661 ggcacatgcc gtaaaacttc ctacaatcat taagcaaata agtaatagtg atattattct
     3721 gtgttttttc atgataattc ctccctaact tttatttatt taaaggcata aaagccttta
     3781 gttccacttt atagttacaa ttattattaa taataactta tataatacta ctatgccaat
     3841 ttggaaaacg atatcttccg ggataatttt tgatagcatc gggaagatgt attccttaaa
     3901 ggccagcaga aattaccctc cggaaatgcg ctttccattg gcaacataac aaagtagtaa
     3961 taagttaata tcaaaatatc aatatctaag at
//
LOCUS       DAOSDD010000331         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_7989_length_3981_cov_4.839022, whole genome shotgun sequence.
ACCESSION   DAOSDD010000331 DAOSDD010000000
VERSION     DAOSDD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_3981_cov_4.839022"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            558..1646
                     /gene="ychF"
                     /locus_tag="PK733_18500"
     CDS             558..1646
                     /gene="ychF"
                     /locus_tag="PK733_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOJ12559.1"
                     /translation="MKIGLIGLPSVGKTTLFNLLTGSEMEVSDFSRSKIDATLGIAKI
                     PDERIDFLAKMYDPKKITYPTIEVVDVPGLVRGSSAGEGVGNQFLDNIRKTDALIHIL
                     RAFESESVVHAEGNIDPMRDIETINLELLFADLGIIENRIKRIETSKKVTKENLEELE
                     VLKKCREGLENGLLIHNLDLSDEEKEHLKTFAFLSEKPMILVINVDENQFMTGDYLSK
                     EALLAYTKQVNTPIIELCIKSELEISQLELEDRKMFMEDLGISESGVDKLAKTAYGYL
                     GLISFLTAGQDEVKAWTIQKNTTAKKAAGKIHSDIEKGFIRAEVCKYTDLKEFGSMNK
                     LKEKGLITLEGKEYIVEDGDIINFRFNV"
     gene            2033..2971
                     /locus_tag="PK733_18505"
     CDS             2033..2971
                     /locus_tag="PK733_18505"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12560.1"
                     /translation="MPKKPRQKHCEAIYHIMCHSIPEILLFRDNDDKDYYLRLIKRYT
                     DENQCSVYAYCLMDNHVHIHLDPRGYDISKFMRSLNTAYVSYYNKRHKRRGHLFQDRF
                     ESRILNTDQYNLAVSAYIHKNAKAIQGYLGKEEAYKYSSYGIYLGITRDEYKLVDMSF
                     IMGLFNITDYKIFTEKYFDFVSNKSDDSSIKELQKNLAAAPEYVYLSGRKIILRDKPP
                     SEIISHIYDRLFNLGQETKNVKESIAVNTNKAIYKCRAISAYALRVLCGLGYKEICNH
                     LYNITISGCSRLCSKGYELIIGNDIYKDIWNELVCY"
     gene            complement(3388..>3981)
                     /locus_tag="PK733_18510"
     CDS             complement(3388..>3981)
                     /locus_tag="PK733_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOJ12561.1"
                     /translation="ITHGVASLLVLWALPFVSIIAYKNGTAIDVIGVTIFCISIFMMF
                     LMSTFYHVMEPETKHKKVMRILDHIFIYVAIAGTYTPIAISVIGGWQAVVILIIQWVM
                     VLFGILYKSLSQLSMPKISLFIYLIMGWTLLIFMPLFINKANPALFELILGGGILYSA
                     GAIIYAKKGFKYHHMVWHLFVFLGALTHFIGICFFMY"
BASE COUNT         1408 a          584 c          769 g         1220 t
ORIGIN      
        1 cttttaatct gagcaaagga taagtaaaat tgttgtttga gtaaaattgt ttcttaattt
       61 tatttcaaac aattttatga gttcaatttt gcccggagct gaatttaatg gctctaagca
      121 gcgtaacgac ggctactggt aaatatttct tcacttttag taaatgcacc ctgcatccat
      181 ttcctatttg aaaaaaacag atattctgat acaataagaa atataaaacc attgaagtca
      241 ataacccatc actaaagtga ctggtttgta aaagccatta ttgactaaac taagcgaaag
      301 ctatgttaaa atagccatca tacccatgaa tgtttatctt agtttttggc tctatggctt
      361 accattaaac aatcctgagg tgtagggata gtgtggtaag catgacagac tattttaact
      421 ttgtagaagg ataacaactg gtttggtagt tccagaatag atgtgaaaac ttatctatta
      481 caaaaattac ctaacaatag aaaggaggat ggcgtttcct ctcatgactg aagtcacgag
      541 tctctacgcc taaatttatg aaaattggat taattggact gccatcggta gggaaaacaa
      601 ctttatttaa tttattaaca gggtctgaaa tggaggtttc ggatttttca aggtcaaaaa
      661 tagacgcaac tttggggatt gcaaagatac cagatgagag aatagacttc ctggcgaaaa
      721 tgtatgaccc taaaaaaatt acttatccca caattgaagt agtagatgta cccggtctgg
      781 taaggggatc cagtgcagga gaaggtgtag gcaatcaatt tcttgataac ataaggaaaa
      841 ctgatgccct tattcatatt ttaagagctt ttgaaagcga aagtgtagtc catgcagaag
      901 gaaatattga tcccatgagg gatatagaga cgattaatct ggagctttta tttgctgatc
      961 tgggtataat tgaaaacaga ataaaacgta tagaaacatc taaaaaggtt acaaaggaga
     1021 atctagaaga acttgaagtg cttaaaaaat gcagggaagg tctggaaaat gggcttctga
     1081 tacacaatct ggatcttagt gatgaggaaa aagaacacct taaaaccttc gcttttttat
     1141 ctgaaaaacc tatgatactg gttattaatg tagatgagaa tcagtttatg acgggcgact
     1201 atctttcaaa agaagcgcta ttagcttaca ctaaacaagt aaatacaccg attattgaat
     1261 tatgtattaa gtcggaactg gaaataagcc agttggagct tgaggataga aaaatgttca
     1321 tggaagattt gggtatttcc gaatcaggag tagataaact ggccaaaaca gcttatggtt
     1381 acctgggatt aatatctttc cttactgcag gtcaggatga ggtaaaagcg tggaccatac
     1441 agaaaaatac tactgcaaag aaagcagcag gcaagatcca ttccgatata gaaaaaggtt
     1501 ttataagggc ggaagtttgc aaatacacag atttaaagga atttggttca atgaacaagt
     1561 taaaggagaa aggacttatt accctagagg gaaaggaata tatagtagag gatggagata
     1621 tcattaactt cagatttaat gtgtaatgtg tgtaatgtgt gaagtatcag ggcagaattt
     1681 aaacactcac ctgagatgat agggacgctt ctcaaaaagc agatgatcat gagatgatag
     1741 ggacgcttct caaaaagtga gatgataggg acgcttctca aaaagtaaaa atgaataaga
     1801 ttaaaagtct tctcaggatg gatgggaaag gttatccaaa tgagaagact tttttattaa
     1861 tgaggtaatg tgaggtgatg gggacttttt cctaaaaaat gatggtaata aagggaagct
     1921 tcttaaaaaa atacaagttt tcacaatatc tctttacatg gctcatctta tatgttaaaa
     1981 tatgtaatgt aagataataa aacacataaa tgagtttaaa tgggggataa atatgcctaa
     2041 aaagcctaga cagaaacatt gtgaagcaat ttaccacata atgtgccata gtattccaga
     2101 gattcttctt tttcgggaca atgatgataa ggactattac cttaggttga tcaaaaggta
     2161 tacagatgaa aatcaatgca gtgtctatgc ctattgtctt atggataacc atgtacatat
     2221 ccaccttgat cctagaggat atgatatttc caaatttatg cgtagtctta acactgctta
     2281 tgtgagctat tataataaaa gacacaaaag acgtggtcat ttattccaag acaggtttga
     2341 aagcagaata cttaacactg accaatataa ccttgcagtt tcagcctaca tacataaaaa
     2401 tgcgaaagct attcagggct atttggggaa agaagaagct tataaatatt catcctatgg
     2461 catttattta ggaattacaa gggatgaata taaattggta gacatgagtt ttattatggg
     2521 actcttcaat atcactgatt ataagatttt tactgagaaa tattttgatt ttgtaagcaa
     2581 taaaagtgat gatagtagta ttaaagagct gcagaagaac ttggctgctg cgccagaata
     2641 tgtatatctt agtggtagaa agattatatt aagggataag ccaccttcag agataatatc
     2701 tcatatttat gatcgattat ttaacctagg acaagagact aagaacgtaa aggaatctat
     2761 tgctgttaat acaaataaag ctatttataa atgccgtgct atcagtgcat atgctcttag
     2821 agtgttatgc gggctaggtt ataaagaaat atgcaaccac ctatacaaca taactatatc
     2881 aggctgttca cgtctttgca gcaaagggta tgaactaatt ataggaaacg atatttacaa
     2941 agacatatgg aatgaactag tttgttattg aagtgctagg ttgaccaagt gtatgaatgt
     3001 atattagcgt gttaatgtat atatatgtat atgtttaaat atacttgcat ctaatggtgt
     3061 ttagatatag taagcgtatt taaaattacg tgttttatat taatggatta tatagtttcc
     3121 caggtaatta gaaaatcctt attctgtata ttttcatttc ctattctttt ttcaaggaac
     3181 atgtttctat tccatacttt atgttttata gttttgcaaa aataccattt ttagcaacac
     3241 aagcgtcctt ttttattatc tcaatatttt tttagagaag tagagaagcg ccccgtttta
     3301 cgttttagtg agaagcgtcc ccgttttacg cccgttttac gttgaagtga ggatgtcacg
     3361 ttttattact gttttattac tgtttgatta gtacatgaaa aaacaaatgc caataaaatg
     3421 cgtaagagca ccaagaaaaa caaataaatg ccataccata tgatgatatt taaatccttt
     3481 tttagcgtag attatggcac ctgcggaata tagtatacct ccccccaaaa taagctcaaa
     3541 aagagcaggg ttggctttgt ttatgaataa tggcatgaaa attaacagag tccatcccat
     3601 tatcaaatat atgaaaagtg atattttggg cattgaaagc tgggataatg atttatacaa
     3661 tattccgaat aataccatta cccattgtat aataagtatt actacagcct gccatcctcc
     3721 tataacagat atggcaattg gggtataagt acctgctatg gcaacatata taaatatatg
     3781 atcaagtatg cgcatcactt tcttatgttt ggtttccggc tccatgacat gataaaaagt
     3841 ggacataaga aacatcatga aaatagatat gcagaagatt gttactccta taacatctat
     3901 agcagtgcca tttttatatg ctataataga tacaaatgga agtgcccaca ataccaacaa
     3961 cgatgctaca ccatgagtta t
//
LOCUS       DAOSDD010000332         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8021_length_3969_cov_4.545478, whole genome shotgun sequence.
ACCESSION   DAOSDD010000332 DAOSDD010000000
VERSION     DAOSDD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8021_length_3969_cov_4.545478"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..234
                     /locus_tag="PK733_18515"
     CDS             76..234
                     /locus_tag="PK733_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12562.1"
                     /translation="MGKDKGILGNLFGNDTVLWFIILFLLIFWCCGYLGGTGYYREEK
                     NDIGLNLD"
     gene            947..2005
                     /locus_tag="PK733_18520"
     CDS             947..2005
                     /locus_tag="PK733_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ12563.1"
                     /translation="MEATDPLPEGHRYKIGLAYNLKKGIESDIEDIEAEFDSIETVNA
                     IKEALEMLNCSVELLEADASIITKLDQVKPDIVFNIAEGLHGRGREAHIPALLNFLHI
                     PFTGSDETTLCIALDKALTKRILTTFGVKTPKYQLIEHSSEFSKKLNFPLILKPNAEG
                     SSKGVSDFAIVYTTNELENLIDKSYEMYQQPILVEEYIKGREFTVGIIGNGNGTLVFA
                     PMEIKYRKKEEKYNIYSYSVKKNYKEYVEYICPPQIEKKIEEKLVRTAKKIYESLSCK
                     DFSRIDFRVSMDGEVFFLEINPLPGLAPGYSDFPMIGEFHGISYEELIKMVLNSALKR
                     YGMEPVSRGDRYAGLQNH"
     gene            1983..3089
                     /gene="ablA"
                     /locus_tag="PK733_18525"
     CDS             1983..3089
                     /gene="ablA"
                     /locus_tag="PK733_18525"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOJ12564.1"
                     /translation="MRDYKTIDIWKNIGTEEWNDWKWQIKNRIVSVEQLGKVMDISNK
                     EQQEIDICLERFRMAITPYYSTLIDPDDSNCPIRTQAIPSVKELIVQESDMADPLGEE
                     LYSPVENIIHRYPDRVLFLLTHKCSMYCRHCTRRRMVGEEDFSISEEILDKAVEYIRN
                     KTAVRDVLLSGGDPFVMTDARLEHIIQRLREIPHVEVIRIGTRTPVVLPMRITDNLVD
                     MLKKYHPIWINTHFNHPKEVTDIAVRACEKIIDAGIPLGNQSVLLKGVNDNAEILKEL
                     FLKLVKARVRPYYLYQCDLAQGIGHFRTKVEVGIEIMQNLRGYISGYAVPVFVIDAPN
                     GGGKIPIGPEYVISMEGGKVIMKNYKGDIYIYPN"
     gene            3831..>3969
                     /locus_tag="PK733_18530"
     CDS             3831..>3969
                     /locus_tag="PK733_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOJ12565.1"
                     /translation="MFRIVKKEILNSSVKLMEVEAPYIARKAQPGQFIILRVNEDGER
                     IP"
BASE COUNT         1342 a          585 c          833 g         1209 t
ORIGIN      
        1 cgtaacaaaa ctaaagtact ctgaataata tgatatagaa gatattttca tattttttaa
       61 ggaggtattg tttaagtggg aaaagataag ggaatattag gaaacctttt tgggaatgac
      121 acagtattat ggttcataat tttgtttttg cttatattct ggtgctgtgg ttatttgggt
      181 ggaacaggct attaccgtga agaaaaaaat gatattggct taaatctgga ttaaagtcta
      241 atacttcgtc ctctattaga gtttaaagct tcaaattgaa gcataccgga tttatagggc
      301 ctgtacatag caatttagtt atttataaat gtatttttat gatatagcag gccctacatg
      361 cagagaacag ggacactctg caacaaaaat acctgcacag cagcggtgtg tccccgttcg
      421 ttattcaaat tgtgagcgaa ataagttttc taaagggaca agccttcaga ggaaatttgc
      481 gggaattgct tcgtaatctg cattattacc gatggttgaa cttggaatgt attgatgctg
      541 aattagtttt tgcgttgaaa taaagttttg tccataagct gtaacaggtt gaatagattg
      601 taatataaac ttaaagaata tttagcattt gtagtttaca taaatcatta aaattaacta
      661 atacggcaat aaatttcaat ataaaataat atggaagcat aaactgttaa atttgtttaa
      721 actatgtata tatgacgtaa aataatgaat ttcaaccatt tattttatat atgtttttgc
      781 tataattgta gagaagtatt tgagctcaat tctatgtaaa tccaaccccc gggcacatgg
      841 tatattcaat ggatcagagt attttctacc attttccggc tgttgacaat tggagaattg
      901 aactctaatt gctaaaacct tatatcagga gatgattaat ttggagatgg aagcaactga
      961 tcctttacct gaaggtcacc gttataaaat agggttggcc tataatttga aaaaaggcat
     1021 tgaatccgat attgaagaca ttgaagctga atttgacagt attgagacgg taaatgcaat
     1081 aaaagaagct ttggagatgc taaattgcag tgttgaactt ttagaagcag atgcaagtat
     1141 tataactaaa ttagaccagg taaaaccgga tattgttttt aatattgcgg aagggttgca
     1201 tggaagaggc agagaggcac acatacctgc ccttttaaat ttcttgcata ttccctttac
     1261 aggttctgat gaaactactt tatgcattgc tcttgacaag gcattaacaa agagaattct
     1321 aaccaccttc ggggtaaaaa ccccaaaata tcagcttatt gagcactcat cagaatttag
     1381 caaaaaattg aattttcctc taatattaaa gccaaatgca gaaggctcca gtaaaggagt
     1441 ttcagatttt gcaatagttt acactacaaa tgaattagaa aacttgattg ataagagcta
     1501 tgaaatgtat caacagccta tactggttga agaatatata aagggcaggg agtttactgt
     1561 cggcattatt ggcaatggaa acggtaccct ggtttttgct ccaatggaaa taaagtatag
     1621 gaaaaaagag gaaaagtata atatatatag ttacagtgtt aagaaaaatt ataaggaata
     1681 tgttgaatat atttgtccgc cacagataga aaagaagatt gaagaaaagc tggtcaggac
     1741 agctaaaaaa atatatgaat ccctatcctg caaagatttt tccaggattg attttagagt
     1801 atccatggat ggagaagtgt tttttcttga aattaacccg ttaccgggtc ttgcaccagg
     1861 ttacagcgac tttccaatga taggtgaatt tcacggaatt agttatgaag aacttattaa
     1921 aatggtattg aacagtgctc ttaaaaggta tggcatggag cctgtaagca ggggggatag
     1981 atatgcggga ctacaaaacc attgacatat ggaagaacat cggtactgaa gaatggaatg
     2041 attggaaatg gcagataaag aacaggatag tatctgttga gcaattaggt aaggttatgg
     2101 atatttccaa taaggaacag caggaaattg atatttgctt agagaggttc aggatggcta
     2161 taacccctta ttattctact cttattgatc ccgatgacag taattgtcct ataagaaccc
     2221 aagcaatccc atcggtaaaa gagttgattg tacaggaaag tgatatggct gatcctcttg
     2281 gtgaagaact gtattctcct gtagaaaata ttatccacag atatccagat agagttttat
     2341 ttcttcttac ccataaatgt tcaatgtatt gcaggcattg tacaagaaga agaatggtcg
     2401 gtgaagagga tttttcaatt agtgaagaaa ttttggataa ggctgttgaa tacatcagga
     2461 ataaaactgc tgtaagagat gtacttttat ccggagggga cccttttgtt atgacagatg
     2521 cacggcttga acatataata cagcggttaa gagaaatacc ccatgtcgag gtaatcagga
     2581 taggtacacg tacgcctgtt gtattaccga tgagaattac cgacaacctt gttgatatgc
     2641 tgaaaaagta ccatccaata tggataaata cccactttaa tcatccgaag gaagttacgg
     2701 atattgcagt cagggcatgt gaaaaaatta ttgacgcagg cattcctctt gggaaccaga
     2761 gtgttcttct gaagggtgtt aatgataatg ctgaaattct gaaggagtta ttcctgaagc
     2821 tggtaaaagc aagagtaaga ccttattatc tgtatcaatg tgatttagct caaggaatag
     2881 gacacttcag gacaaaagtt gaagtaggca ttgaaataat gcaaaatctc agaggatata
     2941 tttccggtta tgctgtgcct gtttttgtta ttgatgcacc aaacggcggg ggtaaaattc
     3001 caataggccc ggaatatgta atatctatgg aaggtggcaa agtaataatg aaaaattaca
     3061 agggtgatat atatatctat cctaattaga ggtgggaggt gagaagtggg aagctagaag
     3121 tgagatgttg gaagctggat gtgagatgct ggaagttgga agttggaagc cagtgggcag
     3181 agactaagta gtgaataatt tccagttgca atgtttgcag caataaaaaa tattttgaaa
     3241 gcacaatgat ttaaagcttt agaaaatgct acctaactta aaccggaaat ggcctcttac
     3301 ttggatgtga gaagtcagta tctcttttac tacctctcac ctctcttatc ctgcttctta
     3361 tttctgactt cttaccttta acctctcacc tccaacctct aactttgttc aaatcatata
     3421 cttgattcta ctgcaataac tcagattttg gtgcaaaagg agaatgaagc ttttgaaaca
     3481 cgttgcagaa tttatcacta atatttaaac tttttaaggg ctgaaacgcc acggatggcg
     3541 tgaaaaacac tgcttaagac agaatgtcga atcagtgcgt gcccttcaga gtgcaagatg
     3601 aagtgttgca aatttcaacg tgttagcact gaatgagttt ttgtaccaaa atactatttt
     3661 gttaatcgga taatgtcata ttttttatca tacacagaaa atgtgcctct ttttatgctc
     3721 cttttgttga cgaaacaagt tattaaatat aaaattagta tctggggaag catggaaaca
     3781 caaggcagtt tttctgcttc tacccaagaa tgaaaggggt aacttacttt atgtttagga
     3841 tagtaaaaaa agaaattcta aattcctcag ttaaactaat ggaggtggaa gccccgtata
     3901 tagcaaggaa agcgcagccg gggcaattta ttattttgag ggttaatgaa gatggtgaaa
     3961 gaattccat
//
LOCUS       DAOSDD010000333         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8050_length_3959_cov_6.023566, whole genome shotgun sequence.
ACCESSION   DAOSDD010000333 DAOSDD010000000
VERSION     DAOSDD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8050_length_3959_cov_6.023566"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK733_18535"
     CDS             <1..543
                     /locus_tag="PK733_18535"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ12566.1"
                     /translation="SIDRGSPKGKRDYAILLTVTRLGLRVSDIRSLEFSSLNWPRRTI
                     NLNVQKTKKAIELPLLDDIGWAIIDYLKNGRPKTKCTRVFVRHRAPYDAVGENESFYK
                     ELHRYMKSAGITPPSGIHCGLHSLRSTLARRMLETGAPLPVISETLGHANINTTSIYL
                     KIDLEGLKKCPLDPEEVFSL"
     gene            540..1517
                     /locus_tag="PK733_18540"
     CDS             540..1517
                     /locus_tag="PK733_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12567.1"
                     /translation="MNNGYVWKSAIAQKAQQFLQIKHMTGFKYDAQEKYLQRFDSYYF
                     QNGYTGIRITKEMTDGFIYCPDDRLSGWYVKERLLRDFAVFLKDQCFSEIYIPIVQSA
                     PPRSSFTPYIFTDNEIRRLFEAVDSWEDSPLTNRVLIDPVFFRLLYSTGMRLSEALNL
                     MVKDFDNSVLTVYHAKNNKDRLVPLHPNMAEKLKNYTENMHRFSKESDYLFPSSRGYD
                     CCIDQSTIHRRFRQYLSRAEISLTGTGPRIHCLRHNYAVKCLKKWVLNGNELTNMLPY
                     LAAFMGHSDFRETQYYIRLTADLYPDIISRIEAEFGYVIPEGGSNNEGV"
     gene            1504..2526
                     /locus_tag="PK733_18545"
     CDS             1504..2526
                     /locus_tag="PK733_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ12568.1"
                     /translation="MKGYDFPKYLSSYLSKYLPGQKNASKNTITSYCDTFKLFLIFCE
                     EEKKMKPEKICISDISKELVVEYLSWLEAKRRCSISTRNQRLAVIHSFFHYVQKESPE
                     NLHEIQKILSIPVKKHPKTMVPYLTGNETQILLAQPDPSSYEGYRDMVLLSVLYDTGS
                     RVQELVDIKTKDVRLTAPAVITLHGKGSKARTVPIIGKTANLLEMYLDRKKYHAGIAK
                     GDNYLFVNQKQQHLSRWGISYILDKYVEMAKGNPEFQVSFPITPHVLRHSKSMHLLQS
                     GVNLIYIRDFLGHVDCSTTEVYVRADSEMKRKAIENAYVDLVPDEIPRWEEDGELMQW
                     LSNFCK"
     gene            2699..2914
                     /locus_tag="PK733_18550"
     CDS             2699..2914
                     /locus_tag="PK733_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12569.1"
                     /translation="MIFECINKAGKRKEKWPGRIIYIKNYGSHFELFIQSHSSIFVLF
                     GKTSCGFFASMPDFNAGCHLVNPRDIF"
     gene            3464..>3959
                     /locus_tag="PK733_18555"
     CDS             3464..>3959
                     /locus_tag="PK733_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12570.1"
                     /translation="MFNIPSARLTVPRAVAGEIIRKNLIDTVLNSPTKLVYIHAGAGH
                     GKTTLLSQVSNSAENVVWLSLDGENDIFTFINTLCEAVKRTFPEFDFSLPEYLPFSEK
                     NNFISMLAGALICSIENIADDFIMVMDDFHTIEEDEVKKLIACLIKYLPNNAKLCFGS
                     REAPP"
BASE COUNT         1200 a          808 c          802 g         1149 t
ORIGIN      
        1 tccattgata ggggaagccc aaaaggtaag cgagattatg ctattttgct tacagtcacc
       61 aggctcgggt tgcgagtgag cgatatccgt tctcttgagt tttccagcct aaactggccg
      121 cggaggacta tcaacctaaa cgttcaaaaa actaaaaagg caatagaact cccccttctt
      181 gatgatatcg gatgggcaat catagattac cttaaaaacg ggagaccaaa aacaaaatgt
      241 acacgggttt ttgtaaggca tcgggctcct tacgatgctg tgggagaaaa tgaaagcttc
      301 tataaggagt tacaccgcta tatgaagtct gccggaatca ctcctccgtc agggatacat
      361 tgcggactcc attccctcag aagcactctc gcaaggagaa tgcttgaaac tggcgcaccg
      421 cttcctgtga tttcagaaac acttgggcat gccaatatta ataccaccag catttactta
      481 aaaattgatc tggaaggtct caaaaagtgc ccactcgacc ctgaggaggt gttctcatta
      541 tgaacaatgg atacgtatgg aaaagtgcta ttgcacagaa agcacagcag tttcttcaga
      601 taaagcatat gacaggattc aaatatgatg cacaggagaa atatcttcaa agatttgatt
      661 catactactt tcaaaatggt tatactggaa tacgtatcac taaagaaatg actgacggct
      721 ttatttactg cccggacgat aggctatccg gatggtatgt aaaagagcgg cttcttcggg
      781 attttgcagt atttctgaaa gatcagtgct tttctgagat ttatattccc attgtacaat
      841 ccgctcctcc aaggagttca tttactcctt atatatttac agataacgag ataagacgtc
      901 tatttgaggc agtagactca tgggaagatt cccctcttac caaccgtgtc ttgattgatc
      961 ctgtcttttt ccgactgctt tatagtacag gcatgcggtt atcagaggca ctcaatctca
     1021 tggtgaagga ttttgataat tctgtcctta ctgtatacca tgcgaagaat aataaggatc
     1081 gtcttgtacc gctgcatccg aacatggctg aaaaacttaa gaactacacc gaaaatatgc
     1141 accggttttc aaaggaatca gattatcttt tcccatcttc aagaggatat gactgctgta
     1201 ttgatcagag caccatacac cgtagattta ggcagtacct ttcccgtgca gaaatttccc
     1261 ttacagggac tgggcccaga atccattgtc tcagacacaa ttatgccgtc aaatgcttaa
     1321 agaagtgggt gcttaatgga aatgagctga caaatatgct tccttatctt gctgcattca
     1381 tgggacattc agattttagg gaaacacagt actacatccg cttaacggct gacctgtatc
     1441 ccgacatcat cagccgtata gaagcggagt ttggatatgt gattccggaa ggaggcagta
     1501 ataatgaagg ggtatgattt cccgaaatat ctttcctctt atctgtcaaa atatcttccg
     1561 gggcagaaaa atgccagtaa gaataccata acatcctact gtgacacttt caagcttttc
     1621 ctgattttct gtgaggaaga gaaaaaaatg aagccagaga aaatctgtat atctgatatt
     1681 tcaaaagaac tcgttgtgga atatcttagc tggctggaag ctaaaaggag atgttcaata
     1741 tccacccgca accagcggct tgcagttatt cattccttct tccattatgt tcagaaagaa
     1801 tcaccggaaa atctgcatga aatccagaaa attctgtcca taccggttaa gaagcaccca
     1861 aaaacaatgg ttccttacct gactggcaat gaaacacaga tactacttgc tcagccagat
     1921 ccgtccagtt atgaagggta tcgtgacatg gtactgttgt cagttcttta tgatactggt
     1981 tccagggtgc aggaactggt agatataaaa acaaaggatg tccgtctgac cgctccggca
     2041 gttattacac ttcatgggaa aggaagcaag gcacgcactg taccaattat tggtaaaacg
     2101 gccaatctgt tggagatgta tttagatagg aagaaatacc atgctggaat agccaaaggc
     2161 gacaattacc tgtttgttaa tcagaaacaa cagcatctct cacgttgggg gatttcctat
     2221 atactggata aatatgtaga aatggcaaag ggcaatcctg aatttcaagt tagctttcca
     2281 attacgcctc atgtgcttcg ccactcaaaa agtatgcact tgcttcagtc tggtgtaaac
     2341 ttgatttata ttcgtgattt ccttggacat gtggattgtt ctacgaccga agtgtacgtg
     2401 agagcggaca gtgaaatgaa acgcaaagcc attgagaatg catatgtaga tttagttcca
     2461 gatgaaattc cacgttggga agaagatgga gaactgatgc aatggttaag taacttctgc
     2521 aagtaaaata tcatctatag tattatgggg agtgattttg ctgaaaatag cataaaatac
     2581 gaggaaattg cattcactcc ccataatatt tgactccaca taatggtgaa gatcatgaaa
     2641 acgaatatgt ggcaattcaa agcgcctgag caactgtccg ataataggag gtttataaat
     2701 gattttcgaa tgtataaata aagcaggtaa acgtaaagaa aaatggccgg gtaggattat
     2761 ctacataaaa aattatggca gtcattttga attattcatc caaagccact caagtatatt
     2821 tgtactattc gggaaaacct cctgtggatt ttttgcaagt atgcctgatt ttaatgcagg
     2881 gtgccatctt gttaatcctc gtgatatttt ttaaaatact gaaagtcttt caagggttct
     2941 gggaaaggtg ttaagaaaat cctataatag attctattcc ccctaatttc ttttaaatat
     3001 ttgctcattc tgtcactctt ttgccgtaac cacatatact acattcaaaa atcgtcactg
     3061 tcttcaggct tgctccatat ggaagagaag ccgatatgtc acaatacggg caaatctttt
     3121 catatgaaac catgaatgag gcgtcgtttt tgcggcagca cttccccatg cagggaaaca
     3181 tttcctcatc cacgccgcag tcttcatcta gattttttta tcatatcagt attcatctaa
     3241 atcacctcgc tgtcttttac gaccaatatg tatatcaaca gagcatattg cttttagata
     3301 attataaacc atcggcaatt aacaaaaccg tgaacataac tgcgattttt cacctgtttt
     3361 ccttttctga ctttttattg cttttttcat taaaaattgt tgcgaattat ctgattttat
     3421 gttaaaatat agtaatttag tattagttgt ggagtgtatg tgtatgttca atataccgag
     3481 cgcgaggctg acggttcccc gagccgtcgc aggagaaatc atacgcaaaa atttaattga
     3541 taccgtcctt aacagtccca caaagcttgt gtatatccat gccggcgcgg gtcacggcaa
     3601 gacgaccctt ctgtcgcaag tttcaaatag cgccgaaaac gtcgtctggc tttctctgga
     3661 tggagaaaac gatattttta ccttcataaa tacgctctgc gaggctgtaa agcggacctt
     3721 tccggaattt gatttttcct tacctgaata cctgcccttt tccgaaaaaa acaatttcat
     3781 ctccatgctt gcaggtgcac tgatctgcag cattgaaaat attgccgatg attttattat
     3841 ggtcatggac gattttcaca ccatagaaga agatgaggtc aaaaaactta tcgcctgctt
     3901 gatcaagtat ctccctaaca atgccaagct ttgtttcggc agcagagaag ctcccccac
//
LOCUS       DAOSDD010000334         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8054_length_3957_cov_5.783957, whole genome shotgun sequence.
ACCESSION   DAOSDD010000334 DAOSDD010000000
VERSION     DAOSDD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_3957_cov_5.783957"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..>3957)
                     /locus_tag="PK733_18560"
     CDS             complement(374..>3957)
                     /locus_tag="PK733_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ12571.1"
                     /translation="AENMFHIVWYRPIIYDEYRISYKVKKASAKISDYVPIEEDVLRS
                     GLPPLWGYSTMLEKPKGIMKDVDPDIQGDEVFEITSKVLLGDHNTIIDSTMTISNYEG
                     ELPEGYSADADLPVGNLSININDDGNGGEAFDISFIIPLDVILSEVPIEQQGLKSANA
                     VSSVASIIGTRVPHQLVRIFGNKTVIEAGLDSYSLVDTVTGGYAATKHIKMLEFLNKQ
                     VHTPCYNESNKYYDGIAIENEMRDTQTRAFGKAALNIAFMALGFIPGIGWGAALAIAV
                     GTTIVGYVVDKALDNYQDKKYEELKARVESNMKPGCKLPDGSDDKGDDSVPIFRPMVV
                     VACDPSGFIYEGVESNRIKGVTATALEKVNGEWEVWDAGWFGQENPLITDNNGFYQWD
                     VPEGAWKVMYEKEGYEVAFSEELPVPPPQLEVNVGIVSLAAPEVEKIKAEPGGIIDLV
                     FDRYMKVSIVNDSTFKVYEKTLGDNGNKVVIAGSITAINAEPDPKDANILLAKNFKFI
                     PVTPLNTSDECSVWVSHMVENYADRTMNVDFLDCVIIPEKVIQVESVSLNKSSLTMYP
                     GDGSQLLATVMPQNSNDTSVSWSSSNPGVAGVNNSGIVTAYAVGTATITVTTTDGGFT
                     DTCLINVVSRPVIIPDPDPDPGAGGSTGTTPVKKPVIGVKLNKNVLELIVGDSSAIGD
                     SIGDGSVMVVMVGDGSALLAAEIIPSDATNKKVTWASSDETVAKVDNGKVSPLKEGSA
                     IITVTTEDGNYKDTCTVIVIAKPERDPNKYSSTVGVNASEVSAFDDEISLAFETGIFY
                     SSTPIEITKLLQKDAELLFKKDKSRLKLISGSPVYNISLGGITPNRPVEIVIKYEEIL
                     LDKIDPLKLGIYKWDENKSGWVWAGGAVDLHNGLVQGTISSEGKYTVMAYDKSFTDID
                     GHWGNKDIEILASRHVINGINDNEYAPDRNITRAEFVKLIMSMVPSCPVVEAKRTQQS
                     ETYTDVSKEKWYFPYIEAATEFGIVKGNNGAFNPDSLITREEMAVMIIRILNSSNELN
                     KIVDEYSGEQSLNFKDAGEISQWALGAMILANQKGIIRGDANNSVKPKASATRAEAAA
                     VILRTMEILGFLKAPVTIIGILKEVNIEGTHYELEYSCDCAAGKINYVAIPLDSIIEG
                     HMRLFLGKEVELTGILQKGGSIYMRGPVLKVISIWDRIRAKCTTCEN"
BASE COUNT         1102 a          885 c          628 g         1342 t
ORIGIN      
        1 ttcacgacac ggatgtcgtg agccgtcacc aaaaccatgg atggtgaatg tgacgagtag
       61 aatttcaagg catcgaatct atctttgtgt cagaaacgaa cgatgaacag aataaaatat
      121 ttgaccttct taacactaaa ttggtatagc catacggttg aaaataaaaa agatgctaaa
      181 acccgcaata tgacttgacg ggtttccttt ttttctcatt ggaagttacg tcttagtcaa
      241 tagtttgaaa gaaatgaaaa tttctgtaaa actaatgcgt atgtacatat ttttgaggct
      301 gtcaaacaga gtgtgtaaat aaacgctctg tttgacagcc tccagcctcg acagttttta
      361 tattggatta ttgctaattt tcacaagtag tgcatttggc tcttattctg tcccatatag
      421 aaattacttt caatacaggt cctctcatat atatgcttcc gcctttctgc agtatacctg
      481 ttaattctac ttccttaccc aggaataatc tcatatgtcc ttcaattatg ctgtccagag
      541 gtatggcaac ataatttatt tttccggcag cacagtcaca tgaatactcc aattcgtaat
      601 gggtgccttc aatatttacc tctttgagaa tacctataat ggttacaggt gctttcaaga
      661 aacctagaat ttccatggta cgaagtatta ctgctgctgc ttccgctctt gttgctgaag
      721 cctttggctt aacactgtta tttgcatcgc ctcttatgat ccccttctga ttcgcaagta
      781 tcatggctcc aagcgcccat tgggaaatct caccagcatc tttgaaattc agggattgtt
      841 ctcctgagta ttcatcaaca attttgttta attcattaga actgtttaga attcttatta
      901 tcataacagc catttcttct ctggttatta aactatcagg attgaaagct ccattatttc
      961 cttttactat gccaaattct gttgctgctt ctatatatgg gaaataccat ttctccttgc
     1021 taacatccgt atatgtttca ctttgttggg ttctctttgc ttcaacaaca ggacatgacg
     1081 gcaccatact cattatgagc ttcacaaatt cagctcttgt aatattcctg tcaggtgcat
     1141 actcattatc gttaattccg ttaattacat ggcggcttgc aagaatttct atgtctttat
     1201 tgccccagtg tccatctata tctgtgaatg atttatcata tgccatgaca gtatattttc
     1261 cttcagagct tatagttccc tgtacaagac cattatgaag gtcaacagcg cctcctgccc
     1321 aaacccatcc ggatttattt tcatcccatt tgtaaattcc cagtttcaaa ggatcaattt
     1381 tatctaatag gatctcttca tattttatca ctatttctac aggacgattt ggagttattc
     1441 ctcctaaaga tatattatat acaggtgaac cagaaataag ttttaatctt gacttatctt
     1501 ttttgaatag caattctgca tctttctgta ataattttgt tatttcaata ggtgtagatg
     1561 aatagaatat acccgtttca aaggcaagtg atatttcatc atcaaaagca gaaacctcac
     1621 tggcgtttac tcctactgta ctgctgtatt tgtttggatc tctttccggt tttgcaatta
     1681 caataacagt acaagtgtcc ttataattac catcttctgt tgtaacagta ataattgctg
     1741 atccttcttt taatggagat acctttccat tgtccacctt cgctacagtt tcatcacttg
     1801 aagcccaggt gacctttttg ttggttgcat cggatgggat gatttctgcg gcgagaagtg
     1861 cagaaccgtc cccaaccatt acaaccatta cagaaccgtc cccaattgaa tccccaattg
     1921 cagaactatc acccactatt agttcaagta catttttatt aagctttaca ccaataacag
     1981 gctttttaac aggagttgtt cccgtactgc cgcctgcacc cgggtcagga tccggatcgg
     2041 gaatgataac aggtcttgag acaacgttta ttaagcatgt atcggtaaat cctccgtcag
     2101 tagtggttac tgttattgta gctgtaccaa ctgcataggc tgtaacaatg cctgaattat
     2161 taacaccagc tacgccagga ttacttgaac tccatgatac acttgtatca tttgaatttt
     2221 gcggcatgac tgtagctaaa agttgagaac cgtccccggg atacatggta agtgatgatt
     2281 tattgaggct cacactttct acctgtataa ctttttcagg aattattaca caatccaaaa
     2341 aatcaacatt cattgtcctg tctgcataat tttctaccat atgacttacc catacactgc
     2401 attcatcact tgtatttaat ggtgtaacag gtatgaactt aaagtttttt gccagcaata
     2461 tatttgcatc cttcggatca ggttcagcat ttatggctgt tatacttcct gctattacca
     2521 ctttatttcc gttatcacca agtgtttttt catacacctt gaatgtacta tcattaacta
     2581 tgctgacttt catatacctg tcaaaaacaa ggtcaattat tcctccaggt tctgctttaa
     2641 ttttttcaac ctccggtgcc gccagtgaaa caatacctac attcacttca agctgaggtg
     2701 gcggtacagg aagctcctca ctgaaggcta cttcatagcc ttccttttca tacataacct
     2761 tccatgcacc ttccggtaca tcccactggt aaaatccatt attatcagta attaacggat
     2821 tttcctggcc aaaccaccct gcatcccaga cttcccattc accgttaact ttttcaagag
     2881 ctgtagcagt aacacctttt atgcgattac tttccacacc ttcataaata aaaccacttg
     2941 gatcacatgc taccacaacc attggtctaa atataggtac tgagtcatcg cctttatcat
     3001 cgctgccatc aggcaattta caccctggct tcatattact ttctactctg gcttttaatt
     3061 cttcatattt tttatcttgg taattatcaa gagctttatc aacaacataa cctactattg
     3121 tggttccaac tgctatagcc agggcagctc cccatcctat tccgggtata aatcccagtg
     3181 ccataaaggc aatattaagc gctgcttttc caaatgccct ggtttgagta tctctcatct
     3241 cgttttcaat agcaattcca tcgtaatatt tattactctc attatagcat ggagtatgaa
     3301 cttgtttgtt caaaaattcc agcattttta tatgcttcgt agccgcatag ccacctgtta
     3361 ctgtgtctac caagctgtag ctatctagac ctgcttcaat tacagtctta tttccaaata
     3421 ttcttacaag ctggtgtggt accctggttc ctataattga agctacagag ctcactgcat
     3481 ttgctgactt aagaccttgt tgttcaatag gaacttcact taagatgaca tctagtggaa
     3541 taataaaaga aatatcaaat gcttctcctc cgttaccatc atcattaata ttaatactaa
     3601 gattacctac aggtaaatct gcatctgctg aatatccttc gggcagctca ccctcataat
     3661 tcgagatagt catagtagaa tctattatag tattatgatc cccaagtaat acttttgacg
     3721 taatttcaaa aacttcatct ccctggatat cagggtcaac atctttcatt ataccttttg
     3781 gtttttccag catagtagag taaccccaca aaggaggcaa tccacttctt agaacatcct
     3841 cctctatagg cacataatct gaaattttcg cagatgcctt cttcacctta tatgagatac
     3901 ggtattcatc atatatgata ggacggtacc atacaatatg gaacatattc tcggctt
//
LOCUS       DAOSDD010000335         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8095_length_3941_cov_3.955739, whole genome shotgun sequence.
ACCESSION   DAOSDD010000335 DAOSDD010000000
VERSION     DAOSDD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8095_length_3941_cov_3.955739"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1045
                     /locus_tag="PK733_18565"
     CDS             65..1045
                     /locus_tag="PK733_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOJ12572.1"
                     /translation="MEIGKVPNDILKEIVIDKIKHNRKEVILRPQIGEDCCGVDFGEY
                     ICVLSSDPITGAVNEIGKLAVHISCNDIASCGVEPLGLLTTILAPDGTTRDDLNIVMT
                     QLTDTANSLNVDIMGGHTEITAAVNKLVIITTAVGKVLKGRLVTSSGAKEGDSIILTK
                     TAGMEGTAIIAHDREDELAGILGKEFIDKAKSFIDNISTVSEGVIAGKYGANAMHDVT
                     EGGVLGAVWEVAEASDKGVVIYKDRIPIAVETQKICEFFSLNPLRLISSGCMLISCSD
                     GNGLVKELENNGIKATIIGEIVKGNDKLICEGTREFKIDPPGSDELFKLK"
     gene            1169..2407
                     /gene="glyA"
                     /locus_tag="PK733_18570"
     CDS             1169..2407
                     /gene="glyA"
                     /locus_tag="PK733_18570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOJ12573.1"
                     /translation="MIKMDEVLKVDPEVAAAIENEINRQRNKIELIASENFVSQAVLQ
                     ALGSPLTNKYAEGYPGKRYYGGCEHVDVAENLAIERVKKLFGADHANVQPHSGAQANM
                     AAFFAVLNPGDTILGMDLAHGGHLSHGMAKNISGKYFNVASYGVRRDNYRIDYDDVLK
                     KAKEAQPKLIIAGASAYPREIDFKVFREIADEVNAYLMVDMAHIAGLVAAGIHSNPVP
                     YAHIVTSTTHKTLRGPRGGLILSNADLAKKIDSAIFPGIQGGPLMHVIAAKAVSFKEA
                     LSDDFKDYQNQIVKNAKALAASLMNKGFKLVSDGTDNHLMLVDLTNKGITGKDAQHML
                     DEVGITANKNGIPFDTQSPFVTSGIRIGTPAVTTRGMKEEDMEEIAELINLTLTDFDN
                     SRDRIIQNVKGICSKYPLYV"
     gene            2664..>3941
                     /locus_tag="PK733_18575"
     CDS             2664..>3941
                     /locus_tag="PK733_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOJ12574.1"
                     /translation="MFDKREPLAYRMCPRTIDEFAGQEDIIGKGKLLYRMIKADRIRS
                     IILYGPPGTGKTSLAKVIAATTKSQFEKLNAVTAGVADIKKIVADTHNQFLNPKGKTV
                     LFIDEIHRFNKAQQDALLPFVEDGTIVLIGATTENPFFEVNKALISRSSVFMLKPLME
                     KDIEKIILNALQDRERGLGEYDLEVDNEAVIYLASVSNGDARVALNTIELAVMTSDMD
                     KDGKIHININTIKECFQKRPLAFDKSGDSHYDNISAFIKSMRGSDPDAAVFYLARAIY
                     GGEDPEFLARRIIIAASEDVGMANPTALQVAVAAAEAVKMIGMPEAKIILSHAAVMVA
                     TSPKSNAAYIAISKAMDDIENKRTGEVPMHLRNPVTKGMKELGYGEGYKYAHDFKGNI
                     VDQEYMPEEMSGTIYYNPTTNGYEARIKEWLDRR"
BASE COUNT         1366 a          581 c          874 g         1120 t
ORIGIN      
        1 aaagttttgt ttctgcaagg gtgaattcat ttcgcacgca gcaggaatgg agcttaagat
       61 gaagttggaa ataggaaagg ttccaaatga tattttaaaa gaaatagtta tagataaaat
      121 taaacataac cgtaaagaag ttatactaag accccaaata ggtgaagact gttgcggggt
      181 ggactttggc gagtatatat gcgttctgtc aagtgatcct ataactgggg cagtaaatga
      241 aataggcaag cttgcagtgc atatatcatg caatgatata gcctcctgtg gagtagaacc
      301 tcttggactt ctgactacaa ttttagcccc ggatggcact acccgggatg atttgaatat
      361 tgttatgaca cagttgacag ataccgccaa ttctttaaat gttgacataa tgggtggaca
      421 tacggaaata acagcagcag ttaacaagct tgtaataatt acaacagctg tagggaaagt
      481 tctaaagggt aggcttgtca cttcatcagg cgctaaagaa ggggatagta taatacttac
      541 caaaacagca ggaatggaag gtaccgcaat aatagctcat gatagagaag atgagttggc
      601 tggtatattg ggcaaagaat ttattgacaa ggcaaaatct tttatagata atataagcac
      661 tgtaagtgaa ggggttattg ccggtaaata tggtgccaat gctatgcatg atgttaccga
      721 gggcggagta cttggtgctg tatgggaagt tgctgaagct tctgacaaag gtgtggtgat
      781 ttataaagat agaataccta tagctgttga aacacaaaaa atatgtgagt ttttttcctt
      841 aaatccgctc agattgattt ccagtggctg catgttaata tcatgcagtg atgggaatgg
      901 gctggtgaag gaactggaaa ataacggtat aaaggcaaca ataattggag aaatagtaaa
      961 aggtaatgac aaattaattt gtgaaggcac ccgagagttt aaaatcgatc caccgggatc
     1021 ggatgaattg tttaaattga aataaaaatt aatgtgaaat cctagtaatt gcagtaaatt
     1081 aaagctattg ccaagttatt gcagtgagat taaaataaaa taatgtatgt acaaacaaat
     1141 aaagaagaaa aagaaggagt tgtaaattat gataaaaatg gatgaagtat taaaagtaga
     1201 tccggaagtt gctgctgcta tagaaaatga aattaatagg caaagaaata aaatagagtt
     1261 aatagcttcc gaaaattttg taagccaagc tgttttgcaa gcactaggca gccctcttac
     1321 caataaatat gctgaaggat atcctggcaa gcgttattat ggcggttgtg agcatgttga
     1381 tgtggctgag aatcttgcta tagaaagagt aaagaaatta tttggtgcgg atcatgccaa
     1441 tgtacaacct cattcaggtg cacaggcaaa tatggctgcg ttttttgcag ttctgaatcc
     1501 tggagataca atacttggta tggatttagc tcatggcgga catttaagtc atggaatggc
     1561 aaagaatata tccggtaaat attttaatgt agcttcatat ggagtcagaa gggataacta
     1621 tcgtatagat tatgatgatg tcctgaaaaa agcaaaagaa gcgcagccta aattaattat
     1681 tgcaggagcc agtgcgtatc ccagagaaat agattttaaa gtttttagag aaatagcaga
     1741 tgaagtaaat gcttacctta tggtagatat ggctcatatt gccggactgg tagcagctgg
     1801 tattcattcc aatccagtac catatgcaca tattgttacc tcaacaactc ataaaactct
     1861 tcgtggtcca agaggaggct tgatactatc aaatgccgac ctggcgaaaa aaattgacag
     1921 tgctatattc ccaggtatac agggcggtcc acttatgcat gttattgctg cgaaagctgt
     1981 aagttttaaa gaagcacttt cagatgattt taaagactat cagaatcaga tagttaaaaa
     2041 tgccaaggca cttgctgcaa gtcttatgaa taaaggattt aagttggtat ctgatggcac
     2101 tgataaccac ctgatgcttg tagatctgac taataagggt ataactggaa aagatgctca
     2161 acatatgctg gacgaagtag gtataacagc taataaaaat ggtattcctt tcgatactca
     2221 gagtccattc gtgacaagcg gtattaggat aggaacaccg gctgtaacta cccgcggcat
     2281 gaaagaggaa gacatggagg aaattgcaga gttgattaac ttgactctta ctgattttga
     2341 taattccaga gatagaatta ttcagaatgt taaagggatt tgctctaaat atcctctata
     2401 tgtttaaagg taagaaattg tagaaacagt taaggccaga aaattaaaga tagaaaatta
     2461 aagataagaa aatagagatg gatagttgaa ggtaggaagc agaaaattga tagcaaaatt
     2521 ttcttttctt cctctaaacc ttcctttaac tttgactcta atctaatctt ccatattcta
     2581 aactttaaat tctatctgga aattttatct agaaattcta tctagaaaat tattattatt
     2641 aaaacaaagg tttgagttga taaatgtttg ataaaagaga gccgttggca tacagaatgt
     2701 gtccgagaac aattgatgaa tttgcagggc aggaggatat aatcgggaaa ggtaaattgc
     2761 tttacaggat gattaaagct gatagaataa ggtccattat attgtatggg ccaccaggta
     2821 ccggcaaaac atccctggca aaggttattg ctgctactac aaaatcccag tttgaaaagc
     2881 taaatgctgt tacagctggt gtagctgaca ttaaaaagat tgtggctgat acccataacc
     2941 aatttttaaa tccaaaaggg aaaacagttt tatttataga cgaaatacat agatttaaca
     3001 aagcccagca ggatgcactc ctaccttttg tagaagatgg tacaatagtt cttattggcg
     3061 ccacaacaga aaatccattt tttgaagtta ataaggcttt gatttccaga tcctcggttt
     3121 ttatgctcaa gcctctgatg gagaaagata tagaaaaaat tattcttaat gcacttcaag
     3181 atagagaaag aggtttaggc gaatacgatt tagaagttga taatgaagct gtaatttatc
     3241 tggctagcgt ttctaacggt gatgccagag ttgccttaaa cacaatagaa ttagctgtta
     3301 tgacgtcgga tatggataag gacggtaaaa tccatataaa tattaatact ataaaggaat
     3361 gttttcaaaa aaggcctttg gcttttgata aatcaggtga cagccattat gataacatca
     3421 gtgcttttat taagtctatg agaggcagtg atcccgatgc tgctgttttt tatcttgcaa
     3481 gagctattta tgggggtgag gatccggaat ttcttgcgag aagaattatt attgcagcct
     3541 ctgaggatgt tggcatggct aatcctactg ctcttcaagt agctgtggct gcagcagaag
     3601 ctgttaaaat gatcggaatg cctgaagcaa aaattatatt atctcatgca gctgttatgg
     3661 ttgcaaccag tcccaagtca aatgctgctt atattgctat atcaaaagca atggatgata
     3721 ttgagaataa acgtacaggt gaagtaccaa tgcatttgag aaatcctgtt actaaaggga
     3781 tgaaggagtt gggctatggc gaaggttaca aatatgctca cgattttaaa ggaaatattg
     3841 tagaccagga atacatgccc gaagaaatga gtggaacaat atattataac cctacaacta
     3901 atggttatga agcaaggata aaagaatggc tggataggcg a
//
LOCUS       DAOSDD010000336         3923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8149_length_3923_cov_3.486039, whole genome shotgun sequence.
ACCESSION   DAOSDD010000336 DAOSDD010000000
VERSION     DAOSDD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8149_length_3923_cov_3.486039"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /gene="secF"
                     /locus_tag="PK733_18580"
     CDS             <1..446
                     /gene="secF"
                     /locus_tag="PK733_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOJ12575.1"
                     /translation="VALAHDVLVMFSAYTIFNIPMNESFIAAVLTILGYSLNNTIVVY
                     DRIRENSNRMRKAPVDELVNTSITQSLSRTINTTATTLTAIIVVYIFAAANNIQSIKE
                     FAFPLIIGLISGTYSSLFVAPPLWMMWKQRQAKQKIVSKQAKVRS"
     gene            1066..1905
                     /gene="panB"
                     /locus_tag="PK733_18585"
     CDS             1066..1905
                     /gene="panB"
                     /locus_tag="PK733_18585"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOJ12576.1"
                     /translation="MKEKFTTADFKKNKINGKKITMLTAYDYPTAKILEEAGIDAILV
                     GDSLGMVVLGYEDTTKVTMDDMVHHIKAVSRGTKRSMVIGDMPFLSYHLGTHESVKNA
                     GRLVTEGGCSAVKLEGGKGVVDDIKAIINAGIPVMGHLGYTPQSVNLFGGHKAQGKTM
                     ETAREILNDALILQKAGVFSIVLECIPFKLAAYVSKKLDIPTIGIGSGAGCDGQVLVT
                     PDIIGLFKDFSPKHSKKYIDFGSQLEEAIKKYVEEVSNGAFPTNANSFIIDEGIIEHL
                     NIE"
     gene            2085..3014
                     /gene="panC"
                     /locus_tag="PK733_18590"
     CDS             2085..3014
                     /gene="panC"
                     /locus_tag="PK733_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOJ12577.1"
                     /translation="MKIIKYISEIKNIVKKEKISGKTIGLVPTMGYLHEGHLSLIRSS
                     VRDNEVTIVSVFVNPTQFGPNEDYNRYPRDLEKDAQHAENTGADILFAPSVEEMYPDG
                     YRTYINVEGITEVLCGRSRPGHFKGVATVVAKLFNITEADRAYFGLKDAQQFVAVKKM
                     VEDLNMNIEIIPCPIIREADGLAMSSRNAYLSLEEREAALVLSHSLFEAEAMVKKGER
                     DISHLLESIKRRISLEKLASVEYIEAVNADTLENIYKIEDKTKETAKSEINEMTKSEI
                     KEEIKSRNREKVLVALAIKIGTTRLIDNIIVEV"
     gene            3017..3373
                     /locus_tag="PK733_18595"
     CDS             3017..3373
                     /locus_tag="PK733_18595"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOJ12578.1"
                     /translation="MFITLMKSKIHRAVVTGSNINYVGSITIDRDLMDAAGLLENEKV
                     HVLNVSNGNRFETYIIPGEEKSGIVCINGAAARLVQPGDIVIIISYGLFDIKEALEFR
                     PNIVFVNEKNEITRTC"
     gene            complement(3360..3638)
                     /locus_tag="PK733_18600"
     CDS             complement(3360..3638)
                     /locus_tag="PK733_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12579.1"
                     /translation="MDIRTVISKKMKKIAIELSKLSSYTKVIIRWGVNIFLVFFALGT
                     LLIFRNRVTLCYDSNMEFIATSIIKASFTILAEVIIGGLFIDYAFNKS"
BASE COUNT         1422 a          534 c          790 g         1177 t
ORIGIN      
        1 ttgtcgcatt ggcacatgat gttctggtaa tgttttctgc atatactatt tttaatatac
       61 ctatgaatga atcctttatt gcagccgtac tgacaatact aggatattca ctaaataata
      121 caatagttgt ctatgaccgt ataagagaaa acagcaacag gatgagaaag gctcctgtag
      181 atgaacttgt aaacaccagt attacacagt ctcttagtag gacaataaat acaactgcta
      241 ctacactgac tgcaataatt gttgtctata tatttgctgc agcaaacaat atacagtcca
      301 ttaaggaatt tgcttttcca ttaataatcg ggctcataag cgggacatat tcttctcttt
      361 ttgttgcacc acctttatgg atgatgtgga aacagcggca agccaagcag aaaatagtgt
      421 caaaacaggc aaaagtaaga agctgacgaa tatgaaatgt attatgcatt tgtataatat
      481 actgaagttt tgcattttta ttataaaact tgattttaag ctatttatat ctaatcatga
      541 aagtaactga tttgaaggct ttcaacaaaa agtgcaaaac tatagtaata tggagactgt
      601 cctttttcat cgcttttgca tttgtacagc tggtaataat gtccgatagt atcacctgtg
      661 aactatacag cgcatatggt taattatata tgtttgattt gtgagtatga gaaataaaaa
      721 cctggaaaat aaaacaaatt taataactgc tttgatctat taatatggac agagacagga
      781 tgcaaattta atagcaataa tactaatttg tatgagaata gtttgaaacg catttatcat
      841 gccgtgttgg gtataaaatg cttaagttat ttgaaattcc ggcattctta tacaaagaga
      901 tttataattg taaagtttat taagctaata taaattggtc aatcctgttt gggaattggc
      961 cagtttttat tttacggcat tgaatcgagg acataaatcg aggacattaa aataaagaca
     1021 ttggatcgag taaaaccaat tcagctttga ttggaggtaa tataaatgaa ggaaaaattt
     1081 acaactgcag attttaaaaa aaataaaata aacggtaaaa aaataacaat gcttaccgct
     1141 tacgactatc ctactgcaaa aatattggaa gaagccggta tagatgctat attggtaggc
     1201 gattctctgg gaatggttgt attaggttat gaggatacta caaaagtaac catggatgat
     1261 atggtgcatc acattaaagc agtctcacgc gggacaaaaa gatctatggt aataggggat
     1321 atgccatttc tttcatatca tcttggtaca catgaaagtg taaaaaatgc aggaagactg
     1381 gttactgagg gaggatgcag tgcagtaaag cttgaaggag gaaaaggggt agttgatgat
     1441 ataaaggcta ttattaacgc aggtatacct gtcatgggac atttgggcta tacacctcag
     1501 tctgttaatc tttttggagg gcacaaagcc cagggaaaaa ctatggaaac agcaagagaa
     1561 atccttaatg atgctctaat acttcaaaaa gcaggagttt tttctatagt gcttgaatgt
     1621 ataccattta agcttgcagc ttatgttagt aaaaaactcg atatacccac aataggtatt
     1681 ggttcaggcg cagggtgtga tggccaggtt ctggtaacac cggatataat tggtttgttt
     1741 aaggatttct caccaaaaca ttctaaaaaa tatattgatt ttggcagcca gctggaagaa
     1801 gccataaaga agtatgttga agaagtaagc aatggtgcat tcccaacaaa tgctaattca
     1861 tttattatag atgaaggtat aattgaacac ttgaacatag aatagctaaa tatgggacat
     1921 ctgaacatag aacagttgga catggaacgg ttggacatgg aacagctcaa catagaatag
     1981 tcggatgcag aatagttaga tgcataataa ctgaatataa aacagggaga tgcagcttga
     2041 aagattaatt gcaatgcggt taccttgggg acatggaggt ttttatgaag ataattaaat
     2101 atattagcga aattaaaaat attgtcaaaa aagagaaaat atcaggtaaa acaattggct
     2161 tggtacctac aatgggttat ttgcatgaag gacatctttc acttataagg agttcagtgc
     2221 gtgataatga agttactata gtaagcgtat ttgtcaaccc cacgcaattc ggaccaaatg
     2281 aagactataa ccggtatcca agggatttgg aaaaggatgc tcaacatgcg gaaaataccg
     2341 gagcagatat tctttttgct ccaagtgtag aagagatgta tcctgatgga tatagaacat
     2401 atattaatgt tgaaggtata actgaagttt tatgtggaag gtctcgtccc ggacacttta
     2461 aaggagttgc aacagtagtg gcgaagttat tcaatattac tgaagctgat agggcctact
     2521 ttggattaaa ggatgctcaa caatttgttg ctgttaagaa aatggtagaa gatttgaata
     2581 tgaatataga gataatccca tgcccaatta taagggaagc agatggtttg gctatgagct
     2641 ccagaaatgc ctacctgtct ttggaagaaa gggaagctgc attagtctta tcacattctc
     2701 tttttgaagc agaagctatg gtaaaaaagg gggaaaggga catttcacat ttgttggaaa
     2761 gcattaaaag aagaataagt ttggaaaaac ttgcttctgt tgaatatatt gaagcggtaa
     2821 atgcagatac attggaaaac atatataaaa tagaagacaa aaccaaagaa acagctaaaa
     2881 gtgaaatcaa tgaaatgacc aaaagtgaaa ttaaagagga aataaaaagt aggaataggg
     2941 aaaaagtact tgttgcatta gctattaaaa ttggaaccac aagattgatt gataatatta
     3001 ttgtggaggt ataatcatgt ttataacttt aatgaagtca aaaattcatc gtgctgttgt
     3061 aaccggatca aatatcaatt atgtaggaag cataacaata gatagggatt taatggatgc
     3121 agccggatta ttggagaatg aaaaagttca tgtattgaat gtaagtaatg ggaataggtt
     3181 cgaaacttat ataataccgg gtgaagagaa gagtggaata gtttgcataa atggggcagc
     3241 tgccaggctt gttcagcctg gcgatattgt cattataatt tcttacggac tttttgatat
     3301 aaaggaggca ttggaattca ggcctaatat tgtttttgta aatgaaaaga atgaaataac
     3361 taggacttgt taaaggcgta atctataaaa agtccgccaa ttattacttc agctagtatt
     3421 gtgaaacttg cttttattat tgaggtagca ataaattcca tattagaatc atagcataaa
     3481 gttacccggt tgcggaaaat aagtaaagta cctaatgcaa aaaataccag gaatatatta
     3541 actccccatc ttattataac cttagtgtag ctactaagtt tacttaattc tattgctatt
     3601 tttttcattt ttttgcttat aacagttcta atgtccaaaa tataccccct ttccaaagaa
     3661 acagaagaat ttactgtttt atgttaacat aaaacagtaa attcttcaat aagaaaagtt
     3721 taccaaagaa aagtctaata aaaaagctgt tgtcgataga tggtatggtg tgacaagttt
     3781 atttttaaaa tgcattatgt tgcgtgaaaa ttgttatgtt aggtgacata attttacgta
     3841 aaacgtccgg ttaatatttt ataatagact catataccta aaacacaaat gataaagggg
     3901 gaaaaaatta atggttaaca aat
//
LOCUS       DAOSDD010000337         3922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8153_length_3922_cov_3.489268, whole genome shotgun sequence.
ACCESSION   DAOSDD010000337 DAOSDD010000000
VERSION     DAOSDD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8153_length_3922_cov_3.489268"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PK733_18605"
     CDS             complement(<1..113)
                     /locus_tag="PK733_18605"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HOJ12580.1"
                     /translation="MATGLDTDKIVSDLMKVERIPLDRLYQRKQIAEWRRD"
     gene            complement(166..558)
                     /locus_tag="PK733_18610"
     CDS             complement(166..558)
                     /locus_tag="PK733_18610"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOJ12581.1"
                     /translation="MKVNSMDAGNVRFSTVDEITRGSAIAKVTEVNMSKTESINKNAL
                     RLTEFERRELPVSERIVIDAIERANEAVSIANRRFEYSIHEKTKHIMVKVIDIETEEI
                     VREIPPEKILDLVAMIWEMTGLLVDERR"
     gene            complement(1178..1627)
                     /locus_tag="PK733_18615"
     CDS             complement(1178..1627)
                     /locus_tag="PK733_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ12582.1"
                     /translation="MFGLVPYRRSSSLRKRDNIWDIGSMLEDFFNDSLAPAFLTPVNP
                     MRADVRETEKEYIVDVEVPGVNKEDIKLDIKDDVLSICIEKNEEIKEERKDYIRRERR
                     YDACSRSFLVPDIKHEDVKAKYNNGVLTVTLPKADEVKDKKRKIDIQ"
     gene            complement(1866..3416)
                     /gene="spoVB"
                     /locus_tag="PK733_18620"
     CDS             complement(1866..3416)
                     /gene="spoVB"
                     /locus_tag="PK733_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HOJ12583.1"
                     /translation="MMSAKKSFISGAVVLMISGLVVRLLGFVYRIYISNLLGSEGMGL
                     FQLISPVYSLIILTLTSGVSIAVSKMTARETARRHFANIRRITLIALLAVFAAGTGIS
                     LLLLLNTGYIANSILKEPRTYYSILLLIPCIPVIALSSTLKGYFYGVQDVMPVAWSQI
                     AEQVVRIGLVLFTAKQFLDKGLEYACAFATGAMATGEIASLLVLYIMYKPSIRKYIKD
                     KSRKSPMRKRAILLEIFKVSLPVSSNRFITSVMSAVELILIPRRLLEGGMNYQESIEL
                     YGKLTGMAMPLIYFPCIVTSSLAITLVPAISEALSLKNFKTANYRISRSIQISFMLGF
                     TFSAIFLSYPNEISSIIYRKENIAELLYLLAFTCLFTYLQQTLAGILNGLGKQGISLR
                     NSVIGYIIRIGFVYFLLPVYGIRSYITAIFISSLLICALNLSTVIKATGMYIDIRNWI
                     LKPGAVGIFMLLVGKYIYSFFSIFIEHETLITVFSVAGNIIIGLLLMVTIGILEKEQI
                     LKMIGLKK"
     gene            complement(3488..>3922)
                     /locus_tag="PK733_18625"
     CDS             complement(3488..>3922)
                     /locus_tag="PK733_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12584.1"
                     /translation="DPLSEAECGHVLIGLGKMFEYNGEYDAVIDCFEKCMESGDPFVK
                     ETALRKLSQIYKRNNEYGKAIRHWEDMISNKEALCIFPLIELAKYYEHKAKDIPKAIE
                     MTNKAIELSLEVGLNNSIYRADLKKRLDRLMRKAENNNNARW"
BASE COUNT         1192 a          809 c          542 g         1379 t
ORIGIN      
        1 tcatcccttc tccattcagc tatctgtttt ctctggtata atctatccag gggaattctt
       61 tcaaccttca taagatcgct tactatttta tctgtatcaa ggcctgtagc cattcctcct
      121 atcctcaatc ttgaggtaaa caaattaata cccatatcca ttgcatcacc ttctttcatc
      181 aacaagcaat ccggtcattt cccatatcat ggcaaccaaa tccagtatct tttccggcgg
      241 aatttcccgc acaatttcct ctgtctcaat atcaataact tttaccatta tatgtttggt
      301 tttttcatga atagaatact caaaccttct gttagctata gaaactgcct cgttagccct
      361 ctcgatagca tcaattacaa ttctttcact tacaggcagc tctcttcttt caaattctgt
      421 aagccttaga gcattcttat ttatactttc agtttttgac atattaactt cagttacttt
      481 tgcaattgca ctccctcttg taatttcatc aaccgtactg aatcttacat taccagcatc
      541 catgctatta actttcataa aaacctccat gagtaaaact atacattgta actattggtc
      601 caaccaattg atattataaa catatcaact ttagcttaat aataatatcg gtaaatactc
      661 acagaaggtt taggcatttc tccagagata gctaaatttt ttttaattca ctcatagtag
      721 ggcgcgtttt ctttttatga agaaaatacg cctgccttca attgactata ccatatagca
      781 taacttctgc caggccactg atgtatggaa taaagaaaaa agcgggagat attgcccgct
      841 tttctattat tctatatagc attttattgg gacgcatttt ttgaagaaaa atatggcaaa
      901 tacagattgc cacgtatatg ttcacttcag caacgggttg taacgtttaa gtcttcaaga
      961 aaaattctat ccatcgcatt caccatccgt gggtcaggcg atggctcacg acatccgtgt
     1021 cgtgaattac gaatttttcc ttgccttaaa cataacaacc cgtgagcttt gttcaaatca
     1081 tatacttaaa aagcaaaaaa ggagcgttta ataatttttt gcttttggta atcgaacaat
     1141 gccatatttt ttaagttata tagaaaatgc acctatttca ttggatgtca attttccgtt
     1201 ttttgtcctt tacttcgtct gcttttggca atgttactgt aagaactccg ttattgtact
     1261 tcgcttttac gtcctcgtgc ttaatatccg gtacaaggaa acttctactg catgcatcat
     1321 atcttctttc tcttctgata taatcttttc tttcttcctt tatttcttca tttttttcta
     1381 tacagatact gagaacatca tctttaatgt caagtttaat atcctcttta tttactccgg
     1441 ggacttcaac atcaactata tattcttttt ccgtctccct aacatcagct ctcatgggat
     1501 ttacaggagt tagaaatgcc ggtgcaagtg aatcattaaa aaagtcttct aacatgctcc
     1561 caatatccca gatattatct ctctttctta aacttgaact tcttctatat ggaactaatc
     1621 caaacatgaa caacacctcc aaatatttta tttatgataa tctgtttgtc tttgactttc
     1681 tttgaccttc tatatattta tttatcatac atcgaaagca tatgcaatgc cggttttaag
     1741 ttattacttg cctttatcag ttaatattcc ttaatatgtg tattttccta cgattttttc
     1801 attaaatctt cattctacta tattaactca tttttctaac gcatttatct aactcatttt
     1861 taaattcatt tcttcaaacc gatcattttt agtatttgtt ctttttccaa tatacctatt
     1921 gttaccataa gcaataaacc aataataata ttgcctgcca ctgaaaaaac agtaattaat
     1981 gtttcatgtt caataaatat ggaaaaaaaa ctgtaaatat atttacctac caatagcata
     2041 aatattccta cagcaccggg ttttaatatc cagtttctta tatcgatata cattcctgtt
     2101 gcctttatta ctgttgataa atttaacgcg catattaaaa gtgagctgat aaatatagct
     2161 gtaatataac tccttatacc ataaacaggc agtaaaaaat acacaaatcc gattcttatt
     2221 atatagccta taactgaatt tcttagagat atgccttgct tacccagtcc attcagtatt
     2281 cccgccagtg tctgttgaag atacgtaaaa agacaggtaa atgccaggag atagagaagt
     2341 tcagcgatat tttcttttct ataaataata ctacttattt cattgggata ggataaaaat
     2401 attgcagaaa aagtgaatcc cagcataaaa cttatctgta tggacctgga aatcctataa
     2461 ttagctgtct taaagttttt taatgacaat gcttctgaaa tagctggtac caatgttatt
     2521 gcaagggatg atgtaactat gcatggaaaa tatataagag gcattgccat tcctgtcagt
     2581 tttccatata attctatgct ctcctgataa ttcatccctc cctctaaaag ccgccttggt
     2641 atgagtatca actcaacagc agacataact gatgtaataa acctgtttga agacaccggc
     2701 aaggagactt taaatatttc cagtaaaatt gcccgtttcc tcatcgggct ttttctggat
     2761 ttatccttaa tatactttct tatactgggt ttatacataa tgtataatac caataagctt
     2821 gctatttcac ctgtagccat ggcccctgtt gcaaaggcgc atgcgtactc taacccttta
     2881 tccagaaatt gttttgctgt aaatagtaca agaccaatcc ttacaacttg ttctgctatt
     2941 tgagaccatg caacaggcat tacatcctgc acaccataaa aatagccctt taaagtggaa
     3001 gacagagcga taaccggtat acaaggtata agtaataata tggagtagta agttcttggt
     3061 tcttttaaaa tactatttgc aatataaccg gtatttaata gaagcaaaag agaaatgcct
     3121 gtacctgcag caaaaactgc taacaaagct attaaagtta tccttctgat attggcaaaa
     3181 tgcctcctgg ccgtttcacg cgccgtcatc ttggatacag ctatagatac accggaggta
     3241 agtgttagta taataaggga atagaccgga gatataagct gaaaaaggcc cattccttct
     3301 gaacccagta aatttgatat atatatcctg taaacaaagc ctaataaccg gacaacaagc
     3361 ccagaaatca ttaaaactac agcaccgctg ataaatgatt ttttagcaga catcatgacc
     3421 tctcttttaa taagtatcat gatataatta ttaatttgca ggcatacata tgacaattat
     3481 ttatattcta ccatcttgca ttgttattat tctctgcctt cctcatcaat ctatccaatc
     3541 tctttttcaa gtcagctctg tatatactat tgtttaatcc cacctccagg gacagttcga
     3601 tcgctttgtt tgtcatttct atagctttag gaatatcttt tgctttgtgc tcataatatt
     3661 ttgccagttc tatcaaagga aatatacata gtgcttcctt gtttgatatc atatcttccc
     3721 agtgtctgat agctttccca tactcattgt tccttttgta tatttgagaa agctttctta
     3781 atgctgtttc cttcacaaaa ggatctcccg attccatgca tttttcaaaa caatcaataa
     3841 cagcatcata ctcaccattg tattcaaaca tttttccgag accaataagt acatgtccgc
     3901 attcagcttc tgaaagcggg tc
//
LOCUS       DAOSDD010000338         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8169_length_3917_cov_6.228897, whole genome shotgun sequence.
ACCESSION   DAOSDD010000338 DAOSDD010000000
VERSION     DAOSDD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8169_length_3917_cov_6.228897"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK733_18630"
     CDS             complement(<1..448)
                     /locus_tag="PK733_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12585.1"
                     /translation="MKCRKLSPIGVIALVLTLIFIGCVLCSTSGTQSNSVSSNDVNNE
                     GKATQKEETKEDMDIVNVKDFGAKDFIEPVECKLDETDIVIAVNGIGNISVGDNVRIS
                     NGVKTGIPGVDLTAKITAIEGNKITLDRKSYSSITTKLYIDSTDAFK"
     gene            complement(577..2874)
                     /locus_tag="PK733_18635"
     CDS             complement(577..2874)
                     /locus_tag="PK733_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12586.1"
                     /translation="MKNKKISKIISIMIIISLSLILCVSCSPDNGIKVDNDSKNASSS
                     SINTPDTAKDKIDENKDKTKGNQDMVSVAEYGAKDFVEPIECKLDETDVVIATNGIGN
                     LCVGDNVRITCGVKTGDPGVDLLAKVTAIDGKKITLDRKSYSSITTKLYIDSTEAFKK
                     AFDYANSDDIDVLIPEGNYYVDSQVNKKVVIQTNVKCEGKIYTTNKGTVPLFTISRKE
                     EPIFINGSSIAGSLEAGTKRIPEIEKYVDYDVIFKSKEILMPRNNNIGEYYTKNEANR
                     INSDGSLMVEMAESYNNKNLLQMSLHKKERALKLENLNVLCIDKALTNLGKHIIEVGR
                     SDVTFENLLLDNENLQKENGQHTGVLICEAVNVTINNSKIRGFMRQGLGYPVRPEVAL
                     YVTLNNTDISYARHAVSGMCDNYTTINGGSFEAYGGVIDTHWGHNLTVNNAKISSDST
                     PFCVDGGKLTINNCDITTGHYMMINRRMDTPSLTDVEIKDTKVKYTGTANFSLVHGQA
                     TQYDHNDASLYNPNLTVENVRIEIINPIPLVDIYYVYTPMDKKYTMQKLYERIRVKDL
                     YITGKDDAKLKNTYFRMMPSTSKYYWEGNPDIYLENIKVNRDANSVNNYDGLIAYYLH
                     PDNTEVKNTHFNMEVRNCGTLSFEIVPAILGDMLIENCKIVKYSYYGDLLKDWSNNPV
                     WWQKGTGKTVFKNVKFDRTGLSPSSIYIMNDAEFENCEFTNFGAIDYMNFGKQLPAVK
                     ILKVKDCIFNDGGITGGSLSEYIKP"
     gene            complement(2892..3761)
                     /locus_tag="PK733_18640"
     CDS             complement(2892..3761)
                     /locus_tag="PK733_18640"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ12587.1"
                     /translation="MKISAFSVCAPEYDVRGTIEVLKELGYDAVEWRTTNMPPISKPD
                     TYNIQNRYWSYNLSTVDINRIDEIADEIGIMCKNAGLEICSFSTYLNQNNIDDINKVA
                     NAANIAGCKNIRLALPGYNKNENYRTQFNRAIKEVQPLEKIADRYKVRINFEMHPGNI
                     IPSASAAYRFVSNFDPKYLGVIYDPGNMIFEGYEDYRMGIELLGEYLAYVHIKNAAWK
                     HEGFTKDGAWIWKPSNAPTKRGCADLPGLINILKEKDYEGYLSIEDFSNEEDTYTKLK
                     NNLEYLKKLTSNK"
BASE COUNT         1104 a          798 c          519 g         1496 t
ORIGIN      
        1 ttttaaaagc atcagtacta tctatatata attttgtagt tattgaactg taacttttcc
       61 tgtcaagagt tattttgtta ccttcaatag ctgtaatttt agctgttaaa tctacaccag
      121 gtatcccggt ttttacccca ttggaaattc taacattatc ccctacgctt atattaccaa
      181 ttccattgac agcaataact atatcagttt catcaagttt gcactctact ggttcaataa
      241 agtccttagc tccaaaatcc tttacattca ctatatccat atcctctttt gtttcctcct
      301 tttgtgtagc ttttccttca ttattaacat cattactact aacactgtta ctctgtgttc
      361 cacttgtaga gcataaaaca cagccaataa aaatcaaagt taaaacaagc gcaattactc
      421 caattggcga aagttttcta catttcacaa attttcctcc tcttgataca cccagaagca
      481 tctgaattat ttttgtcaac caatccactg agcttgatac cttcaaaaaa ctttttttaa
      541 aataaggtaa ctgtattcct gttcatctat tacttgttaa ggtttaatat attcacttaa
      601 actacctcct gtgataccac catcgttaaa tatacagtcc ttaactttga gtatctttac
      661 tgcaggcagc tgtttcccga aattcatata gtctatggct ccaaagtttg taaattcgca
      721 gttttcaaat tcagcatcgt tcataatata tattgaagaa ggtgaaagtc cggttctgtc
      781 gaattttaca tttttgaata ctgttttgcc tgtacccttt tgccaccata caggattatt
      841 ggaccaatct tttaataagt ctccataata actgtatttt actattttgc aattttctat
      901 gagcatatct cccaatattg caggtacgat ttcaaatgat agtgttccgc agtttctcac
      961 ttccatattg aaatgtgtat tcttaacttc tgtattatct ggatgcaagt aatatgcaat
     1021 taagccatca taattattaa cactgttggc atctctgtta accttgatat tttccagata
     1081 aatgtccgga ttaccttccc agtagtactt gcttgtggaa ggcatcattc taaaatatgt
     1141 attttttaat ttggcatcat cttttcctgt gatatataag tctttcaccc ggattctttc
     1201 atataatttt tgcatagtat actttttgtc cattggagta tatacataat atatatccac
     1261 caaaggtata ggatttatta tttctatcct tacattttct actgttaaat tagggttgta
     1321 taaactggca tcattatggt catactgtgt tgcctggcca tgtaccagac tgaagttggc
     1381 agtaccggta tatttaactt ttgtatcctt tatttctaca tcagtcaaag aaggtgtatc
     1441 cattcttcta tttatcatca tataatgtcc tgtagttata tcgcaattat taatggttag
     1501 cttacctcca tcaacacaga aaggcgtact gtcggaagaa atcttagcgt tatttaccgt
     1561 aagattatgt ccccagtgtg tatcaattac tcctccatag gcttcaaaac ttcctccatt
     1621 aattgttgtg tagttgtcac acattccgga aactgcatgg cgtgcatagc tgatgtcagt
     1681 gttatttaag gttacataaa gagcaacttc cgggcgcaca ggataaccaa gaccctggcg
     1741 cataaatcct cttatttttg agttgtttat ggtaacattt acggcctcgc atattaacac
     1801 acctgtgtgt tgtccatttt ctttctggag attttcattg tctaataaaa gattttcaaa
     1861 tgttacatcg cttcttccga cttcaataat gtgtttacca aggtttgtaa gagctttatc
     1921 aatacatagt acattaagat tttccagttt taatgctctt tcctttttat gcagactcat
     1981 ctgcagtaaa tttttattat tataactctc agccatttcc accataagag agccatcact
     2041 attaatcctg ttagcttcgt tctttgtata atattctcca atgttattat tccttggcat
     2101 aagtatttct tttgacttaa atattacatc ataatctaca tatttctcaa tctcaggtat
     2161 tctctttgta cctgcctcaa ggcttccggc aatactgctt ccgttaataa atatcggttc
     2221 ttctttgcgc gaaatagtaa atagggggac agtcccttta tttgttgtat atatctttcc
     2281 ttcacatttt acattggttt gtattactac ttttttatta acctggctat ctacataata
     2341 attaccttca ggtattaaca catcaatatc atccgagttt gcataatcaa aagctttttt
     2401 gaaagcctcc gtgctgtcta tatataattt ggtggttatt gagctgtaac tctttctgtc
     2461 aagagttatt tttttgccat ctatagctgt aactttagcc aataaatcca cacctggatc
     2521 accagttttc actccacagg ttattcttac attatcacct acacataaat tgccaatccc
     2581 attagtagca ataactacat cagtttcatc aagcttgcat tctattggct caacaaaatc
     2641 ttttgctccg tattccgcca cacttaccat atcctgattt ccctttgttt tatccttatt
     2701 ttcatctatt ttatcctttg ctgtatcagg agtattaatg ctgctgctgc tggcattttt
     2761 actgtcatta tctaccttta ttccgttatc aggcgagcat gaaacacaca aaattaacga
     2821 taaacttata ataatcataa tagagataat tttagaaatc tttttgtttt tcacttgaat
     2881 cctcctcttc attatttatt agaagtcaac tttttcagat attcaagatt atttttcagt
     2941 ttagtatatg tatcttcttc attagagaaa tcttcaatag ataaatatcc ttcatagtct
     3001 ttttctttca atatattaat aagccccgga agatctgcac atcctctctt tgttggtgca
     3061 tttgacggct tccatatcca ggctccgtct tttgtaaaac cttcatgctt ccatgctgca
     3121 tttttaatgt gaacatatgc aaggtactca ccaagtaatt caatacccat tctataatcc
     3181 tcatagcctt caaaaatcat attacccgga tcataaatta ctcctaaata ctttggatca
     3241 aaattggata caaatctgta tgctgcactg gcactgggaa ttatattgcc aggatgcatt
     3301 tcaaagttaa ttctgacttt atacctgtca gcaatctttt caagtggctg tacttcttta
     3361 attgccctgt tgaactgtgt cctgtaattt tcgtttttat tatatcctgg caaagctaat
     3421 ctgatgtttt tacatccggc aatattagct gcattggcta ctttattaat atcatcaata
     3481 ttattttgat taagatatgt agaaaatgag caaatttcca gaccggcatt tttacacata
     3541 attcctattt catcagcgat ttcatcaatt ctatttatat ccaccgtact caaattatat
     3601 gaccaatatc tgttttgaat gttatatgta tccggctttg atattggagg catattggta
     3661 gtcctccatt ccaccgcatc atatcccagt tcttttaaaa cttcaattgt cccgcgaaca
     3721 tcatattcag gagcacatac actaaaagca ctgattttca taatattttc ttttctcctt
     3781 tttaaataat tacaacaaat attatatatt catattcctg ttcagattcc cgatcatggt
     3841 ttcatatact cacttaaatt acctccagta atgccaccat cattaaattt gcagtctttt
     3901 actttaagta tctttac
//
LOCUS       DAOSDD010000339         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8207_length_3903_cov_7.989605, whole genome shotgun sequence.
ACCESSION   DAOSDD010000339 DAOSDD010000000
VERSION     DAOSDD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8207_length_3903_cov_7.989605"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK733_18645"
     CDS             complement(<1..189)
                     /locus_tag="PK733_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOJ12588.1"
                     /translation="MKMIMAVISDEDSNPVVSELSQAGFGVTRLCSTGGFLRAGNTTI
                     MIGTDENRLNEAIDILRVK"
     gene            complement(283..657)
                     /locus_tag="PK733_18650"
     CDS             complement(283..657)
                     /locus_tag="PK733_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HOJ12589.1"
                     /translation="MAGSMKGIVAYRRKQEEDALVKAEKAIVELQFMGKAINFNSVSK
                     QSGVSKSFLYGDEQMKERIEQLREKTVNKEMNKRAKYDKTAKSQNVVIEAKEKRIAKL
                     EEENRKLKVEVEHLRGLLYSMK"
     gene            complement(657..2630)
                     /locus_tag="PK733_18655"
     CDS             complement(657..2630)
                     /locus_tag="PK733_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12590.1"
                     /translation="MMLNNGALALKEQATGRYGEILSYLSEDDGYWIENDVWEVQNKA
                     FTDNGLFPYLRKTATKIDFSHYVNEMIRNEAKYYLLSSLKKGDLKLGNVLNIYSMPIK
                     RLGDYLSASNIREIEELKGDEVFIRYLNQHYAGRKGCRKNWNHYLAFKNGFMEFIRDF
                     YDEREEMEKDVWHAAKLPGVRISACDRRGRCSISFADIPEYYRPMVKRYLGKLITRRS
                     WSYCSEMLVYLKYFFGIFYRHGHENGFLENLCRADIEKYLIWTMEEHKDSNATYRSKA
                     VSFIRYFLDYIQMAEFPLAPEKDVGRLIFNDDIPRRERISDTMEKVKYIPEPVKEQLD
                     AAITDIEPKEMMIVYVLLRETGWRGTDVLNLRYNNCLDYLWNKVEARYVPYLCGEITK
                     TGIPRLKIPIRDEVEKMVRELAEEAREKSTDDNNPEKYFFNTYEGRNMGMPISKLAFV
                     QAVKELIERKGITDASGELYHFKTHALRHTRALEYAEQGMPIGVIQQILGHCSLQMTL
                     HYAKISENALYEKWKATERLKLFHVVGAEPPKVSSKRPQEESIRYEFVRKTLDAVRVP
                     FGTCFKPSKIACKHQMEKCLECPNFCSEKDNIPEYEAEIKRVEKLIGLSQYISRKDWE
                     EKNTEYLELLKKILERIRKEGIVHKNGQFREGM"
     gene            complement(2617..3747)
                     /locus_tag="PK733_18660"
     CDS             complement(2617..3747)
                     /locus_tag="PK733_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12591.1"
                     /translation="MEVVKVRNEDNHIRYYLNDDNGEPIEEILKFLKFKDNTGYARNT
                     LRMYCTHLKQYYEYLAERGKSYREVNIDDLAGFVAWLQNPYLGQKVVPIRYENARLPQ
                     TVNKTVDTVVAFYEYLARYDEYEKNLPAALVKFVKAPGRNYRSFLYGIADSKPVKSHV
                     LKLKTPRQEIRTVKKENIPSLLNACTNLRDYLLVYMLFETGMRIGEALSLWLEDFDMS
                     ECIIHIRDRGEMENLSEIKTVSSPRRLDMTQELSDLFLRYVCEYHTEEVRTNHVFIKL
                     RGKSMGKAMDYADVDNLFRTLCRKTGVEITPHMFRHTSLSMLYSAGWEPELLRKRAGH
                     KNIYTTLNTYVHPTDEEVTEAFRKAIETIQCPLGSMGVHDVE"
BASE COUNT          945 a         1001 c          651 g         1306 t
ORIGIN      
        1 tttaacccgc aatatgtcaa ttgcctcgtt cagcctgttt tcgtcggttc caatcattat
       61 agtagtatta cccgctctta aaaatccccc tgttgaacat aaccttgtta caccaaaacc
      121 cgcctgtgac agttcactga cgaccgggtt gctgtcttca tcgctaatta ccgccattat
      181 catcttcatg ttgccttacc tccggaatta atctcgtggt caggggcgtc cagcccctga
      241 aacacggcat ttcttacagc gactacatgt agtcgctgtt gactatttca tactgtatag
      301 caaacctctc agatgctcta cctcaacttt tagttttctg ttttcttcct caagctttgc
      361 aatacgtttc tcttttgctt cgataactac attctgtgat tttgctgtct tatcgtattt
      421 agccctttta ttcatttctt tgtttactgt tttttcgcgg agctgttcaa tgcgttcttt
      481 catctgttca tcgccataca gaaaactttt tgacaccccg ctttgctttg aaacactgtt
      541 gaaatttatg gctttaccca taaactgaag ctcaactatg gctttttctg cctttaccag
      601 ggcatcttct tcctgtttcc tcctataggc cactatgcct ttcatgcttc cagccatcac
      661 ataccctccc ggaattgccc atttttatgg acaatccctt ctttacggat acgttccaat
      721 atctttttta acagttccaa atattctgta ttcttttcct cccaatcctt gcggctaatg
      781 tattggctga gtccgattag tttctcaacc cgcttaattt cagcctcata ttcaggaata
      841 ttatcttttt cactgcaaaa atttggacac tccaagcatt tttccatctg gtgcttacaa
      901 gctatctttg acggtttaaa acatgtcccg aaaggcaccc ttaccgcatc cagagtcttc
      961 cttacaaatt catatcgtat gctttcttcc tgtggccttt tggaagaaac ttttggcggc
     1021 tctgcgccca ccacatggaa tagcttcagc ctttctgtag ctttccattt ctcataaagg
     1081 gcattttcag aaatttttgc atagtgcaag gtcatctgca agctgcagtg cccgagtatc
     1141 tgctgaatga cgcctatcgg catcccctgt tctgcatatt cgagagccct tgtatgtctt
     1201 aatgcatgcg ttttgaaatg ataaagctcc cctgatgcat ccgttattcc tttcctttcg
     1261 ataagctcct tgacggcctg cacaaaagcc agtttgctaa ttggcatccc catatttctt
     1321 ccttcatagg tattaaaaaa gtacttttcc gggttgttat catccgtgct tttttctctt
     1381 gcttcctctg caagttccct taccatcttc tccacttcat cccttattgg aatcttaaga
     1441 cgtggtatcc cggtcttggt tatttcaccg caaaggtagg ggacataacg cgcttcaacc
     1501 ttgttccaaa gataatcaag acaattgtta tacctcaggt tcagcacatc cgtaccacgc
     1561 caacctgttt ccctgagcaa cacatagacg atcatcattt cctttggttc tatgtccgtg
     1621 atggcggcat caagctgttc cttgaccggt tccggtatat atttcacttt ctccatggta
     1681 tcggaaatcc gttcgcgtct tggaatgtcg tcattaaaga tcagcctccc gacatctttc
     1741 tctggtgcca gtggaaattc agccatctgg atataatcca ggaaatatct tatgaatgac
     1801 acagctttgc ttctgtaggt tgcattactg tccttatgtt cttccatcgt ccatatcaga
     1861 tacttttcta tatccgccct acaaagattt tcaagaaacc cattctcatg accatgtctg
     1921 taaaatatcc cgaaaaaata cttcagatat accagcatct cactacagta agaccaactc
     1981 ctgcgtgtga tcagttttcc gagatatctc ttcaccattg gccggtagta ctccggaata
     2041 tcagcaaagg atatcgaaca ccttccccgt ctatcacagg cggatatcct tacccccgga
     2101 agctttgcag catgccagac atccttttcc atttcctctc gctcatcata aaaatcacgg
     2161 ataaattcca taaaaccgtt tttgaaggca agataatggt tccagttttt acggcatcct
     2221 tttcttccgg cataatgctg attcagatat ctaatgaata cttcatctcc tttgagttct
     2281 tctatttcgc ggatatttga tgcactcaaa tagtcgccca gcctttttat cggcatgctg
     2341 tatatattca gcacattacc cagtttaagg tctccctttt taagcgaact cagcagataa
     2401 tacttcgcct cattccgtat catttcgttc acataatgac tgaaatcaat ctttgttgca
     2461 gttttccgta aatacggaaa cagtccattg tccgtaaatg ctttattctg tacttcccat
     2521 acatcatttt ctatccaata gccatcatct tcggacagat aggaaagaat ttcaccatat
     2581 ctgccggtag cctgctcctt tagcgcaaga gctccattat tcaacatcat gcacccccat
     2641 acttccaaga gggcattgaa tggtctctat agcctttctg aatgcttctg tgacttcctc
     2701 gtctgtagga tgcacatacg tgttcagtgt tgtatagata tttttatggc ctgctctttt
     2761 ccttaacaat tccggctccc aaccggcaga gtagagcatg ctcagcgatg tgtgccggaa
     2821 catgtgaggg gtaatctcca caccggtttt tctgcagagg gtacggaaca ggttatccac
     2881 atccgcatag tccatagctt ttcccataga tttgccccga agctttatga atacatggtt
     2941 tgttcggact tcttccgtat ggtattcaca tacataccgc aggaataaat cggacagctc
     3001 ctgtgtcatg tcaagccgcc tcggacttga aaccgttttt atttcagaaa gattttccat
     3061 ttcacccctg tcccggatat ggatgataca ttctgacata tcaaagtctt ccaaccataa
     3121 tgataatgct tcaccaatac gcattcctgt ttcgaacagc atgtatacaa gcagataatc
     3181 ccttaaattc gtgcatgcat tcaacaggga gggaatattc tcctttttca ctgtacgtat
     3241 ttcctgtcgg ggtgttttga gctttaatac atggcttttg acaggtttgc tgtctgcgat
     3301 tccatacaga aagctacgat aattccgccc gggcgcctta acaaacttaa ccaatgccgc
     3361 aggaagattc ttttcatact catcatacct tgcaagatac tcatagaatg ctactactgt
     3421 atcaactgtc ttgtttactg tctgcggcag tcttgcattt tcataccgga tgggaactac
     3481 tttctgtccc aagtatgggt tttgcagcca tgcaacaaat cccgccagat catcaatgtt
     3541 tacttcccga tagcttttcc ccctttctgc taaatactca taatactgtt tcaaatgtgt
     3601 acaatacatg cgcaacgtat ttctggcata tcctgtattg tccttgaatt ttaagaactt
     3661 caggatttct tcaatgggct ccccattgtc atcattcaga taatatctga tatggttatc
     3721 ctcattcctt acttttacta cttccattgt ctgaacccct ttctattgag atacagacaa
     3781 tgtatatacc atttagattt gtttttcaac agcgactact gcgactactg cgactactat
     3841 cacgagattc cactaattta tcgccttaaa accttacagc gactactgcg actactatat
     3901 ttc
//
LOCUS       DAOSDD010000340         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8296_length_3863_cov_5.867910, whole genome shotgun sequence.
ACCESSION   DAOSDD010000340 DAOSDD010000000
VERSION     DAOSDD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8296_length_3863_cov_5.867910"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3863)
                     /locus_tag="PK733_18665"
     CDS             complement(<1..>3863)
                     /locus_tag="PK733_18665"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ12592.1"
                     /translation="ESMDSTGVKPDSSYMLKSQNNITLDKIKNLLSIDGEPAPEIIQK
                     DHNLFRITPSSPLEQNKLYVFRIKQEDGYDITWTFQTSAMFKITSSFPDDKTTNVPVN
                     TGIEIYFSHEEYEDPSNYFEIIPKVKGSFERHKKALVFVPQELKKATLYTVKIKKGVK
                     LANTDYELKDDYVFMFETSADENEENGNTYKGYFSYNRLLNEYTPNEIPHLPFNFYLR
                     QEKVKKNSIKINTQVYAYSSLESFINAIAEKDKVPSWAYYSYVKNQIPVKGLQKVLEF
                     QQEFNNPSHGEQFIKLPQNLAEGYYLVNSNWDDITFQTLMQITDVGMYLMQAEEKTLI
                     WLNDLSTGKPVEGAEITIAGGNVEMHGNNQNSIKFYSNEDGIAYFDTPKDIHVTDEEQ
                     QQNYYQPSYYTERKYFKIITKDNKAAVLGYSSYIYNNGRYSTNYYWRFLHTDRNLYKP
                     DDTVCFWGFIQNRYEGKNIDKLTVEVTKSNYRPMAYMYAEDGGRRKSYIPPFTGNQPL
                     VKQIVELKNGVFTGELQLPFLEPGSYLLVLKNDRNIIINSTYIQIKNFTKPPYKLDIT
                     KDKEAVFPGQEANFNIKASFFEGTGVPDLAINYNIGSSTGENSISGNPTTDSTGSVSI
                     KYTPQPPSQAQGENQAYFTCHSTLPEIGAINQHDNLRVFVNDINVKLESSFINNVAII
                     NAELNRITLDRINSGTANDHWDYLGEPVSGKALNGTIFKNTWVKIEDGQYYDYINKVT
                     RKSYRYELKTETVKDNFVLTTGLDGKAKFEYPLPEITDGYYSAKVYCVDNSGRDMKFD
                     TYFSSYPYYRYSYYDDNRYFLDGVKENYKIGETVELTYKKGRDILPKDGSYLFIKAQN
                     GIKDFVLSSKSDYSTVVEEKDIPNIHIIGVYFNGITYVQSEYCNIVFDKKERDLVIEA
                     SSDKEAYKPGEEAIVKVNIKDKSGKPQKSAINISVVDEALFKLREQYVDTLDTLYMQI
                     SAGISNTYSSHQNSGVDIIGGTGMDGAERTKADNGALAMEKSSKSPSMANMALADVAF
                     GGAGSDYLREIFKDTAFFKTIETDENGYGEYRFKLPDNVTSWRITLSGLTAGLFAGTN
                     KTSLNVTLPFFINYTLNSTYLAGDKPVLGVNAYGSSLKKDETVYFEVRSSKDPNKFAK
                     ADGKAFERVNIPLWEFAEGKDDLIIKAFTINGSSDSIKHRVLTVNTYHQMEEAIYYNL
                     EPGLVFEGGKTGNTRLIFSDKSTGAFLPELVSLSYTSGSRIDQRLSAKLASNIIEKYF
                     KEEDITNIINLINRDESFKPSEYQV"
BASE COUNT         1067 a          721 c          683 g         1392 t
ORIGIN      
        1 tgcacctgat attcagatgg tttaaagctt tcatccctat ttatgagatt aattatgtta
       61 gtaatatctt cttctttgaa atatttctca ataatgtttg atgccagctt tgcagagagc
      121 ctctggtcaa ttctgctgcc tgaggtataa gacagagata caagctcggg aaggaaggca
      181 ccggtgcttt tgtccgaaaa tatcagacgg gtattgcctg ttttaccgcc ttcaaaaact
      241 aatccgggtt ctaaattata ataaatagct tcttccattt gatgataggt atttaccgtt
      301 aagacacggt gctttatgga gtccgacgag ccatttatag taaaagcttt aataatcaga
      361 tcatctttgc cttctgcaaa ttcccagaga ggtatattta ctctttcaaa agctttgcca
      421 tcggctttgg caaacttatt ggggtcttta gaacttctca cctcgaaata aactgtttca
      481 tcttttttaa ggctgctgcc ataggcatta actccaagca caggcttatc cccggcaaga
      541 taggtagagt taagagtata atttataaag aaggggagag ttacattaag actggtttta
      601 ttggtccctg caaataaacc agctgtaaga ccggacaggg ttatccgcca ggatgttacg
      661 ttatcaggta atttaaacct atattcccca tagccatttt catctgtttc aatagttttg
      721 aaaaatgctg tatctttaaa aatttctctc agataatcac tgcctgctcc cccaaaagcg
      781 acatcggcta aagccatatt tgccatactg ggagatttag agcttttctc catagctaga
      841 gcaccgttat ccgcttttgt gcgttctgct ccgtccatac ccgtgcctcc aataatatca
      901 acacccgaat tctgatggga actgtaggtg tttgatatac ctgctgatat ttgcatataa
      961 agagtatcaa gagtatctac atattgttcc cgaagtttaa agagtgcttc atcaacaaca
     1021 cttatattga tagccgactt ttgaggctta ccggacttat cttttatatt aacctttaca
     1081 atagcttctt cgcctggttt ataggcctct ttatctgaag atgcttcaat tacaaggtct
     1141 ctttcttttt tgtcaaatac tatgttgcag tattcagatt ggacatatgt tattccgtta
     1201 aaatatacac ctattatatg gatattggga atatcctttt cttccactac ggtagagtaa
     1261 tcagacttgc tgctcaagac aaaatccttt ataccatttt gggccttgat aaaaaggtaa
     1321 ctgccatctt ttggaaggat atcccttcct tttttgtacg ttaattcaac agtttcacct
     1381 attttataat tttcctttac tccatcaagg aagtacctgt tatcatcata atatgagtac
     1441 ctataatatg ggtaacttga aaaatatgtg tcaaacttca tgtctcttcc ggaattatca
     1501 acacaatata cctttgcgct atagtatccg tcggtaattt ccgggagtgg gtactcaaat
     1561 tttgctttgc cgtcaaggcc tgttgtcaat acaaaattat cctttaccgt ttctgttttt
     1621 aattcatatc tatagctctt gcgtgtaact ttgtttatat aatcataata ctgaccgtct
     1681 tcgattttta cccaggtatt tttaaatata gttccattca aagcttttcc tgaaacaggc
     1741 tcacccaggt aatcccaatg gtcatttgca gtgccgctgt taattcgatc cagtgtaatc
     1801 ctgttaagtt cggcattaat tattgctaca ttatttataa atgatgattc taattttaca
     1861 tttatgtcat ttacaaatac tcttagatta tcatgctggt ttatagctcc tatttccgga
     1921 agtgtggaat ggcaggtgaa gtatgcctga ttctcacctt gagcctgtga gggcggctgt
     1981 ggggtgtatt ttattgatac tgaacctgta gaatcagtag tcgggttgcc ggaaatcgaa
     2041 ttttcaccgg tactgctgcc gatattataa tttatagcaa gatcaggcac tcctgttcct
     2101 tcaaaaaaag atgctttaat gttaaagttg gcttcctggc cagggaacac agcttcttta
     2161 tctttagtta tatcaagctt gtaaggcggt ttcgtgaagt tttttatttg aatataggtg
     2221 ctattaataa taatattgcg gtcatttttt aacacaagca gataactgcc tggttcaaga
     2281 aaaggcagtt gcagctcccc ggtgaacaca ccatttttta gttctactat ttgttttact
     2341 aaaggctgat ttcctgtaaa tggtgggata taactttttc tgcgacctcc atcttcagca
     2401 tacatataag ccattggacg ataattgctt ttggttactt ctacagtaag tttatcaata
     2461 tttttccctt catatctatt ttgaataaat ccccagaaac agaccgtatc gtcaggttta
     2521 taaagattac ggtcggtatg caaaaatctc cagtagtaat tggttgaata tctaccatta
     2581 ttatatatat aggaagagta gccaagcaca gcagctttat tatctttagt tattatttta
     2641 aaatattttc tttcggtata gtatgaaggc tggtagtagt tctgttgctg ttcttcgtct
     2701 gtaacatgga tatctttggg tgtatcaaag tatgcaatac catcctcatt ggaatagaac
     2761 ttaatgctgt tttggttatt gccatgcatt tccacattgc cgccagctat tgttatctct
     2821 gctccttcaa cgggtttccc cgttgaaagg tcatttaacc atataagagt tttttcttct
     2881 gcttgcatta aatacatacc tacatcggta atttgcatta gtgtctggaa ggtaatgtca
     2941 tcccaattgc tgttaacaag atagtaccct tcagccaggt tttgcggcag ttttataaac
     3001 tgttcaccat gtgaaggatt attaaattcc tgttgaaatt ccaaaacttt ttgcagtcct
     3061 tttacaggaa tctggttttt cacataactg taatatgccc aacttggtac tttatctttt
     3121 tctgcaatgg catttataaa ggattccaaa ctgctgtatg catagacctg agtatttatc
     3181 ttgatagagt ttttcttgac cttttcttgc cgtagataaa aattgaaggg aaggtgtggt
     3241 atttcgttgg gggtatattc attaagaagc ctattgtaac taaaataacc tttataagta
     3301 tttccgtttt cttcgttctc atcggctgat gtttcaaaca taaatacata atcgtctttt
     3361 agttcataat ccgtatttgc cagttttacc ccttttttta tctttacagt ataaagagtg
     3421 gcttttttca attcctgggg tacaaatacc agtgcctttt tatgtctttc aaagctgcct
     3481 ttcaccttag gaataatttc aaaataatta gaaggatctt catattcttc atgactaaag
     3541 tatatttcta tacctgtatt tacaggtaca ttagtggttt tatcatcagg aaaagaagaa
     3601 gtgattttaa acatggcact ggtctgaaaa gtccaggtta tatcataacc atcttcctgt
     3661 tttatcctaa acacatacag tttgttttgt tcaagtggag aagatggtgt aattcgaaat
     3721 aaattatgat ctttctggat gatttcagga gcaggttctc catctattga tagcaggttt
     3781 tttattttat caagtgtaat gttattttga gacttaagca tataactgct gtcaggttta
     3841 acacctgtag aatccataga ttc
//
LOCUS       DAOSDD010000341         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8337_length_3851_cov_4.555585, whole genome shotgun sequence.
ACCESSION   DAOSDD010000341 DAOSDD010000000
VERSION     DAOSDD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_3851_cov_4.555585"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK733_18670"
     CDS             complement(<1..322)
                     /locus_tag="PK733_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12593.1"
                     /translation="MDAKSFKGNRWIIISGKYEGVEKYAVDELYKLVQQHVPYILTVY
                     AGSTASEGLKDELKEDLREDLREGLKSDLKNYNIIFIGTEESNEYIAGLLKDGIIRLN
                     KDDDT"
     gene            complement(347..1444)
                     /locus_tag="PK733_18675"
     CDS             complement(347..1444)
                     /locus_tag="PK733_18675"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12594.1"
                     /translation="MTSRERVEAALNHKQPDRTPIFEYVLLSPVADIVIGHKYMDYGG
                     NYRGWLNMAEETGWERALRQYVSDRLELAEKLGHDMLYVVPNPPPGILKQEIAADKKS
                     GNEMLQETSDPVEKLLRRNELNRQTIGKFDEDRFLVYYYLFEEMKKRGIDLPIKAPAY
                     VHGIWTDVDLMQTMVLEPEAAHEHFKLATERAIPDIKKYLEIGIKQIGIGGDFSGNRL
                     LISPESYKCFIVPHLKILSDMIHQYGGWAINASDGNLWPVIDDFLVNSGVDGYIEIDM
                     RAGMELKKLKTEYGHMITLYGNMDCGEVLSFAEPEEIRNYTIECIENGWSNGGHIFTA
                     SNAITGSVKLENYMSMVNAYRDIFNLPPLKV"
     gene            complement(1765..>3851)
                     /locus_tag="PK733_18680"
     CDS             complement(1765..>3851)
                     /locus_tag="PK733_18680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ12595.1"
                     /translation="SMVEHFHNTNEYEKNPGIISGKMGHIIKKGILNIDGLNIHYSVN
                     VNAYRLIRIKQTKYLLISIIMNIILIIVISQHQAKRIIKPVTDLNQAISIFPNNDQGI
                     ECEPNNKCKKTVSLHERLTQYFIGTTVIPFILFICACYISSIGELKNYITNYSDSIVE
                     TEAKTISKIIERKEKSFFMFINEPFVLEAMMHKNDRKEDVGAALDKGVFMGIGKSRVL
                     LMDTSGEILLYNNKTIIDNLKKYIITSETSLTSETPDTSGAYKCFLPVKWKMDRNLLE
                     EFTLMLDVPVFCSEGSKDMKFVGIAAIFIDLWEIENIFYRSNTIFRRAYLIDHTGRVF
                     DVYNKAYISEDESIALSIPKFGNDNKYGIHIIKDIKGLDWQVVSIFSKDYINSKTNSL
                     KRDCIYCLFIVILLSIVYSFFISNQLLKPINRLNYYFNTVKLDSISDINIDEFYIDEI
                     DKIGKNFITMMERIEQLIDELMLSQYNNLALEKIGRKAQIKALQAEITPHFLYNTLDI
                     IINLIRDIKTEKAVSMIQALSDLFRYSITDNEYSTITIGEELRYAESYANILAVHYNE
                     QVRFIWDIEPDIEQYDCMRMFLQPLLENCVVHALNGNDLEVTIKCKVSGEDIIFIVQD
                     NGKGIEEIELVKLRNRIKAENCHDAHIGLNNINSRLKLLYGDLYNLNVDSKRGIGTTI
                     VIKIPAKIGYVP"
BASE COUNT         1182 a          769 c          499 g         1401 t
ORIGIN      
        1 ctgtatcatc atctttattc aacctgatta taccgtcttt taacagcccg gctatatact
       61 catttgattc ctcagtacct ataaatatga tattataatt ttttaaatcg ctttttaacc
      121 cctctcttag atcttctctt agatcctctt ttagctcatc ttttaaacct tcagaggctg
      181 tactgcccgc ataaaccgta agaatatatg gcacatgctg ctgaaccagt ttgtacagct
      241 catcaaccgc atatttttct actccttcat atttgcctga tataataatc catcggtttc
      301 ctttaaagct ctttgcatcc atacatatcg tctccttttc tattttttat accttcaagg
      361 gcggcaggtt aaaaatatct ctgtatgcat ttaccatgga catataattc tcaagtttta
      421 cgctgccggt tatggcattg cttgcagtaa aaatatgtcc cccgttactc catccgttct
      481 ctatgcattc aatggtataa tttctgattt cctcaggttc ggcaaaactc aagacttccc
      541 cacaatccat gttgccatat aatgttatca tgtgtccata ttctgtttta agctttttta
      601 actccatccc tgcacgcata tcgatctcta tgtatccatc tactccacta tttacgagaa
      661 aatcatcaat aacaggccat aaattgccat cactcgcatt tatagcccat cctccgtact
      721 gatgtatcat atccgaaaga attttaagat gtggtactat aaaacactta taactttccg
      781 gagaaatcaa aagcctgttt cctgaaaaat cgccacctat tccgatttgt tttataccaa
      841 tttccagata ctttttgata tcaggaattg ccctctctgt tgccagctta aaatgctcat
      901 gtgccgcttc aggttcaaga accatagttt gcataagatc cacatcggtc catatgccat
      961 ggacataggc aggtgccttt ataggtaaat cgattcccct ttttttcatc tcttcaaaca
     1021 gataataata taccagaaat ctgtcttcat caaacttccc gattgtttgc ctgttaagtt
     1081 catttcttct aagtaacttt tcaacaggat ctgatgtttc ctgaagcatt tcattgccac
     1141 tctttttatc tgctgcaatc tcttgcttaa gaattccggg aggcgggtta ggcactacat
     1201 aaagcatgtc gtggcctaac ttttccgcaa gttccagcct atctgaaaca tattgcctta
     1261 aagcacgttc ccatcctgtt tcttctgcca tatttagcca acctctataa ttaccgccat
     1321 agtccatata tttatgtcct ataacaatat cagctacagg ggataacaga acatactcga
     1381 atatcggcgt tctgtcaggc tgcttatggt ttaatgctgc ttctactctc tctcttgacg
     1441 tcatcactaa tacctccgca aaatcaaata tacttgtaca tgaaaattta tggtctgtat
     1501 ggaaattgcg tccgcaatat taataacagt attcaatagt aataaatact gttattaaat
     1561 actacaatat ataattacta tgtcataaaa gataaattaa ctcaaggtaa tattctaatg
     1621 aaaaattaaa ctattctaat atttgattct gttgcaaaaa ctcatttagt gtcaacattt
     1681 tgaaaatggg gacactcttc agcgtaaaag aatacttctt gcttgagaga agagtgtccc
     1741 cttaccttag actcgcttgc aattttatgg cacatatcct atttttgcag gtatttttat
     1801 tactatagtt gtacctatac cccgtttact gtcaacatta aggttgtaca aatctccata
     1861 taacagtttt aatcttgaat ttatgttatt aagtccgata tgagcatcat gacaattttc
     1921 tgccttaatt ctatttctta actttacgag ttcaatttct tctatcccct ttccattatc
     1981 ctgaactatg aatataatat cttctcctga tactttacac tttatagtga cttcaaggtc
     2041 atttccatta agcgcatgaa caacacaatt ttccaaaagc ggctgaagaa acatccgcat
     2101 acaatcatac tgttctatat caggttcaat atcccaaata aagcgtactt gttcgttata
     2161 atgaacagct aatatatttg cataagattc ggcatatctt aattcctctc caatagttat
     2221 agtggagtat tcattatcag ttattgaata cctgaataaa tcactcaatg cctgaatcat
     2281 tgaaactgcc ttttctgtct ttatatctcg aatgagattt attattatat ccaaagtgtt
     2341 atacagaaaa tgaggtgtaa tctctgcttg tagcgcttta atctgtgctt ttctgcctat
     2401 tttctccaat gccagattgt tatattgcga cagcataagt tcatctatca gttgttcaat
     2461 tctttccatc attgtaataa agtttttacc tatcttatca atttcatcaa tgtaaaattc
     2521 atctatatta atatcactta tagaatccag ttttacagta ttaaaataat aattcaagcg
     2581 gtttattggt ttcaaaagct gatttgatat aaaaaatgaa taaactatag acagtaatat
     2641 tacaataaac aaacaataaa tacaatccct ttttaatgaa tttgtttttg aattaatgta
     2701 atccttcgaa aaaattgaaa caacctgcca gtcaaggcct tttatatctt taataatgtg
     2761 tatgccatat ttattatcat tgccaaattt cggaatactt aatgcaatac tttcatcttc
     2821 cgatatgtat gccttattat aaacatcaaa tactcttccg gtatgatcaa ttaaataagc
     2881 tctcctgaaa attgtatttg acctgtaaaa aatattttct atttcccata aatcaataaa
     2941 aatagctgct atcccaacaa atttcatatc tttcgatcct tctgaacaga aaacaggaac
     3001 atccagcata agggtaaatt cctcaagcaa attacggtcc attttccact tcacaggtaa
     3061 aaagcattta tatgcacctg aagtatccgg agtctctgaa gttaatgaag tttctgatgt
     3121 tataatgtat tttttcaaat tatcaataat tgttttatta ttatacaaca ggatttcacc
     3181 tgatgtatcc attagcagaa ccctactttt tcctattccc ataaaaactc ccttatccag
     3241 tgcggctccc acatcttcct ttcgatcatt cttatgcatc atagcttcca aaacaaacgg
     3301 ctcatttata aacatgaaaa aggatttctc ttttctttcg ataatttttg atatggtttt
     3361 agcttcagtt tctacaatac tatctgaata atttgtaata taatttttga gttcacctat
     3421 agaagatata taacatgcac agataaataa gataaaaggt atgacagttg ttccaataaa
     3481 atactgtgtt aacctttcgt gaagagaaac tgtctttttg cacttattat taggctcaca
     3541 ctctatacct tggtcattat tgggaaaaat agatattgcc tgattaagat ctgtaacagg
     3601 ttttattatt ctttttgctt ggtgctgaga tataactata atgagaataa tattcataat
     3661 aatagaaatt aataaatatt ttgtttgttt tattctaatt aatctataag catttacatt
     3721 aacactataa tgtatgttta gaccatctat atttaatata cctttcttaa ttatatgtcc
     3781 cattttgccg ctgataatac ctggattttt ttcatattca tttgtattat gaaaatgctc
     3841 taccatagat t
//
LOCUS       DAOSDD010000342         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8345_length_3848_cov_5.455049, whole genome shotgun sequence.
ACCESSION   DAOSDD010000342 DAOSDD010000000
VERSION     DAOSDD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8345_length_3848_cov_5.455049"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PK733_18685"
     CDS             <1..888
                     /locus_tag="PK733_18685"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOJ12596.1"
                     /translation="SLQGIPDSINVVTVCDREADIYDFINKVLLEGKHFLIRVAQNRL
                     IVGEHNLLKSEVEASPVAGEILVQIPRDIENEKPPREAKLSVRYCSVIIKPPLHRKSD
                     KTLSNLKLYAILAKEADHPKDSAKPIEWLLLTSHSTSSLEDVIEKINWYKQRWKIERF
                     HFTLKSGCKIEDRQFETVERLYNVLVIYSIIAWKLLFLSYKSRTNPDIRCDIILKKYE
                     WKALYSMVNKMSTPPDTPPTLKEAVLLLSKLGGFLARKSDGDPGVKTLWRGMQRLEDI
                     SQLWLVIHPEDYSNDMGKE"
     gene            complement(1159..2481)
                     /locus_tag="PK733_18690"
     CDS             complement(1159..2481)
                     /locus_tag="PK733_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12597.1"
                     /translation="MSNFKAGYARLDITPPLGVNISGYYEQRIADGILDNLYASAVAV
                     SDGSNTAVVISADVIGIPQNKMNVYRKSIAERNSIPFEAVFIACTHTHTGPVIGNDLF
                     PEDPNYNDYLQRRLSDVTTLAIEDLKPASVSVGRSAAPGISFIRRFRMKDGKIQTNPG
                     VGNQNILEPIGIPDETVQLARLHRDNAKEILLVNFQVHPDVIGGNKFSADYPQFVRET
                     LENALDNVHCIYFNGAQGDTNHINVFTTPDKANGGYEHSKHMGRCIAGAVLQIYTKTT
                     PVSSDQIRFGQQTIEVPSNRVDKSLIPDAEKIIALHEAGRDTDIPFKGMELTTVVAEA
                     YRMKRLENGPDAFDLHLTAISFGNVTIAGIPGEPFTDIGRGIKNGSPFEMTFVCCCAN
                     GYEGYYPMQSAYDEGGYEARSSKFKPGVAEAIIAGSIGLLKKLKKCIN"
     gene            complement(2647..3789)
                     /locus_tag="PK733_18695"
     CDS             complement(2647..3789)
                     /locus_tag="PK733_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12598.1"
                     /translation="MNPTDKQKQKIRQTIGVCRFVYNFYISHNKEVYEKEKRFVSGMD
                     FSKWLNNDFISNNPEYKWIKDVGSKAVKQSIMNAEQAFKRFFKGQSKFPHFKKKKNQD
                     VKTYFPKNNKTDWTVERHRIKIPTLGFVQLKEKGYIPTNKKVTSGTISYKAGRYYVSV
                     LVDEDIKVLNNNTNEGIGIDLGVKDLAIVSNIDKPFKNINKTKKTKKLEKKLKREQRK
                     LSRKYESLKIRNKKEGGNATRQNIQKQVVKVQKLHSTLTNIRDNYANQVVNEIVKTKP
                     SYITIEDLNVSGMMKNKHLARAIAQQKFYQFKTRLQSKCVLNGIELRIVDRFYPSSKM
                     CSECGAIDKTLNLSDRTYICKECGCVIDRDKNAAINLANAKTYKIA"
     gene            complement(3776..>3848)
                     /locus_tag="PK733_18700"
     CDS             complement(3776..>3848)
                     /locus_tag="PK733_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HOJ12599.1"
                     /translation="KYKNKIKEDEEIAKSLQNRDKSH"
BASE COUNT         1152 a          717 c          613 g         1366 t
ORIGIN      
        1 agcttgcagg gtattcctga tagtattaat gtagttacgg tgtgtgaccg tgaagctgat
       61 atatatgact ttatcaataa agttttacta gaaggaaaac attttctaat aagagttgct
      121 caaaacaggt taattgttgg agaacataat ttgctaaaat ctgaagttga agcgagtccg
      181 gtagctggag aaattctagt ccaaattcca agagatatag agaatgaaaa accacctcgt
      241 gaagctaaac ttagtgttag atattgttca gtgattatta aaccaccttt acaccgtaaa
      301 agcgataaaa cattatcaaa tttgaaatta tatgcaatat tagccaagga agctgaccat
      361 cctaaggatt cagcgaaacc aatagaatgg ttattattaa catcacattc aacaagttcg
      421 ttagaagatg taatagaaaa gattaattgg tataaacaaa ggtggaaaat agagcgtttc
      481 catttcactt taaaaagcgg ttgtaagatt gaagaccgac agtttgaaac tgttgaacga
      541 ctttataatg tactcgtgat atattcaatt atcgcatgga agctcctttt tctctcttat
      601 aagtcaagaa caaatccaga tatacgatgt gatattatcc ttaaaaaata tgagtggaaa
      661 gctctttata gtatggtcaa taaaatgtct acccctccag acactcctcc tactttgaaa
      721 gaagctgttc ttcttctatc taaattaggt ggatttttag ctagaaaaag cgatggagat
      781 ccaggagtta aaacactctg gcggggtatg caacggttgg aggacatttc tcaactgtgg
      841 ttagttattc atccggaaga ctattccaat gatatgggta aagaatagat taaaatagag
      901 aggattcccg ctgtgtttcc taaaaatact tttttcaaga atttaaaaat ctttatacat
      961 agaatttatc ttctacatat aaagattttt tatttacatc gcatattcaa ttaacctata
     1021 gttatcttat actacagtta tcctgcagta ataaattaaa cccactattc tgaaatagca
     1081 actatagtaa cttgtactat taaattatgt aaactgatac tgcttcagtt tatactttat
     1141 tgctttgact tcatacattc aatttataca ttttttaagc ttcttaagta aacctataga
     1201 acctgcaata attgcttcgg caacacctgg cttgaatttg gagctgcggg cttcatagcc
     1261 tccctcatca taagccgatt gcataggata atatccctca tagccatttg cacagcagca
     1321 gacaaaggtc atctcaaatg gagagccatt ctttatgccg cggccaatgt cggtaaacgg
     1381 ctcaccgggt ataccggcaa tggtaacatt gccaaagctg atagctgtaa gatgcaggtc
     1441 aaaggcatca ggtccatttt caagccgttt catgcggtat gcctcggcaa ccactgttgt
     1501 aagctccatt cctttaaacg gaatatcagt atcccgtcct gcttcatgta atgcaattat
     1561 cttttcagca tcgggaatca atgatttgtc aacgcggttt gaaggaactt caatagtctg
     1621 ctggccgaat cttatctgat ccgatgatac aggagtagtt ttagtataaa tttgaagtac
     1681 tgcaccggct atacatcttc ccatatgctt tgaatgctca tagcctccat ttgccttatc
     1741 gggagtagta aatacattta tatggtttgt atcaccttgt gcaccattga agtaaataca
     1801 atgaacatta tccagagcat tctcaagggt ttcacgtaca aattggggat aatcagcaga
     1861 aaatttatta ccgcctatta catcagggtg gacctggaaa ttaacaagta gtatttcctt
     1921 tgcgttatca cgatgcaagc gcgctaactg gacagtttca tcaggaattc cgattggctc
     1981 cagaatattc tgattgccaa cacctggatt tgtctgaatt ttgccatctt tcatacggaa
     2041 acggcgtata aaagaaatgc caggtgcagc actgcgccca acactcacag atgcaggctt
     2101 taaatcttct attgccaaag ttgtcacatc acttaaccgc ctttgaagat aatcgttata
     2161 attaggatct tcaggaaata aatcattacc tattacaggc ccggtatgtg tatgcgtaca
     2221 tgcaatgaat acagcctcaa aaggtatgga attgcgctca gctattgatt ttcggtaaac
     2281 attcatctta ttctgtggaa tacctataac atcagcactg attacaacag ctgtattaga
     2341 accatcactg acagccacag ctgaagcata taaattatca agtatgccat cagctatacg
     2401 ttgttcgtaa taaccggaaa tgtttacacc aagcggcggt gttatatcaa gacgtgcata
     2461 acctgcttta aaattgctca tattatgtag cctgctactc aaaatatgga caaacatacc
     2521 catattgaga aatttcctta ttcatcgtgt ctgttctcgg atattactac cctactaaca
     2581 tttggtttaa cactccaaag gcttaaactc ccgactagcc atcggtacat acgtatattg
     2641 gcttgattat gctatcttat acgttttcgc atttgcaaga tttattgctg cgtttttatc
     2701 cctatcaatt acacatccac attctttgca tatatatgtt ctgtctgata gattaagtgt
     2761 tttgtcaatt gctccacatt cactacacat tttacttgat ggataaaatc tatctacaat
     2821 tcttaattct ataccattta gaacacattt gctttgaagc ctagttttga attgataaaa
     2881 cttttgttgt gctattgctc ttgctaaatg cttattcttc atcatcccac ttacatttaa
     2941 atcttctata gttatataag atggtttggt ttttactatc tcattaacca cttgatttgc
     3001 ataattatct cttatgttcg taagtgtact atgaagtttt tgtaccttga ctacttgttt
     3061 ttggatattt tgtctagtag catttcctcc ttctttttta tttcttattt ttaaactttc
     3121 atatttcctt gaaagttttc tttgctcacg ttttaatttc ttttccagtt tctttgtttt
     3181 cttagtttta ttaatatttt taaatggttt atcaatatta cttacaattg ctaaatcttt
     3241 tactcccaag tcaattccta tgccttcatt ggtattattg tttaatactt ttatatcttc
     3301 atctaccaat actgaaacat aataccttcc tgctttatag gaaattgttc cacttgttac
     3361 ttttttattg gttggaatat aacctttttc ttttaattga acaaatccta atgttggtat
     3421 ttttatccta tgcctttcta cagtccaatc agttttatta tttttaggga aataggtttt
     3481 tacatcctga ttctttttct tcttaaagtg tggaaattta ctttgtcctt tgaaaaacct
     3541 tttaaaagcc tgttcagcat tcattattga ttgctttacc gccttactac caacgtcttt
     3601 aatccatttg tattcaggat tattagaaat aaaatcattg tttaaccatt tggaaaaatc
     3661 cattcctgaa acaaatcttt tttctttttc ataaacttct ttattatgag agatataaaa
     3721 attataaacg aatctacata cacctatagt ctggcgaatt ttctgttttt gtttgtcagt
     3781 gggatttatc tcggttttgt aggcttttag caatctcttc atcctccttt atcttatttt
     3841 tatatttt
//
LOCUS       DAOSDD010000343         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8440_length_3817_cov_5.231526, whole genome shotgun sequence.
ACCESSION   DAOSDD010000343 DAOSDD010000000
VERSION     DAOSDD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8440_length_3817_cov_5.231526"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(599..1867)
                     /locus_tag="PK733_18705"
     CDS             complement(599..1867)
                     /locus_tag="PK733_18705"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOJ12600.1"
                     /translation="MNKSKYNPYEEMLEVLEKSAKMLGLQPNEYEALKSPERELKVSI
                     PIEMDDGTVKVFEGYRIQHSSSRGPCKGGIRYHQDVNLEEVKALAAWMTFKCAVANIP
                     YGGAKGAVKVNPRELSRNELMRLTRRYTAMILPLIGPEKDIPAPDLNTNPEIMGWVMD
                     TYSMFKGYTVHGVVTGKPIEIGGSLGRKEATGRGIMLIVREILNCMGVPMQGARIAVQ
                     GMGNVGGTAARLLYNQGCKIVAVSDISTGLYCESGLNINDIAEYVGSNGGGNLLDGYH
                     ADNVKRVSNDELLTCDVDVLIPAAMENQINENIARQLKANIVVEAANGPTTLDGDKVL
                     EERGIVVVPDILANAGGVIVSYFEWVQNNQAILWNEERINNSLEEMLTNAFNEVWTKA
                     KAKNTTLRMGAYMVALERLVKAKKLRGMFP"
     gene            complement(2415..3668)
                     /gene="nagA"
                     /locus_tag="PK733_18710"
     CDS             complement(2415..3668)
                     /gene="nagA"
                     /locus_tag="PK733_18710"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOJ12601.1"
                     /translation="MEHIEKVKIINGNIITPFKIIKNGCITIENGKITGILDEKADKA
                     DKSDKSDKADKCCRSGNNDCSNVNIIDAGGKYVAPGFIDIHTHGGGGHDFLDATVEAY
                     LGAAEKHIQHGTTALVPTTTTSTNEELKETFAVYKKAKKENKKGSQFLGLHLEGPYFA
                     MSQKGAQDPRFIRSPKREEYEKILEWTDDIIRWSAAPELDGALEFGRLMKEKGILPSI
                     AHTDAIYEDVLEAFENGFTHVTHLYSATSMVRRINAYRYAGVVEAAYLIDEMTVEIIA
                     DGSHLPASLLKLIYKIKGADHVALVTDSIRAAGQPEGESIIGSLKNGMPVIVEDGVAK
                     LPDRTAFAGSVATADRLVRNMVNLADVPLIDAVKMMTLTPASIIGVSSRKGSIVAGKD
                     ADIVIFDKDINIDMAIVGGQVLYKK"
BASE COUNT         1077 a          826 c          560 g         1354 t
ORIGIN      
        1 gtattcatct ggaggacact cccatgacaa aagccatgtt ttgtcaatat tatatttttt
       61 catgttttca agaaatctgt ttaaatcatg tccatgccaa tctgcatgat tatgaacatc
      121 gataatcatt attacatctc cttaatgcag tcatatttta ctacatttaa tattataaca
      181 tattataata tataagaaaa atggctgcta caaaataatc tttgcgttac aattgtaact
      241 ccccttccgg tagcttctct tctacaaaga gaacctccaa cttcaatagg ctttcctaaa
      301 gttgttttat agcatgttac acatttatta tgcttttgtg aaaatattat tattgtatac
      361 attatgctcg gcttatgttt tttaaagtat aagactccat tttatgcaac accgtatctt
      421 atttcctgca tttgcaactt tgtttttatg tccttcatta caacatttac aacatattcc
      481 ttaacaaaaa aaataaagcc attgtacaga atgattatgc aatcttactg tttaatggct
      541 tactgtatgt taatagattc tatgttctaa ttatatataa tccgaggtac aaaccttctt
      601 atgggaacat tcctcttaat tttttagcct ttacaagcct ctcaagggca accatatatg
      661 cacccattct taaggttgta tttttagcct ttgcttttgt ccaaacctca ttaaaagcgt
      721 tggtaagcat ttcttcaagg ctgttattta tcctttcttc attccataaa atcgcctgat
      781 tgttctgcac ccattcaaaa taagatacta taacacctcc ggcattggca aggatgtcag
      841 gaacaacaac tatccctctt tcttcaagaa ctttgtctcc atctaaagta gtgggaccat
      901 ttgccgcttc aacaacaatg tttgctttaa gctgtcttgc aatgttttca tttatctgat
      961 tttccatagc tgccggtata agcacatcta catcgcatgt taacaattca tcatttgata
     1021 cgcgttttac gttgtcagca tgataaccat ccaaaaggtt tccgccgcca ttgcttccta
     1081 catattctgc aatatcgttt atatttaatc ctgattcaca atacaacccg gtagaaatat
     1141 cactgaccgc aacaatctta catccctgat tgtataaaag cctggccgca gtaccaccta
     1201 catttcccat accctgcact gcaattctgg ctccctgcat tggtacaccc atacaattga
     1261 gtatttctct tactattagc attatacctc ttcctgttgc ttctttcctg cctaatgagc
     1321 cgcctatctc aataggttta cctgtaacta ccccatgtac ggtataaccc ttgaacatgc
     1381 tgtaggtatc cataacccat cccattattt ctggattggt atttaagtcc ggagcaggga
     1441 tatctttttc aggtcctatt aaaggcagga tcatcgctgt gtatcttctt gtaagcctca
     1501 ttaactcatt tctggatagt tccctgggat ttacttttac agcacctttg gcacctccat
     1561 aagggatatt ggcaactgca catttaaaag tcatccatgc tgccagagct tttacttcct
     1621 ctagatttac atcctgatga tatctaatac cgcctttaca tggtcctctg gaacttgagt
     1681 gttgtattct gtaaccctca aagactttta cggttccatc atccatttct ataggaatag
     1741 atactttcag ctccctttca ggacttttaa gagcttcata ttcgttaggc tgaagcccta
     1801 gcatttttgc cgatttctcc aggacttcaa gcatttcttc atatggatta tacttgcttt
     1861 tattcatata aaaattaacc cccttagcta tactaaatca tatatattaa ttattagatc
     1921 agttagcagc ttaatattga aacaattata atacttaaat taaaaacaat caactgtttt
     1981 ttgaaaaatt taattttttt attgcaaaaa agaattatta tattttttta ttgcatatta
     2041 ctttagggac tatgtttggg agcaacacga aaatttaatg cgtttaatat ggttataata
     2101 caagctggca gcgtgttata ctcatattac attatatccc ttgatttact taaaaggtaa
     2161 ctcctgcgct ttttagatgt gaaaacagat tttggaaaat attttttttg cagtttttta
     2221 tttcattact atcattttga ttaataagtc aggggacact cctctctcga acggagcgta
     2281 tttcttttac tttgaatact ttgaagagcg tatctgctgt taaacagcaa aaatccctgt
     2341 acttattagt gttgctccaa agtacaggga tttaattttt gtttataggc aatagcaagg
     2401 tttagccact agtgctattt cttatataat acctgaccac caactatagc catatcaata
     2461 ttaatatctt tgtcaaatat aacaatgtcg gcatctttac cagcaacaat acttcctttt
     2521 ctgctgctta ctccaataat tgaggctggt gttaaggtca tcattttaac agcatcaatc
     2581 agaggcacat ccgcaaggtt caccatattc cttaccagcc ggtcagctgt agctacactt
     2641 cctgcaaatg cagttctatc aggcagtttt gcaactccat cctcaacaat tacaggcatt
     2701 ccatttttca agcttccaat aatgctttct ccttcaggtt gtccggcagc acgtatggaa
     2761 tcagttacca gagctacatg atcagctcct ttgattttat aaataagttt cagcaaactc
     2821 gccggaaggt gtgatccgtc agcaataatc tcaacagtca tttcatcgat taagtatgca
     2881 gcttctacaa cacctgcgta tctgtatgca tttattcttc ttaccattga tgtggctgaa
     2941 taaagatgag ttacatgagt aaatccattt tcaaaagctt ccagtacatc ctcatatatt
     3001 gcatcggtat gggcaataga tggaaggatt cctttttctt tcatcagcct tccaaattca
     3061 agtgcaccat caagttcggg cgctgcactc caacgaatta tgtcatcagt ccattccagt
     3121 atcttttcat attcttcacg ttttgggctc ctaataaatc tggggtcctg agcacctttc
     3181 tgagacattg caaagtatgg tccctctaga tgcaatccta gaaactgcga acctttctta
     3241 ttctcctttt ttgccttctt gtatacggca aaggtttctt taagctcctc attggtgctt
     3301 gtggttgtag taggtacaag tgctgtagtt ccatgctgga tatgcttctc tgctgcgcca
     3361 agatatgctt ccacagtggc gtcaagaaaa tcatgtcctc caccgccatg tgtatgaata
     3421 tcaataaatc ctggtgcaac atatttgccc ccggcatcta ttatatttac attgctgcaa
     3481 tcattattcc cactacggca gcatttatca gccttatcag acttgtcaga cttatcagct
     3541 ttatcagcct tttcatccag tatcccggta attttcccat tttcaattgt tatacaacca
     3601 tttttaataa ttttaaaagg tgttattata tttccattaa taatctttac cttttcaatg
     3661 tgttccacac atttcacctc ttattcaaaa aataactaca tgatgtatta atattaataa
     3721 ccctaaagct gcattgattg cctactaact caattattcc ggggccagtt ggcaaaatac
     3781 atgtattcaa gattctcatc agaaatgcag gtcatat
//
LOCUS       DAOSDD010000344         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8451_length_3814_cov_4.891194, whole genome shotgun sequence.
ACCESSION   DAOSDD010000344 DAOSDD010000000
VERSION     DAOSDD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8451_length_3814_cov_4.891194"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3814)
                     /locus_tag="PK733_18715"
     CDS             complement(<1..>3814)
                     /locus_tag="PK733_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOJ12602.1"
                     /translation="GKEEVLYIINQYVRERGAVYSSFIEHLLNKLKKQGRKGTAALIY
                     VNEKLHDQGLKSDEVASFEHQVQAAAQVSIGNSLTSLKFISSMDWSEIFESLSHVEQI
                     LKQDPDGIYIEMDFESRNYYRQAIGKLAELCNVSETVIALKALECAKEQDIPQYRHIG
                     YYIIGKGRKNLLLKLKHKLAPFRYFDEIIKMKPEAFYIGSIAFITLVITGLFIYYAVY
                     EGIKTNTRLVATAVAAGIAVLIPASEIAVALVNTLVSKILRPSLLPKLQLKDGISEEL
                     STMVIVPTLLPNEKRVIELVDQLEVHYLSNKENNLYFALVGDFKDGNLEIALEDKKII
                     NKALDGVERLNEQYAPEGKKIFFYFHRKRKHNSVQNRWMGWERKRGAIVELNDLLQGS
                     PDTSYTVLSCEQDEIPSVKYIITLDADTKLPIGAAKKLIGTLAHPMNKAVIDQDKGIV
                     TEGYGILQPRINISILSCSISPFTRIFAGQGGIDLYTTAVSDVYQDLFGEGIFTGKGI
                     YDLNIFQTVLKNAIPENAILSHDLLEGCYIRAGLVTDVDLIDGYPARYNSFSMRLHRW
                     VRGDWQLLPWLSSRVRNKEGVIVNNPISTVCKWKIVDNMRRSLMNPSLLLLLFLGLLI
                     LPGSACIWVGLAVLASGIYTLIQFIRTLLAGDFFKYRQGKYATGISPLKAGLYQTILQ
                     LIFIPYQAYLMIDAIVRTIFRLFFTKRNMLEWVTAADMEASLKNDTVSFFRRMWMAPL
                     AGVILLLGSLAIDMNINMNKVIPSFVLAVLWASSFYLAYILSKPYETKAEKLKDAEIL
                     LLRKTARKTWGFFEDTVTAKDNYLPPDNLQEEPYKGVAHRTSPTNIGLLVTSTIAARD
                     LGYISCYDMLQLLDNIITTLEKMEKWEGHLYNWYNTTTLKVLRPAYVSAVDSGNFVGY
                     LIVASEALKELLRNKETENPCVNINMAYGLKDTFSIVKEEVNMPNIKLYEELIDYYIE
                     KYHSTDNMDLKEWEKILNMIASELEAIKADSKLKRYSWIKKLYRMTTSFMKELDAMRV
                     HAQSTDKNDNIAANDNAINNDNAINNDNAINNDDAINNDNAASNGNVVNDDNAMTYLC
                     RCLIQRINLLIENTKFAPLFDEKRQLFYIGYDGEKNRFSKSYYDLFASEARQTSYIAV
                     ARGEVDVKHWFRQGRRLTMVDEYRGLVSWTGTMFEYFMPLLIIRNYENTLLDETYRFV
                     VAAQKRYGRLRNIPWGVSESAYFAFDINLNYQYKAFGVPELGLKRGLVNDMVVAPYAS
                     ILALTLDPS"
BASE COUNT         1126 a          797 c          594 g         1297 t
ORIGIN      
        1 gatgggtcaa gggtcagagc aagtattgat gcatatggtg caactaccat atcattaact
       61 aatcctcgct ttagacccag ttcaggcact ccaaaggctt tatactggta atttaaatta
      121 atatcaaatg caaaatatgc tgactcggaa acgccccagg gtatattacg cagtctgccg
      181 tacctttttt gagcagcaac tacaaacctg taggtttcat ccaggagagt gttttcataa
      241 tttctgataa tcaggagagg catgaaatat tcaaacatgg tgcctgtcca ggaaaccaat
      301 cctctgtatt catctaccat ggtaagtctt cttccctgcc ggaaccagtg ctttacgtcc
      361 acttctcccc ttgcaacagc aatatagctt gtttgacgtg cttctgatgc aaaaagatca
      421 tagtaggatt tactaaacct gtttttttca ccatcgtacc ctatataaaa gagctgcctt
      481 ttttcatcaa ataaaggtgc aaattttgtg ttttcaatta acaaattgat cctttgtatc
      541 aagcacctgc ataaataagt catggcatta tcatcgttga cgacattacc attgctggct
      601 gcattatcat tatttatggc atcatcatta tttatagcat tatcattatt tatagcatta
      661 tcattattta tagcattatc attagcagct atattatcat ttttatctgt actttgtgca
      721 tgaaccctca ttgcatcaag ttctttcata aaacttgtag tcattcggta taactttttt
      781 atccatgagt atctctttag cttgctgtca gcttttattg cttccaattc tgaagcaatc
      841 atgttcaaaa tcttttccca ttctttaagg tccatattat cagttgaatg gtatttctca
      901 atataataat caattaattc ttcataaagc ttaatattgg gcatattgac ttcttctttt
      961 acaattgaaa aagtatcttt tagaccatat gccatattaa tatttacaca tgggttttct
     1021 gtttccttat ttcgtaataa ttcttttaaa gcttccgaag ccactattaa atagcccaca
     1081 aagttaccac tgtctacagc tgaaacataa gcaggccgta acaccttcag tgtagtggta
     1141 ttataccagt tgtataaatg gccttcccac ttttccattt tttccagcgt agttataata
     1201 ttatctaata actggagcat atcatagcaa ctgatatacc ccaggtctct tgcagcaata
     1261 gtagaagtca ctaataaacc aatattcgta ggggatgttc tgtgagcaac tcctttatat
     1321 ggctcttcct gtaaattatc aggtggtaaa taattgtctt ttgctgttac tgtatcctca
     1381 aaaaatcccc aggtttttct tgcagttttt cttaaaagca gaatttctgc atctttcaat
     1441 ttttctgctt ttgtttcata gggcttgctt aaaatatacg ccaaataaaa agatgaagcc
     1501 cataatacag ccaaaacaaa agatgggatt accttattca tatttatatt catatctata
     1561 gcaagagaac ctaaaagcaa tattaccccc gccaatggag ccatccacat ccttcggaaa
     1621 aaacttactg tatcattttt aagagaagct tccatatctg ctgcggttac ccactccaac
     1681 atattccttt tagtaaaaaa taacctgaat atggttctga ctatggcatc aatcatgagg
     1741 tacgcctggt atggtataaa tataagctgc agtattgtct gatataatcc agcctttaaa
     1801 ggacttatac ctgtagcata ttttccctgt ctgtacttaa aaaagtctcc ggctaaaagt
     1861 gttcttataa attgaattaa tgtataaata cctgaagcta aaactgcaag tcctacccag
     1921 atacaagcac tgccgggcaa aatcagtaat cccaaaaaca ggagcagcag taatgaagga
     1981 ttcataaggc tcctgcgcat gttgtccact attttccatt tgcaaaccgt ggaaattgga
     2041 ttgtttacta ttacaccttc tttatttctc actctggaag acaaccatgg cagcagctgc
     2101 caatcacctc ttacccacct gtgcaacctc atcgaaaaag aattgtatct ggcaggatat
     2161 ccatcaatta aatccacatc agtaacaagt cctgccctta tatagcagcc ttcaagaagg
     2221 tcatggctta atatggcgtt ctcaggtatt gcatttttaa gaactgtctg aaagatatta
     2281 aggtcatata tccctttgcc ggtgaaaata ccttctccaa ataagtcctg atatacatcg
     2341 gaaactgctg tagtataaag atctattccc ccctgtcctg caaaaattct ggtaaatgga
     2401 gaaatactgc agcttaaaat acttatgttt attctcggct gaagaatgcc atatccctca
     2461 gttactatgc ctttatcctg atctataact gctttattca ttgggtgcgc cagagtacca
     2521 attagttttt tcgctgctcc tataggcaat ttagtatctg catcaagagt tataatatat
     2581 tttacagaag gaatttcatc ctgttcacag cttaataccg tataacttgt atcgggtgac
     2641 ccctgtaaaa gatcatttaa ttcaactatt gctcctcttt tacgttccca ccccatccat
     2701 ctgttctgga cagaattatg cttccttttt cggtggaagt aaaagaatat tttttttcct
     2761 tctggtgcat actgctcatt taatctttct accccatcaa gtgctttatt gattattttt
     2821 ttgtcttcaa gagcgatctc tagatttccg tccttaaaat cgcctaccag agcaaaataa
     2881 aggttatttt ccttattaga tagataatgc acctcaagtt gatccacaag ttctattact
     2941 cttttctcat tgggtaataa ggtaggtact ataaccatgg tactcaattc ttcagaaata
     3001 ccatctttta attgcaattt aggcaaaagt gaaggccgta atattttgct tacaagagta
     3061 tttacaagag cgacagctat ttcacttgcc ggtattaata cagcaatacc cgctgctaca
     3121 gcagttgcta caagcctcgt attcgttttg atgccttcat aaacggcata atatatgaac
     3181 aatccggtaa taaccagagt aataaaagct attgagccaa tatagaaagc ctctggcttc
     3241 atttttataa tttcgtcaaa gtacctgaaa ggagcaagtt tatgctttaa ttttaataga
     3301 aggtttttcc tgccttttcc tattatataa taacctatat gcctgtattg cggtatatcc
     3361 tgttcttttg cacattcaag cgctttcaat gctataacgg tctcagaaac attgcacagc
     3421 tctgcaagtt ttcctatagc ctgtcggtaa taatttcttg attcaaaatc catttcgata
     3481 tatatgccgt ctggatcctg tttcagtatt tgttctacat ggctgagaga ttcaaatatt
     3541 tcactccagt ccatagaaga tataaactta aggcttgtca gagaattacc tatagatacc
     3601 tgggccgcag cctggacctg gtgctcaaag gatgctactt catcagattt taagccttga
     3661 tcatgcagtt tttcattaac atatataaga gcagccgttc ccttcctccc ctgttttttc
     3721 aatttattaa gcaaatgctc aataaaggag gaatatactg cacctctttc tcttacatac
     3781 tgatttatta tatataatac ttcttctttg ccct
//
LOCUS       DAOSDD010000345         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8616_length_3761_cov_6.191042, whole genome shotgun sequence.
ACCESSION   DAOSDD010000345 DAOSDD010000000
VERSION     DAOSDD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8616_length_3761_cov_6.191042"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1716
                     /locus_tag="PK733_18720"
     CDS             73..1716
                     /locus_tag="PK733_18720"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12603.1"
                     /translation="MKKFLCIMLSVMLVLSIVTGCSSSKDKDKDNTSAAVEKEDTKQG
                     EIFNKTGYPIVNKPVEVTMMHSKGSHQGPWEEMHYFKAMEKATNIKIKFLSIESSGYE
                     EKKNLAFASGDIPDVVWGLTSKEEVKYGVEGKTLIPLQNYINNYAPNYAKMIKESPDV
                     KAVATSMDGNMYSFPVLANTLTMATNYLYIRTDWLDKVGKKIPETVDEYIDVCAAIKA
                     WNNSKIPVSCAKESFSNLVRAFLPAFGYLVDDRYGLDETDKVVYTPITEQYKLVLMFL
                     NKLYANKYLDNECFTQEDANVGAKMKENISMMSDWGSVMKPENFENGVIQMAMLAPLT
                     SEYYDKKHVRGFDTIFSSQIALTKNLKYPEAMARWIDINYSDEDVAPGIVKGLNRISI
                     WLGIEGQDWQYTDNEKTTYARIVPPDIKASEIEYAEKYVSPGWGPCKLVMLAMPEKEK
                     NPGQYMKASQSKANYFPYMEPGFKNNYLKYTPEEQERLKILENDIDSYTKQMEAKFVT
                     GQEKIENWDKYVQKLKEMGVDELIKIKQDAYGRYKLLVK"
     gene            2095..3033
                     /locus_tag="PK733_18725"
     CDS             2095..3033
                     /locus_tag="PK733_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ12604.1"
                     /translation="MARFVKISSLCPAPYIVDPQLDYSIAVDEMINHWNNQFSQVLPD
                     KPDLIITHEACDRSPVYSLESRLEYYRVRGNKVRDFMRKVAAENNCYIAYSAAREMLD
                     GTWRNSTQIIDRNGNIAGIYDKNHLVIEENTEAGIAYGKDAPIIKCDFGTIGCAICFD
                     LNFEELRLKYAKARPDLIVFSSMYHGGLMQNYWAYSCRAHFAGSIAGLQGTIISPVGE
                     TIAKTTNYFKYITVDVNLDCEVIHLDWNWDKIRAAKEKYGSKFKMSDPGYLGAVLISS
                     ESDEVTINDIIKEFDFELLDDYFNRSLAHRHENLVP"
     gene            3098..3613
                     /locus_tag="PK733_18730"
     CDS             3098..3613
                     /locus_tag="PK733_18730"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ12605.1"
                     /translation="MDVMAAIQGRRSIRNYSTREVEEEKLNRVLEAARLAPSANNRQN
                     WRFIVVRDEELRKKMTEASFGQQFVGGAPVVIVACGTDPSGVMRCGQYRYTIDLSIAV
                     SYMTLEAYEQGLGTCWIGSFDEGMVKKLLNIPDTVRAVAITPLGYPAESPAPRPRKSL
                     SEIVCYDKYQE"
BASE COUNT         1312 a          551 c          821 g         1077 t
ORIGIN      
        1 tctaacaaaa aataccgtag tttcggggct tttagccccg tgaccacgag ttttacaagg
       61 gaggtaatga aaatgaaaaa gtttttatgt attatgctaa gtgttatgct ggtgttaagc
      121 atagttacag ggtgtagcag tagtaaggat aaggacaaag ataatacgtc tgctgctgtt
      181 gaaaaagaag atactaaaca gggtgagata tttaacaaaa caggttatcc tatagtgaat
      241 aaacctgttg aagtaactat gatgcattca aaaggctcac accagggacc atgggaggaa
      301 atgcattatt ttaaagcaat ggagaaggct actaatataa aaattaagtt tttatcaatt
      361 gaaagttcag gctatgaaga gaaaaagaat cttgcctttg caagcggcga tattcctgat
      421 gtagtatggg gacttacatc aaaggaggaa gttaaatatg gtgtggaagg taaaactctt
      481 attcctttac agaattatat aaacaattat gcacctaatt atgcaaaaat gataaaagag
      541 tctcctgatg ttaaagctgt agctacttca atggatggga atatgtactc ttttcctgta
      601 cttgcaaata ctctgacaat ggctaccaac tacttataca tcaggacaga ctggcttgat
      661 aaagtaggga agaaaattcc tgaaacagta gatgagtata tcgatgtctg tgcagcaatt
      721 aaagcatgga acaactctaa aatacctgtt tcatgtgcta aagagagttt ttccaatctt
      781 gtacgtgcat ttttacctgc atttggttat ttagttgatg atagatatgg attagatgaa
      841 actgacaaag ttgtttacac accaattact gagcaatata aattagtact tatgttttta
      901 aacaaactgt atgcaaataa gtatctggat aatgagtgtt tcacacagga ggatgcaaat
      961 gttggcgcca agatgaaaga aaacataagt atgatgtcag attggggttc agttatgaaa
     1021 ccagaaaatt tcgaaaatgg agtaatacag atggcaatgc ttgctccatt aacttccgag
     1081 tattatgata aaaagcatgt taggggattt gatacgattt ttagctcaca aatagcactt
     1141 acaaagaatt taaagtatcc ggaagctatg gcaagatgga ttgatataaa ttattctgat
     1201 gaagatgtag ctcctggcat tgtaaaagga ttgaatcgta tctcaatatg gctgggaatt
     1261 gaaggacaag attggcaata tacagacaat gagaaaacta cttatgcaag gattgtacct
     1321 cctgatatta aagcgtctga gattgagtat gcagaaaaat atgttagccc gggatggggt
     1381 ccgtgtaaat tggtaatgct tgcaatgccg gaaaaagaaa agaatcccgg ccaatatatg
     1441 aaagcatccc aatcaaaagc aaactatttc ccatacatgg aaccaggttt taagaataat
     1501 tacttaaagt atacacctga agagcaggaa agattaaaaa tactcgaaaa tgatattgac
     1561 tcatatacaa aacaaatgga agccaagttt gtaacaggac aggaaaagat tgaaaattgg
     1621 gataagtatg ttcaaaagct taaagaaatg ggagttgacg agcttataaa aatcaaacag
     1681 gatgcatatg gtagatataa attattagta aagtaagcaa agcaggttga gctaattata
     1741 tatgttaagg actagtatag tgtttctcaa atacctatac aaatgaaaaa cctgcgaacc
     1801 cgtaaaatca tcgggattct cggcttttca gaaaatgagg taatttgaga acacactact
     1861 atataattta cgatttgaaa gaactaatga agaatttgca aatcgtaagt tacatcaagg
     1921 taacagaagc aggttaaatg ttttaaaacc tgttctatat agtaataaaa tacctgatta
     1981 tagcaggagg ccggattaat tgtagttagg cctcctgttt tgctactata gatatagcaa
     2041 cctttggaaa taggtgatat aattaagttt aagaaaatga aaggggatat gaatatggca
     2101 cgttttgtaa aaatcagttc actgtgtccg gctccgtaca tagttgaccc tcaactggac
     2161 tatagtatcg cagttgatga aatgatcaac cattggaata atcagttttc acaggttctt
     2221 cccgataagc cggatttgat tattacccat gaggcttgtg accgttcacc tgtttattca
     2281 ctggaaagta gacttgaata ttacagagtt cgaggaaata aagtaagaga ttttatgaga
     2341 aaggttgcag ctgagaataa ttgctacatt gcttattctg ctgcaaggga aatgcttgac
     2401 ggcacatgga gaaattcaac acagattata gacaggaatg gaaatatagc aggtatttat
     2461 gacaaaaatc accttgttat agaggaaaat actgaagcgg gtattgctta tggcaaggac
     2521 gccccaataa taaaatgtga ttttggaaca ataggttgtg ctatatgttt tgacctcaac
     2581 tttgaagaac tgagactaaa atatgcaaaa gcccggcctg atcttatagt tttctcctca
     2641 atgtatcatg gagggttaat gcaaaattac tgggcatatt catgcagagc ccatttcgcc
     2701 gggtctattg ccggattgca gggtaccata atatcacctg taggagaaac aattgcaaaa
     2761 actacaaatt attttaaata tattacagtt gatgttaacc ttgattgtga agttatacat
     2821 ttagattgga actgggataa aataagggct gcaaaagaga agtatggaag caagttcaaa
     2881 atgtccgatc caggatatct gggagcagta ttaatatcta gtgaatcaga tgaagttaca
     2941 ataaatgata ttattaaaga atttgacttt gaactgcttg atgactattt taacagatct
     3001 ttagctcaca ggcatgagaa tcttgtgcca tgactgggaa acattcataa ctttagtcat
     3061 aagaaagttc aaaaaaggag aggaaaggtg attttttatg gatgttatgg cggcaattca
     3121 aggaagaagg agcataagaa attatagtac cagggaagtt gaagaggaaa aactcaatag
     3181 ggttttggaa gcggccagac ttgctccttc tgcaaacaat aggcagaact ggaggttcat
     3241 agtagtcaga gatgaggaac tgcggaaaaa gatgacagaa gcatcatttg gtcagcagtt
     3301 tgtaggtggt gctccggttg taattgttgc atgcggtact gatccttcag gagttatgag
     3361 gtgtggccag tacaggtata ctattgattt atccatagct gtatcatata tgacgctgga
     3421 ggcctatgaa cagggtcttg gcacatgttg gataggcagt tttgatgaag gtatggtaaa
     3481 aaaattactt aatatacctg atacggtgag agctgttgct ataactccat tggggtaccc
     3541 tgctgaaagt cctgctccta gaccaagaaa atcgttaagt gagattgtat gctatgataa
     3601 gtatcaggaa tgatttgtgt taatgatgtt ctgtaaaaaa agtgcagtga atttaatatt
     3661 ttaaaatcac tgcacttttt gtttcttgga gctggtggac ggaattgaac ccccgacctg
     3721 ctgattacaa gtcagctgct ctacctactg agctacacca g
//
LOCUS       DAOSDD010000346         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8697_length_3731_cov_5.044342, whole genome shotgun sequence.
ACCESSION   DAOSDD010000346 DAOSDD010000000
VERSION     DAOSDD010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8697_length_3731_cov_5.044342"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK733_18735"
     CDS             <1..317
                     /locus_tag="PK733_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12606.1"
                     /translation="IDSIDPLARMTQKTAYRRDEIMAVLEKAADWVLQNRIEDGGFVF
                     ILDRPFEYGHRELYGEKSKGAMFPTWFRTLSLAVIGKAVPENPLGKYPWNFIMHPGFQ
                     FW"
     gene            433..2637
                     /locus_tag="PK733_18740"
     CDS             433..2637
                     /locus_tag="PK733_18740"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ12607.1"
                     /translation="MNKEKFASLFPIGSHLCREPMPSMSEMKKDMEILKKNGFNLIKL
                     QENWAIDEPLEGYYDFSRYEELIEYAVELDMGVYLGLTCEQAPNWLWQKYPDCRMVGR
                     NGVPVAYQAQTTLPADGKPGPCYDHPGAMSDQLRFIRKLVEVLGKYENIVVWNTWQEI
                     AYWAEGLVGQHICYCENTLEHFRRWLMEKYGDLDNLNRTWKSRYMDWSYVLPDRLVGR
                     TCLPNDIDWKYFMDNIQIAHVLKSREKAIKDTDPLKRPVFAHKGAPVIGSGADWTYAR
                     TQDFLGSSCYPAWGSGHGWDDVKQAPLIGREDALLAEMWDGVALKYDYIRSCNKKGAP
                     IWAAEFQGGPVSTGFQYGRIPSRDEIRRWMLTAVGAGVTAISFWVTRAEIMAAEMNGF
                     SLLDSEGDTTERIEEAGRVGEALNRHADLFANPSWGGASIAILVNEWNYQLCMSLAQG
                     GENLPYSLRGWYRILWDASIPVDFIEASELNEDYIQEYNAIILPFPLSISEEIACKLK
                     KYVQKGGNLISEACPGRLNDYGFANRGELSPVLRELFGVTQKSFKMVREPNGGKRWSP
                     QERTWGEYLEAAMLEGMGAFEGMRLRANIYIETFECKQDSVPFLKYKDAIVGTVRKVD
                     DGNAWLIGTFARHNGTAYRDSESRAFVLALLKECGIIPAYEGKLLLRKRIRGDKEAWL
                     FTNPTGEDITESIDVRNIKHVEDLMGEELNIKDGFISLTVKGLDVRALVVNK"
     gene            complement(2710..3726)
                     /locus_tag="PK733_18745"
     CDS             complement(2710..3726)
                     /locus_tag="PK733_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ12608.1"
                     /translation="MEKLTIKEIAKLAGVSPTAVSFVLNNKKGVSDKTRKKVNEVIEK
                     TNFIPSLNSRRLFFKKSYNISLVVQQTSSPFSDLFYYEITNGILEKNKEFGYNIVFTD
                     ISMKGNSMDIPNIIKYKDTDGIIFYQDTERAILREVDKLNIPYVIVDAHSTSSDFTTI
                     NADSELLSYAATKFLIEHGHVDIALISSSYIPEYYIQIFTGYKNALEEVRVSISPYWI
                     QNNAFDELSAYNCMENILKNPSTPSAVFCAGDIFAIGAIRCAKNKGYRVPEDISFIGI
                     DDIILSRYIEPALTTIRIDKALMGSLAMDLLVKKINGESVESIILESDNIVVRDSVKN
                     INCN"
BASE COUNT         1140 a          627 c          859 g         1105 t
ORIGIN      
        1 acattgattc aatagatcct cttgcgagaa tgacgcaaaa gactgcatac cgaagggatg
       61 aaatcatggc agtactggaa aaagcggctg attgggtgtt gcagaaccgg atagaggacg
      121 gaggttttgt ttttatactg gatcgccctt ttgaatatgg acacagggaa ttgtatggag
      181 aaaaaagcaa aggagcaatg ttccccacct ggtttaggac actatcgctt gcagttattg
      241 gtaaagcagt tcctgaaaat cctcttggaa aatatccgtg gaattttatt atgcacccgg
      301 gatttcagtt ctggtgattt aagtatgctg attcagcact ctattcaagg agggtttatg
      361 tgtgaatgat ggatttgaaa agatatttaa aaatgatgtt ttatcatatt tagctttaac
      421 ggaggattat ctatgaataa ggaaaaattc gcatcattat ttcctatagg ttcacacttg
      481 tgccgggaac ctatgccttc tatgtctgaa atgaaaaaag acatggaaat attgaaaaaa
      541 aacggcttca atctaataaa gctccaggaa aattgggcaa ttgacgagcc actggaagga
      601 tattatgact tttcaagata cgaagaattg attgagtatg ctgtagaact agatatggga
      661 gtttacctgg gacttacctg cgaacaggct ccaaactggc tatggcagaa atatcctgat
      721 tgtaggatgg tgggcaggaa tggagtacct gttgcttatc aagcacagac taccttacca
      781 gctgatggaa agcccggacc atgttatgac cacccgggag ctatgtcaga tcagttacgg
      841 tttatcagaa aactggtgga ggtgcttgga aaatacgaaa atattgtagt ttggaataca
      901 tggcaggaga tcgcttattg ggcagaaggt ctggttggcc agcatatatg ctattgtgaa
      961 aacaccctgg agcatttcag aagatggctc atggaaaaat atggggatct ggacaaccta
     1021 aaccgtacct ggaaaagcag gtatatggat tggagctatg tgcttcccga taggcttgtt
     1081 ggaagaactt gtcttcctaa cgatatcgac tggaaatatt ttatggacaa tatccagatt
     1141 gcacatgtgc taaaatcaag ggaaaaagca attaaagata ctgatcccct taaacgtccc
     1201 gtttttgcac ataaaggagc tccggtgatt ggttcaggtg ctgactggac atatgcccgt
     1261 acacaggatt ttttgggttc ttcctgttat cctgcatggg gttcgggaca tggttgggat
     1321 gatgtaaaac aggcaccttt aatcggtaga gaagatgcac ttttggctga aatgtgggat
     1381 ggtgttgctt tgaaatatga ttatatccgc tcatgcaata aaaaaggtgc accgatatgg
     1441 gccgcggagt ttcaaggagg acctgtgagt acaggcttcc aatatgggag gataccgtcc
     1501 agggatgaaa tacggagatg gatgttaaca gctgtaggtg caggtgtgac agccatttcc
     1561 ttctgggtta cccgtgctga aataatggca gctgaaatga atggatttag ccttctggac
     1621 agtgaaggag atacaacgga acgtatagag gaagccggac gagtaggaga agcgctgaac
     1681 agacatgcgg atttgtttgc caaccccagt tggggaggtg ctagcatagc tatcttggtc
     1741 aatgaatgga actatcagct ttgcatgtct ctagctcagg gaggtgaaaa tctgccttac
     1801 agcctgcggg gttggtacag gatactatgg gatgccagca taccagtaga ctttatagaa
     1861 gcttcggagt tgaatgaaga ttatatacaa gaatacaatg ctattattct accgttccct
     1921 ctgtccattt cagaggaaat tgcatgtaag ctgaagaaat atgtgcagaa gggcggaaat
     1981 cttataagtg aggcatgtcc gggaagactt aatgattacg gttttgccaa ccgaggtgaa
     2041 ttatcaccgg ttttacggga gctttttggt gtgactcaaa agagcttcaa aatggtcagg
     2101 gaacctaatg gtggcaaacg atggtctcca caagaacgta catggggtga atacttggaa
     2161 gcagccatgt tggaaggaat gggagccttt gaaggcatga ggttacgtgc taacatatat
     2221 attgaaactt ttgagtgtaa gcaggacagt gtaccctttc ttaagtacaa agatgcaatt
     2281 gtaggaactg taaggaaggt agacgatggt aatgcgtggc ttataggcac ttttgcaagg
     2341 cacaacggta ctgcttacag agattcggag agtagagctt ttgtactggc tctactgaaa
     2401 gaatgtggta ttatacctgc atatgaaggt aagcttctac ttcgcaaaag aattaggggc
     2461 gataaggagg catggctgtt tacaaatcct actggggaag atattacgga gtcaatcgat
     2521 gtcagaaaca taaaacatgt ggaagatctt atgggtgaag agcttaatat caaggatggt
     2581 ttcattagct tgacggttaa agggcttgat gtaagggcgc ttgttgtgaa taaataatat
     2641 tacttgtaca ttggttattt cgaaatgata gagatgacat taactgaaga taatgtcgtc
     2701 tcttcagttt taattacaat ttatattctt tactgaatcc ctaacgacaa tattatccga
     2761 ttccagaatg atgctttcta cactttcacc atttatcttc ttgaccaata aatccattgc
     2821 aaggctcccc atgagagcct tgtctattct aatagttgta agagccggct ctatatacct
     2881 ggataaaatg atatcatcta ttccaatgaa agaaatatct tcaggaaccc tatagccttt
     2941 gttctttgca catctaattg caccaatagc aaaaatgtca ccagcacaga agaccgcaga
     3001 aggtgttgat ggatttttca aaatattttc catacaatta taggcagata attcatcaaa
     3061 tgcattattc tgaatccagt agggagatat ggagacacga acttcttcca gagcattttt
     3121 gtatccggta aatatttgaa tgtagtattc agggatataa ctggaactaa taagagcgat
     3181 atcaacatgc ccatgttcaa taagaaattt tgttgcggca tatgatagaa gctcactgtc
     3241 agcatttatt gttgtaaaat cgctgcttgt tgaatgtgca tctacgatta cataaggtat
     3301 atttaattta tcgacttccc ttaatatggc tctttcagta tcttggtaga atataattcc
     3361 atccgtatct ttatacttga taatattcgg aatatccatt gagtttcctt tcatagagat
     3421 gtctgtaaaa acaatgttat aaccgaactc tttgtttttt tcaagaatcc catttgttat
     3481 ttcataataa aaaagatcag agaatggaga agaagtttgc tgtaccacca gacttatatt
     3541 atagcttttc ttaaaaaaaa gccttcgtga attaagactg ggtataaaat ttgtcttttc
     3601 aattacttca ttaacttttt ttcttgtttt atcacttaca ccttttttat tatttaaaac
     3661 aaaggacaca gcagtaggtg aaacacctgc taattttgca atttctttaa tagttagttt
     3721 ttccatctaa t
//
LOCUS       DAOSDD010000347         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8786_length_3705_cov_3.205479, whole genome shotgun sequence.
ACCESSION   DAOSDD010000347 DAOSDD010000000
VERSION     DAOSDD010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8786_length_3705_cov_3.205479"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PK733_18750"
     CDS             complement(<1..702)
                     /locus_tag="PK733_18750"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12609.1"
                     /translation="MNNNYKNDTNTFEAIRLHLELKGEYFDDEEKRILNKYGKSSTGD
                     SISRDILIPSDMPLHNLHYAIQRLFGWQNSHLRRFILPEEIYNRLTGSTVKGWADLVG
                     ILFQPPSEHEHDVFWDDDYKKGSIKTWLRKKYTGPYSFEGIMEDYERAQNDIKQLIES
                     FPMLEVHESFNDYYNRSKGKDNVKKRILKKAPLIELTLEEMNNSIYMEGGTENLLERL
                     EVSKVLAFNDENVINN"
     gene            complement(790..1602)
                     /locus_tag="PK733_18755"
     CDS             complement(790..1602)
                     /locus_tag="PK733_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ12610.1"
                     /translation="MNVIITGASGGLGRVLATECGRRGYNIFLTDINQRGLISLKQGI
                     ERQFKVCVTAKACDLTNADSVDQLMSVIDYHGICFDMLINVVGIDYDGDFLGQDREKI
                     TKIVSINNEATLRITHDILRRRRTGKKFTLIFVSSLASMYPMPLKATYAASKRFLLDF
                     ALALSQELKNKNANVLTLCPGGLVTTKEAMRGIAVQGFWGSVTTNPLEVVARKTIDNA
                     LNGKRIYIPGLFNRVLSFFGKIIPRSWVAAVIYFRWNKAQNKWMQYGEPKST"
     gene            complement(1607..2458)
                     /locus_tag="PK733_18760"
     CDS             complement(1607..2458)
                     /locus_tag="PK733_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ12611.1"
                     /translation="MKTVVITGASSGIGFETARLLSKNYSVIGIGHNKSNCERAKGLI
                     KSENPNADVIYFVADLIQQNEVNRVADEVASYIESFRESELYGLINNAGCVRSWYTTS
                     EEGYEQQFSLNHLAGFLLTYRLLPFIIKAKGRIIMTGSQSHKGIKMRWDDVMLRKRYN
                     PLTAYKQSKLCNILFAKGLNDKFFNTGIRAYVVDPGLVKTDIGNKYTGGLVSFIWSIR
                     KKGGVSPEVPAKTYEFLLNRQPAPTGLYYNQCKEKEYSRQVTDKNADRLFMLSQELCG
                     ISYDRQD"
     gene            complement(2458..3174)
                     /locus_tag="PK733_18765"
     CDS             complement(2458..3174)
                     /locus_tag="PK733_18765"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HOJ12612.1"
                     /translation="MTKSEFLTQLRYELKKNNISDSEDILDEYEQHFAFKLADGFAEE
                     EIAAKLGNPYELASQFIPNEEQKKHGGRKIVTVIGMVFADIFVGAFFLILYAFAAVMG
                     TFTIAAAFIGICLLTGLNISNLIPGMPYLCALIYVVCLLALALLSSVGCLYFVSFIKQ
                     LMRSYGRFHFNVIAGSAGQAMLPSLPVYPRMSAKSKRRTRLIALFSLTVFAVSFIAGY
                     IISAILAGSLEFWHVWGWFI"
     gene            complement(3176..3487)
                     /locus_tag="PK733_18770"
     CDS             complement(3176..3487)
                     /locus_tag="PK733_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ12613.1"
                     /translation="MNTQYKKGVLELCVMSLLKRHDWYGYEISEYLSRHIDIADGTVY
                     PILRKLKSDGFVTTYLSEDSGGPPRKYYSLTELGLETYEAAKQEYINFAKSIETILED
                     E"
BASE COUNT         1119 a          800 c          590 g         1196 t
ORIGIN      
        1 attattaata acattttcat cattaaaagc taaaaccttt gacacttcaa gccgctcaag
       61 cagattctca gttccgcctt ccatatatat ggaattgttc atttcctcta aagttaattc
      121 aataagcggt gcttttttta aaattctttt ttttacattg tctttcccct tactcctatt
      181 ataatagtca ttaaaagatt catgaacctc aagcattgga aaactctcaa ttagttgttt
      241 aatatcattt tgagcccgtt cataatcttc cataattccc tcgaaagaat atggtcctgt
      301 atatttcttt ctaagccatg tttttatact cccttttttg tagtcatcat cccaaaatac
      361 atcatgctcg tgttcgctgg gaggctgaaa aagtatcccc accaaatcag cccagccttt
      421 gactgtactg ccagttaacc tgttatatat ttcctcaggt aggataaatc tccgcaaatg
      481 agaattctgc catccaaata atctttgtat agcataatgt aaattgtgca ggggcatatc
      541 ggatgggatc aaaatgtccc tgctgattga atcacctgtt gatgattttc catacttgtt
      601 aagaattctt ttttcttcat catcaaaata ttcacctttc agttccagat gaagacgtat
      661 tgcttcaaac gtatttgtat cattcttata attattgttc atatgttttt cctctccaat
      721 cagtctcata ctctcctaat gtgattagcc tgttgtctta tatgttttta atggaattgt
      781 tagtttatac taagttgact ttggttctcc atattgcatc cacttattct gtgctttatt
      841 ccatctaaaa tatatcacag cagccaccca tgaacgagga ataatctttc caaagaatga
      901 aagaacgcgg ttaaacagac cgggaatata tatcctttta ccattgagtg cattgtcgat
      961 cgtcttgcgt gcaacaacct ccagcgggtt tgttgtgaca ctgccccaga aaccctgtac
     1021 cgcaattccg cgcatagctt cttttgtggt aaccaaaccg cctggacata aagtcagaac
     1081 atttgcattc ttgtttttaa gctcctggct taaggctaat gcaaagtcga gtagaaatct
     1141 ttttgatgcg gcatacgttg ctttcagtgg catgggatac attgaagcaa gacttgatac
     1201 aaaaatcagc gtgaactttt taccggttct tcttcttcgt aaaatatcat gagttatcct
     1261 taaggtcgct tcattattaa ttgaaacaat cttcgtgatc ttctcccgat cctgtcccaa
     1321 aaagtctcca tcataatcta taccgacaac attaataagc atatcaaagc aaataccgtg
     1381 ataatctata acagacatca gctgatcaac gctatcagcg ttggtaagat cacaggcttt
     1441 tgctgttaca catactttaa attgtctttc aatgccttgt ttcaatgata tcaatcctct
     1501 ttggtttata tcggttaaaa aaatattgta gcctcttctg ccgcattccg tggcaagtac
     1561 acggccaagg ccaccgctgg cacctgttat tataacattc atatctttaa tcctgcctgt
     1621 catatgatat tccacacagc tcctggctaa gcataaataa tcggtctgca tttttgtcag
     1681 ttacctgtct gctgtattct ttttccttac actggttata gtaaagaccg gtaggggcag
     1741 gctgcctgtt aagcagaaat tcataagttt tagctggaac ttccggtgaa acaccaccct
     1801 ttttcctgat tgaccatata aaacttacaa gacctccggt atatttgttt ccaatatcgg
     1861 ttttaacaag tccggggtct acaacataag ctctgatacc tgtattaaaa aatttgtcat
     1921 taagaccctt tgcaaacaaa atattgcata attttgactg cttataagct gtcaaaggat
     1981 tgtatctttt ccttagcatc acatcatccc aacgcatctt tataccctta tgagattggc
     2041 ttccagtcat aattattctt ccctttgctt tgattataaa cggaagaagc ctatatgtca
     2101 gcaaaaatcc tgcaagatgg tttaaagaaa actgttgctc atatccttct tcggaagtgg
     2161 tgtaccagct tcttacacaa cctgcgttat taatgagacc ataaagctca ctttctctga
     2221 agctttcaat gtaagatgcc acctcatcag caacacggtt tacttcattt tgctgaataa
     2281 gatccgcaac aaaataaatt acgtctgcat tcgggttttc tgactttatc aggccttttg
     2341 cacgctcaca attgcttttg ttatgtccta tcccaatcac agagtaattt ttagataaaa
     2401 gacgtgcagt ttcaaatccg attcccgaag atgcacctgt aataacaact gttttcatta
     2461 tataaaccat ccccaaacat gccaaaactc aagacttcct gctaaaattg ccgatattat
     2521 ataccctgca ataaaactaa ccgcaaaaac agtaagagaa aaaagtgcaa tcaaccttgt
     2581 acgccgtttg gatttggctg acatccgggg atataccggc aatgaaggaa gcattgcctg
     2641 ccctgcggag cctgctataa cattaaaatg aaatcgtccg taagaacgca taagttgttt
     2701 gataaaactt acaaaataca aacatccaac gctcgacaat aaagctaaag ctaacagaca
     2761 aacaacatat ataagagcgc ataaatatgg cataccggga attaaattac taatattgag
     2821 tcctgttaac agacatatac ctatgaaagc tgctgcaata gtaaaagtac ccatcactgc
     2881 ggcaaaagca tagagaatca aaaagaaagc accaacaaag atgtctgcaa aaaccatgcc
     2941 aataactgtt acaattttcc tgccaccatg tttcttttgc tcttcattgg gaataaactg
     3001 tgaagccagt tcataagggt ttccgagttt tgctgcaatt tcctcttctg caaaaccatc
     3061 ggcaagctta aatgcaaaat gctgctcata ttcatccaga atatcctccg agtcagaaat
     3121 attgttcttc ttcaattcat atcttaactg tgttaaaaac tcacttttcg tcatcttatt
     3181 catcctccaa tatggtttcg atggattttg caaaattaat gtactcctgt ttggcggctt
     3241 catatgtctc aagccccaat tcagtaagag agtaatactt tctgggtgga cctccgctat
     3301 cttctgaaag gtatgtggta acaaatccat cacttttaag ctttcttaaa attggataaa
     3361 ctgtaccgtc tgcaatatct atgtgtcttg agagatattc cgatatctca tatccatacc
     3421 aatcatgacg cttaagaagc gacatcacac aaagctccaa tacgcctttt ttatactgtg
     3481 tattcaagtt aacctcctaa acagatacct gtaatgtaca ctgtctacta ctatattata
     3541 ttcaatagtg atgtatatgt caatacctga atcagtttct tttgttctta ttaaaacgtt
     3601 actattcaga ggtaactgag cagatatagg tatgcaatat aaggaaacat tagcgaaatg
     3661 cagcttagag atgtatagtc agagcgagga atagcaagcc gactg
//
LOCUS       DAOSDD010000348         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8835_length_3688_cov_3.507294, whole genome shotgun sequence.
ACCESSION   DAOSDD010000348 DAOSDD010000000
VERSION     DAOSDD010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8835_length_3688_cov_3.507294"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PK733_18775"
     CDS             <1..654
                     /locus_tag="PK733_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12614.1"
                     /translation="GMRVRRFTGLLKEVELDTKGPLFARVELKYELEGTDSYSVMLTA
                     YSSIPRVDVSVRINKNNRLEPENVYIALPFTTGQGQKPQLWIEKTGHILRPRVDQLPG
                     TGVDFYCVQEGVTYSSEEKGLVIAMPDTPMIQLGPLEFGERQLMGYNGIKEDGEHLYS
                     WVMNNYWETNFKGTIGGFYEFRFSVNWGKELGNPETAITRCHSINTGLIGIRVTDRI"
     gene            1019..1297
                     /locus_tag="PK733_18780"
     CDS             1019..1297
                     /locus_tag="PK733_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12615.1"
                     /translation="MDCAKQPLEQFEAKYPYECRPRICLELCEDWARGRIKMLTAKRA
                     ILDSHAVAKEIDDKEYGAFIYSFIFIFTSNHIEFLRLLSKGKTLQSDF"
     gene            1724..2224
                     /locus_tag="PK733_18785"
     CDS             1724..2224
                     /locus_tag="PK733_18785"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ12616.1"
                     /translation="MRNIVLIGMPGAGKSTVGVLLAKTLGLSFVDIDLVIQEKEGMLL
                     QDIIDMYGISRFIQIEENAVLSLKCKAAVIATGGSVVYSDKAIMYLKQDGIIIYLKVG
                     YEEILRRINNISTRGIVLNKGQSLLDMYNQRISLYEKYSDITIDCSNKDVEDIIQIIV
                     NDVSNR"
     gene            2344..2814
                     /locus_tag="PK733_18790"
     CDS             2344..2814
                     /locus_tag="PK733_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOJ12617.1"
                     /translation="MAISKVREYLKKWDKGNDILEFETSSATVELAAQALGVDPARIA
                     KTISFKNKEGAMLIVAAGDVKTDNGKFKAEFGFKAKMLTPEETLEYTGYAVGGVCPFG
                     LKNDIPVFLDISLKRFETVFPACGSSNSAIELTCEELDKFSLNKKWVDVCKGWT"
     gene            2928..3137
                     /locus_tag="PK733_18795"
     CDS             2928..3137
                     /locus_tag="PK733_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12618.1"
                     /translation="MLSSFAIVENKSKLWLMYEDKVLIGDNFYDEFRLTRIQEKSQFN
                     ALLKLVSVNRTLGQKLHIAANRFSD"
     gene            3323..3481
                     /locus_tag="PK733_18800"
     CDS             3323..3481
                     /locus_tag="PK733_18800"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HOJ12619.1"
                     /translation="MQNAVERALNQIDDKNYADELKIAGIKEIIKVGITFCGKEVSLG
                     YRNEVFKL"
     gene            3527..3664
                     /locus_tag="PK733_18805"
     CDS             3527..3664
                     /locus_tag="PK733_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12620.1"
                     /translation="MGRLVFKTLLSKYDELIKIIGRYKKIIDTPNTDITWKKVSGRMD
                     R"
BASE COUNT         1334 a          493 c          814 g         1047 t
ORIGIN      
        1 ggtatgagag tcaggcgttt tacagggctt ctaaaagagg tcgaattgga tactaaaggg
       61 cctttattcg caagagtgga gctaaaatac gaacttgagg gtactgatag ttattcagtg
      121 atgctgaccg cctattcaag cattccacgt gttgatgtat ctgtccggat aaataagaat
      181 aacagattgg aacctgaaaa cgtatatatt gccctgccat ttacaacggg tcaaggccag
      241 aaacctcaac tatggattga gaaaaccgga catatcctga ggccaagggt agaccaactt
      301 cctgggacgg gagttgactt ttactgcgtt caggaaggcg taacctattc atcggaagaa
      361 aaaggtttgg ttattgctat gcctgataca ccgatgatac agttaggccc cctggagttt
      421 ggagagaggc agttgatggg ttataacgga ataaaggaag atggcgaaca cctgtattcc
      481 tgggttatga ataactactg ggagactaat tttaaaggta caattggagg attttatgag
      541 ttcagatttt cggtaaattg gggtaaagaa cttggtaatc ccgaaactgc tattacgaga
      601 tgccactcca ttaatacagg gcttatagga atcagagtta cagacaggat atagatgcag
      661 ctattgcaag aggtattatt gaagatagga aaaaggtaat cagtccagga gaaagcgaat
      721 gtgcaggata tgctcaggca ttgggaattc caataataat atcagataat tacacagaat
      781 ttaagttgtc agatgaattc attactttga catgcaataa cattttggct ttatgtgtgt
      841 attttgatga gatttcaaaa agtgagcaga agacattttt aataaaatta atgataagtt
      901 gacttgtccg actggggata cctttgaaga ccaatataga aaagctttaa taagattaga
      961 gaggaatggt tggaaaaaat atttgggaat atagaggaag gtatatgtaa taagggatat
     1021 ggactgcgca aaacagccct tggaacaatt tgaagcaaag tatccctatg aatgcaggcc
     1081 tagaatctgc ttagaacttt gcgaggattg ggcaagaggc agaatcaaaa tgctcacggc
     1141 aaaacgggct attttagatt cacatgcagt tgcaaaagaa atcgacgata aggaatatgg
     1201 tgcttttata tattccttta tttttatttt tacttcgaac catattgagt ttctgcggtt
     1261 gctttcgaaa ggtaaaactc tgcaatctga tttttagaat gtttacttca atacgagaag
     1321 ggtaagagta aataaataaa taattgcaag aaaataacaa gccagtgttt tcaggggttt
     1381 aaaggatgtc catgaaagga ctttatttaa ttatggaaag gagtttgttt gatgaacaca
     1441 tttagtgcct gagtcaaata aactcctatc attttgcaat atcaagactt ttgtccaaaa
     1501 ctcctagttg aaagatataa gaaaaatata taaaatataa acaatagaag tgattggggt
     1561 taattggcgg ataatgcaca aaaagcagaa gcgttttaat ataacgatac ggaaattata
     1621 aactcaaaca ggctaagcac attacacatt atatataata ctcaagaatg atgcatatga
     1681 aagagtagcg ccaaatggaa aggataaaag tcggaggttc ttcatgagaa acattgttct
     1741 tattggaatg ccaggtgcag gtaagagtac agtcggtgtt ttgcttgcta aaacattggg
     1801 attaagtttt gtagatattg atttggttat acaggaaaag gaaggaatgc ttttgcagga
     1861 tattattgac atgtatggta tcagcaggtt cattcaaatt gaagagaatg cagtgctttc
     1921 attaaaatgt aaagctgctg tcattgccac cggaggcagt gtagtatata gtgataaagc
     1981 aataatgtat ttaaaacagg atggaataat tatttattta aaggtaggat atgaggaaat
     2041 cttaagaagg attaacaaca tatcaacaag aggaattgtt ttgaataaag gtcaaagtct
     2101 tcttgatatg tataaccaaa gaatatcttt gtatgagaaa tattcggata ttactattga
     2161 ttgttcaaat aaggacgtgg aagatattat acaaattata gtaaatgacg taagcaacag
     2221 gtaagtagta aaactggcaa atacaatata ctgtttccct caaaaagcta gtaaaatagt
     2281 aactgcagca gacagcaaaa taaacgaaat ataaaacaat ttcaaatgaa aggactaatc
     2341 attatggcta ttagtaaagt acgtgagtat ttaaaaaagt gggacaaggg caatgatata
     2401 ttagagtttg aaacatccag tgctacagtt gaactggcag cacaagcttt aggagtcgat
     2461 cctgcaagga tagcaaagac tatttcattt aaaaacaagg aaggtgcgat gctgatagtt
     2521 gcagcaggtg atgtaaaaac agataatgga aaattcaaag cagaattcgg ttttaaagca
     2581 aagatgttga cacctgagga gactcttgaa tatacgggat atgctgttgg cggagtttgt
     2641 ccttttggtt taaaaaatga catacctgta tttcttgata tatctcttaa aagatttgaa
     2701 actgtctttc ctgcctgcgg aagcagtaat tccgcaatcg agcttacatg tgaagagctt
     2761 gataaattct ccttgaataa aaagtgggtt gatgtttgca aagggtggac ttgatttcaa
     2821 aaaaaggatg ggaaaaataa tgacataggt aatttaaaat agcgatgctg ctacccaata
     2881 aagaatacat cattaatcaa catgtaaaca tcaggggttt ggatgttttg ctatcaagtt
     2941 ttgcaatagt ggaaaacaaa agcaaattgt ggttgatgta tgaagacaag gtattaatcg
     3001 gggataattt ttatgacgaa ttccgtttga caaggattca agagaagtcc caattcaatg
     3061 ctcttttaaa gttagtttcg gtaaacagga cattgggaca aaaattacat atcgcagcta
     3121 accgcttcag tgactgaaat gtctggttgc ggggtatcaa atgctatgtt taagggagat
     3181 aacagtataa tagcagataa tgagggttag gtttttgaag tcttttatgt ataaattttg
     3241 actcataaaa tgtaccatgc atcatagcct gaatccagga taacgtagtt aaaatccact
     3301 ttcatatcat gatacagata cattgcaaaa tgctgtagaa agagcattaa atcaaataga
     3361 tgacaaaaat tatgcagatg agcttaaaat tgcagggatc aaagaaataa ttaaagtggg
     3421 aattacattt tgtggtaaag aggtaagcct gggatacagg aatgaggtat ttaagttata
     3481 ataaatgcaa tcaaaggagt taaacctttg aaacagatgt ttcaaaatgg gacggctagt
     3541 gttcaaaacc ttgttaagca aatatgatga actaataaag attattggac gatacaaaaa
     3601 gataatagat acacccaata cagatattac ttggaagaaa gtaagcggaa gaatggatag
     3661 atagagaagt taatgctatc acagaggt
//
LOCUS       DAOSDD010000349         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8878_length_3672_cov_3.231684, whole genome shotgun sequence.
ACCESSION   DAOSDD010000349 DAOSDD010000000
VERSION     DAOSDD010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_3672_cov_3.231684"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PK733_18810"
     CDS             complement(<1..788)
                     /locus_tag="PK733_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ12621.1"
                     /translation="MNLDIRRKIGFNFFDVFIYIFCLSFAFISFYPIWYVFLGSISPY
                     ENVTATIMPLIPDFKPTFQYYKHIFARSMFQKALIMSLLKTVLASVGSITVTSMLAYA
                     VSKKHMKGMKFINVMVIFTMFFSGGLIPYYLLMKNLKLLGSFWAMVIPYFFSTYHFII
                     LRNYYSYSIPHALEDAAVIDGANDMILFFRISIPVSMPVIAALLLFETVGHWNDWTGY
                     MIYVDNPRYMPFMYLVQISLKSIGNMNTGQSGIQLADELTFSPPL"
     gene            complement(886..1830)
                     /locus_tag="PK733_18815"
     CDS             complement(886..1830)
                     /locus_tag="PK733_18815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ12622.1"
                     /translation="MRTNFQHRSYHSRILALLKYFRKHWFFYLLTFPGIVMIIVFYYS
                     PMYGLLIVFKNYKFSKGILGSPWVGFDNFRVIFQEKEFYKVLLNTIYINLVNITFGFA
                     FVIFLALMINEVKVGWIRRTIQTAVYLPHFISWVVYAGLVTLFLSPSDGVINKIIQAF
                     GGEPVYFLISNQHFRWIIVISSLLKNAGYSTIIYLAALTGVNPELYECAIIDGAHRGH
                     LLWYISLPRIKPTIAVLAIMNTTSLFSSNFEQVFNLYSPVVYETGDVLSTYIYRTGIM
                     EGMFEQAAALGTIFSIIGLINIIITNKFVKKMDVTGIF"
     gene            complement(1909..3177)
                     /locus_tag="PK733_18820"
     CDS             complement(1909..3177)
                     /locus_tag="PK733_18820"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ12623.1"
                     /translation="MSGQGNKLTVHERGADGIELNAATAEKWRNGEGLLVRDFYPRSI
                     IKLKDGKLLTATGQISIDGGRTWSSKRKLINGEDINVYSLIRMSNGELGCCHADIASM
                     DTACGNANNNWFFRWSNNEGENWSQPVKISLDGLTMGLNNSMFSLKDGRVLVVTYSQF
                     LSSQKGWGASWGTYKGIRMQTETEGHFGQMEVVRVYYSDNFGRSWKACDGWIMGYRGG
                     YEKWVDSFVEATGVELSDGKILLMGRSLVGRIFKSISSDRGETFEYAQPTSLVTSDSP
                     GTLKALPNGDLLFVWNQISREENRRGFRRCRLSSAISKDEGNTWSYFKNIYSIKTLSD
                     RCYIPEEPVMTPVTGDDEVGELPEDFELWHYCDINVIGNEVFLSYINNTCDVEKIDEK
                     DNYCEKKCISYKIKDIVTADTLILPIEWFY"
BASE COUNT         1237 a          714 c          524 g         1197 t
ORIGIN      
        1 agcggaggag aaaaagtcaa ttcatctgcc agttgtattc cactttgccc ggtattcata
       61 ttaccaatgc tttttaatga tatttgtaca agatacataa atggcatata tcttggatta
      121 tcaacataaa tcatatagcc cgtccaatca ttccaatgtc ctactgtttc aaaaagtaat
      181 aatgctgcaa taacaggcat actaacaggt atactaattc tgaagaacaa tatcatgtca
      241 ttggctccat caataacagc agcgtcttcc agagcatgag gtattgaata tgaataataa
      301 tttctgagta taatgaaatg gtatgtgcta aaaaaataag gtattaccat tgcccaaaag
      361 gatcctaaga gcttgagatt tttcattaaa agataataag gtattaatcc gcctgagaaa
      421 aacatggtga atattaccat tacatttata aatttcatcc ctttcatgtg cttttttgaa
      481 actgcataag caagcatgga tgtaaccgtt atggacccta ctgatgccag aacagttttt
      541 aataaagaca taataagagc tttctgaaac atgctcctgg caaatatgtg tttgtaatac
      601 tgaaaagtag gcttgaaatc aggtattaac ggcattatgg tagcggttac attttcatat
      661 ggagatattg agcctagaaa aacataccat atgggataaa atgatataaa cgcaaatgat
      721 aagcaaaata tatatataaa cacatcaaaa aaattaaacc ctatttttct tcttatatca
      781 agattcataa aatcccccat tccaccacta ttaatggcat cacaaatagt gaagaaaatt
      841 cgtttttcaa gcaaatcact ttcatgactt tttgtaaatt aatgattaaa atataccagt
      901 tacatccatc tttttaacaa atttattagt aataataata tttattaatc caattatact
      961 gaaaatcgtt cccagcgcag ctgcttgttc aaacatacct tccattatac ccgttctgta
     1021 aatataggta gaaagaacat ctccggtttc ataaacaaca ggcgagtata aattgaatac
     1081 ctgttcaaaa ttcgatgaaa acaaggaagt ggtattcatt attgctagta ctgctatggt
     1141 tggtttaatt cttggaagcg aaatgtacca aagtagatgt cctctgtgag caccatcaat
     1201 aattgcacat tcatatagtt caggatttac gcctgttaat gctgctaaat atattatagt
     1261 tgaataacct gcgtttttta gtaatgaaga tattacaata atccacctga aatgttgatt
     1321 tgatattaaa aaataaacag gttcacctcc aaaagcttgt attatcttat ttataacacc
     1381 atctgaagga gacaggaaaa gagtaaccag gccagcatat acaacccaag atataaagtg
     1441 tggaagatat acggcagtct gaatagttct tctaatccat ccaactttaa cctcattaat
     1501 catcaaagca agaaatataa caaaagcaaa cccaaatgta atatttacta aatttatata
     1561 tatagtattt aataatactt tatagaattc tttttcctga aaaataaccc taaaattatc
     1621 aaatcctacc cagggacttc caagtattcc tttagaaaat ttatagtttt taaaaacaat
     1681 cagcaaacca tacatgggag aataataaaa tactataatc attacaatac caggaaatgt
     1741 aagcaagtaa aaaaaccaat gttttctgaa atatttaagc agagctaata ttctggaatg
     1801 ataagatcta tgttgaaaat tagttctcat ctgcatacct cctccaattg ttttaattat
     1861 aatactcttt taccagtcat gtgtgtgtta tattatgaaa aatgtgtgtt aataaaacca
     1921 ttctattggc aatatcagtg tatcggctgt gacaatatcc ttaattttat atgaaataca
     1981 ctttttttca caataattat ctttttcatc tattttttca acatcacatg tattatttat
     2041 atatgacaaa aaaacttcat ttccaattac attaatgtca caataatgcc ataattcaaa
     2101 gtcttcaggc aattccccaa cctcatcatc gccggtaacg ggagtcataa caggttcttc
     2161 tggtatatag catctgtcag ataatgtttt tatactgtat atgtttttaa aatagctcca
     2221 tgtatttccc tcatcttttg atattgcact agacaagcgg cagcgccgaa atcctcttct
     2281 attttcctct ctggaaatct gattccaaac aaaaagcaag tcgccatttg gaagagcttt
     2341 aagagttccc ggagaatcgg atgtaacaag gcttgtaggt tgcgcatact caaaagtctc
     2401 ccctctgtca ctgctgatac tcttaaatat tctacctaca agagaacggc ccataagaag
     2461 tattttacca tcgcttaatt ctactccagt ggcctcaaca aaactatcca cccatttttc
     2521 atagcctccg cggtaaccca ttatccaacc atcacatgct ttccatgacc tgccaaaatt
     2581 gtctgaatag tacaccctta caacttccat ctggccaaaa tgtccttctg tttccgtttg
     2641 cattctaata cctttgtaag ttccccatga agctccccat cccttttgtg aagaaagaaa
     2701 ctgactatat gttacgacca gcactcgtcc atcttttagt gagaacatag aattattcaa
     2761 tcccatcgtt aatccatcaa gagatatctt gacaggttgc gaccagttct caccttcatt
     2821 atttgaccac ctaaagaacc agttgttgtt cgcatttcca catgctgtat ccatactggc
     2881 tatgtcggca tgacaacacc ccaattctcc attactcatg cggataagac tataaacatt
     2941 tatatcttct ccattgatca actttctttt actgctccat gttctacctc catcaattga
     3001 tatctgacct gttgccgtta gaagcttacc gtcctttagt ttaataatgg agcgaggata
     3061 aaaatccctg acaagcagac cctctccatt cctccatttt tctgcagttg cagcattaag
     3121 ttcaattcca tcagctcctc tttcatgtac agttagcttg tttccttgtc cactcataat
     3181 atttccccct gataagaatg ttattataaa aattcatcat taataatttt cttgtttcat
     3241 agtaaaagaa gcaatttcgc ttttctcaag gtttcagtgg agattttgca accccacgct
     3301 gaacaaactt gctactataa cttgcgtcaa tccagctctc gcatatcgag gtgaaagact
     3361 tcttcatgct ttggttaaat ttgattttgc ataactaacg ttctctttcc aactttttag
     3421 aaaatattta taaattagtt tgataaccat attagattgc accaggttgc aacgaagtag
     3481 catctccctg caggttatcc tgcaggaaaa gccgtgaaaa ggctttctgg ggaacaatca
     3541 taatattagc atttaatagt attaacatct aataatatac tatcatagct aataaaaaaa
     3601 atgtggctta tttttctata tatgtgtgaa aatattatag atatgttgca tgaaagtact
     3661 attaaattta ag
//
LOCUS       DAOSDD010000350         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8919_length_3657_cov_4.286507, whole genome shotgun sequence.
ACCESSION   DAOSDD010000350 DAOSDD010000000
VERSION     DAOSDD010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8919_length_3657_cov_4.286507"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK733_18825"
     CDS             complement(<1..388)
                     /locus_tag="PK733_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ12624.1"
                     /translation="MFQIKENIYWTGIKDWELRKFHGHELSTHRGSTYNSYVIKDDKT
                     VLVDTVWTPYREEFVENLDKDIGLENIDMIVINHMEPDHGGSLGYLMSKVSENIPIYC
                     TRNGADIIKRHYDKEWNFNIVNTGDTV"
     gene            complement(448..1119)
                     /locus_tag="PK733_18830"
     CDS             complement(448..1119)
                     /locus_tag="PK733_18830"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOJ12625.1"
                     /translation="MIHLEVIDDKIELVEYSDDASLQELSDEVNSFRLKNALKTRDCK
                     GCGECCNQPIPVFGLDLINIQKKTGLELNDVLSRYVVLPERPDLDERCRGIEDFMRTC
                     GLDETEAAIMYEYNQCEPLILPRKKDGTCEFLDKKTGLCTIFNYRPFTGGLYLCNMGD
                     ELSIIQEMVVRHGTWHAYSILGWISEKEIGYNPFLKVDSYDKIYIKDFNFNPEEALGK
                     LFFYF"
     gene            complement(1186..1809)
                     /locus_tag="PK733_18835"
     CDS             complement(1186..1809)
                     /locus_tag="PK733_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12626.1"
                     /translation="MVSTRHGALGLKEDKRVKTVASAVFTLFYTVLSMLFLIRGNYVY
                     ILESAGIYSIYLVYFYTERKGKVFINGYVLILCFIMGMVHSFFGHYLMAYDTSVYFDK
                     ILHFYAIFSFTMASYSLMCNSSSFNNSSKKWILLTMVMYGTAIGAIHEIVEFSLDTFI
                     GTQSQKGLLDTNLDLVADFLGALTAGIIVAYTKIIDYINIKYEDKND"
     gene            2066..2611
                     /locus_tag="PK733_18840"
     CDS             2066..2611
                     /locus_tag="PK733_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOJ12627.1"
                     /translation="MKLKFFKLSLKEMILVSIFAALTAVFSQISIQIPFSPVPITMQV
                     LAVCLAAAILGKKCGTLSQIIYILIGIAGFPVFSGFKSGLGSLLGPTGGYILSFPFSA
                     FIIGYTIEKQTKPGSMQMFFAMLSGLAVCYFFGTLWLGYTLKLSFSKALLMGIEWYLP
                     LDMVKLFMASFLACEVLNSLK"
     gene            complement(2677..>3657)
                     /gene="tuf"
                     /locus_tag="PK733_18845"
     CDS             complement(2677..>3657)
                     /gene="tuf"
                     /locus_tag="PK733_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOJ12628.1"
                     /translation="RHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTRE
                     HILLARQVGVPYIVVFLNKTDMVDDEELLELVEMEVRELLSSYEFPGDDIPIIKGSAL
                     KALESTSTDPNAPEYKPMLDLMAAVDEYVPTPERAVDKPFIMPVEDVFSITGRGTVAT
                     GRVERGTVKVGDEVEIVGLMDAPRKTVVTGVEMFRKLLDQAVAGDNIGALLRGVQRAD
                     VERGQVIAKPGSIKPHTYFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVDL
                     PEGVEMCMPGDHITMTIKLITPIAMEEGLRFAIREGGRTVGAGTVSKILE"
BASE COUNT         1108 a          757 c          554 g         1238 t
ORIGIN      
        1 taacagtatc acctgtatta actatattga agttccattc cttatcgtaa tgccttttta
       61 taatatctgc accatttctt gtacaatata tggggatatt ttcagatact tttgacatta
      121 aatacccaag gcttcctcca tggtcaggct ccatatgatt tataacaatc atatctatat
      181 tttccagtcc aatatcctta tcaaggttct ctacaaactc ttcccggtaa ggggtccata
      241 cagtatcaac aagcactgtt ttatcatctt ttataacata ggaattataa gttgatcctc
      301 tgtgagttga taattcatgc ccgtgaaatt ttctcagttc ccagtctttt atccctgtcc
      361 aatatatatt ttcctttatc tgaaacatta ttttcaacac ctctactatc tttagtattt
      421 ctcattaata cccattctca agtcgcttta aaagtaaaaa aataattttc ccaatgcctc
      481 ttcaggatta aagttaaagt ctttaatata tattttatca tagctgtcaa cttttagaaa
      541 gggattgtag cctatttctt tctcagatat ccagccaaga atcgaataag catgccatgt
      601 tccatgtctt accaccatct cctgaattat ggacaattca tcacccatgt tgcatagata
      661 aagtcccccg gtaaatggcc tataattaaa tattgtgcat aatcctgttt ttttatccag
      721 gaattcgcat gtaccatcct tttttcgtgg caatattaaa ggttcacact gattatactc
      781 atacataatc gctgcttctg tttcatctag cccgcatgta cgcataaaat cctcaatacc
      841 cctgcatctt tcatcaagat caggtcgttc tggaagaacc acatacctgg acaatacatc
      901 atttaattca aggcctgtct ttttttgaat atttataaga tccagcccga aaaccggtat
      961 gggttggttg cagcactccc cacagccttt gcaatccctg gtttttaaag catttttcaa
     1021 tctaaaagag tttacttcat cagataattc ctgtaaagaa gcatcatcag aatattctac
     1081 taattctatt ttgtcatcaa taacctcaag atggatcatc ctgccacact ccttgcatac
     1141 ttgtcagggt gcatttaatt tatatgaaga aatgcacctc tcatattagt catttttatc
     1201 ctcatattta atattaatat aatctataat ctttgtatat gcaactatta ttccagctgt
     1261 taaagcgccc aaaaagtctg ccaccaaatc aagattagta tccagcagac ctttctgact
     1321 ttgtgtccct ataaaagtat ccagagagaa ttctacgatt tcatgtatag ccccgatggc
     1381 agtaccatac attaccatag taagcaaaat ccattttttc gaactattat taaatgaaga
     1441 agaattacac attaatgaat aagaagccat agtaaaggaa aatatggcat aaaaatgcag
     1501 tatcttatca aaatatacgg atgtatcata tgccatcaaa taatgtccaa aaaaactgtg
     1561 aaccattccc attataaaac aaagaataag cacataacca tttataaata cctttccttt
     1621 tctttccgta taaaaataaa ccaggtaaat tgaatatata ccagctgatt caagtatata
     1681 cacataattt cctcttatta aaaacaacat tgaaagaact gtataaaaca aagtaaatac
     1741 tgcagatgca actgttttaa ctctcttgtc ttcctttaaa cctaatgccc catgcctcgt
     1801 agaaaccata gatgtgtacc tctttaacat tttgtatttt attaattgaa cctgtagtga
     1861 gtactaatta tgcaggtata atagtattat acttttttgg caaatatatt caaggcttat
     1921 ttgctcaaag ttattcccca tattgacatt acagtacagt acaggctatt atattcttgt
     1981 acctattgtt aacctgtttt tgattttgtg ttaacaattg tgttataatc acataaaatt
     2041 atattgactt tgggggcagt tttctatgaa gctaaaattt tttaaactct ctttgaaaga
     2101 aatgatactt gtatcgatat ttgcagcatt aacagcagtt ttttctcaaa tttcaattca
     2161 gataccattt tctcctgtac ctataacaat gcaggttctg gcagtatgcc tggcagcagc
     2221 catattaggt aaaaagtgtg gtaccttatc ccaaattatt tatattttaa ttggcatcgc
     2281 cggatttcct gttttttcgg gttttaaatc cggtctaggt tctcttttag ggcccactgg
     2341 aggatacata ttaagctttc ctttttcagc ttttattata ggatatacaa ttgagaagca
     2401 gacaaagcca ggaagtatgc aaatgttttt cgcaatgctg tcagggcttg cagtatgcta
     2461 cttttttggt accctgtggc ttggctatac tttaaaatta agcttttcta aagcgctttt
     2521 aatgggaatt gaatggtatc ttcctcttga tatggtgaaa ctttttatgg cttctttttt
     2581 agcttgtgaa gtcttgaaca gcctcaaata acttttttgc agcaaaaaag ccggttacac
     2641 cggctttttt tataatttac actcaattca aattaattac tcaagaattt ttgaaactgt
     2701 tccagcacca actgttcttc caccctcacg gatagcgaac cttaaacctt cctccatagc
     2761 aatcggagtg ataagtttta ttgtcatagt aatgtggtcg ccaggcatgc acatttcaac
     2821 gccttcaggt aaatcaacaa ctccggtaac atctgttgtt ctaaaataga actggggtct
     2881 gtaaccatta aagaaaggag tgtgtcttcc accttcctct tttgttaata cgtaaacctg
     2941 agcttcaaag taagtatgag gtttaattga accaggttta gcgattactt gtcctctttc
     3001 aacatcggct ctctgaacac ctctaagaag tgcaccaatg ttatcaccgg caactgcctg
     3061 atcaagcagc tttctgaaca tttctacacc tgtaacaact gtttttctcg gagcatccat
     3121 taatcctact atttcaactt cgtcaccaac ttttacagtt cctctctcta ccctacctgt
     3181 agcaacagta ccacgacctg ttatggagaa tacgtcctca acaggcatta taaatggttt
     3241 gtccacagct ctttcaggag ttggaacata ttcgtcgaca gcagccatta aatccagcat
     3301 tggcttgtat tcaggtgcat taggatctgt tgatgtgctt tcaagagctt ttaaagctga
     3361 acctttgatt atggggatat catcacccgg gaactcataa gaactaagaa gttctctaac
     3421 ttccatttca acaagttcaa gaagttcttc atcgtcaacc atatctgttt tattaaggaa
     3481 aacaacaata tatggtactc caacctgacg tgcaagaaga atgtgttctc ttgtctgagg
     3541 cattgggccg tcagcagcag aaacaacaag aatagcaccg tccatctgag cagcacctgt
     3601 gatcatgttt ttaacatagt cagcgtgacc agggcagtca acatgggcat aatgtct
//
LOCUS       DAOSDD010000351         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8958_length_3644_cov_7.735860, whole genome shotgun sequence.
ACCESSION   DAOSDD010000351 DAOSDD010000000
VERSION     DAOSDD010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8958_length_3644_cov_7.735860"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2992)
                     /locus_tag="PK733_18850"
     CDS             complement(<1..2992)
                     /locus_tag="PK733_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12629.1"
                     /translation="MRKGYKFILKQTMAFIVITLLMFQIITGHCSILLVFAEPIGNEN
                     AEMQDSDILKALNADPSFEKPNENNWTFWKAGDTVMEFSTEQTKTGDYSLKISNIAIG
                     GPHVNVTPIKPGKYKFTASYYSPEGTNTAGKIYLNANYKDKDGKTIKNAPEGTRYNIK
                     DTAGGWHTIERDLYVDSERVVYLHMLVIVENIAGDEAIYIDDVILREDEGSEEYIELL
                     KNPDPSFEKPTENNWTFWKASNSVMEFSTEQAKTGEHSLKVSNLTLGGPHRHVPLIEP
                     GTYKLTASYYTPEGTDTAGEIYLNAAYKNVDGGTIETLTDGIKYKIKDTEGSWNTIER
                     VITVESEAVKLVHILLIAGGIEENKVVYIDDVGLYKVEPEEPEEPEEPEEPEEPEEPE
                     EPEEPEEPEEPEEPEEPQALNADPSFEKPNENNWTFWKAGDTVMEFSTEQTKTGDYSL
                     KISNIAIGGPHVNVTPIKPGKYKFTASYYSPEGTNTAGKIYLNANYKDKDGKTIKNAP
                     EGTRYNIKDTAGGWHTIERDLYVDSERVVYLHMLVIVENIAGDEAIYIDDVILREDEG
                     SEEYIELLKNPDPSFEKPTENNWTFWKASNSVMEFSTEQAKTGEHSLKVSNLTLGGPH
                     RHVPLIEPGTYKLTASYYTPEGTDTAGEIYLNAAYKNVDGGTIETLTDGIKYKIKDTE
                     GSWNTIEREITVENEAVKIVHIILVVENIEEDKVIYIDDVSLYREKELEPEDPSIPKM
                     LNADPSFEKPDRNNWTFWKAGDTVMEFSTEQAKTGDRSLKISNLTIGGPHTNVDSIKP
                     GKYRFTASYYSPEGTDTAGKIHLNLIYRDKDGNIMLNAPEGINYNINNTAGDWHTIER
                     DLYVYSEQVKYLHILVVVERIKANEVVYLDDVGLYELEEEPGEPGEPGEPSEPSEPSE
                     PSEPSQPSVTNGSNQSSGQTPVMNQLLNKGSSFEEPERKLWTFWTHEGAVMEYSTDRP
                     KTGNYSFKISGLTIGGPHMNVTPID"
     gene            complement(3440..>3644)
                     /locus_tag="PK733_18855"
     CDS             complement(3440..>3644)
                     /locus_tag="PK733_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12630.1"
                     /translation="GHGNYGIYALLAAAIDERIERVKTENGLTSFSQLVENRYYKPDD
                     IVSIMLPEVLKYFDLPELRTWVK"
BASE COUNT          931 a          773 c          536 g         1404 t
ORIGIN      
        1 gatctattgg cgttacattc atgtgaggtc cgccaattgt taatccagat attttaaaac
       61 tatagttccc ggttttgggt ctgtctgtac tatattccat cacggctcct tcatgagtcc
      121 agaatgtcca tagtttcctt tcaggttctt caaaagatga gcctttattt agcagttggt
      181 tcataactgg tgtttgccca cttgactgat ttgatccatt tgtcacactt ggttggcttg
      241 gctcacttgg ctcacttggc tcacttggct cacttggttc acccggttcg cccggttcgc
      301 ccggttcttc ttccaattca tataaaccaa catcatcaag atatactact tcatttgctt
      361 ttatgcgttc aaccactact aatatatgaa ggtattttac ttgttcacta tatacataca
      421 aatctctttc tattgtatgc caatcaccag ctgtattatt tatattgtaa ttaatacctt
      481 cgggtgcatt tagcataata tttccatctt tatctctata aattaaatta aggtgtattt
      541 tccctgctgt atctgtgcct tctggcgaat agtaggaagc tgtaaatcta tattttcccg
      601 gttttattga gtctacgttt gtgtgaggtc ctccaattgt taaatttgat attttcaggc
      661 tgcgatctcc cgtttttgct tgttctgtac taaattccat cacagtatca ccggctttcc
      721 agaaagtcca gttgtttcta tccggttttt caaaagatgg atctgcatta agcattttag
      781 gaatacttgg atcttctggt tccagttctt tttccctata caagctaaca tcatcaatat
      841 atattacttt atcttcttct atattttcaa ccaccaaaat tatgtgaaca attttcacag
      901 cttcattttc tacagtaatt tctctttcaa tagtattcca gctgccttct gtatctttta
      961 ttttgtactt gattccatca gttagggttt caattgtacc cccatctaca ttcttatatg
     1021 ctgcattcag atatatttca cctgcagtat ctgtcccttc aggtgtatag taagaagccg
     1081 ttaatttata tgtacccggt tctataagcg gaacatgcct gtgcggtcct ccaagtgtca
     1141 aattcgacac ttttaagctg tgctctccag tttttgcttg ttctgtgctg aattccatta
     1201 cactattact ggctttccag aaagtccaat tattttctgt cggcttctca aaagatggat
     1261 ccggattttt cagtagctcg atatattctt cagaaccttc atcctcacgc aaaataacat
     1321 cgtcaatata tattgcttcg tctcctgcaa tattttcaac tataaccaac atatgtagat
     1381 atactactct ctcactatct acatataagt ctctttctat tgtatgccaa ccaccggctg
     1441 tatcctttat attatatcta gttccttcgg gtgcattttt tattgtcttc ccatctttat
     1501 ccttataatt tgcattaagg tatattttcc ctgcggtatt tgttccttca ggtgaatagt
     1561 aagaagctgt aaatttatat tttcctggtt ttattggggt tacattaaca tgaggtcccc
     1621 ctattgctat atttgatatc ttcaggctgt aatctcccgt ttttgtctgt tctgtgctaa
     1681 attccattac agtatcgccg gccttccaaa atgtccagtt gttttcattt ggtttttcaa
     1741 aagatggatc tgcattaagt gcttgcggtt cttccggctc ttcaggttct tccggctctt
     1801 caggctcttc aggctcttcc ggctcttcag gctcttcagg ctcttccggc tcttccggtt
     1861 cttccggttc ttccggttct actttgtaca agccaacatc atcaatatat actaccttgt
     1921 tttcttcaat accccctgct attaatagaa tatgaaccag ttttacagct tcactttcta
     1981 cagttattac tctttcgata gtattccagc tgccttctgt atcttttatt ttgtacttga
     2041 ttccatcagt tagggtttca attgtacccc catctacatt cttatatgct gcattcagat
     2101 atatttcacc tgcagtatct gtcccttcag gtgtatagta agaagccgtt aatttatatg
     2161 tacccggttc tataagcgga acatgcctgt gcggtcctcc aagtgtcaaa ttcgacactt
     2221 ttaagctgtg ctctccagtt tttgcttgtt ctgtgctgaa ttccattaca ctattactgg
     2281 ctttccagaa agtccaatta ttttctgtcg gcttctcaaa agatggatcc ggatttttca
     2341 gtagctcgat atattcttca gaaccttcat cctcacgcaa aataacatcg tcaatatata
     2401 ttgcttcgtc tcctgcaata ttttcaacta taaccaacat atgtagatat actactctct
     2461 cactatctac atataagtct ctttctattg tatgccaacc accggctgta tcctttatat
     2521 tatatctagt tccttcgggt gcatttttta ttgtcttccc atctttatcc ttataatttg
     2581 cattaaggta tattttccct gcggtatttg ttccttcagg tgaatagtaa gaagctgtaa
     2641 atttatattt tcctggtttt attggggtta cattaacatg aggtccccct attgctatat
     2701 ttgatatctt caggctgtaa tctcccgttt ttgtctgttc tgtgctaaat tccattacag
     2761 tatcgccggc cttccaaaat gtccagttgt tttcatttgg tttttcaaaa gatggatctg
     2821 cattaagtgc ttttagaatg tctgaatctt gcatttcagc attttcgtta ccaattggtt
     2881 ctgcaaacac tagcaatata ctacaatgac ctgtgattat ttggaacatc aataatgtaa
     2941 taacaataaa cgccattgtt tgttttagaa taaacttgta tcctttcctc ataaatctac
     3001 ccccctatag ctttgtttaa tagataaaaa tataatgata ttttattatt tttgaagacc
     3061 tcccctcatt acttattaat aaaattgaag actacatatt tatattctaa acagtgacta
     3121 aattaaaaga aatttacaaa acatgagtac tacataaata ggttagcata gaatttgcta
     3181 aaagtcaata tattttagac tacttttcgc aaattatatt ctaattttag cgactatgct
     3241 tttaattttt ttcgaaacat gtgttataat atattctact tgccagtgtt tactgattct
     3301 tgcaatcacg ccataactaa tacttataat tatttgttag attgtaaaaa tatatattaa
     3361 cattcggact tataattgta tgagtacttt cgaaacatac gagtgttcct taagtacaaa
     3421 ttcctaaact ctagtcgttt tattttaccc atgtacgtag ttcaggtaaa tcaaaatact
     3481 tcaatacttc aggaagcatt atactcacta tatcatctgg tttgtaataa cgattttcaa
     3541 ctagctgaga aaaactcgtt aatccattct ctgttttaac ccgttctatc cgttcatcaa
     3601 tagcagcagc taacaaagcg taaattccat agttaccatg ccca
//
LOCUS       DAOSDD010000352         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8968_length_3642_cov_3.207694, whole genome shotgun sequence.
ACCESSION   DAOSDD010000352 DAOSDD010000000
VERSION     DAOSDD010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8968_length_3642_cov_3.207694"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK733_18860"
     CDS             <1..314
                     /locus_tag="PK733_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12631.1"
                     /translation="YMQNLHLCIKYLKILNMIREGSPGSNVNLKVSVEEASAPVSKVY
                     LSVNMYGVFEVLKKEGNNLFSLNFYIPYDAPPGNYNVSLWAVSDSGEKGPVTNVTFMV
                     L"
     gene            823..1863
                     /locus_tag="PK733_18865"
     CDS             823..1863
                     /locus_tag="PK733_18865"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOJ12632.1"
                     /translation="MLKKEVKSNCIKRTGNIIKTVNKMSRTNKVSRNCRINSCLIIVF
                     TMVLLFHTLTGCSSSSSKKSDSTAAGPTSATTGFSGKSSDTKDNESRNYQSQVTKSYD
                     DGAKETKENIKKVIKNGEIAVEVKDVEDAYPKIIEIVEDVGGEEFDKYFSVSEEYKRM
                     ELVLKIPPEKLDLFEQKLKECVGDGKIKRSNIRSQDITSEYYDYTARFESYQGSRDQL
                     RELQRKAETVEDTLRILSELTRLQADIDSMQGQIKMWDKLINMATITLYIDEEANPMK
                     KTTTVGWRFNSPSEIWMTMKNGFISVINWLYSLLIWILITIVSISPILLVAGVVIYII
                     YHMKRKKKGRQM"
     gene            2408..>3642
                     /locus_tag="PK733_18870"
     CDS             2408..>3642
                     /locus_tag="PK733_18870"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ12633.1"
                     /translation="MMNETELSKLAMNGGPKTRIKPFPGRSLLGPEEKAAVNALFDKA
                     ISSGEAFGYNGAEEEAYCKEFAEHMGGGYADAVNSGTSAVYVALRALDIEPFTEVIVS
                     AITDPGGTMPIALINCIPVIADSAPGKYNTDAEQIEKLISPLTSAIVVSHIGGEPCNI
                     DVIAALGKKHNIPVVEDCSQSHGAKLNGKLLGTFGEIAAFSTMFGKHHCTGGQGGIVY
                     TQSEDLYWKIRRASDRGKPFGLPAGSTNAAASLNLNLNDLSAAIGREQLKKLTGIVES
                     RCKFAEKLKEGFKDLKTLKIPDLIPGAEPSYWWWRLEVNTDMISCDKETYCRALIGEG
                     ISLNPSYRGAIPHNMDWFKNRSVFGTSGYPWTAPEYKGDRNRQFPCPNAMLSTECQFN
                     LTVYESWGDEEAKDIIKAF"
BASE COUNT         1276 a          539 c          766 g         1061 t
ORIGIN      
        1 gttatatgca gaatttacat ctttgtataa aatatctaaa aattctaaat atgattcgtg
       61 agggttctcc tgggtctaat gtgaatttga aggtaagcgt agaagaagct tctgcacctg
      121 taagcaaggt ttatctttct gttaatatgt atggagtatt tgaggttttg aaaaaagaag
      181 gtaataacct attttctctc aatttttata ttccttatga tgctccacct ggcaactata
      241 atgtatcact ttgggctgta agtgatagtg gtgaaaaagg gcccgttacc aatgttacat
      301 tcatggtatt atagaattgc attatggctt attttactac cccttaataa gcaataggaa
      361 gcaatagaac cgtacccatg ctttccatgc tttcacattg ggcgcatttt ctttttttgt
      421 catatacaga aaatgcgccc aatgtggtat tattgtttaa catgaaacat ttttacaaca
      481 aaattagtca aataagtata caaatgaatt taacacagct tatgacgcag cttattgtta
      541 caaatcaatg atttaaatcg taagtaaagc aacagctaca cataattgat tataggaagc
      601 tcgggaacgg ttcttgattc tgtgtaatta tataagtatt tattatagta taattatagt
      661 ataatagtaa aaaggatgtg tttccaaaca aactaataga agatgacact ggtgatgctg
      721 cacgatacag tattatatag atgctgatac ataacatagg tgcggaagca taaggaaatg
      781 ttacaggaaa acaataataa cagcctaaaa gggggaataa aaatgctaaa aaaggaagta
      841 aaatcaaact gtataaaaag gacaggcaac ataataaaaa cagttaataa aatgagcaga
      901 acaaataaag tgagcagaaa ttgtaggata aacagttgct taataattgt attcactatg
      961 gtattgttat ttcatactct tacaggctgt agttcatctt catcaaaaaa atcagattct
     1021 acagctgcag gaccaacatc agctactact ggtttctctg gtaagtcaag tgatactaaa
     1081 gataatgaaa gtaggaatta ccaaagtcag gttacgaaat cttatgatga tggagcaaaa
     1141 gaaaccaaag aaaatataaa gaaagttatt aagaatggcg agatagctgt tgaggtcaag
     1201 gatgtagagg atgcatatcc aaagataatc gaaattgtcg aggatgtagg gggagaagaa
     1261 ttcgacaaat actttagtgt atcggaagaa tacaaaagaa tggagcttgt ccttaaaatc
     1321 ccgccggaaa aacttgattt atttgagcag aaacttaaag aatgtgtagg agacgggaaa
     1381 attaaaaggt cgaatattag aagccaggat attacttctg aatattacga ttatacagca
     1441 aggtttgaaa gctatcaggg cagcagagac cagcttagag aattgcagag aaaggctgaa
     1501 accgttgagg atacacttag aatactttca gaattaactc gtcttcaagc agatatagat
     1561 agcatgcagg gacagattaa aatgtgggat aaactaataa atatggctac cataacctta
     1621 tacattgatg aagaagcaaa tcctatgaaa aagactacga cagtaggctg gagattcaat
     1681 tcgccttcag agatatggat gactatgaaa aatggtttta tcagtgttat taattggcta
     1741 tatagcctgc tgatatggat acttattact atagtgagca tatctcctat tcttctggta
     1801 gcaggtgttg ttatatatat tatttatcat atgaagagga aaaagaaggg aagacaaatg
     1861 taagtgcaaa cgtactatat gataagtata agcaaactat atgaaaggat gaaaagctcc
     1921 agctttatcg aatatgttta tttttcagta aaatatgtga agcaagtctc atcacaatga
     1981 tgtagtatat gtttgataag catataagtt aagacattta ttttataggt aatacaccta
     2041 gtgcacacgc attatgattg ttccccagaa agttgtttta cggctttcca ctgcagatta
     2101 cctgcagtga gttataactt tattgcaacc tggggcaatc taatatggtt atcatttatc
     2161 atacttgtag gcttaagttt agtgcaatag gtagctaatc tgatatagtt ggacaacatt
     2221 ctgttaaact tactattttt gctgtttaaa tccaggcatt tgaaaaagga ataaaaaagt
     2281 aattttaaag ggaagtgtaa tgcacatgct tattcacttg catctatatg ttgcgatatg
     2341 atttagaagc ggagtggaaa gtaaatggcc gcaaatttaa caaatacttt atttaggagg
     2401 gatatcaatg atgaatgaga ctgagctttc aaaattggcg atgaatggtg gaccaaagac
     2461 caggataaaa ccttttccag gtaggagcct gctgggacct gaagagaagg cagcagttaa
     2521 tgctcttttt gataaggcca tatccagcgg tgaggctttt ggttataatg gagcggaaga
     2581 agaggcatat tgcaaagaat ttgcagaaca tatgggtgga gggtatgcag atgcagttaa
     2641 ttcaggtact tctgccgtat atgttgcttt aagggcactg gacattgaac cctttactga
     2701 agtaattgta agtgctatta ctgacccggg cggtactatg cctattgctc ttataaactg
     2761 cattccggta attgccgatt ctgcaccggg caaatacaat accgatgcag aacagataga
     2821 gaaacttatt tctcctctta caagcgctat tgttgtatca catattggcg gagaaccatg
     2881 taacatagat gtgatagctg cattagggaa aaaacataat attcctgtgg tagaagactg
     2941 ttctcaatct catggtgcaa aacttaatgg aaaactattg gggacttttg gggaaatagc
     3001 agcattttcg actatgtttg gaaaacatca ctgcactggt ggacagggcg gaatagttta
     3061 tacacaaagt gaagatcttt attggaaaat aaggagagct tcagaccgtg gaaaaccttt
     3121 tggactccct gcaggttcaa cgaatgcagc agcttctctt aatctgaatc tcaatgattt
     3181 atcggcagct attggtagag agcaactaaa aaagcttact ggaatagttg aatctaggtg
     3241 taaatttgct gaaaaattaa aagaaggatt caaagattta aaaactctta aaataccaga
     3301 cttgattccg ggtgcagaac catcatactg gtggtggagg ctcgaagtaa acacagatat
     3361 gatatcctgt gacaaggaaa catattgcag ggcactaata ggtgagggga tatctcttaa
     3421 tccatcctac agaggtgcaa tacctcataa tatggattgg tttaaaaatc gttcggtatt
     3481 tggaacaagt gggtatccat ggacggcacc ggaatataaa ggggatagaa atcgtcaatt
     3541 tccatgtcct aatgcaatgc tttccacaga atgtcagttt aaccttacag tttatgaaag
     3601 ctggggagat gaagaagcaa aagatattat aaaggcattt aa
//
LOCUS       DAOSDD010000353         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_8985_length_3637_cov_5.738973, whole genome shotgun sequence.
ACCESSION   DAOSDD010000353 DAOSDD010000000
VERSION     DAOSDD010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8985_length_3637_cov_5.738973"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(846..2960)
                     /locus_tag="PK733_18875"
     CDS             complement(846..2960)
                     /locus_tag="PK733_18875"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ12634.1"
                     /translation="MNLFQKKYIIGLVISISLVFMSSCSVKIVSDNLSKSKELTLVKD
                     GKAKAVIVLAEKPSPASLEGMKLLVDHIEMISGTILPVINEQDLLKDIRILDNGCIIA
                     GASIMDDSQHSFILVGESEITKMLGISTENLGQGSILIKTTENSLTLIGPDDITPLDP
                     MGTQYAVTSFMEEYLGIRYLWPGDLGKVVPKASTIKVPEIDITYTPKIVQRGIRTNST
                     AERQLKVGLEALGFDIDDYRDKITLRTDDWWQWHRLGGRMFVPSSEGHAFGRLWSIYS
                     NDHPEWFAMDLSGSRDQSKNTQRSRLCKSNLELIKTIAQLKIEELNNNPTCNTVSLSP
                     NDGSVNTFCMCDQCKALDPNNARKVMLWDNSTGNRVEFEYASLTDRIVYFWNAIVEEV
                     TKVHPNALFTVDAYSVYSAPPVLRKLHPNLIVRFVGSIEYSNEEYRQTSLSDWDEWSK
                     FASRLYWRPNLLHDQGYTGSVTNYVHKLAEDFNYLSKHGMQGTDFDSINNHWATVGLN
                     YYILAKLMWNPDINIDEVVDDYFRSGFGNAAKYVKQYFLRIEELAEREAAKCIPGSIL
                     ANPPLVSHFTSEEIDELGAMLNKAMEAAQSDSDSETIIKRIKFVRIGYDFSVLQAEIY
                     KLLAKAKAGENVDLDNAKGLLAQKYEMMIDIFKNHPLAVNVAYVGYGERGERFSSLGW
                     SGIEPPDMPEADEQGRPIDPKP"
     gene            complement(3055..>3637)
                     /locus_tag="PK733_18880"
     CDS             complement(3055..>3637)
                     /locus_tag="PK733_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12635.1"
                     /translation="NWNANPSALTTGSFVITNKNKYPVETVKWIDYFYTEEGSALYLL
                     GKEGVTYIKNPDGTYDFTDLILKNPDGLDPGQATNKYTPGSVGDNPVVLLKNVFKGAE
                     AQPIPLNAAEKMQKYSLKEVWTQFDLTIEENERVRELSSDINSYVAKTTADFLTGRTP
                     FTEWDNYIAALKKMGLDEFLQIQQIAYERYSNH"
BASE COUNT         1076 a          751 c          524 g         1286 t
ORIGIN      
        1 acatcataaa attcatcagt tgtagtaggc ataccttttc ctacttcctc taatcatttc
       61 ctactaataa atataaatga acctgttcgt acttcccaaa aatctttatc tgcaatatat
      121 acaagtgagt atatataacc atcaggcata gaaattgatt ttctaagtcc tacataattt
      181 acttcatcat ttattattta ctgtgtgttt accaattaag ctgtttagag gtattagcga
      241 accttcagct ccatgccttt gtaactctga tgtgctgata gcacacttca taaatacatc
      301 tggtaaatct ccagtatcca actctaaatt acgtttctct gtaaacggta aaagctgcat
      361 cttccgttaa attccaatgt gacccatatt ttataatttt ttagactctt tccatgtaat
      421 aatgtcatta aatctgcatg tatcggagaa atcttgacat aacttttatg ttattggttc
      481 ttcaacaatt ggaaacattt gagcagcttt tgcttctaaa tttaaatctt ctacttttca
      541 aactgatgca ttctctgtct cattatttga ttgttctgca tctttcacta cgcatcctga
      601 gaaaagtgta actgctataa aaacagttaa tattatagct aacagttttt ttattaaatt
      661 taggtatagc ctcttcgcaa tgaggctata cctctggttc tatcccattt caaaccttaa
      721 acatattatt ttattatatt tatattttgt ctaccccttt ttagcaataa gaacctattt
      781 tgcaataagg accttcatct cagataccat attcatagtc tttaagctaa gaataatcac
      841 aataattaag gttttggatc tattggccga ccctgttcat cagcttcggg catatctggt
      901 ggttcaatac ctgaccagcc caaagaacta aatctttctc ctctctctcc atagcctaca
      961 tatgcaacat tcacagcaag tgggtgattt ttaaaaatgt ctatcatcat ttcatatttt
     1021 tgtgcaagaa ggcccttagc attatctaga tcaacattct ctcctgcctt agccttagcc
     1081 aacaacttat atatttcagc ttgcaataca ctaaagtcat atcctattct aacaaatttt
     1141 atacgtttga ttatagtttc agaatctgaa tcactttgtg ccgcttccat agctttattt
     1201 agcatagctc caagttcatc tatttcttca gaagtaaagt gcgatacaag aggtgggttt
     1261 gctaagatgg aaccaggtat acatttagct gcttctcttt cagcaagttc ctcaatccgc
     1321 aagaaatatt gctttacata tttcgctgca ttgccaaagc ctgagcgaaa ataatcgtca
     1381 acaacttcat ctatattaat atctggattc cacatgagtt ttgctagtat gtagtagttc
     1441 aatcctactg tagcccagtg gttattaata ctatcaaaat ctgttccttg catgccatgt
     1501 tttgacagat aattaaaatc ctctgccaac ttgtgaacat aatttgttac tgatcccgtg
     1561 tagccctgat catgcaagag attcgggcgc caataaagtc tgctggcaaa ttttgaccat
     1621 tcatcccaat ccgaaagtga cgtctgccgg tactcctcgt tggagtattc aattgaacca
     1681 acaaaacgta caatcaaatt aggatgaagt ttgcgaagaa caggaggagc agaatacaca
     1741 ctataagcat caacagtaaa taatgcattt gggtgcacct tggttacttc ctcgacaata
     1801 gcattccaga agtacactat tcgatctgtt agtgatgcat attcaaattc gacacgatta
     1861 cctgtactat tatcccaaag cattaccttt cttgcattat tcggatccag cgctttacac
     1921 tggtcgcaca tgcaaaatgt gtttacactt ccatcattag gtgataaaga aactgtatta
     1981 caagtaggat tattattaag ctcttcaatt tttaactgcg caattgtttt tattagctcc
     2041 aaattacttt tgcataacct ggatctctga gtatttttgc tttgatctct ggaaccacta
     2101 agatccattg caaaccattc tggatgatca ttactatata tactccataa cctaccaaat
     2161 gcatgtcctt ccgaagatgg tacaaacatt cttcctccta atctatgcca ttgccaccaa
     2221 tcatcagtcc ttaatgttat cttgtccctg tagtcatcga tgtcaaagcc aagtgcttcc
     2281 aaccctactt ttagttgtct ttcagccgta ctatttgtac ggatgccacg ctgtacaatt
     2341 ttaggcgtat atgttatatc tatttcagga acctttatag ttgaagcttt aggtaccact
     2401 ttgccaagat cacccggcca cagataacgt atacccaaat attcttccat aaaagacgtt
     2461 actgcatatt gtgttcccat tggatctaat ggtgttatat catctggacc tataagtgtt
     2521 aaagaatttt ctgttgtctt tatcagaatg ctgccttggc ctaggttttc agtactgata
     2581 ccaagcattt ttgtaatttc gctttcccca acaagtataa atgaatgttg ggaatcatcc
     2641 ataatcgatg ctcctgcaat aatgcatcca ttatctaata ttctaatatc tttaagcagg
     2701 tcctgttcat ttataactgg taatatagta cccgaaatca tctcgatatg gtcaaccagc
     2761 agcttcatac cttccagcga agccggtgaa ggtttctccg ctaatactat gacagctttg
     2821 gctttgccat cctttacaag tgttaactcc tttgattttg ataagttatc actaactatc
     2881 ttcacagaac aagatgacat aaaaactaag cttatggaaa tcaccaatcc tataatatat
     2941 tttttttgaa ataaattcat aacatatatc tccatctcgt aaatagttga tatttttaat
     3001 tacattcatc ttactttcaa attttagcca aagcatcatt cattttattt ttggttagtg
     3061 attagaataa cgctcatatg caatctgttg aatttgtaaa aattcatcca ggcccatttt
     3121 ctttaatgca gcaatataat tatcccattc tgtaaatggt gttcttccag ttagaaaatc
     3181 agctgttgtt tttgcaacat atgaattaat atcagaagat aattctcgca ctctttcatt
     3241 ttcttctata gtcaagtcaa attgtgtcca aacttctttt aaactatatt tttgcatttt
     3301 ttccgctgcg ttcaatggaa ttggctgggc ttctgctccc ttaaatacat ttttcaataa
     3361 tacaaccgga ttatccccaa cagaaccagg tgtgtactta tttgttgctt gaccaggatc
     3421 caacccatca ggatttttta atatcaaatc tgtaaagtca tatgttccat caggattttt
     3481 tatatatgta acaccttctt ttcctagtaa ataaagtgca cttccctctt cagtataaaa
     3541 ataatcaatc catttaacag tttcaacagg atacttgttt ttatttgtaa ttacaaatga
     3601 accagttgtt aaagctgagg ggttggcatt ccagttt
//
LOCUS       DAOSDD010000354         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9006_length_3631_cov_3.196029, whole genome shotgun sequence.
ACCESSION   DAOSDD010000354 DAOSDD010000000
VERSION     DAOSDD010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9006_length_3631_cov_3.196029"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK733_18885"
     CDS             <1..680
                     /locus_tag="PK733_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ12636.1"
                     /translation="MTLCALLGCAIEKIAYKPLRNATRISALITAIGVSLFLEYSTMF
                     FVKATVRTYPQVLTDITFKPLKDVIVNIQQVFIFSVTIILMILLQYIVHKTKIGKAMR
                     AVSTDKDAAQLMGINVDKTISFTFAIGSALAGAAGILVGIYYNSINPLMGVLPGLKAF
                     IAAVFGGIGVIPGALIGGITIGIIETMVSGYGNSLYKDAAAFAILVIILLIKPSGLLG
                     KNTSEKV"
     gene            693..1664
                     /locus_tag="PK733_18890"
     CDS             693..1664
                     /locus_tag="PK733_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ12637.1"
                     /translation="MKLISDSTKTKYAKHAKYTVSFVLLYTIVQLLIYFNILNDYYQV
                     TLSMICINIILATSLNLITGFTGQFSLGHAGFMSIGAYTCAVITLNISSQYSFIIGAF
                     AGAILAAIIGFLIGIPTLRLKGDYLAIATLGLAEIIRILLLNFDFTGGARGLDSIPQY
                     TNWTWLFVFTVGTILIIQSFIKSSHGRACISIREDEIAAEAVGINSTKYKVLAFVIGA
                     FFAGIAGALYSSYFYYIKPDLFGFLKSIDILVIVVLGGMGSITGSIFAAILLAVISTY
                     LQAFAEFRMVIYSIVLLLIMIFRPQGLMGSKEFKLTELIKRRPTKVN"
     gene            1682..2473
                     /locus_tag="PK733_18895"
     CDS             1682..2473
                     /locus_tag="PK733_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12638.1"
                     /translation="MAILTVKNLTMTFGGLAALTNVNISLEIGGLVGLIGPNGAGKTT
                     VFNLLTGVYTPTSGVVEYHRTEEAPVQIINGLSPHVISGLGISRTFQNIRLFNDLSVL
                     DNIRIAIHQNVKYGLLSTFFRLPSFYREEQEILNRANEILEIFKLETKKDELAKNLPY
                     GEQRRLEIARALATKPSLLLLDEPAAGMNPNETSALRELIKWIWKEYNLTILLIEHDM
                     SLVMKICERIYVLDYGMVIAEGTPEEIRSNKRVVEAYLGGELLDA"
     gene            2466..3170
                     /locus_tag="PK733_18900"
     CDS             2466..3170
                     /locus_tag="PK733_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12639.1"
                     /translation="MLEVNGIDVYYGVIHALKNVSLKVQEGEIVTLIGANGAGKTTAL
                     RTVSGLIKPSKGSIYFRGGKISGINPQEIVAKGISHVPEGRHVFYDMSVSENLELGAF
                     LRRDKDGIKDDYEMIYSKFPILKDRSKQRAGTLSGGEQQMLAIGRALMSRPKLLLMDE
                     PSMGLAPLLVHEIFSIIKEINNQGTTILLVEQNANIALSIAHRAYVIEIGSVVIEGTG
                     KELVKSEEIKKAYLGG"
     gene            3175..>3631
                     /locus_tag="PK733_18905"
     CDS             3175..>3631
                     /locus_tag="PK733_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ12640.1"
                     /translation="MNVKSRMTSNPYIISPDATIAEALEIMRKNNIRRLPVVKNGKLV
                     GIVTEREMQEVTPSKATTLSVFELNYLLSKTKVSAVMTRDVITVSPDSLLEEAAVLMR
                     DNRVGALPVIQDDRVVGIITETNIFDAFIDLLGFRDSGTRISVEVEDSPG"
BASE COUNT         1168 a          574 c          760 g         1129 t
ORIGIN      
        1 ccatgacttt atgtgcttta ctggggtgtg ccatagaaaa aattgcatat aaacctctcc
       61 gtaacgcaac aagaatatca gcgcttataa ctgctatagg agtatctctt ttccttgaat
      121 actccactat gttttttgta aaagctaccg taagaacgta ccctcaggtt ttaacagata
      181 taacttttaa acctttaaaa gatgtaatag taaatatcca gcaggtattt attttttctg
      241 taactattat actcatgatt ctactgcaat atattgttca taagaccaaa ataggtaaag
      301 ccatgagagc agtttctacc gataaagatg cagctcagct tatgggaatt aatgtagaca
      361 agactatttc ctttaccttt gccataggtt ctgccttggc aggtgcagca ggcatattgg
      421 ttggaattta ctataactcc atcaatcctt tgatgggagt tcttcctgga ttaaaggctt
      481 tcatagcagc agtatttggg ggaatcggag ttataccggg agctttgata ggaggaatta
      541 caataggaat aattgagaca atggtaagcg gctacggcaa ctccctttat aaagatgcag
      601 ctgcctttgc aatactggtt attattcttt taattaaacc ttctggatta cttggcaaga
      661 ataccagcga aaaagtgtag gtggtgaata caatgaaact tatatcagat tcaacaaaaa
      721 caaaatatgc aaaacatgca aaatatacag taagctttgt cttattatat accatagtac
      781 agttacttat ttactttaat attctaaatg attactatca ggttaccctt tctatgatat
      841 gcataaatat aatactagca acaagcctta atttaataac tggttttacc ggtcagtttt
      901 ctttgggcca tgcagggttt atgtcaatag gagcctatac ttgtgctgtt ataacattaa
      961 atataagcag ccaatattca tttataattg gagcttttgc aggagcaatt ctagctgcta
     1021 ttataggttt tctaataggg atacctactt taagattaaa aggcgattat cttgctattg
     1081 caacactagg tcttgcggaa attatcagaa ttttattgtt aaattttgat tttacaggag
     1141 gcgccagagg tttggatagt ataccgcaat ataccaactg gacctggctg tttgtattta
     1201 ccgtaggtac aatattaatt atccagagtt ttataaaatc ctcccatggc agagcctgca
     1261 tatctataag ggaggacgaa atcgcagctg aagcagtagg aatcaattca accaaatata
     1321 aggtgttagc ttttgtaata ggggctttct ttgcgggaat tgcaggagct ttgtattcat
     1381 catactttta ttatattaaa ccggaccttt ttgggtttct aaagtcaatc gacattttgg
     1441 taattgttgt gcttgggggt atggggagca ttaccggatc catatttgct gctatacttc
     1501 ttgcagtaat atctacttat ttacaggcat ttgcagaatt tagaatggtg atttattcta
     1561 tagtgctttt attgattatg atttttaggc cacaaggact tatggggtca aaagagttta
     1621 aacttactga acttataaaa agacggccta caaaggttaa ttgatttgag gtgattacta
     1681 aatggctata ctaacagtaa aaaatcttac gatgacattt ggcggactgg cagctttgac
     1741 aaatgtgaat atttccctgg aaattggtgg tttggtagga cttataggac ctaacggagc
     1801 aggtaagact actgtcttta acttattgac aggggtttat acaccaactt caggcgtagt
     1861 tgaatatcat cgtactgaag aagcgccggt acagattatt aatggtttga gtccgcatgt
     1921 aatatccggt cttggtattt ccaggacttt ccaaaatatt agacttttta atgatctttc
     1981 agttttagat aatataagaa ttgctattca tcaaaatgta aaatatggac ttctttcaac
     2041 atttttcaga cttccgtcat tttataggga agaacaggaa atactgaata gggcaaatga
     2101 gatattagaa atatttaaac ttgaaactaa aaaggatgaa ttggcaaaaa atctacctta
     2161 cggagagcaa agaagactgg aaattgcgag agcccttgca acaaaaccct cgcttctatt
     2221 gcttgatgaa ccggctgcag gtatgaatcc aaatgaaaca tcagctctga gagaacttat
     2281 taaatggata tggaaagaat ataatcttac gatacttctt attgaacatg atatgtctct
     2341 tgttatgaaa atttgcgaga ggatatatgt attagattat ggtatggtaa ttgctgaagg
     2401 gacaccggag gaaattcgaa gcaacaagcg tgttgtagaa gcttacctgg gaggtgagtt
     2461 attggatgct tgaagtaaat ggtattgatg tttattatgg agtaatacat gccctgaaaa
     2521 acgtatcttt aaaggtacag gaaggtgaaa ttgtaactct cattggtgct aatggtgcag
     2581 ggaaaactac agctttgagg acagtttccg gccttataaa accttcaaaa ggttctatat
     2641 atttcagagg tggtaaaatc agtggtataa accctcagga aattgtagca aagggtattt
     2701 cacacgttcc agaaggaaga catgtttttt atgacatgtc ggtatcagaa aatctggagc
     2761 ttggtgcttt tttaagaaga gataaggatg gaataaaaga tgattacgaa atgatctatt
     2821 caaaatttcc aatcttaaaa gatagaagca aacagcgtgc aggaacatta agcggaggag
     2881 aacagcaaat gcttgccata ggccgggcgc ttatgtcaag gcccaaactc ctccttatgg
     2941 atgaaccatc aatgggactt gctccccttc tagtacatga aatattttcc attataaaag
     3001 aaataaacaa ccaaggtaca acgatccttc tggttgaaca aaatgctaat atagcgcttt
     3061 ctattgctca cagagcttat gttatagaaa taggttctgt tgttattgaa ggtacaggta
     3121 aagaacttgt aaaaagtgaa gaaattaaaa aagcatattt gggaggttga tcttatgaat
     3181 gttaaaagca ggatgacaag taatccgtat ataatatcac ctgatgctac aatagctgaa
     3241 gcattggaga ttatgcgcaa gaacaacata agaaggttgc ctgtggtaaa gaacgggaaa
     3301 ctggtcggta tagtgactga aagggaaatg caggaagtta ctccttcaaa agctaccact
     3361 ctgagtgttt ttgagttaaa ctacctttta tcaaagacca aggtaagtgc agtaatgaca
     3421 agggatgtaa ttactgtttc tcccgattcg cttcttgagg aagctgcagt tcttatgagg
     3481 gataataggg taggtgccct tcctgtgatt caagatgata gagttgtagg tattataaca
     3541 gaaacgaaca tttttgacgc tttcattgat cttctaggtt tcagggatag cggcacgagg
     3601 atttcagtgg aggttgagga ttccccggga a
//
LOCUS       DAOSDD010000355         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9203_length_3568_cov_4.249360, whole genome shotgun sequence.
ACCESSION   DAOSDD010000355 DAOSDD010000000
VERSION     DAOSDD010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9203_length_3568_cov_4.249360"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1186
                     /gene="hydE"
                     /locus_tag="PK733_18910"
     CDS             125..1186
                     /gene="hydE"
                     /locus_tag="PK733_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOJ12641.1"
                     /translation="MDRHIKVINALEIEHNLTTKDLAYLISDNRTEIIPSILGAADRV
                     RSKYVGNEVHLRGLIEFSNYCHNSCQYCGLRGSNKSIERYRMSASDIVETAEKAANLG
                     LKTIVLQSGEDKFYSVEVLCSVIEEIKKLDVAVTLSIGELATEDYANLKTAGADRFLL
                     RIETTEPELYKRLHPGMSIENRKRCLYDLKALGYEVGTGCLAGLPGQTPLMLAKDLKF
                     FKELDADMIGIGPFIPCPGTPLENETGGSVQLALKMIALTRLMLPGINIPATTALAAK
                     DEEGYKKGLECGANVIMPNMGKNEYRELYKIYPGKGSAEVNVKTCLDAIKELIVKEGR
                     IVGKGEGNRQGFNLTKRQS"
     gene            complement(1234..1389)
                     /locus_tag="PK733_18915"
     CDS             complement(1234..1389)
                     /locus_tag="PK733_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12642.1"
                     /translation="MTKKENAPLLSGKMRITEIASVVGHSNSQYFIRKFKLKFGITPK
                     QFQRSLS"
     gene            1560..2393
                     /gene="zupT"
                     /locus_tag="PK733_18920"
     CDS             1560..2393
                     /gene="zupT"
                     /locus_tag="PK733_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOJ12643.1"
                     /translation="MGSIGSNLDANSVLLAFALTLFAGLSTSIGGLFTVFCRKTNTKF
                     LSVILGFSAGVMIYLSFMEILPEAKSFLIDMLGEPAGLWITIAAFFGGVLIIAIIDKL
                     IPSSDNPHESHKIEEMQGCNANYKNSKLLRTGLFTAFAIAIHNFPEGIATFISALKDP
                     SLGIMIAIAIAIHNIPEGMAVSIPIFCAEGSRKKAFLLSLASGLAEPLGALVGYLLLM
                     PFMNDLILGIIFAAVAGIMVFISFDELLPTAEEYGEHHLAILGLMGGMALMAISQILL
                     I"
     gene            2785..2925
                     /locus_tag="PK733_18925"
     CDS             2785..2925
                     /locus_tag="PK733_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12644.1"
                     /translation="MTEDRGACGYPMYGGCNWIIWLLIILVVICVFCPGIFGGLGYGP
                     RE"
     gene            3140..>3568
                     /locus_tag="PK733_18930"
     CDS             3140..>3568
                     /locus_tag="PK733_18930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HOJ12645.1"
                     /translation="MKKRRININGMTCTACAKAVERSVGKLEGVHSANVNFATEKLDV
                     EYDENIVDIGRIKESINKAGYEAWDEEKQEKKERQYQEKQYIKNKKNGNIKNIIETGK
                     QVYVGRERKENKIRLMWTRFVVALVFTIPLLYIAMGSMIGF"
BASE COUNT         1225 a          488 c          767 g         1088 t
ORIGIN      
        1 aagatgacag atgaaaagag gaagagtttt ataatcgaat ctttaaataa gatggagaag
       61 ggagaaaggg atatcagggt atgacaaaca actgtaccaa aagagaaatg tccccattta
      121 cataatggat aggcatatta aagttataaa tgcacttgaa atagaacata atttaactac
      181 aaaagattta gcttacctaa tatcggataa taggactgaa ataatcccgt caatattagg
      241 agctgctgac cgagtcagaa gtaagtatgt gggtaatgaa gttcatttaa gaggactaat
      301 tgagtttagt aactactgtc ataatagctg tcaatactgt ggtttaagag gttctaataa
      361 gagtattgag agatatagaa tgagcgcttc tgatatcgtt gaaacagctg aaaaggctgc
      421 aaacttaggt cttaaaacta tagttctcca aagcggtgaa gataagtttt attctgtgga
      481 agtcctatgt tctgttatag aagaaattaa aaagcttgat gtagctgtga ctttgagtat
      541 aggggagcta gctacagaag attatgccaa tcttaaaaca gcaggtgcag acaggttcct
      601 tttaaggata gaaacaacgg agccggagct ttataaaaga ttacatcctg gaatgagcat
      661 cgaaaacagg aaaagatgtc tatatgattt aaaagcttta ggttatgaag taggtacagg
      721 ctgtcttgca ggattgccgg gacaaactcc tttaatgttg gctaaagacc taaagttttt
      781 caaggagttg gatgcagata tgataggaat cggaccgttt attccatgtc ccggtactcc
      841 actggaaaat gaaacaggag gatctgttca attagcattg aaaatgatag cattaaccag
      901 gctgatgctt cctggtatta atattcctgc tactactgca ttggccgcaa aggatgagga
      961 aggatataag aaaggtttgg aatgcggtgc taatgtaata atgcccaata tgggtaaaaa
     1021 cgaatatagg gaattatata aaatttaccc cggaaaaggg agtgcagagg tgaatgtaaa
     1081 aacctgcctt gatgcaataa aagaacttat tgtaaaagaa ggtaggatag tgggtaaagg
     1141 cgaaggaaat cggcaaggct ttaacttaac caagcgtcaa agttaggtag aatggcagga
     1201 aaaggcaggg aaaatcgtca tagaaaaatt cattcaagat aaactacgtt gaaattgctt
     1261 cggagttata ccaaacttta atttaaactt tcttataaaa tactgggaat tggagtgtcc
     1321 tacaacactc gcaatttctg ttattctcat tttgcctgat aaaaggggcg cattttcttt
     1381 tttggtcata ttgaaaatct cctctctcat aagtgacatt ttatcagaat aatttcaaca
     1441 tgacattatc atagaataat cacgagatca cgaatccctc ttgacagaag attttatttt
     1501 atttaaaatg ttattgataa tcattatcac tatcactaac agacgcgggg gcatggaaaa
     1561 tgggaagcat aggttcaaat ttagatgcca atagtgtttt acttgcattt gctctgactt
     1621 tatttgcagg tttatctaca agtataggcg gactttttac agtattctgc agaaaaacca
     1681 atacgaagtt tttatcagtt attttgggat tttcagccgg agtaatgata tatctttcat
     1741 tcatggaaat tcttcctgaa gcaaaaagct ttttaattga tatgttagga gaaccggcag
     1801 gtttatggat tactatagct gctttttttg gaggggtgct gattattgca attattgata
     1861 aacttatacc ttcaagtgat aaccctcatg aatctcataa aatagaagag atgcaaggtt
     1921 gcaatgccaa ttacaaaaat agtaaacttt taaggacagg gttgtttact gcttttgcta
     1981 tagcgataca taattttcct gaaggtattg ctacttttat ttcagctctt aaggatcctt
     2041 ccctgggaat aatgatagca attgcaatag ccatacataa tattcctgaa ggtatggcag
     2101 tgtctatacc tattttttgt gctgaaggaa gcaggaagaa ggcttttttg ctttctttgg
     2161 cttcaggctt ggcagagcct ttaggtgcat tggtagggta ccttctatta atgccattca
     2221 tgaatgattt gatattaggt attatttttg ctgcagtagc aggtataatg gtattcatat
     2281 catttgacga gttgcttcct acagctgaag aatatggtga acatcatttg gctattctag
     2341 gccttatggg aggaatggcc ttgatggcaa taagccagat attacttata tagtcacttt
     2401 atatagaact tgatataaca gggttataga ttacagcaga ataacctgca cttagttttt
     2461 ccataccagt gtgttcagaa ccatggcata gatatttaag ttaaaaaatg aagctttgag
     2521 gcaaatttat taccgagtat agtactttac tcggtttttt attgtattgc cgcgaacact
     2581 catgactttg gcctatagtg agcttatagt gtattcaata gttaatatat ttggtaacac
     2641 atatatactg acttgaatat tatacttata gattcagtta aaactactta tctaaataag
     2701 ttaactggag taataaggaa atgtagctta acttatttta acaaatttta ctatttgaaa
     2761 taaaactgaa aggaggtcaa tagtatgact gaagacaggg gtgcatgtgg atatcctatg
     2821 tatggtggct gcaactggat aatttggctc ttaataatcc tggtagtaat atgtgtattc
     2881 tgcccgggca tttttggcgg attaggatat gggcctagag aatgagtcaa tatgaattaa
     2941 acttaacacc tgattaatga catgttggaa aaattatatg ttctgcgtat atcatgcatg
     3001 taataaatat agaaaataat gaaagcgctg gacatataat ttttttatgt agatgagggg
     3061 gtgcatttta cgggtatgca ttgatttgat ataatagcag aagataaaac cgaaaaccag
     3121 caggggagtg aaagttctga tgaaaaaacg aagaataaat attaacggga tgacatgtac
     3181 tgcttgtgca aaggctgtag agagaagtgt agggaaattg gaaggcgtcc attcagctaa
     3241 tgttaacttt gcaacagaaa agcttgatgt agagtatgat gaaaatatag ttgatatagg
     3301 taggattaaa gaatcaataa acaaagcggg gtatgaagct tgggatgaag aaaagcaaga
     3361 aaagaaagaa aggcaatatc aagaaaagca atatattaag aacaagaaaa atggaaatat
     3421 taaaaatatt attgaaaccg gaaaacaggt atatgtcggc agagaacgga aagaaaataa
     3481 aataaggctg atgtggacaa ggtttgtagt agctttagtg tttacaatac ctcttttata
     3541 tattgctatg ggctcaatga ttggcttt
//
LOCUS       DAOSDD010000356         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9225_length_3562_cov_5.640719, whole genome shotgun sequence.
ACCESSION   DAOSDD010000356 DAOSDD010000000
VERSION     DAOSDD010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9225_length_3562_cov_5.640719"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..617
                     /locus_tag="PK733_18935"
     CDS             90..617
                     /locus_tag="PK733_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12646.1"
                     /translation="MQLTYVLELLKPTKRKQKIFLNNIAEVVKNRQTIADKLKAGETK
                     LSCADFKEINLPSAVKNQNIREVKALYKLFLKSDSDKDNLEFKDNQPICYNSQNYKID
                     NHIISIPLYTSQCQRFAFPVKQTKRFKELLQYIENGCKLGKANLYSCECGNHIQADLN
                     ASRNIANKYLEQQSA"
     gene            850..1287
                     /locus_tag="PK733_18940"
     CDS             850..1287
                     /locus_tag="PK733_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOJ12647.1"
                     /translation="MKIFVDGDSCPVVDIAIKIAKEYNLEIIIVKNYNHEIWSDYATI
                     LTVDQGPDSADYYIGNNAVKGDIVVTQDYGLAAIALSKEALCLNQNGLIISSGNIDLL
                     LDRRYFNQQLRRKHKKYTKFKKRTIEQDEKFEQSLKQLIQKSM"
     gene            1343..1705
                     /locus_tag="PK733_18945"
     CDS             1343..1705
                     /locus_tag="PK733_18945"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HOJ12648.1"
                     /translation="MVYQQDWMMRQIESMVQGIARLIFKKDTITYELIDETNYTKTDL
                     YYKELLELLNSSKINEAENLLFERIESSDINYLTIAVDFYNRLNKLSDEQLEKSNFSR
                     VEIKSGLEDVLKIFDLMP"
     gene            1814..3097
                     /locus_tag="PK733_18950"
     CDS             1814..3097
                     /locus_tag="PK733_18950"
                     /inference="COORDINATES: protein motif:HMM:NF026863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4652 domain-containing protein"
                     /protein_id="HOJ12649.1"
                     /translation="MIARVKKKSCIWTIVAVILVLLLAWVGLTKSRYRTQTHDTNNGA
                     NFDGKSQSNILSENPINLTDNLQNHKLVVKMIDGEYYDDEEPGPFMGPNWVGKFAVEL
                     INVKGNVISEFDLNKSFNEAKLRFGSTFEIEFDDYNDDGNLDFTIGQYASSNGRDYKI
                     FTIREDKKIAELPIKDYPTLFISNTTGYYSTRLTKVDKTTFKKEYYDNSESKCFEDVF
                     QWTGKEFVWINKREANGAYVDHVATFDNGGKILYKKNYGNTEIIIENEKKETVLNDNS
                     PSYPIVQQDRKNIAYISPLEWEQIGQIYVYDPEKNTNNAVLGKDDIPKQYTPKKVYWL
                     NEKYLLAVIGFAYGTVDVGGDVYVIDIDTGNYDIYLKADENQQINDIYTVADKIVFLT
                     VEFNEQEKKYIYGDEIIIKHDELLKFAFSLKKGKE"
     gene            complement(3133..3390)
                     /locus_tag="PK733_18955"
     CDS             complement(3133..3390)
                     /locus_tag="PK733_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HOJ12650.1"
                     /translation="MNHKIAVEESLTQVKDFLAQKGYSVDSINFSREYARDLDEYDAV
                     VVTGINENFLGIEDTSTNATVINAKGQTAEQIYSQIEKKLH"
BASE COUNT         1349 a          484 c          624 g         1105 t
ORIGIN      
        1 taaaattatt tatacaatac atatcttaaa cacaaatcct ttttatgata taatacagac
       61 aacaacttat attaaggggt tttgataata tgcaattaac ttatgttcta gagttgctaa
      121 aaccaactaa aagaaaacaa aaaatatttt taaataatat tgcagaagta gtaaagaatc
      181 gtcaaactat cgctgacaaa cttaaagcag gagaaactaa gttgagttgt gctgacttta
      241 aagagataaa cctaccttct gctgttaaga atcagaatat tagagaagtt aaagcactct
      301 ataagttgtt tttaaaatct gattctgata aagataattt agaatttaaa gacaatcagc
      361 ctatttgcta taatagtcaa aattataaaa ttgataatca tataatctca atacctcttt
      421 atactagcca atgtcaaaga tttgctttcc ctgttaagca aactaaaaga tttaaagaat
      481 tgctacaata tatcgagaat ggttgcaaat taggtaaagc taatctatat tcttgcgagt
      541 gtggtaatca tatccaggct gatttaaatg caagtagaaa tatagctaat aaatatttag
      601 aacagcaatc tgcttagatg gtaatagtct aagtgcctaa gttggtatat ccatatctta
      661 ggaaggctga tggcacagct taaagtttgc gtcatacttg cctaccagaa atggactggc
      721 atttaatcag cagacaacct cgtaagttcc tttaaggaat ccttgttcgt gacgatagga
      781 acgggtgggg aggatgtcaa tacttatatt aaccgtattc ccgaaaatag caaagaaatg
      841 gaggtataaa tgaaaatatt tgtagatggg gatagctgcc ctgttgtaga tatagctata
      901 aaaatagcga aagaatataa tttagaaata atcatagtaa agaattataa tcacgaaatt
      961 tggagtgact atgcgaccat cttaacagta gatcaaggcc ctgatagtgc agattattat
     1021 attgggaaca atgctgtaaa gggtgatatt gtggttacac aagattatgg acttgcagct
     1081 atagctttat ctaaagaagc tctctgttta aatcaaaatg gtttaataat ttcttcagga
     1141 aacatcgatt tattactgga tcgcaggtat tttaatcaac aactccgcag aaaacataaa
     1201 aagtacacta aatttaaaaa gaggacaata gaacaagatg agaaatttga gcaaagtctt
     1261 aaacaactga ttcaaaaatc aatgtagttc taaggctact catgcgattg taaaagaata
     1321 taataagggt gtgaggtctt ttatggtgta tcaacaagat tggatgatga ggcaaattga
     1381 aagcatggtt caagggattg caagacttat tttcaaaaaa gatactataa cttatgagct
     1441 tatagatgaa acaaactaca cgaaaacaga tttatattac aaggaactac ttgaattact
     1501 aaattcttca aaaataaacg aagctgaaaa tttattgttt gaacgtatag aatctagcga
     1561 cataaattac ttgacgatag ccgtagattt ttataacaga ttgaataaat taagtgatga
     1621 gcagttagaa aaaagcaatt tttccagggt tgaaattaaa agtggacttg aagatgtttt
     1681 aaaaatattt gaccttatgc cgtagtctat gctacagtta tacaaaatta tggctttgtg
     1741 atggaagatt atggaaaatc aaaaattatg cgtatgaaaa acctataccg gtttaattaa
     1801 taaggagatt attatgattg caagggttaa gaaaaaatca tgtatatgga caatagttgc
     1861 agttattttg gtgctattgc tagcctgggt aggacttaca aaatcaagat atagaacaca
     1921 aacacatgat acgaataatg gggcaaattt tgatgggaaa tctcaatcaa atatactgtc
     1981 agaaaacccc attaatttga ctgataactt acagaaccat aaacttgttg tgaaaatgat
     2041 tgatggagaa tattacgatg atgaagaacc cggtcctttt atgggaccaa actgggttgg
     2101 caaatttgct gtggaactca ttaatgtaaa aggaaatgta atatctgaat tcgaccttaa
     2161 taaatcgttt aatgaggcaa aactacgttt tggtagtact tttgaaatag aatttgatga
     2221 ttataatgat gatggaaatt tagattttac aattggacaa tatgcttcaa gtaatggcag
     2281 agattataag atatttacca tacgggaaga taaaaaaatt gccgaacttc ctattaagga
     2341 ttacccaact ttattcataa gtaatactac aggatattat tctacaaggc taacaaaagt
     2401 tgataaaact acatttaaaa aagaatatta tgataattct gaaagtaaat gctttgagga
     2461 tgttttccaa tggacaggaa aagaatttgt ttggattaat aagagggaag caaatggagc
     2521 ttatgtagat catgttgcta cttttgataa tggtggaaaa attttgtata aaaagaatta
     2581 tggaaacaca gaaattataa tagaaaatga aaagaaagaa acagtattaa acgacaactc
     2641 accatcatat ccaatagtac aacaagacag aaaaaacatt gcatatataa gtccccttga
     2701 gtgggagcaa atagggcaga tttatgtcta tgaccctgaa aagaatacca ataatgcagt
     2761 cctgggcaaa gatgatatac caaaacaata cacaccgaaa aaggtatact ggctcaacga
     2821 aaaatattta cttgcagtaa ttggctttgc ttatggtacg gttgatgttg gtggtgatgt
     2881 ttatgttata gacatagata cggggaatta tgatatttat ttaaaagcag atgagaatca
     2941 gcagattaat gatatatata cagttgcaga taaaatagtt tttttaacag tagaatttaa
     3001 cgagcaagaa aagaaatata tatatggaga cgaaataata attaaacatg atgagctatt
     3061 aaaatttgcc tttagcttga agaaaggaaa agaataagga cggtttctaa acttaccgtc
     3121 cctcatctat atttaatgca atttcttttc tatttgacta taaatctgtt ccgctgtctg
     3181 tcctttggca ttgattactg ttgcatttgt gcttgtatct tcaatcccta agaaattctc
     3241 atttatacct gtaactacta cagcatcata ttcatccagg tctcttgcat actctctact
     3301 aaaatttatg ctgtcgacag aatagccttt ttgtgcaagg aaatctttta cttgcgtaag
     3361 actttcttct actgctattt tgtggttcat aatatccatt ctcctccaat tatttaatat
     3421 tctatctcag ttagtatctg tagattttaa aataataaac tacaaatata tcttacatat
     3481 atgtatatgc aaacatattt aacttgcggg tgtgattttt tcattcgaag aaaaataaac
     3541 caagggaaga atacattacc aa
//
LOCUS       DAOSDD010000357         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9239_length_3558_cov_3.562375, whole genome shotgun sequence.
ACCESSION   DAOSDD010000357 DAOSDD010000000
VERSION     DAOSDD010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9239_length_3558_cov_3.562375"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..845
                     /locus_tag="PK733_18960"
     CDS             138..845
                     /locus_tag="PK733_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12651.1"
                     /translation="MNVSKSKKRYFKRYFYIFILVIVISLLLVHTVSGIGPDPGSDED
                     PLVTKGYVDSELGKFSAQVNSKINLLKSELRKSKSDEDTETVKRKFLELEETVDNLNN
                     QIEEQLKRTKFKVVELEAGQCIILGDSAEIILRAGKALAISGEKGDGLADITTDSKKN
                     NLVTDDIVPLNHLLLASRNDGRGIKAVAKAWVLVKGGYSIEGDNIDEDKKADEEAEEK
                     EGVEDNSNNSNNSNNVD"
     gene            complement(1200..1949)
                     /locus_tag="PK733_18965"
     CDS             complement(1200..1949)
                     /locus_tag="PK733_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOJ12652.1"
                     /translation="MRNTIDILGIPVDNINMEKAMLIIRNFLVDGEVHTIYTPNSEIM
                     MAAQRDKYLKDVLRQGDMLIADGAGVVLASKILGCKLPERVAGFDLVRNIFQISSETK
                     LKFFLLGAKPGVAEEATVSIINRFKGAEISGYHHGYFSDEEEDGIINQINSSGAEILL
                     VALGAPRQEKWIHENKNRLKVKICIGVGGTLDVFAGKVARAPEFFRKHNLEWLYRLHK
                     EPWRFWRMLDIPQFLLLALHYRLFKSKTHNT"
     gene            complement(2071..>3558)
                     /gene="csaB"
                     /locus_tag="PK733_18970"
     CDS             complement(2071..>3558)
                     /gene="csaB"
                     /locus_tag="PK733_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOJ12653.1"
                     /translation="VFFTGWLNDPYELMGIVDISVLTSISESFAYSILEGARFKKATI
                     STNVGGIPDLIESGKNGYLFNPGNYEKLADYILELAENEDMRVIMGENIYKKAKTMFS
                     LEKMCETQLDIYKTILKRSACKKENNLEYDVVIAGYYGFKNIGDEALLMAIIQDLNLY
                     IDDIRIVVLSNNPMETRILYSTYSINRFNLIKIFRAMKKSRMFMYGGGTLIQENTSTR
                     SLIYYLGMIWMAKKTGLKVMLYANGIEPINKYINKRLTKVILNKVDTITLREEASKEE
                     LQKLGINKPATIVTADPAFTIEPCNNNNIEKIFIDEGIDLYSPIMGFSVRKWHGYTNY
                     VKVIARIADYVVEKYHVNPVFLPMQHPDDLLIIKSIVSRMKHKGYILKNKYSVSQVLG
                     ITSKMDILIGMRLHALVFAVSSGIPVVGLVYEPKVQWFLEYIGQGPASAGDVKTLEFE
                     KLKSVIDYTWENREQIKKCLDKVTVELKEKSIKNAQIAIEVLNGF"
BASE COUNT         1142 a          636 c          591 g         1189 t
ORIGIN      
        1 gataatcctg cagtgaaaag ccgtaaaacg gctttctggg gaacaatcat attattgttt
       61 attactaatt atatttaaac attaatgtat tttatttttg gtgatgcaat ttaaaaatta
      121 agggggatat acaaaagatg aatgtttcta aaagtaagaa aaggtatttt aaaagatatt
      181 tttatatttt cattttagtg atagttatca gtttgttatt agtacatact gtcagcggta
      241 ttggtcctga cccgggaagt gatgaagacc ccctggttac caaaggttat gtagattctg
      301 aacttggaaa attttcagcc caagtaaatt ctaaaataaa tttactgaaa tctgaattaa
      361 ggaagtcaaa aagtgatgaa gatacagaaa cagtgaaaag gaaatttctt gaattagagg
      421 agactgtaga caatttgaac aaccagattg aagagcaatt aaaacgtaca aaatttaagg
      481 ttgtagaatt ggaagcaggg caatgtatta tacttggtga tagtgccgag ataatattaa
      541 gagcaggcaa ggctcttgct atatcaggtg agaaaggtga tggtcttgct gatattacaa
      601 ctgatagcaa aaagaacaat ctggttacag acgatatagt acccctaaat cacctgcttt
      661 tggcatcaag gaatgatggc agaggtataa aggctgtagc aaaagcatgg gttcttgtaa
      721 agggcggcta tagtatcgaa ggggacaaca tagatgaaga caagaaggca gatgaagaag
      781 cagaagaaaa agaaggtgta gaagataatt caaataattc aaataattca aataatgtgg
      841 attaatcaga aaagatgtat aacctaaata cctgtatttt tataactatg atctctgaaa
      901 gcattatgat atctataaaa tgtgcaatct agacatagta aaattataac tatttatatt
      961 tgaacagata tcttaaatgc attagtatta ctgtattgca agtttcatag ttataatttt
     1021 taagggaatt taggtattac agctacacat taattcgtga ccagacagaa aaatgctgaa
     1081 acaccttatt ttcttcatat cacaggtatt atatctgata atgaaattcc atggagctgt
     1141 aatatgatgc caaaagatac gatgcggtgg atatgatgca gtagatatag gcaggtatat
     1201 caggtattat gggttttact tttgaataat ctgtagtgta gagcaagtag caaaaattgt
     1261 ggaatatcaa gcatccgcca aaaacgccac ggttctttgt gcaatctgta aagccattca
     1321 agattatgct ttcgaaaaaa ttccggtgcc cgggctacct tccctgcaaa aacatcaaga
     1381 gttcctccaa caccaataca aattttaact ttaagcctgt ttttattttc atgtatccat
     1441 ttttcctgtc tgggtgctcc aagtgccaca agcaatattt ctgccccgct ggaattgatt
     1501 tggttaatta taccatcctc ttcttcatct gaaaaatatc catggtgata tcctgatatt
     1561 tccgcacctt taaacctatt aattatacta actgttgctt cttcggctac cccgggtttt
     1621 gcgcccagaa gaaaaaactt aagctttgtt tctgatgaga tttgaaaaat atttctcacc
     1681 agatcaaatc cagctacgcg ttcaggcaat ttgcaaccaa ggatttttga ggccaacaca
     1741 actccggcac cgtcggctat aagcatatca ccttgcctca aaacatcttt gaggtactta
     1801 tccctttggg cagccatcat tatctctgaa tttggagtat atatagtatg cacctcaccg
     1861 tcaactaaaa aattccttat gattaacata gccttttcca tatttatatt atcaactggt
     1921 attcctaata tatctatggt atttctcata tttattcagg ctcctctcta taataaatat
     1981 agataataaa tatagataat caacactaat tccttgtgaa acttgatttt atcatttttg
     2041 caaccattaa cctattgcat tatatttcca ttaaaacccg tttaggactt caattgcaat
     2101 ttgagcattt tttattgatt tttctttcag ttcaactgta actttatcca agcatttttt
     2161 aatctgttct ctattttccc atgtataatc aataacgctt tttaactttt caaactcaag
     2221 agttttgaca tctcctgctg aagccggacc ttgtcctatg tattcaagaa accactgtac
     2281 ttttggttca tatacaagcc ctactacagg tattccagag cttacagcaa aaacaagtgc
     2341 atgaagccgc ataccaatta aaatatccat ctttgaagtt atccccagaa cctgagatac
     2401 actatacttg tttttcagaa tataaccctt atgcttcatt ctggagacaa tcgacttaat
     2461 tataaggagg tcgtcggggt gctgcattgg taaaaataca ggattaacat gatatttttc
     2521 tactacataa tctgcaatac gggctataac ctttacatag tttgtataac catgccattt
     2581 tctcacagaa aagcccataa ttggactata cagatctata ccttcatcaa tgaaaatctt
     2641 ttcaatgtta ttgttgttgc atggctcaat ggtgaatgcg ggatccgcag taacaatagt
     2701 tgcaggctta tttatcccaa gcttttgcaa ttcttctttg gaagcttctt ctcttaacgt
     2761 aattgtatct accttattta gaatcacttt ggtcagtctt ttattgatat atttatttat
     2821 tggttctatt ccatttgcgt aaagcataac cttaagcccg gtttttttcg ccatccatat
     2881 cattccaaga tagtaaatca gagagcgggt gctggtattt tcctgaataa gagttccgcc
     2941 tccatacata aacatcctgg actttttcat tgccctgaag attttaataa ggttaaagcg
     3001 atttatagaa taggtactgt aaagaatcct tgtttccatg gggttattag aaagcacaac
     3061 tatcctgata tcatcaatat atagattaag atcctggatt atagccatga gcaatgcttc
     3121 atctccaatg tttttaaagc cataatatcc tgcaattaca acatcgtatt caagattatt
     3181 ttcttttttg caggcagatc ttttcaatat agtcttataa atatcaagtt gtgtttcaca
     3241 catcttttcc agcgaaaaca ttgttttggc ttttttatag atattttcac ccattattac
     3301 tctcatatct tcattctctg ccagctcaag aatgtaatca gctaatttct cataatttcc
     3361 gggattaaac aaatagccat ttttgccgct ttcgataagg tcgggaattc ccccaacatt
     3421 agtacttatt gttgcttttt taaatctggc tccttctagg attgaatatg caaagctttc
     3481 actaattgag gtcaacacac ttatatctac aatgcccata agttcatatg gatcgtttag
     3541 ccagcccgtg aagaaaac
//
LOCUS       DAOSDD010000358         3556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9246_length_3556_cov_5.901457, whole genome shotgun sequence.
ACCESSION   DAOSDD010000358 DAOSDD010000000
VERSION     DAOSDD010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_3556_cov_5.901457"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PK733_18975"
     CDS             complement(<1..967)
                     /locus_tag="PK733_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ12654.1"
                     /translation="MKMHSGVDISVKDGRRVVTGLGYYKTREISDLRELVKGTAGING
                     DTVGYKFKDKDGRIVGKTYKEFDSEVDCLGTALMSLGLKDAHISIISENRYEWGVCYF
                     SIINGTGVAVPMDKYLPEKEVENLIERGRVEAIFYSAAFHNIMLEISKKNQRIKYYIC
                     IDDIPDKSDKSDKPDKSDKPDKPDKLGKSDIYDLSNIACKSDKVAQYPGFVTLSSLIS
                     KGRKLIETGDRSFIDASIDPHKMSILLFTSGTTSISKGVMLSHANIVCNVLSIAATIK
                     TYPTDVHLSLLPLHHTFENTVGLTFMIYNSVCIAYCEGIRHIARNI"
     gene            complement(1557..2828)
                     /locus_tag="PK733_18980"
     CDS             complement(1557..2828)
                     /locus_tag="PK733_18980"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ12655.1"
                     /translation="MLVGVNKKLITPKESIDLAGFGTSDRKAVGVHDDLYITTLALQH
                     GDKRILIICGDIIGFGFNFTKRIKEDIHEKFGFNEDEILLNASHTHSGPQTLENMLVS
                     VGEMYHEYMENLHNYVISSAESALNSMDKVDIYYGETQCNICINRRLITDGKANFQPN
                     EKGIADKAVTVLKFMKGNMVKAVLFSYACHPSIVGTKYISSDYPGAAKKVIEDGLGKD
                     TVALFMQGCCGNIRARTVEGNSFRTGTWDDVENFGNELGTKVVDLCNGKMKKASEAGM
                     DISSQIIHLQIPIKDIPEKKKLEAIKKSGSTDEKLWAAKMLDKYSSITDTLPFVVQRI
                     TLSDDISIITMSGEVCIEYGLYVKNKNPDKIIIPVAYSNGVTGYIPTEEMFSEGGYEP
                     YGSTIYYSFPSQLDTSVEKIVVDALDKIIKN"
     gene            3113..3361
                     /locus_tag="PK733_18985"
     CDS             3113..3361
                     /locus_tag="PK733_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12656.1"
                     /translation="MNYRELLELTKKHPNPFSIDHRNTRMYDFEMEEDVQKATDNIKV
                     AIKKTEKGEQQSSQDRQKASDYSASYREVLKTCKRHYY"
BASE COUNT         1088 a          699 c          535 g         1234 t
ORIGIN      
        1 gtatatttcg tgcgatatgc ctgattcctt cacaatatgc aatgcagaca ctattatata
       61 tcataaacgt cagacctact gtgttttcaa aggtgtggtg gagaggaagc agcgaaagat
      121 gcacatctgt aggatatgtc ttgatagtag cggcaatgga taaaacatta cacacaatat
      181 tagcatggga tagcattact cccttggata tactagttgt acctgaagta aaaagtaaaa
      241 tagacatttt atgtggatca atagaagcat cgataaacga gcggtcaccg gtttcaatca
      301 acttcctgcc cttactgata agggaagaca aagtaacaaa gccgggatac tgtgctactt
      361 tgtctgattt gcatgcaata ttagataaat catatatatc cgatttaccc agtttgtctg
      421 gtttgtctgg tttgtctgat ttgtctggtt tgtctgattt gtctgatttg tccggtatat
      481 catctatgca tatataatat tttattcttt ggtttttctt tgagatttct agcattatat
      541 tatggaaagc agcgctgtaa aaaatggctt caactctgcc tctttctatc aggttttcca
      601 cttctttttc aggcagatat ttatccatgg gaacagctac gccagtgccg tttattatgg
      661 aaaaataaca tacgccccat tcatacctgt tctcgctaat aatagaaata tgagcatctt
      721 tgagacctaa tgacattaaa gccgtaccta ggcagtcaac ttcagaatca aattctttgt
      781 atgtctttcc gacaattcgt ccatctttat ccttgaattt atatcccaca gtatctccat
      841 ttatgccggc agttcccttg accaattccc tcaggtcact aatttcccgg gttttgtaat
      901 atcccaaccc tgtaacaact cttcttccat ctttaacgga aatatctaca ccactatgca
      961 ttttcataca tttcccacct ttttccagtt aataaaatat cattcgagat tgtattaact
     1021 gctattatta cctggtaata attatacttt attttcatag tttatgcaat aataatttac
     1081 ttcttcatat ttttcttgta aacaactcct gtcatagcag aatttcgttt gtacgcagta
     1141 ctctttataa ttcagcaatg cccccttcac agacaaccgc ttcatattgc accgtaccca
     1201 taatatgatt gttccccaga aagccgtttt acggcttttc actgcaggat tatctgcagt
     1261 gagttgcaat ttcgttgcaa cctggtgcaa tctaatatgg ttatccagcc ttctttaaga
     1321 aggctgatga aatccgatgt gccttaaatt tcatctgaaa gtcatttcat gactttcagg
     1381 gtgataacca taatattatc tttttcttgc aactaaatac taagtacagc tacacataaa
     1441 ttcgtgacca gatagaaaaa cgctgaaaca ccttattttt ttcacatcac agctattata
     1501 tctgataacg aaattccata gagctgtact aagtatatca cgttgcttgc taggtattaa
     1561 ttcttaatta ttttatctaa agcatccaca acaatttttt ctacagaagt atcaagctgt
     1621 gaaggaaaac tataatatat tgtactacca tacggctcat atcctccttc actgaacatt
     1681 tcttccgttg gtatatatcc tgtaacacca ttactgtatg ctacaggaat aataattttg
     1741 tcaggatttt tattttttac gtataagcca tactcgatac atacttcacc actcattgta
     1801 attattgaga tatcatcact taatgtaatt ctctgaacta caaaaggaag agtatccgta
     1861 atactgctat atttatcaag catttttgca gcccataatt tctcatcggt actgccgctc
     1921 tttttaattg cttccaattt cttcttttca ggtatatcct ttattggtat ctgtaagtgt
     1981 ataatttgtg aagatatatc catgcccgct tcacttgctt ttttcatctt tccgttgcac
     2041 agatctacaa cctttgtgcc caactcattt ccaaaatttt ccacatcatc ccaggtccct
     2101 gttctaaagc tattgccttc tacggttctt gcgcgaatat ttccgcagca cccttgcata
     2161 aacagtgcaa ctgtatcttt gccaagacca tcttctatta cttttttagc tgcccccgga
     2221 taatctgaac ttatatactt agtaccaact atgctgggat ggcatgcata actaaataat
     2281 actgctttta ccatattacc tttcatgaat tttagaactg ttacagcttt gtctgcaata
     2341 cccttttcat taggttgaaa attggctttc ccatcagtaa tcaaccttct gtttatacaa
     2401 atattacatt gtgtttctcc ataatatatg tcaactttat ccatcgagtt tagagcagat
     2461 tctgctgatg atattacata attatgtaaa ttttccatat attcatgata catttcacct
     2521 acggaaacca gcatattttc aagagtctgt ggtccgctgt gggtatggga agcattaagt
     2581 agaatttcgt cttcattaaa cccaaatttt tcatgtatat cttctttaat tcttttagta
     2641 aaattgaatc caaatcctat tatgtcaccg caaatgataa gtattcgctt atcaccgtgt
     2701 tgtaatgcca aagtagttat gtacaaatca tcatgcacac caacagcttt tctgtcggac
     2761 gttccaaacc ctgcaagatc aatactttcc tttggcgtta tcagtttttt atttacacca
     2821 accaacataa tactaatccc ccttaaagat tgtttcttat tattgtttct tatttattat
     2881 attccacttg tgcagccgtt tcaactacca tcggttaaac cgggtgcatt taccatatta
     2941 aagtgtagtt gtctacaaat agcgttattc tcataatatc tcaacatact ttttaatttt
     3001 gtttacataa attcaacaat tcatgatctt tttaatataa tataataggt tacttagcag
     3061 gcctgatcaa ttatgtaaat ttaataaata ttatcaagtg ggaggttgta gtatgaatta
     3121 cagagaatta ctggaactga ctaagaaaca ccctaatcct ttttccattg atcatagaaa
     3181 tacacgtatg tatgactttg agatggaaga ggatgttcaa aaagcaacag ataatatcaa
     3241 ggtagctata aaaaagactg aaaagggaga acagcaaagt tcacaggaca ggcaaaaagc
     3301 atcagattat agtgcttcat atagagaagt tctaaaaaca tgtaagaggc attactactg
     3361 atgagaatgc acatcctatc ataaaggtgg attaatttaa gtgaaaaaat tacggcaaac
     3421 atcaatttct acgtaaaata ttcactgcaa cagcggattg taacatttaa gtctattgaa
     3481 aaattaaata taccatgtca taaggtagtg tcaaataagt aatgaaaact gatgataaac
     3541 ggtaataaaa tgattg
//
LOCUS       DAOSDD010000359         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9266_length_3551_cov_4.539474, whole genome shotgun sequence.
ACCESSION   DAOSDD010000359 DAOSDD010000000
VERSION     DAOSDD010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_3551_cov_4.539474"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..732)
                     /gene="spoVG"
                     /locus_tag="PK733_18990"
     CDS             complement(454..732)
                     /gene="spoVG"
                     /locus_tag="PK733_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOJ12657.1"
                     /translation="MEITDIRIRKIDAEGKMKAVVSVTFDNEFVVHDIKVIESQNGMF
                     IAMPSRKTPNGEFKDIAHPINAATREKIQKAILEKFDAAAGESQETND"
     gene            1117..2499
                     /gene="murC"
                     /locus_tag="PK733_18995"
     CDS             1117..2499
                     /gene="murC"
                     /locus_tag="PK733_18995"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ12658.1"
                     /translation="MQKKLLDSDNIRYIHFIGIGGISMSGLAEILINLGYIVSGSDLK
                     KSGITHKLENLGADIFYGHSPDNIRNPDLVVYTAAIKENNPELVRARSLNIPVIDRAA
                     LLGLIMKKYPYSVAVSGTHGKTTTTSMISMIMLESGLDPTVHIGGELDYIGGTTKTGN
                     TEYFVAEACEYCDSFLKFFPYIAVILNIELDHVDYFRDIEHIKKSFISFATLVPENGY
                     IVACADDNNVLSILDKISAPKITYGINNEDCMWKAKDVSFRISGYGSFTLVKDEKEID
                     LIELKVPGIHNIYNSLAAIAVCYQLGCPISGIKQGLGKFTGTHRRFEQKGILNGIKIV
                     DDYAHHPSEIRATLKAAKNGHYSKIWCIFQPHTYTRTKTLLDDFAISFANADAVIVAD
                     IYAAREQDNGEINSQILVEKINSAGGKALYMKDFDTIAGYLRDNASPGDLVITMGAGD
                     IYRVGEKMLG"
     gene            2531..2671
                     /locus_tag="PK733_19000"
     CDS             2531..2671
                     /locus_tag="PK733_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12659.1"
                     /translation="MGTDTWAIHGDSPLVSKGFFAETGSETKIREKTEDKQKRKDSSY
                     VS"
     gene            complement(2761..3195)
                     /gene="fabZ"
                     /locus_tag="PK733_19005"
     CDS             complement(2761..3195)
                     /gene="fabZ"
                     /locus_tag="PK733_19005"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOJ12660.1"
                     /translation="MLSNIEVQKIIPHRYPFLLVDKVLEIEPGKRAVAIKNVTINEPF
                     FQGHFPGYPIMPGVLIVEALAQTAGIAAASLEENKGRLGVFTGIDSMKFRKQVVPGDV
                     LRLEAEILVLKMGMAKVKVSATVDGQTAAEGEIKFAMIDAGK"
BASE COUNT         1078 a          622 c          644 g         1207 t
ORIGIN      
        1 agaattacct ctgttcgagt actatgagta ctatgttttt ctatcacgag ttatcctcct
       61 ccaataaata ttttctcact tttcggtgtt tttttcaacc tgatcgtcta tttttgcaca
      121 caaaaaaaca gcctacaggt aacttgcagg cttgtaggct aatgcaacaa tacgtgaacc
      181 tcacataatt aaagtcacgt gcttcaaaca tcactgttga aattccctgc ttcttggact
      241 ttgtaacctc cgtctccaca ggcttataat cccgtagtcc ttacggcatc atttgtttat
      301 actttgtgcg gcattaagca taccagcaag cagttgaaac tccgcatgga taaagccggc
      361 agacgaggta ggggctatct tgccgatcat tgtaatacca acagcttttt ctatgtactg
      421 agccgggaat tatattatct gttcatcagt cagttaatca tttgtctcct gactttcacc
      481 ggctgctgca tcaaactttt cgagaatagc tttttggatc ttttcccgag tagcagcgtt
      541 tatggggtgt gcgatgtctt tgaattcacc attgggagtt tttctgcttg gcatcgctat
      601 gaacataccg ttctggcttt caattacttt aatatcatga acaacaaatt cgttgtcgaa
      661 agtaactgat accacagcct tcattttacc ttctgcatca atttttctga tacgaatgtc
      721 agtaatttcc atggatgtta ccaccttccg tttaacatta taatgtataa tgtatgtatt
      781 cgctacatat ttgatttttc ctgctttttt tccatatatt ttttattttt atttgttttt
      841 gttattttta taggttttca acataatttt taccgcatat tctataattg agaataaaat
      901 atgaagtttt agatgtataa ttatggtgca taattatatt agtgttgtat aataaatgta
      961 tgttgtattt ataagttaat aattgatgtt tgtattactt tacttatgga taacttatga
     1021 caatatgaca aattaaactg aaagatgtag ctttaaatta atgaagcagt attaatataa
     1081 atttaagcaa tgtaaagtaa ggagtgtaag tttggtatgc aaaaaaaact tttagattca
     1141 gataatatta gatatatcca tttcataggt ataggcggaa taagtatgag cgggttggca
     1201 gaaatactta tcaatcttgg atatatcgtt tccggttcag atcttaaaaa gtcgggtata
     1261 acacataagc ttgagaatct gggggctgac attttctacg gtcacagtcc tgataacatc
     1321 agaaatcctg atcttgtagt ttatactgct gcaataaaag aaaataatcc cgaattggta
     1381 agagccaggt cattaaatat acctgtaatt gatagagctg ctttgcttgg tttaataatg
     1441 aaaaaatacc cctacagtgt tgcggtgtca ggtactcacg gcaaaactac tacaacttcg
     1501 atgatatcaa tgattatgct tgaatcaggc cttgatccta cagtacatat aggcggagaa
     1561 ttggattaca ttggtggtac cactaaaaca ggcaatacgg aatattttgt agcagaagcc
     1621 tgtgaatact gtgacagctt tctaaaattc ttcccctata ttgctgtaat tcttaatatt
     1681 gaacttgatc atgttgatta tttcagagat atcgaacaca taaaaaaatc ctttataagc
     1741 tttgccactc ttgtacctga aaacggttat atagttgcat gtgctgatga taataatgtt
     1801 ttatccatac ttgataaaat cagtgcacct aaaataacct acgggataaa taatgaagat
     1861 tgtatgtgga aagcaaaaga tgttagcttt agaatatcag gatacggttc ttttactctt
     1921 gttaaagatg aaaaggaaat tgatttaatt gaacttaaag tgccaggtat tcataatatt
     1981 tataactccc ttgcagctat agctgtctgc tatcagcttg gttgccccat ttccggaatt
     2041 aagcagggat taggtaaatt cacagggact cacaggcgtt ttgaacaaaa gggaatttta
     2101 aacggaataa aaatagttga tgattacgcc catcacccat cagagataag agccacacta
     2161 aaagctgcaa aaaacgggca ttactctaaa atatggtgta tatttcaacc tcatacctat
     2221 acccggacaa aaactttgct tgatgatttt gctatttcat ttgcaaatgc tgatgctgtt
     2281 attgttgcag atatttatgc tgccagagaa caggataacg gtgaaatcaa ctctcaaatt
     2341 cttgtagaaa agataaattc tgctggaggc aaagctttgt atatgaaaga ctttgatact
     2401 atagcaggat atttaagaga taatgcatct ccgggcgacc ttgtaataac aatgggcgca
     2461 ggagatatat atagggtcgg agaaaaaatg ctgggctgaa acaaggacct gaaacacaag
     2521 gatcctacat atggggaccg atacatgggc gatacatggg gacagtcctc ttgtttcaaa
     2581 aggtttcttc gcggaaacag ggtcagaaac gaagataaga gagaaaacag aagacaagca
     2641 gaaaagaaag gacagttctt atgtttctta gaatgaaata gagaaccgtc ctattgtttt
     2701 ttattgtttt gttcgtttct tttgtttata tttattccaa attttccgtt tattttccgt
     2761 ttatttccca gcatcaatca tggcaaattt gatttcacct tcggcagctg tttgtccatc
     2821 tactgttgca gatactttga cctttgccat acccatcttt aaaacaagga tttccgcttc
     2881 cagccttaaa acatcaccag gaacaacctg ctttctgaat ttcatggaat ctatcccggt
     2941 aaatacacca agtcttcctt tattctcctc aaggcttgca gcagctattc ctgcggtctg
     3001 agcaagagct tctacaatta agactccggg cataataggg tacccgggga agtgtccctg
     3061 gaaaaatggt tcatttattg taacattttt tattgcaact gctcttttac ccggctcaat
     3121 ttccagtacc ttatcaacca gcaggaaagg gtatctgtgg ggtattattt tctgtacttc
     3181 aatatttgat aacattttgc acctctcttc agcgtctaaa acgcagtata aattttataa
     3241 gctgtacaat attttagatt aactttttaa tatttgcaac ttagtactac tttgttacat
     3301 tgtcaatgga aatcgcattc caaattggca tagtagcatt aggttattat tttattattc
     3361 tatttattct acatctgtta tgaaaaatcc ttttatgtaa aaatttccag cgaatttatt
     3421 agattttttt gcaaaatata tttgattttt taaaacattg gtgctataat tagttttggc
     3481 tgttggattt ttagtttaag ggcgcttagc tcagctggga gagcacctgc cttacaagca
     3541 gggggtcata g
//
LOCUS       DAOSDD010000360         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9279_length_3548_cov_5.487547, whole genome shotgun sequence.
ACCESSION   DAOSDD010000360 DAOSDD010000000
VERSION     DAOSDD010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_3548_cov_5.487547"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PK733_19010"
     CDS             <1..1021
                     /locus_tag="PK733_19010"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ12661.1"
                     /translation="AHFDVEDYWFYKTYIPYTTIRPKEERNEMLLSDMKKHGLFVVSG
                     DISGWSDEFLTMFGLAVFLTTPTEIRMKRIENREYARWGDRVCEGGDMYESQKKFREF
                     AATRDVGLLEQRALEYSCPVVYYDGTKTPGEIVDEVLTYINTRNHYDALIDENNDPVY
                     DPAPLKAHMDKWDGEAFIEALLLSPDKSVLEIGVGTGRLAMRVCDKCRHFTSIDISPK
                     TVQRAKANLQRFTNTDFIYGDFLTYPFGDRFDIIYTSLTFMHIQDKRAAIRRVASLLK
                     PSGRFVLSIDNNQQAEIYYGNRRIIVYPDTPEETTALIVEAGLTIEKQFETEFAVIFV
                     ATKEV"
     gene            1025..1681
                     /locus_tag="PK733_19015"
     CDS             1025..1681
                     /locus_tag="PK733_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12662.1"
                     /translation="MNCPECGSNLLHCHKLIDGTVSPMKNRSNIIYPNCIDCGHPIQV
                     EDVEANITGNRKVLLISGTAGAGKTALGQYIESKSDYIFIDGDAVSKRVNHYARLNPS
                     AAVPDYQKETIRTMLVLLGLGYDVVVGYVINQEVLRWYHKGLERYQITPVFRVLVPDR
                     DVCLQRDIDRECWTAGAEYVDRWFDEQRAYLKTAPEICIDSSQETLADTFANHFQGLL
                     "
     gene            1712..2323
                     /locus_tag="PK733_19020"
     CDS             1712..2323
                     /locus_tag="PK733_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOJ12663.1"
                     /translation="MATWLTHLRVAERVSDQINITDRSLFFAGSIAPDSDTLSDISHW
                     CSNADKITCDAQGFYINYISDSLHTEDKDFYWGYYIHLLTDILWHKQKIAPLKHESKD
                     TIRAIKQKWRNVDISFLADYRDFKPLTEMKYAMKCTSKYDKRWLDYYIAGQVRKLVEG
                     ILGNLIISEIEKQSRDTATETEIEQFIDECVEYIGLKLKEGAK"
     misc_binding    2601..2813
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2897..3487
                     /gene="argS"
                     /locus_tag="PK733_19025"
                     /pseudo
     CDS             2897..3487
                     /gene="argS"
                     /locus_tag="PK733_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
BASE COUNT         1120 a          615 c          840 g          973 t
ORIGIN      
        1 tgcccatttt gatgtggagg attactggtt ttataaaacc tatataccgt acaccaccat
       61 tcgaccaaag gaagaacgga atgaaatgct gctttcagat atgaaaaaac acggcttatt
      121 cgtggtatcc ggcgatattt caggctggag cgatgaattt ctaacgatgt tcggcctcgc
      181 agtctttctt acaacaccta cggagattcg catgaaacgc atcgaaaaca gggaatatgc
      241 acggtggggt gaccgagtat gcgaaggcgg cgatatgtat gagtcgcaga aaaaatttcg
      301 ggagtttgcc gccacaaggg atgttggttt gctcgaacaa agggctttag aatattcatg
      361 ccctgtcgtt tattatgacg gcacgaaaac gccgggagaa attgtagacg aggtattgac
      421 ctatatcaat acccgcaacc actatgatgc ccttatcgat gaaaacaacg acccagtgta
      481 tgaccccgct ccgctaaaag ctcacatgga taaatgggac ggagaggcat tcattgaagc
      541 gttactcctt tcgccggaca agtctgtttt ggaaatcggc gttggcacag gacgactggc
      601 gatgcgggtt tgtgataaat gcagacactt caccagcatt gatatttcac caaaaaccgt
      661 gcagcgggcg aaagcgaatc tacagaggtt tacgaacacc gactttattt acggcgactt
      721 tcttacttac ccatttggtg atagatttga tataatctac acatcattaa catttatgca
      781 cattcaagat aagcgagcgg caattcgaag ggttgcaagc ctgctgaagc ccagcggacg
      841 gtttgtctta tcgattgata ataatcagca agctgagatt tattatggta accggcggat
      901 tatagtctac cctgacacgc cggaggaaac caccgcttta attgttgagg ctggtctgac
      961 catagaaaaa cagtttgaaa cagagtttgc tgtgattttt gtggcgacga aggaggtttg
     1021 agatatgaat tgccccgaat gtggctccaa cttactacac tgtcataaac tcattgacgg
     1081 cactgtatcg ccaatgaaga atagatctaa catcatttac cccaattgta ttgactgtgg
     1141 ccatccaata caggttgagg atgtagaagc caatatcact ggcaacagaa aagtcctgtt
     1201 aatcagcgga acggcaggag caggtaaaac agctttgggg caatatatcg aaagcaaatc
     1261 tgattacata tttattgatg gagatgctgt tagtaagagg gtgaatcatt acgcgaggct
     1321 caacccaagt gctgcggtgc cggattatca aaaggaaacg ataagaacca tgctggttct
     1381 ccttggtttg ggttacgacg ttgtggtggg ctatgtcatc aatcaggaag tcctaagatg
     1441 gtatcacaag ggattggaaa ggtatcaaat aactccggta tttcgcgtac tcgttccaga
     1501 tagagatgtc tgccttcaga gagacattga cagggagtgc tggactgccg gggctgaata
     1561 tgtggatagg tggtttgacg agcaacgggc atatttgaaa acagccccag aaatctgtat
     1621 tgacagttca caggaaacct tggcggacac tttcgctaat cattttcagg gtttactata
     1681 gagccgtcta tggaggaggt gcgatggacg tatggcgact tggcttactc atctgcgtgt
     1741 tgccgaaaga gtgtctgacc aaataaatat tactgacaga agtttatttt ttgcaggcag
     1801 tattgcgccg gattcagata cgctttcgga catatcacat tggtgtagca atgccgataa
     1861 aataacttgt gatgctcaag ggttttatat aaactatatc tcagatagcc ttcataccga
     1921 agataaggat ttttattggg gatattatat tcacctgttg acggatatct tatggcataa
     1981 gcaaaaaatc gcacctttaa aacatgaaag taaagatacc ataagggcaa ttaagcagaa
     2041 atggagaaat gtcgatatta gctttcttgc cgattataga gacttcaagc cacttacgga
     2101 aatgaaatat gctatgaaat gcacaagcaa atatgataag cgttggcttg attactatat
     2161 agctggacaa gttagaaagc tggttgaggg tatcctcggt aatcttatta tttcggaaat
     2221 tgaaaaacaa agcagggaca ctgctacgga aacagaaatt gagcagttta tcgacgaatg
     2281 tgtagagtat attgggctaa agctgaaaga aggtgcaaaa tgaaaaattt attattcata
     2341 tgcggtccta acggcatcgg gaaaacaaca atctgtaaag aaatcgtacg aatgttgcct
     2401 aattctgctt acgttgactc tgatctctgt cggcttatga atctgtttat tcttaacgac
     2461 gaaaccattc cgacaatagc aaaaaacata agtgatttac tcatcaattg catggattgt
     2521 acggtcattg aaaccgtgat attttcatat ggtttccatg ggagacggag agaggttttt
     2581 gaaatagtaa tgaaggcagt atcagagtat gaagagggaa gtatccttat aagatttcta
     2641 aaagcgagta ggagtagagt gaaagtccta caagatatta tttggagaaa agggcctcgg
     2701 agaagctacc tgagctttat atgttagggt gtgacgttaa ctgcgttaaa gttatgagtg
     2761 gattgtggat acaatcaact agagtggtac cacgggattt atccgtctct tatttaaaga
     2821 taaggggcgg tttttatttt aagtattttg caaggaggta atgagtaatg aaaaagttag
     2881 ctgaattgat tactaatata gtacaactga tttagcaaca atcattgaaa gaatggaatt
     2941 atataatcct gatgagatta tatatattgt tgataaaaga caggagcttc attttgagca
     3001 ggtattcagg tgtgcaaaga aaacaaatct ggttccaccg gacactaaac taacttttct
     3061 agggtttggc acaatgaatg gtaaggacgg taaacctttt aaaacgcggg atggcggagt
     3121 aatgcggtta gaacacctga taaaggaggc tagtgataaa gtttaccaaa gagttaccga
     3181 gaataaagag attccacaag aggaagcaaa agaaatctca agaaaagtgg ggttagcagc
     3241 tctaaaatat agcgatttat ctaatcagat ttcaaaagat tatatgtttg atgtggatag
     3301 gtttacgtct tctgagggaa atacagggcc atatattata tatactgtag taagaataaa
     3361 ttctatactc aataaggttt tcaaagttaa tcctgggtat agagcagaag cagcttctgt
     3421 gcagctacct tacagtgaaa atgaacgcaa tttgatgcta aaaataactc agtttaatga
     3481 agttattgtg acatcctcac ctcaataaat tgaggtgctt ccatgtggaa gttcggtggt
     3541 tcctatct
//
LOCUS       DAOSDD010000361         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9284_length_3547_cov_3.702176, whole genome shotgun sequence.
ACCESSION   DAOSDD010000361 DAOSDD010000000
VERSION     DAOSDD010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9284_length_3547_cov_3.702176"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..1405)
                     /locus_tag="PK733_19030"
     CDS             complement(536..1405)
                     /locus_tag="PK733_19030"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12664.1"
                     /translation="MLIDVHGHIGRIVPDRREYIDVTNLIAKMDAWGVDRTCILPLSE
                     HPEGAYLECNTEDVITACSRYPDRLIPFCLIDPRYGNHPDMDFTHLLEEYKARGCKGL
                     GEFLPKLDFDDPLCMNLYRQAGKFEMPVLFDMQDRLYGYGVRDDYGLPKLEHALQECP
                     DTIFIGHGPTFWAELSADVPADNRAGYPKGPIKPGGAVPRLMRKHPNLWADISAGSGY
                     NALTRDPAFGLEFLDEFQDKLMFGTDSCLRSEVNIVYPNVAFIRDLRQNKKLSDTVID
                     KIEWKNSTRLLNL"
     gene            complement(1790..2872)
                     /locus_tag="PK733_19035"
     CDS             complement(1790..2872)
                     /locus_tag="PK733_19035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ12665.1"
                     /translation="MSSDKKKEIITIANASDEHPRHSEASVIELKDGKLMIAWMEYLE
                     SDIGSGDNAPNRISVMISDDEGLTWREHRVIIETKEGSVNSYSPNFIRLSEEEIILIY
                     FNYLVLEAGKPPATRAFLKRTCDEGKTFTESEAIWDSMPINFASSVAKKLSNGRVIIP
                     VAKQTGNIWSSTDHFDVGCAFSDDEGKTWEFSDNWIYLPMRGCSEGHIEELRNGRILM
                     VLRTQLGSVFKSYSDDGGKTWSKAQTMGMKAPESCPDLNRIPQTGDLMLTWNNSLYDP
                     RFISHYGKRTPLTVAISKDEGETWTNIKDIEDNPYWGYSNPACTFTTSGKGILTYWAT
                     KYKSDWRMGGPISLKAAIFDIGWLYS"
     gene            complement(2952..>3547)
                     /locus_tag="PK733_19040"
     CDS             complement(2952..>3547)
                     /locus_tag="PK733_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12666.1"
                     /translation="DKGHLYLLIDTGNVFKNGKIQYIREGPLKDINGNVIKIHNRARA
                     CGIDMNMDNIEDLIVGGITYQKGFNTDPEPGAEIYCLINKGLDGNGIPILVPERPFEI
                     IGHNFKFRLNSHVHIQTVDIDKDGKKEAILSSQEENFKGLVFKSAADKIALQYTGMYI
                     DNFYVHDYLLDIDDDGELELVFAGGETGVGTYRKMER"
BASE COUNT         1052 a          759 c          517 g         1219 t
ORIGIN      
        1 aactacacta taaaagtttt gtgcgcttat attaccttaa ttaatacgca aaaatccaat
       61 ttgttccttg taaaatcatt ttagcgttat atctagttaa taaagctcat cttttcatgt
      121 tcaaccttgt aaaaagctgt ttcaacaccc tggctggact ttaaattctc ctcttcttta
      181 ctggaaaagc tgtcttttat gttttcagta ttaatcctgt atcttgaagt aaatggaggt
      241 tttttctttt gctctcctca agtatatttc taataagcct ttccacaaat cctctctccc
      301 taatgtttat atgcacaaaa tatttgtatg caaaaagcag ttaaaatcat gaataaatga
      361 ataaaaaaat aatgccaata taagtattga cactatttta tcgtatttat gaaatttcca
      421 caaccgttga ttatttatcg cttatcactt atcggacatt cattttctaa tgaaacataa
      481 aacgaaactc tagaaccgtc tccatgtttc agtctctatg tttctaccta tgttcctaaa
      541 ggttaagtaa acgtgtactg ttcttccatt caattttatc tattacagta tcactgagtt
      601 ttttattttg ccgcagatcc ctgataaaag caacgttggg atatactata tttacttcac
      661 tgcgcaggca cgaatcagta ccaaacatta acttgtcttg gaactcatca aggaattcca
      721 gtccaaatgc cggatccctg gttaaagcat tatacccgct ccctgctgaa atatccgccc
      781 aaaggttggg atgcttcctc atcaatcgag gaacggcacc accaggctta ataggtcctt
      841 taggataacc tgcacggttg tcggcaggta catctgctga tagttccgcc caaaaggtag
      901 gaccatgacc tataaaaata gtatccgggc actcctgaag agcatgttca agcttgggaa
      961 gtccataatc gtcccgaaca ccataaccat agagacgatc ttgcatatca aatagtacag
     1021 gcatttcgaa tttacctgcc tggcggtaca aattcatgca taaaggatcg tcgaaatcca
     1081 gtttgggaag aaattcacca agtcctttgc agcctcttgc cttatattcc tctaggaggt
     1141 gtgtaaaatc catatccgga tgattgccgt aacgtggatc tatcaggcaa aaaggaatta
     1201 gtctgtcagg ataacgagaa cacgcagtaa taacatcttc agtattgcac tccagataag
     1261 ctccttctgg atgctcgctc aacggtaaaa tgcaggttct atctaccccc caggcatcca
     1321 tctttgcaat tagatttgtt acatcaatat actccctccg gtccggcact atacgcccta
     1381 tgtgaccatg tacatcaatt aacatgttat atcccctttc tttctggcaa acatcgctgt
     1441 ttgacaaatt tatttgtttt tatctgtaca atacaggtaa taaatgtagg taatatatta
     1501 ttttcgatat tatgttttaa atatttaaac tagtttaagt atatattttt ttggtcaaat
     1561 tggcaattct aagttaaaag taagagcaca ttaattttct tagatacata atatgtcaga
     1621 tttgatttta aaaatatagt aatttgaaaa agcccacgga ttgttatttt ttaaagtaaa
     1681 gtaaggtaga ttttttccgt agacttaaat attacagttt ttagagccta taatctcaag
     1741 tatattttat cctgcaaagc accaataaca aatctagaca acaccattct tatgaatata
     1801 accaacctat atcaaatatt gctgccttta agctaatagg tcctcccata cgccagtcac
     1861 ttttatattt cgttgcccaa tatgtcaata tgcctttccc tgatgttgta aatgtgcatg
     1921 ccggattgga gtatccccaa taaggattat cctcaatatc ttttatgttg gtccaagttt
     1981 ctccttcgtc cttggaaata gccactgtta atggtgtcct ttttccataa tggctgataa
     2041 accgagggtc atataagcta ttattccagg ttaacattaa atctccggtt tgaggtattc
     2101 tatttaaatc agggcaagat tcaggtgctt tcatacccat agtttgagct tttgaccaag
     2161 ttttcccacc gtcatcagag tatgatttaa aaactgaacc aagttgtgtt cgcaacacca
     2221 ttaaaatccg tccattcctt aattcctcaa tatgtccttc gctgcaacct ctcataggaa
     2281 gatatatcca attatcagaa aattcccatg ttttcccttc gtcatcacta aaagcacatc
     2341 ctacatcaaa atgatccgtt gagctccata tattacctgt ctgttttgcc acagggataa
     2401 taaccctccc attcgaaagt ttttttgcca cagaactagc aaagtttatt ggcatactat
     2461 cccaaattgc ttcagattct gtaaaagttt taccttcatc gcaagtcctt tttagaaatg
     2521 ccctagtggc aggtggctta ccagcttcga gaactaaata attgaaatat atcaatatta
     2581 tttcttcttc agataatcta atgaaatttg ggctatatga attaacactt ccctcttttg
     2641 tttcaataat aaccctatgt tctctccagg taagcccctc atcgtctgaa atcattaccg
     2701 atattctatt gggagcatta tctccagacc cgatatcact ttctaaatat tccatccaag
     2761 caatcatgag cttcccatct tttagctcaa tcacagatgc ctcggaatgt cgaggatgct
     2821 catcagatgc attagctata gttattattt cttttttctt atcactagac ataatattac
     2881 cccctgtctc tgttttttgt ttcttttttt atattgatgt tcagtttaac tagagcatca
     2941 atatacccct cttatctctc cattttcctg taagttccaa ctcctgtttc cccaccggca
     3001 aaaacaagtt ccaattctcc atcatcatca atatccagca aataatcatg tacataaaaa
     3061 ttatcaatat acattccagt atattgcagt gcaatcttat ctgcagcaga tttgaatact
     3121 aaccctttga aattctcttc ctgactggac agtatagctt ccttttttcc gtccttatca
     3181 atgtcaacag tttgaatatg tacatgagaa ttgagtctga atttaaaatt atgccctatt
     3241 atttcaaaag gtcgttccgg aaccaatatt ggaatgccat taccatcaag acctttattt
     3301 attaagcagt aaatttctgc tcccggttca ggatcagtat tgaatccctt ttgatatgtg
     3361 ataccaccta ctataagatc ttcaatatta tccatattca tatctattcc acaagctctt
     3421 gctctgttgt gaattttaat tacatttcca ttaatatctt tcaagggtcc ttctcttata
     3481 tactgaatct tcccattttt aaatacattg ccggtgtcta ttaaaagata cagatgccct
     3541 ttatctg
//
LOCUS       DAOSDD010000362         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9489_length_3491_cov_3.660070, whole genome shotgun sequence.
ACCESSION   DAOSDD010000362 DAOSDD010000000
VERSION     DAOSDD010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9489_length_3491_cov_3.660070"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK733_19045"
     CDS             <1..209
                     /locus_tag="PK733_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12667.1"
                     /translation="SSLKLNTPGFDGCEFSVYDQKIWYYSISKSSKSKKLLTAPMNFI
                     FCRLLSQQAKDKKGALNIFCFLSI"
     gene            complement(245..1738)
                     /gene="pepV"
                     /locus_tag="PK733_19050"
     CDS             complement(245..1738)
                     /gene="pepV"
                     /locus_tag="PK733_19050"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HOJ12668.1"
                     /translation="MIIGNEILNYREDIIRSLCELIKIKSVADTPREGKPFGEGVNRA
                     LEYVLSLGESLGLKTKNVDGYAGHVEYGEGEEITGVLVHLDIVPEGTGWTIPPFEGIV
                     NEGKIYGRGASDNKGPAIAAVYGLKALKDSGIIPKRKIRVILGTNEETGMQDMQYYFS
                     KEPVPDLGFSPDAMYPIINREKGILHLALIKNEHDKGKNIYGDKYNGSCNDNFKKGCK
                     YAHEFPIWKIWGGEAVNMVPDECRAQIPAEFIKLKDVVYLQELAKKNSGENILISFDT
                     NNGLNILAKGKSAHGASPQSGVNAVYGLLDFLHNAENSMKSNGNEENAAFSYMDNYLK
                     FIYEKKIGSDTTGHSLGIDLKDEESGALTLNLGKIDYNQAEKRVLLDIRYPVTCKYEG
                     IVETIKEQAGKEGIQVEVVGHSLPLYVSATHPLITKLSNAYEKITGEKANLLSMGGGT
                     YARTLKNNGVAFGGVGEGAHQPDENVSIEDLMRHAEICTQAIYEIAT"
     gene            2261..3184
                     /locus_tag="PK733_19055"
     CDS             2261..3184
                     /locus_tag="PK733_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOJ12669.1"
                     /translation="MKHLFIINPIAGKGKALEYIPKIEEYFSRMKDLFFIEVTKYPGH
                     AIELVKQYVDKETYRVYSVGGDGTLNEVLNGMAGSDSSLAIIPAGSGNDFIKSIRNYS
                     VDKESPEKNIMKITSKVSLLERLVKGTESFIDIGKINNRYFINISSLGFDAEVVYKSH
                     KIKKLPLITGLLAYVLSVFATLVSYQSNPLKIKIDGQTIEKGTLLVAVANGRYYGGGM
                     QAAPSAELNDGFLDICLIEYAKRLRILKLFPKFIKGQHAGIKEVSFHRGKNISIISKN
                     KVAMNVDGETNIVEGKVDFEIIPNAVRIVLP"
BASE COUNT         1064 a          713 c          554 g         1160 t
ORIGIN      
        1 tttcttcact taaattaaat acacccggtt tcgatgggtg cgaattttct gtatatgacc
       61 aaaaaatatg gtattattcg attagtaaaa gtagcaaaag caaaaaatta ttaaccgctc
      121 ctatgaattt tatcttttgc cggttgctat cgcaacaggc aaaagataaa aaaggagcac
      181 taaacatttt ttgcttttta agtatatgat ttgaacagag cttatgggtt gttatgtttt
      241 cactctatgt agctatctca taaattgctt gagtacatat ttccgcatga cgcatcagat
      301 cctctataga aacattctca tcaggctgat gggcaccttc tcccacccct ccaaaagcta
      361 ctccattatt ttttagtgtc ctcgcatatg tccctcctcc catagaaaga agatttgcct
      421 tttctcctgt tattttctca taagcattgc ttaatttagt tataagagga tgggttgcag
      481 aaacatataa aggaagcgaa tgacctacaa cttctacctg tattccttct ttcccggctt
      541 gctcctttat agtttcaact attccttcat acttgcaggt aacaggatac cttatatcca
      601 gtaataccct cttttctgcc tgattataat ctatcttacc cagatttaat gttaaagctc
      661 ccgattcttc gtcttttaaa tcgataccta atgagtgtcc tgtagtatcg gaacctattt
      721 tcttctcata aataaactta agataattgt ccatataaga aaaagcagca ttttcttcat
      781 taccgttgct tttcatacta ttttccgcat tatgcaaaaa gtccagtaaa ccgtaaacag
      841 cattaactcc gctttgaggg cttgctccgt gagccgattt accttttgcg agtatattaa
      901 gcccattatt tgtatcaaaa gatattaata tgttttcccc actgtttttt ttggccaact
      961 cctgtaaata tacaacatct tttaacttaa taaattcagc aggaatttgt gctctgcact
     1021 catctggtac catatttaca gcttccccgc cccaaatttt ccatattgga aattcatgtg
     1081 catacttgca acctttttta aaattatcgt tgcagctacc attatacttg tccccatata
     1141 tatttttacc cttatcatgt tcgtttttta tcagtgccaa atgtaaaatt cctttttctc
     1201 tattaattat tggatacatt gcatcagggg aaaaccccaa atcaggtaca ggctccttcg
     1261 aaaaatagta ttgcatgtcc tgcattccgg tttcctcatt ggtacctaat ataactctta
     1321 tttttctttt cggaattatt cctgaatctt ttaaggcttt cagcccgtaa actgctgcta
     1381 tagctggtcc tttattgtcc gatgcacctc tgccgtaaat tttcccttca ttaactattc
     1441 cctcaaaagg cggaatagtc catccagtac cttcaggtac aatatccaga tgaacaagca
     1501 cacctgtaat ttcctctcct tcgccatatt caacatgacc tgcatatccg tcaacatttt
     1561 ttgttttcag acccaaactc tcaccaaggg aaagcacata ttccagagcc cggttaacac
     1621 cttcaccaaa tggtttgccc tcacgcggag tatcagcaac actcttgatt tttatgagtt
     1681 cacagagact tctaataata tcttccctat aatttagtat ttcattacca attatcaacc
     1741 ttttataacg cctcctcttt ttaagttaat ttaaacagct agcattacag gtatttagag
     1801 cattcaaaat atggcattta agccacttgc cacttaccca gacaacttca ttttatcagt
     1861 catattagca aaatacaagt ctttagcaaa cacagggggg tgaatatttt aagagtgaag
     1921 aaatatttac caagagctga cgttacactg cctaggagtc tgctcggaaa agcgaactcg
     1981 tgcttcatac taatattttc ttcacttaaa ttatatgcaa ccaacacagg ggcacatttt
     2041 ctacaaacag aaaatacttc cgaaaatgcg cccgaaaatg cttatctatc ttgcaacaaa
     2101 accgaaactg gtgtagaatt ttactcatag gcttgagcat gagtgctaaa tctatttttg
     2161 tatcaatcaa cttatcatgt ttaacataat acgccgagct tattatagat gcaatattga
     2221 tattctttat atacattgtg aagggatagg tcagtcaatt atgaaacact tatttataat
     2281 aaatccgatt gcaggtaaag gtaaggcatt agaatatatt cctaaaatag aagaatactt
     2341 ttcaagaatg aaagatttat tctttataga agttacaaaa tatcccgggc atgctattga
     2401 gctggttaaa cagtatgtag ataaggaaac ttacagggtt tattccgtag gaggagatgg
     2461 tactctaaac gaagtgctca atgggatggc tggaagcgat agttcccttg ctattatacc
     2521 tgcggggtca ggtaacgatt ttatcaagag tattaggaat tattccgttg ataaagaatc
     2581 gccggaaaag aatatcatga aaattacctc aaaagtctct ttgctggaaa gattggttaa
     2641 agggactgag tcttttatag atatcggcaa aataaacaac cgatacttta taaatatttc
     2701 ttcattaggt tttgatgcag aagttgttta taagtcccat aaaataaaaa agctcccctt
     2761 aataaccggg ctactagcct atgttttaag cgtttttgca acactggtaa gttaccagag
     2821 taatccctta aaaataaaaa ttgatgggca aactatagaa aaaggtactc ttctggtagc
     2881 tgttgccaat ggaagatatt atggcggtgg aatgcaggct gcaccttctg ctgaactaaa
     2941 cgacggtttt ttagatattt gcctgattga atacgcaaaa agactaagaa ttttaaagct
     3001 tttccccaag tttataaagg gtcagcatgc aggcataaaa gaggtttcct ttcaccgggg
     3061 taaaaatata agtattataa gtaaaaacaa ggttgcaatg aatgttgacg gtgagaccaa
     3121 tattgttgaa gggaaggtag attttgaaat aatacctaat gctgtaagaa ttgtgcttcc
     3181 ataatattct ataatattcc gtctaatttt cacctgcaat tgaagcaact gctgccatat
     3241 ccccagtttt cttcatacct tcccttaaca taagccttcg caactttcat gcagcatatg
     3301 ctgccgtgta tgctgcaata tatgttacca catatgttgc catatatcct ttcatgcgca
     3361 tatgcaatat atcatacaaa tttctccaag tgtttcctgc ctaatataag tccctctttt
     3421 actttatcaa tgcttccctc gtatagcgga tcttcatgct cgatactaat aacaccatta
     3481 tatcctgctt c
//
LOCUS       DAOSDD010000363         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9566_length_3472_cov_6.507755, whole genome shotgun sequence.
ACCESSION   DAOSDD010000363 DAOSDD010000000
VERSION     DAOSDD010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_3472_cov_6.507755"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..723)
                     /locus_tag="PK733_19060"
                     /pseudo
     CDS             complement(205..723)
                     /locus_tag="PK733_19060"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
     gene            complement(843..1214)
                     /locus_tag="PK733_19065"
     CDS             complement(843..1214)
                     /locus_tag="PK733_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12670.1"
                     /translation="MSFDKEHDIGAYEIDEHEYPKELEKTGFHDVNVDFFTVVDYAPD
                     NAFVPDDIAIEQINGHRIGVLASTQKALNIAPDALTKHEKSTLFDLINKRSDMRVKQY
                     NDGEKLWDFTTSTILAVSGKK"
     gene            complement(1224..1589)
                     /locus_tag="PK733_19070"
     CDS             complement(1224..1589)
                     /locus_tag="PK733_19070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ12671.1"
                     /translation="MCRRGLLPQNQEIHSRYQITGIDLDTGHIEYAKAKTVQLGLDCI
                     FVNGDATAMPFADNTFDLCYSHTVAEHIPHKPFFGEQYRVLKSGGKIVVLSVRSRLGV
                     KNNHWYNTSDEEKALLEKA"
     gene            complement(1812..2228)
                     /locus_tag="PK733_19075"
     CDS             complement(1812..2228)
                     /locus_tag="PK733_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ12672.1"
                     /translation="MKYNAIFFDRDGTLTYFNPEKEKWRDLTIESWSRKPFELSYNKM
                     MILFQLAAEGKTPWYKTVDDEKEFNKRYYRHLLFGEGIADNADERALLLHNELWCNND
                     RLPFPETVEVLEYFKSRGYKMGVISDTSLSIEFCCK"
     gene            complement(2351..2452)
                     /locus_tag="PK733_19080"
     CDS             complement(2351..2452)
                     /locus_tag="PK733_19080"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ12673.1"
                     /translation="MNKGKGVTTTLERICRVLDCRIEDVVEFVPDED"
     gene            complement(2681..3118)
                     /locus_tag="PK733_19085"
     CDS             complement(2681..3118)
                     /locus_tag="PK733_19085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ12674.1"
                     /translation="MGIINAGKSNLKEWVELSQKLFPDHTFNEMFKAYDGFLTTKKEV
                     GFLYQQNNKIVGFMNVSIRNDYVNGTDTSPVVFVEAIYVMPEYRGQGIAWEFITCAEK
                     FAKEKGIKQLASDCLLENTASELFHKSCGFEEAERVICFVKNV"
     gene            complement(3118..>3472)
                     /locus_tag="PK733_19090"
     CDS             complement(3118..>3472)
                     /locus_tag="PK733_19090"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ12675.1"
                     /translation="GETNEETLIREFKEEIGADIRVNEIKWVAQIFFPWGDKPCHQIC
                     LYYIIELADETQIPLDGKFTAEEHLEGRNFDIEFHWIPLEYLKCIAVYPTNAKELLSC
                     LDDGVKHFVYREDIR"
BASE COUNT          992 a          772 c          523 g         1185 t
ORIGIN      
        1 acgcaggtta gctatctgtt ttgcagaagt cggagcgttt tcaataaaat ttatatgtaa
       61 attctccctg tatacaaaat attaacacct tcgtcaatgt cttcaagcag ttcactggca
      121 ttacttggat aaacctctat atttttaact tttgcaagag gaatctatcg gaattcaata
      181 tcaaaatctc gtccttcaat gtgctcatgg gcgataaatt ctccgtccaa cggtatctgt
      241 gtttcgtccg cctgtatatg ctaatctgtc ggtttgatat tcactcatga gccgaagctt
      301 atgggcttta tattcttttg caacatcggg ttttgcattc agataatctc tgaaatttat
      361 ataatcatgc tattctttgc tacctgttat gactacatga acatgatgag ttcttatatc
      421 ggtatttcga tcaccgcata caaaaagcat atctcccgcc acattatgtt ggcgatacat
      481 aaatcctgat cgttcaagcg caggaacaag cttcattact tcatcaaaat tgctcaccgt
      541 aacggtgata tcaattatag gcttagcctt gatattcact attgaagtac ttccgatatg
      601 ctgaatatca gttgcaaaat tgccaaatat ggcttttaac ttttctatag attgagccgc
      661 attttgttcc catgctttat catgatcaca cagttccact gttcctcgtt ttaatacaag
      721 catattttgc ctttctatac taaaatgctg atattttcta caatgtcttc cgtacttccg
      781 tttttcatta ttaatcaaaa ggtctgtagg tgtagcagaa gatttcacgg ttggtgtttt
      841 tactattttt tcccgcttac agcaagaatt gtgcttgttg taaaatccca tagtttctca
      901 ccatcattgt attgctttac ccgcatatca gaccgcttgt ttattaaatc aaacaatgtt
      961 gatttttcat gcttcgttaa agcatcaggg gcgatgttta acgctttttg cgtgcttgcc
     1021 aacactccaa ttctatggcc attgatctgc tcaattgcaa tgtcgtcggg aacaaaggca
     1081 ttgtcaggag catagtctac tactgtaaaa aagtccacat tgacatcatg aaaacctgtt
     1141 ttttcaagtt ccttcgggta ttcatgctca tctatttcgt atgctccaat gtcgtgctct
     1201 ttatcaaagc tcactgcttt acttcaagct ttttctaata aagctttttc ttcatcgctt
     1261 gtgttatacc aatgattgtt cttcactcca agtcttgagc gtacagacag aacaacaatt
     1321 tttcctcctg acttaagtac acgatattgc tcaccgaaga aaggtttgtg gggaatatgt
     1381 tcagcgaccg tatgcgaata acataaatca aaggtattgt ctgcaaatgg cattgcggta
     1441 gcatctccgt ttacaaatat acagtcaagt cctagctgta cagtttttgc ctttgcgtat
     1501 tcgatatgac ccgtatcaag atcaatcccc gtgatttggt atctggaatg tatttcttga
     1561 ttttgtggca gaagacccct ccggcacatc caacttcaag gatgttctga ccgcttttta
     1621 catcgatagc atttagccat aaagccttgt tgctatcatt aaagcgcatt cctcggctat
     1681 aatataattc ttcagtcgtc tgcacatatt gggaccatac attattcatc tttaaacctc
     1741 atttttatag cgtaactaat atttaaatat attgtattct atccttaaaa taattgcaat
     1801 gatattgtta attatttaca acaaaattca atggaaagtg aagtgtcgct gatcacaccc
     1861 attttataac ccctcgattt aaaatactca agcacttcca ctgtttcagg gaaaggcaaa
     1921 cgatcgttat tgcaccataa ttcgttatgt aacagcaacg ctcgctcgtc agcattatct
     1981 gctatacctt caccgaaaag caaatggcga taataccttt tgttaaactc cttctcatca
     2041 tcaaccgttt tgtaccaagg ggtcttgcct tcagccgcaa gttgaaaaag aatcatcatc
     2101 ttattataag ataattcaaa gggctttctg ctccaagatt ctattgtgag atctctccat
     2161 ttttcttttt cagggttaaa ataggttaat gtcccatcgc ggtcaaaaaa gatagcgttg
     2221 tatttcatat aggctcactt tccgtcagct ctgccgacaa gtaaaatatt ttataatttc
     2281 atatttccgt tttcgcttaa cacatctcgt aactctacca tattgtactt cttcaccgat
     2341 ataaaaataa tcaatcctca tccggcacaa actcaacgac atcctcaatc ctacaatcta
     2401 gtacacggca aatgcgttct agtgtggttg ttacgccttt gcctttattc atcttggcaa
     2461 ttgtagatga gctcagattg gctttgtccc caattacttt tttgacatat cccgatcaat
     2521 taacggcttc cacaatcgtt tttagctaat tgccaaaatt caatcattcc cataagattc
     2581 tttgtagaat attttaccac gataaaatac tgcaaaaaaa taaagaagtg ttttgaaaac
     2641 atccgtaaaa atgcaaacca tcatccatct ttacaccacc ttacacattc ttaacaaagc
     2701 aaataacccg ctctgcttcc tcaaaaccgc aactcttgtg gaacagttcg cttgccgtat
     2761 tctcaagcaa acaatctgac gcaagctgct ttattccttt ttccttcgca aacttttctg
     2821 cacatgtaat aaactcccat gcaatgcctt gtccccgata ttccggcata acataaattg
     2881 cttcaacaaa cacaacaggc gaagtatctg ttccgttcac ataatcattg cgaattgaca
     2941 cattcataaa acccacaatc ttgttattct gctgatataa aaatccaact tccttttttg
     3001 ttgtcaaaaa cccatcatat gccttgaaca tttcattgaa agtatgatct ggaaacaatt
     3061 tttgcgataa ttctacccat tcttttagat ttgatttgcc tgcatttata atgcccatta
     3121 ccgtatatcc tctctgtaaa caaaatgctt tacaccgtca tccaagcaac taagcagctc
     3181 ctttgcattt gtcggataaa ctgcaataca ctttaaatac tctaatggaa tccaatgaaa
     3241 ttctatatca aagttcctgc cttccaaatg ttcctctgca gtgaattttc catcaagcgg
     3301 tatctgtgtt tcatctgcaa gttcaattat ataataaaga catatttgat ggcaaggctt
     3361 atctccccat gggaagaata tttgagcaac ccacttgatt tcgtttaccc taatatctgc
     3421 gccaatttcc tctttaaatt cacggattaa agtttcttca tttgtttcgc ca
//
LOCUS       DAOSDD010000364         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9608_length_3462_cov_4.606105, whole genome shotgun sequence.
ACCESSION   DAOSDD010000364 DAOSDD010000000
VERSION     DAOSDD010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9608_length_3462_cov_4.606105"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            783..2150
                     /locus_tag="PK733_19095"
     CDS             783..2150
                     /locus_tag="PK733_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12676.1"
                     /translation="MKRILSVLICFCILLLVLLVGCKVKISIPCIFNKSASENTVSST
                     TPEDVDEIVLLKDIRAKKAKWYGNRLLYVTRNGLFKYTPSEGSILISKDIPEAKLGWF
                     DFIPSPDNTKYIMLSAELSENIVEIRDFNGDKLILKLDISKYCKSAGKIYHHVEQAGW
                     IDNETIFLSTKIRLFLINIKNGNEVQVTQECTAVLEKAQPNTEAPYLSWAYNVFKIGD
                     KLYYNSNRELFDPKPKPTSIYSGDWSAESELIKNAELMIAVDDNRFVYRSETEQGDLR
                     TFLYNIQTGQASLITDEYILDLKILRTNDGKLAFMSGNITGNKFKGVIFDPVTLEKQE
                     FDIDKYNIELDPTDHSKVFDKDKNQFGRFIGAVERNGEYVFLYIIDSYCINPERGYTN
                     KILSYSTMSKKGINIKTCEDMDEVFMSISPSGEYVAVEKYRKLTDDEFLLDVVKLNDI
                     ISYAD"
     gene            2258..3328
                     /locus_tag="PK733_19100"
     CDS             2258..3328
                     /locus_tag="PK733_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ12677.1"
                     /translation="MYDITIIGAGPAGSTLAHLLGSKYKVLLIDRRDLTKDNDSYFEK
                     CCGGLIAPDAQQMLAKLGLGVPKEILVGPQLFTVRTIDIQNDIERYYQRHYINIDREL
                     FDRWLVSMMPSSVDTRFGSLFKSYDISVNNGNIRIQFCHKGKEYNEETKYLVGADGAF
                     STVRRQLVPDLYQRSYISIQEWFQVSENQPYFSAIFDKDISDFYSWTIPKENYLIIGA
                     AILPKDNVINKFNLLKERLAKRGFKYQSSMKKNGAFILRPKNLKQIFIGKGNVALIGE
                     AAGWISPTSAEGLSYAFRSAVALAKSIEKKPGNLVGEYFKNTQDLRMNIIQKNLKLPF
                     MYDSLLRKAVMKSGLLSMNIYE"
BASE COUNT         1241 a          464 c          725 g         1032 t
ORIGIN      
        1 tcgaaatcgt ggtataggta gtattatatt agattattta gtggattatg caaaacatga
       61 tgaagtgaag tatgttggtg ttaaccagat tgaggtggaa ttaggctaat gggtgaaaag
      121 cttccggata gacccccttt gtctggtacg ttggcaattg cgggaaccca tgttgaacga
      181 ataatggtaa atgaaagtat gactaaaagg aagtaccata attgactagc accgcagagc
      241 ggtttagttc aacatggcat tgccgcttcg ggttgacttt atgtgatatg tattgcgata
      301 tttgtgccaa ctgtgcgtag tgtaaaataa ggttaccttt tcattgaaga tatggtaaaa
      361 tatatttaga aataataaaa atggtggtga tagctatgaa tacaggaaaa gaaattattt
      421 taaactaatt gatgatattc ctgagactaa agttggagaa gtgatagatc gtcttttata
      481 tttaaaaggg aaagaaagaa caagtgttat atttagctcc tagtgttata tttagctcct
      541 agtgttatat ttagctccta aggaggaaga ggaaatttgg aatatcataa atacggaaga
      601 aagagtatca tcagagaaag taaataagct tctaaaaggt gaacaaaatg agtaagattg
      661 cttatttaaa aagtgcatac aagacattag aagcatacga tggggatacc atgaggatgg
      721 tgacgttata gttgctgcag ggaagccata aaggataggg tggttgtgag gaggtattgt
      781 ttatgaaaag aatattgagt gtgctaatct gtttttgcat attgctgctt gtattgcttg
      841 ttggttgtaa agtaaaaata tctatacctt gtatctttaa caagtcggca tctgaaaata
      901 cagtatcttc tactacacca gaggatgttg atgagatagt acttctcaaa gatataagag
      961 caaaaaaagc aaaatggtat ggtaacaggc ttttatatgt tacaagaaat gggcttttta
     1021 agtacacacc ttcagaaggt tcgatcctta tttcgaaaga tattccggaa gctaaacttg
     1081 gatggtttga tttcattcct tctccggata atacaaagta catcatgctt tcagccgaat
     1141 tgtcagaaaa tattgttgaa atacgagatt ttaatggtga caaattgatt ttaaagcttg
     1201 atattagtaa atattgcaaa agcgcaggta aaatctatca tcatgtagag caagcaggtt
     1261 ggattgacaa tgagacgatc ttcctgtcta caaaaatccg gctatttctg attaacatta
     1321 aaaatggcaa cgaggtacaa gtgacccagg aatgtactgc agttcttgaa aaagcccagc
     1381 caaatacaga agcgccatat ctttcatggg cttataacgt gtttaagata ggtgataaac
     1441 tctactacaa tagtaatcgt gagctgtttg acccgaaacc aaagccaacc agtatatatt
     1501 ctggtgattg gtcggcagag agtgaattaa ttaaaaatgc tgagcttatg attgcagtcg
     1561 atgataacag gttcgtttat agaagcgaga cggagcaggg agatttaaga acatttttat
     1621 ataacatcca aacagggcaa gcttcactta taactgatga gtatattctt gatttgaaaa
     1681 ttttacgcac aaacgatggt aagcttgctt ttatgtcagg gaacatcacc ggaaataaat
     1741 ttaaaggtgt gatatttgat cctgttactt tggaaaaaca agagtttgat atagataaat
     1801 acaatattga actagatccc acagatcaca gcaaagtttt tgataaagat aaaaaccagt
     1861 tcggacgttt tataggagca gtagaaagga atggcgagta tgtgtttttg tacataattg
     1921 acagttactg cataaaccct gaaaggggat atacaaataa aatattatca tatagcacta
     1981 tgtcaaagaa gggaattaat atcaaaacct gtgaggacat ggacgaagta ttcatgagca
     2041 taagcccatc aggagagtat gttgctgtag aaaagtacag aaagctaact gatgatgaat
     2101 ttttgcttga tgtggttaaa ttaaatgata taatatctta tgctgactag ctgactaaat
     2161 gaaatttaag acaataaagg caaaattaag ctcacaaaat aaactgaaca aaagagttga
     2221 gattattttg ctcaaacatt gaaagttggg gaatataatg tatgacatca caattattgg
     2281 agctggccct gccggatcca cattagcaca tttattagga agtaagtata aggtattgct
     2341 gatagataga agggatctta cgaaagataa tgattcctac tttgaaaagt gctgcggcgg
     2401 actaatagct cctgatgcac agcaaatgct ggccaagctt ggtctgggag tacccaagga
     2461 aatactggtt ggcccgcagc tatttaccgt aagaacaatt gatattcaga atgacattga
     2521 acgttattat caaagacatt atataaacat tgaccgtgag ctatttgata ggtggctggt
     2581 ttctatgatg ccttcttcag ttgatactcg ttttggttca ttgtttaagt catatgatat
     2641 aagcgtaaac aatggaaata taaggattca gttctgccac aaaggtaagg aatataatga
     2701 agaaacgaaa tatttagtcg gagcagatgg agctttttca actgtacgaa gacagttggt
     2761 gcctgattta tatcaaagat cctatatttc tattcaggaa tggtttcaag taagtgaaaa
     2821 tcagccttat ttttcagcaa tctttgataa agatatatct gatttttact catggacaat
     2881 cccgaaagaa aactatttaa taattggagc tgcaatttta ccaaaagata atgtaattaa
     2941 taagtttaat ttactaaaag aacggctggc aaagcgtgga tttaaatatc aatcaagtat
     3001 gaaaaagaat ggggcattta tattacggcc taaaaacctt aaacagatat ttatagggaa
     3061 agggaatgtt gcattaatag gtgaggcagc cggctggata agtcctacat cagcggaagg
     3121 gttaagttat gctttccgaa gtgcagtggc tctagccaaa agtattgaaa aaaagccggg
     3181 aaatttagta ggggagtatt ttaaaaatac tcaggatcta agaatgaaca ttatacaaaa
     3241 aaatttaaag ttaccgttca tgtatgactc attacttaga aaagctgtta tgaaatcagg
     3301 attattaagt atgaatattt atgagtaaac taataaactc agtacgtaaa caataattaa
     3361 taataaaaag tcagtctaaa aatacggtgg atgtttagaa atatttgaaa tagttataaa
     3421 cataatatag ttataaacat aattttgtaa attaaaattc aa
//
LOCUS       DAOSDD010000365         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9619_length_3459_cov_5.210928, whole genome shotgun sequence.
ACCESSION   DAOSDD010000365 DAOSDD010000000
VERSION     DAOSDD010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_3459_cov_5.210928"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>372)
                     /locus_tag="PK733_19105"
     CDS             complement(<1..>372)
                     /locus_tag="PK733_19105"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ12678.1"
                     /translation="GQFLGAGKPDIAESSVKKTDMLARIFMGSAGLLFILIPGMFVRL
                     FTTDNEVIRLASTVLRIEGFSQIFLARFYVYGGLLRGTGNTKDVLITSFIGVWGIRIV
                     VCAIAVYFLNAGLVGAWIAMFL"
     assembly_gap    373..382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<383..1307)
                     /locus_tag="PK733_19110"
     CDS             complement(<383..1307)
                     /locus_tag="PK733_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ12679.1"
                     /translation="MNQITKTENLNRTVIFIVYPIFLELFLQTIIGYIDVIMIGRIGT
                     NELAAIQICNSPIDTIITIFTAFGIGCTALVTRYVGAEDNENVKRCIGQTILISFVVS
                     TITFIIFNLFRRQVLTLMGADENIIDSADTYIKFISYSIPCLIFSTLMYGLIRGIGNT
                     KIPMYINIIQNIIHLILNFFFIYGTRSIHISSKWKINIPGLDMGIKGAAISTLISRIW
                     CVIGILTYYGIIQKTIIPKKYLKPDKGMLKSIMNIGFPASFEQMIFKGGMLTFTRIVV
                     SLGNVSLAAHAVAETAEMVSYLPGLAFQIATI"
     gene            complement(1380..2336)
                     /locus_tag="PK733_19115"
     CDS             complement(1380..2336)
                     /locus_tag="PK733_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ12680.1"
                     /translation="MSKYVKISSLGRTLISANEIEDGSYGLVENVKALWQKELDKVLP
                     DKPDLIVLPECCDIVYGLTDEKRIEYNKVKGYEIRNFFMNVAKENNCYIAYSASRLLP
                     DGTKRNSTQMIDRNGNIAGIYNKNHLTINDSENNGTLYGADANVIQCDFGKVACAICF
                     DLNFDELRLKYIPQKPDLIVFSSLYHGGLMQNYWAYSCRSYFVGCVSDDENTIISPVG
                     EIIAKSTNYYDYVTHTINLDYIICHLDYNRPKLQNLKMKYGRKVKIFDPGHLGSVLIT
                     SETEEYTAMDFAKEFELELLDEYFERCREHRSIPGKVEKTEK"
     gene            complement(2452..>3459)
                     /locus_tag="PK733_19120"
     CDS             complement(2452..>3459)
                     /locus_tag="PK733_19120"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12681.1"
                     /translation="TSDYREILIDSEVDGVMILTTHNIREKIIKDAAEAEKHIYVEKP
                     MSTSVEESYRIMKIVRQTGVKLVVGFNRRCAPAIEDAIKIYEDHKANPVDAPWRFKRN
                     EGNHQFLREEEATMVMMRINDDLMSFKPYALDEMIGEGSIIGELCHFIDLACYITKKE
                     PIKVYAEGWARVNQILTLQFEDQSIASIFETGVGSFDHTKELIEIYHKGASIAMDHFL
                     QLRVGGIEGIYKKNYPFWNDPYPYITDGEGTNLFINKVKERNRIATDKGSAGYPSVDK
                     GHYRMLDRFADCILLDAPSPCNELDGSRASLVTFKARESVRLGIPVKISRDEYDFYFA
                     L"
BASE COUNT         1061 a          692 c          480 g         1216 t
ORIGIN      
        1 taaaaacatt gcaatccaag cacctactaa acccgcattg aggaaataca ccgctatggc
       61 gcacacaaca atcctaatgc cccaaacacc gataaatgac gttattaaga catctttagt
      121 gttgcctgtc cctcttaaga ggccaccata gacataaaat cttgcaagga atatttgtga
      181 aaacccttcg attctgagaa cagttgaggc aagccttatt acttcattgt ctgtagtaaa
      241 taatctaaca aacattccgg gtatcaatat gaagagcaat ccggcactcc ccataaaaat
      301 ccttgccagc atatctgttt tctttacgct cgactctgca atgtcaggct tccctgctcc
      361 aagaaattgt ccnnnnnnnn nnttattgtt gcaatctgaa atgctaaacc cggcaaatat
      421 gataccattt ctgccgtttc tgccactgca tgggctgcta gtgatacatt tcccaaactc
      481 acaacgattc ttgtaaacgt aagcattcca cctttgaata tcatttgctc gaaggaggcc
      541 ggaaacccta tattcataat agacttcagc atacccttat caggttttaa atattttttg
      601 ggtataattg ttttctgtat aataccataa taagtgagta tacctattac acaccaaatc
      661 ctgcttataa gagttgaaat tgcagcacct tttattccca tatcaaggcc aggtatattt
      721 attttccatt tgctggaaat atgtatagat ctagtgccat atataaagaa aaagtttagt
      781 atcaaatgta taatgttttg aattatattt atatacatag gaatttttgt atttcctatt
      841 cctctaatta aaccatacat tagcgtacta aaaataagac atggaatact gtatgaaata
      901 aattttatat atgtatcagc actatcaatt atgttttcat cggctcccat cagagttaaa
      961 acctgacgcc tgaataaatt aaatattata aatgttatgg ttgaaacaac aaaagatatg
     1021 agaatggttt gtcctataca ccttttaaca ttctcgttat cttcggctcc aacatatctt
     1081 gtaactaaag ctgtacaccc tataccaaaa gcggtaaata tagtaattat ggtatctatg
     1141 ggcgaattgc aaatttgtat ggcagcaagc tcatttgtac caatccgccc tatcattatt
     1201 acatcaatgt agcctattat agtttgaaga aacaattcaa gaaatatagg atacactata
     1261 aatataaccg ttctgttcag gttttctgtt tttgttattt ggttcattta actgtatgcc
     1321 tttcaaaata agtataataa gcataaaata aatagtctgc ttcccaattg ttcttcagtc
     1381 tatttttcag tcttttcaac ttttccgggt atgcttctgt gctccctgca tctttcaaag
     1441 tattcatcaa gcaattcaag ttcaaattct tttgcaaaat ccattgctgt gtactcttcc
     1501 gtttcactag tgattaatac tgaacctaaa tgtccggggt caaatatttt aacctttcta
     1561 ccatacttca tcttaagatt ttgtagttta ggtcggttgt aatctaaatg gcaaataata
     1621 taatctaaat ttatagtatg agttacgtaa tcataatagt tggtgctttt tgcaattatt
     1681 tctcctacag gtgagataat tgtattctca tcatcagaca cacatcctac aaaatatgat
     1741 cggcaggaat atgcccaata attttgcatt aatcctccat gatataagga tgaaaacact
     1801 ataagatcag gtttctgagg tatgtatttg agccttaact catcaaaatt aagatcaaaa
     1861 caaatagcac aagccacttt gccaaaatca cattgtatta catttgcatc cgctccatat
     1921 agggttccat tattttcgct atcatttatg gttaaatgat ttttattata tatccctgca
     1981 atattaccat ttctgtcaat catttgagta gaatttcttt ttgttccatc aggtaaaagt
     2041 cttgaggcag agtatgctat atagcaattg ttttcttttg ctacattcat gaaaaaattc
     2101 cttatttcat atccttttac cttattatat tctattctct tctcatcggt aagaccataa
     2161 actatatcac aacactcagg taatactatt aaatccggct tatctggtaa taccttatca
     2221 agttcttttt gccataaggc tttcacgttt tctactaatc cataagaacc atcttctatc
     2281 tcattagcac taattagggt cctacccaaa gaacttattt ttacatattt tgacatatac
     2341 gagctccttc ccttttccat aaaaatccat tcataacata aataattatc aggttttcat
     2401 gttctctatg gcaacttatg ggatatttat taattattac acgccacaaa attacaatgc
     2461 aaaataaaaa tcgtactcat ccctgcttat ttttacagga atgccaagcc gtacagactc
     2521 tctggcctta aaagtgacta atgatgctct tgaaccgtca agttcattgc atggtgaagg
     2581 tgcatcaagt aaaatgcagt ctgcaaaacg gtcaagcatc ctgtaatgac ctttatctac
     2641 tgaaggatat ccggcacttc ctttatcagt agcaatacga tttctttcct ttactttgtt
     2701 tatgaataaa ttagtccctt ctccatctgt aatatacggg tatggatcat tccagaaagg
     2761 gtaattcttt ttatatatac cttctatacc tccaactctt aattgtagaa aatgatccat
     2821 tgctatagat gcccctttat ggtatatttc tattagttct ttggtgtgat caaagcttcc
     2881 tacacctgtt tcaaaaatag atgcaataga ttgatcttca aactgaagag ttaaaatttg
     2941 attaacccgc gcccatcctt cagcataaac tttgattggt tccttttttg ttatataaca
     3001 agctaaatct ataaaatggc ataattctcc tattattgag ccttcgccaa tcatttcatc
     3061 aagggcataa ggtttaaatg acattaaatc atcattaatt ctcatcataa ccatagttgc
     3121 ttcttcttca cgtagaaact gatggttacc ttcatttctt ttaaatctcc atggagcatc
     3181 aaccggattt gctttgtgat cttcatatat tttaattgca tcttctattg cgggcgcaca
     3241 tctcctgtta aaaccgacaa caagcttaac tcctgtctgc cttacaattt tcataattct
     3301 ataggattct tctactgatg tactcatagg cttctctaca taaatatgct tctctgcctc
     3361 tgctgcatcc tttattattt tttctcttat attatgagtt gtgaggatca ttactccatc
     3421 tacttcagag tctattaata tttctctgta atcagaagt
//
LOCUS       DAOSDD010000366         3447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9661_length_3447_cov_4.685436, whole genome shotgun sequence.
ACCESSION   DAOSDD010000366 DAOSDD010000000
VERSION     DAOSDD010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9661_length_3447_cov_4.685436"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..681)
                     /locus_tag="PK733_19125"
     CDS             complement(352..681)
                     /locus_tag="PK733_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOJ12682.1"
                     /translation="MKVEYRDGLLFTTIELVQDGKRKIIENIVIDIGASNSVISQNCV
                     DDIDIRFTGKEEIVTTFGIGGKEHAFVKKIDKIILGDLVLEGYEADFAGYMYKRKCQI
                     YEEPIIM"
     gene            complement(1632..1757)
                     /locus_tag="PK733_19130"
     CDS             complement(1632..1757)
                     /locus_tag="PK733_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12683.1"
                     /translation="MLSERGKRELNHLSAGEGHGRREESGSTYLRTLRRMGSGML"
     gene            complement(1727..2524)
                     /locus_tag="PK733_19135"
     CDS             complement(1727..2524)
                     /locus_tag="PK733_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ12684.1"
                     /translation="MFLGKLYFTWRRYFRWIFNLSVRYASTFKEVELPYIVSSHKTPL
                     YRQLRNVDMWLQENKVTNLKLACGKLNRIILKSGETFSFWRLVGKPTKAKGFLKGMVL
                     TNGSFSAGVGGGLCQLSNLIYWMTLHTPLTVTERWRHTHDVFPDANRIQPFGSGATIV
                     YNYIDLQIKNNTPHEYQLNIRVGENDLEGEWRCEQPCLCKYKVYESEHLIRQEWWGDY
                     MRHNVIRRIVLDDNDNKIADEFITENHAIMMYEPMLGECYPKGAKES"
     gene            complement(2959..3372)
                     /locus_tag="PK733_19140"
     CDS             complement(2959..3372)
                     /locus_tag="PK733_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOJ12685.1"
                     /translation="MDNLYQAQEWQRLAGMDLHSAEFLLNMHPVPLEIICYHCQQSAE
                     KYLKGYLVLNGKNPAKTHDLDELCKLCADMSDTFTEIADHCSDLTAYGVQPRYPMEMM
                     LEERDILQALNSAKVIRDFILNLVPKIMQDKQEQS"
BASE COUNT         1013 a          716 c          486 g         1232 t
ORIGIN      
        1 ttatgagtac ctggaaaatt ttcagacttc agcataatag cttataaaat tatttgctct
       61 atacagtaac ccatatttag ccttgccttg ttatgctttt ttgccgatat aatatcgttt
      121 ataatatcat ttttcatctg tttataaagt atcttcttcc attttctcat atatctgttt
      181 tcaattttca aggatcatca ctgctctttt cttgtccaat cattttatta ccgtttatca
      241 tcagttttca ttacttattt gacactacca aaaatagtac cggtaatata tatatttccc
      301 tgccggtact atatgtatga atatatgctt gagtttgatg catttgatat attacattat
      361 aatcggttct tcatagattt ggcattttcg tttatacata taccctgcaa agtccgcctc
      421 atatccctct agtactaaat cccctaatat tatcttatca attttcttga caaaggcatg
      481 ttctttccca ccgatgccaa aagtcgttac tatttcttct tttccggtaa acctaatatc
      541 aatatcatct acacaatttt gtgaaattac tgaattagaa gctcctatat ctatcacgat
      601 attctcaatt atcttcctct taccatcttg tacaagttct atggtagtaa aaagcaatcc
      661 atccctatat tcgactttca tttcttatac tctccttaat cccattctat ttcttaacct
      721 tataataact tcttcttttg aagtatgata taccaactcg tcacctttgg aagataaaag
      781 ttactttgtt gcttttgatt catctacagt tttgataaca gccacatcac atactttcta
      841 cttatcttct tctatgcaag attctaaaat tttgattttg acaaactatt cggaaatagc
      901 tctctaactt cttcccattt cattcatttc actccccttt taatattatt atatattata
      961 gcactaaaaa gcattacatt cagtgttttc ttttggatat aatgtgaaga aaaagcttca
     1021 cattttgagt ttataattat aaattgccag cttaatttaa acagtaaaaa cagttaataa
     1081 acaagcaatt aaagacaaag ctgcctcacc ataatttttt agctttatat aaataataat
     1141 agataaaaat aatacaacta tcacagtaac acccatcatt gcttgtgtgt tattaattac
     1201 taaatatatt actcaaaata gtaaactact aaaaaccaca ataaatgtcg gtacaagttt
     1261 atctatagct tttattgtgt tgcctaggga tttagcggtc ccaaaaaaca tctttgaact
     1321 tttccatttt ttcttgatac ttatttatcc catctttcta attaaatctg aagtctaaca
     1381 gttggcactc gaggcatgtt tatgctcaca ggtcagctga aaatctagat aacttttatc
     1441 ttagtttcta gatgagcgac ttagttagca aaagtgtttg gtatattatt agaacttttc
     1501 gatttcagaa ttatgacctt atccgattcc tcacacgact tcttcctgaa tttcacgctt
     1561 ggtggcttat atcaggaatt acaatactgc ctattgatcg tatgaggtct cccatgttcc
     1621 attttcagct ttcatagcat tccgcttccc atacgccgaa gagttcttaa gtaggtgctt
     1681 ccggactctt cccgccttcc atggccttcg ccagcactca agtggttcag ctctcttttg
     1741 cccctttcgg ataacattcc cccaacatcg gctcatacat cataatagca tgattttccg
     1801 taataaattc atctgcaatt ttgttatcgt tatcatccaa aacaattcgc cttataacat
     1861 tatggcgcat gtaatcaccc caccattctt gtcttatcaa atgctcgctc tcataaactt
     1921 tgtatttaca taaacaaggt tgttcacatc tccattcgcc ttctaaatca ttttctccta
     1981 ccctgatatt aagttgatat tcatgtggag tattattttt aatttgtaaa tctatgtaat
     2041 tataaacaat cgttgcacca cttccaaatg gctgaattcg atttgcatca gggaaaacat
     2101 cgtgtgtatg ccgccaacgt tccgtaactg ttaatggcgt gtgaagagtc atccaataaa
     2161 tgagatttga aagctgacat aaaccgccgc ctactcctgc agaaaatgac ccatttgtca
     2221 aaaccattcc tttcaaaaag ccctttgcct tcgtcggttt tccaaccaaa cgccaaaatg
     2281 aaaaggtttc tcctgatttt agtattatcc tattaagctt tccacaagct aactttaagt
     2341 tagttacttt attctcttgc agccacatat caacatttct caattgcctg tataatggcg
     2401 ttttatgaga cgacactata taaggaagtt caacctcctt aaaagtagag gcatatctaa
     2461 ccgatagatt aaatatccac ctaaaatatc ttctccaagt gaagtacaat ttcccgagga
     2521 acattcgaat atttgaacgg agaataggcc tactagtttt aaaatgatag tctagagcca
     2581 gaattatccc ctcccttccc tacctttgca gatatgtcat ataacgacta gcactcaaga
     2641 tgtgcctaag cttacaagtc agacggaaac cttagatagc tcttatcttg gtttctggct
     2701 gaccgactca gttagcgaag gcatgtgata tgctatatag aactctggtt tcaggaacca
     2761 ttattcctgg gctcaggtct tgcacatctt aatttgagtg cattgttagg cagagtatcg
     2821 ataaatccag tcgcacagtg ctcgggataa aatatgggtt tacaatgtta gtgtgaacaa
     2881 catcttttgg gcaatagctc atttcacaat aatattttac aaaggaaaac aaatattaaa
     2941 attatatatc ggtagttttt aagactgttc ttgtttatcc tgcattattt tcggtacaag
     3001 atttagaata aaatctctaa ttacttttgc gctatttaaa gcctgcaaaa tatcccgttc
     3061 ttctagcatc atttccattg gatacctcgg ctgaacaccg taagctgtta agtctgagca
     3121 atgatcagct atttctgtaa atgtatcaga catgtctgca cagagtttgc acaattcgtc
     3181 caaatcatgt gtcttagcag gattctttcc gtttaaaacc agatatcctt taagatactt
     3241 ttcggcagat tgttgacaat ggtaacatat aatttccaaa ggaacagggt gcatatttag
     3301 gaggaactct gcactgtgca gatccattcc agcaagtctt tgccattctt gagcttgata
     3361 taggttatcc atacagcacc accccctctt ttgctatttg ccgttcaata gtggggttgg
     3421 attttcgttt attaaactta ttttctt
//
LOCUS       DAOSDD010000367         3427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9738_length_3427_cov_5.855279, whole genome shotgun sequence.
ACCESSION   DAOSDD010000367 DAOSDD010000000
VERSION     DAOSDD010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9738_length_3427_cov_5.855279"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK733_19145"
     CDS             complement(<1..608)
                     /locus_tag="PK733_19145"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOJ12686.1"
                     /translation="MLNEKIWVHNEIDEKDIDKLANETGVSRLIAKVLFGRGMKDEEY
                     IKAFLNPVLKFFHDPFLMKDMEKAVERIIRAIDKKEKITLYGDYDVDGVTGIAILYDF
                     LQSLGADATFYIPDRLEEGYGVSKDALEVLIKQGSSLILTIDCGITAVYEAAYAKANN
                     VDMIITDHHECGETLPDACAVVNPCQPDCKYPFSKLAGAGVV"
     gene            1005..2600
                     /locus_tag="PK733_19150"
     CDS             1005..2600
                     /locus_tag="PK733_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOJ12687.1"
                     /translation="MESLKLKIKDEVSKRKTFAIISHPDAGKTTLTEKLLLYGGAIRL
                     AGTVKSRKASKYASSDWMEIEKQRGISVTSSVLQFKYNGYCVNLLDTPGHQDFSEDTY
                     RTLMAADSAVMLIDGARGVEEQTIKLFHVCKMRGIPIFTFVNKMDRASKDPLELMEEI
                     ENVLDIRSCPMNWPIGIHGDFKGVYNRQLGQVELFTGGNHGRTAVVSTIENMDDYTLG
                     QQLGSYYYDRLKDEINLLNEAGDGFDKEKIMKGELSPMFFGSAMTNFGVQPFLEEFLQ
                     LAPSPGMRNLSAGGVLPDSEKFTGFVFKIQANMNPAHRDRIAFIRICSGRFTRGMPVY
                     HVQGEKEVKLAQPQQFMAQDRIMVEEAYAGDIIGIYDPGIFSIGDTLSEDADVKFERI
                     PIFPAEHFARVTAKDSMKRKQFIKGISQLSEEGAIQVFKEPDIGVEELVIGAVGELQF
                     DVLEYRLKYEYGVDIRMQRLPLRYARWIDGSIDNLAKLNLTSSTLIVEDKLERLVLLF
                     ENEWSIRWAQERNKILKLKDIAA"
     gene            complement(2939..>3427)
                     /locus_tag="PK733_19155"
     CDS             complement(2939..>3427)
                     /locus_tag="PK733_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12688.1"
                     /translation="LKAPIIRVWAGNRGSDKADRAWWDLVINESRRIADMALKEGVTV
                     AYEYHNNTLTDTSESAYRLLTEVNHKNMLSYWQPPLNIGEEERIEGLKKITPWLSNIH
                     TYYWESGNKMVLEAGIEKWLKYMEIIKGVEGTRYCMIEFVKGDAPEQFIEDAEALKKI
                     VL"
BASE COUNT         1087 a          622 c          643 g         1075 t
ORIGIN      
        1 aatacaacac ctgctcctgc aagtttacta aagggatatt tgcaatccgg ctggcatgga
       61 tttactactg cacaggcatc aggcagtgtt tcgccacatt catgatgatc tgttattatc
      121 atatctacat tgtttgcttt tgcataagct gcctcataga cagcagtaat accacaatct
      181 atagttaata ttaaactgga accctgcttt attaaaacct ccaaagcatc tttcgaaact
      241 ccatatccct cttcaagtct gtcaggtata tagaaggttg catcagctcc caagctttgt
      301 aaaaagtcgt ataggattgc aatacctgta accccatcca catcataatc accgtatagg
      361 gttattttct cttttttatc tatcgccctt attatccttt caacagcctt ttccatatcc
      421 ttcatcagaa aaggatcatg aaagaactta agaacaggat taagaaaggc tttaatatat
      481 tcctcatctt tcataccccg gccaaacagt actttggcaa ttaatcttga aactccggtt
      541 tcatttgcca gcttgtcaat atctttttca tcaatttcat tatggaccca aattttctca
      601 tttaacatgg catcacgtca catcacatca catcacccgt aaaatatttt gttaaacgac
      661 atagcttgtc ttttaaccat tgattcaatt atagattatt tgcttgtata gttcaaacaa
      721 ataattatct atatagggtg catacttaaa aaaccagtaa tgccacattt tgcagtgtat
      781 acagaaaata cacccgtatc ctaatatttt aattgacaga agttaagaat ataatataaa
      841 atgatttctg ttggaattag ctataaacag ctaaaaacag ttaaaagagc tgaaagaagc
      901 aaggcagact aaaaaaataa ctaaaggaaa actgatactc tttttagagt aaattgatgt
      961 gtttataata gcgacatgta tagaataaag agagaggata attaatggag agtttgaaat
     1021 tgaaaattaa agatgaggta tcaaagagaa aaacctttgc aattatatct catcctgacg
     1081 caggtaaaac tacgcttact gaaaagctac tattatatgg tggtgcaata aggcttgcag
     1141 gtacagtaaa atccaggaaa gcaagtaaat atgcttcatc agattggatg gaaattgaaa
     1201 aacaaagagg tatttctgta acctccagtg tattgcaatt taaatataac ggatactgtg
     1261 ttaatttact ggatactccc gggcaccagg attttagtga agacacgtac agaacactta
     1321 tggcggcaga tagtgcagtt atgttaatag atggtgcaag gggtgttgag gagcaaacca
     1381 taaagctttt tcatgtttgt aaaatgagag gtatacccat ttttacattt gtaaacaaaa
     1441 tggatagggc aagcaaagat cccctggaat taatggaaga aattgagaac gtccttgata
     1501 tacgttcctg cccaatgaac tggcccattg gaattcatgg tgattttaaa ggggtatata
     1561 acaggcagct tggacaagta gaacttttta cagggggaaa tcatggcagg actgctgttg
     1621 tttctaccat agaaaatatg gatgattaca ctcttggtca gcagcttggc agttattact
     1681 atgataggct gaaggatgaa ataaacttgc ttaacgaagc cggtgacggg tttgataagg
     1741 aaaaaataat gaagggtgag ctttcaccta tgttttttgg tagtgcaatg acaaatttcg
     1801 gtgtacaacc cttccttgaa gaatttctac aactggctcc ttccccgggt atgagaaatt
     1861 tatctgccgg tggtgtactg cccgatagcg aaaaatttac tgggtttgtt tttaaaatac
     1921 aagctaatat gaacccggct catagagata gaatagcttt tataaggata tgttcaggac
     1981 gctttacccg cggtatgccg gtttatcacg tgcaaggtga aaaagaggtt aaactggctc
     2041 agcctcaaca atttatggca caagatcgta ttatggtaga agaagcttat gccggtgata
     2101 ttattggtat atatgatcca ggtattttca gtataggaga taccctttca gaagatgctg
     2161 atgtcaaatt tgagagaata cctattttcc ctgctgaaca ttttgccaga gtcacagcaa
     2221 aggattcaat gaaacgcaag cagtttataa aagggatatc acagctttca gaagagggtg
     2281 ctattcaagt ctttaaagaa ccggatatag gcgttgaaga acttgttatt ggagctgtag
     2341 gagaattaca attcgatgta ttggaatata ggctcaaata tgaatatggt gtagacatca
     2401 gaatgcagcg tcttccctta aggtacgccc gctggattga cggaagtata gataacctgg
     2461 caaaattaaa cctgacaagt tctactctga tcgtggaaga taaattagag cgtcttgtgt
     2521 tattatttga aaacgagtgg tctataaggt gggcacagga gagaaacaag attttaaaat
     2581 tgaaagatat tgctgcttaa aattcagata acccgcaact cttaaatgtt gcgggttatc
     2641 ttttcattta taatatttga tatataatat gattgttccc cagaaagccg ttttacggct
     2701 tttcactgca ggattatctg cagtgagttg caatttcgtt gcaacctggt gcaatctaat
     2761 atggttatcc agccttcttt aagaaggctg atgaaatccg atgtgcctta aatttcatct
     2821 gaaagtcatt tcatgacttt cagggtgata accataatat acaatattta actttacatt
     2881 atagatattg tatctgataa cgaaatttct tggagctgta cttagattat gatgcaattt
     2941 acagtacaat ttttttcaaa gcttctgcat cttcaataaa ctgttcaggc gcatcgccct
     3001 taacaaactc aatcatacag taccttgtac cttctacacc tttaattatt tccatatact
     3061 tcaaccattt ttcgattcca gcttcaagaa ccatcttatt accggattcc caatagtaag
     3121 tgtgtatgtt actcagccat ggagttattt ttttcagccc ctctatacgc tcttcttctc
     3181 caatattcag aggtggttgc caataggaaa gcatgttctt atggttaacc tctgttaaaa
     3241 gtctgtaagc tgattctgaa gtatcggtaa gagtattgtt atgatattca tatgccacag
     3301 taactccttc ttttagtgcc atatcagcta ttcttcttga ttcatttatt accaggtccc
     3361 accatgcccg gtcagcctta tctgaacctc tattccctgc ccataccctt ataatgggag
     3421 cttttag
//
LOCUS       DAOSDD010000368         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9776_length_3416_cov_4.439453, whole genome shotgun sequence.
ACCESSION   DAOSDD010000368 DAOSDD010000000
VERSION     DAOSDD010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9776_length_3416_cov_4.439453"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PK733_19160"
     CDS             complement(<1..1528)
                     /locus_tag="PK733_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ12689.1"
                     /translation="MMEIYNAGLYCRLSREDENSNGNRQSESIKNQADFLSLYVIEKG
                     WNIFDIYADDGYSGTSFDRPDFKRLICDIEAGKVNLVLVKDLSRLGRDYIETGLYLEK
                     FFPEKNIRFIAVNDGVDTFSENSNNDMSPFKSVINDMYAKDISKKVITVFRSKAAAGE
                     FIGAFAPYGYKKDTADRNKLIIDNVVASVVKRIFKMYIEGIGLSQIAHTLNNEKIPNP
                     ATYKATNSNYVNSRIKNELWSHSTIKAILTNMTYTGNLAQGKYRKVSYKIKKLRVVDR
                     RDWIVVENTHEPVISQEDFDLVQAMMYRKSSGLVLSKKTNKPLSGFVFCGDCGEYMTF
                     TKTKKDIEYLICSKYKRYTSKYCTRHSVKVVDIEAAILVDINSIIKAVINIRSISEDI
                     SIDSFCNKGNEIEKEIRQLKKEIADIGSVIKSLYRDKVKGTISEEDFINLNKEFSKER
                     DILVYRCKELNNIKQQEDKNRDGVNDLLKTIKNLTQPKKLTSELLERLIEKIEVFENG
                     S"
     gene            complement(1561..1683)
                     /locus_tag="PK733_19165"
     CDS             complement(1561..1683)
                     /locus_tag="PK733_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12690.1"
                     /translation="MSKNLIEVCHKIEVSHEFTGEKTLEEIVKEYVQQIINRKC"
     gene            complement(1941..3305)
                     /locus_tag="PK733_19170"
     CDS             complement(1941..3305)
                     /locus_tag="PK733_19170"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HOJ12691.1"
                     /translation="MIKNTKDILSYGVEKAECILREFSLKVEGMEAQGSLTIKSMENL
                     LGIMFAQFKDIGLIMAGEYFSNLELEEIDEYCDCGKKLVKVKESSLTRIQSIYGYMPI
                     KRDTVFCRRCHKGYGIIDKEIGIYGEHRITKGMTERLAYIGQLLPFERGTEVLNKLTG
                     VEVSASLIETVSEEIGKEVFNKEIHEAKEAWDKPEESIPQELPRYRKEARLYIMTDGL
                     QVNTRVEDENGSTWKEMKLGLVFCDKDVIKKGKDSHIITKKEYVSYFGGINEFKKVLF
                     AAAARAGYGKIAELVVIGDGAAWIWNMCEELFPDAVQILDFYHLSENTYNYSKLLYTE
                     DEAGRKKWVKDVLDAITTGRIDEAIKYIEVKKPEKIPDGVVNLYTYFTNNRGRIDYKY
                     YKEKGYYIGSGAIESGNKMVIQQRMKQSGMRWSINGGQYIAALRTKHESNKWNDVTKV
                     IYAA"
BASE COUNT          935 a          745 c          412 g         1324 t
ORIGIN      
        1 tacttccatt ctcaaaaact tcaatcttct cgataagcct ctccagcaat tcactagtca
       61 gctttttggg ctgtgtaagg ttcttgattg tttttaataa atcattgacc ccatctctat
      121 tcttatcttc ttgttgtttt atattgttga gctctttaca tctgtataca agaatatctc
      181 tttctttaga aaattccttg ttcaaattta tgaaatcttc ttcgctaatc gttcctttaa
      241 ctttatccct ataaaggctt ttaatgacgc ttcctatatc tgctatttct tttttaagct
      301 gtcttatttc cttttcaatt tcgtttccct tattgcaaaa gctatcaata ctaatgtctt
      361 cggaaatact tcttatattg attactgcct ttattatgga attaatatct actaaaatag
      421 cagcttctat gtcaacaact tttactgaat gtctggtaca gtatttagag gtataccttt
      481 tatattttga acatattaaa tattctatat cttttttagt tttagtaaaa gtcatgtatt
      541 ctccacaatc accacaaaac acaaatcctg ataacggctt atttgttttt ttacttaata
      601 ctaacccact gctttttcta tacatcattg cttgaacaag atcgaagtcc tcctgtgaaa
      661 tgactggttc atgagtattt tcaactacaa tccaatctct tctatcaaca actcgaagtt
      721 tttttatctt gtaacttact tttcgatact tgccctgtgc taaattacct gtatatgtca
      781 tatttgttag aatagctttg atagtgctat gtgaccatag ttcatttttt atcctgctat
      841 taacataatt actgttagta gctttataag ttgccgggtt aggaatcttt tcattattta
      901 aggtatgtgc aatttgtgat aaaccgatac cttcaatata cattttaaat attcttttga
      961 ccactgaagc aactacatta tcaatgataa gcttatttct atcagcagta tcctttttat
     1021 atccgtatgg ggcaaatgca cctatgaact ctcctgctgc tgcttttgac ctaaacacgg
     1081 ttataacctt tttacttata tcttttgcat acatatcgtt gataactgac ttgaaaggac
     1141 tcatatcatt attgctgttt tcgctaaaag tatcaacccc atcatttaca gctatgaatc
     1201 ttatattttt ttccgggaag aatttttcca aatatagtcc ggtttcaata tagtctcttc
     1261 caagccttga taaatctttt actaatacaa gattaacctt accagcttca atatcacaga
     1321 ttaacctttt aaaatcaggt ctgtcaaagc tcgtgcctga atacccgtca tcagcataaa
     1381 tgtcaaaaat attccagcct ttttcaatta catataaaga caaaaaatct gcttgattct
     1441 taatactttc tgattgccta ttaccgttac tattttcgtc ctctcttgag agtctacagt
     1501 atagtcctgc attatatatt tccatcattg tgttaactcc tcccattttc ctttaagaag
     1561 ttaacacttc ctgttaataa tttgctgaac gtactcttta actatttcct cgagtgtctt
     1621 ttcgccggta aactcgtgag acacttctat tttgtgacat acttctatca aatttttaga
     1681 cataatgatc ccttcgcaca aacattctag taatttatat gaaccggact gcttgtataa
     1741 tgctttatgc gaagaaataa ttattgaaca cctcgtattc tattgggtgt acattttatt
     1801 tctgtcacac attttgtaaa gtttctgtct gatttacaat aaaggcaaga acttcacata
     1861 tgtgaatatc ctaccagtaa aaaagggcat aaatatccaa tttacgcatc cggtattttc
     1921 caagtatctt taattatata ttaagctgca tatatcactt tggtaacatc attccattta
     1981 ttactttcgt gcttggtacg aagggctgct atatactgtc ctccattaat actccatctc
     2041 atgcccgatt gcttcatgcg ttgctgtata accattttat taccgctttc tatagccccg
     2101 cttcctatat aataaccctt ctctttgtaa tacttataat ctatacgtcc tcggttgttt
     2161 gtaaaatagg tatataagtt cactactcca tctggaattt tctctggttt tttcacttct
     2221 atatacttta tggcctcatc aatcctacct gtagtaatgg catcaagcac atctttaacc
     2281 cacttctttc ggcctgcctc atcttcagta tataacaatt ttgaataatt gtatgtgttc
     2341 tcgcttagat gataaaaatc tagtatttgc accgcatcag gaaataactc ctcacacatg
     2401 ttccatatcc atgctgcacc atcccctatt acgactaact cagctatctt tccataccct
     2461 gcccttgctg ctgctgcaaa tagcactttt ttaaattcgt ttattccccc gaaatacgat
     2521 acgtattcct tctttgttat aatatgacta tccttccctt tctttatgac atccttatca
     2581 caaaatacga gccccagttt catttctttc catgtagaac cattctcatc ttctacacga
     2641 gtattcacct gaagaccatc tgtcataata taaagtcttg cttctttccg ataacgcgga
     2701 agctcttgcg gaatagattc ctccggctta tcccatgcct cttttgcttc atgtatttct
     2761 ttattaaata cttccttacc aatttcctca gacaccgttt caataagact tgcacttact
     2821 tccacaccag ttagtttatt taaaacttct gtcccccgtt caaaaggaag taattgccct
     2881 atatatgcca acctttcggt catccctttg gttattctat gttccccata tattcctatc
     2941 tccttatcaa taattccata tcctttatgg catctgcggc aaaaaactgt atctctttta
     3001 ataggcatat acccatagat cgattgtata cgtgttaaac tgctttcttt aacttttacg
     3061 agctttttac cacaatcgca atattcatct atttcctcaa gttccagatt actaaaatat
     3121 tcacctgcca ttataagccc tatatcctta aattgagcaa acataattcc taatagattt
     3181 tccatgcttt tgatagtaag gctcccttgt gcttccattc cttctacttt tagtgaaaat
     3241 tcccttaata tacattcggc tttttcaaca ccatatgata aaatatcctt agtgtttttt
     3301 atcattgtct aagaccccct tttttttctt aagggtatct tagacttttt tctttccttt
     3361 tttcaatgga aatgtatgcc ttggataatt attcttcact taaataatgt acaccc
//
LOCUS       DAOSDD010000369         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9834_length_3401_cov_5.378063, whole genome shotgun sequence.
ACCESSION   DAOSDD010000369 DAOSDD010000000
VERSION     DAOSDD010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9834_length_3401_cov_5.378063"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK733_19175"
     CDS             complement(<1..231)
                     /locus_tag="PK733_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ12692.1"
                     /translation="MTGKEVILTYEGLEKLEKELEFLKITKRREIAERIKQALSFGDI
                     SENSEYDEAKNEQAYVETKIVQIETMLKNAKVI"
     gene            complement(502..1596)
                     /locus_tag="PK733_19180"
     CDS             complement(502..1596)
                     /locus_tag="PK733_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12693.1"
                     /translation="MNNILLVAAGQRNEGRAGVLQSRISYLLDKKFYKKRITEYINKS
                     LMDIESSSNGNIFLTELPYYVLEPDFYSWKGKDRAADIKNSILEKIGSLCFEKETDRC
                     YFSNKLFAIPGFEIQVLQETYKFQTAQVKQTSHNRSKKEIMYPGKVLFRALTLDILKK
                     ICNDLKIDIRQSDVVIIGDVEDKEEVLKTIRKLSLVIKYLTVLLYDEDVIWLKNELED
                     IYAATGLAVNLACNLGGGAYNALRNADIILNYGCVESCVQNLVTCSKLKAETVIVNNS
                     FTHLNISFKKPGLKNPLIEGIVTNISKAIPDKFRELIPEQFTSAVAAEILTGFRTGWV
                     QEEMAKRFKSDGYYIKGFTGFNTIDIKPAS"
     gene            complement(1714..3174)
                     /gene="guaB"
                     /locus_tag="PK733_19185"
     CDS             complement(1714..3174)
                     /gene="guaB"
                     /locus_tag="PK733_19185"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOJ12694.1"
                     /translation="MNDRFEKEGLTFDDVLLVPQKSEILPKDIDTSTFLTRGIKLNMP
                     LLSAAMDTVTESRLAIAIAREGGIGIIHKNMSINEQATEVDKVKRSEHGVIVDPFYLS
                     PNHFIYDALELMEKYRISGVPITENGKLVGILTNRDLRFETDYSKKISEAMTKENLIT
                     APEGTTLTEAKEILRKHKIEKLPIVDKNGYLKGLITIKDIEKAIQYPNSAKDSNGRLL
                     AGAAVGITRDMMERVEALIYAKVDVIVIDSAHGHSANIINGIKLIKEKYPHIPVIAGN
                     VATAEGTRDLIEAGADAVKVGIGPGSICTTRVVAGIGVPQITAIYDCSSVAKQYGIPI
                     IADGGIKYSGDVVKAIAAGADTVMLGNMFAGTEESPGESEIYQGRRFKVYRGMGSLGA
                     MQKGSKDRYFQEDANKLVPEGVEGRVPYKGPISDTIFQLIEGLKHGMGYCGTGTIEEL
                     KTNSKFIRITNAGLKESHPHNIFITKEAPNYSTINV"
BASE COUNT          974 a          713 c          477 g         1237 t
ORIGIN      
        1 tataaccttt gcatttttca gcattgtttc tatttgaaca atttttgtct ctacatatgc
       61 ctgctcattc ttagcttcat catattcgga attctccgat atatctccaa atgatagagc
      121 ttgctttatt cgttctgcaa tttcccttct ttttgttatc tttaaaaatt caagctcctt
      181 ttcaagtttc tccaagccct cataagttaa tataacttct ttgcctgtca taagaagact
      241 ctcctttact aattgatttt gtgtgttgat aatatatgca ttaaagcctt atttattccg
      301 ttttcagtat ttaacagtat ataaacataa acagcactga taatataaac agtgcctttg
      361 tcaaaatccc ccagcttttc tataactttt ttaattttat ccaagttctg tgttaatttc
      421 atttaattat aaaatacaaa gtctatattg tcaagaagtt tacctggctt gggtacattt
      481 atcaagtctg aagaaatata ttcaagaagc tggctttatg tctatggtat taaagccggt
      541 aaagccctta atataatatc catcactctt aaagcgtttt gccatttcct cctgaaccca
      601 tccggtacgg aaccctgtta atatttctgc tgctacggcg gaagtgaact gctcaggtat
      661 taattcccga aatttatcag gaatagcttt agaaatattc gttacaatac cttctatcag
      721 tggatttttt aatcctggtt ttttaaagga aatattcaag tgggtaaaac tgttatttac
      781 tattacagtt tctgccttta acttggagca agttacaaga ttttgcacac aactttctac
      841 gcaaccataa tttaatatta tatctgcgtt tcttaaagca ttatatgctc cgccgcccaa
      901 gttgcaagct aaatttacag caagccctgt ggctgcataa atatcttcaa gttcattttt
      961 cagccatata acatcttcat catataacaa cacagtcaaa tatttaatca caagagaaag
     1021 ctttcttata gtttttagaa cttcctcctt atcttctaca tcaccaatta ttacaacatc
     1081 tgattgtctt atatcaattt tcagatcatt acaaattttt ttaagtatat ccagagtcag
     1141 ggcacggaaa agaaccttcc caggatacat tatttctttt ttgctgcgat tatgggatgt
     1201 ctgttttacc tgtgctgtct ggaatttata agtttcttgc aaaacctgta tttcaaaacc
     1261 gggtattgca aatagtttat ttgaaaaata acacctgtct gtttcttttt caaagcataa
     1321 actgccgatt ttctctaaaa tactattttt aatgtcagct gctctgtctt tacctttcca
     1381 actgtaaaaa tcaggttcta aaacataata tggcaattca gttaaaaata tgttcccatt
     1441 actacttgat tctatatcca tgaggctttt gttaatatac tcagtaatcc tctttttgta
     1501 aaatttttta tctaataaat atgaaatcct gctttgcaaa acacctgctc ttccttcgtt
     1561 acgctgtccg gctgcaacta ataaaatatt attcatgcat aatcctccgc cagaatattt
     1621 atcaatacta caagcaccat atagtattac tattcatgga aatgaagatt atgcatttaa
     1681 atcaaaggct tatttataca ttagaaagct tatctataca ttaattgtac tgtaattagg
     1741 agcttccttt gtaataaata tattatgtgg atggctttct ttaagtcctg cattagttat
     1801 tcgtataaac tttgaattgg ttttcagttc ttcaattgtt cctgtaccac aataacccat
     1861 cccatgtttt aaaccttcta taagctggaa tattgtatcg gaaatgggtc ctttataggg
     1921 aactctacct tcaactcctt caggaaccag tttgtttgca tcctcctgga aatacctgtc
     1981 cttgctgcct ttctgcatag cgcctagaga acccataccc ctgtatacct taaaccgtct
     2041 cccctggtaa atttccgatt ctcccgggct ttcttcagta cctgcaaaca tgttgccgag
     2101 cattactgta tcagcgcctg cagcaatggc tttaacaaca tctcctgaat acttgatacc
     2161 accatcagca attattggta ttccatactg ttttgcaaca cttgaacagt catatatagc
     2221 tgttatttga ggtactccta ttcccgcaac cactctagtt gtacatatag aacccggccc
     2281 aatacctacc tttacggcat ctgcacctgc ttctattaaa tctcttgttc cttccgcagt
     2341 agcaacattg cctgcaataa caggaatatg gggatatttt tctttaataa gttttatgcc
     2401 attgataata tttgcagaat gcccatgagc tgaatctata actattacat caacttttgc
     2461 atatatcagg gcttctactc tttccatcat atctcttgta attcctacgg ccgctccggc
     2521 aagcagtctt ccattagagt cttttgcgga atttggatat tggatggctt tttctatatc
     2581 ctttattgta attaatccct taagataacc atttttatca actataggca gtttttctat
     2641 tttatgcttt cttaaaatct cttttgcctc agttaatgtt gtcccctctg gtgcagtaat
     2701 tagattttcc tttgtcatag cttcactgat ttttttactg tagtctgtct caaatcttaa
     2761 gtccctgttt gttaagatac caacaagctt accgttctcg gttattggaa cccctgatat
     2821 tctatatttc tccattaatt ccaaagcatc ataaataaag tggtttgggg ataaataaaa
     2881 agggtctaca ataaccccgt gttcagacct ctttacctta tctacttctg ttgcctgttc
     2941 attaatactc atgtttttgt gaattatacc gattccacct tctctagcta tggcaatagc
     3001 aagtctggat tcagttacag tatccatagc agcacttaac aaaggcatat tcaacttaat
     3061 accccttgta agaaaagttg aagtatctat atctttgggt agtatctctg atttttgcgg
     3121 tactaacaat acatcgtcga aagtaagtcc ttctttttca aatctatcat tcatatgcat
     3181 tcctcctcta acccttgcgg ggcgtaataa attaaaccgg acaaaaatat taggatatat
     3241 tatactagag aaggaccaat gcgtcaagga attaaatcaa ttcttgccat atttcccttc
     3301 aaatacagaa aatgcaccta atacacggca atacattccc ttattcctct atcaactcac
     3361 ttataggtgc tccgggcggt actataggaa aaactttctc g
//
LOCUS       DAOSDD010000370         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9932_length_3375_cov_3.575301, whole genome shotgun sequence.
ACCESSION   DAOSDD010000370 DAOSDD010000000
VERSION     DAOSDD010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9932_length_3375_cov_3.575301"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK733_19190"
     CDS             <1..181
                     /locus_tag="PK733_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12695.1"
                     /translation="QIIDYPKCIRVVYYVNIESLGVKSLYFEIQYCHLLVPMRAKAIR
                     PPALGHAPATSIQQV"
     gene            complement(479..2044)
                     /locus_tag="PK733_19195"
     CDS             complement(479..2044)
                     /locus_tag="PK733_19195"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOJ12696.1"
                     /translation="MSLNLVREIIKENRVASRESVQIIIENDIIVPDTKPDIGRILFA
                     DGDAYIPNITVDDGNISMDGVIRYKILYVEDNPGQNIIALNINTDFEHSMDVPDVGKG
                     MEGRVKCDVEHVDCQIVNGRKVNVKSILRVKSNITEENEYYFINDIEDNEDIQILRNS
                     VSINKFIGTSQDICTVKESFEVPSGNPSIREILRNDIKITDIECKITDNKIILKGDAN
                     IQTLYAGDDKEESIRFMEHEIPFTHVLDLPGINEESDIHSDYDIQNYSFEPLEDEDGE
                     FRFMNGEIQIKIWILGSAKEDIDVIADAYGLRMDLSLEKTKVSMEEFVVGNRSQAVLK
                     ETLTAENDYPEIIEIFNVFSRPVLSEYEVGENRICIEGIVNNHILYYSNSPEQPIFCW
                     NQDIPFKQTVEVQGVKPGMACEVNLEIEHSNYNMLSHREVEIRLVIGIASRFYDTVEV
                     SFIEKVLENPTGEGRVRQLRPSITLYFVQEEDSLWNIAKKYRTTIDDIIRENKIDDLD
                     SIGSGMQVLISRK"
     gene            complement(2360..2626)
                     /locus_tag="PK733_19200"
     CDS             complement(2360..2626)
                     /locus_tag="PK733_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOJ12697.1"
                     /translation="MAEKSDLVQIKRNIETCIGERVQLKSNKGRKKIFIREGVIENTY
                     PSIFTIRFENEYEMTRRVSYSYTDVLTNAVELVVCKNNRRIQVS"
     gene            complement(2806..>3375)
                     /gene="yabG"
                     /locus_tag="PK733_19205"
     CDS             complement(2806..>3375)
                     /gene="yabG"
                     /locus_tag="PK733_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOJ12698.1"
                     /translation="GFKRPGKVLHLDGDQDYLETCMKEYKKRGIDAIGKHVTEREQPS
                     YVYRLLQEHRPDILVLTGHDGISKDQKNYSNINSYINSRHFIEAVKEARKFNLDMDGL
                     VIFAGACQSMYDGILKAGANFASAPHRVLIHALDPVMVTEKLAFTSVDRVIMPMDVIN
                     NTITGLKGIGGLQTRGKFRNGYPKEPYNS"
BASE COUNT         1029 a          699 c          458 g         1189 t
ORIGIN      
        1 acaaataata gattatccaa agtgtattag ggttgtatat tacgtaaaca tcgaaagttt
       61 gggggttaaa tcattatact ttgaaatcca atactgccat ttactcgttc caatgcgtgc
      121 gaaagccatt aggcctcctg cattagggca tgcacctgca acatccatac agcaggtttg
      181 ataaacttga tgccgcattc gaacttcgca atgacaaaga atctgcaacc tcttgtatca
      241 taatgcagct catggctgtt cttcttgcgg tctgctttta caacttgtaa ttttcgagca
      301 gactcccggg ttgcaacgat gttgcaactc cccgcaggtt atcctgcagg aaaaagccgt
      361 gatacggctt tctgggaagc aatcatatta tgtgtcaaaa aactaaatgc acccgtaaat
      421 ggctcatatt tttattttat aaacttatta aactacttat taactattta ttaactactt
      481 atttacgcga tataagcacc tgcatgccgg agccgattga gtctaaatca tcaattttat
      541 tttctcttat aatatcatca attgtagttc tgtatttctt ggcaatattc catagggaat
      601 cttcttcctg tacaaaatac aaagttatgc ttggtcttaa ctgccttact cttccttcac
      661 cggtaggatt ttctaaaact ttttctataa aggatacttc tactgtatca taaaaccttg
      721 aagcaatacc aattaccaat ctaatttcaa cctccctatg agacaacatg ttgtagttac
      781 tatgctctat ttcaaggttg acttcgcatg ccatcccagg ttttactccc tgtacttcca
      841 ccgtctgttt aaaaggaata tcctgattcc aacaaaaaat aggctgttca ggagaatttg
      901 aatagtaaag tatatggttg ttaacaatac cttctatgca aattctgttt tctcctacct
      961 catattctga taaaacaggt ctgctaaaca cgttaaatat ttctattatt tcaggataat
     1021 cattttctgc tgttaaagtc tcttttagta cagcctggct cctatttccg actacaaact
     1081 cttccatact tacttttgtc ttttcaagac ttaaatccat ccttaatcca tatgcatcgg
     1141 caatcacatc gatatcttct ttagcacttc ccaaaatcca tatcttaatt tggatttccc
     1201 cattcataaa tctgaattca ccatcttcat cttccaatgg ttcaaaacta taattttgaa
     1261 tatcatagtc tgaatgaatg tctgattctt catttattcc tggcaagtca agaacatgag
     1321 taaaagggat ttcatgttcc ataaacctta tactttcttc tttatcatcc ccggcatata
     1381 gagtttgaat attggcatct ccctttagaa ttattttatt atcagttatt ttgcattcta
     1441 tatcagtaat ctttatatca ttccttaata tttcccttat ggaaggattg ccggaaggaa
     1501 cctcaaaaga ctctttaacg gtgcaaatat cctgacttgt accaataaat ttattaatac
     1561 taacactatt tctcaaaatt tgtatatctt cattatcttc aatatcgttt ataaagtaat
     1621 attcattttc ttctgttata ttgcttttta ctctaagtat cgactttaca ttaacttttc
     1681 ttccgttcac aatctggcaa tcaacatgct ctacatcgca tttaaccctg ccttccattc
     1741 ctttgccgac atccggtaca tccatgctat gttcaaagtc tgtatttatg tttagagcta
     1801 taatattctg tccagggtta tcttcaacat aaaggatttt ataccggata actccatcca
     1861 ttgatatatt tccgtcatca actgtaatat ttggaatata agcatccccg tcagcaaaca
     1921 aaattcttcc gatatcaggc tttgtatcag gcacaataat atcattttca ataattattt
     1981 gcacactttc acggcttgca actcgatttt cctttattat ttccctaacc aaattaagtg
     2041 acatcctaat ccacctctac ttataagaat attttaacaa aaatcttact atgtaatata
     2101 tattgttaag actaagaaaa tattacaaaa catccggctg aaagcccatg actttattta
     2161 atgaggtgag atacgggaat tgagaaatga gaaaccggat gtaaaaaaca gatacggcat
     2221 ctgtttcgta aatgcagaat gccagtttat gtatacaaaa aagcaaccgg atttaactta
     2281 cggttgctca tttgtataca taagtcaaaa ttatttcact tcttttggta gttaactgtt
     2341 gaccttacct aatctgatat tagctgacct ggattctacg gttattctta caaacaacaa
     2401 gctcaacagc atttgtcaaa acatcagtat agctatagga aactcttctg gtcatttcat
     2461 attcattttc aaacctgata gtgaaaatac ttgggtaggt attttcaata acaccctctc
     2521 ttataaaaat cttcttcctt cccttatttg attttaattg cactctttca ccaatacagg
     2581 tttcaatgtt tctctttatc tgaacaagat cacttttttc agccatcaca tcacctcatc
     2641 attagtttgt taatattata tcatattaga tggcaattgt caaaggaaaa ttaaatatta
     2701 taacaattta caatagctta tgtcaagtac tatatacgca caaaattagt aaataaacat
     2761 aaataaattt aaaataatag ggttattcgc cgcaaaatcg cctttctagc tattatacgg
     2821 ttctttagga taaccatttc taaatttacc tcttgtttga aggccaccta tacccttcaa
     2881 cccggttatt gtattgttta ttacatccat gggcattatc accctatcta cagatgtaaa
     2941 tgcaagtttt tcagttacca taaccggatc aagagcatgt ataagaactc tatggggcgc
     3001 gctagcaaaa ttagctcctg cttttaatat accatcatac atagactggc aggcccctgc
     3061 aaaaataacc agcccatcca tatcaagatt aaattttctt gcttctttta cagcttctat
     3121 aaagtgtctc gaattaatat aactatttat gttggagtaa tttttctgat ctttcgaaat
     3181 accatcatga cctgttaaaa caagaatatc aggcctgtgt tcttgaagaa gcctatacac
     3241 ataagagggc tgttctcttt ctgtaacatg ctttccgata gcatctatcc cgcgtttttt
     3301 gtattctttc atacaagtct caagataatc ttgatcccca tctaaatgca gtacttttcc
     3361 cggtctttta aagcc
//
LOCUS       DAOSDD010000371         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_9960_length_3366_cov_3.289641, whole genome shotgun sequence.
ACCESSION   DAOSDD010000371 DAOSDD010000000
VERSION     DAOSDD010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9960_length_3366_cov_3.289641"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="nth"
                     /locus_tag="PK733_19210"
     CDS             complement(<1..544)
                     /gene="nth"
                     /locus_tag="PK733_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ12699.1"
                     /translation="MTKRQRVIKIIGIFDRLYPEAGCSLEYKDPLQLLIAAQLSAQCT
                     DARVNIVTKTLFEKYKNVYDFAGADLEELQEDIRSTGFYRNKAKNIKNCCRMIIDEFD
                     GMIPDNMDNMLMLPGVGRKTANLILGDIFGIPGIVVDTHAKRLSKRIGLTENDNPEKI
                     EYDLIKVVPKAEWTRFCHQLV"
     gene            complement(623..2125)
                     /gene="glgA"
                     /locus_tag="PK733_19215"
     CDS             complement(623..2125)
                     /gene="glgA"
                     /locus_tag="PK733_19215"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOJ12700.1"
                     /translation="MQSEPKLKILFVSAEVSPFAKTGGLADVAGSLPKALAQMGNDVR
                     IVMPRYRDIVSSMKYVTDFSVHMDNNAKTCIIRETEITAGTAEGRMNIPVYFIDSYDY
                     YDRDGIYCHPDDGERFAFFCNSTIEMLPKINFQPDIIHCNDWHTGPICMLLKEKYIKH
                     NFYKGISTLYTIHNLEYQGNFPEEILKLFNIGYEIFVPDKVEFYGMFNFMKAGLVYAD
                     IINTVSETYSKEIQTQQYGEGLDGLLIARDADLFGIVNGISYEEFDPSTDKRIYRNYD
                     SMLVENKKENKYALQKETGLPVGDMPVIGLISRLSGQKGLELIIDRIDEIMKHNLQFI
                     LLGTGDPYYESSFLKIKNKYMDKMAVYIEFNAVLAQRIYAGSDMFLMPSRFEPCGLGQ
                     IISLRYGTIPIVRATGGLSETITDYYRDKDRGNGFSFDEFSSDEFLKTMEGALMLYNQ
                     RPEEWRKLVKRALEQDFSWRRSAEKYVKLYNAAIKRKKYPIGIKQIIKPL"
     gene            complement(2403..>3366)
                     /locus_tag="PK733_19220"
     CDS             complement(2403..>3366)
                     /locus_tag="PK733_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ12701.1"
                     /translation="TWIAKKVSPSIVGIKVTARVQNFFFGEQEDKGEGSGIIIREDGY
                     ILTNYHVISKAYDSTTNKISSNAKIEVFLPEQEEKPYTAEIIGVDWRTDIAVLKIKGS
                     GFQAAELGDSDIVEVGELAVAIGNAGGYMGSVTAGVISGLNRTIQSDSGYEFKLIQTD
                     AAINPGNSGGALVNSEGKVIGVNSIKVVAQGFEGIGFAIPINTAREIFENLIEHKYVP
                     GRPYIGFKADITFDEAAAKYYRVPSGVLVEQVFPFTAAYKAGIYAGDIITKCQDKPVK
                     TLEELNEIKNQYKPGDTIKLEIYRDRKYITIEVELTEEKPNFEE"
BASE COUNT          953 a          736 c          471 g         1206 t
ORIGIN      
        1 aaacaagctg gtggcaaaac cttgtccatt ccgctttggg aactaccttt attaaatcgt
       61 actcaatctt ttccggatta tcattttctg taaggccaat tcttttcgat aaccgttttg
      121 catgggtatc aacaactata cccggtatgc caaatatatc accaagaatt aaattagccg
      181 tctttctgcc aactccggga agcattagca tgttgtccat attgtcaggt atcatcccgt
      241 caaattcatc aataatcatt ctgcagcagt tttttatatt ttttgccttg ttcctataaa
      301 atcctgtaga tctgatatct tcctggagtt cttccaaatc agctccagca aaatcataaa
      361 cgtttttata tttttcaaac aaggtttttg ttacaatatt tactcttgca tcagtgcatt
      421 gtgcggacag ttgagcagct ataagcagtt gaagagggtc tttgtattcc aaagagcacc
      481 ctgcttcagg gtataatctg tcaaatatgc ctataatttt aataaccctc tgtcttttag
      541 tcatttccat tgttcctcct caaaggtcat aacctgtttt ttcttcttcg tttttaatga
      601 tatcaattca aatcatatca tttcacaacg gttttataat ttgtttaata ccaattgggt
      661 atttcttacg ttttatagca gcattataca atttcacata tttttctgca gaccttctcc
      721 atgaaaaatc ctgttctaat gctcttttaa caagcttccg ccattcttcc ggcctttggt
      781 tataaagcat taatgctcct tccattgttt tcaaaaactc atcagatgaa aactcatcaa
      841 aggaaaaacc atttcctcta tctttatctc tataatagtc ggtaattgtt tcagaaaggc
      901 cacctgttgc ccttacaata ggaattgtac catatcttag acttattatt tgtccaagtc
      961 cacagggttc aaaacgtgag ggcattaaga acatatcact tcccgcatat attctttgtg
     1021 ctagaacagc attaaactct atataaactg ccattttatc catgtattta ttctttatct
     1081 ttaaaaagct actttcataa tatgggtctc ccgtacccag aagtataaat tgaaggttgt
     1141 gtttcattat ttcatcaatt ctatcgatta tcagctcaag gcctttttgc ccggaaagtc
     1201 ttgatatcaa tcctattaca ggcatatctc ccacgggaag tcctgtttct ttctgcagag
     1261 catatttatt ctctttctta ttttcaacca acatactatc ataattccga tagattcttt
     1321 tatctgtaga aggatcaaat tcttcataac taatgccatt aacaataccg aacaaatctg
     1381 catctcttgc tattaacagc ccgtccaacc cttcaccata ttgttgtgtc tgtatttctt
     1441 ttgaataagt ttcgcttaca gtattaataa tatccgcata tacaagtcct gccttcataa
     1501 agttaaacat cccataaaat tctactttat cgggaacaaa tatttcataa ccaatgttga
     1561 atagttttaa tatttcttcc gggaaattac cctgatattc aagattgtgt attgtatata
     1621 atgtggaaat ccctttgtaa aaattgtgtt ttatatattt ttcctttagc aacatacata
     1681 ttggtcctgt atgccagtca ttgcaatgta tgatatctgg ctggaaatta attttgggga
     1741 gcatctcaat ggttgaattg cagaaaaagg caaatctttc cccatcatcc ggatggcagt
     1801 atattccgtc tctatcataa taatcatagc tgtcaataaa atatacagga atattcatcc
     1861 ttccctctgc tgtaccggca gttatttctg tttctcttat aatacatgtc tttgcattat
     1921 tgtccatatg gacagaaaaa tctgtaacat atttcatact gctgactata tccctgtacc
     1981 tgggcataac tattctcaca tcatttccca tttgcgcaag tgctttagga agagaacctg
     2041 ccacatctgc aagaccccct gtttttgcaa atggagacac ttctgctgaa acaaataaga
     2101 ttttcaactt gggctctgat tgcatttatt atcctccatt actataatta atacactttc
     2161 atatttgaat tcacttacaa taacttatac aaataactta agcttaatat tagtgtaaaa
     2221 acccattcag cccgtatata cattaccctt aagaaccgcg ccgacacttc ctatcataat
     2281 ataacctagc tactatttca acatgattta aatggaagta aaaagggctc tacccttaac
     2341 aacgtaggga gagccacaat ataatcagct ataatgctcc atttactctt aaatattgtc
     2401 cattattctt caaaattggg tttctcttct gttaattcta cctcaatagt aatatatttt
     2461 ctatccctgt atatttccag ttttatagta tcaccaggtt tatattggtt tttgatctca
     2521 tttagttcct caagtgtctt gacaggttta tcctgacatt tggtaatgat gtctcctgcg
     2581 taaatacccg ctttatatgc cgcggtaaat ggaaacacct gttcaacaag tactcccgaa
     2641 ggaactctgt agtattttgc tgctgcttca tcaaacgtta tatctgcttt aaaacctata
     2701 taaggtcttc ctggaacata tttatgctca atcagattct caaatatttc tcttgcagtg
     2761 tttattggta ttgcgaaacc tatcccctcg aacccttggg ctacaacctt aatgctgttt
     2821 acgcctatta ccttgccttc ggaattaaca agagctccac cgctgttgcc ggggtttatt
     2881 gctgcatcag tctgtatcag tttgaattca taaccactat cagattgtat ggttctattc
     2941 aatccactta ttactcctgc tgttaccgaa cccatatacc cgcctgcatt gcctattgca
     3001 acagcaagct cacctacttc cacaatatct gagtcaccta attcagccgc ttggaaacca
     3061 cttcctttaa ttttcaatac agcaatatct gttctccagt ctacaccaat aatttcagca
     3121 gtatatggtt tttcttcctg ctctggtaaa aatacttcaa ttttagcgtt gctgcttatt
     3181 ttatttgtcg ttgaatcata cgccttcgat attacatggt agtttgtcag aatataacca
     3241 tcttccctaa taattatacc ggagccttca cccttatctt cctgttctcc aaaaaagaaa
     3301 ttctgaactc ttgctgtaac ttttattcct acaatagatg ggctaacctt cttagctatc
     3361 caggtt
//
LOCUS       DAOSDD010000372         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10004_length_3356_cov_5.112087, whole genome shotgun sequence.
ACCESSION   DAOSDD010000372 DAOSDD010000000
VERSION     DAOSDD010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10004_length_3356_cov_5.112087"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..217
                     /locus_tag="PK733_19225"
     CDS             2..217
                     /locus_tag="PK733_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ12702.1"
                     /translation="MAAVFIHKASNYKSSIWIEKDERKANAKSLLGLLSLGIGEGDKV
                     ILTAEGEDSEKAIKDLEEYLVSELGEE"
     gene            228..2153
                     /gene="uvrC"
                     /locus_tag="PK733_19230"
     CDS             228..2153
                     /gene="uvrC"
                     /locus_tag="PK733_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOJ12703.1"
                     /translation="MTVFDINEELKKLPEKPGVYIMKNTEGEIIYVGKAVNLKNRVRQ
                     YFQSLSGQTQKVRLMVPQISTFEYIITSSEMEALILECNLIKRYKPKFNILLKDDKTY
                     PYIKVTVNEEYPRVFMTRKVEKDGARYFGPYTSVSDVRETISFLKKTFPIKNCKKKLP
                     DDIGKGRPCLNYYIQQCMAPCQGWVSNGKYKEIINDICSFLSGKEDELLGKLQKEMSD
                     ASENMEFERAAAIRNKIISLKNMFQEQKVVSESPDDEDVIALARNEEFACVQIFFIRN
                     GKLIGRESFIFTNIEESNSELMESFIKQFYSSSEYIPKYILLQVDIYEKELFENWLQE
                     KRGSKVHIRVPRKGEKLKLVKMVSQNAQTYLNNYVQTGEDQKKPYLIALDELAKLMGT
                     VKIPARIEAYDISNTGSTEITGSMVVFEDGISSPKQYRRFKIKSLNQPDDYEAMQEMI
                     FRRFRRGRLERVDDKDHTGMENEENSIKVTQSSVEKYAKPSDGFSIYPDLILVDGGIG
                     HVNAIIEVLEHSGLQIPVYGMVKDEKHRTRGLILPGKEINLSTNQILLKFITEIQNEA
                     HRFALEYNKKLRNKRYTKSVLDDIPGIGSGRKKDLIRHFGSVDKIRRAEVDDLMDVRG
                     INKTLAINIYEFFRRGK"
     gene            2199..2927
                     /locus_tag="PK733_19235"
     CDS             2199..2927
                     /locus_tag="PK733_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ12704.1"
                     /translation="MAVYAISDLHLALGVDKPMDVFGGRWLNYMERLRHSWRETVTAD
                     DYVLIPGDISWATYLEEAYKDFKYIDDLPGEKIILKGNHDYWWTTLRKLDIFLKNNKL
                     FSINFLHNNSYITDDLIICGTRGWKCPGDDDFSEDDSKIYTRELQRLQLSLESSAKIN
                     SNTAAAGERNIIVALHYMPFSSRREPSGFIDLMQKYNVKMCIYGHLHGEGAWKNAVNG
                     EIEGITYRLVAADYLDFKPVRIQV"
BASE COUNT         1242 a          426 c          726 g          962 t
ORIGIN      
        1 aatggcagct gtttttattc ataaagcttc aaattataaa tctagtatct ggattgaaaa
       61 agatgaaaga aaagcaaatg caaagagtct gctggggctt ttatccctgg ggataggtga
      121 aggtgataaa gtaatactta cagcggaagg agaagattcg gaaaaggcaa taaaggatct
      181 ggaagagtat ctggtttccg agttggggga ggaatgataa tattaacctg acggttttcg
      241 atataaatga ggaattgaaa aaactacctg aaaagccagg agtttatatt atgaaaaata
      301 cagaaggtga aataatttat gtagggaaag cagtaaacct taaaaacaga gtaagacagt
      361 attttcagtc tctgtcaggg caaactcaaa aagtccggct tatggttcct caaataagta
      421 cctttgaata tataattaca tcttctgaaa tggaagccct cattttggaa tgcaatttaa
      481 taaagagata taaaccgaag tttaatattt tattgaaaga tgacaagaca tatccttata
      541 taaaggttac ggttaatgaa gaatacccga gagtttttat gaccagaaag gttgaaaagg
      601 acggggccag atattttggg ccgtacacaa gtgtttccga tgttagggaa acaataagtt
      661 ttttgaagaa gacatttccc ataaagaatt gtaaaaagaa gctccctgat gatataggaa
      721 aaggaaggcc atgcttgaac tattatatcc agcagtgcat ggcaccttgt cagggatggg
      781 tttcaaacgg gaaatacaag gaaataatta atgatatttg cagcttttta tcaggtaaag
      841 aggatgaatt actgggaaag cttcaaaagg aaatgtctga tgcttctgaa aacatggaat
      901 ttgaaagggc tgcggctatt agaaacaaaa tcattagttt gaaaaatatg tttcaagagc
      961 aaaaagttgt atccgaatct cctgatgatg aagatgtaat agcattagca agaaatgaag
     1021 aatttgcctg tgtacagata ttctttataa ggaatggcaa gctcattggc cgggagagtt
     1081 ttattttcac aaatattgag gaaagcaaca gtgagcttat ggaatctttt ataaagcagt
     1141 tttacagttc ttcagaatat attccgaaat atattctact tcaagtggat atatatgaaa
     1201 aggagttatt tgaaaattgg ctgcaggaaa aacggggatc aaaagttcat attagagttc
     1261 ccaggaaagg cgaaaaatta aaacttgtaa aaatggtttc tcaaaatgcc caaacatatc
     1321 taaataatta tgttcaaaca ggggaggatc aaaagaagcc ttatttaatt gcccttgatg
     1381 aactggcaaa gcttatgggc actgtaaaaa tacctgcaag aatagaagca tatgatattt
     1441 caaacaccgg aagtactgag attactggat ctatggttgt atttgaggat ggtatttctt
     1501 cacctaaaca atatcgaaga tttaaaataa agtctctaaa ccagcccgat gattatgaag
     1561 ctatgcagga aatgatattc agaaggttca gacgtggcag gcttgagaga gttgatgaca
     1621 aagaccatac aggtatggaa aacgaggaaa atagtataaa agttacccag tccagcgtag
     1681 aaaaatatgc aaaaccaagt gatggttttt ccatatatcc tgacctgatt cttgtagatg
     1741 gaggaatagg gcatgtaaat gcaataattg aagtattgga acattctggt ttgcaaatac
     1801 ctgtatatgg aatggtaaaa gatgaaaaac acaggacacg gggacttata ttgcccggga
     1861 aagaaataaa tttaagcact aatcagatat tgttgaagtt tataactgaa attcagaatg
     1921 aggctcatag atttgcattg gagtataata aaaaattaag aaataaaagg tatacgaaat
     1981 ctgttctaga tgacatacca ggaattggaa gcgggagaaa aaaagatctg atacggcatt
     2041 ttggttctgt agacaagatc agaagagccg aagtcgatga tttaatggat gtaaggggga
     2101 taaataaaac tcttgcaata aatatatatg aatttttcag gcggggaaaa tagaattgcg
     2161 gaagagagta gagacttatt cgggagagga tagaataaat ggcagtatat gcaatttcag
     2221 accttcatct tgcattaggg gttgataaac ctatggatgt ttttggcggc aggtggttga
     2281 attatatgga aagattgcgg cattcatgga gagaaacagt gaccgctgat gattatgtac
     2341 ttattcccgg ggatatatcc tgggctacat atctggaaga ggcgtataag gattttaaat
     2401 atattgatga tcttccaggg gaaaaaataa tccttaaagg aaaccatgat tattggtgga
     2461 caacattaag gaagcttgac atttttctga aaaacaataa acttttttcc ataaatttct
     2521 tacataataa cagttatata acggatgatt tgattatttg cgggacaaga ggctggaaat
     2581 gccctggaga tgatgatttt agtgaggatg acagtaaaat atacactaga gaattacagc
     2641 ggcttcaact ttcacttgaa agttctgcaa aaataaattc caatacagct gcagctggcg
     2701 aaagaaatat tatagtagct ttgcattata tgccttttag ctctagacgg gaaccatccg
     2761 gttttataga cttgatgcag aaatacaatg tgaaaatgtg tatttatgga catctgcatg
     2821 gggagggagc ttggaaaaat gctgttaacg gtgaaataga aggcataaca taccggctgg
     2881 tagcagcaga ctatctggac tttaaaccag taaggatcca ggtttagtgg taatattagt
     2941 cagtttataa cttttaaaaa atgcgctgca attttcacat ctttgtttac agctaagata
     3001 tggtatgtta taatgtataa gttaattaag aaccatatgt ttttaatggc atatataacc
     3061 ttttaaaggg attaaatagt atttgcctat tagaacttgt gtaacatgga aactttatgt
     3121 tatgcttcta atatgagcta tttgaatcct tatatttatg tgtatatgtt taatagaggt
     3181 gggaaatggg aaattaaaag ttagagatga gaaatgagat ataatatgca acacatagta
     3241 ttaataaaat atttaataaa atatttaata aaatagttga tgaagcagct gataaatatg
     3301 aaagctcatt aaaataaata aaaaaataat aataaacaga taagtatgga taaaac
//
LOCUS       DAOSDD010000373         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10138_length_3324_cov_3.115938, whole genome shotgun sequence.
ACCESSION   DAOSDD010000373 DAOSDD010000000
VERSION     DAOSDD010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10138_length_3324_cov_3.115938"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PK733_19240"
     CDS             complement(<1..940)
                     /locus_tag="PK733_19240"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12705.1"
                     /translation="MKLIIIGTGSRGGVYARYVQEYGAEIVAVADPNEERLSNFTDEY
                     NIPQNGLYKSWEEVFAVEKFADAVIISTPDKVHYDPAMAALEKGYHVLLEKPMSSKEQ
                     ECRDMVNKSREKGLVLMVCHVLRYAPFYEKLKEIIDEGRIGRIVNLQHTENVAYWHFA
                     HSFVRGIFRNETISSPWILAKSCHDLDIITYLMDKKCKSVVSKGNLMYFTKENTPEGA
                     PEYCLDNCPHEKTCQYFAPALYLKQITNVTWPSDVISIDTSFASRYEALKKGPYGRCV
                     YRCDNDMVDHQSAIFEMEDGSTAAFNMIGFSSENTRT"
     gene            1495..2709
                     /locus_tag="PK733_19245"
     CDS             1495..2709
                     /locus_tag="PK733_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ12706.1"
                     /translation="MSTTIDLRKKNTLLTLKTIMEGDSITKADVANKTGLTLMTVNTI
                     MNSLMKKAIITDCGVADSSSGRKATLYTVNPAAHNIIGVNIDVGTITFAVSDFNLRIR
                     EAIVIETNNNTSPEETIDLILKTIVGLIKNHCIKPASILGVGVTVPGPVDEISGVIHS
                     FPNLKGWANIPLKDVLKEKLDIPVFVEKDNYASVLYLKRNLDNTYKNVVSLTIKGGIG
                     TGILLGGRLFRGENGIAGEIGHVSVDMDGPRCNCGNFGCLEIYASDYAIIKNVKNSIE
                     KGVKSGILCVNDDEIDHLDIDKIIEAALNGDELCRDTILKAAEYVGLAVSNAIKFYDP
                     GYFIINSRWIKEIKEAHSIITDITKERCTLVQNDKINLAFFYEKDVYLMGALKLVREH
                     VIANTYNNRLIS"
     gene            2996..>3324
                     /locus_tag="PK733_19250"
     CDS             2996..>3324
                     /locus_tag="PK733_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ12707.1"
                     /translation="MNILIDTQKYTERVSNALKASLFIELESVASILLEARKNNRWIF
                     LAGNGGSASTASHFANDMMKGLSIIGKPRFRAKALCEAMPIITALANDYNYDLIYVEQ
                     LKNYASTG"
BASE COUNT         1109 a          560 c          610 g         1045 t
ORIGIN      
        1 tagttcttgt attttcactg gaaaaaccta tcatgttaaa cgctgctgta gaaccatctt
       61 ccatttcaaa tattgcactt tggtgatcca ccatatcatt atcacacctg tatacgcatc
      121 ttccataggg gccctttttc agggcttcat atctgctagc gaaggatgta tcaatagata
      181 taacatcaga tggccaggtt acattggtta tctgctttaa gtataaggca ggtgcaaaat
      241 attggcaggt tttttcatga ggacaattat caagacaata ttccggagca ccttcaggag
      301 tattctcctt tgtaaaatac ataagattgc cttttgaaac gacagatttg cattttttat
      361 ccattaaata tgtaattata tcaagatcat gacaggactt tgccaatatc cacggacttg
      421 aaatagtttc attcctgaat atgcctctca caaaagaatg ggcaaaatgc cagtaggcta
      481 cattttctgt atgctgtaaa ttaacaattc gccctattct cccctcatca attatttctt
      541 tcaatttttc gtagaaaggt gcataacgca acacatggca taccataaga acaagacctt
      601 tttctcttga cttattaacc atatcccgac attcctgttc ctttgatgac atgggctttt
      661 caagcaacac atgataacct ttttcaagag cagccattgc aggatcatag tgaaccttat
      721 caggtgtaga tattattact gcatcagcaa atttttcaac tgcaaaaacc tcttcccatg
      781 atttatataa accattttgt ggtatattgt attcatctgt aaaatttgac agtcgctctt
      841 cattagggtc agctactgca actatttctg caccatactc ctgtacatat cgggcataaa
      901 ctcctcctcg tgatcctgta cctattatta tcaatttcat agcaataaat atagggagcg
      961 ttaacttatg ttagatgcaa accagctgtg agatgtaaaa ttcaggctat actgaaaaac
     1021 agcaccctat ctcctttcct gttttttatt ttagattata tcaaatatga ttatttgtat
     1081 gcaatacaaa agctattttt aggagcattt ataaattgta actttaaatg tcgtacattt
     1141 aatggaaacc ctgtataaca ttacacatta cttaatatca gccacgcatc ctattgggtg
     1201 tcaagggtga gcgtagtgaa tttatcttga cccttgacgt ccagtataac atatatcctc
     1261 ttgcgtgtct gtctgtcata acaggccgac tgccttccgg cgaggacagg gtctgggaca
     1321 gcgtcccagg ataatcttat taaactgcta aatcattttc cgatttatat ccaaatattt
     1381 ttttttgggt atttacattc acaaatttta tggtacaatt aaataaaaga catttataaa
     1441 cgacttttat tataattact tattagcatt tatctttacc agaggagtaa ccctatgagc
     1501 acaacaattg acctgaggaa aaaaaataca ctgcttacat taaagacgat tatggaaggc
     1561 gattcaatta ctaaggcaga tgtagcgaac aagacaggtt tgacgcttat gactgtgaat
     1621 acaattatga acagcttgat gaaaaaggca atcataacag actgcggtgt tgccgattca
     1681 agcagcggta gaaaggcaac cctttatact gtcaatccgg ctgctcataa tataataggc
     1741 gtaaatattg atgtaggcac gataactttt gctgtttctg atttcaatct tagaatcagg
     1801 gaagcaattg taatagaaac aaacaacaat acatctccgg aagaaactat tgatctaata
     1861 ctgaaaacaa tagtgggatt aataaaaaac cattgcatta aacctgcaag cattttgggg
     1921 gtaggagtaa ctgtaccggg tccggtagat gaaataagcg gtgttataca ttcttttccc
     1981 aatttaaaag gttgggctaa tataccattg aaggatgtcc taaaagaaaa acttgacatt
     2041 ccggtctttg ttgagaagga caattatgca agtgtccttt atcttaagag aaatctggac
     2101 aatacatata aaaatgtagt atctttaaca ataaaaggtg gaattgggac agggatactt
     2161 cttggaggaa gacttttcag aggggaaaat ggtatagcgg gtgaaatagg acatgtttcg
     2221 gtggacatgg acggaccaag atgtaattgt ggtaactttg gatgtcttga aatatatgcg
     2281 tcagactatg ccataattaa gaatgttaag aatagtatcg aaaaaggagt aaaaagtggc
     2341 atattatgtg taaacgatga tgaaatagat catcttgata ttgacaagat tatcgaggct
     2401 gctttaaatg gggatgaatt atgccgtgat accatattga aagcagctga atatgtaggc
     2461 ttagctgtca gtaacgccat aaaattttat gatcccggtt attttataat aaacagcaga
     2521 tggattaaag aaataaaaga agcacacagt attataactg acattactaa ggagagatgc
     2581 acactggtgc aaaatgataa aataaatctg gcattctttt acgaaaaaga tgtatatttg
     2641 atgggtgcat taaagctggt tcgagaacat gtaatagcaa atacatataa caaccgtttg
     2701 attagttgaa tgttatttgt tatattctgt ttccaagaaa cttactatat taatcgcaat
     2761 aatgatattg catcagatga tttaattatt gcgatgtaat actaaggaaa ttgaatcacg
     2821 aaaacattac tgatgtagta tctttttcgc gttcaatata gttttttgtt tattggtaat
     2881 acgatatgtt aacaacaaaa ttagtaaaac caacatatta aggacagtct gtaggtaatt
     2941 aataaatatc ttttattaat atgtaaaatg ctaaagaaag tgaggtcaaa tatttatgaa
     3001 tattcttatt gacacccaga aatatacaga aagagttagc aatgctttaa aagcttctct
     3061 gttcatagag ttggagagtg ttgcaagtat cttgctggaa gcaagaaaaa acaaccgatg
     3121 gatttttctt gcaggcaatg gaggtagcgc tagtacagct tctcattttg ccaacgacat
     3181 gatgaaaggt ctttccataa tcggtaaacc cagattcaga gccaaagctt tgtgtgaggc
     3241 aatgcctatt attacagcac tggccaatga ctacaattat gatctgattt atgtagagca
     3301 attgaaaaat tacgcttcga cagg
//
LOCUS       DAOSDD010000374         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10247_length_3294_cov_4.907996, whole genome shotgun sequence.
ACCESSION   DAOSDD010000374 DAOSDD010000000
VERSION     DAOSDD010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10247_length_3294_cov_4.907996"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            675..1490
                     /locus_tag="PK733_19255"
     CDS             675..1490
                     /locus_tag="PK733_19255"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ12708.1"
                     /translation="MSTMNKIENVKEQYKDDNNLATRIKLHAKHSTNKQGLTPWLFEK
                     YSFSCGNRILELGCGNGEQWKNHIQQLPVDCILVLSDISEGMVKNVWEKYSTNKNLLV
                     QNIDIQNIPFPDNCFDVIIANHMLYHVPDLQKSLSEVKRILKVGGKFYAATNGNGGMR
                     LYLHNAFKQVNPESNFFTQKFSFNMQNGMEILRGYFDDVQKYDFEDSLSITETQDLID
                     WLKSTITIANYSENDLNGLYDYFERIRQREGSINIPKEAGLFISTKFLVLNVK"
     gene            1813..2775
                     /locus_tag="PK733_19260"
     CDS             1813..2775
                     /locus_tag="PK733_19260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ12709.1"
                     /translation="MSSSKLVVFLTNFYLQIILPFEMIHKIFTRGYDNKNEYILLVIY
                     VSVIFFFLFSYCYWEYTNFYLRYVYCFFIILGFFSIIYRVIFTQNIVFGELGDLNNRL
                     ETAIWIIVFIIIDVSIIFSKIKNKNCYTLAFPFRNGRFLVVDGGDGKKSFFTNYHYYG
                     WKNKKAINYYSMRFATDIIKLNKYGFANSKILSSSNEDYNIYGEKVYSPIEGEVVNMT
                     ANYDDRIPYAKLPNNGGNQITIKNGNYYISLYHFKKDTIVVNVGEKLKVGNYLGEVGN
                     SGYSPRPHLHIQVVYCEDNKHWLGKGVPIIFNNRYPVKNCSINA"
     gene            2907..3239
                     /locus_tag="PK733_19265"
     CDS             2907..3239
                     /locus_tag="PK733_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12710.1"
                     /translation="MNDNEEKLEWEENELEKNIADLKEWQDNQYNPGYYVGTGRIPRP
                     LKSLSRYPVILIIFGIIGLLTSAFIILNSKTSIASIPGFILGLFFSSCLVYGGIMRIK
                     ERNDKTKQ"
BASE COUNT         1161 a          453 c          647 g         1033 t
ORIGIN      
        1 tgaagtacat aaatctgctt tgaggcttcg ttacgacgtg ccggtgaaag ccggatacat
       61 agagaacaag acattcatct ggtactattt tctctcggag tatagcaggc acctaaatcc
      121 tgaacattaa agataagaag aatggaacac gggattgctt cataactgga gcctgacagg
      181 gcaaggaagg ttataaagta acacaggtac cgtagtagtc ggaggtatgg aaagcatact
      241 acatggcgaa gggtaccaga cgggaatata aaaattaaac ctaaaacagt gaagtctaac
      301 tcccattatg ggtagcgaac cgcaggttaa actgtgcact taaaacacaa cttcacgagc
      361 gaagcagact gaattaaggt ttaattgtgc ctgaaactgt tgtgagacag tagaaggttg
      421 aaagccgatg aaggcaagta agtgttcccg ttggaaagcc ggatgaattg aaaggttcct
      481 atccggtttg gagcagggga caaggtggag attacctcaa agccttacct attgctatgg
      541 ccgttagtac gatatatccc gcaaagccgc cgcatcctgc ggccgattgg ttctagggct
      601 gggcgttgta gtttttaata atgtgtaaca gttttaaaat actaaatgaa gattatttat
      661 ggaggtacat aataatgtct acaatgaata aaattgaaaa tgttaaagaa cagtataaag
      721 atgacaataa cttagcaaca aggattaaat tacatgcaaa acacagtaca aataaacaag
      781 gattaactcc gtggcttttt gaaaagtata gtttttcgtg tggtaatcgt atacttgaat
      841 tagggtgtgg caatggtgag caatggaaga atcatataca gcaactacca gttgattgta
      901 ttttggtttt gtcagatatt tcagaaggta tggttaaaaa tgtttgggaa aagtattcaa
      961 ccaataaaaa tttgttggta cagaatattg atattcaaaa cataccattt cctgataatt
     1021 gtttcgatgt tatcatagca aatcatatgt tatatcatgt tccagattta caaaagtctc
     1081 tttcagaagt aaaaagaata ttaaaggttg gtgggaaatt ttatgctgca acaaatggaa
     1141 atggcggtat gagactatat ttacataatg cttttaaaca ggtaaatcca gaaagcaatt
     1201 tcttcacaca aaagttttct tttaatatgc agaatggaat ggaaatttta agaggatact
     1261 ttgatgatgt tcaaaagtat gatttcgagg actcactttc aattaccgaa acacaggatt
     1321 taatagactg gctaaaatca acaattacta ttgcaaatta ttctgaaaac gatttaaacg
     1381 gcttgtatga ttattttgaa agaattagac aaagggaagg ttctattaac attccaaaag
     1441 aagctggact atttatttct accaaatttc tagtattgaa tgtaaaataa atggctcagg
     1501 agatattaca tgtaataagg caggtaatcc cacgatacaa ataaatggtt cgggagattt
     1561 taaatcaaag gaagtatgcg gaaacttgaa atcaaaaata agcggttcag gagatattgg
     1621 ccttaattca ggtcaactgg attcatttct tcaccgggca ggctttccat aacgttcctt
     1681 gtgcgtttca gagctgtcgc aagcacattc cgttatctga aatcggatgc agagtcggat
     1741 gcagagaacg tgtattcata agcatgagcg gacatcttat ccgcaaaaat agatagattg
     1801 tggggtgata atatgtcatc aagtaagcta gtagtttttt taacaaactt ttatttacag
     1861 attatactac catttgaaat gatacataaa atttttactc gaggatatga caataaaaat
     1921 gaatatattt tattagtaat atatgtgtcc gtgatattct tttttctttt ttcatactgt
     1981 tattgggaat atacaaactt ttacctaaga tatgtgtatt gcttcttcat aatacttggt
     2041 tttttctcga tcatttatag agttatattt actcaaaaca ttgtgtttgg tgaattaggt
     2101 gacttaaata accgactcga aacagctatt tggattatag tttttattat aattgatgtt
     2161 agtattatct ttagtaaaat caaaaataag aactgctata ctttggcttt tccctttaga
     2221 aatggtagat ttttagttgt tgatggtgga gacggcaaaa aatctttttt taccaattat
     2281 cattattatg ggtggaaaaa caaaaaagca attaattact attccatgcg ctttgctaca
     2341 gatataatca aattgaacaa atatggtttt gcaaatagta aaatactgtc ctcctctaat
     2401 gaggactaca atatatatgg agaaaaagta tatagcccta tagaaggtga ggtagtaaat
     2461 atgactgcaa attatgatga tcgtattcca tacgctaaat tacccaataa tggaggaaat
     2521 caaattacta ttaagaatgg taattattat atatctttgt atcattttaa aaaagacact
     2581 attgtagtaa atgttggaga aaagctaaag gtaggtaatt atcttggaga agtaggtaat
     2641 tctgggtatt cgccaagacc tcaccttcat attcaggttg tatattgtga agataataag
     2701 cattggttag gaaaaggtgt accaataata tttaataata gatatcctgt gaagaattgc
     2761 tcaattaatg cttagagtat tattaaagaa gttcccgtct atggttgctt ctgagttact
     2821 aaggataaac aaatgttctg gaacatattg caaatattaa tcacatatgg tacaatggtt
     2881 aacaagttga aaaggtgatt tattctatga atgataatga agaaaaatta gaatgggaag
     2941 aaaatgaact tgaaaagaat attgctgatt taaaagagtg gcaggataat cagtacaatc
     3001 caggttatta tgtaggaaca ggaagaattc caagaccatt aaaaagccta agtagatatc
     3061 ctgtaatact cataatattt ggtatcatag gtttgctgac aagtgcattt attattttaa
     3121 attcgaaaac ttcaattgct agcatacctg gttttatttt gggattgttc ttctcttcat
     3181 gtctagttta tggtgggatt atgagaataa aagaaagaaa tgataagact aaacaatagt
     3241 acgagcattc ctgtaatgaa gttgtatgat ctttgaaaaa aacagtaccc tctg
//
LOCUS       DAOSDD010000375         3287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10278_length_3287_cov_5.388614, whole genome shotgun sequence.
ACCESSION   DAOSDD010000375 DAOSDD010000000
VERSION     DAOSDD010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_3287_cov_5.388614"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..818)
                     /gene="hisF"
                     /locus_tag="PK733_19270"
     CDS             complement(57..818)
                     /gene="hisF"
                     /locus_tag="PK733_19270"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ12711.1"
                     /translation="MLTKRIIPCLDVHNGRVVKGINFVNLVDAGDPVACAMAYDKAGA
                     DELTFLDITATHDNRKTVADMVSRVAEQVFIPLTVGGGIRTVDDFRVILLAGADKIGV
                     NSAAVKRPELITEAAMRFGSQCVVVAIDAKKRAEGSGWDVYINGGRINTGLDAVEWAV
                     KAEKCGAGEILLTSMDRDGTKNGYDIELTRQVSEAVKIPVIASGGAGELKHFKEALTE
                     GKADAVLAASLFHFGEMNIMELKKYLSENGIQVRI"
     gene            complement(1095..2684)
                     /locus_tag="PK733_19275"
     CDS             complement(1095..2684)
                     /locus_tag="PK733_19275"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOJ12712.1"
                     /translation="MTKQQLEKRVNDLRTQLFNAFAKKEVMEESLKNRIYTLEQELAG
                     QAPAIEMLQKYIADCEMKINELKNENAELKKERAVFTATIEKMKMHIKKLLARLKKDS
                     TSSSKPPSTDVFHKPKPQNLREKSGKKPGGQLGHPGHGLKLFADPDTVIEKKVEVCDE
                     CGHSIINNQAFEAKQKIDIEIRTIVTEERVYEGVCSHCGKKVRGQFDKGFINPTQYGD
                     NIKATVALLSEHGCVSVNKTAEIINSLSGGRLALSWGTIVNMQRELSKKLEDTINVIR
                     TGLIAGNILCADETGCRVNGGLSWIQVFCNDRFVLFGLNSKRGDVDEQFGILTFFLGI
                     LIHDHFVSYYQFENVSHAECNGHILRYLKGLIEIFKHDWLKNMSTLLKSACHRKNELV
                     AAGATQMPIEDVAWFTSEYDRILEEGQQEYATATKGDKNMESYHVDERRLLTRLKEYK
                     EEHLRFLIDFQIPFTNNMAERDVRKFKNKVKVSGCFRSAEGAEIYARIASVITTLKKQ
                     KINVFNGLRAAYSGQVPISTA"
     gene            complement(2681..3139)
                     /locus_tag="PK733_19280"
     CDS             complement(2681..3139)
                     /locus_tag="PK733_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12713.1"
                     /translation="MKIDPPIKAKWFYMFASEKIKFNWFCYEYACMLYDHIRKSERLK
                     KWKSERSDLQVAEFCAYFAKRMKQAVLDKLNGLTDATTADEEYIADYCHENTHKQNLI
                     IMDIAGEAWDELLSSCEVCPNRCISERYARSEFFDRMEHGDYFSFNGDGK"
BASE COUNT          850 a          793 c          583 g         1061 t
ORIGIN      
        1 ataaaaagca cctgacaagc cagagtttgc aaggtgcaga cctattctaa ttgtatttaa
       61 attctgacct gaatcccatt ttcggaaagg tattttttta gctccataat attcatttcc
      121 ccgaaatgga acaacgaagc tgccaatacg gcatccgcct ttccctctgt taaggcttcc
      181 ttgaaatgct taagctcccc tgcacctcca gatgcaatta ccggtatctt aacggcctca
      241 gaaacctgcc gggtaagctc tatgtcatat ccgttttttg ttccgtcccg atccatactg
      301 gttaaaagta tttcccctgc accgcatttc tctgctttaa ctgcccattc cacggcgtca
      361 agaccggtat ttatccggcc tccgtttata taaacatccc atccggagcc ttcagccctc
      421 ttttttgcat caattgctac tactacacac tggctgccaa aacgcattgc tgcctcagtt
      481 attaattcag gccttttcac agcagcggaa tttacaccga tcttatccgc acccgccagg
      541 agaattactc tgaaatcatc aactgtacgt atccctccgc caaccgtcaa aggtataaaa
      601 acctgttcgg caactcttga caccatatct gcaacagtct ttctgttgtc gtgggtagct
      661 gttatgtcaa gaaaagtcag ctcatccgca ccggctttat cataagccat ggcacatgcg
      721 acaggatctc ccgcatccac cagattaaca aaatttatgc cctttacaac cctgccattg
      781 tggacatcaa ggcaaggtat gattcttttt gtcagcatag cacagccctt ctttcttcat
      841 cagatttatt ttaattgaat tataactttt ctgtttttgg ttttataacc ttaattatac
      901 ttcgattgat aatacccttg caagttcttt ctaataatat aattcatcgt aaataaaaag
      961 tgtgttacta ttcagaggta agtaatgttt agtaattaaa acaggaatat aagcatggga
     1021 aactataccc aataaaaaca aattgggtat cacaatttat tgttacaatt aaattgtatc
     1081 gagaaatggt tgtgctatgc tgtcgagatc ggaacctggc cagagtatgc agcacgcaga
     1141 ccgttaaata cattaatttt ttgctttttc agagttgtaa ttacacttgc tatccttgca
     1201 taaatctcag ctccctctgc acttctgaag caccctgaga ccttaacctt gtttttaaac
     1261 tttcgcacat cgcgttcagc catattattt gtgaaaggta tttggaagtc gatcagaaaa
     1321 cgcaagtgtt cttccttgta ttcttttaga cgggtcaaga gcctgcgctc atcaacgtga
     1381 taagattcca tattcttgtc accttttgtg gcagtcgcat actcttgctg cccctcttca
     1441 agaatgcggt catattcact tgtgaaccac gcaacatctt caatgggcat ctgcgtggca
     1501 ccagcggcaa caagttcatt tttcctgtgg caggctgatt tcagcagagt agacatgttt
     1561 ttaagccagt catgcttaaa aatttctatc aatcctttca ggtacctaag tatatgtcca
     1621 ttgcattcag catgggaaac gttctcgaat tgatagtagg atacgaaatg gtcgtggatg
     1681 aggattccca gaaaaaatgt cagaatacca aactgttcat ccacgtcccc gcgttttgaa
     1741 ttaaggccaa acaagacaaa gcgatcattg cagaaaacct gtatccaact caggccgcca
     1801 ttcacccggc aaccagtttc atcagcgcac agaatattgc ctgcaattag tccggtacgt
     1861 ataacattga ttgtatcttc aagctttttt gacagttcac gctgcatgtt cacgatcgtt
     1921 ccccatgaca gagccagtct tccaccactc agactgttga taatctctgc tgtcttgttg
     1981 acggacacgc aaccatgctc actaagaagc gcaaccgtcg ctttgatgtt atcaccatac
     2041 tgagttgggt tgatgaatcc tttgtcaaac tgcccacgga cttttttgcc acaatggctg
     2101 caaacacctt cgtacacacg ttcctctgta acaatggttc gaatttcaat atctatcttt
     2161 tgcttagcct cgaaagcttg attatttatg atactatgtc cgcattcgtc acagacttca
     2221 accttttttt caataaccgt gtctggatct gcaaatagct tcaatccatg ccctggatgc
     2281 cccaattgtc ctccgggctt cttaccactt ttttcacgta ggttttgtgg tttcggtttg
     2341 tggaaaacat ctgtcgatgg cggcttactt gacgatgtgc tgtccttttt tagcctggca
     2401 agaagcttct tgatatgcat tttcattttt tctattgtcg cagtaaacac ggctcgttct
     2461 tttttcagtt cggcattctc atttttcagc tcgtttatct tcatctcgca atcggctatg
     2521 tatttttgta gcatctctat tgcaggggct tgcccggcaa gctcctgctc aagagtatat
     2581 attctgtttt taagggattc ctccattact tctttctttg caaatgcatt gaatagctgc
     2641 gtccgaagat catttaccct tttttcaagc tgctgttttg tcatttgcca tctccattaa
     2701 acgaaaagta atcaccatgt tccatacgat caaaaaattc gcttcttgca taacgctcgc
     2761 ttatgcagcg attagggcat acctcacagg atgataacag ctcatcccat gcctctccgg
     2821 caatgtccat gataattaga ttctgtttgt gcgtgttctc atgacagtag tccgcaatat
     2881 attcttcatc tgcggtagta gcatctgtga gcccatttag tttgtcgaga acagcctgtt
     2941 tcattcgttt tgcaaaatac gcacagaatt ctgcaacttg aagatcactg cgctcagatt
     3001 tccacttttt aagccgctcg cttttcctga tatggtcata aagcatacag gcatattcgt
     3061 aacagaacca gttgaatttt atcttctcac tcgcaaacat atagaaccat tttgctttga
     3121 taggtggatc gattttcata gggcaccccc tgaacggaaa aatctgtttg tatcaatgtt
     3181 gactgttcct tatctgttct attatctctt ctgttatatc aggtctaacc cgacatccca
     3241 tacgaatttc atttcacatg aagttcataa tgtaatattc gacatcg
//
LOCUS       DAOSDD010000376         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10432_length_3251_cov_5.433041, whole genome shotgun sequence.
ACCESSION   DAOSDD010000376 DAOSDD010000000
VERSION     DAOSDD010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_3251_cov_5.433041"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PK733_19285"
     CDS             complement(<1..880)
                     /locus_tag="PK733_19285"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12714.1"
                     /translation="MHKKMVILGAGSAMFTQGLVMDMLDKNPGGHKWHLALVDIDEHV
                     LNGVAKLVKKMVEKKKADIELSWSTDRKDVLQGADYVVSTIGVGGRRAWEQDVFIPRK
                     YGINQPVGDTAMPGGISRAMRMVPATLEIVRDIQKFCPKAYFFNYSNPMAIICRAVRK
                     ALNYPITGLCIGTVGSERHIADFMGYERSRVTSLAAGINHCTFIYDFRYNGEDVWPAV
                     RKKLSKMLEVYGETEACNDDIGSHIIEKSKSNESIDKTFHMGEPFAWKFFLRYGAFPA
                     PGDRHITEFFTEYFPGG"
     gene            complement(1157..2050)
                     /locus_tag="PK733_19290"
     CDS             complement(1157..2050)
                     /locus_tag="PK733_19290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12715.1"
                     /translation="MKALAVYRLLDLEQGHEDLSEYLLVNCAGYYEAEESFGATHRLY
                     GRKDWYLSFNRAGRMKYMNKKLMFDVQDNMVFIYAPNEEQYYGQADDNPIENYWVHFT
                     GSGAALILEKLGLTSGVYSVNTDLSSYYEKMIDELANKLYGFEAASSAWLVMILTEVA
                     RYTHKKIGPVSTADRRYNEIAKTVQYIKDNYHRKITVTELAELSHFSISYYCSIFHEI
                     LKKSPQKYLLEYRLYRASDLVIRTRLNICQIAQMTGFNDQLYFSRKYKQFYGCSPSEH
                     RKKHQDTDKEASYNPSIQGYV"
     gene            complement(2225..3160)
                     /locus_tag="PK733_19295"
     CDS             complement(2225..3160)
                     /locus_tag="PK733_19295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ12716.1"
                     /translation="MDKQSIEKQYDIITALDICVDLIVKGNVEPQFNQAEKLVDDYYL
                     EMGGTACIFACQCARLGLTTAGIGVAGRDLFGELAIQRLKKCGVDITAVRIYDFLKTG
                     LGIALCKGNDRAILTYTGTIDAVKPEWIEEKLHLARHLHIAGYYLMSSLKMHWERLIS
                     YAKSCGLTISLDTNWDPSELWDGIYKLSQYIDLFMPNDNEVMAITGEKDALRGLEKLS
                     GYFKTVVMKMGEKGAAAISDGKFQYTESFPVKVVDTIGAGDSFNAGFLYGWLNSLPMK
                     DCLSIGCFCGSSNVAMQGGIEGQPCLKDLAHNYLR"
BASE COUNT          964 a          711 c          493 g         1083 t
ORIGIN      
        1 tgcctccggg aaagtattcc gtgaaaaatt cggtaatatg cctgtctcca ggtgccggga
       61 atgctccata tctcaaaaag aacttccatg cgaagggttc acccatatga aaggtcttgt
      121 caatcgactc attacttttt gatttttcta tgatatggct gccaatatca tcattgcaag
      181 cttctgtttc tccatacact tccagcattt ttgaaagctt ttttcgtact gccggccata
      241 cgtcttcacc gttataccgg aaatcatata tgaatgtaca atggtttata cctgctgcaa
      301 gtgatgttac tctcgaacgt tcatatccca taaaatctgc gatatgcctc tctgagccaa
      361 ctgtacctat acaaaggcct gttataggat agttcaatgc ttttctcaca gccctgcata
      421 taatagccat aggattgctg taattgaaaa agtaggcttt gggacaaaat ttctgaatat
      481 cccggactat ttccagtgtt gcaggaacca tcctcatagc tctggatatg ccaccaggca
      541 tagctgtatc acccacaggc tggttaatac catattttct aggtatgaat acatcctgtt
      601 cccatgccct tcgcccgcct acaccaattg ttgatactac ataatcagca ccctgtaata
      661 catccttccg gtctgttgac catgaaagct caatatcagc tttcttcttt tccaccatct
      721 tctttacaag ctttgcaact ccgtttaaca catgctcgtc aatgtcaacc aaagccaggt
      781 gccatttatg tccaccagga tttttatcaa gcatatccat tacaagacct tgtgtaaaca
      841 ttgcactgcc tgctccaagg ataaccatct tcttatgcat attccttcat ccttcctata
      901 aattgtattt atatataacg caaaaatgat tctacaagga acatattgaa tttttgcgct
      961 ttcattaatg taacataaac gcacaaaaat tttatatgca ttttcttttg tgcgttatct
     1021 atagtcacaa tcaaaaaata aattatctaa tcgggtttta aaaaaagtgg tattcaaaat
     1081 cattgtaagc aatccttcca tatcagaatt tttaataccc aggtagtagg tagtaattaa
     1141 cgttaatcct attatcttac acataaccct gtatacttgg gttatatgat gcttctttat
     1201 cggtatcttg atgctttttc ctgtgttcag aaggtgaaca cccataaaac tgcttatatt
     1261 tcctgctaaa atatagttga tcattaaaac ctgtcatctg cgcaatttgg catatattca
     1321 atcttgtacg tatcacgaga tctgaagccc tataaagcct gtattcaagc aaatattttt
     1381 ggggagattt tttaaggatt tcatgaaata tgctgcaata atagctgatg ctgaaatgtg
     1441 ataactcagc cagttcagta actgtaattt ttctatggta attatcctta atatattgaa
     1501 cagttttggc aatttcattg tatctcctat ctgcggtaga tactggacct atctttttat
     1561 gtgtataacg tgctacctca gtaaggatca taacaagcca ggccgaagat gctgcttcaa
     1621 atccgtaaag tttatttgcc agttcatcta tcattttttc ataataactg gaaaggtctg
     1681 tattaacaga ataaactccg gaagtcagcc caagtttctc taaaattaag gctgcaccag
     1741 accctgtaaa gtgaacccaa taattttcta ttgggttatc atcagcctgt ccataatact
     1801 gttcttcatt tggcgcatat ataaatacca tgttatcttg tacatcaaac atcagctttt
     1861 tgttcatata tttcatacgt ccggcgcggt taaaagacaa gtaccagtct tttcttccat
     1921 aaagcctgtg agtagcacca aaagattcct cagcttcata atatcctgca caattaacca
     1981 gcaaatattc agaaagatct tcatgtccct gttccaaatc aagaagtcta tatactgcaa
     2041 gggctttcat tgctttttca ccccttatat atatatttca aatgcatatt attatagatt
     2101 attttatatt atacaatcca ttttgtatac taagttttgg caaattcatg tttttatctc
     2161 agcatacagc atctttgcat atctttatct ttaataatat gcaatacatc ctgtccataa
     2221 tattttacct cagatagtta tgagcaagat ctttaaggca aggctgtcct tcaatgccac
     2281 cctgcattgc aacattgctg cttccacaga aacagcctat actaaggcaa tctttcatag
     2341 gtaaactatt cagccatcca tacaggaagc ctgcattaaa tgaatcacct gctcctatag
     2401 tatccactac ctttaccgga aatgattcag tgtattgaaa tttaccatcg gatattgcag
     2461 cagcaccttt ttcgcccatc ttcatgacaa cagttttaaa atatcccgac aatttttcca
     2521 gaccccttaa tgcgtccttt tcacctgtaa tagccattac ctcattatca tttggcatga
     2581 atagatcaat atactgtgat agtttatata taccatccca aagctctgaa ggatcccagt
     2641 ttgtatccag acttatggta agcccgcagc tttttgcata agaaattaat ctttcccagt
     2701 gcatctttag agaggacata agataataac cggcaatatg caaatgtctt gcaagatgaa
     2761 gcttttcttc tatccattca ggtttgactg catcaatagt accggtatat gtaaggatag
     2821 cgcggtcatt acctttacat aaagctatac ccagtccggt tttcaagaaa tcataaattc
     2881 taacagcggt tatatctact ccacattttt taagccgttg tattgctaat tctccaaaca
     2941 aatcccggcc tgcgactcct atacctgcag ttgtaagtcc taatctggca cattggcagg
     3001 caaaaatgca agcagtccct cccatttcca gataataatc atctacaagt ttttcagcct
     3061 ggttaaactg aggctcaaca ttgcctttta ctataaggtc aacacatata tcaagtgctg
     3121 ttataatatc atattgtttt tctatagatt gcttatccat aaaaatcctc tctaatatag
     3181 tgcctggtac ggcacttata tagaacaggt tttaaaacat ttaaaacctg cttctgttac
     3241 cttgatgtaa c
//
LOCUS       DAOSDD010000377         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10454_length_3245_cov_3.626332, whole genome shotgun sequence.
ACCESSION   DAOSDD010000377 DAOSDD010000000
VERSION     DAOSDD010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10454_length_3245_cov_3.626332"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PK733_19300"
     CDS             complement(<1..1305)
                     /locus_tag="PK733_19300"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12717.1"
                     /translation="MINNPQIGLLPLYIELYDISNPEMRERVNAFLHTIETELKKRDL
                     QVITAPVCRLRPEFENAVKSFEAQDVDVIVTLHLAYSPSLESADVLAKTKLPVVVLDT
                     TPTYEFGPEQNPVEIMYNHGIHGVQDMCNLLIRNKKHFMLEAGHWEKSDVLDRVAGCV
                     KAAKLAENIRSARVGIVGESFKSMGDFAVPTEVLKNAIGIETISFDFYKAQESVDQVK
                     DEDILMEMEENKSLFEVNGLDDKVHKHSSYVNLLIRNWIENEKLTAFTVNFLAISGKS
                     IISTMPFLEASKAMGRGLGYAGEGDVLTAALVGALASVYPETSFTEMFCPDWKNNSIF
                     LSHMGEMNINLTAEKPVLIEKEFPYADAYNPIVAYGRFKAGEVIFVNLAPKADNKYSL
                     ILSKGNMLGVSGTDNMKNSIHGWFEPTISIDKFLTEYSLSGGT"
     gene            complement(1568..2617)
                     /locus_tag="PK733_19305"
     CDS             complement(1568..2617)
                     /locus_tag="PK733_19305"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ12718.1"
                     /translation="MAQLFEVKEIDVKFYEEQLKEFLPDQIIDIHTHVWLNKFRVKEE
                     DACNMPVRSATWPSLVAKDNSVEDLIQTYKLMFPDKKVTPLIFGHPLIEYDIAKSNDY
                     IEECSKRFGFPSLILSIPEWSGAELEEKIIRGGFLGSKVYLNYAKPYIPQNEIRIFDF
                     LPEHQLDVLNKHGWIVMLHLPRNKRLKDPVNLAQLLEIEQNYPRLKLVVAHVGRTYCP
                     EDVGDAFEVLTGTKNMVFDFSANTNQFVFERLIETVGASRILFGSDMPILRMRMRRIC
                     ENGNYVNLVPKGLYGNISGDVHMREVDGADAEKLTYFMYEEIAAFKHAAASMELKKED
                     IENIFYNNAVSILGL"
     gene            complement(2916..>3245)
                     /locus_tag="PK733_19310"
     CDS             complement(2916..>3245)
                     /locus_tag="PK733_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ12719.1"
                     /translation="IEEFRERGCKLDHLRMIGGGSKSNLWTDIIANVTGAPVIRLKES
                     EAACIGAAIIAGVGCGVFKDYSDACEHLVKDEIVTAIDTVKRDFYKAKYEKYKQGIKS
                     IESYYQN"
BASE COUNT          942 a          691 c          459 g         1153 t
ORIGIN      
        1 tgtgccacct gacaagctat attccgttaa gaacttgtca atggagatgg ttggctcaaa
       61 ccatccatgt atactgtttt tcatgttatc tgtaccgcta acaccgagca tattgccttt
      121 tgacaaaatc aagctgtatt tgttatcggc ttttggtgca aggttaacaa atattacttc
      181 tccggcttta aaccttccgt aagcgacaat aggattatat gcatccgcat atggaaattc
      241 tttttcaata agtacaggtt tttctgctgt aagattaata ttcatctcgc ccatgtggct
      301 caagaagatg ctgttatttt tccagtcagg gcaaaacatt tctgtaaatg atgtctccgg
      361 atatactgat gctaatgcac ctactaaggc tgcagttaat acatcacctt ctccggcata
      421 acctaatccc ctccccattg ctttgctggc ttccaaaaaa ggcattgttg aaattattga
      481 tttgcctgat attgcaagaa aattaacagt aaaagctgta agtttttcat tttcaatcca
      541 attccttatt aataggttta cataggatga gtgtttatga actttatcat ccaaaccatt
      601 aacctcaaat aaagatttgt tttcttccat ttccattagt atatcttcat ccttaacttg
      661 atcaactgac tcttgagcct tataaaaatc aaaactaatt gtctctatac ctatagcgtt
      721 tttaagtact tcagttggaa cggcaaaatc tcccatgctt ttaaaagatt cacctactat
      781 cccaacacgg gcactgcgaa tgttttctgc aagtttagcc gcttttacac aacctgcaac
      841 tctgtctagt acatcgctct tttcccaatg tcccgcttcc agcataaaat gttttttatt
      901 gcgaataagt aggttgcaca tatcctgaac tccatgaatg ccatgattgt acataatttc
      961 aaccggattc tgctccggac caaattcata tgttggagta gtatccagaa caactacagg
     1021 caactttgtt tttgcaagga catctgcaga ttctaaagat ggggagtaag caagatgaag
     1081 ggttacaata acatcaacat cctgcgcctc gaagctttta acagcatttt caaattccgg
     1141 tctaagtctg cacacaggtg ctgttataac ttgtaagtct ctctttttca actctgtttc
     1201 aattgtatgc aaaaacgcat ttactctttc tctcatttca ggattagata tgtcatataa
     1261 ctcgatatat aaaggcaaaa ggccaatttg ggggttattt atcatataca ttttcctttc
     1321 tttattaata ttttatttag ctacacaaca caaaatacaa gggggcagtt ctcgcgtaca
     1381 cataacactt ttgagaacaa ttctcgtgtg tcgtttcatg ttttggtaaa aaataatgcc
     1441 ccgagtccgg ttttttttgc cggcacctgc tctatatcca tcttattttt actcctgact
     1501 agttaacagt attctttata cctttatacc tttatactgt tgtaattcca aatgctggta
     1561 gaataactca taaacctaat atacttacag cattattata aaaaatattt tctatatctt
     1621 ccttttttag ttccatggaa gcagcagcat gcttaaatgc ggcaatctcc tcatacataa
     1681 aataagttag cttttccgca tcagccccat caacttcacg catgtgaaca tccccggata
     1741 tattcccgta taagccttta ggtacaagat ttacataatt tccattttca catattcgcc
     1801 tcattctcat gcgaagtatc ggcatatcac tgccaaacag tattctactt gctccaactg
     1861 tttctattaa tctctcaaat acaaattgat ttgtatttgc actaaaatcg aataccatat
     1921 tttttgtgcc ggtaagtact tcaaatgcat ctccgacatc ttctgggcag taggttcttc
     1981 ctacatgagc tactaccagt tttaaccttg ggtagttttg ttcaatttcg agaagctgtg
     2041 ccagattcac aggatccttt aatcttttat tcctcggcaa atgaagcatt acaatccatc
     2101 catgcttatt taatacatca agttgatgtt cagggagaaa atcaaaaatc cttatttcat
     2161 tttgaggtat gtacggtttt gcataattaa ggtacacttt acttccaaga aaaccccctc
     2221 ttataatctt ctcttcaagt tctgctccgc tccattccgg tatagacaag atcaacgaag
     2281 gaaatccgaa cctttttgaa cactcttcta tataatcatt agattttgca atatcatatt
     2341 caatgagtgg atgtccaaaa attaatggag taactttttt gtctggaaac attagtttat
     2401 aagtttggat aagatcttca acagaattat ctttagctac aagtgaaggc catgttgctg
     2461 acctgacagg catattacat gcatcttcct cctttactct gaatttatta agccatacat
     2521 gggtgtgtat gtcaataatt tgatcaggga gaaattcttt taattgctcc tcgtaaaatt
     2581 taacatcgat ttcttttact tcaaaaagtt gtgccactga aaccactcct cccttcctat
     2641 acattgtcta tccttaagct gtctagtctt acattattta gtcttatatc atttagtcct
     2701 gtgttatcat agtcttatgt tattatagtc caatatcgaa ttaacttgta ttgttatatg
     2761 ctttgtatct ttttagcttg attcttaaaa tcttttcagc tgaataattt ttcaaatatt
     2821 tttttactca aatactcaca tattgctatt aaaacattac atcaagactt cgtacaatac
     2881 cttgtcttta ggtatatcat tctcaagcaa tagcatcaat tttgataata gctctcaata
     2941 gactttatac cttgcttata tttttcatac tttgctttat aaaaatctct ttttactgta
     3001 tcaatagctg taactatctc atccttaact aaatgctcac aagcatcact ataatcttta
     3061 aaaactccac atcctacccc tgctattatt gccgcgccaa tgcatgcagc ttccgattct
     3121 tttaacctaa ttacaggagc tcctgtaaca ttggctataa tatcagtcca aagattgctc
     3181 ttacttccac caccaatcat tcttagatga tccagtttac accctctttc cctaaattct
     3241 tcaat
//
LOCUS       DAOSDD010000378         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_10745_length_3182_cov_4.804285, whole genome shotgun sequence.
ACCESSION   DAOSDD010000378 DAOSDD010000000
VERSION     DAOSDD010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10745_length_3182_cov_4.804285"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PK733_19315"
     CDS             complement(<1..378)
                     /locus_tag="PK733_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ12720.1"
                     /translation="MLNFQNIKLNNRIPVYVQIALYVKQQILLGKVVSGDELPSRREI
                     AAQLNINPNTAQKAFKLMEEEGYVLTNGNQGSIIYIDESVLSKIKTELTNEMVNEFIS
                     SAREINLSFKDVIDLISKFWDGGN"
     gene            773..1609
                     /locus_tag="PK733_19320"
     CDS             773..1609
                     /locus_tag="PK733_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOJ12721.1"
                     /translation="MYDFKVGDIVARKSYGGDIYFRIADIIEKSGGKQTYILKGLFYR
                     IEADSNGDDLVKQDTGVAYRSIEKDIYTARNNMYRNASTMNRARIFPFLRLSTEPGRI
                     LHIDADESFLNMCLRHYKEYRIPAVGKLAKESEQPGVVRKYLETYRPNVLVLTGHDSF
                     KKNSDKNNIGSYSNSKYFIESARIARKYESNPDRLCIFSGACQSYYEQIMDAGANFAS
                     SPGRILINALDPAFVAQKISLTDSRTAVTPDKAVALTISGSKGIWGINTKGQLKARYA
                     FM"
     gene            1970..>3182
                     /locus_tag="PK733_19325"
     CDS             1970..>3182
                     /locus_tag="PK733_19325"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HOJ12722.1"
                     /translation="MGRILKIQQVLKVRQIFKTFMKGKLRLLFAAVPLIALISIFGVS
                     ATKAWEVNIDNKYICVAKTKDVVQGIMDDIKTEAENRYGTEIVIPNKISFNSVLFANK
                     KIVNEKEIRNQLEGYATLIAKAFAINVDGKPIALLKDKTSAEEVLNRIKEPYAEDGEG
                     DVHIGFVEDVVIAEKEVPVNELKEPDDVFKSIFLQNDQVKKYTVEKGDTISEIAEEFG
                     MKVADIKKANPEINVDKISIGQEICLMVPRYTINVKKMTYTTYEENIPFEQKSENSGD
                     LYRGESKVKVKGIEGRKLVKAEIVSINGIVEGTNIIDEEVLETPKDEIVFRGTKERPR
                     TLAYGEFIMPSRGSISSRFGQRWSSQHTGIDIAVPRGTPNKAADGGVVTFAGWSGGYG
                     KLVIIDHENGYT"
BASE COUNT         1140 a          493 c          632 g          917 t
ORIGIN      
        1 attgcctcca tcccaaaatt tacttattaa atcaattaca tccttaaagg agagatttat
       61 ttctctggca gaacttataa attcatttac catttcatta gtgagttcag tttttatttt
      121 tgacaaaaca ctttcatcta tataaataat actgccctga ttgccatttg taagaacata
      181 accttcctcc tccattaact taaaagcttt ttgagcggta ttcggattaa tatttaattg
      241 tgccgcaatt tctcttcttg atggcaattc atcacctgac acaacttttc ccagtaatat
      301 ttgttgtttc acatacaaag ctatttgtac atatacaggg attctgttat ttagcttaat
      361 attttggaaa ttcagcacaa gttactcacc atccctattc catcccattt tatcctattt
      421 cacttcatac aagccactac catttatgat agttgacagt tgaccctatt ctactggtta
      481 cactcggtga tatacaaggt taatacataa ccggagtttt gtgtctacaa gtatgcgcga
      541 tataccttat tatgtaaggt taatatgcta ttaaccggta aatttactct ctatgtgtat
      601 tatacgacta atacacatgc aaagtcaatg ggattcttta tatgcgcctt atattatacg
      661 attatacgat taaccttaaa aagttgacag aaaaataaag tttcctattt accacccaat
      721 taaggtcatt tgcatattat gaagtaatta ataatcttat ggagtggtaa ttatgtatga
      781 ttttaaagtg ggggacatag tagcaagaaa atcttacggc ggagatattt attttagaat
      841 agcagatatt attgaaaaaa gtggcggtaa acaaacctat attttaaagg gacttttcta
      901 cagaatcgaa gcggattcca atggggatga tcttgtaaaa caggataccg gtgttgctta
      961 tcgaagtata gaaaaggata tttatactgc tagaaacaat atgtatagaa atgcatcaac
     1021 aatgaacagg gcacggattt ttccttttct tagactaagc accgaacctg ggaggatact
     1081 tcatattgac gctgatgaga gttttttgaa tatgtgcctt aggcactaca aagaatatag
     1141 aatacctgca gttggcaaac tggcaaaaga gagtgaacaa ccgggggtag taagaaaata
     1201 cttggaaaca taccggccaa atgtactagt gctgacaggg catgatagtt tcaaaaaaaa
     1261 ttccgataaa aacaatattg gaagctatag caattcaaag tactttatcg aatcagccag
     1321 gatcgcaaga aagtatgagt ctaatccaga cagactttgc atattttccg gagcgtgtca
     1381 atcttattat gaacagataa tggatgcagg ggcgaacttt gcaagctctc ctggcagaat
     1441 actgattaac gccctggacc ctgcgtttgt agctcaaaaa atatcgctta cagactccag
     1501 aacagcagta acacctgaca aagctgttgc tttaacaatt tcaggtagta agggaatatg
     1561 gggtattaat acaaaaggac aacttaaagc aaggtacgcg tttatgtaaa aataatggga
     1621 taaaaataat ggggacactt ctctactttt ctaaaattaa cagataatga aaacgaaggg
     1681 gacacttatc ctgaaataat ggggacactc ctctacttct caaaataagt gaataaggaa
     1741 ggaaagagaa taagggggga cagatctgga gaactgtccc ccaacacaca ccccaacaca
     1801 caataaacca tttacatatg caaatggtat ggtataataa aaaatataca agtttccgtg
     1861 cttgtatatt gatattataa cgaggcaata gatgtcaccc gcctctatag gtggcattgc
     1921 actgatagaa taatgattaa ttaaataatg attaatcggg agataaataa tgggacgaat
     1981 attaaagata cagcaggtat taaaagtacg gcagatattt aaaacgttta tgaaaggtaa
     2041 attaaggctt ctttttgcgg cagtaccgct aattgcttta atttctatat ttggtgtttc
     2101 tgcaacgaaa gcttgggaag ttaatattga taataaatac atatgtgtag ctaaaacaaa
     2161 ggatgttgtg caaggaataa tggatgatat taaaactgaa gcagaaaatc gctatggaac
     2221 tgagattgtt attcctaata aaataagttt caatagtgtg cttttcgcta acaaaaaaat
     2281 agtcaatgaa aaagaaatca ggaaccaatt ggagggatat gcaacattaa ttgctaaggc
     2341 ctttgcaatt aatgtagatg gtaaaccaat tgcattacta aaggataaaa cttctgcaga
     2401 agaagttcta aacagaatca aagaacctta tgccgaggat ggagaaggtg atgtacatat
     2461 tggttttgtt gaggatgttg taattgcgga aaaggaagta cctgtaaatg agctgaaaga
     2521 accggatgat gtttttaaaa gcatatttct tcagaacgat caggtaaaaa aatatacagt
     2581 agaaaaggga gatacaatat cagagattgc agaagaattt ggaatgaaag tagcagatat
     2641 taagaaagca aatcctgaga taaatgttga taaaatatct attggacaag agatatgttt
     2701 aatggtgccc aggtacacta taaatgttaa gaaaatgaca tatacaactt atgaagaaaa
     2761 tatccccttt gagcaaaaat ccgaaaacag tggagattta tacagaggtg aaagcaaggt
     2821 caaggttaag gggattgaag gacgaaaact ggtaaaggca gagattgtta gtattaatgg
     2881 tatagtagaa ggaactaata ttattgacga agaagtttta gagactccga aagatgaaat
     2941 tgtgttcaga ggaactaaag agcgaccacg gacccttgca tatggtgagt ttattatgcc
     3001 cagcagaggc agtatttcat cacgttttgg tcaaagatgg agctcacagc atacaggaat
     3061 agatatagct gtaccgagag gtactcccaa taaagcagcg gacggcggag ttgtaacatt
     3121 tgccggatgg agtggaggct atgggaaatt agtaattatt gaccatgaaa atggttatac
     3181 ca
//
LOCUS       DAOSDD010000379         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11017_length_3126_cov_3.465972, whole genome shotgun sequence.
ACCESSION   DAOSDD010000379 DAOSDD010000000
VERSION     DAOSDD010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11017_length_3126_cov_3.465972"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..2712)
                     /gene="glgP"
                     /locus_tag="PK733_19330"
     CDS             complement(145..2712)
                     /gene="glgP"
                     /locus_tag="PK733_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ12723.1"
                     /translation="MFLTGKIIVNPILPERLSRLKDIAYNLWWSWNFDALDLFEDIDS
                     DLWEGTGKNPIFFLQEVSSNKLEEALNNKAYMLKFGEVVDEFDSYLSDKNTWFNRNYP
                     NIGNHVVAYFSAEYGLNEILPIYSGGLGVLSGDHCKSASDLGIPFTAIGLLYKQGYFS
                     QRINKDGWQETVFSDLDVSRLPLQPALNNEGEQVLINIELPGRMVYAKVWEVKIGRVS
                     LYLMDTDIEQNNESDRWLTARLYGGDQETRVQQEILLGIGGVRILEALCINAWVYHMN
                     EGHSAFLGLEIIRKHIHEKKLSFKAAKEVAQAHIIFTTHTSVPAGNDVFPLYMIDKYF
                     SNYWNNLGISRHQFLDMGLKVNDNQNFNMTVLALTLAGRKNGVSELHGAVTRNIYNNL
                     WPGVPEDEVPIIHITNGIHTTSWLSASLEKLFDKYIGKEWQEKISEKSIWQNIDKVPD
                     NQLWSIHIQLKNELIVYLREKIKLQRISNGCSPENIRETDHLLDPDALTIGFARRFAT
                     YKRANLIFRNIERIKSIFNNPEMPVQIIFAGKAHPADRPAHEVIKYINDIASSEGFTG
                     KVVLVEDYNIALARKMVQGVDIWLNNPRRPLEASGTSGQKVCINAIINFSILDGWWCE
                     GFNGNNGWVIGNGTYCDDEYYQDNADSESLYSILESQIIPLYFRRDANGIPTGWTRLM
                     KESIKSLAYRFSTHRTVQDYTNKMYIPSITRVDKIISSYYSYAINLAEWKSFIEENWS
                     QVSISAEKTLNHLKEYKTPAGNSIAISANVNMGNIDPLNVKVEVYYRKLNHSNDIEEP
                     QVKEMKMIEKTGSASWLYTAEIKLDDGGEYSYTFRVIPYHPDLINKFDTGLIRWVVQ"
BASE COUNT          920 a          615 c          472 g         1119 t
ORIGIN      
        1 ccaattaaaa ggctctacag caccttcact tatgcccctt accaatattt tcttcactta
       61 aattaaatgc acccttggtg ttaatctaaa agaggttatc tcttactttc caccttttac
      121 ttcctacctc tcacctctca tttcttattg caccacccat cttataagtc ctgtatcaaa
      181 tttattaatt aaatcaggat gatagggaat tacacggaaa gtataagaat attcacctcc
      241 gtcatcaagt tttatttcag cagtgtaaag ccaggaagct gaccctgttt tttcaatcat
      301 tttcatttct tttacctggg gctcttcaat gtcattactg tgatttagtt ttctataata
      361 aacttctact tttacattta atgggtctat attacccata tttacattcg cagaaatagc
      421 aatcgaattg ccggccggtg tcttatattc tttaagatga ttcaatgttt tttctgcaga
      481 tatggatact tgtgaccagt tttcttcgat gaatgatttc cattctgcca ggttaattgc
      541 gtaactgtag taactggata ttattttgtc tactcttgtt atggatggaa tatacatttt
      601 atttgtataa tcctgtaccg tcctatgtgt gctgaagcgg tatgcaaggg attttattga
      661 ttctttcatt aacctggtcc aacctgttgg aatcccgttg gcatctctgc gaaaatatag
      721 aggaataatc tggctttcaa gtatggaata taatgattca ctgtcagcat tgtcctgata
      781 atattcatca tcacagtaag taccgttgcc tatgacccat ccgttgttgc cattaaaccc
      841 ttcacaccac catccgtcca gtatactgaa attaataatg gcattaatgc atactttttg
      901 tccactggta ccacttgctt ccaaaggtct tcgtggatta ttgagccata tgtccactcc
      961 ctggaccatt ttacgtgcca gggcaatatt gtaatcttct acaagaacta cctttcccgt
     1021 aaatccttcg cttgatgcaa tatcattaat atatttaata acttcatgtg ccggtctgtc
     1081 tgccgggtga gctttaccgg caaatataat ttgtacaggc atttcaggat tattaaaaat
     1141 tgatttaatc ctctcaatat tcctaaaaat taaatttgcc cttttatatg ttgcaaatct
     1201 acgagcaaaa ccaatagtaa gcgcatcggg atccaataaa tgatctgttt cccttatatt
     1261 ttcaggggaa cagccattag atattctttg aagtttaatt ttttcccgta aatatacaat
     1321 aagctcattt ttcaattgta tatgaatgct ccaaagctgg ttatcaggaa ctttatctat
     1381 attttgccat atgctttttt ctgatatttt ctcctgccat tctttaccta tatacttatc
     1441 aaaaagtttt tctaatgatg ccgaaagcca ggatgtagta tgaataccat ttgttatatg
     1501 gattatagga acttcatctt caggaactcc aggccataaa ttattgtata tatttctggt
     1561 aacggctcca tggagttcac ttaccccatt ttttctacct gcaagagtta aagctaaaac
     1621 tgtcatatta aagttttggt tatcatttac ttttaaaccc atgtcaagga attgatgcct
     1681 gcttatccca aggttattcc aataattact aaaatacttg tctatcatgt ataacggaaa
     1741 gacatcattc ccagcaggga cagaagtgtg tgtagtaaaa attatatgtg cctgagctac
     1801 ttctttagct gctttgaagg ataatttttt ttcatgtata tgttttctga ttatttcgag
     1861 tcctaaaaaa gcagagtgtc cttcattcat atggtatacc catgcattaa tgcacaaagc
     1921 ttctaaaatt cttaccccgc ctatgccaag cagtatctcc tgttgtactc tggtttcctg
     1981 atcaccacca tataaacgtg cagtcagcca tctatcagac tcattatttt gctctatatc
     2041 agtatccatc agataaagtg atactcgtcc aatttttact tcccacactt ttgcataaac
     2101 cattctgcca ggcaattcaa tattgatcag tacttgttct ccctcattat ttaaggcggg
     2161 ttgcagaggt agcctggaca catccaaatc ggagaaaact gtctcttgcc atccgtcctt
     2221 attaatccgt tggctgaaat aaccttgctt gtatagtaaa cctattgccg taaaggggat
     2281 accaagatca ctggcagatt tacagtggtc accagataaa acaccaagtc cccccgagta
     2341 tataggcaat atctcgttta agccatactc agccgaaaaa tatgctacca catgattacc
     2401 tatattcgga taatttctat taaaccaggt atttttatct gataaatatg agtcaaactc
     2461 atcaactact tcaccaaatt ttaacatata tgccttatta ttcaaggctt cttcgagttt
     2521 attactactt acttcctgaa gaaagaaaat agggttttta cctgttccct cccaaaggtc
     2581 ggagtcaata tcttcaaaca agtctaatgc atcaaaattc caggaccacc aaaggttata
     2641 tgcaatatct ttcaaccttg acagcctttc tggaagaatg gggtttacaa ttatttttcc
     2701 agttaaaaac atacgcatcc tccaatttat aaattgtatt ttaatttcaa atttcattat
     2761 tattgttatt atctatttat tagtttatta ttagcataat ttcattttat aatattatga
     2821 tttataatat aattattact gttgttttgc atttttatta taaaacctga ttttaagcta
     2881 tttgtatctc agtatgaaag tatatgatat caagactttt agctaaaagt gcaaaactat
     2941 agaattatta taataaaaca acactggtta tttgttatag tgttgagcat attgctattt
     3001 taccttaaaa tattatgtag ctatacggtt actatgagca agtatatgcg gttcaggagg
     3061 gtgcgttttt taaaaagtga agaaatattt accaggagct gacgttacgc cgcttagagc
     3121 ctttaa
//
LOCUS       DAOSDD010000380         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11128_length_3103_cov_4.779856, whole genome shotgun sequence.
ACCESSION   DAOSDD010000380 DAOSDD010000000
VERSION     DAOSDD010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11128_length_3103_cov_4.779856"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK733_19335"
     CDS             complement(<1..526)
                     /locus_tag="PK733_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOJ12724.1"
                     /translation="MASLLKGIEVTKAMKEKMLLDVDNLNKKGILPTLGIIRVGARPD
                     DLAYERGAIKRCEGIGVGVRVFEFPEDISQEEFASQVKKINDDEKIHGILMFRPLPKH
                     IDEENVKFLISPEKDIDCMNPVNIAKIFAGDESGFAPCTPEAVIEMLDYYDISIQGKN
                     IVVIGRSMVVGKPLS"
     gene            complement(669..1301)
                     /locus_tag="PK733_19340"
     CDS             complement(669..1301)
                     /locus_tag="PK733_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOJ12725.1"
                     /translation="MAHKSCKEFVNILASKEPVPGGGGASALVGSIGMALGSMVGNLT
                     LGKKKYENYQDDIKNILEKAKNLQQELLELVEKDAEVFEPLSKAYGLPKNTEEEKGKR
                     DAIMEEALKIACSVPLEIMRTSLKAIELHEELAVKGTKLAISDVGVGVLFCKAALMGA
                     SLNVFINTKLMKDREYAVKINNEAKNMLEEGISKADKIYISIEAEMLPDI"
     gene            complement(1568..>3103)
                     /locus_tag="PK733_19345"
     CDS             complement(1568..>3103)
                     /locus_tag="PK733_19345"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ12726.1"
                     /translation="SIKDKENGKLILVTAITPTQAGEGKTTTTVGLGDALSRLGRKAI
                     IALREPSLGPVFGIKGGAAGGGYAQVIPMEDINLHFTGDIHAIGAANNLLAAIIDNHI
                     YQGNELGLDTRKITWKRCMDMNDRQLRFIVNGLNGRTNGIPREDGFDITVASEIMAIL
                     CLARDIEHLKDKLRNIVVGYTRDDVPVTAGQLKAEGAMAALLKDALKPNLVQTLEHTP
                     AFIHGGPFANIAHGCNSIMATQMALKLTDYVVTEAGFGADLGAEKFLNIKCRTAGFKP
                     SAVVIVATVRALKLHGGASKNELGIENLGYLEKGMPNLLKHIENITVKFGLPAVVAVN
                     RFPTDTEVELKFVEDKCREYGVNVALSEVWAKGGEGGIELAKEVVRLIDEGKTNFKYL
                     YKEDDSIKDKIETIVKEIYGGDGVDFAPSALKEIERLEANGYGKLPVCMAKTQYSLSD
                     DQKKPGKPIGFRVTVRSAKVSAGAGFVVALTGDIMTMPGLPKVPAAEKIDVDSTGRIS
                     GLF"
BASE COUNT          820 a          731 c          468 g         1084 t
ORIGIN      
        1 tggacagagg cttccctaca accatgcttc ttccaattac tacaatgttt ttaccctgta
       61 tcgatatgtc atagtaatcc agcatttcaa ttactgcctc aggtgtacat ggcgcaaatc
      121 cgctttcatc ccctgcaaaa atttttgcaa tattaacagg gttcatacaa tctatatcct
      181 tctcaggtga tatgagaaac ttaacatttt cttcatctat gtgcttcggt aaaggcctga
      241 acataagtat gccgtgaatt ttttcatcat cgtttatttt ctttacttgt gaagcaaatt
      301 cttcctgtga tatatcttca ggaaattcaa aaacccttac tccgactcca atgccttcgc
      361 atcttttgat ggcaccacgt tcatatgcaa gatcatcagg tcttgcaccg accctgatta
      421 ttcccagtgt aggtaaaata ccttttttat ttaaattatc tacatcaaga agcatttttt
      481 ctttcattgc ttttgttact tcaataccct tcaacaatga agccatgtaa tttcctcctt
      541 aagtgtcgaa tttagttgac ttgcaccatt tgcatccaat tcatataact ggcaccactt
      601 ttgcttcaat tcaggtttct gggaccattt ctgtttaatt tagatacatg gtagcatttc
      661 tgtttaattt agatatctgg tagcatttct gcttcaatcg atatatatat tttatctgcc
      721 ttgcttattc cttcttccag catattcttt gcttcattat ttattttgac ggcatattcc
      781 ctgtccttca ttaattttgt gttaataaat acattaagac tggcacccat taacgcagcc
      841 ttgcagaaaa gcacacccac tcccacatcg cttattgcca gttttgtacc ttttaccgcc
      901 aattcttcat gaagttctat tgccttcaat gatgtcctca ttatttccag aggaacgctg
      961 caggctatct ttaaagcctc ttccattatg gcatcacgtt ttcccttttc ttcttcagta
     1021 tttttgggaa gcccatatgc ctttgataaa ggctcaaaaa cctctgcatc cttttctacc
     1081 agctcaagta gctcctgttg aagatttttt gctttttcaa gtatattctt tatatcatcc
     1141 tgatagtttt catacttttt ctttcccagt gtaaggtttc ccaccatgct tcccagggcc
     1201 attccgatgg aacctaccag agctgaagca ccgccgcctc cgggaacagg ctcctttgaa
     1261 gcaagtatat ttacaaattc tttgcaagat ttatgtgcta tcatatttta actctccaat
     1321 attaaaaatt tcatataact ataccataaa tttaactata tctataacct tagctataac
     1381 tataattttg actataactg caactgtaat tatttatacc aattcaatcc cagtccatag
     1441 taataagaga ctctttatgt tctatataga acaggtttta aaatgattaa aacctgcttc
     1501 tgttaccttg atgtaactta cgatttgcta attcttcatt gattatttca aatcgtaaat
     1561 tatatagcta aaacaaccct gatatcctgc cggtggaatc tacatcaatt ttttcggcag
     1621 caggtacttt aggcaaaccc ggcattgtca ttatatctcc tgttaatgct actacaaaac
     1681 ctgctcctgc agatactttt gcacttctta cagtaaccct gaatcctata ggcttgccag
     1741 gttttttctg gtcatcggac agagaatact gagtttttgc catacacaca ggaagttttc
     1801 catagccatt cgcttcaagt ctctctattt ctttaagagc tgaaggcgca aaatccactc
     1861 catctccgcc atatatctct ttaactatag tctcgatctt atcttttatt gaatcatctt
     1921 ccttataaag atatttaaag ttggttttgc cttcatctat taacctaact acttcctttg
     1981 ccagttcaat tccaccttca ccgccttttg cccaaacttc agacaaggca acatttacac
     2041 catattctct gcacttatct tcaacaaatt tgagttctac ttcagtatct gtaggaaatc
     2101 tgttcacagc aactactgct ggtaatccaa atttaacagt tatattttct atatgtttaa
     2161 gaaggttggg cattcccttt tcaagataac cgagattttc aatgccaagt tcatttttcg
     2221 aagctccccc atggagttta agtgctctga cagtagcaac tattactact gcagaaggct
     2281 tgaatcccgc tgtacggcac ttaatattaa ggaacttttc agcgccaaga tccgcgccga
     2341 atccggcttc tgtaacaaca tagtcagtta gctttaaagc catctgtgta gccattatgc
     2401 tgttacaccc gtgtgctatg ttagcaaaag gacctccatg tataaaagcc ggtgtatgct
     2461 ccagggtttg aaccagattg ggctttaacg catctttgag gagagctgcc atagcacctt
     2521 ccgcctttag ctgtcctgct gttacaggta catcatccct tgtataacca acaacaatat
     2581 tgcgcagttt atctttcagg tgctcgatat ctcgggcaag gcataaaatg gccattatct
     2641 ccgaagctac agttatatcg aacccgtctt ctctcggaat accgtttgtc cttccattta
     2701 aaccattaac aataaatctt aactgcctgt cattcatatc catgcatctt ttccaggtaa
     2761 tttttcttgt atcaagtccc agttcatttc cctgataaat atggttgtct ataattgctg
     2821 caagtaaatt atttgcggcg cctattgcat gtatatctcc tgtaaagtgc agattaatat
     2881 cttccatagg aataacctgt gcatatcctc cgccagcagc tccgcctttt attccaaaca
     2941 ccggacctaa ggaaggttct ctgagagcaa ttatggcttt cctgcctaac ctgcttaacg
     3001 catctccaag gcccacggtt gtggttgttt taccctcacc tgcctgagta ggtgtaatgg
     3061 cagttaccag aataagcttg ccattttctt tatcctttat act
//
LOCUS       DAOSDD010000381         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11225_length_3083_cov_5.422061, whole genome shotgun sequence.
ACCESSION   DAOSDD010000381 DAOSDD010000000
VERSION     DAOSDD010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11225_length_3083_cov_5.422061"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PK733_19350"
     CDS             complement(<1..931)
                     /locus_tag="PK733_19350"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HOJ12727.1"
                     /translation="MRNREIKKSKLAKNTIITGALCTALIIPAFVQGVDILSASAKDA
                     KVVPIYYKLSHWADTYIEQLSEKYGVESIFKDKNLEAYITLEDFQNIVRLVINEEYSG
                     APDTLTREAVVYELAKMWSEETGQVLDQIPVIKMIIYSDTDKIDMKYNHGITVAYMKN
                     IANGRGGRIFAPKDNVRYGELAALVFNTHKAIDHEQDPKNQPVAQDKFETIGSYEIKD
                     DKVIFDFKLINHYDEPKKLEFSSGQQFEVTITDEKGEEVYKYSDDKFFTMALIYKDIA
                     PAESLKWQDTWNMTSKEGVKLTSGKYRAKINILA"
     gene            1106..2149
                     /locus_tag="PK733_19355"
     CDS             1106..2149
                     /locus_tag="PK733_19355"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12728.1"
                     /translation="MPRRERQKSPQCMYHVMSRSISEIDLYRSDDDKDYYLKLLKRYK
                     DKYHCSIYSYILMDTHVHMYINTCGADISSFMRSLNTAYVKYYNKKYQRHGHLFGGRF
                     ASFIVDNNTYSLTLSAYINNNAKDLPGYRGKEELYAYSSYGIYTGYRDDPDGVVDTDF
                     ILKCFSSDKKEAGKKYRAFTESMSSTGIVKEVDDNITRAYTENEYKSEKSYIVRSEKP
                     DDILGKIEKILGEKLSGGSPKKLSEELPEELQAGLTSGLSTSLSVELQTGLSLGISTE
                     LRLKYSRETSKVRAFVTYTLRTLCGYTYKQLCQYVGNMSLSGISRLVSEGFRLLKEQV
                     VYRNIFNALIQTS"
     gene            complement(2478..>3083)
                     /locus_tag="PK733_19360"
     CDS             complement(2478..>3083)
                     /locus_tag="PK733_19360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ12729.1"
                     /translation="QRKDEIGKLANGVEMLQKSIEEEQALKLELRQVIVKLEDLSIKD
                     PLTGLYNRRYIIEKINELETRYKRSKSLFSIIISDIDYFKRINDRYGHDCGDLVLFNI
                     ARLISDCCREQDILSRWGGEEFLILLSDTNHDGANILAERIRKKLENESIQYGDISLK
                     ITMTFGVSEYSESLGIEGTIKNADMALLQGKHNGRNRVVVL"
BASE COUNT          988 a          570 c          486 g         1039 t
ORIGIN      
        1 tagccaaaat attaatttta gctctgtatt tccccgatgt taattttact ccctctttgc
       61 tggtcatatt ccaggtatcc tgccatttca aagactcggc cggtgcaata tctttgtaaa
      121 taagagccat tgtaaagaat ttgtcatccg aatatttata tacctcttca cctttttcat
      181 cagttattgt tacttcaaac tgttgtccgg agctaaactc gagctttttt ggctcatcat
      241 aatgattaat aagtttaaaa tcaaatataa ctttatcatc ttttatttca taactgccta
      301 ttgtctcaaa tttatcttgt gctacaggct gatttttagg gtcttgctca tggtctatag
      361 ctttatgcgt attaaacacc aaagcagcca gttcaccata tctgacatta tcctttggtg
      421 caaagatcct accacccctg ccattggcta tatttttcat ataagctaca gttatgccat
      481 ggttatattt catatctatt ttatctgtat ctgaataaat gatcattttt ataactggaa
      541 tctgatccaa aacctggcct gtttcttctg accacatttt agcaagttca tatacaacag
      601 cttctctggt taatgtgtct ggtgcaccac tatattcttc atttattaca agtcttacga
      661 tgttttggaa atcctccaat gtaatataag cttcaagatt tttgtcttta aatattgatt
      721 ctacaccata tttttccgat agttgttcta tatacgtatc agcccagtgg ctaagcttat
      781 aataaatagg tacaactttt gcatcttttg ctgacgcaga taatatatct actccctgta
      841 caaatgccgg aattattaaa gctgtacaca aagcacctgt aataattgta tttttagcta
      901 atttactctt cttaatttcc ctatttctca taaataacca tccttttcgc taattttata
      961 ttgtatacta ttagacgatg ataaaagcac attgttccgt tcattgttcg ttcatttgaa
     1021 attaagggga cattgcttca catttatcat ttgttattga cattattttc atattatggt
     1081 aaaatacata ccgaggtgat aaataatgcc aagacgtgaa agacaaaaat ccccacagtg
     1141 catgtaccat gtgatgtcca gaagtattag cgaaatagat ctgtatcgtt ctgatgatga
     1201 taaagattat tatcttaaac tcttaaagcg gtataaagat aaatatcatt gcagtattta
     1261 ctcatatatc ctcatggata ctcatgtcca tatgtacatt aatacttgcg gtgcagatat
     1321 ttcgtctttc atgcgcagtc ttaatactgc ctatgtcaaa tattacaata agaaatacca
     1381 gaggcatggg catttatttg gaggcaggtt tgccagcttc atagttgaca acaatacata
     1441 cagcttgaca ctatctgcat atatcaacaa caatgcaaag gatttgccag gctacagggg
     1501 aaaggaagag ctgtatgctt attcgtcata tggaatatat actggctaca gagatgatcc
     1561 tgatggggtt gtggacactg attttatatt aaagtgcttt agcagcgaca agaaggaagc
     1621 tgggaaaaaa tatagagctt tcactgaatc tatgagtagt acaggtattg tgaaggaagt
     1681 agatgataat atcaccaggg cctataccga aaacgagtat aagagtgaga agagctatat
     1741 tgtaaggagt gaaaagcctg atgatatact gggtaaaatc gagaaaatac ttggtgaaaa
     1801 gctatcaggg ggatcaccga aaaaattatc agaagaatta ccggaagaat tacaagcagg
     1861 attaacgtca ggattatcaa caagcttatc tgtagagtta caaacaggac tatctttagg
     1921 aatatcaaca gagttgaggc taaagtactc tagagaaacc agcaaggtca gggcatttgt
     1981 aacgtacacc ctaaggaccc tctgcggcta cacatataag cagttatgcc agtatgtggg
     2041 aaatatgtcc ttatcaggga tctcaaggct tgtaagtgaa ggtttcaggc tgctgaaaga
     2101 acaggtagtg tacagaaata tctttaatgc attaattcaa acaagctaat actagtctga
     2161 taattaactt gataaatgta ttaattactc tcgtttccta aaacaattaa ataaacaaag
     2221 tgcaataaaa attcgcatta atgataatgg ctaaataaag gagctaaatt aacatgctct
     2281 ttattcttag gccttgcaca aagtgcaaga cctttttgct attgaattat tctttgtctg
     2341 agccatagat ttgaaataaa acactaaaca ttctgtaaat tttcaaaaag tataagaaaa
     2401 atctatataa aactgtatca agtatataaa atatatatat aaatgaaata aaaagagaaa
     2461 tgtccccttt ttccaaatta caatacaaca actctattgc gtccgttatg tttaccctgc
     2521 aataaagcca tatcagcatt cttaattgta ccttctattc caagtgattc actatattcc
     2581 gataccccaa aagtcattgt tattttcaag ctgatgtctc catattgaat actttcgttt
     2641 tctaattttt ttcgaatcct ttctgctaga atgttcgctc catcatgatt tgtatccgac
     2701 aaaagaatca ggaactcttc tcctccccat ctcgatagaa tatcctgctc cctgcaacag
     2761 tcggatataa gtcttgctat attaaaaaga acaagatcgc cacagtcatg cccataacga
     2821 tcattaattc ttttaaaata atctatatcg ctaattatta ttgaaaataa ggatttgctt
     2881 cttttatacc ttgtttctaa ttcattaatc ttttcaataa tatatctccg attatataga
     2941 ccagtcaaag gatcttttat agataaatct tcaagtttca caattacctg ccgcagctcc
     3001 agtttcaagg cttgctcttc ttcaatgctt ttctgcagca tttcaacacc attcgccagc
     3061 tttcctattt cgtcttttct ttg
//
LOCUS       DAOSDD010000382         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11374_length_3047_cov_4.085227, whole genome shotgun sequence.
ACCESSION   DAOSDD010000382 DAOSDD010000000
VERSION     DAOSDD010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11374_length_3047_cov_4.085227"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            715..1845
                     /locus_tag="PK733_19365"
     CDS             715..1845
                     /locus_tag="PK733_19365"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12730.1"
                     /translation="MGNMQIKPTRKKWKGTMTDRERFNNQMHYKEIDRCFNMEFGYWN
                     ENFNEWSIFVENNIINNSQADIFFNFDIIEVVSGNVWINPSFPHKIIEEHEKTNIIMN
                     SDGLMAEVPKDGHDTIPHFIKSSIETPDDWKKVKTERFRRDDPRRKVDVEEIKTAHPE
                     NRDYPLGVNCGSMIGKIRDMLTFEGLAYACYDYPDMVEDMVETCCVLVEDFLDQVLPH
                     IDFDYATGWEDICFKNGPIVSVDFFKNVVTPRYKRISKKLHAVGIDLWYTDCDGDVRP
                     ILPYLLEGGINCLFPFEVNGCSHPAELLSEYGKELRIMGGVDKLQLKKGRKAIKEYLN
                     TLTPLVERGGYIPFCDHRCPPDVSPDDYLYYLDLKEQMFGLK"
     gene            2280..2933
                     /locus_tag="PK733_19370"
     CDS             2280..2933
                     /locus_tag="PK733_19370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ12731.1"
                     /translation="MSRIIIRKYQNPDCKDVLKICYKTGYMGNDLESTGKFDDMKLFG
                     YFFCMYYLLYERDHCFVAVDKDNNCKVVGYIIGTLDTNIQIRLFIKKMLIRIGLRILY
                     TIWKYPDTFKNIMNMLKNRTRKYTPKSLYCDYPAHFHINLLPEYQGMGIGMLLLTEFE
                     VHVKGSAGKGIHLKTSNKNYNAIKFYEKNGYVKLLEFTDILWVGIENYKTVIFTKKL"
BASE COUNT         1076 a          449 c          562 g          960 t
ORIGIN      
        1 tttaaaggct ctaagcggcg taacgtcagc tccgggtaaa tatttcttca ctttaaaaaa
       61 tgcaccccta ttacctatta cctgttacct attacctatt gctttttatt tgctgccaga
      121 ataagtaata catcttttac atgcatatgt tagcttattt aattctatac ataaacacat
      181 atttatagca atccaatatc aacatatgga tgcattatat ccatatggaa tatatttaca
      241 ccagtattag caacattttt atattttgta gcaattatat gtattgatat tattttatac
      301 agattttata ataaagtaaa taggctttcc aatatattta aagtcataag caataatttt
      361 ataaatatgt ttataaccaa ctttataaat tagccttatg agttcaaaat attgatttat
      421 taaaagtaag attaaatatt acgagtttca atgtattgat gatgtattaa cactaaatta
      481 atttttcaat aaaattataa tatggttaac acccgaaaag tcatgaaatg acttttcgat
      541 gaaatttaag gtaccttgag tttcatcagc cttcttaaag aaggcaggat aaccatatta
      601 gattgcacca ggttgcaaca aagctgcaac tccctgcagg ttaacctgca gggaaaagtt
      661 gtgatacggc tttctggaag caatcataat atatcaaatg gaggaatatt acaaatggga
      721 aatatgcaaa taaaaccaac caggaaaaaa tggaaaggta caatgaccga tcgggaaagg
      781 tttaataacc agatgcatta taaggaaata gaccgttgtt tcaacatgga atttggctac
      841 tggaatgaaa attttaacga atggtctatc tttgtagaaa ataatataat aaataattca
      901 caagctgaca tattttttaa ttttgacata atagaggtag tatctggtaa tgtgtggata
      961 aatccttctt tcccacataa aattatcgaa gagcatgaaa agacaaatat tattatgaat
     1021 tctgacggtc ttatggctga agttccaaaa gacggtcatg atactattcc tcactttatt
     1081 aagtcttcca tagaaacccc tgatgattgg aagaaggtta aaacagaaag gtttaggaga
     1141 gatgacccca ggcgtaaagt tgatgtagaa gaaatcaaaa cggcccatcc tgaaaacaga
     1201 gattatccac tgggagtcaa ttgcggttca atgattggta aaattcgcga tatgttaaca
     1261 tttgaaggcc ttgcatatgc ctgctatgat taccccgata tggttgaaga tatggttgaa
     1321 acatgctgtg tgcttgttga agattttttg gatcaggtat tgccccacat tgattttgat
     1381 tatgctaccg gttgggaaga catatgcttt aagaatggac ctattgtttc tgtagacttt
     1441 tttaaaaatg ttgtaacacc aagatataaa cgaataagta aaaaattgca tgctgtggga
     1501 attgacttat ggtatactga ttgtgacgga gatgtaaggc ctattcttcc ttatttgttg
     1561 gaaggtggaa taaactgtct tttccccttt gaggttaatg gatgttcaca ccctgccgaa
     1621 ctgctttcag aatacggcaa agaattgaga attatgggtg gtgttgacaa gttacagtta
     1681 aagaaaggga gaaaggcaat aaaggaatat ctcaataccc tcacacccct tgttgaaaga
     1741 ggaggatata ttcctttttg cgatcatcgc tgcccacctg atgtaagccc tgatgactac
     1801 ctatattacc tcgatttaaa agaacagatg tttgggttaa aatagctgct ttaatttagt
     1861 catgtgaaga acctacacga agcaggggaa tagttctcct gcttcgtgta ggttcttctt
     1921 cactgctatg aaggtaagtg aaagcagatg gaagcatagg taccacttct aatttccgtg
     1981 tcgtgattta cgaatttttc cttgccttaa acataacaat ccgtaagctt tgttcaaacc
     2041 atatactcaa aaaagcaaaa aatgtttagc actccttttt tgccgtgcct gtcgcgatag
     2101 caactggcaa aaggtaaaaa aggagcggtt aataattttt tgcttttgtt aatcgaataa
     2161 tgccatattt ttggtcatat acaggatata cagaaaatgc gcccaataac aggtttatta
     2221 tttcaaaaac cagaataata tgatatactg tggcagaaga aggaggattt tatgcaaata
     2281 tgtcacgcat aataattaga aaatatcaaa accctgattg taaagatgta ttgaagatat
     2341 gttacaagac aggctatatg ggaaatgatt tggaaagtac aggtaagttt gacgatatga
     2401 agctttttgg ttatttcttt tgcatgtatt atctccttta cgaaagggat cattgctttg
     2461 tcgctgtgga taaagataat aattgtaaag ttgttgggta tattattggc acattggata
     2521 ctaatataca aataagatta tttataaaaa aaatgcttat aagaatagga ttaagaatat
     2581 tatatactat atggaaatat cctgacactt tcaagaatat catgaatatg cttaagaaca
     2641 gaactcggaa atatacgccg aaaagtctat attgcgatta tcccgcccac tttcatataa
     2701 accttttacc cgaatatcag gggatgggca taggtatgtt attattaact gaatttgaag
     2761 tccatgtaaa gggaagtgcg ggtaagggaa tccaccttaa aacttcaaat aaaaattata
     2821 atgcaattaa attttatgaa aaaaacggat atgtgaaatt gttggaattt acagatatat
     2881 tatgggtagg aatagaaaac tacaaaacgg ttatctttac taaaaagcta tgaactgtag
     2941 aagatagaac ctggaaaata aaaccgcaga atgaaatata agagcaagtc catagcggga
     3001 gtttactaaa tatataaaaa atgttacaag cattaagttg tgaaatg
//
LOCUS       DAOSDD010000383         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11390_length_3042_cov_4.348175, whole genome shotgun sequence.
ACCESSION   DAOSDD010000383 DAOSDD010000000
VERSION     DAOSDD010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11390_length_3042_cov_4.348175"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..1505)
                     /gene="thiI"
                     /locus_tag="PK733_19375"
     CDS             complement(324..1505)
                     /gene="thiI"
                     /locus_tag="PK733_19375"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOJ12732.1"
                     /translation="MNEVILVRYGEIMLKGLNRATFERKLVSNIKKSLYGLGPISVER
                     SQGRIYIESIEENYNIEKAIDKLIKIFGIVSVSPAIKINNDFEEIKLHALGLVKKLFE
                     EGKERTFKVETKRGNKKFHMDSREINAELGGFLLDSLPSLSVDVNNPSFMVYVEVREF
                     TYIYTEIIHANGGLPVGTNGKAMLLLSGGIDSPVAAWMMAKRGLEVDAIHFYSYPYTS
                     ERSKEKVIDLAKIISQYCQNINLYIVPFTDIQLEIAEKCPHDQMTIIMRRIMMMIAEK
                     LAAGTGSLALITGESLGQVASQTIQSLSVTNSSVKMPVFRPLIGMDKNEVISLARKIG
                     TFETSILPYEDCCTVFVAKHPQTKPKLESILFSESKLDIDNLINNALKGVEIIRINYG
                     N"
     gene            complement(1587..2747)
                     /locus_tag="PK733_19380"
     CDS             complement(1587..2747)
                     /locus_tag="PK733_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOJ12733.1"
                     /translation="MDKEIYFDNSATTRPFDEVIDYMSYINREVYGNPSSLHTKGIEA
                     ERLMKKSRETIANSLDVSSKEIFFTSGGTEANNLAILGYLRANPRKGRHVLTTKIEHP
                     SILEVFKYLQEEGYSVDYIDVDQNGIIDLDMLENKITSDTALISVMYVNNEMGAIQPI
                     NEIASIRNMCNKETILHVDAVQAYGKLQIKPIKSNINMITFSSHKIHGPKGVGALYKE
                     DKIKIKPIILGGGQETLVRSGTENIPGICGFGLAAEIIHNNIENNWNTVSELNKMFRM
                     MLEENIEEFSIISPQNSSPYILNVSFSNVKGEVLLHHLEEKNIYVSTGSACSSRKNKH
                     SHVLKAMGYKSNKIDGAIRFSFCEFNSKQDIIETINALKEIIPKIRYDKYNQ"
BASE COUNT          900 a          587 c          425 g         1130 t
ORIGIN      
        1 atttattttt taaaattctt gtgcaaaaaa tatgcacatg cgcattagtt tttcaaaaat
       61 ttatcaattt ctctcaaact agctttagtc ctccatgtct ttataaacaa acaactctat
      121 aaataaacta taccaaacta tatataatga ttttgcgcta attttctatt tatgtcaata
      181 ttgcattaag aattgggcgc atcattaata tatgaggaaa tatattccag aagctatcat
      241 tacggtgcct agagccttta aattcactgc aggtaaaatc atgcttgtaa aataacctaa
      301 acaaattcta tattttgagt tatttaattg ccatagttta ttctaattat ctctacacct
      361 ttcaaagcat tatttataag gttgtcaatg tccaattttg attcggaaaa taatatactt
      421 tccaacttcg gtttcgtctg gggatgcttt gctacaaaca cagtacagca gtcttcatat
      481 ggtaatattg aggtttcaaa ggtcccaatt tttctcgcca gactgattac ttcattctta
      541 tccataccta taagaggtct gaacacaggc attttaactg aggaattagt tactgaaagg
      601 ctctgaattg tctggcttgc aacctgaccc aggctctcac cggttatcag tgcaagagat
      661 cctgttccgg cagccaattt ttcagctatc atcatcataa ttctacgcat tataatggtc
      721 atctgatcat gtggacattt ttctgcaatt tccagttgta tatcagtaaa tggcactata
      781 tatagattta tattctggca atattgtgaa attattttcg ccaggtcaat gactttttcc
      841 tttgatcttt cgctggtata aggatagctg tagaaatgga ttgcatcaac ttccaatcct
      901 ctcttagcca tcatccatgc agcaactgga ctgtcaattc ctcctgataa taataacatg
      961 gctttcccat ttgttccaac tggtaagcca ccatttgcat gaataatctc agtatagata
     1021 taggtaaatt cccttacctc tacataaacc ataaaggaag ggttattaac atcaacgctc
     1081 agtgatggga ggctgtccag caaaaatcct cccaactctg cattaatttc tcttgaatcc
     1141 atatgaaatt ttttgttgcc tcttttagtt tcaactttaa atgttctttc ttttccctct
     1201 tcaaaaagtt tctttaccaa ccctaaagca tgcaacttaa tttcttcaaa atcattatta
     1261 attttaattg ccggactgac cgaaacaata ccaaaaattt taattaactt gtcaatagcc
     1321 ttttcaatat tatagttttc ttctatagac tctatataaa ttctcccttg tgacctttca
     1381 acacttatgg gtcccagacc atatagggat ttttttatat tacttacaag ttttcgttca
     1441 aaagtagctc tattaagacc tttgagcatt atttccccat accttaccaa tataacttca
     1501 ttcatactgt tttctccttc cgaaaatgag tcataaattt ataataattt attataacat
     1561 cagtgcattc tgtcaatcgg atttctttac tgattgtatt tatcataacg tattttaggt
     1621 ataatttcct ttaaagcatt aatagtctct ataatatcct gtttgctgtt aaactcacaa
     1681 aaactgaaac gtattgctcc atctatctta ttagatttat atcccattgc cttaagaaca
     1741 tggctatgct tatttttcct tgaagagcat gctgaacctg tagagacata aatatttttt
     1801 tcttcaagat gatgtagaag tacttctcct tttacattac taaaagagac atttagtata
     1861 tatggagatg agttttgtgg cgaaatgata ctaaattctt caatattttc ttccagcatc
     1921 atcctgaaca ttttatttaa ttccgatact gtattccagt tgttctcaat attattatga
     1981 attatttcag ccgcaagacc aaagccgcat atacccggaa tattttccgt tcccgaacgg
     2041 actagtgttt cctgtccgcc tccaaggatt attggcttaa tttttatttt atcttcttta
     2101 tagagtgctc caaccccttt aggtccatga attttatggg agctgaatgt aatcatgttt
     2161 atgtttgact ttataggctt aatttgcaat ttcccatatg cttgtactgc atcaacatgc
     2221 aggattgttt ccttgttaca catatttctt atagatgcaa tttcatttat aggctgtata
     2281 gctcccattt cattatttac atacataaca cttattaatg ccgtatcaga agtaatttta
     2341 ttttcaagca tatcaaggtc aataattcca ttttgatcaa catctatata atcaactgag
     2401 tagccttctt cttgaaggta tttaaaaacc tcaagtattg atggatgctc aattttagtt
     2461 gttaaaacat gccgcccttt tctggggttt gctcttaagt atccaagaat ggcaaggtta
     2521 tttgcctcag ttcctcctga tgtaaaaaat atctcttttg aagaaacatc gagagaattt
     2581 gctatggttt ctcttgattt tttcatcaat ctttcagcct ctatcccctt ggtatggaga
     2641 gatgatgggt tgccatatac ttctctgttt atataactca tataatcgat tacttcgtca
     2701 aatggtcttg tagtagcact attgtcaaaa tatatttctt tatccatatc aactgctgtt
     2761 ccttttcctt tcacttttgc ataaggtttg ttcctaagta ttttaccata taattatact
     2821 tttaaccata tataacttat atgcgggtgc atattgtaaa agtgaagaaa tatttaccag
     2881 gagctgatgt tacactgcct agagccttta aattcagctt cgggcaaaat cgaactcata
     2941 aaattgtctg aactgaattt aagaaacaat tttattcaaa caacgatttt gcttatccta
     3001 cgctcagatt aaaaggctct acagcagctt cacttatgct cc
//
LOCUS       DAOSDD010000384         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11406_length_3039_cov_4.890416, whole genome shotgun sequence.
ACCESSION   DAOSDD010000384 DAOSDD010000000
VERSION     DAOSDD010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11406_length_3039_cov_4.890416"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..782)
                     /locus_tag="PK733_19385"
     CDS             complement(69..782)
                     /locus_tag="PK733_19385"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12734.1"
                     /translation="MREIRKKGMPIGISDYKNLIDRNAYYVDKTLLIKDIINDESETI
                     VLTRPRRFGKTLNLSMLYYYFNNSGDDNTYLFKDKKIFQQGERYISELGKYPVMYLTF
                     KDIKQRTWEYCYNRLISDISSLFTKYRYLKDSDVLFDTEKQDFIDILKGKADIVAYSK
                     SLKILSSALEKYHKEKVMIFIDEYDTPLNAAHTNDYQREAVDFMRELLSGAFKDNNSL
                     KKGVITGINRIAKENIFSG"
     gene            complement(1026..2441)
                     /locus_tag="PK733_19390"
     CDS             complement(1026..2441)
                     /locus_tag="PK733_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HOJ12735.1"
                     /translation="MNTVHPLADIVRLREIGIPKGVCSICSANEFVIEAAFEKAREYG
                     TFVLIEATANQVNQFGGYTEMKPTDFREFVFSIARKANFPLENIILGGDHLGPLVWKN
                     DGKEQAMLKAEELVRDYVLAGFTKIHIDTSIKLSDDPPDQILDTKEIALRAARLCHVA
                     ESAFNELVLINRKAIQPVYVIGSEVPVPGGNEAPIKSGENESGRNESIISGESSKCEQ
                     YEELQITKVQDFEETVNIFYETFRKQGLSSVLQRIVGVVVQTGVEFGDNAICEYNRNT
                     ASQLIAALKRHPGLVFEGHSTDYQTPYKLKQMVEDGIAILKVGPALTFALREVLFALS
                     HIEKELFNSMKNEPYYSKTDIKISQLVEVLDRVMLQQPDNWAKYYTGGIAKQQISRKY
                     SYYDRCRYYLLKPEVMNSITILISNLKCAGIPLPLLSQYLPVQYKKVRNRVLENDPKA
                     IIKDAIGNVIDNYMYATCINT"
BASE COUNT          931 a          559 c          453 g         1096 t
ORIGIN      
        1 ttcatgctct gttataccaa aatactctga atatttttta tcaagtacag tatagacatc
       61 gacattgttt aaccactgaa aatattttct ttagctatac gattaattcc tgttattaca
      121 ccttttttta ggctgttatt gtccttaaat gctccgctta ataattccct cataaaatcg
      181 acagcttctc tttgataatc gttagtgtga gcagcattta aaggtgtatc atattcatct
      241 ataaaaatca ttactttttc cttatgatat ttttccaaag ctgatgatag tatctttaaa
      301 gatttactgt aagctacaat gtcagcctta ccctttagta tatcaataaa gtcttgtttt
      361 tcggtatcga ataagacatc agaatccttt aaatacctat attttgtaaa taaactggaa
      421 atatcactta ttagtctatt atagcaatac tcccatgttc tttgtttaat atctttaaat
      481 gttaaataca tcacaggata tttccctagt tcggatatat atctttctcc ctgttgaaat
      541 atttttttat ctttaaataa ataagtgtta tcgtcacctg aattgttgaa atagtagtaa
      601 agcatggaaa gattaagagt ttttccaaac cttcgtggtc tggtaagtac aattgtttcg
      661 ctctcatcgt tgataatgtc cttaataagt agcgttttat caacataata tgcatttcta
      721 tctattaaat ttttataatc tgatatgcct ataggcatcc ccttttttct tatttctctc
      781 atatcaatat ccaccttttt acatgaataa tttattatat cacttttatt aataacttac
      841 cataaaggta attttatgct aacaaattta cactgatatt ttgcgcttac aaattaagcg
      901 ccaaaaacac tatattggca attctttaaa agaagcctat gataaaccat cgaaagtttt
      961 tgcagggtta ggcgggaata ttgcagtacc cggctttagc ttaataacag cttggtgtgg
     1021 gaacgctagg tgttgatgca agttgcatac atataattat caattacatt tcctattgca
     1081 tcttttataa ttgctttagg atcattctca agcactctat tccgcacttt tttgtattga
     1141 acaggcaggt actgacttaa taaaggtaaa ggaatgcctg cacacttcag attgcttata
     1201 agaatagtaa tagagttcat gacttcgggt ttcagaagat aatatctgca tctatcatag
     1261 taactatatt ttctggaaat ctgttgttta gctataccac ctgtgtagta tttagcccaa
     1321 ttgtcaggtt gttgaagcat tactctgtca agtacttcca ccagttgaga tatttttata
     1381 tcagtcttgc tatagtatgg ctcatttttc atactgttaa ataattcttt ttctatgtgg
     1441 ctaagagcaa ataatacttc tcttaatgca aatgtaagtg caggaccaac cttaagtatt
     1501 gctataccat cctccaccat ctgcttcaat ttatagggag tttgataatc ggtagaatga
     1561 ccttcaaata caagaccagg gtgtcttttt aatgctgcta tcagttgtga cgcagtgttt
     1621 ctattgtatt cacaaatagc attatcacca aattcaacgc ctgtctgaac aacaacacct
     1681 acaatacgct gtaatacact acttaatccc tgtttcctga aagtttcata aaaaatattt
     1741 actgtttctt cgaagtcctg taccttggtt atttgcaatt cttcgtactg ctcacattta
     1801 ctgctttccc cagagattat tgattcgttc cttcctgatt cattttctcc ggatttaata
     1861 ggtgcttcat tccctccggg aacaggcact tcacttccta taacatacac aggttgtatg
     1921 gcttttcgat ttattaatac taattcgttg aaagcacttt ctgcaacatg acatagcctt
     1981 gcggcacgca atgcaatttc tttggtatct aatatttgat caggtggatc atcgcttaat
     2041 tttatgctcg tatcaatatg gatttttgta aagcctgcca gaacatagtc tcttaccagt
     2101 tcctctgctt taagcattgc ctgttcttta ccatcatttt tccagactag tggaccaaga
     2161 tggtcaccac caagtataat gttttctaat ggaaagttag ctttccttgc aatagaaaac
     2221 acaaactctc tgaaatcagt tggtttcatc tctgtatatc caccaaactg gttaacctga
     2281 ttggctgttg cttcaataag tacaaaagtg ccatactccc tggctttttc gaaggctgct
     2341 tctattacaa attcatttgc actgcatatt gagcaaacac ctttaggaat gcctatctct
     2401 cttaatctta ctatatctgc caaaggatgt acagtattca tgctttttca ctccttaaat
     2461 taaatctaat ggtaaattta cttttaacag aaattaaact gaactgctat agctaaatca
     2521 tgcatgatgt caagtcgtgc agaatataaa tgcaagtgtg ctcataaagt atactggtaa
     2581 agcatgatct ttaattactt atacacctta tttgaatata ttggtatgga taaatcacct
     2641 ttaaattaat agtaatctta tacaatatac tactatgtca atttggaaag cgatgtcttc
     2701 cgggataatt tttgatagca tcgggaagat gtattcctta cgggttagca aaaatcaccc
     2761 tccagaagtg cgctttccat tagcaatata acaaagtagt attaaaaagt tgaacaacca
     2821 ttatattata atcgatttat acatagacac attagtatat ctactgcaaa atacatataa
     2881 gaggtgatta tgaatgtacc aatcatataa cgaaattttc acacaatatg aaagccttaa
     2941 gcagacattt aatactgtaa tggcaaaaaa agacgaaatc attggtttct tttcatcagg
     3001 cagcgaggaa gaagctgtat ttgtagcttg tggttcaag
//
LOCUS       DAOSDD010000385         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11423_length_3035_cov_3.237584, whole genome shotgun sequence.
ACCESSION   DAOSDD010000385 DAOSDD010000000
VERSION     DAOSDD010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_3035_cov_3.237584"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..976
                     /gene="recO"
                     /locus_tag="PK733_19395"
     CDS             212..976
                     /gene="recO"
                     /locus_tag="PK733_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ12736.1"
                     /translation="MGYIKTQGLVIKEVNTGEADKIITILSKEYGKMSIIAKGARRAR
                     SRFTACTQFLCYSEFLLFEGREIHTINSCEVIESFSEVGNDLIKLTYAAHIVDLIYDT
                     VQEEQPADKTLKLLLNTLYYLIKTDRSPELITRIFELRYLCILGYAPYVKGCMNCSKD
                     GLENLENLLFSFRKCGFICNSQNCFSADPYALKISSGTARAIYYIVYSHMDKLFNFEL
                     APIVLKELGYIIDRYLKERLEKKYDKLNFLKNIDNL"
     gene            1571..>3035
                     /locus_tag="PK733_19400"
     CDS             1571..>3035
                     /locus_tag="PK733_19400"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12737.1"
                     /translation="MSKRWMRFNALLITIVMVCAVFVSCSSNKDDTGNGSIEGNSTAA
                     TIAGDQTSQGKSSKYPDLKEHVHVDVWGSGEPGTVAAMEKYFSECLGRKFILKSVPND
                     TYNEKVNTMLLSGDYPDTIMLDRIPSQFVQYKQSGQIIPLTEYLNKSEKLKYLIEKQS
                     KVLEDLTYSNEIWGIPYDKFSTKNFWIRQDWLDNLGLKRPETTDEFLDVLRKFKDKDP
                     DMNQKDDTIPLVIAQYLNDFCGFFYAFGANYMFAQDSNGNAIDGFSQPQTKDALQYIK
                     NLMDENFLEKEWITTSYSVLRQKTTAGKVGSSVYWGSRYQWFNVNTIPNVPEARWEMI
                     PFLKGPNGDYGVFQDGVGSPYCITTGSKNPDQAFEFLEWLFGTKEGSWLFMFKETYVP
                     AFYYEKPPEECVYDIKDGKIVPTQYTLNMKQQGIYLGSAYSPLLVFSEQFEPIFPDDP
                     VDEWMKKWIEIQPTVDAAIIPIPKSLDMEDDWYASLYE"
BASE COUNT         1064 a          467 c          591 g          913 t
ORIGIN      
        1 aaaaaaggct tggagttgaa gctttaaatt aaagcacaaa actataaata aattgtaact
       61 atagttttgc atttttttat taaaagcctg attttaagct gttagtatct caatatgaaa
      121 gtggctgatt tcaaggcttt cagcaaaaag tgcaaaacta tagtaataat gcagaactaa
      181 ataataaaat cactatcaat aaggtgggga aatgggatat atcaaaactc aaggattagt
      241 aattaaagag gtcaatacag gtgaggcgga taaaataata actatattat ccaaagagta
      301 tggaaaaatg tctataatag ctaaaggggc aaggagggct aggagcaggt ttactgcttg
      361 tactcaattt ttatgttata gtgaattttt actgtttgaa ggacgtgaaa tccatacaat
      421 aaatagctgt gaagtaattg agtctttttc agaagttgga aatgatttaa taaagctgac
      481 ttatgctgct catatagttg atttaatata tgatacggta caagaagagc aacctgctga
      541 taagacatta aaattacttc tgaatacact atattattta ataaaaactg acagatctcc
      601 tgaattaatt accagaattt ttgagttaag gtatttatgc atattaggat atgcgcctta
      661 tgttaagggg tgcatgaatt gctcaaagga tggattagag aacttagaaa acttattatt
      721 cagttttaga aaatgtgggt ttatctgtaa tagtcaaaac tgtttttctg ctgatcctta
      781 tgcattaaaa atatcttctg gaaccgccag agcaatatac tatatagtat attcccatat
      841 ggataagttg tttaattttg agcttgcacc aatagtatta aaagaattgg gatatattat
      901 agataggtat ttaaaggaga gactggagaa aaaatatgat aagctcaatt tcctcaagaa
      961 tatagacaat ctataaagca tagggtagtt tttataagta tatttatctt ttcaaataga
     1021 ggcatattgt cttcctacaa tccattatat tccgtaaaca atgttcagtc ctttagagcc
     1081 tataaattca cttcaaggca attcaaactt gctaaatgat tttgaataaa aaaagctcaa
     1141 ccggaaaaca ccttttgcag atcatccaga taatcaaggg attggattgc aactgtgcgt
     1201 gactgctttt tcccctatcg caataattgt taacaattga taaataggtc cttccagttc
     1261 tattattggt actttcatta agatacatag ataataagga tggaaggaat gtcaatttta
     1321 acagcaactc aataaaaaag tggaataaag aataaatctg ctacctgtac ataatggatc
     1381 ttattattta taatttttat atagataaca acatgcaact tgtaaaatca acttgttaaa
     1441 atcaacttat taaaagatcg gttagtaaaa gtcaaaagtt gtttcctcct tttcgcagaa
     1501 atattatgat gcttaatcat atttcatact atttagagga ggaacaataa taatgaaagg
     1561 aggagtttga atgtcaaaaa gatggatgag attcaatgca ctattgatta ctattgttat
     1621 ggtttgcgca gtttttgttt catgctcgag caataaggat gataccggaa acggtagcat
     1681 tgaaggaaat tcaacagccg ctaccatagc aggggatcaa acatcacaag gaaaaagcag
     1741 taaatatccg gatttgaaag agcatgtaca tgtggacgtt tgggggtcag gtgaaccagg
     1801 tacagttgct gccatggaaa aatatttttc agaatgcctg ggaaggaaat tcattttaaa
     1861 atcggttcct aacgacactt ataatgaaaa ggtaaacacc atgcttcttt ctggtgatta
     1921 tccagacacc attatgctgg acagaatacc ttcacagttt gtacagtaca agcaaagcgg
     1981 acaaattatc ccactgacgg aatacttgaa taaatccgaa aaactaaagt atcttattga
     2041 gaagcagagc aaagttttgg aagatttgac atacagcaat gaaatctggg gaattcctta
     2101 tgacaaattt tctacaaaaa atttctggat tagacaggat tggcttgata acctgggctt
     2161 gaaaaggcct gaaaccaccg atgagttcct tgatgtactt agaaaattca aggacaaaga
     2221 tccagacatg aatcaaaaag atgatactat accgcttgtt attgctcaat acttaaatga
     2281 tttttgcggt tttttctatg ctttcggcgc caattacatg tttgcacagg acagtaatgg
     2341 gaatgccatt gacggttttt cacagcccca gacaaaggat gcactccagt atataaagaa
     2401 cctgatggat gaaaattttc ttgagaagga atggattacc acatcctatt cggtattacg
     2461 gcagaagaca acagcaggaa aagtcggttc atcagtatat tggggaagca ggtaccaatg
     2521 gtttaatgta aatactatac ccaatgtgcc agaagccaga tgggaaatga ttccctttct
     2581 gaagggaccc aacggggact atggagtttt tcaggatggc gtaggatctc catattgcat
     2641 cactaccggt tctaagaatc ccgatcaggc gttcgaattt cttgagtggc ttttcggaac
     2701 aaaagaaggt tcctggctat ttatgttcaa ggaaacatat gtgcctgctt tttattatga
     2761 gaaaccacca gaggaatgtg tatacgatat aaaagacgga aaaatagttc caacgcaata
     2821 cacactaaat atgaaacaac aaggcattta tttgggttct gcatacagtc cgctgcttgt
     2881 tttcagcgaa cagttcgaac cgatatttcc agatgaccct gtggatgaat ggatgaagaa
     2941 atggattgaa attcaaccca ctgtagatgc agccattatt cccattccca agagtcttga
     3001 tatggaggat gattggtatg catcgctata tgaat
//
LOCUS       DAOSDD010000386         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11424_length_3035_cov_3.221141, whole genome shotgun sequence.
ACCESSION   DAOSDD010000386 DAOSDD010000000
VERSION     DAOSDD010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11424_length_3035_cov_3.221141"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="PK733_19405"
     CDS             complement(<1..1895)
                     /locus_tag="PK733_19405"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ12738.1"
                     /translation="MERFSKYIVNHKIQVIVLFILLLIPSAIGALKTKSNYDILSYMP
                     GDLNSREGEKILEEIFDLSGTGLIVINSKELWEMQKLKKEIESLSGVDSVISFDDFTD
                     IVVPVDFMNSHIRDRFIKGESVLLQVNFKENSRAKGTIDAVKEIRDIIGDKELFGGEP
                     VILDDLQTITSKEMKYYMIIAGISIYIILSLSMTSFLEPLLFLAALGVAIAINMGTNI
                     IKGEISFITASIAAVMQLGISMDYSIFLLHRFEEEKLKFSSIEEAMISAMTKTSVAVA
                     SSALTTIAGFAALMVMKNGIGGDLGFVIGKGVVLSLIVNLTLLPCLILIFNKYADMYR
                     HKPLLPTFRKLSKWIVKWRWAFFIIIILISIPSYFAQRNLEYYYATEHYLPKTTSSAV
                     DTEKISDKFGATEVLYVITKDNGRIKERELIEEVKKIQVVDSVIGMSEQVDMAIPDTF
                     IPKEVSDNFKGGDYGYFQVFMTTPTDDRRTFDAIDKIRQLAAGTYDEYYVTGRAPLAK
                     DLADLVDTDTRNVAIVSISAIVLILALSFMSVSIPLILILVIQLAIWINTSIPYFQGI
                     QVASITPVIIGAIQLGATVDYAILFTSRYRENLESYKRKIDAIRQTIEDTGRSILTSA
                     LTMLSATV"
     gene            complement(2699..>3035)
                     /locus_tag="PK733_19410"
     CDS             complement(2699..>3035)
                     /locus_tag="PK733_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12739.1"
                     /translation="EQLSHVPESFSLLQWFVNRSNLDGDVSHLITGIKKAVAEMDWQL
                     RKDKKTPDLLNVLEKSNYLTAAKIDWLKKEAGKQGLQIEQLLIENFLASAETIQKAKW
                     LLSILKGLI"
BASE COUNT          960 a          589 c          474 g         1012 t
ORIGIN      
        1 acagttgccg aaagcatcgt aagagcactt gttaaaattg agcgtcctgt atcttctatt
       61 gtttgcctga tggcatcaat cttccttttg taactttcaa gattctcccg gtatctgctg
      121 gtaaataaaa tagcatagtc tacagttgct cctaactgaa tagcaccaat tattacagga
      181 gtaatagatg ctacctggat accctgaaaa tatggtatgc ttgtatttat ccaaattgcc
      241 agctgtatta ccaatatcag tatcagcggg atagatacgg acataaagct taaggccaga
      301 ataaggacta tggcactgat tgacacaata gctacatttc ttgtatctgt atctacaagg
      361 tctgcaagat cctttgccaa aggagcacgg ccggtaacat agtattcatc atatgtgcct
      421 gctgccagtt gccgtatttt gtcaatagca tcaaaggttc gtctgtcgtc agttggggtt
      481 gtcataaata cctgaaaata tccatagtca ccgcccttaa agttatccga tacctctttt
      541 ggaataaatg tatcgggaat tgccatatca acttgttcag acatgcctat tactgaatct
      601 accacctgta tcttcttgac ttcctcaatt aattcccttt ccttaattct tccattgtcc
      661 tttgttataa catataaaac ttcagtagct ccaaacttat cacttatttt ttctgtatca
      721 accgccgaag aagtagtctt aggcaggtaa tgctcagttg cataataata ctcaaggttt
      781 ctttgagcga aatatgatgg tattgaaatc agaattatta taatgaaaaa tgcccacctc
      841 cattttacta tccatttaga aagctttcta aacgttggaa gaagaggctt atgcctgtac
      901 atgtcggcat atttattaaa aattagtata aggcatggca aaagtgtaag gtttacaatg
      961 agacttaaca ctacgccttt accaattaca aaacctaaat ctccgcctat tccgtttttc
     1021 attaccatta gagcagcaaa tcctgctatg gttgttagtg cacttgaagc gacggcaaca
     1081 ctagtctttg tcatagctga aatcatagct tcttcaatac ttgaaaactt cagcttttct
     1141 tcttcaaacc tgtgcagaag aaaaatggag taatccatag atataccaag ctgcattact
     1201 gctgctatag atgcagtaat aaaggatatt tcacccttta ttatattggt acccatgtta
     1261 atggcaatag ctacacctaa tgctgcaaga aataacaatg gttctaaaaa agaagtcata
     1321 gataaggaaa gtataatgta aattgatatg cctgctatga tcatataata tttcatttct
     1381 ttacttgtta tagtttgaag atcgtccagt attaccggtt cacctccaaa taattcctta
     1441 tctccaatta tatcccgaat ttctttcaca gcatcaattg taccctttgc tctggaattc
     1501 tccttgaagt ttacctgcag cagtaccgat tctcccttaa tgaatctgtc tcttatatga
     1561 ctattcatga aatctacagg aacaacaata tccgtaaaat catcaaaaga tataacacta
     1621 tctactcccg atagagattc aatttctttt tttagttttt gcatctccca cagttctttg
     1681 ctatttataa caatcagacc tgtacccgat aaatcgaata tctcttccag gattttctct
     1741 ccctctcttg aatttaaatc accgggcata taggaaagta tgtcataatt gctttttgtt
     1801 tttaaagcac caatagctga tggtattaat aataatatga ataatacaat cacttgtatt
     1861 ttatgattaa caatatattt ggaaaatctc tccattagtt ccccctcacc ttcgaaacag
     1921 caatatatgt tcgaaacagc aatatatatt agtatgtctt ttcaacttat tgaatgtatg
     1981 ctttgttgtc attatttaat cacctgtttt aatcacatgt tttaaacaag ccttttaagc
     2041 gcacgattta aatatataat aaatattttc ctttgtcaat aggatttgaa attcttccca
     2101 gagcaatctc tatgactttg tactcggtaa atattaccaa atattgacgg tattagctga
     2161 tttgcatata ataatattag ctgattcagc tgattgttta ctgataaagg agctgatatt
     2221 atgctgatta cttcaaccga tgcaaaaaca actttgacaa gtatatcgga cttgctgcaa
     2281 aagaagatgt cattataacc aaaaacggaa tagccaaact cgtcgcatat aatgaaggca
     2341 atgatgacaa tgatagcaat gatagcaatg atagccaggt ttatattaaa tctttctatt
     2401 tcgaagatct tatagtaagt ctcgaaattt tttcataaaa gcctgccctg atataaatta
     2461 tttgaattat ctcacaatac tagatctcat atatttcgga cagtgctaag tagcgaatca
     2521 tttccagttg caatatttac gacagtgcac tttttcaaaa gtataattta ttcaaacttt
     2581 cgaaaacact acccaatctg aagtgaaaac ggcttattac ttataaacaa attcaaatta
     2641 aaccctttga aattgacagc ttataaaaga gaaacgttat agcaggaggg aaacaggttt
     2701 aaattaaacc ctttaaaatg cttaaaagcc acttcgcttt ttgtatggtt tcggccgaag
     2761 ccaggaaatt ttcaataagc aattgttcta tctgtagtcc ctgtttgccg gcttccttct
     2821 taagccaatc aatttttgca gcagtcaggt aatttgattt ttccagaaca ttcagaaggt
     2881 caggtgtctt tttatccttt cttaattgcc agtccatttc agcaacggct tttttaatac
     2941 ccgttattaa atgtgataca tcaccatcaa ggtttgatct gttgacaaac cactggagta
     3001 atgaaaagga ttccgggaca tgagaaagct gctcc
//
LOCUS       DAOSDD010000387         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11516_length_3015_cov_4.830068, whole genome shotgun sequence.
ACCESSION   DAOSDD010000387 DAOSDD010000000
VERSION     DAOSDD010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11516_length_3015_cov_4.830068"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PK733_19415"
     CDS             <1..1335
                     /locus_tag="PK733_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOJ12740.1"
                     /translation="ASVKFRWNLSGTYQQVLPRYISTDINGGDEREFLQEYFPDPGKM
                     LDAVFLKGYQWPFDPRKISDYGSSLIDILVYNETQLKGRRVFLDYTRNPSWGSNNGQL
                     KFSTLGPEAFKYLENSGALFGTPIQRLRKMNRPAIELYKNNFIDLEKDYLEIAVCAQH
                     NNGGLIGNIWWESNIRNFFPVGEVNGTFGVYRPGGTALNSTQTGSLRAAQYISVNYRG
                     EPLKVEKFVSIAIEQIRERVRLADDFILNSNISINMKSSLNNSDNRADSCKSDCQNSY
                     PDSNVAEIKKKVQKRMTMAGAHIRSYKEVNTTVEESIQDIISLSSNIKISSLKELPEA
                     FRVRDIIISQYIYLSAIKEYLDKGGKSRGSYLVHDDNGMFPADNLPQIFGFSLDNGEL
                     LNVINEIELKRKGNKLEDGFYIVSRWKPVRPIPQSESWFENVWAQYREMSNN"
     gene            1623..2873
                     /locus_tag="PK733_19420"
     CDS             1623..2873
                     /locus_tag="PK733_19420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ12741.1"
                     /translation="MSELALFGGEKVKTTPFGTGKRFGEEELQQLKEALEQDTLFYWA
                     GNKVKQFTKKFANMYGVKHCVATSSGTASLHVALGTIGVTCGDEVITSPITDMGTVIG
                     ILYQNAIPVFADLDPHTYNMDPKSIEEKITDKTKAILVVHLAGNAADMDPIMEIARKH
                     NIKVIEDCAQSYMCYYKGRLVGTIGDIGCFSLNDFKHISVGDGGMVIMNDNQYYEKAL
                     KFADKNYNRLATDPVATRAIQYLAPNYRMNELMGAVAIAQLDKLESICERRNVYGDAL
                     TRGITGLPGIYAHKVLEDGKSSYWFYMLRVDENELGISRNDFSKALAAEGIPNGAGYI
                     PICVYEYDIFTKKNAYPGTDCPFGCKYYGKNIEYYKGLCPTAEEILKTAITIPVKEYY
                     TKQDLEDVIAGIRKVAEYYYKKRK"
BASE COUNT         1085 a          432 c          647 g          851 t
ORIGIN      
        1 gcatctgtga aattcaggtg gaatttatca ggaacttatc agcaggtatt gcccagatac
       61 atttctacag atataaatgg aggggatgag agagaatttc tgcaggaata ttttcctgat
      121 ccagggaaaa tgcttgatgc agttttttta aaaggttacc aatggccttt tgatccacgc
      181 aaaatatctg attatggttc atctttaatc gatatacttg tatataatga aacccagctc
      241 aaagggagaa gagtgtttct tgattatacc aggaatccat catggggcag taataatgga
      301 caattgaaat tttccacact tgggccggaa gcatttaaat atcttgaaaa ttcaggtgct
      361 ttattcggta ccccaataca gagactgagg aaaatgaaca gacctgctat agaattatat
      421 aaaaataatt ttattgacct ggaaaaagat tatctggaaa tagctgtatg cgctcaacat
      481 aacaatggag gacttatagg gaatatttgg tgggaaagca acattaggaa ttttttccct
      541 gtaggagaag taaatggtac atttggcgtt tacagaccag gcggaactgc gctgaattca
      601 acccaaacag gaagcctgag ggctgcacag tatatatcgg ttaattacag aggtgaacct
      661 ttgaaagttg aaaaatttgt aagtattgca atagagcaaa tcagagaaag agtaagactg
      721 gcggatgatt ttattttaaa tagcaacatt agtatcaaca tgaagagcag tcttaataac
      781 agtgataaca gagctgatag ctgcaaaagt gactgccaga atagttatcc agattctaat
      841 gttgcagaaa taaagaaaaa agtccaaaaa agaatgacca tggcaggagc tcatataaga
      901 tcatataaag aagtaaatac aactgttgag gagagtatac aggatatcat aagcttatcc
      961 agcaatataa aaatatcctc tttgaaggaa ttacctgaag cttttagagt aagggatata
     1021 ataatatcac aatatatata tttatccgca ataaaagaat atttggataa aggcgggaag
     1081 agtagaggct catatctagt acacgatgat aatggcatgt ttccggcaga taatcttcct
     1141 caaatattcg gattttcact ggataatgga gaattactca atgtaataaa cgaaatagag
     1201 cttaaaagga agggtaacaa gctggaagat ggattttaca tagtttctcg atggaaaccg
     1261 gtccggccaa ttccccaaag tgaaagctgg tttgaaaatg tttgggctca atatagagag
     1321 atgagtaata attaataaat aaaagtatat tttctgtatt ttaagaaata tatggcaaga
     1381 atagatttct atttagaaag atgcacatta tgaacgaaaa tgctaatgaa aaaatcaaaa
     1441 aagtttagaa gattattata gtctgatcta taattatagt tgtaaataag tactactata
     1501 ccaatttgga aagcgatgtc ttccacgata atttttgata ggaccgggaa gatattgtgt
     1561 ggggaaatgt ctctatacat tgtaaatatt attaaaaaat tatatcagga aggatgaagg
     1621 atatgagcga acttgcatta tttgggggag aaaaagttaa aacaacacct tttggaacag
     1681 gcaaaagatt tggggaagaa gagcttcagc aattgaagga agccttagag caggatactt
     1741 tgttttattg ggcgggtaac aaggttaagc aatttacaaa gaagtttgcc aatatgtacg
     1801 gagtaaagca ttgtgtcgct acatcttcag gaacagcttc actgcatgta gctctcggaa
     1861 ctattggtgt tacctgtggc gatgaagtta ttacttcgcc aataacagat atgggtactg
     1921 ttattggtat actgtatcag aatgctatac ctgtatttgc agatctagac cctcacacgt
     1981 acaacatgga tccaaagtct atagaggaaa agataactga taagacgaaa gcaatacttg
     2041 ttgttcatct tgcaggtaat gcagcagaca tggatcctat tatggagatt gccaggaagc
     2101 ataatatcaa agttattgag gattgcgccc agagttatat gtgctattac aagggcagac
     2161 ttgttggtac aataggagat attgggtgct ttagtcttaa tgactttaaa catatttccg
     2221 ttggagatgg cggaatggtt ataatgaatg ataaccaata ttatgaaaag gctttaaaat
     2281 ttgctgataa gaactataac aggttggcta cagatcctgt tgcaaccaga gcaatacagt
     2341 atcttgcccc caattacagg atgaatgaac taatgggtgc tgtagccata gctcagcttg
     2401 ataaacttga gtcaatatgt gagagaagaa atgtgtatgg agatgccctt accagaggaa
     2461 taacaggttt accgggcata tacgctcata aggttctgga agacggaaag tcttcatact
     2521 ggttctatat gttgagagtt gatgagaatg aattgggaat aagcagaaat gatttttcca
     2581 aggctcttgc tgcagaaggt ataccaaatg gagcaggtta tatacctatt tgtgtttatg
     2641 aatacgatat atttacaaag aaaaacgctt atcccggtac agattgtcct tttggctgca
     2701 aatattatgg aaagaatatt gagtactaca aaggattatg ccccacagcc gaagaaatat
     2761 tgaagacagc tataacaatt cctgtaaagg aatactacac taaacaggat ttggaagatg
     2821 tcattgcggg aataagaaaa gtagctgaat attattataa aaagagaaag tagaattagt
     2881 gggaagatta tgaaaaaagc cggagtcaaa gccccggctt ttttgttgca cccctctgtt
     2941 ctttttaaat atgtataacg caaaaatgat ttaacaaaga acaaaaatga tttaacaaag
     3001 aacaaaaatg attta
//
LOCUS       DAOSDD010000388         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11590_length_2997_cov_5.578858, whole genome shotgun sequence.
ACCESSION   DAOSDD010000388 DAOSDD010000000
VERSION     DAOSDD010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11590_length_2997_cov_5.578858"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..245
                     /locus_tag="PK733_19425"
     CDS             84..245
                     /locus_tag="PK733_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12742.1"
                     /translation="MLKLKDAEYFRKSYLKPALLSGLIEMTIPNKPKSSKQKYRITDL
                     GKKVLKALD"
     gene            313..1173
                     /locus_tag="PK733_19430"
     CDS             313..1173
                     /locus_tag="PK733_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ12743.1"
                     /translation="MNYAFSIENLHFKYPTSKDDTIKGISFNVKEGEIFGLLGPSGAG
                     KSTTQKILIKLLENYQGNIKYFGKDLKSMDKSFYEEIGVGFEMPVHFCKMTALENLKF
                     FSGFYKQTINIQELMERVGLWEDRNIKVGEYSKGMKVRLNFVRSMLNNPRVLFLDEVT
                     NGLDPKNARIIKDIISEFRAKGGTVFLSTHLMNDVEQLCDNIAFCVNGKLNEISTPRD
                     LKLKYGKREVRIEYKENGATHSALFPLEAIGVNEEFQNILKNKEIETIHSGETSLEEI
                     FITLTGVELK"
     gene            1170..1889
                     /locus_tag="PK733_19435"
     CDS             1170..1889
                     /locus_tag="PK733_19435"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ12744.1"
                     /translation="MKCIFRLISGELKRLINYKILPVSLATSVIWIIIFLLISENDAK
                     YIAPLLIYVDVTMMEMIFIGASFHFEKQESTIKSMMVLPVSLGEILSAKVIAAMVLGF
                     ESAIITLAASFFIHGITFNYLMLAIFVIIAGITHGAIGFFLALISKDFTTMLGWMMAY
                     MLPFALPTILFMFEIIDKKYDWMLMVSPSHSASELITSAVTGKYEAGKAIFACVYLII
                     VSIILLRYVIYPKFKDNSVRG"
     gene            1897..2709
                     /locus_tag="PK733_19440"
     CDS             1897..2709
                     /locus_tag="PK733_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12745.1"
                     /translation="MKKCWSLLDKYKFLLKYEAKTITRDPINLYMCFFPIIILLLSTL
                     VFPKIFESIDPLKGATIKYTVLLVLIIILTFSSFFLAAMATFLLIENKDERTLHTIAV
                     TPLGVPGYIKFKMGYIYLMTAMSTIIILLGTKLIAGDKYTIAGIPLFDNINIAEIISF
                     AFVNSLFVPSLSLLEGAFAKNKVEGFAYIKGSGLIALIPALMILETFQGGMQYLLGVL
                     PNFWAVKGMMLELLPTQNNANLSYTVYLLIGAAYNILIFIFAYRLFLKKAEY"
BASE COUNT          965 a          469 c          585 g          978 t
ORIGIN      
        1 tgtaccctat cctgattggt atgaacagaa aaagcagctg aaagatcatt tctggagacg
       61 tctccaggca ggaattacag gatatgctta agttaaaaga tgctgagtat ttccggaaat
      121 catatcttaa acctgcatta ttatcaggct tgatagaaat gacaatacct aataaaccaa
      181 aaagcagcaa gcagaagtat cgcataactg accttggtaa gaaggtgctt aaggccttag
      241 attaacaatg aatggtatca aacaatatgt ggtagttcac gttaccaaca ataacggtga
      301 aaggggatat atatgaatta tgctttcagt attgaaaatc tacattttaa gtatcctaca
      361 tcaaaggatg ataccataaa agggatatct tttaatgtaa aagaaggcga aatatttggg
      421 ctgcttggtc cgtcaggggc tggtaagtct acaacacaaa aaatcctcat aaaattatta
      481 gaaaattatc aaggaaatat aaagtatttt ggtaaagact taaaatccat ggataagtct
      541 ttctatgagg aaattggagt aggttttgaa atgcctgttc atttttgtaa aatgactgct
      601 ttggaaaatc ttaagttttt ctccggcttt tataagcaga ccataaatat tcaggaatta
      661 atggagaggg taggtttatg ggaagacagg aacataaaag ttggagaata ttcaaaaggt
      721 atgaaggtac ggcttaattt tgtccgctca atgctgaata accccagggt acttttttta
      781 gatgaagtca ccaatggtct agatccaaag aatgccagaa tcataaaaga tattatctct
      841 gaatttcggg ccaagggggg gacggttttc ttaagtactc accttatgaa tgatgtggag
      901 cagctttgtg acaatattgc cttttgtgtt aatggtaaac taaacgagat atcaactcct
      961 cgtgatctca aacttaaata tggaaagcgg gaggttagga ttgaatacaa ggaaaatgga
     1021 gctacacata gtgccctatt tcctttagaa gcaataggag ttaatgagga attccagaat
     1081 attctgaaaa acaaagaaat agaaaccata cacagcggag aaacatccct tgaagaaata
     1141 tttattactt tgacaggagt tgagctcaaa tgaagtgtat tttcaggttg atttccggag
     1201 aacttaagcg gttgataaat tataaaatac tgccggtaag ccttgctaca tctgtaatat
     1261 ggataattat ttttctgctt atttccgaga atgatgccaa gtatattgct ccactactta
     1321 tatatgttga tgtgactatg atggagatga ttttcatagg agcatctttt cattttgaga
     1381 agcaggaaag cacaattaaa tctatgatgg tcctgcctgt atcgttaggg gagattctgt
     1441 cagcaaaggt tatagcagct atggttctag gatttgagtc tgctattatt acattagctg
     1501 catcgttttt tattcatgga ataacattca attatctaat gcttgcaatt tttgttatta
     1561 tagctggaat aacccatgga gcaattggat ttttcctggc actgataagc aaggatttta
     1621 caaccatgct gggctggatg atggcttata tgttaccttt tgcgcttcct accatattat
     1681 ttatgtttga aattattgat aaaaaatatg attggatgct gatggtttca ccgtcccatt
     1741 cggcaagtga gttgataaca tcagctgtta caggaaaata tgaagcaggt aaagcaatat
     1801 ttgcatgtgt atacttaata attgtctcaa taatccttct tagatatgtt atatacccaa
     1861 agttcaaaga taattcagtc agggggtagt ttctgtatga aaaaatgctg gtctcttcta
     1921 gacaaatata agttccttct caaatatgag gcaaaaacaa ttactcgaga tccgataaat
     1981 ttatatatgt gctttttccc aattattata cttttattat caacactggt tttccctaag
     2041 atatttgaat ccatagaccc gttaaaaggt gcgactatta agtatactgt tctcttggtg
     2101 cttattatta tactgacttt tagtagcttc tttttggcgg caatggcaac ctttctgcta
     2161 atagagaaca aggatgaaag gaccttgcac acaatagccg taactccatt gggggttccc
     2221 ggatatatta aatttaaaat gggatacatt tatctaatga cagcaatgag tactattata
     2281 attttacttg gaaccaagtt aattgccgga gataaatata caatagccgg tattccacta
     2341 tttgataata taaatattgc agagataata tcttttgcct tcgtaaatag tttatttgtt
     2401 ccttcattgt cgcttttgga aggtgccttt gctaaaaata aagttgaggg ttttgcatat
     2461 atcaaagggt cagggcttat agcgctaata cctgccttga tgattctgga aacttttcaa
     2521 gggggaatgc agtatttgct tggtgtattg ccgaattttt gggctgttaa aggaatgatg
     2581 cttgagctgc ttcctacgca aaacaatgct aatttaagtt atacagtata tttgttaatt
     2641 ggtgcagcct ataatatact catatttata tttgcatatc ggttattctt aaaaaaggca
     2701 gagtattaat gatggcataa tatgagcaat gggcaatagg caatctgcag acgagtttga
     2761 ttttgccaga agctgaattt aaagtctctg agcagcggaa tgacagcttc tggtaaatat
     2821 ttcttcactt ttagtaaatg cacccgaagt ttattgtgac acttctctta aaatagcttt
     2881 ttatatttat tttccttata cccagtaact tctaccataa cctcagccaa agttttgcct
     2941 tcatttctca tatcatcaat ataaaagtct gctttaatac ggccgtaacg cagaatt
//
LOCUS       DAOSDD010000389         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11599_length_2996_cov_3.220333, whole genome shotgun sequence.
ACCESSION   DAOSDD010000389 DAOSDD010000000
VERSION     DAOSDD010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11599_length_2996_cov_3.220333"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PK733_19445"
     CDS             complement(<1..891)
                     /locus_tag="PK733_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOJ12746.1"
                     /translation="MVIAKRIFMGGVAVPHRKNTAQYETVNMSVPEKVVIPMLQHIGA
                     PCQPTVKKGDLVKVGQVIGTSGKYVSAPIHSSVSGTVANIVQVIHSSGIYVDAVEIKT
                     DGNQDIHESVKPPVYSNDAELLELIKQSGLVGLGGAGFPAHVKLSVQPDKKIDTLIVN
                     GAECEPYITADYREIMENSWNIISGINILMDLLKVDNVLIGIEDNKPDAIKVLTDLAN
                     TSEKINVVTLKSRYPQGAEKMLIYSIIGRKVPPGKLPLDVGVVVMNVNSVSFVAEYVK
                     KGMPLIKKRVTVDGPAVANPS"
     gene            complement(1808..>2996)
                     /locus_tag="PK733_19450"
     CDS             complement(1808..>2996)
                     /locus_tag="PK733_19450"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ12747.1"
                     /translation="EGISVKVKKILVRDINKKRNIDIDQGLLTDNIEDIIENPDISLV
                     AEFMGGEEPARQYILNALKNKKTIVTANKEVMARHWAELESTAKENGVGLYYEASVAG
                     GIPIIKVIRESLQANNIFEIMGIINGTTNYILTKMSEEGRSFESVLLEAKELGYAEPD
                     PTSDIEGFDPMFKLSILSTLAFHTRVHVERIYTEGITGITIEDINFGRELGYAIRLLA
                     IAKKRGREIEVRVHPTFIPLDHPLAAVRDSYNGIFIRGDAVGSLMLYGRGAGDLPTGS
                     AVISDIINACLNKSRHTYSTLCNGDWVSDTIHFNDNWETEYFIRMTVKDKPGVLARTA
                     GIFGKHDVSIASVIQKGRNGDSTPIIFVTHKAKELSIREAIKDMINEPVVVRVENVIR
                     VES"
BASE COUNT          866 a          617 c          436 g         1077 t
ORIGIN      
        1 tgaaggattt gcaactgccg gaccatctac cgtaaccctc ttttttatta gtggcatacc
       61 tttcttgaca tattcagcta caaatgaaac actatttaca ttcattacca caacacctac
      121 atcaagagga agttttccag ggggtacctt tcttcctatt attgaataga taagcatttt
      181 ttctgcaccc tgggggtaac gcgatttaag cgtaacaacg ttgatttttt cacttgtatt
      241 ggcaaggtct gtcagtactt ttatagcatc tggcttatta tcctcaattc ctatcaaaac
      301 attatcgact ttgagtaaat ccataaggat attaataccg ctgataatat tccatgaatt
      361 ctccattatt tccctgtagt ctgcagttat atacggttcg cattccgctc cgtttactat
      421 taaggtatct atttttttat ccggttggac agaaagcttg acatgggctg gaaaacctgc
      481 accgccaagc cctacaagac ctgattgctt aatcagttca agcagttccg catcattgga
      541 atatacggga ggttttacac tctcgtgaat gtcctgatta ccatctgttt ttatttccac
      601 agcatcaaca taaatccctg atgaatgaat tacctgaact atatttgcta ctgttcctga
      661 tacacttgaa tgaattggag cagatacata tttgccggat gtacctatga cctgtcctac
      721 ttttactaaa tcaccttttt ttacagtagg ttggcaaggt gcgcctatgt gctgaagcat
      781 aggtattact actttttccg gcacactcat atttacagtt tcatattggg cagtattttt
      841 tctatggggt actgccacac ctcccataaa tattcttttc gcaattacca tattattgac
      901 ctcattttca aatgcttttg atatttatat tatatatcgc attatgtatc gcattatgta
      961 tcgctttgta tttcgcttta tgtatcactt aatgtatctc tttgtatttc gctttgtatt
     1021 tcgctttgta tattattttg tatatcattt tgtataccac tttgcgtatc actaattgat
     1081 atgttgctat atggtaatca cttcagtttt acacttattg caacaagtgt aagcataaat
     1141 ttagaatatc aaaatatatg gcaatcactt cagttttaca cttaccataa ttatatttat
     1201 aagaactata cttacaagtt ttttatttag tatttgctgc aaaataaaaa tccataatgc
     1261 tgcaagtaca aaatgtacaa tattgcaaag tataattgag gcatattatc cctcaatttc
     1321 tttccccacc gagctttttt tacccttaaa tgacacttgc aatattgtgg aatttctact
     1381 tgcaactttc ggtaaaaagt acttacataa aacattattt aacttaatac aatcaaattg
     1441 catcatccat aattataatt attactaggt aataagtcaa atatataaaa ggtatataat
     1501 taaggatgaa catgtattat ttaatcattt cattagttta ttattgtttt tcttgtaatt
     1561 ttgctgccgc actatacatt atactaattt aaaatattta aatatatctg tatttgttta
     1621 taatagtgca ttataggcat tcatcaatgt attttgtata caatttataa tatggttggt
     1681 atggaaacca ctgattaagt cgggatgtcg aatcagtgcg tatccttcaa aatgcaagat
     1741 aaagtactgc aaatttcaat gtgttgatac tgaatgattt ttttacacca gaatcaactt
     1801 ttacttttca actttcaact cttataacat tctctaccct taccacaacc ggctcgttta
     1861 tcatatcttt tatggcttcc cttattgaaa gttcttttgc tttatgagtt acaaatatta
     1921 ttggcgtact gtctccgttg cggccttttt gtataaccga agcaatacta acatcatgct
     1981 ttccaaaaat acctgctgtc cttgcaagaa caccgggctt atctttcact gtcatacgga
     2041 taaaatactc tgtttcccaa ttatcattaa aatgaatcgt atccgaaacc caatcgccat
     2101 tgcataaagt agaataagta tgccttgatt tattaaggca ggcattgatg atatctgaaa
     2161 taacggcact tccggtaggc agatccccgg ctcctctgcc gtagagcatc aggctgccta
     2221 ctgcatcacc cctgatgaat ataccattat atgaatcccg taccgcagcc aatggatggt
     2281 ctaaaggaat aaaagtggga tgaacccgta cctcaatctc ccgtccgcgt ttcttagcta
     2341 tagctaaaag tcgaatcgca taacccagtt ccctcccaaa atttatatct tctatagtta
     2401 tgccggtaat accctctgta tatattcttt ccacatgaac tctggtatga aaagccaggg
     2461 ttgaaagaat agataatttg aacatgggat caaatccctc tatatcggaa gtaggatcgg
     2521 gctctgcata tcccaactcc ttggcttcca gtaaaacact ctcaaagctt ctgccttcct
     2581 ccgacatttt tgtaagaata taattagttg ttccgttaat tatccccata atctcaaata
     2641 tgttattggc ttgcaatgac tctcttataa ctttaataat cgggatcccg cctgcaacac
     2701 tggcttcata gtataatcct accccatttt cttttgcggt actttccaat tcagcccaat
     2761 gccttgccat aacttcttta tttgctgtaa ctatagtctt cttattttta agggcattaa
     2821 gaatatactg tcttgccggt tcctcacctc ccatgaattc agcaactaat gaaatatcag
     2881 gattctcaat aatgtcttct atattatctg taagcaaacc ttggtctata tcaatattcc
     2941 tctttttatt aatatccctt acaaggattt ttttcacctt tacactaatg ccttct
//
LOCUS       DAOSDD010000390         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11731_length_2969_cov_4.516815, whole genome shotgun sequence.
ACCESSION   DAOSDD010000390 DAOSDD010000000
VERSION     DAOSDD010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11731_length_2969_cov_4.516815"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>2969
                     /locus_tag="PK733_19455"
     CDS             192..>2969
                     /locus_tag="PK733_19455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOJ12748.1"
                     /translation="MLNKNNINCILLCGGKGTRMQSETKHKVCFEIDGVPAILRSLDK
                     FYDAGIGRFIVVVGALPGQVIECIGGKYPNAAFVYQKEQKGTGNAALIGYKIVEKFNV
                     DGPVIITMGDKITDSTFIRRVVKKFQDTGADFLVSAQPKETNPHGGRIVLDETGKPVG
                     IREEIDTKRAIIYKKVLDMLDEKAAFIQDILQEQNNIKDIGQSTGQSIDQNIGKNAVN
                     NINHNIPQSIAQNIIHGIEKYAVSVISNSKKRDKIVSEISSLDYSNIENLRKIVEERA
                     IIKIGGREFDAEYVDNSKYTNASFYMLSNKAAQYAFPLLNDNNAQKEEYITDIVEILA
                     STGEFKLEFEAIKEPYEIMGFNNVEELLKIEDYYRHLRTDNGLTPDERCYKPVKEWID
                     LFENADKDKLLYNCLAEMYGEDDALISERKKAYLDVLKLFADKYGAERKTIISRAPGR
                     INIMGRHIDHRGGNVNVMSINKEVIAVAAVRDDDRIRIINTNGDFKERDFCISEHLIE
                     LDWDSWLSYLESGEIEKMVINAKGDWANYVKAPILRLQYQFRDRKLKGMDMAFTGNIP
                     IAAGLSSSSAIVVSTAEAFHVLNNLNLTPQKFVELCGEGEWYVGSRGGAADHAAMKFA
                     KRGHVVKLGFLPFNFEDMYSFPEGYKLIIANSHVKSNKTTNSKDAFNSRIASYEFGVM
                     IFKEKFPQFKGVVKHLRDINTNKLGLPPSKIYELLLQLPEKVTPDELFDLLCGGKGWN
                     QEYRGNQEDQSNWEKQENQENCTNQEGQGNWWDQESQKSYESHENYKKIKRILASHNP
                     PDYYYVRTVTMFGVAECCRARLCKELLDAGKIVEFGKMMNTSHNGDRAVYYDKNGTMH
                     DYDWGLSDAKLEELINDLRSENPERVLRAQIEKQPGGYACSVPEIDYIVDVALGVDGV
                     MGAQLSGAGLGG"
BASE COUNT         1053 a          440 c          682 g          794 t
ORIGIN      
        1 actggcgttt gtagggcctg gtactggcct gtcggttgcg caaggcaccg atcagccgtt
       61 tatagtgctg ggtactgccc gatgtatgcc gaaatatatc taattaagtc gaatgttgta
      121 gaatcatgtc aaataatgac ggtatatcca ttagtcaaaa aaatatataa atttgtgagg
      181 gaatagatac aatgttaaat aaaaataaca ttaactgcat attactgtgt ggaggaaaag
      241 gaacaagaat gcagtcggag acaaagcata aagtatgctt tgaaattgat ggtgttcctg
      301 ctatattgcg ttctcttgat aaattttatg atgcgggcat tggacggttt atagtagtgg
      361 ttggcgccct tcccggacaa gtcatagaat gcattggggg taaataccct aatgctgctt
      421 ttgtgtatca aaaggaacaa aaaggtacag ggaatgcagc attaattggc tacaaaattg
      481 ttgagaagtt caacgtagat ggaccggtca taattaccat gggtgataaa atcactgatt
      541 ctacttttat tagaagagta gttaaaaagt ttcaggatac aggtgcagat tttcttgtat
      601 ctgcacaacc caaagaaaca aatcctcatg gaggaagaat agtacttgat gagactggca
      661 aacctgttgg tattagagaa gaaatagaca ccaagagagc aattatttat aaaaaggtgc
      721 tagatatgct tgatgaaaaa gcagcattta tacaggatat tttgcaggag cagaataata
      781 taaaagatat tggtcagagt actggccaga gtattgatca gaacattggt aaaaatgccg
      841 taaataacat taatcataat attcctcaaa gtattgctca aaatatcata catggcattg
      901 agaagtatgc ggtgagtgtt atttcaaata gtaaaaagag agataaaatt gtaagtgaga
      961 taagttcact ggattatagt aacattgaaa acttaagaaa aattgtggaa gaaagagcta
     1021 taataaaaat tggcggtcgg gagtttgatg cagaatatgt tgataattct aaatatacta
     1081 atgcaagttt ctacatgctt agcaataaag ctgcacaata tgctttccct ttgctaaacg
     1141 acaataatgc acagaaggaa gagtacataa ctgatatagt tgagatactt gcttcaactg
     1201 gtgagtttaa attggaattt gaagctataa aagagcctta tgaaataatg ggtttcaaca
     1261 atgtagaaga gctcctgaag attgaagatt actacagaca tctgagaaca gataatggtc
     1321 ttacaccgga tgaaagatgt tataagccgg taaaagaatg gatagattta tttgaaaatg
     1381 cggacaaaga taaactgcta tataattgcc ttgctgaaat gtacggtgaa gatgatgcgt
     1441 tgataagcga gaggaaaaaa gcttatcttg atgtacttaa gctttttgca gataaatatg
     1501 gagcggagag aaaaacaata atttccagag cccccggacg tataaacata atgggaaggc
     1561 atatagacca tcgcggcggc aacgttaatg taatgtccat caataaggaa gtaatagccg
     1621 ttgctgctgt aagagatgat gatagaatca gaataattaa cacaaatgga gatttcaagg
     1681 aaagagattt ttgtatatca gagcatctga tagaacttga ttgggatagc tggttaagtt
     1741 atcttgaatc gggcgaaatt gagaagatgg ttatcaatgc aaagggtgac tgggcaaact
     1801 atgtaaaagc accaatatta aggcttcaat atcagttccg ggacagaaag cttaagggta
     1861 tggacatggc ttttacaggt aatattccca tagcagcagg tttgtccagt tcatcagcta
     1921 tagttgtatc taccgcagaa gcttttcatg tattaaataa tcttaactta acaccacaaa
     1981 agtttgttga attatgtgga gagggtgaat ggtatgttgg ttcaagagga ggggcagccg
     2041 accatgctgc aatgaaattt gcaaagcgtg gtcatgttgt aaaactggga ttcctgccat
     2101 ttaattttga agatatgtac agcttccctg aaggttacaa acttatcata gccaactccc
     2161 atgtaaaatc aaacaaaaca acaaattcaa aagatgcttt taatagtaga atagcttcat
     2221 atgaatttgg agtaatgatc tttaaggaaa aatttccgca attcaaaggt gttgtaaagc
     2281 atcttaggga tataaacaca aacaaacttg gacttccccc aagtaaaata tatgaactgt
     2341 tgctgcagtt gccagaaaag gtaacaccag atgaattatt tgatttgttg tgtggtggaa
     2401 aaggttggaa tcaagaatac cgagggaatc aagaagacca aagtaattgg gaaaagcagg
     2461 agaatcagga gaattgtacg aatcaagagg gacaaggaaa ttggtgggat caagagagtc
     2521 aaaagagcta tgaaagtcat gaaaattata agaaaataaa acggatactg gcatcacata
     2581 acccacctga ctattattat gtgaggactg ttaccatgtt tggtgtagcg gaatgttgca
     2641 gagcaagact ttgtaaggaa ctccttgatg caggaaaaat cgtggagttt ggtaaaatga
     2701 tgaacaccag ccacaatggt gacagagctg tatattatga caaaaatggc acaatgcatg
     2761 attatgattg gggcctatcc gacgcaaaac tcgaagaact cataaatgac ctaagaagtg
     2821 agaaccccga aagagtcctc agagcacaaa ttgaaaaaca acctggcgga tacgcctgct
     2881 ccgtccccga aattgattat attgtagatg tagctttagg cgtggatggt gtcatggggg
     2941 cgcaactttc tggagcaggc ctcggcggc
//
LOCUS       DAOSDD010000391         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11739_length_2966_cov_5.306080, whole genome shotgun sequence.
ACCESSION   DAOSDD010000391 DAOSDD010000000
VERSION     DAOSDD010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11739_length_2966_cov_5.306080"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PK733_19460"
     CDS             complement(<1..1336)
                     /locus_tag="PK733_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12749.1"
                     /translation="MYNNVGKWKIVHFNIPEDTEEIEDVLRNILDKYHCRYKYLIQNV
                     AIFDTEWVFEQDVQSNIQKEIASLNKDGKTEDAIYESEDDSSLKIEELEEILFHLSLE
                     GLSEEDFITCLQSMDDYMVFIYESEMEMLEKIDYNPDTFDDKPLIHNIRKKNTAVLVS
                     QTNPWNFAFVGYMKILDELKVTIKTFQYEILQDIHEYFNSKEIRAVWEDNLKWKWLFS
                     RKTGQKYRATDQHIKDNSLMKDFLKTTKSTKLAAKFSYLLKKLLDKGYLEYSEYMELE
                     KIECDECNTDEMRDINKFLQPYTRIVEDDYPSSAMWIDTATGIIFSQSEEKTDPKRIS
                     EYIKTSRIYAPTHYLLHYIRAFWLQDFVKDIFIKIKKKWEDENAELEIISIETDIQFD
                     LKTVGNPGNPSRDIDVLVRILNISSKQEYIIAVEAKRYGNEFHQVKKEIQSKI"
     gene            complement(1358..>2966)
                     /locus_tag="PK733_19465"
     CDS             complement(1358..>2966)
                     /locus_tag="PK733_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12750.1"
                     /translation="SQPPKRPSFIVNQEDAKKHIVTEMANCAQNNMFSYIVLSGDVGD
                     GKTLFLNWIYRYFSEHKEFHMIKFRVEESSTVKYSFIKMLVAEVFKRYNNTFNEALKN
                     AFKIIDDNLPEDEYIGKIIEEYHVSNELARTVYEFHRNGKATMPAFRVLGACYGKTEL
                     AKLKINNLKDTDYHKVFEWFQEYRITKGLIIILLDEFEHTYLSLTPAMRKSFMLSYKN
                     FIDRTAADASRKTVLIAASTEQYQGQLEKGVSDIDRALWSRLNGHIVYLAQFRPADDK
                     NVSQLFDELRKRYEKAYDINLPDVNPSTIRKHLHFKLGGDTTLECSYRIAVSSLLVIL
                     EELRSAPSSTLDSQDDKHMKEENHEHYKIIQQQYQKLIEETIKDWDDTHYNNRPGKIK
                     SILELLFNEHKYTVKPVPGLNKQRVDALFVEPKVNGKPNKFIFIAIATTAKSLTSKLI
                     RCNNIRDELVKAVPEIDFQTYFIYQANLANKGFKLELNKNTHIFPIEFESEFYDLLAL
                     NTVKDENVKQILLSNLKDTLAKLDISI"
BASE COUNT          896 a          552 c          383 g         1135 t
ORIGIN      
        1 taattttaga ctgaatctcc ttcttaactt gatgaaattc atttccatac cttttggctt
       61 caacagcaat aatatattct tgttttgaag agatattcaa tattcgtaca agaacatcaa
      121 tgtctcttga tgggtttccc gggtttccaa ctgtcttcag gtcaaattga atatcagttt
      181 caattgagat aatttccaat tccgcatttt catcttccca ctttttcttt atctttataa
      241 aaatgtcctt aacaaaatcc tgaagccaaa atgctcttat ataatgtaat aaataatgtg
      301 ttggagcata aattctgctg gtttttatat attcagaaat gcgcttaggg tctgttttct
      361 cttcactctg ggagaatatt atccctgtag ctgtatcaat ccacatagca gaagacggat
      421 aatcgtcctc aacaatacgt gtatacggtt gtaaaaattt attaatatct ctcatttcat
      481 cggtattaca ttcatcacat tcaattttct ctaattccat atattcgcta tactcaagat
      541 atcctttatc tagtagcttt ttaagcaaat aactaaattt agcagctagc tttgtactct
      601 ttgtagtctt caggaaatcc ttcataagag aattatcctt tatatgctga tcagttgccc
      661 tgtacttttg ccctgttttt cggctaaaaa gccatttcca cttaaggtta tcctcccata
      721 ctgcccttat ttccttactg ttaaaatatt catgaatatc ttgaagaatt tcatactgga
      781 aagtttttat tgtcaccttt aattcatcta gtattttcat gtatccaaca aaagcaaaat
      841 tccatggatt tgtttggctt actaaaactg cagtattttt ctttcgaata ttgtgaatta
      901 atggcttgtc atcaaatgta tcaggattat aatctatttt ctccagcatt tccatctcag
      961 actcatatat aaataccata taatcatcca tactttgtaa gcaggtaata aaatcttcct
     1021 ctgataatcc ttcaagtgaa agatgaaaga gtatttcctc cagctcttct atttttaaac
     1081 tactgtcgtc ttcactttca taaatagcat cttcagtttt gccatcttta tttagtgatg
     1141 caatttcttt ttgaatattt gattgaacat cctgttcaaa gacccattct gtgtcaaata
     1201 tagcaacatt ctgtatcaag tatttatacc tgcaatgata tttgtctaaa atattccgta
     1261 atacatcctc tatttcttct gtatcttctg gaatattaaa atgcacaatt ttccatttac
     1321 ccacattatt atacattatt aattacccct taatttatca tatgcttata tcaagcttag
     1381 caagagtatc ctttaaatta cttagtaaaa tttgctttac attttcatct tttacagtat
     1441 ttaaagctaa taaatcataa aattcacttt caaattcaat agggaaaata tgtgtattct
     1501 tatttaattc taatttaaaa cctttattag ctaaatttgc ctggtaaata aaataagttt
     1561 gaaaatctat ttctggaact gccttaacca attcatctct aatattattg catcgaatta
     1621 actttgaagt taagcttttt gctgtagttg caattgcaat aaaaataaat ttattaggtt
     1681 ttccgttaac ctttggttca acaaataaag catcgactct ttgcttatta agacctggta
     1741 ctggtttaac agtatactta tgttcattga ataaaagttc aagtatagat tttatctttc
     1801 cgggacgatt attataatgg gtatcatccc aatcttttat ggtttcttct ataagctttt
     1861 gatattgctg ctgtataatt ttataatgtt catgattctc ctctttcatg tgtttatcat
     1921 cttgtgaatc aagtgtagaa cttggcgctg atcttaattc ctccagaatg actaaaaggc
     1981 ttgatactgc tattcggtag ctgcattcca gggttgtatc tccccccaat ttaaaatgta
     2041 agtgtttacg tatagtagaa gggttaacat caggaaggtt aatatcataa gctttttcat
     2101 atctcttcct cagctcgtca aataattgcg acacattttt atcatcagca ggtctaaatt
     2161 gggctaaata tactatatgt ccattcaatc ttgaccaaag tgccctatca atatctgaaa
     2221 ctcccttttc aagttgacct tgatattgct ctgtagaggc agcaattagt actgtttttc
     2281 tggaagcatc tgcagctgtt ctatcaataa aattcttata gcttaacata aaactttttc
     2341 tcatagctgg agttaaagat aaatatgtat gctcaaattc atctaacaga attataataa
     2401 gtccttttgt tattctatat tcctgaaacc actcaaaaac cttatgatag tccgtatcct
     2461 taagattatt aatttttagt ttagccaact ctgtcttacc ataacaagct ccaagaaccc
     2521 taaaagccgg catagttgcc ttaccattcc gatgaaactc ataaactgtc cgtgccaatt
     2581 cattgctaac atggtattct tctataattt tccctatgta ttcatcttct ggtaaattat
     2641 catctataat cttaaatgca ttttttaatg cttcattaaa tgtattatta taccttttaa
     2701 atacctctgc tactagcatt tttataaaac tatattttac agtggaggat tcttctactc
     2761 ggaatttaat catgtggaac tccttatgtt cactgaaata ccgatatatc caatttaaaa
     2821 agagtgtttt cccatctcct acatcacctg atagcactat ataggaaaac atattgttct
     2881 gagcgcaatt tgccatttca gtaacaatat gttttttagc atcttcctga ttcactataa
     2941 aactcggtct cttcggaggt tgggaa
//
LOCUS       DAOSDD010000392         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11784_length_2958_cov_3.018601, whole genome shotgun sequence.
ACCESSION   DAOSDD010000392 DAOSDD010000000
VERSION     DAOSDD010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11784_length_2958_cov_3.018601"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            769..1209
                     /gene="fabZ"
                     /locus_tag="PK733_19470"
     CDS             769..1209
                     /gene="fabZ"
                     /locus_tag="PK733_19470"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOJ12751.1"
                     /translation="MLSNIEVQNIIPHRYPFLLVDKVLEVEPGKRAVAVKNVTVNEPF
                     FQGHFPGYPIMPGVLIVEALAQTAGIAAASLEENRGRLGVLTGIDSMKFRKQVVPGDV
                     LKLDAEILMLKMGMAKVKVLATVDGQTAAEGEIKFAMVNTEKQK"
     gene            complement(1686..>2958)
                     /gene="murC"
                     /locus_tag="PK733_19475"
     CDS             complement(1686..>2958)
                     /gene="murC"
                     /locus_tag="PK733_19475"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ12752.1"
                     /translation="SNITQKLESKGAVIYYNHDKGNINNPDAVIYTAAIKDNNPELVK
                     ARSLDIPIIDRATLLGLLMKKYPHSIAVSGTHGKTTTTSMIAMVMLEYGLDPTVHIGG
                     ELEYIGGTTRIGGNDYFVTEADEYYESFLKFYPYIAVILNIEFDHADYYKDIEHVKSS
                     FLKFTNLVPKDGYVVVCADDISISSIMDKITANKITYGINTDTYTWSAKNILFDMSGC
                     AVYTLLKEGKEIDSIKLNVPGIHNVYNSLAAIAASYLAGCSIVGIKRGLCKFTGTHRR
                     FELKGIFNNIKVIDDYAHHPSEIKATLKAAKGSNHSRIWCVFQPHTYTRTKTLLNEFA
                     VSFSDADTVIVSDIYAARELDNGEINSKALVGKINEAGGRAIYLKDFDTITEYLKNSS
                     LPGDLIITMGAGDIYKVGELLLKETHTKAVV"
BASE COUNT          934 a          517 c          557 g          950 t
ORIGIN      
        1 aatctaatat ggttatccaa cctttgtaag aaggttagat agccatatta ttatatcaga
       61 ttaatacaaa ctttgacaac gaaggggaaa taaatgcacc tacttggacc ttggagctgg
      121 gcgcattttc tgtatatgac caaaaaagaa aatacgcccc ttggggcttg gacttgtact
      181 ttagcttgtg ttttggtata ttatgattgt tccccaaaaa gtcgttttcg actttccact
      241 gcaggattac ctgcagtgag ttgcaacttt gttgcaacct ggtgcaatct aatatggtta
      301 tccagctttc ttgtgaagac tgatgaaatt cgaggtgcct taaatttcat cagaaagtca
      361 tttcatgact ttctgggtaa taaccatatt attgattaaa attattattg tgaaaagctg
      421 cttatatgga aaaactattg ttttgcattt tatattaaaa gccctaattt taagcctctc
      481 cttgtaaaat acaggtggct tgaattcagc attctcatag aaaagtgcaa aactcaagga
      541 aaaatataaa aacaaaaaag tcaatgaagt aaattaccag ttaaatgctg gaaaaataaa
      601 agtctctgac acgggctcta cggctgtgta caagaactat tatacaagga ggcaataaag
      661 cgtgtaaatg cgttatatcc atgaggagct cttttaaaaa aaagataaaa ttctaagaat
      721 taacgagaaa acccaagaaa attaaagata atagagggag gatacactat gttatcaaat
      781 attgaagttc aaaatatcat accccacaga tatccttttt tgcttgtgga taaagtactg
      841 gaagtggaac ccggtaaaag agcagtagca gttaagaatg ttacagtaaa tgaaccgttt
      901 ttccagggac attttccagg ataccctatt atgccaggag ttttgattgt tgaagcactt
      961 gcccaaacag cagggattgc tgctgcttcc cttgaagaga atagggggag gcttggtgta
     1021 ttaaccggca tagattcaat gaaattcagg aaacaggttg ttccgggtga tgtcttaaag
     1081 ttggatgcag aaatccttat gcttaaaatg ggcatggcaa aagttaaggt attagctact
     1141 gttgatggtc agacagcagc agagggtgaa ataaagtttg ccatggttaa tactgagaag
     1201 cagaaataga gagtctgtta ccgtgattaa tgccgggaaa caatgtgatt aatctaggaa
     1261 atacactgga aatacttact gtaaacttct acttagcatg taactatgca ggggtacgga
     1321 acagagcaca cgcgacacac atagggacaa cagggacgat acgcatagag atggttcttg
     1381 tgtgtcctta tgtgtgccat gtgacaaata atgtacatct ccatttcgca catttatact
     1441 tatgtttgca tgtgtgatca gcatactcat aatacatgcg ttcgctgatg gcgcacattt
     1501 atgcttatgt gtgcatgtgt gcctgaatag ttaccttaac atgatagtta gcttaaaagc
     1561 ctgttgtagc atgacatagc cactcatgct tttggcatac gtagcatagg gtatttatta
     1621 taatttgcaa taatcagagc tgttttacag ttgtttcata tttcagcaca ctaaagcaag
     1681 aatggttata ctactgcttt tgtatgcgtt tccttaagga gtaattcccc caccttatat
     1741 atatctcctg cacccatagt tattatcaaa tcacctggta atgagctatt ttttaaatat
     1801 tctgtgatgg tgtcaaaatc cttcaaatat atggcccttc ctcctgcttc atttattttt
     1861 cctacaagag cttttgagtt aatttcaccg ttatccagtt ccctggcagc ataaatgtct
     1921 gatacaataa cggtatcagc atctgaaaaa gaaactgcaa attcgtttag aagggtttta
     1981 gttctggtat atgtatgggg ttgaaaaaca caccatattc tggagtgatt actgcctttt
     2041 gcagccttta acgtggcttt tatttctgaa ggatgatggg cataatcatc aataacttta
     2101 atgttattga aaatgccttt taattcaaaa cgcctgtggg ttccggtaaa cttgcacaag
     2161 ccccgtttta tgcccacaat gctgcatcct gcaagataac tggctgcaat agcggcaaga
     2221 gaattataaa cattatgtat tcctggaaca ttaagcttaa tagaatcaat ttctttacct
     2281 tcttttaaaa gggtataaac agcacatcct gacatgtcaa aaaggatatt tttggcactc
     2341 caggtgtaag tatccgtatt tattccatag gttattttgt tagctgtgat tttatccata
     2401 atagaagata tgctaatatc atctgcacat acaaccacat atccatcctt tggaacgagg
     2461 tttgtaaatt tcaaaaaaga acttttaaca tgttcaatat ctttataata gtcggcatga
     2521 tcaaattcaa tatttaatat aacggctatg taaggataaa atttaagaaa gctttcatag
     2581 tattcatcag cttcagtaac aaaatagtca tttcctccta tcctggtagt accccctata
     2641 tactccaatt caccacctat atgtactgta ggatcaagtc catattccaa cattaccata
     2701 gctatcatgg aggttgtagt tgtttttccg tgagttcccg atactgcaat actatgagga
     2761 tattttttca ttaaaagtcc aagaagtgtt gccctgtcga ttataggtat atctaaagac
     2821 cgggctttta caagctcagg attattatcc tttattgcgg cagtatagat tacagcatca
     2881 ggattgttaa tatttccttt atcatgatta taataaatta ctgcaccttt gctttccagt
     2941 ttttgtgtta tatttgaa
//
LOCUS       DAOSDD010000393         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_11807_length_2953_cov_5.914424, whole genome shotgun sequence.
ACCESSION   DAOSDD010000393 DAOSDD010000000
VERSION     DAOSDD010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11807_length_2953_cov_5.914424"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK733_19480"
     CDS             <1..860
                     /locus_tag="PK733_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOJ12753.1"
                     /translation="LNFRLTHKKGEKNESTRQLKSSVIIRVIVVITYVGMVSVNALAN
                     LLPINGINTGQVSDSYPNLFAPAGLTFAIWGLIYLLLAGYSVYQLGVFQKNKKNIKSN
                     LLNKVGILLSISSIANMIWIFSWHYLVIPLSMLLMLVILICLILIMKLINNEESLTSA
                     EKTFMRMPFSIYFGWITVATIANATTLLVSIHWDGFGITEQTWTVLILIIGVLIGALT
                     TIKNRDIAYGLVIIWAYTGILIKHLSKDGFAFQYPSVVNTVVSCIVLLIITLAYVLIK
                     AKQIKNIAK"
     gene            939..1190
                     /locus_tag="PK733_19485"
     CDS             939..1190
                     /locus_tag="PK733_19485"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ12754.1"
                     /translation="MKYDVVIIGGGISGLTTGSLLAKRGLHVVVIDKNYNPGGSCGIF
                     KRNDVVFDQGSAMLFGFGEQGFNTHRFVFNCLEEPINII"
     gene            1280..1786
                     /locus_tag="PK733_19490"
     CDS             1280..1786
                     /locus_tag="PK733_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12755.1"
                     /translation="MFPSEKNNIKRFYHELQKMYRHIMVENPTYTTPDETDLRGSLKS
                     LLKHPVSYSRFLSYLNKSAESLLKKYFTDPEIFKFFDKLTSTYCYTTVKESPAVLASV
                     MFVDNHVGGSYYPAGSTLFLPGKMEKVIEENGGDMMYQSEVTGIIFDQSRAIGVDLTQ
                     SYRFSCTF"
     gene            complement(2010..>2953)
                     /locus_tag="PK733_19495"
     CDS             complement(2010..>2953)
                     /locus_tag="PK733_19495"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12756.1"
                     /translation="LLKRYKDKYHCSIYSYILMDTHVHIYINTCGADISSFMRSLNIA
                     YVRYYNKKYQRHGHLFGGRFASFIVDNNTYSLTLSAYINNNAKDLPGYSGKEELYPYS
                     SYGIYTGYRDELDGIVDTDFILKCFSSDKKVAREKYRAFTESMKNKDIINEVDGNITG
                     AYTENKYKSEKSYIVRSENPDEVLGKIEKLLGEKLSEGFSEELPGELQAELAVGFSER
                     PSVGLPTSLSRELHVGLSLGISTDLRLKHNREVVKIRAFATYILRTLCGYTYKQLCQY
                     IGNMSLSGISRLVNEGFKLLKEHVVFRDVFNAIIKTG"
BASE COUNT          907 a          496 c          533 g         1017 t
ORIGIN      
        1 ggcttaattt tcgcctaact cacaagaaag gagaaaagaa tgagtctact cgacagttaa
       61 aatcaagtgt aattattaga gtgatagttg ttattaccta tgttgggatg gtcagtgtga
      121 atgccctggc aaacttactt cctatcaatg gtatcaatac cgggcaggtg tcggattcgt
      181 atccaaactt atttgcgcct gcagggctta cttttgcaat atggggactg atttaccttc
      241 ttttagctgg ttattctgta tatcaacttg gtgtatttca aaaaaataaa aaaaatatta
      301 aaagtaactt actgaacaaa gtcggtatat tgttatctat atcatctata gctaatatga
      361 tttggatatt ctcctggcac tatcttgtca tacctctatc catgttgctt atgttagtta
      421 tattaatttg tcttattttg attatgaaac tgattaataa tgaggaaagt cttacatctg
      481 ctgaaaaaac atttatgcgg atgccattta gcatttattt tggatggatt actgtcgcta
      541 ctatagcaaa tgccactaca cttcttgtaa gtattcattg ggacggcttc ggaatcacag
      601 aacaaacgtg gacagttttg attcttatta ttggggtatt aattggggct ttaactacaa
      661 tcaaaaacag agatattgca tatgggcttg ttatcatatg ggcttataca ggtatattga
      721 taaaacattt atcaaaggat ggctttgcat ttcagtatcc ttcggtagtt aataccgtgg
      781 tatcgtgtat agtccttctt attatcactt tggcatatgt tctgataaaa gcaaagcaaa
      841 taaaaaatat tgcaaaatag ataaataaat actttatata ctaaatggtg tactaaatag
      901 cctgctaaat aatctactag atggtggtga gagttatcat gaaatatgat gtagttataa
      961 tcggaggtgg gatatcaggc ttaacaactg ggagtttact tgctaaaaga ggtcttcatg
     1021 tagtggttat agataaaaac tataatccgg gtggttcctg cgggattttt aaacgaaatg
     1081 atgtggtgtt tgatcaggga tctgccatgc tttttgggtt tggagagcaa ggctttaata
     1141 ctcatcgttt tgtgtttaat tgtcttgaag aacctataaa tataatctag catgatcttc
     1201 tctgtattgt gttaacttta aaggctaccg cataaaattt tggccggatg ttgacatgtt
     1261 tgcagaggag ttatccaggg tgttcccatc tgaaaagaat aacataaaga gattttatca
     1321 tgagcttcag aaaatgtatc ggcatattat ggtggaaaat ccaacctata ctactcccga
     1381 tgaaacagac ctcaggggtt ctttaaaaag tctgctgaag caccctgttt cttattcaag
     1441 gttcttaagt tatctaaata aaagtgcaga aagcctgcta aaaaaatatt ttacagatcc
     1501 agagattttc aaattttttg ataagcttac atccacatat tgctatacca cagtgaaaga
     1561 gtctcctgct gtattggcct ctgtgatgtt tgtggataat catgttggtg gaagctatta
     1621 tccggcaggg tccacgcttt ttttacctgg caagatggaa aaagtgattg aagaaaatgg
     1681 cggagatatg atgtatcaaa gtgaagtgac tggtattata tttgatcaaa gtagagccat
     1741 tggggtagat cttacacagt cttataggtt ttcgtgcact ttttgaaatt ttccataata
     1801 tagagaattt tatatagagt attttattgt agataattct agttcagata aaaaaataga
     1861 atagcaaaag gaccttaaca ctttgtgcaa ggtctgagaa taaagagcct gacatttaag
     1921 cctctttaat attagttata tttagtatat ttattttatt ggggagtcaa aaatttgaaa
     1981 aattaatcag attaataatt aaattaatat taacctgttt taattatggc attaaagaca
     2041 tccctgaaca ctacatgctc tttcagcagc ttgaaacctt cattaacaag ccttgagatc
     2101 cctgacaatg acatgtttcc tatgtattgg cataattgtt tgtatgtata tccacagagt
     2161 gtcctcagaa tatatgttgc aaatgccctg attttgacga cttccctgtt atgctttaac
     2221 ctcaaatctg ttgatattcc taaggataat cctacatgca actctcttga taagcttgtc
     2281 ggtaatccta ccgatggtct ttccgaaaat cctaccgcta attctgcttg taattcaccc
     2341 ggtaattcct cagaaaatcc ttctgaaagc ttttcaccaa gcaatttttc aattttgccc
     2401 agtacctcat cgggattttc actccttaca atatagctct tctcactctt atacttgttt
     2461 tcagtataag ccccggtaat attaccatct acttcgttta taatgtcttt attcttcata
     2521 gattcagtga aagccctata cttttcccga gccacctttt tgtcgctgct aaagcacttt
     2581 aatataaaat ctgtgtctac aattccatca agctcatctc tgtagccggt atatattccg
     2641 tatgatgaat aaggatacaa ttcttctttt ccactataac ccggaagatc tttcgcattg
     2701 ttattgatat atgcagatag tgtcaagctg tatgtattat tatcaactat gaagctggca
     2761 aatctgcctc caaacaaatg cccatgccgc tggtatttct tattataata tctgacatag
     2821 gctatattaa ggctgcgcat gaaagacgaa atatctgcac cgcacgtatt aatgtatata
     2881 tgaacatgag tatccataag gatatatgag taaatactgc aatgatattt atctttatac
     2941 cgctttaaga gtt
//
LOCUS       DAOSDD010000394         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12501_length_2826_cov_4.876218, whole genome shotgun sequence.
ACCESSION   DAOSDD010000394 DAOSDD010000000
VERSION     DAOSDD010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12501_length_2826_cov_4.876218"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1347)
                     /locus_tag="PK733_19500"
     CDS             complement(256..1347)
                     /locus_tag="PK733_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12757.1"
                     /translation="MGNNLKIGVVGLRRGRSLLGDMLLMPEVTVSAICDKDEERLKSC
                     EDWLKQEQKRDGFVSLNNFEDLLNTDVDVVIIATEVSAHVSMSIQALEAGKHVFSEVP
                     AINSLDEARKLYQAVKKSGRKYMLGENCCFWAFINTWKEIYESGRLGDVWYSEAEYLH
                     NVIELMKDKNGNPTWRATYDAIKYLTHDLGPLLYITDDGCVSVSGFAPEINPIPEYST
                     GTPNEVAIFKTEKGALIKIFVSFGLQREPARHNFCIYGSKGTLETVRSGEHSTIAYFK
                     DIPHTTDLIKIPVGVSYPGASTKILGGHGGADYYMMKSFVKSVIEDTKPPIDVDLAIR
                     ISLPGIYAHMSTLQGGVPIKIPNVRELCE"
     gene            complement(1594..>2826)
                     /locus_tag="PK733_19505"
     CDS             complement(1594..>2826)
                     /locus_tag="PK733_19505"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOJ12758.1"
                     /translation="RSSFAFVISTVLAFIFFSLHSNGISLFMLSENYSIMYFMFIAAM
                     CIAGYFGLVILSKLKVQARQGSEGEVKMLSSLYKVICFFALLMGIMFVFGKMESFGAF
                     FSMFGGMLLGWSLQAPVSGFAAWIMVIIMRPYKLGDRIQFPSLGLIGDVVKFSPMYLT
                     LNQVGGTIGSEEPVGRMVHVPNAMLFGQVAINYTYQQKKEAGAYILDEAVFRVTLDSD
                     WDAVEKILLNTAREVTKDIIQEIGAEPYVRADTWDYGTLFRLRYMTDATDRPRIMYEI
                     VKKATKEIQRNKNVDLAIPFIYSFKRGLDGMGTAPKQNEAIEEIDISNIICEKLHDDN
                     FLKENEEEIYEIAKNINEVGLLQPIIVQRNIDNGKYTLLYGEKRLKACMLLGWEKIPV
                     IIGNKYGTEFINKNMSQR"
BASE COUNT          858 a          592 c          410 g          966 t
ORIGIN      
        1 caatttattt ttttataatc ctgtgcaaaa aatatgcaga tgtgcattag cttttcagaa
       61 atttatcaat ttcattcaaa ctatcctcca ggttatttta ttactaattt tattttatac
      121 tttattttca attttattta tgttctgttt tctatacata taatatatca ttcgcacatt
      181 ttattttcaa tatcttaaat taatagttcc tctctttaga taaatcacag cttaaagaga
      241 ggaactatcg attgcttact cacataattc cctgacattg ggtattttta taggaacccc
      301 tccctgcagc gtagacatat gagcatatat tcccggcaaa gatattctta tagccaggtc
      361 aacgtctata ggaggtttgg tatcttctat tactgatttt acaaagcttt tcatcatata
      421 ataatcagct cctccatgtc ctcctagaat tttagtggat gcgccaggat aagaaacacc
      481 tacaggtatc ttaataagat ctgtagtatg aggtatatct ttgaaatatg ctattgtaga
      541 gtgttctcca cttctcacag tttcaagggt acctttgctt ccatatatgc aaaaattatg
      601 acgtgcaggt tctctttgca agccaaagga cacaaatatt ttaattaatg cacccttttc
      661 tgttttaaat atagccactt cattaggtgt acctgtggaa tattccggaa ttgggttgat
      721 ttcgggtgca aatccgctta ccgaaacaca cccgtcatct gttatatata acaaaggccc
      781 cagatcatga gtgaggtatt ttattgcatc ataagtagct ctccaggtag ggttgccgtt
      841 tttatccttc attagttcaa ttacattatg taagtattct gcttctgaat accacacatc
      901 tcctaaacgt ccggactcat atatctcttt ccatgtgttt atgaatgccc aaaaacaaca
      961 gttttctcca agcatatact tgcggccgct cttctttacg gcctgataaa gcttcctggc
     1021 ttcatcaaga gaattgatag caggaacttc tgagaataca tgttttcctg cttcaagggc
     1081 ttgaattgac atagacacat gagcagatac ttccgtagct attattacta catcgacatc
     1141 tgtatttaga aggtcctcaa aattattgag gcttacaaat ccatctcgtt tttgttcttg
     1201 tttcagccaa tcttcacatg actttaacct ttcttcatct ttatcgcata tagctgaaac
     1261 agtcacctca ggcattaaaa gcatgtcgcc caataaactg cgcccacgac gcagaccaac
     1321 aacaccaatc tttaaattat ttcccatgat gaacaatcct ccatataaaa taagctgtgc
     1381 tataaatttt aatatgtctt tgcagactct tattattata agtctaataa aggatagaaa
     1441 caatattata atgtcataat atcgtaatgc cataatttcg tatcaggtac atttataaaa
     1501 tttagagaaa tacttaccag aggctgcttt tacgttaaat agagcctttt aatggtatga
     1561 tggcaaaatc aaacttgcaa aacaacttat attttacctc tgcgacatat ttttattaat
     1621 aaattccgtc ccatacttat ttccaataat tacgggtatc ttctcccatc ctaatagcat
     1681 acaggcttta agacgctttt caccatataa aagtgtatat ttgccgttat caatattcct
     1741 ttgaacaata atgggctgaa gcaatccaac ttcatttatg tttttagcaa tttcatatat
     1801 ttcctcttca ttttctttta gaaaattatc atcatgtaac ttttcgcaaa taatattcga
     1861 gatatcaatt tcctcgattg cttcattttg ttttggagct gtgcccattc catcaagtcc
     1921 tcttttaaat gagtaaataa atggtatcgc caaatctaca tttttattac gctgaatctc
     1981 ctttgttgct ttctttacaa tctcatacat aatccttggt ctatctgtag catctgtcat
     2041 gtatctcaac ctgaacaatg ttccataatc ccatgtatca gcccgtacat acggctctgc
     2101 accaatttcc tgtataatat cctttgttac ttctctggca gtattaagaa gtattttttc
     2161 aacagcgtcc caatcagaat caagagttac tctgaacact gcttcatcca gtatataagc
     2221 accagcttct ttcttttgct ggtatgtata gtttatcgct acctgtccga aaagcatggc
     2281 attgggtaca tggaccatcc ttcctactgg ttcctcgctg cctattgtac ctcctacttg
     2341 atttaatgta agatacatgg gactgaattt aaccacatca cctatgagtc caagagaggg
     2401 aaattgaatt ctatctccca gtttatatgg ccgcataata ataaccatta tccatgcagc
     2461 aaaaccgctt acaggagcct gtaatgacca tccgaggagc atgcctccaa acatggagaa
     2521 aaatgctcca aatgactcca tttttccaaa tacgaacatt attcccatta gcagtgcaaa
     2581 gaagcatata actttatata ggctgctcag catttttacc tcgccttcac ttccctgcct
     2641 tgcctgaact tttagcttgg aaagtatgac cagtccaaaa tatcctgcaa tacacatggc
     2701 ggcaataaac ataaaataca ttattgaata attttctgaa agcataaaaa gtgatatgcc
     2761 attactatga agtgaaaaga agataaaagc caatactgta gaaattacaa aagcaaaact
     2821 acttct
//
LOCUS       DAOSDD010000395         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12797_length_2775_cov_5.122059, whole genome shotgun sequence.
ACCESSION   DAOSDD010000395 DAOSDD010000000
VERSION     DAOSDD010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12797_length_2775_cov_5.122059"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(556..632)
                     /locus_tag="PK733_19510"
     tRNA            complement(556..632)
                     /locus_tag="PK733_19510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Arg,seq:acg)
     gene            complement(929..1702)
                     /locus_tag="PK733_19515"
     CDS             complement(929..1702)
                     /locus_tag="PK733_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOJ12759.1"
                     /translation="MSTYRRYRKRKLNKAKLFKNLLFMFILISVVFISGRYIIKITYA
                     VLTSTEEMNKAEDILILVNKEHDIPEKYVPGNLVEADVQFPTTTAMDQKMMQKDAAKA
                     LEKLFKSAEKESINLFGVSGYRSFDYQKKIYDKKVKAVGREEAGKYVAYPGQSEHQTG
                     LAMDVTNKAGASNILTENFGKTKEGQWLKDNAHYFGFIIRYPKGKEDITGYNYEPWHL
                     RYVGIEAATEIKSRGIVLEEYLQNNDSTEDAENAGAAGD"
     gene            complement(1820..2605)
                     /locus_tag="PK733_19520"
     CDS             complement(1820..2605)
                     /locus_tag="PK733_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ12760.1"
                     /translation="MEKDNSSTQQTFITYNDILNGLKRLNLPENPILLVHSSMKSFGY
                     VEGGPETVIKALIDTCGNKGTLVMPTLTFASINEAEPFFDASETPSGTGLITEVFRKM
                     PDARRSLHLFSSAAALGEKAEYITSYHDDTPCGPGTPYQKIIELEGYVLFIGAGFGSN
                     TLFHVAEEYVNPPYMRYKTIKNAKINDLEGNTYFRDIRRYDCYQTGIIRRLDRMKDIF
                     ENENVLTRTVIGNSNITLIRAEDNFRISCNLLGNNYRYILENE"
BASE COUNT          827 a          555 c          422 g          971 t
ORIGIN      
        1 ggtgtaggtt ctgtaaaaaa gaactgatag accaggtacc tatggggtac aatcctgcat
       61 aggagggtag agtggcacta cctaaggttc cattgatgaa ggtgggtcgg aaacggaaca
      121 actggagttc atttctcttc cacttgagat aggaaccacc gaacttccac atggaagcgc
      181 ctcaatttat tgaggtgagg atgtcacagc tacgcatcat tccaatacta gtactaatca
      241 ttgatattaa gtaggagtga tcattgcgtt ctcatatatt gtaaaattgg cagtacttaa
      301 aatccgccac caaaccttat atactgctgg attaattgag tatattatat tatggcatgt
      361 tatttcgaca agcatcaccc atacgcataa tacctatttt tttaattttt attgattgtt
      421 cttcgcacct atctaatatt tttgttagcc aatgtaagcc aataaacatc tcaagatcct
      481 gaccttttga ctctataagt catacaaaaa atcagagggt atatactgta atgtatatac
      541 cctctaatcc ctctgtggcg tgccattcag gacttgaacc cgaagccttt cggtccgtag
      601 ccgaacgcta tatccaattt agctaatggc acatatttaa cttttcccac taaaactacg
      661 tttacctttt cctccgtagt caagcgctct atccaattga gatatgggcg catattaata
      721 ttccatattt aatagttcaa tttaagttca agcattcact taaattaaat atcatattaa
      781 taataataca aatccattat aaaatcaact atattttttt gctaatttcg ccatggtatt
      841 acataagagt cattttcctg aggaatatct ttctaatgct attaaaagtt atcctggaag
      901 acattaatct tttaatgata aggtggcatt aatctcctgc agctcctgca ttttccgcgt
      961 cttccgtact atcattattt tgcagatact cttccagaac tatccccctt gatttaatct
     1021 ctgtggccgc ttcaatccct acatatctta aatgccatgg ctcatagtta tatccggtta
     1081 tgtcttcttt acctttagga tatctaatta taaacccgaa ataatgtgca ttatctttca
     1141 gccattgtcc ttcttttgtt ttaccaaagt tttctgtcag gatatttgaa gcacctgcct
     1201 tatttgttac atccattgcc aggcctgttt gatgctcact ttgtccagga taagctacat
     1261 atttaccggc ttcttctctt cctacagcct taactttttt atcatatatc ttcttttgat
     1321 aatcaaaaga cctgtatccg gacacaccaa acagatttat actctctttt tccgcactct
     1381 taaataactt ctcaagtgct ttagcagcat ctttctgcat cattttttga tccatagcag
     1441 tggtggtagg aaattgaaca tctgcttcaa cgagattacc aggtacatat ttttccggta
     1501 tatcgtgttc cttattcacc aaaatcaaaa tatcttcagc tttattcatt tcctcagtag
     1561 atgttaatac tgcatacgtg attttaataa tatatctccc tgatataaat accactgata
     1621 ttaatataaa catgaaaagc aagtttttga aaagctttgc cttattaagt tttctcttcc
     1681 tgtatcttct gtaagttgac ataatacccc cgctctcctg ttacaataat aaacaagttt
     1741 tgtaacaggt atattacaac taaataaaat attcaggaca atttatgata aataacatta
     1801 tctgctaata agaaatgatt tactcatttt caaggatata cctataatta tttcctagca
     1861 aattacagct tattctaaaa ttatcctctg cacgtataag tgtaatattt gaattaccta
     1921 tcacagtcct ggtgaggaca ttttcatttt cgaaaatatc cttcatcctg tcaagccttc
     1981 tgattatacc tgtctgatag cagtcataac gtctaatatc cctgaagtat gtattacctt
     2041 ccaggtcatt aatttttgca ttctttatag ttttataccg catgtaggga gggtttacat
     2101 actcctcggc tacatgaaaa agagtattag atccaaatcc tgcacctata aatagaacat
     2161 agccttccag ttcaattatc ttctggtatg gtgtacctgg tccgcaaggg gtatcatcat
     2221 gatatgaagt aatgtattct gccttttccc ccaaagcagc cgcagatgaa aaaagatgaa
     2281 ggcttcttct tgcatccggc attttacgga agacttcagt tattaatcct gttcccgagg
     2341 gtgtttcaga ggcatcaaag aatggttctg cctcatttat gctcgcaaag gtgagagttg
     2401 gcataactag agttccctta ttcccacaag tatcaataag tgctttgatc actgtctcag
     2461 gtccaccttc cacataccca aaagacttca tggaactgtg aacaagcaga ataggattct
     2521 caggaaggtt aagtcttttt aatccattaa gtatatcatt atatgttata aacgtttgtt
     2581 gagttgaaga attatctttt tccatcaggt tatccccttt atagctttat aataacaatt
     2641 tataattaca tatttataat taaattataa agctattata aagcaaattg aattttttgt
     2701 acatccggat tttttattgc gggtgcattt aaaatttttg aagaaatata ttccagaagc
     2761 tatcgttacg gtact
//
LOCUS       DAOSDD010000396         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12798_length_2775_cov_4.633456, whole genome shotgun sequence.
ACCESSION   DAOSDD010000396 DAOSDD010000000
VERSION     DAOSDD010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12798_length_2775_cov_4.633456"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK733_19525"
     CDS             <1..794
                     /locus_tag="PK733_19525"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ12761.1"
                     /translation="VVSETFFQWVIEDNFKNKPYIQKQQLYSWGIENFTWNDWAPVVA
                     RYYAIISQLDDAIGRIINKLDSLGLADDTLVIYTADHGDMCGSHRMMDKHYVLYDDIV
                     KVPLIARWPGVVKSGAVCSNFICHSLDIPPTMLDIVHIENKEFFQGNSLLPLLKGDGK
                     GNSTNNLRQYAVSTYNGQQFGLYTQRMIRTEKWKYIWNTTDIDELYDLQEDPYELHNV
                     IHETQYSGMVAELRYLLYEELLKDKDGLVLGNEWLKKQLIGGKKY"
     gene            938..1363
                     /locus_tag="PK733_19530"
     CDS             938..1363
                     /locus_tag="PK733_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ12762.1"
                     /translation="MDKTKVIGNLKGNMAFQMNLDGHTLTTDASESIGGNDLGPRPKQ
                     LLLAGLIGCTGIDVVLILKKMKVEFEDLSIEVEAENTEELPKVYKNIHLTYRFKGKNL
                     SRNNIERAVSLSQEKYCGVSAMLKKAAPITYDIIIEENS"
     gene            1381..2319
                     /locus_tag="PK733_19535"
     CDS             1381..2319
                     /locus_tag="PK733_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12763.1"
                     /translation="MENDKLIEIKNNLSTLQVLEKRMDRLIKSIYEAEAEVQSLLVKY
                     EAESLDVDKLKDDSFSTTLLRLFGRYDGKLDKETEEMLSAKMAYDKAVERVKDLKQER
                     ENLGSRISAMRNEIRLYEDEIKRREERIISNNDSEVSTKYMELENERKMMIQQLVETD
                     EALKAAIGVTNTGERAAQHLGSAEKWATYDVWFKSGIISHMAKYDHIDNAESEFNRLD
                     SQLKNLKKELKDVRMLDTPEISTISSSQRAVDFWFDNIFTDLNVRGKIRNDIEQLESL
                     LSSIYKIISRLESNKTNINRKLKSIDEQKNNLLINL"
     gene            complement(2426..>2775)
                     /locus_tag="PK733_19540"
     CDS             complement(2426..>2775)
                     /locus_tag="PK733_19540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12764.1"
                     /translation="PQTVYTKTHIISFAIEYINQNYNNNITIADISRFCCCSQSYISH
                     NFKERLGMSVPAYISKVRIERAKTLLKSTNLPINQISAEVGYNDPNYFSTVFTQHCGM
                     SPRDYRKSCETSD"
BASE COUNT         1005 a          383 c          593 g          794 t
ORIGIN      
        1 ctgttgtctc tgaaactttt ttccaatggg tcattgaaga caatttcaaa aacaaacctt
       61 atatacaaaa acaacagcta tacagttggg gcatagaaaa ttttacctgg aatgattggg
      121 cgcctgttgt tgcccgatat tatgcgataa tcagccagtt ggatgatgca ataggcagaa
      181 ttattaataa acttgattca cttggactgg cagatgatac acttgtgata tatactgctg
      241 atcatggaga tatgtgcgga tcccacagga tgatggataa acattatgtg ctttatgatg
      301 atatagttaa ggtaccactg attgcaaggt ggccgggagt tgtgaaaagc ggtgccgtat
      361 gcagtaattt tatttgccat tcccttgata tacccccaac aatgcttgat atagttcata
      421 ttgaaaacaa agagttcttt cagggaaatt cactgctgcc gcttctaaaa ggtgatggta
      481 aaggtaatag tactaataat ctgagacaat atgcagtatc aacctataat gggcagcaat
      541 ttggattgta tacacagagg atgatcagaa cggaaaagtg gaaatatata tggaatacaa
      601 cagatataga tgaattatat gacctgcagg aagaccctta tgaattgcat aatgttatac
      661 atgaaactca gtacagtggg atggtagcag aattgcggta tttgctgtat gaggaattat
      721 taaaagataa ggatgggttg gtactgggaa atgaatggct taagaagcag ttaattggag
      781 ggaaaaaata ctaaagttca aaatattata gtaaaatatt attgtaatat tagaggataa
      841 taattttcta ttcactaaga gagtagatca tttataaaat agtcgtgaac actcatgact
      901 ttagtagtga gagatttcaa gaaaggggaa taataatatg gataagacaa aggtaatagg
      961 aaatttaaaa gggaacatgg ctttccagat gaatcttgat gggcatactc taacaactga
     1021 tgcttcagaa agcattggcg gaaatgacct tggaccgcgt ccaaaacagc ttcttcttgc
     1081 tggattgatt ggctgtacag gtattgatgt agtattaata ttaaagaaaa tgaaagttga
     1141 atttgaggat ttaagcattg aagttgaagc agaaaatacg gaagagcttc ccaaagtata
     1201 taaaaatatt catttaacct acagatttaa agggaaaaat ctctccagaa acaacataga
     1261 aagggcagta tcattatccc aggaaaaata ttgtggtgtc agtgccatgc tgaagaaagc
     1321 agcaccgatt acttatgaca ttataatcga agaaaatagt tgatgcagga gtgaatataa
     1381 atggaaaatg ataaattgat tgaaattaaa aacaatcttt caaccctcca agttttagag
     1441 aaacgcatgg acagactgat aaaaagtata tatgaagcag aagccgaagt acaaagtcta
     1501 ttagtaaaat atgaagcgga atctttggat gttgataagt taaaagacga tagtttttca
     1561 acaacacttt tgagattatt tggcagatat gatggcaagc ttgacaagga aacagaggaa
     1621 atgctgtctg caaagatggc atatgataag gcggttgaaa gggttaaaga tctaaagcag
     1681 gaacgtgaga atcttggcag tagaatatct gctatgagaa acgaaataag gctttatgaa
     1741 gatgagatta aaagacggga agaaagaata atcagcaaca atgattctga agtatcgact
     1801 aaatatatgg aattggaaaa tgaaagaaaa atgatgatac aacagctggt agaaaccgat
     1861 gaagcattaa aagctgctat tggagtaaca aatactggcg agagagctgc tcaacatctg
     1921 ggaagtgctg aaaaatgggc cacctatgat gtttggttta agagcggaat aataagccat
     1981 atggcaaagt atgatcatat tgataatgct gaatcagagt tcaatagatt ggattcacaa
     2041 cttaaaaatt taaagaagga acttaaggat gtcagaatgc tggatacacc ggaaatttct
     2101 actatcagct catcccaaag agccgtagat ttttggtttg ataatatttt caccgaccta
     2161 aatgtgcggg gcaaaatacg taatgatata gagcagcttg agagcctgct gagttctata
     2221 tataaaatta tatcccggct tgagagtaat aaaacaaata tcaatagaaa acttaagagt
     2281 atagacgagc aaaaaaacaa cctgcttatt aatctttgag aaatatgaac agaaacatgg
     2341 gatggttctt ttgtgccgtt ttttaacaat ctaggtaata aaacgtaaga accatcatca
     2401 tgttcctgct gtttcgtctg aaggctcaat ctgatgtttc acaggatttc ctataatctc
     2461 taggggacat tccacagtgt tgtgtgaaaa cagttgaaaa gtaattagga tcattatagc
     2521 ctacttctgc tgaaatctga ttaattggta aatttgtgct ttttagcaaa gtttttgcac
     2581 gctcaatacg aactttacta atatatgcgg ggacactcat tcccaatctt tctttaaaat
     2641 tatgacttat atatgattgg ctgcagcagc aaaaccttga aatatctgca atagtgatat
     2701 tattgttata gttttggttt atatattcga tagcaaaaga tatgatgtga gtttttgtat
     2761 agactgtttg aggag
//
LOCUS       DAOSDD010000397         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12824_length_2770_cov_5.014365, whole genome shotgun sequence.
ACCESSION   DAOSDD010000397 DAOSDD010000000
VERSION     DAOSDD010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_2770_cov_5.014365"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..434)
                     /locus_tag="PK733_19545"
     CDS             complement(210..434)
                     /locus_tag="PK733_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12765.1"
                     /translation="MARRSISIDEKIKKQKEVVATAKVKYDAAVNELNILIKKKQELQ
                     GKELLNAFVNSDKSLDEILAFMSGKKDDEA"
     gene            complement(409..2154)
                     /locus_tag="PK733_19550"
     CDS             complement(409..2154)
                     /locus_tag="PK733_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12766.1"
                     /translation="MYANVRAKIPEEKGKVTRKKINGTTYIYYQIDRVYNPEKKYSIP
                     KSTPIGKICEDDPSMMIPNERYLTFFPEADLPDVKKSAYRSACLRVGTYMVLRRIIAQ
                     YHLDELMSELIGKDSGLFLDIAVYTIIAENNAGQYYPDYAYNHPLFTDKMKMYSDSKV
                     SDFIGSIKKSQSVEFLNKWNKNRDHREKIYISYDSTNKNCQAGDVDFVEIGHAKDDQN
                     KPVLNYSIAYDRNNREPLFYEMYPGSIVDVSQLQYMLEKTSGYGYRRVGFILDRGYFS
                     KENIRYMDKCGYDFVIMMKGMKKYASDLVMKNKGSFEESRSHSIRYYKVSGTTVKGKL
                     FPSDEKDRYFHIYYNERKRTAEREQLEEKIDRMATYLANQEGKTQYECPSAFCHYFDP
                     IYHVQGDEKTFMFARERQDVIDSEIKLCGYFIIITSEKMSAEEALDLYKSRDGSEKLF
                     RGDKSYLGNKSFRVHGSESVNNKIFIEFVALIIRNRFYTYLKEQMRKSEKKENFMTVP
                     AALRELEKIEMIRQTDGNYRLDHAVTATQKEILKAFDMTERNIREQAIGINQKLQMID
                     QIKGDNDNGQTKYID"
     gene            complement(2377..>2770)
                     /locus_tag="PK733_19555"
     CDS             complement(2377..>2770)
                     /locus_tag="PK733_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOJ12767.1"
                     /translation="INKLPLIHKCLQVAFTFCLISFAWIFFRANSIKDAFYIVTHLFT
                     GIGKITDVKYLVNSISNMALTKFQLIISVAGIIIIELVHLFQRKGSVFDRISEKPALI
                     RWAAYSILIITTFWLAFSENKQFIYFQF"
BASE COUNT          765 a          579 c          415 g         1011 t
ORIGIN      
        1 aattagtgtt attagtatta ccttgaaaat aaaccctttt aatctcattt aaccccaact
       61 tccgtccttt cgtatatctt atacatttcg cttatcttat atataattta catattaaat
      121 ataacgcaca aaaaatctat attatttcgg ttgcttaata ctactttgtt tcttaacttc
      181 ctgaaataat taactctcta gtgcatattt tatgcttcat catccttttt cccactcatg
      241 aaagcaagaa tttcatccaa acttttatca ctgtttacaa atgcattcag caattcttta
      301 ccctgtaatt cctgtttctt ttttatcaaa atattgagtt catttactgc agcatcatat
      361 ttgactttag ctgttgcaac cacttctttt tgtttcttta tcttttcatc aatcgatata
      421 cttcgtctgg ccattatcat tgtctccttt tatttgatct atcatctgca gtttctgatt
      481 gattcctatc gcctgttccc ggatatttcg ctctgtcata tcaaacgcct ttaatatctc
      541 cttctgggtg gctgtcaccg catgatcaag gcgatagttt ccatcagtct gcctaatcat
      601 ctctatcttt tcaagttctc tgagtgctgc tggaacagtc atgaaattct ctttcttttc
      661 actcttgcgc atctgctctt tcagatatgt gtagaaccta tttcgtataa tcaacgctac
      721 aaattctata aaaatcttat tattgactga ttcacttcca tgcactcgga agctcttgtt
      781 tcccagatat gatttatcgc ctctgaagag tttctccgaa ccatcacggc tcttatatag
      841 atccagcgct tcttctgctg acatcttttc cgaagtaata ataatgaaat atccgcacag
      901 tttgatttca ctgtcaatca catcttgcct ctctcttgca aacataaagg ttttttcatc
      961 cccttgtacg tgatagattg gatcaaagta gtggcagaat gcgcttggac attcgtactg
     1021 tgtttttcct tcctgatttg ccaagtaagt tgccatacgg tcaattttct cttcaagctg
     1081 ttcccgttcc gctgtgcgtt tacgctcgtt ataataaata tgaaaatatc tgtctttttc
     1141 gtcagaagga aacaattttc ctttcaccgt ggttccgcta actttataat atcggatact
     1201 gtggcttcgg ctttcttcga agcttccttt gtttttcatt acaagatcgc tggcatactt
     1261 cttcatgccc ttcatcatga tgacaaaatc atagccacat ttatccatat atcgtatatt
     1321 ttctttgctg aagtatcctc tgtctaagat aaaaccgaca cgtcggtaac catatcctga
     1381 tgttttttcc agcatatact gcagctgtga tacatctact atgctaccag gatacatctc
     1441 gtaaaacaaa ggttctctgt tatttctgtc gtaggctata gaatagttca ataccggttt
     1501 gttttggtca tccttggcat ggcctatttc cacaaaatca acatctccag cctggcaatt
     1561 cttattggtt gaatcatacg atatgtagat tttttctctg tgatctctgt ttttattcca
     1621 tttatttaga aattctacac tctgactttt cttgattgaa ccgataaaat ctgaaacctt
     1681 ggaatcactg tacatcttca tcttatcggt aaacaacgga tgattgtagg catagtcagg
     1741 ataatactga ccagcattat tttctgcaat aatggtatag acagcaatat ccaaaaagag
     1801 tccactatct ttaccaataa gttctgacat cagctcatcc agatggtact gtgcaataat
     1861 tcttcttagt accatatatg tcccgactct cagacaagca cttctgtaag cactcttctt
     1921 cacatccggg aggtctgctt caggaaaaaa agtcaagtat ctctcgttag gaatcatcat
     1981 ggaaggatca tcttcacaga tctttccaat tggtgtactt ttgggaatgc tgtatttttt
     2041 ttcagggttg taaactctat caatttgata gtaaatgtag gtggtcccgt taattttttt
     2101 ccgggtaact ttgccttttt cttctggtat tttagctctc acattagcgt acattatggc
     2161 ctcctttaga gagttaatta actctctatt atcgtaccat attttgctga acaatgcaat
     2221 gaaaatcggg tatttttgct gaaaaatggc actttctaaa atttctacat tcgtagagaa
     2281 taaccttgag agttaattat tgtggaagtc cacattgtta tggccaatgg aaagcgcttt
     2341 ccaaattggc atagcattgc taatttggta tagcacctaa aactggaaat aaataaattg
     2401 cttgttttca ctaaatgcca gccagaacgt tgtaattatt aatatagaat aagctgccca
     2461 ccttattaag gcaggttttt cagaaatcct gtcaaaaacg ctccccttcc tttgaaaaag
     2521 atgcaccaac tctattataa taattcctgc aactgaaatt atcaactgga attttgtaag
     2581 tgccatattt gatatggaat ttaccagata ttttacatca gttattttcc caatgccagt
     2641 aaacagatgt gttactatat aaaaagcatc ttttatggaa tttgccctga aaaatatcca
     2701 tgcaaaggat ataagacaaa atgtaaatgc aacctgcaag catttatgta ttaagggcaa
     2761 cttattaatc
//
LOCUS       DAOSDD010000398         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12847_length_2766_cov_3.362228, whole genome shotgun sequence.
ACCESSION   DAOSDD010000398 DAOSDD010000000
VERSION     DAOSDD010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12847_length_2766_cov_3.362228"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..2598
                     /gene="fusA"
                     /locus_tag="PK733_19560"
     CDS             508..2598
                     /gene="fusA"
                     /locus_tag="PK733_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ12768.1"
                     /translation="MKDYTVEKLRNVCLLGHGGSGKTTLAEAMLFNTGVLDRFGKVPD
                     GTTTTDFDPEEIKRKISINTAIAPCEWNGHKINVIDTPGFFDFIGELKQGLKVAESAV
                     ILVPGKGGVAVGTEKSWQYANEHGMSKVFYISKLDEENADFFEVVNQLVQTFGNKAIA
                     FQIPIIENEKFTGIVDIVNMKAKRFEKDRIIEADIPSGMSSKVDELREALKEAVAETD
                     EELMEKYFSGEEFTREEISKGLRFGILEGSIVPVLCGASINNFGVKPLMDAIIEYLPF
                     PADLTVVRAKKAGSDETVELKISEDESFSALVFKTIADPFVGKITLFRVYSGTLTPDS
                     VIYNSTTEKTEKIGQLFVLRGKKQIPTDKLIAGDIGGVAKLQNTNTNDTLSDTSKPVV
                     LDEIIFPEPSITLAIEPKSKGDEEKISLGLQRLQEEDPTFRVEINSEVRQTLISGIGE
                     QHIDVIISKLKSKFGVSVNLIDPKIPYRETIKKKVKAEGKHKKQTGGHGQYGHVWVEF
                     EPGETEDLIFDEKVFGGAVPRQYFPAVEKGLRESISKGVLAGYPVVNLKATLVDGSFH
                     PVDSSEMAFKIAASLAYKKGLEDASPVLLEPIIHVEVYVPDNYMGDIIGDLNKRRGRI
                     LGMNPQHDGTQQIVAEVPQAEMFKYATDLRSMTQARGSFKEWFERYEEVPPMISQKVI
                     DEVRKATGDEGEDS"
BASE COUNT          927 a          391 c          631 g          817 t
ORIGIN      
        1 gcacttatgt tataataaat gctattaagg aaataaaacc ttagtggttt tatctttatg
       61 catttattga aacggtgtga agcaatccga ttagcggatt gcacatatgt tataataatg
      121 gctgctaagg gtaggtacct gtaaaattta tatttaggca ttttagtaat aattttggat
      181 atatgatggg tataattctt tatataatga gttttatatt cttgtgtata aaatattatg
      241 tatcgttcct ttgaagcaag tgctgctgca cattggtgtt tttaccgagg atgagacaac
      301 ggcacatatt atatataaaa aatgcagaga atacggtaag gactatcgga gtttacgcct
      361 gcggagttag taggttgcga ggacaaagaa gcaggaaaat ctattagtga taatagaaac
      421 tcgtcacttt agtgatggga ggttcgcatg ctggtattgg tagtactgaa atcctgaaaa
      481 tacatattaa gggggagagt ttgtaatatg aaagattata ctgttgaaaa attacgaaat
      541 gtttgtttat tgggccatgg aggttcaggc aagactacat tggccgaagc aatgcttttc
      601 aataccggtg ttttggatag gtttggtaaa gtgcctgacg ggacaactac aactgacttt
      661 gatcctgaag agattaaaag aaaaatttct ataaacaccg ctatagctcc ttgtgagtgg
      721 aatgggcata agattaatgt aatagatact cctggctttt ttgactttat aggcgaatta
      781 aagcaaggct taaaagtcgc tgaaagtgct gtaattcttg ttcctggtaa gggaggagta
      841 gctgtaggta cggagaaatc atggcaatac gcaaatgagc atggtatgtc aaaagtgttt
      901 tatatcagca agcttgatga agaaaatgct gatttttttg aagttgttaa tcaattggtg
      961 cagacttttg gcaataaagc tattgctttt caaataccta ttatcgaaaa tgaaaaattt
     1021 acaggtattg ttgatatagt taatatgaaa gcaaaaagat ttgaaaagga cagaataatt
     1081 gaagctgaca tcccatcggg catgagcagc aaggtggacg aattaaggga agcattaaaa
     1141 gaagcagtag ctgagacaga tgaagaactt atggaaaaat atttttcggg agaagaattt
     1201 acccgggaag aaattagtaa aggattgcgt tttggaatat tggaaggctc aatagtacct
     1261 gttttatgtg gagcttcaat aaacaatttt ggtgtcaagc ctcttatgga tgcaattatt
     1321 gaatatttac cgtttcctgc tgatttaaca gttgttagag ctaaaaaagc aggtagtgat
     1381 gaaacagttg aattgaaaat ttcggaagat gaatcttttt cagcattagt atttaaaaca
     1441 atagctgatc cctttgtggg gaagataaca ctttttaggg tgtattcagg taccttgact
     1501 cctgatagtg ttatatataa ttctactaca gagaaaactg aaaaaattgg gcaactattt
     1561 gttttaagag gtaaaaagca gatacccact gacaaattga ttgcagggga tatcggaggt
     1621 gttgccaaat tacagaatac caataccaac gacacgcttt ctgacacttc taagcctgtt
     1681 gtattggatg agataatttt tcccgaacct tctataactc ttgctattga gccaaaatct
     1741 aaaggtgatg aggaaaaaat aagtttaggc ttacaacggt tacaggaaga agatccgact
     1801 ttcagagttg aaataaatag tgaggtacgc cagacactta tttcaggtat aggagagcaa
     1861 catatagatg ttataataag caagttaaaa tccaaatttg gtgtttcagt taacttaatt
     1921 gaccccaaaa taccttatag agagactata aaaaagaagg ttaaagctga aggcaaacat
     1981 aagaaacaga cagggggcca tggacaatat ggccatgttt gggttgagtt tgagcctgga
     2041 gaaacagaag acctcatttt tgatgaaaaa gtattcggtg gcgctgtccc aagacagtat
     2101 tttccagctg ttgagaaggg ccttcgtgaa agtataagta agggagtatt ggcaggatat
     2161 cctgttgtca accttaaagc aacccttgtt gatggttctt ttcatcccgt agattcctca
     2221 gaaatggctt ttaagattgc tgccagcctg gcatacaaaa aaggactgga ggatgcttca
     2281 cctgtgcttc ttgaacccat tatacatgtc gaagtatatg tacctgataa ctatatggga
     2341 gatatcattg gagatttgaa taagcgcagg ggcaggatat tggggatgaa tcctcagcat
     2401 gacggtaccc aacagatagt tgctgaagta cctcaagctg aaatgtttaa atatgctact
     2461 gatttaagat ctatgactca ggcaagggga tcttttaaag agtggtttga gaggtatgag
     2521 gaagtgcctc ctatgataag tcagaaagta attgatgagg tcagaaaagc aacgggagat
     2581 gaaggagaag acagttaaga gtagaatatc ggaataaaat tcatttattg ccagcatggg
     2641 tgcattttct tcagaaaaag gaaaaatgca cccatttgca aattttatac catttacaaa
     2701 ggtgcatttt ataaaagtga agaaatattt accaggagct gacgttacgc cgcttagagc
     2761 ctttaa
//
LOCUS       DAOSDD010000399         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12905_length_2757_cov_3.790526, whole genome shotgun sequence.
ACCESSION   DAOSDD010000399 DAOSDD010000000
VERSION     DAOSDD010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12905_length_2757_cov_3.790526"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..258)
                     /locus_tag="PK733_19565"
     CDS             complement(10..258)
                     /locus_tag="PK733_19565"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOJ12769.1"
                     /translation="MQYGLVDDVSHAFGPYSRQAGDAIARSDKWLSRCIAELRKHEHG
                     IIILADHGQHEFKRSDGTVGGTHGEDIIEDCIVPLTWT"
     gene            579..1298
                     /locus_tag="PK733_19570"
     CDS             579..1298
                     /locus_tag="PK733_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12770.1"
                     /translation="MIKITNLQIELNSIIAAYNALVKGIDEAALDNRDRAYGGIIRAG
                     KGKLVESIARQLVRSAWCTVLNQPGSRLTMDKTKFRIRLKDDYINRMKEPYVKNYLVK
                     NKSTIAYKFGTDVHVYIDGRLVIPIECKAYTENAMMKRILFDAALMQEAKGINKYYLL
                     QLESQLGGDYCELNDITYGSPSTHTLISYFDVDLKIITLLKGERKVDQPIHKPEYYKP
                     LTLQRLEWAANMLAADLKQFT"
     gene            complement(1311..2477)
                     /locus_tag="PK733_19575"
     CDS             complement(1311..2477)
                     /locus_tag="PK733_19575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ12771.1"
                     /translation="MGSTKYIELKMNIENQLDNNEIEAISLCNQIFPKNDFYFKVEDN
                     VLYAYIKQLDLEKLLSCIAKSDIDSNSILYSIYENINNIGSYGIKGGKRVFVDFNKER
                     KVANRKSKEEERNKFFYARNNEFSKTNNVLPEKFNNQIICADSEEFLKLLPDNCIDLI
                     FTSPPYNFGLDYENSDDSNHWERYFDKLFRIFKECIRVLKYSGRIIVNVQPLFSDYIP
                     SHHIISNFFIQNKMIWKGEILWEKNNYNCKYTAWGSWKSPSSPYLKYTWEFIEIFCKG
                     DLTKRGDKENIDITADEFKQWVVAKWSIAPERRMKEYSHPAMFPEQLVERVLKLFSFK
                     GDIIFDPFNGVGTTTVVAYKLGRNYVGLDISEEYCQKAKERVANTLINMSLFDL"
BASE COUNT          911 a          500 c          395 g          951 t
ORIGIN      
        1 tttaatttat tatgtccatg ttagcggcac aatgcaatcc tcaataatat cttctccgtg
       61 agtaccaccc actgtgccgt cagacctctt aaattcatgt tgtccgtgat ccgcaagtat
      121 tataatacca tgctcgtgtt tacgaagttc agctatacac cttgataacc acttatcaga
      181 tctagcaata gcatccccgg cttgtcttga ataagggcca aacgcatgag atacatcatc
      241 aacaagtcca tattgcacaa taataaactg ttttatgttg gatgttcacg agcaaggaag
      301 aacttaatca tcttgcttga acgattgaag cttaaggggg atattgaatt acaaaaaaga
      361 aagtttacta cacttggttt cgttgtttcg tagcaacgtg ttcaattcac tccccgcacc
      421 caaaacaata atggatcttt gtctatagca tctatcttta cctaacttct cataatttgt
      481 cctgatactt tttgtacata aaaaacaaaa aaaccaaaat aatatgatca tccgaccttt
      541 ttaagaatgc cggataatta tattatttaa taaaaaggat gataaaaatc acaaatttac
      601 aaatcgaact aaatagtatt atagctgcat ataatgcatt ggttaaaggt atagatgaag
      661 cagcccttga taatagagac agggcatatg gtggcattat tagagcaggt aagggtaagt
      721 tggttgaaag tatagcaaga caattagtca ggtcagcatg gtgtacagtt ttaaatcaac
      781 ctgggtccag attaactatg gacaaaacaa aattcagaat cagattaaaa gatgactata
      841 taaaccgaat gaaagagcca tatgttaaga attatcttgt aaaaaataaa tcaacaatag
      901 catataaatt tggtaccgat gttcatgtat atattgatgg caggttagtt atacctattg
      961 aatgtaaagc atatactgaa aatgcaatga tgaaaagaat tttatttgat gcagcattaa
     1021 tgcaggaagc aaaaggaata aataagtact atctattgca gcttgaaagc cagcttggcg
     1081 gagattactg cgaattaaac gatataacat atggaagtcc ctcaactcat accttaatat
     1141 cctactttga tgtagatctg aaaataatta ccttgctaaa aggcgaacgg aaagtggatc
     1201 aacccataca taaaccagag tactataaac cattaacact tcaaaggttg gaatgggctg
     1261 ctaatatgtt agctgcagat ttaaaacaat ttacataaat gattaaatga ttatagatca
     1321 aacaatgaca tatttattag agtatttgct accctctctt ttgctttttg gcaatattct
     1381 tcagaaatat ccaaccctac atagtttctg cctaatttat aggcaactac cgttgtggtt
     1441 ccaactccgt taaatggatc aaaaataata tcacctttaa aactgaataa ttttaaaact
     1501 ctttcaacca actgctctgg aaacattgca ggatggctat attctttcat tcttctttcc
     1561 ggagcaatac tccattttgc aaccacccac tgtttgaatt catccgcagt tatatcaatg
     1621 ttttctttat ctcctctttt cgttaaatct cccttgcaaa aaatttcaat aaactcccat
     1681 gtatatttta aatatgggct agaaggactt ttccaacttc cccatgcagt gtatttgcaa
     1741 ttatagttat tcttttccca taatatttcc cctttccaaa tcattttatt ctggatgaag
     1801 aaattactaa ttatatgatg acttggtata tagtcggaaa ataggggttg tacatttaca
     1861 attattctac cactatattt taaaactctg atacactcct tgaatattct aaagagctta
     1921 tcaaaatacc tttcccaatg gttactatca tcagaatttt cataatcaag tccaaaattg
     1981 taaggcggtg atgtaaatat taggtcaatg cagttatctg gcagtaattt aagaaattcc
     2041 tcgctatctg cacatattat ctgattatta aacttttctg gtaatacatt gtttgtttta
     2101 ctaaattcgt tattcctggc ataaaaaaac ttatttcgct cttcttcctt gctcttgcgg
     2161 ttcgctacct ttctctcttt attaaaatct acaaatactc ttttcccgcc ttttatccca
     2221 taacttccaa tattgtttat attttcatat attgaatata aaatactatt actatctata
     2281 tccgatttag caatacagct tagtaatttt tctaaatcaa gttgctttat atatgcatac
     2341 agaacattat cttcaacttt aaaatagaaa tcattttttg ggaaaatctg attgcagaga
     2401 gatatagctt ctatttcatt attgtcaagt tggttctcaa tattcatttt taactctata
     2461 tattttgttg aacccataaa tttttctcct aatccgtatt ttttaaattt acagctttgg
     2521 attttttcat tattctgtct ttagatttat tacatgctga aggtataatc aacatattcc
     2581 cgatcttttt agatgcttca tgccggtttt aagcacacaa aacttgagct cttctatgcg
     2641 gtatgtccta tctttcaacg gcttctccag cactcatata ctctttaata tgatcaaata
     2701 ctatcaaatg aaattatgtg cttaattata tgccagatgc cgaacaatgt caattat
//
LOCUS       DAOSDD010000400         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_12975_length_2746_cov_5.198439, whole genome shotgun sequence.
ACCESSION   DAOSDD010000400 DAOSDD010000000
VERSION     DAOSDD010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12975_length_2746_cov_5.198439"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2103
                     /locus_tag="PK733_19580"
     CDS             <1..2103
                     /locus_tag="PK733_19580"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ12772.1"
                     /translation="IERVWKYDGSSLYTVSILNKLNNSQLINQSQLINQHINNEIEFI
                     FNANKTEFSYEGLTCSIRAKDKPYFMELADVKVTEVTDSIYSVPYLEVMFYLKDKVHR
                     VDIKRYALIYENTPAIKTYMEIRTCNFPMGEFYDGFKLNILDNYPVNHDYEAGDMFSC
                     EFFTRTDHTNHLVEKRKNPEGFDRGNLIIYSGNKTRSIVSKSTDNINKTTNITTNITT
                     SKTSSKTTNKTINKSVNESGFFILKESPCFSDQRPEAEGNFYIGPDGIKTLGWGIRPE
                     EFRMNEFMRTYASVIGVFTGDSNDGLLALKKYTQARAVIKPERDYMIMANPWGDRQCL
                     EHMGEEFVLNEIEACAKLGATHYQLDDGWQKRGSLKRLSDNEALEEDFWDMNIERFPS
                     KFDKIAKKAKELNVELALWFAPDFNRLFRNYKEQAELLYSMYKNYGIKKFKIDGVSLR
                     SKEAEENLEKLLKILKEKSKGDISFNLDTTNNPRGGYFMFQEYGNVFLENRYTDWSNY
                     YPWLTLKNLWDLSAYVPTQKLQIEFLNIDRNKDKYKKEDILAPVNYSYEYVFAITMFA
                     NPLCWFEPSALDSEPMERYSKIISLYKKYRDDIFEGSIFPIGDRPSGYSWTGFQSHNS
                     SKNRGFIIIYRECSEESVCSIKPLFIEGFNIEMRSLSDENENIVIKDYDGRGIDFHLE
                     KQNSFRLCEYILDSSKNE"
     gene            2218..>2746
                     /locus_tag="PK733_19585"
     CDS             2218..>2746
                     /locus_tag="PK733_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HOJ12773.1"
                     /translation="MNTYKPNEFAEMIGVSVKTLQRWDNDGKFKAFRNPSNRRYYTHN
                     QYVEYMGKIIQDKDKRKTIIYTRVSTNGQKDDLKNQSEFLKQYANAKGIIVDEVFEDI
                     GSGLNYNRKKWNKLLEDCMLGLIKTIIISNKDRFIRFGFEWFERFLKLNRVELIVVNN
                     ESLSPQEEMIQDLISI"
BASE COUNT         1022 a          359 c          549 g          816 t
ORIGIN      
        1 atagaacggg tctggaaata tgatggaagc agcttatata ctgtatcaat actaaacaaa
       61 ctaaataatt cccagctaat taatcagtcc caactaatta atcagcatat caataatgaa
      121 attgaattta tcttcaatgc taataagact gaattttcct atgaagggct gacatgcagt
      181 attagagcaa aagataagcc atattttatg gagttggctg atgtaaaagt aactgaggtg
      241 acagattcga tctattctgt accatatctt gaggttatgt tttatttgaa agataaagtt
      301 cacagggtag atattaaaag atatgcgtta atatatgaaa atacccctgc cataaaaaca
      361 tatatggaaa tccgcacgtg caatttcccc atgggcgagt tttatgacgg gtttaagtta
      421 aatatattgg ataattaccc ggtaaatcat gactacgaag caggtgatat gttttcttgt
      481 gaattcttca ccagaacgga tcatacaaac cacttggtgg aaaaaaggaa aaatcctgaa
      541 ggttttgaca ggggaaatct tattatatat agcggtaata aaaccaggag cattgttagc
      601 aaaagtactg ataatattaa taaaactact aatataacta ctaatataac tactagtaaa
      661 acttctagta aaactactaa taaaaccatt aataaaagtg ttaatgagag cgggtttttc
      721 atactgaagg aaagtccttg cttttccgac cagcgtcctg aggcagaggg aaatttctat
      781 attggaccag atggcattaa aacccttgga tggggcataa gacctgaaga gttcaggatg
      841 aatgagttta tgagaacata tgcatctgtc ataggagtgt ttaccggaga tagcaatgac
      901 gggcttcttg cattaaagaa atatactcaa gcaagagctg ttattaaacc tgagagggat
      961 tatatgatta tggccaatcc atggggggat aggcagtgcc tggagcacat gggtgaagaa
     1021 tttgttttaa atgaaattga agcatgtgca aagctgggag ctactcatta tcagttggat
     1081 gatgggtggc agaaaagagg tagccttaag cgcctttcag ataatgaagc tttagaagaa
     1141 gatttttggg atatgaatat tgagcgtttt cccagcaaat ttgataaaat tgcaaaaaaa
     1201 gcgaaagagc tgaatgttga gcttgcttta tggtttgctc ctgattttaa caggcttttc
     1261 agaaactata aggaacaggc tgaattacta tatagcatgt ataaaaacta tggaattaaa
     1321 aaattcaaga tagatggtgt aagcttaaga agcaaagaag ctgaagaaaa tcttgaaaag
     1381 cttcttaaaa tattaaaaga aaagtccaaa ggagatatat cttttaatct ggatacaaca
     1441 aacaacccaa gaggcgggta ctttatgttc caggagtatg gaaacgtttt ccttgaaaac
     1501 cgttatacag attggagtaa ttactacccc tggcttactc taaaaaatct atgggatctt
     1561 tctgcatatg ttccaactca aaaactgcag atcgaatttt taaacataga ccgaaataaa
     1621 gacaagtata aaaaagagga tatattggca cctgtaaact attcctatga atatgttttt
     1681 gctattacca tgtttgcaaa tcccctgtgt tggtttgaac catcagccct ggacagcgag
     1741 cccatggaaa ggtacagtaa aataataagc ttgtataaga aatatcgtga tgatattttt
     1801 gaaggcagca tatttccaat tggtgacaga ccatccggtt attcctggac gggttttcaa
     1861 tcccacaatt catctaaaaa cagaggattt atcattattt accgtgaatg cagtgaagaa
     1921 tctgtttgca gtatcaaacc gttatttata gagggcttta atatagaaat gaggagtttg
     1981 agtgatgaaa acgaaaatat agtaattaag gactatgatg ggaggggaat agatttccac
     2041 cttgagaagc agaatagttt cagattgtgt gagtatatac ttgattccag taaaaatgaa
     2101 taatattatt gtattatata agtgaaaagt tattatttaa aggagatgta aactgtaaca
     2161 aaatatattg acaagcatag acatatatgg tagtatatag gcaggaggtg tttatgtatg
     2221 aacacatata aaccaaatga atttgcagaa atgataggtg tgtcagttaa aacattgcaa
     2281 agatgggaca atgatggcaa atttaaggct tttagaaatc cttctaatag aaggtattat
     2341 actcataatc aatatgttga atatatgggt aaaattattc aagacaaaga taaaagaaaa
     2401 accattattt atacaagagt ttcaacaaat gggcaaaaag atgatttgaa aaatcagtct
     2461 gaatttttaa agcagtatgc taatgcaaaa ggtataatag ttgatgaagt atttgaagat
     2521 attggcagtg gcttaaatta caatcgtaaa aaatggaata agttattaga agattgtatg
     2581 ttaggtttaa taaagacaat tattatttct aataaagata gatttataag atttggattc
     2641 gaatggtttg aaagattttt aaaattaaat agagtagaat taatagttgt aaataatgaa
     2701 agtttgtcgc cacaagaaga aatgatacag gatttaattt caatta
//
LOCUS       DAOSDD010000401         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13154_length_2716_cov_6.721909, whole genome shotgun sequence.
ACCESSION   DAOSDD010000401 DAOSDD010000000
VERSION     DAOSDD010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13154_length_2716_cov_6.721909"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..929
                     /locus_tag="PK733_19590"
     CDS             39..929
                     /locus_tag="PK733_19590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ12774.1"
                     /translation="MFNQIIVRKAGIKDAAIISSIGVSSWQAAYRGIISDAYLDSLSV
                     EKKEVQLAKSFAIPENQFAVAEIDNQAVGMMCFRPDYRKENIKEQDCWEIEAIYVLPQ
                     YWRIGIGRALIKYAFRHMKSININSCGLWVLVENHRARKFYETIGFYPSGIERPISIG
                     GKELIEMHYTIDLNDMLAKKIYNMEMQLLRPEVRISSEKLAELLWDDMIEFCSSGFVY
                     QYKKNDVIEKSLNSSTLKWKIKDFKIKCLSDDIILVTYMLDKNSYTSTKPNHSLRSSI
                     WKSTDGKWKMFFHQGTPVVE"
     gene            973..1503
                     /locus_tag="PK733_19595"
     CDS             973..1503
                     /locus_tag="PK733_19595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ12775.1"
                     /translation="MKELKGQTVLLRDIKESDIDDRHILGKNDEFIYMCGGNRDGRTL
                     YPDREHWVAWYESFKKNECAWIIEYNGKCIGSARLHHISETDRSATYAIGIFDVTLFS
                     KGIGSEVTKLVLEYAFKEMKLHRVDLKVLDYNKRAIRCYEKCGFKIDGILRDSAFIEG
                     SFHSDIIMSILEDEWK"
     gene            1514..1681
                     /locus_tag="PK733_19600"
     CDS             1514..1681
                     /locus_tag="PK733_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12776.1"
                     /translation="MQTWYKSFGKETAAPKRPEYIWKKSLLTIFKQLVSEDFSELRLC
                     VIRDRMTESHD"
     gene            2059..2475
                     /locus_tag="PK733_19605"
     CDS             2059..2475
                     /locus_tag="PK733_19605"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12777.1"
                     /translation="MYSAGLRVGEVVRLKINDIDSERMLIHVVQGKGRKDRYTTLSEV
                     ALTELRNYTKEYNPETWLFPGQNPKKFITERTVQRVFENAKMKAGIKRKVSVHSLSYP
                     NLYKIQTFGFFKTCFCNTFVGRRNRFKVYTGIARPF"
     gene            2423..2575
                     /locus_tag="PK733_19610"
     CDS             2423..2575
                     /locus_tag="PK733_19610"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12778.1"
                     /translation="MEGGIDLRYIQELLGHSSSKTTEIYTHVTRKSIQNIVSPLDMIV
                     RRDKKT"
BASE COUNT          942 a          365 c          595 g          814 t
ORIGIN      
        1 atatgtgttt attgtaaatt aggaaggagg tcaaataaat gtttaatcaa attatcgtca
       61 gaaaggccgg cattaaagac gctgcaatta taagttcgat tggagtttct tcatggcagg
      121 cagcatatcg aggaattatc tctgatgcat atcttgattc tctttcagtt gaaaagaaag
      181 aggtgcagtt ggctaaaagc tttgctatac cggaaaatca atttgctgtg gctgaaattg
      241 ataatcaggc agttgggatg atgtgttttc gtcctgatta tagaaaagaa aatattaagg
      301 aacaggattg ctgggaaatt gaagctattt atgttttacc acaatattgg agaattggaa
      361 taggtcgagc tttgattaaa tatgcttttc ggcacatgaa atcaattaat attaatagct
      421 gtggtttgtg ggttttagta gagaatcacc gtgcaagaaa gttctatgag actataggtt
      481 tttatccatc aggtattgaa agaccgatat cgataggagg gaaagaactt atagagatgc
      541 attatactat tgatcttaat gatatgttgg ctaagaaaat ttataatatg gagatgcaat
      601 tattgagacc ggaagttaga atatcatctg aaaaactggc agaattgctt tgggatgata
      661 tgattgaatt ttgcagcagt ggattcgtat atcaatataa gaaaaatgac gttatagaga
      721 aaagtttaaa ttcaagtaca ttaaaatgga aaataaaaga tttcaaaatt aagtgtttat
      781 cagatgatat aatccttgta acatatatgc ttgataaaaa tagctacaca agcactaaac
      841 ccaatcactc tcttcggagc tcaatctgga aatccactga tggaaaatgg aaaatgtttt
      901 tccatcaagg cacaccggtt gtagaataaa ttgcattcat tttattaaca ataccagaaa
      961 agaggtaggt atatgaaaga actcaaaggg caaacagtgc tcttaaggga cataaaagag
     1021 agtgatattg atgataggca cattcttgga aaaaatgatg aatttatcta tatgtgtggc
     1081 ggcaaccgag acggaagaac actttaccca gaccgtgagc attgggtagc ctggtatgaa
     1141 agttttaaga aaaatgagtg tgcatggatt atcgaatata atggtaaatg tattggaagc
     1201 gcaaggctgc atcatatatc tgagactgat cgtagtgcaa cctatgcaat cgggattttt
     1261 gatgttacgc tgttttctaa aggtataggc agtgaggtta caaagcttgt tctggaatat
     1321 gcttttaagg aaatgaagct gcatagggtt gacttaaagg ttcttgatta taacaagcgt
     1381 gcaatacggt gttatgaaaa atgcggtttc aagatagatg ggatattaag agacagcgca
     1441 tttattgaag gctctttcca ttcagatatt attatgagta ttctggagga cgaatggaag
     1501 tgagtgggat ttaatgcaaa catggtataa atcttttggg aaagaaacag ctgctcccaa
     1561 gcgcccggaa tatatctgga aaaaatcttt gcttacaata tttaagcagc ttgtttcgga
     1621 agatttttcg gagctaaggc tgtgtgtaat tcgggatcgc atgactgaat ctcatgactg
     1681 aatctcatgt tttgtttggt tctgttttgg aggggttgtt gaaagtagcc tttttgcttc
     1741 atcacctata ttttccttgg cgtacacatc tgcgatggac atttgataga tcctgtcttc
     1801 tagtttcaaa ttttggatca agtattgaca ggaaattatc atataacagt tggcataata
     1861 aaattaatag tataggcaat ctaattgatg aatacaaaaa atatattgct gctcatgttc
     1921 ttttgcccga gataaacatt tggagtgcgt agtattgaga gagaagaaat aggttgaagt
     1981 agcagggttg aggagatttc ccttcctgct ttaagggatg tattagagaa taagaaacac
     2041 cgagcaatac catttacaat atactcagca ggattaagag taggagaagt agttaggtta
     2101 aagatcaatg atattgattc tgaaagaatg ctgattcatg ttgtacaggg taaaggtagg
     2161 aaagatagat atactaccct atctgaagta gctttaacgg aattaagaaa ctatacaaaa
     2221 gagtataatc ctgaaacatg gttatttcct ggacaaaatc ctaaaaaatt tattaccgaa
     2281 aggactgtac agagggtatt tgaaaatgct aaaatgaaag caggaataaa gagaaaggta
     2341 tctgtacata gtttaagtta tccaaacctt tataaaatcc aaacgttcgg attttttaag
     2401 acatgctttt gcaacacatt tgttggaagg aggaatagat ttaaggtata tacaggaatt
     2461 gctaggccat tctagttcaa aaacgactga aatctataca catgtaacta gaaaaagcat
     2521 acaaaacatt gtaagtccac tagatatgat agttaggagg gataaaaaaa cttagcctaa
     2581 aggggcatta gcgacatata tcgtaaaata tcccatattg tgggtattag cgacagtcga
     2641 taatatctat aataaagaat gttaggcgac agatgtcggg aatacaatag ataagcagac
     2701 atcgctcgct gcgctc
//
LOCUS       DAOSDD010000402         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13252_length_2696_cov_5.087467, whole genome shotgun sequence.
ACCESSION   DAOSDD010000402 DAOSDD010000000
VERSION     DAOSDD010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_2696_cov_5.087467"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK733_19615"
     CDS             complement(<1..474)
                     /locus_tag="PK733_19615"
                     /inference="COORDINATES: protein motif:HMM:NF024673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOJ12779.1"
                     /translation="MGHQASTVLRIIDLNPSTYYYTITKKSKEKNENGGRPIPGYSYT
                     ISGKKICDMQIKEWIITLIEGDCFAYGYYKLTIELQRTYKLIINKKKVYRLCKELGVL
                     RPQRKIKARYPRKLAQNRTVTAPNQLWETDLKYGYIVGEDRFFYIQSIIDVYDREI"
     gene            complement(498..827)
                     /locus_tag="PK733_19620"
     CDS             complement(498..827)
                     /locus_tag="PK733_19620"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ12780.1"
                     /translation="MGVKKHSDEFRRQVVKEALETGNSSLVGRKYNINPSMVARWVRE
                     EKPNPINNMTKKALQTASVLTEDPKEVKKAIEQNTQLKRLIGEKELEIEILRDLLKKM
                     KIPLPSR"
     gene            1384..2397
                     /locus_tag="PK733_19625"
     CDS             1384..2397
                     /locus_tag="PK733_19625"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ12781.1"
                     /translation="MKQEKNYHYDVKEVFRIFYTDEFMLPPEERIIPNEHYHDVYEIF
                     YIINGNGAHYINNKKIEIKKHDMVFVDKYHIHKERYYDNDCKERIIFQFNDDVLNSFV
                     NTRIKEEVINLFELKVLSYPDSFNEYLINTFKNRILPEYNKASNILNYTICKLIFLEV
                     LLEILKLKENGEVIIKKEEVQNKEDKSTNSNGHIEENKIVKNSTHNANNTNNANNTNN
                     TNNTKKIDRSKEDIVYEIINYIKLNYETDISLEQLSKDYYISKYYLCRKFKEISGSTI
                     IEFVMNNRLTEAEKLIKHSKLNIAEISRQVGFKNCNHFINVFKRKYGYSPNVFRENYI
                     KSL"
BASE COUNT          951 a          414 c          411 g          920 t
ORIGIN      
        1 aatttctctg tcatacacat caataattga ctgtatatag aaaaacctgt cttctccaac
       61 tatataacca tacttaaggt ccgtttccca caattgattt ggagcggtaa ctgtacgatt
      121 ttgagccagt tttcggggat accttgcttt tatctttctc tggggcctta acactcctaa
      181 ttccttacac aaccggtata ctttcttctt attaataatc agtttatacg ttcgttggag
      241 ttctatggtc agcttataat agccatatgc aaagcaatca ccttcaataa gtgtaattat
      301 ccactcttta atttgcatat cacatatctt tttaccactt atggtgtatg aataccctgg
      361 aataggtctg ccgccattct cattcttttc tttacttttc ttagtaattg tataataata
      421 cgtagacgga ttaaggtcta ttattcttag tactgtagat gcttgatgcc ccaaattaat
      481 ccatttattt gcaatttcta ccttgaaggt aacggaattt tcattttttt taataggtct
      541 ctcagtattt ctatttcgag ctccttctcg ccaattagcc ttttaagctg agtattctgc
      601 tcaatcgctt tctttacttc cttaggatct tctgttagaa ctgaagctgt ttgcaatgct
      661 ttctttgtca tattgttgat cgggtttggc ttttcctctc gaacccacct agccaccata
      721 gaagggttta tattatattt ccttccgaca aggctcgaat tacctgtttc taaagcttct
      781 ttcactacct gtcttctaaa ttcatcagaa tgctttttaa ctcccatttt tcttctcccc
      841 tttgcttgtt tctattatac aagctttgag tctaattgtc caaggtgatt agggggctta
      901 tgagatagga ctaacaccat caagctttag agatacaaaa tagttagata atattcaaat
      961 tattagagaa gtcatataaa gaacatataa aggcactcct gcatacacag cttttgcaaa
     1021 acatgataac gattttattg aaatgatgaa ttcataagaa ataaacgata aatataaatg
     1081 gcaaatataa actgtatgtt ggatgaaata aaacctgaaa tagcagttgt gtgctgctat
     1141 ttcgctaatc aggcgaaggc agccggtgat gcactaatat tttaacttct tattcatttt
     1201 tcccgtgata tatgatataa tttttttacc tctaaaacat taaattttag cttcataatc
     1261 ataccacaaa acttggatta atttgcagaa cttggagcaa tttatatggc ttggatgaat
     1321 taaactaatt attttctgca tattatttgt acaaatatgt acagggaagt gagagttttt
     1381 accatgaagc aggagaaaaa ttatcattat gatgtaaaag aggtatttcg gatattttat
     1441 actgacgaat ttatgctgcc ccctgaagaa cgaataatac ccaatgaaca ttaccatgac
     1501 gtatatgaaa tattttatat tataaatggc aatggagctc attatatcaa taataagaag
     1561 attgagataa aaaaacatga tatggtattt gtcgataaat atcacattca taaagaaaga
     1621 tattatgaca atgattgtaa ggaacgcatc atttttcagt ttaatgatga tgtcttaaat
     1681 agctttgtta acacaagaat aaaggaagaa gtaattaatt tatttgagtt gaaagtgctg
     1741 tcttatccgg atagctttaa tgaatatctt ataaatactt tcaaaaacag gatattgcct
     1801 gaatataata aagcatctaa tatattaaac tatactatct gcaaattgat atttttggag
     1861 gtattacttg agattttaaa gcttaaagaa aatggggaag taattataaa aaaagaagaa
     1921 gtgcaaaaca aagaagacaa atcaaccaat tccaatggtc atattgagga aaataaaatt
     1981 gttaaaaata gtactcataa tgcaaataat actaataatg caaataatac taataatact
     2041 aataatacta aaaaaataga cagatctaaa gaagatatag tatatgaaat aataaattat
     2101 attaaactta actatgaaac agatatttct ttagaacaat tatctaagga ttattacata
     2161 agcaaatatt atttatgccg taaatttaaa gaaatttcag gttcaactat tatcgaattt
     2221 gtaatgaata ataggctaac tgaagctgaa aaacttatca aacattcaaa gcttaatatc
     2281 gctgaaattt cccggcaagt aggttttaaa aactgtaatc attttataaa tgtatttaaa
     2341 aggaaatatg gttattcgcc caatgttttc cgggagaact atataaagag tctttgatag
     2401 attctggata cagtggcaaa acctaagcaa taatcatata tatttgattg cacaaagcaa
     2461 gattacttga cttcaaatat gcaggaaagc agcaaaaatg agcaccgctc ttaaaattat
     2521 agcaattatc atatatacat gaaatgtttt acagcaagat tttttaatga caatgtaagg
     2581 gaaaaatggt actataatta caatccaagt tcagaggaaa taagtgataa aatttattag
     2641 ttttaggaaa cacagcggga atcctctcta ttttaatgga gcagatgaaa gctgtg
//
LOCUS       DAOSDD010000403         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13328_length_2684_cov_5.310004, whole genome shotgun sequence.
ACCESSION   DAOSDD010000403 DAOSDD010000000
VERSION     DAOSDD010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13328_length_2684_cov_5.310004"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1862
                     /locus_tag="PK733_19630"
                     /pseudo
     CDS             396..1862
                     /locus_tag="PK733_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(2041..2370)
                     /locus_tag="PK733_19635"
     CDS             complement(2041..2370)
                     /locus_tag="PK733_19635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOJ12782.1"
                     /translation="MYCQKHMMQLHQDYSKFVEKDTIIVAIGPENADSFEKYWKNNEL
                     EFYGIPDPNHEVLKLYGQKVNLFKLGRMPAQMLVDKEGVLRFVHYGHSMQDIPENKEI
                     LRLIYSL"
     gene            complement(2371..2502)
                     /locus_tag="PK733_19640"
     CDS             complement(2371..2502)
                     /locus_tag="PK733_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12783.1"
                     /translation="MASVEVNREAPDFEMKDFKGDIFKLSAFKGKKNVLIVLNRGFV"
     gene            complement(2490..>2684)
                     /locus_tag="PK733_19645"
     CDS             complement(2490..>2684)
                     /locus_tag="PK733_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12784.1"
                     /translation="EDQESLDCCIHSCCYTYILDQINRIIFLPPGIFRRPFIGDFLDG
                     YNTMEHANKGCTGGNIKWQV"
BASE COUNT          952 a          527 c          489 g          716 t
ORIGIN      
        1 agcagttaaa tacaatggaa tcaatgggta taccatttct gtgctcatat ctacaaaaaa
       61 actaaccagt cccaataaaa taatatttga atttagaaat ctgtttttca tagcacacct
      121 tcgatttttt ttattttatg aatcttacga tacccctttg ctttaataaa atcaagatgc
      181 atactgctca gcttgaaaaa agataatatt aaaaagcagc ggggacggtt cgagaccagt
      241 gaaaaagtcc tgaactagtc ccactcaaaa tagaatagag ttatgcgatc agaaccttaa
      301 aaccattgta attactcgat ttcacataaa aatatccccc cgtttatggt ataatgtaag
      361 tagcgaacca acattaccaa aaggaggata tttctatgtt acgaacaaag cctaagcaat
      421 tgtcgttcca ctcatcatta tacaataaaa tacccgagaa tcatatccta aagaagatag
      481 attctgtcgt agactttagc ttcattaatc gtctcctttc aagcagctac tgcaaggagt
      541 tcggaagacc cgctaaagaa cctgaattga tgtgtaaatt gctgttttta caacacctat
      601 acaatctttc agacgagaga gtcatcgaag aagcagagct taatttagca tatctttact
      661 tcctaggagt caatccagag gaaagtcttc ctgacaaaag cctcttatcc aaattcagga
      721 cccatagatt aagagaatcc actcttgatg aaatcataac tgagattgtg aaacaatgcg
      781 tagaaagagg tatcataagc tcatcaagcg taagtataga tgctacacac atagaagcaa
      841 atacaatcaa aaaaacacct gagcgattga tgaaacatct tgcgcaggaa ataatcaaaa
      901 gctatgaaga agatacaaaa gaagaactaa aagacttgcc acaagttcct gaatacaagg
      961 aaatcaagga tcataaagaa gccaagtctg tcatgaaaga ctatctagaa acagtaatag
     1021 agaaagtagg cagctgtata accccggata atgcaaacac gaataaagcg atcaatagag
     1081 ccaaagacat cttaagcgac ccgaaattca taaatcaaaa gggtacacga tcactgatag
     1141 atgaagatgc aagagttggt agaaaaagca agacccagga tttttatggc tacaagtcag
     1201 aatttgtgat gacaacagaa gaaaggataa taacctcagt aagaacagca aatggagcat
     1261 acatagacgg aagttacgcg aaagaactcc tggacagcac aaagaaagct ggagtaaata
     1321 ttagggaagt atatggagat aaggcatact ttagaaaatt catactggat gacatagagt
     1381 ctgtacaagc aagagcctac ataccggtta gtgcagtggt atacagactt gatgaaacaa
     1441 aatttacata caacaaagat tcagatgagt ggcagtgcat gcaggggaat acaagcatag
     1501 aaagaaaata cttcacaggt acgagccaag gaaaactcag ggcaggttac aaatactatt
     1561 ttgatatagc acagtgcgaa aaatgcccaa tgcatgatga gtgttcaggt agcgataaaa
     1621 ggagagtctt acaagttggt ttaaatacaa tgcagttcta tgagatctct caatatcaaa
     1681 agacggaaga gtttagagaa aagtataaaa agagagcatc aatagagggt aaaaatgcag
     1741 agttgaaaag atttcacgga ctgtcccgag caagagggta tggtctttta agtgtctcga
     1801 tccagtcgaa attggctgcg atagcggtaa atataaagag gatagctgca atagcatcct
     1861 ctctatattt tacaaatatc caattttggt tattttgggg tccaagatat gatttttagg
     1921 tttcagctaa aaaatgtcgc taaaaaacct tagtttttca ctggtctcgg acggttcttt
     1981 ttggctggaa ttgttaagtt aattaaacaa ttccagccaa aaagaaccgt ccccgatggc
     2041 ttaaagtgaa tatattaacc tcagtatctc cttgttctca ggtatatcct gcatggaatg
     2101 tccataatgc acgaatcgaa gcacgccttc cttgtccacc agcatctgtg ctggcatcct
     2161 gccaagcttg aacaagttga ccttttgccc atatagcttc aacacctcat ggttaggatc
     2221 cggaatgcca taaaactcca gttcattatt tttccagtac ttctcaaagc tgtctgcatt
     2281 ctccggtcct attgccacta ttattgtatc tttctccaca aacttactgt aatcctgatg
     2341 caactgcatc atatgctttt ggcaatacat tcatacaaag cctctattta gaacgataag
     2401 tacattcttc tttcccttaa aagccgaaag cttgaatata tcccctttaa aatccttcat
     2461 ctcgaaatcc ggtgcctctc tatttacttc tacacttgcc atttaatatt cccccctgta
     2521 catcctttgt ttgcatgctc cattgtatta tacccatcca gaaaatcccc aataaaaggc
     2581 cgcctgaaaa tcccaggcgg cagaaatatt atcctattga tttgatcaag tatataagta
     2641 tagcagcagc tatgaataca acagtcaaga ctttcctgat cctc
//
LOCUS       DAOSDD010000404         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13340_length_2682_cov_5.618957, whole genome shotgun sequence.
ACCESSION   DAOSDD010000404 DAOSDD010000000
VERSION     DAOSDD010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13340_length_2682_cov_5.618957"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..379)
                     /locus_tag="PK733_19650"
     CDS             complement(110..379)
                     /locus_tag="PK733_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12785.1"
                     /translation="MSRFEKHKNLKKYKKYCRRVFYLSLLLATVGVIAADHSINGLMG
                     NNGKLRIVSFGSEDSRFEICIMNYRFHPNVEFFVKLADNIKNVLK"
     gene            complement(437..2032)
                     /locus_tag="PK733_19655"
     CDS             complement(437..2032)
                     /locus_tag="PK733_19655"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOJ12786.1"
                     /translation="MNYRGSYYKNKYKYNRSLSGKHAPNRYASNRHASNRHTTGYAAQ
                     NHTLYRYVNKRSITGKSYSRRLITRAVAAVLVILLSFGAILIGIEGGNYVYKNNFLFI
                     RDVDIEKYKEVLNNSLPIIKITYNSGNIGNPISVQLKALIKSIFGFDLNSPFTILSTQ
                     SSFLHLYYNNYFPYISFANNEDNMDNNTDKSFNGDKGNNTDNAGNPGVNTDNNGTASQ
                     DDDASRGGQDREEANREPNRNNSNNSKPEDNLKGKLDENMEDLGDEGIISCYYYEGEE
                     EKLEPPKENIVSEQKIEIMNMTNFKINTSEIDKLLKEPLNIKFDRKGPKILILHTHTS
                     ESFLKNLGDLNKKGIPDWSSDPQESVVRVGHELAEQFKKKYGYDVVHNGTVHDRPNYN
                     KSYNNSYETIDKYLKSYPSIKLVLDIHRDGLSSEQPKLRVAKKIEGKDVAQIMFVVGT
                     NGNGLYHPNWKENLKLAIKLQENLNNQYPGLARHIYISNNRYNQQFSPGSLTVEIGGN
                     GNLLSECLESVKYLTKAIDEVIK"
     gene            complement(2129..2680)
                     /gene="lepB"
                     /locus_tag="PK733_19660"
     CDS             complement(2129..2680)
                     /gene="lepB"
                     /locus_tag="PK733_19660"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ12787.1"
                     /translation="MSLAENPILKEILDWVLHIAFAILIGLFIVTFIAQRTIVDGSSM
                     LPTLHHGDQLIVEKISPRIGKLNKGDIVTLYIPEYLEKGKEYVVKRIVAMENDIVEIK
                     DGKVYVNDEELKEDYINGSLTNDVNPDYSKITVPKGCVYVLGDNRLPHASLDSRSFGP
                     INAKKVMGRVLVRYYPFNNMGYI"
BASE COUNT          804 a          517 c          370 g          991 t
ORIGIN      
        1 tattttgtga gtttgatttt gcccgtagct gaatttaaag gctctaagta ctgtagcggc
       61 agcttctgga atatatttct tcaaaaactt ttaatgcatc caagatcttt tatttcagaa
      121 cattttttat attatcagca agcttaacaa aaaactctac atttggatga aatctataat
      181 tcattataca tatctcaaac cgtgagtctt cacttccaaa agaaacaatc cgtagtttac
      241 cattattgcc cataaggcca tttattgaat ggtcagctgc tataactcca accgtggcca
      301 gtaataatga caaatagaat acacggcggc aatatttttt atatttttta aggtttttgt
      361 gcttttcaaa tctgctcatg ttaacccatc acctttttat atggattttt aatataatta
      421 ttattaccat ataaaattat ttaataactt catctatagc tttagtcaaa tattttacac
      481 tttcaagaca ttcgctcaac aaattgccgt taccaccaat ttcaacagtt aaagagcccg
      541 gggaaaactg ttggttatat ctgttattac ttatatatat atgccttgct aaacccgggt
      601 actgattgtt taaattttcc tgcagtttta tcgcaagttt aagattttcc ttccaattag
      661 ggtgatagag tccattgcca ttagtaccaa caacaaacat tatctgtgct acatctttgc
      721 cttcaatttt tttggccacc cttagtttgg gctgttctga actaagcccg tctctatgta
      781 tatcgagtac cagctttatt gaaggatagc tcttaagata tttatctatt gtttcatacg
      841 aattattata tgatttgttg tagttcggac gatcatgtac cgtaccatta tgaactacat
      901 cataaccata cttcttctta aattgctctg caagttcatg gcctacacga acaacactct
      961 cctggggatc tgatgaccag tctgggatac cctttttatt taaatctccc agatttttta
     1021 gaaagctttc tgatgtgtgg gtatgaagta ttaatatttt aggtccctta cggtcaaatt
     1081 taatattcag gggctccttt agaagcttat ctatttcaga tgtatttatt ttaaagttgg
     1141 tcatgttcat aatttctatt ttttgttcac tgactatatt ttctttagga ggttcaagct
     1201 tttcctcctc cccttcataa taataacagc ttattattcc ctcatcacca aggtcttcca
     1261 tgttttcatc aagcttacct ttaagattat cttcaggttt actattattg ctattattac
     1321 ggtttggttc gcgattggct tcttccctgt cttgccctcc tcttgatgca tcatcgtcct
     1381 gagatgctgt accgttatta tcagtgttta cgcctggatt acccgcatta tcggtattat
     1441 tacctttatc tccgttaaag cttttatctg tgttgttatc catattatcc tcattgtttg
     1501 caaaagaaat atatggaaag taattattat agtataaatg gagaaaagag gactgtgtgc
     1561 ttagaatagt aaaaggactg tttaagtcaa aaccaaaaat actctttatt aatgctttta
     1621 actgcaccga tattggattt ccaatatttc cactattata tgttatttta ataataggca
     1681 gagaattatt taatacttct ttgtactttt ctatatcaac atcccgtata aacagaaaat
     1741 tatttttata tacataatta ccgccttcga ttcctataag tattgctcca aaagaaagca
     1801 gtattaccag gactgccgca acagccctgg taattaatct acgtgaataa ctcttacctg
     1861 taatactcct tttattaaca tatctgtaca atgtatgatt ttgagctgca tatccagtcg
     1921 tatgtctatt ggatgcatgt ctattggatg catatctatt aggtgcatgc ttgccagata
     1981 aactcctatt atacttatat ttatttttgt aataactacc acgataattc atatacccac
     2041 ctgctgtaaa ataatttaaa atccatacta taataattat tctacagtaa aatggattat
     2101 gacaatcaga ataattccaa tattcgcttt atatataacc catattatta aagggataat
     2161 atcttactaa aaccctgccc attacctttt tagcattaat aggaccaaaa cttctgctat
     2221 caaggcttgc gtgaggaagt ctattatctc cgagaacata tacacagcct ttgggaactg
     2281 taattttact ataatcagga tttacatcat tggtaaggct tccattaata tagtcttcct
     2341 tcaactcctc atcgttaaca taaacttttc catcttttat ttcaacaata tcattttcca
     2401 ttgcaacaat tctcttaaca acatactcct tacctttttc caggtattcc ggaatatata
     2461 atgtcacaat atcaccctta tttaatttac ctattctggg agaaattttt tcaactatca
     2521 gttgatcacc atgatgcaat gtaggtaaca tggagctgcc atccactata gttctttgag
     2581 ctataaatgt tacaataaac agtcctataa gtatagcaaa agctatatgc aacacccaat
     2641 caagaatttc ttttaaaata gggttctccg ccaaagaaat cc
//
LOCUS       DAOSDD010000405         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13475_length_2661_cov_4.842671, whole genome shotgun sequence.
ACCESSION   DAOSDD010000405 DAOSDD010000000
VERSION     DAOSDD010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13475_length_2661_cov_4.842671"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /gene="glyS"
                     /locus_tag="PK733_19665"
     CDS             complement(<1..1652)
                     /gene="glyS"
                     /locus_tag="PK733_19665"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOJ12788.1"
                     /translation="MVTNLLFEIGTEEIPAKYMPHTLKQLKAIMNTKLTENRINYNDV
                     KTFGTPRRIVVFAEGISERQSDVNELVKGPSKKVAYDSDGVPTKALSGFMRSRGINES
                     DIIIKQVDGVDYVFANKTESGKETKEILVSLLPEIIYSINFPKSMRWRDYSIRFARPI
                     RWITAILGRDVVEFSIEGVSSSNITKGHRTLSNRSIELNCADEYFDRLSGEYIMVDPE
                     ERKQSIVQQVSELARSRNGKAIIDDDLLSEVVFLVEYPTALIGNFHKEFLELPKEVVI
                     TPMKEHQRYFPVVDNDGRLLPYFITVRNGNDEFIDNVRIGNERVLHARLKDAQFFYEE
                     DLKESLESKVEKLKTVVFQERLGTVYDKAARISALAEFIGNKLSLTDAQMSMLKLAAY
                     LCKADLVTGMVNEFDELQGIMGKEYALKSGEELGVAEAIRSHYLPRYSGDEIPADTIG
                     QILSIADKLDSCVGIFGIGVQPTGSQDPYGLRRSCIGIITVIAESNMNIDISELIDYS
                     INLFGEKLTEDNSKLHSTIVDFVKQRIRVGLIDKGFRHDVIDS"
     gene            complement(1667..2545)
                     /gene="glyQ"
                     /locus_tag="PK733_19670"
     CDS             complement(1667..2545)
                     /gene="glyQ"
                     /locus_tag="PK733_19670"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOJ12789.1"
                     /translation="MYFQDVVLKLQSCWNEQGCIISNPYDVEKGAGTMNPLTFLKALG
                     PEPWHIAYVEPSRRPKDGRYGDNPNRLYQHHQFQVILKPSPDKVLELYLDSLKSIGID
                     PRKHDIRFVEDNWESPTLGAWGLGWEVWLDGMEITQFTYFQQVGSIDCKPVTSEITYG
                     LERIIMYLQEKDSVYDIDWNKDIKYGDVFHQAEYEHSKYNFEGSDTEMLYNLFDIYEK
                     EAERVINIGLVLPAYDYVLKCSHTFNLLDAKGAISVTERARYISRVRNLARIVASAYV
                     EQRQQLGYPLLKDKGC"
BASE COUNT          747 a          632 c          405 g          877 t
ORIGIN      
        1 gaatctataa catcatgtct gaaaccttta tcaataagtc ccactctgat cctctgcttt
       61 acaaagtcta caattgtgct gtgaagttta gaattatctt ctgttaattt ttctccgaaa
      121 agattaatcg aataatctat tagctcacta atatcaatat tcatattgct ttcagcaata
      181 actgtaatta tacctatgca ggaacgtctt agtccatatg ggtcctgtga gccggttggt
      241 tgtactccta taccgaatat accaacacat gaatcaagct tgtcagctat actcagtatt
      301 tggcctattg tatccgctgg tatttcatca ccggaatatc gtggtaaata gtgggaacgt
      361 attgcttccg ctacacccag ctcttctccg ctctttaaag catattcttt gcccattatt
      421 ccctgaagct catcaaactc attaaccatg ccagttacca gatccgcctt gcaaaggtat
      481 gcagcaagct ttaacattga catttgagca tctgtaagag aaagtttatt tcctataaat
      541 tcagccagtg cagatatcct ggctgcctta tcatatactg ttccaagtct ctcctggaaa
      601 acaacggtct taagcttctc caccttactt tccaggcttt ctttcaagtc ctcttcatag
      661 aaaaactgtg catctttcag tctggcatgc agaactcttt catttcctat tcttacatta
      721 tcaataaatt catcatttcc attacgtaca gtaataaaat atggcagcaa tctgccatca
      781 ttatcaacta cagggaaata ccgctggtgt tccttcattg gggttattac aacttccttt
      841 ggtaattcta agaattcttt atgaaagttt ccaattaatg cagttggata ttccacaagg
      901 aaaacaacct cgctcagcag atcatcatca atgatagctt taccattcct gctcctggca
      961 agttcactaa cctgctgtac aatggattgc tttctttctt caggatccac cattatatat
     1021 tcaccactca acctgtcaaa atactcatct gcacagttca attcaatact tctattagaa
     1081 agcgttctat gacctttagt tatatttgat gatgacactc cctcaataga gaattcaact
     1141 acatcccttc ccaatatagc tgtaatccat cttataggac gtgcaaatct aatactataa
     1201 tctctccacc tcatagactt tggaaagtta atggagtata taatttcagg aagcaggctc
     1261 accaatattt cctttgtttc ttttcctgac tcggttttat tggcaaaaac ataatcaaca
     1321 ccatctacct gcttaattat aatatcactt tcattaatac ctcggctcct catgaaaccg
     1381 ctcagcgctt tggtaggcac tccatcacta tcataagcca cctttttgga tggcccttta
     1441 acaagctcat tcacatcgct ctgtctttcg gaaattcctt cagcaaaaac tactatacgc
     1501 ctaggagttc caaaagtctt aacatcatta tagttgattc tattttcagt aagttttgta
     1561 ttcatgatag ccttcaactg cttaagagta tggggcatat atttagccgg aatctcctcg
     1621 gtacctatct caaataataa attcgtaacc atagctctac ctcctcctaa catcccttat
     1681 ctttcagcag agggtatccc agctgctgtc tttgctcaac ataagcactt gcaactattc
     1741 tggcaaggtt tcttactctg ctgatatatc ttgctctttc agtaacactg attgcgccct
     1801 ttgcatcaag gagattaaat gtgtgtgagc attttagaac ataatcatat gcaggtaaaa
     1861 ccaaaccaat gttaataact ctttcagctt ctttctcgta tatatcaaag agattgtaaa
     1921 gcatctctgt atccgagccc tcgaaattgt atttcgaatg ctcatactca gcctggtgaa
     1981 atacgtcacc atattttata tctttattcc aatctatatc atatacactg tccttttcct
     2041 gaaggtacat gattattcgt tccaggccat aggtaatttc ggatgtgaca ggcttgcagt
     2101 caatgctgcc tacctgctga aaatacgtaa attgagttat ctccattcca tccagccaca
     2161 cttcccatcc cagtccccaa gctcccaatg ttggagattc ccaattatcc tcaacaaacc
     2221 tgatatcatg tttacggggg tcaataccta tactttttaa gctgtcaagg tatagctcca
     2281 gcactttgtc aggagatggc tttaatatta cctgaaactg atgatgctga taaagcctgt
     2341 ttggattatc accatacctt ccgtcctttg gtcttcttga cggttcaaca tacgcaatat
     2401 gccatggttc cggtcctaac gcctttaaaa aggttaaagg gttcatggtt ccggcacctt
     2461 tttcaacatc gtatggattg cttataatac atccttgctc attccagcaa ctctggagtt
     2521 ttaatacaac atcctgaaag tacattatat cttcacctct caattatttt caaactaact
     2581 ggttaacaca ggtgcacctg cttaacagtg aactacaccg ctattgaaat agcgggcttc
     2641 gggttcccta tgaaattatg g
//
LOCUS       DAOSDD010000406         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13612_length_2643_cov_5.880989, whole genome shotgun sequence.
ACCESSION   DAOSDD010000406 DAOSDD010000000
VERSION     DAOSDD010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13612_length_2643_cov_5.880989"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK733_19675"
     CDS             <1..504
                     /locus_tag="PK733_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12790.1"
                     /translation="TAFICSIKIERIPAVFPKTFETVNPMWTATYTATGKDAAEKARG
                     FFLKWAKMQGFFNDSLSHRFFAYYNYERIGHKDFFYKIHVTVDEKFETDNPDIKLEEF
                     KGGYYAIMKSKYKYNGWAWGEFIDWVSKNKEYSFGNHWFFEEYKLKSPQLDMDTEMVL
                     HMPVIPK"
     gene            complement(707..1972)
                     /gene="tyrS"
                     /locus_tag="PK733_19680"
     CDS             complement(707..1972)
                     /gene="tyrS"
                     /locus_tag="PK733_19680"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ12791.1"
                     /translation="MVTVDEQIKIIEKGAEEIIGVDDLRKKLEKSQKTGIPLTVKLGL
                     DPTAPDIHLGHTVVLRKIKQYQDLGHRAVIIIGDFTGMIGDPTGKSKTRKPLTRKEVV
                     ENAKTYEKQIYKILDRDKTEVRFNSEWLSKLNFADVINLASKCTVARILERDDFQNRF
                     LAHESIGVHEFFYPLMQGYDSVELKSDVELGGTDQRFNILMGRNLQKDYGQESQVAMF
                     MPILEGTDGVEKMSKSLGNYIGINEESNDIYGKVMSIPDNLIIRYFELVTDIHPDELA
                     VIKGALDSSNVNPRDIKMRLAREITALYHGHEEAALAEEHFKMVFQRREMPENITEHE
                     ISLSMLDSDGVDAVKLLVLIGFSSSNSEARRLISQGALKVNGNKVDTLKIAGLYDGDV
                     IQAGKLKFIKIRIKGLVVGKADCSPMQPR"
     misc_binding    complement(2028..2293)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2455..>2643)
                     /locus_tag="PK733_19685"
     CDS             complement(2455..>2643)
                     /locus_tag="PK733_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12792.1"
                     /translation="AEEYPDITPAQFRNLLKSSSQDLGEPGYDIVYGYGVPDTEALKV
                     NLRLLTATITNGLNETTD"
BASE COUNT          775 a          568 c          440 g          860 t
ORIGIN      
        1 acagctttca tctgctctat taaaatagag aggattcccg ctgtgtttcc taaaactttt
       61 gaaaccgtaa atccaatgtg gacagctaca tatactgcta cagggaaaga cgctgctgaa
      121 aaagcacgag gtttcttcct taaatgggca aagatgcaag gattctttaa tgacagctta
      181 tctcacagat tttttgccta ttataattat gagaggatag ggcataaaga ttttttctat
      241 aaaattcatg ttaccgtcga tgagaaattt gaaactgata atcccgatat aaagcttgaa
      301 gagtttaaag gcggttatta cgctattatg aaatcaaagt ataaatataa cggttgggcc
      361 tggggagaat ttatagactg ggtatctaaa aacaaagaat actctttcgg caaccattgg
      421 ttttttgaag aatataagct taagagtcca cagcttgata tggataccga aatggtcctg
      481 catatgccag taataccaaa ataataaaaa agtatagtca aagacaatgc ctatttgtta
      541 tacttccatc tttataaact gaagggtcaa aaactctgga gaaagtcaaa gagacaatga
      601 gtataaaatg tttctaagta acagaaaacc gctccgacag aattttttct aattgtcttt
      661 aactgccgga gcggttaata ataatatcca tctgaatcca aaatactcac ctcggttgca
      721 taggcgagca gtcggccttg ccgacgacca gccccttgat tctgatcttt ataaacttaa
      781 gtttgccagc ttgaataaca tccccatcgt ataaaccagc tattttcaaa gtatcaactt
      841 tatttccatt cacttttaga gccccttgag aaattagtct tctagcctca ctgttacttg
      901 aagaaaaacc tatcagtacc aaaagtttta ccgcatcaac tccatcacta tcaagcatac
      961 tcagagaaat ctcatgctct gtaatatttt ccggcatttc acgcctctgg aacaccattt
     1021 tgaaatgttc ctctgctaaa gctgcctcct cgtgtccatg atataatgct gttatttctc
     1081 tggcaagtct catcttaata tcccgagggt tgacattgct tgaatccaaa gctcctttta
     1141 tcacagctaa ttcatctgga tgaatatcgg taacaagctc aaaatatctt atgattaagt
     1201 tatcaggtat ggacataact tttccatata tatcatttga ttcttcatta atacctatat
     1261 agttacccag gcttttactc attttttcaa ccccatcagt accttcgaga ataggcatga
     1321 acattgcaac ctgactttcc tgcccataat ctttttgcag gtttcttccc attaaaatat
     1381 taaacctctg atcagtgcca ccaagttcaa catctgattt aagttccact gaatcatacc
     1441 cctgcattaa aggataaaaa aactcatgaa cacctatgct ttcgtgtgct agaaaacggt
     1501 tttggaaatc atctctttcc agtattcttg caacagtaca cttggaagcc aagtttataa
     1561 catctgcaaa attaagctta gatagccatt cgctattgaa tcttacttct gtcttatccc
     1621 tatcaagaat tttataaatc tgtttttcat aggttttagc attttctaca acttccttac
     1681 gggttaacgg ctttcttgtt ttagatttac ctgtaggatc accaatcata ccggtaaaat
     1741 caccaatgat aatcactgcc ctgtgcccca aatcctggta ttgcttgatt ttacggagaa
     1801 ctaccgtgtg tccaaggtga atatcaggtg ctgttgggtc aagtccaagc ttcactgtaa
     1861 gtggtatacc tgtcttttgt gatttctcta acttcttcct taggtcatca acacctataa
     1921 tttcttcagc tcctttctca atgattttga tttgttcatc aactgtgacc ataataatac
     1981 tcctcttgct attttattaa ataaaaaact cccatcatct gaattactca gaggacgaga
     2041 gtcttattcc cgtggtacca cctcaattta ccgacacttt gcagtgtaga ccttatcaag
     2101 gactaatgct ttaaaagcac gctatccttt agcactataa cgggtgcaag attccgtcag
     2161 cagcctactt ggtttagcct ttcggtgtga agctcagaga tgtattcaga acaatgcttt
     2221 ctgcccctct cacctgtcgg gaactctctg tagaaatcag ccagttctta cttcttctca
     2281 tcaatgcttt aacaatatta cattgataac attatatatt ctgttcttga gaaaagtcaa
     2341 tcaatacatg taataaatta ggcaaaagtc tcgaatatgt ccctagccgc aatatatcat
     2401 gtatctttaa tgcaatgtcc tggacccttt ttttgcaaat tcttgttttt catgctaatc
     2461 tgtcgtttcg ttcagaccgt ttgttatggt cgctgtgagc agccgcaggt taactttaag
     2521 ggcttcagtg tccgggactc cgtacccata gacgatatca tatccgggct cgccaaggtc
     2581 ctggcttgat gattttaaca gatttctgaa ctgagccggt gttatgtcag ggtattcctc
     2641 cgc
//
LOCUS       DAOSDD010000407         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13710_length_2629_cov_3.252525, whole genome shotgun sequence.
ACCESSION   DAOSDD010000407 DAOSDD010000000
VERSION     DAOSDD010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13710_length_2629_cov_3.252525"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1889)
                     /locus_tag="PK733_19690"
     CDS             complement(363..1889)
                     /locus_tag="PK733_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ12793.1"
                     /translation="MKEMLQEVMLIQYKSLKSKIMDNSKTIRDAGPERSKNEAEVAGA
                     KRAPETIRTVGARTLRTARTAEIVRLLTAVLTAVLIAAFTLVPFAQIFSFGTVSAQNI
                     SIEKPTASDEYTEDVIKGDYIIEEYIRKNDTWYIRIEGINADRFIENLMTDNALDYIL
                     DLRDAPSSTKHIVISISDRVFDAMHQLKENLIILNPVNQIIIRPGIFSGVNCQGVNGY
                     NRLNQFYNFNYEITITLNDVISMKNIEGVKFKTQAASLKIETRKGHLFNPVSKLGNPL
                     QIIYLYNDRDWNVPGVTTGLIFDTKLSSWKKADALAEFDLDINKGFLIFEMPITGGMA
                     VADYTKDEYDNISASIYREAINNVASVHELKSIEGNIFRASKDISIEDAVKLILDVMD
                     YQYENGYLTAAIRAGIIEKNSADMQDKDCTREKALYMAIRLYELKAGEKAGPRGNAKY
                     PFEDIGKVSPQYLDNVKFAIENGFLSYIHGNMLKPDDIIMKGEFMAIIEKVLKAVGEL
                     "
     gene            complement(2109..>2629)
                     /locus_tag="PK733_19695"
     CDS             complement(2109..>2629)
                     /locus_tag="PK733_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12794.1"
                     /translation="TREYRDIPGDYPDRNSILSFISNYDVCEIFQLSGRNLFPERRVS
                     VKEAIDLYELVTDRARKGIGLNIKEKAKKLGLENIVNAGTPVKDINRQEAAGLIMKIY
                     ALKSGLDYDRLKLGGRILIKDENDIDDKFYKAVVILAENKIMRLDARGCFEPLKHITR
                     GDIMAALWKGLN"
BASE COUNT          718 a          583 c          357 g          971 t
ORIGIN      
        1 acaatatgat tattaccttt aaaatagtgt catatacatt gaaaaaaatt aatgtacggg
       61 ctgtacttgg ctcttaatca taattaagga agcgttccaa caattgtaaa attattaaaa
      121 cctgaaaaag tttatatagc atgtttaatt tcagaattcg aaaagccaga agtgaaaagt
      181 aggaagtgag aaatcgtatg ctgtattttg tgtaacaaaa atttaatgca accatgtata
      241 agttatgcaa ctctgtcact gaaaaaacgg ataaatatat gaatcacatc tacagaagca
      301 tgggaacggt tcttctgctt cccttccgcc ttccttccgc ctcgaagttc agcctccaaa
      361 cctcacaact caccgactgc cttaagcacc ttctctataa tagccataaa ctcccctttc
      421 ataattatgt catcaggctt gagcatgtta ccatgtatgt acgataaaaa accgttttca
      481 atggcaaatt taacattgtc cagatattgc ggtgaaactt tgcctatatc ttcaaacgga
      541 tatttcgcgt ttccccgagg cccggctttt tccccggctt ttaattcata caaccttatg
      601 gccatatata aggctttttc ccttgtgcag tctttatcct gcatatctgc actgtttttc
      661 tcaataattc ctgctcttat ggcagccgta aggtatccat tctcatattg gtaatccatt
      721 acatccagta taagcttaac agcatcttcg atagaaatgt ctttgcttgc ccggaatata
      781 tttccctcta tactctttaa ttcgtgaacc gaggctacat tgttaatagc ttctctatat
      841 atgcttgcac ttatattatc atactcatct tttgtatagt cggctacagc cattccgccg
      901 gtaattggca tctcaaatat aaggaatcct ttgtttatat ccaggtcaaa ttccgctaat
      961 gcatcagcct ttttccaact tgacaatttt gtatcgaaaa taagccctgt agtaactccc
     1021 ggaacattcc aatccctatc gttataaaga tatataatct gtagaggatt gccaagcttt
     1081 gaaacaggat tgaacaggtg ccctttcctg gtttcaattt taagactggc agcttgggtt
     1141 ttaaatttaa caccttctat atttttcata gaaattacat catttaatgt tattgttatt
     1201 tcatagttga aattataaaa ctggtttaat ctattatacc cattgactcc ttgacaattt
     1261 actcctgaaa aaattccagg tcttattatt atttgattaa cagggtttaa tataatcagg
     1321 ttttccttta attggtgcat tgcatcaaaa accctatcag aaattgatat aactatgtgt
     1381 tttgtgctgg aaggagcatc tcttaaatct aatatataat ccagcgcatt atccgtcatc
     1441 aaattttcta tgaacctgtc tgcattaatt ccttcaatcc ttatatacca tgtgtcattt
     1501 tttctgatat attcttctat tatataatca ccttttatga catcttcggt atattcatcc
     1561 gaggctgtcg gcttttctat cgatatattt tgtgcagata cagtaccaaa actaaaaatc
     1621 tgtgcaaaag gtaccaaagt aaaggctgca attaatactg cagtcaatac tgcagttaaa
     1681 agccttacta tttctgctgt tcttgctgtt cttaacgttc ttgctcctac tgttcttatt
     1741 gtttctggtg ctctttttgc tcctgccact tctgcttcat tttttgacct ttctggccct
     1801 gcatccctta tggtttttga gttatccatt attttacttt ttaagctttt atattgtatt
     1861 agcatcactt cttgtaacat ctccttcata tgctttgacc ccttttacca gcctaatctt
     1921 tttttgctat ttgccaacta atttcactct gttatatttc tccctattct tccttctccc
     1981 actcccaacc tctaagagtc tgcccggaaa ttacaaatta tacaagcagg ctcctaatat
     2041 ttactctgct ctccaacatt ccgatcttat ctctaacctc agctcagctc ttttcctgcc
     2101 tccaacctct agtttaaccc cttccataat gctgccatta tatctccgcg agttatatgt
     2161 ttcaaaggct caaagcatcc tcttgcgtca agtctcataa ttttattttc ggccagaatt
     2221 acaactgcct tataaaactt gtcatcgata tcattttcat ctttgattaa aattctacct
     2281 cccagtttca acctgtcata gtcaagccct gattttagag catatatttt cattattaat
     2341 cccgcagctt cctgcctgtt tatatccttt acaggagttc ctgcatttac tatgttttcc
     2401 agtcccaatt ttttagcctt ttcttttata ttcaatccaa ttcctttcct tgctctatct
     2461 gttaccagtt catataaatc tatggcttct tttacagata ctctcctttc cgggaataga
     2521 tttctgcctg ataattgaaa tatttcgcat acatcataat ttgatataaa gcttaaaata
     2581 gaattcctat cagggtaatc tccaggtata tctctatatt cccgtgttt
//
LOCUS       DAOSDD010000408         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_13770_length_2619_cov_5.803822, whole genome shotgun sequence.
ACCESSION   DAOSDD010000408 DAOSDD010000000
VERSION     DAOSDD010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_2619_cov_5.803822"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK733_19700"
     CDS             <1..520
                     /locus_tag="PK733_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOJ12795.1"
                     /translation="LNNKVWLRRNVRAFEETLLLPTHIISSSEIPGLPGYVNFNGLKV
                     VRSLDYADMSVKSMRDLSELMLAEHNGAVWALLSGAEYMPYELAPLMNVGINNLIIGS
                     EGKNEWLNASFDAIINFEIPNKGRVLVFGPESILYDNMVDSGEIFVPAGSLIEVAGNP
                     GDKFKVVVSAVD"
     gene            513..722
                     /locus_tag="PK733_19705"
     CDS             513..722
                     /locus_tag="PK733_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12796.1"
                     /translation="MISSIYTGLIGLAVENKWEDIVSSLNSVVRLSERYIADNEFPIQ
                     DLLKSYRVGIKRSNMEGVEKLYDFP"
     gene            1124..1552
                     /locus_tag="PK733_19710"
     CDS             1124..1552
                     /locus_tag="PK733_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12797.1"
                     /translation="MIEFGCLARYFTNYRDEVQFAKDNGFSFMQIWYDKKGLNLKPIE
                     ELLPVILDVAHMDNIEHLKELVSVKMPQILHVADRRLEEIHEHLPIGKGNIDFTKVFG
                     DVLTDFDGKIFFEIIQSDEDIIAAKKYFEDLLCLSQGGCL"
     gene            1549..2019
                     /locus_tag="PK733_19715"
     CDS             1549..2019
                     /locus_tag="PK733_19715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ12798.1"
                     /translation="MKYLTYNKMEKEQDQLNTNISTHEIQIDDYSEIYELWGNTSGMG
                     LSDADTHENIYKFLLRNKGLSFVCRHVDKIIGTILCGHDGRRGYIYHVTVAEKYRGRG
                     IGLMLVDKSLQALKEEGISKCHLFVFSDNVVGNAFWNAKGWTKREDIFVYSKSI"
BASE COUNT          859 a          398 c          575 g          787 t
ORIGIN      
        1 attgaataac aaagtgtggc tgcgtcgcaa tgtgagagct tttgaagaaa cactgctttt
       61 accgacacat atcatatcat cctccgaaat tcccggacta cccggttatg ttaatttcaa
      121 tggtttaaag gttgtaagat ctttagatta tgcagacatg tcagtcaaat caatgaggga
      181 tctttcggaa ttaatgctgg ctgaacataa cggcgctgta tgggccttat tatcaggagc
      241 tgaatacatg ccttacgagc tggctccact gatgaatgtc gggatcaaca acctaatcat
      301 tggaagtgaa ggcaaaaacg aatggttgaa cgcaagcttt gacgccataa taaattttga
      361 aatacccaat aaagggcgtg tccttgtatt tggaccggaa agtatactct atgataatat
      421 ggtagacagc ggagaaatat ttgttcccgc cggcagcctg attgaagttg caggcaaccc
      481 cggggataaa ttcaaggtag tggtttctgc tgttgattag tagtatctat accgggttaa
      541 ttggacttgc cgtagaaaat aaatgggagg atatcgtaag ttcactgaat tcagtggtgc
      601 gattatcaga aagatatata gccgataatg agttcccgat tcaggatctc cttaaaagct
      661 atagggtcgg cattaaaaga agcaacatgg aaggggtgga aaaattatat gacttcccct
      721 aatagaaaaa acttaaatgc ctgtccccca cgtcataccg ccagttgctt gaaaacgcca
      781 cataagaaaa ctggattccg tattaggcag aatccagtct ttaaattttt attttcttgt
      841 ctgtctactt gacagggagc agttcacatt ggaatttagt agagtgcctc gttctttttt
      901 aacttattca tttacatcaa ccgtcaagcc cgatttaaat tccacggtaa atttgtcttc
      961 gtagacggta accttttcaa tgagccgacg gacaagtatt gatttataat taaaaatgag
     1021 cacttattca cgaagaaaac tgaacacttt ctagtcgttt gaaagcatta caattttgcc
     1081 gtatatatgg ctacgaagta gaaaagaaag tagggatgaa aatatgattg agtttggctg
     1141 tctcgcacgg tattttacta attacaggga tgaagtgcaa tttgcgaaag ataatggctt
     1201 tagcttcatg cagatatggt atgacaaaaa agggttaaat ctaaagccta tagaggagct
     1261 gctcccggtg attctggatg tagctcacat ggacaacata gagcatctta aggaacttgt
     1321 ctcggttaaa atgccacaaa tactgcatgt tgctgaccgg cgactggaag aaatccatga
     1381 acatcttcct atcggaaaag gaaacatcga tttcacaaaa gtgtttggcg atgttctgac
     1441 ggactttgac ggaaaaatct tttttgaaat aatacagagt gatgaggata tcatagcggc
     1501 caaaaaatat ttcgaggact tgttgtgttt gagtcaagga ggatgtctat gaaatatctt
     1561 acatataaca aaatggaaaa ggagcaagat caattaaata caaatatatc aactcatgaa
     1621 atacagatag atgattatag tgaaatttat gagctttggg gaaatacttc tggaatgggc
     1681 ttaagtgatg ctgatacaca tgaaaatatt tataagtttc tgcttaggaa taagggatta
     1741 agctttgtct gtcggcatgt agataagata atcggaacca tactgtgtgg tcatgacggg
     1801 cgaagaggat atatttatca cgttactgta gcagagaaat ataggggaag aggcatcggc
     1861 ctaatgcttg ttgataaaag ccttcaggca ctgaaggaag aagggattag caaatgccat
     1921 cttttcgttt ttagcgataa tgtagttggt aatgccttct ggaatgcgaa aggttggact
     1981 aaaagagaag atatatttgt ttattcaaaa tccatttaag atgctgttta gtatcttttc
     2041 agaaatgata agatggtttg gtgttgtata tataattttc gcttttggat aaagcttgaa
     2101 gattttattc acaggccaat gggtgagttg accaatatta acatatgcat taatttgaaa
     2161 attgttttgc acgattgtgg agaactgtaa aatcaaaggc gagttatatt tgttttggaa
     2221 agactaccct tgcggtcctt attagcgtat ccagtataag cccgacacat gctgaaatat
     2281 gctgtaatgg tgcattattg atctatattg ggataattac ggaggaaaat aatgtgagca
     2341 caaaggtagt aaaaattata cagaaatata tagggattgt taaaagaatt gcttggagaa
     2401 agtcttaaaa taatttataa tgtagaagta cctgaagtta agagtattct cgcgatggga
     2461 aattattata atgtagtaaa tggttataaa gagttatgta tagattctac atttgcccgg
     2521 tgtcgattaa ttccaatata tgtattacgg aattccgtat tcccaagaat gctatatata
     2581 tgacgatgaa aaagaaggta ataatcttat tgtatctta
//
LOCUS       DAOSDD010000409         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14004_length_2585_cov_3.884190, whole genome shotgun sequence.
ACCESSION   DAOSDD010000409 DAOSDD010000000
VERSION     DAOSDD010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14004_length_2585_cov_3.884190"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PK733_19720"
     CDS             complement(<1..828)
                     /locus_tag="PK733_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12799.1"
                     /translation="MSQNKSKDFNVYYLNFTKVYEIAMMINNVILTKIENDRMKSFEE
                     QYGFTSSLSAQGTKNFLDGIKASISADAKETSFSSSRVVETLDVKTTKSILLRRVIEQ
                     CSNISSFEKSSEGDLVKVDNVKLELLDEESLRQFLILRRDALKGVRIEGMELNNLVSS
                     MLQDYAYILKGTVYDNVKESTIAEIIIKIPLEIQTEFENKYSINDLLIGHVSIVGIYK
                     GPVKEEFISSNTFTYFQEVGMRQKQITESSSKIIKSNTRPTYIPQLSQKEKEKEYHFL
                     "
     gene            complement(1047..1988)
                     /locus_tag="PK733_19725"
     CDS             complement(1047..1988)
                     /locus_tag="PK733_19725"
                     /inference="COORDINATES: protein motif:HMM:NF037736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12800.1"
                     /translation="MAIHALTANFNYFFARLNPSPTFVQQAAREHASIRALIEHPNGP
                     AGILSPECFLQGSYKQDTAIDTINDVDIVVMCKLWQPGSASGGGRSFDRNEIFDIIAS
                     PLKADRRYRNKVRYNSGSMCIKVDLGIKIEILPVVYRQGNYDIHDEPFRLYRPETKQW
                     EDGYARLHQSMLTLKNSAARTNGNFKPAIKVFKHLRSLCNIDAVSFHIESLLYALPDN
                     LFLGSPAEYITNILNYITNWSANNWYQTSIKTPCNDRILFSTTEWNFNSWNSFYELAK
                     LWARGANLASNSPDNNDAIEIWKIILGDNYFPANVTR"
     gene            complement(2079..>2585)
                     /locus_tag="PK733_19730"
     CDS             complement(2079..>2585)
                     /locus_tag="PK733_19730"
                     /inference="COORDINATES: protein motif:HMM:NF036719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12801.1"
                     /translation="LKFSLGEGIVNKSLSSFDEEIADKMSNMLNENTAVVPQRITSLL
                     SVSHRQKPLWKLPTGYNVLIKTVIKKADGSEYFYKNDGEPLKKHLSIYFTAITSIKKP
                     FKVYWQIVNTGDEARNCLRGGFESSDIGEMTKIEGTAYTGSHYIQCFIIKNGQCVGKS
                     KEYFVNIE"
BASE COUNT          803 a          496 c          379 g          907 t
ORIGIN      
        1 taaaaagtga tactctttct ctttctcttt ttgagataac tgtggaatat aggttgggcg
       61 agtattgctt tttattattt ttgaggaact ttcggtaatc tgcttttgtc tcataccaac
      121 ttcttggaaa taagtaaaag tgtttgaaga tataaattct tctttaacag gccctttata
      181 tatgcctaca atggagacat gtccaatgag caagtcatta atgctatatt tattttcaaa
      241 ttcagtttgg atttccagag ggattttaat gataatttct gctatagtac tttctttgac
      301 attatcgtat actgtgccct taagaatata agcataatcc tgaagcatgg aactaaccaa
      361 attatttaac tccatacctt caatacgaac accttttaaa gcatctcttc ttaaaatgag
      421 aaattgtctc agactttctt catctagaag ctccaacttg acattatcaa ctttgacaag
      481 atcaccttca gaagactttt cgaaagaaga aatattggag cattgttcaa taactctacg
      541 aaggagtatt gatttcgttg tttttacatc caaagtttca actacccttg aagatgaaaa
      601 tgatgtttct tttgcatcag cagaaataga agctttaatt ccatcaagga aattttttgt
      661 cccttgagca gaaagcgatg acgtaaaacc atattgctcc tcaaagcttt tcatacgatc
      721 attttctatt tttgttagta tcacattgtt tatcatcata gcaatttcat atactttggt
      781 aaaattaaga taataaacat taaagtcttt ggatttattt tgactcaaaa tattagctcc
      841 tttcgtagcg ttttgatcat tgtactctcc caaagtgcac attgtagcta ttacacaatg
      901 tagcctacac ttctcattat tactagaaat atccagttgc ataaaatgcc gattattagc
      961 taaacggaat atggataatc ctgccaatat aagtcaagca ttttatgacc aacattgact
     1021 actgcatttt ttttagagtg tccgtctcac cttgttacat ttgccggaaa ataattatcc
     1081 cctaaaataa ttttccatat ttctatagca tcgttattat ctggagaatt gcttgctaaa
     1141 ttagcacctc tagcccataa ttttgctagt tcataaaacg aattccagct attaaaattc
     1201 cattcggtgg tcgaaaaaag aatcctatca ttgcatggag tttttattga tgtttgatac
     1261 caattattcg cactccagtt tgttatatag ttgagtatat ttgttatata ctctgctggg
     1321 ctgcccaaaa ataaattgtc aggcaaagca tataaaaggc tttcaatatg gaatgacact
     1381 gcatcaatgt tacataaaga acgaagatgt ttaaacacct tgattgcagg tttaaaatta
     1441 ccatttgtcc ttgcagcact atttttcaac gtaagcatcg attgatgtaa ccgtgcatat
     1501 ccatcttccc attgctttgt ttcaggacgg tacaagcgaa aaggttcatc gtgaatatca
     1561 taattaccct gcctgtatac cacaggaagg atttcgatct taatacctaa atcaaccttt
     1621 atgcacatac taccactatt gtaacgtacc ttattacggt accttctatc tgcctttaag
     1681 ggtgatgcaa ttatatcaaa tatttcattt ctatcaaatg agcgtccgcc acctgaggct
     1741 gatccaggtt gccataactt gcacatgact acaatatcga catcattaat agtatctatc
     1801 gcagtatctt gtttgtatga cccctgcaaa aaacattcag gtgataaaat cccggcaggt
     1861 ccgtttgggt gctctataag agcacgtata cttgcgtgct cccttgccgc ttgctgtaca
     1921 aaagtagggc tagggttcaa ccgtgcaaaa aaataattaa agttagctgt taaagcatga
     1981 attgccatta ctcctcacac ccctaaaaac gtctaataaa atcacataaa atcatagtta
     2041 tttataatat aatgattata aaattataaa atttcaacct actcaatatt aacaaagtat
     2101 tccttgcttt ttcccacgca ttggccattt tttataataa agcactgtat ataatgactt
     2161 ccagtataag cagtcccttc aatttttgtc atttcgccaa tgtcactgct ttcaaagcct
     2221 cctcttaagc aatttctcgc ctcatcccct gtattaacta tctgccaata tactttaaac
     2281 ggctttttta tactcgttat tgcagtaaaa tatatcgata ggtgtttttt aagtggctcc
     2341 ccatcatttt tataaaaata ttcacttcca tctgcctttt ttataactgt ttttatcaaa
     2401 acattgtatc ctgttggcaa tttccataat ggtttttgac gatgagacac tgaaagtaat
     2461 gatgtaatcc tttgtggaac aacagctgta ttttcattaa gcatgtttga cattttatct
     2521 gctatttctt catcaaatga ggaaagagac ttattaacaa ttccttcacc caaagaaaat
     2581 tttaa
//
LOCUS       DAOSDD010000410         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14056_length_2576_cov_7.629512, whole genome shotgun sequence.
ACCESSION   DAOSDD010000410 DAOSDD010000000
VERSION     DAOSDD010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_2576_cov_7.629512"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..769)
                     /locus_tag="PK733_19735"
     CDS             complement(23..769)
                     /locus_tag="PK733_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12802.1"
                     /translation="MQNNEQGNTAIIEQETLRKSEQKMKIFNINKNHIQGFVAGVLVS
                     AILFTGLVASFNPSFLMGSTYTVSDLTVEDIDTVYFLKSDSMQLKEASNTTENIKHIP
                     SSTHYGFEGIDILIEGSAGEKYTLVFGASSSDLTNKMTLVGVPRNNPSVEWIPISGIK
                     IISAHEYGEFYTSTPRGATVKYKKTGVSTVIDSREFRQASGIVIQFTSIGSHDTYHIS
                     YVVLTGGYGDIKTLKVLCHNKPIDYSVITP"
     gene            complement(832..1746)
                     /locus_tag="PK733_19740"
     CDS             complement(832..1746)
                     /locus_tag="PK733_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12803.1"
                     /translation="MSDYREMDEVMNHIKPLPDSKAKEKAKAAFLLKAEEAMENEKKC
                     KPKFWKNKQLVYSFASGMAAMLILVVGMMSVLNPEFLAGTVRNTGYYEINSPYTPLKN
                     GDVISADTLQEMRASGNKFKLYMENGLALYLDSVSDGAVTLQYNLTAHAALAELLQNA
                     AGGSIITLKVESGFTGIPVKYEVDLMRGVINGLTAAEQQKWLSNLYVYCYIVDQRQLE
                     RYIETGLGDALVSDHKMLPAKIAGEVLYVNTNPGLFDTGKEVLVFTLQKIDTGSIFGS
                     KKGGAGIGSGPEGHVGDIEIEGGNIIAP"
     gene            complement(1743..2330)
                     /locus_tag="PK733_19745"
     CDS             complement(1743..2330)
                     /locus_tag="PK733_19745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ12804.1"
                     /translation="MMMRHKEGKRISLNTYDRNRLGQIFDDYYDRVYTFLYFRVKNTA
                     LTEDLTSETFLKVAEKYKTYNPEKGALSTWIFTIAMNQLRSNYRSFKGKETINLEDIE
                     NLSGGADTEVCLEANETKAAILRLLNELDERQHSIITLKYYGGLSNKEISDIINISET
                     NVSTILNRAIKKLRNILEKRDEFSDFAYKSQEGKR"
     gene            complement(2290..>2576)
                     /locus_tag="PK733_19750"
     CDS             complement(2290..>2576)
                     /locus_tag="PK733_19750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOJ12805.1"
                     /translation="FPLIMRTITTCLMHGMSTAVVGFGVRITRDNKIIRLPMILGLLA
                     LSATIHSLYNILIGTRLAIVALLMPAAHYFVGIALLSNDDETQGGEAYKS"
BASE COUNT          709 a          568 c          484 g          815 t
ORIGIN      
        1 aaggtgcggg tttgggaaaa cattagggcg ttattacact gtaatcaata ggtttattat
       61 gacaaagtac ttttaaagtt tttatgtcac catagccacc cgtaagaact acataagaga
      121 tatgatatgt atcatgactt ccaatactcg taaattgtat tacaatccca ctggcttggc
      181 gaaattctct tgaatcaata acagttgaaa ctcctgtttt tttatatttc actgtggctc
      241 ctcttggagt ggatgtataa aactcaccat attcatgagc agatatgatt ttaatacctg
      301 aaataggaat ccattccaca gaaggattat ttctagggac accgaccaat gtcattttgt
      361 tcgttaaatc tgaggaactg gcaccaaata ctaaagtgta tttttctcct gccgaaccct
      421 ctattaaaat atcaatgcct tcaaacccat aatgagtact ggaaggtata tgtttaatat
      481 tttctgtagt attacttgct tcttttaatt gcatgctatc agatttcaag aaataaacag
      541 tatctatatc ttcaactgtc aaatcagata cggtgtaggt tgaacccatc agaaaggaag
      601 gattaaaact ggcaacaaga cctgtaaaga ggattgctga aactagtacc ccggcaacaa
      661 atccttggat atggtttttg ttaatgttga atattttcat tttttgttcg gactttcgca
      721 atgtctcttg ttcaattatc gctgtatttc cctgttcatt gttctgcata ctcttctctc
      781 ctttaactct tcaattatta ttgtatagac cgcacaaaac ggatgaaatc atcaaggtgc
      841 gattatgtta cctccttcaa tctcgatatc tcctacatga ccttcgggcc cgctgccgat
      901 accggcaccg ccttttttag aaccgaatat agaacctgta tcaatctttt gaagagtaaa
      961 aaccaacacc tctttgccgg tatcaaacag gccagggttg gtatttacat aaaggacttc
     1021 cccggcaatt ttggcgggaa gcatcttatg atcacttacg agtgcatcac ccaagcctgt
     1081 ctctatatac cgctcaagtt gacgctgatc cacgatgtaa cagtaaacat agagattgga
     1141 aagccatttt tgctgttctg cggcagtcag accgtttata acaccacgca tgaggtcaac
     1201 ttcatatttg accggaattc ccgtaaaacc gctttccacc tttagtgtta taatcgatcc
     1261 gcctgctgcg ttttgaagca actctgcaag cgctgcatgg gcagttaaat tgtattgcaa
     1321 tgtaacggcg ccatcactca cactatcaag atatagagca agcccatttt ccatatatag
     1381 cttaaacttg ttgcccgaag ccctcatctc ctgaagcgta tcggcggaga tcacgtcgcc
     1441 gtttttaagc ggcgtgtaag gactgtttat ttcataatac cccgtgttgc ggactgtacc
     1501 ggcaagaaac tcaggattta gcactgacat catgcctaca accaggataa gcatagcggc
     1561 catccccgat gcaaaggaat agacgagctg tttatttttc caaaacttcg gcttgcattt
     1621 tttttcgttt tccatggcct cctcggcttt cagcagaaag gctgcttttg ccttttcttt
     1681 tgcctttgaa tcaggcagtg gcttgatatg gttcattact tcatccatct ccctgtaatc
     1741 gctcatcttt ttccctcctg actcttatac gcaaaatcag aaaattcgtc acgcttttcc
     1801 agtatatttc ttaatttctt tatagcacgg tttaatatgg tgctgacatt agtttcggat
     1861 atgttaataa tgtcgctgat ttccttattg gaaagcccgc cataatactt tagagtgatg
     1921 atgctgtgtt ggcgttcatc taattcattc agaaggcgga gtatggccgc tttggtttca
     1981 ttcgcttcaa ggcagacttc cgtatcggcg cctccactta aattttctat atcctcaaga
     2041 ttgatagttt ctttaccttt aaaggaacga tagttgctgc gcagctgatt cattgcaata
     2101 gtaaatatcc atgtgcttag agcacctttt tcaggattat atgtcttata tttttcagct
     2161 acctttagaa aagtttcgct ggtcagatcc tcggtcaaag ctgtattttt cactctgaaa
     2221 tacagaaagg tatataccct gtcataataa tcgtcaaaaa tctgcccaag tctgtttctg
     2281 tcataagtat taagacttat acgcttcccc tccttgtgtc tcatcatcat tcgacagcaa
     2341 tgcaataccg acaaaataat gggcggcggg cataagcagg gcaacgatag ccagacgtgt
     2401 gccaatcaaa atgttataca gcgaatgaat ggtcgctgac agcgcgagca ggcccaaaat
     2461 catcggaagc ctaatgattt tattgtccct ggtaattctg actccaaagc ctaccactgc
     2521 tgtggacatt ccatgcatca aacaggtggt gatggttctc ataatcagtg ggaaaa
//
LOCUS       DAOSDD010000411         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14067_length_2575_cov_3.570238, whole genome shotgun sequence.
ACCESSION   DAOSDD010000411 DAOSDD010000000
VERSION     DAOSDD010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14067_length_2575_cov_3.570238"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK733_19755"
     CDS             <1..591
                     /locus_tag="PK733_19755"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HOJ12806.1"
                     /translation="RRYIMFILYTVVISIIISYLLGGRFKFILQQPLKHIWLATFGLL
                     IQIIIFTDFFASRCNDTISVVLHGVSYILVISFIFINRKIPGILLIGIGIILNATVIF
                     LNGGYMPASLESVEKTSAGRQLEILSQGSATNNSQAITEDTILPWLADIFYIPSWVPL
                     SNVFSIGDVLIAAGVCLYLIRCMKPIDKGMETVNSK"
     gene            complement(613..975)
                     /locus_tag="PK733_19760"
     CDS             complement(613..975)
                     /locus_tag="PK733_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ12807.1"
                     /translation="MIDSCNDNCEFFEVHKDITDNVKSYMMKEEDAVKLSEVFKVLGD
                     PTRIKILYVLSKYEMCVCDIAEVLEMTQSAISHQLRVLRTMRLVKFRKEGKSMFYSLD
                     DEHILQLFNQGMEHIQHN"
     gene            1363..2172
                     /locus_tag="PK733_19765"
     CDS             1363..2172
                     /locus_tag="PK733_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ12808.1"
                     /translation="MIYLYVCLVMIIAALIYMRYEASIVKTERINFTLGKKGLKIIQL
                     SDIHIKLLRVPVQTVKKVIENENPDIILFTGDYIDKPCHIEKFLNFLIQIKSNAVIYL
                     CLGNHDYNALKYQGKNILQHFTDTLKSNGVTVLHNRAICYAKDTVKYNIIGIEDLSEG
                     KPDIDKALTLCSKEAFANIAFSHNPDVVFEIPNGKVDFLFCGHFHGGQIWMPFKLEFK
                     FLRKEILCKIGINRGLHRINGINLYINRGLGNVCFPLRFLSKPEITIFHLP"
     gene            2271..>2575
                     /locus_tag="PK733_19770"
     CDS             2271..>2575
                     /locus_tag="PK733_19770"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOJ12809.1"
                     /translation="MSQYVINLLKVAFISAIPLIEQRGAIPVGILIYKLHPFVAFTAS
                     FLGSLTPVPFVLLLFNIVLDWMKRFEFLGWFNDFIENKVRKGSAKIERYKEIGLIIFV
                     "
BASE COUNT          830 a          404 c          433 g          908 t
ORIGIN      
        1 aggagatata taatgtttat tctatatacg gttgttatta gtataattat ttcctattta
       61 ttaggcggaa ggtttaaatt tattcttcag caacccttga agcatatttg gttagctacc
      121 ttcggtcttc taatacaaat tataattttt accgattttt ttgcttcaag gtgtaatgat
      181 accatatcag tagtacttca tggagtatca tatatattag ttatttcttt tatttttatt
      241 aatagaaaaa ttccaggcat cttgctaatt ggtataggaa taatactgaa cgcaactgta
      301 atttttctta atgggggcta tatgccggca tctctggaaa gcgttgaaaa gacgtctgca
      361 ggaaggcaat tagaaatact aagtcaaggg tctgccacaa ataattccca ggcaataacc
      421 gaagacacca tacttccctg gcttgcagat attttctaca tcccatcctg ggtgccgctt
      481 tcaaatgttt tcagcattgg agatgtcctt atagctgctg gagtatgcct ataccttata
      541 agatgcatga agccaataga taaaggcatg gaaacagtaa attcaaaata gtgttgctta
      601 atatttattt catcaattat gttgtatatg ctccatgccc tggttaaaaa gctgcaaaat
      661 gtgttcgtcg tccagagaat aaaacattga tttaccttct tttctgaatt taacaagacg
      721 cattgtcctt aatactctta gctggtgaga tatggctgat tgtgtcattt caagcacttc
      781 agcaatatcg cagacacaca tttcatattt tgaaagcaca tatagtattt ttatccttgt
      841 aggatctcct aatactttaa acacttcaga aagctttaca gcatcttctt ccttcatcat
      901 atatgacttt acattgtcgg taatatcctt atgcacttca aaaaattcac agttatcatt
      961 acagctatca atcatactat acctctcaat cattccatat caattttcat tatttccttg
     1021 acactacctt aactactata tttatgattg ttcccagtaa gtcgttttac ggcaacacac
     1081 tgcaggatta tctgtagtgt gttgcatctt cgttgcaacc tggtgcaatc taatatggct
     1141 atttatagta tatttcttaa aaatcagagt gtcaatttta acagatgtaa agagagcaga
     1201 ttgtaaaaca cacagtaaat agtaaattgt aaagttgatg tttcctgata tggttggtat
     1261 aattattcag tactattatt tttgtttgtt atgctagatg caatattttt gtgatataac
     1321 attacactat tatgctttcg atgtaaattt ggaggtacat taatgatata tctttatgtt
     1381 tgtttggtaa tgataattgc tgcactaata tatatgagat atgaagcaag tattgtaaaa
     1441 acagaaagga ttaattttac ccttggcaaa aaaggtctaa aaattattca gctttcagat
     1501 atacatataa agttacttag agttccggtt caaactgtaa agaaagtaat cgaaaatgaa
     1561 aatcctgaca taatactttt caccggagat tatatcgata aaccatgcca tattgaaaag
     1621 tttcttaatt ttcttataca aattaagagc aacgcagtaa tatatttatg tcttgggaat
     1681 cacgattaca atgccttaaa gtaccagggc aaaaacattc ttcagcattt tacagataca
     1741 ctgaaaagta atggtgttac ggtactgcat aaccgggcta tatgttatgc aaaagatact
     1801 gttaaatata atatcatagg tattgaagat ttgagtgaag gaaagcctga tatcgataag
     1861 gcccttactc tttgttccaa agaagctttt gcaaacattg ctttttctca taacccggat
     1921 gtggtttttg aaatacctaa tggtaaagta gactttctct tttgcggtca ttttcatgga
     1981 ggtcagatct ggatgccctt caaattggaa ttcaagtttc tacgtaaaga gatactatgt
     2041 aaaataggta taaaccgtgg tctccacagg ataaatggaa ttaacctata tattaaccgt
     2101 gggctgggta atgtttgttt tcctttaaga tttttatcaa agccggaaat aactatcttc
     2161 catttgcctt aaaaaatcta caaaatattc tttctatttt atgtcataaa caagtgtgtt
     2221 atactaataa atattattta agaattaata tgcaaaggaa gattattaca atgagtcaat
     2281 atgttattaa cttgctaaaa gttgcattta tatctgccat acctctgatt gaacaaagag
     2341 gagcgatacc tgttggtata ctcatataca agttgcatcc gtttgtggct tttactgcaa
     2401 gctttctggg gagtcttaca ccagtaccat ttgtcttgct tttatttaat atagttttgg
     2461 actggatgaa aagattcgaa tttttaggtt ggttcaatga ttttattgaa aataaggtaa
     2521 ggaaagggtc agcaaaaata gagaggtata aggaaatagg acttataatt tttgt
//
LOCUS       DAOSDD010000412         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14099_length_2570_cov_3.523260, whole genome shotgun sequence.
ACCESSION   DAOSDD010000412 DAOSDD010000000
VERSION     DAOSDD010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14099_length_2570_cov_3.523260"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PK733_19775"
     CDS             complement(<1..1234)
                     /locus_tag="PK733_19775"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOJ12810.1"
                     /translation="MEKIPENIQVNISPDKMKAFIIISASDYNNEVPSLMELLNYLKE
                     NEVVYGINKDILNVIISQGLFNQEVLVAEGTYPVDGIGGSLKLHFNIKRDKKPAILED
                     GRVDFRNLGLIENVRKGQKLCSIIPPLPGEDGKTVTGEVIASSKGKPAKSPIGKNVEL
                     TEGLDGLIASIDGQVSYLNDRIHVYPYYEVSSDVDNSTGNINFVGNVIIRGSILSGFQ
                     VEAGGNIEIWGVVEGATVKAGGDILIRRGVQGNSKATIISEGNIVARYIEHSNIEAKN
                     DIRAEAIMHSTVKCGGKLELSGRKGLLVGGNIKVRKEIKAKVIGSQMATFTEIEVGVP
                     PELREISRSLKEEVAKLESDLKKAEQIINILLKLNTNNMLTEDKKKLLEKSLKTKQFY
                     VSRISQLKYESDVVEEQLN"
     gene            complement(1366..2127)
                     /locus_tag="PK733_19780"
     CDS             complement(1366..2127)
                     /locus_tag="PK733_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ12811.1"
                     /translation="MASSTTQFELWKKYCKTKDPEIRGELIIEYLHLVKYIAGRMNIY
                     FSTSIEYDDIISFGVFGLIDAIDKFDITKGVKFETYATLRIRGSIIDNIRELDWVPRS
                     LRQKSKELERLYLELETQNGRPPADKEIVDKMGISMGEYNKLLSDVNIQPLISLEDYL
                     ERNYKLNTNIANTKTDEMPDNHLELIELKNFLGEAIDKLPEKERTVISLYYYDELTLK
                     EIGAILKVSESRISQLHTKAILRLRGKLSKYMNDL"
     gene            complement(2174..2401)
                     /locus_tag="PK733_19785"
     CDS             complement(2174..2401)
                     /locus_tag="PK733_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12812.1"
                     /translation="MEYVKRLPFILSMFMSALIGLISYISGVAQKDIYIRISISLALF
                     YFLGLFIRMSFKNLYNSFLEKKNAAKTKLKK"
BASE COUNT          752 a          547 c          308 g          963 t
ORIGIN      
        1 tatttaattg ttcttcaact acatctgatt catactttaa ctgagaaatt ctactgacat
       61 aaaattgctt tgttttcaaa ctcttctcca ataacttttt tttatcttcc gttaacatat
      121 tattagtatt gagttttaat agtatgttga taatttgctc tgctttcttt aaatcgcttt
      181 ccaatttagc cacttcttct tttagtgatc tgcttatttc acgcagttca ggaggcactc
      241 ctacctcaat ttcagtaaaa gtcgccatct gggaaccaat tacttttgct tttatttcct
      301 ttctgacttt aatatttcct cccactagaa ggccctttcg tccggaaagc tctagtttac
      361 ctccacattt aacagtacta tgcattattg cttctgccct tatatcattt ttagcctcaa
      421 tattgctatg ctctatatac cttgcaacaa tattcccttc acttataata gtggctttgc
      481 tatttccctg tactcccctt ctaatgagaa tatctccgcc ggcttttaca gttgctccct
      541 ctacaactcc ccatatttct atgttgccgc cggcctcaac ttgaaaaccc gataatatgc
      601 tgcctctaat aataacattt cctacaaagt taatatttcc ggttgaatta tctacatctg
      661 aagaaacttc atagtatgga taaacatgta tcctgtcatt taaatagctt acctggccat
      721 caatcgacgc aattaatcca tctaatccct ctgtaagttc cacgtttttc cctattggtg
      781 actttgccgg cttcccttta cttgaagcta taacctcacc ggtaactgtt tttccatcct
      841 cccccggtaa tggaggaatt attgagcata acttttgacc tttccttacg ttctctatca
      901 aacccaaatt cctaaaatct actctgccat cttccaggat tgcaggtttt ttatcccttt
      961 taatattaaa atgcaactta agactaccgc caataccatc tactggatat gtaccttctg
     1021 ccactaatac ttcttgattg aataaaccct ggcttataat aacatttaaa atgtctttat
     1081 ttataccata gactacctcg ttttccttca aataatttaa tagctccatt aaagaaggga
     1141 cttcattatt ataatcagat gcagatataa taataaatgc tttcatttta tcaggtgaaa
     1201 tattaacttg gatattttcc ggtatttttt ccattttatc acgcttccta ttgttttatt
     1261 acttatgcat aagatgcaaa accagcatgt accataaaaa tataaaataa agtgatgcaa
     1321 atttcagcat tttaacatga atgagccttt gcaccaaaat cacctttaca aatcattcat
     1381 atatttggat aatttccccc ttaaccttaa tatagctttt gtatgaagct gtgatatacg
     1441 tgattccgaa actttaagta tagcaccaat ttcttttaat gttaactcat catagtaata
     1501 taaggaaata acagtccttt ctttttcggg aagcttgtca attgcttctc ctaaaaaatt
     1561 cttaagttca attaattcaa gatggttatc tggcatttca tcagttttcg tatttgctat
     1621 attggtattc aacttataat tgcgctcaag gtaatcttcc agtgaaatca atggttgaat
     1681 attaacatcg cttaaaagct tattatactc ccccatagat atacccattt tatccactat
     1741 ttctttatca gcaggtggcc gcccattttg tgtttccagc tcaagataca acctctccaa
     1801 ctctttgctt ttttgtctca aagatcttgg tacccaatcc aattctctta tattatcaat
     1861 tattgaacca cgtatacgca gtgtagcata agtttcaaac ttcacccctt tggtaatatc
     1921 aaacttgtct attgcatcaa tcagaccaaa tacgccaaaa ctgataatat cgtcatattc
     1981 aatacttgta ctgaagtata tattcatcct gccggcaata tattttacca gatgcaaata
     2041 ttctattatt aattcacccc taatttcagg gtcttttgtc ttacaatatt ttttccataa
     2101 ttcaaactgc gtagtgctgg aagccatttt gctcttctcc tgttaaacgt ttatctcttt
     2161 ttcattatac ctcttatttt ttgagtttag tttttgctgc attcttcttt tctaaaaatg
     2221 agttatataa atttttaaaa ctcattctga taaacaagcc taaaaaataa aataaggcga
     2281 gacttattga tattctaata tatatatctt tttgtgcaac tccggagata tagcttataa
     2341 gccctattaa agctgacata aacatactca atataaaggg aagtcttttt acatattcca
     2401 taatttccac ctgtatctcc tagtatgata tccatattta actcacgaaa ctttcctttc
     2461 gcagcaatta catttctttc cctattctca ttataactga agaaccaata aatgcaaaca
     2521 aatgtatgta tcgaggtgca tttttaaatt tgaagaaata tttaccagtg
//
LOCUS       DAOSDD010000413         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14363_length_2531_cov_3.664782, whole genome shotgun sequence.
ACCESSION   DAOSDD010000413 DAOSDD010000000
VERSION     DAOSDD010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14363_length_2531_cov_3.664782"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..792)
                     /locus_tag="PK733_19790"
     CDS             complement(70..792)
                     /locus_tag="PK733_19790"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ12813.1"
                     /translation="MNAEKTSFGRNIALTIVCLILGIMVAWQYKSINYNESIMSYENR
                     REEALKEDLIKLQKSNADLRVMLQKLQEDVRLYETAQAGSDESYRKLLDELEIVRIFA
                     GKVDVKGKGVIITLEDMFYTVIGSDILDVVNELRAAGAQAISVGDAVNDERIAAMTEI
                     RDASPYIMINGTQITAPVTIKAIGDPEQMEHSLKMINGVFDKLQEYLEITIKQSDEVF
                     IPKIRDDGSVIKTNLLTPVTVK"
     gene            complement(789..1511)
                     /locus_tag="PK733_19795"
     CDS             complement(789..1511)
                     /locus_tag="PK733_19795"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ12814.1"
                     /translation="MNKGSKFVFFIIYFSLGLIMTLQYRSILYSNKEKSASVLNVDRL
                     IVQLEQEKKIEDELKAKVEEYNREKEEYLKRYIEMNDDAKLKVLWENLNTLKHISGLT
                     DVEGPGIIIKLDDAPARKTDKPSHLIIHDNDIRIILNELKKAGAQAISINGERIVSVS
                     EQVCAGPTIRINKERYSVPYTINVIGPPDQLYNTLIESSRINTMLREEIKISINKSGR
                     VLVPKYRKEPVRAVNNLEVVEK"
     gene            complement(1747..>2531)
                     /locus_tag="PK733_19800"
     CDS             complement(1747..>2531)
                     /locus_tag="PK733_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ12815.1"
                     /translation="IKALLVKLGCKVELLDLKLINIKTKKTSMTSGDVNFFTSYVKMG
                     KFDLGVSIEDFSEKMMLVDDKGRIITEDMYMALISIMLFRTVKGGTVVVPISASHIVE
                     KIAADNNGKVIRTKTSTQDIMYKLMGNGETEGMLDQFTLHFDAIAGLVKILDFMSANN
                     YKLSDIVDMIPTFHMNKREVECPWNAKGKVIRQIIQEQSGNTIETMEGVKIYNENGWV
                     LILPDAELPVCRVISESFSAEFAEELSSIYADKIKEISRKKI"
BASE COUNT          698 a          550 c          331 g          952 t
ORIGIN      
        1 tatttgaaga aacattggta aatatttctt caaatttaaa aaacgcactc ccaaagtaaa
       61 tattattttc tatttaacag ttacaggagt taaaagattt gtctttatta cggaaccatc
      121 atccctgatt ttagggataa aaacttcatc agactgctta atggtaattt ctaaatattc
      181 ctggagttta tcaaatactc cgtttatcat tttcagggaa tgctccattt gttcagggtc
      241 tcctatggct tttattgtaa caggtgcagt tatctgtgta ccattaatca ttatataagg
      301 tgatgcatct cttatttctg tcattgctgc aatcctctca tcatttacag cgtcgcctac
      361 agatatggcc tgagcccctg cagctcttaa ttcatttaca acatcaagta tatcactgcc
      421 tattaccgtg taaaacatgt cttcgagggt tattataacc ccctttcctt taacatcgac
      481 cttccctgca aaaattctta caatttccaa ttcatctaat aatttcctgt aggattcatc
      541 acttcctgcc tgtgccgttt catacaacct gacatcctcc tgcaactttt gcagcatgac
      601 ccttaaatcg gcattgctct tttgaagctt tataagatcc tcttttaatg cctcttctct
      661 tctattttca tagctcataa tactttcatt ataatttatg cttttatact gccatgctac
      721 cattataccc aatatcaaac atacaattgt aagtgcaata tttctaccaa agctcgtttt
      781 ttcagcattc atttttctac cacctcaaga ttattaactg ctctgactgg ttctttccta
      841 tatttgggga ctaataccct acctgactta tttattgaaa tttttatttc ttccctgagc
      901 attgtattaa ttctgctact ttcgatcaga gtattgtata actggtcggg aggaccgata
      961 acattaattg tataaggtac agaataccgt tctttattta tccggattgt aggaccggca
     1021 caaacctgtt cactgactga tactatcctt tctccattta ttgatattgc ctgtgcccct
     1081 gcttttttca gttcattaag tattatcctt atatcattat catgtattat caaatggctt
     1141 ggcttatcgg tttttcttgc aggagcatca tcaagtttaa taattattcc cggaccttct
     1201 acatccgtta atccggaaat atgttttaat gtattaaggt tttcccataa aaccttcaat
     1261 ttggcatcat cattcatttc tatatatctt tttaaatatt cctctttttc cctgttatat
     1321 tcctctacct ttgctttaag ctcatcctct attttctttt cctgctccaa ttgaactatt
     1381 aacctgtcca cattcagaac agaagcagat ttttccttat tgctatataa aatactcctg
     1441 tattggagcg tcattatcaa acccagcgaa aaatatatta taaaaaacac aaatttactg
     1501 cccttgttca tatgaatact cctgtttctg aattctactc ctttctatat accaatttta
     1561 cttattatat tcgtattttg caaggggaaa gttagagatg gacacatttt ctgtatatgg
     1621 taaaaaatat agcactatta tggcattatt atggcattat tcggttagca aaagcaaaaa
     1681 attacttacc cctcctccaa attttgcctt ttgccgcctg ctatcgcaat aggcacggct
     1741 tatctctcat atcttctttc tgcttatttc cttgatttta tctgcataaa tactgcttaa
     1801 ttcttctgcg aactctgcag aaaagctttc acttattact ctgcatacgg ggagttcggc
     1861 atcaggcaga ataagaaccc aaccattttc attatatatt ttaacccctt ccatcgtctc
     1921 aattgtgttt ccggactgct cctggataat ttgccgtata acctttccct ttgcattcca
     1981 tggacactct acttcccttt tattcatatg aaaagttggg atcatatcca caatatccga
     2041 aagcttatag ttatttgcag acatgaaatc aagaattttg acaagtccgg ctatggcatc
     2101 aaaatgaagt gtaaattgat ccagcattcc ttctgtttca ccgtttccca ttagtttata
     2161 cattatatcc tgagtggaag ttttagttct tataaccttt ccattattat ctgccgctat
     2221 tttttctaca atatggctgg ctgatatggg aacgactaca gttcctcctt taacagtcct
     2281 gaagagcatt attgatataa gtgccatata catgtcttct gttattatcc tgcccttatc
     2341 atctacaagc atcatctttt ctgaaaagtc ttctatagaa acacccaaat caaatttgcc
     2401 catttttaca taacttgtaa aaaagtttac atcgcctgat gtcatacttg ttttttttgt
     2461 ctttatattt ataagtttca aatccagcaa ctctacctta caacccaatt ttaccagtaa
     2521 agctttaata a
//
LOCUS       DAOSDD010000414         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14409_length_2526_cov_5.892756, whole genome shotgun sequence.
ACCESSION   DAOSDD010000414 DAOSDD010000000
VERSION     DAOSDD010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14409_length_2526_cov_5.892756"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PK733_19805"
     CDS             complement(<1..1001)
                     /locus_tag="PK733_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12816.1"
                     /translation="MAVVILFWQSKPMRSIVSLCNLANVNISKTSDTFGIICRTLTGL
                     MSTIETHNQKLSRIMPVLRDNFVLWLLSEMPADIDEIQDNMLLMQINFPYKNYCVLAV
                     KAEIISKDSDDYVDEFIHEYAVAEVKLRLENIFNNDNALCHFYKKDSLILGFVNFNYD
                     AEYLNATCEELTKCPIYGYDIYLCSGHVVQNINDIANAAKYTINGLKWSYIYPEKKYF
                     SSVETCRFEKTPALGIQPILKGFISWLKLEDYDRCLHELDNFVEKVRMDGCRIDDVHS
                     LIYSIAHEIEGEGGIDAKFRHDFMEIYSQNTDILNFRDALYKRICNLKQVSSKFADNT
                     "
     gene            complement(1091..1936)
                     /locus_tag="PK733_19810"
     CDS             complement(1091..1936)
                     /locus_tag="PK733_19810"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ12817.1"
                     /translation="MKNFHKLRGFIMYQFLASFVIVLICTLISSAVSYQVLREEMSQW
                     LIDNNNKVLEQYSETIDTLIASNSNEVYKQILNDISMVESMKYYLYFPLKDNIMDTLK
                     INKYLSSIKESNQLIQSVGIFYVDNNLLISSDKIRYDLFYEYRRKELQLYSDVLNASS
                     KRKNSFYNIADQSTLCMMRPIVSGGKINALFVINYSLGGLRERLRNVLPSDFGNFFVV
                     DSDNTVIFDIDGVNAAENLFNNSVYARVFSEPYGVNTYSVEIEGEPSIVSYKNYGEGS
                     GNLYQ"
BASE COUNT          831 a          458 c          355 g          882 t
ORIGIN      
        1 gtattatcag caaatttact gctcacttgc tttagattgc atattcgttt ataaagagca
       61 tctcgaaaat ttaaaatgtc tgtgttctga gaatagattt ccatgaaatc atgtctaaat
      121 ttcgcatcta taccgccttc accttcaatt tcatgggcaa tactatatat taagctatgt
      181 acatcatcaa tcctgcagcc gtccatacga actttttcta caaaattatc taattcatgc
      241 aggcagcgat cgtaatcttc aagtttaagc caggatatga agccttttaa tataggctga
      301 ataccaagtg caggggtttt ctcgaacctg caagtttcta cactgctgaa gtacttcttt
      361 tcagggtaaa tatagctcca ttttaacccg ttaatagtat attttgcagc gttcgcaata
      421 tcattaatgt tttgaaccac atgaccagaa cataaataga tatcataacc ataaatagga
      481 catttggtaa gctcttcaca tgttgcattc aagtactccg catcataatt gaagtttaca
      541 aaccccaata taagcgaatc ctttttataa aagtggcata aggcattatc attattaaaa
      601 atattttcca aacgaagctt tacttcagct acggcatatt catgtataaa ctcatctaca
      661 taatcatcag aatcctttga aataatttca gccttgactg caagaacgca ataattttta
      721 tatgggaagt ttatctgcat aagcagcata ttatcctgta tttcatctat gtcagcaggc
      781 atttcagaaa gcagccataa cacgaaatta tcacgaagga cgggcattat acgggaaagt
      841 ttctggttgt gtgtctctat tgtactcatt agccctgtta atgttctgca aataataccg
      901 aatgtatcac tagttttact aatattaacg ttagccaaat tgcacaaaga tactatggaa
      961 cgcatcggtt ttgactgcca aaataggata acaaccgcca atataaatcc tgccaatatt
     1021 gtgattatta cagaaataag caaattttta agaatataga ttatcggttt catataaata
     1081 ttggtcggtg ctactgatat aaatttccac ttccttctcc atagttttta tatgagacaa
     1141 tgctaggttc tccttctatt tctacactat aggtgttaac tccgtatggc tcgctgaata
     1201 cccgtgcata tacagaatta ttaaatagat tctctgcagc gtttacacca tcaatatcga
     1261 atataactgt attatcagaa tcaactacaa aaaaattacc aaaatcagaa ggaagtacat
     1321 ttcttagtcg ttctcttagt ccacctaaag agtaatttat aacgaatagg gcgttaattt
     1381 tgccacccga aactatagga cgcatcatgc ataaggtgct ttgatctgct atattgtaaa
     1441 atgagttctt ccgtttcgaa gaggcattta gcacatctga atataattgc agttctttcc
     1501 tgcgatattc atagaacaaa tcgtaacgaa ttttatcact ggaaatcagc aaattattgt
     1561 ctacatagaa tatacctact gattgtataa gttgattaga ttctttaatg cttgataaat
     1621 atttattgat ctttagagtg tccataatat tatccttcaa gggaaaatat aaataatact
     1681 tcatgctttc aaccattgat atatcattga gtatctgctt gtaaacctcg ttggaattag
     1741 atgcaataag tgtgtcaatt gtctctgaat actgctcaag taccttatta ttgttgtcaa
     1801 ttaaccactg agacatttct tcccgcaaga cctgatagga tacagcactg gaaatcaaag
     1861 tgcaaataag cacaattaca aaagaagcta ggaactgata cattataaaa cctcgaagtt
     1921 tgtggaagtt tttcatagta tacacacctc tcgcttttat ccgggcactc catcgtatgt
     1981 atagatttta catttacaaa cctaattttt caatagtttt attaattaac tttgaatata
     2041 tatgtaagca ttatagactt atgcacatca tccattactt gtaatttttg tatttacaca
     2101 acttcctatc ttctcttttg aaaaaacttt tattccatta ttaaaaagtg gtagtatggt
     2161 ctatgtaaac ttcatttgct tatttaaaca acctttactt ttgttaaatt actatgtctg
     2221 gaaaactttc catgtttacc ttatatctat cctatataat ttaagtttct cagttttctg
     2281 ttaatgattt caaattatta tataaatttt agcgcaaaga ctcatttacc atcgaaatac
     2341 tggaattctt attatttcaa tcttacagtt tatatgaaga cttcaacaat atatttctaa
     2401 gctaaattcg tcttttgcac tataatctga atttgtgtag caaaaaatct tatgttatat
     2461 atttacacaa gcttaaaaca aaatatcaat ataactctct actttgtaca caagtttccg
     2521 tacagt
//
LOCUS       DAOSDD010000415         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_14981_length_2452_cov_7.610763, whole genome shotgun sequence.
ACCESSION   DAOSDD010000415 DAOSDD010000000
VERSION     DAOSDD010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14981_length_2452_cov_7.610763"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK733_19815"
     CDS             <1..714
                     /locus_tag="PK733_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ12818.1"
                     /translation="PEDVGVDGNIDIKANTYHIRARFICHTFSPNEEGVRISGDTYYS
                     PYSNVFSIGTDAYQKYKNASIWAVEELDKAAEYGFITDKIKGKMNGPITREEFCEVVI
                     KLYEKMMGKAATFSNMDAFIDTKNPEIFKAYELGIVKGVGGNKFAPNELTNREQVAVM
                     MHRSVKVLKPDADFSIAGSEKFSDENIISSWALESVKFMNKNGLIKGVGDGRVDPKGT
                     TTREQAVIMVVRTYEKYGK"
     gene            740..1660
                     /locus_tag="PK733_19820"
     CDS             740..1660
                     /locus_tag="PK733_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12819.1"
                     /translation="MGDNQKTDLIGNKGFRIFVILTIVAFIFSFTACGKKEKNIDKGD
                     ADKVKVLEKVAGDKDKPGKAADYKDKATKAEPALNLNMGDVGNNLPLPDNYPKEILPL
                     AGDAKIINVVQNTQNNGLGIIYISDDDLDSLLELNESALSDAKDLKVLETDDGYMLMA
                     SLEGVHYTIMIGEGLFDQNSEYKGKQSVYIELTRAEGLSDNGEGAEIPADGGKPWPAS
                     DMAEVPELPGYIGNVHIGDDAIRLEITVEGLDVVKGYIGELKNAGFSFDSEPDTSNEK
                     IEFIAFKDSTMLNFAYKAAEKLVFIEYIKS"
     gene            1674..>2452
                     /locus_tag="PK733_19825"
     CDS             1674..>2452
                     /locus_tag="PK733_19825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ12820.1"
                     /translation="MDIMNIINMINMMNIMDIMDGVYMKGLIAGILVVVMLFITGCQG
                     KDNDQGKTKSSVNEPTSFSAKDVDKKKVLGLCPSVMDIARKGFTPEAMKGMEEFVKAI
                     DEAEGNAKKLTALMLATDISLGQLGMVNQDILLYMAGKALSAYPEHPVILNNAASVLF
                     DSEKTDTAIALYMEAIKAAPRNPIFLTNLANAYIQKGDFVSAEKCARQSLLADPDYSA
                     AYQVLTTCHLEKGNDMLAAETMIKSTKNCFNDVSISQFTSFL"
BASE COUNT          831 a          383 c          575 g          663 t
ORIGIN      
        1 cctgaagatg tgggagtaga tggaaatatt gatataaaag caaacacata ccatataaga
       61 gccagattca tttgccatac tttttctcca aatgaggagg gcgtcagaat ctcaggagat
      121 acttactact ctccatactc gaatgttttc tctattggca cagatgcata ccaaaagtac
      181 aaaaatgcca gcatctgggc agtggaagag ttagataaag cggcagaata cggatttatt
      241 acggataaga tcaagggtaa aatgaatgga ccaattacac gcgaggaatt ctgtgaggtt
      301 gtaataaaac tttacgaaaa aatgatgggt aaagctgcaa catttagtaa tatggatgct
      361 tttatcgata ccaaaaaccc ggaaatattt aaagcctatg agttaggcat tgtaaaggga
      421 gtgggcggaa acaaattcgc tccaaacgaa ttgacaaata gagaacaggt tgctgttatg
      481 atgcatcggt cggttaaagt gctgaaacca gatgcagatt ttagcatcgc tggttcagaa
      541 aaattctctg atgaaaatat catatcatca tgggcattgg aatctgtaaa gtttatgaac
      601 aaaaatgggc tgataaaggg tgtgggtgat ggaagagtcg atcccaaagg cacgactacc
      661 agagagcagg cggtaattat ggtggtcaga acctatgaaa agtatggaaa ataagatttt
      721 ataaatgggg gattagttca tgggtgacaa tcaaaaaaca gatctaattg ggaataaagg
      781 gttcagaatt tttgttatct tgacaattgt ggcttttata ttctctttta ccgcttgtgg
      841 caagaaagaa aagaatatag acaaaggaga tgccgacaaa gtgaaagtgc ttgaaaaagt
      901 agcaggtgac aaggataaac ccgggaaagc agcagattat aaagataagg ccacaaaagc
      961 tgaaccggca ttaaacctaa atatgggaga tgtgggtaat aatttgccgt tacctgataa
     1021 ttatccaaaa gaaatattac ctcttgcagg ggatgcaaag attattaatg tagtgcagaa
     1081 tacccaaaat aatggtctag gtattatata tataagcgac gatgaccttg acagccttct
     1141 agagctgaat gaatcagctt tgagtgatgc taaagactta aaggttttgg aaacagatga
     1201 tggttacatg ttaatggcta gtttggaagg tgttcattat actattatga ttggagaagg
     1261 tctatttgac caaaactctg agtataaggg caagcaaagt gtttatattg agctcaccag
     1321 agcagaaggg ctatcagata atggggaagg ggctgaaatt cctgcggatg gcggtaaacc
     1381 ctggcctgca tcagacatgg cggaagttcc tgaattgccg ggatatatcg gcaatgttca
     1441 tattggggat gatgctataa gacttgaaat aactgtagaa ggtttggatg tggtaaaagg
     1501 ctatattggc gagttaaaaa atgccggctt tagttttgac tccgagcctg acacaagcaa
     1561 tgagaagata gaattcattg ccttcaagga cagcactatg ttgaattttg cttataaagc
     1621 tgcggaaaag cttgttttca tcgagtatat aaaatcataa tgcataatga ataatggata
     1681 ttatgaatat tataaatatg attaatatga tgaatataat ggatataatg gatggtgtat
     1741 atatgaaggg acttattgcc ggtatattgg tggttgttat gctgtttatc acaggctgtc
     1801 agggaaaaga caatgaccag gggaaaacaa agagcagcgt gaatgaacca acatccttct
     1861 cagcaaaaga tgttgataag aaaaaggtct taggcttatg tccttccgtc atggatattg
     1921 cccggaaagg atttactcct gaagcaatga aggggatgga agaatttgta aaggcaattg
     1981 atgaggctga aggcaatgcc aaaaagctta ctgcacttat gcttgcaact gatatctcat
     2041 tgggtcagtt gggtatggta aatcaggata ttcttctata tatggcagga aaagcattgt
     2101 ccgcatatcc ggagcaccct gttatactta ataatgctgc atcagttcta tttgactcgg
     2161 aaaaaacaga tactgccatt gctctttata tggaggcgat aaaagcagct ccccggaatc
     2221 cgattttttt gactaatctg gctaatgcat atatccaaaa gggggatttc gtctccgcag
     2281 agaaatgcgc aagacaatct ctactagcag atccggacta ttccgctgca tatcaggttc
     2341 tcaccacttg tcatcttgaa aagggcaatg atatgttagc cgccgaaact atgataaaaa
     2401 gcacaaaaaa ttgctttaac gatgtgagta taagccaatt cacatcgttt ct
//
LOCUS       DAOSDD010000416         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15237_length_2419_cov_5.971235, whole genome shotgun sequence.
ACCESSION   DAOSDD010000416 DAOSDD010000000
VERSION     DAOSDD010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15237_length_2419_cov_5.971235"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..970
                     /locus_tag="PK733_19830"
     CDS             74..970
                     /locus_tag="PK733_19830"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12821.1"
                     /translation="MNEIKVAIVGLDTSHSVEFARRMNDPNCDPKIKVNGLKAVSCMR
                     FETPFQDKNGLDARQKQLEGWGVKVTESFDECVADCDAIMLEINDPAYHLEYFEKAIK
                     LGKPVYLDKPMADTYASGKAIYEMGKKNNIKVFSASSLRFVTAVVEAKKELNNPEFVS
                     VYGALGDAPAGSSVVWYGVHTMEMLQKLMGPGAVSLKAVQDDKGVVCFVKYKNGSRGI
                     AELNNGSYIYGGAVRTKEKAFHYTVDMSMAYTLELIEIAKFFNGEEPSASFEDTLEVM
                     DLLDTAQRSLDSDKEEKLNGSY"
     gene            1689..2336
                     /locus_tag="PK733_19835"
     CDS             1689..2336
                     /locus_tag="PK733_19835"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ12822.1"
                     /translation="MKNNIKGIIFDMDNTLLSSKINFAAMKKETYEFVINHGILPDDL
                     DLINQTTAIIIEKAVKTKMMSQEIIKAVWEIPKKYEVMGMENAVLEPGVTQLLSELYG
                     KYYMAVVTNNSIEAAVKAFRENKIYSYFDILVGREMIKSLKPSPDGFLYILDHYNTTS
                     TDEWLSIGDAWLDGKASMDAGIRFISYRGDIQKMNTMGVYPYAVINDIRELSNFI"
BASE COUNT          838 a          354 c          475 g          752 t
ORIGIN      
        1 tcgaaaggaa aatataccgt agtttggggc ttctaagccc ctgaccacga gtttttaaaa
       61 ggagggttat agcatgaatg aaataaaagt tgcaatagta ggacttgata caagtcattc
      121 tgttgaattt gcaagaagaa tgaatgatcc gaattgtgat ccaaagatta aagtaaacgg
      181 gctaaaagca gtatcctgta tgagatttga gactccattc caggataaaa acggtttgga
      241 tgctcgtcaa aaacagttgg aaggctgggg agttaaggta actgaaagct ttgatgaatg
      301 tgttgctgat tgtgatgcaa ttatgcttga aatcaatgac cctgcatatc atctggaata
      361 cttcgaaaaa gcaataaagc ttggtaaacc tgtctatttg gacaaaccta tggctgatac
      421 ttatgcctca ggaaaagcga tttacgaaat gggcaaaaaa aataatataa aagtattttc
      481 tgcatcatca ttacgctttg tgactgctgt tgttgaagcc aaaaaagagt taaacaatcc
      541 tgaatttgta tctgtatatg gtgctctcgg tgatgcacct gcaggtagta gtgttgtatg
      601 gtacggggta catacaatgg aaatgcttca aaaactaatg ggccctggag ctgtaagctt
      661 aaaagctgtt caggatgaca aaggcgttgt ttgctttgta aaatacaaaa atggcagcag
      721 aggtattgca gaattaaata acggatctta catttacgga ggagctgtta gaacaaaaga
      781 gaaagctttt cattatacag tagatatgag tatggcatat acactggaat taatagaaat
      841 tgcaaaattc tttaatggtg aagaaccatc ggcttctttc gaagatacac tggaagttat
      901 ggatcttctg gatacagctc aacgctcttt agatagcgat aaggaagaga aattaaacgg
      961 ttcatattag ggtagggata aagtaaaaga aatccggtta atttttgagg taaccggatt
     1021 tcttttttga ttcaattgtg ctcctttaca tatacttggt gcacattttt aatttatgaa
     1081 gaaatatagt atgcatgaat cattaacttt ttacgtcaga ccatgcagaa cgcccctttt
     1141 ttaaaatggg agatgaatgc atgactacct tttttccaaa tctatcgctg ccaatctaaa
     1201 ataaagtatg tcaaaactat cttccccata attgcactga actgcagata ttctcaatgg
     1261 tttttcccac catttcatta tagtcaccct ttcatataat atttttccta tgtgctatac
     1321 tatttacctc aaaagttagc agaaaactaa ttttaatggt tttatgacaa taataaaact
     1381 caatttagtt tgttgtggaa gtgtaatcat taattccgct tgataaattt actttgtcaa
     1441 ctgaggtata aatttaatta tatagaagta tttgaataaa taatgatact tcttgttttt
     1501 caattccggc ggatatgata taatatcctc ataaatgagg atattatatg cctccggcgg
     1561 aattttgtgc atgcgaatat ttttgctccg caaaaaacgc acatgcacaa ttatatcatg
     1621 caatttcttc gaaattcatg acataataaa ccagaaagtc taagaggaag gagcataggt
     1681 catcagatat gaagaataat attaaaggca ttatatttga catggacaat actttactaa
     1741 gttcaaaaat taattttgca gctatgaaaa aggagacata tgagtttgta ataaatcatg
     1801 gaatacttcc tgatgatttg gatcttatta accaaacaac agcaattatt atagaaaaag
     1861 cagtaaaaac aaaaatgatg agccaggaaa taataaaagc agtatgggaa attcctaaga
     1921 aatatgaagt aatggggatg gaaaacgctg ttcttgaacc cggagttaca caattactta
     1981 gcgaattata tggtaagtat tatatggctg ttgtcactaa taactccatt gaagcagcag
     2041 ttaaggcgtt tagagagaat aaaatatata gttattttga tattttagtg ggaagagaga
     2101 tgattaaatc cttaaagcct tcacctgatg gttttttata tatattggat cattataata
     2161 ctacatctac tgatgaatgg ctgtcaattg gagatgcctg gctggacggg aaggcttcta
     2221 tggatgctgg aataagattc atctcatatc gtggtgatat tcaaaagatg aatactatgg
     2281 gtgtctaccc atatgctgtg attaatgata ttagggagtt aagcaatttt atttgataag
     2341 ttttgctaat aaaacttaac cgcccataat gaatagcaaa ttatccgaag tcagtggcat
     2401 aaaaaaagag ctctgtacg
//
LOCUS       DAOSDD010000417         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15387_length_2401_cov_4.988917, whole genome shotgun sequence.
ACCESSION   DAOSDD010000417 DAOSDD010000000
VERSION     DAOSDD010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15387_length_2401_cov_4.988917"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1067)
                     /locus_tag="PK733_19840"
     CDS             complement(195..1067)
                     /locus_tag="PK733_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ12823.1"
                     /translation="MKQLLILSGKGGTGKTTIASAFIKLSNARAYADCDVDAPNLHLI
                     TGQSSEPKKTDYYGLPKAEINPELCIQCDQCRQNCRFDAISVEKTYKADPFACEGCGV
                     CEAVCPVAAISLQPAIAGELTLYSNVEKVFSTAQLKMGSGTSGKLVAEVKKQMKLAAV
                     DVELAIIDGSPGIGCPVIASLSGVDMVLIVAEPSISGISDMERIIKTAEKFGIKIAIC
                     VNKFDTNLVNTEKIEFFCKGQELPFVGKVPFDSDAVKAINNGKTIVDINCASGIAVKE
                     IYNKVMKLLFGGEA"
     gene            complement(1045..1905)
                     /locus_tag="PK733_19845"
     CDS             complement(1045..1905)
                     /locus_tag="PK733_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ12824.1"
                     /translation="MRIAVLSGKGGTGKTLVSVNLAAEAKTSSYIDCDVEEPNGHLFF
                     KPDDVQEEEISVRIPRVDDKLCNGCRKCVDFCKFNALAYIKEKLIIFGEVCHSCGGCV
                     ILCPEKALIEKEKVIGKVQKGISDQVMVYTGILNTGEASGIPIIKKLLAENKRDSNKL
                     TFIDCPPGSACIVMESIKDADYCILVAEPTLFGVHNLNMVYELVKLFNKPLGVVLNKC
                     LEGENPAEKFCLERNIKILGRIPFDNELGMLNSNAEIAARESKKYNALFSSLLETVKK
                     EVQHETTTNP"
     gene            complement(1907..2275)
                     /locus_tag="PK733_19850"
     CDS             complement(1907..2275)
                     /locus_tag="PK733_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ12825.1"
                     /translation="MKIAIPVDEKSLKSNVCVSFGRTPYFLIYDIDTKESTFLDNSAA
                     ASTGGAGIKAAQTIVDNKVNALLTPRCGQNAADVLGAADIKIYKTTNTSVKENIDDYI
                     AGRLSLLDEIHAGFHGHGGN"
BASE COUNT          645 a          546 c          393 g          817 t
ORIGIN      
        1 ataattgtta tacctttatc ctctgcttta taccggagca ggtgctctat ccttgttagg
       61 ggcatatgcc cctgtcatca ggttgatttt atttaaaata atctgaccat tagatgataa
      121 cactccttct ttttctgcta tcgcaggctg ctgatatttc ccgaacaatc ggtaattttg
      181 caagaacctt taagttatgc ttctccgcca aacagcagct tcataacctt attatagatc
      241 tcttttactg ctattccgga tgcacaattt atatctacga tagttttccc gttattgata
      301 gcttttactg catccgaatc aaaaggcact ttgccgacaa agggcagttc ttgcccttta
      361 caaaaaaact caattttttc tgtatttaca aggttggtat caaatttatt tacacaaatt
      421 gctattttga ttccgaattt ttcagctgtt tttatgattc gctccatatc actaataccc
      481 gatatggaag gttcagccac gatcaaaacc atatctactc cgctaagaga cgctatgaca
      541 gggcagccta ttccaggaga accgtcaata attgcaagtt caacatcaac tgctgctaac
      601 ttcatctgtt tttttacctc tgcaaccaat tttccggaag tgccgcttcc catttttagc
      661 tgagctgttg aaaagacttt ttccacattg gaatacagcg tcaattctcc ggctatggct
      721 ggctgtaatg atatagcggc tacaggacat acggcttcgc aaaccccaca tccttcacat
      781 gcaaaaggat cagctttgta tgtcttctct actgaaatag catcaaatct gcaattttgt
      841 ctacattgat cgcattggat gcatagttca ggatttattt cagcttttgg caaaccataa
      901 taatctgttt tctttggttc agaactttgt cctgttatga ggtgcaggtt aggtgcatct
      961 acatcacaat cagcataagc cctggcgttg gaaagcttta taaacgcact ggctattgtt
     1021 gtttttccag taccgccttt tccactaagg attagtagtt gtttcatgct gcacctcctt
     1081 tttcactgtt tctagtagag aagaaaataa tgcattgtat tttttgcttt ctctggctgc
     1141 aatttctgcg tttgaattca acattccaag ttcattatca aagggaattc ggcccaatat
     1201 cttgatgttt ctttccaagc agaacttttc tgcaggattt tctccttcca gacatttgtt
     1261 aagaaccaca cctaatggct tgttaaataa cttgactagt tcgtatacca tattgaggtt
     1321 atgaacacca aacaatgtag gttcagccac caatatacaa taatctgcat ctttaatact
     1381 ttccattaca atacaggcac ttccgggagg gcagtcaata aaggtcagtt tgtttgagtc
     1441 ccgtttattc tcagctagaa gcttttttat gatagggata cccgaagctt cacccgtgtt
     1501 caatattcct gtgtatacca ttacctgatc ggaaattcct ttttgaactt ttccaataac
     1561 cttttctttc tctattagtg ccttttcagg acaaagaatg acacaaccgc cacaggagtg
     1621 acaaacctcc ccaaaaataa tcagtttctc ttttatatag gcaagagcat taaatttgca
     1681 aaaatcaaca cactttcgac atccattgca aagcttatcg tctacccttg gaattctaac
     1741 cgatatttct tcctcttgaa catcgtcagg tttaaagaat agatgtccat ttggttcttc
     1801 aacatcacag tctatatatg atgatgtctt cgcttctgca gctaagttca ccgataccag
     1861 tgtttttcca gtgccaccct tgccgcttag cacagctatc ctcatatcaa tttcctccgt
     1921 gcccatgaaa tccggcatga atttcatcca gcaaagaaag tcttccggct atatagtcat
     1981 caatattttc ctttactgaa gtgttggtcg ttttatatat tttgatatca gcagctccta
     2041 acacatcagc ggcattttgt ccacatcggg gtgttaacaa ggcatttacc ttgttatcaa
     2101 ctattgtttg tgcagcttta atccctgcac cgcctgtgct tgctgctgca ctgttgtcga
     2161 gaaatgtact ttccttagta tctatatcat agatgaggaa atatggagta cgtccaaagg
     2221 atacgcatac attcgacttc aagcttttct catctaccgg aattgctatt ttcataagaa
     2281 tcctccattc ttttaactta taaataatta ttgattgcat tacgcaatgt gcttactcct
     2341 aagtttgaga aatatatatg atcaatctcc tcgatcccct ttaccagtaa agcccactcc
     2401 g
//
LOCUS       DAOSDD010000418         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15488_length_2389_cov_3.407027, whole genome shotgun sequence.
ACCESSION   DAOSDD010000418 DAOSDD010000000
VERSION     DAOSDD010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15488_length_2389_cov_3.407027"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1376
                     /locus_tag="PK733_19855"
     CDS             72..1376
                     /locus_tag="PK733_19855"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOJ12826.1"
                     /translation="MDNSSIVDNISVALPEGAEYVIWTLNKNNYEAFLVGGCVRDILL
                     GIEPKDWDIATNALPEDIKIIFKNTADTGLRHGTITVMAHNQAIEVTTFRVEGKYSDH
                     RRPDKVIFTSYLEQDLGRRDFTINSIAYHPQKGIVDPFKGIDDLKKGIIRAVGEAGKR
                     FEEDALRMLRALRFSAQLGFNIDTVTMRSIKNKKALIGSISKERIRNELESILLSRNP
                     SKFILLKETGLLALILPELDKNSFNKNKYILDGVESVKRDRILRWAMLLHEESEDSAC
                     SVLKRLKFDNYTIKEVYRLIKYHNININPVPECVKKVVSIIGDELFLKLMMVRKAILN
                     AEIINCSYMSNMLKYSDNDKYYEKEMGKADIIMHIYNSAKEKRECMNIKDLAVNGSDL
                     INIGFEQGKKIGEVLELLLDTVMLIPGMNKREELLKLAAQYL"
     gene            1749..>2389
                     /locus_tag="PK733_19860"
     CDS             1749..>2389
                     /locus_tag="PK733_19860"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HOJ12827.1"
                     /translation="MKGIFSALLGVFDENYQPDIDGINAVVRHNIEHCKVDGLYVCGS
                     TGENFLINTQAKKIILKTAAEAAKGESKLIAHVGSNIIEETLELSRLAAELSYDAISA
                     VTPYYYKFTSKEIRDYYRYIADNSSLPLIIYNIPMLTGVSLNRNDLSELLSYKNIIGI
                     KYTANDLYMLERLRVECKDAYLYSGFDEMLLSAAVLETDGAIGSTYNIIGHWA"
BASE COUNT          867 a          327 c          483 g          712 t
ORIGIN      
        1 tatgttgcat aaaaaagtcc aaaaatgata tcattataaa aaacataaga taagcagaag
       61 gtgttaaatt aatggacaac agcagtattg ttgataatat ttcagttgca ttgcctgaag
      121 gggcagaata tgttatctgg acattaaata agaacaacta tgaagccttc cttgtaggtg
      181 ggtgcgtaag agatatcctg cttggtattg agccgaaaga ttgggatatt gcaacaaatg
      241 ctttacctga agatataaag attattttca aaaacactgc tgatacaggc ctaagacatg
      301 gtacgataac tgttatggct cataaccagg ctatagaagt tacaacattt agagttgagg
      361 ggaaatattc tgaccatcgc aggcctgata aagtgatttt tacatcctat cttgagcagg
      421 atcttgggag aagggatttt acaataaatt ccattgcata tcaccctcaa aagggtattg
      481 ttgacccttt taaaggaatt gatgacctta aaaaaggtat tatccgtgca gttggtgaag
      541 caggcaaaag atttgaagaa gatgctttga ggatgttaag ggctttaagg ttttcggctc
      601 aactgggttt caatattgat acagtgacta tgagatcgat aaaaaataaa aaagctttga
      661 ttggtagtat cagtaaagaa aggataagga atgagctgga aagcatcctt ttatcgagaa
      721 atccatctaa attcattctt ttaaaagaaa ctggtctttt agcgcttatt ctgcctgaac
      781 tggacaagaa ttcttttaac aaaaataagt acattttgga tggtgttgaa agtgtaaaaa
      841 gagaccgtat attaagatgg gcaatgcttc tacatgaaga aagtgaagat tctgcatgtt
      901 ctgttttaaa aagattgaaa tttgacaact atacaattaa ggaagtatac aggcttataa
      961 aatatcataa tataaatatc aatccggtac ctgaatgtgt aaaaaaagtg gtaagtatta
     1021 tcggagacga attgtttctt aagcttatga tggttaggaa ggcaatttta aatgccgaaa
     1081 tcattaattg ttcatatatg tcaaatatgt taaagtactc tgataacgat aaatactatg
     1141 agaaggaaat gggtaaagct gatataatta tgcacatcta caacagcgct aaagaaaaaa
     1201 gagaatgtat gaatattaaa gacttggcag ttaacggaag cgacttaatt aatattggat
     1261 ttgaacaagg gaagaagata ggtgaagtac tggaattgct cctggatact gttatgctaa
     1321 tacccggtat gaataaaagg gaggagcttt tgaagcttgc agcccaatat ctttaagacg
     1381 tgaattacaa atttttcctt gccttaaaca taacaacccg taagctttgt ttaaatcata
     1441 tactcaaaaa agcaaaaatt tacaaaagaa tataaagaat taaaataaaa acattttcaa
     1501 aaaaatatgt tgacatttgt cgtcataagt agtacaatct acatacaaca ttagacagaa
     1561 aacttctaaa aaatataaag attatttatt ttgttgcata agaggctttt aagaattatc
     1621 aagaataaat aggagtgtga gtgttaatag caaagcttta atgttggtat atgcataaat
     1681 atatacgttg gtagtgtcaa ataagtaatg aaaactgatg ataaacgtat atgatgggag
     1741 atagtataat gaaaggtatt ttttctgcac ttctgggtgt atttgatgaa aattatcaac
     1801 cggatattga cgggatcaat gcagtggtaa gacacaatat tgaacactgt aaggttgatg
     1861 gtttatatgt atgtgggagc accggtgaga attttctcat aaacactcaa gccaaaaaga
     1921 taattttgaa aaccgcagcg gaagctgcaa aaggtgaaag taaattaatt gcgcatgtag
     1981 gaagcaatat tattgaagag acattggaac tttcccggtt agccgcagag ctcagttatg
     2041 atgcaatatc cgcagttacc ccgtactact ataaatttac ctctaaagaa ataagggatt
     2101 actacaggta tattgcagat aatagtagtc tgccgttgat tatttataat attcccatgc
     2161 taactggcgt cagcctcaat aggaatgatt tatcagagtt gttgtcatat aaaaacatca
     2221 ttggtatcaa gtatactgct aatgatttat atatgcttga aagacttcgt gtagagtgca
     2281 aagacgccta tctgtattcc ggtttcgatg aaatgcttct atcagcagca gttctagaga
     2341 cagatggtgc tattggcagc acctacaata tcatcggtca ttgggcgaa
//
LOCUS       DAOSDD010000419         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15674_length_2366_cov_3.779749, whole genome shotgun sequence.
ACCESSION   DAOSDD010000419 DAOSDD010000000
VERSION     DAOSDD010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15674_length_2366_cov_3.779749"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PK733_19865"
     CDS             complement(<1..767)
                     /locus_tag="PK733_19865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ12828.1"
                     /translation="MDTVGNIDIAGNIQKNNSIKNELNSCDCKKDINKLVEKLSLFNK
                     MYNLVRVVDPVKKRVYECKEDNFCELDSYCYQFWKQDKICDNCISMRAYRDNDTFIKI
                     EYVKEKVFIVTAVPVNLGDKIVIVELLKDVTSSMVVGDDVVQGSNEIYSIINNLNQIA
                     IKDSLTELFNRRYINERLPVDILNSVIMNKPISIIMADLDHFKSVNDKYGHVNGDMVL
                     KAFAETLTGSVRKGKDWVARYGGEEFLICLPETDTNTA"
     gene            complement(893..2005)
                     /locus_tag="PK733_19870"
     CDS             complement(893..2005)
                     /locus_tag="PK733_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12829.1"
                     /translation="MKKYLPVNNSTSIKIISPIDGDMLNERDGKTVGGSLFTKVKVTA
                     PNGSKISINGISALYEDGLYTAEVQLKCYSNVIKVVEENSGCSESMIVYWLKNYAGKY
                     RLSLDDNIWFLQDIAENSHNYKSIFDNPYLGFFKQVHDTYGTKVHINIYYHTDDGFNI
                     TQMPAKYKSEWQDNSQWLRLSFHALANKPDKPYINATYDEMKKDCDLVLNEIRRFAGG
                     GLAGPVTTLHWGEATVEGCRALRDSGYKGLVADFNVDNELAERIPVSYYLDIEKRRNI
                     FRRFIWKDNKEDIIFIRSAIITDCHKVDKIGPFLDNIYKDPHQSAYMDLLIHEQYFYP
                     HYTAYQPEYRDKVLTVVKWAVEKGYKPAFLDECIFE"
     gene            complement(2166..>2366)
                     /locus_tag="PK733_19875"
     CDS             complement(2166..>2366)
                     /locus_tag="PK733_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12830.1"
                     /translation="GLITNDQGDKHIERINEMQNNISENGFPNGMRLPDGTKSPGRMK
                     FHKGMEMKKGPFDGTVKQGKIQ"
BASE COUNT          695 a          497 c          292 g          882 t
ORIGIN      
        1 gcagtgttgg tatctgtttc aggaagacaa atcaaaaact cttctccacc ataccttgcc
       61 acccagtcct taccttttct tacagaacca gttaatgttt cagcaaaagc ttttaatacc
      121 atatctccat ttacatgtcc atatttatca ttgacgcttt taaaatggtc aaggtctgcc
      181 attattatag atataggttt attcataatc acactgttca aaatatctac agggagcctc
      241 tcattaatat atctcctgtt aaagagctca gtaaggctgt ctttaatagc aatttggttc
      301 aaattattta ttatactgta aatctcatta ctgccctgta caacatcatc acctacaacc
      361 atactgcttg taacatcctt tagaagttca acaataacaa ttttatcccc taaattaacc
      421 ggcacagctg ttacaataaa gactttttct ttaacatatt caatttttat aaaggtatca
      481 ttatctctat atgccctcat agatatacaa ttatcacaga ttttatcctg cttccagaat
      541 tgatagcagt atgaatccaa ttcgcaaaaa ttatcctctt tgcattcata aactcttttc
      601 tttacaggat ccactactct cactaaattg tacattttat taaaaagact aagtttttct
      661 actaacttat taatgtcttt tttacaatcg caagaattta gttcattctt tattgagtta
      721 ttcttttgta tattaccagc tatgtcaata ttaccaaccg tatccaattt tattgcaacc
      781 tttcaccata ttaatttagt atttgctatt gcatataata tcctcattta tgaagattat
      841 gaggatatta tatcattcat atttttacaa gcaacataat tttcaatttt tattattcaa
      901 atatgcactc atccaaaaat gcaggcttat accctttctc cactgcccat tttacaacag
      961 tcagcacttt atccctgtac tcaggttgat atgccgtata gtgaggataa aaatactgtt
     1021 catgtatgag tagatccata taagcagact gatgcggatc cttatatata ttatcaagga
     1081 atggtcctat tttatcaacc ttatgacagt ctgttattat agcgcttctg ataaatatta
     1141 tatcttcctt attatctttc catataaaac gtctgaagat atttctcctt ttttcaatat
     1201 ccagataata tgaaaccggt attctttctg caagttcatt atctacgtta aaatcagcta
     1261 ccagaccttt gtagcctgaa tctctcaaag ctctgcatcc ttcaacagtt gcttctcccc
     1321 aatgtaaagt agttacaggt cctgcaagtc ccccccctgc aaatcgtctg atctcattta
     1381 aaacaagatc acagtccttt ttcatttcat cataagtggc atttatatat ggtttgtccg
     1441 gcttattggc caaagcatga aaactcaacc tcagccattg ggagttatcc tgccattcgc
     1501 ttttatactt tgcaggcatc tgagtaatat tgaaaccatc gtcagtatgg taataaatat
     1561 ttatatggac cttcgtgcca tatgtatcgt gtacctgctt aaaaaatccc aggtacgggt
     1621 tatcaaaaat agacttgtaa ttatgtgaat tttcagcgat atcctggaga aaccatatat
     1681 tatcatccag agataatcta tatttacccg cataattttt aagccagtaa actatcatac
     1741 tttcactaca gccgctgttt tcttcaacaa cctttataac attgctatag cacttaagct
     1801 gtacctctgc cgtatataat ccatcttcgt ataatgcgct tataccattt atacttattt
     1861 tagagccatt aggtgccgtt acttttacct tagtaaacaa gctccctcca actgtctttc
     1921 catcacgttc attaagcata tccccatcta tagggctgat tattttaata ctggtactgt
     1981 tattcactgg taaatatttc ttcactttta aaatatgcac cccttcatca ttatatattt
     2041 gtcaacttgt acttccttta ttataatgaa ttattttgaa tatgtccaag cttctttctt
     2101 cagaaagaaa ggataggaac ttatcctatc cttatttctt taatttttca ttgtacttta
     2161 atttttcatt gtattttgcc ttgctttacg gtaccatcaa aagggccttt tttcatttcc
     2221 atacctttgt gaaatttcat tctgccaggg gacttggttc catcaggtaa tctcatccca
     2281 tttggaaatc cattttcgct aatgttattt tgcatttcat taattcgctc aatgtgctta
     2341 tcaccttgat catttgttat caaacc
//
LOCUS       DAOSDD010000420         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15797_length_2348_cov_3.658090, whole genome shotgun sequence.
ACCESSION   DAOSDD010000420 DAOSDD010000000
VERSION     DAOSDD010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15797_length_2348_cov_3.658090"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PK733_19880"
     CDS             complement(<1..148)
                     /locus_tag="PK733_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ12831.1"
                     /translation="MNKTELINSIALKSGLSKKNSEAALNAFISSVEESLQIGEKVVL
                     VGFGT"
     gene            complement(344..727)
                     /locus_tag="PK733_19885"
     CDS             complement(344..727)
                     /locus_tag="PK733_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HOJ12832.1"
                     /translation="MNPMKNILVCVTQQKTCERLIIKAVALRNEYKGNIYVIHVAKDG
                     LHFLDNPKEGEALQYLFEIAKSIGADLSVLKSNNVIKCIADFAHEKEINCIVLGESPH
                     DQADNVFYEKLKGMLGDDINIMIVP"
     gene            902..1744
                     /locus_tag="PK733_19890"
     CDS             902..1744
                     /locus_tag="PK733_19890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOJ12833.1"
                     /translation="MKVIDASTIIDTVESLCITANYYLNNDILDVLQKKLNIEESGVG
                     AHVIEKIIENAHIARSEEIAVCQDTGMTVVFVEIGQDVHISGENIFEAINEGVRRGYE
                     KGFLRKSVVGDPIERVNTGDNTPAVVHYDIVKGDKLKITVAPKGFGSENMSTLKMLKP
                     ADGIEGVRKFVIEAVSNAGPNPCPPIVLGVGIGGTMEKAAILAKKALIRPINIRSSIE
                     YVKNLEIELLDKINNLGIGPLGLGGRTTALAVNIEVFPTHIAGLPVAVNMSCHATRHA
                     EAVL"
     gene            1755..2306
                     /locus_tag="PK733_19895"
     CDS             1755..2306
                     /locus_tag="PK733_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOJ12834.1"
                     /translation="MRYTLNTPLDKDIIKKLKAGDEVKINGIVYTARDAAHKRMVTML
                     QHGEELPFDIEGQVIYYVGPCPAKPGKIIGSAGPTTSGRMDAYTPVLLEQGLVGMIGK
                     GLRSPEVIESMMKHGSVYFGAIGGAGALISKHIVYQEVIAFPELGPEALRKLEVKDFP
                     VFVVIDSYGNNLYKIGQEKYKII"
BASE COUNT          807 a          331 c          457 g          753 t
ORIGIN      
        1 aagtaccgaa acctaccagt acaacctttt cgccgatttg aagagattct tcgacagaag
       61 aaataaatgc attcaaagca gcttcactat ttttcttgct taagcctgat tttagtgcga
      121 tagaattgat taattcagtt ttgttcatat atacaacctc ctatatttgg aattcgacat
      181 tattttataa tgaaacatgc attaagtcaa ggttttcgtc aaaaaatatg ttaattttca
      241 tttataaagt tagaattatg gacattttta ttatattatg aaaataaaat gacaaaatag
      301 caaatcatat tacaaaaaat gtaatatgat ttataacttt gaattaaggt acaatcataa
      361 tattaatatc atctcccaac atacctttca gtttttcata aaaaacattg tctgcctggt
      421 cgtgtggaga ttctcccaat acaatacagt taatctcttt ttcatgagca aaatcagcaa
      481 tacatttaat tacattatta gattttaata ctgataaatc agcgcctata gatttggcaa
      541 tttcaaaaag atattgaagg gcttcacctt ctttgggatt atctaaaaaa tgcaagccat
      601 cttttgcaac atggattaca taaatgttac ctttatattc gtttctcaaa gctactgcct
      661 taattataag tctttcacaa gttttttgtt gagttacgca aaccaatata tttttcattg
      721 ggttcaagga aatccccctt tcaccttaaa acaatttatc ccaacgatat tataccagaa
      781 taaagcagaa aaatgttgca ggatatgttt catttatatt tcatttaatt gattttttat
      841 tgcatatata ttaaaatctt atttgtatac atattttaat aaattggggg ttggcaaatt
      901 aatgaaagtt atagatgcat ctacaattat tgatactgta gaaagcttat gtataactgc
      961 taattattat cttaacaatg atatattaga tgttttgcag aaaaaactta atattgaaga
     1021 atccggtgtt ggagcacatg ttattgaaaa aattattgaa aatgcccata tagccagatc
     1081 tgaagaaata gctgtatgtc aggatacggg aatgacagta gtatttgttg aaattggcca
     1141 ggatgtccat atctcgggcg agaatatatt tgaagctata aatgaaggtg taaggagagg
     1201 atatgagaaa ggttttttga gaaaatctgt tgttggagat cctatagaaa gagtcaatac
     1261 aggagataat actcctgctg ttgttcatta tgatatagta aaaggggaca agctaaaaat
     1321 tactgtagct ccaaagggat ttggaagtga gaatatgagc acattaaaaa tgttaaaacc
     1381 tgcagatgga atcgaaggag tcaggaaatt tgtaattgaa gcagttagta atgcaggacc
     1441 taatccatgt cctcccatag tattgggtgt tggtattgga ggtactatgg agaaagcagc
     1501 aatcctggca aagaaagctt taatcaggcc aattaatata agaagcagta tagagtatgt
     1561 taagaatctt gaaatagagc ttcttgataa gattaataac ctggggatag gaccactggg
     1621 attgggagga aggactaccg ctcttgctgt taatattgaa gtttttccta ctcatatagc
     1681 tggccttcct gtagcagtaa atatgagctg tcatgctaca aggcatgcag aagcggtttt
     1741 ataaggaggg aactatgaga tatacattaa atacgccttt ggataaagat ataataaaga
     1801 agcttaaggc aggagatgaa gtaaaaataa acggcatagt gtatacagct agagatgcgg
     1861 ctcataagcg tatggtaaca atgttgcagc atggtgagga attgcctttt gatatcgaag
     1921 gacaggtaat ttactatgtt ggtccttgtc ctgcgaagcc tggtaaaata attggttctg
     1981 ccgggcctac tacaagtggc aggatggatg cgtatactcc ggttctactt gaacagggac
     2041 ttgtcggaat gatcggaaaa ggattaagaa gccctgaagt tattgaatct atgatgaagc
     2101 atggatcagt atattttggt gctattggag gcgccggtgc actaatttca aaacacatag
     2161 tttatcagga ggtaatagct tttccggagc tggggcctga agcattaagg aaacttgagg
     2221 ttaaagattt tcctgttttt gtggttatag acagctatgg taataattta tataaaattg
     2281 gacaggaaaa gtacaaaatc atataacatt agaatataac attaaatata ctgaagtttt
     2341 gcattttt
//
LOCUS       DAOSDD010000421         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15837_length_2344_cov_4.622106, whole genome shotgun sequence.
ACCESSION   DAOSDD010000421 DAOSDD010000000
VERSION     DAOSDD010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15837_length_2344_cov_4.622106"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1321
                     /locus_tag="PK733_19900"
     CDS             254..1321
                     /locus_tag="PK733_19900"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12835.1"
                     /translation="MNQLQIFEKTITHEQHEQYLFYANFTPDLEKRIRSKLQIDESIE
                     LRKHFGMYAPVDVNLKPPKNLKAKDFSQYFDDIEIPENAFINGLGVLEVPGSSYHFTS
                     YISPLRNANTLKEVENFQYPNVDGYTGDYMKETVDKAHKDNYVTVCSIVHMYEESWQI
                     RGYEQFLMDMIENPEICEYILDKIADRNLKKALAGAKAGVDMLICGDDVANQRDLMFS
                     PELWRKFMKPRWAKVYQAAREIKPDIQIWYHSDGNIESIIPELIEIGVTILNPVQPEC
                     MDPITIKEQYGKQIVLDGTIGTQTTMPFGSADEVRQVIKDRKEKLGYDGALILSPTHI
                     LEPEVPIDNVFAFIEMCGGKI"
     gene            1595..>2344
                     /locus_tag="PK733_19905"
     CDS             1595..>2344
                     /locus_tag="PK733_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ12836.1"
                     /translation="MKRLPYGISNYKMLVEENLFYVDKTMYIEKLESLHSYYIFFLRP
                     RRFGKSLFVSLLQHYYDINEKDNFETLFKDTYIGQNPTKERNSYYVLNFDFTGVTTDT
                     KEQLEESFINITKNAFELFQNRYNIHFDYLHTGSAANIFEDFFTKITTRIEKPIYVIV
                     DEYDHFANELLSFQFDFFRESISNTGFVRKWYEVLKKQTKTIVKRIFATGVSPITLDS
                     LTSGFNIADNITREEAFNEMMGFTEDEVKQMV"
BASE COUNT          883 a          298 c          429 g          734 t
ORIGIN      
        1 aagctgaaaa agcgggaaag tttaccatac aaaacaaatt tgaaacggat gttatctata
       61 tagataaaaa tgggttttca gaagtggtga attgtaatag aggaaatcta tttagtatca
      121 aattggacaa aaatgataaa tgcaaaataa cacccaattt gaattctcat taataatgag
      181 caatatgaaa tagcagcata tatatggtaa aattattatc gaattgtatt ttaaaggagg
      241 gtatagttta attatgaatc aacttcaaat atttgaaaaa acaataaccc atgaacagca
      301 cgaacaatat cttttctatg ctaatttcac tcctgattta gaaaagagaa taaggtcaaa
      361 gcttcaaatt gatgaaagca ttgaacttag aaagcacttt gggatgtatg ctcctgttga
      421 tgtaaattta aaaccaccta aaaacttaaa agccaaggac tttagccagt attttgatga
      481 cattgaaata cctgaaaatg cttttattaa tggtcttggg gttttggaag ttccgggaag
      541 cagttaccat tttacaagct acataagtcc tcttcgcaat gcaaatacct tgaaggaagt
      601 agagaatttc caatatccaa atgtagatgg atatactggt gactatatga aagaaactgt
      661 agataaggca cataaagata attatgtaac tgtttgttcc attgttcaca tgtatgagga
      721 atcatggcaa ataagaggct atgagcaatt ccttatggat atgatagaaa atcctgaaat
      781 atgtgaatac atattagata aaattgctga cagaaatttg aagaaagctt tagccggtgc
      841 aaaagccggt gttgacatgt taatatgtgg tgatgatgta gcaaatcaga gagatttaat
      901 gttttcacca gaactatggc gtaaatttat gaagccaaga tgggcaaaag tttaccaagc
      961 tgcaagagaa atcaaacctg atattcagat atggtaccat agtgatggta atatagaaag
     1021 tataattcct gaattaatcg agattggtgt aactatccta aatcctgtcc agcctgaatg
     1081 tatggatcct attacaataa aagaacaata cggtaaacaa attgttcttg atggtactat
     1141 cggtacacaa actacaatgc cttttggcag tgctgatgaa gttagacagg ttataaaaga
     1201 tagaaaagaa aaattaggtt atgatggagc tttaatatta tctcctactc atattttaga
     1261 acctgaagta cctattgata atgtttttgc attcatagag atgtgtggtg ggaaaattta
     1321 atgaaattca ctataaatgt taatgaacat gaatgtaaat agatcttaca tcagaggagc
     1381 atattttaaa agttaagaaa tatttaccag ggctattgct taccaatatt ttcttcactt
     1441 aaattatatg cacccagaat aaagatataa attgtactaa tcagggtaat aggatataat
     1501 aatattatta ttgtgtcctt attattatgt gtaaatagac aaattaaatc tataatcgaa
     1561 tgaattttga aaagtttgaa aggggagagt taagttgaaa aggcttccat atggtataag
     1621 caactataag atgctagtag aagagaattt attttatgtc gataagacta tgtatattga
     1681 aaaattagaa agcctgcatt catactatat attcttccta cgtccgagaa gatttggtaa
     1741 aagcttattt gtgtcgctgc ttcagcatta ttatgatata aatgaaaagg ataattttga
     1801 gactttattt aaagatacat atatagggca aaatccaaca aaagagagaa atagctatta
     1861 tgtccttaat tttgatttta caggggttac tactgacaca aaagaacagt tagaagagag
     1921 ctttatcaat ataacaaaga atgcttttga attattccaa aacaggtata acattcattt
     1981 tgattatttg catacagggt ctgcagctaa tatttttgaa gattttttca caaaaattac
     2041 tacaagaatt gaaaaaccaa tttatgttat tgtagatgag tatgatcatt tcgctaatga
     2101 actattaagt tttcagtttg actttttcag agaatcaata agcaataccg gatttgttag
     2161 aaagtggtat gaagttctaa aaaagcagac aaagacaatt gtaaagcgta tatttgcaac
     2221 aggagtaagc ccgattaccc tggatagtct taccagtgga tttaatattg ctgataatat
     2281 aactcgtgaa gaagccttta atgagatgat gggctttact gaagatgaag taaagcagat
     2341 ggtt
//
LOCUS       DAOSDD010000422         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15838_length_2344_cov_3.677588, whole genome shotgun sequence.
ACCESSION   DAOSDD010000422 DAOSDD010000000
VERSION     DAOSDD010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15838_length_2344_cov_3.677588"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK733_19910"
     CDS             <1..532
                     /locus_tag="PK733_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOJ12837.1"
                     /translation="LIDNHFIQYLEMNEKGVTFSRIDSDISESLKDSEKSDTENIKQA
                     QEALESLFKEALGDKKLKVQVESLKASSVPSMILLSEQSRRMQDLSRYFGGQDMSHMF
                     PQEETLVLNNNNSLIKAILMLKDKEDRKDDALMICNHVYDLAMMSHKQLEPDKMTKFI
                     ERSNIILEKIAAKMIE"
     gene            899..1705
                     /locus_tag="PK733_19915"
     CDS             899..1705
                     /locus_tag="PK733_19915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ12838.1"
                     /translation="MSYKLMAVDIDGTLLDSKGNLTDETMKAINKGLEKGMIFTISTG
                     RPIQGVQPLIEKLGLVEDLPFITYNGAMLVMGKSRDILFECSMNADDARTVINLGREF
                     GTTVMVWADNQLFADELNEKAYKYSSISGVAPILLEDADKIVAKGATKILWYDEVEII
                     NKYLNEVGKYLNENVNFYTSQPYFLEFVDKRASKAKAIEKLGEHFNIKQSEMIAIGDG
                     LNDLSMIEYAGLGVAMANANDIVKEKADYITLSNDEDGVAHVIYKFIVGI"
     gene            1912..>2344
                     /locus_tag="PK733_19920"
     CDS             1912..>2344
                     /locus_tag="PK733_19920"
                     /inference="COORDINATES: protein motif:HMM:NF040250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12839.1"
                     /translation="MSFNDNLVPQYSLSPKTAKNLSNSTISVPMMEAITPRWLLTFLP
                     WVSVEAGVYRLNKVKKCNEIRDGEDDYREKLPDLCSSHLGEHEIPETFPDYEEKPREY
                     TLSLVQTILRVNGQVMDIFNSPINQLQQQMRLTIEAMKEQQE"
BASE COUNT          838 a          327 c          496 g          683 t
ORIGIN      
        1 tcttattgat aaccatttta tacaatattt agaaatgaat gaaaaagggg taacctttag
       61 ccgtatagat tcggatatct ctgaaagctt aaaagactct gaaaaatcgg atacggaaaa
      121 cattaaacaa gcgcaggaag ctcttgagag tttatttaaa gaagctcttg gagataaaaa
      181 actaaaggta caggtagaaa gtctgaaagc ttcgtctgtt cccagcatga tactcctttc
      241 tgaacaatca agaagaatgc aggatttaag cagatacttc ggagggcagg atatgagcca
      301 tatgtttcca caggaggaga ctttggtatt aaacaataat aacagcttaa taaaggctat
      361 actaatgtta aaggataagg aagatagaaa agatgatgca ttaatgattt gtaatcatgt
      421 atacgacctg gcaatgatga gccacaagca acttgaacct gataagatga caaagtttat
      481 tgaaagaagt aatataatac tggaaaaaat agcagcaaaa atgattgagt aaatgtaagt
      541 aaataaatga gtaattatgt agttgggaat agagcgatac agtaattaat agattgaata
      601 gttgttgatg taattgttcg agtaattggc tgagaaacta agtagcgaat catgttcagt
      661 tgcaatattt acgacagtga aactttttcg aaagcataat gtattaaagc tttcgaaaac
      721 gctacccggc ctgaagtgga aatggcttct taattagtaa agctccatgg aatttcgtta
      781 tcagatataa tacctgtgat gtgaagaaaa taaggtgttt aagcgtttta ctatctggtc
      841 acgaatttat gtgtaactgt actttataaa gaaatggact taaggaaggg gtaattaaat
      901 gtcctataag cttatggcag ttgatattga tggcacatta ctggatagca aaggaaactt
      961 aacagatgaa actatgaagg caataaataa agggctggaa aaaggcatga ttttcaccat
     1021 ttctacaggc cggcctatac agggtgtcca gcccttaatt gaaaagcttg gattagttga
     1081 ggatttaccc ttcattacct ataatggtgc tatgttggtt atgggaaaat ccagagatat
     1141 actttttgag tgcagtatga atgctgatga tgcaagaaca gtaattaacc ttggaagaga
     1201 gtttgggact actgtaatgg tatgggctga caaccaattg tttgcagatg agttaaatga
     1261 aaaagcatat aagtacagca gtatatcagg agttgcacca attttattgg aagatgcgga
     1321 taagatagtt gcaaaaggtg ctacaaaaat actttggtat gatgaggtag aaataatcaa
     1381 taaatacctt aatgaggtag gaaagtatct gaacgaaaac gttaatttct atacatcgca
     1441 accatatttt cttgagtttg ttgataaaag agcatcaaag gcaaaggcta tagagaaact
     1501 gggagaacac tttaatataa agcaaagtga aatgatagcc ataggagatg ggctaaatga
     1561 cctttccatg atagagtatg ccggccttgg tgttgctatg gcaaatgcaa atgatattgt
     1621 aaaggaaaaa gctgattata taactttatc aaatgatgaa gatggagtag cacatgtaat
     1681 ttataagttc atagtgggga tatagaggtt cctaaaatta catattcaag gaaccgtatt
     1741 attgctacta tatctatatc tatatctata tctatgttat ttttgttaat tggttactta
     1801 tacttgttat gctaatcact tctgatggtt cggtagcata tattatacag atataatatt
     1861 tttttgttaa gtaaaacaac aattttgaag tatacatgag aggtgttatg tatgagtttt
     1921 aatgacaatc tggtacccca atacagttta agccctaaaa ctgcaaagaa tttgtccaac
     1981 agcacaatat ctgtccctat gatggaagct attaccccac gatggcttct tacttttctt
     2041 ccatgggttt ctgtggaagc gggcgtttac cgtttgaaca aggtaaaaaa atgtaacgaa
     2101 ataagggatg gagaggacga ttaccgtgaa aaactgccag atttatgttc gtcccattta
     2161 ggagaacatg aaattccaga aacctttcca gattatgagg aaaaaccgag agagtatacc
     2221 ttgagtttag tacagacaat tttgagggta aatggtcagg ttatggatat tttcaactca
     2281 cccatcaacc agctccagca gcagatgaga cttaccatag aagcaatgaa agagcagcag
     2341 gaat
//
LOCUS       DAOSDD010000423         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15857_length_2342_cov_4.980761, whole genome shotgun sequence.
ACCESSION   DAOSDD010000423 DAOSDD010000000
VERSION     DAOSDD010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15857_length_2342_cov_4.980761"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK733_19925"
     CDS             <1..453
                     /locus_tag="PK733_19925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ12840.1"
                     /translation="VFAYANHKVLKSWPVDDCIVSIMKFPNGVIGKVFVSIGCKRKYT
                     MRSVFYGDKGTIIVDTLLPYITMFKDTLYDPVYRYTSQSTETIPVQLDVSVATHNISA
                     EMDEFVDIILNDKSVITDGIQGASTVSACIAAVESTHTGETVFPVYDF"
     gene            543..1706
                     /locus_tag="PK733_19930"
     CDS             543..1706
                     /locus_tag="PK733_19930"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12841.1"
                     /translation="MNIKNGTKSGMTSKERVLAAFKREEVDYTPCAPFINNLTSVQRC
                     GLPYTFPFGSSQLEAITYMQETFQTDMIVQFHLSNQIDESVSSKIWYENDILHKSWDT
                     PSGTIHSSIRYTEKWPYGYDIGLGHDFEAHNVEPWLKTMQDVECIKHLIQPLTKKGDL
                     DVLKFSFMECKNLADKYNLPIMAQVGAGITRALQLFGPAEMCIKAIEEPEIIERFLEI
                     EHEATMHKIEFAADLGIDIIRRNGFYESCDFLSPKMLEHFLKDYLISEARLAHQISKV
                     CVYTLNTGIMPMLDFIAELEFDSIINVDMAFKNMDLYALRNRVCDKKGILIGPSSPYH
                     MCSENPEVVRDAVRKIFSVFGKRGLLLGSCASMQPYMPWENMVVMIDEWKKLR"
BASE COUNT          807 a          336 c          437 g          762 t
ORIGIN      
        1 gtgtttgcat atgctaacca taaagtcctt aaatcatggc cagtagatga ttgtatcgta
       61 tctataatga agttccctaa tggtgttata ggtaaggtct ttgtttctat tggatgtaaa
      121 agaaagtata caatgcgttc ggtcttctat ggtgataaag gaactatcat tgtagatact
      181 ttattaccat atattacaat gtttaaagac actctttatg atcctgttta taggtatact
      241 tcacaatcaa cagagacaat accggttcag cttgatgtat ctgtagcaac gcataatata
      301 tcagctgaaa tggatgagtt tgtggatatt atattaaatg ataaaagtgt cataactgat
      361 ggaatacaag gtgcctcaac ggtatcggct tgtattgctg cagttgagtc gactcataca
      421 ggagagacag tatttcctgt ttatgatttt tagatatata ttatatagca aagctttatt
      481 gttttatttg ttaaattttg ctattcatta attaaaacac tggaaaagga tgataatatt
      541 atatgaatat taagaatggt actaaatccg gaatgacttc aaaagaaaga gtattagctg
      601 cattcaaaag ggaagaagta gattacactc catgtgcacc ctttataaat aatctaactt
      661 ctgtccaaag gtgcggtctt ccttacactt ttccttttgg atcttctcag ttggaagcta
      721 taacatatat gcaggaaaca tttcaaacag atatgatagt tcagtttcat ctcagtaatc
      781 agattgatga atcagtttca tcaaaaatat ggtatgaaaa tgacatttta cataaaagct
      841 gggatactcc ttcaggaact atacactctt ctataaggta tactgagaaa tggccctatg
      901 gatatgatat aggactagga catgattttg aggcacataa tgtagaacct tggctaaaaa
      961 ctatgcagga tgttgaatgt attaaacatt taattcaacc tttaactaaa aagggtgatc
     1021 tggatgtttt gaaattttca tttatggaat gtaaaaacct tgctgataaa tataatctgc
     1081 ccataatggc tcaagttggt gcaggcataa caagagctct ccaattgttt ggtcctgcgg
     1141 aaatgtgcat taaggctata gaagaaccgg aaataataga acggttcctg gaaatagaac
     1201 atgaagcaac aatgcacaag attgaatttg ctgctgattt aggtatcgat attataagaa
     1261 gaaatggttt ttatgaatca tgtgatttct taagccccaa aatgcttgaa cactttttaa
     1321 aagattatct tataagcgaa gcacgattgg cacaccagat ttcaaaagtt tgtgtttata
     1381 ctttaaatac tggaataatg cctatgttgg attttattgc agagcttgag ttcgatagta
     1441 ttataaatgt ggatatggca tttaaaaata tggatcttta tgcattaagg aatcgtgtat
     1501 gtgacaaaaa aggaattctt atcggaccca gcagccctta tcacatgtgc agtgaaaatc
     1561 ctgaggtagt aagagatgct gtaagaaaga tattcagcgt atttgggaaa agagggctgc
     1621 ttttaggcag ctgtgcatca atgcagccgt atatgccatg ggaaaacatg gtagtgatga
     1681 ttgatgaatg gaaaaaacta agatgatata ttattgtcct aaaacaacag acaaaaaaac
     1741 aggtttttat aaatataacc tcctaagcta agcgtatcaa tggttataag agaaattttt
     1801 actgaaattc ttttagaata ttcttaatca tgagagcaaa ttacttgtag cctgtatcca
     1861 gagtattcta aactaccgaa tactgactac tggctactga ttacttgcag ctatgctgcg
     1921 gtatgatata aataagttta ggaattcaat aacatatatt atacacaact tcgtaaagga
     1981 tcatggggct tgtggttatc aaggagaaca aatatcgtgt tttgtacgat aattgttctt
     2041 ttgccagcat tgtgtaaagg catgtcctca aggaactata aaaatggata atgatagata
     2101 taaggatttc cagtctggaa tgactttttg cataaaagag gttctaaaag tatttaaaac
     2161 tcaaaatgtt ttctatagta tatttatgtt ccgcttttta tgcaaagctt attatgttaa
     2221 ttttaaccat tatctatttt tcaatgttca aaaaagtgca aaactatagt aaattaaatg
     2281 cactcaaaca gctctgagga gtaaaaattc ttcggaataa aatgcgacaa atcctttcaa
     2341 aa
//
LOCUS       DAOSDD010000424         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_15893_length_2339_cov_3.537215, whole genome shotgun sequence.
ACCESSION   DAOSDD010000424 DAOSDD010000000
VERSION     DAOSDD010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15893_length_2339_cov_3.537215"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PK733_19935"
     CDS             <1..297
                     /locus_tag="PK733_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ12842.1"
                     /translation="ILAGKTRMRPILMTAITTIFGLSTLSIGVGTGAEMVQPLAIVSI
                     GGLTYATILTLFVVPVMYHLLNRDDTVNRDDIVNRDDTANRDNLDSNSIEIKDR"
     gene            304..876
                     /locus_tag="PK733_19940"
     CDS             304..876
                     /locus_tag="PK733_19940"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ12843.1"
                     /translation="MTENRRENLLNAATDVFSQYGFHGAKMEDIAKKAEIGKGTIYGY
                     FDSKRSLFYEMIKYRIEEYRKGLDSALNIDGSLEEKLYAQYVFHEAYLNKYIDITQII
                     MREKEVLPKELIMELAAEMDKLFSRMKRAVEEAIHRKELRKDLDPKLAAIIIIGSIGQ
                     FYGQKIYCQKKGHDNIDLMDLIDTVLKGLR"
     gene            1248..2132
                     /locus_tag="PK733_19945"
     CDS             1248..2132
                     /locus_tag="PK733_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOJ12844.1"
                     /translation="MFGYIMPDKPELKIKEYEAFNAFYCGVCKSIGRRYGNIPRLILN
                     YDSAFLAILLWSLEDKNLEVNRERCIVHPIKKRLLVIKNDIIDYSADINIILSYFNLE
                     DNKRDEGSIIAKTGTVILKPIYKKLRRKYRKKCDIIEQRLEELATLEKNMCTSMDMAA
                     EPFARIMEEVIAYNPLCKDENVEKIIRWLGYNLGKWIYLLDAFDDMEDDAKKNRYNPF
                     LHKKDLKKEEISELKNRIRSRVEFNLIYSLDQIGKAYELLHAKNVRGLIENIIYLGMY
                     KKTEKILGVGRCKEVESI"
     gene            2119..>2339
                     /locus_tag="PK733_19950"
     CDS             2119..>2339
                     /locus_tag="PK733_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ12845.1"
                     /translation="MNPYEVLGVKEGANDEEIKRAYRELVKKYHPDQYRDNPLAKLAE
                     EKLREVNEAYEYLMKQSSTRSGRTSRDYS"
BASE COUNT          892 a          305 c          492 g          650 t
ORIGIN      
        1 atattggcag ggaaaacccg tatgagacct atattgatga cggctattac tactatattc
       61 ggattatcca cattatccat aggagtggga acaggtgctg aaatggtaca gcccctcgcc
      121 atagtatcta ttggaggatt aacctatgcg acaatactta ctttatttgt agtgccggtg
      181 atgtatcatt tgctcaatag agatgatact gtcaatagag atgatattgt caatagggat
      241 gatactgcca atagggataa tctcgattca aattccatcg agataaagga taggtgaaaa
      301 taaatgacgg aaaacagaag agaaaattta ttaaatgcag caacggatgt attttcccaa
      361 tatgggtttc acggagccaa gatggaggat attgccaaga aagcagaaat aggtaaaggt
      421 actatatatg gatattttga cagtaagaga tccttgttct acgagatgat aaaatataga
      481 attgaagaat acaggaaagg cctcgacagt gccttaaata tagatggcag cttggaagaa
      541 aagctatatg ctcaatatgt atttcatgaa gcatatctaa acaaatatat agatataacc
      601 caaataataa tgagggaaaa ggaagtgctt cctaaggaat taataatgga attagcggct
      661 gaaatggata aattatttag caggatgaaa agggctgttg aagaagctat tcatagaaag
      721 gaactgagaa aagacctgga tcctaaatta gcagctataa taattatagg ttccattggc
      781 caattttatg gccagaagat ttactgccaa aaaaaaggac atgataacat agacttgatg
      841 gatttaatag atactgtgtt aaaagggcta agataaagta attattccag tcccaaataa
      901 ggaaaccggt aacacaggcg acggctcacg acatccgtgt cgtgatttac gaatttttcc
      961 ttgccttaaa cataacaacc cgtaagcttt gttcaaatca tatacttaaa aagcaaaaaa
     1021 tgtttagcgc tccttttttg ccgtgcctgc tgcgatagca gctggcaaaa ggcaaaattc
     1081 ataggagcgg ttagtaattt tttgcttttg ctaatcgaat aatgccatat tttttggtca
     1141 tatacagaaa atgcgcccat aagtttgtaa attagttgac tgttatagta tattatatat
     1201 aatggatgta aaaattagcg caggaattta cttaaaggat ggtatgattg tttggctata
     1261 ttatgccgga taaaccggaa ctaaagataa aagaatatga agcgtttaat gccttttatt
     1321 gcggagtatg caaatcaata ggaagaaggt atggtaatat tccacgtctt atattaaact
     1381 atgattctgc attccttgca atacttctat ggtcattgga agataaaaat cttgaagtta
     1441 acagagaaag atgtatagtt catccaatta aaaagaggct tcttgttata aagaacgata
     1501 taattgatta ttcagcagat attaatataa tactttcata ttttaattta gaagataaca
     1561 aaagagatga aggttcaatt atagcaaaaa ccggaacggt aattttaaaa ccaatatata
     1621 aaaaattaag gaggaaatat cgtaaaaaat gtgatataat tgaacaaaga ttggaagagt
     1681 tagcaacact ggagaaaaac atgtgtactt caatggatat ggcagcggaa ccttttgcca
     1741 ggataatgga agaagtaata gcatataatc ctctgtgtaa agatgaaaat gttgaaaaga
     1801 ttatacgttg gctaggttat aatttaggaa aatggattta tttacttgat gcctttgatg
     1861 atatggaaga cgatgcaaag aaaaacagat ataacccatt tttacacaaa aaagatttga
     1921 aaaaagaaga aataagtgaa ttaaagaata gaataagaag cagagttgag tttaacttaa
     1981 tatattcatt agatcagata ggaaaagcat atgaattgct tcatgcgaaa aatgtgagag
     2041 ggttgataga aaatattatt tacttgggca tgtataagaa aactgaaaaa attctaggag
     2101 tggggagatg taaggaagtt gaatccatat gaagtattag gagtgaaaga aggagccaat
     2161 gatgaagaaa taaaaagagc ctacagggaa ttggtgaaga aataccatcc tgaccaatac
     2221 agggataatc ccctggcgaa attggctgaa gagaaattaa gagaggttaa tgaggcatat
     2281 gagtacctga tgaagcaatc atctacgagg tccggtagaa cttcaaggga ttacagtca
//
LOCUS       DAOSDD010000425         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_17996_length_2124_cov_6.552924, whole genome shotgun sequence.
ACCESSION   DAOSDD010000425 DAOSDD010000000
VERSION     DAOSDD010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17996_length_2124_cov_6.552924"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PK733_19955"
     CDS             complement(<1..1346)
                     /locus_tag="PK733_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12846.1"
                     /translation="MKATRLLSLLLSMFLILSIFPMPVTAAGSQLEFSEISPEVSPEI
                     LSEISPETSSRVFNETASNDTTLMSLLQFDKDLPRYDWGLQAPAPAKFTGVTYTLADN
                     VVKVNPDVSKNILSMQQVENPNPTNNQSIRNIIEQGGLYKADQLKKATGLSIIKPGSV
                     FVDLENDLAFKVPTLPDETGGAYQGYVPVIRPEMQEIIKDFNIPAQKVRLNQSNVTHF
                     AKDANGNSLDSYLKKPGQTYVMSHNSEMEPLKPTHLKDVIAEFSFPEAGITLNGVTPS
                     GGMVSVNVKGYLAIGDMDVDGYYEKWKYGFWFSVAEEIQLQATVAMELHEEVRIPILG
                     VDIGFDSDIGSIAGGLFIIVGVDGKFTLKMEARQWTKLNKVGLKGKCVFYVPCTISPL
                     HEFGDSGFYLDSHFNGAIDGYVKGGALLELSLLGIDIIGAGVFAGMGVNLTVSGEY"
BASE COUNT          664 a          441 c          366 g          653 t
ORIGIN      
        1 atatattctc cggagacagt taaatttacc cccataccgg caaatactcc tgctccaatg
       61 atgtcaatcc caagcagact gagttcaagt aaagccccac ctttaacgta tccgtctatt
      121 gctccgttga aatgtgaatc aagataaaaa ccagaatccc caaactcatg gagcggtgat
      181 atggtacaag gcacgtaaaa cacacatttc cctttgagac caaccttatt aagctttgtc
      241 cactgccgag cttccatctt aagtgtaaac ttgccatcta cacctacaat tataaaaagc
      301 cctcctgcaa tgctgccaat atccgaatca aagccaatat ccacgcctag aatcggtata
      361 cgaacctctt catgcaattc cattgcaacc gtggcttgaa gctgtatttc ctctgctaca
      421 gaaaaccaga agccatactt ccacttttcg taatatccat caacatccat atcgccaata
      481 gccagataac ccttaacatt taccgatacc attcctccac ttggagttac accattgagc
      541 gtaatacccg cttccgggaa actaaattct gctataacat ctttcaaatg ggtaggcttc
      601 agaggttcca tttctgaatt atgggacatg acgtaggttt gccccggctt tttcaaataa
      661 ctgtccagac tgtttccatt tgcatctttg gcaaaatgtg ttacatttga ctgatttaac
      721 ctgaccttct gagcaggaat attgaaatcc ttgatgattt cctgcatttc aggccttata
      781 acgggtacat atccctgata tgcaccacct gtttcgtcag gtagtgtggg tactttaaac
      841 gcaaggtcat tctctaaatc tacaaatacc gagcctggct tgatgataga aagaccggtg
      901 gcttttttaa gctgatcagc cttgtacagt ccgccttgct cgataatgtt tctgatactt
      961 tggttattcg taggatttgg attttccacc tgctgcatgg agaggatgtt tttggataca
     1021 tctggattta ccttcaccac attgtccgca agagtataag tgacgcctgt gaatttagcc
     1081 ggtgccggtg cctgtaaacc ccagtcatac cttggcagat ccttatcaaa ttgtaataac
     1141 gacatcaggg tagtatcgtt tgaagcagtc tcattaaaaa ctctagaaga tgtttcagga
     1201 gatatttcag ataatatttc gggagatact tcaggagata tttcagaaaa ttctaattga
     1261 ctgcctgctg cagtcacagg catcgggaaa atggaaagaa ttaaaaacat ggataaaagt
     1321 agtgatagca gtcttgttgc tttcataggc tagtctcctc atctctttaa atttattcac
     1381 tgtcactcat aatcgcttat agtaactctt aatcgatcat agctacttat agctgcttat
     1441 tgttaatcag agccacttat attcactcat attaacactt aatcaaatgt ttaccaatat
     1501 tataatacta tgaaaataaa taaaatatat acggaattct gctcacttta acaaaattct
     1561 gatctatcct aacagatcta atagacctaa cagatctaac agaattctga cttgttataa
     1621 tgggataatg agataatgag ataatgagat aatgagataa tgagataatg tacttatctt
     1681 ttccgtattt gcttgtggta atctgatgga gaaaagcctg ttctctcttt aaatatatta
     1741 gcataatgtc catgatattg gataccgcac ctggcaaagg cttcttcaat agaaaggttt
     1801 gtattcagca acgcatcttt aaccttgttg atttttacga tagaccatgc agaacgttcc
     1861 cttttttaaa tgggagatga atgcatgact accttatttc ccaaatctat cgctgctaat
     1921 ctatattaag aaagaaaaaa ccaaagcgaa tgtcaaaaag tccagaaaaa gtttttgaaa
     1981 aagattgaag ttttctcttg cgtatacaag caaaatatgg tactatttaa ttaaagaaca
     2041 tctgtttgac aaaggaggtg aaattattgg caactataac ttttgttgtt aagttgaaga
     2101 aacctacaag agttaaacac aaac
//
LOCUS       DAOSDD010000426         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_18991_length_2029_cov_4.997467, whole genome shotgun sequence.
ACCESSION   DAOSDD010000426 DAOSDD010000000
VERSION     DAOSDD010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18991_length_2029_cov_4.997467"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK733_19960"
     CDS             <1..236
                     /locus_tag="PK733_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12847.1"
                     /translation="GYIDIALLRREPIDGRLKKKGIYMLNDNLIRFWYRCVPRNMSRI
                     QEKLGKLIYKKIIAPFLGMVLGESCIILQYSIN"
     gene            436..1203
                     /locus_tag="PK733_19965"
     CDS             436..1203
                     /locus_tag="PK733_19965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ12848.1"
                     /translation="MYNLLVVEDEDNIRKGIENNNDWQSLGLKVTGSVSNGLDAFDFL
                     SRNIVHVVLADIRMPKMDGLELMRQCKMIKLSTYFVFLSAYDSFQYVQMAINYGAKGY
                     LLKPLKKTDLLNVFQNIIDELDVQYGIGGHQEDMLSGCESITGIPYFVKKAMLYVNEN
                     YENKHTLDSMSQLLYVSPEYFGITFKKTTGMSFVDYYTSVKMNKAKELMTYLSYSIKE
                     TAIKVGYEDYSYFCKLFKKHTGFTPLEYRRRILLKQL"
     gene            1255..>2029
                     /locus_tag="PK733_19970"
     CDS             1255..>2029
                     /locus_tag="PK733_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12849.1"
                     /translation="MKHKKRIGFRLFVLLVSLVFELIVVFSVGVYLFTGVDKSFYEML
                     ATTEDNNLQQSIIGLNEFYTQAYNTATFMQKNVNFINMLTRFIGLNLEKDSSQLSIDE
                     INERYKLQEDIEGFIHKIVIQNRIRSLHYGKSENLLYKEIAIFMEKRILSAENIVTSA
                     DVNIFFNTGKEDDVISFLSYDELKGYGIHLTEDPTRQNAIGIEENPPCVIFNIMLNGN
                     KICSILIVLSNTYIEQNVYSSENIIVMNKENNILYQGKNI"
BASE COUNT          759 a          221 c          366 g          683 t
ORIGIN      
        1 atgggtatat agacattgca ctattaagga gagaaccaat agatggaaga cttaaaaaga
       61 aaggcatata tatgttaaat gacaatttga tcagattttg gtatagatgt gtacctagaa
      121 atatgagcag aatacaggaa aaacttggga aattaatata caaaaaaata attgctccct
      181 ttttagggat ggttcttggt gaatcatgca tcatattgca atattccata aattgatttg
      241 aatatttcta ttttttctat gcaatttgat gtataataaa tgtaaaataa aataattgtg
      301 actattttat tttatattca ctacatacca tatgctatat gataaaggtg taaagcaagt
      361 tcctataata aagccataca ataaggcgga ttggtaataa atttataaat tctgcattat
      421 atggggggct tgataatgta taacctatta gttgttgaag atgaagataa tataagaaaa
      481 ggtattgaaa ataataatga ttggcaaagt ttgggtttaa aggttactgg aagtgtcagc
      541 aatggactgg atgcttttga ttttctgtcc cgtaatattg ttcatgttgt tcttgcggat
      601 ataagaatgc ctaaaatgga tggattggaa ttaatgaggc aatgcaagat gataaaactt
      661 agcacctatt ttgtttttct tagtgcatat gattcctttc aatatgttca aatggcaata
      721 aattatggag caaagggtta cttacttaaa ccgctaaaaa aaacagattt attaaatgtt
      781 tttcaaaata ttatagatga acttgatgtt caatatggaa taggtggcca tcaagaagat
      841 atgttaagtg gatgtgaatc tataacggga ataccatatt tcgtaaaaaa agccatgcta
      901 tatgtaaatg aaaattatga aaataagcat actttagatt ctatgagcca gcttctttat
      961 gtatcacctg aatattttgg aattactttc aaaaaaacta caggaatgag ttttgttgat
     1021 tattatacaa gcgttaaaat gaataaagca aaggagttaa tgacttactt gtcatattct
     1081 ataaaagaaa ctgcaataaa agttggttat gaggattatt catatttctg taagttgttt
     1141 aaaaagcata caggatttac accattggaa tatagaagaa ggatcttgtt aaagcaactg
     1201 taacaatttg attgcctatt taacaaatat taaatatttt ctggagataa gtccatgaag
     1261 cataaaaaga gaattgggtt caggctattt gttttacttg tttccctagt ttttgaatta
     1321 atagtagttt tttctgtagg agtatatttg ttcacagggg tagataaaag tttttatgag
     1381 atgcttgcaa cgacggaaga taataattta cagcaatcta taataggttt aaatgagttc
     1441 tatacgcaag cttacaacac tgcaacattt atgcaaaaaa acgtgaactt tatcaatatg
     1501 ttaactagat ttattgggct taatcttgaa aaggacagtt ctcagctttc tattgatgag
     1561 attaatgaaa gatacaaatt acaggaggat attgaggggt ttatacataa gattgtaatt
     1621 caaaacagaa tacggtcttt acattatgga aaatctgaaa atcttttata taaagaaatt
     1681 gcaatattta tggaaaaacg aattctttct gcagaaaata tagttaccag tgcggatgtt
     1741 aatatttttt tcaataccgg aaaagaggat gatgtaatta gctttttatc atatgatgaa
     1801 ttgaagggat atggtataca cctaacagaa gatcctacaa ggcaaaatgc tataggtata
     1861 gaggaaaacc cgccttgtgt tatattcaat attatgctga atggaaataa aatttgtagt
     1921 atattaattg tacttagcaa tacatatatt gagcaaaatg tctatagtag tgaaaatata
     1981 atcgttatga ataaggaaaa taatatattg tatcaaggga aaaatatta
//
LOCUS       DAOSDD010000427         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_19136_length_2016_cov_5.541050, whole genome shotgun sequence.
ACCESSION   DAOSDD010000427 DAOSDD010000000
VERSION     DAOSDD010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19136_length_2016_cov_5.541050"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..1095
                     /locus_tag="PK733_19975"
     CDS             340..1095
                     /locus_tag="PK733_19975"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ12850.1"
                     /translation="MKKMIKITSVMLVIFLLASLFYGCSKNDNKPLNSNPDESTTNQE
                     TSSTQKNNSNNSDEKLFGCLKLPIVDKPITLTYFVLNGYPTVLKTFNDMKMYQIQEQD
                     TGIHIEFIHPPAGQENEQINLLIASNDLPDILELNPLGYPGGPEKLLQDNVAVEIQDM
                     LNEYAPNLKNVFERHPEAKKQVSTDSGKMYCFPWLNFHDIQLVYMGHIIREDWLKNLS
                     LEMPVTIDDWYNVLTAFKEKDPNGNGKKMKFLI"
     gene            1098..2009
                     /locus_tag="PK733_19980"
     CDS             1098..2009
                     /locus_tag="PK733_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12851.1"
                     /translation="MKYGRIDPFCYAWGLTSDFYIENGNIKYGVIEPRFKDYLSTMRK
                     WYSEGLIDPDYASTDSKQFDAKATNHIGGAFYGYAGGQVRKYMSLMEQNDPNYKLAGT
                     LYPVLEQGQKPPIGQRINMFPGNNSAIITGNNKYVKETVQWLDYNYCPKGHMIYNYGV
                     EGESYFMDNGYPRFVDKIVKTPNGFLNELTKYNRFVGAGPWENGKEAQEQYMGHPASV
                     KAIQNWSQTERGDVLPPTTPSQEDSLRLNSIMNEINTYKFEMLNRFIMGQENIDNFDE
                     FVSTIKKMQIEEAINIQQSALDRYNKR"
BASE COUNT          767 a          271 c          378 g          600 t
ORIGIN      
        1 agttatcgac tcacttgttg acatggggat ttgtagaaat agtgaatatt agaacaatga
       61 aaaaatatga aaagtggaat aaaaagcgga tcggatacta cagattacta taaaattaaa
      121 tgagcaagtt aaaagtatta gctatggtac ctatgtcaaa aaaaattgat taagagcaaa
      181 acttgataat ttatatgaca aaatatgaaa acccaagtat aaagataata attgatttta
      241 tagattcaac cacttataat tatgcttgct aacacacttg gtgacgcgtt accctggtta
      301 tgcaattttt taaaataaaa atcaaggagg attagtctga tgaaaaaaat gattaaaatt
      361 acatcagtga tgttggtaat ttttttatta gcaagcttat tttatgggtg ttcaaaaaat
      421 gataataaac cattaaatag taatcctgat gagagtacaa caaatcaaga aacatcttct
      481 acacaaaaaa acaattcaaa taatagtgat gagaagctct ttggatgttt aaaacttcca
      541 atagtggaca aaccaataac tctgacttat ttcgttttaa atggttatcc tacagtttta
      601 aaaactttta atgatatgaa aatgtatcag atacaagagc aagatactgg gattcacatt
      661 gaatttattc acccgccggc agggcaggaa aatgaacaga taaacttact aattgcttca
      721 aatgatttac ctgatatatt agaattaaat cctctaggat atccgggtgg tccagaaaaa
      781 cttttgcagg ataatgtagc tgtagaaata caagatatgc ttaacgaata tgctcctaat
      841 ttaaagaatg tatttgaacg acatcctgaa gctaaaaaac aggtttctac cgattcaggg
      901 aaaatgtatt gctttccgtg gctaaatttc catgatatcc agttagttta tatgggacac
      961 ataataagag aagactggct taaaaatctc tcattagaaa tgccggtaac tattgatgac
     1021 tggtataatg ttttaactgc ttttaaagaa aaagatccta atggaaatgg gaaaaaaatg
     1081 aaattcctta tatagctatg aagtatggca ggattgatcc tttttgctat gcatgggggc
     1141 ttacatcaga tttctatatt gagaatggta atataaaata tggagttata gagcctcgtt
     1201 ttaaagacta cctaagcact atgagaaaat ggtattctga aggtttaata gatcctgatt
     1261 atgcaagtac agatagtaaa cagtttgatg caaaagctac gaatcacata ggaggagcat
     1321 tttatggata tgcaggtgga caagtcagaa agtatatgtc actaatggag caaaatgatc
     1381 ctaactacaa attagcaggt actctttatc ctgttttaga acaagggcaa aagccaccta
     1441 taggtcaaag gataaatatg tttcccggta ataatagtgc aattataaca ggtaataata
     1501 agtatgtaaa agaaacagtg cagtggcttg attataatta ttgtcccaaa ggtcatatga
     1561 tctataatta tggagtggaa ggcgagagct attttatgga taatggatat cctagatttg
     1621 tagataagat agtcaaaact cctaatggat ttcttaatga attaacaaaa tataatcgat
     1681 ttgttggtgc aggtccttgg gagaatggga aagaagctca agagcaatat atgggccatc
     1741 ctgcttctgt taaagcaatt cagaactgga gccagacaga aagaggggat gtacttcctc
     1801 caactacacc ttcacaggaa gatagcttgc gtttaaattc tatcatgaat gaaattaaca
     1861 cttataaatt tgagatgttg aatagattca taatgggaca agaaaatatt gataattttg
     1921 acgaatttgt ttcgacaatt aagaaaatgc aaattgagga ggcaatcaat attcagcaat
     1981 cagcattaga tagatacaac aagagataga ttgaat
//
LOCUS       DAOSDD010000428         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_20013_length_1949_cov_5.842133, whole genome shotgun sequence.
ACCESSION   DAOSDD010000428 DAOSDD010000000
VERSION     DAOSDD010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20013_length_1949_cov_5.842133"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1949)
                     /locus_tag="PK733_19985"
     CDS             complement(<1..>1949)
                     /locus_tag="PK733_19985"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HOJ12852.1"
                     /translation="KILVLAEKPSVARDIAKVLNCRQNSNGYISGSKYIVTWALGHLV
                     TLADPDIYDNKYKTWNLEDLPMLPKKMELVVIKQTSKQFGIVKSLLHRDDIDELVIAT
                     DSGREGELVARWIIEKAGFRKPIRRLWISSQTDRAIEEGFAKLRPGREYDNLYMSAQS
                     RAEADWLIGLNVTRALTCKYNAQLSAGRVQTPTLAMIVDRENEIKRFVPKDYWTIQAA
                     VNGFTVQWKDKTSGQTRIFNKEQADSIASKIKEQIGQIIEVRKDSRKELPPLAYDLTE
                     LQRDANRKYGYSAKKTLSIMQRLYESHKLVTYPRTDSRYITDDIVPTLNERLKSISVG
                     PYTKLAQSLQKSKMNITKRFVDNSKVTDHHAIIPTEQYVDLSSLNSEERSIYDLIVKR
                     FIAVLSPPFEYEQTTITINVNGELLYARGKIVKSLGWKTVYDSVGQSYQGEEAYEDEN
                     DNEDEEGYYGNIQSLPDVSKGQKEKIISVKLINGKTKPPARYTEATLLSAMEHPGKFV
                     DNKILREALESASGLGTPATRADIIEKLFDTFYVERKGKELVPTSKGIQLIGLVPADL
                     KSPELTAKWEQQLSLISKGKANSSTFIGEMRDYASKLVSTVISSTEAYKHDNLTKEKC
                     PECGKYLLEVNGKKGKMLVCADREC"
BASE COUNT          500 a          430 c          332 g          687 t
ORIGIN      
        1 acactcacgg tcagcacata caagcatttt acctttcttg ccattaacct ccagcagata
       61 tttaccgcat tcgggacatt tttcctttgt aagattatca tgcttataag cttctgtact
      121 ggatattact gtggaaacca atttagaagc ataatctcgc atttcaccta tgaaggtact
      181 tgaattggct ttacctttgc taatcaatga aagctgctgt tcccattttg ctgttaattc
      241 cggtgacttt aaatcagcag gtacaagccc aattagctga atacccttgg atgttgggac
      301 taattctttt ccttttcttt cgacataaaa agtatcaaag agcttttcga ttatatctgc
      361 tcttgttgca ggagttccaa gcccactggc gctttccagg gcttctctca aaattttgtt
      421 atctacaaac ttgcctggat gttccatagc tgataataaa gtcgcctcag tatatcgtgc
      481 aggtggtttg gttttgccgt ttatcagttt tactgaaatt attttttctt tttgtccttt
      541 gcttacatca ggaagagatt gaatgtttcc ataataacct tcctcatctt cattatcatt
      601 ctcatcctca taggcttctt caccttgata tgattgcccc acactatcat atacagtttt
      661 ccaacccagc gattttacta tctttcctct ggcataaagc aattctccgt ttacattaat
      721 ggtaattgta gtctgctcat attcgaaagg agggcttaaa acagctataa atctttttac
      781 tatcaaatca taaatgctgc gttcttctga atttagagaa gataaatcca catactgctc
      841 agtaggtatg atggcatggt ggtctgtgac tttgctatta tctacgaagc gttttgttat
      901 attcattttg ctcttctgga gactttgagc taactttgta tacggtccca cagatatgct
      961 tttcaaacgt tcattaagtg taggtactat atcatcggta atataccttg aatcggttct
     1021 tggatatgtt accaatttgt gtgactcata tagacgctgc atgatagata aggtcttttt
     1081 agccgaataa ccatatttgc ggtttgcatc tcgttgaagt tctgtaaggt cataagcaag
     1141 aggcggcagc tccttcctcg aatccttccg gacttctata atttgaccta tttgctcctt
     1201 tatttttgag gcaatactat cagcctgttc cttattgaat attcttgtct gtcctgaagt
     1261 cttatccttc cattgaacag taaatccatt gactgctgcc tgaatggtcc agtaatcctt
     1321 tggcacgaat ctttttatct cattctcacg atctacaatc atagccagtg taggagtttg
     1381 aacccttcct gcagaaagct gtgcattgta tttacatgtg agtgctcttg ttacattaag
     1441 tccaataagc caatccgcct ctgctctgct ctgagcagac atataaagat tgtcatattc
     1501 cctgccgggc ctaagctttg caaagccctc ttcaatagcc ctatctgttt gtgacgaaat
     1561 ccacaaacgc ctaataggtt ttctaaagcc tgccttttcg ataatccatc ttgcaactaa
     1621 ttctccctct ctgcccgaat cagttgcaat aacaagttca tctatatcat ctctgtgaag
     1681 caggctttta acaataccaa actgttttga tgtctgtttt atgaccacaa gctccatctt
     1741 tttaggcagc atgggaaggt cttctaaatt ccaggttttg tatttattat cataaatatc
     1801 aggatctgct agtgttacaa gatgacccag cgcccaggta acaatatatt tagaccctga
     1861 aatatatcca ttgctgtttt gtctgcaatt aaggaccttt gctatatccc tcgctacaga
     1921 gggtttctct gccagaacca agatttttc
//
LOCUS       DAOSDD010000429         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_20703_length_1900_cov_4.463957, whole genome shotgun sequence.
ACCESSION   DAOSDD010000429 DAOSDD010000000
VERSION     DAOSDD010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20703_length_1900_cov_4.463957"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK733_19990"
     CDS             complement(<1..470)
                     /locus_tag="PK733_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12853.1"
                     /translation="MTALQIAKKTIKDLRTVISNLHTMYGDGYDKLQQTVGMLQKEIL
                     RLTTENEQLKKALKQDEEQEALAALVPHLKETIKDLKKENIVLKKENEKFTDQLETLR
                     NQVKKTSETSDKPSSTNIFKKPMSARVKSGRKPGGQLFSVTLFGTKTEQKSLYLV"
     gene            complement(618..1346)
                     /locus_tag="PK733_19995"
     CDS             complement(618..1346)
                     /locus_tag="PK733_19995"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOJ12854.1"
                     /translation="MLNRPKVTDDIIMQINSVITEHPDWNRTKISKHLCELWDWRMPN
                     GQVKDISCRDMLRTLDKAGKITLPKAIKGLGHASKKNKYMQTFLHDETPVETKLSALQ
                     PLRVELVEEKWQINEFKSYIAQFHYLGFDRTVGENMKYMVYSTDGRILACLLFGSAAW
                     SCHDRDAHIGWSKQSRKSNLQNMTNNHRFLIPQWIKVPHLASHILSLISRRLSADWEK
                     KYGHPIYCLETFVECDRPVQGNLV"
BASE COUNT          502 a          391 c          332 g          675 t
ORIGIN      
        1 accaaataga gtgacttttg ttctgtctta gtcccaaaaa gagtgaccga gaacaactga
       61 ccgcccggtt ttcttccgct tttgacccgg gcgctcatag gtttcttaaa tatattagta
      121 gaagatggct tatcggatgt ctcactggtt tttttcactt ggttgcgcag agtctccagt
      181 tggtctgtga acttctcatt ctcttttttt aatactatat tttctttctt aagatcttta
      241 atcgtttctt tcaaatgcgg aaccaatgct gctagagctt cctgctcttc atcttgtttc
      301 aaagcctttt ttaactgctc attttccgtg gtcaagcgca gtatttcctt ctgcaacatg
      361 ccaactgtct gttgcaactt atcatatcca tcgccataca ttgtatgaag attggatatt
      421 acagttctta gatctttgat tgttttttta gcaatctgca gagctgtcat tgggattcac
      481 cttcccgtcc aagcaactgc cgataattcc tattcaacgg atacagatag atatgtttaa
      541 tcggaagcga tgccaagtta tcagtatcat tacgccccct gcccactgtt ttcccaacat
      601 agatccagtt tgcagcctta tacgaggttc ccttgaaccg gtcggtcgca ctcaacgaat
      661 gtttcaaggc aatagatggg atgcccatac ttcttttccc aatctgctga aagcctacga
      721 gaaatgagag atagtatatg actcgcaaga tgaggcacct ttatccattg tggtataagg
      781 aatcggtgat tatttgtcat attttgcaaa ttcgattttc ggctctgttt gctccatccg
      841 atatgggcat ccctgtcatg gcatgaccag gctgctgagc cgaacaataa acacgcaaga
      901 attcttccgt cagtactata caccatatat ttcatattct ctcccaccgt acggtcgaac
      961 ccgagataat gaaattgagc aatatatgat ttaaattcgt ttatttgcca cttttcctcg
     1021 actaattcca cacgtaacgg ctgtaatgct gataatttcg tttcaactgg tgtctcatcg
     1081 tgtaagaatg tttgcatata tttgtttttt ttagaagcgt gtcctaaccc tttgatagcc
     1141 ttgggtaaag tgatctttcc tgccttatca agtgtacgta gcatatctct acaggagata
     1201 tccttgactt gaccatttgg catccgccaa tcccataatt cgcacagatg cttcgaaatc
     1261 tttgtccgat tccaatctgg atgttccgta attacactgt ttatctgcat gataatatca
     1321 tctgttacct tgggtctgtt gagcatgtga tacctccata atttttatga tttcagtgta
     1381 tcatgcttcg ttttttgagt tcagtgtttt tttaaaaagt tggaaaattt tttttcagtt
     1441 tctgttttca tataacttaa ttatggggta ccctaccact gaatagagac tatcattcat
     1501 attatccgct taattacaga gcttaagtat atttatgatt ggtggagaag ataaaaacaa
     1561 atgcattgaa acatggatat tccctgataa tatctgatat gcaaagtaat aatacttctc
     1621 tgcttgacgg aagggtggga acagataatt tcgatataag aaagccaaat gaaagtgcta
     1681 tatacatagt cctttccttc aaaggctggc atagcttttt tgtctaatga taaacaagca
     1741 taaaccctgt tttaatattt taacacaggg tttattatat attctgtttt aacaatgcat
     1801 tgtttcttat gatgacctat tcccataagc aaaatatctt tgaagccttc tccttcaaaa
     1861 accttgtagt acttcttatc tttaatttgt tttattgctt
//
LOCUS       DAOSDD010000430         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_22050_length_1808_cov_5.005134, whole genome shotgun sequence.
ACCESSION   DAOSDD010000430 DAOSDD010000000
VERSION     DAOSDD010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22050_length_1808_cov_5.005134"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PK733_20000"
     CDS             complement(<1..1156)
                     /locus_tag="PK733_20000"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ12855.1"
                     /translation="MSKLDELIAELCPDGVEYVRLAECCDIEDNKRKPVKSSLRIPGD
                     TPYYGANNIQDYVEGYTHYGQYVLIAEDGSASLENYSVQYVDGFFWANNHVHVIKGRK
                     DLDTKFLYYYLTSFNFIPFLTGGTRAKLNKGNMEEIPIPIPPLPVQQEIVRILDNFTE
                     LTAELTAELTAELTARRKQYEYYRDLLLTFGDDVPMMALGDICTVQSGGTPSKANHEY
                     WDNGTIKWLGSTVCQNQKTVDEVTNYISELGLKNSSSKLMKKETTLIALVGATIGKVA
                     FLPFEAAINQNIAGIYPIDTSRINPSYVFYACSMLYSDFIGLSQGKLAMANLSFVRGL
                     KIPVPPLKEQERIVAILDRFDALCNDLTSGIPAEIEARQKQYEYYRDKLLS"
     gene            complement(1153..>1808)
                     /locus_tag="PK733_20005"
     CDS             complement(1153..>1808)
                     /locus_tag="PK733_20005"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOJ12856.1"
                     /translation="SNPPYSIKWKGDNDPILINDPRFSPAGVLAPKSKADLAFIMHSL
                     SWLATNGTAAIVCFPGVMYRGGAEKKIRQYLIDNNYIDCIIQLPDNLFYGTSIATCIM
                     VLKKSKSENSTLFIDASKEFVKVTNNNKLTQENIETILNAFKERKDIEHFARLVPNSE
                     IAEQDYNLSVSTYVEQEDTREKIDIVALNAEIEKIVAREQVLREEIDKIIAEIEVGK"
BASE COUNT          515 a          377 c          326 g          590 t
ORIGIN      
        1 aagataacag tttatctctg taatattcat attgcttttg ccgtgcttcg atttcggcag
       61 gaatgccgct ggttaagtcg ttgcagagag cgtcaaagcg atctaggata gctacgatgc
      121 gttcttgttc tttgagagga ggaacaggga tttttagtcc tcttacgaaa gataaatttg
      181 ccattgcgag tttaccctga ctaagtccaa taaagtcaga atataacata ctacacgcat
      241 aaaatacata tgaaggattt attcttgaag tatcgatcgg gtatattcca gctatgttct
      301 gattgattgc agcttcaaac ggtaaaaaag ctactttgcc aatagttgct ccaactaaag
      361 caattaaagt agtttccttt ttcattagtt ttgatgaaga atttttcaaa cctagttcac
      421 taatatagtt tgttacctca tccacagtct tttgattttg gcacacagtc gagccaagcc
      481 attttattgt tccattgtcc caatactcat gatttgcctt tgatggagtg ccccctgact
      541 ggacagtgca aatatcgcca agagccatca ttggtacatc atcaccaaac gtcaatagca
      601 aatccctata atactcatac tgctttcttc ttgctgtaag ctctgctgta agctctgctg
      661 taagctctgc tgtaagctcg gtgaaattgt ccagaatacg gacaatttcc tgctgaacag
      721 gtagaggggg aatggggatt gggatttcct ccatattccc cttatttagt ttagctcttg
      781 ttcctccggt caagaagggg ataaaattaa aacttgttaa gtagtaatat aaaaacttgg
      841 tatctagatc cttacggcct tttataacat ggacatggtt gttagcccaa aagaaaccat
      901 ctacatattg tacagaataa ttttcaagac ttgcagagcc atcttctgca attagtacat
      961 attgtccata gtgcgtatat ccctcaacat aatcttggat attgttagca ccataatacg
     1021 gagtgtcacc tggtatgcgt aatgatgatt taacaggctt ccgtttattg tcctctatat
     1081 cacaacattc cgcaagtcga acatactcca ctccatctgg acaaagttca gcaatcaatt
     1141 catctaattt actcatttgc ccacctcaat ttctgcaata atcttatcta tttcttccct
     1201 taaaacctgc tctctcgcca caatcttttc tatctcagca ttgagggcaa caatatcaat
     1261 cttttctctg gtatcctctt gttccacata tgttgaaaca gacaggttat aatcttgttc
     1321 agctatttca ctgtttggca caagcctagc aaaatgctct atatcttttc tttccttaaa
     1381 tgcattaaga atggtctcaa tattttcctg tgttagcttg ttgttattcg taaccttaac
     1441 aaattccttt gatgcatcga taaataaggt gctattttcc gatttagatt tctttagaac
     1501 cataatgcag gtagcaatgc tggtaccata aaacaagtta tccggtaact gaatgataca
     1561 gtcgatatag ttattgtcaa ttaggtactg cctgattttc ttttcagctc caccacggta
     1621 cattacaccg gggaaacaaa cgatagccgc tgttccgttt gtcgcaagcc aagaaagact
     1681 atgcatgata aaagcaaggt ctgccttgga tttaggagcc aatactcccg ctggagaaaa
     1741 gcgggggtca tttatcaaaa taggatcatt gtcgcccttc catttaatgg agtaaggtgg
     1801 atttgata
//
LOCUS       DAOSDD010000431         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_23424_length_1724_cov_4.354703, whole genome shotgun sequence.
ACCESSION   DAOSDD010000431 DAOSDD010000000
VERSION     DAOSDD010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23424_length_1724_cov_4.354703"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PK733_20010"
     CDS             <1..971
                     /locus_tag="PK733_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ12857.1"
                     /translation="PTAVSFVLNNKKGVSEKTRKKVREVIEKTNFVPSLNSRRLFFKK
                     SYNISLVIQQSSSPFSDLFYYEITNGILEKSKEFGYNIVFTDISMKGNSMEIPNIIKY
                     KDTDGIIFYQDTERAILREVDKLNIPYVIVDAHSTSSDFTSINADSELLSYTATKFLI
                     ERGHVDIALISSSYIPEYYIQIFTGYKNALEEVRVSISPYWIQNNAFNELSAYNCMEN
                     ILKNALKPSAVFCAGDIFAIGAIRCAKDKGYRVPEDISFIGVDDIILSRYIEPALTTI
                     RIDKALMGRLAMDLLVKKINGESVESIILESDNIVVRNSVKNMDCS"
     gene            1502..>1724
                     /locus_tag="PK733_20015"
     CDS             1502..>1724
                     /locus_tag="PK733_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOJ12858.1"
                     /translation="MGYIKTVVPMKDYRLFMDMESGSNVIVDLSVKLNTMKYKDLTDE
                     QIFRSAKTDGDYVVWGDGRVKVTVKELMEV"
BASE COUNT          568 a          275 c          328 g          553 t
ORIGIN      
        1 cacctactgc tgtgtccttt gttctaaata ataaaaaagg tgtaagtgaa aaaacaagaa
       61 aaaaagtcag agaagtaatt gagaagacta attttgtacc cagtcttaat tcccgaaggc
      121 ttttttttaa gaaaagctat aatataagtc tggtgataca gcaatcttct tctccattct
      181 ctgatctttt ttattatgaa ataacaaatg gaattcttga aaaaagcaaa gagtttggtt
      241 ataatattgt ttttacagac atttccatga agggcaactc aatggagatt cccaatatta
      301 tcaaatataa agatactgat ggaattatat tctaccagga taccgaaaga gctatattaa
      361 gggaagtcga taaattaaat ataccttatg tgatcgtaga tgcacattca acaagcagcg
      421 actttacgtc aataaatgct gacagtgagc ttttgtcata taccgcaaca aaatttctta
      481 ttgaacgagg gcatgttgat atcgctctta ttagttccag ttatatccct gaatactaca
      541 ttcaaatatt taccggatat aaaaatgctc tggaggaagt tcgtgtctcc atatctcctt
      601 actggattca aaataatgca tttaatgaat tatctgccta taattgtatg gaaaatattt
      661 taaaaaatgc gttaaaacct tctgctgtct tttgtgccgg tgacattttt gctattggtg
      721 caattagatg tgcaaaggac aaaggttata gggttccgga agatatttct ttcatcgggg
      781 tagatgatat cattttatcc aggtatattg aaccggctct tacaactatt agaatcgata
      841 aggctctcat gggccgtctg gcaatggatt tattggtaaa aaagataaac ggtgaaagtg
      901 tagaaagcat catcctggaa tcggataata ttgttgtcag gaattcagta aagaatatgg
      961 attgtagtta aaatggggat ttatgccaat acatcaatta ctatacatta tcgttaaact
     1021 cttttatata agaacatatg cctattttca aagctaacgc ctccctttaa tgaatataat
     1081 actatcgaaa actataaatg ctttcgatat ctatgtttcg aggtttgcgc tttttctcat
     1141 acctccaaca tttcccatat atttacttta atcccgaact tttctgaaat atgtaatatt
     1201 ttattttaag aaatagcata aatacttgtt tttacatatt ttttatttgc ccttcatatt
     1261 acgtaaatac gtaacatatg agacaaataa taggttatct aaagtgcatt agggttgcat
     1321 attacgcaaa aatcgaaaag tttgggtcta aaagatcatc ctgaaagatc atccatttgt
     1381 acgaggcata ttccaaaatc ctgttttaca atgatttata gaatgggttt ttagtttagc
     1441 cgggatcgct gtgaagcaac cccggcaatt gtgattggct atggtgaaag gagatcacaa
     1501 tatggggtat atcaaaacgg ttgtgcctat gaaggattac cggctgttca tggacatgga
     1561 aagcggcagt aatgtgatcg tggacctctc ggttaagctt aacaccatga agtataagga
     1621 tctgactgac gagcagattt tccgcagtgc caaaacagac ggcgattacg tggtatgggg
     1681 agatggacgc gtcaaggtaa cggtcaagga actgatggaa gtag
//
LOCUS       DAOSDD010000432         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_25606_length_1604_cov_4.322789, whole genome shotgun sequence.
ACCESSION   DAOSDD010000432 DAOSDD010000000
VERSION     DAOSDD010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25606_length_1604_cov_4.322789"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PK733_20020"
     CDS             complement(<1..564)
                     /locus_tag="PK733_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12859.1"
                     /translation="MKKIKENLLTIGWSLRLALSINAKIFLLWSTLSILLAVLPAVAL
                     YFNRETVFVLSNFLLTRHGEFRDVLPSVVSLGVVMTAMGVSKRINGNFLYFIMYDSYY
                     FGLEEHMMDVVRKIEIKTLMDKKYRDDHWSAMGRCGALADFMSSGCLFLSKLAGAISL
                     LVVAAQTSWVIFAFSAASFNRCNAWRSF"
     gene            complement(919..>1604)
                     /gene="fbaA"
                     /locus_tag="PK733_20025"
     CDS             complement(919..>1604)
                     /gene="fbaA"
                     /locus_tag="PK733_20025"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ12860.1"
                     /translation="RSVSKSNLFQSLMFDGSSLPIDDNLQISKGLIETCRKNEIILEI
                     EIGVVGGEEDGFRTDGVASSRLYTTPEDMIKVYEVLEEVHSSKYMVAATFGNIHGVYK
                     PGSVKLKPEILKHGQDAIKKKYGKDAGLYLVFHGGSGSTREEVQETLEYGVVKMNIDT
                     DTQYAFTRPIADHMFKNYDGVLKIENEVGNKQLYDPRVYLKLAEKSMSKRIVEACEVL
                     RSEGKTLCI"
BASE COUNT          486 a          363 c          263 g          492 t
ORIGIN      
        1 aaaagatcgc caggcattac agcggttaaa agaagcagct gagaaagcaa aaataaccca
       61 tgatgtttgc gccgcaacga ccagcaacga aatagctcct gctaatttcg atagaaataa
      121 gcatccggag ctcatgaaat ctgcaagtgc tccacacctg cccatagcag accagtgatc
      181 atcacggtat tttttatcca tcagagtctt aatttcaatt ttacggacaa catccatcat
      241 gtgctcttca aggccaaagt agtaagagtc atacatgatg aagtagagaa aatttccatt
      301 aatcctctta gaaaccccca tggctgtcat aacaacacca agcgaaacaa ccgatggcaa
      361 aacatcacga aattcaccat gcctagtaag caagaaattg gaaagaacaa aaaccgtttc
      421 acggttaaaa tacaacgcta ccgcaggtag gacagcaaga aggatgctta atgtgctcca
      481 taataaaaat atttttgcat tgatgctcag ggcaagtctt aaagaccagc cgatcgttaa
      541 tagattctct tttatctttt tcaaaactga accccctcat caaacattga tttaattctc
      601 ttcaaaactt cagagcgccg tccccggctg acaggtataa gttttcctga agccaagata
      661 agatcattag attccaactt ttcaaagaat agcaggttga ctatgaatcc cgcatggcac
      721 cgaataaaat acggctcaaa caatgcctgt tcgtgatcct tcattcgttt ataactaaga
      781 atggagtctg tctccgtatg aatgcgggta ttgtgctcct cagtttcgat atattgaatg
      841 gatttagtgt atattttata gactccatta gcatttgttt cgatgaaaaa tcgattttgt
      901 ccctgcatat tataccaatc aaatacataa tgtttttcct tcagaacgta atacctcaca
      961 tgcttcaaca atacgtttgc tcatgctctt ttctgcaagt ttcaaatata ctctgggatc
     1021 atacaactgt ttattcccta cttcattttc tatttttagt acaccgtcat aattcttaaa
     1081 catatggtct gcaataggtc ttgtaaaggc atactgagta tcggtatcaa tattcatctt
     1141 gaccacacca tattcaagag tctcctgtac ttcttcccta gtcgaaccag agccgccatg
     1201 gaatacaagg tacagtcctg catctttgcc atatttcttt tttattgcat cctgcccgtg
     1261 tttcagaatt tcaggcttaa gctttacact tccgggtttg tatacaccat ggatatttcc
     1321 gaaagttgct gcaaccatat attttgaact gtgtacttct tcaagtactt catatacttt
     1381 tatcatatct tcaggtgttg tatatagtct agatgaagca acgccgtctg tccggaagcc
     1441 atcctcttcc cctcctacaa caccaatttc aatctcaaga atgatttcat tttttctgca
     1501 tgtttcaatt aggccttttg atatctgcaa attatcatca attggcaagc ttgatccgtc
     1561 aaacatgaga ctttgaaaaa ggttactctt gcttactgac ctcc
//
LOCUS       DAOSDD010000433         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1840
            NODE_26623_length_1556_cov_4.064624, whole genome shotgun sequence.
ACCESSION   DAOSDD010000433 DAOSDD010000000
VERSION     DAOSDD010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863369
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:11:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,006
            CDSs (total)                      :: 3,966
            Genes (coding)                    :: 3,900
            CDSs (with protein)               :: 3,900
            Genes (RNA)                       :: 40
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 48 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26623_length_1556_cov_4.064624"
                     /isolate="AMR_MDS_1840"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1219)
                     /locus_tag="PK733_20030"
     CDS             complement(194..1219)
                     /locus_tag="PK733_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ12861.1"
                     /translation="MKQNMKKWILNLSNSIDRYAMPLMTYPGLEPANKTIMDITNDGE
                     AQFECIQALAEIYPSIGTVTVMDLSVEAEAFGSPVTFSDDEVPTVLSRIIYDRESAEA
                     LKVPKVGEKRTLAYIMAAELASKKIKDRPVFGGEIGPFSLAGRLYGMMETMVALLLEP
                     ETVHIILEKATSFLIEFAEAYKKVGANGIIIAEPAAGLLSPGQCEEFSSQYVKKIVDT
                     VQDDYFMVILHNCGNTRELIPSMLSTGAMGFHFGNAVDLKEILPQIPWGKLVFGNIDP
                     AGVFKNGSREDVKNETWRLLENTAIYKNFIISSGCDIPPGTTTDNIEAFFETLDKFNL
                     TFVRVTG"
BASE COUNT          486 a          304 c          229 g          537 t
ORIGIN      
        1 gagacaaata atagattatc caaagtgtat taggattgca tattacgtaa aaatcgaaaa
       61 gtttgggttt aacttaacac aagcgcctta ctatgttctt gtgtttttat ttgccgcaaa
      121 acaacggatt cctcactatt caaaatgcac ccttgcactg ccgtaaatat tgcaactgga
      181 aatgattcgc tacttatccg gtaacacgca caaaagtcaa attgaactta tccaaggttt
      241 caaaaaatgc ttctatatta tcggtagttg tacctggagg tatatcacat cctgaagata
      301 ttataaagtt tttataaata gctgtattct ccagtagtct ccatgtctca tttttcacat
      361 cctccctgct gccgttttta aatactcctg cggggtcaat atttccaaat acaagctttc
      421 cccatggtat ttggggcaat atctccttta aatctactgc attaccaaag tgaaagccca
      481 tggcacctgt agatagcatg gaaggtatga gttccctggt atttccgcag ttatgcagta
      541 taaccatgaa ataatcgtcc tgtaccgtat ctacgatctt cttaacatat tgagatgaaa
      601 actcttcaca ttgaccaggc gaaagtaacc cggctgcagg ctctgcaatt attatcccat
      661 ttgctccgac cttcttataa gcttctgcaa attctataag aaagcttgta gccttttcca
      721 atattatatg cactgtttca ggttcaagta aaagagcaac catagtttcc atcatgccat
      781 acagccttcc agccagcgaa aatggtccta tctcaccacc aaatacaggt ctgtctttta
      841 tttttttaga tgccagttct gctgccatga tatatgctaa tgtacgtttc tcgcctacct
      901 tcggcacttt taatgcttcc gccgattcac ggtcatatat aatcctgctc aatactgtag
      961 gcacttcatc atctgaaaaa gttacaggac ttccaaaagc ttcagcttct acagacaagt
     1021 ccataactgt aactgtccca attgaagggt atatttctgc aagagcttga atgcactcaa
     1081 actgagcttc cccatcatta gttatgtcca ttattgtttt atttgccggt tccaaccctg
     1141 gatatgtcat taatggcatt gcatacctat cgatagagtt tgataaattc aatatccatt
     1201 ttttcatatt ttgtttcata taaatcacac ccttctgtct aatataacat ctgatataat
     1261 tataaattca tgttcatatt ttttctcata atattttaat attctttgta ttttttttaa
     1321 caatgtttag tgtaaatgaa tatagagtaa aaaagtacaa aatatataaa atattttcaa
     1381 taccttatgg taagatacat agtagtatat atacatacag taatatttaa gcaaattaga
     1441 ctttgtaata gctaatgatt tgtggtatat ttaaagtata tattacaatt atacataata
     1501 cataaattga tttaatgaaa gtgaggtaaa taaatatatc atttttttct aatgac
//
